LOCUS       DAGSHT010000001        76219 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGSHT010000001 DAGSHT010000000
VERSION     DAGSHT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 76219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.001"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..291)
                     /locus_tag="MJB72_000001"
     CDS             complement(<1..291)
                     /locus_tag="MJB72_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261184.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNG"
     gene            complement(288..515)
                     /locus_tag="MJB72_000002"
     CDS             complement(288..515)
                     /locus_tag="MJB72_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261185.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            complement(755..916)
                     /locus_tag="MJB72_000003"
     CDS             complement(755..916)
                     /locus_tag="MJB72_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261186.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            complement(1029..1517)
                     /locus_tag="MJB72_000004"
     CDS             complement(1029..1517)
                     /locus_tag="MJB72_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBY8261187.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            complement(1542..2356)
                     /locus_tag="MJB72_000005"
                     /pseudo
     CDS             complement(1542..2356)
                     /locus_tag="MJB72_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2480..4345)
                     /locus_tag="MJB72_000006"
     CDS             complement(2480..4345)
                     /locus_tag="MJB72_000006"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY8261188.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            complement(4342..6405)
                     /locus_tag="MJB72_000007"
     CDS             complement(4342..6405)
                     /locus_tag="MJB72_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8261189.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            complement(7002..7880)
                     /locus_tag="MJB72_000008"
     CDS             complement(7002..7880)
                     /locus_tag="MJB72_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8261190.1"
                     /translation="MDSLRSMNNALEYIEEHLTEEIDYIEVSKIAYCSEYHFKRMFSF
                     LAGISLSEYIRRRRLTLAALDLKDRDLRIIDVAVKYGYNSADSFSRAFHSLHGILPSE
                     ARSENTQLKAYPRMTFQLSIKGGCEMNYRIVEKGPFKLVGFKKRVPIIFEGVNPEIAK
                     MTELLTMDVINQLKAISNVEPTGIISASTNFSEGRMEEKGELDHYIGVATSSNETADF
                     DVLKIDASTWAVFESIGPFPETLQNVWGRIYSEWFPSSGYEAVEGPEILWNESPDTGN
                     PKYRSEIWIPVKKKDY"
     gene            8090..8230
                     /locus_tag="MJB72_000009"
     CDS             8090..8230
                     /locus_tag="MJB72_000009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261191.1"
                     /translation="MTLSSKSASSSLALLSSLIRSNSSLPNPERESLIERAVSFPPLN
                     SN"
     gene            complement(8246..8740)
                     /locus_tag="MJB72_000010"
     CDS             complement(8246..8740)
                     /locus_tag="MJB72_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_022071788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HBY8261192.1"
                     /translation="MVSKTDAIEIITYAEENDINIWIDGGWGVDALLEEETRVHNDID
                     LFVEESNGKKFIEILKEYGFAEVIEAYTTTSHTVYKDSKGRIIDLHIFKFNEQGYIVF
                     EGEAYPPEVFSGIGKIGDKTVRCIDAENQVLFHLGYEHDENDMHDVKLLCERFNIPVP
                     SEYK"
     gene            complement(8939..9577)
                     /locus_tag="MJB72_000011"
     CDS             complement(8939..9577)
                     /locus_tag="MJB72_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vat family streptogramin A O-acetyltransferase"
                     /protein_id="HBY8261193.1"
                     /translation="MIGPDKKKLYPNENIKTVCFISNLPKRPNVEIGEYTYYSDNKKT
                     PEKFYDNIEHHYEFLGDKLIIGKFCAIAEGVKFIMNGANHRMDGITTYPFNIFGHGWE
                     KVTPTIEQLPFKGDTVIGNDVWIGQNVTIMPGIKIGDGAIIAANSTVVKSVEPYTIYG
                     GNPAKFIKKRFSDEKIEFLLKLQWWNWSEEEIFNNLEMLTSKAGLEQFMKNS"
     gene            complement(9785..11356)
                     /locus_tag="MJB72_000012"
     CDS             complement(9785..11356)
                     /locus_tag="MJB72_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8261194.1"
                     /translation="MAVSRNVTVIPAIKRVGNNKNSESKSKIRVAAYCRVSTDSEEQA
                     SSYDIQIEHYTNYIKKNKEWELAGIFADDGITGTNTKKRDEFNRMIEECMAGNIDMII
                     TKSISRFARNTLDCLKYIRQLKDKNVAVFFEKENINTMDSKGEILLTIMASLAQQESQ
                     SLSQNVKLGIQYRYQQGEVQVNHKRFLGYTKDENKQLVIEPKGAEVVKRIYREYLEGA
                     SLLQIARGLETDGILTAAGKAKWRPETLKKILQNEKYIGDALLQKTYTVDFLSKKRVK
                     NNGIVPQYYVENSHEPIIPRELFMQVQEEMVRRANLRGGKGGKKRVYSSKYALSSIVY
                     CGHCGDIYRRVHWNNRGYKSIVWRCVSRLEEKGSECTAPTINEETLQTAVVRAINELL
                     ANKEPFLSTLQKNIDTIFNEESDNDTDELDSKLEELQIELLKQAKSKNDYDNVADEIY
                     RLREMKQNSLVENAEREGKRQRIAEMTAFLNEQSHELEEYDEQLVRRLIEKVTIRDDR
                     IEVEFKSGVKIEEMI"
     gene            complement(11360..11773)
                     /locus_tag="MJB72_000013"
     CDS             complement(11360..11773)
                     /locus_tag="MJB72_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8261195.1"
                     /translation="MGHTPYGYRIEDGKAVVDDIAAEKVRELFSGYLAGLSLKGAAKK
                     AGLDCYHATASKMLQNKHYLGDEFYPPIIDEETFEKARVEKRKRAEKLGRIWEPKDEP
                     ERDYPVKFKAKPLVQKYEDPYKQAEYAYSLIESEV"
     gene            complement(11774..13339)
                     /locus_tag="MJB72_000014"
     CDS             complement(11774..13339)
                     /locus_tag="MJB72_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8261196.1"
                     /translation="MKKITKIEGNTANSFIKPKTRVVAYCRVSTGSDEQLVSLQAQKA
                     HYESYIKANPEWEYAGLYYDEGISGTKKENRSDLLRMLSDCETGRIDLIITKSISRFA
                     RNTTDCLEMVRKLIDLGVHIYFEKENINTGSMESELMLSILSGLAESESISISENTKW
                     AIQRRFQNGTFIISYPPYGYQNIDGQMKVIPKQAEIVKYIFAEVLSGKGTQKVANNLN
                     QKGIPSKRGGRWTATTIRGILTNEKYTGDVILQKTYTDSHFNRHTNYGEKNMYLVENH
                     HEAIISYEDFEAVDAILNQRAKEKGIEKRNSKYLNRYAFSSKIICSECGSTFKRRIHS
                     SGPRKYIAWCCSKHISNITECSMQFIRDDDTKTAFVTMMNKLIFGQKFILRPLLNGLR
                     NQNNAASFRRIEELETKIENNMEQSQMLTDLMAKGYLEPALFNKEKNSLEAERERLLA
                     EKDQLTRSVNGNFAKVDEVDRLLKFATKSKMLTAYEDELFENYVEKIIVFSREEVGFE
                     LKCGITLKERLVN"
     gene            complement(13392..13622)
                     /locus_tag="MJB72_000015"
     CDS             complement(13392..13622)
                     /locus_tag="MJB72_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261197.1"
                     /translation="MQITKITDKQDQISISKKTLLSAEALQREFDYYRAEKLLSKMLE
                     KGLITEVEFNKIGALNRQTFSPFLKEIMPSNR"
     gene            complement(13754..14455)
                     /locus_tag="MJB72_000016"
     CDS             complement(13754..14455)
                     /locus_tag="MJB72_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8261198.1"
                     /translation="MNLRKLILTENACYKAGRKIIPKGIMVHSTGANNPYLRRYVGPD
                     DGLLGVNEYNNHWNQDKPGGRQVCVHAFIGKLKDGSIATYQTLPWNHRGWHAGGKAND
                     THIGFEICEDGLTDASYFNAVYKEAVELCVYLCKLYNLTEKDVIGHYEGYQKGIASNH
                     GDPKNWFPKHGKSMDIFRADVKKLLSEGEKPAEQEKKKYYRVQIGAYSVKANAEAQLA
                     KAKKAGFTDSFIKYD"
     gene            complement(14455..14862)
                     /locus_tag="MJB72_000017"
     CDS             complement(14455..14862)
                     /locus_tag="MJB72_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBY8261199.1"
                     /translation="MKEIWNGVQVALTALGGFLGWFLGGFDGFLYALVALTIADYITG
                     VMCAIVDKKLSSEIGFRGIFKKVLIFVLVGIGHMIDTNLIGEGSVLRTAIIFFYCSNE
                     GISMLENAGRLGLPIPEKLKDILVQLHNKGGNE"
     gene            complement(14941..17412)
                     /locus_tag="MJB72_000018"
     CDS             complement(14941..17412)
                     /locus_tag="MJB72_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBY8261200.1"
                     /translation="MIDYLKGKRCMVWSFMGNTRMYQALRDYGDRIDTVGIFTFEVDI
                     TGTITETGTSISSMLTYINRWPHIKWLLTIMNHGTASIFTALRNNTNGAKDKFLTEII
                     RIMNKYPWCAGVDIDLERGGGYENKDAANALFRDIYNSVKAYDSSKLVNICLPGMTGV
                     QGSVGGENWCVYEDLNAYCDTAAIMSYGMAWAGSAPGSVSPRDWLEGIYDYAVRVMTP
                     EKIFLGLPAYGWNWRIHDTPENLGITYRGISNTYYAAQLWMTGGYNFTDDGPPQPMIP
                     IIAYWDDYDKVPWALPHVYDYMEGWDAVSRTYPLLGETYNRRRYLTAYSKQQKTEFGT
                     IYVDRSGGTPDSYTGNVSVSSQMITLGEEGEAEYEFEISSAGIYDVAIRISFPFWDKN
                     SIHVSLDGTSKVFSENRLWWPYWRTTCWLSLVSGVFLSAGTHTVSVSTSVPGVQFYGF
                     RVCSSFSEEPSAGEAEFMLSPRKFKDVNGLMAEPDKGFKLTTEVLRRKPDSALIWYED
                     FRDDNPLPSSYWTALSGEWQVWQNQNDPASRPYSQLDGYGQLAWKYSGFSDVHLRARL
                     AFTPEGSGRAGIFCGNVFCCLNFNTQRIELYNGASLIGSYTTEIVKTASADLRSNPRM
                     YTIEMRIRGNSVRVYSGASNVLRFTATLSGFSGGYAGVRSDGRILCELLRLGDAWTYE
                     PYERFDVTFPDGTTTEYGRLARTGVTWDSEFQVFTVNNDVEEASTRNQDISMDYDFFH
                     SGLLPLVCGNDYSVKIVPKDINVWISRLFLGDADGFSILYYQDVDSLVYWANEAAYRW
                     KLRGIAIWSLGQEDMRLWEALPKQI"
     gene            complement(17409..17990)
                     /locus_tag="MJB72_000019"
     CDS             complement(17409..17990)
                     /locus_tag="MJB72_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261201.1"
                     /translation="MSVTFTRFAETIHCKEDKRVVSVTVKLLLGDCTGTVYFTDIQAQ
                     EGDRLTGYTINTETMLQKFREGGVIVPARFYNGVVRSGETVILFNLGSTSAGLDCHIY
                     PNQNMAAGSIQLSQGAGAHKVIFNEAVSPGDTFSLLASTRQCLKNGNPTDKEGFFQYT
                     ASGDSKHVIKLEDRKSARVLFEFQEMQEGSERL"
     gene            complement(18000..18194)
                     /locus_tag="MJB72_000020"
     CDS             complement(18000..18194)
                     /locus_tag="MJB72_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261202.1"
                     /translation="MAYFSQTAHAITPRGIGKIKSLTIRLFITDCSGEVFFTDMLLQG
                     GSVATGWIGHVSEIKWTLDG"
     gene            complement(18210..20732)
                     /locus_tag="MJB72_000021"
     CDS             complement(18210..20732)
                     /locus_tag="MJB72_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY8261203.1"
                     /translation="MAIKSILTSQTDFTGEFPVSEKTVALWRFNEAAPDSNNMLTDDS
                     GHNRNFFVSGWSGTSANLLSGRLGRYFRQNIINPTSEKTHLVATNDGSFFSDLGEKIV
                     VGGWINPTTYSVGQTYIPIFNTRQGPGQPIFYVSLFQGRLRLMLYNASGSLIYDQTET
                     PTITLKNNGWYFIASVIEVNNKRVQNLLCDRSDGAVWQSPVRTFTGDLNQSCVADIVM
                     GMHANTYYYAGGFDDWFYEKDSLLTMEDLISYFKSSILANGGDSAADVDALADPGSVM
                     LKATNGVYAQSGQLFTIAAACSLAGTGRVSVTSEYTAGITAISLVETSTSDDLSSWTE
                     WQAVGASGELQSPNREYIRYRITLSTQDTSRTPKLLEIQLHDIPKPPYERLGFARPVV
                     LDTNGAWEAVLENAFDIVVTSEVNGADILEFKLPFHDSKRETLDNEKQVQIVNDVYRI
                     RTITDEKSSDGRVVTQVYAEAAFYDLSFSAEKEPMEFVAETPEVPMRYALLGTGWSLG
                     NVTVSTKRTWQSTEKNALSILRAIQNIHGGDLIFDSANRLVHLLTFGGTDSGALFCYR
                     KNMKSIQRVVDTRSLITRLYAYGKDGITFASINGNKEYVEDYSYSSEVRVGTLDASSI
                     SNPYQLLEFANMRLAQYAKPRISYVLSAMDLSVLTGYEHEAWKLGDIVTVDDRDLKLS
                     VKTRVVRRQYNLQEPWKTVLELSTTLRELGDSSAGWDKAADILSSTDVLDRQELKDLV
                     PFNHLRNSRADDGLTYWLSSGFTVDPNNGVSGTASFKAEGVLGMTKSLSQTVYPASRK
                     SYTFSAQIASENLQKGPNGQVGIEVVIEYEDGSTETRFIDLF"
     gene            complement(20737..21510)
                     /locus_tag="MJB72_000022"
     CDS             complement(20737..21510)
                     /locus_tag="MJB72_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBY8261204.1"
                     /translation="MGFIYNGINSKNMKIKARLTSWQASPALRNAYEVVPGKAGVADF
                     GCDISERIITISCSIYPQKSFADLVSVLDDLAEWLNPMNGLKQLILEDIPNRFFFARL
                     TEQVECERLLRTAGAFELKFICPDPHAYALTDEQFTISSVGTHEIQRITGNTDSEPIY
                     QLKGSISGSTSTYISITTNGEELRVVGALTADEVLVIDSGLVTAKITDANGNTLRNGL
                     PVLDELNFPVLHKGENEITVSAVGATFSELKILAKSRWR"
     gene            complement(21524..24082)
                     /locus_tag="MJB72_000023"
     CDS             complement(21524..24082)
                     /locus_tag="MJB72_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBY8261205.1"
                     /translation="MADNFGLKIGVEGEKEFKNALRDINQAFKVLGSEMALVSSQFDK
                     NDKSIQALTARNEVLEKSIDAQKEKIETLRAALDNASTSFGENDRRTQNWQIQLNKAL
                     AELNGMERELENNHKALRDHADATDDSADNMEDAADAANELADNVDDVGDEMDDASKK
                     TSVFGDVLKANLLSEAIIGGVKALGSAIAGIGKAFIGAMKDGVEYNAQMENYTASFTT
                     MLGDEAKAQKLVNDLKKEAAATPFGMQDLAQSAQTLMSFGMSAEEAQKRMKQLGDISQ
                     GDAEKFKSLTLAFAQMSSTGKLTGQDLMQMINAGFNPLEEISRKTGKSIGELKDEMSK
                     GAISADMVAEAFASATSEGGRFYGSMEAQSKTFSGQMATLEDGVASLKGQLAEGLTTM
                     LSGTVLPMVNGWVDELSGAFEKDGVQGLIDAFGGILEEAVQFISEQLPIVVDIASQII
                     ISLVQGLTTALPQITEAAVMLLMTLVNGIIETLPALITAGIQMIGTIISGIAEALPQL
                     IPAAVSAVVQIVQGLLDNLPMVLEAALQLVLGLTQGILDALPVLIAALPAIITGIVNF
                     VIGAIPQIIEAGIQLLTSLIGALPEIITAIVEAIPQIVDGLITAILGSIPLLIDAGVQ
                     LLVALIQNLPLIITTVITAIPKIVSSLVNAIIGSIPQIIQAGIMLLVSLIKNLPAIIV
                     EVVKAVPQIIAALVKGFTGSIWQIAQVGTNLIKGLWQGISDAGAWLWDKISGFFGGVV
                     DKIKNFFGIRSPSTLFAGIGENMGEGIGVGFEKAMNSVSQDMQNAIPTDFDINPNLNM
                     NGAGISSGYGTFGGSLITIQQMIVRSEDDIRKISQELYNLMQTGSRAQGRFITA"
     gene            complement(24307..24681)
                     /locus_tag="MJB72_000024"
     CDS             complement(24307..24681)
                     /locus_tag="MJB72_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261206.1"
                     /translation="MDNERSAKINIGGQEYELILTTKATKEIAGRYGGLENLGEKLLK
                     SENFEMALDEIVWLITLMANQSLLIHNLRNPDNKKPLLTQEEVELLTSPLELATYKNA
                     LTEAMFKGTKRNVESEDDSKNV"
     gene            complement(24694..25296)
                     /locus_tag="MJB72_000025"
     CDS             complement(24694..25296)
                     /locus_tag="MJB72_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY8261207.1"
                     /translation="MATIGLDRLYYAKITEDINGDETYDTPMPLAKAISAELSVELAE
                     ATLYADDGAAEIVKEFKSGTLTLGIDDIGVAAAGDLTGATIDDNHVLISTSEDGGAPV
                     AIGFRAQKANGKYRYFWLYRVKFGIPATNLATKGDSITFSTPTIEGTVLRRNKLDGQG
                     KHPWKAEVNEGDEGVTSTVINSWFNEVYEPTFAASSGIGE"
     gene            complement(25300..25653)
                     /locus_tag="MJB72_000026"
     CDS             complement(25300..25653)
                     /locus_tag="MJB72_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261208.1"
                     /translation="MSILNELNLIADLCGIPVETGRFSGVPPDTYLVITPLIDLFEVH
                     ADNTPGYEVQEARLSLFVKGSYTAIKNAIVRTLLGADFTITDRRYIGHEDDTDYHHYA
                     IDVAKPYEFQLEKEE"
     gene            complement(25650..26081)
                     /locus_tag="MJB72_000027"
     CDS             complement(25650..26081)
                     /locus_tag="MJB72_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBY8261209.1"
                     /translation="MAKVQVKMPEDFLLKLSKLGDKTDEIIPKVLESGGEIVLEKVRS
                     NLQAIVGSGTKEKSRSTGELISSLGLSPAKVDRNGNFNVKIGFKEPRRSGESNAKIAN
                     IIEYGKSGQPPKPFLKPARRASRKACIDAMKKRFEQEVENL"
     gene            complement(26074..26409)
                     /locus_tag="MJB72_000028"
     CDS             complement(26074..26409)
                     /locus_tag="MJB72_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HBY8261210.1"
                     /translation="MSFGKMNTFIDLISVERTKDSEGFGKSKDTILASVRAYKEDRHG
                     NEKWANRATFSEATALFRFRKIPDVEVSTNMVIVCNDGRYEITNVEDVKGRGMYIEAM
                     AKKVVGSSG"
     gene            complement(26413..26721)
                     /locus_tag="MJB72_000029"
     CDS             complement(26413..26721)
                     /locus_tag="MJB72_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBY8261211.1"
                     /translation="MTLLEKVKANLILEHDRDDELLQMYITTAIAYAESYQHVPEGHY
                     NENTMPPTTEQAVIMLSSHFYESRDGSTGGFFADNVQAGQQVWNTVNLLLRLDRDWKV
                     "
     gene            complement(26739..26957)
                     /locus_tag="MJB72_000030"
     CDS             complement(26739..26957)
                     /locus_tag="MJB72_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Head fiber protein"
                     /protein_id="HBY8261212.1"
                     /translation="MSYSAKNYTEQGGEKTVIGGELVIEEGAKVTGLPVLENQPASTA
                     ETVEALVTDFNALLSKLKTAGIMNGDTP"
     gene            complement(26973..28172)
                     /locus_tag="MJB72_000031"
     CDS             complement(26973..28172)
                     /locus_tag="MJB72_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBY8261213.1"
                     /translation="MSKILELREKRAKAWEAAKAFLDSKRGSDGLVSAEDAATYDKME
                     ADIINLGKEIARLERQEALEAELNKPVNTPLTEKPAIPGMDTKTGRASDEYRKAFWNV
                     MRSKNPRHDALNALSVGTDSEGGYLVPDEFERTLVQTLEEENVFRKLAKIIQTSSGDR
                     KIPVVVTKGTAAWLDEGEEFDESDSVFGQTSIGAYKLGTMIKVSDELLNDSVFDLEKY
                     ISTEFARRIGAKEEEAFLVGDGDGKPTGIFNVTGGAQLGVSAGSATAITADEIIDLVY
                     SLKAPYRKNAVFLMNDATVKAIRKLKDGQGQYLWQPSLTAGTPDTLLNRPVYTSAYAP
                     IIEAGAKTIAFGDFGYYWIADRQGRSFKRLNELFATTGQVGFLASQRVDGKLILPEAI
                     KVLQQKA"
     gene            complement(28184..28864)
                     /locus_tag="MJB72_000032"
     CDS             complement(28184..28864)
                     /locus_tag="MJB72_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBY8261214.1"
                     /translation="MKKFWNWVRDADTQTRTLYLNGAIAEESWFEDDVTPAAFKEELM
                     SGEGDIVVWINSPGGDCIAASQIYNMLMDYKGNVTVKIDGIAASAASVIAMAGTEVLM
                     SPTSLMMIHNPFTIAIGDSEEMQKAIQMLDEVKESIINAYELKTGLSRTRLSHLMDAE
                     TWLNANKAVELGFADDIMFKPGESSLQDSFVFSRRAVTNSLMNKLQKPVVKQSVEPLY
                     ERLNLLKY"
     gene            complement(28861..30117)
                     /locus_tag="MJB72_000033"
     CDS             complement(28861..30117)
                     /locus_tag="MJB72_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY8261215.1"
                     /translation="MGLLSNIFKARDKPQNRTVGSNYSFFFGGTTSGKPVNEHTAMQM
                     TAVYSCVRILAEAVAGLPLHLYKYTESGGKEKAISHPLYFLLHDEPNPEMSSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVIALYPLMPNRMSVDRDSSGSLYYSYTRYSDEAPTM
                     NGMTVTLRPSDVLHIPGLGFDGLVGYSPIAMAKNAIGMAIACEEYGAKFFANGAAPGG
                     VLEHPGTIKDPQKVRDSWNAAYQGSSNSHRVAVLEEGMKYQSIGISPEQAQFLETRKF
                     QINEIARIFRVPPHMVGDLEKSSFSNIEQQSLEFVKYTLDPWVIRWEQAISRSLLRPD
                     EKKLYFAKFNVDGLLRGDYVSRMNGYATARQNGWMSANDIRELENLDRIPPELGGDLY
                     LINGNMTKLADAGIFANKEGMEGKPE"
     gene            complement(30136..31734)
                     /locus_tag="MJB72_000034"
     CDS             complement(30136..31734)
                     /locus_tag="MJB72_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBY8261216.1"
                     /translation="MRKLKKYKPTIFKADGSVYDKDAADIAVSFINCLKHTKGEWYGQ
                     PFELIDWQEQIIRDVFGIIKPNGYRQFNTAYIEIAKKQGKSELAAAVALLLTCGDFEH
                     GGEVYGCASDRQQASIVFDVAVDMVEQCPALKARIKPVLSQKRLVYKPLGSFYQVLSA
                     EAYTKHGLNVHGVVFDELHAQPNRQLFDVMTHGSGDARKQPLYFLITTAGNDTHSICY
                     EVHQKAKDILEGRKVDPTFYPVVYGADEDDDWTDPKVWAKANPSMGITVDIEKIQIAC
                     ESAKQNPAEENLFRQLRLNQWVKQSIRWMPMEKWDKCAFAINKESLEGRVCYGGLDLS
                     STTDITAFVLVFPPEYDDDKYIILPYFWIPEDNIEQRVRRDHVPYDIWEQQGFLYTTE
                     GNVVHYGYIENFIEELGLKYNIREIAFDRWGAVQMTQNLENLGFTVVPFGQGFKDMSP
                     PTKELMKLTLEEKLAHGGHPVLRWMMDNIFIRTDPAGNIKPDKEKSTERIDGAVATIM
                     ALDRAIRKGGSGNSVYDGRGLLIL"
     gene            complement(31805..31990)
                     /locus_tag="MJB72_000035"
     CDS             complement(31805..31990)
                     /locus_tag="MJB72_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HBY8261217.1"
                     /translation="MNDIIKSQIFAIRDTGETNMFDLPLLTSIALREGYIELIDYLEK
                     NKEAYVHFILTGKVETE"
     gene            complement(31983..32222)
                     /locus_tag="MJB72_000036"
     CDS             complement(31983..32222)
                     /locus_tag="MJB72_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HBY8261218.1"
                     /translation="MKTVHPKIIKQLRSVYTPGTRVVLVKMNDPYTKLEPGARGTVIG
                     VDDIGTIHVNWDSGSSLGVAYGEDSCRKIEEEKNE"
     gene            complement(32219..32911)
                     /locus_tag="MJB72_000037"
     CDS             complement(32219..32911)
                     /locus_tag="MJB72_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HBY8261219.1"
                     /translation="MKIEFNRTGSERKALVTAIGEILGEKPKYKGAPTFVYQIGRFEV
                     NKEGALIFDEGVVGEKATILLDELERQGFSYVKQDCLLAEHLEDKDLLVIEIPKRDFN
                     DKAYSNLEKILESKGALIKKALGVEELPVEQTEESLRFPWFYLDGDAEKVKAYTHFIT
                     ALCDMAKKQKRITATAKEADNEKYAFRCFLLRLGFIGEEYKTSRRILLSKLSGSSAFK
                     SGVPKREEVNVN"
     gene            complement(33008..34252)
                     /locus_tag="MJB72_000038"
     CDS             complement(33008..34252)
                     /locus_tag="MJB72_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HBY8261220.1"
                     /translation="MNIDKISIKKLIPADYNPRKDLRPGDPEYEKLKRSFEEFGYVEP
                     IIWNKTTGRVVGGHQRLKVLLSMGMDVVDCVVVEMDEEKEKALNIALNKISGEWDKEK
                     LALLITDLNAADFDVSLTGFDPGELDDLFKDYLKDKIKEDDFDIDSELNKPAISRLGD
                     VWILGRHRMVCGDSTNKETFNTLMDGKVANLVVTDPPYNVNYEGTAGKIKNDNMANDA
                     FYKFLLDAFINIETALATDGSIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLVL
                     GRSPYQWQHEPVLFGWKKKGKHLWYSDRKQSTIWEFDKPKKNADHPTMKPVSLIAYPI
                     MNSSLTNSIVLDPFGGSGSTLIACEQTDRICYTIELDEKYCDVIVKRYIEQVGTDKDV
                     YVIRQKEKIPFSGVATSSDEIC"
     gene            complement(34332..34925)
                     /locus_tag="MJB72_000039"
     CDS             complement(34332..34925)
                     /locus_tag="MJB72_000039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261221.1"
                     /translation="MNYEKVILEMLTRIKDLEEKVDMLQEYQQELQNKDEGEDDITSG
                     EEKKESGRKRTRREIMTILEEKYGYSVRKGNRSEGSGIVASKNGKSYNIKVSYSRSYT
                     DSDEVICSGWHTVFDKEIDNPDFPYFIFVVEGKESEFHYFIFKREDLINEFDDKAYDA
                     NKKLHFYFRVTKDGKPLEVRETEKDMTAYYNNWDIFK"
     gene            complement(35091..36269)
                     /locus_tag="MJB72_000040"
     CDS             complement(35091..36269)
                     /locus_tag="MJB72_000040"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBY8261222.1"
                     /translation="MSKRYLTAESVCAGHPDKLCDIIADNILDECLRRDKASRVACEV
                     MATKGKIIVAGEISCSEKIDIRSIVKNVLKELGYNPLKFLIYVYVHNQSSDIAAGVNT
                     ALEARNGINEQYGSIGAGDQGTMYGYATKETREMLPLPLVLSHRIVKRLDEARKGKII
                     KGILPDGKAQVTVEYHDDVPVRVKTIVVSVQHEKNKTQEELKSDILNNVLWQCFEDFP
                     FDDETEILINPSGQFVLGGPAADTGLTGRKIMVDTYGGLASHGGGALCGKDPTKVDRS
                     GAYMARYIAKHIVWCDLAEKCEVAISYAIGKANPVAFSINTFGTGTVSDEVLTLAAQE
                     VFNLRPAAIIEKLRLRNIHYSDTAVYGHFNSCLFPWEDVDRYNELKAAVEKYVDREYV
                     "
     gene            complement(36284..36835)
                     /locus_tag="MJB72_000041"
     CDS             complement(36284..36835)
                     /locus_tag="MJB72_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBY8261223.1"
                     /translation="MAKDGTNRGGARIGSGQKKKALIDKIAEGNPGKRKLEVIEFKNT
                     AELQGQEMPQPRAMLSAVQKDGKTLVASEIYELTWKWLEERGCAHLVLPQLLERYAMS
                     AARWIQCEEAISEFGFLAKHPTTGNAIQSPYVSMSHNFMSQTNRLWMEIYQIVRENCA
                     TEYSGTNPQDDVMERLLTARRGK"
     gene            complement(36947..37306)
                     /locus_tag="MJB72_000042"
     CDS             complement(36947..37306)
                     /locus_tag="MJB72_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBY8261224.1"
                     /translation="MPKKPKRPCSYPGCPELTDGRFCPEHTKKEASRYEKYQRDPETR
                     KRYGRAWKRIRDRYITAHPLCEECKRQGKLTPATEVHHILPLARGGTHDESNLMALCT
                     PCHSAITARDGDRWPSR"
     gene            complement(37426..37884)
                     /locus_tag="MJB72_000043"
     CDS             complement(37426..37884)
                     /locus_tag="MJB72_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261225.1"
                     /translation="MNIVWQYLDKRAAAINALKDYSSMKYIIEHTDEDIATLNEEMSS
                     PASPVINGMPSTHDPKAGEKRLIACINEIDVLKERYRQALEYMDWFQPAWDALTEDEQ
                     YVLKEFYLDDEQKQIDAVYNICDHFNIERSSAYNKKNRALQHLALLLYGK"
     gene            complement(37889..39238)
                     /locus_tag="MJB72_000044"
     CDS             complement(37889..39238)
                     /locus_tag="MJB72_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8261226.1"
                     /translation="MKYKPHEYQVYATEYILTHPIAAVLLDMGLGKSVITLTAIFDLT
                     LDSFLVRKVLVIAPLRVARDTWPAELEKWDHLKGLKYTVSVGSEVQRKTALMKRAQVY
                     IINRENVEWLISRSAIPFDFDMVVIDELSSFKSHQAKRFKSLMKVRPKIKRIVGLTGT
                     PSSNGLMDLWAEYRLLDMGQRLGRFIGRYREDFFVPDKRNQQVIFSYKPKPGAEEAIY
                     RLISDITISMKGTDYLKLPELVINEVDVKLSEKEMEILDIMKRDLITTVKGEEITAAN
                     AAALSGKLLQMANGAVYDDQGVVIHIHDRKLDALEDLIEAANGKPVLIAYWFKHDLSR
                     IQKRFEVEVLSTSDSIKRWNDGEIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQNETVVIHHLIAKGTIDERVMKALNDKNNTQSALIDAVKATLKEV"
     gene            complement(39219..39500)
                     /locus_tag="MJB72_000045"
     CDS             complement(39219..39500)
                     /locus_tag="MJB72_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBY8261227.1"
                     /translation="MREKWIEQQLVKAVKDIGGIALKIASPGFDGMPDRLILLPDKKI
                     AFVEVKAPGKTLRPLQEKRKRQLESLGFLVFCLDHIEQIGGILHEIQAS"
     gene            complement(39758..42103)
                     /locus_tag="MJB72_000046"
     CDS             complement(39758..42103)
                     /locus_tag="MJB72_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261228.1"
                     /translation="MKISVGNSRMDKKWKNKDITWEDFISRVKSTIRTTETVSEFRKM
                     SRAQQDSIKDVGGFVGGALREGKRRNGYVLSRSLLTLDMDYGKPGIWDQIDALHDFKC
                     CIYSTHKHTPDAPRLRLIIPLKREVTEDEYPALGRMVAKEIGIDLFDDTTYEPSRLMY
                     WPSTPSDGEFVFKEKDGELLDPDGYLSKYEDWRDTSMWPVSSRQSEVVQRKITKQADP
                     LSKEGVIGAFCRAYNIEEAIEEFLPDVYEPSTMNGRFDYIPADSSAGLVIYDGKFAYS
                     HHATDPACGMLLNAFDLVRVHKFRELDEKVTENTPPSKLPSFRAMTDLALEDERVKEQ
                     FAEERKAQAEKEFIDEDWEKQLEIDKTGTVKNTLRNLILILENDPNLKSIVFNQLSDS
                     LEIKGDVPWPHPSKFWRDADDAQLISYIDTHYGTFSARNYDVAVAKVADDRSYHPIRE
                     FIEALPEWDKVPRVDTLLIDYLGASDNPYVRAVTRKTLCAAISRVLTPGIKFDSMLVL
                     NGPQGVGKSTLIAKLGGDWFSDSLNLSDTKDKTAAEKLQGYWILEIGELAGLKKAEVE
                     TLRSFLSRQNDIYRASFGRRATPHLRQCIFFGTTNAEKGYLRDTTGNRRFWPVKTPGN
                     GTKKSWQLKQDEILQIWAEALTYVKAGEKLYLDASLEKLAKEEQREAMESDEREGLVR
                     EYLDMLLPEDWDNMDLYERRAYINGTEFGESQRVGVWKRKSVSNMEIWCECFGKDRAN
                     LRRVDGNEISAIMASIGGWTGLVKKERIQLYGPQWVYVPKE"
     gene            complement(42100..42519)
                     /locus_tag="MJB72_000047"
     CDS             complement(42100..42519)
                     /locus_tag="MJB72_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HBY8261229.1"
                     /translation="MDRYNAEGYPDPTAAEALENIMREEKAKNYKPCVFICSPFAGDI
                     EENLKKARGYLKFAVEQGTIPFAPHLLYPQVLDDSDPEQRKLGLFFGMVWLRKCEELW
                     VFGPYISKGMQAEIDKASKHRMAIRYFTENCEEVQKL"
     gene            complement(42523..43314)
                     /locus_tag="MJB72_000048"
     CDS             complement(42523..43314)
                     /locus_tag="MJB72_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBY8261230.1"
                     /translation="MNKLTIFNYEGNTVRTVMKDGSPWWVLRDVCSVLEIGNSRDVMA
                     RLDSDEKGVDIIDTPGGKQEVSIINESGLYSVILVSRKPEAKKFKRWVTHEVLPSIRR
                     HGLYATDELLANPDFLIQALQELKAERAKNAELTTTISIQEQQIAEMKPKASYYDVVL
                     NCKDAVSITTIAKDYGKSGRWFNEYLHNLGVQFRQGKIWLLYQKYAQHGYTTTKTHTY
                     LGNDGTMHSKVHTYWTQKGRLFIYELLKDHGILPLIEQESEFEEM"
     gene            complement(43403..45337)
                     /locus_tag="MJB72_000049"
     CDS             complement(43403..45337)
                     /locus_tag="MJB72_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBY8261231.1"
                     /translation="MRTLSIDIETYSSVDLAKSGVYRYAEAPDFEIILFGYSVDAGPV
                     QVVDLASGEAIPQEIQSAILDSKVIKWAFNAQFERVCLSKYFGVWLEPDSWRCTMVWS
                     AYLGLPLSLEGAAIITGVDKKKLTEGKELIRYFSVPCKPTRSNDGRTRNLPEHAPDKW
                     DRFKAYNLRDVEVELSIQEKLQKFPMPEEEWNNYILDQQINDRGIQLDLELVKKAIQC
                     DEKVREELTSRLKDLTDLENPNSVAQMKKWLSENGLETDSLDKASVKELLKDAPDHLS
                     EVLELRQLLAKSSVKKYSAMENAVCADGRARGLLQFYGANRTGRFAGRLIQVQNLPQN
                     HLLDLKQARCLVRGGHYEALELLYDSIPGVLSELIRTAFVPKKGYKFIVADFSAIEAR
                     VIAWIAGETWRNEVFATHGKIYEASASQMFKVPLEEVTKGSPLRQKGKIAELALGYGG
                     SVGALKAMGALDMGLTEEELKPLVYAWRNANPNIVRLWWDVDRAVKEAVTERCRTETH
                     RTRFEYRSGMLLIWLPSGRQLTYVKPRIGINSFGSESVTYEGIGGTKKWERIESYGPK
                     FVENIVQALSRDILCYSMRKLDEKDFDIVMHVHDEVVLEVPIEISVQDICALMGHTPP
                     WAQGLLLRADGFDCNFYKKD"
     gene            complement(45334..45894)
                     /locus_tag="MJB72_000050"
     CDS             complement(45334..45894)
                     /locus_tag="MJB72_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBY8261232.1"
                     /translation="MSTTAKRTNPTKVVTGVVRLSYANVWEPKSINGGAEKYSVSLII
                     PKSDTKTLSAINEAVNAAIEEGRGKFGGKIPNKAALKLPLRDGDIDRPDDEAYANSYF
                     INANSITAPQIVDRNVNPILERSEVYSGVYARVSINFYAFNSNGNKGIACGLGNIQKI
                     RDGEPLGGRTNAADDFATDVDDDFLS"
     gene            complement(45900..47027)
                     /locus_tag="MJB72_000051"
     CDS             complement(45900..47027)
                     /locus_tag="MJB72_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBY8261233.1"
                     /translation="MSKHAILSASGAHRWLNCTPSARLELEFDDNSGEAAAEGTAAHA
                     LSEHKLRKALKMRSKKPVSPYDSDEMDNYTDGYVEFVLEVIEQAKQACSDPLILIEQR
                     LDFSKYVPDGFGTGDCIIIADKVLNIIDFKYGQGVLVSAEDNPQMKLYALGALDLFDG
                     IYDIEMVSMTIYQPRRENVSTSTVSKESLYQWAEEVLKPKAELAFAGDGIYCPGEWCQ
                     FCRAAVKCRARAEAKMKLARFEFALPPLLTDEEITDILSSISDLTSWANEIIAYATDA
                     AVNHGKKWPGFKVVEGRSNRKYKDEEAVAQAAKNAGYQDIYRQSLITITEMEKLMGKS
                     KFNEVLGGLVMKPPGKPTLVPISDKRPEMNTSSAKNDFMEV"
     gene            complement(47024..47341)
                     /locus_tag="MJB72_000052"
     CDS             complement(47024..47341)
                     /locus_tag="MJB72_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HBY8261234.1"
                     /translation="MSKIKLLLDVVSDLRALADSVQAVADAIASKGPHEITKPEQPKP
                     EKQSEEKQLTLEEVRAVLAEKSHDGFTAEVRALLEKYGASKLSQIDPSKYAALLADAE
                     GLK"
     gene            complement(47334..47543)
                     /locus_tag="MJB72_000053"
     CDS             complement(47334..47543)
                     /locus_tag="MJB72_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261235.1"
                     /translation="MNLKHKVTINVARPGGERSSVIQSSRKTIRARMLDFLFGKKVSL
                     LVITPGDSVETVEIKEIKEGGVGHE"
     gene            complement(47661..48155)
                     /locus_tag="MJB72_000054"
     CDS             complement(47661..48155)
                     /locus_tag="MJB72_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8261236.1"
                     /translation="MSRAYKTSQNKRTNYIYYTAEGAKIVITPGEDGVTEAYIQLLHE
                     MDDVEVDEQRRYNYRITAHLDAYHDGEEEAAHDRNKYLADDTANPEQLIIQAEDEAEH
                     QDMLDKLTKAMECLLPQQKELFKKVYLEKRSNTDIATEEGVSEAAIRGRLKKLQERLR
                     KILS"
     gene            48640..48858
                     /locus_tag="MJB72_000055"
     CDS             48640..48858
                     /locus_tag="MJB72_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261237.1"
                     /translation="MAFSYNKLWKLLIDKKMLKKDLMEKTNLTSTTLAKMGKDLPVSM
                     DVLGRICKALDVNIGDIVDYVDDDTPKK"
     gene            48969..50120
                     /locus_tag="MJB72_000056"
     CDS             48969..50120
                     /locus_tag="MJB72_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261238.1"
                     /translation="MLLSVAPTYDISGEDGTVSDLILGKKNLSPIVTDTAPGADPHDI
                     SDYFKQSILQMLDSNKRSLIVLALKDIITSDNSIEPDTIVEIVNGMTKQDIMNRNAFV
                     LEEFLAGVFLYTVLYVENRNCESSVSEITDEYIQAFEDQKLDISFIKTYSNLSQDTAY
                     EIAIDAHSLVLLAETGGKCQKCGRVLGIKKEGNDINYAKVVRLSETDEVVLCVECERE
                     IQSASEEEKLALLSDKRDLENLIIARDATSRYTIERQIEQVLREVNLMDVTDDTQLKI
                     EPVKVENKITEKRLKDRVLFDVRQLYEGVNDSLYRLAGENRLNVDKFAKNVRRMYEDA
                     SESHISQSEIYYLLVETLFEKTGRKYRGACEIIISYFVQRCEVFDEITK"
     gene            50104..50343
                     /locus_tag="MJB72_000057"
     CDS             50104..50343
                     /locus_tag="MJB72_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261239.1"
                     /translation="MRLPNKVIPYTNSVIALFPDILEALTQQDMSPKELFEITAFRKK
                     DIADFLSALNCLFALGKIELIEEGRVLRYVKGNSM"
     gene            50321..51961
                     /locus_tag="MJB72_000058"
     CDS             50321..51961
                     /locus_tag="MJB72_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HBY8261240.1"
                     /translation="MLKEIQCDKFAPDHRVIRFNPGLNTVLGSAGGSNAIGKSTFLWI
                     IDYVFGGESYYSLTDDIKKEIGPHIIYFTFEFDEQPYYFYRSTDDPKNVYRSDEERRF
                     ITKLSLEDYRKFLFQEYNIGLPALTFSEITERFFRIYGRENTLEKYPLLVKPREQDEK
                     AVDFLLKLFGHYKILASIKSMEEELGIKSSQLKSRHRQKVDIEKIESNQKTIESLRKR
                     LQKLMKNSEEAQLALFGFDTQTFERVTAVQKELNSFVRKRNRLQSQLNAIKSNIADSK
                     ADTASEFESLIRFFPNANIKAFEEIEHFHRKIREILGEEMNQEIERLQPLINQYDKEI
                     MRLNRKIEETGIAKEMSERVLSQCVNVSKTIDKLEEETAELIHQKELQEARAEAEQTL
                     EKLFQQQNEKLDEIQDDINLRMDIINGVVTDQQETAPLLYISPHKEIFFETPGNTSEG
                     TAFKSLIVYDLSILELRPVPALIHDSNILKRIEDIHLEHILERYQSSGHQVFIAFDKA
                     DSTTEKAHKILEETAVLRLSDGNELFGRSWSKYESNDY"
     gene            51981..54290
                     /locus_tag="MJB72_000059"
     CDS             51981..54290
                     /locus_tag="MJB72_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBY8261241.1"
                     /translation="MAAINDLLRQIPDTSLRSRLEQEFARISKNKKFGLVFEEHIPEC
                     TPLYNVPIKRGSTVALKTGHINDVYTVLNLDGDNALCYNKSTGDTTNIPLADLVSVAQ
                     FGEPIFPTLQPIASVENAPDSSLWHTLIEADNYHALQLLEYLYPKKVDCIYIDPPYNT
                     GARDWKYNNDYVDSSDNWRHSKWLSMMQKRLKIAKRILADDGVLITTIDDNEYAHLWV
                     LLHELFPNLTHTCITIQHNPGGTQGKKFSVTHEYAIFSYSADSTIYRKQHTGGDVYNL
                     RRWGSTSGRYEGATCFYPVILDSNYNIIGFGDLLDKDLHPTSQVEHNEDGTIYVWPID
                     KNGIEKKWRYGRDTVESVRDRMYIERRGDRIEVILRRESEPPKTVWTDPLCNAEAHGT
                     DMIKAILGGGFSYPKSLYAVREALTFAVSGKKDALIVDFFAGSGTTLHAVNLLNAEDN
                     GNRRCILVTNNEVSDDESKTLKKNGFKPGDIEWEKHGICRAITWPRTKYSILGRRDDG
                     SILAGEYFTTQTVSDEVERSFYQLSFVDNPTELTVTAKKQVVSLLRNKEGKSQLPQSL
                     VTKDCKFIVSNKHTASILFDVSAVDEWLSAIEEQDQITDFYIVSKSTATFKSIKTRVS
                     ELLGPIIVTSQVKRPMSDGFPANAEYFKLEFLDKNLVSLGQQFHEILPLLWLKSGAIG
                     KRPEVNYNEEPEMLILPQNSFAILVDETRFSEFVKKLSEESGIETVYFVTNSEEAFRE
                     MTAGVKVNNTYQLYRDYIDNFVLGSRRDS"
     gene            54291..56798
                     /locus_tag="MJB72_000060"
     CDS             54291..56798
                     /locus_tag="MJB72_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBY8261242.1"
                     /translation="MRDILFPFQETALSELHDKINKAHLMWSERDPQIISFSAPTGSG
                     KTIIMTTLFEDILYGNADNIGEPDSVFIWLSDSPELNEQTRLKIESKSDKIRVRDLVT
                     IDSTFNAEYFEGGCIYFLNTQKLGSDKLLTGTSDNRQYSIWETLTNTAKRIPKKFYVV
                     IDEAHRGTYTSMQAENKAQSIMQKFIKGSEDDGLCVMPLVIGVTATPQRFDSLIAGTT
                     STVQKVIVPPEHVRESGLLKDRIIIHYPDIQLNADMTMFKGAVDNWRKKCIHWKTYCE
                     REDEKMVNPILVIQVEDGNDRIATQTDLGACIDLLEETLGRKLQPGEAVHTFNDRGTL
                     KVRDVEIQQIEASRIQDEENVLVVFFKMNLSTGWDCPRAETMMSFRSAQDYTYIAQLL
                     GRMIRTPLARRISSDAELNSVSLFLPYFDEETVKNVVNALRDSEAAMPTEAGTSKELV
                     TLGRNLAYSDVFEAMDNLITYRIDSSRKQAPLKLLIQLSRALTMDGIDLGAQKAVKNA
                     VLSKMDDEILRIKESGDYDTRGASITGFALGTLIFDYGDNAYSFDEETQTMTMSEFDI
                     SRHFEQAGKLLGEGLHKEYWIRHSTRDHIDVKKEIIILTNDTDAMERINDYAEKEFIS
                     LYENNKRSIARLSEARKNVYERLTNASVQPISVPWILPNSIDFSVPENSMKFEQHLYC
                     SEDGTFETSLNTWEIGVVTEELKNGAVCWLRNLDRKKWSLEIPYEVGGVTTSMFPDLV
                     IVRSDAQGYVFDILEPHDPSRKDNYPKAVGLAKFAEKHWDKFGRIQLIRLKKGVDGRE
                     HFYRLDMGKTTIRNKVRGITSNEELDRIFDTDAVRED"
     gene            complement(57020..57847)
                     /locus_tag="MJB72_000061"
     CDS             complement(57020..57847)
                     /locus_tag="MJB72_000061"
                     /inference="COORDINATES: protein motif:HMM:NF012797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261243.1"
                     /translation="MIKNRVVKISKEDNDFQHFEVIKRNRNKRHKHKEFFVEGVRNIN
                     EAIRNKWEISSFIYSGENELSGWAKSILSNVKVSFQYELPNHLMDKLSRKMDTSELLA
                     IVKMKTNDLSRIKLTKKNPLIVVMDRPSNKGNLGTLIRSCDALGANGLIITGHAVDLY
                     DPETIASSVGTFFSFPVLNLPSHKELYEWIQSLKSQHPNFQIIGTSAHGNKIINQCDF
                     LKPTVLLLGNETLGLSRNYKEISDLLVEIPMDGVASSMNVSCAASIVLYEIKRQRSL"
     gene            complement(57972..58700)
                     /locus_tag="MJB72_000062"
     CDS             complement(57972..58700)
                     /locus_tag="MJB72_000062"
                     /inference="COORDINATES: protein motif:HMM:NF023031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261244.1"
                     /translation="MKYKFETIKKDYSDFSSGRVLYNAANTTAFPVRLASEVIQRAFH
                     ILDKDGASGPYKIYDPCCGGGFLLTTIGFLYHNLISELIATDYDVEVLEIAKKNLSLL
                     SEEGLDRRMKEIKGYIEVFRKDSHIQALKSVNQLKTLIGEANINIHCAQRDITDMSAF
                     PIKEVNIIITDIPYGINVEWNSGSNNPIQNLFENSYKALDKNHSLMVVIADKKSKLEH
                     KLFKRVEYFKLGKRQVAFFKPNRC"
     gene            complement(58779..59657)
                     /locus_tag="MJB72_000063"
     CDS             complement(58779..59657)
                     /locus_tag="MJB72_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8261245.1"
                     /translation="MKPLKQLNLAMTYIEDNLSKEIDFQMVAQLACCSEYHFRRLFSF
                     LSGMTLSEYIRQRRLSQAALELCNADVKIIDVAMKYGYSSPDAFTRAFQGFHGVTPRE
                     AKTIGTALKAIPPMTFQLTIKGGSKMDYRIVEKEAFYIVGIKRRITLIYRGVNPDVKA
                     MYLSLSKEDFRKLAQLSNMELEGIVNASVNFEVDRFEGSSLDHFIGVATTIKPEGKWD
                     VLSVPASTWAVFTVEGTFPEALQTTWAKIASEWLVPQVSGYELNEGPELLCYNKDEYL
                     DSSNFRCEIWIPVVKK"
     gene            complement(59918..60826)
                     /locus_tag="MJB72_000064"
     CDS             complement(59918..60826)
                     /locus_tag="MJB72_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBY8261246.1"
                     /translation="MMFIATKTDDGEIKGKISFYCRVLHVSRQAFNKYLKAKDAPWKY
                     QELADAMLDICSEDECNDTYGRIRMYQALQLKQPEGIHIPGERTVYRVMDEIGLNHKP
                     KRKPNGITKADKEARKSDDLIKRDFTAEKPLKKCITDMTEIKASDGKLYVSAIFDCYD
                     LAVLGLAMDTNMKAPLCEQTLDNAYRAYPMLRGAILHSDRGTQYTSGLYRKAINKYGI
                     LQSMNSAGGRCHDNARCESMWARFKEELLYGRYDTSSMTVEQLKTLIWRYFIGYWNNR
                     RICSANGGLPPMVKRQQYYDSLQEAA"
     gene            complement(60823..61231)
                     /locus_tag="MJB72_000065"
                     /pseudo
     CDS             complement(60823..61231)
                     /locus_tag="MJB72_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215340.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            61623..62102
                     /locus_tag="MJB72_000066"
     CDS             61623..62102
                     /locus_tag="MJB72_000066"
                     /inference="COORDINATES: protein motif:HMM:NF018184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY8261247.1"
                     /translation="MKYKLENKGTFKVVSIRETITPNEKGIFVPNINQTTRENFCQYF
                     KQLSERRVGEILYIAVKQWEELNDYYVGVEISEKFSNPLPESLVELEIPMQTWAVFEL
                     PSLSYSVINDTWYKLFTEWFPDHGYELAENVQFFREKNDIHRYELWVPIIKGNNKRL"
     gene            complement(62417..63784)
                     /gene="rlmD"
                     /locus_tag="MJB72_000067"
     CDS             complement(62417..63784)
                     /gene="rlmD"
                     /locus_tag="MJB72_000067"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBY8261248.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHVECVVLM
                     SRVEK"
     gene            complement(63811..64533)
                     /locus_tag="MJB72_000068"
     CDS             complement(63811..64533)
                     /locus_tag="MJB72_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBY8261249.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(64533..64862)
                     /locus_tag="MJB72_000069"
     CDS             complement(64533..64862)
                     /locus_tag="MJB72_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBY8261250.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            65192..66118
                     /gene="fba"
                     /locus_tag="MJB72_000070"
     CDS             65192..66118
                     /gene="fba"
                     /locus_tag="MJB72_000070"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBY8261251.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(66253..67884)
                     /locus_tag="MJB72_000071"
     CDS             complement(66253..67884)
                     /locus_tag="MJB72_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261252.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(68367..69404)
                     /locus_tag="MJB72_000072"
     CDS             complement(68367..69404)
                     /locus_tag="MJB72_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY8261253.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(69423..70208)
                     /locus_tag="MJB72_000073"
     CDS             complement(69423..70208)
                     /locus_tag="MJB72_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY8261254.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(70241..71374)
                     /locus_tag="MJB72_000074"
     CDS             complement(70241..71374)
                     /locus_tag="MJB72_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBY8261255.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(71422..72549)
                     /gene="hadC"
                     /locus_tag="MJB72_000075"
     CDS             complement(71422..72549)
                     /gene="hadC"
                     /locus_tag="MJB72_000075"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBY8261256.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(72549..73775)
                     /gene="hadB"
                     /locus_tag="MJB72_000076"
     CDS             complement(72549..73775)
                     /gene="hadB"
                     /locus_tag="MJB72_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBY8261257.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(73790..74590)
                     /gene="hadI"
                     /locus_tag="MJB72_000077"
     CDS             complement(73790..74590)
                     /gene="hadI"
                     /locus_tag="MJB72_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBY8261258.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(74629..75828)
                     /gene="hadA"
                     /locus_tag="MJB72_000078"
     CDS             complement(74629..75828)
                     /gene="hadA"
                     /locus_tag="MJB72_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBY8261259.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
BASE COUNT        22122 a        16235 c        11913 g        25949 t
ORIGIN      
        1 tccattaaat attttaacag aattagaatc gcatataaaa gcaattggta tttgagcatt
       61 ccatataagc ttactaattt gttgtaattt tttttcttca aaagaagact ctataaataa
      121 tataaatggc ttactattta ctaaataaat tgcataagga tttataactt ttaaagtttt
      181 tacatcatgt ttagatatat ctaattttgt aagcatatct atattcacaa gattctttga
      241 ccctgaaaaa cctaaatttt ctataagctt ttctattggt aattttttca tagacattct
      301 ttctcctcac cttctataaa tagtaagtaa gtctcaatct caagcgcatc agcaattttc
      361 tgcacattat ctaaagcaat acttctcttt tttctttcaa tagcacaaat ataagttcta
      421 tgcaatccag ctttttcagc aaaagcttct tgtgatagac cttttttatt tctatatttt
      481 ctcacattgt tagcaaaaac tttcactatg tccattttca agaatcacct catattaaat
      541 atatcaaata gtatacaata agtaaacata ttaataagtc acctataaaa tttgctattt
      601 ctaattatgt atttaatttc caaaatgcag ttatatatat tagcattaca tgtaataact
      661 cgaaatagct ctacttgtct ttcaatatat attttaataa aacttattag aacccttcat
      721 catatactca taattttcta ttctctttta atccttacac acctattatt ttattaaata
      781 gctccaataa tatatcttta actcctgctg tattaaacac aagccctact gcaattaatg
      841 caactactaa aaatcctata agttttgaaa actctctctt aaatcctaaa tatacaccaa
      901 ttatagctat agccattaat accaatgatt gtgcatttga taaaaactaa ttatataaat
      961 tttgaccaaa attcattcgt atatctccct tcttttatat taaaatctgc aatactaatt
     1021 acattttttt attcatcata aataatgtct tcaacattta ctgaattttg ttcaattatt
     1081 tttttatgtt tatctgttag tcttgcatac cttattaagc cctcaattaa tgttgagttg
     1141 ttcaaactat ctaacctttt aattgttttt aacgttggtg caacttgtct tgataaccaa
     1201 tttaaagttt tttgaatatt atatggttct ggatttgttg ttagttttaa cttcgctcta
     1261 ccttcaccaa taaaataact ccactgatca ttagttttcc attcacttct aattttttca
     1321 tcatctttat caacaaaacg tatataacga ttaataattg aaaatgctgt cttttcttca
     1381 tctctatttg ctaacaaatc aactgctgca tgatatgctc tttcattttt taaccttatt
     1441 tcaaatctat tttttatttt tgcttcatca ataggtatat ctaattttat atattgctta
     1501 tagtcttttt catagataca aaagtatact tcacttttta atcaaccaat aatatcttct
     1561 atactagcaa tttcattaga acccgttata atttctttac tatcaccatc atactcaaat
     1621 ctaagttttg catttgctaa ttcctttcct gtgccttcat caatcttttt tactttaaca
     1681 attcctaact tgattacatt tatatttaaa tttgcactca tcgaactttc catatgaaat
     1741 tctgataagc tttgatagtt atgcttacga taaactattg atattccaac ctcattttta
     1801 ggaatcttta tataactaat aactcctgaa ttagaatttt tatttgctgt aattttcaaa
     1861 gtattttctt caagtaatat atctgttcca gtattatttg attctaattg catttctcta
     1921 ataataccat tcttatcttc taactcaatt gtttctccac ttttaactgc aactttttca
     1981 ttattccatg aaggtaatat attatgtcta tttaccttct caagtatttc agcttttctt
     2041 ttatgataat ttggaagtgt tgtagatata aatttatctc ctaactcctc cgatatatat
     2101 cctcctccac ttgaagtttt aattcctggc tctacacaaa atacaatttt attatccatc
     2161 ttcatacgat aaatgttatg attaattttt ttcctgtaat tggactaata cctaaataat
     2221 taaagcctgt ctgttcactc atatccaact cgctagcata agctgaaact gatgatgtaa
     2281 gtatagtagt aactatagtt ataaaaacca taataaaact tacaatcttt ttagatattt
     2341 ttgttttttt cttcatgcaa attcatcctt tctttaatat atttttatat aaaaaataga
     2401 catcaaatac ataaattcaa tgtctactta tataactaat atcttaaaat ataatatact
     2461 aaagttttta tcatcttaat caatactcta tcttttttac aatctcagta ttatcaaaca
     2521 attcgtagaa ttcattaata caataatgct tctgtgaatt cgaaaatcct gctggtctat
     2581 ctaagctaaa tgtaaaatca attcttcttt tagctcttga taatgcaaca aaaaatgcac
     2641 atctatcttc atctggttga tttttaaaat tccaaaatgc tgaatcttct aacccaacaa
     2701 aaaagatagt atcatattca agtcctttac ttttatgaat tgtcattatt ggaattgaat
     2761 ttttaccttc aaagttatca atcgctttaa tccaatcttt agtttcatta tattcaatcc
     2821 ataccagctt tttaaattta tctaccaaat catcaaaata atctttagat ttatattttt
     2881 gaaactttga tgcaaatttt tcatatgaaa tatattcaac cttagaatca actaaatctg
     2941 aaaattgttt ttcatcagta atatttaaat ttaccttgag atcattaatc attaattcta
     3001 ctttggtaat gaaatcattt aatattttag ggttataccc ttcgtctatt ccatataatt
     3061 cacctagaat atcgtaaata taatcccaat catcgggatt tctatcactt aacgacaacc
     3121 taaaaagcga aaccagtgtc tttattatat cttcttttaa tatctcctga tacattgctt
     3181 cattacgcgc cttaatatta cgttcagaaa gtttatttat aatctctttt gaataaatat
     3241 ctacgctctg ttttactaat atacatattt cattaatttc tatacctgtt tccaccaatt
     3301 cgactatttc ctcactaagt atatcagctt cttttatata atcctcaaaa agtcttaaat
     3361 acacttctcc atcttcataa ttccactttt cattagtttt taaatcaatt gcctcttcat
     3421 tcaaactgtt atacattgct ttttgaatct ctacaagtct tggcgctgaa cgatgattca
     3481 taattaatgt tcttttttga cttaaaaaat cattttgaaa atcttcaaat attgactttc
     3541 tagcaccagc ccataacata attcgttgtt tgccatcccc tacagcagta attattgttt
     3601 ctgttttaaa aaaacaagct ttaacaagat catattgaat agatttagta tcttgaaact
     3661 catctaaaaa cacatgacta taagtcattt caattgcttt cttaatatac ctattggttc
     3721 ttatcaaata ttcggctaaa cgtgatatca taccaaaagt aagtgaactt acaaaattag
     3781 tatctttgtt gcctttaagt agtacattcc aagcatctat aaaaatctta tctacaaaat
     3841 ctgtatgtaa aggcaattta tttatcgata gtttatttcc atatacttta ttaaattcag
     3901 tttgatttcc tctgaaattt aatcctgcta tctcaaatgc tcttctaaca tcacgatttt
     3961 cagctatatt gtaattattt aaaggtctat atgcttcatc taaactattt ctaaatctat
     4021 ctacgatttc ttttgcaaaa gcatcatagg tctttgattc aaatcttgaa gctaattctt
     4081 tcccacatct caattctaaa cgctctttta aattttcagc agcatccttt ttaaaactaa
     4141 tagcaaggat ttttttagga tatctacagg tattagtctc tagtaaaaag caggctctct
     4201 gagctaataa ctctgttttt ccagcgccag gaccagctac aacaagtgta ttcttatcat
     4261 taataactgt attcatcgct tcaacttcta gtctgaatcc agccgctgga ctccaatctt
     4321 ctttactaac ccaattcctc ttcattttct tccaccccct gaatcatcta tcatcttttc
     4381 aataatacta tttacaaact cctttagaca gtatggaaaa ctttcaaaat ctatatcagt
     4441 attattagaa aataaagata aatgagtagt tggtttacct cttcctaaaa agaaatatga
     4501 ataccatatc attagttctt tctctccctc agtataagta gatccatcac caccttcttg
     4561 ttttaaggtt gatttaatat cactatctat tctatcttta taagcagctt tcaatgattc
     4621 atcttcagca tcttcattga tcacaatatc ttgtattttt ccatgatctt ttatcctagg
     4681 accttccgca cttgttaatg ctgaaatata tttatcttta aaactttgta acattgcaaa
     4741 atctatatct aacggagctg aaaaataaac atcataacct tttagcattt caatccattt
     4801 agccatattt tcagtatcag ttacatccca agtatgcatc tctttaaatt cattatcttg
     4861 tgaaattcct tcacatgtat aaagtaatac attcttatca tgaccaatat ttattaattg
     4921 ttgtattgca tactttatac gtccccaacc acctccatct cgttctctat ctaaatctaa
     4981 caaagtaata aatggaattt ctaaatcatt taataaacgc caaaaatgat taacatgcct
     5041 accacctaat ggtactattg aaatttctga tgaatctaca gattctgctt ttaattcaat
     5101 catctgctta ataatgatct cttcactatc accttcgccc aatacaacaa gttttgcaaa
     5161 ataaagttca ggatatgcta atactgattc ctttacaaat ttaaattgtt ctgcttcctt
     5221 ttcttcattt ggtaatataa ttgacttcac ttttgtacat tcatattcct tacaaatcct
     5281 aaaatacctt atgtctgttg gttcaattct ttttactatt gctggtgaat gtgatgtcaa
     5341 tattgtctgt gaattttctt ttgtagaaat aaaatttaat ttttcaacta cccttcctaa
     5401 aagatgaggt gaaatatgat tttctggttc ttcaaccgca acaattgtta atacaggcgg
     5461 tattaatgta aatatatctt cttctttacc atcattaata gattttagaa tttcttcttc
     5521 aatttctaaa aatgtattta caagtgaaag ataaaataaa gaacgtaacc catctccaag
     5581 ctcttcaacc cgataatctc gatgtgtccc tgtaggtgaa aaagttactt caactttttt
     5641 taaaattgtc tctaaatcag tactattgaa gtttatcttt ggattagaat atctaatatc
     5701 cttatgataa ctattccatt gagtttcgag tgatttcttt aatgattcaa cccctttttc
     5761 ttcaaagaaa atttcctccg tttcacttgc tttcgtttta attcttgcct ttgtttgttc
     5821 tgaccaactt ataccattta ataatcttga aattattgtt cctgagacat tttttaactg
     5881 ttcatctggt ctcctaatgg caggtacata aatcatcttt atttgatcaa gctcatatct
     5941 tttgatatta cgcttatctt caggatttat ctcctctgtt gtagatgtta tatagtatac
     6001 atttgaatca atacttcctt caggatttga gcttttctcc caatttgctt caagtctaat
     6061 tcttaaataa ggtttcccac ctggttcact aacaaccata tttttaaaaa atactggtat
     6121 agatatgctg tctttacctc tctccaattc aggaaactca aattgtgctt caatatataa
     6181 ttcattctta gaaatactat ctggatcaat actttcaggt atatggaaat ctgatctttc
     6241 aataattcta tcactagact tttctccaaa aagtttcata agtgcactta ataaagcagt
     6301 tttaccactg ctattatgac ctataaatcc agttaaatta ttaatttcaa tagttgtagg
     6361 ttcttctcca aaacacctat agttactaag aattattttt gtcaattgca taataatttc
     6421 tctcccctaa gtccacttat ctatcttatg ttttttaatt ctgtattttt tcctttatta
     6481 tacatcttat gctaaaatat ttctattatt atacgaataa aaaataactc tattgtaaat
     6541 attttaaaaa tatatttaat tttttttatg aaatatcatt ctttagatta aatatatttt
     6601 cacataaaaa atagatatta aatttataaa tttaatatct atttctataa ttaaaacttt
     6661 ttttatttcg tattcccaca ctccatatat ataactacag acacatcaaa acaccttttc
     6721 aattctttat tcctaatttt attaccctct aatctaaata ttatatgtct atgaaactgt
     6781 ctaaagctca agatttctag cattattttc gacgtagcaa cactacagtc tcacaatggc
     6841 tcgagaggac agaatgaatg tcatataagt gtgtccgttc tcggaaacat atccactgcg
     6901 tttttggcac tatcggtaga cgggaacata tccaccaatc gtaccgttta cgaaaagggc
     6961 actcttacta ggagcgccct caataataaa cattaatgta atcaatagtc ttttttcttt
     7021 actggaatcc agatttcgct tcgatacttt ggatttccag tgtctggact ctcattccat
     7081 aaaatttcag gaccttcaac cgcctcgtat cctgaagatg gaaaccactc tgagtatatc
     7141 ctaccccaca cattttgaag tgtttccgga aatggtccaa tcgattcaaa tacagcccag
     7201 gtactagcat caatttttaa tacatcaaaa tctgcagttt cattacttga tgttgctacc
     7261 ccgatgtaat gatctaattc tcctttctct tccattctac cttctgaaaa attagtggaa
     7321 gcactaataa tacctgtagg ttctacattt gaaattgctt ttaattgatt aataacatcc
     7381 atggttaaaa gttcagtcat ttttgcaatc tctggattga caccttcaaa aataattgga
     7441 actctcttct taaatcctac taacttaaaa ggtcctttct caacaatacg atagttcatt
     7501 tcgcatcctc ctttaattga taattgaaag gtcattcgag gataagcttt taattgtgta
     7561 ttctcactcc ttgcctcaga agggagaatg ccatgcagag aatgaaaagc acgggaaaat
     7621 gaatcagccg aattatagcc atatttgacg gctacatcaa ttattctcaa atccctatct
     7681 ttcaaatcaa gtgcagccaa cgtcagtctt cttctccgaa tatattctga taaacttatg
     7741 cctgctaaaa aagaaaacat ccgcttgaaa tgatactctg aacaatatgc gattttagat
     7801 acttcaatat aatcaatttc ctctgttaag tgctcttcaa tatactccaa tgcattattc
     7861 atacttctta aagaatccat caaacaacct cctttcatca tcaatattat cagaggatgc
     7921 atttatccat ccgacaatct gtgcacagta ttgtcgattc ccttacatgt aattagtatc
     7981 gttaaactaa atttatcttt gggatgttta caacaggaat cctttacttt tcttcagtgt
     8041 atctttcttg ctgcaaaaat tcctttgctt cgctaaggca taaatcccaa tgactctttc
     8101 ttcaaagtca gcttcatcta gccttgcatt gctatcttct cttattcgtt ccaacagttc
     8161 acttccaaat ccagaaaggg aatctttaat agaacgtgcc gtatcttttc ctcctctaaa
     8221 ttctaattaa atcggaattt gtcagttact tgtattcact cggtacagga atattaaatc
     8281 tctcgcacaa tagttttaca tcatgcatat cattttcatc atgctcatag cccagatgaa
     8341 ataacacttg attttcagca tcgatacatc ttaccgtttt atcgcctatt ttcccaatgc
     8401 cgctaaagac ttctggagga tatgcttctc cctcaaaaac aatgtatcct tgttcgttga
     8461 atttaaatat atgaagatca attatcctac ctttactatc cttataaacc gtatgagatg
     8521 tggtggtata tgcttcgata acttcagcaa agccatattc ttttaatatt tcaataaact
     8581 ttttaccatt actttcttcc acaaataaat caatatcgtt gtgtactctt gtttcttctt
     8641 ctaatagagc atctactccc caaccaccat ctatccaaat gttaatatca ttttcttcag
     8701 catatgttat tatctcaatg gcatcagttt tacttaccat gtaatccctc cgttcaaatt
     8761 aaatctataa gtgataaatc tcaaagctat ttctcctaat aatataatgt ctattgttcc
     8821 aatcaaagct aaagatgaag cttgccttct ttagctcgca ataattagag ctaaatcgag
     8881 attccagcac cccctagatt taagttccct tttgtgccgt ggtagaaagg ccccaagtct
     8941 aggaattttt catgaattgc tctaaccctg ctttggatgt taacatttca agattattaa
     9001 atatttcctc ttcgctccag ttccaccatt gtagctttag caagaattca atcttttcgt
     9061 cactaaagcg ttttttgata aacttagctg gattcccacc atatattgta tatggctcaa
     9121 cacttttcac tactgttgaa ttagcggcaa taatcgcacc atcaccaatt ttaatacccg
     9181 gcataatggt aacattttga cctatccata catcattgcc aatcactgta tctcccttaa
     9241 aaggaagttg ttcaattgta ggagtcacct tttcccatcc atggccaaag atattaaagg
     9301 gataggttgt aattccatcc attctatgat ttgcaccatt cataataaac ttaacaccct
     9361 ctgcaattgc acagaacttt cctattataa gtttatcccc tagaaattcg taatggtgct
     9421 ctatattatc ataaaatttc tccggtgttt ttttattatc gctataatag gtatattccc
     9481 caatctccac attgggtctt ttaggtagat tgcttataaa acaaaccgtc tttatatttt
     9541 catttggata aagttttttc ttatctggtc ctatcataaa atattagctc ctttaatctt
     9601 taattacttc attacctttt atcttaaacg cattgaggct gtcatagttt cggttcctcc
     9661 tgaattctta tttgtatcag cgtatatttt atcacaataa ggttctatat tcaacacaaa
     9721 aaatataaag gaaggaaata ccgccaaagg cgatagtttc cttaattgcg gttgactcaa
     9781 taatctatat catttcttca atcttcacac ctgacttaaa ctccacctca atcctgtcat
     9841 cgcggatcgt aactttttca ataagccgcc ttaccaactg ctcatcatac tcctctaact
     9901 cgtgagactg ttcatttaag aaagcagtca tttcagcgat tcgttgcctt tttccttcac
     9961 gctcggcatt ttctacaagt gaattttgct tcatctcccg aaggcggtat atctcatcag
    10021 caacattatc gtagtcattt tttgactttg cctgtttaag aagctctatc tgcaattctt
    10081 ccaacttgct atcaagctca tccgtatcat tatcactttc ttcattaaat atagtgtcga
    10141 tatttttctg taatgttgaa aggaaaggtt ctttgttagc caaaagttcg ttaatagccc
    10201 tgaccactgc tgtctgtaat gtttcctcgt ttatggtagg ggcagtgcat tcagaccctt
    10261 tttcctccaa tcggctaacg catctccaga caatagactt atagcctcgg ttattccaat
    10321 gtacccgtcg gtaaatatcg ccgcaatgac cgcaataaac aatactcgat aaagcatact
    10381 tgctgctata aactctcttt ttaccgcctt tcccgccacg aagattcgct ctccgaacca
    10441 tctcttcttg aacctgcata aaaagctcac gtggaatgat aggctcatgg ctgttttcca
    10501 cataatactg gggaacaatg ccgttattct tgactcgttt tttagaaagg aaatcaaccg
    10561 tatatgtttt ttgtagaagg gcatcaccga tgtacttttc attctgcaat atctttttca
    10621 gtgtttctgg tctccatttg gctttgcctg ccgctgtaag aataccgtct gtttccagcc
    10681 ctcttgctat ctgtaaaagg cttgctccct caaggtactc tctgtaaatc cgtttaacaa
    10741 cctcagcacc ctttggttca atcactagct gcttattttc atccttggtg tatccaagga
    10801 aacgcttgtg gttgacctgt acttcacctt gttgatagcg atactgaata cctagcttaa
    10861 cgttctggct taaggattgg ctttcttgtt gggcaaggga tgccataatg gtcagcaaga
    10921 tttccccctt ggaatccatg gtgttgatat tctctttctc gaagaacaca gcaacgtttt
    10981 tatcctttaa ttgacggatg tatttaaggc agtccaacgt gtttctagcg aatcggctga
    11041 tggattttgt gataatcata tcaatattgc ccgccataca ctcttcaatc atgcggttga
    11101 attcatcacg ctttttggta tttgtacctg tgataccgtc atccgcaaaa attcctgcca
    11161 attcccattc cttgttcttc ttaatataat ttgtataatg ttcaatctga atgtcatagc
    11221 ttgaagcttg ctcctcacta tccgttgaaa cacgacagta agcagccact cgtatttttg
    11281 atttgctttc actattttta ttatttccaa cccgtttaat tgccggaatc actgttacat
    11341 tcctacttac cgccacttgt tagacctcac tttctatcaa actgtaggca tattctgcct
    11401 gcttatatgg atcttcatat ttttgcacca gaggttttgc tttgaacttt acagggtaat
    11461 ccctttccgg ttcatcttta ggctcccata tccttccgag cttttctgct cgttttcgtt
    11521 tttctactct ggctttttca aaggtctctt catcaataat tggagggtag aattcatcgc
    11581 ccaagtaatg cttgttctgc aacatcttac ttgctgtggc atggtagcag tctagcccag
    11641 cttttttagc agcgcccttc aaagaaagtc ctgctaagta ccctgaaaat aattctctta
    11701 ctttttctgc tgctatatca tccacaacag cctttccatc ttcaattcta tatccatagg
    11761 gtgtgtgacc catctaattc accaacctct ccttcaatgt gattccacat tttaattcaa
    11821 atccgacttc ctctcgtgaa aaaacaataa tcttttctac gtaattttca aacagctcat
    11881 cctcataggc tgtgagcatt ttggacttag tggcaaactt aagtaaacgg tcaacttcgt
    11941 ctacttttgc aaaattgcca ttgacggaac gagtaagttg atccttttca gcaagaagcc
    12001 tttctctttc tgcttccagt gaattctttt ctttattaaa cagggcaggt tccagatacc
    12061 ctttggccat taaatccgtc agcatctggc tctgctccat gttgttttca atcttagttt
    12121 ccaattcttc aattctgcga aaacttgccg cattgttctg gttacgtaac ccattcaaaa
    12181 gtggtcttaa tatgaatttc tgaccgaaaa tgagtttatt catcatcgtg acaaatgcag
    12241 tctttgtatc atcatctcgt atgaactgca tagaacattc cgttatattg cttatatgct
    12301 tactgcagca ccaagcaatg tattttcttg gaccggatga atgaatccgt cttttaaagg
    12361 tactgccaca ttccgagcag ataattttgc tagagaaagc atatcggttt agatatttgc
    12421 tgttgcgctt ttcgatgcct ttttcttttg ctctctgatt aagaatggca tctacagctt
    12481 caaaatcctc atagctgata attgcctcat ggtggttttc tactagatac atatttttct
    12541 caccataatt ggtgtgcctg ttaaaatggc tgtcagtata agtcttttgc aaaataacgt
    12601 cgccagtata tttttcattg gtcagaatcc ctcgaatggt agtagccgtc caacggccac
    12661 ctctttttga tgggataccc ttttgattaa gattatttgc aactttctgt gtacctttgc
    12721 ccgataatac ttctgcaaaa atatacttta caatttcagc ctgcttaggg attactttca
    12781 tttgaccatc aatgttttga tacccatatg gtgggtagga aattataaag gttccgtttt
    12841 gaaatcgtct ttgaatggcc cacttcgtat tctctgaaat ggaaattgac tcactttctg
    12901 caagcccact taaaatggag agcatcaatt cactttccat tgaacctgta ttgatgtttt
    12961 ccttctcaaa atagatatga accccaaggt ctatcagttt tcgaaccatc tccaagcagt
    13021 ctgtagtatt tctcgcaaat cggctgatgg actttgtaat gattaagtca attctcccag
    13081 tttcacagtc tgataacatt ctaagtaggt cagagcggtt ttcctttttc gtgccgctga
    13141 taccctcgtc ataatataag cctgcgtatt cccattctgg atttgccttt atgtagctct
    13201 cataatgggc cttttgtgct tgcaggctga caagttgttc atcactgcct gttgaaactc
    13261 ggcaataggc aactactcgt gtttttggct taataaaaga gttggccgta tttccttcta
    13321 ttttcgttat ctttttcatc ctctcacctc cttcttggta ggtcacatat tacctctgaa
    13381 acccttatat atcaacgatt cgagggcatt atctccttca aaaatggaga gaaagtttgg
    13441 cgatttagtg cgcctatttt gttgaattcc acttccgtta ttaagccttt ttcgagcatc
    13501 ttactcagca acttttctgc cctataataa tcaaattctc tttgaagcgc ctcagcactt
    13561 aaaagtgtct ttttagaaat gcttatttga tcttgtttat ctgtaatttt agttatctgc
    13621 atataggcaa cctccatttc tacagtgaaa accctgcacc tatatgcaaa aatccctaat
    13681 gattcgaacc cctaaaggtc aaaaaaaaat gacccgaaga gctattacac tcctcaggtc
    13741 ataggtaaaa gtgttaatca tactttataa acgaatccgt aaagccagcc tttttcgctt
    13801 tggcaagctg tgcctctgca ttggctttta cagaataggc accgatttgc acacgataat
    13861 atttcttttt ctcctgttct gcaggcttct ctccttcact taatagcttt ttcacatccg
    13921 ctctgaagat atccatgctt tttccatgct ttggaaacca gtttttcgga tcgccatgat
    13981 tacttgcgat gcctttttga tatccttcat agtggccaat aacatctttt tcggttaagt
    14041 tatagagctt gcagagatac acacacagtt ctaccgcttc cttataaacc gcattaaaat
    14101 acgaggcatc ggtcaaaccg tcctcgcaga tttcaaaacc aatatgagta tcatttgcct
    14161 ttccacctgc atgccagcca cgatggttcc atggcagggt ttgataagtg gcaattgaac
    14221 catccttaag ctttccaatg aatgcatgaa cacatacttg cctcccacct ggtttatcct
    14281 gattccagtg attgttgtac tcgtttacac ccaacaggcc atcgtccggt ccaacgtatc
    14341 tacgaagata cggattattt gcaccagtgc tatgaaccat gatgcccttc ggtataatct
    14401 ttctgcctgc cttataacac gcattttcag ttagaataag tttcctcaga ttcattactc
    14461 atttcctcct ttgttatgca actgtacaag aatgtctttt aacttttctg gtattggcaa
    14521 tcctagtcga ccagcatttt ctaacataga tattccttca ttggagcaat aaaaaaagat
    14581 aatggctgtc cggagcacgc tcccctctcc gataaggttc gtatcaatca tatgtccgat
    14641 tccgactaaa acaaaaataa gcaccttctt aaagatgccc ctaaatccga tctcactaga
    14701 tagcttctta tcaacaatgg cacacatgac accagtgata taatcagcta tcgtcaatgc
    14761 cactaatgca tataaaaaac catcaaaacc acctaaaaac cagcccaaga atccaccaag
    14821 tgctgtaagg gcaacttgta ccccattcca tatttccttc atgattttta cctccttcgt
    14881 gattggtgaa tatataaaaa gagtgcttgc aatttttcgc aaacactcct gaatcgttaa
    14941 ttatatttgt ttaggcagtg cctcccatag cctcatatcc tcttggccta aagaccatat
    15001 ggcaattcct ctaagcttcc atcgatacgc tgcctcgttt gcccaataaa caaggctgtc
    15061 tacatcctga taataaagaa tagaaaaacc gtcagcatct ccaagaaaca aacgtgaaat
    15121 ccagacattg atgtccttgg gaacgatttt tactgaataa tcatttccac agactaatgg
    15181 caaaagacct gaatggaaga aatcataatc catagagatg tcctggtttc gtgtagaagc
    15241 ttcctccaca tcattgttaa ctgtgaaaac ctgaaactca ctatcccatg ttacaccggt
    15301 tcttgccagc cgcccatact cagttgtggt tccatccggg aaggttacat caaacctttc
    15361 ataaggttca taagtccagg catctcccag tctaagtaat tcacatagaa tacgtccatc
    15421 agaccgaacc ccggcatacc ctcctgaaaa accactaagc gtcgctgtaa agcgcaaaac
    15481 attgcttgct ccagaataaa ccctcacaga gttacctcgt atcctcattt caatggtgta
    15541 cattcttgga tttgagcgaa gatctgctga tgccgtttta acgatttcag tggtgtaact
    15601 gccaattaaa gaagcaccat tatatagttc aatacgctgt gtattaaagt ttaaacagca
    15661 aaagacatta ccacaaaaaa tgcctgcacg accacttccc tctggagtaa aggccaacct
    15721 tgcccgcaaa tgaacatctg aaaacccact atacttccac gccaattggc catacccgtc
    15781 aagctgcgaa tagggacgac tggcgggatc attttgattt tgccatacct gccactcgcc
    15841 gcttagtgct gtccagtagc tggagggaag tggattgtca tctctgaagt cttcatacca
    15901 aattagtgct gaatcaggct ttctgcgtag cacctcagtg gtcaatttaa aacctttatc
    15961 gggttcagcc attaaaccgt tcacatcttt aaatttgcga ggagatagca taaactctgc
    16021 ctctccagca gaaggttctt cagaaaaact cgaacatact cgaaatccat agaactgcac
    16081 acctggtact gaggtactaa cgctgacagt atgggtacca gctgaaagaa atacaccaga
    16141 aaccaaggaa agccaacaag ttgttcgcca atatggccac cacagcctat tctcgctaaa
    16201 taccttactt gtcccgtcaa gtgaaacatg aatgctattc ttatcccaga agggaaatga
    16261 gatgcgaatg gctacatcat aaataccagc agatgaaatt tcaaattcgt attcggcttc
    16321 accttcttca ccaagtgtta tcatttgaga agaaaccgat acattaccag tatagctatc
    16381 aggagttccg ccactacgat ctacatatat cgtgccaaat tcagtttttt gctgcttgct
    16441 ataggcagtc aaatacctgc gacggttata agtttcaccc agtaaaggat aggttcttga
    16501 aactgcatcc cagccttcca tgtagtcata cacatgtggc aaggcccaag gcaccttatc
    16561 ataatcatcc caatacgcta tgataggaat catgggctgt ggtggtccat catccgtaaa
    16621 gttatatccc cctgtcatcc aaagctgtgc cgcataatat gtgtttgaaa tcccacgata
    16681 agtgatgccc agattttctg gtgtatcgtg gattctccag ttccaaccat atgcaggcag
    16741 tcctaaaaat attttttcag gagtcataac ccggactgca taatcataaa tgccttccaa
    16801 ccaatccctg ggagataccg aacccggtgc agagcctgcc cacgccatgc cataactcat
    16861 aatcgctgct gtatcacaat aggcattcaa gtcttcataa acacaccagt tttcgccacc
    16921 aaccgagcct tgtactccag tcatgcccgg tagacaaata tttacgagtt tcgaagaatc
    16981 ataggcttta acagagttat atatgtctcg aaatagagca ttagcagcat ccttgttttc
    17041 atagccacct ccacgttcca agtctatatc cactccagca caccaaggat acttattcat
    17101 gatgcgaata atctcagtaa gaaatttatc ctttgcacca ttggtgttat ttctaagggc
    17161 tgtaaaaata gaagccgtac catggttcat gattgtaagc agccatttga tatgaggcca
    17221 gcggttaatg taagtaagca tactggatat gcttgttcct gtttctgtta tggtcccggt
    17281 gatatccact tcaaaagtaa aaatacccac cgtatcaatt cgatcaccat agtctcgtaa
    17341 tgcttgatac atgcgggtat tccccatgaa actccagacc atacaacgct taccttttaa
    17401 atagtcaatc aaaggcgctc acttccttcc tgcatttctt gaaactcaaa taatacccgg
    17461 gctgattttc tgtcttctag ctttatcaca tgtttgctat caccggatgc tgtatattga
    17521 aaaaaacctt ccttatcagt tgggtttccg ttctttaaac actgcctagt tgatgctagt
    17581 agcgaaaagg tatcacctgg actaactgcc tcattaaata tcaccttgtg cgcccctgct
    17641 ccttgagaca attggatact gcctgcagcc atattttggt tgggataaat atgacagtca
    17701 aggccagcgg aagttgaacc cagattgaag agaataaccg tctctccgct acgaaccact
    17761 ccgttataaa aacgagcggg aacaataaca ccgccctccc ggaatttttg aagcatcgtt
    17821 tctgtattaa ttgtataacc agttagacga tcaccttcct gagcctgaat atcagtgaaa
    17881 taaacagtac ctgtgcaatc tccaagaagt agtttcactg ttacgcttac tactcgttta
    17941 tcctctttac aatgaatcgt ctcagcaaac ctggtaaatg ttactgacat ctgcaccacc
    18001 tacccatcca acgtccactt aatttctgac acatgaccaa tccagcccgt agcaacagaa
    18061 ccaccttgca aaagcatatc tgtaaagaac acctcaccag aacaatcagt aatgaaaaga
    18121 cggatggtaa gcgactttat ctttccaata cctctcggtg taatagcatg tgctgtctgt
    18181 gaaaaataag ccatagcact ggctccttcc taaaagagat caataaacct ggtttctgtc
    18241 gacccatcct catattcaat gacaacttca atcccaactt gcccgtttgg ccctttctga
    18301 agattttccg atgcaatctg cgctgagaaa gtgtaactct tcctactggc aggataaacg
    18361 gtctgggata gacttttcgt catccctaaa acgccctcag ctttaaaaga agctgttcct
    18421 gatacaccat tattagggtc aactgtaaaa cctgaactta accaataggt tagaccatca
    18481 tcagctcgtg aatttcgtag atgattaaaa ggcactaagt cttttaattc ctgacggtca
    18541 agaacatcag tggaagataa aatatcggct gctttatccc atccagcaga ggaatctcct
    18601 aattctctta aagttgttga tagctccagc actgttttcc atggttcttg tagattatat
    18661 tgtctgcgaa ctacacgagt tttgacagat aacttcaaat ctctatcatc cacagttact
    18721 atatcaccta gtttccatgc ctcatgctca tatcctgtca acactgataa gtccattgca
    18781 gagagaacat atgagattcg tggcttcgca tactgagcta agcgcatatt agcgaactca
    18841 agcagctgat aagggttgct gattgatgaa gcatccagcg ttccaactct aacttctgat
    18901 gaatagctat aatcctcaac atactcttta ttaccattga tagaagcaaa tgttatacca
    18961 tcttttccat aagcataaag gcgagtgatt agacttctag tatccactac gcgctgtata
    19021 cttttcatgt tctttctata gcaaaataat gccccactat cagtacctcc aaatgtcaaa
    19081 aggtgtacca gacgattggc actatcgaaa atcaagtcac caccatgaat attctgtatg
    19141 gctcgaagaa tagataaagc attcttctct gttgattgcc atgttcgttt tgtactgaca
    19201 gtgacatttc ctaatgacca gccagtacca agtaaggcat agcgcatagg tacttctggt
    19261 gtctctgcga caaattccat gggttctttt tcagcgctaa aggaaagatc ataaaatgca
    19321 gcttccgcat atacctgagt tacaactctt ccatctgagc ttttctcatc tgttatggtt
    19381 cgaatacgat aaacatcatt gacaatctgc acctgttttt cattgtctaa ggtctcgcgc
    19441 ttggaatcat gaaatggcag tttaaactcc aaaatatcag ctccattcac ctcacttgtt
    19501 accacaatat caaaggcatt ttctaacacc gcttcccaag ccccgttagt atccaacaca
    19561 actggtctgg caaatccaag tctctcataa ggaggttttg gtatatcatg tagttgtatt
    19621 tcaagaagtt taggagttct actggtgtct tgagtagata atgtaattcg ataacggata
    19681 tattctctgt taggcgactg cagttcccca cttgctccaa cggcctgcca ttcagtccag
    19741 ctagacagat cgtctgatgt agaagtttcc actaggctta tggctgttat acctgcggtg
    19801 tattcacttg taactgatac cctgcccgtt cctgcaagac tacaggcagc tgctatcgta
    19861 aaaagctgac cactttgtgc atagactccg tttgttgctt tcaacatgac agagccaggg
    19921 tccgcaagag catctacatc agcagcactg tcaccaccgt ttgccaaaat tgaagatttg
    19981 aaataggaaa taagatcctc catggtaagt aaagaatctt tttcataaaa ccagtcatca
    20041 aatcctcctg cgtaataata ggtatttgca tgcatcccca tcacaatatc cgcaacacaa
    20101 gattgattta agtctccagt gaatgttcga actggagatt gccaaacagc gccatcactt
    20161 cggtcacaaa gtaagttctg cacccttttg ttgtttacct cgataacaga agcgataaag
    20221 taccacccgt tattctttag ggtaatagta ggcgtttcgg tctgatcata tatcaaggag
    20281 cctgaggcat tatacagcat taatcgcaac cttccctgaa ataaagaaac ataaaaaatt
    20341 ggctgaccag gcccttgtct agtattaaaa atcgggatat acgtctgccc tacagaataa
    20401 gtagttggat ttatccatcc gccaacaaca atcttttcac ctaaatcact aaaaaagcta
    20461 ccgtcattgg tagccactaa atgagtcttt tcacttgttg gattaatgat attctgcctg
    20521 aaatatcttc ctaatctacc ggataacaag ttggctgatg tgcctgacca tccggataca
    20581 aagaagtttc tgttatgacc ggagtcatct gtaagcatat tgttgctatc tggtgcggct
    20641 tcattaaatc gccacagagc aactgtcttc tcgcttacag gaaactcacc cgtaaaatcc
    20701 gtctgtgatg ttaagattga tttaattgcc accagatcac ctccatctgc ttttggccag
    20761 aatcttcagc tccgaaaatg ttgctcccac agctgatact gttatttcat tttcaccttt
    20821 gtgaagtaca gggaagttta actcatccag gactggaagc ccatttcgaa gtgtatttcc
    20881 gttggcatcg gttatttttg ctgtcactaa tccgctatca attacaagca cttcatctgc
    20941 cgtcaaagct ccaactactc gaagttcctc tccattcgtg gtaatagaaa tatatgtgga
    21001 tgtagatcca cttattgatc ccttaagttg atatatcggt tctgaatctg tattcccggt
    21061 tattctttgg atctcatgag ttccaacact tgaaatcgtg aattgttcat ctgtcagcgc
    21121 ataggcgtga ggatcaggac agataaactt caactcaaag gcacctgcag ttctaagtaa
    21181 tctctcgcat tctacttgtt cagtgaggcg agcaaaaaag aatcgattcg gaatatcctc
    21241 taatataagc tgcttaaggc cgttcatagg gttgagccac tctgcaagat catctaacac
    21301 gctcactaaa tctgcaaagc tcttttgagg atatatgcta cagctaatcg taatgatccg
    21361 ctctgagata tcacaaccaa agtcagcaac ccctgccttc cccggaacaa cctcatacgc
    21421 gtttcttaat gcgggcgatg cctgccaact tgtgagcctt gcttttatct tcatattctt
    21481 tgagttaata ccgttataaa taaagcccat acatcgccct cctttacgca gttataaatc
    21541 ttccttgtgc tcgagatcct gtctgcatca aattgtataa ctcctgtgaa atcttccgaa
    21601 tatcgtcttc acttcgaaca atcatctgct gtatcgtaat taatgaccct ccaaatgttc
    21661 catatccgct actaataccg gctccattca tattcagatt agggttaatg tcaaaatcag
    21721 tagggatagc gttttgcata tcctggctaa cactattcat ggctttttca aaaccgacac
    21781 caataccctc tcccatgttt tcgccaattc cagcaaacaa tgtagatggc gatcgaattc
    21841 caaagaaatt ttttatttta tctactacac caccaaaaaa gcctgagatt ttatcccaaa
    21901 gccaggctcc agcatctgaa ataccctgcc acaaaccttt gattaaattg gtcccaacct
    21961 gggcaatttg ccaaatagat cctgtaaaac ctttaaccag tgccgcaata atttgtggca
    22021 ctgctttaac aacctctact ataatggctg gaaggttctt aatcagcgaa accaacagca
    22081 tgattcctgc ttgaatgatc tgtggtatgc tgccaataat agcattcaca agggaggata
    22141 caatctttgg tatagcagta ataactgttg taataattag tggtagattt tgaatcagtg
    22201 ccactagcag ttgaacccca gcatcaatga gcagtgggat tgagcccaaa atcgctgtga
    22261 ttaacccgtc aacaatctga ggaattgctt ctacaatggc ggtaatgatt tctggcaaag
    22321 caccaattaa agatgtcaga agttgaatac cagcctcgat tatttgaggt attgcgccta
    22381 taacaaaatt tactatgcca gtaataatgg ctggcaaagc cgcgatcagc actggaaggg
    22441 catctaaaat accttgagtt aatccaagta caagttgcag agctgcttct aaaaccatag
    22501 gaaggttatc caaaagcccc tgtacgattt gtaccaccgc tgaaactgca gcaggaatta
    22561 actgtggcag agcttctgca attccactta ttatggttcc aatcatttga atacctgctg
    22621 taattagcgc tggaagtgtt tcaatgattc cattgactaa tgtcatcagt agcatgactg
    22681 cagcttctgt tatttgcggt aatgcagtgg taagcccttg aaccagagaa ataataatct
    22741 gcgaagcaat atctactaca attggcaact gctcagatat aaactgaact gcttcctcta
    22801 agattcctcc aaaagcatca attaagcctt gaacaccgtc tttttcaaat gctccagaca
    22861 attcatcgac ccaaccatta accatgggaa gaacggtacc ggaaagcata gttgttaagc
    22921 cttcagcaag ttggcccttc aatgaagcaa caccatcttc gagagttgcc atctgcccag
    22981 aaaaggtttt tgactgagct tccattgacc catagaaacg cccaccttcc gatgtagctg
    23041 aggcaaacgc ttctgcgacc atatctgcag atattgcccc tttggacatt tcatccttaa
    23101 gttcaccaat ggattttcct gtcttacgcg agatctcctc tagtgggtta aatcctgcat
    23161 taatcatttg catcaagtcc tgtccggtta acttaccagt agaggacatt tgtgcaaatg
    23221 ccagtgtcag gcttttgaac ttctcggcat ctccctgaga tatatcaccc agctgcttca
    23281 tgcgtttttg agcttcttct gcagacatac caaagctcat aagggtttga gctgattggg
    23341 caagatcctg cattccaaat ggtgtagctg ctgcttcttt tttcagatca ttaaccagct
    23401 tttgagcttt agcctcatca ccaagcatgg tggtaaagga agctgtgtag ttttccattt
    23461 gagcgttgta ctcaacgcca tctttcatag cgcctataaa ggcttttcca attcccgcaa
    23521 tggctgagcc taaagctttt accccaccaa ttatggcttc tgataacaaa tttgctttaa
    23581 gtacatctcc aaaaacagag gttttcttgc tagcatcatc catttcatcg ccaacatcat
    23641 caacattatc tgcaagttca ttggctgcat cagcggcatc ttccatgtta tcagcactat
    23701 catctgtggc gtcggcatgg tctcgtagcg ctttatggtt attttcaagt tcccgctcca
    23761 tgccattaag ttcagccaat gctttattta gttgaatctg ccagttttgg gttctacgat
    23821 cgttttctcc aaaagaagta gaggcgttat ctagggcagc acgtagtgtc tctatttttt
    23881 ctttttgtgc atcaatggat ttttctaata cttcatttct tgcagttaag gcttggatgg
    23941 atttatcgtt tttatcaaac tgggaactga ctagagccat ctcactacct aatactttaa
    24001 atgcttggtt aatgtcacga agtgcatttt tgaattcctt ttcgccttcc actccgattt
    24061 ttaaaccaaa attatctgcc aagattaccg cctcctttct tttgaaagat caaataccat
    24121 aaggaataat gtcatcgatg taaatcttcc gttttggttt ggacattcca agaaactgct
    24181 tatggcactc ccataaatcc ataagcaagc caataggtgt aagccatgtt tcctcttcgg
    24241 tacgatttaa atgaactgtc ccgtaatata aaagccgggt aaagagttca ttctcgttta
    24301 cccggtttaa acgttttttg agtcatcttc agattctaca tttctcttgg ttcccttaaa
    24361 cattgcttct gtcaaagcat tcttataagt agctagttcc aaaggagatg tgagaagttc
    24421 aacttcttct tgagtaagca gcggcttttt gttatctgga tttcgaaggt tgtgaatgag
    24481 taggctctga ttggccatta aagttatcag ccagactatt tcatccagtg ccatttcgaa
    24541 gttctcagac ttcagtaatt tctcacccaa attttcaaga ccgccatatc tcccagcaat
    24601 ttcttttgta gctttagtgg taagaataag ctcatactcc tgcccgccaa tattgatctt
    24661 tgcacttctt tcgttatcca tttcaattcc cccttactct ccaattccac tagaagctgc
    24721 gaatgtgggc tcatacactt cgttgaacca gctattaata accgttgaag ttaccccttc
    24781 atcaccctcg ttaacttccg ccttccatgg atgcttaccc tgaccatcta gcttatttct
    24841 tcgaagcact gttccttcga tagttggggt tgaaaaagtg atactatcgc ctttggtcgc
    24901 caagtttgtt gcaggaattc caaacttcac acgatacagc caaaagtatc ggtatttacc
    24961 gtttgctttt tgtgctctaa agccaattgc aacaggagcc cctccatcct cactggttga
    25021 aatgagtaca tggttgtcat caatggttgc tcctgttaaa tctcctgcag ctgctacccc
    25081 tatatcatca ataccgagag ttagggtacc acttttaaat tctttgacaa tttcagcggc
    25141 accatcatcg gcataaagag tggcctcagc aagctcaacg gaaagttctg cactgattgc
    25201 ttttgccagc ggcatcggtg tatcataggt ttcatcaccg tttatgtctt cggtgatttt
    25261 tgcataataa agtctatcaa gaccgattgt agccatgttt tattcctcct tttccaattg
    25321 aaattcatat ggtttagcca catctatggc ataatgatga taatcggtat catcctcatg
    25381 tccgatgtat cgacgatccg ttatcgtaaa atccgcacct agaagagtgc ggacaattgc
    25441 attttttata gctgtataac tacctttaac aaataaggaa agtctggctt cctgtacttc
    25501 atatcccggt gtgttatccg catgaacctc aaacaaatca ataagaggtg taatcacaag
    25561 ataagtatca ggaggaacac cagaaaatct tcctgtttct actgggatac cgcacaaatc
    25621 tgctatgaga ttaagctcat ttaatatact cataagtttt cgacctcctg ctcaaacctc
    25681 ttcttcatag cgtcaataca tgcttttctt gaagcccttc ttgcaggctt taaaaatggt
    25741 tttggtggct gacccgattt tccgtattcg atgatattgg caatcttagc gttactttcg
    25801 ccgcttcttc gtggctcctt aaaacctatc ttcacgttga aattgccatt tcggtccact
    25861 ttagcaggag agagacccaa cgaactaata agctcacctg tagaccgact tttttctttt
    25921 gttccactac caactatagc ttgcaaatta gatctaacct tttccaaaac gatttccccg
    25981 cctgactcaa gtacctttgg aatgatttca tccgtcttat cgccaagctt tgaaagcttt
    26041 aaaagaaaat cctcaggcat tttcacttgt accttagcca cttgacccca ccaccttttt
    26101 tgccatggct tcaatgtaca tgcctcgacc ttttacatct tcaacatttg taatctcata
    26161 gcggccatca ttacacacaa tcaccatatt ggtagatacc tcaacatcag gtatcttacg
    26221 aaaacgaaac agcgcagttg cttcagaaaa tgtcgctcgg ttagcccact tttcatttcc
    26281 atgacgatct tccttataag cacgaacgga agcgaggatg gtgtccttag atttaccgaa
    26341 gccttcactg tctttcgttc tttcaacaga aatgagatcg ataaaggtat tcatttttcc
    26401 aaaactcata ctctacacct tccaatcccg gtcaagcctg agcagtaaat ttacagtgtt
    26461 ccaaacctgc tggcctgcct gcacgttatc agcaaaaaag ccgccagtgc taccatccct
    26521 actttcatag aagtgagatg acaacataat gacggcctgc tcggtagttg gcggcattgt
    26581 gttctcatta taatgacctt ccggtacatg ctggtaactc tcggcatatg cgatagcggt
    26641 ggtgatgtac atttgaagaa gttcatcatc gcgatcgtgc tcaagaatta gatttgcttt
    26701 aactttttct aaaagtgtca tcaccatcac tatcctttct aaggtgtatc tccattcatg
    26761 attcctgcag ttttcagctt actgagcaag gcattaaagt ccgtcactag agcttctaca
    26821 gtttccgcag tgcttgccgg ttgattttca agaacaggga gcccagttac tttggctccc
    26881 tcttcaatga caagctctcc accaataacg gttttttctc caccttgttc ggtgtagttc
    26941 tttgcgctat agctcataat ttgcacctcc cattaagcct tctgctgaag aactttgatg
    27001 gcttcaggta gaatgagctt tccatctaca cgctggcttg caaggaaacc aacctgccca
    27061 gtagttgcaa aaagctcgtt taaacgtttg aaagaacgcc cctgtctgtc agcaatccaa
    27121 tagtatccga aatcaccgaa agcaatcgtc tttgctccgg cttcaataat aggagcataa
    27181 gcagaagtgt aaaccggacg attcagcaac gtatctggag tacctgctgt taaagaaggc
    27241 tgccatagat attggccttg accatctttt agcttacgaa ttgcttttac agttgcatca
    27301 ttcatcagga ataccgcatt ctttctatat ggtgctttta atgagtacac aaggtctata
    27361 atttcatctg cagtaatagc tgttgcagat cctgctgaca ctccaagttg tgcaccacca
    27421 gttacattga aaattcctgt aggttttcca tctccgtcac caacaagaaa tgcttcttct
    27481 tcctttgcac cgattcttcg agcaaattca gtggaaatat atttctctaa atcaaatacg
    27541 ctgtcattaa gaagttcatc agaaacctta atcattgtac ccagcttata ggcacctatt
    27601 gatgtctgac cgaatacaga atcactctca tcaaactcct caccttcgtc aagccaagct
    27661 gcagttcctt tggtcaccac aactgggatt ttacgatcac cacttgatgt ctgaataatt
    27721 tttgcaagtt tacgaaatac attctcttcc tcaagagttt ggaccagggt acgctcaaat
    27781 tcatcaggaa caagatatcc tccctcagaa tcagtaccaa cagataaagc atttagtgca
    27841 tcatgacgag ggtttttact acgcattaca ttccaaaatg ccttcctgta ctcatcacta
    27901 gctcttccgg tctttgtatc catccccgga atagctggct tttcggtaag aggtgtgttt
    27961 acaggcttat taagctctgc ttcaagagct tcttggcgct ccaatcttgc gatttcctta
    28021 cccagattaa taatatctgc ttccattttg tcgtaggttg cggcatcctc tgcggacaca
    28081 agtccatcac taccacgctt tgaatcaaga aatgctttag ctgcttccca tgcttttgcg
    28141 cgtttttcac gcagttcaag aattttactc attttcattc ctcctaatat ttcaataaat
    28201 taagccgctc ataaagcggc tctactgact gttttacaac tggtttttga agtttattca
    28261 ttagtgaatt ggtcactgct cttctgctga atacaaagct gtcttgtagt gaactctctc
    28321 ctggtttaaa cataatgtca tctgcaaaac ctagctcaac tgccttattg gcatttagcc
    28381 aagtttcagc atccatcagg tgcgacaacc ttgttctaga taagccggtt ttaagctcat
    28441 atgcattgat gatgctctct ttcacttcat caagcatttg gattgctttt tgcatctcct
    28501 cactatcgcc aatggctatg gtgaaaggat tatggatcat catcagtgag gtaggagaca
    28561 ttaagacttc cgtccctgcc atagcgatga cagaagcagc tgatgcagca ataccgtcaa
    28621 tctttacagt gacgtttcct ttataatcca ttagcatgtt gtatatctga gatgctgcta
    28681 tgcaatctcc accaggagaa ttgatccaaa ctactatgtc accttcacca ctcattagtt
    28741 cttctttaaa agcagctgga gtgacatcat cttcaaacca actctcctct gcaatagcgc
    28801 catttagata tagggttcgt gtctgtgtat ctgcatcacg aacccaattc caaaatttct
    28861 tcattcaggt tttccctcca ttccttcttt atttgcgaat atgcctgcgt ccgcaagctt
    28921 ggtcatattg ccattgatta gatataagtc cccgccaagc tcaggtggga ttcgatcaag
    28981 gttctcaagc tccctaatat cattggcact catccaacca ttctgtctcg cggttgcata
    29041 accgttcatc cgagagacat aatcacctcg aagcagtcca tccacattaa acttggcaaa
    29101 atagagcttc ttttcatctg gtcttaaaag tgatcggctg atggcctgct cccaacggat
    29161 cacccaaggg tccaaagtgt atttcacaaa ctccagtgac tgttgctcaa tattagaaaa
    29221 gctcgatttt tccaagtccc caaccatatg tggaggtacg cggaaaatcc gagcgatttc
    29281 attaatctga aactttcttg tctctaaaaa ctgagcttgt tctggtgaga taccaataga
    29341 ctgatacttc atcccttcct caagcactgc cacacgatgg gagttgctgc ttccttgata
    29401 agccgcattc cagctatctc gcactttttg tgggtcttta atcgttccag gatgctcaag
    29461 tacacctccc ggagctgctc catttgcgaa aaacttagct ccatattcct cacaggctat
    29521 ggccatacca atggcattct tggccattgc aattggagaa taccctacta gtccatcaaa
    29581 tcctaagcca ggaatatgga gtacatcgct tggccttagt gtgactgtca ttccattcat
    29641 cgtaggtgct tcatcggaat atctggtgta ggaataatat aaagaaccgc tggaatccct
    29701 atccacagac attcgattag gcattaatgg gtatagtgct atgacttctc ctttgccatt
    29761 tcgaattatt tgtgcatagg cattacccca taataaaaga tgagtcatta acgtttctcg
    29821 gaaaacgaaa gaactcatct ctggatttgg ttcatcatgt aataaaaaat ataagggatg
    29881 tgatattgct ttctccttac ctccactttc tgtgtattta tataagtgaa gcggaagtcc
    29941 tgccacagcc tctgccagta ttcttacaca agaataaacc gctgtcattt gcatagctgt
    30001 atgctcattt actggtttac cactagtagt tcctccaaaa aagaagctat aattactacc
    30061 tacggtccgg ttctgaggtt tatcacgggc tttgaaaata tttgataaaa gtcccatctg
    30121 catcactctc ctttgctaca aaataagaag ccctcgaccg tcataaacag aatttcctga
    30181 tccacctttg cggattgctc ggtcaagagc cataatggta gccacagctc catcaattct
    30241 ttcagttgat ttttctttat ctggtttaat atttccagca ggatcagtac ggataaaaat
    30301 attatccatc atccatcgca gtacaggatg tccaccatgt gctagttttt cctctaatgt
    30361 taacttcatt agctctttag ttggagggct catatcttta aagccttgac caaaaggtac
    30421 taccgtaaat ccaagatttt caaggttttg tgtcatttga actgctcccc aacggtcaaa
    30481 ggcaatttct cgaatgttat attttaagcc cagctcttct ataaagtttt caatgtagcc
    30541 ataatgcacc acatttcctt ccgtggtgta taaaaagcct tgctgctccc agatatcata
    30601 tggcacatgg tctcgtctca ctctctgttc aatgttatct tcaggtatcc aaaagtaagg
    30661 aagaataata tatttgtcat catcatactc aggtggaaag acaagaacaa atgctgtgat
    30721 atcagtggta gacgataaat ccaaaccacc atagcacaca cgtccttcaa gactttcctt
    30781 gtttatagca aaagcacatt tatcccactt ttccattggc atccagcgta tcgactgctt
    30841 tacccattga ttcaaacgca actgcctaaa caggttctct tctgctggat tttgctttgc
    30901 actttcacaa gcaatttgaa tcttttctat atcaacggta atgcccattg aggggttggc
    30961 tttcgcccac acttttggat cagtccagtc atcatcttca tcagcaccgt aaactactgg
    31021 ataaaatgta gggtcaacct ttcgcccttc caagatgtct ttagcttttt gatgcacctc
    31081 gtaacaaatt gaatgtgtgt cattgccggc agtggtaatt aggaaatata acggctgctt
    31141 tcttgcatca cctgaaccat gggtcataac atcgaaaagc tgtctattgg gctgagcatg
    31201 tagttcgtca aataccacac catgcacatt tagtccatgt ttggtatagg cttctgcaga
    31261 taaaacctga tagaaactac ctagcggttt ataaacaagt cgtttttgcg atagtaccgg
    31321 tttaattctt gctttcagag ctggacattg ttctaccata tccactgcta catcaaaaac
    31381 aatggaagct tgctgtctgt cagatgcaca tccgtatact tcaccgccat gctcaaaatc
    31441 accacaggta agcagtaacg caaccgctgc tgcaagttca gatttccctt gctttttagc
    31501 gatttcgata tatgccgtat taaattgacg gtaaccatta ggctttataa tcccaaacac
    31561 atcgcggata atctgttcct gccagtctat aagttcaaat ggttgcccat accattctcc
    31621 cttcgtatgt tttaagcaat taataaaaga caccgcaatg tctgcagcgt ccttatcata
    31681 taccgaacca tctgccttaa agatggtcgg cttatatttt tttagtttac gtatggccgc
    31741 cacctcctta tggctacgaa aaaaggaacc ccgaagagtt ccctaagatt taatagtcta
    31801 tttattattc tgtttctact ttccctgtca gaataaaatg gacatacgct tccttattct
    31861 tttcaagata atctataagc tctatatatc cttctcttaa agcaatgctg gtcaataagg
    31921 gaagatcaaa catattagtt tcgcctgtat ctctaatggc aaaaatctgg ctttttataa
    31981 tgtcattcat tcttttcctc ctcgattttt ctgcaggaat cctcgccata tgcaactcct
    32041 aagctgctac cagagtccca attgacatga atggttccaa tatcatctac accaatgaca
    32101 gtacctcttg ccccaggttc tagtttggta taggggtcat tcatctttac taaaaccact
    32161 cttgttccag gtgtatagac actccttagt tgctttatta tttttgggtg aacggttttc
    32221 aatttacatt cacctcctca cgcttaggaa ctccactctt gaatgcagaa ctgccgctta
    32281 gtttggaaag aagtattctt cttgatgtct tgtattcttc tccaataaaa ccaagtctta
    32341 ataagaagca tcgaaaggca tatttttcgt tgtctgcttc tttagctgtg gcagtgattc
    32401 tcttttgctt ctttgccata tcacaaaggg ctgtaataaa atgtgtatat gctttaacct
    32461 tctcagcatc tccgtcaagg taaaaccaag gaaaccggag cgactcctct gtttgttcaa
    32521 caggtagttc ctctactcca agggccttct taatgagagc tcctttgctt tccagaatct
    32581 tttccaagtt gctataggct ttatcattga aatctctctt aggaatttca ataactagca
    32641 agtctttatc ttctaaatgc tctgctagca aacaatcctg tttcacatat gaaaatcctt
    32701 gcctttcaag ctcatctagg agtatggtcg ctttttcgcc aacaacaccc tcatcaaaaa
    32761 taagagcacc ttccttattc acttcaaatc tgcctatttg ataaacaaat gttggtgcgc
    32821 ctttgtattt aggcttttca ccaagtattt ccccaatcgc ggtaactagg gccttccttt
    32881 cactgccagt gcgattaaat tcaattttca tgctacatac cacctttctt atttggtagt
    32941 aacatatatc actctaaagt tacacaatag caagtcattt ctttgaatga atgtataact
    33001 tcttctatca acatatttcg tcagaagatg tagctacccc actgaatgga atcttctctt
    33061 tttgacgaat cacatagaca tctttatctg ttcctacctg ctcaatgtaa cgcttcacaa
    33121 taacatcaca gtatttttca tccagctcga ttgtatagca aattctatca gtctgctcac
    33181 aagcaattag ggttgagcca gaacctccaa aagggtctaa cactatacta tttgttaggc
    33241 ttgagttcat aattggataa gcaattaggg aaacaggctt catcgttggg tggtctgcat
    33301 tctttttagg tttatcaaat tcccagatag ttgattgctt gcggtctgaa taccagagat
    33361 gcttaccctt ctttttccaa ccaaagagta ctggttcatg ctgccactga taaggagatc
    33421 ttccaagaac caaggattgc ttcttccaaa tacaagtacc agaaagataa aaacctgcat
    33481 cgacaaatgc ctttctaaaa ttaagtccct ctgtatcggc atggaataca taaatgcttc
    33541 catctgttgc cagtgctgtt tcaatattta taaaggcatc taatagaaac ttataaaaag
    33601 catcatttgc catattatcg ttcttgattt tccctgcagt accttcatag ttaacattgt
    33661 atgggggatc agttaccacc agattagcaa cttttccatc cattaatgta ttgaaggtct
    33721 ctttatttgt actatctcca cataccattc tgtgcctacc aagtatccaa acatccccta
    33781 aacgcgaaat agcgggctta ttcagctcgc tatctatatc aaaatcgtct tcttttattt
    33841 tgtcttttaa ataatccttg aaaagatcat ccagttctcc tggatcaaat ccagtcagag
    33901 atacatcaaa gtctgcagcg tttaaatctg taataagaag tgccagtttt tccttatccc
    33961 attctccgct tattttattc aatgcaatat taagagcctt ctccttttcc tcatccatct
    34021 ctacaactac acaatccact acatccatac ccatgctcag tagaactttc agtctctgat
    34081 ggccaccaac cactctacct gtagttttat tccaaataat tggttcaaca tacccaaact
    34141 cctcaaatga cctttttagt ttttcatact ctggatcacc tggccttaaa tcctttctag
    34201 gattataatc tgcaggaatc aacttcttga tagaaatctt atctatattc atgctaacct
    34261 cccaaaataa aaacacgcct catttaatag aagcgtgtta ttgattgatg aatctttaag
    34321 ttttgctttt actatttgaa tatatcccag ttgttataat aggcagtcat atctttttcc
    34381 gtctctctga cttcaagtgg tttaccatcc tttgttactc ggaaatagaa gtggagtttt
    34441 ttgttggcat cataagcctt atcgtcaaac tcattaataa ggtcctcacg tttgaaaata
    34501 aagtaatgga attcactctc tttgccttca actacaaaga tgaaatatgg gaaatctgga
    34561 ttatctattt ctttatcaaa tacagtatgc catccagaac aaattacctc atcgctatca
    34621 gtgtatgaac gactatatga aaccttgata ttgtatgatt tcccattctt acttgctaca
    34681 ataccacttc cttcagagcg atttcctttt cgaacactat acccatactt ttcttctaga
    34741 attgtcataa tctctctgcg ggttcgtttc cttccacttt cctttttttc ttctccgcta
    34801 gttatatcat cttcaccctc atctttgttt tgcaactctt gctggtattc ttgtaacata
    34861 tctacttttt cttccaggtc ctttatccgt gtaagcattt caagaattac tttttcataa
    34921 ttcatcgtaa cacctccttt aatttatcta atttaatcat atgttatttt tgattttttg
    34981 tcaatgataa atttagataa attaatgata aattatggtt atttagataa agttgattct
    35041 gtgctttcta gataaaaagt ctggagcatt tttttctagc agataactac ttatacatat
    35101 tccctatcta catatttttc caccgctgct tttaattcat tgtatctatc aacatcctcc
    35161 caagggaaaa gacaactatt aaaatgtcca taaaccgctg tatcagagta gtgaatgttt
    35221 ctaagtctaa gcttttcaat aattgctgca ggccttaggt taaaaacctc ttgagcagca
    35281 agggtcaaaa cttcatcaga aactgttcct gttccaaaag tatttataga aaaagctaca
    35341 ggatttgcct tgccaatggc ataggaaata gccacttcac atttctctgc caaatcacac
    35401 cacacaatat gtttagcaat atacctagcc atgtaagctc cgcttcggtc aactttggtc
    35461 ggatctttcc cacaaagtgc gccgccacca tgtgatgcaa gtccaccata agtgtccacc
    35521 atgatctttc ttccagttaa acctgtatct gcagcaggtc ctcctaatac aaactgtcca
    35581 gatggattaa taagaatttc tgtttcatca tcaaaaggga aatcctcaaa acactgccat
    35641 aacacattat ttagaatatc tgattttaac tcttcctgag ttttattctt ttcatgctga
    35701 actgacacta caatggtctt aactctcact ggaacatcat catgatactc aacggtcacc
    35761 tgtgcttttc catcgggaag gataccttta ataattttac ctttccttgc ctcatctagt
    35821 ctctttacaa ttcgatgaga taagacaagg ggaaggggaa gcatttctct ggtttctttt
    35881 gtggcataac catacatagt accttgatca ccagcgccga tggaaccata ttgttcgttt
    35941 ataccatttc gtgcttctaa tgcagtattc acaccagctg caatatcaga actctgattg
    36001 tgtacatata cataaattaa aaatttcaaa gggttataac ctagttcttt tagcacattc
    36061 ttaacaatgc ttctgatatc aattttctcg ctgcaggaga tctcacccgc cacgataatt
    36121 ttccctttag tagccataac ctcacacgct acgcgtgacg ctttatctct tctaaggcat
    36181 tcatccaaaa tattatcagc aataatatcg cacagtttat caggatgtcc tgcacataca
    36241 ctttctgctg ttaaatatct cttactcatc tcacatctcc ttattattta cctctacggg
    36301 cagtcaacag tcgttccatc acatcatcct gtggatttgt accggaatac tctgtcgcac
    36361 agttttcacg aacaatctga tatatttcca tccagagtct gttggtttga ctcataaaat
    36421 tatggctcat ggaaacgtat ggactttgga tagcgttgcc agtagttgga tgcttagcaa
    36481 gaaaaccaaa ctcacttatt gcttcctcac actgtatcca cctagcagca ctcatggcat
    36541 atctttctaa tagctgtggt agaactagat gtgcacaccc tcgctcctca agccatttcc
    36601 acgtaagctc ataaatttcg ctggctacta aggttttacc atccttttgt actgctgaaa
    36661 gcatagccct tggctgtggc atctcctgcc cttgaagttc tgcggtattt ttaaattcaa
    36721 taacttccaa ttttcttttt ccgggatttc cctcagcaat tttatctata agtgctttct
    36781 ttttctgacc agatccaata cgggcaccac ctcgatttgt accatctttg gccattaact
    36841 cacctcattt cttattaagg gggtattacc ccgtttgaaa ctgcgacttt ttgcacgaag
    36901 ccccacgccc gttgaccgtt taaaagctgt agagatttga ctccccctac cgggatggcc
    36961 aacggtctcc atctcttgct gtgatagctg agtgacaagg agtacaaaga gccattaggt
    37021 tgctttcatc gtgtgtccca cctcgtgcca agggaaggat atggtgcact tcagttgctg
    37081 gtgtcagctt tccttgtctt ttacactctt cacatagtgg atgggctgta atgtaacggt
    37141 cacgtattct tttccatgca cgaccgtaac gcttcctcgt ttcaggatct ctctgatatt
    37201 tttcataacg agaagcttct ttttttgtat gctccggaca aaagcgtcca tctgtcagtt
    37261 ccggacaacc aggataagaa catggtcttt ttggtttctt tggcatttcg cacctccttt
    37321 ggacatacaa aaagccctcg tggtatttcc acgaaggctc tctacaattt ttcacaatac
    37381 cattgtatta tggatttcta ataaaatcgt ccaagatatt actcattact ttccatagag
    37441 tagtagtgct aaatgttgaa gcgctcgatt ctttttgttg tatgctgaag aacgttctat
    37501 attgaaatga tcacagatgt tatacacagc atcaatctgc ttttgttcat catccaaata
    37561 aaactccttt aacacatact gctcgtcttc tgttaaagca tcccatgcgg gttgaaacca
    37621 atccatgtat tccagtgctt gacgataacg ttctttcaat acatcaattt cattgatgca
    37681 ggcaatgagc cttttctctc ctgcttttgg atcatgggtc gatggcatgc cattgataac
    37741 tggagaagct ggggagctca tttcttcgtt gagggttgca atgtcctcat cggtatgttc
    37801 tatgatgtac ttcatgctgc tgtaatcttt taaggcatta attgccgctg ctcttttatc
    37861 taaatactgc cagacaatgt tcatcgtatc agacctcctt tagtgttgct tttaccgcat
    37921 ctatcagtgc ggattgagta ttgtttttat catttagggc tttcattaca cgctcatcaa
    37981 tggtgccttt ggcaatcagg tgatggatca ctaccgtttc attttgtcct tgacgccaaa
    38041 gacgggcatt ggtttgctgg taaagctcca agctccaagt caggccaaac catacaagag
    38101 ttgaacctcc agcttgcaag ttcagtccat ggcctgctga tgctggatgg atgactgcaa
    38161 tggggatttc tccatcattc caccttttaa tagaatcgct agtggataat acctcaactt
    38221 caaagcgctt ttgtattcgt gataaatcat gtttaaacca ataagcaatt agaactggct
    38281 tgccattagc agcttcgatt aagtcttcca gtgcatccag cttacggtca tgtatatgaa
    38341 taactacacc ttgatcatca tagactgctc cgtttgccat ctgcaggagc tttcctgaaa
    38401 gagctgctgc attggctgca gtaatttcct cacctttaac agttgtaatt aaatcacgct
    38461 tcataatgtc aaggatttcc atttcttttt cagaaagctt tacatctact tcgtttataa
    38521 ccaactccgg caacttcagg tagtctgtcc ctttcatgct aatagtgatg tcagatatga
    38581 gcctataaat agcttcttct gctcccggtt ttggtttgta ggagaagatc acttgctgat
    38641 tgcgtttatc tggtacgaaa aagtcctctc gatatctgcc aataaacctg cccaatcgct
    38701 gtcccatatc cagcaggcga tactcagccc ataaatccat cagtccattg gatgatggcg
    38761 ttccggtaag ccctacgatc cttttaatct ttggcctaac tttcattaaa cttttaaatc
    38821 tcttggcttg atgggatttg aaggatgaca gctcatcgat tactaccatg tcaaaatcaa
    38881 agggtatggc acttctagaa attagccatt cgacattttc tcgattgatg atgtatacct
    38941 gagctctttt cataagggct gttttcctct gtacctcaga gccaactgat acggtgtatt
    39001 taagaccttt taggtgatcc cacttttcaa gctctgcagg ccatgtatct ctggctactc
    39061 gaagaggtgc aatgaccaga accttacgaa caagaaaact atccagtgtt aaatcaaaga
    39121 tggcagttaa ggtaatgaca ctcttaccta atcccatatc taaaagcact gctgctatgg
    39181 gatgggtgag gatatactca gtggcataaa cctgatattc atgaggcttg tatttcatga
    39241 agtatccctc caatctgttc tatgtgatcc aggcagaata ccaaaaatcc aagtgattct
    39301 aactgtcttt ttcgcttttc ctgtagaggc cttaaggttt tacctggagc ttttacctcc
    39361 acaaaagcta tttttttatc cggcaaaaga atcaatctgt ctggcattcc atcaaaacct
    39421 ggtgatgcaa tcttcagtgc aatgccgcct atatctttta ctgcttttac cagttgttgt
    39481 tcaatccatt tttctctcat atagcctcca tgttccctaa atccaaaaag tctctatacg
    39541 cgcgtatata cgcgtctgca ggtaatttct tctttttgtc tttaggatta ttattaataa
    39601 taatttttgg aacaatggaa cacagactat taagtagcct actttacaag gggctgccgc
    39661 ctgttccgat gagatgtacc aaaagaccgt ttttgttcca ccggaacagg tgaaatctgt
    39721 tccaaagaaa taattgttcc atgtgttcca aactgaatta ttcttttgga acataaaccc
    39781 actgtggtcc ataaagctgg atacgttctt tttttacgag acctgtccag cctccaatac
    39841 tcgccataat agctgatatt tcattaccat ctactcttcg aaggttggct cgatcctttc
    39901 caaagcactc acaccagatt tccatattgg ataccgattt acgtttccaa acaccaaccc
    39961 tctggctttc accaaactca gtcccgttga tataggctcg gcgttcatat aaatccatat
    40021 tgtcccaatc ctcaggcaaa agcatatcaa ggtactctcg taccaaacct tcacgctcat
    40081 ccgattccat agcttcccgc tgttcttctt ttgcaagctt ctcaagactg gcatcaaggt
    40141 acaatttctc tccagctttc acataggtaa gagcctcagc ccatatctgc agaatttcat
    40201 cctgctttag ctgccaagac ttttttgtac catttcccgg agtctttacc ggccagaagc
    40261 gacggtttcc tgtggtatcc ctcaaatagc ctttctcagc attggtggta ccaaaaaaga
    40321 tacattgtct taagtgggga gtagctcttc tgccaaaact agctctataa atgtcatttt
    40381 ggcgagacaa gaaacttcga agcgtttcga cttcagcttt tttaagtccg gccaattcac
    40441 caatttctaa aatccagtaa ccctgaagct tttccgccgc agtcttgtcc ttggtatccg
    40501 ataagttcaa gctatctgaa aaccagtctc cacctaactt ggcaatgaga gtgctttttc
    40561 caactccctg tggcccattt aaaaccaaca tggaatcaaa cttgatgcct ggagtcagta
    40621 cacgagagat agccgcacat agagtttttc ttgtgacagc ccgaacatat gggttatctg
    40681 atgcacctag ataatcgatg agcagggtat ctactcgcgg taccttatcc cactcaggaa
    40741 gagcctcaat aaattcccga atcggatgat aagatctatc gtcagcgacc ttcgctaccg
    40801 ctacatcata gtttcttgca gaaaaggttc cgtagtgggt gtcaatgtag ctgattagct
    40861 gtgcatcatc tgcatctctc cagaacttcg atggatgcgg ccaaggaaca tcacctttaa
    40921 tttcgagact atcagatagc tgattaaaca ctatgctttt taggttggga tcattttcaa
    40981 gtatcaaaat caaatttcta agagtatttt taaccgttcc tgtcttatca atctccagct
    41041 gcttttccca atcttcatca ataaactctt tttcagcctg agcctttcgt tcttcagcaa
    41101 actgctcttt tacccgttca tcctctaatg ccaaatctgt catggctcta aatgaaggta
    41161 gtttacttgg cggtgtattt tctgtcacct tttcatctag ttcccgaaac ttatgcactc
    41221 ggaccaaatc aaatgcgttt agcaacattc cacatgctgg atcggtagca tgatggctat
    41281 aagcaaattt accatcatag attaccaagc ctgctgaaga atctgctgga atataatcaa
    41341 atcgtccatt catggtacta ggttcatata catcaggtag aaattcttcg atggcttctt
    41401 caatgttata ggctctgcaa aatgccccta taactccttc tttacttaag ggatctgctt
    41461 gtttagttat ttttctttgc actacctcag attgccgact tgaaactggc cacattgaag
    41521 tatcccgcca gtcttcatat tttgaaagat agccatctgg gtctaacagt tctccatctt
    41581 tttctttaaa gacaaactct ccatctgacg gtgtagaggg ccaatacatt aatctcgaag
    41641 gttcataagt ggtgtcatcg aataaatcaa tccctatctc ctttgcaacc atacgaccta
    41701 gagctgggta ttcatcctct gtcacttctc ttttaagtgg aattataagt cttaatcgtg
    41761 gtgcatcagg tgtgtgtttg tgagttgagt agatgcaaca tttaaaatca tgcaatgcat
    41821 caatttgatc ccaaatccct ggttttccat aatccatatc taatgtaagt agggagcggg
    41881 agagtacata gccatttcta cgctttcctt cacgtagagc tcctcctacg aatccaccca
    41941 catcttttat tgagtcctgc tgtgcacgac tcattttccg aaattcagat accgtttctg
    42001 tggttcgtat tgtagattta actcgggata tgaagtcctc ccatgttatg tctttgttct
    42061 tccacttttt gtccattcgg ctgttaccaa ccgatatctt catagcttct gcacctcctc
    42121 gcagttttca gtaaaatacc gaatagccat acgatgcttc gatgctttat ctatttctgc
    42181 ttgcattcct tttgaaatat agggaccaaa cacccacagc tcctcacact ttcttagcca
    42241 gaccatacca aagaataatc ctagttttct ttgttcagga tcactatcat ctagcacttg
    42301 aggatatagc agatgaggag caaaagggat ggttccttgc tccactgcaa actttaagta
    42361 tcctctagcc tttttcagat tttcttctat atccccagca aaaggtgagc agataaagac
    42421 acaaggttta tagttttttg ctttttcttc acgcatgata ttttccaagg cttctgctgc
    42481 agttggatct ggatagcctt cagcgttata tctatccata agttacatct cctcgaattc
    42541 ggactcttgt tcaatcaatg gtaaaatgcc atgatcctta agaagctcat aaatgaacaa
    42601 gcgtcctttc tgagtccagt aagtatgaac ctttgaatgc atcgtcccat catttccaag
    42661 gtaagtatgg gtttttgtcg tcgtatatcc atgttgagcg tacttttgat atagaagcca
    42721 aatcttccct tgacggaatt gaacacctaa attatgcaga tactcattaa accagcgccc
    42781 tgactttcca tagtccttgg ctattgttgt gatagacacc gcatccttac agttaagtac
    42841 cacatcgtaa taacttgctt taggtttcat ttctgcaatt tgctgttctt gaatgctaat
    42901 ggtagtagtt aactcagcat ttttagctct ttccgcttta agctcctgta gtgcctgaat
    42961 caaaaagtct ggattagcaa gtaactcatc tgtagcataa aggccatgcc ttctgatgga
    43021 aggaagcacc tcatgggtta cccagcgctt aaacttttta gcttctggct ttcgagaaac
    43081 caatatcacg ctataaagac cactttcatt gataatagat acttcctgtt ttcctccagg
    43141 ggtgtcgata atatcgactc ccttttcatc actgtccaag cgagccataa catcacgact
    43201 gtttccaatc tctaatactg agcacacatc tcttagaacc caccaaggac ttccatcctt
    43261 cattactgtt cttactgtgt taccttcgta gttgaaaata gttaatttgt tcatatcgaa
    43321 cctccaacac atatagtagt agagcaataa aatctgccct cagctatatg caaaaaaaga
    43381 ggaggcttcg aaccccctaa aattaatctt ttttataaaa attacaatca aacccatcgg
    43441 cacgaagcaa aagtccctga gcccatggag gtgtatgtcc cataagagca caaatatctt
    43501 gaacagatat ttctattgga acttctaaaa ctacctcatc gtggacatgc attacaatat
    43561 caaaatcttt ctcatcaagc tttctcatgg aatagcaaag gatatctctt gaaagagcct
    43621 ggacgatatt ctctacaaac ttaggaccat agctttcaat acgctcccac ttctttgtgc
    43681 caccaatgcc ttcataagtt accgattcac tgccaaagct gttaatacct attcttggtt
    43741 taacataagt aagttgtctg ccagaaggaa gccatattaa tagcatcccg ctacgatact
    43801 caaaacgggt gcgatgagtt tcggttctgc acctttctgt tacagcttct ttgacagcac
    43861 gatcaacatc ccaccaaagt cgtacaatat taggatttgc atttcgccat gcatagacca
    43921 aaggttttaa ctcttcttcg gtaagaccca tgtcaagtgc acccattgct tttaatgcac
    43981 ctacagaacc accataacct aaggccagct ccgcaatttt tcctttttgt ctgagaggac
    44041 taccttttgt gacttcttct aaggggactt taaacatctg ggaagcagat gcttcataaa
    44101 tcttgccatg agtagcaaac acttcatttc tccatgtctc gcctgcaatc caagcaataa
    44161 ctctagcctc tattgcacta aagtcagcaa cgataaactt atatcctttc tttggaacaa
    44221 aagcagttct gattaattct gataaaaccc ctggaatgga gtcatataat aattccaagg
    44281 cttcataatg accacctcta accaagcaac gtgcctgttt cagatccaac aaatggttct
    44341 gaggtagatt ttgaacttga attaaccttc cagcgaatct accggttcgg ttggctccat
    44401 agaattgaag tagtcctcga gcccttccat cagcacatac tgcattttcc atagcagagt
    44461 attttttaac actggactta gctaatagtt gtcttaattc cagcacttca ctcagatgat
    44521 ctggagcatc ctttaataac tccttaactg atgccttatc aagactatct gtttctaagc
    44581 cattttctga tagccatttt ttcatctgtg caactgaatt agggttttca agatcagtta
    44641 ggtcttttag tctgcttgtt agctcttctc ttactttttc atcacattga atcgcttttt
    44701 ttaccaattc caaatccaac tgaatacctc gatcattgat ttgctgatct agaatatagt
    44761 tattccattc ctcttcaggc ataggaaatt tctgaagctt ttcctgtata gaaagttcaa
    44821 cttcaacgtc tcgaaggtta tatgccttaa atctatccca tttatctgga gcatgttcag
    44881 gtaagtttcg tgtacgacca tcatttgacc ttgtaggttt gcatggtaca gagaagtatc
    44941 ggataagctc ctttccttct gtcagctttt tcttatcaac tcctgttatt attgctgctc
    45001 cttccagtga taaaggaagt ccaagatagg cagaccacac catggtgcag cgccaagaat
    45061 caggttcaag ccaaacacca aagtatttag ataagcaaac tctttcaaac tgtgcattaa
    45121 atgcccattt tataacctta ctatctagaa ttgcactttg tatttcttgc gggatagcct
    45181 caccagaagc caagtcgacg acctgaaccg gcccagcatc cacactgtat ccaaaaagta
    45241 taatctcaaa atccggtgct tcagcataac gatagacccc gcttttggca aggtctacac
    45301 tactatatgt ttctatatca atactgagag ttctcatgat aaaaagtcat cgtccacgtc
    45361 agtggcaaag tcatcagctg cattagttct tccacccaaa ggctcaccat cgcggatttt
    45421 ttgaatattt ccaagaccac atgctatacc cttattccca ttggagttaa aggcataaaa
    45481 gttgatactt actcttgcat aaacaccgga gtatacttct gaacgctcaa ggattggatt
    45541 gacatttcta tctacaattt gtggagctgt aatactgttg gcattgataa aatagctatt
    45601 tgcataagcc tcatcatctg gacgatcaat atctccgtca cgcagaggaa gctttaacgc
    45661 cgctttatta ggaatcttgc caccaaactt acctctacct tcttctattg cagcattcac
    45721 tgcttcgtta atagcactta aagttttagt atcactcttt ggaataatca ggcttacgct
    45781 gtatttctca gcacctccat tgatggattt tggttcccac acattggcgt aagaaagcct
    45841 aacaactcct gttactactt tcgttggatt tgttcttttt gctgttgttg acatagtttt
    45901 tatacctcca taaaatcatt ttttgctgat gatgtgttca tttcaggacg cttatcagaa
    45961 attggtacta gtgttggttt tcctggtggc ttcataacta gcccgccaag gacttcattg
    46021 aattttgact tacccattaa tttttccatt tcggtaatgg taataaggct ttgcctatat
    46081 atgtcttggt aacctgcatt ctttgccgcc tgtgcgactg cttcttcatc tttgtattta
    46141 cggttggagc ggccttctac tactttaaag cctggccact tcttgccatg attaactgca
    46201 gcatctgttg cataggctat aatctcatta gcccaacttg taaggtcgct gatggaagac
    46261 agaatatcag taatctcttc atcagttaaa agtggcggta gagcaaactc gaatcttgca
    46321 agtttcattt ttgcttctgc tcttgcccta catttaactg ctgctctaca aaactgacac
    46381 cattctccag gacagtagat accgtcacca gcaaaggcta attctgcttt aggcttcaat
    46441 acttcttcag cccattgata taagctctct tttgagactg tagatgtact aacattttca
    46501 cgacgtggtt gatagattgt cattgaaacc atctcgatat cataaatgcc atcaaagaga
    46561 tctagtgcac caagagcata tagtttcatt tgaggattgt cctctgcact gactaagact
    46621 ccctgaccat acttaaaatc aattatgtta agaaccttat cagcgattat tatgcaatcc
    46681 ccagtcccaa atccatcagg aacatacttt gaaaaatcaa gtcgctgttc tatcaagatc
    46741 aggggatcac tacaggcctg cttggcttgc tcaatcactt caagcacaaa ctccacgtaa
    46801 ccatcagtat aattgtccat ctcatctgag tcatatggag aaaccggctt ttttgatctc
    46861 atcttaagcg ctttgcggag cttgtgctca cttaacgcat gggcagctgt gccttcagcc
    46921 gcagcttcac cgctgttgtc gtcaaactcc agctccaacc tcgctgatgg tgtgcagttt
    46981 aaccacctat gggctccaga tgcagagaga attgcatgtt tactcatttc aatccctccg
    47041 catcggcaag aagtgcagca tacttgcttg gatcaatctg gctgagtttt gatgcaccat
    47101 acttttctag aagtgctctt acttcagctg taaatccgtc atggcttttt tccgcaagta
    47161 ccgcccttac ttcttctaag gtgagctgct tttcttcaga ttgtttttca ggctttggtt
    47221 gctctggctt tgtaatctca tgaggccctt tacttgctat tgcatcagct acagcctgaa
    47281 cactatctgc cagagctcta agatctgaca ccacatcgag aaggagctta atcttactca
    47341 tgacccacac ctccttcctt gatttccttt atttctaccg tctcaactga gtcaccggga
    47401 gtaattacaa gcagactgac tttctttcca aataggaaat caagcattct cgctcggatt
    47461 gtcttccgac tgctttgaat tactgagctt ctttcgccac cgggccttgc cacattgatt
    47521 gtgactttgt gtttaaggtt catattccat ctcctttctg ggggcgattt atctgcccct
    47581 caccgataag cgaaaaagag agttctttcg aacccccttt tcagaaaaga tttttttata
    47641 aagggttctt gcgaacccaa ttaggataga atttttctaa gtctttcttg aagcttcttg
    47701 aggcgaccac gaatagccgc ttctgaaaca ccttcttctg ttgcaatatc agtgttggat
    47761 cgtttttcaa ggtatacttt tttgaagagt tctttttgtt gtggtaaaag acactccatt
    47821 gccttggtta acttgtccag catatcttga tgttcagcct catcttctgc ttgtataatt
    47881 agctgttctg gattagcagt atcatctgca agatacttat tgcggtcatg ggctgcttcc
    47941 tcttcgccat cgtgataggc atccagatgt gctgttattc ggtaattata ccggcgttgc
    48001 tcgtccactt cgacgtcatc catctcatgt aaaagctgaa tgtaagcttc ggtgacgcca
    48061 tcctcacctg gggtaattac gatctttgct ccttctgcag tgtaataaat atagttagtt
    48121 ctcttatttt gacttgtttt gtacgctctt gacatgtttt tgcatccttt ccgccggatt
    48181 gcattggcgg caaaggatac aaaaataggc ctgtaccaga agtacacaga cctttttatc
    48241 ctgaaaatga gcgcaataag gtaaggtact tctattgcac cgcaacaatc cttacggatt
    48301 ggagcgaaac aatatgtatc ctctgccctt attgcaaatc aggcattcga tatttttttt
    48361 attagttggt aagtggtcaa gtttttctat ctgctaaagt aagcttgtct taccatctat
    48421 ttaaatttta ataatttggg cgtctgaaat cctcagctgg cactctgctg atactccgct
    48481 ggtactccgc aaactctttc acaataaaaa aactaagcat ttgtaaagaa atacttagta
    48541 acattagatt attgattgaa atacgcttag taaattgcta taatggtaaa tatatatata
    48601 tcaataaaac catccaatag tgtttggagg gcgttaaaaa tggcatttag ttataataaa
    48661 ctttggaaac ttttaattga taaaaagatg ttgaaaaaag atctgatgga aaaaacaaat
    48721 ttaacttcaa caacgttggc aaaaatgggt aaagatttgc cggtaagcat ggatgtatta
    48781 ggaagaatct gtaaagctct tgatgttaac attggcgata ttgttgacta cgtcgatgat
    48841 gatactccga agaaataaaa ttatttaagg gggcgcaatg tatgaaaaag ttatgctttg
    48901 gtacttatgc aacaatttta aagagatgca tggctaaaag agttacacaa aagcgattgt
    48961 gtggtaccat gctgctttct gttgcgccta cttatgatat tagtggtgaa gatggcacag
    49021 tttctgattt aatacttggc aaaaaaaatc tatcccccat agtaactgat acagctcccg
    49081 gtgccgatcc tcatgatatc tcagattact ttaaacaaag tattcttcag atgttggata
    49141 gcaataagag gagtttaatt gttttggctc tgaaagacat tattacatcc gataactcca
    49201 tcgagcctga tacaattgtt gaaatagtaa atggcatgac aaagcaagat atcatgaatc
    49261 gtaatgcctt tgtattggag gaattcctcg caggagtctt tctatataca gtgctttacg
    49321 tcgaaaatcg aaattgtgag agtagtgtta gtgaaataac agatgaatac atacaggcct
    49381 ttgaagatca aaaattagat ataagcttta ttaagacata tagcaatttg tctcaggata
    49441 ctgcctacga aattgcaatt gatgcccact ctctcgtatt gcttgcagaa acgggtggca
    49501 aatgccaaaa gtgcggaaga gtcttaggta taaaaaaaga aggcaacgac attaactacg
    49561 ctaaagtcgt tcgcctttca gaaactgacg aagtggttct atgtgttgaa tgtgagcgag
    49621 aaattcagag tgcctccgaa gaagaaaaat tggctttgct ctcggacaag cgtgacttgg
    49681 aaaatcttat aatagcaaga gatgcaactt cacgatatac aatagaaaga caaattgaac
    49741 aagtacttcg agaagtcaat ttaatggatg ttactgatga tacacaacta aaaatcgaac
    49801 ctgttaaggt tgaaaataaa atcaccgaaa agcgattgaa ggatcgggtg ctttttgatg
    49861 tccggcagtt atatgagggg gtaaacgatt cattatatcg gttagcaggt gaaaacagat
    49921 taaacgttga taagtttgct aaaaacgtta gacggatgta tgaagatgca agtgaatccc
    49981 atatatccca gagcgaaatt tactatctgc ttgttgaaac actgtttgaa aagacaggtc
    50041 gcaaatatag aggagcatgt gagattataa tctcctactt cgtacaaagg tgtgaggtgt
    50101 tcgatgagat taccaaataa agtaattccc tatactaata gtgtcattgc actcttcccg
    50161 gatattttag aggcattgac gcagcaggac atgtccccta aagaattgtt tgaaataaca
    50221 gcttttcgta aaaaggacat cgcagatttt ctaagcgctt taaactgctt atttgcatta
    50281 ggtaaaattg aattaattga ggaagggagg gtactgcgct atgttaaagg aaattcaatg
    50341 tgataaattt gctccagatc atcgagttat tcgattcaac ccaggattaa atacggtttt
    50401 aggtagtgca ggcggtagca atgcaatcgg aaaatcaaca tttctatgga ttatagatta
    50461 tgtattcggt ggtgaaagct actactccct aactgatgat ataaaaaagg aaataggccc
    50521 gcatatcatc tattttactt tcgagtttga tgaacagcca tactatttct atagaagtac
    50581 tgatgatcct aaaaatgtat ataggagcga tgaagaacgc cgtttcatta caaaattatc
    50641 actggaagat tatcggaaat ttttgtttca agaatataat attgggcttc ctgcccttac
    50701 attttcagaa ataaccgaac ggtttttccg tatctatggc cgtgaaaaca cactagaaaa
    50761 gtatccgcta cttgtcaaac ctcgtgagca ggatgaaaag gctgtagatt ttctattaaa
    50821 actattcgga cattacaaaa ttcttgcttc tataaaaagc atggaagaag aactcgggat
    50881 taagtcttct caacttaaat ctcgccatcg ccagaaggtg gacatagaga aaatcgaaag
    50941 taatcaaaaa acaatagagt cattgaggaa aaggctacaa aagctgatga aaaatagcga
    51001 agaagctcaa ttggcattgt ttggatttga cactcagacg tttgaacggg taacagcagt
    51061 acaaaaagag ttgaatagtt ttgttcgcaa gcgtaatcgt ctgcagtctc agcttaatgc
    51121 tataaagagc aatattgccg atagtaaagc cgatactgca agtgaatttg aatccttaat
    51181 acgttttttc ccaaatgcta atattaaagc atttgaagag attgaacatt tccataggaa
    51241 aattagagaa atccttggtg aagaaatgaa tcaggaaata gagcgcttac aaccgctaat
    51301 caatcagtat gataaagaaa taatgcgact taatcgaaaa attgaggaga ctggtattgc
    51361 aaaagagatg tctgagaggg tcctctctca gtgtgttaat gtgtctaaaa ctatcgacaa
    51421 acttgaggaa gaaacagcag aattgattca ccaaaaagag cttcaagaag cacgagctga
    51481 agcagaacaa acccttgaaa aactgtttca gcaacaaaat gaaaaactag atgaaataca
    51541 agatgacata aatttgcgta tggatataat taacggtgta gtaactgatc aacaggaaac
    51601 tgctcccctt ttgtatatct ctccacataa agaaatcttt tttgaaacac ctggaaacac
    51661 aagcgaggga actgcattca agagtcttat tgtgtacgac ttgagtatat tagaattacg
    51721 tcctgttcct gcgcttatac atgattctaa tatcctaaaa cgtattgaag atatccatct
    51781 agaacacata ttggaacgtt accaatctag tgggcaccaa gtttttatcg cattcgataa
    51841 ggccgattct acaactgaga aggcacacaa aattttagaa gaaacggctg tcttgcgctt
    51901 atctgacgga aatgaattat ttggtcgttc gtggagtaaa tacgaatcca atgattatta
    51961 aacataggag gaaaaaaact atggctgcaa taaatgattt actgcgtcaa attcctgaca
    52021 cttctttgcg tagtcgcttg gaacaagaat ttgctcgcat ttcaaaaaat aaaaaatttg
    52081 gacttgtatt tgaggagcat atcccagaat gcactcctct ttataatgtt cctattaaac
    52141 gcggttcaac agttgcgtta aaaactggcc acattaatga tgtatatacc gtattgaatt
    52201 tagacggtga taatgcacta tgttataaca aatcaactgg tgatacgacg aacattccac
    52261 ttgcagatct agtatcggtt gcgcagtttg gcgaaccaat attccccacg ctgcagccga
    52321 tcgcttcagt ggagaatgca cctgatagta gcttatggca tacgctcata gaagcagaca
    52381 actaccacgc cctccagtta ctggaatatc tctatcctaa gaaggttgat tgtatttata
    52441 ttgacccccc atacaacaca ggtgcgcgtg attggaaata caataatgac tatgttgatt
    52501 ctagtgataa ctggcgacac agtaagtggc tctcaatgat gcaaaaacga ttgaagatcg
    52561 caaaaagaat acttgctgat gatggtgttt tgattactac tattgatgat aatgaatatg
    52621 cgcatctatg ggttctactt catgagcttt ttccaaactt aactcatact tgcataacca
    52681 ttcagcataa tcccggtgga actcagggca aaaaattctc tgtgactcac gaatatgcga
    52741 tattttcata ttcagctgat agtactattt accgtaaaca gcatactggt ggtgatgtct
    52801 ataatctaag acgttgggga agtacttcag gccgttatga aggcgcaaca tgtttctatc
    52861 ccgtaatttt agactcgaat tataacatta tcggtttcgg tgatttactt gataaggatt
    52921 tacatccaac atcacaagta gaacacaacg aagatggtac catttatgtc tggccaattg
    52981 ataaaaacgg catagaaaag aaatggagat atggacgaga tactgtagaa tctgttagag
    53041 ataggatgta tattgaaagg cgaggagacc gaatagaagt aatccttcgt agagaaagtg
    53101 aacctcctaa aactgtctgg actgatccgt tatgtaatgc agaagcccac ggtactgata
    53161 tgatcaaggc catacttggt ggcggcttct cataccctaa atcactctat gcggtccgcg
    53221 aagcactgac attcgcagtt tctggtaaga aagatgcctt gattgttgac ttctttgcag
    53281 gtagcggtac cacattacac gcagtaaact tacttaatgc tgaggacaac ggaaaccgtc
    53341 gctgtatatt agtaactaat aacgaggtct cagatgatga atcgaaaact ctgaaaaaaa
    53401 atggctttaa gcctggtgat attgagtggg aaaaacatgg aatttgccgg gccataactt
    53461 ggcctagaac aaagtatagt atccttggaa gacgtgatga tggctctatc ttagcgggtg
    53521 agtacttcac aactcaaact gtatctgatg aagttgagcg ttcattctat cagttaagtt
    53581 ttgtagataa ccctacagag ttaacagtaa ctgccaagaa gcaagttgtt tctttacttc
    53641 gaaataagga aggaaagtca caattgccac aatcactagt aaccaaagat tgtaaattca
    53701 ttgtttcgaa taaacatacg gcatcaattc tttttgatgt aagtgcagtt gatgagtggt
    53761 tatctgccat tgaagaacag gatcaaatta cagactttta tatagtttca aaatcgacag
    53821 ctacatttaa gtcaattaag acacgtgttt cagaattgct aggccccatt attgtaacat
    53881 cacaagttaa gcgtccaatg agcgatggtt ttcctgcaaa tgctgaatac ttcaagttag
    53941 aattcctaga taaaaatctt gtttcattgg gccaacaatt ccatgaaata ctaccattac
    54001 tttggctcaa atctggagct attggaaaac gaccagaagt aaattacaat gaagagccag
    54061 aaatgttgat ccttccacaa aatagctttg ctattttagt tgatgaaaca cgattttcag
    54121 aatttgtcaa aaaactttct gaagaaagcg gtatcgaaac ggtctatttt gtaactaact
    54181 ccgaggaagc atttcgagaa atgactgccg gagtaaaagt taataacaca tatcaattat
    54241 atcgagatta tattgataac tttgtgttag gaagcaggag ggattcataa atgagagata
    54301 tattatttcc ttttcaggag acggcacttt ctgagttaca tgataaaatc aacaaggcgc
    54361 atttaatgtg gagtgaacgc gacccgcaga taatttcctt ttctgcgcct acgggttcag
    54421 gtaaaacgat aattatgaca actctttttg aagatatttt atatggaaat gcggacaaca
    54481 ttggagaacc agattctgtt ttcatttggc tttcggattc acctgagctt aacgagcaaa
    54541 caaggttgaa aatcgaaagt aaatccgata aaatccgtgt ccgtgattta gtgacaatag
    54601 actcaacctt caatgcagag tattttgagg gtggctgcat ttactttttg aacactcaaa
    54661 aacttggctc agataaactg ctgacaggta catctgataa ccggcagtat tctatatggg
    54721 agacccttac taatactgcc aaacgaattc caaaaaagtt ttatgtggta atagatgaag
    54781 cacatagggg aacttatacc tctatgcaag cagaaaataa ggcgcagtct atcatgcaaa
    54841 aattcattaa gggtagtgag gatgatggtc tttgcgttat gcctttagtt ataggggtga
    54901 ctgcaactcc tcaaagattt gatagcttaa ttgctggaac gacatcaaca gttcaaaaag
    54961 tcatcgttcc cccagagcat gtccgtgagt ctgggctttt gaaagataga atcataattc
    55021 actacccaga tattcaatta aatgctgata tgaccatgtt caaaggagcg gttgataatt
    55081 ggcgcaaaaa atgcattcac tggaagactt actgtgaacg cgaagatgaa aaaatggtaa
    55141 accctattct tgttattcaa gttgaagatg gaaatgatcg tatagcaacc caaacggatt
    55201 tgggagcttg catcgattta ctagaagaaa ctcttggacg caaattacag cctggagaag
    55261 cggtacatac ttttaatgac cgcggtacgc ttaaagtacg cgatgttgaa attcagcaaa
    55321 ttgaagcatc tcgaattcaa gacgaagaaa atgtgttggt cgtgtttttc aaaatgaatc
    55381 tttccacagg ctgggactgc ccacgagctg aaactatgat gtcattccgc agtgcccaag
    55441 attatactta cattgctcaa ttgctgggac gtatgatacg tacacctttg gcaagaagaa
    55501 tttcttccga tgctgagctt aatagtgtca gcttatttct tccatacttt gatgaagaaa
    55561 ccgtgaagaa tgtagttaat gctctacgtg atagtgaggc tgctatgcct accgaagcag
    55621 ggaccagtaa agaacttgtt acacttggac gaaatcttgc ctattctgat gtatttgagg
    55681 caatggataa cctcataact tatcggatag attcatctcg aaagcaagca ccacttaaat
    55741 tattaataca gctctctcgt gcgctgacga tggatggtat tgacttagga gcacaaaagg
    55801 ctgttaaaaa tgcagtttta tctaaaatgg atgatgagat tttacgaata aaagaaagtg
    55861 gagactatga tactcgaggg gcgtcgatta ctggttttgc tctcggtaca ctgatatttg
    55921 attatggtga taatgcttac tcctttgatg aagaaacaca gactatgacc atgtctgagt
    55981 ttgatatttc ccgacatttt gagcaagctg gaaagttgtt aggagagggc ttacacaaag
    56041 aatattggat tcgtcatagt acccgtgacc atattgatgt aaaaaaagaa atcatcattc
    56101 ttacaaatga tacagatgca atggaaagga ttaatgatta tgctgagaaa gaatttatat
    56161 cattgtacga aaacaacaag agatccattg ctagacttag cgaggcacga aagaatgtct
    56221 atgaaagatt aactaatgct tctgtacaac cgatatctgt accatggata ttaccaaatt
    56281 caatcgattt ttctgtacca gaaaatagca tgaagtttga gcagcacctt tattgttctg
    56341 aggatggcac attcgaaaca tcactaaaca catgggaaat aggagttgtt acagaagaac
    56401 tcaaaaatgg tgctgtatgt tggttacgta atcttgaccg caaaaaatgg tcacttgaaa
    56461 ttccttacga agtgggcggt gtcactactt ctatgttccc tgatttagtg attgtaagat
    56521 ctgacgcaca aggttatgtt tttgatattc tagaaccaca cgatcctagc cgcaaagaca
    56581 attaccctaa ggcagtaggt ttagcaaaat tcgcagagaa gcactgggat aaatttggca
    56641 gaatacagct tattagatta aaaaagggtg ttgatggacg cgaacatttc tatcgattag
    56701 atatgggcaa aacaacgatt aggaataaag ttcgtggcat tacatcaaac gaggagcttg
    56761 atagaatctt cgatacagac gctgtaagag aggactaaat aatacatcta tcctgtcttc
    56821 tcaaccctgt ttatttatct aacctgtcaa ctcaacccta tcactttcag ggtagttgat
    56881 atgtttcctc gaagaataaa aataagggtt tcccgacatt aacgtcctcg acaaagatgt
    56941 catgagaaaa attcattgtc aggaaaccct ttatttatca gtgttttgaa tactcttatt
    57001 tctctaccct ataagactct tataaacttc tttgtctttt tatttcatat agtactatcg
    57061 atgctgcaca agaaacattc atagatgatg ccactccatc cattggtatc tccaccaata
    57121 aatcagatat ttctttataa tttctactaa gaccaagtgt ttcatttccc aaaagtaata
    57181 ctgtaggttt taggaaatca cactgattaa ttattttatt accatgagca cttgttccaa
    57241 taatttgaaa gtttggatgt tgacttttta atgattgaat ccactcatac aactctttat
    57301 gagaaggtaa atttaacacc ggaaaactaa aaaatgtacc aaccgatgaa gctattgtct
    57361 ctgggtcata taagtcaaca gcatgtccgg ttattattaa tccattagcg ccaagtgcat
    57421 cgcaagaacg tattaaggtt cccaagtttc ccttgtttga tggtctatcc attacaacaa
    57481 ttaatgggtt ttttttagtt agttttattc tgcttaaatc attagttttc atttttacaa
    57541 tagctaataa ttctgatgta tccatttttc tgctcaattt atccattaag tgattaggta
    57601 attcatattg aaaacttacc ttaacgttgc ttagtatact cttagcccaa cccgataatt
    57661 cattttctcc tgagtaaata aaagagctta tctcccattt attcctaatg gcttcgttga
    57721 tatttctcac accttcaaca aaaaattctt tatgtttatg tcgtttgttt cggtttcttt
    57781 taatgacttc aaagtgttga aaatcattat cttctttact tatctttacc actctatttt
    57841 ttatcatatt ttaataactc cttttatttc aaaatttgca ctttaattca gcattttctt
    57901 tccaaatata tttgttaact gtaatttttt ggattttcat caagtttttc caacacattt
    57961 taaaagccaa ctcagcacct atttggttta aaaaaagcaa cttgccgttt accaagctta
    58021 aaatactcta cccgtttaaa taatttatgt tcaagttttg atttcttatc agctatcact
    58081 accatcagtg agtgattttt gtcaagggcc ttatagctat tttcgaatag attttgtatt
    58141 ggattattac taccactatt ccattctacg ttaattccat aaggtatatc agtaatgata
    58201 atatttactt ctttaatcgg aaaggcactc atatcagtaa tatcgcgttg agcacaatga
    58261 atattgatat ttgcttctcc tatcagggtt ttcaattgat ttacactttt caatgcctgt
    58321 atatgagaat cttttctaaa tacttcaata taccctttaa tttccttcat tcttctatct
    58381 aatccctctt ctgaaagcaa agaaaggttt ttctttgcta tctctaacac ctcaacatca
    58441 taatcagttg ctataagttc agatatcaaa ttatgatata gaaatccaat ggttgtaagc
    58501 aaaaaccctc ccccacaaca tggatcatat attttatatg gaccactggc tccatcttta
    58561 tcaaggatat ggaaagctct ctgaataacc tcacttgcta acctaaccgg aaatgctgtt
    58621 gtgtttgctg cattatataa aacacgtcca cttgagaaat cgctataatc tttcttaatt
    58681 gtttcaaatt tatatttcat atagtaagca cacctctctt ttatttacac caaatattat
    58741 tttttaataa tttcattatt gcatataact aacacacctt atttcttaac tacaggaatc
    58801 cagatttcac atcggaagtt tgatgaatct agatactcat ctttattgta gcagagcaac
    58861 tctggtcctt cgttaagttc atatccagac acttgcggta ctaaccattc agatgcaatt
    58921 tttgcccatg tagtttgtaa tgcttccggg aaagtacctt ctacagtaaa aacagcccat
    58981 gtagaagctg gaacggaaag cacatcccac tttccttcag gctttatagt tgttgctact
    59041 ccaatgaaat gatccaatga acttccctca aatctatcaa cctcaaaatt tacagatgcg
    59101 tttactatgc cctctagttc catattagac agttgagcca atttcctaaa atcttctttt
    59161 gaaaggctta aatacattgc cttaacatca ggattaactc ctcgatagat caatgtaatc
    59221 cttcttttta tccctactat ataaaaagct tctttttcaa caatgcgata atccatcttg
    59281 cttcctcctt ttattgtcag ttggaatgtc attggaggga tagctttcaa agcagtacca
    59341 attgtttttg cctctcttgg agtgacgcca tggaacccct gaaaggctct tgtaaaggcg
    59401 tccggtgaac tataaccata cttcatagct acatcaataa tctttacgtc agcgttgcat
    59461 aactcaagag cggcctgaga taatcgcctt tgtcgaatat attcactcaa tgtcattccg
    59521 cttagaaaag aaaacaatct tctgaagtga tactctgagc aacaagcaag ttgtgcaacc
    59581 atttgaaagt ctatttcttt tgacaaatta tcctcaatat aagtcattgc tagatttaat
    59641 tgctttaatg gtttcatatt tatacctcac tcatgttttt gttagatgtc agtaaaactg
    59701 cctgacaata aattttatca attaccatac tttttcttaa tctatgataa taaaatatct
    59761 aggattaacg agagcattca tatattctaa ataagtttct atagcccttt tctttagact
    59821 ttgcattgtt tcgtttatta atggtaaatt actctgattt aattagttct gatattgtca
    59881 atattggttt acactttttt ctcaagaatg ttcgacctta tgctgcctcc tgcaatgaat
    59941 catagtactg ctgtcgctta accatcggag gtaagccacc gttagctgaa cagattcgcc
    60001 ggttattcca atagccgatg aagtatctcc aaataagagt ttttaactgt tctactgtca
    60061 ttgagctggt atcgtaacgt ccataaagta attcctcttt gaatcttgcc cacatacttt
    60121 cacatctggc attgtcatgg catctgccac cggcactgtt catgctttga agaatgccat
    60181 atttgttgat tgccttacga tataacccgc tggtatactg cgtacctctg tcactgtgaa
    60241 gaatagctcc cctaagcata ggataggctc tataagcgtt atctaaggtt tgttcacaaa
    60301 ggggagcttt catatttgta tccatggcaa gacccaatac cgctaaatcg tagcagtcaa
    60361 agatggctga aacatacagt tttccatcag aagcttttat ttcagtcata tcagtaatac
    60421 atttttttag aggtttttca gcagtgaagt cacgttttat taaatcatct gatttccggg
    60481 cttctttgtc agctttagta ataccattag gcttgcgttt tggtttgtga ttaagaccaa
    60541 tttcgtccat gacacgataa acagttctct cgccgggaat gtgtataccc tccggctgtt
    60601 tgagttgtaa tgcctgatac atacggattc ttccataagt gtcattacat tcatcctcac
    60661 tacaaatatc aagcattgca tctgccaatt cctgatattt ccatggagca tcctttgctt
    60721 ttaggtattt attgaatgct tgtctggaaa catgaagcac tctgcaataa aaagaaattt
    60781 tgcccttaat ctcgccgtca tcagttttgg ttgcaataaa catcagtctc tgttttttgc
    60841 tgacttccga cggctggctg cgaaaaaagc actggcttct tcgagaaatt cattttcttc
    60901 ttttaaacga cggatttcct tatcctgctc tttaatgcgc tttttgagtt cgataagctc
    60961 atcgttaaga gataaggcat tcttaggcgt atgaaccgcg tcatttgcgc tgagacgacc
    61021 ttctttgaag gcttttatcc aggtgtacat agtacccttg ggaatcccta attcgttagc
    61081 tgctttatgc ccaccaattt cctgagcaag ttttacagct tgtgctttaa attcattgtc
    61141 ataagatttt tgattttgtg ccataggttg tttctcctgt tctcgtattg attatgatta
    61201 tatatcaaag tccttgagaa tagggtgtca acttttatga tacaacatca ttccatagtt
    61261 tacaaccatt atatcaaaat caaaattgga atacccgaca tattgtctat gagttttgca
    61321 gggtaatgat attaaattat tatatgtata tcaatagaaa caaagcaatt tttcctttcg
    61381 ttttacaatc atgttttcta ctggtttcat ttttaaataa tttggctttc ttttaaataa
    61441 tagaatttca ataatgtctt actcgtccat tcacagaatc cgataatatc cgtatatact
    61501 ttatcgagaa taatcctttt tattttgcta gtatggttta agagaggatg gtatcagctt
    61561 tcaaacaata gtatattcat aaaaatttga ataaactcaa taaatagaaa ggatgatgta
    61621 tcatgaagta caaactagaa aataaaggaa cttttaaagt tgttagcatc agggaaacta
    61681 taactccaaa tgaaaaaggt atttttgttc caaatatcaa ccaaacaact cgggaaaatt
    61741 tttgtcaata tttcaaacaa ttgtccgaaa gaagagtagg tgaaatacta tatatagctg
    61801 tcaaacaatg ggaagaatta aatgactatt atgtaggagt tgagataagt gaaaagttct
    61861 ccaatcctct ccctgaatct cttgtggaac tcgagatacc catgcagact tgggcagttt
    61921 ttgaactccc ctctctttca tacagcgtta ttaatgacac atggtataaa ttatttacag
    61981 agtggtttcc tgatcacgga tatgaacttg cagaaaatgt tcagtttttc agagaaaaaa
    62041 atgatattca cagatatgaa ctttgggttc cgataataaa gggtaataat aaacgattat
    62101 aatattagtg gaggatataa atttttgaca gaataaggaa taaagttcat gacattacat
    62161 caaacgagga acttgataga tatttcgaca cattagctat acgagatgat taagtataca
    62221 tctatcctgt ctcctcaact tatgcaaaat tagtattatc taatctgtca actcaaccct
    62281 attccttttt ggtagttgat atgtttccac aaacaataaa aaataagggt ttccttacat
    62341 tatctccttc ggcaaagtaa ccgtgaggaa aaccaaatgt ctggaaaccc tttatttatc
    62401 agtgttttga atagtcctat ttctccactc ttgacatcaa taccacgcac tccacatgtg
    62461 gcgtatttgg gaacatatcc atacacttaa ccatcttaat ctcataacca taccccttaa
    62521 aatcaaccaa atcaacaacc aaagacttag gattacaaga aatataaaca atctccttag
    62581 caccaaatcc acaaatatcc ctaatagcat ccttatgaat gcctggtcta ggagggtcaa
    62641 caataatcaa atcaggcttg tcctttaaat ccttaacaac cttagccaca tccccagcaa
    62701 taaactcaca attagtaagt ccattcaact tagcattctc attagcagca acaacagctt
    62761 cctcaactat ctcaatacca tacactttct tagcagcacc agccataacc tgtccaatag
    62821 acccagtacc actatacaag tcaaatacaa ctttatcatt gtaatctcca ataaaatccc
    62881 tagcaatact ataaagaacc tctgctccct tagtattggt ttggaaaaac gaaaatggtg
    62941 aaatcttaaa cttaagacct aaaatttctt cttgtatata atctcttcca tacaatactc
    63001 ttaactcatc acattggact gcatctgcta aaccatcatt aatagtatga agtacaccta
    63061 ctaactcagc ttttaaatca atccctaaaa gcatatcttt aaactcatac atatcaaaat
    63121 cttcttgaga agaagttact atattaacca taatctcatt agtatgaatc ccttttctaa
    63181 ctacaagatg tctcaaatat cctttgtgat tcattgttct ataataagga agcttttttt
    63241 cattgaaaaa ctctacacta gaaactaata tctttgagaa gtcttcatca accaacatac
    63301 actcctcaac agtttgtata tctatatgct ttcctttttt atgaagacct aatgtcaaag
    63361 gtccattttt tacttcatct ccaaaagtat attccatctt atttctataa tatttgtttt
    63421 ctggacttcc ttctatccct aaaaactgaa atccaaataa atcttgcttt aaaaataaat
    63481 ccataacttg tttttctttt atttctaatt gtttttcata tggcacagac aatagtgtac
    63541 atcctccaca ctctctaaag tgcttgcaag tttcttcagt ttcaagtggt gagttctcta
    63601 ataattctat catcttaact tcagctttct tacttctaac ttttttaact ccagctctca
    63661 ccttttgacc acttattcca cccttcatat ggataactct atctccaact tggcttaaag
    63721 atgtaccacc aaattccatt ttatctactt caaattctat aacatctttt ttcttcacag
    63781 acattcctcc aatttactat agtatcttta ttatctttct tcaatcaatt ttacttcttc
    63841 atctgttaac tctctatact cacctaactc taaactttca tccaatttta aattgcccat
    63901 agacaatctt tttaagtata ctactttttt gcctacgctt tcaaacattc tttttacttg
    63961 gtgaaactta ccttcatgta ttgtaagctc tatttctgat tcatcatcac tttttaatat
    64021 atttaactgt gatggcatag ttttatatcc gtcatcaagc actacacctt ctaaaaaagc
    64081 ttctacatct tcttcagtaa ctactccatc aatttttgca taataagtct taggcacatg
    64141 cttcttagga gataaaactc tatgagaaag ctttccatca ttagttaaaa ctaataatcc
    64201 ctctgtgtct ttatctaacc ttccaacagg aaatggttca aaagctaagt atgataaatc
    64261 aattaaatct aatactgttg ggtcatattt atctgtagtt gctgatatat atccatcagg
    64321 tttattcatc atcaaatata tatattctct atatataact tcttctccat taaataatat
    64381 ctcatcattt tcagtgtcta cttgagttcc tggatttgaa acttcacttc catttactac
    64441 tacagaccct tgtttacaat acttctttat ttcacttcta cttccataac ctaaatttga
    64501 aagaatttta tctatacgtt gctttttagc cattaaaaat gctctccatt ctcataatat
    64561 ttttcttttt caaatactgt aaatgctaaa tctatacttt ctacatctaa attatttctt
    64621 atactatcag ttattattct tgctactata tcttgtactt cttgtcctct atcaaaccaa
    64681 gctacttcaa caaaaggata tacatctgct attttaccat ctcttattgc aactgacttt
    64741 atacattcta tttcaaagta gtctcttgga cattttacag cttcaactaa atcatttatc
    64801 attttttcac ttattttaca tatatcattt tcattaattc ctcttatttt tatctgtggc
    64861 atagtttatc tcctttattt taaattttta gtaaattatc atccacttaa ttcctttcga
    64921 taacgtttga agtggtaact tcttggtcaa ggcaactatt gttatcagta tctccaaact
    64981 aataaggtag ttcctaccct tatagttagc attatatagc tagttttcct aaattgttta
    65041 tattctttat tattttattt aatcctattg tcttctattt aacattatac ttgtaattta
    65101 tagttattac aatttactat atactttttt tgatttaacg ttataatata agtgatgggt
    65161 aaattttaaa agttaatagg aggagtttaa aatggcatta attactacaa aagaaatgtt
    65221 taaaaaagct tacgaaggtg gatttgctat aggtgcattt aacataagtg acttagaaca
    65281 attacaaggt gttttaaaag ctgctaaagc taaaaattct tatgttatga tacaagcttc
    65341 tatgtcagct gttaaatatg ctggacctca tacattagtt gaaatggtta aggctgcttc
    65401 tgatgaaata ggtgtagatg tagctcttca cttagaccat ggtccaaata tggatgctat
    65461 taaaacttgt attgatgctg gtttctcttc agttatgata gatggttctc attttgactt
    65521 tgaagaaaat gttagaataa ctaaagaagc tgttgaatat gcacactcta aaggtgttgt
    65581 tgttgaagca gaacttggtg ttctagctgg aacagaagat gatgttactt ctgatgttca
    65641 caagtatact caaccagctg aggcagttga gttcgttgaa agaacaggtg ttgattcatt
    65701 agcaatagct attggaactt ctcatggtgc tttcaaattc aaaggagaag ctaaattaag
    65761 attcgatata ctagaagaaa tccaaagcaa attaccaggt ttcccaatag ttcttcatgg
    65821 tgcatctgct gttgaccaaa acgctgttgc tacatgtaat gaatttggtg gaaatatagc
    65881 tggtgctaaa ggtgtacctg tagatatgtt aagaaaagca tcttctatgg ctgtttgtaa
    65941 aataaacatg gatactgact taagactagc tatgactgct gctatcagaa aattcttagc
    66001 tgaaaatcca aaagaatttg acccaagaaa atacattggt gctggtagag atgctataca
    66061 agctgtagtt gaaagcaaga tagatgatgt tttaggttct gctaactcta taaactaata
    66121 taacttttaa atatttataa aagaaaagag atgacttaaa gtaattaaat ttatttttaa
    66181 gccatctctt ttaagttgta taaattatgt atcttataat attttaatta tattgtcttt
    66241 ttaaactgac agctaaacgt gaatatgtcc actctttatc tttctatgga tggtagaagg
    66301 atatatacct ataacttcag cagcctttgt tattgaacca tacttattta tagccttact
    66361 tatcattatt tggtccacta ttttatatgc atcattaaga ttcataattc catttaccac
    66421 tattggtgat aaatcatctg tttcattatc aatcatatct aaatttatca gcatgttaaa
    66481 ttcatcttct ccaattacat ttttagctga taatactaca aatctttcaa tgatgttttt
    66541 taattctctt atgtttcctg gccaagaata ttcttctaat aactcaataa ctttaggcga
    66601 aacttctact tctctattgt acttttcatt gtaaagtttt aagaaataat ttactaatgg
    66661 tactatatcc tcttttcttt ctctaagagg aggtattttt actggaataa cacttaatct
    66721 atagtacaaa tcttgtctaa atttaaggtt atttctaagg tcttcatgag aaatattagt
    66781 agcacttata tatctaacat ctatttttat aggcttacta ccacctattc tagtaatagt
    66841 attttcttgt ataaccctaa gtaatttttt ttgcatctgc attggtaact cacctatctc
    66901 atccaagaaa atagttccac cattcgcctc ttcaaaaagt ccctttttac cttttttctt
    66961 agctccagta aaagaacctt cttcatatcc aaaaaactct gactctaata attcatttgg
    67021 tatagctcca cagtttatag ctataaaagg tttgtctctc cttgaactat acttatatat
    67081 tgttcttgct attatttctt ttcctacacc tgattctcca tttataaata tagaagaatc
    67141 tgaggctgca gctctcttaa caactttttt tatattgtcc ataatcttac tttgacttat
    67201 atactctgtg tcttctgata tatcactata ttttatctga gattcaagct taataaccct
    67261 tgaaatatct ctccctgtta gtactcctcc tgtaagcttt cccttagaat cccatcttgg
    67321 aactgccgta tatgcaataa gcttcccatc tgggtaaacg atatttctat aaatcttttt
    67381 cttcacttta agcatgttta atattattgg ttcttcaact aatccttgtc tcacaaggtc
    67441 tgttatattt ctaccaagta cctcagttct tttaaaaggt aatatctcat cactgacagt
    67501 gttcatcttt tcaattaccc catctttgtt aaacacaaca acaacttcat ctatatgctc
    67561 tattatatct tcaattactt tatcagatat tagagacttc tcaaataaat aaactatatt
    67621 attttcatta ttattatctt tcatattgta tatatgtgca gtcatcctta actctctgaa
    67681 agctattttc tttttactat ctgtcagtat ttctgttttg tcaaaatttg aatctaattc
    67741 atttatattt ctaggctttt ttatatctag aaaactaaaa atactatcta ttagatgatt
    67801 acagtatatt atttcgcctg attctttaca taaaatggct ggattttcta catgttctat
    67861 aagccctatt aaattattat ccattaatat tcgcaccttt cattaagtaa tttattgaac
    67921 tacatacaaa atttattgtt tgcttacaat aaataccatc agcacatata atcaactcct
    67981 tataatattc gtcttttcaa gtagtttatt tcattaaaac gtaactaaga tataatttta
    68041 atgattatag tttgttaaaa atttcatttt gcttttaatt gtattttatc acatatttag
    68101 taattatttg tctatttaat acaatttaat taaatttaag atttatttaa aaaaacaaag
    68161 ataatcctta cagaatataa ctcacaattt gtaagcaata tacatataag gattatccag
    68221 attatcttgt attataaaat tttattcaaa cagacttttt aataactgac aaatacacac
    68281 tagcaataac ttatcaaagg gttaataatt atgttactaa ctaataagac ttattcaatc
    68341 agtaatattt atatagtttg tatctattaa gcttttatag cttctaataa atcatcaacg
    68401 ctatctaatt tttctataag cattggaact atttcatgta agtctccaac tactccatag
    68461 tcagcgattt ctaaaattgg agctgctggg tttttgttta tagcaattat gaaatctgat
    68521 gattgcatac ctgctaaatg ttgtattgct cctgatatac cacaagctat atataggttt
    68581 ggtttaacag tagttcctgt ttgacctact tggtgagaat ggtctatcca tccagcatca
    68641 acagccgcac gagaagaacc aactactcca cctaatttgt cagctaattt cttaagcatt
    68701 tcaaatcctt ctggtccacc taatcctaga ccacctgata ctataacatt tgcatctgta
    68761 agagatacta aatctttctt agttttaact gtttctaaaa cagtagttct tatatcatct
    68821 tgagttattt tgtagtctaa tgctataact tcaccagttc ttgtttcgtc tttttcagct
    68881 ttatccataa caccaggtct aactgtagac atttgaggtc tatggtttgg acaaatgatt
    68941 gtagccatta tgtttccacc aaatgctgga cgagtttgtt ttattttctt atcttctggg
    69001 tctatttcta acttagtaca gtcagctgtt aatccagttc caactcttga agctattcta
    69061 ggtgctaagt ctctacctat atgagttgct ccgaaaagca ttatttctgg ttttatttca
    69121 tctattgcat cttttattac ttttgtatat gcatcagttg tatatttttc taaaagagca
    69181 tcatctgcaa cataaacttt gtcagctcca aaagtgatta attctttagc taatccatca
    69241 acatcttttc ctaataatat tgcacaaagt tctgcatcta cttctttagc taattttctt
    69301 ccttctccta ataattctat aactactgga gctatttttc cttctctttg ttctgcaaat
    69361 atccatacgt ttttataaga acttaaatct tttatatcat tcatagtttg tactcctcca
    69421 acttatataa tgtgcttttg ttttagaccg attattattt tactagctat ttcatcagct
    69481 ttaccttcta agatttctcc ttttcctctt ggttcaggag taaatgatct aaatacttta
    69541 gttggtgatg cttttaaacc aacttcttct ttatttacat ctaaatcatc aattgtccaa
    69601 acttttacat catgtttata tgcttttact attttatcaa cagacatgta tctaggtgta
    69661 tttaactctt ttactgctgt taaaagtaca ggtgtactta cttttattaa ttcgtatcca
    69721 tcttctaatt gtctttgaac tattatatct tttccttcta ttttgaaatc ttgtacatat
    69781 gtaacttgag gaatgtctaa tttctctgca atttgtggtc caacttgtgc agtatctcca
    69841 tctattgctt gtcttcctgc aaatattatg tcatagtcac ctactttaga tattcccgct
    69901 gcaatagtat ttgatgtagc ccaagtatca gctcctccaa atgctctatc acttaataat
    69961 attgcatcat cagcacccat agctaaacac tctcttagca taaagtctgc ttgaggaggt
    70021 cccattgtta taactgttat agttacatca tcatgctcat ctttaatttt taaagcttcc
    70081 tctaatgcat ttgcatcatc tgggtttaat atacttggaa ctccatctct tataagagta
    70141 cctgtttctt tatttattct tacttcgtta gtatctggaa cttgttttac gcaaactaat
    70201 attttcaaag tctatacctc caatttcatt agattattta ttattttaat acgtttgatg
    70261 aaattaccat tttttgaact tctgatgttc cttcgtatat tgatacgatt ctagcatctc
    70321 tgtacattct ttcgatttca taatctttta taaatccata tccaccgtga agttgtaatg
    70381 ctttataagc aacttcattt gcaatttctg atgcatagta tttagccata gcagattctt
    70441 gagcaactct cattcctgca tctctctttt gtgctgcatc ataaactaat cctctagctg
    70501 cacaaacttt tgtttccatg tcagctatag taaattgagt attttggaat ttagctatag
    70561 gcttaccaaa ttgaactctt tgttttgtat atttaacagc ttcatctaaa gcaccttgag
    70621 ctattcctag agctaaggca gcaacaccta ttctaccaac ttctagagta cccattgcta
    70681 tcttaaatcc ttgaccttct tttccaagta agttttcttt tggaacttta acgttttcaa
    70741 atattaagtc agaagtttca gttcctctta tacccatctt atcttcatga gctcctgttg
    70801 agaaaccatc ccatttactt tctactataa acgctgatgt tcctctaagt cctttacttc
    70861 tatctgtaac tgctattact atagctacat cacagaatgg tccattagtt atgaaagttt
    70921 ttctaccatt taatatatag tggtctccaa ctaattctgc tgttgtttgt tgtccacctg
    70981 catcagaacc agctcctggc tcagttaaac caaatgctcc tattaattca cctgttgcta
    71041 taccttttaa gtatttttgt ttttgctctt cagtaccatt gtaaagtatt ggtaatgaag
    71101 caagagaaga accagctgta acaaaagtag ctgttgaagc acatcttcta gctatttctt
    71161 ccattactat agcgtaagaa acatatcctg ctcccccacc accatattct tctgggatat
    71221 tactgcttat aaattttgca tcagctaatt ttttgattaa ttccttaggc attacacctg
    71281 ttttatccat ttctgctggt aaagtatcta attctttact aacaaaatct cttactgctt
    71341 ttcttaaaag ttcttgttct ttgttatata acatagcttc ctccaatttt catattttat
    71401 agtaaattta ttttaatgaa cttacgctaa actcatcatc tcagcaaaag tttgaatacg
    71461 agttctggct tgttcattat tctgagtttg ttggtcgatt tcaacatata aagtaggtat
    71521 tccactatct tctatatctt ttctaactaa aggataatcg tattcttctg ggtcacagaa
    71581 tttcatcata cagaagataa caccatctat atctttcttt ttaacttcat ctactataag
    71641 tgacccacgt tttttcttag ggtcataagc taatgaacat ccttctatgt ttgaccattg
    71701 tcttgctaat ctttctaacg catcatcacc tgctggtaca tctgttctga attgtcttgt
    71761 ttcttgtgct aagtcgtcag ctacaactga tatattgttg tcttctaaaa tgtctaatat
    71821 atctttagaa tcagctaata tacctgttaa taaaactttc tttccagaac agacttcttc
    71881 tggcatagca tttaattttg ctattaaatc tttcactagc tcagtatgtt cttcttttct
    71941 cataaagaac ccactcttaa ttactaagct tcttattgat ggttttatag tattagaatg
    72001 tttataagct acttcaacaa agtctctcat agtttttcta tgttcattgt aaacttctat
    72061 actttcatga atttttgctt cttctatttc atatccacaa atttcttcaa gttctctttt
    72121 tacgcctttg tactcactga ttaagtattt tacaccagct tcaagttttc tattttgtgg
    72181 gtgtactaat gatatatatt ttatatgagg tactgctgat ttccagtttt gtcctaaaca
    72241 aattagtgta tcacacatac ctggtataat aactccagat aattcatcat aagcaccttt
    72301 taatccatat tctaaacatg attgcattat tgaacatgca aatgcaggga aatattgttt
    72361 agctaaatct aattctgttt gccctcccca tataccaact ggaagcattc cagctgcatg
    72421 tataatttct tctgggcaat aaactgggaa acaacctata gcttttttac cagtttcact
    72481 tttatatttt tttacagccg catttggatt ttcaactact tctttcattt tagataaaat
    72541 agcttccatt atatctcacc tctattaagt ttttttcttt cttccattac ttcaactaaa
    72601 ccttgaattc ttgtttcaaa ttgagcatta gtgaaagctc tagggtctgc ttggtcacca
    72661 tcaaatccag catatggtaa tccagtttct tcagctgctc ttctttgcat ttcatattgt
    72721 attaaactca taagtttaca gcttctgttc atatgataga atgctccatc acatttaccc
    72781 tctactaaag aatcaactct atattttgtc atacggtcta ggtttacatt gttaaacata
    72841 gtactatatg ctactgccat tccatctaaa tcattaactt catattgtaa tgcccaagca
    72901 tgtgggtaaa cactacctgt catgttaact ccaaatttag ctaatgtttt catcttgtat
    72961 cctatatatg gccaacaagg tataccttcc atcattattc tgtatttttc ttcccctctg
    73021 aaagaagatt taccagtttt catgttgtcc tctaattctt ctataagtaa cttaaatgct
    73081 tctgtagttt cttttttacc tctagcacaa actattacag ccatgtaagt aaataagtca
    73141 aatccattca ttggagaagg agaagaatct gctggtaaac tcatagaata cttccatagt
    73201 cttccgtttt cagctgatat tttcattact tcttcaaact tcttagggtc aaatttcttt
    73261 cctgatataa tttctagttg ttttatagct tcttcaaatt gagctttaat ataatctatt
    73321 cttgattgag taacttcgtc ttcattattg aaagttgtat caatcattat taaaggtata
    73381 tctaattctc ttgaaatatt ttcataccat tttataactt ggttacatat attgttacag
    73441 caaagtagga aatctggagc tggcatatcc aaagcttcac atccaccatt ttctaaaaga
    73501 ccaaaatttg ttcttgcata tgcacatagg tcaatagaat atcctttaga ttcagctatt
    73561 tcacataatt ctaaagaacc ttttttagct gctactccag ctgcttggtt ttctggatat
    73621 aatacgttta agtcaaatac ttctgctaac tcttgaggaa atactgatgt tgaccaacct
    73681 acaggtcttc cttcttcttt agctttaaat gcatttgcat attgttcagc taataaatca
    73741 ttaattacta ctctagcttc ttttttttca gacattttca tccctctttt tatatatttt
    73801 tcacttcttt ttgtgattct tttgcttcat caaaagcata taacgctgct cctaaagcac
    73861 cagttaattg aggtatatca ggtacaataa tttctgtgtt gatttctctt gccatagctc
    73921 ttacaatacc actatttcta gcaactccac caaccataac tacatttctt tgtactccta
    73981 ttctttttac taggctagaa actctctttg ctactgaagt atgaatacct gccactatat
    74041 cttcaatttt tgcattttca gataaatgtg atataacctc agactctgca aatactgtac
    74101 atgtactgct tattgatact tcattttgag aattcataga tatacttccg agttcagata
    74161 catcaacctc tattattttt gccattacat ctaaaaatct tcctgtacct gcagcacatt
    74221 tgtcattcat aagaaagttt aatagtcttc cattattatc taattttaat acctttgcat
    74281 cttgaccacc tatgtcaata atggttcttg tctctggaat tatgaaattt acccctctag
    74341 catgacagct taattcactt atttgcttat cagcatctga atagttcatt cttccatatc
    74401 ctgtaactac aactttttta atatcttctc ttgcaagacc tgtcttgcca tataattttt
    74461 ctaaaactct tgatggtcca gtagtcccag taccagagga tattgtttca gaagctacaa
    74521 tatcttcccc attctttaag attactccct ttgatgcagt tgaacctata tctaatccca
    74581 ttgtgtacat ctttagcatt ctccttttaa aaaatctaga attactaatt aatatcttac
    74641 aactttacta tctttaaagt tatttatttt ctcttcatcg tagcctaaag attttaatac
    74701 ttctacagta tgttgaccta cttttggtgc tggtgtatat tctttaattc cttcatttct
    74761 aaacataact ggagtattaa ctaagacacc tgtatttccg ctatcgtatg ttttcttaaa
    74821 taagaagtca tttgcccaag cttgttcgtc atctaataaa tcttcacagc tttgaatttt
    74881 ttcaaatggt aagtctgctt cttctaataa agctgaccac tcgtctaatg ttttttctaa
    74941 catagcttct ccaactatct taactaaatc ttcaacatga ttaaccattg aatctatgtt
    75001 attatatcta tcatcttcta atatatattc tctatttata accttacaga atttgcctaa
    75061 ccacttgttg tattgtatta aagctaattg aatccatctt ccatctttac atttatatgt
    75121 agtcattaat gggctgtttg gattttctct tgataaaggc atttcatttc cgtattgtgc
    75181 tgttgttatc attgttccca ttccatatat agctgtatgg aaaagactta ctgttactct
    75241 ctcaccttta ccagtttgag cttttttatg taatgctgct aaacttcctg ctgctagtgc
    75301 tagacctgca tagtggtcac caaatcctgc tgctgtattt gctggagatg ttcctttttc
    75361 cataacagat tggctaactc ctcctcttgc gaagtatgca gtatagtcaa atcctggttt
    75421 atcttttaaa ggtccttttt caccatatcc taatatttga gagaatatta atcctggata
    75481 cttatctttt atttggtcat aagctatacc cattttttct aatgcttgaa ctctaacatt
    75541 agttacaaat atgtctgctt ctgataataa tttatgtaat atttctactc cttcttttga
    75601 ttttacatta atacttacac cctttttatt tccattttct aattcaaaca tagggttttc
    75661 atcatctgat gctggagatt taaatgttcc acccataact cttattccat caccttctat
    75721 aggttcaatc ttaataacct ctgcacccca gtcacctaac atttttgcac aacatggtgc
    75781 tgcgatgaaa cttgaaagtt ctactacttt aactccttct aaaagcataa cgttttcctc
    75841 cttaatagta tcaattataa aatttcgatt aaatcactca taaaatactt ttttctgata
    75901 ttaactgtct gatgtcaaag ttcctaatct tatctaaaat aacttaaaaa ttattaaaat
    75961 tattttttat tgttttattg tcttacacct atattgttaa gcatttatcg tgccaacttt
    76021 atatttaatc aaaatatcgc ttgatttgaa tgttttttaa caatatgcaa ttgaataatc
    76081 tttttcatat cgtggcatct tgccactatc cattgcattt tgctattctt tctatgctta
    76141 taaaagtttt ttttatatat ttttttatga aaaagcttga catttcaaaa aaagttttca
    76201 ttgctaaact caaaaacac
//
LOCUS       DAGSHT010000002        66237 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGSHT010000002 DAGSHT010000000
VERSION     DAGSHT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 66237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.003"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            235..999
                     /locus_tag="MJB72_000079"
     CDS             235..999
                     /locus_tag="MJB72_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY8261260.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            1388..2701
                     /gene="prdC"
                     /locus_tag="MJB72_000080"
     CDS             1388..2701
                     /gene="prdC"
                     /locus_tag="MJB72_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBY8261261.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      3104..3189
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            3771..5846
                     /locus_tag="MJB72_000081"
     CDS             3771..5846
                     /locus_tag="MJB72_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBY8261262.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPGGEKPSDSQVPDGEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQD
                     IDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNS
                     CLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCY
                     NNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEEN
                     ELMTYSATQSAIWIILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVA
                     INIKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGV
                     VKLRLPDGVTAYDGDGNIIGTGEVEISTQQKFKLKSVVKPDAKANISAVSYDYIFPKA
                     IQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            6253..8013
                     /gene="prdR"
                     /locus_tag="MJB72_000082"
     CDS             6253..8013
                     /gene="prdR"
                     /locus_tag="MJB72_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBY8261263.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            8442..10322
                     /gene="prdA"
                     /locus_tag="MJB72_000083"
     CDS             8442..10322
                     /gene="prdA"
                     /locus_tag="MJB72_000083"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBY8261264.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            10403..10687
                     /locus_tag="MJB72_000084"
     CDS             10403..10687
                     /locus_tag="MJB72_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261265.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            10725..11450
                     /gene="prdB"
                     /locus_tag="MJB72_000085"
     CDS             10725..11450
                     /gene="prdB"
                     /locus_tag="MJB72_000085"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:11175..11177,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBY8261266.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            11530..12288
                     /gene="prdD"
                     /locus_tag="MJB72_000086"
     CDS             11530..12288
                     /gene="prdD"
                     /locus_tag="MJB72_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBY8261267.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            12303..12770
                     /locus_tag="MJB72_000087"
     CDS             12303..12770
                     /locus_tag="MJB72_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBY8261268.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            12788..13270
                     /locus_tag="MJB72_000088"
     CDS             12788..13270
                     /locus_tag="MJB72_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBY8261269.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            13290..14297
                     /locus_tag="MJB72_000089"
     CDS             13290..14297
                     /locus_tag="MJB72_000089"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBY8261270.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            14417..15298
                     /locus_tag="MJB72_000090"
     CDS             14417..15298
                     /locus_tag="MJB72_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBY8261271.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            15966..16601
                     /locus_tag="MJB72_000091"
     CDS             15966..16601
                     /locus_tag="MJB72_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY8261272.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            16785..17354
                     /locus_tag="MJB72_000092"
     CDS             16785..17354
                     /locus_tag="MJB72_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY8261273.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            17432..17569
                     /locus_tag="MJB72_000093"
     CDS             17432..17569
                     /locus_tag="MJB72_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261274.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            17566..19341
                     /locus_tag="MJB72_000094"
     CDS             17566..19341
                     /locus_tag="MJB72_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261275.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            19766..21073
                     /locus_tag="MJB72_000095"
     CDS             19766..21073
                     /locus_tag="MJB72_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBY8261276.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            21401..21940
                     /gene="hpt"
                     /locus_tag="MJB72_000096"
     CDS             21401..21940
                     /gene="hpt"
                     /locus_tag="MJB72_000096"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBY8261277.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            22318..23994
                     /gene="bclA2"
                     /locus_tag="MJB72_000097"
     CDS             22318..23994
                     /gene="bclA2"
                     /locus_tag="MJB72_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBY8261278.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGAVGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLATNNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(24500..25255)
                     /locus_tag="MJB72_000098"
     CDS             complement(24500..25255)
                     /locus_tag="MJB72_000098"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBY8261279.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVAEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKSNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            25695..26684
                     /locus_tag="MJB72_000099"
     CDS             25695..26684
                     /locus_tag="MJB72_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBY8261280.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSSGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(27136..27852)
                     /gene="dapD"
                     /locus_tag="MJB72_000100"
     CDS             complement(27136..27852)
                     /gene="dapD"
                     /locus_tag="MJB72_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBY8261281.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(28112..28861)
                     /locus_tag="MJB72_000101"
     CDS             complement(28112..28861)
                     /locus_tag="MJB72_000101"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBY8261282.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(28928..29815)
                     /locus_tag="MJB72_000102"
     CDS             complement(28928..29815)
                     /locus_tag="MJB72_000102"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY8261283.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(29932..30933)
                     /locus_tag="MJB72_000103"
     CDS             complement(29932..30933)
                     /locus_tag="MJB72_000103"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBY8261284.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(31353..32234)
                     /locus_tag="MJB72_000104"
     CDS             complement(31353..32234)
                     /locus_tag="MJB72_000104"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY8261285.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            32557..33750
                     /locus_tag="MJB72_000105"
     CDS             32557..33750
                     /locus_tag="MJB72_000105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBY8261286.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            33956..35074
                     /locus_tag="MJB72_000106"
     CDS             33956..35074
                     /locus_tag="MJB72_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBY8261287.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKDLIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            35205..35450
                     /locus_tag="MJB72_000107"
     CDS             35205..35450
                     /locus_tag="MJB72_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBY8261288.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            35437..36183
                     /locus_tag="MJB72_000108"
     CDS             35437..36183
                     /locus_tag="MJB72_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY8261289.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            36468..37343
                     /gene="hslO"
                     /locus_tag="MJB72_000109"
     CDS             36468..37343
                     /gene="hslO"
                     /locus_tag="MJB72_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBY8261290.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            37734..38534
                     /locus_tag="MJB72_000110"
     CDS             37734..38534
                     /locus_tag="MJB72_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY8261291.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            39062..41302
                     /locus_tag="MJB72_000111"
     CDS             39062..41302
                     /locus_tag="MJB72_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY8261292.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYKSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            41716..43251
                     /locus_tag="MJB72_000112"
     CDS             41716..43251
                     /locus_tag="MJB72_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY8261293.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVTIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            43267..44028
                     /locus_tag="MJB72_000113"
     CDS             43267..44028
                     /locus_tag="MJB72_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261294.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGAFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            44366..45235
                     /locus_tag="MJB72_000114"
     CDS             44366..45235
                     /locus_tag="MJB72_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8261295.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            45564..45815
                     /locus_tag="MJB72_000115"
     CDS             45564..45815
                     /locus_tag="MJB72_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBY8261296.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            45927..46706
                     /locus_tag="MJB72_000116"
     CDS             45927..46706
                     /locus_tag="MJB72_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8261297.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVVSQKNEYTDLQQDLVGATWILQKE"
     gene            complement(46841..47491)
                     /locus_tag="MJB72_000117"
     CDS             complement(46841..47491)
                     /locus_tag="MJB72_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261298.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGSKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(47488..47991)
                     /locus_tag="MJB72_000118"
     CDS             complement(47488..47991)
                     /locus_tag="MJB72_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8261299.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(48040..48555)
                     /locus_tag="MJB72_000119"
     CDS             complement(48040..48555)
                     /locus_tag="MJB72_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY8261300.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            48835..49362
                     /locus_tag="MJB72_000120"
     CDS             48835..49362
                     /locus_tag="MJB72_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8261301.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            49346..50749
                     /locus_tag="MJB72_000121"
     CDS             49346..50749
                     /locus_tag="MJB72_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8261302.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(51132..51443)
                     /locus_tag="MJB72_000122"
     CDS             complement(51132..51443)
                     /locus_tag="MJB72_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261303.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(51499..52092)
                     /locus_tag="MJB72_000123"
     CDS             complement(51499..52092)
                     /locus_tag="MJB72_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY8261304.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            52943..53593
                     /locus_tag="MJB72_000124"
     CDS             52943..53593
                     /locus_tag="MJB72_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBY8261305.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            53807..54478
                     /locus_tag="MJB72_000125"
     CDS             53807..54478
                     /locus_tag="MJB72_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8261306.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            54475..55605
                     /locus_tag="MJB72_000126"
     CDS             54475..55605
                     /locus_tag="MJB72_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8261307.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            56025..56870
                     /locus_tag="MJB72_000127"
     CDS             56025..56870
                     /locus_tag="MJB72_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261308.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            56870..57898
                     /locus_tag="MJB72_000128"
     CDS             56870..57898
                     /locus_tag="MJB72_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8261309.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            57901..58914
                     /locus_tag="MJB72_000129"
     CDS             57901..58914
                     /locus_tag="MJB72_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8261310.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKEYGTVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGNTLQDIFRIKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIVMLLLVVFSAFISSLIGLFVGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGIN
                     INGIGNLCYIMPSVATFNGIMEIINCNAIVIKDIVILAVHCVAWFMLYMILDKKRKLS
                     FSR"
     gene            complement(59433..60683)
                     /gene="cme"
                     /locus_tag="MJB72_000130"
     CDS             complement(59433..60683)
                     /gene="cme"
                     /locus_tag="MJB72_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBY8261311.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDAIIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINGSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLSILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMQGRILSLPIIIYTGFIPLGMVIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGIFKSNESE"
     gene            complement(60769..61515)
                     /locus_tag="MJB72_000131"
     CDS             complement(60769..61515)
                     /locus_tag="MJB72_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8261312.1"
                     /translation="MNTYTTSEVADLIGVHPNTVRLYEELNLISKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAICLINQYLQQLEIEKSN
                     AEEALTIVSQILNGNTPETDKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(61630..62652)
                     /locus_tag="MJB72_000132"
     CDS             complement(61630..62652)
                     /locus_tag="MJB72_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBY8261313.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IVRKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMNESLERLCKQKDWIKYTLNQLGQKGEIG
                     AFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDLFGKN
                     LKGWAKDRQGNSTGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNGN
                     EYGYLWLLCKKDGVLWYSAMGTGGNAICCIPKYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(62652..65072)
                     /locus_tag="MJB72_000133"
     CDS             complement(62652..65072)
                     /locus_tag="MJB72_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY8261314.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYVGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSEAVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRVKLAKEIGKKRNGNILYVLDEPTTGLSLYDTAK
                     LIQLLDELVLNGNSVVVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKALKENP
                     KSITGRYL"
     gene            complement(65158..66078)
                     /locus_tag="MJB72_000134"
     CDS             complement(65158..66078)
                     /locus_tag="MJB72_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8261315.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRTFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYTSEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKDNDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPYNHPQNRHLVDIYLPIEPF"
BASE COUNT        25030 a         8089 c        11477 g        21641 t
ORIGIN      
        1 gtgatgttaa tactaatttc tccaaaataa aaataatata tacagaaaaa taaaggtaaa
       61 aatgtaaaaa atataaaaaa attaaataaa tgagtatttt agagagttta attaggtcaa
      121 tataggtttt atttttttag ttattgtctt ataaattaaa tattacagta cttttttgag
      181 ctgaaaaaaa ataaatttta aatatagaag aaattatttc aaagggggaa tagtatgaaa
      241 agagaagtta aaaggactaa agctttgata tatagtgttt tatttttttc attattatta
      301 gtgttaatat ttgtttttgt ttatggaata aaatacatat gttctaaatc tgacaattca
      361 aaaatgccta tatataaagt tgatacaaat gagaaaaaaa tagctttgag ttttgatgta
      421 gcatggggaa ctgataatat ggatgatatt ttaaaaattt tagataaaca taatgtaaaa
      481 gctaccttct ttttagttgg tagttgggtg gatgataatg aagaaattgt aaaagctata
      541 gatgaaaaag gacatgaaat tggaaatcat tcaaatactc atgccaattt gaaggaaata
      601 tcaaaagaag acataacaaa agagatagaa actacatcag aaaaaatata taatataaca
      661 ggaaaaagga ctaatttatt tagacctcct tttggggacg taaacaataa agctatggac
      721 atttgtagtg atttaggtta taaagtaatt aagtgggatg tagattctat agactggaag
      781 gagcttggac caaatcatgt aattgaaaaa gtaataaaag aatctcaacc tggttcaata
      841 gttttattcc atgctaatat aaatgatgta gataactatt tggatactat aataagtcga
      901 ctaaaaaaag atggatacag tctagtcaaa atatctgatt tgttgtataa agataattat
      961 gtagttgatt ccaatggagt gcaaaaaact aagaattaat aagtaataaa tgtatatttt
     1021 aagtaaagtt gtgataaatt atatagtata tgttagaaaa gtgtcgataa aaagacggtg
     1081 tctatttttt gacacttttt atacatgtat gcaatatcaa taattataga ttaaattgat
     1141 taaaaaagtg tctaataatt gacttttttt tgatgggttt aaaaatttag aagaattaat
     1201 gaatgctgta agacagtatt tccaaagggt ataagtttgt ttaaaagttg gcatagaaat
     1261 tgctttgtta ataagcgata tgttctatag aaataaacaa tatgttaaga ccttataata
     1321 gaaaaatagc attaacatta tttgaacagc ttataaaaaa attaatatac ttaagggggc
     1381 gtactagttg aacaaactat attctatttt attaaaacaa cacgttggtg gtccagataa
     1441 gccagtagta agtgttggag atgtagtaaa aaaaggaact ctaatcgcag aaccaactgg
     1501 attaggggca aatatttatg ccagtgttag tggaaaaata agtgaaataa atgaccaagc
     1561 gatagtaatt gaagctgatg aagtacaaga agatactttt gaaccattaa aaggagaagg
     1621 tatacttgac ttaattaaag aagctggagt agtaggaatg ggtggagcag gattccctac
     1681 tcatgtaaaa ttaaacatag atttaaatgg tggaacaata ttagcaaatg cagctgaatg
     1741 tgaaccttta ttagctcata atataaaaga aatagaagaa agaccagaaa tagtttatca
     1801 aggtataaaa tatgctatgg aagttactaa tgctggaaaa ggtatgctag caataaaaag
     1861 taagcatcca aaagcaatag aagctttcaa aaaagtaata aagccaggag acaacataga
     1921 agttgcagaa ctagttgata tgtacccaat gggagaagag agagcaatag taagagacgt
     1981 tctaggaaaa ttacttgagc caactcaatt accttcagag gctaatgcag tagtaataaa
     2041 cgtagaaaca ctaactagaa tagtagaggc tgttgagcaa aaaagaccag ttatatctaa
     2101 aaatataact gtagttggtc aattaaacag tggaaaagaa tctatagtgt ttgaagatgt
     2161 gccaattgga acaactgtcg gagaattaat tgaaagagct ggtggaataa aaggtgaata
     2221 tggtgaaata atcctaggag gacctttcac tggtaaagct actactttag atgctccaat
     2281 aactaaaact agtggtggta taatagttac tatgccattt gttaatgaaa agagaaaaat
     2341 gggtctttta gtttgtgcat gtggacctaa tgaagaaaga atgagagata tagcaactaa
     2401 aatgggagtt actgatatag tatcagttca aaaatgtaaa caagctcaag aaattaaagg
     2461 tgcattaaaa tgtgaaaatc caggacactg tccaggacaa gctcaaaaat gtattgaatt
     2521 taagaaagct ggagcagaag ttatattaat aggaaactgt actgactgta gtaacacagt
     2581 aatgggttct gcaccaaaat taaaattagg tgtatatcat attactgacc atgtaatgag
     2641 aacagttaat cacccattaa taagaagaat aaaaggtgac gtaagattaa aaataaaata
     2701 atagataaat agaagttaga catctttaga agtaaaactt aaaagttact tctaaagatg
     2761 ttttttaatg tattttgagg gcaatatata ttgtaaaata aatggaaaaa atagacaaaa
     2821 tatgctagat attgtataat gataacaata gctattacaa ttaatattgg acaaaaataa
     2881 tgatactcaa gtatgtggtt tgtattaata tgcatattat attaagtcaa ctatccgata
     2941 gagtaaaagg tttttgtgat atttgaagat actctatagt atctttttta ttataaaaac
     3001 taatttttgt cttttattag ataagtatct aggatactaa tttatatttt attgagttgt
     3061 taaaaaagtg cacttaaaaa gttaaaaaag aaaaattaaa aaacctattt atagaaactg
     3121 tgaagtatat cttaaacctg ggcacttaaa agatatatgg agttagtagt gcaacctgct
     3181 ataaatataa ataggagtaa cttttaattg tcaaaaatat ataaaataaa tacataaata
     3241 agtaattaat agaagataaa tataaatata tttttgaaat gctccctgca ttcataacga
     3301 gtgtagagtg gcagatatct gcccaaaact cctttaattg ctggaagttc ctaaagctaa
     3361 ctaaaccaca acataatcat gaaataagga taagtgtgat agagtgtgca tttaataagc
     3421 acttgaggtt acgaaagtag aaaaaattag ttagatagca taaggttaaa tcctaagtgc
     3481 cttgaaaatg gataatcagc aggtaagctt cgaaaagaag aaacttcaac gactagagag
     3541 aaatctcgta cctacaagtg tggggaaatg gggagtacct aatttatggt taggtaatga
     3601 tatagtctgt gcttaataga aatattaaga agttcatagg agaactggtt aggagtaacg
     3661 accctaattg aacacactaa agatgtatta ttgaataaga ttcatataat atttaataat
     3721 tttaattata ttaagtgaat acaatatatg tttttagtat caggagaatt ttgaaaacaa
     3781 aaattaaaaa atcaagtata atcagtttag taattgcttt tagtatggta tttactgcat
     3841 ttacaccaat agtatcttat gctgacgaag tgactagcaa tgatacaata cttaatggag
     3901 aagaacaatc aaatggtcag acaccagatg tagagaaacc atcagacggt caggtaccag
     3961 gtggggagaa accatcagac agtcaagtac cagatgggga gaaaccatca gacggtcagg
     4021 taccagatgg ggagaaacca tcagacagtc aagtaccaga tggggagaaa ccatcagacg
     4081 gtcaggtacc agatggggag aaaccatcag atggtcagat accagatggg gagaaaccat
     4141 cagatggtca gatgccagat ggggagaaac cattagatga gcaaactcca gaagaagaaa
     4201 aaccattgga agaagaaata gttatagagg aaatgagttt aaaacaagat attgataaaa
     4261 ttcttgatat gactttatct caaataaata aaatagtata taacttttgg gaagatgaag
     4321 aagatgtaaa ggcagatgaa caatcagaaa ttaaccaagt ttttacttca gaagactcat
     4381 ttattagctt atggtatgac aaaaaagcaa aagtaaaaaa ttcttgttta ctaaaagaaa
     4441 tagatggttc agatagacct tatcatgatt taagatttga tgatattaca aagactttaa
     4501 cttttgacta tgtaataaag ggtcttgtag gagcaagctc aaaagacgac aaatatgtaa
     4561 tagaaggtga agatggagag caaactgcat tttgttacaa taatcattta agaccaccaa
     4621 gtagtaatgg aaagagtcca tatttaccag cagaagattt taatggtcaa gaaaaccaaa
     4681 atgaagaagc agttaaatct atactatatg caggttctga atttgatgga tttggatata
     4741 agcaacaatt taatttaggt ggagaagaaa atgaattgat gacttactca gctactcaaa
     4801 gtgcaatttg gattattctt ggacaaatgg atgaacaaga aagacttaag cagtatcaag
     4861 gttctataaa cctttgtgat aaacttatag aaagagcaaa aactgaagag gaaagagctg
     4921 agtatcagaa gaagaaagaa gtagctataa atataaaagc ttatttagaa gcattgttaa
     4981 aagcaggtcg tgaagagtta aagccaaatg atacaggaaa acctagtttg agtaatggtt
     5041 taactaagat taactttgaa aaaaatgaag atggtactta tgaaacagaa gctgtagcct
     5101 tagtaggtta tagtggtgta gtaaagttaa gactaccaga tggagtaact gcttatgatg
     5161 gagatggtaa tataataggt acaggtgaag tagaaatttc aacacaacag aaatttaaac
     5221 ttaagagtgt tgtaaaacca gatgccaaag ctaatatttc tgctgtatct tatgattata
     5281 tttttccaaa agctattcaa tattataagg cagtgcttga tttaggacaa aaagaccatt
     5341 catcttcatt accatctagt aagcaaaatt tattaagtta tacaatagag aaaaaaaatg
     5401 gtcaagaagt taactttaat ataggtctac caacagatga tgttgtaaat ccaccagtgc
     5461 caccaacaga tgatacaaat ggacataagc caaaaccatc accaccaata gatgatacag
     5521 taataaatcc accagtacca ccaatggatg atacaataat aaatccacca gtaccaccaa
     5581 cggaggatgc agtgttaaat ccaccagtgc caccaacgga ggatgcagtg ctaaatccac
     5641 cagtgccacc aacagaggat acagtgttaa atccaccagt accatcagta agtgatacag
     5701 tggaaaaaac tccagaacta tcaagagatg atacaataat aaaatcacca aaaacaggtg
     5761 atgaaactca gctaatgtca tatgtattta ttagtgtcat tgcaatttgt ggattagcgt
     5821 atcaatgtaa aataaaaaga aattaatcta gattattatt aagtttaaaa taaaaagtct
     5881 caaattgatt tataagtcat tttggggctt ttttatgtaa atataatatg caaatatagt
     5941 ttgtttaaaa atatatattt taattatatt cttaaattta tgattggata caggcattca
     6001 ttaattgttt gatgaataga aaaatgtttt gaaggtgaaa atagtattga aaaaataata
     6061 tactaagggt ataattcatt tgatttcatt tgtataaatt taattataaa ttttaaagtg
     6121 attatataaa tttaaatgct aattacttaa taaattttaa taataattat ttaataaata
     6181 attattataa tatgctagaa taaatatata taaataagtt ttgatgaggt actactataa
     6241 aggggggact ttatgttttc gataccagaa gtaaaaaaag tagaagaggt aatggataca
     6301 aaatttacaa ctatagacga agacacaaga attgaagatg caataaaaga aatgataaaa
     6361 agcaacacaa aaactctaat ggtaatagat tcatctgacc aattaaaagg cattatatcc
     6421 atgacggata tacataatct ttatgagatg cataaaaagt atgaaggtca accagtaaaa
     6481 cttataatga agaaggatgt aatatatgtc aacgaaggtt taaccttaga tgaatgtaga
     6541 gatataatga tattaaaaaa tatagggatt cttcctgttc ttagagataa taaaattatt
     6601 ggagttctaa aacaagagca cattagggac tatctatata tgcatttaga agattatgga
     6661 ttaactttga agtatattat aggtcaaatt aaagaaggaa tttgtgctat aaataacgaa
     6721 ggtgttgtta tcttgtggaa taagtttatg gaggaaaggt atgatataaa gtctgaagat
     6781 atagttggtc gaccgatgaa tgaatttttg gaaaatacta tttcagaaaa agtactaaat
     6841 agtaaagttg gaatgagtga cttgtatttt acagataaaa aagaaaatat gtatgcatta
     6901 gttcatgcaa atcctatatt ttataaagaa gaatttatcg gtgttgtatg tacagaagtt
     6961 gatgttacag aagctaagat attagctctt gaacttgaaa aagtaaatga tacattaaaa
     7021 tatctaaaga acgaagtcaa aaatttatct aaaggtagtt ttgataagat tttaggtaaa
     7081 agttataaat tggaaaaatc gaaagcgatt gcaaaacaag tggctagaac aaatagtagc
     7141 atatttattt ggggcgaaag tggtacaggt aaagaagtat ttgcaagagc tattcatgat
     7201 tatagtgaga gaaaaggtca atttatacct gttaactgta gtgccatacc aaatgagtta
     7261 tttgaaagtg agttttttgg gtatgaatca ggtgcgttta caggagcaag taaaaagggt
     7321 cgaataggta tatttgaact tgcaaaagat ggaactgtat ttttagatga aattgccgat
     7381 ttacctctta gtatgcaagc aaagttgctt agagtattac aggaaaaaga aataagaaga
     7441 gttggtggag ataccacaat aaaaataaat cctagaataa tatcagctac caataaagat
     7501 ttgagtaaaa tggtaaaggc agaaaaattt agagaagatt tatactatag attaaatgtt
     7561 gttgaaataa agattcctcc acttagagag agaaaagaag acataggatt attggttcat
     7621 agctttttag aggagatatg taaacaaaat aataagcctg ttcttacaat tagcaaagat
     7681 gtaatagata tatttcagaa ttatagatgg aaaggtaata ttagagaatt aaaaaatacg
     7741 attgaaaata ttgttgtatt gtcacaaaat tctaaaatag atgtggatga tgtaccaagt
     7801 tatatgatgg attctacaaa taatagtact gaagaagaag agtatccttt agatttaacg
     7861 aaggcaactc aaaaaattga aataaagaat ataaccaagg cacttaaaat gtctaatgga
     7921 aataaagcta aagcagctaa gatattaaat atacccagaa caactttgta ttataagata
     7981 gaccaataca aaatagatgt gtctaaaata tgacacatga ctaaattttg acaaaatgat
     8041 aaactttaat ttaaaaccta gtaaagccaa cgcttatata agtgttgtgt atatagatgt
     8101 gttaaaaaaa atgaaaaagt gttgattaat tgacactttt tttgtttgag aaaatgttta
     8161 caaatacaaa aaataacata ttttaaataa atatttatat aatatttggc taaaaatagc
     8221 gatagatgaa gaatctatta tagaaaaaat ctttttgtta ataagttggc ataggaattg
     8281 cttataatat aaagtgactt taagtgagaa aaaaataata ttaatcataa caaattatgt
     8341 aggttttaat ttacttaatt tctcagttaa ggggttacct gttgaggaat tgagatttaa
     8401 aataataaat aagaaaataa tgtaaggagg tattttatac tatgtcaata actttagaaa
     8461 cagctcaagc ccatgcaaat gacccagcag tttgttgttg tagatttgaa gcgggaacaa
     8521 ttatagcgcc agaaaactta gaagatccag caatatttgc agacttagag gattctggat
     8581 tattaacaat accagaaaat ggattaacta taggtcaagt actaggagct aagttaaaag
     8641 aaactttaga tgcactttct ccaatgacta cagataacgt agaaggatac aaagcaggag
     8701 aggctaaaga agaagtagta gaagaaacag tagaagaagc agctccagta tcagaagcag
     8761 cagtagttcc agtaagcaca ggagttgcag gtgaaacagt taaaatacac ataggtgaag
     8821 gtaagaacat aagcttagag atacctttat cagtagctgg tcaagcagga gttgctgctc
     8881 cagtagcaaa cgttgctgct ccagtggcaa gtgcagcagc agaagtagct ccaaaagttg
     8941 aagaaaagaa acttttaaga agcttaacta aaaaacactt taaaatagat aaagttgaat
     9001 ttgctgatga aactaaaata gaaggaacta ctttatacat cagaaacgca gaagaaatat
     9061 gtaaagaagc taatgaaact caagagttag ttgtagatat gaagttagaa ataataactc
     9121 ctgataaata tgaaacttac agtgaagctg tattagatat acaaccaatc gctactaaag
     9181 aagaaggcga attaggttca ggtataacta gagttataga tggagctgta atggtattaa
     9241 ctggtacaga tgaagatgga gttcaaatag gtgaatttgg ttcttcagaa ggtgagttaa
     9301 atactactat aatgtggggt agaccaggtg ctgctgacaa aggtgaaata ttcatcaaag
     9361 gtcaagtaac aataaaagca ggaactaaca tggaaagacc aggaccttta gctgctcacc
     9421 gtgcatttga ctatgtaact caagaaataa gagaagcatt aaagaaagtt gacaactctt
     9481 tagtagttga tgaagaagta atagagcaat acagaagaga aggtaaaaag aaagttgttg
     9541 ttataaaaga aataatggga caaggtgcaa tgcatgataa cctaatatta ccagttgagc
     9601 cagttggtac attaggagct caaccaaacg ttgacttagg aaacatgcca gttgtattat
     9661 ctccacttga agtattagat ggtggtatcc atgcattaac ttgtatagga cctgcatcaa
     9721 aagaaatgtc aagacattac tggagagagc cattagtaat aagagctatg gaagacgaag
     9781 aaatagattt agtaggtgtt gtatttgttg gttctccaca agtaaatgct gagaaattct
     9841 atgtatctaa gagattaggt atgttagttg aagctatgga agttgatgga gctgtagtaa
     9901 ctactgaagg tttcggaaac aaccatatag atttcgcatc tcacatagag caaataggta
     9961 tgagaggtat accagtagtt ggtgtaagtt tctcagctgt tcaaggtgct ctagttgttg
    10021 gtaataaata catgactcac atggtagaca acaataagtc taagcaaggt atagagaatg
    10081 aaatattatc taacaacact ttagctccag aagatgctgt tagaataatg gctatgctta
    10141 aaaatgctat agaaggtgta gaagttaaag ctcctgaaag aaaatggaat ccaaatgtta
    10201 aattaaataa catagaagct atagaaaaag ttacaggaga aaaaatagta ttagaagaga
    10261 atgagcaatc tctaccaatg agtaagaaga gaagagaaat atacgaaaaa gacgaaaact
    10321 aaaatttaga tactaactaa gatttagaca ataacataaa tttagatcca agtttaaaac
    10381 agtatagaga ggtagaatta taatggatag gttaaaatat atctcatcag agacttttta
    10441 tgaaggtatt atagttgaca tcaaaggcgg tggagtgact atagacctaa aaggtagact
    10501 tggacaattt aaaataccta atagaatgct tataactgac tacgaactta agataggtca
    10561 agaagttgga ttcatgctaa gctatcctga ggtattaagt ccagagccta atcaagagta
    10621 tgtagaaaat attaggagag aaaaagaaaa acaagcagaa atgcaatcaa aacaagaaga
    10681 aaaataataa ataatcgttt gaaaatagaa gggagaggaa atatatgagc cttacaacag
    10741 tacaaggact tcaatctgaa atattcgttc caataacacc tcctccagtt tggactcctg
    10801 taactaaaga attaaaagat atgactatag ctttagctac agcagctggt gttcatttaa
    10861 aatctgacaa gagatttaat ctagcaggag atactacatt tagagctata cctaatacag
    10921 ctacagttga tgaaatgatg gtatcacatg gtggatatga caatggagat gtaaacaaag
    10981 atataaactg tatgttccct atagatagat tacatgaatt agctgcagaa ggatttatca
    11041 aaggagtagc tcctatgcac tatgcattca tgggtggtgg aggaaaccaa catgtcttca
    11101 ctgaagaaac tggtcctgct atcgctgcta aacttaagga agagggagta gacggtgtag
    11161 ttatgacagc tggctgaggt acttgccata gaactgccgt gatcgtgcag agagcaatag
    11221 aggaagctgg aatacctaca ataataatag cagctcttcc tccagtagtt agacaaaacg
    11281 gaactcctag agcagttgct ccattagttc caatgggtgc taatgctggt gaaccacata
    11341 atatagaaat gcaaacacat atattaagag atacattaga gcaattagtt gcaataccat
    11401 ctgctggtaa gatagttcca ttaccatacg aatataaagc tcacgtttaa aatgtaattt
    11461 atatttgaaa atatagaacc cttctcctac acggttagga gaggggtttt aagaacaaag
    11521 gtggatatta tggaagaaaa aataatgaga agacttgtta taaaaccgtt tcatatgaat
    11581 gaagttaatt tcggttcaaa aacttctata aagaaagatg tacttacaat agatttatca
    11641 agtatagatg aaataaaaga aagagaagag ttaataacag acataaaagt agacattata
    11701 aaacctggag attatgacag agaaattaat actattatgg acattatacc aatatcaaca
    11761 aaagttttag gcagactggg agagggtatt acacatactt taacgggtgt atatgtaatg
    11821 ttaactggtg cagatgaaga tggaaatcaa atgcatgaat ttggttcttc agaaggaatt
    11881 ttgaaagacc aaatggtatt tgggagatat ggaactccat cagttgaaga ttatataatc
    11941 catgtagatg taactttaaa gggaggttta ccttttgaaa gaacacttcc acttgcagca
    12001 ttcagagcat gtgatgattt tatacaagaa ataagagcgt ctttgaaaat ggaagacgga
    12061 agaaatgcta ctcaagttcg tgaatatttt gacaaaattc gaccaaatgc aaaaaaggtt
    12121 gtcatagtaa aacaaatagc aggtcaaggt gctatgtatg acaatcagtt attctcaaag
    12181 gaacctagtg gatttgaagg aggaacatca attatagata tgggaaatgt acctatgatt
    12241 atatctccta atgaatacag agatggtgcc cttagagcta tgacttaaga gaggtggata
    12301 ttatgggaat aggaccttca acaaaagaaa catctcttca tcactttaga gacccacttt
    12361 tagacatagt tgaaagtgat aaggatgtag atttacttgg agtaatagta gtaggaactc
    12421 cagatggaaa tgaaaataaa acctttgtgg gacaaagaac ggccgcttgg ctggaagcca
    12481 tgagagtaga tggagctata gtttcatcag atggttgggg aaactcacat gttgactacg
    12541 ctaacacatt tgaggaaata ggaaaaagag atatacctgt agttggagtt acatttaatg
    12601 ggactcaagc taagtttgtt gtaagcaatc aatatatgga taccatagtt gatatgaaca
    12661 aatcaaaaga aggtattgag acagaagtag ttggagaaaa caacacaaat gaaatagatg
    12721 ctaaaaaagc tttggcattt ttaaaactta aaatgagaaa acatggataa aaaatttaag
    12781 taagggaatg agttttgtgg gaatttctac atcaatgaaa aacacatcac ttcatatatt
    12841 tagagacccg ttactagatt tgatagataa tgattctgac atagacttgt taggagtggt
    12901 tgtagttggg acatcagagc acaatgaatg gaaaacattt ttagcaacta gacttggaag
    12961 atggattgaa tcattgcgac ctgatggagt gattataact ttagatatgg caggaaacca
    13021 gcatattgat tttacaaatg ctatagcaga gtttgtaaaa tcagatatac ctacagtagg
    13081 tcttactatt atgggagcag atggattagt aattaccaat ccatatttgg ataaagctac
    13141 cataattgat tataaaaaga caacaggcta catagaaaca gaagtagttg gagacaatca
    13201 catggatgaa gttgatgtta aaaaagcatt ggctttccta aaattaaaaa tgcgtaaaga
    13261 cgcaaaataa acctaagggg gaatttgata tgaaatttag cagaagtata caagctatag
    13321 actctcatac agcaggtgaa gcgactagaa tagttgtagg tggaatacct aacataaaag
    13381 gaaattctat gcctgagaaa aaagaatatt tagaggaaaa tctagattat ttaagaactg
    13441 ctataatgtt agagccaaga ggacataatg atatgtttgg ttctgtaatg actcaacctt
    13501 gttgcccaga tgctgacttt ggaataatct tcatggatgg tggcggatac cttaacatgt
    13561 gtggccatgg tacaatagga gctatgacag cagctataga aacaggtgta gttccagcag
    13621 tagagcctgt aactcatgta gttatggaag ctccagcagg aataataaga ggagacgtta
    13681 cagttgtaga tggaaaagct aaagaagtat cattcttaaa cgtaccagct ttcttatata
    13741 aagaaggtgt tgaagttgac ttaccaggtg ttggaactgt taaatttgac atatcatttg
    13801 gtggaagctt ctttgctata atacatgcaa gtcaattagg tttaaaaatc gaacctcaaa
    13861 atgctggtaa attaactgaa ctagctatga aacttagaga tataataaat gagaaaatag
    13921 aaatacaaca tccaacttta gctcatataa aaactgtaga cttagttgaa atatatgatg
    13981 agccaactca tccagaagct acttacaaaa atgtagttat atttggtcaa ggtcaagttg
    14041 acagatctcc atgtggaact ggaacaagtg ctaaattagc tactttacat gctaaaggtg
    14101 aattaaaagt aggagaaaaa ttcgtatatg aaagtatatt aggaacttta ttcaaaggtg
    14161 aaatagttga agaaactaaa gttgcagatt tcaatgctgt agtacctaaa ataactggtt
    14221 ctgcttatat aactggattt aatcattttg taatagatga agaagaccca cttaaacatg
    14281 gatttattct taaataagta ataaataaat taggaaatta aaatagaata gtagccactt
    14341 gtcggttgct attctatagc ttttaggcta taagattcag aatatatgaa aaaataaaaa
    14401 aataggagga tggattatgg tagcaatctt aaaggcatta tatgtagtat tcgcagcaat
    14461 ctttgcagtt gtgtttggta aagacgtagc taaagcaaac aaagagggga aactagaaga
    14521 acaaagtact ccaaaagttg ctgtaacagg atttataaca gacttctttg atacattagg
    14581 aatagggtca tttgctccaa caatagcaat gtcaaaggct ttaaagttaa atataccaga
    14641 taaaaaaatg cctggtacat taaacgtagc acatactatt ccagttgtta cagaagcatt
    14701 catatttaca tcaattatcc cagtagatgg tgttacttta gtatctatgg ttgtggcagc
    14761 agcagttggt tcatatatag gtgcaggaat aatagctaag atggacgaga gaaaaataca
    14821 acttgttatg ggtgtaactt tagcattaac agcaatatta atgttattag gtcatccttg
    14881 gattaacgta ttaccaggtg gaggaaatga attaggtctt actggtgcta aattagtaat
    14941 aggtgtaata ggtaacttta tactaggagc tttaatgaca gctggtatag gtctatatgc
    15001 tccaggtatg gcaatggttt atttcttagg aatgtctgca aaagttgcat tcccaatagt
    15061 tatgggttct tgtgcattat taatgccagt tgcaagtatg aaattcataa aagaagatgc
    15121 atatactaaa aaagcatcag ttataatagc tatatgtggt ttaataggag ttttcgtagc
    15181 tgcatatata gtaaaaactc taccaatgga tatattaaaa gcattagtaa tagtagttat
    15241 agcttatact tctgctacaa tgataatggc tgcaaataaa aacaaaaaga ctgcttagtt
    15301 aaagactact tagtgtttat taaatattac agctaataat aaagattata aactgcttta
    15361 aaatattgaa ttaatatttt agggcagttt tttatttact aaattaatat aaagcaatta
    15421 acatgccaat tatgtaatat atatagtgat ttagtagatt taatctaaaa aacgttgtat
    15481 ttttaaagaa taaagcataa tttaaagcta aatatttaaa ttttataatc aataagttca
    15541 ataaatgttc atatattaat acagattaat aaaaatataa cctgtaataa gtctaatata
    15601 acttataatt aagaaaaata agtttataat gactcaatat tcatctgaat tatttcaata
    15661 aatttaattc gttgaagttg cttatataga gtgaataaat ttaagttata tatgacttat
    15721 taattatata ataggatttt tataattaat acaaaactga aataggtagt ttaattagta
    15781 tatgtggtac aaaaagataa taattttata attataaaag tgaaatatta aatgaaattt
    15841 ataactaaat aaaaactttt aaacagcaat caaagatttg taataaaata catcccttat
    15901 aaatatattt attatataag ctgataccag aaatataaga attaacaatt gtggagggat
    15961 aaaatatgat agctgttaaa gatgagaata acgtagtaaa cttaagaggg gagttagaca
    16021 ataagctaga atttagccat gagatatttg gagaaaaatt ttataatatg aaaataaaaa
    16081 taaacagact aagcgattct tttgatatac ttccaatgac tgtatcagaa agacttctcc
    16141 aagatattga ccttgataaa caaaatttag tcaatgtcgt aggccaatta agatcatata
    16201 acaaaacatt aaataataaa aatagattag tgcttacagt gtttgtcaga gaaataaaaa
    16261 gtatagacga ggaaaataaa gaccctaata gtatattttt agatggatat gtatgtaagg
    16321 aacctgtgta taggaagaca cctttaggaa gggaaataac cgatttatta gttgctataa
    16381 acagacctta taataaatct gattatatac catcaatagt ttggggtaga aatgctaaat
    16441 ttgcaaagaa tctaaaggtt ggtgatagaa tacagctttg gggtagggtt caaagtagag
    16501 aatatgagaa aaaaatagat gaaaataatg tggtcaaaaa aatggcatat gaagtatcca
    16561 tatcgaaaat aaaaaaattg gatgaaaata acaatgaata aacataaaaa atagactgat
    16621 ttttatgaat aaaatcctaa taaatttggt ataatatcat attaaaatgt ataaagattg
    16681 tttaaaaaaa tactaatgaa atatctcttg aatagttgct cgccatttat tataatgtaa
    16741 tctgtacata atttataatt gaggaaatga agaggtgaag cattgtgaaa agcatattat
    16801 gtcagataaa taaacagcca gttaaagcta aatatacatc tgatgaatta gaatgttttt
    16861 tggatacaat tgagtataat gtaggtgata aaatattgct tgttggtcat ataggaaacc
    16921 taggcaagag attgagaggt cttggaactt ctgtaactat actggagaat agtaattatg
    16981 aagatgtctg ttattctcta gtgtataatg aaaattgcaa tgtagttaaa ggtaagttag
    17041 agtttctacc atttgatgat aatcagttca acaagataat aatagttgac caatttaatg
    17101 ttattacaga ttgtgaaaaa gcttcatctg aaatacaaag agtgttaaag ggcaatggag
    17161 aggttatatt agaggattta aatttaaaga atttaaaagt taagttaaac tatcttaaac
    17221 ataaattttg tggttctgtt gcaaagattc actatccaca tgaagtattt aatatatttt
    17281 caagattaaa ctttgatggt atgattaaag agtcaaataa attaaaatat atttatgtgg
    17341 gaaaaagaag ataaatgagt taaaatataa atatggatat aatataaaag actaggttaa
    17401 gcctagtctt ttgttataag gagatgttta gatggaaata aattatgagt taataataat
    17461 tgttatgctc tttgtaatcc tgatgtcaat ccaatttact ttaaataaga tatacttgat
    17521 tcttaaagaa ataagacaaa tacttatgct aaagaaaaat aaggaatgat gattatgata
    17581 ggtttagaat atataaaatc tgatattcaa aatatagctg aagctatagt atcagtactc
    17641 aatatagatg taactatagt aaataaggag ttatttagga ttgcaggtac aggagtatat
    17701 atagacaaaa taggtgagaa agtagataaa tatacggctt ttaaaaaatc acttacagaa
    17761 cagattacaa tactaattga tgacccaaag agtagtgata tatgtaggga atgctataaa
    17821 agtggggctt gtgtagagtt tgcagaggtg tgttgtccta ttatatgtga tggcgattct
    17881 catggtgtta taggccttat agcctttaca aaagaacaag cacaaattat agaaaataat
    17941 aaaagtggtc ttataaactt tttaggcaaa atggcagacc taatatctaa taaattaaaa
    18001 gcacaaatta aaacttatga gcttgaatta gaaaagaaaa aattagagac actattaaac
    18061 aatatggaca aagctgttgt gtctattgat gtggacggaa aaattgataa atataattca
    18121 aaatttaaag agcttttcaa tataaaagat agtataactg gcaaaaacat atttgtcgaa
    18181 ttagatttta taaagaaacc ttctataaat aactttaaga aggacaaatc ctgtagtttt
    18241 ttttataaaa gatacggata tgattcgaag ggaatataca acatcaataa aatagtatta
    18301 aaaaatgagt taaagggata tgttattgat tttatagata agagagatgc tataaaaaac
    18361 tacaataaga tgaataaaga ttatagaata aaattggaaa acataattgg agaaagtgta
    18421 gctataagaa attctaaaaa ggaagcccta atagcttcta aatcaacatc aacagtactt
    18481 attactgggg aaagtggaac gggtaaggag ctatttgcaa gagccataca caaccatagc
    18541 aatagagtag ataatccttt tatagctgta aactgtgctg ctataccaga caatttatta
    18601 gagagtgaat tatttggata tgaagaggga gcttttactg gagctaaaaa aggtggaaag
    18661 cttggaatgt ttgaagtagc tcataaggga agtatatttt tagatgaaat aggagatatg
    18721 agtttgcatt tacagtcaaa gttattgaga gtattacaag aaaaggaact taataaaatt
    18781 ggaagtaaat caaatgtttc aatagatgta agaattattg ctgctacaaa taaagattta
    18841 gaaaaaatgg ttcaaaatgg cacttttaga gaagatttat actatagatt aaatgttata
    18901 cctataaaat taccaagttt aagagagaga aaagaagata tcccacttat aatcaagcat
    18961 atggttaaag agtattcaaa aaaattagat aaaaatgtta ttgacataga tagcagggtt
    19021 gttgatgcta tgatggaata taagtggcca gggaatataa gagagttgca gaatattatt
    19081 gaatatagtg tgaatatgtc ttcttcttca ataatcacaa tgaatgtaat tcctcaaaaa
    19141 attaagaata cctctgtaga agagttagaa gttaaaagcg ataggataat tccacttgat
    19201 gaactagaga agattgagat aataaaagct ttaaataagt acaaagatta taaaaaagat
    19261 aaagaattaa cagctaaagc acttggaata tcaagagcta cattatatag aaaaattgaa
    19321 aaatacaata taatttcata gtaatataat aaaaagctat ataaaattta aatttttcta
    19381 aattcataac tttaaagaaa gctttaaact tatactataa gtgttttgca gaatcttttt
    19441 ttatgaatta aaaaatggtt tttataagtt aaaaaataat acatttgagc cgtctcaaaa
    19501 tgatatagaa tctcattttg agactttttt ttgtcttttt gcgaaattgt catatgtttc
    19561 tccattttaa agaatataaa tactctatgt aaataaaaaa aatacataaa tagaagcagt
    19621 cataccaatg gttatagaat ataagactaa aaatataaaa agatataaaa aatatggcat
    19681 gtattttgct ataaatatag ataagaaatt atttaattaa ttatttgagt actaaatcta
    19741 aacttaattg ggaggtttat atataatgag agatataaga aaagaattag tggaaatgtt
    19801 aaaggcagaa gttaaaccag cagtagggtg tactgaacca gtagccttag ctctagcatg
    19861 tgcaaaggca aaggaattat taggagaaga aatagttgaa aatcgtatgt tagttagtcc
    19921 tagtatttat aaaaatggta tgtgcgtagg tataccaggt actgaaagat taggattaaa
    19981 aattgcagca gctctaggta tagtaggtgg acattctgaa aatggattaa gtgtgttaga
    20041 aactctaaca aaagaggaag ttaaaatagc tgaggattat atggataata ctccactatc
    20101 aataacacct gctgatacaa gagagaaagt atttatagaa gttgtgttaa aaggaaaaaa
    20161 tcatattgct aaagtgagaa taagaactaa acatgataat tttacgttct tagaaaaaga
    20221 tggagaagta ttattagaca atgaaccaaa agtatcagct tcaaatgatg cggcagaaaa
    20281 agctgaaagt ttaatggata cagtaactat acaagaatta ataaaaaatg tagaaggaat
    20341 tgattttaaa gatatagaat ttttactaga tggagtaaaa atgaatgaag agatggctga
    20401 atatggatta aaacaaaaaa ctggtattgg tgtaggatat ggaattaaaa agtctataga
    20461 agaaggtctt ttaggtaatg atgttataaa ttatgcaatg atgttaactg ctggagcttc
    20521 agatgcaaga atggcagggg taaaaatgcc tgttatgagt tcaaatggaa gtggaaatca
    20581 tggattaaca gcaattcttc caatcgttgc ttataataaa aaattcccac aatctgatga
    20641 aagactagca aaagctttag ctatatctca tctagtaact ggttatatta agaactatac
    20701 aggaagatta tctgctgtat gtggatgtgg tgttgctgct tctactggag caactgctgg
    20761 tatctcttgg cttatgaatg gtactgaaaa gcaaatagaa ggagctatag aaaatatgat
    20821 agctgactta agtggaatga tatgtgacgg tgcgaaagct ggttgtgcat taaaattatc
    20881 ttctgcagca tctgcagcta tacaaagtgc tataatagct aagcaagatt gttttgtacc
    20941 accactaaat ggtatagttg gttcaagtgt tgagcaaagt atacaaaact taggtagagt
    21001 aagtgacaaa ggtatgtcta taacagatga gataatctta aatgtaatga atgatatgaa
    21061 taaagttgac tagtacttta taatatgttt tgtattatga ggtataacaa aaatagcata
    21121 aataaattta attagtaccc tctaataaaa tatgattatg tataaattta tgtataatag
    21181 aaaaaacttg ttagtgcaag ttttttcttt ttttgtctaa aatatatagt atgtaattta
    21241 gtgtggatat atgaactact taaaatataa gatacattgt taataattgt tgaatgaaca
    21301 taaaaactca ataatattga ctaatattga ataagagtat tgaaaaagtg agcaatctct
    21361 tataaaatta agaaggatta ttacaattgg aggtcttttt atggatatag aaacaaaaaa
    21421 atgggaagta ttgtattcag aagaaaaaat taaagataaa ttaagggaat taggagcaat
    21481 tatagaaaaa gattataaag ataaaaatct tatggtagtt tctctattaa aaggtagctt
    21541 tatattttgt gcagacttag ttagaaatat aaatttacca cttagagtta attttatgac
    21601 tacatctagt tatggaaaca atgaagaaag tactggaagg gttaaagtag tttcagatgt
    21661 gactactgat atagcaggat atgatgtttt ggttgtagac gatattactg attctgcact
    21721 tactatggat tttgtgttaa aacatctaaa agctaaaaat cctgctagtt taaaatgctg
    21781 tgttcttttg gataaaccaa gcagaagaaa agtcgattta gtgccagatt attgtggatt
    21841 tgaaatagaa gataagtttg tggttggata tgggtttgat tttggtgatt attatagaaa
    21901 tgttccttat atattcaatg ttactgatga agatagataa gcaattatat aattttgtgg
    21961 atgccttaat atgattttta ttaatcgtgt tgaggttttt ttatttaaaa agtaattaaa
    22021 gtagtattaa attaatttta tgcaatttaa ttatctattt tttataacat aataaaaaaa
    22081 ttttaaataa ttgatgggaa atgagttttt taattgtatt aataattgta aaagagtact
    22141 aataattgta aaagatttta caaaaaagta atgaaattca agaatcaaag catatatgtt
    22201 gatataagca aatagataca ttatcacata agaaattgtc gattcatata attatataga
    22261 gtaatactca atatttatag agtaatactc taactttaaa aaaggaggat taattatatg
    22321 agtgatattt caggtccaag tttatatcaa gatgtaggtc caacagggcc aacaggtgct
    22381 actggtccaa caggaccgac ggggcctaga ggtgcaactg gagcgaccgg agcaaatgga
    22441 ataacaggtc caacaggaaa tacgggagca accggagcga atggaataac gggtccaaca
    22501 ggaaatatgg gagcgactgg accaaatgga acaacaggtt ctacaggacc aacaggaaat
    22561 acaggagcga ctggagcgaa tggaataaca ggtccgacag ggaatacagg agcaaccgga
    22621 gcaaatggaa taacaggacc aacaggaaac aaaggagcaa ctggagcaaa tggaataaca
    22681 ggctctacag gaccaacagg aaatacagga gcgactggag caaatggaat aacaggacca
    22741 acagggaata caggagcaac tggagcaaca ggtccaaccg gactaactgg agcaaccgga
    22801 gcaaccggag caaatggaat aacaggtcca acaggaaata caggagcaac cggagcaaat
    22861 ggagtaacag gagcgacagg accaacagga aatacaggag caacaggtcc aaccggaagt
    22921 ataggagcga ctggagcaac cggaacaact ggggcaacag gcccaatagg agcaacaggt
    22981 ccaaccggag cagatggaga ggtaggtcca acaggagcag taggagcaac aggtccagat
    23041 ggtttggtag gtccaacagg cccaacaggc ccaactggag caaccggagc aaatggtttg
    23101 gtaggtccaa caggagcagt aggagcaacc ggagcaaatg gtttggtagg tccaacagga
    23161 gcgacaggag caacaggagt agctggggca ataggtccaa caggagcagt aggagcaaca
    23221 ggcccaacag gagcagatgg agcagtaggt ccaacaggag cgacaggagc aacaggggca
    23281 aatggagcaa caggcccaac gggagcagta ggagcaaccg gagcgaatgg agtagcaggt
    23341 ccaataggtc caaccggtcc aaccggagca aatggagtag caggagcaac aggagcgaca
    23401 ggagcaacag gggcaaatgg agcaacaggc ccaacaggag cagtaggagc aacgggagca
    23461 aatggagtag caggagcgat aggaccaaca ggcccaactg gagcaaatgg agcgacagga
    23521 gcaacagggg cgaccggagc aacaggagca aatggagcaa caggtccaac cggagcgaca
    23581 ggagcaacag gagtgttagc aacaaacaat gcacaattta cagtgtcctc ttcaagttta
    23641 gtgaataata cattagtgac atttaattca tcatttataa atggaactaa tataactttt
    23701 ccaacaagta gtactataaa tcttgcagtt ggagggatat acaatgtatc tttcggtata
    23761 cgtgccacac tttcacttgc aggatttatg tcaattacta ctaactttaa tggagtagct
    23821 caaaataact ttattgcaaa agcagtaaat acacttactt catcagatgt aagtgtaagt
    23881 ttaagctttt tagttgatgc tagagcagca gctgttactt taagttttac atttggttca
    23941 ggcacgacag gtacttctgc agctggatat gtatcagttt atagaataca atagtttata
    24001 tgatgaaaag attataaaat aataaacaat atgatttcat ataccaataa atagaataat
    24061 atttaatagg tttatatgat gaaaagacta taaaataata aacaatatga tttcatatac
    24121 caataaatag gataatattt aataggttta tatgatgaaa agactataaa ataataaaca
    24181 atatgatttc atataccaat aaataggata atatttaata ggtttatatg atgaaaagac
    24241 tataaaataa taaacaatat gattccatat accaataaat aggataatat ttaataggtt
    24301 tatatgatga agagattata aaataataaa caatataatt ccatatacca ataaataaga
    24361 taatctttaa gaaaaatgat tatatttaaa aggtaaacat aaaatttata aacttttaga
    24421 atagaaatct caatcaatta gatattcaaa ttgatgaata tgaataccta aatagattga
    24481 gatttattat gtacaaaact tatcctaata tatctgacat tttataaagc ccagattgct
    24541 taccaattaa aaatttagct gcaactatag aaccgtttgc aaatacttca tcagattgag
    24601 aatgatggct taatgtaaga acttcattat cacctgcaaa tacaacatca tggtcgctga
    24661 ttatattgcc acctcttata gagtgtattc caatttcatt tgattttctt ttacaatctc
    24721 taccatatct accatgtgta ttttcacttt caggcaagac ttctctaatt gcattagcta
    24781 tcatagtagc agttccacta ggggcatctt cttttctatt gtgatgtttt tctatgattt
    24841 caatatcaaa accttctaac atctttgaag catccttaac taattttatt aatatattca
    24901 ctccaagaga tgtatttgat gataataata taggaatgtt ttttgatgct tcttctattt
    24961 tatttaagtc attttcatca tatccagtag tagctaatac tacaggtgta ttatttttta
    25021 gagcatatga caatattggc tctaaagtct catgatgtga aaagtctata attacatcag
    25081 ttttttcagc tacatcagtc attgttttgt aaagctttgt atcactatga tgaccttttt
    25141 catcagcaga cctagctaca ccacaaacca actctaattc attatctttt tttacacatt
    25201 ctgctaaaac tcttcccatt gttccattat agccactaat tataactttc aacataaatt
    25261 cctcctatga aattaataaa aatttgttat atttctataa gtttatttta tcactaatat
    25321 aatttatata aaataatatt agtgataata agcttacaat ttatcttaca ttaaaaaatt
    25381 taagtggatt tttcaaaata tagataaatt tctttttcat tgtataaatg ataaaaatat
    25441 atttgacttc ataccaaaaa gagtgcccca tgaactaaaa agttaatttt gaaataccat
    25501 attcatatat catatgattt atttggaaat caatattaat acattaaaat aactaaatac
    25561 atgtatgcat aaagatgtat aaaaaatata taaacatata gtattaattt taaattcaac
    25621 aatttaattg ttaaaagtgt tattattaaa ttagaaatat cttaacttaa tatactgaga
    25681 cgggagtgat agatatgtta aaagaagaaa aaacttatca agagaaagac gcaaaagtat
    25741 tcaatggagt ggcagctttg ataatgattt tattattttt agtagtttca ataggtatca
    25801 tggtttttgg gatagtaaaa cttgatagtg gatttactgg aataggttca cttatgttaa
    25861 ttttgggact tgtatttata gtagtagatt ttatcctatt ttttgggcta aaaatgatta
    25921 atcctaaaga agctatagtt ctagttttgt ttggtaacta ttatggaact ataaaaaaag
    25981 aagggtacta ttgggtgaat ccattttgtt cagcaataaa cccagcagta tcaagaatta
    26041 atgtatcgaa atcttcatct gatgaaaagg tagatatatc aactaattca agtggcaaaa
    26101 aagtatcttt aaaaacaatg actcttaaca atgaaaagca gaaagtcaat gatttattgg
    26161 gaaatccaat tataatagga gtggttgtaa tttggaaggt tatagatgca acaaaggcag
    26221 tatttaatgt ggataattat aatacattct tatctattca gtgtgattct actattcgta
    26281 atgtttcaag actttatcca tatgatgtat ctgaagatgg agatgaaaaa tctcttcgtg
    26341 gtagtagtca ggaaatagca gatagattaa aagatgaatt acaatcaagg gttgatattg
    26401 caggaattga agtgtgtgaa gtgagaatta ctcatctttc ttatgcacca gagattgcag
    26461 cagcaatgtt acaacgtcaa caagcagagg ctataatagc agcacgtaaa aagattgttg
    26521 agggggctgt aagtatggta gagatggcat tgactaattt aagtgaaaat aatgttgtga
    26581 ctttggacga agaacgtaaa gcagctatgg taagtaatct gttagttgtg ctttgtggca
    26641 ataaagatgc acaaccagta gtaaacagca gttctattta ttaaattata aaaagtttaa
    26701 tagtttattt tattgatata tagaaaattt agtagttcat tttattaaat tatagaaaga
    26761 ttaatggttt gctttattga attataaaaa gtttaataat ttattctatt gatatataga
    26821 aaatttagta gttcatttta ttaaattata gaaagattaa tggttttcac tttattaaat
    26881 tataaaaagt ttaatagttt attacattga tatattgaaa aattgaagga aataaaaata
    26941 cttgtcagca agaaataaaa atgatataag aatgaataag agaattgtaa aaaaataaaa
    27001 tgattataaa taaaagataa aaatattgta aaaaagaggg tgcctctggt gaattaaatt
    27061 cacacagaga caccttttta attaattttt taaattagta tattattgtt taattaatag
    27121 catagtacta tttaattagt ctaaatttct aaggtcttcc ataagcttag ttttgtcagc
    27181 cgttttttca tctttcattt ttataacttt agcaggagaa ccagcaacaa cggcaccagc
    27241 ttctacatca gtagttacaa cagcaccagc tgcaacaaca gcacctttac cgattctaac
    27301 accttcaaga ataactgcat tagctcctat taaaacatca tcttcaacta taacaggagt
    27361 agcagaaggt ggctctaaaa caccagcaac aacagcacca gcacctaagt gaacattttt
    27421 accaagagtt cctctagctc caataacagc attcatatct accatagaac cttcaccaat
    27481 aacagctcct atatttataa cagctcccat cattacaaca gcattttttt ctatagtaac
    27541 catgtctctt attattgcac ctggctcaat tcttgcatgt tcacttaaag tattttttag
    27601 aggaatagca gaatttcttc tatcatattc tacatgagta tctgttatat catttccatg
    27661 tttatctaat acttccatta tatcctcata gtcacctaca aatatataag aaccattact
    27721 tccaaatacc ttaaatttat cagttgattg gaagtgtaaa gagttagtat ttacatatac
    27781 cttaacaggg gttttctttt taacttcttt tatatacctt gctatttcat aagcatcgtt
    27841 taaatttacc atttgttaat tctccttgta tttcattata tttcttttat tatatactta
    27901 tttgtcaatt ttagtatata cttacttctt attatttata ttttatttag tttttattgt
    27961 atagacatag tattaaataa atttattata tatgtttgat ttgtattgcg tattattata
    28021 tctatattgc atatgtttga tttgtattgc gtattattat atctatattg catatgttta
    28081 atttatattg tgtattatta tatttatact attatcccaa catgctgtcc atattataaa
    28141 atcctggttt ttgcttaact aagtatttgg cagcagctat agaaccactt gcaaagatac
    28201 cacgagattg agcttgatgg cttattgtaa gaacctcatt gtctccagca aatagagcat
    28261 catgttcgcc aacaatattt ccacctctta tagcatgaat accaacttca tttggttgtc
    28321 tttttgcact acgaccatat ctaccataat tattttcaac gtttggcaaa acctctttta
    28381 tagcgttagc aatcataaca gctgtgccac ttggggcatc tacttttctg ttgtgatgtt
    28441 tttctattat ttctatatca aatccttcta gtaatttagc tgcatcttta accaatttta
    28501 ctaatacatt tacaccaaga gacatattat atgattggaa tacaggaata actttagccg
    28561 cttcacgtat tttgttcatt tcatcatcat tatatccagt agtagcaatt actaaaggtg
    28621 ttttagtctt taaaacgtaa gataagatat tatctaaatt agagtgatga gaaaaatcta
    28681 taactacatc agcttcctca actatagtac tcatatcttc ataaatttta aggtcgcctt
    28741 catagctatc cagacctcta gctgcaacac aaaccaattc taattcatta tcttctctta
    28801 tagtatcggc taaaatctta cccatttttc catcataacc agttgctata acttttaaca
    28861 taatttcctc ctatgattaa gggctaactt ttgaccaaac ttacttttta ccatgcatta
    28921 atctttacta tgcattaact tttagtccaa agttaactaa ttctttcttt aatacttcta
    28981 aatttgctgg gtccatttct gctagaggta atcttaaatc tccaacattg aatcccaata
    29041 aattcattgc agtcttaact ggtataggat ttacttctat aaataaagct gcgattagag
    29101 catccatacc aagttgtaat tttctagaac cttcaatatc gccttcaaag aatttagcta
    29161 ccaaatcgtg agtttcttga gggcaaacat tagcaagtac tgatataact ccttgacctc
    29221 ctaaagataa taaaggaagt atagtatcat cattaccaga gtagatagca aagtcatctg
    29281 gaactaaact agctatttct gcaacctgtc ctaggtctcc actagcttct tttacagcaa
    29341 ctatgttatc aattttagca agttcaacta taaggctagg acttatattt acacaagttc
    29401 ttccagggac attgtaaagt ataattggaa gtttaacact gttagctatt gtttcaaaat
    29461 gacgcttaag tccagcttta ttagctttat tgtaataagg tgttattatt aatagagcat
    29521 ctactcctaa tttttcagct tcttgactta agttaactga gtgcatagta tcattagaac
    29581 cacttccagc tataacagga actcttttat taactttttc aacaacatac ttaatagtag
    29641 caagttgttc ttcatcagtc atagtattag cttcaccagt agtaccacaa gatactatag
    29701 catcagtacc attagctatt tggtattcaa ctaattcacc taatttgtca aaatcaactt
    29761 tgttatcctt tgtaaatggt gtaactagag caacagcaga acctttaaat atcatataaa
    29821 atcaatcccc tcttttttat ttttgtgttt tatattacag ttacttctaa tgcttgtttt
    29881 tgagttacta ttaatatttt acaaaattat atatttatac aagtttgtat attatactaa
    29941 atcgtatgat aaagccattt cagctatttg aattgcgtta gttgcagcac cttttcttat
    30001 attatcagca acaacccaaa gatttacacc attatcaaca ctaaaatctc ttcttattct
    30061 tccaacaaat acttcatctc tatcatcaaa ttctacagca gttggatatt cgtttttagc
    30121 aggattatca actactacaa cacctggagc ttcttcaagt gtttttacta aatcatctaa
    30181 ctcaaatgga ttgttaaact caacatttat agattcacta tgaccatttt ttactggaac
    30241 tcttacagta gttgcagtta tttttaaatc gtagttatca agtattttca tagtttcatt
    30301 taccattttc atttcttctt tagtgtatcc attttcagtg aatgaatcta tatgaggaag
    30361 acagttgtaa gagatttgat gagggtagaa tccagtagga ttaccgttca ttcctttctc
    30421 taaatcttca acacctttaa caccagaacc agatacagct tggtaagtag agtaaacaac
    30481 tctcttaatt ttgtatttat catcaagagc tttaagggga accatagctt gtatagtaga
    30541 gcagttagga ttagctatta tacccttatg attttttatg gcttctggat taacctcagg
    30601 aactactaaa ggcacatctg gattcattct ccattgacta gagttatcta ctactaagat
    30661 tccatttgaa gcagctattg gagcatactt ttcactaata cttccaccag cagagaataa
    30721 tgctatttgt atgcctctat cagtaaatga agtttccttt aattcttcta ctatatattc
    30781 tttaccacaa aacattactt tagaaccagc tgatttagaa gaagagaata aaaatagatt
    30841 ttctattgga aaatttcttt cttctaatac ttttaaaaat gttctaccta ccattccagt
    30901 agcaccaact attgctacat ttactttttt catataaatt caccccaatt ttaaattatt
    30961 aaaacattat tttagttata caagtaagat atgttaatat ttttatatat ccaaaaactt
    31021 taatattata ttaacaactt taattatata caaaaaacgt acaaaggtat actttgtacg
    31081 tctaatttat cactcaaagt aaaccctgta gctcatcacc tacaaaaata agtgacagta
    31141 ttacacatgt tatgtgcaat cccagaaaat attattgcaa gaaatatttt cttcggctgt
    31201 aaatcctttt tccaatcatt aacacctgcc agctactatt ggatactctt agttacagct
    31261 acctctaccc taggttaata ggatattaaa atttgattat tctaataata atacatattt
    31321 aagaaaaaat caacttattt atataaatat tgctaaaatt taaatccaaa atttgtcaat
    31381 tcttgtttta acacattaag attagtttct tccatttcag ctagaggaag tcttaaatca
    31441 ccaacattaa aacctaaaat gttcatggca gttttaacag gaactggatt tacttctata
    31501 aataaggcag caattaaagc atccatatca agttgtaatt tttttgagcc ttcaatatca
    31561 ccttcaaaga atttagttac taaatcatgt gtttctttag gacatatgtt agcaagtaca
    31621 gagataacac cacttccacc taatgataat agtggtagta tagtatcatc attaccggag
    31681 tatatagcaa agtctttagg tactaattta gctatttctg caacttgagc taagtctcca
    31741 cttgcttcct tcacagcaac tatattatct attttagcaa gttcagccac aacagatggc
    31801 tttatattca ctttagttct tcctggtacg ttgtataaaa taattggcaa cttaacgcta
    31861 tttgctattg tttcaaagtg aagtttaaga cctttctcat tagtcttatt atagtaagga
    31921 gttattatta aaagaccatc cacccctaat ttttcagctt cttgactcat atgaacagac
    31981 aacatagtat tgtttgaacc agtacctgct ataacaggta ttctcttatt aactttttca
    32041 actgtatatt ttattacagc aaaaatttca gattcactca tagtagtcgc ctcacctgtt
    32101 gttccacaaa caactagtgc atctgtgcca ttctcaatgt gatattcgat taattcacct
    32161 aatttttcaa agttgacatt attatcctct gtaaatgggg taactaaagc aacagcagaa
    32221 cctttaaata acatataatc aaatctcctt ttttatctaa atttaatttt ttaagtacaa
    32281 taataattat agtacaaatt aattaatttt aataggtata gttaatatct taactgttta
    32341 aataagatat ttatattata ttaatattag aaaaatttta aataaatgtt ttattctcaa
    32401 aatatgcata ttaatttaat aaaatatacc aaaatggaaa taaaaaataa attttatgag
    32461 aatttaataa ctaataatta atatatttag gttataataa atatgtgcgt aaaatctaac
    32521 aataagcaat aataccgtaa atttaggagg aataatatgg atactttaaa ggaacttttt
    32581 aaaataggaa gtggaccttc aagttctcat actatgggac cacaaagagc agcagagaga
    32641 tttaagaatg aaaacccaga tgcagaaagt tttagagcaa tattatatgg tagtttggca
    32701 gccacaggga aaggtcattt aactgactat ataattgaaa aaactatagc acctaaaaaa
    32761 gtcgaaattg tttgggaaga agatattata aaagattttc acccaaatgg gatgaagttt
    32821 gaagctttag ataaagataa aaatgtaaca gctgaatgga ctgtatattc tgtaggtgga
    32881 gggactatag cagaagaagg tcaaagaaac agtaaaagca atagtatata tcacttagat
    32941 actatggatg agatagtaaa atggtgcaaa gaaaataata agactttggt agattttgtg
    33001 ttggaatgtg aaccaaaaga cataaaagat tatataaaaa ctataaaaga tgctatgaga
    33061 aaatctattg atgatggact aagtacagat gaaataatac caggcaagtt attattaaaa
    33121 agaagagcaa gtacatttta taatgcatat aaaaaagata agagcttctc aacacttgta
    33181 tatgcatatg cactagcagc ttcagagcaa aacgcttctg gtaatataat agttacagca
    33241 ccaacttgtg gttctgctgg agtaatacca ggaatattct ttgcaatgca ggatttctat
    33301 aattatgatg acgaaaaaat aattgaagca ctacttgtag ctggaataat aggtaatata
    33361 ataaaaacta acgcttcaat atctggagca gaggtagggt gtcaaggtga agttggagcc
    33421 gcttgttcta tggcagcagc agcagtagca tacttaaaag gtggaactat agaccatatt
    33481 gaatatgcgg cagaaatagc acttgaacat catttaggaa tgacttgtga ccctgtatat
    33541 ggatatgttc aaattccatg tatagaaaga aatgcaatgg cagctcaaag agcttatgat
    33601 gcagctaatt atgcattatt aactgatggc tcacattctg tctccttaga ccaagttgtt
    33661 gaaacaatga aagaaactgg tatagatatg atggataagt acaaagaaac agctaagggt
    33721 ggacttgcta aacatttctt ctcttgctaa aaataaaatt ttccaataat aattcctctg
    33781 ttttgattta tgaaacaggg gaattattat taatggtgaa ttatttaata atgcaaaata
    33841 tttttatatt caatttagct ttttattttt aattagtata acatgctata taatatttta
    33901 attataaaga aatatagaaa tattttttaa taaattagaa attggaggag attacatgag
    33961 agaggcatca tatttacaag aacaacttta taaacatagg aagtcattaa atcctattgc
    34021 agagattggg agaaaagagt ataagacttc aaaatatata agagattatc ttgataaatt
    34081 aggaattgag tatgaagttt atttagaaac aggtatagta ggtgtaatag aaggtaaaag
    34141 acctaaaaaa gatattgctt ttagagctga catagatggt cttcatagtg aagaaggggt
    34201 aaaacattta tgtggtcatg atggacatgc tactatatta cttggattaa ttgagtattt
    34261 aaatgataat aaagaagact taaatgataa tatagtattt atattccaac cagcagaaga
    34321 aggtcctggg ggagcaaaag atttaataga tgaaggagtg cttagaaaat acaatattga
    34381 tgaaatatat ggtcttcata tatatccaga gttaccagaa ggttatgtag gtacaagaga
    34441 gggctatttt atggctcaaa taggagatat tgacatagaa atagtgtcta aaagtggaca
    34501 tggagctatg ccacaaaatg gtattgatgg tatagtgata gcttcaaact ttgtaagctc
    34561 acttcaaact atagtgtcta gaaatataag cccaatagac aatgcagtgt tgactattgg
    34621 tagaattgaa gggggagcta gaagaaatat aatagctgaa aatataaaga tggaaggtac
    34681 tatgagatgt ttcagcccag aagtgtatga gaaaatgaag cttagagtta gagaacttgc
    34741 tagagggttt gaattggctt ataactgtaa ggttaatata aatattatag atgattatat
    34801 cgctgttaaa aatgacaagg atttgtatca agagtttgta gaggctattg gaagtgatac
    34861 tgttgtagag ttagagccac ttatgatatc agaagatttt tcttattatc aaaaagaagt
    34921 tccaggatta ttttttatgt tgggttcaag aaacgaagaa aaaggatttg taaatggtct
    34981 tcataatata aactttaatt ttgatgaaaa gatatgtgta aatgcattga atgtttattc
    35041 aaaattatta aaatacaaag aagcaataga ttaggaagat aagttataaa gattaaaaag
    35101 cataaatacc taaatttaaa aaaagtgtat taataaatac cccttacata tttgtataaa
    35161 tttattttaa ttgacaaaaa tacaaataag aaggggtgat ttttttgaaa gaaaaagtac
    35221 aaagagaaaa gaagataact aatggcaaga atactaaaat attgacaaat gatgatataa
    35281 tgaaatatga gatagcctca gaactaggtc ttatggataa agttggtgaa cgtggatggg
    35341 caggacttac agctaaggaa gcaggaagaa ttggaggtat gcttacatca cgcaaaaaga
    35401 ggcttaaaaa gattgtggag gagcagaata aaaaggatga gtcaatatag tgattttgcg
    35461 tttgtatatg atgaacttat gaatgaagtt gattataatg gttgggttaa atatatagaa
    35521 gatataatag atagtgaagg tgtaaaagta aaaaatatat tggaattggc atgtggcacg
    35581 ggtaatctta caattccatt gactaaaaaa aattacgata tagctggtat agatatatct
    35641 gatgagatgc ttagtgtagc aagagaaaag gcagaaaaag aaggagtaga attagtttta
    35701 cttcaacaag atatatcaga gcttgatttt gaaatatctg atttggactg tgtactttgt
    35761 gcttgtgatg gatttaatta tataacttat gatgatgatt tggaaaatgt attttcaaag
    35821 acatatgaat tattaaagaa agatggaata ttcatatttg atataagttc tttttataaa
    35881 ttagccaaca tattgggaaa taacatgtat ggagaaaata gagaagatat agcgtatatg
    35941 tggcaaaatt actttgatga agaagagaat ctaattgaaa tggaacttac attctttatt
    36001 aaagatgaat gtggaaagtt tgaaagattt gaagagatac accaacaaag agcatatact
    36061 gaagatgaga tagttgatat gcttgaaaaa tctggattta aggatataaa atattatggg
    36121 gattttacat ttgacaaacc tagtgaagaa agtcaaagaa tcttctttgt atgtaaaaaa
    36181 taagatattc ttgactatat aagataatta ttaaaaataa ccatgatata aaaatatata
    36241 aaatgcagtt ttatagatta aaagtaaagt gatgatattt tatgattttt attttctaat
    36301 aattataaag ttttatagat aaaaattaaa atataataac tattgcttag taaaattaaa
    36361 ataaaatggg ttttgcccaa ggttaagata gataagtatt tatagattaa aattaaggta
    36421 gtaatataat aaaaataaga caattaacgg gacatggagg aatattaatg agagattatg
    36481 tactaagagc gacatcagga aatggtcaag ttagagcatt tgtcgcaact actagaaata
    36541 cagtagaaga agctagaaga ttacatgaaa ctacaaaagt agcaacagca gcacttggaa
    36601 gaacattaac agccacatct ataatgggat taatgatgaa aaatgatagt gataaattga
    36661 ctgtaataat aaaaggtgga ggtccaattg gaactattat cgccacatca gactctaagg
    36721 gaatggtaaa aggatatgta ggaaatccac aagtagaggt tgaagactat ccaaatggaa
    36781 agttaaatgt agcagcagca gtaggtacag aaggtgttgt aaaagttata aaagatttag
    36841 gtcttagaga accatataat gggacttatc cattggtaag tggagaaatt gcagaagact
    36901 ttacttatta ttttgctgta tcagagcaga ctccatcagt agtagcttta ggtgttctta
    36961 caaaagaaga tgaagtagag tttgctggag gatttatagt tcaattaatg ccagatgcag
    37021 aagaagagac tattgctaag ttagaggaaa atgtagctaa attaccttct ataactaaca
    37081 tgttaaaaga aggaaaatca ccagaggata tattaaatat tgttttggat ggtttggaac
    37141 ctaaaatact tgatacctgt gaagttgggt ttatgtgtga atgttcaaaa gaaagagtaa
    37201 aaactgcttt ggttgcaatt ggtaaaaaat cattggctca aataatagaa gaagataaaa
    37261 aagcagaagt tggttgccag ttctgtaata agaagtatat gtatagtgaa gaagagttgc
    37321 ttgaaatttt aaaggaaatg tagactataa aaatagttgt attgatataa gtaatgttat
    37381 agtgatatag taaagtcgta ttgatatagt aattaaagta taatttatta ataatagaca
    37441 tatttatatt aaaaaataag tttaataaat ttttaagctt aataaatttt aagaattaag
    37501 aagctttctg gctgtaatgt ttgcaatgtt gcagttagaa ggctttttta ttttattata
    37561 gttcgtgtca aagattgtat gaaacaataa aatattgtaa aataatttgt atatttagac
    37621 ctgattgaca caaaggttta taagatattt gagttgatta tacgaaattc tatgaagtag
    37681 taaccctgtg actttaatca tgggaggttc agaagaagga gaaaatttta aatatggaaa
    37741 gtaaaacgaa gtacatagga ataagtatat taatattatt gatgatattt ttaattgcaa
    37801 agaattttaa cggaaaactt gaaataccta ttcctgagag taatagtaga aaatttaaat
    37861 cagcagcagt gttttatcct caacatcaag atgatgaggt tttatgggga ggaagtgcca
    37921 ttgtagatgc tataaaacag tgtggtgttg ataatgtata tgtggtatta gtatcagatg
    37981 gttctggagt taacgttttc aaagctaaca caaaatttag aaatttaact aggaaacaaa
    38041 aggaagagct tagaaataac gagtttaaat cagctttgag agagcttgga gtgaaacctc
    38101 aaaatgtgat tatacttgca gatatagata aaaaggaagg cacacattat gaattaatgg
    38161 aaaagactat attggatttt gaacataagt ttaaaggcaa tgtgacccat atagcccatc
    38221 actataagta tgatgaccat ataatgcata gaaagaatgg ggaagtttta aagaaactta
    38281 aacgtgaggg taagataaaa gatgacttgt attttgttaa gccaaaatat gtaaagtata
    38341 taccaagaaa tgaaagagct atctataaag caatggattt tgatgactac aataaggtta
    38401 agagtgcttg ttatgaatat aaggttgtaa attcaaagcg taaaatgtat ggtataggat
    38461 atatttcggc acatagttat tttgaacatc ttctaaatga ccctgaactt acgtctgtat
    38521 taagtgaaga ttaatttctt ataagtagta agttatgttt gataagttga ttggaatttt
    38581 ctatagtttt gaaggtattg gaaatttagt tttatttaaa gatttaaaga tagaggtttt
    38641 tagtgtttaa aattttaaag ttaagttgaa taattgtata gaattatttg tgaaagaaga
    38701 aacagtgtaa aacatagtat cttatttatg gttttaaaat cataggcaag atactttttt
    38761 tatgtaacta ataccatttt gagattatta acttaagaat tttatataat ttaacactca
    38821 tatataatga tgatatttag gtagaataga tgtaattacg cttaaataaa ataactgtat
    38881 tttttataaa aatccaaacc ctacactata ctaccagata aaatctacat gctataattt
    38941 gacctgtgtt tataaaaaaa ggagtaatct aataaaatag tatttcactt atgtataact
    39001 aacaactatt attacgattt tctctaaata atttaaagaa aaataatgca ggaggtaaaa
    39061 tatgaacaat gaatttataa ttttaaaagg ttgcaaagag aacaatttga acaatatttc
    39121 cttaaaaata ccaaaaagaa aaataaccat attcacaggg gtgtcgggtt ctggaaagtc
    39181 atctattgta tttgagacaa ttgcaaaaga atcacaacgc caactaaatg aaaggtttag
    39241 tacatttgta agaagttttt tacctaagta tggagaggtt aaagctgatt gtatagaaaa
    39301 tttgtcaaca ccaattatca tagaccaaag tagacttggt ggaaattcaa ggtcaaccct
    39361 aggtactatt acagatgtaa attccttttt aagagcttta tattcaaggt ttggtagtga
    39421 gtatataggt aatgccaata tgttttcttt taataatatt gatggtatgt gcccacactg
    39481 tcatgggctt ggaaaaaagc tagttcctaa tatgaaccaa attcttgata tgaataaatc
    39541 attaaatgaa ggtgctatat tgctttcagg gtttggagtg ggttcttggc attggaatat
    39601 attcgctgaa tcaggatttt ttgatattaa taagaggata tgtgattatt cagaagtaga
    39661 attggaaaag tttttgcatg gtaaagcaga aaagattaag atagaaaatg ctggtcaaac
    39721 taacatgaca tatgagggac taatggttaa atttaatcgc ctatacttaa gtagagaggg
    39781 agaaatatct gaagctacta agaaaaaatt gagtaaactt cttatagaag atagatgtcc
    39841 tatatgtgat ggaagaagac ttaatgaaag agtatataag tctttaatta atggttataa
    39901 tatagcagat ttaactagta tgcaaattga tgaattggct gaagttatta aaagtataga
    39961 tgagccagaa gcacaacctt taattaaagg aataatagaa aagttaaaca gtatcataga
    40021 tataggtctt ggatatttaa cactggatag agagacatct tctctatcag gaggggaatc
    40081 tcagcgtata aagatggtta agtatttaaa tagtaatctt gttgatttaa tgtatatttt
    40141 tgatgaacca agtgtaggac ttcatccaaa agatgtgtat aaattaaata atcttttaaa
    40201 aaaattgcgt gataaaggga atacaattat agttgttgaa catgacccag atgttattaa
    40261 gattgcagac catattgtag atgtaggacc aaaggcaggt aagtatggtg gagaagttgt
    40321 gtatgaaggc agttatgaaa atctacttac aagtggtact ttgacaggga atgctttaag
    40381 taaatttctt cctattaaag aaagtgtaag agaacatagt ggatatttag aagttaaaaa
    40441 ttgtaataaa aacaatttaa aaaatgtatc tattaagata cctaaaggtg tattgacttt
    40501 agttactgga gtagcaggag caggtaaaag tacattgatt aaggatgaat ttttaaagca
    40561 aaatcctagt gctgtactta tagaccaaag ccctgtttca gcaaattcaa ggtcgagttt
    40621 ggcaacatat agtggaatta tgaacaacat aagaagcata ttttcaaaaa caaatggcgt
    40681 aaatgcttct ttattcagtt caaattcaga aggagcttgt gataactgta agggttctgg
    40741 aatagtagaa atgaatcttg cttttatgga gagtataaag agtacctgca atatttgtga
    40801 aggtaaaaaa tttaaaaaag aagttttaga atataggttt caagggaaga atataattga
    40861 agttcttgaa atgtctgttt tagaggcaat tgagtttttt aatttaaaac agattaaaac
    40921 gaaattacaa tgtattgaaa aaatgggtat tggatacctt actttaggac aaactcttga
    40981 tactttgtct ggaggagagt gtcaaagatt aaagcttgct agtgaattaa ataaggaaag
    41041 ttcagtgtat atattagatg aaccaacaac aggtcttcat atggctgata ttgagaattt
    41101 tataagtata attgaagaga ttgtagataa tggaaatacg gtcataataa ttgaacacaa
    41161 tatagatgta attaaaagag ctgattggat tattgagctg ggacctgaag gaggaacaaa
    41221 aggcgggaga gtaatatttg aaggtattcc aaaacaatta tgtaacaata aaatatcatt
    41281 aacagcaaaa tatatagtat aataaataaa atataataaa attataataa tattttaata
    41341 aatataatgg gtatataaaa tgataattaa acttaacagt aataaataat gtgagaaata
    41401 ttcaaattta attaaataaa gtgtcagaaa tttgtttaaa ttttagtatt ttaacggttc
    41461 tgaccttttt tcttattttt atggagaata tcgaggatgg agtctctaat agttactttt
    41521 ttgtgtctac ttgcaagttt gattaaaaaa tggctataca cacttgtgac ttcagtcatg
    41581 ggaggttccg attataatct taatatataa tttgtgaaat aatttgatga cgatttgctg
    41641 atatataaac aataaagatt aactacatta aaatttaaat cttgttaagt aaaacttata
    41701 aaaggagaga gatggatgat aaatgatatt ctaaccttat tatctgaaag aagtgatttt
    41761 tttataaagc tatccataga acatttgatg atttctatta tttctataat aattgcgatt
    41821 gttttggggg gtataattgg tattattgta agtgaatttc ataattcttc taaacctata
    41881 ttgggaacta tcaattttct atacaccatt ccatctattt ctatgctagg atttctgata
    41941 ccattttccg gaattggaaa tactactgct gttattgcac ttaccattta tgcattatta
    42001 ccaatggtta gaaatacata tactggaatt actaatgtgg ataaaaatat tcttgaagca
    42061 gctaggggaa tgggaagtac agattttcag atattgtata aaataaaact tccgttagcc
    42121 ctacctgtta ttatgtctgg tattagaaat atggtgatta tgacaattgc attagctggt
    42181 atcgcttcat ttataggtgc tggaggccta ggtgtagcca tatatagagg aatcacgaca
    42241 aataacacaa taatgacaat tactggaagt atacttattg ctctattagc tttaatattt
    42301 gattttattt tagggctggt agaaaaattt tttcaacgaa aaagaaaaaa gaaaaatagg
    42361 aaaaaagttt ttgccattct aggaagcctt attcttatag taactatctt tacgtcttat
    42421 ttatttaatc cttcaaagac tcaaactatt catattgcta ctaaacctat gactgaacaa
    42481 tatattcttg gtgagatgtt ggatatttta attgaaaaaa acacaggtct aaacgtggaa
    42541 attacacaag gtgtaggagg gggaacatct aatatccagc cagctatgga gaatggagaa
    42601 tttgacattt atccagagta tacaggtaca ggctggaata tggtacttaa gaaaaatgaa
    42661 ttatatactg aaagtatgtt tagtaaattg cagtcggaat acaaaactaa acttaaaatg
    42721 caatggactg gtatgtatgg tttttctaat agctatgcat tagcagttag aaaagaagtt
    42781 gcagataaat acaatttaaa aaatataacg gacttaaaag atgtatcttc taaattgaca
    42841 tttggagctg aatatgactt ttatgagaga gaggatggat atactccatt atgtaataca
    42901 tatggactga actttaaaga tacagttgat ttagatattg gattaaaata ccaagctatt
    42961 aatgaaggta agattgacgt aatgcctata tttacaactg atggtcagta tagtgtttca
    43021 gatgttgtag ttctaaaaga tgataaaaat ttctacccat cttatcaatg tggaaatgtt
    43081 gttcgtttgg aagtattaga ggaaaatcca gaactatcaa aggtatttaa gatggttgga
    43141 gatattttga ctgataaaga aatggctaaa atgaattatg atgtagaaaa caacaattta
    43201 gaacctagag aagttgctcg tgagtttctg aataagaaag gtcttttata aattggagga
    43261 aatataatga cacctattat acaatttaaa aatattaaaa aacaatataa tgataaaact
    43321 ataatagata atcttaattt agatatagaa aaaggagcgt ttttgactgt aattggttcg
    43381 tctggttctg gtaagacaac actcttaaaa atgattaatg gtctgatttt gccagatgga
    43441 ggaaatattt tgataaacca aacagatatc aaaaacgagg acttaattaa attaagaaga
    43501 agaataggat attgtgtaca gggaagtgtt ctttttcctc atatgactgt tgaagaaaat
    43561 atttcatatg taccaaatct cctaagtaaa aagaataaat tagaagtaaa aagtgctgta
    43621 aacaagtgga tggaaatagt gggattgcca aatgatatga aagtgcgtta cccatcagaa
    43681 ttgtcagggg gtcagcaaca acgtgtaggg attgcaagag ctttagcttc ttctccagaa
    43741 attttattaa tggatgaacc ttttggtgca gttgatgaga ttacacgaaa gcaattacaa
    43801 aaagagataa aagaaattca taaaaaaaca ggagttacga ttatctttat tacgcatgat
    43861 atttatgaag ctttgatttt gggaacaaag actttggtat taaatcatgg agttgttcaa
    43921 cagtatgata ctcctgagaa tattttaaat actcctgcta accaatttgt agaccagtta
    43981 ttgaatataa gaaaatctat aatagatgat gataatttaa aagaataaat ttaaatttaa
    44041 aatcaaattg ttgtgataaa aactattgat ttagagctaa cttcaagttg tatacttgaa
    44101 gtggaaaagt tagattaagg tatagaaggg aatttagcag ttggctgtag acaaggtaac
    44161 tgctgaaggt gatttcaata aatggctgga acctgtatct gttgaagatt ataataaatt
    44221 gaaataagaa gctcaatata cagagtagat aggcacttca aatagaagtg tcttttgtgt
    44281 gccataaaat aaatcaaaat attagcaaag aatgtgaaaa acaaaataaa ataatatgct
    44341 atctttaaaa taggaggggt gctatatgga taacaatttg attaccattg aaaaaacttt
    44401 agattatata gaaaaacatc ttttagatga gatgttagat ttaaatgtaa tttctaaaga
    44461 gataggctat tcaaaatacc atttacatag aatgtttaca tcagttgtcg gatttagtgt
    44521 acataattat atacaaagac gtagacttac tgaagcagcg agaatgctaa tttttacaga
    44581 aaaaccaatt attgagattg ctttaatctc ggggtatgac acacaacgct ctttttcaag
    44641 agggtttaaa aaaatgttta aacgtagtcc taattttttt agaagacatc aagatttttt
    44701 accactacaa ttaaaatttg atattcataa tagaaaaaaa ttacgtggag atatggtttt
    44761 agatgttaaa aaagttaatt gtgcagatat aaatattgtt ggctattctg gtaaagtaag
    44821 aaaaggattt catattattg gaaaatgctg gagggatttg cataaaaata aagagatgat
    44881 agaaaataag gtagatttag gttttttaat tggagttagt gattatagca attttaaaga
    44941 gattgagaat actcctacat ttacatacat tgcaggtgct caagtagcta cttttggcaa
    45001 attacctaat ggaatggaaa aattcgtatt accgaaaagt aattatattg tttttacttt
    45061 tattgggaaa aatgaggata gtatgcaaaa tgttgtagaa tatatctatc aagaatggtt
    45121 ccctaattca agttgccagt ttaatgaaaa taatttatat gactttgtaa aatatggaga
    45181 aaaaactgat gagaatggtt tgagtgaaat tcagttttgg gttccaatca tttaaaagaa
    45241 ggtattatgt ataaagtaga tggatttata taggcaaagt tttacagcag tatgtattta
    45301 taaataattt atagtttgta ggaaagaaat aattttacta taagattatg ataaaataca
    45361 caatactatg acaaaatagt gttaaataat agatttaatt ttgcatagga aaagttctat
    45421 aatggtgtgt attcataaat aatttataat ctgtaggaaa gaaatagttt cactataaga
    45481 ttatgataaa atacacaata ctatgataaa atagtactaa ataatagatt taattttgca
    45541 aaggataagg tgttttaata aaaatgaatg tagaaaacga attacttaaa aacctagata
    45601 gattgcatac aacagagtta ggcgttgtaa gaattaagaa aaatctttct ttggagacaa
    45661 atgatgtagt gaactggtgt aaaactaaaa tagaatctcc aaatgccatt attaatagaa
    45721 agggaaaaaa ctggtatata agtgtatatg attgtattat aacagtaaac gctcacagtt
    45781 atacaattat tacagctcat aaagaaaaga aatgaattgc aaaatacaaa ggtcaaaagc
    45841 attttccact gtttgtagta cctatgaaga agtatataaa ataatattac ttttgaataa
    45901 taatttaaat tttggaggtt gaaaaaatga atgatactgt aaaagaaaaa ttggcagagt
    45961 cactaacttc tgatactact gatattttac cttatttgcc ttatttatta caagattttt
    46021 gggaattagg aagttcacca gaacaaatgt tacaattatt tagaaagcat atacctttta
    46081 atgaagatac aaaagttttg gatttagctt gtggaaaagg tgctgtatct gtgaagcttg
    46141 caaaagagtt gaatatacat atcaaaggta ttgatttgat acctgaattt attgattatg
    46201 cgagaagaaa ggctattgaa tataaggttg atgctttgtg tgattttgaa atgaatgatg
    46261 ctaatagttc tgtaaaaatt gagcatgatt atgattgtgt gatttttgga gctgtagggg
    46321 atattttagg taattataaa gaaactataa gtaagctatt acctacaata aaaagtaagg
    46381 ggttcatttt aattgatgat gcatatgtag tagataaatc tacaaatgaa cagcttaatt
    46441 ttgagagggt gtatcccact tacaatgagt ggtgtatatt gttcaaagaa ttaggtctga
    46501 agctaattga atgtatacca tgtttagaac ttgaaaatga tgttgaagag gaaatgaatt
    46561 ggattataac tcgtgcaaat gagctaatta aaaaatatcc ttctcaaaaa gatatgtttg
    46621 aacaatatgt tgttagccaa aaaaatgaat atactgattt acagcaggac cttgttggag
    46681 caacttggat tttacaaaaa gaataagata aatattagac tagataatca atgttatagt
    46741 gaattatccc atgtctagtg aatataaata acataaaaaa ccgtcaagat gaatagttta
    46801 ttcgtgcttg acggttttta gtatttgttg aagtaaaatt ttatttcttt agtatatcac
    46861 ttttagtcaa tccatttgat ttagcctgag tattccattg atgattcccc ataagtttat
    46921 ataggaatct agggaagtta ggtgaaataa aaatagtact tttgttggaa tcatctatat
    46981 gtatattatc actaagtata ttttttgaaa gttcttttaa tgcaattcct aaatcttttt
    47041 tagatccttt acccaaaggt acggattcta gtgaaccaaa catctcccca gctccaattc
    47101 caataccttg aacccatgta aagttgattt ttttacacca atgctctatc atttgaattg
    47161 ccaaatgatt atgttcacct tcattgaatc cacagttaac tattgcatac actggaatag
    47221 gttttttatt ttgtgttttt aaaaattttt cacatttctc taaaaaatga agtaaatgag
    47281 atggaatacc atctacataa agaggagatg cgattattaa tctatcacaa ttaagtatgg
    47341 cattaagagc gtcaggatac ttagatgtgc tatctacttt aaatgtaaaa actttattat
    47401 cattttgaat aagttgtttt aattcagata aaaaatactg tgaattattt ttatttactt
    47461 ttggactacc atttatcatt actatattca tatggattca cctcttatat cttgtatatt
    47521 ttcatagaaa caaacagagt gttcttttat attgaaattt atagcatttc catttactaa
    47581 tttcgtagca gtttcttttt cagcttctgt aatacaatct ccatagaata tagcttttaa
    47641 atttatctgt ttatcatatc tgcttttatg acgcatcata ttatctttta ttacaaagta
    47701 tggaagaata taattaatgc ttttatcaag tacgtttttg acaaaagggc taaaccctcc
    47761 gtaacagcat ctgcttatta taataattcc atcagattca tttataagtt tacccatgtt
    47821 gctgtaataa tcttttacaa cacattcaga aggagtttta atccagcaac caaaacaacc
    47881 aatacatttg tggatttctt ttgtgtttgg aagtattttt atattgtcat caagtttggg
    47941 taataacttt tctaattctt tgtcagttaa atcatgtatt aaaagcctca taaagtccat
    48001 ctccttttat tatgtatgtt tacttctaaa aattatatct tatagatttt tcagtaaatc
    48061 tttacaccaa ttaataacaa aattataata gtcaactcca taaatcattg ttgcatacat
    48121 atatgtatat tggtcaacac aatgattttc ttctgcatca tttttttgtt tacttaattt
    48181 atttagaagt aattctactt cagttattaa catttttaaa tttgtttcaa ttatttcttt
    48241 gggtagatac ttgtaaaaga atatattaac aaggtggtct tgctttgttt tagaaaaatt
    48301 tataggtttc tttaaccatt caagaaatac ttctttacca gtattagtta tggaatatag
    48361 tttttttaat ttgctaccat ctataacttc gtgatagtgt atatatcctt ttgtttctaa
    48421 tcttttaagt gcaggataga tacttccaaa gctggcgtca aaaaagtacg aggtactttc
    48481 tgacattatt tgttttaagt cgtatccact taattctttt tccattaaaa atcctaaaat
    48541 aatgtattct aacatattac ctcctagtat caattagata tatatcgatt cgatatattt
    48601 attattacag ataataaatc ttacgtcaag atgcttttta aaataagcta aaaagaatta
    48661 ggagttattg ataaatatgc aaaaaaatta atataaaagg taattaaata tcaatgaaga
    48721 tttaaaaata gttaaatcaa agtaagagtc taatatataa aatttatagt aactttatgg
    48781 tttaaatata aacttgtgtg ctattgacaa agtataaaat tagcagtaaa ataattgcat
    48841 gagcaaatag attattgcga acgcaaaaaa ataaggacaa tagatattaa aggagagaat
    48901 atgagagagc tattaaaatg ggtttccatt ataaatagat acaatacaaa ttatattgac
    48961 aggaaattgg cggatattgg aatcaataat agccaatatt tttatgtatt acacatatgt
    49021 aataatccag gtattacaca ggacactata tttgagaaaa ttcttgtgaa tcctagtaat
    49081 ataacaagag ctttggcaaa tttagaaaaa gaaggcttta taacaagaga accaggtgta
    49141 aaggataaaa gaacatggca tttatatcct acagaaaaat ctttaggatg ttatgatgaa
    49201 atactaaaaa taacaaatgg gtatgttgag gagttgttga gttcatttga gaaagaagag
    49261 caagagttgt ttatgtcaat gttaaagaaa gttgcatttt tggctattga tatgaatgaa
    49321 gaagcaaaat tggaggcgaa ggaaaatgga agaaaaatat aaggagccat tggaatatga
    49381 aaaggtttca aagttattaa ctaagtacgc aattcctagt attatagcaa tgttagtaag
    49441 tgctttatat aacattgttg accaaatatt tattgggcaa ggtgttggtg tattgggtaa
    49501 tgctgctaca aatgtcactt ttccactaac aacaatatgt accgcaattg cattattgtt
    49561 aggaattgga ggagcgtcta agtgttcatt ggaattaggt gctaaaaatt cagaaaaagc
    49621 tacaaaagca gcaggaaatt ctatatgctt gatggctgtt tttggaataa gtttatttat
    49681 agtagtatca atatttttaa caccactatt aaaattcttt ggctctacat cagaaatact
    49741 accttatgca cacacatata caaaaattac atcaattgga ttaccatttc ttataatgtc
    49801 cactggaatg agtaagttga tacttgcaga tgggagacca aaagcttcta tgttttgtat
    49861 gttaatagga gcagttatta atacaatttt gaatccatta ttcatttttg tttttaatat
    49921 gggaattgct gggtctgctt tagcaactgt tattgggcaa cttatatctt ttggaataag
    49981 tgttcattat ataaagaatt ttcaacatat taaattacat aagaaaagtt ttaaattaga
    50041 tagtataatt tataaacata tatttgcgct aggttcaagc tcatgtttta atcaattagc
    50101 aatgactgta acacagatag ttatgaataa tacattggca tattatgggg ctaggtcagt
    50161 ttatggaagt gagattccac ttgcttgtgt cggtatcatt attaaagtaa atatgatttt
    50221 tatatctata attattggta tatcacaagg tatgcaacca attatagggt ataattatgg
    50281 ggctgaaaag tataatcgtg taaaagaagc atataagttt gcagtgggaa gtgcaactat
    50341 tatatctgtt attgcattct tatgtttcca attcttccca agacaaatta cgtctatatt
    50401 tggtaacgga aatgagactt attttatatt tgcagaacat tattttagaa ctttcttgtt
    50461 cttaacattt ataaatggtg ttcaaatagt atcatcaact ttctttacat caattgggaa
    50521 atcagtgttg ggacttttta cttctttgac tcgtcaaatt ttatttttaa ttccattgat
    50581 agtaatttta ccaatgttta tgggaattga tggagtaatg tttgcaggac caatagcaga
    50641 tgcagcagcg gcaatggtat gtatatactt tgcagttcat gaaatgaaat cattgactat
    50701 aaagcaaaat aatttgatga gtaaccataa taagattgca gaggtataag acaatattta
    50761 agaagtatag aaaattaatt taaaaaatat aataaattaa tttaggaagt gtagaaaatt
    50821 aatctaaaga agtataataa actaatttag gaagtataga aaattaatct aaagaagtgt
    50881 aataaactaa tttaggaagt atagaaaatt aatttaaaga agtataataa actaatttaa
    50941 gaaatataga aaattaattt aaagaaatac aatacattaa tttttaagta acattaaatt
    51001 aatgtaagta aaataatgtt catatgaata tttaaaggtg cattttctga agctaagtat
    51061 atgttgtaga gtgttatgtc tctctatcct gtgaagttta atagctcaag aaaatgcact
    51121 atttatgatg attattttgt tttatttcca attttggaaa taactattct aattaaatct
    51181 aaaattatac cataaataag aaaagaaagt atagatgcta tgtaccaata aatcgaaatt
    51241 gaccctgtaa ttgttctaca tgggatttgc atacctgaat ccataaaaac aaaaaaacta
    51301 tcaactctgg aaataataga caaaatagga tttaaaccta ttagaacaat actttttgag
    51361 tcttgaccaa tttgatgcat ataaatctca aataatgcaa ttaaaaaaaa ccaagatgta
    51421 aaagatttaa taattttttt catcaaaata cctcacataa tataaattct gtttgcacta
    51481 tttgcttact acaactattt atctccattc agtcacatca cttaattgta atctaggtct
    51541 tcttgatggt tcttcatcag gataacctaa agatattgct gccactaatt gactatctgt
    51601 atttaaccat tcacataatt cacgatatgc aaagtataca tcacaaatcc aaagactacc
    51661 tagacctaat tccacagctg taagcgacat attctgaatt gcagctccaa ttgattgcat
    51721 atttgccatt tcatagaatc gttcttcagg ggatagcttt tctaaaggtg atttacctaa
    51781 tatatttaaa ataaaaattg ttactggagc ttgtttcatg atttcaactg tgtaatttgc
    51841 accagcgata tgttgacgac ttcctggaag aagcccatta tcattaattt cattttgaat
    51901 acctttgctc atggctttaa gcatagattc tttttccttt tcagtgatga caacaaaacg
    51961 ccatggttgc ctgtttttag atgaaggtgc atttattcca gcttcaatta ttttctctat
    52021 agtttcatgt gaaatgcttt gatttttata ttttctgata cttcgtcttt ttgatataga
    52081 atcacttatc attttaagta acctccgttt attttttgga aaaatctatt taatttttat
    52141 tttctatttt aattatttta gcattaatca gaaaaaaagt cattgatttt agtatacaat
    52201 tattaatgat tatattaaga attgtatcaa aaagtatgtg attatactaa gggattatat
    52261 taaacattat atttaaaatt gtatgaaagg ttgtatttaa tattatattg agagatatat
    52321 atattaggat ttatatatat tcagatttat tattatattg agtcttatat taaattaagg
    52381 actataattt gaaaataagc tataaaaact caatattgtt ttgttcaaag ataaaaaaag
    52441 aatactttag gtgattttta ttaaaaatta agagtgataa gattatatac tttataaatg
    52501 taaatatgac ggataaaaat gataaatata ataatatata tatttacatt aggagttttc
    52561 tatactataa ttatttaaaa ctaaatataa atgttggaat tatttaggac ttgaaattgt
    52621 ctaaaaataa atataagtga tgaagaggtg atgtgatgag attagatatg gatagtatgt
    52681 tatatgtact aatttatatg acattattgg tatctatcat tggaagtata ttttattttt
    52741 gtaaagacct cttaaatcat aaaattaagt atgttttaat aagagacata tttgtaaata
    52801 gaattacata ttctgaagaa cggttgccta aacagtatat agtttttcag aaatatgata
    52861 tttggaggta ttgtagtttg ttttaatttg aaatgaatct attataaatt ctaaagttta
    52921 aaaataaaaa ggagagaata aaatggattt catttcaagt atattgcaag gattattaag
    52981 tatattattt aattttacag gtgattttgg aatttctatt gtacttataa ctttaatagt
    53041 taaattagta cttatgccac tatcattaaa acaaagattt tctgtaaaaa aacagcaaga
    53101 attagctgaa aaaatggaac atataaaaga aaagtataag aatgatacta aagaattgga
    53161 aaagcaaata caagtaagtt ctgtagaaag tatgaagagt atgctgggat gtcttatagt
    53221 tttggttcaa atgccagtag tatatgcttt atatcatgta tgtttagata tgacaaagga
    53281 atttacaaca attataattc catggattgc taatttaggt acatttgata atttgtttat
    53341 attaccttgt atatatacat taattatgtt agcttcaaat ttagtgaatt atataccata
    53401 tttaaaaata aattctcaag taaagtttaa caaacaaatg gctgtggtga ctaccataac
    53461 aagtcttata ctaactgtta gaactccagt agccataggt ctgtacttta taacaagtgc
    53521 aatttattca ttaattgaag atatttgttt tagaatatat tttaatcgaa aaaatagaat
    53581 agtgacaaaa taatataatt attattagaa atattttagc ctgctaacat agaaaaagtt
    53641 agtaggctat ttttatctat gatatcaatt ccattaagaa tagtctaaaa aatactatca
    53701 aaatgctata aaaaatacta tcaaaatact ataaaaaaat agttgaaaaa taaataaatt
    53761 aatataaact aaaataaagc ctacaaaaaa gaggtgataa atagaaatgg ctaaaatact
    53821 aattgtagaa gatgaaaaaa ggatgcaaag tattattgta gaatatatgc aaaagggagg
    53881 atatacttgt ataactgctg atgatggagt cgaggcactt acaatattaa agagcaacaa
    53941 tatagattta atgatattgg atattatgat gccgtattta gatggatttt cagtgtgtag
    54001 agtttcacgt gaaatgacta atatacctat tattatattg acagctaaag gtgaagagga
    54061 ggacaaatta aagggttatg aatatggcgc agatgattat ataacaaaac catttagccc
    54121 caaagtactt ttagcaaaag ttaatgcctt gttacgtaga tacactactg atattcccaa
    54181 aaattcattg agtttaggaa agatttttat tatggtagca tcaaggcaag tgtatgtaga
    54241 agataaattg attgatttaa catataaaga atttgaatta ttgcgtttat ttatggaaaa
    54301 ccctaatcaa gttttttcca gagagaaact tttaaattgt atatggggat atgattttga
    54361 aggaaacaca cgaactgtag atacacatat aaaaacctta cgcaaaaagc ttggtagtga
    54421 aggacaccat atagttacat taattcgttc aggatataag tttgaggtta aggaatgaat
    54481 agtttaagaa agattataga aaaactgaaa aatgtagata tttcaaattg gattaaacaa
    54541 aattttggac tatattcagt tagaaaaaaa gtgtttttac tttctaaatt atcaggagcg
    54601 ttaattatat tattttatct ggttgttgaa gaattttcga taaactctaa attagaattt
    54661 tggatttggt ttataatact ggttatattt attatgagta ttgatttctt attgggaaaa
    54721 tttatttcag aaccaatcac atctataaat aaatctgcaa aaagtatgtc ccaacttgat
    54781 ttttctaatc catgtactgt aaatactaat gatgaatttg gagaactttc taggagcctg
    54841 aatacaatgt ctacaaatct acaacaggca ttatcagacc tagaaagtgc aaatattcaa
    54901 cttgaaaagg atgtaaataa ggaaagaatg ctattggaac aaagaaagga gttggtagat
    54961 acaatttcac atgaaatgaa gactccactt ggcattatac gtgcgtatac agaggggctt
    55021 atggatgaag ttgatgaaga aaaaaagaaa aattatatga atgttataat agaagaaact
    55081 gatagaatga acaatatgat tgtatcatta ttagacttat ctgcattgga agcaggagtt
    55141 tctaaactta acccagagcg ttttgatttt atagagtttg ttgaaactgt ggcaggtcgt
    55201 cttttaattg atataccaga tgccaatttt tattttacat atgatttgcc agagtatgaa
    55261 gtttttgtag tggcagataa aatgagaatg gaacaagtag tagagaatct agttattaat
    55321 gcaaagaagc atgttgtata taatggtaat ttagatttat cgattacatg tgaaaatggc
    55381 ttgcttttat ttgaaattta taatgatggt aggtttatag agcaagatga gatttcaaag
    55441 atttggtcta agttttatcg tagtgttcaa tctcagcgta caggaggttc aggtctaggg
    55501 cttgcaattg tttcacaaat acttacaatg caagggttag aatatggagt gaataatcgt
    55561 gatagaggag tagagtttta ttttatgatt ccaattgatg aataatttag tattaattta
    55621 taaattataa gtatctgatg taaaatattt ttctttgtat atgtaagcat ttaaagagaa
    55681 taaaataaaa aaacttaatg aaaaaagctt aatgaaaaag tctaataaaa agcttaatga
    55741 aaagtcttgt ttttctgatg aaaacaagat tttttattaa gctttttatt atttcacttc
    55801 gatttcacaa gagagacact ctgatttcac caaatagaaa taaaataggt taaataaatt
    55861 aaaaaataga agcgtaatta aataaggtta caagggggag tacttatgaa atggtataca
    55921 tggttgacta ttatattgat aatattgctt tctattccgc ttaaaattaa atttataaaa
    55981 tggtgggata gacgacaaaa aaatgataaa ggaggttatg atagatgata aaagtaggag
    56041 agttaaaatt ttcttatgga aaagataaac agatattgca tggacttaat tttgaagtaa
    56101 aagaaggaga aatctttgga ttccttggac ctaatggttc aggaaaatcc acaacacaga
    56161 aaatcttaaa tggtgtttta aaaggttaca atggtcacgt atcccttttt ggtaaggaag
    56221 ttaaagcata cacagaatct ttatatcaaa agattggtgt tttatttgaa tttccttatt
    56281 tatataccaa tttaagtgca attgataatt tagaatattt ttcgtctttt tatcctaaaa
    56341 atcaaaggag agatatatat gaactcttag atttgttaga gtttaagaaa gagtttataa
    56401 ataagcctgt atcatcctac tcaaaaggta tgaagcaacg tattagtatg gcaagagcat
    56461 tggtaagtaa tccaaagttg ttatttttag atgagcctac tagtggactt gacccatctg
    56521 gagctgtact atttcgtaag attattgaag aagaacgaaa aaaaggaaca actatctttt
    56581 tgactactca taatatgcta gatgcagatt taatgtgtaa caatgtggct tttattgcag
    56641 atggaaagat tatggtaata gataaaccca aaaatttaaa aatgaaaaat agcaataata
    56701 aagttgaagt ggaatttgta tataatggaa atcgagatat tgaaattgta gatatacaag
    56761 agttagaatc tggaataact tttaaatatg atgaaatctt aagtgtacat tcaaaagaac
    56821 ctacactaga agaagttttt attaaataca caggaaggat gttagtgtaa tgtactctaa
    56881 attttttacc ttagtaaaaa aagatttttg gatgatgatt tatggtaaat tttttgtgct
    56941 gacacttggc tctctcatac tttattcgtg ttatattaat tttggttatg taaagtttat
    57001 ggatgaagat agttatcaca ataatgtata tctatataat ccagagaata taagcatgag
    57061 tgcatctcca ttaattaatc aagtttcttc tataggtgaa ttaaaagcaa aacttgatga
    57121 agatacaagt gcagtttgta ttgatatgag ttccagtaag ccgaaagtaa ttttacatga
    57181 aagtattaaa ggagttgacg attatagagc tgactatgca ttgtcattat tatcatcaga
    57241 aaaagaccac aatagcaaaa tatcagacaa caatgcaata attcaaatta atgataagga
    57301 tacatctgag ataaaacaaa gaaaagacat gacttgtgag attttattct ttgaaattgt
    57361 agctattgga tttttaggga ttgcctcttt attatttaaa gaaaagcaga tgggagtaat
    57421 gcgtattact ggtattttac ctgtaaaaag aagtcttttt atattatcaa agatatttgc
    57481 ttttttgata tgtgacctat gttttacagt tcttttagta gtatttaata ttggattttt
    57541 tgaaggaata aaaatacttc cacaagtatt agttcaagct agtattttat caattattat
    57601 gtctttgatt ggaataggat gctcaatgtt attgagagat tttaagcaat tttctcttat
    57661 atatcttgtg gttgctgtat tcataactac acctatattt ctatctgcaa atacatcttt
    57721 tgaaatagat tggattaaat atcatccctt ctatcatgtt tatagtcagc ttaaaaatgc
    57781 gtttttcatg aatttttcaa aagaattagt ttattttgta gtgtgttctt tgacaataat
    57841 agttctattt ttacttgtaa aatatatttt taataaagag ttggttaggg agggataatc
    57901 ttgataggat tacgttacca gttaaagagt ataaaaaaag ataaaatgtg tataatttcc
    57961 ttctttttac cagttgttat ggcgatttta ataaatttta tgggaacaat tgatatatct
    58021 tcattaggag agatacgttt tggtatagtg aaggatacac ttcctgtaca aacagagcag
    58081 tggttaaagg agtatggtac agtaagtgta tatgacacaa aaatgcattt aatacgtgaa
    58141 attaaggata ttaaaacgga cacaattgga gttgttagag atggtaaagg gataaaaacg
    58201 gtactttatg gaaatgagat taatgctata aaaaatgtcg gaaatacatt gcaagatatt
    58261 tttaggataa agggagattt aaaagatgtt gaggttacaa tattgcctaa agatagtatt
    58321 ttaaaagagt ttcaaaatgt ttttattgct atgattttaa ttactgctat gtttatggga
    58381 tgtaccttta atgcaatgaa tattatatct gaaaaagaag atggaatatc atttataaat
    58441 gaagttttac cacagagtag aagtcagtat attatacaaa aagtattttt gggtctttta
    58501 tgtgggtgtt tatcttcaat gataactgta tttatatgct tggatatttc aatattagat
    58561 atgatagtga tgttgctact cgttgtattt tcagcattta tttcatcact tataggttta
    58621 tttgttggaa agttgtcaga aggccttatg gtaggaattg tttatattaa aatagtaatg
    58681 attttattta taggtgctcc tttgttaata tatcttttag ggattaatat aaatggtata
    58741 gggaatttat gttatataat gccatcagtt gctacattta atggtattat ggagattatt
    58801 aattgtaatg ccattgttat aaaagatatt gtaattttgg ctgtacattg tgtagcatgg
    58861 tttatgttgt atatgatttt agataaaaaa agaaagttat cttttagtag ataactcagt
    58921 ataattataa ttaggataat ttaataggtg atttaatata ttttgtatag tgtaatttat
    58981 gtagtgcaat ttgtatagtg taatttatgt agtgtaatag ttagttttat tttataatta
    59041 ttcaatataa tttagtaaga agttcagtac aattagagct acttagtgga aaacttaata
    59101 ggtacttaaa ctatataaat actttatttt cctccatttg tactataaat ttaatttata
    59161 atataaatgg aggtattatt atatgatttg ataaatttca tcattataac ttacaagtaa
    59221 aggtattatt atataaagtt aataaagata gtatcaaaat attatttgtt ataaagagag
    59281 gggctcaaaa taaagataaa cctatttttc aatgttgaaa tgataaaaat atatttgatt
    59341 tcatataaaa aagttttctt cataaattaa aaagtccatt ttgatacccc tcatttttaa
    59401 caagataaat ctagttaaac tatttaatta tattactcgg attcgtttga tttaaatatt
    59461 ccttgtttgt aaaactgttt tgagtagggg atagccaaag caaaaaataa gagtataatc
    59521 cctgctacga ttattaagtt attaatagat attacatctg ccattgggcc gaaaatcacc
    59581 ataccaagag gtataaaacc agtatatata ataataggta agctaagaat tctaccttgc
    59641 atttgtggtg ctacatgttc ttgtaacata gtcatagctg atgcttgaat aattggaatt
    59701 gaaaaattcg taaaaaacat aaatataaaa aattgccaag cttgtgttac aagacctaga
    59761 ataattgaaa aaatagcata agataatatg gatagaaaaa aggtcttatt tctattttta
    59821 aagccaccaa atacccctat aatcaatcca cctaaaacca tacctaaaaa tccaatcatt
    59881 tcaataatag acaggtatat atagcccttg ccaaatgttc gttcaataag aagtcctata
    59941 agaaatcctg atggaacaca aagaagcatg aatgctccat aagtgaaaag cacttttcta
    60001 atgaatttat ctgaaaaaca gtactttatc cctgatacca tatctgcgaa aaatgagact
    60061 ttttcagttt gttttggtat atcatgttta ggaattttga tgaatatcaa caatacaata
    60121 gcaataattg cagtaaatac atcgattaaa agaacttgat ttattttatt accaaatcca
    60181 agtattgcag cagccacaat aggaactata aattgtacta tagactgtag gctaccattt
    60241 ataccattaa aacgcattaa atgttcttct ggtacaagtt gaggaatcat tgcatttaca
    60301 gcaggtgttt gtacaccact tcctagtgaa cgtataacag atattactga tatggctatt
    60361 aattcacttg ttgttccatt tgatgacatc ataaatagaa ttagaactaa tgtagatata
    60421 gcaattattg catctgatac aattatgagt acctttcgat tgtagtagtc tgcccataca
    60481 ccagcaaaaa aagaaattat catttgaggt ataaaggaac atacagttaa aactgttacc
    60541 catacacctg atgatgtttg aagtgtgaca taccatatca ttgccatctg tacaagtgat
    60601 gagccaaata aggaaatata ttggcttgct aaaaaaagaa tgactttttt cttccatgta
    60661 gaattagtat ttttgttttc catcctattc ctcctttgat ttgtgtgttg aatttttctt
    60721 gctcttaagg ataacataca aatcaaagtc tggagttaga gtggagggct aggaaaattg
    60781 ttttttcata atttgaagat gagatttcat ggtttttgca ttatgttcag cttctttcaa
    60841 tgaagttatc agtttgtcac acactgagat aatttcgtca tcatcttttg gagtgtcaag
    60901 gacagtttta atattggtat taggattaga agataaagca tttaacattc gtaaaatagc
    60961 tgaaagagaa tagtttgcac aacgaagtgc gctgataatt ttaagtcgct ttatatcttc
    61021 gtcagtgtat acgcgataac cattttgttt tctttttact tttaacaaac cattcatttc
    61081 ccaatttctc aaagtatcta ttgtaacatt taagtgttct gcagtttctt ttctagttaa
    61141 aaagagttta tctgtttctg gagtatttcc atttaaaatc tgtgaaacaa tagtaagtgc
    61201 ttcctcggca ttagattttt ctatttctag ttgttgcaag tattgattta taagacaaat
    61261 agccttgtca aaatctctat ttgctgatgt tctgacaatc tcaagggcat cttttcttaa
    61321 tcctctttgt acaacttcaa ttttcaaggc tgtacgagca aatctaaatt gttctatatg
    61381 aaaatcatta aagatacgat aaccgttggt attacgctta ggtttagata ttaagtttag
    61441 ttcttcatat aaacgtactg tattaggatg tacaccaatt aaatctgcta cctctgatgt
    61501 cgtataagtg ttcattagta ttcaactcct tttagatata tagtactagt cataataaca
    61561 ccaaacaaaa gtctggtgtt atagtggagg gtttggttat ctaagagtaa ttagtttatc
    61621 taagagtgat taatctatta tagaagtaag tatatatttt tcaataaact cccatctatc
    61681 acgaggttta aacactattt tagatggtat ggctattact aagtcatatt ttgggataca
    61741 acaaatagca tttcctccag ttcccattgc tgaataccac aaaactccat cttttttaca
    61801 tagcaaccac agataaccat attcattgcc attcatttta actgattctt caatccacga
    61861 tttagatata atacgtttat tattccaaat accatcattt aaatacagaa aaccaaatcg
    61921 caacatatct ctagcactca atgtaagtcc ccagccacct gtagaattgc cctgtctatc
    61981 ttttgcccaa cccttaagat tttttccaaa tagatcttca aaaccaaaat ctttcatatc
    62041 ataatctggt ataactttca taccaatagg tttaaataag tactcattag caaattcacg
    62101 agcagatttt ccagtagttt ttgttataat agctgaaagt aagtgcgttc cagctgaaga
    62161 atatttgaaa gcaccaattt cacctttttg accaagctga ttcaaggtat attttatcca
    62221 atctttttgt ttgcaaagtc tctctaagga ttcattcata tttttaaaag gatatggcgc
    62281 agtcattgta agcaggtgac gtatagttac tgctttttta catatttcag aaggactaca
    62341 agtatattca gggaaaaaat caacaacctt ttggtcaaca tcttttatat atcctttatc
    62401 tattgcaatg cctattaagg ctgatataat actttttgta accgatgcaa catgaaaagt
    62461 atcatcagca ttatatccat tatagtactt ttcaaagaca acttttcctt ttctcacaat
    62521 aattattcca tttatattgc tatattgtga gattattaaa ggttcaagtt gtgaaagctt
    62581 ttctgaactc atgccaacac ttgaagggtc tactttttca aaaattaagt ctaagcaatt
    62641 gtttttattc attaaagata cctccctgtt atagattttg gattttcttt tagagctttt
    62701 ggagaacctt ctgcaattat ataacctcct ttgtcgccac cttctggacc aagttcaaca
    62761 atccagtcac aaacatttaa tacatctata tcatgttcaa caacaactac agaatttcca
    62821 tttaatacta gttcatctaa taattgaatc agcttagcag tatcatacaa acttagacca
    62881 gtagttggct catccaatac atagaggata ttaccatttc gttttttacc aatttctttt
    62941 gcaagcttga ctctttggga ttcaccacca cttaaagtcg gtgtaggttg acctaatttt
    63001 atatacccca tacctatttg ttctaataca tttaagtgtg aaacaatatt tggaatatct
    63061 ttgaagaaat gtacagcttc agagacactc atatttaaaa tatcatgtat atttttattt
    63121 ttgtatttaa tagataaaga ttcatcatta aatctttttc cctgacattc actgcatata
    63181 tggtctatac ttagattatt tcccaaccag accttttcat atccactacc actacattta
    63241 ggacaagccc ctttggagtt aaatgaaaaa tgaccagctg taaatcctcg ttctaacgat
    63301 tctggctgac ctgcaaatag tgtacgtatc ttatcccata taccaacata agaagcagga
    63361 gttgagttca tatttttgcc tataggagat tgagatatct ctgaatatcc agaaatatgt
    63421 ttagtaccaa tcagtctatc tgcaatagtt tcaacaactg cataatcgtc ttcctcacct
    63481 atttcactct ctgaagaatt tatttgatta ttgatagtat ggttattaaa ttggcttcta
    63541 agaagaggaa gtagggtgtc tgatataagt gaactcttgc cactacctga ttttccagat
    63601 attccaacca tagcacccaa aggtaatgat acagttacat ttttaagatt atttgtattt
    63661 gcattttgaa tagttataca aggtatttta tcagtatgtg taatgttttt attgcttgtt
    63721 ctattaggca ttggatattt tccagataga tactgtccag tgagtgattt gtcggattga
    63781 agtagacctt ctaaatctcc ttgatatact aattgaccac cttcaacacc agcttttgga
    63841 ccaatatcaa taatatgttc tgccatttta attgtgtttt tatcatgctc aactactatg
    63901 accgtattgc ctaaatcttt aagtttcttt attgatttta agatgtctat tttttctgat
    63961 tcatgcaatc cagctgttgg ttcatcaagc acataaatta atgagtccat ctctgaatcg
    64021 agatgtgaat ttaaaaatag tctttgcatt tccccaccac tcaaagttga catttctcgg
    64081 tataaggata gatgacctaa tcgtgattct attaaatgtt gagttttgga taaaatatca
    64141 tttacaagat ttgtaccaaa tgtgtttaaa ttaccttgat ttattaagag tttaaggaaa
    64201 tcatcaattt ctaaaatcgt catttttcca agttctccaa tatgttttcc gtttaaaagt
    64261 actctacgtg cttcttcacc aattttaaaa ccattgcaat ctgaacaggc agtcatagta
    64321 taaactccat ttaaatcttc gccccttttg taacgattgt gaaatacctt tgataaagag
    64381 gaactttttt taccattttc ataatgacca tagagtactt catttcttac ttcttcaggt
    64441 agttgtaaga attgtatgtc taaataatca ttgaattttt tagaaaatac tctcaaatat
    64501 ccaggtgtaa tctttgctct atctaagact tgttttaggg tatcatgctt atcaaggact
    64561 aatttttcaa tattaatctc aaaatatgca ccatgtccag agcacttcat acacattcca
    64621 tctgaagagt tgtatgagaa ataacttggt cttaatcgtt cttcctcata tccacaatta
    64681 ttgcaaatta gattatagct tacaggagta tcacacatag tacacattat ttgaccttcc
    64741 actgaataca atagtgtaag catgttgaga atattggttc ttgtgcctac tgttgaacga
    64801 ggattgctct ggcgaataat attttgttga acagctatag tgggagctaa accttgaata
    64861 ttatcaaact tatcttcact atcaatacct gcaagtatac ctaaagattg cagatattgt
    64921 ttacgccctt cctcaaatac aatgtcaaac ataaggctag acttacctga gccactgaca
    64981 cctgttgcaa caacaagctt atctttgggg atagaaacat ctatattttt aagattgtgt
    65041 atctttgctc cattaatttt tatttctttc attgcttcac ctcattttat caattttatc
    65101 aaaccgtaaa atagggatat attaatcctt aatacaagaa tagattaggt gaggttatta
    65161 aaaaggttct ataggtaaat atatatcgac caagtgtcta ttttgagggt gattatatgg
    65221 gttgttaaga tatacttcaa aagggaagga ggcacgtaat ttataattac cattagatag
    65281 ccattcatta tacataaaat cccacgcatc actatattca gattttgaaa tatcaaaatg
    65341 tcccacaaga tattttccag aaggtattat catattgcca agattgtcat tatctttgac
    65401 aacaatgtta tctggaatgg tcatgcaaat actagtcctc aattgatgct cctgtgtaaa
    65461 ttgtggatta tcatgataaa tggctaatat ttgtgtgttt tcaagtccaa gtagattctg
    65521 ttcacttgta tatgtgaata acctttctaa aagttttggg aaagtactag ttagattacc
    65581 atatgaacca gtatatctag tgtaaatagt attcatatta tcaagttcta gaatttcaat
    65641 ttcaccttta acctctctag tattcttaca tttaggtttt gatatgcttc tattgtactg
    65701 agaaatttta tatggttctt tgcaattctt gctataatta tttctatatt ttatagggct
    65761 tattttatag taatttttaa atgttctcga gaaaactgct gaatcagtaa agccaaagta
    65821 gtgtgcaata tcagtaacag tcatgtcagt tcgatgaata agaaaagcag ttgcgttttc
    65881 tagtttcaat ctatttacat actgtgccaa agtttctttt tctattgctt tgaagattct
    65941 atgaaaatga aattttgaaa aacctgctat attagaaagt tcgtcaaggg aaagaggttc
    66001 atgtaaatgt tcttctatat agtcttgcac tttgcaaata cgatttagat attctttttt
    66061 acttttagcg tttgtcatat agccctcctt attgtgtaaa atgtgtatta attaatctat
    66121 agtaatatag tagtaaaatt ttatgttttt gtaatttatg ataaataaga tagcgtccaa
    66181 tttttgataa ttaataaaag atatttataa ctaaaaaaga tataagatgc cttatat
//
LOCUS       DAGSHT010000003        63308 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGSHT010000003 DAGSHT010000000
VERSION     DAGSHT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.004"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(72..711)
                     /locus_tag="MJB72_000135"
                     /pseudo
     CDS             complement(72..711)
                     /locus_tag="MJB72_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1121..1297)
                     /locus_tag="MJB72_000136"
     CDS             complement(1121..1297)
                     /locus_tag="MJB72_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261316.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1351..1447)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2243..2644
                     /locus_tag="MJB72_000137"
     CDS             2243..2644
                     /locus_tag="MJB72_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBY8261317.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            2641..3708
                     /gene="rsgA"
                     /locus_tag="MJB72_000138"
     CDS             2641..3708
                     /gene="rsgA"
                     /locus_tag="MJB72_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBY8261318.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYYDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKAEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            4244..4930
                     /locus_tag="MJB72_000139"
     CDS             4244..4930
                     /locus_tag="MJB72_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261319.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDSNKKEKVS
                     EDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTD
                     EQKKHINVLI"
     gene            5108..5998
                     /locus_tag="MJB72_000140"
     CDS             5108..5998
                     /locus_tag="MJB72_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261320.1"
                     /translation="MKKNILTTVITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGDNYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(6400..7356)
                     /locus_tag="MJB72_000141"
     CDS             complement(6400..7356)
                     /locus_tag="MJB72_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8261321.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKKMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            7772..10816
                     /locus_tag="MJB72_000142"
     CDS             7772..10816
                     /locus_tag="MJB72_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBY8261322.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTASHNGISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDNSGNTEVSEVKATTVYIKELTAPMGFKVDKT
                     IYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYTKDNLPAEATRTWVTKTIAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTCEETKSPTGYLLDGAYMQANGSEEQIKGLYLIQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFAITENGKIVDLTDEAHSV
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVITDDNGQFSTSSDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSESNKDFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEVGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETSTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRCKMKKS"
     gene            11052..11378
                     /locus_tag="MJB72_000143"
     CDS             11052..11378
                     /locus_tag="MJB72_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY8261323.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFAPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            11393..11776
                     /locus_tag="MJB72_000144"
     CDS             11393..11776
                     /locus_tag="MJB72_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY8261324.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQAVNPQNHAPQGQPKK"
     gene            11874..12359
                     /locus_tag="MJB72_000145"
     CDS             11874..12359
                     /locus_tag="MJB72_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261325.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            12476..13870
                     /locus_tag="MJB72_000146"
     CDS             12476..13870
                     /locus_tag="MJB72_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8261326.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNLKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVYALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            14072..15271
                     /locus_tag="MJB72_000147"
     CDS             14072..15271
                     /locus_tag="MJB72_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY8261327.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLVAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCEHEECISKFRSFKSYGSGELVKHNETDKYD
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHHQIIKQQTATVEE
                     MVANVKE"
     gene            15278..15412
                     /locus_tag="MJB72_000148"
     CDS             15278..15412
                     /locus_tag="MJB72_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBY8261328.1"
                     /translation="MWELLKDLLLVSFGIGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            15413..15634
                     /locus_tag="MJB72_000149"
     CDS             15413..15634
                     /locus_tag="MJB72_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261329.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            15718..16248
                     /locus_tag="MJB72_000150"
     CDS             15718..16248
                     /locus_tag="MJB72_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8261330.1"
                     /translation="MCKILLVRPSKQYADQVMSYKEEMSQNGDSFDGCAGLENVQSFE
                     EWIDFEARLKAKYKDGYVPSEVFLAIRKKDNYVIGMIDFRHPLSDFLFNFGGNIGYSV
                     RPSERQKGYASEMLRLILPICHEFGESKVLLTCDKKNEASQKTIVRNGGVLEKEIPDT
                     VGLSESGIIQRYWISI"
     gene            16323..16865
                     /locus_tag="MJB72_000151"
     CDS             16323..16865
                     /locus_tag="MJB72_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY8261331.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNHCGGCGNGNQSCKIARC
                     SLEHGMIEYCYECKQYPCEKYQHIDDYDIFITSRRQKADLERAKIIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EMFQKIADRRNIKLKLIKKK"
     gene            16969..17265
                     /locus_tag="MJB72_000152"
     CDS             16969..17265
                     /locus_tag="MJB72_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY8261332.1"
                     /translation="MNDIFKDMQAKVGCENISDLPSYKRKVWHEMKQLNFADYEEKQL
                     EDFSNYVFGMSCQTLKDVMKQQKGREEQCRKQGCWWKRAEQLAKKQHHTGSTCR"
     gene            17184..17687
                     /locus_tag="MJB72_000153"
     CDS             17184..17687
                     /locus_tag="MJB72_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY8261333.1"
                     /translation="MQETRVLVETSGTTGEETTSYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPFADEVHEHTSVYQLNELDFMYRQLSSDMQEEYTALLTVYENLEALYICR
                     NVIAVYPDCKSMIDVARQKLMNDPTFKHLSKDCQEYYFDFEAYASHLLENRRYLVTEH
                     AVFELPE"
     gene            17705..18208
                     /locus_tag="MJB72_000154"
     CDS             17705..18208
                     /locus_tag="MJB72_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY8261334.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQC
                     EEYAIHDTDNFPIEIGEYVSIEELNEMYEMIEELPDYIVECLDEFIGHYGTLEEVMEH
                     KDNIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            18318..18707
                     /locus_tag="MJB72_000155"
     CDS             18318..18707
                     /locus_tag="MJB72_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY8261335.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFFI
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLDKQILNETITAVRSVNYVPD"
     gene            18694..21144
                     /locus_tag="MJB72_000156"
     CDS             18694..21144
                     /locus_tag="MJB72_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8261336.1"
                     /translation="MYPIKYIDNNLVWNKDNEVYAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLREVAVRKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEELLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIDDFGYLMEHLYGRDGIAYEDYEYQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFAVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENMGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRREDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            21144..23279
                     /locus_tag="MJB72_000157"
     CDS             21144..23279
                     /locus_tag="MJB72_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY8261337.1"
                     /translation="MKERIQSAFTKKKMFRFLKITVFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALYAVINFVVVFILSASFIACAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SANPEDEDGKTREEVVKTEIENNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVDGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDMPTQTAYAVYSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPATAPT
                     SKTSKPVKINSAKKRPLSSGFSDVKAPQSAQPVHRQNVEKVPVSEKTRQNFKAERTTK
                     AHIVQQPKKITETTEKNRNAVFTKGQKKK"
     gene            23276..24283
                     /locus_tag="MJB72_000158"
     CDS             23276..24283
                     /locus_tag="MJB72_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBY8261338.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVRYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTADLQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSNVTHVGIYVGNNQMYHAGDPIGYADLNSSYWKQHLIGAGRVK
                     Q"
     gene            24299..25201
                     /locus_tag="MJB72_000159"
     CDS             24299..25201
                     /locus_tag="MJB72_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY8261339.1"
                     /translation="MFKKNKKPTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISD
                     YLTKELQDLNVDTIRTDIPTSSAVTDVIVWSIEQLGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDADGDMVIIQNPTLAPAIEKSEYEPETLETDASVDADTVNDATAFLE
                     TFFNLYPTATEKELAYYVARNVLEPIGKDYLYSELVNPIFIKDGDNVKVKVAVKFLDN
                     QTKATLVSQYELVLHKDSNWKIVG"
     gene            25304..26119
                     /locus_tag="MJB72_000160"
     CDS             25304..26119
                     /locus_tag="MJB72_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBY8261340.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALDCSNDTIIEQLDKKSIEIMDNIQKEQEKLKKIE
                     IAKKELLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            26200..26676
                     /gene="tsaA"
                     /locus_tag="MJB72_000161"
     CDS             26200..26676
                     /gene="tsaA"
                     /locus_tag="MJB72_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBY8261341.1"
                     /translation="MTTYKVNSIGTIYNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTDFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSVVEIINIDYMNGMI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPNVPDWCSHWPHSLEESASFAWENEFNF"
     gene            26690..27241
                     /locus_tag="MJB72_000162"
     CDS             26690..27241
                     /locus_tag="MJB72_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8261342.1"
                     /translation="MSLQDYIIEKPVIDTEHLILRTMDKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPEVLFSKPEKATKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKQYQGNGLMSEALKSIIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITS"
     gene            27508..28419
                     /locus_tag="MJB72_000163"
     CDS             27508..28419
                     /locus_tag="MJB72_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8261343.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKK
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWVIPV"
     gene            28497..28931
                     /locus_tag="MJB72_000164"
     CDS             28497..28931
                     /locus_tag="MJB72_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY8261344.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            29399..30139
                     /locus_tag="MJB72_000165"
                     /pseudo
     CDS             29399..30139
                     /locus_tag="MJB72_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            30090..30701
                     /locus_tag="MJB72_000166"
                     /pseudo
     CDS             30090..30701
                     /locus_tag="MJB72_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            30914..31771
                     /locus_tag="MJB72_000167"
     CDS             30914..31771
                     /locus_tag="MJB72_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8261345.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYIMEDMLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVGSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHAPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            31849..32430
                     /locus_tag="MJB72_000168"
     CDS             31849..32430
                     /locus_tag="MJB72_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8261346.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDAIMLSLTGLI
                     TSPILSQIDLSLMDYLQRKLSPNIFENHMHNDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            32534..33379
                     /locus_tag="MJB72_000169"
     CDS             32534..33379
                     /locus_tag="MJB72_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261347.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            33376..34059
                     /locus_tag="MJB72_000170"
     CDS             33376..34059
                     /locus_tag="MJB72_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8261348.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSDSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            34056..34754
                     /locus_tag="MJB72_000171"
     CDS             34056..34754
                     /locus_tag="MJB72_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8261349.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            34766..35677
                     /locus_tag="MJB72_000172"
     CDS             34766..35677
                     /locus_tag="MJB72_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY8261350.1"
                     /translation="MEKGRLLQNKNINKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSEQYNTLSAPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            35729..35869
                     /locus_tag="MJB72_000173"
     CDS             35729..35869
                     /locus_tag="MJB72_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261351.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNSPKDSEDDK
                     MY"
     gene            36159..36785
                     /locus_tag="MJB72_000174"
     CDS             36159..36785
                     /locus_tag="MJB72_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8261352.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNVLYQKCLVNGPISYIEDIFNEMIKKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            36907..37623
                     /locus_tag="MJB72_000175"
     CDS             36907..37623
                     /locus_tag="MJB72_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY8261353.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGSGDYLNFIAIILQKK"
     gene            37787..37978
                     /locus_tag="MJB72_000176"
     CDS             37787..37978
                     /locus_tag="MJB72_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBY8261354.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            38002..38232
                     /locus_tag="MJB72_000177"
     CDS             38002..38232
                     /locus_tag="MJB72_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261355.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            38499..38918
                     /locus_tag="MJB72_000178"
     CDS             38499..38918
                     /locus_tag="MJB72_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8261356.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELLDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            38921..39175
                     /locus_tag="MJB72_000179"
     CDS             38921..39175
                     /locus_tag="MJB72_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8261357.1"
                     /translation="MKKKYKHPSYYLISLAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            39550..39753
                     /locus_tag="MJB72_000180"
     CDS             39550..39753
                     /locus_tag="MJB72_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBY8261358.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            39831..41021
                     /locus_tag="MJB72_000181"
     CDS             39831..41021
                     /locus_tag="MJB72_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY8261359.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     KLAS"
     gene            41776..42771
                     /locus_tag="MJB72_000182"
     CDS             41776..42771
                     /locus_tag="MJB72_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8261360.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            42749..43612
                     /locus_tag="MJB72_000183"
     CDS             42749..43612
                     /locus_tag="MJB72_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBY8261361.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGVDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            43609..44403
                     /locus_tag="MJB72_000184"
     CDS             43609..44403
                     /locus_tag="MJB72_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBY8261362.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            44688..46067
                     /locus_tag="MJB72_000185"
     CDS             44688..46067
                     /locus_tag="MJB72_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8261363.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            46515..47228
                     /locus_tag="MJB72_000186"
     CDS             46515..47228
                     /locus_tag="MJB72_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8261364.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(47500..49038)
                     /locus_tag="MJB72_000187"
     CDS             complement(47500..49038)
                     /locus_tag="MJB72_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY8261365.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            49920..50375
                     /locus_tag="MJB72_000188"
     CDS             49920..50375
                     /locus_tag="MJB72_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBY8261366.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            50424..51008
                     /locus_tag="MJB72_000189"
     CDS             50424..51008
                     /locus_tag="MJB72_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBY8261367.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(51013..51336)
                     /locus_tag="MJB72_000190"
     CDS             complement(51013..51336)
                     /locus_tag="MJB72_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261368.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            51488..52006
                     /locus_tag="MJB72_000191"
     CDS             51488..52006
                     /locus_tag="MJB72_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY8261369.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(52182..52850)
                     /locus_tag="MJB72_000192"
     CDS             complement(52182..52850)
                     /locus_tag="MJB72_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261370.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            52965..54380
                     /locus_tag="MJB72_000193"
     CDS             52965..54380
                     /locus_tag="MJB72_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY8261371.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            54603..55061
                     /locus_tag="MJB72_000194"
     CDS             54603..55061
                     /locus_tag="MJB72_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261372.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            55058..55642
                     /gene="clpP"
                     /locus_tag="MJB72_000195"
     CDS             55058..55642
                     /gene="clpP"
                     /locus_tag="MJB72_000195"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBY8261373.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            55954..57030
                     /locus_tag="MJB72_000196"
     CDS             55954..57030
                     /locus_tag="MJB72_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY8261374.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            57097..59172
                     /gene="bclA3"
                     /locus_tag="MJB72_000197"
     CDS             57097..59172
                     /gene="bclA3"
                     /locus_tag="MJB72_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBY8261375.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            59341..60102
                     /locus_tag="MJB72_000198"
     CDS             59341..60102
                     /locus_tag="MJB72_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBY8261376.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            60411..60689
                     /locus_tag="MJB72_000199"
     CDS             60411..60689
                     /locus_tag="MJB72_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261377.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            60982..61557
                     /locus_tag="MJB72_000200"
     CDS             60982..61557
                     /locus_tag="MJB72_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBY8261378.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPNRLSTETLEQFHTNDDNLANQYAMNVELLHRNG
                     AEVGIPEYKIVGQEIDINDLTQVRIVSKYPSQLKVSALLREKLGLSRKAFDEMLDCGT
                     IKSISGVDLRRSKLNYEIVLEVGKQKQKSSN"
     gene            61996..62706
                     /locus_tag="MJB72_000201"
     CDS             61996..62706
                     /locus_tag="MJB72_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8261379.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            62700..>63308
                     /locus_tag="MJB72_000202"
     CDS             62700..>63308
                     /locus_tag="MJB72_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8261380.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILL"
BASE COUNT        22330 a         9270 c        12117 g        19591 t
ORIGIN      
        1 atttaaaagg tttttgtata ttgactaaaa tttattttta ttatttataa ttcttaataa
       61 tacatatgta cctactgatt ccatattaaa ctgtataatt tttgttcatc tttttttata
      121 attaaaacta cgtagttctc ataatttctc ttatatatat acttatcatc ttttttaact
      181 tctaacttat attttttaaa atcttcttca attttatttt tattactttt attaatgtta
      241 acagaatctc ttttaaattt atcaatatat ttttctattt cattaatatt atctttgctt
      301 acaactatac caccttttaa attaaaaact tttttgaaat tttttttcag aaaatccttg
      361 aataaaataa ctttgtccat ctccattaat actatcttga gtatcaaata cttcaatttc
      421 tttgttaggt attggtatat caaaattcca agattccttt agcattgaaa tattagctgt
      481 ttttcctaaa aaaagattat ataggatact tcctaataac aaaacaccta ttaatagtag
      541 tgtgattaat acaatttttc tctttttcat ttttcgttca caaccttttg aaaaaacatg
      601 atttaattca tcatttgaag gcatgtcttc aagaataatt tttgttgtga taggaatgct
      661 ttcttgaaac atatcatttg taatatcaaa atcttttctt tgattactca atatccattt
      721 cttctaatat ttcttttgat tttttctagt acattgaata ctcttatata cattctcctc
      781 ttttaaaatt aaaagttata aaagaataag ttatataaaa aattattata ttttttaata
      841 tagcttattt gcaaatttat aattttgtta atctaatcct tcaaactaaa aaatcattta
      901 tgtttacaga atttcatttt aagagatgtt tattttcaca tagataagag aatttaagtt
      961 ctcatattta ttatttgata caaatttaca tctttattat atgtatttta aataaaaaat
     1021 aaaattatga atactagaaa tctatcttaa ttagctttct aacataaggc tatttaaagt
     1081 agataaaact aaaaagggtt ctctagaaaa ccaaatctat ttaattatac ttacttacta
     1141 ataccatcac ttgatgcaaa caagattcta ccacattttc tccgtactct agtataaaat
     1201 catctagttg tgtatctata aaatcacaat aatcatatga actaccaata taagcagtca
     1261 acatatgtgt acatctatcc attttatctc taaacatata tattattatc cccctaaaaa
     1321 actttcataa aaacaaaaag aatatctctt tttggcaact ggctgacata actcatagtt
     1381 tattttataa gcgttagtcc ctatagcttt gcgtcactaa atttctctag ttttgccgat
     1441 ttagttttat tctacaatta caataataca atagaattgc ttattattca atatacttat
     1501 ttgaaaatta aataaattta caaatatgaa cgttatttta tatcaaaaaa atagactatg
     1561 catgtagaat atatggaaaa acttgattgt aggttttgta tgaaaagaca gttgttatta
     1621 atttaagtga gataaattga gcaataggca taaaatgaga aaacaaataa tagcagttaa
     1681 tttttattat taatactagg tgaacattaa attttaaaat accactagat tttatagaat
     1741 aagaaaatga gacaaacata aatatatgaa tatattaaat tatacttgaa catacttata
     1801 ttttatgata aaatttcaat aataattatt agtgaaggag tgtcttgcct gatgtcagag
     1861 ttatatataa tggtaaccaa caaataaata gttggagacg catatgggtt ctgtttaatc
     1921 agaattttta agtgtatttt tgatacattt gattgttgtg tcttactctg cctttataga
     1981 taactaacat tagacaacaa ataataatat tacatattac tttgttaaat tgtggtgtag
     2041 tgggatttaa tctgctacac ttttattaat tttacacagt attattttga atataattat
     2101 gttgaaatag aagatattaa attttctagt ccacaggggt tttctgtttt tgtgtaggta
     2161 aatatgtata ccacataaaa aggcagatat aaatacctgt ggactttttt tgtagaaaat
     2221 acaagatacg gaggaaataa aaatgaatta taaaaataga aaacgagctt attatcagga
     2281 aaattcctgt atggataact ttatttgcaa aaattgtggt tgccttgtag ttcctgaagg
     2341 agctggtaca caacatagaa atcattgccc acattgttta catagtcttc atgtagatat
     2401 cattcctggt gaccgtgctg cagattgtga tgctaatatg gaacctattg gagtgtgggt
     2461 gagaaaaaat ggtgagtggg caattattca tcgttgtaca cgctgtggac atcttagttc
     2521 taatcgtatt gcagctgatg ataattcagt gaaattaatg tcaattgcat taaaaccact
     2581 ggcacagccc cctttcccac tagaaagaat cattgaaaag ttggaaaagg aggaaacaga
     2641 atgaatggta tgaaacaaat caatttacaa gactatggtc taaataggaa ttttattact
     2701 caaagtatgg aatattatga tttatacctt gctcggatta tctcgcagga aaagaacttt
     2761 tatcaagctg tatgtgtaaa tggtgtactg tttgcagagg tttcaggaaa gctgcgtttt
     2821 gaggctacta tacaatctga ttttcccaca gttggtgatt tcgttatgtt ggatcgttgc
     2881 aataataaaa atggtaatgc tattattcat catgtgcttc atagaaaaag cttgtttgtg
     2941 cgtaaggctg ctggaaaggt aaatgaggag caggtagttg cagctaatat tgacattgtt
     3001 tttttgtgca tgtcactgaa taaggatttt aacattcata gattggagag gtatctgtct
     3061 ttaacatggg aaagtggagc tatcccagta gtagtactta ctaaatcaga cttatgtgac
     3121 aatgtgcatc gtaaattaca agtagttaat tctatagcct tgggtgtaga tgtagttgtt
     3181 acctcatcta tagagccaga tggttataaa cagatttcta agtatattaa accaggctgt
     3241 actgttgcct tacttggttc ttcaggagtt ggaaaatctt cattaattaa ttgtctgatg
     3301 ggtggagaat ttttaaatac aaaggaaatt cgcatagatg atgataaagg aagacatgcc
     3361 acgactagac gagagctatt tttaatttca ggtggtggta ttgttattga cacaccaggt
     3421 atgagagagt tgggaatgtg ggatgtttca gaaggtttag ataaaagttt tgcagatata
     3481 gaagcttatt tcggaaaatg tcattttcgt aattgtactc acacctcaga accaggctgt
     3541 gctatctggg aagctattgc tcatcaagaa ctttcgccta aaagatggga gtcctatcgt
     3601 aggcttaaag ctgaagcagt atttgcagac aataaaagcg aatacttagc aaaaaaagaa
     3661 aagaaattta aaaatatctc aaaaataaat aagacgatta gaagataagt gtagtttttt
     3721 tgttcattct atgagtctat atactattaa tatattaaat ttgccatcaa tttgttactt
     3781 taatgtgatt ttgatggcaa atttggtttg tggtattatt taaagcaatt tattaatttt
     3841 tttaataaag tttgattttt tatatgatga tatatacatg tgataatgta tacatatttg
     3901 ttacgatagg ctcctttata aagaggtctt atgtaagata agaaaaggca tatttatact
     3961 aatacataat agagtatatg cttatgtatg atagagatgt ttcttaagag caaaactgca
     4021 atattgctaa tttataaagt aaaaatatat gaattataaa atacaaataa tatatttata
     4081 aaaattattg ttttaattta taataaaaat tttttgttga aaataatgct cgaacctata
     4141 aaactattaa ctaatatttg taaatgtata cttttgatga taaaatataa ttaagttaag
     4201 ttggttttta taaacataaa tttattttaa ggaggtgaaa taaatgaaaa tatctagtca
     4261 atatagaagt caatattcat ttagatatga aagtaatata aataatacaa gaataaatga
     4321 aagtatggtt aagaaaaatg aaactgtagg aaaagacact tatttatcta atattatgaa
     4381 acaaaagcaa gaacttaatg atagaattag agatttaaaa tatagacaag aggtttatac
     4441 taaaaaaata aatgacgcaa ttaagaactt atgtaaatca gaaataagag aaacaactaa
     4501 taacttttct aatatagaaa taggtattaa aaatagcatt atagaagata aaaataaaag
     4561 tacaatgtta gatgaaaatt caacttatct aaatacaaat gatgaaaaag aatctttaat
     4621 cactaaagag tctaatgaaa aaattgaaga agaaatatta aatgatgaaa aattagaaga
     4681 gttagaacaa aaaaaggatt ctaataaaaa agagaaagta tctgaggact tatctttagt
     4741 aggtaaaact cgtgaagagc ttgaaaatat gcttaaaaat tttataaatt taacacaaga
     4801 agaaataatg aaacttgagt cgagaataga aaagttagat aaaaatgctg aagaatacaa
     4861 acaaaattca aagactaata tatttgataa aacagatgaa caaaaaaaac atataaatgt
     4921 actgatttaa acaatatgtt ttaaaaaaat aagtttatta tataatgaaa atataaaggt
     4981 acaatttcaa tacacttcta ttaaaccaat aatttatgaa tcagacttaa tttcaattta
     5041 ctaataacca tatatttatg agttaataat tttatgtttt aaaaagaatt aaataagggg
     5101 gagtagtatg aagaagaata tattaactac tgtgattacc atgattatta tattgatgat
     5161 gagtatgacg gtaatggcag agccatatca agaagatgaa acatctagct tgaaaaaaca
     5221 gagtgcaaat gaacgtaatc agactaatat aggcacaatg gatggcagag tgcaaacaaa
     5281 tgcaataagt aaaggtgata attatgaccc taaatatcct cttaaaggaa tgttaacaca
     5341 acttggtctt aatacaacag gtaaagaggg atgggattta ctctatggag gaaatcccaa
     5401 tggagttgag cttcgagcag atggtaaata ttattatata aataagggta tggaagggct
     5461 aggattttat gcggcagcat tgtcaggtgg ctcatatcag agaataggct ttggaaaact
     5521 tgaccctata gaagtaatag ctgatggaaa ttggatttct tacaaacaag ctcaagatac
     5581 acttacagta ataaggaatt ttttaaatag ttttgactgg agaaatgcat ctgagatgga
     5641 aagagctaat agagctgcaa aattggtaac cgaagcaaaa tatgcagact ctaaatattg
     5701 taacatagtc tatggtaatc tggttgataa acgtggtgta tgtggaagtt ttgcaagtag
     5761 ctttcattta ttaacaaggt taatgggaat ggattcacta tctatattaa atccatcatt
     5821 aaatcatgct tggaactata tacaaattga tgggaaatgg tataggtttg atgggtctga
     5881 aatatcagct tttggtggat cattagactt tgattaccgt aaattaaagg atgcaacaag
     5941 agagatgact acttactatg atgcaaaggc attgtctata ttaggattta atcaataaaa
     6001 atagggggag gcttcttata gagccttttt ttattgattt gaaatttaat aaggaatttt
     6061 atatgaaaat ttaatcaata aaagcaaaaa aggctcttta taaagccttt ctaagtgttt
     6121 ttttaagtaa aattttttat atcaacatca gttttaatca aaacatataa cattttggac
     6181 ttacttttaa aaataaaatt attaaaatat aatattaatc atgttaataa tgtttttagt
     6241 ttattatatt ctataatagt ggtcatgttg actattgtag ttttattaag aatagttggt
     6301 ttatataaat atatgcaaag atagcttgtt gtttacttta cttattagta tataggacaa
     6361 gatactctaa taaatttgcc tattttaata tcttttgtac taaaaccaac taaagtttcc
     6421 tttattattt cgttttgaat aatacaaagc ataatccgat tgtttgtata aagactggaa
     6481 agtatcagag ttattttcta atttagatat acctatagaa caagaattac tctctaggca
     6541 taattctaaa accttctttg ccttattttc aacattctct tttgattgat tactatattc
     6601 aaaaacagca aattcatctc caccaagtct tccaataata gcatcattta cgaatacttt
     6661 agtcaaaata tttgaaaaat gtattagtat ttcatctcct cgaacatgac caaagatatc
     6721 atttacctgt ttaaaattat ctaaatcaaa gatgtaaaag tatccatgtg aaacagtttt
     6781 aagcattttt tttatgtgta cttcaaaaaa atgtctattg tatagtccag tcataaggtc
     6841 tttttctgat tttagtttaa attcatttgc cattgctagt gcttcttgtg ctttttcagt
     6901 tattgtttta gggtaatact catctttcat ttggtcaaag tttggaatag aatggtgaac
     6961 atggataagt ttaatcatat catctgtaac tctgtatacc atagtaaatc gagtatccat
     7021 ttctacatag atatcttttt ctaggtaatc tttttctatt acccaaagag taccatatac
     7081 tataaaagct tcgtctgtga ctttggtaca tgaataccac tcatctataa tctcaaagtc
     7141 tatttgctct gattctgttt ggtctttttc aagacctata ataaattctt ctaatgtttc
     7201 gtaaaattca tgttttcctg ttccaattaa tgcaaggttg tcatctaata aggaaataat
     7261 aaatttaagg ttttcttctt ctggatttaa aatataaata tgccaaattt tttttgataa
     7321 atctcttaat tgtttttgta ttgtattatt ttccatagat taccccctta agatttacag
     7381 tttatatttt aatgtattat aagttgaact cttattacaa tattattata atatagaact
     7441 aaagaaatta catcctgtat aggtaaaagt atatctttta gtataaaaac atactaaaaa
     7501 gttgattatc cgctaaaaca cagtatctat cagcattttc aagaaaacaa acacatcaat
     7561 ttactactaa tttactacca atgaatgagc cttgatacgc tagtattcct agatatgcaa
     7621 agatatggta tggcacatca gtattgaagc aagaaaaaaa ttgaatattt catagactat
     7681 ggaacgccta aaatgacgtt ccttttctat ttccagccgt tcttattgaa cggctatttt
     7741 attacccaaa atgaaaggag catttagaat tatgaaaaag atattgaaac gattgtgtac
     7801 aggcgtactt gcctttgcga ctgtcgttac tgctttaccg actacacttg ttcatgcaga
     7861 aagtaagcaa tactggacgg aaagtgaaga acgtgttggt atcgttgaaa aggtaatgaa
     7921 tgacggttcg attggttcta cctttaacga ggggcatttg accgttgaag gcgaggacgc
     7981 ttactgtatt gacattaaca cagattttaa gaacggctat aagaccagag ctgacgcaag
     8041 ctcccgtatg agtgccgacc agataagtga tgttgcttta tctatcgaat atgtgaagca
     8101 atacaccgcc agccacaatg ggataagcaa taatcacgcc taccttttaa gacagcttgt
     8161 tgtctggcaa agattgagtg ttcacttagg ctggcaatgt gataacgtgc gagcttctta
     8221 tgatgaaatt ccaaaggcaa cgcaagatga agtgtttgct ggtgcaaaag cctttgtcaa
     8281 agaaaacaaa ggacgctatg aatgtggcgg ttatatctat tctggcgagg ggcaggaatt
     8341 agggcaattc tgggcgaagt taaatgttgg aaatgccaaa cttcaaaaaa cttccaataa
     8401 taccagcatt acagacggta acgggaatta ttctatcgct ggtgcgactt acggtgtctt
     8461 ttctgataag gactgcacaa aacagcttgc tacccttacg accgacaaca gcggaaatac
     8521 agaggtttca gaagtcaaag ctactaccgt atatatcaaa gaattgactg caccaatggg
     8581 atttaaagtg gataaaacca tatattcctt aaaagttgaa gctggaaaga cagcgacttt
     8641 gaaagtatct gatacgccaa aagttaccga tactttgatt gagcttttta agattgatat
     8701 ggaaacacag aaagccaacc cacaaggtaa cgcttcttta gagggtgcag agtttacatg
     8761 gaaatattat gcaggcttct acactaaaga caatcttcct gccgaagcta ctcgtacatg
     8821 ggttacaaag acaatcgctg aaacagacag caacgggaca acccactaca tcacaaaatt
     8881 agcagacgca tacaaagtat ctggcgacag tttctatatg caggacggaa aagcggtttt
     8941 acctcttggt acactaactt gcgaagaaac aaaatcacca acgggctact tattagacgg
     9001 tgcatatatg caggcaaacg gtagtgaaga acagataaaa ggcttatacc ttatacagat
     9061 taccgaggac ggcgaccttg ccgtattaac tggaagtaac cagttttctg tatcagacaa
     9121 agtcattcgt ggaggtgtga aaattcagaa acgagattta gaaacgggcg ataccaaacc
     9181 acaaggaagt gcaaccttga aagatactgc ctttgacatc atttccttaa atgataatgc
     9241 ggtattggtt gagggcaagc tatacaagaa aaatgaagtc gtaaaaacaa tccatacgga
     9301 cattgagggt atcgcttcta cttccgctga ccttttgcct tacggaaatt tccgtatcgt
     9361 tgagaaacaa gctccaaacg gttacttgac agacggtgcg aagccgattg attttgcaat
     9421 cacagaaaac ggaaaaatcg tggacttaac agacgaagcc cactctgtct acaatcagat
     9481 taaacgtgga gatattgagg gcgtgaaaat cggtgcaggc acacacaagc gtcttgctga
     9541 tgttcccttt aggattacaa gcaagacgac gggcgaaagt catgttgtga taactgatga
     9601 taacgggcaa ttctccactt cttctgactg ggcttcacat aagcacaata ccaacgcagg
     9661 aaagaccagc gaggacggtg tgtggtttgg aacttctgaa ccagacgaca gcaaaggtgc
     9721 attgccttac gatacttata ttattgaaga attacgctct gaaagcaaca aagattttga
     9781 gcttattcca ccatttgaaa tcgtggtatc aagaaataac cttgtcattg atttaggaac
     9841 tttgactgat gaatacgaaa aagaaatcac tatccatacc acagcaacca gcaaagacgg
     9901 cgaaaagaca atccttgcag gaaaagaggt aacaatcgtt gatactgtca aattagacgg
     9961 acttacaaaa ggcactaagt atcagttaaa aggctggcag atgttaaagg aagaaaacgc
    10021 cgagcttatc attgacggga aacgtgtaga aagcgattat acctttgttg ctgatgatga
    10081 agaaatgaaa gtggaaattt cttatacatt caatgcgtcc gagcttggcg gtaaaaatct
    10141 ggtaacattc gaggaattat acgacttaag caatccagac gaacccgtga aagttgcgga
    10201 gcataaagat attgaggacg acgggcagac ggtacttatt acagaacgta tcatcaaaat
    10261 acatacaact gcaaccgata aggacggtaa caaagagctt gaagtaggaa aagacgtaac
    10321 aatcattgat actgtaacct tagagggctt ggaagttggt acaaagtata aacttgtggg
    10381 ctggcagatg ttgaaagaag aaaatgcaga gcttcttatc aatggaaaac gtgtggaaag
    10441 tgattatacg tttatcgctg acagcgaaac tatgaaagtg gaagttacct ttacctttga
    10501 cgctacttct cttgacggca aacagcttgt aacttttgaa gaattgtatg atttaagcaa
    10561 tccaaacgag ccgaaaaaag ttactgagca taaggacatt gaggacgagg gacagacgat
    10621 tacttttaag gaaaaaccag aagtcccaga gaaacccgaa acgtcaacaa caccagaaaa
    10681 accgagcaga ccaacagaca gtcctaaaac gggcgacaac acaaatgtca tggcatttct
    10741 ggtaatgttg cttacatctg ctggtggact ggctggaaca tatctttaca aacgctgtaa
    10801 aatgaagaaa tcataaggcg gtaacggtat gagaaaagaa aaatcacgct ctccgccgaa
    10861 gctgtcaaac ggcagacgtt tacttattct ggcttgtctg ccaacaacct gcaagcacac
    10921 ggatagtcaa gggtgcgaag cattgattta ccctttacta tctgggggat tgctggtatt
    10981 tccctaattg ccagaataag aaagcacaat caacaaactt aaaaaagaag aaagaaacga
    11041 ggtaaaacac tatggaatta aaatatgtag tgccgaacat ggcacagact tttggaaact
    11101 tggaatttgc tggtgaaaac aagattgaac agcaacgcat taacggacgc ttcgcgcccg
    11161 tatcaagaag ctataacttg tactctgacg tacaaagagc tgatgatatt atcgtaacca
    11221 ttccagccaa agcaggcgaa aaacatttca aaccagaaca gcgagtaaaa cttatcaatc
    11281 caaagattac cgcagatggt aaagcggtca aaggacgagg gttcacaaat tatatcttaa
    11341 cggctgatga tatgttgcca gccgaggaaa gcaagtaagg gggtaaatga ttatgagatt
    11401 agcaaacggt attgtattag acaaagaaaa gacattcggt gtattgaagt tctctgccct
    11461 tagacatgag gtgcgagcag aaaatgatga cggttcaaca agtgaagaaa tcacacgcag
    11521 aacctacgat ttgaaatgta gtgtgcagga acgcatgata caagtatcaa tccctgctga
    11581 tattcccgtc aaggactatc cgtataatgc agaggtagag cttgtcaatc ccgtagctga
    11641 tacggtagca aacgccagct atcgaggtgt caatgtggac tggtatatca aggctgacga
    11701 tattatcttg aagaacaaag gcggtcaagc tgtcaatcca cagaaccatg caccgcaggg
    11761 acagccaaag aaataagctg gcaaactaga ttttcattgt agcggatata agcaactgtt
    11821 ataatatgtg tatgaagaaa cggataaatt gaaatttgtg gaggaaaaag ataatggcaa
    11881 aagaaaaagg ttcattcaaa gaatttctat cggcaatcgc gccagaacac caagcgtttg
    11941 tcgagaaact taataccaaa cttattgagc agggctgtga ccttgtaata aaggaagcaa
    12001 aaagcggtta caccgcttca taccaactag agaagaaaac agtaatgaat tgggtgtttc
    12061 gcaagacagg tgtttttgca cggatttacg gagacaacgc tggaaagtat gaggatataa
    12121 tcgcttcgct tcctgctgat atgcaaaaga aaatgactac ttctcgtgac tgcaagagac
    12181 tgattgaccc taatgcctgt agtgatacct gtgtgaaagg ttttgtttat gccttgaatg
    12241 gcgacacaca taaaaaatgc cgtaatgacg gaatgctctt tgtactgaca aatgaaacag
    12301 cggagcatat agctgggctg gtctgtgcag aggttaatgt gcgtaagtca gctttataac
    12361 gacagaaaag ttaagaacaa taaatcccag tttgacaact tcatatccat acacacaaag
    12421 cagttagtct taggattgac tgcttttttc gtacccagaa aggagtgatt gatttatgaa
    12481 aatgatttta aacaaaggac accgtatcag agccagcgac aagaaccttg tctaccactt
    12541 ttccataggg acgcttctgt ttgtattcat tgcggttatc ctgctactga atttgaaaca
    12601 gctcatgcgt accgattggg agcatttcag cttattagac aacggtttga cgctttccac
    12661 ttacaacttc ataaccctac tgatagcgac tggtgtttat gcattggtcg cttttctgta
    12721 ttaccgcttc tgttatgaca gtttcaagaa gctcctgcac cgtcaaaagc tggcacgaat
    12781 gatactggaa aacaagtggt atgaagcaga caccgtacaa gacagcggtt tttttactga
    12841 cctgcaaagc agatcaaggg aaaaaatcat ctggtttcca aagatttatt atcaaatgga
    12901 aaaaggactg cttcatatcc gctgtgaaat tacattagga aaatatcaag accagctctt
    12961 acggttagag gataaattgg aaagtggctt gtattgtgag ctgaccgaca agacgttaca
    13021 tgacggttat atcgaataca ccatgcttta tgacatgata gcgaaccgca ttaccattga
    13081 tgaagtgaaa gcagaaaacg gctgtcttag gctgatgaaa aatcttgtat gggaatatga
    13141 cgcactccct cacgctctga ttgctggtgg gactggtggc ggtaaaacgt attttctgct
    13201 gacgcttatt gaagctctgt tacatacgaa cgctgttctt tacatattag acccgaagaa
    13261 cgctgacctt gcagatttag gtacggtcat gggaaatgtg tatcacacca aagaagaaat
    13321 gattgattgt gtcaatgcct tttatgaggg catggtacaa agaagcgaag aaatgaagcg
    13381 acacccagac tataaaacgg gcgaaaacta tgcctatctt ggtttaccgc cctgctttct
    13441 tatctttgat gaatatgtgg cgttttttga aatgctgggg acaaaagaaa gtgtgagcct
    13501 acttagccag ctaaagaaaa tcgtcatgtt aggacgacaa gcaggatatt tccttattgt
    13561 tgcctgccag cgtccagacg caaaatattt ctcggacggt atcagagata attttaactt
    13621 ccgtgttggc ttggggcgta tcagcgaatt aggttacggt atgctgttcg gttctgatgt
    13681 gaaaaagcag tttttccaga agcgtatcaa gggacgtggc tattgtgatg tgggaacaag
    13741 cgtcataagt gaattttaca cacccttagt tccaaaagga catgattttt tgcagaccat
    13801 aggctcactt gcatacgcaa ggcaggacgg gacggcgacg tgcgaagcga aaggcgacgg
    13861 cacggactag ccgttgctgg tgtggcgtta gccacgccag cagatacaag cccctcatta
    13921 tctaacagag gggcacaatt acggcggaaa cttccacaaa aacctttgca agcccgcata
    13981 aaataagggc ttttcgtaca tttcgccttt tgtgataaag ccttgaaatt atcacaatat
    14041 cacaaaatta tcacgattgg gggtatgaca actgaatgaa actgattttt taaccgcctt
    14101 gaaagaaaaa cgctgtgcat acggcgtatc gcagacaagg cttgcaatca tggcaaatat
    14161 cagccgtgaa catttgagcc gtatcgaagc aggaaaggta acgctgaccg aggacatgaa
    14221 acatacgctt ttagaagcgg tggaaaaatt caatcccgac aatcccatgt tcctgctgtt
    14281 tgattatgtg cgtatccgtt ttcccacaat ggatataaaa catatcatca aggatatatt
    14341 aaagctcaat atcgactata tgcttcacga agattacgga cattacaaat acaccgagca
    14401 ttaccatatc ggagatgtgt ttgtgtatgt atcgcaggac gaggaaaaag gtgtcctgct
    14461 ggaactgaaa ggcaaaggct gtcgccagtt tgaaagctac cttgtggcac aagagcgaag
    14521 ctggtatgac ttcttcacgg acgcattggt tgagggcggt gtgatgaagc gtcttgattt
    14581 ggcaatcaat gacagaacgg gattgctgga tataccagag ctgatacaaa agtgtgaaca
    14641 tgaggaatgt atttctaaat tccgttcttt caaaagctat ggttctgggg aactggtaaa
    14701 gcataatgaa acggataaat acgatatggg acacacgctg tatatcggtt cattttcaag
    14761 tgaagtgtac ttctgttgtt atgaaaagaa ttacgaacag tacgccaaac ttggtatacc
    14821 gattgaggaa gtccccataa aaaatagatt tgagatacgt ttgaaaaatg aacgtgccta
    14881 ttatgctgtt gtggaactgc tgacacatta tgacgcagaa ctaacagcat tttctattat
    14941 caatcaatat atccgcttcg ctgataaaga accagacaag cgaaaaagcg actggaaaac
    15001 caatgtacgc tgggcttggt tcattggcga ggggcgaccg cccgtaaagc tgacgacaaa
    15061 accagaacct tacacattgg agcgtacact caactggcta caaaggcagg ttgcaccaac
    15121 cttgaagatg ttgaaacaga tagacaagga caatcagaca gagtatctgg aaacgattga
    15181 gaagcaggca aagctgaccg agcgacacca ccagataatc aagcaacaga cagccacggt
    15241 tgaagaaatg gttgccaacg taaaggagtg atgaaaaatg tgggaattat tgaaagactt
    15301 attgctggta tcatttggaa taggtatcgg cgtagtctta atgtgcattt taactgtcgg
    15361 caaagaagct gacagatgta tggaagaaat caaagaaaga gaggacaatt aaatgaattt
    15421 cggacaaaat ttatacacat ggtttttaag taacgcccag agcttagtgc ttatggcgat
    15481 tgtggttatt ggtatctact tagggttcaa acgtgagttt tcaaaactta tcggcttctt
    15541 ggtagttgcc ttgattgctg tcggtttggt attcaatgct ggcggtgtga aagacgtatt
    15601 gttagagctg ttcaataaga ttatcggtgc gtaaaatttt attgtggtgc tgatatgaga
    15661 atgctataat gtggatatga agaaagcgac aaatgggaat tttgggggag aagtaagatg
    15721 tgtaaaatat tgttagtcag accgtctaaa caatatgcag accaagttat gtcatataaa
    15781 gaagaaatgt cacaaaatgg agatagtttt gacggttgtg ctggtcttga aaatgtacaa
    15841 tcatttgagg aatggattga ttttgaagca agattaaaag caaaatataa ggacggatat
    15901 gttccatcag aagtgttttt ggctatacga aaaaaagata actatgttat tggcatgatt
    15961 gattttcgac acccattatc cgacttcctc tttaattttg gtggtaatat cggatatagt
    16021 gttcgtccat cggaacggca gaaaggttat gcgtccgaaa tgttaagatt gattttacct
    16081 atctgtcatg agtttgggga aagcaaagtt ttactaacat gtgataaaaa gaatgaagca
    16141 tcacaaaaaa cgattgtaag aaatggtggc gtgttggaaa aagaaatccc tgatacagta
    16201 ggacttagtg aaagtggaat tatccaacga tactggattt caatatagac tatctttcat
    16261 caaatccagt ttgtaaaagt caataaatac aaagataaat ttgaagttag gagactaata
    16321 ctatgaaagg atttgaacgc aaaaaccaat tattctctct ttgtggattg aattgtgggt
    16381 tgtgtcctat gcttttagga aatcattgtg gcggttgtgg caatggtaat caatcatgta
    16441 aaattgcaag atgtagtctt gaacatggaa tgattgaata ttgctatgag tgcaaacaat
    16501 atccgtgtga aaaatatcaa cacattgatg attatgatat ttttattacg agcagacgac
    16561 aaaaagcaga tttagaaaga gcgaaaatca taggcattga gcaatataat cttgaacagc
    16621 aggaaaaaac acaaattctt tcttacttac tttctaacta taatgacggg cgtagaaaga
    16681 ctttcttttg tgtagcagtc aatttattag aactttcaga attgcaagaa gcaatgaaac
    16741 aaatacaatc aaatgacgaa ttgaccttac taccatttaa ggcacaatgc ttgtatgtgg
    16801 tagagatgtt tcagaagatt gccgacagaa gaaatataaa actgaaactt atcaagaaaa
    16861 agtgattttt aaatttcagt ttgacaactt catatccata cacacaaagc agttagtctt
    16921 aagatcgact gcttttttct tactttaatt tcagattgga gtgatgaaat gaacgatatt
    16981 tttaaggata tgcaggcaaa ggttggctgt gaaaacattt cagacctgcc ctcttacaaa
    17041 cgtaaagtgt ggcatgaaat gaagcaactg aactttgctg actatgaaga aaaacagtta
    17101 gaagattttt ctaactatgt gtttggtatg tcatgccaga ccttaaaaga tgtgatgaaa
    17161 caacagaaag gacgtgagga acaatgcagg aaacaagggt gctggtggaa acgagcggaa
    17221 caactggcga agaaacaaca tcatactggt tcgacctgcc gatagatgtt gccgagtttg
    17281 aagaaaagtt aggtgtcggt gcagaaagtg gggattaccg cattattgaa aaggtactgc
    17341 cctttgctga tgaagtccac gaacatacaa gcgtgtacca gctcaacgaa ttagatttta
    17401 tgtaccgcca gctttcaagc gatatgcagg aagaatatac agcacttctt accgtatatg
    17461 agaacttaga agcactttat atttgcagga acgtgattgc ggtttatccc gactgcaaaa
    17521 gcatgataga tgttgcaaga caaaagctga tgaacgaccc gacgttcaaa catttatcca
    17581 aggactgtca agaatactac tttgactttg aagcctatgc ttctcactta ctggaaaaca
    17641 gacgctatct tgtaacggaa cacgctgtct ttgaactgcc agagtaggaa atgaggtggt
    17701 acttatgatt gatgatatgg cagtttatat cgctaatctt ggcaagtaca atgagggcta
    17761 tttagtcggt gcttggttca ctttccctat tgatgaggaa gatgtgaaag aaaaaattgg
    17821 tttgaatgaa caatgcgaag aatacgcaat ccatgatacc gacaacttcc ctattgagat
    17881 tggggagtac gtttccattg aagaactcaa tgaaatgtat gaaatgatag aggaacttcc
    17941 cgactacatc gtagagtgtt tggacgaatt tatcggtcat tacgggacgc tggaagaagt
    18001 catggaacac aaggacaata tttattatta tcccgactgt gaaaccatga cagacgttgc
    18061 ctactactac atagacgaat tgcaggcact tggagacatt ccacccagct tgcagaacta
    18121 tatcgactat gaagcctatg ggcgagattt agatatgggc ggttgcttca ttgcaacaag
    18181 ccgaggtatg tgcgagatac catattaacg ctggaagatc agcgacaagt attgttgcta
    18241 ctgacagcgt atttctcttt taagggacag tctgaaacat ggctgtcctt ttccattttg
    18301 aaaggagttg aaagaacttg aaaaagatta aaagctacac gggtatctgg aatgtagaaa
    18361 aagtcttata tgcaatcaat gactttaacc taccttttcc agttaccttt acacagatta
    18421 catggtttgt gattacagag tttttcatca ttctgtttgg ggatatgcca ccactttcca
    18481 tgattgaggg agcattttta aaatacttcg gtattcccgt tgctcttact tggtttatgt
    18541 cccagaaaac gtttgacgga aagaagccgt acagtttttt gaaatcacag ataacctatg
    18601 ccctgcgacc caaaatcact tatgcaggaa aagccgtaaa actggataag cagatattaa
    18661 acgaaacgat tacggcagta aggagtgtga actatgtacc cgattaaata tatcgacaac
    18721 aatcttgtct ggaacaagga caatgaagtg tacgcctact atgagctgat accttacaat
    18781 tacagctttc tatctgcgga acagaaattt attgtgcatg acagctttcg ccagctaatt
    18841 gcacagtccc gtgagggtaa aatccatgcc ttgcagattg ctacagaaag ctcaattaga
    18901 agtatgcagg aacagtccaa acgattggtt acggggaaac ttcgtgaggt ggcggtaaga
    18961 aaaattgatg aacagaccga agcgttagta tctatgattg gggacaatca agtggactac
    19021 cgcttttttc ttggcttcaa gctcatggta acggaagaac tgctcaatct gaagaacatc
    19081 aaaaaatcgg cttggctgac attcaaagag tttctccatg aagtgaacca cacgctgatg
    19141 aatgactttg tttccatgcc gaatgatgaa atcaaccgct acatgaaaat ggagaagctg
    19201 ttggaaaata aaatctcaag gcgtttcaaa gtacgccgat tagaaatcga tgattttggc
    19261 tatctcatgg aacatctcta cggcagggac ggtattgcct atgaagatta tgagtatcag
    19321 ctaccaaaga agaaatacaa aaaagaaacg ctgataaaat actatgacct tatccgtccg
    19381 accagatgtg tgattgagga aagccagcga tacttacgac tggaacatga ggacaaggaa
    19441 agctatgtgt cctactttac cgtcaatgcg attgtggggg aacttgattt tccgtcctct
    19501 gaaatctttt atttccagca acagcaattt acctttgccg ttgatacgag catgaatgta
    19561 gaaatcgtgg aaaaccgaaa agcattaacg accgtccgca acaagaaaaa ggaactgaaa
    19621 gaccttgata atcacgctta tcaagctgga agtgaaacca gctcaaatgt agtagacgct
    19681 ttagacagcg tggacgagct ggaaaccgat ttagaccaga caaaagaaag tatgtataag
    19741 ctctcttatg ttatccgtgt gtctgctgat gatttggacg aactgaaacg ccgttgtgat
    19801 gaagtcaaag atttttatga cgatttgaat gtgaagttag tacgtcctgc tggggatatg
    19861 ctggggcttc attctgaatt tctaccagcc agcaagcgat atatcaatga ttatgtgcag
    19921 tatgtaaaat cagacttctt agctgggctt ggttttggtg cgacacagca gttaggggaa
    19981 aacatgggta tctatatggg ctattccgtt gatacaggaa gaaatgtgta cctgcaaccg
    20041 tcccttgcca gtcaaggtgt aaaaggtact gtaacaaatg cccttgcttc tgcttttgtc
    20101 ggttcacttg gcggtgggaa gtcgttctgt aataacctgc ttgtgtacta ttctgttttg
    20161 tttggcggtc aagcggttat cttagaccca aaatcagagc gtggaaactg gaaagaaaca
    20221 cttccagaaa tcgctcatga aatcaatatc gtaaacctaa caagcgacaa ggacaacgca
    20281 gggcttcttg acccgtttgt gattatgaag aatgtgaaag acgctgaaag tctggcaatc
    20341 gacatactaa cctttttaac tggtatttca tctagggatg gcgaaaaatt ccccgtactt
    20401 cgtaaggcgg tgcgttctgt tacccagagt gaaacaaggg gcttgcttca tgtgatagac
    20461 gagctacgcc gtgaagatac cgcaatctca cgcaatatcg cagaccatat cgacagcttt
    20521 acggactatg attttgcaca tttattattt tcggacggaa cggtggaaaa tgctatcagt
    20581 ttagataatc agctcaatat cattcaagta gccgaccttg tactgccaga taaggacacg
    20641 acttttgagg aatacacgac cattgaatta ctatcggttt ctatgctgat tgtgattagt
    20701 acctttgcct tagattttat ccacagcgac cgctctatct tcaaaatcgt agaccttgat
    20761 gaagcgtggg cgttcttaaa tgtggcacaa ggcgaaactt tatctaacaa gctggtgcgt
    20821 gctggacgag ctatgcaggc aggtgtgtat ttcgttacgc aatcttctgg ggacgtgtca
    20881 aaggaaagtc tgaaaaacaa tatcggctta aaattcgctt tccgttctac tgacatcaac
    20941 gagataaaac agactttaga atttttcggt atagacaaag aggacgaaaa caatcagaag
    21001 cgacttcgtg atttggagaa cggacaatgc ttgttgcagg acttatacgg gcgtgtcggc
    21061 gtggtacaga tacacccagt ctttgaagaa ctgctacacg cctttgatac cagaccgccc
    21121 gtacagagaa atgaggtgga gtgatgaaag aaaggataca gagtgcgttc acaaaaaaga
    21181 agatgttccg ctttctcaag ataacggtgt ttgtcgtggc actttccctt atcctgcttt
    21241 cactacttgg aacggtggct catgcaacgg ggcttgtgga cgatacgata aatacagaaa
    21301 atttatattc taaatatccg ttatccaact atcagcttga tttttatgtg gataatagct
    21361 gggcttggtt gccgtggaac tggctggacg gtatcggaaa atcagtacag tatgggcttt
    21421 actgtattac caacttcgtt tggactatca gtttatattt aagcaatgcc acgggctatg
    21481 tggtgcagga agcctataaa cttgatttta ttaacgatat ggcagacagt atcgggcaga
    21541 gcatacagac cttagcaggc gttacttcaa gcggtttttc aacctctggc ttctatgttg
    21601 gtttcctgct tctcattatc ttggtggtgg gaatttatgt agcatatacg ggactgatta
    21661 aacgagaaac cagcaaggca ctttacgccg ttatcaactt tgttgtggtg ttcattttat
    21721 cggcttcttt tattgcctgt gctcccgatt acatcaagaa gataaatgag ttttcatcag
    21781 acatcagtac cgcttcgctg gacttgggaa caaaaatcat gctccccaac tctgacagcg
    21841 agggcaagga cagcgtggac ttgataaggg acagcttatt ctctattcaa gtagaacagc
    21901 cgtggctact tttacagttt ggaaacagca acgcagagga aatcgggaca gaccgtgtag
    21961 aagctcttgt atcggcaaac ccagaggacg aggacggaaa gactagagag gaagtcgtga
    22021 aaacagaaat cgaaaacaac gacaacaaca atctgacgat accgcaggtc gtaaaccgtc
    22081 taggtatggt gttcttccta ttgtttttca acttagggat aactattttc gtattcttgc
    22141 ttacgggtat gatgttgttt agccagatac tttttattat ctttgcaatg tttttaccta
    22201 tcagctttct actttccatg ataccgagct atgaaagcat ggcaaagcag gcaatcgtaa
    22261 gggtgtttaa taccataatg acacgggcag gaattacgct cattgtaacg gtggcgttca
    22321 gtatttccag tatgttttat aacatttcca cagactaccc gttctttatg gtggcgttct
    22381 tgcagatagt atgtttcgct ggtatctaca tgaagctggg cgacttaatg agtatgttct
    22441 ctttgaacgc taacgacagt caaagcatgg gacgaagaat tttccgcaga ccgtatctct
    22501 atatggcaca tagggctagg cgtatggaaa gacggcttgc tggtgcggtt accgctggcg
    22561 gtgtcgatgg tgcggtggct ggaagtaccg tttctaacgc aaggacaaca aggaacaatt
    22621 cagcaaaaga caaccgaggg aatacatctt ccagcatggg acaacgtgca ggctcaaagg
    22681 tgggtgctgt cttagatacg aaaaataaag tcaaggacaa agcaaacgcc atgaaagaaa
    22741 cggtcaaaga tatgccgaca cagaccgctt atgcggtgta ttccgcaaag gaaaaagcaa
    22801 aaacaagtgt atcggacttc aagcgtggca tggtgcagga acagcagtcc agacagtcgg
    22861 gacgcttgga aaagcaggaa cagcataaga agaatatcgc tgataagcgt atggagcttc
    22921 aaaaagcaca agaagcaagg caggcacgaa agacggacgg atcagcgaca acgggagcta
    22981 cccgtcccca tgaacgacca gccacagctc caacttctaa aacaagcaag ccagtcaaaa
    23041 taaattctgc gaaaaaacgt ccgctatctt cggggttttc tgatgtgaaa gctccacaat
    23101 cggcacagcc agtacataga cagaatgtag aaaaagttcc cgtatcagag aaaacacgtc
    23161 agaatttcaa ggcagagcgt acaacaaagg cacatatcgt ccagcaacca aagaaaataa
    23221 cggaaacaac tgaaaagaac cgtaacgcag tatttacgaa aggacagaag aaaaaatgaa
    23281 actgaaacat atcgctctta tcggcagtct gtttcctatc ctcttttcca tagtcctttt
    23341 ctttggagta ctgatgagtg cggacagcga cgacgagaac agcaactttt cttcgggcat
    23401 tacgggtatg aatttatccg cagaagtctt gaaacatcag cctatggtgg aaaagtacgc
    23461 taaagaatac ggtatttctg agtatgtgaa tgtgttactg gctatcattc aagtagaaag
    23521 tggcggtacg gcaaaagatg tcatgcagag ttcagaaagt cttggtttac cgcccaactc
    23581 gttagataca gaaagctcaa tcaagcaggg gtgtaaatat tttgcgtccc tgctttcttc
    23641 ctgcaaaaat caaggaataa atgatttgaa tgtggcgata cagtcctata actatggcgg
    23701 tggctatgtg agatatgtgg caggaaaagg aaagaaacat acttttaacc ttgcagagag
    23761 cttcgcccgt gagaaatcgg gcggtaaaaa agtaacctac accaacccga tagccgttgc
    23821 aaagaacggg ggctggcgtt atggctatgg aaatatgttc tatgtggaat tagtcaatca
    23881 gtatttaact gttccgcagg tatcgggaga actggcacaa aaggtaatga atgaagcatt
    23941 gaaatatcaa gggtggaaat atgtttatgg tggtagcaat cccaacactt cctttgactg
    24001 ttcggggctt gtgcaatggt gctatgggaa agctggtatt tcattaccga gaaccgccga
    24061 cttgcagtat gacgcaacac agcatatccc cttatcgcag gcaaaggctg gggacttggt
    24121 atttttccat tctacctata acgctggttc gaatgttacc catgtgggaa tatatgtagg
    24181 aaataatcag atgtaccatg caggcgaccc gataggatat gcagacttaa atagtagtta
    24241 ctggaaacag cacttaattg gtgcaggacg agtaaaacaa tagaaaggac ttgaaaatat
    24301 gtttaagaag aataagaaac cgacagaaaa tgtcaaagaa aagaaagtgc gtaccgtgaa
    24361 agtgggtaca cataagaaaa ctgtgattgc attgtgggtg gtgcttatcg caagcgtgag
    24421 ttttggcgtg tataagaatt ttaccgctat tgatatgcac accgtacatg aaacagaaac
    24481 catacaatta agacttactg ataccaacgg gattgaaaat ttcgtgaaga actttgcgaa
    24541 gtcctattac acatggaata acagcaaaga agcgattgaa gcaaggacgc aggcaatcag
    24601 cgattatctg acaaaggaat tgcaggactt gaatgttgat accattagaa ccgacatacc
    24661 gaccagctct gccgttactg atgtaattgt atggagtatc gagcagttgg gaacggacac
    24721 tttttctgct acctatgaag tagatcagca gataaaagag ggagaacaga caacagcagt
    24781 caaggcaact tatactgtaa aagtccatgt agacgctgac ggggatatgg taatcataca
    24841 gaaccctacc cttgcaccag caatcgaaaa atcagagtat gagccagaga cactagaaac
    24901 agacgcaagt gtggacgctg atactgtcaa tgacgctacc gcttttctgg aaacattctt
    24961 taatctgtac ccgacagcaa cagaaaaaga gcttgcctac tatgtagcta gaaatgtgct
    25021 tgaacctatt ggcaaggact atctttattc tgaattggta aaccctatct ttataaagga
    25081 cggggacaat gtgaaagtca aggtagctgt gaaattcctt gataatcaga caaaggcaac
    25141 tttagtttcg cagtatgagc ttgtgctaca taaggatagt aactggaaaa ttgtaggata
    25201 acatggaaat gggcttgtat cataattatg gtatgcaagc cctttttata ttgaagtata
    25261 aattaacaat aattataatt aggtacatga atggagggat aaaatgttca aaattggtga
    25321 gttttcaaaa ttgacacaag tttctactcg tatgttgcgt tattatgatg aaacaggttt
    25381 attaaaacct gctaaaattg attcattaac tggatatcgt atgtattcag cagaacaaat
    25441 ctcaacttta aatagaatta tatatttacg tgatagtggt ttcaatgtcg ctgaaattgc
    25501 attagccctc gattgcagta atgataccat tatagaacag cttgataaaa agtctattga
    25561 aattatggat aatatacaaa aagaacaaga aaaactaaag aaaattgaga ttgctaaaaa
    25621 agaattgttg catggcaaaa gtgaattgta ttttaacata accataaaag agataccgag
    25681 ttattgcgtt ttatctttaa gaaaagaaat accaaattat tattatgagg gcgaactctg
    25741 gaaagaactc tctgcttttg ctaaagaaaa taaaataaat atttctgatg aaacattctc
    25801 aatatatcac aatgaagaat ataaagaaaa agatgttgat gtagaattat gtgccattgt
    25861 aaataaaata ggaaaagcta caggagattt taagtttcgt tttaccgaac cagtttctat
    25921 gatggcttgt actatggtat atggagattt ttctaatatt tcttatgctt atcgttcatt
    25981 tgcaaaatgg cttcaagaaa acagtcaata taagatgaaa ggttcaagcc gtcaaatagt
    26041 tcatcgtgga ccgtggaacg agaaaaatcc agagaactat ctaacagaaa tccaaattcc
    26101 tttagaaaat ctcttgtaaa aaatgataat ctgccttgac tttaacatgg tgttaaggtt
    26161 tatcgtttat ttatcaagaa gaaaacggag gaaaatataa tgacaactta taaagtaaat
    26221 tcaataggta ctatctacaa caatgaaaaa ggtgctttca ttcaactaga agcaccatat
    26281 attctagcat taagggcatt agacggattc agtcatataa atgttttatg gtggttcagt
    26341 gattttgata atacggattt tagaagtgta ttggaaacag aacagcctta taaaaatgct
    26401 ccagagaaaa tggggatttt tgcaacaagg tcgccagtac gcccaaatcc gattgcttta
    26461 tcagtagtag aaattatcaa tatcgattat atgaatggaa tgatacaaat tgcttacatt
    26521 gatgctaatg acaaaacgcc tatcttggat attaaaccat acacaccgag tttggatagg
    26581 gtggaaaatc caaatgttcc agattggtgt agtcattggc ctcatagttt agaagaatcc
    26641 gcttcttttg catgggaaaa cgaatttaat ttttaaggag tgatgaacca tgagtttgca
    26701 ggattacatt attgaaaaac cagtaattga tacagaacac cttattttaa gaacaatgga
    26761 taaaaatgac atagatgatt tgaaagaatg gattagtgat atttctttat acgagtattg
    26821 ggggaaacgt ccttcaaaaa gcgaaaaaaa tccagaagta ctctttagta agccagaaaa
    26881 agctactaaa agttttcatt ggggaattgt ccataaacaa gatagcaaag tgattggtga
    26941 aatatggata taccttattg aaaataatag aatggcaaaa gtggctttca gactttctaa
    27001 gcaatatcaa ggtaatggac taatgagtga agcgttgaaa agcataattg aattttgttt
    27061 ttcaaaaaca gaattacaaa aaatatggac agatgtgcat attttaaatc atgcttctta
    27121 caagacattg gagaaagtcg gttttcaaag ggaaggacat attcgagggg gaaaaatggt
    27181 aaatacctat tgcgactact acctatatgg aattttaaaa tctgattata tcactagctg
    27241 atgttatggc taatattgaa gcgacgattg gcgaagcaat gaacaataca gttccaaagg
    27301 tctatgatac gaaaagcatt aaaagatatg tgatgaattt attagatatg gctcattaaa
    27361 aagtaccaaa gaaataaaag ttaccatata gacaaaattg gttttttata tcttttgatt
    27421 tcttataaga taatggcatt gtgcgacaag ctatacaaaa aattttcatg tatactttga
    27481 ttataaatta tacaaggagg tactgatatg tacgcatatg aaaatattga aatgtcatta
    27541 aactatattg aacaacattt aagtgagaaa atcgaaacag agaaactggc ggaaattgcc
    27601 tgtctttcta cattttatta ccaacgcttg tttaaaaagt tagttaagaa atcagttcaa
    27661 gagtatatca aacttcgccg tttagcaatg gtaatagcag aattgaataa ttcagaggaa
    27721 agaatattag acatagcttt aaaatatgga ttttcagacc atgctaactt cacaagagca
    27781 tttaaagaag tttaccatat tactcccgat gaatacagaa agaatgttcc tatgttaaat
    27841 acatttgaca agccagaact ttcttcacgt tatatcatga ttgatgaaaa tgtcccattg
    27901 attgtaggaa atattgtttt ggaaattcaa agaaaaacat taagaacaaa ggaaacatat
    27961 tttggttttg aaaaaacagt aaatattgct gaacaaattc ctgctggaga aagtacagga
    28021 attgatgttc caggtcagct atggcgcgaa ttccatgcaa agaaactgca aaccgaaaat
    28081 gccattttta acaatgatat tgaacttggt gtatctcact tagcaagtcc agataaagga
    28141 acattccttt actttgcagg aggtatggtt tcatcaacag atgtatgtcc taaaaatatg
    28201 gtacaacata cattaccagc aggtgagtat attgtatgca aaattgaggc agaatctttt
    28261 gaagaacttg ttactactgc tttaaatcaa gcaaataagt atctgtttga aacttggctt
    28321 ccaaatcata atttggttac aaagccattc atggcagaaa aatattacaa aggaaatgca
    28381 gagattaatt atatggaaat atgggtaatt cctgtgtaaa cagtaaatgc taaataggaa
    28441 ttatctgacg taataaaaag gaaatagaaa gcttataaag gagggcatag tccgttgtga
    28501 taaacataaa agatataaat tatgtaccta atattagtga aataagcgtt tatattgaaa
    28561 actctgcatt tgatgagttg tatcagcata tgaacaatga atataaagca gtttgcaaaa
    28621 tcgaatacag taaagatgtc tgggcacgag gttggaacgt aaagtttaaa aaatctggga
    28681 aatcattgtg tgttgtctat ccgaagaaga actactttac tgtattggtg gttgtaggta
    28741 ataaagagaa agaagatgtt gaaaacttgt tacctaggct ttctgaatat gtacaggaaa
    28801 tttatcgtaa cactagagag ggaaatggac aaaggtggct tatgatagac ttaaaatcta
    28861 aaaataatgt atatcaagat gtattaaggc ttattgaaat tcgttatgcc ataaaatgtg
    28921 taaaaccgta ataaaaatat ttttcacata cgtaatctca aggtattatc gttgagacaa
    28981 gtaacctata taattatggg aaatgagatt tttagatact taagaaagta ctttaggggg
    29041 cttgcgttat ggaaagattt aagtacaatt ccacacaatc aaatacaatc taataaagct
    29101 ttactaaaaa ttgaatgcaa tacagcttta aaggctctta caaactatat gttttgtgcg
    29161 ggcttttttc atttaatagg aatttggatt cgtcagagag tatattataa aaataaaacg
    29221 ataagggtac cttaaaaata aggtacctca ggctgtagac aaagtcccgg actctcgtct
    29281 gggcttttct tatatttgag ttatccacaa ttcaactttt taagagttat cttattaaga
    29341 aaaaagtgta tgttttccac ccgatagttt tacttctgtg atatttgcag atgccactca
    29401 agtagaaagt ggtggtacgg cagaagatgt catgcagagt tcagaaagtc tgggactacc
    29461 gcccaactct ttagatacgg aaagctcaat caagcagggg tgtaagtatt ttgcgtccct
    29521 gctttcttcc tgcaaaaatc aaggtatcga cgatttgaat gtagcgatac aatcttacaa
    29581 ttatggcggg ggttatgtgg gctatgtggc aggaaaaggg aaaaagcaca cttttaatct
    29641 tgcagagagc ttcgcccgtg agaaatcggg cggtaaaaaa gtaacctaca ccaacccgat
    29701 agccgttgca aagaacgggg gctggcgtta tggctatgga aatatgtttt atgtggaatt
    29761 agtcaatcag tatttaactg ttccgcaggt gtcgggggaa ctggcacaaa aggtaatgaa
    29821 tgaagcgttg aagtatcaag ggtggaaata tgtgtttggg ggaagcaatc ccaacacttc
    29881 ctttgattgt agcggacttg tgcaatggtg ctatggaaaa gctggtatct acttaccgag
    29941 aacggcacaa acacagtatg acgcaaccca gcatatccct ttatcgcagg caaaagctgg
    30001 ggagttggtc tttttccatt caacctataa cgctggttcg tatgtaacgc acgtcggtat
    30061 ctatgtggga aataatcaga tgtaccatgc aggcaatcag cggttatcta acaaagaaat
    30121 tgcaggactt aaatgttgat actattagaa ccgatatact gactagcccc acagttacag
    30181 atgtgattgt gtggaatatc gaacagtcgg gaacgaacac tttttctgct acgtacgaag
    30241 tagatcagca gataaaagag ggagaacaga caacaacagt caaggcaacc tatacggtaa
    30301 aagtccatgt agacgctgac agggatatgg taattattca gaaccctacc cttgcaccag
    30361 caatcgaaaa atcagactat gaaccaaaga caccagaagc agacggtaaa ctggacgctg
    30421 ataccgtaaa tgacgcaacc acatttctgg aaacattctt taatctgtac ccgacagcaa
    30481 cagaaaaaga gcttgcttat tatgtaacgg gaaatgtgct tgaacctatc ggcagggact
    30541 acctttattc tgaactggta aacccagtat ttacaaagga cgggggcaat gtgaaagtca
    30601 aagtagccgt gaaattcctt gataatcaga caaaggcaac tttagtttcg cagtatgagc
    30661 ttgtactata taaggatagt aactggaaga ttattatgta agacattttg ttggcaggct
    30721 ctattttcgg atatagtctg ccttttctgt agcaaatgta ctgcactgac aagctatttc
    30781 attgtatttt aagcaatgga atctttcttg acaattttac cttaaatcat atatttatag
    30841 ctattgactt tctgcttgca ataagctata atatgactat aaaatcaaat cagtcatata
    30901 ggaggtatgg tgtatgtata aagaagttgg catttcttca aataatgttc cgataacact
    30961 ttctatttgg tatacagaaa gaagtagtcc taccattgtt tttttgccag gtactatgtc
    31021 acaccctctc atgtatgaga attttctatg tggattgagc gaaagagggt ttaatataat
    31081 aggtgttcac tatctttcac acggaaaaag ccctaaaatc aaaaaaaatt atataatgga
    31141 agatatgttg cataatgtat atgacgcaac aacttacgga atagaaaatt tcggtgaaaa
    31201 catagctgtc atgggttcca gtcaaggcgg gattttagct gcaatggcgg caggtaggga
    31261 cacaaggtta aaagccgttt ttccacataa tatcatgctg actacattaa aagaaactat
    31321 gagcttaaca aaatatcccg catggtcgca ccgttttatg gggctgatac agtgggggtt
    31381 tcgtgtaggt ggaaaattgc tgcctaacta caaggttcca ggagacgctt atttagacta
    31441 tgacagagta ttttatagtg aacaggcaaa acaagattgc ttaaatgacc ctatgctttt
    31501 accctattat cctttaagat ttgtcggcag cttatttaat gctgacttat cgtgcctgga
    31561 gaatggggct atacaatgtc ctgttatttt aattactgcc aaaggcgacc cgctttttag
    31621 ctttgattat accaacaaag tatttgattt aatccatgcg ccgcaaaagg aattacttgc
    31681 tttagagctt aatcgtcaca tgatttttaa tgaggacacg gacactacaa taaacgcatt
    31741 ggaagctacc ttgcataaat atcttggata gcatattgat tatataatca gtaaatgtta
    31801 tacttttcat gacgatataa atattttagt cataaggagg ttatgagttt gccacgattt
    31861 acggaacaag aaaaagaaat cataaacagt aaacttttga tagagggaga aaagctgttt
    31921 gcattacacg gcttaaaaaa agtaacagtt gatgatttag tagctgctgt aaacatatca
    31981 aaaggttctt tctatgcttt ttatcccagt aaagaacatc tatatgttga aatcaatttt
    32041 cgcttacaaa aagagttatt tacaagtata gaaacaacca ttaaaaagaa aaagtataag
    32101 aaccatagag atttagcaaa ggacgctatc atgctaagtt taacggggtt gattacttca
    32161 cctattcttt cacagattga tttatcttta atggactact tacaaagaaa attatctccg
    32221 aatatttttg aaaaccatat gcacaatgat attcgtatat tggagatatt ggaagattta
    32281 ggggttgtat ttttggttcc gcatacggtt attataaaat ccctgtattc tgttttatcc
    32341 tgtttggaac aattcaaaga ggacgaagaa ctgaatatga tacaaaatct tttagtgaat
    32401 ggtattattc agcaagtagt agcagagtaa caattttgtt tggttatttg acaggcaggt
    32461 agccaaacaa attttttaca gtttatatga ctatatatct ataaacgtca taaagttaaa
    32521 ggaggaatga ataatgattg atgtaaaaaa actgtatttt agctatactg acaaaccgtt
    32581 tgtggagaat gtaagtttcc atgttgggag aggagaaatt tttggttttt taggaccctc
    32641 tggtgctgga aaatcaacta ttcaaaaagt tttaactggg cttaatacaa gatataaagg
    32701 cagcgtaaaa gttgcaggta cagaaataag ggagcataca aatcagtttt atgaaaatat
    32761 tggggtagac tttgaatttt caacctgcta cgagaaattc acagcacgac aaaatctcgc
    32821 ctattttgct tctctatatg aaaagcaacc tcggtctatt gatgaattat tgcatatggt
    32881 aggcttggaa aatgacgggg acaaaaaagt tgctgacttt tccaaaggta tgcgttctcg
    32941 cttgaatttc attaaagcgt tagtccatga ccctgatata ttattccttg atgaacctac
    33001 aagcggtctt gaccctacaa atagtcgttt aatgaaagat attattcttg cggaaaagaa
    33061 acgcggaaaa acgattatca ttactactca caatatgttt gacgcaacag agctatgtga
    33121 ccaagtagcc tttatcgttg ccggaaaagt cagtgcattg gatagcccgc ataatttgat
    33181 tatgtcaaga ggagctgcca aaatacaata tacctactat gataaaggcg agaaaaccgg
    33241 agaatgtctg cttgatagaa ccacagaaga taagttgtta aaaagtttaa tttccgaaaa
    33301 ccgtttgctt tctattcata gtagtgagcc tacattaaat gatatttttg ttgatattac
    33361 agggaggaca cttcaatgag attgggacgt ttaatttgtg gtgatataca tttccaatgg
    33421 aaatatggtt tctattttat ctactttata ttaacagttt tatatgtgtg cgggattgcc
    33481 gctttgccgg aacattggaa aacggatatt gcttctatta tgatctactc tgacccggca
    33541 gcaatgggat tgtttttcat gggagcgatt gttcttttgg aaaaaagtca aaaggtatta
    33601 aatgctatgg tggtatcccc tgtaaaaata tcggaatata tactttcaaa aacagttgcg
    33661 ttgattgcta tttctacggt aatcgcattg atattagggg tagtttccga cagcaatcat
    33721 ttacttggta tcacagttgg tacggctttg acctcagcaa tctttactat gcttggaatt
    33781 attgcagcta caaaaatttc aaacttgaac caatttctca ttgtgattat gccgatagaa
    33841 attgtttgct ttgtaccacc tattgttggt ctgtttgtga agttaccata tctgttccgc
    33901 ttttttccat tcacagcttg catgaacctt ataactggaa aaagcgtttt actatcgttt
    33961 gatatggtac ttgtaattgc tacgttgatt atcctctaca tagttgcgcg acatacagtt
    34021 aagcatatgt ggaaaagttt aggaggtgta aagctatgaa aaggatatta tccagtacca
    34081 tacaggtatt tagacaaatc aagagcgacc ctatgatgtt tgcagcttgt tttacgcctt
    34141 ttgtcatggg agctttgatt aaattaggta ttccctttct tgaaagaata acagattttt
    34201 ctttgcaagc atactatccg atttttgatt tactactttc tatcatggct cctgtattac
    34261 tctgctttgc atttgcaatg attacattag aggaaatcga cgataaagtg tcacggtact
    34321 tttcgattac ccctcttggt aagtcggggt atctctttac aaggttggga gtaccctcga
    34381 ttatttcggc ggtcatcgct tttattgtac tgttactttt ttcattagaa aagttgtcca
    34441 ttggaatgac aatatgtttg gcgcttctcg gctcagtaca ggcaattatt gtttcactta
    34501 tgattatcac attatcaagc aataaattag agggtatggc agttacaaaa ctagctgcac
    34561 ttacattgct cggaatacca gccccctttt ttattgatag ttactaccag ttcgcggttg
    34621 gcttccttcc atcattttgg gtagcgaaag ccatgcagaa tgaggcggtt ctttatttct
    34681 ctataggatt ggtggtagct ttagtctggt actacttcct tacaaaacgt ctgtttcgga
    34741 agctggcagg ataaggaggc atattatgga aaaaggtaga cttctgcaaa acaaaaatat
    34801 aaataaggct attcaggttt ccaaaatcac actaatcatc aacctgagtt tatcgctact
    34861 taaatttgct gccggatata taggaaagtc aagtgccatg ctgtcggacg ctgtacactc
    34921 ggcttctgat gtagtaagca caatcgttgt gatggttgga attaaaatat cagaaaaaca
    34981 gcctgataga gaacatcctt atgggcatga acgtatggaa tgtgttgctt ccattattct
    35041 ctctgttgca ttagccataa caggtgctgg aattggatat tcaggtatca agaaaatctt
    35101 ttcagaacag tacaatacgc tttctgctcc tagtggaatc gcacttacgg cggcagtctt
    35161 atccattgtt ataaaagagt ggatgtactg gtttacaaga aatgctgcaa agcatacaaa
    35221 ttctgacgca cttatggctg acgcatggca ccaccgctct gatgcactct cgtcggttgg
    35281 ttccttaatt ggcattttag gagcgcgttt aggctatgct attctcgacc ctattgcaag
    35341 cgttgtgatt tgcggttgta tcttaaaagc tgctttggac atcttcaaag aaagcattaa
    35401 caaaatggta gaccatagtt gtgacaatgc tactgaaaca aagattaggg aagttgtgct
    35461 tcagcagcaa ggggttgatg gtattgatga attaaaaacc cgcatgtttg gagcgaaaat
    35521 gtatgtagat attgaaatat tagctgatgg taatttagct ctttatgacg ctcatagaat
    35581 tgctgaggga gtccatcaaa cgattgaaaa caactttcca caatgtaaac attgtatggt
    35641 acatgttaat acgaatgaac ttctctattc tgcataggac agggaagatg atttaaggct
    35701 attgaaaggg aaaggaggga agtaggttat ggaacaatac tggtggatta tcattgttgt
    35761 ggttttgatt gtcgttggtt tacttaaaat tgtaattatg aagaagtata accaaaggag
    35821 aaattctaac aatagtccaa aagatagcga ggacgacaag atgtattaag gtttattgaa
    35881 atctgttatg ttgtagaatg atgtttttag atactagaaa gtgctttgag ggggctaacg
    35941 ttatggaaag ctttgagtac aattctttac aatcaacagt cttttgcata gagaaatgta
    36001 tgtaggaaat atggtgcaag gcacaaaaca ccagcaattg agaaatcaac ctatgaacca
    36061 aaggcacact acattttata ctacttgaca agtaaacgaa cgtttactat aatggtttaa
    36121 agaagtaaac gttcgtttac taaagaagga ggttgtaaat gtctgacaca aaagaaaata
    36181 tattaaaaat tgcactacac ttatttgcag tgaatggtta tgaggctgtt tctgttagta
    36241 tgattgcgga agccttggga atgacaaaag gagcattata taaacattat aaaaataagc
    36301 aagatatttt tgactgtatc gtagaacgta tgtatcaaat tgataataag cgagctaagg
    36361 attatcaagt accagaaagc acaatagagg taaccccaaa tgcatacaga aatacaacta
    36421 ttggaagtct aataacattt actcttgcac aatacgattt ttgggtgact gatgaatttg
    36481 cgtctaattt tcgcaaaatg ttaatactag agcaatatag aaatttaaaa atgaatgtgc
    36541 tttatcaaaa atgtcttgta aatggtccta tcagttatat agaagatata tttaatgaaa
    36601 tgattaaaaa aggtgttctt caagagtgtg atacaaaaca gcttgctatt gaatattatg
    36661 cgccattatt tctattgata agtgtagcag acgcagttga aaattgttgg gatttaaaaa
    36721 ctcaattaac tacacacgtt aaacaatttg tcaagaaata tattataaat gagaaagtaa
    36781 aataatcctt tattctgtcc taaatataaa cctaactttt taatttcagc agagaaactc
    36841 aaaatgaata ttgttataag tcacaaatat taactattaa aatattacaa taacggagat
    36901 gagaaaatgg aatatattcg cagtaaaaaa tattttacac ccgacttaca gacaaaaatt
    36961 atggggccga atcctataaa attacaagag gaactattac aaaatcataa aatcccgtct
    37021 ggtagctatg tctgtgacct tggaagtgga caaggtttaa cttctgtatt tcttgcaaaa
    37081 gaatatggat ttgttgtttt tgcagttgac ttatggagtg agccaaaaga aaatgaagaa
    37141 tttttcaaaa aaatgggatt atcttctgca caaattaaat ctgtaaaagc tgacgccaat
    37201 gaacttaaat ttgagaaaga gttttttgat gctgtagtaa gtacagattc atataattat
    37261 tttggacgtg accctatata tttagatgaa aaattgcttc catttgttaa gaaaggtggg
    37321 tttgtttata tcacagttcc tggcatgaag aaagattgcc atgagaaact tccagaagag
    37381 ttattgttat catggacacc agaagaactt gattatatac atgatataac atattggtct
    37441 aacataatta gtcattgtaa aaaggcagat ttaatttcaa tatatgaaat ggaaagtaat
    37501 tcggaagttt ggtcggattg gataaagcaa aataatgaat atgcaataaa tgaccgtaag
    37561 gcaatagaag ctggcagtgg agattatctg aattttattg ctataattct acaaaaaaaa
    37621 taaattcttt agaaccaaac actataattt gcgttctaat atgtttaccg attgacatca
    37681 ataattttat ctttgagggt aatgatttga aaatagaaga ataattccag tttgtaaaag
    37741 tcaataaatc caaagataaa tttgtatttg aaaggagttt tgaagaatga aaaaatatga
    37801 gtatgtagaa attaactata atgcgaaagg agttgtattc ctttgtactg ataaacacaa
    37861 agaaattatt gacagttacg caaaaacggg gtatcactac gcagggtttg tacccacaga
    37921 aattgacgca aaaggctgta tgcgaaagat agatttaatt tttgaaaaag aagattaact
    37981 tattttaggg agttggttct aatgatagga cttaaaagac gagatatgaa ttatattatg
    38041 aaagttggtg ctaaagctca aggtgtttct gtatctgaat ttagggctat gctacaagat
    38101 accatagaca aaactatgga aagtacagac ccagacgtgc aggcaaattt taaacgatac
    38161 tttggaaata agcgcccaac accagaggaa tatatttata cgattaccaa aaagacaaag
    38221 gttaaggttt aaatgttgta aagggggctt gccgtatgaa acgctttatg tacaattcag
    38281 tatattcaag cccaatttaa cagcatacta tttacaatta aatacgctat accagtagct
    38341 cgtacaaaat ttatattttg tgcgggcttt tttaataccc gaaaagtttt tttaattatt
    38401 ttcatttttt tggaagcaaa tcacaccttg ctgtacagat atggtgcgga aagagggata
    38461 aacacttttc aattcatagc tggaaaggag gtgagattat gaaaccgtct gacttccaaa
    38521 aaacaattca gtgtcagttt gaatgtaagc tgaaaaaagt tgtcaaaggt attatctgga
    38581 actaccgtaa agaattaaaa cgaagaaaaa acaaggaaat atcttttagc gaattactag
    38641 acattacggt agaaagtctg gcaacatggg acgactacga aacaaactac acaattttta
    38701 atgtgtgtgg catagatatt aaagtccttg atgatgaact tgcagaagct ctcaaacagc
    38761 tacccgaaaa aaaacgtaac aatgtattga tgtattactt cttggaaatg agtgatacag
    38821 agattgcaga tattatgaat tataccagag gggctgtgtt caaacacaga caccgtgcac
    38881 tagaaactat gaaagagata ctaaaggaga agaaatagaa atgaagaaaa agtacaagca
    38941 cccgtcttat tatttgattt cattagctat ggatggggat gaaactgcga ttgagaaatt
    39001 actggcattc tatgacccgt acatatcaaa gtgttgttta cgtcctttat atgatgaata
    39061 cggcaatgtt tatattgttg ttgatatgga attaaaggga cgtattcgag aagccttaat
    39121 caaaatgatt ttagactttg agattgatat agatatagaa accgaagaag aataagcaga
    39181 taccagcagg gcgtgattgg ttaaattccc ttctttcctg ccctgctcct gcttttatac
    39241 atggaagcct acggctttcg ccgattgttc ttagacaact gaataaagca gacagatacg
    39301 tttgtgatac agcgagccac ggggactgta cgccatgacc tttccgacaa gaaagcgagc
    39361 gacccacgca gttgatccga gagcgacttg tggaatggtc gttttgccat gacctgctat
    39421 cacagaataa tgatacgtcc gcatggtgcg gttctgccca caagaatggg aatagttgag
    39481 atactaacgg agctttccaa agccgtctgt ctgcttatga taagacacac agaaaggagt
    39541 tgttgcaaca tgaacaatac tgatgtgccg atttgggaaa aatacaccct tagtatagaa
    39601 gaagcgtccc gatattttcg tatcggggaa aagaagttgc gtaaacttgc cgaggaaaat
    39661 ctggactctg gttgggttat cgttaatgga aaccgtattc agataaagcg aaaacagttt
    39721 gaaaaaatta ttgatacact ggacgaaatc tgatgtctta gagccgtaga tatggtataa
    39781 tattagtagc gtatcacggc tcattccatg acggaaagga gctttacact atgtcaaaaa
    39841 tgaaaagaca agacagtaaa ggacgcaatt tgcgtcttgg agagagccag agaaaagacg
    39901 gaagatacgt ttataaatat accgatatat tcgggaagcc acagtttgta tatgcttgga
    39961 aacttgtacc cacagacaaa acacctgctg gaaaacgtga tgatatatcg ttgagggaaa
    40021 aagaaaagca gataaaaaaa gaccttgacg acggtatcga cacagtgggt ggtaaaatga
    40081 cagtctgcca gctttacgaa aagaagaaca atcaaagaaa gaacatcaag agaaacacaa
    40141 aaataggacg acagtatctt atgaacgcac tggaaaatga cccattgggt atgagggcga
    40201 ttgatacggt caagcagtcc gacgctaaag aatgggctat cagaatgaat gacaaaggtt
    40261 attcctacaa aacgatagat aactataagc gttccttaaa ggcttcattc tatatggcga
    40321 tacaagacga ctgtatcaga aaaaatcctt tcaatttcaa gttgagtgat gttctggaag
    40381 atgatacgga agcaaaggtt atcctaacac cagagcagga agaaaaactc cttgccttta
    40441 tggaaacaga taatgtctac agcaagtatc gtgatgaagt tattcttctg ctggaaacgg
    40501 gacttcgtat ctctgaatta tgcggactga caacacacat tgatatgctt aaccgtgtga
    40561 ttaacataga ccaccagcta ttgcgtgata cagaggtggg ttactatatt tccacaccta
    40621 agacaaaaaa cgggaagcgt gagcttccat tgacagaaag agcatatcag gcacttgaac
    40681 gaattttaaa gaaccgagga aaagcacaac cgcttgttgt tgacggttac agcaacttct
    40741 tgttcttgaa ccgtgaggga ttgccaaagg tggcaggaaa ttatgaaagt atggtgcgtg
    40801 ggctgattaa gaagtacaac aagacgcata aggacaagtt gccaaacatc acgccccatt
    40861 cattcagaca tacttactgt acaaacatgg caaataaggg aatgaaccct aacaccctgc
    40921 aatacatcat ggggcacgca aacataacca tgacattagg gtattatgct cacggtactt
    40981 tccagtctgc aaaggctgaa ttagaaaagc tggcttctta atatcgaaaa tgtatcttac
    41041 tacttattta ctacttttgg ttgcattttc atgctggtaa atgccatctt atgcgaggta
    41101 tcttccaaaa ggaaaatgcc aataaagccc gaaaatatgg gatatatcgg catttaccaa
    41161 cttatgaaaa gataatcaga aatttagtaa gtttttacaa taaaaatatt acagtaaaat
    41221 gaccagtttt aatatacgta ttatattttt aatttataaa cgaatattat aaatgaaatt
    41281 taaattcaac cttgaagaaa taagctaaat gtattagtat attaatccat gcttgaacat
    41341 aaataacctt tatgatatga tatcaataat aatattatga gaggagtgcc ttgatgtcgg
    41401 agttatataa tggtaaccaa caattaaata gttggagaca catatattct gtataatcag
    41461 aaatttcaag tgtattttta atacatttaa tttttgtgtc ttattctgcc tttatagata
    41521 actgacatat gacaacaaat agtatttaaa atatatatta ctttgttaaa ttatggtgta
    41581 gtaggcttta atctgctaca cttttattaa ttttgtatag tattattttg ataactattt
    41641 tgaaatagag agcattaatt tttctagtcc acaggagttt tctgtttttg tgctgttaaa
    41701 tataagcaca gaaaaaggca gatatatacc tgtggacttt ttttatataa attatgaaat
    41761 atggaggtag ttaaaatgag tataattgaa cttaatgaac tgactaaatc ttatagaaga
    41821 tttgagaaaa aggcaggatt tatggggagt gtaaagtctt tatttaatag gaaatataca
    41881 gaaaaaacag ctatatcgtc atttgattta aagatagaag aaggagaatt tgttggatta
    41941 ataggtcaaa atggagctgg caaaactact cttattaaga tgttaactgg tattattgca
    42001 ccgtcttctg gagaaatatc tgtaatgggt tattacccta acaaattaga aaataatttt
    42061 aaaaaacaat atgctgttgt tatgggccaa aaaagtcaac tattctttga attgacacca
    42121 gcagatacgt ttcttttatt taaagaatta tatgatatat ctaatgaaga atataaaaaa
    42181 aacttggatt actttatcga attatttgag gtatctgact atttaaatgt tcaagttagg
    42241 acgctttctt taggagaaag aatgaaaatg gaactaatag tagcccttct acataatcca
    42301 aaaatattat ttttggatga accaactata ggcttagatg ctatagccca aaaacaaatt
    42361 aggtatttcc taaaagaagt aaatgaaaca aaaggaacta ctattatact tacttctcat
    42421 tacatggaag atattaagag tctctgtagt agaagtattg ttgtagaaaa tagccgtaaa
    42481 atatatgatg gttctacaga gaaactattt aatcattttc aaaaatataa aaaaataaca
    42541 gttagttttt ccaatgaaac taacattgta tttccaaaag attgtaaagt tgttgaacaa
    42601 acaaatcata agacatcagt aattgtgcca aaagaaagag gaaatgaaac gattaaatgg
    42661 atttttgata aatatgatgt taaagatgta atgattgaag aagaagatat aggtagtatt
    42721 gtagagagaa tatataagaa aggaagtgat gagtattatg agcaactata atgaagcaag
    42781 ttcaaatgat tcaatgtttt ataaatttaa acagagtatt agaaggtatt atgaagtagc
    42841 caaaattaat tttaaagtgc aacttatctg gagatttgat gtggtggtta atatggtgtt
    42901 tacgattgta aaaattttat ttgcttatat tttatggaaa gcaatttttg gtgaaaataa
    42961 aatagtctct ggttttacat ttaactctat gttgtcttac tatataataa gctcattttt
    43021 agcaggtgtt gatatgtcaa agagtaccag tgaagagata tgtaatcgtg taaaattggg
    43081 caccttttca aagtacatga ttattccaat taatgttcaa aattatttta ttgctaaaaa
    43141 ttttggaatg acatgttttt attttgtatt taatatgatt tctgcatttg tatggatttt
    43201 tatatttaaa atagattttg cctttacatc aaatccagtt caaatattaa tttcaatttt
    43261 gatgattttt ctagggttta tttttatgat gaaactccac ttctttattg ggattttgtc
    43321 atttaaattt cttgagatta gtttttttag aatgataatt gaaaatattg ttgaatttat
    43381 tacaggaaca atgataccac ttgtactctt accagaaact atagtaacag ttatgaaggt
    43441 atttccattt tattatacaa catatttgcc atctatgtta ctaataggta gaaacagcaa
    43501 tgaggcaata caaggattaa ttattctagc attatgggtt ttattttttt ctgtagtaaa
    43561 taaagttatg tataaaagaa tgagaacatt atttgatggg gtgggtatat gatgaaaaaa
    43621 aatttgaaaa taatatttat gttattaaaa atgaaattat caaagatgat ggtatttaga
    43681 tttgattttt ttggagcatt ttttgtagat agttcattat ttatattaca acttctaatg
    43741 tttaatagta tatattctca tgtagactca atagggggat ggcaacaagg agagatgtta
    43801 atatttatag gaactttctc attaataaat gcgataaata tgacgatttt cttttttggt
    43861 atttatgata ttccacgaaa aatccaagaa ggagaattag actactatat tacaaaacca
    43921 ttagacccac tatttaggtt aacatttgaa aatattaata ttggttcatc accattaatt
    43981 ctagcaagta tacttattgt tttatatgga atatctgagt taaactcaga gataacttta
    44041 gttatgataa taatatatat tttcatggtg ttattgatga cattactttt ttatgatgta
    44101 tgtatcatac ttagaacagt ttcatttttt gttatatcat cttctgcgat aatgagatta
    44161 gaagataatt tattgccaat gaatatgaaa attcctggtg taatatatga aggtgttttt
    44221 aaagtattat tctatttaat cttaccatat ggaattatgt cgacaattcc gactcaaatt
    44281 ttggctggga caataacaat taatggatta atatactcaa ttttaattgt atttttgttt
    44341 acattgttta tgtggagatt ttggaagtta ggtatgagac attataaaag tgcaagtagt
    44401 taataaatta gtaaaaatag acaatgtata actatatata aatacgtttc atatgacact
    44461 aaactatgtg aacaagtatt tgcgctaaag gcactgaaag ctataaaatt attaaagtaa
    44521 ggatttaaaa gtttctttag tagaaatata attgtgtaaa aaagcaagat taattaaaac
    44581 tatatccaaa taattagata taattcttat gcagaaagga ggtctgtaaa tgtctaattg
    44641 tttggaatat attttaaaaa taattaatgt acagaaaaat aaacaaaatg agtgatgtat
    44701 attattcaga aaatcatgaa caagataatc tttatgagca agataattta ctggcacaaa
    44761 gatttaattt atttactcta ttgaagtttg catttcctac tatttttatg atgattttta
    44821 tggggttgta tacaatcgta gatactatat ttgtatcaag atttattaat acaaatgctc
    44881 tatcagcaat taatattgta tgtccaatta ttaatcttat agttggaatt ggaactatgc
    44941 ttgccactgg aggaagtgct attgtagcac gtaagatggg aaatggtgat attgagggag
    45001 caaataaagc tttttcttta atcgtattat caggagttgt aattggggta ataattactg
    45061 gtataggtat tgtatttatg gattatatca ttatatggtt aggagcaagt gaagttttaa
    45121 ttaaatattg tcgttcatat ctttttatta tcttggtttt tgcttctgca aatatactac
    45181 aagtattatt tcaatgttta tttgtaacag ctggaaagcc tcaatttgga ttatggctag
    45241 tgttaggtgc tgggtgtgct aatataattc ttgactatgt gttcattgtt ctgttacaaa
    45301 tgggtattag tggagcagca cttgcaactg gagtaggata cctaattcct accgtttcag
    45361 gtatggtttt cttttcaaaa aataaagggt tacttaaatt ttgtatacct agactagata
    45421 aaagaatatt atttgaagct tgttttaatg gttcatctga aatggtgggt caactatcaa
    45481 cagcagttac tactttttta tttaatgcta tgatgatgaa attaattggt gaagatgggg
    45541 ttgcagctat tacaataatt atttattctc aatttctttt aacttcgctt tatataggtt
    45601 tctcaatggg tgttgcacca ataataagtt ataactatgg aagtgaaaat aatacgcagt
    45661 taaatcatgt ttttaagatt tgtatattat ttatttcagt agcatcttta ttggtattta
    45721 tcttttctat atcatgttca ccaatcattg tagagatttt ttctcaaaga ggaagccgag
    45781 tttatgaaat tgcaaggtct ggattttata tattttcttt tagttttatt ttttgtgggt
    45841 ataatatttt tacttcgtct atgtttacag cattatcaaa tggaaaggtg tctgcaatat
    45901 tatcttttct acgtacattt ggatttataa ctataggttt attgatattg ccaaatatat
    45961 taaatataaa tgggatatgg ttagcagtac caagtgctga gattttaact ttatttttaa
    46021 ccattttttg tatatgtaag tataagaata gatataaata tatgtagata ttaaataaat
    46081 agtttaggta gatattttat gttttaataa gtaattcaat tagtgggaga tatatactat
    46141 tgaaaattaa agttataatt gatataaaga attaataaaa ataacatata aaagtttaaa
    46201 agggagttga acttaaagtt tgattagaaa aagtcaattt taagacaact ccttttattt
    46261 tactattgta ttaatctgta tctgtaagtg tattagtcta atattataaa ctttggatga
    46321 aattaatttt ctataggttt taatgtatca tacactttat tttaaagaaa cttatcttaa
    46381 ttttcatagt gattgaattt gtctattaga gatgttacta ttagtaatgt aaaagcaaga
    46441 gtagtgaata taaaaaacaa gatagtgata ctgcaagatt agaatataat aaatttttat
    46501 ggagagattt caatatggat acatgtttta ataaaaaaat attacttgtt gatgatgaaa
    46561 aggatatagt agatttaatt gaagaagtac tcataaatga tggttttaaa aacattatta
    46621 aagcttataa tggtctagat gctatttcgc tgtgtaagat agcatgtcca gatgttgtca
    46681 ttcttgatat aatgttacca gatatagatg gtatagaagt gtgtaaaaaa attcgagagt
    46741 tttcatactg ttctattctt tttttatcat caaaaaatga tgatattgac aaaatactag
    46801 gtcttagtag tgggggagat gactatataa caaagccatt cagtccaaga gaaattgctt
    46861 ttcgagttaa agcgcaatta cgtagacaac agtatcaaag cattgtacca tcagatagtg
    46921 aagttattaa aataggagat attactattg atatagaagg aaaccgtgta tataaagata
    46981 gaaatgagat tgagttaact ggaagggagt atcatctttt aagctacatg gctcaaaatg
    47041 taaataaaat tataggaaaa gaaagactct atgaacaagt atggggagta tatagtagta
    47101 tttgtgacaa tacaattatg gtgcatatac gacatataag agaaaaaatt gaagataacc
    47161 cctcaaatcc taaaatactt ataacagtta aaggactagg atataaactt gtgaatagaa
    47221 tcgattagaa tagattttaa tattaatgaa gatatctcaa gttgaatttt tagttaatga
    47281 ggcattcttt tatgggtaaa atcaaatata ttttcgttat ctcaacaatg aagaagaggt
    47341 ctatctctat tttgagatag cctcattaag tttatatcct ggtaatgtac tttatcttct
    47401 aataaaattt ttttagttta tttgcagtga aacattaact agaacattaa aatttgcatt
    47461 gaaatactaa ttaaataata ttgtatattg tgtataaact tatactaatt gtttttctat
    47521 attaaatact ttatcaatcc aacctttata aaatttttct tcatgggaaa ctaggattat
    47581 acttcctcta aactgtttca aagcattttg taaagcatcc tttgtctctg catcaaagtg
    47641 attggttggt tcatccagga taagaaaatt acaaggagat aacatcatac aacataattt
    47701 aaccttagat tgttcaccac cacttaaagt agatacactc cttgaaacat gctcttcctt
    47761 cacaccacat ctggctagat gacgtctaat ctcttttgta tttaattttg gatatttatc
    47821 tgctactatt tgaagtgggg ttttatcagg attttcccat tttaaatctt gctcataata
    47881 accaatttta acttgttctg aaaactggaa atctccagat atacgaggaa tatctttgac
    47941 taatgtcttt aacaaggtag atttaccgat tccattaaaa ccagttataa ctattttttg
    48001 acctccatca acagagaaat ttaattttgg aagtaaagaa tagtagtatc caacttcaag
    48061 atttgttata gtaagagcat tttgagccga tatttcaatt tctgaaaatt gaatatttgg
    48121 ttttcctgta aaactaggag gagctattct ttctatttta tccaattgtt tttgtcttcc
    48181 tcttgcaatt tttgaattta ctccagcttt atttttacgt ataaatgttt cttctttttc
    48241 tattttcttt tgttgtgctt gataccttct aatataatct tctcttaaat gtgatttttg
    48301 tttaaggaac tctgaatatt ttccatgata tttcttaatc atgccaaatt ctatatcaag
    48361 aatacctgta gaaatttttt ctaaaaagtc aaaatcgtgg gaaactacaa taaatgcccc
    48421 attaaatgta tttaaataat ttgagagcca ttcaacatgt tctttatcta aaaagtttgt
    48481 tggttcatct aataatagaa catcatggtt agaaagtagt aattttgcta aaattacctt
    48541 tgccctttga ccaccactaa gctctccaac aacacgattc attcctattg aatctaatcc
    48601 aagaccattt gctattttat ttatttcatt atctatagaa tagaaattac ttgctattag
    48661 ttgttcctga taatctgatg ctttgattag atattgctca ttttcagata tagcactttt
    48721 ttgatagagt aaattcattt ccttttctat tttataaagt tcttcaaatg cagtgtgtaa
    48781 atagagtgat attgttgtat ctctattgat ttctgcatat tggtctaaat ggcctatatt
    48841 gatattaggt tgccacttga tttctcctga atctggaaca acttctccca ataaaatttt
    48901 aattaatgta cttttacctg taccattttg gccaactaca cctatatgtt cacctttgaa
    48961 taaatcaaag ttagctttct catacagtga tttatccata aaacattgtg ataaattagt
    49021 tacttttaat aaactcataa ttcaaactcc ttttctttta ttaaaaaatt tatggaaata
    49081 aaaaaagcag aggaatggca ttgtaaaatg ccgacctctg cttttggaaa aacaaaaggt
    49141 catagacaag cattgttgtc tatgaccaga aaatctatat tatctatcgg caaataggta
    49201 attttaaata agcatagcca aaaaactata ctaaacctat atttgccgat gaagatagaa
    49261 agctttaagc tttcagatta acctctacac aacgcttcat cgtatacatt tgtgtagagt
    49321 taagttttct aacaagttta actaattcta tataaatcat aattattcac atcctaatct
    49381 tagtattaat tctagtttat ctgtatttaa attgtacttt tgtactttgt ggattatctt
    49441 atttatttaa aatatgtttt attaattata gcttaagtta tgttatcata atacttagtt
    49501 atagtcaata agaaagttat atttactatt attatattaa tttagtaaat ctatcattac
    49561 aaagttattt gaagactcgc tatcaataaa attttatagt ctataagaaa attaagatta
    49621 tgagttttat aattttgata tagggatact attttcataa atattacaac atgtagaagt
    49681 atatgtctat tattgttgaa ttttcaatga tatattaagg ttctaatata ttatacaaaa
    49741 taaaaacatt aattcaactt aaataatttt aagtattata tgtgttttga ttaaagaaaa
    49801 ctaaaatttt aaggaaaaat aacatatttg tattaaattt tattgaaatg attaaattta
    49861 tttacttaat gtaaattatt agctaaaatt gtattaaata attaaggagg tgagtagctc
    49921 tggatgaaat agatttaaaa ataattaatt ctctaaaaaa gaatagtagg gcttcaacct
    49981 ctgaaataag cagacaaatt aatttatcag taccagcagt ctcagagcgt ataaggaaaa
    50041 ttgagcagtc aaatttaatt caaaagtaca ctattcaaat taatcgggaa aaaacaaaat
    50101 ataagctttt ggcttttata tttatcaata ctgatttatc taaaagtatg aaagaattta
    50161 agaaaactat attggaatat gatactgtat tagaatgtca tcatatttca ggagagtatg
    50221 attacttaat aaaagtatta gcagaagata caagtaatct tgaagagttt atatcagtga
    50281 ctttgaagga aattgggggg attcaaaaag ctaatacaac cattgtttta tctacaatta
    50341 aagaagaatt taatacaata aaagatattt tttaaataat ttactagtaa agtgttaatt
    50401 atattttagg aggaaaatag aatatgaaag ttttagtttt tttagcaaaa ggttttgaaa
    50461 ctatggaatt tagtgtattt gtagatgtta tgggatgggc tcgtaacgat tatgggcatg
    50521 atattgatgt agtgacttgt ggatttaaaa aacaagttat gagcacattt aatatacaag
    50581 tattagttga taagacgata gaagaagtat gtgttgatga ttatgatgct ctagctattc
    50641 ctggaggatt tgaagaattt ggattttatg atgaagcata tgattcatct tttttaaatc
    50701 taataaggga gtttaattca aaagaaaaaa taatcgcttc tatttgtgtg gcagcattgc
    50761 cagttggaaa aagtggtgtt ttaaaaaatc gtaaagctac tacatatcat ttgaaaaatg
    50821 gtaaaagaca aagacagtta agtgagtttg atgtaaatgt agttaatgaa ccaattgttg
    50881 tagataaaaa tattataacc tcatattgtc cagaaacagc tccacatgta gcttttaagc
    50941 ttttagaaat gttgacatct aaagaacaaa tggatgaagt taaattagca atgggattta
    51001 aactgtaatg gtttatagat tttctaagta atcagaaccc cagtcacaca tggaatctaa
    51061 aattggaata atactctttc caaaatcaga tagcgagtat tctacttttg gaggaacaac
    51121 aggataaacc actcttataa ttaaaccatc tgattctaat tcacgtaatt gttgagttaa
    51181 catcttaggc gttgctttag gaattaattt ttttaattca ttgaacctca atatagtgtc
    51241 tttaagatgc caaagtataa gtgtcttata tttaccacca atcaatgcaa gtgttgcttc
    51301 tattgggcaa ctgtaattat ctaaacattt tttcattttt gggctccttt atttatttta
    51361 aagtatcttt ttaggtacta tattacaaaa aagtgcatta ttgtaatata gtaccatagt
    51421 gataaaatta tagcataggt taaataaatc atcaacttag cctaataaaa caataggagg
    51481 taaataaatg aatttcgttg aacttgcaaa aaaaagatat tcttgccgta attatcaaga
    51541 tagaaaggtt gaaaaagaaa aacttgagaa ggttttagat gttgcaagaa tagctccaac
    51601 tggtggtaat cgtcaacctc aaagattgat tgttattcag gaaaaagaag gtataaataa
    51661 actttctaag gctgccaata tatatgatgc tccattagca attcttgtat gtggagataa
    51721 agacaaagtt tggacaagac cttttgatgg gaaacaactt acagatattg acacaagtat
    51781 agttacagac catatgatgt tacaggcaac tgaacttgga ttggcaagtg tttgggtgtg
    51841 ctattttaat ccagatataa ttagagaaga gtttagtttg ccagataatc tagaaccaat
    51901 aaatatatta ttgatgggat atgaatctaa gataccagag agcccagaga gacatgaaaa
    51961 aactcgtgtt cctttaagtg aaattgtttc ttatgaaact ctttaattat aaaattaagt
    52021 tattcatagg ataaatgtaa taaagtgttt attataaaca aaattcaaaa taaataatat
    52081 ctacatataa ttaattttag tgaagtatat ctagtttaac tagaatgtat acttcactaa
    52141 tttatttatt caaaatattt atgaggattt taatatatta actaaaatat atcaattgaa
    52201 ttaactttta aatctggaaa agcacttgaa atatagtgtt ttatgtcatt taaatggtct
    52261 tgaatttgct tttctgatat tttatcaggt ccatatacaa aatacataat atttttagta
    52321 tcttttgtta tagctgttcg attatcaggg ccattaagaa tactagataa tataccaata
    52381 ttatctgata aaaataaatc atcttttgtt aaaatatgtt ctttaccacc tataccataa
    52441 aaatgttcac ttccatttgc taattctatt ttcaaaggta attccatttt atcaaggtca
    52501 tgacctgctg ttagaagaag atttttcaat tctgctaaaa acattgactc aattgttaca
    52561 cctacatctg ggataccttt tgattttaat agaatagatt ctaattgaag caatacaggg
    52621 taagttttct tgaatttttt ataatacttt atataacttg aaacaggctc tgttttaatt
    52681 ttttctttac gattgtattc tgggtgttgt tctataatat ttttaattgt agagttttta
    52741 agaactaaaa aattttcttt attaggtttt gaatgtgtaa tatttataac cataactcca
    52801 aatttaatat tgggataaat ttctttaact ttatgtgata tttctaacat aatatagact
    52861 cctttttatt ttatatttta tgatataatc ttagcaaatt aaaagtggat tttgtagttc
    52921 catttttata gtaattttat agaaccactt ttggaggtat tagaatgtgg attacgatta
    52981 atagaaatag taatatatca ttatcaagac aaatttattg tgagataaaa gatatgattc
    53041 tggatggaag cttaaagttt gagcaaaaac tgccatcatc taggacttta tcaaaagaac
    53101 tatctgtatc tcgtaataca gttttagatg catataatca attaattgca gaaggatatt
    53161 tggaaacaag gaatggatat ggaacaattg tagctaaagg aatatcaggg cataaagttg
    53221 attttaagtt aaatgatact tttagagatg aaaaagattt acatactaat aaacaaaaaa
    53281 attatataga ttttcgttca ggagttccag aattaaaact atttccacgc aaagaacttt
    53341 caaagctata taaacaaata ctgtatgatt tatcagattc ggatttcaga tatggaagta
    53401 cagctggtgt atgggtttta agagtggaaa tatctaagta tttatttcgt actagaggaa
    53461 ttagatgtaa tcccaaaaac attatgattg tttctggttc aacacaaggt ttatctttaa
    53521 tttcacagct tttatacaaa agtaataaaa aggttgctgt agaagaccct attcataaag
    53581 gcttattaaa ggttatttca tctattggat attcagttgt tggaattaaa gtagatgata
    53641 agggaatgaa taccaactta ataaaatcaa taaaggggat ttcttttgtt tatactacac
    53701 cttctcatca atacccatta ggagggattt tacctattca aagaagacta gagcttatta
    53761 aatgtgcaac agagaatgat tactatataa ttgaagatga ttatgatagt gaatttagat
    53821 atgagggtca accaattagt tctctttatg aacttagccc aaataaagta atttacatag
    53881 gttcttttag taaaatatta tctccagcta ttcgtgttgg atttatgtta ttaccagaca
    53941 atattttaga aagctataaa accttaaaaa tgtactctga tgttcataca gaaacaatat
    54001 tgcaacatgt attagctgaa tttattggaa atggaggttt agagaagtac atatttagaa
    54061 tgaagaagtt gtacaataga aaacgtaagt acctcataaa agttttaaac acggaattta
    54121 aaaatgaatt taaaataaaa ggtgaagcag cagggcttca tatagttgct catttctata
    54181 atgttaaatt tacggaaagt ttaattcata aaatttttga agcaggtata aaagtttatc
    54241 ctatacaaca atatgcaatt gagaatatat tagaatatga ctatgaaatt atactaggtt
    54301 atgctcatct tagttttgat gaaattgaaa aaggtgtaaa aatactcagc aaaataatac
    54361 atatgaatca aggtacatag ataaaacatg tagcaatcta catataatag atgtaattaa
    54421 gtttaaactt taaaagaaat atatattaat atttttgtat aaaaatacct gatatttttt
    54481 tatcgatact ttattgctat acactaaatt aagatagcaa tttatattaa gaaaaaactt
    54541 tttgcatagg ttataatact taaaaggaga ggatagagtt gaaaacaaat acagatgtta
    54601 ttatggagat aattgattat atagaagaaa atcttacaga aaatctaaat ttagataaaa
    54661 tagcttatat tgctaattat tcaaaatacc atttacacag aatgtttaca aatgttgtag
    54721 gatgtactat ccatcaatac atacagagga ggagacttac tgaagcagct agacaactta
    54781 tatttacaga caaatatatt atagacatag ctattacagt aggatattct acacatcaat
    54841 cttttacact agcatttaga aatttatata aagaatctcc acaagtatat agaaataaag
    54901 atgaatttta tcctattcaa cagagatttg ttttaaatct aaatcatcaa attcaaagtg
    54961 aaaaatgtat ttcttatgca aatatgaatt ataaacaaag aagagatgaa atcaattctt
    55021 atctgaactt taatattaat tgtgaggtga aagtagcatg agtttagtgc cacatgtaat
    55081 tgaacaaaca ggtcaagggg aaaggtctta tgatatatat tcaaaattat taaaagatag
    55141 aattatattt ataggagaag aaattaatga tgctattgca agtttagtag tagctcaatt
    55201 actgttttta gaatcagaag acccagattc agatataatt atatatataa atagccctgg
    55261 tggctctgct acatcaggat ttgctatata tgatactatg aattatgtca gatgtgatgt
    55321 atcgactatt tgtattggta tggctgcaag catgagtgct tttttacttg caggaggaac
    55381 acatggaaaa agatgtgctt tacccaattc agagattatg attcatcagc caatgggagg
    55441 agctaaaggt caagcaacag atgtaaaaat agctgtagat aatatattga aaataaaaga
    55501 acgattagat aaaattcttt ctgaaaatac aggtaaatca atagaagata ttaggagaga
    55561 tacagataga gataatttta tgactgcact agaggctaaa gagtacgggc tgattgatta
    55621 tataatgaat aggaatgaat aaagtatcaa agtatcatat taaaatgtct gttttacaaa
    55681 tatatatata atattaaata agagtgtctc aaaatgaact ttttagttta tgggacactc
    55741 ttttttgtat gaaattaaat atggttttat catttcgaca attattacca aatttcaagt
    55801 ttaataaatt ctactataaa aaatacataa aagataaggt tctttagtaa ctttctgtcg
    55861 tcaaaagttt ttataaattt aataacaaaa caagagaaaa taccatatat tacttatgtc
    55921 tgcaatatat agaagacaaa ggagttgtac attatgatta caataagttt gtgcatgatt
    55981 gttaagaatg aggaagaagt tataggcaga tgcttagaat gtgtaaagga tatagtagac
    56041 gaaattataa ttgtagatac cggttctaca gattccacaa aagaaatagt ctcaaaatat
    56101 acagacatgg tgtacgattt cgaatggata gatgatttct ctgctgcaag aaatttttca
    56161 ttttctaaag cttctaaaga ctatactatg tggcttgatg cagatgacat aattttagaa
    56221 gctgatagag aaaaattatt aaagattaaa gaaagcttgg atacttccat tgatattgtt
    56281 atggcaaaat acaatgtatc ctttgatgaa aatggtaacc caactctctc atattataga
    56341 gaacgattgt ttaaaagaag ttctaatcat aaatgggttg accctattca tgaagtagta
    56401 cctctttttg gagaggtatt ttattctgac attgcaatat ctcataaaaa gcttcataga
    56461 caggaccctc taagaaatct tagaattttt gaaaaaatga tttcagaagg aaaaacattg
    56521 gagccaagac accaattcta ttattctaga gagttgtact ataatgctag gtataaagaa
    56581 gcaatagaag gttttactaa gtttttagac tcatcaagag gatggattga ggattgtatc
    56641 agtgcttgca gagatttagc cacatgttat tacttaatta atgatgaaaa aagtgcctta
    56701 tattcattgt ttagaagttt cgaatttgat gagccaagag cagaaatttg ttgtgacata
    56761 ggaaagcata tgtttgatag acaaaaatat aaagaagcca ttttttggta taaggtagca
    56821 ttaacaagag ataagaatga tactgatgga ggctttaagt cagatgattg ttatggatat
    56881 ataccatcta tacaattaag tgtctgttat gacaggttag gagaatcaga taaagctatt
    56941 tattatcatg aaaaaacaaa agaaatcaaa cctaatgata gtgctgtttt gcacaatgaa
    57001 aattatttta gtaaatttaa aaatagttga tgggtcacaa tcatactaaa tttgacatat
    57061 tatatataga gcttaaaagc tcaaatatat caggaggtgc ttttaataat gagtagaaat
    57121 aaatattttg gaccatttga tgataatgat tacaacaatg gctatgataa atatgatgat
    57181 tgtaacaatg gtcgtgatga ttataatagc tgtgattgcc atcattgctg tccaccatca
    57241 tgtgtaggtc caacaggccc aatgggtcca agaggtagaa ccggcccaac aggtccaacg
    57301 ggtccaacag gtccaggtgt aggggcaaca ggcccaacag gaccaaccgg tccgactggc
    57361 ccaacaggaa ataccgggaa tacaggagca acaggattaa gaggtccaac aggagcaaca
    57421 ggggcaacag gcccaacagg agcgacagga gctatagggt ttggagtaac aggtccaaca
    57481 ggcccaacag gagcgacagg agcaacagga gcagatggag taacaggtcc aacaggtcca
    57541 acgggagcaa caggagcaga tggaataaca ggtccaacag gagcaacagg ggcaacagga
    57601 tttggagtaa caggtccaac aggcccaaca ggagcaacag gagtaggagt aacaggagca
    57661 acaggattaa taggtccaac aggagcaaca ggaacacctg gagcgacagg cccaacagga
    57721 gcagtaggag caacaggaat aggaataaca ggtccaacag gtccaacagg agcaacagga
    57781 gcagacggag caacaggagt aacaggtcca acaggggcaa caggggcaac aggagcagat
    57841 ggagtaacag gtccaacagg ggcaacagga gcaacaggat ttggagtgac aggcccaaca
    57901 ggtccaacag gagcaacagg aataggaata acaggagcaa cagggttaat aggtccaacc
    57961 ggagcaacgg gagcaacagg agcaacaggc ccaacaggag taacaggggc aacaggagca
    58021 gcaggactaa taggaccaac cggggcaaca ggagcaaccg gagcagatgg agcaaccgga
    58081 gcgacagggg caacaggagc aacaggtcca acaggagcag atggattagt aggtccaaca
    58141 ggagcaacag gagcaacagg agcagatgga ttagtaggcc caacaggtcc aacaggggca
    58201 acaggagtag gaataacagg agcaacagga gcaacaggag cgacaggtcc aacaggggca
    58261 gatggattag taggtccaac aggagcgacg ggagcaacag gagcagatgg agtagcaggt
    58321 ccaacaggag caacaggagc aacaggaaat acaggagcag atggagcaac aggtccaaca
    58381 ggggcaacag gtccaacagg agcagacgga ttagtaggtc caacaggagc aacaggagca
    58441 acaggattag caggagcaac aggagcaaca ggcccaatag gagcaacagg tccaacagga
    58501 gcagatggag caacaggggc aacaggagca acaggtccaa caggggcaga tggattagta
    58561 ggtccaacag gagcaaccgg agcaaccgga gcgaccggag caacaggtcc aacaggagca
    58621 acaggagcaa caggagcaac aggtccaaca ggtgctagtg caataatacc ttttgcatca
    58681 ggtataccac tatcacttac aactatagct ggaggattag taggtacacc aggttttgtt
    58741 ggctttggta gttcagctcc aggattaagt atagttggtg gagtaataga ccttacaaac
    58801 gcagcaggaa cattgactaa ctttgcattt tcaatgccaa gagctggaac aataacatct
    58861 atttcagcat acttcagtac aacagcagca ctttcacttg ttggctcaac agttacaatt
    58921 acagcaacac tttaccaatc tactgcacca aataactcat ttacagctgt accaggagcg
    58981 acagttacac tagctccacc acttacagga atattatcag ttggttcaat ttctagtgga
    59041 attgtaacag gattaaatat agcagcaaca gcagaaactc gattcttact agtatttact
    59101 gcaacagctt caggtctttc attagttaat actgtagcag gatatgcaag tgcaggaatt
    59161 gcaataaatt agattagata agaaaaggct tctaatatcg tattgatatt aggagctttt
    59221 ttaagaatga ttgatgaaca tagttttaat attaattagt gcgcatatta taaaatacta
    59281 ttatataata taaaataata acattaaatt ggaaatatag aattatctag gggtgatact
    59341 atgaaaaata agattgaaat atcaatgaac aactttgtaa aaaattatca taaatcaaat
    59401 aatataacta caaagtggca agaacttttg tttgcttatg catctgctga agatgatatg
    59461 tttcagaagc taaaaagtgt tgtaggaaca tctcatgcta tgccaaaaga ttttctggaa
    59521 aatggaaaaa ctataattac atattttata ccttttgatg aaagtgtaaa taatagtaat
    59581 attggagggt ttgaatgttc tgaaatatgg gcaagagcgt acatagaaac taatatgttg
    59641 atttcaaatt taaatgattt cttatgtgaa gaactagaga agatgggggc taattcatat
    59701 gctattcctg ggtcgtataa ttttgacagt gaaaaattgg taagtgactg gtcacaaaga
    59761 catgtagcat tcatagcagg tcttggaact tttggtttaa ataatatgct tattacagaa
    59821 aaagggtgtt gtggaagaat aggaagcata atcacaagtt tagaattaga gccaactaaa
    59881 agaccagaaa atgaatactg cttgtataaa cataataata gttgcaaaaa atgtgtcaac
    59941 aaatgcgtga aagaagcttt aaaagaagat ggatttaata ggaataagtg ttatgaaatg
    60001 tgtatgataa atgaaaaaaa atacacttat ataggtggat gtgatatttg tggaaagtgt
    60061 atagttggag ttccttgttc agtaataaac ccatcaagat aatggattta agttgactaa
    60121 taattttaaa gatagtttat aatgaaaaaa ttttagttat caaaataaat ataatataaa
    60181 aactaaataa atgtgtttaa aaaatggata aataatttgt gttgattgac aaccagttat
    60241 actgttgata gacttatatg tatcgctgtg atatataagg agggttgaga ttttatgttt
    60301 gatttaatct ttatttttgc atagcaaggg ctattgcaac tatgaaattt aatatataaa
    60361 tagccctttg ctattcctaa tgaaaaatta ctatttaata ggagagcaaa atgagctata
    60421 aaaaagcaat acatatatta ccagaagaat tacttgaatt aatacaggaa tatgtagatg
    60481 gtgaatgtat ctatattcct agaaaatcta ataataaaaa agaatgggga agtaatacac
    60541 ctactcgtga ggagttgtca attagaaata tgcagattta cgaagactat caagttggat
    60601 atgatttgca atatttatcg gaaaagtatt atttatcttt gaaaagtatc caaagaattg
    60661 tattacaaga aaaaagaaaa gatatctaag gatgagccag tatagtttta taattatact
    60721 ggctcatttt cttttgataa aagtgtttga ggtatagttt cttttaagtg gatgatataa
    60781 tttaagtaat ttcaataact tatacagtag ctaacaaagc tggttgtaaa gtatctaata
    60841 agaagtagta gctttttata tgtttgaaat taaaatttat taaaattagg aggtcttgtt
    60901 ttgaaaaaaa tacttgatta tattccacct tttcataact ttctattaat ttagtaatct
    60961 tatagaaagg aaattgcatt tatgaaaaaa attacttggg aggtggaata tatctcccag
    61021 ttacctgtaa ttaggtattg taaaaaatgt ggaaaaaaga ctgaatatat ttgttcaggt
    61081 ttatttaggg ttaatgctca acataaatat ttggatatat ggttaatata caagtgttca
    61141 aattgtgatt caacatggaa ctcaactata tattctcgtg ttaatccaaa tagattaagt
    61201 acagaaacac tagaacaatt ccatacaaat gatgataatc ttgctaatca atatgctatg
    61261 aatgtagaac ttttacatag aaatggagca gaggttggta ttccagaata taaaatagta
    61321 ggacaagaaa ttgatattaa tgaccttaca caagttcgta ttgtaagtaa gtatccatcc
    61381 caattaaagg tgtcagcatt attacgtgaa aaactaggtt tatctaggaa agcatttgat
    61441 gaaatgttag attgtgggac tattaaaagt atatctggag ttgatttaag gagaagtaaa
    61501 ttaaattatg aaattgtgtt agaggtaggc aagcagaagc aaaagtcttc aaactgatta
    61561 gaataactaa cttaatatat tttcaggaac atcatttaaa aaaatagtga ctttaaaaaa
    61621 tttaaagcca ctatttttta atatgtttta tatgagtatt taaaataaag tgtgtattct
    61681 tcaaatttat agcgataatt aaaacattac tataaaaagg aaggtgcata ctttttattt
    61741 aacaaaaact agatattaaa aacttttcga ggaatatttt attaaaatcc ctaaatatat
    61801 ttaatatata ttaaaaaatt gtattataat gaaaatcaaa atagattgca aatccaaaaa
    61861 gtaatactta atgtccaaaa agctaaaatt aaaatctaaa tagctattta ctacagaagt
    61921 atgttaaaat atgtaaaatg aatagaaatt tatatatttt tattaaaaat atataagaaa
    61981 ggatgtttaa gaattgtgat taatatagga atatgtgatg atgaactaca ttatcgaata
    62041 aaaataaaag atatattaag tgaaatatta agttcatatc ctataaatta taacatatat
    62101 gaattttcag caggagaaga gttactaaat aactatccta aagatttaga catattaatt
    62161 atggacatac aaatgaaaac aataaatggt atggatactg ctaggaagat aagagagttt
    62221 gatcataagt tagaaataat atttgttact tcatttgtag agtttatgca agaaggttat
    62281 gaggtaaaag catacagata tatactaaag ccaataaata aagaaaaaat ttctaaaagt
    62341 gtattacctt gtataaatga gatgatgaaa aagagaaata attacttaac tataaatgta
    62401 aaaaattatg tagaccgaat taaaatagat tctattactt atatagaaac agatagaccg
    62461 aatatattga tttatacaca tgatgatatg tatattacta aaatgagtat atctaaaata
    62521 gaaaagatat taaatgaata tggatttttt agatgtcata atagctatat agttaactta
    62581 aaattagtag aatcaatgag tggaagtact gttatagtag atggaaaaag tattccaata
    62641 agtaaatata gggttaaagg cttgaagctt gcgataacta atatattagg ggacatagta
    62701 tgctaacaaa tttaagtgta ggattttgga atattattaa tttagtttca ggtactatag
    62761 attggattat atttttttta gtaatccact tagttggaaa gaggaaaata tctaatataa
    62821 gatatatatt tacaacaata attataatta tatttatggc atacataaat gtagttaaag
    62881 tgtttccaaa tattaagatt cttctatgta tggtaatagg aatatcattt tatataacat
    62941 catatgaaga taaaatctat aaaagtataa tagttagttt attgttttgg ctaggattaa
    63001 tggttgctga aggttctgca gtaggtctag tagtatttat aaataaatta aataatatta
    63061 acataacaca acatgaaaac ttatttagaa tacaagctat tattatgtca aaggtatttt
    63121 tatttatagg ttttatattg tttaagtatt ttaaattatc tttagatttt aaacctaaag
    63181 atatgatact aataggaata cctatattat ctaatatagt tagcttatta cttatatttg
    63241 aatataattt aagagtcaat acaattacta cagaaaactt atttatattt atatttgcaa
    63301 ttttattg
//
LOCUS       DAGSHT010000004        62771 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGSHT010000004 DAGSHT010000000
VERSION     DAGSHT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.005"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            16..156
                     /locus_tag="MJB72_000203"
     CDS             16..156
                     /locus_tag="MJB72_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261381.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(331..651)
                     /locus_tag="MJB72_000204"
     CDS             complement(331..651)
                     /locus_tag="MJB72_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBY8261382.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(771..1508)
                     /locus_tag="MJB72_000205"
     CDS             complement(771..1508)
                     /locus_tag="MJB72_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBY8261383.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNANIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1723..3546
                     /gene="acd"
                     /locus_tag="MJB72_000206"
     CDS             1723..3546
                     /gene="acd"
                     /locus_tag="MJB72_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBY8261384.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3761..8059
                     /locus_tag="MJB72_000207"
     CDS             3761..8059
                     /locus_tag="MJB72_000207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBY8261385.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8263..8724
                     /locus_tag="MJB72_000208"
     CDS             8263..8724
                     /locus_tag="MJB72_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBY8261386.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8754..9908
                     /gene="nusA"
                     /locus_tag="MJB72_000209"
     CDS             8754..9908
                     /gene="nusA"
                     /locus_tag="MJB72_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBY8261387.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            9915..10196
                     /locus_tag="MJB72_000210"
     CDS             9915..10196
                     /locus_tag="MJB72_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBY8261388.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10186..10497
                     /locus_tag="MJB72_000211"
     CDS             10186..10497
                     /locus_tag="MJB72_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBY8261389.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10519..12459
                     /gene="infB"
                     /locus_tag="MJB72_000212"
     CDS             10519..12459
                     /gene="infB"
                     /locus_tag="MJB72_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBY8261390.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12502..12882
                     /gene="rbfA"
                     /locus_tag="MJB72_000213"
     CDS             12502..12882
                     /gene="rbfA"
                     /locus_tag="MJB72_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBY8261391.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12863..13846
                     /locus_tag="MJB72_000214"
     CDS             12863..13846
                     /locus_tag="MJB72_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBY8261392.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13859..14764
                     /gene="truB"
                     /locus_tag="MJB72_000215"
     CDS             13859..14764
                     /gene="truB"
                     /locus_tag="MJB72_000215"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBY8261393.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            14895..15824
                     /locus_tag="MJB72_000216"
     CDS             14895..15824
                     /locus_tag="MJB72_000216"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBY8261394.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            15928..16185
                     /gene="rpsO"
                     /locus_tag="MJB72_000217"
     CDS             15928..16185
                     /gene="rpsO"
                     /locus_tag="MJB72_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBY8261395.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16425..17270
                     /locus_tag="MJB72_000218"
     CDS             16425..17270
                     /locus_tag="MJB72_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY8261396.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17395..19506
                     /gene="pnp"
                     /locus_tag="MJB72_000219"
     CDS             17395..19506
                     /gene="pnp"
                     /locus_tag="MJB72_000219"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBY8261397.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19772..20515
                     /locus_tag="MJB72_000220"
     CDS             19772..20515
                     /locus_tag="MJB72_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY8261398.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20673..21920
                     /locus_tag="MJB72_000221"
     CDS             20673..21920
                     /locus_tag="MJB72_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY8261399.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            21955..22200
                     /locus_tag="MJB72_000222"
     CDS             21955..22200
                     /locus_tag="MJB72_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBY8261400.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22210..23433
                     /gene="dapG"
                     /locus_tag="MJB72_000223"
     CDS             22210..23433
                     /gene="dapG"
                     /locus_tag="MJB72_000223"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBY8261401.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23546..24280
                     /locus_tag="MJB72_000224"
     CDS             23546..24280
                     /locus_tag="MJB72_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBY8261402.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24403..26814
                     /locus_tag="MJB72_000225"
     CDS             24403..26814
                     /locus_tag="MJB72_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBY8261403.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26817..27875
                     /gene="mnmH"
                     /locus_tag="MJB72_000226"
     CDS             26817..27875
                     /gene="mnmH"
                     /locus_tag="MJB72_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBY8261404.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            27869..29203
                     /gene="rimO"
                     /locus_tag="MJB72_000227"
     CDS             27869..29203
                     /gene="rimO"
                     /locus_tag="MJB72_000227"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBY8261405.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29190..29732
                     /gene="pgsA"
                     /locus_tag="MJB72_000228"
     CDS             29190..29732
                     /gene="pgsA"
                     /locus_tag="MJB72_000228"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBY8261406.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30020..31066
                     /gene="recA"
                     /locus_tag="MJB72_000229"
     CDS             30020..31066
                     /gene="recA"
                     /locus_tag="MJB72_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBY8261407.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31242..32783
                     /gene="rny"
                     /locus_tag="MJB72_000230"
     CDS             31242..32783
                     /gene="rny"
                     /locus_tag="MJB72_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBY8261408.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33097..35433
                     /locus_tag="MJB72_000231"
     CDS             33097..35433
                     /locus_tag="MJB72_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8261409.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            35815..37596
                     /locus_tag="MJB72_000232"
     CDS             35815..37596
                     /locus_tag="MJB72_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBY8261410.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(37678..38208)
                     /gene="lepB"
                     /locus_tag="MJB72_000233"
     CDS             complement(37678..38208)
                     /gene="lepB"
                     /locus_tag="MJB72_000233"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY8261411.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(38248..39072)
                     /locus_tag="MJB72_000234"
     CDS             complement(38248..39072)
                     /locus_tag="MJB72_000234"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBY8261412.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            39329..40237
                     /locus_tag="MJB72_000235"
     CDS             39329..40237
                     /locus_tag="MJB72_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY8261413.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            40381..41826
                     /locus_tag="MJB72_000236"
     CDS             40381..41826
                     /locus_tag="MJB72_000236"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBY8261414.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            42137..42589
                     /locus_tag="MJB72_000237"
     CDS             42137..42589
                     /locus_tag="MJB72_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8261415.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(42924..44120)
                     /locus_tag="MJB72_000238"
     CDS             complement(42924..44120)
                     /locus_tag="MJB72_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY8261416.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            44505..45455
                     /gene="galT"
                     /locus_tag="MJB72_000239"
     CDS             44505..45455
                     /gene="galT"
                     /locus_tag="MJB72_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBY8261417.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            45616..46368
                     /gene="xth"
                     /locus_tag="MJB72_000240"
     CDS             45616..46368
                     /gene="xth"
                     /locus_tag="MJB72_000240"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBY8261418.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(46460..47236)
                     /locus_tag="MJB72_000241"
     CDS             complement(46460..47236)
                     /locus_tag="MJB72_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY8261419.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            47551..49449
                     /locus_tag="MJB72_000242"
     CDS             47551..49449
                     /locus_tag="MJB72_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBY8261420.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(49577..50698)
                     /locus_tag="MJB72_000243"
     CDS             complement(49577..50698)
                     /locus_tag="MJB72_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBY8261421.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            50897..51229
                     /locus_tag="MJB72_000244"
     CDS             50897..51229
                     /locus_tag="MJB72_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY8261422.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            51216..52274
                     /locus_tag="MJB72_000245"
     CDS             51216..52274
                     /locus_tag="MJB72_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBY8261423.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     YEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            52267..53337
                     /locus_tag="MJB72_000246"
     CDS             52267..53337
                     /locus_tag="MJB72_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBY8261424.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            53439..53975
                     /locus_tag="MJB72_000247"
     CDS             53439..53975
                     /locus_tag="MJB72_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY8261425.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            54091..54798
                     /locus_tag="MJB72_000248"
     CDS             54091..54798
                     /locus_tag="MJB72_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY8261426.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            54800..55516
                     /locus_tag="MJB72_000249"
     CDS             54800..55516
                     /locus_tag="MJB72_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBY8261427.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            55518..56276
                     /locus_tag="MJB72_000250"
     CDS             55518..56276
                     /locus_tag="MJB72_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBY8261428.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            56411..57799
                     /locus_tag="MJB72_000251"
     CDS             56411..57799
                     /locus_tag="MJB72_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBY8261429.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(57875..58696)
                     /locus_tag="MJB72_000252"
     CDS             complement(57875..58696)
                     /locus_tag="MJB72_000252"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBY8261430.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            58932..60101
                     /locus_tag="MJB72_000253"
     CDS             58932..60101
                     /locus_tag="MJB72_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBY8261431.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            60284..60484
                     /locus_tag="MJB72_000254"
     CDS             60284..60484
                     /locus_tag="MJB72_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY8261432.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            60905..61333
                     /locus_tag="MJB72_000255"
     CDS             60905..61333
                     /locus_tag="MJB72_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBY8261433.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(61476..62231)
                     /locus_tag="MJB72_000256"
     CDS             complement(61476..62231)
                     /locus_tag="MJB72_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY8261434.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT        24996 a         6916 c        10991 g        19868 t
ORIGIN      
        1 gagagggtgt aacaaatggg aataatagag tcagcatcaa aattggctga gatggttcat
       61 ttattggctg ttgaaaaggg gataactgat atagaagcat gggatgaagc tgtgaaagaa
      121 tacagcaaaa tttatgaaga aagaagaaat gaatagaatg ataaaaaatg aaaacttgat
      181 gataaaaaat aactttattt aaagaaattc aatttttata gaagtattaa aaataatata
      241 aataaaaaga tggtatttca aatgtaagta aaaaatcatt ttgggatacc atccttatta
      301 tttattttaa taatatataa gtaataaatc ttacttatcc atatttttta tgtaacctct
      361 tttttctagc ttttttatat gctccttacg tatttcatca agattaatac catattgttc
      421 ttctagctta aacatcatag taacacatgt ctgcgctaca tccattagtt ccttagcagt
      481 tttctttata atatcaactt catctaaatc attattttct ccacttagat ttctgaattt
      541 acctatacat tcagctaatt caccttgttc ttcacccatt ttgataaatg tagattctag
      601 tgttgggtct aaattattaa gctctggtaa agatatagtt tttagtttca taaaagctcc
      661 tttcaagtta ttcgtattta tactaaaaca tcataacaaa aataatacta aaaaaaaact
      721 ttaaattcaa cataaaaact tacatatata ataaaaatcg tgaaataaat ctatatttgt
      781 cttaatacag cctcatataa aacttctcca ataggtttat ttattacaag agaagctgac
      841 ttatcataga ctgtatttgc tttattaata agagcaatat aatcaccttt gtaataatct
      901 ataaaacttg ctgctgggta cacagctaat gatgtacctc ctataatcaa taaatcagca
      961 ttacttatag cagaaattgt tttagtaatg acatcactgt caagagcttc ctcataaagt
     1021 actacatctg gttttactat agaaccacag ttgtcacagt aaggaatatt acctcccaga
     1081 tttaacatag attctaagtc aaaaaactta ccacatttag tacaataatt tctatgtaca
     1141 gaaccatgaa gttctagcac atttttactt ccagccattt gatgaagtcc atcaatattt
     1201 tgagttatga cagctttaag cttacccatt tcttcgagtt ttgctaaagc tatattagca
     1261 ttattgggtt ttgcattagg atatattaat ttatccttat aaaattcaaa aaattcttct
     1321 ggatatctaa caaaaaaagt atgtgaaact aattgttcag cagtaaattg tttattgagt
     1381 ttttggctaa ataatccagt agaacttcta aagtctggaa tattagattc agttgaaaca
     1441 cctgctccac caaaaaatac aatattattg tgattagcta ttaagtcttt taagctattt
     1501 gcatccatag ttacacctcc aaattattat atataaattg tactacatat taggtcgttt
     1561 atgttttatt aatttaaata aatatatagt ttaataagtt aaataaaggg aatataaata
     1621 aaatgtatac agagtataaa tagacaatat ttactaaaat aacaagttgt cgtataatta
     1681 tattaaaatt gttttagtgt ataagaaggg cgggggaagt acgtgaagaa aaaagcagct
     1741 ttagcaacat tggctatgtt acccttaggt gtggtaaatg cacatgctga tggagatata
     1801 ggtatagtga ctataaatta tttaaatgta agaaatgaac caactgccga aagtagcata
     1861 gcctttgttg ctaaaaaaga tgataaagtt ttgattaaag actcttctaa tggatggtat
     1921 aaaataaaag ctgaatctgg acaagaaggt tgggcttcat caaaatatat agcaaaatca
     1981 aatagtgact ctttaagaac gtctacaaat aaagaaaaac aagttatttc aaatagttta
     2041 aatatgagaa atggagcagg tacaagttac agagtaataa ctgtactaaa aaaaggtcaa
     2101 aaagtagaag taatatcaga gagtaatgga tggtctaaaa tcaaatatga tggaagacta
     2161 ggatatgtat ctagctctta tttaggagac gtttcaaact caactaataa atcaaaaact
     2221 aaacaagtga atacaacttc actaaatgta aggagtggac caaatacaag ttatggtttg
     2281 ttaggcaagt tgccaaaagg aagtaaagta gaagtaatat cagaaagtaa tggatggtca
     2341 aaaataaagt ataatggaaa agatgcctat gtatctagta tgtacttatc ggatgtaagt
     2401 caaagtaatt cagacaattc tagtcaaagc aatgataaaa agaatactga taaggttgta
     2461 aatacagctt ctttaaatgt aagaagtgga ccaggttcta catatagtaa gttgggaaaa
     2521 gtttataaag gaagtaaagt aactgtacta tcagaaagta gtggatgggc taagattaat
     2581 tttaacaata aagaagcatt tgtagtaggc aattatttat ctacttcagc agatacttca
     2641 aataataact caaatagtaa ctctgataac agttctaata gtaatggtaa taattcttca
     2701 tcatcaggtc aagtaaatgg tatgtcaggt ataagtgggg ctaaaattga ttataaatct
     2761 ttgagttata ctttagaatc tcatataagt aaacaagttg agaaagcagc atcaggagga
     2821 aatgtgatag ctccaagtaa taggaaaagt actccaagtc ctgaatttag tactttttca
     2881 gctcaaagaa caagctcatt tgtaaatgca agttcaagtg atatagaata ttatttaaat
     2941 cctaagaatt ttacaaatac tactaaaggt atgatgcagt ttttaaagat taacagttat
     3001 agagatggta tttcagaatc aagtcttaac tcgtatctta atggtttatc ttctagtgta
     3061 tttaaaaatc agggagcagc ttttataaat gctgccaaga agtataatat agatgttgta
     3121 tatctagtat ctcatgctat gtgggaaact gcttatggta agtctacact tgcccaaggt
     3181 caaacattaa catcttataa gggacaacct cttagtaagc cagttaaagt atacaatttt
     3241 tttggtatag gagcaataga taaaagcgct aatgtttcag gtgcagaagc agcgtattcc
     3301 aatggctgga ctagtgtaga agctacaata gatggctctg caaagtggat atcacaaaat
     3361 tacgtaaata gttctaagta caatcagaat actatctaca agatgaagtg gaattatgat
     3421 tatacatggc atcagtatgc aactgatgta aactgggcta atggaatttc tggaattatg
     3481 gaaaatttaa ttggtcttta tggaggagga agtagtttgg tttttgaagt tcctcaatat
     3541 aagtaataca taaataaaaa atatagacta tgggtagttt tcattttatt taaattatct
     3601 atagtctttt tgtatataat ttataatctg taaattcaat gttaacaaaa cttttatgtt
     3661 ataatattaa ttctaagtaa atataagtga gtaataggta gaaatagata aaaagtggta
     3721 ataatatata taaagataat tttgagaaag gaggatatat atggaaagta ttaaggaata
     3781 tttggataag ttagaaatta ataatagtgg tcttggaaag caacttaaag aagtttatat
     3841 aaatagagta gtttatttta aggaagataa gatagtttat ttttatctaa cttctaaaga
     3901 tattgtttct catgaacttt tagataaatt taaagaagaa ttgatgtata aactcgatta
     3961 ttttaaagat atgaggatga agataagatt tactggtctt gaaagaaaat ccaataaaga
     4021 tgttataaaa aaatattgga ataatatatt gtatatatta aagtatcttt gtccatctat
     4081 tgcagggtgg tataaacaag tagaatttct ttgtttagaa gaagaattaa aaataaaact
     4141 tccaaaaggt atattttatg agcgattaat gaaaaaaaat gtaacttatg tacttaaaac
     4201 cgtgcttagc gaagagttgg gattagattt aaatataaca atagaaaaag ctgttgatga
     4261 aaaggtaaat aaagaaagac tcataagaat caatgataga gaaatggaag aaaaaattag
     4321 ggctttagaa attggaaaag ttaataactg tgaagaaaat gaagaggaaa gctatgtcat
     4381 taagtctgat gtagatgaaa atttaatcta tggagataat gcaaatgcta tgattgaaaa
     4441 tatagttgaa ttaaatgcat cttctggaac agttgcagtt gttggagata tatttgatgt
     4501 tgacactaaa gaacttaaaa acggaaaaat acttatgata gcttcaatta cagattatac
     4561 tagctcaata agctgtaaac tttttttaac agatacaaat aaggatggag tacttgaaaa
     4621 tgttaaaaaa ggagcatact taaaaatcaa gggtgacata gtttatgata catatcaaag
     4681 agaaattagt atgatgatta gtgggataag aaaagaaact agaatagaga gagaagataa
     4741 atcagaggaa aaaagagtag aactccatgc acatacacaa atgtcttcta tggatgctat
     4801 ttgttctgtt aaaaagcttg tagagcgtgc agccaaatgg ggtcattctg ctattgctat
     4861 aacagaccat ggtgttgtac aaggatttcc agatgctatg aatgcaggta agtcaaataa
     4921 tataaaaata ctttatggag tagaagggta cttggttgaa gatgattctc tcattataga
     4981 ggatgcaaat gataaagaac tttcacagac atttgttgta tttgacatag agacaactgg
     5041 tttttctaat acaaatgata aaataactga gattggtgct gtaaaaatag aaaactttaa
     5101 gattgtagat agatttagtg agttaataaa tcctgaaaaa gatatttctt ataagataca
     5161 ggaattgaca ggaataacta atgaattaat taaagacaag ccaaccattg aagaagttct
     5221 tcctaagttt atggaatttg ttggagatag tgtattagta gcacataatg cagagtttga
     5281 tactggattt atatcgcaaa aatgtaggga acaaggcttg ttatataata acaaaaaagt
     5341 agatacactt atgttagcaa gagtaatgtt gccaaactta aaaagacata gattaaatgt
     5401 cgttgcaaaa gaacttggta ttcctctttt aaatcaccat agagcagtgg atgatgctga
     5461 agctacagca cttatattta ataagttttt gcagatgctt acagaaaaag gtgcaaaaac
     5521 tctaagtgat gtaaacaata tacttggaaa gattgattac acaaagctta gcacaaatca
     5581 tataaccttg atagcaaaaa attctatagg tataaaaaat ttatataaaa taatttcaga
     5641 tgcacatgta aatcattttt ttagagcacc aagaatcctt agaagtgtat taaatgaata
     5701 taaagaagga ttaataattg gttcggcttg tgaagctggg gtggtttttc aagctgtaaa
     5761 gaaaaacgtg tctgatgagg aaatgaaaaa aataatagat ttatacgatt atattgaagt
     5821 aatgcctata gataataata gatttatgat tgataaagga gaagtaaagg acgaagaaga
     5881 gttaagggag ttgaatagga aattaattga tacagccaaa aaatttgaca aaattccagt
     5941 agctactggg gatgttcatt ttttagacaa acatgaagct gtcttaagaa aagttcttaa
     6001 atattctcaa ggttttaaag tagatgaaga agaaacttat ttacatttta gaacaacaga
     6061 tgaaatgttg gaagagttta gttatcttgg agaagaattg gcatatgaag ttgtagttga
     6121 aaatagtaat ctgattgccg atatggttga agatataaaa cctataccag atgatactta
     6181 tcctccaatt atagagggct cagatgtgga gttaagagaa atgtgttaca aaaaagccaa
     6241 acgaatctat ggagacccaa taccagaaat agttcaaaaa agactggata gagaattaaa
     6301 ttcaataata agcaatgggt atgcagttat gtatattata gctcaaaaac tggttgcaaa
     6361 atctctttca gatggatatt tagttggttc aagaggctca gttggctcat cttttgcagc
     6421 tactatgagt gatataacag aagttaatcc tcttccagca cattatattt gtgaaaacga
     6481 agactgtaaa tattcattct tttatgaaat aggtgaatgg ggctcaggtg ttgatttacc
     6541 agacaaggat tgtccaaagt gtggaagaaa acttgctaaa aacggtcatg atattccatt
     6601 tgaggttttc ttaggttttg aaggtgataa agaaccagat atagacttaa atttctcagg
     6661 aaattatcag ccaacaattc ataaatacac agaagaacta tttggagagg ggtatgtata
     6721 tagggctggg actataggta ctgtagcaga aaaaacagct tttggatacg caagaaaata
     6781 tgtggaagaa aataatataa gtgtaccaaa tgcagaagta cttaggcttt caaatggatg
     6841 tactggagta aaaagaacct caggacaaca tcctggtgga gttatggtta taccagacta
     6901 taaagatgta tacgatttta cacctataca gtatccagca aatgatactt cttgtggtgt
     6961 tataactact cactttgatt atcattcaat aagtggtaga atacttaaac ttgatatact
     7021 tggacacgac ggtcctacca ttataaggat gcttgaagat atgactggaa taaatataac
     7081 agaaattcct ttagatgata aagagactat gtctctattt acatctacag aagcattagg
     7141 tgttacacca gaagaaataa actgtcctat aggttgcctt gctgtaccag agtttggaac
     7201 taagtttgta cgacaaatgt tacttgacac aaaacctact acatttgcag aacttgtacg
     7261 tatatctggt ctctctcatg ggacagatgt ttgggtaaat aatgcacaag acttagttag
     7321 agaagatata gttggattaa aagatgttat atctacacga gatgacataa tgaactattt
     7381 aatatttaaa ggattacctc ccaaaatgag ctttacaata atggagagtg taagaaaagg
     7441 taaaggttta aaaccagaac acattgaaga aatgaaaaaa aataatgttc cagaatggta
     7501 tataggctca tgtaaaaaga ttaagtacat gttcccaaaa gcacatgctg ttgcttatgt
     7561 aatgacatca tttagaattg catattgtaa agttcactat ccagaagcat tttatgcgac
     7621 ttattttacg acaaaagttg aggattttga tgcagattta attgttaaag gattagatgc
     7681 tataaaatca aaaataaatg aaattgagtc actgggaaat gatgcaacca caaaagaaaa
     7741 aggtatgttg accgttttag aagttgctct tgaaatgtat gctagaggaa taaaattatt
     7801 accagttgat atatataaat cagatgcaac tgagtttata gttgtaggag aaaaaacatt
     7861 acttccacca atggcagcaa tacaaggtct tggaggaaat gcggctataa acatccaaaa
     7921 tgaaagaaaa aatggtgaat ttatatcaaa agaagatttg agaaagagaa caaaaatatc
     7981 aaaaacggta atagaaactc ttactaatca tggttcactt gagaatatga gtgagagaaa
     8041 tcaattgtca ttgttttaag ttagcccaaa aagttgcaaa taaaagaatt ttatgatatc
     8101 atagtataga aaatagtata ctttgcagaa actaagagtg agacagctgt tctcactctt
     8161 ttattttaaa tatattatta aaaagctggt ttatttttat taaatatatt ttatttaaga
     8221 ttatataaac tggtcattta caattgaata ggaggaactt taatgaaaaa aaatttagaa
     8281 gctacaattg aggagattgt aactaaaata acggatgaac atggatttga aatggttgat
     8341 gttgaatatg taaaagaagc aggagaatac tacttaagag tatacataga taaagaagaa
     8401 ggaatatctt taaatgaatg tgaattagtt agtagagaat taagtccaat actagatgaa
     8461 aaagacccta taaaagaaaa ttacttttta gaggtatctt ctccagggct tgatagagca
     8521 ttaaaaaaag acagagattt tgttagatat caaggtagag atgtagattt aaaattatat
     8581 aaaccattaa atggatgtaa gcaatttgaa ggtgaacttg taggattaac agaagataat
     8641 aatataaaaa tcattgcaaa tggtaaggaa atggaattta atagaaaaga tgttgctatt
     8701 gtcagattag caataaagtt ttagtggtat attaaatagg gaggatataa aaaatgaatc
     8761 atgaatttat ggaagcatta gatgaattag tagttgacag aggtatagat aaagaaatac
     8821 ttatagatac tatagaacaa gctcttttaa ctgcatacaa gaaaaacttt ggttcagcac
     8881 aaaatgttag agttgaattt gatagagaca agggagatat aaaagtattt tctcaaagag
     8941 ttgttgttga tgaatcagat ttatatgata ctttcttaga aatagagtta gcagatgcta
     9001 gagagataaa ccctaattat gaattaggtg atattataga acatgaagtt acacctaaaa
     9061 actttggaag aattgcagct caaacagcaa agcaagtagt tgttcaaaga ataagggaag
     9121 ctgagagaga aatagtgtat aatgaattta tagaaaaaga aaatgaaata gttacaggag
     9181 aggttgctag agcaaataaa aatgttgttt atgtaaatct tggaagaata gaaggtataa
     9241 tgactcaaag tgaacaaata ccatttgaga attataaagc aggtcaaaaa ataaaattct
     9301 atatacttga agttaaaaag actaacaaag gacctcaaat agttgtatca agaagtcatc
     9361 cgggtctagt aaagagattg tttgaagaag aagttcctga aatatatgaa ggaatagttc
     9421 aaataaaatc tgtagcaaga gaagcagggt caagaactaa aatggcagta agatctatag
     9481 atgaaaaaat agacccaatt ggagcttgtg ttggaccaaa aggacttaga gtaaaaaata
     9541 tagtggatga acttggcgat gagaaaatag atataataaa atatagtgaa gacccagcag
     9601 agtttatatc agcagcactt agcccatcaa aggttgttag agtagatata gatgaagatg
     9661 aaaagtctgc cttagtaatc gttccagatt atcagttatc gcttgctata ggtaaagaag
     9721 gtcaaaatgc aagactagca gcaaaattga ctaactggaa aatagatatt aagagcgaat
     9781 ctcaagcaga acaagaaatg ttaaatcttg aaaattccaa agaaatagaa gctgaggatg
     9841 ttgaagctga atttatcgac caagaagagt tagatacttc agaggataat tttagtgaag
     9901 aagagtaagg agggatgatt ttgaaaaaag taaaaaaagt gcctcaaaga aaatgcatag
     9961 catgtcaaga tagagactct aaaaaagagc ttataagaat agttaaaaat aaagaaggaa
    10021 aaatattttt agacccaaca ggaagagcca atggaagagg agcatatgta tgtaaaagct
    10081 ctgaatgtct aaaaaaagct ataaagagta aagcattaaa taaagctttt aaaatagatg
    10141 ttcctgatga agtctatgat aatctactta tggagttaga agaatatgaa aaataataaa
    10201 gaaaaagtac tttcgttctt agggctcgca actcgtgcag ggaaaatagt gtctggtgat
    10261 gattcaactt tgcttgacct taaaaaagga aaagtaaatt tgattttaat cgcagaagat
    10321 gcatcaaata atactaaaaa actttttaaa gataaatcaa cttttagaaa tattccatat
    10381 ctattttttt caacaaaaga ggaaatagga tttgcaatag gaaaatctcc aagagcagtt
    10441 gtaggaataa aagatgaaaa tttttctaaa aagataattg aactaataga aatttaaaat
    10501 aaaatagggg gtgatcttgt gtcaaaaaca agagtatacc aaatagcaga agaattaaat
    10561 atatcaaatg aagagcttat aaataagtta gctgaattag atataaatgt aactgacaaa
    10621 gattcagtat tagaaggtga agagttagaa ttagcacttg aaatgttagg tgaagattta
    10681 tctcaagaaa atggaaatgt tatagaaata gatggaaagt tgacagtaca agtattagca
    10741 acgaaattag ataaatctcc atcagaaata attatgaagc ttatgaaaat gggaactatg
    10801 gcaacaataa atcaagagat aagttttgag attgctgctt tggctgccaa agactatgga
    10861 tttgaattaa cagtagctga aagtgatgat acagaagctt tagaaataga agctttaatg
    10921 gaaattgaag aagataaaga agaggattta aaacctagac caccagttgt tacagttatg
    10981 ggacatgttg accatggtaa gacatcttta ctagatgcaa taagaaaaac tgatgtgatt
    11041 tcaggtgaag ctggaggaat aactcagcat ataggtgctt cagaagttaa aatcaatgga
    11101 cacaaaatag tatttttaga tactccagga catgaggctt tcacatcaat gagagcaaga
    11161 ggagcacaag ttacagatat agcaatatta gtagttgcag cagatgatgg tataatgcct
    11221 caaacagttg aagctataaa ccatgctaaa gcagcaggtg tacctttaat agttgctatt
    11281 aataaaatag ataaaccagg tgcaaaccca gataaggtta aacaggaatt agcagaccaa
    11341 ggattattag ttgaagactg gggtggagaa gtaatagcag ttccagtatc agctaagaaa
    11401 aaagaaggaa tagatacact tttagaaatg gtattattgg ttgctgaaat ggaagagtta
    11461 agagctaatc caaataaaag agcagtagga actgttatag aagctgagct tgataaagga
    11521 agagggccag ttgcaacagt acttgttcaa ggtggtactt taacagtagg tgacccaatc
    11581 gttgcaggtg ttgcatgtgg taaagttaga gcaatgataa atgcaaaagg taagagagta
    11641 aaaacagcag gaccatctac agcagtcgag atattaggtc tatcagaagt tccacaagga
    11701 ggagaccaat ttgtcgaagt tccaactgat aagattgcta gaagtgtagc agcaagaaga
    11761 caacaaatag ttagagatga aatgttaaaa tcaactcaaa gattatcact agatgctcta
    11821 tttagtcaaa tgagtgaagg ttctataaaa gaccttaata tagttataaa agcagatgta
    11881 caaggttcag tacaggcagt taaacaatct ttagaaaaat tatcaaatga agaagttcaa
    11941 gttaaagtaa tacatggtgg tgttggagct gtaacagaat cagacatatt acttgcagca
    12001 gcttctaacg caataataat aggattcaat gtaagaccag ttccaggtgc agaatctcta
    12061 ggagaaaaag aaaatgttga tataagaaca tatactataa tatacaaggc aatagaagat
    12121 atacaagcgg ctatgactgg tatgttagac cctgaatatg ttgatgaaga aacaggaaaa
    12181 gctgaaataa gagaaatata taaaatatct ggtgttggaa cagtagctgg atgttatgtg
    12241 actaatggta agatatttag aaattgtaaa gtaagattag ttagagatag cataataata
    12301 catgaaggtg agcttgcagc acttaagaga tttaaagatg atgtaaaaga agttaattct
    12361 ggatacgaat gtggtatgag ttttgtaaat tataacgaca taaaagaagg cgatatagta
    12421 gaagcttata taactaaaga agtagaaaga aaattataaa ttcgtatcaa agaaaatcag
    12481 ttagtaaaga aggtcgatgg tatggcatca tataatagaa caagaaggat agcagaagaa
    12541 ataagaaaag tcgtaagcac tatgcttata aatggagtaa aagaccctag aataacatct
    12601 atggtgagtg taactgatgt agaagttaca aatgacctaa gatatgctta tgtatatgta
    12661 agcattctag gtggagatga agaatccaca ctaacaggtc ttaaaagtgc tggaggatat
    12721 ataagaagag aagtaggaaa aaatataaaa cttagatata ttcctgaaat agtatttaaa
    12781 cttgatgatt caatagaaaa aggaatgtat atggatagcc ttataaaaag agttaatgaa
    12841 aaaaatgctc aacaaaatga ggatgaaaat tatgatgaat aatatggata gtattataaa
    12901 ctatatttat gagggtgact attttatagt tacctctcat atcagtccag atggagacaa
    12961 tattggttct acactttcaa tgtattatac attgaagaat ttaggaaaaa atgtatatta
    13021 tgtacttgat gatgaagctc cattaaattt aaggtttttg gttgaagatg taaaaatcta
    13081 taagtctaat gaatttaata tgaagaacta ttctctaatt gcacttgatt gtggggataa
    13141 gatgagaata tgtgtgagtg atgaaatcaa aaataatgca tcaaaattaa tctgtattga
    13201 ccatcatgct agtaatgatt catatggaga tttaaattat gtagatattg atgcatcttc
    13261 tacatgtgaa ttagtttata atttattagt tagatttcag caaataaaag atattaatat
    13321 tataaatgaa gatatagcaa catgtttata tacaggtctt gtaactgata ctggaaactt
    13381 ttcgtattca aatgtacatg caagtagctt tgaaatggca aaaaatttac ttgttttagg
    13441 tgcacaaaaa aatactataa tacaaaatat atatcaaagc aattcttctg gttattacaa
    13501 attattagga gaagctctaa agggtttgga gatatttgat tcaaaggtat ctagtatagt
    13561 tttaactcaa gacatgatga atagaaataa tattagcttt aatgatgttg atggaataac
    13621 aacatataca agagacataa aaggtataga agttggaatt ttatttaaag agaaaaaaca
    13681 aaatgaaatt aaagtgagtt ttaggtcaaa aaattatgtt gatgtaagtg aaatagccaa
    13741 gttatttggt ggtggtggtc atgtaagggc tgctggatgt actattagag atagtattga
    13801 taatgcaaag aaaatggtac tagaggcagt gttaaaatca atttaaggtg acagtaaaat
    13861 gaataagatt ataagtatat tgaaacccac aggtatgaca tctcatgatg ttgtaagtag
    13921 agtgagaaag attttaaata taaaaaaagt tggtcataca ggtactcttg acccagatgc
    13981 ttcaggtgtt ttaccaatct gtatagggaa agctacaaaa gtatgtgaag ttattttaaa
    14041 caaagataaa tcgtatatat gtgagcttac tttaggtata agtacagata cttatgatgc
    14101 ttcaggtgaa attttaaaaa aagttgatga ttttaaattt agtaatgaag acatagaaag
    14161 agcttttgac acacaaagag gagagataaa tcaacttcct ccaatttatt cagctttaaa
    14221 ggtaaatgga aagagaatgt gtgatttagt aagaagtggt agacaatctg aaattacttt
    14281 aaaaacaaga agagtaaata taaaagacat aaaaatacta agtataaaag gtaataaagt
    14341 aatgttttat gttgagtgtt ctaaagggac ttatgtgaga agtatttgcc atgatatagg
    14401 agaatatcta ggttgtggag ctcatatgtc gtttttgaat agaacatctt ctggtaaatt
    14461 tgacttagat aattcaataa cattagaaga attagaatta ttttatgaaa ataaaacttt
    14521 agacaaatac ttatatgata tagattatgt ccttgattcg tttaattatg ttgtattaaa
    14581 ccctaatgct ataaaatatt atagtaatgg tggctcaata gatgataaaa ggtttttaaa
    14641 gaataatttc gataaagatg atgaatttgt aagagtatac agtacagata atttcttagg
    14701 attaggaaag ttatcaaaac acaataatac aataagtgtt aaaagtgata agatgttcat
    14761 ataaccaatg tatttaaaaa aacaacaaaa gagatagtac tataataact tctatagtct
    14821 atctctttta cttatgctat aatagtttgt gaataagtta agagtaaatt atattttagc
    14881 ggggaacatt tattatggtg attataaaat ctataaaaga aatagcaaac ataaaagaaa
    14941 gtgtcattac cataggaaat tttgatggtg tacacaaagg acatcaagtt cttataggaa
    15001 aaacagttga acatgcaaaa aaagaaaata tacagagtat tgtatttaca tttgcaaatc
    15061 atcctgtaaa ttactttaga ccaaactcaa caaaaaatat tataagcaat gatgataaaa
    15121 taaaattatt agaagatttg ggaatagata tagttgtaat cataccattt gatgagtata
    15181 tgactaaaat acctgcaaag gatttcgtag atgagatact agtaaaaaaa ttaaaagcta
    15241 aaaagatagt aattggacat gactttacat ttgcaagggc aaaagaaggc aatgttaatc
    15301 tattaaaaag cttagaacat gaatttggtt ttgaggtaga agtaataaaa cccatcaaaa
    15361 tcaatgatgt aagagtgagt agtacttata ttagaagttt agtatcccag ggtgatatgg
    15421 ctaatgtcaa ggaatatttg ggtagaaatt acaagttaga aggttgtgtt atacattcta
    15481 aacaccttgg aagaactata ggattcccaa cggcaaatat tgaccttaag aataatatgc
    15541 tcgttccaaa gagaggtatt tatgcttcta ttgtacatat tggagatgag acttattttg
    15601 gtgctacaaa tgtaggctat aacccaacag ttaatggaaa aactttgtct attgaaacaa
    15661 atattttaga gtttgataga gatatttatg gggaaaatat aattgttgaa tttttggaaa
    15721 gaataagaga tgaaagaaag tttaattcaa tagatgactt aaaaaatcaa ttgtataatg
    15781 atacaaatta tgtgtatgaa aactatgttt gcaaaaacaa gtaatgtatg ataaaatgat
    15841 taaggaaaat aaccattact cggcatttga atactccaat caatgcttag taataggcgt
    15901 ttctaaaaaa atacggaggt aatctaaatg ataaacaaat cagaaataat aaaagaatat
    15961 ggaagagctg aaggggatac tggttctcca gaagtacaaa tagctttatt aacagctaga
    16021 ataaacgagt taaatggaca tttaaaaact cataagaaag accaccattc aagaagagga
    16081 ctattaaaaa tggttggtag aagaagaaac ttattagctt acttaaagga aaaagatctt
    16141 gaaggatata gagcattaat agctaaatta ggattaagaa agtaatttca ttctttataa
    16201 aaaagcaggt atagtgtata cctgcttttt tatagttaat tcattataaa gtaaaatagt
    16261 taataataaa agtagtttat atgttataaa aaattataaa atgaataata ttgggtaaga
    16321 atattaaaat agtatacaaa tacataatca atttaaaagg aaagacagga taaatataga
    16381 aattttataa caggataatt agttggaata aggaggatat tattttgaaa ataaagttag
    16441 tatgcgatag tttgtgcgat attccagatg aaatatcaga aaaagattat atagagatag
    16501 ttccacttac agttattttt aatgataggg aatatataga aggagtagat ataaagaaag
    16561 aagagtttta taaaaaagta aaagaaatca agcaaatacc taaaacttca caagctacat
    16621 atatggagtt taaagaagtg tttgataagt tcataacaga aggttatcat ataatctgta
    16681 tgacaggggc tgcaaacgca tcaggaactt ttcaaagtgc tatgatagct aaaaatgatg
    16741 taaatgaaaa tgaaaaaata catatatttg atactagaaa tttatctcta ggtagtggac
    16801 aatatgttat aaaggcatgt gaacttcttg aagaaggact gggatttgaa gaaattatag
    16861 atgaattaga aaatactcgc agtagtgtaa gattgctttt tgcaccatat acacttgatt
    16921 ttttaaagca gagtggaaga gttccagttg caactgcttt gataggaaat atgttaaata
    16981 taaagcctat atttttcttt gataatgggg aagctaagtt ggtaaataag gttagaggaa
    17041 taaagaatat agcaagtaaa ttggtcgata taattttaga aatgaatgaa gggtctttag
    17101 aaggaaaaat cgtcacaata ggttgtggag ataatttaca cgattgtgaa atattaaaag
    17161 atgaagtaaa taaaagaata aaagcaagaa gagtattatt tactcgagga ggagtttcta
    17221 tttgctctca tacagggcca gatatattgg ctataagttg ttctaattag tagtaatata
    17281 aggtttttgc atatatatgt gaagataatt ttatattgtt aatttatagt atttttgata
    17341 atatagtaaa tagagtgata tacgtgtttt ttaataagag gaggacaagc attaatgttt
    17401 gaacataaaa tatttaaaat ggattttgca ggaagagaac tttctgtaga aataggaaag
    17461 atttgtgaaa tggctagtgg tagttgtata gttcgataca gtgatagtat ggtaatggta
    17521 aatacaacta aatcagccaa acctagagac ggaatagact tttttccttt aagtgtggat
    17581 tatgaagaaa aattatactc tgtaggtaag atacctggag gttttttaaa gagagaaggc
    17641 aagccttcag agaaagctat acttacatca agattgatag atagaccaat aagaccttta
    17701 ttcccaaaag gatttagaaa tgatgttcag gtagttgcaa cagttctatc agtagaccaa
    17761 gattgtacac cagatatagt agcaatgata ggttcttcaa tagccctttc tatatcagat
    17821 ataccattta atggaccaac aggttcagta tgtgttggcc ttgtagatgg tgcatttgtt
    17881 gtaaatccaa acgcagagca aagagaaaaa agttcaatgc atctagttgt ttcaggtaca
    17941 aaggaagcaa taatgatggt tgaagccgga gctgatgaag ttccagacga ggtaatgctt
    18001 gatgctattt tatttgctca ccaagaaata aaaaaaatag ttgagtttat agaaggtata
    18061 gttgcagaag ttggaaaaga aaaaatgcct gttgaattat accatgcagg agaagaaata
    18121 actcaattgg ttcgcgaatt tgcaactgat aagatgaaaa aagcagttca gacttttgaa
    18181 aagttagaga gaatggaaaa tatggataga gttaaagaag aaactttagc tcactttgaa
    18241 gaaactttag aagattttga agattttgtt ggagatatag aagaagtatt acaagatata
    18301 ataaaagaag aagtaagaaa acttatagta catgaaaatg taagaccaga taatagaaaa
    18361 cttgaagaaa taagacctat atggtgtgaa actggcatga tacctagagc tcatggttca
    18421 gccatattta ctagaggaca aactcaagta cttaatgttg ctactttagg tgctttagga
    18481 gatgttcaaa aactagatgg gttagatgaa gaagaaaaca agagatatat gcatcattat
    18541 aatttccctg catatagtgt tggagaagct agaccatcaa gaggaccagg aagaagagaa
    18601 ataggacatg gtgctttagc agaaagagct ttattaccag taataccatc tcaagaagaa
    18661 tttccttatg ctatcagatt agtttctgaa gtattaagtt caaatggttc tacatctcaa
    18721 gcaagtgtat gtggctcaac attatcttta cttgatgcag gtgtacctat aaaagatatg
    18781 gttgcaggta tagcaatggg acttataaag catgatggta aggttgcagt gctttcagat
    18841 atacaaggta tggaagacca tttaggcgat atggacttta aagtagctgg aacagagtat
    18901 ggtataacag caatacaaat ggatataaaa attgatggta tagataaaga aattctacaa
    18961 cgtgctttaa aacaagctaa agagggtaga atccatatac taggagagat gagaaaaact
    19021 atatctcagc caaaaccaga actttcacca tatgcaccta agattgttaa aatgcaaata
    19081 aatcctgaca agataaaaga tgtaatagga cctggtggaa agataataac taagataata
    19141 gacgaaactg gagttaagat agatattgaa caaactgggg aagtatttat atcaggtatt
    19201 gagattgata tgattaagaa agctcaagaa cttataaata atatagtagt tgaaccagaa
    19261 gtaggaaaaa cttataaagg taaggtaagt agaataatga actttggagc atttgttgag
    19321 atacttccag gaaaagaagg attacttcat atatctcata tagctcatga aagagttgcc
    19381 aaagttgaag atgtattgaa tataggggat gaagttgaag ttaaagttac agagattgat
    19441 gaaaaaggaa gagttaattt atcaagaaaa gttcttttac caaaaccaga acataaaaat
    19501 aaataactta ttatataatt aaaagcaaag tcttaggatt ttgctttttt aaatacaaaa
    19561 aagtagataa ttttaaaatg atagttatgg atggcttata gttctttttc tgtcaggctt
    19621 tactcttata aagaagctat ttaaaagctg attaatagag agctttattg atatatatag
    19681 aatcacttaa gttatattta gtgcataaac agagcttatt tttaatagta ttaaataaga
    19741 aacaatttgt acataattag ggggagtttt aatgataatg atggtactta ataagagtaa
    19801 gctcaaaaaa atcataatac taattttagt agtgttatta gtattatcag gaggggtaat
    19861 ggtattaaag ttatttaaca acaagccagt atttaattta tttaatggtg tggatttacc
    19921 atatgaaaga ggtacaaagg ataaaagtgg atatgttgca ttaacctgta atatagacct
    19981 tggatgggaa acagagtacg tagaatccat tttagaaaca cttaaaaaag aaaatgtaaa
    20041 gattacattt aatgtaactg gaaaatgggc agaaaagaat aaggatgaat tattaaagat
    20101 aaaaaaacaa ggacatgaaa taggtaatca tggatacaaa catttagatt attctacatt
    20161 atcatatgaa gacaattatg agcaaataga gacctctaaa aagataattg aagaaattat
    20221 aggtgaaaaa actaaatttt ttcaagcacc agcaggttct tttggtccag aaacagtaaa
    20281 ggctgctaaa gcattagggt atacttctat caaatgggat gccgatacta tagattggaa
    20341 atacaaagac caaccagagg taatcattga tagaatgaag aaaaaagaca ttaaagatag
    20401 tagtataatt ttaatgcatc caaccaatgc aacgacaaaa tgtatagatg atattatagc
    20461 tattgtaaga gaaaaaggac taaaaccagg aaaacttagt gatgtattta agtagacaaa
    20521 gtataaatat ttggttaaga ttgtataagt tgtagttgaa ttaattaaaa gactgactta
    20581 taatgtaata catataaaaa ataaatataa ttaaaacata gtgttttcaa taatccaata
    20641 tgaaacaaag aataaattgt taggagaatg taatgtataa gacaaaaata ctggaaaatg
    20701 ggcttacaat aattggtgaa gaaataccat atctaaagtc aataacatta ggtatttgga
    20761 taaatgcagg ctctaggata gaagaagcac aggtgagtgg aacttctcat tttatagagc
    20821 atatgatgtt taaaggaacc aaaaacagaa catcaaaaga gattgctagt tcaatagata
    20881 atttgggtgg tcaaataaat gcatttacaa gtaaagaatg tacatgttat tatgtaaaac
    20941 taatagatga gcatatagat acaggaattg atgtattgag tgatatgata ctaaattcta
    21001 aatttgataa aaatgatata gataaggaaa gacttataat attggaagaa cttaagatgt
    21061 atgaagactc tccagatgac ttatcttatg atttattagt ggaaaatata tatgctaatg
    21121 atggattagg aatgaatata attgggacaa aagaatctct gtacaatata actagagaat
    21181 ccatgttaga atacttaaat aagtattata ttccaaataa cgctgttatt tctatagctg
    21241 gaaactttaa ctttgatgat atggtggaga aaatcaaatc aaaatttgga cactgggaaa
    21301 agaaaaattt aagtatagac ataagtgagg ctaagtttaa tccttgcttt atatcaaaaa
    21361 ataaagatac tgaacaggtg aacttagcta tgtgcctaaa aggtattcct tttgaaaatg
    21421 atgaagaagt ctattcaatg gcagtggtaa ataatatttt cggtggaagt ataagctcaa
    21481 ggctttttca gaaaataagg gaagaaaaag gtttagtata ttcaatatac tcatcacaga
    21541 cattatatag gaaatgtggt gaattaggta tatttgcaag catgagtaca gaaaatttac
    21601 aagatgtata taatttaata aagaaagaaa ttgaaaatat aagagaaaac tatcttacag
    21661 aaaaagaaat atctgaaagc aaggaacagt taaaaggaaa ttatatattg gatttagaaa
    21721 gtactagcag tagaatgatg tctactggta aatctatgtt acttagtaaa aaagtaaaaa
    21781 ctacagatga aatacttgaa tgtataaata atgttaatat taattcaata aagaaggttg
    21841 ttgataaggt attcaatatc gaaaatattg gtacatgtat agttggaaga gatgtagaaa
    21901 agattctaca cttggattaa tattgaaaaa agtaaatctc tggaggtata aaatatgaat
    21961 ttatctgaaa tagcaggtaa ggaaatagtt aacttggtca caggagaaag attgggggtc
    22021 attggagaat gtgaccttat actagatgaa actacaggcg gtatattagc gcttctaatt
    22081 ccaaaagaaa aaggattttt tgccagaaga aaagataagt catttttaga agtaccatgg
    22141 agaaatgtaa aaaaaatagg taatgacatg ataattatag aatatgaggg atatatttag
    22201 gaggaaaaaa tgagtatttt agtgcaaaag tttggtggga catcagtaga atcatatgaa
    22261 aaaatgaatg aagtatgtaa gatagtaaaa gcatacaaaa aaaatgatga agaattacaa
    22321 ttagtattag tagtttcagc aatgggaaga aaaggagctc catatgctac agatacactt
    22381 ataaatttgt gtagtgcagt aaatgatgag ccaagtaaaa gagaacttga tttaataatg
    22441 tcttgtggag aaataatatc aggaacaatt cttgctaatt tattgaatgc acaggggata
    22501 gattctgttt tctttactgg tcagcaagca ggtataatta caagtgatga atattctaat
    22561 gcaaagataa agtatataaa tcctaaaaag gtaaaaagag ctttagatga tggtaaggtt
    22621 gtagttatag ctgggttcca gggtgtaaca gatgatggag aaataactac tcttggaaga
    22681 ggcggaagcg atacatcagc agttgctatt ggtaaagctt tagaatgtga aacagttgaa
    22741 atttacactg atgtggatgg tataatgact gcagacccta gagtagaacc aaacgctaaa
    22801 gtattgagtt ttatagatta tgaggaagta ttccaaatgg ctgacaaagg tgcaaaagta
    22861 atacatccaa gagcagtaca gttggctaaa agtggaaata taactttagc cataaagaat
    22921 acaatgaatc caacttttga aggtacaaaa ataggttctt tatgtagaca cttagaagac
    22981 aatatagaat atgaacaaga aagagatttt aaggttgctg ttgcaaataa agatagcgta
    23041 gcacaagtta agattaagtc agcagaagaa gtgtttacag aagtattaaa tgagatagaa
    23101 aagaaactta taactataga tatgataaat ttctttatat cagaaaaagc atttgttgta
    23161 gaagatgctg atataaaatc attaaaggaa atactagata aatttgaatt agattatgaa
    23221 gtaaatagag actgtgctaa ggtaacattg atatgttcaa gaattgctga tgaaatgtct
    23281 ggtatcatgt caaaggtagt tagaggatta tcaaaagcag gggtatctct tttacaaaca
    23341 tcagactcaa atatgactat atcttgttta gttagtgaag aagatatgca tacagcagta
    23401 catgctatac accaacaatt ttatttaaaa taaactgtat aatacaatta ttttttaatc
    23461 aaatttaaga atttcatata tatcttaata tatttgattt atttggtaaa actaaattaa
    23521 atataatttt gataggagtt tttttatgaa agattataga gaagataatg aaagatttga
    23581 taaagataat tgtagtgaag aatctagttg cgaagagaga gaatgtataa agcaatttgg
    23641 taccaatgaa atgccacctc aacctcctaa agatattcaa tgtataacta taattggaga
    23701 aattgaggga cattttatag gaaatcctca aaaaaaagct acaaagtacg agcatataat
    23761 tccaatgcta tactcaattg aggaaagtaa tgatgttaaa ggagtgttag ttgttttaaa
    23821 cactgttgga ggagatattg aagcaggttt agctattgca gaacttttaa acagtacatc
    23881 taaaaaagta gttactcttg ttctaggagg aagtcatagt ataggtgttc ctcttgctac
    23941 agcaggagat tactcattta tagcaccaac agcaaccatg attatacatc cagtgcgaac
    24001 tactggtctt gtgattggga taaatgagac ttttgagtat tttaaaaaga tgcaagatag
    24061 aataattcaa tttataataa gaacatcaaa tatcaaaaaa gatgtacttg aaaaacttat
    24121 gcatgaaaaa gatgaacttg taagtgatgt tggtagtgta ttaataggaa aagaagctgt
    24181 ggattatggt cttatagatg aagttggagg actaaaagaa gcacttaaaa aattaagaga
    24241 gcttataaaa gaatctgagg aagaaaaaaa taatgattaa aatttgttta aataataaaa
    24301 tttatgatat aatgatgtaa taaagtagga gtgttagtgt taaggcatta gcactttttg
    24361 ctgtgaaggg gtaaaacttg ctaaaaataa ggagtgatat taatggccac gaaaaagaag
    24421 aaaaaaaaga aggtaaatac cttaagtttt aatgcagaat atcataattt aataacaata
    24481 tttataggtg tatttttatt atatagttta aattctaatt ctatgggatg gatacctgtg
    24541 ttaatgcaaa atctttttaa gggactattt ggagggcttt caatagcaat accatttata
    24601 gtaattataa caggactttt aggatttttt gatggaaatg aatatatata taggctaaga
    24661 aagactaaac tatactacat aattatttta tttatatttg tattttatgg attattaaat
    24721 gcaggaactc ttcctgtaga tagcccacta aaaggtaaca tgtttgatga tgttatgaaa
    24781 ttgggtgtaa gtggacaagg tagtggtttg atagcaacaa ctattgcgta ttatatgagt
    24841 aaaatatttg gtatagcagg tggatggtta ataagtatct tcgcacttat actttctgta
    24901 atgtttatat ttaatatttc tataaaagat ttggtgtcaa atgcaaaatc aaaatctaaa
    24961 gcatctaaag acagcaattt aacgtttaaa gataaaattg caaatatgaa aaaatctgca
    25021 atagatatga tgactgatga agtagatgat actactataa ataaaaaacc aggtttcttt
    25081 aaagggttaa tgtcaaaagg tcttaataat gatgatgaag aagatgaata ttttgaagaa
    25141 gaaaatagtg atggtgtaga tgataaaact ataaaaatag ttggatttaa taaggcagaa
    25201 gatgagtatt tagaaatatt agaaggaact caaagtatgc cagagttaga tgttttaaaa
    25261 gaattacaaa aagctaccaa tgaaaatcca gttgtagata ctaaaccaga aaaaaaagtt
    25321 gatatagcta aacctaattt aaatatagaa aaaactcaac ctatgagtat tgtagcagag
    25381 ccagttaatg aggattattc aaactataaa aagcctagca ttgagctttt aaataaagta
    25441 aataagaagt ctgatgaaaa tggtaaaaag aaagtgttga aaaatgcttc tcttttagaa
    25501 aagacactat cagattttgg agtggaagca aaaattaatc aagttacagt tggacctact
    25561 ataacaagat atgaaataca accaagtcca ggagtcaagg taagtaaaat tgtaaattta
    25621 actgatgata tagctttaag tttagcagct aaaagtataa gaattgaagc cccaattcct
    25681 ggtaaaagtg caattggaat agaagttcca aatgaagaag ctcaaatggt tggtgtgaga
    25741 gaagttctag aaagtgaaga atttaataat tttaactctc ctcttgcaat gggacttgga
    25801 aaagacgtag caggaaaaat tataattgga gatataggta agatgccaca cttactaata
    25861 gctggttcta caggttcagg aaagagtgtg tgtgtaaata cattaataag tagtatttta
    25921 tataaggcaa atcctgatga agtaaaatta ctattaatag acccaaaagt agttgaactt
    25981 gcaaattata atggaatacc acatctattg ataccagtag ttactgaccc taaaaaagct
    26041 gcaaatgctt taaattgggc agttacagag atgaatagaa ggtacaaatt atttgcagat
    26101 gctcaagtaa aagatgtaac aagttataat gagaaagcgg aagaaaaact tcctaaaata
    26161 gtaataataa tagatgagtt agcagattta atgatggcaa gtgcaaatga tgtagaagat
    26221 tatatttgta gattagctca aatggcaaga gctgctggaa tgcacttgat tgtagcaact
    26281 caaagaccat ctgttgacgt tataacaggg gtaataaagg ctaatatacc atctagaata
    26341 gcatttgcag tatcatctca aactgattca agaacaatcc ttgatatggg tggtgcagag
    26401 aagcttctag gtaaaggtga tatgttattc tatccacttg gtgcagcaaa accagttaga
    26461 cttcaagggg cattcatatc agagagtgag tcggaaaaag taatcgattt tgtaaaaagt
    26521 caagttaaag atggaataaa atatgaagaa gatataattg aaactatatc taaggttaat
    26581 acttcaaagg gtagtgatga ggatgagttt ttgagtgaag cgatagaatt tgtcgtggaa
    26641 agtggtcaag catctgcctc tatgctacag agaagattta aaataggatt caatagagca
    26701 gctagactta tagattctat ggaagaaaga ggaataattg gagcaagtga gggaagtaaa
    26761 ccgagaaagg tattaataag caaacaggat ttgcagaatt tagaaggtga atagaattgt
    26821 cagttataaa aatagaaaaa gcacttaagc tagataaagt aatatttgta gatgtgagaa
    26881 ctgaaggaga atatgaagaa gaccatatat tgaatgcaat caatatgcct ctttttaaaa
    26941 ataatgaaca taatgacgtt ggaacaattt ataagatgca gggaaaacat gaagcaatac
    27001 aaaaagggtt tgactatgta tcgtataaat taaaggatat atatttacaa gcagcagaac
    27061 ttgcattaaa ttatgataat attgtcatat attgtgcaag aggcggaatg agaagtggca
    27121 gtatagtaaa tttattatca tcattaggtg taaatgtata tcaacttgaa ggtggatata
    27181 aggcttatag aaattttgta ttagagtatc taaggcatgt aatggataca aaaaactttg
    27241 tatctgttca tggattgact ggtacaggaa agactgattt gttacattta ttagatgaaa
    27301 aaggaataga tgtactggat ttagaaggca tagctaaaaa cagtggttct acctttggtt
    27361 ttataacttt tgataaaaag ccaccttctc aaaagaactt tgaaacaaaa ctatttgaaa
    27421 gtatattttt ttcgaaagag aattatatat ttgtcgagag tgaaagcaaa cgagttggaa
    27481 gtgttacagt tccaaatgaa attttcgagg caatgacacg agaaggacat catatattat
    27541 tagaatgtga tattgaaaat agagttgata gactttgtag agattatatt tatgataaag
    27601 atgaaggtaa tatattgatt ttaaaagaat gtataaacaa gtttagaaaa agattgggta
    27661 ataaagtagt agatgattat attgatttat tagaatcagg tgaatataaa gaacttgtaa
    27721 aaagatatct tttagaatac tacgacccac tttatatgca ttcagtagaa aaatataagt
    27781 atagtaagat tataaatttt aatgatacac aaaaagcttt agaagatgtt atagacttat
    27841 atgagagtat attggaagga gaaagtgaat gttaaaaata gcattagaat cattaggatg
    27901 ctcaaaaaat ttagtagatg cagaaataat gatgggaatt ttaaataaca aaggctataa
    27961 attaattgga gattttgaag aagctgatgt aattatagta aatacatgtg gatttataga
    28021 gtcagctaaa caagaatcta tagatacgat aataaacttt gcagagttaa aaaaaactgg
    28081 caatttaaag ttgttgatag taacagggtg tctagcccaa agatattctg aagaattaaa
    28141 aactgaaatt cctgaaatag atgctatagt aggaacagga agctatcaaa atatagataa
    28201 aatacttaaa gaattaagtg aaatgcataa aattgtaagc cttaatgata tagagtttgt
    28261 atttaatgaa gatttaccaa gatatatatc tacacctagc tatatggcct atttaaaaat
    28321 tggagaagga tgttccaata attgtactta ttgtataata ccaaaactaa gaggtaaata
    28381 tagaagtaga aaatttgaag atataattaa agaagctaag aaattagctg aatctggagt
    28441 aaaagaactt gtggttatag cacaggatac gacaaagtac ggttttgatt tatatggaaa
    28501 agaaagatta tcagaacttt tagaagaact tgcaaaaata gatggattta agtggataag
    28561 ggttatgtat tcatatccag aatctataac agaagaattg atacaagtta taaagaaata
    28621 tgataatata tgtagttact ttgatatgcc aatacagcat gcaagtaaca atattttaaa
    28681 attaatgaat agaaaaacta ctaaagaaga tatattaaat aaaatcaatc tgataagaag
    28741 taatatacca gatgctatac ttagaactac aataatagta gggtttcctg gtgaaacaga
    28801 agatgacttc aaacaacttg tggactttgt tgaagaggtt aaatttgata gacttggtgc
    28861 atttgcatat tcaagagaag aagatacacc agcagataga ttgccaaacc atatagatga
    28921 agaagtaaaa atacaaagaa gagatacttt aatgatgatt caacaaaaaa tatctgaaga
    28981 acttaatgac aaaaagatag gaaaaactta tgaggtgtta atagaagaac agatagaaga
    29041 taatgtatat acaggaagaa cacaaggtga tgcagaagaa attgacagta tagtgtatgt
    29101 aaaaagtgta gacaatcttg aagtaggaga atttgtaagt gttcaaataa atgatgcaat
    29161 ggaatacgat ttaatgggag atgttttata tgaacttgcc taacaagtta actttgttta
    29221 gaatattttt aataccagta tttgttttaa ttatgttgct caatatacca aataaatttt
    29281 taatagcttg tattattttt ataatagctt caattactga tgctttagat ggaaaaatag
    29341 ctagaaaata caatctagtc actgactttg gaaaatttat ggacccatta gcagataagt
    29401 tattggttat ttcagcactt acatgtatga tagaagacca tcttgtaagt agttggatgg
    29461 taattataat agtggctaga gagcttacag ttagcatatt aagagctata gctgctgctg
    29521 atggaaaagt aattgctgct ggaaacagtg gaaaacttaa aacgataact caaatggtat
    29581 ctatagtgtt tttattgtta ggtgctcagt ttgaaaatgt attgatatta aatattggtg
    29641 aaattctcat tcttatagct acactactta cattatattc tggatgggaa tatctatata
    29701 aaaataaaga gctttttatg tcttctaagt aaaaaattat gctttaataa ataaaaacta
    29761 atgattaata aataaaaaat aacaacttaa aattttaaaa atgtaaaaaa gggtatacta
    29821 ttatagtata cctcttttca ttttaagaat attttctttt gtgtatataa ttattaataa
    29881 tggtttataa ataaaatgta atgaataaaa atataatcat tagtagcgtt tttaaattga
    29941 ctaggtttga tttatgttat ataattaata tataacaaga gaacaaatgt ttgtagataa
    30001 atgaagggat ggtatagaga tgagtgtaga tcaagaaaaa ttaaaagcgt tgaatgaagc
    30061 tttaggtaaa attgaaaaag attttggtaa aggttcagta atgaaattgg gagaagcaac
    30121 atctatgtct atagatgtta tatcaacagg agcgattggt ttagacatag ctattggtat
    30181 aggaggtcta cctagaggga gaatagttga agtatatggt ccagaatctt ctggtaagac
    30241 tactgttgcg cttagttgtg tagcatcagc tcaaaaagat ggaggaatag ctgcatttat
    30301 agatgcagaa catgcacttg acccagtata tgcaaaagct ttgggtgtgg atgttgataa
    30361 cctaataata tctcaaccag atacaggtga acaggcttta gagatagcag aggcattgat
    30421 aagaagtgga gcgatagata taatagtaat agactcagta gcagcattag ttccaaaggc
    30481 tgaaatagat ggagatatgg gtgattctca tgtaggttta caagctagac ttatgtcaca
    30541 agcacttaga aagttaactg gttcaattaa aaaatcaaat tgtgttgcta tatttataaa
    30601 ccagttaaga gagaaagtag gaataatgtt tggaaaccca gaaactacta ctggaggacg
    30661 tgcactaaaa ttctattcat cagttagatt ggatgttaga aaaatagata caataaaaca
    30721 aggtgataaa gttataggta gtagaactag agttaaagtt gttaaaaaca aagtagcacc
    30781 accatttaag caagctgaat ttgatataat gtatggagaa gggatttcaa aaattggaga
    30841 ccttttagat atagctgctg atgtagatat agttaaaaaa tcaggttcat ggtatagtta
    30901 caatgatact aaacttggac aaggaagaga aaatgttaaa aaattcttgg aagataattt
    30961 agatttaact actgaaatag atgagaaagt tagagcattt tacaatttaa atgaagaaca
    31021 tgaagaatca ggtacttcag tatcaaaaga aattgtagaa gaataagtaa aaatacaaaa
    31081 aaataactag taattaaaga catgccacgc tccctattag cttgacacaa tgttgaaaaa
    31141 attataaaat taagtgagga taaaatatct aaatttaatt ttaattatta ggaatatttt
    31201 ttaatcatag tgatgacatt ttgggattat taacgaagga ggtggatgtc atagatagtg
    31261 tagtaatgat tgtaataggt gcagctgttg gtattatagc tggatatttt gttagaaaaa
    31321 atatatctga agcaaaaatt ggacaagctg aaaacttggc caaagaaata atagataaag
    31381 cacaccacga ctcagaaaca gtacaaaaag aaaaattact agaagctaaa gaagagatcc
    31441 ataagtggag aacagaagct gaaagagaaa acagagaaag aagaacagaa gtacaaaagt
    31501 atgaaaaaag agtagtacaa aaagaagaag tcttagatag aaaattacaa aatttagaat
    31561 ctaaagaagt aaacctaagt gaaaaattaa aggtagtgga aaagaaagaa gaagaagttg
    31621 aagtaataaa aactcaacag ttagaaaaat tagaaagtat atcaggaatt acttctgata
    31681 aggccaaaga aatcatctta accaatgcgg agagagatgt aagacgtgaa atgtctataa
    31741 tgataaaaga gatagaaagt caagctaaag aagaagctga taagaaatca agagaaatta
    31801 taggatatgc aattcaaaaa tgtgcagcag atcacgtagc cgaaactact gtaacagtag
    31861 taagcttgcc taatgatgaa atgaaaggta gaataatagg tagggaaggt agaaatataa
    31921 ggactttaga aaccttaaca ggtatagacc ttataataga tgacactcca gaagccgtaa
    31981 ttttatcagg atttgatcca ataagaagag aaattgcaag aattgctcta gaaaaattaa
    32041 tagcagatgg aagaatacat ccagctagaa ttgaagaaat ggttgaaaag gcgagaaaag
    32101 aagttgacaa tattataaaa gaatatggtg aacaagctgc atttgaaact agtgtacatg
    32161 ggattcatcc ggaattaata agattattag gtagacttaa ctatagaact agttatggac
    32221 aaaatgtact taaacattct atagaagttg ctcatatagc tggtataatg gctgctgaaa
    32281 taggagcaga tattagattg gctaagaggg caggtttatt acatgatata ggtaaggctg
    32341 tagaccatga aatggaaggt acacatgttg aaataggtat ggatttactt agacgttata
    32401 aagagtctaa agaagttata catgccatga gtactcatca tggagattac gagcctcaaa
    32461 cagtagaggc tgtattagtt acagcagcag atgcaatatc agctgctaga cctggtgcga
    32521 gaagagaaac tttagaagca tatataaaga gacttgaaaa attagaagaa atagctaatt
    32581 catatgaagg tgtagaaaaa tcatttgcta tacaagctgg tagagaaata agaataatgg
    32641 taaaaccaga agctataaat gatgaagaaa tacatctatt ggctagagat atgactaaga
    32701 aaatagagga tgaacttgaa tatcctggac aaataaaagt aagcataata agagaaacaa
    32761 gagctataga gtatgcgaag taaaagaaat aatatctaaa taataagaaa atatttttag
    32821 aaatttaaat ttgccacaag atatgataat atatcttgtg gcttttaaat tgtttaagtt
    32881 taattttata taatgcaaaa ataatctaag tgctaaaagt gctacttaca aaagattaat
    32941 aaaataaagc tttatatatg tattatttat ataattatcc aagtttgttt gatataatga
    33001 agttgtaaaa taaatatatt actacttgga atgcttgtac ttaaataaga tgaaaataca
    33061 atgacgctaa agtgaggatt agagagaggg tacactatga aaaaaaatac acttttacat
    33121 acaaatatta tggtttgcat tattattact cttggttttg tggctacctc tataattgga
    33181 tatcaatcta ataccaatat ttttaaaaat gatattgaac atgtatctac attggctgct
    33241 gaaggtattt attatcagat agataaacta ctttcagaac ctataaatgt atctcttaca
    33301 atggcaaatg acagtctatt aaaaaatttt ttagatggtg agaaagaaca tttaaatgat
    33361 gaagaattta tttataaatt acaagattat ctgaatgtgt atagaaataa atattcatat
    33421 gattctgttt ttttagtatc aactaaaacc aataattatt atcattttaa tggacttgat
    33481 agaaatctta gcgctaataa ctcagagaat caatggtatt atactttctt aaagaatgat
    33541 gatgaatact cattaaatgt tgataatgat gaagcaagta acaatagtat tacagtcttt
    33601 gtaaattgta agataaaaga tgataatgga gcaactatgg gaattatagg tgttgggtta
    33661 aaggttaatt cattacaaat gttgcttaaa ggatataatg acaaatttga tgttgttgca
    33721 cgcttaattg atgataaagg gtttgtacaa ttagcagtag ataaaaccgg tcatgagaat
    33781 ataaattttt ttgaaaattc tagtagcgac ttgtctaatt cgaaacaatt aatattaaac
    33841 aacaaaaagg aacaaaagag tttttggtat tcatctgaaa aatcaaaaag ttatattgta
    33901 tgtcagtaca tacctagttt aaaatggcat cttgttctag aaaatgattt tactttaatg
    33961 attaaacagc ttcatctgca attttttaaa ggcattgcag ttatagtttt aattataata
    34021 tttgtacttt ttactattac acatgtgctt aaaaaataca aaaaccaaat aataaagctt
    34081 agtgtttcac agaaattgga atatcaaaaa ttgttgagca aatctactga aggtctttat
    34141 gaaaatattt ttgaaattga tattactaac aataaagctg gttgtgaaga tgagaatagg
    34201 tattttcatg aatttggatt agggcttaat accccatatg atgaagcctt acatacaata
    34261 gctaaaaacc aaatcaaaga agaatatgca caaggttatt tagatacatt tttacctgaa
    34321 aatgtaatta aacgttataa tagcggaatt aataatatca gctatgattt tttaattaaa
    34381 aaagatgaag aaagttatca ttggattaga attaatgcac gaattttctt ttggacatca
    34441 gatgaatctg ttcgtatgat atcttatcgt aaaaatatag atgaagaaaa aaatagagaa
    34501 ttactacttc tcgaacgttc acaacgtgat tctcttacag ggctttacaa taaagctaca
    34561 acagaggaat ttattcgttc atttttaaaa aatgaaagta atcaagattt agaacacgtt
    34621 tttctaattt tcgatattga taattttaaa aatgtaaatg ataaatatgg tcatgtattt
    34681 ggagactttg ttataagtga atttgcctta gaattaaaat ctcaatttcg tgaggacgat
    34741 attgtgggac gtattggtgg agatgagttt gtagtattaa taaaagactt taataataat
    34801 aactatgtgc ttatagacaa attagagcgt ttttgttcta agattaatca gaaaaaattt
    34861 gttgagcaag cagaattttc tattgcatgt agtattggtg tagcaatgta tcctgaacat
    34921 ggatatgtat actctgaact atatgagaaa gcagaccaag cactttacta tgcaaaaagc
    34981 catgggaaaa attcttatca tatattttca gaggattcta ttgatagtat ttcatttcat
    35041 atagactcta gagatataaa agtattatta gaaatggcta ctgatggtgt tgtcaaattt
    35101 gcttgtatgg aaaacttaaa attactttat ttcaaccaaa aaatgcttga atttactgga
    35161 tattcagctc aaactgttac ttctataggt tttgatattt tttcgcctgt attacaagac
    35221 gacttacaaa tggtactaga tacattaaga gaagcaattt ctaaaagaga gacttttgaa
    35281 atagtttttc gtttttgtca tacagatggt catatttttc ctgtaaggat gcaaggtaag
    35341 tttgtaagtg aattatatca aaatcgttat cctgtttttt atgcgattta tacagacata
    35401 tcaagtatca atactttact agaaaataaa tagtaaatac tacagtaaaa tctaaaaata
    35461 ggatataata gtattaatat aagaagatta taaataaaac ctgctaatga aaatagatta
    35521 tttttagcag gtttttaaat tttttataat aaatcttatt tttggctaac aagatttatt
    35581 ttttttaaga ctttacttat tattttaagc attaattatg aaatttatca ttttattatt
    35641 tacagaaagt attttataac aataaatatc tatacttatt gcaaaatttt tttagcagtt
    35701 ttgtatttat ttgtatttat tttgaattta tttactaaat ttgcctataa aaagtatata
    35761 taaaaatata gttcaaaatg gtataattaa gtataacaga aggaggtaat gtaagtgagc
    35821 caatacataa atataaattc tcagttagac gccctaaaat tgaaggagtt cgagaaaata
    35881 ttttacgaaa atatcaatga ggacgttaaa gtagtgccaa aagttactcc ttttaaagga
    35941 ataaataccg atttgttgta tgttaaagat ggaagaattt tatttataaa atttatggat
    36001 acaacagaag atatattttt tattttagaa gaagagttac ttgaagttat gaatgaggaa
    36061 tatgaacttt tgaaactaaa aatgggacaa aagaatagaa atataagtta taactacgtt
    36121 tacattatgc catacgttga agttgaggaa acatatgaat ttgaagaatt tgttaataat
    36181 aatataatag ataaaaataa gcttcaagat ataatgaata agggttcgct ggaggaatat
    36241 cttaatgatg agaatgatga aataaactta aatttatttt tactagacat atgttcagaa
    36301 tactatataa taaatgataa acttcatcta aatgaaaaat ttaaaaaaat ttcattctac
    36361 aatgatgact ataagtatac agcaactatg atggaagacg ttcaaattaa agatgttgtt
    36421 tctattaaat atgggaatac acttattgaa ggtggttctg gtataggcaa aacagcaatt
    36481 atgctttcta gggctataaa attagcaaaa gtatatccac atcataaatt agtcatattt
    36541 acacatacaa aacaactgtg caatgagctt cgagagagga tagagttatt atacaaagat
    36601 aataacaatt tagaagttca cacatttagt tcttttatat ttaaactagc taaaaaattc
    36661 aatttaattg tagattataa catgttaaaa aatgattacg aaaagacctt taataatctt
    36721 gtaaaacaag cacagaacat tataaaaaat aaaaatatgt tcaaggctat ttttattgat
    36781 gaagcagaaa gctttttaga gtatgaaata gattttataa gggagttttt atataaaaca
    36841 aaattcatat ttaatgtgtg ttcttgtaac tccttgaata tctctaatag attgaatata
    36901 tttaaaaaat tatataatgg tatagaattt gatgataaga ttatcttaag taaaaattat
    36961 agacaagcta aagagattgt agattttaca aataaatttt caaataattc taactcatat
    37021 atcaacgaat taagaccaaa tacagaattt agtactttct tttatactaa agcacttaga
    37081 ggtggaaata agtctgttga tataataaaa gtaagtgacc ttgatgacca aataagttct
    37141 gtaatctggg aaattgagta tttaataagt aaaaaaggtc ttgattattc tgaagttgct
    37201 atcgtttatc cttacaataa gaagaaatta aaaagtggaa aaaccattta ctttcaatat
    37261 atgttaaaaa aagcattgga agaggcaaaa attccttata tatgtgcaga agataatttg
    37321 acaaatataa gtaagaaggt aggaactaca atagcaaata tatatgcaat aaagaattta
    37381 gagtataaag cggtaatagt atgtgaatta gagatgttat acaatcaaac tataaatgat
    37441 attgaacaag attatcaagt aaatgacttt gtaggagatt taaataaggt ttatttagct
    37501 atgagtagag caacagacta cctaagtata gtaacaactt ttaatgaaga agcaagtgat
    37561 ataataagac ttattacaga atcaaaagat atttagtaaa aaaatatttg taatttaaga
    37621 aaataaaaag agactgagat ttgtaaaata aactttacca attcagtctc ttatttttta
    37681 gtctactttc ccaatattat caagaggtaa tagtctcacc ataaccttac caagaacttc
    37741 atcttcatca accataccta catcagggaa tctactatca ttactatttt ctctattatc
    37801 tcccattgca aatagtttac cttttggaac aacagtatct atatctccag aagtatagtt
    37861 attgtgtata tatggttcat ttaataattt tccatttaca tacactttag aatttgatat
    37921 ttttattctg tcaccttcag tagctataac tctttttatt aagtcttttt tctttcctgt
    37981 ttctccatca actaaattgg ttttaaaaac tacaatatcg cctttttcag gtttaccaac
    38041 cttgtatgat atcctattta aaatcagata atctttatca tctaaagtag gatacattga
    38101 ttctccactt acaatagaag gtcttataaa ttgtagaatt acaaatgcaa gtacaatagc
    38161 catagctatt gacttaatcc aatcaaatat ttctttttta acactcataa cttttctcct
    38221 tatcatttgt tattaaatta gaatacatta taatagaaag aaataagcaa gaaccactat
    38281 acccaagact attctataat aaccaaatac tttgaagtca tgttttttga tatagtccat
    38341 aaaaaattta ataactacaa ttgaaacaac aaatgctact acagaaccaa cacctaaaat
    38401 aagccattca aatccagtaa atccaaatcc agcttttaga agttttaatg cactagctcc
    38461 aagcattgta gggatggcta agaagaatga aaactctgct gctacatatc tagaagtacc
    38521 aagtaatact gcacctatta tagtagaacc agacctagat gtacctggta taagagctag
    38581 acactggaat aatcctatac aaagagctaa tttatatgta acttgagaaa aatctttata
    38641 cttaggtttt ttatttctct tttcaagccc tatcattatt ataccatata caataagagc
    38701 aatagcaact actgttgaat taaagaataa tctatctata tcatcttcaa acagtattcc
    38761 aataactcca gatggtataa ctgcaattat aaccttaagc cataaacgga ctgtttcttt
    38821 tttctgctta atattctttg cgctatcaaa aggatttaat tttctaaaaa atataacaag
    38881 tacagctaat attgaaccaa attgaattac aactaaaaat gtacttataa atgtatttga
    38941 aaaattcaat tttataaact catcaactaa tatcatatgc cctgtactac ttactggtag
    39001 ccattctgtt attccctgaa ctatgcccaa aatgactgcc ttaaaaattt caagcaaact
    39061 aaatgtttcc aaatcaaaat acctccaata caaatttata gtagcatatt ttattcatag
    39121 attaataaaa aaatgccacc ttttactaac ttataatacc atagtactgt gtagtgcaca
    39181 atggaagatt gatattttca taaaaccttc ataatataac aaaataaact taaaagttat
    39241 ctattgaata aggggtatat ttgaggggga aatatatggt atagtaaaag tatagctatt
    39301 ttattttatg aaaaggaaag gagttattat gtatagtaca aaaatactag atgattatga
    39361 gtatgaaact tttaatgttt tttctacaaa attaaatcca aacacgaaac acgattattt
    39421 atcaaaggtt atactgttta aagaattttt aaaagggaaa gaattgattt atgctacgaa
    39481 agaagattgt aaaaattttg tagactatat acagactaaa tatgcgaaat caacttgtga
    39541 aaagatatat agttatttac atagttttta taacttctta aaaaaagaag gatacataga
    39601 tataaatcca tttagatatg tagaaaaacc cacagtaaca agaataaaaa caaaagatga
    39661 tgttctaagt atacaagaaa taaataaact tataggtatc ctaccaaaat taaatataag
    39721 agatagagta ataatagtct gtctagttac tactgggtgt ttattaaatg aattagttag
    39781 cttaaagtgg aaagacctta tggtagatga aaacgataac tcatatgtaa ggttaggtaa
    39841 gggaagaaaa gaaagagtag ttaaattaca tccatacttt tttaaacttt tagaagatta
    39901 caggaattat tctgggcttc cagaggtaat aatacctagt gatgatttta tttttacaac
    39961 tcaaaagagt aattctataa cagatagaaa tgtcagactt atagtcaaaa aagcattaga
    40021 tttagctgga ctttctcaat attcagcaag agattttaga cattcatttg cagcaataag
    40081 tttaaggctt ggggcagatg aaagtgatgt aaaaaatcag cttggatgga gtgataaata
    40141 ctatgcaatt agatataagt atgtattaaa ttttgttgat agtgaaatag tggattactt
    40201 aattgaaaaa gataatttgg atataaataa aaaatagtaa taaataaaaa atactatata
    40261 taaatgtgaa caaaaaataa aaaagtcttg ttatagttcg taaaattata tataattatt
    40321 tatgtactaa tgtgtacaca gtaatcacac gacaaaagat ttaaggaagg tgctaatata
    40381 atgatagata ataaatatac tacttactca aacccactta cagacagata ttgtagcaag
    40441 gacatgagtt atatattttc tccacaattt aaattctcta catggagaag gttatgggtt
    40501 gcacttgcag aaggtgaaca tgagttagga atcaatataa ctgaagaaca attagatgag
    40561 ctaagagcta atatagataa tataaatttt gatgaagcta gaaaaataga aaaagaagta
    40621 agacatgatg taatgtctca cgtaaaagct tatggtttac aatgtgaaaa agccaaagga
    40681 ataatacatt taggagcaac atctgcatat gttggagata atacagatgt tatacaaatg
    40741 aatgaagctt taaaacttct aagagtaaag cttgtaaatt taataaacaa tttaaaagaa
    40801 tttgcactta agtacaaaga tatgccagct cttggattta cacacttcca agctgctcaa
    40861 cttacaacag taggaaaaag agcttgttta tgggctcaag atttaattct tgatttggaa
    40921 gatttaaact atagaataga caacatgaag ttaagaggag ttaaaggtac tactggtact
    40981 caagcaagtt ttttaagctt atttgaagga gaccacgaaa aagtaaaaga gttagataag
    41041 aaaatttgta aaaagatggg ataccattct tcctatgcag taagtggaca gacatatact
    41101 agaaagttag attatcaagt aataagcata ctatcaggga tagctcaaag tatgcataag
    41161 atgactaatg atataagact tttacaaagt ttaaaagaat tagaagagcc atttgaaaaa
    41221 aatcaaatag gttcatcagc aatggcttat aagaggaatc caatgagaag tgagagaata
    41281 gcatctttat ctaagtatat aatctctgaa tctataagcc ctgcaatggt acaagccact
    41341 caatggttag agagaacatt agatgactca gccaataaga gattggcaat acctcaagca
    41401 tttatggcag cagatgctat acttgaaata gggataaatg taactgatgg tttagtagta
    41461 tatgaaaata tgataaatag acgtgtaaat gaagaacttc cttttatggc tacagaaaca
    41521 atacttatgg aagctgtaaa aagaggtgga gatagacagg aattgcatga aataataaga
    41581 gaatattcta tgaaagctgc ctatagagta aagcatgaag gcaaggataa taacttaatt
    41641 gaacttataa tgaatgatga ttcatttaaa atgagcaagg aagaaatact atctataatg
    41701 gacccaaaaa actttatagg tagagctcca gaacaagttg tagaatttgt aaatgaagtt
    41761 gtagaacctg ctataaaaga ttataaggaa gacttaggaa aagtagatgt agacctaaga
    41821 gtatagaatt gtacagataa aatataatta aaaacggcta tcttatttat aaataagata
    41881 gccattttaa tgggctagcg tcttctctcc accgctgtga agagttcttc tttacttact
    41941 ataccatgaa tttaataaaa tagctatata aataatattt aataggaata atataaataa
    42001 aaataaaaaa atgataagat tactaattaa atttaaacat tcagcaaaaa ctattgtact
    42061 tgcaatattt ttaatgtaaa ctgaaataaa gttatttaga taatttaata aattttaaaa
    42121 caaccgaggt gattatatga actatatagt aaaagatacc ataagttttt tagtaaaaac
    42181 atttcctaag atttattcta gcctgtactt agaagaccta aaaaaatttg caccagatta
    42241 taatgtaaat aaaacacaat taagagcatt agttttcata aaaaattatg gagtaataag
    42301 tatgactgat ttatgctcta agttaaatat agaaaaaggc agtttaacta gtatggttga
    42361 tgatttaact gataagaagt atgtaacaag gaaaagagat ttagttgata gaagaaaata
    42421 tctaattgac attactgaag agggagataa gatagcaact gattttatgg ataaattaag
    42481 tgatggttta ggagaaaaac tttctaaatt aactgaagaa gacagaaaaa aatatttaga
    42541 agcaataaat actcttcaat atatattaaa caaagaagaa tttagataga aaaaatataa
    42601 aaatttttgt atttaatggt actaagatta attctttgtt attcatttaa ctatataatt
    42661 ataagtctaa atacaatcta aaaagtacat cttaaatttt cttgatgtac ttttttataa
    42721 tttctctaac taaggcaaga caagttttag atgctttaat taaagtgaat aaagattact
    42781 ggccaacact atataaagaa aatgaaaaat agtcaggtct aagtcttaat taatatatta
    42841 attaagaaat gtcccctttt caaataatat ctgaaaaggg gacattttat atcattaaat
    42901 ttatataaat taagtttaaa aaattatttt tgtaaagcta catattgctc taaaccttct
    42961 tttaataatg tagcagctct atgaaggtct ttttcattta agatgtaagc taatcttatt
    43021 tcatcacgtc ctaaacctgg agtagcatag aatccttcag cagggcaaac cataactgtt
    43081 tcattatctt tattaaattc ttttaacatc caaattgtga aatcttctgc attttcaact
    43141 ggtaatttag caactatata gaaagcacca gttggttttt tacatataac accttcaacc
    43201 ttcattaatt cattgtataa tacatctctt ctttttttgt actcttcatt aacttctttg
    43261 aagtaagaaa caggagtttt gtataactca acagctccta tttgctctaa tgtaggaaca
    43321 caaagtcttc cttgacataa tttaagtatt tgagcgataa gacctttatt tttgcaagcg
    43381 atagaaccaa ttctagcacc acaagcactg tatctcttag aaacactgtc tattattata
    43441 actctatcct gtacaccctc taagttacca cagcttgtat actcaagccc atcataaaca
    43501 aattctctat aaacttcgtc tgctattatc cataaatctt tttctttagc tatttcagct
    43561 aggatattaa gttcttcttt tgtataaatt gcaccagttg gatttcctgg gttagaaaga
    43621 attatagcct tagttttatc atctacttta gacaatattt cttctttaga tggaaggtgg
    43681 aaaccgtttt ctgcctttgt tgtaacagca ttaacttcta cgtttacaga ttgaccaaat
    43741 ccattgtagt ttgtatagaa tggttctgga actaatagat tatctcctgg gtcacaaact
    43801 gccatcatag tgaaaagtaa agcttcacta ccaccgttag ttactaataa ttcatctttt
    43861 tcaaaattca tatcgtaagt tttatagtaa ttttgaagtg cttctaataa ttctggaata
    43921 ccttctgatg tagcatattc taagacttcg ctatcaaagt ttttaacagc atcaaaaaat
    43981 cctttaggag tttttatgtc tggttgtcct atgtttaagt gatatacttt gattccttta
    44041 tcttttgctg cttgtgcaaa tggaacaagt tttctgattg gagaggcttg cattgcacta
    44101 actctgtttg aataattcat tttaataccc cctatttaat ttaaatacgt atattgtatt
    44161 atgtaaattg tatgtttgaa tacaagacat ctttacaaga taataataac atacttatgg
    44221 gtaaaaatca tcaaaaaaaa ttaatatagc tttaaaataa acttttaaca aattatagta
    44281 aaatacccat gtttatgcca ctaaattatg tattcataaa ttaatttata gtaagataat
    44341 attctgatgt actgatagtg attttttagt tataatcttt cagctataga caaaatatat
    44401 aataataaat atgcaaaatt ataaaaaata ttataaattt atttaacaga tacgataaat
    44461 atcatataat tatattaaag gctattaact aagaggtgtt aattatgaaa gaattaagaa
    44521 tagatccaat taccaatgat gttgttatat ttgctacaga taggttaaaa agacctttag
    44581 atacagcaga tatcccaaat gaagatgaag aaactaatga atatgatgaa gaatgcccat
    44641 tttgcaaagg aaatgaagct catgcaactg atgcattatt tgaaatagat agtgaagaag
    44701 gatggcttgt aaaatctgtc tacaataaat ttcctataat tgatgatatg gcaagagatg
    44761 tatatggtgt tcatgaagta atgatagaga gcgataaaca taatcgtagt ttttataaca
    44821 tgagtcaaaa agaatttgaa gatgtttttt ttatgtatag aaatagattt agagatttgt
    44881 cgaaagatga taaagtagag tatgttagta tcttcaaaaa cttcttgaga aaggcaggag
    44941 catctttgat gcatccacat gctcaaatat tgtctatgtc ttttattcca cctgaaatta
    45001 cgaatgaact gttggtttca aaggagtatt atgacagcaa taaaagctct ttgtatgatg
    45061 atttaataga aaatgaaata agtttagata gaagagttgt ttataatgga gaagcttttt
    45121 tggtacttat accatatgca accaagtata gtggagaagt aagaataata tttaaagata
    45181 aaatagagtt tggtgagtta aatgataata atatacagga gctttctaca atatttgaaa
    45241 agttgtttaa gaagttatac aatatcaatg gatatatgcc atttaattta tgtattcaca
    45301 ctcatccaac aaagattgaa acaaaatcgt attttaatgt tcatatgcac ataatcccaa
    45361 gaaaatataa ttttggagga tttgaacttg gaacaaatat gtatgtttct tcaatgagcc
    45421 ctgaagattt aacaaaaaag ttaaaaattg attaaaacat atatttttag gtattaagta
    45481 aatagtaatt ttagatgaaa ttatagtata gcattgttaa aataaaagta aattaagaat
    45541 ttacaataaa attgttgaaa agcccccatc tttagctatg gggagtgtgt caatgtttag
    45601 atgaggagta taagtatgaa atttatatca tggaatgtta atggaataag agcttgtgtt
    45661 gggaaaggat ttttagactt tttcaaagaa gtagatgcag atatattttg tttacaagaa
    45721 actaaattgc aagaagggca aatagagtta gatttaccag gatattttca atattggaat
    45781 tatgctgaga gaaagggtta ttcaggtact gctatattta caaaaaaaga gcctttaaaa
    45841 gtaatgtatg gaataaatat agaagaacat gacaaagagg gtagagtgat aactctagag
    45901 tttgaagatt tttactttgt tacagtatat acgccaaatt ctcaatctga attaaagaga
    45961 ctagaatata gaactagatg ggaagacgac tttatagatt atttaaccaa actagataat
    46021 cataagcctg taattgtgtg tggagatatg aacgttgcac ataaagaaat agatttaaaa
    46081 aatcctaaaa ataatatgaa aaatgcaggt tttactaaag aagaaagaga gaagttttct
    46141 aaattattag acagtgggtt tatagacaca tatagatatt ttaatcctga taaagaaggt
    46201 gtttatagtt ggtggtcgta tagatttaat gcaagaaaaa ataatgcagg gtggagaata
    46261 gattattttt gtgcatccaa aaaattagaa gatagattag taagtgcgga tatacacact
    46321 gaaatattag gttcagacca ttgtccagtt gaattagaga taaaataaaa attaattatg
    46381 ataaaaattg ggctgtagat ataaatttaa agttaactag ttttttaatc tacatgccta
    46441 ttttttatct aaataaactc tatttgtttt ttctttgctt aacattttct aatgtaaatt
    46501 gatttgtttt ccacatatta taagctaagt taatgatttt tttctcgtct ttagttaatt
    46561 cacgatttaa aattttccta aattcatcaa ataacttatc ataatcaact ctttttttgt
    46621 tagaatatct tatagtttta ggttttatac tcgtataaag atatatatct gggactatac
    46681 ttggattgta tatcttaata aaaactttag cagtataata tgcatcattt aaagcattgt
    46741 gataagacat tttttcatca agttccaata tagaaatagc attttgtagg ccaatgcttt
    46801 ttcctgctgg attattgaaa tatttagatg catgttgttg tatattaata taagatttag
    46861 gtaaggaatt tgatggtagc ttgtgatagt tgatattcct gtataattcc ttcaaatctc
    46921 cagctcccca aacacataat atagggtctt gtgatgatat aaattttata aaattgttgt
    46981 agacctcagg gaaagtaggc gaatcattaa aatcagaact tttaatatta gtcatacgac
    47041 taataaaggg atgtatgtct ttataaattg taggttttac atagctatta aacgtatcta
    47101 ttatattgaa tttagaatcc aatttaattg ctcctatttg tataatttca aaaggacact
    47161 tttcattgga tacagtttta ttgagttttt tatcaaatcc ttggttaaat tccaaatcga
    47221 aaattatgta ttccataaag tttatctcct aaattttttt attatttctt ttactataga
    47281 ttatattata aataaaatta aattttcaaa taaaaatact tttattttag tataccccat
    47341 atttgtaaaa atgttaaact attgtatgta taatattttt tccaataaaa aatattgcga
    47401 actttttata cttgatatat aataatataa ataaaaacac gaataaaaca cgaataattt
    47461 gcaagaaaaa ttcataaatg taaggaaaat gaaatttatc ttgtatttaa caaaataaaa
    47521 tatacataaa aattatggga gagtggaaaa atggatagtc tattatacgt aatagagaaa
    47581 gataaacaca ctaatgaaga attaagagaa atcctaaaaa gcaataaaaa tataagattt
    47641 gtttccttaa tgggggttga tttaggagga aatgcaacag atgaaaagat tcctgtagaa
    47701 ttatttttgg aggatataga taaatttttg gaatcagcta tacagactga tggttcaagt
    47761 gttgagcttt ataatatagc tacattaaat aatgcgaaag tagatttaat gccagataaa
    47821 agttgtcatt ggtatgtaga ttacaatatg gaatatatag acgaagaagt aggtctacct
    47881 gttggaacat taaagatacc tgcattttta atacatgata ataaaaaagt atgttctaga
    47941 ggagtacttc aaaaagcaga taagtacttt aaaaagtcaa tgtatgaaat atttagagaa
    48001 tatccacatg taataaataa cataggaata gattcagtag atgatataga ggaaataatg
    48061 ttaacagcag ctacagaact tgagttctgg gttaatactc ctgaggataa agctgactta
    48121 gagaaattat atgtatcaca aagcctaaaa gagcaatact ggaaaagaac tcatggtatt
    48181 ataagaactt gtttagagaa aagtttaatt atacttcaaa agctaggtgt aaaccctgaa
    48241 atggctcaca aagaggtcgg aggaatacaa agttctatta gtatagatgg aagaacaaac
    48301 catgctatgg agcaattaga ggtttcttgg aagttttcta caccgttaca agcagcagac
    48361 aatgaattat tagtaagaga tgttatagag gatgtattta catcacatgg actagaagtt
    48421 acatttaaag caaaaccaat tcatggagta gcaggaagtg gtggacatac acatgtagga
    48481 gtaagtgcga agttaaagga tggaagtata aaaaatctat ttgcgccaaa agatttaaaa
    48541 gaagattatc taagtgaact aggatatggg gctcttatgg gacttcttta caattatgaa
    48601 gtattaaacc caattgtaac agcttctaat gatggattta atagattagt tcctggattt
    48661 gaagctcctg tatgcatagt tacatcttta ggtcatagtt atgagattcc atcaagaaat
    48721 aggtcagtgc ttgttggatt aataagagat atgaaaaatc caaagactgt aagatttgag
    48781 ttaaggtcac caagccctct atctaacacg tatttagtaa tagcaggatg ttatcaaaca
    48841 atgttggatg gtataaaagc agcagctaag agtggtctta gtacaaaaga acttgaaaaa
    48901 gagttatcta aaaatgtagg tgaagaaagt ttctatttag aaaaagatag agcatataga
    48961 gatgaaaatg atgtatttga acattatagt ttggaagaaa gaaatgcaag atttggaata
    49021 ccaccggcta cagtttatga aaatatgaaa aacttagaaa tatatgcttc aaaattaaaa
    49081 tctttaaaac aaggtgatgt atttactgat agtataatag aatcatttaa gattggagct
    49141 atagataagt ggcaaaagaa attaaaaact agaataatag aggctgggat tcaaaaaata
    49201 agaagtattg tcaagataca cacaaaagaa aatatggatg cgcttgatga ggtagtttgg
    49261 aactcaatat cggatttaaa gttcaatatt atgaaggata ctttaactag agaatcacta
    49321 ttcactagag tgagagaagc tatagaaaat aaggattacc agactgcttc agatttacaa
    49381 atagaattaa agagaagtat ggaagaaata caacaactgt atatgcagta caagaaaaat
    49441 atatactaat ataacaattt gattttgata tgataaaggc aatcaagtat tattaaacac
    49501 tatgagtaaa ttctcaagtg taaaagcata cttgattgcc tttaatttta tgtttgaaat
    49561 tttttaatag tacaatttaa ctgtaatgat tatctattaa ttctgatata taatcagcaa
    49621 ttttctctgg atgaatgact gttttaaatt cttttaaatt ttttttcata aagtttatta
    49681 tagaatcaaa ttcatttgat actaatttat aaaatatgtt tttaaggtct aggtcatcac
    49741 tataaatcat gccaagaccc ttatctatta taaatttagc gttttcaatt tcttgtccaa
    49801 cttttggcat ttttacgagt actggagttt gtgattgaat agcttcaaat atagttgctc
    49861 ctccaggctt agttagcatt aggtcacatt ctcttattag attatacatg tcgtttacaa
    49921 atccaagtac tttaatattt tttagaggtt ttttatgagt tagattgtca tataattttt
    49981 tgtttttacc agtaactatt gtaatttcta tagaattact atgttcttca ataaattcat
    50041 ctatccaatg cataaaatcc tcatctatat caaatagacc tcttcctcca cctagaagta
    50101 atagtctata tttatcacaa catagttctt tactttcaat ttgaaatctc ttatcaacag
    50161 gaactccagt aacctttatg gagtctggat ttattccttt ctgaaaaaat ctatttttta
    50221 tttcaggaga aggaacaaaa tacaaatctg tattttcaaa aacccattcc atactatcta
    50281 caacatctgt tataactgta agtgtaggta tatttatatc aggatttttt atcttaaagt
    50341 tatttacaca agcagcagct agtggaaatg tagagataat caaatctgga tttatatcag
    50401 caatatactc agaaagttta ggtgtatata atttatgaga taaatcatgt cttgaatcaa
    50461 acgatttctt aaagtaataa tagtaattat ataatccagg agtatatttt gtattgtttt
    50521 cataaagttt taccataggc ttattcatca tgggaatact tgcatttata aagttttgta
    50581 taacaacatt atagttacta tatttttcaa taatacattc tttgactgct tttgcagcac
    50641 ttatatgtcc agcaccaaat tgagcagtta ggattaatat tgttttatca tctttcattt
    50701 tagccaattt atcaccactt tcataaaata tatctgatta gagtattata catttataga
    50761 aggatttaat caacaagaat atttaaaaat aactattgtg cagaacatag tactgtgtta
    50821 ttatgaggat ataagaagtt ttaacatagt accttgtaaa acacaatact gtgtatttaa
    50881 agaaaggatg gagtatatga acacacaatt tagaaagggt gttttagaaa tttgtgtact
    50941 tgctctcata tctaagaaag atatgtatgg atatgagata gtacataaca tctctaaagt
    51001 aatagatgta aatgaaggaa caatatatcc tattctaaga agacttacaa aagatttgta
    51061 tttcgaaaca tatatactag aatctaatga aggtcctgct agaaaatact atagaataac
    51121 ttcactagga aaagagaatt tatccaatct gatggaagag tggagagaat ttgtaaaggc
    51181 tgttgagatt ttaataagtg aagatgaggg aggtaatgaa cttgaataaa actgagtttc
    51241 ttgaaatttt aaaggattat ctaaaaaaag atttttctga gagcgaagtt aatgatatat
    51301 tgagagatta tgaagagtat ttcgtagatg gtcttataga gggaaagagt gatatggaga
    51361 taatagcatc acttggctca ccaaaatcta tagcaaaaga acttatctta caaactaaag
    51421 aaaaaaatat tgaagaggaa aaaataataa atacagatgt tttgaatgat aagtttagta
    51481 aatttaaatc agatatgaaa aaaggttact ctatcttaaa atatgaagca aaagaaaagt
    51541 atagtaaatc aaaagaaaat atagatggaa aaataatgaa tacagacata ttaaatggta
    51601 aatttagtag atttaaatca aatgtaaaaa aggattattc tatcttgaaa gatgaagcta
    51661 aggaaaagta caataaatca aaagaaaata taaatgataa acttacacca agtatagaag
    51721 aaggtgtagg aggattatcc agacaagtta taaaggggtt attagtagtg ttatctttat
    51781 ttctaattgt tccagcattt gcgtttgtat gtgctattgt atctgctact attgcattag
    51841 tatgctcatt aattgctttt gttatattaa ttccatttgt gattaaattt atagcaatgg
    51901 ttccagatgt tatgatgttt tgtattttcg caaccatagc atttgtaggg cttgagatac
    51961 ttgcatggca aattttatta tttgccataa aactaggtaa acaattacta aataattata
    52021 tacattggat aaagagaaaa aatatttata taaaggcaaa tgaaaagatg gaaaaaagaa
    52081 aaaaagctac tttaaatgat gaagatttga ataaagacat taatgaaaat tcatataata
    52141 atgattacta caatagagaa gaagaaagca taaaagaaac tgatgaatat aatgatgata
    52201 gtataaattt tgaggcgata tataaggacc ttgaagatat agaaaataag aaaggagaag
    52261 ataaacatga ataaaaaact tgctatattt gctgcagttt tattggtaat aggtataata
    52321 gggacaacat ggtcagggat tttagttatg ccaagcctaa taaactttgg tttagaaaag
    52381 gaagctgaat taaacaaaga aaacaaactt tatcaagaaa aagtaaatat tgacaagtta
    52441 gatatagctg ttgacaatat aaatgttatg ataagaaaaa gttcaaatga agatgtgcgt
    52501 gtgacaacaa gagggaataa tgaattttac aaatacagtg taaccctaca agataagact
    52561 ttagttgtaa aaggcgaaag aaaatctgaa aataaaatta aaaagattag aagtttcgac
    52621 cacttattaa atacatctat aaatagtatg ttttcacgtg attatagaga aattagaata
    52681 tatgtaccaa ataatgtaga tataaatgca tcaagtataa gttcgtactt atttatatct
    52741 gatgatgtag attcaaataa tataacatat aaaacttctt ctggaggttt tagcagagct
    52801 ataacagaaa ataaagtaaa tagattagaa aacttaaact taatatctaa tagcaatctt
    52861 catctatcaa caaagtcaat tttaggcgtt aaaaatgtaa acatagaatc agagtcattg
    52921 gatatatctt cagagaatgg agatgtattt atcaataata tagaagagta tatacctgaa
    52981 aatgtaaata taaaagagaa aataggtaga aattcaaatt atgaaagtga attttacttg
    53041 tactctgata tgccaattgc taagttcctg gacatagaag ttccaaattc taaggtaaga
    53101 cttgatatac cagtaaataa gtataaattt aattgtgata taaagtcaaa agaagcaatt
    53161 gaagaattta ataatgatga agaatatgac aatgattcat atgattatga acataatgaa
    53221 gaacatagca acaagtatag aaatacgagg gaaataaaag ggcttttgaa taaaaatctg
    53281 tcgaatttag agaaagaata tactataaaa ataaattcaa atagcttaga attgtaaata
    53341 atttactata ataatttata aattgattta aaagtataaa aatataatct atactatggt
    53401 ataataagta tcgaaagaga attaggagag ggatacttat gaataaaata gcagtaagtt
    53461 ttttaataat tgctactact ttattgagta cggcatgtat ggattatagc atcagtgcag
    53521 ttgagctagt tgattctaaa gaatcagcag ttgtaaagaa ggatgaagat gcaaaagaag
    53581 aaacaactag taaaatgatt aattctaaaa aaactactaa aatacctata gagataattt
    53641 caaaggatga gaaaatagta aagtacttac agattgatga agaaagttca cttaaggata
    53701 aactgcgatt aatcttagat acattatcaa atgagtattt taatggttta gacatggaag
    53761 tagaagttaa ggaaaaagat aatcttgtta aaataaactt gatagaacca gataaaaaaa
    53821 gtagagtttc ttggaaagat gattatttga atgaacaaaa tataatatat actataaata
    53881 atattattaa aaatgtcatc caagaagaag ataatagcat ttggattgaa gaagtagaga
    53941 tatactacaa tggaaaatta atagaattaa gataaaaaaa gatgtgtttt taacacatct
    54001 ttttttgttt tttaattctt ataaaatcct tatatttatg atttagaatg attcttgtaa
    54061 ataagtttga caaaagaaag gatgaaaatt atgagtaatt atattttgca ggtaaaagga
    54121 gtttctaaaa aatttaagga gcagatggca gttgataaca tttcactagc aattaaatct
    54181 aattctatat atggattatt aggaccaaat ggagctggga aatctacttt attaaaaatg
    54241 ataactggga ttttaaatcc aacttcagga aaaataatgt ttgagggaca tagatggact
    54301 agaaaggatt tatcaaatat tggttctctt attgagtctc ctgctatata tgagaattta
    54361 acagcacgag aaaatttaaa agtccatact actttacttg gtcttcctga ctctcgcatt
    54421 gaagaagttt tagagactgt agatttaaga gataccttta aaaagcgttc aggtcaattc
    54481 tctctaggca tgaagcaacg ccttggtatt gctattgcat tattaaatca cccaaaactt
    54541 ttaatcttag atgagcctac aaatggtctt gaccctattg gtattgaaga acttagagaa
    54601 ttgattcgtt cttttccaaa acaaggaatt acagttattt tatcaagcca tatcttgtct
    54661 gaagttgaac aagttgtaga tgaaatagga ataatttcta atggagtaat gggttatcaa
    54721 ggagaagtat caaaagaaca aaacttagaa gaacttttta tgaaagttgt agctgaaaat
    54781 agaaagagag ggcagtaata tggaaagatt tattagagtg gaatttcaaa agatgaaagg
    54841 aacttttgga atgaagttga tatggatagt tccagtgttt tgtatacttt tgtgtggact
    54901 aggtggaggg caaaatgctg ctttcaactg gtggtatacg acatttcttc caggagcatt
    54961 aacattaata tcttctttaa tagtattaaa agatgaaaaa agtaaatatc gtggtatttt
    55021 accattgcca attgaaatga aagacgtttg gactggaaaa atgatttact gtattatttg
    55081 ttgttttata gcatcaatta tttctgcaat aggtgtagtt tttgttggga taatctataa
    55141 agaaactatt tcaataggtc aaaatatcat tgcaagtata ttaattattt tactatttat
    55201 gtggcagatt cctctttgta tgtttttagc ttcaaaattt ggtatgtttg ttacagttct
    55261 aacaaatatg ttctttacta ttgttggagt tgctgttttc tcaactacag aaatgtggta
    55321 tatgtttcca tattcgattc cttcaagatt aatggctgtt gtattgcata ttctgccaaa
    55381 tggtcttcct gtaccagagg gaagtgaatt tttaaataat gctgttattt tgccaggagt
    55441 attaatatct attgttttat ttgtattatt tgctttagct acaagggttt ggttcaataa
    55501 tagagaggtg aagtaatatg agacagttaa taagaatatt ttgttctgat ttaataaaat
    55561 taaagagaaa ttttataata ctaatgcaca tttgcattgc tttaatgggg atgggattgt
    55621 ttttatggta ttataaggtt tcaggagctg ataatatact aaagatagta gcatatttac
    55681 aagttatagc aatagctttt cctttattgt caagtgttgt gtgttcaatg tttgttgaac
    55741 aagaatatta tgcagggaat tataaacata tgttaacaac atctaatcca aaatatctta
    55801 ctcttataag taaatatata atcttagtat gtcttggttt tgtagcaaca ttagtgtctg
    55861 tattaggttt taaatttggt atatctaagg cttactttac atcaagcttt tatatgattt
    55921 caattctaat tttaattggg tgtaatttat ttgaatacat attacattta ttcttaagtt
    55981 taaggttcgg aaaaggaaca tcaattggag taggtatagt tgaaatgtta ctttcagcat
    56041 tattattaac tggattgggt tctggtattt ggcagtattt tccttgtgca tggggtgtta
    56101 gatttataac tatttggtcg tctttttcaa gttctaaaac ggttgaatat ataaaggttg
    56161 aatcaataaa agcatatcaa tcaacaggtt taatttgtgg ttttgtaaca attttagcat
    56221 ttgtaatttt atgtatttgg ttttctaaat gggaaggtaa aaaatcagaa gaatagagta
    56281 aaaattgact gtttatttat attattatgt taaaatataa cttagcaata aaaagatata
    56341 aaataatcaa ataaaaaact ggttgggtat taattatgaa ttaattaaac tattggagga
    56401 cagggtacaa atgcttggag gtaaaaaatc taacaacaaa aaagttgaaa agttaagaac
    56461 tatttttata aaatatttat ctttattctt tattatgaca atttcaattg tactcctttt
    56521 aatgttatca ttttctgtat tattatcatc aggtgtaatt ttaccagcta attatgcaga
    56581 aaaacaattt aataaatata aagaacagat tatttcaagt gaaaaagtaa ctgaagatat
    56641 aattccaagt atatatgagt atggggttta tactttggat gggaatttaa tttcagggac
    56701 ctttaataaa aaagaatcaa aagaagtatg gaatttaatg agagatattg aagaaaggca
    56761 tgcatattca gaaagttata taaagttttt taagaaagat gaagttttta taataaaata
    56821 caaaatagta tcagagtata gttctccaat tttaagagca tatttaccaa aaccagagac
    56881 attgggtatg attattttta gtataatatt ttttattgaa attgttatat tatcaaaagt
    56941 atttggcaag aaatttaata ttgaaatgga gcttttaaaa aatactacag aaaaaataga
    57001 acaacaggat ttagattttg ttgtagaatc tagtaaaatt tatgaaatta ataatgtcct
    57061 tttttctatg gataaaatga agttggcgtt aaaagattct ttagaaaaac aatggatgct
    57121 agaagaaaat agaaaggaac agatatctgc actggcacat gatataaaaa caccactaac
    57181 aattatacat ggaaatactg acttattaat tgaaataaat gaaaatcctg agttattaga
    57241 atacatggaa tatatctcaa agggtgtcac tcaaatagaa aaatatatta atactttaat
    57301 agatatatca aaaacagaga atggctatat attaaataaa gaagcaatca gtatgagtga
    57361 gtttataaaa gatgttttag tgcaaataga agcattagct agtactaaaa acttagatgt
    57421 ggagttttat aaacaagata atttgcctaa aaatataact atagatagag aactgttatt
    57481 cagagctatt atgaatataa tttcaaatgc tatagattat tcaccaagcc agagtaaact
    57541 atacataagt gtatcagtac ataataaata cttaaaattt gtagttacag attgtggaag
    57601 tggtttttct aaagcagatt tgtcaaaagc tacagaacag ttttatacag gagatttgag
    57661 tagaaattct aaatctcatt atggaatggg actctatatt gtaaataata ttgtacaaaa
    57721 acataatggg actttatgta ttgaaaattc tgtgaaaact ggtggtgcta tggtaacaat
    57781 tgaaatacca atagtttagt aaataatatc cattttgtaa atacctaaat tttatgattt
    57841 ataggtattt ataaaatgga tagttttttt gattttatat attggttaaa tctgataaaa
    57901 agttttcaaa cataactcca tcatttctat cagtatcaaa ttgagatgtg taatttatag
    57961 ttttatatat aaaatctgtt gcagtattaa ccgctactcc caaatcatgt ttatttacta
    58021 acataccaca aagtatagat gtgaatatat ctccagttcc actgtaagaa caattattat
    58081 atttgactga tgtaaagaat acatggtcat tatctctatt gtaagccaaa tttaatatat
    58141 tatcatcttc taatatacca gtaataacga ctttgctagg tcccaaatca gatacagatt
    58201 ttgcaatata tataagctca tctctattat aatcgctttt ggtataatca tttccagtta
    58261 agaaacaagc ttctgttaaa ttaggagtag ttaaatctga atgtttaact aattctttaa
    58321 tttcctgtct catttcttct gtgaatattg gatacataac tccattatca cccatcacag
    58381 ggtcaactac gataaaagca ttgggataat cgtttataaa attagccact atctcaattt
    58441 gatgctttga acccaaaaaa ccactatata tactatcaaa attgactttt aaatttttcc
    58501 aaacatttga atattttacc atttcatcag taaagtctaa gaaagtaaat tcaggataac
    58561 cagtttgact tgaaagtatg gccgttggaa atggacaaca ttgaaccttt aatgctgaaa
    58621 gtataggtat agcaaccgat aatgagcatt ttcctattcc agataagtca ttaattgctg
    58681 cgactttttt ttgcataaaa aaccctccat tagtatgtat taattataat gataccaaaa
    58741 tatttatata catatatatt actataaaaa aaagaaaaaa gtagattata aaataaaaaa
    58801 ttgtaaaaaa aacagtgtat tgatacaatt aatttttaat attggacata ataaaataga
    58861 gaattattaa atatgaaaat tactatatgt gacagaatat tctttgtaat ttgatttgta
    58921 aggtgtacta tttgggaggt gtagctttga aaatactgaa aaataagaat aagtacatca
    58981 aaggtataat ggcatttgta tttttgtctt tagctactac agcaggtgta tttttaatca
    59041 atgatttaag tatgaaaaat atggaagaaa aagtcatgga atctgcaaat gttgaaggtg
    59101 atgatgtaaa agaccaatat aaactattgc ttgaaaactt atttgactac agaaataagg
    59161 caatattaga gaaaaatgaa aatattttaa aaggtcttta tgaaacggat aaaaaatttg
    59221 gtctgtgggc ttatgaaaat gaagtaaaaa agatgaaata tttagaaaac tggtctagta
    59281 aacaaggtgt aaaatttaaa gacataaaga caagggttaa gattaaaaaa ataaaggaaa
    59341 aagaaaaaga cctctatggt ataatctgtt cagtatccac tgaatataag tattcatatg
    59401 aaaatcaaaa ggacaaagaa aatgccttta gaataggtac atatcattat ctaaatgtaa
    59461 aaattataga taatcaatat gtcataacta aagagtggta tacagaccct tttgcagact
    59521 ctttaaattt agaaaatata aaatctgatg atataagaaa ttatattggt caacaagatg
    59581 gtgtagagtt acagcttaca gatgaacaaa aaaaagctat agattatgca cataaatact
    59641 gtggagccgc agctgatgaa gaacatggta tgaaatttaa tccaaactat agggattata
    59701 atccagaagg tggagattgt gcaaactttg catcacagat aatgtttgaa agtggaaggt
    59761 tcaaaaagaa ctcaatttgg aattatagcg agagagcagg tacaaaagca tgggtaaatg
    59821 cacaagcttt taaaaattat gcactgtata gtggaagagg ttcattaatt gctaaaggtt
    59881 cctatgaaga agtatacaaa gaagcttata atttacgtcc aggggatttt gttggttatg
    59941 agaagaaagg cagaataaca catgtttcga cagtgacagg attagactca agaggatatc
    60001 ctttggtaac ttgccataat actgatagaa tgttggtgcc atgggattta ggctggagtg
    60061 ataagtccat aagattccat ataataaaaa taaattatta aaaaattata aagaaaaata
    60121 aagtgtaaaa gataataatc atttaaaatt ataaagtaaa aaaattgatt attaatgtat
    60181 aaaaataata ataattgaca aacatagcta tatatgatag agtagtaata aggttacaga
    60241 aaagagtagc taaatatttg tgttttttta ggaggattta ttaatgaaaa ctggtgtagt
    60301 taaatggttt aacaacgaaa aaggatttgg attcatatct gtagaaggtg aaggagatgt
    60361 tttcgtacat ttctcagcta taactggaga tggatacaaa acattagaag aaggacaaag
    60421 cgttgagttc gaagtagtag atggagctaa aggtcctcaa gctgctaacg tagttagatt
    60481 ataattaaaa attcatatat attagtgttc tagtaatatg agagataagc cgctaatcat
    60541 ttagcggttt tttcagtttt tgtaaataaa attaatagca aaataaatta aaattattaa
    60601 aataaaatca ttaataatga gataaatatg ttaaaattaa tagtgtagag taaaattatt
    60661 atggctaagt ttaagtcaat taatttttag taaattttct aaaaagagaa taagtgatac
    60721 ttatagacca tataaaaata ttaataaagc ataataagat taaaatgagc aaaggaaaaa
    60781 cgcttgttac agcgtatagt attaagaata cttagaaaat aagaaaggag aaagttactg
    60841 caaaaaagta acattataaa tattgagtaa tatttataag ttttcagtaa cggtgggtag
    60901 tgtaatgaac atattattct ttctaacacc aaaaagtgaa gtagcatata tatatgaaga
    60961 ctacactata aggcaagccc tagaaaaaat ggaatatcac aaatactctg caattccaat
    61021 aataagtaag gatggtaaat atgttggaac tataacagaa ggtgattttt tatggacatt
    61081 aaaaaatgat ttaaatctgg acttaaaggg attggaagat gttccagtga cagatataaa
    61141 tagaaaaatg gataattctc ctgtttccat caacgcagat attgaagatt tagttataaa
    61201 atctttaaat caaaatttca ttcctgttat agatgaccaa gacacattta ttgggataat
    61261 aaaaagaaga gatgtaatag gttactgtta tgagataata cgtggatata aaaatttagc
    61321 taatgataat taaaaatctt atttaaactt ttaagctgag ttctttacaa taaaaaaatg
    61381 ataatatgaa actgaatatt taaactttaa tataaaaagc tgtcttttct aataaatatt
    61441 gtgaaaagac agctttttta tatatgacct tatgattaat cgttgtattt tacaaggttt
    61501 ccgtacttag cattaagagc atttatctta tttccataaa cttcattttg tttttgatat
    61561 aataaacttt tttcaacttc agccttaact tcatcaaatg tagactctgt actttcttga
    61621 aggtcttcta attttattat atggtatcca aattgagttt taactggttc acttacttca
    61681 cctttagcca tagagaacac agcttcttca aattcaggaa ccatttgtcc tctaccgaat
    61741 gttcctaaat caccacccat atctttagat gggcaagaag aatgtttaag agctgcatct
    61801 tcaaaagata tttcttctga ttttatttga gcaagtattt ctttagcttt ttcatcacta
    61861 tctactaata tatgtttagc agttgcactt tctggtttag agaaagagct cttattagct
    61921 tcaaagaatt ttactttttc atcttctgtt acattaacac tagataaaac tttatttata
    61981 gcaaattgct ttaatacatt ttcttctatc tttttcattt ctaatttaaa tatttcttct
    62041 tcatctaatt tttcttcttt agcatctaag aagaataatt cttgattcac taaatcattt
    62101 aataagtgtt tttttccttc ttcagtttta aattgcatag cttgataagg gtctaatgat
    62161 tttaatgcat tttcaatatc aatattagtt atctcttttt ctccaactgt tgctaaaact
    62221 tttttttcca taatagttaa ctccttcata gtgtatttca ttattaatat aagactacca
    62281 tcttaaataa tatcagaaaa taatgtagat gtctaatatt taaagccata ttaagtcaaa
    62341 tagaattaaa tttagtagaa caaagttatt acaattaata aaagtgagct cataataccc
    62401 actacacctt cgtcatcttt aaatttaaga gtttttacta ttgaataata aaggtatatt
    62461 ccagtaaaaa taatctttaa atacaagaaa aataaactct ttgggtatat taaaaagcta
    62521 aaaagtgtaa atataaaagc aagtagagta cttatcctgt aaaagaaatc tatttgtgta
    62581 atgtctttat attttatcat aaacaccgaa tataaaatga tgaaaacaat tgcgacgatt
    62641 gcatatatgg taaataacat attttcaaga gtaccatttg aaattactat ccacttatac
    62701 cacataattg gccaaaataa tagtgctaag aacttaaaat aattatcaaa caacttttct
    62761 ttatacattc a
//
LOCUS       DAGSHT010000005        58828 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGSHT010000005 DAGSHT010000000
VERSION     DAGSHT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.006"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(181..375)
                     /locus_tag="MJB72_000257"
     CDS             complement(181..375)
                     /locus_tag="MJB72_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261435.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(684..2213)
                     /gene="lysS"
                     /locus_tag="MJB72_000258"
     CDS             complement(684..2213)
                     /gene="lysS"
                     /locus_tag="MJB72_000258"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBY8261436.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2228..2707)
                     /gene="greA"
                     /locus_tag="MJB72_000259"
     CDS             complement(2228..2707)
                     /gene="greA"
                     /locus_tag="MJB72_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBY8261437.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2816..3784)
                     /gene="dusB"
                     /locus_tag="MJB72_000260"
     CDS             complement(2816..3784)
                     /gene="dusB"
                     /locus_tag="MJB72_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBY8261438.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3921..4691)
                     /locus_tag="MJB72_000261"
     CDS             complement(3921..4691)
                     /locus_tag="MJB72_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBY8261439.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4710..5300)
                     /locus_tag="MJB72_000262"
     CDS             complement(4710..5300)
                     /locus_tag="MJB72_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY8261440.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5483..6451)
                     /locus_tag="MJB72_000263"
     CDS             complement(5483..6451)
                     /locus_tag="MJB72_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBY8261441.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6444..7421)
                     /locus_tag="MJB72_000264"
     CDS             complement(6444..7421)
                     /locus_tag="MJB72_000264"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBY8261442.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7736..9706)
                     /gene="ftsH"
                     /locus_tag="MJB72_000265"
     CDS             complement(7736..9706)
                     /gene="ftsH"
                     /locus_tag="MJB72_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBY8261443.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9740..11128)
                     /gene="tilS"
                     /locus_tag="MJB72_000266"
     CDS             complement(9740..11128)
                     /gene="tilS"
                     /locus_tag="MJB72_000266"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBY8261444.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVIEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(11311..11739)
                     /locus_tag="MJB72_000267"
     CDS             complement(11311..11739)
                     /locus_tag="MJB72_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBY8261445.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11756..12562)
                     /locus_tag="MJB72_000268"
     CDS             complement(11756..12562)
                     /locus_tag="MJB72_000268"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBY8261446.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12764..13249)
                     /locus_tag="MJB72_000269"
     CDS             complement(12764..13249)
                     /locus_tag="MJB72_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBY8261447.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13300..13959)
                     /gene="spoIIR"
                     /locus_tag="MJB72_000270"
     CDS             complement(13300..13959)
                     /gene="spoIIR"
                     /locus_tag="MJB72_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBY8261448.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14104..14784)
                     /locus_tag="MJB72_000271"
     CDS             complement(14104..14784)
                     /locus_tag="MJB72_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8261449.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14768..15658)
                     /locus_tag="MJB72_000272"
     CDS             complement(14768..15658)
                     /locus_tag="MJB72_000272"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBY8261450.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15776..17326)
                     /locus_tag="MJB72_000273"
     CDS             complement(15776..17326)
                     /locus_tag="MJB72_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBY8261451.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17420..17686)
                     /locus_tag="MJB72_000274"
     CDS             complement(17420..17686)
                     /locus_tag="MJB72_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBY8261452.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17834..18694)
                     /gene="yabG"
                     /locus_tag="MJB72_000275"
     CDS             complement(17834..18694)
                     /gene="yabG"
                     /locus_tag="MJB72_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBY8261453.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18778..19716)
                     /locus_tag="MJB72_000276"
     CDS             complement(18778..19716)
                     /locus_tag="MJB72_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBY8261454.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19826..19987)
                     /locus_tag="MJB72_000277"
     CDS             complement(19826..19987)
                     /locus_tag="MJB72_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261455.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19997..20494)
                     /locus_tag="MJB72_000278"
     CDS             complement(19997..20494)
                     /locus_tag="MJB72_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBY8261456.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20609..21319)
                     /locus_tag="MJB72_000279"
     CDS             complement(20609..21319)
                     /locus_tag="MJB72_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBY8261457.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21495..22025)
                     /locus_tag="MJB72_000280"
     CDS             complement(21495..22025)
                     /locus_tag="MJB72_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261458.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(22042..22272)
                     /locus_tag="MJB72_000281"
     CDS             complement(22042..22272)
                     /locus_tag="MJB72_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261459.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22287..23681)
                     /locus_tag="MJB72_000282"
     CDS             complement(22287..23681)
                     /locus_tag="MJB72_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBY8261460.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23807..25060)
                     /locus_tag="MJB72_000283"
     CDS             complement(23807..25060)
                     /locus_tag="MJB72_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8261461.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25111..26265)
                     /locus_tag="MJB72_000284"
     CDS             complement(25111..26265)
                     /locus_tag="MJB72_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY8261462.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26825..27394)
                     /locus_tag="MJB72_000285"
     CDS             complement(26825..27394)
                     /locus_tag="MJB72_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8261463.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27597..27824
                     /locus_tag="MJB72_000286"
     CDS             27597..27824
                     /locus_tag="MJB72_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY8261464.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(28043..28786)
                     /locus_tag="MJB72_000287"
     CDS             complement(28043..28786)
                     /locus_tag="MJB72_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY8261465.1"
                     /translation="MENYQSINTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28954..29826
                     /locus_tag="MJB72_000288"
     CDS             28954..29826
                     /locus_tag="MJB72_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8261466.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29826..30491
                     /locus_tag="MJB72_000289"
     CDS             29826..30491
                     /locus_tag="MJB72_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY8261467.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30512..31369
                     /locus_tag="MJB72_000290"
     CDS             30512..31369
                     /locus_tag="MJB72_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8261468.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31525..34071)
                     /locus_tag="MJB72_000291"
     CDS             complement(31525..34071)
                     /locus_tag="MJB72_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8261469.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENENLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34071..34757)
                     /locus_tag="MJB72_000292"
     CDS             complement(34071..34757)
                     /locus_tag="MJB72_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261470.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34840..35829)
                     /locus_tag="MJB72_000293"
     CDS             complement(34840..35829)
                     /locus_tag="MJB72_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8261471.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35816..36502)
                     /locus_tag="MJB72_000294"
     CDS             complement(35816..36502)
                     /locus_tag="MJB72_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8261472.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(37002..40208)
                     /gene="carB"
                     /locus_tag="MJB72_000295"
     CDS             complement(37002..40208)
                     /gene="carB"
                     /locus_tag="MJB72_000295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBY8261473.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40401..41450)
                     /gene="carA"
                     /locus_tag="MJB72_000296"
     CDS             complement(40401..41450)
                     /gene="carA"
                     /locus_tag="MJB72_000296"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBY8261474.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41707..44913)
                     /gene="carB"
                     /locus_tag="MJB72_000297"
     CDS             complement(41707..44913)
                     /gene="carB"
                     /locus_tag="MJB72_000297"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBY8261475.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44966..46009)
                     /gene="carA"
                     /locus_tag="MJB72_000298"
     CDS             complement(44966..46009)
                     /gene="carA"
                     /locus_tag="MJB72_000298"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBY8261476.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(46035..46751)
                     /gene="pyrF"
                     /locus_tag="MJB72_000299"
     CDS             complement(46035..46751)
                     /gene="pyrF"
                     /locus_tag="MJB72_000299"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBY8261477.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47127..47762)
                     /locus_tag="MJB72_000300"
     CDS             complement(47127..47762)
                     /locus_tag="MJB72_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBY8261478.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47786..48700)
                     /locus_tag="MJB72_000301"
     CDS             complement(47786..48700)
                     /locus_tag="MJB72_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261479.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49301..50530
                     /locus_tag="MJB72_000302"
     CDS             49301..50530
                     /locus_tag="MJB72_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY8261480.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(50735..53116)
                     /locus_tag="MJB72_000303"
     CDS             complement(50735..53116)
                     /locus_tag="MJB72_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBY8261481.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(53088..53813)
                     /locus_tag="MJB72_000304"
     CDS             complement(53088..53813)
                     /locus_tag="MJB72_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBY8261482.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(53803..54258)
                     /locus_tag="MJB72_000305"
     CDS             complement(53803..54258)
                     /locus_tag="MJB72_000305"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBY8261483.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(54540..55721)
                     /locus_tag="MJB72_000306"
     CDS             complement(54540..55721)
                     /locus_tag="MJB72_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY8261484.1"
                     /translation="MKNKSLLNHMFRPILDIIISCALTSMLILTSMYIIKSLYFNSGI
                     SVINQLVYQLRDLRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(55711..56403)
                     /locus_tag="MJB72_000307"
     CDS             complement(55711..56403)
                     /locus_tag="MJB72_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8261485.1"
                     /translation="MNNTILVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(56537..57253)
                     /locus_tag="MJB72_000308"
     CDS             complement(56537..57253)
                     /locus_tag="MJB72_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY8261486.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            57378..58112
                     /locus_tag="MJB72_000309"
     CDS             57378..58112
                     /locus_tag="MJB72_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBY8261487.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(58397..>58828)
                     /locus_tag="MJB72_000310"
     CDS             complement(58397..>58828)
                     /locus_tag="MJB72_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8261488.1"
                     /translation="RQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKY
                     LESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRK
                     YTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
BASE COUNT        18451 a        10866 c         6496 g        23015 t
ORIGIN      
        1 tatgtttgcc cttttcttaa ggacaagtaa tactatatca tcttaaaaat gtttcgtcaa
       61 tacttttttt gttttttatt ttaaaaaagg ttataacttt tggttttttt aataaatata
      121 attaaaacta atagatataa cccttgatat tacaaacaat acatgcatat ataattttac
      181 ctattttgca attttatttt cataagaata tgaattagct ctcactctat ctgcccaaac
      241 atttatactc ttaactttat taacattagt tgtaccctca aaaatatcca ttgcatcctt
      301 acaaaaatat atcataacat caagaaccaa aaatccaaca caaaataaac ataaaaaatt
      361 aattacttga aacatcagac attcccccaa taatttttta tatatagtat tctcataaat
      421 tattcttttt accttctcat aaattattct aaatattttc ataataaatt actttcaatc
      481 atattttcta aatataaagt ctcaatcaac aaaaactaat atcaatttct aacgtttgtt
      541 ggtaatttat aaattaaata tctaaaagaa tctttgaagc atataaaaag cattccacac
      601 tgacatttta aataaaattt agctatttat tgataattta atagctaaac tttatttaaa
      661 acttagtaga atgctaatat ttcttagttt tcttcttctt tatttggatt gttatcaata
      721 ggtttcatag tcgggaataa taatacatct cttatagatg gagagttagt taataacata
      781 attactctat ctatacctat tcctagtcct cctgtaggag gtagacctac ctctaatgct
      841 tttaagaaat cttcatccat ttcaaaagct tcatcatcac ctaattctct ttttctcaat
      901 tggtctataa atcttccttt ttggtcaata gggtcattaa gttcagaaaa tgcattagct
      961 aactcccact tattagcaaa tgcctcaaat ctatcagttc ttcttgggtc ttcaacattt
     1021 ctcttagata atggagatac ttcaactgga tggtgagtta taaatgttgg ttggattaat
     1081 ttatcttctc caaattcttc aaagaaagca tttattactt ctcctcttct cattccaggt
     1141 tttatctcta tacctttttc tctagcaact tctagtgcct cttcatctgt atttatagta
     1201 gaaaaatcta caccagaata ttctttaaca caatcttcca tactcattct tttccaaggt
     1261 ggtgtaaagt ttatttctgt tccttgatag ttaactatca tacttcctgt agcaacttca
     1321 gccatatgtg caataacatt ttctgttatt tccatcatat cagtatagtc tgcatatgct
     1381 tgatataatt ctattgcagt atattcagga ttatgtttta aatccatacc ttcatttcta
     1441 aacattcttc ccatttcata aactttatca aatccaccaa caataagtct ttttaaatac
     1501 aattcattcg ctattcttaa atacattggt atatgtaaag tattatgatt agttataaat
     1561 ggtctagcat ttgccccacc tgctatagta tttaatattg gagtctcaac ttctaaaaat
     1621 cctctatcat ctaagtatgc tcttaatgct tttaatgctt ttgttctaat taaaaaagca
     1681 ttttttactt ctggatttac tattaagtca acatatcttt gtctatatct tagttcttgg
     1741 tcttttaatc catgatattt ttctggtaaa acttgtaaag atttacatag taatactaca
     1801 gatttagcct ttacagatat ttcacctttt ttagttttaa agatttctcc ttcaattcca
     1861 actatatctc caatatcata tgttgaaaat acactatatt cttcctctcc tatagcatct
     1921 agtcttacat aagattggat tcttccctct tggtcttgaa tatctataaa gccagctttc
     1981 ccttgtattc tcttgctcat tatacgtcct gctatttttg tagttttacc ctcaagagaa
     2041 tcaaagttat cttttatatc catagaataa tgagttctat catatctact ttctttaaaa
     2101 ggatctctac ctgactcttg taattttttt agtttatctc ttcttacttg aagtacttca
     2161 ctaaggtctt cttgaatttg cgcttcttca ttgctttgtt gattattttt catttttttc
     2221 ctccaaatta tcttcttatt tctaatattt caaacttagc aactccatca ggaacttgca
     2281 cttccacaac ttctttcgca gctctaccta aaagtgcttt ccctacagga gattcgtttg
     2341 atattttacc atcatatggg tctgcttctg tcgaaccaac tatagtatat tcaacctctt
     2401 catcaaattc taaatcctta actttaacta ttgatcctat tgtaacaaca ttaaggtcta
     2461 ttttgctttc atctatgata acagcttttc taaccatatt ttctaatttt aaaattcttt
     2521 cttcaacttg tgcttgttcc tttttagctt catcatactc agcattttca gataaatcac
     2581 caaaagatat agcaactttt attctctctg caacttcttt tctctttaca acctttaagt
     2641 tttctaattc ttcttctaat ttatcatatc cctcttgtgt caataaaaac tctttatttt
     2701 cttccattat aaatacaact ccttcacaaa tttcttagga attttcctaa ttatatttta
     2761 aaaattttat aatctttttt taatttatgc gctatattat aagtcactat tatttttatg
     2821 tcaaggagtc ttccatatag tactccaatt tcgacacaac ttcttgatag tcttctattt
     2881 tgttaatttc atcccttaat tttgcagagt ttctaagtcc ttttaaatac caagcgatgt
     2941 gttttctcat ttcccttaca gctacatact ctccatgttc ttctactgca agttttaaat
     3001 gcttaatggc agtacttatt ttttcgttaa gagttggctc tggcaaaatc tcgcctgtat
     3061 tcatataatg gtttattctt ttaaatatcc aagggttacc ttgagcaccc ctacctatca
     3121 ttatagcatc acaaccagtt ttgtctaaca tatttattga atcttctatt gtaaatacat
     3181 caccatttcc tataacaggt atactcaaat ttttcttaat ctctgcaatt atatcccagt
     3241 ctgctttgcc tgtataaaac tgttctctag ttcttccatg tattgctaaa gcacttattc
     3301 cacaatcctc tgctatcttt gctatttcaa cagcatttac actattatca tcccagcctt
     3361 ttcgaatctt taaagtcact ggcttttttg agttcttcac tactgccctc aatacctctt
     3421 ctgcaagctt aggatttttc ataagtgctg aaccatctcc atttttaact acttttggag
     3481 ctggacaccc catatttata tcgagaatct catttgaata atcattcatt atctctgttg
     3541 ctcttcccat aaactctggt tctgaaccga atatctgaac tgctacagga tgttcttcct
     3601 cttctatctt aagcattttt ttagtatttt catcatcata gcaaagtgcc ttaccattta
     3661 tcatttctgt atataaaagc ccacatcctt gttctttaca aattaatcta aatggaaggt
     3721 ctgttacacc tgccataggt gatagaaaaa ctttattttt caattctaaa tttcctattt
     3781 tcattgtttt tctcctattt ttgcctttat tttgtgattt aactttaaat tcttatttcc
     3841 tcattataat ataccacata tagctagaaa tctatcataa aaagggaatt ggatatataa
     3901 cccaattccc tgtatattaa ttattcttgg tttttttcat atattattct caatccttct
     3961 agtgttaaaa aaccatctac ataatctata cttttcgtct ctgaagcaat cagtttagct
     4021 aatccacctg tagctataac cttaacatca tcacaattca attctttctt catcatactt
     4081 attattttgt caactaaacc aacatatcca taaattattc cagattgcat ggctgataca
     4141 gtactcttac aaatagtcat acctggctta gctaattcta ctctaggtaa tttagacgca
     4201 ctttgaaata acgcctcact agatattttt attcctggtg ctattgttcc acccaaatat
     4261 tcaccttttt cagagatagc acaaaatgta gttgctgttc caaaatcaac aagtatactt
     4321 ggtgctccat acttttctat ccctgctaca gcattaacta ttctatctgc cccaacttgt
     4381 tttggattat catattttat atttagacct gtttttatgc ctggacctac tattaatggc
     4441 tgttttttac agtactttat acaaaagttt tcaagagaat gcataacatt cggaactaca
     4501 gatgatatta taacatcatc aatatcactt atattcacat tatcataatc aaataggtta
     4561 cttatcagga ttccatattc atcagacgtt ttttccctat ctgttttaat tctccagtaa
     4621 ttaactaatt tgtcaccttt atatatacct aaaaccatat tagtatttcc aacatcaaat
     4681 actagaagca tatgtcatta accttctttc tattttttgc ttttcttaag tacagtggca
     4741 acagcagcta ctactaaagc ccctactatt gcttctggca ccccatttgc tgcggcaaga
     4801 gccattatta cttttcctgc tgaaccactt ttacccattg catgaagata cttgtcagca
     4861 tataagacat atatcattcc taaaactcca gctgtattta acagagaacc tattgcccct
     4921 gtaattaaac ctgatatgta taccttattt gtagccttaa ttattaactg atatacataa
     4981 taagctaata cacctatcat aactcttggt aatattgaaa ctaatggatt tataaaaaca
     5041 aaagatgttg ctgttgggtt tgttatagca tttaagagac ttgatatccc aaatataaat
     5101 cctattgctg ccccaactat tggtccttcc ataatagctc caataataac tggtatgtgc
     5161 attatagttg cttttgtagg tccaactgga ataaatccca atggagtcat agatagcata
     5221 atagaaatag ctgatagtac tcctattata gtcatctttc ttacatttac ttttcttact
     5281 gatgcacttg tgttcatcat aaaaaataca cctccgttcc agctcattaa gatgccggac
     5341 tacttaattt tttaattttt ttcgtttttt cttataacta tgagtcaagc taccattgta
     5401 ggatgcccgc tctaacaaga ataattttat caagatgagt tgccattgtc aacaatttga
     5461 caagaaaaat atgctaattt catcatattt ctcgtttttt tactatatct ttatatgcca
     5521 ttatattttc tacactatcc gcttttttta ctgcatttat tatacttttt tccacaacct
     5581 ctacagccaa tgaacctact aatgttatat ctgcttctat ttctcctgtt gatattgtga
     5641 atattgtatc tccatcatga ggtgtatgga taggaaaaat agacttagca tatccatcat
     5701 gtcccatttg agatatcttt ttacatccag ctttggttaa cttagcattt gttgcaatta
     5761 tcccaattgt agtattatct acactaaatc cacctttact ttcaccttgt ttcattaatt
     5821 tatatgtatc aattgtttct gtctttttat catttaaagc accagctata accttaccat
     5881 tttcataaac atctccaaga gcatttacag caattaatgc agatacaatt agaccattat
     5941 ctaattttat agaatagcta cctattccgc ctttcatagc atattcctga cctcttatct
     6001 ttccaacagt ggctccacaa cctgctccat aatttccttg atttaaaata tcagtatttg
     6061 caacttcaca agctctataa cccatcttta aatctggtct acatttaaag ctccctactc
     6121 ctaaatcaaa taaaactgct cctacaacta taggcacttt acctacacct acatcaaagc
     6181 caattccttt ttcttctaag tatctactta caccacaagt ggattcaaga ccatatgcag
     6241 aacctccagc caaaactact gcatgaactt tttgaaccat atttacaggg tctaataaat
     6301 cggtttctct agtacctgga gcagagcccc ttacatcaac accgcaagta gctccttctt
     6361 cacatattac tactgtgcac ccagtaagac catttctatc ctcaacctgc ccaaccttta
     6421 agccttctat atccaaaata ttattataca tatccttttt ctcctcttat agaaacttca
     6481 cctgaaataa tttcttttat acttccatca cttctttgaa ctaataaatt tccctcttca
     6541 tttatgtcta aacatttcac tagttctcta ttatctcctt ttaatacata tatattttta
     6601 tctataattg cagaataatc tctacatatt tttaaggttt tttctttgga attatttaca
     6661 gtataatcta aatataattt ttcaaattca gttaatacat tcctaacaat atctactctg
     6721 gataacatat atccttcttt atacaatgat gtggctttgt cagctatttc ttgttcaaaa
     6781 tccattattt taacatttat tccaattcca agaacaacat aatttgttct ttcaatttca
     6841 gcagataact ctgttaatat accacatatt ttttttccat ttaaaataac atcatttggc
     6901 cattttatta atgtatttgc tcctagattg tttaaagctt tcgttacact tgctcctgct
     6961 attaaagtta taaatggggc tttgaatggt tccatatctg gttttaatat aatactcatc
     7021 cagattcctt catgaggttt tgaatcccat cttcttccca atcgtccttt ccccatatct
     7081 tgatgttcac ttaaaatcac agtcccatct acactattta tagctatcct tttagcatgt
     7141 tcatttgttg aatctatagt gtcaagatgt tcaatatttt ttccaataaa ctctgtatct
     7201 aatttatagt aaatattctg tgggcttaat aaatcagttg gattttccgc taatcgatac
     7261 ccttttttat ttacagattc tatattatat ccctcttctt ttaaggcttt catatgtttc
     7321 catatagctg cacgtgacac ccctaattgt tttgataatt cttctcctga aataaattca
     7381 gatttattat ttaatataat ctctattatt ttatctctca tatttcacct ctaatatgac
     7441 ataatttcat gacttatttt cttctaataa ataattatac attattaata tacttttaat
     7501 actattaatt ttaaatttcc tttttaatta ttataatggt tgttttatat tttatattga
     7561 tgatttatat aattttacat tttatttaaa taattttaaa ataataaaaa aactgaaagt
     7621 atcaattaaa ttaatacatg caaatataat ttgcttatta atttaatatt atactttcag
     7681 ttttagtatt taatccacat cagataataa tcttaaaatg accaaggtct taaaactatt
     7741 tatctttatc agatttatct tctaaatttt tatttatatc aataacattg tttctttctt
     7801 cttcatcttt ttctaatttt atagttaatt gttcttcaac ttctttagaa gaattttcct
     7861 ctgtaactgc attttcaatt tcattcagag gtaactcttt attaaatgct tttacaaatt
     7921 gttctgcatc taaagtttca tatataagta aagcttgtgc tacatactct aatctatcca
     7981 tattttcttg taatatactt cttgtcttat catatgcacc atcaactata cgctttgttt
     8041 cttggtctat ctcaaaagca acttcttcag aataatttct cttacttgaa aaactattac
     8101 ctaggaatac ttcatcatca ctgtcaaatg acattggacc aagtttagaa ctcattccat
     8161 actttgttac catacttctt gcagtagctg taactctttc taagtcattt gaagcacctg
     8221 tagatacatc cttaagtact agctcttctg ctactctacc accaagtaaa actataatat
     8281 tttctttcat ttcatttttt gtagcataga acttatcttc aacaggaagt tgcattgtaa
     8341 accctccagc tcttcctctt ggaactattg taacttgatg aacaggactt acttcttcaa
     8401 gtacatgggc acaaactgca tgccctcctt catggtatgc tgtaagtctt ctttctttct
     8461 cacttattac tttcgatttt ttagccacac ctgctataac ctttgttata gcttcttcta
     8521 tagtttccat cttaattttc ttttctcttt ttctagctgt taatattgca gcctcattca
     8581 ttaagttctc tatatcagca ggagtaaatc ctggtgttct tctagccaag acatccattt
     8641 ttacatcatc acttaatggt ttatttcttg agtgaacttt aaatatagcc tctctacctt
     8701 ttacatctgg tgtaccaaca acaacttgtc tgtcaaatct tcctggtcta agtaaagctg
     8761 ggtcaagtat atctggtcta tttgtagctg ccatgattat tataccttga tttaccccaa
     8821 atccatccat ttcaactaga agttgattaa gagtttgctc tctttcatca tgacctccac
     8881 caagacctgc acctcttttt ctaccaactg catcaatttc atctatgaat atgatagctg
     8941 gagcactttt cttagcctgt tcaaataaat ctcttactct tgaagcacca accccaacaa
     9001 acatctcaac gaaatcagaa ccacttatac tgaaaaaggg aactcctgct tctcctgcaa
     9061 ctgctcttga tagatatgtt ttaccagttc caggaggtcc taccataagc attccttttg
     9121 gtattcttgc acccaattca atatatttct taggattctt taagaaatct actacctcct
     9181 gcaaatcttc tttttcctca tctagtccag ctacatcttt aaatgtaact ctagtttttt
     9241 catcatcttt atgaacctta gcttttgatt tgccaaaact cataaccttt ccgcctccac
     9301 cttgagactg attcataaac acgaaccaaa taatcaccat gaagaatatt aacagtaatg
     9361 taggcaacat ttcgacaaac caaggtgtag atggcttagc ttcaccacca aaagttagtt
     9421 tcccagcctt agcttggtct aacacctcat ctgcaagttt atttcccatc acttctgttg
     9481 gaacatatga cttaaacttt gtatttgtat cttttatagt accttctaca gaagtttgat
     9541 ttacaaagta taatcttgat atattctcat ctgttaattc tctatatact tcagagaatt
     9601 ttaaatcttt tattttttct gtaggcttac ctgagaattg aactatacct actataatta
     9661 taaaaacaag tagataaaaa cctgcccctt ttagcaactt attcaatata gaagccctcc
     9721 tttcatacat tgctttgccc tataatttgt taaaattaat ttttaatact tcttttgtgc
     9781 tttcatcaat tttataattt tcactgattt tataatctcc aacacatatt attccttcac
     9841 catctgtgat tataggaatt ttacatcttt cttctcttgg gatttttaaa tctatgaata
     9901 aatctttcac ttttttactt cccatagcaa gttttatctt atcaccttgc cttctgctcc
     9961 ttattactat accccctttt accttattaa aatcaaaccc tttagatgaa ttgtctaatt
    10021 tcatactctt atacctatct atactcatta cttgggtctc aataatttgt tttaattctt
    10081 ttattttaat aaacccattg ctaggtacat tgtaataaaa ttcaatttcc tcaataacaa
    10141 tttcctcatt ggttaatatt atactgtctt tttttctata aacaaatata tttcttggaa
    10201 gagttaacat tttatttact tttgaattat cttctaaaga cattatatct tctatatgtc
    10261 tttggtcaac aaaattagta tctcctagta tatgttttat agaatttcta agaaccctaa
    10321 ctttgatagc gtcatgtaag caaacaaaat cgtctaaatt tatctctaca aagccttttt
    10381 cttttatatt tgaaacttct ctaaatttag cttctgcctc tttctcaata taatcgttat
    10441 cactttttaa actattactc atcctcacta tagattctat tacatttgaa ttaaaattat
    10501 ccttcatata tggtaaaagg tctaacctta tcttatttct agtgtatata ttttctaaat
    10561 ttgttttatc tatcctcgga tttaaattat aagcttcaca atactcctct atctcatttc
    10621 tttccacgtc taatatgggt ctaattatgc aattatctct aatataatca attcccttta
    10681 agccttttaa acctgttcct ctcatgatac gcatcataac cgtttcggct tggtcattga
    10741 gattatgacc tattgctatt ttattggctt taatcttatc cttaatttca taaaacattt
    10801 catatcttag cttccttgct ccttcttcta atgataatcc ctcattttca caatattttg
    10861 gtacattaat agactttaca aagaatataa ttcccatatc ctcacaaagt ttagatacat
    10921 ataatgcatc tttttgagct tctatccctc ttatttgatg gtttaaatgt gctgcatata
    10981 tttctatatt aaaatctttt tttaatctat ttagtacatg caataaacaa actgaatcag
    11041 gtcctccaga aagacctaaa actattttat cgcctttttg tattaaatta tgtttattta
    11101 tagtacttag taccttatca aatatcatat ttatccctct tacacttagt tttattcact
    11161 tttaagtata ctttctataa aaaaacgcta aacccttgta tcaattttaa gaaattaaag
    11221 tttaatataa atataataat accaaataaa ttaaataaaa ggtagttttt tcactacctt
    11281 tataaaattt ctggaaaata gccatagtta ttatttagta cctatgtata tatctatttt
    11341 attcctacct aaaaataaat cttgtacctt tatattatag tctattttca attttatgtg
    11401 ttccccttct tgtatattta tatctaattc tcttgtatca atatcaatta ataaagtacc
    11461 ttgtggggtc acatactttg tagtactact ttctccacat ttaaacagca ttgttgagtt
    11521 aatagcacca aactttttga tactaacttg atttttaagt atctttatag tattagttat
    11581 ttctatattt tcttcttttt ccttgtaaat aacatatatt ccatcatttt tataaaaaat
    11641 tttaccaaag gcagtcatct ctatggtgtc catatttcct ttttcatcat attgtctagt
    11701 atttatactt atttttacac tcaacgattt ttcctccata gattcttcat ataaactagt
    11761 atttttgtat ttctactttt tgaatcttat caattttttt ctttaagaac tctcttgcta
    11821 tatctgaaaa tttttcaggt atatctgata cataatattg ataagttcca tgaatttctg
    11881 tattgtttat aatattttgc acctctaaaa tcttttttaa atcctttgca gtctctttag
    11941 ctggattaac aagttttata tgctctccaa cttcttcacc tattgttctc tttaaaatag
    12001 gataatgagt acatcctaac actaaagaat ctatattttt ttctttcaat tcttgtaaat
    12061 atatctttgc tgtcaattta gccacttctg tatttgccca tccttcttca acaataggta
    12121 caaataaagg acatgcttta ttaacaattt ctatactctc atctatttta gatatttcca
    12181 agttgtatgc ttttgaactt atagttcctt cagttcctat tattcctaca attttgtttt
    12241 ttgtagaact tacagctgtt ctagctcctg cttctataac tccaattatc ggtatatcat
    12301 atttttcttg agcttctttt aagcttcttg cagtagcagt gttacatgct ataactatag
    12361 cttttacacc ttttgttttt aaaaagttta tagcttgaaa agtatattta attatagttt
    12421 ctttggatct tgaaccatat ggtattctgg cagtatctcc aaagtatatt atatcctcat
    12481 ttggtaatat cttcattatc tcttttagaa ctgttaatcc acctaatccc gaatcaaaaa
    12541 ctcctattgg tttattgttc atctttatac aacacctact ttacattttt gtgtttaaca
    12601 acaacttatt tatcgctaaa atttatattt atattcataa ttaatatatt attacctaaa
    12661 tgattattat agtttatttt acacaaaaaa taaaggagct atcttaaatt agaaaataat
    12721 ttctacaaaa agacaactcc tttcactact taaaaattta aatttatttt gcaaacacat
    12781 gatttcctat tctcatataa ggattcaatc tatttatcca tttgcaagtg gctatttctg
    12841 gattccaaaa atatactgct ttattagttg ggtcagttcc ataaagagct tcctgagctg
    12901 ctttacgtgc tgagtctgtt ggtgccatct ttattgaacc atcaagcaca actgagaaag
    12961 catttttttg atatatgact tcttctattg tgtctggaaa tctagggtcc aatactctat
    13021 ttattactac agctgctaca gcaacctgtc cctcatagct ttctcctcta gcttctcctg
    13081 caaccaactt agataataac atcaagtcat tatcagataa atttattact tctttctttg
    13141 ctatattttt ttcttgtttg tttactttct taatattttc tgttggttca tatgcaaata
    13201 cctctttaat aggcataaat ataaaagtca aagctaatag tgtagccatc agtttactcc
    13261 acctgtccat gattaaaacc actccttaca ttcttatttt taaaattttt tctctaaatc
    13321 cttgactact tcaagtactt taaacttaat ttttactcta cttgtttttt ttggtgtatc
    13381 ttgttttatt aacttatatt ctttatcagt taaaacttcc ttcaacttat catctgtaga
    13441 tttatctatc actccattag attcatcaac aaaacatagt ggagggaaca taacacacca
    13501 ccagtttttt ccttcacctt tcccaataat aatctttaat gctttatatt caccagctgg
    13561 aagaactata ttagaatatt gtttagtagg gaaattgcta tattgtatac ctacctttac
    13621 actgtaatta tatccatttt taagaataat atttttactt atttcttcca gactactata
    13681 ttcctttttt ataatagctt cactttcttt tatacttttt gaattttgaa gctttggctg
    13741 taaatatttt attacttcat ctctaacttt tagtttaagt tcttggtctt catctgtatt
    13801 gctgtttgct ataacatgaa atcttattaa tttatcttta taatcttttg atatattgtc
    13861 tacttttttt acctcaccat ttattactat tgtcattatt gatatgactg atataagact
    13921 taatattaat agtcccaatc taatcttaat ttttctcatt gaaaattccc cctcaagata
    13981 aatttatatc atcaattatg tccaattaaa tttaatttat tcataatatt ttttaatact
    14041 aataaaaaaa gctatcttaa aatgattaca atcattttaa aatagctttt taaaccttat
    14101 attttataag tctttactaa tcatagcctt atctatcata aactgctctg ctttttctat
    14161 atgctctgta gcatacttct tggatagctc actatcacct ttaattatag catcaagtaa
    14221 atatttatgc tcttcaacaa tattatgtga tgtatcataa gcagacatat aggtaactct
    14281 aaatctatat acttgttccc acaatgagtt tattaattgg attaattttt tattattagt
    14341 tgctttaaat atacattcat gaaattctac atcaagtttt aataacgcat ctacatcgga
    14401 ttccaaagtg ctttttttaa attcttctga tatatcttta agctttttaa tatcttcatc
    14461 actaattctt tcagcggcaa gatatgctgc taagccttct aggcttgctc ttacttctaa
    14521 tacatccata atatccttta atgacatatt tgccacatac gcaccttttc taggcaacat
    14581 aacgactaaa ccttctaatt caagctttct tattgcctct ctcactggag tcctacttac
    14641 tcctaattgt tctgctagtt gcacttccat tagcctttga ccaggcttta attttccttc
    14701 taagattgct tctcttaaat tctcaaagac tacatctctt aatggcttgt aattatccaa
    14761 gttcagttta gttaaattct ccacaaattt caaccccttt ttgactgcta ttaacaacat
    14821 acacctgttt atattttttt aataattctt tttttccaat tagagcatct tctttatttt
    14881 taaatagtcc aaaaactgta ggaccacttc cactcatcat agaacctaaa gcattatttt
    14941 tcatcattac ttgttttata tcacttattt ctttgtgttt acctatagta acattttcaa
    15001 gaatatttac catgctttct gatacagctt ttatatcttc agattttaaa cactctatca
    15061 aatatttatt atttggtctt tttttaatat tattcaaatc taatccttga taaacttctt
    15121 tagtagaaac gaataagtct ggtttgcata tcaatatatt tgtatcacat ggtaaacctt
    15181 tgatatttgt tagcttttct ccaatacctt gagctaaggc aggtcttcct gatatacaaa
    15241 aaggaacatc tgcgcccaaa ttaagtccta tttcttttaa ttcatcttca gataatctaa
    15301 gttcccatag gtgatttaaa cctactaata ctgcggctgc attagaactt cctccagcca
    15361 tccctgctgc tacaggaata tttttttcta tgaatatttc tacacctttt tttatataaa
    15421 atttattttt aagtattttg gcagctttat aaacaatatt atcttcatct aaaggaatat
    15481 ctaaactagt gctttttacc tttatttcat cttcgtctaa ttcttttatc tttactatat
    15541 cgtataaatc tatcgtttgc attatcattt caacaaaatg ataaccgtct tgcctttttc
    15601 ccaagacatc aatggataaa tttatttttg ctctactctt tagtcttata aaatccattc
    15661 tatcctcctt gtttaaagaa taatgcatca ctaacaataa tattatacta caaaaaataa
    15721 aataataggg tattataata ccctattata cttaattact ttaaattttt taatattaat
    15781 ctactaatac gacttttttt tctaaaataa gacacttccc aggcttaatt ggttcatcaa
    15841 gttttatatc attaagttct gcaatttcat tttctgttgt attgtatttt tttgctatat
    15901 tccagaaagt atctccttct ttgcatatat atacgattat gctaggagct ttagataaat
    15961 catatacacc ttggtcttct cccttgataa taaaattttc tgctttctta tctaatgctt
    16021 cagtaaatct cttaattttg ataatcaagt ctatctggtc cctattcaaa tcaacttcga
    16081 ctttatcaat acatgctgta ttaaacacag atgctgtatc agttagatta tccatttcta
    16141 tatcatgttc aaatggaatt tcctcactaa ttttataaac caattttaaa ccttcaactg
    16201 ggacaaataa aatttcaact tttatgattc cttgtataat gcttttattt ccttcaatat
    16261 aactattttc tatagacatg gttggacaaa cgcttacaat atcttttatt tgtatatcat
    16321 cattatcatt tcttatgcct tctctaacca taaatgtttc ttcactacgt cttaaagtct
    16381 tatttaattg aataggtttg tggtcaaatt ttattatctt ttgaggcgaa tatgcatctt
    16441 gtaatacttc tcttgtaact tcatctgtaa cttttacctt acaacaagcc atacaatcta
    16501 tttctaaaag acctgtgttg ctttcactat tttgcttgaa aatatgatta aaatctgaca
    16561 tagacaacaa tacttcttca gtcataccat cactaactcc agggacttct acaaattgag
    16621 taaactcaat acctactcta tccaactcta caagttcacc ctcatatgta caagctaacg
    16681 gatttatttc taatactcca ccaataataa ctttattgtc agtcactcta ctttctttta
    16741 ctttaacaca tggatttaaa cttattatag attgaatttc ctctgtgttt attgttatag
    16801 tatctcttat agaactttct gctttttcta ttccaactat atcttgaaaa caaatttcct
    16861 ttctgtgctt ttgtatgcct tccacttgcg ccacatcttt tacaatgtct aacctttgct
    16921 tttcaaacaa acttcccctt atattcataa gagctcctac tttaatcttt ctctcattca
    16981 ttatagtaca atccatatgc tctacttctg aaaataacat gtattccata tcttgaataa
    17041 cattgtcttt ttctattact tcgtttatat ctacttttcc gtctacatta gaaatagtat
    17101 ttttatcatc tgcaatatag attacattat aattaaaact acctctgcaa agtatcttcc
    17161 catctgccat ttcaattttt ttaagtgata tgtatccttc tgtttttaca atttcatata
    17221 catctaattt tttgtctggc acaactgcct ccgcttcaat gaatgtttga aattttccaa
    17281 agtctattct attgtcaact ttaattacat ctttaattaa ttccatagtc tacctcctta
    17341 agtattataa aaatatcaca ctatatttat atttaaggag caaaaaaaaa attacaccta
    17401 tttttaaata ggtataattt taacttattt gtaatttatc ttgatctcta aaaacttgca
    17461 actttacatt agatgttaat aaatctgagt aactataaga tactctagga tatccattac
    17521 tttcatcatc taactttata acaaacacgc ttggataaac tttttctagt ataccctctt
    17581 ttgtaataat ctgctttctt cctttattag ctttcaacaa tattttcttt cctatatgtc
    17641 tctctaagct tactcttatc ttatctagag tttgaacagt agccacaata tcaccttctt
    17701 taacgtttat atgtttacat aatatcacat ataaacattt ttgtcaaatg tttaaactaa
    17761 ttattttata tattttttgc aaaaatgtca atactatttt tgaagatata cagtataatt
    17821 atgttaattt ttgctaatgt aatattgttt ttggcattgc ccatctaaat tttcctcttg
    17881 tttcagaacc accaattccc ttaacacctg taatagtctg ctctataacc tcatcaatag
    17941 gaactactac atcaattctt gaacaagcaa ttttctccac tataaaaaca gggtctaatg
    18001 catgtatcat tattcttcca ggcgaacttg catagtttgc tcctgcactt attatttttt
    18061 cataatttga ttggcatgct cctgcgaata tgacaagatt atccaaattt ggttgccatt
    18121 tacgtgcttc ttttactgtt ttaacaaaat ttgaagagtt tctatagtta ctcatatctt
    18181 ctatatctcc tctttttatt gtcattgcat catgacctgt tattactagt atatccggat
    18241 tatatttttc tagtagtgct cttacttctt tatactgatt ttgctctgaa acagctacac
    18301 ctactgcagg tattccaagt tgagcatata catccaagca aatttttaaa tattctttat
    18361 ctccatctat ctgtagtact tttccaggca ttccatatgt gttaggattt aattgaagtt
    18421 ttggaactgc tctagtcatt cttttttgtc tttctttagc ttttcttact gacttatata
    18481 ataaattttc tacattttta tctattaata tatcttttat atcaggtgct tttactaatt
    18541 ctaggtcatc aatataagca tctgctatta ctctaaatgc aattcccttt agtatagcta
    18601 ttttctcatt attttcatct acaccaaaag agactatttt aaatactata tctttgttat
    18661 gtgactttct agctacaatg tctcctacct tcatagttaa acctccaata aatttttatc
    18721 ttagtttata ctatgttcga tataaaactt tgtttcctaa tgtcaaaaaa acatttctta
    18781 taatacttct tttatcactc ttaaagcatt tttatagtat atcttttcta tatcatcttc
    18841 actaaaccct tcttttttta gaggttcata cagtttaccc atctgagata tatcttcaat
    18901 ctcaactttt gaatctattc catcaaaatc tgaccctaga gatacaacat ctattcctcc
    18961 aacatttaca atatgtttta tgtgtctaac catatcttca agtttacttt cacttttatc
    19021 acttaaaaat aagtggaaga agtttatacc tgtaactcca cctttatttg ctaatacttt
    19081 aatcatatca tcagttaaat ttctagaatg attcatcatt gctcttgaat tagaatgtgt
    19141 agcaattatt ggtttagaag aaatctttgc aatttcataa aatccaccat ctgaaatatg
    19201 agaaacatca accaacattc ccaattcatt catcttatgt accacttctc ttccaaaatc
    19261 agtaagccct ttttctctat attctacttt attatgagga aagctcaatt cattcacatg
    19321 attccaactt attgtcatca ttctaacccc taagtcgtaa aatttcttta aattttctaa
    19381 tttaccttca agtactgcac cttcctcaat agataaaaga gctgtaagtt taccttctga
    19441 ttggtttttc ataatttctt catagttagt agctaaagct atttcatcac tatttttttt
    19501 catttcctca tgaaatttat ttgccatact catgcaataa tcaaaaggat gttttacttc
    19561 ttcagtatca acaaaaagag caaatgtttg agctaatgaa tccccctttt tcaatctgtc
    19621 tatatcaaca gaatatttat tagacttaag ttcacttgtt tctacatttt ccattaattt
    19681 tgctatagta tcacaatgta aatcaagaaa tttcatattt ataccccctg tatattttat
    19741 atttatatct aaaaatagaa atagttgtcc ttatagacaa ctattgacaa ctatttttta
    19801 atattctttt taatattact taatcttatt cttcttttaa gttaggtatt acttttttaa
    19861 cttcttcaat ttgatgccat gttttatcta tattattttc actaatatat ttttttaaat
    19921 cagttaaaga tgtaaaatct ttaactgata aattgtcatc atcatctgca aatcttaaaa
    19981 taaacatgag actcccttac tttccacaac atttcttata tttttttccg cttccacatg
    20041 ggcatgaatc atttcttcct atttttgctt ctttaactat tgttttagac ttcttataag
    20101 cctttattat ttcttttctt tggtctgctg gtagtatatc ttcccaacct tgtaaattat
    20161 atagccaatg agcctcaaca gttaccatat tgttatataa tttttcccaa tctattttta
    20221 tagaaacttc tttatcagct tctacttctt ctaaaactat atcttctttt aagctttcac
    20281 ttattccatc tatgaatccc atgaaaaatt cattagtagt tccaaatttt tcagccaatt
    20341 cacttacttt accttcaaca acatccattt tattatttaa tatttcattg tatattccag
    20401 cttcaacttt caaatattct tcccagaatt ttacttctgc ttcttgactt tcatgattat
    20461 cacttaaatt tctccattca gtatataaac tcatactttt tcctcctaag tatacctata
    20521 tttttatagt ttttatagta taaaatcttt tagttagatt ggaagtttct tctttttgat
    20581 tataccatat tctatttaaa gttaactctt atattatatc ataattaaac aattctttta
    20641 atacttttat agcatactct tggtttataa aataagggct tgtcgcaatt gttattactt
    20701 tggctttttt aattaagttt tttatcttgt aaaatttgag ttcaaatggt atatagtcca
    20761 tatcacttga aaatatatcc aaatctatat caagaacaaa ctctgattct acttctaaat
    20821 caaatccata tagactatct attattatta attctgaaaa tatgttatat tttaaagcag
    20881 gttttataaa acttcccaca tttaaaactt catttgtata tctaaataca tcttttagat
    20941 tatttacatc tacatcatag ttctcaggtt ctctagtatc cttatgttgg tctacatgca
    21001 ctaacttgca tccttttgta aactcaccct ttaacaaaga ttttgcccaa aaataaaatg
    21061 catggttatg attgtcaaac acatatatct ttttatttcc caggacatat tcgaccatat
    21121 tctccaatcc ttttgctttt atttccttat tttcatctac ttcattaaat actacctcat
    21181 tgcctaacct tacatcattt aatgtaccat ttattagttt tggaacgtat atgctcttat
    21241 tttctctttt atcatatgaa aatacattat ttcctactgg tttttctata taaaatccac
    21301 tatattcata attttccata aaactttatt tcctccattg ttatataaat atataagtta
    21361 taattttaaa aattaaaatg cacttattgt aaatcatatc atctattata gaaaacatct
    21421 tatataacta aatataatat cttctctcaa tatgtattat acaataagtg caaaatatat
    21481 ttatataaaa tatactacat tcctataaca aaaccatcag cttcaattgg aaggtaacta
    21541 actcttctat tcaactccac ctttccaaaa aatactaccc aatatggtct aaaaatactt
    21601 tcaattttaa ctatttcaat ctctgggatt cctcccataa acctatgagt taatctcata
    21661 gccattttat ttgcctttgc ttttactctt cgttcgtcct tatcactata ctgcacaata
    21721 ttacaatcca cattttttac aacttcatat gtaggaaaat cttgtactat actaccagaa
    21781 cctgtagaac cattcactaa aatttttaat tcatttatat cattagaata tctcctattc
    21841 ttgaaaatgg atttcgtttt gtgaatttta agtgtgataa ttttaaactc tatataatga
    21901 agtctaactt catgagcata tttgttcttt aatattagct tactaaataa agaatttttt
    21961 ccacttttag ctatctctat agcttcttct tttgtaattc ttataggtaa cgctcttata
    22021 tccataaaat cattctcttt cttatttttt tcttgtcatt tttaaaactc tatatatata
    22081 atcaaaaaat attacaatta caactgccat aactccatat ggaattattt ttccaactat
    22141 aatatcattt ctagtcaata taccataaaa tataaatcct gttattaaaa agtacaataa
    22201 tactagaaaa ccgtctattc caagccattt tatttctaaa tcttttttta ccttagacac
    22261 taatttagac atagcagttt ctccttttat agattatttt gtgtatttaa attttcaccg
    22321 attctattac caatcatcca tgcaataaat gaaaaaggta tagctatcac aagaggctct
    22381 aactcaaagc tctgaactgg aaatatttta aaataaccaa aagtcactcc accaacaatc
    22441 attcctaaaa taccacccat agatgttctt cttccatttc caagaagccc acctactata
    22501 ggtacaaatg ccgaacccat ccaaatactt cctacaaata tacatgcatc atatacattt
    22561 tcaaatctaa atgctacgct aagccccata actgctgcta aacatacacc tattctagta
    22621 aactttacta attgtttctg agataactct ttttttgtaa ttcttccata tatatcattt
    22681 gctaatgtag ccccagctgc tagataatag ctatccaatg ttgatattat agaacctaaa
    22741 attcctatta taaatattac tcttattcct tgtggtaatg tatctaaaac taaagttata
    22801 tatccagcag ctggagccat ttctggataa gtagctctta ctataagtcc tgtcatagtt
    22861 aacaccatgt caaaacatat ccatagtgaa aaacaggtta ataaggctct tcttccaacc
    22921 ttaggagaat ctgctgctga aaatctttga taaaaagttg ggtctgaata agctccaaaa
    22981 gaatatacaa ttaacatcaa tgctttcata gctgacatac cttttgtagg gtgcataagc
    23041 tgtggtgcat ctttgagtgc ttctgtaaga ccttgaaacc ctcctatttg ttcaaattga
    23101 cctggcataa ccatagttaa accaaatacc atacaaaaaa acaataccat atctgtaatg
    23161 gcaactccca taagcccacc aagtactgta agtacaacga ctactcctac cattattaat
    23221 cctattaatt ctttatctat tttccatgct acgttaccta tagttcccat agcagttatt
    23281 tctccaagtt gtgcacaata tataaacatc aaaattgccc caattatagc aacatgcttg
    23341 ccataaaaac tttccagtaa atcaggaact gttatattgg ttcttatatg aatccttgga
    23401 ccaacccaca atgctaaagg cattctccca atatgtgcag ctattgacca aactgcccat
    23461 gcagatattc catatatagc cgcatactct acactaccta ctgtaccaac tcctccatac
    23521 catgatgcaa ccaaagttcc tactattaag gaccatggta aacttcgatt tcctaggtaa
    23581 taactttcca tatcttttac atttctagaa aaataataac ctattcctaa taatataaat
    23641 gcaaatataa ctataatccc attttcccaa aatgttaaca tatctaatct ccacattctt
    23701 atattttaat ttttaacttt aatatctaaa tatgaacaac tctacccatt tccataaaaa
    23761 taggtagagt tatataataa ttataagtat ttaagtaatc ttgttattaa gcaattttat
    23821 tgtttaaacc tttattttct cttttattta ttttatttaa agcataagta gcaaatatcc
    23881 ctataactgc acatactaca gataaaataa atatatatgt atatcctgtt tgtccatatt
    23941 tatctaacca attacctgca atagtgtaat aaaacgcatc tggagaaaat cctataaatg
    24001 aagcaaatcc aactactgct cctgtagttt ctaaagatat attagactct gaaacagatg
    24061 caaagtatat acctctaaat ccaaataaaa ttacacttag tattatcatg tttattacag
    24121 ctatccatat aaatcctgta ctcttaggaa taaccaagta agcagctgta cttactgtca
    24181 ttaatataaa tcctacaaac attactctta tagatgaacc aactttatct gttataacac
    24241 cagcaactgg agatgctccc atttttataa cataagttct tattattgat aaagcagaaa
    24301 ctagagtcat tggcattaca taaacttctg ataagtatgg acttaaataa gttaaacttg
    24361 agaatactaa ataagttgaa aatattataa gaccaattaa ccaagccttt ggtattgtaa
    24421 ctacttttaa caatgactta atacttaatc cttcatctga tgctccacta tcttttttct
    24481 cttccactaa gaaaaataga agtataccac atattataga tgctgcagaa tataaccata
    24541 ctacatattt gaatcctact gttatttcag caaatttacc aaagaaataa agacctataa
    24601 aagaaattaa tgtacctgat acacctgata acccttcata aaaaccaaat attcttcctt
    24661 gctctgactc acttccttgc attctaacaa ccttaacaga ggctgagaag aaggttaata
    24721 ttgtcgtaaa tccccataat acaaatatta aaagtatcat actatatgat gggaatgtag
    24781 agaaataaaa tccaagtatc cctgaagcaa ttaaagaaaa agttattaat tttctaactg
    24841 agaatctatc tgcaaaccat cctcctataa aataagatac cattcctact aatccatata
    24901 aactcaataa gttccctatt tgtgtatgac ttaatgctaa agcttgttgc attgggtcat
    24961 aaaatgatga tttcatatat ggtaaactat acatagctgt tgttccaaat gccaatatga
    25021 atattatcaa aaatctttta acattatttt tgctttccat tgattctctc tccttaaaat
    25081 tttattgttt ataatctcat tcatataatt ttattttaat ttttctattg cttgtttaaa
    25141 atcatttatt atatcatttg aagattctaa tcctacagct attcttatta gattttcccc
    25201 attcatttga actaatgtat gctcttcccc taagcttgtt gctattgttg caagctctaa
    25261 agagttcatt aatcttctac tagcttggtc tcttgtcaat ccactgatac catccttaag
    25321 tttaaatgaa actacgcctc cacctaaacc attcatttga gttttacaaa gctcatattg
    25381 aggatgtgat tttaatgctg gatactttac caattccaca ttctcttctt cctctaaaaa
    25441 ctctgctact gctaatccat tttcacaatg ctttttcatc ctcatatcta acgttttcat
    25501 accccttaaa atcatccatg cactcattgg tggtaaagat gctcctgtaa aacaacataa
    25561 acttggccat ctaactaaat ctattaattc ttttgaacct gctactgctc cccctaaagt
    25621 atctccatgt ccatttataa acttagtaag actatgtatt actaaatctg cacctaaatc
    25681 tattggtctt tgtacaattg gtgtcccaaa agtactatca actatagcta agcttttatt
    25741 ttcatgagca atttctgcta ttgtttttat atctactaag tctaatgatg gatttaaagg
    25801 actttctata taaactattt tagtatttgg tctcattgct tcttttacat tttgtggatt
    25861 tactgcatct acaaatgtaa cttcaactcc aaatttagat aacaactcag acatgaataa
    25921 tttagtgtgt gaaaatattc catctgaact aactatatgg tcaccacttt ttaccaatgc
    25981 taatatagta gatgttattg cccccatgcc tgatgtacta gatatggcac tttctgcatt
    26041 ttcaagcata gccaacttag cttctaactc tgtaagagta ggattacact ctcttgtata
    26101 cacatatccc catgtcatac acaactcttc aaaatgctct actgtctttg cagcaaatgt
    26161 tgctgtttga catattggtg tagccaatgc acctgttgtt gggtctgcac tatgtcccca
    26221 atgtattatt tttgtctcta aatcttgatt aatattgtta ctcattttga tttcctccca
    26281 ttttgactta tttatacaac gttttccctg ccaaatttca tcaatttagc ttagtcttaa
    26341 tacatttttt caagtatact tttttatttg ttataatttc taaaaatatg acacttattt
    26401 tgttttttta cagttctaat tataaactct atagtaacta tagagtcaat atgtttttga
    26461 tatttttttt acaatgttta aaatagtcaa tacaaaaata ggtatatctt agaataacta
    26521 aagtcatcct aagatatacc tatttttata tcacaatata aataattaca tataagcatt
    26581 agttttttta tcataaatag atttttcaag atttatcatt ttttcataaa tatattatag
    26641 ttaaatattt taatattacc taacatctac aataaaatat actcttaaaa taaaattata
    26701 taattaaaaa tactctataa aagctaaaaa gtttttctat tcgctacatc taaaaataat
    26761 tagatagagg tataaatgta tttagtttat ttatacctct agtcttttat aaattatatc
    26821 tctactaaat tgatttttta gcacgaataa gtgtgctctt ggagatacca gtcatcttca
    26881 caacctctct atatggctta gaatttaata accccaatgc atattgtatc tgttgattgc
    26941 tgtatttctt tggcctacca tctttaaagt cctttttctt tttagcaaat tcttttccta
    27001 attgagtacg ttctattatc atatttctct caagttctgc aactgcaagt aatgtagtca
    27061 aaaagaattt acccattgaa gtattttcta aaaggcctat atttaaaata tgaacactta
    27121 cttctttagc aaataaacct tctattagtt gtattccttc tgatgtactt ctagctagtc
    27181 tatccagctt agttgcaaca agtaagtctc ccttttttag agatgatata attttttcaa
    27241 atttaggcct gtttgtagta gtgcctgtat aagtttcctt tattatttta gcatcattat
    27301 atttattcaa aatttcttgc tgttgctgat acaaactgtt gttgtctgtt tgattttttg
    27361 tacttacacg gcaatatcca tatataataa tcatcacttc ctaacataaa gattttgtac
    27421 agaagttacc cagaagcttg tttttactta tctttatcca ttttactaaa acgatttttg
    27481 ggtgtcaata ctgttaactt ttgacaccat aataattcct ttataatttg tttgactatg
    27541 tcaaaaatta ccaatataat gaaattaatg agtttataca aattggagga aataatatga
    27601 atagtctgtt aaaagaagca atagatgaat taaaaaacat ccattgcggg gaagtttttt
    27661 tattaaaaga attgttcaaa ggctatgaat ggaacaggtt ggagatgaat atcagattga
    27721 aattaggaaa actttttttg tatgaagtgg taactaatcc caatttaaat gtagaggtca
    27781 tcaataaaaa ttctgcttat cagcaaatgt ataaaaaaaa atagtgactt aaatgtatat
    27841 aatagtataa tcttcttatt gattcaaaag ttaaaacttt taatacaaaa caatcattct
    27901 attttaacat ttatcaatat gtctaaattt atataattaa cacatacgaa tagtattgtt
    27961 taaatcacat tatttaagat agataaatct taaccctact ccagcataaa tactaaaata
    28021 gggcttagaa ttatctatat ttttatccca taaaatgctt agctcttcct cttgattgaa
    28081 catatatatc tggggaaata caatagaagc gtaaatttat aaaatttcca aagtcagaaa
    28141 acttcagttc aaacttaggt ataccaaaac tttcaggtgc tatttcgtca acgccaaaat
    28201 agatgataat tccattatct gtaagataaa atgcttggtc ttcaggtata actaactcaa
    28261 cattagaata atacatttct ctattttcat ttatttgaga tttaatgtaa tcagtaatta
    28321 tattaatata atcaactcca tctctaaaaa tgtcattaag taaaagttct tttccagttg
    28381 tcaaatctat attgtaatta ttcaatgtat tgtatttagg cccatagtta ttagcaaacc
    28441 ccataaggtt taatatcata ctcagtacat aatttttatt aaaagtaact gcgtaactgg
    28501 tagataccca atagctttgt tttggaagtg aattttgaga agctgcgtta ttatattctc
    28561 gttcttcttc ctgtaagcca tttttaaaat tatttacagt cattcttaca gcattgttta
    28621 tgttatttaa aataactggg tttacatacg tggattgatt tctaatattg tatataacag
    28681 gatactttac atcatatgaa aaatacttac ttctttcaaa atcttcatta aattgtacat
    28741 ataatatatt atatgtacag gtatttatag attgataatt ttccattaaa atcctccccc
    28801 ttttataaaa aaatcagata caatataata tgcttaaaac agtaataatg ttaatggtaa
    28861 atgtaaagaa tagaaagtat ctaaaacagc aaaaaatgac taattttgtg gtataataca
    28921 taggattgag agttatttta taggaggaaa aatatgttat tattagcaga taaatggaaa
    28981 gattatgaac ttatagatat gggaaatgga gaaaaattag aacgttgggg aagttatgta
    29041 cttagaagac cagacccaca ggttgtatgg ccaatggaat ctgaatgggc tttatggaaa
    29101 aaccctcatg gacattatca cagaagcaat aaaggtggag gtcaatggga acacaaaaaa
    29161 agatatccag aacaatggac tataaattat aaaaacttaa agtttcacat taaaccaact
    29221 ggctttaaac atactggatt attcccagag caagcagcta actgggattg gatgatagag
    29281 aaaataaaga aagctaacag acctataaaa gtgttgaatc tttttgcata tacaggtgga
    29341 gctactgtag catgtgcatc tgctggtgca gaagtatgtc atgttgatgc tgcaaaaggt
    29401 atggttaatt gggcaaaaga aaatatagaa ttatcaggtc ttaaaaatca aacagtaaga
    29461 tttatagtgg atgatgttgt aaagtttgtt gaaagagaaa taagaagagg taaaaaatat
    29521 gatgcaataa taatggaccc tccttcttat ggaagaggtc ctaaaggaga agtatggcaa
    29581 atagaagaaa agttatacag ttttgtagat ttatgtatgg gagttttatc agataaccct
    29641 ttatttttct taattaattc ttatacaaca ggattttctc caatagttct tgaaaatgta
    29701 ttgtctacaa caataggtaa aaagacaaat acaggagata tatacggtgg ggaattagga
    29761 ataccagctt ccagagatgg taagatactt ccttgtggta tatttggaag atgggagaaa
    29821 aaataatgat aaaagtcata tatgaagata accatttatt agttgtagaa aaaccagtta
    29881 acatattgtc tcaaggagat aatactaatg ataaagatat ggtaaatctt ttaaaagact
    29941 atattaaaaa aaagtataac aaacctggaa atgtatatat tggtttaata catagattgg
    30001 atagaccagt aggtggagtt atggtgtttg caaaaacatc aaaagcagca tctagattgt
    30061 ctgagcaggt aagaagtaag actttcaaaa aaacttatct agctgttgtt catggcaaga
    30121 taaaatctaa ctcagatata ctaaaagatt ttttatataa aaataaaaaa actaatatgg
    30181 taactgtagt gaaaaaaagc cataaagatg ccaaagaagc tgaacttaac tataatgtat
    30241 tagattttaa agaaaatcta agtttagttc aagttaattt aaagactggt cgttctcatc
    30301 aaataagagt tcaatttgca agcagaaaac atccattata cggagaccaa agatatggca
    30361 aagaaataaa taagattgga caacaaatat ctctttggtc caataagata gaaatttgcc
    30421 atcctactac aaaagaaaaa atggaattta catgtcatcc acctgacaaa tatccatgga
    30481 atatatttta aatctcaaat gaggtgatta tatgagtaaa aaattttcaa taggagaaat
    30541 gtctaaactt cataaaataa gcatacagac actaaggtac tatgaccaaa tagatttatt
    30601 taaacctgtt catgttgacc aagaaagcaa ttacagatat tactcaattg accaattttc
    30661 acaattagat attataaaat atttaaaata tttaggtatg cctttaaaag aaattaagtc
    30721 aaagcttgat gaaaataatg aagaaattct aaaattacta acaaataaaa ttaatatagt
    30781 ggatgaaaaa attaaagaat tagagcttat aaagaaggta ctaagcacaa agaaagaaac
    30841 tatggaaaaa ggtatgaaag tagatgaagt tggtaaaata gtgagaaaac atattccacg
    30901 aagaagtatc ttatctatag agtatgaaga aggattaagt agtgaagaaa ctatggaact
    30961 ttccagaaga caaatagcaa atatattaga agataatata tctgtgttct atggtggagt
    31021 aagtggactt gtatctatag ataaattttt aaaagaagat aaagttattt atgacaatag
    31081 ttttgttact gttgaaagag acttatttga tataaaatca caaaaacata taacagaaat
    31141 ttcatctggt gattttattt gtatgacata taaagggcct tacaaagaaa attataccgc
    31201 ttttaagaaa ctggttgatt atgttgaata taaaagaata cctattgaaa attatatata
    31261 tgaaatacct ataatagacc ctctgtctac aaacaacgaa gaggaacttc taacagaaat
    31321 tcaaatacaa gttaaaaaga gagaaattga cttaagatgt ctagtgtagt aatctagttt
    31381 acttacacat cttaatatag ttgtctatta tcaacatatg atttttctag atagtgattc
    31441 ctaacagtga gattttcaaa aaataggagg aaatatggct aatgctaatt ccctcctatt
    31501 tttaattaat ccatatcatt ttaattaagc catgtcatca cgaagtgcat caataatatt
    31561 ttcttttttt atcttattaa tagtatacaa cattgtaata aatactataa ataaaacact
    31621 aaatatacta attccaatac taaaccatgg aatcacaaaa tcaatatcat ctgtaaccat
    31681 tactttatga attagccatg aagaaattac tgctatagga agtccaaaga acaatgctct
    31741 catgccataa aaggcacact caaaattcat catcttttgg aaatcatgct cagacattcc
    31801 tacagagcga agcatggcaa gttctctacg acgtaactta atattagttg aaattgtatt
    31861 aaacacattg gcaatcgaaa tcagagaaat cataattatg aaagtatagg caaaaacatt
    31921 ggcaataaaa atgtaattac ggttctcatc catcattcca tacatattgt acagattgta
    31981 ctcattcgta actccctcac tctcaataat ttgctccatt tctgcaactg actgagtagg
    32041 atttttcgat tgaaagctta aactttcaat tcctgtagaa acaccaggaa tctcaaactt
    32101 atctttggct gaataaggag ccaatactct gaagcttgct ttcttcacat ctgaacttct
    32161 gattcctgga ggagaatcag gtggcactac tttgacaaat gaaatattca cattttgtct
    32221 ctcaggtatc tccaacttgc cattagtttc aggagtgagg ctaacattca tagaagaact
    32281 gttgaaaata tcaataatat tattatcctt tgttaccata gaaacagcaa tcagttttgc
    32341 attttgtcca gtatattctt ctgctggtaa tccgagactt ttaataatat tcagataagt
    32401 tctatcatca agaaattgca tatctactgg taattcaagc atttcactca tagaatgtga
    32461 accctcatag ttcaaataat cgtctgtaag ctcacttact ttggcaacac atgaataggt
    32521 cataagtttc tgatacaaac cttcgtaaac accatttgca gtttttagct tatcgtaaag
    32581 cttcaacatt tcgttgtctt tcataccttg tgtagaaaaa ataatatcct ggtcagtaac
    32641 ctctgacctc tgatttgaca tctgttttag gtctgctact aaagcacttg tagatataaa
    32701 tagaactatg cttaaaacaa gcgacaatac aatacttcga taacgtttct tgtttctttt
    32761 aaagtttttc atagcaagaa ctccctcaag accataaact cgctgtgtta gttttgatgt
    32821 cttcacagat ttagattcaa tttttacctc atttgtctga cgaatacact ccatcactgg
    32881 tgtgttggca gcctttcttg ctggtatgta agcagaaatt aagattgtaa ccatacttac
    32941 cactgctgca acgatgattg cagggataga caatgttaat gtcaacggaa cgtttacata
    33001 gagaatattt gcaaagtttt tagctatgac agaaatcaca agtccaatac tgactatacc
    33061 gattgcaaca ccaattggta taccaatcgc accaatgcaa attccttcaa atagcaccga
    33121 atttcgtaac tgttttgctg tagctccaac agatgaaagt attccaaatt gatgtgttcg
    33181 ctcgttcaat gacatgttaa atgaattata tattagaaaa attgagccta gcatgattat
    33241 ggcaagcaca atgccaccaa ccgagtacaa aaacatattg aacaaattgt tatcagaaac
    33301 acctatgaag cgcaacacat tgtcattgaa aatggaagca tggtctttag tgctacttat
    33361 gtaagagcga atctgatgtg gatttttcaa tgtgacaaaa aggctaagac tgtcagtctt
    33421 gacttgttta tcttctttgg ttatcaaggt atatcctggt gcagaagatt cttcaaatac
    33481 aggctttcta cagataccca caactgtata agttttctta tcctgtggca caaaaacttc
    33541 actcccagca gtgtatgggt cactctgacc aagattttca ttttcactca ttcgtttccc
    33601 tacatccaaa gaaatagtat cacctactgc aaatttgaca ccaccatctg tcgcaacttt
    33661 acttgaaaca agaatctctc tactattttc aggtaatctt ccagaaatta aagttatagg
    33721 caaagtatca aaagcttctt tgctaaatcc agcaataaaa agatatggtt tatctggatt
    33781 tctgttacct tcaagtgttg catagccaat attttcaaat gttgcagtat ttgcaacttc
    33841 atcattgact ctttgttcct gtacaaaaga agaatctaca tccaaaaact ggacatgcca
    33901 atcaccatat tttataatag ctccatttgt catatagttc atcaaagaaa ctccaaaggt
    33961 agtaactgct gtaatcatag cagctgacag aacaactcct ataattgtta caagagttcg
    34021 agtgcgattc tttttcaatc cttgcagagc aactttgtta aaaatgttca ttaacgattc
    34081 accttcctct catcacgcac tacttttcca tctgaaatac agataattct atctgcctgt
    34141 aaagcaatgt tttcatcatg tgtgacaata atgagagttt ggtgatattt actatggctc
    34201 tctttcagta actggataat ctcttgtcca tttttactgt ctaagctacc tgtaggctca
    34261 tcagcaagca taactgctgg tgcattcatc aatgcacgac caatggcaac acgttgctgt
    34321 tggccaccag aaagttgatt tggtaaatgt gtttttcgtt cttttaaacc aagtaattct
    34381 aacaagtcat ttaaccgttc ttgattgacc ttacgcttat ccataagtat aggcaatgtg
    34441 atattttcca ctacatttaa tgtgggtatc agattgtgaa actgataaat caatccaact
    34501 tgtcgcctac gaaaaatagc aagtttttca ttactttggc catatacatc ttgcccctcc
    34561 agatatatct ttccacttgt tggtacgtct actcctgcaa tggaatgtag tagtgtagat
    34621 ttaccagaac cagaagaacc aatgattgca gtaaactccc ctttttcaat agtaagcgag
    34681 acatggtcaa gtgcagtaac cttattttca ttcttaccat aaaccttaca taaatcttca
    34741 atctttaaaa acgccattat ataagtctcc tttcatattt tataaatata tcataggact
    34801 ttcaacttac atttaagtga catttaaatg agagattcgt cactttggaa aacgaataac
    34861 aaatattgca ccaccttgtg ggtgattttg tgcagtaatt gttcctcctt gtctcataat
    34921 aatcgtctta caaagtgcca atcctattcc ataaccggct gtgcttgaat ttttcccacg
    34981 ataaaatctg ttaaatagac atggtaaatc ttctttttta aagcctgctc cgttgtcatg
    35041 aatagtaagt tgagtaaaca aaaagttgtc ttcacaaatg atatcaattt ttccattatc
    35101 acccacactc tccatgcagt ttttaaatat attttgaatt gcttctgaaa gccaatttaa
    35161 atcacccaaa atccttactc cttttggtat atcaatatgt aaagtgatat tgtgcagttc
    35221 catgggaatc aaaagttgat gaagtgcaga actaatcaaa ttattcacat caatttgttc
    35281 tttttgaaag actacaatac ctgcatctaa acgtgataat tttaaaaggg aagtaatcag
    35341 ccagtccatt cgtataagta attcctctgt ttctcgtatc aactcttttc tctcatcttc
    35401 atcagaagta ttctctaaca gtgacaaaat aagagttact gatgtaagag gagtgcgaag
    35461 ctggtgggct atgtcagcta gtgagtcagc aaggtgttct ttttcttttt tcagtgcgta
    35521 attttgctct ctaatacgta acgtcatttt agttatttca ctttgtagaa tagaaagttc
    35581 accctcatct gattcagcaa tatacagatg gttagcatta tgaagcacaa gattaatttg
    35641 ttctgaaatc tgtgcaatac ttttgtatcg atttttagta aacacaaaaa atgctattcc
    35701 aaaggtagtg gcagaaacaa tagaaagtat tccagtcata atactgattg caaatcccag
    35761 ggctacagtg agaatagtaa ttaataagaa taaaatagca aattttcgaa actctctatt
    35821 tcgaaacata acttcccccc aatctatacc ctgtcccacg aacagtcaaa attatttgtg
    35881 gacttgaagg gtcatcttct atcttctctc gcaagcgttt gatgtacaca gtcaatgtgt
    35941 tgtcattaac aaactcacct gccgcatccc acaattcatc aagtaatctt accctcgtaa
    36001 taatactttt aggattgcta ataaacacca ataacaaacg gtattctaaa gctgaaagaa
    36061 aaacctcatt gccattcttt ttcacaacac cggttgccgt atctacataa agcccacaaa
    36121 tttcaaaagt tgacccaaaa cgcccacttt ttcgtagtgc tgttctaatt cgcgcaatca
    36181 gttcacgtgg acgaaaaggt ttagtaatat aatcatctgc acccatattc agtccagtaa
    36241 caacacttga ttcatcaccg gaagctgtca aaaagataac aggaatgtct tgcttttctt
    36301 tgatttctgt gcaaatcgta aacccatttc catcaggtaa agaaatatca ataagtgcca
    36361 aatcaaattt attcttagca atctctacaa gtgcttcact tcttgtggat acatgggtta
    36421 ctataaatcc ttctgaatta agcaaaagaa taagattttt ggcaattgct ttatcatcct
    36481 caactaaaaa tatccgtttc atttactttc atcctccttt attttattag ttttatatat
    36541 tccagcagag caatagtaca ctactatttt acttatcatt acacaatttt cagtagtttc
    36601 ttttgttctt atataatcca tgcataatac ctccattgat ttattatatt tcttataaca
    36661 taaatacaca agataagaca aaaaacataa tgtaagtgca agttttttaa ctcactaact
    36721 tataaacata cactcctact aacactactt tcttgatgac acatcttaca agcttatatt
    36781 catacatcaa tgaaaaatta aatttatcca tttattaaaa taaaaggcaa tagcttctat
    36841 agctaatgcc ctttatctta aaatgattta aatactatac aatttttata aatggctata
    36901 aataaactcg tatagaatat aaaatttatc tacagtcttt tgttatacaa aatttataac
    36961 ctcttataga acaaatttac cactttgcaa acttatcact tttactcagc tatattataa
    37021 acttttaatt tatctttgtt taaatgtttc ttcttaactt ctactaaagc ttttagcgta
    37081 tccaaagatg tcattatttc agtagaaaat tcaatggctg ttcttctcat cttaaatcca
    37141 tctcttgtag agtcatttcc tttagttggt gtatttatta ctatatcaac ttgtttattt
    37201 cttatagcat ctaatatgtt tggtctaggt tcttcaattc tatttactat atcagcctct
    37261 attccatttt ctcttaaact cttcgctgtt ccttctgtag caacaaaagt atatcctaat
    37321 tctttcatat cttttgctat ctctataaac tcatctttat catgattatt tacagtagca
    37381 agtactactc ctctttcatc agacatatgt ctacctgctg ccaagaaccc tttatataag
    37441 gcttcttcta aattttctcc tacacctagg acttctcctg tagacttcat ttcaggtcct
    37501 agacttactt caactttaga taatttagac attgagaata ctggtacttt tactgatact
    37561 aattttggct ctttgtaaac tcctgtacca tatcctaaat cctttaattt agctcctaac
    37621 atacacttag tagccaagtc aactattggt actccactta ctttacttat atatggaact
    37681 gttctactag ctctagggtt aacctcaatt atatataatt cattttgaaa ttctataaac
    37741 tgtatattaa ccataccaag cacattaagg tctaaagcta tttttttagt gtattctact
    37801 atcttttctt ttatttcatc agatatattt tgacttggat acattgttgt actatctcca
    37861 gagtgaactc cagctctttc taaatgctcc attataccag gtatcaatat atcttcttta
    37921 tcacatatag catctacttc tatttctcta ccagttagat atttatctat aagtactgga
    37981 tttttatggt ctctatcaaa tgcatcatcc aaatactgaa ttagtttttc ttcgttataa
    38041 gttatttcca taccttgacc accaagtaca taagatggtc taactaatac tggataacct
    38101 aactccttag caatttcaac accttcatta gttgtccata caccttttcc ttttggtcta
    38161 tttatatcta gtctctctag taaatcatca aatttttctc tatcttctgc tgcatctata
    38221 tctctaaagt ctgttcccag tataggtata tttttttcat gtaaaaactt agccaatttt
    38281 atagctgttt gtccaccaaa ctgtagtatt acaccttctg gtttttcttt ttctattata
    38341 tttaaaactt cttcttctgt taatggttca aaatacaact tatcagatgt atcaaaatcc
    38401 gtacttacag tttcagggtt attgtttaca attatagttt ctattcccat ttttcttaaa
    38461 gattttacac agtgaactga acaatagtca aattctattc cttgacctat tcttattgga
    38521 ccagaaccca atactacaac ttttctctta tcactaacta ctacctcatc atattgttca
    38581 taagttgaat agtaataagg tgatagagct tcaaattctc ctccacatgt atcaaccatt
    38641 ttataaactg gttgtatatt atataaactt ctcaattcat ataatttttc tgggcttatc
    38701 ttcattaagt cagatatacc tttatcagaa aatccctttt tctttaattc atgtaaatat
    38761 tctttatcta agtcttctat ttttagaact tttaactttt cttcttgttc tactatccat
    38821 ttaaatttat ttataaacca cttatcaaca ccagttattt ctgatatcat ttctacttta
    38881 tagcctcttc ttatcatttc agctaagtca aatagtctct catcatcagg tactactact
    38941 ctagccttta attcttctaa tgttctacct tctgatggag catgaaccaa tgaatatttt
    39001 cctgtttcaa gtgatcttat acctttaaga atagcagcct caaagttact tcctatactc
    39061 atgatttcac cagttgccat catcttagtt cctaattttc tattggcatg tctaaattta
    39121 tcaaatggcc actttggtat ttttactacc acataatcta atgttggctc aaaacaagca
    39181 taagtttttt tagttactgc attttctatc tcatccaaag tataacctaa agctatctta
    39241 gctgctactt ttgctattgg gtatcctgta gcttttgatg ctagggctga tgacctacta
    39301 actcttgggt ttatttctat tatcgcatat tctaaactat gtgggtttaa agctatttga
    39361 acgttacatc caccttgaac ctctattgca tttattatgt ctattgatgc ctttctaagt
    39421 agttgatact cttcatcact tagagtttga cttggagcaa caactatact gtctcccgta
    39481 tgaactccaa ctgggtctac attttccata ttacatacag ttatacagtt tccatttcca
    39541 tctctcatta cttcatattc tatttctttc caacctttta tactcttttc aagaagaact
    39601 tgtccaacag gacttagctg tagtccatgt gaaagtattt ctcttaattc ttcctcagta
    39661 tcagctatac ctccaccagt tccacctagc gtatatgctg gtcttacaac tactggatat
    39721 cctatcttat tggcaaactc aagacctgct tctaaatttg taactatatc actaactatt
    39781 actggttgat ttatttcttt cattacttct ctaaataaat ctctatcttc gccctttttt
    39841 atagattcta tagatgttcc tataactttt acattatatt tatctaatat accagcatca
    39901 tgtaattcta cagctaagtt taatcctgtc tgaccaccca tacctgcaag taaactgtct
    39961 ggtctttctt tttctattat tttttctata aattctattg ttaatggttc tatatatatt
    40021 ttatctgcaa tttctttatc agtcattata gtagctgggt tactatttac taatacaact
    40081 tcaattccct cttcttttaa tgcttggcag gcctgtgttc ctgaatagtc aaactctgca
    40141 gcttgaccta ttattattgg ccctgaccct aatactaaag ttttctttat actatctaat
    40201 ttaggcataa ctctttctcc ttatctaaac aattttcaca gttcttatct tcatcatcta
    40261 tacaatcaac tttaattact ttattttatc ttaagttcaa atttactcat caatacatct
    40321 atttaataca tctatgttaa ttaaaccaaa ctttttgatt taagttattc ttgtttttta
    40381 gttagctatt ctaatgttgt ttattttact aattctaaaa atttatcaaa aatatagtca
    40441 ttatcttttg gtcctgggca agcttcagga tggtattgaa cactgtatac atctagcttc
    40501 tcatgtcgca ttccttcaac agtattatca tttaaattta tatgtgtcac tttcatttgt
    40561 tcaggaactt ctgaaacata ataaccatga ttttgagaag ttataaatac ttttccttct
    40621 tctaaatctt taactggatg atttccacct ctatgcccaa atttaagttt agcagttttt
    40681 cctccaagag ctaaagctaa aagttgatgt cctaagcata ttccaactat aggtttttta
    40741 ccaataagag cttttatatt ttctataaca tcttctaagt cttctgggtc tccaggtcca
    40801 tttgacaaga atattaagtc agggtttata ctcaatacat cttctggaga tgttgtagct
    40861 ggaaatattg ttatatcaca tccacgagct ataaatgatc ttaatatatt tctttttact
    40921 ccaaaatcca taacagccac ttttggacca tttcctttta tatgctctac ttcttttctt
    40981 gtaacagttc taactgcttc tgtatttgaa aacttatcca atttatgttt aacatcttct
    41041 aatttactgt cttctaatgt aattatacct ttcattgttc cattatttct aagtatctta
    41101 gtcaaagctc ttgtatctat tccttcaagt ccgataactt tattttgctt taaatattgg
    41161 tctatatcaa tttcacatct aaaattactt ggagaatcac ttttctccct tactatgaat
    41221 ccctttactt gaactttttc agactctgct gatgataaat ttataccata atttcctaca
    41281 agaggataag tcatagtaac tatttgacca tagtaagaag ggtctgttaa aacttcacca
    41341 tatcctatca tcgaagtatt aaatactact tctcctacac tttcttctaa atatccaaat
    41401 gcctttccta taaaaactgt tccatcttct aatattaacc ttgctttcat actacagtac
    41461 ctcctataat tctctttaga caaactaaag aacattaact aaatataagc ttttaaatat
    41521 ctatatctat ttttcacttt tacattctag ttttatactt atttacattt attttttatc
    41581 tttttctatt atactacaaa tttcttaaaa aaaagaagtt atctcaaaag taaaaactcc
    41641 tagaaaaaat aacctctttt ttttatttaa taattaaatt cttaaactat acaatcaact
    41701 taatttctat tcagctatat tgtaaacttt taactcatct ttatttaaat gtttcttctt
    41761 aacttctact aaagctttta gtgtatccaa agatgtcatt atttcagtag aaaattcaat
    41821 agctgttctt cttatcttaa atccatctct tgtagagtca tttcctttag ttggtgtatt
    41881 tattactata tcaacttgtt tatttcttat agcatctaat atgtttggtc tagattcttc
    41941 tactctattt actatatcag cctctattcc attttctctt aagctattag ctgttccttc
    42001 tgtagcaaca aaagtatatc ctaattcttt catatctttt gctatctcta taaactcatc
    42061 tttatcatga ttatttacag tagcaagtac tactcctctt tcatcagaca tatgtctacc
    42121 tgctgccaag aaccctttat ataaggcttc ttctaaattt tctcctacac ctagaacttc
    42181 tcctgtagac ttcatttcag gtcctagact tacttcaact ttagataatt tagacattga
    42241 gaatactggt acttttactg atactaattt tggctctttg taaactcctg taccatatcc
    42301 taaatccttt aatttagccc ctaacataca cttagtagcc aagtcaacta ttggtactcc
    42361 acttacttta cttatatacg gaactgttct actagctcta gggttaacct caattatata
    42421 taattcattt tgaaattcta taaactgtat attaaccata ccaagcacat taaggtctaa
    42481 agctattttt ttagtgtatt ctactatctt ttcttttatt tcatcagata tattttgact
    42541 tggatacatt gttgtactat ctccagagtg aactccagct ctttctaaat gttccattat
    42601 accaggtatc aatatatctt ccttatcaca tatagcatct acttctattt ctctaccagt
    42661 tagatattta tctataagta ctggattttt atggtctcta tcaaatgcat cttgtaggta
    42721 ttgagataat ttttcttcgt tataagttat ttccatacct tgaccaccaa gtacataaga
    42781 tggtctaact aatactggat aacctaactc attagcaact tcaatacctt cgttaagtga
    42841 ccatacaccc tttccttttg gtctatttat atctagtctc tctagtaaat catcaaattt
    42901 ttctctatct tctgctgcat ctatatcatc aaaatcagtt cccaatatag gtatattttt
    42961 ttcatgtaaa aatttagcca attttatagc tgtttgtcca ccaaactgta gtattacacc
    43021 ttctggcttt tctttttcta ttatacttaa aacttcttct tctgttaatg gttcaaaata
    43081 cagcttatct gatgtatcaa agtctgtact tacagtttca gggttattgt ttatgattat
    43141 agtttctatg tccatttttc ttaaagattt tacacagtga actgaacaat agtcaaattc
    43201 tataccttga cctattctta ttggaccaga acctaatact atcacctttt tcttatcact
    43261 aacaactacc tcatcatatt gctcataagt tgaataatag taaggagata tagcatctat
    43321 ctcacctgca caagtatcaa ccattttata tgctggtttt atattgtata gacttctcaa
    43381 ttcatataat ttttctggac ttatcttcat taggtcagat atacctttat cagagaatcc
    43441 ctttttcttt aattcaagaa gatagtcttt agttaaatct tcaattttca taacttttaa
    43501 tttttcttct tgctctacta tccatttaaa tttatttata aaccatttat ctatacctgt
    43561 aatttgttct atcatttcta ctttatagcc tcttcttatc atttcagcta aatcaaatag
    43621 tctctcatca tcaggtacta caactctttt ctttaattcc tcaatgcttc tctcctcaga
    43681 tggtgtgtga actaatgaat attttcctgt ttcaagtgat cttatccctt taagaatagc
    43741 agcctcaaag ttacttccta tactcataat ttcaccagtt gccatcatct tagttcctaa
    43801 ttttctattt gcttttttaa atttatcaaa aggccatttt ggtatttttg ccaccacata
    43861 atctatagtt ggttcaaaac aagcataagt ttttttagtt actgcatttt ctatctcatc
    43921 taaagtataa cctaaagcta tttttgacga aactttagct attggatatc ctgtagcttt
    43981 tgatgctaaa gctgatgacc tactaactct tggatttatc tctataactg catattcaaa
    44041 actatgtgga ttaagtgcaa attgaacgtt acaaccacct tgaacttcta ctgcatttaa
    44101 tatatctaaa gatgcttttt taagcattgc acattctttg ttacttaaag tttgagttgg
    44161 agcaacaact atactgtctc ctgtatgaac tccgactggg tctatatttt ccatgttaca
    44221 tacaactata caatttccct ttgaatctct tattacctca tattctattt ctttccaacc
    44281 ttttatactc ttttcaatta atacttgtcc aacaggactt aattgtagac catgtgaaag
    44341 tatttctctt aattcttcct cattatcagc tataccccca ccagttccac ctagtgtgta
    44401 tgctggtctt acaactactg gataaccaat tgtaagtgca taatcaagac ctgcttgtaa
    44461 atctgtaact atatcactaa ctattacagg ctgatttatt tctttcatta cttctctaaa
    44521 tatatctcta tcttcacctt ttttgataga ttctactgaa gtacctatta tctttactcc
    44581 atatttatct agtatgcctt tttcataaag ttcaactgct aggtttagcc ctgtttgtcc
    44641 acccatacct gcaagtaaac tgtctggtct ttctttttct attatttttt ctataaattc
    44701 tattgttaat ggttctatat atattttatc tgccacttct ttatcagtca ttatagtggc
    44761 tggattacta tttattaata caacttcaat tccttcctct ttcaaagact ggcaagcttg
    44821 tgttcctgaa tagtcaaact ctgcagcttg acctattatt attggtcctg accctaatac
    44881 taaagttttc tttatactat ctaatttagg cataaatttt tctccttatc taaatggttt
    44941 ttactatctg taaataatac taaatctata ttaatgctaa gaatttatta aatatacaat
    45001 ctaaatcttc ttttgttgtg tattctggaa tgtattgaac actatatatt ggtaattcct
    45061 tatgtctcat tccttctata gtattgtcat ttaaatttat gtgagttact tctgtatttt
    45121 ctggcatttc tgaaacatta tatccatgat tttgagaagt tataaatacc tttccagttt
    45181 ctaaacattt aactgggtga ttacctcctc tatgtccaaa tacaagcttt gaagtctttc
    45241 ctcctaatgc caatgctaaa atttgatgtc ctaaacctat tcctgtcaat ggctttttgc
    45301 ctattaattt ttttatattt tcaactatat catttatatc ctctggattt cctgggccat
    45361 tagataataa gattaagtca ggatttatat ttaaaatttc ttctactgta gtcattgctg
    45421 gtactatagt tatatcacag ttacaagcct taagagactc tagtacacta tttttaacac
    45481 caaagtctat aactacaact ttagctccag taccttttat attttcaatt tcttttcttg
    45541 taacattttt tactgaatct ttgttacaat gctcttctaa tttagactga acatcttgta
    45601 atgatacatt ttctaaagtt ataattcctc tcttagttcc attgtttcta ataattttag
    45661 ttaatgctct tgtatctatt ccttcaagtc ctattacctt attttgcttt agatatgtgt
    45721 ctatatccat ttcacatcta aaattatttg ggttatcact tttttctctt acaataagtc
    45781 cttttacttg caccttattt gattcaacat cttctaaatt tattccatag tttcctataa
    45841 gtggataagt catagtaact atttgtccat aataagttgc atctgtcaat agttctccat
    45901 acccagccat tgaagtatta aatacaactt ctccaatagc ttcctttaga tatccaaatg
    45961 cttttccttc aaatactgtt ccatcttcta aaattaattt ccccttcatg taagctacct
    46021 cccaaatatt ttatctaagc attttctatt tctcttacaa tattaatagc tgattctctt
    46081 ggattttctg cttttgttat aggtcttcca acaactaaat aatgagcacc tttttttatt
    46141 gcatctgatg gagttacaac tcttttttgg tctccaacct ctgctgattt aggtcttatt
    46201 cctgggcaaa ctgttataaa atcagttcca catgcttcta ctattttatc tacttcttgc
    46261 gctgaacaaa caaccccatc tattcctgat tcttttgcca gtttagctct ctttatagct
    46321 aactcttctg ttgtcatatt acatcctatg tcctgtaaat cttggttgct taatgaagtt
    46381 aaaactgtta ctccaactac taatggcttt tctatattta atctttctgc ttcttcccta
    46441 gcaattagtg cacattgtct cattccttcc atatctgata catgtattgt cattaaccaa
    46501 acattatctc tcactgcagc tttcactgca cttttcatag tatttggtat atcatggaat
    46561 ttaaggtcta aaaatatttt tttccctttt tctttaagat aatctattgt tttacctttt
    46621 gtggcaacgt attgctctag ccctacctta aatatgtcta cactatcttc taatctgtct
    46681 attagttctt tagctttatc aaattcgtca gtatcaattg caactatcaa tttttcttta
    46741 ccatttatca tttttacatc tcctttttaa taaagcctaa ataatcataa aaaaatcctc
    46801 acaccaaaag aggcataagg attttatcta tacataactc tattcattag ctatgagata
    46861 tctttcccag tctctctgga ctagtttaaa agactttatt atatttaaat ttttatttta
    46921 tactcatcaa aaacctctag aagatttact agaggttata tactaacatt atctataacc
    46981 ctttagtctc tctggactac tttaaggaat ttagctgtaa tatttttctt tatttatata
    47041 attttatgta tctataggat aatttaaagt tttatctttt ttataattaa accactaatt
    47101 gctttttatg tcaataagtc tttgaattat tcttcatctt tatcaaacaa ttttttctca
    47161 aaatatgatt ctgcattata tattgcagca actggattat gcccagttac tctatctttg
    47221 acaactaaag ttgttatagg agcttccata tatttcatgg ctaaagtatc gtgaccaaca
    47281 cataatccaa gaattacatt aaggtcaacc ttagcttcat ttaaaaataa agcttgtcct
    47341 attggattgc acattacctc atctgaacac tttgatattg tttgagatgc ttctagtcca
    47401 attgtactct taggcattgc cccattttta catataacag aaaatacttc aaacccatga
    47461 tgttcaaata ttttttgcac tgtttgcatt tctcttttta gtccaataca aaatgctatg
    47521 ccaatctttt tataaccaca ctttttagca aactctataa tctcttttag tctagtatat
    47581 tgacaatacc cctctgcttc aactaatcca gcattataag ctatttttct attctcttct
    47641 tcttcataaa gttgctttgc tttttgttgt agttcaattt ctctagttgg acatatcttc
    47701 attaatttat ccatatttcc attagaacag cctctaagat tacattttcc acacttatac
    47761 atatccaata tccccttcca aaattttatt catcttgcga ttcttctttt tctagagtta
    47821 cattttcttc ttgggtttct tcttctggtt tcacatgctc gactacagtt ataaaatatg
    47881 attttcctat tttattcctt ataggaagtt tttcatatga aacatctatt ttatttttta
    47941 tacaagctat atcaaaatgt cttaaggcta cacctaagct aaccttattc atatcttcac
    48001 agtgtttctt aggtctcttt tcaaataaat gaagacctcg ttcatcctta tggaaatacc
    48061 acggttgaga attttttaca gatggagctc ttcttgtcaa ttctaagata tctttccact
    48121 ttctattcct atttatctta tttatcatat cttttactgg ctttctatca ggtctagcat
    48181 atctcgtcct aaacaactct tcattttttg ctggatatcc aaatgctatt attatataag
    48241 gattttctat tttgttttca tcatcttctt ctgtttttat tccagctaga taatctaaat
    48301 catcttcctc atcttcttca aaattcttat tcccaatatc tacaaattcc aagatatctt
    48361 ctctctttaa gtttccttcc aaccagcaag tagctattcc caatgttgta aggcgcaaaa
    48421 ctacaccttc tgtagcaaat cctatattct gtaaatagtc ctcacctttg tctgatgtaa
    48481 ctactatata atgaggtgcc tttactttac attccttacc catcaaaaaa tgtattatat
    48541 gacctctatc aacaacatgt gctttaatat ttaaatcttc atttaagtat tctaaatttt
    48601 tacaacttct ttttatttct tccatcaatg gcttttttat ctttttactg gaaaaatttc
    48661 ttacagattt tctataaaaa atggcttcat aaagctccat ttatgccctc ctaactgaaa
    48721 tcttctgacg tataaactct ataaatttat ataaatactt tatatttttt atagttttat
    48781 ttcctttttc ctcctgccct attttgaaat tcataagaat atctactcta gtttaatata
    48841 gcattcaaaa ggcttaaatt ctaccttatc aatgtctata cattagcata atttttttat
    48901 ttgattttct atgctaattt aaataacaac actagctatt aactttttaa ttaagctttg
    48961 ccagaaaaat tttttttcat ttataaatat ctatctcatt attatattat aagttttttt
    49021 cggtattctt gtatagcttt tcactaaaaa agtattttta tttctacttt aatattatta
    49081 tcaacaaaaa acatactgct taaataagtt tgttatcctc ataatctgtt ttaattaaaa
    49141 tatttttttt atttctcaat ctataaagta taaatatttt gaatatgacc caaaatctaa
    49201 gtaaagttat aatattttat aagttgttca atattaaaaa cataagtaat gttataatac
    49261 tttataagtt attcaatatt aaaaatatag gaggataaat atgaatttaa atagaaattt
    49321 agaaaaatac gctgaattag caataaaagt tggagttaac atacagccag gtcaaattct
    49381 acttataaag tcaccaatag aatgtgctga ttttgcaaga aatgcactta aagaagcata
    49441 taaatgtggt gctaaaaatg tttatataga gtggtctgat gaagaaagta ctcttattaa
    49501 atacttatat gctccagatg aagcatttca tgagttcccc aaatggacag cagaacaata
    49561 tgtagatata gcaaaagaag gtggggcatt tttatcagtc tatgcccaaa atccagattt
    49621 attaaaagat gttgaccctg aaagagttgc aaattttcag aaggcatctg gaaaagcttt
    49681 aaaagaatgg cgttcttaca ctctaactga taaatgtaaa tggagtatag tctctgtgcc
    49741 aacaaaagat tgggcaaaaa aagtgtttcc aaatttatca gaagataaag caatagaaaa
    49801 actgtgggat gctatattta aatgttcaag agtagatggt caggacccta taaaagcatg
    49861 ggaagaacat aatgaaaatc ttaaatctaa aatggacttt ctaaataaaa ataactttaa
    49921 aactttaaaa tataaatcct caaaaactga tttgactcta gacttgccaa agggtcatgt
    49981 ttggctaagt ggagcatcca aagaccctaa tggaataagt tttaatccga atattccaac
    50041 tgaagagatt tttggaatgc cacataagtt taaagtaaat ggaactgttt atagtacaaa
    50101 acctcttgtt tatggtggaa acataattga caatttcttc ttaacattta aagatggtaa
    50161 aataatagat ttctctgctg aaaagggact agatacacta gaaaaactta ttgaaacgga
    50221 tgaaggctct cattatttag gagaagtcgc tcttgttcca tacaactcac caatatccaa
    50281 tacggatata attttttata atactctata tgatgaaaat gcttcttgcc actttgcaat
    50341 tggttctgct tataagactt gtctggaagg tggaaataat ttaaaagatg aagaattaga
    50401 tgaacatggt gtaaatgaca gtttaacaca tgtagatttt atgattggtt ctgctgatat
    50461 ggatattatt ggtgagactt atgatggaaa gcaaatacag atttttaaaa atggtaattg
    50521 ggctttttaa catatgattt taactaaaaa gggctgatgg aagttatacc agacttccca
    50581 gcccttttta atgttgatga taaatatttt ttatattaat taatctcatt attatagatt
    50641 aattcctatt gttttaaaca taagtatcta aaacttatat ttatattata tataaaatat
    50701 cctatttagt aaaaccttta ttttaattta tatgctaaaa atttaacttt gctatttcaa
    50761 cagcagactt tgcatccttt gaataataat cagcacccat ttcttcagcg tattcttctg
    50821 tcaaaactgc acctcctaca aatacctttg catttatctc attatctctc agtgcctgaa
    50881 ttgtatcttt catactttga acagtcgtag tcataagtgc acttaatcct actaatttta
    50941 tatttctctt tttagcaact tccactactt cttcaatagg gacatcttta cctaaatcta
    51001 taacttcata tccataattt tctagcatta tttttactat atttttccca atatcatgga
    51061 tatcaccttt tactgtagca actattattt ttcctttgga tacattattg ctacttttac
    51121 ttaataaagt ttcctttatt gtatttaatg aaacttttac cgtttctgct gattgaatca
    51181 attgtggtaa aaatatatct ccactatcat atctttttcc tactttatca agagcaggaa
    51241 ttaaaatctc atctaatata taattttcat ccttctcttt taaaagattc agtgttacat
    51301 tttttgcttc atcttttaat cctctttcaa ctaaaatatc caagggcaaa tctttatcaa
    51361 tctttgtaga tgaatccgat ttttttgaat tatttatatt gctatattga tttatatagt
    51421 ttatacatcc tttatctgtg ttatttaata ttttaaaaga attgatagct tccatcatac
    51481 tatattcatt tggatttata attgctaaat caagccctgc ccctaaagct aatgttagaa
    51541 aagaagcatt aagtgctttc ctatttggaa gaccaaatga aatatttgaa acacctaata
    51601 tagtttttac tcctagtgtc tttaccattg ttatagcttt tatagtttct attgcttctt
    51661 cttgttgtgc tgaaacagtt aatgataaac agtctataaa tatatctttt ggttttatcc
    51721 cataagaaac tgctctatta actatccttt ttgctatttc aaatctttct tcagcctttt
    51781 taggtatccc cttttcatca agtgtaagtc caaccacaca tgaaccatac tttttaacta
    51841 ttggtaatat actttctaaa gactcctctt tgccatttac agagtttact attgttctac
    51901 cattataata tctaagtcct tgctctaagg cctctacatt ggaagaatca acttgtaaag
    51961 gtgtatctac tactgcttga atctctctta ttaattttgg cattagtttt ttttcatcaa
    52021 tctctggaag accaacattt actccaagta tttgtgctcc agcatttact tgttctaaac
    52081 ctaaatttat agcataatca acattttcat tttttaatgc ttcttgtaga gattttctac
    52141 ctgtgggatt caatctttct ccaacaactg taggcgattg tacttccaca aattttgatg
    52201 gtgaacagac tacacattta tcaattttct cgatacatcc ctttgtaaca gaatttatat
    52261 tctcagatat ttcttttata aaacttggat tcgtaccaca acatccacct attatagtag
    52321 caccaacttc aacgaacttt ttaacccctt caaaaaattc ttttgcatca acatcatata
    52381 tagcctctcc atcaactata tttggaagac ctgcatttgc ttgaaccata acagggactg
    52441 tagctcttga tgctattttt tcaactattg gtaaaagttg tttaggtcct aatgaacaat
    52501 taactcctat tgcatctaca cctagacctt ctatagtagc caccatacat tctggcatac
    52561 atccagtaaa acttcttcca ccttcatcaa atgtcatagt acagaaaata ggtaaatcag
    52621 agttttcttt tgctgctaat acagctactt tagcttcata taaatccatc atagtctcta
    52681 ttactattac atcaactcca gatttaacgc cttgtagtac ttgtctttta aatatttcat
    52741 atgctttatc aaaacttaaa gtacccatag gctctagcat ttctcctata ggaccaatat
    52801 ctaaagctac atatcttggt ttagtattat ctacattttc tatagctttt ctagccacca
    52861 aaacagcatt gtcaatcact tcttctacag tatacttact atctaatttt agttcattac
    52921 atccaaaagt atttgtaaga attacattcg aacctgcctc taagtatgct gtatgtattt
    52981 ctataagttt atctggattt tgaagaccaa atacttctgg attttcacct aactttaacc
    53041 ctttttgttg aagcattgtt cccattgcac catcaaatat taatatatta tttttcagat
    53101 attgtcttat ttccacaaga gttcgctcct tttctatatg tacagtttct atagttttta
    53161 caattttcac acgacttttc tttcttagat ttatttattc ttgtattaga aatacctata
    53221 atagccacta ctgactttct cggtatcata attccattag aagtaattgt tagacctatt
    53281 tgtttttgag cattcaaaac gtttaatata tctatatttt tttctattgg taaatctcca
    53341 tatcctgggc tatatcgcat agttataaat ttatcttgct ttaaaaaaac cttttctaaa
    53401 ttattttgaa caatgtcaca cacttcttct attgctgttg ttccacatga atcaattatt
    53461 agtccttttg ttaaatttga ataagagtgt cgtcttattt ctttttctat atctatccct
    53521 atagtagctg ccataattac acactcatta cattctttta aaagttcata tacatcttta
    53581 ctctctaatg ttaaatttgt tccctctaat tctacaacat tatctttatt tattttaata
    53641 ggataaatcc tagataaata tctaggattt attttatttt tagttatagc tacacattca
    53701 tcaatagtat atttaaggtc tttatctatc tcttgacctc tgtattgtaa atatcttaaa
    53761 acttcacttt gatttatgct tatatcttct cttccaatta aattattctc catatatatt
    53821 taaagaaaat cctttatcta aaacatcttt tgcatattcc tttgccccaa ataaagataa
    53881 gtctctgtag tttccacatt ctatagctgt agctgctggc attttatctg attcaagaac
    53941 tttttttaat gcttcttcta aaccaatctt aactgttttg gcatctacat ctccccatag
    54001 tattaaataa aatcctgttc tacatcccat tggagataag tctattacac catctaatgt
    54061 atctcttaaa taagtagcta aaagatgctc taaaccgtgc atagcagcag ttccaaaact
    54121 ttctacattt ggctgtaaaa atctcaagtc aaatttagtt actttatctc cctttactcc
    54181 atcaagtaca ctacactttc ttacaaaagg agcttttact tttgtatggt ctagcttaaa
    54241 actttctact ttttccatta tttaatcctc cattatactt taaaatttgt tacattaatt
    54301 ctaaaaaaaa ctcttcctat ctattattga taagaagagt atattatttc aagtcatctc
    54361 atcgttgtta gcaggaccac ctgataaaag gttggtatag gatcactgag ctaactctct
    54421 cccctaactc ttgataagta tcttattaaa ctgacttaaa ttatatatta cttttttatt
    54481 tttgtaaaca ctttattgtt ataatattta tatcacataa tcgtactttt attttataac
    54541 taagctgatt gtattattgg cagttgtact tcaaacacag tttgatattt atcgcttgaa
    54601 acagtaattt ctccatcatg taaatttaca atattttttg taatcgcaag acccaatcca
    54661 gaacctccaa catttctatt tctcgatttt tcaactctat aaaatctatc aaatatattt
    54721 ggtaaatcta acactggaat tggttcccca taatttatca cttttacaac cgccatatct
    54781 tcctttttct ctgtgactat atctacataa tgcccatctt ttccatattt cacagcatta
    54841 gttataagat tttcaaatgc tcttaccaat ttatttggat cagcttttac aattaatttt
    54901 tcttcattaa aattaattct actaaccata aattcttgtt taaaataaat ttcaaattga
    54961 gatactactt gacttaataa ctctactaaa tttatttcta ttttgtttaa ttttattgta
    55021 ttattttgca ttttagtcaa ttcaaacaaa tcatttataa gaacatttaa atttttcgat
    55081 ttttcatatg caatatcaac ataatacatc atctgaactt catctttata cttaccttct
    55141 tctatcactc ctaaataacc cattatggat gtcaaaggtg tccttaagtc atgtgacaca
    55201 tttgttatta aatctgtttt tgtctgctga gcttgtctct cttcaatagt tatatttcta
    55261 agttgatgga ctatattatt gattttatgt gctagttcat caatatcatc cttttcttta
    55321 tcctttaata tatctaagtc atactcaata tctatacttt tttctaagtt accttcatcc
    55381 attaaattta cagcactttt aatgatttct aatttttgat tttttctatt agtctttctt
    55441 ataaaatcta caatttgctt atttctcttg tacgatatca tggaataaat tgctaatgat
    55501 gtgattgtaa ataaaaatgg ataaaataaa ctctctccta taagacttct gagatctctt
    55561 aactgatata caagttgatt aattacagat atcccactat tgaaatataa tgattttatt
    55621 atatacatac ttgttaaaat aagcattgaa gttaaagcac agcttattat aatatctaat
    55681 atgggtcgaa acatatgatt taataaactt ttatttttca attttatatc caactcccca
    55741 tacagtatga attatttttt ctccatctgt atatttttca attttatctc ttattctgct
    55801 catatgaacc attacagtat tattagattg gtaatatttc tctttccata ccttttcaaa
    55861 tatttcttct gtactatata ctctgccttt attatttgct agtaaatata atatatcaaa
    55921 ttctcttgat gtcaaaatta tttcatttcc agccactgtt acactatgtt tttccttatc
    55981 tataattact actccaacct ttatgacatc tttatcttca ttattagcac cactaaaata
    56041 atatgctctt ctaatcagcg acttcactct cacagtaagt tcaagtgggt taaatggttt
    56101 tgtcaaatag tcatctgcac cagtcattat cccttgtatt ttatccatgt cttctgattt
    56161 tgcacttaac ataagtattg gtatattcaa actttctcta acttttctac aaacttctat
    56221 cccatccatt ttaggcatca ttatatccaa tatcattaaa tgaatatttt ccttttttaa
    56281 aatatctaga gcctcttttc catcttcagc tttaattaca ttatatccac tatttaaaag
    56341 atatatctct ataaggtctc ttatttcctt ttcatcatcc acaacaagaa ttgtattatt
    56401 catttttaca catcctaact taatctttgt atatagcttt attcatataa aattatacta
    56461 tattttagtt agtgtaatct ataaatttat taatttctaa atttccaata taatacaaat
    56521 tatttacaca tttatattat ttattttgta aatattcttc taaagttaat ccttttgaat
    56581 aaatttcctc ggcagcatcc ttaccaacat atctaatatg ccatggttca tatgctgtac
    56641 ctgttatatc ttctttacct tcaggatatc ttattataaa tccaaacttg tgagcatttt
    56701 ctgccaacca tttagcttct tttgtacttc caacaaacca tctttctgga tttgttaaat
    56761 caatacaaag tccagtttgg tgctcactgt aaccaggctt tgctacatat gcatctgcct
    56821 tttctcgtcc ctgagatttt acccttcttg tataagtatc cagttgagta tcataagatc
    56881 tgtatgctga agaccctaaa aactgtattc cttctgaatt agcttgctta actaaatctt
    56941 caactgcctt tgcaggttct cctgccattt gtttttcttc ctctgttgct tcttcaacaa
    57001 aaggtatgtt taccttagta atattttcag gagtatagtt tttgctaatt ccatttgtct
    57061 tgtttaccaa taatatattt gatatactat ttgagtgatt tttattttga gaatttgcct
    57121 caacatcttg ttctgaatca tgttgagcag ctactattgg atctttatct ttcttaagta
    57181 atctatcttt agatataaat acacttataa atactactaa aataatggct cctattaatt
    57241 taaatttttt cataattaaa tcttctcctt tttgttatgt ttgctataat gttaatttta
    57301 gagtataaga cttaagtatt ttataagtaa atcttaaata aatcttaaat ttaatatttt
    57361 taaaggatgg tgataaaatg tctatacaaa aacttctaag taaagctcgt aaggctatac
    57421 aagattttga tatgatacaa gaaaatgata aaattgcggt tggactttct ggtggaaaag
    57481 atagtttaac tttacttcat atacttaaaa gttatcaaag attttctcca caaaactttg
    57541 aattgatagc cataacatta aatactggag gtgttgataa ttctccacta gataaattgt
    57601 gtaaagaaat aaatgttcct ttttatgaat ttcaaactga cataaaagaa attgtatttg
    57661 atataagaca agaaaagaat ccttgtgcat tatgtgctaa tttaagaaga ggtgctttga
    57721 acgataatgc taaaaaactt ggatgtaata aagtagctct tggtcatcat aaagatgatg
    57781 caattgagac atttttaatg tctatgtttt atgagggtag agtaaattgt ttttccccaa
    57841 agacatacct agacagacag gatttaacag ttataagacc tatggtttac atagaagagt
    57901 atatgactaa gaaaatttca aaagattcta actatcctat tatcacaaat ccctgccctg
    57961 caaatggtca tactaggaga gagtacataa aaaatcttat tgctaattta aataaagaga
    58021 tgcctgattt taaaagaaat gtatttggtg ctttaaataa ttctgagaaa ttgtttatat
    58081 gggataaaga gaagataaaa aattttaaat aataattaaa atttttaaat aaaaaagcaa
    58141 gattgtatta attgtaaata atctttatat aatctacaca atcttacttt tttatttatt
    58201 atatgttatt atctttaatc tatattttaa tagttcttaa tagtatttat tttgtctaat
    58261 acattaatta taacttaaat attttatata ttaatttaat ctattaattc attcaatcta
    58321 ctggtttaat ccatttttac agtatataca tctacatatt ataaaaagca aaattatatt
    58381 ttctaaatat tttatgttag attataatat taaattctat tatctctggt atattaaaaa
    58441 atctaaatgg taactttgta cttccaagac ccgaactcac atataaatga tttccattta
    58501 tatcataaaa ccctttagta tatttccttc catatggagg agtaactaat gctcccttaa
    58561 taggtaatct tatttgacca ccatgactat gcccagatag aactaaattc attgtatctt
    58621 tactaagcat atctactaaa tctggttcat gtgctaacaa taaattaaaa cttttattat
    58681 ctatttgact ctccagatat tttatattag gatttccatt taatatttca tcaacaccta
    58741 atatattaat atatttatca tcttttaatt ttattttttt attttcattt actaaaagat
    58801 tgaagttgga atttttcata atctgcct
//
LOCUS       DAGSHT010000006        53511 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGSHT010000006 DAGSHT010000000
VERSION     DAGSHT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.007"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            281..841
                     /locus_tag="MJB72_000311"
     CDS             281..841
                     /locus_tag="MJB72_000311"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBY8261489.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITNCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            858..4244
                     /gene="mfd"
                     /locus_tag="MJB72_000312"
     CDS             858..4244
                     /gene="mfd"
                     /locus_tag="MJB72_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBY8261490.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            4275..5270
                     /locus_tag="MJB72_000313"
     CDS             4275..5270
                     /locus_tag="MJB72_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY8261491.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            5394..5933
                     /gene="spoVT"
                     /locus_tag="MJB72_000314"
     CDS             5394..5933
                     /gene="spoVT"
                     /locus_tag="MJB72_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBY8261492.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            6053..7666
                     /locus_tag="MJB72_000315"
     CDS             6053..7666
                     /locus_tag="MJB72_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY8261493.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            7773..9221
                     /gene="mazG"
                     /locus_tag="MJB72_000316"
     CDS             7773..9221
                     /gene="mazG"
                     /locus_tag="MJB72_000316"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBY8261494.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            9310..9588
                     /locus_tag="MJB72_000317"
     CDS             9310..9588
                     /locus_tag="MJB72_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBY8261495.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            9660..9902
                     /locus_tag="MJB72_000318"
     CDS             9660..9902
                     /locus_tag="MJB72_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBY8261496.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            9964..10218
                     /locus_tag="MJB72_000319"
     CDS             9964..10218
                     /locus_tag="MJB72_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBY8261497.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            10219..10752
                     /locus_tag="MJB72_000320"
     CDS             10219..10752
                     /locus_tag="MJB72_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261498.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            10758..11042
                     /locus_tag="MJB72_000321"
     CDS             10758..11042
                     /locus_tag="MJB72_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBY8261499.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            11155..12075
                     /locus_tag="MJB72_000322"
     CDS             11155..12075
                     /locus_tag="MJB72_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBY8261500.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDLIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            12165..14537
                     /gene="spoIIE"
                     /locus_tag="MJB72_000323"
     CDS             12165..14537
                     /gene="spoIIE"
                     /locus_tag="MJB72_000323"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBY8261501.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVESIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            14888..16780
                     /gene="pepF"
                     /locus_tag="MJB72_000324"
     CDS             14888..16780
                     /gene="pepF"
                     /locus_tag="MJB72_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBY8261502.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            16894..17859
                     /gene="galU"
                     /locus_tag="MJB72_000325"
     CDS             16894..17859
                     /gene="galU"
                     /locus_tag="MJB72_000325"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBY8261503.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            18359..19963
                     /gene="rho"
                     /locus_tag="MJB72_000326"
     CDS             18359..19963
                     /gene="rho"
                     /locus_tag="MJB72_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBY8261504.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            20116..20316
                     /gene="rpmE"
                     /locus_tag="MJB72_000327"
     CDS             20116..20316
                     /gene="rpmE"
                     /locus_tag="MJB72_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBY8261505.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            20461..21354
                     /locus_tag="MJB72_000328"
     CDS             20461..21354
                     /locus_tag="MJB72_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBY8261506.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            21372..22220
                     /gene="prmC"
                     /locus_tag="MJB72_000329"
     CDS             21372..22220
                     /gene="prmC"
                     /locus_tag="MJB72_000329"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBY8261507.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            22264..23328
                     /gene="prfA"
                     /locus_tag="MJB72_000330"
     CDS             22264..23328
                     /gene="prfA"
                     /locus_tag="MJB72_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBY8261508.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            23457..24137
                     /locus_tag="MJB72_000331"
     CDS             23457..24137
                     /locus_tag="MJB72_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBY8261509.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            24166..25206
                     /locus_tag="MJB72_000332"
     CDS             24166..25206
                     /locus_tag="MJB72_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBY8261510.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            25223..25672
                     /locus_tag="MJB72_000333"
     CDS             25223..25672
                     /locus_tag="MJB72_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBY8261511.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            25689..26141
                     /gene="rpiB"
                     /locus_tag="MJB72_000334"
     CDS             25689..26141
                     /gene="rpiB"
                     /locus_tag="MJB72_000334"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBY8261512.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            26177..26806
                     /gene="upp"
                     /locus_tag="MJB72_000335"
     CDS             26177..26806
                     /gene="upp"
                     /locus_tag="MJB72_000335"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBY8261513.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            27107..27922
                     /locus_tag="MJB72_000336"
     CDS             27107..27922
                     /locus_tag="MJB72_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBY8261514.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            28148..28585
                     /locus_tag="MJB72_000337"
     CDS             28148..28585
                     /locus_tag="MJB72_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBY8261515.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            28770..28997
                     /locus_tag="MJB72_000338"
     CDS             28770..28997
                     /locus_tag="MJB72_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBY8261516.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            29002..29388
                     /locus_tag="MJB72_000339"
     CDS             29002..29388
                     /locus_tag="MJB72_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBY8261517.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            29391..30095
                     /gene="atpB"
                     /locus_tag="MJB72_000340"
     CDS             29391..30095
                     /gene="atpB"
                     /locus_tag="MJB72_000340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBY8261518.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            30164..30424
                     /gene="atpE"
                     /locus_tag="MJB72_000341"
     CDS             30164..30424
                     /gene="atpE"
                     /locus_tag="MJB72_000341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBY8261519.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            30547..31044
                     /gene="atpF"
                     /locus_tag="MJB72_000342"
     CDS             30547..31044
                     /gene="atpF"
                     /locus_tag="MJB72_000342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBY8261520.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            31041..31586
                     /locus_tag="MJB72_000343"
     CDS             31041..31586
                     /locus_tag="MJB72_000343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBY8261521.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            31603..33105
                     /locus_tag="MJB72_000344"
     CDS             31603..33105
                     /locus_tag="MJB72_000344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBY8261522.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            33133..34011
                     /gene="atpG"
                     /locus_tag="MJB72_000345"
     CDS             33133..34011
                     /gene="atpG"
                     /locus_tag="MJB72_000345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBY8261523.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            34024..35418
                     /gene="atpD"
                     /locus_tag="MJB72_000346"
     CDS             34024..35418
                     /gene="atpD"
                     /locus_tag="MJB72_000346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBY8261524.1"
                     /translation="MANVGKVVQIVGAVLDVKFDGEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            35421..35681
                     /gene="atpC"
                     /locus_tag="MJB72_000347"
     CDS             35421..35681
                     /gene="atpC"
                     /locus_tag="MJB72_000347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBY8261525.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            36143..36523
                     /locus_tag="MJB72_000348"
     CDS             36143..36523
                     /locus_tag="MJB72_000348"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBY8261526.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            36538..36999
                     /locus_tag="MJB72_000349"
     CDS             36538..36999
                     /locus_tag="MJB72_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBY8261527.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            37020..37610
                     /locus_tag="MJB72_000350"
     CDS             37020..37610
                     /locus_tag="MJB72_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261528.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            37625..38782
                     /gene="alr"
                     /locus_tag="MJB72_000351"
     CDS             37625..38782
                     /gene="alr"
                     /locus_tag="MJB72_000351"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBY8261529.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            38815..39102
                     /locus_tag="MJB72_000352"
     CDS             38815..39102
                     /locus_tag="MJB72_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBY8261530.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            39071..39454
                     /locus_tag="MJB72_000353"
     CDS             39071..39454
                     /locus_tag="MJB72_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBY8261531.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            39625..40452
                     /locus_tag="MJB72_000354"
     CDS             39625..40452
                     /locus_tag="MJB72_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBY8261532.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            40445..41386
                     /locus_tag="MJB72_000355"
     CDS             40445..41386
                     /locus_tag="MJB72_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBY8261533.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            41810..42904
                     /locus_tag="MJB72_000356"
     CDS             41810..42904
                     /locus_tag="MJB72_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261534.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            43130..43546
                     /locus_tag="MJB72_000357"
     CDS             43130..43546
                     /locus_tag="MJB72_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261535.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(43728..44297)
                     /locus_tag="MJB72_000358"
     CDS             complement(43728..44297)
                     /locus_tag="MJB72_000358"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBY8261536.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            44451..45608
                     /locus_tag="MJB72_000359"
     CDS             44451..45608
                     /locus_tag="MJB72_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBY8261537.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            complement(45791..46945)
                     /gene="nagA"
                     /locus_tag="MJB72_000360"
     CDS             complement(45791..46945)
                     /gene="nagA"
                     /locus_tag="MJB72_000360"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBY8261538.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNNTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYIPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICMYKVNK"
     gene            complement(47028..47753)
                     /locus_tag="MJB72_000361"
     CDS             complement(47028..47753)
                     /locus_tag="MJB72_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8261539.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITTGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            48020..48949
                     /gene="pfkB"
                     /locus_tag="MJB72_000362"
     CDS             48020..48949
                     /gene="pfkB"
                     /locus_tag="MJB72_000362"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBY8261540.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNTCRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(49078..49995)
                     /locus_tag="MJB72_000363"
     CDS             complement(49078..49995)
                     /locus_tag="MJB72_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY8261541.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            50466..51635
                     /locus_tag="MJB72_000364"
     CDS             50466..51635
                     /locus_tag="MJB72_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY8261542.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            51684..52535
                     /locus_tag="MJB72_000365"
     CDS             51684..52535
                     /locus_tag="MJB72_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBY8261543.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            52861..>53511
                     /locus_tag="MJB72_000366"
     CDS             52861..>53511
                     /locus_tag="MJB72_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBY8261544.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIR"
BASE COUNT        21205 a         5875 c         9817 g        16614 t
ORIGIN      
        1 aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc attgggaaat
       61 aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt ttttattatt
      121 ttatacataa ctaaagtatt taaagatgta gatataagaa agcaaattcc ttttacacct
      181 tttatttcct tgtcgacaat tttaataata tttacagagt ataatatatt taatttttat
      241 tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag tagtagggtt
      301 aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg atgttataga
      361 tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag ctttgattgg
      421 tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt atatgaattt
      481 aagtggagaa acattaatag atatatataa atattataag gttgatttaa gtaatatagt
      541 agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa aaaaaggaag
      601 tggaggaact cataatggca tgaaatctat aactaattgc ctaggttcaa atgactttcc
      661 aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag actttgtact
      721 ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa aagcggcaga
      781 tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat ataatggata
      841 gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat tcaaaagagt
      901 ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt aatggacttt
      961 taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca cgacaaatga
     1021 tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta agtttttata
     1081 tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac ttggatgcaa
     1141 aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca aataaagaaa
     1201 aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca aaacaagttt
     1261 tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa aagttaactg
     1321 agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt ggtcaattta
     1381 gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca attcgtatgg
     1441 aattatttga tgatgagata gattccatac gaacttttga cgtatattca caaaaatcaa
     1501 ttgataaatt gcaacaattc tctataactc catctaggga atttatttat cccgaaaaaa
     1561 ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat gaaggtacat
     1621 ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag aactatatag
     1681 attatattta tccagaagaa aacaaaagta tatttactta tttagcagat gatgcaatag
     1741 tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata aatgagttta
     1801 gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa ggtaaattgt
     1861 tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta ttaaactcgc
     1921 tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt gaaagtagag
     1981 aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat cggctgaaat
     2041 ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat aaattaggaa
     2101 aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat attgaaatta
     2161 aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag tataaatcta
     2221 taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga gcgtctaaga
     2281 ctagcaataa aaaagttaaa aagggaaaga aaatagaatc ctttttagat ttaagtgttg
     2341 gagattatgt agtccatgaa aatagtggtg taggtagata cacaggaatt gaacaaatta
     2401 cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga gataatctat
     2461 atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa gtagaaaaag
     2521 ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa gttaaaaaag
     2581 aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa aaaatacaag
     2641 ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg tttccatatc
     2701 aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg gaatcaagta
     2761 aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag gtagctatta
     2821 gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt ccaacaacaa
     2881 tattggccca acaacattat aatacattca aagagaggtt tgaaaattat cctttgagag
     2941 tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt gaagatgcta
     3001 aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa gatataaatt
     3061 taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta aaacataaag
     3121 aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca actcctatac
     3181 caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata gaagaacctc
     3241 cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc gtaattcaag
     3301 atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac aatagagttg
     3361 aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct agagtggctg
     3421 ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga tttttaaata
     3481 aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac atatcaaatg
     3541 caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt taccaattga
     3601 gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat gaaaaagata
     3661 aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt acagagtttg
     3721 gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga aatattttag
     3781 gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt aaaatgctta
     3841 atgatgcaat taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat gttgaaatag
     3901 atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg attaaaattg
     3961 aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat atacaagaag
     4021 aacttgagga tagattctct gatataccta aaccagtcca gacacttctt acaatagctt
     4081 atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta aaagatgaaa
     4141 tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat aatatagtta
     4201 cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta aatttaagaa
     4261 gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt ttggtagtat
     4321 ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa ggtacaaaga
     4381 tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg gaacaaactt
     4441 atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag gacaagttta
     4501 aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa gctaaaaaag
     4561 ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta aaagacgcta
     4621 tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat gatgagtttt
     4681 taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac tttgctaaaa
     4741 agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa gatgaattta
     4801 aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat aataacaagc
     4861 cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct cttaaagaag
     4921 ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat acttcagcaa
     4981 gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa tttgaagatg
     5041 ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa tatggatacc
     5101 acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat gctaaagaaa
     5161 ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa ttaactaaag
     5221 aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa gacttaaaaa
     5281 aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt tgtatatttt
     5341 tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa aatatgaaag
     5401 caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca aaagagataa
     5461 gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca aaagatggag
     5521 aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg caagaatatg
     5581 ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta gattctataa
     5641 tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt gatgagttag
     5701 aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt ttaccattat
     5761 tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt agtgcatctg
     5821 gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt gcagaaagtg
     5881 aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa taattttatc
     5941 ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg aatatagcat
     6001 taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg ttatgaacaa
     6061 taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag caggtgtaat
     6121 cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg gttcagaagg
     6181 aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat tagctactgc
     6241 tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg gaaattttaa
     6301 aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg gtataatatc
     6361 attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga aaaatccagg
     6421 tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg caatgtctgc
     6481 atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt ctcaagttgc
     6541 agagcaattt tttagagttg tattaggtct tactttggct tacttgctta tgaagagtct
     6601 aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt caatagcatc
     6661 tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag cagagataga
     6721 tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc ttttaactgt
     6781 tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga tagataatgt
     6841 tattgtaata agaagactta tggaagcagg ctttacatac aaagtggcaa attccatgtt
     6901 tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca taacaactgc
     6961 aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa ataagtcaaa
     7021 agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat tgccttgtgc
     7081 gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac atgaaccatc
     7141 ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag gattaataca
     7201 gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa ttgctttatg
     7261 tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac cagatattaa
     7321 tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca tgattaactt
     7381 actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta taataaagcc
     7441 atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg ctttagttgg
     7501 attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa ttgtatatgt
     7561 agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta tgccaaaagg
     7621 tgataaatta tataaattgt taaaaaaagt taaacttata agataatata ggaatatata
     7681 tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa taatataaaa
     7741 ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt gtaggtcttg
     7801 gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata aatagcccta
     7861 aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa actatacaat
     7921 acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt tatttgaaaa
     7981 tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct gttcctggtc
     8041 atccaagagt tgcagagaag actgtaagta taatagagag attagccaat gaaaagcaca
     8101 tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac tatttggcta
     8161 tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat tcatatatag
     8221 acttggatac aagtatgata ataactcagg tatatgataa atttatagca tctaatgtaa
     8281 agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta aatggagctg
     8341 gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac agaaatgaaa
     8401 acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag atgtataata
     8461 cagtacatga cttagaaata atagttaata cacttagaag tccatctgga tgtgaatggg
     8521 ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca tatgaacttt
     8581 gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt gatgttcttt
     8641 tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat ttaaaagaag
     8701 tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt agtggtgcaa
     8761 aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa gagaaaggcg
     8821 aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct cttatgaagg
     8881 ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat gtagaagatg
     8941 tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg gtcgggaata
     9001 taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat ctagctagat
     9061 ttttagacat agactcagga gaagccttga actgtactac agaaaaattc ataaatagat
     9121 ttgactttat agaaaagagt gcaatgaact tgaataaaaa attagaagac atgacacttg
     9181 aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga aggaaatttg
     9241 tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca tttttaggag
     9301 gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag tggattaaca
     9361 aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga tgcactagta
     9421 aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga aagagctgct
     9481 agacaaggaa gaaatccaag agatccagag caagttatag atataccagc ttctaaagca
     9541 ccagttttca aagctggaaa aggattaaag gatataataa atggataatt agaaatcttg
     9601 tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag gtgataatta
     9661 tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact gttgctaaag
     9721 atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct tcttcagagg
     9781 tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag tttaagataa
     9841 atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa attttagaat
     9901 aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta ctggggggat
     9961 attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc gggagtcgag
    10021 cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg cgagatggta
    10081 attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat aattagcata
    10141 gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga gagtttcttg
    10201 aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt ttatgctact
    10261 atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct aagagggaat
    10321 tttaaattca tcaattattt tgctataata tttgatgtat tgttttggtt tctagctacg
    10381 gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata ttatcatttt
    10441 gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat tgttttatca
    10501 attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac acattacata
    10561 gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct actatttgat
    10621 attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa aagaaaatca
    10681 aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa taaaactaaa
    10741 aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt attgttatca
    10801 gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt gagtatacaa
    10861 aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa aagactgaaa
    10921 tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta gaggatatag
    10981 caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac ataaatcgtt
    11041 gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag atttaataaa
    11101 aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt cataatgaaa
    11161 gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga ttatagagat
    11221 gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta tggtgtggat
    11281 agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt aaaagaattt
    11341 tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac atctgctctt
    11401 agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac tggaatagat
    11461 gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt tttagaagga
    11521 atacaaggtg atgatactat tattgttata gatattggag gaggttcaac agaatttatt
    11581 gtaggtgatt cagaaggaat taagtttaat aaaagtgaaa atgtaggtgc tcttaggatg
    11641 acagagaggt ttttgaagca cgatcttatt gaagatgaag agtttgaagc tatgagttca
    11701 tttatatatg gtgagattga caaaacgtta aatcatataa aagacagagg attaaaaact
    11761 cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga attagagatt
    11821 tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga agaaattcta
    11881 caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct acaagaaaaa
    11941 cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga aaaattagaa
    12001 atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg tcaaaagtca
    12061 aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa aaattactcc
    12121 tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa agaagtgtag
    12181 cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc ggatatattg
    12241 attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct attatagtag
    12301 actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt gataagtaca
    12361 agatacctgt attcatatca acattattag gaatattgtt atcatctaat gattttggaa
    12421 atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa aaattaaaat
    12481 tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt ccaatctcca
    12541 tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca ggagtagaat
    12601 caataataat gtttatatct atatatgtgt tttcttatgg gataggttta atggccaatg
    12661 tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta ctgctaacct
    12721 ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg accattttat
    12781 cgaccgtatt gatattgctg acctcaatag taggtggagc taccatggga gcatctagtg
    12841 gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt tatatgggga
    12901 tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa tatttctgta
    12961 tactaggata tattctaagt tggacaataa tatacctata tacttctggt attacttcta
    13021 atatgatgca attaagagat atactattag gttgtttgat agtactagta ttgccagagc
    13081 gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat gaaatcgtat
    13141 acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt atttataaaa
    13201 cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa gtgctagacc
    13261 aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa agttgtagta
    13321 tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata tataacattc
    13381 ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa aattttagaa
    13441 aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac aaaatgtttg
    13501 tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata gcaaatcaga
    13561 taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc gacataatgc
    13621 tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat ataacagttg
    13681 ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata gaaaagaaaa
    13741 cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca gacctagtag
    13801 gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa tgcaaggcaa
    13861 cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg tcaagggatg
    13921 ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt aaatacatga
    13981 tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct tctgccacta
    14041 ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc attgatacta
    14101 taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg gatttgggaa
    14161 ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt tctacttata
    14221 taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct gtgggtatat
    14281 tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac tatgttataa
    14341 tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac aattggttaa
    14401 tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat ttaatattgg
    14461 atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt ttagttacga
    14521 aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata acttgtgtat
    14581 aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca tataaatcaa
    14641 caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag aaaaaacaaa
    14701 agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa gaaaacctgt
    14761 gaatatgttg attattatgt ggataactat taattcgata tttatttata tagtatgaat
    14821 aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat tgaaaggatg
    14881 gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt ctattttttg
    14941 catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg acagagcaat
    15001 tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag aaatagacta
    15061 tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat ggaaaaaaga
    15121 acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta aagtaacaaa
    15181 atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata taagactaaa
    15241 taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt ataagtactt
    15301 agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt gttcagattt
    15361 agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt caaataaaaa
    15421 tataaatagg aaatatggtg catatttaag agatataaga aggaataaag tacattattt
    15481 ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc ttccaagcca
    15541 agtgtatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag ctcaatatgc
    15601 gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag atgaacttat
    15661 aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag ttaaaaagaa
    15721 tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc tggaatctga
    15781 taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta atatggacag
    15841 tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg ttcattcata
    15901 tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg aaaaggcaca
    15961 aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg tagtatataa
    16021 agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa gtggtggata
    16081 ctgcttatct gtttatgaaa atcatccata tatactttta aattataata attcattagg
    16141 ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt taagttctaa
    16201 gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg catctacaac
    16261 gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg atgaaaaagc
    16321 ttattatata acacagtatt tagacttgat aaaagatact ctttatacac aaactatgta
    16381 tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg taaacgcatt
    16441 agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa gctatgaatt
    16501 agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata gtttctatgt
    16561 ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca tcctaaagaa
    16621 tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc caataaactt
    16681 gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata caatcaaaaa
    16741 atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt aatccacatg
    16801 gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat caagatagga
    16861 ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa aaaggctgta
    16921 ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca accaaaagag
    16981 atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc tgtggcctca
    17041 ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga agaccacttt
    17101 gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga attgttggag
    17161 gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa agagccaaaa
    17221 gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc gtttgcagta
    17281 atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact aacagatgca
    17341 tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga agatactaat
    17401 aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt aaaagacatg
    17461 gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg tagatatata
    17521 ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg tggagaggta
    17581 caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc atacaatttt
    17641 gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac tgttgacttt
    17701 gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca tgtatgtagt
    17761 gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat agatttaatt
    17821 gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg tgatgtatgg
    17881 tggcaaaatc aagtcagaaa aaaattaata gaataatagc tgttgggatt gtgggaatcc
    17941 tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa taagataaat
    18001 gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag tcaaaaactt
    18061 acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa atttttataa
    18121 tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga taaatttata
    18181 aaaacgtgct aagattggac ttgaaataca gtaatatagt aggtaataat gttttaaatt
    18241 ttatttattg accataatat ataaataatg atattattag acttaactgt ttaaataaaa
    18301 cttttctttt tcatgccttc aatttccaaa ataagtatgt atataaatga atatatagat
    18361 gaggaggatt tttttgattt tagatgataa agtattagaa ggattaggta gtaagacctt
    18421 agttgagcta cgagagatag ctaaagaatt aaaaataaaa tcaattacta catataagaa
    18481 aaatgagtta attgagatta taaattccaa aagtaaaaat gaggaaaaag taaacgaaga
    18541 gactaaagaa aaaaatgaag atattaaaga agacataaag aatagaaaag aaaatataga
    18601 gcgtgaaagt atagaaaaag aagttgaata taagagccct accaaatcat acaacaagaa
    18661 agaaatagat acccaaaata tttcccaaaa ccaagaacat agaaatcaaa gaaatgacta
    18721 taataagaca acgagtgtaa atgatagcaa taagtcatca aatatatcta atagaatggt
    18781 cagaaataat aataaaaact attatatgcc taaacaagtt gatgaatcta agatagtaga
    18841 tgaatttaac acatcaaaag aggatgaagt agtaggtgta cttgaaattc tgccagatgg
    18901 atttggtttt ttaagaggtt caaattattt gtctactgaa ggagatgttt acgtttcacc
    18961 atctcaaata agaagattta atatgaaaac aggagacaag attaagggaa taactagaca
    19021 tccaaaaagt ggagagaaat ttagagcact tttatatgtt caaaaaataa atgatgaaaa
    19081 ccctgacaca gctatacaaa gaaatgcgtt tgagacatta acaccaatat ttccagaaga
    19141 acgacttaca ctggaaacta atagaaatga aatagctaca agaattatag atttaatttc
    19201 acctatagga aaaggacaaa gaggattgat agtagcacct ccaaaagctg ggaaaacagt
    19261 tcttttaaaa agcgttgcaa atagcatagc taaaaatcat ccaaatgttg aacttatagt
    19321 actccttata gatgaaagac cagaggaagt tactgatatg aaagaatcta ttgaaggaga
    19381 cgttatatat tccacttttg accaagtatc aagtcatcat gtcaaggttg cagagatggt
    19441 gttaaataga gctcaaagat tagttgaaca tggtaaagat gtagtaattt tactagatag
    19501 tataactaga ttggcaaggg catataacct tacaatatct cctacaggta gaactttatc
    19561 tggaggtatt gaccctggtg cacttcatgg acctaaaaag ttctttggtg cagcaaggaa
    19621 tattagacag ggtggttctt tgacaatatt aggaacagca ttggttgaaa ctggttctag
    19681 gatggatgat gtaatatttg aggaatttaa aggtacagga aatatggagc tacatttaga
    19741 tagaaaatta gcagaaaaaa gaatattccc tgcaatagat atttacaagt ctggtacaag
    19801 aagagatgat ttattgcttg atgatgaaga aaagactgct ctttggagat taagaagaga
    19861 aatgagtaat aattcagtaa tggaaattac agataaagtc attgaactta taaagagaac
    19921 taaggacaat aaagagtttg taaaatcaat aaagaatttg taaaatcaat aatacaaatg
    19981 caaatatgga ttatataaac taatagaaaa atcacttgaa tggcataata agttgtgcta
    20041 taataatgta gttgataatg taaaaagaaa gttaatataa ttaatttttt taaataaaga
    20101 gaagaggtga ctataatgca aaaggaaata caaccaaaat acaatccagt tgaagtgcgt
    20161 tgtgcttgtg ggaacacatt tgttgctggt tcaactaagg acgaaataaa agttgaaata
    20221 tgttcagagt gccatccatt ctatacagga aaacaaaaga acatagaaaa aggtggaaga
    20281 atcgacaaat tcaagaaaag attcaaaatg gactaatttt tataaaagtg gagttggagg
    20341 aagccaactc ctattttatt acaaaaaaac attcatattc tactttaaat tgtctttttg
    20401 tattataata ataaatatgt aaaattgagt attctaaata caataaaatg aggtgagtgg
    20461 atggcaaaac aagctgtagg tgggcaagct gttattgaag gtgtaatgat gcagtcaaaa
    20521 gacaaaagag cggtagctgt tagaaaaagt gacggagaaa tagcactaaa agaagataga
    20581 ataaaaagtt gggtaagaga taagaatata gataagatac cttttgttag aggttctttt
    20641 gttatgatag atacaatgat tcaaggcata aaaagtttaa acttttcttc agagtttttt
    20701 atggaagaag ctgaagagga taaatttgat ttatttataa agaaaatttt taaagataag
    20761 gcaaatgata taattataat attttcattg gtcatagcaa tgttactttc tgctggatta
    20821 ttcattttta tacctacatt ggtaggagga gctttttcta aagtaatgcc taatgatttt
    20881 atgcttaatt taatagaagg aatcataagg atagctatat tatttgcata tatagtattg
    20941 atttcacgaa gtaaggatat agaaagagtc tttcaatatc atggagcaga acataaatct
    21001 atatattgct atgaaaatga tttagaactt acagtagaga atgctagaaa atttaaaaga
    21061 ttacatccta gatgtggtac aaatttccta tttatagtta tggctgtatc aataatattg
    21121 tttgcatttt ttggttggcc aaatccaatt cttagaatat ttatgagaat tatatgtgtg
    21181 cctatagtag caggtttatc ttatgaggta attagagttc ttgggaaata tgataatgga
    21241 tttactaaaa ttattgcata tccaggaatg atgttgcaat attttacaac aaaagagcct
    21301 gatgatgagc aattagaggt tgctttggaa gcattgaaag cagtagtaga ttagagaaaa
    21361 ggatatttgt tatgacgata aaagatataa ttattaaata ttcagataaa ctaaaagata
    21421 taagtgatac tccaagatta gatacagagt tattgttaca aaaaacattg ggtgttgata
    21481 gattatatat tcacttaaat ctgaataaag agctaactga agaacaaaaa acaaaattta
    21541 tgggttttgc agaagaaaga ttaaatggaa gaccaatagc atatatagtt gaaaataggg
    21601 agtttatggg attagatttt tttgttaagg aaggcgtatt gattccaaga ccagatacag
    21661 aaactttagt agaagaaata attgaaatat gtagagaaaa aaaagatgta agtatattag
    21721 atataggaac tggttctgga gctattacaa taagtttagc aaagtacatt gaaaattcta
    21781 aaatcatgtc ttttgatata tcagagacag cattagaaat agctaaaaaa aatgctatta
    21841 taaatgaagt tggtgaaaaa ataaagtata ttaattctga tttatttaca gcaataagtg
    21901 atagtaacat aaaattcgat atcatagttt caaatccacc atatattaaa aaacaagata
    21961 tagaaactct gcacaaacaa gttaaagatt atgaaccata taatgctctt gaaggtggag
    22021 aagatggatt agacttttat agaagaataa cagaacaagg taagaaatat ttaaataagt
    22081 gtggaatatt agcatatgaa gttggtcata atcaagctga agatgtaata aatattatga
    22141 aaagtaatgg atataaaaaa atatacacaa agaaggatat tcaaggcatt gatagagttg
    22201 ttataggtta caatatatga tataatcaac aattgatatg ttacatttaa tcgaggtgaa
    22261 agtatgttaa aaaaattaga ggtattggaa gatacatata aagacttaag tgaaaaaata
    22321 ggtgatccgg atgtaataaa tgatcaaaaa gtttggcaga aatatataaa agaacatgct
    22381 gacttggaac ctattgttat gaaatacaga gagtataaaa gcgttttaga tagtataaaa
    22441 gaatcaaaag aaattttaca agaagagtca gatgaagaat taagagaatt agctaaaatg
    22501 gagttagctg agatggaaga aaaagtagca cctctggagg aagaaataaa aatactatta
    22561 ttgcctaaag accctaatga tgataagaac gttatagttg aaattagagg tggagctgga
    22621 ggagatgaag cagctctatt tgctggtgat ttatttagaa tgtactcaag gtatgctgaa
    22681 agaagaagat ggaaaataga attattgagt gcaagtgata ctggagttgg tggatataaa
    22741 gaagtatctt ttatgataaa aggtaaaggt gcttattcaa gattaaagta tgaatctgga
    22801 gttcatagag ttcaaagaat accatcaact gaatctggtg gaagaataca cacatcaact
    22861 tctactgttg cagtattacc agaagttgaa gatgtagagg ttgaaataaa tccaaatgat
    22921 ttaagaatag atgttttccg ttcttcgggt aatggaggac aaagtgtcaa tactactgac
    22981 tctgctgtta gggtaacaca tatacctaca ggtgaagtag tatcttgtca agatggaaaa
    23041 tcacaattaa aaaataaaga gcaagcttta aaaatattaa aagctagact ttacgataaa
    23101 gcattggcag aacaacacaa agatatagca gcagaaagaa aaagccaagt cggaacagga
    23161 gacaggtcag aaagaataag aacatataat ttccctcaag gtagaataag tgaccataga
    23221 ataaatttaa ctttatataa gttagatgca ttcttagatg gagatataga tgaaatgata
    23281 gatgctttaa ttactgttga ccaaacagaa aaaatgactg caatataagt aatgttttaa
    23341 agaagctgtt tcttcctaga aattattttc tattttgaaa cagctatttt gcattttagg
    23401 tcatgttatc atattctatt gtatataatt gttatataca agtataggag ggttatatga
    23461 tactaaaagt aacaataatt gggcttttgg caggtgttat agggacagga ttaggaggag
    23521 taatatcagc catttttaaa agagaggtcg ataaatatct aagttttttt atgggattat
    23581 ctggtggaat aatgttggct gtagtggtgt ttgatttaat gaaagaatcc atggacaaaa
    23641 tggggataat caatacagtt atatttacgt ttgttggagc tctcataact atgtacataa
    23701 aaacaaaatt agatgtgtct ggaaatatgg catctggata tcttatattc ataagtatat
    23761 tactacataa tttaccagaa ggactagcca ttggttcttc ttttatgtca actgaaagtt
    23821 taggtattac acttgctata gttataggtc tacataatat accagaaggg ttagctatgg
    23881 cattaggctt agtttgcaat aaaatgaaac taagtaaagt tattttgttt actgtaatag
    23941 ctggattacc tatgggtcta ggaagttttt taggtgttta ttttggagga gtatttactt
    24001 ctcttatagg agtatttctt gctacagcag gaggtactat gatgtatgtt gtattggaag
    24061 aaatttttcc acattcaaaa agtgtgtatt gtataattgg tttcttgtta ggtacaatga
    24121 ttgtaaatta tatataaaat taaaatgaaa gcaggcatac aaaagatgaa aactattata
    24181 agtaatattg atataaataa tattgattat gaggaaataa aaatacaagc aaaacttctt
    24241 agagaaggta aaacagttat ttttccaact gagacagtat atgggttggg tgcaaatgca
    24301 ttagatgaaa atgcagtaaa gaagatttat gaagctaaag gaagacctag tgacaatcca
    24361 ttgatagtcc atatatatga aaaagaagaa gtatatgact tagccaaaga tataagtgat
    24421 aaagctaaac ttgttataga aaagctttgg ccaggaccta taacaattat attaaataaa
    24481 aaagatataa ttccatacaa aactagtgga gggcttgata cagttgcaat aaggatgcct
    24541 tctaatgtta ttgccagggc aataataaaa gaagctggta tacctattgc agctccttct
    24601 gcaaatatct caggtcgccc atctccaaca aaagcaaaac atgtatatga agagatgaat
    24661 ggaagagtgg atggaatagt tttaggagga gacagtaatt ttggattgga gtcaactgta
    24721 ttggatttaa cagaagagac tcctatgata ctgagaccag gaagtataac taaagaagtt
    24781 ttagaggaac ttcttggaga agtaaagctt gacccatcat taagtaaaaa agaagataat
    24841 caaaaagcta aagctcctgg aatgaaatat aaacattatt ctccaaatgc agatgtatat
    24901 ataatatctg gaaaaagaga aaatgtagca acaaagatta atgatatgat aaaatctaat
    24961 agagacaaag gtctaaagac aggtgtaatg tgtattagta gaaaccgaga gttttatgat
    25021 ggagaagtaa tcgatttagg aaactctctt gaagaagttg caagtaatct gtttgatgct
    25081 cttatagaaa tggacaaaaa gggtgttgat gtcatatatt cagaagagtt ccctaagact
    25141 ggagttggac aagcaattat gaatagacta ctgaagtcag caggatacaa agtgataaag
    25201 gcttaagagg gagttgtaaa atatgaatat acttattgtg tgtacaggta atacttgtag
    25261 aagtcctatg gctgaggcta tactaaggaa agcaattaaa gaatctggaa gaagtattga
    25321 agaatattct atatcatcag caggtatatc tacagcaaat ggaatgggtg cttctgaaaa
    25381 ttctatagaa gtactaaaag aaatcggtat agacttatca aatcatagaa gtaaagtaat
    25441 tactaaaaaa ttaattgatg aatcagatat aatactaaca atgactaaat ctcataaaga
    25501 aattttagta caagcagttc caaaatgcaa agaaaaagtg tatactttta aagggtttgc
    25561 aaataaaaat gaggaagata tatctgaccc atttggtgga aatttggata tttatagaag
    25621 cactatgaga gaaataatgt attctgtcaa cgaaatagta aaaaaaatat gaaacggagg
    25681 ataaacaaat gaagatagga ttaggctgtg accatggagg atataattta aaaaaagaaa
    25741 taataagcta tttagaaagt aaaggtatag aatgtgtaga ttacggaact aataatgcta
    25801 cagattctgt tgattatcct gtttatggag aaatagttgc aaattcagtt ataaataaag
    25861 aagtagatta tggaatatta tgttgtggta cagggatagg aatatcttta gctgctaata
    25921 aggttcctgg gataagatgt gcagttgtat cagatgtatt ttctgcaaaa atgtcaaaag
    25981 cacataatga tgcaaatatg ttatcattag gcgaaagagt tttaggaaaa ggtctagcac
    26041 ttgagatagt tgaagcatgg attaatacgg attttgaagg tgatagacat gctagaagag
    26101 taaacatgat taaatcaata gaagaaaaac ataataaata ataaaaatta cagttttatc
    26161 aggaggaatt atcaaaatga gcaaagtggt agagacaaac catccattaa tacaacataa
    26221 attaacttta atgagagata aaaatactgg ttctaaagac tttagagagc ttttaactga
    26281 aattgcaatg ttaatggggt atgaaataac aaaagatata ccattaaaag atgtagaaat
    26341 agaaactcct attcaaaaaa catcatctaa agtagttgca ggtaaaaaat tagcaataat
    26401 tccaatttta agagcaggtc ttggaatggt agatggatta gtgagcttaa tgccagcagc
    26461 aaaagttgga catgtaggac tatatagaga cccagaaaca ttaaaaccag ttgaatatta
    26521 ttgtaagctt cctcaagaca taggagaaag agatataata gtagttgacc ctatgcttgc
    26581 aacagggggt tctgctgtag cagctataga tttacttaag tctaaaggag ctaaaagtat
    26641 aaaattagct aatttagtag ctgcaccaga aggtatagca gaagtgcaaa aatatcatga
    26701 tgatgttgat atatatgttg catcagttga cgaaagatta aatgaacatg gatatataat
    26761 tcctggtctt ggtgatgctg gagatagatt gtttggaact aaataaaaat aaataactat
    26821 aaatatttat tagggacttt cttatatagt gtatataatt ttatgtaaga aggttctttt
    26881 ttatatattt tttctcaaaa taggaattat tgaattgata aatttctcga attgagaata
    26941 aaaagttttt accaattaaa aaagattgct tattttacaa ggttttatag aatagtagtc
    27001 tattttaatg tggcatgtta gttgcttacg attaagatta taggtattaa gtgtattagt
    27061 taaattgaga attgaatatt aataatatta ttgggagtgg taatacatga agaattgtat
    27121 agtagtaaga ggggctggag atttagcaac agcagttatt cataaattac acaacagtgg
    27181 ttttaaagta gttgcgttag aaatagaaga gccattagct ataagaaggc aaatttcttt
    27241 ttgtgaggct gtttatgaaa aaaaagtaac tgttgaaggt gtagaatgta tattttgcaa
    27301 tactgaaaaa gaaatagaag aagttttatc tcaacataaa gtagccttga ttatagaccc
    27361 aaaaggagaa aaattatcta aattaaatcc aatcgttttg gttgatggaa tactcgctaa
    27421 gaaaaactta ggtacaacta aagatatggc accacttaca atagctttag gtcctggatt
    27481 ttgtgcagga gaagatgtag atgttgttat agagacaatg agaggtcata atctaggaag
    27541 aataataact aatggtagag cagaaaaaaa tacaggagta ccaggagcaa taaaaggttt
    27601 ttctaaagag agagttatgc attcacagta tgatggcata atggaaaatg tatgtaagat
    27661 aggtgatata gttgaaaagg gagacctact ttgctatatt aataattatg aaaaaaaaca
    27721 tgaagttaga gcaacaatat ctggagtcct tagaggttta ttaaaggatg gagcaaaggt
    27781 aaacaaaaat ttaaaaatac tagacataga ccctagaaaa gaagaagtta agaatgcttt
    27841 tacaatatca gataaagcta gatgcatagc tggaggggtt ttagaagcca tactaaacaa
    27901 tgggatttat ccccaaaatt aatttatatt agataaagct attaatattt tattaagatg
    27961 atataataaa tttaatagat taaagagagc atatgacggg tatttaaaat ttaaatggta
    28021 aaaatgctct ttttttattg gacattcaat taacatgtgt atataatata aaaataagta
    28081 tttatttagg ttgaaatcta ataaatatat aataccagta cataagcaaa gaggagaaaa
    28141 gtaaattatg agaccatcat gggatgaata cttcatggaa attgcagaag tagtgaaaaa
    28201 gcgttcaact tgtattagaa gacaagtagg agctgtaatt gtaagagaca aacaaatatt
    28261 aacaactgga tataatggtt ctccgagaaa tcttgaacac tgtgaaaata ttggatgtaa
    28321 gaggcaggaa ttaaatattc cctcaggaga aaggcatgaa ttatgcagag cattacatgc
    28381 tgaacaaaat gcgataatac aagctgctca taatggtatt agtgtagatg gggcaacttt
    28441 gtatgttact acaagacctt gtgttttgtg tgcaaaaatg tgcataaatg caggtatatt
    28501 gaaaattgtt tatgaaggtg attatccaga tgatatgtct acagaattat taaaagaagc
    28561 aggaatagaa ctcataaaat tttagtggat aaaatttata agacaataaa tgtcaaatta
    28621 tgacactgaa atacaatggt atcaaatgat taaaagaaag tatttgaaaa atcatcagat
    28681 tagtagtaag atatatttta taattaaata aatttatgag atagattaat tacatgttat
    28741 ttattttata ataatgaggg atactattta tgagtaaaag aaatacacat acagaaattg
    28801 caaagttgtt tagtttaata agtcaaatag gattgatgat tgtaatatca atattaggat
    28861 gtactttctt aggaaaattt ttagatagta aattgaatac tactccagtt ctgacaataa
    28921 tatttttatt gttgggtgtt ggaggtgctt ttagtggtgt ttataaaact ctaatagtat
    28981 atacacgaag gaagtgatat attggatata aaattactta accaggtaag gtcagtaaca
    29041 aaaggtatag taatatttga tgtagtttta attgtgctaa tgttgataac atcaacatta
    29101 agtaaaccag tattattagg acttatattt ggctctataa tagctatgtt aaattttaga
    29161 ttattggcga tatcactgga aaaatcagtg acatttcctg ctgggaaggc acaagcttat
    29221 gcatcagcac aatatgctgt tagattattt ataatggcag ttgttctaat tgcatcagct
    29281 acagtaccac atttaaatat aatcggaaca gcattgggat tattgagcac aaagtttgtc
    29341 atattatcaa aaaactttat aaataaatta aagaggaagg aggcataagt atgaatcaag
    29401 cgcattatta tctcatagga aactttaaga ttagtgaaac aatactagta agttggggaa
    29461 taatggcctt tttagcgata gcatcttatt ttttgactag aaacttaaaa aaagttccaa
    29521 ctagtaaagt tcaaatattc ttagaatttg cagtaggtgg tcttgctaaa atggttacag
    29581 atgttatggg taaaaataca gtaaaaagaa tgccatatat gataccatac ataggaagct
    29641 tgtttttatt tttcgcatgt tcaaatttaa gcccattact tggattaagg tcaccaacaa
    29701 ccgacttaga cacaactgct gcatgggcaa tgattacatt ttttatgatt tattttgcag
    29761 gagttaaatt taaggggtta tcatacttta aagaattggc tcaaccactt gccataatgt
    29821 taccaatcaa tattataggt gagttagcta gaccaatatc attaagtttc cgtccatttg
    29881 ggaatatttt aggtggaaca ataataatgg gtctgtttta tcaactttta ggttttatat
    29941 ctagtttaat accaggatta tcaataccaa ttggagaaat attaatacca gtcccattac
    30001 acttgtactt tgacttattt gcaggattgt tacaagcata tatatttata atgttaacaa
    30061 tggtatttat atcaagtgca ggagagttag attaagaaat caacttatat taagataaaa
    30121 ataaaataat ttaaaaaatt aaagttttaa ggaggaaata attatggaaa cagcaatagt
    30181 agcggcagca tcagcaatag gagcagggat agcagttgca acaggtatag gtgcaggtat
    30241 aggtcaaggt atagcagcag ctaaagctgc agaagcagta ggaaaccagc cagaagctaa
    30301 aggtgatata acatcaactt tattactagg agttgctata gcagagtctt cagcaatata
    30361 tggtttggtt atatcaataa tacttctatt cgtaaatcca ttctttaaat acttaggaat
    30421 gtagatttag tccttaaata ttttgagata ggtttatagg taaatcatct caaacttata
    30481 atcatgagat gaactttatc atctcgtgat tataagtagt tatgatttat ggaaaggagg
    30541 ataattatgg aaaaagcact agtaggtata acgtgggaat ttgtgttcca aattgttaat
    30601 acttttataa tatttttact tctgagaaag cttttattca agccagtatt aaatataatt
    30661 gaatcaagag aaaatgatat taaaagtgat ttggcagaag gcgaaaaagc aaaaaatgaa
    30721 ggtttagctc tgaaaaaaga atatgagtca aaaatcaatt ttgccaaaga tgaaggtcaa
    30781 gaaatcataa aacaagcaac tataagagca gagcagaaat cagatgatat agtaaatact
    30841 gcaaagaaag atgctcttga tataaaagaa aaagcaaaca aagatataga acaagaaaga
    30901 caaaaggtta taaatgaaat caaaaatgat atatctaaca tagcactact tgcagcatct
    30961 aaagttattg aaaaagattt agataaatct aaacatgaag aattgataga aaactttata
    31021 aaagaggtag gggaagctaa atgataaatg taatagcaaa tagatatgcc gaagccctat
    31081 ttcaattagg agaagaagaa aattcaactg atgtcttatt caaagaacta gaaaaagtag
    31141 tagatatgat gactaaagtt agtaaagatt tctacaaagt tttaaaatca ccattagttt
    31201 caaaatcaga aaagaaaaac ttggtagaaa ttatttttag taaggaagta agttctaaca
    31261 taaaaaactt tttaaaagtt ttagtagata aagatagaat atcatattta gaagatatcg
    31321 aattagctta taaagaatta ttaaataaga aaaacaatgt aattgatgga gtagcaataa
    31381 gtgctattcc aatgagtgaa acagatatta aagaacttga agtcaaactt tcaaataaat
    31441 acaacaaaaa tgttactata gaaaatgttg ttgacaagac tatactaggg ggagttctag
    31501 ttagaattgg aaatgaacaa atagatggca ctgtgaaaac tcgtcttgat aagatgaaag
    31561 aaaagctatc tgaagtaata tcttaagaat ggcggtgaac ccatgaactt aaaacctgaa
    31621 gaaataagtt ctataattaa acagcaaata aaaaattatg agaataaagt tgagttgaca
    31681 gatacaggta gtgttttaac agttggggat ggtatagcga gtgtatatgg attagaaaaa
    31741 gctatgtctg gtgagttgct agagttccca ggtgaaatat atggtatggc acttaacttg
    31801 gaagaagaag tagttggagc tgttatcctt ggagatgaca gtgaaataaa agaaggcgat
    31861 atagttaaga gaactggaag aatagttgaa gttccagttg gagaagcttt aataggtaga
    31921 gttgtaaact cattaggtca gccaatagat ggaaaaggac ctatagcgta cacaaaaact
    31981 agaccagttg aatctgaggc acctggtata atagatagaa ggtctgttta tgaaccatta
    32041 caaacaggaa tcaaatcaat agattcaatg ataccaattg gtagaggtca aagagagtta
    32101 ataataggtg acagacaaac tggtaaaaca tctatagtta ttgacactat actaaatcaa
    32161 aaaggaaaag atgttatctg tatatatgta gcaattggac aaaaacgttc tactatagct
    32221 caattagtta gctctttaga aaaaggtgga gctttggatt atacaatagt agtttcagca
    32281 acagcatctg aatcagcacc acttcaatat atagcaccat atgctggagc agctatgggt
    32341 gaagaattta tgtacaatgg gaaacatgta cttatagtgt atgatgattt aagtaaacaa
    32401 gctgttgcat atagagaaat gtcattgtta cttagaagac caccaggacg tgaggcatat
    32461 cctggagatg tattctattt acattcaaga ttacttgaaa gagcagctaa gttatctgat
    32521 gaattgggtg gaggttcaat gactgccttg cctataatag aaactcaagc tggtgacgtt
    32581 tctgcatata taccaacaaa tgttatatct ataacagatg gtcaaatata cttacaacca
    32641 gagttattct attcaggtgt aagaccagca gttgaccctg gtatatcagt atcaagggtt
    32701 ggtggttctg cgcaaattaa agcaatgaaa aaagttgcag gaacattaaa acttgcatat
    32761 tcacaatata gagaacttgc agcattctca cagtttggtt ctgacttaga tgaagatact
    32821 aaaaagagac ttgctcaagg tgaaagaatc gttgagatat taaaacaagg tgagcatcaa
    32881 ccaataaaag ttgaaaatca agttatgata atatatgctg ttataaataa tcatttagag
    32941 gatattccga tagataatat agctagattt gaatcagaat tgtatgcatt tgtagataat
    33001 aattatccgg aaatatcaag aaaaatatta ggtggagaag acttcactca tgatttaaca
    33061 gatgcaataa atgaatttaa agaaaagttt gttgttgaag tataatcctt tttaaaaaag
    33121 gaggtaaatt aattggcagg agctggaatc aaagcaatca aaacacgtat gggaactgtt
    33181 gaaagtacaa agcaaataac taaagcaatg gaattagttg catcttctaa gcttagaaaa
    33241 gcaaaagaaa aggctgaaga ttcaagagaa tattttaata ctttatacaa tacagtaaaa
    33301 gatattgccc aaacaacacg tggaattaga aatgaatttt taaaagaaag agaagtaaaa
    33361 aataaatgct atgtagtagt agctggagac agaggacttg caggtggata caattctaat
    33421 gtattaaagg aatctgtctc tcatatgaaa ggtaaaaagg aaacagtgat tactgttgga
    33481 aagaaagcac atgaattttt ttctaaaaga aactacaata tagcaaaaga tataaatagt
    33541 gttgaagctt gtggatatga agatgcgaca gaaatttcac aaactattat tgacctgtat
    33601 aaaaaaggtg aagtagatga agtttatatt gcttatacaa aatttaaatc tgctttatca
    33661 caagaagttg aaattttgaa attgcttcca ttagtctttg aaaaagatac agacgaacaa
    33721 ggaatggcag taaaagaaga aaaacaaaat agatcaaaag tacaatatat accatcagca
    33781 gaagcagttt taagttatat tgtacctcag tttatatcag gtacagtata tggtggaatt
    33841 ttggaatctt ttgcatcaga gcaaggtgct agaagaactg caatggaatc agccacagac
    33901 aatgcgaatg aaatgctatc taatttagag ttaagttata acagagcaag acagtcagct
    33961 gtaactcaag aaataacaga gatagttggt ggagttgaag ctctaaaata gggaggttag
    34021 gaaatggcaa atgtaggtaa agtagtacag attgtaggag ctgtactaga tgttaagttt
    34081 gatggtgaac aaagcctacc taacttatta aatgcgttag taataaagtt aggagataaa
    34141 gaaatagttg cagaagttgc tcaacatata ggtgatgata ctgtaagatg tatagctatg
    34201 agtgcaacag acggacttgt aagaggtatg gaagttgtag atacaggagg gccaataagt
    34261 gttcctgttg gagatgagac tttaggtaga atattcaatg ttcttggaaa accagttgat
    34321 gggaaaccag ctccaaaatc tgcacctaaa ttacctatac atagaccagc accggcgtat
    34381 gatgaactag aaactacagc agagatacta gaaactggta ttaaggtagt tgacttacta
    34441 gctccatatc taaaaggtgg aaaaataggt ctatttggag gagcaggagt aggaaagaca
    34501 gttctaatac aagagcttat aaacaatatt gctaagcaac atggtggtat ttctgtattt
    34561 tcaggagtag gagaaagaac aagagaaggt aacgaccttt atggcgaaat gagtgagtct
    34621 ggagttataa ataaaacagc tctagtattt ggtcaaatga atgaaccacc tggagcgaga
    34681 atgagagttg ctttaactgg acttacaatg gcagaacatt ttagagatga gcaaggacaa
    34741 gacgttttac ttttcgttga taatatattc cgtttcacac aagctggttc agaagtttca
    34801 gcacttctag gacgtatgcc atcagctgtt ggttatcagc caacattagc tactgaaatg
    34861 ggtgcacttc aagagagaat aacatcaact aagaaaggtt caataacatc tgttcaagca
    34921 gtatatgtac cagcagatga cttaactgac ccagcaccag ctacaacatt ctctcactta
    34981 gatgcaaaaa cagttttatc aagacaaata tcatcactgg gtatatatcc agcagttgac
    35041 cctcttgaat ctacatcaag gattcttgac cctagtatag taggaaaaga acattatgaa
    35101 gtagctagag gtgtacaatc aatacttcaa agatataaag aacttcaaga tataattgct
    35161 atacttggta tggatgaatt atctgatgaa gataagttaa tagttgcacg tgctagaaag
    35221 atacaaagat tcttatctca atcattcaca gttgctgagc aatttacagg aaatccaggt
    35281 caatatgtac ctgttaaaga gactgtaaga ggatttaaag aaattcttga agggaaacat
    35341 gatgacttac cagagtcagc attcttattt gtaggaacaa tagaagacgc tgttagaaaa
    35401 gcgaagggaa gtatgtagtc atggccagtg aattttcatt gaaagttgta actccaagta
    35461 atacttttta tgatggagat gttgaaatga tagtagttag aactacaaaa ggagacaaag
    35521 gggtgttgaa aggtcatata ccatttgttt caggtatacc tgtaggaact ttaaagataa
    35581 aaaaagaggg aagttttaaa gaggcgaaaa tagcagaagg attcattaat gtaactcaag
    35641 ataaaactgt catattaaca gaatctgctg agtggattta ataaattaat ttagagtata
    35701 aaaagtcatt ttgaccaaaa aaacttttgg gtaaaatggc ttttttgtgt tttggtgtta
    35761 ataaaataac gataaaattg taagttttta gtgataataa attcacaaaa tgtttaaatt
    35821 tactgccaaa ttatgttgta attaataaga taaagaagat ttagatagct tttatgtaag
    35881 tgaaaaagtc ttcatataag aaatacatat aaaattttta taaaatttaa aaaatttgct
    35941 tagttgtgta gtataataaa taatataata gatttgtata taatattcat tacaaataat
    36001 tttaaaagtt acagttttat tataatgatt attaaattaa gataagtaac ataaactaaa
    36061 taataatgtg tattatcgat agtaaattta taaattcatt attaattatt gaaagaagta
    36121 ttataaattt tctggaggaa caatgaatat atttgatatt ggcgtagata taattgaaat
    36181 tgatagaata agaaaggctg tagacaaaaa taataggttt ttggagaaga tatttacaga
    36241 tagagaaata gagtatttta attctaagaa ttttaaagca gaaagtatag caggtaattt
    36301 cgcagccaag gaagctataa gtaaatctat aggtacagga attagattgt ttaattttaa
    36361 agatatagaa gttctcagaa atgaaatggg aaaaccaata gtaaaaactt ataataatct
    36421 agcaaaaatg tgcatagatt ataatgtttt agaaataaaa gtttcaatat cacattcaaa
    36481 agattatgct atagcaaatg cgataaccat aattaaggac taaggagtga ttacattatg
    36541 atggataaaa cagcaaaaga aataatgaca acagatgtaa tagtagcaaa acaagatgat
    36601 agtatagcag atgtagctaa tatgctaatt gcagaaaaaa taggaggtct gccagttgta
    36661 gattcagaga atagagttgt tggaataatt tctgaaacag atattcttaa aaaagaaaaa
    36721 tatatagaag cacctctcta tataaatcta ttacaaggat tgattttctt agatgactta
    36781 aaaaaagttg aaaaagatat aaaacaagta gctgcttata aggttggaga acttatgtct
    36841 aaagatataa taaaggttca tgaagatgat aagtttgatg atgtagctaa tgtaatgatt
    36901 aaaaaatcaa taaacagagt tcctgtagta gatgatgaca acaaacttaa aggtataatt
    36961 tgtagatatg atataataaa agctttatac aatgaataaa ctgaagtagg aggtgtgatg
    37021 tgagaaaaaa gtggaccata gtatgtatta tgttcttggc actggttata attgtaatag
    37081 gatgccaaaa acgacagtcc acaaaagaag aagtatacaa agattttcaa aagcagattt
    37141 ctgatatgaa ttattacagt tgtaaagctg aagtagaagt agttggaaat aaaagtccac
    37201 acaattatgt tttaattcat acttataaaa aaaccgataa ctataaatta gaagtaattt
    37261 ccccaaaaca tttaaaaggg aaaagtatag aatatcaagg tgataaaata ttagttaaaa
    37321 atccaaagat tagtgatgta gtggagttac ctaatacagg aaaaaataat caatatcttt
    37381 ttgtaggaga ctttataaaa aactatcttc aaaatgagga gatgaaagta aagttatcca
    37441 aaggacattt ggttttagag acttttattc ctggggataa taaatatttt aacaagcaag
    37501 tattatatgt aaatgctgac acaaaaaatc ctgaaaaaat ggaagtgtta gataaagagg
    37561 gagtgccaag atttacagta aaatacaaag attttgaata cagaaactaa gggggactaa
    37621 agacatgcaa aaaataacag tgcctacatg ggcagagata aatctagata acttaagatt
    37681 taacttaaat aatattaaaa atttattaga agaagatatt aagatttgtg gagtaataaa
    37741 agctgatgca tatggacatg gtgcagtaga agttgcaaaa ttgctagaaa aagaaaaagt
    37801 agattactta gcagtagcaa gaactgctga aggaattgaa cttagacaaa atggcataac
    37861 acttcctatt ttgaacttgg gatatactcc agacgaagct tttgaagatt ctataaaaaa
    37921 taaaataact atgacagttt attctttaga aacagcacaa aagataaatg aaattgcaaa
    37981 atctttagga gaaaaagcct gtgttcatgt taaaatagac tcagggatga ctagaatagg
    38041 tttccaacct aatgaggagt cagtacagga aataatagaa ttaaataaat tagaatatat
    38101 agatttagaa ggtatgttta ctcattttgc tacagctgat gaagtaagta aagagtacac
    38161 ttataaacaa gctaataatt ataaatttat gtctgataaa ttagatgagg ctggtgtaaa
    38221 aatagctata aaacatgtat caaacagtgc agctattatg gattgccctg atttaagatt
    38281 aaatatggta agagcaggaa taatattata tggtcattat ccatctgatg atgtatttaa
    38341 agatagatta gaattaagac cagccatgaa attaaaatca aaaatcggac atataaaaca
    38401 agttgaacca ggtgtaggaa taagttatgg actaaaatac acaactacag gtaaagaaac
    38461 aatagctaca gttccaatag gatacgcaga tggatttact agaatccaaa aaaatccaaa
    38521 ggttcttatt aagggagaag tgtttgatgt agttggtaga atatgtatgg atcaaataat
    38581 ggttagaatt gacaaagata tagacataaa agttggagat gaggttatac tatttggaga
    38641 aggcgaagtt acagctgagc gtatagctaa agacttagga actataaact atgaagtgtt
    38701 atgtatgata tcaagaagag ttgaccgtgt ttatatggaa aataatgagc ttgtacaaat
    38761 aaacagttat ttgctaaaat aaaaccatga tattataatc tgggaggcga ttttgtgtac
    38821 gataagagta aagaaaaaat agaagttact ttacctgact ctctgatttc tgaagttgat
    38881 agtatagttc agatggagaa cagtaacaga gaagattttg caaaagctgc ttttcagttc
    38941 tatataactc agaaaaagaa aattcatatt aaagaatcta tgaaaacggg ttatagagaa
    39001 atgggtcaaa ttaatctgtc actagctgaa ttaggtatga cagttgaaga agtatcttct
    39061 gatgataaaa atgaagggaa gattgcccaa ggtgagtact aattgtgaaa taaagcgtgg
    39121 tgatttatat tacgctgatt taagtccagt tgttggttca gagcagggag gcgttagacc
    39181 tgttctgata atacaaaatg atgtaggtaa taaatatagt cctacagtta tagtatcagc
    39241 tataacatct cagattaata aagccaaatt acctactcat gtagagataa gttccaatga
    39301 atatgggctt aataaagact cagtaattct tttagagcaa attagaacta tcgataaaaa
    39361 gagactgagg gaaaaaattg gttgtctaga tgaaaatatg atgataaaag tagatgatgg
    39421 acttcaaata agtttaggat tgtttacttt ttaaagaggg atttgtcttt tattagacaa
    39481 tccctttttt cttttaaata tctcttaagg tttaatttta atgtactatg ttaataaaat
    39541 taaaaataac gcaaaaaaat agcataaaag tgatataata atatggaatt gatataaaat
    39601 tacgaactca agggaggtaa ttgcatgaga gaccataaag gattaaatga gattgcgcgt
    39661 atcataagaa gagatatagt ttcaatgata catagagcca aatctggaca tccaggaggg
    39721 tcattatcag tagttgaaat acttactgcg ttatactttg atgaaatgaa tgtggattct
    39781 tcaaatccta aaatggagga tagagataga tttgtattat caaaaggaca tgctgcacca
    39841 gcattatatg ctacattggc tgaaaaaggt tattttgata aggaagaact gaatggatta
    39901 aggaagatag gaaggatgtt acaaggtcat ccagacatga aaggcacgcc aggtgtggaa
    39961 atatctactg gttctttagg tcaaggtttt tcagttgcat gtggtatggc tatggcatca
    40021 aagttggata atgctccgtg gaatgtgtat actttgcttg gtgatggtga ggttcaagaa
    40081 ggaattgttt gggaagctgc tatgagtgct gcacattata aacttgataa tttaattgct
    40141 tttttagata ataatggact tcaaatagat ggagatatag aaagcgtaat gagtttaggt
    40201 tcaatagttg ataaatttaa agcttttggt tggaatgtaa ttgagataga tggacatgat
    40261 tttgaccaaa tatttgcggc tcttgatata gctaaatcaa cagttaaaaa acctacaatg
    40321 atagttgcaa aaactataaa aggcaaagga atatctttta tggaaaatca agcaggatgg
    40381 catgggacag ctccaagtga tgaggagcta gaaaaagcct tgcttgaatt aggaggtgct
    40441 gacaatgagt aaaatggcga cgagagaagc atatggtaaa gcattagtaa aattaggtca
    40501 aataaatgac aatgttgtag tattagatgc agatttatca aagtctacaa aaacacatga
    40561 tttttacaag tcatttccag atagattttt taatatgggt atagctgaac aaaatttaat
    40621 aggcgctgct tgtggattat caactgctgg taaaatacct tttgcaagta cttttgctat
    40681 gtttgctacg ggaagggctt ttgagattat aagaaattca gtttgttatc caaagctaaa
    40741 tgtaaaaata tgtgctactc atgcaggtct tactgttgga gaggatggag catctcatga
    40801 aagtgtggaa gatattgcta taatgagagc tataccaaat atgacagttc ttgttccagc
    40861 tgatggagtt gaaacagaaa aaattatttt tgaaatagct aagtacaatg gacctgttta
    40921 tgttagatta gggagaagtt cagtaccagt tttatttgat gaagattata aatttgaaat
    40981 aggaaaaggt acagtcttaa gagaaggaaa ggatgtatca attatagctt gtggaattat
    41041 ggtaaatgaa gctctattag cacaagaaaa gttgcaagaa gaaggtataa gtgctagagt
    41101 tataaacatg tcctcgataa aacctataga caaagattta atattagaat cagcaaaaga
    41161 aacgaatgct attgtaactg tagaagaaca tagcataatt gggggattag gttctgctgt
    41221 aagtgaggta gtaggagagt cttgtccaac cattgtaaag aaagttggaa ttaaagatac
    41281 atttggagaa tctggtactc caaatgaatt gttaaaaaaa tatgaattaa cttgtgatga
    41341 cataataaag actgttaaag aagttattat tgcaaaaaga atgtaagaga tataaattat
    41401 tcatgtttgt taataatata atatatttaa aagagaggta taaagtgtta tattaattta
    41461 tatctctctt ttttttatat gatatcaata tatcaaaaat gtaataaaat attcaaattt
    41521 ttacaaaaat agagaaaata ttcaaatata tattgactat tcagaaaaat atgactaata
    41581 tataatttat agagaaacta attttatata taatgactac ttagataaaa gaatctaaaa
    41641 aaaattatga gaggaggagt agggtataaa atcagattat aagttttaag ttattatact
    41701 aaaaagctat aaaacagtta tcatgtattt tatgttaaag gaaattgtag gtagtttatc
    41761 gaattgttta atcagaagaa aaaatttcac atggggaggt aactgagata tgtcagaaaa
    41821 agtaaatgtt aaaacagtca tcagtttcgc aggtgcgtat gtagcaacag taatcggttc
    41881 tggatttgca acaggacagg aaataatgca attcttctct ttttatggtt ttgcaggtat
    41941 tattggtggg ataatttcaa tggtattatt ctcatggatg ggtgcttcag ttatgtcaaa
    42001 aggtaaagag cttcaactta aagaacctat caaaatttat cgtgtttatt gtggtaagta
    42061 tttaggaata ttctttgaat ggtttggacc tttattctta tttggtgttt tcgtagtaat
    42121 gatttcagga gcaggagcaa ctttaactga atactatgga ttaaatccat ttgtaggtag
    42181 agtaggtatg gcagtagtag cattattaac tgtttcacta ggtcttgata aattatcaaa
    42241 gatactaggt ggaatagggc ctataataat aatctttaca cttttagttg gtggtataag
    42301 tttagctaaa aacataggta atataggaga agctgcacaa gttcttagca ctgtaaagat
    42361 agctaaacca gttccaaatg cctatctatc tggtgtaata tatactactt ataacacaat
    42421 tgttgttatg gcattcttaa ctggattagg agcaagtgct gctaacaaaa aagaagctat
    42481 ttgtggtggt atattaggtg gagtagcact tatggctgca gctataatga tgcacttagc
    42541 tattctttct gatataggaa acttatattc aaaagcaata ccatcattat tcttagcaga
    42601 taagatttca ccagtaattg gagttttatt ctcaataatt cttatattag gtatatatac
    42661 aacagctgta cctttattat ggtcagtaac taatagattt gtagatgata aacatcctaa
    42721 gtttaaatta ataactttaa tagcagctat attaggtctt ataggtggct tcctaccatt
    42781 tgataagctg gttggtattt tatatccata tactggatat atgggcgtca taatactggt
    42841 ttgtgttctt tatagacaga ttacaaaaac atctggatat caagaaggaa tcagtgacaa
    42901 ataagtattt tttaatattg taaactcaag agctatatga aaacaatgaa aatgttttca
    42961 tatagctctt tttatttttt agctcgttta ttatgaaata gaaaaaacat gttttttagt
    43021 tacattacat atatttataa ttatgtataa atatatacta atcaataatt tagagtctaa
    43081 gataagttta taaatcatat atttagaata tatgggagga gtgataagat tgaatgtaaa
    43141 gtttaatatt aaaggtataa tttatggagt tatagctctt gtattcgtta tagggggatt
    43201 tatattcata ccaagtatgt ttgtagagaa atctaaaccc atagattata cagtattaca
    43261 aagaaattca ataccagaaa agatattaga tgtgatggat aagtatacaa atgaagaaag
    43321 agcattagcg gctaaaatcg ataacaaaat atacattata gtgactagag gaaataataa
    43381 atatggtata gaaatggaca aaatagaaag tgtggttgag gaaggaaagg atgttttgag
    43441 agtcaagata aagtataaag ataaggaaga ttcttttcca tacatagttg ttgaaacaaa
    43501 tatgagtgaa cttccagata gaatagaatt aaatgcgacg aaataagaag gtattttata
    43561 attaaataac tgcatattgt acaataaagt catggttttt attaatgtaa gtaaattgtg
    43621 tatggttctt attgttgtaa aagccatgac ttaaaatgct tataaagtaa gatattaaca
    43681 gtagtattta ttactacatt aaatggggtc tggaaatact taatattcta actatttgga
    43741 gtgataattc tagtttcagt aactatgtag tctaattgtg catcatgagt ttcttttgga
    43801 atagaatcga atatctggaa gtcaaatgct atgccgatag ttatagcatc ttttcttaag
    43861 ttttctaaaa atctatcata aaatcctcca ccatatccta atctatagca atgtatatca
    43921 tatgctacag ctggtacaat tacaacatct aaagccttaa tatcaattgt aggtgatttt
    43981 tcgttaggtt ctcttattcc atatgctcca attctcaatc cctcttttaa atctgttatt
    44041 tgagtaggga ttaatgtatg attttctttt attgtgatgg gacttgatac tattttcttt
    44101 agagacaaaa gttttttgat taaattatct gtttgaactt cattgttaaa atttagataa
    44161 agcattatat tagttgcatt tcttatatct tccatttgaa gtaatttttc agttattaaa
    44221 tttgaattac taagtacaaa gtttgcactc ttattttttc ttgattcaat aacttttttt
    44281 ctaaattctt ttttcataaa ataattcctc ccttaatatg ttataatgtg tacagcaatt
    44341 gtatttaata taaattttag tataatttat tgtaataaag caatatattt gggtaaatgt
    44401 aataaaaata acagctagtc atataataat aatagtttat gtaaaaggag atgtatcttg
    44461 tgatttcaaa gaagaaagct atttttttag gggtgatttt agtaattata acagctatgg
    44521 ttacatcagc atttcagttg acattgggga ataaagttgt aatatcgaaa gaactctatg
    44581 aagactataa aaaatatgat aagttgttag gactagaatc tattataaaa caagattttt
    44641 ataaaaaagt atcagatact gatttggtaa atggagcagc aaaaggtcta tttctaggaa
    44701 caaatgataa gtattctggt tattatacta aagatgaaat ggagaatcta attaatgata
    44761 gtgaaggttc ttatgttggt gtaggtatgt atataggggc atcaaaagat ggtggcttag
    44821 tagtagttcc catgaaagat tctccagcag agaaagcagg tgtaaaatca ggggataaat
    44881 tagtaaaagt caatggaaaa tctgtctcat ataaaaattc agatgaagct gttcgtatga
    44941 tgaagggtaa aaaaggtaaa acagttgaac tgactatatt aagagaagat aaacaattga
    45001 actttaaagt aaaaacagac cagataattg agaagagtat agaaagtaaa gtaattgata
    45061 atgatttagg atatatagaa attacccaat ttatatcaag tacatatact gactttgata
    45121 aagctttaaa agaattaaaa gcaaaaaata tcaagggatt agtaatagac cttagaaata
    45181 atcctggtgg aatgctagat atatgtaaag aagttgctga tgagttaatt ggtgaaggaa
    45241 ccatagtata tacaaaagat aataagggaa atactgagta cttgaaatct gataaagaaa
    45301 aattgggact tccaatagta gtgttaacta atggagaaag tgcttctgct gctgaaatat
    45361 taacagctgc aatcgttgat aataaagaag gtatttctgt agggactaca acttttggta
    45421 aagggttggt acaatcagtt gtaagattga aggatggaac aggatacaag ttaactactg
    45481 ctcaatattt tacacctaat ggagattata tcaatgaaaa aggtataaag cctactattg
    45541 aagaaaaaga tgaaaataaa cagttagatg tagcaactaa gtggcttaga gaacagattg
    45601 ataaatagga tttaattttt ataaacttat actataaaat aaagaacatt ataaaagaga
    45661 tgtctcaaaa tgaacttttt agttcatgag gcactctttt tgtatgaaat caaatgtatt
    45721 ttcatcattt taacaatgaa aaagaggctt atctctattt ttaggaagcc tctataactt
    45781 tatattgaat ctatttatta actttgtaca tacagatacc atttatataa gtttcgtgta
    45841 tattaaagtc attatctgtt actataaagt ctgcatccat accttgatat atagagccac
    45901 agacattatc aatattacaa cttaaagcag ggatatatgt agccattttt attgcatctt
    45961 ctaaagttga tatgttccaa tcaactatat ttctcacagc cttgtccata gttaatacac
    46021 ttccagctaa actcccacta tttaatcttg caacactatt ttttacattt acattaaact
    46081 taccaagagt atattttcca tcctccatag caccagctct catacaatct gtaattaaag
    46141 ctatgttatt actagactta gcatccatca ttattttaac agctattggg cttacatgat
    46201 gaccatcaca aattaattca gcataagtat cttttagtac catagcagcc ccaaccattc
    46261 caggttctct atgatttaaa ggactcatcc cattatatgt atgtacaaac actttagcac
    46321 ctgaattaac gacttgttta acttgctcaa atgtagctga actatgacca agagaaactg
    46381 atatattatt tttagttgca tatcttgtta attctattga acctttacgt tctggagcaa
    46441 ttgctatttt ccttattaaa tttttcgatt ttttttgcca taattttaat atattgatgt
    46501 cagggtcttt aaaatatttt tcattttgag cgcccttata tttttctgta aaaaatggac
    46561 cttctaaata aatcccttgt atttttgcac cctcaactcg attgaagcta ttgcttatat
    46621 tttctacaga atcacttagt gtatctacag aatcagttaa agttgtaggt aggaaagatg
    46681 ttactccata ttttaataag ccttttgaaa ttgtgtttat tgcttcaaat gtattgtcca
    46741 tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac taagccagga gcaatatatt
    46801 tacctgaaaa atctataaca ttatcagtat tattaggtct ttcttgttga aattcaccga
    46861 atttgccatt aattatacta aggtaacctt tttctttgat tccatgtttc ataaaaaatt
    46921 tatctgcata aataaaactt ttcaaaataa cctccttata attatactat cattgtggca
    46981 ttgacgctta taatatcatt ctgttttctt gaaagctatt taacttacta tcttacatgt
    47041 cttactctat atacaaattt atcgcttcta gcgacactca aagtaaattc aataactata
    47101 ttgtcatcat tatatgtggt acgaagtaat ttcagacaag gtgaatctcc tggtacttcc
    47161 aataacttag cttccttatc agagattatt ccagctgaaa attcttcatc agcaacttta
    47221 ataatctcgc cataatcttc tctaaatatt tcatataaag gtttttgttc gattttttct
    47281 tttgtcaatc ctataaattc tttcatagga agatatgttc tttcaagcat cataggaagg
    47341 tcatcagcaa gtcttaaacg cattattttt atcatttttt caccagtagt tatacctaat
    47401 ttttcagaga aatactttgt actttctact acctcaaatg ataatacctt tgtacctggc
    47461 ttttttcctt gtgttctcat ataatctgta aaactataac tttccattaa gttttgagaa
    47521 ttttgactta gtgcagcaac aaaagttcct tttccatgtc ttttgtatat atatcccata
    47581 tgttccaact cagccatagc tagtctaact gtggttctac ttactccata ttcatcacaa
    47641 atttttcttt cggaaagcat tctatcgttt ggttccatct cattttcaat cttagttctt
    47701 aatatctcaa caagctggtc atatattggt cttttatcat ttctttttat cataaaaata
    47761 tagtcctttc taagtggtta ttaccacaat ttgattatag aatatagaat tatttatgtc
    47821 aatgaataaa gtttaatcaa aattcaataa gtaaggctat taatttatta taaaattatg
    47881 gtacattgac aaaacaagta aaataattta tattcaaatt ggtaataacc actttggata
    47941 tattatatga ggtatttttt attttacaga ttaatttatt gtttagaaaa gtaaaaatta
    48001 ataatgagga gaatctagta tgattacagt aataacattc aacccatcta tagatagaat
    48061 gtatagagtg aataatataa acataggtga ggtgcaaagg gtagtaagta agaatgcaac
    48121 tgctggaggt aaaggattaa acgtagctaa ggtatgtaaa attttaaaag aaaatccttt
    48181 agctatggga tttctaggcg gatttaatgg agaatttata aaaaatgaac ttagaaaact
    48241 agatatacaa aataaattta caaaaataga gcaggaaact agaaattgct taaacataat
    48301 tgaggacaat aaagttagta cagaattttt agaaaaagga ccaatagtag aaaaaagcga
    48361 tttagagaga tttgaaaatg atatagagga agtactaaaa aatactaaaa tactggtagc
    48421 ttctggtagt tattgtgaga atatgccgat agactattat gaaaagatag gaaatacatg
    48481 tagagcaaat aatataaagt ttatattaga tacatcagga gaagcattaa aaatagcttt
    48541 aaaatcaaaa ccatatttaa taaaaccaaa tatagatgaa ataagacatt tattaggaat
    48601 gaatatagaa tcaagagagg aaattatctt gtcaggaaaa aaacttattg aaatgggtgc
    48661 agagaatgta tgtatatctt taggaaaaga tggtatggtg tatttaaacg aagataatgt
    48721 atatgatgta aaagtaccag ttgtaaagtg tataaatact gtaggtagtg gagatagcac
    48781 agtggctgga tttagtgtag gtattttaag gggttataaa atagaaaatt tacttaaatt
    48841 tgcaaatgct tgtggtataa gtaatgcttt aaatatggaa actggatttg tagatttggg
    48901 agaaattgat aaatatcaag atttagttga agtaaaaaag ttaagttaaa tacatttaat
    48961 gggaatataa taaatttaat gttgttgtaa tagattttaa ttttaatata aaagatatag
    49021 tcttatataa aaataggtta gaaaaattca tttttaacct atttttataa taaatatcta
    49081 ttttactgaa tttataattt tctctagtaa tctttgagct ttgatacact cttctccatt
    49141 aatagctggt tttatattat tttctataca atcaataaaa tgatttgata tatcctcaaa
    49201 acccttttgt tttaatatat ttacccatgc accactttgc tttaaagtta ggtttccacc
    49261 ttcttctatt tccagtacat taagattctt aactctttga atcttaccct ttgaaagaat
    49321 ttcaacttgt tctaactttg ttccagaatc tctatgcata gaggtgttta tagagaaatt
    49381 tttaccttta agtttatgac ttacaaatat aagattctta ttgtctgtta aaaataaatc
    49441 ctctccacta atttcaacat cttcatttgc taaccataaa gcagtgtcaa taacatgtat
    49501 atagtcatct ataagagtac tgtcgaatct aacatttctt aaggaattta atccatgttt
    49561 acatatattg atagatacaa tttctgtagc attattttta atttctttgt acataggaca
    49621 gaatctacgg ttgaatccaa ccattaaatt taagtttttt ttcgtactta actcgataag
    49681 ttcttctcct tgacttactg ttgaagcaag aggtttgtct acatatacat gaacacctaa
    49741 atttaataat atttttataa tttcgtagtg tgtttctgta gagctatgta gaaaaataca
    49801 atcacatttt ttagctaggg attcaataga atcgaatggc atgattctat aatcactaca
    49861 gactttttcc cttttaactt tatttggagt aaaagctcct acaaactcaa atctctcaga
    49921 cttagttaat ataggtaaat atgccttttg agctatggag ccaagaccta tcattcccat
    49981 ttttatattt ttcataatac tttctcctaa ggtttttata tttacagaat tgattataat
    50041 tcagacacat aaatttgtaa actttgtatc aaaaaataaa tttaaagctt atataagtaa
    50101 attagcgaaa taagaaatat tgataaagta attttgtagg atatccataa aattgattga
    50161 tataaatatt ttatgtattg acaaaaaatt caaattaatt tatatttgga ttagtggtaa
    50221 ttaccacttt ggtctgaaca agcttttatc gtaaaataaa aatactaaag tatatagagt
    50281 atgattttat taaattttta aagataaact ataaaattaa ttaaaatcta tagtgtatat
    50341 tttatagtga gtttaagtag tactaaaaaa tctaagcaaa aaattataaa agagtatatt
    50401 tttttatagt ttaaagtggt tataacctca ttaccaaatg taaacgttac aagtatgaaa
    50461 ggagaatgaa aatgtttaaa ttagaagaac aaaaactaaa agatttaggt gcaataataa
    50521 caactaatga aataaaacaa cagcctgaat tatggttaga aacttatgaa atatataaat
    50581 ctaacaaaga aaaattaagt agattcatag atacaataag taataatcat gggcagttta
    50641 gagttatatt tacaggtgct ggaacttcag cttacatagg aaattcaata cttccttacc
    50701 ttaaaaataa aaatgacata agaaaataca tatttgaagc aatacctact acggatatag
    50761 tttcaaaccc atatgattac ttaaaaaaag atataccaac attattaatt tcatttgcaa
    50821 gaagtggaaa tagtcctgaa agtttagcag ctttaaattt agggaataag attgtagata
    50881 atttttatca tttagctata acttgtaatc cagaaggaga actggctaag atgactaaaa
    50941 acgacgaaaa taattacttg ttattaatgc catcaaaatc aaatgatgaa ggctttgcca
    51001 tgacaggaag tttttcgtgt atgatgttat ctgctatgtt aatattcgat agtttagaag
    51061 atgatgtaga aaagtcatat ataaatgcaa taattgaaat gggaagaaat gttatagata
    51121 gaaaagatga aatacatgag ttaataaata aagattttga tagagttgta tatttaggtt
    51181 caggaggatt aggtggtttg acacaagaag cacaactaaa acttttagaa cttacagcag
    51241 gaaaaatatc tacagtgtat gattctccta tggggtttag acacggacca aaatcattta
    51301 ttgatgaaaa tacacttgta tttgaatttg tatctaactg tttatataca agaaaatatg
    51361 atttagatgt tttagaggag ataaaaaggg ataaaattgc taaattcact tgtgcagtgt
    51421 cagtagaaaa tgaaaataac ttctcaggaa caaagtttga gtttaaagaa aagtataata
    51481 aattaccaga tgtgtatctt gcaatgccat acatattgtt tgcacaaact atagcattgt
    51541 ttgtatcagt aaaggttgga aataagccag atactccatc agctacagga acagtaaatc
    51601 gagtagtaaa aggtgtaaca atatatgaat attaaaagat aagattaaaa tattataact
    51661 aaataatttg ggagggtatc aatgtggcat tagtaacaac aacatcaatg cttaaaaagg
    51721 cacaagatga agggtatgca gtaggagcat ttaatgtaga aaatatggaa atggtaattg
    51781 ctgttataga agctgctgag gagttaaatt ctccagtaat attacaaact actccatcta
    51841 ctgtaaaata tgcaggtctt gattattatc tagcaaatgc aaaggttgct gcagaaagag
    51901 taaatatacc tgtagcaatg catctggacc atggttcaag ttttgacctt gcaatgaaag
    51961 ctttaagaag tggatataca tcaataatga tagatggttc tcatgaaagc tttgaaaata
    52021 atatatctat atctaaatca gtagtaaaag catgtagtcc ttctcttata ccagtggaag
    52081 cagaacttgg gaaagttggt ggaaaagaag atgatttaga tggcgggaat agtaatgatt
    52141 atacaaatcc aaaagaggca aaagagtttg tcgatagaac aggaatatct tcattggcta
    52201 ttgctatagg tactgcacat ggattgtata agggtgaacc taaaatagat ttagataggt
    52261 tatcagaaat taaaaatgta gtttcaatac ctttagtatt gcatggaggt tcaggaatac
    52321 atgatgatat tataaaagaa gctataaaaa gaggtattgc aaaagttaac tatgcaactg
    52381 aattaagaat tgcatatagt aaaggggtta aaaaagtttt agatgaagat tctgaggtta
    52441 tagaccctaa aaagtatgga ttagcagggc ttcaatcagt aaaggatttt gtgaaagaaa
    52501 agattaaagt gtgtgggtct gtaaatagag tataaaaata aaatatccta aatttatttt
    52561 agataagttt aggatattta ttatatgaaa aatttatatt aattatatgt aaatggtgta
    52621 tttttttcat ttaattgatg aaaaatatca gcttttattg gtaaagtatt acatatataa
    52681 ttaaaatata ctaaaaattg aactatatat tttattatta taaaatattt atattaaatt
    52741 gtggaattaa aatatactag gatataaaat tcttaaataa aaagaaaagg ttgtttttat
    52801 aaaaaatatt ttttatacca ctttaaaaaa cacaataata acactgaggg aggtggggat
    52861 atgagagaac gcattattct tgaaacatta aaaaatagta gtggttatct aaacatagat
    52921 tattttgcag ataagttggg ggtatctact cgaactatca gaaatgaaat aaaaaagata
    52981 gaaactataa aagaatgtaa tggttttaaa ttagaatata agtctaaatt aggatatatt
    53041 ctaaatatta aagaccaaga taaattcgag cattatctaa gaaacttacc ctcagattta
    53101 gttgaaaatc cagaacaacg gttggagtct ataatagtag agcttttagt aaatgaaggt
    53161 tataaaacta tagaacagtt aagtaaaaaa tttctagtaa gttcatccca aataaaaaat
    53221 gatttaaaaa agatagatga aaagataaag gatacagaat taaaactaga gcgaaaggct
    53281 cattatggaa ttaaaataga aggttctata aaagaaattc aaagtatatt ggtggatagt
    53341 tattttagag ggaatagaaa tataacagag tacaggaata agtttataga taatttaaaa
    53401 ttagctaata ttagaagtac aataaaaaat gtacttaatg aacatgattt agaagcaaat
    53461 ttaacagaac ttgaggaaat attagcacaa attattatat tgtatataag a
//
LOCUS       DAGSHT010000007        50438 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGSHT010000007 DAGSHT010000000
VERSION     DAGSHT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.008"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1011)
                     /locus_tag="MJB72_000367"
     CDS             complement(<1..1011)
                     /locus_tag="MJB72_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBY8261545.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLI"
     gene            1474..2670
                     /locus_tag="MJB72_000368"
     CDS             1474..2670
                     /locus_tag="MJB72_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBY8261546.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            2763..3287
                     /locus_tag="MJB72_000369"
     CDS             2763..3287
                     /locus_tag="MJB72_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBY8261547.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            3312..3488
                     /gene="rpmF"
                     /locus_tag="MJB72_000370"
     CDS             3312..3488
                     /gene="rpmF"
                     /locus_tag="MJB72_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBY8261548.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            3695..4252
                     /gene="fapR"
                     /locus_tag="MJB72_000371"
     CDS             3695..4252
                     /gene="fapR"
                     /locus_tag="MJB72_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBY8261549.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            4263..5285
                     /gene="plsX"
                     /locus_tag="MJB72_000372"
     CDS             4263..5285
                     /gene="plsX"
                     /locus_tag="MJB72_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBY8261550.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            5311..6297
                     /locus_tag="MJB72_000373"
     CDS             5311..6297
                     /locus_tag="MJB72_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBY8261551.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            6406..7335
                     /gene="fabK"
                     /locus_tag="MJB72_000374"
     CDS             6406..7335
                     /gene="fabK"
                     /locus_tag="MJB72_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBY8261552.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            7351..8301
                     /gene="fabD"
                     /locus_tag="MJB72_000375"
     CDS             7351..8301
                     /gene="fabD"
                     /locus_tag="MJB72_000375"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBY8261553.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGHGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            8301..9050
                     /gene="fabG"
                     /locus_tag="MJB72_000376"
     CDS             8301..9050
                     /gene="fabG"
                     /locus_tag="MJB72_000376"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBY8261554.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            9104..9328
                     /gene="acpP"
                     /locus_tag="MJB72_000377"
     CDS             9104..9328
                     /gene="acpP"
                     /locus_tag="MJB72_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBY8261555.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            9357..10595
                     /gene="fabF"
                     /locus_tag="MJB72_000378"
     CDS             9357..10595
                     /gene="fabF"
                     /locus_tag="MJB72_000378"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBY8261556.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            10821..12539
                     /locus_tag="MJB72_000379"
     CDS             10821..12539
                     /locus_tag="MJB72_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8261557.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            12570..14570
                     /locus_tag="MJB72_000380"
     CDS             12570..14570
                     /locus_tag="MJB72_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261558.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            14921..15166
                     /locus_tag="MJB72_000381"
     CDS             14921..15166
                     /locus_tag="MJB72_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBY8261559.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            15201..15926
                     /locus_tag="MJB72_000382"
     CDS             15201..15926
                     /locus_tag="MJB72_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBY8261560.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            15948..17261
                     /locus_tag="MJB72_000383"
     CDS             15948..17261
                     /locus_tag="MJB72_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY8261561.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            17467..18006
                     /locus_tag="MJB72_000384"
     CDS             17467..18006
                     /locus_tag="MJB72_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8261562.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            18095..20080
                     /locus_tag="MJB72_000385"
     CDS             18095..20080
                     /locus_tag="MJB72_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBY8261563.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            20304..21272
                     /gene="spoIIIAA"
                     /locus_tag="MJB72_000386"
     CDS             20304..21272
                     /gene="spoIIIAA"
                     /locus_tag="MJB72_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBY8261564.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            21262..21783
                     /locus_tag="MJB72_000387"
     CDS             21262..21783
                     /locus_tag="MJB72_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBY8261565.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            21800..21997
                     /gene="spoIIIAC"
                     /locus_tag="MJB72_000388"
     CDS             21800..21997
                     /gene="spoIIIAC"
                     /locus_tag="MJB72_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBY8261566.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            22013..22399
                     /gene="spoIIIAD"
                     /locus_tag="MJB72_000389"
     CDS             22013..22399
                     /gene="spoIIIAD"
                     /locus_tag="MJB72_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBY8261567.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            22484..23689
                     /gene="spoIIIAE"
                     /locus_tag="MJB72_000390"
     CDS             22484..23689
                     /gene="spoIIIAE"
                     /locus_tag="MJB72_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBY8261568.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            23749..24366
                     /locus_tag="MJB72_000391"
     CDS             23749..24366
                     /locus_tag="MJB72_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBY8261569.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            24378..24932
                     /locus_tag="MJB72_000392"
     CDS             24378..24932
                     /locus_tag="MJB72_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBY8261570.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            24959..25648
                     /locus_tag="MJB72_000393"
     CDS             24959..25648
                     /locus_tag="MJB72_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBY8261571.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            25758..26120
                     /locus_tag="MJB72_000394"
     CDS             25758..26120
                     /locus_tag="MJB72_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBY8261572.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            26205..26711
                     /gene="nusB"
                     /locus_tag="MJB72_000395"
     CDS             26205..26711
                     /gene="nusB"
                     /locus_tag="MJB72_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBY8261573.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            26695..27765
                     /locus_tag="MJB72_000396"
     CDS             26695..27765
                     /locus_tag="MJB72_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBY8261574.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            27762..28967
                     /locus_tag="MJB72_000397"
     CDS             27762..28967
                     /locus_tag="MJB72_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBY8261575.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            28969..29184
                     /gene="xseB"
                     /locus_tag="MJB72_000398"
     CDS             28969..29184
                     /gene="xseB"
                     /locus_tag="MJB72_000398"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBY8261576.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            29187..30074
                     /locus_tag="MJB72_000399"
     CDS             29187..30074
                     /locus_tag="MJB72_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBY8261577.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            30104..30541
                     /locus_tag="MJB72_000400"
     CDS             30104..30541
                     /locus_tag="MJB72_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBY8261578.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            30575..32440
                     /locus_tag="MJB72_000401"
     CDS             30575..32440
                     /locus_tag="MJB72_000401"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBY8261579.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            32573..33385
                     /locus_tag="MJB72_000402"
     CDS             32573..33385
                     /locus_tag="MJB72_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBY8261580.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            33405..35084
                     /gene="recN"
                     /locus_tag="MJB72_000403"
     CDS             33405..35084
                     /gene="recN"
                     /locus_tag="MJB72_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBY8261581.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            35298..36164
                     /locus_tag="MJB72_000404"
     CDS             35298..36164
                     /locus_tag="MJB72_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261582.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(36257..36748)
                     /locus_tag="MJB72_000405"
     CDS             complement(36257..36748)
                     /locus_tag="MJB72_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8261583.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(36809..37249)
                     /locus_tag="MJB72_000406"
     CDS             complement(36809..37249)
                     /locus_tag="MJB72_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8261584.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            37410..38468
                     /locus_tag="MJB72_000407"
     CDS             37410..38468
                     /locus_tag="MJB72_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBY8261585.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            38610..39434
                     /gene="spo0A"
                     /locus_tag="MJB72_000408"
     CDS             38610..39434
                     /gene="spo0A"
                     /locus_tag="MJB72_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBY8261586.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            39847..40968
                     /locus_tag="MJB72_000409"
     CDS             39847..40968
                     /locus_tag="MJB72_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261587.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            40986..41861
                     /locus_tag="MJB72_000410"
     CDS             40986..41861
                     /locus_tag="MJB72_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBY8261588.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSAKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            41885..42577
                     /locus_tag="MJB72_000411"
     CDS             41885..42577
                     /locus_tag="MJB72_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY8261589.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            42585..43409
                     /locus_tag="MJB72_000412"
     CDS             42585..43409
                     /locus_tag="MJB72_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY8261590.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            43414..44481
                     /locus_tag="MJB72_000413"
     CDS             43414..44481
                     /locus_tag="MJB72_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBY8261591.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            44483..45019
                     /locus_tag="MJB72_000414"
     CDS             44483..45019
                     /locus_tag="MJB72_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY8261592.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            45087..45692
                     /locus_tag="MJB72_000415"
     CDS             45087..45692
                     /locus_tag="MJB72_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBY8261593.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            45704..46618
                     /locus_tag="MJB72_000416"
     CDS             45704..46618
                     /locus_tag="MJB72_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY8261594.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            46660..47832
                     /locus_tag="MJB72_000417"
     CDS             46660..47832
                     /locus_tag="MJB72_000417"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBY8261595.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            47879..48691
                     /locus_tag="MJB72_000418"
     CDS             47879..48691
                     /locus_tag="MJB72_000418"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBY8261596.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            48895..50220
                     /locus_tag="MJB72_000419"
     CDS             48895..50220
                     /locus_tag="MJB72_000419"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBY8261597.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        20353 a         5093 c         9367 g        15625 t
ORIGIN      
        1 tataagttgc acgtcgcttt ttttaatacc taacaatata ttgtttagag ttcgtttgat
       61 tttggttaaa gtgtatcgct tggatttaat tgctaattgt agttcatgta gaaattcaga
      121 tgtaaagata tctttataga ttctgttttc tatgccttca tttacttcaa aatactcttt
      181 taaagtgtcc atatctctaa gtataattgt ttttaaaata tcaaagaaga agtcatcaaa
      241 cataggtaaa aaaccattat ctattttttc atttaggatg ttataagttt tttctggaat
      301 aacatttttt aaatgaaaaa ggtctgtatt ttctttaagt gaagttctta tagcagtagc
      361 agaacaaata tcactctcaa tttcttcgga attatattct gattggaccc tggagatagt
      421 aaatggtttt atactactgt ttaaatggat gaggtttttt atgtattcta tacccagtat
      481 gttgtttgat gaatttaatg tttttgataa ttgttccttt gaataatttt gtataaaatt
      541 attttttttg tcttgatgta tataatcaaa aagtgcatta gctctagctg ttggaaataa
      601 cagaccatca tctaaatatt tttttaaaag taacttaaat tcatttggtt catttgctaa
      661 aatttcagct agattataaa gaatttctgt atttccttct tcactaccaa aacatataga
      721 atttatgcag tttagtgaat ttaaaattgt tatagcacca tgagaaaaaa tttcagcact
      781 ttgacaagca aataaagttg gtaattctat aactaagtct acaccatttt cgacagcaat
      841 ttttgctcta gtgtatttat caaataaagc tggttcgcct ctttgtagaa aatttccact
      901 cattattgct acagtatgtg ttgcatttgt tttttctata gatttagata aatgatatat
      961 gtgtccatta tgaaatgggt tatattcaac tattaatcca agtatgttca taaaatactc
     1021 cttttatctt taattatttt atatatatca caatttatta taatatgtat aaaatttaaa
     1081 tttttaatta taaaattaaa aaacgtaaaa aaatttataa aaatgtagaa atgttaccta
     1141 cataaaaaac tggttaaatg ttattaaaat aactttatta gctctttata ttatattatt
     1201 ataacaaatg ggaaagaata atctacatta agttaacttg ttaatatatt attagtgaaa
     1261 tacacaattt accatactag aaatatgata taatataaaa aaaaagtata tacttttata
     1321 ttaaaaggtg atatattatt tatgaaaatc gtttaatgta tacaataata tatctataac
     1381 aaaataataa aagatagagt tttatttaga catttattac aaatcaaaat ttattacata
     1441 ttatttataa aaaaatctaa ggaggaaata ataatgaaaa tattagtatt aaactgtggg
     1501 agttcttcat taaaatatca attaatagat atgaataatg aagaagtttt atgtatagga
     1561 ttggttgaaa gaataggaat agaaggttct atattaaaac atgaaaaagc aggtagagat
     1621 gataaatacg ttgttgaaca accaatgaag gaccacaaag atgcaatagc attagtttta
     1681 gaagctgttg ctcatccaga atttggtgca gttaaagaaa tgaaagaaat agatgcagta
     1741 ggacataggg ttgtacatgc tggagaaaaa tttgcaactt cagtagtaat aactccagaa
     1801 gtagaagaag ctttaaaaga atgtatagat ttagctccac ttcataatcc agcaaacata
     1861 atgggaatag atgcttgtaa agctatactt ccagatgtac caatggtagg tgtatttgat
     1921 actgctttcc atcaaacaat gcctaaatct tcatacttat atggtttacc tcatgagcta
     1981 tatactaaat atggggtaag aagatatgga ttccacggaa cttctcataa ttatgtatct
     2041 caaagagcag cagagatatt aggaaaagat ataaaagatt taaaaatagt aacttgtcac
     2101 ttaggaaatg gtgcttctat agctgctgtt gatggtggaa aatgtgtaga tacatctatg
     2161 ggattcactc cattagaagg attaataatg ggaactagat gtggagatat agacccagct
     2221 atattaccat tcttaatgag aaaagaaggt ttagatgctg atggattgga taaattaatg
     2281 aataaagaat ctggagtata tggaatgact ggaatttcta gtgacttcag agatatagaa
     2341 gatgcagcta aaaatggaga cgaaagagct caagctacat tagaagcgta tgttaaaaaa
     2401 gtacaaaaat atataggcgc ttatgctgct gaaatgaatg gattggatgt tgtagtattt
     2461 actgcaggcg ttggtgaaaa tggaaaggct ataagagcag atattgcttc taacatggag
     2521 tttttaggaa tgaaattaga taaagaagct aatgatgtta gaggaaaaga aacagtaata
     2581 tctactgctg attctaaagt aaaaatgctt ttaataccaa ctaatgaaga attaatgata
     2641 gctagagata cattaagatt ggtaaaataa ttaaattaaa cctatgccta tcaagtaaaa
     2701 atacttgaca aacaaatgct agatttgtat aataaatttg gtgatatgtt gaggtgaact
     2761 taatgaaagt tagcatagaa aaaataaata gaaaagaaac tgacaaaata gacttgaatt
     2821 tttgtgaaaa aattgatact attagttatt gtgatgagat ttacaaatta gtatcacctg
     2881 taaatttaaa agggaaagtg tcaaagacta ataaaggttt gtatctagat atagatgtca
     2941 attttacaat tgttgacaat tgttcaagat gtcttaaaga agtagaaata ccactagagt
     3001 attctataca aggtttttta gtaaaagaag aagattatga tgaagatgag tttgaggaat
     3061 ttgatccttt tatattcgat ggtgaagaaa tcgaccttat tgatataata gaacagacat
     3121 tagattttaa tgtacctcat aaagttcttt gtagtgaaaa ctgtaaaggt ctttgtcaag
     3181 tatgcggggc aaacttaaat gaagaagagt gttcttgcag tgaaattaca aacgatgagg
     3241 aatacataga tcctcgtttt gctaaattaa aagatttatt taattaaatt ttgactaagg
     3301 aggtggcgta aatggcagta ccaaagcgta aaacgtctaa atcaaacaca aaaatgagaa
     3361 gagcagctaa ctcaaagatg gaagcaacag gatttgtaag ttgcccacaa tgtcatgagc
     3421 caaaattacc acatagagtg tgtccagact gtggatatta taaaggtaaa gaagttgtgt
     3481 ctaagtaata gcttctaaaa agcatgtaga gtatactcta catgtttttt ttgtacatat
     3541 aaaaaaataa gataatttac tagagaaaat aagtggattt tatgcatatt aaaaaataga
     3601 aaaataataa tataagtatt gaaaattttt gtgtgttact atatactact attagtagct
     3661 ggtactaatt acagctataa aatttggagg gaacatgaaa aagaaaagta aagcccaaag
     3721 acaaaaagag cttatagata tgctaaagac agatcctttt tatactgatg aagaattatc
     3781 aagtctgttt gatgtaagta tacagaccat aagattagat agaatgagtc taaacatacc
     3841 agagctaaga gaaagagtca aatcaatagc agaaactcag agttcaaaag taaagacttt
     3901 aggagtaaag gaaattacag gagaaatcat tgatttatct gtagggcgat taggtatatc
     3961 tatgttagaa gttacacagg acatgattta ctctaagact aatactttaa aagatacgta
     4021 tattttttct ctagcagatt cattggcaat ggctataata gatgcaccta aggttattat
     4081 gagagttgca aatgtaaaaa gttttaagtt gatagaacaa caagataggc ttatagcaaa
     4141 agcagaagtg tatagaaata tagataaaaa gcattatgta aaagttgtta tcaacaataa
     4201 agcccaagag caaatattta gaggaaagtt tatctttgaa gaattagatt aggagggaaa
     4261 ttatgaaaat agtaattgat ggaatgggtg gagataatgc acctaaatct aatgtagaag
     4321 gcgcagtaaa tgctataaaa gaatatcagg ttgatttaat aatcacagga gataaagatt
     4381 tacttgaaaa ggaattttca aattatgaat ttgatagaaa taaattagag atagttcata
     4441 ctactgagat tatagaaaac gaagataaac cagtaaaggc tataagaagt aaaaaagatt
     4501 catctatggt agtagcatta aatcttgtaa aagaaggaaa ggcagatgct ataatatcag
     4561 ctggaaatac aggagcctta cttgctggtg gattatttgt tgttggtaga ataaaaggaa
     4621 tcgatagacc ttgcttatgt tcagcaatac caaatgtaaa gcgaggaatg acacttatag
     4681 cagactgtgg cgcaaatgca gattgtaaac ctaagaatct agttgaattt gcagctatga
     4741 gtaatattta ttcaagaaaa gttttaggtc ttgaaaatcc taaggtagca ttagcaaatg
     4801 ttggactaga agaaggcaaa ggcaatgatt tagtcaaaag gtcatatgaa gaaattaaaa
     4861 aattagattt aaattttata gggaatgtag aagctagaga agttataaat gcatacacag
     4921 atataatcat atgtgatgga tttacaggta atatattact taaatctgct gaaggtgtag
     4981 cactttcagt tatgagtctt ataaaggaaa cttttatggc cagtacaaaa agtaaaatag
     5041 gagcattact tataaaggat gatttaagaa aattaaaaag ttttatagat tattctgagt
     5101 atgggggagc tccactttta ggtctaaatg gaggagttat aaaagctcat ggtagttctg
     5161 atgctaaagc tataaaaaat gctataaatc aaggaataaa attttcaaag ggaaaagtcg
     5221 ttgaagatat taaccaattt ataagcaaat acaatgaaga aaataaaaat aacgaagatg
     5281 aataaactcg ttttggaggt ttaatatata atgaatacaa aggctggaat attaggagta
     5341 ggaagttatc tacctgagca atcatatgat aattttcact tcgaaaaaat aatggataca
     5401 tcagatgagt ggataagtac tagaactggt attaaggaaa gaagatttgc caaagagtct
     5461 gaagcaactt ctgacttagc atctaaagct gccttaaaag ctatagaatg tgcaaagtta
     5521 aacgtagagg atatagagct gattatatta gctacaatta ctcctgatat gtctttgcct
     5581 tcaactgcat gtatagtaca agatgcaata ggagcagtga atgctactgc atttgatata
     5641 tcagcagctt gttctggatt tgtatatgga gtaactattg caaagcaatt tgtggaaaca
     5701 ggttgctata aaaatgtgct tgtaatagga gcagaaactt gttctaagtt tttaaattat
     5761 gatgatagaa caacagcagt attgtttgga gatggagctg gagcagctgt tattggccct
     5821 gtaaatgagg gtggaatact gtctacacat atgggctctg atggtaaagg aaaagattgt
     5881 ttaaaagtac ctgctggagg ttcaagactt aaggcaagta aagagactgt agaagctaat
     5941 ctacatacaa tagaaatggc aggtagtgat gtgtttaaat ttgcagttag aaagatggca
     6001 gaaacttctt taagagctct tgaaaaagca aatctaaata caactgatat agactactta
     6061 gtgccacatc aagctaatat aagaatcata caagcttctt ctaagagatt agagcttgat
     6121 atgaaaaaag tttatgtaaa tatagacaaa tatggaaata tgtctgctgc atctatacca
     6181 gtagcattag atgaagctta tagagaaggt aaaattaaaa agggtgataa tgtagtttta
     6241 gttggctttg gtggaggtct aacttggggg gcttcagttg tcaagtggac tttatagtat
     6301 aaaatatcta aaatgattta attaacaaaa ttaaacttat acacaaaata aagttgatta
     6361 ataataaaaa gtttactttt atgatttcaa gtgaaggaga attgtatgaa taaaatttgc
     6421 aaaatattaa atataaaata cccagttatc caagggggaa tggcatgggt agctactgca
     6481 tcattagcaa gtgctgtatc taatgcagga ggacttggca taatagcagc aggaaacgca
     6541 ccaaaagaag ctataaagaa agaaattgtt gagtgtaaaa aattaacaga taaacctttt
     6601 ggagtaaatg taatgcttat gtcgccattt gttgatgata taattgattt gattatagaa
     6661 gaaaaagttc aagttattac tactggtgct ggaaatcctg caaagtatat ggatagatta
     6721 aaggaagctg gaacaaaggt tattcctgta gtacctacaa tagctttggc acaaagaatg
     6781 gaaaagctag gagctacagc agtaatagca gaaggtactg aaggtggagg acatatagga
     6841 gaacttacta ctatggtctt agttccacaa gttgctgatg ctgtaaacat acctgtaata
     6901 gctgctggag gaattgtaga tggtagagga attgcagcat catttgcatt aggtgccagt
     6961 gcagttcaag taggaactag atttatttgt agtgaagagt gttctgtcca ttcaaactat
     7021 aaaaacttag tactaaaagc aaaagataga gatgcaattg taacaggaag aagtactggt
     7081 catccagtaa gaacattaaa aaataaacta tcaaaagaat ttttaaagat ggaacaaaat
     7141 ggagctactc ctgaagaact ggataaaaaa ggtacaggag ctttaagatt tgcaacagta
     7201 gatggagaca tagaaaaagg ttcatttatg gcaggtcaaa gtgctgctat ggtaaaagaa
     7261 ataacacctt gtaaggaaat tatagaggct atggtaaatc aagcaagaga gattatgcca
     7321 gcaatagaac tgtaaatcag gaggagcaag atgggaaaag ttgcactagt attccctggt
     7381 caaggagctc aatatgtagg tatggccaag gatttatatg aaaataatac tgttgctaaa
     7441 agtgtaatag atgaggctag tgatgcctta aatatggatt taaaaaacct tatgtttaat
     7501 ggaaatgaag aagaactttc taaaactgaa aacactcaac ctgctatggt tacacatagt
     7561 gtagcagtat taaaagcggt acaagctcaa atagatttaa aatataatgc atgcttagga
     7621 ttatccttag gagagtatag tgcgttggtt gcagcagatg caattgactt taaagatgct
     7681 gtatgtttag ttaaaaaaag aggtaagttt atgcaggaaa cagttccagc aggtgttgga
     7741 gctatggcag caattttagg tttagataga aatgtacttg aaaatgtggt taaagatata
     7801 caaggaggta ttgtagaagt agcaaattac aattctcctg gacaaatagt tatatctggt
     7861 gaaaatgaaa agatagaaga agcaatggta aaatgcaaag aagcaggagc aaaaagggca
     7921 gttaaattaa atgtaagtgg accttttcac tcatcaatgt taaaagaagc tggtgtaaaa
     7981 ttggccagtg aattagaaaa agttaacata actaaaccta aagttgatgt ggtggcaaat
     8041 gtaaatgcag attactataa aaacgaaggt aaagacctgc ttataaaaca agtaagttca
     8101 tctgttcttt gggaagattc tatagaaaga cttttaaatg atggatatga tacttttata
     8161 gaaatgggac atggaaaaac tttaaaagca tttattaaga agatagcttc caataaaaaa
     8221 gcaaatgtca atatttataa tatagatggt atagattctc ttaatgaatt tgtacaaaat
     8281 tatagaaatg gagagatata atgataaatc ttacaggaca agtagcagtt gttactggtg
     8341 gctctagggg tataggaaaa gaaatagcaa aaaaactagc atcttttgga gctgatgtag
     8401 taatcaatta tacttctaaa gaagatgaag cactaaaaac taaaaatgaa atagaaagta
     8461 tgggggtaaa gtgtacctct ataaaatgtg atgtatctaa atttgatgaa gtgaatcaaa
     8521 tgatagattc tgttgtaagc gaatttggaa aaattgatat attggttaat aatgcaggca
     8581 taactaaaga tggtctgctt atgagaatga aagaagaaga ttttgataga gttatagata
     8641 taaacttaaa aggtgtcttt aattgtacaa aagcagttac taaacctatg atgaaaaaga
     8701 agtatggaag aataataaat atgacttcag tagttggaat tatgggtaat gcagggcaaa
     8761 ctaattattg tgcatcaaaa gcaggtgtaa ttggatttac aaaagcttct gcaagagagt
     8821 tagcatcaag aaacataaat ataaatgcag tagcacctgg atttatagaa acagatatga
     8881 ctaaagtact aagtgatgat gtaaaagaat caacactagc aaacatacca aagaaatctt
     8941 atggtaaacc agaagatgta gccaatgccg tagcattttt agttagtgac atgtcaagtt
     9001 atataacagg acaagtaata aatgtagatg gtggaatggt aatgcaataa taatttaaaa
     9061 agtagagaaa tctattaaaa taaaaaattt taggaggtac acaatgttta ataaagttgt
     9121 agaaataata atagagcaat taggagtaga agataaagaa ataactatgg aaacatcatt
     9181 aatgaaggat ttagaagcag attcattaga tgcagtagaa ataataatgg cacttgaaga
     9241 tgaatttggt atagaaatac cagatacaga agcagaaaac ttcaaatcta taggagatat
     9301 agttaagtat atagaagcta ataaatagtc gagctttaaa aaagtggagg tatccgatga
     9361 ataaaagagt agtaataaca ggacttggat gtgttacgcc gttgggaaca ggaaaagaag
     9421 agttctggag taatataaaa tctggagtat ctggtataga taaaataact aattttgatg
     9481 ctagcacata tcaaactcag atagcaggag aagttaagaa ttttcatcca gaagaatata
     9541 taagtaaaaa agaattgaag agattagata aatttgctca atttgcaata gtatctgcta
     9601 aattagcagt tgaagatgca aatcttgatt tagataaagt tgatagagaa cgttttggtg
     9661 ttataatagg ttctggaata ggtggagttg aagctataga aactcaacat aaaattcttt
     9721 tggaaaaagg aaataaaaga gtaagttcac tttttgtacc tatgatgata ggaaatatgg
     9781 cagctggtca agtttcaata tttttaggag caaaagggcc taacactaat gtatgtacag
     9841 cttgtgcatc aggaactcat tcaataggtg atgcttttaa ggtaattcaa agaggtgatg
     9901 cagacatcat ggtagcaggg ggctctgaag ctgctgtaac aggtcttgct tttgctggat
     9961 tctgtaacat gaaggctatg tcaactagaa atgatgaccc taaaactgca tcaagaccat
    10021 ttgataaaga cagagatggt tttgtaatgg gagaaggagc aggtattgtt atcttagaag
    10081 acttagaaca tgctttagca agaggcgcta aaatatatgc agaagtagtt ggatatggtt
    10141 taactgctga tgcataccat atgacaactc cagctgaaaa tggagaggga gcagctaggt
    10201 ctatgaatat ggctttgaaa gatggaaatg ttccattaga agaagtagat tatataaatg
    10261 ctcatggaac ttcaacttat tacaatgatt tatatgaaac tatggctata aaaactgtat
    10321 ttggagaaaa ggcatatgac ttatgtgtgt catcaacaaa atctatgact ggtcacttat
    10381 taggagcatc aggtgctata gaggctgttg tttgtgctat gagtatagaa gatagttttg
    10441 tgcctccaac tataaacata caagaagttg gtgaagattt ggatttagat tatgttccaa
    10501 atcaaggtaa agaaaaaaat attagatatg cactatcaaa ttctttagga tttggtgggc
    10561 ataatgctac aatagtacta aaaaaatatg tataaataaa tattaaatat aaaactaaaa
    10621 aacaagcact tgttttaact gtataaattt atattagctt aaatttaaag tctcctaaat
    10681 tattttagga gactttttta ttaagattta agtctagcat atgttgtaaa tcattttgat
    10741 atattgtatt attaattatt aagaggttat ttatatatgc aagttttcat tctacaatat
    10801 acaaaaggag gactaaaatt ttgaatttta gactaccaaa gaaaaggtta cccaaaaaga
    10861 taatttttat atgtataata cttattagta ttattttgtt tataaagtat gaccaaaatg
    10921 gattgaaaga aagagaaatt ataaataagg gaaaatatat gtctgaaagc cttgtggcag
    10981 ataaggagta tgatgaagca aaaaaagtgg ttaagacttc tttcgaatct attccagaaa
    11041 aaaaatacaa taaagtatat aatgaagttt gggatatgtt aaagactatt tcatatgttc
    11101 cagatggaac gaagatagtc attgattctt tagaaaagat gagagaatca gatgtgaaat
    11161 tatctgatga atgtagattt aatatagaaa aaagattagc atcagtgtat ataatggata
    11221 ataattattc taaagcagta gatttaactg taaagtctat aaagttggct gagaaattgg
    11281 ggaataatta tgaaaaagca agactagatg ttgatttggc gagcatattt ttaaaaatag
    11341 gtagctatga aacaggagag aaattaataa aggattcttt taaaatagat attgatgaag
    11401 gtgaaaaaaa tggaatggtt agattatatg ctattataaa tctagcagaa atatatgtag
    11461 aagagggaga atatgataaa gctattgaaa taagtagcag gataaaagat tataaaaagt
    11521 ttgtaaatac ggataattac aatgattttg atataatggc ctctataatg cagtcaaatg
    11581 cgtatgtggg caaaaataat atacaaaagg caaagacttt tttagaaaaa gctgattatc
    11641 ttataaaatc agatagaaca gtttatatat tagataaaga tatgtactat tatatggcaa
    11701 tgggaaactt agaatataaa agcgaaaatt ataattctgc aatagataat tataaaaaaa
    11761 gtttagagat atctactaaa agaaagttaa cccaggagaa aataaaaaat ttaaataaga
    11821 tattaaatat atatgaaaaa caaggaaata tagaatcttt aaatgagtat caaaagatgt
    11881 taataaatga atataaagag aacaaaagta taagagattc agagactagt ttctatataa
    11941 ttgataaagt atcaaacgaa agtaagctat ttgaaaaaac taaaaaagaa ataaaatatt
    12001 ataaaatatt atttacagta attttaattg ctatattagg gtctactttt ttatacaacc
    12061 gattgaagta ttttaaagca caaaatttac atgatggtct tacaaatgtt tataatagaa
    12121 aaagttttga tattatgtat gaaaagtaca gagagaaaga tgataatttt gcacttgtga
    12181 tgatagatat tgataatttc aagctcataa atgataatta tggacataaa tttggtgaca
    12241 ctgttataaa gggaataaca aataccattt gtgttatgtt agaacaagat gataaagtct
    12301 ttaggtacgg aggagaagaa ttttctgtat taataagaaa taaatctggt gaggaagtag
    12361 cagatataat tgacagtata agggttgcaa ttgcaaataa aaaatggaat gatgatgtga
    12421 ctgtgactat aagtgcaggt gtagctcata tttcagatag caaagatgta ctagaagaag
    12481 ctgataaaaa tttatataaa gcaaaacaac ttggtagaaa taaagtagta tataagtaat
    12541 tatatactat ataaataaga ggtataaata tgaaacaatc aatagctata gtaactgata
    12601 aaataactaa actatcatct ttcttagaag aaaatatacg tttggtttta ggtaattata
    12661 ttgatataaa ccactattat ctggaagttt taaaagagaa tgataaaata caaggtgatt
    12721 ttgtactagt aatgaatgat gatagattaa acagtatgag aaagcattta gtacaagata
    12781 gtaagattat agtgataagg agaacactta aagaaaatga aatatatgga ttattttcaa
    12841 taccaaatgg aactgaagta ttggttgtaa atgatacaaa agaaactacc ttagaaacga
    12901 taagcttatt ttacaaaata ggagtaaaaa atttaaagtt tactccatat attgaaggaa
    12961 aaatttataa aaatatagac atagctataa cgcctggagt aaagggaaaa gtaccaagtt
    13021 atataaataa agtaatcgac cttggaaata gatatattga tatatctacc tttatagaaa
    13081 taataaatgg tcttagaata gatgttaaag aaatacgtaa aaatttaatg gcatattctg
    13141 agagtttaat aagtttagat aatggagtta aagataatta taggcaactg tttttaaaaa
    13201 tagaagaaca agatgttata ttgaatctat caaaagatgg aataatattt acttcagcag
    13261 atggtataat aaacacattt aataaagaag ctttaaaaat tttaggtata aatgaaaatc
    13321 cacaaggcaa aaacatagaa gatatcattt ctgaagattt aaatatttta cttgaagaca
    13381 aagaaataat tgatgaagtg gtatttacaa ataaaaaata tataaatgta aataaaaaaa
    13441 gtattttttc tatggggaat aaagctggga tatattataa cttacaagaa ataacgtata
    13501 taaaaaaatt agaacaaaat cttactaata aattaagaga acaaggtcag atagcaagat
    13561 atacatttaa agatataaaa actaaaaata aaaatatggt aaagtgtgta gaattggcta
    13621 aaaaaatatc taaatcagac ctttctgtat taataatagg agaaagtggt actggtaaag
    13681 aattattatc acaatcaatt cataatgctt ccaatagaag taatcaaccg tttatagcag
    13741 ttaattgtgc agcagttcca gatagtctat tagaaagtca actgtttgga tatgaaaaag
    13801 gctcttttac aggagcttta agagaaggaa aaaagggtct atttgaactt gctaataatg
    13861 gaactatatt tctggatgaa attggagata tgcctccatt gctacagaca aagttactaa
    13921 gggtattaca agaaaagcag gttatgccta taggttccca taatgttata aacatagatg
    13981 ttagggtcat agcagcaaca aacaaaaact tactaaagat gattagagaa ggaaaattta
    14041 gagaggacct gtattataga ctaaatgtat tgccaataga tgtaccatca ttgaggaata
    14101 gaaaagaaga tataataaca cttatgaatt tctttttgaa aaacaatata agactttcgc
    14161 cagaagttat aactatttta gaaaaatata attggccagg aaatataaga gagttacaaa
    14221 atgttgcttc ttatgtaagt ctaatgtgtg atagtatggt ttttagagat gacttaccac
    14281 catatataag ggtagaatgt gctaacaatt atgaagatgg atttggttat acaagaaaag
    14341 aatttgagga aatactgagg attctatatg aaagaagaga gtcatcctct ggaattggaa
    14401 gagggtttat aatcgaaaag cttatacagg aaggtattga gatttctgaa agcaaagtta
    14461 gaaaggtgct atcttattta agtgaatgtg gatacatatc atctaaatct ggtagaggag
    14521 gaagtcaaat aacctcagag gggataaaac tttataataa tataaattaa gctaagtatt
    14581 tgatattgaa atcacatatt ttgaatttag atatttaaat aataataaac ttttatattt
    14641 atagagatta atttatatta tgtcagaaag aatattgtaa tttataatta aatattaata
    14701 ggtttttaaa ataggattag ataggtaata ggttaagtta gaataaccca ttacctattt
    14761 ttatttgtaa gaaaagtgta catagaatta gaagtaaaaa atacaactaa taaaactatt
    14821 gaaaatacta gataaataat aaataaaaat aattatagat tttggcatac atattgctaa
    14881 ttatagagat aagacgacaa aataattggg aggtttaatg atgaaataca aaacaacagg
    14941 gacttgtgct acagaaattg aatttgaagt aaaagaaaat aaagttacaa atgtaaactt
    15001 tataggtgga tgtgatggaa atttaaaagg gctaaaagta ttagttgaag gcatgaatat
    15061 agaagatgta atacaaaagt taaaaggaat agagtgtaaa acaaagccaa catcttgccc
    15121 agaccaactg tctctagcat tggaaaatta tatgaatata aagtaataca taaaacaatc
    15181 gattagttag gagtttaatt atgaaaccaa taatagggat tttaggaaat ttaataataa
    15241 tggaaaatgg tatgtttcca ggtcttgaaa ggtcatatgt aaataatgat tatataaatg
    15301 cagtgctaaa aggtggcggt agtccagtaa taattcctgt aaatacagat aaagaagtta
    15361 taaaaaaaca gattgaaatg gttgatggag tcttaatatc tgggggatgg gatatcaatc
    15421 ctcaactgta tggagaagaa acaagagaag aaacgacatt tatatatcca gaagtggatg
    15481 aatttgattt aattgcaata agtatagcat tagaacttaa aaagcctata ttaggtgtat
    15541 gtaggggact acaaatttta aatgtaagtt taggaggaac tttatatcaa gataataatc
    15601 ttatagaagg aagttatata aaacatacac aatcttctaa aagacatgta gctactcata
    15661 aagttgatgt taaagaagga agtattttag agggaatttt aggaaaacaa ttacttacaa
    15721 atagttatca tcatcaaagt gtcaatcagc ttggaaaagg attaaaagcc atagcatact
    15781 caaaagatgg tataatagaa gctattgaga aagaggatga aaattttgta gtaggagtac
    15841 agtggcatcc agaaatgatg gtagattact gtgataaaat ggaaaaacta tttaaatatt
    15901 ttataaatat ttgttcgaat ttataatata ccttgggggg attgaacatg aacgaaaaga
    15961 acaaaatggg attaataagt attatacttt taggtattaa tgcagtagta ggagctggag
    16021 tttttctatt accaggagat gcaatgaaat catttggtgt agcaagtata tttgtatata
    16081 tatttgatat gttgttagtt ttatctatgg cattctgttt tgctgaggtt gcaggtaaat
    16141 tcaataaaaa tggtgctgcc tatgtatata caaaagaagc ttttggagat ttttgtggtt
    16201 ttgaagtagg tcttatgaaa tgggtaatag gatgtatttc ttggggagct ttaatagtag
    16261 gttttcctac atcattatca gcagtatggg ctccagcagg tgaaccacat atacaaaaaa
    16321 ttataattgt agctatgata gttgggctta caattataaa cttattaggt gtatctctat
    16381 caaaaattgt acaaaatgtt ataactgtag gtaaactaat tccattaata ctatttatag
    16441 gaataggtat attttttata aaaggagtta attttacttc ttcaacaatg gtacctccag
    16501 gagctggagc aacagagttt ggagcagcag cacttttaat gttttattca tttacaggat
    16561 ttgaatctat agcagttgca gcagaagata tggaaaatcc acaaaaaaat ataccaatag
    16621 caattataag tgtaatagta attgcatcaa taatatatat attaaatcaa gttgtatgtg
    16681 ttggtatact aggagattcg ttatctagta catctacacc agtggcagat gctgcaagaa
    16741 tatgctttgg aaatatggga gctggattgg ttacctttgg aacattggtt tctgttggtg
    16801 gaatttgtat gtgtggtgct tttgtaaatc caagaagttg tgtagcttta gctgatgata
    16861 aaatgttacc gagaatattt gcaagaaaag ataaaaaagg tacaccatat gtagcgataa
    16921 tagctactat gttaatcact ataccaatag ctctatctgg aagttttgct gaattagcag
    16981 caataagtgc agttgctaga ttcatacaat acataccaac atctttatcc gtattagtgt
    17041 ttagaaagaa aagaccagaa ttagtaggta cttttaaaac tccttttgga gcagtgattc
    17101 ctctaattgc tgtatgtgtt ggaacatggt tgttattcca agcatctatg catcaattaa
    17161 ttatgggatt aggagctttg gcaattggag taccattata ctttataatg aagtcttata
    17221 atagaaaaat ctatggtgaa aatttaaaga aaatagttta attatgttat aacaatttat
    17281 tgagttaaaa ttagacattg gcagaaaatt aaatacaaat aagtatctac aaggctatat
    17341 taaaatatta ttaatttttt attttaatat agccttatat tgttatcaat aaatgaaaaa
    17401 tattgtaaca acagaagaac aatagtatta tgtttataga caaaaaactt attaggaggt
    17461 aaatttatgc caacaataac attaaaaaat ggtgtagacg tacttattag agaaggtgta
    17521 agagaagatg ctcaaagcat tatagatttt tataatgaag tgggtggaga aactcatttt
    17581 ctgtcatttg gaaaggatga atataaaata tctttggagg aacaagaaaa tgctatagaa
    17641 tcagcaaaag catctgataa ttctgttaaa ctgattgcat ttatagatgg tgaaatagtt
    17701 gggattgcaa cgattgattc taatcaaaaa gcaaaaggaa agcatgttgg agtattagga
    17761 attgttgtaa aagaaaaata ttggggaatt ggattgggta aaaggcttat gttagacctt
    17821 atagaatggt gtaaatcaaa tggaataacc aagaagataa ctcttgtcac taatgaagaa
    17881 aattataatg ctataggatt atacaaaaaa gttggatttg aagtagaaag catactaaag
    17941 aaagaatgtt attataatgg tgtttatact gatttaatag gaatgtcact tttattggga
    18001 atataattag tgtacatttt atctcgaaaa aagtatatta agtatggtat aattaaaaaa
    18061 gaatataact taatataatt ttggaggacg aattatgaaa aatctgtgta aaatatcaga
    18121 tgattattta aacagcaagc ttaagtattt aaagttatta tcaaaacaat atccaagtat
    18181 atcaaaagca agtacagaaa taataaattt agaggctata ttaaatcttc caaagggaac
    18241 agagcatttt ataacagatg tacatggtga atatgaacca tttgtacatg tattaaaaaa
    18301 tggttctggt gtaataaaga gaaaaataga agaattgttt tcaaatacaa taagagacag
    18361 cgaaaaaaaa atgttagcaa ctcttgtata ttatcctgaa caaaagttag acctaataat
    18421 taagcaagag gaaaatatag atgattttta tagaataaat atttatagac ttatagaact
    18481 ttgtaaatat gcttctagca aatatactag gtctaaagtt agaaaactgt taccagaaaa
    18541 ttttaaatac ataatagaag aacttttgca tgagcatgtt aaaagtgaac ataaagaaga
    18601 gtattataaa agtatagttg aaacaatagt tgacatagga atagctaagg agtttataat
    18661 tgctatttct acagttatac aaaaattggt tgtagataga cttcatgtaa taggagatat
    18721 ttatgataga ggtccaagac cagatataat tgtcgataag cttattgaac atcattgtgt
    18781 agatattcaa tggggaaatc atgatatatt gtggatgggt gcagcctcag gtgaaaaaac
    18841 ttgtatagca aatgcactga ggatatcagc tagatatgcg aatttagaca ttgtagaaga
    18901 catatatgga ataaatctat tgcctttagc cacttttgct atagaaatgt ataaagatga
    18961 cccttgtaaa gaatttattc caaaggttaa tgaccaaagt gtaacaacaa cagaaaagtc
    19021 tttaatggca aaaatgcaca aagcaataag tataattcaa tttaagcttg aaggtgaggt
    19081 tatcagacga agacctgaat ttgaaatgga acatagactc ttgcttaata tgataaacta
    19141 tgatgaaggt acgattaatt taaaaggtaa aacatataaa ttaaaagata cttatttacc
    19201 tacaatagac aaaaaagacc catataaatt aactatggaa gaaagaaatg ttattgataa
    19261 attagtatct tcatttagag gtagtgaaaa actacaaaaa catgtttcat ttttgttttc
    19321 taaaggaagt atatatttaa aggctaactc aaatttactg attcatggat gtgtaccgtt
    19381 aaatgaagat ggaagcttta tgtctatgaa tataatggga aaagagtata aaggtaaggc
    19441 tcttatggat aagatggagt ctctagcaag agaaggattt ttctttaaag ataaggcaga
    19501 agaaaaactg tatggcatgg atattatgtg gtatttatgg actgggaaat gttcatcatt
    19561 atttggtaaa gatgatatga ctacatttga aagatacttt atagctgaaa aagagactca
    19621 taaagaaaat aagaacccat attttaaact tagagaaaat gagatggctt gtaaaagatt
    19681 atttgaagaa tttgatttag aacttgatga atctcatata ataaatggtc atgttcctgt
    19741 ggagagtaaa aatggagaga gcccaataaa agctaatggt aaaattctcg ttatagatgg
    19801 tggtttttct agggcttacc aaaaaactac tggtatagct ggatacactt tgatatacaa
    19861 ttctagaact ttgcaattag tatcgcatga accatttaat tctgctgaag aagccattgc
    19921 aaatgaaagt gatatcttat cgacaactgt tgtagtagag cataaggcaa agagaaaaat
    19981 ggtgagagat actgatgaag gaataaagat acaagaggaa atagaagatt taaaactact
    20041 tttaatggct tataagaaag ggctaataaa agaaatgtag atgttataga ggcacttcat
    20101 gattgaagtg tctttttgtt aatacttatt aaatatatta taaagattaa taatagtagt
    20161 ttatattata cttactaagt gaatccaaga taaatgggtt cacttttttt atttataaac
    20221 attattaaaa aaatatagat gttttggtta tattccttgt cctttataaa tagaattata
    20281 taaacaaagg aggaggacaa gagatgaata aactttctga tgaaataata aactctcttt
    20341 ctacaactat aagagaaaag gtagaaaaag tctcaaacaa taatctgaac atagaagaaa
    20401 ttaggttacg ctcacaaaaa ccattgatac taaatgcaaa ctctaaagat tatttttaca
    20461 atcaaaaaac tatgacactt gatttaaatc aacaaaactc atatgtagta acaagagaag
    20521 atgtagagca aacttttcaa ataatatgta aatattcaat acattcattt atggatgaca
    20581 taaaaaaagg attcataact ttgagaggag gtcatagagt agggttagtt ggaaaagcaa
    20641 tagtagaaga tggacaagtc aaaaatataa agcatatatc atctttaaat ataagggtat
    20701 caagggaaat tattggatgc tcggataaga ttttaagtca tatcataaag ggaaaaaatc
    20761 agattaataa tactttgata atatcaccac ctcaatgtgg aaaaacaact ttaataagag
    20821 atatagtaag aaatttaagt aatggtaatg aagattatgg atttaaaggt ttaaaagtgg
    20881 ctttagtaga tgagcgtaat gagatagcag gagcttatct aggtgttcca caaatggatg
    20941 tgggaataag gactgatata atagaaactt gcccaaaaga cttgggtatt acaatgcttt
    21001 taaggtctat gtcaccaaat gttattgtga cagatgaaat tggaagtgaa aaagaaataa
    21061 aggcattata cacagcatta aatggaggta taggattaat aactactgtg catggagatt
    21121 caatagagga tatacaaaat agaaaagaat taaatagact tctggataaa gagttattta
    21181 aaaaggttat aattctttct gcaaaaagag gagcaggaac aattgaaaaa atttatgatt
    21241 tagaagaaaa gagatggtat tttgcaaatt aaaataatta ttatagcttt cttgatagga
    21301 agtagttatt tgataggtga acaaatatac aaaacttata ctcgtagaca taaacaatta
    21361 aacgatttaa taagagtttt agaaattttg aggatggatt tatcttttgg actttataca
    21421 ttagaagaaa tctttaatag gataggtgga aataaagagt tttgtttttg gaaatttttc
    21481 tatcaaatat cagaaggact tcacaatgaa caaagtaaaa ctttggaaat aataatatca
    21541 gaaaatattg atgtcttaag taaagaaact tatctaggca ataaagaaat tgaggaactg
    21601 aaaaatctaa tacttacttt agggaaaagc gatatagagt cacaacaaag aatgatagat
    21661 ttatctatag aaaatttgaa aaaacaaacc tatgagacaa aggaagatat aaacaaaaaa
    21721 ggcgtattgt ataaaaaatt agtgacattc ataggtatag gtatatgtat tatattgatt
    21781 taatatgggg aggtttatta tggaaatatc attgatatta aaggttgcag gtgtcggtat
    21841 tctaatatca gtattgaata tgatattgga aaaaacagat agaaaagatt gggcaggtct
    21901 tactacatta gcaggtgtaa taatcgtatt gggtatggta ataacagaaa taagtgactt
    21961 gtttaatact gtaagaacaa tgtttcaact ttactaaaga ggagggagta atttggaaat
    22021 aatgcaatta ataggaattg caatcatttc aactacactc tgtttagtaa ttaaaaaaga
    22081 tagaccagag atagcaaatt tcatagctat aataactgga gtaataatac tattgtcagt
    22141 tatgtttaag ctaaatttta tagtagatag tatacaagat ttggctaaca aggctaacat
    22201 cccaactatg tatatatcat taataataaa attaattggg atagcatact taatggaatt
    22261 tgcgatacaa ctttgtaaag attgtgggga aggaaacatt gcttcaaaac tagaatttgg
    22321 aggtaaaata atagttatgt caatgtcttt ccctatactt ttgtccattg tagaaatggt
    22381 ggtgaatata attccataga tgtgtatctt ggaatgaata taagtatatt agagagcttt
    22441 cgttgtattg aggtttatag agtgaagaga ggcatggttg cttatgaaaa aaagattttt
    22501 accaatgatt ataggttttt tatttacttt cttttttgta aatatctttg ctattatgat
    22561 atttgctaat gaagtaccaa gtagtgaaga taaagaatat ggtgaaacaa aaaatagcat
    22621 agataagtat atagatggtc aattagacaa gctagatata aatgagattc aagactatat
    22681 aaataaagaa atagttatta atgatgttaa tttaaaatca tttgtaaaag atttaattag
    22741 tggagaaaaa aatatcttag atttattcaa taaagatgga ttgaagatat tgatgtttga
    22801 tgaatttaaa gcaagtttaa aggtagtagc agtaattttg gtactggctc tgttatcatc
    22861 tattttaaag agtctggaaa actctttttc atcaggtgca gtaagtcaaa tagcaacata
    22921 tataatattt atcacaatgg tatcacttac acttattggt tttaaagatg tattacagat
    22981 ttgctatgat gcaatagacc atactgtagg tcttatgcaa gttataatgc caatattaat
    23041 aacattttta cttttaattg gattcccaat aacgtcgact actttgaatc ctatcttcat
    23101 aggaggagta acatttataa atgtattttt taaaaatttt ctatttgtat ctattactgt
    23161 tgcatttgga attttaataa taaacaattt atcaaaaaat ataaggctaa agagattttt
    23221 ctcctttgta aaacaaataa attatgtttc tattggagct atgtttactg tttacttagg
    23281 tcttgtatcc atacaaggat tgtatgtaac cagttttgat aaattcagtg taaagactgc
    23341 taaatttgct ataggtaatt ttattcctgt tgtaggtgga tttgtatctg actctgtgga
    23401 tattttatta tcttcatctc agcttataaa aaatatattt ggaggaatag gacttatctt
    23461 acttgttgga atttgcttac ttccagtaat aaaaatttta tcagtaatag tagtgtacaa
    23521 attagcagca ataattgtag agcctgttgg agaggatgga atatctaatt ttttaaatga
    23581 agtggcaaat ttaatgataa taatgctagc atcagtaatt gctataacag taatgttctt
    23641 tgtaactgta gctatcttga cctcgataag tgtcgtaagt caagggtaat ttatatatca
    23701 gtgaaaaata attacattta atctaatgtt tttagagagg aggatgctat gttagagggc
    23761 ataaaggcat ggatagttag tgttttgata ggagcattta tagtaaatat agtagatatg
    23821 attttaccaa gttctaagat aaaaccttat gtcaacctag ttttaaactt tatgtttgta
    23881 tttattgtaa taactccagt agtaggattt ttttcaaaag atatgagttt agaagataga
    23941 atattaaaat ctatgggaaa ctacaacaag cagtatgtag atagtacaaa tgctttggca
    24001 aaagagactg gaaataatag tctatccaag ggatatgagg atgggttaaa agaagtactt
    24061 aaattaaaac ttgatgaata tggttatgat ttagaagata tagagcttaa tggggcaaat
    24121 ataaataata taaaaataaa agaaaagaat aatagtgcaa aaagtaataa cagttccaat
    24181 gaagaaaata aaaataatag tactaaaaga gaggaagaaa acataaactc taatgacaaa
    24241 gaaaattcta aacaagtctt caaaaaaggt acagagtatg ggttgaacct aaatgaagaa
    24301 aaattaaaaa acgacttaat aaaggtgctg gatgtttcta tagaagatat acaaattgat
    24361 aaataggagt tgggagaatg ttgaaaaact taaatgacaa agataagaga aaggtatatt
    24421 cactaataac tatagcagga atatgtgtgg tttcattagt attattatct tgttttccta
    24481 gtagcaaaag cgataaacaa gttggaaaaa cagagactaa taaaaatgct gaaaagcaag
    24541 tcactaaaga acaagaaaaa gatgatttag agtcaaaatt aacagcaata ctatctaaaa
    24601 ttgatggtgc aggagacgtt gatgtaatgg ttacatttga atcaagtgaa gaaatacaac
    24661 ctgcttttaa ttcaaataat acaacagaaa caacagagga aaaagatgca caaggtggag
    24721 aaagaacagt tacgacttct agtgaaaata aaacaatgat aacttcaaat tcaagtgacc
    24781 ctgtagttat aaaaactaca gagccaaaga taaagggagt gatagtggta gcaagtggag
    24841 caagtgaccc aagtgttaaa gagacacttt atagtgcagt acaaacatca ttacaagtag
    24901 ctggtcatca agtagaaata tattctaaat aaatacatat aaccgggggg ataaatgaat
    24961 gaagtttaat tataagggaa gaggatttgt aataataact ttaactgcaa tgttagtggt
    25021 agtaggaaca gtgaattatc aactaagtaa gaaatcttta ttagaaacat caaaagagtt
    25081 taaggcatat gaagaagcac agttgcaaaa aaatactgat gagagtgata gtagtaagac
    25141 agaggattct agtaatgtag ataaacaaga tggtaaggaa agtgctgata ttgatatagt
    25201 tgatagcaaa gctagtaaag taaaagaaaa ggcaactgag acaagtaagg aaataaaagc
    25261 tcaattatca tcagagaaaa atatgaaaaa ggcatcttat attttagata tgaaaatgaa
    25321 tagagaaaaa caaagaaatg agttagttca agatttaaat gaaatgataa ataatccatc
    25381 tacaacagaa gaatctagaa aagaagcttc taatatgaaa ttaaatatag ttaaaattca
    25441 agaaaaagaa cttcaaatag aaaacctttt gagtacaaaa gggtatgaag aagcgttagt
    25501 ttatataagc gataacaaag ttaatgtagt tgtaaatgaa gctaaattag aaaagaaaga
    25561 tgcagcaaaa atatttgatt tagttgcaga acaagccaat gtgaagtatg aaaatattaa
    25621 acttacaaat aataatagta ataagtaaag tataaacctc agaattaact gaggttattt
    25681 tcttgcaaag tagacttgta tttggtatta ttatacttat aaaaatagtt ccttaaaatc
    25741 aggaggtaag ttttaatatg gaagataata aatttggaca agtaaaaata tctaatgatg
    25801 taatagctac aatagctggt ttagcagcat tagaagttga aggcatagaa actacagcaa
    25861 cattaacaga taagttgtta aaaaataatg gagtaaaaat acaaatcgaa gaagaggatg
    25921 ttaatttaga tgtaatggtt acgataaagt atggaatgtc aataccagat acagctttta
    25981 aagttcaaga aaatgtaaaa aatactgttg agacaatgac aggactgaag gtttctcaag
    26041 taaatataca cattcaagga atcagcttta agaaggataa agttgataaa gaagaagcaa
    26101 aagcaactaa gaaaaattaa tattagccct ctgaaaagtc agagggtttt ttgctggtaa
    26161 ttgattttat atggataatt aaacttaaag gagaattttg attgatgaaa aaagataggg
    26221 cacaaaagag tacaacaaga gaatatataa tgaaacttat ataccaaata aatattaata
    26281 aagaagattt tgaaacttta gaagataaag tagataattt tttaaaagat aattcagaac
    26341 atattataaa tagatataaa gaacttgcac ttcaatactc aaaaaataca aatttaaagt
    26401 tagaagatac tgaaattgaa gatgttatag acaaaaaata tataaataca gtatgcaaag
    26461 cattgaaaga aaatcatgac aaaatagatg agttaataaa taagcatgct aaaaactgga
    26521 cagtagatag aatgccaaag gtagatgttt ctatacttag attgtctgtg tgtgaaatac
    26581 tttatttaga cactcctaat aaagtatcaa taaatgaagc agtagaactt gcaaaaatat
    26641 attgtgatga taagtctcct aaatttataa atggtatatt gggaagtgtt gttgatgaaa
    26701 ttgggaaata atataataat tggaattgat actagctgct atactacttc tatagcagct
    26761 atctctttag ataagaaagt tatattcaat gaaaaaataa tgttagaagt aagggataat
    26821 tcaaaaggtt taaggcaaag tgaagctgta tttcaacata tcaataattt agggattctt
    26881 agtgatagga taaaatcttt taaagataag tttaatgtag aaggagtttg ctcatcaaaa
    26941 aaaccgagac ctgttgaaaa ctcatatatg cctgtattca atgtaggtca taattttgga
    27001 aaattattgt ctagtatata tggttgtagg ttttatgaga ctactcatca agagaatcat
    27061 attgaagcaa gtttattaaa tagtaaatta aaaaataata ataaatttat ttctgttcat
    27121 atgtctggtg gtactacaga gatactttta acaagcaaac aagatagtca tcacaatgta
    27181 tgtgatacta atttaggtaa aattgccaaa ataagtatta aaaaagatga taaaagtaag
    27241 ttatacaata attttggata taatatagat ataattggag gaagtaaaga tattagcttt
    27301 ggtcaactta tagatagagt aggaattaag cttggatata aatttccttc aggaaagtat
    27361 ttggatgaaa atgctttaaa ttgtaatctt aaaatagaaa gtggtctgaa aacttctgta
    27421 agagatgggt atatgaattt atcaggatta gaaaatcagg taaataagat tataaatgat
    27481 aatggagata atactaatca aaaagaatat atatctaaat tggtattaga ttcagtagtt
    27541 agaaatatgt ttaaatcttt agtgtattta tgtgaaactt ataatgtaaa tgaagtagta
    27601 tttgctggtg gagtttctgc aagtaagtat attcttagag aattaagcat gaagttaaga
    27661 aaaaagcata tagaagctta ttttacagaa ccacaatatt caactgataa tgctgtcggg
    27721 tgtgctataa taggattgaa taacttttta ggagaaagag tatgaaacta agagctttag
    27781 atataagtga agcaaactca tatattaaaa gaattttaat aaacgaccca atactttcta
    27841 atcttaaagt taaaggagaa atttctaatt ttaaggttca tagtagtggg aatgtatatt
    27901 tatcacttaa agatgaaaca tcaaagttaa actgtgtaat ttttaaaagt aattttaatc
    27961 gcaatttaaa attagataat ggtgtaaaaa ttatagctaa tggatatata tcagtctatg
    28021 aaagagatgg tgcgtatcaa ttatacatta atgaaattga aatagaggga ataggaaatt
    28081 tacacataga gtttaataga ttaaaggaaa aacttaataa agaaggatta tttgacccta
    28141 aatacaagat acctatacct aaaatgccaa actcaattgg cgttataaca tctccaacag
    28201 gtgctgttat aagagatata ataaatgtaa taaaaagaag atatccgaaa gttaatataa
    28261 agctttatcc agtaacggtt caaggagata aatcagctga agaaatctgt gaagcaatta
    28321 gatttttcaa tcatatgaag aatgttgata cacttatagt tggtagaggt gggggttcga
    28381 tagaagaact ttggtctttt aatgatgaaa tggtagcaag agaggttttt aattctcaaa
    28441 ttccgataat atcagcagtt gggcatgaaa cggattttac tatttgtgac tttgtgtctg
    28501 atatgagagc cccaacgcca tcagcagcag ctgagattgc tactccatca ttggatgata
    28561 taaactataa acttggaaac ataaaaagta ggatgagtaa gtctcttaca aatcaaatag
    28621 agctagacca atataggtta gaaacagtat ttaataagat aaataactat ttagattcgt
    28681 acacaataaa agataaagtt atacaattag acaaaatata tgataaaata atttttggaa
    28741 tagaaaataa tttaaaatta gaagatgaaa aattagtaaa aattggagca ttactccata
    28801 atttaagtcc attagctact atggatagag gttatagtat aactcaaaaa aatggaaaag
    28861 taataaatag tataaaagga ttaaaaataa aggatagtat agatatagta ttaaaagatg
    28921 gaaatttaga atgtatgata gataaaattg aaaataaaga gggatagtat gaatttaacc
    28981 tacgaagaag cttataaaag attagaaagt attttaagtg aattagaatc taaaaatgct
    29041 agcttggatg aatctttaag cctatatgaa gaaggtataa gcctttataa acattgtaat
    29101 aagcttttag atgatgctaa attgaaaata agtaaattta atcaattggg aatagaagaa
    29161 gactttaaaa tagaggagga ataaaattgg aatttaaaca atgtctaaaa gaaaaagcaa
    29221 gttttgtaga aaaagtactt aaagaatata tgcctaaaga agaagggtat caaaaaactg
    29281 ttattgaagc tatgaactat agcttaagtg ctggaggtaa aagattaaga ccaatactta
    29341 cattggaggc ttgtaaaata gttggaggta atgaagatga agctattcct tttgctattg
    29401 caattgaaat gatacataca tattcactaa tacatgatga tttgccagct cttgacaatg
    29461 atgatttaag aaggggaaga ccaacaaatc ataaagtata tggagaagct atggggattt
    29521 tagcaggaga tgctttgtta aactatgctt ttgaagtaat gcttgcaggc tcaatcaata
    29581 aagaaaatcc agaaaagtat ctaaaagcta taaatgaaat tgccaaaggt gctggtatat
    29641 atggaatgat aggtggtcaa gtagtagatg tagaaagcga aaataaacaa attggaaaag
    29701 aaaaattaga ttatatacat atgaataaaa ctgctgcaat gatggtaggc tgtatgagag
    29761 caggagctac tataggtgga gcaaattctg aacaaatgga agaaataact aaatatgcaa
    29821 aaaatatcgg attatcattc cagatagtag atgatatact agacatagta ggagatgaag
    29881 ctaagttagg taaaaaggtt ggaagtgata tagaaaatca taagtcaaca tacccatctc
    29941 ttttaggttt agataaatct aaagaaattg cacataattt gattgatgaa gcaaagaaaa
    30001 gtatagaaaa attatctgat gatgtagatt ttttaaaggg attggctgaa tatataatag
    30061 atagagaata ctaaaatata cagctaaaat cgaggaggga aaaatggatt ttttttcgga
    30121 gatttttaac aatggggctt tgggaataag tctgattgct tgtttcttag cacaatttat
    30181 aaaaatattt actggtaagg aaaaaagaat tgagttatca agaatactta tttcaggagg
    30241 tatgcctagt tcccatagtt cttttgttac aagtttagct acagtggtag gtattgaaaa
    30301 agggtttaat tctactgatt ttgctataat taccgtgctt gccttaatta taatgtatga
    30361 tgcagcaggt gttagacggg ctgttggtaa acaagctact atattaaatc aaatggtggc
    30421 agatatacag catggaaagc atatagagca gaagaaatta aaagaattaa taggtcatac
    30481 accactagaa gtttggtttg gagctttact tggaatagta acagctttaa tattgatgta
    30541 aattgaaaat ttacagaata aaggtgagga taatatgtat aaatatttag ataaagtaaa
    30601 ttctccaaag gatataaaaa atatgagtat agaagaaatg gatttacttg caaaggatat
    30661 aagaaagttc ttagtaaaat ctgtttcaaa gacaggagga cacttagctt ctaatctagg
    30721 agtggttgag ttaacattag cacttcataa agtatttgat agccctaaag ataagattgt
    30781 atgggatgta gggcatcaat cttatgttca taaaatagtt actggaagaa aagattgttt
    30841 tgtatctttg agacagttta atggactaag tggttttcca aaggagaatg aaagtcctca
    30901 tgatattttt gatacaggac atagcagtac atctatttct atagctacag gtattgcctg
    30961 tgctagggat ataaaaaaag aaaactatag tgttatatct gtgataggag atggttctat
    31021 aacaggaggt atggcacttg aagctttaaa tcaattagga tatatagata ccaatatgat
    31081 agtaatcctt aatgataatg agatgtccat agataaaaat gttggaggaa tgtctaaata
    31141 tctgtcaagt attataagaa actcaacagt tgagaaaatg acagatgaag ttgataaaat
    31201 tttaaatgtt acacaaacag gtgaaatttt atctaagact gcacatagat ttaaagataa
    31261 actaatgtat agcttttctc ctcaggactg ttcatttttt gattcattag gtataagata
    31321 ttatggacct atagatggcc ataatactaa agaactaata gatatattga gaaaagctaa
    31381 acacaaaaaa gggcctgttc ttttacatgt aattacgaaa aaaggaaaag ggtacagatt
    31441 tgcagaagaa caacctgata aatatcatgg agtatcaaag tttgatataa agacaggtgt
    31501 tacatctgca aaagttaagt ctatgtcaat tagtgttgga gaaaaacttg ttgatatggc
    31561 taatagtaat gaaaatatag tagcaataac agctgcaatg ccatctggta cagggttaaa
    31621 cctgtttgaa agtgcatatc caaaaagata ttatgatgtt ggtatagctg aacaacatgc
    31681 aacaggattt gcagcaggtc ttgcaaaaaa tggtatgaaa ccttactttg ctgtgtactc
    31741 atcatttttg caaagggcat atgaccaagt tattcatgat gtatgcataa caaaaaagcc
    31801 agttactttt cttatagata gagctggatt agttggaaat gatggagaaa ctcatcatgg
    31861 tatgtttgat ttaagttact taaattctat tccaaatatt gtagtaatgg ctccaaaaga
    31921 cacaagagag atggagctta tgatggattt gtcattaaaa ttagattgtc cactagcaat
    31981 tagataccca agaggaagta gttattacct agataaggga gagtatggag agattgtact
    32041 gggtaaatat gaggtattag atgacggtca agatacagtc atactttgta ttggaagtat
    32101 ggtaaaacat gctttagagg ctaaagaaat attatcaaga gaaggaataa atccgacaat
    32161 agttaatgct agattcttaa agccaattga tgaaggtatg ttaaaagcat tattaaaaaa
    32221 tcataaaaat gtagttacta tagaggataa tattgtaact ggaggatttg gaagtagaat
    32281 aaataagttt attatagata atgaatataa cgtaaatata cttaatatag caataccaga
    32341 agaatttgta aaacatggca atatagatga attatatgat tttgtaggat tatcgccaaa
    32401 aagtattgct gataaaataa ggaaattagt tattgagtaa aaatctaatg tagatattgt
    32461 aaattaaaat ttatataaaa tatagaattt atcaattaga ataaatatca agtgtgttac
    32521 ttcaagctac tttagttatc attaaagtag cttttaacag aaaggtaatt gtatgaaaaa
    32581 aagaatagat ttattattag tagaacaagg gtactttgaa agtagagaaa gagccaaaaa
    32641 agcaataatg gctggattgg tttttgttga taatcaaagg tgtgataagg ctggtacaga
    32701 agtaaaagaa gattgtagta tagaagtaaa aggaaatcca ataccatatg tcagtagagg
    32761 tggtttaaag cttgaaaaag caatgaaaaa ctttgatttg actattgatg gaaaggtatg
    32821 tatggatata ggggcatcca caggtggttt tacagattgt atgctcaaaa atggagcaat
    32881 aaaagtattt tctatagatg ttggttatgg tcaacttgca tggaaattga gacaagatga
    32941 tagggttgtg tgtatggaaa gaaccaatat aagaaatgtg acaatagaag atacaaagca
    33001 gtttgctgat tttgcgtcaa ttgatgtgtc ttttatatcg ttaaagttag tacttccaaa
    33061 ggcaaaggag ttagtacgtc atgatgggga agttgtcgct ttaattaaac ctcaatttga
    33121 agcaggaaga gaaaaagtag gtaaaaaagg agtagttaga gaaaaatcta cacatataga
    33181 agttataaaa atgatttctg atttctcagt cgaaaatgga tttgagattt tagggttaga
    33241 tttttctcct attaaaggtc ctgaaggtaa tatagaatat ctaattcatt tgagaaatgg
    33301 aaatgaagga tatgaatttg atggtgagac atacaataac aaaatagtag aagttgttga
    33361 agcttctcat aatttagaca aataggttaa aagggggctt agatttgatc ctcgaattat
    33421 atatgaaaaa ttgtgcctta gttgaagaac taaggttgaa tattgataaa aacttaaata
    33481 tacttacagg tgaaactggt tcaggaaaat ctataataat agatgcactt ggactttgtt
    33541 taggtgataa atatgatagg tcttttttaa gaaaaggaac agataaggga cttgttgagg
    33601 ctgtattttt ttcagacaat agatatctta aaaaaatatt agaagaaaat gatataagta
    33661 tggaagatga taatttattg gtaataacta ggttgatata ctcagatggt aagagtactg
    33721 ctagagtaaa tggaaggact gttaaagtgt ctcttcttaa agaaattgca tctacactta
    33781 tagatataca tggtcaacat caaaatcaag ctttgtttaa taaagatact catttaaaat
    33841 ttctagacct ttttggagaa aatgaattgg aagagtttaa aattgcttat aaaaatgtgt
    33901 accataagta tagtgaagta aaaaaagctt taaattgctt gacagaaaat aaagatgaga
    33961 tgcaaattca acgagaaata gatttattaa gatttcaaat aaatgaaatt gaagctgcaa
    34021 atctaaataa gaatgaatat gaagatttat taaaacaaag agaagtttat agaaatagtg
    34081 aaaagatata taataatcta aattcgagtt atagcaagtt acataatggt gagtataatg
    34141 ttattgacct aataggtctt gcatctaagg aacttaatga catatctaaa tatgacagtg
    34201 ttttaagtga atatagtgat actgtagaaa gaatcatgta tgagttacaa gacatatctg
    34261 gtgagataag aaattacaaa gataatatag actttgaacc ttatgagcta gagcagattg
    34321 agcttagaat cgacgagatt aataacttaa gaagaaaata tggagacagt atagatgata
    34381 tatttgagta ttatggaaaa attaaagata gattagatga aatattaaac agagatgaaa
    34441 gagttgaaca gttaagaagc aaactgatga atatagaaga agatttaaaa ataaaagcca
    34501 gtaaattgac taaagcaaga atagaagtag caactacttt agaaaaaata cttcttgatg
    34561 aattaaaaag cttaaatatg aaaaatgtaa tgttcaaggt taattttgaa aaaggtccat
    34621 ttactttaga tggagtagat gatattgaat ttatgatatc ttttaactta ggagaagata
    34681 ttaagcctat atataaagtt gcatcaggtg gtgagatgtc cagatttatg ttagcgttta
    34741 aaaccatatt ggctgatata gatgatattg atacattagt gtttgacgag atagatactg
    34801 gaataagtgg gatagcagca cagatagttg gtgagaaatt gagtgatata gcaaagaaaa
    34861 aacagataat atgtataact catttacctc aaattgctgc aaatgcagac actcattatt
    34921 gtatagagaa agatacttca aataatagga cgtttaccaa tgttagtaag ttaaatgaat
    34981 ctcaacgaaa aaatgaaatt gccagactta tagcaggtaa caatataaca gaaaaaacta
    35041 ttgaacatgc aagtgaaatt attgagttgg ctaaaaaatg ttagtgaaaa acaagcattt
    35101 taataggtgt aaagaatttt tatagaaatg ctttacatct attttttatg atgaaattat
    35161 tttaaaatat ctagtgccta tattgaagaa aagtagattt aaaaatagaa gaaaatttgt
    35221 ataaatttgt aactttgaaa tttctaaatg cttaagtata atattattta ttaaggtagt
    35281 agtgaggggg acataaaatg aataaaaaag atactatgca cttaattttt acaattgtaa
    35341 tttttatttt attatggtat attgtggtag cagtaagtga gattaataaa aatattgaaa
    35401 gttgtagtaa taaattagat tatttgtcac aagagattat gagtactaaa tcggatatta
    35461 gtaatactat aaatgaagaa atgtcaaaaa gttatataac aaagagtatt gacttaaaag
    35521 taaaaaaaat cgagaaaaaa gagtgtgtta ttgatgtaga tgttcaatta agtaaacttg
    35581 gaaaaaatgg aaaagtattt tttatgtata aagaagatag tgataaatgg aatgaaactg
    35641 agatgacaaa acatggagaa ttatcatatg catgtgaaat taagataaat acaggaagtg
    35701 aatatgacta taaagtagtt acaagtggag ctatatctga aagttctgat gtgcaaaaat
    35761 tgactaaatc tgattatatg cctgaacaac ccatatttaa ttctgggata agagataata
    35821 gagaatactt tattgaaata gtagaaaata gtaatctttt caatcatgag agtgaaaagt
    35881 caaaaaataa agtttatgat aatattagat tagaaaaagt agatattatt gtaaatgaag
    35941 gaaaaaaaga cactatatac aaagccaaat tagctgaagg cttagatgat ggcaaaacta
    36001 ataattcaaa tagaaatcaa gtgaattatg aagtaagttt tcctaaaagg gatattgatg
    36061 acataattta tattaaagct aaacttacat acaataatgg agtagaatat acaaaggata
    36121 taactgaaga tataactatg ggattacaag atttagaaaa ataaatacat atttaaataa
    36181 atattcaaat agatttgttc aataaaatag agtataaata aaactgtagt agttttactt
    36241 atactctatt aaaaaattat aattttaatt ccatatatct agccccttca attggattat
    36301 aagtataagg ttctatatca taaaaaccaa acttttcata taatccttgt gcacttttca
    36361 tgcttggaag agtatctaat ctcatgtgtg tatatccaaa ttttttagct tcttcaatga
    36421 tttcttccaa tagtattttt cctattttta atcctctaaa ttgatttctt acatataatc
    36481 tttttaattc acatatttta ccttcaagtt tttttaaagc aacacaacct gccaaatttt
    36541 catcaacaaa tgccaatatc aatgagcctg aaggtttttt atattttcct ggaagtgttt
    36601 ttaattcatt attaaagtct tgaaaacata agtctatatt tagagaattt gaatattcag
    36661 taaatagaag ttttacgttt tcaaggtttt ctaaatcttt ttcttcaaca aattgtaaag
    36721 ttatattttg agccatttta tccgccatta tataaacatg tttatataat gacttcacct
    36781 ccttgatata tgtatatttt tcttaatatc aattagattt taataccatt actttttcac
    36841 caccatagtc aagctctcca ttaaactcaa atccaaaatc tttataaagc ttaatagcca
    36901 ttttattgtc ttcgaatata ctaagatata gttcatcaca gttatattca tttttaagat
    36961 gatttacaag aaaatctaat gatgcttttc catatccttt accttgatat tttgaatcaa
    37021 tcataaatct atcaagccaa actctatcat cttctaaacg accatacata gcaaatccta
    37081 ttaaagaatt ttcatcatat attccaacag gtacccaagc tgttataaat ttagattcag
    37141 caatagaaag tgcattgctt tctatataat ctaactgact tttatctaca gacaaactaa
    37201 ctaaatcatg ccaattattt atgtctgcaa ttcttacatt taatttcaag atattaccgc
    37261 ccttcaaact ttttgttaca atctaatact atcatattat gtattaaaaa tcacttatta
    37321 agattttaaa taaacttttt aaatgaataa attctcagga aaaaggaatt ataaatacat
    37381 aataatacag cataggagta gatggatata tgaacaaaat aaataaaaat ttcaaaaata
    37441 tttggataat attaggagct gtatcgattt ttaccatggt tataattttt aataaaaatt
    37501 gtaatgttaa taaagagact caaatagaag cttttacaat taagaatcat gataaaaaat
    37561 ttgtatatcc tatgggaaat ataattggag taaaagcaaa tacagatggt gtattggtct
    37621 taggatatga ggaagaggat gtagactata ttggaggcat acaaatcggt gataatatag
    37681 taaaaataga taataaaagg ataaaaaata gtcaagatgt atcagagatt ttgaataaga
    37741 ttaaaaaaag taaagttgaa gtgacttttg agagaaaaaa taagtataaa acagaagtaa
    37801 tagagactaa aaaagaaaat ggcaagtata aacttggatt atgggtaaga gataaaatat
    37861 ctggaattgg aactatgact ttttacgacc ctagtatgga aaagttcaaa gcaatcggtc
    37921 atgccataaa agattcggat actaatgaac tattaaagat aaagcaagga tatatatata
    37981 agccagaaca attaaaaata gtaaaagcta gtaatgaaaa agtaggtaaa ataaaaggag
    38041 attttaatga cagcaattta atgggtaatt tctcaaataa ttcagagtta ggtataagtg
    38101 gtaatattac agaaaaccat aataaagagt ttaatgtagc aaataaagaa aagcaattaa
    38161 tagaagttgg aagaccgcaa gatgtaaaaa taggagatgc agtaattctt tttgaggata
    38221 aaaacaaaaa tataacaagc tatgatataa aaatagaaag tatagtatat gataaaggaa
    38281 attatagaga tatggtaata aaagtagtag atgacaagtt attggaatac acaggaggta
    38341 tcgtacaggg gatgagtgga gctccaataa tacaaaataa taaaattatt ggtgcaataa
    38401 ctcatgtttt tagagataat ccgaaaaaag gatatggtat ttttatagat gaaatgataa
    38461 aattgtaggt gaggcattaa aaaattttat tattttatca attatctagg aggaatataa
    38521 ttttggagtg tcgaatatgc tttagagtag ataattagga agcaattgtg taaaaagttt
    38581 agttttctgt aataagaaga tgttttttaa tggggggatt tttagtggaa aaaatcaaaa
    38641 tagttttagc agatgacaat aaggattttt gtcaggtatt aaaagagtat ttgtctaatg
    38701 aagatgatat cgatatatta ggcatagcta aggatggaat tgaagcatta gacttagtaa
    38761 aaaagacaca accggactta ttaatactag atgtaataat gccacatcta gatggattag
    38821 gtgtaattga aaaattgaat actatggata tacctaaaat gccaaaaata atagtactat
    38881 cagcagtagg tcaagataag ataactcaaa gcgcaataaa tctaggagca gattactaca
    38941 tagtaaagcc atttgatttt gttgtgttta taaacagaat tagagagcta gtttctaata
    39001 gagttacaca agttgagcca aaacctagac cagttcaaga gactcaaatg actagaagtg
    39061 attttgttaa gaatgtagga aatatagaga cagaaatcac aaatataata catgaaatag
    39121 gagtaccagc tcatataaaa ggatatttat atttaagaga agcaataaaa atggttattg
    39181 ataatgttga gcttttaggt gcagtaacaa aagagttata tccaagtata gctaaaaaat
    39241 tcaatacaac accaagtaga gttgaaagag caataagaca tgcaatagag gttgcatgga
    39301 gtagaggaaa agttgacaca ataaatcaat tatttggata tacggtacac aatactaaag
    39361 gaaaaccaac taattcagaa tttatagcaa tgattgctga taaattaaga ctagaacata
    39421 gtatggttaa ataaacaaga cataaaaagt aaggcttttt aattaaggca ttggctataa
    39481 atgcgtatta caagcagcga aacggtataa ccactagggt tataccgttt cgctatttta
    39541 ataaatataa aaattttctt tattatttgc ttactatatc aatataataa ttttattata
    39601 ctatggatat agtatgtgtc tttacaagtt gtaaactgac agtggtttat tttttaatat
    39661 aaatattgac tttgatgcag gtaaactttg tatttttaag cgtattatgg aatatgttaa
    39721 ataaaaaaat gatgaaatat agtattgtaa atgccaaaga tgcaaaacaa acttaaaaca
    39781 tttattttat tgttaagtaa tgctataata taatgtgatt ttaataatga tagtggaggt
    39841 ttaaatatga gagtcgaggc ccctataaaa gtagatcgaa aaaccaaaaa acttgctaaa
    39901 agagttgaaa gtggggaaat agcagttata aatcatatag acatagatga agttgctgca
    39961 aactctttag tagaagctaa aataaaactt gtcataaatg cggctccttc tataagtggt
    40021 aggtatccca ataaaggtcc agggatatta actgaaaata atatactaat aattgataat
    40081 gttggagaag agttatttga agaattaaaa gaaggagaaa ctatagaagt tgtagatgga
    40141 aagatatata gaaaaggaaa atttttaggt gcaggagaag ttttagacaa gtatgaggtt
    40201 tcacaccaaa taaaggctgc ctatgagaat ttagctgtag aattggatag atttatagat
    40261 aatactatag attatgctaa aaaagaaaaa ggatttatac ttggagaagt agaaattcca
    40321 aaagtgaaaa ctaattatgc taataaacat gtattgatag ttgtaagagg tcaggactac
    40381 aaagaagatt taagtactat gctttcttat atagaagagg taaagccagt attggttggt
    40441 gttgatggag gagcagacgc tttaattgag tttggatata ctccagatgt gattgtaggt
    40501 gatatggata gtgtaagtga tgaagcttta aaaaaggcta gtgaaatagt agtacatgct
    40561 tatacagatg gaagagcacc aggtcttaag agggttgagg aattaggctt agatgcagtt
    40621 gtatttcctg cgccaggaac tagtgaagat atagctatgc ttatcgccta tgagtataag
    40681 gctgagttaa tagtagcagt tggtacacat tcaaatatga ttgattttct tgaaaaagga
    40741 agaaaaggta tggcaagtac ttttttagtt agactaaaaa taggttcaaa gcttatagat
    40801 gctaaaggcg ttaatttgtt atacagaagt aaattaaaaa taaagtacat atgggcatta
    40861 atagcaacag cattatttcc tgtgttggta gttgcatcat tgtcaccagg agttcaacag
    40921 ttcatacaac taatgcaatt aaagtttaga gtattattac agatgtaaat tttaggagga
    40981 taaatatgca tattaatatg aaatattata tagtcactat aggggctata tttatagctt
    41041 tgggtatagg catgttagta ggatttaatt taaataataa tcaacaatta agtgaacaac
    41101 aagcaaatat aataaatgat ttggatgata agtttgatat cttaaaagaa aaaaatgata
    41161 aacttgacag tgacttagca agtgtaaata gagattatga agaggcagtt aattttataa
    41221 ataaaaatgt agataagatt ttagctggtt cgctgaatgg taaaagtata ggaataatat
    41281 ctgctaatga aaatgacgat tatacaaaaa atatagaaga cataataaat aaatctaatg
    41341 gaagcattgc atttaatata atattaaaag aaaacataac aaatccagaa aagttaaagg
    41401 aaatgtcaac taaactaggt acagatgtaa aaaatgcaaa tgatgcagtt aattatataa
    41461 ttgacactct taaaaaggaa gatgcaagtg acattttaac atatcttcaa gaactagatg
    41521 taataaagtt taattttatt ggagatacat atctaaaata tgattccgtt gtaatagctg
    41581 gtggaaatga tgcaaaagac agtgcaaaac agtttgaaaa aatagaaaaa tttgtagttt
    41641 caaaacttaa atcagagaat aaatatttag tagaagttca aaatacagga gttaaaactt
    41701 cttatgtaga actatattct aaaaacaaag ttgcaactat agacaatatt gatgaaggta
    41761 taggaagtat atctctagcg attcttctac aacaaggtaa tatagttggt aattttggta
    41821 gattggatac tgcaacttca ttgttgccat ctattaaata gaaataattt taaggagaga
    41881 aaaaatggat tcttatataa gtataataat accagcttat aatgaagaaa gtagaataaa
    41941 atatacatta gaaagcatat tggacattaa gcaaataaat gaaataatag ttgtggatga
    42001 tggttcaatt gacaatactg ctaaggttgt atctgagcta gaaaatgaaa agattaaatt
    42061 ttttaagtta gataaaaata gaggtaaagg atatgcttta aattatggta tcaaattagc
    42121 aatgaaaaat gcagatatta taggttttct ggatggagat ttaggaagtt ctgctaaaga
    42181 agttgaaaaa ttaatagtac ctatattaaa tggtgaggcg gatgttacaa tagccaagtt
    42241 tcctcctgca aagagaaaag gaggtttggg atttgtaaaa ggtttagcta aaaattctgt
    42301 gtttgagatg actggtgttg agttagatgc aactttgtca ggtcaaagaa tatttaaaag
    42361 agaagtttta gaaaagtttg atgaaatacc ttttggatat ggagtagaag ttggaatgac
    42421 cattgatata ttaaagtatg gatttaaaat taaagaagta ttagtaaata tgactcataa
    42481 tgaaacagga agaaatttaa aaggttttat tcatagaggg aaacagtatt atcacataaa
    42541 aaaggtttta ggacaaaaaa agaaagaatg gaggtaatat cttagtgaaa gattatatac
    42601 tttataccat tttattatta actggattat ttggaaccta tgctgtaatt ccactattta
    42661 aaaatttatt gataaatgga aatgtactta gaccaaacta taaaaaagat atgataccag
    42721 taagtatggg aattgtattt ttgcctatga ttgtaatcaa tggaataata cttgctttct
    42781 ttactacaga atttaaggat ttagcgtata tatttatgtt tatgtttggt atgatatcca
    42841 tgttttttgc agggatacta gatgatgtca ttggaaatag agatgtaagt ggtttaaagg
    42901 ggcattttaa aagtttattt aaaggaagtc ttacaacagg aggttttaaa gctttatttg
    42961 gtggatttgt agggcttata gtttctgttg ctatatcaaa agacataatt gatataatag
    43021 taaatacact aataatagct ctatcaacta atttaatgaa tcttttggat ttaagaccag
    43081 gaagggcaat aaaagcatat ttattcataa tggtaattat atttttaaca ttgacaggat
    43141 ttgttcaagt tcttccatta cttatagtac caaatgtact tgcatacttt aattatgatt
    43201 taaaagcaag ggctatgatg ggagatactg gttcaaacgt tcttggaata tctattggaa
    43261 tgctcatttc ttttgggtat ccatttaaca ttagattagg atggttaata tttgtagtta
    43321 ttatgcatat attcactgag aaatactcat taactaagat aatagaaaaa aataaactat
    43381 taaattttat agataaattg ggaaggtagt attatgataa gcaaaagagt ggggattgtt
    43441 gaatcaatag taagtcaaac tgaaactcta gatgatataa gagtaaatat aaatggagaa
    43501 atacaaaagg catataatta tccaaaaata tctggaacta taaatatagg tgacgaagtg
    43561 gttttaaata caacagcagt agaactgagc ttaggtacag gtggatacca ttttgtaata
    43621 actaatttaa ataatataga atctacttta actgaaggtg gacatataat gaaacttaga
    43681 tatacgcctc ttcaaataaa agtggattct gttgaggaac aagaaagtat ttatcatgat
    43741 aaatttgcga attttaaagg attagaaggt ctttctgttg tagtagggac tcttcatagt
    43801 atgttaactc catttgcagc ttcatttaaa agaaataatc caaataaaaa attagtgtat
    43861 ataatgactg acggagcatc tcttccaatt tatttaagta aaaatgtgga tactttaaaa
    43921 gaaaaaaaat taatagatag tacaataact ataggaaatg cgtttggtgg agattatgaa
    43981 tgtataaata tatatactgc attaattaca gcaaaagaaa tactaaaagc ggatgcagta
    44041 tttgtcagta tgggacctgg aattgctggg acaggaacaa aatatggatt tacagggatt
    44101 gaacaaggct ctattcttga tgccgtaaaa aaacttgaag gaagagcaat tgcaattcca
    44161 agaattagtt ttgctgacaa acgagaaaga catcaaggta tttcacatca tagtatgact
    44221 gtatttaaag aaattgtaaa tgtaaatgta gacataccaa taactatata tgatgatgaa
    44281 aaattgaact ttataaaaga gcaaatcaat ttaaatggtt tagacaaaaa gcataatata
    44341 atttatatag ataataataa aacgaaagag gatttagact attttaatct aaaagtaaag
    44401 agtatgggaa gaaattatga acaagatgag gcatttttca aagctgcaag cacagcagca
    44461 tattacttaa tggaggttta agatggtatt agaagaaaaa actattagta gtgatagggt
    44521 ttatacaggt aaagtaatta ctttaaaggt tgatactgtt gaaattccag ggcaaggata
    44581 tcaaaaaagg gagttagtag aagttggtgg agcagtagga atagttgcaa tcacagatga
    44641 taataaagtt gtgttagtta agcaatttag aaaacctata gaaaagccaa tctttgaaat
    44701 tccagctggt aaactagaaa aaaatgaaag tccaaaagaa tgtgctgaaa gagaattaaa
    44761 agaagaaact gggtatagtg caaaaaatat taagctaata cataaatttt tcacttcagc
    44821 aggtttttct aatgagataa tgtttgtata tttagcaaca ggtcttactc caggagagaa
    44881 taatttagat gcagatgagt ttttagatgt ctatgaaatt gagcttgaag aagcatataa
    44941 catggtatta aaaaatgacg ttgaagatgc aaaaacatct attggattat tattagtaaa
    45001 agatatgttt aaaaattaat atttataaaa tgaataaccc tttgtcctaa agcatattat
    45061 tttataaagg acaaggggga attaaattga gaagaacata tagaaaaaat gattttagag
    45121 aattaaatag acaaataatt attataggat tgttgtttat gatgtctatt gttgttgggt
    45181 cttacataaa taaaatattg ccaggttcta gtaacaatat attaaacaat ataaatcctg
    45241 ctgtagagta ttataatcta aatataagca taaaagatac agtgatacaa aatttaaaat
    45301 cagatgcaat ctttatgggc tcaatagctc tattaagtct atttgtagta acaattccag
    45361 cagtgttggt agcttttgta ttaaaaggga tgtcaattgg ttatacaata aatagttgta
    45421 tattggcatt aaaatttaaa agtataaaaa tgattttaat aatattattt aaaaatttga
    45481 ttattattcc atgtgcaatc atattagcat taattagttt aagttatttt aaagaaatgg
    45541 tttatgaatt taaaaagaaa aatagaaaaa atatgcaatt tttaattaag aggtatatac
    45601 taaatattat aattataata gcactatcat tggggttgca attaatatta aatactgcaa
    45661 gtattggtat tattaagttc ctagctaaat aaaggagttt ataatggata ttatagaggg
    45721 atatatagat tacataaaag ataaaaaaaa attatcagag aatacagtgg catcatactt
    45781 tatggatata aaaaaatata tggaatatat aaatcaaaaa agcattaaac tagtagatat
    45841 agtagaaaat gatattataa gttacttaat aaagttggaa aaagataatg tatcaatagc
    45901 tacaatagcg aggatgatat cctctataaa atcttttcat gattatttat ttttaaatca
    45961 tatatgtaca aataatcctg caaaggatat aaaaaaacct aaaataaaaa aagaaaatat
    46021 aaatatactt acagaagaag aaattgaaag actattaaat tttccaaagt taacaacgcc
    46081 taaattgatt agggacaagg ctatttttga agtattatat ggtactggta taaaagtatc
    46141 tgaattagta gagatgaata tagaggatat tgacctagat attgattata tatattgtaa
    46201 ttcatgttgt aaaaatcaaa gagtgatacc actttgtgat attacaaaac tttacttaga
    46261 aaagtatcta aaagaagcaa gacctaaaat ggctgtagaa ggtgaaaaat cattattcgt
    46321 aagttcatta ggtcaaaggt ttacacgaca aggtctttgg aaagtcataa aaaaatattc
    46381 taatctagcc aatatagata aaaatataaa tcctacaatg cttagacatt catttgctat
    46441 tcatttgtta aatgaaggtg ctaacattgc tgttgtaagt aaaattttag gaaatgttaa
    46501 tttatctagt ttgcaagtat atttaaatca tatagataaa aacgtgagaa gagaaataaa
    46561 ggagaaacat cctagaaatg atgttgatgt agaattacaa aaagcagaag ctaaatagac
    46621 tatatagcaa attttataat ttagaaggag catatttata tgagcagagt aatttggatt
    46681 gtaattgata gtgttgggat aggagcatta cctgatgctg aaaaatttgg tgatagtaag
    46741 gatgtaagta cccttggaaa tatatttaag gaatatccag atattcagat tcctaatatg
    46801 agaaatttag gtataggaaa catagatggt atagattttt ttgaaagtat aaaagaacca
    46861 ataggttgtt ttggaaagtg taaggaaatg tctcagggaa aagatactac tacaggtcat
    46921 tgggaaatga ctggtataat agttgacaaa ccatttaaga catttgaaca tggattctct
    46981 aaggagataa tagaagagtt tgagaaaaaa actggaagaa aagtagtagg aaataaacca
    47041 gcttcaggaa ctgtaataat agatgagtat ggagagcatc aaataaaaac tggagatgta
    47101 atagtatata catcagctga tagtgtattt cagattgctg ctaatgaaga agtaattccc
    47161 ctagaagaac tctataatat gtgcaaaatc gctagggaaa taatgatggg ggataatgca
    47221 gtagctagag taattgctag accattcata ggtaagaaaa aaggtgagtt tgtgaggact
    47281 tcaaatagaa gagattattc actagaccca tttgaaccaa cagttcttga taatataaaa
    47341 gaatcaggac ttgatgtttt agcagttgga aaaatagaag atatttttaa tggaaaagga
    47401 attacagatg ctatccacac taaaagtaat atggatggag tggatgaaac tttaaattat
    47461 atgaaacaag ataataaagg tctaatatat tcaaatctcg ttgattttga ttctaaatat
    47521 ggtcatagaa gagacccaga aggatataag aaagctcttg aagagtttga tagtagactt
    47581 cctgaaataa tggctaatat gagagaagat gatattttaa taatcaatgc agaccatgga
    47641 aatgacccaa catataaagg tacagaccat acaagagaat atatacctgt tatgatttat
    47701 ggcaataaga ttaaaaaagg ttttaattta ggagtaaaag atacttttgc tgatattgga
    47761 gcaactgttg cagatatact aaatgtaaag ttgcctaaac atgggtcaag ctttaaagaa
    47821 gatttatttt aaattataaa atgataaaaa cttagcttaa taggatggag agattggaat
    47881 gtttgaaaag atagcacaaa gtagtaaatt tataaattct aaaagtaata taaaacctaa
    47941 gattggtttg atattaggtt ctggactagg tgatttagca aatgatatag aagaccctgt
    48001 cacaattaaa tatagtgaaa ttccaaactt ccctgtatca acagtagcag gacatgctgg
    48061 acaacttgta attggaaaac ttgaaggaaa agaagttatt gctatgcaag gtagatttca
    48121 ttactatgaa ggatattctc agaaagaagc tacttttcca gtaagggtta tgaaagaatt
    48181 aggagtcgag attcttatag taactaacgc tgctggaggc gtaaataaag aatttaaagc
    48241 aggagatttg atgcttataa gagaccatat aaactttagt ggaagtaatc cattagtagg
    48301 aaaaaatgat gacagatttg gtgctagatt tccagatatg tcggatgcat attcttctaa
    48361 atatgtagat gtagttaaaa actgcgctaa agaatgtaat atagatgtca aagaaggcgt
    48421 ttatatgttt tttagtggtc caaactatga aacaccagca gaagtaagaa tggcacaaat
    48481 tttgggagct gatgctgtag gtatgtctac tgttccagaa gtcatagtag cctctcactc
    48541 taatatagga gttattggta tatcttgtat aacaaatatg gctgctggca tcttaaatca
    48601 accactaagt catgaggaag taatagaaac tactcaaaaa gttaaatcag aatttatgaa
    48661 tttagttaaa acaacggtta aaaatttata agttaaagct tattatagat tttttgagga
    48721 agtttataat aaaataggta atgtttaatt tgaaaagaat taataaaaag ctgtcttgat
    48781 atagaaagtt atttctaata aaggacagtt tttttataat actacaacat ttgaaagtgt
    48841 tttaaagttt aatatttatt taaattatta taggttagaa tggagatttc aattatgaga
    48901 atgtatgata ttataaaaaa aaagagagat aacctagagc ttagtaaaaa agagataaac
    48961 tactttatag aaaactattc aaagggagat atacctgact atcaagcttc tgcactatta
    49021 atgtcgattt atttaaataa gatgaataaa caggaaactg tacatcttac agaagcaatg
    49081 atgaactctg gagacatgat aaacctatct gagatagatg gaataaaggt agataagcat
    49141 agtactggtg gtgttggaga taaaactacc atagctctta tacctttggt tgcttcatgt
    49201 ggagcaccag ttgcaaagat gtcaggtaga ggtcttggtc atactggtgg tactattgat
    49261 aaactggagt ctataccagg tttttcgaca gaaatggaga taagtaaatt tattgattct
    49321 gtaaataagt ctaaaatagc agtttgtggt caaagtgcaa aggtagcaat tgcagataag
    49381 aaaatatatg ctcttagaga tgttacagca actgttgata atatatcatt aatatcatca
    49441 agtgtaatgt ctaaaaagtt agcttcagga gctgatgcaa ttgtgctaga tgtaaaaact
    49501 ggtgatggag catttatgaa gacattagaa gagtcttttg agttagctaa atctatggtt
    49561 gatataggta ctggtatgaa tagagataca atagggattg taacagatat ggatgaacct
    49621 ttaggttttg cagtgggaaa ctctttagaa gttatagagg caattgagac attaaaagga
    49681 aatggaccta aagaccttgt tgagctatgc gaagtattag gagcatatat gttggtttta
    49741 tcaaaggtag cacatgattt tgaagatggt gttgaaaaaa taagagaatc aataaaatct
    49801 ggtactgcta tacaaaaact caaagagttt atagagaatc aaggtggtaa taaagatgta
    49861 gtagatgatt attctttatt caaaaaatca aagtataaat atccagttaa atcaactaaa
    49921 tcaggttata taagtaaaat taaagctgaa gatgttggtg tatcggctat gattcttgga
    49981 gcaggtagag aaactaaaga tgatatttta gatttatcag cagggataat tttagaaaag
    50041 aaagtaggag attatgtaaa tgaaggtgaa gttttagctt acatgtatta taatgatgaa
    50101 caaaagcttt cattggcaaa gggaagattt attgattcct actctattgt agattcaaaa
    50161 atagaaaaaa ataaattaat atatggtatt gtaactaaag aagagattaa aaagttttag
    50221 aaaattttag aaaagtgatt ataaaaggcc taacatgtca aaaataatgt taggtttttt
    50281 attttttgat aaactataaa ttataccaaa aatatatatt ttaaaacatt ttttacaata
    50341 taaataagtt tattctacaa gatgattgaa aattaaagta tctagtttta taatagaaaa
    50401 tggataaata tacataagga gtagattata ttatgaac
//
LOCUS       DAGSHT010000008        50007 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGSHT010000008 DAGSHT010000000
VERSION     DAGSHT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.009"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            158..1027
                     /locus_tag="MJB72_000420"
     CDS             158..1027
                     /locus_tag="MJB72_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY8261598.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1253..2119
                     /locus_tag="MJB72_000421"
     CDS             1253..2119
                     /locus_tag="MJB72_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8261599.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            2311..2508
                     /locus_tag="MJB72_000422"
     CDS             2311..2508
                     /locus_tag="MJB72_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261600.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            2511..2954
                     /locus_tag="MJB72_000423"
     CDS             2511..2954
                     /locus_tag="MJB72_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261601.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(3141..3228)
                     /locus_tag="MJB72_000424"
     tRNA            complement(3141..3228)
                     /locus_tag="MJB72_000424"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Leu,seq:caa)
     gene            3380..6028
                     /gene="polA"
                     /locus_tag="MJB72_000425"
     CDS             3380..6028
                     /gene="polA"
                     /locus_tag="MJB72_000425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBY8261602.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            6037..6639
                     /locus_tag="MJB72_000426"
     CDS             6037..6639
                     /locus_tag="MJB72_000426"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBY8261603.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            6632..7186
                     /locus_tag="MJB72_000427"
     CDS             6632..7186
                     /locus_tag="MJB72_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBY8261604.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            7186..8901
                     /locus_tag="MJB72_000428"
     CDS             7186..8901
                     /locus_tag="MJB72_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8261605.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            8974..9186
                     /locus_tag="MJB72_000429"
     CDS             8974..9186
                     /locus_tag="MJB72_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBY8261606.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            9387..9986
                     /locus_tag="MJB72_000430"
     CDS             9387..9986
                     /locus_tag="MJB72_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261607.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            10214..10585
                     /locus_tag="MJB72_000431"
     CDS             10214..10585
                     /locus_tag="MJB72_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY8261608.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            10717..11991
                     /locus_tag="MJB72_000432"
     CDS             10717..11991
                     /locus_tag="MJB72_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY8261609.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDIVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQPNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            12102..13046
                     /locus_tag="MJB72_000433"
     CDS             12102..13046
                     /locus_tag="MJB72_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261610.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            13262..14599
                     /gene="rsxC"
                     /locus_tag="MJB72_000434"
     CDS             13262..14599
                     /gene="rsxC"
                     /locus_tag="MJB72_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBY8261611.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            14622..15599
                     /locus_tag="MJB72_000435"
     CDS             14622..15599
                     /locus_tag="MJB72_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBY8261612.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            15599..16168
                     /locus_tag="MJB72_000436"
     CDS             15599..16168
                     /locus_tag="MJB72_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBY8261613.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            16181..16774
                     /locus_tag="MJB72_000437"
     CDS             16181..16774
                     /locus_tag="MJB72_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBY8261614.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            16789..17364
                     /gene="rsxA"
                     /locus_tag="MJB72_000438"
     CDS             16789..17364
                     /gene="rsxA"
                     /locus_tag="MJB72_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBY8261615.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            17380..18351
                     /locus_tag="MJB72_000439"
     CDS             17380..18351
                     /locus_tag="MJB72_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBY8261616.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            18571..19155
                     /gene="maf"
                     /locus_tag="MJB72_000440"
     CDS             18571..19155
                     /gene="maf"
                     /locus_tag="MJB72_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBY8261617.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            19200..19859
                     /gene="radC"
                     /locus_tag="MJB72_000441"
     CDS             19200..19859
                     /gene="radC"
                     /locus_tag="MJB72_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBY8261618.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGAGLYFSFKENMI
                     I"
     gene            19980..20990
                     /locus_tag="MJB72_000442"
     CDS             19980..20990
                     /locus_tag="MJB72_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBY8261619.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            21000..21893
                     /gene="mreC"
                     /locus_tag="MJB72_000443"
     CDS             21000..21893
                     /gene="mreC"
                     /locus_tag="MJB72_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBY8261620.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            21908..22396
                     /gene="mreD"
                     /locus_tag="MJB72_000444"
     CDS             21908..22396
                     /gene="mreD"
                     /locus_tag="MJB72_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBY8261621.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            22408..25386
                     /locus_tag="MJB72_000445"
     CDS             22408..25386
                     /locus_tag="MJB72_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBY8261622.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            25484..26167
                     /locus_tag="MJB72_000446"
     CDS             25484..26167
                     /locus_tag="MJB72_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBY8261623.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            26191..26988
                     /gene="minD"
                     /locus_tag="MJB72_000447"
     CDS             26191..26988
                     /gene="minD"
                     /locus_tag="MJB72_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBY8261624.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            27006..27290
                     /gene="minE"
                     /locus_tag="MJB72_000448"
     CDS             27006..27290
                     /gene="minE"
                     /locus_tag="MJB72_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBY8261625.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            27375..28505
                     /gene="rodA"
                     /locus_tag="MJB72_000449"
     CDS             27375..28505
                     /gene="rodA"
                     /locus_tag="MJB72_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBY8261626.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            28546..28959
                     /locus_tag="MJB72_000450"
     CDS             28546..28959
                     /locus_tag="MJB72_000450"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBY8261627.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            29444..29773
                     /locus_tag="MJB72_000451"
     CDS             29444..29773
                     /locus_tag="MJB72_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261628.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            29777..30568
                     /locus_tag="MJB72_000452"
     CDS             29777..30568
                     /locus_tag="MJB72_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261629.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            30698..31518
                     /locus_tag="MJB72_000453"
                     /pseudo
     CDS             30698..31518
                     /locus_tag="MJB72_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(31730..32923)
                     /locus_tag="MJB72_000454"
     CDS             complement(31730..32923)
                     /locus_tag="MJB72_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBY8261630.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            33242..35086
                     /locus_tag="MJB72_000455"
     CDS             33242..35086
                     /locus_tag="MJB72_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBY8261631.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            35088..35789
                     /locus_tag="MJB72_000456"
     CDS             35088..35789
                     /locus_tag="MJB72_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBY8261632.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            35807..37228
                     /locus_tag="MJB72_000457"
     CDS             35807..37228
                     /locus_tag="MJB72_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBY8261633.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            37329..37640
                     /gene="rplU"
                     /locus_tag="MJB72_000458"
     CDS             37329..37640
                     /gene="rplU"
                     /locus_tag="MJB72_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBY8261634.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            37655..37972
                     /locus_tag="MJB72_000459"
     CDS             37655..37972
                     /locus_tag="MJB72_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBY8261635.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            37986..38276
                     /gene="rpmA"
                     /locus_tag="MJB72_000460"
     CDS             37986..38276
                     /gene="rpmA"
                     /locus_tag="MJB72_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBY8261636.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            38387..39664
                     /gene="obgE"
                     /locus_tag="MJB72_000461"
     CDS             38387..39664
                     /gene="obgE"
                     /locus_tag="MJB72_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBY8261637.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            39716..40084
                     /locus_tag="MJB72_000462"
     CDS             39716..40084
                     /locus_tag="MJB72_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBY8261638.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      40176..40282
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            40385..41590
                     /locus_tag="MJB72_000463"
     CDS             40385..41590
                     /locus_tag="MJB72_000463"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBY8261639.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     gene            complement(41791..42357)
                     /locus_tag="MJB72_000464"
     CDS             complement(41791..42357)
                     /locus_tag="MJB72_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY8261640.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(42637..43896)
                     /locus_tag="MJB72_000465"
     CDS             complement(42637..43896)
                     /locus_tag="MJB72_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBY8261641.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            44021..44734
                     /locus_tag="MJB72_000466"
     CDS             44021..44734
                     /locus_tag="MJB72_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBY8261642.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            44852..46126
                     /gene="larA"
                     /locus_tag="MJB72_000467"
     CDS             44852..46126
                     /gene="larA"
                     /locus_tag="MJB72_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBY8261643.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            46143..46931
                     /locus_tag="MJB72_000468"
     CDS             46143..46931
                     /locus_tag="MJB72_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY8261644.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            46945..48165
                     /locus_tag="MJB72_000469"
     CDS             46945..48165
                     /locus_tag="MJB72_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBY8261645.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            48176..49567
                     /locus_tag="MJB72_000470"
     CDS             48176..49567
                     /locus_tag="MJB72_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBY8261646.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
BASE COUNT        19732 a         5435 c         9385 g        15455 t
ORIGIN      
        1 tggattttgc acaactggtc attattaatg taaaagtgga atattattaa taaataataa
       61 ttacaaaatt gtaatcttta tttattaaat aaatgagtaa aataatatat taatgggtat
      121 ataattaaga caatactagt ttggggagga taaaattatg gattataaaa caatcatatt
      181 aaatagaaag tctgttagag aatataagaa cacagatatt aaagaagaat atcttagtga
      241 aattaagaaa tactcagaat cttgtaagaa actagttcca gaaatagatg ttgatgtaag
      301 aattatgaat agaagtgaag tttttaataa tttagataaa gtagcagggt acaaaggaaa
      361 tatgatagat tctccaagtt atattataat attatctgac ataaaagata attacattga
      421 aaattcaggg tacataggag aaagtataat ctttaaagta acagaacttg gaattggttc
      481 ttgttgggtt acttttgaag atggaaaaaa ggtaaaagaa aaacttggaa ttaattcaga
      541 taaagaggta acaggaatta tagctattgg atatggagaa aatgtaagta atgctaaagt
      601 attaaatgct acaaaaactg ggcaaaatta ttcaaaatct gatatgcaag ttgtaacaga
      661 taaggggtct actagactag gtgtagaaga tgttgtttat atgaacgaat ggggaaatgt
      721 tgcaagtata gaaatcttag aagaaagagc acttcttgat gcatttcact atgcaagatt
      781 agctccatct acattaaata gacaaccatg gagatttata gttgatggag gcactgtagt
      841 tttagctgtt agaaaagatg gtcatactaa tttatatgaa gaaaaaatag acattggtat
      901 agttatgctt tattttgcaa caataataag tgcaactatg tttgatttaa aatggaattt
      961 aggtactcca gacaaagatt ataaagtgcc agaagactat aagatagtag gatattgtaa
     1021 tatataagat agtgtaatac ttaatttaac tacacttaaa ttaattttta taaaaagttc
     1081 aataacattt tataatataa tgttattgaa cttttttatt gtataataca atttttatat
     1141 ttattgtatt gattgaaaaa tacttatgaa tttaaatgct tacaattttg tgcatgaata
     1201 gagaggtata ttattttatt aaactgtata atttgcttgg aggtaatatc aaatggaatg
     1261 gataaataaa ttaaatcaag ctattactta tattgaagaa aatctcgaaa gtggagtgga
     1321 ttatgcagaa gcagcaaaaa ttgcatgttg ttcaaccttt cattttcaaa gaatgttttc
     1381 atatattgca gaagtaccat tatctgaata tattagacga agacgtatga ctaaggctgc
     1441 atttgaactt cagaacagta acatcaaaat tttagaacta tctgagaagt atggttacga
     1501 ttcaccaaca tcttttaata gagcttttca aaatatacat aacatttctc catctgcagc
     1561 tagagcaaag ggtgttgttt taaaagcata tcctaagatg acattaagca tttatgtaaa
     1621 aggaaatata gaaatgcaat atcgtattgt agaaaagaaa gggtttcgta ttgttggtat
     1681 aaaagaaagt atgaacatga ttgtagaaga gtgctttgag aaagttccta agttgtgggc
     1741 taaatgcata aaaaatggaa caattgataa actttcagag ttaataaata atgaaccttg
     1801 tggattgctt ggagttagtg tgtgtacaaa tagtaagggt ttagattatt atattgctgc
     1861 tccaacagat aagcctgtat tagaagatac tcatgaatac ttaataccat caggcacatg
     1921 ggcagtattt gaatgtgttt ctccaatgcc aaatgctctg gcaatacagg aattgcaaaa
     1981 aagaattatt actgagtggt taccaagttc tgggtatgtg tatgctaatt taccagatat
     2041 tgaactttat cccaatggcg atatcaatgc tcctgattat actacagaag tttggattcc
     2101 aattcagaaa ccaagttaaa attaataaat aatatggaat taataaataa cattaaaatt
     2161 aataaacaac atgaaattct aaaattacag gctgaaaata gtctgttatt tttttgtaaa
     2221 tatggaaagt aagctttaca taaatggaat atagttgtat aattatggaa agtagacttt
     2281 ccgtagttga gatattaaag ggggatagat gtgaaaaata gattagaaga aataagaaaa
     2341 gaaagaggca taaaacaaga agaacttgct tctattttgc aagtatcaag acaaactata
     2401 ggttcattag aaaatggtag gtataatcct tctatcatct tagcctttaa gatagctaga
     2461 tactttgata tgagtattga agaaatattt atttatgagg aggagtaaaa atgaaaaaga
     2521 atatgttata tgttggaatt atatatttta tttgtggaat tttacttgtt ttattggcta
     2581 catttactga gttttcattt gaggctttta tttggggatt atctggagct gctttaggtc
     2641 caggtgtatg tatgattctt caatatatgt attggagtaa acctgaaaga gcagtagatt
     2701 atgaagaaaa gataaaaaat caaaggatag agatgaatga tgaaagaaga attatgttac
     2761 gtgataaagc tggacgtatt actaatcaaa tcatgtccta tgttcttgta attcttatat
     2821 ttattgtttc tatattgagt gtattttctg taatggtaat aagcaagtgg gttttagttg
     2881 ttcttggatt gcttatttta tttcaattta tttgtagtgt tgttgtatac aataaattag
     2941 ataaaaagtt gtaataagtt atgatataaa atagaaaatt atataaaaat aaattagttt
     3001 ttaaattaat gctagaagat aaatttatat aattatataa attttaaaaa gacacagtaa
     3061 tttcaaaaaa taacttaaaa tttaaatcaa aaataaaaaa actcctaatc tattgaataa
     3121 atagctttag gagtttttaa tggtgcccga ggccggactc gaaccgacac ggagtattag
     3181 tccggtggat tttgagtcca ctgcgtctac caattccacc actcgggcat gtcatgtata
     3241 ttattatagc aaaaaaatat attgtttgca acatatttaa ttttaatata ctattattat
     3301 tatgggaata ttaaataaca gtatattggt atataatata tatgttacaa aaatagataa
     3361 tttttaggag tgatataatt tggaaaaaaa attaattata atagatggaa attcaataat
     3421 aaatagagct ttttatgctc ttccagaaat gaataataaa gaagggctaa agaccaatgc
     3481 aatatatgga tttacaacaa tgctatttaa aatgatagat atatataaac caacacatat
     3541 aagtgtagca tttgacagaa aagcacctac atttagacat ttagaatata aagagtataa
     3601 ggctggaaga aagggaatgc cagatgaact ggcagaacaa ttacagccat taaaagacct
     3661 tttagataaa tttagaataa atagattgga aatagatggc tatgaggcag atgatattat
     3721 aggtactgtt tctaaaaaag cagaagaaaa tggatataag gtatacatag taactggaga
     3781 caaagatgct attcaacttg cttcagacaa tacaactact cttataacaa aaaaaggagt
     3841 tggagaagtt gaagaataca actttaattc tgtaattgaa aaatacgaga tgactccaac
     3901 acaatttatc gatttaaagg gtcttatggg agacaagtct gataatatac ctggagtacc
     3961 tggaataggt gaaaaaacag gtataaagct tataaaagag ttttctagta tagagaatct
     4021 aatagagaat acagataagc ttaaaggtag tgtaaaaaag aaaatagagg aaaacaaaga
     4081 aatagctatt caaagtaaaa gacttgcaac aattataaga gatgtgccaa tagaagtgga
     4141 tttagatagt atgatttttg gcgattatga taaagatgaa cttttagata aatttagata
     4201 ctttggattt acaagtctct tatcaagatt agttgatttg gtcgattcag atgacacaga
     4261 acacgtagaa gaaaaaatag aaatattaaa attaaaagat atagaaaaat ttatagatga
     4321 agtaaacaaa aaaggacaag ttattttaaa aactgttaga ggacaaggta atatacttga
     4381 aaaaaatata atatatattt ttataagtgt agatggagaa aaaatatatt atgtagattc
     4441 aaatgaagtg tcaaagttag atagtatatt ttcaaataat gaaattaaaa agtatggata
     4501 taatttaaaa gatgattata tatctttaaa accatataat ataaacttag aaaatatgta
     4561 ttttgatata acaatagctg agtacttaat agattcaact tcatctacct catatgattg
     4621 tagtgcaata gctatgaagt atttatcaaa aaaggttaaa tcaaaagaag aattattagg
     4681 aaagggtgtt aaatctaaaa actatgagga tttagaattt gaggatttag cagagtatat
     4741 gggtcaaata atatgcacag ttaaagaaac agttcctatg atggaaagaa gtctaatgga
     4801 tatgagtatg gatggtcttt tttatcatgt agagatgcct ttagtagaag tcttaggtca
     4861 tatggagtat gaaggtataa aagtagacaa aagtatattg gatgaattga gtgttgaatt
     4921 taaagaaata atagctacat tagaaaagga aatttatgaa ttatcaggag agccattcaa
     4981 tataaattca ccaaaacaat tgggtgttat attatttgaa aaattagagc ttccaataat
     5041 taaaaaaact aaaactggat attcaactaa tgcagatgtt ttagaaaaat taagagataa
     5101 gcatccaata atagataaga taactgagta tagacaaata gtaaagttaa attctactta
     5161 cgttgaagga cttttaaata taataaatcc agaaagtaat agaatacatt catcatttaa
     5221 tcaaactata acgactacag gaagaatttc atctacagaa ccaaatcttc aaaatatacc
     5281 tataaaaatg gaaatgggta gaaaaataag aaaagtgttt attgcagatg ataactgtaa
     5341 gttggttgat gccgattact cacaagtaga gctaagagtt cttgcacata tgagtcagga
     5401 tgaaaatatg atagaagcat ttaaacatca tgaagatatt cataccaaaa cagcttcaca
     5461 agtttttaat gttcctatgg aagatgttac tagtgaattg agaggtgcag ctaaagctgt
     5521 aaactttggt attatatatg gtaaaagtga ctttggtcta gctgatgatt taaatatacc
     5581 tgttccaaag gcgaaagaat atatcgaaag ttattttgct aaataccata aaataaaaga
     5641 gtttatggac acaacagtag aaaaggctag tgaggatgga tatgctgtaa caattttaaa
     5701 cagaagaaga tatataccag aaataaagtc aagcaatttt atggaaagaa atagaggaaa
     5761 gagatttgca atgaatgcac caatacaagg aagtgcagca gatataataa aaattgctat
     5821 gataaatgtt cataaaaaat tggaagagaa caatttgaaa tcaaaactta tactacaagt
     5881 tcacgacgag ttgatagttg aagcaattga tgatgaaata gatatagtta agaaaatagt
     5941 aaaagaagaa atggaaaatg ctgtaaatat ggatgttcac ttagatgtag atttaaatgt
     6001 aggtagttct tggtatgaga caaagtaggt gattttatgt tgatattagg acttacagga
     6061 ggaataggct gtggaaaaag cagcctatca aatatattta gaaatcttaa cattcctata
     6121 gtggatgctg atattatatc caggaagata tttgaggata agttgttatt ggaaaaagta
     6181 tttgtccatt ttgggcaaag tataaaaaat gatgatggaa ctctaaatag aaaagctctt
     6241 gggaaaatag tttttagtga tgaagaaaaa cttaaggaat taaataactt gactcatcca
     6301 cgaataagag aaaaaataat aagtgaaata gaaaaattga gaaaaaaagg cgaaaatata
     6361 gtagtgttag atgcggcaat attggtagaa agtggttttt tagatatggt tgacaagcta
     6421 ttagtagtta cttgtaaaca agaagtacaa attagtagaa tacaaaagag agataattgc
     6481 agtgagcagg aagcacttag taggataaat tcacagatga gtcaggaaga aaaatccaaa
     6541 tatggtgatt atataataga taattcggga acaataactg aattagaaag taaagcacat
     6601 aaatttattg agtacatgaa ggagaattgg cgtgaatagt aagaaagtat tgattctatc
     6661 tatttttata atcttatttg gggcactatt aatggaaagc aaagtaatac ataaattttt
     6721 atatcctaaa aaatattcag agtatgtaga aaagtattcg aaagaattta atttagatga
     6781 aaatatagtt tacagtgtta ttaaagccga aagtaagttt aatagttctg ctgtttcaaa
     6841 aaaggaagca aaaggattaa tgcaaatatt agacataact agagattggg gagcagagga
     6901 actaaattta aaaaatgtgg atattttcga cccagagact aatataagac ttggctgttg
     6961 gtatttaagt aagttataca aagaatttgg taaattagat ttagtgatag ctgcatataa
     7021 tggtggttca ggtaatgtga aaaaatggtt agaaaataat gaatatagta aagatggcga
     7081 aaatctacat gatatacctt ttaagcaaac ttcaaaatat gtagaaaaag taaaaaataa
     7141 ttacgaacat tataataaga tatatggcaa gaaaggaaaa aactaatgaa gagaataaaa
     7201 gtcttaacag tcgttttagc tataactctt atggtagctg gttgtagtaa tactaaaaca
     7261 aaacaaagta gtagtgattc aagtttacaa agtcaaagtt cgatagatgc taagtatata
     7321 aacttaacaa tggtaactcc aaaaactata aatcctataa ctaatactga taagtctgtt
     7381 ggttatataa tgaatttagt ttatgatagt ttatttacta tagatgagaa ctacaatgta
     7441 attcctcaat tagttaagga gtataatata gctcaggatg gaatgagtat agatataaag
     7501 ctaaaagatg caaagtggca tgatggaaaa aatgttactt caaatgatgt aaactatact
     7561 attggtttaa ttcaaaaaag tgcagatagt ccatataatg aatttactaa aaatatagct
     7621 tctgtgaata ttaaatcaga taaagatttt acaataaaat ttaaagctag atatgctttt
     7681 tcaattgata gtttaatttt tccaattgta tctcaaaatc atttagattc aaaagatgta
     7741 aatgacaaga ataataatat gataggaaat gggaaatata aaatagaatc atatacagaa
     7801 agagaaggaa tggttttatc tgtaaacaaa gattactatg aagaagttcc aaaaaccatg
     7861 aagaacataa aagtaggtat ggtgcctaat gaagatgcaa ggacatctat ggttatggct
     7921 cttgatagtg atataaccaa tgttacatta aatgatttaa gtaagtttca agaaaaagag
     7981 tttaatataa caaaatatca aggaagagat tatgagtgtg tattatttaa ttataataat
     8041 ccttttttca aagatgttaa ttttagaaaa gcaattgcac actctataga taaagataga
     8101 attattagtg aaggttatat ggatgatgct actccagtta attttccact taattcaaaa
     8161 tcaaaatact ataatagtga aatgaaggat ttagagttta ataaagataa ggcgatagag
     8221 tgtcttgcta aggtagaata tgcaaatgta aactcagtaa atcaaaatga caataaagta
     8281 aaaaatcaga aaaaatcagc taaaaagaac tcaaagaagc ttacgccaga agaagaagca
     8341 aaagctaaaa aagcagaaga agatagaatc aaaaaagaag ctgaagagaa aaaaaataga
     8401 gaaaaagagg aagtaaagaa aagtctttct gaaatgaact taaagataat agtaaacaga
     8461 gataatagtg agagaataaa aactgcaaac ataataaatg aaaacttaaa agctatagga
     8521 ataaatagta cagtaaatca gctgtcagat aaggatatgg aaaatgctct taattctaaa
     8581 aattatgatt tagcattagt tggatggaaa ctatcaatta taccagatgc aagtagtatt
     8641 atagcgagta gtggatatac tgatgataag ttaaatggat atatgtctgc acttacaagt
     8701 tctacgtctg aaattgaaac aaaaaaagca tacaaagatg tacagacata tataaaagat
     8761 aatgcaacat ttataagttt agctgtgaga aacaactata ttgtaagtaa tagtagatta
     8821 aaaggtaaaa ttactccaaa tgattttgat gtatatgagg gaatatctaa tttagatata
     8881 aaatcgaatg aatcaaatta aatgattaat ttctatttaa aaagtataaa ctatatgata
     8941 gaataccctt atagggtata aggaggtaat taaatgaaga agaaactatt agtagaaggt
     9001 atgagttgtg gtcattgtgt aaaccatctt aaaactgcat taacagaaga tatagatggt
     9061 attgaagttt tagatgtaga tttagaaaat aagtgtgcat ctgtagatat gaaagatgat
     9121 gtatctattg aaaaattaaa agaagtgata gcagaattag gatttgaact aaagggaatt
     9181 gaatagaaat tgataaacta aaaaaagacc tctattgggg gagtaataga ggtctaaaaa
     9241 tgggggagat tgagtatgtt tgattttaca ttactattat ctcccctttt aaattaatat
     9301 atacataaaa agtaaaaaaa ttttgtaatg actcacattt cttgagcttt ttgaatatac
     9361 tataaatagc taagggaggg agtatcatgg aatctagggg atataaagag gatttaaaaa
     9421 aaatgatact tgatatggcc cacaaagaac ttgagagtta tgtatcacaa aaaggagcaa
     9481 aaaaatactt tgaaggtaac ttggataaga ttattaataa taatataaaa aaattcaatc
     9541 aaagtattgg tcttaataga gtatatttaa aaatgttaaa aaagtgtgca tatcaagaaa
     9601 gtgtaacttc aatatcaaga actataaaga aagaagcatt atttaaatca aaaaaagagc
     9661 tatttgcttt ttcagagtac ttaaaacttg atattaacaa gcgactttca tataatcaaa
     9721 ttttaagaaa aatatcaaga tatatatact acaatagaag ttcatatgct caaaaatacg
     9781 ttgttttcaa aagaggagat gaagaatatc ttttagaacc agagaaaata aaagatgaat
     9841 taatatcaag ctataggtca aagactagag aagatatgag gtcaatagca aaacttttgg
     9901 atatagaagt agaagatgat tataatgctg aagatattag aaaaaaagta ataaattata
     9961 ttattaaaga aaaacttaaa agttagagtt ttaaagaata agttctgcca atttgcatga
    10021 ttaagtataa cttgggtata aattttttat gcatttgaaa gagcttattc tttttttgac
    10081 atattctttt aaataattta ccatgatttg gtaattggtc atcatcattc aaatttttaa
    10141 cttaatatga tttaaataaa tttataataa tgatataata taaaaaaaat aaaaattgga
    10201 gagtgatttc aaaatgaagt ttaatttttg tcataacaat ttcaatgtta ctaatttaga
    10261 aaaaagttta gatttttata aaaaagcttt agggcttaaa gaggtaaagc gcaaggaagc
    10321 agaagatgga agttttatat tagtttattt aggagatggc attactagtc atacattaga
    10381 attaacttgg cttagagatt gggatagacc atataattta ggagacaatg aatttcatct
    10441 ggctttagaa gttgaggagt ttgatgaggc aaaaaaactt cataaagatt tagattgcat
    10501 atgttttgaa aatgaaagta tgggaattta ttttatagcg gacccagata attattggat
    10561 tgaaatactt cctaaaaatc actaatttta aggatatgtt tataatttct ttacaattaa
    10621 taagaaaata agttatcatt aaattgtagg gactaaaaat ggataataat gttatgaatt
    10681 tatgactata aaaatttgtt ggggggagtg atagtagtga aaaaagctat agcagcttta
    10741 ggaattgggg cagtagcagt atctgttagc tccataaatg caagtgctct tgaaaaggga
    10801 acagtaacag caagtgcctt gaatataaga agtggaccaa gttctgattg cgataaggta
    10861 gcaaaattat acaagggaaa aactgtagag attttagaaa agtctaatgg atggtataaa
    10921 gtaagggtat cgagttctgt tgttggatgg ggaagtgcaa aatacatatc aacaagtggt
    10981 tcgtctgaag gtacatcaag tcaaaataat ccaacttcaa gtgggacaac tattagtgga
    11041 aatggtaaag taaatgtgag ttctagactg aatgtaagaa gtggtgcagg tactaactat
    11101 tcattagttg gtaaagcaaa taatggagat atagtaaagt tactagaaca aagcaatgga
    11161 tggtataaaa taaaactatc aaatggagtt actggatggg ctagtagcca atatatttct
    11221 aaaacatcag aagatgttgg aacgaacaat tctagtaatt caaatagtac taataattct
    11281 gataaaaaac catctagcga agaatcaatt gaaggcaaga atggtaaggt aacatctgct
    11341 gtgagtttaa atgtaagaag tggtcctggg acaagctatt ctataatagg aaaattaaat
    11401 ggtggagatg ttgttgaatt aaaagctaaa agtaatggtt ggtataaggt aaagttatcg
    11461 agtggaacaa ttggatgggt gagtgcaagt tatatttctg agactaatga aggtacaaaa
    11521 gaaaaaccaa attcatcatc taaccaaaat tctcaaccta atagtaactc aaagccatca
    11581 tttactggaa attcagataa atctacagca aaaggttcaa caatcgtaga ttttgcatat
    11641 actcttattg gaataccata tcaatgggga gcttcagggc ctgataaatt tgactgttct
    11701 ggatttacac agtatgtttt taaacattct gtaggcgttt ctattcctag agtttctaga
    11761 gaacaggcta attttggaag tgcaatttct atggggaatt atgcaccagg agatttagtt
    11821 tattttgaca ctgatggtga tggaacaact aatcatgtag gtatatatgt aggaaatagt
    11881 aaatttatcc attgtagtgg tactcagact aatcctaata aggttaaagt ggataattta
    11941 actagttcat attggtctaa agtattactt ggtgcaagaa gatttgttta gtaagtacaa
    12001 tttcttatta aaaaattaga agaaataatt atttatattc ataaattgtg actcttaaaa
    12061 atatacttta ttaataatat aattttggga gggacaaact aatgaataga agaaaaacga
    12121 tatacagagg atttaataat aatagacata agattgattt acgtaaatat gttataacga
    12181 ttgcttgctt gtgtctaatt ggctattatt cttatacaaa aataaaagat agcaagatat
    12241 tggagtatgt atcggctaaa ataccatttc taaataattc atcagatata acttataaag
    12301 atatatctga tgaattaaat tcaataaaaa aagggaaaaa atctaaatca caaaccaatt
    12361 cagatgataa acaagaaact aatccagaaa aagcggtaaa taacactaaa gagccagaag
    12421 aagtcaaatt agctactata gaaggctggg atatgtatac tattcaagtt gctgcaatag
    12481 ataataatga tgatttgaaa aaaatacaga catcattagt taataatgac ataccttttt
    12541 cagttatgga gaaggatgga gtaaaaaaaa tacagaccta ttcctctttt gatgaaaatg
    12601 atgttagaaa gcagataagc agtgtaagaa aggtgtttcc agatgcattt ttatcacact
    12661 tagatgcacc tatgttgtct ttagagtata caagtaacta cgcttatata gagagtatat
    12721 ctaaagaatt aaataaatta ataacaaact ttaaagaaga atcaagtttt tggtcgaatg
    12781 ctgaaaataa tgtagatatg gaaaaatata atactatttt gacaaataga aaggcaatat
    12841 cacaaaatat atcaaaagaa gctgaaaaga ttgattattc tgaaatgcgt ttatttaaag
    12901 ataatttaat agagtatgta aagaatgtga atgaaaaaat tgatacagct tcaaaagcag
    12961 caaatgaaga aaaatatagt gtaagtaaaa gtctgctttt atcatccatg caggagtact
    13021 ctatgtttat aaattctata aaataaaata cagaaaattt ggactttaga aagctatatg
    13081 aaaaggattg tttaattaag caatcctttt cctcaatttg acttaatata gaggtgttga
    13141 tttataaaat aaattataaa aatacggata tttttttaac aattgttaag attttttaat
    13201 atatatagta tacttataga aggcagaaaa aatgtttttt tatagataag ggggtaaaac
    13261 aatgaaactc ttaactttta agggagggat acatcctcca tatagaaaag agtactctaa
    13321 cacaaaggca cttgaaaaag ctcaagctcc aaagatagtt tacattcctc ttcagcaaca
    13381 tataggggca ccagctaaac ctattgttga agttggtgat gaagtaaagt ttgggcaaaa
    13441 aataggtgaa caacaaggtt ttgtttcatg taacgttcat tcttcagtat caggtaaagt
    13501 tattgctatt gaacaacatg aagtaccagg aggttcagct caatgtgtag ttatcgaaaa
    13561 tgactttaag gaagaattac atgagagtgt tcaaccaaaa ggtcagttag aggatttgag
    13621 caaagaagat atagtaggta tcataaaaga ggcaggaata gtaggtatgg gtggagctac
    13681 attccctaac catgtaaaag tatctcctcc accagatagc aaggcagaag tagttatatt
    13741 aaatggtgca gaatgtgaac catacttaac tgcagaccac agattaatgg ttgaaaatcc
    13801 agaagatgtt gtgtttggtt taagagcatt aatgaaggta ttagatgtca aaaaaggatt
    13861 tataggaatt gaaacgaata aaccagatgc tatagaagca attcaaaatg tagctaaaga
    13921 ctatagcgaa atcgaagttg ttggccttca agtgaaatat ccacaaggag cagagaaaca
    13981 acttatttat gcgtgtacag gcaaagaagt tccatctggt ggattaccaa tagcagcagg
    14041 tgcagttgtt gacaacgttg ccacagcagc acagatagct aagtctatta aaacaggtat
    14101 gcctttggtt gaaagaatca ctacaataac aggtagttgc atcaaagagc caaagaacct
    14161 aataacaaaa gttggaacat tagtttcaga aataatagaa caatgtggtg gatttaaaga
    14221 agataagaag attggtaagg ttataatggg tggacctatg atgggaatag ctcaatatac
    14281 tactgaaata gcgactaaca aaggttcttc aggaatttta tgtttggatg aggaagaatc
    14341 acgtactcca gatattcaaa actgtttaag atgtggaaga tgtacagatg tatgtccatc
    14401 attcttacaa ccacttttca taagtgcata ttctttaaaa gatgactatg acacagctga
    14461 atatcataga gcaatggatt gtatagaatg tgggtcttgc tcatttattt gtccagcaag
    14521 aagaccatta cttcaatcta taagatcagc aaagagagaa ataggagcaa agagaagaaa
    14581 gcaagctgct cagaaataaa gaaagttaag ggggaaaaac gatggaaaat aagttgatag
    14641 tatcatcttc tcctcatgtg agaagtaatg aagatacttc atatataatg aaacaagtta
    14701 ttatagcact ccttccagca gcagtagcag gagtatactt ctttagactt aatgctttga
    14761 gtgctatgtt tttttgtata cttggtacag taggtactga atttttatat caaaaattta
    14821 cgaagcaaaa aagtactata ggagattttt cagctgttgt aacaggatta ttattagcat
    14881 ttaacgtacc agcatcactt ccttggtgga tgtgtctagt aggaggaata tttgcaatat
    14941 tagtagttaa aatggtattt ggtggaattg gatgtaactt tgtcaatcca gcacttgctg
    15001 caagagcatt tttattagca tcattcccag tagcaatgac tgcttggact caaccaggtg
    15061 ttaactggat aggtaaaaat ttagatgcag ttactacagc aactccatta agctttttga
    15121 aaaatggagc agcaggactg gcagatcttt ctagcaatgg aattagtctt gctgacatga
    15181 tgatagggaa tattggtgga tgtataggtg agacatcagc aatattatta ttattaggtg
    15241 gagtatacct aatgtataaa ggcataataa attatgttat accagtattt tacatagcaa
    15301 ctgtatttat attaacattc cttttaggtg gatttaatat aactttcgca atatatcaac
    15361 tgtttgctgg aggacttatg ttaggtggat tcttcatgct tacggattat acaacttcgc
    15421 ctatgactaa aaaaggtcaa ataatatacg ctgtattagc tggtcttata acaactgtta
    15481 taagaatgta tggtggatat ccagaaggtg tatcttactc aatactatta gtgaactgtc
    15541 ttgctccact tatagataag tttgttagaa atagagtatt tggggaggtg gctaaataat
    15601 gaacagtatg gtgagattag gtggtacttt acttgctata agtgcaatag ctgcattagc
    15661 tcttggagct actaatcagg ttacagctcc agtaatagaa caaagaaata tacaagcaaa
    15721 taatgaactt agaaaagcag tccttccaga agcaaaagaa tttaaagaaa tgaaggaaga
    15781 tacttacaaa ggccttggag atggtcttat agctgaagtg tatgaaggtt tagatggttc
    15841 tgaagtggtt ggatatactt taaaagctaa accaagtgga tatggtggag aaattgaagt
    15901 tatggtaggt atatcatctg aaggtcaagt tacaggtgtt gacattggta atatgtcaga
    15961 aacagcaggt cttggagcaa aagctaagga tgatgcattt aagggtcaat acaaaggaaa
    16021 aactgctgag cctcttgaag tagcaaaagg aagtacaaca gcagataatc aaatattagc
    16081 aatatcaggt gcaactatta catctacagc agttactaca ggagtaaatg ctgctataga
    16141 tgttttcaat agtgctctta ataaataagg agggttaatt atgaatttag ctaaagtatt
    16201 taaaaatggg ctaatagatg agaacccaac atttgttcaa gttataggta tgtgtccaac
    16261 actagccgtt acaacttcag caataaatgg aattggtatg ggtctttcaa cagcagcagt
    16321 tttaatatgt gcaaatttag ttatatcttt aataagaaaa attacaccag ataagataag
    16381 aatacctata tttatagtag ttatagctac atttgttaca atagtaggta tgcttttaaa
    16441 agcatatgtt cctgctcttg ataaggctct tggtatatat ataccattaa tagttgttaa
    16501 ctgtttaata ttagctcgtg cagaaagttt tgctttcaaa acaggagcta tgccttcaat
    16561 agttgatggt gtaggtcaag gtcttggatt tactgttgca cttacaataa taggtgcagt
    16621 aagagaatta cttggaaatg gtagtttatt tggaatgaca ttatttggag catctttcca
    16681 accagtatta atatttatat taccaccagg tgcattctta acattaggat tcttatttgc
    16741 aggatttaat aaattaagaa gtaaaaaagc atagtggagg tgtaataaat gaatttaata
    16801 cttttatttt taagtatcgt tcttgttaat aacgttataa catctcaatt cttgggtata
    16861 tgtcctttct tgggagtttc taagaaggtt gatacagcag tagggatggg agttgcagtt
    16921 acgttcgttt taactctagc ttcaattata acttatttta tacagatttt attagtaaaa
    16981 actggaactg gattcttaca aacaatagct tttatattgg ttatagcttc tatagttcag
    17041 ttcgttgaaa tggtaattca aaagatgagt ccatctttat atcaagcatt aggagtttat
    17101 ttacctctta taactacaaa ctgtgcagtt cttggtatag ctctagttaa tgttcaaaaa
    17161 agttataatt tagtagaaac aataataaac ggatttggag ccggagctgg atttacttta
    17221 gcaatagtaa tattcgcagg tataagagaa agactagaat tagctgatat accagaagca
    17281 tttaaaggtt tccctataac gcttatatca gcaggtttaa tgtcaatagc tttcttagga
    17341 tttacaggac ttataaagct ataaaaaaga ggggtgaaaa tggtgatact tacagctgta
    17401 ttagttttag gaattatggg cttaatcttc gggatagtac tagattttgc atctaaaaag
    17461 tttgctgtag aagtagacga gagagtggaa gctatactag gagtacttcc aggagccaac
    17521 tgtggaggtt gtggatttcc aggttgtgga ggtcttgcaa atgcgatagt tgaaggcaat
    17581 gcaccagtta atggttgccc tgtaggtgga gcagatgttg gtgcaaaagt tggagaaata
    17641 atgggaataa gtgctgaagc aggtgagaaa caagtagcaa aagttatttg taaaggaact
    17701 tgttcaagtg caaaagataa atatgagtat gaaggaatat cagattgtag agcagcaaat
    17761 gttttaaact caggtgctaa gatgtgtaaa tttggatgtt taggtcttgg aacatgtaaa
    17821 gatgcttgta aatttgatgc tatttctata gttgatggaa tagcagtaat agatgaagaa
    17881 aagtgtgtaa attgtggaaa atgtaaagaa gtttgtccta agggtataat aatcacaaaa
    17941 ccagaaagtc aagaagtagt tgtagagtgt aatagcaaag agtttggtaa agctgttaaa
    18001 gaaaaatgta ctgctggatg tattggctgt ggaatgtgtg taaaagcttg taaatttgat
    18061 gcaataatat ttgaagacaa aatagctaag atagacccta ataagtgtgt aggttgtatg
    18121 caatgtgtag caaaatgccc tacaaaagta atatcaggtg acataactaa gaaaaagaaa
    18181 gttactattg accaagaact ttgtgttgga tgtacagtgt gcaaaaaaca atgtaagttt
    18241 gatgcaatag agggagaatt aaaagaaaaa cataaggtag atgcagataa atgtgtagga
    18301 tgccatttat gtatggaaaa atgtccaaag aaagctatta aaattttgta atatggacaa
    18361 aataaggata tggaataatt tccatatctc taatgagtac ataaaacaga ggtatggaat
    18421 aatttccata cctctatttt atgtatattt attctaatgg taataaatat aacaatagtg
    18481 taacaaaaaa ggttcgacat ttctgttgaa ttttttacaa tatagtgata gactaaatag
    18541 gtacaaatta cgtacaaatg aggtgaataa atgaatataa tcttggcatc agcctctcca
    18601 agaaggaaag aaattctaga gaatacgaat gtaagatttg atataattaa aaatgaaatt
    18661 gatgagatta tattggaagg tgaaacacca aaacatttag ttatgagatt agcttttgaa
    18721 aaaagtatgt ctgtagcatc tgaacataat gaagatgttg ttattggagc tgatacagtt
    18781 gttgttttag acaatgctat tttaggaaaa ccaaaagatg agtcttgtgc aagagatatg
    18841 ttaaaaagac tatctggaag agagcatcaa gtcataacag gtattagctt aataaacttg
    18901 tgcgaagata aaaaggtaat tgactatgta atcagcaatg tgaaatttaa aacgttatca
    18961 gagcaggata ttgaggatta tctgaaaact aatgagtcat ttgacaaagc aggagcctat
    19021 ggtatacagg gttatggagc tttgttagtt gaagaaatta gaggagatta ttttaatata
    19081 gttggtcttc caatttcaag gttaggcgac ctattaaaaa aatattttag tataaatctt
    19141 ttttatggag tgtgatttat ctgaaaaagt catttaattc aaccattaag gtaaaagaaa
    19201 tggcaccaga ggaaagaccc agagaaaaga tgcttgccaa aggcgtaaaa agcttatcaa
    19261 atgctgagtt gttggctata cttttaagaa ctggcaacaa gaataaaaat gctattgaat
    19321 tagccaacta tataataaat agagatattc agggtattag acatttagaa gatatgacaa
    19381 tagaggagct gtgtaatata gatggaattg ggttatcaaa atcaacccaa attaaagcag
    19441 ctttagaact aggctctaga gtagctagtt ttaaacctat aaagtataaa ataaggaatc
    19501 cttgggatat acagaggtat tatatggata gcctaaggta tttaaaaaaa gaggttttta
    19561 aagcggttct tttaaacaca aaaaatgaga taatatctga tgtagatgta tctataggga
    19621 ctttaagctc gtcattagtc catccaagag aggtttttaa agaagctata agaagaagtg
    19681 caagtaagat aatagtgatg cataatcacc cttcaggaag tgtagaacca tcaagagaag
    19741 ataaaaatat cacatcaaga cttattaaat gtggagaaat aattggaata gaaataatag
    19801 accatataat tattggagct ggattatact ttagctttaa agaaaatatg ataatttgac
    19861 tttatatgtg ataaaataat aggagaatgt tttaaacatg attcttaata gtaggaagga
    19921 gtaaaatcat ggctaaggaa aaaaagaaag aaaaaaaagg tttttttagt tttaataaga
    19981 tgacaaagga tatgggaata gatttaggga ctgcaaatac attagtttac ataaaaggac
    20041 aaggtattgt tgttagagaa ccatcagttg tcgcaataag agatgatagt aaagaggtat
    20101 tagcagtagg tgaagaagcc aaaaaaatga taggtagaac accaggaaat atagtagcta
    20161 taagacctat gaaagatgga gtaatagctg attttgacat aactcaatca atgattagct
    20221 attttataca aaaagcggct gataaaaaag gtgttgtaag tccaagaata gctatatgtg
    20281 ttccttttgg agttacagaa gtagaaaaga gagctataga agaggctgca agacaagcag
    20341 gagctaaaga tgcattcctt atagaagagc caatggcagc ggctataggg gctggattaa
    20401 aagttgaaga accagaaggt aatatggtag ttgatattgg tggaggtact tcagaaatag
    20461 ctgtaatatc tttaggagga atcgttactg ctaaatctat aagaataggt ggagacgaat
    20521 ttgacgagtc tatagtggct tatgtaaaaa aagaatataa tcttatgata ggtgaaagaa
    20581 ctgctgaaaa tgtaaaaatt aacataggtt ctacatttaa agatgatgaa gaaataaata
    20641 tgcaaataag aggtagagat ttaatctcag gattacctaa aacaatagaa atatgctcta
    20701 cagaagttag agaagcatta aaagaacctg taagttcaat agtagatgca ataaaatcta
    20761 cactagaaag aactccacca gaattagcct cagatataat ggaaaacgga ataatgctaa
    20821 caggaggagg cgctttgctt agaggtcttg ataagcttat tacacaggaa actggtatgt
    20881 ctgtacaaat agcagaaaca cctcttgatt gtgttgcact tggaacaggt aaatctgttg
    20941 aagaccaaga aatctttgaa aaagtattaa tgatgaatac taagaattag aagttggtga
    21001 tggccttgag atttgacaag aacaaaaatg agaaaaaaag gattaatgtt aaagtgatag
    21061 caacgggcgt agttgctatc actttaattg gaattgtggg tatatcaatt ggtaaatttt
    21121 ctagtggtag tccagtaaat ttaggagtag cttcagatgc tattacaagt gtaggtaagg
    21181 gaataaatga tggattttct tttatcaaaa atggttttaa agatgtagca aattttaaag
    21241 ataattctaa aaaggtaaaa aaattagaag aagaaaatga aaaacttaaa aaaaatatga
    21301 tagctttaaa tgccaaacta gataaaacag aatcgctaga agagttaaaa aagacactga
    21361 actttgtaca agaagagtat aaggcgacaa gtatttcagc aagtgttgtt ggtaaaaatg
    21421 atggaaactg gtatgagagt tttgtcatag gtgcaggaaa aaattcaggt gtaaaaaaag
    21481 aaagtatagt tatgaatggt agtggattag ttggcatagt ttatgaggtt tccaacaatt
    21541 atagtaaggc aatttcacta ttggattcta aggcatctgt aagctttaaa ctagcaaagg
    21601 atgccaatgc taaaggtaca attactcaaa atactacttt agataataaa gatagttata
    21661 atagtaaagg atatttgcaa ggatatatgt ttgactcatc atataatgta attcaaggag
    21721 atataataac aacatcaggt cttggattct tcccagatgg tataccaatt ggcgaagttg
    21781 aaaaagtggt tgatgataaa gataagtcat taaaatatgt agttgtaaaa ccttatgttg
    21841 attttaagaa tataaatgac gttgtagtca tagaaccaag aaatatagga taaaggggat
    21901 aaaaacaatg aaaaaagttt tactttgtct gttggggatt gtgcttatga ctatagaaaa
    21961 tagtataaca aattatatag atatttttgg cataagcttt aatttagttc ttatatatgt
    22021 aaccataatc tctctttatt tagatgaatt agaaggtggg attatagcag caataatagg
    22081 gcttattaaa gacataaccg ttggtggaat ttttggtgta aatgcattaa ttttgtttat
    22141 tattgcatat gcaattggat acatgagaga tcagctatat aaaggtagtt atattattac
    22201 tttcgtttta atttttatag gcagtttaat tgattcaatt gtcaatatag gtacatctag
    22261 tataatttat caaagctaca acatatcaac actttttgta aaaggtctac tagtagcacc
    22321 aatagtgaat agtttaattg gtttagtaat atatcgctta tctaaaagag cagttttaaa
    22381 acttaaagaa gattagttgg tgatttcatg gaagaaaata aacaagttga tagactaaaa
    22441 ataattaaga tagtcatggg cgtgatattt ttagctattt tagtaaaaat aatctatatg
    22501 actacattta aatatgaata ttacaatgag ttagctgaga ataaaacata taaaaagttg
    22561 gcaatagaag ctccaagagg agaaataaaa gatagatatg gaagactgtt ggcaggaaat
    22621 aaaaacttat ttactgttca agtttcaggt aacgatatta ataagaaaga tgcaaataag
    22681 cattcaagag caaatgaaat atcattaaaa cttataaatt tgctggagag aaatggtgaa
    22741 gaatatgtag atgagtttcc tatatatgta gaaaatggaa agtactatta tacatatgac
    22801 agagatatta gggaatataa atcagaaaat ggtattccaa atgattataa tgctaaggaa
    22861 agtttttatt acttggtgga taaacttata tcagctggaa tattatctca agaagataaa
    22921 agattggatg cgacaagatt acaggcaaaa ctaaatgaaa atggatatta tccacctatt
    22981 ttagttagta aatggatgtt cacagcagaa agagataaga gagattggtt ggctagttat
    23041 aagataaaag aaactaaact gagtgccaaa gaagcgtttg aaaaagtaag aaatagtgat
    23101 gcattagaaa ttgataaaag tttgagtgat gaagatgcaa gaaaaataat ggtcgtaagg
    23161 gatttgataa aatcaaaagg atattctcaa tataatccag taactattgc taaggatgta
    23221 ggagaaacta cgattgctca aattgaagaa agtgctatgg accttgttgg tgtttcaata
    23281 gcagtagaac cagtaagata ttatcctaat gggtcattag cttctcatat gttaggatat
    23341 gtaggaaaaa tgccatccac tcaaatagaa tcataccttc aaaaaggtta tgaaactgga
    23401 gacatggtag gtcttgcagg agtagaaaag agtaatgaaa gtagattaag aggaactgat
    23461 ggttataaaa tggttaaagt tgatgcattg ggtagaatca gtaaagaaat tgaatctaaa
    23521 aaacctaaat caggagatac tgtgtatctt acactagata aagatttaca agaagtttca
    23581 gacaatgcat taaaacagat aatcgaagtt gcttctaaag gaggtacttt taaaagtaaa
    23641 tttggggaca aaccaattag tgcatatgca ggaaaagcac agtcagcagc tcttatagct
    23701 attgatgtta aaaatggaga agtattagct tcatcaagtt atcctaatta tgacccaaat
    23761 aaatttgcaa aaggtatttc tacagaggat tataaagcac ttcaaccaaa aaatccaaat
    23821 gacttactag caggaagtcc acttttaaac ttggttacac aaggggaatt tcagccaggt
    23881 tcttcattta agatgctaac ttcaatggca gcgttagaaa atggtctgga tccaaacttt
    23941 actataaatg accctggggt tataatgtta ggtaaaaaat cttttggtga ctatgtatgg
    24001 aaccatggta gaggaaacca tggtatgaca aacttatata aagcaataca agagtcttgt
    24061 aatatatata tggcaacaat tggtacaggt aaaacatggc cagatggaaa aagcattggt
    24121 atagatatga atgcaaataa aatacttgag tatgcaaaat tatttgggct tgaccaaaat
    24181 acagggcttc aagatgaagt agaagaaaga gcaggtaaag ttcctagcac agaagataaa
    24241 ttgaaatcaa cacaagctct tctaaagtca aatttagaaa gagaaatggc aaatgatttt
    24301 gttgatataa ctagagaaaa aaatcctaaa gaatatgaga aaagaatcaa tgagattgta
    24361 agctgggcag cagaaaagaa aactcctggt agagttgaaa caatgaatag gctaaaaaaa
    24421 ttggatgtta aagaagatag aattgaagaa gtagcagatt tagcagtatt tagttatttt
    24481 aactttgcaa aatggagtac tgcagataca tttaacttag ctataggtca aggtgaaaat
    24541 gcttatacac cagctcaaat atcaagatat gtagctgcta tagcaaatgg aggtaattta
    24601 gtagaattat ctgtagttga tagagctgtt tcaagtgatt attcatcagt taaaattaat
    24661 gaccagaaga aagtcgaaaa aatacctttt aaaaatccag ataacttaaa agaattaaca
    24721 aaaggtatga aattggttgc aagacaaggt acagcgaaga gtgcttttgc tgattttcca
    24781 atagatgtag cagcaaaaac tggtacagct gagaaaagtg ggaaaatacc tacagataac
    24841 gagtatgaat atttaaaaag tcatatgtca tcttataatg tcaacctaaa tgatgcgatt
    24901 aaactagctg ataagatgaa agcagaaaaa gaaaaagagc tttcattagc taaggaaaag
    24961 gaaataaaaa agaaattaga aaataaagat ttgaaagacg aagaaagaaa gaaattagag
    25021 gaagaattag aagatggagt aaaagttaga ttagaggaca ctgataaggt gaattcttca
    25081 tatctaagaa aagctataaa agaattaaat ccaaaaataa cagatgacca aatagataga
    25141 tttaaacaag attatggttc ttttacgtgg actgttgctt ttgctccagc agatgaccca
    25201 gaaatagctg ttgtatgtgt aattcctcaa ggtgattcaa gtgtattctc acttttacca
    25261 actagagaag ttattggaac ttatatggga ttagagccaa ctaatagtaa aaatgacaac
    25321 aagacgaatg atgttaacaa ttcttctgac gaaaatataa atttcgagtc acaaattaat
    25381 agatagctca atctatgtat aaactttagc tttatataga aaaaataaaa gggtttaaag
    25441 cgtatttgaa gaatataaat gatatatcac tctggaggtt gttatgtctt taagagaaat
    25501 ttgtagtcaa gaactagtag agttcaaggg taataaaaga gggataatag taaatataaa
    25561 aagagaagct ccatttgagg aaatacagga aaaaataata aacaaattgg aagcatatgt
    25621 tggctttttt aatggggcta aaattagtaa aattaatagt gattgtttaa ctgacatgga
    25681 gattctagaa cttaaagaag gaattacttc tagatttgat gtggaatttg ttgaagatca
    25741 aaaaattgaa gaaaatagta attttccaac aaaatatgtc aataccctaa gatctggtga
    25801 aaatattgaa tttgaaggtg atgtagttat attgaatgat atgaaacctg gttcaaaagt
    25861 cctttcaaaa tcaaatacag ttgttatggg agatataaat gctggtgcta aggttgtagc
    25921 aggaggtaat gtttttgtaa tgggtaaaat agaaggtttt gtacatgcag gtgcagaagg
    25981 caatgaattt gcatatgttg ttgctggaaa tcttaatcct aaaatattac aaatagcaga
    26041 taatattgca gaagctccag atgatgaaga aaattatgaa agtgaatcag aaattagccc
    26101 agaaatagcc tttgtaagta atggaagaat tgtaatcgaa agctatttgt caaagttaga
    26161 caaatagaaa gaaaaatagg gggtagtatt atgagcgaag ttatagttat aacatctggt
    26221 aaaggtgggg ttggaaaaac tactaccgca gcaaacttag gaactgcgct aagcctagaa
    26281 aataaaaaaa cagtagttgt ggatgcagat attggactta gaaacttaga tgtagtaatg
    26341 ggtcttgaaa atcgaatagt ttatgacata gtagatgtag ttgaaggaac ttgtaggctt
    26401 aagcaagctc taataaaaga caaaagattt gataatctat atttattacc agcagcacaa
    26461 actagagata aaaatgctgt ctcagttgag cagatgattg acctgtgtga gaaactaaaa
    26521 gaatctttcg agtatataat aatagactgt ccagcaggta ttgagcaagg ctttaaaaat
    26581 gcagtagctg gagcagatag agctattgta gttactaatc cagaaatatc agcagtaaga
    26641 gatgctgata gaataatagg tctattagaa gcaaatgaaa taaaagagat aagattagtt
    26701 ataaatagaa ttagaaatga tatggttaag cgtggagaca tgatggataa acaagatata
    26761 atagaaatat tagcaataga tttgttaggt cttgttcctg atgatgaaag cataattata
    26821 tcaacaaata aaggagagcc agctatactt gattctaagt cacttgctgg tcaagcatac
    26881 aaaaatatcg caaaaaggat actaaatgaa gaagttcctc tacttgatct tgaagttgaa
    26941 gatggattct ttggtagact taaaaaaatg tttagcatgg ctaagtagca aaggaggata
    27001 catctgtgtt agatttattt agagtttttt ctaacgaggc taaaactagt aaatctgttg
    27061 ctaaagagag gttaaagcta gttttagttc atgatagagt agattgttca ccacaacttt
    27121 tagagatgat aaaaacagac attttgaaag taatagctaa ttatgcagaa atagaagatg
    27181 atggtcttga aataaaaatg tctaaatgca gaggtgagca tgatgacaaa cctgtatctg
    27241 cattagttgc taatatacca ctaaagaata taaaagatag atgtatgtaa ttagtgctta
    27301 aaagggggat atccttattg gtatatgccc cttttattta attatatgtt taaagataga
    27361 aagacggtga ttctttgaaa aaaatcaata ttaattataa gtcgacaatt aagttgataa
    27421 aacagttaga ctggaaactt atagttacag tattagctat ctttattttt gggcttgtta
    27481 tacttagtag tgcaacacat gctaattcaa caggttcata taatcaattg attaaacaag
    27541 gattagcttt tgtacttggt ataggaatga ttatagtcat attatttttt gactataacc
    27601 ttctaggcag gtattataaa gcgctttata taataagtct tattttgtta gccatagttt
    27661 tattacctgg aattgggaca gtaaaaggtg gagctaggtc gtggattaac ttaggaccac
    27721 ttgacttgca gacatctgaa atagtcaaat taacatttgt actaagttat gcaaagatac
    27781 ttgaatccaa aaaggataaa ttaaatacgt taaaggaagt tatgcctgtt gtagtttatt
    27841 ctcttccatt tatcggatta ttgattgcac aaccagactt gggaacagga attgtatttt
    27901 gttgtatgat atttgcaatg ctgttcacag caggtcttag ctcaaagttg ataaaaagag
    27961 gaataatcat actattagtg tcaatgccac tgatgtattt aatgatggca gaccatcaaa
    28021 aggtaagaat agaagctttt ctaaatccag aagatgtaac tctaaaagga aactatcaag
    28081 ttatgcaatc tctgattgca attggttctg gaggcgtaac tggtaaagga ctttacaatg
    28141 gaagtcaaaa tcaagaagat tttttacctg tacaagatag tgattttata tttgctgttg
    28201 taggtgaaga acttggtgta ataggaatgg cagtgttgat aatcttattt atgatattcc
    28261 tattaaggct tttagcaatt gcaagagatg caaaagattt ttatggaacc ctcatagtgg
    28321 ttggtgttat gggaatgttt ggttaccaga taatccaaaa tataggtatg acggttgcgt
    28381 taataccagt aactggagta acattacctt ttgtaagtta tggaggaagt tcgttattga
    28441 catctttagc aaacttagga ttagtactaa atgtgtgtat gagaagaaag aaaataaatt
    28501 tctaaattta aatacatcta aacataaatt gaggagatta gatatatgaa tatagcatta
    28561 gtagcacatg accaaatgaa aaacactatg gtcggatttt gtataggtta tgaatcaatt
    28621 ttaaaaaagt atggactata tgcaactgga acgacaggaa aaagaataat ggatgaaact
    28681 gaattaaata taaatagatt agcatctggt cctttaggtg gagaccaaca aataggttct
    28741 ttaatagtaa cacaagaaat agatttagtt atttttttaa gagacccatt gacatctcag
    28801 gctcatgaaa cagatataca agcattaata agactttgtg atgtttatca tgttccaata
    28861 gctacaaatc tagcttcagc agaaatattt ataaaagctc ttgatagagg agaactatca
    28921 tggagagaag ttagaaaaag caaaagtcaa cgtatttaaa aaatattaat attacagtct
    28981 aaagaaacag atatataagt gtttaatgcc ctagtctttc tgttgcaata aacctagtac
    29041 ctgtagaaat cttatgaaag atttaaatga tgaagagcta aatttgagat taaataatta
    29101 ggaaatagat tatagataga gtagtagaat ttaaactatt actctatttt aataatatga
    29161 aatagaatca agtctattat tatatatatt taaacttata ggtaatacta tttcaaccaa
    29221 gtaaatattg ttatgacaac attaataaaa caaaaattta tttttaaaat ttgataaata
    29281 gctgtatatt agtatttata gattaaatat taatatacag ctatttatta tttttgagtc
    29341 tattgtagaa tataaacctt gcattataga gtactatgta ttattatagt aaatataagt
    29401 actatacatt atagagtagt aggtgaaaag gcggtggtac ataatgcagt taaataaaga
    29461 agtgttaaaa ggttatatcg atatattaat tgtttctata ttagaaaaga aagattgtta
    29521 tggatatgaa attgcaaagc aagtaagaga aagaagtgag tttgaattaa aagaaggaac
    29581 aatgtatctt gcacttaaaa gaatggaatc taaaaattta attaaatctt attatagcaa
    29641 tgaacaaagt tcaggtggaa gaagaaaata ttataattta actaatgaag gtaaagattt
    29701 tttagaaata aagaaacaag aatggagatt tattaaaaag gttatgaatc aatttttggg
    29761 agaggtggat taatttatga aactaataga tgaatattta gataaactat ataaaaaatg
    29821 tgacaataaa agtactatag aattaaaaca ggaaatgaga tgccatctta tagaatctgc
    29881 aaatgaattt aaattagaag gattagatga agaagaagca tgtaaaaaag caattgaaag
    29941 atttgatgat ggtgatgaaa tgcagtatga attatgtaat ataataaaag aactatcttt
    30001 atcattggat aggcataaat caattgtaat ggggtttaaa aaagtattag gttacataag
    30061 tataattgca tttttaattt ctggatttat gtggtattac aataatagtt tacaacataa
    30121 tatgtataat ttaggaaaag aacttgatgg agaaataaag caattggcag aaagacatga
    30181 tatgacaaag attggtgaat ataagttaga acttgaaaaa atacttgata aagataagta
    30241 tagtaaagtc aaagctctta gactatatgt aatagatatg aaagatggaa acactaattt
    30301 aagttcttct ggattgaatg ctaatatggt atatgagagg gaagcagatt ataataacat
    30361 aagtaatttt atacagcatt taggatataa tggaaaagat tttttagaca agaatgggaa
    30421 tatagtaaat ccagatattt tcttagaata tttcttttat tttgaatcgg agatgttaat
    30481 tccagtagca ttcgtatttg gattattatg tataatagcg tattttatat taagatttaa
    30541 aatttcttta ataaaaaata ataattaaat aattcaccaa ataattttaa tttgtagagt
    30601 aaataagatg tttcaaaata aaatttgaat catcttattt tttattgttt gttatattct
    30661 tttcttttat atttctccat agtggattgt atctatacat aatcacatta aaaacaacac
    30721 aacatatgtt aactggaacg cagcagaggg agccaatact gcttatacat tggttactaa
    30781 aaaaggtgct tgttctggaa tgtgtaggag cttgaaagct ttatgtgatg ctatgggaat
    30841 tgaatgttat tatgtacata gtacaacaaa tgaccatcag tggaatctta ttcgttttga
    30901 ggatggacgt ttgtatcatg tagatattga tgccaacaaa tcagcaggag cagacatcat
    30961 atacaagagt cttactcacc cattaccatt tgacaagaaa gcatatccag ctgttggcga
    31021 agtagtaaag acacagaatc caacgccaac tgttaagaaa cctacgccag ctaagccttc
    31081 tgcgccagtt ggacctggga atcacactat gatagcagat caacaagggg caatacttga
    31141 tgaagtttat ggaaataatc tacgtccaaa aattgaaatg tatgagggag ttccgtgtag
    31201 tttaatcaac tcatcaataa atacaattaa gtttacaagc aaaaattcaa atttagaccc
    31261 atcaaagcat acagttactg taatagggtt tatgaataca ccatatggta tggttgcatt
    31321 agcaccagag agcaaacctg ttagttatcc ttttgagtat gataaggcat tttctttaga
    31381 ttttactggt aagaaccttt ctgcagcaga attatcaaaa aagtgtaaaa aaataatata
    31441 attattaaag tagaaattgg tgaaacaaca tctaattctg gaatagggct agtagcatat
    31501 actagatacg tgaattaata caagtatact agtatacaaa tagaacagac tataaataat
    31561 gcaccctgtg cgaaagggta catttattat tatattagtc attttggaaa ataaaaaagg
    31621 agttgaactt aaaattagat tagaaaaatt aattttgaga caactccatt ttaatttatt
    31681 tttaaatata aaatcattta ttttaaataa tattatgttt gattaatggc tatttaacta
    31741 attttgcaaa ttctgcacca aatgccatac actcttttaa tgtctcttca ctagggaaga
    31801 attttttctt aagagattct acaggcattt tgaatgacat agatttaagt aaagtctcag
    31861 ccatagttat accttcacca ctccaaccaa atgaaccaaa tactcctgct atcttgcctt
    31921 ggttagccat tgggtctata acagagaata aatcccacat aggtttaacc atagttttgt
    31981 ttatagttgg agagcctagt aatattactt ttgacactac taatgtatcg tgcatttctg
    32041 ttaatgtcat atcttctaaa tcatataatt cagcctttgc accttcttta tcaagtcctt
    32101 cttttatctt tttagccatc tctaaagtat tactataagc tgataagtag aatatagaaa
    32161 cttgattttg attagttgta ttaacagctt ctgtagacca ctcaacatat cttttaacag
    32221 cagccattgg gtctttagta agcattggac catgagaagt caatatagta tcaaattcta
    32281 tgtttagtcc taccacctta tctacagcac ttaaaacatg tttagcaaat ggcttaacta
    32341 tacaatcata gtagtgttta gctgatttta gataatcttc actattcact acctctgcat
    32401 caacacttgc aaagtgacaa ccaaatgcat cacaagttaa aagagtcttg tcctcctcaa
    32461 tgtaagtaaa cattgtatca acccaatgta agaatggagc agttataaat ctaagatttc
    32521 ttttaccgat atttaaaatt tcaccatctt ttattacatg acagttaaaa ggtctattta
    32581 tttgaccatc taagtatagt ttagcagctt tagtacaata aacttctata ttaggattta
    32641 tatctaataa gtattttaaa ctacctgcat ggtctggttc tgtgtgatga attacaacat
    32701 agtcaatttt agctatatca gtaacctctg ataaattact taaaaactca tctttaaaat
    32761 ttgcctttac agtgtcaaat aaaaccgttt tttcgtcctt gattaaataa gagttgtaag
    32821 ttgtcccaaa ctcagtttcc attattatgt cgaatacttt taaaccttca tctactactc
    32881 cagtaaagta gatatctttt tttacttcaa aaactttact catttttaac cctcctaaaa
    32941 tttatcatta tataagtaac ataccccatt ttgaggtgaa tgaaacaaaa gatgtcatga
    33001 taagtcatgt aaaacaatag ttttatgttt ataatatatc atactttgta aaaaaattgt
    33061 atttcaataa aaatttaata gaaaatattc ttaaatattt ttagctattc tattttgtga
    33121 ataatatgta ttttaaattc aatattttag ttatattttt gatagaaaat attttatcgt
    33181 gtataatatt acttataaat aagaataaaa ataaagagct tgtttggagg aattttaata
    33241 aatgaataaa gtagacttga aaaaaatctt aaaaaaagta gaaaagcctg ctagatatct
    33301 tggaaatgaa ataaattcaa ttcataaaga tactagtaat agtgaattga taagatatgc
    33361 acattgcttt ccagatttat atgaagttgg tatgagtcac ttagggagtc atatattata
    33421 tgatgtaata aataaggatg aagatgtatt ttgtgagaga gtgtattctc ctgcagttga
    33481 tatggaaaat ataatgagag aaaaaagtat accattattt gcattagagt caagagagcc
    33541 tataacaaac tttgattttg tcacttttac acttcaatat gaactttcat acactaatat
    33601 cttaaatata ttagatttag ccaatatacc aatattaaaa gaagaaagaa ctttggaaga
    33661 cccatttata atggttggtg gtccttgtgc ttataattcc gaaccattag cagattttgt
    33721 agatgtagta atattaggtg aaggtgaaga ggttaattta gaagtagtaa atgagtataa
    33781 agaatggaag aaaaataaaa cgactagaga agatttttta tataaaatat ctagcataga
    33841 aggagtatac ataccaagtt tttatgatgt caagtacaat gaagatggta cagttcaaag
    33901 tgtaactcct aatagagaag ggattactaa aaatcctact aaaaggataa taaaagatgt
    33961 tgagactgta gattatccag aaaaactaat agtaccttat atagatactg ttcatgatag
    34021 aatagtacta gaattattta gaggatgtac tagaggatgt agattctgtc aagcaggtat
    34081 gatatacagg ccaataagag aaaagagtgt taaaagactt aaagaaatat tagataaatt
    34141 agttaagaat actggatatg atgaaatatc attatcatca cttagtacga gtgattatag
    34201 taagttatca gagttgactg attatttggt agatgaatat gcatctaata atattgggat
    34261 ttcgcttcca tcccttagat tagataattt ttcaatggaa attgctgata aaatacaaca
    34321 agtaagaaaa tctggactta cttttgctcc agaagcagga actcaaagac ttagagatgt
    34381 tataaataaa ggtgttacag aagaagattt ggaaaatgca acagaaagag cttttgagat
    34441 ggggtggaat agtgttaagt tgtattttat gattggttta ccaactgaga cttatgaaga
    34501 tttagatggt atagctaaat tggcttataa agttattgat atttatagaa aagtaaatgg
    34561 aggaaagcta aagagaagtt ttagtgttac agtgagtact tctacatttg taccaaagcc
    34621 atttacacct ttccaatggc atggtcaaga tactacagaa gaagttatta ataagcagag
    34681 acatttagta aataaattaa gaaataacaa tataaaatac aattatcatg attcaaagac
    34741 tagtttgatg gaagcagtag tagctagagg cgatagaaga ataggaaaag ttatatatga
    34801 tgcatttaga ctaggtgcta aatttgatgg atgggcagaa cattttaatt tagatatatg
    34861 gaaagaagct atggaaaaga ataatctttc tatagatttt tatgcacata gaaatagaaa
    34921 ctatgaagag gtatttcctt gggaccatat agatgtaggt attagtaaaa aattcttaat
    34981 tagagaagac gaaaatgcta aaaaagaaaa aatcacttct gattgtagac ataactgcaa
    35041 tggttgtgga ataaatatac atgatatagg aagggggctt tgctaatatg agtaagataa
    35101 taagagtaaa atttaaaaag gaaggcgaca tgatatatat atcacatctt gaccttcaga
    35161 gattgttaca aagagcgttt agaagagctg aaataaactt atcacattct caaggattta
    35221 atcctcaccc taaaatgagt tatggcaatg ctttggcttt agggacagaa agtcaaggtg
    35281 agtatgtaga tattgaaata gaagaagatg atttatcagt agaggaattt ttaaataaag
    35341 tatcaattca acttccagat gggatagatt ttatcaaagc aaaagaaatt gatagacaaa
    35401 caccttctct ttcttctgtg atagactatg gagaatattt gtttaatata gatttgaaaa
    35461 gacctatgac gaaagagttt gtaaaaagaa aagtaattga ctttatgaat aataaagaaa
    35521 taatcataac aaaaaagaat aaaaaaggta agatggttga agtagatata agacctatga
    35581 taagaacctt tgatgtttta aatttagaag atgaacatat tactcttact gctactatag
    35641 ctacaggctc aaaaactaat ttaaatacta atatacttat accaaagata ttagagatgt
    35701 ttgaacttga tattgaccca ttagatgttg atatattgag aagagattta tatgtattag
    35761 aagatgggga attagtgact cctatgtaag ctcgagaggt gaagttgtga aaaagatagt
    35821 tattgagtcc ctaatagggt cccagaaaac agcagtactt gaagatgaaa gattaacaga
    35881 actttttgtt gaagacaatt taaataagaa gactgtatcc aatatatatc gtggaatcgt
    35941 aaaaaaagtt ataccaggaa tagaagcttg ttttgtagat attggattta agaaacttgc
    36001 ttatttacaa ttaaaaaagg gaagtactat aaaatctggt caggatattt tggttcagat
    36061 aaataaagaa gaaataggaa ctaaaggcgc aaaattaaac actgagatta gtatttctgg
    36121 aaggtatatt gtctatatac cttccaatga tagaactaca atatcaaata aaattactga
    36181 tgaaaaagaa cgatttagat taaaaaaaat tactaaggca gtaaataagg aaaatctagg
    36241 attaataata aggactgagg ctcaaggttg cactcatgat gaaataaaga aagatataga
    36301 agagttgaaa ttaaagtatg aaaacatttt aaaagaatat aaacttggta taggtccgaa
    36361 attattatat aagtctttag attttgcaac taaatatgta aaagataatg tgaatgatga
    36421 tattgaatct ataattacca atagttatga taagtactct gaacttaagt ctattttaag
    36481 aggtatagat aagacttatg ttgataaact gtgtcttgag gaaaatagag atgtatttga
    36541 tttatacaga atagaatcta aaatagaaaa attattaaat aaaaaagtat ggttaaagag
    36601 tggaggttat ttaataattg aaaaaacaga agctcttacg gttatagatg taaatacggg
    36661 taagtttatt ggaactggta aacttgatga aaccgtttat aaaactaatc ttgaagctgc
    36721 aaaagaaatt gtaagacagc ttagaattag agacatagct gggataataa taatcgattt
    36781 tattgatatg cataaaaaga agcatcaaaa ggagatttta cacatcctag aagaagaatt
    36841 taataaagat aaaagaaaag ctgaagtatt aggtatgaca aaacttggtt tagtagaagt
    36901 tgctaggcgt agagaaaaag aatccattga taagtattat ttaatgtctt gtccatgttg
    36961 tgatggtgaa caaacaataa aatctgtaca ttatatacta gatagtattg aaaaagaaat
    37021 tatgagaata agtgaacata ctgtttacaa aaacataatg gtagaattta atgattttat
    37081 ttttgaacaa ataaaagaat attatatgga tataattgat aaaattggtg aaaaatataa
    37141 tataaagatt tctttaaacg caaatagtac attaaagcac aataaaacca atgttatttt
    37201 cgataaaata gttgacaata aaatgtaaat attttataat taataattgt aatgccgcac
    37261 agtaaaggtt taaatcttca aatgaagtac cttgtactgg cgagtttgag gccgaggagg
    37321 tgtatttaat gtacgctata gtaaagactg gtggaaagca atataaagtt tctgaaggtg
    37381 atgtattatt cgttgaaaaa ttagaagcta acgcaggtga tgttgttact ttaaacgaag
    37441 tattagcttg ctctaaagat ggagaattaa aattaggttc tccagtagta gaaggagcat
    37501 ctgttcaagc taaagttgtt gaacaaggta aagctaagaa agttatagtt ttcaagtata
    37561 aagcaaaaaa agattacaga agaaaacaag gacatcgtca atcatacact aagatagttg
    37621 tagaaaaaat aaacgcttag ttattggtta agttatgata aaagttaaat attattataa
    37681 tgatgatttc ttgttgcaag gtttttgttt aaagggacat gctgattttg ctgaaatagg
    37741 ctatgatata gtatgtgctg cagttacatc taatgctatt gcagttataa attctttaga
    37801 caaattacag aagattgaat ttgaaaaagt agtaggagaa gaaggtcata tagagtgtat
    37861 agtcaaagat acacatgtaa aagacgcaca attattacta aatcactttc aattagcggt
    37921 taaagaaatt aaacgggaat acccaaaaaa tataaaaatt ttaaaaaagt agggggtgac
    37981 tccatatgtt aaacatgaat ttacaattat tagcatctaa aaaaggggta ggttcttcta
    38041 aaaatggtag agattctata tctaaaagat taggtgttaa gagattcgat ggtcaattag
    38101 taactgctgg aagtataata gttagacaaa gaggaactaa aatacatcca ggaactaatg
    38161 ttggaaaagg tagtgatgat actttatttg cattagttga tggtactgtt aaattcgaaa
    38221 gaaaagacaa gaagagaaaa aaagttagta tatatccagt agcaatagct gagtaatata
    38281 cataaaagga gtgttgaggc actccttttt cttataatac tgatgaattt ataatggtat
    38341 ttttggtaat gctaattaaa agtttttaat taagtaggtg aagaatttgt ttatagataa
    38401 ggcaagaata tttgttaagg ctggaaatgg tggaaatggt tcggtagcat ttagaagaga
    38461 aaaatacgtt ccagctggtg gtccagacgg tggagatggt ggtcgtggag caagcatcat
    38521 atttgaagtt gatttggggc ttagaactct aatggacttt aagtaccaga aaaaatatca
    38581 agctcaaaat ggtggcgacg gaagcaaagg aaaaagagct ggtaaaaatg gagaaaattt
    38641 agtattaaaa gtaccagcag gtacagtaat aagagatgag gctacaggtc ttgtattagc
    38701 agatttaaaa aaagaaggag atacagctat agttgcaaaa ggtggtatag gtggtaaagg
    38761 taaccaacac tttgcaaatg cagtaagaca agctccagct tttgcaaaat ctggtacaga
    38821 tggagaagaa agatggataa ctcttgaact taaaatgata gcagatgttg gtcttttagg
    38881 atttccaaat gtaggtaaat caacatttct atcagttgta actaaagcta aacccaaaat
    38941 tgcaaactat cattttacaa cacttacacc aaatctaggt gttgttcaaa ctaagtttgg
    39001 agatagtttt gtacttgcag atatacctgg aattatagag ggtgcttctg aaggcatagg
    39061 attaggtcat gagttcttaa gacatgttga gagaacaaag gtattgatac atattgtaga
    39121 tatatctgga ttagagggaa gagaccctat tgaagatttt gacaagataa atgatgagct
    39181 taaattgtac aatgaaaagc tttcaaagag accacaagtt gtagttgcaa ataaatttga
    39241 tatattagaa gatgagagta agtttgagaa gtttaagagt gaattagaag gcagaggata
    39301 tacagtattt aaaatgtcag cagcaactcg tcaaggcata gatgaagtaa tagcctatgt
    39361 ttctaaaatg ctaaaagaag tagaagatgt ggagctagtt tcagaagaag aaatgtatag
    39421 accagaactt gatattggta ctgaagaaga gttaagtata gatattgaag atggtgtgta
    39481 tgtagttaca ggtaaagcac ttagaagaat aatgtattct gtaaattttg atgatatgga
    39541 atcccttcaa tatttccaaa aagctatgga gagtcaaggt gtttttgata ggcttagaga
    39601 aatgggaata gaagatggag atgtggttaa aatttatgaa ttagaatttg agttttacaa
    39661 ttaatttaaa tatagattat agataattta agtatagaat tatagaggtg agaatatgtt
    39721 aaaaggaaaa caaagggcgt atttaagatc tttggcaaat actttaaagc ctactacaca
    39781 aattggtaaa gaaggtgtta cagagagttt cttagagcaa ttagatggta tgttaagaac
    39841 tagagaatta gtaaaagtta ctatattaga aaatgcaggt cttgatacta aagaaactgc
    39901 taatgcagta tgtgaagctt taagagctga atttgtgcag gctattggtt ttaaatttac
    39961 tatttacaag aggaatattg aagagcctaa aatacttttt ccaggacatg aacaggcgaa
    40021 agcaaagact aaaaataata atgttacaaa aaagggtaaa ccaactaaaa gagctgttag
    40081 ataatagttt atatcctttt agggaaataa gttaaaaaaa tagtgacaat tggaagaaat
    40141 tgtattatac tattaaacat aaatttaaat tagcaatctt atcaagagtg gcagagggac
    40201 tggccctgtg aagcccggca accagtatat ttaattatac attggtgcta aatcctgcag
    40261 catttatgct gaaagatgag tataaacccg tcatatggct tgagtttata ctcaagcttt
    40321 ttttattggt atatttatat atataaataa tttaatataa tcaaaatgat gggggtatgt
    40381 tacagtgaag atagcaatat tagggtatgg aacagtagga agtggattaa ttgatttaat
    40441 agaaaataat acttcaaaaa gagatataga agttgttgga attttagtta aaaataaaga
    40501 aaaacacaag tataagaagt attttgataa gataactact gatatagaag atatatttga
    40561 caaggatatt gatatactag ttgaagtaat tggaggatta aatccagctt ttgattatgt
    40621 aacaagagcg ttaaataaaa aaatacatgt agtgacagct aataaagatt tactggctga
    40681 aaaaggtagt gaccttatag aattagctaa cttaaataat gttagcatca agtttgaagc
    40741 atctgtggca ggtggtattc cagttttaaa accattgata gaatcactgg aaggcaacaa
    40801 tataaagagc ataaacgcta ttttaaatgg gacttgtaat tttatattat caaagatgta
    40861 tgatgaaaat ttaccgtatg atgtcgcttt aaaacaggca caagagttgg gatttgcaga
    40921 agttaatcca gatgcagatg ttttaggata tgatagtgct agaaagttat ctatattgtc
    40981 tactttatct tatggaaaaa tagtatattg gaaagattta ttattagaag gaataacttc
    41041 aatagatgaa aaagatatag aatatgcaaa aaaattaaat tgtaagataa aattggttgc
    41101 tagaagtaag tatgaatcag gagaagtaag tggatttgtt agaccagcat tagtggacaa
    41161 taataatatg ctttcaaaga tagataatga gttcaacgta gttattttag taggtgattc
    41221 agtaggtgaa ctttcttttg taggtaaggg agctggaaga ggtgcaacag gaagtgctgt
    41281 ttatgcagat attatagata taatagataa tagaagttca aatataaagt ctttttcaaa
    41341 agggaaattg gatttaagtg gacttataga agatgagtgt agtgcagtag ttagatttgg
    41401 tgggtataat aataaagatg gaattttaga atgtttagat aaatatgtag atagttatga
    41461 tattatagat gatgaagaat tggcgatttt tgtaagtgca aagtcagaac atgagattga
    41521 tagagttttg gaaattgtaa aaagaaataa ttacagtgaa tcagctaaaa aattattgaa
    41581 gatagattaa tatgtttatg caagaattat gaaaatttaa tagggtgtct caaaatagag
    41641 agataagcct tttttccagt gttgaaataa taaaaatata tttgatttta taaaaaaaga
    41701 gtgtctcatg aactaaaaag ttcattttga gacactcttt tttaatatct ggtgaattaa
    41761 taagataata gaaaaatgta tacgttataa ttaaccaata aagaaatttt cactagcttc
    41821 attaatagct tgctgagtta cccttatata tcgctgtgta gtggctacag agctgtgatt
    41881 taaaagttct ttaaccaact ctaaattatt gttactcttt tcaaattgag tagtagcata
    41941 gagctttcta aagctatgtg tacttatatt atacagattt aagtatttac aaattatcct
    42001 caactgtttt tgaatagcct ttgtctttat atttactaag tagtcatttg atttaagatt
    42061 atactttctt gcatgttcat ttatggactc aattatattt ttattaactg gtctatactg
    42121 taatttgccc gtcttttttt catttatttc caacatatta cctttaaaat tacctatctt
    42181 taatcttaaa atatctgata ttcttagacc taaattagct tctaacatta gtgccaatgc
    42241 aactttagga ttttttctaa atcttttttc aacgccattt tcactataag taaagccagt
    42301 atgtaatagt tccattatct ttttatattc gtctatttct ataggtcttg ttgccatttt
    42361 caaccactcc tcttagtttg atttggtaag tttaggtcgg tatttattat ataatataaa
    42421 tgaaatatta tataatggaa aaatttccca gtaaacttat acaagtgtga tatatgtgat
    42481 taaggagaat tttttgaaaa atatttctaa taaaattatt aaattgtgat gaaataacta
    42541 tgctgttaaa ctaattaaaa aggttattaa aattatgaca aatataataa aaaaatggaa
    42601 gtatttagtt aagttttatt agttttattt ttagttttat ttttttcgtg aaaatttctt
    42661 agtgaaaaaa gtatttttat atcgcattag tttcccattt gtagataaag ttttcatatg
    42721 tttttttgca ataaatacgg ctgagattag ataaattatt actacaattt gaagaattgt
    42781 atcataatag ctaagataat aaaaccagat taaattatta tatataacgt tgtatgtatc
    42841 agcaaagcta gaaaccaaca cagcagagtt tgcaattgga taaagtataa atataaagat
    42901 agcagctaaa actccatgcg caaatttact tagtatgtat aattttaaat ctatgtccgt
    42961 ttttgataga aatgtacaac attgagctag gatggataat ccactaaatc ctatcaaaaa
    43021 actagctaaa gatgctctta aaatttctgg agaacttgtt atactagata ctttattgca
    43081 tcctatagta atttcaaata gaccacttac aaatgcttga cagagttctt tactaacacc
    43141 taaaaatgaa agaggcaaat atataactga tgctattaat gagattacat taaaaagaga
    43201 tagtatttta aatactactg aaaaaacaat tacaaatcca ccaactgtaa gtaatgtatt
    43261 tactccatta aatacagcat ttccaaataa aacaaaaaaa ccattcccac ttgaatttct
    43321 aatgttgatt acatttttta tatttgtttt taaatccaat tttgtctttg gaagattttc
    43381 attaccataa tttctaaaga aaagaccaac caatatagtt cctagataat ggcagagaat
    43441 cattaaataa cctaagtttg aattttggaa catacctgta ccaaccgaac ctattatgaa
    43501 taaaggtcct gatgtagaac aaaaagacac aagtctttgt gcttcatact tagaaatttg
    43561 tccattactc ctaaattcag aagctaaact tactcctact ggataaccag atactgtaga
    43621 tatagcaaaa gctaaagcgc tctttcctga aacattaaat acaaatctag ttatgggatt
    43681 gatgataaac cctataatat ccactatttt taaatcaaca attaagtttg ctccaattat
    43741 aaatggtagt agtgatggaa ctaaaacatt agaccatata gaaaagcctt ctcttgcaga
    43801 ttttatagct tcatttgggt ataggataat ccctaaaact agaactccaa ctatgataga
    43861 tggagcaaaa aaagcaataa ttttattttt tttcataata tcacctgtaa atttgagtta
    43921 taagatatta tattaaaagt aataaaaata tatgaatgta atggcttaag atatataata
    43981 gatatatatt acaacaacga ttatcatatt ggaggaaaaa atgtttaata gtatagctag
    44041 taaaaaagtt tatgaacaag taattgagca aatacaatat aaaatactaa atggagagtt
    44101 aaaaaagggg gataaattac tatcagaaag agaactgtct gaacaaatga atgtgagtag
    44161 aacttccata agggaagcta ttagggtatt agagactatg ggagtgatag aaagcagaca
    44221 gggtgaagga aattttatat gtacaaatat agaaaaaact ttaatagaac ctttatctat
    44281 gatttttaaa cttaataatg gtacacttga agatatatta gagcttagaa taatcctaga
    44341 aattgagatt gcaaaacttg cttcaaagag aataacttct tctgaagtta tagaattgaa
    44401 acatataata gatgaaatga gggtagaaac taataaaaaa gataataatc gagtactggt
    44461 gttattagac caaaaatttc acagtaaact agcaacatta agtaaaaatt atttaattca
    44521 aagtcttttt atgacagcat caaaattatt tgatggattt atagaagatg caagagaaaa
    44581 aataatcgca gaacctttta atgaaaatat attgcttaaa cagcatgaag ctatatacaa
    44641 tgcagttgta gaaaatgatg tagaacttgc atgtgagaaa gcaaaggaac atatggattt
    44701 tataagtaaa aattatagaa aaaatgaaaa ttaaaagttt gttataaaat aaattattct
    44761 tgcaaatggt agataatggt actatcatat aaatgtaaaa ctggtatgac cacataccaa
    44821 ttttatagtt tattataatg ggagattaaa aatggtaaag ttaaaagtac cgtattcaaa
    44881 acaaggaatg attttagaaa tacctgatga acgttttctc ggtgttttgg agtcaaaatc
    44941 ggataattat atagaacata agactcaaga agagatagtt gaagaatcta tgaataatcc
    45001 tataggctct aattctctgg aagaactggt taaggggaaa aagaatgtag tcataattac
    45061 aagtgaccat acaagacctg taccaagtag aataactatg ccaattatat taagaaggat
    45121 taggaatgtt aatccagaaa ttgatgtgaa gattatagtg gcaactggat ttcataggcc
    45181 aagtacaagg gaagagctaa tttataaaat gggagaagaa atagtagata atgaagatat
    45241 agttatgcat atatctactg atgataaatc tatgtgtaaa gtagggatat tgccttcagg
    45301 tggagattta tatataaaca aactagcata tgaggcagaa cttttgatag cagaaggttt
    45361 tatagaacct catttctttg caggtttttc aggtggcaga aaaagtgtac ttccaggaat
    45421 cgcatctgca aagactataa tgtacaatca ttgtagtgac tttatagata gtgataattc
    45481 aagaactgga aaattgaata ataatcctat tcatgaggat atggtatatg cagctaaagt
    45541 tgcaaaatta gcattcatat tgaatgttgt aatagataaa gataaaaaaa taattgcttc
    45601 ctttgctggt gatgtcgaaa aagcacatac taaaggatgt agatttgtaa cagagttatc
    45661 aaaagtaaat tctataaaat ctgatattgt tgtaactaca aatggtggat atccactaga
    45721 ccaaaatatt tatcaatctg ttaaaggaat gacagcagct gaagctactt gtaaagacgg
    45781 tggagtaata ataatgattt cagcatgtaa tgatggtcat ggaggtcaat ctttttatga
    45841 aaatgtagca aatgcagatt ctccaaaaca acttttagat aaaattagaa gtgtcgatag
    45901 agtagatacg attccagacc aatgggaatt tcagatacta gcgagaatat tatctaaata
    45961 tactgtgata atggtaacag ataagtgtaa tcctgaaatg ataaaaaaaa tgcatatgaa
    46021 acatgcattt aactttaaag aagctttaga aatggcgaat agtatagttg gtaaggagag
    46081 caaagtagta gttatacctg atggagtatc agttatagtt aaataaaaaa ggagtgtact
    46141 ttatggaaat tttgacatgt attaaacaag ttcctggaac gacttcagta gaagttgatg
    46201 aaactacagg agtactaaaa agagatggag tagattcaaa aatgaatccc tatgacctat
    46261 atgctttaga aacagctctt agaataaagg aagataagaa agcgaatcta aaagtactta
    46321 gcatgggtcc tcctcaggca aagaaggtaa ttgaagaaag ttttatgatg ggagctgatg
    46381 aaggagcatt aataagtgat aggagatttg gaggagcaga tgtattggct acttcctata
    46441 ccatatcaca aggtattaag aagatgggaa aggtagacct aataatttgt ggaaaacaaa
    46501 ctactgatgg tgatacagca caggtgggac ctgaagtagc tgaattttta gatatacctc
    46561 atgttactaa cgttacaaaa ttaattgaag ttaaagatga aagtatagtt gttgaaatag
    46621 atatgccaaa tgatttacaa gtatgtgaga ttgaatatcc atgtttaata actgtagaaa
    46681 aagacatttt tcaaccaaga ctaccttcat ttaaattaaa acttaatact aaagatagag
    46741 aaatacctgt atatagctta gatgactttg aagataaaga tgaaaataac tatggactta
    46801 atggttctcc tactcaagtt gtaagaatat ttcctccaaa gccaaataca gataaaaata
    46861 ttgtaagagg aaatgcagat gaactaagtt ttgcacttgt aaataaatta gaggaattaa
    46921 agttggtgta agggggagta ataaatgtca aagatagttg taaatcaaga taaaataact
    46981 gacttaaaga gaattttaga aatatgtcct tttggggcta tagaagaaaa gtcaggtata
    47041 gtagaaataa gtgctggctg taagatgtgc aagttgtgtg tcaaaagtgg accaaaaggc
    47101 gcatttgaat ttatagagag tagtaaagta caaattaata aagatgaatg gaggggaatt
    47161 gcagtatatg ttgaacatca taatggaaat atacatcctg taacgtacga gcttataggt
    47221 aaagctcgtg agatggcatc aaaaataaaa caacctgtgt attgtgtatt tgtaggaaaa
    47281 gacataaaag ataagtgcag taatttgatt tcttatgggg tggatgaagt atttgtatat
    47341 gatgaagatg aatttaaaga ttttaggata gaaccatatt caaaagcaat agaaaacttt
    47401 atagataaaa taaaaccaac gatagtatta gttggtggaa ctacattagg taggtcatta
    47461 gctccaagac ttgccgctag atttagaaca ggtcttacag cagattgtac aatacttgat
    47521 attcaatcaa atactgattt agaccaaata agaccagcat ttggtggaaa tataatggct
    47581 catataaaca cacctaataa tagaccacaa tttgccactg taagatataa aatattttca
    47641 gcaccagaaa aaatagagaa tactacagga aagataactt tatgtaaagt agaaaaaaaa
    47701 gaactaaaat ctaaaattaa ggtactgagt gtaaaagaaa aaaataaaga agttggtctg
    47761 gaagaagctg aagtgatagt tgtggcaagt agagcaataa aaaaacaaga agatatggag
    47821 atgatatata aattagctga taagttaaac gctcaagtgg caggaacaag acctgtaata
    47881 gaagcaggtt ggatagatgc taaaaaacaa ataggtctta gtggtagaac tgtaaaacct
    47941 aaattgataa taacttgtgg tgtatctgga gcagttcaat ttgttgcagg gatgcagggt
    48001 gctgactata tagtagcaat aaataaagat gataaagcgc ctatacttga tgtagcacat
    48061 ttagctttaa taggtgatat ttatgatatt attcctaaat tgattgaaaa aatagaaaat
    48121 aacaaagata ataatcaaaa atatatggca tcagcagcta aataggggga tagaaatgta
    48181 taaattaatc gataaaaaag atatagattt tttaatagat acttgtggag aagaaaatgt
    48241 attagttgga agtgatataa atgaagattt ttcacatgat gaacttggag ggattgagaa
    48301 gtaccctgaa gttttagtaa atgtacttga aactgaacaa gtatctaaaa taatgaaata
    48361 cgcatataaa aataatatac cagtcacacc aagaggtcaa ggtactggtc ttgtaggtgc
    48421 tgctgttgcc ataaatggtg gaataatgat taatctatgt aaaatgaata aaatacttga
    48481 agttgattat gaaaatttaa cattgacagt agaaccaggt gttttattga tgacaatagg
    48541 tcaatatgtt caagatagag atttatttta cccacctgac ccaggtgaaa aaagtgctac
    48601 aattgcaggt aatataaaca ctaatgcagg tggtatgaga gcagtaaaat atggcgttac
    48661 aagggattat gtaagaggac ttgaagttgt actaccaaat ggagaaatta taaatgtagg
    48721 tggaaaggtg gttaaaaata gttctggata tagtataaag gatttattag tgggctcaga
    48781 aggtacttta ggaatagtta ctaaagctat attaaaattg cttcctttac caaagaaaag
    48841 tataagtttg cttataccat ttccagattt atcaatggca atagaaactg ttcctaaaat
    48901 aataaaatca aaatcaatac caacggctat agaatttatg gaaagagatg tgatattggc
    48961 agcagaagaa tttttaggta aaaagtttcc agataataca tcagatgctt atcttttgct
    49021 tacatttgat ggaaatagca ctgaagatat agaaaaagaa tatgaaaaag tagctaattt
    49081 atgtttagaa aatggtgcat tagatgtatt tatatcagat acacaagaaa gaaacgattc
    49141 tatatggtca gccagaggtg catttttaga agctataaag gcatctacga cacaaatgga
    49201 tgaatgtgat gtcgttgttc caagagataa gatagcagag tttataagat acactcacga
    49261 gcttcaagac aaattaaaaa taagaataaa aagttttggt catgcaggag atggaaatct
    49321 acacatatat attttaaaag atggtatgga tgataataca tggaaaataa gattaaaaga
    49381 aacttttgac tatatgtata aaaaatctag agaacttagt ggtcaagttt ctggtgaaca
    49441 tggcatagga tatgctaaaa aagagtactt acatgaatca aattcagatg cttatatgat
    49501 gttaattaaa aatataaagt tagcttttga ccctaaaaat attttaaatc cagggaaaat
    49561 atattagaaa tattaattta ataatattta taaaacaaaa aatattaaat tagatttaaa
    49621 tataatattt taaactttac ttttaaatct gaagctaaat tgtaaaattt ttaggatggc
    49681 aatatttact taaatttaga aagtaacata atatttaagt tgatgttgtc atcctaaaag
    49741 catgtgtaca taaataaagt aagactattg tatcttatat aataaagagt taataaaatg
    49801 atttttataa atatagttat tttaaaaaac tactttacat atattggatg agtataaaaa
    49861 tccatagggc cttttactaa atctttattg tttttataat atattgaatt atatatattt
    49921 gtagctttta tatcataact tatgagtgtt ttaaaaatcg tatcatctaa tgaaaatgat
    49981 atatttgaaa acttgtttat tattttt
//
LOCUS       DAGSHT010000009        49075 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGSHT010000009 DAGSHT010000000
VERSION     DAGSHT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.010"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(121..894)
                     /locus_tag="MJB72_000471"
     CDS             complement(121..894)
                     /locus_tag="MJB72_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBY8261647.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="MJB72_000472"
     CDS             complement(894..1280)
                     /locus_tag="MJB72_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8261648.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="MJB72_000473"
     CDS             complement(1305..1622)
                     /locus_tag="MJB72_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBY8261649.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="MJB72_000474"
     CDS             complement(1634..1894)
                     /locus_tag="MJB72_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBY8261650.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="MJB72_000475"
     CDS             complement(1894..2190)
                     /locus_tag="MJB72_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY8261651.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="MJB72_000476"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="MJB72_000476"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBY8261652.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="MJB72_000477"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="MJB72_000477"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBY8261653.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="MJB72_000478"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="MJB72_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBY8261654.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="MJB72_000479"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="MJB72_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBY8261655.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="MJB72_000480"
     CDS             complement(8083..9189)
                     /locus_tag="MJB72_000480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBY8261656.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="MJB72_000481"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="MJB72_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBY8261657.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="MJB72_000482"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="MJB72_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBY8261658.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="MJB72_000483"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="MJB72_000483"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBY8261659.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="MJB72_000484"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="MJB72_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBY8261660.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="MJB72_000485"
     CDS             12283..12990
                     /locus_tag="MJB72_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBY8261661.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="MJB72_000486"
     CDS             12959..13588
                     /locus_tag="MJB72_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBY8261662.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="MJB72_000487"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="MJB72_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBY8261663.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="MJB72_000488"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="MJB72_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBY8261664.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="MJB72_000489"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="MJB72_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBY8261665.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="MJB72_000490"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="MJB72_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBY8261666.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="MJB72_000491"
     CDS             18864..19637
                     /locus_tag="MJB72_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBY8261667.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="MJB72_000492"
     CDS             19639..20502
                     /locus_tag="MJB72_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBY8261668.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="MJB72_000493"
     CDS             20535..21677
                     /locus_tag="MJB72_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY8261669.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="MJB72_000494"
     CDS             complement(21810..22385)
                     /locus_tag="MJB72_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBY8261670.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="MJB72_000495"
     CDS             22645..23937
                     /locus_tag="MJB72_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY8261671.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="MJB72_000496"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="MJB72_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBY8261672.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="MJB72_000497"
     CDS             24717..24980
                     /locus_tag="MJB72_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBY8261673.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="MJB72_000498"
     CDS             24995..25180
                     /locus_tag="MJB72_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBY8261674.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="MJB72_000499"
     CDS             25283..26479
                     /locus_tag="MJB72_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY8261675.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="MJB72_000500"
     CDS             26693..26971
                     /locus_tag="MJB72_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBY8261676.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="MJB72_000501"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="MJB72_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY8261677.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="MJB72_000502"
     CDS             27461..27688
                     /locus_tag="MJB72_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBY8261678.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="MJB72_000503"
     CDS             27831..28151
                     /locus_tag="MJB72_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBY8261679.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="MJB72_000504"
     CDS             28159..29115
                     /locus_tag="MJB72_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBY8261680.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="MJB72_000505"
     CDS             29126..31120
                     /locus_tag="MJB72_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBY8261681.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="MJB72_000506"
     CDS             31121..31570
                     /locus_tag="MJB72_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBY8261682.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="MJB72_000507"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="MJB72_000507"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBY8261683.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="MJB72_000508"
     CDS             33122..33430
                     /locus_tag="MJB72_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261684.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="MJB72_000509"
     CDS             33512..34801
                     /locus_tag="MJB72_000509"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBY8261685.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="MJB72_000510"
     tRNA            35127..35202
                     /locus_tag="MJB72_000510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="MJB72_000511"
     tRNA            35209..35283
                     /locus_tag="MJB72_000511"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="MJB72_000512"
     tRNA            35288..35363
                     /locus_tag="MJB72_000512"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="MJB72_000513"
     tRNA            35397..35471
                     /locus_tag="MJB72_000513"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="MJB72_000514"
     CDS             complement(35854..36846)
                     /locus_tag="MJB72_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8261686.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="MJB72_000515"
     CDS             complement(36855..37913)
                     /locus_tag="MJB72_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBY8261687.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="MJB72_000516"
     CDS             38038..39501
                     /locus_tag="MJB72_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBY8261688.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNLDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFNVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="MJB72_000517"
     CDS             39603..40400
                     /locus_tag="MJB72_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8261689.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="MJB72_000518"
     CDS             40413..42515
                     /locus_tag="MJB72_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBY8261690.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="MJB72_000519"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="MJB72_000519"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBY8261691.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43129..44820
                     /locus_tag="MJB72_000520"
     CDS             43129..44820
                     /locus_tag="MJB72_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261692.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKYSIKNVVSISSILMQNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQI
                     KFPLIYIDEDSFCPYCLGNKFNIINNIKQQDIKVECEKCGEAISYFIHKEEVDNIIEE
                     EKRKKELFIRTLENVYNKINKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEI
                     AQEQYRLWQQRAAMMANIKNKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDL
                     VDMQDRGENIFKQLFNNYVRLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGI
                     TYSEAVVYELMEKTKLLPIRAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESIN
                     KSQNINNQVAYLIFNKQKYICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYED
                     ITEDEIIIDNIFCVVDERNNELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNL
                     IRAYANTLYKQTKDGFANEKVFFAYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
     gene            45052..46524
                     /locus_tag="MJB72_000521"
     CDS             45052..46524
                     /locus_tag="MJB72_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261693.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVKQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEIFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDTVYGIPQHLLLEVIKNGKEFFTK"
     gene            46499..47563
                     /locus_tag="MJB72_000522"
     CDS             46499..47563
                     /locus_tag="MJB72_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261694.1"
                     /translation="MEKNFLLNKINSIQKEYLELLVKLKDNIDTIDYIYIIDEINIFW
                     YSYRDVIKLYLGNITQKDNAYIFTGATYLDVDDLEHYPFVMMGDIHIVDDSLCKYSKT
                     VLVLSNQEFAKVLKDQIISCIDDNIKILSNYSFAMLILPMNLLYGNDMKIIHEYSNNI
                     FLNFFSRKDLNLDTYFEEFNSIEDIALALNPGIESYIVFDEGEDLTKNLEERFKSFID
                     KGKVPLPAHKSDAFKFWLIIYGFITQALDIILSCKGFNLTPYLRYRVTLQYVLLLGDN
                     VFDIEDLSNMLLKCKVAHVLYAIFDKSEIKNISFVQFYKKLSEYKFSYKVENDIKENI
                     NINQIAEILNKNITLFLNYI"
     gene            47703..48875
                     /locus_tag="MJB72_000523"
     CDS             47703..48875
                     /locus_tag="MJB72_000523"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8261695.1"
                     /translation="MLKQNTIQIDYTTYGNNYQFKLPLNIDYMIPKDAIFVDGTKIES
                     SANRYTFVWKKTINKNMVRVMNDISNFILKCGIDFGIKIIYRNKIKIYHLKKLLKKLK
                     KISKESNITFVHGKGKKKSIVQKSIAKLNNYIDRLKKYTKDLHIMGELNSYSKTDNDA
                     TFMRMKKDHMKNGQLKPAYNIQFGVDSEYIVWVSSEHKPTDTTTSIPFLKSIEEHTKF
                     KYPKIVADSGYESEENYTFLECNNQLSFIKPANYEISKTRKYKNDIGRIDNMDYDEKK
                     DYYICKNGKKLFANKIINRKSKTGYKSKITCYTCKECCDCQYKNGCIKGNNSKIPLEN
                     RTKNLQVSKLFHEKRKENLKRIISQEGCQLRMNRIIQAEGTFAQVKQNMKFRRFLS"
BASE COUNT        18724 a         6005 c         7805 g        16541 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtgcatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tgggttttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca acgagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttccta
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataaaat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ctctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagctcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttca
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggtcc ctaagaacct
    21781 ctaatctatt tgataattgt tttaagaagt tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagaataa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa acttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtattcat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttacg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattgaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaaccg ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagagtct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaaca taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagaacaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt ataggaccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttta cttacattga ttttttatta agttaaagct
    34921 acttatacat attctagctg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 atagtattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taagatacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag actctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aagtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcgcttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacctt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tctccattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag agacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacttaga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagcgga aaacgttaga gattttgcat ttatactaag tagcaaattt
    38821 aatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagcga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcctt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aacagtgaat gtaagtaaat tttgtgtaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aagaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttga
    40261 gtaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aacggctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtgataatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac catcacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt caagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gaacttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctctagggct ttctgatgag gttttaggaa gggctgatta taggttatct
    42961 ttttctagga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gttttttatg
    43081 atataattga attataaaat aaaatcaaaa tgtaaagtag gtgtagtaat ggacagacta
    43141 gatatattta tcgaaaatta tgattttaaa gaatatcttg aaaaattacc tagagaatta
    43201 atagaagaaa attataaaga ttttgttata gaattttgga aaaatcctag tgtagatatt
    43261 attaaactaa aatcaaaata tagtataaaa aatgtagtat ctataagtag tatattaatg
    43321 caaaatccta tccaagagta ttatgagttt cttgaagata tttggaaaaa taacataaaa
    43381 atagatgaat taattaataa gtatttaatt acagcaacaa gaatggatca aattaaattc
    43441 ccattaatat atattgatga agattctttt tgtccttatt gtttaggtaa taaattcaat
    43501 attattaata atattaagca acaagatata aaagttgaat gtgaaaagtg tggtgaggca
    43561 attagttatt ttattcataa ggaagaagta gataatataa tagaagaaga aaaaagaaaa
    43621 aaagaactat ttataagaac attggagaat gtatataata aaataaacaa gattaaatgt
    43681 catagatgcc aatcagattt atatgtgtgt attaatgata aaataatgga gtataatatt
    43741 aggtgtgata gatgtaattc acagtatgaa tcggttgaaa ttgcacagga acaatataga
    43801 ttatggcaac aaagagctgc aatgatggct aacataaaaa ataaagaaaa tgaattgttg
    43861 ctaaagacac tatcaattaa ggacgaaaaa gatattaaat ttaaactcga agatataatt
    43921 acggagagtg aaagtataga tgtaattagt gatttagtag atatgcaaga tagaggagag
    43981 aatattttca agcaattatt taataattat gttagactag gaagattaga cagaaggtta
    44041 ttggtaactg tgtgtaaaga ttgcaagcaa aatgaaaata cttatacatg ggaaagaggc
    44101 aaagaacatt taataaatat gagggggatt acatacagtg aagcagttgt atatgagtta
    44161 atggaaaaaa caaagctatt gccaataaga gcaattatta gaagacttat ggagaaaaaa
    44221 ctattgtatt gtgatgaaag gagcaataat ttacaagtac accctgtttt gattgaaaat
    44281 ttagctaaac tagaatcaat aaataaatct caaaatataa ataatcaagt agcttatcta
    44341 atattcaata agcaaaaata tatatgttat cattgtggtg aatcaggaag acccttaaag
    44401 ataggatatc taacaataga taaaaatata aatgatttat caagtttagt gggaatatgt
    44461 gatagttgct atgaagatat tactgaagat gaaattatta tagataatat tttttgtgtg
    44521 gtagatgaaa gaaacaatga attaggggaa gcttggaaat ttttaataca atatgatagt
    44581 agttttagaa ataatacaat tgcagaagta gagcatgcaa gattatatga tatgtatgat
    44641 gaagataatt taattcgtgc gtatgcaaat acattgtata aacaaacgaa agatgggttt
    44701 gctaatgaaa aagtattttt tgcatatgca agagcaatat tgaaaaatag caaagggcaa
    44761 gtaagtgtac tacaaatagc aaaagaaaaa tttaaattag aaaagtggtt aaatggataa
    44821 agggataata catattaaaa ttataaattt atataaaact gtattgcaat taagcaatac
    44881 ggtttttata tataagataa tgatacaatt aaccaaaatg tggtagtaca taagtgttat
    44941 gagatttaaa tattagcgat taaaataatg attaagtaaa attgtaattg atgaggagta
    45001 ttaacgtatt agtttatttt tatatataat taaaatatag gagggaagaa tgtgagtcat
    45061 agtaaactat ctgatcacaa attaaaaaag gggaaattta tagcaccttt taatgaattt
    45121 atgacaccat tgaatagtaa tgagtcatgg tgccttggtc gattgccaga gtatatatgg
    45181 ataggattat taataaataa atatggtaga aaaaaaggga tggaaataat ttataaaata
    45241 attgtaaaac ttcattcaat tgaatcagat attgtaacac ctaggttgtc tgagatttta
    45301 tctatggagg atgtaaagca ggatgaattc tttagttata ttctaactat tgttgaaaag
    45361 gaagctttat ctccattgac attaatattt acatatagta attatcctat ttttgttaaa
    45421 tattttgcag agccaagtat aaaaattgaa gatagggtta cctatttaga tgagacttta
    45481 agaaaagcta tgcatcatca aagtgaattt gctacagaca taagatttat agttttatat
    45541 tatttttgta tgtcaggaaa actcgtattg caaaaagaac agattaataa tttgctggaa
    45601 tatcctaatt tagaacacac tgatgaaaaa atgagaatgt tacgtccgtt tattcgtgca
    45661 gcagaattaa tggttttaaa tatggaaaaa aaatctgatt ggtatttaga tattttttgg
    45721 gggaggatta gtgttatgac tgaatgtaaa ttatacgcaa ctaaatttga agatgaaact
    45781 gctaatacag acttgtatat ggaacaatta catgagattt ttgtttattt atcagattta
    45841 ctatgtaaag taaatccatt agacgagaaa atgttagtat tacttggaat agcaatatat
    45901 tcttataaac gattacgaga agtagttgag cataaattat ttaattccat ttcaggtaga
    45961 ggctcagtaa gggtattgat agaaaattat attatgatga agtatttagt aaaaatagaa
    46021 gctcaacatg aaaatatatg gtcagaatat caattttatg gaataggtca atataaatta
    46081 attgtggcta gatatagaga acaagaacaa gatttaaaaa atagtcatgt tgattataaa
    46141 tatattgaag ctttagttaa tgaatttgta attgaagaaa caatagatat ggatactaaa
    46201 tattttgata agcaaaatat acgtagtaag gctgaaatag ttggagaaaa agagttatat
    46261 gggttgtact atgattatga ttccgcatat gaacatggtt tatggggatc aataagagaa
    46321 agttcaatgt taaagtgtga taatccagct catcactatc attgtatacc agatgtagac
    46381 aataatcaaa atttaaaaag tgtttggcat gattgtgtaa acataatgga aaaaacttta
    46441 cttttcttag atactgtata tggtatacca caacatttgc tattagaggt gataaaaaat
    46501 ggaaaagaat tttttactaa ataaaataaa tagtattcag aaggaatatt tagaattatt
    46561 agtaaagtta aaagataata tagatactat agattatatt tatataatag atgaaataaa
    46621 tatattttgg tattcatata gagatgttat aaagctttac ttagggaata ttacacaaaa
    46681 agacaatgca tatattttta caggtgcaac atatctagat gttgatgatt tagaacatta
    46741 tccatttgtt atgatgggag atatacatat agtagatgat tcgctatgta aatattcaaa
    46801 aacagtacta gtattatcaa atcaggaatt tgctaaagta ttaaaagacc aaattatttc
    46861 ttgtatagat gataatatca aaatattgtc taattattct tttgcgatgt taatattacc
    46921 aatgaattta ctatatggta atgacatgaa aataatacat gaatattcaa ataatatatt
    46981 tctgaacttt ttttcaagaa aagacttaaa cttagatact tactttgaag aatttaactc
    47041 aatcgaagat atagctctag cattgaatcc aggtatagag agttatattg tttttgacga
    47101 aggtgaagat ttaactaaaa atctggaaga aagatttaaa agctttattg ataaggggaa
    47161 ggtaccccta ccagcacata aaagtgatgc ttttaaattt tggctgataa tttatggatt
    47221 tataactcag gcattagata ttatattgtc atgtaaagga tttaacctaa ctccttattt
    47281 aagatataga gttacattac aatatgtttt attactaggc gataatgttt ttgatattga
    47341 agatctcagc aatatgttgt taaaatgtaa agtagctcat gtactttatg caatatttga
    47401 taaaagcgaa ataaaaaata tttcctttgt tcaattttat aagaaattaa gtgaatataa
    47461 atttagctat aaagtagaaa atgatatcaa agaaaatata aatataaatc aaattgctga
    47521 aattttaaat aaaaatatta cattgttttt aaattatata taaatttatt tatataatta
    47581 cattccttag ggtacataac agaaaaaagg ctgtcgcact aaaaaatagt gctacgccct
    47641 tttttataca aaaaaataaa gctcaagttc aaaagaacct gggattcctg tataataatt
    47701 gtatgctaaa acaaaatact atacaaattg attatactac ttatggtaat aattatcaat
    47761 ttaaattacc actaaatatt gattatatga ttccaaaaga tgctatattt gttgatggta
    47821 caaaaataga gtcatctgcc aatagatata cttttgtttg gaaaaaaact ataaacaaaa
    47881 atatggttag agtgatgaat gatatatcta atttcatatt aaaatgtggg attgattttg
    47941 gtattaaaat aatttataga aataaaatca aaatatatca tttaaaaaaa cttttaaaga
    48001 agcttaaaaa aatatcaaaa gaatcaaata taacatttgt tcatggaaaa ggaaagaaaa
    48061 aatctattgt tcaaaaatct atagcgaaat taaataatta tatcgataga ttgaaaaaat
    48121 atactaaaga ccttcatata atgggtgaac taaatagtta ttctaaaaca gataatgacg
    48181 caactttcat gagaatgaag aaagaccata tgaaaaatgg gcaattgaaa ccagcatata
    48241 atatacagtt tggcgtagat tcagaataca tagtttgggt aagttcagag cataaaccta
    48301 ctgatactac aacatcaata ccatttttaa aatctattga agagcataca aaatttaaat
    48361 atcctaaaat agtagctgat tctgggtatg aaagtgagga aaactataca tttttagagt
    48421 gtaataatca attatctttt ataaagcctg caaattatga aattagtaaa actagaaaat
    48481 ataaaaatga tattgggcgt attgataata tggattatga tgaaaaaaaa gattattata
    48541 tttgtaaaaa tggcaaaaaa ctatttgcta ataaaataat caatagaaaa tcaaaaacag
    48601 gatataaaag taaaataact tgttatactt gtaaagaatg ctgtgattgt caatataaaa
    48661 acggttgtat taagggaaat aatagtaaga ttcctcttga aaatagaact aaaaatttac
    48721 aagtatctaa gttatttcat gaaaaaagaa aagaaaattt aaagagaatt ataagccaag
    48781 aaggttgtca acttagaatg aatagaatta tacaagcaga aggaactttc gctcaagtaa
    48841 aacaaaatat gaaatttaga agatttttaa gctgaggcaa agaaaatgtt atatcagaat
    48901 gtattattgt agcgatagct cataacattt ctagactaca caacaaaata ataaaaaata
    48961 aatgtggaat atatcttcat catttaaaag aagtattcta aatttattat ttatagttag
    49021 atattatctg aaaaaatggt atttttatac ctttatttgt tatgcataat tttac
//
LOCUS       DAGSHT010000010        45795 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGSHT010000010 DAGSHT010000000
VERSION     DAGSHT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.011"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            410..1627
                     /locus_tag="MJB72_000524"
     CDS             410..1627
                     /locus_tag="MJB72_000524"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBY8261696.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1722..2102
                     /locus_tag="MJB72_000525"
     CDS             1722..2102
                     /locus_tag="MJB72_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY8261697.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2386..2868
                     /locus_tag="MJB72_000526"
     CDS             2386..2868
                     /locus_tag="MJB72_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8261698.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2889..3704
                     /locus_tag="MJB72_000527"
     CDS             2889..3704
                     /locus_tag="MJB72_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8261699.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3755..5305
                     /locus_tag="MJB72_000528"
     CDS             3755..5305
                     /locus_tag="MJB72_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY8261700.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5402..6754
                     /locus_tag="MJB72_000529"
     CDS             5402..6754
                     /locus_tag="MJB72_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY8261701.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6885..7643
                     /locus_tag="MJB72_000530"
     CDS             6885..7643
                     /locus_tag="MJB72_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261702.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7690..8409
                     /locus_tag="MJB72_000531"
     CDS             7690..8409
                     /locus_tag="MJB72_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY8261703.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8533..9933)
                     /locus_tag="MJB72_000532"
     CDS             complement(8533..9933)
                     /locus_tag="MJB72_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBY8261704.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9937..10845)
                     /locus_tag="MJB72_000533"
     CDS             complement(9937..10845)
                     /locus_tag="MJB72_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8261705.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            11106..11753
                     /locus_tag="MJB72_000534"
     CDS             11106..11753
                     /locus_tag="MJB72_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY8261706.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            12078..14717
                     /locus_tag="MJB72_000535"
     CDS             12078..14717
                     /locus_tag="MJB72_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBY8261707.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14775..15878)
                     /locus_tag="MJB72_000536"
     CDS             complement(14775..15878)
                     /locus_tag="MJB72_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBY8261708.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16239..17060
                     /locus_tag="MJB72_000537"
     CDS             16239..17060
                     /locus_tag="MJB72_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY8261709.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    17139..17350
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17437..18753
                     /gene="argH"
                     /locus_tag="MJB72_000538"
     CDS             17437..18753
                     /gene="argH"
                     /locus_tag="MJB72_000538"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBY8261710.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            19002..19340
                     /locus_tag="MJB72_000539"
     CDS             19002..19340
                     /locus_tag="MJB72_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBY8261711.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19467..20756)
                     /locus_tag="MJB72_000540"
     CDS             complement(19467..20756)
                     /locus_tag="MJB72_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY8261712.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20836..21540
                     /locus_tag="MJB72_000541"
     CDS             20836..21540
                     /locus_tag="MJB72_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBY8261713.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21632..22678
                     /gene="selD"
                     /locus_tag="MJB72_000542"
     CDS             21632..22678
                     /gene="selD"
                     /locus_tag="MJB72_000542"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21680..21682,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBY8261714.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22696..24108
                     /locus_tag="MJB72_000543"
     CDS             22696..24108
                     /locus_tag="MJB72_000543"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBY8261715.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24222..26105
                     /gene="selB"
                     /locus_tag="MJB72_000544"
     CDS             24222..26105
                     /gene="selB"
                     /locus_tag="MJB72_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBY8261716.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26210..28948
                     /locus_tag="MJB72_000545"
     CDS             26210..28948
                     /locus_tag="MJB72_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBY8261717.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(29067..30011)
                     /gene="manA"
                     /locus_tag="MJB72_000546"
     CDS             complement(29067..30011)
                     /gene="manA"
                     /locus_tag="MJB72_000546"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBY8261718.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            30184..31545
                     /locus_tag="MJB72_000547"
     CDS             30184..31545
                     /locus_tag="MJB72_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8261719.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31633..33522
                     /locus_tag="MJB72_000548"
     CDS             31633..33522
                     /locus_tag="MJB72_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8261720.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDIAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33537..33992
                     /locus_tag="MJB72_000549"
     CDS             33537..33992
                     /locus_tag="MJB72_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8261721.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            34051..34368
                     /locus_tag="MJB72_000550"
     CDS             34051..34368
                     /locus_tag="MJB72_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY8261722.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34393..35655
                     /locus_tag="MJB72_000551"
     CDS             34393..35655
                     /locus_tag="MJB72_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY8261723.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35760..36824
                     /locus_tag="MJB72_000552"
     CDS             35760..36824
                     /locus_tag="MJB72_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY8261724.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36878..38056
                     /locus_tag="MJB72_000553"
     CDS             36878..38056
                     /locus_tag="MJB72_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBY8261725.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38247..39305)
                     /locus_tag="MJB72_000554"
     CDS             complement(38247..39305)
                     /locus_tag="MJB72_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBY8261726.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39372..39743
                     /locus_tag="MJB72_000555"
     CDS             39372..39743
                     /locus_tag="MJB72_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBY8261727.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39780..40454
                     /locus_tag="MJB72_000556"
     CDS             39780..40454
                     /locus_tag="MJB72_000556"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBY8261728.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40703..41542
                     /locus_tag="MJB72_000557"
     CDS             40703..41542
                     /locus_tag="MJB72_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBY8261729.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41786..41882
                     /locus_tag="MJB72_000558"
     tRNA            41786..41882
                     /locus_tag="MJB72_000558"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41820..41822,aa:Sec,seq:tca)
     gene            42033..42737
                     /locus_tag="MJB72_000559"
     CDS             42033..42737
                     /locus_tag="MJB72_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBY8261730.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLG"
     gene            complement(42882..43718)
                     /locus_tag="MJB72_000560"
     CDS             complement(42882..43718)
                     /locus_tag="MJB72_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8261731.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43906..44811
                     /locus_tag="MJB72_000561"
     CDS             43906..44811
                     /locus_tag="MJB72_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBY8261732.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44820..>45795
                     /locus_tag="MJB72_000562"
     CDS             44820..>45795
                     /locus_tag="MJB72_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8261733.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSV"
BASE COUNT        17951 a         4977 c         7910 g        14957 t
ORIGIN      
        1 tgtatagttt tctaattttg aataaaaata aagtaaaact ttcttttata ttgaagttgt
       61 ttagtaagac atgtatttta ttaatttatt aatcttaata atgttaaata aaatcaaaaa
      121 aatagatttt gggtaaataa aataatataa aatataaatt ttaataaaaa taataaagta
      181 tatattattg ctttaataag aatgtaaaaa atatttttaa attatcattt aatgtaattt
      241 aaatgaattg aagatatatg tttattgtat gcaattgttg tatttttagg ttttttgtga
      301 ttaattaaaa aattataaag atcgtttaca tgttgatttt taaaaaaaaa agatatagaa
      361 ttatatagaa agtaataaac aaaaattgga tttacgggag tggaatgaaa tgatgaatgt
      421 taccttaaca gatgtgaaag aggctagaga gaccattaag aacatagtaa aaagaactga
      481 cttacttgaa agtgttaaac ttagcgaaaa aacaggagca aatgtgttct ataaatgtga
      541 gaatcttcaa aaaacaggtt cttttaaatt aagaggagct tgtaacaaaa tagcgagttt
      601 aacagatgaa gaaaaggcaa atggagttat agcttcaagt gcagggaatc atgctcaagg
      661 agttgcattg ggagcaaaaa tgactggtat aaaatctaca atagtaatgc cagcaactgc
      721 gcctttagca aaagttagtg caactaaagg ttatggagca gaagtagttt taaatggtgc
      781 ggtttatgat gatgcatatg caaaagctgt tgaaatacaa aaagaaacag gagcaacttt
      841 cttacatcca ttcaatgata agtatgttat agcaggacaa ggaactatat ctcttgaaat
      901 tttcgaacaa ttagacaata aagttgatac tatactatgc ccagttggtg gaggaggaat
      961 catctctggt gttgctgttg cagcaaaagc attaaatcct aatgtaaaaa tagtcggtgt
     1021 tcaaactgca aatatacctt caatgaaaga gtcaataaaa aatggaaaag taacaacagc
     1081 atttaatgat actacaatag ctgatggtat agctgttaaa actccaggag atttaacttt
     1141 tgaaataata aatgaattgg ttgatgaaat agtagtggtt gaagagacag aaattgcaga
     1201 atcaatatta tttatgatgg aaagtcaaaa aatagtttca gaaggtgctg gtgctgtatg
     1261 tacagctgct atattaagtg gtaagtatgt accagcaaaa gatgaaaatg tagtttgtat
     1321 aatatctggt ggtaatatag acataaatac gttatataga ataataggtg ttgctttagc
     1381 taaagaagga agaagatatt catttagtac aattatggaa gataagccag gaaactttgc
     1441 tgagttaact agaataataa gtgagaatgg tggaaacatt ttaagtgcta atcaaggtaa
     1501 attatcagca ggggaagctc taggaaaaca aagtgcggaa tttatattag agacaataga
     1561 ttatgaccat atagctagaa taaagaaagc tatagaagaa aaaggtttta aaattataga
     1621 attataataa aaagtgtata taatatacaa gaaaaatagt ataatagtgt atgaagatac
     1681 attttatgtg tattagatta acgaattagg aggattttaa aatgaaacat gaagtaatac
     1741 atactaatga tgcaccagca gctttaggac catattctca agctataaaa gctggaaact
     1801 tgttatttgt atctggacaa gttcctcttg taccagagac tatggaagta gttgaaggag
     1861 atgtacaagc tcaaactgct caatctttaa aaaatcttaa agctatatta gctgaaagtg
     1921 gagctgattt ttcaaatgta gttaagacta cagtatttat aaaagatatg aatgaatttg
     1981 gggctataaa cgaagtttat gctgaatatt ttggagaaaa taaaccagca agagcttgtg
     2041 tagaagttgc tagattacca aaagacgtta aagttgaaat agaagtaata gctgtattat
     2101 aaaaatacaa tatttttaat ttgaaaaggc tgaaaaacta tattctagtg attcagcctt
     2161 tttataaaaa aataaatcac aataaagaac atgataaata ttgtcgaatt aaaaaataaa
     2221 attaaaataa atattgcaat atttttgaga aggttttata atgagtatat aatttgaaac
     2281 gtgtaactat taaattaggc atgagttctt ggatattttt atcctgagga ttcatgcctt
     2341 ttttgtattg aaattcaaaa atctatttaa taaggaggaa gtactgtgtt aaattttttc
     2401 aagaaaaata aaagctataa gttacatgca gtagtgagtg gtaacagcat taatattgag
     2461 aaagtaaacg attctgtgtt ttcaaaaaag ttaatgggag atggagttgc aattattcct
     2521 aatagtaatg ttgttgtagc cccttgtaat ggtaaagtaa ctgtgcttac agaatcaaag
     2581 catgcctttg gtatggtctc agatgagggt gttgaaatat tagtccacat aggaattgat
     2641 acagttagtt tacagggtga ggggtttaaa aatgaagtgt ctcaaggaga tactgttaaa
     2701 aaaggtagtc caataataag ttttgaaaga gaaaaaataa attctcaggg aattgattgt
     2761 acaacaataa taattgtatt aaatcatagc gaattttcag aaataaattg catggtagaa
     2821 aatgaagttg ttgcagggca agatactgtt attgaaatta tgaaataata ggaagggatt
     2881 aattcaatat gagagtagta aaaatcttta ataataatgc attatctaca gtaactaatg
     2941 ataggaaaga agctatttta ctaggtcttg gtataggttt taataaacgt ccaggagata
     3001 aagttaatga agataaaata gaaaagattt attatgttca agaccacatg caaacaaaat
     3061 ttttagaatt attaaaaaat gttagtccag aagtgatgga tgcttcacaa caaattataa
     3121 gtcttatacc aggaaatgag ggaaaattta ataataaagg gatattatct ttagtggaac
     3181 atattagttt tgcaatagat agaatgaaaa ataatgtttt tttacctaat ttaatgcttt
     3241 tggatataaa gatgatgtat tcaaaagagt ttgaattagg agtaaaagct ttagaaatta
     3301 tatatagagt atgtcatatc catttgcctg aagatgaagc aggttacata gctttacatt
     3361 ttgtcaattt acaatcaaat gataatttgg catatgatac tttaaaattt gtaaaaggta
     3421 gtattgattt gattaaagaa tgttatggat tagaattaga cgaatcgagt ttaagtacat
     3481 tacgttttag aactcatctt aaatttcttg ctcaaagaat ttttaagaat gaaatttgtc
     3541 aggatgataa aatgattgaa atgtatgatt atttaattaa caatcatcct aaaaataaag
     3601 aatatttaga aaagttaaat gtatatattg aaaaggagtt taggtataaa ttagataaac
     3661 ctgaaaaaat ttatctacta ctacacctaa caaaaatctt ataagaatga gatgaattag
     3721 taatagaaat atataatttt agggggggat aagattgaaa gaaaaactac ttggaggact
     3781 ggaaacattt gcaaaggcaa tggttcagcc attaatgtat ttatcgagtg caggaatatt
     3841 aatggtggta ggtgtactac ttacaaatga tatgttatta ggagtacttc cattttttaa
     3901 atgggagcca attcaaatat ttggaagcct aatttataac tgtataatgg ctattattaa
     3961 taatcttgct gttatatttg ctataggtat tcctgctgca ctagcaaaaa aagagaagca
     4021 caaagcagcg ttaataggat ttatgagtta tataatatac cttacagctt ccaacactat
     4081 gttaacatca ctaggtcaac tagcaaaaga aagcaagatg cttggactga tagggactgg
     4141 acaagctact attttaggta ttcaagttgt agatacaagt gtatttggtg gaattatttt
     4201 agggtgtata gctggatatg tatttaatcg tacaagcaac aaaacattta aaggagcttt
     4261 acagatatac tcgggagcaa attttagttt tatatgcatg ttctttgtat ccattttaat
     4321 tggagttgtg agtaacttta tatggccatt agctcaacaa ggaatagctt ctcttgcaaa
     4381 tgtcatcaaa gatacaggta catttggttt attcttatat ggttttttag agagaatatt
     4441 agtaccaact ggacttcatc atttaatcta tacaccattt cagttttctg atttaggagg
     4501 agtgcttaaa gttggtgcag atacagtttc aggagcctat gctatagtta tgacagaaat
     4561 gaatttacca gttgagcgtt tctcagatag tatatactac atggcaactg gatttacaaa
     4621 gatgtttggt tatataggta taggagctgc ctttatttat actgcatata agcaaaataa
     4681 gaagaaagtt aaggcaacga ttattccact aataattacg gcagtattag cctctgtaac
     4741 agaaccatta gactttatgt ttgtattctg tgcaccattt ttattctttg tacatgcagt
     4801 tatagctggt ctttttatag ctttattaaa aatatttgat gttactgcat tatgtggagg
     4861 gaacttatta gtatcaatta taatgaatac tacagtaggc gtagaaaaaa cacactggcc
     4921 agtaatgatg atattaggac ttgcacaaat tattgtatat tttgtagtat ttagtttttt
     4981 aattaagaaa tttaattata aaactccagg tcgtgaagat gagagtacag ctacaggcga
     5041 agaacaatca aaacaactta atttagatgc agggatagaa aatataattg atgggttagg
     5101 cggaaaagaa aatattaata cagtagaaaa ttgcatcact agattacgtg ttaatgtaaa
     5161 agatgaaagt aagattaatg aagatataat taatttaacg ccaaatagcg ggattgtaag
     5221 gaaaggaaaa gatatacaaa taatttttgg acttcatgtg catgaggtaa gacgtgcagt
     5281 agaagatttt ttagaagctt attaaagata tatatttata tagcaagggt aaataacagc
     5341 tttaagtgct gtttactatt tattaaagat acatataatt tatagaggag gaataattat
     5401 tatgattatt actattgttg gtggaggaag cacatttact ccaggtattg ttaagtcaat
     5461 tgcattaaga gaaaaagagt tagagcttga ggaaattcgc ttatttgaca ttgataaaga
     5521 acgccaagat aaagtagctg tagttgtcaa ctggatacta aaagaagatg ttaaaacaga
     5581 tgttaaacta actgtaacta attgtgaaaa agaagcgtat acagatgcta catttatatt
     5641 tgcacaaatg cgtgttggta aatatgaaat gcgtgagcag gatgaaaaaa ttccattgaa
     5701 atatggttgt gtaggtcaag aaacttgtgg ttgtggaggg atggcatacg gactgcgtac
     5761 aatacaacca atgataaagg ttattgatga tgtagagaaa tatgctaaaa aagattattg
     5821 gatattaaat tactctaatc ctgctgcaat cgtttctgaa gcttgtcgta agttaagacc
     5881 aaatgcaagg attataaata tctgtgatat gccaatagct attatagata tgatagcggg
     5941 gtcattaaat atcaatgacg ttcataatat acgctatgat tattttggtc taaatcattt
     6001 tggctggttt acttcaatag attataaaca tagagactta atggaagaga ttaaggaata
     6061 cataaaagaa aataaaatat tattaccaaa atcttatata gaaaacttaa ataaaagtcg
     6121 tagtacagga aatcgccatg ctgtaagtag ctggatacat gtttgggaat ctgtttatac
     6181 aatgttagaa agcttcccaa aatatctgcc aaacacttat ttaacatact atcttttaag
     6241 taaagaaaca gtggaacatt caaacataaa tcgtactcgt gcaaatgagg ttatggaaag
     6301 ccgtgaaaaa gacttatttg aaggtgtgag acattactta gaaactggag aaatttctga
     6361 aaaagcattt tatgcaggtt cacatggaga ttggattagt gatttggcag tttcaattaa
     6421 aaatgataca cgtagtcgtt ttttagttat aactgaaaat cgtggagcta taccaaacat
     6481 gccatatgat gcaatggtag aaattccagc atatattggt aaagatggtc cagaagttat
     6541 tgctcgaggt ccaatacctc tatttcaaca aggattaatg atgcaacaac ttaacagtga
     6601 aaaattgata gtagaagctt atatagaaaa tagttatgaa aaagctttac aagcattcac
     6661 attaaataaa actattccaa gtatgaatgt tgctaaagaa attttggatg aaatgataga
     6721 agttaataag gaatactggc cagaattaag atagtaggat aaaggaaagg gtttttattt
     6781 aataattaga tatgttttca gagtctttta tcaaaatgta ttcacaaaca tatggcaata
     6841 tattaaaata aagagtgata tttttagaag aatggggtga tgcaatgaac attataaata
     6901 aaattttttg ggaagaagat aggattggca tgattacaaa taatttagag gcagatacac
     6961 atagccactg tatgttacaa ctgttcttag ggatagaaga cagcattgaa ataacagtca
     7021 atgaaaaatt agttaaatgt aattgtatca tagtagataa aaatatctct cattcatttt
     7081 cagcaagaaa aaaagtttat tactctgcta ttattgaacc tacatctata tatgcagaac
     7141 agcttacctc aaaaatgaat gattttggct actggatatg tgacaatgat ggattagaga
     7201 aattacgtca acaaggaact tttttgatag ataactctag taaagaacaa tatttaagat
     7261 ttatggaaat gcttaataac tatttgaata tacctataac tctcaaacat tatgatgaca
     7321 gaattactga attgctcaat ttattacgta cttgtaattg tgataatcat actatttcaa
     7381 gttttgcaga taaagtgtcc ctatcagcaa gtagattatc acatctgttt aaagaacaaa
     7441 tagggatacc tttaaaaagc tatatcttat ttcatcaaat ggagtgtgct tttagagaat
     7501 tactttctgg gaaaaatgtt accgaagctt ctatgactgc tggattcgat actccatcac
     7561 actttgcagg gacagttaag cgaatgatgg gaatgcctgt ttctttatct ttgaaagata
     7621 gcgaattttt gaaagtatat taaatattct cgttatataa tgaagttgta ctaagaaagg
     7681 ggctttgtta tgaataaaga atatttagag gaaacaaaaa tgttaaatta taatgaacca
     7741 caacttaagc ttttggtatc atcaaagaat tggttggaat tagatgattt tcataaaata
     7801 aaatctattt atgagtttgt acagaatgat attttatttg gatataatgc ttctgatatg
     7861 ttaagcgcaa cacaggtatt aaatgatggt atgggacaat gcaatacaaa agctactctt
     7921 ttaatggcat tattacgtgc agtgaatatc ccttgtaggc ttcatgcttt tgatgtaaca
     7981 aaagattttc aacgaggagc tacatccaaa ttgatttcac tattagcacc aaaatatatt
     8041 cttcatactt gggtagaggt attttatcaa gataggtgga ttgctcttga gggtgtcata
     8101 actgataaaa aatatttaga agcaattcag aagaaatttt ttaatcatgg tggaacattt
     8161 aaaaagtatg caatagctac aaatgatttg aaaaatacat ctattgattg ggatggtaaa
     8221 gatactttta tccaaaaaga agctatcgtg tatgattatg gtatttttcc atcaccagac
     8281 gtattttttt ctactcattc acaacatatg tcaaaattaa aaaatttcat atatgtacat
     8341 cttatcagaa aaataatgac aaaaaatgta tgtaaggcta gaaacaatta tattgataaa
     8401 aacgaatgag tgtttaagag gtaactatat aggaaatata tagaataatt aaaactgtac
     8461 tggttaaaat gaataaaaag gagttatcac agagcaaact taaattgttc ataggataac
     8521 tccttttctg tattaacttc taataatttg atgtcttttt ataaaacata tagatacaat
     8581 taaaccagta aatgcaagag tagctgctat tgaaaatgca acatctattc catgaatgtt
     8641 agacagcatg tttgaactca tatgagcttt cttagtagca cttgtcatta tagtaataag
     8701 tatagcagaa cctatagctc cagatatctg acggagtgtg ttattaattg ctgtggcatg
     8761 agatataagt tctctatcta atgttttaat tccccatgta gttaatggca ttaaaaccat
     8821 tgaaatacca atcatacgaa tacaatacat caatgacaca tgaattaaag atgtatctct
     8881 gcctaaaaaa gcgaaggata aagttcctaa aaacaaacat cctgttccta atattgatag
     8941 agcatgaggt ccatatttat ctaaatgacg tcctgcaatt ggattgagta caaccatcaa
     9001 tatagcacca ggtaacatta atgaacctga agataatgca gaaaatcctc ttacagattg
     9061 aacgtacaga ggaatcatta atgtagcaga catcattgag gcatatacaa tcataattaa
     9121 tattgtagaa ataagaaatg gtttattttt aaaaaccctc aattctaaaa aaggttcttt
     9181 agattttaat tgacgcaatg taaataaaat taatgacata attcctatta atattggtaa
     9241 atatgtggca atattagtcc acccataatt accttgatta gtaaatccaa ttaataaacc
     9301 accaaaacct agagatgata gtactattga aggtatatct aattttagtt tttgagtttc
     9361 cccaacattt cttaatagaa taaatgatag tataacatct aatatagcaa taggggaaag
     9421 aaacagaaat aaactatgcc aaccaaatga atctactaac catcctgata aagttggacc
     9481 tattgctgga gcaaatccta ctgtaatacc aactaaactc atagctgcac cacgtttttc
     9541 ctcagggcat aaatagagta taataacttg taaaagtggc attagagaac cagaacccat
     9601 tgcctgcaac acacgagcaa tcaacatagt tgaaaaatta ttagaaaaaa gtgcaataat
     9661 acatcctata gaaaagatag acattgatgt aataaataac tttctagtac taaatcgatt
     9721 tattaaataa gctgttgtgg gtacaataat acctgcaata agtagataaa ttgttgttaa
     9781 ccactgacca atatcagcag taattttaaa atctgacatt atattaggta atgctgttga
     9841 caatagtgtt tgactgagag aacttataaa agcaccagtc attacaattg ctattattaa
     9901 atttcctttt ttattgttac tttgggtttc cataatttat aacgtctcct tttttaattg
     9961 atttttaatg ctgttcaata aaagtacttt tggtcttaaa gtgtcttctt tttctaaata
    10021 tgcaatcatt gcatccataa ttaaagaatc atattgttga ttttttcctt cgacgtataa
    10081 tttttttatt gcttctagta atttaggatt ttcaaggaga gaagtatcag ttttgattag
    10141 ttcttctata tcttgtatcg ttttaaaaaa tagcgattta ttatgaaggg aaaagtaaac
    10201 ttcatcttct tgtttatgta aaaacattga tttttcaatt aaaggattta gattatccat
    10261 attttctaca actaaatcta gaatttgtat agtaaatttg gcaataaagt atacatttat
    10321 ttctcttttt tgtacatgaa taatgtatat aaactgatga attagagcat ccaataagaa
    10381 aactaaatca aatataattt tttctatttt atcaccatag ttatcaaata agcttagttt
    10441 gtattcattc aataagttag ctcttgattt tgtttgcaat ttacttgtag ccttacgttg
    10501 ctcttcagaa aagttttctc ttatataggt atttatggta gttctagaaa aaatattttt
    10561 ccatacagca attatctttc tgtttaactt ttctgatgat ggaatgtcat tattttcatt
    10621 tatggaatgt ataatttcta aaaacttttc atcataataa ttgattattt cgcagataaa
    10681 actttcctta gtttcaaaat gcttgtaaaa agtagcttta gacattttac actcttttac
    10741 aacatccgat attgaagtat tttgaaaacc tttttggtca aaaatttttt gtgcaatagt
    10801 tataatgagc ctttgttttt ctgtcacttt attatctcct ttcacgcaaa aacgtttagt
    10861 actatatagt acctacaagt tttgataaat atattataca tcatttggaa aaaaaatcaa
    10921 tatatagact gtaatatgaa atttttgaaa aatttgttgt tatttttagt cgtataaaaa
    10981 agaaacttaa gagtttctta agaattgttt aaagaagttg caataaatta gtggtatgat
    11041 tatagtatga aagagaatgt cttgaaattt ttctgtatca aattccaatt atggaggtgt
    11101 agtatatgaa aaagaaaatt tgtgtagcaa taatttttgc tctatttata gtttttgtag
    11161 aaatgtttat tgatagctct agcgaaggaa gtaaaattat gacagctgaa ttaagtaaat
    11221 acaggaaagg cgtaaatgta ttatcaataa acaatattat agtagcgaat aaaaaatata
    11281 gtttgcccaa agattattct cctcaggaaa gcagtgaagc gagagatgct ttttataaaa
    11341 tgaataaaga tgctcaaaag tcaggattaa atttaaaagc ttttagtaca tatagaagtt
    11401 atgaatatca agatagatta tttaagtcat atgtaaaaga acatggagaa aaagaagcaa
    11461 atagattttc agctaaacct ggagagagtg agcatcaaac tggacttgct tttgatattg
    11521 gaggagatga ccagtcttgt tgggcaaata aaaagttcaa caatacaaaa gaagctaagt
    11581 ggttgtatga aaatgcatat aaatatggtt ttatacttag atatccagaa ggtaaagaga
    11641 atataacagg atatatgtat gaatcttggc actatagata tgttggaact gaacatagca
    11701 aaaactttgc aatgaataat ttgactttag aagaatattt acatataaat taagagatgg
    11761 tttgttaaaa ttaggttaaa ataaaaaata acctagacta ttagctgtgt aaagtgatat
    11821 aatgtaaata caatttaaga taactactcg aaggggagta gcttacacga aagtggtaat
    11881 taatcgtcaa gacagtagaa atactcggtt aattagcaag ggattccttg atagcaagac
    11941 cttcaaaata agcattaggt atgtttgttt tggggtcttt ttttattcaa ggaattttta
    12001 tttaaaaaaa tttaaatttc aagtatacat aagagccccc aaacataaaa taaatttatt
    12061 gaagaggagg gcaacaaatg tcttacaaaa gtacttatca agaagtaatt aaaaatttag
    12121 actcaagttt atctggtttg agtaaagaaa gagcagaaga gttgttagaa aaaaatggag
    12181 ctaatgaatt aaaagaagca gataaggttc ctacttataa gcttttccta gaaagtttta
    12241 aagacccatt agtaataatt ctattgatag ctgctttagt acaaatattt ttaggtgaaa
    12301 cagtggagtc tataataatt tttgcagtta ttattataaa ctctgttctg ggtgttgtac
    12361 aaacaaaaaa agcagaaagt tcattagaaa gtttgaaaaa tttatctgca ccaaacgcaa
    12421 aggtaataag agacaataaa aaaatgacaa ttccagcaaa aaatctagta gttggagata
    12481 ttgtattctt agaagctggc gattacatac cagcagatgg aaggcttata gaagcacaat
    12541 ctctcaaggt tgtagaaggg atgttaactg gagaatcaga accagtttta aagcatatag
    12601 aaaaaataag tgaagatgta agtttaggtg accagaaaaa tatggtattt agtggtgctg
    12661 tagttgttta tggtagaggt tcttttgtag tgactggaac aggtatgaat actgagatgg
    12721 gtaaaatagc tgggctttta gaaagtgctg aaaataaact tacaccattg caacaaaaaa
    12781 tagaggattt tagtaaaaaa cttggtgtag gtattgtaat acttgctctt ttaatattta
    12841 taattcaagt agcaagaaat tactttatgg ctggaaatgg agaaatgaca caaattattt
    12901 tagattcatt catgttctct gtagcagtag cagtagcagc tataccagaa gctctatcat
    12961 caatagtcac tatagtattg gctgttggaa caaatagtat ggcaaaaaaa caagccataa
    13021 taagaaaatt accagcagta gaaactttag gttcaacaag tgtaatatgt actgataaaa
    13081 caggtactct tacacaaaat aaaatgacag tagttgatta ttatatgtat ggaactaata
    13141 atgatgagtt agataaagaa aaagtaaact attcttatca tgctaaatta ttgactatgg
    13201 tttctacatt gtgtaatgac tctcatataa ctagtgatgg taaagaagtt ggtgacccaa
    13261 cagaagtggc tttaataaat tattcaagta aaaatgattt agattatgat aaattaagaa
    13321 gtaagtatat cagaataaat gaaataccat ttgactcaga cagaaagctt atgtctactg
    13381 taaatagtat atatggagat tatataatgt ttacaaaagg tgctccagat attgtgttta
    13441 gtagatgtaa atatgctttg aaaaatggtt caaaagtaga tataacagat gagataatag
    13501 aagaatataa acataaaaat gaagagttct caaatagagc acttagagta ttagcttttg
    13561 caataaaaga tgtcccagaa gaagatttta taccttcaat tgatgacgaa catgacatga
    13621 cattggttgg aataatggca atgatagacc caccaagaga agaagtaatg gatgcagtaa
    13681 aagaggcaaa gagtgctggt ataaaaactg ttatgataac tggtgaccat aaaacaacag
    13741 cagcagcaat tgcaaaagag attggtataa tggaagaagg agacttggct cttactggaa
    13801 aagagcttga tgctttgagt gaagaacaac tatatgaaaa actagaaaat ataagtgtat
    13861 atgccagagt ctcgccagaa aataaaattc gtatagtaaa agcatggcaa cataagaata
    13921 atataacagc aatgactggt gatggtgtta atgatgcacc tgcattaaaa caagcagata
    13981 taggaatcgg tatgggaagt ggtactgatg tagcaaaaga tgcttctgct atggtattgg
    14041 ttgatgataa ctttgcaagt atagtaaatg ctgttgaagt tggtagaaca gtatacagta
    14101 atataataaa atcaattact tatctatttg caggaaactt aggagctata gtatccatag
    14161 tatttgcagt gtttgcaggt tggtcaaatc catttacagc tttacaactt ttgtttataa
    14221 acttagtaaa tgactcttta ccagcaattg cattagggtt tgaaaagtca gaaaaaaata
    14281 taatgaataa acctcctaga cacccggatg aaagtatact agcaggagga acattgaagg
    14341 cagttgctat aagaggttgt ataataggtg tagttacaat aattgcacaa tatataggat
    14401 tacaagtatc tccagaactt ggagtagcaa tggcattttc aacattgata ttagctagaa
    14461 taatgcaaac attacctgca cgttctaata atcaaacagt aataaaatta ggttttttca
    14521 gcaataaata cgttatagga gcagttttag tctgtcttgg gctttactct ttaacactaa
    14581 taccagtgct aagaggaata ttctctatac caacaacgtt tggattgtac cagttgggga
    14641 tttgcctagg gttagcatta atttcatcaa tattaatgga acttaacaaa ttaataaata
    14701 gaagtgaagt aaaataatca aaaaattaaa gggcatattg attttaacaa tatgcctttt
    14761 ataaatagtc taatttattc tatattatcg attatttgga atgcttttat aacttcaggt
    14821 aattcttttt cttcaggcat tctaaatctt atgaaatttt tatcaagtcc agtaaaatca
    14881 cttccagata taactcttat ttttaattta gcaaactctt gttgtaaatc tatgtccttg
    14941 tttttatgtt caactgtcat aattgataca gtatcactag tttcagctat attaaggttt
    15001 ttccatggaa ttaataattg attttttata tcttttgttt tttcttttaa ttcttctata
    15061 aattgaacat catctaataa atttgctgca atactacgag atatttcaga cacttcgtaa
    15121 ggagtagaaa tctttttaat agggctaaca agttgttctg gaagaactgc atatcctgct
    15181 cttagtcctg ctaaaccaaa tccttttgaa aatgttttta aagcaattac attatcataa
    15241 ttatttagaa gcttgacagc tgaattttct tttggcatgt attcaccata agcttcatct
    15301 accataactg ctatatcata tttagctgct tcctttacta tattttcaat tgatgataaa
    15361 ggtataattt gtccagtagg attgtttgga ttgtctatgt aaattacttt atattctgga
    15421 tttatttttt caataaattc tttttcatta aacttgaaat tatcctcttt ctttaaaaga
    15481 acataatcat aagttgcacc atgcattttt atatctgtct catattctga gaactgaggt
    15541 gaatatccta aaactttatc tcccttttca ataaatagtc tatttaaaag gtaaataaca
    15601 tgtatagaac catctgccaa acaaattctg tcagtatcta atgctataaa atctttccaa
    15661 taatcaacta tattatcttt taaagcaatt gaatgaggat atcctcttat catatcaaat
    15721 tttaaagcat taaaagcttc tacagctttg tttgagtaag acacggtatt tataccttca
    15781 ccacagtcta tctcgaatac atcttctgaa tgtccagatt ctattgcgta actttttttc
    15841 aactgcattt caatagcttt gttaattttt agtttcatac tttatcactc cttatttagt
    15901 caataataaa tgttataaaa gttgctagat ttaaaatatt atgaaaatat ataatagtaa
    15961 aaaatgaata taaatattat aatagctcac tttatttaat atgattatat atcatatatt
    16021 gtaaaatttc tagcattata agactataaa ttgaaaatat tcagaaaaat actacaagaa
    16081 acgatttatt ttgctcttaa aataagttaa atgaatgtta ttaattttgt aaattgaaat
    16141 agtttaagta taaagttatg atataatcta ataagggtaa ttctttagat tatattaagg
    16201 aaatataggt atacaataaa aagttatgga gttggattat gggatataaa ttaattgtaa
    16261 cagatatgga tggaacttta ttaggcaata atcataaagt tacagatgaa aataaaactg
    16321 cactacaaaa agttataaaa agcggtatta atgtaacttt agcaacagga agagccttcg
    16381 attcagctaa atgcaatgta gattttctta aagaggacat gccaataata gcttgtaatg
    16441 gttcattaat aagagagcaa aatggaaaca taatatattc aaataagatt gatacaagaa
    16501 catgtttaaa cattttagat gtattagata aatacgatat ttactaccaa tgtaatagta
    16561 tagattctat gctttcgaaa aaaatagaag gacgtgaaga tagattaagt gtatttttag
    16621 gctctgaaac agaagtaatt gtaaaagatg atttgagaga agaaatcttt aaaaaagata
    16681 tactaaaatt tgtaatcata gaagaaaaaa atccatctat tttggatgaa ataagaaaag
    16741 aattgagaaa agttcaagga ataaagataa cttcttcatg gccaaacaat attgaagtca
    16801 tgaatgaagg tgttgataaa ggtaatgcag ttaaaatttt agctgaaaaa atgaatatag
    16861 acagagaaga tataattgca tttggagaca attacaatga catagaaatg ataaagtttg
    16921 ctggtcttgg ggtagctatg ggaaatgcag aagaattgat taaacaagaa gctgattatg
    16981 ttacagatac aaatcaagac agtggagttg caaaggcaat ttataaattc ctagagttaa
    17041 aagaatcact tagctcttaa aacagtatat aataggaaaa aataggttga ctattttgta
    17101 gatagtaatt ataatacaaa tagataaaac ataaaaattt agattcagtg aaaaagagag
    17161 tagatataga gaaaatctca gcgaattggg ggatggtgtg agcctaatga taacacttta
    17221 tattgaaaag aactttggag aatactactt gaaattcagt atggtagtag gtaatctcgc
    17281 cttaagagaa tagagtgggt aaacattgtt taccaactag agtggtaaca cggaatattt
    17341 cgtctcttgg tctttttgac caagagattt tttagtttca ttgagttaca agacttataa
    17401 ttaaaataca aaaaattaaa atatgaggtg aaagttatga aattatgggg cggacgcttt
    17461 agaaaagcgg aaaatcaact tatggaagaa tttaataaat cttttggcta tgattgtgta
    17521 ctgtacaaaa aagatatcga aggaagtgta gcacatgttc atatgcaagt aaaatgtgga
    17581 cttttaacag aagaagaagg aaagtctata acagaaggtt taaaagggat acttgaagat
    17641 gtagaaaatg gaaagttagt attggatgga gagtatgaag atatacatag ttttactgag
    17701 ataaatttaa ttcaaagaat tggagatgtt ggcaagaagc ttcatacagc acgtagtaga
    17761 aatgaccaag ttgcagttga tatgagattg tatgctaaag aaaaagcaaa tgatttggtg
    17821 ggtttaatat ctgaatttaa agctactata aaagatgttg ctgacaaaaa cccagtgatg
    17881 atgccgggct atactcatct tcaaagggca caagtagtga catttaagca tcacttaatg
    17941 gcatattata gtatgtttga tagagatgag aaaagaataa aaaatgctat agaaatatta
    18001 gatgaatcac ctttaggctg tggagcttta gcaggaacta cgcatgatat agatagaagt
    18061 ataacttgtg aacaattagg atttaaaaaa gtagtagata attttatgga tggagtaagt
    18121 gatagagatt atttactaga gctaatgagt gatttttcaa ttataatgat gcacttaagt
    18181 agattaagtg aagagttgat actttggagt tctcaagaat ttggatttgt tgagatagat
    18241 gatttatata caactggaag tagtataatg cctcagaaga aaaatccaga tggtgcagag
    18301 cttataagag gtaagactgg tagagtatat ggaaacttat ttggtctttt tacagtaatg
    18361 aaaggaatac cattagcata taataaagac atgcaagaag ataaagaagg ttttttcgat
    18421 agtgtacata ctcttgaaat gtgtatacaa ataatggata gaatgatagc cacactaaag
    18481 gtaaatgagg ataaaatgaa acaagcagta aaaaatggtt ttttaaatgc aacagaagtt
    18541 gcggattatt tggttaaaaa taatgttgct tttagagatg cacatggtat agtaggaagt
    18601 atcgttatat attgtgaaga taataaaaaa gcaatagaag atttaacatt agaagaatta
    18661 caaaagtttt cagatgcttt taaggaagat atttatgatt ttatcgacta tgagagtata
    18721 ttaaataagg gaataaaaaa gaatttaaaa taatgtattt atttttaaaa gctgtaaatt
    18781 tgaatgttat catttaaatt tacagctttt tattgtgtaa gattttatta tataaaagta
    18841 agaaacttaa aaggagtagt atttaaaata aatacaaata aaaaattatt attaaaatga
    18901 taattaatat caataagtat tgacaaaatt atttctaaat actattatat ataatataaa
    18961 taataatgaa aatcaatatc aaataaatgg gaggaaatct aatgaactta ttagtgattg
    19021 gtggtcatga aagaatggaa aaagattata tggttatggc caagaaaaaa ggttataaga
    19081 caaaggtata tactaccatg tcatctaaat taaataattc tataggaaga ccagatgcag
    19141 ttgtcatact tacatcaact gtttcacata agatgtctag aacggttgag tctcaagcta
    19201 aaaaacaaga tatattaatc gtaaggcata aaaatagtag taaggtagca tttaatgaat
    19261 gtctagatat ggttgatgaa tgtctgggaa attgttcaaa atgtaagctt aaagacaaac
    19321 aaaaacaaaa aaatatatag gtataaaaaa tacataaaca taaaattaaa agtactgaga
    19381 taatagttat aattattatc tcagtacttt tagatttata aggataagta tagtataaat
    19441 tttttattat aatttccaac tacttgttac tttttcatat tttttttact tctagctttt
    19501 tttctttttt tcttctttat atttgaagct attataaata aaactaaaag cactagaata
    19561 cctgcaataa tcttaacaaa agtcattaaa acattatttt cagtcaaaaa tggaagactg
    19621 ctatcaagat tattttttgc aattaagtct atactgtttt ctagtttacc attattatat
    19681 acatttaaag taccaacctt agcccctttt tttataggca aatcaatttt atttaactct
    19741 gttttggtat cataattgcc ttgagattca tctttttcta agaatatcct ataactattt
    19801 tttggttcat aaatcaattc atgttgtttt gtaaacaata cttttttagt atctgtgtat
    19861 ttttctttat caacaatagt agcacactta aagttatcaa atccataatc aatcagaata
    19921 cgtgagtcta aatataaatc agcattagtt gaattaaata cagcaacgat aactcttctg
    19981 ccatctttta cagcactcga taaaagacat ttaccagcag catcagtata tccagttttt
    20041 ataccatcaa ctatatcata ttttatattt ataggttgac ctttgtatgt aatcttatca
    20101 tgtgatgtca aaaacttatt agtgtttaca aagtatctct cataaggata tgcttttgta
    20161 gcttcaaatt taagtgactt agtttttaca atttgtctaa aagtatcatt attcatagct
    20221 tctctagcta tgagagccat atcatgagct gttgtgtagt gatttgggtc tggtaatcca
    20281 tgaggattat taaaatgagt attagtagct ccaatttttt ttgcctcaga gttcataagg
    20341 tcaataaatt tttgtacatt gccaccacca acatatctag ctaatacaaa agcagcatca
    20401 ttagcagaat gaaccaaaag agcatctagt aactgcttta cagtaaaaga ttccccattt
    20461 tttagataca tactagaacc atctatttga ggtaaatctt taatttctat tacttgattt
    20521 aaatctttaa cctcctttag aactaaacaa gctgtccata ctttagttgt tgaagctgga
    20581 taaagcttag aatttccatc cttattatat agtattttgc cagactcata gtccattaaa
    20641 actgcatgtt tagcaactat agctggttca ttatctgcaa aagagatatt agagcttcct
    20701 aaaaatgaag taaatatcaa taaacagatt aataaaagtg ataaatttct tttcattgtg
    20761 agcctccaaa ttttaaattc atcttttagt atagcatata tttgtcaata attaaaagaa
    20821 aggagtgatt aatatatgaa aaaaaaggta aaatttattt cgttttttgt tttatttatt
    20881 ttaataatat catcaataat cgtttataaa tcgcttattt tcaaagatga tatttatgtt
    20941 gtaagtaaaa aaaatttacc taatgaaaaa acacatgttg tagagaatga aattaaaggt
    21001 gaagataata atcacagtaa agtaactaaa gacatagata aaaattttca aaataataaa
    21061 ataactatat acataagtgg agcagtaaat agacctggaa ttgtaactat agagtcagac
    21121 aagagacttt atgatgctgt agaattacta ggaggaacga caaaagaagc agatttaaat
    21181 ggagtcaatc tatcagtaag attagaagat gaacaacatt acataatacc taaaataggt
    21241 gaagcaacat ctgttacaag taatgatgat tccaataaac caaatcagaa gaatgagtca
    21301 agaggtgagc ctaaaaataa atccaataaa aatatatcta acgaatcaaa agtaaatata
    21361 aatgtagcga ctatagaaga gttggattct ctacctgggg ttggagaagc tacagccaat
    21421 aaaattttac aacacagaga agaaaatgga cagtttagtt caattgagga aataaaaaat
    21481 gtaaatggta ttggagataa aaagtatgaa aatataaaag atttaatttg tgtagactaa
    21541 aatataatac aaaacttaca tatagatatg ttattataaa tatgtatgta tatgtgtgta
    21601 catgtggaaa tttagtaaag gaggcacttt tatgaaaaaa gaacaaaaaa aacttacaga
    21661 gatgacaaca gcaggtggtt gagccgctaa aatagggcca gaggttctgg catcagtttt
    21721 atctcaattg cctaaaaatg ataatataga aaatttatta gtaggactag atacagcaga
    21781 tgatgctgca gtttataaat taaatgatga tatggcactt atccaaactt tagacttctt
    21841 tacaccaatg gtagatgacc catacgtatt tggtcaaatt gctgcatcaa attcactatc
    21901 agatgtatat gctatgggag gcaaaccttt ggttgctatg aatattgttt gttttccatc
    21961 atgccatgat atggatgtac ttgcagaaat attaaaaggt gggtttgata aagtgaaaga
    22021 aagtggagca cttttggttg gagggcatac tgtagatgac aaagagccta aatatggtct
    22081 ttcagtttct gggattgtaa gtccaaataa agttttatca aatgctactg caaaacctgg
    22141 tgataagtta attataacaa aaccaattgg agtgggtgta ttaaatacag ccatgaaaga
    22201 aggtatggta gaacaacata tagcagataa agtaatagaa ataatgatac atttaaataa
    22261 atatgcagca atgagttttg agaaatttga tgtaaattcg gttacagata taactggttt
    22321 tggtctactt ggtcatactt tagaaatggc caaagcttct gaagtaagta ttgaaattga
    22381 atcaaaacat gtaccaatta ttgaaggtgc aatagaaatg gcacaaatgg gaattatacc
    22441 tgctggtatg tataaaaata tgcattatgt atctaaagat gtagaagttg taggaaatat
    22501 agaagttgca gttcaagata ttttatacga cccacaaaca tcaggagggt tgttaatttc
    22561 agtaaaagaa gaacttgcag aggagcttgt aaaagatatg aaattaaatg gagcaataga
    22621 agctaaaatt ataggttctg tagttccaaa aggggaaaaa tatattacag ttttataaaa
    22681 tattatagag aaagagtgat tgttttgagc aaaagagaat tgtttgcaat gttgccttca
    22741 gttgatgaag tattaagtga taatagaatt gtagaaataa taaatgaata tcctagaagt
    22801 ttagttttag aatctgtgag agaagtaata gatttaaaaa gacaatttat attaagatta
    22861 aaagaagatg catcaaactc tgtaacaata gagtttggtg aaataataga atctgcaata
    22921 gagagagtta agttaaatta ctcattatct ttaaaaaaag ttataaatgc aactggtaca
    22981 gttattcata caaatttagg aaggtcttta cttagtgaag atataaaaga tgaattatgg
    23041 tgtgctgctt caaggtattc aaacttagaa tacgatttgg ataatggaga aagaggttca
    23101 agatactctc atttaactag tacaataaaa agacttactg gagcagaaga tgtactagtt
    23161 gtaaataata atgcagcggc agtacttctt gtacttagca caatggctaa aggaggagaa
    23221 gcaattgtat ctcgtggaga attagttgaa gtaggtggtt catttaggat tccaagtata
    23281 atggctctta gtggtgcaga attagttgaa gttggttcaa ctaataaaac tcatttgaaa
    23341 gattacaagg aggccattac agaagataca aatgtattga tgaaggttca tacaagtaat
    23401 tatagaataa tgggattcac agagagtgtt tctatagaag aattggttaa tttaggtaaa
    23461 aaatataaac tacctgtaat agaagactta ggaagtggag tttttataga tttatcaaaa
    23521 tatggtttat cttatgaacc aactgtattg gattctattc gacaaggtgc agatgtagta
    23581 acttttagtg gagataagat gcttggaggt ccacaagcag gtattatagt tgggaaaaaa
    23641 gagtatatag aaaaaatgaa gaaaaatcaa cttactagag cacttagagt tgataagctt
    23701 acaatatgtg ctcttgaagc aacattgaga atgtatttag atgagacaaa agctatagaa
    23761 aatataccta ctttaaaaat gttgacatat aaaattgaag aattagaagt aaaagcaaat
    23821 aaactatttg aaaaaataac tgctttaaac ttaaatgcaa acataaatat agaagatgga
    23881 ttttcacagg tgggaggagg gtctatgcct cttgaaacta taagtacaaa ggtaatatca
    23941 ataactcctg aacatatgaa tgtttcatct ttagaaaaaa aactaagact tagtgaagca
    24001 catataatag ccagagtgta tgataataaa tatgtactag atgtaagaac tatatttgat
    24061 gatgaatttg atgtaatagt tgaggaattg agaaaagcat ttaattaaat aaatttgagt
    24121 taataaatat taaatttaga agtataagta tgaagttttg attaaaaaat agctacacac
    24181 acttgtgact ttagtcatag caggtttaga gggggcaaaa aatgaaaaat gtagtaattg
    24241 gaacagcagg tcatattgac cacggaaaaa ctactcttat aaaagcattg actggaagag
    24301 agactgatac gttagatgaa gaaaaaaaga gaggtatttc aataaactta ggatttactt
    24361 attttgattt accaaacaat agaagagctg gaattgttga tgtcccagga cacgaaaaat
    24421 ttataaaaaa catgttagct ggagcatcag gaattgatat agttttactt gttgtagcag
    24481 cagatgaagg ggttatgcca cagacagttg agcatttaga tattctaagc tttttaaata
    24541 tcaaaaatgg aatagtggtt ctaactaagt ctgatactgt agatgatgaa tttagagagc
    24601 tagtaaaaga agatatttgt gaaaaaataa aaggaacttt tcttgaagat gctgaaataa
    24661 ttgaagttga ttctatatct aaaagaggta tagacactct tattgaaaaa atagataaaa
    24721 tgagtgatga tatagaagat aaaaatgaaa actctccagc aagacttaat atagacagag
    24781 tattttcaat aaaagggttt ggtactgtaa taacaggcac tttaatagaa ggaaagataa
    24841 gtatagaaga tgatttggtt atatatccac aggtattaaa aacaaaaata agaagtatac
    24901 aagttcatgg agaaaataaa gatacagctt atgcaggtca aagaacagca atcaatatat
    24961 caaatgtaaa agtggatgat ataaaaagag gagatgtttt agctgcacca aactcattgg
    25021 aggaatcaat gatgttagat gtaaaattat cattggtaaa tcatttagat aaaggattaa
    25081 agcactggga tagattaaga ttgtatcatg gaactagaga gatactttgt agagcagttc
    25141 cactagataa agaattaatt gaaagtggag aaagtggcta tgttcagttg agacttgaag
    25201 aaagcatagt atctaagaaa ggtgatacgt ttgtagtgag acgttattct ccaatggaaa
    25261 ctataggtgg aggtgtaata attgacccat ctcctaaaaa acataagaaa tttgatgaaa
    25321 aagttataga agcattaaaa ataaaagaaa aaggcgaact aaaagatata atagaagagt
    25381 acttaaaaag gaatttaaaa aattatccaa atataaaaga aataatgagc tatagtggtg
    25441 ctcatgaaga agatgtaaaa agagcgcttg aaacacttat atcagaagat aaagtattta
    25501 ttataggaaa tatgtatatg catataaatc aatacaataa attaaaagaa aatacaataa
    25561 agcttttaag tgagtaccat aagaagtata ggcttagaaa agggatacta aaagaagaag
    25621 taaggtctaa gatagagagt aattttaaga ctagggagat ggatatttta ttagaaaaat
    25681 tgtctacaga aaatgctata aaaatagaaa ataatatagt atctctatta gattttgagg
    25741 ttattttaaa tgataagcaa aaagaaatag caaaaaaaat agaaaaaaga ttaaaatcat
    25801 gtggagtttc ttctatactg acaatagatg aagtttctga aggaaatcat aactatgctg
    25861 aagtattgga gtcaatgata ggtaataaag tagagaaatt agatgatttg tatataatgg
    25921 acaaagatat ttatgaaaat gcaaagaaca ttttgataaa ttatataaaa gaaaataaag
    25981 aaataacatt aggagaatac agagatttga ttgattctag cagaaagaat tgcatgatta
    26041 tattggaaaa cttcgataga aacaaaataa ctaaaagagt agaaaacaaa agaattttgt
    26101 tttagtagaa aatgtctgtt tttgctttaa ggtataaaga aggaattata atgtcgagta
    26161 tcgtataatt aattagtata aaatattaat taagaggaag tgtaaagtat tgaagaatat
    26221 aatacctgat gtaaaaccag atataaaaga aagagaagac atgagccttt tatttataat
    26281 aatgggctca gtacttttat tatttattat aaaagatata gagaaatatg aaactttatt
    26341 acatgccata tatacgatat ttttagtagc tacaggagta attatattta atactacaaa
    26401 atttaaagta gattcatttt ctacattttt aggattaatt tttatagcta ctggagtatt
    26461 ggagtgtata tatttattta attccttggg gattaaaact aagtctataa tggagattaa
    26521 catcacaata tcagcaataa cagatttatt tcctatactg ggtgtatact taagttttaa
    26581 atttgttaag gataacaaac aaatttattc aagtgtaatt ttatttatag caactgctac
    26641 attaacaata acatctttat tcataatatc aggaatttgt gactatctgg gaaatggtaa
    26701 atcacaatat gctttaggag ttataatatc tatattcata ataattattt ctatcgttag
    26761 tggaattgaa ttaaattcaa gttcaaataa aggtaaatgg gaatatgatg aaaaaaagat
    26821 ttttaacaga ataatagtaa taataatctt atcaagagtt ccaaatctat tgcatatagt
    26881 tattgataat agaaatgtag agaggatatt atcacaaata ataataaatg tagccttata
    26941 ttatttatat aattacatag tatctaagaa tattaagaaa acagcactgg aattgcatga
    27001 tacgaatgaa gaattaacaa aaaaaacgga aagtttaaaa gaaaagaata aaaaattaat
    27061 ctatgagaca gaaaagatag aggaacttaa gggaatactt atgaaaagag aatctaggct
    27121 acaatctaca ttagatgtag cagtaaatag catagtagta tttagcaaag atggagaaat
    27181 tacatatgca aataagagct ttagaaatac ttttggagac tataaagaac aagataaatt
    27241 gactgataaa ataaaaaact tcaatgattt gatagaaagc attcattatg tgtttgttca
    27301 tgagaagaat gcagagaaac ttattagtac aagcgataat aaagtttatc aagctatatt
    27361 tgcaccttta attattgctt cacaaacaga aggagtattg tgtgtactag aagataagac
    27421 taagaaaaag gagtacgaac gcaaacttat tgaagcaaat aagagaagta aagatttttt
    27481 agaaagtatt ggagatggca tagtagtgct tgaaggtaat aacaaaatat atgttaataa
    27541 tgcatgtagg gaaattttta aaaataattt agagagtatt gatttttcat tagtatgtaa
    27601 aagtgaagaa agtatagaaa aaagatatgt aatagatggt gaagtaaagt atgtagagat
    27661 gagtttttct cagtatacta atgctggtac taataaaact attattgtaa tacgtgatac
    27721 aacctctcga aaaatatctc aaataaaact tgaggaaagt caatcatcat attcaagatt
    27781 tatagatata ttacctgatg gcatatgctt attaaaagaa gatttaagta taaactatgc
    27841 aaataagtcg tttttagata tgctagcttt tacaaacata gatgatataa aagattctaa
    27901 tattaaacta ttaatgaatg cgaatactga agaaaaaatg aaatttacgg ataatatggc
    27961 taaagtttta aatgaaaata gaagtatact tttactggaa tatgaattga taaatagtta
    28021 tgatgatata gtagaagtag aattaagtgc attacctttt gctatataca atactagata
    28081 cataatgctt attataaaag atttagtaca taagaaatat tccgagcaag cagaaaaaga
    28141 actgcttgaa agatttaaaa cagataaaat caaaacggag ttttttgcaa atatgtctca
    28201 tgaactaaaa actccactaa atgttatatc tagttcaaat caactggtag attctttcta
    28261 tagaaatgaa aagataaaag attacaataa caatattaaa tctcatgtgg atttagttag
    28321 acaaagttct tataggcttc aaagattaat aaataacatt atagatttaa caaagatgga
    28381 gtctgggttc tatacactaa aacttgcgaa atacaatatt gtatctgtaa tagaagactt
    28441 atttatgaat atagaagagt atgcacttag aaaagacata aaaattcttt ttgatacaga
    28501 ccttgaagaa attaatgttt atatagacaa ggttgaaatt gagagaatta tgttgaacct
    28561 attgtcaaac tgtattaagt ttactgataa tggaggctgg atatatgtaa gtattcatta
    28621 taaaatagat aaagtaatca taagtgtaaa agacactgga gttggaatac cacaagataa
    28681 attagaattg atttttgaag aattttcaca agttgataag actttatcca gaaatactga
    28741 aggaagtgga atagggcttg ctatagttaa gaatctagta agtcttcatg gtggagatat
    28801 agaagtagta agtgaagtga ataaaggcac agagttctta atttcacttc caatgaaggg
    28861 atttagtagt gaacattaca cagaagataa gagaatatat aatattcaag aaaaaataaa
    28921 aatagaattt tctgatatat actattaaaa atgattatga gcaaaaataa aataaaaata
    28981 ctatttgttt aaatatttaa gttgtaagaa ataggctatc ataaggtaaa ttattatgat
    29041 agcctatttt tagctaggtt ttatatctat atgtgagaac atataatctc taaattacct
    29101 tcaaaactaa aatctccaaa gtcgtttggt agtataaaat gcataccctt ttttaaattg
    29161 tattctacag aattgtgtat aagttttcca tttccatcca acacactgta taaagaaaaa
    29221 ggtgtattgt gtgtaaaatt acattttcca aaaacatcta atttataaac actaaaaaat
    29281 tcgtttgaca caaatgttgt gcatttaaag tctgatgttg atacgatttt atagtcagta
    29341 tcgaaattta tatgaggaac attcgtaaca tctattgatt tttgaacatg aagttcacgc
    29401 ttattacctg aattgtcagt tctatcataa tcatagactc tataagtagt atctgagttt
    29461 tgttgagttt ctaaaatcaa tgttcctttg catatagcat gtatagtacc acttggaaca
    29521 tagaaaaagt ctcccttttt tatattaacc tttctaagta gtttgtccca ttcattatta
    29581 tttaccatat ttacaaattc tttatgagat tttgcattat gacctattat aatttctgca
    29641 tcatcagaac aatctattac ataccaacac tctgtcttac ctaactctcc attttcattt
    29701 tttttagcat attcatcatt tggatgaact tgaactgata aattatcatt tgcatccaat
    29761 atttttgtaa gtaaaggaaa cttatcacca ggtgtgtttc caaataattc tctattttta
    29821 ttccacaatt cagatagttt tttaccttta tattttccat tttctattaa gcaatctcca
    29881 tttttatgag agcttatagc ccaacattca ccagttgttg gcgaatctat ttcataatta
    29941 aatttatctt taagtgcagt accaccccat attctatcca taaaaatagg ttttaaaaat
    30001 aatggttcca taatcctgtt cctcctatga tattaaattt atcctacaaa taattatata
    30061 taaatagatt ataaaatcaa tagtaaaaaa gttttgtccc ttgaaatgac ataaagtata
    30121 aggagttatt aaataagata aactatgata tataatataa agttctaaat tggagtgata
    30181 attatggagt tgtttaaggc agaaagcaaa aaatatgaaa aagaattttt aaaattattg
    30241 gagcaatggg ttagtatacc ttcattttat gatagaaaaa ctgtatctaa agatatgcct
    30301 tttggaaaag gagtatatga tgcattaaac tggtttgaaa atctaggtag agaaaacaat
    30361 ttcaaagtaa aaaatataga taatcatgct gttcaaattg aatatggaaa tggtaaagag
    30421 tatgtagata tatttggaca ttgtgatgtt gtaaatccag gtgaaggatg ggatagtgaa
    30481 ccatttaaac taaatattat aggtgataaa ttagttgcta gaggtgtatc tgataataaa
    30541 ggccctatga tagtgaattt tttagcacta aaaatgataa aggatttaga tattaattta
    30601 aaaagaaagg ttagattaat agctggggga aatgaagaga gtggatttaa atgtataaag
    30661 cattattata gcaaagagcc atatggtgta tgtggattta ctccagatgc taaatttcct
    30721 gtattaaatg gagaaaaagg tggagcaata ataaagttaa tttcaaatat agacgataaa
    30781 tctttatata taagtggtgg aatagagttt aatacaatac cagataaagt ttatataaag
    30841 aatgtagaaa aattgggtaa agataatata tgttttgata ttaacaatat atcgataaat
    30901 tatgataatg gcaattatat tgtgtatgga aaaggtggtc attcatctaa acccgaaaaa
    30961 tcaataaatc ctatattagc tacaattaaa cttttgtcag aaaatattga tgaaaaatgg
    31021 acaaaagatt tatataagtt gataaatcaa gataatataa atggaaactt atttggttta
    31081 aatattgagg ggaaatgtgg aattttatca atggtgccaa caataataaa tatagtagat
    31141 ggaaaattag aagtagtttt aagcgttaga tatccagaaa tattaaccat tgaagatata
    31201 ataaaaaaat ttaatttgta tatggagcaa aataatatta ataaatttga gcttattggg
    31261 gaaaatttaa agcaagcaaa ttatatagat agaaattcaa aacttgtaag aagtttgcat
    31321 gatatatata taaagtattc tggagattta aaaaatgatg ttagagtaac tagtgcagga
    31381 agttatgctt ctgaaatgaa taattcagta atatttggtt gtgaatttcc tgatggtagc
    31441 tttggcaatg tccatagtgc gaatgaattt gctagcttag atagatttat aactgcaata
    31501 ggtatatatg cagaggcaat aattacatta tgtaataaaa tataaataca aaatgtccct
    31561 tatatagaca tattatatat acaattaaaa ttttacttga tatataattg aaatcaaaga
    31621 gaaggtgata caatgataac taagaaacat gcaataatag taaagagttt aagcaataaa
    31681 gatggatata tgacatcaaa cgaattagct atcaaattgg atgtgtctac taaaactatt
    31741 aaaagatata tagcagattt aaatagtgtt ttaagcaagt atgatttggt aatagcttct
    31801 tctagaggta taggatacaa gttaagcggt tctaaaaaca atatagccag agctgttaaa
    31861 gaagcaaata aatatattga tggtttttta gatgactctg aagagtcaag aatgtcaaat
    31921 ataatatgta tgcttattaa tagaaattat atgagtatag aagcaatggc agaagaatta
    31981 aatctaagta tagccgctat aaataaattg tctagtaagc ttaaaagaaa acttgaaaag
    32041 tatgacttag ttataaaatc taaaccttac tatggaagct atatacatgg tgaagaaatt
    32101 aatataaggc aattaattac tgactatgca ataaaattag atgaaaaaag taaagtgaag
    32161 gtgtttttag atgacattag tgaaaacgat atacactgta tagaaagtat tttagaaaag
    32221 cacttaagag aaaaagatac aataatttca gataaagatt ttaatctttt gctttcaaaa
    32281 ataatcgttt ctgtatttag gagtaagaga ggtcatagta acaatataaa cttaatggat
    32341 acaagttata gatttcataa ctactacttt atagaaaatc taatgaaaga aatatctgat
    32401 aaaattggat ttaaacttat tgaagatgaa gtaatttata tttcaaatta ttctggtgta
    32461 atagcctata aaggtacaca aggaagaaaa aacacaagtg aaattgagga acgaataagt
    32521 agtgttataa gtagtgcact tcaagatata ttccttattt caggtagtga ttatacaaaa
    32581 gatgatgagt ttatggttgc aatctctgac catataaaga gatttttaaa tagagctaga
    32641 gctaatgtaa aatcaaataa tcctttactt catcaaataa aagagaagtt tcctatagct
    32701 tttaatttag ccgtgttttt atcaaataaa ctagaaactg agtttaattt aaaacttgat
    32761 gaagatgagc ttggatatat agctatccac tttgctgctt caaatgaaag aatgaaaaaa
    32821 aatactagta agaaaatttg tatagtttgt cattatggga taggaacagg acaacttatt
    32881 tctgaaaagc taaaacagaa tataagtgat ttaagtgttg tgggtgttta tccagttaga
    32941 tatctcgata ttgctataag tcaagatgtt gatttgatag tatctaccgt tgaattaaag
    33001 ggatatgaaa aacctgtctt atatatagaa aatatatttg atgatagctt aattgaaaac
    33061 gtaaacaaag ccttttatga aaaggaagaa agaagaaaga taataagcaa catgtttgat
    33121 gaaaaagctt ttttcagtat aaaggctagt actaaagaag aggtcataat ttcactttca
    33181 aataaactaa aagaaagaga ttttatagag gaaagttcta taaaaagtat aatagataga
    33241 gaaaatattt catcaactga aataggaaat ctagtagcaa taccccacac aatagtgaag
    33301 ggagataaaa aatctataat aggagttgga atacttgaaa atccaataat ttgggataag
    33361 caagaggttc agctagtatt tatggtattt tttaatacta aagaaaaaca taatttctcg
    33421 atatttaaat acctttataa ttttataaaa gatgaaggcg gagtaagagg aattattaaa
    33481 atatgtgact ttaataaatt aatggcactg ataggtaatt aaggaggaaa ttaataatgg
    33541 gaacaaacat atttaacaaa gaatatgtat ttttaaatgt tgatgctaag tcaaaggttg
    33601 aagttttaaa atttatatca aaaaaagcta aagacttaaa cttggcagaa gatgagagct
    33661 tagtatatga aggtttaatg gctagagaag aacaatttac tacaaattta ggagaatcta
    33721 tagcaatacc tcacactaaa aatgatgcaa ttgaaaatcc agcagtagta gtattgaagt
    33781 ttaatgaaga tgttgtttgg aatgaaggtg aggataaggt taaattagca ataagcttac
    33841 ttatgccagg aaaaagtaaa gagaatattc atcttaaatt attatcatct ctttcaagaa
    33901 aattaataaa taaagaattt aaagattctc ttttaaaaag tgataatgtt gaagaaatat
    33961 ctaacttaat aaatgaagct ttaggtttat aattaaacac taaggttata aaataaaaaa
    34021 ataattaact aaaaatagga ggaatttgaa atgaataaaa aattagtagc attatgtgct
    34081 tgtccaatgg gactagctca cactttcatg gcagcagaag caatagagca agcagcaaag
    34141 gcattaggtt atgaagcaaa agttgaaact caaggtgctg atggtgtaca aaatgagtta
    34201 acaagagatg atatattagg agcaactatg ataatacatg cagtagcaat aacaccagaa
    34261 ggtatggaaa gatttgatgg ttgtgaagtt tatgaagttg aattacaaga agctataaaa
    34321 aatgcagaag gtgttataaa agaaatagaa gaagatttag gaatataata aatttattgt
    34381 ggaggaaata gaatggcaat taagaaaaaa gttataaaca gttcaggtgc ttctacaaca
    34441 ggaggaaata atgtaggaaa atctactcca tctagtccaa ctcaatcaaa gggaaatgga
    34501 aaatggaaag aaataagtaa acatataatg acaggtatat catatatgat accagtttta
    34561 gtaatgggtg gacttatagg ggctttatct caattaatac catatgctat attaggactt
    34621 gacccatctg taggtatagt tgatgctatg aactcaggag aatttacagg gttcaagctt
    34681 agtcttttaa atatagcaca attaatgtca aatttcggat ttactttgtt tggatttgcg
    34741 ataccactgt ttgcagcatt ttgtgcaaac tctataggtg gaaaaacagc tttaattgca
    34801 ggatttatag gtggatatat agctaataaa cctgtaggtg taccacaatt cgttgatgga
    34861 caatggactg aagttgttcc agttgcatca ggatttttag gtgcaatact aatagcattt
    34921 ataataggat attttgtaaa atggttaaat aaatctataa aagtttctca taactggtta
    34981 gcttttaaaa caacattttt aataccatta atagcgtcat tagcttgtat ggtattgatg
    35041 atatttataa taactccatt tggtggatta attaatgaaa gtatgaaaaa cttcttaaca
    35101 gcagcaggag cagctggaga atatgtatat gcaacagctt tggcagcagc tacagcattc
    35161 gacttaggag gaccaataaa taaagcagca ggattcgttg cacttggttt aactacagag
    35221 aatgtacttc caataacagc aagaacaata gctatagttg ttcctccaat tggattagga
    35281 ttaacaacat tattagataa aagattagta ggaagaagag tatatgatag acaattctat
    35341 caagcaggaa aaacttctat attcttatca tttatgggta tatctgaagg agcaatacca
    35401 tttgcacttg agagaccagg ttttgttata cctttaaaca tagttggaag tgtaatagga
    35461 gctattacag gtataattct aggagcaatt caatggttcc cagagtcagc tatatgggca
    35521 tggcctctag tagataactt atttggatat ataataggta tagcagttgg tgctatattc
    35581 atagcagtag gaaatatatt ctatagaaat aaattaataa aagatggtaa acttgttgta
    35641 gattatatcg actagtattt aatttttact atctataaat aactttggct ggtaatatgg
    35701 ttttaatcaa tattaccagc cttacctata tgattaatta aaggcggtgg agataagata
    35761 tggataagat agaaaaagta agagagtatt ttagagaata tgatatagat ggatttctta
    35821 taaatagtcc tacaaacaag ttttatgttg gaaatttatt tagctcttca ggttatgttt
    35881 ttataacgaa agaatcacaa tatataatag tagattttag atattttgaa gaaattaaaa
    35941 gaaaatctag tttgtttaat gtagtattaa tggacaaaac tagaactcat tttgatataa
    36001 ttaatgatat ttgtagagag cagaatataa aggaaattgg atttgaggga aatgaagtat
    36061 cttttgattt atatagaagt atgagtaata agttaagtgc aaccttaaaa tcagttgatt
    36121 tatctacatt aagggaaaca aaaaatgaag acgaaataaa atatataaaa aaagcttgtg
    36181 aaatagttga tgctacattt tatcatatag tagactttat aaaggttggt atgactgaaa
    36241 agcaagttga aaatgaaatt gttagaatta taaaagattt aggtggtcaa aaagaatctt
    36301 tcgatacaat agttgcatct gggcttagag gagcattacc tcatggaaaa gcaagtgaaa
    36361 aagttataga gtatggagat tttgttacct ttgactttgg tgctaagtat aataattatt
    36421 gttcagatat taccagaacc atttgtatgg gtactataaa taaagagtta gaagaaatat
    36481 ataatatagt aagaaaagct aatgaagagt gtataagggt tttaagaccg ggaatgacta
    36541 ctggagagat agataaagtt gcaagagaca taattggttc atatggatat gccaataatt
    36601 ttggtcataa tctgggtcat ggtgtaggaa ttatggttca tgaatatcct gcattagctc
    36661 ctgaatcaaa tgaagtatta aaagaaggaa tgattgtaac tatagagcct ggtatatatg
    36721 ttccaagtct tggaggcgta agaatagaag atgatgtact tataacacaa gatggatgta
    36781 tgagattgac tacatctact aaggatttaa tagttgtaag ctaaaaataa attagataaa
    36841 tattttataa caataagaaa agaagaggta aatttaaatg tataattttg atgataaacc
    36901 agatagagta agtgaaaagt gtagaaaatg ggatttaaat attataagag ataaatttgg
    36961 agatattaga gaagatttta taccaatgtg gattgctgat atggatttta aaataccaac
    37021 agaaatagaa aacaaattta ttgaagctgt aagaagaggt gtatttggat atacatactg
    37081 ttatgatgaa ttttatgatt cagttattgg ttggcaaaaa aatatgcatg aggtaaatgt
    37141 agaaaaagaa tggattacat taacttatgg aactgtatca acacttcatt atgtagtaca
    37201 agctttttgt aagaaaggtg atagtataat attaaacaca ccagtttatg acccctttga
    37261 atcatcagca aaaaaacaag gtgtaaatgt aatttgtaat acattagatg ttgtaaatga
    37321 tagatattat ataaattttg ataaattaga agttcaaatt aaagaaaata agcctaaatt
    37381 aatgatgttt tgtacaccgc ataatccatc tggaaggata tggacaattg aagaaatgac
    37441 aagagtagca actatatgta aagaaaataa tgttattttg gttgcagatg aagtacatgc
    37501 agagcatatt cattatggta aatttaattc aatacttaag attggaaaag agcttttaga
    37561 aaatgttatt ctattgacat caccaaataa gggctttaat cttggtggat taaagacttc
    37621 ttattcaata gttataaata aggatataag ggacaaattt agaaataaat taaagcaaaa
    37681 ttctataact tctccaaatg tgtttggaat aataggattg ataaccgctt ataatgaatg
    37741 tcatgagtgg ttaagtggtg taaatgaata tataaaatca aattatgaac ttttagaaac
    37801 ttgggtaaat aaatataata agataaaatt gatgaaaatg gaatcatctt acttagcttg
    37861 gatggatata agtggattag gtattagtgc aagtgagttt acagataaat tggctataga
    37921 tacaggagta ttattagaag atgggagtca ttttgtaaaa gatggtgaga agtatgttag
    37981 aataaattta ggaactcaaa aggaaaatgt aattgaagct ttaaatagaa tggatttatt
    38041 tttaaagtct ttataaaaat ttggttgaat tagtatttta tataaattta aactattaat
    38101 atggtatata cataagtaga atttgtttta gattacttaa gtatatacca tattttatat
    38161 aaaaattaaa tttgcattag tcatgtgata actatttaaa ttcatatgta ttttgtatgt
    38221 ataatagata taattattat ttaaacttat tgtttattta atttttcaat agaaaaagat
    38281 ttatctgtaa gtttaaaatt atcttttata cccttagtta ggtatacttt cttatcctta
    38341 gttatgaata tagcatctac attatcaatt ttattaatta aatccatacc tttttcaaga
    38401 ccaagtccaa atgtagaagt ggataaggca tcacaattaa tagatttatc agagattatt
    38461 gtaacactac ttaaattgtt ttcaaaagga taccctgtaa atggatttaa catatgatga
    38521 tatatttttc cgtctttttc gataaatctt tcataaattc cagaagtaac aacagattta
    38581 tttgtagttt ctatgtttcc tatggattca ccatttttac tagtggggtc ttgaatacct
    38641 attttaaaag gtttatcatt tttaccattt tctaaagtaa atatatttcc tcctaagttt
    38701 actaaacctt tttttacgtc ctctgatttt aaataggcta caattttatc agcagcatat
    38761 cctttagcta tggcacctaa atcaattttc atattttctt tggttaattt tatagattta
    38821 tttttttcat ccaatatgat gttcttataa tcaataagag gcagaagttt ttgtatctcg
    38881 gaatcacttg gaactttagc tttatctgtt ccaatagccc ataaatttga taatggacca
    38941 attgttatgt caaaatatcc atctgataat ttggaatatt gtatagcttc ttttacgacg
    39001 aaaaatgtat catcagaaac tctgacaaat tttttaccag cattatcatt tattttagaa
    39061 acatcacttc ctggtatatg agtgctcatt ttgttgtcaa tatatcttaa aattgaatca
    39121 cattcattta agattttatc acttttggat tcttttacat taaatacagt aacttcgttt
    39181 actgtaccaa ggtagtagct agttttacta tatttagtat tctggcttga actcctaaaa
    39241 aatgaaaata aaattaatgc taaaatcaat atcacaaaaa taaatgtgat ttttttctta
    39301 ttcatggtat attcctccta tgaaattgtg ttattatttt attataacat aatttcatag
    39361 gaggacaaaa aatgaagaaa aaagacattc ttcttatttt aggcattttg gttgttatta
    39421 cagcatgtta tgggataata aatgttataa attctaaaaa tgctggaaat attgagattt
    39481 atgtagataa taaattgtat aaaactgttt caataaatgc caaagaagaa tttaaaatag
    39541 agaatagagg ggggtataac atagtaaaga ttcatgataa aggggttgag atagtagatg
    39601 cttcatgccc tgataaggtt tgcgtacata ctggatttat aaacaagcca agtcaaagca
    39661 tagtttgtat acctaataga gtgagcataa aaataaatac aaatgaaaag aatgataatc
    39721 aagaagatat aatttctaaa taaaataata aaaataattg aaaggaattg gtgataatta
    39781 tggttaactt aggaaacgat tgggatgaat tattgaaaga agaatttgaa aaagattact
    39841 acttaaactt aagaaaattt cttatagaag aatataaaac acgccaaatt ttcccaaata
    39901 tgcacaatat atatgaagct ttaaaacaca cttcatacaa agacacaaaa gtcttaatat
    39961 taggacaaga cccttatcat ggagataatc aagctcatgg tttagctttt tcagttcaac
    40021 cacaagtgaa aactccacca tctttattaa atatgtataa agaattaaaa gatgatttag
    40081 gttgttttat acctaataat ggatatttaa tgccttgggc agaccaagga gtgttacttt
    40141 taaatactgc tcttactgtt agagcgcatg aagctaattc tcataagaac aaaggatggg
    40201 aaattttcac tgatagagta atatctattt taagtgagcg tgaagaccca gttatttttg
    40261 tactatgggg aagtaatgca cgtaaaaaag tagagttgat tgatacaagt aaacattata
    40321 ttttggaagc acctcatcca agcccattgt cagcaagtaa aggctttttt ggatgtaaac
    40381 acttctctaa gataaatgaa attttgaaaa agctaggaaa agaacctatt aattggcaga
    40441 tagaaaatat ataaaagtaa aaggtaacaa aataataaca aaacaacaat aaacaatgac
    40501 attgttgtaa ctaatagaaa tatgcttgaa tggttgaaaa tactatgtat gcatatttct
    40561 atttttttgt caagttgaca actcttgaaa aaatagttac aatttagtta cacaaaaaac
    40621 acaaataaaa aaagaaactt acaaagcaga attttatgaa gcaaaatata tagtgtaaaa
    40681 aatcttaggg aggaaatttt atatgaattt taaaaaattg agtaatgcta aagtctcggt
    40741 aatggcagtt gttttatcag ttggaatatt atcgggatac tctgcgctta ataaacaagt
    40801 aacattagta gtaaggggag aagaaagaac tatatcaaca tttagttcta atgtaggaga
    40861 ccttttagaa tctcaaaaca ttagctatga tgcaaatgat atagtaactt tagatgaaaa
    40921 tacaaagtta tctaatggag ataaaataga agtaattgat gtaaaggaaa aaacagttgt
    40981 agaaactaaa gacattccat ttgaagttac agttgttgaa gatggctctt tattaaaggg
    41041 tgattctaag gttaaagaag aaggaaagtc tggtaaaagt gaattaatat ataaagaaac
    41101 ttatcacaat ggaaagaaag tagaaaagga atttgtgaaa aaggttgtta aagctgagcc
    41161 agtaaataag gttgtaagca aagggacaaa ggtagaaatg caagtagcct catcaagggg
    41221 agaaagttca agaagaatgg cttcatcatc atctagtagc agtagtagta acaatggtaa
    41281 aaatatgaga gtggtagcaa ctgcatatac tggtcacagt ataactgcaa caggaacaaa
    41341 acctaagtgg ggaacaatag ctgtagaccc taaggttata ccatatggaa ctaaagttta
    41401 tatacctcaa tttgacatgg tgtttactgc agaagactgt ggtggagcaa taaaaggaaa
    41461 taaaattgat atctacatga atgattcaaa aacagtttat aactggggaa gaaaaactat
    41521 agatatacgt atattaaaat agttaaataa tttatatttt tactggaaat tatattttga
    41581 ttatatagag aagaaactct ttatagattc taaaaatata atttccagtt ttttattttg
    41641 gatatgtaat aaaacaggta aattttaacc atgtatagat aaaagctatt taaattataa
    41701 tttactatca tataaatcta taaaaattgt tgcaaagagt caaaatatat gatattatag
    41761 atttcgttga gaaggatttc attatgggag tggatgggtg ccggtgtgcc ctctagtctt
    41821 caaaactagt atgaggggtt aagagcctct taggtgggtt cgattcccac gcattcccgc
    41881 caatatgtaa aaggtatctt ttaattaaaa gatacctttt taaatttatt ttaagataat
    41941 ttatttttaa taatatataa tgttttgtat tatataatgt aaattttcta atactattat
    42001 atatttagat aaaaatctag gagtgtggaa agatggaaaa taattttaca acgagaacta
    42061 gttttctagt tggagatgat gggataaaaa aacttaataa ttcaaatata atagtatttg
    42121 gagttggagg agttggtagt tttactgttg aagctttagc aagagctggg gtaggcaata
    42181 ttactattgt agattttgat gatgtagata taaccaatat aaatagacaa ataccagccc
    42241 tacattcaac tgtaggaaga tacaaagttg atgtcatgga agaaagaata ttagatataa
    42301 atcctaatat caatataaaa aaaataagaa gtctttataa taaagatact agtgacgaaa
    42361 tactaactga aagatatgat tatgttgtgg atgcgataga catggtaagt tccaaaatac
    42421 atctaattga aacttgtgaa aaaaaaggat tgaaaataat aagttctatg ggtatgggaa
    42481 ataaattaga cccaactaaa atagtcgtta cagatataca caaaacaagt acttgtcctc
    42541 tagctaaagt tatgaggaaa gaacttaggg atagaggtat caaaaagtta aaagtagtat
    42601 attctactga acaaccaata gaacttaaga agaaagtaat gaatggtaga aaagtaacac
    42661 ctggtagtgt atcatttgtc ccatctgtag gaggtttaat aatcgcatct gtaatagtaa
    42721 atgaactatt aggataataa gttttaaata ttttaattta taaattttat atacaaattt
    42781 gaaggctaga agcatgaata taaagcaatt ttatattcat acttttagcc tagttatttt
    42841 atattacctt attttatgta gttgtagttt tattttttag attatgtatc tatttcataa
    42901 ttatattttg taaatataat ttctccatca ctataaaaaa caatatctcc tattctgtct
    42961 gttctataaa ttgatacatg gttttgtttt agattattaa taacctccct atgtggatga
    43021 ccatattgat ttttatatcc acatgatatt gcagctatat ctggactaat tttttttata
    43081 aattctaaag atgaagaaga attacttcca tgatgaccta tttttataaa attaatatta
    43141 tttaatttaa aagaatgtaa aatttctttt tcattttctt cttcagcatc acccataaat
    43201 aaaaaagaat tatcattaaa agttaatttg aaaactatag aatttaaatt agattcttct
    43261 tgtatatatg atggacttaa tacatatata tttatattgt tatctatgtt taaaacatca
    43321 tttttataga gatgctgaat agataagttt ttatccgtac aagaatttat tagattttga
    43381 taagcctcac tatcagttga ttgttcaggc atatatatgg agtttacatt aaattttttt
    43441 ataatattat caagacttcc tatgtggtct gaatctggat gagttgctat tataatatca
    43501 atagttttta ttcttttttg tctaagataa ctaataatta tattttcgct atcttcatca
    43561 cctccatcaa ttaatatatt cttatttgtg ggtgtttgaa ctaaaatact atcaccttgt
    43621 ccaacatcaa tcatatgtat agatagtaga gattttttgt cacagccgat taaaagtgat
    43681 attataataa gtattgataa aaaaatttta tttctcaata aaaaatcact ccattcatta
    43741 catgtttata taaatttatg atgggtaata taagatatat atgacttatt atattttata
    43801 ttttaaacaa tataaatgaa attaatacca taataaaatt ggagtttttt atatatacaa
    43861 aataaaattt ttaaaattaa gcaataaaaa aggtaggagg attttatgtt tttcaaagat
    43921 attgaactaa attcaaaaaa agagttagac ccatattttg atttagtaga ttatgaagct
    43981 tgtgagtact gttttagtac tttatatatg tggcaacatg tttataagac aggatattat
    44041 ataggtgaag attttgccgt tttagttggg gaatacgaag gagatagctt ctcaatatta
    44101 ccacttgcta aaaaagataa acttccagaa gttgtagatt ttgtccttga atatttttct
    44161 aaaaataata aaaaaatata cttaagaggt attactactg aagtagtaga gtttttaaaa
    44221 gaaaaatatc caggtagatt tgagtatata gaagaaagag atttatttga ctatatttat
    44281 gatgcagaaa gtttaagaac tctagctggt aaaaagaatc aaaagaagag aaaccatata
    44341 aactatttct taaaagagta tgcaggtaga tatgaagcta aattattaga taaagaaaac
    44401 tttgatgagt gtttagtact aatgaaggaa tgggaaagta ataaggaaga aaataatgaa
    44461 tttgatgaaa gtatggatga tgaattaata ggtatcaaaa agatatttaa ccattatgac
    44521 atattaaaag ataaggtaaa agtgtttggt gtttatgtag atggaaaatt agaagctttt
    44581 agtataggag agcttttaaa tccaaatatg gcattgattc atatagaaaa agctaatccc
    44641 gatataagag gtctgtatcc atttataaat caacaatttt tggtaagtga atttaaggac
    44701 gttgagtttg taaatagaga agaagattta ggtattgaag gattaagaaa agctaaactt
    44761 tcttatcatc cttgcagatt tgttgaaaag tacagtgtta gggaggctta atagactata
    44821 tggagattag gtatgctaaa gaagaagaga tagaaagtat aaaagagatt tggagttatt
    44881 gttttaacga tacggagagt tttatgaagt actattttaa tgataaatat aaatctgaaa
    44941 atactgtagt agctttagat gaaggaaaaa ttatttcttc tcttcagcta aatcagtata
    45001 agttattact aaatagcaag gtatataata catcatatgt agtaggagtt tctacacttc
    45061 cagagggaag aggagctgga tatatgaaca aagtaatgaa atttacttta aacgaattgt
    45121 ataaaaaagg gcaattagtt tctatattga tgcctattga ctatagactt tatagaagat
    45181 ttggatatga gcattgttac gaccagattg aatatactat taacacagat gatttaaaaa
    45241 attttaagtc aagtggaaaa atgataaagt ctaatttatc acaaattgat gatttgatac
    45301 aaatagatag aactttttta aatgaggtta atggtaatgt gttaaaagat gaacattatt
    45361 atgaaaactt atttaaagag attcaaagtg aagatggatt tttatacatt catgaaggaa
    45421 atgaaaaaga tggatatata gtttattttc ttcaggaaga taaaatgttt gttagagagt
    45481 tattttataa aaatatagat gcccttaaat caatgcttaa atttatatat aatcacaata
    45541 ctcaatgtaa gatagttact atatctactc ctacaataga taagataaga tttatattag
    45601 acaaccctaa agatagtgat ataaaaataa aaccttttat gatgggaaga gttataaatg
    45661 taaagaagtt tattgaagat atagatattg agaatgatat taatagctca tttaatttat
    45721 taatagaaga caagtttata gatgaaaata atggtctatt taaaatttct atacagaata
    45781 agaaggtatc tgtag
//
LOCUS       DAGSHT010000011        45686 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGSHT010000011 DAGSHT010000000
VERSION     DAGSHT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.012"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(70..2142)
                     /locus_tag="MJB72_000563"
     CDS             complement(70..2142)
                     /locus_tag="MJB72_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY8261734.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(2445..3287)
                     /locus_tag="MJB72_000564"
     CDS             complement(2445..3287)
                     /locus_tag="MJB72_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8261735.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(3548..5590)
                     /locus_tag="MJB72_000565"
     CDS             complement(3548..5590)
                     /locus_tag="MJB72_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBY8261736.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(5742..6713)
                     /locus_tag="MJB72_000566"
     CDS             complement(5742..6713)
                     /locus_tag="MJB72_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBY8261737.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(6714..7691)
                     /locus_tag="MJB72_000567"
     CDS             complement(6714..7691)
                     /locus_tag="MJB72_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261738.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            7910..9469
                     /locus_tag="MJB72_000568"
     CDS             7910..9469
                     /locus_tag="MJB72_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8261739.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            9575..10534
                     /locus_tag="MJB72_000569"
     CDS             9575..10534
                     /locus_tag="MJB72_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8261740.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            10537..11349
                     /locus_tag="MJB72_000570"
     CDS             10537..11349
                     /locus_tag="MJB72_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8261741.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(11565..12467)
                     /locus_tag="MJB72_000571"
     CDS             complement(11565..12467)
                     /locus_tag="MJB72_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8261742.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            12822..14000
                     /locus_tag="MJB72_000572"
     CDS             12822..14000
                     /locus_tag="MJB72_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBY8261743.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDILIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            14009..14665
                     /locus_tag="MJB72_000573"
     CDS             14009..14665
                     /locus_tag="MJB72_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBY8261744.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            14665..15330
                     /locus_tag="MJB72_000574"
     CDS             14665..15330
                     /locus_tag="MJB72_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBY8261745.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            15435..16211
                     /locus_tag="MJB72_000575"
     CDS             15435..16211
                     /locus_tag="MJB72_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBY8261746.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQESLNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            16273..17607
                     /locus_tag="MJB72_000576"
     CDS             16273..17607
                     /locus_tag="MJB72_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY8261747.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            18104..19030
                     /locus_tag="MJB72_000577"
     CDS             18104..19030
                     /locus_tag="MJB72_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBY8261748.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(19423..22962)
                     /gene="nifJ"
                     /locus_tag="MJB72_000578"
     CDS             complement(19423..22962)
                     /gene="nifJ"
                     /locus_tag="MJB72_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBY8261749.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(23480..24049)
                     /locus_tag="MJB72_000579"
     CDS             complement(23480..24049)
                     /locus_tag="MJB72_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBY8261750.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(24121..24729)
                     /locus_tag="MJB72_000580"
     CDS             complement(24121..24729)
                     /locus_tag="MJB72_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8261751.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(24737..26500)
                     /locus_tag="MJB72_000581"
     CDS             complement(24737..26500)
                     /locus_tag="MJB72_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBY8261752.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(26543..27010)
                     /locus_tag="MJB72_000582"
     CDS             complement(26543..27010)
                     /locus_tag="MJB72_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261753.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            27193..27654
                     /locus_tag="MJB72_000583"
     CDS             27193..27654
                     /locus_tag="MJB72_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261754.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(27747..27917)
                     /locus_tag="MJB72_000584"
     CDS             complement(27747..27917)
                     /locus_tag="MJB72_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261755.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(28106..28327)
                     /locus_tag="MJB72_000585"
     CDS             complement(28106..28327)
                     /locus_tag="MJB72_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBY8261756.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(28411..29637)
                     /locus_tag="MJB72_000586"
     CDS             complement(28411..29637)
                     /locus_tag="MJB72_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY8261757.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKLALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(29699..30631)
                     /locus_tag="MJB72_000587"
     CDS             complement(29699..30631)
                     /locus_tag="MJB72_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBY8261758.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVIF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(30720..31247)
                     /gene="hpt"
                     /locus_tag="MJB72_000588"
     CDS             complement(30720..31247)
                     /gene="hpt"
                     /locus_tag="MJB72_000588"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBY8261759.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            31476..32552
                     /locus_tag="MJB72_000589"
     CDS             31476..32552
                     /locus_tag="MJB72_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY8261760.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMLSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(32638..33846)
                     /locus_tag="MJB72_000590"
     CDS             complement(32638..33846)
                     /locus_tag="MJB72_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBY8261761.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(34007..34396)
                     /locus_tag="MJB72_000591"
     CDS             complement(34007..34396)
                     /locus_tag="MJB72_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBY8261762.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(34500..35501)
                     /locus_tag="MJB72_000592"
     CDS             complement(34500..35501)
                     /locus_tag="MJB72_000592"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBY8261763.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(35560..35815)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35949..37139)
                     /locus_tag="MJB72_000593"
     CDS             complement(35949..37139)
                     /locus_tag="MJB72_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY8261764.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCLEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(37420..38541)
                     /locus_tag="MJB72_000594"
     CDS             complement(37420..38541)
                     /locus_tag="MJB72_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBY8261765.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(38602..39087)
                     /locus_tag="MJB72_000595"
     CDS             complement(38602..39087)
                     /locus_tag="MJB72_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBY8261766.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(39089..39757)
                     /locus_tag="MJB72_000596"
     CDS             complement(39089..39757)
                     /locus_tag="MJB72_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261767.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            40147..42171
                     /locus_tag="MJB72_000597"
     CDS             40147..42171
                     /locus_tag="MJB72_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261768.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(42401..43588)
                     /locus_tag="MJB72_000598"
     CDS             complement(42401..43588)
                     /locus_tag="MJB72_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBY8261769.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKSKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(43790..45061)
                     /gene="brnQ"
                     /locus_tag="MJB72_000599"
     CDS             complement(43790..45061)
                     /gene="brnQ"
                     /locus_tag="MJB72_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY8261770.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(45202..>45686)
                     /locus_tag="MJB72_000600"
     CDS             complement(45202..>45686)
                     /locus_tag="MJB72_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY8261771.1"
                     /translation="CIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFK
                     NSKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLV
                     FEYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK
                     "
BASE COUNT        15697 a         7566 c         5560 g        16863 t
ORIGIN      
        1 tgcatgttaa ctgaacctat ttatatattt attaaatctc acataacttt tagttacact
       61 atcatatagc tatcctattt tttcaatatc atctcttaat atacttactc tattctcctc
      121 ttttgctgtg gttactcctt ttttaatgct taatttctga ttttgttcta aattcgtaaa
      181 tactacagga cttattgttg atagaccatt tttttcaata tattctaagt ccattttaac
      241 taatttttct cccgctttta cttcatctcc tacattcaca aatacttcaa atccatttcc
      301 ttctaagttt acagtttcta tccctaaatg tataagtatt tctactccac ttttttggga
      361 ttttattatt attgcatgct tagttggaaa tataactcca atagttccat caactggtga
      421 ataaatcaat ccatcactag atttaattgc aaatccatcc cccatcagtt tacttgaaaa
      481 tacagaatct ggcacttctt ctatatctaa tatctctcca tttgttaatg aatcaatctt
      541 ttctactaaa gcattttcca tatttgcaac ttccatattt tttttcactt tatttttatc
      601 aacattttta gtaacagtac tttcaacctt tgtttttccc tctattatat cttttatatc
      661 agactttatt gaatccgatt ttggtccaaa tatagcttga acattgttgc caataaccat
      721 aacacctgct gctccaagtg atttcaattt ttctttatct actttattta tatcatttac
      781 aaccaatcta agtcttgtta tacacgcatc taggctcttt aaattgtctt tatttcctaa
      841 agcaatcaaa atctctgatg ctaattcatt cttagacatt tcaatgactt cagcgttagt
      901 ttctgactca tcttctcttc ctggtgtctt taaattaaac tttcttatca taaatctaaa
      961 tacactatag tataatactg caaaaccaat acctaccaag atagccaagt accatggagt
     1021 tctattagga agaacaccaa ataataagaa atctattaat cctcctgaaa atgtgaatcc
     1081 aattttaact cctaataatt gcataattgc gtatgataaa cctgaaaata aacaatgtat
     1141 tgcaaataat actggtgcaa caaataaaaa cgcaaactct attggctcgg ttattcctgt
     1201 taaaaaagag gttaatgctg ctgatgccat tatacctgct acaaattttt tcttttctgg
     1261 ctttgcttca tgcaccatag caagagctgc tgctggaaga ccaaacatca taaatggaaa
     1321 tgcacctgtc ataaaagtac ctgctgtgaa atttactcca tctttaagtt gagcaaagaa
     1381 tatcttttgg tcacccatta caagctcacc agctttattt acatattctc caaattgata
     1441 ccaaaatgga ttatggaaaa catgatgcaa accaaatggt attaatagtc tttccaaaga
     1501 accttccata aatgctgcta gtgtttgatt tgcttctata acattttttg agaaactaag
     1561 taatccctct tgtattggag gccaaataaa agacaatata attcctattg ctatagcaac
     1621 taatgaagta acgattggta caaatctttt acctgcaaaa aaacctagat actgaggtaa
     1681 ttcaatttta taaaactttt tataaagata agcagccact atccctataa taataccacc
     1741 aaatacccct gtttgaattg ttggtattcc aacaatattt gcatacatag aattgtctac
     1801 catatcttct gtaacacctg ccataacacc catagatgta ttgagaatta aaaaacctac
     1861 tatggctgcc aatgctgcaa caccttctcc ttcagttaaa ccaacagcta ccccaactgc
     1921 aaatataagt ggtaagttat taaatataat ttgacctgaa ctttccatta tacttattat
     1981 aagttgaaat gtaggattcg ccaaagctgg aaaatgagcc aacaatgcgt ctcctgctaa
     2041 agcattactc acgccaagta atatacctgc tgcaggaagt aatgcaactg gtagcattag
     2101 agattttcct actttttgta aaacaccaaa tacttttttc atttactttt ctctccttat
     2161 tattttatat ttttttaaat aaaaaagact taagtttata aaaagttaca ttagtatact
     2221 tagttctaaa attaaactta tgttaatata aataaaccta tattaaccta atttcagaat
     2281 taaaaataaa ctattataat ttttttataa acataagtct tgcctgcatt atcagtaaca
     2341 atcttaatgt tttaaaatta ttattttata ttaaatttta aataatatat taaaattaaa
     2401 cataatatat taaatctata cattctgtat taaactactc tttattataa attcagtaca
     2461 gatataactc ttttgatatg taatgttaaa taaagtagtt catcttgact tacatagtaa
     2521 tcataattct tttctacatg agtttttatt ttatatgcac aatcataatc cttttcatac
     2581 tttactttaa ttatatctat aatttcatta tttgtatcat tgattttttc tttttttact
     2641 aatcgttttg caaagaattt taaatgagtc agaagcctat cataatttat atcatcttct
     2701 ttaaattcaa ctctataata atatcttata atatttagta tgtcttttac aattttagta
     2761 attaatagtg attcctttga acttccctta tagtttgaat ttactatatg cattgctata
     2821 aaaccagctt catctactgg aaatttcaca tcaaattctt tatttatata atctacagcc
     2881 catttcccaa tctcatattc ttttttatgt attcttttta tctcatgtag taaatcattc
     2941 tttatagtta tgttttcatg atatctttta attgcaaatg caatatggtc agtaagtgct
     3001 acataaatat attcataaag cttagtattc aaattctcct ctgcataagc aattatttca
     3061 gtactaacct caaaaaattt ctcatcaacc tggcttgcta gtttactaat tttatctaaa
     3121 aagctttcat cttgtattac aaaagttctt tcaattttac ttttatctat ttcttgacct
     3181 ttcttttttt gaaacccaat tccacatcct gttaatatta attctttttt tatattttca
     3241 tctaaaacta atacaacatt attattataa atttgttgaa taatcataat caatatctct
     3301 cccaaaatac aatttccctt attcataaaa aaatacctaa attaagatac aattcacctg
     3361 tataccttaa cctaggtatt gcctgcttac cagtaacaat ccagcttgtt aacgcttata
     3421 tattgtattc aatttatggt tttattctat tataaataaa tatatatgtc aagtaaatta
     3481 ttgaaaacta tttcctattt tttagtatta attttaacat caaaaatcaa attttattcc
     3541 attatattta tgattctatc atcatcattt catcatagag aatattttcc aataactctt
     3601 tagccacttc tcttgatatt tgcttagttt caactaattg ttgaacactg tctctttgac
     3661 tttgtatcgc cattactctg tagtattcta ctcttgcctc aaacttatca tcattttgaa
     3721 tctttctagt aatccaattc aaataacgat tatagaatct gattgctatt aaggcctcat
     3781 gtctgttatc ttcatcagta tttttattta taaatgatat agcagtttta gtagaaagtg
     3841 catatgcatg gtgaaactct ttgattattt ctttattctc agatactatt tttgtaattt
     3901 tttcttttac ttctttcttt acttttctat ttataatcca catgcttatt tttaatttta
     3961 caattttaaa tatagatttt tgaacagatt ttcttgattt tgtaatataa aatctataca
     4021 atttaaaggc tctatcagaa attttaccat cttctaatag tttcaaaaca gctaataatc
     4081 cctgttggtc tgcttctgca atcatcatac ttataagttt attatctgga gtatttagta
     4141 ttcctttttc tgagtctacc aactgttcat ttaaatcatg tataactggt tgtacttctt
     4201 gtgctttttc attactaagt tttaaaatgg cttctttcaa aattagttta tatgcctctt
     4261 tatcagataa taaaggttct gactcttgta cgttattttt taataatctt ggcagtaaaa
     4321 ctgttgccaa tactaaactt ataagaataa cttctgatga tataaataaa acagtatctc
     4381 tcattggaaa tacatcttta tcagccaaat aaaaaggtat agataaggca gttgctagtg
     4441 taaatgtacc atgaacacca caagtagcaa ctattagtga gtatttacat ttagaaatac
     4501 tctcttttac aacttcttca tccttaaaac ctaaaaaacc tacaaaaaaa ttatttaaag
     4561 gattcttttt aggttttata aaagaattat gccataaata tacccatata aatcttataa
     4621 caaataaaat aagtacaatt aaagtagaaa taaaagtcaa ttccaatgcc atattttcat
     4681 ttttactact aagtcctgta tatatacttg gtaacaaaaa accaagcagt gtaaagacaa
     4741 atccattcaa tacatactcc aaaacatacc aagtattgtc tgatattatc cttaatttag
     4801 tagttgtatt ttgtaaatgc tctttttcaa ttccatgagc tatccctgcc attacaacag
     4861 ctaggattcc agatactcct atttcttctg ctacaaaata tacaaaaagt ggagtcatta
     4921 tttgtataac tattaccatt ggaatttctt ccaaattcca cttgtgtaaa ctaagtctta
     4981 tttttataat tatgtatcct aaaatacttc ctactattat tcctccaaaa gctgtaatca
     5041 taaattgaat ccctgcctct tctattgaaa aaactcctgt aactgtggca agtactgcaa
     5101 ccttaaatgc tactactcca gcagcatcat ttaataaaga ttcaccttca agtatactca
     5161 tcatattttt aggaaaattt aatccttgtg taattgattt tactgctact aaatcagttg
     5221 gagatattac tgctgccaaa gcaaatgaaa cagataatgg catcccagga ataagaaaat
     5281 gtataaagat tccacctgca aacacggtta ttaaaactaa accaaatgca agaagcaaaa
     5341 ttggttttct gagctctaat agctcttttc tcgatacatt ttgtccttca ctgaatagta
     5401 atggagctat gatacatatt acaaatattt ctggattaaa tgaaaatgag aatttaaatg
     5461 gcaaaataga caatataatc cctagtacaa tttgatatat tgcaagagga atattactcc
     5521 actttttgct aaatacagtt gaaatcgttg tacaaaccaa taatcccaac aatgttacta
     5581 aaattgacat aactacctct cttttcaata ataatattat agattaaact tatatatcaa
     5641 ctaacttaat attaacagca tgaaatccca tcttaatcaa aaaaattttc agtttattct
     5701 attattctta ttatatacat caaatttaat ttttgaaata tctatactag atgacatgca
     5761 acaaaatgat ttcttgatat ttcttttaaa actggaatct cttttttgca tatttcctta
     5821 caataagggc atcttgtatg aaacttacat ccacttggtg gatttgttgg agatggtatg
     5881 tctcctttta atataattct atttttctta attgtagggt cttgtattgg tactgcgctc
     5941 ataagcgctt ttgtatatgg atgttgagaa tgtaagtaaa gttctccttt agttgcaatt
     6001 tctattatag aacctagata cataacacaa actctatgtg caatatgatt tactacactc
     6061 aaatcatgag atataaataa atatgaaagt ttatatttat cctgtaaatc cataagtaaa
     6121 tttataattt gagattgaat agaaacatct agtgcagaaa caggttcatc acaaactata
     6181 aattctggat ttaatgctaa tgctcttgct atacctattc tctgtctttg acctccagaa
     6241 aactcacctg gttttctatt taaatggtac ttagaaagac cacaactttc tacaacattt
     6301 aatatattat cttttatttc attctttggc actaaattat gttctataac tgcctcggat
     6361 attatttgtt ccacatttaa tcttggattt aatgaactat atgggtcttg aaatatcatt
     6421 tgcattttgg gtcttaactt tctaagttct tccttctcta acttatatat atctacacca
     6481 ttaaattcaa ctttaccttc agttttttca attaatctaa gaatggttct tcctgcagtt
     6541 gattttccac atcccgattc tccaactata gccaaagttt ctcctttttt taaatcaaaa
     6601 gaaattccat ctactgcctt tacacattta atttccttat caaataatcc agatttaact
     6661 tcaaagtact ttttaagacc ttctactttt aataaactct cttctttttt caattatatc
     6721 tcctcctttt tatataacca acatgcaata ctatgaccac tattcaattc tattaaagga
     6781 ggtatctgag ttctacattt atcacatact ttttcacatc tatcactaaa ataacatgaa
     6841 tctggcatcc caattggatt tggtacttgt cctggaatcg aatacaatct tttatctttg
     6901 ttttttccaa gagcaggctt tgattttaga agtccaatag tataaggatg ttttggattt
     6961 ttaaataatt caacaacctc tgccacttct ataattttac cagcatacat cactaatact
     7021 ttgtcacaca tttctgcaac tactcccaaa tcatgtgtaa tcatcattat agatgtgttt
     7081 aatttttctt ttatattttt cattatatct aaaatctgag cttgaatagt aacatctaaa
     7141 gctgttgttg gttcatcagc tataagtaat tttggattac atgataaagc catagctatc
     7201 attattcgtt gtctcatacc acctgataat tcatgtggat atgaattata tatttcttca
     7261 gctcttggta tttctaccag ttttatcata tcgattacct ttttcttagc ttcttccttt
     7321 gataaatctt gatgtatcat aataccttca atgatttgac ttccaacagt aaacgctggg
     7381 tttaaagatg tcattggttc ttgaaaaatc attgatatct cattccctct tattcccata
     7441 agttcatctt cattcatctt taaaatgtct ttgcctcgaa agattatttc tccaccttct
     7501 atttttgatg ttggaagtaa cttcatcaaa gacatggctg ttacagactt accacatcct
     7561 gattctccaa ctattccaat agtttcacct tcatttatat taaaacttac ttcatttaca
     7621 ctctttacaa tccctttatc tgtatgaaaa taaactttta aatctttaac ctcaattagt
     7681 gctttactca ttattttctc ctctcagcaa aattacttaa gataacatac accttttgat
     7741 tgtattttat aaaaatttaa attacaacat tttcttacat tttattattt ttttctatta
     7801 attataataa tataatagtt aatttctata tatattgatt taataatgat tactattata
     7861 caataaaaaa tatgtgttaa atattatttt atggaggttt tataagaata tgaaatttaa
     7921 aaaattggca tcattaatct tagtttcatc attaatgctt acatttacag cctgtgcttc
     7981 caatgataaa gacaagtcta gtggaagcaa atctggtgga actgttgtaa tacctatggc
     8041 aagtgaccca gatataatca atggagcttt tgcaaacgtt aaagaagact ctcttgcatc
     8101 aaatatagtt tatgctcctc tttatactta tgaaaaagga aatttagtaa attatttagc
     8161 agaaaaagta gattttaaag atagtaagga attaactata aaattaaaat ccaatttaaa
     8221 atggcatgat ggaaagccaa taactgctga agatgtctta tttacattta atacagtttt
     8281 agacgaaaaa caaaattcac catcaagaca atacttattg gtgggtgaga aaccagttaa
     8341 agtagaaaaa attgatgatt taacagttaa gattacttta ccaactgcaa gtgaatcttt
     8401 cttatatggt atatctaaaa tttctccaat accaaaacat gtgtttgaag gagaatcaaa
     8461 tatagctaag tcagaaaaaa ataataatcc agttggttct ggtgcattca agtttaagga
     8521 atggaaaaaa ggtgaaagca tagtatttga aaagaatgca gattattttg gtggagaacc
     8581 taaggcagat tctatagcgc taaaaataat tcctaatgaa gcatctcaag aagcagcttt
     8641 aaataatggc gaaatttcac ttatgaagac atctgctgaa ggatatgaaa aggcaaaatc
     8701 aaactcaaac ttacaaactt atacttatag tgaagaaaga ttaaactaca ttgtatttaa
     8761 ccaaaatata tcaaacatgg ctaacaaaga agtaagacaa gccttatcat atgccttaaa
     8821 tagaaatgaa atgatagaat cagcatatgg aaaagaaggt tctgtacctg ctaaatctat
     8881 attagttcca gaagcagact tctatacaga agaaggtgtt gaaggatatg accaagatac
     8941 aaataaggct aaagatttat tggataaaag tggtgttaaa attgataaat taaaaatagg
     9001 ttataacaca ggtagatttg ggcataaaaa ctatgcctta gtagctcaac aagagttgaa
     9061 aaaaattggt atagaagctg aaatagttcc ttatgaaagt aaggcattct tcaatatact
     9121 atttagtaat agcactgaat gtgatatgta tgtaaatggt tatgcttggg gattagaacc
     9181 aaacccatat agaggtatgt ttgaaactgg tcaatattgc aaccaaacta aatattctaa
     9241 tgctgaaata gatgctttat gggaaaaagg atttactgag ttaaacaagg aaaaacgtga
     9301 agaaatatat aagcaaatac aacaggatat atctaaggat gcgcctattt acactataga
     9361 ttatgaacaa aatttaatgg cagctcaaaa aaatctaaaa ggaataaaag atgctaaacc
     9421 atctcctgca atattatttg aagattggtc taaactgtat gtagaataat tgatgtaaaa
     9481 ttttatatta ttaattagaa aaggagatgt ctaaaaataa tattgtttta ttattttaaa
     9541 acgagacatc tcttttaatt aggagaattt acttatgtta aaattaatat tgaatagagt
     9601 aaaagtttta atacctatgt taatcttaat atctatatta tcatttggac ttttagaatt
     9661 agctcctggt gacccagcag atgcatatat aaatccttta atgtcttcac aggatattga
     9721 aaatataagg gttaatatgg ggcttgataa accagtatat ataagatatt taaactggtt
     9781 gaaaaacact ttaagtggaa acttaggtat ttcttatata aaccatatgc ccgttacaga
     9841 acaaattatg gaaaaaatgg gaaatacatt tattcttatg ggtacttctt tgatattttc
     9901 aatattggta gctatacctc ttggaatatt tttagctgta aataaaaatt caataactag
     9961 taaagtatca tctgttttta attatatagg tgtttctata ccttcttttt ggataggtat
    10021 gatactcatt tcaatttttt cagttaaact taacattttc ccatcaggag gtatgcatac
    10081 tattggaaat gatagtattg gagatttagt aaaacattta gttttgcctg taataacttt
    10141 aggtctatat aatactgcta tttttacaaa ttatgtagaa gctagtgtaa atgaacaatt
    10201 aaaaaaacaa tatgttatta cagcaagagc aaaaggatta tcagaaaaag tcatactatt
    10261 taaacatgtc ttgaaaaatt cactgacatc attggttaca atcctaggca tgagtataca
    10321 aaaattagtc actggtgcat ttgttacaga agtagttttc tcttggccag gtatgggaag
    10381 attaatgatt gattcaattt tttcaagaga ttatactgta attatggcaa taactatgtt
    10441 atcagcacta tttttaattt taggtaattt agttgctgat atactatact tattaattga
    10501 ccctaaaata aaatctagca aaggaggatt ctagttgtgt ttacaagtat cttaaaaaaa
    10561 ttaaaaaaag ataaatcctt tgcttttgca tgtattactc ttagcataat aatcatatta
    10621 attataactt cttttatatt aaatatagac tctaatgcaa ttgatactac ttctaaactt
    10681 caagaaccta gcttaaagca tatctttggt acagacgaac ttggtagaga ttacttctct
    10741 agagctcttt atggtggaag gatttcactt agtgttggta ttttatcaat gcttatttct
    10801 gtagtatttg gtactgctgt aggtgttatt agtggatata ttggaggtaa gctagaccaa
    10861 ttcttgatga gattagtgga tatgcttatg agtataccaa gtttcttagt tttgattgta
    10921 ttaaatactt atctaacacc taaagtttcc actattatac taattattgg attcttaagt
    10981 tggatggaag tttcaagaat tgtacgtggc gaaacattaa aaataaagga aaatgaatac
    11041 tgtctagctg ctaaagctac tggtatcaaa actagaaata tactattaag acatatagtt
    11101 ttaaacttaa aagagactat tgtagttgct ggttcgttaa atattgctaa tgctatctta
    11161 actgaatctg ctcttagttt tttaggtctt ggaatccaat tgcctcttgc atcatggggt
    11221 aatatgcttc aagatgctca aagacatatt tttgacaagt tatatttagc tgtatttcca
    11281 ggattactta tatttttaac agtcatgagt ttgactataa taagcaaaaa attttctagt
    11341 aatacttaac aaattttata tagaaaataa aaacagtgat tatctaaaga tatacactgt
    11401 ttttattttt cgtttaaaaa aatacatatt ttgtatatta taactttaaa tactactcta
    11461 ccatgttatg taaagcctat tattgctctc aaacatatag atgaaaaact aatagtttat
    11521 tataataaaa attgatttat ttatttgtta ttttaataac atttttaaga gttcttttta
    11581 ctaaagaaat ctataataaa tttaaataca tgttggtcta catggctata acgttctttt
    11641 ttaggtctac atagtataac tttccatgta ataggatgat taaaaggctt ttctataatt
    11701 ttgctatttt caaaaaaatc atgaactggt gatggtagaa ttgttataaa atcagaattt
    11761 gtggttgata gcaacaaaaa atcccatgaa ccagacatta tatatctttt taacttaacg
    11821 ttttcatcag taaatttttg cattagctta tgatgtatca taaaagtagg attaaatatt
    11881 gcaagtaact gctcattcaa atcgttccaa tctatcttat ccttcttagc aagtggatga
    11941 tttatattta aaaatgctgt aagttcatct tcttgaagca catgttcagt tactacactt
    12001 ttatcaattt tatgtggatg taacaatact gcataatcta attcctgtaa gataagcatt
    12061 ctatttaagt catatgcacc cttctctact atatcaaatt ctatatctgg attatttgca
    12121 actaactgtg caactacatc tgaaaatgct attccaagta taagtggagg tattcctatt
    12181 ctaacttttc ctttaaattg aactgaatct tctcttaaat cttccatcat atttttgtat
    12241 tcatttatga tatgttctgc atttacataa aatctttctc ctgctggtgt taacccattt
    12301 aacctgcctt tatacctttc aaataaatat acatcttcac tttcttcaaa attttttata
    12361 atttgactta atgctggttg agatatgtga agtgtcttag atgctattga taaattaaaa
    12421 ccagatttca ctatttcaac aaaatatttc aactgtttta tatccatttt aaaccccctt
    12481 ccccaaaagt ataatttaga ttatattttt tcttctaatg atattttata ggaaaatcaa
    12541 cataatttca ttaatatttt aacaaaatat ttttctaaat ctactataaa tttttatcct
    12601 ataaattttt cttatacact atataaaaaa ttagacttag acaaaacgct tttttcgccg
    12661 taatctataa ttagaaacag gaaaacctaa agcattagga ataaaagtaa ttgaaatact
    12721 taaaaacaag cttgtattaa aaaagtactt tattatagtg ttccctaatt tctttattca
    12781 gaaaattttt aattatcatt caagaaaggt aactattaga tatgaaagat gtagtaatcg
    12841 tatcagcagt cagaacacct ataggaagtt ttggaggtgt atttaaaaat acttcagctg
    12901 ttcaacttgg aactatcgct gttaaagaag cgatatcacg tgtaagactt aatttatctg
    12961 aaatagatga agtaataatt ggaaatgttc ttcaaacagg gcttggacag aatgttgcta
    13021 gacaaattgc tataaatgca ggtataccaa attctgttcc aagctacaca gtaaataaat
    13081 tatgtggttc agggcttaaa agtgtccagc tagctgccca atcaatcaca tctggtgaaa
    13141 atgatgtggt aattgcaggt ggtactgaaa atatgagcca agctccttat atcgttccaa
    13201 ctgctcgctt tggtagtaaa atgggtgaca taaccatggt agatagtatg ctaacagatg
    13261 gccttattga tgctttcaat cagtatcata tgggaataac tgcagaaaat attgcaacta
    13321 aatttgaatt tactagagaa atgcaagata aattggcatt agaaagtcaa aataaggctg
    13381 agaatgctat aaaaaacaac cgctttaaag aagaaatcgt tccagtagat attttaatac
    13441 gtcgaggaaa aatagaaact attgataaag atgaatatcc taaattaggt atgacatttg
    13501 aaggtttagc taaacttaaa cctgcattca aaaaagatgg tactgtaaca gctggaaatg
    13561 cctcaggtat taatgatggt gcggctatgt taatactaat gagccaacaa aaagcagacg
    13621 aactaggtat tagaccactt gctaaaataa aatcctatgc ttcagctggt gttgaacctg
    13681 aagtcatggg tactggtcca atccctgcaa ctagaaaagc actgaaaaaa gctggactta
    13741 gcatcaatga tatagactta atagaagcaa acgaagcgtt tgctgcccaa gcattagctg
    13801 ttaaaaatga acttcaaata gattcatcaa agttaaatgt taatggtggt gcaatagcat
    13861 taggacatcc aataggagca agtggagcta gaatacttgt taccttaatt tatgaaatgc
    13921 aaaaacgtaa agttgaaact ggacttgcaa cactttgtat tggtggtggt caaggaatat
    13981 caatggtggt ttcacgatag gaggaaatat gaataaaata gttagcattg atgaagctct
    14041 atctcatgta aaagatggag atacaataat ggttggtgga ttcatggcca atggttcacc
    14101 agaaaattta attgattatt tatgtagtaa aaatataaaa gatttgacac taatttgcaa
    14161 tgatactggc tttattgaca aaggagttgg caaaatggtt gtaaataagc aatttaaaaa
    14221 gataattgct tctcatgtag gattaaataa agaaactgga agacaaatga acaacaatga
    14281 aacagatgtt gagttagttc ctcagggaac tcttgctgaa caaatacgtg cagctggtta
    14341 tggtctagga ggtatactca caccgactgg tattggtact cttgttgagg agggaaagca
    14401 aaaaattgtt gtcgatagca aagaatacct tcttgaaaaa ccaatttatg cagatgttgc
    14461 tcttctattt gcatcaaagg tcgataaagc aggtaatcta gtttataaag gctctatgaa
    14521 caattttaat aatttaatgg catctgctgc caaaattaca atagttgaag cagatgaaat
    14581 cgttgaaatt ggtgatattg acccaaatga agttaataca ccaggtattt ttgtgaatta
    14641 catcgttgaa ggaggtaatt tataatggat aaattagaaa tgcaagaata tattgcaaat
    14701 agagtatcta aagaattaaa agacggagcc gttgtaaacc ttggaatagg tctaccaacc
    14761 aaagtagcaa attatattcc ggataatgta aacgttatct tacaatctga aaatggattt
    14821 ttaggtttag gttcagccga agatggaaat tcaagtgatg aaactattgt aaatgcaggt
    14881 ggtcaaccag ttactatact tcctggagga tgtttctttg atagtgctac ttcttttgga
    14941 ataatcagag gaggtcatgt tgatataact gttcttggcg cacttcaggt tgacaaatat
    15001 ggcaatatag caaactatat gattccaggt aagatggttc caggtatggg tggagctatg
    15061 gacctagtaa ctggagctaa aaaagttatt gtagcaatgg aacatacttc aaaaggttct
    15121 gcaaaaatat taaataattg tactcttcca cttactgcaa caaatgctgt agatttgata
    15181 gttactgaaa tgggagttat ggaagtaaca tcagatggaa ttttacttaa agaaataaat
    15241 ccagctttca cattagatga tgttatttct gctactgaag ctcctttaat attgtcagat
    15301 tcattaaatg gaaaaatatc tgttgtttaa aataattaaa actagaaaat aaaaatctct
    15361 tagacgtatt acatataaat ttgtattgct taaaatatta atataaaaat aaaataaaat
    15421 tggaggaaat gaaaatggtt aaagataaag ttgtattcgt aacaggagct gcaagtggaa
    15481 taggtaaaca aatagctgaa tcgttcttaa aaaatggtgc taaagtaatg ttctctgata
    15541 taaatcaaga atctttaaat aataccataa cagagttaca aaaaaaaggt tatgattgca
    15601 tgagtgtaaa gtgtgatgta actaaggaag aagaaattaa tcacgctata gacaaaactg
    15661 ttgaaaagta tggcagatta gatatattaa taaacaatgc tggacttcaa catgtttcta
    15721 tgatagaaga tttcccaact gataaatttg aatttatgat aaaaataatg ctgacagcac
    15781 cttttatagc tactaaaaaa gcatttccta taatgaaaaa acaaggcttt ggtcgtataa
    15841 taaatatggc ctcaatcaat ggtgtaatag gatttgctgg aaaggctgct tataactcag
    15901 caaaacatgg acttattgga ctaacaaaag ttgctgctct tgaagctgcc gattctggta
    15961 taacagttaa tgctttatgc cctggatatg tagatactcc acttgtcaga ggtcagttca
    16021 atgacctttc aaaaactcgt aatataccat tggaaaatgt cttagaggaa gttttatacc
    16081 cacttgtacc acaaaaacgc ctattagatg ttcaagaaat tgccgactat gctatgttct
    16141 tagcaagtga taatgctaag ggtattacag gacaagcttg cttactagat ggaggatata
    16201 ctgcacaata gttattatat ttagggcttg aagaaagtat tcttcaggtc cttctactaa
    16261 ggagggatat ttatgagtat tatcgcacca attggtataa ttattggtat catagctatt
    16321 atatattttt cagttaaaga aattcatata actatagctg ctccaatagc aactttaata
    16381 gttgttattt taaataaaat ggatgttgta acgtcaatgc taggtgctga caaaaacaat
    16441 tatatgggtg cacttggaaa ctatataatg agttattttg caatattctt attaggctca
    16501 atacttgcta aatttatgga agaaagtgga gctactgtat caatagctga ttttatttta
    16561 aataaatttg gctctaatca tccttataga gttttagttg ctatatttat agtaagtttt
    16621 atattaacgt atggtggtat aagcctattt gtagttatgt ttgcggtcat tccacttgca
    16681 agaactctat ttaaaaaact tgatatttct tggaacctta ttcagatacc actttggctt
    16741 ggtattgcaa caattactat gactataatt cctggaacac cagccattca aaatgttata
    16801 cctattcaat atctaaatac gtctcttact gcagctacta taccaagtat tgtaggtagt
    16861 ataggttgtg ctacatttgg acttgtctat atgaaatatg ctctaaacaa aagtattaga
    16921 aaaggtgaaa cttatgcaac ttacacttca gaattagaag aagaatcttt agatagagaa
    16981 ttaccaggat tttttgcaag tatttttcct ctattagcac tcgtaattat agctataact
    17041 ggaagtactt ttggttcaga atttatgaaa aaaaatgtaa tttacatagc tttaatagtt
    17101 gcaattcttt tatctgcttt tttattccat aaatttctat catctaagat acagacattg
    17161 agcataggtg ctagtggttc tataggtcct atatttgcta catcttctgc agttgcattt
    17221 ggagcagtaa taatggctgc acctggtttt gatgtattta gcaaacttat aatgtcaata
    17281 ccaggtagtc cacttataag tcttactgtt ttgacttcaa caatgtcagc aataacaggg
    17341 tcctcttctg gtgcattagg aatagtaatg ccaaactttg cagattatta tttaagtact
    17401 ggtctaaatc cagaattaat tcaccgtgtt gctacaatag cctcaaatat actaactata
    17461 gttccacaaa gtggtgtttt tcttacattc ttaagcttat ctaagttaac acataaaact
    17521 ggatttaaag aaactttcat tgtagttgca gttggttcac taatagcaga aattattgta
    17581 attacattag gattactaat gtattaatat aaaacaaatt aaaattcata atatgaaagg
    17641 tggtgtctca aagtgaactt tttacttaat gagacactct ttttatatga aataacataa
    17701 gcctatcatt tcaacaataa aaaagaacct tatctctatt ttgagacaga ccctttttat
    17761 ttcttaaaga tttaaataat tataatttgt tgctaatacg aaagcatcta ctgaataaat
    17821 ttaaccaaat aaaaaaaaat ttatcataac tataaattta cgttatggaa ttattataaa
    17881 aatattattt tctctaacta agtaattcca tactatacag gcttttaata caaattgtca
    17941 aacacactga aatataccta tttattccat atatatcttt tttacatatc tttacagtaa
    18001 aaaaagtctt aatcttatgt tagtatagat tcacgggtaa ccccctacac taaaggttac
    18061 aaatcagtaa catttttaga atttttagat taagaggtgt ttaatgatga gaagaaatat
    18121 accaacaaaa ttaaaaacta tattagcttt aggagtgtta actttagcaa cagcttcaag
    18181 tgctgttact gcaagtgctt catcacttga aaataatgta aatataagta acaaaagttt
    18241 agtatatagt aatactaaat acaagttttc tgcaaaacca aattgttctg taaaaccaga
    18301 acttaagcct gataaagata actcttgtaa ggataagaat cataatgaca agaattgcaa
    18361 caacactaat aaacctgagg acaacaataa ttcagactct actaataagc ctgataacaa
    18421 taaacctgag tctaataaac ctgaagacaa caataattca ggctctacta ataaacctga
    18481 taataataaa cctgagtcta ataaacctga agacaacaat aattcaggct ctactgataa
    18541 acctgataac aataaacctg agtctaataa acctgaagac aacaataatt cagactctac
    18601 taacaaacct gatgataata acaactcagg ctctacaagt gaaagttttt cagcttacca
    18661 aaaagaagtt gttgacttag taaatataga aagagctaaa gctggattaa atcctcttac
    18721 tcttgattct tctatatcaa atgttgctac aaaaaaatct caagatatga ttgataacaa
    18781 ctatttttca cacaattctc caacttatgg ttctccattt gacatgttga aaaaatttgg
    18841 aataagctat aagacagcag gtgaaaatat agctatgggt caaaaaactc ctaaagaagt
    18901 tgtaaacgca tggatgaatt ctgaaggtca cagaaaaaat ataatgaatc caaatttcag
    18961 taaaataggt gttggagtag cacaaaaaag tggtggttca atttattgga cacaaatctt
    19021 tgtaggataa aatactcttt ttccatacat atcaaaagag tatctctaag tgataaaaaa
    19081 cacaaatgag atactctttt gtatctattt taaaattatc aaatccaaat atacatacta
    19141 taaatattat ttgttctctt aattttagtg tgtagagcaa tatataaaaa gtattggcaa
    19201 gatgaaaatg gaaaacttaa aaaagatatt taataggtaa aaacaaaatc ttaatatctt
    19261 cataaaaaaa ggctgtctca aaatagagat aaggcctttt tcattgttga aatgattaaa
    19321 atatatttga tttcatacaa aaagagtgcc tcatgaacta aaaagctcac tttgagacac
    19381 tctcttttat caattctata taagcattat aatactttaa tattattcag ctaatttttt
    19441 gtatccttca tatctttctt tagcatcagc ttcagcttta gcaaataatc catcagcttc
    19501 ctctgggaat tgtttagcta tagcagaata tcttacttgt gcaagtatga aatctttgaa
    19561 gcttgcagtt ggttcttttg aatctaaagt gaatgggttt ttaccttcag cttttacttc
    19621 tggattgaat ctgtataaat gccagtatcc gcaagcaaca gcttgtgctt cattagctac
    19681 agttcttccc ataccttctt ttaatccatg gtttatacat ggagcataag ctattattag
    19741 agatggccca tcatgttttt cagcttctaa aacggctttg ataaattgat ttttatcagc
    19801 acccatagca acttgagcta catatacgtt tccatatgtc atagccatca ttcctaaatc
    19861 tttctttcta gattttttac ctgaagcagc aaacttagcc atagcagcag ctggagtagc
    19921 tttagaagct tgacctcctg tatttgaata tacttctgta tcaaatacaa gtacgtttac
    19981 atcttcacca gatgcaagaa catggtctaa tccaccataa ccgatatcat atgcccaacc
    20041 gtctccacct agtatccatt gagatctctt aactagataa tcttttctat cttttatagc
    20101 ttttactaat tctttagctt tttcatctgt acaatcacaa gctggttttt ctaataattc
    20161 aacaacctta gcacttgcta ctttagaagc ttctccatca tttctagaat ctaaccactc
    20221 tgtaaatact gctttagcat cttcacagat atccatagaa actaactctg tcatagcatc
    20281 tactatttta tttcttattt gcttaacagc tgtgtacata ccaaatccat attcagcatt
    20341 atcctcgaat aaagaatttg cccaagatgg acctttacct tcatggttac aagtataagg
    20401 aattgatggt gcagaagctc cccatataga agaacatcca gtagcattag ctatcatcat
    20461 tctatctcca tataattgtg taactacttt aatgtacgca gtttctccac atccagcaca
    20521 tgctcctgaa aactccatta atggttgtct aaattgactt cctttaacag tatttgcagg
    20581 cataacatca acttttggag ctacttcatc aggatttact gcaaatgccc aattatctaa
    20641 ctctgtatca agttgagtat ctattggctt cataactaaa gctttttctt tagctggaca
    20701 tatgtcagca cagtttccac atcctgtaca gtcaagtgga ctaacttgaa ttctatattt
    20761 taatccttct aatcctttac ctaatgcttt tttagcttca aatccttctg gagcattagc
    20821 taattcttct tcagtaacta atactggtct tatacatgca tgaggacata taaaagaaca
    20881 ttggttacat tgaatacagt tatctattat ccattcagga acatcaacag ctataccacg
    20941 tttttcataa gctgcagtac cacaagggaa tgttccatct tctataccat tgaatgtact
    21001 tactggtaag ttgtttcctt cttgtgcagt cattggtctt agtatatttt ttataaattc
    21061 tggttcttta acttctcctt tatctgcatc aacagcattt tcccaagaag ctggaacttc
    21121 aactttaact aaagcattta ttcctgcatc aacagcagca tagttcatat taactatttt
    21181 ttcacctttt ttaccataag ctttaactat agaatctttt aagtatttaa cagcatcttc
    21241 ttctggtatt atttcagcta atttaaagaa tgcagattgc attatcatgt ttattctgtt
    21301 tccaagacct atttcttgtc ctagtttaac agcgttaact gtatagaatt ttatatcatt
    21361 tttagctata tattgtttca tatgagctgg taaatgttct tcaagtcctg cttggtccca
    21421 tatagtgtta agtacaaatg taccaccttt tttaagacct tttaataaat catattgata
    21481 aacatatgat tgtttatgac aggcaatata atcagcttca tctattaaat aagtagctct
    21541 tattggagta tcaccaaatc ttaagtgaga catagttata ccaccagatt ttttagagtc
    21601 ataatcaaag taagcttgag catatttttc agtatggtca ccgattattt tgatagcttg
    21661 tttattagct ccaacagtac cgtcagaacc taatccccag aacttacatc ttatagttcc
    21721 tttagaagct atttttattg gctcagatgg agttaaagaa gtattagtta catcatcaac
    21781 tatacctact gtaaatccat ttttaggttg ttctgaaact aagttatcaa aaacagtttt
    21841 tatatctgaa ggagtagtat cttttgaacc taatccgtat cttccaccta ttatcatagg
    21901 agcattttct tttccataga atacatcacg tacatctaag tataatggtt caccagttga
    21961 acctggctct ttagttctat caagtacaca tattctttca acagtagatg gcatagcatc
    22021 taagaaatgt ttcattgaga atggtctata taaatgaact ttaactaaac catatttttc
    22081 tcccttagca tttaagtaat ctatagtttc ttctattgtt tctgttactg aacccatagc
    22141 tattaatacg tatttagcat cttctgctcc atagtaatca aataaactat gttttctacc
    22201 tgttaaatta ctcatttttt ccatgtattt ttcaactata cctactatat tttgatagta
    22261 tttattagaa gcttctctag tttggaaata tatatctggg ttttgagcag taccacgagc
    22321 aactggatga tttggagata aagcattatc tctaaatgct tgaactgctt caaagtttaa
    22381 taaacttgca taatcttcat tttctagtaa ttcaactttt tggatttcat gtgaagttct
    22441 gaatccatca aagaaatgta taaatggtaa tctaccttct attgcagcta aatgtgcaac
    22501 aggtgctata tcagctactt cttgaactga accagaagct aataatacac atccagtttg
    22561 tctagcagcc ataacgtctt ggtgatcccc aaatattgat aacgcttgag atgctaaagc
    22621 acgagcactt acatggaata ctcctggtaa taactctcct gcaactttat acatgttagg
    22681 tatcattaat aataaacctt gagatgctgt aaaagtagaa gttaaagctc ctccttgtaa
    22741 agaaccgtgg aatgttcctg atgcacctgc ttctgactgc atttcaacaa cattaactgt
    22801 ttgtccaaat atattttttc ttccttgtga tgcccactca tcaactactt cagccatagt
    22861 tgaagatggt gtgattggat atatagctgc tacatctgta aacgcatagg caacatgagc
    22921 tgcagctgta tttccatcaa gtgttttcat aaacttagcc ataatatgta tcctccttga
    22981 tagctacaat tgtacttgtt tataacaagt ctattttttt atatttacat ataatttaca
    23041 caacaaaaat ataaacacac atatagtcgc gcaaatactc tatcttctaa tataatacta
    23101 cagattagat aaaattcctt tgaaaattgt aaattaatta catatttaag tagatttttt
    23161 ttaaaattaa atatgttaag tttttttcat aattttgtat acaatatata ataaattaag
    23221 ttagactttt caaacttttt tatctatttg tttatattag tacaaaaagt aaaataatta
    23281 tctttattat atgtaaattt ttatatatat aattttacca tattacatca tatttttgta
    23341 agtttttatt acatttttta taatacccct ttattttata aattaatcta atcgattaaa
    23401 tttatcctat agaaatttat tacatttaaa aatatgctag caaataaaat atctactagc
    23461 ataaagtcta tttataatat taatcaacta ttaattctac taaatctcca tttttaacgc
    23521 ctgctgcatt tccctcttcc atatcaacat gcatttctag attaaagctg tcactagctc
    23581 ttactaatac attttcaaat actaatgctc ttggtccaga tgttttaact tttactattt
    23641 gcttatcagt tacaccaaat ttagcagcat cttcattgct catatgtata tgtctagaag
    23701 caactataac tccttctttc atttcaactt ctccagcagg tcctactaat ttaacacctg
    23761 gagttccagc tatatcacca gattctttta ttggtgcttt tactcctaat gggaaaccat
    23821 ctgctaaaga tatttcaact tgagtatgag ctctagctgg tcctagtact ctaactcctt
    23881 ttaaagttcc ttttggtcct actaaatcca ctttttcttc acaggcatat tgccctggtt
    23941 gagataatgg tttaaattta gttaactcat gacctgctcc aaataatacg tctatatcag
    24001 cttgactcaa atgaacatgt ttattagata atgctattgg taatttcatt gaaattccct
    24061 ccttttaatt atttaaatta ttgcaataca attatacata atattaccta caaaatcaaa
    24121 ttatattgaa tcaatatata tctttaaatt tttatatatt atatctttaa attcttgatt
    24181 atctatataa gatacatcat ctgtattgtt taaatatata ttgtgtaaaa gtttagtatt
    24241 tatactctta aagaaaaaat ccataacaat ctgtccacct ttaaactgtg aatcatatga
    24301 tttactccct cccactgcaa tatacattcc tatccgtttc ttacttctat caattgaagg
    24361 tttatttaat acatatttac ttgcatatat agcttgagtt ctatcaacaa cagtctttaa
    24421 tttagctgat attgaatcaa aatgtacagg acttattact atagttcctt ttgaatcatc
    24481 aaacatttta tataatggct tcatatcatc attaaacttg caataccctg ttttttcaca
    24541 aaatccacat gctgtgcaat attcaatatc catattgtag atattaaata ttttacaact
    24601 taatccacac tcttctagca tagcttttac ttcatttgct ataaaactgc aatttttatt
    24661 aattcttgga cttccattta ttattataat ctctggactt ttaagtcctt ttttatcgtt
    24721 tgaatccata atcaatctac ctatccttta acataactat taaaatatca tcatcataat
    24781 tatcttttga tgagaagttc tttaaatcca attttagatt ttctactatt aactgttgat
    24841 ttaatctaaa attttctttt agaaagtttt ctaatcggtt tataccatac tcttcttttg
    24901 atgaattttt tatctctaaa attccatcag tgtacataca taccattgca taatcttcca
    24961 ttgaaagagt attattttca taagtagcat cttccattat tccaataggt atgccctttt
    25021 tacaatttaa attattttct attgtgccat ctttctttac aactacagga ctatagtgac
    25081 ctgcattaga cacagtaatc atattttctt tagtatctat aatacctatc aaacaagttg
    25141 taaatactcc catcttgtca aattcatcat ataacatcct atttaaattt gtcattattt
    25201 ctcttggtgt attgtattga ttacataata ctttaaaagc accttttatc attgcaacaa
    25261 cataatttga taccattcca tgtcccatta catcagcaac aatataaact atatgttcat
    25321 catccacttt tgtagcataa taaaaatctc cacctactac ccttgctggt ttatggtaaa
    25381 aataaagttc tttctcatca ttacaatcca ttttaccttt atccataata agctgttgtt
    25441 gtttatttaa tatgttcaac tcgttttcaa gaactttgtg ttttacttcc ttttcattat
    25501 gttcatataa ttgcatacca acagaaactt gtttggacaa tatactaaga atatttaaat
    25561 catcactagt atagtttttg ctatttgcac aaactataca tcctatagta tcatttttat
    25621 cctgaagttt gtaatatata taactttcca aatttattcc cttttttgag catggtttta
    25681 aattatttag ataccccccc aatgactctt tctttaaaaa catatcaatc ataggatata
    25741 aatttgtttt ctttttttgc tcaacattat aacaactaac tatagcatcc ttattatgat
    25801 gactatttat aattattaaa gcttctttac tattagtaat attacacgct tgtttactta
    25861 aattttccaa aaagccatca atactcttat taaggtatag ttttttagta gcttcattta
    25921 tggcacttat tgagcctctt agtttatcaa ttaaaatttg tacattatta tttttattag
    25981 ttatttctat tgataaactt aagagtgatg caacagatga tataaaatct atatcttgtt
    26041 ctgttatata atcatcctct tttaaaaaac aagtcataaa tccaacaact acgttattta
    26101 ttataaaagg aaaaactatt ctcccaatat aaccttcagc ttttgctaaa tctcgttcac
    26161 cttctgctct agaatcttca aatacatttt ccacatatat tatctttttt tcatttacag
    26221 cctcatgtat atactctgga tatgtgtcaa aatcaatttt tgcaccttta agactattta
    26281 ttggaaaaat ttgaggtaca tattctaagg tttgcgaaca tactaagtag gcatatttag
    26341 aatcattttt ataaaataga ttcacacatg cctttgtagg gtgaacaact tctaacattt
    26401 tttctattat gtcatctttt atattaaaaa aatctgtaga ttcagtcacc atagcggata
    26461 tttccactaa attcttcatt ttataactgc cattagtcat actcaatttc tccccctata
    26521 tattaaacta tttattagta ttttaacata cattctttat agtaaaaaca aaaacaacga
    26581 caaaactagc aactaatatc cagttaaatc ctgtcaataa tcctataaga agtaatgtaa
    26641 ttaatgcatt tagacctgtt ctcccttttt catccataaa agttatttta gagatgtcga
    26701 tattattttt cacattattt atatgttctt ttacttgttc ttccatattt cttttatata
    26761 gtatataaac tacaccaact aaaaacactc ctatacctaa aaccatgttt aaattaaaag
    26821 ctaccataag caatattgaa ccaaaagcta actttaaaag cttaaaaaat acactatcta
    26881 tctttagagt tatatttctg atttctttac attcatctct taaagaatat ccacttgaat
    26941 ctaagttatt aatctttgtt tttacaattt cagaataaaa aagctttatt atcccattta
    27001 acattttcat cactcctaaa tattttacat atatttgtaa gttttacaat tataatattt
    27061 tttatacttg gtaataataa aaaataacaa atattttatg atttagtatt tattgtaaat
    27121 aagttattga agctttatta gttaagtata gatttttaat aaatattttt ataattagaa
    27181 ggaagtggtt ttgtgcaaat agaacaactc gatttagaaa cgagaaataa aatatactgc
    27241 tatactaaaa aaattcttag aaaatatcaa aaaggcataa catctggaaa attaacagca
    27301 gataaatttg cagataatat attgtctcaa gattttatca gtagtatact taatgaaaaa
    27361 atagtcaatg agacaaactt taagatatct tatagaaact atatagaaac tttaataaat
    27421 attcaaaatg aaaatttatc taacttaaga aaaaaatcta ctaaaactgc aaaatgtttc
    27481 aatatctctc aaataaccca attaaaaaac ttactaagta atactggtta taatttatta
    27541 attccatata aatacttaac tgctagagat atagaaggaa tcgttactct tattaatact
    27601 gggtctattg aacttggaaa tgagagaatc tacaattata tatcaaaagc ataataattt
    27661 aaacctatta taaaaaggca atttatttaa taaaaaatat ttttaatatt aaataaattg
    27721 cctttttaat tttggactaa atactgctat ccaagtgctt tttctagttt agataaatgt
    27781 tgtattggat aatattttag tagctcttta aattgttcta ctgataaacc ttctgttgtt
    27841 gtatatattc ttattttttc atctaagtca ctattaataa atttgttttt taaatcctca
    27901 aaatttacac tactcatttt acatcactcc ttatgtaaca tattcatttg tattatttta
    27961 tccaaataca taaaatttat tacataaaaa tatatctatt taataattta ataaaaaaaa
    28021 gctatctcaa tttagaaatt actttctata tttgagatag ctctagctaa actaatagcc
    28081 aatagttata aataattatt tataactatc tgttgctttt tccagccatt tgttgttcag
    28141 ccatttcaac taatttttta gtcatgtatc cacctacata accattttct cttgcagtaa
    28201 ggtttccttt atcaacttgt tgataattac tcataccaag ttcattagct atttctaatt
    28261 tcatttggtt taaagcctct tttgctcctg gtactactgt tctgttgttg ttattgttac
    28321 ttgccatgtt gattaccttc cttctgttta atttattttg tgttgctgat aatattatgt
    28381 gattttaaac atttttaatt caggtaattt ttagcaatta aaacctgcca aatatccagt
    28441 agaaaaagct atttgcaaat tgaatccacc tgtatatgca tctatatcta ttacttctcc
    28501 agcaaaaaat aaaccttcta ctaattttga ttccattgtg cttgaattta tttcgtttac
    28561 ttttacacct ccagaagtta ttatagcttc ttcaataggt ctgtatctct ttactgtaaa
    28621 ctttaaattt tttaataaat ggactaagtt ttttctctgc tctctagaaa tttggtgaac
    28681 aaccgtattt ggattaattt cacttaagtt tattattata ggtattagtt tttttggaag
    28741 taaatcatct aatgcttttt caaatttttt atttgtatac ttctgaaaat ctttttgaac
    28801 tcttttatcc agtttttcct catctaatgc aagttttaaa tctaataata tagtgtaatt
    28861 ttctttcctt gtatctttca ttctacaact tgcagacttg attataggtc catctaaacc
    28921 aaatctagta aattcaagtt ctccaaaatc actataaact ttcttttgtt tgctattaaa
    28981 cacccttatt tcaacatttc tcaatgataa tttttctaaa tcttttgtaa aactttcttg
    29041 tacttctata ccaattaaag atggttttgt atctataatt gtgtgcccct gtgaaattgc
    29101 aaatttatat ccatcaccag tagaaccagt aagtggataa cttaatccac cagttgctac
    29161 tacaactgaa tcacacttaa tttctttttt atcgtttaaa attactttct caattttatt
    29221 atttttagat attatttttt ctactttcga atttaataat atatttactt tcttactttt
    29281 aagttgtctt tcaagagcat ttacaatatc aaatgcttta tcactctctg gaaaaaccct
    29341 ttttcctctt tctgttttag tctttactcc aagattatta aacatactta ttacatcatc
    29401 atttgtaaaa gtataaaaag cactataaag aaattttcca tttgtaggta cattttctat
    29461 taattcctct atttcacaag cgttagtaat attacatcta ccttttcctg ttattgcaag
    29521 tttttttcct aatttatgat ttttctcaat taatgttaca tcatagcctt tctcacatgc
    29581 agttgatgcg gctatcatcc ctgctggtcc accacctatt attactacct tcttcaaata
    29641 atcactcttt tctaccctta atttttttaa tttaaactat tgactctata ccttaaaatc
    29701 aatttaattt taaaagttta aataatgctg gagctcctgc tattccataa aaaacaacta
    29761 ctgtattctt aataaaattc ataaaaatta tactcatagt agagtcactt atatttaaag
    29821 tgactattaa cattgaaata aacaacttaa tgccaatata taacattcct attgttacta
    29881 tcccaaccaa aaacctacat attcttgaaa aaattatatt tttactcccc ttatttttat
    29941 tactaaaatt tatttttttg ttattattat ctgtattaaa tttaacaaat ctatcctcaa
    30001 ccatatatcc aaatacaaat cctactatta taccaaattt ttcaaaatat tcagggctat
    30061 ttagaaatat agccactatt ataaatggta taagtaaaac tacaagtaaa atataatttt
    30121 tattatcatc aatatagtca aatattttta caaatataac tgtaaaaaat attcctaata
    30181 tccaacccgc tatcacatct attggccaat gaacagctaa atacagccta gacaatccta
    30241 ctcctaaaac cataactgca cctattaaat gaagatatct ctttttaaat atagtcataa
    30301 tactaaccca aaatgttgtt gcagtttgag tatgagcact tggaaaagaa taaccaccag
    30361 cagtagaaac tctcaatgat tcaagacctt ttattcctat tggtctggga gcttttacaa
    30421 actctttgat gcctaaattt acaacaaaat ttccaattat agcaaacagt attttctgtc
    30481 cacatttttt atttatacac cagtatataa ttgttgtaat taatattaat aacggtgctt
    30541 ctgtgcttat tgtaaagaca agaaatataa agttcaaaat tggatttctt aaattttgga
    30601 aaaataataa tatatttaac tgaatatcca tatagatgtc tcctttacta aaattttata
    30661 taattaacta aaaagcacta actcccttag aaattagtgc ttttgattca catttaaatc
    30721 tattctacat cctcaactat acctatataa ggtaaatttc tatacttttc agcatagtct
    30781 attccataac caacaataaa tttgtcttct ataacaaatc caacatagtc tacaggtata
    30841 ttagcttttc ttctctgagg tttgtcaagt agagtacata gttttaaaga ttttggattt
    30901 ctagttttta aagctgcaac tagattactt aatgttaaac cagaatctat tatatcctct
    30961 acaatcaaca cattctttcc ttcaatatct acatctaaat cttttaatat tttaactgtt
    31021 ccagaagact ctgttgagtt tccataactt gtaacactca taaaatctat atttacatcc
    31081 aaatctatac atcttattaa atctgaaaca aatacactag ctcctttaag tatacctacc
    31141 actagtaaat cttcgccttt aaagtcttct tctatctttt ttcctaattc ataaactttt
    31201 tcttttattt gttcttctgt caacattttt cctgtaactt tgtacatttt gtatcctccc
    31261 gctttccttc tattaactta tatattatag cataccaaaa tatcaattaa aatctttttc
    31321 atcattttta aacctaaatt aacagcttat ccatactttt ttgctttttc cattaaaaat
    31381 ttggaataac gattcctttg tattataatc tatatgtaca tatattaatc agttattttt
    31441 atcgattata taattttatt tttggaggta cttttatgag cacaaatcat ggagctaatt
    31501 tatatagttt atctagtaag tatggctttt caaaggaaga atttatggat tttagctcaa
    31561 atataaatcc ttttggtaca tcaagtttag ccaaacaata cattgtaaac aatatagata
    31621 tggtatctat gtatcctgac cctgattaca tagacttaaa aacttctata tccaattact
    31681 gcaaatgttc tatagataat atagtacttg gcagtggagc tacagaacta atttcatcat
    31741 ttatacatac aattaatcct aaacaggctc ttttactctc tccagcctat tcagagtatg
    31801 aaaaagagtt atctaaaatt aattgttcta tagaaaaata ctttgcaaaa gaagaagata
    31861 attttcatat aaatttagaa aatcttataa aaaccatcaa tgctaaagat tatgatttgg
    31921 tagtaatctg taatcctagt aatccaactg gatttgcatt tactaaagta gaagtgagag
    31981 aaattttaaa aaatacagac tcatttttaa tgattgatga gacttatgta gagtttactg
    32041 atacagatac atactcttgt actcaattag ttgatgatta ttctaattta ttcgttataa
    32101 gaggtacatc aaaatttttt tcaacgcctg gaataaggct tggatatggc cttatatcta
    32161 acactaatgt caaaaatgaa ataaataaaa atctagattt atggaatata aacataatag
    32221 cttctaaaat gggggaaatc atgcttagtg atttagattt tatatcaaat actatttcct
    32281 taatgaacac tgaaagagat tacctgctta aagagcttaa gaatataaaa agcttagata
    32341 tatacaatac taaaggtaat ttcatattgt gtaaaatcaa gacaaaggaa ttgactgcta
    32401 aatctcttcg agagcaactg cttccacaaa aaataataat aagagattgt tgttcatttg
    32461 aaggtctaga tgagtatttc tttagagtat gtatattaaa acctaatgaa aataaattat
    32521 taatatcatc tcttaaagca atttttaaat aaaaaaatca ctttatttta taatttttta
    32581 taccataaaa aatgagctat ctctatttga gataactcat ttttgattat taaaaaacta
    32641 agcactttgc tcttctttat ttcctttttt actcattcca tcatttattt tatctactac
    32701 tactgctatt gcattatctc ctgatacatt acatgctgta ccaaaactgt cttgagttaa
    32761 gtataaagct atcattaaac ttgcaagccc accatctgat ggtattccaa ccattggtag
    32821 gaaaggaagt gcactcatta tagccccacc tggtgctcct ggtgctgcaa ccattgctat
    32881 acctaacatt gcaatataag ggaataatac acctattcca tgtggcatac cattcatcat
    32941 taaaactgca gttgtaaatc ctgttattga tatcatacta cctgataaat gtattgttgc
    33001 acaaagaggt acaacaaatt ctcttatttg cttacttact ccattttttt ctgcacaacc
    33061 aacattaact ggtatagttg ctgctgaaga ttgagttcct atagctgtta aatatccagg
    33121 tacttgattt tttaacattc ttactggatt ttttcctgaa aatacacctg ctgctgtgaa
    33181 ttggcatgcc atatatacaa aatgtaaagg tattactact aagtatactt tccagaatac
    33241 ccctaatatt gtccatactt cccctgctat agttatattt gcaaaagttc ctgctatgta
    33301 taaaggtaat aaaggtataa tcactgttga caacgttttc gttacgattc cttgaaattc
    33361 ttggaagaaa ttaaataaag tctctccttt tccatgattt cttaaagctg atattccaag
    33421 tcccattaca aatgcaaata cgattgcagc tgttacatct accataggct ctaatggaat
    33481 tgtgaatatt ggagctaaca ttcctgcctc tgggtctcca atttgacttg caacacttgc
    33541 atcaataaaa tttgggaaga ttaatgtaga tactgcaaat gataataatc ctgctattaa
    33601 ggtagaacca tatgctattc ccgtagttat ccccaataac tttcctgcac ctgttgctaa
    33661 atcagctata cctgctgtta caaaacctat aattataaat ggtataacaa attttaggaa
    33721 tgctgagaat aaagtactaa ctgttactaa tatttgtact attatttctg gtgcataact
    33781 tccgattaaa ataccgacta tgataccgat gattagtttg ggcactaaac ctattttttt
    33841 cttcataata tttaccctcc ataacttttt taaattaagc attcttgcac attgaatact
    33901 tagttaaata ttggatatcc acttattatg ataatagagt tttacctctc cgtcaacaat
    33961 ttaaccattt tttgttaata aaaaaaagaa taacatatat aaattgttat tcttcttcat
    34021 cttttataga aaattctaca ttttcaatta tatttgaatg tttatctttt tttgcgataa
    34081 ctctatactt attaatttct tcattcacta tacctatact ccaaattaaa actatagcat
    34141 catctataaa accaaatgcc cctgcaatca tctctggaat tatatcaacc ccagatacaa
    34201 agtaggatat agtaaaaatt aatacagaaa ttacctttat tttatcaact atattgattc
    34261 ttttatctgt cataaaatca ggtagtttaa gtaaatcaaa tacaaaaacc aaaccaaatt
    34321 tagcattttt aaatctgacc aaaatttttt ttaaaatatc taaaactata ttaatgatta
    34381 tttttaaaaa ctccatattt atcacctctt ataattatag gttattctac attataaccg
    34441 atataattaa tttgatgaac atattagata taaattttat caattagttt atatttaaat
    34501 tataataatt caattcctgc actactacaa ttttgaatca tatcctgtgg ccatactcca
    34561 acttgtactt ctcctatatg agctttttgt aagaaataca tacatattct tgactgacca
    34621 attcctccac caattgtata tggaagttcg ccatttaaaa gcatcttatg atagtcaaac
    34681 tcttttctct cttcacatcc agcaagtttc aattgtctat caagagattc ttcatcaact
    34741 ctaattccca ttgaagataa ttctaatgct gaatctaaaa caggattgta aaataatata
    34801 tccccattca attcccaatc atcataatca ggacttcttc catcatgttt ttctcctgat
    34861 attaatgttt ttcctatttg agttataaat actgctttct tctcttttac aattttatct
    34921 tctctttcct ttggtgataa ttctggatac atatcttcta actcttgtga tgttataaaa
    34981 gttatttctt ctggtaatat ttcttttata ccttcataca tactacatac aaaaatttca
    35041 gtatctttaa atactccata caatcttttt acaacatctt ttagagtctt ttcatttcta
    35101 ctacttttat ctataaccaa ttcccaatcc cattggtcta cataaagaga atgtatattg
    35161 tctaactctt cattttttct tattgcattc atatctgtat ataaaccact accaacttca
    35221 aacccatatt ttttaagggc taatcttttc cattttgcaa gtgattgaac aatttctgca
    35281 tttttattca taaatggtat atcaaagctt acagcctttt ctccatttaa attatcattt
    35341 gcacctgttt ctggcaatac aaataacgga gaagaaactc ttataagatt taattcatta
    35401 cttagtctca tttcaaaaaa atcttttaat ttttttatag ctatttgtgt atcaattacg
    35461 ttcaaactac ttttataatc ctttggtatt attgtgctca tatcattcac tccctatatt
    35521 tatatatttt tccattaaaa aaacccttca tccctatata aaagggacga aaggttgctt
    35581 ttccgcggta ccaccctgat tagtttgaaa taaattcaaa cccagctcac aatgtacaga
    35641 tattactcga tacaccccaa tatgataacg gtttggttcc gactaagcct actcttttaa
    35701 aatttcggtt agcaactcca ggatgttctt cattatagac ttcatattgg tctcacacca
    35761 tcaccaactc gctttaatta cattctataa ctactcttcc tttcaaagtc tttaagagtt
    35821 taattcaata aaatttagct ctcttatgtt ttctatttat tcataatagt atgataagtg
    35881 attagttaaa atttgtcaag tttattattt ttttattttt aaacacttta taaattttta
    35941 cctatacttt ataaattttc aaatatagtc tttaatctgt ccatacattc ttttaataaa
    36001 gactgtggac aagctacatt tattctttca aatcctattc cttcttttcc aaatatatac
    36061 ccttcatcaa ataatacatt agctttttta aacattaaat cctcaagttc tacttcattt
    36121 aatccataag cagtaaaatc taaccatgct agatatgttc cttctggata aacaagtttt
    36181 acttttggta ggttttcttt taagtattca tcaataaatt gtatattatt atctaagtaa
    36241 tcatttaatt cattaagcca gtcttcactt tcattatatg ctgctattgt agctacaata
    36301 gcaaagggat ttggtgaagt tgcaactcca acagctgtaa cctcttcttg atatttcttt
    36361 tttagttcat cattattcac tattatatta gacaactgca ttcctgccag attaaatgtt
    36421 ttacttggag ctatacatgt aactatttta tccttgtact ctaggcataa tgactgtaaa
    36481 ggagtatgtt taaatccttt tctaattaaa tcagaatgta tctcatcagc tattatccat
    36541 aggtcatatt ttttacatat ttctacaacc ttttttaatt catcttctct ccaaactctt
    36601 ccaacagggt tgtgaggact acaaagtatc agtactttat tgttgctaca cttagctttt
    36661 tcttctaaat catcataatc catcctataa gtaccattct catatattag aggattgttt
    36721 acaacgtccc tattattgct ctttatctta gcttcaaatg ggtaatacac tggtttttgg
    36781 actattacac cttcattaga attagttaat attcttacta aaatagcaac agcaggtaca
    36841 acaccaggac taaaaataat atcttttcta tttatttccc agttaaatct ccttttatac
    36901 caatcacaaa ctgcattaaa gtatttatca ctatcatttg aactatatcc aaatatttta
    36961 tgttcaactt ttttacgtaa tgcttctatt attggttctg cacattcaaa atccatatca
    37021 gcaatccaaa aaggcaaacc atctttttgt gcttttgttg aacaattagt tctaaaatcc
    37081 cattttacac aatctgttcc tactctatct attactctgt caaaattata cttactcatt
    37141 ttttccctcc taagaataaa atttttataa ttgttaagtc tattactaga tgcactttgg
    37201 tattatttta caattcttaa tattataata atatcacttt tttgtccaat agatttaaaa
    37261 tataattact tttccatcat attttttcaa tattccaaaa tcagtttcat tatatcaaaa
    37321 taagattgct catattaatt attttctaat taacataagc aatctttttc tataacttct
    37381 ataattataa ttttaaatgt agaattatta gtttggatgt tatcccaatc tcttttcaac
    37441 caatctacac catatatcta caccatattc taaagctttc gtatcaaatg ttacatttgg
    37501 attatgaaat cctggtgtag catcagctcc taatccaata tatgtagttt tacattttag
    37561 tttttgtgta taataatgga agtcttctcc accagaattt ttaaatatac ctatagatgc
    37621 atcagctccc aatacatctt ttatagtttc ttcagccaag tttacaagtt cttcatcaaa
    37681 ttctgctgct ggtactccat ctttggaaat tagtacagct tctgcattta tagaacttac
    37741 aacagttttt acagcatgta ttgctttttc ttttaattct tctattatat cattactttc
    37801 tgcccttata tctaaagcca taaatgcttc atctggtata ttattataag tttctccctt
    37861 aactgatata tttgttactt ttatagaatt tggtatagct ggatttactc ttattgcatt
    37921 tattgcattc actgctaaac agacagcatc tactgcattt actcctaaat gtggtttaga
    37981 accatgacaa gtgatacctt taatcgtaaa ttccattata cagctagcac aatggtacat
    38041 tgcaggtatc gcctttccca acctacaatc ttgtccaggt cttaaatgca tccctaccat
    38101 ttcttctaca tcatccagca aaccagaatc aattactctt aatgctccat ataatttttc
    38161 ttcacctggt tgaaaaagta tttttaactt acctcttttt actaaatttc tctcttttat
    38221 taactttgca gctgttaaaa gcatagaagt atgaccatca tgaccacaag catgtatcat
    38281 tttcttttct ccatctatga taaattccaa tgcatccata tctgctctta tagcaaatac
    38341 tggtccttct tttttagaat ctatagttac tataatacca gttcctgcaa ctcctttatt
    38401 aatattttca aaccccattt tttcaagctc ttttaaaaca aactctgatg tcttatattc
    38461 ttcaaatcct ttttctggta tagtgtgtag ataatcatac actttatgta catactcaat
    38521 ataatctttg ttagttccca taatctagct cctccttaaa atttaaactt tccatttata
    38581 ttaaattttt attataatta cctatactac gaaataattc attataagca ttgcaataca
    38641 accatttaaa gttgctataa caaacatatg tggataatat ttagttttta actcagctgt
    38701 tcctaaaagt ctccccataa attgtatctg cgctcctgaa agcaataaca ttggcaatag
    38761 aattgcaaca tgtgcttgac ttatttgacc tgctgtaaat aaacctgcga ttactccaga
    38821 tgctcctccc attgatataa atattgtcat tatagctgtt gctgcaacac ctggaagtcc
    38881 aaagatactc attacaggtg taaatattgt ccctattaca tctaaaagac ctgttaaatt
    38941 gaacacagaa attatagcaa aaccgaagat gacatttgga gccattgaat ttgtacttat
    39001 ttggaatcca tttctagctc ctccaataaa cgcatctatc acatttcctt ttttaacttt
    39061 tacttctttt tttgctacct cactcatgtt aagcttcccc cttgtcaaat ttatttaaat
    39121 ataatctaag taagttcgcc ccaacaaact tcataattaa tattaaagca agtggtataa
    39181 atattggtac atctcctata aatggaaata gtgctgctcc agaagcaaag aaattcgtaa
    39241 ttgcagacgc acctgaaaat tgaaatgcac aaaatacagc tttttgtttt tcatctatat
    39301 acccatcatc tttaagttct ttagtcatag atgcagctgc atctgtagac tgtaaactag
    39361 ctatcaaagt tagtcctgca aatccaggta accctaataa tggctttaat agtggactaa
    39421 gaagcttttg agctgccttc attccatcta agtggtctat aactctaact actccaagtg
    39481 caaacataac tgatggtatt aatgtcaatg catatagcca tccatctcga gggccagtac
    39541 ctccatcacc tctaaagttt gcagctaatg tacctacacc atcattaatt gttcccaatg
    39601 ttccaaaaga acctaataca ttgttaaaat ccaatacttt taatggtcct ggtgcatctt
    39661 taaatattcc tgaaaaaaat aatgctccca atattaatgc tacataccca taccatttta
    39721 cctcaacttt ttctgttgtt gattctaatt tactcatata aattctccct cctgtttaaa
    39781 tgaattttat tagtatactt gtttaataag caaaacttat gccaatacct taatgataaa
    39841 gcctttgtat ttattttttt attgtaaatt tattccagcg attaaatagc ttataattac
    39901 ttacttttca agtttaaaaa tataatattt ttttattaat tatttaaaat atacattaca
    39961 atataaaaag ctattttcct tatcttgcaa aaataatttt aaaataggtt tttataggat
    40021 aaaatcctaa aaaaacctat tatttaatca aatatataaa tttatactac tatttgttgt
    40081 ataatataac ttgccttata ctttaaatac tttaaaaata aatactattt tgttaggaga
    40141 aataaaatgg ataaaaagaa gttaatcctt ataacatatg ataaattgaa tagtgaccac
    40201 tacaaagaag aattaacaaa tttttttgga gacgaaataa tcatagaaac acaaaatata
    40261 cttgatggaa taaaagaaaa tttagaggga gatgtagtgc tttctctatc acctttaact
    40321 agtaattttt taataaaaca ttttaaggaa gatattgaaa ttattcatgg aacgaaagca
    40381 ctttcaaaac ttggatatga aaaaatgatg aaactacctc caggtacaaa atcgttactt
    40441 atgacaacca ataaaacttc agcatttgag atggctactt atctatataa aattggtata
    40501 aatcatatag attttgtacc gacatatcca gattgtgatg aaatatatga tttagataca
    40561 gctataacac caggtcagat tagatttata ccaaaataca taaaaaatat tgttgattta
    40621 ggttggagaa aaatctcatt agatacatat atgtcattat tagtagtatt aaaattaaaa
    40681 aatgagaaat ttatagaaaa actatacaaa ttgtccaagg agacactaag tcatgatttc
    40741 ttaaatacat cattagataa tatatctaaa ttaaaaacta tactttacat gaccattgat
    40801 gaaataggtg atggtctaat atttttcaac acctttaaca aggttacttt tgtaaataag
    40861 tctttactta atatgttgga actagatgaa aaacttataa aatcaccttc cttaatggaa
    40921 tatatgccaa agagtttctt agataaaatt acaaagaact taaatataga taatatgatt
    40981 atatacatag acgaaataga taaaaaattc atactttcta aaaaaccatt ttatctttat
    41041 aaaaatatag agggttgctt aattacatta aaagatgtaa acaatataga aatattagaa
    41101 caaaaaataa gaagtgattc tgtcaaaaga ggttatgtag ctaaatataa atttaataat
    41161 attattggaa atagttctat tataaaagat tgtattaaaa aggcaaaaaa aatggctctt
    41221 acagataatc ctatattaat aacaggtgaa acaggaacag gtaaagaagc ctttactcag
    41281 tcaatacaca atcattcaaa tagaaaaaat aaaccttttg tagcaataaa ttgtgcttca
    41341 ttaccaagtg aattacttga aagtgaacta tttggatatg aagatggttc ttttactgga
    41401 gcaaaaaaag gtggaaaaaa aggattattc gaattagctc acactggcac tatattttta
    41461 gatgagatag gagatatgcc acatgattta caggttaaac ttttaagagt cttacaagaa
    41521 aaagaaatta gaaaaattgg tggtacaagt attattccaa ttgatgtgag aatattagct
    41581 gccacaaata aagatttaga gaaattaatt atagaaaata aatttaggat ggatttattt
    41641 tataggataa gtatgttcac tttagattta cctcctctta gaaagagatt agaagatata
    41701 cctcttcttt tagaatcatt tttaaaagaa cttccctata aaaacataaa actcgataag
    41761 agtcttttag aagctttaaa ttcatacact tggatgggga atattagaga actgagaaat
    41821 tgtgttgaat atatggctta catgggttcc aattatctta caataaatga tttacctcaa
    41881 aacatatctt caaaattaaa taataatcat atgtcaagta atatgtccat attcaatgat
    41941 ttaaaccaat atgacaaaaa catatgcatt tctatcctaa aatcattgca catgaaacca
    42001 atggggagga caaagttaat gaaatttatg gaatataatg tcacagaata cgaagtaaga
    42061 aatatgctag aatatttgac acgtaatgga tacttaattt ctagtaaagg aagaaaaggc
    42121 tcatcaataa ctgagaaagg cataaaaata atagaaaata atataattta aacctatagt
    42181 aattcatctt tactttatca aaatttatat ataattataa ttttttattt ttctataaat
    42241 tttttataaa ataataaaac ttatttatat taaaaacgaa gctatctcaa catagggata
    42301 agcttctttt taattgttaa aaagataaaa atatatttga tttaatagaa aaagagtgcc
    42361 tcataaacta aaaagttcat tttgagacac ttccttaata ttaataaatt aattggctta
    42421 aaattactga ttgataatta taaagtacct ctgttaaagg agtaaattct gaaccgattc
    42481 caaatcttaa ttctgtaaac gcctctaaat aatctgggtc acatacatct tcttgtgtta
    42541 aatcttgttt caccatttca gtagcaagat aaattgctac tgatggtaga aaagcatcca
    42601 ttggcatagg atatccacca agtctatttg acatgccaaa agccttagcc ttttcactta
    42661 tcatgtcgtt tattttatca taattttgag catctttttc agatattttt agacccataa
    42721 cactaggata tgtttgtatt ggtgatggat tgctttgctc tgcaactata tactttaatt
    42781 ctaaagcctt agtcaatata acttcatcca tatactcatt tacaccaaat acatttatat
    42841 cttttccata ttttttaact tgtttttcaa tatcttcatt taaaaactgc ttaactttgc
    42901 ttttgtcttc ttcagtattt atatttggag tattaacttg tacaaatggc aatcctatat
    42961 ttttgcaagt ctctttaatc atttctaatc ttttggctat attaacatct ttcaaatcat
    43021 ctgtagatgc ataatgaata aaagcttttg ctcccatttc ttttgaacgt tcagctaata
    43081 cttttcctct ttcctctgca ctaaccccta gattcacatc aacaaattgg cttaattggt
    43141 ttttatcatc tcccataggg gcagaaatag ttattatttc tggacgcttt tcctttacct
    43201 tttgtaaagc aggtaaaagc cctgcttggt cagttgaaac tacaatagcc tgaacttctt
    43261 tatcatcagc caactttact atcttattaa tagcactatc tatattagaa gtaaaattct
    43321 ctggcaatac aacatgcttt atagtttgtt taactttagt ttgaccatct ttgtctttat
    43381 caattttatt ctcttcctca tattcttttg ccatctcttc caccatattg tattgtactt
    43441 tattttcaga taatggttga gttaccactg caactttaaa atcatctata attttctctt
    43501 tatctttctt gcctacaagt aaactacaac ctgtaagtcc aataattaaa catatggata
    43561 gcataaatgc tgttattttt ctaagcatta attaaaacct cctaaatttg tacattctta
    43621 tcactattac taattttatc atacttcaaa gaaaaattat attataaaaa attatataca
    43681 tgctattata atccaatcta aaaagtacta tgggtagatt aggtttagat taaacctacc
    43741 catagtacta aaaattaata tattataaaa ttaaattaat tacaaattat tatgctactt
    43801 gtttgttgct ttttataaag ttaccaacta ttccacccaa tatagctggt attacccagt
    43861 taaagcccaa acctgcaagt ggcaagcttt gtataaatgg aacagctcct gtcattccat
    43921 ttatagctga taataaacta atcactaatg taacatatgc tgcaccttta aaagcattat
    43981 cattctttat atgtttacca gccaactcca taacaactat tgctaataaa acaggatata
    44041 tagtttgtag gattggaact gatatggcta ttatagaatc aacacctaag tttgatataa
    44101 gcatactagc tacacatata cctataacca gttgtttata actcgcctta cttattcctt
    44161 caaaataagt tgatattgat gctgttaaac ctgtagaagt agtcagacag gcaagtgcca
    44221 caattacacc taaaattacc tttcctactt gaccatataa caatatacta gttatttcaa
    44281 ctaataaaga tgcttgaggt atactagtat catataaagt tgaaactgtt gcacctaaat
    44341 aagtaagacc accatatact actgctagaa atacacaggc tacaaaacca gctttaatag
    44401 tcattcctat ttggtcttta tctgatgtat atcctttagc tattatagac atcaacatta
    44461 tagttgccat agatgctgca cctagagcgt ccattgtttg ataaccttgt gatatacctt
    44521 caccaaatag atttttatct atcatatgct caggactcat aggtcctgct ggagaaatta
    44581 taccctttac tattaaaaca aataatacta ataataacgc tggagttaaa tacttaccta
    44641 ttatgtctat aaccttagaa gacctaatag taaatgctaa agttaaccca aagaagatta
    44701 ttgaaaatac agctttagct actggaccat ggtctccaaa tataggcata atacccattt
    44761 catatgttgt tgcgcaagtt cttggtatta caagaagtgc taaacatgct agagcagcta
    44821 cactcattac tcttgctaac cctttaccag ctctcctaag tacatcatcc atgcttccct
    44881 tgcatcttgc tgttgctata ataacaagca ttgctatacc agcatctgca acaacaaatc
    44941 caataagagg aattaaccag tctttgccgg attccatccc taaaaatgga ggaaacataa
    45001 gatttcctgc accaaagaac atagaaaata aagcaaaacc aactataaaa atatctctca
    45061 tctgcttact cacactaaat tacccccttt gaaaatattt tgaatattaa gataatctta
    45121 ctttattttc agaaaaatgt caatctggta aaaattcaca cattgttaac gttttcccaa
    45181 tttattttat gaatattttt ttcattttat gataattccc atcaatttcg ttaaatcaat
    45241 tgcttgtgac acatcaaaaa ttgctcctct aatatcatct ataccaatct caattccctc
    45301 aatattatta gtagtaaaat ctatgccaga aagtttagtt ccattaaagt ttgctttttt
    45361 taaatttgta tactcaaata ctagattgtc accttttact tcttgaaata cagaattttc
    45421 aaaatttgaa tctaagatag aaactatctt aaatttagag aatgaaaagt tagaatactt
    45481 acctaaaaca tccttgaaca aaacgttttt aagtatacaa tcatcaaatc taactcctat
    45541 caatttacta tttttaaatt caactctgta tatacttcct ccatcaaaaa atgtatttga
    45601 taaattgcag ttttcaaata ccacatcaag taaatcaatc ttttttaaat tacaattttc
    45661 aaaagacaca tttctaaata tacaag
//
LOCUS       DAGSHT010000012        43937 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGSHT010000012 DAGSHT010000000
VERSION     DAGSHT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.013"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            183..1235
                     /gene="ytvI"
                     /locus_tag="MJB72_000601"
     CDS             183..1235
                     /gene="ytvI"
                     /locus_tag="MJB72_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY8261772.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1325..2551
                     /gene="pepT"
                     /locus_tag="MJB72_000602"
     CDS             1325..2551
                     /gene="pepT"
                     /locus_tag="MJB72_000602"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBY8261773.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2777..3829
                     /locus_tag="MJB72_000603"
     CDS             2777..3829
                     /locus_tag="MJB72_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBY8261774.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(4165..4734)
                     /gene="yfcE"
                     /locus_tag="MJB72_000604"
     CDS             complement(4165..4734)
                     /gene="yfcE"
                     /locus_tag="MJB72_000604"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBY8261775.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(4759..5559)
                     /locus_tag="MJB72_000605"
     CDS             complement(4759..5559)
                     /locus_tag="MJB72_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBY8261776.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            5694..6554
                     /locus_tag="MJB72_000606"
     CDS             5694..6554
                     /locus_tag="MJB72_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261777.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            6554..7240
                     /locus_tag="MJB72_000607"
     CDS             6554..7240
                     /locus_tag="MJB72_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY8261778.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            7292..7834
                     /locus_tag="MJB72_000608"
     CDS             7292..7834
                     /locus_tag="MJB72_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261779.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            7913..8380
                     /locus_tag="MJB72_000609"
     CDS             7913..8380
                     /locus_tag="MJB72_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBY8261780.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            9089..10225
                     /locus_tag="MJB72_000610"
     CDS             9089..10225
                     /locus_tag="MJB72_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBY8261781.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            10284..11066
                     /locus_tag="MJB72_000611"
     CDS             10284..11066
                     /locus_tag="MJB72_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY8261782.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            11085..12095
                     /locus_tag="MJB72_000612"
     CDS             11085..12095
                     /locus_tag="MJB72_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY8261783.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            12114..12911
                     /locus_tag="MJB72_000613"
     CDS             12114..12911
                     /locus_tag="MJB72_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBY8261784.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNSQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            13009..13854
                     /locus_tag="MJB72_000614"
     CDS             13009..13854
                     /locus_tag="MJB72_000614"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBY8261785.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            13941..15116
                     /locus_tag="MJB72_000615"
     CDS             13941..15116
                     /locus_tag="MJB72_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBY8261786.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            15311..16213
                     /locus_tag="MJB72_000616"
     CDS             15311..16213
                     /locus_tag="MJB72_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY8261787.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            16227..17231
                     /locus_tag="MJB72_000617"
     CDS             16227..17231
                     /locus_tag="MJB72_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBY8261788.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(17375..17602)
                     /gene="acpP"
                     /locus_tag="MJB72_000618"
     CDS             complement(17375..17602)
                     /gene="acpP"
                     /locus_tag="MJB72_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBY8261789.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            17774..18199
                     /locus_tag="MJB72_000619"
     CDS             17774..18199
                     /locus_tag="MJB72_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261790.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            18247..18453
                     /locus_tag="MJB72_000620"
     CDS             18247..18453
                     /locus_tag="MJB72_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261791.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            18635..18814
                     /locus_tag="MJB72_000621"
     CDS             18635..18814
                     /locus_tag="MJB72_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261792.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            18848..19048
                     /locus_tag="MJB72_000622"
     CDS             18848..19048
                     /locus_tag="MJB72_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261793.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            19301..20317
                     /gene="ccpA"
                     /locus_tag="MJB72_000623"
     CDS             19301..20317
                     /gene="ccpA"
                     /locus_tag="MJB72_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBY8261794.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(20440..20838)
                     /locus_tag="MJB72_000624"
     CDS             complement(20440..20838)
                     /locus_tag="MJB72_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261795.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            21050..21955
                     /locus_tag="MJB72_000625"
     CDS             21050..21955
                     /locus_tag="MJB72_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261796.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            22070..22250
                     /gene="ssrS"
                     /locus_tag="MJB72_000626"
     ncRNA           22070..22250
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MJB72_000626"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            22433..23650
                     /gene="cdeC"
                     /locus_tag="MJB72_000627"
     CDS             22433..23650
                     /gene="cdeC"
                     /locus_tag="MJB72_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBY8261797.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(23750..25072)
                     /locus_tag="MJB72_000628"
     CDS             complement(23750..25072)
                     /locus_tag="MJB72_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY8261798.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(25123..25917)
                     /locus_tag="MJB72_000629"
     CDS             complement(25123..25917)
                     /locus_tag="MJB72_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBY8261799.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEQMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            26027..27472
                     /locus_tag="MJB72_000630"
     CDS             26027..27472
                     /locus_tag="MJB72_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY8261800.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            27501..27710
                     /locus_tag="MJB72_000631"
     CDS             27501..27710
                     /locus_tag="MJB72_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBY8261801.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            27730..29124
                     /locus_tag="MJB72_000632"
     CDS             27730..29124
                     /locus_tag="MJB72_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY8261802.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            29364..30116
                     /locus_tag="MJB72_000633"
     CDS             29364..30116
                     /locus_tag="MJB72_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8261803.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            30142..30555
                     /locus_tag="MJB72_000634"
     CDS             30142..30555
                     /locus_tag="MJB72_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8261804.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            30559..31302
                     /locus_tag="MJB72_000635"
     CDS             30559..31302
                     /locus_tag="MJB72_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBY8261805.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            31302..31772
                     /locus_tag="MJB72_000636"
     CDS             31302..31772
                     /locus_tag="MJB72_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8261806.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            31789..32547
                     /locus_tag="MJB72_000637"
     CDS             31789..32547
                     /locus_tag="MJB72_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8261807.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            32531..33373
                     /locus_tag="MJB72_000638"
     CDS             32531..33373
                     /locus_tag="MJB72_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY8261808.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(33533..34438)
                     /locus_tag="MJB72_000639"
     CDS             complement(33533..34438)
                     /locus_tag="MJB72_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8261809.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            34800..35534
                     /locus_tag="MJB72_000640"
     CDS             34800..35534
                     /locus_tag="MJB72_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261810.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            35745..36926
                     /locus_tag="MJB72_000641"
     CDS             35745..36926
                     /locus_tag="MJB72_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBY8261811.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILDLEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            36910..37482
                     /gene="mobB"
                     /locus_tag="MJB72_000642"
     CDS             36910..37482
                     /gene="mobB"
                     /locus_tag="MJB72_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBY8261812.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            37601..37876
                     /locus_tag="MJB72_000643"
     CDS             37601..37876
                     /locus_tag="MJB72_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8261813.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            38131..39831
                     /locus_tag="MJB72_000644"
     CDS             38131..39831
                     /locus_tag="MJB72_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261814.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            40194..41381
                     /locus_tag="MJB72_000645"
     CDS             40194..41381
                     /locus_tag="MJB72_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261815.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            41441..42604
                     /locus_tag="MJB72_000646"
     CDS             41441..42604
                     /locus_tag="MJB72_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8261816.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            42755..43558
                     /gene="zupT"
                     /locus_tag="MJB72_000647"
     CDS             42755..43558
                     /gene="zupT"
                     /locus_tag="MJB72_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBY8261817.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            43653..>43937
                     /locus_tag="MJB72_000648"
     CDS             43653..>43937
                     /locus_tag="MJB72_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBY8261818.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWY"
BASE COUNT        17129 a         4748 c         7865 g        14195 t
ORIGIN      
        1 aaatagaatt aaaaaaataa gaatatatac ataatgtaat aatgttctta aatttattag
       61 aagtgtataa atataaaaga tatcttttat atttatacac ttctttttat tttgtattca
      121 aataaaacta gtatgaaatg gacaaaataa aaatataaat tagaagaaag gagattttta
      181 agatgagcat actagaaaat aattttttat acaaattaaa aaacaacact atcttttttt
      241 gtatatatac attgttattt atttttattt acaaagcgtt tccatacata gctccatttt
      301 ttctaggtgc tataatagca cttatgatta atcctatatc acaaaagtta gaaaataagt
      361 ttcgtataaa taaaggcatc tctactcttg ttctaagttt tcttgcagtg gctatagtca
      421 gtacagttac tacaataata gtaataaatt caatgaaaga attaatggga tttttaaata
      481 atatatctgc taatcctgag gatataagta atactattat gtatttttta aataaaataa
      541 atgactttat gaaatcattt caagaaatag ccaattttga tgtagaacaa ttagtaaaca
      601 aattcagtgg tgaagtaatg caaattacaa aaaatcttct tactagtata cttggattgg
      661 ctacttctat tccatatatt ataatattta taattacctt gtttattgct acgtacttta
      721 tagcaaaaga ccttgataag attgaaaata gtttttataa tatgtttact gttgatgtta
      781 gaaagaaggt aaaaaacgta aagaaagagg cagggctttc acttgttgga tatataaggg
      841 cctatacaat acttatggct ataacatttt ttgcaatctg gggaagtttt gctttatttg
      901 gattgaaata cggtctaata gtagggtttg taggagcttt aatggattta attccattcc
      961 taggtataat aacaatatat cttccagtaa ttgtttatta ttttcttata aaaaattatt
     1021 ttatagctat aagtatgact gtgatatttt ttgttttgtc tttgattaga gaaatacttg
     1081 aacctaaact agtatcagta aatgttggac taaatccatt agctacccta gcagcgattt
     1141 ttattggaat ccaagtaaaa ggaataattg gagtaatttt ttgtttgggt ttggtatgta
     1201 tgcatgatat acttaagaag gtggatattt tttaattata ataattgata gattttttaa
     1261 aaataaaatt ttattcacag gtaaataaaa aaatattaat cttattaaga aagagggtat
     1321 atacgtgaaa caaaaagtag tggaaagatt tttaaaatat gtatcatttg atacaacatc
     1381 taattctcag tgtgagaact gtccatcttc agaaggtcaa agagttttag ctaagtatat
     1441 agttgaagag ctaaaaacaa tgggtgttga tgatgtgagt ttagatgaaa atagttatat
     1501 aatggctact ttaaaaggaa atactgatgg tgtggatact ataggattca tatcacattt
     1561 agatacaatt gaagatgtta gtggaaaaga tattaaacca agaataatag aaaattatga
     1621 cggaaaagac atagttttaa atgaagcgtt aaatgttatt acgtatgtaa aggattctcc
     1681 agagttggaa gaatttaaag gtgacgattt aatagtcact gatggaacaa ctctacttgg
     1741 ctctgatgat aaagctggta tagctgagat agtgactgca atagagtatc taataaatca
     1801 tccagaaatt aagcatggag atataaaaat aggatttaca ccagatgaag aaattggtag
     1861 aggtgctgat ttatttgatg ttgaaaaatt tggagcaaaa tatgcatata ctttagatgg
     1921 aggaatagtt ggagaacttg aatgtgaaaa ttttaatgct gcaaatgcaa ccattactat
     1981 acatggtaga aatgttcatc caggttcagc taaaaataaa atggtaaatg ctatacatat
     2041 agctgctgaa atatcagaaa tgttcccagc agatgaaaga ccagaaacaa cagaaggata
     2101 tgaaggattt tggcatttaa attctatagg tggaaatgtc gagaatgtaa gtatggctta
     2161 tattattaga gaccattgta aagaaaaatt tgaaaataga aaatctatta tgatagagaa
     2221 tatagaaaaa ataaataaga aatacgataa tagagtggaa ttggatttaa aagactctta
     2281 ttacaatatg aaagaaaaga ttgagccagt tatgtttata gttgatatag ctaaagaagc
     2341 tatggaggaa ttaggtataa aaccacgttt agttcctgta agaggtggta ctgatggagc
     2401 aagattatct tttaatggat taccttgtcc aaatatattt actggtggat taaacttcca
     2461 tgggaaaaat gagtgtatac cagttagttc tatggagaaa gctactaaac taattgtaag
     2521 aatagctgaa aaatatgctg aaagagttta gttaaaaagt atttaaaaat taagcattta
     2581 aagttataat agctatatca ttctttataa gtcaagctga ttataagctt gtgttgtact
     2641 taaaatggta tagctatttt tgtaattatt tttataaaag ataattattt ttataaaaga
     2701 taattatttt tagtttaggg tatcaataaa aagtatgaat atcaataggt attaacattt
     2761 aaaaggggga attgtattga aaattaaatc taaaaaaata atagcatttg cactttcact
     2821 tgggattatg ttgccaagtt gtgtatatgc agacactatt agtaaaaatc aaaatgcaca
     2881 ggtagatatg aacaattatc ttcaaggtag aaaatctcca aaggaggaaa aacttattgg
     2941 tgaagataga tttgatacag cgataaaaat atctcaaagt ggttggaata atggttcaga
     3001 aagggtgttt ctagttaact caaattcatt gccagatgca ttggcttcaa ctcctttggc
     3061 aagtaaattg gatgcgccaa tactgcttac aaataaaaat agtataccat ataatgtaac
     3121 agaggaaata aaaaggctta atccagcaga agttatttta attggaagtg aaggagctat
     3181 ttcatcaagt gtaaaagaat ctcttgaaga tatggaagta ttagtcagta gaatcggtgg
     3241 agcagataga gaagaaacat ctttacttct tactagacag ctagatgata ctggtgattt
     3301 aggtgtctca aaagtggcag tggttaatgg gtataatgga ctggcagatg caacttcaat
     3361 atcaagccca gctgcaagtg ataatacagc tataatatat acaggtaagg atagcataag
     3421 aagtgaggca aagaatttta ttacacaaaa ctcaactaag acatacatag taggtgggga
     3481 gtacagtata agtaaaaaac ttgaaggaca gttagtaaac tcagaaagac tagcagggac
     3541 agatagaaaa gatactaatg cgaaagtttt agaaaagttt tatggaaaat caagtaaggt
     3601 taataatatg tattttgcaa aagatggaag tggtagagaa gctgatttag ttgatgggct
     3661 tgctgctgga gtattagcat caaaaacaaa gtcacctgta gtacttgcaa gtggaagttt
     3721 aagtagtgca cagaaaagtt ttataaaaaa agtaaaggct gataaatttg tacaagttgg
     3781 tggaggtaaa aatagtaaac catatgcaga agcattagcc ttacaataat attgtgtaaa
     3841 tcaaaattaa tatataataa tagcaagtaa accaagttgc acttcaatat atataataaa
     3901 ataagtaaac taagttatat ttcagtataa tatataatga aataagtaaa ctaagttata
     3961 ctccaatata atatataata gaagcaagta aactaagtta tatttcaata tagcatataa
     4021 taaaacaagt aaaccagatt ctatttaatt agaagtttaa ggtattgtac ttaaacttct
     4081 aattgagata gaaataaaat ggtttacttg tttttattat gagtttaata aattaaagct
     4141 tattttattt ttattaaaaa tttactaatc attgatattt attatatttc cagtattgat
     4201 gtcaattaag tttatatcaa gttcaagctc atcctcatca tcagttttta tgatatctat
     4261 aattgcgaca gaatggcttc catcttttgg gatagaagta cttcctggat ttattactat
     4321 taaattatca tccatgtata gtttttttac atgagtatgt cctaaaacta atatgtcacc
     4381 acccattttc tttgctttgt caataatttc ttcttctgat tctatataac catgattcaa
     4441 tataattcta atttcaccaa attggctcat gacataagga ctctgaatag gatgttctat
     4501 aaccatctgg tcaacatctg catcacagtt cccttttaca atcacaatgt tttctaattt
     4561 atttaatgtc tcaataaatt ttttaggatt atatccttct ggaatatcat ttctaggtcc
     4621 atgatataag acatctccac catgtaataa aacatcacaa tcagataata catttaacgc
     4681 tttttcaaag taaagtaagc taccatgagt atcactcatt attcctattt tcatattaaa
     4741 aatcctccta agtacagtct ataaaaattt ttcagatact ctatgccaaa actcatagtt
     4801 tgacattcta agtaatttta tttttgcatc agatatacta acttttatat tttcgatttg
     4861 tctaaatctg tgctcaactc catcaacgac aatcaataca gaatcttcaa atctatattc
     4921 tggtgcaatc tttataacag attcatttga acatattata cttgatgtaa agcacctata
     4981 tgcattggta tttatcggat gtaaaggtgt aatctgcata agttcaagat ttatatcaac
     5041 aatactacct ccagcagaat agttataggc tgttgaacct gtggaagtag aaataatcat
     5101 accatctcca ctaaaatttt gtatatgttt attatcaagg cttaaattta ggtgtatagt
     5161 cctagacttg tctcctctta taacaacctc atttacagca agcatattag aaccactagt
     5221 agtagtatat acttccgcat taagaagtga catttcttga ataatataat ctttttttgt
     5281 ataagcttca ataaagctat caatcttatc tggtaaaata tcaggaaaaa accccagatg
     5341 acctgtattt attcccatta tgggaatctc aggaaaatcg aaatctctaa ctgttcttaa
     5401 aaaagagcca tcaccaccaa ttgatataat aagctctgtg tctggataaa tatctgaata
     5461 cacttcaaag ccagcattga taagttttct agttaagagt tctttagtct caagggactt
     5521 atttaattgg tttgtattta tagttataat tcttttcata tattcacctc ttcatacttt
     5581 gtattataac atagttttat aattcttaat ttataaattt ataaatgacc tttgtaatga
     5641 atgtgttaaa atatgtatag gtgggtataa aaaaatgcat aagaggtgaa gatgtgttta
     5701 gcataaggaa tatcacaaaa aaattaggaa aattcaagtt aaataatata aacttagagt
     5761 taaaggaagg ggatattgtt gggattatag gaccaaatgg ttcgggaaaa acgactttaa
     5821 taaaaattat aatggggatt attgatgcag atgaaggtga aattgaactt tgtaatgaaa
     5881 ctattgaaaa ttctccaata agctttaaaa acaatattgg ttttgtatat gattctttac
     5941 aattttatcc gcatttaaag gttaaggaat ttagaaaaat agtttcttta ttttataaga
     6001 attttgatag agaaaggttt gatgaatacc ttaataagtt tgatatagaa gaaaatatgc
     6061 atattgaaaa tttatctaaa ggtcaatctg aaaagcttat gttatcaagc gcacttagtc
     6121 acaatgctaa gttattgatt ttggatgaac caactgctgg tatagaccca attgtaagaa
     6181 ctgaaataat gcagtattta caagattttg tgaaaaatgg aagtagtagt gtaataattt
     6241 ctactcataa tactgataat ttaattaaaa ttgctgatta tcttgttttt ataaatagag
     6301 gaaatcagat ttttactgtt aagaaagaat taatagaaca agaatataag ataattaggg
     6361 caaataaagc agagttagaa gcaattaaag aaagcatagt tggtgtgaaa gagtataaat
     6421 attataatga ggcacttgtt aaagttggag atagcttgaa agtaaaaagc ctattgatag
     6481 aaattgataa gcataaggtc aaaaatccta ctatagaaga actaatgtat tattatgtaa
     6541 atgaggtgag atgatgttca atttaataag aaaagattta ataataggga ttagttcaga
     6601 tggtatccgt aatttaaagt acatattgct attttttgtg ttttattttt tccttaattc
     6661 aatttcatat tatacagtaa gtattgttgt tagttatttg atatttataa atacgtttga
     6721 gtgtgattat gaaaatgatt caaggatttt tataaggagt atgccagtta gtatagaaga
     6781 tatagtttac tcaaaatatt tattaggtgt aggattaata atttctgtaa caataattgt
     6841 gtccttacta agtaaattga catcactagt gttctttaga aatatggttt taaatgatgt
     6901 atttttttct gtaaatatat ttttagcaat tttgacgatt ttactgcctt taatttttaa
     6961 gtttggatat ggaaaaatga aagtttgtgg ttttatagta agtattttaa tttactttgt
     7021 atatggaagt cttttgaaga tgattagtat gattgtatat caagtaaagc attttaatta
     7081 tagtaaagtt ggaggagtat atctttcaaa ttatgtaact gatatagcta atactaagta
     7141 tataaattta tatagtatga catttttaac aataataatt tttataatat caatgtattt
     7201 ttctattaag atttcaaaaa agaataagtt taattattaa aattttgatt aatattatag
     7261 cacaatagca caaaatataa ggagactaaa aatgaagaag tttataaaag tttcattttt
     7321 tattttgggg atttttacac tattcatagc tataaataaa ttagtatata gagatatggt
     7381 aagtgtcaaa gattatgaaa taaatcattt agaggaaatt gtttttgatt ttgatagcag
     7441 gagaagtaga gatgttggta ctattgtaag atttaaaata agcaataata gtaaatatag
     7501 ttataaatta aacagtgcta aaatgaagtt tgaaaattgt gtggaaaata aaaagggaat
     7561 gaataagtgt tctgcatatg taaatgtaga tttatttaat gaaaaaaatt ctataactga
     7621 aagaactctg aaatatggaa taaaaccaaa taatcaaggt tatgttgatt ttgtaattcc
     7681 aaaaggacta aattttgatt ataggtactt taatgaatct ggtatgagtg tagagtataa
     7741 aggagaatat attgtcaata ttccaatggc aaaaggactt tatctagtag ttggaaaaaa
     7801 taatgataca tggaatgcta atattatgga ataatttgaa aaataaatca attatagata
     7861 taattaagaa taatatgaaa tttataaaag ttcagactag gaggggtatt taatgtcaat
     7921 aagtaatgta agagagtatt ttaaacaatt tggcaaggaa gacagtatat tagaatttga
     7981 acaatctagt gctactgtgg aacttgcagc tgaagcagct ggggttattc cagcaagaat
     8041 agctaagaca ctctcattta aaataggaga tgatgcaatt ttaattgtga cagctggtga
     8101 tgctaagatt gataataaga agtataaggc tgaatttaat tgtaaggcta aaatgttgac
     8161 accagaagaa gtcttggagt ttactggaca tgctattggt ggagtctgtc catttggttt
     8221 aaaaaattct ataaaagtct atttagatga ttcaatgaaa agatttgata cagtatttcc
     8281 tgcatgtggc agtagtaact ctgcaataga acttacatgt gaagaaatgg agaagttttc
     8341 taagtgtgaa aaatgggtag atgtttgtaa gaattggtaa aaaaatatag taaactatag
     8401 taggaattag taaaaaacta cctctgtata caatctatac agaggtagtt tttttgtgta
     8461 aatatttata aatctaagag gaaaatctaa tttaaactaa taatccaaaa taaagttaaa
     8521 aaattctgat tttttttcga aacaataaaa aaatgacaaa aaagtggaaa aaatgtaata
     8581 aatattgtta aaaaaataat tagaaattat tgaaaaatta tcctttgagt gatactatta
     8641 tagatgtaag atatttaaaa ataaaaaata gtcagataaa aatacgatta tttagctcta
     8701 ttaaaataat ttttatacga taaaaatctt ctgtacatgg ctaaaaaaca gatgtacaga
     8761 caatatttaa tgtttaatat accctaatat aaatatcgta aacaaattat tttatctgta
     8821 accagtgtga aagaaatctt attctaactt gaaatttcac aaaattccaa aaagcgagat
     8881 tttttttaag tatagttgat tttaaattaa caatttatga ttaaattaat gtcataattg
     8941 gttagtataa tcacatataa atatagagcg tagtttgatt aaggttataa atttctcgac
     9001 gtatattcaa ataccttatt atagatgttg ccgagatttt aattaataaa tgaaaataaa
     9061 atatttataa aatactgagg aggctattat ggatttaaat tctaaaaaat atcagatgct
     9121 taaagagcta tatgtaagct tcgctgaaaa tgaagttaaa cctttagcaa cagaacttga
     9181 tgaagaagaa agatttcctt atgaaacagt ggaaaaaatg gcaaaagcag gaatgatggg
     9241 tataccatat ccaaaagaat atggtggaga aggtggagac actgtaggat atataatggc
     9301 agttgaagaa ttgtctagag tttgtggtac tacaggagtt atattatcag ctcatacatc
     9361 tcttggctca tggcctatat atcaatatgg taatgaagaa caaaaacaaa aattcttaag
     9421 accactagca agtggagaaa aattaggagc atttggtctt actgagccta atgctggtac
     9481 agatgcgtct ggccaacaaa caactgctgt tttagacggg gatgaataca tacttaatgg
     9541 ctcaaaaata tttataacaa atgcaatagc tggtgacata tatgtagtaa tggcaatgac
     9601 tgataaatct aaggggaaca aaggaatatc agcatttata gttgaaaaag gaactcctgg
     9661 gtttagcttt ggagttaaag aaaagaaaat gggtataaga ggttcagcta cgagtgaatt
     9721 aatatttgag gattgcagaa tacctaaaga aaatttactt ggaaaagaag gtcaaggatt
     9781 taagatagca atgtctactc ttgatggtgg tagaattggt atagctgcac aagctttagg
     9841 tttagcacaa ggtgctcttg atgaaactgt taaatatgta aaagaaagag tacaatttgg
     9901 tagaccatta tcaaaattcc aaaatacaca attccaatta gctgatatgg aagttaaggt
     9961 acaagcggct agacaccttg tatatcaagc agctataaat aaagacttag gaaaacctta
    10021 tggagtagaa gcagcaatgg caaaattatt tgcagctgaa acagctatgg aagttactac
    10081 aaaagctgta caacttcatg gaggatatgg atacactcgt gactatccag tagaaagaat
    10141 gatgagagat gctaagataa ctgaaatata tgaaggaact agtgaagttc aaagaatggt
    10201 tatttcagga aaattattaa aatagataaa tattaaaata tacacaatag gtcgatatta
    10261 aaatagatat ggaggaagga tttatgaata tagtcgtttg tataaaacaa gttccagata
    10321 caacagaagt taaactagat cctaatacag gtactttaat tagagatgga gtaccaagta
    10381 taataaaccc tgatgataaa gcaggtttag aagaagctat aaaattaaaa gaagaaatgg
    10441 gtgctcatgt aactgttata acaatgggac ctcctcaagc agatatggct ttaaaagaag
    10501 ctttagcaat gggtgcagat agaggtatat tattaacaga tagagcattt gcaggtgctg
    10561 atacttgggc aacttcatca gcattagcag gagcattaaa aaatatagat tttgatatta
    10621 taatagctgg aagacaggcg atagatggag atactgcaca agttggacct caaatagctg
    10681 aacatttaaa tcttccatca ataacatatg ctgaagaaat aaaaactgaa ggtgaatatg
    10741 tactagtaaa aagacaattt gaagattgtt gccatgactt aaaagttaaa atgccatgcc
    10801 ttataacaac tcttaaagat atgaacacac caagatacat gaaagttgga agaatatatg
    10861 atgctttcga aaatgatgta gtagaaacat ggactgtaaa agatatagaa gttgaccctt
    10921 ctaatttagg tcttaaaggt tctccaacta gtgtatttaa atcatttaca aaatcagtta
    10981 aaccagctgg tacaatatac aatgaagatg cgaaaacatc agctggaatt atcatagata
    11041 aattaaaaga gaagtatatc atataattta aggggggata aagaatgggt aacgttttag
    11101 tagtaataga acaaagagaa aatgtaattc aaactgtttc tttagaatta ctaggaaagg
    11161 ctacagaaat agcaaaagat tatgatacaa aagtttctgc attactttta ggtagtaagg
    11221 tagaaggttt aatagataca ttagcacact atggtgcaga tgaggtaata gtagtagatg
    11281 atgaagcttt agcagtgtat acaactgaac catatacaaa agcagcttat gaagcaataa
    11341 aagcagctga ccctatagtt gtattatttg gtgcaacttc aataggtaga gatttagcac
    11401 ctagagtttc tgctagaata catacaggtc ttactgctga ctgtacaggt cttgcagtag
    11461 ctgaagatac aaaattatta ttaatgacaa gacctgcctt tggtggaaat ataatggcaa
    11521 caatagtttg taaagatttc agacctcaaa tgtctacagt tagaccaggg gttatgaaga
    11581 aaaatgaacc tgatgaaact aaagaagctg taattaaccg tttcaaggta gaatttaatg
    11641 atgctgataa attagttcaa gttgtacaag taataaaaga agctaaaaaa caagttaaaa
    11701 tagaagatgc taagatatta gtttctgctg gacgtggaat gggtggaaaa gaaaacttag
    11761 acatacttta tgaattagct gaaattatag gtggagaagt ttctggttct cgtgccacta
    11821 tagatgcagg ttggttagat aaagcaagac aagttggtca aactggtaaa actgtaagac
    11881 cagaccttta tatagcatgt ggtatatctg gagcaataca acatatagct ggtatggaag
    11941 atgctgagtt tatagttgct ataaataaaa atccagaagc tccaatattt aaatatgctg
    12001 atgttggtat agttggagat gttcataaag tgcttccaga acttatcagt cagttaagtg
    12061 ttgcaaaaga aaaaggtgaa gttttagcta actaatttct tggggagggt attatgagta
    12121 caagtgatgt taaagtttat gagaatgtag ctgttgaagt agatggaaat atatgtacag
    12181 tgaaaatgaa tagacctaaa gcccttaatg caataaattc aaagacttta gaagaacttt
    12241 atgaagtatt tgtagatatt aataatgatg aaactattga tgttgtaata ttgacagggg
    12301 aaggaaaggc atttgtagct ggagcagata ttgcatacat gaaagattta gatgctgtag
    12361 ctgctaaaga ttttagtatc ttaggagcaa aagcttttgg agaaatagaa aatagtaaaa
    12421 aagtagtgat agctgctgta aacggatttg ctttaggtgg aggatgtgaa cttgcaatgg
    12481 catgtgatat aagaattgca tctgctaaag ctaaatttgg tcagccagaa gtaactcttg
    12541 gaataactcc aggatatgga ggaactcaaa gacttacaag attggttgga atggcaaaag
    12601 caaaagaatt aatctttaca ggtcaagtta taaaagctga tgaagctgaa aaaatagggc
    12661 tagtaaatag agtcgttgag ccagacattt taatagaaga agttgagaaa ttagctaaga
    12721 taatagctaa aaattctcag cttgcagtta gatactctaa agaagcaata caacttggtg
    12781 ctcaaactga tataaatact ggaatagata tagaatctaa tttatttggt ctttgttttt
    12841 caactaaaga ccaaaaagaa ggaatgtcag ctttcgttga aaagagagaa gctaacttta
    12901 taaaagggta aaaatctaat taaaaatata tttgctgtta actagattaa atagtatttt
    12961 aatctagtta acagaatata aatactaaaa taacaggagg ttataactat gaaattagct
    13021 gtaataggta gtggaactat gggaagtggt attgtacaaa cttttgcaag ttgtggacat
    13081 gatgtatgtt taaagagtag aactcaaggt gctatagata aatgtttagc tttattagat
    13141 aaaaatttaa ctaagttagt tactaaggga aaaatggatg aagctacaaa agcagaaata
    13201 ttaagtcatg ttagttcaac tactaactat gaagatttaa aagatatgga tttaataata
    13261 gaagcatctg tagaagacat gaatataaag aaagatgttt tcaagttact agatgaatta
    13321 tgtaaagaag atactatctt ggcaacaaat acttcatcat tatctataac agaaatagct
    13381 tcttctacta agagaccaga taaagttata ggaatgcatt tctttaatcc agttcctatg
    13441 atgaaattag ttgaagttat aagtggtcag ttaacatcaa aagttacttt tgatacagta
    13501 tttgaattat ctaagagtat caataaagta ccagtagatg tatctgaatc tcctggattt
    13561 gtagtaaata gaatacttat acctatgata aatgaagctg ttggtatata tgcagatggt
    13621 gttgcaagta aagaagaaat agatgaagct atgaaattag gagcaaacca tccaatggga
    13681 ccactagcat taggtgattt aatcggatta gatgttgttt tagccataat gaacgtttta
    13741 tatactgagt ttggagatac taaatataga cctcatccac ttttagctaa aatggttaga
    13801 gctaatcaat taggaagaaa aactaagata ggattctatg attataataa ataagaattt
    13861 gggaattaaa aatttaaata aaattgttta aaaaacaatt tcggatatat gaaaaatcta
    13921 atttaatggg ggtaatgaat atgagagaag tagtaattgc cagtgcagct agaacagcag
    13981 taggaagttt tggaggagca tttaaatcag tttcagcggt agagttaggg gtaacagcag
    14041 ctaaagaagc tataaaaaga gctaacataa ctccagatat gatagatgaa tctcttttag
    14101 ggggagtact tacagcaggt cttggacaaa atatagcaag acaaatagca ttaggagcag
    14161 gaataccagt agaaaaacca gctatgacta taaatatagt ttgtggttct ggattaagat
    14221 ctgtttcaat ggcatctcaa cttatagcat taggtgatgc tgatataatg ttagttggtg
    14281 gagctgaaaa catgagtatg tctccttatt tagtaccaag tgcgagatat ggtgcaagaa
    14341 tgggtgatgc tgcttttgtt gattcaatga taaaagatgg attatcagac atatttaata
    14401 actatcacat gggtattact gctgaaaaca tagcagagca atggaatata actagagaag
    14461 aacaagatga attagctctt gcaagtcaaa ataaagctga aaaagctcaa gctgaaggaa
    14521 aatttgatga agaaatagtt cctgttgtta taaaaggaag aaaaggtgac actgtagtag
    14581 ataaagatga atatattaag cctggcacta caatggagaa acttgctaag ttaagacctg
    14641 catttaaaaa agatggaaca gttactgctg gtaatgcatc aggaataaat gatggtgctg
    14701 ctatgctagt agtaatggct aaagaaaaag ctgaagaact aggaatagag cctcttgcaa
    14761 ctatagtttc ttatggaaca gctggtgttg accctaaaat aatgggatat ggaccagttc
    14821 cagcaactaa aaaagcttta gaagctgcta atatgactat tgaagatata gatttagttg
    14881 aagctaatga ggcatttgct gcccaatctg tagctgtaat aagagactta aatatagata
    14941 tgaacaaagt taatgttaat ggtggagcaa tagctatagg acatccaata ggatgctcag
    15001 gagcaagaat acttactaca cttttatatg aaatgaagag aagagatgct aaaactggtc
    15061 ttgctacact ttgtataggt ggaggaatgg gaactacttt aatagttaag agatagtttt
    15121 agattataat atttacaaat taaatattta attatgaatt taacctgtaa gaataactat
    15181 ttgttattct tacaggtttt tatagttaaa aataattatt atctattaaa caaaaatagt
    15241 gtaaaatata taatatcatg attttgaaga tataaaaagt aggaagatat aaaagaaagt
    15301 aggtaaaagt ttgtttaaaa aagagaatca aagatataat cttatatcgt atacaaatga
    15361 agaagaaatg actttaaaag aagttttatt ggacaagcta aatttctcag ttaggtcatt
    15421 atcaaaaatg aagagggaaa agagtgtttt agtaaatgga gtatataaaa aaccgagttt
    15481 aaaggtgtat agtggtgatt taatagaagt aaaaatagac gaggaaaaag caaattttga
    15541 acctcaagat ttaaatttac agataattta tgatgatttt gatataatta tggtgaataa
    15601 acctccgttt atggtagttc acccaacaaa aagccactat gataaaacaa tagcaaatgg
    15661 tattagttac tatatagata atcaaaaaga aaatgtaaaa ataagatttg taaataggtt
    15721 agatatgaat acttcagggt tagttatagt agcaaaaaat gcttatgcac atcatacttt
    15781 gtctacagca atgagtgaaa ataaagttga aaaaaagtat ataacagtag ttgatggaat
    15841 tataaaagaa aatgagggaa ctatagatga gccaatttat agacctacag aagattcaat
    15901 aaagaggata atagatgaaa gaggacagtc atctgttact cactataaag taatagaaag
    15961 actagaaaat gcaactgtat tagaagtaag cttagaaact ggtagaacac atcaaataag
    16021 agtacatatg gctcatatag gtcatggaat aataggtgat gagttgtatg gttatgtaga
    16081 tgaagagcta ataaataggc aagcacttca tgcatataag ttagagtttg aacaaccaag
    16141 aacaaaagaa aaattgaaat ttaaagcaga tataccagag gatatgaaag agttaatatc
    16201 gaaattaaga taatggggtg atatatatga taatacataa atttataata catgttttgg
    16261 acaaaaatag tgatgttcca atattaaatg actttgaagg caaagttaat caagaagtag
    16321 atggtttttt ccagaaagct ataaaaagaa ttgcaaaaga tgaggattta agaaaaggtg
    16381 tttttaaaga ttataatgac aatttaatta aaaactgttg tgaacaaata atatatgatg
    16441 aaagtacttt tttgaaaaat tctaaagaaa ttgcatctta cttgtttgac gttatgaaaa
    16501 ttaatgctgc actagaatct tgtgatttgg caatttgttt atacactatt aaagatgaaa
    16561 agagtgtagc aatactgaaa ttagattata aaaagttgta cactcattca attgaatttg
    16621 tggatgataa atttaatatt caaatggttt caaatgaaat tggtatccca gagactttaa
    16681 gacaaaagca aggagctttg attagtctaa gtggtataaa tgatgagttt catctaaggc
    16741 ttttagataa ggatgctgaa aaagaagggt cagaatctaa gtttgtaaca gaatttttaa
    16801 atgctaaaaa aatagatgat gataagtaca agaccaaggt atttaaaaat acagctgaaa
    16861 attggataac caatgctctt agtaatgata taaagcaagc tgaagatgta agaagtatat
    16921 taaattatac actgaaggaa aaacatgaag ttgatataaa tgactttgtt gataattcaa
    16981 ttaaagatga cgagttaaag gatagcttta aagagcatat ggaagaaaaa ggtcttgatg
    17041 aaagttttag tatagataaa aaatgggttg agaaaaagct taaaaaaaga agtataaaaa
    17101 cggataatgg ttttgacata aaagggaatc taactgattt tgaagaccca atgaagtata
    17161 ctgtaaaaca aaatcaagat ggaactatag atataataat aaagaatgtt acgttttatg
    17221 aagaaaagta atttatatac ttagtatata aattaaatat aagagcgtgt attttatata
    17281 ttgtaaataa atgtaatcta gtctgtgcga actctaatat aatttattat gtagaagttt
    17341 acacagacta tttatatttg tagacaaaac gatactattt gttttcttcg atatatttac
    17401 atatatcacc aatacattta aagttctcag cttcctcgtc tggtatttct attccaaatt
    17461 catcttcaag ggccattata atttcaacag catctaatga atcagcttct aagtcgtcca
    17521 ttaaagatgc ttccatagtt atctcatcta aattgtctac ccctaattgt tctgctatta
    17581 tttcttttat tttctcaaac attttaagcc tcctaaagca gttttaatta attttaatgt
    17641 tagtttaata taatatcaag aatttttcaa tatggaatct ataaaaaata ataaaatttt
    17701 ataaatgtag taataaagta ttatttgatg aataaacttt attgataaca aatataactt
    17761 aggaggaaat gctatgaaat atgtaggact attactaact tcagtaggaa tgtttttact
    17821 tatagcagta aatttttatt ataattcaat aactttagat atgcaacgta tagaggatta
    17881 tgtaatggag accaatttaa tccttgaaga tgtagctgaa aaggagagtt atgtatctaa
    17941 tgaaaaagaa gactacatat caaggttaat gcatgtaaag aaaggaatag aaaattcaaa
    18001 aacatccttc ttgatagaaa gatataagga gtacaaaata aaatcaatag aaagtttaat
    18061 ctatactata tcagaggaga aaaaagatta tctggatgaa gttgatagat acaataaact
    18121 tggtgaaaaa gaaataaata aactaatcaa taaaaacttt ttagaagtaa cttatctgtc
    18181 tattactaca tatatttaat taaagagaca aaacttagtc tcttaatgat tcaaggagga
    18241 ttagttatga caaaattaga agacaatatg gaacgtttct ttggaggatg cgaaggattt
    18301 tttgggaata aaatgttgat aataatagtt atagtattct tactattatg cacagacatt
    18361 ttagaagact tattatgcga tgacaatata tggatatggg ttatattaat agtattgtta
    18421 ttatttaact tcgatgatgg ttgttgttgc taaaaaatat tgattattaa ataggtaaaa
    18481 ccagtaactt atacaagtta tggttttacc ttttttagtt caaattaaaa aatttaaaaa
    18541 aatagtaaaa atacattagc aaaatggaaa ccaaatgcat agtatatgtt aggagcgaaa
    18601 agctcaacaa aatatcaaca ggaggtaacg tataatgtta gatagattct ttggagaaga
    18661 aggatgtttt ggtggaggag gatggtggat aatagtacta ttcttcctat tcttagcttt
    18721 tggagatagc tgggcagaat ttgacataat ggcatggata ccattcctaa tagttttatt
    18781 aatactttgc tgctgtggtg gatgcttaga ataagaacac agttattaaa aaaaggaggg
    18841 ctttgttatg ttagatagat tttttggaga agaaggatgc tttggaggag gaggatggtg
    18901 gataatagta ctattcttcc tattcttagc ttttgaagat tgctggagag acattgacat
    18961 aatgtcatgg atacctttct taatattatt gctaataact tgttcatgtg gtggattctt
    19021 tgatggtgaa gtaggttgcg gctgctaata cttaaatcat tagaataaaa aaatgctggt
    19081 attttatacc agcatttttt tattttattt tttaatatga tttaaattaa gaatgaatat
    19141 tattaaattt gttgaatata gggtataata taaaatataa tgtatttaaa tgtacttgct
    19201 tttatggtaa aataaaaact tatataaatg tagttaaaat aggaaccttc aaaaatttgg
    19261 ttcagtttta gataaattta gtaaaataag gaggatgaag atgaaaggca atataacgat
    19321 aaaagatgtt gctaaacaag caggagtgtc aatatctact gtatctagag ttataaatga
    19381 ttcaaaacct gtaactgatg aagtcaaaca aaaagtttta gaggttataa aagagactgg
    19441 atatatacca aatccacttg ctagaagctt agtaacaaag aagagtcaat taataggggt
    19501 aatagttcca gaagtttcag attcttttgt taatgaggtg ttaaatggga tagaagaggt
    19561 tgctaaaatg tatgactatg atattctttt agcgaataca tactctgata aggaacaaga
    19621 acttaagagt ataaatctat tgagagcaaa acaagtggaa ggtatagtta tgatttcatg
    19681 gatagttgaa caagaacata tcaactatat acaaaattgt ggaataccag cgacatatat
    19741 aagtaaaact gctagaaatt atgatatata tacagtaagt actagcaacg aagaagctac
    19801 ttttgatatg acagagcatc ttataaagaa aggtcatgaa aagatagctt ttataatgac
    19861 gagtaaagat gatactgttt tagaaatgga aagacttgct ggttatgaga aagcactttc
    19921 aaataacaat atagaattag acaagagttt gattaagtat ggtggaactg attatgagag
    19981 tggatacaat agtatgaaag aactattaga tgatggaata atacctcatg cggcttttgt
    20041 aacaggtgat gaggctgcca taggtgctat aaatgctata tgtgatgctg gatataaggt
    20101 tccagaagac atatctgttg caggatttaa tgatgttaag atagctagaa tgtatagacc
    20161 taaacttact acagtatatc aacctctata cgatatggga gcagtagcaa taagaatggt
    20221 tataaaatta ataaataagg aattaattga aaataagaaa atagaattac cttatagaat
    20281 tgttgataga gaaagtgtta cagaaagaaa aaaataaaac tattaaaatc aatcaggtag
    20341 tgcctaaggc actacctgat tttttatgtc aataatatca aatttaataa tagattaact
    20401 aaaaataatt atctaattac ttaaatagtt tatttttatc tattcatgag ggttagactt
    20461 tttagttttc ttaaataaac ttttagattt tttttgttga ctacctggtg tattttttgg
    20521 tgcaggtgaa tttgacttac ctttagtagg tttagccttc ttagttttag ctggtttagt
    20581 atctctttgg ttttctgcat ctgagatttt ttccattata ttatccagtt ttttgcgttg
    20641 attttcatct aaaaatgctt tgaattttga tactacttca tctctgccat ctagattctt
    20701 acctattttt ataagttcct ccataagttc attttctgat tttcctttgt aactatcagc
    20761 catcttttct attttatcct tgtcaatatt tttttgttta gccatttttt ctaatgactc
    20821 tgtatctaat tttttcataa aaaaacccct ttctataaat atatattgaa ttatattcat
    20881 actatttata tatagaaata atattgaagt ggtgaattgt gagtgatata taatataaat
    20941 aaaaattctt atagataata tatatgcact gagctttaaa aatggaacat taaaatatga
    21001 aaaacatact ttaatatagg gcaattatat aatataaggg ggaattgtct tgaataactt
    21061 actatttttg attggagtga ttgcattgag taatggtaat atctctatat ttgatgataa
    21121 aaataaatca aaaggaaata atagagttag aaaaaacaaa aaaaatgcaa atgataaact
    21181 ttcacagtct acacatggca atttaaggtt tgatgcaaat gatataaaaa gaggtttaag
    21241 gctcatggat ttaagtgaag aagatttaga aatgggaatg gaaataataa caagaactaa
    21301 aaaatacatg tcaagagatg aaagaaaaat actaataaaa atagaaagtg ttttagattt
    21361 agttagagga ataaaaaaat taaataatat agatatggta gatacagaag atgaaacgga
    21421 ttttttcaga aaaatggata gtgatgataa aaagaatatg atgataaagg aaattataga
    21481 tgtattccca gaaaaaagaa aaaattcagt tgaaaaagct ataggaatga aaaagaagat
    21541 agatttattt gcagaattat ttttaccaga tgactttggt gaaggtggat ttagtttgag
    21601 ctctttggca aatataaaca atcttggtag tatgaataat ctcaaattgt taggaagtct
    21661 tttaagaggt gatgatagta ataatgatga tgaagacaat aatgatgagt taaattatga
    21721 agacgaagaa ttagattgtg aagacaaaga attggatgat gatgagttaa cagatgaagg
    21781 tgaaatggag gataatgaag atgaattagt agaagataag aacacaggct atgaaaatat
    21841 agaaaatgat gaagatatag tgtacgatga ggaactatac aacattaatg agcaagaatc
    21901 cgaatacaat tataatgaaa aaaatactaa aactagagct aagaaaaaac gataattaac
    21961 taaatacata aagaaaaaag gaagaaaaag taatgataga ataagattat ctcaattact
    22021 atataaaacc actttaaaaa attcaaaatt tattatataa taataatatg aaatcctgaa
    22081 acatacgata aggctttttt aaattcaacc tacaaatgaa atacgggaga ccgtgtctgg
    22141 taaattataa catgcctagt tagtttggac gttaaaggtt actggcaagt cacccacctg
    22201 ggagtgccta gggtgtgaat ttacttagcc accggtgttt cgggacaaaa aaagtgctac
    22261 tatttcaaat agtagtactt tttttattaa tatgcatttt tctactaaat acgcctaatt
    22321 attgataaat tcaaaaagca agtaacaatt aagattataa aagcatagta ttaattaggc
    22381 ttcaaaaagc caaagactta ataagaatcg cttacaggga gggcaatgaa aaatgcaaga
    22441 ttataaaaaa aataaaagaa gaatgatgaa tcagccaatg tctacaatga atgaagaaga
    22501 agtgtataca gatgaaataa attcagaaga catgagaggt tttaaaaaat cacaccatca
    22561 taatggatgt aatactgata ataagtgtga gtgccatgat gattgcaatc catgcaaccc
    22621 atgtaatcca tgtaaaccta acccatgcaa tccatgcaaa cctaatccat gtgatgacaa
    22681 ttgtggatgc catgacaatt gtaaatgtga ttgtgaacca tgtgaaatgg attcagatga
    22741 atgttttgaa aacaaatgtg gaccagaatg ctgtaatcct atatctccaa gaaacttctc
    22801 tgtatcaaat gcagtgccat ttgcaataga ggctaataga atatttgata ctatgcaatt
    22861 ccaaacattt acagatgcaa caggaccaaa tggagagcca ttaacttttg aaacagaagt
    22921 agtagaagta tttggttcag ttccaagtgc aggtcaagca agtgtaacta tagaaaaaat
    22981 atgcttaagt aatgatggaa tcgttataga cacaggaatg acaactttag aagatttcga
    23041 tttagaccca ttaggagata tagtaggaag aaactgtgaa acaacttttg aatttgcagt
    23101 ttgtggagaa agaaactctg agtgctgtag acaaggaaaa ggcaaatcag tagcttataa
    23161 acaaagagga ttaactgtag cagttcgtaa tttagtacta gagctaagag gtagatgtgg
    23221 atgtacagag ttcgttgcat tagctttccc agcagttaga gcaggaggtg gatgtaagag
    23281 aagagttgat tatgtagaat ttacttttaa cacactttca gcaccaatat gcttgccagc
    23341 tgacggaaga gctgttactt taagacaaga atatcaaact aacttaactg tagattgtat
    23401 aggaaaatct atattaaaat tagaatgcaa cgaatgttgt gaacctttct atgaattaat
    23461 tataccaaat gatatagatt tagtactttg cttacaagaa acagttagca cattaataag
    23521 tgaacaaata gtagttttag catcaccaaa tccaatccaa ccaagacttg ttgatacttt
    23581 ctctaaagta tgtgattttt cgcaatgtgg acctaatcat ggaagtggaa agccaagttg
    23641 ccacagatag ataggtagat atatagacag tttaaattat aaataaaaat catctaagga
    23701 aggctaccaa agtgtataat ttacattggt agccttcacg tttttataac tagcgtatag
    23761 attttagtga tttaaaaagc actatagtat gtaatagaca agtaaagatt gatactatcg
    23821 tcattgtgta tatgtatgca taaatattaa gtcctggaat tggaagaaat acatataaag
    23881 ctattagttg aattaacatg ccagtaatag tatttatact tgaagctaat tcttttctta
    23941 ttgaatgtaa aatgccagat agagtttgat ttagggacat aaatagtgga actaaaaaca
    24001 tagcttttag atattctcct gcaagtgtat ttttaaatac taatatacat aaatcttttc
    24061 caaagaaata aaaaaatatt gatgatagta tgcctacaaa taaagtaaga agtatggagt
    24121 atttgatttt tttaattaca ctcttacgct ttcttagagc catttcctgt gaaatagttg
    24181 gtattaaatt tacaataagc gctgaaccaa cagtaaaagg taaaaatata aaaggcatta
    24241 ccatcccact gacaatacca tacatactta aagattgttg ataagtcatt cctgataatg
    24301 ctaatcttga tggtatcatc atagaactta tagattgcaa aagtatgttt gacattctat
    24361 tacaagttat gggaatagac atttttaatg tttccataga agaattataa aaatctttta
    24421 tgcttataat atacctattg tcaaatgatg aatctcgcca taagcaaata agcataaata
    24481 taatattact caactcacca atcgatatac ctaatagagc tatataacaa ttcatagatt
    24541 tattatttat gtacataaca agtaaaaaaa caaataatat tttaccaatt tgttcaatga
    24601 tttgtccaat agcagggatt tttacattct ttatgccata gtaatatcct cttagtacgt
    24661 ttgaaagagt tattataact atagcaggac aaacagctag tataaataga tttaatttag
    24721 catcttttaa aaatttaagt gataaaaaag aactattgaa agatgcaact atagatataa
    24781 ttagtgctac gaaaaatgat atatataaag ttgatataaa taaagcatta gtattgtgtc
    24841 tatcgtttaa agcttttctc tttgcaacta gacaactaag tgcagtaggt atacctgttg
    24901 ttgtaacagc taagcaaatc atcaaaaagt taaaaatcat atgatagata cctaaacctg
    24961 tttcacctag tgctcttgat aagaatattt tatataaaaa accaaatatt cttacaataa
    25021 aattggatat aaataaaata actgttgata gtactaaata aggtactttc aaaaaaatca
    25081 cctccttatc ttttaaatat attcacaagg aggtgtaaaa atttataatt ttataaattc
    25141 tggtttcaat ttattaaatt tagatacata tttaaatccc atatttttta gtaatgaata
    25201 tatgtagtca aactcacatg caacttgact tatatgatgt gaatctgaac ctgtggttat
    25261 gatttcccct cccaagtctt tatacatttg aagtatatac ttagatgggg ttaggtctgg
    25321 aagattatat ctataacaag aggtatttat ctcgatacct ttgccatcat aaatagcttg
    25381 tttaagagtt tcttcaatta tatcagaaaa tagtctatca tctaaaatag tgtcataagg
    25441 tgcatacctt ttaattaaat ctaaatgacc taagacagag taatctttat aattcttaac
    25501 aatattatat aaataaagat agtaattttc ataaacttca tgttgagttt tatctttaaa
    25561 ataattacca aggtataagt ccattgtatc tatagcatgg atagaacata ttataaagtc
    25621 caatggatat tgatgtgctt cctttttgta tgtatccatt aattgagttt gaactccaaa
    25681 ttcaattcct ttttttattg atattttatc tttatatttg ctttgaagag tctctaaact
    25741 cttaaaataa tcttcataga caattgaaaa agaatcatca gcatatacat catagtcaac
    25801 atggtctgtg aaacaaattt cctccaaacc gagttcaata gattttttta ccatttgttc
    25861 cattgtagat tttccgtctg tagagaaact tgagtgcatg tgatagtcat aaaacataaa
    25921 tatttcccct ttttataaaa attttggtat tatatataca tactaaataa tatcaaatat
    25981 acataaaagc atcaataaaa tataaataca atgagcggtg ataaatttgg aaaaatacag
    26041 aatagattta aatgagaaaa aatcaactaa atatatacag atatttaatc atataaaaga
    26101 attgataata aataaagagt tagtagaaca tgaaaaattg cctccaataa gaaaactatc
    26161 aaatttttta caagtaaata acactactat agttaaggta tacgagcttt tagaaaatga
    26221 aggatatgta tataaaatta tagggagtgg tacttttgta tcaagtttaa attcttataa
    26281 aagcggtata gataacagga aaaataagat tattgtgcaa agagaagatt taattcattt
    26341 tgacaatggg aatccatcta atgatatgtt tcctatcgat tcatttaaaa atgctgtaaa
    26401 tatggcatta tctaaagatg gtagttcaat atttgaatat gatgaaggtc ttggctctga
    26461 agaattaaga gaaaaaatgg tagagtatct aagtgatgga aatataaaaa ctactaagga
    26521 aaatattcaa ataatatcag gtgctcaaca aggtatagat atagtttgta aaggacttat
    26581 aagttattca gatgttgttt ttatggaaga accttcttac aatggagcaa ttgaagtgtt
    26641 taaaagtaga ggtgcaaaaa taatatctat acctatgtta gatgatggaa ttgatatagg
    26701 tattttaaaa ttaaagttgg aaaaaataaa accaagactt atatacatta tgcctaattt
    26761 tcaaaatcca actggaatat cttactcaac atataaaaaa aagaaattga tagaattggc
    26821 agaagaacat gatttttata taatagaaga tgattttata agtgattttg tttttgaatc
    26881 aaaggacaat agaacaatta gaagttatga tgataaaaat agagtagtat atataaaaag
    26941 cttttcaaaa atacttatgc caggtcttag gataggaatt gtagaaatgc caaatgagtt
    27001 gttaaaaaaa gttttatggg ctaaatattc atcagatata tcaacaccag gtttaattca
    27061 aaaatcaatg tattactata tgaataattt tgattggaag aattatttga gtcatataga
    27121 gaagatatat actgataagt ataaattggc aaaaaacttg attaatgaca aattgagtgg
    27181 taaattaaaa gttagaaaat cttgtggagg aataaacttt tttttagaat taccaagagg
    27241 atatacatct caagcattta caaattttat gttaaacaaa ggagtgtcaa tgcttccagg
    27301 gacttatttt tttgacaatc tagtggatga tagattcttt aggataaata tagctcgtcc
    27361 aagcatggaa gaactagaaa agggaatatc tataataagt gataatatag atgagttttt
    27421 ttgtgaatat aaaaacaagt tagatataaa aagcaataaa ttattttatt aaataaatag
    27481 aggtttttta ggaggaaaaa atgtttgaca agaagaaatt agataggatt aatgaattag
    27541 caaagaaaaa taaagaggga atcctttctg ctgatgaaat caaagaaaga gaaattttaa
    27601 ggaaagaata tttagaaaac tttagagccc actttagatc aagactagat agtgtcaaag
    27661 tagtttcacc agaagaatat gagcaataca tgaaaaacaa taaaaattag aacttagtag
    27721 gaggaaaata tgaacttaaa gtacgaattt aagaatgctt gggattttga aagaaaagaa
    27781 aatactattg aacaaattat gcaatattct cgcaattata tggagttttt aagtaagtca
    27841 aagactgaaa gactttcagt aaaagaaatt ataaaattgg ctaaagaaaa taactatatt
    27901 tcaatagatg aagctatgga aaaaggaagc ataagttgtg gtgataaaat ttatgttata
    27961 aataaagaga aggcagtagc actttttgta ataggtaaaa attatataga aaaaggtatg
    28021 aagattatag gaagccatat agattcgcca agattagatt taaaaccaaa tccactgtat
    28081 caagagagta atctaggttt ttttaagact cattattatg gtggaataaa aaaataccaa
    28141 tggacggcta ttccacttgc tttacatggt atagttatat taaatgatgg aacaaaagtg
    28201 gacatatcta ttggggaaga agatagtgac ccagtatttt gtgtaactga tttattgatt
    28261 catttggctg gagaccaaat gcaaaagaaa ctatcagaag gaattagtgg agaagcttta
    28321 aatatattaa ttgggaatat tcctttagaa gatgaagaaa aagaacctat aacagcaaat
    28381 atattaaaga ttttaaatga gaaatacaat atagttgaag aagatttatt aagtgctgaa
    28441 attgaagtag ttccagctgg taaggctaga gatttagggc ttgataggtc tatggtgttg
    28501 ggatatgggc atgatgatag ggtatgttca tatgctgctg taaaagctat actagaaact
    28561 gaacaaccag aatttacttc agttgcatta tgtgtagata aagaagaaat tggttctaaa
    28621 gggaatacag gtatgcactc aaagtttttt gaaaatactg tggctgaact gattgcttta
    28681 gaaggagatt attgtgatat taaagtaaga agagctttag caaatagtaa ggtattatca
    28741 gctgatgttt ctgctggata tgacccaaat tttggagaag catatgaaaa gagaaattcg
    28801 gcatatatgg gaaatggagt agttttaact aagtatacag gttcaagagg aaagagtgga
    28861 tgtaatgatg ctaatgctga atttatgtca gaagtaaaga gaatttttaa taaaggaaat
    28921 gtagtatggc aaactgctga acttggaaaa gtagaccaag gaggaggcgg aactatagcc
    28981 catatattgg caaatcaagg tgcagaggtt atagactgtg gtgttggagt tttaaatatg
    29041 catgcaccac atgaaattgt ctctaaagta gacatatatg aaatgtataa gggatataag
    29101 gcatttttta atataaattt ataagtaaaa atataaatgt aataagtgaa taaaggctgt
    29161 tgtataaaca atttaatctg ttgtacaaca gtcttatttt tatgtaaaaa tagcaatttg
    29221 tcacactaga gaaaataaaa gaaaatactg tcaaattaaa aattatttaa aaaaaaattg
    29281 acaacgattt catcttgtta taatatgtaa taaagaactt gttatataat gttataaaaa
    29341 tgttacatag ggaggtgagc aaaatggatg aaaagagaca aaaaaaagaa atggatttag
    29401 aagtaaattc ctttgttcct ttatatcaac aactatatga caacataaaa aaacaaatag
    29461 catctggcat atataaacca ggagataaac ttccatctga aggagactta tgtaaagaat
    29521 ttaatataag tcgtataact gtgagaaatg cacttaatga acttgtaaaa gaagatatat
    29581 tatgtaaaaa acgtggtaaa gggacttatg taacaatacc agagagaata gaagcaacat
    29641 gtgcaggaaa tagttttact aattcttgtc atcgtattaa tgcaaaacca agtaccaaaa
    29701 taatctctgt tttaataaaa aaagcagata agcaagtagc tgaagcttta agtattgaac
    29761 tagaagagaa ggtaatatgt attaaaagac ttagattaat agatgaagta cctgttattt
    29821 ttgaagttga ctattttaga atagattaca tgtttttatt aaaagaagaa ttagaagata
    29881 aatctttaat ggaggttata tctaataata taagcacatt acctaaacga gtagaaaata
    29941 tatttgaagt aaaacattca aacaaagaat attctgacca tcttaaatgt gcaagtaata
    30001 tgccactatt gaaagtaaga caatcggtgt acacagaaaa taatgatgtc ttatattaca
    30061 atgagcaatt tataagaagt gataagtaca aatatgcagt atcagcagaa atataatcta
    30121 taatttagta ggaggatgag tatgaaaaag ataatattag catctcatgg agacttttca
    30181 aaagggcttt taaatgcagt aaaaatgata gtaggagatt tagcagattg tgtcagttcg
    30241 tatggtttgt accctggaca aagtgcttca gattttgctt tagaattaga aaaaacaata
    30301 tcagaagata aagaatgtga atatataatt ttatctgact tatatggagc aagtgtatgt
    30361 acagcaatgt taagattaac taatctacat aatgttaggt tattttctgg aatgaatttg
    30421 aatatggtat tagaacttct tacaaggttt tctgatgaat taacagttga agatatagac
    30481 caattagtgg aagaatctag aagaggaata caaagcgtga cattacaaat taaagaagaa
    30541 gaagaggtat tttagtttat gaaaaaaaag ggaatattat cagtatttaa aagtaaaaaa
    30601 ccaattatag caatgataca cttaaaagga gatactccag aagatatctt tgaaagagct
    30661 aaaaaagaga ttacaatctt tgaagaaaat ggtgtagatg gaataatgtt agaaaattac
    30721 tatggtaatt attatgattt agaaagaata cttgaatatg tatcaaaagc taatttatca
    30781 ataccatatg gagtaaactg tttaaatgtt gatactatgg gatttgaact ggcaacaaaa
    30841 tataatgcta gttatataca ggttgattct gtagtaggtc atgttaaacc tagagatgaa
    30901 gctactttag aagagttttt taaattacaa cgttcaaaat gcccagcgta tttaattgga
    30961 ggcgtacgtt ttaaatatca gccagtatta tcagaaaatg atgttgaaga agatttaaaa
    31021 ataggaatga caagatgtga tgctatagca gttacagaaa atgctacagg tcaagaaaca
    31081 tcaatggaaa aaatagaact gtttcgtaaa aatttaggag acttcccatt ggttatagct
    31141 gctggagtta cattggaaaa tgcaaaaaaa cagcttaaat taggagatat ggcaataata
    31201 ggaagttact ttaaagataa ttacaaagat tttggggatg ttagtgtaga acacgtaaaa
    31261 acattcatgg atgaaatcaa aaaaattagg gaggaattat aatgattaaa ttagtaagag
    31321 tagaccacag acttatacat ggacaagtag catttacatg gacaaagttt ttaagtacag
    31381 actgtatatt gatagcaagt gatgacttat taaaagatga attgagaatg gcaggactta
    31441 aaatggctaa accatctaat gttaagttag taatgaaaag catagcagat tctataaaag
    31501 cacttaactc aggtgttact gataaataca acttattgat actttgtgaa tctgtagaag
    31561 atgtttatag acttgctaaa gaggtaaaag ctattaaatc tataaacctt ggtggaacaa
    31621 aatcagatga taatcgtgaa aatatatcta aagcagtgca tgtatcaaaa gatgatataa
    31681 agatgattaa agagttagat tcagaaggcg taaatgtatt tgtacaatta gttcctgatg
    31741 atgatgctac aaatgtaatg aaattaatat aattttaggg ggatgaaaat ggaatttaca
    31801 caagttattt tgataactct aattgcattc tttgcatata tgcatagttt tgtaggttct
    31861 acaatgcata atagacctat agttgttgca ccacttgttg gtttagcact aggtaatctt
    31921 catacaggta tagtaatagg ttcaacatta gaattagtat ttatgggtgc atttcctgtt
    31981 ggggcaagta atcctcctga ttttgtgtct ggttctatta ttgccacagc gtttgtaata
    32041 ttaacgggtc aagatgtatc agcagcagtt gtacttgcag ttcctatagc aactctagta
    32101 ttgcttatag ataacttctt aatgacagta gtacttacat ggggagctca tattgctgac
    32161 cgttatgcag aagaggggaa tattgaaggc gtagaacgtg tgcaattatt atttggtata
    32221 ggaaataaat taatattagc aataatagta ggtataggat tttcattagg tgttccagta
    32281 atagaaaaaa tactttcatt tataccatca tatgtaactc atggaatgga tgttgcagca
    32341 ggcgtgattc cagcaatagg ttttgccatg ttagctagaa tgatgcttaa taagaaaaca
    32401 gtagcatttt tattattagg atttatattg gttgcttacc taaacataac agtaacagga
    32461 gttgcattgt ttggattagc aatagcttta atatatgtta actttgtagg agagaaggag
    32521 gtaatagtag atgacaacga gttctaagaa attagagaca atttcaccag atagtaaaat
    32581 aacgaggaaa gacttttgga aatgttttag aaggtcattg acattagatt catcttggaa
    32641 ctacgaacgt atgcaaaata tagcatatgc atacatgatg gcaccaataa tccgtagatt
    32701 atataaagat gataaagaaa agaaatctaa ggctttaaaa agacatttag aatttatgtc
    32761 agttactcct catataagta ctcttttggt aggtatatct ggagctatgg aagaagaaaa
    32821 tgcaaagaat aaagaatttg atgctaatag tataaatgct gttaaatcaa gtttaatggg
    32881 tccagtttct gggataggag attcattttt ctggggaaca ttaaagctta tagcagctgg
    32941 ggttggtata gctttagctt ctcagggtaa cataatggga ccgattttat tccttttaat
    33001 aataaatgtt cctcatttta ttatacgcta tatatgtcta gataaaggat ttaagtatgg
    33061 aactcaattc tttaaagatg tgagtggttc aagtatagtt tcaaaagtta tggaagcagc
    33121 ttcaatgtta gggcttatgg taattggagg tatgactgca tctaatgtaa tgttaaaact
    33181 tagtgttaat gtaggtagtg gagaatgggc agagcctatt caaacttact tagaccaaat
    33241 aatgccatgt atgcttccag ctatgatatt cggtattatg tattggttat taggaaaaaa
    33301 ggtaaagaca actacgatat taatttcagt tatgattatt tgtatagtat tggctgctat
    33361 tggagtagta taaataaaaa aatcccaagt tcatttgaac gagggatttt ttgactaaat
    33421 taaataaaaa agaaagatgg ttcataataa tttataagca tttatgtgat agttataatt
    33481 agcttattaa ttagttataa actcaaaatt aagcactact ttattaaagt tattatatta
    33541 taatttcgaa atactcacat aatctgttta gaaatttatc ttctaatggg gaaaaagttt
    33601 tctttttaga gtaaatgaag aaaaactttc tagtaaaatc aacatccttt attttataac
    33661 attttatttt acctgcattg agataatcga tagctgaaat gtacgatata aaggatactc
    33721 caagacctaa tctaaccatt tccttaatag cctcatttga ttctacatga gcgattacat
    33781 ttaatttact tactggaaag ttatttttac taagtgtgtt tagtattaag tttcttgtac
    33841 ctgacccttc ttttctcata ataaaattta gataagccaa ttctcctata tcgatacaat
    33901 tatttttatt atcaatttta aaatctgaag gagtgataag aacaagttca tcatctaata
    33961 agtctaggta ctctatttga ggattattga ttttagaacc aactaatcca aagcttattc
    34021 gttcatttaa aatttctgat atagcatatt gagaatcata atggcttatg gaaaatttta
    34081 catctggata actcatagaa aaactcttca taaaatcagg taaaatataa gtttcaggta
    34141 tggaactaca tgctatatct atgatacctt ctatttttcc tgaatattct tttatatcat
    34201 aaattgcacg tttgcaatta ttcaatatat atatagcatg ttcataaaga atttcaccag
    34261 attttgtgag tgtaataact ttgttgcttc tatttataag aacagtttct agctctctct
    34321 ctaaattctg tatgtgtgcg cttacagtag gttgagttaa aaaaagttct cttgcagcct
    34381 ttgaaaaact ctgatgtttt gcaacagcaa caaatacttc aagttgttta aaatccatta
    34441 tttaatcctc cttaaattat ctaaaactat tataacatat ttgataaatt aatagtcaaa
    34501 ggtgattttt aaaatagaaa acctttaaat attcatggag tttttgattg tttaggaata
    34561 atttggtata atgttgtaag gtacttacaa taaaaatatc gtaaattgaa ttttcaaaat
    34621 tcaactttaa agtgtttttt attacagaaa aattacaatt agcttcttaa tcttattaaa
    34681 ttcaaataaa tattatataa ttattttata atataataaa tataattatt gaaaaaagag
    34741 ataactgtaa attgagatat agataaatac tataaaattt aagaaaggag gattgattta
    34801 tgaagttaaa aaggtcatta gtttgtgtaa ctattttagg tattatactt gttggatgtc
    34861 ataaagaaaa tactaaagag aaaaatcaag tagcaagtaa agcaacacag caaaaaacta
    34921 tgacaaaagt tcaaaatgat gttaatgaaa ttatgaataa agattataag tacattataa
    34981 agaatatggg tataccttat aatacatttt attatataaa acctaaagtt ttaaaagaat
    35041 caaatacaat gcaagatata aatacatcaa gttatatgac attagtttat ccaaaatata
    35101 ctggaaatga tgaactagat ggaagtgctt tatatgttga tattaacgaa aataaagttg
    35161 tcaatgttga aaccaattct ttctcatcac aaggtatatc agtcattgat gctgaatcta
    35221 gtatagtgat tgaaaaaagt gaccatgaaa aatcggctgt atcactagaa aattttagac
    35281 atattgacct gggagagtat gttggtgttg aagattccag aataaatgaa attgtaggag
    35341 atgcgaatta tgatttaaca gcatataatc atgaaggcag taaagtagtt aagagttata
    35401 gattgaaaga agataacaaa atattaaaaa aagaggtact tacaataagt attgtggata
    35461 acaaaataaa atctataaaa actattgaaa gtgataaaat tgtaaaaata ataaaaggaa
    35521 ctttattaga ataaaatatt ctaaagcata tgatgctgtt tcaagataga aggaaatttc
    35581 tagctgggaa cagttttttt attagattta taaaatttga actgcactta tagataacta
    35641 ttagttataa ttaaatataa gataaaagta tttccaactt ttataatcta taatatatta
    35701 catagaaata tcttaaataa aaaatatatt ggggagtatt taaaatgata gaattagaag
    35761 atgccataaa aattattgaa aaaaatgttt taccaataaa cagggtcaag atagtaaatc
    35821 ttatagatgc agtaaataag gtagttgcag aagatatata ttcaacaatt gatagtcctc
    35881 catttgatag gtcaccctat gatggttatg catataatgc aaattctgaa aatagaaatt
    35941 taaaggttat agggactttg tatgcaggag atgtatttaa tagggtatta aataaaaatg
    36001 aagcagtgaa gataatgact ggtggaaaga taccacaggg tgcaaattgt gtaataaaaa
    36061 aagaagatgt aacagttata ggtgatatga taactttaaa tataaaatta aaggaatacg
    36121 ataattacat atttaagggt gaggatataa aaaaggggca attgattatt aataaaaatc
    36181 agaatatagg atatgatggt attggagtat tagcaagtct tggaatatcc aaagtaactg
    36241 tttatgatga tttaaaagtt ggaattttga atacaggtac agaactacaa gatataaatg
    36301 agactttaga gaatggtaaa atatacgata gtaatagata taccttatat tcaagattat
    36361 taaaacttgg aatacaagca gatacaatat gcaattgttc tgatgatata ttggatttag
    36421 aaaaaaaggt aaagaatatg ttacagagtg tagatttttt aattaccaca ggtggtgttt
    36481 ctgttggaga taaagattta attccagatg tattcaaaaa aataggggca acagaattat
    36541 tttggaagat aaacattaaa cctggtggag cttgttatgt aggtacattg ggagaaaagc
    36601 tattatttgg tctttctggt aatccacatg cagcaatagt ggtatttgat aatgtgatag
    36661 ttcctgttat tgagtattta acatataaga agggaacaca tcatataaaa gctatattta
    36721 aaggagaatt taataaactt tcaaataaag atagatttgt aagtggaaaa ctatatacaa
    36781 gtgaaggtaa attgtatgta gagctaaatg ataagaaaga tgcaaaagct agactatctg
    36841 caactctaag aagtaattgt atgattaaga ttaaaaagaa tgagacaata aaagaaaatc
    36901 aattagtgga tgttgttatt agatagtttt atgaaaggac acaattttat gtataatata
    36961 ttgtcagttg tctcatattc aggagtaggt aaaactacgt tgattgaagg cataataaaa
    37021 gaattaaata aaaaagggta tcgtgttgga gttattaaac atacctgtca tgattttgat
    37081 atggatgagg aaggaaaaga tacttataaa catagaaaat ctggggcgag aaaagtctgt
    37141 ataatatcag ataaacgagt agcatatatt gaagaactaa aagagaagaa tccattatat
    37201 aaaatgattc aattatatga agatatggac ttgattatca tagaaggata taaaaattat
    37261 agatttaaaa gattggaagt aactagaaaa ggtaagtatg aggatatttt atctaataag
    37321 tctgatttga taggtattgt cagtgatata gaatataaat tgaatattga acagtttaat
    37381 ttaaatgatt ataaaaatat atcaaagtac atagagtctt gtataaaaaa tgaaatttta
    37441 agtataagta atgttgaaat aaaaaacata ttaaaagaat aattttaaga tgttcttctt
    37501 atataaaatg atataattat ttatataaat aaagatataa gaaatttatc aattttattt
    37561 tgagttataa aatattaaag aaattgtagg aggacaatat atgaaaaaaa tattagtagc
    37621 ctgtggagca ggaatcgcaa catcaacaat agtttgtgac agagtagaaa gattggttaa
    37681 agaaaataat atacaagcag aagttgagca atgtaaaatt gctgaatgtt caacaaaaca
    37741 agaaggtgct gatttaatcg tatctactac tatactacca acaacttatg atatacctac
    37801 aataaaggca acaggatata taacaggtat aaatacaaca gcattagata aaaagatatt
    37861 aaatgcattg aagtagtttt gtatttataa aaaaaattta gtattaaata tagagttgta
    37921 tctttataaa attttaaata tagagatata gctcttttta tgttgtttca agtatacaac
    37981 tatatgatat aaatcttcaa ttttatgaaa aagattgtta ttactttgta agtttataaa
    38041 agatttaaag ttattgttat gttatactaa tttatgggta ttttttagaa gtactaatag
    38101 aagtactaat aaaggaggga tttaatactt atgataaaac aatttgtcaa atattataag
    38161 ccatataaga aaatttttac actagacctt atagcagctt ttctattttc cttatgtgat
    38221 ttagtttatc ctatgataac tagaaatata atggatgacg ttgttccaaa taaaaactta
    38281 agaatgttag tagtttttgc agtagcactt atacttatat ttatagcaaa agcagggctt
    38341 aactatttta tgcagtattg gggacatgtt attggtgtag atatgcaagc tgatatgaga
    38401 aatgaggtat ttacacactt acaaagactt ccaaatacgt attttgataa taacaaatct
    38461 ggagtaacta tgtctagaat agtaaatgat ttaatggata taacagaact tgcacatcat
    38521 gggcctgaag atttattcat ttctatagta atgttggttg gttcattctt tatacttata
    38581 gatataaata ttcctcttac gcttataata tttgctatat taccgtttat aatttggttt
    38641 gcaatagcta aaaaagataa gatgaatatt gcttttatga agagtagagt tactataggt
    38701 gatgtaaatg ctactttaga aaatagtata gcaggtatga aggtaactaa gtctttttgt
    38761 actgagaaag aagagttaaa taagtttgta agaagcaata aattatttag aagagcaaga
    38821 caagattctt ataaggtaat ggcagaatat tactctggta tgaatttata catggatatc
    38881 ttagagtggg tagttgtaat agctggtggt tattttactt atataggaaa gattactttg
    38941 ggtgattttg ctgcttatat actatatgta aaaatgttta tacaacctat gaaaaaatta
    39001 ataaacttta cagagcaata tcaaaatggt atgacaggtt ttaaaagatt catagagata
    39061 atggaacaag accatcaaaa agaagctaaa aatcctattg aacttgaaaa tgtaaaaggt
    39121 gacattgaga tagaaaatat atctttcaca tatgaagata agactcaagt tttggataat
    39181 ttaagtttat ctataaaagc tggtaagact atagcattag ttggaccttc tggaggagga
    39241 aagactacat tatgtaatct attgccaaga ttttatgaat ttgataaggg tgatataaaa
    39301 attgatggaa agagtataaa agatgttagt ttaaaatcat tgagaaaaaa tataggtata
    39361 gttcaacaag atgtattttt atttacagga actataagag ataatattct ttgtgggaac
    39421 cctaatgcta cagatgaaga aatgatagct gctgctaaaa aagctagaat acatgacttt
    39481 gtagagactt tgccagatgg atatgatact tatataggag aaagaggcgt aaaattatca
    39541 ggtgggcaaa aacaaagaat ttcaatttca agaatctttt taaagaatcc tccaataata
    39601 atattggatg aggctacatc tgctttagac aatgtaacag aaagagaaat tcaagagtct
    39661 ttagaggaat taagtaaaga tagaacaaat ttagttgttg cacatagatt gactactata
    39721 aaaaatgctg atgaaatcat agtattgaca gataaaggaa ttgaagaaag aggtactcat
    39781 gaagagctag taaacaaaaa tggagtatat agtaggttac acaataacta gtatacaaat
    39841 tttacaccat agctaacata cacattaaac ttacaaacat aggatatatt aagattcata
    39901 aagacaattc attttatatt tataagatgt tttatgaata aaatgtatcc tatgttttat
    39961 tttttgcaaa ttacatttaa ttctctttgt aagaatagag ttaaatagcc aatggtcggg
    40021 ataacgtttt tacaaaaaga ataatttaga aaactaactt taatttatgg aatttatcga
    40081 attttatgaa aaaaatcgaa ttttgacttg aaatgttttt caaaagtagt aaaataatgg
    40141 ataactatac atacatttta taaaaatata tttattatga ggggggattt tgtatggagg
    40201 caataacagc tttttgtcta gttttagtat cactgacgat aggagatata gtttcagcaa
    40261 aaactaaagc ttttgttccg tcggtttttg tatcagcaat tatatttata gtaggatttt
    40321 ggactttttt cccagaaaat attgtggact tagccgcttt aggaacacca ttagctcaat
    40381 taggtatgct tttgttaata actcatatgg gaactatgat gagtattaag gaattagcag
    40441 gtcaatggaa aacaatagta atagccttgg caggaatagt aggaatatgt gtaggtgctt
    40501 tggcattagg gacagtagta tttggatggg atacagcagt aatagctaca cctcctctta
    40561 ctggagggct agttgcatct ataatgatgg cagatgctgc tacagcaaaa ggtcttgcaa
    40621 gtttatcagt acttgcaatt ttgatgtatg ttgcacaagg atttgcgggt tatcctatta
    40681 cagcattaat gcttaaaaaa gaaggaaaaa gacttttatc agactttaga agtggaaaag
    40741 taactgtaaa tacagatgaa gaaaaagtta aggatttacc tgaacaaaaa agcagattta
    40801 agataattcc agacttacca gaaaaatatg atacgacata tatgataata cttagattag
    40861 gatttgtagc atggttagca gtacaattta caaatcttac aaatgaagtt gtatcaagat
    40921 atgttatatg tttaatattt ggtgtaatag catcagaact tggaatagtt gatagaaaac
    40981 cacttaataa atctggttca tttggatggt taatgacatc attgatggct tttatatttg
    41041 cagggttagc aaaagctaca ccagcaatgc ttgttgaaat ggcagttcct cttgtaggaa
    41101 tcataatatt tggtgtaata ggtatgggaa tattctctat tatagtaggt aagcttttag
    41161 gtgaaagtaa agaaatggct ttttcagttt cgcttacatc actttatggt tttccaccaa
    41221 actatatatt aactgaagag gcagtaaagg ctttggctga aacaccagaa gaaaaagaat
    41281 ttttaatgga taagatgctt ccaaagatgc ttgttggagg atttactaca gttacaatag
    41341 cttctgtagt aatagcgggt atctttgtaa atctaatata gtatttgagt gataaaaagt
    41401 attttaaata agaagaatgt gtactacttg gaggtatgga atggatatta aagaaataac
    41461 aaagagttat aaagactatg taattaagct tagaagggaa tttcatgaaa atcctgagaa
    41521 aagtatggaa gaagttagaa catcaaagag agttaaagaa gaacttgata aaatagggat
    41581 accatatgta tcagctggag gtacaggtgt aattgcaact ataaaaggag ctaatccagg
    41641 aaagactgta gcacttaggg gtgatatgga tgctctacaa gttgttgaat gcactgatgt
    41701 agaatacaaa tctaaaaatg aaggacttat gcatgcttgt ggtcatgatg gtcacacatc
    41761 aatgcttcta ggggcagcaa aagtgttaaa tgatattaag gatagtatca atggaactgt
    41821 aaaattattt tttcagccag gagaagaagt tggtaaaggt gcaagagcta tgattcaaga
    41881 tggtgctatg gaaggtgtag atagtgtgtt tggaatacat ctttggactg atgtagaatc
    41941 tgggaccata tcggtagaag aaggaccaag aatggcttct gctgatttct ttaaaattac
    42001 tgtaaaaggt agaggtggtc atggttcact tcctcatcaa ggtgttgatg cagtgcttgc
    42061 aagttcagca atagttatga atctacaatc tatggttagt agagaagtaa gtcctctgga
    42121 accattagtt gtgagtgttg gagttttaaa ttctggaact agatttaatg taattgcgag
    42181 tgaagctatc ttagaaggta caataaggct atttaatcca gagcttagaa agcaaattcc
    42241 tggaatttta gaacgtatag caaaatctac agcagaagca tatagagcag atgctgaatt
    42301 agagtatggg tacttaacac cagcagttat aaatgacaaa gaatgttcaa agatagctac
    42361 agatgcagca attaagctat ttggagaaga ctgtataaca ctatttgaaa aagtaacagg
    42421 agcagaagat ttagctgagt ttatgaatat agcacctggt gcactagcat ttgtaggagc
    42481 tagaaatgag tcaaaaggtg cttgttatcc tcatcatcat ggatgcttta atatagatga
    42541 agatgctcta gaaattggaa ctgctttata tgtgcaatat gcagtggatt tcttaaataa
    42601 ataattttaa tatactataa agaaaaagca gagactaagt ttatatctct gcttttttat
    42661 tgtgtatatt ttttataaat attgactata taaaatatat atgatagcat aaagaaaatg
    42721 ataatcaata tcaattaaaa agaaggtgat ttatttgaat aatgtaatat ttgcattttt
    42781 gataacatta ttggctggtt tatctactgg tataggaagt tgtatagcat tttttgctaa
    42841 aaaaacaaat actaagtttc tttctataag cttaggattt tctgctggtg ttatgattta
    42901 tgtttctatg atagaaattt ttcctaaagc acaagatgct ttgacaaaat ctatgggaga
    42961 gaaattagga agttggtcaa ctgtaatagc tttttttata ggtatggcta tcattgctgt
    43021 tattgataag ctaattccac aagaagaaaa tccacatgaa ataaagaaaa tggagaatat
    43081 agaagaaaag aatatcaaaa aaaataagtc tcttttaaga acaggtatat ttacagcaat
    43141 ggctatagca attcacaatt ttcctgaagg attagctaca tttatatcag ctcttgatga
    43201 tgttacaata gctattccga tagcaattgc aattgcaata cacaatatac cagaaggtat
    43261 atctgtatct gtaccagttt attatgccac aggagataaa aagaaagctt tttattattc
    43321 ctttttatct ggtatgtctg agcctttagg agcgattatt ggatatgtac ttcttagaaa
    43381 ttttttaaat gatataactc ttggaattgt atttgcaatt gtaggaggaa ttatggtatt
    43441 catatcacta gatgaattat tgccatcagc tagagagtac ggggaacatc atttatctat
    43501 ttatggattg attgcaggaa tgggtgttat ggctattagt ttattactat ttaaataact
    43561 tttaaatata tgattgtaac ctaaaattag tatcaaaatg atataattat ttaaatagat
    43621 ttttaagcta tttaaaaata tcattttgga gaatgagtgc tatggaaaag aaaataaaat
    43681 tcaaatttga ctggtatagt tcacctgata agatagagaa atggttagaa gatattgaag
    43741 ctaaaggata taatttatat aagataaata aatttggagg aatattttat tttcataagg
    43801 gaagtccaag aaaaataaaa tacactgttg attactacta tgatgtagag aataattatt
    43861 ttgatgaaca taaatcaaat ggctggaatc ttgtatttag gagtattggt agttttatgg
    43921 gtacatctgc atggtat
//
LOCUS       DAGSHT010000013        41402 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGSHT010000013 DAGSHT010000000
VERSION     DAGSHT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.014"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="MJB72_000649"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="MJB72_000649"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..365
                     /locus_tag="MJB72_000650"
     tRNA            291..365
                     /locus_tag="MJB72_000650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
     gene            372..457
                     /locus_tag="MJB72_000651"
     tRNA            372..457
                     /locus_tag="MJB72_000651"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:406..408,aa:Leu,seq:taa)
     gene            473..548
                     /locus_tag="MJB72_000652"
     tRNA            473..548
                     /locus_tag="MJB72_000652"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:506..508,aa:Met,seq:cat)
     gene            556..630
                     /locus_tag="MJB72_000653"
     tRNA            556..630
                     /locus_tag="MJB72_000653"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:589..591,aa:Glu,seq:ttc)
     gene            640..713
                     /locus_tag="MJB72_000654"
     tRNA            640..713
                     /locus_tag="MJB72_000654"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:672..674,aa:Gly,seq:tcc)
     gene            719..794
                     /locus_tag="MJB72_000655"
     tRNA            719..794
                     /locus_tag="MJB72_000655"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:752..754,aa:Val,seq:tac)
     gene            800..876
                     /locus_tag="MJB72_000656"
     tRNA            800..876
                     /locus_tag="MJB72_000656"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Asp,seq:gtc)
     gene            886..960
                     /locus_tag="MJB72_000657"
     tRNA            886..960
                     /locus_tag="MJB72_000657"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:918..920,aa:Thr,seq:tgt)
     gene            975..1059
                     /locus_tag="MJB72_000658"
     tRNA            975..1059
                     /locus_tag="MJB72_000658"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1009..1011,aa:Tyr,seq:gta)
     gene            1069..1152
                     /locus_tag="MJB72_000659"
     tRNA            1069..1152
                     /locus_tag="MJB72_000659"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1103..1105,aa:Leu,seq:tag)
     gene            1182..1258
                     /locus_tag="MJB72_000660"
     tRNA            1182..1258
                     /locus_tag="MJB72_000660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Arg,seq:tct)
     gene            1266..1341
                     /locus_tag="MJB72_000661"
     tRNA            1266..1341
                     /locus_tag="MJB72_000661"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1299..1301,aa:Gln,seq:ttg)
     gene            1430..1518
                     /locus_tag="MJB72_000662"
     tRNA            1430..1518
                     /locus_tag="MJB72_000662"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1464..1466,aa:Ser,seq:tga)
     gene            1522..1597
                     /locus_tag="MJB72_000663"
     tRNA            1522..1597
                     /locus_tag="MJB72_000663"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Phe,seq:gaa)
     gene            1604..1677
                     /locus_tag="MJB72_000664"
     tRNA            1604..1677
                     /locus_tag="MJB72_000664"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Met,seq:cat)
     gene            1692..1768
                     /locus_tag="MJB72_000665"
     tRNA            1692..1768
                     /locus_tag="MJB72_000665"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1726..1728,aa:Ile,seq:cat)
     gene            1792..1868
                     /locus_tag="MJB72_000666"
     tRNA            1792..1868
                     /locus_tag="MJB72_000666"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Pro,seq:tgg)
     gene            1876..1952
                     /locus_tag="MJB72_000667"
     tRNA            1876..1952
                     /locus_tag="MJB72_000667"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1910..1912,aa:His,seq:gtg)
     gene            1961..2036
                     /locus_tag="MJB72_000668"
     tRNA            1961..2036
                     /locus_tag="MJB72_000668"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1994..1996,aa:Lys,seq:ttt)
     gene            2044..2117
                     /locus_tag="MJB72_000669"
     tRNA            2044..2117
                     /locus_tag="MJB72_000669"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2076..2078,aa:Cys,seq:gca)
     gene            2124..2198
                     /locus_tag="MJB72_000670"
     tRNA            2124..2198
                     /locus_tag="MJB72_000670"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2156..2158,aa:Asn,seq:gtt)
     gene            2204..2289
                     /locus_tag="MJB72_000671"
     tRNA            2204..2289
                     /locus_tag="MJB72_000671"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2238..2240,aa:Leu,seq:taa)
     gene            2305..2380
                     /locus_tag="MJB72_000672"
     tRNA            2305..2380
                     /locus_tag="MJB72_000672"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2338..2340,aa:Met,seq:cat)
     gene            2388..2462
                     /locus_tag="MJB72_000673"
     tRNA            2388..2462
                     /locus_tag="MJB72_000673"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2421..2423,aa:Glu,seq:ttc)
     gene            2472..2545
                     /locus_tag="MJB72_000674"
     tRNA            2472..2545
                     /locus_tag="MJB72_000674"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2504..2506,aa:Gly,seq:tcc)
     gene            2551..2626
                     /locus_tag="MJB72_000675"
     tRNA            2551..2626
                     /locus_tag="MJB72_000675"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2584..2586,aa:Val,seq:tac)
     gene            2632..2708
                     /locus_tag="MJB72_000676"
     tRNA            2632..2708
                     /locus_tag="MJB72_000676"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2666..2668,aa:Asp,seq:gtc)
     gene            2718..2792
                     /locus_tag="MJB72_000677"
     tRNA            2718..2792
                     /locus_tag="MJB72_000677"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2750..2752,aa:Thr,seq:tgt)
     gene            2807..2891
                     /locus_tag="MJB72_000678"
     tRNA            2807..2891
                     /locus_tag="MJB72_000678"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2841..2843,aa:Tyr,seq:gta)
     gene            2901..2984
                     /locus_tag="MJB72_000679"
     tRNA            2901..2984
                     /locus_tag="MJB72_000679"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2935..2937,aa:Leu,seq:tag)
     gene            3008..3082
                     /locus_tag="MJB72_000680"
     tRNA            3008..3082
                     /locus_tag="MJB72_000680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3040..3042,aa:Gly,seq:gcc)
     gene            3107..3183
                     /locus_tag="MJB72_000681"
     tRNA            3107..3183
                     /locus_tag="MJB72_000681"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3141..3143,aa:Arg,seq:tct)
     gene            3193..3268
                     /locus_tag="MJB72_000682"
     tRNA            3193..3268
                     /locus_tag="MJB72_000682"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3226..3228,aa:Gln,seq:ttg)
     gene            3277..3352
                     /locus_tag="MJB72_000683"
     tRNA            3277..3352
                     /locus_tag="MJB72_000683"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3310..3312,aa:Lys,seq:ttt)
     gene            3355..3443
                     /locus_tag="MJB72_000684"
     tRNA            3355..3443
                     /locus_tag="MJB72_000684"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3389..3391,aa:Ser,seq:tga)
     gene            3447..3522
                     /locus_tag="MJB72_000685"
     tRNA            3447..3522
                     /locus_tag="MJB72_000685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3480..3482,aa:Phe,seq:gaa)
     gene            3529..3602
                     /locus_tag="MJB72_000686"
     tRNA            3529..3602
                     /locus_tag="MJB72_000686"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3563..3565,aa:Met,seq:cat)
     gene            3617..3693
                     /locus_tag="MJB72_000687"
     tRNA            3617..3693
                     /locus_tag="MJB72_000687"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3651..3653,aa:Ile,seq:cat)
     gene            3711..3787
                     /locus_tag="MJB72_000688"
     tRNA            3711..3787
                     /locus_tag="MJB72_000688"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3745..3747,aa:His,seq:gtg)
     gene            3796..3871
                     /locus_tag="MJB72_000689"
     tRNA            3796..3871
                     /locus_tag="MJB72_000689"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3829..3831,aa:Lys,seq:ttt)
     gene            3879..3952
                     /locus_tag="MJB72_000690"
     tRNA            3879..3952
                     /locus_tag="MJB72_000690"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3911..3913,aa:Cys,seq:gca)
     gene            3960..4036
                     /locus_tag="MJB72_000691"
     tRNA            3960..4036
                     /locus_tag="MJB72_000691"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3994..3996,aa:Arg,seq:acg)
     gene            4049..4124
                     /locus_tag="MJB72_000692"
     tRNA            4049..4124
                     /locus_tag="MJB72_000692"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4082..4084,aa:Val,seq:tac)
     gene            4200..4613
                     /locus_tag="MJB72_000693"
     CDS             4200..4613
                     /locus_tag="MJB72_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261819.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            4922..8353
                     /locus_tag="MJB72_000694"
     CDS             4922..8353
                     /locus_tag="MJB72_000694"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBY8261820.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            8595..10523
                     /locus_tag="MJB72_000695"
     CDS             8595..10523
                     /locus_tag="MJB72_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBY8261821.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            10976..11440
                     /locus_tag="MJB72_000696"
     CDS             10976..11440
                     /locus_tag="MJB72_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBY8261822.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            11447..11941
                     /locus_tag="MJB72_000697"
     CDS             11447..11941
                     /locus_tag="MJB72_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBY8261823.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            11957..12982
                     /locus_tag="MJB72_000698"
     CDS             11957..12982
                     /locus_tag="MJB72_000698"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBY8261824.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            12969..15416
                     /locus_tag="MJB72_000699"
     CDS             12969..15416
                     /locus_tag="MJB72_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBY8261825.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            15596..16969
                     /gene="radA"
                     /locus_tag="MJB72_000700"
     CDS             15596..16969
                     /gene="radA"
                     /locus_tag="MJB72_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBY8261826.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            16972..18042
                     /gene="disA"
                     /locus_tag="MJB72_000701"
     CDS             16972..18042
                     /gene="disA"
                     /locus_tag="MJB72_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBY8261827.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            18131..19225
                     /locus_tag="MJB72_000702"
     CDS             18131..19225
                     /locus_tag="MJB72_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBY8261828.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            19354..21531
                     /locus_tag="MJB72_000703"
     CDS             19354..21531
                     /locus_tag="MJB72_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261829.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATASIYVIVPMITLSINLTALKE"
     gene            complement(21717..22562)
                     /locus_tag="MJB72_000704"
     CDS             complement(21717..22562)
                     /locus_tag="MJB72_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8261830.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            22779..23945
                     /locus_tag="MJB72_000705"
     CDS             22779..23945
                     /locus_tag="MJB72_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBY8261831.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLEKLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            23967..25613
                     /locus_tag="MJB72_000706"
     CDS             23967..25613
                     /locus_tag="MJB72_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBY8261832.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            25710..27083
                     /locus_tag="MJB72_000707"
     CDS             25710..27083
                     /locus_tag="MJB72_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8261833.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            27528..28481
                     /locus_tag="MJB72_000708"
     CDS             27528..28481
                     /locus_tag="MJB72_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBY8261834.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            28519..29481
                     /locus_tag="MJB72_000709"
     CDS             28519..29481
                     /locus_tag="MJB72_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBY8261835.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            29506..30492
                     /locus_tag="MJB72_000710"
     CDS             29506..30492
                     /locus_tag="MJB72_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBY8261836.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEVAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            30505..31551
                     /locus_tag="MJB72_000711"
     CDS             30505..31551
                     /locus_tag="MJB72_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBY8261837.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETILGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            31718..33448
                     /gene="lpdA"
                     /locus_tag="MJB72_000712"
     CDS             31718..33448
                     /gene="lpdA"
                     /locus_tag="MJB72_000712"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBY8261838.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASIGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            33866..36814
                     /locus_tag="MJB72_000713"
     CDS             33866..36814
                     /locus_tag="MJB72_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261839.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEKIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNAKADFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            36792..37256
                     /locus_tag="MJB72_000714"
     CDS             36792..37256
                     /locus_tag="MJB72_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8261840.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            37369..37671
                     /locus_tag="MJB72_000715"
     CDS             37369..37671
                     /locus_tag="MJB72_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8261841.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            37733..39097
                     /locus_tag="MJB72_000716"
     CDS             37733..39097
                     /locus_tag="MJB72_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY8261842.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            39132..39605
                     /locus_tag="MJB72_000717"
     CDS             39132..39605
                     /locus_tag="MJB72_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261843.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            39777..40427
                     /locus_tag="MJB72_000718"
     CDS             39777..40427
                     /locus_tag="MJB72_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBY8261844.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECSSTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            40601..>41402
                     /locus_tag="MJB72_000719"
     CDS             40601..>41402
                     /locus_tag="MJB72_000719"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBY8261845.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKF"
BASE COUNT        16297 a         4863 c         7988 g        12254 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agccaaaaaa agccgaagtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggga
      421 cttacctctc gtaccggttc gattccggtc ttcggcactt ataaaacaat atcgcggagt
      481 ggagcagttg gcagctcgtc gggctcataa cccgaaggtc gtaggttcaa gtcctgcctc
      541 cgcaaccatt aaaatggctc gttggtcaag aggttaagac accgcccttt cacggcggta
      601 acaggggttc aattccccta cgagtcacca attaaatatg cgggtgtagc tcaatggtag
      661 agttctggcc ttccaagcca gctgtgaggg ttcgatcccc ttcacccgct ccaaaaatgg
      721 gaccatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag gttcgagccc
      781 tgttggtccc accatttgcg gcctggtagt tcagttggtt agaatgccag cctgtcacgc
      841 tggaggtcga gggttcgaac cccttccagg tcgccaaaat aaaatgctgg tgtggctcaa
      901 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      961 tgtaaaatta atacggagga tttcccgagc ggccaaaggg ggcagactgt aaatctgttg
     1021 tcatcgactt cggtggttcg aatccaccat cctccaccaa aaaaatgcgc ggatgtggcg
     1081 gaattggcag acgcactaga cttaggatct agcgccattg gcgtgggggt tcgactccct
     1141 tcatccgcac catttttgtt tgatatacat atataaattg tgggtgcata gctcagctgg
     1201 atagagcaac gcccttctaa ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca
     1261 tatattgggg attagccaag tcggtaaggc acatgacttt gactcatgta tgcgtaggtt
     1321 cgagtcctgc atccccagcc aaatgatgta ttactaagct atatttgaat tatttttatt
     1381 ttaaatatag ttaggttcaa ttttagaata agtttaagta tccgaatgtg gagaggtgtc
     1441 cgagtggttt aaggagctgg tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga
     1501 atcccaccct ctccgccaaa tgcccagata gctcagtcgg tagagcaggg gactgaaaat
     1561 ccccgtgtcg gtggttcgat tccgcctctg ggcaccaaaa tatggcggta tagcttagtt
     1621 ggctagagcg ttcggttcat acccgaaagg tcataggttc gactcctatt accgctacta
     1681 aaatttaatg tgggccatta gctcagttgg ttagagcgcc cggctcataa ccggtaggtc
     1741 tggggttcga gtccctgatg gcccaccaca ttaaattaat taatataaat tcgaggtgta
     1801 gcgcagtttg gtagcgcaca tggtttggga ccatggggcc gggggttcga gtcccttcac
     1861 ctcgaccaat ataaggtggg tatagctcag ttggttagag cgccagattg tggctctgga
     1921 ggtcgtgagt tcgactctca ttatccaccc caattcatat ggtctattag ctcagtcggt
     1981 agagcacctg acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa
     2041 ggtggcgaca tagccaagtg gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa
     2101 tctgggtgtc gcctccaaaa tattccaagg tagctcaacg gtggagcaac cggctgttaa
     2161 ccggtaggct gtgggttcga gccccaccct tggagccaaa aaagccgaag tggcggaact
     2221 ggcagacgca caggacttaa aatcctgcgg gacttacctc tcgtaccggt tcgattccgg
     2281 tcttcggcac ttataaaaca atatcgcgga gtggagcagt tggcagctcg tcgggctcat
     2341 aacccgaagg tcgtaggttc aagtcctgcc tccgcaacca ttaaaatggc tcgttggtca
     2401 agaggttaag acaccgccct ttcacggcgg taacaggggt tcaattcccc tacgagtcac
     2461 caattaaata tgcgggtgta gctcaatggt agagttctgg ccttccaagc cagctgtgag
     2521 ggttcgatcc ccttcacccg ctccaaaaat gggaccatag ctcagctggg agagcacctg
     2581 ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg cggcctggta
     2641 gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga gccccttcca
     2701 ggtcgccaaa ataaaatgct ggtgtggctc aacggtagag cagctgactt gtaatcagca
     2761 ggttgtaggt tcgattccta tcaccagctc catataaaat taatacggag gatttcccga
     2821 gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt cgaatccacc
     2881 atcctccacc aaaaaaatgc gcggatgtgg cggaattggc agacgcacta gacttaggat
     2941 ctagcgccat tggcgtgggg gttcgactcc cttcatccgc accattttta tttaatttaa
     3001 taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga agtcgcgagt
     3061 tcgaatctcg tttcccgctc caaattttat gtttatgttg taatgtgggt gcatagctca
     3121 gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc tgtgcgctca
     3181 ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac tcatgtatgc
     3241 gtaggttcga gtcctgcatc cccagccaaa cagtatggtc tattagctca gtcggtagag
     3301 cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caatggagag
     3361 gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag gggccgtggg
     3421 ttcgaatccc accctctccg ccaaatgccc agatagctca gtcggtagag caggggactg
     3481 aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caaaatgtgg cggtatagct
     3541 tagttggcta gagcgttcgg ttcatacccg aaaggtcata ggttcgactc ctattaccgc
     3601 tactgaaatt taatgtgggc cattagctca gttggttaga gcgcccggct cataaccggt
     3661 aggtctgggg ttcgagtccc tgatggccca ccacattaaa ttaacttatg gtgggtatag
     3721 ctcagttggt tagagcgcca gattgtggct ctggaggtcg tgagttcgac tctcattatc
     3781 caccccaact tatatggtct attagctcag tcggtagagc acctgacttt taatcagggt
     3841 gtcccgcgtt cgagtcgcgg atagatcacc aaaaaggcgg cgacatagcc aagtggtaag
     3901 gcagtggact gcaactcctt gatccccagt tcgaatctgg gtgtcgcctc caataaaatg
     3961 cgctcatagc tcaactggat agagtgtctg actacgaatc agaaggttag gggttcgagt
     4021 ccctttgggc gcaccatatg aatagcgagg gattatagct cagctgggag agcacctgcc
     4081 ttacaagcag ggggtcacag gttcgagccc tgttaatccc accagtgcaa acctctagta
     4141 aaactactag aggttttatt tttgattgaa aaagttttta tatttaggag gtaaaaaatt
     4201 tgtatatagt aataggaaat gaaatagttg atagtgaaga acttaaaata attatagata
     4261 aaaatagtga atttaaagtt gaaaaagatt taagtaaaag cacaaaaaga gaagatgtaa
     4321 tagcatatca gttaagtata gatttaaatt acctagattc tcttataaat gagcaatgta
     4381 atttagccag tctatctgat gaagagaaat ttgatgaata tatgactttg tctgatgaac
     4441 ttgcactgga tttagaagag ttaatgccaa aatatacaat aataaatgct agagcatata
     4501 aattagacga ggtagatgga attgtaaaaa taatactagc tgtagcgtat gcagatttag
     4561 ggcacttgaa actttctgat gtagtaaaga ggctatctag acaagtagat taggatatga
     4621 atatgaacta tattaaaaaa atgtataaaa tcttacgtaa agatgaaaat aattatgtaa
     4681 acatagaaaa aaacagtata aaatcaagtc aattgtagaa attttcacat aaattcatct
     4741 gaaattctat aaaaaatgcc ttaattttaa tgaggtaaat cgagtttaca cagtctaaaa
     4801 attacttata tttataaatt tatcttatta attaaggaac tttcttaaac atcttcaaaa
     4861 ataaagtatt ttagtctata atctttaata ttacacaaac aataaaggag aagaatagag
     4921 tatgcttaaa aagtttaata aaatactagt ggctaataga ggcgaaatag ctataagaat
     4981 atttagagcc tgttcagaat tggggataaa aagtgttggt atatacagta aggaagataa
     5041 gtatggattg tttagaacaa aagcggatga atcatatttg ataggagaag gtaaagggcc
     5101 aatagatgca tatttagata tggatggaat aatagaccta gcaaagagaa aaaaagttga
     5161 tgctatacat ccaggatatg gttttctagc ggagaatgca gaatttgctc gtaaatgtga
     5221 agagaatgga attactttta taggaccatc atctaaggta atgaatatga tgggggataa
     5281 gattaactct aaaaaaatcg caaaagaagt taatgttcaa actataccag gagtagaaaa
     5341 agcaataaga agtactgaag aagcaaagga agttgcaaac aaaataggat atcctgttat
     5401 gataaaagca tctaatggcg gtggcggtag aggtatgaga attgtacatc gcgaagaaga
     5461 cttagagctt gaatatgaaa ctgcttgtag tgaatcaaga aaagcttttg gagaagatat
     5521 tatatttata gaaaaatata tagctgatcc aaaacatata gaagttcaaa tattaggtga
     5581 taattatgga aatatagttc acttatatga aagagattgt tcagttcaaa gaagacatca
     5641 aaaaataata gaatatgcac cagctttttc attagatgat aaagtaagaa aagagatatg
     5701 tgaagatgca gttaagttat caaaacatgt gggttattct aatgcgggaa cattagaatt
     5761 tttagtagat gcaaatggtg ggcattattt tatagagatg aacacaagag tacaggttga
     5821 acatactgta acagagatgg ttacaggaat agatatagtt caaagtcaaa tactaatagc
     5881 ccaaggttac agcttagata gtgaagaaat taatataaaa tcacaagatg atgtggaaat
     5941 aagaggttac tctatccaat gtagaataac tacagaagac cctaagaata aattcatgcc
     6001 tgatacaggt aaaattcaag tttatagaac tggttcagga tttggtataa gactagatgg
     6061 aggaaatgga tttacagggg caaacataag tcctcattat gatagtttat tggttaagac
     6121 aatatcttgg gatagaacat tccaaggagc aataaataaa actataagat ctataaaaga
     6181 acttagagtt agaggtgtta aaactaatgt aggattccta gttaatgttt taaataatcc
     6241 tatattctca aatggtaaat gtagtactaa gtttatagat gaaaatccag atttatttga
     6301 aataacagaa agtaaagaca gaggaacaaa attacttcaa tttataggag atgtaattgt
     6361 aaatgataat gcttgtaaag aaaaaccatt atttgatgca ttacatgacc caagaatgga
     6421 taaggatggt tcaaaatcag aaggaagtaa aatacttttc gataagttag gtaaatctgc
     6481 atatatagaa aagataaaaa atgataagaa attactatta acagatacaa caatgagaga
     6541 tgcacatcaa tcattattag caacaagaat aagaacatat gacttattaa aagctgctaa
     6601 gccaacagaa aaatatcaaa aggatttatt ctcacttgaa atgtggggag gagctactta
     6661 tgatgttgca tatagatttt taaaagaatc tccttggcgt agattacaaa agctaagaga
     6721 agaaatacca agcataatgt tccaaatgtt gcttagagca tcaaatggag ttggatataa
     6781 aaactatcca gataatgtaa tagaagaatt tacaagagaa tctgctagac aaggtataga
     6841 tgtatttaga atatttgatt cattaaactg ggttgaaaat atgaaaccat ctattaatac
     6901 agctttagaa actggaaaaa ttgttgaagc aactatgtgt tatacaggtg atatactaga
     6961 taagactaaa actaaataca atctagaata ttatattaaa atggcacaag aattagaaag
     7021 cttgggagca gatataatag caataaaaga tatgtcaggt cttcttaaac catattcagc
     7081 ttacacatta gttaaagagt taaagaaaaa tgtaaaagca ccaatacatt tacacactca
     7141 tgatacaagt ggaaatggtg tagcaacttg tttaatggcg tcagaagctg gagtagacat
     7201 aattgatgct gcattagaat ctatggcagg acttacaagt cagccatcac ttaatgctat
     7261 agtagaggca cttaagaata cagagagaga tacaggaata gatttatttg gatatgatga
     7321 gttaggaaaa tattataagg atttaagaaa agtttataat aaatttgaaa gtgatttaac
     7381 taattcttgt gcagaaatat ataattttga aataccagga ggccaatata caaacttaaa
     7441 acctcaagct gatagtttag gattagttaa tagatttgat gaagttaaag aaaaatataa
     7501 agaagctaat gaagttgtag gagatataat aaaagtaact ccatcttcaa aagttgtagg
     7561 agacttagct atctttatga ctaaaaataa attagataaa gacaatataa tagaagaagg
     7621 taaaaattta tcattcccag attctgttgt ggattactgt aaaggtatga ttggtcaacc
     7681 agaaggtgga ataccaaaag atttacaaga ggtagttcta aaaggtgaag aagctataac
     7741 agtaagacca ggttcattat taccagcaga agattttgat gaaatagcta aatatctaaa
     7801 tgaaaagtat gatataaatg ctaatataag aaatgttata agttatgctt tatatccaaa
     7861 agtatatgaa gattatatca aacatctaca acattataat gatatatcta aacttgaaag
     7921 tgatgtattc ttctatggat taaataaaaa tgaagaatgt gaagtagaaa tagaagaagg
     7981 taaggtatta acaataagat tagttgaaat tggtgaagtt aaggaaaatg gatttagaac
     8041 tataggattt gagttaaatg gaatggttag agaagtagaa ataaaagata agaatttctc
     8101 tggaaaaatt aataatgttg aaaaagctga tatgaatgac cctcttcaaa ttggagcaag
     8161 cataccagga aaagttataa aaataatggt taaagaagaa gatgaagtaa aagcaaatca
     8221 accattaatc gttatagagg ctatgaagat ggaaactata atagttgcta agactgatgg
     8281 agtaattaag tcaatcaaag taaaagaaga tgatatggtt gaagataaac aattattaat
     8341 gataatgaag taatttaatt aaaataaatt aaaaagcact ctgaaatgtt taataaaaaa
     8401 tgttttagag tgtttttatt tattttaatt aaatatttgt taaaaaaaag aggacaatta
     8461 cctccagatg tagaaataaa gctagtaagg aaaatgttat ctaaaaattc taaatatagt
     8521 aaatattttc cataatattt cttaggaaat aacagcaaag ataaaataca taaatttaaa
     8581 aggtgggtgc ttatatgaag gtttatgata gtaaaatgtt gagaaatgta gcggtattag
     8641 gacatagcgg atgtggaaaa acaaatctaa tcgaaactat agcttacaca gcaaacacta
     8701 ataagatacc taaattaaca gacaaggtaa atatgactta tagcatggga ttaataccta
     8761 tagagtataa tgattataag tttaacttat tggatacccc aggatatttt gactttagcg
     8821 gagacgttgt gtcatctctt agagcaagtg atgcagcaat aatagtaata gatgcaacag
     8881 cgcctattca agttggtaca gagaaatcat tagaactaac tgagagtatt cctaagataa
     8941 tgtttataaa taaaatagat aatgaaaagg caagatacaa agatgcaata gcaatgttaa
     9001 gagaaaaata caataataag atagttccta tgataagtcc tatatacaaa gataaaaact
     9061 ttgtaaagtt acataatgtt tttgaaaaca tagatgattt agaaggtgaa tttaaggaac
     9121 aagctatgag tgttaaagaa gcattaatgg aacttatagc agaaactgat gaccaaatat
     9181 tagataaata ttttaatgga gaagaactaa ctacagaaga gatacaaaaa ggtattatta
     9241 taggtataca aagaggtgat attatacctg taatatgtgg ttcaactata aataatatag
     9301 gcacaaagga aatattagat acaatatcaa gttatttaga acctatcttt acagaggaaa
     9361 gcaaaccatt taggggatta gtatttaaaa ctatggttga cccatttgtt ggaaagatgt
     9421 catatataaa aattactgag ggtgtacttt ctaaggataa agatgttttt aatataaata
     9481 aaaatgtaaa agaaaaaata gctaatatat atacattgag aaatagtgaa ttagtagaaa
     9541 tagaaaaagc taaagctggt gatatagtag ttataactaa agttaattct ctaaaaacag
     9601 gagacactat atctgcagat aaagatgcag aagctttaga aaagatagat tttcctaaac
     9661 cacaaatata ttatgcagta actcctaaga ataaaggtga tgaagaaaaa gtagcatctg
     9721 tattaaataa actagtagaa gaggacccta cattacattg gtatagaaat acagagacaa
     9781 aacaagcact tttaggaggt caaggagaac ttcatataaa aacaattaaa aataaaatga
     9841 aagataagtt tggtgttgat gttgaattga atgaccttaa agttccttat agagaaacta
     9901 taaaaggaac agctgatgta caaggaaaac ataaaaagca atcaggtggt catggtcaat
     9961 atggagacgt aaaaataaga tttgaaagat gtgaatctga ttttgaattt acagaagaaa
    10021 tatttggtgg ttctgtacca aaacaatata taccagcagt tgaaaaaggt ttaaaagact
    10081 ctatgcaaaa aggtatatta gcaggatatc ctgttacaaa cataaaagct acgctttatg
    10141 atggttcata ccatgatgta gactcttctg aaatggcctt taaaatggca gcaagtgcag
    10201 cctttaaaaa aggtatggaa gaagcacacc caatactact agaacctata atgaaactta
    10261 agataactgt tccagaagaa tatatgggag atgtcatggg tgatataaat aagagaagag
    10321 gaaaaatatt tggaatggaa ccagatgaca aagggaaaca aataatattt gcagaagctc
    10381 cacaagcaga gacatttaaa tatgcaatag atttaagagc aatgacacaa ggtagaggat
    10441 actttgaaat ggaattagag agatatggag aggttccatc acagtttgca gaaaaaataa
    10501 ttgggttagc tactgcaaag taatgaacaa aataaataga aaaataatta atatatatta
    10561 ttaaaaatat ttttagggtt ttaactaggt ataggtttat gttaggtaaa cttataccta
    10621 gtttttatac atttttgatg aataaaaaaa tatataatac tttcttaaat attttaataa
    10681 aaagaaagta tttttttatt attattttta caaatgttta gtaataaaga tgagaaggta
    10741 tatttaaaat ataaagtatt taaagttctg aaatctaatc atgaagcaaa aaattagcat
    10801 ttggttatca ataaatataa taaataaaaa ctcataatta atgaaagaat aaattcatat
    10861 ctaaatttct ataaaaaatt taaatttata caatttaggg ctttaaaaat ttaaataata
    10921 ggtgtaaaat aatagtcaaa gaaagtcaaa aataaaaagg tgtgtggtga tattaatggc
    10981 aactatgaca gatataattg aaaaatttat taaagatttg atggaagaag ataatagcat
    11041 acaaattcaa agaaatgaac tagcaaactt gtttagttgt gcaccatcac aaataaatta
    11101 tgtcttaaca acaagattta caatagatag aggatattac attgaaagta aaaaaggtgg
    11161 cggaggatat gttcaaatag aaaaaatacg aaaaagtaaa gatgggcata ttagagaact
    11221 actaaatgaa aaaataggaa gtcagatatc ctataaaaaa gcaaaagaat tattagaagg
    11281 tttaaaagag tcggatttga ttaacgaaag agaattaaaa ttaatattat atgcaataga
    11341 tgataaatca ttgtgtatgc caatatatga attaaaagag aaggtaaggt ctaatatttt
    11401 aaagaatata ataataggat tatttagtat agaggagtga taatttatgc tatgtcaaaa
    11461 atgcaataag aataaagcat ctgtatatta taataagata gtcaatggag aaaaaacaga
    11521 gatgtattta tgtagtgagt gtgcaaaaga gaatacagaa atgaatttta acttagatat
    11581 gcctttttca atgatggata tattttctaa tttaggtttt caacctaaaa aagaacttga
    11641 ggaaaagcta gtttgtccta aatgcaatac aacatatagt gagtttaaga ataatgggag
    11701 atttggatgt agtgaatgtt ataatgcatt tagtagtcaa gtaaatccaa tgcttcaaaa
    11761 tatacatggg catatagaac atacaggaaa agcacctaaa aaatcttttt ataagataag
    11821 tgtagaaaat gagattaaag aactaaaaga agatttagat agagctataa aaaatgaaga
    11881 gtatgagtta gcagctcaat ttagagataa aattaaatat ttaaaaggtt ctattgacta
    11941 agattggagt gatattatga aagagaacat agtcatgaaa tcaagagtta ggctagctag
    12001 aaatttaaat aactatccat ttccaaataa attagataaa gaatgtgcca tggaaataat
    12061 agaaaaagta aaaaatgcgt ttattaattc taacttagaa caaaaagaag aatttgattt
    12121 ctataaaatt gaagatttag accaatctaa aaaaatgctt atggttgaag aacatataat
    12181 aagcccagat ttagcagaaa atgataaaag tgcagttata gtaaaaaaag ataaaactat
    12241 aagtataatg ataaatgaag aagaccatat aaggatacaa actatatgtg atgatttaaa
    12301 cttagaatat gcatatagtg tagcaaatga aatagatgat ttgttagaat caagtttaga
    12361 atatgccttt aacacaaagc taggttatct aacatcttgt ccaactaata caggaacagg
    12421 aatgagagct tctgtaatga tgcatttacc agcattaagt caactaggct atatggatga
    12481 actttataaa atttcatcac aaataggtat agcaattaga gggatatatg gagaacgaac
    12541 agaagcattg ggaaatatat atcaaatatc taatcaactt acacttggaa gaacagagtc
    12601 taatattata gaaaatgtaa gtggacttac aaaagatgca atttcaaaag aaataaaagc
    12661 tagagaaata ttgcaaaaaa aattaggtat aaaactagaa gataaaatat ttagatcgat
    12721 agggacactt gaaaattcaa gagtaatgag tagcgcagag gcaatgagcc atctatcaaa
    12781 tataaaaatg ggaatagaaa tgaactatat agataaacta gatttaaggg ctatagaaca
    12841 acttatgata ggtatacaac cagcgcatca gtcaataatg tataagagtg atgatgttga
    12901 gaatagagat ataaatagag caacatatat tagagaaact ttggaaaaat taagaggagg
    12961 gatgaattgt gaacttcaat agatttacac aaagagctaa aaaggcaatt gatttagcgt
    13021 ttgaatctgc taaaagtcta ggacacaata ttgtcggaag tgaacatata cttttaggtc
    13081 ttttaagaga agaagaaggt atagctgcta aagttctaag taaagttgga tttacagaag
    13141 cttatttaga aggcaaaata gttgatatgg aaggtaaggg agaagaaatc tcagaagata
    13201 tagtattaag tccaaggagt aaacaaatac ttgaattatc aggaatgttt gcaaataaat
    13261 taaaaacaaa ttatattggg actgagcata tattattagc aattattcaa gaaggtgaag
    13321 gaatagctaa taagatttta aattatgctg gagtaaatga taggacttta gcccaattaa
    13381 caattgatat gatgggtatt agtgataaaa atcaatacaa agcagaaaat agttacacgg
    13441 gcaaccagaa tcaagcagaa tctaaagtac ttgataagta tggaagaaat cttacactat
    13501 atgcaaaaca gaataaaata gaccctgtaa taggtagaga aaaagaaata caaagagtaa
    13561 tacaaatatt aagtagaaga actaaaaaca atccagtgtt gataggagac ccaggtgtag
    13621 ggaaaacagc catagctgaa ggtctggcta caaatatagc cttaggaaat gttccagaaa
    13681 cactaaaaaa caaaacttta tactctttag agatgggttc attattggct ggggctaagt
    13741 atagaggaga atttgaagaa agaattaaag aagttgtaga tgaagttgtt aaaaatggaa
    13801 acataatctt atttatagat gagatgcaca caataatagg ggctgggtct actggagaag
    13861 gctctataga tgcttcaaat atattaaagc cagccttagc aagaggagaa atacaagtta
    13921 taggtgcaac tacaattgat gaatatagaa agcatgttga aaaagattct gctctagaaa
    13981 gaagattcca accagttatg gtagatgaac caactaaaga ggactcaata aaaatattag
    14041 aaggattaag agataaatat gaagctcatc ataaagttaa aataacggat gatgctataa
    14101 aggcagctgt agaattatca actagatata tatcagacag atatctgcca gataaagcta
    14161 tagacttgat tgatgaagct gcatcaaaag taagattaaa agaaaataca cctccttcag
    14221 aaataaaaaa attagaacta gaaatagaaa atatagataa agaaaaagaa gaagcagtaa
    14281 gatgtcaaga ctttgagaaa gcagccaaaa taagagatga acaaggtcta cttaaaaaac
    14341 aattagaaga tgttagagaa agatggaaca agtcatctaa acattcagat ttagttgatg
    14401 gagaagttat tgctgaagta gtgggcttat ggacaggaat accagttaat aaaattcttg
    14461 aagaagaggc tgatagactt ttgagacttg aggaaatact gcacaataga gttataggtc
    14521 aagaacaagc agtaaaatct atttcaaagg caatcagaag gtcaagagca ggtcttaaag
    14581 accctaatag accaattggt tcatttttat ttttaggtcc aacaggagta ggtaaaacag
    14641 aattatctaa ggcattagca gaagtgcaat ttggagatga aaatcaaata attagaatag
    14701 atatgtctga atatatggaa aaacatgctg tgtcaagaat gataggttca cctccaggtt
    14761 atgtaggtca tgatgaaggt ggtcaattaa ctgaaaaagt aagaagaaat ccatattcag
    14821 ttatcttatt tgatgagata gaaaaagctc atccagatgt atttaatatt ctgttacaaa
    14881 ttctagatga tggtagacta actgattcaa aaggaagaac tgtagatttt aagaatacta
    14941 ttgtaataat gacatcaaat gttggagcat ctacaattgg tagacaaaaa actttaggat
    15001 ttagtatagc taaaggagac gaagaagaaa aatctcaata tgaaaaaatg aaagaaaata
    15061 taatgggtga attaaaacaa agattcagac cagagttttt aaacagaata gatgatataa
    15121 ttgttttcca ctcactaaat gaaaatcata tatcaaaaat agtactttta atggcagcga
    15181 aactacaaga gagattaaaa gaaatggata taaaattaga aatgagtgat gaggctgtta
    15241 aattaatttc taaatctgga tttgacttag aatatggagc aagacctctt aaaagagctt
    15301 tacaaaaaga gttggaagat gagctatctg aagcaatctt aaaaggtaat gtgaaaaagg
    15361 gaagtaatgt agtagcaaaa gtcaaagatg aaaaaatagt ttttgaaact aaataattta
    15421 atagagttgc attttaaaat attgcaaata taagttttat aatctaaaca aaattttaag
    15481 ggcaatgaat ctataataat tcattgtcct taattatttt atttaatctt tacatattgt
    15541 ctttcagata tgctaaaatt atagtataga ataaaaaatt aaggatgata aaagtatggc
    15601 aaaaataaaa acaaaatatg tatgtcaatc ttgcggatat gaaacagcta aatggcttgg
    15661 taaatgtcca gaatgtacaa aatggaatac atttgtagaa gaaatagacc aaaaaagcac
    15721 caaaaaagag gtctttataa tagataaatc ttcatctaaa ccagtcagta taaactcaat
    15781 agagagtaaa gaagaagagc ggtttacaac agatataaat gaattagata gagtgcttgg
    15841 aggaggtata gttaaaggct ctttagtgct tgttggtggt gaccctggta taggtaaatc
    15901 aacactttta atccaagtat ctagtaatgt tgccaattta ggaaaaactg ttttatatat
    15961 aactggagaa gaatcagaat ctcaaataaa aatgagagct aaaagattag gtattaactc
    16021 agaaaattta tatatatttg ctgaaaataa tttaagtata attgaatctt atttagaaag
    16081 tgtaaatcca gaattaataa ttctagactc aattcaaact gtcttcagtc cagaaatatc
    16141 ctcggcacca ggaactgtca gccaaattaa agaaggtact tcaaaattca tgaaaatttc
    16201 taaaaaaatg ggaatttcaa cgttcatagt tgggcatgtt acaaaagaag gttcactagc
    16261 aggaccaaaa ttattagagc atatggttga tactgttcta tattttgaag gagaaagata
    16321 taatacttat agattagtaa gagctgttaa aaatcgattt ggttcaacta atgaacttgg
    16381 agtatttgaa atgagggatt taggattagt agaacttgat aatccatcta aaattttaat
    16441 ctctgaaaaa ccaaaagatg tagcaggttc agtaataata tctactgtag aaggtacgag
    16501 accaatgtta cttgaattgc aagcattagt ttctcctaca agttttggta taccaaaaag
    16561 aacatctaca ggagtcgatt acaatagagt aggtatgtta ttagcagtat tagaaaaacg
    16621 tgttggtttg caaatacaaa atcaagatgt ctatataaac atagttggag gaataaagat
    16681 aaatgaaccc tcaattgatt tagggatagc catttctgta gcatctagtt ttagaaatat
    16741 accgatagat gaagatatag ctgttacagg agaagtggga cttacaggag aagttagagc
    16801 agtaagcttt atagaaaaga gaatagctga gtgtaaaaaa cttggattta aaaagatagt
    16861 agttcctaga agtaattatg atgttgtaaa agagaccaaa ggcatagaaa tatggccagt
    16921 agataactta agacaagcta taaatatagt acttgggagg aatcaataaa tatggagaat
    16981 tttctagata ataaaaatat gctatatgca ttaaaaatga tatctcctgg aactccactt
    17041 agattaggtc taaacaatgt actaagagct aagactggtg gattaattgt aattgcaaca
    17101 aacgaagatg taatgaaaat agtagatgga ggatttgcta taaatgcaga atattcacca
    17161 tcatatctat atgaattagc taaaatggat ggagctatag ttttaagtgg tgatgtaaag
    17221 aaaatattat ttgctaatgc acaacttata cctgactatt ttatagaaac atcagagaca
    17281 ggaacaagac atagaacagc agaaagagta gcaaaacaaa ctggtgctat agtcatagga
    17341 atttcacaaa gaagaaatgt tataacagtt tatagaggaa atgagaagta tgtagtcgaa
    17401 gatatatcta agatatttac taaggcaaat caggctatac aaactctgga aaaatataag
    17461 acagtattgg accaagctgt aacaaattta aatgccttag agtttaatga tttggtaact
    17521 atttatgatg ttgcattagt catgcaaaag atggaaatgg taatgagagt tacaagtata
    17581 attgaaaaat atgtgataga attgggtgat gaaggaactt tagtaagtat gcaattagaa
    17641 gaattaatgg gtacaaccag aatagaccag aaattaatat tcaaagatta taataaagaa
    17701 aacacagaaa taaaagaact tatgaaaaag gtcaaaaatt taaattcaga agaactaata
    17761 gaattggtca atatggcaaa actattaggg tatagtggtt tttcagaaag tatggatatg
    17821 cctataaaaa caagaggtta taggattctt agcaaaatac atagactacc aacagcaata
    17881 atagaaaact tagtaaatta ttttgaaaac tttcaacaaa ttttagatgc atctattgaa
    17941 gaattagatg aggttgaagg aataggtgaa ataagagcaa catatataaa aaatggactc
    18001 ataaaaatga aacaattagt cttattagat agacacatat gatgaatttt aaatgaattt
    18061 tactactttt ttcttgaata taatatacac tttggtctat aatagataag tataactagg
    18121 gggtgaagca ttgataagga aagtaacaag aatattgttt attttacttg gatttacaat
    18181 aggtataaca acctatttaa cattaatgaa agattttgaa atattaacat ttggaaaaga
    18241 aacatatggt tatatcgcag ctgtagtagc aggtataata atagccattt taggatatct
    18301 tatagaacca tgggttgtaa ataaagtaaa agaaattgct aaaatagtgg ataaagaatt
    18361 gtcaaaatat cctcagactg atatattgtt agggtctatg ggtcttatag taggatttgt
    18421 tattgcgtat ttattaagtg gattagtaaa tagaatacca atagtaggtg gaatactatc
    18481 cttactatta tatttattcc taggatatct tggaatgaaa gttgcgctta aaagtaaaaa
    18541 tgatttattt aatattggaa aactaggaag attagcaaat cctattaagg ataaagataa
    18601 ggataaagaa aataaaaaag aagtaaaagc cattcctcca aaagtattag atacaagtgt
    18661 aataattgat ggaagaatag ctgatatttg taagactggg tttatagaag gaaaacttat
    18721 tattcctgct tttgttttgg aggaactaag acatattgca gattcttcag atgatttaaa
    18781 aagagtaaga ggtagaagag gtctggatat attaaatata attcagaaag aattaaatat
    18841 agaagttgaa ataagtgaga gagactttga tgatatagca gaagttgata gtaaattatt
    18901 aaagcttgct caggttttaa atggaaaagt tgttacaaat gattataact taaataaagt
    18961 tgctcaattc caaggtgttg aagtattaaa tataaatgag ttagctaatg ctataaaacc
    19021 agttgctata ccaggtgaag atatggttgt tcaagtggtt aaagaaggaa aagaggctca
    19081 acaaggtgtt gcatatctag atgatggaac tatgatagtt gttgatggag gaagaaaata
    19141 tatgaatgag actataaaag tactcgttac ttctgttctt caaactcctg caggtagaat
    19201 gatatttgca aaacctaaaa attaatatgt tcaaagaaac ctataatttg ggctgtttat
    19261 tttaaataga tggtccaaat ttatttattt ataacgataa tgtttaatgt attcttaaag
    19321 atggggattt aaatcagaaa gcagggagaa tttatgagaa aaaagattat atctttctta
    19381 gcaacattta tcataatatt aacatcagca agtcagtata gttttgcaga tgatatatca
    19441 acacgtggaa aagtaatatt tatagatatg aatagaacta gtatgagtaa tatgttaaga
    19501 ataaaatcat taagagaaga actagacaat agagggtata ttggcttgat gaatataaga
    19561 ggcgataaag gaagtgatga tagaaggtca tatgctagta tgggtgctgg tggtagagct
    19621 aatgtggcta atgaagaaga tattaatttt gaaagctcga gtaaggatag aaatatagtt
    19681 tttgaatcag ctacaggaaa aagtgcaaaa ggtataaata atttaactat aaataagtct
    19741 ataaatgaaa atttaaactt tggagagtat ggttctgtat tagggtcatt aggacaaagt
    19801 ttatctgata atggattaaa agcatctgta ttgggtaatt cagatataat tgaaaatggt
    19861 caacttataa aaaatagaaa cttatgtcta actgcaatgg atgaatatgg aagaatacct
    19921 aatggaaatg tagatactat aaataaaaaa gacttatcta tgccttatgg aatatcaact
    19981 gattatgata aattgattgt agagactaaa gaagcttaca aaaataatga tgtgattttt
    20041 gtggagcttg gagacacata tagattagat ttatataaac caaatttaaa tgaaaaaaca
    20101 tatgagagta tgaaagataa catagaaaaa aatatagata tatatttaaa aaatatattt
    20161 tctatggttg aagaaaatga cactgtctac atagcaagtg catttccaag tgatttagat
    20221 tataaaaata aaagaagact ttctccaata ataaaattaa atggagaagg gaaaggtata
    20281 ttatcatctt caactactag aagagaaggt attgtaacaa atttagatgt tggggttgaa
    20341 atattagaca actttaatat aaaaaatcaa aatatggttg gaagaaaata tgaacttatt
    20401 aacagagatg ataataaaga atttttgatg gatgaatatc aaaagatagt atctatttca
    20461 agcataaggt ctacaatact gaatggtttt gtaagtatag ttttcttgtc atggatagtt
    20521 gctatgattg caattttatt tagaaagcat atatcaaaaa attataaaga aacaacattt
    20581 tttatattaa aggaactttt aaaaatagga atagtaatgc cactgtcatt tatgataaca
    20641 ccaataatga atttcaaaac accattagca atatctttag gaataattat aataacactt
    20701 actatatatc ttataagtaa agttttaata aagaacgatt taaaaaacat gctgtttttt
    20761 actggattaa ccatagtaat tatggttatt gatgctgggt ttggctcata tttaatgaaa
    20821 agcaatgtta tgagttatga ttgtataatt ggagctagat attatggagt aggaaatgag
    20881 tatcaaggtg tggcaatcgg tagtgcaata tttacatttg caattttact tacatataaa
    20941 aatataccaa agtggtctat aatagttttt tctttggtta tcttaattac aagtgcatct
    21001 ccaattatgg gagcaaatgt tggaagtgct atttctgaat gtgttgcatt tttactattt
    21061 atactattga tatataatgt aaagatagat ttcaaaaaaa tcgtattatt aggtatagca
    21121 gtagtatttg tattaggagt ttttgtagca atagatatga ttttgggaag taattcacat
    21181 ttaggtatgt ttgtaaaaga aatttacttt aatggaccag gagaaataat acaaacattt
    21241 agtagaaaga tagagatgaa tttaaagcta gctcaaacca gtgcatgggt aaatatactg
    21301 ttaactggaa taggtgtaat tttagttctt atgattaatc agattagata ctttaagcaa
    21361 ctgatggatg agtatccaat tgtatttaaa ggatttatag catcaatagc aggatgttta
    21421 gtaactcttt tagtgaatga ctctgggatt gtgtcatcag ctacagcctc tatttatgta
    21481 attgtaccta tgataacatt aagtataaat ctcacagcat taaaagaata agtactgtat
    21541 gaataatatg taaatatgaa cacattaatt taacttcttt atttacaaaa taaagaagaa
    21601 agtttataaa aaataaatta taataaaaaa gagtttatct caaaattgat tattctaatc
    21661 aaattttaag gtaaactttt tttattataa agataaataa catataacct catattttag
    21721 gtagattttt ttcttatatg tttaggagtg catttaaatt tttccttaaa aattctgtaa
    21781 aataattttg tattagtaaa tccatgagct tctaatatac tgcataaggt atcatttgta
    21841 ttaattaagt ctttataaat atgagaaata cgcacatcat ttaaatactc taagtaagtt
    21901 tgtcccatat actgtttaaa ttttctacaa aaatattctg gttgtaagtg tgctacctta
    21961 gcgattttgt taatagaaat aggcattttg taattaatct ttgtatattc taaaactggt
    22021 tcaaattttg acaaatcaag tactgttttt ttcatatttt tattacctac agatatttta
    22081 aagtttgtat ataattcaaa taaaagttca aataataagc ttgtaaagag cagattagct
    22141 gcaggaggag aactaagttt aatttctctc atgttttcta atataagttt aactttactt
    22201 gaattttttt tatagttatt gtcattggca ttaaccttaa agtcaaaata gcaattttta
    22261 aaatcaggga tatatttgtt tagaaaagac aatggtactt gcaataatat agaggtattt
    22321 tgataaatac atctagttga gtgaataatt ccagaattaa tgaacataca ctcattagct
    22381 tttaaattat aagtgtaacc ctccatgtta atttgtaaat caccagaagt tatataaatt
    22441 atttctaatg agttatgcca atgatttgct acataactac cactatcatc aaatagtttg
    22501 aaattaacat ctaaattatc aataggttca attatttcat gtgaaaaatt atttctgttc
    22561 ataataatat cctccttata attttaaatt ataaatagta aatttataat gttcaatatt
    22621 aaaaagataa tatagactaa taaattagat aataaacata cttaagaaaa cggataaaat
    22681 caaatataat aactgtatat taaaataact taaaaaaatt attttattat aagtgcaaaa
    22741 tatacctatt taaataagat aagaggggag gattttatgt gtatataaaa ggtgtaaatt
    22801 taggaggttg gttagtttta gaaaaatgga tgacctcaag tttatttgaa ggcacagaag
    22861 ctgaagatga atactattta ccaagacagc tttcaagaga ggcatatgaa tctagaatta
    22921 agactcatcg tagtgaatac atcacagaga gagattttgc aacgattaaa tctatgggat
    22981 ttaattcagt tcgtatacca gtaccatact ttatttttgg agattgtgaa ccattcatag
    23041 gatgtgttaa agaacttgat aaagcatttg cttgggcaga taagtatggg cttagtatat
    23101 tgatagactt acatacagtt ccaggaagcc aaaatggatt tgataatgga ggaatatcag
    23161 gtatatgtag ctggtcacaa aatccagaat atgtagcgtt tacattaaat gtattagaaa
    23221 aacttgcaaa acgttatggg atgaggcatg aattgtatgg aatccaaatt ttaaatgagc
    23281 ctataacaga aagaatgtgg aacataatga atgttccaaa tcggtttaag gctgtagata
    23341 aggagatggc aagggggagt aaacctaatt cattagagtt tttacgtgat ttttatataa
    23401 aagcatatag ggtaatgcgt ccttatatga gagaggaaaa cgttatagtg tttcatgatg
    23461 catttgaatt aaaagcatgg aaagacttta tgagagagga agaatttaaa aatgttgttc
    23521 tagatacaca ccaatatttg atgttagctg aagcagatgg atgtgaacaa agtattgata
    23581 gctatctcaa atatattaga gaaaattatg ctaaagatat tttacaaatg caaaaatatt
    23641 ttcctgttat atgtggggag tggagcttat ttaattcata tgcttgtggt atagatacaa
    23701 atggaggtca atcacctcta aatgggattg aatcaaatat agataaatta tcaaaagacg
    23761 ataaaagaga attatatagg aagatagcaa aagcacagct tgatgcatgg agaaatggaa
    23821 gtggtcacta ctattggaat tataagttat tactagatac agttaatgaa gaaggttgga
    23881 taggatggga tagttgggac ctaggcaaat gtgtagctca agaatggtat cctatagaat
    23941 attaaactaa aattggagga aaagatatga ttaaaaaccc tatacttcca ggttttaatc
    24001 cagatccttg tatttgtaga aagggagatg attattatct tgtagtttca tcttttgaat
    24061 ggtttccagg tatacctgtt tatcactcta aagacttaaa aaattgggag ttatatactc
    24121 atattttgac agatgaaaca aaaatagatt tgaagaagct tccttcatct aaaggaattt
    24181 gggcaccttg tttgacttat tgtgaagaag aagatttatt ttacatagtt tatggaatta
    24241 tgaactctat gaatgcaaga tattttgatg ttgataatta tttaattaca tcaaaagata
    24301 taaagggaga gtggagtgaa ccagtatatc tacactcatc aggttttgat gcgtctattt
    24361 ttcatgatga tgatggtaaa aagtggattg catcattaga ctgggagact agagaaggat
    24421 atgaaaaacc tggtgttata tgtttagtgg aatactgtac aaaaaagaaa gaaatagtag
    24481 gatatccaaa gagaatatgg tcagggggaa ctgatagagg ttgtattgaa gcaccacata
    24541 taacaaagcg tggagattac tattatataa tgtgtgcaga aggtggaact ggatatggtc
    24601 atagtgtaac tatgggaagg gctaaaaata tatggggacc ttatgaaaaa gattcaatga
    24661 atccaattgt aacttcaatt ccaggcgatt tttatgaaag acatgaccct gaccatctaa
    24721 agcctaaata ctataatcca gaatcaaaac ttcaaaaatc tggtcatgga agctatatag
    24781 aaacaacatc aggtgaagta tatttagttc atctaacatc tagaccattt gtaccagagt
    24841 taaggtgtac tttaggtaga gaaacagcta ttcaaaaaat gaaatggaca aaggataatt
    24901 ggcttcgaat ggaagatgaa agtaatttgg caaaagaata cgtatcagaa agtaaactag
    24961 aagaacattt agtttcatct ataccaagtt ttgatgactt tgattcaaat gaattaggtt
    25021 tacagtacta tgctcctagg atttcaccat tatcatttgc agatgttaag tctagaccag
    25081 gatatgtaag gattagagga caagaatcaa gaacatcatt aaataaagta agcatattgg
    25141 caagaaaatt aacttcagta tatgcaagaa taaccactaa gatggagttt tacccagagg
    25201 tacatcagca tagtgcgggt ttgataatgt attatgataa tatgaattat ataaatttaa
    25261 gaaagtatta tagtgaaaca ttgggtcaaa gtgcactatc aataatacat cttgaaaatg
    25321 gggagaaaac ggaattttta aatactagga taccaataaa agatatacca atatatcttc
    25381 gtctgtatat acaaggtcgt aagtcatatt ttgaatggag ttatgatgaa aaaaactacc
    25441 agagaatagg aaaggtattt gatacaacta agttttcaga tgagtactgt aaatatggag
    25501 aatttacagg tacgtttata ggattaactt gtgcagatcg tgtaaagcat aaacactatg
    25561 ctgattttga tttttttgaa tatatagtag atgaatcaaa agatgtcgat taataaaatc
    25621 attactaaaa ttttcagagc aaaggtaaat cttatgctct gaaataagaa acattagaag
    25681 gcaaatctta caaaaaggag tatggtatta tgaatacaga agttaatgtt aaagcaacta
    25741 cgattagata tgaaaagatt tcattttttg aaagaatttc ttatggttgt ggggatttgg
    25801 ggtgcaatat catatactca gctatgtcag catttttgct gttttattat actaattatg
    25861 cagatgttag tgcagcagct gtaggtagta ttatgcttgt atctaggata ctggatgggt
    25921 ttagtgactt aacaatggga attatagtgg atagaactaa atccaaatac ggtaaggcta
    25981 ggccttggat tttgagaatg gcgattcctt ttgctatagc agcagtatta ttattttctg
    26041 ttccatcaaa tcttggtatc acttctaaat taatttatat ttttattaca tataacttag
    26101 tttctactgt tatttataca gcaatcaatg taccctatgc aactctaaat tcacttataa
    26161 ctcaaaacca gtatgaaaga ggagtattaa gtatatttag aatgatatta gccacttgtg
    26221 gtaccttact aataaatgga cttacactac ctctagttga atactttgga aataatttat
    26281 cagcatggac taaaacattc tttgtatttg gaatagcatc tataatggta ttttttataa
    26341 catttactgg aaccaaggaa agagtaaaag cagttaagca agataggaat gaagtaatac
    26401 catttaaaat aggtattaaa tctttattta gaaataagta ctggatacaa ataacattat
    26461 gtttagtttg catatttatt gtatttgcga taaatggtgg ttcatccgta tattatgcaa
    26521 agtttatttt gggagatgaa aaattatttg gtcctataaa tatggtttca aatatatccc
    26581 aaattatagc tatgtttatg gttgctcctt ttataaaaaa atttggaaaa agaaatgtat
    26641 taatagtagg ttcaataata ttaatatcgt caaatgttat gtttataata gttggacaaa
    26701 atcatatagg agtaatattt gcaagtgcta taaagggaat aggaagtgct ggaatagcat
    26761 caacaatgtt tgctattgtt tcagatacta ttgaatatgg agaatggaag actggatata
    26821 gaactgaggg gcttataaat agtgcatcta gttttgggtt taaagtggga aatggattag
    26881 gttcagcaat attaggtgca gtattatcaa ttggtggtta tgtaggaaca tcagctactc
    26941 aaagtgattt agctatagta tcaataaaag tatgttttat atacctacct atatttataa
    27001 caatattaca aattatcata atgtcttttt ataagcttga taaagaatat agtactatac
    27061 ttaatgaatt aaatactaaa taatgaaaaa gtttcttata aagtcttaag tttaattaag
    27121 gtctattgta aatgttccct ataaaaacta ttatatattt atgattattg aagaattttt
    27181 agagaagcga ataataaaaa tataagaaga ctttaaatga tgaaatggtt ggttttttag
    27241 aaatgattta ataagttatt gttatttaaa gcattataag tggacaatta atttttaatt
    27301 tatggagatg atatttaaca attagaagta atatataata tgcactaaga agcaattttt
    27361 caactattta tgaaggtggt ggtacaagaa aagtatcacc ttttttttgt tatacaaaaa
    27421 aacagttgac tttttatatg aaccccttct ataatgttac tgaaattaag taaaatgatt
    27481 gaacaaatgt aaagataatt gaaaagcttg attttggagg aaagcaaatg aaaaaaattg
    27541 gagacttaag attaatgatg aagtgttgtt cactttatta cgaagataat ttgaatcaac
    27601 aggaaatagc aaatcaattg ggaatatcaa gaccaacaat atcaagaata ttaaaagaag
    27661 cttttgaaca aggaatagta aaaatacaga tagttgatgt tttgaaaaat gattaccaaa
    27721 aaatagagag aagtttggag agaaaatata aattaaaaga agtaatagta gtagatgata
    27781 aacaggatgc actaacacaa aaacaagaac tagcaagagc tgtatctgaa tatttaacta
    27841 gagtagtaaa ggaaaatgat ataataggtg tttcaatagg aacaacacta aaagaaatac
    27901 caagatatgt ggaaaaaagt aactgtaaaa atgttacatt tatacctcta ttaggaggga
    27961 ttggagacaa tgaaatagat attcatgcaa atcaaatagc cgtaagttta gcgagggctt
    28021 ttggaggtga ttttaaatta ttacatgctc cagctgttat gtctgacttg tctacaaaag
    28081 agaaactttg caaagacgaa aaaattaaag aagtactaga tttaatagat aaaacaacta
    28141 tagcaattgt gggtatagga aatcctatga gtttaaactc aacaataatg gcttctggat
    28201 acatgaatga agcagatata gaagatttaa aaaaatacaa ctcaataggt gcaatttgtt
    28261 tacaagcctt tgataaagaa ggaaaaactt ctatacttga atttaatcaa agagtattag
    28321 gagtgaaact ggaagattta aaaaagataa aaagaacaat aggtgtcgct tcaggagatg
    28381 aaaaaataga ggcaataaag gcatcattaa aagcgaaatt tataaatagt ttagcaatta
    28441 atcacagttt agcacttaaa ttattagaag attgtgacta atagaaagat agataaaagt
    28501 taacaggagg gaatgtatat gtcaatatca aaagaaacat tgcttgaaat gtataagaga
    28561 atgaatcagg ctagaaaatt tgaagagaaa gttagttggt tttttgcaag aggtatggtt
    28621 catggaacaa ctcacttatc tgtaggtcaa gaagcgtcaa gtgtggcggc tgtaatggct
    28681 ttagaaaaag gagatttagt atcattaact catagaggac atagtcaatt tataggaatg
    28741 ggaatagacc taaataaaat gatggctgaa cttatgggta aagaaactgg attttgtaag
    28801 ggaaaaggag gctcaatgca tatagcagat attgagtcag gtaacttagg agcaaatgga
    28861 gttgtaggtg gaggtcttac aatagctcca ggagctgcac ttacacaaca gtataaaaaa
    28921 acaggcaaaa tagttttatg tagttttggt gatggagcat caaatgaagg tacttttcat
    28981 gaaggaataa atttatcttc aatatggaaa ttgccaataa tattttactg tgagaataat
    29041 ttatacggta tgtcaacaag cataaaacgt catatgaata tagaaagtat tgccactaga
    29101 gcagcttctt atggaataga aggaatatca atagatggat ataacccaat agaagtatac
    29161 gaaacagtac aaaaagctgc tgagaagtgt agaagaggag aaggacctgt attaattgaa
    29221 agtagaactt atagatggtt aggtcattca aaatcagatg caaatgtata tagaacaaaa
    29281 gaagaaatag aatcatggaa agcaaaagac cctattgaat ttttaaaaaa ttatcttata
    29341 gaaaataatc tatctaatga agatgaatta gataagattc aagaatttgc aaagcaatca
    29401 atagaagatg cagtagagtt tgctcaaaat agcccaaatc caaaaataga gtctttatta
    29461 gaggatgtat atgcagacta agaatggtaa aggaggaaaa taaaaatgag tacaagggaa
    29521 ttaacatatg cacaagcaat aaaggaagca atgtcagaag aaatgagaag agatgaaaat
    29581 gtaatattta tgggagaaga tataggaata tatggaggag catttggtgt atcagttggt
    29641 atgatagatg aatttggacc agaaagagta agggatactc ctatatcaga agcagctata
    29701 gcaggagcgg cagcaggtgc agcagcaact ggcttaagac ctattatgga agtaatgttt
    29761 atggactttg taacaatttc tatggatgca attgtaaatc aagcggcaaa aatgagatat
    29821 atgtttggag gaaaagcaca agttccaatg gttgttagat gcccaggagg ttctggaact
    29881 ggttcagcag agcaacattc acaaagttta gaagcttggt tttgtcatgt accaggagta
    29941 aaagtagttg caccttctac accagcagat gctaagggat tgttgaaagc agctataaga
    30001 gataataatc cagtcatatt tgttgagaat aaattgttat acagaaaaaa aggttttgtg
    30061 ccagaagatg attatgtaat tgaaattgga aaggcagata ttaaaagaga gggtactgat
    30121 gttacagtaa ttacttatgg aagaatgtta caaagtgttg aagaagtagc agagactcta
    30181 tcaaaagaaa atataaatgt tgaaattata gatttaagaa ctttatatcc attagataaa
    30241 gagactatag taaagagtgt atgtaaaact gggagagttt taatttgcca tgaagcagct
    30301 aagactggtg ggctaggtgg agaaatatca gccctgataa cagaaagtga atcatttgat
    30361 tatttagatg ctccagtaaa aagaatttgt ggaaaagatg tgccaatacc atataatcca
    30421 gagttagaaa aagcagttgt cccaagagtt gatgaaatag aagaagcaat aaaatcttta
    30481 attgttagat aaggagggat agatatggtg gcaaataaaa taaaagccac accagcagct
    30541 agaagtcagg caagaaaaga caatataaaa ttagataggc tgattggaag tggagaaaat
    30601 ggaaggattc atttagtaga tgtgttaaat tatttaaaag ataataaagc aaatactact
    30661 cctttagcta gaagaatagc agaagattta aatatagatt tagaaactat tttaggtact
    30721 ggatataatg gaaagataag aaagtgtgat gtggaaaaat taactgcaaa agaaactatt
    30781 gtatcaacta atacatctaa atctagtgag aaaaaagagc tgaaaattga aaatgaaaat
    30841 tcaagcatgt ttaacacagt agaaggaata tttgaaaagc caaatccaat gagagctact
    30901 gtagcaaaac gaatgtcaga aagttatttt agtgctcctg tatttacatt caatatagaa
    30961 gtagatgcta cagaattgaa ggtacttaga gcaaagttaa tcgacacagt aaaagaatca
    31021 actggagtaa aattaactat gacagattta atagtaatgg cagtatctaa aatacttcca
    31081 aatcatcaag ccttaaattc agcatggaca gatgagggta tatttagata taaagatgta
    31141 aatatagcta ttgcagttgg tttagatgaa ggcttatatg ttccggtagt aaaaaatgca
    31201 aacaaaaagt ctttaaaaga aatagcaaaa gaaagcaaag aacttgcgga aaaagtaaag
    31261 acagggaaac ttatgccagc agaccaagaa ggtaatacat ttacaataag taatgttgga
    31321 atgtatggta taactacatt tacacctata ataaatatgc cttcaagtgc aatcttaggg
    31381 gtaggtgcta cacaagataa gtttgttcca gtaaatggag aggctaaaat aaaacctata
    31441 atgaacttat cattaacatc agaccataga gtaatagatg gaacagtagc tgcaaaattc
    31501 ttaaaagatt taaaagaatt gttagaaaat cctttatcaa tgcttgtata ggttttgata
    31561 ttttgttact gatataaata gtttgaatag attatattat tataattgtt tcagtaaatt
    31621 taaagctttt atatattaag aattattttt aacagtaact atatgtgaat atgatttgct
    31681 taattaaaat ataagaaaat aaataggagg agagcctatg tcagtagaag taataatgcc
    31741 aaaagcagga gtagccatgg aagaaggtac tatagtatct tggctaaagc aagaaggcga
    31801 agaagtaaag attggagaac ctatactaga aataacaaca gataaagtta atatggagat
    31861 agagtcagaa ggagaaggta cacttgctgt aatcatacat aaagaggaag gtgaagtatt
    31921 accagtattt acagtaatag gtgtaatagc agaaaaaggg gaaaatcaag aggaagtaaa
    31981 agctaaatat ttatctggaa atgtttcaaa agaagataca gttaaagaga atcaaaatat
    32041 agaagttaaa gaagaaaaaa taaacaaaaa agagtgtaat catgattatg atgtagtggt
    32101 aataggagga ggacctgggg gatatttatc agcattaaaa gcagctcttt taggtggaag
    32161 agttgcttta gttgaagaaa atatactagg aggtacttgc ttaaatagag gttgtatccc
    32221 tacaaagact tatataaaaa cagcagaaat attagaagaa attgaccaat tatcaaagag
    32281 aggtgtaaaa gtaactgtag ataaagaaca ggacattaag aaggccataa aatataaaaa
    32341 tagagtagtt aaaaaactta cagctggtgt tggcggatta ttaaaaagta gagatgtaga
    32401 tgtgtttaat cttaaagcta gtgttaaaga agagcataaa gtaattttat cagatggaaa
    32461 agttttagat acagaaaata taataattgc tacaggttcc aaagtaagga tattacctat
    32521 aaaaggaata gaatcaaatt taataataac tagtacagaa gcattagatt tggaaacagt
    32581 gccagaagaa ttagtaataa ttggtggtgg agtgattggt tgtgaatttg cagagatatt
    32641 caattcaaga ggttctaaag taactatagt agaaatggaa gatagagtaa tcccaagaat
    32701 ggataaagag ttaagcgaat ctttaaaata ttctcttagt aaaaaaggta taaatgtatt
    32761 aactaaaaag aaagtttctg aatttaaaga agaaggaaat aacatattag tgtgcattga
    32821 aggtgaagag ccaataaaag cagatttatg tctatatgct ataggaagag aagctaatct
    32881 ttctggcata gaggacttag atattaaaat agataaaggt tcaatagttg taaatagtaa
    32941 aatggaaaca agtataccaa gcatatatgc agtaggtgat gttactggag gtgtaatgtt
    33001 agctcatgct gcttttaaaa tgggagaggt agcagcttca aatgctctag gtgtgaataa
    33061 agaagttgac ttaggtgcat tacctagttg tgtttataca attccagaag tagcatcaat
    33121 tggtattaca gaagaggatg ctagaaaaaa atataatgta aaagttggta agtttaattt
    33181 tgctggaaat ggaagagctt tagcttctgg tcaagaacaa ggatatgtga aggtagtagc
    33241 agatgctaaa tatggagaaa ttttaggtat acatatgttt ggatgtggtg tagcagaact
    33301 tataaatcat gcagcatcat ttaaagcgtt agagatacct actgatgaag caagtgagtt
    33361 gatatttggt catccatgta cttcagaagc tttaatggaa gctttggcag atgtaaatgg
    33421 agaatgttta cacttaccta aaaaataaat ctaaacataa attattaatt taagtctatt
    33481 attaatatta atttatatca taaaatttta aaacacttac ttagccatac cccttattat
    33541 gcaaataatt tatttgcata ataaggggta ttttttatgg aaaaatatag aatatagaat
    33601 ccttgtagtt tttgttgtac aaaaaagata gtaatataaa ataacattat cattttagaa
    33661 tgatttcaat tgtaattagg aaaagtgatt tttttgtttt taaaaaaagt attatttgta
    33721 aaatataaaa aaaaatttct taatatttga ataaaagaat agcttaattt aaatgttatt
    33781 actttataaa aaaataataa gtacaaaaaa aacaatatat ggcatataag ttgctatata
    33841 aataataagg agggaacaat tataaatgag gcaaagaata aaaaacttaa ttgaaaatga
    33901 agataagaaa aatcctttaa cagatgaagc gatttcaagt aggttaaata ttcgaagaga
    33961 agatgttacc tttttgagaa atgagttaaa aatagaagat tcaagacaaa gaagaaaagt
    34021 tgttttaatt aaagctataa aagatatttt aaaagaagaa aaaactataa ataaaaatga
    34081 tataacaaga agattaaaca gcattgggtt tacagtatca agatttacag tcatacaata
    34141 cttaaaagag attcaagatt ctggtgacct aaaacttgat aaagagagtg atgataaaaa
    34201 agaaaaaata atcaataata gagaaaataa agatatagca tttgataaac ttataggtag
    34261 tagtggtagc ttaagtacaa ttataaaact agccaaagca gcaattcttt atccacctca
    34321 tggtcttcat actattatta tggggccaac tggtgttgga aagagtgagt tagcagagtg
    34381 tatgtataaa tttgcagtag aatcatccag attccctaag aatgcaccat ttatagtttt
    34441 taatgcagca gattattcag agaatccaca gcttttgcta tcacagttat ttggacacgt
    34501 taaaggtgct tttacaggag ctgatgagaa caaagaagga ttagtatcaa aggcagatgg
    34561 aggaatattg tttatagatg aaatacatag attacctcat gaaggtcaag aaatactatt
    34621 tcaactgatt gataaaggta tggtcagaaa gttaggagaa acaagactaa cacataaaat
    34681 agatgtaatg attatttcag caacttctga gccaatagat tctcatttac ttaatacatt
    34741 taaaagacgt atacctgtaa caattgaaat accagaatta gttgcaagac cattaaatga
    34801 gaggtttgac attataaata atttcttctt aatagaagct caaagaatgg gagcaaacat
    34861 acatataaag tcagatgtat taaaagctct tatgctttac gattgtatag gtaatgtagg
    34921 tcaacttaga agtgatattc aagtagcttg tgctagggga ctattaaacc aacttacaaa
    34981 caaactaaaa gaagtaaata taagcatatc agatgtacct ggatatgtaa aacaaggtct
    35041 tataaaaatc agaaattgta gaggaaaaat agaaaattat gtagatgggg atttaattat
    35101 agactcaaat cttcaaggtg agatagaaaa gaaaaatgaa gatatatata cttttccaga
    35161 tgaaatatac aaacttactg aaagaagaca tattgattta ttggaacaag gtttagattt
    35221 tgatgttata aatagaatca ttggaggaga attagaaagt tgcattcaaa aatacataaa
    35281 acaagttaaa aaagtttcta ttagtaacaa tcttccagat ataagtccaa ttgttggaaa
    35341 atatatcgta gagctaactt atgaagtaat caaaatagca tcacaatacc taagttcact
    35401 agaaccgaat ctacatttat gtttagcagt tcacctaaaa gcagcttttg agagattaaa
    35461 agaaggaaaa gtaatatcaa atgctcattt gaaagaaata ataggaaatt atccagtaga
    35521 atattctgta gcagaaaagg tagctcaata tttaaaggaa agatataaca taaacttacc
    35581 aaaagaagaa ataggtataa tagcaatata ttttaaaatg agtagtagaa gagatatgtc
    35641 tgaacaaaaa aagataggcg tacttcttat ggctcacgga agagtagcat cttctgtatg
    35701 tgaggttaca aataaactat taggaattaa tcacgcaagg tatttagata tgccatttga
    35761 gaagaagccg gaagaaatga ttgaagaagc tactacaata ataaaagata ttgatgaagg
    35821 tagaggtgtg attatattag tagatatggg ttcactttca tattttggag atattatttc
    35881 tggaaagact ggaattaata taaggacaat atctagattt gatacattgc tttctataga
    35941 ggttatacga agagcagttt taccagattt aacaatagac gagatagttg atgaagttaa
    36001 aggtatagag ccacaagtaa aagaaaaggt tagtaaatgt aacaatgcaa aagcagactt
    36061 tagaaaacct ataattttaa cattatgtat tactggatta gggggagctt gtaagataaa
    36121 aaaaatctta gaggaaaata ttaaaagtat aactgaaaag gttgatatag aacctatagg
    36181 tatgattgaa tatccaagtc ttgatttaga gataaaacaa ataagcaatg ataaaaatgt
    36241 aatcgccata gttggaacaa ttgaccctca gatagcaaat atcccttttt ttagtctagt
    36301 agacataaat gataaagcaa agttaaatca aataaataac ctaattgata caaatcaaat
    36361 tggtgaaaca aataaaaaat atgaattatc agatttaata cataaagaag atatatttgt
    36421 ggatttacag gttaaatcaa aagaagaagt tctaaaatat atgagtaaaa aacttagaga
    36481 gaaaggttat gtgacaaaaa atttctataa aaaagtagtg gaaagagata acttatttcc
    36541 aactgaatta tcagatgaaa tagcaatacc tcacacagat ggaatagatg taattaggcc
    36601 agctataagt atagccatct taaagaagcc tattgtgtgg agtaaaagta aagtaaaaat
    36661 aatactttta ttagctgtag accaaaagtg ttttaatcca ttaagcactc tactctcatt
    36721 tatagaaaca gatgaattta agaagataaa aaacattaaa gatagagatt ttattaggga
    36781 gatgatatta gatggagtca atgaaaataa ttagtagcaa tttaattttt aaaaatattg
    36841 aagtgtctaa caatgaagat gcattaaagt ttttgggaca acgtttgttt gacgaacagt
    36901 atgtaaaaga aagctacata caagctgttg tagcaagaga aaaaaagtat gctacagggt
    36961 taccaacaga aatatacgga gtggcaatac cacatacaga tatagtacat gtaaatgagc
    37021 caggaatagc aattggaata ctgaataaac ctgtaaaatt tataatgatg ggtacagacg
    37081 atactgaaat tgatgtaaaa gttgtattta tgttagcagt gaaagagcca caagaacaat
    37141 tgcaactatt agaaaaactt atgactatat ttcaagataa gaatatacta aataatattg
    37201 ttgacttaag tgaagaaagt gtgagtgatt tattaaactc taaattgaaa aattaaacat
    37261 gtaaagtata ttaaaaatgt aaaattaaac atgcaaagta tgttaaaaaa tataaaaaga
    37321 ttattaaaga tttctaacta tagatatctt taaaataaaa taatttcaat gggggtgact
    37381 aatgtggcta aaaagatatt agttgcatgt ggaacaggag tatgtacttc aacaattgct
    37441 ataaacaaat taaagaaagc acttcaagac attggtaaat tggatatggt taatattaca
    37501 caatgtaaag tagtagaagt tgcatcaaaa gcacctgatt atgatttaat aatttgtact
    37561 acacaagtat ctagtagtat aaaaacacca gtgataaatg gattaccttt tttaactggt
    37621 gtcggaatgg ataaattaat caatgatgta ttagaagagt tagaattata attaaaaaaa
    37681 ttaaggaaca tggtctctaa aatcataagt tttagagact taaaattaaa taatggaggg
    37741 tctttatatg gagatattaa atttcattgt tggtctaggt gcacaagtaa tgatgccaat
    37801 aataatatgt atttttggtt tgatattagg tacaaaacta ggaaaatctt taagagctgg
    37861 tttaactgtg ggagttgggt ttataggtct taacactatt atagctttgc taacagataa
    37921 tttaggtcca gctacacaac aaatggttaa gaatttaggg ctaagtttat caataataga
    37981 tgtaggatgg ccagcagcat cagctatagc ttttgcatca acagtagggg ctttaattat
    38041 tccgataggt cttgtggtaa atatagttat gctaattact aatactactc aaactgttga
    38101 tgtagacata tgggattact ggcattttgc ttttacagga gctttagtgg caggggctac
    38161 tcaaagtgta atgtggggag tatttgcagc agtagcaaat atggtaattg tattggtaat
    38221 ggcagattta actgctccag gtatagagga atatttaggt atgccaggta tatctcttcc
    38281 tcatggattt acacaggcat ttgttccaat tgctatagta gtaaataaat tattagactt
    38341 aataccaggc ataaataaga ttgagataaa tgctgataca ttgcagaaaa aatttggatt
    38401 atttggagaa cctttaataa tggggtctgt aataggtgta ataattggta ttgctgcgaa
    38461 atatgatata aaaggaatat tacaattagg tgtaactatg ggagctgtat taatattaat
    38521 tccaaagatg gcagcattat taatggaagg attattacca gtatcagaag ctgcacagga
    38581 atttattgag aagagattta aaaacagagg caagatttat ataggcttag attcagcagt
    38641 aggaataggt catcctgtta cattatcagt tgcactagtg ttggttcctt tgactatact
    38701 tatagcagct atattgccag gaaataaagt tttaccattt gcagatttag cagtaattcc
    38761 atttgcactt gtattaatcg taccaattac aaagggaaat gtgtttagaa cattaataat
    38821 aggaataatt ataattactt ctggattatt aatcgcaact aacttagctc cattatttac
    38881 ccaaatggct ttaaatgcta gttttaaaat gcctgaagga gcaactatga tttcaagtat
    38941 ttgtgatggt gcgaatccat taagttgggt atttgtaaaa gttatgaact ataaagttat
    39001 tggaggagta gtttttggag ttatagcttt aggaatggct atttacaata ggaatagaat
    39061 aataaatgaa aataaaaagc tatctataga agaataaggt taaaacaatt ttattaagga
    39121 gtgtataggt tatgaaaaaa tataaatatg attctaaaag atacttactc agtgttacaa
    39181 taccaggatt ttttatgatt ttaatattga tttatgcact gtttaataac tatacaaatt
    39241 ttaatttgaa tatatatagt ttgttaataa ttgtctgtac ttatggtata ttcaatactt
    39301 ttatatcatc aagtaatcct aaaaaaatta ttattgatag caaatgtata cattttacct
    39361 catttatgac tacacataag tatgaaatta aagatataga aaagataaga ataagagaat
    39421 tttataataa aaaaatttat ctaaaaatta atgatggaaa cctttttaaa ggtaggtatt
    39481 ggataagaac aaatatgttt aataatagta tagcattata tagctatttc attgagttgg
    39541 aagagtgctt atatccagat tcactaaaat ttagaaataa aaaatttgag aataagaata
    39601 tataaaaata aaaaattgaa ttgaagccta gcttaatctt ttttgataag atataagttt
    39661 gaactttata ctaaattttt agaattttca atcaagacaa taacataata gctaagcaat
    39721 tagaacacaa ttaagatgta taaggcatgt atatgatgaa tgggaggatt taattaatgt
    39781 tattagagaa attaagaaaa gaagtattac aagcatcttt agacctttta aactataatt
    39841 tagtgacatt aacaggagga aatgtaagtg ggagagatga acaaactggc tatatagcaa
    39901 tcaccccaag tggaatggat tatagaaatc ttacaccaag tgatattgtt atagtagatg
    39961 tagatggaaa tattatagat ggaaaatgga aagcatcagt ggatttatca gaccacttgt
    40021 atatatataa gcatagagaa gacataaata gtataattca tacacattct acatactcaa
    40081 gttgttttgc tattttaaat gaacctatag aatgctcaag tacaactctt gcaaatgaag
    40141 tagggggttc agtaccagtt gcaaaatttt cgccaccaac atctaaaaag atgggaaaat
    40201 gtgttattga agcaattggt gacaaaagag catgtctttt agcaaatcat ggcgtaattg
    40261 cagttggccc atctgtagga catgctctta cagccgctgt aatgttagaa gattcagcaa
    40321 aagtatatta cttagcaaaa tcaattggca caccagtgct actgcctgat gaagaaattc
    40381 aaagagcgag agatgtattt tttaatgtct atggtcagga taaataaatt agataaaatt
    40441 ttaaaagtaa tatgaaaata tagatattca atgatattaa ggagatttat aatgatataa
    40501 taagtttaat agttaagaag taaaatatga aatgtaatag tatataaatt catttaagac
    40561 taaaaataat aagataaaat aaaaaggagt ctgtatttct atgtattact tattaaacaa
    40621 aagcacaaat ccttacttta atttggcatt agaagaatat ctatttttaa atgataaata
    40681 taatgatgat attataatca tatggagaaa tgaagaatcc atttttatag gtaaaaatca
    40741 aaatccatat caggaagtat atcatgatgt tattgaaaag ggagaaattc ctattttaag
    40801 aagaatatct ggaggaggaa ctgtttatca tgacttaggt aatatcaata tgtcattcat
    40861 acaaaaagat aggcaacttc atgagataga ttttttggag catactaagt ttatgcaaaa
    40921 gatgctttca accttaggac tagatgtatc tataactgaa agaaaagatt tatttttaaa
    40981 tgggaaaaaa atttcaggtt ctgcacagtc tataaaaaga aaaaattctc tttatcatgg
    41041 aactcttctt tacgattcag atttaaataa attaactaaa tatttaaatt caaataaagc
    41101 aactgaatca aatgcaacta aatctgtttc aagtaaagtt acaaatatta agtttttact
    41161 cgaaaaagat ataaattact ttttagatta ttgtgttgaa tatttaaaat ctaatataaa
    41221 gaatataaaa gaacttgaat tggatgaaga agatattaaa agtgtataca atataggaga
    41281 agataaatat aaaaagatag agtggacata tggtaaaacg ccgaaatttc aagtagtgct
    41341 accatttaat aatgaaagta aaataaatat acatgttaac agatggaaag tatcaaaatt
    41401 tt
//
LOCUS       DAGSHT010000014        40523 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGSHT010000014 DAGSHT010000000
VERSION     DAGSHT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.015"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(126..935)
                     /locus_tag="MJB72_000720"
                     /pseudo
     CDS             complement(126..935)
                     /locus_tag="MJB72_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1019..1552)
                     /locus_tag="MJB72_000721"
     CDS             complement(1019..1552)
                     /locus_tag="MJB72_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY8261846.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1702..3051)
                     /locus_tag="MJB72_000722"
     CDS             complement(1702..3051)
                     /locus_tag="MJB72_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBY8261847.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3063..4445)
                     /locus_tag="MJB72_000723"
     CDS             complement(3063..4445)
                     /locus_tag="MJB72_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBY8261848.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMIGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            4689..5903
                     /locus_tag="MJB72_000724"
     CDS             4689..5903
                     /locus_tag="MJB72_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBY8261849.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6080..7993
                     /locus_tag="MJB72_000725"
     CDS             6080..7993
                     /locus_tag="MJB72_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261850.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            8264..9238
                     /locus_tag="MJB72_000726"
     CDS             8264..9238
                     /locus_tag="MJB72_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBY8261851.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9259..9534
                     /locus_tag="MJB72_000727"
     CDS             9259..9534
                     /locus_tag="MJB72_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY8261852.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9547..10710
                     /locus_tag="MJB72_000728"
     CDS             9547..10710
                     /locus_tag="MJB72_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY8261853.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11047..13326
                     /locus_tag="MJB72_000729"
     CDS             11047..13326
                     /locus_tag="MJB72_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8261854.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSTGDTYFEINDSEKEVNKYLSKKLQLFEFDWLG
                     IIDSNGNFISALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYKGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSIYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(13421..14761)
                     /locus_tag="MJB72_000730"
     CDS             complement(13421..14761)
                     /locus_tag="MJB72_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8261855.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(14773..15555)
                     /locus_tag="MJB72_000731"
     CDS             complement(14773..15555)
                     /locus_tag="MJB72_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261856.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15570..16592)
                     /locus_tag="MJB72_000732"
     CDS             complement(15570..16592)
                     /locus_tag="MJB72_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY8261857.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16598..17611)
                     /locus_tag="MJB72_000733"
     CDS             complement(16598..17611)
                     /locus_tag="MJB72_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY8261858.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(17739..18677)
                     /locus_tag="MJB72_000734"
     CDS             complement(17739..18677)
                     /locus_tag="MJB72_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8261859.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            18947..19411
                     /locus_tag="MJB72_000735"
     CDS             18947..19411
                     /locus_tag="MJB72_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261860.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19523..19840)
                     /locus_tag="MJB72_000736"
     CDS             complement(19523..19840)
                     /locus_tag="MJB72_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY8261861.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(19853..20584)
                     /gene="chbG"
                     /locus_tag="MJB72_000737"
     CDS             complement(19853..20584)
                     /gene="chbG"
                     /locus_tag="MJB72_000737"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBY8261862.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(20610..21920)
                     /locus_tag="MJB72_000738"
     CDS             complement(20610..21920)
                     /locus_tag="MJB72_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBY8261863.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22017..23330)
                     /locus_tag="MJB72_000739"
     CDS             complement(22017..23330)
                     /locus_tag="MJB72_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8261864.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23352..23663)
                     /locus_tag="MJB72_000740"
     CDS             complement(23352..23663)
                     /locus_tag="MJB72_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8261865.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(23863..24567)
                     /locus_tag="MJB72_000741"
     CDS             complement(23863..24567)
                     /locus_tag="MJB72_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8261866.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(24658..26019)
                     /locus_tag="MJB72_000742"
     CDS             complement(24658..26019)
                     /locus_tag="MJB72_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8261867.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            26518..27672
                     /locus_tag="MJB72_000743"
     CDS             26518..27672
                     /locus_tag="MJB72_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8261868.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(27754..29472)
                     /locus_tag="MJB72_000744"
     CDS             complement(27754..29472)
                     /locus_tag="MJB72_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBY8261869.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(29477..30466)
                     /locus_tag="MJB72_000745"
     CDS             complement(29477..30466)
                     /locus_tag="MJB72_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8261870.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(30562..31611)
                     /locus_tag="MJB72_000746"
     CDS             complement(30562..31611)
                     /locus_tag="MJB72_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8261871.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(31888..32556)
                     /locus_tag="MJB72_000747"
     CDS             complement(31888..32556)
                     /locus_tag="MJB72_000747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBY8261872.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(32562..33935)
                     /locus_tag="MJB72_000748"
     CDS             complement(32562..33935)
                     /locus_tag="MJB72_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBY8261873.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(33932..35710)
                     /locus_tag="MJB72_000749"
     CDS             complement(33932..35710)
                     /locus_tag="MJB72_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBY8261874.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(35827..36150)
                     /locus_tag="MJB72_000750"
     CDS             complement(35827..36150)
                     /locus_tag="MJB72_000750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBY8261875.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(36137..37114)
                     /locus_tag="MJB72_000751"
     CDS             complement(36137..37114)
                     /locus_tag="MJB72_000751"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBY8261876.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(37129..37692)
                     /locus_tag="MJB72_000752"
     CDS             complement(37129..37692)
                     /locus_tag="MJB72_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBY8261877.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(37744..38235)
                     /locus_tag="MJB72_000753"
     CDS             complement(37744..38235)
                     /locus_tag="MJB72_000753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBY8261878.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(38238..40163)
                     /locus_tag="MJB72_000754"
     CDS             complement(38238..40163)
                     /locus_tag="MJB72_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBY8261879.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(40165..40476)
                     /locus_tag="MJB72_000755"
     CDS             complement(40165..40476)
                     /locus_tag="MJB72_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261880.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
BASE COUNT        13663 a         7012 c         5185 g        14663 t
ORIGIN      
        1 atagggtctc agaacttagt tacaagttct aaaaccctat ttgtttagtg taaaaaacat
       61 cataaaattt agcttaaatc ttttcacata atattactct ttttctaagt gataataaaa
      121 tatatctatt cctctaaaaa ctgtaaaaat tcctgattaa atagtttaag ctcatcatag
      181 aatatagcat gaccactata ctcaaaagta tataaaattg aatcttctat tttttcattc
      241 ataaaaactg caaattcata cggacatatt tcatctaatt tcccatgaaa tataccagta
      301 ggcactctta cacattttaa atcattaaat aattcttcat ctcttaaaga tacagcagtt
      361 ccaatagtgc caatacctga tgcactaaaa ccaatatcat taaaccatct tctaaagctt
      421 tctggtggat ttgatgcaaa tactttttcg ccaaaatcgg ttaccatttc tggtctatca
      481 gtacatgctt gtgctatcaa tttatttaca tcttcacgtg tcataccata tggaaattct
      541 ggaggtcttt gtacaaatga tggtgcagca gctgcagcta ataccaattt tgacactcca
      601 tatccattaa ataaactcat atacctaagt acgattgctc ctcccattga aaaaccaaca
      661 agggtaaagt cttttaatcc aatagcatgt accaccttat aaatatcgtc tgctaattag
      721 ctataagtat atcctcctga tgttgcatca gatttaccaa aacctcttaa atcaattgaa
      781 actgttctat atccgagttt tggcaatata tttgtttgat attcaaatat cttatgtcct
      841 aagggccaac catgtataaa aagtacagtt tttttagcac tcggattcaa atcatatact
      901 gctatgttta tatcattcac atatacataa tacatatcaa cagcaactcc cttcaagaaa
      961 atgtatgatg gttattttat atatatgaat aaaatgatga ataactaaat tttcaaattt
     1021 atttacaata cttctctatt gccttttcaa ataaacttac atgaagtttt tcatctagga
     1081 ttattctatt taatatagct tttatataag ggtcttcaat aagacttatg tctttataat
     1141 aattattaat agccacatgc tccaaatgca aatctagttt taatctgtta caaatagaat
     1201 ttccataaca tacaaaacca ccattccaat atgcaccaca agaacttgta gaacctctat
     1261 atacaggatt tccacccaat aatcttatag tctttgctaa tatctccata tgtaacattt
     1321 ctgttataga tatatttatc cacatttcac ttaactctct atgcgtttca tctaaatcaa
     1381 aattatggta tagatattga tttacactcg taaattcact tgatactccg gcatagtcat
     1441 ccatcaataa ttctgcatag tacttatttg gacctaaaac ttttatctct ggatatggct
     1501 catcacttga atatccattt cgcttgtgca tgtcataatg tgcatcactc ataatatctc
     1561 ctcctttaaa tttaatgtat gatattaatt gtatgcatca caactttttc atataactta
     1621 gataataaaa tctacttaaa taataaaaag ccatattaat taaaattaac atgacctttt
     1681 atccacaaaa ttacttttta attattcatc ttttccagct actgatagct ctttaataac
     1741 aactgaaggt gaacctatac tactcattgg aaattctaaa tcttctccta tagcttcaat
     1801 atctttcaac aggtcaaaat agtttcctgc tactgtaatt tgctccactg gaaatacttt
     1861 ttttccttct ttgatataaa agcctttggc agctaaagaa aagtctcctg ttactgaatt
     1921 tgcacctgaa tgaagtcctg caaagctagt aaccatcaat ccttcatcta tatcctttat
     1981 aatttcatct aaagatttat ctcctttctc tatataaaaa ttagttggat atatgcttat
     2041 aggtgatgaa tatgaagatt ttattccatt tcctgtagtc tttacacctg ccttattagc
     2101 cgtttttaag ttatgaagta aagttataag ctttccattt aaaacaactt cttttttaaa
     2161 tgtagctact ccttcatcat caaaaggagt agatgccata ccattttcta aaagtgggtc
     2221 atcaactatt gtaactatag gagatgctat catgtctcct tctctatttt tcaataaaga
     2281 taatcctttt tgagctgaat ctgcattaaa tatcccagaa aatgtgctaa gcaaagatac
     2341 catagcttca ttaagcaata tagttttata tttacctgat ggaatactct taccacctac
     2401 tttagataaa gcattatcaa caccaagttt tgctatctca caaggtttta tttcttcaat
     2461 agaatttgct atattatatc ctattccatc atatttttgt ccattgtctt ctatgatagg
     2521 aactacaaac cctataagca tattagtttt attgcttaaa ttaagacctt tagtattaga
     2581 tataccatta cttgatacag aataagatat agtacattta gataaattta ccaccttatc
     2641 tgaataagct ttagtctctc tttccatttc taaagctaaa tcaatcaact tatctgcctc
     2701 taaattttct agctctttag aataagtctt tacatcatta tagtgcttat ctccttcata
     2761 tataaattgt acatcttcat tttcaatagt gtttactcca tcttttacat tttttatcaa
     2821 catatcaaca gctttatcat ctaaaatttc tgtataagaa taacctatct ttccatttac
     2881 cttacctcta aaagataaac caaaagattt atcaagattg tatttctcaa cttctccttc
     2941 atatatattt atacttaaat tttctcctgt ataataatat atttcacact cttcaaaacc
     3001 ttcacttaga gcctttttaa ataatatatc tttaaaattt ttaagttcca caattatccc
     3061 tcctatcttc cacctacagt aatttctttt acccttatca taggttggcc tacattagtt
     3121 ggtatacttc ctgatgatga gccacacatc ccctgagctt gttttacgtt attcccaatc
     3181 atatctatat tcataagtat atcgctacct tttccaatta aactagcacc tctaactggc
     3241 tcttgtattt ctccattttt tattaagtaa ccttctgaaa ctgcaaaatt aaactctcct
     3301 gttacaggat ttacagaacc accacccatt tttttagcat aaagtccatc tggtattgac
     3361 tttataatat cttctggttt atcctctcca gcagcaatat aagtgttagt catccttgaa
     3421 gttggtgcaa atttgtagtt ctgtctacga gaacttcctg taggattcat tcccattcgt
     3481 ctaccattaa gtttatctat catataagac ttcaaaattc cattttcaat cagtatattc
     3541 ttttgagttg gatttccttc atcatctata ttcatagaac cccagtagtt tggtattgta
     3601 ccatcatcta tagctgtaat ctttgtggaa gcaatctgtt gacctaattt atctgcaaat
     3661 acagagttcc cctttgcaac tgatgtagct tccaaagaat gtccacaagc ttcatgaaat
     3721 attactccac caaatccatt atctattgct accatcatat tacctgcagg acaattttta
     3781 gcatgcaaca tagtatgtgc tactctagct gattcttttg cataatattc tgggtctatt
     3841 tcttcaaata tttcaaaccc tttacaacct ccaggacctt caaatccagt ttgattttca
     3901 ttacccttag atgcaattga acttatccct agtctagttc ttattcttct atcctcaaca
     3961 tatatacctt cagtgtttgc aattaatatt ttttggtctt tatccaaata actaacactt
     4021 acttgagata tatcattact aaattctttt gcactcttat atgcaacctt cataacactt
     4081 actttatctt tatttcctat tgaatttgga taaattttta tattatgtat gttatttatt
     4141 ttaatactgt catttaatat tacacttttt ccatcttcca atttacctaa agcaacagca
     4201 gctttgtatg ctacatccaa cagactattt aatgaattgt cacttgtata agcatatata
     4261 cttttcaatc ctttaaatat tcttattcct ataccataat ttcttccacc tattgcattt
     4321 tctactttat tatctattaa actaatagaa ttatttatag tgtcttcttc aaaaatctca
     4381 gcaaaatctc ctccagtaat tagacacttt tctaatacct tagaagctat attttttgaa
     4441 agcataaatt tacctcctac tttaaatttt taattaactt ttattcaatc tttttacttg
     4501 ttaacaaaca ttatatacta tattacttaa aataaggtaa ttatgaatat attatacata
     4561 tgttttatac taaaatgtta tcacatttta tctatacata cccatataac tagataatta
     4621 aaatgtataa tattatttgc tatagaaaat aatacactca tacataataa attttaggag
     4681 tgaagtatat gaatcaagaa tatgtaaaag gaaatcctat ttttactaat attaataaag
     4741 ttccaagaca atttccatac cttactgatg atattgatac tgatgttata attgttggtg
     4801 gtggcgtaac tggatgtatc tgtgcttact atctagctaa aaacaatata aagtctgtca
     4861 ttcttgaaaa aggaaggatt gcacatggaa gcactagcgt gacaacttct ctattacagt
     4921 atgaattaga tgacaatttg atagatttaa cagaggttat gactctaaaa gatgctctaa
     4981 aagcatataa cctttgtact tcagcacttg aagaattaga tacatttata gagctatatg
     5041 gaaataagtg tgattatgcc aaaagagata ctctacttta tactgccaac aaacttgaag
     5101 ttaaggctat caaagaagaa tataacttaa gaaaagaaaa tggatttgat gttgaatata
     5161 ttgatgaatc cacaaaccct tttagctttg atttaaaatc aggattgata gcaaagaatg
     5221 gaggacgaga attagaccca tataaatata gtcaccattt gattgatgta agtttaaaaa
     5281 atggtcttca agtatatgaa aatacagaag taaaaaaagt agatttatca aatgataagg
     5341 ttactgctga agtatcgtat ggatacaaag ttcatggtaa aaaattaatt gtagctacag
     5401 ggtacaatac aagtttattt acaaaaagaa attttgcaac taagtcaaat acttttaata
     5461 tcgcaacgaa accacttaaa aacatagctt cttggaaaaa caacatttta attagagata
     5521 actgtgaccc atataattat ctaagaacta ctaaagacaa tcgattaatt ataggtggtg
     5581 aagatgtaag ttttgatgat atagaaaatg aaactttagc caatgaaaag tacgatattt
     5641 tagaacaaag attgaaaagt atgtttaagg atataaaaga cattgaagtt gaatataaat
     5701 attgtggttg ttttgcttca actttagata atttaggttt tattggtcca gataataaaa
     5761 ataacaatct atggtattgc ttaggttatg gagcaaatgg tatactcttt gcaatattag
     5821 gaggtataat gttaagtgaa ttatatcttg gcaaacaaaa taaaaatatg aaattattta
     5881 aagtagatag atttgataaa tagatattat atattaaaag actgtaaaaa gatgaaactt
     5941 tataattcta aaagttaact ttgatacagt cttttataat gttcctttta aaaatacttt
     6001 atttataatt aaatataaag taaaatgtta aaatataact aataaaattt agtcataaga
     6061 taatatttaa gtttatttga tgaaatatgg aggaattatt atgaaggaaa tactcgttat
     6121 tgctcccact aaaggaacct atgaaaaatc aattcacata gttaaaaaaa acaaatatac
     6181 aaatattgat gttgtatttg gcaatctaaa agaaggtata cctcttgctg aaaaaagtat
     6241 taatcatggt actagaatta tcataagccg aggtggaact tacaatatgt taaaagctac
     6301 ttacaatata cctatagttg aaattaaggt agatgcttat gatattataa aaagctacaa
     6361 agaggtaaaa aattccaacg aaccatttgg aataattgga tttaataatg tcatctacgg
     6421 tttcgatatt atagaagaaa tattgaataa gaaaataact atgattgaaa ttgaaaaaga
     6481 agaagaaatt tatgatgcta tagaaaagta cagaaagaaa ggtataaaca catatatagg
     6541 agataccact gttgcacata ttgttaagag acttaattgt aagggaatat taatcgaatc
     6601 tagagaagaa aacatactta gagccattca acaagcagag caaatactcg aagcaaccaa
     6661 ggatgaacaa aaaagaagat tacagataga agctatgaca gattttgtac atgatggaat
     6721 aataacagtt gataaaaatt ttaaaataac attatttaat aagagtgctg aaaaaatatt
     6781 tggtataaaa aaagaaaatg cactttatca taatgttgta gatgttatcc caaacacagt
     6841 tcttccacaa gtagttgaga ctatggaacc tcaaattggt gaaatacaaa atatagggag
     6901 tacaaaaatt attacaaata gagttccaat cattgttgat ggtcagattc aaggagctgt
     6961 ttcaacattt caagatagca aggagataag ttcttttgaa catacaataa gaaggagctt
     7021 attaagcaaa ggtctatctg ctaaatacac ttttgatgat ataattcatg ctagtgaaaa
     7081 gacaaaagat tgtatagaac ttgcaaaaaa atatgcttgt tatgatactc ctatgattat
     7141 tactggagaa tctggagttg gtaaagaact attttgtcaa agtatacata actatagtaa
     7201 gcgtaaaaat gcaccttttg ttgctgtaaa ttgtgctgcc atacctccat ctctaataga
     7261 gagtgagttt tttggttatg aggaaggttc atttacaggt gctagaaaaa aaggtaaagc
     7321 aggtgtattc gaactagcac atgaaggcac aatcttccta gatgaaataa gtgaaatccc
     7381 tcttgaactt caaggtcgat tattgagggt attgcaggaa aaacaagtta tgagaattgg
     7441 tggtgataaa gttattccta tagatgtaaa aattatctgt gcttcaaata aagacttaaa
     7501 aaatatgatg cgcaatggtt tatttagaca agacctttat tttagaataa atatactaac
     7561 attatatctt ccttcattaa aagaaagaaa agaagatatt ataaaattat cagaattttt
     7621 tatacataaa tattcaagca aatacaataa aacgccttta attatcactc caaatattaa
     7681 aaaggcactt cttgaacgag aatatgaagg taatataagg gaattggaag gtatgatgga
     7741 aagggctgtt attgtggaat catttgactc gattttatta gaaaagctta gcaatgaata
     7801 tacaaataat atagttgata aagataatag tgattttgaa tgcagtaatt tagatttaaa
     7861 aactttagaa aaaaaatata tctataaaat ttataacgaa aataatagaa atactacaaa
     7921 aacctgtgaa attctaaaga taactcgctc cactctatgg cgtaagttaa aagaaataga
     7981 agatgatgtt taaaaataat acatatctat atatatcaaa acgttttatg tttaatttta
     8041 atacataaga cgtttatttg ttttcaataa aaaatacaat tatatgataa aaagaatctt
     8101 tgtaagtgaa attctcgtca atcatacttt taattataag taatctacag catttcaatc
     8161 gattattaaa aataatcata ataactaaag atacaaactc tatatattgg catagtaatt
     8221 gcttttataa tacctatata aaaatgaaag ggagtgatac tagatgcaca tcttaaaatc
     8281 agtgaaaaag ataccaggag gacttatggt ctgtcccttg ttatttggag cactatttaa
     8341 tacagtatgt ccacaagctc ttgaaattgg aggattcaca acatccttat ttaaaactgg
     8401 tgcaatgagt atacttgctc tattttgtct atgcaatgga gctcaaatca atatcagaca
     8461 agctggtaaa ccattgacta aaggtatagt acttacagca acaaaattta taattggtgc
     8521 agtattaggt attattgtta gtaaatttat tggacctaaa ggtataattg gtattacacc
     8581 tcttgccata gttgctacca tgactaattc aaatggaggt ctatttgctg ctcttgcagg
     8641 tgaatatgga gactccacag atgttggagc aatatctatc ttatctctaa atgatggtcc
     8701 tttttttaca atgcttgctt ttggagtaac tggacttgca aatgtaccta tagtagcact
     8761 atttgcagca cttataccaa tactacttgg atttatactt ggtaaccttg atgaagatat
     8821 acgagaattt cttgcacctg gtacaacatt attaattcct ttcttcgctt ttcctttagg
     8881 agcagcatta aactttaatc aaattataac tgctggacta cctggagttg tacttggttt
     8941 aggagttact ataataacag gtatgggtgg gtactttgta atgaaactta tacgtgcaga
     9001 acacccagca gtaggtgcag ctacaggtac aacagccgga aatgcagctg gtactcctga
     9061 agctttagca gctatagacc ctactctagc tacaatagca gctgtttcaa cagtacaagt
     9121 tgctgctgct attattgtta cagcaatatg ttgtccaatg ttagtttcct atctagataa
     9181 aagagaaaaa aagaaaaaat ctatttttaa aacaaataaa aatattgaag taaattaata
     9241 cttaaggagg taaaattaat gaatgcaatt attcatgcaa atgaaaaaga taatctagtt
     9301 acatgtgtca gacctttaat taaaggtgaa aaagtttcaa ttgatggaaa gtcttacata
     9361 gtaaatgata atatacctgt ctttcataag atggctacag aagatgtaaa aaaaggtgat
     9421 gttgtataca aatacggtga aattatagga attgcaacag ttgacataaa aactggtgac
     9481 tatgttcatg tacataacat tgaaagtaca cgtggtcgtg gagataaaaa ataggaggaa
     9541 gttaaaatga aattaatggg ttatttaaga gaaaatggtc agtttggtat tcgtaatcat
     9601 gttttaatta ttccaacatc tgtatgttca agtgagacag ctacaagaat agcatcactt
     9661 gtgcctggag caatagcaat ccctcatcaa catggatgtt gtcaaattgg ttctgatata
     9721 gaacttacag caaagacatt aataggattt ggaaaaaatc ctaatgttgc cgcagtttta
     9781 gtagttggtc ttggatgtga tggtattcaa gcaaaagaat tagcatctga aatagctaca
     9841 acaggaaaaa aggtagatta cgttgttata caagaatgtg gtggaacatt aaaaacagtt
     9901 tctaagggag ctgaaatagt aagtaaaatg gctcgtgaag tatcaaaaga agtaagagtt
     9961 gaatttggta tgagtgaaat tacattagca cttgaatgtg gtggttctga cccaacaagt
    10021 ggaatagctt caaacccttc aataggcgtt gcatcaaacc ttcttgtaga tgaaggtgga
    10081 agtagcatac taagtgaaac tacagaagta attggtgcag aacatttgtt ggctacccgt
    10141 tttgaagatg aagaaatgaa agataagttt cttaaatttg taagtgatgt agaaaaaaga
    10201 gcaattgcta tgggagaaga tttacgtagt ggtcaaccta ctccaggcaa taaagctggt
    10261 ggactttcaa caattgaaga aaagtcactt gggtgtatgt ataaggcagg caataagcca
    10321 ttcaaaggtg ctcttgaata tgctgatatt gtacctcctg ataaaaaagg attatatttt
    10381 atggatactc caggtcaaga tatagattct atcactggta tggtagcagg tggtgcacaa
    10441 attgtaatct tttcaactgg tagaggtact ccaacaggaa gccctatttc accagttatt
    10501 aaaatcactg ggaatagtga tacatacaac aaaatgccag acaatatcga tataaatgca
    10561 ggtagaatta taacagatgg tgctaaaata gcagatatag gtcaagaaat atttaatgaa
    10621 ataatagaag tctgtaatgg taaacatacc aaagctgaaa gtcttggtca tagagaattt
    10681 ggcatttaca gaatttcatc tacattttag attgtaacaa aatatctttt ataaatataa
    10741 tacttatgta gatacataag ctaaaagttc tttctaatcc tataaaaatt ttctatactg
    10801 aaaaggaact gtatcaaaat tattttgaaa atagttcctt ttttcacctc ttttactcaa
    10861 aatattataa aataagattt aaacaataaa cgtatcttga ttaatctcta agaagacata
    10921 aataaagcta aatgtaaagt gagataaata aaatttaata tctttgtatg aaaagatatt
    10981 tggtataata tgtctgagag aattcttatt tgtaaacaaa taaaggatgg aggatgaaac
    11041 aaaagaatga actttgaaaa cacaaaaaat aaaaaaataa tagctacttt actattgaca
    11101 ctccttttaa tcgtgtcatt aacatgtgtc acttttatta tgaatctaaa taaagtaaca
    11161 cataatgaaa cagaacgata tcttgtagaa gtttcacaaa gtatttccac tacaattaat
    11221 gtgaaatgta aaagtatatt taggacacta tattctacgg gagatactta ttttgagatt
    11281 aatgatagtg aaaaagaagt caataaatac ttatcaaaaa aattacaatt atttgaattt
    11341 gattggttag gtattataga ttcaaatgga aattttataa gtgcccttaa agatacaggt
    11401 aatgttaaag gacaagactt ttatagaaaa gcccttcaag gtaaagaaac aattactcct
    11461 gttaaatcac aatctggtac atatggaatt ttatattctg taccactata caaaggtgat
    11521 aaaattgttg gagctgttgc tgcttggaat actattgata ctatgagaaa tttattaagt
    11581 gtagaaagct ttcatggtga aggattttcc caaattatcg accaagatgg aaattttatt
    11641 gtaagttcag caaacaaaaa tgctccaaaa aatgtggtga atttttttga catgattaaa
    11701 caccaaggaa aacaggttga taaaaataaa cttgaagaaa tgaaaataaa cttaaaaaag
    11761 aacaaaactg gttctatata ttacacatta aatgatggta tttctaaggc aatgcactat
    11821 cttccattac aagaagaaaa tttatacctc ctttcagttg ttccaacaaa aacagcaagt
    11881 gcacagtttg atttacttct aaaaaaagca atcttcatca atattttaat tgttatacta
    11941 tttttattgc ttattatatt gattatgttt ataaatcaaa aaaacagggc acaacttata
    12001 aaaatcgcct atgtagactc tgtaactaaa ggttacagta aagcacgttt tgatatggaa
    12061 gctaaaaaaa ttatacagtc atcttcatca gaaagctatt cattagtatc tataaatatt
    12121 cagaaattca aacttattaa tgattctttt ggtagtgaag caggagataa aacattaaag
    12181 tacatacatg atgtcattag taattatcta gaaccaggag aattactttg tcgagtttct
    12241 aatgaccagt ttaatgtact tattaaaact attcctcaaa aagaaattct taagtacata
    12301 gaaaatatca ccaatgatat taatagtttc aataaaaatg aagaatgtaa atactttata
    12361 gctctatcta taggtgtata taaaattgat gacccaaatc tacctttagt ttcaattcaa
    12421 gaccgtgcaa atgttgcacg aaaaaataat aaaggaaaca ttggaaatca tccactttat
    12481 acatgtgttt tttatagtga tttagagcgt ttaaaaatga ttaaagaaaa agaaatggaa
    12541 aatagaatgg aggaagcatt aaataataac gaatttatag tatatcttca gcctaaaata
    12601 agcctcaaag ataatactat tgtaggtgca gaagcattag taagatggca agacccaaat
    12661 aaaggtctta taccaccaga cgagtttatt cctttctttg aaaaaaatgg ttttattaca
    12721 aaactcgacc actatgtttt tgaaaaagta tgcagtatga taagaaaatg gatagataat
    12781 ggagaaactc caatacctgt ttcagttaat ttttcacgtg ctaatttaaa taatatagat
    12841 tttttagaaa aatacagaat gcttcgtaat aaatacaaca ttcctgctaa gctcttagaa
    12901 attgaattaa ctgaaacact tgtatttgaa aatctccaaa aattaattaa tataatagac
    12961 caaatccatg aagaaggttt tcaatgttca ctagatgact ttggaagtgg atactcttca
    13021 ctcaatatgt taaaagaaat aaaagttgac acattaaaac ttgaccgtgc tttttttagc
    13081 tcacctaatg cagataatat tgctgaaaat catgtaatag aatctatagt taatttggca
    13141 aaaaaattaa atatgaatag tatatcagaa ggtgttgaaa caatcttgca aatggattat
    13201 cttaaaaaaa ttaattgtga tatgatacaa ggatatgttt tttcaaaacc tctacctcaa
    13261 gaaatatttg agaaaatgac ttttggaaaa aaactagaag aaaccagtat ggatagttcc
    13321 gtttaaataa aataaaaaac ttgtctctat aaaataattt taatcatttt ataaagacaa
    13381 gtctttttat tttccaattt aattttattt tctaaatttc ttacactcgg cttgttttaa
    13441 attccctttt taagaataat aatacgatta tcatagcaat aaaatctgca actggctgtg
    13501 ccaaccaaac accatctaaa ttaaattgaa ttggaagtac aatgattaat ggtataaata
    13561 aaataaattg tcttaaaaga gttaaaaaca ttgaatgttt aactttactt atacactgaa
    13621 aatacacagg tcctacaata gaaatcccca ttataggcaa tgtaattaga tttaggctta
    13681 tacctcttac tgctatatcc atcaaatctg aatctttatt aaatatacct acaaatactt
    13741 ctgggaaaac ttgtactact aaaaatccaa atgacaatat gacaactgac actatgatag
    13801 ataatattaa ggttttcttt gcacgctcat attgttttgc accataattg tatgatatta
    13861 tagtttgcat accttgattc aatccaaaca caggcatcat aaacatcaat aaaatagatg
    13921 taagtgctgt catagctcca atagctaaat ctccaccata aacctttaga gaattatttg
    13981 taaacatgtg agttatactt atagctactt ccatagcaaa aggtgtcatt cctattgcaa
    14041 ataccttctt tactatatct ttattcaatt ttaagttaca tttttttagt tttaaattgg
    14101 acttccctct tataaaataa tgtattaccc aaaaaaatac tactatttga cagagtactg
    14161 ttgctatagc tgctcctttt atacctaaat taaatacaaa tataaatata gggtccaata
    14221 tcaggtttaa tacacatcct acaatcattg tccttgctgc taattttgga ttaccttctg
    14281 ccctaattgc attattaaga gaaaatgcaa ctaaattaaa tatacttcca accaatatga
    14341 cactcatata gtcttttgca taaaatattg aatctttact tgcacccaaa ataaaaagta
    14401 tctcttctaa gaaaactaat cctaatatag ttataattaa agagataatt atagataatg
    14461 tcaaggcatt acctaatatc ttctctgctt cttctctatt tctttcccct aactttatag
    14521 acaatcttgt gctagcacct actgatacca acattccaaa agctattatt agagtaaata
    14581 ctggcatagt tacaccaaga cctgcaatag ctatagagcc aataccttcc atagaaccaa
    14641 taaaagctct atctactaca ttataaagtg aagttaccat cattgctatt attgcaggta
    14701 tcgaatacct aaataataaa atccatattt tttcatgtct taatgcttct tgattctcca
    14761 tgttgtcacc atttacacca tgtcataagt tatacaaatt ggtttatttg ttcttgggtc
    14821 tgttgctatt tctgcatcaa tattaaataa ttcttttaga ttatccttcg tcataacatc
    14881 atatgcatca ccttcacaaa ctattttacc tttcttaata cctatcatgt ggtcagcaaa
    14941 tcttgctgca ttattaagtt catgtattac catgacaata gttcttcctt gttttttatt
    15001 tagttcatct aataatttta gtatctccaa ttgatgtgcc aaatctaaat atgttgtagg
    15061 ctcatccagt aataatatat cagtttcttg tgccaaagcc atagctatcc atgccctttg
    15121 tctttgtcct cctgataaat cttccatatt tctatcctta aattctgcaa ttccagtaac
    15181 ttctaaactc caatttacaa tctctctgtc ttctttacta gaattaccaa atccactttt
    15241 atgaggaaat cttccataag aaattaattc tgaaacggta agaccactag gagcttgagg
    15301 gctttgtgga agtacagcca tatcttttgc tatttcttta gacttctttt tgtagatgtc
    15361 ctctccattt atatatattt tcccactatt agggctaata attctaccta tagttttaag
    15421 tattgtcgat tttccacaac cattagcccc tatgatggta gttatttttc cttttggtat
    15481 atttaaattt aaatttttta taatatctgt gtttccatat gatacattta gattcttagt
    15541 atttatacag ttcataacaa caccctttcc taatcattta ccaacattaa ataaataaag
    15601 tacggcactc ctattataga aactactatg cctactggta tttctatagg tgctagaaga
    15661 tttcttgata tagtatctgc tattaaaagt aataatgttc ctatcaatgc tgatactggt
    15721 attaatcttt tatgctttgg tcctattatt tttctaccta tatgtggagc tattaaacct
    15781 aaaaatgaaa tactacctgc tactgctgtc gatacaccag ataaaattac tgcatatatt
    15841 ataagtcttc ttctttgttt ttctacatct acaccaagac ttatagatac ttcatcaccc
    15901 aaatttaaaa catctatata tttagattta taaatagtta tacccataaa aatcactata
    15961 aatggaagca cagctattac atatttccaa ctggttcccc aaatgcttcc tgatgtccat
    16021 gccataaccc tattaaattc ttgtgttgta aatttcaatt ggaatatcgt caaaattgaa
    16081 gtaaaagcta tgtttacacc tataccaata agtagtaatc ttgacgaatt tataccacct
    16141 ttccatgcaa gagaatatat aagaaatgct ccaaataaag ctcctataag tgcaacaata
    16201 ggcattgtaa atacagtcaa gttactcata ccttcatata cattaccatt cataaggtat
    16261 atataaacta ctacaaacaa tgcagcacct gaatttattc ccaaaattcc agaatcagct
    16321 aaatcattct ttgtaactcc ttgaagtatt acacctgctg ttgataatgc agaaccaacc
    16381 aaaagtgcta ttactattct tggtaatctt aatttaaata ttgcaatttc atgattacgc
    16441 tgaccctgcc ctaaaaaagt ctttataaca tccattggtt ctatagaaaa tgagcccata
    16501 tttaaactca ataaaaaagt gataaaaata agcaccatta aaattgatat aacaagtaat
    16561 gttttagaat ttatttttat aatatttttc atagtaccta tttctccccc tttctaacaa
    16621 ggtatataaa tattggcact cctataagtg ctgttataga acctactggt gtctcatatg
    16681 gaggatttat catcctactt aatatatctg tatatacaag caatacagct cctaatacta
    16741 aagagctcgg tattatatac ttataatctg aacctactat tcctttagct atttgaggaa
    16801 ctataagacc tataaaaata atattaccag acactgacac agatgctcca gtcattaata
    16861 ttacaagtac tatacatata aatctaacaa agttagtttt ttgtcctaat cctattgcca
    16921 cttcttctcc aagacttaat atagttattc ttggagccat aataatagct aaaataaatc
    16981 caatacctcc agctataaat agcaatttta taccttccca cttcactcct acaagccctc
    17041 ctgatatcca gaaactcaac tgttgagata ggttaaaata catagatatt gctgatgcca
    17101 aagaaattaa caatgtacca agagctactc ctgcaagtgc tagttttaca ttattaactc
    17161 ttcctcttga tttagaactt ataaaatata taaataatcc acttatactt gccccaataa
    17221 atgcaaacat cattacacta aaacttccat gtataagttg aggaagtttt ttttgaagta
    17281 ccagtgatat cgaaattgca aatgtagccc cttgtgtaat tcctataaca gatggttcag
    17341 ctataggatt tctcgttatt ccttgcatta ttgccccgga aactgctaaa aaacctccaa
    17401 caagtgcagc tgctattgcc cttggaagtc taacatcctt tactattctc atatttattc
    17461 catcttccat ttttatcaag ctatctataa cagttgatag attcatatct tttgccccaa
    17521 tagttatgga taatacaatt ccaaatacaa gtacaactat acttactaat atcaacaaat
    17581 aagttatatt ttttttactt aacttagtca attatcctac ctccttttcc cttttaatcg
    17641 tcagtataat atcatctatt aagtatttta attttaagct ttattaaaaa taggtgtgaa
    17701 aaatgaatat cattatctca cacctactaa gttaagaatt actttgttaa ttcattttta
    17761 actgattcta ataataactc ttttccaatt ggattgtatg attgactgaa aaatggagca
    17821 gcgtcaagta ttgttacatt gccatctttt actgctctta tttgagccca gatagcacta
    17881 ttttctaaat cttttttatc tgattctgta gcaattacaa ctatatggtc tgcatctata
    17941 tctgttaaac cttccatact taccattggt agtgttattc catcctgttt tggcatattc
    18001 ttaggtctag ctaatttcat atcatcattg attagagtac ctattccacc atcgctaaat
    18061 accataaatt gacctgaact tgccaacact ggtaaataag ttttttctcc attcttttct
    18121 ataacttcct tacctaactt tgtagctttt tcatcatact tctgtagcca acttttagct
    18181 tcttcttctt tgtcaaataa ctttgaaaca tcagttaact tgcttctcca gtcatttgca
    18241 taatctttca tcataactac aggagctatt tcttttaatt ggtcatatat tttttcttgt
    18301 ctttgactca ttattattaa atcaggattt acttctaata tagcttccat atccatagta
    18361 tccatcattg agtgcccaac tatctttaca cctttaagct cttctcttat gtaacttggt
    18421 aatttatcag tttcatatga gtctacattt gctgttgcaa caggcttata acctgctaat
    18481 aatagttctt cactacttcc tgatatatca actatttttt taggatttga tggtatctta
    18541 acctctcctt taacagattg tacaactctt gtctctgatt tatctttatc ctctgtattc
    18601 ttatctgaac atgctgtaac aagtactaat gtagttataa tagatataaa tattgcaagt
    18661 gattttattt ttttcatgat tacttctcct acttgtttat tatacttaca tgatattgat
    18721 tatcataagc acatatttat tatagcattt ttctattttt agtcaatatt attttgtaaa
    18781 aggataaaaa ttcctaatta atcttaatta tatatagctt agataaggtt ttcttatatt
    18841 aaacagtatt tttttaaaga attggacaca aatacaatga cttttaggtt aaacaatgat
    18901 aaaatgcaat ttatagataa tgaatttagt aaggaggatt caaaacatgg tacatttagt
    18961 atattgtgat aatgcaggaa aaaaaggaga aaaggtctta gataaaatct tggctggaac
    19021 aaaaacaatg gttattagag gtgctgctgg tagaaaaata ccacatagta gagtatttga
    19081 agatgagtta ctttatttta tggaaaaagg aactgcaaaa atatctgcaa aagccactgt
    19141 taaaaaggta gaaaactatg ttaaattaac agaagatgaa atttctaaaa ttttaaatga
    19201 aaatcaacca aaattaaatt tatctgaaaa gcaaaaagtt cgttggcata aaaaatgttt
    19261 atgcttagtt gaatttgaaa atgttgaaga aataacacct cttgattttg accatcaagg
    19321 aaatatggat gattggctta ttgttgaaaa aattgaagat gtagttgttg gtactagtat
    19381 accttacaat tatgagaagt ctaaatttta aagtatcaat aacataatac tatattgcta
    19441 aactctactt aaataaaaaa agagtgcatt taataattat aaaaattatt tttatacact
    19501 cttttttaat aataatttta aattacttac tttctaatct ttcatataca tctataaatt
    19561 cttctgctaa ttgttgaaaa ttcatagctg tcattaaatg gtcttgtgca tgaataagta
    19621 taacacttat ttcagtttta ttaccattag cttcattttg tatcaattct gtttggaatt
    19681 tatgagcttt atgaattgat tctttcgact tttcaataca ttctctagct ttatctatat
    19741 tcccgctctt tgcttctttt attgcttcaa atgctagccc tttcgcatca cctgcaaatc
    19801 caattattcc aaaagatatc tctatcatct tctcatccat ttattcgacc tcctaaatat
    19861 ctctatagct tgatatgact aatccattct tttctaaaaa ttctttgact tttttagatg
    19921 taagtatctt gtgctctttc gttctatcaa tagcataaga tgtagaattt aaaatataat
    19981 catctaaaaa agctgggtga gacataatat ccactacatc atacttcatt atttcgtcta
    20041 tattttttat aaaatattct tcacttacat tttccttata aaaactatct attaaaggaa
    20101 ctactttaga atactctaaa ttatattcaa atccacctct tattggaaga ttatacttgg
    20161 ttacaatctt ctctattact ggctttaaac ttactaaagt atggatatga tggtgactat
    20221 ctaaatgaga tatatctatt ccaagccctt ttaccctatc tatttgagca caaagttctc
    20281 tataaatctc atcacaatcc attttttcaa taatttcgtc tgtaatcctc ctaataaaaa
    20341 aaccgtcttt atcaacaata gtttttaagt tagataataa tggtctacca caacttaaag
    20401 tcatatgaac tccacaattt aaagttttat tttctttaag taagcctact ccatgttcta
    20461 ccccaggcat atttgccatc atagaagtag acctaactat accattatta tgagctgaaa
    20521 ttattccata gttcacagct tcacaatatc caaaatcatc tgcatttatt ataattttag
    20581 tcataccata acctactttc tatagacaac tatctattaa atctaggaag ataatcttta
    20641 tgagcttcca acatttcgtc taatatagtt tttgctaagt catcagatgg aatcaatggg
    20701 tttatacaaa gtgccaaaag agcagattca taatcgccat atacagctgc tttagctgcc
    20761 aatacttcaa atgatttaat ttgacttact aatccatgca cagaatctgg taaatatcct
    20821 atacttattg gttttggacc attttttgtt attacactac ttacttctac cgcttgttca
    20881 tctttaaaat ctcttataga acccttattt aatgtattaa ctacttggat atcttttttg
    20941 tcgttgtata tagaacttat taaattacaa gcagcatcac tatagtaagc ccctcctctt
    21001 ttttcaagtt gaggtggttt tacatctaat ttttcatcct tatataattc aaataattgt
    21061 tcttccaatt ctttaactac ttgacctcta gcttttccct ctttaaactc tctcaactca
    21121 tcctcaagca tttctttagt tttatagtag tatctatgat atggacaagg tattgcaccc
    21181 aaagatttta caaattcagc attaaaatca attgccttta tattttgcat acttatatct
    21241 gctttgacaa atttatcaat tgcttctttt gtaacatctt ctccatctaa agatacattt
    21301 agaccataaa ccatatgatt aagacctgca aaatccatac ttattctgtc actttctaca
    21361 ttaaatagtt tggcaacatc attttttaaa tgaattggca cgttgcatag acctatatat
    21421 cttctaaaat tagtatattt gaatacagcc tctgttatta ttcctgttgg atttgtaaaa
    21481 ttcaccatcc atgcatttgg acataattct tccacatctt ttattatatc aaatataact
    21541 ggtattgtgc gaagggcttt aaataaacct ccagcaccat tagtttcctg ccccataact
    21601 ccatgagata aaggaatagt ttcatctttt attctcgcat ctaaaagacc tactctaagt
    21661 tgtgttgtca caaagtctgc atctcttaga gcttctcttc tatctaaagt aagatttatt
    21721 ttcatatcta ctccagcttt ttttaccatc ctcttagcta gatttccaac tatctctaat
    21781 ttttcttttc cagcttctat atctacaagc cataattcct ttacaggtaa ctcttcatat
    21841 ctatttataa aaccctctac aagctcagga gtataacttg aacctcctcc aattgtaact
    21901 atttttagtc cctttttcat ctcattatac catccttacc tataatttaa tatactctac
    21961 aaaaccccat acatttattt aaaactctat acatttattt accgaaataa aactacctag
    22021 ttgttaattt tcaatttatt agcttccatt ttttcagacg ctatgacaaa tggaatatat
    22081 attaatattg ataatattaa attaaatagt gctaaagctc caccagtcca gtcacctgta
    22141 gccattgctc cacctaatat aggaggtgtt acccaaggta ttttaggtaa aattactggt
    22201 gctactaatc caaaatctat tgctaagtat gatacagcac tacatattac tggtgcaagt
    22261 acaaatggaa tcataaatat aggatttaaa actattggaa gtccaaatat caatggttct
    22321 cctatgttaa atattgcagg tggaccacct aatactatca tctctttacg tcttcttcca
    22381 gctattaata aagctactat taatcccaat accattccag aaccacctaa atatacaaat
    22441 gcatcaaaga atgcacctgc aagtacatgg tatccttccg ttgctcctgc ttgagccaag
    22501 gcagcatttt ctccacctaa tttcataagt atagtttctg taaaaggaag tgctatattt
    22561 gcaccatgaa gtccaacagt ccaaagtata tgaattataa atgcaataag cattgtagaa
    22621 cctaaactat cagctacatt tgatagtggt gctcctaaat aagtatttaa aatatctgtt
    22681 aagaaacttc cattagaaag catttttata aatattccta ttacagtaaa tatcattata
    22741 gttaacattc ctggtattaa ctttgcaaat gatcttgaaa ctgctggtgg aactccatct
    22801 ggcattttta tagttaattt ctttacctta gataatctta caaatatctc agttgataat
    22861 aatcctatta atatagaaga gaataaagct gctgtaccta agtaagtttg ttgtatcatt
    22921 ccccatcctt caactactgt agttcctccc tcaggaacta ttttaccaat ttgtggagcc
    22981 attataaaaa agatagataa agctataagc ccagattgta acccatcact ctcataagat
    23041 tttgctagaa aataagcaac acctattact aagaatactg ccattgtacc tattgctccc
    23101 caccaaaggt cgcctccaaa tgtggtccag ttttctccaa atatacttgc catcaagttt
    23161 ttgtatgctt ccaatggtaa gttatttatc aatgttccca atgcacctac catagttata
    23221 ggtatcatta ctataaatgc atctcttact gctactaagt gcctttgagc tcctatttta
    23281 gcagctacag gcactatata tttatccata aatgacatga atttttccat tttacatcct
    23341 ccagtacaac tttattttga actatttaac tctacagctc tatctaacac agcttttcca
    23401 ttacatcttc cataatccat catatttata acttctactg gaatattata tggttttgcc
    23461 atttcagttg cttttcctaa tacatatcta acttgaggcc ctaaaagtaa gacatcacaa
    23521 ttttctattt cattttttaa gttcacttct gatatagccc atatttttgc ttctataccg
    23581 ttttcctttg ctgcatcctc catttttgta actaataaac tagttgacat tcctgctgaa
    23641 caagctaaca ttatttttat cattttttcc cctccaaatt agattcatta aatatttttt
    23701 aagtcataag ttttttaaac tatacattta aaatatgctt ttctattaat tttttcttaa
    23761 tgtatatatc ttatttttaa atgtatatac atttaattct tgatattatt atataccaaa
    23821 tgtatataca tttcaagaaa actttttcat tttatttaaa ttttattcga caacagcatt
    23881 caaagttaat gtatatccat agtgatatgt gtgtgagtac tcaaaaatct cactattttg
    23941 taacttacaa atttggctta tatataaaag tggctctggt tcttctaatt ttagattatc
    24001 catacattct tcattcgata ttacagcttg tacttctctc tctgacatgg ctatcttgta
    24061 tccacacact tcttcaacaa aattataaag agaattttca caatgagtct tatttaaatt
    24121 tggaaataac ttaactggca tataagtaat catatatgaa actcttgcat cattagctaa
    24181 tcttactctt tttatttccc aaacattttc ttctaaactt atattcaact tacttgataa
    24241 attttcatct gcctgaacta tttcaagaga taccaatata ttagatactt tatagccttt
    24301 actttccatc tctttagtaa atccacttac agacgatata ttatcatata tattatttgc
    24361 aataactata ctacctacac ctctttgtct agttatatag ccttctttaa ctagattatt
    24421 aagtgcagac cttacagtca ttctagttac attaaattct tcactcagct gtttttcaga
    24481 agggattaaa tccccttctt tcaaactgtc tgaatttatt aactctctaa catgattttc
    24541 tataacttta taaattggct ctttcattta attcactcct tattaaatct tttatataat
    24601 aagataattt tatatacaaa caaagaaaat gtctatacat ttaagataat ttaaatttta
    24661 ttcagaaatc ttgtacaact ttccgagttt taataaagat ataaatagac ttatcattaa
    24721 tatacctttt aatatacttg ataaggttat actccaccat acaccattta gacccaacat
    24781 ttctggtcta gatataaagt aagccaatgg tactcttgca ccagtcagta tagtaattat
    24841 aatagaaggt atataggttc tccctaatcc tttgaaagct cctgttgtta ttatttctaa
    24901 acacataaaa aattgagaat agcctaatat tttaagataa actgctcctt tttctattgc
    24961 ctcactttca tctataaaaa tagaaaatat actctctcct gcaaacacta atactaaagt
    25021 agttattata ccaagtacag aagatacagt tatagctatt ttgcaacctt tatttattct
    25081 agaatacttc ttggcaccat aattttgtcc aacaaaactg cctaatgcaa ctgatagccc
    25141 ttcagccgtc atccaggaaa ttgcttctat ttgtgagcct actttttgta ctgcaacagc
    25201 cacaggcccc catgatgcta ctattactcc taatagcatt gagaatactg taaacatacc
    25261 actttgtatt gctataggta atcccaattt atacagaact ttataatatt tagtctctat
    25321 ttctttaaat agtcttaatt taaaatattc ttctttactt ttaactatta tatatataaa
    25381 acatgtagta actactatct gagcaaatac agttgcaata gcagctcctg caacaccaag
    25441 ttttggagca aatccccatc caaaaattaa tatagggtca agtataatat tagttattaa
    25501 tcctactgta ttaatacgaa aaggagtttt actatttcca agaccattga aaattgcagt
    25561 aaatactgga ttaataaaat aaaataccat acctagagct actattatca aatactgtct
    25621 agacatggtt ataacttcta agtctcctaa tctaaaaaaa cctatcaatt gtttattaag
    25681 tactattaaa gaaattgtat atattattgc tagtataata tttatttcta ttgctgactt
    25741 catatatgat ttagtagtgc ttatattatt tgcaccaata ctttgtgcta cttttatctc
    25801 tccacctact tttgaaatca taataaatgc cattgcaagc catggaaaaa aaccagctgt
    25861 tccaactgca gctactgctt tacttccggc tttaccaacc catatcatat ctatcatgtt
    25921 ataccccatc tgtataaatg aagtccccat tattggaagt gatagcttaa gtaatttttc
    25981 tgtaattctt ccttctgtta aattaagtct gtctttcatt ttccacctct tttatattaa
    26041 atttatttgc ttatatagct agattaatca ttttatttga ttgagttcac tatccttcaa
    26101 tttatattat catatctcag gattgcatct caatcatact tatgtttaag atacagtatt
    26161 ttacctatct atgataaaat taatacttat gaatataaat ctagccattt catcaataca
    26221 tattctaacc tttaacttaa cttcaattaa gtatacatat cgtaaccaat tagaatattt
    26281 tatacggtat cttccattaa taaacaacca atatcaatga tttatatata attttattta
    26341 tacataaaat agtatactat aataataaaa aaactatatt tgaatgcttt aagcatgttt
    26401 actaattgta atttccacac aaatttgata atatatgaat atattatggt gaaatttata
    26461 gatatatatt aattatgggg agattgtggg aggttctttt cacaagacat ttaagctatg
    26521 aaaataaata aaaaattcaa tagagttgat atgcaggttt caatacttac tgcaattatt
    26581 gtaataatat cttcctcatg tgtatatttt tttaattata cactaactta taagagcatg
    26641 atatactctt taagtgagag gtcaaaacat atctatgaat atgtagaaag aaatattgat
    26701 aaaaatacct ttactgaaat taattctaaa gaagaccaat caaaaaagtc ttataaaagt
    26761 tctaaaatcc ttttagaatc tgtaaaaaat actactggag tcatgtatct atatactgct
    26821 aaaaaaacaa aagatggatc actagtatat gttgttgatg gtttaaattc atctaggagt
    26881 gattttagaa atgctggtga tttgatagaa aaagagattt ggggagacct taacaaagca
    26941 ttagataatc atatattgtt acctaaaaag attaaatcaa catcttgggg atacatattt
    27001 atatcgtatt ttcctattca caatgatgtt ggtaaggttg ttggagtaat aggaattgaa
    27061 tttgaagcaa aacatcaata caatacattt aaatttataa gtatagttac accaataata
    27121 gcattattaa cttgtctaat atcagcattg attgcagtga ttctatttaa aagaatatct
    27181 aatcctacat atactgattt tgctaataca gattatttaa caggcctaaa aaatagaaat
    27241 gcttttgaaa tagatatgaa taacttaaac aattcaaatc taaagaatct aatatctatc
    27301 atatcaatag atttagacgg tttaaaaaag attaatgata aatttggcca tcagacagga
    27361 gacttatata ttaagcaagc aagtaaaatt atacaatatg taataaataa aaaatatata
    27421 gtttatcgta ttggaggtga tgaatacata attattctaa aaaatttatc tcacaaagaa
    27481 ataaataata ttataaataa tattaattta aaaatagttg aagaaaactc aactaatgaa
    27541 ttaaaattat caatgtctat tggatatgct atttttgatg aaaaagtaga tgtttcttta
    27601 tttgatacat atcgtcgtgc agattctcaa atgtatgata acaaaaggaa aattaataaa
    27661 caatatcaat aataaattta aaaagtctat tctttaaata ataaatcaca agaatagact
    27721 tttttaaatt tgtgttttta taagataaat tatttaatta aagtttgttc ttttttctaa
    27781 acgattcttt ataaattcat atattacttt cactaatata tttgtgacta atattaacac
    27841 agctagtgat gctgcctgtg cagtactccc cttatcatct agttgtacaa ttgatattgc
    27901 agctactttt gaacttgcag tatatataaa aactaatgct gatattgtca ccatggagtt
    27961 taggaaaaag tacataaagt tctctaaaac tgctggcaaa caaagtggta tagttacgtt
    28021 cttaagaatt gatgcgctgt ttattcccat tgatttagct accatatcca attctttatc
    28081 tactttctta atagcagtag tagctgttat aaatgctact gaaaagaaat gaactatatt
    28141 acatataacc attataagta atgttccata taatccatta aacaaattct taatgtatat
    28201 tgtatccata aaattaaact ctaatttatt aaaaaataat acataagcta tacctaaaac
    28261 taatcctggc aatgccatag gcaatgtact taaaaaatac ccaaatgatt ttaaatatgg
    28321 agctttttct gttttttcat tcaaatatgc aaataagaaa acaaagctcg tccctagaat
    28381 agctgtcaat agtgacattt ttattgaatt tataaatggt ttaatcccat caatactgtt
    28441 tttcatagaa aaatgttcca gtgtcagtct taaatcatat ggccaaactg atattaatga
    28501 agataagatt acagttaaca ttacagctat tataccaaat gcaataattc ctacaacccc
    28561 tcctaaaata ctatctctta acttattagt tttaacctta tatggaattg actttgaagt
    28621 tatatatgaa ttttgatttt tttcaatttt tttagttact ataaacgata ttattgcagg
    28681 tattaaaagt aatattgata cagttgctcc cattggcata ttctgttgcc ctataatttg
    28741 cttatataca tctattgata atacattaaa ctctcctcct atcacctttg gagctccaaa
    28801 atctgtaaaa cataatgtaa acgatacgaa tgcagaactt attaaagagt attttatact
    28861 tggtattgtt atgcttataa attctcttat cttgcttatc cccatagtat tactagcttc
    28921 atatagtcta taatcggttg aacttagaga tatataaagt atttgaaatg attgaggaaa
    28981 tacatatata atttgtgcca atattattcc cactgctcca tatattggaa catgcactcc
    29041 aagtaaatta gttaccaaac cttgatttcc aaataaatat actagaccta ttgcatgcat
    29101 cattgttgga gcaaacatag gaaacatagc taatgattta aaaaaacctt taaatttaat
    29161 ctttgttcta gttaatgcat atgcatatac taatgcaagt gttagtgata ttacagttga
    29221 tataactgat acaaatactg tattttttag agaatttaat agagattcag aagccaagta
    29281 ttcttgaaag ttttgtaatc ctacaaagac tccatcttta tctaaaaaag cctgtacaaa
    29341 tagatatatt ataggtgcta ataaaaatat tactaaagac ataactaata ctgtaagcat
    29401 acattttttt atatctaaat tcatttttga tttaagactt atacttttag aacttatagc
    29461 attattattc atacacttat gctcctttaa cttttggata ctttaaatag tatttttcgt
    29521 ctatacttat ttttacaagg tcaccttcta ctaaatttaa attaacccac tcttttatag
    29581 aagcatcact tattatgaaa ttttcattta atttatcttt tatttcaact ctcaataaat
    29641 tccctctaaa ctctatattt gatattattc cttggtagtt ttcttttgtc gatttctcta
    29701 tttgaacata ttctggtctt acagttaata tagaatctcc attatcaaaa cagtttgtat
    29761 ctcctataaa ttgagctgta aataggtttt gaggattttg gtatatttct tctggagtcc
    29821 ctatttgcat tatatctcct ccatttagaa tagctatttt atctgccata gttatagctt
    29881 cttcttggtc atgtgttacc attattgtcg ttatattaag ttctttttgt aatcttttaa
    29941 tgtccattct aagcttgtgt ctaacctttg catctaatgc ggacattggt tcatctaaaa
    30001 gtaaaaactt tggttcaaga gctaatgctc ttgctattgc aatcctctgt tgttgcccac
    30061 ctgataatgc tttaggatat ttatgagctt cattagtcaa tccaactgtt tcaagtactt
    30121 ccttaacttt attatctatt tttccttttg aaacttttct ctcttttagt ggaaaagcta
    30181 tattgttata agcagtcata ttcggaaata atgcatatga ttgaaatact ataccaaaac
    30241 ctctctttga tggctctaaa tttgtaatat ctttatcctg gagaattata gttccgctat
    30301 ttacatcttc aagacctgct attattctaa gtaatgtagt tttaccacat ccactaggtc
    30361 cgagtaaaca tagaaattct ccttcttcaa tatccaaact aatattattt aaaacccttt
    30421 tttggtcata acttttaaac acattattta tttttaagta actcatatct tatctctcct
    30481 tatatagtta tttttttatt ttaaatttta tatcctactt tttcttctaa aattctttca
    30541 tctaaattat taatttaaac tttacttact ttcagatttt gaaccgtatt ttgattccca
    30601 agttttaagt actgtacttc tgtttttggc agcagagttt aaatcaactt cttttataag
    30661 ctgttcaaat ggttgtttag aataaccttt tggaactgga ttatctgtct ttatggaagt
    30721 aactgctact tcatgtgcat atctatccat agctgaatca cttattgccc aatctaaaaa
    30781 tagctttgct tcttttttta tattatcttt tttaactaag gcatttgctt ctaagtccca
    30841 gcctgaacct tcctttggaa atacaacctc tataggttct ccactagata attgttgtgt
    30901 agctctatat ccaagtgtaa tacctattgg atactctcca gagccagcta acttcgcagg
    30961 ttttgaaccc gagtgtgtat atgtggctat atttttatgt aatttatcca tgtaattgta
    31021 agcatctttt tcacccatca tttgaataag ccctgatact gtaagatacc ccgttccaga
    31081 tgatgctgga tttggcattg ttataagtcc tttatattct ggttttatta aatcttcata
    31141 actttttgga atatctatac ctagtttctt aagttcctta taattaacta ctattgctgt
    31201 ctctgggaca ctcattccta cccaagatac atcatcttca gtatctttaa attttttatc
    31261 aactttatca taatttttag gcttatatgc ttcaagtgca ccatcttttt ttagctctat
    31321 catgttcatt gctgataatc cccatataat atctgcttgt ggattatctt tttctgctaa
    31381 tagttttgaa gctattacac catgagaatc cataactatc tctacatcta tattgggata
    31441 ctcttctcta aaaccttcca tataataagc tatctgctct tcctctagtg ctgcatatat
    31501 tgtaagttta ttatttcctt ttccttgagt agtttgtggt gttgttgata tgcaacttgt
    31561 cataaatgta atcactacaa tgcacagaca agtaaatata aattctttca tatcttcccc
    31621 ctttgtgttt tttccttttt ttatttgttt tttattgttt ttgcttatgt ttatataata
    31681 cacctaattt gttaatataa agttaagtgt aaattaacag ttcgttaatt tttagaaatt
    31741 tttgttaaat atattatttt tagttaaata cagtctgttt ttaaacaata tattaatttt
    31801 tatagtatct acattttaat ttaaacataa aaaataagtc taacctctat aaagtagaat
    31861 gttagactta tttttataag tatttaatta tacaaccaaa tcattttttt tcttttcttc
    31921 aagtgctttt ttaagcacca tatccttaac cttcataagt ctagtagtac ttgctctctc
    31981 attttcttca agcttcatag ctatatactt aatagtctca atgtaatttg gtatcattac
    32041 attttcaaga gcattaacac gtctacgtgt cttttctatc tcttgtgcaa gaagctgtgc
    32101 acttttttca ctttctgcaa gtcttaaaag tggttgcata gcatctgaga aagcttccat
    32161 agcagaatct aattcacctg atgtaaaagc aagaccataa ggataaatat cactttgatt
    32221 gttttcagtt ttaaagtcaa acacaggaac atcaacactc attatatttc tcgtagaaac
    32281 atctactgaa actgattgct ttggcatcat aagtgctgaa cccagatact cctgactcat
    32341 aactgctctt gcgattataa agtttttata agcagtatca agagcatttt ctgcttcttc
    32401 acgcaatcgc ttattttctc ttacaatctc aagaaattgt ttcataagtt catcaagttt
    32461 atctttaaga agcttatgac ctcttgtagc agttttaagg agtttcttaa gtctagtcat
    32521 ttccatacgt gttggattta tatttaatct agccacagat atcactcctt aggcatatat
    32581 ttttcaaaat attcatcacg gatacgttta agttcacttt ttggtaacat actaagaagc
    32641 ttccatccga tttctaatgt ctgctcaata gttctatcag ttctaaatcc ttgtgaaaca
    32701 tattctttct caaattcatc tgcaaactta gcatacatta aatcaatctc agaaagtgca
    32761 gattcaccaa gtatagccat aagttctttt gcatccttac ctcttgaata agctgcaaaa
    32821 agctggttca ttgtatctgc atggtcttca cgagtcttac ctttaccaat acctttatct
    32881 tttagacgag aaagtgatgg taaaacatct ataggaggct gtatatcttt tttataaagt
    32941 tcacggctaa gtataatttg cccttctgta atgtatcctg taaggtcagg aattggatgt
    33001 gtcttatcat cctcaggcat tgtaagtatt ggaatcatag ttatagaccc ttcatgccct
    33061 ttcatttttc ctgctctttc atacatagta gcaaggtcag tataaagata tcctggatat
    33121 ccacgacgtc ctggaacttc ttttttagct gctgaaactt cacgcaacgc ctctgcatag
    33181 tttgtaatat ccgttattat aacaagtaca tgcatatctt gctcgaaagc aaggtattct
    33241 gctgctgtaa gtgccattct aggtgtagat acacgctcta cagctgggtc atttgcaagg
    33301 tttacaaaca ctacagaacg gtcgattgca ccagttgctt taaaatcgct tataaaaaat
    33361 tcagcatctt caaaagttat accaactgca gcaaatacaa cggcgaactt agaatcagtt
    33421 ccacgtactt ttgcctgtct tgcaatctga actgcaagat tggcatgtgg caaaccagac
    33481 cctgagaata taggaagttt ttgccctcta accaatgtat taagtccatc tatagcagag
    33541 actccagttt gtataaactc ggccggataa tcacgtgctg ctggatttat tggagctcca
    33601 tttatatcaa gtcttttatc tggtataatt tctggacctc catcaatagg tctacccatt
    33661 ccactaaaaa cacgacctaa tatatctggt gaaactccaa agtcaagact ctttcctaaa
    33721 aaacgaactt tactttcagc aaggtttata cctgttgagc tttcgaaaag ctgaacaagt
    33781 gcagtatctc catttacctc tagaactttg caacggcgaa tttctccatt tgaaagttca
    33841 atttctccaa gttcatcaaa ttttacacct tctacgccgt ttattaacat aagaggaccg
    33901 gctacctctt gtattgactt atattccttt atcatagttc ttcagccccc ctctttttaa
    33961 taaggtctgc aagctcgttg tctatttcat cttcaattga atcgtaagct gcatctatat
    34021 cgccttcttc tacatactta gcacgaccaa tcttttcaag aactgaaagc ttgataatat
    34081 cgtttatagt aacattttgc ttgatagcat cttgtgctga cttatagaat tttaatataa
    34141 gtcccatcat tctatattgt ttatgaagtg atgtataagt atcaacttca tggaaagagt
    34201 tttgatgaag gaagtcttct cttatagaac gagtaacctc tagtatcaat ctatctccat
    34261 ctgaaagtga atcaacccca accaattgaa ctatttcttg aagttcagat tcaacttgta
    34321 aaagtttcat agcttgagct ctttgagctg agaaattatc atttacattt ttatctgccc
    34381 atacatcaac tttttcctga tagagtgaat aactagtcaa ccagttaatt gctgggaaat
    34441 gacgtttata tgctaaagat gcatcaagac cccagaatac ttttactata cgaagagttg
    34501 cttggctgac aggttctgat gtatcaccac caggaggaga aactgcacca attgctgtaa
    34561 gtgttccttc tctttcatcc atacctagac aatttacctt tcctgctctc tcatagaatt
    34621 gagcaagacg tgaacctaag tatgcaggat aaccttcttc accaggcatc tcctctaaac
    34681 gaccactcat ctctctaaga gcctcagccc atcttgatgt agagtctgcc ataagtgcaa
    34741 cactatatcc catatctcta aaatattcag ctattgtaat accagtgtat atagaagctt
    34801 cacgtgcagc aaccggcata tctgatgtat ttgctataag cacagttctt tgcataagtg
    34861 actcgcctgt tcttgggtct tttaattcag gaaattcaag aagaacatct gtcatttcat
    34921 ttccacgctc gccacagcct atatatacaa ctatatctgc atctgcccat ttagcaagct
    34981 gatgctgtgt aacagttttt ccacttccaa aaggtcccgg tatagctgct accccacctt
    35041 ttgtgattgg gaaaaatgta tctataactc tttgacctgt aacaagaagt gaatctggtg
    35101 ttttcttttc tctatatggt ctttcttttc taactggcca tttttgcatc atagttacag
    35161 gtatatcatt acctttttca tctgtaataa cacaaactgt atcttcaaca gtaaattcac
    35221 cagaatatat ttcttttata gtacccttaa ctccataagg aaccataatt ttacattcta
    35281 ctactgctgt ttcttgaacg ccaccaatta tatctcctgc tacaacctta tcaccaacag
    35341 attttttagg tttaaattcc cacttagttg ttctatcaag tgaagatact tcaacacctt
    35401 tagttatatt agtacctgaa atatcataca ttttttcaag tggacgttga ataccatcat
    35461 aaatagttga aataagacct ggtccaagtt caacactcat aggcattcca agagaaacta
    35521 cttcttcccc tggtcctaga ccggaagttt cctcataaac ctgtatagaa gctttatctc
    35581 catgcatttc tataatctcg cctataagat gtttatctga aactcttacg acgtcaaaca
    35641 tattcgcatc tctcatgccc tccgctacaa caagcggtcc tgacaccttt acaattgtgc
    35701 ctgttttcat attctctcac ctgcaatcat atactttatc aaaatttaaa tattccctcc
    35761 tatattttat tagcacatat atttagcacc aataaaattt gagattgtct tttattcaac
    35821 tttcatctat tctttaaata atatatctgc acctatagca cgtttactag attctcttac
    35881 ttcattcata cctaatccca tacttcctcc aatacctgga ataactataa tggcaggaag
    35941 ttggtaatct ttgtattttg ctattgattc ttttgcaagc aatgatgcct gttctgtaat
    36001 ataaattatc gcatattcat cttcaaccaa cttatgtata ctttttttaa tttcgtcact
    36061 atcataggct gggaagatat ctattccaag agctaaaaat cccatgatgc tatctttatc
    36121 tccaactact cctaccttat acataagcat cccttagcct ttcttttata gtatctgcgt
    36181 ctatattatt aagcttacta gtcaaaataa ttctaattgt ttttatctct gattctttag
    36241 catataaata tgcaattagt ggttctaatg taagaggctt aaattttgct cctctaacat
    36301 attccataag atagtcatca caaagttttt caaagacagt aaaaccacta tccatgccat
    36361 ttttacagac atcagagtaa gaagtagatt taaaacatga tgctggagta tctgaggaaa
    36421 aagcttctaa gaatttttcc ttatcaagtg agccacctga aacaaacaca ttcataaaca
    36481 tatcaaaatc tttcttcata tttttgactc ttataaaact ctttagattt gttaaatcac
    36541 aaactttcat aacataatct attacaaatt tgttttggct ttctttagca gtttctttca
    36601 tactggtaaa ggttgcttta tctagaacaa tatcaaccat ctggccattt tgtgtcttat
    36661 tataaacatc aaatgcctct aatatagctg atgacattat cttaggtaaa tcactaaaac
    36721 tacgatttgc taaagattct ctaagcttaa taagaggaat tgtacctcca tcaattaaat
    36781 atttctcacc atctacacca gaaatttctt ctttaatcag cacttttaaa ttgtgatagt
    36841 catttttata aagaaataca tcaacaaatt tttcttcagg agctagactt ttaagaattt
    36901 catatgtctt tgataattct tgagtcaaaa tattctcaaa attatgtata ttattatttg
    36961 atgtatcagc ataacctgcc tcagctataa ttcttaaaga gtcttcaggg cttttggctt
    37021 ctgccatttg aataaacatt tgtctgttaa gaagctgttt ttcaagcact cttattcgtg
    37081 caacggcata gggataagtt ttttcttctg ccatttttta acccctcctt aattaaacaa
    37141 aatttctgct gcaatttgtt caatatattc atgctctact gcaattatgg cttcaaatga
    37201 ataattgtac tcaatgtcac cttttttaac tatgaaacct ccagtaatat ctctagtttc
    37261 atcagacact ttaatgcctt tttttgatag cacatctttt aatgtttttt tatctttttt
    37321 agataatact atctctgaat catctgtaca atttgatttt tcaatcatgt ttagtataat
    37381 ctcttcatat tcattatctt taagatttaa aagtttgttt ttagcttcta aaattacctc
    37441 ttctaaaatc tcttgttttt ttgataatat ttgcttttta gcctccatat aagctcctga
    37501 tatttcctta gaagccgctt tttctgcttc tgcctctgca agctttgtat aggaagccat
    37561 ttccttttca gctttttcat tagctgaatt tacttttaaa tctgcttcac ttttagcttt
    37621 atccaatatt tcttgtgctt cttgtttggc atcagctata attctgtcaa tcattttttg
    37681 ttcattaccc ataggagtgc ttcctccttt tcattttttt tacttatgaa ttataattta
    37741 taattaacca attgcaattc cattgtaagc taagaaagaa acaagtagtc ctaaaagagc
    37801 atatgtttca actattgctg caaatgttat agctttacca agttcttcag gtctttttgc
    37861 aactattcca actcctgctg ctgctgcttt accttgtgct atacctgata ctaaaccaac
    37921 taaaccaatt ggaagtcctg ccataagaag ttgtaaacct tgagctgatg ttggtattgc
    37981 atctccacca ataattcctg cttttgataa aataaggaaa gctatgataa atccatacag
    38041 accttgtgta ccaggaagaa gttgtaaaac tagaagttga ccgaatttac tagggtcttc
    38101 tgttacaaca cctgctgcgg cttgacctgc tattgaagta cctttagcag aacccatacc
    38161 tgcaaataat gctgctatag cagctcctga tattgccaaa aattgaccat ttcctaacgc
    38221 gtttaaaaat tccatattta catctccttt ttattaatgc ttgtatattt tgttttatac
    38281 ttaaacggaa caaaaggaac tcctccgcct tcataaaact tattgaaaaa ttctacatac
    38341 tgtaacctac ttgtatgaac atatgcacct agtgcattta taagaaggtt tattgtgtgt
    38401 cctacaacac caattacaac tgcaagtatc ggaccagcta tagctcctaa aaggttgata
    38461 acttgtgcta taactcctgt tgttaaacaa agcgccataa ttcttgtata agataatata
    38521 tcagcaaaat aacttgttat accatagaga cttgaaaatc ctttaaaaag cttaacagga
    38581 atacctttaa agcttcttcc ttgagtaagt acaagaccta tagcacctat aattgctaaa
    38641 tattttccta cttctgaaaa tacacctata tcccctgcaa caaaaggtat tataagtaga
    38701 catacacctg ttatacacaa ataccaaaaa cctatgtcaa agattgcatc tattaccttt
    38761 ccacttctta ttaatgtata tgcttttatt cccaaaccta catatatatg gataataccg
    38821 aacagtaacg acattcccat aagtgtcata acatcctcta aaggattaat taatgctttt
    38881 ataggaatta aatcccccaa aatacccccg aatataagac cccatacagt agttgaaact
    38941 ccacagatac caataagttt aattaaattt ccttcacctt tttgtatctt taatttatat
    39001 acaacaaatc cacagactac tgcaattatt aacccatatg cggcgtcact tagcatcatt
    39061 ccaaagaata caacaaagaa gaaagttaaa attgtatttg ggtcaatatc ttttgtactt
    39121 ggatagctgt acatgttagt tacactttca aaaggtgtta ctaatttatt gttttcaagc
    39181 agaattggga agccttcttc tttacttcct tcttcagtct gaacacaaca attaaacttt
    39241 tctgtaattt ctttaattaa ttcatctgcc cttttgcttg gtaaccaacc attaagacag
    39301 aatgttgttt ttgttttgac aagtctctct ataatctttt tttggtctct ttctatagtg
    39361 taaaaatcat ataggttttc aagcatgtgt atactatctg catgtctttt aatttcctct
    39421 gttttgtcct tagaaacgtt ttcctgtcta attatcattt cattgtattt cttgatagct
    39481 tgtaacggtg taccttcttc atttggtaat gaaaccactg aaaaattaaa ttcttttaaa
    39541 acttctaaaa catcatcaaa agtttctttt aaagtaatta aataaacata gttcattaac
    39601 ttgtcagaat tgacaatccc aataacactt tctggtattt catcttctaa ttttactctt
    39661 aaagcatcaa tgtttgcgct tatgggcaca gttccaagta tagtttttgt atacttagtt
    39721 tccatatttt caagaggaat atcaaagttc acccatggtt ctaacatact tttattgttg
    39781 ttaagtaagt ttatgttaga ccttattgta cctatctcct tgtgcaaatt gtttaactcc
    39841 agagcttcag cataaacttg cccttctttt tcactatcaa ttttgttaaa ctctcttttt
    39901 ggtgcaagta gggatttttt ttgtttcact agtgaagaaa caatattaat tgcttggttt
    39961 acatgagaca ttttctgctc tagcattata acttcagatt cttcactatc ttttacaaga
    40021 taatttttta attcctcctc ctcagtaaga aaggcactat catcaatctg aacaaaccca
    40081 cggttcataa gtagctttaa aatctggctt ttttgagctt ctaagccaac aatagatatt
    40141 ttgttcatat taactattgc catattatct cactaccttt ccaataaccg cttcaatagc
    40201 cttacttttt ctttcttcag cagttttttc aattttcaga cattcacttt tagcattagc
    40261 taagatttta tcaacctctg ctctactgtc ggtgactgcc ttctcaataa gagtttcttc
    40321 cataagcttt gcttgttttt ttacatcagc aaatatgctt tcacatttgt ttacagcatc
    40381 agctctgata gactctgcat cagtaacagc ttttactttg atactgtctg ctgtctgctc
    40441 tgcctcaaca atgcttttta ttatttcaaa agccatccaa atcacctctc ttaccttttt
    40501 actatgtata taaccaatta gat
//
LOCUS       DAGSHT010000015        37752 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGSHT010000015 DAGSHT010000000
VERSION     DAGSHT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.016"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(128..907)
                     /locus_tag="MJB72_000756"
     CDS             complement(128..907)
                     /locus_tag="MJB72_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBY8261881.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(900..2327)
                     /locus_tag="MJB72_000757"
     CDS             complement(900..2327)
                     /locus_tag="MJB72_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY8261882.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            2718..3440
                     /locus_tag="MJB72_000758"
     CDS             2718..3440
                     /locus_tag="MJB72_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8261883.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            3637..5304
                     /locus_tag="MJB72_000759"
     CDS             3637..5304
                     /locus_tag="MJB72_000759"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBY8261884.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMNDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(5400..6719)
                     /locus_tag="MJB72_000760"
     CDS             complement(5400..6719)
                     /locus_tag="MJB72_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY8261885.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKVVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(6760..7506)
                     /locus_tag="MJB72_000761"
     CDS             complement(6760..7506)
                     /locus_tag="MJB72_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBY8261886.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(7750..9759)
                     /locus_tag="MJB72_000762"
     CDS             complement(7750..9759)
                     /locus_tag="MJB72_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261887.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEAMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(10197..11630)
                     /locus_tag="MJB72_000763"
     CDS             complement(10197..11630)
                     /locus_tag="MJB72_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY8261888.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(11646..13070)
                     /gene="ascB"
                     /locus_tag="MJB72_000764"
     CDS             complement(11646..13070)
                     /gene="ascB"
                     /locus_tag="MJB72_000764"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBY8261889.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(13157..15073)
                     /locus_tag="MJB72_000765"
     CDS             complement(13157..15073)
                     /locus_tag="MJB72_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8261890.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(15147..16007)
                     /locus_tag="MJB72_000766"
     CDS             complement(15147..16007)
                     /locus_tag="MJB72_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8261891.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(16358..17545)
                     /locus_tag="MJB72_000767"
     CDS             complement(16358..17545)
                     /locus_tag="MJB72_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8261892.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(17559..18950)
                     /locus_tag="MJB72_000768"
     CDS             complement(17559..18950)
                     /locus_tag="MJB72_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBY8261893.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(19545..21245)
                     /locus_tag="MJB72_000769"
     CDS             complement(19545..21245)
                     /locus_tag="MJB72_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY8261894.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(21217..22620)
                     /locus_tag="MJB72_000770"
     CDS             complement(21217..22620)
                     /locus_tag="MJB72_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8261895.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(22884..23375)
                     /locus_tag="MJB72_000771"
     CDS             complement(22884..23375)
                     /locus_tag="MJB72_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8261896.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(23401..24213)
                     /locus_tag="MJB72_000772"
     CDS             complement(23401..24213)
                     /locus_tag="MJB72_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY8261897.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(24226..25377)
                     /locus_tag="MJB72_000773"
     CDS             complement(24226..25377)
                     /locus_tag="MJB72_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY8261898.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARILNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            25647..26288
                     /locus_tag="MJB72_000774"
     CDS             25647..26288
                     /locus_tag="MJB72_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBY8261899.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            26314..27858
                     /locus_tag="MJB72_000775"
     CDS             26314..27858
                     /locus_tag="MJB72_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8261900.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(28058..28684)
                     /locus_tag="MJB72_000776"
     CDS             complement(28058..28684)
                     /locus_tag="MJB72_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY8261901.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(28819..29535)
                     /locus_tag="MJB72_000777"
     CDS             complement(28819..29535)
                     /locus_tag="MJB72_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBY8261902.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            29961..31175
                     /locus_tag="MJB72_000778"
     CDS             29961..31175
                     /locus_tag="MJB72_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBY8261903.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDQYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIISSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            31793..33121
                     /locus_tag="MJB72_000779"
     CDS             31793..33121
                     /locus_tag="MJB72_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261904.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(33259..34449)
                     /locus_tag="MJB72_000780"
     CDS             complement(33259..34449)
                     /locus_tag="MJB72_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8261905.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(34555..35934)
                     /locus_tag="MJB72_000781"
     CDS             complement(34555..35934)
                     /locus_tag="MJB72_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8261906.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(35965..37173)
                     /locus_tag="MJB72_000782"
     CDS             complement(35965..37173)
                     /locus_tag="MJB72_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261907.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT        12584 a         6656 c         4302 g        14210 t
ORIGIN      
        1 tgtacttcct atattgatag agatgattca agtttttatt ttgatacatc tctatcaata
       61 tttttttaga aaatacacat ttttttcaaa tacttatttt taattaaaac tttaggtaat
      121 ctattcttta tatataatta ttagacttga aaatatcttt aaaatcaacc aattcaatat
      181 tatttctttt taaccacatt ttaacttcct tatcacatag cattgcatgt tcatatactc
      241 tctcttgaat aagagaagat gattttataa gactataatc tataaatcct ggatgagtaa
      301 ccatcaatgt tatatcttta tctaaattta tatatttttc ttcaaataat ttaacaaaag
      361 atttatttaa cttgtaaaaa tcattctgag gatatttact tggcatgcta atacatgaat
      421 ccaaataata gggacaactt ggtataccat attcttttgc agcatcacac attgctgata
      481 ttgatacatc ttcttctata ccatgtatat caatatactc aggcaattta ctcatataca
      541 acttgtaagt ttcaatttga gcacaaactt cttcatatac ttcttcataa ttaaaaacct
      601 ctttacctgt cagtagttgc gttcttctta ttttgctaga aacaaatttt ccaccttcat
      661 ctaacaaatt ctttatctta atatgttctt tacaacaagg ttctcctatt actaaattaa
      721 catgtaaacc aaaacataca tcatcatttt ttaccatttc tatagcttgt cttgaaaaag
      781 gcatattcac catgattcca gcacttgtaa caataccgtt cttataagaa gatattatcc
      841 ctagtgtaac accttctgaa tatcctatat catctgccct aactactaat ttcttcattt
      901 tatgcatctg cctgctttct caactcatca agttgcattt ccatctcatc ctttacttta
      961 acaacatccg ttcctatgat gatttgaact gatgtattac tcaatttaat tacccctttg
     1021 gctcctgttt ttttaatatt atcatcattc aaaatagtag catccttcac ttctaaacgt
     1081 aatctcgtca tacaattatt aagagtcact atattttcat aaccaccaca atttttaatt
     1141 atctgtttag ccatatatga aaaattacca tgagaaagtt ttaaattctt ttctttttca
     1201 ctaactttat cacctatctc tatctcaact tcacgtcctg gtgtttgcac attatctttt
     1261 attattatat atttaaatgt tacataatat aatacaaaga atacaactcc tacaggtata
     1321 actaaccacg ctttatctcc ataactaaaa cttaagacat agtcaattat acttgctcca
     1381 ttaaatgaac ccaagcgagc acccagtaaa tatagtacaa ctccacctag accagaataa
     1441 aaagaatgaa ttatatataa ctttggagat gcaaataaaa acgaaaattc tattggttca
     1501 gttactgttg ccataaaaga agtcaatgct ccagcggtca ttgcaccttt tacttcttgc
     1561 ttctttttat cttgtgctgt tttatatata gcataagaaa tagcaggtat tgaaaacatc
     1621 ataattatgt aaaatcctgt ccagaagaat cctgcatttg ggtctcctgc cataaatcta
     1681 ggaatttctc ctcttataac ttctcctgtt gatgttgtat attcacccat ttcaaataat
     1741 atgtaagcat ttaaaacttg atgtaaccca gttggtatta gtgcacggtt acagaataaa
     1801 aacaagaata ttcctattgc accagctcct cccaaccaaa aaccaaattg attcattgca
     1861 gcttgtggat atatccataa taaactaaat atatatgata aaattaaagc aaccactggt
     1921 gatagtgtaa ttacacattt ttctcctgca aagaagctta aaaatgctgg aactttaact
     1981 ttatatgttt tattgtaagt ccatgctata caagctccaa caattatccc accaaataca
     2041 cctgcattta atgtatcatt caataactct aaatttgact taaatatcat atatgcaact
     2101 acggctgcaa ccattacact tgtcttgtct ttagactttg agtaggcact agctgctcct
     2161 aataaaaata agtagtccac atttgaaaat actatattag atatattaga tatagctggc
     2221 acattgagca tatttggacc aaataataat cctaatatac ctgctgctgg cattacagct
     2281 attggcacta gcatagtctt tcctaaaagt gacagttgtt gcatcatttt gtttaagaac
     2341 ctcttcatat ccaatcccct ttctatttcg gtttaatttt ttgtgaaaac gatttattca
     2401 cattttatat tttaaatatt atatttgttt aaacaaattg tcaatatttt ttattcattt
     2461 tatttttatc atttagttta acatataaat ccttttaagt gtattgctat atctagtcta
     2521 tttttttaat tcattttttt tattttaaaa atatgtttaa ccatgtattt gaaacaaccc
     2581 acaaaataat tctattttgt cctttttatg gttgatatga ttctattttc actatacatg
     2641 tctaaacaca tttgatataa tttgttcata tcagttcaat aatattacta aagaccttta
     2701 aggagggata ggggtatatg gctaaaaata aattttcaga gatatatgaa gtattgaaag
     2761 aggaaatact tgatggtaaa tatacaagta atatgatgct gcctactgaa cttcaactta
     2821 ttgaaaggtt ttcatgtagc agaaacactg tacgtagagc aatttctcaa ttaaacacag
     2881 aaggatatgt tcaaagtata aaaggcaagg gtgttgtagt gttagaaaat agttgttcta
     2941 atgatttttt tctaaatatg cacaatttca aaggtgtaga atcgattgta gaagataaaa
     3001 aggttaatac tgctactagt gttctccact tcagcaaaat cttaattgat aataagttat
     3061 ctaaaaagac aggttttaaa gttggtagtg aagtatatta ccttcacaga ctaagatata
     3121 ttgataacat acctaaaatt ctcgacatta actattttct atgtagtata gttaaagacc
     3181 ttgatgtttc tatagcacaa ggctctattt ataaatatat agaagagtgt ctaggtacaa
     3241 aaattgtttc atccagaaag atatttaaaa ttgaaaaagc tacagagctt gagttaaaaa
     3301 cgctacctct aaatgattat aattgtgttg gtgttattaa aaatagtgta tatactgatg
     3361 atggaaagct ttttgaatat actgaatcaa agcatacacc tgaaacattt gtattcatgg
     3421 atgttactca aagatattaa aaaaattatg tttaattaaa cataattttt ttacgtttag
     3481 acactttatc ccatactaac ctcactattc tatatacaat tatatgttta tacaaataat
     3541 tatttattgt atattttagc tttaatatta gtttaacgct tgacaatttg tttaaacata
     3601 ttataattaa cttataaatt acaggaggta tcaagtatga aaaattggtg gaaaaaagca
     3661 actgtctatc aaatatatcc aaaaagtttc aaagatagca acaatgatgg tataggtgat
     3721 ataaatggta ttatagaaaa attagattat ctgtattctt taggtgttga tttattatgg
     3781 ttgactccta tgtacgtttc tcctcaaaga gataatggat atgatataga agattattat
     3841 aatatagacc caaaatatgg gacaatgaat gactttgaaa aactcttaaa agaagctcat
     3901 aaaagagaca taaaaataat gatggatatg gtcctaaacc atacatctac tgaacataaa
     3961 tggtttaaag aatctaaaaa aagcaaagac aatccatatc gtgattatta tttctggaaa
     4021 gatgctaaac ctgatggttc tgttcctaat aactggatat ccagatttag tggaactgct
     4081 tggaaatatg atgaaaccac taaccaatat tatttacatt tatttgaaga aactcaagct
     4141 gatttaaatt gggaaaatga aaaggttcgt gaagaatgtt acaaaatact agaattttgg
     4201 gctgataaag gtattgatgg ttttcgttta gatgtagtca acctactctc caaaacacct
     4261 ggattacctg atgatcctat aacaggacca aaaggagatg gaagaactca ttacgctgat
     4321 ggtcctcgta tacacgaata tttacataat atgaatcaaa aagttttcaa acctaaaaat
     4381 atagttactg taggtgaaat gtcatctact acacctgaag agtgtatcaa ctatactaga
     4441 gaaaatcgag aagaactatc tatggttttt agcttccatc atatgaaaac agattataaa
     4501 ggaggcgcaa aatggactaa tgaaatgttc tctctggata aattaaaaaa agcacaatct
     4561 gactttcaat ataaaatgta tgaaggaaaa ggatggaatg ctctatttta ctctaatcac
     4621 gaccaaccta gagctttatc tagatttgga gatgatagat tttttcgtca agaaagtgct
     4681 aagatgttag ccataacatt atttggtcta caaggtacta catacatata tcaaggtgaa
     4741 gaaataggta tgacaaatgc ctactttact aaaatagatg aatatgatga tgctgaaagt
     4801 aagaatgcat actatcatat gattaaatct ggtattgatg aaaaggaggc attgcttata
     4861 cttcaacaaa aatccagaga taatgcccgt actcctatgc aatgggataa tactatttat
     4921 aaggggtttt ctaatcataa accgtggtta aaagttaata atgacaatat tagtgtagaa
     4981 aatgagctta atgattctcg ttcagtattt tatacttatc aaagattaat caaatatcgc
     5041 aaacaatatg atatttttac agaaggtact tatagattgt tagatgaaaa tcataaaaat
     5101 ctatttgttt atgaaagaga gtataaaaac caaaatcttc ttgttataag taattttact
     5161 catgataatg tagtatataa attacctgaa acttatttga ataaattaaa ctacacaata
     5221 cttgaaacca attacactag agatataatt gaaaataaaa tggaaataaa accttacgaa
     5281 agtataatga tatattataa ataaagttat ttatgtaaaa atcggtataa caccctggtg
     5341 ttataccaat tttactaaat tcaaagtaat atttatagaa tatctataga ttacaataat
     5401 tatttgactt ctacatcatt atatttcttt gaatatactt tagtcatata gaaatatata
     5461 ggaactacaa ttatcatccc acctaatccc caaactatct tcataggttc atcaatacct
     5521 gcttttatca gtaataatat acttataata actgcaaata ttggtataat tgctcctcca
     5581 ggtattctaa aatttacaac tttaccagca tacttctttc tcaatattag tacagctaaa
     5641 catgtaggaa tatattgaat aaatctagat acaacactta tttgagataa tgttgtgaat
     5701 gaacccgacc atgctattag taaagttcct atgacagata ctataattgc tatataagga
     5761 gctccatttt tattagtttt tcttatagac ttaggcatta atccttgttc aaccaaagca
     5821 gcagcagacc ttggtgtaaa tatggatgat gcaatattta tacctccaac agatactata
     5881 gttcctattg taattatata cccacctata ggacctaaca tcttaatggc agcatctgca
     5941 acaggagtag ctgaccctgc taagccatta cccaacaccc ctatacttat tcccagtata
     6001 agcatatata ttactgatac tacaaacatt accattacca atgctttagg tacatttttc
     6061 ttagggttct ccatatcttt tgctgcaaca gctaaagatt caaatcctgt aaaagcataa
     6121 aataaagtta tagatactgc tacaaaagct gatgacgcac tttgaccttt ttgtattatt
     6181 acaaatgggg taaaattaga accctttata aagaataatc ctacagctat aaataaaact
     6241 attggtaata attttcctat agtcatgata ttatttaagt gcttagattg tttcattccc
     6301 ataaaattta taatcccaag ccctacaact aatataccaa ttattatatt tttaataagt
     6361 ggttctgctg cttgtggcca tacagatgat agtgttacag caaagaaatt tgccatagta
     6421 gcccaagcaa tcatactaac tatccacttc ataaatccaa cttcaaatcc aacaaaatct
     6481 ccaaatgcct cttttgcata aagatagggc cctccatcag tatcaaacca actacctgct
     6541 tcagcaaaac ataaagcaat agaaacagct aaaagcatac aaaaaactaa tactaatata
     6601 cttgctggac ctatatctgt ataagcatca ccaggtaacc cgaatatacc tgaccctatt
     6661 attgcattga ttcctaatag tacaatactc caaaaaccta acttaatgcc tttttccatt
     6721 gttattcccc ccaaagccaa cttaatatat ttatattttt tatttagatg cttctttaac
     6781 tattgccata aatatttttt tcatattatc ataatctcta gtcatcattt ctggatgcca
     6841 ttgtatccct attacaaact cacttccttc cttttctata gcttcaatta aaccgtcttt
     6901 agatgtagct gaaacaatat atcccggtgc tactttgttg acaactaagt gatgaaaact
     6961 attaactaaa acttcttttt ctcctaatat ttcatataat tttgtcccct cctttatctg
     7021 tactgtatgt gtaggtacat tagatagatg ttgctgattg tgtttaatat aagcaccttc
     7081 aattaaagat aaatcttgat acaaactacc tccttcagtt acatttataa tttgttctcc
     7141 tctacatata cctaaaactg gttttttcat atctaaagca tgttttaata atttcatatc
     7201 aaaactatct ctctttggtg atattgcccc caacttatta tgaggttctt ctttccatat
     7261 gagtggattt acatcttgtc ccccagatag tataagcgca tctatattag atacctgctc
     7321 ttttataatc tcatcatcat atacaatcgg tacaatatat ggtatagctt tacacataac
     7381 tactgattgt atataatcat tatttacata agccctttca tagccaggaa acattcctcc
     7441 ttcatcaatt agtaaattac ctgatattcc tattattgga tatttctttt tagccaattt
     7501 cttcacccct taacttattt gtgaacaata aaaaatgcta cttctcttaa agagcaagta
     7561 gcataccaag attttattta tttgaacatt ataatttatt ttagaaatac tttttttgaa
     7621 tacgagtaat ataaataact ttatttactt atattttatt ttaatcctgt ttagtttgac
     7681 ttttttaaat tgttttaagt acaactaata ggtaaaatag gcatttagat tttccttttt
     7741 atcaacctat taaatactgt ttaatatatt ttttcccctt ctatttaatt cacttccctt
     7801 tctaccagat tcacatatta tcaaatccag ctcttttaac atattaagta ttccccttac
     7861 ctcaccttct gttatattgt agcctaattc atttattgaa ttaacaatac catttctacc
     7921 tatattttta ttaagattat tggcattttt taaacactcc attactaact ttactttctc
     7981 tatagatgtt ctatccttta atatacttat ttctttacta aaatcttcag ttatatttaa
     8041 taagttatga ggtaaatcat ttattaaaat aacatcctca gaactcataa tgttaatata
     8101 catagcagta ttttttaatt ctcttatatt acctggccat tcatatgcat taataatgtc
     8161 ttgaacatct tgactaattg ctctcttcct tttcataaaa tgctttaaca tagcttctat
     8221 atcttctttt cgctctctta aaggtggtat atttatgggc aatacattta tcctatagta
     8281 taaatctgct ctaaatctat ctccttttat catttctaat aaatttttat ttgtagccgc
     8341 aattactcta acatctatat ctattacatt ttctcctcca accctcatta cttgattctc
     8401 ttgtaatact ctaagtaatt tgctttgtaa gtatataggc atgtctccta tttcatccaa
     8461 aaatatagtt ccattgttag cctgttcaaa taatcctttt ttacctcctt ttaaagctcc
     8521 tgtaaaagca ccttcttcat atccaaaaag ttcactttca agtaaatttt ctggcatagc
     8581 tgcacagttt atcgcaataa atggctgact atgtcttggt gatgcattat gaatagactg
     8641 agccatgagt tcttttcccg ttccactttc tcccgttata agaattgtat aatcagattc
     8701 agctattttc atacctaatt tcttagttct ttccatcttc gttgaaactg tttttatatc
     8761 atcaaaagta tatctagcaa cttgtccctt ttctctaagc ttctttgata ggttttgctc
     8821 caattttctt atgtaagtta tttcttgaat acaaaaataa tatccagtaa cccttccaaa
     8881 actctctata ttatacttat ttacgtttaa gtacttatta ttaaatttta taacttcatc
     8941 atgtgcctta ctcaaactaa aaatcttttt aatattctca ttctctaatt catctatatt
     9001 tttccctata atattacttt gaatatctag tatatcccct aaactattat tgcaaataat
     9061 tatgtttccc ttatctgata ccattgccat acctaagttt gataaattta ttatggaatt
     9121 tagttcatct gcttttatag taagttcttt gtatttagta tttattcctg aatataaact
     9181 aattatttca tctgtatact taattagcct ttcagtaatt tctttatcat ccagtttgag
     9241 ttttgctatt atttgaataa acgttgaact atcaatatac ctatggccta aatcaataac
     9301 ttcttctata tattttggaa ctaaacttgc ctctcctggc gttatagcta tcttaatatt
     9361 tctatattct tcattgggat tgtatggtat aaaattcaga tgatttattc ctatattgta
     9421 taaattagat attgtttcca atacagtatg cttattatcg tttactacta atacatctat
     9481 tccttctggc aatgaaaata acttatatat atccttttgc tttatgctcc tatttatagt
     9541 tattatcttt gagacatctt ctacatattt ttttattttt aatactctat catctatcat
     9601 taccaatatt atgtcatctt ttattaattc cttctctgtt attttattta aataataatt
     9661 ttttatcatt actttatctt taaatatcat ttctagattt ttatttaaga aacattctaa
     9721 tttagacaac ccatcagtta ctactgctat tgttttcatt tgattactcc ttttaaaata
     9781 tactctactc ttttataata tacatttgat tttcacttta ataacacaaa aaattgtaca
     9841 tttagtatac taagatattc ctttattagt tttatgtcca taaatatcat agttgcaata
     9901 tctatcatat ttcttaaact tctataaagc aaaaattgtg ccaaattaat aattttctta
     9961 attttattct aaagttcctc aatagtattt acagacttag cacttatagc ttataagtct
    10021 ttatattatt aatctatttt tataggttaa ttgtacaaat catatatcac ttttacccat
    10081 taaatgcatt ataaaaaaga tggaataacg attcgtcact ccaccttttt tgtaataaaa
    10141 aattgtatat ataaacacta tttactactc ttctaagtac aaacattatc tacatactat
    10201 aacctttcac cattagattc tattacttgt ttataccagt caaaactctt tttcttaatc
    10261 ctagacaagt ctccacttcc atcatcatac ttatccacgt atataaaccc atatctcttg
    10321 gccatctcac cagtagatgc acttactaaa tctatacatc cccatggagt atatccaaat
    10381 aaatcaacac catcttctac agcttccttc atttgctcta tatgtgcttt taaatactct
    10441 atacgatagt catcatttat cactccatta gattcttttt tatcataagc tcctaaacca
    10501 ttttcaacta ccatcatagg tattctatat ctatcataaa tttcatttag tgcatatcta
    10561 agcccttcag ggtctatttg ccatccccat tcactagact taagatatgg atttttcact
    10621 ccacctaata tgttgccact tgtattatct tctttagaat cactacttac acatgtagac
    10681 atataataac taaatgtata aaaatctaca caaccttctt ttaaaatttc taaatctcct
    10741 ttttccattt ttatttcaat attattttct ccaaaatatc tattcatata agttggatat
    10801 tcgccacgca cttgtacatc tccacagaaa taattcatta ctctactatc tctttgagtc
    10861 ttaactgcat catctggatt acaagtataa ggatacattg ttgcaaatat ttgcatacat
    10921 ccaacttgat aatttgaatc tatttcatga gctaacttaa cagccttagc acttgctaca
    10981 aattgatgat gtaacccttg aaaacgaagt tgaggtatat ctacttggtt tataaaatct
    11041 gtagtccctt catttaatat tccttgtgat aagtagcctc ccataggcat tgtagctgaa
    11101 tttatttcat taaatgtcag ccaatatttt acttttcctt tatatctttt aaatattgtt
    11161 tcacagtaat tcatatacaa gtctattaca tctcttgaaa cccatccatt gtatttctct
    11221 gttattgcaa atggtatttc atagtgagat attgttatta atggttctat attatatttt
    11281 aaacattcat ctatcacatt ttcataaaat ttaagtcctg cttcatttgg ttctttctcc
    11341 attccagttg gaaatattct agtccatgct attgaaaatc taaatacctt aaatcccatt
    11401 tctgcaaata aagctatatc ttctttgtat ctgtgataaa aatctattgc ttcatgactt
    11461 ggataaaatg ttcctttttc tataactctc gttattcttt tacttacttt ttgagaacct
    11521 ccagtacaca tatctgaaca agaaggacct ttcccatcta aattccatgc tccttcacat
    11581 tgatttgctg ctgttgctcc accccataaa aaatcttttt taaatgacat acctatatcc
    11641 tcctattata attcttcacc atttgattct attacttttt tataccaggc aaaactcttc
    11701 tttttactgc gttttaaagt tccatttcca tcattatctt tatccacata tataaatcca
    11761 tagcgcttct tcatttctcc agtacttgca cttactaagt caatacatcc ccatggtgtg
    11821 tacccccata aatcaacacc atctaactca acagcatcta tcatggcttt tatatgagca
    11881 cgtaaataat caattctata atcatcattt atatttccat tttcatctgg attatctact
    11941 gctcctagtc cattttcaac tataaataat ggtttttgat aacggtcata taaagagttc
    12001 atagttattc taagccctaa tgggtctatt tgccatcccc actcacttgc atctagatat
    12061 ggatttttaa gacttgcaaa aacatttcct tctgttaaaa tatttacttt tgggtctgca
    12121 cttgtaagtc ttgatgagta atatgagaac gaaataaaat ctactgtgtt ctctttaagt
    12181 atctttttat catcttcttc catcactata tttattcctt ttctctccaa ttcttttaat
    12241 gcatatgttg gatattctcc acgagattgt acatctataa aaaagtaatt ttccctatct
    12301 ttttctatag atttccatac atctagtggg ttacaagtat taggataagt atttcctgct
    12361 gctaacatac accctatttt aaattcttga tttatgttat gtgctagttt agtaactaac
    12421 gcactcgcaa ctaattgatg atgtgcagct tggtatttta tttgttcttc attttctccc
    12481 tcttcaatat agagacctgc acctataaat ggtaaatgaa gtatcatgtt tatttcatta
    12541 aatgttagcc aatactttac cttaccttta aaccttgtaa aaataacttc acagtatttt
    12601 acatataagt ctactagctt acgatttctc catgctccat atttttttac caaatacatg
    12661 ggaacatcaa aatgacagat tgttactact ggttctattc catactttaa acactcatca
    12721 aataaatctt catagaattt tagtccctct tcatttggaa catcctcaac tcctgttgga
    12781 aatatcctag tccatgctat tgacatacga tagcatttaa atcccatctc agcaaataat
    12841 tttatatctt ctttataatt gtgatacata tctattgctt catgagctgg gtaatagtgt
    12901 tcatcatcac attcaaacat tttcattttc ccagccaaaa ctggaaatct atcttttcct
    12961 tgcggtgtaa cgtcaacatt agctagacct ctaccatctt ctttatatcc accttcacat
    13021 tgatttgctg ctgttgctcc accccataaa aaatcttttc taagtgccat aattctacct
    13081 ccaataattt atttatagta actttatttt aaaataaaaa tacatatata taattcttaa
    13141 ttaatagaac tctattttac gctaaaacag ttatcaattt ctgactatta tttacatgct
    13201 tttgtgtatt ttctacaata tccacataat cagaataatt agtaacaact actggtgttt
    13261 ctaagcaata tccttctttt gaaatacttt ctatgtcaaa agttattaat gtctgacctt
    13321 ttttaatctt atccccttga ttaacaaaag atttgaaata tttaccttcc aactgaatag
    13381 tatttaaccc tatatgtatc aatagttcac aaccattatc agatataatt cctattgcat
    13441 gttttgttgg aaataaagtc attacagtcc catcaaatgg tgccactact tttccttctt
    13501 taggtgttat tgctacacct tttcccaata tacctgttga aaacgctgca tcttgtatct
    13561 gctccaaagc ctttacttca ccttttattg ggctatatac tacttctctt ttgttttttt
    13621 ctataacgcc ttgcccatta tcatctttag aaatttctac tatagcatca tctttccaaa
    13681 aaaataatgt taaaacaaat cctactacaa tagctactat tattgctatt atagttgcat
    13741 aaacaccttt catatctcca gttgttggat ttatataatt aggtatacca aatattccaa
    13801 gtccacccat tacataactt tttacattca ttatccccat tacagctcct gccacagcac
    13861 cacctacaca actatatatg aatggtgttt ttcttggtaa agttattcca tatattgcag
    13921 gctctgttac accacatatc cctgaaataa ctgcaggtat acacagctct tttatcttct
    13981 tatcttttaa tttaaagtac attgctgcta ctactgctgt ttgtgcaaaa gatggtacaa
    14041 atgctcccac taatattgtg tcaaacccca tagtcataag gtttgacata gctattggta
    14101 ctaatcccca atgtagacca aatattacaa gcacttgcca caataaacca gttaatgcac
    14161 cacataaaat tggtgaaaag ttaaataaag ctaaaaatcc atttccaact atattagaag
    14221 cataggtagc tataggccct ataaccataa atcccaatgg taaagaaact aataaaactg
    14281 ccattggtac aaagaaattc tttactacat ctggtattat tttagcaaac actttctcca
    14341 acttagctgc taataatact attaaaatta ctggtactac tgttgaagtg tagttcattg
    14401 ctattactgg taatcctaag aaagttatat atactggtga ttcaaatatt gttccagtaa
    14461 atagattgta taaaggttga cctgtagatg ctagagtatc taattgaata cctgaatagc
    14521 ataaaactgc acctataagc attccaataa atggttttaa tttgaatttc ttagcagctg
    14581 tatatccaag aattattggc atatactgga acattgcatc tccaattgca tttaacataa
    14641 tatatgcacc atcagtttct ccatacaagc ccaatgctgt aaatactgca ttaaatcctt
    14701 ttatcatacc agcagcacac agaactccta atattggttg aaaaactcct gatattatgt
    14761 ctataaactt attaagtaat ccttttggcg catctgagtc tgaattatct attgtatctc
    14821 cccctaatcc agccaaatcg cacacaactt cataaacatc tggaacatga ttaccaataa
    14881 ctacttggta ttgacctgca cttttcatta cagtaactat accatccatt ttttttagca
    14941 catcatcatt tgctattgac tcatctttta atttaaaacg tagcctcgtt atacagtgag
    15001 ataaactgct aatattatcc tttcctccta cattcgcaat gatttctttt gatagttgtt
    15061 catactttct cattttttct cctccatttt ctttttgaaa tatgaaggtt tggccaactt
    15121 aatgtcacca cccaatatat ataaacctaa ttattaggat ttatatctga cttaatactt
    15181 ttacttacca ctctttcaac gtgaatagtt aagtataatt tttcctcatc tgttaaatcg
    15241 tagttgtatt ttttatgtat aaattcatcc aacttttcta tgaatttata agcattttta
    15301 tatttaactt ttattacatt taataaatca tcttcattat tttcatgtaa attattacta
    15361 acaattcgtt ctgcaaaata ctttaagtga gtaataaatc tatgataata aactgaatct
    15421 tcatcaaata ctattctgta atggtattta accatatttg aaatttcttg tattatttgt
    15481 gtaatctcat atatagtttt tatactttga tccatttgtg cattaactat atgcagtgct
    15541 ataaatccag cttcatcttc tgatagtcta atttcaaatt ttttttctat ataatccaat
    15601 gcctctaacc ctattttaaa ttcactctta taaaatcttt ttatatccca aagtaataca
    15661 ttttttacac ttactccttc acgagctctt tctattgcag tatacatatg gtctgttaaa
    15721 gaaatataaa tactatcatt taatcttttc cctagttttt ctttagcata taaaattatt
    15781 tcatctgata attccatata tcttatagga atatctgcta ttagttcttt aaatttatca
    15841 gatatatttg ggtctgaaag tttgaatatc ttatcaattt tatcctttga tatatgctct
    15901 cctgtacgtt ttttataagc taaacctctc cccataacta ctatttcctt atgattttca
    15961 tcagttgtaa taattacatt attattgagt attttttcaa taatcattgt gatgccccct
    16021 ttattagcat taagttaaaa taaatctttt ttaattattt agaatattta aaatgtaatt
    16081 tattacataa aaaaaaacct atttattaca cattaaatag actcccatga gtcctgagta
    16141 ataaataggt ttagcttgta tttaaacaat cactatccgt ttgtatttta ttttaattat
    16201 tatttattat atgttagcaa atttattttt tagtgtcaac tttttctcaa tattttaatt
    16261 ttaaataaat tatataaatt gaatcttcaa agtactattt ttatcaataa acaaaataaa
    16321 aaaggtatta ctttctttaa gtaataccca tttattttta atttaagtgg tttttcttaa
    16381 tctcatcaag tagtccatct tctaaataaa tttctaatgc acatcctacc attgccttta
    16441 ctgctttgtg aagtttatca taagcttcct cagaatttgc ataatttaga aattcttttt
    16501 catgaacata taccattgga cttatatcta aggctatttc tgtatacata gtagggcaaa
    16561 cttgacttac attacctata tctgtagaac ctactggacc atctttacct tctaatatat
    16621 ctgttatacc tgcctcaatc aaattattct ttgttatctt ttgaagtact ttgttattta
    16681 ttatgttgtc aaaagaattt tcaaagtatc tataagaaag cttagctcct gtcattaact
    16741 ctgcaccttt tgcacagttt ataacttttt tagttacttc atctaaataa cttctttcag
    16801 cagctcttac gaaaaactta caagctgctt tttctggaac tatatttggg gcctctcctc
    16861 catttgaaac tataccatgt attctaacat ctgattttac atgttgtctt agtgcattta
    16921 tcccagcaaa agtaagttgt actgcatcta gtgcattgac tcccatatga gggtgactag
    16981 ctgcatgtgc tgatacccct tcaaaactaa actctaatga atctatcgcc aaagccttac
    17041 agtttatatt attaaaagct tctatatgca tttgataact tacatcaaca tcatcaaaag
    17101 ctcctgcctt aactaaatca cattttcctc ctgttgtttc ttcagctggt gttcctatta
    17161 gtacaatttt tcctttaaaa ttttctttta acttacttaa tactatacaa gctcctagtg
    17221 tagatgctgc aatccagtta tgtccacaag catgagctgg ttctttattt tctccatatc
    17281 caggaagtgc atcatactca gctaaaaatg ctatggtagg tccatcatta tctcctaatt
    17341 cagctctaaa agcagtctcc atattacaat atggataaac tgtatcaaat ccatattcct
    17401 tcattttttc tactaagtat tttgatgata tgtattcttc ttcccctaat tcaggattgt
    17461 taaatataaa attacaaact tcttcaattt cttttccaag ttcattctct atctcataag
    17521 ctctcttctt tatactctct atcataattt acctcctact agaatcctag atataatcct
    17581 attgtcaaaa atgccattac aagaattgtt aatactacca ttaaaggaat tacgaatcta
    17641 acccactttg accaaggaat attggctact gctagtgctc ccattaaaac acctgatgta
    17701 ggtgttatca agttagttac acctatacca aattgataag ctgtaactat catttgtctt
    17761 gatacatcta ctaagtcgcc aagtggtgca agtacaggta ttgtaagtga tgcaagccct
    17821 gatgctgatg gtattaaaaa tgctattaat ccttctatta agaaagttaa atttataaat
    17881 atagtttttg gtaatccatt taataaattt gctgcagaat ttaaaattgt atctattata
    17941 aatccatttt ctgctattat tacgattcca cgtgcaaatg ctatacacaa tcctgctgta
    18001 attaaatctc ctgctccact tataaatgaa ttaactatct catcttgttt aagccctgat
    18061 gctattcctg atattatacc tatagctgta aatatcattg caatttcagt tatgtaccat
    18121 tctagtctta aaactccata tatcataatc gccataccta ttgtgaaaat ggctattacc
    18181 atggcttctc tttttgtaaa ttcctttata tttgtatcac tattaaccat taattcttga
    18241 tttagtgata tatcatgtac taaagatttt ttagggtctt ttttaacttt attggcatac
    18301 atcataacaa atcctataga tatagccata tatattataa acataacaac tctaaactct
    18361 attccagaac ctggtactat ttgtgataat gcttgtgcta ttcctacaga gaaaggattc
    18421 gtagtagctg ctccaaatcc agctgttgct cctataaata ctgtggccac tgctgtcaat
    18481 gaatcatatc ccatactggt cataagtgga ataaatatca tataaaaagg tagtgtctct
    18541 tcactcattc caaacgtcgt cccacctagt ccaaatagaa tcattgaaat cggtattatt
    18601 aaaaattgaa acttcttcat tttatttaca gtatgagcta taacagcttc tatagctcct
    18661 gttttattaa ctatcccaaa agctcctcca actattaaaa caaatccaac tacttcagct
    18721 gcatctataa acccgtttat aggtgctgta aatacatcag taattccttg tggatttgat
    18781 ggtgcttttt cataagttcc agctacaaca actgaccttc cgtctatatc tactctttca
    18841 aaatttccac ttggcactat ccaagtaaga attgccatta acactattag caaaaatatt
    18901 attgtaagtg tctgtggcat agtaaacttt ttctttttta aactgctcat ttttaatctc
    18961 cccacttttt aaattaaata ttttattatt gcatcccttt atcctatttt gtatagatgt
    19021 aaaaaagctc aacatggata catgttgagc taaatgtgct aaatacaaca tacctgcatc
    19081 ttccctttat cttgtttaag atagctctcc atcaaaattc gggataattt tgatgacagt
    19141 actgtaccta ttaaatacag tcccagcact agcttcggac actagcactt cggcacttac
    19201 tccttttata ttgcctcatt ggtttccaac ctcttcaata ttaatcttag taaatgcaac
    19261 ctctacctca atctgtttgt tagatgaggt aattcttatt aatttttatt aattcaaagt
    19321 aattgtagca taatatgtcg agattgtaaa tattttcagt ataataattc ttactttatg
    19381 atttcattcc atttttcaca aataattttt tctccatata atttaaatac tgtagtatat
    19441 tttaaataca cacaaacttt ccacaaattt taaattatta taataccttt ataataaaaa
    19501 ataaaagcta tgtaaactta tcaatttaca tagctttata tacattaatc tactccaagt
    19561 acagctttaa gctttgatgc tattgtaata actcgtggac caaatataac ttgaactgag
    19621 tttttctttt taacaactcc acttgcaccc aattcattta cccagaagcc atcttcatag
    19681 aatccatcaa ttgaatttag ctctacccta agtctagttg cacagtttgt aacatttttt
    19741 atattattac ttcctcctaa accttctatt atagaatgta caagctttgt atctttatca
    19801 tctacttcct cttctactgc attgttgtat gctgttttac cagcatctac agattgtctt
    19861 tctctatatt ctttcttact ataaagctta acttcttctg aacttcttcc tggtgttttt
    19921 atatcaaact tcttgattgc ccatgtaaat attatataag taactataaa aaatattggt
    19981 atcaaaaata ataatggtaa cgcatccact ttttgtggtt gtaataaatt aggaaacata
    20041 tttctaaatg attctcccat tatagaaaca ttaaacattt ctgtaaatac atacgtcaat
    20101 cctgccagtg gtacatatgc tacaaaatac aataatggtg caacaaatag gaatgtatac
    20161 tctattggtt ccgttacccc taccaacatg gctgttacta ttgctggtat caatatactt
    20221 attgcttttt tcttattagc attatcagcc aacttataca tagctaaagc tgctgctggt
    20281 aatattgcaa aatgtattaa taaacgacct gttgtgaagt tacgagttat ataagatagt
    20341 gtatctgggt ctcccatttg agctaatctt atattattaa caccctcgta aattgctcca
    20401 cctacttcca ttgttccacc tactcttgta tattctatag ggaatgcaat taagtgatgt
    20461 ataccaaatg gtaataatgc tttatccatt gctccaaaag taaatgttcc taataatcca
    20521 ctagaaccta tccaattacc aagtttagat aatgccaacc ctactggtgg ccatataaag
    20581 tacattgctg tacccaatac tattgatgct ataaacatca taatcattga aaatcttgga
    20641 cctgagaaaa aagataatgc actaggaagt tctatgtcaa caaatttgtt atgtactgca
    20701 ctagctacaa atccagctat tatagctcca aatatactac aatcatatga aaagattcct
    20761 aagaaagaac catataaaga acttcgacta tatgcttctg cctctcctaa tccagatgcc
    20821 ataaacgctt ctgctgtaac tgtatctgaa tttataccat ttgctgctaa caatgtactt
    20881 ataactgtgt gcattcctat aaataaaaca attcctccta atgcacccca tcctttttcc
    20941 tttttagata atccaaaggc tatacctgta acaaagaata ttggtaagta tctcattacc
    21001 ataaatccta aatcgtttat tactttaaag aatacattta taaatgaccc ttcttttatt
    21061 agtccgctaa gccctaatgc agaaacgttt tccatatttg caaaagctga ccctaatcct
    21121 ataaatactc ctgctacaac aagtaatata acaggtatca tcattgctcc agaaagtact
    21181 tgtaatttat ccttcatagt attatttcct ccatttttat tgaaatgcca ggctatataa
    21241 tgcatatata tatacttcta ttgctttgta taatgtatct atacttatat tttcatttgc
    21301 ctgatgtgaa tttggattat ccccctctat tgtgggtcca aatgcaactc cattttttaa
    21361 cactcttgca taagatgcag ctccttttgt aaaaagttcg gcttcttttc caaaaccttg
    21421 tttatatgca tttgataatt tatttattaa ttcactatct ggttttacat acagtaattt
    21481 taaatcctta tatatatcaa ctattaaatt ttcttttttt ccaatttcat taattctatt
    21541 agttataaac tcccaactta tacccttagg atatctaaag tctattttca tattcaattc
    21601 atttttctct aagaagatac tcattataca tattgtagaa actcccatat ctacatcttc
    21661 tttatacatc cctaattttt ttccatgatt atcatctgta aagtatgaat ttaaaatatc
    21721 ccttattata acgcctttat catttagttt tttaattttc tctaaatctt ttgctaaact
    21781 aaatgcacag ttatatgacc tgtgtggatt tctcgataaa gccctttctc ctgtatatct
    21841 tactaatact ttaccttcat ctaattcttc tatctctgta taataagcta atgattctac
    21901 taaatctttc ttatcattag ttttaaaaac agcctcaatt ttgtcacata caaatccatc
    21961 ttctttgttt gactttatat caactaaatt gtgatttcta aatttatctt tatctatttt
    22021 ctttcttaga ttgaagtata aaataccttt ttctccattt actattggga agtttccatc
    22081 tggagaaaat gcaaattttg gctgttcatt gacactaaag taatgctcca tacattccca
    22141 agtcgtctct tcagctccac ccactatcaa tctaatcttt ctcttcggct tttcattaag
    22201 ttctcttaag aaaagtaatg catataaaat ccctatcaaa ggtcctttgt tgtcattaac
    22261 tcctctacca taaagatagt tatctttttg acatacttta aaaggtttac tcttccaaag
    22321 ttctttatta tatattggca ccacatctat atgattaagc actcctacta cttcttctcc
    22381 ttctccatat tctatatgaa ttgcatatcc atcaaagtct tttacaacaa aatctttgtc
    22441 ttttgctatt tgaataagct tatcgaaggc atttcttatt tctatcccaa atggttgatt
    22501 tatatctgtt gtactttcat ctctaattga aggtatttca actaaacttc taatatcatt
    22561 tatgatattt tcttgattgg attttaaata atcaataatt agttctttgt ttactatcat
    22621 cttaattatt tatcacccct ttatattaat tttacaaagc atttcagttc atatctacat
    22681 tataaatttt gaatattatt ttttcaatat ttcaaatact tctctaagtt accataatga
    22741 tacggtaaac aatcaacaga ttatgacaaa atcatactat acctattact aatattttaa
    22801 cttatctatt acgtcttttt taatgtaagt ttattccaaa aatacataaa aaaagatgta
    22861 gccttagcta catcttctta tatctattca acttccatta ttatgctatt aatatctaca
    22921 tttccaggtt ctatcctatc ttttactgtt ctcttaacag aatcagcatc atccattata
    22981 actacaggag ttattatatt taaatcattt gacgttataa aatctaaatc tacttccatt
    23041 attaaatctt tagcttttac cttatctcct gcctttacct taacatcaaa cggctttcct
    23101 tctagtgaca ttgtctctaa acctacatgt attaatatat ttgtattatc attactctta
    23161 atcattattg catgctttgt atcaaataca ttgactattt caccatcaac aggagaatat
    23221 aacttacctt cacttggtat tattgctact ccatctccca tcatcttttg ggcaaaaact
    23281 gcatctggaa catcttctaa gttcactaaa tttcccttaa aagggctata tattttctca
    23341 atcttatttt cttttttctt aaatatatta aacataacac actctccaca ttataaaatt
    23401 ttatgataat atttttctta aagcttttgc tacaccatca tttacattag tatcagttac
    23461 aaaatctgcc atagatttta cttcatctgt agcatttccc atagcaatac caagacctgc
    23521 aaatttaatc atggatatat cattttcatt atcacctata caaataactt ctttgctatc
    23581 tatacctaat atatcacaaa attttttaac tgcaatacct tttgaagtac ctttactcat
    23641 aacttcaaag tttatctttc cagaacttac tacttctaaa tcttcttgtt ttttaaattc
    23701 ttcctttgct tctctcaaag aatctacgtt ttctgagaaa agtatacatt ttaaaatttc
    23761 attatcagct ttttctaata aagtttcaca acttgcattt tctattattt caattctgtc
    23821 ttcttcttta tttttactat ttattaatct atatggatgt tcttcaccta tttcattgtt
    23881 ggatataact attttacagc ctttaaaatg tttgtttaaa ttatactttt cacctattgt
    23941 atagattttc tttagagctt ctttgctcaa aaccttctta tactcatatc ccagtaattt
    24001 aaaatatgtc ccattagttg atataatata tatatcatcc cctaaaacac ttgtaaaata
    24061 tttcatagca ttataaggtc ttcccgtaac taaggctacc ttgataccct tttccatggc
    24121 ttcttttagt gctttaatat tttcttctga tacttcaaat cctttaccca tcaaagtacc
    24181 atccatatca gtacaaatta atttatatcg catttgtatc acatcctagt aagatttttc
    24241 taatatttca acaaggtctt ctttctttcc aaaaggtaaa aatcctaaaa ctggacagct
    24301 tgttatttgt tctaattgtt cctttgttac attaaactca gacatttttg tcttaagccc
    24361 cactctcttt aaaaaacttt ctatttcatt tcctaaagtt ttagctgctt catcatcttc
    24421 cactttttct aattcttggt ttagtattct cgctactttg gcaaatttct ctatattatc
    24481 cttatagaac ttttctgcaa attttgggaa cactatagcc aaagcctcac catgaggaat
    24541 atgtgtaact cctcctatta tttcacttag tggatgtggt gctgctgctc cagcatttgc
    24601 cagtgaactt cctcctaatg tatctgctac tgctaacttt gttctgtatt ctatattatt
    24661 tttatcttcc attactttag gtagatagtt aatgactaat tccatagatt tttcagacat
    24721 tatctctgta aaatcattag catttggatt tatataactt tcaaatgcat gagtaaaagc
    24781 atcaaatgct gttgatgcag taattctctc tggcagagtt aacataagct ctgggtcaac
    24841 tatacattct tttgagaaac aattttgatg aaatattgta tttttctcgc ttcctctcga
    24901 tattactgct gcttgtgtaa cttggcttcc tgttccagaa gtagttgata cagctattaa
    24961 tggtaattct ttttcactta ccttatcgta tattttaaat ggatttgtat atgtactaaa
    25021 catataatcc caatctattt tttctaaacc aaaagtcaat gctaatactt ttgcagtatc
    25081 tatacttgaa cctcctccaa cagctaaaac aacatctaca gattcttttt tagctaattc
    25141 aaacccttca tttattaatt ctacagttgg gtttggctct actctatcaa agtgtacaac
    25201 ttctatacct ttttctatta aattcaatgt aactctttta tataacttgt ctaatggctc
    25261 tacatttgga gtagtaacta acaaacattt ctttccatat tttcctatta ttctaggtaa
    25321 ttcacttaaa gttccttgtc caaaatttat tcttgatggt tgataaaagt taatcatatt
    25381 tttcctccct taaattattg ccttcatatt caaattatac atatcatagg atttttaagg
    25441 caataccttc actgttaact cttatttact aaagcttatg gcaattatta gacaataaat
    25501 gttttattat taccatatca ttatgtaaat tttattaagt acccatgtta taattttata
    25561 atcaatctta ttgtataatt gacttaataa taaaaataga gtatatacac ttttttatga
    25621 caaatagctt aaaggagaat taaattatga aatatttgat agacagtgct aatcttgatg
    25681 aaattcaaaa gtgtctaaag tatggattta agggaataac cgctaatcca tctatgtatc
    25741 ttaaagagaa agtaaacttc catgaattta ttgagacatg tgctaattta aatccagaaa
    25801 tattaactgc tgaagtcatt ggaaataatt tatacgaatt aattaattca tgtgataaga
    25861 tattaaatat aaataaaaac actataataa aaataaattt ctctgaagtt ggcttagaac
    25921 ttataaacat attaaatagt aaaggtgtaa aaactgcatg taccttgatt ttcaatgtaa
    25981 atcaagcatc attagctata aatgcaggtg ctgattacct attccctttt ataggtagaa
    26041 atgatgaaaa tgggaatgat ggtataaaaa accttacaga aatatgtaac cttatatcaa
    26101 taaatggata taatactaaa gttgttgcag cttctattaa aaatgtattc cacttaactc
    26161 aagcatctct ttgtggatgt gattatgcag ctgtaacata tgatttattt aaaaaagctt
    26221 cttttaacca gcttacaata gatggtgcca atacttttga aagagattgg tccaaggtta
    26281 ataaatagta actaagattt aggagggttt gcattgaaca aaagattatt agatattgct
    26341 gaattattac tcaacagctc agactatatt actgtagata ctattgccga agaattaaaa
    26401 gttagtaata aaactattcg taatgacctt gttattttag atgaatggct tttagagttt
    26461 aatttatccc tagataaaaa aactggttca ggggttatta ttctaggtaa tgaagatata
    26521 aaacttaaag ttattagaga tatcaatgat aaatcaaatt gtatatatgc atactctcct
    26581 gaagatagaa aaaggtatat tctaagtaaa ttatttatta aaaattctaa gtttaggatt
    26641 cgtgatattt gcaatgaact acatgttagt agagctactg tgcataaaga cttggtagtt
    26701 attcaaaatt ttttgcaaaa ctttaaagtg actctagtta gaaaaaccaa taatggagta
    26761 tatttagagg gaaaagagaa agatattcga aaagcaattt ttgaacttgc tacagttaat
    26821 aaaagttata ctgaacttaa agaaatatta ttctcgaaat ctaatgaatg caaagatacc
    26881 ccatcaacaa aaatatttaa ggaactattt aactatgatt ttgagaaatt atctcaaatc
    26941 actctacaca ccttacaagt agaaaaaagt aaccttagtg atgagtatta tataaacttc
    27001 ttaatacata tagctatatg tattaaaaga atttcaatta aaagatatat taacttatct
    27061 gaaggttttt ttagagaatt attagaacat gaatactttg aaaaatcaaa aattttatgt
    27121 gctaacctaa gtgaagcttt tgacgtagaa tttattgatg aagaggtctg ttatatcctt
    27181 cttcatatag aaggattact taaatcaata aagaaaacta ctactcttga acctcttaaa
    27241 aatgaagaaa ttacagagta taatctatct gaagcaatag ctgttcattg gggaagtata
    27301 ctaaacttaa acttaaagga tgatttaata ttaataaagt ctcttgctaa tcaccttaaa
    27361 tcagttctac acagaataaa ctatgggttt actattacaa atcccatatt aaatgatata
    27421 aagagaacat ttccttatac ttataaggct gctaaagagt ctaatactgt tatcaaacag
    27481 ttgatgaatt atgaagtcaa tgatgacgaa ataggatatc ttgctttata tttaatatca
    27541 gcaatagata gaagcaaatc tcccctaaat actattttaa tatgtcactg tagtgatggt
    27601 gtatctaatt tattagtcca aaagttaagt tttgaattta atcagattaa tatagttaaa
    27661 tctatacctc tatcttctat ttcatttacg aactttgatg atgtggactt aatactaacc
    27721 actgctcctg tagactttga acataatgct gaactgataa atataaatgc tcttttaagt
    27781 aagaatgata tttctagact tagtacaatt ataaaaaaac tatattctga aaaaaataaa
    27841 atattatctt ataaataaac aaaaataggc ttgtctcaac atagagataa acctattttt
    27901 cattgtccaa atgataaaaa tatatttgat ttcatagaaa aagtgcctca tgaactaaaa
    27961 aattcatttt gagacacctt cattttttat atttaatctt tttatttaag cttttatatt
    28021 ttttaaactt ttatatttaa agtaaatttt attattttta ttgagcacaa gcattattta
    28081 tattattaat ttcttcagta ttcttaccaa actttgtagt tacaaatcct attgttttta
    28141 ttatgagtaa taaagatatc aatgaatata tagatatcat aaaattctta cttattattc
    28201 cagacgctac tattataaat ccatctagca taaataatat aactggcaac ttcagaaact
    28261 taattacctt attaataagc attgcaacta aatcagttcc accagtacta cagcccctta
    28321 ttattgctat ccctatagca gttcctacca ataacccacc tatcactcct gctactaata
    28381 gattgtcagt tatgtgagtt tgtggtatta cttttaagaa taatgggccc ataagagtta
    28441 tatataatgt ttttattcca aaacctttcc ctaaaaataa taatcccatt actaaaagtg
    28501 gcatagatat tcctaatgat atattctcaa caggcattga taagaatgtg ctaagtacga
    28561 ctgataatcc tgttatccct cctggagcta atgtatgttc tgcaaagaaa aaattaactg
    28621 ctaatgccat taaaaatgct gatattgtta tcagtaaata atcttttatc atttcttttt
    28681 tcatttaata acctcctatg attatattgt ttcagtataa aaaatcaatt aaattagtct
    28741 agcttcctgt agtttttcat aaaattaatg cttccttaaa acattaagaa agcatctctt
    28801 ttatcatagt ttattatctt attctaaacc aaactttgta gctgcttcat ctatagcttt
    28861 tctacaattc ttaacacctg ctgttatacc atctttatcc ttgaatattg ctgaagataa
    28921 tattacaacg tctggattac attctattaa tggctccata ttgtctgctc ttagtccacc
    28981 atctattgct aattcacact taggattctt ttcgtctatc atttttctag ctctttttac
    29041 taaatcaaca gcactctttc tccatcccca gttatctttt ccatcagttt catcaacacc
    29101 atgaacgact atttgcaatc tatctatatc atatatagct tcatcaacaa aacataatgg
    29161 tgtgtaacat ccaattgtta atcctacttt cataccaaat tctctacagt aatttattat
    29221 gtatgcaaga ggagcaccta aaaaatgttc tgctggtatt atcaacaagt tacatcctgc
    29281 tgctgctatt ttttctataa ataatctatc acaatctctt gtgtatatat gacattctat
    29341 tggtttatct gtcactggtc ttattgcttc tattatttga tgaccaccca taagttgcat
    29401 attttttaag tcatgcatat ctgctgcatc tgaatgtacg tagtctacac ctgcatccat
    29461 tgcttcttta actacggctg atatatgacc gtaatttaca tgtgctaatc ctgctgctat
    29521 ttttatgtgt ttcatatttg tatacctcca tattttactt taattagtat taaatattag
    29581 tttttgatat ttttttaata ttttttaatc cagtattact tttatttagc attactttta
    29641 ttgctgtata tatttctttt atgaacttat tctgcttttg ttaactgtga acttattttg
    29701 ctttgtaaat taatattaca ctgatatatt ttttttcaca acactttgac tactccttaa
    29761 aagtaccttt atattgtggt aaattataga cattaattgt atataaaaag ttagtttttt
    29821 accacattct tatggcattt attgtctata ctaactcaat tgatatagct agttcattta
    29881 atatataatc agactataaa tataaattta ctcgataaaa agtaccattc ataacaatat
    29941 taatgaaagg gagatgtaaa atgagaaact gtattattgc ccaatctgga ggccctacag
    30001 ctgttatcaa tgcttctgca gttggtattt atgaaaaaaa tttagaaact aaatactttg
    30061 ataaagtata ttttggtatc aatgggattg aaggtatatt aaacaaaaat ataatagatt
    30121 cagactcctt aaacaaagat aatgtattaa atttaaagca aacaccttcg tctggtcttg
    30181 gttcttgtag atacaaactg gctgattata aaataaattc ttatgattat gatagagtta
    30241 taaatatact agatgaatat aaaatagata cattatttta tataggcgga aatgattcta
    30301 tggacacagt gatgaaattt tctcaatact gcaaaaacac tacttgtaaa ataaaattta
    30361 tagggattcc taaaacaata gataatgatt tattaggaac tgacaactgt cctggatttg
    30421 gttctgctgc aaagcttatt tctacactaa taatggaaac atacttagat ttttctatat
    30481 acaaaaataa tggtatattt atagtagaaa ctatgggtag agatactgga tggttagcag
    30541 cttcttcttc actagctaga cttaatgata ctccacttgt tgatttaata taccttccag
    30601 aaattgattt ctatgaagat aagtttatag aagatgttag aaaaatattt aaagaaaaaa
    30661 ataaggctta tatagttgca tcagaaggta taagggatca atacggcaac tttatatgcg
    30721 aacaaaaaac ttctgctcat gataaatttg gtcattccca attaggtgga gtaagcaact
    30781 atctaaagaa tcttataata tcatcatcaa ttacaacaag agtaaaaacc ttagaacttg
    30841 gtgtaataca aagatgtgct atgcatttag cttcagatac tgatttaaaa caagcagctg
    30901 aagttggtgc atatgggata gaactatcta aaaaaggata tacaggagtt atggctatca
    30961 ttaatagaac atgcaataat ccttataagt atgaagttag tcatactgat atttctaaaa
    31021 tagctaacaa gacaaaatat tttcctaaag agtggataaa tgaagaatgt aattatataa
    31081 ctaatgaagc cctaaattat ctatatccac ttattcaagg taaaccagat atcaaatata
    31141 aaaatggact accttatttt tcaattattt actaggaaat tattataatt tgatatacac
    31201 tcaattctat cgctcttaaa tacctaagtg gctgttaaat aaaattataa attgttatat
    31261 gacagtcact taatcgttta attatttaac tgtttagttt tttaattttt taatcttttt
    31321 aatactaata caatttgttg taataatatc tcacacccat tttcattttg gatagattat
    31381 aattagttag acaataaata ttttgtcatg atattattat atatagtgat acaaatagaa
    31441 atacttttat catggctaga aaaataaaga ttacacaaag gatttaaaag tcaaacaact
    31501 tcaattaaaa taaatggtta aaataacttc taaatcaact acataaacaa taaaagaaca
    31561 taaagtagtt aaatctacaa ttataaaatt agtaagacta taataactta aataaaatta
    31621 aagttaacaa agactattaa agttagcaaa tattcctcta cctaaataat tgctttttat
    31681 aaagagttat ttattttaat tgaatttaat tgaaatacga ttttaactag aacaatagat
    31741 taatgtaata tttaatataa aataattata ataattgaaa aggaagtaat ctatgatgaa
    31801 aatagcagtc ataacaaccg aatttttaaa agagtttgta gataattcta taaaaaagtt
    31861 aaatattaat gcagaaattg aaatatatat ctatagagat ttttctcatg ttggagattt
    31921 atatttagaa atagaagata ggtttgatgg atttgcagtt agtggcccca tccccaaaaa
    31981 agctattaca aaaaaggctg gaactattaa aaaacctcta gttgattttg gtactgactt
    32041 acaaagttac tatgatatgt ttctaaagct tatctttaaa tataatacat tagatttttc
    32101 tagagcgtac tttgacttaa cagactgggc agataatcca aaagatatat catattattt
    32161 atcaaatggc acatttggag acttaatgga tagtattgat gaaaatgcat ctaaaatgag
    32221 tttatctgac atagcaaaac ttgaaaaaaa gattgcaaaa aaacatatta atctatggaa
    32281 tgaagggaaa atcgattttt ctactactcg ttttagtagt attgttccta tgttacaaaa
    32341 tgcaggagta aactttcatt tcatataccc tgatatagat ttattaaaaa atacttttaa
    32401 taacttaata aaagatattc atttacatag aatgaaggga aatcaaccaa gtgtaatata
    32461 tataacagtt aaagatacaa attatgatga ctctactcta gaccaagttt ataaaatatt
    32521 aaatagtatt aaaaaagata aattaaccga ttttataatt gaaaaaaata gtaattcttt
    32581 taaaattttc actaaatgtg aaacgattaa atcattaact aaaaatttta ctgcatgtta
    32641 tataaaagaa tctattgaaa atagtacaaa tattaaagta tgtgttggat atggaattgg
    32701 taatgatata aagcaagcta tgtctagtgc cataagtgct aataaggaat ctaaaataaa
    32761 tcctttaaat tacagtttct tagttaacga aaattatgag acaataggac ctttactgtg
    32821 tgataagtta cttactgttt caaataaagt aactccacat ataaatacaa ttgccaaaag
    32881 ttctaaattg tctactctta ctattcaaaa gattatatct gttactgaaa ttctacaaag
    32941 agatgaagtt actcctaagg aattatcttc atgtttaaat gtaacagtta gaactgctaa
    33001 tagatttcta agtagtctct taaagtctgg aaacgctact atattatatg aaaaacaaaa
    33061 ctcaacaaaa ggtagacctg aaagggtata caaattattt atatcagaaa ataaatatta
    33121 aaatccaaaa tgcataaaaa tattttatca tctaatatta agtaaaatta tatatgactt
    33181 tctaaaaggt atatctttgc atctttttaa gatatacctt ttatgatatt taataatatt
    33241 taataatatt ttatgttatt atctactaaa attattttct aaccatttta ctgcacaatg
    33301 agcaaataaa gctgaaccat aaactaatcc ctcttcatta aaaattgctt taggattatg
    33361 cataccaaaa ttatatccct gactaatact tcccattcca atccataaaa atactcctgg
    33421 aacccttagt aaaatctctg aaaaatcttc tgaacccatt gcagatggaa agtctattac
    33481 attttcattt cctataactt ctttaacata agatacaatc tcatctccta catccaaatc
    33541 atttataact gcaggagtcc ccattttaaa ttccacttta gcatttcctc caaatgattt
    33601 agctattcct gtagatattt caataattct attttttaat ttttctctaa caaattggtc
    33661 aaatgttctt aaagtacctg acatagatgc tttatttggt atagcatttg gtacgcttcc
    33721 tgcattaaac ataccaatag ttaaaactac aggttctttc tgaggttttt ctcttgaaag
    33781 tatagtgttt aatgctattt gtatatgcga cattatgttt attgggtcca ctccaaattc
    33841 agggcttgct ccatgagttg cttttccttc tactgttatt tcaaatatat ccgcagatgc
    33901 catagctgga ccacgtgaat aagctacttg acctgtgtta agaaatttac ccaccatact
    33961 gtgaaatgcc atagctgcat cgacctgagg attttctaat ataccatcat caatcattga
    34021 ttttgcacct ctggtagtct cttcatctgg ttcaaataca aactttactc tacctttaag
    34081 taagtgctta ttatccataa gtattttagc tgctcctaag agcattgctg tgtgattatc
    34141 gtgtccacaa ctatgagatg catctttatt cttactttta aaaggtaaat ccgtctcctc
    34201 cataatagga agtgcatcca tatctgccct taataataag gtctttccac cattttcact
    34261 gtctaaaaca gctactattc cattattaga cgtcaatttc ggagaatatc ccattttttc
    34321 taaagtactg gtaacaaagg ttactgtttt cggtagattg caaccaattt cgggattttc
    34381 atgtaaatat cttctccatc taataatttc cccttctatt gactgcgctt ctttaaaaaa
    34441 atttttcata ttccttcctc ctaatcagat agtcagcaaa atctcacttt cttttagtaa
    34501 tgaaatgatg ctgatataaa aattataatt ttatatatta aatttcatgt ctttttaagg
    34561 agttggaata tcatctggaa taggacattt atatgtttta ccattcatag atttatcaaa
    34621 ttcttctttt gcctttttag ctatctctgg attatttaat attttagctc caaacatagc
    34681 cataatcttt gatgcatata tcataccttt ttctcctata gaacttcctg cacaagatgt
    34741 aaactgccaa ctatgacctg gtgctcctag attcgtacac cctgtaaaaa agtatgctgt
    34801 cggaactata tgcattacat ctccaatatc agtagaacca taactattaa agtttgtaac
    34861 atttcctcca ccataatata gatgagttcc ttctggcaaa ttatatttac ttatcatagc
    34921 cttatattgt tgtgggcttt gtctatctaa ttcagctgca aaatccaatt cctctcgagt
    34981 ccaaggctct tgtttaatct cattcataca ttctgataca agatttgcta aaacatgatt
    35041 gtttaaagta ttataacaac ctcctaaaaa ctctacttca acttcagttt cagtcatcat
    35101 tgcagctcct tttgctacct ttacaagtct ttcatatgta ctttctacag cttctctact
    35161 taaagctctt gtataatacc atactgatgc tttatcagga actatgttgg gtgctacgcc
    35221 tccatctgta attgtataat gaatccttac atcagaagta acatgttctc tcaaataatt
    35281 agctcccaca ttagtaagtt ccacagcatc tagtgcactt cttccatttt gagggtctga
    35341 gcctgcatgc gcagtctttc ccttaaagtg aaactttaca gagttaacag ctgttgaagt
    35401 accaattgta gcttcactaa ttttatttgg atgaaaattt atagctaagt ctaacccttc
    35461 aaacgctcca cctctagcca taaatccttt acctgttaat tgttcttctc ctggacatgc
    35521 ataaaatact atagtcccac ttaagttatt ttctatcatc tctcttttaa gccctactac
    35581 tgcccctaca tgtgctgtac ataataaatt atgcccacat ccatgaccat atgcacccaa
    35641 ttcaaatgct tcttgctttg ttgaaacttt ttgatttaaa ccaggtaatg catcaaattc
    35701 tcccataaaa cctataactg gtttacctga accaaaacta gctctaatag ccgtggctaa
    35761 acctcctaca cctacttcca catcaaatcc ttcacttctt agaaagtttg ctgtattttc
    35821 acatgcttta aattctttaa atgctttttc tggattttcc catattttct tacttaaatc
    35881 atcaagtttg aatctttttt tatctatctc atttatagat atacttccta tcattttcta
    35941 ttcctcctat aaatctattt actatcaacc aaatataatt ggacctatta tactagcaaa
    36001 tgccactgat gcaatggtta cagtaacaaa tcctcctata atcatctttg gtaataaata
    36061 atctgttgcc ctttgttttt cttcttcagt tccttccata gcatcaatac cttcattgga
    36121 aattatctgt gttgcaggat aagctagcat acaagttaca ccaattgcag ctgacattct
    36181 tggagaatat cccaatagtt ttcctacaat tgcagtaaat aatattataa atacagaacc
    36241 tacaacaagt attcctatag ttggaacaat catcccaaac aatgctgttg gagttacctt
    36301 agcaagtcct ccaggtaaca tcaacataca accaaacatt ataattccat tagattgaga
    36361 tttatctaaa attccttttt ctaaaaaacc tattcgtgta aaaatcaacc ctactaacaa
    36421 acatgctatg ttgggattta aataatagtt tgcaggggta aatcctggta ttaaagtagc
    36481 caatcctaca aaattaccaa tcatagcaac aaatgaaagc tttgcaagat acactaaagt
    36541 agatgatttt ttacctaaat cttctttaaa aattctcata cttggaagtt taaaattact
    36601 ttttgactca tctttcaaaa aatccccatt atctctttta aattttaatt ctttatttat
    36661 acaatatgta gttatcggaa taccaaagaa cttttgaaat gataatacta gaattacaaa
    36721 tgcagcaagt tcaggtctat ttgcagcttc tgcaacagtt gttacgataa tcccagcaac
    36781 agtagaacct gctattggag gtgctgctat taaagcatac tctctgccaa ataacatact
    36841 acccactgtt aaacagccaa cacctattcc tacaatacct actaatgcaa ttacaaccgt
    36901 tttccattca tgacataagt cttcaagatt tataagtgtt cctaagttag ttattaataa
    36961 agctatacca aagttgctca ttgcagctaa tatccctgat gctcctgtaa tgtcttttgg
    37021 taaaatattt gtccaaaatc ctagtaaaaa tataatacat acaaataaca atgttgaaat
    37081 tgcacctttt gttttcttag ataaaaactc actaaaagca taaatcatta cacatacagt
    37141 aaagcaagta agtggattcc aaaaattttc cattgttgtt tcctcctaaa tataataatt
    37201 ttttatcaat aaaacaataa ttataaaatg tatattatgc agttattctt aactacatat
    37261 ggtatagctt gatatttttt gatgatatta tatacgtcct aaattattcc taaaatatct
    37321 aaataaatat aactagagtt aactctctaa atattaacta tagttatcaa cttactagat
    37381 ttctttttga tttttacttt ctacataatt ttacgagtta gaattgtact tcaactactc
    37441 tctatattag tatacatgtt ggaagactta tatttattta aatattagat atagaataca
    37501 tttttataaa cgtcttaaat tattcttaaa atatctaaaa tgaaataata atatacccat
    37561 taaaccaata ttatcagaat atacttcact ttaagtcttc tttatataga aattttatct
    37621 attagtgcat cccctccttt gtaaattaga ctatttctaa acgaatttag atatcttatt
    37681 cctaggaact attactaaaa acaaaaatta tttattatat taaatcaatg ataagccata
    37741 ctttaaaatt ct
//
LOCUS       DAGSHT010000016        36248 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGSHT010000016 DAGSHT010000000
VERSION     DAGSHT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.017"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..455
                     /locus_tag="MJB72_000783"
     CDS             <1..455
                     /locus_tag="MJB72_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8261908.1"
                     /translation="FEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKN
                     NDICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKR
                     IQTSKDDDNIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            467..607
                     /locus_tag="MJB72_000784"
     CDS             467..607
                     /locus_tag="MJB72_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBY8261909.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            666..1247
                     /locus_tag="MJB72_000785"
     CDS             666..1247
                     /locus_tag="MJB72_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBY8261910.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFVVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            1504..1878
                     /locus_tag="MJB72_000786"
     CDS             1504..1878
                     /locus_tag="MJB72_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261911.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            1961..3670
                     /locus_tag="MJB72_000787"
     CDS             1961..3670
                     /locus_tag="MJB72_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261912.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            3685..4557
                     /locus_tag="MJB72_000788"
     CDS             3685..4557
                     /locus_tag="MJB72_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8261913.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            4558..5346
                     /locus_tag="MJB72_000789"
     CDS             4558..5346
                     /locus_tag="MJB72_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261914.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(5442..6275)
                     /locus_tag="MJB72_000790"
     CDS             complement(5442..6275)
                     /locus_tag="MJB72_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBY8261915.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIKMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(6343..6972)
                     /locus_tag="MJB72_000791"
     CDS             complement(6343..6972)
                     /locus_tag="MJB72_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBY8261916.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            7283..7942
                     /gene="cprR"
                     /locus_tag="MJB72_000792"
     CDS             7283..7942
                     /gene="cprR"
                     /locus_tag="MJB72_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBY8261917.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            8153..9211
                     /locus_tag="MJB72_000793"
     CDS             8153..9211
                     /locus_tag="MJB72_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY8261918.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            9462..11606
                     /gene="fdhF"
                     /locus_tag="MJB72_000794"
     CDS             9462..11606
                     /gene="fdhF"
                     /locus_tag="MJB72_000794"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:9876..9878,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBY8261919.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            11655..12488
                     /gene="fdhD"
                     /locus_tag="MJB72_000795"
     CDS             11655..12488
                     /gene="fdhD"
                     /locus_tag="MJB72_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBY8261920.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            12499..12672
                     /locus_tag="MJB72_000796"
     CDS             12499..12672
                     /locus_tag="MJB72_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261921.1"
                     /translation="MCTKKEKQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            12730..13281
                     /locus_tag="MJB72_000797"
     CDS             12730..13281
                     /locus_tag="MJB72_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY8261922.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            13306..14682
                     /locus_tag="MJB72_000798"
     CDS             13306..14682
                     /locus_tag="MJB72_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBY8261923.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            14682..15197
                     /locus_tag="MJB72_000799"
     CDS             14682..15197
                     /locus_tag="MJB72_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY8261924.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(15275..16558)
                     /locus_tag="MJB72_000800"
     CDS             complement(15275..16558)
                     /locus_tag="MJB72_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBY8261925.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKNLNKERLDKLMQ"
     gene            16746..17168
                     /locus_tag="MJB72_000801"
     CDS             16746..17168
                     /locus_tag="MJB72_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBY8261926.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            17270..18208
                     /locus_tag="MJB72_000802"
     CDS             17270..18208
                     /locus_tag="MJB72_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBY8261927.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            18393..20417
                     /gene="ligA"
                     /locus_tag="MJB72_000803"
     CDS             18393..20417
                     /gene="ligA"
                     /locus_tag="MJB72_000803"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBY8261928.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDRPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            20577..21926
                     /gene="rph"
                     /locus_tag="MJB72_000804"
     CDS             20577..21926
                     /gene="rph"
                     /locus_tag="MJB72_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBY8261929.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNVHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            21926..22396
                     /locus_tag="MJB72_000805"
     CDS             21926..22396
                     /locus_tag="MJB72_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8261930.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            22590..23876
                     /locus_tag="MJB72_000806"
     CDS             22590..23876
                     /locus_tag="MJB72_000806"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBY8261931.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            24026..24610
                     /gene="clpP"
                     /locus_tag="MJB72_000807"
     CDS             24026..24610
                     /gene="clpP"
                     /locus_tag="MJB72_000807"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBY8261932.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            24629..25879
                     /gene="clpX"
                     /locus_tag="MJB72_000808"
     CDS             24629..25879
                     /gene="clpX"
                     /locus_tag="MJB72_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBY8261933.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(25950..26477)
                     /locus_tag="MJB72_000809"
     CDS             complement(25950..26477)
                     /locus_tag="MJB72_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY8261934.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(26465..26977)
                     /locus_tag="MJB72_000810"
     CDS             complement(26465..26977)
                     /locus_tag="MJB72_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY8261935.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            27157..29520
                     /gene="lon"
                     /locus_tag="MJB72_000811"
     CDS             27157..29520
                     /gene="lon"
                     /locus_tag="MJB72_000811"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBY8261936.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITIALAVISAITKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            29510..30121
                     /locus_tag="MJB72_000812"
     CDS             29510..30121
                     /locus_tag="MJB72_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBY8261937.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(30386..31756)
                     /locus_tag="MJB72_000813"
     CDS             complement(30386..31756)
                     /locus_tag="MJB72_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8261938.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(31931..32046)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            32334..33413
                     /locus_tag="MJB72_000814"
     CDS             32334..33413
                     /locus_tag="MJB72_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8261939.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            33804..34484
                     /locus_tag="MJB72_000815"
     CDS             33804..34484
                     /locus_tag="MJB72_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY8261940.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            34477..35856
                     /locus_tag="MJB72_000816"
     CDS             34477..35856
                     /locus_tag="MJB72_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBY8261941.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIKDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            36027..>36248
                     /locus_tag="MJB72_000817"
     CDS             36027..>36248
                     /locus_tag="MJB72_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBY8261942.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNS"
BASE COUNT        14718 a         3900 c         6345 g        11285 t
ORIGIN      
        1 aatttgaaca ttttaaaaat actggaaata aaacattaga tttaatttta gcagaaaaaa
       61 taagtatttg taaaaagtat gatattcaaa ttgaagataa cataaatata tctaaattaa
      121 actttattaa aaataatgat atatgttcaa tatttgcaaa ttcactagat aatgcaattg
      181 aagcatgtat ggatataaat aatgagatag aaaaaagaat agaagttaaa gctacatata
      241 taaataaatt tgcaataata aaatttataa atactaaggt aaatgatatt aaacttattg
      301 ataaacgtat tcaaactagt aaggatgatg ataatataca tggaatagga cttgctagta
      361 ttaaatatat agtaaataaa tatggtggtg aaacaatagt taattactca gataatgagt
      421 ttatattaaa aataatgata ccaataaaaa gttaggagag tacaaaatga aaaaaatagc
      481 gttaaactta cttaaaaaca tctcagcatt aagttttggg atagctgtat tatcagcaaa
      541 cagtgcaagt tcttgggtag cacatcaggc aaaagagcca caagctttac aaaaactaaa
      601 aaaataacat taataaaagg acctcttctt attaaaggag aggttttaca tgttgaggag
      661 gaaatatgtt taaaagatta tcatataaat ttgccaatat attagttaat aatgagattg
      721 tagaaagtga agattttgaa atatatagat atggatttga aactttgatt tatttcatag
      781 ttaatatttt ggtagcatta tttataggaa ttatttttga tagatttata catacagtta
      841 tatttttaag ttgttattgc actttaagac agttcacagg aggttatcat gctaggaatt
      901 ataaagagtg tacattaaca tttgtagtta tttatttgat aactattttt tcagctaata
      961 atatagatat aaataaatat aagtatttat tagtattatt aatgattatt agtatactaa
     1021 caatttacaa gttagctcct ttagaacata gaaacaaacc tcttagtgaa agtgagaaaa
     1081 agcattatag aaagactgtg caaaaaatac tattcgtaat aatatgttta attattttat
     1141 gtaaaatact taatattttt cagcaatatg tgatttatgc aataatttct atatattgga
     1201 ttgctatatt aatatatatt ggaatgaagg ttaatagtga tcagtaagat tataaaacat
     1261 tttctaaatt aattaacttc actaattaat attaacttta aaaaaataat gtcaaggtaa
     1321 gtatttgaac ttattaaata tagttgaatc tattgtgggt tgtatcaaag gacaaataga
     1381 ttcacttaat aaccagtttg taacagagta ttgaattgta aaagttaata cgatagtatg
     1441 aaaatgtaat ttgaaattac atttttataa aaattatatt aactaagggg agggatacat
     1501 acaatgaatt tgaagaaaaa aattaaaaag ttaggagtta catttgcaat aggaactata
     1561 attgtatcaa gtttagggac atctgtattt gcagatgagg ttagcacata tgctttaata
     1621 tctaaagctt tttcaggtaa aaacaatatt atatctagtt cagtatacac ttctggaagt
     1681 agaaccttta aaacgacaga ttctcaaggg ggattttctt gtaggctaac agaaagattc
     1741 tcaggatcta gcccagatag aattgtaaaa agacatgaag caaatgcttc ttcaaaagtt
     1801 agtttatcag gaggaacata ctatataagg tatgaaggtt caacgaataa tgctaaaaca
     1861 tatgcaactt taaattagta ttaaaagcat cctataatta tttttatagg atgtttttgt
     1921 aattgttata atcaaaagac tggctaggag gggcataaat atgagtagta ttaaggttat
     1981 atcagacttt ataaccaaaa taattagaaa aaatatttta tttgcaataa caattattat
     2041 tataaattac catctcatac aacgatggac acatatatcc aatagaggaa tttcatttaa
     2101 tgattactta tccttgagtg tatatacttg tatgacaatt agtattttaa ccatgttaaa
     2161 tgtaattttt tatatgagaa aaagttatga tactgtaaaa atattatgta gaaataattt
     2221 tgattatttt ttaggtattt ctgttgctgt aactagaaac aactttatat tttgttgcat
     2281 accaattata tatgctatag catactcaag tttaataaga acattgtcaa ttgtaacgat
     2341 taaatcaact atatttatat tgatatttga atggttttta ccacttacta ttttatctat
     2401 agtgatatca tttatttcag ttattataaa taatttaata ctaaagatta ccttatcatc
     2461 agtattttta tatttaacat caactaatat gttggataga gttattgata ttaaagaacc
     2521 attttttaaa gcattattta aatcacttaa catatttgaa gatcaatcat atgcttacta
     2581 ttctgatttt gtagggaaag ttaataattc agcatatgtt atagataaga ttatacctac
     2641 actatttgca attactttaa taatagtagc atatctgata ttaaatgata ttagaaagaa
     2701 acagattaaa tcattaatag ttatagtagt gtacagtatt ttatttattg gattaaatta
     2761 tatatcaata gatatgtcaa catatagaga tgaggagcgt agttatggag agtatttaaa
     2821 gaaatctaat attagtgata ttaaaataaa atcgcatgat atgaagattg actttaaaag
     2881 caagtcaaat ataaaagata gaattgtttt attaaacaca tcaaatgaag agttaacaaa
     2941 tattaatatg attttagatg aaatatttga tataaaatat ataaaaataa attcggaaaa
     3001 tataaaattt aacaaaaaaa aggatttaat tgaagttaat ttaaaggaaa gtttagagcc
     3061 taatgacgag gtgacattag atatagagta tgaaggctat attaatgtgt tgacatcttg
     3121 gaatcaaaat aaatatatag ctactaaaga tgatattatg cttccaccag gttctttagc
     3181 gtggtaccca aaagtaaatc aatcaagttg tatggcttat aaaataaatt taagttctaa
     3241 aaataatgtc tattcaaatt tacctttact gaataagact agtaattttt taaaaaaaga
     3301 ttatatattt gaaggaaagt caaatgatat tgcaatatat gcaggtgagt ttaaagaaga
     3361 ggtaaaagat gatataaaaa taatctatcc atcagatagt aatgtaaata tggatataga
     3421 ggaaaaaatt aaatttttaa ttgaaacaag aaatgaaata ttaggtgata gcaaagcatc
     3481 tgtagatgct aaaaaatatg tagatgaagt agataatgtt atggaatcaa taaaaagaaa
     3541 aaaaattaat atgatattag ttgctgactt acctagcaat gaatttttat atgaagactc
     3601 aaagggagag aaaattagca taactattta tcattggctt tcaggaaata tccttataat
     3661 agataaataa taaaggaggt taatatgata aacaagttag aaataagaaa tctaaatttt
     3721 aattacaaaa ataaaaaagc tctggataat ataaatttaa ccctaacaga tggagtagtt
     3781 gcattattag gtcctaatgg tgctggaaaa tcaacattaa tgaggttatt agtgactctt
     3841 tatgaaactt caattggaga gatagaatta aataatataa agtatagcaa aaacaatgag
     3901 aaaatcaagg caaatgtagg atatgtacct caagattttg atatgtataa taatataaat
     3961 gggcaagaat acttagaatt tgtagctaaa atgagaggtg tatctaagaa tgatttaaag
     4021 aaacatatac aaaaagtagt atcaaaagtt aatctggaca agtttataaa taaaaaaata
     4081 ggtacatact caggtggtgt aaagagacga ctaggtatag cacaggcttt aataggtgat
     4141 tctaagttaa tagttatgga cgaacctaca gttggtcttg atccagagca aaggaatgaa
     4201 tttagaagat tgttaccaga aataagtaaa aattctatag tattaatttc aactcacata
     4261 gtagaagaca tacaatttaa ctgtgacaaa ttaattatat taaatcaagg caaaatatta
     4321 tatgatggta caattaataa gtttattgat atggttgagg tgtattcggt agtagtttct
     4381 aatgatgaat ttgaatattt agaacgtaat attgatatta ttgattatag gagagttaaa
     4441 gatggtgtgc aagtaaagta tataggtaaa aatacagaga aacttaaaga tagcttaagg
     4501 atagatacaa ctttacaaga tgcttatata aactttttac ataaggagtt aaaataaatg
     4561 tgtatattgg agttaataaa atttgagctt aaaaaaatag agcttaaaca tgtgttaata
     4621 tttgtaatta tttctatatt atgcatattt attacaggca tattagatta taatgagtta
     4681 atattttata atgatgtacc tataatggat acatattcta tagcaggatg gttaggaatt
     4741 ataaagatat cagatgtttt tataccaata atgtcagcaa tcacaataat ttatgtgttt
     4801 tcaaaagatt attcgggtaa tataaatgag atacttactt tatataataa aaaaaaatat
     4861 aatcaattta tagtatttag atggtgtgtt atattagcta tatatactgt attattatta
     4921 atttcaatta caatagtata tacatttacg tcagtgaaag gttttaacat agacaaaata
     4981 gattttgttt atagtgtaaa gccaatagat gtgtttttaa agacgtttcc tacattatta
     5041 tggtatacaa catttccact aatgatatta acattaacaa aaaatagatt tacaacaata
     5101 gcaatattaa ttacatatac atttgctgat atatttttta ttttacatat gtacccattt
     5161 gttagtatga ttaatgtaaa tggattttat attcaaaaga tgttttatat gcctaacaat
     5221 atagatgtta gatttaatga attaaagaat tatttcttta taaatagagg ttctctagta
     5281 gttatttcta taataatagt tttttatata gcaagaaaat caacggtttt aaaaaagagt
     5341 aaatagttta atatattttt tatattgtta tatttatata gaatagtgac tttaaaaaat
     5401 ttaaagccac tattttttaa tatgttttat ctatataact attatttttc taggtttttt
     5461 aatatttcgt aatagtgtgt tgtggctatt tcacttgact cgcctttttt acaaaaagat
     5521 aatttagata gttttggaac tacacgttca acgacaattt tattacctat cattttagat
     5581 tcaagtatct tcaatgcttc taaaaatcta ttatcctttt ttgcacgatt ataaaatgat
     5641 aaaacgtaga cataaaggaa taggttataa ttacggaaag gatattcaac ttgcataaat
     5701 aatgtgccaa ttccatagtg acaagggccg attggttttt taatagtcca atggtcaagt
     5761 agaaaatcta cagctttgtc aagttttgat tctttattta gataattact aaaacggaat
     5821 gcatttaata tattaagagt tggtagtgga tttgagtatt cagtttctgg acctcgacca
     5881 aaactaaatt tattgcaacg ccacccacca tcagtatact gtgtatctaa aaaatgttga
     5941 aacgtttttt gcattttaat gtcagatgca tatcccatat gacagagtac gttagcagca
     6001 tttgcagtat gacaagggta aatacctcca tttggatata gtttgaaacg tccatcttct
     6061 cgccatgtgc taaatatcag gtcagcacat tctttaagta aagtatcatc agggttcaca
     6121 cctaattcta atagataaag tacactatca agagtagaga aaggagaacc ttttattaaa
     6181 cgcttatcag gtgtagtcca atagtctgca ccattatcat acctgtgtga taatattatt
     6241 tcaatatctg ataaagattg attttctaca ttcatttttt actccatttc atattatatt
     6301 ctattaaatc tatcaaaaac tttattataa tattatttat atttataatt gatttatttt
     6361 atttaaaatt gcatttatag ctgtatcagg aacaactgct aaatcttctg cattgaagta
     6421 ataaactata ttagaatcaa ataatatatt tgaatttgga ggaaattcat catctccaaa
     6481 ccaaagtata aataccatag atgtattacc catatatgta aacctataag ctaaatctcc
     6541 catgtttact ttttcagctc caattttctc cataactaac ttatatttgt ctatttgaga
     6601 tgtaaaaact tctgaaagcc tatatagagt cctgtttaaa aaatttggat aataaacatt
     6661 gcctcctggt atttctttaa aagttacata tttattagtt ctagggatac ctttagcatt
     6721 gattaaatat ctaactatca agattttaag ctcaaagtta ttgcaaaggc taccattact
     6781 attccaaact tctccacttg gatagtctat aatatattgt ctatcaaaaa aattgagtgt
     6841 gaatctattt aaatcattat tgaaaggtat attacttaaa ttactaattg tatgagggtc
     6901 taattctttg agtttttttc gaacatagtc aaatggaaca ttataattat tttttgttat
     6961 cttttcatcc ataatttttt cctccaattt aaacaattaa aagtatattt ataaaaagtt
     7021 ttcttattat taattttatt atatattcaa aatattacaa taataaattg ttaaataaat
     7081 ttttatattt aagtaaatac gtgtaaaatg aatataaagt ttttgaaata taaagttttt
     7141 gtatctaaaa tattttattt gtaaaatagt aattaattat tgtaagctat aatatgagag
     7201 tattatggtt aatgtgaatt ttaaagatat tattaaatat ataaattaga atgctattag
     7261 atggataaat ggaggagtta ttatgtctaa gatattagta gtagatgatg agttagatat
     7321 gttaaagttg attgaaaatg tgttgaaaag agatggacat gaagtaaagg taattagtga
     7381 tgtagaaaag gctatgaaca tggattttaa ttattttaat ctaataatat tagatgttat
     7441 gatgcctaag atggatggtt ttgaagtatg taaaactata agaaataagg ttgattgtcc
     7501 aatactgttt ttgacagcaa aaaatatgga aagtgatatt atgtacggtc ttggtatagg
     7561 tgcagatgac tatataacca aaccttttgg tgtaggagaa ttaagggcta ggataactgc
     7621 tcatttaagg cgagaaagta gagaaaaaaa gaatttatta actatctcaa atgttcaatt
     7681 taacttttta ggcaaagaag tttcaattga tggtaaaaaa attaatttta ctaaaagtga
     7741 atatctaata tgtgaatttc tagcaaaaaa taagggacaa gtattttcaa aagaaagaat
     7801 atatgagtcg gtatatggtt ttgatggaga aagtgatata agtactatca cagagcatat
     7861 aaaaaatata cgaagcaaat taaaacaata tgatatttat acaatagaaa ctgtatgggg
     7921 gatagggtat aaatgggtgt aataagtata agtgttgttt agtgaaaata ttatatatat
     7981 ctacaaaatg tttgtaaaat attaatctat atattaaaat ttaatttata gattaatatt
     8041 ttttgtaaat gacaaaattg taaggtattt tgttataaaa tattaagatt catagtgtat
     8101 actaataata tagtatttag tataaatctt gagcaaagga tggttatttc taatggatag
     8161 actaaagaat attagaaaga gtttactaca gtatacattt ttatttttgt taataggtat
     8221 aacaatttac ctagtgttta aaacattgga tattaaaatg ctatcaaatg ttatggttat
     8281 ggtagataag aagtttttat tcataggtgc tttggcaata atgtgtcaca taatgcttga
     8341 aggagtagta atgaagataa ttatagaaag cactcacaaa gttaatatta gatttattgg
     8401 ttttaagcta gccattatgg gattttatta taatttaata actccttttg cttcaggaag
     8461 ccaaccagta cagatatatg tacttaaaaa atgtaaaatg ccacttagta aggcaagtgc
     8521 agttgttacc aataagtcta ttatcttcca aatagtagtt actttttatt gtacaatact
     8581 tgttttaatg aattttgcta tgttagaaaa acaaatgaaa atgattatgc ctctagtgtt
     8641 tctagggata gccattaatt catttgtctt aattatgata atattagtta ttttaaatcc
     8701 taaaaaaata aaatatttta tgagaattac tataaagcat ttatcaaaaa ttaagttttt
     8761 aaaattttta gataataaga ttgaatccat agaaagtttt atagatgatt atagtaaagc
     8821 aataaatttt tttgcaaaga ataaaaaggt attaatttca actataatag taacttttat
     8881 tcaattaagt gcttacttta gtgtttcatt ttggatatat aaagcattta atttacaagg
     8941 ttatagctat atttatatac tgacattaca agcattttta tatatggcaa tatctccaat
     9001 accaacacct gggaatgtag gagctaatga attagccttt ttcactatat tcaaatcagt
     9061 attccctcaa cctttgatgg gatatgcagt atttttatat ggtggattta tgtactattt
     9121 gattctgatt ggaagtggaa tatttactgt aattactcac tatagaatga aaaatagaat
     9181 tggcagaaat attgttttaa gtgaaaacta aataagttta aagaatatat attgagtata
     9241 ttaaaaaata tgtcataaga actagaatat tttaattaaa tggttgaaat ataaagatta
     9301 ttatgacata ttttttttga aatttaagag gaattgtgag aaaattgttg aatttaatag
     9361 atagtattgc aaaatattta tgattttgca tattataaaa acactcatta tctttaagat
     9421 aaattttact tatcagggga taaatctaag gggggaattg tatggagaaa aaaattttaa
     9481 cagtttgtcc atactgtgga gctggatgcc aattatatct agtagtaaaa gataataaga
     9541 tattaagagc tgaaccggct aatggtagaa caaatcaaga aagtttgtgt ttaaaaggtc
     9601 gttatggatg ggattttcta aatgacccta aaattctaac acctaggcta aaaaagccaa
     9661 tgataagaaa acaaggtgta ctagaagagg ttgaatggga tgaggctata aactatacag
     9721 cttcaagact aaatgaaatt aaagaaaaat atggacctga ttctattatg ggaacaggtt
     9781 ctgcaagagg acctggtaat gaagcaaact atataatgca aaaatttatg agagctacaa
     9841 taggaactaa taatgttgac cattgtgctc gtgtctgaca tgcaccatct gtagccggtc
     9901 tggcttactc attaggtagt ggtgcaatgt ctaattctat accagagata gaaaatgcag
     9961 atgttttatt tatatttgga tataatggag cagattcaca tccaatagtg gcaaatagaa
    10021 tagtaaaagc taaaaaaaat ggtgctaaat taattgttac tgaccctaga gtaactgaat
    10081 ctgcaagaat tgctgatata catctaccta taaaaggtgg aacaaacatg gttttagtaa
    10141 atgcttttgg aaatgtatta atagaagaag gtctttacaa taaagaattt gttcaaaatc
    10201 atacacaagg atttgatgag tataaagaaa ttgttaaacc atatacagct aaatatgctg
    10261 aaaagattac aggaatccca gaagagctta taagaaaagc tatgagagaa tatgcaaaag
    10321 gtaaaaaagc tatgatactt tatggaatgg gtgtctgtca atttggtcaa gctgttgatg
    10381 tagttaaggg acttgcatca attgctctac ttacaggtaa ttttggtaga gaaagcgttg
    10441 gaataggacc tgtacgtgga caaaataatg ttcaaggagc ttgtgatatg ggagctcttc
    10501 ctaatgtata tcctggctat caaaatgtaa ctgatgataa gataagagaa aagtttgaaa
    10561 aagcttgggg ggtaaaatta tctcctaata atggttatag tttaactcaa gttccagact
    10621 tagtactaaa agagaaaaag cttaaagctt attatatatt tggagaagac ccagtgcaaa
    10681 gtgacccaga tgcgtcagaa gttagagaag cattagatga attggaattt gtcattgttc
    10741 aagatatatt tatgaataag acagcacttc atgctgatgt tatacttcca gctacttctt
    10801 ggggagaaca tgagggagta tatacatgtg cagatagagg attccaattg atgagaaaag
    10861 ctatagagcc acaaggagat gtaaaaccag actggcaaat tataagcgaa atttctacag
    10921 caatgggata tcctatgaat tataaaaata ctaaggaaat atgggacgaa cttagacaat
    10981 tatgtccaag tttcttaggt gctacttatg aaaagataga aactcaagga tgcgttcaat
    11041 ggccttgtaa gagtgaaagt atggaagata aaggaactat gtatctgtat gaaggtcaaa
    11101 agttttctac tcctaatggt aaaggtaatt tgttcgctgc tgaatggaga ccccctatgg
    11161 aagttgaaga tgatgaatac ccatttagtt tatgtactgt aagagaagtt ggacactatt
    11221 cagtaagaac aatgactggt aattgtagaa cacttagttc attagaagat gaaccaggac
    11281 gtgttcaaat aaattctaat gatgctgaaa aattaggaat tgaagatgat gaattagtta
    11341 gaattagttc aagaagagga agtgtaataa caagagcaac agttactgat agagttaagg
    11401 aaggtgcaac atatatgact tatcagtggt gggttggagc ttgcaatgaa cttacaatag
    11461 caaatttaga ccctatatct aaaacaccag agtataaata ttgtgctgtt aaacttgaaa
    11521 aacttgaaga ccaagaatta gcagaaaaat gtgtaagaga agagtatcaa agtttgaaag
    11581 acaaaatgac agctacaaat atctaatgat ttatttgatt taagatttat aaaaggcaga
    11641 aaggctaata ttttatgaac aatatatact caaaaactaa attacaagaa aatagtaagg
    11701 ataaagtttt tgaaaatagt tatagtctag tggactacaa atatttaaat tcttttaatg
    11761 tagaagtaag caaaataaat gaacatgaag tatcaataga taatgaagaa ataattgcag
    11821 aatatccact tagttttatt ttaaatgata aatatgtaaa tacattttta tgtaccccat
    11881 ataatttaga ggaacttata gtaggattct tgaaaacaaa aggatacata gaaaacaaaa
    11941 aaagcttatt aaaatttgag ataaatgaga gtgaacgttt tgcaaaagta actattcaag
    12001 agaaacaaaa aaatcaagat gagcaaatta tatttttaaa ttcactagac tatataacct
    12061 gtgctcctgt aaagagcaat ataaaaataa attatgatac gatttacagt ataatggata
    12121 aaaatttaaa ttcatcagaa ttatttaaaa atactggtgg ggtacatagc gtatctgtat
    12181 ttaattttga taaaaaaata gtcacttgtg aagatgtagc aaggcataat gcaatggata
    12241 aggctattgg attttgtgtt ttaaatgaca taacattaaa agataaaatt gtggctgtaa
    12301 gtggaagaat atcattggaa atgatattaa aagcagctat gactcaaatt cctattgtag
    12361 tatcaaaatc agcaccgact aatttatcaa tagagctttc taaaaaatta aatataacgc
    12421 ttattggatt tgtaagagga agacggatga atatttatgc aaatccacag agagttatca
    12481 aaaagtgaga atgaatatat gtgtactaaa aaagaaaagc aaagatattc aaagtgtgaa
    12541 atctgtggag tattaattga tgatactgat aaggtgtata ataacacata caatttgtgc
    12601 aaaaaatgta aacgaaatat aagtactgag aaaatagtga aatatataaa tatatatgat
    12661 gaaagtaagt agacatatga tgaaaataac tagatggttg gaaatataca aattgaaagg
    12721 tgggataata tgaaaaataa aataggctct tttgtaatag cagacccaaa caaatgtaca
    12781 ggttgtagag cttgtgaagt agcttgtttt acaatgcata atcagcataa taatgtgggt
    12841 tatacagttg gaacagttga tatacctgtt atacctagac tttacttagt aaaaggtgac
    12901 aatttctgta tgccaataca gtgtagacat tgtgaagatg ctccttgttt aaactcatgt
    12961 ccacaaaaag ctatagttaa agaaaacaat attatgtctg taaatgaaga aaagtgtata
    13021 ggatgtaaaa cttgtctgct agcatgtcct tttggagcta tagacttact tccacaatat
    13081 gaagatggta aagaagtaga acaagtaatt ttagatgaaa ataagaagat agcttataaa
    13141 tgtgacttat gtaaagacaa tgaaaaaata gcttgtataa gtgcatgtcc tcaggaagca
    13201 ttaaaacttg ttactccaat agatgacaaa aaagctaaga atagacaagc tgcacttagt
    13261 ttattgttaa caaataaata aaggatacag gggtaggtga cgtttatgat aataaatata
    13321 gataaggatt tatgtactgg atgtagagaa tgttcaaagg tctgtccagt aaatgctatt
    13381 gaaggtgaag agggaaagcc acaagagata aatttagata gatgcgttat gtgcggtcaa
    13441 tgtgttcaaa cttgtaaatc ttatgcctca gttatagatg aaggatttga gtttttacaa
    13501 gagaaaaaac aagaaagaga gataccagag tctataaatg aacctatatt tgcagcatac
    13561 aatgtatgtg atatagataa ggtgaagaaa gctttaagtg acccaaatgt attcactatg
    13621 gtccaatgtg caccagcagt tagagttgct ttaggagaag attttggata ttatttagga
    13681 tacttaggtg ctgggaaaat ggcagcagca cttagagctt taaattttga tagagtctac
    13741 gatactaatt ttgcagcaga tttaactata atggaagaag gtagtgaatt aattaaaaga
    13801 gtgacagaag gtggaactct tccaatgttt acatcttgtt gtccagcttg ggtaaaattc
    13861 atggaaaaaa attatcctaa attaactaat catttatctt cttgtaaatc acctcagcag
    13921 atgggtggtg caatatttaa aacttatggt gcagagataa ataatgtaga tgcatcaaag
    13981 atatttagtg tttctataat gccatgtaca tgtaaaaaat atgaatgtga tagagaagaa
    14041 atggattcta gtggatatag agatgtagat gtagtattaa ctactagaga actagcttat
    14101 ttaataaaag acatgggaat tgattttaaa aatttaagag aagaaaaatt tgatagtcct
    14161 ttaggtagtt atagtggagc tggaactatc tttggtgtta ctggtggggt tatggaagct
    14221 gctataagaa caggatatga gcttataact ggagaaagta ttcctgatgt agaagtaaaa
    14281 caagtaagag gagaaaatgg ttttagaaag tcaactatta aaataggaga cttagactta
    14341 agagttggag tagtaagtgg attaaaaaat gtaataccag tgcttgaaga cttggaaaaa
    14401 ggaaaattag atgtagattt tattgaagtt atgacttgtc ctgttgggtg tgtaagtggt
    14461 ggaggtcaac caaaaatttt actagaagaa tataaggaat tggcttatga aaatagaaca
    14521 aaagcaacat acgtgcatga tgaacattta gcaattagaa aatctcatga gaatcctgat
    14581 attaaaaaac tgtatgatga atatctggta gaaccattag gagaaaaatc tcatcatcta
    14641 ttacacacta catattgtat aggaaaggaa gtggctaagt gatgaactat tttgttatag
    14701 cagaccctga taaatgcata ggatgtagaa cttgtatgat aggatgtgta gttgcacatt
    14761 cagatgaaga tattttttac caaaatccag atgaaataaa ttttaatcca aaactttctg
    14821 tcataaagac aaaagaagta agtgctccaa tacagtgtag acattgtgaa gatgctcctt
    14881 gtgcaaaggc atgtccaaat ggtggtatag ttagggttgg taacacaata aaaatcaatg
    14941 aagaaaattg tataggatgt aagacttgta tgttagcatg tcctataggt gcaatagaca
    15001 tagttacatt aaaagatgta gatgaaggaa agctttgctt tagagaaaga atgacagcaa
    15061 ataagtgtga tttttgtata gaatcgccag aagggcctgc atgtgtaaat gtttgtccaa
    15121 ctaaggcatt tactatagtt aaggatgagg atatagatag taaagttaaa aataagagaa
    15181 aattagctat attgtaatat aaaaaagatg gagttttatt tatgattaaa agctccatct
    15241 tttttattat aatatttata tatctaaaaa tcaattattg cattagttta tctaatcttt
    15301 ctttattcaa atttttcatt cctatagaag ttataataag taaagtatag gcaattataa
    15361 ctgataaaat tataatagac caagaaaatc tgtatccaaa attatcaact aacaatccaa
    15421 ataatgttga acctaatgca aatcccactg caaaaattaa ttgtacgatt ccatatatgc
    15481 taccaaactc cttttgacca aagaaagagc cagttaaata agcaggacca atcatataag
    15541 caaatacaga aagacctttc aaaatggcaa atacaaatgc aaaagtagaa gatttatcta
    15601 ccaatagtaa tgataaagtt gctataagaa ccaatacaaa agatataata agtggctttg
    15661 tttgaccgaa tttatcaaac aataatcctc caaataaatt accaatgaga gcaaataaag
    15721 caaatgtgga gcctactgtt cctattagaa taggatttag ataatttcta agataagttg
    15781 gatattgtac agataatgat gaaacatata taccaacaaa tataaatcca atagtcataa
    15841 tccaaaaata tttaattttt ttagcttctt ttaatgtata accccatact gaaggagaaa
    15901 ttgtatcaga tgctttctct tttttacctt tagattttac cttttcatta atatctttag
    15961 gcatcctgag tataaataat attacaggta taccaactat taatgctata attccaaata
    16021 ttagataaga acgactgtat ccttgagttg ctaataagcg aactgcaagt ggttgtagaa
    16081 atatactacc aatagaacca ccagcaaaag caaagcccaa agcttttcct ttgctttctt
    16141 catcaaacca agcacttatt aaaacaggta ttcctatagc agatataatt gtagcgccaa
    16201 tttgaacaat acatgataca gtgtaaaatt gccatagttc agtacatata gaaaaagtag
    16261 caaaagcaat actagaaatt atacaaccta ataaatatag tatcttaata ttgactttag
    16321 aaaataatac acctataaaa ggtgatgcaa ttgctgaggc aatagtacca atagtgaata
    16381 ttagagaaaa agaagctaaa ctaaagcctt ctccttctac aacataactt ataaattgag
    16441 gctgtatact tgctacaata ctaaatggaa cagcttgtat caacatacaa ccaagtacta
    16501 taacccaagc atttgaaaag tttttaatgc taaatttatt gtttagtaat gtattcatgg
    16561 tatctctcct ttaggttatg atttgtagat attaatctaa ttattctaat tttatatatt
    16621 agttttggat agcctaacta tttaaatata atttgggtat taatataaaa attaactata
    16681 aaaattgatt attttaaatg tatggggtaa attaaagtat ataaataaat gaaaggatgg
    16741 ataaaatgtc atttgaaaaa ataaataata ataacctaga taaaatacac aacaggaagt
    16801 gtattatgct tgtaaacttt aataaaaaag aattatcatt aattaaaaac gtaagtaact
    16861 ttgctggaat aagagaccat atcatcttaa gtgataaaaa tggagagaat ataataagag
    16921 acattttaga tggaaatata tcaaatgaat gtgaaaatgg gattaagagt aagtctgtaa
    16981 tatttaataa cactccaagc acacaagtaa atgcattttt agaagggcta aaaaaagtga
    17041 gaataaatag accttttaca gctatggtaa cagagacatc tattgattgg acattgaata
    17101 atttaataga aaatttagtt caagaacgta atgctatgtc tttaggaaaa acaattgaac
    17161 ataagtaaat ttaagatatc ttatagtaat tttaactaat gtacattgag tatcatatta
    17221 tattttagaa tataaaattt taaatgtggg tagatggagg agatttgaga tgaaagtttt
    17281 atttacaata aattatggaa aagaaaaatt tgataaaata agagaattag gctatgatgt
    17341 catacattac tctgaagata gtattgaaaa taatgaggat gtaaatacag cagatgtact
    17401 agttacatac aatccatttg agagattaga tatttctaag atgaaaaatt tacaatatat
    17461 acagacaact agtgtaggta tagaccaact tccaaaagaa gaggtattga agagaaatat
    17521 taagatagct aataatagag gtggatatag tgtacctatt ggtgaaagta ttgtattgta
    17581 tattttagaa atatataaaa atagtgcaaa attttttaaa caacaacaaa ataaagaatg
    17641 gaagatggat ttttctgtat cagaattgta tggacaaaga gttggatttt taggaacagg
    17701 tactatatct tcagaagcag caaaaagatt aaaagctttt ggtgtagaaa tatggggtgt
    17761 caatactgat ggaagtagta aaaaatactt tgataagtgt tttgctactg aggatatgaa
    17821 tattgtattt gaagaatgtg atatagtagt tgtagcaatg ccttctacaa aaagtacaga
    17881 tggaatagta aacaaagata tgtttaaact tatgaaagat ggttctgtat ttataaatgt
    17941 aggtagagga aatacagtta atcagaaaga tttagaagaa tgtgtagaga aatttagagg
    18001 ggtagcatta gacgtatttg aaagtgaacc tttaaacaag gacaataagt tgtgggagtg
    18061 cgaaaatgtc atagtaactc ctcataattc ttgggtgtca gataaaaaca aagagagaac
    18121 atttaatatg gtttataata atttgaaaca ctatataatg gagaataaac ctgtagataa
    18181 tgttgtagac atatcaagag gctattaaaa ataaaaaatt ttgataaaaa taaaaactct
    18241 tattagtcgt attaaagata ctaataagag tttttttgta ataaatttaa agccaaaaaa
    18301 gtgatatact aaaaaatata taaataggag ataattaatt atagggaaat ttcaaaataa
    18361 tttaatttta aaaatatgaa gaggtgcaaa aagtgagcgt aaaagatgaa attaataaac
    18421 ttatagagac aataaactac cataatgaaa ggtattataa tcaagatagg ccagaaatat
    18481 cagattatga atacgataag ttaatgaagg agttaatttc tttagaagaa gaacatccag
    18541 aactaaagag aattgattct ccaacaaata gggtaggagg taagccatta gataagttca
    18601 atcaaattgt acataaaact cctatgttga gtttatcaaa tgctttttca gagcaagatt
    18661 tgagagattt tgataagaga gttcaagaat atgttggaga aaatgttgaa tatgttgttg
    18721 aatttaaaat agatggtctt tctgtaggtc taacttataa caatggagag tttgaaaaag
    18781 gagcaactag aggagatgga gttgtaggag aagatatttc tcaaaactta atgacagtaa
    18841 aaagcatacc tcttaagata aatgataaaa aagaactaat agttagagga gaagtatata
    18901 tatcaaaaga taattttaga aaagtaaatg aacaacaaga agaacaagaa cagccattat
    18961 ttgctaatcc aaggaatctt gcagctggtt cattgagaca attagaccca aaactaacag
    19021 caaagagacc attagatata tttgtattta atatggaaaa tatagaagaa tacgatttag
    19081 aaagtcacag tgaatcacta gagtacttag aaaaactagg attttcagta agtcagagtt
    19141 ataaagtttg taaaagtata gatgaagtta ttgagcatat tgaatactgg acagataaca
    19201 ggggtaattt agactacgaa atagatggta tggtaataaa agtaaacaat ataaaacaaa
    19261 gagacgaaat gggatataca gccaaaagtc ctagatgggc aattgcatat aaattcccag
    19321 cagaaaagaa gaaaactaaa atagtagata taatagtaga agttgggaga actggtacaa
    19381 taacaccttc agcaattctt gaaccagtca gacttgcagg tactactgta agtagagcta
    19441 cacttcacaa tgaagattat ataagagaaa aagatataaa aataaatgat acagtattag
    19501 ttcaaaaagc aggggacata atacctcaag ttttagaagt tataaaagaa gaacgtactg
    19561 gagatgaaat agattttaaa atgccagaaa agtgtccagt ttgttcagag ccaacaataa
    19621 gattagaagg agaggctgct gtaaaatgta tcaatatgtc ttgcccagcc caaataagaa
    19681 ggggaattat acactttgtg tcaagggatg caatgaatat agatggattg ggagaatcta
    19741 taattacact gctattaaat gaaaaaatta ttcaagatgt atctgattta tattatataa
    19801 aaaaggaaga tgtagtcgac ctagagagaa tgggcgaaaa atctgctgac aatttaataa
    19861 attcaattga aaaatcaaaa gaaaatgatt tatggagact tataaatgga ttaggtatta
    19921 aatttatagg agttaaagca gctaagatac ttgcatataa ctttaaggat ttagatgaag
    19981 taatttctgc aactagtgag caactagaag aattagagga atttggaagt ataatggcaa
    20041 acagcgttgt cgagtttttt aaggaagaca aaaatatgaa cgttataaac aagttaaaaa
    20101 atgtaggagt aaatacaaaa tcattggata atacaggtga aaaagtagaa aaaatatttg
    20161 aagggatgaa aatcgtattg actggtacac ttcctacatt aaaaagaaat gatgcaaaag
    20221 atatgataga aaaaagaggt ggaaaagcta catctagtgt tagtaaatcg acaacatttg
    20281 ttttggcagg tgaagaagct ggttctaaac ttactaaagc aaatgattta ggaattaaag
    20341 ttatagatga agagagattt ttagaattat taaagttatc atcgaaagat gaagtaaaag
    20401 ctgtattaga gagttaaaaa gtcgcttaat ttagtagaaa actactataa ttaatgatag
    20461 aatagttcag taagctaata agattacgaa aaatgacaat aattaatatt tgacaaaatt
    20521 aaaaattata aaaataaatt tataaaaaat aaaaaatatg agaaaggata taaaaaatga
    20581 ccagaagtta tgatagaagt aatgaacaga taagacctgt aaagataact agagattata
    20641 caaagtatgc agaaggttct gttcttatag aaatgggaga gacaaaggtt atatgcactg
    20701 catcagtgga agaaaaagtt cctccttttt taagaaatag tggtactgga tggataaatg
    20761 ctgaatattc tatgctacca agagcaacac aacaaagaaa gataagagat gcatctaggg
    20821 gaaaaattga tggaagaaca caagaaatac agaggttaat aggaagagca ataaggtctg
    20881 ttgtagactt gaataaatta ggagaaagaa ctatttgggt agattgtgat gtgattcagg
    20941 cagatggtgg aactagaact gcatctataa caggggcatt tgtagcagtt gcagaagcaa
    21001 tatataaatt atataaagca aagacaataa aagagatgcc aataacaaat tttgtgtctg
    21061 caattagtgt tggtattgta aatggaatac acatgctaga tttatgttat gaagaagatt
    21121 ctaaagcaca ggtagatatg aatataataa tgactgataa gtgtgagttt gtagaagtac
    21181 aggggacagg agaagagaaa cctttttcga gagcagattt aaatttattg ttagagttag
    21241 gtgaaaaagg gaataaagaa ttaataaggc ttcaaagaga agctttaggt gaaatatcag
    21301 atgagatatt gggtatggat tatggtaatg aagtagtgat tgctactaat aatgttcata
    21361 agctagaaga aataggtgaa atcttaaaag attttgaata taaagtgtac tctttaaaag
    21421 atgttgattt agcaggtata gagatagtag aagatggaaa aacatttgag cataatgcgt
    21481 tgataaaagc tagaacaata gcaaaaaaga ctaaacttat agcaatatca gatgattctg
    21541 gcttagaagt agatgcttta ggcaaaaaac ctggtgttta ttcagctaga tatgcaggag
    21601 aacatgcaac agatgaagaa aatagaaaaa aactgttaaa agctatgcaa aatgtaccaa
    21661 tgagcaaaag aaatgcaaga ttcgtctctg caatagcagt agtttttcct gatggaaaag
    21721 aatttgtagt aagaggaatt tgtgaaggaa tgataggatt tgaagaaaaa ggtaaaaatg
    21781 gttttggata tgatagctta tttatagtaa aaggatatga taagactttt ggtgaaatac
    21841 ctagtgttat aaaaaattca atcagtcata gagcaaacgc actaaaactt atgaaacaag
    21901 agtttataaa aagggtagtg aagtaatgaa aataggcata gtaagcgaca cacatatgat
    21961 gattaaaaat atggaaaaaa caataccata tttgaaagaa tgtgacctaa taattcatgc
    22021 aggagataac tttactgatt caagatatat tcatagcatg actaatgtag gtattattgc
    22081 agttaaaggt aattgtgact ttgatgctgt tgaggaagaa gttgtctttg aagtggcaaa
    22141 taaaaccata ttcttatgcc atggagataa gtatggtgta aaatatggaa ctaatatgtt
    22201 agaaaaaaag gcaactgaag ttgatgctga tatagttata tttggccata cccatacccc
    22261 atttagagaa attaaagatg gtgtacttta cataaatcct ggaagcactt cacttccaag
    22321 aggagtatcg tataaaagtt ttgtcattat ggatatagaa gaagatgaca taaaaattga
    22381 agaaatacgc atataaaggt gtatttatct aaaaaaatgg caaaaaacgc tcaaaaaatt
    22441 aaattttatc aaataagtac aacgatatta atggaacact tgtaaataaa aaaacttatg
    22501 tgatataatt aagtggttga attataaata gaatgaatta taataccgtt atcgggtatt
    22561 acataaatat cactaggagg attagaagga tgaaagcaga actaattaaa aaagagggta
    22621 ataaagttac tttaaaaata acagtagata acaataaatt tgaagcagca gtaaataagg
    22681 cttataataa aagcagaaat aaatacaata tacctggatt tagaaaaggt aaagctccaa
    22741 gaattgttat agagactcaa tacggaaaag gtatattcta caatgatgct atagaaatat
    22801 tattcccaga agtttatcca gaagctatca aggaattaga catagaccca atagacaatc
    22861 cagatttaga tatagaagaa ataagtaaag acaatgggtt ggttatggtt cttaatgtag
    22921 aagttaaacc agagtttgaa ttaggaaact acaagggtat agaaatagct aaggttgaga
    22981 atactgtaag tgacgaaaat gttgatgcta aactacaaga aatggttgaa aaaaatgcca
    23041 gattagtaag tgtagaagac aaagctttag aagatggaga tacagctata atagacttcg
    23101 aaggttttga aaatggtgta gcttttgatg gtggtaaagg tgaaaattat aacttagtta
    23161 taggttcaaa cacatttata ccaggatttg aagaacaatt agtaggtaaa aaagctggtg
    23221 aagaagtaga agttaatgtt actttcccag aagaatatca ctcacaagat ttagctggaa
    23281 aaccagttgt atttaatgta aaaataaatg atgtaaaagt aaaagaatta tctgcacttg
    23341 atgatgaatt tgctaaagat actagtgaat ttgattcttt agatgaatta aaagcagatg
    23401 taagagctaa gttagaagaa gaagctaaaa atagagcaga tgcagaaact agaaattcag
    23461 tagtagaaaa agttgctgaa aatacagaaa tagaaatacc agaagttatg atacaacatc
    23521 aaatagataa tatgttaaat gaacttaact accaattaca atatcaagga tttggattac
    23581 aacaattact agagatgact ggtagaacta tggaagaatt aagagaagag agaaaagaag
    23641 atgctaaaaa attagttaag tcttctttag tattagaagc aataactaaa gctgaaggaa
    23701 ttgaagcaac agaagaagaa tttaaggctg agttagaaaa aatggcttct gcttacaata
    23761 tggaagtaga aaaaatagaa gcttctttaa gagatgccga caaagaagat ataaaaggtc
    23821 aaataaaaat aagaaaaaca atagatttat tagttgataa tgcaacaata gcttaataaa
    23881 ataaacgcta actaatatag ttgacgttat gaaatgttta gtatataatg atgtgcaaat
    23941 ataatttagt taaatgcgta taataattta cagtagctta aaaaaagcta ctgtaaatta
    24001 tatagaataa agggggtatg aaaccatggc attagtacct gtagttgttg aacaaacagg
    24061 aagaggagaa agatcttatg atattttttc tagactatta aaagacagaa taatcttctt
    24121 aggagatcaa gttaacgatg ccacagcagg gcttatagta gcacagctat tatttttaga
    24181 agctgaagac ccagacaaag atatacattt atatataaat tctccaggag gaagtataac
    24241 ttctggaatg gctatatatg atactatgca atacatcaaa ccagatgtaa gtactatatg
    24301 tataggtatg gctgcttcta tgggagcatt cttgttggca gcaggagcaa aaggaaaaag
    24361 actagcacta ccaaatagtg aaataatgat acatcaacca ttaggtggag ctcaaggtca
    24421 agcaacagat atagaaattc atgcaaagag aattttaaaa ataaaagaaa ctttaaatga
    24481 gatattatct gaaagaactg gtcagccatt agaaaaaatt aaaatggata ctgaacgtga
    24541 taacttcatg agtgcactag aagccaaaga atatggttta atagatgaag tatttacaaa
    24601 gagaccgtag atagagagag gtgttaatat gtctaaatat gaagaaaaac gacaattaaa
    24661 gtgttcattc tgcggtaaaa atcaagatca agtaagaaga ttaattgcag gtccaaatgt
    24721 atatatttgt gacgagtgtg ttgaactttg tgacgaaatt attcaggaag aaatagaaga
    24781 tactatagat gaagatacta catctttacc aaaaccaaag gaaatgatgg agattctaaa
    24841 tgattatgta ataggtcaag aaaaagctaa aaaagcttta tcagttgcgg tttataatca
    24901 ttataagaga atatacagta aaaaatctag tagtaaggat atagaaattc aaaagagtaa
    24961 tatactttta ttaggaccta caggttctgg aaagacatta cttgctcaaa ctctagcaag
    25021 aactctaaat gtaccttttg caatggcaga tgctacatca ttgactgaag ctggatatgt
    25081 tggagaagac gttgaaaata tacttttaaa acttattcaa gctgctgatt ttgatataga
    25141 aaaagctgaa agaggtataa tatatataga tgagatagat aagattgcaa gaaaatctga
    25201 aaacccatct ataactagag atgttagtgg tgaaggtgtt caacaagcac ttctaaaaat
    25261 attagaaggt actgttgcaa atgttccacc acaaggagga agaaaacatc ctcatcaaga
    25321 atttttaaag atagatacta ctaatgtatt atttatatta ggtggagctt ttgatggatt
    25381 agagaagata attcaaaaac gtggtggaga taaaacacta ggttttggag ctaaaataga
    25441 aagtaaaaaa gaattagatt tagggaagct gtatgaaaaa gtacttccag aggatttatt
    25501 aaaatatggt ataataccag agtttatagg aagaatacca gttttagcta ctttagagtt
    25561 gttggatgaa gatgcattaa tgcagatatt acaggaacct aaaaatgctc tagtaaaaca
    25621 gtataaaaag ttattggagt tagatgatgt tgagctagaa tttgaagaag gtgctcttcg
    25681 tgcaatcgct aaaaaggcca tagaaagaaa tacaggagct agaggactta gaagtatagt
    25741 tgaaagcgta atgatggaaa ctatgtttga agtaccatct agagataata taaagaaggt
    25801 tatagtaaca gagaaatctg tgaacgaaga ctcagtaaat cctatcatag tacttaagga
    25861 tcaagaggaa agtgcataag atgatgaaaa ggagctttaa aagctccttt ttttatgtta
    25921 atatgctaaa tataaaatag gtatcattat tataaaaatg tgagtaatat acctagaata
    25981 ccaaagacga atacaattgc aatggaacca atttttcctt tatataaaag ataaaatgat
    26041 gctataaatg caattattcc atataaatcc caattaactt ctttggcaga tgaaaaagct
    26101 gctgataata tgaatcctag agtaattggt ctaagaactg tcaataagtt ttctaccttt
    26161 ttattatttt taaatttaga aaataacttt gatataatag ttagacctat taatgcaaaa
    26221 attgtaacac caatagttgc agctagagaa ccaaagaagc cagcagtctt aaatcctaca
    26281 aatgtagcac tatttattgc tacaggacct ggtgtagatt gtgatatagc aagtaaatcc
    26341 aaaaactctt gattagttat aaagtgatat ttagatataa attcttgctg gataaatggt
    26401 agcatggcat aaccgccacc aaaactaaat gcccctattt taaggaatat taagaagagt
    26461 agtattattt ctgacataga ctatatacac ctcctccaat tattaaataa atagggctta
    26521 tattgaatac accaacagat aaaactgcta taattaatag tagtatgtta gttttagact
    26581 ttggtagttt cttgaacatg ctcgtaaaag aatacaatat aagagctatt acaacaggcg
    26641 caactccttt aaaaaatcca tctaaaattt tagatgatga atttttaaag tatagatacg
    26701 ataagactaa gactatacaa aatgatggta aagcagagcc taaggtacat ataagaactc
    26761 ctggtatacc ataaagttta tatcctaata atacagatat attaactgcc agtactccag
    26821 gataactttg agctatggat aaataatcaa taaattcttc ctgctctaaa agtttttgtt
    26881 tgtttacaag ttcatcttgt ataagaggaa ccatagcgta tccaccacca aatgtaaacg
    26941 caccaatttt taaaaaggtt aaaaacaatt tcaacataat agaaacctcc aaaagataaa
    27001 taacatctaa gtatactaat attaatatta tatcataata aaaagataat atagtatgat
    27061 agtattctaa gaatttatca ttaatgttga aaattaactt gttcaaatat ataatattaa
    27121 aagcaaagca attagactag aaaaggagta atgttgatgg aacaaaatta tactaaaata
    27181 gatcatgaat taccattaat tcctctaaga ggattagcta tatttccata tatgatatta
    27241 aattttgata taggtagaga gatatctctt aaagctttag accaagctat gatggatgaa
    27301 gaacttatat ttttaacttc tcagaaagaa gcagaggtag atgagccagg agaagaggat
    27361 ttttatcatg tgggaacaat ctgtaaagtt aagcaaatga taaaattacc aggagataca
    27421 gttcgtgttt tggttgaagg tgtatcaagg ggtagagtaa agaaaataga acaagaagat
    27481 gggtatttca gagcagtaat agaagaaata gtatttgata gtgataattt ggatagtgaa
    27541 acagaagttg aaattgaagc atttgtaaga aatgtatttg atgcctttga agagtatata
    27601 aatattggaa atagggtatc accagaaata ttaatatctt tagctgatat agaagatgta
    27661 gatagattta tagatacgat agctgcaaac atatatctaa aatcaagcca aaaacaagaa
    27721 atattagaag aatttgatat tagaaaaaga cttgaactta tttattctat attattagag
    27781 gaaatagata tattaaaaat agaaaagaaa ataactttaa gagtaaaaaa acagatgaat
    27841 aaggttcaaa aagaatatta tcttagagaa caattaaaag ctatacagaa agaattaggt
    27901 gaagaagaag atataaactc tgaagcagat gagtatagag aaaagcttaa aaaaataaaa
    27961 gcacctaaga caactaaaga gaaaatagaa aaagagatag ataaattttc aaaaatatct
    28021 tctatgtctc cagatgtatc tgtaagtaga aactatttag atactatatt ctcattgcca
    28081 tggaataaag agacaaaaga taaattagat ataactaagg caaaagatat attagatgaa
    28141 gaccattatg gattagaaaa agttaaggag agaatactag agtatctagc tataagaact
    28201 cttgctaagt cacttaaagg tcctataata tgtctagttg gtcctccagg cacaggaaag
    28261 acatctatag taaaatcaat cgccagagca ttaaatagaa aatttgtaag aatatctttg
    28321 ggtggagtta gagatgaagc agaaataaga ggtcatagaa gaacttatgt aggttcaatt
    28381 ccaggaagaa taattaatgg ggtaaaagaa gcccaaacaa aaaatcctgt attcttattt
    28441 gatgagatag ataagatggc agcagattat aaaggtgacc cagcttcagc aatgctagaa
    28501 gtattagacc cagagcaaaa taaggatttt gtggaccatt acttagaaat accttttgat
    28561 ttgtcaaaaa tactatttgt tacaacagca aatagtctag gtaatatacc aagaccatta
    28621 ttagatagaa tggaagtaat agaagtatct gggtatatag aagaagaaaa attaaatata
    28681 gcaaaaaaat acctacttcc aaaacagatt aaagaacatg ctttaaaaga aagctttata
    28741 aaaatagatg atgaaacttt aagaagtata atcaaccatt atacaagaga agctggagta
    28801 agaacactag aaagaactat tggtaagatt tgtagaaagg ttgccaaaaa atacgttgaa
    28861 gacccaactc ttgaagaggt tgttataaat aagtctgacc tagagacata tcttggaaaa
    28921 gacatgttta agtatcaact tgcagaagtt aaccctcaaa taggattagt aaatggtctt
    28981 gcgtggacag aagttggtgg agttacactt gaagtagaag taaacgtact taaaggtaaa
    29041 ggagaaattg tattaactgg aaaattagga gatgttatga aggaatctgc gaagacaggt
    29101 atctcttata taaggtcaat agtggacaag tttgatatag accctgaatt ttataagacg
    29161 aatgatattc atatacacat tccagaaggt gctgttccaa aagatggacc atcagctggt
    29221 ataactatag ctcttgcagt aatctcagca attactaaaa gacctgttcc tggaaatata
    29281 gcaatgacag gagagataac tttaagaggt agagtcttag cagtaggagg agttaaagaa
    29341 aagttattag cagctcatag agctggtata acaaaagtat taattcctaa agaatgtgaa
    29401 gctgatttag atgaaatacc agaaaatgta aaagaaaaaa tggagtttgt gcttgtagaa
    29461 catatggatg aagtattaga acaagcttta ttaaagagtg gtgaaaataa tgaaaattag
    29521 aagttcagag ataactatga gtgctgtgaa taaatcacag tatccagcag aaggaatccc
    29581 agagatagca ttggctggta ggtcaaatgt tggaaaatca tcaataataa atacattgct
    29641 taacagaaga aactttgcta gaaccagtca aacaccagga aaaactagaa ctataaattt
    29701 ttatctaata aataatgaat tttattttgt agaccttcca ggctatggat atgcaaagat
    29761 agctaagtct gaaaaagaaa agtggggagg tataatggaa cgatatctag aaagtagaca
    29821 agagttatgt tctatattcc tacttgtaga tattagacat gagcctacag cagatgataa
    29881 gcttatgtat gaatggataa agcactttgg atataactgt gtagttattg caactaaggc
    29941 agataaaata tctagagggc aatatcaaaa acacataagt ataataagaa aaaaattgca
    30001 aatggaaagt agtgaaaaag taattcctgt ttcttcacta aaaaagactg gtgttgaaga
    30061 attatgggaa gagattgtaa atcaatacaa tcaacatgga tatgaaataa cagtagatta
    30121 aatttaaagt gttaaatatt gacataagat ttatttgaat aataaatgta ataatagtta
    30181 gttttaatta tattaatatt aaagcaaaat ataggatgcc ttaaaatagt tttaatgaac
    30241 tttttttggc atccttattg tttataaaca ataaatatca gtaattttgc taaatactaa
    30301 taaattttag taaggtatgg gaaaattagt ataaattagt tcaactaaaa aattattgac
    30361 ttatgtagtc agcaataaat agcatttaag aaatagattt atctgttttt ctcataaaga
    30421 aaacagttag tataagccct ataaaacata ctgatgctgc aatccaataa gactgattgc
    30481 tagctataac ataggctgat gtgtgacttt taaacttttg attgtatgta agactttgtg
    30541 caaaaaaact actaaaaaat gctgttccaa gagcaaatga taaattacga atagttgcaa
    30601 gaaaactgcc tccgtacccc tgtttttgag gaggaacatc tgctaaaata ctactattgt
    30661 ttggtgaact aaatgctcct agtccaagtc caaaaataac taaacataca attaaaagat
    30721 acaatggaga atctgctttt aaaagtgcca ttccaactag tgagatggtc ataaatgaaa
    30781 aagatgctgt tagaatgttt tttgcaccgt gtttatcaga aagtgaacct gcaataggtg
    30841 ctataagtac cataataagt ggatgtacag tcattacata accagccatc ataggagata
    30901 aattttttaa ttgttctaaa taaaatggaa gtagaaaaga tactgccatt tgaggaaaat
    30961 aacaagctac tccaatgata ttgcctacag taaagtttct atttttaaat agaggtaggt
    31021 caataaagct ttgttcaaat tttacttctc ttaatgccaa caataagaag attataagcg
    31081 ctgttatacc taaatacaat cttgtattac ccttctcatt cattgcaagt aatataagcg
    31141 tagtgcaaga tgcgaaaagt agaagacctg gaatatcaaa gctcttgttt tgttcatcca
    31201 aaacaggttt aggcagaaat ttaatgccaa gaaagactgc tataatacca ataggtatat
    31261 tgataataaa aatagaaggc catccaaaat gttctaaaat tatacctcca attacaggac
    31321 cactcatatt tcctatacca actacagctc cagtgattcc tatagctttt cctttttcat
    31381 ttgctggaaa tgcattggaa actataccaa gcccagtagc cattaaaata cttgctccaa
    31441 ctgcctgaaa gattcttgaa gaaattaatg ttgataaatt gtttgacata ctacacagaa
    31501 aagaacctat tgtaaatata aaaaatccta atgtgtatag acgatttgaa cctactttat
    31561 cgccaagttt tccgaaaaat aacatagtag ctgttacaac aagcatataa gctgttacaa
    31621 cccactgaat ttgtgacatg ttaattttaa agtcagcagc aattgtagga tttgcgatat
    31681 ttagtatact tccatcaaga gtagacataa atacaccaat acagatgata atcaatacta
    31741 tccatttttg tttcatgata acactccaat cttaatagat tttataagta cgatataatt
    31801 gtaaaataaa caacaaaaaa agcacctgta tattaggtac cttgaatgtg tctaatttca
    31861 tgaataaata cggtagttga aggatttgta tagattactc atgtaaaatt acataaaggt
    31921 actcatatac tattttcttc taccatccag attttactgt cggctttgga atatgaccaa
    31981 attatgccat aaggctcgcg ggcttttacc gccgataggg aatttcaccc gtttatgaag
    32041 aaaaacagat gtattttgta atattatttt aacataaaat tataatggaa acaattggat
    32101 ttaagtaata tccagctgta ttacaataca aatatttcaa tatggacact taaattgtat
    32161 ttatagatag aataaggcac agtatgagaa tctataataa ttagataaaa ttaacctgtt
    32221 attagtatta gatggcaagt tttaaatttg tcaaaatcag aaagctgtaa taaaattctt
    32281 ataatatgaa tatcttaatt aagagatatt gtaaattatg aggaggcgct aatatggaag
    32341 gaaaggtaaa gaaggcattc ccgggtggaa atacatcaca aggctcatat tctttttttg
    32401 actatataat tcctgaaaat gtaaaaagag ttttttgttt aaagggtgga cctggagtag
    32461 gaaaatcctc acttatgaaa aaaatttata aagagttttt gcatagagga tatgatattg
    32521 atttatatca ttgtccttct gacccaagtt cgttggatgg tttagttata aagaaattag
    32581 gcgtggtact tatggatgca actgccccac atactatgga cccaaagcta cctggagctt
    32641 tggatgagat aattaattta ggtgattact ggaatacaga agaattagaa aagaataaac
    32701 aagaaatatc agtttatgat aaagatatta gtaattcatt taaaagagca tttaagtttt
    32761 taaaatcagc agaaccaatc tttagagaca tagaagaaaa atattctgat tgtatggatt
    32821 atggcaaagt aaatttggtc acagaagaat ttataaaaag gctatttgat ggagttaaaa
    32881 gcacaggaaa tctaaaaaga gaaaagcatt tatttggttc agctataact cctgtagggt
    32941 gtttagatta tacagaaaac attttaaaag atgtaaagaa agtctattat ttagatggag
    33001 aaataggaac tgggaaaact acattattaa acaaagtata taagaaagct attgaaaaag
    33061 gtttaaatgt agaagtttat cattatccac ttattccaga gaaaattgaa actgtactat
    33121 taactgattt agatataggc ataactataa gtacgacttt taaagaagaa gatacaattg
    33181 atttaaatca gtttttaaat agagaaaaac tacttaaata tgaagaagat attaaatttg
    33241 atgaaaaagt tttagatgat ttaatttgtt gggcagtact aaatcttaag agagccaagt
    33301 ctaaacatga tattatagaa agttactata gtcctaatat gagatttgat gaagtaggga
    33361 aattaagaga taagttaatt aaaagaattt taaaatatga ggaaaactta taaacgcata
    33421 tgtttaaaat taacgttagt aataatataa tttttagtaa attggtgtag ctttgaggct
    33481 atgccatttt actttttttg ctaaaaaatg atattaataa tctccaaagc cattatttgt
    33541 ttttcataat tattatttcc tttcctaatt tatgtttttt tataatatca caacaatgtt
    33601 tattgaaagt ttatataatt ttaagaacat ctaaaaatgc atacttgata ttggtaaatt
    33661 agtgatagga gttcaatgta tgatattata tactaaagat gaattttaat atataacttt
    33721 gatataataa gcatatgtaa atgtttaata tttaattaaa gaaaattatc atataaaaat
    33781 aaatttgtaa ggagcaatac agtatgaaaa aacaatatgt gtttataata ctagcaagta
    33841 ctattttagg tatacttgtt ggaaccatat ttagagatta tagcaatgaa gaaacagttt
    33901 atacaagcaa agacaagtca ataagaaaag aaataaaaat gacaagaaaa tctataaaag
    33961 aattaaccaa agagaaagac aagttagata aagaaattaa ttcaatgaaa caaaatcaaa
    34021 aaaattctga agaaatatca gaaatagaaa cacttagaga tagtctatca tattcagatg
    34081 taaagggtag tgggataata ttaagtatag atgctataga tgaaaaaaca gggaatatag
    34141 ctaatagtat agactataat aagattcttg taaaagttat aaatgaatta aaagtaaacg
    34201 gaggaaaata catagaaata aatggtcaaa gaattaatca atattcagaa gttgtacttg
    34261 caggaagcca tataaatata aatggagttc ctgtagcaca accatatgaa ataaagtgca
    34321 ttggagataa ggagaaacta tctaattatg tagataaagg tagtaaatat ttagatagca
    34381 tacaaaccaa ttatccaatg aaaataagca taaaaactga agaaaatatc tctatgaaaa
    34441 aaatgaatat acctaataaa ttaaagtata taaagggtga ttaagattat attagaggaa
    34501 agcagtttgc caatgttaat accagaaaaa cttgcaaaaa aatataatgt atttccagtt
    34561 gagtttcaag atgataattt agttgtagaa atagaagatg aggatattta tgctctacag
    34621 gacttgagat tagctacagg taaagaaatt atattgaaga aggagaaaaa agaacttata
    34681 agtgaaaaga ttgaaaaata ttataatagc tttgaacttg atgaggatta tgcaaaaaaa
    34741 ttatttgaaa atcttttaga aaaagctata aaagaaaacg caagtgatat acatatagaa
    34801 ccatttaaag aacatttaat agtgagaatg agggtagatg gagaactaaa ggaagtatca
    34861 aagtttttag ttgatgtcta tccatcattg tctacggtta taaaattaaa ggcatcaatg
    34921 gatataacag aaaaaaggct tcctcaggat ggtagggtgg atataaagtt aggtggaaat
    34981 ttaattgaca ttagagtttc atcaatacca actgtctatg gtgaaaaaat agttttgagg
    35041 atacttaata gagatacttt tttaaaagac aaattagaac ttggtttttc agaggaagct
    35101 ataaaatcta taaatcgtat aataaataaa agagcaggaa tattattagt gacaggtcct
    35161 acaggtagtg gaaagactac aacagtatat tccttattaa atgatttaaa aggaataaat
    35221 aaaaatatta tgactataga aaatcctgta gagtataaga tggatggcat aagtcagatt
    35281 caagtaaatt caaaagtagg gttgagcttt gatgtaggtc ttagagcgat attaagacaa
    35341 gacccagata taataatggt cggagaaata aaagatgcag aaacagctaa gatagcagta
    35401 agagcagcta taacaggtca tttagttata agtactttac atactaatga tgcagtatct
    35461 tccatagcta gattactaga aatgcaaata ccaccatatc ttttaaatgc ctctcttata
    35521 ggtgttatat ctcaaaagtt ggttagaaaa gtctgcaatc attgtagcca tgagattatg
    35581 ataaaagata attttagtgg agatgtaaat actaaggtag ctgtagggtg tgaaaaatgt
    35641 aatgataaag gatactttgg aagaacagca atttatgaaa tcttagaaat taatgatgat
    35701 ataaagacat gtataagaaa tatggaggat tctagtacta ttaaagatgt ggcacaaaat
    35761 aatggaatga taacatttga agatagttgt aaaagactaa taaatgaaaa aataacgaca
    35821 ttagaagagt gtttagtagt aaatgaaatg atttaactga attgtatggt taaacttcgt
    35881 ttggtcagtt gtctttattt aatctatttt aattaagatg tttgagtata aattatattt
    35941 aagtgaaata aaattaaatg cattaaatta gaaaatatta agtttattta attgtaatta
    36001 atataatttg gaaggtatta tagtacatga gattatctaa aaatatattt tataatttag
    36061 aaaagtttta tttagtatta aatagaatca gtgataaaga aattagagta atttgcaaag
    36121 agataggcat attgctggaa tctggatgtg aaattacaaa aatatttgaa atcattgaga
    36181 gtcagtcaag taaaaaagta aagaatttgc ttagtatagt atcaaatcat attcaaaaag
    36241 gtaactca
//
LOCUS       DAGSHT010000017        36031 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.018, whole genome shotgun sequence.
ACCESSION   DAGSHT010000017 DAGSHT010000000
VERSION     DAGSHT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.018"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..651)
                     /locus_tag="MJB72_000818"
     CDS             complement(<1..651)
                     /locus_tag="MJB72_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBY8261943.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINF"
     gene            817..3384
                     /locus_tag="MJB72_000819"
     CDS             817..3384
                     /locus_tag="MJB72_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8261944.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3614..4018
                     /locus_tag="MJB72_000820"
     CDS             3614..4018
                     /locus_tag="MJB72_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8261945.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4042..4539
                     /locus_tag="MJB72_000821"
     CDS             4042..4539
                     /locus_tag="MJB72_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8261946.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4542..4943
                     /locus_tag="MJB72_000822"
     CDS             4542..4943
                     /locus_tag="MJB72_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBY8261947.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            4961..5449
                     /locus_tag="MJB72_000823"
     CDS             4961..5449
                     /locus_tag="MJB72_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8261948.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5476..6231
                     /locus_tag="MJB72_000824"
     CDS             5476..6231
                     /locus_tag="MJB72_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8261949.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6231..7094
                     /locus_tag="MJB72_000825"
     CDS             6231..7094
                     /locus_tag="MJB72_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY8261950.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7141..7620
                     /locus_tag="MJB72_000826"
     CDS             7141..7620
                     /locus_tag="MJB72_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBY8261951.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            7685..8992
                     /locus_tag="MJB72_000827"
     CDS             7685..8992
                     /locus_tag="MJB72_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8261952.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9302..9442
                     /locus_tag="MJB72_000828"
     CDS             9302..9442
                     /locus_tag="MJB72_000828"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBY8261953.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            9952..11046
                     /locus_tag="MJB72_000829"
     CDS             9952..11046
                     /locus_tag="MJB72_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8261954.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            11051..11251
                     /locus_tag="MJB72_000830"
     CDS             11051..11251
                     /locus_tag="MJB72_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBY8261955.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11251..12168
                     /locus_tag="MJB72_000831"
     CDS             11251..12168
                     /locus_tag="MJB72_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8261956.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRILLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12165..12923
                     /locus_tag="MJB72_000832"
     CDS             12165..12923
                     /locus_tag="MJB72_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8261957.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTVAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLVAVILFNKKPV"
     gene            13182..14255
                     /locus_tag="MJB72_000833"
     CDS             13182..14255
                     /locus_tag="MJB72_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBY8261958.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            14325..14588
                     /locus_tag="MJB72_000834"
     CDS             14325..14588
                     /locus_tag="MJB72_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261959.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            14794..15771
                     /gene="bioB"
                     /locus_tag="MJB72_000835"
     CDS             14794..15771
                     /gene="bioB"
                     /locus_tag="MJB72_000835"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBY8261960.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            15999..17012
                     /locus_tag="MJB72_000836"
     CDS             15999..17012
                     /locus_tag="MJB72_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY8261961.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17054..17944
                     /gene="rbsK"
                     /locus_tag="MJB72_000837"
     CDS             17054..17944
                     /gene="rbsK"
                     /locus_tag="MJB72_000837"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBY8261962.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            17992..18954
                     /locus_tag="MJB72_000838"
     CDS             17992..18954
                     /locus_tag="MJB72_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBY8261963.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19008..20519
                     /locus_tag="MJB72_000839"
     CDS             19008..20519
                     /locus_tag="MJB72_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBY8261964.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVTQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            20503..21501
                     /locus_tag="MJB72_000840"
     CDS             20503..21501
                     /locus_tag="MJB72_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBY8261965.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGSSFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            21685..22947
                     /locus_tag="MJB72_000841"
     CDS             21685..22947
                     /locus_tag="MJB72_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8261966.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMNKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            22967..23710
                     /locus_tag="MJB72_000842"
     CDS             22967..23710
                     /locus_tag="MJB72_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBY8261967.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGSKEFTDWKYYNKLWVNMLDWLTC"
     gene            23883..25268
                     /locus_tag="MJB72_000843"
     CDS             23883..25268
                     /locus_tag="MJB72_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8261968.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTSMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNENDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKY
                     TVGSFEYTPII"
     gene            25829..25969
                     /locus_tag="MJB72_000844"
     CDS             25829..25969
                     /locus_tag="MJB72_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261969.1"
                     /translation="MKNENISYLALGLCFGVILGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(26199..26645)
                     /locus_tag="MJB72_000845"
     CDS             complement(26199..26645)
                     /locus_tag="MJB72_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8261970.1"
                     /translation="MIKFEKLNIINNKDMKEILDTWESSVRATHDFLSEEKIISIKPQ
                     VKEGAKYVSNFLCVRDKKGLIKAFMGIHDFKIEMLFVGNYSIGNGIGKRLAEYAIEVL
                     NVNYVDVNEQNQQALGFYKHMGFKVFKKSEFDEQGNPFPILHMKLK"
     gene            27415..28254
                     /locus_tag="MJB72_000846"
     CDS             27415..28254
                     /locus_tag="MJB72_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261971.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            28244..28687
                     /locus_tag="MJB72_000847"
     CDS             28244..28687
                     /locus_tag="MJB72_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261972.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            28698..29189
                     /locus_tag="MJB72_000848"
     CDS             28698..29189
                     /locus_tag="MJB72_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261973.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(29391..30119)
                     /locus_tag="MJB72_000849"
     CDS             complement(29391..30119)
                     /locus_tag="MJB72_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBY8261974.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            30244..31332
                     /locus_tag="MJB72_000850"
     CDS             30244..31332
                     /locus_tag="MJB72_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261975.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKESMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(31769..32617)
                     /locus_tag="MJB72_000851"
     CDS             complement(31769..32617)
                     /locus_tag="MJB72_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261976.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            32816..33181
                     /locus_tag="MJB72_000852"
     CDS             32816..33181
                     /locus_tag="MJB72_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8261977.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            33204..35591
                     /gene="cadA"
                     /locus_tag="MJB72_000853"
     CDS             33204..35591
                     /gene="cadA"
                     /locus_tag="MJB72_000853"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBY8261978.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEIKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        14085 a         3680 c         6539 g        11727 t
ORIGIN      
        1 aaaattaatt tcctttaaga tattttcaga atactcatag ttacctatgt caaaacaaat
       61 gtgagcatct gaagttagta tgattttagc accatatttt ttgcaaagtg tagcaatata
      121 cttacagtta tccgagctac catttcttga cacgccttta atagaacaat tatttatttc
      181 tattaagata ttcttttcta tagctaattt aatgattaat tcatagtcga ttgggtagtt
      241 tgggtttcca aggtgtccta atatttcaat attatcatgc ttatttattg catttattaa
      301 agctttagta ttattctcta atgtatttgg agaaaaaaca gcttcgtgaa atgacaagat
      361 aagataatct aaccgaggaa ttacaaaagg ctctaggtct atgtttcctt taatatctag
      421 tatatttgct tcacagcctc ttaaaattat aatgttattt atgattcttg ggatattttg
      481 aatgttgtga aagtaccatc tgtggggaga accaggcatc ttaggaccat gttcagatgt
      541 acctagtatc ttaattccat tttctttaca ataattaata ttttctagta aagtgctata
      601 gccatgacca ctgactatag agtgggtgtg taaatctgat aaaaaattca tataaattcc
      661 tcctctataa aagatgaggg taacgtaatt tatattataa aacgaatagg aaagaaaatc
      721 aatagttaca taattagaca aaaatattaa aatattgtag aattaagctt aataataaat
      781 gtttaacagt attactgctt ataggggagg ttaactatga cacaaaagaa gctatcaagg
      841 aaagaaaaag ttttaatttg cttgaaaagg ttaacagaag aacacttgaa ctttaataat
      901 ggcattagat ttggatttga tgcagaatat atagctactg aagtaggtat aaatagaagt
      961 aatgctagta aggaattaaa tcaattaata aaagaaagta ttattataaa gataaagggt
     1021 aagcctgttc agtacttata taaagaaagt attgagagta ttttaggaaa gaaactaaat
     1081 aaagggatat ttgataatat taactctata aacgaatata gtgaaaatga ttctatcaaa
     1141 gatgagtata ttttagagag tataaaaaaa gagaacaata tttttaattt agtaggatgt
     1201 gatggaagtt taaggtccca aatagaacag gctaaagcag cagtaatata tccacctaag
     1261 gggttaagta cattaataat aggacctaca ggagttggta aatctatatt tgcagaatat
     1321 atgtataagt atagtttaac tataaaagaa gatagtgata atgcaccatt tatcatattt
     1381 aactgtgctg attattctga taatcagcaa ttattattag cgcagctttt tggatatgta
     1441 aaaggagcat ttactggagc tgataaagat aagtatggta ttgtacatga agctaataat
     1501 ggagtattgt ttttagatga agttcataga ttatctgcag agggacaaga aatgctattt
     1561 ctgcttatgg ataaagggat atatagaagg ttaggagagg ctaataaggt acacgagtgt
     1621 agagttatga taattgcagc cactacagaa gacccagaaa cagcaatgtt ggaaacgttt
     1681 ttaagaagaa tacctgtaac tataaagatt ccgtctttag aggaaagagg ctttcaagag
     1741 agaatgaagc ttatatgtta tttttttaaa gaagagagtg ttaggattgg tgttaagtta
     1801 aaggtttcaa aagaagttat taaggcattt attttatata agtgtaaggg aaatataggc
     1861 caattaaaaa gcgatataca attaatttgt gcaagagctt ttttagatta tatgacctat
     1921 aaaagggaat gtgtatatgt aaggctctct cttctaccaa ataatattag agaatcatta
     1981 tatggaaata atagaaaaga ggagtttgta caaaatttca gttttattgg aaaagatgat
     2041 attatttttt taccagaaga aaaggatttt gaaagtgaaa atttattaat agaaaataaa
     2101 aaatatgatt tagattttta tggaatgata aaagaaactt ggaataaact tcaacaagaa
     2161 ggtattaaag agtcacaaat tagaagtgtt atagatggag acatacagaa gtattcctat
     2221 aatttaatga atacttttat atataatagt agtaatcaaa cagcttataa caacattgtt
     2281 aatgattcaa ttgcaactac tgtaaaatat attttacaaa agcatgataa atggagcaaa
     2341 agagaaggat tagataaatt aataaaagct atagctttac atattcagaa tttaattgaa
     2401 agaataaaga ttggaaatat agtaaagcat cctaataaag atgaaattat gaaggaaaga
     2461 gcatatgaat ataacatagc aaaagaaata ttatctaaca tgtcattaat gtatggagta
     2521 gagtttccag aagatgaagc aattttttta gcaacatttc tttatttatc gtatgtggga
     2581 ttaaatgagg agagtatagc aatattagtc ataatgcatg gtgaatctac agcatctagc
     2641 atggtgaatg tagccaatac tttactggat tgtaaccatg cagtggcaat taatatggga
     2701 ttagaagata gagttcagga tattttagta caagctgtag aaatagccaa taaaattgat
     2761 aaaggaaagg gaattttaat tttaactgat atggggtcta ttttaacatt tgctaaagtt
     2821 attcgtgatg ctacaggaaa agaagtaaaa gcaatagata tggtaagtac acctattgta
     2881 atagaagcaa ctagaaaggc tctaactcca gaaatgacat tagagaaatt atattttaat
     2941 attatagaat ctataaagaa acattatgga gatatggaag ttacatatgc tgaaaaagat
     3001 aatggtggta gatattttga tagattacta attgataata ttagtaaaac tttgactttt
     3061 ttaaatgggg aaaaggctta ttttatatta aaagaagtaa ttaatagaat tagtgaacac
     3121 tattctttag taattcaaga tgaactttta gtaaagttta tatttcattg ttcatgtatg
     3181 attgaaagag taataataaa agagtcttta gtttataaat catatgaaga aagaattagt
     3241 agaaataaag aattatattt agttataaaa gaaagtttta agctagtaga agagacattc
     3301 gatataatta tttcagatat ggaatatgca aatatattag atatatttga atcacaatat
     3361 gatacagata aattcaaaag ttgatacaaa atttaaaaat agccaaaagg ctatcttttt
     3421 tttacttttt ttgatacaga tttgatacag gttgaaaaaa tcttttgata catatcgaaa
     3481 taataagttt ttaaaaaaaa ttgaataaaa agttttgatt aaatcctgta aaatagccat
     3541 ttgttattga tacacataag ttggcacggc aattgcttat ataaaaagtg acaagaaaag
     3601 tgaggtgaaa ttggtgatag gaataatagt tatgactcat gggagtttta gtgaagaaat
     3661 tataaaaagc tgtgaactta ttgcaggacc agcagaaaga acagcagcaa ttaaactaaa
     3721 taggaatgat aatattgaag acttaaataa aaattttgta gaaaagttaa atgaattaga
     3781 tgaaggtgac ggagttttag tattagctga cctattagga ggttcgccaa gtaatgttgc
     3841 aagtttaaac cttaaaaaag gtggcaagtt tcatgcactt actggagtaa atcttccaat
     3901 gctacttgaa gctttaatta atagagaggg aaaaagttta gaagagttag cagaagcatg
     3961 tattgaagca ggacaaactg gtataaacaa tattaacaaa gttcttagct caatgtaata
     4021 gaaaaaggag tttgattaaa aatgtcttta gtaaaatatt gcagatgtga tgatagatta
     4081 atacatggtc aagttatata taaatgggtt aagcatctag gtgtaaaaaa gatagtagta
     4141 gtagatgatg aaacaaccaa tgatgttatt gctaaaggtt tgataaaaat ggcagcacca
     4201 aagaatattg atttatcaat attaactgta agtgaatcaa ggagatattt ttataataat
     4261 caagctgatg ataacgtttt tgtattaatt aaaaatttag atactgcaaa tagaatgata
     4321 gaagaaggga ttaatatcaa aaaacttatt gttggaagaa tacctacagg aataggaaaa
     4381 aagaaaatat ctcaaaatgt atacattaat aaaaaagagt ttcttttaat agatgaattt
     4441 attaaaaaga atattaatgt atcaattcaa atggtacctg atgaagaaga agtagaatta
     4501 aaccaaaata ttaatcgcta taaaggagag tttatttagc aatgaataaa tataaggtaa
     4561 tcatactgtg ccatggaact tggggaaaag tcttagtaga agaaacaaat aaaaactttg
     4621 gattgtcaaa tcaatatgaa gttttatcac taagttctga aaaagatgtt tctgtattta
     4681 tgaaagaggt tgaagaagta gtagataaag aagatgtgaa aattataata tcagatttat
     4741 atggaggatc tacatcaagt gttgcaatag ctgttgctat aagaagagga atagatgcaa
     4801 taaatggact tagcttacag acaattttaa tcgtagatga agaattaagc aaagaagata
     4861 aattacatac attaccagaa agaatcgtca aaaaaaataa taacctttgt gtggatttaa
     4921 ttaaagaatt taaaaaaata taaattatga ggtgaatata atggcaaaaa taacattagt
     4981 aagagtagac cacagattaa ttcatgggca agttataaca aaatgggtaa aaatcgctca
     5041 agctcaaaaa ataataatag ttgatgactt cttaggtcaa gatgaattta tggctgacat
     5101 atataaaatg gcagcaccat caggagtaga agtagttatt ttaacagcag aagatgcggg
     5161 acaagcattc caaaacaaca ctttaggaga taaaaatata tttattctat ttaaaaatgt
     5221 tgatatggca aataaggctt ataaagctgg acttaaatat gaaaagattc aattaggagg
     5281 tattccaaat gaagcaggaa agaaaatggt gtttacagct gtttcattag gaaatgagga
     5341 tgtagaacaa cttaatgaac ttaatgaaaa tggggttgaa atagtcctac aagttatacc
     5401 agaggaatca tctatgacat atgaaaatgc gttaaagaaa tttaaataaa aaatataaat
     5461 taggaggaat tataaatgga atctgttttg atgttggcaa ttgtaacagg attatggtat
     5521 tggtttgcag caggtcttgc agggtatacc cttttctcaa cactaaagtc tcctttattt
     5581 ataggtttta gccttggatt actttggggt gatgtaacaa ctggtatgat tgttggtgca
     5641 agtatagaaa tggtttatct aggaatggtc gcagcagggg gaaatattcc atcagataaa
     5701 tgtctagctg ctcttattgc aataccagta gcacttcaaa ctggtgttaa tgcagaagtt
     5761 gcagtatcta ttgcggtacc tttaggtgtt ataggagtat tagttaataa cttaagaaga
     5821 actggtaatg ctgtattggt tcataaggca gataagtatg ctgaagaagg taatacaaaa
     5881 ggcatctgga gatgtgcaac actttattct ttaatatttg ggtttgtttt aaggtttcca
     5941 atagtttttg tatgtaactt ttttggagca gatttagttc aatcattatt agatgttata
     6001 ccacaatggt taatgaatgg attaacagta atgggcggta tattacctgc attaggtttt
     6061 gcaacaacaa tatttactat aggtaaaaat aaatttttac caatgtttat tataggattt
     6121 tttatggttc aatattttga aatatcaatt actgcagctg caatatttgg tgtttgtata
     6181 gctcttttaa ttacctttat gaaagaagac aaaagggtag gtgaagtata atgcaagaaa
     6241 taaaaaataa agaagttaaa aatggacaag gtccaactga agttaataag acaagagtac
     6301 ttacaaagaa agatgtaaca aaaacatatc taagatggtg gtggacagca gaattatcca
     6361 actcttttga aagaatgcaa gcattagcag tttgtgcaag tttcactccg gctttagaaa
     6421 agttatataa gagaaaagaa gatttagtag atgcgttaaa gagacactta caatttttta
     6481 atactcaagc tatatgggga ggattaattc acggtacagt tttagctatg gaagaagaga
     6541 aagctactga aggaaaaatc ccaggagaag ttatttcagg tgttaagaat ggtcttatgg
     6601 gacctttggc tggaattgga gatactttag attttggtac atttcaaaca atattcttag
     6661 ctttaggtgc ttcatttgga gcagaaggaa gtgttatagg tgcatttttc ccaataatgt
     6721 ttagtatatt actattttgc gaaggttatt acttatttca tttaggatat tctttaggta
     6781 gagattcaat taagaaaatt ctatcaggtg gtattgtcaa taagataatt gatggtgctt
     6841 caattctagg aatgtttatg atgggagcat tatctgcaac tacagttaag ctttcaactc
     6901 cgctatcatt tgatattggt ggtaaagcaa ttgttgttca agatacatta aatatgattg
     6961 ctccaggttt attaccactt ggtgttgtat tcttcgtata ttggggaatg aaatacaaga
     7021 agtggacaat aactaagtta ttagtaattt tagttgtatt agcattggta gggtcattca
     7081 ttggaatatt ctaattcaca gtgattaaaa tatagaattt aattttagga ggagaaagaa
     7141 atgtcagaaa aaatgtatac agaaaaggaa tatttagaag gaattagagg agcaacagga
     7201 gaaagagcag tatggttcta tcttcttatg aaagaagctg aaaaattagg agctaatcca
     7261 gatgatatat gtaaagaagc aatatatgga tttggtaaaa tgagaggtca aaaatataac
     7321 gtagcagata ctccaggaaa aatggcagaa atgttatata acagtaaagg acaaaaagtg
     7381 tttgaaatgg aattagtaga aaatacagat gagaatggcg ttttaaaatt ccatcactgt
     7441 ccattagatg ctgcttggaa agaatatgga ttaactaaag aagaaagaaa agaaatttgt
     7501 agattagctt gttatgggga ttatggaaga gttgactgcg cacaaggtgt taaattagat
     7561 tttgctcaaa aatgtgctca tgatgatgaa gtttgtgaat tagtatttac aaaaaaataa
     7621 tttttaatgc taaaagcctg ttctttaagg acaggcttta aaaagataga aagtaggttg
     7681 atttatggat ataagcagaa gtgtagataa tagtttaaat agaaccatag aatttctaaa
     7741 agaattgata aaaattgatt cacaacaagg tgaacctatt tcacaatgtc cttttggtat
     7801 aggacctaaa aaatctttag ataaaacttt agattactgt gctagtttag gttttagtgt
     7861 taaaaatatt gataattata ttggatatgc agaaattgga gagggagagg aattaatagg
     7921 gattccaatg catttagata tagttccacc aggagagggg tggagtgtag acccattttc
     7981 gggagcagta attgacaata ttatttatgg aagaggtgta atagataata agggtgctgt
     8041 atctatgctt atacatgtat tgaaaaacat agaggatatg tatcctacaa ttaataaaag
     8101 aataagatta atttttggta caaatgagga gacaggtatg aaatgtatca aatattactt
     8161 agataaaggt gaagaaattc caagtatggg atttactcca gatgctatgt atccagtggt
     8221 taatggagaa aaaggaaggg ttcatataag gatagaaaaa gaaattaaaa ttgacaaaag
     8281 taaaccttac ataatagtaa ggggaggaac taaagaaaat gttgttccat ctcattgtac
     8341 tgcaaaaatt attaatggaa ttatttcaga gttaactact aaaggagtag ctgtacatgc
     8401 aagtaatcct gaaaagggag aaaatgctat atctaaaatg gtaataaaga tagttgagga
     8461 taatatggat tttcagcata gagaagatat cgaattagtt tcaaaatacc tttgtagtga
     8521 ttattatgga gatgcattag ggattaatca atatgatgag gtattcaaaa acactacctt
     8581 aaatttagga atattaaaag taaatgagga aaaaatcgta tgtgagttag atataaggta
     8641 tggtaaaaat atagttttaa acaatataat agatagattc aaaaaggttt tttgtaatgg
     8701 ttggaaaata gaggttattg ctcataaaga cttacattat gtggatgaga gtaatttagt
     8761 acttaaaaaa ttattagaag cctatgaaga agtaacagat gaaaatggat atacaattgc
     8821 tatgggtggt ggcacatatg caagttggtt taaagatatg gtggcgtttg gacctaagtt
     8881 cttagcgtat aaaactggtg gtcatggagt agatgaaaga gtaccaataa atcatatacg
     8941 aaagaatatg gagatatata ctttggcttt aataaaacta ttagaattgt aaaagaagaa
     9001 gagactttat gtgcaaattt acagatatct taaaaagaga gataaaacga gttcaacctt
     9061 atttaaatgt tcaacaccat caaatggggc tgtctcaaaa tagagataag cctcttactt
     9121 attgttgaaa tgatgaaaat atattttact tcatacaaaa aagagtgcct tatgaagtaa
     9181 aaagttaatt ttgagacacc ctcatttgat gatgttgaag agaaaataag tatgaaatta
     9241 ttaaaaaaga tgattttata aaagttaagg ttgaaaagaa aagttaattg aatggaatta
     9301 gataaatgga gaaggatgta tactatctaa taaaataaca aaagagtgtt ataaagttgg
     9361 ttatatgatt agagaaaagt ctattgatac ttatccagat agtggatggg gatttatgac
     9421 ttgggatgaa aatggagaat aataaaaatt atagtttata cagtttaata agatatatgt
     9481 acaagcagtt aatctaaatg attgactgct tttcttttat agatttttat ataatagaca
     9541 aataccaata catgatagtt gcctgttata attagagaaa taaatacgaa taataacttt
     9601 ataaaagaat taaatggaaa atcgaagagt tatattcagt attttaaaaa gaatatgaaa
     9661 tgagaaatga aaataaggtg tttatattaa aagaggtttt acacaaaata tttaaaaacg
     9721 tgataaataa aaactgtatt tagatagatt aactattagt tttatataat atggatttta
     9781 tttacaaagt ttacatattt tacaaaggag attaaagata aatttaaata tatattttat
     9841 atacttgttt atttgtttct ctgtggttga ttttttcgtc aaaagtcaac ttcaaatcgc
     9901 ttgttagctt tacatgttta aggatataat tatacataag gagggattga catgaatgaa
     9961 ttaaacatcg caaaaacgct gattttaaag cgtaaagaaa aaggaattac acaagatgaa
    10021 cttgcaaatt atataggcgt ttctaaagct tctgtatcta aatgggaaac tggacaaagc
    10081 tatcctgaca taactttttt accgcaactc gcgacatatt tcaacattac cgttgatgag
    10141 ttaatatgtt atgaacctca aatgatgaag gaagatatta ataaactgta taataaactg
    10201 tgcaaagact ttacggttaa accatttgat gaagtcatgg atgaaataag agaaatcata
    10261 aaaaaatatt attcatgttt tcctttgatt tttcggatgg gtcttttaat agttaatcac
    10321 tatgatattg tagatgaaaa aaagcgtgaa ctattaatta gtgaagcatt agaaatattt
    10381 attcgtatac aggaaacttg taatgacata gatatttgcc gtcaagcaaa aagtatggag
    10441 gcaacttgtt atatattgtt aaatcaaccc atacaagtga ttgatttatt gcaaaacagc
    10501 aattttccca tgataaatga atctatactt cttgcacagg ggcaaatgat gaatggccaa
    10561 atggatgaag caagagaaac atttcaactt ggtgcatatc agaaccttat atcattagtc
    10621 caaaatctag tgggtatttt gcaaaatgca gataaattac aaatgaagga aatagaaaga
    10681 cgtattctag caatttcaga tatatttgag cttgatacat tatctcctgc tattatgctt
    10741 tcttcatatt taacacaggc acaaattaat ttaatacatg gtgataatga aggagctatt
    10801 aaatcattac gaaaatatgt agatttagca acacgtgata tatacccaat tataatacat
    10861 ggggatgact tttttaataa acttgacaga tggatttctg aaattggaac aggtattaca
    10921 cgtgatgata caatcgtaaa agcaggtatt gttgctgcta ttaaaaataa tccaatgttt
    10981 tctgtattaa gtgaaaacaa agaatacaaa tttcttatag aaaaattatc tttattggag
    11041 gaataagatt atggaaaatt tgatggaata cttaccactt ttggttcctg ttattatttt
    11101 agatttaata cttataatta ctgcacttgt tcatgtgttg agacatccaa attataaaat
    11161 tggaaataag gcaatatgga ttattgtagt actgtttatt agtctaatag gaccaatttt
    11221 gtattttaca ataggaagag gtgaagaatg atgaatattg tctcaataaa tggattatca
    11281 aaaggttttg gtaatcgaaa gataatagat aatttaaatt ttactgtacc agaagggtca
    11341 gtctttgggt ttgtaggtaa gaatggtgca ggtaagacta caacaatgaa aatggtactt
    11401 gggttactta aaccagatag tggaactata gatgtttgtg gtgagaaagt gacctatgga
    11461 agaacaagtt ctaatcgtca tgtaggttat ctgcctgatg ttccagagtt ttacaattat
    11521 atgagaccac ttgaatatct ttctctttgt ggtgaaatta cgggactttc aaaaaaagaa
    11581 atacagataa ggagtgaaga gttgttatca cttgtgggtc ttggaaatga aaagagacga
    11641 ataggtgggt tttctcgtgg aatgaagcaa cgacttggta tagcccaagc tttgctttca
    11701 cgccccaaat tacttatttg tgatgaacca acaagtgcac ttgaccctgt tggtcgaaaa
    11761 gaaatattag atattatgct taaaatcaaa gactcaacaa ctgtaatatt ttcaacacat
    11821 atactatcag atgttgaaag aatctgtgac catgttgcta tattaaacaa aggaagcatt
    11881 gcccttagtg gtacactttc agaaataaaa agtatgcacg gaaaggatag aattttactg
    11941 gagtttgcaa gtaatgatga aattcaaaag tttaagtcta gtgatggtat taaatcctta
    12001 ctaaaagatt cagaagaaac taatatggaa attgttttgc atggtaaaga tataaaagca
    12061 atacaaaaaa cagttatatc tacactcgca gaaatgaatc tttgtccagt aaagatggaa
    12121 ctaatagaat tatcacttga aaatttattt ttggaggtag taaagtgaga gaatacatag
    12181 catttacaaa aaaagaattt aaagagaatt taagaaatta taaattattt agtttaatta
    12241 ttttatttct aatatttggt ataagtagtc ctatttctgc aaaatttatg ccagacttga
    12301 ttgctcattt tgcaccgact ttaaaagtta ctgttgcacc aactgcttta gattcttgga
    12361 cacaattttt tagcaatatc tctggtcttg gtatgagttt aactctaatt attttttgta
    12421 atattttatc aaatgaatat tctaaaggga cacttgttat tatgctgact aaaggtttat
    12481 cacgttcatc tatagtattg tctaaattct ctgttacggt gattattatg acaattggtt
    12541 tttggttgag ttttttattt gcttatggat atacaatgta tttttggcca actgcaaatc
    12601 ttaatcatat tatattttca gcatttaatt tatggcttat aggaattatg tatattagtg
    12661 tattaattct tgggtgtgta ctatttagac cagcatttgc cagtgtttta cttgtattgg
    12721 tggcaactct tttattgagt ttaatttcta ttcctaaaca gattgcacct tatactccta
    12781 atttcttgat gtcaaaaaat atagacttga tttcagggaa agtggcaatg ccagaattta
    12841 ttattccaat tattataaca gttcttattt ctatagtatg tttactagta gctgttattt
    12901 tatttaataa aaaaccagtt taataatacc tttatcacaa ggtaaagtaa tcttatgttg
    12961 ttaaaaacta cacctaaaaa tcaaaaagac ttggacttaa gtttcaagtc tttttgattt
    13021 ttaggtgtag tttaatatat agaattaagt gtagaaaata atacaaataa tattatttgt
    13081 atcaaataaa tataagaata tttaattatg gtaaaatata ctaaaaatta ctttaaaagt
    13141 gataaaatta gtataagtaa atattttaaa ggggtgtaga tatggaaaag tcaaaagtat
    13201 atttttgtga tttatactca aattcgcaaa ataaaaacgt accaaacaat gtaagaagat
    13261 tatttgatga agcaggattc aaagatttaa tagaaaaaaa tgaccaagtc gcaattaaac
    13321 ttcactttgg agaaaaggga aatactacat atatgagtcc tgtggcagtt agacaagtgg
    13381 ttgataaggt taaagactgc gaaggaaagc catttttaac agatacaaat acactgtata
    13441 cagggagtag aacaaattct gtagaccatc ttacaactgc tatagaaaat ggatttgctt
    13501 atgcagttgt aaatgcacca gtaataattg cagatggatt atatagtaga aattatgaga
    13561 atgtaaaaat agacaaaaaa cattttgaaa gtgtaaaaat aggtggagaa atttataatt
    13621 cttctgcaat gatagtaatg agtcatttta aaggtcatga agcagcaggt tttggaggag
    13681 cattaaaaaa cttagctatg ggatgtgcaa gtgcagcagg aaaacaaatg caacattctg
    13741 atgttactcc agtagtaaag gaaaagaagt gtgtaggttg tggaaagtgt gttaattctt
    13801 gtcctacaaa agctataagt atagtggata aaaaagcagt tatagattct gatgtatgtt
    13861 atggatgtgg agaatgtcca actgtctgtc caactagagc agtaactata caatgggaaa
    13921 gtgactcaga tgtgtttgtc gaaaagatgg cagaatacgc ttatggagca gtatcaaata
    13981 aaaaagacaa ggtagggtat ataacttttg ttatgaatgt aactccactt tgtgattgtg
    14041 taccttggtc aggtagacca attgcccatg atataggaat attagcatca acagaccctg
    14101 tagctattga acaagcatgc tatgatttga tttgtaagga aatgggtcat gatgttttca
    14161 aacatgaaca tcctcatgta aatggaacta gaataataga ctatgcatgt gaaatgggta
    14221 tgggaagtaa agaatatgaa ttaataaaat tataagaata gtagcactta aatgcatata
    14281 attattgtaa gagaaatcta aaagtatcaa aggagtatgc atttatgaaa atttactatg
    14341 agaaggaaaa cagcaagaag gctaatgtaa acaatattcc aagtgtattt gcgtttaatt
    14401 ttctaagaaa ctttttgact agcaaagagt atgaggaaac tagaaaagaa tattttataa
    14461 ataatctaac aaaaagtcag gttaatcaag ccatgaagga tttaaaatgg ctatttagag
    14521 aatatgaagg cttagaggtt attacaatgg aaagcttgga tggagttaag acaaggatag
    14581 ttttataaac cagtaagaat tatattctaa taaaaaatac tgctttaata ataatatgta
    14641 taatctatat actaatattg ttttgaagca gtattttttt gtgtatagta aacttgtgtc
    14701 taaaataaag ttgaccaata aatgttatat atgcaatact atatataata agtaaactac
    14761 ataaaaatat taagttgaca aataggagga tatatggaaa aatacataat aaaattaaaa
    14821 aataaagtat tgagagaaaa agaaataagt tatgaagaag ctcttaattt aataagcctt
    14881 gatacaaata ataaaaacga ttttgatatt ctattaaaat ctgcaaatga aataagagaa
    14941 tattttatgg gaagaaaagc agatttatgt acgataatga atgctaaatc tggcaaatgt
    15001 agtgaagact gtaaattttg tgctcagtct tcttattata aaactggtgt agaagaatat
    15061 tcattattag attacaatga aatcttaaat agagctaaag agatggagag taaaggagtg
    15121 catagattct cattggttac aagtgggaaa ggtatgtcag gaaaggagtt taataatatt
    15181 ttaaatatct atgaaggatt gagggaaaat actaatttaa aattatgtgc atctttaggt
    15241 attatagatt atgagcaagc taaaatgttg aagtcagcag gtgtgactac atatcaccac
    15301 aatgttgaga cgtgtagaga taattttcat aatatatgta caacacacac atataaagat
    15361 aggataaaaa ctataaaaga tgctaaaaaa gctgggctag atgtatgtgt aggcggtata
    15421 attggaatga atgagagtga agaacagaga ctaaaaatgg cttttgaaat acgagaatta
    15481 aatgtaaaat catttccaat aaacatttta aatcctatta agaatacacc aatggaaaat
    15541 tatgatgtat tagaaccaat ggaaatacta aaaacaacag cagtatttag gtttataatt
    15601 cctaatgtgt acataaggta tgctggtgga aggctatcat taaagggata tgacaaagtt
    15661 ggatttaatg ggggagtaaa ttctgcaatt gttggtgatt atttgacaac tgttggaagt
    15721 gggattgaaa atgataaaaa aatgattata gagcaaggat ttgaacttta ataaatacta
    15781 cttaaattaa ttaatatact tttagataat tataaaagta tattaatttt ttttgcagat
    15841 taatttaaat attaaaattg aattataagt tgcaatattt tgaaaaaagg gaaaaatatc
    15901 aaaatttata taaaatggtt gcaaatggaa aaaagatgtg atagtatcta ggtaaccggt
    15961 taagtaaaac ggtttcctaa attttggggg tgaacttctt gagtataact ataaaagaaa
    16021 ttggcgaatt ggctggtgtt tctaaaacaa ctgtgtcaaa agtcattaat aataaagatg
    16081 aaaatataag tcaggcaaca agagaaaaaa ttttgaaaat aatgaaggaa aaaaattatg
    16141 taccaaataa attagcacaa agtttagtaa ctaaaaaaac taatactata gggcttctta
    16201 taccagatat acgaaatcca ttctttacag atgtatcaag aggtgtagaa gacaaagcta
    16261 atgaagaagg ctacaatata attttatgta atacagatga agatgctaaa aaagaatatg
    16321 aaggaataag aacactatca gagaggatga tagatggaat tatatttgca gcatcttcaa
    16381 atacaaactg gaaagaagca aattataaag atataaaaat accaactgtc ttgattgata
    16441 agaagataag tatgaacaaa gaaaccttaa aaggaattgt taaaataaat aactttgaag
    16501 gggcctatat agctacaaaa catttattag atattggaaa taagaaaata atatacctaa
    16561 gtggtccact tcaaaatgaa atagcagtag atagattaga aggatataaa aaggctctaa
    16621 tcgagtgcaa tttaagctac aatcctgctt atgtatttga aggaaaatat aagattgaat
    16681 ggggacaaga atttataaaa aatttagaaa agatagattt tgatgcaata ttttgtgcaa
    16741 atgatttgat tgcaataggt gtcataagag gtcttaaaga aagaggatta agtataccaa
    16801 atgatataag tgtcgtggga tttgatgata ttcaaacatc aagtctaata agcccttctc
    16861 ttaccacagt aaaacagccg tcttatgata taggatataa agccagtgaa atattgataa
    16921 attgtttaag aggagataaa aaagaatcgt ttgatgaatt gattttcaaa ccagaattgg
    16981 tcataagaga ttcaacaaaa gaaagtgaat aacagcacga gagacataaa tagatatgta
    17041 aaggagtaat gtaatgggaa acatagttgt cataggaagt gtaaacatgg acatggtatg
    17101 ttctgtagat aaaagaccag aaaaaggaga aacagtatta ggtaatagtt tttttacatc
    17161 acctggagga aaaggggcta atcaagctat ctcagcttct aaactaggag caaatgtaaa
    17221 aatgatatca tgcatagggg aagatggctt aggagaggag ttaataagaa attttagaac
    17281 gaataaagtt gattatagct tagtatccag aaataatcac aaaagttctg gtgttgctgt
    17341 tataacatta tgtgaaaatg ataatagtat tgttgttgta ccagggacta atgagctagt
    17401 agatatagaa ttaattaaaa agaatgaaga agagataaag aatgcagata tagtattgct
    17461 acaattagag attccattaa aaacaataaa ttatgtagtg aatttctgtt ttgaaaatag
    17521 gattagggtt ttattaaatc cagcaccagc agtaaaacta aatgaagata taatagaaaa
    17581 agtaacttac ttaacaccaa atgaacatga atataagata gtttttgaca caaatgaagg
    17641 gatagaagaa gtattaaaaa aatatccaaa taagcttgta ataacagaag gaaaaaatgg
    17701 agctagattt tatgatggtg aggaaatcaa gcatgtatct tgtataagtg ttgatgttca
    17761 agataccaca ggagcaggag atacatttaa tggagcattg gcagtggcta taacagaagg
    17821 gaaaaattta tatacagcag tagaatatgc agtagtagta tcaggtcttt ctgtaactaa
    17881 actaggtgca caatctggta tgccctataa agaagatgtt gaaaaatatt taaataataa
    17941 ataaaaatct aatttgttca aatttaaaac tattaaaaat ggaggttaat tatgagagtg
    18001 agaaaaattt tatctttagg aattgctgga atactagcaa taggaatgtt aacagggtgt
    18061 tcaatggaag gaccaagtaa atcagataat aaaggtggtt cagacaagaa agatttaact
    18121 attggagtat ctacaataac tcttcaacat caatttttta tagatattga tgagggaata
    18181 aaagaaaaag ctaaagaatt aggtgtgaaa gttatagtaa atgacccaga ccaagatgta
    18241 gctaagcaaa catcagcaat agaagatttt atacaacaaa atgtagatgg aatgatagta
    18301 cttggaactg ataattcggc tatagtacca gcagtagaag gagcatttga aaaaatgcca
    18361 gtagttacag tagatgctgt attaaatact gaaaatatta caagttatgt aggaacagtt
    18421 agttatgatg ctggtaaaaa attaggggaa tacactaaaa aatacataga tgaaaagtta
    18481 ggtggaaagt cagaaatagc aatagtgaca gatttaaaat ctcaaataca aatgcagaga
    18541 atagatggat ttaaagatgc attaaaaggt tcagcaaatg ttaagatatt aaattcacaa
    18601 ccaggatatg acagagaaga atctcttaat acagtagaaa acttaattca atctaatcca
    18661 gatgtagata taatatatgc aacagctgaa aatagtgtac taggagctaa agctgcactt
    18721 gaatctgcaa aaaataagga tgttaagatt gtaggatttg acttaactga agaagcatca
    18781 agtggaataa cagatggaac tattcttgct atgattcaac aacaacctaa agagatggga
    18841 cgtttagctg tagaagcagt agtaaaagct ataaaagggg aaaaggtaga aaagaatata
    18901 ccagtaccag ctttacttta tgataaggaa aatataaaag actttaagaa ttagtaaaat
    18961 aaaatgagat tatttgaaag aaatcctatt taaaaaggag tgtcattatg agtaatataa
    19021 ttctgaagtt aagtaatatt gctaaagaat ttcctggtgt cagagcatta gataatgtta
    19081 attttgagtt atttcatggc gaagttcatg cactactggg tgaaaatggt gctggtaaat
    19141 caacaatgat aaagatttta actggggcac attcgaaaac ttctggaaaa tttatttttg
    19201 aaggcaaaga aatagagaat atatccccag atatctctaa aaagataggt ataaatgcca
    19261 tatatcaaga actaacagtt tttgacgaat taacagtaac acaaaatatt tttatgggaa
    19321 aagaaataaa tggcaaagtc ttaaccaatg acaaaaaaat gaatgaagag gctaaaaaga
    19381 ttttcgataa tatgggaata gatataaatc caaatagctt agtcaaagag cttagtatag
    19441 ctcaaaaaca aatggtagaa atagcaagag ttttatcatc agaaactaaa gtattaataa
    19501 tggatgaacc tacttcttca ataagtaaaa aagagactga aatacttttt agacttataa
    19561 atgatttaaa agagagtgga gttagtataa tatatatatc ccataggatg gaagaactct
    19621 ttgagatttg tgacagaata actataatgc gagatgggaa aacgatttcg accttgaata
    19681 ccaaagatgt atcgtctgag gaagaattag ttaatttaat gatagataga aagctagacc
    19741 aatttttccc caaaagaaaa gtagaaataa aagaagaaat tatgcgagtg gaaaatctaa
    19801 ctaagaacaa tgtttttaat gatattagct ttaatataag aaaaggtgaa atattgggaa
    19861 taggtggact agttggaagt aagagaagtg aaatagtaga agctatattt ggacttagaa
    19921 catatgattc tggaaaaata tatttaaaca atgaagaagt aaagtttaaa actccatcag
    19981 atgcaataga aaatggacta ggattaataa cagaagatag aaaaggtact ggattgtttt
    20041 tacaaatgag tgtcaaagaa aatacaacta tggctgggtt aaaaaagata tctaagttta
    20101 aatcaattat agatagaaaa aaggaaaaag agattttaga aaaatacata gaagctttaa
    20161 agataaaaac accacatatg aatcaagtta tacaaagttt aagtggtgga aaccaacaaa
    20221 aagctataat agcaagatgg cttttattac aaccagatat attgattatg gatgaaccaa
    20281 ccagaggtat agatgtaaat gctaaggcag agatatacaa tctaatggga gatttagtgg
    20341 agagtggtgt aagtataata atgatatctt ctgaaatacc agaattaata tcaatgagtg
    20401 acagaataat ggttatgaga gaaggtcata taagtggatt tttagaaggt gaagaaatgg
    20461 ttgaaaataa tgtattaaaa ttggctttcg gaggtaaaat taatgagttc aataactaag
    20521 cataaagaag aaaaatcttc tccattattg acaaaattaa caagtgtatt tagagaccaa
    20581 gggagtgcag ctgtaggact tgtaataatt tttataataa tgagtgtagc ttcatctaac
    20641 tttttaactt tagataattt aattaatgtt ggaagacaaa tatcaataaa tgcaatactt
    20701 gcagtaggta tgacatttgt aataattaca ggaggaatag atttaagtgt aggagcagta
    20761 attgctttag taggtacatt ttgggctaca acagtagtga attataatgc acctatatgg
    20821 gtaggtatga tacttgcatt agcaataggg acaattttag gagtcataaa aggtgcaatt
    20881 atttcaacac aaaaactacc tccattcatt gtaactttag ccatgttaac gataataagt
    20941 gggtcaagct ttgtatttac aggaggaaga cctatatcag taaatacaga tgcatttaag
    21001 atgctcggta gaggatatat tggaccaata ccaataccag ttataattat gattatagtt
    21061 gtaatagcag gacattttct attaaaaaga actaattttg gacgtcatgt acatgcagta
    21121 ggtggaaatg aagaggccgc tagattatgt ggagtaaaag taaataaagt tatagtaaaa
    21181 gtatacgcac tagctggact actaacagca ttagctggga taatactatc atcacgtttg
    21241 gcatcaggtt caccaactgt tggagatgga gcagaattgg atgctatagc agccgttgtg
    21301 cttggtggta ctaatatgat gggtggttct ggttctatag taggtacatg tataggtgtc
    21361 ggaataatag gaatattagg aaatggatta aacttattaa gtgtatcttc atataatcaa
    21421 atgattataa aaggtttggt aatgttattt gctatatgga taaataatat aaaattaaaa
    21481 aaatcagcta aatctaaata gtaattaaat tttagcttat aaactataaa aattattaag
    21541 tttttgtata tatttttata ctttaatttg catatatctt tatacattga atgggactat
    21601 gctaagtgaa aaagtttact atggcttaaa tagatgagat tagacttgaa attttaaatg
    21661 taaaaataaa tcttaggagg aaatatgaaa aaaattctct tagatacatt aaatagtaaa
    21721 aaacaagaat acatagatta tttaaaagaa cttgtaagta taaagactga agatgttggt
    21781 catggaatac taggtggatt tgaaaaagaa ggacaggaat atattgaaaa acttgcaaat
    21841 tacataggtt ttagtgtaga tagacaagaa atgagcgaag aattaattaa aaaagcaaaa
    21901 aatatatata aagaaggaaa tctaggtcac aattatcaag atagatataa tttaatttgt
    21961 aagtatagtg atgatttacc tggtaagaca attgtattta atggtcatgt agacactatg
    22021 ccaccaggtg atataagtaa gtggaaatat aatccatatc gtgctacaga agataatgga
    22081 aaactttatg gtcttggaac tgctgatatg aaatctggac ttatagcatc aatattagct
    22141 gtaaagctta taaaagatag tggtctaaat gtacctggta atgtaaaaat aatgtcagta
    22201 gtagatgaag aaggtggcgg aaatggtacc ataaatgcag ttatgaatgg tattggtgga
    22261 gattgttgca taatttgtga accatcagaa caaaacttaa tagttgcaca tatgggattt
    22321 gtattctttg aagttgaggt taaaggtgtg tctcttcact gtggtagtaa gtgggagggt
    22381 gtaaatgcaa ttgagaaggc tatgttgttg cttcaagata taaaagagtt ggaacataac
    22441 tggcttatga tatataagca tccactatta ccttcaccta caataaattt aggtgtaata
    22501 aatggtggaa cagcaggttc tactgtgcca gataagtgtg tatttaattt atgtgtacac
    22561 ttcttgccaa atataatgag ttatgaacaa gttgtaaatg atgtaacaaa tgtcataatg
    22621 acaagggcta atggagattt atggcttaaa gataataaac caaatataaa catatatcaa
    22681 tcagggttag gttttgagat gaacaaggat tctgattttg tagtgaatgc acataaaata
    22741 ctagaagaaa ctctagggaa aaaattagag ataaaaggtt caacagctgg caatgatgcg
    22801 agattaatga aaaatttagc tgaaatacca acacttatat tagggccagg ttcaatagaa
    22861 caatgtcatt caattgatga atatgttgaa attaaggagt atttagatag tatacttatg
    22921 tatgcaagtc taatattaaa cttataaaag taggaggaat atatatatga aaaaagtatt
    22981 aatagtaggg gaatcttggg taaaaaatat aactcatata aaaggatttg atacgtttgt
    23041 tacaactcat tatgaagaag ctgtaaagtg gttgaaagaa gcaatagaat ctggaggata
    23101 tgagacagtt catatgcctg cacatgtagc agcagatagt tttccgtata aattagaaga
    23161 gttaaatgag tatgactgta taatattatc tgatataggt tctaatacgt tcttattatc
    23221 taatagtact tttatagatt gcaatagtaa tccagataga cttgaactaa tcaaagagta
    23281 tgtaaataat ggtggggcgt taatcatggt aggtggatat atgtcattta caggaataga
    23341 tgcaaaagct agatttggag aaactgctat caaagatgta ttaccaataa caatgataga
    23401 taaagatgat agagttgaaa aaccagcagg aataattcca gaagttattg actctgaaca
    23461 tccagtatta aaaggtatac caacagaatg gccaaagttt ttaggatata acaaaacagt
    23521 agctagagat aattgtcctg ttttagcaac tattggagga gacccatttg tagctgttgg
    23581 cgagtttgga aaaggaaaat ctgctatctt tagttctgac tgtgcacctc actggggttc
    23641 aaaagaattt actgattgga aatactataa taaattatgg gttaatatgc ttgattggtt
    23701 aacatgctaa aaattttata aaacttgaaa acctcttact tgataaaatg aaacttacaa
    23761 gtcaagaggt tttattgtat aaaatggttg tatggtagaa aatattttta aataagttaa
    23821 aattagtcat tttggatgat aatcgataag ttattagaaa cagggaggtt tatataatta
    23881 atatggataa tttaaataaa aagtttttga tggaatatat agatgagata cagggagaat
    23941 taagagatat atcagataag atatggaaaa atccagaact tcagtataaa gaatactatg
    24001 cttcaaattt acaaaaagaa tgtttaaaaa aacatggttt tagtatagag ggattagaag
    24061 gtatggatac agcatttgtg gctagttatg gagaaggtag accagttata gcagttttgg
    24121 gagaatatga tgctttagat ggtttgtctc aaaaagtatc cactactaaa gaacctattg
    24181 tagatggtgg tgcaggtcat ggatgtggac ataacttact tggtacaggt tcaataggag
    24241 ctgtaattgc agtaaaggaa ttaatcaaac taaaaaagct gaaagggact ataaaatact
    24301 atggatgtcc agcagaggaa gatttatctg gaaaagtatt gatgataaaa aaaggttttt
    24361 ttgatggaat tgattgtgct tttagttggc atccatttga tataaatact cctataagaa
    24421 tcccaacact tgctaattac tctgtgaaat ttagatataa tgggattagt gctcatgcag
    24481 cacaagctcc ttataacgga agaagtgcac ttgatgcagt agaacttatg aatataggtt
    24541 gtaattattt gagagaacat atatttgata gttgtagagt acactatgta actactaatg
    24601 gtggaaaaat gccaaatata gttccagatt ttgcagaagt gtggtattac ataagaggag
    24661 ttaaaatgga gcatgtaaga gatgtatttg gtaggattgt tgatatagca aaaggagcag
    24721 cattaatgac tggtactagt atggaatatg atattataag tggagtatat gactatattc
    24781 caaatacaat attaacagat attctatcac aaaatatgaa attagtaggt gtgcaaaact
    24841 ataatgaaaa tgactactat tttgcagata aattagctga gacagtgtct atagataaaa
    24901 gaagtagtgt ttctagtgta ttaagtggaa ataaggatat tactaagatg aatttacatg
    24961 atgaggttac agatgataca tttactcata ataactgtat atcattatca ttggatattg
    25021 gagatgttag ctacataatt cctacagcac aatgctcttg ttcagtttgg cctattggta
    25081 tttctgccca tacatggcaa tcttgtgcca gtgcaggttc tgatatggga tttaaagcaa
    25141 tgttattggc atctaaatct atatcatgtt caataaatga tgtaatgctt gatgaattgg
    25201 ttataaataa agcaaaaaaa gaattgaagt atactgtagg tagttttgaa tatactccaa
    25261 ttatttgaag agtaatttaa tacaagtaag attgaattgt atgaattagg ataaatatat
    25321 aaattaacat tatttttaac ttaactttct aattattttc aatctaagat ataataatta
    25381 gaatatattt ttaaagtttc ctatttaagg aaaataattt gattttatgt tcgataaaag
    25441 gacttataat ttgtaagccc ttttattata tttatttttt gtttataatt aattttattt
    25501 tttaggttta gtcaggttat agattaccct gaagaaatgg tactgatact tgttagaatg
    25561 tcgttactcg gtcctttaag agcagttgga attagtaaag attcataaaa atagttttat
    25621 atatattaga ctttgaaaat atatgtttaa aaaccatttt ttactactac tgtttataaa
    25681 ttaggggaga tatcttaaaa tattagtaaa aggagtgcta agtgagtgta ataactatga
    25741 gataagaggt ttagttgata tagtcaaaac ttcaaaagaa tcaattagaa aagttaaaat
    25801 gttacaatta aatttaaagg agtaatatat gaaaaatgaa aatataagtt atttggcgtt
    25861 aggattatgt tttggtgtta ttttgggttc attatttaaa aacttagcta ttggtttatc
    25921 ctttggatta gttattggag tagtattaga cagtaagaaa aataaataaa acttctatag
    25981 taacatataa caattatact atttttgtat aaaatcattg gaaatcaaca atagaaaata
    26041 tttgttattt aaaaactaaa aatacagttt aaacgaccta aaaatatatt atataattat
    26101 tataacaaga taagtgagag taaggttatg ataatagagt aatatttttc aattattact
    26161 ctatttatat ttatttagtt atactatagt ttatattgtt attttaattt catatgcagt
    26221 atagggaatg gattaccttg ttcatcgaac tcagattttt taaagacttt aaatcccata
    26281 tgtttataaa agcctaatgc ttgttgattt tgctcattca catctacata atttacattt
    26341 aatacttcta ttgcatattc tgcaagtctt tttccaattc catttcctat actataatta
    26401 cctacaaata acatttcaat tttaaaatca tgtattccca taaaagcttt tataagacct
    26461 tttttgtctc ttacacataa aaaattacta acatatttag caccttcttt aacttgaggc
    26521 tttattgata ttattttttc ttcacttaaa aagtcatgtg ttgctcttac tgatgattcc
    26581 catgtatcta gtatttcttt catatcttta ttgttaataa tatttaactt ttcaaatttt
    26641 atcatttaat aattcctctt aagtaagtat gttacttaat attatatgtt aaatatgatt
    26701 taaaaaggtt ataagaatag ttttgtatta aatataatca aataaatata tttatttgtc
    26761 ataaattaaa tgtccactaa aatagaaaaa ataagaagaa ataggtaata aatgatagct
    26821 tatagcaaaa taaagatgta agtataacaa tagatgagat tgtaattaag taactatacc
    26881 taacagaaca ttcttagacg aatatagaag acttaaaaaa gaaaagagaa gtagataaga
    26941 atttattaat gattacatat aaaataaatg aattataatt ttattaatga tataataata
    27001 attaacaaaa aataaaagag gagcgagtta atgtttgatg gcatacactc attttctaac
    27061 caatgaaaca atagattcaa ttaaggttac taaagtctaa ttaacacata aacatatagg
    27121 ataaatacat ctttttaata tattaattat gttagactta caaaaaatgt aaatatatag
    27181 cttgttaaat cttgattgaa ccttaaataa taagggataa aaaatgactt gttcaaaggc
    27241 aaagaatata ctattaaatg atataagtaa cttagagata atatgtagta agtcaaaagg
    27301 actatatagc atcagtaagt aaaaagagta aaaacttaca taatgcacta taaaataaat
    27361 gagttagaaa ataaatataa tttatataat aaagtagtac aaaaaggagt atttatgttt
    27421 ggatatgtta aaataaacaa aatggattta acatttagag aatatgatta ttacaaagct
    27481 tactattgtg gactatgtaa atacttaaaa agaaatcatg gagaaatctc taggttttct
    27541 ttgaactatg atataacatt tttaattgtt ttattaacag cagtatataa cccagagtct
    27601 ataagtacag aggaagtgtg tatagtaaat ccatttaaaa agaaaaaggt aattacaaat
    27661 gatattacag aatatgcagc aagtatgaat attcttttaa cgtattataa attggaagat
    27721 aatttaatgg atgataaacg cataaaagat aaattggcat attatatata taaaaacaaa
    27781 ttaaaattag catatgaaaa gtatcctgaa aaagcagagt atataaaaca acaactaaat
    27841 gaattaaata agttagaaaa agataaaaat ataaatatag atgaagtatc tagtatattt
    27901 ggaaatataa tgggtgaagt atttgtctat aaaaaagatg aaaatgagag aaacttaaga
    27961 atgataggtt ttaatatagg taagtatata tatctactag atgcctatga agatttagat
    28021 gaagatttta agaaaggaag atataatcca tttatagagt atattgataa aaatgatgag
    28081 ttaaaagaaa aagtaaaaaa aataatagta acatcattag ggtttttagc taggggaatt
    28141 gataatctaa atctaaataa aaatgttgga ataatagaaa atataattta ttctggtgtt
    28201 tatttaagat atattaatat attagaaagt agaggtggaa agaatgtaca gtaatactta
    28261 taataatgaa aattttatga gagcaagagc attcatagaa aatagtgaat ttaaaaaagc
    28321 atatgatttt ttaaaaactt tgactgacaa atgtgcagag tggtattatt taactggctt
    28381 ttctgcaatg aacataggat attatgaaga aggtgaagac ttcttaaaaa gagcaaaatt
    28441 tatggagcct gaaaatagtg aatatagtga tgcacttaga agttatacac aatatagaaa
    28501 tgattatagc aatagggctg acaattataa tagaagaaga cgtaatgatt tagatggatg
    28561 ttgttgctgt tgctgtgatg attgctgttg ttgtctaggt gacgattgtt gtgaaaattg
    28621 tgctaagctt tggtgtttag attcatgttg tgaatgtttt ggtggagatt taataacttg
    28681 ttgttaggat gataaatatg agtataaaag ttgcttatgg aggagtttta ttagcattaa
    28741 atgtgatatt gttgactctt acaaatatta ttcctgtaaa tacattgttt attatggggt
    28801 tagcatcact tttagtatca attgtaataa tggaatgggg ctttaagagt ggaatcgctt
    28861 tttatatagg ttctatagtt ttaggattta tagtgatggc aagtaagtct caatggattg
    28921 tatattcact tacctttgga atatatggta ttgtaaaata tcttatagaa aaagataggt
    28981 ctatatatat tgagtatttt atgaaattag tatttgcaaa tataatgata cttatattgt
    29041 attttttatt aagaacaata gtttatattc caataaacat atttattata ggttcatttg
    29101 agatagcctt tatagtatat gactatgttt acagttcttt tataggttat tacaataata
    29161 ggttaagaaa gatgctattt aaaaaataat agttgatttt tatatttaat tttaatgaaa
    29221 ttgtagttta gataagtgta aatgatatat atgaatatat attaaagcat aaagctaaat
    29281 ttaatatatt cttagtgcaa catataaaag ctttgatttt tagtcaaagc ttttatatgt
    29341 tagatataaa tattagatat aatataagtt tttaaaatag atagtataaa ttaaatatcc
    29401 actatattta catctgtaga tagagaataa ataatatctt ttctttctac tattatctca
    29461 acatttttag aaatttcctc atcaagtgat atttgagata aaagttcctt tgttggattt
    29521 attgctattg gatttccaac tctttttagc atatttatat caccattagt atctccatag
    29581 gcatacgagt ttgataaatc taaatcatac tttacaacaa aatcgtttat agcattattc
    29641 ttacttatag aatcccacat tggaataact gtgcctgtaa atatattgtt ttcaaagaca
    29701 taatcacttc ctagaaaatc ggttacatta tattttttgg ccattttttc aacaagaaat
    29761 ccagggctac cagatataaa tataactatg tgtccttgat ttagatgcca ctcaatcctt
    29821 gaacgtgtgt atttataaac tctatctgct tttaatttaa ttacttggtc acttgtaaag
    29881 ttgatacagg ttttatctaa tccttttagt gaatcaacat aaaggtcaca tatttctaat
    29941 agataatcat cataattacc ttgtctttta tcccaatcca taaaagtgtc tctagcatgt
    30001 ttataccaat ttttttggtc aattatatcg tatttaatta attttttaaa atgttcaacc
    30061 ataagagagt ctctataaag agtaccatct atatcaaaaa aagctgctat atttttcata
    30121 agaccacctc aattaatcat ttccttatat tatattataa attgtatcta agttgttaaa
    30181 atatatgtat aataatttat atattaaaaa acaagtattt tagagtttaa aggatgtgac
    30241 tatatgaatt ttaattttgc aaaggctaca aattctagac ttatgggcag tttaggtctt
    30301 ataataaatt ggatagatga tgaaaacaat cacttttgtc aatatttttt attagatgca
    30361 gagggattag gattagcgga ttatgtgagt ttaaataacc caacacaaga agaagcttat
    30421 atggaagaag aaagattgat gggaggattt ggttcagata gagttgaatt aacgaaggat
    30481 gaatctttat ttttagtatc ttattttggt aataagaatc tttattatga taagctattg
    30541 ccaggagata aatgtgagta tattgatatt ataaaaaact ataaaacaga tttaactata
    30601 gaaaaattat acaataaaat atgtaaaaga gttgacgagg aagttgaatt tataaactat
    30661 atgaccatga gatttatagc atgggataga gaaagtttaa agtacttttc tggaagtgac
    30721 gagattgcaa atatgcacat tactaatata aatggaacat tacttaaaaa cgtggtaagt
    30781 gataaaggtc aaggcagata tataagtgaa gctttatatg aagataatga tggatattat
    30841 atatgtaaaa ttgctttttg cataagtaag tgttatgaaa ctggttttag aattaattct
    30901 ctattagtga cagataaaga atctatgtat gattttgaag tatttgatga gatttctaaa
    30961 agtgaatttg taagtgttta cagtgtaaat tcacctgaag aatttgcagg agtattttat
    31021 agagaaaatc catttctgtt aaaaagtaat atgaatgaag gtatattctt tactagattt
    31081 aattttaata atgaccatgt aaaagaaaat gtatatataa taaataatga tatgaaagct
    31141 atatattact taatgggaaa taagtttttt attggtactt ataatgaaaa tgattgcaat
    31201 tatatcaatg aaataagtca atctaattat agcgactata ttaaatttga agagaaattt
    31261 ttctttgagc aaaatgcatt atatgatttt gcagaatcag gttcattaga ttttgatgat
    31321 tttttggaat aataaattta gacattattt aagcgagatt aactgaattt aatctcgctt
    31381 aaatttattt ctataatatt gggggaatat tatgaggaat acatttatta ttgtggtcaa
    31441 ttattaaaat tttatacagt ttattataaa aaatttatat ttttctttat gttgtttgcc
    31501 aatattagtc tcaaatacga gtaccatact attctgaatc accaacacaa gagagcattt
    31561 ttaaatgaaa aagtcagata aaattaaatt ttatctgact ttttctatgt ttatcttatt
    31621 agattatgtt aatatctgtt gaccgaataa gcgcatattc agtcaacttt ctatacctat
    31681 aataatattg ggggaatatt atgaggaata catttattat tatggtcaat tattaatgtt
    31741 ttatacaact tacttaaaaa aatatatatt acatttcttc ctcatcaaat tcctcaatat
    31801 tagtagcttg tggtatacct tgtacagaca tattactaga accataagga gtaggatata
    31861 tattaggagg catagtacta ggcataacta caggcatacc ttgcattgta ttatgacata
    31921 taactcctgg tatcatcata agtacattag gaggcatatt ttgattacat gagaagttag
    31981 gaatacccat agtattgttc atattatacc ccatattact gttcatgtta ttaggcatac
    32041 ctgtattacc atacatattg ttattcatat tgccagacat attgttaggc atacccatat
    32101 tactattcat attattagac atattgttag gcatacccat attactattc atgttattag
    32161 gaacattgtt aggcatgccc atgttactat tcatattatt aggcatattg ttaggcatac
    32221 ccatattact attcatgtta ttagacatat tgttaggcat acccatatta ctattcatat
    32281 tgttagacat attattaggc atacccgtat tattattcat gttattaggc atattatttg
    32341 tagcagatgt attgtaattc atatttccgg atgtattatt tgtgttaggc aagttgtttg
    32401 gagaattact acttttagga taatgattta cattattagg atataggtta tttatgttct
    32461 ggttattagg tatttttgta taaccattag aaatttgagg gtcatttctc aattcatctt
    32521 ggggaatttc ttcattagta atataatctg catagttcgc tgggtctaag taaggcatgt
    32581 aactagtatc taaatccttt tttttcttgt tactcatatt aaacttgcac ctcctagaaa
    32641 actataaaaa agtttacaaa aacttattct aataatatat atattaaaaa agtttataat
    32701 atgtgcattc tatttatatt aaattaataa aaattattgt tgcaaattta gaaatgataa
    32761 catataatga acatatgaac agttattcat atattaaaat aatggaggat taaaaatgag
    32821 agaagagata aatgattgta attgtaatat agtacatgaa gaaatagtaa cagaggctaa
    32881 atcaactatg ccagatgaag aaatgttata tgatttagca gaattattta aagtatttgg
    32941 ggatactact agagtaaaga tattatatgc attatttgca aatgaaatgt gtgtatgtga
    33001 tatagcgagt ttattaaata tgactcattc tgcaatatct catcaactta gagtattaaa
    33061 gcaggctaga ttagtaaagt ttagaagaga aggaaaaaca gtgtattact cattagatga
    33121 tagtcatata agtcagatat ttgattgtgg attaaatcat attagagaga cttataaatg
    33181 ataaatgaga ggagtgaata tatatggaag caaataattc aattaaaaaa gaatttatac
    33241 taggaggatt aaattgtgct cactgtgcag aggagattaa taataaagtt tctaaactac
    33301 aagaagttaa atcttccaat ttaaatttta taaataaaaa acttacagta aatataaagg
    33361 aaagttttaa tgaggatact actatagaaa agataataga tataatagat tcaacagagc
    33421 caggtcttga tatacaaata agttctaaag aaaatgcagc tagtaaaaca tcaattaaaa
    33481 aagagcttat attaggagga ttaaactgtg ctcattgtgc agaagagatt aataacaaag
    33541 tttctaaatt aaaagaagtt gaatcttcta atttaaactt tgtaaataaa aaacttacag
    33601 taaatataag taataacttt gaagaggatg atgttataaa taaaataaaa gaaataataa
    33661 attcaacaga gccagggctt gatatacaag tagggtctac tgacaaagta aaagggagaa
    33721 ctactgaaaa atcaggagct gtaaatgata caaataagaa agaattaatt ccacttataa
    33781 ttggagcact tgtatatata tttggtatat atcaaacagc tacaggttat gaaagtcagt
    33841 ttagcaatat agtatttata gttgcctatg taatagtagg tggtgatgtg cttttaagag
    33901 caataagaaa tatatcaaaa ggaagagtat ttgatgagaa ctttttgatg gctctagcaa
    33961 cagtaggagc attagcaata ggggagttat cagaagcagt aggggttatg cttttctata
    34021 aagtaggaga gtatttacaa ggtgttgcag ttggtaaatc cagaaagtct ataacttcac
    34081 ttatgcaaat aagaccagac tatgcaaatc taaaagttaa ttctgaagtt aaggtggtgt
    34141 caccagaaga ggtaaatgta ggtgatataa tagtagtaaa acctggtgaa aaagtaccat
    34201 tagatggtgt agtagtagat ggagtttcta tgttagatac ttctgcactc acaggagagt
    34261 cagtgctgag agaagttgaa aaaggagatg aaatcttatc aggtgttata aataaaaatg
    34321 ctttattgag tattgaagtt acaaagagtt ttggagaatc aacagtttct aagattcttg
    34381 accttgttga aaattctagc attaaaaaat caaagacaga aaactttata tctaagtttt
    34441 caagatacta tactccaata gtagtaatag ctgctttatt aatagcattt gtgccaccac
    34501 ttgtaatctc aggtgaggta tttagtgatt ggttatatag aggattgata ttcttagtcg
    34561 tttcttgccc ttgtgcatta gttttatcaa taccacttag tttctttagt ggaattgggt
    34621 ttgcatctaa aaatggtatc cttattaaag gaagtaacta tttagaagct ttaagaagtg
    34681 tagatacagt agtatttgat aaaacaggaa cacttactaa aggtgtgttc aatgtaacta
    34741 agttaaatcc tgaaggtata tcagaagaag aattattaga atatgcagct attgcggagg
    34801 taaattcaaa tcatccaata gcaaagtcca tattaagtta ctataataaa aaaattgatt
    34861 tagatacaat agacagttat gaggaaatag cagcttatgg aattagagta aagcataatg
    34921 gcaattttat attagcaggt aatgagaaac ttatgaaaaa agaaaatata tcttattcat
    34981 cagctaaaga agtaggcaca gtagtctata ttgctgttga taaagtatat agaggttata
    35041 tagttatctc tgatgaaata aaagaagata gtaaaaatgc cataagaagc ttaaaagaaa
    35101 taggggtaaa agaagttgta atgttaactg gagataatga gaaagtagca aaaaatattg
    35161 ctcaagaatt agaattggat acagtttact caaatttact tcctaatgaa aaggtagata
    35221 gattagaaga tttatatgaa ggaagaactg aaaaagagaa aatagcgttt gtaggtgatg
    35281 gaataaatga tgctccagta ttagctcgtg ctgatgttgg tatagctatg ggaggcttag
    35341 gttcagatgc tgctattgaa gctgcagatg tagtgcttat gacagatgag cctagcaaga
    35401 tatctaaagc aattgaaata gcaaacaaga ctaataagat agtgtggcaa aacataatat
    35461 ttgctctagg agtaaaaatt atagttatga tactaggtgc tggaggagta gctactatgt
    35521 gggaagctat atttgctgat gtaggtgtgg cacttatagc agtagtaaat gctatgagag
    35581 ctatgagata agggataaat gtaaatccat actatttaaa aaacttataa ttatatattg
    35641 aagtatgtaa taatgaaagg tatcaataag tttcttttaa ctttattggt acctttttat
    35701 tggagcgctt tgcatatata aatttatact cattatccat tatgtttatt taatgtattt
    35761 atttagtgta gagtttttat attgtaattt taaaatttag acatagaagg tttattaata
    35821 agtttatatt aagtttaaat tagattcata aaaattataa tacttatgat tgtatatata
    35881 ttatattaat agttgtaatt tttacaacaa aatgtgtgtg ttaaaatact ctaaaccaac
    35941 agtatgactg ccattcaata aattatgtaa tataagaaag taaatatttt taaaaaataa
    36001 tttaaaaaag tattgacgaa ttatttttaa g
//
LOCUS       DAGSHT010000018        35262 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGSHT010000018 DAGSHT010000000
VERSION     DAGSHT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.019"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..633
                     /locus_tag="MJB72_000854"
     CDS             <1..633
                     /locus_tag="MJB72_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8261979.1"
                     /translation="QKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNSSTLYKY
                     FKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNAQAFYQV
                     FFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLVDTGYIP
                     AKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(790..1809)
                     /locus_tag="MJB72_000855"
     CDS             complement(790..1809)
                     /locus_tag="MJB72_000855"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBY8261980.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVSEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(1840..2454)
                     /gene="plsY"
                     /locus_tag="MJB72_000856"
     CDS             complement(1840..2454)
                     /gene="plsY"
                     /locus_tag="MJB72_000856"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBY8261981.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(2489..3808)
                     /gene="der"
                     /locus_tag="MJB72_000857"
     CDS             complement(2489..3808)
                     /gene="der"
                     /locus_tag="MJB72_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBY8261982.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(3842..5170)
                     /locus_tag="MJB72_000858"
     CDS             complement(3842..5170)
                     /locus_tag="MJB72_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBY8261983.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEVGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQINVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(5408..5608)
                     /locus_tag="MJB72_000859"
     CDS             complement(5408..5608)
                     /locus_tag="MJB72_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8261984.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(5610..6542)
                     /locus_tag="MJB72_000860"
     CDS             complement(5610..6542)
                     /locus_tag="MJB72_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBY8261985.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(6587..7519)
                     /locus_tag="MJB72_000861"
     CDS             complement(6587..7519)
                     /locus_tag="MJB72_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBY8261986.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(7574..8863)
                     /locus_tag="MJB72_000862"
     CDS             complement(7574..8863)
                     /locus_tag="MJB72_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8261987.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(8892..9584)
                     /locus_tag="MJB72_000863"
     CDS             complement(8892..9584)
                     /locus_tag="MJB72_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8261988.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(9599..10360)
                     /gene="pgeF"
                     /locus_tag="MJB72_000864"
     CDS             complement(9599..10360)
                     /gene="pgeF"
                     /locus_tag="MJB72_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBY8261989.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDLLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(10357..10818)
                     /gene="nrdR"
                     /locus_tag="MJB72_000865"
     CDS             complement(10357..10818)
                     /gene="nrdR"
                     /locus_tag="MJB72_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBY8261990.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(10840..11103)
                     /locus_tag="MJB72_000866"
     CDS             complement(10840..11103)
                     /locus_tag="MJB72_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBY8261991.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(11207..11980)
                     /gene="sigG"
                     /locus_tag="MJB72_000867"
     CDS             complement(11207..11980)
                     /gene="sigG"
                     /locus_tag="MJB72_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBY8261992.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(12072..12794)
                     /gene="sigE"
                     /locus_tag="MJB72_000868"
     CDS             complement(12072..12794)
                     /gene="sigE"
                     /locus_tag="MJB72_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBY8261993.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(12819..13691)
                     /locus_tag="MJB72_000869"
     CDS             complement(12819..13691)
                     /locus_tag="MJB72_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBY8261994.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(13981..15255)
                     /locus_tag="MJB72_000870"
     CDS             complement(13981..15255)
                     /locus_tag="MJB72_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY8261995.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(15539..16696)
                     /gene="ftsZ"
                     /locus_tag="MJB72_000871"
     CDS             complement(15539..16696)
                     /gene="ftsZ"
                     /locus_tag="MJB72_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBY8261996.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(16886..17239)
                     /locus_tag="MJB72_000872"
     CDS             complement(16886..17239)
                     /locus_tag="MJB72_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBY8261997.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(17338..18012)
                     /locus_tag="MJB72_000873"
     CDS             complement(17338..18012)
                     /locus_tag="MJB72_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY8261998.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(18017..18715)
                     /locus_tag="MJB72_000874"
     CDS             complement(18017..18715)
                     /locus_tag="MJB72_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY8261999.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(18738..19478)
                     /locus_tag="MJB72_000875"
     CDS             complement(18738..19478)
                     /locus_tag="MJB72_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBY8262000.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     KNVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(19748..20974)
                     /gene="murG"
                     /locus_tag="MJB72_000876"
     CDS             complement(19748..20974)
                     /gene="murG"
                     /locus_tag="MJB72_000876"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBY8262001.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSASKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(20992..22122)
                     /gene="spoVE"
                     /locus_tag="MJB72_000877"
     CDS             complement(20992..22122)
                     /gene="spoVE"
                     /locus_tag="MJB72_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBY8262002.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(22174..23529)
                     /locus_tag="MJB72_000878"
     CDS             complement(22174..23529)
                     /locus_tag="MJB72_000878"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBY8262003.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(23548..24516)
                     /locus_tag="MJB72_000879"
     CDS             complement(23548..24516)
                     /locus_tag="MJB72_000879"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBY8262004.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(24557..25930)
                     /locus_tag="MJB72_000880"
     CDS             complement(24557..25930)
                     /locus_tag="MJB72_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBY8262005.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHEYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(26097..28076)
                     /locus_tag="MJB72_000881"
     CDS             complement(26097..28076)
                     /locus_tag="MJB72_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBY8262006.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIEAFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(28088..28435)
                     /locus_tag="MJB72_000882"
     CDS             complement(28088..28435)
                     /locus_tag="MJB72_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262007.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(28916..29851)
                     /gene="rsmH"
                     /locus_tag="MJB72_000883"
     CDS             complement(28916..29851)
                     /gene="rsmH"
                     /locus_tag="MJB72_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBY8262008.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(29881..30627)
                     /locus_tag="MJB72_000884"
     CDS             complement(29881..30627)
                     /locus_tag="MJB72_000884"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBY8262009.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(31021..32307)
                     /locus_tag="MJB72_000885"
     CDS             complement(31021..32307)
                     /locus_tag="MJB72_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY8262010.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(32322..33587)
                     /locus_tag="MJB72_000886"
     CDS             complement(32322..33587)
                     /locus_tag="MJB72_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY8262011.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            33903..35000
                     /gene="ychF"
                     /locus_tag="MJB72_000887"
     CDS             33903..35000
                     /gene="ychF"
                     /locus_tag="MJB72_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBY8262012.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        10961 a         6481 c         3662 g        14158 t
ORIGIN      
        1 caaaagaaaa ggatattgat gtatttcata gaagctgctc aaaatattat ggaaaacgaa
       61 ggtatagaaa atattacact tcgtaaggtt gcagatatgg ctggctataa tagttcaact
      121 ctatataaat actttaaaaa tttagaccat ttaatatcat ttgcatctat aaaatatttt
      181 aaagattata acttgaatat ttcaagatgt atagaaaatg tcaatgatga atataagaaa
      241 tatataatta tgtggaaatt attttgtaag cattcttttg ataatgctca agctttttac
      301 caggtatttt ttaacttgtc tagtgatgaa cttagctata taacaaaaca atattatgat
      361 atgtttccag aagatttagg tattcatgat agtgacatat ctttaatgat tacaggagaa
      421 agtataaagg aaagaaataa gatactatta aacaaccttg tagatactgg atatatacca
      481 gctaaagatt tagacataat caatgatata attatttctt tttatcaaaa tctattattc
      541 ttaaaaaaga acacaggaaa aaattctgat gatgatgaat taactatgaa aatgatttct
      601 tctattgaat acataataaa acatgctaaa taaaattaaa atatggctgt ctcaaaatag
      661 agataagcct tttttattgt tgaaataata aaaatatatt taatttcata taaaaaagag
      721 tgcctcgtaa actaaaaagt tcattttgag acacccctaa aataaattta ttatttatta
      781 tacaatagtt tatatatcat cacctaaaac tacttcttcc atttcatgct ttttacttct
      841 catcattagt tctattccaa cttctttagg atttgaacct ttctttatta cagaatatat
      901 agcattagtt ataggcatat ctatatctaa tttttcagaa acatcatgtg ctacttcagt
      961 agcagttatg ccttctacaa ccatctttac ttcttttaaa gtatcttcta aactcatccc
     1021 tttacctatg agtataccag ctcttctatt tctactatgc atacttgtac aagtaactat
     1081 taaatctcca atacctgaaa gacctgcaaa ggtagacata ttagctccca tagcaattcc
     1141 aagtctgctc atttcactaa tacctcttgt cataagggca gcttttgcat tatcaccata
     1201 ccctaatccg tcacatatcc cagctccaaa tgctataata ttttttaatg ctcctccaag
     1261 ttctactcca actatgtctg gattcgtata aactctaagt ttagggctca taaataaatc
     1321 ttgtatcatc tgtgctattt ttaaatcttt agaagcaact actacggtag taggaatatc
     1381 ccttgctact tcttcagcat gagatggccc agatagtatt acatatggat tttgtggtaa
     1441 ttcttcttca cagacttgtg ataatctaag cccagtccct ttttcaagac ctttagctac
     1501 attaacaata acttgtcctt cttttataaa tggttttatt ttttggcaag tacttcttac
     1561 agcctgtgat ggaactgcta aaactattac cttacaatct tttatagttt tttctatatc
     1621 agtacttata gttatattat ttggaaataa tactcctggt aaatagtctg tattttcttt
     1681 agttctgttt atttttttag cttgttcctc ttttcgagtc cacatgctca catcattacc
     1741 tttttttgca agccctaaag ctaatgcact tccccagctt ccagtaccta atacacatac
     1801 tttctccatg tttaacacca cctgagtata atatatttac tattctttct tttgaccaat
     1861 ttttctctct gtcccattta ataatcgctt tatatttgct ctgtgattat atattactga
     1921 tgcagttaag aacagagcta ctattaaacc agctttattt tttaccataa tcataaatat
     1981 tggtgaacat cctattccta caacagaacc caatgaaaca tactttgtta tagctaccac
     2041 caatataaat actgctagac acattagtgt tattactgga tttacagcta gcattgcccc
     2101 tagtgaagtt gcaacacctt tgcctcctct aaatcctaaa accgctggcc aattatgacc
     2161 tgctacaaca catataactg ccagatatcc agctaaaaga gtatctattc caaccaatct
     2221 tgctgcaaat tctgatatta aaacagccat tacaccttta agaacatcac ctaaaaatgt
     2281 cattgctcct gctctttttc caagtgtcct aagtacattt gtcgaacctg cattaccaga
     2341 accttgagtc cttatatcaa ccccagcaat tcgctttgca actatgtatg aggtagatat
     2401 atttcctaaa agatatgcaa ctacagcaat aattatataa ctaaatatct ccactgaatt
     2461 tccccccttt agttatattt taagatattt attttttatt tttttgtcta tactcaaact
     2521 ttatggaagt tccttcaaat ccaaaatttt ctcttatctt attctcaagg tatctttgat
     2581 atgagaagtg agttaaatcc ttatcattta taaacaatgt aattttagga ggtctaatgt
     2641 cagtttgagt cccatagtaa atttttaatc ttctaccttt gtcagatggt ggttgattta
     2701 acattacagc ttctccaatt acttcattca gtgctgaagt tgatattctc ttagaatgtt
     2761 catttgacac atattcaact gtatctagta tcttgttcat tctttgatta gtttttgcag
     2821 aaacaaatac gattggagca tacatcataa atggaaattt ttcttttata tcttttgtgt
     2881 aattactcat agtcttatta tctttctcta ttaaatccca tttattgatt acaaatatgc
     2941 atccttttcc ttcatcatgt gctatacctg caacctttgt atcttgttct gttacacctt
     3001 ctagggcatc tattactatc aatactacat ctgctctatc tacagcactc atagctctta
     3061 ttacactgta tttttctatt gtctcatata ttttactttt tcttctaagc cctgctgtat
     3121 ctattagaag aaatttctgt ccattttttt caaaataagt atcaattgcg tctctagtag
     3181 ttcctgcaat tggacttaca attactcttt cttctccaag tatcttattg agtatagaac
     3241 tttttcctgc atttggttta cctgttatag ctactcttat tatatcttct tcatactctg
     3301 tatctaatcc tgctggaaaa ttttcaacta tctcatctaa taaatctcca aatcccatac
     3361 tattggctcc tgaaactgca aaaggcgttc caaatcccaa ctcataaaaa tcgtatatat
     3421 tatcaaattg actttgacta tcaatcttat taaccactaa gattactggt ttttttgtct
     3481 ttctaagcaa ttgtgcaatt tctttatcag cagctgttat tccagcttta ccatcaacta
     3541 taaaaagtat tacatgtgac atgtccattg caagcatggc ttgatttctc atttgagata
     3601 atattatatc ttcgctatca ggttcaattc ctcctgtatc cactaatgta aaatatttat
     3661 ctaaccattc tacttcagca aatattctat ctcttgtaac tccaggtgta ttttcaacta
     3721 ttgatatcct ttttcctgca aatttattaa atattgtaga cttaccaacg tttggtctcc
     3781 caactacagc tacaactggc ctacttatac tcattttatt tctccttcca tcttttgttt
     3841 tctattttaa aattttatcc acaaaatctt ttccttcatt caaacaaggg ataattttta
     3901 ttctcataag ttcttctaat tgatttaatt caatattatc taagaaaatt tcttcatctg
     3961 cttttagcat acttcttgtt atataaagag cttctcctaa ttctttatct tcaagttgtt
     4021 cttttaaatc cttagctgtt aaaagacctg atacagttat tgtctctcca aaaaacttgt
     4081 ttttgatttc atatacattt atttgaagat ttttaaactt gtccatcata gcatctgcca
     4141 tactctgtat aaattcatat gcagaatgac cagtagctat tgatactttc ttctctcttt
     4201 ttagaatctt ctcaggcaat atatttagat actcttcaat ctctgttttt aacttacgta
     4261 tcatacctac accatcttca aactgtaaaa aaccttcata ttcttcatat ccaggcaatt
     4321 cactattaga tagtatataa aattcatctg ataaaaatgc aaatcgagta cctaattttt
     4381 caagatattt ctgttgtatt tgatgtattt gctctatagt tttacttgcg ctcttatcat
     4441 taaatatatt taattctact agattttctc tatgctttgt aatcccaact ggaactatcg
     4501 caacactgtt tacatatgga taaagttgag ctagttcttt tattgttcta tccaattcct
     4561 ttccatcatt tatacctgga cataaaacaa tttgacagtt catttctata tgagcatctg
     4621 ccaatctctt cattatacca tataatttcc ctgcaaattt attgcttatc attttttgtc
     4681 ttaactctgg attagttgta tgcacagata tatttatagg acttattcta tattttataa
     4741 tattatttat atcttcttca ctcatatttg taagtgtgac aaaatttcct tgtaaaaaag
     4801 ataatctcga atcatcatct ttaaaataaa gagtttctct cataccttct ggcaattggt
     4861 ctataaaaca aaacatacac ttatttctac agcttttagc ttggtcaatt atcggatttg
     4921 taaattcaac acccaaatct tcatcatagt ctttttctat ctcgtatatg tagacttcgc
     4981 catctttttt ttgtatttct acatctaaat attcatcact tagtaagaat ctatactcta
     5041 ttatatcatg tattggctgt tcattcacag atataagtaa atctcctacc tctataccca
     5101 cttcttctgc aatactatct ttataaacct tacttataat attatttact ttttttacat
     5161 ttacttccaa tattttcacc actcttttgt attcatcaat gcttattata atgtatttta
     5221 atatgcaatt tatttattta ttatatttta tgatagcatt tttaagcatt tcatgtcata
     5281 ataacacatt ttaattctaa tagtcaatat aagtaaatag ctataaaata gattagaaca
     5341 aataatattc aaatttaagt ttcttaaaaa tttaaatatt atttgttctt aaaaaaattt
     5401 tataaattta atgttttact atgattaacg tcccatttgt catatcatga accattccac
     5461 ctacacactt tgacatcaac aatgccttat tttgtaagtc tttctcatca tttgcataaa
     5521 atattggaca acctccacca tttttcatat tcttatcaag tgtaactata gctagggtat
     5581 attcatttaa acctatatcc attcccatct tatgcacttc ctttctttcc tattttataa
     5641 ttttttagtg ataagttttt tcctttagag cttgaaagta ttgggcatga ctttacagct
     5701 tccattactt tttccacatt cttatctact ggtaaataag caatcattat actttcatcc
     5761 tctttatttc ttctaggtaa tggtacgaat gaaggttctc cataatctcc atataaacct
     5821 aaccttgaat atatattgta tactattgct tgtctttgac cggagtcaaa taatattcct
     5881 gcattagcat aatccttatt tttaggaatt atttgtattc ctacacctct attaatatat
     5941 acttctctat cgctttttag acctatattt gttatacctt ttaaatctcc cacctttagt
     6001 atagattcgt ccacaaaact aatttcagca ggcattacat cagctatgtc tccaatactt
     6061 tttctagtaa gaagtttttt taatataaag gctaacacta atccacaaat tgaacttatt
     6121 accaaactca tcccttcact tattttcgtt tctgcaagta ttacataata caatcctatt
     6181 gttaaaaaag atgtaactat acacatataa tttcttactt cataggttct agctatctct
     6241 tctatgaatg cagctcctct ctttacaagt tgaacttcct ctaaattttg caatgtattt
     6301 cttctatttg ctctcacttg tctaaattgt tctgctgcta gagataaaaa tgttatggat
     6361 gtataagaac gatttataag agctggaacc aataatgctc ctaatgaaga agcgacgaat
     6421 gctaatataa gttgagatag taaaacattt ggttgcgttg gatactgttt ttggtctaaa
     6481 ttcaacatta taattctaga tgttatacca ataataacag caacaaaaaa tactctatcc
     6541 attaaatcac tcctaaaata tttaatttga aattttacat gtaaatctac aaagtctttc
     6601 tagaaaaatc actctgcttt ccttttgatg tttctatgat aggtgtactt ttcactacat
     6661 ctatcatgac atctatatct ttgagtattg gcatatatgg tataaccaca gtactttttt
     6721 ctaaattggt cttagatata gcaacaatat caacctcatc tacatctcta tctatcccca
     6781 tatgaataaa tagattatgt attatagctt gtctttgccc tatatcattt actattccaa
     6841 atgcacctaa atctcttggt ataacttcta tagccagaca ttcatttcta tacttttctc
     6901 ttgtactttc tagacctatg ttagttatta ttacaccatt taccattaaa ataggccctt
     6961 caaaacttat ttttgcagga actatatctg ctatatctgc tatactgttc cttcttaaaa
     7021 atcttctaaa aatcaaacca acaatagctc ctgacacaat agctaagatt gtgcaataaa
     7081 aaaagctaag gccatacttt cttgcaaaaa cgatataaac tatcgaagat accagtgcag
     7141 aaaaaagact tatataactt ctagattcat acgtagatgc tatttcttca acgtaagcgt
     7201 ctccttttgg tactaattct tcattatcga tattctttag tgttattctt tcttgttctg
     7261 caagaccttg aaactgttgt attgctactg caaaaaatgt caaggctgca aactccttat
     7321 ctataagtgc aggtaatgct atagcaccta acgaagctga aagcccagat ataattatct
     7381 gttctaaata atcttgtggt cttgatgggt attgtttgtc tgtaactctt aaaactaatc
     7441 ctctacataa tattcctaca actaatgcaa ctataaaaga gtgtctaaat aaatttgcat
     7501 tcaataaact ttcttccatt ttctcagctc cttctcttat atagtttgtg caaaaaacaa
     7561 aaaaaattcc cttctattta aggaaatttt tttgtaatat tgtaactata aattcgctac
     7621 caatgccaag ttcacttttt acttttatac atccatttag tgatttgact atatgctttg
     7681 ttatagccaa tcctaaaccc gttcctccta catccctact tcttgcttta tctaccctat
     7741 aaaatcgttc aaatatgcgt tctacatctt cttttggtat ccctacacca ttgtctataa
     7801 ctttaattac gattttttct tcatctttta acactacaac ttgtacaatc ccattatctt
     7861 gggtatactt tatagcatta tcaactaggt ttaagaacac ttgctttata tagtctttat
     7921 tggatagtat acttattgat ttatcatcac atttatataa aacttctata tttttttcac
     7981 tagctgaatg ttgagttatt tcatatacct ctttaaatac atcatatact acaacatttt
     8041 ccataagaag agtatcttta ttttctacaa atgataacag caatatatca tcaatcaacc
     8101 tttttaatct atctgattcg ctttctatta tttctaagaa tctatttcta gtattcacat
     8161 ctatattttc atttgacttt agtgtttcaa caaacccact tatagatgta agtggtgttt
     8221 taagttcatg acttacattt gcaacaaaat cacttctcat attttcaagt ttaacttttt
     8281 tagtaatatc ttctatattt actatcgaac ctattattac gttgttagta ttctgaagat
     8341 aaactgggtc tacttttatc ttatatacga tatcatcttt agtagtaata ttagcagttt
     8401 cattatcttt agaaccaata aattgaagta tttgttttaa tatttcttct tcttttatta
     8461 caacattaat attatgacct tcaactaatt gatttggttc actctttatc atttcttttg
     8521 cttcatcatt tatcagaagt atatttccat ttacatctat agctaaaatt ccatgagata
     8581 tactctttaa tatagacctt agctgtaaat gtttatactg tacttcttct aaggttttat
     8641 ccatattctt tatcatatga ttaaaatttc tagctaattc gccaagttct cctttggctg
     8701 atatgttaag cctagcatga tattcattat tacttacttt ttttgagacg tatataaact
     8761 cttttaaata tttccttaat gttattgtgt atcttacagc taatataaca atggctatgg
     8821 acaacataac aataaaaaaa tatatactat ttaaatcttc cacaagataa cactcctaaa
     8881 atataacctt tttaatctat tttgtatcct acacctctta tagtctctat atatttttcc
     8941 gatttatctt catcttctat tttttttctt aaatatctta tatgaacatc tactgttcta
     9001 gtttctccat agtattcata cccccatatt ttatccaaaa gataatttct tgaaagtact
     9061 tttcctttat tttgaattaa taattttaac aattcaaatt ccttaagagt taactctatc
     9121 ttttcagagc ctttggtaac ttcatgtttt gataaatcta gttttaaatt acctgtagta
     9181 agtatattgt tttcttcacc taagctagtc aaattatatc tccttaaaac agaactaatc
     9241 ctagctaaca actcttttat actaaatggc ttagttatat agtcatcagc acctatttca
     9301 agcccttcaa ccttgtcatt ttccatattt ttagcagtaa gcattataac aggaatattt
     9361 tttaagtcct tatcacttct tatttttctc aaaacttcta ttccacttat atttggtaac
     9421 atccaatcta aaagtattaa atctggttta atttccttag cctttataaa tccgtcaaat
     9481 ccatcatatg aataactaac ttcgtaattt gacacctcca aattgaattt taatagctca
     9541 actatatgca tttcatcgtc tataacaagc actttagtat tcattaaaaa gctcctctct
     9601 attttatctg tagtaccgtt cctattcgtt ctgatgcctt attatgtttt ctataagagt
     9661 ggaatttatc tgctctacaa ctagtgcata aatttaaatt tattatattt tcttttttaa
     9721 ctccactaca tctaagcata tactcattaa ttttccataa atctaaatag taacttccct
     9781 cttttattat atagaacttt tcatcattat ttgtaagaat tgtgttaaat ttttctacta
     9841 aatcttcaga cacttcataa caacatggtc caattgatgg cccaattata catactaaat
     9901 cttcaggaac tgttttatag cattcagaca ttaattttat tgttttatga cctatattac
     9961 taaaagtccc tctccaacct gcatgagcca gtcctatggc tttattttta gcatctataa
    10021 ttgatatagg tacacagtct gctgtaaaca gtaaaagtgg tactttttcc aagtttgtta
    10081 taagtgcatc tccatctctt cttttcccta tagtatcttt attgactata ttaactatat
    10141 ctgaatgtat ttgagaatta ctagttagat tttcaaatgt caaatcacat tctttacata
    10201 cttttaacag atcttctcta ttttttgcat ctatattctt acatgttata actagattta
    10261 cagaatttaa tttttcatca agttcttcac aattaccact taaattaaca tactctttaa
    10321 tatcttgtac attttttata tttatataat ctttcatcat ttgtctcctt tttctatcag
    10381 tatactttta agttcattta caaaagtatt tatatcttta aattgtctat acacagaagc
    10441 aaatcttaca taggcaacct catctaactc tttcaattta tccattacca tctcaccgat
    10501 ttttttggtt tcaatttctt ctataaaaca tttatttatt tcattttcaa tatgtacaac
    10561 aatactttca atctcgtcaa tagaaacagg tcttttttca catgctttta gcaatccata
    10621 ttttatcttt tctctatcaa aatattctct actattatcc tttttaatga ccattaaagg
    10681 agttgtttct attttctcat acgtagtaaa cctcttttta catgattcac actcacgtct
    10741 tcttctgata gattttaaat ctgtatgtct agaatctatt acctttgatt ctttataatt
    10801 acaataagga cattgcatct atattcctcc tttgtttaac tatatctcaa actcatctaa
    10861 gcttaattct gaacctatat ttactattat tgtatcacaa cctattttaa gtatatcatt
    10921 ccaaaatata acttcttcat cccctcctct tgaaaaaaaa cttctacttc catctccaaa
    10981 aatggtaaat gagactacct taccttcgtg aatatccatg tctatgtcta ttataaaccc
    11041 catccttttt ccatttttta catttataat ttctttttcc attatatcag aaactctaat
    11101 catattgtac tcaccctttc tttaaaaata aatatgtatc gaagtaaaaa aaaatgacct
    11161 atataggtca tttaaattaa aataatgttt acagtccatg gattacttat acatattttc
    11221 tcatattttt taaagcattt ttttcaattc ttgatacttg tgcttgagat ataccgattt
    11281 catcagcaac ttctatctga gttcttcctt tgtaaaacct taaatctaat accaactttt
    11341 ctctactatt taatttctta atagcttctt taagtgatat ttcttgcaac caattttcat
    11401 cagtatcttt tttatcttga acctgatcca taacgaatat tgcatctcca ttgtcttgat
    11461 atactgggtc aaacagagat attgggtctt gtatggcatc aagtgccatt acaactgatt
    11521 ctacttctaa ttctaattcc tttgctattt ctgatactgt aggttcttta gaatttgttc
    11581 taattaatct ttctctaact tgtagtgcct tatatgctat atcttttaat gacctactta
    11641 ctctaattgg gttattgtct cttagatacc ttctaatttc tcctataatc ataggaacag
    11701 cataagttga aaaccttaca ttttggctta aatcaaagtt atctatcgct tttataagac
    11761 caatacaacc tatttgaaat aaatcgtcta tattttctcc tctgttgtta aactttttta
    11821 ttacactcaa gactaatctt aaattccctc ttacaaactg ttgtctggct tcttcatcac
    11881 cattttttat ttgtaataaa agttccttca tttgcttatt tttaagaact ggaagttctg
    11941 atgtattaac accacatatt tcaaccttat taacttgcat atattttcag tcctctctca
    12001 aaagtattca tctattgaaa ttatttacat ttccaatttt tttatacttt ttaagaagaa
    12061 ttaatttagc attatacaaa ttttttcatt tctttttgca accttgagat tattttcttt
    12121 tcaagtctag atatgtagga ctgtgatatt ccaagcattc cagcaacttc tttttgtgtt
    12181 ttttcaatac ctttatcaat aagaccaaat cttaattcca taatttgctt ttctctgtca
    12241 cttaatctgt caagtgccat aaccaacaaa tctctatcta tttcttcttc aattattttg
    12301 tatatttcat cattttctgt acccaataca tcagataata aaagttcgtt tccatctaaa
    12361 tctatattta aaggttcatc aaatgatact tctgttttct ttttattatt ttttcttaag
    12421 tacattaata tttcattttc tatacatctc gaagcataag ttgccaattt tatattttta
    12481 ttcagtttaa aagtattaac tgcttttatc aaacctatag ttcctattga aatcaaatct
    12541 tctacatcta tacctgtatt ttcaaatttt ctcgatatgt agactactaa tctaagattt
    12601 ctttctatta aaattgactt tacactttca tctgtttcta gcttttgtaa aagctccatt
    12661 tcttcttctg gtttcaatgg aggaggaagt atatttgccc ctcccatata gtatattccc
    12721 tttggcttta ttatttttaa tttaatacct aacatagtaa taaagcttat tattctttct
    12781 ttcaatcgta acattccttg tcccccttaa attttcacct aaaatccagg caatatattt
    12841 ggattcatta ttgctccata ttctctatca ttaaaatttg aaataccaag tattatattt
    12901 cctatctttt gctcatctac ttctatatag tcagctttta agcctattat tatgcttgtc
    12961 ttattacttc cagcatgttt ataaggtatt attctaactc tacctgaagt tttatcatct
    13021 aaacttccta taatttcttg tgctttcttc atatctatat cttcataatt aatatttaat
    13081 agtttttctg gaataaatcc ttctaacaca ctagacttaa ctattattac atcactttga
    13141 cttattgggt cttttaacaa attaccacta tctactaaag ctttacagca aaaacttttt
    13201 tctagtaagt ttatatttat agtctttgta agctccttaa tgtacttaag cattttcaaa
    13261 tctttataaa tgtattttaa taattcacaa ctcacataag tacatgcaat taagaaagat
    13321 atttttaagt gacttattcc tgtaaaataa attataaaat aagtgcttcc agaaataaaa
    13381 atgtttacta gataaaaaac taacgctaca tgaacataat cttttttgct cttacttcta
    13441 aatgaaatta aagttattat actcatgatt attattttaa atggtacagt aaatagtata
    13501 ttcattgatg gatacaaata agcaacagaa taggcacctc ctaaactagc ccccaataac
    13561 ttattttttt taggattata tcttttagta agaatagaag tacaacttat aataatataa
    13621 tttattacta ggttttctat aacatagtac tcaatataca ccatgtatcc ccccttaaaa
    13681 cctttaaaca tattataaga tagtatgtac aaaattttat gtcacttttg atggtcgaat
    13741 ccaaaacaaa ttctatttaa aaagaacaat gatatatgtc ttatacttta tgtcgtcaaa
    13801 agatattttt ttaatgccaa acaaatagac ttttttgtag aatccaatat atctaggtag
    13861 aagttattcg ctctactttt ggtatattta caaaaaaaga ctacataatt taataatata
    13921 tcaagtaaat cttttactta tataaaatca aattatatag tctgtaaatt caaatataaa
    13981 ttattgtgct agtaatccat ttgcatattt tgcagattca tttagtgctt gttctggagt
    14041 tatgtctcca gctatcatca tttgaagttg tttccataaa tattcatata tctctactcc
    14101 atgaggtcca actgtcaatg gtctatacat atgtttatct cttgtcacca tttcttcaaa
    14161 tcttgggtct ccttggtatt tttcaccttt agcaactgga gctctaggta tttctttgtg
    14221 gaaagcttcc attgattctt tgcttagcat atactcaaga agtttaaatc caagttcttt
    14281 atttttacat gctgacatta atgttaaatc atctactgaa gcaaaacttc caactgtctt
    14341 atcttctaat gatgttacat atccccagtt taaatccggg aatgatttat caaaagtact
    14401 tgtagcagca gaggatagca ttatagtaaa tgcagctttt cctggtccaa atacactttc
    14461 aatcatttca ttactatctt ttgataaagc atcttctgga atatactttt tcatatcatt
    14521 tataaagttt gcagctttca agccttctgc attattaaat ttaactgatt ttaaatcatc
    14581 attatatata tctccaccag cttgccataa atacggatac cagttccagt ttaagcttcc
    14641 aaatacttta gaaccccaac cttgtgcaag accccactgg tcgatttttc catctccatc
    14701 agtatctttt gttgcttttt cacagattct tttaaaatca tcccatgttt ttggaggttt
    14761 ttctcctaat ttttctagta tatctttatt gtagtacaat acgcctggat tagcagcttc
    14821 tataggtaaa ccataaatac cacccatcat ttttgcatca tctaagtata gataattatc
    14881 tgaagttcct gatttttcta aataaggtgt taaatcttct accgcaccca tttctataaa
    14941 ttgaggaaac atctctgcat acatgtatcc tatatctgga ccttcacctg cacttattgc
    15001 tgttgcatat ttttctgcgt agttatccca tggtactatc tgaaattcaa tttcacagtt
    15061 attttctttt tcaaattttt caaatatcgg cttccatgca tctgcatcat ggtctgttaa
    15121 tggtgcagac catacaacta atttttcttt accctctttg cctttagaat cttctttttt
    15181 tgaacatcct gcaagaattg atactgatag aatccctacc ataactaaag aaagaacctt
    15241 ttttagttta atcatgctta atcccctttc agactttaat ttgtcttttt cctcaaatgt
    15301 ttacatttaa ttcagattta atattaacaa ataagattac tcttgtctaa tcataaaaaa
    15361 ctatactaac tcctataact atattttttt tctataccta ttagatttaa ttcatactaa
    15421 aaacttactt tttaaatttt atcaatattt aaaaatattt ttaagtctag ccacaaacta
    15481 actatctaaa ttgaacaaaa taaaaagaag tctattgact tctttataag cttacatatt
    15541 atcttcttct tcttaggaat gtaggtatct ccatgctctc atcatcatct tcttcaatta
    15601 tacttgcctt tttaggaggc tctatttttt cctcagcaat tacttcttct tcaatttctt
    15661 ttacagcttg ttttactgtt gtatttaatg aagaacgtat gcttgattta ggtttagtat
    15721 ctaaatctaa ggcaccattt tgtaatcctt caaatcctgt cgcgattact gtaatcatta
    15781 tttcatcacc taaatcttct tttatagaag ctccaaatat aacatttgct tctgggtcac
    15841 aggattccat aactaaagtt gatgcctcat taacttcaaa taatcctaag ttaggaccac
    15901 ctgtaacatt aagaagtact cctttagctc cttgtattga tgtttctaga agtggagatt
    15961 gaatagcttg tcttgcagca tcaattgctc tagtttcacc actagcacta cctataccca
    16021 tatgagcaag tcccttgtct ttcattatag tagtaacatc tgcaaagtcc aagtttatta
    16081 acccttcaac tgctataagg tctgaaattg attgtatacc ttgttttaat acatcatctg
    16141 caactgcaaa agcatctaac attgatgtat ttttttgtac tatctgtaaa agtctatcat
    16201 ttggtatcgt tataagagta tctacttttg atttaagttc tgctattcca ccttcagcgt
    16261 tcttcattct tatcttacct tcaaatgcaa aaggtttggt aactacacct actgtaagta
    16321 tccccatttc ttttgccaag cctgcaacca ctggagcagc tcctgtacca gtaccaccgc
    16381 ccattccggc tgttacaaag accatatcag ctccttgaag taactttact atttcatctt
    16441 tactctcctc agcagctctt ttccctactt ctggatttgc tcctgctcca agtcctctag
    16501 ttaatttttc tcctatttga actttatatt cagcttttga tgtatataaa gcttgtttat
    16561 ctgtatttac tgatataaat tcaacgcctt ttagttgagc ttctaccatt ctattgacag
    16621 cattgtttcc accgccacct acacctatta ctttaatttg tgcacattct tctaattcta
    16681 cgtcaaagtt tagcatcata aacccccttc tctcatgatt caaagttatc gtatgtatta
    16741 attatatcat tttcttcttt tattttgtta acctttaagt aacatattcc acaaaaacgt
    16801 aaattttcct ttttaaattt cttacttata ttgtcaaata ttactcttta agtataaaat
    16861 aatttacgta tatattaaat gctctttaat ttttacgttt tttgttcata atattactaa
    16921 taatatatcg tctaattata gataagttat taaacaatct cataccaaaa actaatacag
    16981 caacatagta aagagaaact cctaactttt ctcctatata agtcaataat atagccaata
    17041 tagcattacc tatgaaacca gtaataaaaa ttacattatc atagttatct tcaaaattgg
    17101 attttatcgc tccaaaaata gaatccatta ttgcaagaat agctactgac atatatatgg
    17161 aataatccat agaatatgta aatggaatat agtaacctac tactacacca aatgctaatc
    17221 ctcctaaaac ccataacata aatcttcacc tctttatttt actttgaaac acaactacat
    17281 tacaatcctt tttattaatt ttttttactt tttctttatt tttttgtatt tttcgtttta
    17341 ttttgcaaat aaattcactt tatttccatt attttcctga acttttatat taattccata
    17401 tatatcctta agagatttag catatgattc tggagagttt atagctgcac tcaataaatc
    17461 tatatctcct atagctttta ttataaatgg ttgtccatat gtagtatcat ttactgtaat
    17521 agtagctcct gaacatttta tcttacttgt tgctagtact ctttctccat ttatagaaat
    17581 tgctcttgct ccagcctttt ttaaatcatt taaaaccctc aaaatatcta tatcatgtat
    17641 aatcaaatcg tttggattct gtccgtcttt taactctctc tcactatccc tcatagtaat
    17701 agttatgcct ggccctgtaa cagttgaata accacttaac tctttaactt cttcaagctc
    17761 tttttgcata agtgtttgga tagattcatt tccattcttt tttgcttctt tatattcatt
    17821 caaagttttt ttgttctttt taagagtttc ttttaaattt tttatttctt tttttgtatt
    17881 ttctacctca ctttcaatac tttcagtttg acctaatgtt atgtatactc tatttgggtc
    17941 tagtgatttt atataagttg atatgaaaac gcctaatatg aatgctccta atataactat
    18001 tttctttttc atactattat ttcccctctg ctttgtttat atcactatga ccatttattt
    18061 ctatattgtc ttctttggtc acttttactt tggcattata atatgtttct atttcccaga
    18121 caataccata ctttatatta agtgccgaag ccaatgtatc agaatcacca attgctttta
    18181 ttataattgg ttcttttatg acagtgtcat tcatataaag cttgtcttct tttaaataca
    18241 tatatgtatt agaaactatt ctctggtcgt ttatagatat ggcacttgct tgtgctgaat
    18301 ttaatttatt tatcattgat aataataaat cataattata tagtatacta tcatttttat
    18361 cagagtttcc taagtctttt gtagaattta tctcaatttt tatgcctgtt ccttcaacat
    18421 ctgtatatcc agctaatgtt tcatattttt ctatttctga cttaagtatg tcttgttctc
    18481 cttcattgtt tttgcctcta taagtctcta tatctttctt cacactatta acttctgatt
    18541 gtagattctc tttttctttt tttaaagatt taagttcttc tgtaagttgc tctcctcttt
    18601 taaaagttgt catccctcta ttttctatat taataatttt aagttgtaaa ctaacaagaa
    18661 cacccaatac aatacttcct atgattatac ttttatttat aaatcttttt ttcaatatat
    18721 cacttcctta caattgtcta tccttctgga ctatacactg ccaactttcc ataattaaga
    18781 tttatagtcc ctccattagt atttttattt tgcaggtcaa tcagaatttt acttacccta
    18841 gatatattat aatctaagtt atcatcatta gaaagtaaga tttttatatt acttcttgta
    18901 agcatttcta tattgctttc tttttttaag tttacataat ttatttttct ataaattcct
    18961 tcttctttta aataatttaa agttttaaat acattcttct tagttttata atttttaaat
    19021 tttatagatt tattatcatc aatgctataa tctacatcta ctatcaagtc ttttttactt
    19081 tcattagaac ctcttagttc ttccaataaa tttccttttt tatctatata acaataatta
    19141 tcttcatctt ttaatactgc aaatatttct ttttctttta aacttataat tatcttattg
    19201 ggaagttttc tttttatttc cacattttct atatatggat tttttataag tttatttttc
    19261 atatctttaa aattgtatgc aaaaatattt tcatttagat ttacatttag ttctttcatt
    19321 atatttgatt tggtcaccct cttatttcca attacatcta ttttctttac atcaaataaa
    19381 tcacttttta caattataca tatacaaaaa gataacataa gtataaaaac taaaactatc
    19441 atgacattat tcgtatttaa ctttcttctt tttttcaaat ataatcaacc cctttggtta
    19501 ataaatacac ctttagagct tgtttttcct acaaaaaaat tataatattt aaagtcaaat
    19561 ttttgcattt tattacataa catccaaata aggtattaaa ttttataaat aaatcaatat
    19621 ttcatcaaaa taaacatagc atactacata ctatttatat atttaaatct gtatacatac
    19681 aaataaaccc ctaatttata ataatttaga aaatctaata tattataaat taggggtaaa
    19741 tatttactta tcttttcttt attcctatta ccttagcttg accttctata cttggagtcg
    19801 tttctttttt tatttctttt acttccttaa taactttttc tttttttgtt tttttagaag
    19861 ctgatttttg tgttgaattg tatactttca ttatctcatc atatataagg tctatggctt
    19921 caggcttacc tatagtttta cttgcatttg ccatatcaac caaaagttct ctatctccta
    19981 aaagtttaaa tactgctgta tttaggctct ctggagttag atttttttct aaaatagcaa
    20041 ttcctgcccc ttgtttttct atactcttgg cattatactc ttgatgattt tcagcagtat
    20101 aagctttagg tattattata gatggctttc caagagccgt tatctcagct aatgaaattg
    20161 ctcctgcgct acctataacc aaatcacttg ctgcaagtgc atttgccata tcttctaagt
    20221 aaggcacaac tttttggtat ggtttaagat ttatatcgct aatacttccc ataaattcat
    20281 cataatagct tttgcctgta gcaaatataa aagctatatc ttcatttacc atattcttta
    20341 ttacaagtct cattgcatca tttatttttc ttgaacctcc actaccacca tagcaaagta
    20401 ccattctttt ttcgtcactt attgatagat tctttcttgc aatatttttt cttgataata
    20461 aaatttcttt tctaactgga tttcctgtaa aaactaattt atcttctgct gcctctggaa
    20521 atcttttatg cgaatcttca aaacttgtaa gtacctttgt aactgtttta gataaaatct
    20581 tattagttac accaggaaaa gaattttgct catgtattat ggctggtatt ttccccatag
    20641 atgcattaaa taaaacaggt ccactaacat atccaccagt tcctataact atatctggtt
    20701 taaatttctt aacaatcttt cttgattgct ccaaaccttt aaaaagctta aaaactcttt
    20761 ttacattatc gaaatctatt tttcgcttaa aaccttgaac tgttacagtc tttaattcaa
    20821 atccatactt aggaactatt tctgactcta ttcccttttc cgtcccaaca aaaattatct
    20881 cagcgtctgg atgctcatct cttattttgt ttgcaatagc tatagctgga tacacgtgcc
    20941 ctccagttcc accgcctgat aataaaactt tcatcaaatc atcatctcct gttaattaat
    21001 tttgacatgc tttgagatgt ttaacactat tcctacagct cccataaaga tggtgagtga
    21061 agttcctcca taacttataa atggtagtgc tacacctgta gctggcattg atgatgtcgc
    21121 aactgctata tttaaagctg cttgtattcc aatttgtgca cctataccta taacaaccat
    21181 acaagcaaat acattagaag tcttcaaagc tattcttaca catctgtaga ctaaaactac
    21241 aaatagcata ataactatta tacaaccaat caatcctaac tcttctccta ttatagcaaa
    21301 tataaagtca ttttgtggct ctggtatata aaaatacttt tgtttactct ttcctaagcc
    21361 tagaccaaac aatccacctg aacctagtgc atatagacct tgtattacct gatacccctt
    21421 accaagaggg tcttgaaaag ggtctaaaaa tgatgtaaca cgactaagtc tatagggttc
    21481 agcaattact aatgcagcaa ataaagcagc cccactccct atcatagcaa atacaatttt
    21541 catatccatt ccagcaacaa atatcattac aaaagtaact agaattacag tacctgctgt
    21601 tgacaaattt ggctgaagta ttatcaacgc aaaaaatatc cctggaacta cgagaagtgg
    21661 tacaacacct tttgtcaaag attttatttt atcataattt ttttcaatta attttgcagt
    21721 taatattatt gtagcaaact ttgcgatttc tgcaggttga aatgttaaag ctcctattcc
    21781 caaccatctt tttgctccgt ttgcttctat ccctaaaggg gtcaaaacta acaataataa
    21841 tactactgct atagctccaa ttgctgtagc atttttcttc caaaaactat aatctattct
    21901 tgatgtaata atcataacta taaatccaag tacagcatat attacattct tttttagaaa
    21961 atagtacgca tcattgtgtt taaatgaaga ttgtataaaa cttgcactaa acaccataac
    22021 tattcctaca aatactaaaa gcatagtagt gtaaaacact actccatcaa attcagtctt
    22081 catccttatg tcaatttgtt ttttcaaatt ttctttaggc atccaactaa tctacctcca
    22141 ttcttccatt taagtaagtt tagaggtaag agattatttt aaattgttta cattatcttt
    22201 gaagtctttt cctcttactt caaaactttc atacatatcc caacttgcac aagctggtga
    22261 aagaagcact atatctccac tttttgaaat ttcataagaa gtcttaactg cttcttccat
    22321 atcttttact atatgtatgt cattaaatcc tttattttta gcacaatttt ctatatttga
    22381 tgcagtttct cctaataaga ccaatgattt aacatatgac tttgcagttt caagaagttc
    22441 atcaaaatta cttcccttat ccatcccccc tgctataagt attataggtc tatcataaga
    22501 ctgtatagct tttatagtgg aatctggatt agttcccttt gaatcattta catatattac
    22561 attgtctaaa tttcttacaa actcttgtct gtgttccact cctttaaatg tcataagtac
    22621 atcctttata acttctaaat caattttgca gacatatgct attgctatag ctgccataca
    22681 attttctaag ttatgacctc caggtaaact tacatcatct ttatttagaa atttgatttt
    22741 atcatctata tctattatta tgtcattatt ttctaaatat actcctccat ttactttttt
    22801 agttcttgaa aagtaaatta ctttggcatt gcatttatca gctaaagatt ttactatctc
    22861 atcatcatag tttaatacac agaaatcctc aacagtttgg ttcataaata tatttgcctt
    22921 tgcttctatg tatttttcca tagtatggtg tctatttaag tggtcctctg taatatttaa
    22981 tattgcgctt actttaggtc taaattcatc tatactctca agctgaaaac tgcttagttc
    23041 agttactaac acagactctt cacttgaagt ttctatagta tctattacag gatttcctat
    23101 attaccaact acatatgtat ctcgttttgc tcttgaaaaa atttctccta ccaaactagt
    23161 tgtagttgtc tttccatttg tacctgtaat ccctataaaa attggattat tcgatagttt
    23221 aaaagctaat tctacctcac caattatata cttgcctgaa ttttttaatt tcaatatgaa
    23281 tggtaggtct agtggtacac ctggagacac tacaaccata tctatatcat ctacatcttc
    23341 tgggtgatgt cctaatatgt attttatgtc atttatagat ttcaattcat caagtatatt
    23401 tctcaattta ttttcatctt ttatatcatt tacgattata cttgcaccca atttatctaa
    23461 atgtttaatt gtagatatac cagtttttgc aagtcctact aataaaactt tttttccttt
    23521 taagtccatt tttcattccc cctagcccta aaatactgct attatactta tccaagctaa
    23581 aacaactgtt ataatccaaa aaataaatac aactcttgtt tctggccaac cacactgttc
    23641 aaagtggtgg tgtattggag ccatcttaaa gaatctcttt cttgttgcct taaaatatcc
    23701 aacttgaatc aaaactgata gagcctccgc aaaataaatt cctccaatta taggtattat
    23761 aagcactgag ttagttagaa ctgaaaatgc tacaactgca cctcctaatg ccattgaacc
    23821 tgtgtcgccc ataaacactc tagccggata tgaattaaat cctaagaaac ctaaacatgc
    23881 tccaacagtt gctgctgcta aaactgcgac ttctgtattt ccagcaatag aacttgcaaa
    23941 tagcataaag aatacagaaa ctattaatgt tactcctgat gctaatccat ctaatccatc
    24001 agtcaagttt acagcattta ctattgctac gattataaac atcattattg gtatatataa
    24061 tattccaaca tttatcacaa aatctgtaaa tggtatcata agttgtgaag cacttgatga
    24121 tgatgtatac tgataaaaag caacataaaa agataatgca acttgaagga ttattttttg
    24181 atatgctttt agtccaagtg atctttttag ctttattttt ataaaatcat ctaaaaagcc
    24241 aataaaacca aaacctgcaa tacatataag acctactgcc atatcatgac taacttttac
    24301 tcttgtaagt ccagttatta gaattgcaac aatcattata attccaccca tagtaggtgt
    24361 tccgttttta gctagatgtg tttggggtcc atcatctcta actgtctgac caaatttaaa
    24421 ttttctaagc attggtataa atattggtcc tattataatt actattaaaa acgcaatcaa
    24481 tgcggtgtac gtaagctctg ttattcctaa catcataaaa ctcctctccc ttgtcaatta
    24541 actcattagt ttaagcttat ttatttagat attcgactat tttttctaat ctcatgcctc
    24601 ttgagccttt tactaaaata gtgtctcctg tttttactaa ttcatttaac ttgttaaaca
    24661 catcttctct attctcaaag tgatatatat tggctgaatt gaatcctaat tgttttgctt
    24721 cttctccaat aaacactgaa ttttcaccta tagttattat aatatcagta ttattcatag
    24781 atgattttcc aactaatcta tgaccatact ctgaaatttc acccatttct agtatatctc
    24841 caagtatcgc tacccttcta cctttatatc ttccaagtat ctttaaagca gcatccatag
    24901 aatctggact agcattatat gaatcattaa ttattgttaa tttttcattt tttattatgt
    24961 ctagtctcat ttttgtccct ttaaaatttt ttaaaccttt ttttatataa tctaaagaaa
    25021 tatttagaca caaaccaact aatatagcag acattgcatt atatatatta tgttctccta
    25081 ttgttggtat aaatatttcc tcttttttcc catttattac acatataaat gttaaactat
    25141 cctcttccat agtataactt tcacaataaa catcattgtt tttattaaag ccaaatgttt
    25201 ttaacttata ttcatgttct ttttctttta atgtagataa aaacttatca tctccattta
    25261 caattaaagt atttgtttca tcaaagtttg aagtaatctc catctttgct ttcaaaatac
    25321 cctcttgtga acctagtttt tctacatgtg ataatcctat attcgatatg actgcaattt
    25381 taggatttac tatatccact aaatatttta tttcattgaa tccagacata cccatctcta
    25441 ttacagcaca ctcatgttct ttatttagat taaataaagt caatggaact ccaaaatgat
    25501 tatttaagtt tttttcattt tttagtatat ttaactttgc agatatggca gagtaaatca
    25561 tatctctagt agtagttttt cccacactac ctgtaacacc tataaatgga atatcaaatt
    25621 tttctttata gtattttgaa atatctccta gagccaaaga tgtatctttt acctctatta
    25681 aatttatatc cgaactttct aattttattc cattactttc attttttata aaagttttac
    25741 atccactatc atatgcagac ttcataaatg tatgcccatc taaattttct ccaactatgg
    25801 ctacaaatgc attttgggca tttgcctccc tgctatctat aactatatca cttacacagt
    25861 cattataatc tccatatatt aacttcccat tagttgcaag tactaactct tttattgtta
    25921 agcactccat tattaaactc ctcctttaaa accttagcta tattctctta gcaacaaaag
    25981 gcaatgtata ccgttatata tacattgcct acgataactc ttaactttat aattatatac
    26041 tatatttctg tttttttgaa gatgtttatg ggtatatgtt aattatctgt tactttttag
    26101 ttttcaaaat atagggttat acttgagtct tcattaactt ttactccagg ttttgggaat
    26161 atatccttta cctttgtatc ttctggaagt tctatatctg catctaagtc tggttttaac
    26221 ttcgcatcct ccaatgcctt tacagcatct cctattttta gatttcttac atctggcact
    26281 ttaacttgct tcttttcata ctcagctttc tcttcttctt tatatactgg tttaactccc
    26341 aaatatttta aagagtcatt cattatttct ttcactattg gtcctgctgt agtactacca
    26401 aatgcactta ctcctgttgg ttcgtcaaca atagcaagaa ctactatttg cgggtcatca
    26461 caaggtgcta ttccaacaaa tgaacatata tattttcctg gagcatactt accatctata
    26521 actttttgag ctgtacctgt tttaccacca agtctataac ctggtatata tgctatcttt
    26581 cctccacctt cagttacaac tgattctgca atttctaaca tttttttaga tgtttcttta
    26641 gatataacac ttcttacttt ttttggtttt actgtttcag ttatatttcc tttattgtca
    26701 gtataagatt ttaccactct tggttgcatt aaatctccac cattagctat tgatgatata
    26761 gctgttataa gttgtattgg agttactgat atagattgac caaatgatat tgtagccaat
    26821 tcaactggtc ctacattttt ttcgttatac agtattccct tagcttctcc tggtaaatct
    26881 atacctgtct tatccataag accaaatgct tctatataat catacatttt tccaacacct
    26941 aatctgcttc ctaactcaac aaatactggg ttgcaagagt tttgtactgc ttgtttaaat
    27001 tcttgtgttc catgaggtct gtaatgtctc caacatttaa tctttcttcc tccaacagtt
    27061 acactacctg tacatgtgaa tttttctcca tctttaatta ctccttcttc aagagcacta
    27121 gaagatgtaa taagcttaaa tgtagaacct ggttcatatg tatcacttac tgctggattt
    27181 ctccacattt gataatagcc ttttatttta tctttatcat tatatttttc aagttcttct
    27241 tgataatatg gatatattgg cgttcttgaa tcattagggt cataatctgg ttttgatgct
    27301 aaagcaagta tatcacctgt tttagggttc atagctattg cagtaacttt ttttgcatta
    27361 tttaactcat aagctttctg tactgctttt tcagtatagt gttgaattac ttcatctata
    27421 gacaacacaa gaccattacc ttgtacaggc tcataatatt tttccattcc ttgtggtatt
    27481 tctcttcctg aagcatcagt acttactata agttttccag gctttccttt taacttttta
    27541 tcatattgca tttctacacc agatatacct gttgcatctg aagaagtatg tcctaacaca
    27601 tatggtgcaa aattcccata tgggtaatat ctttgattat cttctgccac ccatattcca
    27661 cttaatttag catctcttat ttgacttgcc ttatcatcat ctatccatct ttttactttt
    27721 acaagagcca tatttttttt gcttattaaa gcgtaaatat ctttataatc ttcatccaaa
    27781 atctcagcta ccttttcggc tgcttctttt ttatcttcta cttccacagg tttacaccaa
    27841 acagtatact tagttacact tactgctaac tctttcatat ttctatcata tatagttcct
    27901 cttttgggtt ctattggtat atctcttgtt tgttgttcta gtgcttttgt actcaaccaa
    27961 tttcccttca taagttgtag atacccagtc ctaataacaa ggcaaaaaaa caatgcacat
    28021 gctagaataa gaacgagtac cagtcttttc ttacttatcc tctttacttt tctcaaatct
    28081 attcccccta gttatccaca tctatatact ttatttgtcc atcttttgga tagtccatat
    28141 tgagcaattc cttagccttt agctcaattt cttttattga tgttttttca gtttcttttg
    28201 cctttagttc ctctaatact atctcttttt ttcttaaatc tttttgtaaa taatagatat
    28261 cgtacttcat ttccgagatt tttgaataac cacatagatt caatattatt actgaaaaag
    28321 ttgcaatcag taaaatgcat ttagctccta tttttttctt atttctcttt ttataattat
    28381 tctttttttt atttttatat tcatttagat tatgtatttt ataatttttt ttcaaattct
    28441 caccccgata gtatatagtt taaattttcc tctatactat atttgttatt aatcatattt
    28501 cttcctctaa aatttcatcg ctaaattaat acactattac acaatctaat tgtatttttt
    28561 tctatacaca acaaaaacct ataaattcac ttatactttt atatgaatct ataggttaga
    28621 tattaatttc tatttatttt tatcacttct agtattttta gataatttct tccataaata
    28681 taatatgtaa ctagctatct actctctgat tcctaagttc tccattatat tacttaatac
    28741 catctatatc tatattttta tatctaaatt ttcattatat atctcaataa caaagaacat
    28801 ttcttatagt gttaattgtt ttttaattgc tttaccagtg caacaaaata tataatactt
    28861 tttaattaat tataataatc ttatatataa gattattctt tagccttata actacttata
    28921 ttttttctgc tactcttagc ttagcacttc ttgaacgtgg atttacttct atttcttcct
    28981 cactaggtaa tataggtttt ctagttatta ttttcacctc agattcttta tcacattggc
    29041 atataggtaa atgttgtgga catatacaat ctgatgctaa atgcttaaat gcatttttca
    29101 ctattctatc ttctaatgaa tggaatgtta ttatacatat tcttccacct tcattcatta
    29161 tagaagaagc atcatttatc atttttgtaa ttactccaag ttcattattt acttctattc
    29221 gtattgcctg aaaagttctt ttagctgggt gaggtccatc tattctagct ttcttaggga
    29281 tagctttttt aattacatct actaattctc ctgtagtttc tattggtttg tttgctcttt
    29341 cttcaactat aaatttagct atacgcttag cccagttatc ttctccataa tcttttatta
    29401 tcttagttaa ttcatcttct gtataagtat ttacaacatc atatgcagaa aactcacacc
    29461 taacatccat tctcatatca agtggtgcat cttgcatata agaaaatcct ctatcagctt
    29521 catcaagttg atgagatgaa actcctaaat cagctaatac accatcaatt ttgtaaactc
    29581 ctattttttc taattcctct tttatatttt caaaattgct gtgaacaaac ttcactctac
    29641 tttcatattc actcaatctc tcttttgctg tgcttatagc atttttatct tggtcaaacc
    29701 ctataaataa tcccttatct gacagttttt ttactatttc tttagaatgt cctgctcctc
    29761 ccatagtaca gtcaacatac acgccatctg gctttatatt taaattctct atacactcat
    29821 ttaaaagaac tgatacatga tgaaattcca tagttacctc ccttaaattc actttttaaa
    29881 ctattcttca ctaatattat ttttattttt ctttgataaa taaacatgtg caattatgcc
    29941 accaataact ccaattagac ttataacttg tgccattcta agtggtccta acattaaact
    30001 atctgttctt aatccctcta taaaaaatct tcctattgaa tataaagtta tataagtaac
    30061 tataacttga ccttcatatt ttttattttt tctaaatagt aataaaacta taaatattcc
    30121 aaaatcccat atagactcat ataaaaaagt tggatgtacc tttactccat caaccattat
    30181 tccccaaggt aaattggtag cacctccatg tgcttctcca ttaataaaat ttccccatct
    30241 tccaatagct tgtgctaaag gcattccaag tataactgta tcagccattt ttaagaagtt
    30301 aatattttta atcttagtgt atatataacc agctaatatt ccaccaatta accctccatg
    30361 tatagcaagt ccaccacctc taaaattaaa aatttgagac atattttgag catagtaact
    30421 ccaattaaat acaacataat agattcttgc acagattagt ccaactggaa ttgctattat
    30481 agctatattt aatacatcat cttctttaat tcccactctt tttgcttctt ttattgctat
    30541 taaagttcct aaaatcattc cacatgccat aagtattcca taccacatga tgtctatgcc
    30601 aaacagtgta aatgctactc tatccataac gtcacctctt tgtattaatt gaaaatattt
    30661 ccaaagcaat atgctataat tacaattaat tgtaattata ctaaattatg ataacacatt
    30721 aatgttaatt ctacaaaaaa tattaaaatt tattctacaa ctccctacac tatattatat
    30781 ctaattacta taaaattttt taaactgaat ctctcatgac taaagttaca agtgtacata
    30841 gatatttttt aatcaaaaag attgatgcat atatacatca acctcttcta ttccttatct
    30901 atagctctta aatttttacc tcaatttata taaatatatt ttataaattt gttttaccaa
    30961 aataaacatt taaaaacata aactaatact taattagtta ttcttattat gattaagttt
    31021 ctatttaggc tctacaatag aaataacaca atactcttct ttaaaatgct cttttaatct
    31081 ttcttcaact tcctcaaaag taatatcttt aataacatct aaataatcca atagatttat
    31141 atccttaaaa acataagata taaaactatt acctataaag tttatagaat caaagcactt
    31201 aataaatgac cctatttttt tctttttagt tctctcaaac tcttcttttg ataatccttc
    31261 tttttttgat ttttctatat aatcaagaat tatttctttt acttttttag gctctttaga
    31321 atctccagct attatgctaa aagcataatc cacttgagac gaaaatccag ctccaaaatt
    31381 ttcatttatt aaaccttgca tatacaaatc ttcataaagc ttacttcctt ttttaaataa
    31441 catccctaca agtatatcag ttacaatctc tttacgtaaa agttctttcc cttttaaacc
    31501 aacattgcta tctttaaaac ctatgttgaa cataggcata gatataggga atttttctat
    31561 aacctctttt tcttttacac tttctggttc ttctggataa aatctttcta tactcttaga
    31621 gagtctgtct actttgtaat tgtttgactt tttagttaca tctataactt tttctacatc
    31681 taagtcacca acaacaaata aagccatatt tcctggatta tagaaagtat tataacattt
    31741 atatagctct tccttagtta tcttatatat actatcaaca gtacctgcta tatcaattct
    31801 agcaggataa tttacataca ttgccttcaa gcaattaaag tatacattcc aatcagggtc
    31861 atcattgtac atttttatct cttgtgctat tatacctttt tccttttcaa cattttcatc
    31921 tgtaaaataa ggtgtttgaa catagtcaat tagatgttct aaactctcat aaaaattttc
    31981 agtagcagaa aataagtagg ctgtcattgt aaaattagta aatgcatttg catttactcc
    32041 taattttgaa aatttatcaa atgcatctcc tccatcaggt tgctcaaaca tcttatgctc
    32101 tagaaaatgt gcaatacctt cattcactct aattttctta tcttctccaa ttggtacaaa
    32161 ttctaagtca tttgaaccat aattagtagc caaaatagca tactttttca taaaacccct
    32221 tttaggcata aaatatacat ctaatccatt ttgcaatttc tcataataaa cttcttcttt
    32281 caatatatca ttaactattt tttccataga catctcctcc attaatttct caagaagtat
    32341 acagtatcaa gctctatatt ttttatagct tcaactatat ctttttttgt aaccttttct
    32401 atactagata ttatgtcttg tacttcagaa tttgtctttg ccatagactg agagaaaaca
    32461 aaatcagaca taccacctat attatcagtt atagatttca tagaattaac taaagcaagt
    32521 ttactgtttt ctaattcttc atcagatata tcacctgctt ttacactttc tacttgtttt
    32581 tttatcaatt ctacagcttt atcataatct ttagtttcta taccagatga tataaacatg
    32641 atacttttat acttctctat tgaagagaat atatagtaac ataaactttc tttttctctt
    32701 acattaacaa ataatttaga gtgtggacca ccacctaaga cattgctccc aactactaaa
    32761 gcataatatt tatctatgtc tgcataatcc acatttgctc tataacccat aactaactta
    32821 ccttgagtta tttccatttg ttcatctata acttttacct catccacatt ttttataaaa
    32881 tctgctcttg gtatatttat aatttcttct ctttcaaatt ttagattttt acttattata
    32941 tctacaactt tatcttcatc aaaattacct tctacaacta tatctatagg tgatgtttta
    33001 agtatgtttt tataatgttc atataactct cttgaagtta tactatctat ttcatcttca
    33061 taaccaaatt cacttacact atatctctct cctttacaca tagcctcaat acatttatct
    33121 tgcgcatatt cctttttatc atttattatt gattgtatct tttctcttaa attctctttt
    33181 tcaatattta aatattcttc tttaaaccca ccatctacaa caagcgtatt atttattact
    33241 tcattaaaaa attctactac ttcttcaaat atactctcat ctaaatactt ttcacttatg
    33301 ttaattactt taaaactcaa tacttgacgt tctccacgtt ttacagcatc agctcccata
    33361 gatgaaccat ataaatcatc taatttattg gatattgctc tagcactagg atatttagcg
    33421 cttccacttg ctattattga tggtattaga gcattttttg tagtttcttt ttcatccaat
    33481 agtctctgaa catataaact tataagattc gatttaaact tttctgtttt tatcaaagta
    33541 agattaatat tattacctaa atttattatt tttttagtat taaccatatg tacctcctat
    33601 ttttatttaa acatcaattt taaaattgaa atttatttat tttttaatta catatactca
    33661 catataaaaa ccttctattt aattatatcc tttttattat tttctaaaag caatattttt
    33721 atttaaatta aatacaaata ttatttaaat caatatgctt taaagtctag tttacaagct
    33781 tgatatatat gaggtataat caatatgtat gtctataatt aaaacaattt tgcaatccta
    33841 tagattgatt gtttaaagtt atataaagaa tataaaaaag aataagagag aggacgatta
    33901 atatgaaatt aggtatagtt ggtttaccaa atgtaggtaa aagcacacta tttaacgcaa
    33961 taactcaagc aggtgcagaa tctgcaaatt accctttttg tacaatagac ccaaatgtgg
    34021 gagttgtatc tgttccagat gaaagattaa acaaactaca agaattatat aattctgaaa
    34081 aaatagttcc tactgctata gaattttgtg atatagcagg tttagttaga ggagcttcta
    34141 aaggagaagg tttaggaaat aaattcttat cacatataag agaagttgat gcaatagttc
    34201 atgttgttag atgttttgag gatgaaaatg ttgtacatgt agatggttct gtagacccac
    34261 ttcgtgatat agaaactata aacttagaat taattttctc agatatagag attcttgaaa
    34321 gaagaattaa taaaactcaa aaagctgcta aggctgataa aactttaggt tctgaacttg
    34381 atttattaaa atcaataatg tctactttag aagaaagtaa gtgtgtaaga actatggaat
    34441 ttacagaaga tgaacaaaca tttgtaaatt cattggattt acttacatct aagcctgtaa
    34501 tatatgcttc aaatgtatca gaagatgatt tagctgataa tggagaaaat aataaatatg
    34561 ttcaacaagt taaagcattt gcagaaacag aagatgccga agttgttgta gtatgtgcac
    34621 aaatagaagc agaaatttct gaacttgatt ctgctgaaga aaagaaagag tttttagaaa
    34681 cattaggtct tgaacaatca gggcttgata agcttataaa atcttcttat gctttacttg
    34741 gtttaatttc attcttaact gctggtccaa aagaagttag agcatggact ataaaagttg
    34801 gaagtaaagc tcctcaggca ggaggaaaaa tacattctga tatagaaaga ggatttatca
    34861 gagcagaaac aatagccttt aatgatttgg ttgagcatgg tactatggct gctgctaaag
    34921 aaaaagggtt ggttaaatta gaaggtaaag aatacatagt taaagatgga gatgtaatat
    34981 tatttagatt taatgtttaa aatgattaat cgtatacact ttgactatta tatataataa
    35041 ctaaaaaact aagattataa taatacaata ttatcttata ataattatat agaatttaaa
    35101 aataaaagaa gagtatttca aatgatttgt ataaattatt atgaaaaact ctttttttga
    35161 tataataaaa ttacttacat atataacatt taaatttata aattatcttt ttatttttta
    35221 aaatatgtat aacacaccta tagaaattac tattattact at
//
LOCUS       DAGSHT010000019        35154 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGSHT010000019 DAGSHT010000000
VERSION     DAGSHT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.020"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..280
                     /locus_tag="MJB72_000888"
     CDS             <1..280
                     /locus_tag="MJB72_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8262013.1"
                     /translation="YSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(402..1136)
                     /gene="pflA"
                     /locus_tag="MJB72_000889"
     CDS             complement(402..1136)
                     /gene="pflA"
                     /locus_tag="MJB72_000889"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBY8262014.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(1296..3527)
                     /gene="pflB"
                     /locus_tag="MJB72_000890"
     CDS             complement(1296..3527)
                     /gene="pflB"
                     /locus_tag="MJB72_000890"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBY8262015.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            3820..4881
                     /locus_tag="MJB72_000891"
     CDS             3820..4881
                     /locus_tag="MJB72_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBY8262016.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            4972..6585
                     /locus_tag="MJB72_000892"
     CDS             4972..6585
                     /locus_tag="MJB72_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8262017.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            6860..8845
                     /locus_tag="MJB72_000893"
     CDS             6860..8845
                     /locus_tag="MJB72_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY8262018.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDVECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            8850..9284
                     /locus_tag="MJB72_000894"
     CDS             8850..9284
                     /locus_tag="MJB72_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBY8262019.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            9313..9873
                     /gene="srlA"
                     /locus_tag="MJB72_000895"
     CDS             9313..9873
                     /gene="srlA"
                     /locus_tag="MJB72_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBY8262020.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            9937..10305
                     /locus_tag="MJB72_000896"
     CDS             9937..10305
                     /locus_tag="MJB72_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBY8262021.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            10330..10896
                     /locus_tag="MJB72_000897"
     CDS             10330..10896
                     /locus_tag="MJB72_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBY8262022.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            10937..11302
                     /locus_tag="MJB72_000898"
     CDS             10937..11302
                     /locus_tag="MJB72_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBY8262023.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            11403..12176
                     /gene="srlD"
                     /locus_tag="MJB72_000899"
     CDS             11403..12176
                     /gene="srlD"
                     /locus_tag="MJB72_000899"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBY8262024.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            12525..14795
                     /locus_tag="MJB72_000900"
     CDS             12525..14795
                     /locus_tag="MJB72_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY8262025.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMTSNKVNYVSDELDMTSNKVNYVLDELDITSNKIDYISNELYNL
                     SNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMF
                     IPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVE
                     KRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKN
                     LGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
                     VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFG
                     GQVTYNESMVEIIRQN"
     gene            14883..15218
                     /gene="spoIIAA"
                     /locus_tag="MJB72_000901"
     CDS             14883..15218
                     /gene="spoIIAA"
                     /locus_tag="MJB72_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBY8262026.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            15232..15660
                     /locus_tag="MJB72_000902"
     CDS             15232..15660
                     /locus_tag="MJB72_000902"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBY8262027.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            15683..16447
                     /gene="sigF"
                     /locus_tag="MJB72_000903"
     CDS             15683..16447
                     /gene="sigF"
                     /locus_tag="MJB72_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBY8262028.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            16727..17164
                     /gene="spoVAC"
                     /locus_tag="MJB72_000904"
     CDS             16727..17164
                     /gene="spoVAC"
                     /locus_tag="MJB72_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBY8262029.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            17331..18344
                     /gene="spoVAD"
                     /locus_tag="MJB72_000905"
     CDS             17331..18344
                     /gene="spoVAD"
                     /locus_tag="MJB72_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBY8262030.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            18358..18720
                     /gene="spoVAE"
                     /locus_tag="MJB72_000906"
     CDS             18358..18720
                     /gene="spoVAE"
                     /locus_tag="MJB72_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBY8262031.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            18903..19919
                     /locus_tag="MJB72_000907"
     CDS             18903..19919
                     /locus_tag="MJB72_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY8262032.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            20294..21136
                     /locus_tag="MJB72_000908"
     CDS             20294..21136
                     /locus_tag="MJB72_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBY8262033.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            21151..21615
                     /locus_tag="MJB72_000909"
     CDS             21151..21615
                     /locus_tag="MJB72_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262034.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            21602..22774
                     /locus_tag="MJB72_000910"
     CDS             21602..22774
                     /locus_tag="MJB72_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8262035.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            22818..23753
                     /locus_tag="MJB72_000911"
     CDS             22818..23753
                     /locus_tag="MJB72_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBY8262036.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(24124..24849)
                     /locus_tag="MJB72_000912"
     CDS             complement(24124..24849)
                     /locus_tag="MJB72_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8262037.1"
                     /translation="MLESKIRTPEEIKRYMLDLKKWLAQERSKPVEEMSDFFSKRIDI
                     YDNVHLANWTEEYAHIADYFDTPLSSLLDIGCGTGLELASIYRKFPNVQITGIDLSKE
                     MMSKLKEKYSDKNIDLILADYFEYPFEQEKYDAALSFETLHHFKYQKKQKIYKKLFQT
                     IKPGGYYVECDYIACCLEEETICLEQYEFKCKENHVPDDVFVHIDIPLTIEHQIHLMK
                     NAGFRAVYVLHENNGTMIIKAEK"
     gene            complement(24867..25433)
                     /locus_tag="MJB72_000913"
     CDS             complement(24867..25433)
                     /locus_tag="MJB72_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8262038.1"
                     /translation="MPPKPIITKQDIINAAIQLVRENGISSVNARNLAKTLNCSTKPL
                     FRIYTNMDEIKEDIKIELDNYYNSFMESKINDENRLLSQGLAYIEFAQNEKMIFNTLF
                     MNMTMKGSSLQDIIHAKWNRITIENAKKVTGLSIEKSEMLFINFWLYSHGVATQIISN
                     GIDIPLDMVQQLLENAFERFSLDITKTE"
     gene            complement(26344..26742)
                     /locus_tag="MJB72_000914"
     CDS             complement(26344..26742)
                     /locus_tag="MJB72_000914"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBY8262039.1"
                     /translation="MKWKELYNKARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(27041..29734)
                     /locus_tag="MJB72_000915"
     CDS             complement(27041..29734)
                     /locus_tag="MJB72_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBY8262040.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     SATPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            29948..30646
                     /gene="yunB"
                     /locus_tag="MJB72_000916"
     CDS             29948..30646
                     /gene="yunB"
                     /locus_tag="MJB72_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBY8262041.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(30858..31865)
                     /locus_tag="MJB72_000917"
     CDS             complement(30858..31865)
                     /locus_tag="MJB72_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBY8262042.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            32093..32977
                     /locus_tag="MJB72_000918"
     CDS             32093..32977
                     /locus_tag="MJB72_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8262043.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            33179..34729
                     /locus_tag="MJB72_000919"
     CDS             33179..34729
                     /locus_tag="MJB72_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY8262044.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETEDLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            34758..>35154
                     /locus_tag="MJB72_000920"
     CDS             34758..>35154
                     /locus_tag="MJB72_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HBY8262045.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLII"
BASE COUNT        13089 a         4437 c         6006 g        11622 t
ORIGIN      
        1 atattcatct ttaaattacc ttaaagaact gtctgttaat ataataaaaa ttgaaagaag
       61 ttttgttaag aatattacat ataatagtta tgaatatact tttcttgaat atattattaa
      121 attagctcat attataaatt taaaagtttg tgttgaggga atagagtctt atgaagagta
      181 tgatatagta aaaagcttag gggtagatat tattcagggt tttttatttg gaagacctgt
      241 aagtgcctct gaattttata aatttcaact atcaaagtga tgtatataaa atgagatata
      301 tagtatataa aaatagggca aagtggaagt tttgaagttt attaaaaatt tacactttac
      361 cctattttgt tatgtcttct ataaaggatt aatatactac actaactatt atatactttc
      421 actccaaatt tagagattat ttcactagct ttttggacat cttccttagt ggcatcagga
      481 acatctttta gttcataatc gatacctaaa ctttcccatt tgtgtattcc catagaatgg
      541 tatggtagca attcaaatct ttctacattt ttcaatgtag ctacaaattg acctagtttt
      601 tctaaattat ctatatcatc agtaatgcca ggaactaaaa catgtcttat ccatacagga
      661 atatttcttg attctaagtg tctagcaagt tttaatgcct tatccatacc aacaccagtc
      721 aaatcaatag atttttcctc aaccatatgt tttaaatcaa gtaaaaccaa atcagtatta
      781 tctaaaactg ggtctatagt ttctatatct acaaatccag atgtatccaa gcaagtgtgt
      841 atatcatttt cttttgcttt tttaaataat tcacttaaaa attctggttg aagagtggat
      901 tcaccaccag aggcagttat acctccacca gaaaacttca taaaagaagt atacttaaga
      961 gcatccgtta ttatttcatc aactgtgtat tcttttcctg atttaatatc ccatgtatcc
     1021 ctgttatgac agtatttgca tctaagagga cacccctgaa aaaataatat atatcttatt
     1081 cctggtccat ctacagtacc aaacgtttct atagaatgta tctttccctt aatcatagag
     1141 ttcacctcac aatatatttt aataacctta tcaatagtta taatatttct ctaaaaaagg
     1201 ccatcttata ccagaaataa ttcaaatata agatagcctt ttatttttat tttttacaca
     1261 gtagtaatct tagttaaagt ataaaaattt aaagactaag ccattttacc gtggaatgtt
     1321 ctgtttataa catctaattg ttgctcttta gttaatttta tgaagttaac tgcatatcca
     1381 gatactctta tagttaattg tggatattct tctggatgtt ccatagcatc ttctaaagta
     1441 gctctatcaa atacatttac atttaagtga tgagcatttt gagcgaagta tccatccatc
     1501 attgcagaaa gattgtttat tctttcagtc atatcttttc ctaaagcagc aggtactata
     1561 gagaaagtat tagatatacc atcttgagca tgttcatatg gtaatttagc aactgaagat
     1621 aatgaagcta aagcaccatt agtatctcta ccatgcattg ggttagcacc aggagcaaat
     1681 ggagcaccag ctcttctacc atctggtgta ttaccagttt tcttaccata gacaacattt
     1741 gaagttattg taagtataga ttgagtagga taagaatttc tataagtttt attctttctt
     1801 attttattca taaagctttc aactaactct acagctatac tatcaactct atcatcatta
     1861 tttccgtatt ttggataatc tccttctatt tcaaaatcaa cagctattcc ttgttcattt
     1921 ctaatagttt taaccttagc atgttttata gcagataatg agtctgcaca aactgataat
     1981 ccagccatac cacaagccat agttctaaat atatctctat catgtaatgc catttctaat
     2041 gcttcataag aatatttatc atgcatataa tgtattacat ttaaagtatt tacatataat
     2101 gttgctaacc aatcagtgaa tggttcaaat ttactcataa cttcatcata atctaagtac
     2161 tcagaagtta taggttcaaa tcttggtcca acttgaacac cagatttctc atctacacca
     2221 ccatttatag cgtataataa agttttagct aagttaactc tagctccaaa gaactgcatt
     2281 tgtttaccaa ttctcattgc agatacacaa caagctatac catagtcatc tccccagtat
     2341 gctctcatta aatcatcatt ttcatactgt actgaacttg tatctataga tactttagaa
     2401 cagaaatctt tgaatccttg aggtaattta gttgaccata gaactgttaa gtttggttct
     2461 ggagaaggcc ctaatgtata tagagtattt aacattctaa atgagttttt agtaactaat
     2521 gttctaccat ctattcccat acctcctata cactcagtta cccaagttgg gtctccagag
     2581 aataagttat tgtaatcagg agttcttagg aacttaacca ttcttaattt cataacaaaa
     2641 tggtccatat actcttgtaa ttcttcttct gttacgattc cagcttttaa atctctttca
     2701 aagtatatat ctaagaaagt agatgttcta cctaaagaca ttgcagcacc attttggtct
     2761 ttaacagcac ctaaataacc aaagtataac cattgtacag cttcttttga gttagttgct
     2821 ggttttgata tatcaaatcc ataagattca gccatttttt taagttcatt taaagctgat
     2881 atttgctcag ttatttcttc tcttaatctg ataacttcct catccatgca agaaacttct
     2941 aatgatttct tttgctcatt tttatcttct attaaagcat caacaccata tagagcaact
     3001 cttctgtagt caccaattat tctacctcta ccataagcat ctggaagacc tgttattata
     3061 ccagattttc tagcagctct catttctgga gtataaacat caaaaactcc ttggttgtgt
     3121 gtttttctgt atttagtaaa tatatctttt atttctgggt ctaattcata tccaaaagct
     3181 tcgcatgaat tttcaaccat ctttatacca ccatatggca tgacagctct ttttaaaggg
     3241 gcatccgttt gtaacccaac tattgtttct aattctttat ctaaatatcc tggagcatat
     3301 gcagctattc cagaaacagt ttttgtatca acatctaaag taccaccatt ttctctttct
     3361 tttttaaata ggaccattgc ttcgtcccat aattttttag tattttcagt ggcaccagct
     3421 aagaaagagt catttccctc atatggagaa tagtttaatt gtataaactc tctaacattt
     3481 atttctttag tccatcttcc tgttttaaat ccttgccatg cattcattaa gtatttcctc
     3541 cttagattta aatgataaat aatactatta aaattaattt ttatatactt aatttatttt
     3601 atatattttt ttaaaaatat attttctaat ataataatag catattattc gtatcctgta
     3661 tacataaaaa ataaaaaatt gtcgctttaa agtataaaac agattatatg ttaataatgt
     3721 ggtgaaatta tacccatata taaataaaat aaaacaaata aatagctaat taaggagaaa
     3781 atatttttta ttttttgttt aggataaata aattttatta tggaaaaaag aaaaagcata
     3841 catcttcaca aactgaatat ttatttaata gggggaggtg atagattgtt tatcacagtg
     3901 gtattatttt tagtaggttt cctattaatt actaaaggcg cagatatctt tataaattgt
     3961 actgtagaga taggtaaaaa aacgaatata tcagaaatta tacttggagc tactatagtt
     4021 agttttgcaa caacattacc tgaatttaca gtgtctctac ttgcttctat tgatggtcat
     4081 actactatga gtttaggaaa tgcagttggt tctataatat gtaatacagg gttagcatta
     4141 gggttggttg tttttataag tccatttaat gtagataaaa aaatgttttt ttctaagtct
     4201 ttactattaa tagtatccgt tatagtttta attttattga gtttagatgg agttataaca
     4261 agaggtgatt cattattact tataataata ttaatctttt atatggttaa taattataga
     4321 agtgtcgttg gaaaatctga tactaaaaat agaaatatta aaaataacac tattaaagac
     4381 atatctacta aaaaagggaa aaattataga ggtttttccg tattagaaat tgtaaaaata
     4441 ctattacttt ttgctacagg attgataatg atgataatag gttcacaaat ccttatagag
     4501 agtggagtaa taatagctag ttttttaaat atacctcaag gtatagtaag tttaactata
     4561 atagcacttg gaacttctct gccagagata gtttcgtcga ttactgctat aagaaaaaat
     4621 catcatgaaa tatcagtagg aaatattttg ggtgcaaaca tattgaatat agtatcagta
     4681 atagctgtat cagcaatacc aaataacata ccaatactat ctcaaaatag acaattggat
     4741 ataccattca tgatattatt attattaata gtaataatac caacacttaa atctaataaa
     4801 cttagtagaa tacaaggtat attgatgtta ttcacatatt ttctgtacat atcaattttg
     4861 tatttcatgt atattattta actatactgt ttaaaatatt aattgcagaa tatcctaaaa
     4921 aagtatataa tggataggtt aagattaatt gataacggag aggaataata tatgaatata
     4981 acaaagtttg aagatttacc aataagtgag ggaataaaaa aagctatagc tgaaatgggg
     5041 tttgaagaac catctcctat acaagcacag tctatacctg cgattttgtc aggtaaagac
     5101 gttataggac aagctcaaac tggtacggga aaaacagctg cttttagtat ccctatacta
     5161 gaaacaatag acccaaataa tagaagttta caagctgtag tgctttgtcc aacaagagaa
     5221 ctggcaatac aagtatcaac agaaataaga aagttagcta agtattcgca cggaataaag
     5281 acattaccta tatatggtgg tcagccaata gatagacaaa taaaatctct aaagagtggt
     5341 gtgcaagttg taataggtac accaggacgt acaatagacc atatcaatcg taaaacatta
     5401 aagatggata atgtaaaaat gattatatta gatgaagcag atgaaatgct agatatggga
     5461 tttagagaag atatagaaat gattttaagt aagattccag aagaaagaca aacaactttc
     5521 ttttcagcta ctatgccaag aggaattttg gaattaacta agagatatca aaaagaccca
     5581 gagcatataa aagttgttag aaaagaactt acagtttcaa atacaaagca atactatata
     5641 gaaactaggt catcaaataa attagaagta ttatgtagat tagtagatgt atatgaccct
     5701 aaactatctg ttgttttctg taatactaag agaaaagcag acgaattggt aggagattta
     5761 caagcaagag gatattttgc agatgcatta catggtgact taaaacagac tcaaagagat
     5821 atagtaatgg ataagtttag aaatggtaca atagatatac ttgttgctac tgatgttgca
     5881 gctagaggta tagatgttga tgatgttgaa tgtgtattca actatgactt accacaagat
     5941 gaagagtatt acgttcatag aattggtaga actggtcgtg ctggtagaga aggtatgtca
     6001 tttacttttg tatttggaaa agaaatgaga aagatgaaag atatagaaag atatactaaa
     6061 tctaaactaa taaagcataa tataccaaca ataactgatg ttgaagagaa aaaagtaggt
     6121 actttctttg cacaagttaa acaaactata gaagaaggtc acttaactaa acaattacaa
     6181 tggttagaag gtttctgtaa tgatgaagat tatgcaatgg ttgatatagc tgctgcactt
     6241 gtaaaactat ctctaggtga agaaatgaaa gaagaaataa tagaagaaaa acctagaaga
     6301 gagcgtggag atagaaaagg tggtactgga gctaaagatg gtatgattag attatttatc
     6361 aatattggta gaaatcaaag agtacaagca aaagatatag ttggtgccat agctggagaa
     6421 gttggaatac caggtaaagt agtaggtact atagatatat atgataagta tacttttgtt
     6481 gaaataccta aaaaagatgc aaaaacagta attgaaaaga tgaaagatat taagataaaa
     6541 ggtaataaga ttaatataga aaaagcaaat aagaagaaaa aataacgtat agtatacaaa
     6601 actataatta gaaaatatat aaaataatat attggatatt taaaaagcct gatgtgtttt
     6661 caaaacagat agtagataat ctgtaagagt taacagcata aggcttttct tgttttatat
     6721 gatgttataa ttttccagat tattgtgaaa aatgtatatt agttcctttt tttcaaatct
     6781 gatttgaaaa agagaatatt aattatctat atagtttaga ttataataaa aaatgtaaga
     6841 taacaaaggg gagaaaaaga tgtcaatgac ttatatggac cctagggttt ttttgatttt
     6901 agaaatggtg ttagaagaag acacaaatac tctagaacaa ttttcaaatg ttttgggtgt
     6961 tagtactagg actatcagaa actatataaa ccagattaat cgagaattaa aaggtggtat
     7021 tgctaaaata gttaaaaatg aaaaaggtac atatagtata agaattgagg atgaaagtaa
     7081 attaattgag attgtaaatt ttaatagagg aaagctatca aattttataa atttaaattc
     7141 acctgatgaa agaataaatt atgttcttga tattttaact atgaccaaca atccaattac
     7201 catagatgat ttggctgaag agatatgtat aggaagaaca acattaataa aggatttaaa
     7261 aaaagcagac agaattttag ctgaatatga tttagaatta aaaaggaagc caaatatagg
     7321 tatgatttta gttggtgaag aaatggatat aagattatta atccttgata gattgtatga
     7381 aaattatatt gatgtcttgg aaaacgttaa tcagataaat ttagttaaag attatgatgt
     7441 tgaatgtctt agagatgaat taaaaaagtt atttaaaaaa gaagaactct acataacaga
     7501 acaggtcttg agagatgttg aaagatatat aatcatgtct gtcttaagaa atctaaatgg
     7561 gtacaaattt gaaaagatag ataagagatt tgaagtgata agatgttcag atgaatatac
     7621 tttaggatta aagctcaaag ctttattgga aaagatattt aatatagttt taaatgaaaa
     7681 agaaaccata tttttgacaa tgccattaat aggtagaaaa gctcctagta caaaattagc
     7741 acttagcagt ataaagataa atgatagcgt aaaggaagtt gtagatgaag taactagttt
     7801 tttattagag acctcaggga ttgattttaa agaagataaa aagcttatag aaaatttgga
     7861 atatcacttg tattttgcac ttaatagaat gagattcaac ataagggtaa aaaatcctct
     7921 tttacaagaa ataaaggaaa gatatacatt tccatatagt gttgcaaaga tagctgcaat
     7981 gataattgag gaaaagtttg acttgaaaat atgtgaccac gaaataggat atatagcatt
     8041 acattttgga agttattttg aaaaaaattc aagaaaagtt ctaaatgtaa atagggtagc
     8101 tgttgtttgt ggaacaggtc ttgggactgc acaacttatt agaataaaga taggaaaaat
     8161 acttggagaa agtgtagaag taaatacttt ttctgatata gaagctaaag ctaatttaga
     8221 tttattaaag tattatgatt tagtatttac aacagtagat ttcaaaacag acttagatgt
     8281 tccaattttt agagtaaatg cgttatttaa tgaagaaagt ataaagaaag agatagaaaa
     8341 tgcaatactt ttgaaaaata gtataaatct taagtctata aaaaagattg aaaaaaatat
     8401 cccttttata ggtaaattaa ttgaagagga taagttcttt atattgaata agagtacgtt
     8461 tatggaaaat ttagaagata tgttagaaaa attagttcat aaaggaatta ttgatataga
     8521 gttcaaaaaa aaggtattaa aaagagaaaa gaaatcttca acagcattag ataattatat
     8581 agcattgcct cactcagtta atactaatgg ggaaaatctt tatctggcat ttggaatatt
     8641 agaaaaacca gtgatttggg atacaaagga aattagaata ataatattga tgataattcc
     8701 agataagaat gtagatagta cagacctgat aataaaaagt tatgaagaag tacttgaagt
     8761 agggagagat aaaaagatgg tagaagaact tgctaaggca aaatcatttg atgaatttta
     8821 taaaataatt accaagagga ggtaataata tggacccagt aatttttatt atagcatcac
     8881 ttattacagt gtatttatta aatatttttc ttggatactt acagcttaaa gattttaata
     8941 aaaattatat tgaactaaaa agaaaaggaa gagtggcaat tggcagaaaa aaaggaagaa
     9001 tttcatcggg tacaattgtg ttgatactta taaatgatga tggtgtgatt gtagagacta
     9061 gaaaaatgca aggtgttaca gtattggcaa gagtaaagtc atttgaaggt ttggtaggaa
     9121 agagcttaga gtatatagaa aaatcagatt taactgttta caataaacta cttaaaaaag
     9181 caatactaga tgcagtcaaa cagtacataa catttgagaa aaataaatgt gaagatacaa
     9241 ataatctgag tactgatgaa gagtgtagga tggaagtagt ttaaaaaaat atatttataa
     9301 ggaggagtta atatgacgca attttttcaa gtattagcaa gtggtgcaga tagttttatg
     9361 aatttattta gggcaggtgg gcaacaattt gtaggttttg taacagatat agtaccactt
     9421 ttagtagcat tattagttac aatgaatgca attatcaact ttattggtac agatagagtc
     9481 gagagacttg caaaaaaatg ttctgcaaat atatttacta gatatttgat attaccagtc
     9541 cttggtacat ttgtatttgc aaacccaatg acactatcat taggaagatt cttgccagaa
     9601 aagtttaaac ctagttactt tgcagcagca agcttttcat gccatacaat gaatggatta
     9661 ttcccacata tcaatccagg tgaactattt atatatctag gaattgcaaa tggtataaca
     9721 actttgggat tctcaacagc agatttagca gttagatatt tgttagtggg tatagttgca
     9781 aactttatta aaggtgtagt tactgactat acaacaaaat ttgtagagaa acaacaagga
     9841 atagcattaa attcagatat aaagattgga taattacttt aaggtataaa taaatttttc
     9901 aaataaaaag atagtgtata gaggaggata aattatatgg catttaacaa agttaaaata
     9961 gtaaaaggtt ctggaggatg gggtggacca ttgataatag aaccaacaga aaagaagaat
    10021 aaggtagtat atgtgactgg gggagcaaag ccagaaactg cagtaaaaat agctgaactt
    10081 acaggatgtg agttaataga tggattttca cagggtgtta aagatgatga aatcgcatgt
    10141 gtaataataa attgtggtgg aacattgaga tgtggtatat atcctcaaaa gaaaataccg
    10201 actataaata taatgaaaac aggaaaaagt ggaccattag caatgtttat aaaagaagat
    10261 atatatgttt caggggtaaa accaacaaat gtagagcagt tataattgag gtagtaggag
    10321 ggaagaaata tggcttatga tactaaaaag aagataagtg agcagagtca aggaattgtt
    10381 gcaaaaatag gtatagcact aggaaatgtg acaagtactc tttttcaagc tggtagggat
    10441 actatagata cattaataca tactatactg ccttttatgg ctttcgttgc agtcctaata
    10501 ggtataataa atcaatcggg tataggaaat tgggtagcca atttacttac tccattaatc
    10561 ggaaatgttg gaggtttagt tgtcttatca ataatatgtt catttccgtt gttgtctccg
    10621 tttcttggac caggagctgt tatagctcag atagtgggag tattggttgg tacagaaata
    10681 ggaaaaggaa atattccacc aagtttagca ttaccagcat tgtttgcaat aaactcacaa
    10741 gcagcttgtg actttatacc agtaggtctt ggtcttgcag aagctgaacc tgatacagtt
    10801 caagtaggag ttccgtcagt tttatattct agatttttaa caggtgcacc aactgttcta
    10861 ttagcatggt tagcaagctt tgggttatat aaatagaaga atactttata aataattaga
    10921 gagaggttca gttgatatgg aattaatata cagtactcaa ataaataaaa taggagataa
    10981 tgcaaatgaa tttttacaac ataatatgtt tgtaactttc aaagataatg ctcctcaaga
    11041 gttagaaaat tactgttata tacattctga aaataattta gtaaaagaaa tacttacgga
    11101 tgatgtgcta tcaataaata atgttgatta caaaattact tctgttggtg agttagtaaa
    11161 ccaaaatctt tcagaattag gtcatataac atttaagttt acaggtgaaa aagatggagc
    11221 aattggagga accttatatc ttgaagaaaa agaaatagca cctattggcg taggtactat
    11281 actaaaaata attagaaaat aaaatttaaa acgaatctaa tcatattata agtaaattat
    11341 agataaaagt caatttaact aatcaagtta acttttaatg tgataaaaaa ggggaaatga
    11401 aaatgagaaa agtggcttta gtcgttggag gaggaagaag tttaggtgaa tatttatcta
    11461 aacatttagc taaagtaggt tatgatgttg ctattgcaga tttaaatgaa gacaacgcaa
    11521 aaaaagtagc tgaagaaata aaaactgaat ttggttgtaa atctgtagga tatagttgta
    11581 atgccataga tgaaagacag gttataagta ctgtcaaagc tattgaaaat gattttgata
    11641 gaatagattt attagtgtac aatgcaggag tggctgtaag taaaaaaata actgattttg
    11701 gatacaaaga ttttaaattt attgtagacg taaatttaaa tgggtacttt ctatttgcaa
    11761 gagaagtatc taaagtcatg atagcaaaag gtattgaggg tagaataata cagataaatt
    11821 caaaatcagg tagagtggga agcaagcata attcaggata ttcagcaggt aaatttgctg
    11881 gagtaggact tactcaaagt ttagcgctag atttggcaga acataagata actgtaaatg
    11941 cactgatgct cggtaattta cttgattctg atatgtttga aagtttaatt cctcaatatg
    12001 cagtaaaatt gggaatacct gaatcagaag tcaagcaagt ttatatagat aaagtacctt
    12061 taaaaagagg gtgtagattt atagatgttg caaatatgct tacattttat gcttcaaagg
    12121 aagctgaata ctgcactggt caatctataa atattactgg tggacaagtt atgtaaggtt
    12181 tttatggagt gtctcaagat gaactcttta gttcatgagg cacttttttg tataaaatta
    12241 aatatatttt cagcatttaa acaatgaaaa agaggtttat ctttattttg agatagcctc
    12301 atttttgtaa ttaaaaaaca tagtgtttaa gtaaacatat aagattgtta gttttttaaa
    12361 agagaatttt ttaataatat tatatttatg aagtattttt aaacttaatt tgataaaata
    12421 tagctatgtt ttatatactg tagttttgaa catagaaagt ttaataaatt atagtatata
    12481 tgttaatttt taataaggta agaaagtaga aagaaggata ttttatggat ataataaaaa
    12541 aattaagtca tgggtgtaca ttagattgtc atgactgttg taaattcaat gtttatacta
    12601 aaggaaataa tgtagtaaaa attgaaggag ataaaaatca tccttataca aaaggtttta
    12661 tatgcaaaaa agggatggcc catttagata gattaaatca taaagataga ataaagactc
    12721 ctatgcttaa agttgatgga gtatggaaag agatttcttt tgataaagct atagaaataa
    12781 tggcagaaaa gcttacatac tataaagaaa agtatacatc aaagtctgtc atgcattatg
    12841 accaatatgg aagtgggtct gtgctaaaat acataggaga tatatttttt aatttttatg
    12901 gaggtgttag cagacataag ggtggtccct gttggagtgc agggatgcat gctcaaaaat
    12961 atgattttgg agtagctaag agtcatgcta tagaagatat gttaaatagt aaaagtatat
    13021 ttgtgtgggg aaaaaatcca gcatatacta caattcatac tatgcaaata attaaaaaag
    13081 ctaaagaaaa agggataaag atagtagtga ttgaccccat atatacaaaa acagctcaaa
    13141 tagcagataa gtatgtacaa gtaaatcctg gaacagatgg agctttagct atagctatgg
    13201 caaaaattat agtagaagat aaattatatg atgaagaata tataaactcg tatgttattg
    13261 gttttgaaga atataaaaaa tatttatctt cattggaatt aagtttttta attgatgaat
    13321 gtggtgttaa agagaatgat ataagagagt tagtggattt atatactaac aaatattcaa
    13381 gtataaatgt tggatatggg cttcaaaaat ataaaaatgg tggaaacaca ataagagcaa
    13441 tagatgcttt aggtgctata actggtcaaa taggatttag tggtggaggg gtaaactatg
    13501 ctaataaagt ttatccgagt gttataaatt ctgaccctta caatagccaa tcatatggtg
    13561 aagataggga gttttatgta agtaatatca gtaaatttat agaagaatct ttaaaaaata
    13621 catcaaataa agtaaattat gcatcagatg aacttgatat ggcattacat aaagtaaatt
    13681 acatatcaga tgaacttgat acaacatcaa ataaagtaaa ttatgtatca gatgaacttg
    13741 atatgacatc aaataaagta aattatgtat cagatgaact tgatatgaca tcaaataaag
    13801 taaattatgt attagatgaa cttgatataa catcaaataa aatagattat atatcaaatg
    13861 aattgtataa tttatctaat aagtcaatca aagataatat tcctataaag atggctgtga
    13921 taactaaaag taatatgctg aatcaactgc cagatttagt agaattggag agggtgtttt
    13981 caaaaattga atttaaagta tgttttgata tgtttataac ggatacagct actttatgtg
    14041 acatgtttat accgtgtaca aatacactag agagtgaaga tataatttat agttcaatga
    14101 ccaatccata tataacctat aatgaaagag ctgtaaagcc agcacataaa ttgatggatg
    14161 aatattactt ctttatggag ctagctaaaa agatgggatt aaatgattat ccttttgtag
    14221 aaaagagaac ttatttagaa aaagtgattg aaccacttaa gcgttttgac aaaaatctgg
    14281 atatagaaaa acttaaaaac aattacttta ctatacataa ccctgttgct tgggaagata
    14341 agaagtttga gactccatct ggaaaatacg agctttattc agaaagtata aaaaacttgg
    14401 gaataagtcc aacacctgta tatataagca ataaatataa agaaatagaa gataaaaata
    14461 tttcttttag attattaact aatcatcatg cagatacttt atttagtcag cattttatgg
    14521 acaaaaaatc tatagctcaa gcatatataa atcggagaat ggctaaaaaa gtaggtattg
    14581 aggacaaaga tattgtgata ttgaggtcaa aaaaagcaaa aataaatgta caaataaata
    14641 tagatgatgg agtaggtaat tatatagtaa aaatgtatgt tggttggtgg aagaaacatg
    14701 gaaatcctaa tagtttaaca gacactggta tatcagattt tgggggtcag gtaacatata
    14761 atgaaagtat ggtagaaata atacgacaaa actagttaaa tttcattgaa ggaataaaaa
    14821 tataattata gaattgatta aaagttacta tttttacaaa actattgaag gagggatttt
    14881 tgatggtaaa ttattcctta gaacataaga atctatacat agaatttatg tgctcagaac
    14941 tagaccacca tgtagccaat gaaataaggg aagaaataga taacttatta agtgtaaatc
    15001 aagtaaaaaa tgttgtattt aattttgaaa atattaattt tatggattct tcaggtatag
    15061 gcgtcatcat aggtagatat aaaaagattt ctaatgaagg gggacgagtt tcagtaatta
    15121 atataagttc acgagttaag aagatttttg atttatctgg gttaaataaa attattggca
    15181 tttatgatac gtatgaagaa gctctaagtt ctttgtaggg aggagtatgt gatgaataat
    15241 attatggaag ttaaattttc ggcaatatct gaaaatgaaa gtttagcaag agttatagta
    15301 gcatcctttg cagctaagtt agacccaaca gtagatgagt tagtagatat aaaaactgct
    15361 gtctctgagg ctgttacgaa tgctataata catggatatg aagaagacag ttctaagttt
    15421 gttttcttaa gatgtgagat agaaggaaat actataaaga ttatagttga agatgaaggg
    15481 tatggaatag aaaatgtgga gaaggcaatg gaaccacttt atacatcaaa accagaactt
    15541 gatagatcag gaatgggatt cacagtaatg aaaagcttta tggacgatgt agaggtaagt
    15601 tctgtaaaag ataatgggac tagaattgaa atgactaaaa aaataaatgt gcctaaataa
    15661 agtccaaaag tgaggtttta ttatggaagt aactgttgcc agagaagaaa aaaaacctct
    15721 tcttagtcat gaagagactc tagaacttat tgaaaaagtt caaaatggag atgaagaggc
    15781 aaaagaaata ttaatatcaa gcaatttagg tctagttagg agcgttgtat ctagattttt
    15841 aaatatagga tatgatagag aagatttatt tcagttaggt tctataggct taataaaatc
    15901 aatatataaa tttgacccaa aatttaatgt aaaattctct acttatgcag taccaatgat
    15961 tttaggtgaa ataaagagat acttaagaga tgatggtatg ataaaagtat caaggtcgtt
    16021 aaagcaaata gctgtaaagg caaagatgga aagtgaagct ttaactaaaa aattaggtag
    16081 agagccgagt atagaagaat tagctaaagc aatagatgta gaaaaagaag atttagtaat
    16141 ggctatggaa gctaatttca atgttgagta tttgcaagga gttattcatg aagaggaagg
    16201 ttctccaata tgccttattg ataaaataag catgaaagga gaaagtgaag aagaaaaagt
    16261 agtggataac atattgttaa aagacatact tggaagactg gataagagag agcgtcaaat
    16321 aatagttctc agatactttg aagacatgac tcaaagtgaa ataggagaga tgctcaatat
    16381 atcacaagtt caagtatcta ggatagagaa gaaagtattg tctaagttaa aagaatatat
    16441 atcgtgataa tacttatgat atgtagaata acaaaatata ataaatatat tactaaaata
    16501 aaatgtaata actttttatg ttaagttttt aaattattaa aagatattaa aataataaaa
    16561 agcattaaaa aagtaaaaat ataaaaacaa ggtgtcttta tggaaaaata gattttttct
    16621 ttattaggca tcttttttat tttttgacca aactttagat agtcaaaata aataataggt
    16681 ttattttttt aaaattaaca aataatactt tagagaggtg tttagtatgg ataaaaatta
    16741 taaaaaatat gtagaccaaa taagcccaaa acctacatat ttaaaaaact atacacttgc
    16801 atttattgta ggtggaatta tttgtatgat agggcaagca ataaatgatt tgtatatgaa
    16861 agttggaggt cttgataaac taggtgctag ttctgcaaca tcaataacat taatatttat
    16921 aggggcattt ttaactggat taggagttta cgacctaata ggaaaaagag ctggagctgg
    16981 ttctataatt ccaataacag gttttgctaa ctcaatagta tctccagcaa tggagtataa
    17041 aagagaaggc tatgtattag gtgtaggtgc aaacttattt aaaattgcag gaccagtttt
    17101 agtttatggt ataggttctt ctattttatg tggaattatt tattatattt ttaagatgtt
    17161 ttagatgatt tttatttaat ttaaatttta ttaattatga agaaggtata aaatctgaaa
    17221 atagctacta aagtaactaa ttgaaaaata attgtgcaga tttaattaat atttttaatt
    17281 aatattaaaa atttgaatag taaaatgcaa aattaaaaag gtgtgaaaat atgaaaaata
    17341 aaagaattgg aaaaagaaca gtcaagttag aaaataaacc aaccataata tcaactggaa
    17401 ctatagtagg tcctaaggaa ggtgaaggac cactaaagga ctattttgat atgattatga
    17461 ctgacgattt atatggtgaa aagacttggg aattagctga aagtaaaatg gtagagactg
    17521 catctcaaca agctatccaa aaggcaggta aaaaattgtc agatgtaaat tatatgctag
    17581 gtggcgattt aataaatcaa atagttccag catcatttgc agcaagagaa ctggctattc
    17641 catttttagg tatatatgga gcttgttcta caatggcaga aggtctttgt ataggttcta
    17701 tgcttataga tggtggtttt gctgatttag tactgtcagg tacatcaagt cactattgta
    17761 cagcagaaag acaatttaga ttcccacttg aacttggaaa tcaaaaacca atgacagctc
    17821 agtggacagt tacaggagca ggaagtgtat tattagctcc taatggagac ggtccaaaag
    17881 taaagtatgt tactgttggt aaagtaatag atgagggtat tgatgatggt aataatatgg
    17941 gaccagctat ggctccagca gcaatagata ctatctactc ttactttgaa gatactaatg
    18001 atgacccaaa tagctttgat ataattgcta ctggagattt aggtactctt ggaaaacaga
    18061 tagcagaaga ctttttaaaa gaaaagggtg tagatatatc aaaagtttac actgactgtg
    18121 gtatagagat gtttaacctt aaagaacaag atgtccactg tggtggaagt ggatgtggat
    18181 gttcagctac agtgtttgca ggatatatat atgataagtt gagaaaaaaa gaatttaaca
    18241 aagtaatgct tgtatcaaca ggagcactac tttcaccgac aagtacacta caaaagcaga
    18301 caataccatg tgttgcacat gcagtagtaa tagtaaatga gtaggaagtg atataatatg
    18361 ttaatggact atgtaagagt atttattgta ggtggaatta tatgtttaat agcccaagta
    18421 atgatggatt atttcaaaat gcaaactcca tatgttatgg taacgtatgt aacaactggt
    18481 gtagttttaa cttttctagg actttatgag ccattagtta agtttgcagc agcaggtgca
    18541 acagttccta taataggatt tggatattca cttgctacag gagtaataaa atcaattcaa
    18601 agtgatggtt ttttaggtat ttttttagga ggtacaactg ctacagctgg tggaatagca
    18661 gcagctatat tctttggata tattatgtca gtagtattta ctccaaaagc aaaaccataa
    18721 atctttatta aaagaataat aagtttttga aataataact ttttataaag caggtggata
    18781 tcttttagat tccacctatt ttttatataa agaagcgttt acataattga agttgacaat
    18841 tatttacaac atagtgtagt attatattat caaaataaaa tgaagtgaag taggggataa
    18901 agatggtaac tattaaagat attgcaagga atctaggtat cagttattca actgtttcta
    18961 ggtgcttaaa caataatcct aatgtatctg aaaaaacaaa aaagaaagtg gtagaagagg
    19021 caaatagact agggttccat tttaatgtaa atgcaagaaa tttagcaaaa aaagaaacta
    19081 acaggatagg ggtaatcttt tcaaataatt ttaatcatca agatactaga aagtttttta
    19141 gtgatataat ggatagttct ataaattcta ttgagactaa taaatacgat tttataatac
    19201 agcctaataa taacattagt ggtgatagta atgtatataa aatggtaaat ggacaaatgg
    19261 ttgacggatt agtaattgtg tctcaatcaa taaaaaaaga agagtatgat tttttaaaag
    19321 acaataattt tccacatgta tttatatatt ttaaaccatc ttttatagga gaagtagata
    19381 atttcttttg ggacgataac gtgtatggag gttatatagc taccaagcat ttaattgacc
    19441 atggtcataa agatataata acagtaactt cagatgataa gagttcaaaa atgcatgaag
    19501 atagaaccaa gggatatttg aatgctatgg aagaagcaaa tttaaagaca gaagtcatta
    19561 aatctaaaat ggattttgaa tctcaagttg aatttttaga aaaatacatt gataaaataa
    19621 aaaaagcttc agctatattt gtacaacaag atgtaccagc gatttctata atacaagagt
    19681 taaaaactac ttatggtata aatgtaccag aagatatttc cataataggg tataataata
    19741 ttgaattaat atcatatttt aggtcgcatt tatcaacaat agatgaccca agagagcaag
    19801 ttataaaaaa tggagtagat agtttagtaa atataataaa taaaaagagt acagaacaca
    19861 ttccaagaaa attatatcca aggttaataa tacgaaatag cgttaaaaga ataaagtagt
    19921 aagcttttaa gtatcaaaag tttattaaaa atattataga ttatgtgaag ttttttaaca
    19981 aataatataa ataaaaatta aaaggagtag gtgtatataa gtacattgta ctcctttatt
    20041 tattgagata gtttttataa tatgaatgaa taattttatt caattatttg aaaatccgtt
    20101 attttaacaa tataaattaa taaattattt gtattttata tcatgaatct ataatatttt
    20161 tttgattcat aactaaaagt atataaaaat gatgaataaa tatatattat acattatcaa
    20221 tgataggtta tataataaaa tcactaacaa ttatattata agaaaattat aaaaattaca
    20281 aggaggtata gaaatgaaag aaaaagcagg gattagaaat gccataatag cagcctttat
    20341 aatcacaata ttggcagaat taattggacc tatttcattt aaagttatgg gaattaatgt
    20401 aactttactt agtatactat gggctatatt cataggaatg gctgtttctc ctcatttatt
    20461 agggagagtt ataccagcct taaaaaagtt tataggggat aatgaaataa atgtatctcc
    20521 atatttatta tctttgacac tatatccatt agggataatg tttggaatta atgcagggcc
    20581 taaagttggt atattacttc aagcaggacc agcactttta ttacaagaat ttggtcatat
    20641 gggtacaatg ttgatagcat tacctgttgg agtagcattg ggtcttggaa gaagtgcatt
    20701 aggtggtact ttttcattat gtcgtgacac agcactagga attattggag ataagtatgg
    20761 attaaactca ccagaaggta taggaacact aggaacttac ataagtggta gtatatttgg
    20821 aacattatta ttttcatttt tagcaccagt gggtgtactt ttaggatttc atccatatgc
    20881 tttagcaatg gcatcaggaa tgggtagtgg ttcaatgatg ggagctgcaa cagcatcatt
    20941 aatgaatacg gttccacaaa tgtcagagca gattttagca tattcagcta ctagtggatt
    21001 gattacagct gttactggaa tatatataga attattctta tctttaccag tagccaacta
    21061 ctattatagc aaagtagctc cagttattga aagatttaga ggaagaaaaa caagaagaac
    21121 aagtaataaa ttgtaaagga ggatatatat atggaaaaaa cttttaagac tattatggag
    21181 cagataaaaa tagtattgtt tatatatatt attatttgct ttggacaaat gatagcactc
    21241 aagactcagg ctcaagattt gttgattgga agtgtatctg gatttttgtt agtgataatt
    21301 gctattataa taaagaattt tgttaaaaaa cctaatttac caggttttgc atgggctaca
    21361 ttagttgcat tcgttttatc gcttccaata tctccagtgg gggatatcat agcaacaaat
    21421 gtagggaaaa tcaattttat gtctacagta acaccattat tagcttttgc aggaatctct
    21481 gtaggtaacc aattagatat attaaagact ttgagttgga agttagtagt tatatcattt
    21541 atagttatga catctactta ctttggttct gcttttatat ctcaaacagt tttaagatta
    21601 aatggaatgc tttaaggagg tagtaaatat gaatgaatta aaagcaaaaa ttaaagagga
    21661 aataaagtta ttatctgaag agaaaaagac atcatttgaa aaagtttctg actatttatt
    21721 ttcaaatcca gaattagcat ttgaggagta taagtcacaa aaagcattat gtgatttgtt
    21781 agaagaaaat gaatttaatg taactaaagg tgtaggagga ttagaaacat cttttgaagc
    21841 tgtgtattca aatggaacta atgggaaaac agttgctttt ttagctgaat atgatgcact
    21901 tccaggaatg ggacatgcat gtgggcacaa tatcataggg acaagttctg ttggtgcagg
    21961 tatcatatta aaagaaatca tgaaaaaaca taatatagaa gggactgtaa aggtttttgg
    22021 aacacctgca gaagaaagag taggtggaaa aataactatg attaaagaag gtgtatttaa
    22081 taatgtagat gctgcattaa ttttacatcc aagtgatgct tcaatgcctg atgatatatc
    22141 atttgctcaa gttaatttaa aatttgattt tactggtaag gcatcacatg ctgctgcttt
    22201 tccttgggaa gggaaaagtg ccttaagtgg agtaatagca ctatttaaca gtgtaaatag
    22261 catgagatta catcttaaag attatgcaag agtacatgga attataactg atggaggtag
    22321 catacataat ataattcctg aaaaatctac agctatattt aatgtaagag ccttaagtat
    22381 agaatattta aatgagattt gtgaaatgct taaaaattgt gctaaaggag ctgcaatttc
    22441 tactggaact aatgtagaaa tagttcaatt agatgaaata tacaaagaga ttaagaatga
    22501 ttctgaatta gtgaatatag ttagacaaaa ttttgaagtt ttgggtgaag actatgttga
    22561 aagagattta agtcaaggta taggttcaac tgatacagga aatttaactc atgagatacc
    22621 tgcaatacaa gcatatataa aattaaaaga aaatactgca actcatacag atgaatttgc
    22681 tgttgcagct ggtggtgaag aaggcagagt agcacttata aaagcaatta aggtgttagc
    22741 aatgtgtgga gtagatattt tgtatagtaa ataatatata attttaaaat atattaagaa
    22801 aggaaatcgg attagacatg atattaaaac aagtaataga ggtttatgac attttagata
    22861 aagcaaatgc taatggggaa gaagtaaaaa cttacttaca aggttatggt gaagtagatg
    22921 taactgtaaa agaattgtct tcaagtaaag gttcaacaga tttagtaaag cttacaattc
    22981 ctgggaaaaa tggaaaatta aaaggtggag atgctcctac tcttggtatt ttaggtagac
    23041 taggagggat tggagcaaga ccagaagtta taggttttgt ttcagatggt gatggagcat
    23101 tggtagcaat tgcagtggct gctaaacttt tggatatgca acgaaaaggg gatatattag
    23161 atggtgatgt tgtaatttca actcatattt gcccagatgc tccaactaaa gaacactatc
    23221 ctactccttt tatggattct ccaattgata tgatgactat gaatgaaaat gaagttgata
    23281 gtagttgtga tgcaatactt tctatagata ctactaaagg aaatagagta atcaatacta
    23341 gaggatttgc aatatcgcca actgttaaag aaggctacat attaaaaact agtgataacc
    23401 ttttagatat tatgcagact gtaacaggga aatctccatt tgtattccct ttgtctattc
    23461 aagatataac accatatgga aataatctgt atcacttaaa tagtatttta caaccagcag
    23521 tagctacaga tgcaccaata gtaggagtag caattacaac agagatgcct gttgctggat
    23581 gtgctacagg tgcaactaat tttagtgatt tagattcagc tgggagattt gcaattgaag
    23641 ttgccaagct atttggtaga aataagtgtg atttctatga taaagaagag tgggaaatgc
    23701 ttattaagag atatggcaaa ttaaatagat ttcaaacatt tggtatacag taattagaaa
    23761 aatattaagt ttatataatt tgtgatataa atatctatac tatgtaaaca actagaaaca
    23821 aaagatatag tttaagactt aaaaaataaa atatttattt gattacataa ataaggagtg
    23881 ttctatttag gtaagcgcca ataagtagta taacattaaa gctaacctaa ttgttgatat
    23941 acaagattga aatgaaaaca gtgcgtatta taagaaaagg ttacgcactg tttttttcgt
    24001 cctgttatat agcagaacac tagtttttaa gggtatgagt ataaaacttt tttaaatttc
    24061 atatctacga attttgtaga ttgtccataa gcgatatgca ataattagga aaatattttg
    24121 agtttatttt tcagctttaa taatcattgt gccgttgttt tcatgaagta catacacagc
    24181 tctaaagcct gcatttttca ttaagtgtat ttgatgttca atagtcaaag gaatatcaat
    24241 atggacaaaa acatcatcag gcacatgatt ttctttacat ttaaattcat attgttcaag
    24301 acaaattgtt tcttcctcta aacaacaggc aatataatcg cactcaacat aatatcctcc
    24361 aggtttaatt gtttgaaaaa gctttttata tatcttctgc tttttctgat atttgaaatg
    24421 atgaagcgtt tcaaatgaga gggcagcatc atatttctct tgctcaaaag gatattcaaa
    24481 ataatctgcc aagattaaat caatattttt atctgaatat ttttctttaa gcttactcat
    24541 catttctttt gataaatcaa tgcctgtaat ttgtacatta ggaaattttc gataaatcga
    24601 tgcaagttct agaccagtac cacaaccaat atcaagtaaa gaagatagtg gagtatcaaa
    24661 ataatctgct atatgtgcat attcttctgt ccaattagca agatgaacat tatcataaat
    24721 atcaattcgc tttgaaaaga aatccgacat ttcttcaacc ggtttacttc tttcttgcgc
    24781 taaccatttt ttgaggtcaa gcatatatct tttgatttct tcaggtgttc ttattttact
    24841 ttccaacata taaaacagtc ctttctttat tcggtctttg ttatatccaa tgaaaatcgc
    24901 tcaaaagcat tttcaagtaa ctgttgtacc atatctaatg gaatgtctat gccattggaa
    24961 ataatttgtg tagctactcc atgagaataa agccaaaagt taataaatag catttctgat
    25021 ttttctatag ataagcctgt aactttttta gcattttcta tagtaattct gttccatttt
    25081 gcatgaataa tatcttgtaa ggatgaaccc ttcattgtca tattcataaa taaggtattg
    25141 aaaatcattt tttcattttg agcaaattca atgtatgcaa gtccttgact taacagacga
    25201 ttttcatcat ttattttact ttccataaaa gaattatagt aattgtctag ttctatttta
    25261 atatcttctt ttatctcatc catatttgtg taaatacgaa aaaggggttt tgtagagcaa
    25321 ttaagggttt ttgctaagtt acgagcattt acgctactta ttccattttc tcttaccaac
    25381 tgaattgcag cgttaataat atcttgtttt gtaataatcg gttttggtgg catcttttca
    25441 tcctcctttc ggtaacttaa gttgcgcaac tggtgttgtt attataacaa aggcatataa
    25501 ctgtgtcaat agacttcaaa gaaaagctac agacatcgag tgtaatatga tttatttcaa
    25561 taaataccat aatatgtgta cttatatctt aaaaaggaga taaacagtaa aatgtagttt
    25621 agttaaattt gaaacttcgt caaagcttct ttgtacgata agacaacgta caaatcattg
    25681 tatgggtaac agagaaagaa ctgcgttgat tgaggatgaa atctcacatg atacgatgta
    25741 attataaaaa aaggagcgtt tcaaagtgat ttttttaatc atcttgagac aactccttat
    25801 attttttatt taaattctat tttttattgc ctgtatagct attttctcca tcataaggtg
    25861 gcatgacctt gaacagcgtg gttttgtaaa gttgggcacc acaaaacaaa aaatcaacaa
    25921 gcccttgtgt ggtctgctga cgtgtttact atactatact ttatccaaaa ccgcaagcac
    25981 tatgggaggc tataaggcgg cggccattca ggggcgatcc cctcgaaacc gggatagggt
    26041 tcccatgtgg gacataataa agagcgcccc ggaggtatca actagcatct ggggcgctct
    26101 ttattatgcc ccgtgggaac catgcccacg aagcagggcg tacaacggac agataggtcc
    26161 taccctcctg acaaactgga atctccgaaa acaatcataa caatcatggc aagattatat
    26221 acactcctgt aaaatatttt tgtgtgtacc atgaagtact gcttgccact attgagaacg
    26281 gataaaaggg aagtacgtat tataccaata aggtgcatga aacaaaatac tcatttgata
    26341 aagttaaata aacctgtcgg aaccccacca atccggcaaa agctcactca atttaacagt
    26401 tttttctgtt tcgacattaa ctaaaatctc aatacaagca ctatccttat caagttgcat
    26461 taaatattca cgacaagcac cacatggtga gccaacactc ccattgctca tcacacaaac
    26521 taatttagta attttacttt cgccatttgt tatcatattg gcgatagcat ttctttctgc
    26581 acacattccc aatgtacagg ctgtatcaat acacacacca gtataaacat ttccgctgtc
    26641 ggtaaggatt gctgcggcaa ccccaccagc atcgataaaa ggtgaaattg tgcgtggatt
    26701 taattttccc ctagccttat tataaagttc cttccatttc atcaggttga tcctcctgtt
    26761 gtatccatat aaataatctt ttatacgcct tttccgccta aggcacacaa cacattactc
    26821 gcttttttca ttctaatatt cgtgcgtgaa aatgtcaaac ggtttacttc gtttcattac
    26881 ttcatactgc tgttcataac cctgtttgtt cacttgggca ccattagagc accaatttac
    26941 ccgtttcagg gttgtagcga acacagcaga aatgctcatt tcatgggctt ttctggctag
    27001 aaaaaaaatt ttatcgctgc cagatattta ctatttttta ttaagttgct ggagttccac
    27061 caccatcagg aggagtagta gagccaccac cattatttcc accattatca ggaggagtag
    27121 tagagccacc accattgttt ccgccattat caggaggagt agcagagcca ccaccattat
    27181 ttccgccatt atcaggagga gtagtagagc caccaccatt gtttccgcca ttatcaggag
    27241 gtgtagaacc gccaccatta tcacctgtgt tattattgtt attattattt tcttctggtt
    27301 ggttattgtc tggttttttt gtatcttcag atgatggttg agaacttact ttagtaggag
    27361 cagtaccttc aataaatgct gctttagagc cagaagattg ctttcctcca tcctctaaat
    27421 taatttttgt aaaatataca tttttaggaa cttcaaattc agtaaccgtt aaattagcat
    27481 gtattttttc cataattttt tcccatagtt ttgcagtact agtacttcca tgtggtactt
    27541 ctctacgttt tatagtgtta ccagaatcat cttttctacc agcatcatct ccaatataag
    27601 ttgcaccaac ataataagga gtgtaaccga caaaccaagc atcatatgca ctatttgtag
    27661 tacctgtttt acctgctaca ggcattccct ttggaagtgc agcaacacca ccagtacctt
    27721 cagtaattac actttctaac atatctgtaa cgacataagc tacttcaggt tctacaactt
    27781 tatgttcctc aggagcattt ttaactaata attgaccatc aaaggtagtt atagtagtaa
    27841 aaatagaagg ttctacataa ataccgtgat ttgccaatgt accataagca gcagccattt
    27901 gaagtggagt aataccattc gccataccac ctagtgtcaa tgatgggaat tttctatcac
    27961 ttgcttcaga tgaacctgag tgattattct ttaaagttgt tataccaaag ttctttaagt
    28021 aatccatcat tatatctaca cattcatcat aagagtctcc aagcatttca gcagttttga
    28081 cagctatcgt gtttgaagat ttagcaagtg ctttacgtaa actcatagaa ccatgaccag
    28141 cagtagtagt tcttggattc catttattat tttcttcaaa cttatatcct cctcgagaat
    28201 cacttagagc agttgcttgt gttattttta gagtatcaat agctggcgta tatattgcta
    28261 aaggttttat agaagaacct ggctgatgag ggttagtagc acgatttaaa gtttttctac
    28321 cacttatatt tcttcctcca gctagtgctc ttatttcacc atttttataa tctaagatta
    28381 ccattgcagc ttgcggttgg ctaactccat caggaccgac catatgtcct gggaaattgc
    28441 tatttctatc aaattcttct tctaaagcat cttgcatttt agggtctata gttgtgttta
    28501 caattagacc accattgtag aataagtttt gagcttcatc atttgtatgt ccttgttcca
    28561 ttaaagcatt tataacttca ctttcgataa ggtcttctac agatgaagaa actttatctg
    28621 atgattgagg caattttatt tctattttag ctttgattgc ttcatcatat tcactttgtt
    28681 ttatatattt caattctaac attttcttta aaacaatttc ttgtcttttt ttagcttcag
    28741 gattacttac agcctttcta acaactaatg tacctgcttt taattgtcta taagtatcat
    28801 tagatataag ctcccaagat tttatttttt caatcatatc aaagtccact tgagtagggt
    28861 catctaaatc atcatcagtt gtgttaacaa aaaataataa tttattctct aaatcctcct
    28921 tagtttcatt tccctctaac ttagaagttt tataggctga aaatcttgat ggatttttag
    28981 ttgaaccagc aagaagtgct gactctgcta gagtaaggtc agctgcagac ttatcaaaat
    29041 aaccacgtgc accagcctca gcacctgcta gacctctacc aacgaagaag ttatttaaat
    29101 aagtttctag tattttatct ttacttactg ttttactcat ctcatgtgca tagtaaatat
    29161 cctttatttt acgaggtata gtttgctgtt cagttgttaa cagcattttg gatacctgca
    29221 taggaatagt actaccacct tgcttagtac ctgtaagggt ctttaatact gacctaaata
    29281 aacctttaat atcgacaccc ttatgctcat aaaaacgctc atcttctatt gcaattacag
    29341 catgttgaag ataaggggaa atcttatcta atggaacggg tgtcttttta tttacacttg
    29401 gagctgtaga taatgttttt ccattagcgt atttaatagt agttgtttgg ttcgtctgtt
    29461 tatctaaaac agcttctgta actggagtta catctcttag tgacgcaaat actagaccag
    29521 cagtacctgc tgtaccaaca actagtaaaa ctaaaaatac tatcccaaat actcttagtt
    29581 ttttaaattt atcgcttttt ttagattttt tcttgttttt tgttgaacga gtcttgttag
    29641 ctgaagttct attaattggt ttatttgtat taccagatga gctaactttt tttctcctga
    29701 ttttatttcc atttttatca ttatcgttat tcataagatt ttaacctcct tacttaatgc
    29761 aatattataa cacaaatagg agtagttttg taattaatag aaaaaatgaa tttgctataa
    29821 tattctgtac attgtatatg tataaacgta atatattaaa aagtctatat ttttatttta
    29881 tttatttttg acaaatatta taaaaattat gccaaagcta catataaata aataggaggg
    29941 gataattttg aatagaatag tagatagaaa aacaaaaaat gaatttagaa aaatcatagc
    30001 tattttttta gtaattcttt tcttgtctgt atttataggc agttttatat atatagataa
    30061 aactttaaga ccgacaataa cagttttagc agagactaaa gctcttgaac tagcaaatag
    30121 gtcgataaat aaagctgttg ctgagatggt agaaggaaaa ataaattatg aagatttaat
    30181 ggatatacaa cttgataata atggtaaaat aactatgata caagcaaaca ctataatgat
    30241 gaatgaaatt gcatctgcaa tagctttaga aatacaagat gaattaaaaa aggataagac
    30301 agcaagttct tacataccta taggaacagc attaggaagt cctatattag ctaaatacgg
    30361 tcctaaactt gaagtatcta ttgaacctat aggaacagta tcagtaaact ttaaaacgga
    30421 atttgagtct tcaggtataa atcaaactag acatagaata tatttagaag ctcaaacaca
    30481 agttaaagta gtaataccac ttataacttc aacaaaacaa attaaagctc aaatacctat
    30541 ttgtgaaact ataatagtag gtgatgttcc agaaagttat gtaaatattc cagaaaaaaa
    30601 tctaggaaat gttttaccta acactggaaa gaataccaac aagtaatata aagttgtata
    30661 tttttaaata aattcaaaat aatagtttat aaataaaatt tttggaaata atattaatat
    30721 aatattaata aaaatttata ttttatacta tgtaaaatat ctaatatatt tggtctgtag
    30781 gttaaataaa agtaccttta gaccaaatat attattaata acttaataac atcttaaaac
    30841 ttatttattt atattattta caacatccat ttaataaata caccataacc tacagctggg
    30901 tcgttttcaa ttgcatgtga cactgctccg attattttat tatcttgaac tattggagta
    30961 ccactcatac cttgtacaat accaccagtt tgagctaaaa gctttttatc tgtaatttta
    31021 attttaaaac ttttactttc tggatgtttt tggttttcta tatctataat ttcaatatta
    31081 tattttttac acttattaaa gttatcttgt aaaattattt gagcttttcc taatttaact
    31141 tcatctaaag atgctacttt atattttttt aacttggaaa tatcaaagtt attaatttga
    31201 cctcttattc caaaatcagt attttctgta aagctaccaa taatattttc tcgttttgca
    31261 tttatacatc caacactacc tttataagat ttttctatat taaggtgctt agttgtagaa
    31321 attgaacctg ttttaatagg tatttttctt gcattaccaa cacttattgg atgacctaca
    31381 gctccaaact ctgatgtatc tggattgata tacgttaaag tagcaaatcc agaaagtaaa
    31441 ttgttaaaac taattttttc taatatgtgt ttggtagttt tcattgttat aagttgtcca
    31501 cctctattta ataaaataga tacttgtttt tcttctggta aagcatttaa ggtattggca
    31561 aagtcactat atccatcaac ttcagcatta tttaccttta ctatagcatc acccaatcta
    31621 aatggagatg cttctccagg atttctaact atgatatttt ttaattctgc gtcaatttga
    31681 agtacgtttc caattggtat taaatattca ggttcatttt tttgtaaatt ttgtgcataa
    31741 attaaattat ttgagaaaaa atataataaa aataaaaatg ttaaaacaat tgtaaatttt
    31801 gacttagcta agttggtttt tttacttaaa gtattatttt taaaatgaaa atttttaagt
    31861 tgcataaaac acctctttct tattaaatta ttttaataat agaattgaag ttttcttgct
    31921 gcttctatat tatgttgtgc aagaatcatg ttttgatgct tggaaaacta gctcaataac
    31981 accttagttt tcataaaaat aaataaaata aatagtgtat atgataaaat aaatacatat
    32041 tcatataaat aaaaataaac aataactgtc tagcataaga aaggagcaaa aaatgaaaaa
    32101 atatagattg ttagttactt gtataattgc catgggaatc ataattatag gaacatcaaa
    32161 tccagttagc aatttttaca agactacaaa tttgattagt tcaggaaata tcacaaatga
    32221 aaaagcagat aaagatgaaa attcaaacaa aaataataaa gataaaaatg aagacaaatc
    32281 taattctaaa agtaataaag ataagtcatc agaggacaaa tcgtcagata ataaaaatga
    32341 aattcaaaaa aacaaaaaac ttttaatttg tatagaccca gggcatcaag gaaaaggaga
    32401 tagcaattta gaaccagtag ctccaggttc ttcctctaaa aaagcaagag tatcttcagg
    32461 aacagagggt attgctacaa aaaagccaga atatgtttta aacttagaag catctcttgt
    32521 tttaaagagt atattagaat ctaaaggtta taatgtaata atgactagag agacacatga
    32581 tgtaaatata agtaattcag aaagagcaat acttgctaat gacaaaaaag cagatatggt
    32641 tgtaagaata catgcagata gtttaaataa ttcttcaaaa actggagcat ctatattgat
    32701 tcctgaaaaa gatggaaaat atacagctcc tatatatgaa gaaagtaata aatgtgctga
    32761 atttatcaaa cagaatatgg aacaatcagg tatacaaatc aatggaatcg ttcaaagagg
    32821 tgatttgact ggattcaact ggtcaaaagt accagctgta ttagtagaaa tgggatttat
    32881 gagtaactat aatgaagacc aaatgatgtc aaatccagac taccaaagaa aaatgatgca
    32941 gtgtatagct gatggattgg atgcgtattt taaataagat atgaatagag aaaaagttag
    33001 gttggtatta ttttcctaat ttttatctct attttttatt gtacataaca caaagaagaa
    33061 gtttatttta ggttaaattg tcgtaaatat aactaaaaat attgggaata cttgatttaa
    33121 gtttaaaaag aatatattat agtatagaat gtgtaaaatt tttttgagag gtgttgaaat
    33181 gctagtagta gaaaatgtga gtcatggatt tggtgcgaga acaatattag aaaatgtatc
    33241 ctttagactt agaaaaggtg aacatattgc actagttggt gctaatggag aaggaaaatc
    33301 gagtttttta aatataataa caaagaagct tatgccagat gctggaaata taaaatggtc
    33361 ttcaagagct acagtaggtt acttagacca acatacggta ttaagcaagg gaaaaactat
    33421 aagagaagtg cttagagagg cttttaagca tatgtttgac ttagagcaag aaatgattgc
    33481 tatgtatgac aaaatgggag aagctagtga tgatgaaatg agtaagcttc ttgaagaaac
    33541 agcagagata caaactatac ttgaaaatag tggtttttat atgatagatg caaagataca
    33601 agaagttgca aatggtcttg gtcttggaga aattggtctt gataaagatg taactgactt
    33661 aagtggaggg caaagaacaa aagtacttct tactaaactt ctacttgaaa atccaactat
    33721 tcttatattg gatgagccta caaactattt agacgaagag catataacat ggcttactaa
    33781 gtatcttcaa gagtatgaga atagttttgt cttagtttct catgatattg aatttataaa
    33841 taatacatgt aatgtaatat atcatatgga aaatggagag ttaaatagat ataaaggaaa
    33901 ttatgatgaa tttgtaagac ttaatgatat aaagaaacgt caagaagaac aagcttatga
    33961 taaacaagtt gaagaaagaa aaagactaga ggattttgta gctagaaata aagctagagt
    34021 agcaactaga ggaatggcta acagtagaca aaagcaactt gataagatgg aaatattaga
    34081 aagaccaaaa gaaaaaataa aacctacttt tgcttttaaa gatgctagag cagctagtaa
    34141 aataattttt gaaacagaag atttagtttt agggtatgat gaagcattga caaaaccact
    34201 taatttccat ttagaaagag gaaaaaagat tgccttaaaa ggaatgaatg gtattggaaa
    34261 atctactcta ttaaaaactt tacttggaat aataaaacca tttgaaggaa atgttaaatt
    34321 aggtgactat ttggaagttg gatattttga acaagagagt tcaagagaaa atagtaatac
    34381 acctatggat gaagtttgga gtgaatttcc agggcttaca aactttgaag ttagacaggc
    34441 tcttgcaaaa tgtggactta caaatgaaca tataacgagt caaatgagag tattaagtgg
    34501 tggagaagct gctaaggtta gactttgcaa agtaatgttg aaaaatataa atttcttagt
    34561 attagatgag cctacaaatc atttagatgt tgaggcaaaa gatgaattga agaaggctat
    34621 caaagagttt aagggaacag ttcttttagt atgtcatgag ccagaatttt attcagaaat
    34681 tgttgatgat gtatggaata tagaagattt tacaactaaa atcgtgtagt gatttaactt
    34741 attgctgggg ggatttcatg aaaaagtttc taaatgtact tttttcaatt tgtattagta
    34801 ttgttataat agtaggtgtt attaatttta ctgttggatt taaacaactt tattattttg
    34861 acattgatta cttaaatata tcagagttat ctggtttatc taaagatgat ataaaattga
    34921 actacgatta tctaattgat tataacttaa ataagaatgt gtctgagttc aaattaccaa
    34981 cattaaaatc ttctccacaa gggaagatac attttgaaga agtcaggaat atatttcaaa
    35041 atataaataa attagctaaa ttattattgg tagtttcttt agtaggaatt atattgagtg
    35101 taaaaaataa aaatataaaa atcttaaaaa ctacatcaat aacacttata atta
//
LOCUS       DAGSHT010000020        32864 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGSHT010000020 DAGSHT010000000
VERSION     DAGSHT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.021"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(194..799)
                     /locus_tag="MJB72_000921"
     CDS             complement(194..799)
                     /locus_tag="MJB72_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY8262046.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(924..1041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1101..2267)
                     /locus_tag="MJB72_000922"
     CDS             complement(1101..2267)
                     /locus_tag="MJB72_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8262047.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2415..4556)
                     /locus_tag="MJB72_000923"
     CDS             complement(2415..4556)
                     /locus_tag="MJB72_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBY8262048.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4700..5807)
                     /gene="prfB"
                     /locus_tag="MJB72_000924"
     CDS             complement(join(4700..5731,5733..5807))
                     /gene="prfB"
                     /locus_tag="MJB72_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBY8262049.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5822..8497)
                     /gene="secA"
                     /locus_tag="MJB72_000925"
     CDS             complement(5822..8497)
                     /gene="secA"
                     /locus_tag="MJB72_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBY8262050.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8629..9180)
                     /gene="raiA"
                     /locus_tag="MJB72_000926"
     CDS             complement(8629..9180)
                     /gene="raiA"
                     /locus_tag="MJB72_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBY8262051.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9733..10407)
                     /locus_tag="MJB72_000927"
     CDS             complement(9733..10407)
                     /locus_tag="MJB72_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBY8262052.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10410..11462)
                     /locus_tag="MJB72_000928"
     CDS             complement(10410..11462)
                     /locus_tag="MJB72_000928"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBY8262053.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11511..12470)
                     /locus_tag="MJB72_000929"
     CDS             complement(11511..12470)
                     /locus_tag="MJB72_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBY8262054.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12563..13084)
                     /locus_tag="MJB72_000930"
     CDS             complement(12563..13084)
                     /locus_tag="MJB72_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBY8262055.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(13095..14429)
                     /gene="celB"
                     /locus_tag="MJB72_000931"
     CDS             complement(13095..14429)
                     /gene="celB"
                     /locus_tag="MJB72_000931"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBY8262056.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14468..14794)
                     /locus_tag="MJB72_000932"
     CDS             complement(14468..14794)
                     /locus_tag="MJB72_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8262057.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14818..15126)
                     /locus_tag="MJB72_000933"
     CDS             complement(14818..15126)
                     /locus_tag="MJB72_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY8262058.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15267..17180)
                     /locus_tag="MJB72_000934"
     CDS             complement(15267..17180)
                     /locus_tag="MJB72_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8262059.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17512..18294)
                     /locus_tag="MJB72_000935"
     CDS             complement(17512..18294)
                     /locus_tag="MJB72_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBY8262060.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18297..20516)
                     /locus_tag="MJB72_000936"
     CDS             complement(18297..20516)
                     /locus_tag="MJB72_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBY8262061.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20780..21877)
                     /locus_tag="MJB72_000937"
     CDS             complement(20780..21877)
                     /locus_tag="MJB72_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262062.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22192..23385)
                     /locus_tag="MJB72_000938"
     CDS             complement(22192..23385)
                     /locus_tag="MJB72_000938"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBY8262063.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23522..23625)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23814..24323
                     /gene="yyaC"
                     /locus_tag="MJB72_000939"
     CDS             23814..24323
                     /gene="yyaC"
                     /locus_tag="MJB72_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBY8262064.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24434..24862)
                     /gene="fabZ"
                     /locus_tag="MJB72_000940"
     CDS             complement(24434..24862)
                     /gene="fabZ"
                     /locus_tag="MJB72_000940"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBY8262065.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24878..25897)
                     /locus_tag="MJB72_000941"
     CDS             complement(24878..25897)
                     /locus_tag="MJB72_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBY8262066.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26094..26354)
                     /gene="spoIIID"
                     /locus_tag="MJB72_000942"
     CDS             complement(26094..26354)
                     /gene="spoIIID"
                     /locus_tag="MJB72_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBY8262067.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26452..27120)
                     /locus_tag="MJB72_000943"
     CDS             complement(26452..27120)
                     /locus_tag="MJB72_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBY8262068.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27186..28250)
                     /gene="spoIID"
                     /locus_tag="MJB72_000944"
     CDS             complement(27186..28250)
                     /gene="spoIID"
                     /locus_tag="MJB72_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBY8262069.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28337..29590)
                     /gene="murA"
                     /locus_tag="MJB72_000945"
     CDS             complement(28337..29590)
                     /gene="murA"
                     /locus_tag="MJB72_000945"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBY8262070.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29611..30348)
                     /locus_tag="MJB72_000946"
     CDS             complement(29611..30348)
                     /locus_tag="MJB72_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBY8262071.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30832..32127)
                     /locus_tag="MJB72_000947"
     CDS             complement(30832..32127)
                     /locus_tag="MJB72_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBY8262072.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32578..>32864)
                     /locus_tag="MJB72_000948"
     CDS             complement(32578..>32864)
                     /locus_tag="MJB72_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY8262073.1"
                     /translation="PVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAA
                     DKIIYIPNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
BASE COUNT         9833 a         6166 c         3419 g        13446 t
ORIGIN      
        1 tttaatagtt aatcgtttta attcttcctt tattttaaaa tttaaatttg tctaaatatt
       61 tcttatttta tatacatttt ttgataaatt aataaagcca taatatattt aatatatcaa
      121 tcacaatata ggactgaagt tataaataaa ttatggctat tcaatacatt taattttata
      181 ttttcttgtc taattatttc tttattatat ttcccatttt tttataaagt ggcaatgtta
      241 ctaaagatat tataacagcc ttcagcacat taaatggagc tatcatccaa attacaaaag
      301 tgaataaatc atgaacttga ggatttatag ctgaacctaa acctattata gcatctagtc
      361 ccataagttg accataaagt ggcaacatta caaaataatt tactaatgaa ccaattatag
      421 tcatagatat tgttcctaat acaaaaccaa tcagtacacc ttttaaatct ccacttctct
      481 tatatgaata acaaagtatc attacataag aaccaccaat taatatattt gcaaattctc
      541 ctattcctcc agttgttgtt gctgttattg cttgtaacac attttttact acaactattc
      601 caaatccaac taatggtcct agagacattc ctccgaatat tgctggaata tctgataagt
      661 caatttttaa gaaatctgga aatatacctg gtattggtgc ttgaataaac attaataaat
      721 aagatattgc agataaaatt gacattacta ctaaagttct tgttgaaatc atattgcctc
      781 tttttgctgt gttttgcata attattttcc ccctaatcaa tatgtttata ttttttagga
      841 aaaacaaaaa actcgaagac cctatcttcg agtttttatg gatatagttt aaacaaacta
      901 aaataaactt atttatcttc cataaatatc ttctctcatc cagactttac tgtcggcttt
      961 ggaatctcac caaatcaacc acctaagtag ttcgcgggct ataccgccgg tagggaattt
     1021 caccctgccc cgaagatctt ctttgatttt ttaattttcc ttacactctt atattagtta
     1081 attttttaat aattgtcaag ttattttttc tcatacacta ctttgccatt tattatagtg
     1141 taaagtacat tactttgaat ctctagtgga gaattatccc aaattactag gtctgcatct
     1201 ttaccaactt ctatagagcc tactctatct tctattccta aagccttagc tggatttatc
     1261 gtaattgatt ctattgcttt ttcttctttc ataccatgtt ttactgcaat acctgcacat
     1321 attggaagat attgaactgg tattactgga tggtctgtca ttatacatac atcaagacct
     1381 gcatttgaaa gtattcctgc tgtattaaat gtcaaatttc taagctcaaa tttagacctt
     1441 tctgacaaag atggccctac tattacagga aacccttctt ccactaattc atctacaata
     1501 agatgacctt ctgtacaatg gtctaatgta agctttaaat taaattcttt tgcaattctt
     1561 attgctgtaa acatatcatc tgctctatga gcatgtgcct taaaaggaat ttctcttctt
     1621 aaaactggta ttaaactttc catttttata tcatattcag gtttttcaca gtcttcatca
     1681 tcatgactct catacatatc aatatcctct aaatattctt ctgctttttt taggttttct
     1741 ctaagtaaag cagctatagc cattctagtt tgaggagatt tatcatcctg accataacag
     1801 ctctttggat tttctccaaa agcaatttta gatgcaactg gattttttat taccatttta
     1861 tcaattcttt ttccacatgt ctttatagct atacattgac cacccataac atttgcactt
     1921 ccaggggttg tacaaacaga agttattcca ccttcaaaag cttctttaaa agttctatcc
     1981 attggattaa ttccatctat aggatttaat tgtggtgtta ttgggtcagt ctcttcattt
     2041 ccatctgctc cttcaaaccc tataccatct tcccataaac ctaagtgagt atgagcatct
     2101 ataaatccag gaaacacaag tttattacta gcatctatta cctctacatc taaaggagct
     2161 atcaaatctt ctccaatttc tattattttt ccttcatcta taagtatatc tccatgaatt
     2221 atcccatttg ttatggtatt tattttacca ttttttataa aaatcattca ttttcctcct
     2281 gattttcaag actatcgttc ttattttaat atattttctt actatatact tttacaaatt
     2341 tttaacattt atacactcga gtaatcttat ttacaaatat agaatctaag tactataacc
     2401 atacataaac ttatctattt tttcattgat aaggcaactc tttttttgtt taagtcaatt
     2461 ccaatgactt ttacatctac tatatcacct actgtaacaa tactcatagg gtctttcaca
     2521 aaactattac tcatttctga tttatgaact aatccgtcat tttttattcc aatatctaca
     2581 aatgcaccaa agtctacaac atttcttatt gtacctttta aagtcatacc ctcttgtata
     2641 tcttcaattt ttaagacatc agttctaagt ataggtttta taccttcttc tcttgggtct
     2701 cttcctggtt ttttgatttc agctatgata tcttttaacg taacttgtcc tacttccagt
     2761 ttttcactta attccctcaa gccaatttct tttatttttt catctatttc acctatattc
     2821 ttattcttta catcatctaa agaatatcca attatctcaa tcattttttt acatatatca
     2881 taactttcag gatgcacacc tgtattatct agtggatttt taccctctaa gattctcata
     2941 aatccagcac attgagtaaa tgcttgaggt cctagtcttt ttactttttt caattgtgct
     3001 cttgacgtaa aatctccatt ttcttctcta tatgctatta tattttttgc tatagcttta
     3061 cttattcctg caacatgttc tagtagggag tatgatgcag tatttaaatc tactccaaca
     3121 ctatttacag aatcttcaac aactccatct agtacttctt ctaatctctt tttgtttaaa
     3181 tcatgctgat attgaccaac accaatactt ttagggtcta tttttacaag ctctgctaat
     3241 gggtcttgta atcttctagc tatagatata gctcctctta ttgatacatt tatatctgga
     3301 tgttcctcat ttgcaagttc agatgctgaa taaacagatg ctcctgcttc actaactatc
     3361 acatactgca cttcgctatc tatttctttt atcatttctg atacaaaagt ttctgattct
     3421 ctagatgcag taccatttcc aattgaaatt atatctatat cgtatttttc aatcaatcct
     3481 tttaaaactt tctttgctcc ctctacatca ttttgagggt ctgttggata tactgttgta
     3541 taatctagta gtttaccatt tttatctact acagctattt tacatccagt tctaaatgca
     3601 gggtcaaatc ccataactac tttgtcttta actggtggtt gaagcaataa acttttaaca
     3661 ttctttccaa atacacttat agctctttct tgagctattt ctgttaaatg atttcttatt
     3721 tctctttcta ttgatgggaa aattaatctt ttataagaat cttctattga acttacgatt
     3781 tcttctttat ttttgaaatt tttatcattt acatattcgt ttattatgta atttaaaact
     3841 ttatcattat ttatttccaa cttaaccttt aggaagcttt ctttttctcc tctgtttatt
     3901 gccaaaactc tatgtggagc catacttttt actgcttcgc tataatcata atacatatca
     3961 taaaccgatt tctcatcagt agcacttttg ctaactatca taccttctct taaagctaat
     4021 tctcttatat actttctaat cttagcatca tctgagacta gttctgcaat tatatctcta
     4081 gcacctttta gagcatcttc aatagaaaga acttctttct cttcatcaag ataattcttt
     4141 gcctctattt ctatattatc taaattattc tctagtattg acaatgctaa attttctaat
     4201 cctttttctt ttgctattgt tgctctagtt cttttctttt gtttatatgg agcatatata
     4261 tcttctactt cttgtaatgt ttttgctttt tctatatttt gtgttatttc atctgttaat
     4321 ttcccttgtt catctattag tctaactaca tcatcttttc tactttgaag atttctcaaa
     4381 tacattaatt tttcataaaa ttctcttagt gtaacatcac tcatttcgcc agtcatttct
     4441 tttctatatc tagctataaa tggtattgta tttccttcat caataagttt taatgtatta
     4501 tttatttgtt catctctaag attaaactct ttttttaaga tttgatttat atccaattta
     4561 ttcactccta atgttagttt ttcataagta taacaaaaca gaacgaaggc tgagggttat
     4621 cgtcctcagc ctaagtgctg taagtgcata ttaagcacta accttcttat acatatatct
     4681 taattacagt gcatatttat taaactattt tattcatttt taagtattca ttaataaaca
     4741 agtctatatt tccattcatt acactatcta catttccaaa ctcagcattt gttctatggt
     4801 ctttaaccaa tttatatggt tgaaaaacat atgacctaat ttgactaccc caagttattt
     4861 gtgagtattt gccttgtata tcttctattt tttccttttg ctctaactct tttaattcta
     4921 tcaatttcgc cattaaaagt ctcatagctc tgtccttatt tagatgctga gacctttcat
     4981 tttggcactg aactactaca ccagtaggga tatgtgttat tctaactgct gaatcagtcg
     5041 tatttacatg ttgccctcca gctccagaag ctctatatgt gtctatcttt aaatctgatg
     5101 gattaatttc tacttctata ttttcatcta actctggtgt tacatctata gatgcaaatg
     5161 aagtatgtct ttttcctgat gggtcaaatg gagatattct cactaatcta tgaactcctt
     5221 tttcactctt taaataccca taagcatttg ttccttctac aagaattgta gctgtcttta
     5281 tccctgcttc agggtctgat attatatcta aaagcttaac tttataaccc ttactttctg
     5341 accatcttat atacattctc aaaagcattt gagcccaatc ttgagcatct aagccaccag
     5401 taccagcatt tatagataat atagcattat ttttatcata ctctccattt agaagagtct
     5461 ttattttcat ttcagataat ttttcttcca ttgattctat ggacttttct atatctcttt
     5521 ctacagaatc atcatcttct tctaaaccta tttcaattaa tacttctata tcttctaata
     5581 agctttttaa gctttcatat tcttctaatg tctcttttaa agatttattt tcttgaagta
     5641 ctctttgagc gacttcatta tcattccaaa aatcttgttg attcatcttt tcttcattaa
     5701 tttgtatatt tttcatcaat gaagctatgt caaagagaaa ccctcaattc ttctaaattt
     5761 gaagccatct gctccatact atgtctatac tcttgtaact ttaacattta atcctccgag
     5821 attatcttcc acaacagttt ttatattttt taccacttcc acaagggcat aagtcatttc
     5881 ttccaacctt ttcatctttt ttaacagttt tacttgttgg aagtgttcca tctgatggag
     5941 aagatagatt ttcaacatct acaactgctt ttctttccac tggagtttct attgttatat
     6001 taaataaata tcttacagta tcttctttga tatgtttatt catttcatca aacatatcaa
     6061 aaccttccat tttataagca ataactgggt cttgctgacc tacagcacga agaccaatac
     6121 cttggcgtaa ttggtccata gcatctatat ggtctatcca atggttatca acagcttgaa
     6181 gtaagattac tctctcaact tctctcattc tctcatagcc aacttgttct tcttttgaag
     6241 tatatatctt catagctatt tcataaactt tttctgttat ttcaactgga ttaagttttt
     6301 ctatttctgg tatttctatg ctaccttttg gcaagaacag attatacata tgttctttga
     6361 atccttcttc atcaaatcct ttatcttgtg tataaagagt aactgcttct tctattatag
     6421 aatgagtcat tgattgtatt tgttcttgta aatcttcacc ttctaataca cgttttctct
     6481 ctgcatatat tatttctctt tgtttattca taacatcatc atattgaagt acatgctttc
     6541 ttattccaaa gtttttacct tcgactttct tttgtgctcc ttcaatagac ttagataata
     6601 ttctatgttc tatcggcatg tcttcttcaa gacctatttt atctacaata ccagatattc
     6661 tatcactacc aaacaatctc ataaggtcat catcaagacc tatatagaat cttgaactcc
     6721 ctgggtcacc ttgacgtcca gcacgacctc tcaactggtt atctattctt cgagattcgt
     6781 gtctttctgt acctattata gcaagtccac ctgcttctac aacttgtttt tgttcttctt
     6841 gtgtttgttg cttaaacttt tcaaatagtt tttcatactc ttctctagct tcaaatagaa
     6901 tttcattacc ttttacaggt ataccttcta ttggagtatc tactctattt actatttctt
     6961 ctttaaatcc atttcttctc atttctctct tagttaaaaa gtctggattt ccacctagaa
     7021 ctatatctgt accacgacca gccatattag tagctattgt aacagctcca agtctaccag
     7081 cttgtgctat tatttctgcc tctttatcat gatgcttagc atttaatacc tcgtgcttta
     7141 tgcccttttt ctttagtatt tgtgataata attctgattt ttctattgat actgtaccta
     7201 caagtattgg ttgattgact ttgtgccttt caattatttc ttgtgctaca gcattaaatt
     7261 tacctatttc acttttgtat acacagtctg gtaagtcttc ccttatcata agcttattag
     7321 taggtacttg gaaaacatcc attttgtaga ttgctttaaa ctcttcttct tctgtttttg
     7381 ctgtaccagt catcccagaa agttttttgt acattctaaa gtaattttgg aatgttacag
     7441 tagctagtgt tttagattct ctttgtattt taagaccttc tttagcctct attgcttgat
     7501 gtaaaccttc tgaatatctt ctaccaaaca taagtctacc tgtgaactca tctactatta
     7561 ctatttctcc atctttagca acataatcaa catctttctt cattattaca tgtgctctta
     7621 aagcttgatt tatatgatgg tataattctg tatgagatat atctgttata ttatcaacat
     7681 taaaatacac ttctgctttt tgtatacccg atgctgttag agatacagcc ttatcttttt
     7741 cttctaattc ataatcatct ggttttaagg ttaaaacaaa tgtgtttgca tctgaatata
     7801 agtgagttga tttgtctcca ggtccagata tgataagtgg tgttctagcc tcatctatta
     7861 gtatagagtc aacctcatcc actatagcat aatttaatcc tctttgaact ctttgctctt
     7921 tgtgtattac catattgtcc tttagatagt caaatccata ttcattattt gtaccataag
     7981 ttatatcaca atcatattgt tcttttctaa cttttggatt ttgaccgtgt ataataacgc
     8041 ctacactcat tcctaaaaac tcatagattt ttgccatttg gtctctatca cgttttgcaa
     8101 ggtaatcatt tactgttaca acatgtacac cttttccagt aagcgcattc aaataaacag
     8161 gagcagttgc aaccaaagtt ttaccttcac ctgttttcat ttctgctatt cttccttgat
     8221 gtagtactat acctccaatc atttgaactc tgtagtgtct aagtcctaac actctcttag
     8281 aaacttctct aactactgca aaagcttctg gaagtatatc atctatactt tcaccatttg
     8341 ctaatctttc tttaaatata tttgtcatat ttttaagttc cgagtctgcc atagattcaa
     8401 atttaggttc taatgaatct attatatcaa cagttttatt gaactttttt aattcttttt
     8461 tatctgccat gttgaataaa ttatccataa agctcatatt ttacatctct cctcgttctt
     8521 tattgtacta ttattcattt taccataaaa actatacttt taatattaat ttaaaaagct
     8581 atcgataatt tatttatcga tagctaatat gattaaatct aaattgcttt attcatgttc
     8641 tattatacca tatccaccat tattacgctt ataaactact gctatttcat ctgaatctat
     8701 gttcttgaac atataaaaat catgtcctat taattccatc tgtaatacag cttcttcttc
     8761 actcattggc ttaacactaa atttcttacg tctttgaatt actaattctt gattttcatc
     8821 cccataattc tcttgttcat cagctagatt tatctctgca tcttggaatc ttatactctt
     8881 gttgtcttta tgtttatctt gtaatctctt tttatatttt tttaattgtt tacttaattt
     8941 atcatataca acatctatag ctgtgtataa attttcctgc atatcttctg ctctaattat
     9001 aggaccattt acagtagcta ttgttacttc tattttttgt cttgcttttc tcgcacttac
     9061 tgtaactttt acatcagtgt ctggatgaat gtaattatct aacttactta gtttagtttc
     9121 gattttattt tttattgcat cagttaattc gatttgtttt ccagatataa ttatgttcat
     9181 aaatacatct cccttcaatt atgccagtat aagaaaattt atactttaat aatatattct
     9241 acacgaactc tcataatcct ctttttatta tttactttta tgattttttc tattcaattg
     9301 atagaattat ttatcatttt tagttacatt gatacttatg tggattaata ttattacaaa
     9361 cacctgtgga taatgtggat aaaactgtta ataactcttg tttttgttta tttaaagcta
     9421 tttttactcc tatttctatc cacaaaaatc tccattaatt gtttattaat atgtgaattc
     9481 atgtcataag atggtaattt taggaaatta tgtgtattat atgccaatat acctttataa
     9541 ttttttaata aatataaacc ctctaaaacc tccataatct atcttttctt tcattattta
     9601 tatttaaaac taattaaaag ttataaagca tactcaacaa taatttcata tttaatacct
     9661 attaacattc atatacccaa attataatta acttactctc tttatttaaa ctaagtattt
     9721 tatattcata tactatcttg aaattatatt tacgagttgt ttaagctttt gtctatctat
     9781 atcttcaaaa ggactgttat ttattctaat cgctgtacca aaatgaaaat tacttgcctt
     9841 agttaattcc tttatctttt ctatattatt aaaattaaga cctccaccta aaagaatttt
     9901 tatgtgatta gaactaagca tcatattctt tatcatttgt atgttatcaa ctatacttcc
     9961 tttccctcct gatgttaata tattcgtaat tttatcataa tccttcaaaa ttttaacact
    10021 atctattgta tttgattcat ctattgctct atgaaatgta acatcaagat tatcacaaca
    10081 tttcaataaa acatttagat ttttctcatc tatactattg tttttatcca atacaccaaa
    10141 tacaactcca ttagcaccaa tctctttaac cacacttata tctttttgca ttatacttat
    10201 atcttcttta ctgtatacaa aacttttcgc atgatgtctt atcattacgt tcacaggtat
    10261 ctttacactg ttaacaacac tttctataag accaaaactt ggtgtcaatc caccttcagt
    10321 taaagcacta actaactcta ttctatctgc accacaatct tctataattt tagcgtcttc
    10381 tactgacatt cctattattt ctaacataac tactcctttg ctaattcata tattgcattt
    10441 gcaaatatct ttgcatttaa tattatatct tctatatcca tgtattcatc aggattatga
    10501 gctattcctt tttgtcctgg aaatgaaggt ccaaacggaa ctatatttgg cattactttt
    10561 gcatacgtac ctccattagt tgttacggga gttccatcta atgatgtaac cctttcataa
    10621 gctagctgaa gctttttaac aagattactc tctttatcaa aatatacagg attaaaatta
    10681 gaaattacct ctatatcgat actatttgga aaactagctt ttatttcatc caatacatct
    10741 ttcaatttat aactaaaatc gtattttacc tttatatcta atatagtatc actattttct
    10801 atattcagct ctttatatgt aacaagcaca ttttcagaca tattttctat aaaacctagg
    10861 tatacatctt cttcataatt gattttactt tttattctaa tctttgccat gcctttttca
    10921 ccatatacaa caggatattt acaatctggt gtaaatccca taataggtgc tttttctttt
    10981 tctaaataat atggtatatc ttcaaatcca ctttcttcat ttgtaccaaa tattattcta
    11041 actctcttat ctaattttaa gtttaattct ttaatagcat ataatccata taaggcagac
    11101 attataggtc ctttattgtc tagtactcct cgtccataaa ttcttccatt agtttcttct
    11161 cccttataag gctgatgctt ccaaccatct ccttcatgta caacatctac atgtccaatt
    11221 acacaaacat aatcatcact atctccatgc tctgcataac caatataatt atccaagttt
    11281 ctaaccttaa atcccaattt ttctgatatt tctaatgctt tattaagtgc ctcccctact
    11341 ttttcgccaa aagggaagcc attttctgac tcccctttga cactaggtat ttttacaata
    11401 tctataatat cacttaataa atcttctttt aactcatcta ttttattact tatgatattc
    11461 ataaaaatct ccttattata aaactctatt ttaattatgt aaccactact ttactcactt
    11521 acttctattg gtgcttttat tctagcctca tatgcttcca tccactcatt tgatataacc
    11581 ttatgtatag tctttccatc tactctattt gaaatatata cagtaggaga ttcttcttta
    11641 gttactactg aaaaagtaaa atcagctata tttgtagcta ctccccattc acctttatta
    11701 ttcatggcaa ctaatgataa atcaccagct tttcctcttc ttttgataag ctgtttatca
    11761 agttcattta ctgcaatatc acatgcttct tgtggatgct tcccttcttt cataagtcta
    11821 actatctcat aagatataca tcccttcatt aaatcttctc ccaaaccagt agctgtagct
    11881 cctcctatac tactatcaac atagaatcct gaaccagata gtgcagaatc tcctattcta
    11941 cctcttttct tcataaacaa tccacttgtt gatgtagctg cacacatttt tccagttttg
    12001 tctaaagata tcataccaac agtatcatga ccagcatatg ggcttaatcc tttgtctaaa
    12061 gtctcttttt ttctcttttt ataatgtaac ttcgctctat cagtcaacat attttttctt
    12121 tcaaatccat ttttatgagc ataatcttca gcacctattc caactaaaaa attatttacc
    12181 ttctcataac ttaacttcct agctatgctg acaggattag aataatctct tataccagca
    12241 actgcgccaa tatctaatgt gttaccatcc ataaatgcag cgtcaagttc tacctcacaa
    12301 ttttcatttg gaagtccacc atatccaacc gatttataga atggatagtc ttcaactctt
    12361 cttacagcaa gctctacagc atcacctgct tccatgcctt tttctaaatg ttctgatgcc
    12421 tcagttattc cttctagggc cattctccat gtcgctatca ttccccacat gttaaaattc
    12481 ccctccttaa attatataga ttttactaat gtatgcttat tataaatagt ataatattta
    12541 aacttgctaa taattaatac tcttattgat aacaaaactc ttctttcatc aaagttatat
    12601 cttctttatt tgctattgct tttgccaatt tacatatgtt atttaatgaa tcattaagac
    12661 caattccacc tttttttgga gtcttaacta tacatatgtc atttttatat tcgtttatca
    12721 catctatatt ttcttctgac atagctgcaa aagctggtat atctgtttta tcatttatat
    12781 ttttagacag tattgctgac atcttagaaa agttttcgta attaaagctt ggtccacaga
    12841 ttactacatc agggtttaat ttttttacca tagctatcat cttactcgtt acttcttctt
    12901 gattatttac aaaaaattca tccccacaaa ataaggtagc tattacctta gattcattta
    12961 aaaattgctc catcattaca cctggtccta atgggcttga ttttccagca ggaggtatat
    13021 tacttttctc ttttcctcca gctccagctt gtatttggtc caatatcata actattttct
    13081 tcataatctt cctcctataa tactaaatca tcaaaagata tatcatcatc ttcctcttca
    13141 acagtttcat tttcttctct caagtattgc ttatctagca tttttataaa tggtatatac
    13201 ataaatattc ctagtataag aagtactaat tgtaagactg caccttgcca tcctgttgtt
    13261 aaaaaaccag agaatataat tggagttgtc cacggtaact gaactccatt tgtaagaggc
    13321 actaatcctg ctgacataca gaagtaagct attattatat ttattgttgg aactattgca
    13381 aatggtatca atagtattgg gttaagcatt attggtagac caaatatcaa tggctcattt
    13441 ataccaaata ttcctggtaa aattgatagt tttcccattg actttatacg tttactttta
    13501 catattaata acatagctat gactaatgat agtgtactac ctgctccacc aaatgtcgca
    13561 aacatatctt ggaattgacc tgttattatg tttggtattt ttgaaccagc ttgaaaagca
    13621 gctaagtttt cagctgatag aatttttaat attggattat acacagcccc aacaacacta
    13681 cttccattta ttccaaagaa ccagaataaa tgtaagaata tataagcgat tgccatagca
    13741 cctaaactat ttcctaattt taataatggc atttgtaaaa acttaaatat aaattcatgt
    13801 atacttccat atggtgtagc ttcaaaagct atcttaacta aaaagaaaac taccataact
    13861 attgcacttg gtattaatgc tgcaaatgat tttgatacag ttggaggaac tccatctggc
    13921 attttcaatg tccatccttt ttttacaact gcagcaaata attttacaga ggttattgct
    13981 gttatcatac ctacaaacat tcccttagaa cccatccaac ctagtggtat acctgttacc
    14041 atgtaaactg attctgttcc ttctggtgta tatggtatta caaatggtgt tattataaaa
    14101 aaggcaacta ttgcaactgc tgctgatgct attgggtcta catctatttg ctttgcataa
    14161 gaatatccaa tccctaaaac tgcaagtaga gtcattactt caaatgttgc tacagttggt
    14221 tgttgcaatt ttacagccca atctggacca aaaatttgac ctacaaattc attccatcct
    14281 tttataggaa aatttgctac taataaaaat aatgaaccta ttattaataa tggtgatgat
    14341 actaaaaaac catctctaat tgcaattaat aatttatttt tacctatttt ttcagccaat
    14401 ggcataagta ttctttccaa tctattcatc tatccaccct catttcctat tattttttac
    14461 tataattcta cgcttgttta gattttatta gctttatagc aagttttaat actttttccc
    14521 cattcatagt tccataatca acttcattga ttatatctat aagttttcct tgagcttcaa
    14581 actttggttt taactccttt agcatgtatt taacttgtgg tcctaataat atacaatctg
    14641 gatttttttc ttcaactatt ttgtcaattt ctgctatcgg aaatgcctct acttctattg
    14701 gtaaattgtg ttcatctgct accttctgca ttttatctgc aagtaaactt gttgacatac
    14761 caaagctaca aaataaatat acttttcttt tcatttttta aatccccctt aattttttta
    14821 ttttaatttt tgaatttctt ttcttaacat aatcatttct tttataaggt ttctctcact
    14881 catacaagtc attaaatggt cttgagagtg aatcaataaa atagatatat ctgatttatc
    14941 tcccccagct tccttttgta taagttcagt ttgtacttta tgtgctttta aaatttcttc
    15001 atttgcattt tcaattaatt catctgcttt tttaaaatcg ttttcctttg catagtcaag
    15061 tgcttcataa agcatagatt ttgcatttcc agcatgaatt ataatcttta aaactatttc
    15121 ttccattttt tcctcctaag ttgcttctct acttttagat tatttatacc ttacaaacat
    15181 ataatatctt ataaaaacaa aaaaacatgc acaacttttt ccatcacatg gaaaattttg
    15241 tgcttatcca tagttcacta tgaattttat ataagaatat cttttacctt ttcaatgtca
    15301 catttactta gaatatctag catattttca tcctctgtta aattagatat ttctttaaat
    15361 aattctctaa atacttttct atcatcatct tttttatacg ctaacaaaag tataatcttt
    15421 atatcctttc catttctaat ttttattggt gtttttaacc ttgcaaaaca taaaacagat
    15481 tgttttatat atttcatgtc tccatgaggg aataatattc cttttccaac attggttgga
    15541 tatgactttt ctcttgttat aatagattgt aaatactctt tttctgcata atgtttttca
    15601 attaataaat tagttaaata tgttatagct tcataaatat catcatgttc taactctctt
    15661 attaaataat cagacatcat taatctgttc ataagtttat atgaagtgtc tttttctttt
    15721 attttgtttg tgaactcttt aagtttattc tcatcctcat cagtaaataa tggtgttgtt
    15781 ataaaaattg gtttttggat attttcatac tcttgaaatg caattataaa atctatagtc
    15841 ttattaaagt tatctctttt taaatcttcc tctcttaatg tttcaataat ttttatgtta
    15901 tcaaatctct tttctatctt tacttttaat agcatactta ctccaaaact aaatggacat
    15961 acaatacttg cctttttatt tgattccctt ttgctgtatt ttctttctag agaggtttga
    16021 aagtgaagtg taagataacc aatttcatct tccgttattt gttttccttt taattcaatt
    16081 tctattgaac ttgatattac tgtaaataaa aaacaaaatt tagtttttat gttatctaaa
    16141 agtggatttt ctaagtaaac attgcttttc atttgatgca ttgtaacatt caaatgacat
    16201 attaattgtt cataaagtaa tatatcatta ttaaaattta ttcctgattc ttctgatact
    16261 aaatttataa ttttttttgt ataacttgct aattcttcat ttagatataa agatttctta
    16321 atattattac tatttatttg tttgttcata cccaatatta aagattctat aaacaatttt
    16381 tcttcataat ttaatactat ggataaatta gatttcaatt catcttctat atccaatata
    16441 agcattttta gcttaagctc tttcttagta tttgatactt ttaaagaatt tcccagcctt
    16501 attcgtatta tatttataag caaaaatagc atcaattgct tgaatgatat atctgtaagt
    16561 cttatatcca tatcctcttc tatagtaagc attatgtttt ttacaattat tatatctact
    16621 atatggaagt actctatttc atctatgttt aatctgtatt tatctatata tttaaataag
    16681 aatgatacta acattattct tatgtctttc tcatcccctt caatatatat accactgcct
    16741 tttttagtcg agatatttaa attataatta tttaatgcta ctgataattt acttatttct
    16801 tctctgacaa tatttttatt tatgtacaac ttattagata aatcatttaa agtaactttt
    16861 ttattattac aaagtagtaa atttagtatc tcaacttgct tatgaagctc actatttact
    16921 cttttaactt cacctcgcaa cttctgattt accagtattt tgtctgttcc atctccacta
    16981 aatttaatcc caatattagg ttttcttttg atataagcat tacaaaaagt agataaccat
    17041 tcatcaatat atttgcaatc atttcttatt gtcctttctg aacactcaaa atcattggca
    17101 atcttattaa catttataaa ctcttctttt aatagcaatg ttgttaataa attttcttgt
    17161 cttttattca aacatatcac ctctttatga ctcaacctaa atttattata tataaaaaag
    17221 agctatacat aaaggtatgc tagctctctt ttaatatgat atttagctga cctggttttt
    17281 gaccccacac tcgcctactg gagggttcgc ttgggttcgc cgcactacta ctctctctcg
    17341 attttattat cggtaggtct tacttctaag ccgcaccatg atcattaacc caatttttag
    17401 tgttaactta cgtggatccc tttgcctgcg actggcttgg actttcaacc acagacctaa
    17461 atatcactta ttcattttaa tattttaaac taaaattgtc aatatataag attattccat
    17521 aatataatta tcatttgctt ttgtaagtag agtcaataca aaaaccttat tagcacctga
    17581 taactttaaa agtttagaaa tttcgtttgt tgttagacca gtagtaaata tatcatctat
    17641 taaaagtata tttttattat ttattaattt aacattttct ttaacaacaa atacattttt
    17701 taattcttct tttcgttctt tcttattcaa tttatatagc attcttgtat atttttttct
    17761 acttatgcaa tctaataatg gaatatttac tattttactt aaattaaatg ctattttttg
    17821 tgcctgatta aaacctcttt tatttagcct ttttttatga agtggaacaa ataatatata
    17881 atcaaatttt atgttttcta aatataattt ttctttcatt atttgagcta tatacttagc
    17941 cataaaggtt ttttggttat acttcagccc taacaccatc ttttttgtca catcattgta
    18001 ttcaatacaa gatattgatt tatcaaaata aaagtcttta ttaaaacaat aactacactc
    18061 ttcaataaat tctttttcaa ttgaatgacg tattattggt tttccacact tcatgcaacc
    18121 atcttgtata aaattcattt ctttaaaaca acttttacat attgaataag tatttgtttt
    18181 ctttatggat ttatcacaaa ttatgcaact tatattctca ggatatataa aatccaaaca
    18241 tttattaatc atatttttaa aattcttaat aaaattatca ttgcatattt tcatatttat
    18301 tctatcaaca acccttcttg tttaaattta tttagcttat atcctaaatt tgaatacctt
    18361 tgattaactc tattattttt tatcatatat tcaagatatt tcacatctcc aactaataca
    18421 actaattttt ttgctcttgt tactgctgta tataataaat tacggctaag taacatagga
    18481 ggagcccacg ctattggcaa tactacaact ggaaattcac ttccctggct tttatgtatt
    18541 gttgtacaaa aactatgatc aatttcatct aattcatcat ataggtatga tacaatttta
    18601 gtctggtcaa ataatatata aatagttttc ttatctttat ctatgtgata aacatatcct
    18661 atatctccat tatagatacc ttctccactt tctttttggt cttctgtttc ccattttttt
    18721 gtataattat tttttacttg cattacctta tcgccaactc taaataatct tttagaaaaa
    18781 ttttcttcaa ctttaaattt ttcttttttg ttaagatact tctgtaactc tatatttaga
    18841 ttagtaactc ccaattcacc ttttctcatt gaagataaaa cttgtatatc ttttaattta
    18901 tcaactttat aaaatttagg taatctttca ttaaccaatc ctattatttc atttaaaatt
    18961 tcttcattag ttgattttct tataaaaaag aaatctttac cttttgtatt tagataaagt
    19021 ggttctccat tgtttatctt atgggcattt acaattatca tactttcttg cgcttgtcta
    19081 aatatttcat ttaatttaac tacatttatg atattagaat taatcatatc cttaagtaca
    19141 tttccagcac ctacagaagg taattggtca ctatctccaa ctaaaattac tcttgttcct
    19201 aactttatag ctctaagtaa attgtacatt aagataatgt ctaccattga tacctcatca
    19261 acaattatta catctgaatt tattgggtct tcttcatcct taaaaaaagt taaatcatca
    19321 tctgttgaaa atcccatttc tagcaaccta tgtatagttt ttgcttcttt attagaagtt
    19381 tcactcattc tctttgctgc tcttcccgtt ggagctgcca gagtcacact tttaccatta
    19441 ttttcaaata tatctatgat agcattaatg gtagttgttt taccagttcc tggtccacca
    19501 gttataatta atactccact attaacagat tctttgactg ctaaaatttg tttttctgct
    19561 aacttaattt tcttatcttc ttctaaaaca ttaatctcgt tatctatatc tatttttaag
    19621 tcttcaaatt catactgtga taattttact atttgactac atactccatt ttcagctaaa
    19681 tagtatggca taaggtatat aagattttca ttgtttactt tctctatatg tattttttga
    19741 ttataaacta gcatcattac acactctttt attatttctc catttagctc caataatttt
    19801 tccgaatctt gtattagaac atgctctggt aaatatgtat gcccattact taaagattta
    19861 tttaaagtgt ataagattcc ctgacaaatt ctatctttag agtttttatc tataccaatt
    19921 ttacttgcta tactatcagc tactttaaat ccaattcctc ttatatcttc agcaagttgg
    19981 tatgggtttt tatttataac ttctattgca ctgcttttat attttttata tatttttagg
    20041 cagtaatttg gtgttattcc aaaaggagat agctgtatta ttatatttct caattctctg
    20101 tcttcttcat aactttttac tatttgtttt acttttttac tccctatacc ttctacttct
    20161 tgtagttttt ctggtgaatt ttgtatcacc tctaacgtat ctacaccaaa cttatcaatt
    20221 attctttttg ccattttttc accaatacca tgtatcattc cagaagataa gtaaacataa
    20281 attccttcta aggatgatgg tgttactggc ataaaactat tcacttcaaa ttgagtccca
    20341 taaatcttat gatttaccca ctttccttca acttctatgc tttctccaat tgctaaagta
    20401 ggcatacatc ctacaaccac aatttcatca ttttcattta ccaggtgagc tattgtataa
    20461 ccattatctt cgtttttaaa gactatttca cttatcattc cttctaattt ttccattttt
    20521 atctcctaaa aatactaata atcgctccat ttaaatgtta gtataatttt atatattaaa
    20581 actatgataa tcgtagaaaa gttttatatt tttatactta acgtaatttc caactattat
    20641 acattttatc agaaacaaaa aaaatctgca tccataaaat atggtaccat gaaatacagg
    20701 taccatattt aaatagctat tttattttaa ttactctata tttagagtat aaattaaatt
    20761 tcataagtat attacagtac taacttattt cagatttatt ctccttgtac ccctcagttt
    20821 tagtaattct tctataaagc atacatagta aaattaaaat tcccatgtaa cctgtatatg
    20881 gatatagtgt acctactaac ttatcaaatg gcagtaaacc tcctatacaa gctaatatac
    20941 ttaccactat tgttataatt ttaaacttag ggtggtcatc ttctacaaat cggtttgtta
    21001 ctgaccataa caatggtaca gctgtagtgt atattcctag taaaagtact actgagaata
    21061 aaatacctat tattggagat atcttatcag ctaagtaaag tgctggtatc tcttttgtgt
    21121 atatatttcc tatgtctgaa agtaatgcca aattcatcat tatagctgct gccataagtg
    21181 ctactccacc tactatacca ccccatacag catctttctt atttactgca gaagctccca
    21241 ttcctgtcaa gaatgttatt actattatta cattgtaaca agtataaagt actcccgaga
    21301 aatatccatt tgatgtagca gttttaatat ttaaactatc aaccatattt ccagcatttg
    21361 ataaagcatc aatattgcta aataaactaa ttgctcctac taaaagtgta aatattatta
    21421 ttataggtcc tatattacct agtattttag aaagccttgt caaacctaat gaaacagtta
    21481 ttagggaaac gattgccata ccaatcctac ctacatatgg atttaatcca tagtactctg
    21541 ataatgtagc tcctgctcct gcaatcatta ttactaatgt tccaaataaa aacaatggac
    21601 caaaccattc aaaaaatgtt ccaagatatt ttccacaata tacttgataa atttttattg
    21661 gctccttaag tttcaattct cttcccttat ctataacttc tgctccaaac catgaaaata
    21721 gtaccataga gattattcca cctataatac ccgcatatcc ataaaacgta aaaaattgca
    21781 gtatttcttg accagttgca aatccagaac ctattacagt agcaacataa gctccagcaa
    21841 aacttataac cgttttaata tttactttgc ctgacataca ctactccccc caattaaaat
    21901 tatttttaaa gtgtaagttt attaagatta tattagactc attattgtga tatttcaata
    21961 tatttttaat tatttaacat tagttaacac attcttaacc atcctttaac atatatccca
    22021 taatatgtat aaatttagta gagtattata taaaatgtat tgaccagatt aaatactgtt
    22081 tattcattat atgatgataa attaacattt attaaaaata tttaactcat aataaaaaag
    22141 ctgttagaaa gtaatcgtaa aattacattc taacagcttt ttagttaaaa attattcacc
    22201 taaagcatct tttcttaact gctcaacctt gtcagttctt tcccaaggta aatctatatc
    22261 agttctacca aagtgaccat aagcagcaac ctttttataa agaggctttc ttaaatctaa
    22321 gtctcttatt atcgctcccg gtcttaaatc aaagtgttta tttactaatt caacaagttt
    22381 ttcttctgaa accttgcctg tcccaaaagt atctataaat atagataatg gtctagctat
    22441 acctatagca tatgcaagtt ctatctcaca tttgtctgca agaccagccg caacaatatt
    22501 ttttgcaaca tatctagctg cgtaagcagc agatctatca acttttgtag ggtcttttcc
    22561 tgagaaagct cctccaccat gtctagaata tccaccataa gtatctatta ttatttttct
    22621 tcctgtaaga cctgtatctc cttgtggtcc tcctataacg aatcttcctg ttggatttat
    22681 ataaatttta gtctcttcat caagtaattc tgctggtata acttccttaa taacatgatt
    22741 aattaaatct tctcttattt tatcatttga tactgtttca caatgttgag ctgatataag
    22801 aactgtatgt actcttacag ctttacttcc ttcatattca acagtaactt gagtttttcc
    22861 atctggtctt aaataatcaa ctaaacctgt ttttctaact tctgtaagtc ttcttgataa
    22921 tttatgtgcc aaagatatcg gaagtggcat taattctgga gtctcattac atgcaaatcc
    22981 aaacattata ccttggtctc cagctccaac tttttctatt tcttcttcta tctcttctcc
    23041 agtcttactt tctagaccct catcaactcc catagctata tctccagatt gttcatcaat
    23101 agaagttata actgcgcaag tatcacaatc aaatccatat tttgctcttg tatatccaat
    23161 ttctcttact gtttctctta ctaatttagg aatgtctaca taagcagatg tacttatttc
    23221 tcctgctact aaaactaaac cagtagtaac tgtagtttca caagctactc tagattgtgg
    23281 gtctttttct aataatgcat ctaatattga atctgatatt tggtcacata tcttatcagg
    23341 atgtccttct gtaactgatt ctgacgtaaa taaatgtctt gccattttta ttccccctta
    23401 tttattctta atttttatta taggttttac cttgttttta attttagaaa tttactcaaa
    23461 ataaaaaaac ctctccttta taagaagagg ttaataatcg taattacata ctaattaact
    23521 gacctcatct ttcaagactt ttgtcttgta ggaattagca ccttttccca tttgggtggt
    23581 tgctgggctt cacagggcct atccctcagc ctctcttgat aaggtaatca agatttaagt
    23641 tttgttttac tttactagct tactattttt ttaaaatttt gtcaatattt ttagtcatat
    23701 tttttctatt cctttttttg taagacaaat aaaattttct atctatctga aatttaaagt
    23761 taggtctttt taatgctttt tattcatata tttttttgaa gggggtgttc ctattgtatt
    23821 tagctaaagt aaactacagc gatgatgatg ttatacagat gctaagttca gttctaaaaa
    23881 ctataattaa tgaaaatacg attgttgttt gcataggaac agacagggct ataggtgata
    23941 ctttaggccc tttagttgga acaatactta agaacagtaa ctttaaatac cccgtttacg
    24001 gtacactaga taaccctatt cacgccttaa atatatatga gtctttagat actataaaaa
    24061 atacacatat acaaggtaac ttcttggcta tagatgcttg cttggggtcg cagagtaaca
    24121 taggtaatat acagattaga gaaggaccta tcctgcccgg aaaaggtgtt ggtaaaaaac
    24181 tacctcaaat aggaaattat tctatagtag gaattgtaga taaaattgat gaaaataata
    24241 aactttcatt taataatata cgccttagct ttatattaga tttagctgaa acaatagcct
    24301 tggctttatt agtatccacc taatctaaat ataaactaaa tgataaaatt ctttatataa
    24361 aaaggtagat attttagagt atctgattaa atactccaaa ttatctacct ttttactttt
    24421 atatttcaag tttttatttt tctactaaag caaacattaa ttcaccttta cagacaagtt
    24481 cttcacctac atatgcctta gcatctgcct tagctatatt ttttctaaga gttgtcattt
    24541 caacttccat agttaatgta tctcctggtc ttacttctct cttgattcta actttatcta
    24601 tacctgcaaa tacacctagt tttcctttgt tttcctctac agacatcatt gccactccac
    24661 atacttgtgc tagagcttct actattagta ctcctggcat caaaggatat tctggaaagt
    24721 gaccttggaa aaatggttca ttaactgtta tattctttat accaacagct cttttcccaa
    24781 cttctagctc tgtaatcttg tctactaata aaaatggata tctatgtggt attaattttt
    24841 ttatttcatc tatatttaac attctaaact ctcctaatta ttttcttttg aaagacccac
    24901 cagtttcaag taagtctatt gaacctaaag ctattcctgt accttttgca acacaagata
    24961 atggttcatc tgctattgta acagttattc ctgtcctctg ttctattatt ttatctaatc
    25021 catataataa tgaacctcct ccagtcatta ttataccgac atctcctata tctgctgcta
    25081 actcaggagg cgttttttcc aaaacagcat gagtagcaac tactatttgc tcaacacatt
    25141 ctctaagtgc ttccaacatt tctgttgaat ttatagttat agaactagga agtcctgtaa
    25201 ttaaatttct tcctgttatc ttcatatatt tttcttcttc tctcttaaat gcagaaccta
    25261 tttcaaactt gattttctct gctgttcttt ctcctataag aagattgtgt tgttttttca
    25321 tatatttaac tatagcatca tcaaatctat ctccaccaac ctttatagat tcacttacaa
    25381 cagctcctcc tagagatatt acagctatat ctacagtacc tccaccaata tctataatca
    25441 tattaccatt aggctgtgat atgtctacac cagctcctat ggcagctgct attggttctt
    25501 ctatgatata tacttctcta gctccagctt gtctcgtagc atcttctact gctctttttt
    25561 ccacttctgt gacacctgtt ggtacgcata ccataattct tggcataaaa aatcttccaa
    25621 atcccttttt atcaactatc ttatctataa aagattttaa catcttctca gttacatcat
    25681 aatctgaaat tacaccatct cttaaaggtc ttatagcgac tatattacct ggtgttctcc
    25741 ctatcatctt tttagcttct tctcccacag ctaaaacaga atctgttctt ttgtctatcg
    25801 ccacaacaga tggttcttct aaaacgattc ccttaccact aacataaaca agcacatttg
    25861 cagtacctaa atctatgcct atatcagctc cagccatttt ttcaactcct ttatttataa
    25921 tattagatta tattataagc ctattttaat actcctataa taaagcatat acaaaaatct
    25981 cgctttaatc aagagaaaat aaatcttttg agagtattat tattttgcta tttataatta
    26041 cttaatatat ttctttttat aatttaattc aataaaaaaa gtaggttttt aacctacatt
    26101 tttttatgtt ccttctcata ttttaatttc gttgctaatc cccctctaat atgtctctct
    26161 gatttatttt tgtccagcac atttttaact tctttggcaa gggatggatt tatttccttc
    26221 aatctttctg taacatcctt atgaattgta cttttactta ctccaaatgt cttggcggtt
    26281 tgtcttactg tagtgttttt ttcaagtata tattttgcta ccactatggc cctttcctct
    26341 atatgagatc tcaaagatta cccccctcac ataatagctt taatgatata tatgtaaaaa
    26401 caccaaaaaa tataacaagc ttaaccaaat atgattaaac ttgttatact attacttaat
    26461 tagactcatt gggtctatag atttttctcc actatacgcc tctaaatgca catgtatacc
    26521 ttcttcactt tctatttgag aagtactacc tacagtacct agagattgtc cttctgttac
    26581 cttctgttct ttcttaacac taacattttt atctagattt gaatatacta ctacaatgtt
    26641 attgtcactt tttattttga ctgattgtcc atattcttca tcatcaaata catctactac
    26701 tattccgttt aatagagatt ttacttcttt gcctttagta caactaatat ctacaccttt
    26761 atgagtttcc caaacatcaa gtgttttaga ataactaggc tctttttctg aatatcctct
    26821 tattacttta tctccaatgt aatttaattt tgttgtactc gatttatttt cctcttttga
    26881 cttagctttt tctaagtttt gttttgaatc cgttgcagta gggatagcat ctttcttgtc
    26941 tttttcaatt aaatgtattt cctcttcact atctttatta gcattttcca taattccttt
    27001 attagaagcc aatttatcta cattattatt tgtaaaccaa actccaccta ctgctaataa
    27061 acaaacacat acaaacaaag ataaataaaa accatctttt tctaacagct ttttcttcat
    27121 tttgcacctc caaatatatt tctacatttg gattattgac gtgtttttgg cattttatac
    27181 aattattagt atatatcttt tatttttgta tctgtgtagt aatgtgataa tatatcataa
    27241 tatttataac cctcttctgc cattccttct gctccccact ggctcatacc aacaccatga
    27301 ccatatcctt ttaccacgaa atctatgtat ccttctccaa attttatgtc aaagtttgca
    27361 gagttcaact taaaaacagt tctaatgtct tttccagtca attctttatt tcctaatttt
    27421 attttttcta cagttccggc atcacttctt ttagttattg aaacttgttt acttaaatta
    27481 ttcacatcta tcacaatagt gctatatgcc cttctaagac ttttaacaaa atctgtgtta
    27541 cttattttaa gtgttgacgc aaatttaggg gaatacttat cataaggact ttccacagac
    27601 tttaaataag gatattttgc agaaaatact tcttcactat tttctgtttt tcctgaagat
    27661 gtagaaaaat aaagaggaag tatcgcctta tcattataag ttattatttg acctttagtt
    27721 cctctaactg cttcttggat tttggaatat ttattcttaa tccattcttc accatttaat
    27781 tttttaagag tatcataact cttatattct tgacaatgtt tataatcagt acatactact
    27841 gcatttttat gtttactaga tttaccatgt tcctgtttat atactacata tgttctagct
    27901 gctacagatt gagcttttaa agcttctata tcaaactctg aagacatctc accagctagt
    27961 actccacata aatagttctc catgtccatc ttttcggttt tccctgttat atgattatat
    28021 acattgataa ttggtgcttt tttatttaca gtttcatagt tcactgtatt tttttcttct
    28081 ttattacctt tgtcttcaat atcacttttt ttaatagttt tgtttataga tactgggctc
    28141 tctggttttt cagttaattc tacattttta taagaaacta aagttataag tgatggaact
    28201 aacacagagc aagtcacaaa tcctaacaaa acaaccaatg ggttcttcat atactctcct
    28261 ccttagcttt taaaaccttg tatttaagta tatgaacaac cctattaatt tataactttc
    28321 ttctataaac caattattaa tcttctacta tttcgatttg accacctagt gctgttatct
    28381 tcttatctat atcaacataa cctctttgga tatggtatat ttctcctatt tgagtttctc
    28441 cttctgctat aagaccgcat aaaataaggg ctgcccctgc tcttaaatca gtagcgttta
    28501 ctgctgcacc gtgtagctca tctacaccat taacaaccgc acttcttcca tctattttta
    28561 tattagcacc cattctatta aattcagcaa catgcataaa tctgttttca aaaacagttt
    28621 ctataactac accagttcca ttggctacag taagcatagc cataaattgt gcttgaacat
    28681 ctgtaggaaa tcctggatgt ggtaaagttt ttatatctat tggttttaat acttttggac
    28741 ctacaactct tacagaattg tccatttctg ttatttcaca accagcttct cttaatttag
    28801 ctacaactgg ttttaaatgc tccattaaaa cattttcaac agttatatca ccttttgtca
    28861 tagctgctgc aaccatataa gttgctgctt ctattctgtc tggaattaca ttatgttctg
    28921 ctccttttaa ctctttaaca ccttttattt ttatagtatt agtacctgca cctttaacat
    28981 ctgctcccat ttcgttcaag aagtttgcta aatctactat ttctggctct tctgctgcat
    29041 tctctattat tgtagtccct tctgctaaag aagctgccat cattatgttt tcagttgctc
    29101 caacagatgg gaaatctaaa tataatttat ttcctactaa cttttctgtt gctgcttcaa
    29161 caaaaccatg gtccatttct atctttgcac ctaaagcttt aaatcctttt aaatgtaaat
    29221 ctataggtct tgttcctata gcacatcctc ctggcataga aattttcgta ctattaaatc
    29281 tagcaagcaa tggtcccata accaaaaaag aagctctcat ctttcttaca agctcatatg
    29341 gagcttcaca agtttttata ttgcttgcat caacagtaag tgtattttct ttatattcaa
    29401 cttctgctcc tacatgtctt aataaatctg atataacatg aacatctctt aaatttggta
    29461 ctccatttaa tgttgatttt ccgtttgcta ataaagtcgc tgctattatt ggtaaaactg
    29521 cattttttgc accatctatt ttaacactac ctttaagtgg attactcttt tttactatta
    29581 tcttagccat attttttgat ctcctcataa ttaataatct aattttataa ttggtgtggc
    29641 aatatatatg agtgtttttc cgtcatcctc attatattta attcctatat ttaaattaat
    29701 tttttcccct aaatattcta attcataatc atttaatact ttactatatg ctgttataga
    29761 taaaaagttt acgtctttaa cttcatctat ttgcttagca ctcatattat ataatatttt
    29821 ttgtaaaata tcatcatatt tgttgttttg taaccttttt gtgtattctc caacaataca
    29881 agcagacaca tctacttgag atgaatattt atttaacaca ttttctacgt tactataaat
    29941 aacctcttta tgtttatata ctttgttatc caatatgtca actactatgt aactttcttt
    30001 gttattttta tttattcctt ttatagaaat ttctttatcg ctacctttta tagtagcaca
    30061 taattgtatt ttatcgccag accctttttg attccaattt attccttttt tatccaactt
    30121 tagattgtta attatttcta taaaaatatt atgaaattct ttatttgata aattatgatt
    30181 tataacagaa ttaattttaa cattataaaa tttaaaattt gcttctgata tttcaagagc
    30241 tttacttaat ctattgtatc tagtatttat acttgtatct gcataagata ttgtcattcc
    30301 tataatcatt aatataaaaa agcttatagc taattttact tttttcatag taaaaaatcc
    30361 cccttaaata tgtttttctg tataatagaa ttattaacaa atttaagaga gatatacttt
    30421 ctcaatatgt atttttatac atttttgtat ttttcatcta ataatctctt tcaaactctt
    30481 caatctcaat tttctctatc cttctcctgg acttgccaat ttcttatatg tatcaaatac
    30541 atcttgcatt aattcttata tattctcttc tgtaatataa attacttagt ctatacttaa
    30601 atctataatc tataatctat aatctataat ctataatcta taatctataa tctataatct
    30661 ataatctatt catattttac caacaaaata aacagataat acttttttat caatttccat
    30721 agctccaaat gaacatacct aagtacactt cccaatacac aatttacaca ctcttcctaa
    30781 taaatcaaaa acacctttgt tttaaacaac tctattgcac tttcaaaaac tttatttgtg
    30841 ttctcccact tcaattaaat ttgaattatc aagactccag cccatccatc catcactata
    30901 aagggaggta ttttccactc ctatgacatt tgcatatgtt aatacttcag cagcacgcca
    30961 tccacttcca cacataaata taagctgttt attcacatct atattttcct tgtcccacat
    31021 ttctaaaatc tcatatttat ttctcattgt attatcaata ttacgatatt cttctagtga
    31081 aacactatca ctacctgaat gtccatataa agctcctggt atacgccctt ttttatcata
    31141 atatgtatat ccagatactt ttcctatatg ttcatcccaa gtacggttat ctactagtat
    31201 aaacttattt ttttctttta gtttttgtct taattcagat gtagtaacaa ttaattgtga
    31261 gttaactggt atatctgctc caaaattagt acatgaatgt tttggattac taataaattc
    31321 taattcatat cctgctgata accatgagtt tgttccacca ttcaaaactc ttacatcttt
    31381 tactcctata tatctcaata taacagctag tctataagat gccataggtg tactacttga
    31441 aactattaca gtatcatcct ttgttaatcc atagtctaaa gcaaacttag ttaagttatc
    31501 atcattatca agcatccatg ttggaggagg ttctataata tcagtattta catgaacact
    31561 tgtaggtata tgcccttttg tatacgattc ttctctccaa ctcgcttcaa tcatttttat
    31621 attctttgaa tcttcaaatg tctcaggaat ttttccatct aatatatctt ttattataaa
    31681 agcaggaact atcatttgat aatttttata tctttccatt ggaagatttt cgtcgtctgc
    31741 ccattgttta atatcatatt tatatagata tttatatcct tttttagata aataatctgc
    31801 tacaaccaaa gcatcttttc catttgcatc atacaatact atatttttat ctaaatcaat
    31861 tcttttagtt ttcaatgctt gttctaacac ttcatctttt ctatctgagt atacacttaa
    31921 ccaattagca gaaaaatcta ctgctccttt tatatgtcca cctcttttta ctccatctaa
    31981 cttccagcca ttataagcat catttaagcg agtatcaact ataacccaag cgttatcttg
    32041 cactttttct tgtaaaactt cagtagatat tgccttaaga ttaacaaaat aatcttcttt
    32101 cttttctttt ttaaaatcat ttgtcataat ttatactccc aatttaaaaa aatttttatt
    32161 tgaaattatt ttactaaaat aaagtaattt tcttatactc tattagcttc attatattct
    32221 tttataaata gttccttata ttcttatttt aaactactca ctttatctta tcacaaggtt
    32281 actaatacct tgtattttaa tttttctcat aagctattat ttttttatct gatattgact
    32341 atatgtttat taagtactca ctatatttta tatgaactat atttatcgtt ccctatacct
    32401 ggtatggtct atatatttat cattttacag atgatttaat atcaatcttt ttataaaatt
    32461 tattatagca aaaacaaaac aaatttaata tttatctaaa aaactcatcc tataaaatac
    32521 aaataacagg atgagttctt ttcaataaat attatctatt taatttttaa tctgttttta
    32581 ttcaactgtt actgatttag ctagatttct tggcttatct acatcgcatc ctcttagtac
    32641 agagacatga taagcaagta attgcattgg tacaactgct aatattggtg ataaaatatc
    32701 atctgaattt ggtatataga ttattttatc agcagctttc tcaacatcct tattatggct
    32761 ttgagctatt gctactacat aagctcctct tgctcttact tcttccatat tagaaaccat
    32821 tttttcaaat aacttttctt gagtagctat ggcaattaca ggag
//
LOCUS       DAGSHT010000021        32655 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGSHT010000021 DAGSHT010000000
VERSION     DAGSHT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.022"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..353
                     /locus_tag="MJB72_000949"
     CDS             <1..353
                     /locus_tag="MJB72_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBY8262074.1"
                     /translation="IIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEIDLDM
                     VNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNESLIL
                     NNLKNLEKQYNSSY"
     gene            366..878
                     /locus_tag="MJB72_000950"
     CDS             366..878
                     /locus_tag="MJB72_000950"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBY8262075.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            952..3159
                     /locus_tag="MJB72_000951"
     CDS             952..3159
                     /locus_tag="MJB72_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBY8262076.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3184..3633
                     /locus_tag="MJB72_000952"
     CDS             3184..3633
                     /locus_tag="MJB72_000952"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBY8262077.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3648..4262
                     /locus_tag="MJB72_000953"
     CDS             3648..4262
                     /locus_tag="MJB72_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8262078.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4353..5909
                     /locus_tag="MJB72_000954"
     CDS             4353..5909
                     /locus_tag="MJB72_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBY8262079.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5899..7161
                     /locus_tag="MJB72_000955"
     CDS             5899..7161
                     /locus_tag="MJB72_000955"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBY8262080.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7235..9022
                     /gene="aspS"
                     /locus_tag="MJB72_000956"
     CDS             7235..9022
                     /gene="aspS"
                     /locus_tag="MJB72_000956"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBY8262081.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9481..10713
                     /locus_tag="MJB72_000957"
     CDS             9481..10713
                     /locus_tag="MJB72_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBY8262082.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            10727..11533
                     /locus_tag="MJB72_000958"
     CDS             10727..11533
                     /locus_tag="MJB72_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBY8262083.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11645..12514)
                     /locus_tag="MJB72_000959"
     CDS             complement(11645..12514)
                     /locus_tag="MJB72_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8262084.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            12886..14313
                     /locus_tag="MJB72_000960"
     CDS             12886..14313
                     /locus_tag="MJB72_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBY8262085.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(14390..14659)
                     /locus_tag="MJB72_000961"
     CDS             complement(14390..14659)
                     /locus_tag="MJB72_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262086.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(14662..16104)
                     /gene="nhaC"
                     /locus_tag="MJB72_000962"
     CDS             complement(14662..16104)
                     /gene="nhaC"
                     /locus_tag="MJB72_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBY8262087.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(16129..17295)
                     /locus_tag="MJB72_000963"
     CDS             complement(16129..17295)
                     /locus_tag="MJB72_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY8262088.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(17520..19640)
                     /locus_tag="MJB72_000964"
     CDS             complement(17520..19640)
                     /locus_tag="MJB72_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8262089.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            19873..20274
                     /locus_tag="MJB72_000965"
     CDS             19873..20274
                     /locus_tag="MJB72_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262090.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            20317..20610
                     /locus_tag="MJB72_000966"
     CDS             20317..20610
                     /locus_tag="MJB72_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBY8262091.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            20625..21065
                     /locus_tag="MJB72_000967"
     CDS             20625..21065
                     /locus_tag="MJB72_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBY8262092.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(21157..21630)
                     /locus_tag="MJB72_000968"
     CDS             complement(21157..21630)
                     /locus_tag="MJB72_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY8262093.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(21630..22418)
                     /locus_tag="MJB72_000969"
     CDS             complement(21630..22418)
                     /locus_tag="MJB72_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY8262094.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIAFSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            22746..23990
                     /locus_tag="MJB72_000970"
     CDS             22746..23990
                     /locus_tag="MJB72_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBY8262095.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            24313..25410
                     /locus_tag="MJB72_000971"
     CDS             24313..25410
                     /locus_tag="MJB72_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBY8262096.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            25401..26099
                     /locus_tag="MJB72_000972"
     CDS             25401..26099
                     /locus_tag="MJB72_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY8262097.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            26102..27166
                     /locus_tag="MJB72_000973"
     CDS             26102..27166
                     /locus_tag="MJB72_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY8262098.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            27349..28053
                     /locus_tag="MJB72_000974"
     CDS             27349..28053
                     /locus_tag="MJB72_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8262099.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            28046..29368
                     /locus_tag="MJB72_000975"
     CDS             28046..29368
                     /locus_tag="MJB72_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8262100.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            29555..30928
                     /locus_tag="MJB72_000976"
     CDS             29555..30928
                     /locus_tag="MJB72_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262101.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(31300..32238)
                     /gene="pdaA"
                     /locus_tag="MJB72_000977"
     CDS             complement(31300..32238)
                     /gene="pdaA"
                     /locus_tag="MJB72_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBY8262102.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        12367 a         3903 c         5472 g        10913 t
ORIGIN      
        1 aaattataaa atattataat aaaacagacc tattatattt aaagaatgta gttagtaata
       61 tagttccatc aagagatgaa tttataacaa tttataaaca agcactcata aaaaaagaaa
      121 ttgatttgga tatggtaaat ataagggaaa cttttaatgt aattccatta aagtttttta
      181 cgatattaaa tgtatttaga gaattaaatt tacttgactt taatttaaat tatgaaaaaa
      241 attctgtact cataagaata ttgccaaaac cacagaaaaa attagactta aatgagagtt
      301 taatactcaa taatctaaaa aatttagaaa aacaatacaa tagcagttat taggaggcgt
      361 ttcccatgga tttaaaaaac tttataagga atattgatga tttcccaaag ccaggcatag
      421 attttaaaga tgtgacaact ctatttaaag atggagatgc atttaaatat gcagtggatt
      481 ctatagttga agaattgaaa gataaagatg tagatttagt aataggacca gaagcaagag
      541 gatttttgat gggtacacct gtagcatatg ctttaggagt aggatttgtt ccaattagaa
      601 aacctggaaa gttaccaggt gaagttgaga gttatgagta tggattagag tatggtacag
      661 atactttaga gatacataag gatgcaatta aaaagggtca aaaagtagca atagtagatg
      721 atttattggc tactggtgga actatggaag ccgctgccaa gcttgtagaa aaattaggtg
      781 gagaagtagt ttctatgcaa tttttgattg agctaaaatt cttaaatggg agagaaaagt
      841 tgtctaacta tgatgttaat tctcttataa aatactagta cttttataat agttggtata
      901 acaccaacta ttttcatata tattaaaatt ttatacaaaa taggtggtta tatgcatgat
      961 aaagaattac aagaaataat tgaaaaaata aaagtttatg cacctaatgc aaattttgaa
     1021 atggttgaaa aagcctataa tcttgcaaaa aaagcgcatg aaggacaata tagaaaatct
     1081 ggggaaccat actttattca tccattagca gttgcaaata ttttaataga gatggaatta
     1141 gatattgaaa ctataacagc aggtttactt catgatgttg tagaagatac agaatatacc
     1201 tatgaagaca taaaaaaaga atttggcaaa gaagtagcgg atttggttga tggagtaact
     1261 aaacttggtc aaataaaata tcgttcgaaa gaagaaactc aatctgaaaa tttaagaaaa
     1321 atgtttttgg caatggcgaa ggatataagg gttatactta taaaacttgc tgatagattg
     1381 cacaacatga gaacattaaa atacatgcct ccagaaaaag caaagtataa agcaaaagag
     1441 acacttgaaa tttatggtgg tatagctcat agattaggaa tttccaagat aaagtgggaa
     1501 ctagaagaca gagcattaag atttatggac ccagaagggt attatgattt agttagtaga
     1561 gtatcaatga agagaagtca aagagaggat tatattcaag gaattgttga acttcttaaa
     1621 gataagttta aagatttaag tataccatgt gatgtatatg gtagaccaaa gcatttttac
     1681 agtatatata gaaagatgca aaaaaaacat aagacttttg aagagatttt tgatttaaca
     1741 gcagtaagga tattggttga taatgtaaag gattgctatg cagtacttgg tatagttcat
     1801 acattatgga gacctattcc aggaagattt aaagattata tagcaatgcc aaaacccaat
     1861 atgtatcaat cattacacac cactgttgta ggtcctgatg gagaaccttt agagatacaa
     1921 ataagaactc atgagatgca taatatagct gaaaatggta tagcagccca ttggaaatat
     1981 aaagaaggta tctctagcaa agaatcaaag gttgaagaaa aacttcaatg gttgagacag
     2041 atgatggagt gggaaaaaga tttaaaagac ccacaagagt ttatggatgc acttaaagaa
     2101 gatgtattta atagtcaagt ttatgtattt acgccaaaag gagacgtaat tgaattacca
     2161 gcagaatcaa cacctataga ttttgcctat agggttcata caaatgtagg caataaatgt
     2221 gtaggagcta aaattgatgg aagaatagtt cctattgatt ataagcttca aaatggtaat
     2281 attgtcgagg tacttacatc atctaattct aatggaccaa gtagggattg gcttaatata
     2341 gtgaagaccc caaatgcaaa aagtagaatt agacaatttt tcaagaagga aagaagagaa
     2401 gaaaatgttg accgtggaaa tacaatatta gaaagagaat ttaagaagca tgatatacca
     2461 caaaaagacc ctcttgttga aaaatatatg gcattagttg ctaagaaatt taatcagcca
     2521 agtgttgagg acttaattgc cactgtaggt tatggtggta taatgtcatc tcaagtggta
     2581 tctaaggtaa aagacctata tttaaaagag gttaaaaaag ctaaaaagga acaacagata
     2641 caagaagaag atttaaataa acacaatata agtgaaacag aatacagtaa aaagagaaag
     2701 aaaaacgcat cacaaggaat tatagtaaaa gggcttgaca atatcttagt aagatttgct
     2761 aagtgttgta atcctcttcc aggagatgaa attgtaggat atattactaa gggtagggga
     2821 gtagccatac acagagctga ttgcccaaat gcaaacaata atggtgattt cttccaaaat
     2881 agattggtgg aggtttcatg gagttcagca ggaaaaggta agtttgaagc ggaaattcaa
     2941 ataaaagcta ttgatagaag aggaattgtt aatgaaataa ctcatattgt tgcaatggat
     3001 aaacttagtt taaacggaat aaatgcaaga aaaggtaagg atagtattgt aagtataaac
     3061 ttacttgtag aggttaatga cacagaggaa ttaaaaattc taatgaaaaa attaaaagca
     3121 attcctggtg tagaaagtat ttatagaatg acaaattaga gttaaaataa tagggaggaa
     3181 actatgagag cagtagtgca aagagtatct tcatctaaag ttacagtaga tgaaaacact
     3241 ataggtcaaa taaataaggg gttactagta ttgttaggag tgactcatga tgataaatca
     3301 agtgatgtag attatatgat tgataaaata ttgaatctga gaatttttga agatgaaaat
     3361 gacaagatga atttatctct aatggatata ggtggagagc ttttagtagt atctcaattt
     3421 actttatatg gagattgcag aaaaggaaga agacctggat ttagtaatgc agctaggcca
     3481 gaacttgcaa ataaccttta tgaagaattt gtaaaaaaag caaaagataa aggtgtgaca
     3541 gttggtacag gtcaatttgc agctcatatg atggtagagt taactaatga tggtccagta
     3601 acaatactat tagactcaag taaatctttt taagggggaa aactttaatg attataaaaa
     3661 aatttgtaga tagttatttt ggagtaaata cttatgtatt aggtgatgaa aagactaaaa
     3721 aatgtgcagt tatagaccca ggtggaagtt tagtagagac attaagtttt gtaaaagaaa
     3781 atgaattaaa tattgaatat attatcctta ctcatgggca tggagaccat ataggatatg
     3841 ttaaggatat taaagaaaaa actggtgcta aagtagttgc acatgttgat gaaaaagaat
     3901 tattaaatga caaaaataaa aatctaagtt atacaatgag atgtggagcg caagagtttg
     3961 atgcagatat ctatgtaaat gataaagaca agcttgaact tggtgaatta aaaatgactt
     4021 tcttgcatac tccaggacat actcaaggat gtatgtgtat aagagttgaa gatgaaatgt
     4081 tcacaggaga tactttgttt gctggaagta ttggtagaac tgatttatat agtggagact
     4141 ttaaccaaat ggaaaaatca cttaagaaat tatgtaaata tgaagatgct gttagagtat
     4201 atcctggaca tggaccaacc agtactctag gggttgaaaa gaagacaaat ccttacatgt
     4261 aattttatat tagtatttaa aaaagtataa aatatttaat aactatatta gtgagggggt
     4321 tcttctactg ttaaatagaa ggagttaagt tgatgttgta tgttttttta aaaggacatg
     4381 attataagta tgaagtagca gaacttataa aattgtttac tagtgaattt aagtttaaag
     4441 actctaatgc taacaataat atagagtcaa agcatttaat aaatagattg atttatgaga
     4501 atggtgtatt attctcaagt actgaatact atgaaaatgg aaacttaaag tacgagtcaa
     4561 tacagaatat agaaaatatg aatcttgaat ttttagatgg atttgatagc agcaaaacga
     4621 ttgaagaatt tcttaatagc ctagatagtg aaagtaagag gaactttaaa aagctgtgta
     4681 aagaaaatat caaaaaaagc atgtttttag tgcttaaaca agtatttaat tcttatgtac
     4741 catggggaat tttgactggt attagaccag tcaaaattgt ccacaattta atggataaaa
     4801 agctagatga taatagcata agaaccattc taaaagataa ttattttata atagatgaga
     4861 aaatagattt agctcttgaa atagctaaaa gagagcgatt atttatgtat cctatagata
     4921 aaaataaaat ttctctttat gtaagtattc cattttgtcc aactagatgt gtatattgtt
     4981 catttccttc aaattcatta aaacaatttg gacatttaaa aagagaatat gtttataaat
     5041 tgattgaaga aattaagggg tttgccaaag ttattaagga tactaagaaa gaagttgaaa
     5101 ctctatatat tggtggtgga actccaacta cacttgatga ggaagaattg gatttgctta
     5161 taaattcatt gttcaatgaa ttagatttaa gtaaaattaa agaatttact gtagaagcag
     5221 gtagacctga cactattaca gaacaaaaac taagagtgtt aaaaaaacac aatgttagta
     5281 gaattagtat aaatcctcaa actatgaatg atgatacctt ggtaaagatt ggaagagagc
     5341 atagagtatc tgatttagta gattgcttta atatggctcg tagaatggga tttgataata
     5401 taaatatgga tataatttta ggtcttatag atgaagactt aaatatggta aagaacacat
     5461 tggaggagat aaaaaaactt tctcctgaaa gccttacagt tcatacatta gccataaaga
     5521 gagcatctaa cttaaatata aatatggata ggtataaaga acatctaaca caatatgaag
     5581 aaatggtaaa aatgatagat ttatcaatga aatatgcaaa agatatggga ttaaatccat
     5641 attatatgta cagacaaaaa catatgttgg gaaatttaga aaatataggt tatgctaaag
     5701 aaggatatga gtgcatttat aatatacaga ttatggaaga aaaacaaagt aactatgcat
     5761 taggtgcagg tgctatatcg aaatttgtct atgtagatga agatagaatt gaaagagtag
     5821 aaaatgtaaa aaatgttgaa caatacattg aaagagtaga tgaaatgata aaaaggaaga
     5881 aagaagaggt atataaaaat gttgactaaa gctcctagag gaactaaaga cataactcca
     5941 aaagaggctt acaaatggcg ttatgtagaa aataaattta gagaaatatg tgctttatat
     6001 ggatatgaag aaatggtaac acctatattt gaacatacag aacttttcaa gagaagtgtt
     6061 ggagatacta cagatatagt tcaaaaagaa atgtattctt ttaaagataa aggtgataga
     6121 gaaattaccc taaagccaga aggtacagca ggtgtagtaa gagcatttat agaaaataaa
     6181 ttatatgcag atactcaacc aacaaaatta ttttatgtga caccttgttt tagatatgaa
     6241 agaccacaag ctggtagaca aagacaattt caccaatttg gaatagaagc acttggaagt
     6301 gatactcctt caatggatgc tgaaataatc gcactagctg ttcaattttt taatgaagtt
     6361 ggattaaatg atttggtagt gagtataaat tcagttggct gtccagtttg tagaaaagag
     6421 tacaatgctt tattaaaaga gtatcttgac tcaaaagctg atatattgtg tgatacttgc
     6481 aatgagagaa gagaaaaaaa tccaatgagg gttatagact gtaaaaatcc tacatgtaaa
     6541 gaaaatatca aagatatacc atttattgca gaccatttgt gtgatgattg taaatctcac
     6601 tttgataaac ttcaagaata cttaaaggaa atgaatataa actttgtaat tgataaaaca
     6661 atagttagag gcttagatta ctatagaaaa actgcttttg aaattatatc taatgatata
     6721 ggagctcaaa gtactgtatg tggtggagga agatatgatg gtcttgttga acaattagga
     6781 ggaccaaaag gtataagtgg aattgggttt ggactaggaa ttgaaagact tttacttaca
     6841 cttgaaggta atggaattga aatagaaaac cctcaatcta cggatatatt tatagtaact
     6901 ataggtgaag aagcaaatac aagaagcttt aaattgttaa aagacttgag acaaaatcat
     6961 ataagtgcag ataaagacca tatagagaga agtgttaaag cacaatttaa atattcagat
     7021 aaaataaatt caaaatttac aatagtaatt ggagatgatg aacttaaaaa tgacactgcg
     7081 acattaaaaa atatgaaaac atcagagcaa actactgtaa aattaagtac attagttgaa
     7141 gaattaaagc aaaagctgta aaataagaaa gtatgatgtt ttgtgtatta gataaatata
     7201 tagcattaaa attaaataaa atggaggtag tatcttggaa actttaaagg gtttaaagag
     7261 aactcactac tgtggagagt tgagagaaaa aaacataaat gaagaagtcg tacttatggg
     7321 atgggtacag aaaaaaagaa atttaggtgg tcttgtattt gttgatttaa gagatacaag
     7381 tggattatgt caaatagttt ttgatacaga tgtagataaa gaagcttttg aaaaagctga
     7441 aaaattaggg gctgaatttg taatagctgt aaaaggaaaa gtatgcgaaa gacaatctaa
     7501 aaaccctaat atgccaactg gtgatataga aatatttgca actgaactta gacttttaaa
     7561 caagtcagaa actccgccta tttatataaa agatgatgat gatgtttctg aagcactaag
     7621 attaaaatat agatacttag atttaagaaa accatctatg caaagaaatt taaaattaag
     7681 acataaagta atgaatatta caagaaatta cttatcaaac aatagatttt gcgaaataga
     7741 aacaccattt ttaatagctc caacaccaga aggagcaaga gattatctag taccaagtag
     7801 agttaatcca ggaaaatttt acgcattacc acaatcacca caattatata aacaattatt
     7861 gatggtaagt ggtatggata gatatttcca aatagttaaa tgctttagag atgaagactt
     7921 aagagcagat agacaaccag agtttacaca aatagactgc gaaatgtctt ttgttgagca
     7981 agaagatgtt atgagtatga ttgaaggttt gcttgaagcg atatttaaag aagttttaga
     8041 tgtagaattg gcacttccac ttcctaagat gacttatgca gaagctatgt ctaaatatgg
     8101 ttcagataaa ccagatacta gatttgggta tgagttaaca gatatatctg atgttgtttg
     8161 taactgtgga tttaaagtgt ttgctgatgc tacacaacca ggaaaaagtg ttagaggtat
     8221 aaacgtaaaa ggcaaagctg acgactttac tagaaaacaa atatcatcac tagaagaaca
     8281 tgctaagaca tatagagcaa aaggtcttgc ttggatgaaa gtaggacaag aaggagtaac
     8341 ttctccaata gcaaaattct tcaatgaaga agaaatgaac gctatactta caagaatgaa
     8401 tgctgaagta ggagatttac ttttatttgt agctgataaa aactcaatag tatttgatgc
     8461 tttaggacaa gttagacttg aagtagcaaa taggcttaat ctattggata aaaatgtata
     8521 taacttacta tgggtaactg agttcccagt ttttgaagaa gatgaagaaa caggcacatt
     8581 ctcagcaatg catcacccat ttacatcacc tatggatgaa gatttagaca agttagaaga
     8641 aggtgataag tcatctttaa gagcgaaagc ttatgatatt gtattgaatg gttatgaaat
     8701 aggtggagga agtgttagaa tatcaaattc tgatgttcaa tcaagaatgt ttaaagcact
     8761 tggttttaca gaggaaagag ctaatgaaaa gtttggttat ttattagaag catttaaata
     8821 tggtactcct cctcatgctg gtctagcttt tggtcttgat agacttgtta tgttacttgc
     8881 aggaactgat aatataagag aagttattgc attccctaaa aatcaaaatg ctgtttgccc
     8941 tatgacaaat gctcctacat tggcggaaga tgagcaatta gaagaattaa gtataaaagt
     9001 tgatataaaa gataatgaat aatatgtagt tgcaagaaac taaaatgtaa aaagtctgga
     9061 tataaaatat atttatattc agactttttt tattagaatt attttatatt ttttctctat
     9121 atattttcat aaattatact tatattctta accaatataa ttaaaatact atgtttaaaa
     9181 attttacata aacttggttg ttttagaaac tacttctgta atgagtacac gattttaatt
     9241 aaagaatata taacaaattt gaaataagtc taatatatga cttaatagat taaattaaaa
     9301 taataaattt ggactattta tctatttata acttaaatta tatcaaattg ataacaaact
     9361 agatattata cttttttgta gaatagaatt acaattatat aaagaaaaat tctattaata
     9421 gtgaaaaaaa agtagaaaaa agtagaaatt attcttttat aaagaaaggg agggataata
     9481 ttgcaagaga gagaagaatt agttttaaat ccagttccaa aagaggaaag agttggatgg
     9541 ttagctccat tgtttaatat gcttggttct aacatagcta tatctgaact aatggttggg
     9601 ggaactttaa ttttaggaat gactctttct aatatgatta ttacatcaat tatagggaac
     9661 ttaatattag ttgcaattat tatgatacaa ggttatatag gttacaaaga agggttaaat
     9721 acgtatgtat tagctaaggg ggcatttgga gagataggag gaaagtatct tatatctcta
     9781 ttacttggta taactagttt tggatggttt ggtgtacaag caggggttgc aggactatca
     9841 gtacaaaaga tattcccaag tgtaaaccta acattagtta cagttatact tggattattt
     9901 atggttgttt ttgcactgta tggttttaaa gctatggcta aatttaatta cttagttata
     9961 ccaccactta tgatattaat ggtgtgggga gtttttaaag ctttttctac ttatggagtt
    10021 gaagctattt ataattatac tcctcaaaca actatgagta tggttgaagg tcttaatatt
    10081 gtagtaggtc ttgttatagt tggtgcaatt atatcacctg accagttaag atatacgaga
    10141 cgagtaaaag acatttggat tatcagtttt ttaggtctag ggattatttc attattccaa
    10201 caagttgcag ctggtgtaat gtctatggga gcacctactt gggatataac agaagtattg
    10261 gctaatttag gttttggatg ggtagcgttt gttattttaa tacttgcatc ttggtctaca
    10321 aacgtatcaa atgcttattc tggtggatta gcattaaaaa ctatttttcc aaatgtaaaa
    10381 agaaatgttt tgactttagt tgcaggtcta ataggtacaa taatagcagc tactggtata
    10441 atatttaaat ttcagtcatt tttatctttc ttgggaatcg cagttcctgc aatagcagga
    10501 attatgtggt gtgagtatta ttttatacaa ggaagaactt ataagcatag agaaggaata
    10561 aattggattg cagtaataag ttggttaata ggttttgcag cgtcgtatta tagttcaaaa
    10621 ataaatttct taatacctcc gataaatgga attgtagtga gtatggttat ttattatata
    10681 ttaatgaatt gttttggaat caaagataaa taaagggggc aattacatgg gtcgtaaggt
    10741 gaaaataggt ataattcaac aacacagtgt acttggaaat gttaaaaaaa atatagaaaa
    10801 agcagtagag atgatagatg acttaggaaa acaaggggca gatataattt gtttaccaga
    10861 actatttgct acaggatata atttagagtc tttaggtgga gttaaaacat tagaattaat
    10921 aagagaacat aataagtaca tagaggaatc aatgtcagaa gctgctaaaa gaaataatgt
    10981 atacctgata tctccatatg gaaccttaga aaaagggtct acacatgttt ataattcagc
    11041 agttatattt gatagaaaag gtaagattat gggagaatac tgtaaaaatc atctgtggtc
    11101 attagaagca gtatacttta aaggagggga aaaagtagag gtatatgatg ctgattttgg
    11161 tagatttggt gtaatgatat gttatgatgc aggttttcca gaagtatcca gagaattgac
    11221 attaaaaggt tctgaaataa tatttatacc atctgcatgg agaatacaag atgaagatat
    11281 gtgggattta aatgtatctc aaagagcttt agagaacact gtatatacag taggtgtaaa
    11341 tttagtaagt aatgactcta atttaatact atttggaaag agtaaaatat gcaatcctag
    11401 aggtactgta ataactcaat tagatacata cagggaaaaa tatgtattat cagagattga
    11461 tttagatgaa ataaaaaagt atagggaaga tatcccctat ttgaaagaca gaaaaagtag
    11521 ctataacata taattctata ctataataca agttcaatat aaaatgataa aaagaggatg
    11581 tatcaatatt aagattaaat ttattatact ttgatatatc ctctttaata ttatcaactt
    11641 tgatttaaat atttttaaaa tagttgataa gaaagttttt gaacaacaac acagctttag
    11701 attcatagtt ttcgctaaac catgaaagat ttaaatatct tttaaattca atatctttga
    11761 tatgtaacaa cttaatctca gggtcttgag ataatcccca tgattttcca catataaatc
    11821 caactccttg aagtgatttt ataagagcat aaattgtata tggtgaatca gactcaaatg
    11881 ctattttggg tttgaaatta gctgattcgc acaggatatc aattacctct ctataattct
    11941 cacccttcgt tataactata aagttttcat ctgaaacttc gcttagatat acttcatcct
    12001 ttaatgataa aggatggttt attgaaactc ctaatagtat ttcttcgcat gttaaagtta
    12061 tgctattttc tgagtttagc tttgtaaatg aagaagatat gtacaaatca aaatcacttt
    12121 ttttataaga tgatggcaaa gtatgtgaca cattaaaagt aatattggga tatagttttc
    12181 tgaaattact caaaagtgat ggtaacacat tagccgctgc aggagataat atctttactt
    12241 ctcctgaatt ttccaaagaa gtgtcttgta actccttttt tgaactttct actaaatcta
    12301 aaatgctatc tactcttttt agaaacaatc ttccatactc atttaaaaca atatatcttc
    12361 cttttctgtc aaataactga actccaagtt ctttttctaa ggaagatata gttttgctta
    12421 aagctggctg tgctatattt aacttgtgtg atgcttttgt tatgtgctcc attctagcaa
    12481 cagtttgaaa atacttaaga tgcaataaat ccatagtata aaatacacct cttttaatat
    12541 gacttgagtt atatcaattt tataatcaac tccacattat acattataat ataaagttta
    12601 tataataaac tacaatatat taaaaagtta tttaaattgt tatttatatt ttgtacataa
    12661 tctaattaaa aaaagtaact ttaaatggtt aactaaggaa atattgttac aaactttagt
    12721 tatataaaaa attcgtttga tgagcaaagt attaatgtca agtaagaata aaagcttatt
    12781 agataagaaa aataatattt tataataatg atgttactta aattattatg ttatactatt
    12841 ttaaatagga aacatttaga tactataatt ttagggggag tcaagatgaa attaactaaa
    12901 tcaatatcat caatgctgtg cttatctcta gttttaagct tatcaccagt agcatatgca
    12961 aatgaaataa gtagaacaac agttgagaat actttagtag gtcaggatag atatcaaact
    13021 gctgttgaag taagtaaaaa aggatggagt agtgcaaatg aggctgtagt tgtaaatagt
    13081 gaatctattg tagatgcatt agctgtaaca ccatttgcta aattaaaaaa tgctcctata
    13141 ttattgacag aaaaaaataa tctaaattca caaactgaga aagaattaaa aagattagga
    13201 gttaaaaaag tttatataat tggtctatct agttctgtat caaatgatgt gcaaaaacag
    13261 ttagaatcta ataatttaac tactgataga gttggtggta gtgatagata ccaaacagct
    13321 ttgagtatag ctaaaaaaat agaaggatta aaagatatat cagaaatagc tgtagtaaat
    13381 gggtacacag gtcttgcaga tgcagttagt atagcttctg tagctgctac taatggaatg
    13441 gttatacttc cagtatctga tgcatctggt atatctagct ttaaagattt tatagaatct
    13501 aagaatataa ataaatcata cataataggc tctactaatg ctgtatcaga taaaataaaa
    13561 caaagtttac ctaattcaga aagaataggt ggagcagata gaaatgaaac taatggtaaa
    13621 gtaatagaaa agttttatac atctaataaa ttaaacaatg tatttgtggc taaggatgga
    13681 atgaaaaatc aaaatcaact tatagacgct ttagcagtag gagtactagc atcaaaagaa
    13741 aattctcctg tactaatcgt aggagaacaa ttaagtgcaa cacaaaagag tgtattttcg
    13801 aataaaaatg ctacctctat aactcaagtt ggtagtggag gaaatgaaaa tgcatttaaa
    13861 gaattaaaag atttacaggc aaatagtaac aataagagag taatgtatgt aactaataca
    13921 gataaggtaa atataagaaa tgatgctaca atagaagctt ctgttatagg atatctaaat
    13981 aatggtgatg aagtagaagt tttagatgtg ttaaaaactg gatgggtaga aataaaatac
    14041 aatgaaggta ttggatatgt tagtgggtct tatttgacaa ataataagcc tgataatagt
    14101 aatgaaaata taaaaataaa gtatgtaaaa gaaaaagatg gtctaaatgt taggaaagga
    14161 ccaagtacag aagatgaaaa aataggtcac ctttcatatg gaagcaaagt agaaacaata
    14221 gaaatgtttg ctactggatg ggtaaaaata aagtataatg gtggatatgg ttatgtaagt
    14281 aatgattact tatctgatac accagttatt taattataag taacaataaa aaaactgctt
    14341 taatttgtaa agcagttttt ttataaaaat ttaagtctaa taactatttc tattttgata
    14401 ccttaatcct gggatttatt attatggata ttatcaacat acaaacactc caaatccagt
    14461 gtctaattcc aaacttagat attaataaat tcagtcctgc tatggtaaat aagcagtatt
    14521 taattacaat taaaagtgta tttatagaaa gccccttcat agaaagtatc ctaaatccaa
    14581 taatagacat tctaagtcct ataaatgcca ataatatacc tgcaatatcc tgaattaagt
    14641 aatagtattc aaatttcata ttcaatcaac tcttctctga gcagtatcaa atatttctaa
    14701 ctcttcaatt tcatcatctg tgtatgtaag catagtaaaa ccagttacac cataaataaa
    14761 tgaaattata ggtgtaatcc aacttaagaa acaatatgga ataaattcta gtgggcttac
    14821 accaagagtc tgagcacaga aaacagcatt tgaattccat ggtattaatg gaccaccaag
    14881 tgtagctgta tcttctataa ttctagatag attttgtggt tttaatttaa attctttgta
    14941 tattggagac attagagttc cgacaaatac agaagtgaac atcattgatg aactaaatgc
    15001 atttgcaaag taacttacta taaaagtaga cccaaccaat ttagttcttg attttcctat
    15061 tttagttata agaggttgaa gcagtacttc taagaatcca gttttttgga gaataccagc
    15121 aataacgaaa actacaaata ctagtaaaac ggtttcagcc atactcatta ttccaccacg
    15181 atttaatagt ttgtcggcat agacaaatcc agtatctata gtaaatcctg atagcatgta
    15241 atttaaaaca tcacctattt tttctccttc ttgaaatact gctataataa gtcccatgat
    15301 agctgaactt agtatagata taatgggtgg cttttgtagc aataaaagta ataataaaaa
    15361 tattattgga atcattgcta ctaaaccaat atgaaagttt gaatttaata catccttgat
    15421 ttgatttatt tgaacatagt ctatagtatt atttgaatac ttaaatccaa ttactgtata
    15481 cagaactata catattacgt atgcaggtcc tgtggtataa aacatatgct tcatatgtgt
    15541 aattacatct gtcttacaaa ctgctgcagc taagtttgta gaatctgata gaggtgacat
    15601 cttatctcca aagaaagcac cagaaatgac tgcaccagct gttaaaccaa gaggaatacc
    15661 catactagta ccaatactca tcattgctat accagaagtt ccaagagtcc cccaagatgt
    15721 accagtagca acagaagtta atgaacatac gattaatgtt gtaagcagaa aatatttagg
    15781 ggttattatt gttagtccac tatatactac tgttggtatt gtaccagcag ctatccaagc
    15841 tgctattagt acaccaacag aaagtataat taagtttgat tgaaaggcat ctttaccaac
    15901 ttcaaatcca aaagcttcta tttcatcatt agtataacca agtttcatag ctgcaggaac
    15961 tactataagc catgaaagta aaaacattgt agttatagaa gcattaaata caactatacc
    16021 aactgatgtt agagctatta ttgcaagcat tactaacaag gaatatctaa atgaaggtat
    16081 ttttctttct gtattcattt tcatagtgca aattctcctt aaatattttt agttaattgc
    16141 attttttatt cttataagag cttcttcaag catacttctt ggacaagcta agtttattct
    16201 ttgataccca cttcctccaa tgccaaagct gtttccttga tttagagcaa ctttaccttt
    16261 ttgtacaagt atactttcta gttcctcatc acttaatcca agtgcactaa agtctaccca
    16321 taataaataa gtaccttctg gttttctaac tttaagtttt ggcatatttt catttatata
    16381 ttttatagca aaatctatat tagattctaa atattctaaa aatgattcta gccaagattc
    16441 accattatta tatgaagctt cagttgcaac taagctaaag caattatttc tctttatatc
    16501 aattctggta aaggcatcat ctaacaattt atagtctttt tcatcaggta gtattacata
    16561 tgatgattga agaccagcta tattaaaagt tttagttgga gccatacaag taattgtatt
    16621 tttttcaaat tctttagaaa tagatgccat aggaatatgc ttatgttttt ttaatataat
    16681 gtcactatgt atttcatcag atatgatttt tacattatgc ttaagacaaa tatctcctag
    16741 tttttttaat tcatcttttg tccatactct accaactgga ttatgaggat tacaaagtat
    16801 gaataatttt acatctttaa tcttgttttc aatgtcttca taatccatta tgtagtttcc
    16861 attttctagt ttttgtaatg ggcttataat tagttctcta ttgttatttt ttacaacact
    16921 attaaaaggg ctgtaaactg gttcttgtat cattatttta tcatttgctt ttgtgagttc
    16981 atttattaaa agacttatag ctgggataac acctggacta taaattaacc actcactttt
    17041 tattttccag ttatgtctcc tatctagcca gtttactata gattcattgt aagaatctgg
    17101 tcttgttgtg tagccataaa tctcttgttc tagtctattt tttaaggaat caattataca
    17161 aggtgcagcc ttaaaatcca tgtcagccac ccacatagga agtaaatcat tagttccata
    17221 ctttttttcc atttctgacc atttagatga aaaattattg cttctatcaa caatctcatt
    17281 aaaattataa ttcattgtaa atacctccag taattttaat tttatgttaa taatattaag
    17341 caaacaacgt gccaatattg ataataaaaa atatttattc acaagttaat atttaatatt
    17401 aagtaatttc agagcataga taagataaaa atattaatta tacttaaaac tttgtggaaa
    17461 aggttatttc taataaattt ttaaatatat gaaaatgtaa ttaaataggt taaataggtt
    17521 taactgttta acttgttaga cctatttata ttttttagaa attctactcc ttttgatgtt
    17581 ataattgaac ctccacgacc aacaagtatg tctacaagcc caaagttttg tagttctaaa
    17641 agcatatttc taatctgttg ttctgtcaaa aatctatttt cctctaaagc tattttacat
    17701 aagctttttc tacctattct ttcttttaaa tcataagcct tttttaaatt atccaatata
    17761 aatatgtatt catctagtga ccttttaaaa ttatagtcgt atttcatgat gtttttgtct
    17821 ttgccaatgt tgaattgata cttttttata ttatcactta ctttattaca aacggttcta
    17881 ctataatttg aatctatagt atctaatata tattcaggta aatcacatat ttctataata
    17941 tctttttcca agtaacagaa gtattctcct aagtttctta attctcttac atttccttcc
    18001 catctataca ttttaaagat ttcttttact tcctcactta gaataaaatt acatggaata
    18061 tcatatttta atgaaccaaa aatttcaaat atatcagcag gtctttccct aagtggatgt
    18121 attttaaggg gaagaacatt caacctaaaa tataaatctc ttctaaacat atttttagat
    18181 acaagttctt ttaaatttct attagtagct gcaattattc ttacatcaat gtcaatgaca
    18241 ttattagaac caatacgtcg tacttgtttt tcttgtatga ctctaagtaa tttagattga
    18301 gaatttaaat ccatttctcc aatttcgtct aaaaatatag ttccattgtt ggctaattca
    18361 aataaaccaa ttttcccacc ttttttagca ccagtaaaag ccccttcatc atagccaaat
    18421 aattcacttt gcagtagatt ttcttgaaaa gtactacaat ttactgcgac aaaaggacca
    18481 tcttttctcc tagaagcact atgaatggat tgagcaaata attctttacc agtaccactt
    18541 tcaccaatta taaggataga tgaatttgat tgagccattt tattagcaat tttttttgta
    18601 ttaatagttt gtatactaga acctaatatg tcatcaaatg tgtatttact gatattacca
    18661 gagtttacta gttgtgctct taatttagct tgcttttttt ctaattgaga aaactttgtc
    18721 atctttagta taaatccatc aaatacattt aattgtatat gtttaatctc taaatttata
    18781 tcaatatgat ttattttaat caatttctca aaaaatggag ttttattggt caatatgctt
    18841 tgaaaattga tgtcttttat gcaatcacca acaaatgagt caatcatttc atttgcattt
    18901 atagacaaaa tttttctagc catatgatta tagaattgaa ttattccatc gttatttgtg
    18961 catataattc catcatcaat tatagatagc aacaaatcaa attgtctttc aaacttattt
    19021 gcatctaaca ataactcacc tgtactataa cttgtaggaa ttattttttc tacgtatttt
    19081 tttattaggt catcttttat tagatgtttt aatttggttt ttgtagcaat ttcaacaata
    19141 caacttaaat ctattattct gtattttaaa tctataactt cacaattttt aaacttaggc
    19201 aagatttttt cgccaggagt taggattaca gcatctgtgg gagtaaactc aatctctgga
    19261 tagcagggca ggatatttat attgtctatt cctaatctat gtattaaaga aattgtttct
    19321 attgccatat ctttacttaa gttatataca taaataattt tatctttttc taatttggat
    19381 atcatatcaa tactgttctt ttcaaatgtc aacttaggaa caatgatttg agcatcatag
    19441 tgagataatt ttactatttc atcatatttt aaataagcag ataggagtat aagtttttct
    19501 ttaaaaaact ttaaatttcc ctcttcaaat gagtataaat ttatctttag attatcacct
    19561 aagaattgcc ttatttgatt ttcataaatt tctccagcgt ctttttttaa acttacaata
    19621 gctaattcca tttgtttcat tttaacctct catacaaatt tgacttattt tagattacat
    19681 aataattaaa ttagattaaa taatattatt taattattat tataaattta ttatagacta
    19741 cacattgaag taaaagaagt agctaaaatt attcctttta tttttgtttt ttgtatctat
    19801 ttggtataat agtattagag cgtataataa tttaggcaaa attttttaag gaagactaga
    19861 ggagaatata aaatgaaaaa atgtctttac tgtaataggg atataaagtc accaaataat
    19921 aaattagcta taaaatatga tgacaatatg aatatatatc catgtcgtgc agaatgtaag
    19981 gaaaaaattg aagaatatat agcaaaaaaa cctacatata agtttataaa tatattagag
    20041 gttcttttag gtgtgggcgg aatattctgc tttttgagta aatggactat tgcagcccaa
    20101 gttgtacttg gaattgatgc actggttata tttttattcc cattggcagg atttaaaatg
    20161 aatggaaagt taagtatgga acaaactaag aatcaaagta ggtctattgc aatatcaatt
    20221 ttggcattta tagtaataat aactgtatta tattttaaac aagttggtaa gtagagcata
    20281 attgtataag acatcattag agtatgaggt gagtttatgg aagaaagaaa attagcacca
    20341 aacaatgaga gggaagctct cataaaaaga ttaaaaagaa tagaaggtca agttaagggt
    20401 atacaaaaga tggtggaaca agaacgttat tgtgttgata tattggttca aatatctgct
    20461 ataaggtctg ctataaatag agtaggcaca attatcttag agaatcatat aaaaggatgt
    20521 gtagcagaaa gtattaaaca tgatgatgct gataaaactg aagagatgat aaaagagctt
    20581 atggatacta taaataaatt tacaaaataa ggaggcggtt agttatgaat caacaaggat
    20641 ttattatgga aattgttgat gatagaactg caaagttaaa aatgcaaaga cattcagctt
    20701 gtgctgcttg tggaaaatgt gctactacaa attcagcaga gtcaaaagaa atattagtag
    20761 aagtagacaa cacaattggt gcaaaagttg gtgaccatgt agaagtgagt atggataata
    20821 tgaatgtatt aaaagcagct gtaatggctt atataatacc tcttatagca cttcttgtag
    20881 gaacaatagg gacttattat atattggggg caattggaat gacaactgga gttgaagcca
    20941 taagtggatt agttggaatt tgtcttacaa taatctgtta tttatactta aagaagaatg
    21001 acaaaaaatt tagagacagt agagaattta taccagtaat aacaagaata ttaattgacc
    21061 tttaagataa ttataattaa aaaatagcaa tgtacttttt aaataaatac tcaatacgta
    21121 tttgtagagt ttaagtacat tgctattttt atatttttat tttgattttc tgtaaggaat
    21181 aaagtttttt ctgattttta aaagattcaa tgttcttaca aaagaagtta caactaatac
    21241 accaagtgct attgcagcac ttagaaatag cgcatctgta ccttttgaca tagccaaaga
    21301 ataatttttc tgaatcaaag acaacattgt attatatagc ccaattcctg gtattagagg
    21361 aagaatacct ggaattacga acacaatagc tggttgcttt agttttcttg ctaaagtctc
    21421 actgcacgct gagaccaaag cagcagcgat aaaacctgca aaaactgcat ttgtagtaaa
    21481 attaaataaa taaacatata caatccaacc aattccgcca gttactccag ctggaagaag
    21541 tagatatagt ggagcattaa agaaaacagc aaatccagca gttgcaacac ctgaaaataa
    21601 aaaatgcaaa atcatagaca tatcagtcat tagaaaggac ctcctattct ataccatgta
    21661 tcaaggacaa aaccaactcc agaagcaatt gcaataataa tcatagcagc atcaaaagca
    21721 cgagaaattc ctgatattaa attccctgat attaaatctc taatagcttt tataaaagca
    21781 actcctggga gtagtggcat tatagcgccg actataagca ttttagggtc atctaaaatg
    21841 cctatttcaa ctagtagaac tcctgaaaat gcaattaaaa aagaagatac taaacaggaa
    21901 aatgcagata ttgcacttat cttcatcgtt ttatcataag taattgcagc aaagattgct
    21961 attataaacg taagtataaa atttaataca taattccctc caagcaaaca agcaaaacaa
    22021 gcagatgcaa ttcctgtaca agagtaaaat aaccataaag gataaggttt tacatcttgg
    22081 atttcataaa gacgtgcaat ggcattatca atatcatact ctttgtcact aacaaattct
    22141 cgagaaaaac tgtttaataa agaaatctta tttaagttta tacctcttga ttgaatcgtt
    22201 ttcataaacg aaaagccatc aaatttctca tctgaaataa ttattactgt aggagttgta
    22261 aaaatattta catgataaaa ccctcttgac ctgcatattc taaggacact gtcttcaacc
    22321 ctagatgttt cagctccatt ttttatcatc agttccccta caaatagagc tagttttaga
    22381 atatttttct tatattcaag ttcctcattt tgattcataa atacacctcc aaattaaaaa
    22441 acgtaatatt gaaattatat caaattttta agtaatattc cttaatatct atacaaatat
    22501 attatctttt ttttgtttca aaatagtcac atttaaatta tatccaatta aaagaattag
    22561 acaatattat ttattatatt ttaacttaaa aataaagaaa gtaactatgt taaatatgga
    22621 aaaatggcaa aacacttgac tattaattgg taaaggacta taataataaa ttatcatatt
    22681 gataaaaacg aaaattatga aatgtctgta aaaataaaat ttaaataata actaggagga
    22741 gtaatatgtt tgagaacttg aaaaaagcgg accctgagat ttatgaaagt atgaaaagag
    22801 agttaaaaag acaacaaaga aatattgaat taatagcatc agaaaacata gtatcagttc
    22861 cagtaatgga aactatggga agtcatctta caaataagta tgcagaaggt tactcaggca
    22921 aaagatatta tggtggatgt gaatacatag atgaagttga aactcttgca atagatagaa
    22981 tcaaaaaaat atttggagca gaacatgcta atgttcaacc tcactctgga gcaaatgcaa
    23041 atattggtgt atattttgct atgttaaaac caggtgatgt tgttatggga atgaatttat
    23101 ctcaaggtgg acatttaact catggtgctc cagttaatat ttctggtcaa tattataaat
    23161 tttatgaata tggtatagat aaagacagtg gacttataga ttttgatgaa gttagaaaga
    23221 tagcacatga agtaaaacct aaaatgatag ttgcaggagc aagtgcatat cctagagaaa
    23281 tagattttaa aaagtttaga gagatagctg atgaggttgg agcattgctt atggtagata
    23341 tggctcatat agcaggtctt gtggctgctg gtcttcacca aaatccatgt gaagtggctg
    23401 attttgttac aacaacaact cacaaaactt taagaggacc tcgagggggt gttattcttt
    23461 gtaaagaaaa atatgctaaa gacatagaca aagcaatatt ccctggtatt caaggtggac
    23521 ctcttgaaca tataattgct tctaaggcag tttgctttaa agaagcttta agtgatgagt
    23581 ttaaagaata tcaggtacaa gttgctaaaa atgcaaaagc cttagctgaa gaacttatta
    23641 aaagagattt taaattgatt tctggaggta cagataatca tttaatacta ttagatttga
    23701 ctaataaaaa tataactggt aaagcagcag aaaagagatt ggatgatgct tatataacag
    23761 ctaataaaaa tacaattcca tttgacccaa atggtgctct tattacaagt ggaataagac
    23821 ttggtactcc agctgtaact actagaggta tgaaagaaga agatatggca ataatcgctg
    23881 aggctataga cctatgttta acttatgatg aagaatctaa ggctagaaca ttggttgttg
    23941 gtttaactga aaaatatcct ttatatgaag aatataatat tatggattaa ttataaagaa
    24001 caactaaata aaatacattt aaataattac aatgattgtg tatgtatttg aatatagtta
    24061 gatataaaaa aggtgtctca aaatgaactt tttagtttat gaggcacttt tttgtatgga
    24121 atcgaacata tttgtgttat ttaaataata aaaaatatgg ttatctctat ttttagatgg
    24181 tctctttgta ttttttaatt aaaactttaa gatttcttaa gaattatact aggtttttct
    24241 aaagatttaa agtctatact tataagtata gtaatcatat gaaaagaaaa gctttaacga
    24301 ggagggaatc ttatgaaggt attaattctt acaggaaaat ttggtatggg tcattactca
    24361 gcatcaaatt ctttaagtga agatataaaa gcaaaatttg ataattcaga aataataatt
    24421 aaagatatat ttgagtacat tatgcctaac tattcagata aaatgtacaa gactttttct
    24481 attcttgtaa atcggggaag tagtctttat aatttatttt ataaatgtgc tgaaaatgga
    24541 aagaaagata taaaatttac tttttcagat tattttttaa ataaattaga tacattactt
    24601 catgaagtac aaccaacagt agttatttca acttttccat tttgttctca attagtatca
    24661 agatataagg aaaaatataa ctcaaatctt cctttaataa catgtataac tgatataagt
    24721 agtcactcag aatggattag taaaaataca gactgttatt tagtagctag taaatctact
    24781 aaggaagaac ttgtttttaa aggaatagat gaatctaaga ttaaagttaa tggtattcca
    24841 gtaaaaaaag aatttaaaag aatagaacat gtaaatcatt caactaaaaa aaatattctt
    24901 ataatgggtg gaggattagg attactgcct aaatcagaac aattttacaa agaattaaat
    24961 agtttagaag gagtaaaaac tactgtaata actggtaata ataaaaaaat gtactataaa
    25021 ctatatggta gatatgaaaa tatagaggta gtaggttaca ccaatgaagt atataaatat
    25081 atgaaagatt ctgaccttat tatttctaaa ccaggtggaa taacactatt tgaaacaatt
    25141 tattcagaac ttccaatatt ggcatttaat ccatttttac aacaagagat agataatgct
    25201 agttttatat taaataatga aattgggaga atacttggta aaaataaaaa atactatgta
    25261 gatgagataa aagatttaat ttatgatgat gctactctta aagaaatgag cagtaacatg
    25321 aaagaattaa aaaaacaatt tgataataat accttagaga atattttatt ttcacttgat
    25381 gaacaaggag cgtgtagaga atgtatgtag taggtttaat tatcgttgtt gcattgattt
    25441 ttttagtcca ttcaattata cctacatact ataataaact attaaataaa gaggtattaa
    25501 aaaatatggc tggagaaaat gaaattgcac ttacatttga tgatggtcca gataagagat
    25561 atacagaaaa attactagat gtgttaaaag aaaatgatat acaagctatg ttttttgtag
    25621 tagcaaagaa tgctgaaaaa gagcctgaaa ttataaaaag aatgttgaga gaaaatcata
    25681 tagttggttt acattcattg gaacacagaa atgcatggct ttatagttat agttatgtta
    25741 aaaaagattt tattgaaagt acaaatataa tgaaaaattt aggtgtagat gtaaactact
    25801 atcgtccacc atggggtcat actaatatat tttcaaattc atttgttaaa aaatataatc
    25861 taaaaatgac tttgtgggat gtaatggcag aagattggga gaaagatagt acagtagaca
    25921 ttattataaa caagttaatg agtagaacaa aagaaaattc tataatttgt ttacatgatg
    25981 caggtgaaaa ttcaggtggt gcagtaggtg cacctgagcg tactattgaa gctttaaaga
    26041 tagctattcc aaaacttaaa gccagtggtt taaagttcgt aacaccagaa aggatgtagt
    26101 tatgataagt aaaactgaaa agaaaaataa atgcctaggt agtgtagctt ttttagtttt
    26161 actaatgggc ataacaggat attttgtttt tagagggcag tcagttgaat cattgataaa
    26221 atcattaaaa ggtgcaagcc ctatgtttat actaattgga tttgcaatga tgtttatata
    26281 tgttgcctgt gaaggtataa atatatactt aggtatgaaa gctttaaacc aaaagactac
    26341 tcttttaaaa tgtatgggat atgcttttat tggattttat tttagttcta ttacaccttc
    26401 agcatcaggt ggtcaaccag cacaagttta ttatatgaaa aaagacgata taaatatatc
    26461 atattcttca cttatactat tagtcattgt tgttattcac caagtagtta tactggcata
    26521 tagtggaata atgttcatta tggaacgtga gtttatatta aataatgtaa gtggtatgaa
    26581 cattttacta atatatggtg taataacaaa tgttgctctt gtcattggag taattgcaat
    26641 tatattttca aaaaaacttg taaacaattt tataatatca ataacaaatt tattaggtaa
    26701 gttaagaata ataaaagatg ttgaaagttc aagaaaagtt ataatatctc aaattgaaga
    26761 gtatgttaaa ggtgctcaat atataaagca gaatccaaaa cttgtagtcc aaattttagt
    26821 cataactatt gtacaaataa cagctatgtt tttagttcct ttctttgtat acaaagcttt
    26881 tcatctatca acatatactg tatttgagat attagcgata caatccttgc ttaatattgc
    26941 tgtttcttca ttaccattgc caggggctgt tggagcttct gaaaatagtt ttatgacatt
    27001 atttaagata tttttccctg gtcacttatt agttccggca atgttactta gtaggggaat
    27061 aagtttttat gcatttgtag ctataagtgg attgatatgt atagtagtac atgtaaaatc
    27121 ttctgaaaaa gtaaaagcag tgaaaaaagt tgcttatgta agataatatt atatagatta
    27181 tttttattaa tatgtcaatt tgtgtgtata ttttaattaa gctttaaaca tatttacagg
    27241 agtagaaact attaaaacgt gtgacattgc taagcttttg ctaataaagt ataatatttt
    27301 atatagtttg aaatatttaa gaaatatagc aaaaaaagga gcttggtgat gaataaagag
    27361 attccaaaaa aaattttagt agctgatgat aatcctgaga ttagggaaat cgtagaaata
    27421 cttttaactg gtgaaggtta tgaagttatt atggcaacga atggagaaga agctgtaaac
    27481 ttagtagata gtacgattga tttgattatt ttggatgttg taatgcctgt aaaaacagga
    27541 tttgttgcat gtaatgaaat tagagaaaaa acgacagcac caatattatt tttaactgca
    27601 aaaactcaag actctgataa agttattgga ttttcagcag gaggagatga ctatttatca
    27661 aagccttttt catattctga actaatttca agagttaagt ctctacttag acgttattat
    27721 atataccagg gaaaggaaaa ggaaagagag ccaaatatat taaaaacaaa agagttatgt
    27781 gtcaatttag atactcaaga tgttacacta aatggagaac ctgtgttttt gacaactata
    27841 gaatatagta tattaattct tatgttgaaa aatagaaaaa aagttttttc gtctgaaaat
    27901 ttatatgaaa ctatttggga tgagcagtat ttttatacag caaataatac aataatggtt
    27961 catattagaa atttaagaaa aaagttagaa aaagaccaaa aaaatccaca atatataaaa
    28021 accgcatggg gaaaggggta ttatattgat taacaaaatt aagccatcta agttgggagt
    28081 tatactattg atatatgtag taatctcagc tgcagtggct ttcattttaa atataccatt
    28141 ttactctgct gttgaatcta tagtaaaaga tgtatcagaa gggaaagtat attttaaaag
    28201 agaagtaaat caacttatgg ataattttca aacttatgtg aataacaatg gtataagagc
    28261 agatgatgga gatgatatag caaaatggaa tagtgataat tggtttgttt ctatgaagat
    28321 atataaagga aataaggttt tttatgatac attatactat cctaatgaag tacctgataa
    28381 gagtgaggaa aatgagtttt atagcagtcc catggctaga aactatgaga tacaattttc
    28441 tgatattaaa gctaatatag ttgtagtagc tttatttaga tttagatttc aaaattttat
    28501 agaagcaatt tctattacat ttatggcact tacatttatt ataacatttt tactgttgtt
    28561 tagtagaaag ataaagtacc taaggaaaat agaaatgggt ataaagatag tagagagtgg
    28621 cagtttaaag tatagaatac cagtaaaagg taatgatgaa ttatcatcac ttgcatctag
    28681 tattaatgag atgagtcata cattggagaa tcaacttata aaagaaaggg agatgaagaa
    28741 taaaaataaa gatatagtaa cttcaatatc acatgatgtt agaactccat taacgtcagt
    28801 aatttgttat ttagatttga ttaaagacca taaatacaat agtcctgagc aattggaaca
    28861 atacataaaa aatgtaaggt ctaaagcata tcaaataaaa gatttaacag atgacctttt
    28921 tacgcatttt gttaattctg gagaggaagt ttcaaaaggg tatgaaaaaa taaatgctaa
    28981 tgaatttatt tttcaattac ttttagatag ttcatataca ttggaggaga aaggatttca
    29041 tataacaata gaaaatgaac tttataacca atatcatatt tatgtagatg ttacacaatt
    29101 tagaaggata tttgataatt tatgttctaa tataataaaa tatgctagga aagataagcc
    29161 tatagtattt ttaattaaag tagaggattc taaattacta attatacaaa aaaatagagt
    29221 aagtaatttt tctaagaatg tagaaagttt tggaattggt ataaagaatt gtgagaaaat
    29281 agttgcatat cataatggaa gcataaatgt aaattcagat gataaagaat ttgctattga
    29341 aatatctatt ccagcaaaag aatcataaaa atttaaatta tatattgtgt aattgctatt
    29401 atatattgtg taattgctta tgtaaaattc tatgaagcag gaagcatgtg actttagtta
    29461 tgggatgttc aatattgtgt acctctaggc aagtaacaag tttacttaca agaccatata
    29521 atattattat cttaaaaaaa ggggacataa taatatggtt ttgacaggag cacattatat
    29581 ttacattcta tttatggtaa ttatattaat tactatgatt atgaaaaagg atacaatagt
    29641 accatgtata ttaggtgttt tttttatggg gttgttcttt gaaaaaaaca tttttggtgc
    29701 tataacagca gtgtttaact cttttattat atctttaaat gagttgggtc caattatctt
    29761 gattatagct attatggttg cattatctaa agccctagaa gccaataatg ctatacagta
    29821 tatggtgaga ccattttcga gagttataaa aaattctaat acagcatttt ttgtaactgg
    29881 atttgttatg ttagttcttt cttggttttt ctggccaact ccagctgttg ccttagttgg
    29941 agctgtattt ttacctgttg ctatgagagc tgggctacca gcaattggtg ttgcagtagc
    30001 attaaattta tttggtcatg gtcttgcctt gtctacagac tttgttattc aaggagctcc
    30061 aagtataaca gctggtgcag caggtgttgc agtatcatat gttataaatg atggtatgat
    30121 tttgttttgg gtgatgggaa tcgtaaccat atctatggca ttttatacat taaaaagaga
    30181 tattaacaaa ggaatgttta gagaagaact aaaaggtttt gaaagtgaag aagtagaaga
    30241 gttcaatgga aaatctaaaa tagcaactat tttagtagct ctgggatttc ttgctgatat
    30301 aattgctatg tatatatttg acctaaaagg aggagatgca tcagctcttt tgggtggaac
    30361 agcagtattt ttaataataa ttataaatgt tataaatttt ggcaaagata gtcttgaaaa
    30421 tgtatgtgag aatataattg atggttttgt gtttgggata aaaatatttg gagcaattat
    30481 accaatagca gcatttttct atatgggtga agttgcacca ttgacaggtg tatttggaaa
    30541 agtattagca ccaggttcac aaggtttatt atcagatata ggcatagctc tttctcaaac
    30601 aataccactt aataaatttg ccgtatcagg tatagaaact gtagtgggag caataactgg
    30661 gcttgatggt tctggatttt ctgggatttc attagttggt tcacttgcct ctgtatttgg
    30721 aactgctata aatgcaagtg ttggtgcact agctgcatta ggacaaatca gtggtatatg
    30781 ggtaggtgga ggctgtctag ttccttgggg tcttatttca gcagcggcaa tttgtggagt
    30841 tagtccaata gaattggcaa agcgaaactt tgtcccagtt ataaccggat tggttgtaac
    30901 aaccatagta gcaatgttta ttatttaata aatcgaataa atctttaatt gtataaatta
    30961 aaaatagatg tgtagctata atttattata attgttttac taattctaag ccatacatct
    31021 atttttattg atatttttat tagtattctt taataaaata atatttttca ttatactata
    31081 cataacatct aaatataaca tctaaaatac tgttatctat atttaatatc ttgccaaatt
    31141 ttttgatgtt ttattgccct gtcccaccaa catatataag ctatcacaag tttttaccta
    31201 tactatcacc aaacaaaaat tctaatttta atttattatt ttataaccta tattatatta
    31261 actatattag tttgtagact ttagatgtaa ttataaatat taatactcca agagttcaaa
    31321 ttcatatcct agttttcttg catttgatat aaaatcattt aaaatttctg tactagtttt
    31381 ggaaactgca tgtagtaaca agatagaacc atcatgtaga ttatccatta ttttttgcgt
    31441 tgcttcttca tgtgatggtt gtttatcagt atcccagtct ctgtatgcaa aactccaaaa
    31501 tacagtcttg tatcctaaat ttttagtcat tgccaagctt ttttctgagt attttcccat
    31561 aggaggtcta aaaaatttaa ccatatcttt cccagttaca tctttatata acttttcaac
    31621 atcatataat tcgtcattaa agttttttag attgcttgtt tttgttggca tagatggatg
    31681 ggttttacta tgatttccaa ctatatgtcc ctcagaaacc attcttttta ctaaatcttt
    31741 attttctttt atatatggag ctgtaacaaa aaaaactgct ttaacctgat tttgttttag
    31801 agtgtcgagt atttttgtag tgtatccatt ttcataacct tcatcaaatg taagatatag
    31861 tgttttttga ccatcttttg taggtccatt gtatagggca tcatagtcag aaaatttaaa
    31921 ttcaatgtcc gtatttactt ctggtgtttt atgcttattg tttggtatat aaaaccaatc
    31981 aagagtagtg ttatctaaag aagatacatc taatttatta ttatcattat tagtagtggc
    32041 ttttgatggt ttatctgttc cgtctgaaac tattacattg gaagtatctt taccactatc
    32101 tattttttcc ttatcttctt gtaactgtgt ttccttattt tgattttcgt tttgattgtt
    32161 ttgtgaattt gaacatccaa caataaaaaa aatagaaata gaaagtagtg ttaaataatg
    32221 caatactttt ctaaacattt tgaagctcct ttctaaatta ataataattg catataataa
    32281 taattatatg attaattaca attgcatata gtattattat aattggaaat tactattaat
    32341 aatagttata tctttaaaaa taaggtcaca atagtatgac aaagggtgaa taatcaataa
    32401 atttaaaaaa aaatgaaaaa aatagttgac catattatga gaagatgtta ttataataaa
    32461 tgtgctcaac aagaagtaac aaaataacaa gcacaaagaa gttaattgaa tgaaatgaac
    32521 tttgaaaatt aaacagtagg ttaatttata aaacaagaaa caaaccataa agccagatat
    32581 tttgataaca atagtatctg agcctgataa acttttattt gagagtttga tcctggctca
    32641 ggatgaacgc tggcg
//
LOCUS       DAGSHT010000022        32630 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGSHT010000022 DAGSHT010000000
VERSION     DAGSHT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.023"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..129
                     /locus_tag="MJB72_000978"
     CDS             <1..129
                     /locus_tag="MJB72_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY8262103.1"
                     /translation="LNPGDSTFIKANVPHKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(324..1310)
                     /locus_tag="MJB72_000979"
     CDS             complement(324..1310)
                     /locus_tag="MJB72_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBY8262104.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(1340..2035)
                     /gene="pxpB"
                     /locus_tag="MJB72_000980"
     CDS             complement(1340..2035)
                     /gene="pxpB"
                     /locus_tag="MJB72_000980"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBY8262105.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(2055..3275)
                     /locus_tag="MJB72_000981"
     CDS             complement(2055..3275)
                     /locus_tag="MJB72_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBY8262106.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(3304..4095)
                     /locus_tag="MJB72_000982"
     CDS             complement(3304..4095)
                     /locus_tag="MJB72_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBY8262107.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            4683..5312
                     /locus_tag="MJB72_000983"
     CDS             4683..5312
                     /locus_tag="MJB72_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262108.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(5426..6940)
                     /gene="glpK"
                     /locus_tag="MJB72_000984"
     CDS             complement(5426..6940)
                     /gene="glpK"
                     /locus_tag="MJB72_000984"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBY8262109.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVDDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            7184..7726
                     /locus_tag="MJB72_000985"
     CDS             7184..7726
                     /locus_tag="MJB72_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8262110.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            7740..10232
                     /locus_tag="MJB72_000986"
     CDS             7740..10232
                     /locus_tag="MJB72_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8262111.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            10653..11051
                     /locus_tag="MJB72_000987"
     CDS             10653..11051
                     /locus_tag="MJB72_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8262112.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(11268..11477)
                     /locus_tag="MJB72_000988"
     CDS             complement(11268..11477)
                     /locus_tag="MJB72_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262113.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(11509..11718)
                     /locus_tag="MJB72_000989"
     CDS             complement(11509..11718)
                     /locus_tag="MJB72_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262114.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            12017..12436
                     /locus_tag="MJB72_000990"
     CDS             12017..12436
                     /locus_tag="MJB72_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262115.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            12664..13119
                     /locus_tag="MJB72_000991"
     CDS             12664..13119
                     /locus_tag="MJB72_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262116.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(13340..13600)
                     /locus_tag="MJB72_000992"
     CDS             complement(13340..13600)
                     /locus_tag="MJB72_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262117.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(13863..14216)
                     /locus_tag="MJB72_000993"
     CDS             complement(13863..14216)
                     /locus_tag="MJB72_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262118.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(14233..19554)
                     /locus_tag="MJB72_000994"
     CDS             complement(14233..19554)
                     /locus_tag="MJB72_000994"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262119.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(19571..20554)
                     /locus_tag="MJB72_000995"
     CDS             complement(19571..20554)
                     /locus_tag="MJB72_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY8262120.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(20555..21253)
                     /locus_tag="MJB72_000996"
     CDS             complement(20555..21253)
                     /locus_tag="MJB72_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBY8262121.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(21250..22302)
                     /locus_tag="MJB72_000997"
     CDS             complement(21250..22302)
                     /locus_tag="MJB72_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBY8262122.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(22295..22723)
                     /locus_tag="MJB72_000998"
     CDS             complement(22295..22723)
                     /locus_tag="MJB72_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY8262123.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(22723..23049)
                     /locus_tag="MJB72_000999"
     CDS             complement(22723..23049)
                     /locus_tag="MJB72_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBY8262124.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(23065..24594)
                     /locus_tag="MJB72_001000"
     CDS             complement(23065..24594)
                     /locus_tag="MJB72_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY8262125.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(24876..25298)
                     /locus_tag="MJB72_001001"
     CDS             complement(24876..25298)
                     /locus_tag="MJB72_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262126.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(25298..27751)
                     /locus_tag="MJB72_001002"
     CDS             complement(25298..27751)
                     /locus_tag="MJB72_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262127.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(27751..27918)
                     /locus_tag="MJB72_001003"
     CDS             complement(27751..27918)
                     /locus_tag="MJB72_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262128.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(27945..28391)
                     /locus_tag="MJB72_001004"
     CDS             complement(27945..28391)
                     /locus_tag="MJB72_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY8262129.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(28517..28945)
                     /locus_tag="MJB72_001005"
     CDS             complement(28517..28945)
                     /locus_tag="MJB72_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBY8262130.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(28959..30023)
                     /locus_tag="MJB72_001006"
     CDS             complement(28959..30023)
                     /locus_tag="MJB72_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBY8262131.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(30028..30471)
                     /locus_tag="MJB72_001007"
     CDS             complement(30028..30471)
                     /locus_tag="MJB72_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262132.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(30720..31160)
                     /locus_tag="MJB72_001008"
     CDS             complement(30720..31160)
                     /locus_tag="MJB72_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262133.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(31265..31462)
                     /locus_tag="MJB72_001009"
     CDS             complement(31265..31462)
                     /locus_tag="MJB72_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262134.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            31631..31951
                     /locus_tag="MJB72_001010"
     CDS             31631..31951
                     /locus_tag="MJB72_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY8262135.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            32033..32539
                     /locus_tag="MJB72_001011"
     CDS             32033..32539
                     /locus_tag="MJB72_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262136.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT        10686 a         5578 c         3612 g        12754 t
ORIGIN      
        1 ttaaatccag gtgactcaac atttataaaa gctaatgtac cacataaaat catcaatcca
       61 agtaaagata caatagctcg tggatatgga gttatatcac ctccaatttg gccaataaaa
      121 tctaaataaa ctcaatatga cacacctact ttttgtagat aatataatag atttaaatat
      181 tgcttttatt ttaaatttaa aatatttact caaactaaaa aaatagctat ttgactaatc
      241 taaaccatct taattatggt aaatagtaaa tagctatttg taagttatat tataaattat
      301 aataaaaatt gtatttgtta aaattaaact gctatatcat ttaatacttt tgcctttaca
      361 actcttatat ttttcatact cttgtagata tcttgaatct tatcttcttg ctctctcaat
      421 agtctatgtg cttcatatat atctacaagt tcaaatttta ctacatcatt tggctttgct
      481 tgtgctaact tatacaaatc tacacttata acgttcccta tcttagtata gccaccacta
      541 gtctgtctat ctgataacat tataataggt tttccatgac caggtacttg tatagctcca
      601 taagaaatac catcagatat aatatctgct ccattaaggt gctttatact ctctccatct
      661 agtctacaac ccattctatc tatttcattc gtaactttgt atttatttga taaaaatttc
      721 tctacttcac cgttagaaaa agcatcaaat tgaggacctt ttacaattct tagtacgatt
      781 tctgaactat aagttggaat atattttgaa cttaattttt tatttattgt aaaatctttt
      841 tcctgaataa aagtatttat ataatctcct tttttaagtg ctctaccttc aaatccacca
      901 attttagctc ttgtataagt tgacttactt cccattgcaa gtggaacatc tattccacca
      961 gctattgaca cataggctct agcacctgat ttcacacctc taaatgataa tacatcacct
     1021 ggatttattt gatatgacct ccagttttga atttcttttc catttatttt agcccctaaa
     1081 tctcccccag ttactgctat ttgcgtatat tcatcaaagg taattgtagg acctaacatt
     1141 aaaacttcta aggaagcctc atcttcagaa tttcctacta atatatttcc tactctatgt
     1201 gcatactcat ccattgctcc acatccagat acaccatatt gaccatagcc aactctacct
     1261 ctgtcttgta ttaatgtttg gaaacctcct aattcaactt tgaatcccat atataccact
     1321 ccctgtacta taacgatatt taaatactta agttattttt ttcgatttct ataaattcat
     1381 ctaatgttat tggaacaaat tttataaaat ctccagctct aagcaaaatc tgttcttctc
     1441 tattttcatc atataatttt aaaggtgtcc taccaattat ttgccatcca ccaggactat
     1501 caataggata tacaccagtc tgggaacctg ctattccaac actcccacca taaattttag
     1561 tccttggcac ttctaatcta ggtgtagcta ttctttcatc cataccacct agatatggaa
     1621 atccaggagt aaaccctagc atatatatca agtactctct gcttgtgtgt atttttataa
     1681 cttcatctac agtgagatta ttgtagcttg caacattttt aatatctata ccaaaaacct
     1741 catcataaca tactggaatc tcatgtattt taggttttgg taatactata ctttccaaat
     1801 tatcttctat tttttttaaa ttctcaatca aactatcaaa atctatttct aatggattat
     1861 aacttatcat aagtgaccta tatgttggta ccatttctgt tacaatacct ttaagattcg
     1921 atatttctat tgctctcata aatgaaatta cttttttgtt tatatcttct gatatctcat
     1981 ttccaaattc agcaaccact gccttatccc ctgatatcaa gtatctagtt tccataaatg
     2041 tcacctcttc taaattaagc aaataaagca cctaggctgc taagagaagt tattcctgta
     2101 tatgcagata ttaaaactac tattaatcca aatattaaaa gccatgtagg atgcttgtaa
     2161 tctccaacaa tatcttttct tttagatgct ataagcataa ttcctaatgt gattggaagt
     2221 atcaatccat ttaaagctcc tgctaaaatt aataaagtag ctggttttcc tataaatgcc
     2281 attataagtg ttgatattgc tataaatgct attataaaat atttttcata tttatctata
     2341 aaaggattta gtgtctttaa gaaagaaact gatgtataag cagctcctat aactgatgta
     2401 attgcagcag accataatac aagtccaaag aatttatatc ctattgctcc tgcactaaat
     2461 ttaaaagctg aagcagctgg attttctggg tcaagttgaa gaccttttga aaccactgcc
     2521 agtattgcta aaaataaaag aactctcatc attgtggcaa ctaatatacc caaaagagaa
     2581 gatttagtta tttcttttat attttcttct cctgttattc caccgtctat taatctatgt
     2641 cctccagcaa aagttatata acctccaaca cttcctccaa gtagagttat tattgggaat
     2701 atcagtcctt ttggattttc tggttttacc attcttgata ctgcctctcc aacaggagga
     2761 tgatttgata ttgctacata tccaactata attatcattc ctaatgctaa aaatttagtt
     2821 atcttatcga ctagagaatt tgaatttttt gacataaata caaatatggc tattaatcca
     2881 cttaatacag ttcctaatgt catattcata ttaaacatta cattcatacc aagtgcagct
     2941 ccaccaacat ttcctatatt aaaagctaat cctccaagag ctactaaaaa agctactaaa
     3001 taacctaatc ctggtattat tttatttgct atatcctgac ctctaagtcc tgatacacca
     3061 attactctcc atacatttac ttgagcccct ataaaaagga ttgttgtaat taaaattaca
     3121 aatgaaaaat tcggtccaaa atcttgggta aattgtgcag tctgagttaa aaaccctggt
     3181 ccaattgctg atgttgccat tataaatgca gcccctataa gtgctccgac atctttttta
     3241 tttttagttt tatctgtaat attttttgta ctcatatttt atctccccct catgttatca
     3301 gtcttatact atgttctctg acgaacaaac ttctatgttt tctaatggac aaacttctat
     3361 actttctaat tcaaaacgct ttcttatttc tttgacaaac tctattgctt ttggattgtc
     3421 cccatgtaca catatagaat ctgctttgat atgtataact tcacctgtta aagtttctac
     3481 agttccttct tttaccatcc taacaaccct atctattgca tgttttgtat catgtataac
     3541 tgctccttct acacttcttg gaaccaaaaa accattatta tcataagctc tatctgcaaa
     3601 tacttcattt gcaaacctaa gtccaatttc tttagcagaa tttatcattt cactattata
     3661 tagccccaac agtataatat ctttatcgac ttcatatata gcttcacaaa tccccatagc
     3721 taactcttta tctttagctg ccatattgta taatgctcca tgtggtttta catgttgtat
     3781 attacatcca tttgaacttg caaatgccat taaagcaccc aattgatatt ttgtataatc
     3841 tttagcttcc tgctttgtta ttttcatctc acgtcttcca aatcctatta aatctaggaa
     3901 acctggatgt gcgcctattt tcactccatt ttttttagct agctgtactg ttttttccat
     3961 atgactaggg tcacctgcat gaaacccaca agctatatta gctgatgaaa tatattttaa
     4021 aacttcttca tctaaaccaa tcttatacgt tccaaaactt tctcctaaat cactatttaa
     4081 atctacttta tacaatttat gacccctcct cgctaacatt ttgatagaat aagtagtttt
     4141 aatttttaaa ttttgtttat tttatatatt gcatgaaata ttttatcact tgtattcaat
     4201 tttatttttg caactattct attgcattta attaatataa ttttaccatt taaggaatat
     4261 ttttgcaatt attttaattg aacattcatt tgtatttctt tttcaaaaaa tctttttacc
     4321 attttttagt taataattaa ttattttcac ttaaaataag ggctttaacc ttcattttta
     4381 aagtattttt catttttaat cactttatat gcaagacaga atcatttttc ttcattcatt
     4441 tgtttctttc atctatataa ttatcattct ttgtaagtaa aatatcttta tatttttata
     4501 attaattctt tatatagttt taagtataat aaaatcatgt atactaaatg catgttaaaa
     4561 tattatttta aaacatgcac aatatttgta catattgtag acatataaac ctataaatta
     4621 tacaatagta ttgttaagat tatatatttt tttaacataa attttgtatt gggggaaaaa
     4681 caatggataa aaactatacg atgccaatat accaaaaaat agcgttggat attgcaaata
     4741 aaatatacac gggtgaaata caagaggatt cagtactatt tggacgctct gttttggctg
     4801 gtaaatacaa tgtatctcca gaaacgataa gaagagctgt caaaatttta gaggacattg
     4861 gcgttgttaa aagcataaag ggtaaaggtg ttattgtact atctccagac aaagcttctt
     4921 cttttataaa aaaatataga gatattacaa atatatcatc atataaatca acactttata
     4981 atttaataga tactaaatct aatttagaaa atgaaatatt agatactata aataaaattt
     5041 tagactattc taacagactt gaaataatca atcctttagt gcccgttcag ttcaccataa
     5101 attctaattg caaatatatt ggtcaaacag ctgcacaaac taaattttgg caaaatacag
     5161 gtgctacaat agttgcaatt aaaagaggtg aagaacttat aatatctcct ggaccatata
     5221 ttgaattttt agaaggagac attctcttag tagttggtga ccaacacatt tacaattcta
     5281 taccaatgtt tttatatgaa aacgaaaaat aaattatata atttagtatg attatataaa
     5341 aatgcaaaat taacaacaaa aaaagagctt catattttac ataaagctct ttttaaattc
     5401 aaataaattt aactttgatt aatttttaaa tatccaattc atcttctttt tcccagctta
     5461 aagctctcga tacagctttt ttccatcctt tatataattt acatctcttc tcttcactca
     5521 tatttggatt aaactctttt tctactgacc actttgaagt aatttcattt cttccaatat
     5581 agaatccaac agacaaccct gctaaataag cagctcctaa agctgtagtt tctactattt
     5641 ttggtctatc tatatctaca tttaatatat ctgattgaaa ttgcattaaa aaattattat
     5701 tacttgctcc accatcaact tttaatgatt ttagttttaa ttttgaatcg ttttgcattg
     5761 cttctaaaac atcctttgtc tgataagcaa ttgattccaa tgtggctctt actatatgtt
     5821 ctttttttgc tcctcttgta agtcctaaaa ttgaacccct agcatacata tcccaatatg
     5881 gagctccaag acctgtaaat gctggcacta catatacacc attagtatca tctaccctat
     5941 ttgcatacat ttcactatct tttgcagatt caattatctt taattcatct ctaagccatt
     6001 gaacagatgc tcctccaata aatatacttc cttctaatgc atatgttact tttccatcaa
     6061 ctccccaagc aattgttgta agtaacccat gcttagattt aactatgtta ctgcctgtat
     6121 tcataagcaa gaaacaaccc gttccataag tattttttgc agtaccttct tcaacacaat
     6181 tttgaccaaa taatgctgct tgttggtctc cagcacatcc cgcaataggt attttagctc
     6241 ctgataacat gcgctcatct gtatgaccat aaacatagct agaaggtttt actaatggca
     6301 acatgcttat tggtatgtcc aattctcgta atatatcctc atcccattct aaatcattta
     6361 tattgtacat cattgttcta gatgcatttg agtaatctgt tacatgtact tttccacgag
     6421 ttaaattcca tattaaccat gtatctatag ttccaaataa taactctcct tgttctgctt
     6481 tttccctagc tccttctaca ttatctaata tccattttac ttttgttgct gaaaagtatg
     6541 catctattaa aagacctgtt ttttctttta ttgattcttc caatccttta tttttaagtt
     6601 catcacatat ttctgacgtt ctcctacact gccatactat tgcgttataa ataggtttac
     6661 cagtatacct actccaaaca accgtagtct ctctttgatt agttatccct attgaagcaa
     6721 tttcttctgg tcttatacct gctgtttcta taacttctct cattacccca ctttgtgacc
     6781 cccatatttc catagggtca tgctctaccc atccagcttt tggataaatt tgattgaact
     6841 ctttttgtgc aattttcact atttcgcctt ttttattaaa tagtatagct cttgaactgg
     6901 ttgttccttg gtctaatgcc attacatact tcttttccat caccatattc tccttatcat
     6961 ataatattta atttattaaa aagaaaaatt taatatcaca tatttcaata aataatcata
     7021 cttaaataca aatttaatat aatagatatt tagtaagaag acacttattt tctatcatat
     7081 ctatataata atattataca ttatagctta catattgatt aataaatttt aaaggagtat
     7141 aataaacagt gttcataata attttaataa aagaggtggt aatatgccaa agattttaga
     7201 aaatgtaaaa gaagatatat taaaagtgtc tcgggatatg attttagaag aagattactc
     7261 gaatattagc ataaggaaaa tagctcaaag gtgtggtata agtgcaggga cagtgtataa
     7321 ttactttaat tcaaaacaag aaataataga atatatcaca aaatctgaat gggatttatt
     7381 gataaggcga attgaatact ccaataaaaa cactgaagat tacataaaaa aactaaacat
     7441 tatttttact gaaataagaa attttataaa caaagttcat aatatccaat acaatgactt
     7501 tttaaacact tttgaaacag agagattttt tgaaatgaaa aaacacaaag acgactttca
     7561 tgaacagctt tcaaataaag tatatgaagc tttagaaaaa gaaagttttt ttaataatga
     7621 caaattagta tgcaatatca taataaaaat gtttttttca tattcaacat atcttcatat
     7681 agaatttgaa gatttaaaac cctatattga aaaattaata cattaaggtg gtgtttacct
     7741 tgaatcataa tactagtatt aagaacaagg acagtaatcc aaaaatattt ttagtactta
     7801 cactttatct attaggtata tttatgggag ctattgatac tggaatagta tcaccagcaa
     7861 gaacgattat acaaaatagc cttggagtaa atgaaaaaac gggaatttgg atgattacca
     7921 tatacacatt aacatacgct tctgtaattc ctatctctgg aaaattagca gataaaatag
     7981 gaagaaaata tgtgtatcta gttagtatat ttctatttgg tagtggttca ttaatatgtg
     8041 gattatcatc actattttcc aatttctata ttttattaat tggtcgtgtt attcaagcaa
     8101 ttggtggtgg tggaatcatg ccaattgcta cagcagaatt tggaactacc ttcccagaaa
     8161 acaaaagagg catggcttta ggtttagttg gggcaacata tggtattgcc aatattttag
     8221 gttcaagcat tggtagcaca attctaagta tctttggaac tcaaaattgg aagtggctat
     8281 tttttgtaaa tcttccaatt tgtttaatca tacttattgg tggagttttc tgtattaaaa
     8341 acaataagtc tgaatctaaa gaaaaaatag ataagcttgg tacattgatg ttgatatgta
     8401 taatcgtatc actattatat ggtcttatga atatagactt ttttaatttt aaaaattcta
     8461 tacaagatat atcagtttat ccatacttat tgacatttat tgtacttata cctattttca
     8521 tatttattga aaatagagct aaagacccta ttttaagctt tgaatatttt ttaaatccga
     8581 gaatattgat agtacttatt ttatctttaa ttgttggtat tggaatgatg ggtatggtat
     8641 tcgttccaca gtatgctgaa aatgcattaa agataaatgc tggttctggg ggatattttg
     8701 tagctatatt gggattattt gcaggtattg ctgctccttt aagcggaaga cttattgaca
     8761 agtttggggc aaaaaaaata ctattactag gattttctgt atctatgata ggctctcttt
     8821 acctagtttt aattgctcta aaaaccaata ctgtatttag tgtatgtgta agtttaatat
     8881 ttatgggatt aggaatggga tttactatgg gaactcctct aaactatatg atgttatcaa
     8941 atacaagatt agaagaatct aattcagcac ttgcaacatt atccttgata cgctcaattg
     9001 gtacttcaat ttctccagct ataatgattg gatttatagc acatgcagga ttatctgtgc
     9061 aagataatat tatggactta gttggaaagc cctcaactcc taaaatagtt caacttgaag
     9121 aacttgaaaa catgatagat gacttaaaat cagaccctga aatggcaaaa aatttaaaaa
     9181 atgtctctat cccaaatatg aatgatactt caaatataaa tatggacatg acaggtggca
     9241 aacttccaaa atccttactt aataaagttc agtcagcaga tacaaccaac attaccaata
     9301 taacaaaaga aatatcaaca acaatgtttg atgaaaaagt tccatctata attactaaaa
     9361 tacagaaaaa tgtacaaaaa ggaatagatg gaacacaaag tggaataaac ggtattgaag
     9421 atggtgaaaa taaactaaat agtggtatta aaggagttca aactggtatt gacaatatgg
     9481 taaaagctag agctggtatt aatcaaggaa tagatggtgt aaaaaaaggt attgctggtg
     9541 ttgataaagg tataaaaggt atggaacaag gtcttgaaaa acaagatgag gccatcaagg
     9601 aacttacaac tgcttataat aaaataccta ctacagaaaa aactcctact gataacgaaa
     9661 ataactcaaa tgaacaaaac cagagtaata ccagtaattc tgaatcagac gacactaaga
     9721 atgattctac agaaaataag aataacaatt ccagcgagaa taaaccaaat gtaaatgaac
     9781 aaaaagaaag tttaaatgct caaatacagc aactaaaaaa gacaagagat gacttaaata
     9841 caaagttaca aaaaaataaa actcaaaaga acgaattaag taaaaaacta agatcaatgg
     9901 aatctcaaaa gaaagagcta caaagcaagt taaataattc tataagccaa aagaaagaca
     9961 tggaaaaaag ccttgataca atgcaacagc aaaaaaaatc actccaagca gttttagcaa
    10021 agacacaaga ggtaaaaact gaaataccaa aggcttttga taaatcaaaa ttggattata
    10081 taaattccat agaaaataat agaacaaaaa tagaaaacac actacaatct actctaaact
    10141 caggatttaa acagatgtat ataacagtat tctgtgtaaa tctactagca tttataatat
    10201 tattgttcta taaagaaaat aaaactagat aaaagagtgt tatagtatta tattgtaaat
    10261 ttatcttatt cttaaatatt ttatcctaaa gaataaattc ataaaatttt tgtaattgat
    10321 taatataaat tacaatataa tactattttt ctcttaatct ttttctcttt tacagacaat
    10381 caaaaagcaa tcttagttta tcgaaataaa tttatacaat ctattcatac acccctttac
    10441 atttatataa caattaaacc tattgttata tatacaaatc aaaataaacc atatattttc
    10501 aactataata tgttaaaatc atattatgag catattaatt taagcaaaat gtcatttaga
    10561 ctttaattta gtagttttat attataaatt tatatatttt taattttaaa tgtcatttag
    10621 acattactca taaaaagaaa ggagtgtatt ttgtggatac tttaggaaaa agaattgctt
    10681 atttgagaaa ctccaaaaaa cttactcagc gcaaacttat ggatatttta aaatttgaaa
    10741 acttaggcaa gtatgaaaca ggagatagaa aacctaattg tgatatacta atgtcaatag
    10801 ctgattattt taatgttacg actgactggc ttctctatgg taaagagaag gtaaatgtca
    10861 atagtagtgt aaaagaagat aaagaagatt acttacatgt tactaatgat gaaatgacga
    10921 tattaaatct atacagacaa ctaaacgaac gtgataaaat aaaaatagaa ggaatacttg
    10981 aacttaagat atctgaatat aaagacttaa aaaagcattc ctctaataat aatgaagata
    11041 aaatggttta aacatacata atttgatacg attttacatc atctattaac cttctttttt
    11101 tgtagtatat tactaaaata tggatattgg attatttaaa aacaagtata gtctaatttc
    11161 aagcacattt ataatattta gattatctta tccatatttt agtttaagta ctttgaaaaa
    11221 tataatacat ctctatgtat aacttatatt cctaagaaat gcattactca tgtttttaat
    11281 tcttccaaca tctttctata cacaattgta catccttcat aattcccctc tttaagaagg
    11341 attgaacatg tactagaata tttagttaaa gtatttaatc tttccatatt taagcttttt
    11401 atattactga gtgctgtaat aaaatcatca attgctctac aacactctat aaccccatct
    11461 gaatccaatt tacacacaag aatattcacc tactttatat actatttatt aatcatcttc
    11521 tgaatattct atattaagta attgataaat tgatgattta tattttttac ctgttcttct
    11581 tccatttaaa atagctgaca aatagttttc attaacacct attttcttcg ctaattccct
    11641 ttgagtcata tttaattcaa taagtctttg cttgacttgc actcccaaaa aaggtatcca
    11701 tttttttcga gcatccattt atcaacttcc tttctaagtg atttatattt acttttatat
    11761 tattttagct tacacacccc ctctatttag tatatatttt taatatgtac acatattaag
    11821 tatataaatt tatttaatac taaataagat tttttgatta ctttgtgcta aaattaggat
    11881 gtaaattcgt atcattaagt taattataaa gtcattttga ccttaagtca atgcttttta
    11941 tgtcattttg accttaaatt tgaaactatt tacatcttaa aaagtctttt tgacattaat
    12001 tgaatggaga gtgtttatgg atactttagg agaaagaatt gtttatttga gaaaagcaaa
    12061 aaaccttaaa caatatgaac tagaagaaat gttgggctgt gacaacttaa gtaaatttga
    12121 gagaaacatt agaaaaccaa attatgaaat attaaaatct atagctgaga tatttaatgt
    12181 ttcagtagac tggctattaa atggagataa tctatcacat aaatcagatt taatttgcga
    12241 ttcctcctca aactatccac ttaatagcat aaactctaat gaaataaagc ttttaaacaa
    12301 ttttagaaag ttaaatgatt atgataaagc caaaatagaa ggtatgattg aattaaaact
    12361 tcatgaatat gaaaaagaga aggatttagg aaaaattgag tataacaaaa ataaagatga
    12421 gaaaatagat aaataatttt tatgttgctt ttgatacgat tttacatcat ttctttgttc
    12481 taatattttt atttcattta ttttcttttt tataatttta tgataaaata attagtatat
    12541 tttgctttac ttgaatgtaa ttatttatac aaaagtcaaa cataatataa atgtagtaaa
    12601 tttttagttt caagtaatat ctcgttatta taactaaata tttattaaaa ttaggaggat
    12661 aaaatgggct taagagacaa attcgcgcaa tcatttgcta gatcaaaaac tatgagtggc
    12721 cctgagaaaa aggctaatga aataatgggt aaattgcttc ttaagaaagc tatattacct
    12781 atcgttttaa tgttcgtgat aataatcgct ggagctatgc ttaaaatcaa ttcttgggtt
    12841 acacttggca taaatttagt tattgctgtt ggtgctttct tctacataag aaattctagt
    12901 aaaaagtatc agaactttaa accttatgtt ggcaatttga ttagtttaga gaaaaaaggt
    12961 aaaaaagagt acgttgctat tataaaacaa ggtaagttac cagttaagtt acaaatagct
    13021 tatggtggtg aagaccttga acatgttaag aaaaatcaaa tggttcaaat aagttataat
    13081 ccagatgcta aaatagctat tttggttaat agacaataat ttaataaaaa aaaggagttg
    13141 tcttaaaatt gattttttct aatcaaattt aagctcaact ccttttttaa tttttatata
    13201 ttatttttat atattatttt tattactttt tttaattaat atttttaaat atcaaatcct
    13261 tctcaataaa atagggtact atctacaata ctactttatc ttttcctaac aatatataat
    13321 ctggtgctac tctttcatgt tatacaccgt tttgtaacat accttttata ccagtaacat
    13381 cttccttaat ttcctctaca tctgttttca ttatacccat ttgaacaagt atatttttgt
    13441 ttatttcttg ttgttgtgta gataattcta taaaattttc tacagttttt ttatacatgt
    13501 ctctatcttc ttttttctcc tgcatagtat ttttaaataa tagagcacat agtataccta
    13561 ttgcccctag actactcaat tcggttatta atttatccat tatagcctcc tattgtctaa
    13621 attttttata cttatagctt taatttttgt tatattctta catcatgcaa tattatattt
    13681 tcaggattat caatttaatc ttctgtactt taaattgtag gtgcaattta tagattgaat
    13741 aaataataaa tatatataca atttctaata taacaaaaaa taagtacaag gcttacttat
    13801 taaagttaag accttgtact ttacataaaa aaaataacta atacatagtt attctaaatt
    13861 tactattcaa ctggtatttc taattctttg catctaattc taacttgctc tctaattact
    13921 gctggtactt cttctattgt ttttttgcct cttaaaatta aagctacata tacttctacc
    13981 attccactct cacctccaga ttttcttaca ctaaaagagc ttatattact tgcttcattg
    14041 acagaacttt gatttaacaa catctcatat atttctgcat ttgctataaa attatttgtt
    14101 atatcatcat cttgctttac ttgacctata cttatgtctg aaatgtcttt atactgcatt
    14161 tctacttttc taccttgttt gatttgttct attttatttt tatcgaattt aaacaatttt
    14221 cacacctcca ttttaagctt gtggctcgtt gctttcagca tcatatctaa aaatcataga
    14281 tggacgtttt atagctgtcc atttatctcc tttttctact atgattttag gctgagcact
    14341 ttttatgttg gcttcttcta attcatagat ttcttggtaa cttgagtctg attttttgat
    14401 ttcaactagt ttcatgtctt tgaataagtc agatttattt atttttactt gaactttagt
    14461 tttatttatt aattgatata taggattgtt ttccatcatg tcatccttta atccctcatt
    14521 taatgttact attaaatcag tgttattttc agtaatgttt atgattgaat atactttatt
    14581 gtttatcatt aaaatagaat ccttttttac tttaagccct gtttctgatt taaatataaa
    14641 catacttgaa tataaatcgc ctgtaggact actggcttta atcagtatct tattttctcc
    14701 taatactatt ttattttgtg gtatctcaaa tataatgcta tttatttcca aattagtctt
    14761 agtagatatt aattcatcat ttataaacat ttcaagtttt tctataacat tattagggtc
    14821 ttgtactaca actctcactt gagatattga tttttcaact atttctatag atgctttact
    14881 tgtatctact tttgttatca tgtatattgt atcatctgaa agctctatat ctaaaatatc
    14941 atttagatgt tcaggaattg gtattttagt aaattcatat aaatcagtaa ttgatataga
    15001 tattttatac atagatttat ttttaccagt tatccataga ctattatcat ttttaattac
    15061 tagtatgtgg tcatcattat gtgacttaat ttctttaaca ttagttaata ctttagtaaa
    15121 agttcttatt atcttatctt tttcactttc tcctagtcca aataatccat ttgtattgta
    15181 cccttgagcg tacaactctc cctcattagt taaaacaagt gtcattgagt tatatatact
    15241 tatatcttta acagaaggta atccagtaac cttttgtaac tctcttacat cattattgtg
    15301 gccaactccc aattggtact cagtattctt tccatatgac catagagtat tatctgtctt
    15361 aataatatag gagtagttat ctccacaagc aacttttttg acatctgata tatttacttc
    15421 agtaagagtt aataattcta tatcttcaag ggtttgatta atttctttat atgaattact
    15481 tcctattcca tataacttgc catccttagc aacagccaat gaatgtgttt ttgatatatc
    15541 aatatcagat acctcaaatg gcaatattat tgaagtatac ttaaaattat ctttaaatat
    15601 gtttttataa tttatatact tattatttgt ttttatatac ttatcattaa acattactag
    15661 cttttcattt ttcttattaa aaactatttt atctgatgtg ttatctgttt taaaatatga
    15721 atgtagggaa ccttcaagag cttcattttt atcattatca tatctatggt gaatacctaa
    15781 atgattatat ctattatctc ctgttgaaga ataaaatttt ccatatatat ctttaataaa
    15841 tatagtattg taactagaaa atacagtatc aacatctttc tttatagata ctttataata
    15901 tggaactgac atatccctat aagattcatc agatatacca taagtatctc tacctgatga
    15961 tatcaaattg ttattattat ctatataaag tgtattatta gctgttacaa aaacatcttt
    16021 aacatcttcc acactatttg tattattatt atttgctata cttccatatc cactaagacc
    16081 tccaacaaat agtttattgt catttgttaa aatggctgaa gtatatccat taactactat
    16141 tttttttgca tttataatat tattaattac tcgtataccc tttatatcat tagtttctgt
    16201 tgaaaagaat accctctctt ttttccctgt agtataaata acattgtcat ttgttataaa
    16261 aaatgtagct tcacttcctg ctgttacaac agattttaca ttctcaatat ttatttttgt
    16321 aaatgtactt atacttcctt cagtaaaccc aaaatatcca tttacaaatc cacatacata
    16381 acatgtatta tcatttttaa caattattga tacatcatta catatataca catctctaac
    16441 tccatttata ggtatacgtg taaatatatt cctacttgta gtatcaccca atcctaactg
    16501 tccttttcca ttattacctg ctccatataa aagaccatca ttagttaata atagagatgt
    16561 gtttccatat acagctacat attttacatt ttcaacattt attttagtaa atacatttct
    16621 atttacatta tctcctattc ctaactgacc agaattattt aaaccaacag cataacaggt
    16681 attatcattt tttatcgcaa atacatgttt actattgtca gtaaatctat ttacatttat
    16741 agattttata ttatcaatat ttattttagt aaataccttt ctatttatct catctcccac
    16801 tcctaattga cctacatcat ttaaaccaac actgtaaagc atattattat tagttaataa
    16861 atatgtagac ttcgaatggc tatatatttc ttgtatatta gaagatattt cactactgct
    16921 attcttttta ttgtaattta tcttattatt taaatttaaa ctactattaa ctgatatacc
    16981 atgcgtaccc ttagagttat atccacatat aaataagctc ttatcagtta atattgcaaa
    17041 tctagtttta tctctactta aatcataagg aggtataaat atatttttta tatttttcat
    17101 taccttttta taattagtgg cattatatcc atcttgtgta tctgaaacat ctagttcttt
    17161 tgcactttca gatatattcc caaaaccata taacgtgtta gaagataaat acaatagagc
    17221 tttatataaa aatatggctt tatcagaggt aataggacct gaatttatat tagatgctgc
    17281 aatattatta gatgattgat tgttaagata attccttcca tattgatgta tataagatga
    17341 acctccagaa ataagtaaat tatctggatt tacatttttt aaattacttg ttgattctcc
    17401 ataatacaaa taggcaaaat ttgtagaata tgatgattgt gtacaccttg tgggataata
    17461 gttatatatg taattactaa tatatccagc atgtgtaatt ccttgttgtt gaactctata
    17521 agtagaatca cttggctttg tccataaagt accatggtat ccagttaaaa aagaaccata
    17581 atttgtacat aatatagcca tatattcata tataataata ttattaattt tagtaatatc
    17641 atttgtacaa gaatttgtac tataacttgc tcctgaagga tttacataaa accagttatt
    17701 tattaatgtt aaatttatat tgactccatc attaggatta acacccaata aatagtttgt
    17761 atttatacca gttgcatata cctttttttc gtcagccaca ataaatgttg cattatatga
    17821 actatatgca tctgttacat tgctaacacc aatctcagta tattgacttg ctgatttatt
    17881 actaagtcca ttaacataac ttgaactctt tccagaagta tatatttgct tattattagt
    17941 tacaattaat gaaaaccagt ttgataaatc agtatttata acaacttttt ttacattatc
    18001 tatagatatt ttcttaaata tatctgaatt agaaggaaga acacctgcta ttgagttact
    18061 atttggccca gaataataac aagtgtcatc atttttaatg tagaatattc ctccagaaat
    18121 attttttgat gctactatag actttacatt agagatactt aattttgtca actcatttaa
    18181 ttgacttggt aagtcagaac ccattccttt gtatctatta tttcctagac catatacatc
    18241 tccattccct aaaataacca gagtacaata aggactaaaa acgacatctc ttacagaatg
    18301 tatatctagt ggaagttcaa tatcctttat ttccatttct tcttttaata aagttaattt
    18361 atcctcagat ttaattaaat ttatataatt actataacta tatttttcac tatccatttt
    18421 cttaaattct acaatgtgca tatcatcaaa aaacgaacct aattgtccat aagtattatc
    18481 tcctgtacaa tatgaatcat tatttgaatc aattagtatg ctatgtgata aaccaatgaa
    18541 aatattctta gctacaatat tttttaaatc tataaatgaa ttatctatat tctcagagta
    18601 atttgctata cctaactgat atatatcatt atttccagca accttcaaaa tacctttatt
    18661 atctataagc atagtattac cattaccaca tgcaatagtt tttatattta cttcttctat
    18721 cttagtaaat agtgatgatt gaagtgcatt tccaaaacat ttttcctttc cacaaccata
    18781 tatatcatta ttatacgtca ttaacacagt gtgttcagaa ccacaagcta tagtcttaac
    18841 atcattaata gaaactaagc taaataaatc tctattattc ttatctccta aacctagttg
    18901 accttgatta ttaaaaccag tagaataaca tttattgtca ttctttatta taaaagctga
    18961 tttattatat gagaatacag cctttacatt atcaacatct acttttgtaa atgtatttac
    19021 atttgcagat gctccattag ttcctagacc taattgacca tacaaattat ttccagcaac
    19081 atataaagtt ccatcattgt ataaaagata tgttgattga agtccacatg caatatcctt
    19141 tacgcctttt atttctggaa ccttctcaaa tgtggaaact gtttttccta ctttttctaa
    19201 tcccaatccc atttgaccat ctgtatttat acctgcgaca agtaattctc catctgaagt
    19261 aagtataact gcatgagatt ctccacatgc aactttttta atattagtag atatatttgg
    19321 tattttcgta aataattttc ttgttgtatt gtcttctccc aatccaagtt gaaagttact
    19381 attatctcca catccataca agatattatt attttttaga ccaaatgtat agttacaaca
    19441 tccagtaaaa aaattaataa agtcataatt atcataccct tcatcaaatt catcaactac
    19501 catattttta tcgccaaaga aattacctct ttgaagtagt ttagttcttt tcattataaa
    19561 aacctcctaa ttatcttatt tcatacgaat atttatttaa agttatatct gtctggccaa
    19621 ttgaatatgc cctattagat tttgtttcaa caaagttatt atccatttca tgtatatatc
    19681 taactctatt tgcattaaat ggtagaacca cattcttaaa tgtaacacta ccaagttctc
    19741 cagttataca ctttctattt aaatctaact taaggccttc tatagctatt atgtttttag
    19801 cattagttaa ggtatcatac caatatccag cttcatctac atctatttct gactctaatt
    19861 ctaagtatat taaaatatct aatacttttt cagataattt attgtaacta ttttttaata
    19921 cagatagttc agtttgtatt tgacctccta ttacatcatc aatagatata tttttaattt
    19981 tttctactac ctcatttata gctcctgata agtttttagc ttctgtagat aagagttctt
    20041 ctccaatttt tacttttatt tcttttatat ttttatcaac cttatcatct aaatcttgaa
    20101 tatccttttg agttacaaaa accaacgtcg ggtctacttc taatgtaaca tttgaagtat
    20161 tggacaattg taaaatcatt tttacaacca aatctttaat actgccatct tcagcacgtg
    20221 gcttatacgt ctctggatac ttacctatag ctattatatt attctcatta tccagaacag
    20281 cagcttctct tatcataaat ccaccaactg ttcctggtaa aatagtctct ataactatcc
    20341 aattaggatt tttttcatca attcttagag aatttatatt tccttcccaa actacattct
    20401 ttaaagcagt ttgttcctct gttggctcat aatattctcc attactatct ccaacttgaa
    20461 acttggttaa tccaacaagc tctcctagtg cagttgcatt tgctattttt tctctaccaa
    20521 ttttggttaa tattatataa aatttttctt ccaattatac cacctccgat ttaggataaa
    20581 ctactacatt ttctacgcct gtatcatttg caatagctat attaatctta caattatttt
    20641 caatgcattt aatgcttaaa ggataaacct ttatttcttc tctacttaac attccagctc
    20701 cacagtttac aacagcgcta cattttataa ttggttcaaa gtatggatga acttttactt
    20761 cttcactaca taccatacct ccaccacaat agaccatact tttatctagt attattggtt
    20821 ctaaatagtg taataagtga gcaggtttta ctctttcaat catatcgcta caatctaaaa
    20881 gagttttata gtcacaatta tttgctataa aactcaatac gaatgtaaat tcatcactat
    20941 aaacttttat atctgtttct gattttgtat atgcctcaca tatacttttt ataacttcaa
    21001 tagtactagt acctctgctt ctcattttag cttttatatt gctacgtctt gtgtcaaaat
    21061 caagttcttt tttttcaatg cagagtatgt cttcccacat atctaatccc catgttgcag
    21121 tgtcaacaaa taattggtta aaggtatcat caatatcttc tttaagaata tttaattctg
    21181 tatcgtatgc accttgtatc tcctctacaa tgtaatttct atcaaatgat ggtagtttat
    21241 caattagctt cattgatttt ccacctcact aaaattaaca gttgttacac taggtatttt
    21301 atcttcatta atagttatat tatctgtact tccatttata agtaggttac ttaaatcgtg
    21361 tacaccagta gtatttataa gtattcccat tacttttgta tagattatct ctcctctaat
    21421 atctctaaag tatgtattta tactttctag gaaagattct tttacattgt ctaatgtata
    21481 tccatcttct aatttcatta ctgcacttat atttatttct attggtaatg gtgtcacaac
    21541 tgttatagtt ggtccaatag gcttctcttc atctatatgt tgctgacacc tttcaattgt
    21601 ttctgtatca acagcttgat tatttttccc aaatatcaag accttaactg ttcccggacc
    21661 atcccatctt ggataaacct taacattata gactccatct acttccaaag cccattcttc
    21721 atagtgagct ttatttccac ttgtagcttg atttctttgg attttataaa atctttcttt
    21781 tagttcttca tctgtctcta tatctgtacc accttcaaaa cttcttgtgt tagtaatctt
    21841 tgttactcca gatatattat caactagttt aaattcacaa tttgtaggta aattatattt
    21901 cttaccaact tccagagctt gaactgggct attgtcacct tcttcactac caatagttac
    21961 atcttttatt actacaaata gtaaatctct atatgatatt attgtgccat tatttattac
    22021 agttcctttc tctccaataa attcaacttc tccatttgac tctgtaccta actttctata
    22081 tacaccaaat tcattgactc ttttatcgag aaactggtta tatgtgtctt gtataaaagc
    22141 cattttatgt atctttgaaa gttctagata tatctttgaa agttctagat tatttccaga
    22201 taccatgttg tttagaaaag aaccttctcc tttataaata tcaagattaa tattttcaag
    22261 agttctattt tttattactt catatgtctg gtcactatac atttatttcc acctccccat
    22321 agatagtgga tacttttaca tttgcactca atacgtctct gttaaagtta gcacttttta
    22381 cgtttacttc taatatatat ggatttatta gaagggcctc ttttatgaat ctactagctt
    22441 cactttctgt aagacctttt gtatattttt gtcctattag ttctgaaagc tctgtcccat
    22501 attctaaaga gtatatctca tgttcatatc tatttgtctt tatacacttg tatatccaaa
    22561 cttttaaggc ttcttttttt tcaattattt taaagtcacc tttttctaaa ataggttcat
    22621 ctttttcaaa atcccatgcc acttcacgaa agattggcaa ttcttctgtt ttaggtaaga
    22681 tataatcctc tgggacacct ataaaaggaa atattgtact cattataagc tcaccaactt
    22741 acttacaaca gcaaattttt cgcctattct aaacattatt accatatccc cagattcaaa
    22801 attatctata aaaggatttt ttacttcatg ctggtgttct tgatttgttt ctgtgtcaaa
    22861 tgtttctagc tgtctatcaa tcatccaact atctatcaaa atatcttctt tttctaatat
    22921 gatgttattt gtctctattt ttaaatctgg taatttactt ttaatttttc caataaaaaa
    22981 agaaggttga ttatgatact ttccttcttc tcttattatt cctataaatt catttattgg
    23041 attagccatt atatcaccat ccttttataa tactctccta gctgtattaa aatctcttct
    23101 actacttaac ttacttattt taactacatc acctttttgt ggagcatgta aaaattctcc
    23161 attacctata tataatccaa catggctgac tggattatga aaaaatacta aatctcctgc
    23221 ttgaagattt ttttgttcta ctttcttgcc ttttttagat tgttgatttg acgttcttgg
    23281 caaactaaca ttaacttttt taaaacaata gaccattaaa ccagaacagt caaaactact
    23341 tggtccattt ccaccccatt tataaggttt acctagatgt ttttttgctt ctgatattac
    23401 tattttagct ttatcagata cattaccaga ataaccacca ataataattt ttccttttct
    23461 tcttccaaat ttactagctt cttcaacatt tccaaataaa atatctatat ggtatgttcc
    23521 attcttttct atagttattg caggtcctac gtcatttact ttatagactc catcacgacc
    23581 agaaactcct gtaattacct gtatttcatc tccatagttt aataaaggat gtttatttaa
    23641 aaactctttt gtataataag tttgttcata tttaccaacc ataggagcag cgcaagtttt
    23701 tttagatggg tcaagttttt tctttctaca atctgtatct ccaccttctt cttttctagg
    23761 acagtaagct gtaaattctg ctgtaaactc ttttcctcct gcataatctt ctccccctaa
    23821 gttactttct tccttaggtt catcctgtcc tgctgacttt tcatccataa gattttgaaa
    23881 attaagttca agctcaattt gatattctcc attttgccaa gtatgtttgt ctgtatctat
    23941 ataaaatagt cctacaagct ttgtataaga atctttaact tttactcctc taccagttat
    24001 acaacttaca tctccataac ctttaagaga acagcttttt tctatcccat taaactcgct
    24061 atcaatatct acatcttgat tttcttgttg ctgaattact ttttgcatta ttacatttac
    24121 ttccttaaaa atttcattat ctattttttc gctaatcttg cttccatact ggtctaccac
    24181 tattacttta ttttttacat tttccatgct ctccgaaaag gtggtattta taatattaaa
    24241 tccctcttca aacataacat ttaatgtaac agttcctttt tcaataacat taaacttatc
    24301 taaattggcc tctatcatat actttttctt tgtcttttta cttgcttctg tatatgcact
    24361 cattattgtg tcataaccat ttactcctat aaacatcttt gtatacttga cattggtctt
    24421 tgctattgtt ccaactgaaa gcctattttc agcaaatact tgctttgcta tgtcttcaac
    24481 taacttatct ttaaaattgt atgacacttc actttgtgta agtaaaaatc ccatatcttt
    24541 agatacaaaa ctaatttcat tactgcttga atctttagac ctattaatta tcattcctct
    24601 aaagagttct ttctcatcta catagaaaca gactgtacta gctattggta tatctatttg
    24661 ttggaaattt acatcaaatg atgattgtat tattgaaaac tctagtgtcc ttgatggaga
    24721 tttataatca cctgaccatg ataccttgtc tactatgtca gttatgtcat atatacttcc
    24781 gttttttatg tgtacccaaa tcttaataat aaaaaatcac ctgccttttc atttaattct
    24841 ataagcagtt atttataact tattatctat aatagttatt gttttggagt tacttttggt
    24901 atctttattc ttttatactc ttttaaatct aatgtaaaat atacatctcg agtcccatct
    24961 ctttcttcat aactaaaccc ttcaattatg acttccatat ttatatttgt ttccgtaatt
    25021 gtaaatctta atataaaacc ttccttcatc catttttcta tcttatttac acaatcatat
    25081 ggtgatggaa aacctgtata gtcacagaaa gtatagtctt tatttggaaa aaaacttgat
    25141 attgaaatgc ttttaagagc tacaccacca aaagttgcta tttcacctat tttgagtata
    25201 ctagaagagt ttacagcagc ttttccatta atactaaagg aagatggaaa tactggaaat
    25261 ctaaatctat cttcagcttg tctaagccac atttccatta taagacaccc cctaactgtg
    25321 ccaattggat acttgcaact aattttgatg ttatcttttc tatatcagct tcttctctaa
    25381 tcactattgt atcagctaac ttagctatgt ttatatttcc accatttttt ctgcttctat
    25441 attgatttgc ttcttgttta gttaaaacca tttcaccttc atggagtctg gtttgatagt
    25501 cattataagg aacatagttc aatccaaatg cattattacc tttcttacct gaataagctt
    25561 taccagaatc accagccttt gttccagcat atggattatc tttgtaattt tttttactct
    25621 tttccactag atttaaaact gttcctgtga tgcttccatt ttttatctta ttaatcatag
    25681 tttgccacca cttgcatata tctttaacta ttttacatac tttatctaat atgttaaaaa
    25741 aagctcccaa tattggctct ataattttcc atccagtttc caacagaggt cctaaggtct
    25801 cccatacagt tttccaaata acacccagtg tctctataaa attgtttatt tgaggtgcat
    25861 attcacctaa aaaagtaaaa attcttgtga taataggact aacagcttca actacaggtc
    25921 cgattagttg gaatgctgta gttactattg taccaaatat acttattaat ccttgtgcaa
    25981 ttggtgattt attgtttata ctatctagta aattatttac taagtttact actggaagta
    26041 atgaatctat taatgctgta aaatcgatat tttgtaatag tgtacttcct gcgttaacaa
    26101 ttgcttttgg tgcattccct atcattgttg ctgcatgatt ttcaaaacgt tcttgcttat
    26161 tttcaaccat cattttacgc ttttcagcct cattagaaat tcgatattga gtagaatcag
    26221 aaaactcttt tccatttttt acacttctag attgcatctg gtcatatttg ttgccaataa
    26281 ttatcttacc attcattaca ttgcctattt taccagccat tttttcagat aagccagcta
    26341 aattgcctaa tccactttct ataaaagttt ttattttctt tagtccttga cctaaaatat
    26401 catcaaaagg tttaacaata ctcttcaagc cattgttaat gcttgtttga gctcctgata
    26461 ttatacctgg tattgtatta ctcatttctt tagctaaccc catctgcttt acacttatgc
    26521 cttccaatgc ttcatcaaaa gttttataat tttctcctac cagagtatta agagattcca
    26581 tgtttccttt tgctgcactt aagaaagctg ctgatacatc ttgttcacta cttgtattca
    26641 tatttaaagc tctaacatct accatagctt gagttatatc tttttctttt ttgccaccag
    26701 taatattact aatcatttta gctttagttc ccattgcaac aacatcttca gggtcaaaag
    26761 gcgtatcatt tgccattctt actaaactgc cataataatc atcaacatct atttgattaa
    26821 cttttccagt atttcttttc attacacttt ctagaacaac tttctgattt tgttcctgac
    26881 caagtaaatc aacacccttt ttcacagcga cacctatagt agcaccattt gtcaaatcag
    26941 atatcttccc ttttatcgaa ccaagtattc cacttgccat atcctttaca gctattgcac
    27001 cactccatac cttacctgca aataatctca atttgggaaa tactttactt ataatgccac
    27061 ttgccatatc ttttatagct attacaccac tccaaaaagt atttttaaac aacattaact
    27121 taggcaaaat tctacccata gcactactca ccatatcttt aagattaata cttccttgat
    27181 aaggacgttt ctctacttgt tttaattttc ccaagatact acttataact ctcttagtca
    27241 tatcttttgc ttttattacg atattccaaa ccttgccacc aatctttttt aaatttccaa
    27301 gtaatttatt tttagttttt tctacttcat cacttttagc tttaaatatt aatataatca
    27361 taatatttga attaaactca tttttctttt gaatcaactt attaaaataa tcaataatca
    27421 tagctatagt tattgctagt ctcataatac ttccatttaa acttccaata actcctgaaa
    27481 cagcatttat gctagaactt aattttgtca tagttgaact tacactacta gcagtagttt
    27541 gaacttttct taacgaggaa tttaaactgt ttactctatt acttgcactt ccaatagaat
    27601 tttttatgct actagccatt acattagtgg aaagtgaaac agccacagtt gctctcctaa
    27661 aattatcagt tactgatata atttgattca tttttggtgt gtaattatct gtaaggtaca
    27721 tatttatacc ttcttctctc acatttccca ttacatacca cctcctgtca aaggacacac
    27781 tccatatcca tgtttcattt tttcaacttc ttcctcaatt tctaaggcga aaaaagcttc
    27841 aataagcttt aattcgcctt tatccattgc ataaaaaagg gacggtctta tacctttttt
    27901 cttccaataa tagtacatta ttgtagtcct ggtatccgtc cctattagtt ttttacctct
    27961 tctatcactg catttttacc ataacccata acatcttgaa gtattctata taagttggta
    28021 atttcacctg gtaataataa ttttcttgct aattcttttg gtgttggagc cttaaattta
    28081 tccattagct ccttattttt aaataaaagc tttccatcca aatcacaaac accttctata
    28141 attgtaagca tctgcatctc cggtaagtct atatccgttt tcatattttc atcaatttta
    28201 agacaagtat tttgtatttc ttcatcttta cttattgtta aagctctaca cattacttta
    28261 aattcctgcc caaatatagt tgataatctc ttaagttcaa ctatagtact cggtctttct
    28321 aagttttctg tatctgaatt taaaagtaaa tctactatgt ttatattttt tgataatcca
    28381 ttttcactca ttattatttg ctcctttatc aatcaaattc aatcaactaa cttttttact
    28441 tattaactat ctgaatttat taagtatata tttactaaat atcagtataa aaccaaatat
    28501 aaaactaaat ataaaactaa attatatcaa gaaaatcata ctcagtaaat gtgaaaggtg
    28561 cttctatctc tccttttacg ccaacctccc aatcagctaa agttaaatca tcaaatgcta
    28621 tattctttac tgctattctt tctgctccat aagaatctgg gtcatttaat ttagatatag
    28681 caacaaacct aggttcagaa cctctcttta tcttttctcc aattaacttg tgcattcttg
    28741 aactaacgtg gtatagagtt attgagcctt ttcctttata tcccatatac tttgtatcag
    28801 tacccatttg acctgctatt ataatatctt cttttgtaaa ttccatcttt gcttgaaatt
    28861 tctttacttc tgctactttg tttccatcaa gccaaagttc tccccaagta ccactcatta
    28921 catttctagc ttccatattt gccatattaa taatcctcct atatttctat tgataaatct
    28981 atatcttcca tagcatcaag tacttttatt tttgctttta aaaatacttt agaacctgtg
    29041 ttagcttctt ttatttcttg taatgtcata taagataaat ctactccttt tgattttaaa
    29101 tacgattttt gtgcttcaaa atctatttca acagtagaat cagattctat aagtgctgat
    29161 ttttctaatt cttctaaata actttttata gctactatca ataaacattt gttgtcataa
    29221 ctgttagtaa cctttcctat atagtcatct attatcacct ttcttatgtc actatgtata
    29281 atatctaaag tgtcaactat ttttattttc tggaacattt ctcctttttc tgctgttaat
    29341 tcagttaaag aatttactcc tctagcaatt cttatagctc ctgcttcttt aataagtata
    29401 agctctcctt tattaacctt tgattctgca tcaactttcg tcatcttagg tatatcgact
    29461 acatcactaa gtttagtata agttactgat tgacttaaag gtgtacctgc tataagtcca
    29521 gccaccctac ttgtaaactc atcaacactg tatttctttt ctccaactaa cacatcttct
    29581 gtagtaaaat taattatccc ttcatgattt cctacaactt ttcctaatac agctttaacc
    29641 ttaaccttat ctatatctct aagtttaatt atccaatttt ttatagcagt cttatcagct
    29701 tctactgctt ttggcataca tagataatta aattccttag tctctaaaaa atctaatgca
    29761 gtttgaatat ctgcttctcc ttctattaca taaactaata acttatttgg agtgttaaca
    29821 tttcccatca aagctaaatt tatatattct ttattttcag cagataaatc aactggtata
    29881 tcctcttttt catgtatttc attaagacct agtgccttag catctttaag taccattgca
    29941 attattcctc tagctgaacg ttctttaaca gttgtagcta gctccttaaa tgatatgttg
    30001 atacttggta atcctatagc catactatca ctctcctaaa tttaaattga tttcttccat
    30061 attttcatat actacatctt cttcatatac actatccaga tagtttagtg taaatgtaaa
    30121 gttcaataca taaattgact cttctttttt aatactttgt tctagctttg ttatatttaa
    30181 ttttctgtct gttaccttta tatttctgtt gaataactta tttaattcat ctgaaacttc
    30241 atacattcta atactctttg actgtggttt tggtaagtat tttatagaga taacaatatc
    30301 tttgttattc gtatttaaca ttacaggtgt acaaataact gatagtatac ttacaaaaaa
    30361 acaagaccct tcagagcctt gcacactttc atcatcaata aaaatacttt cagtaaagtt
    30421 ttttttgaga atctcaataa ttgtttctaa tatttccttg tatttaagca aataatcacc
    30481 acactatcac taaatattct tgcttaatct gataatctta ctataaatga tataagtcat
    30541 ttttaaatat atatctcatt tattcctgtc aagtatcttc tatattttta ggtaatatta
    30601 ttcttttcaa tctatcacct ccttcaatat ggatttcttt cacaataaca ttatctcaca
    30661 tattttattg cctattgttg taatggtgta tataaagtgt tgtaaaattg tcctattttt
    30721 tataatatct cactcaattt aaatatcatc tttttcttta aattgtagca agatgttcta
    30781 tctaagtgta gtttcataga aatatatgtc atattgtttt ttgtcttact attataaaaa
    30841 agattaaaaa attccatttc tatatcattt agacacgtta gagcattttc tatcttctct
    30901 ttttctattt ctatatcttc tttttccatt tttaattttc ttaatctttc ctctcttttt
    30961 aacacctcat tttccacaga agagcttatg ttatatgtgt tactagtctt ttctgtatat
    31021 accattgctc cacatccaga gtattcattc tcacatcttt ttatgtccaa gttaatattc
    31081 tttatcttta tatccaaata cttataatta tgtaatcttt tatctgtttc tttaaataat
    31141 atatcttttt tgttttccat agtacttccc cttttttata tacttattat tagcttcaaa
    31201 tttacaataa tctctttagt gcacagtttt ttactaatct cacaaccttc aagaacatat
    31261 tgttctataa ctgtataaaa aaaacttctt ctatattttg ttctaaaata ctagaaatct
    31321 tcttagccat aagaagtcca ggatttttta tgcctctttc tatttgagat atataatcat
    31381 ttgtaattcc agcctttctg gcaagttgaa tttgtgtcat acctttgcat tttctataat
    31441 atttcaagtt atttttaaac attgttttat acctccatta ctattaagaa tattttgttc
    31501 ttcctcactt agattatata gaattatttg ttctttatca atagatttta gaaaaaaata
    31561 ttcttttata ttttattagt tttatttttt agaacaattt gttataatct atacataagg
    31621 tggtgttata atgtttgcca aaagattaag agaacttaga aaggaatttg gattgactca
    31681 aagagaactt ggagaaaaag taggcgtttc ccaaagagtc ttagggtact atgaaacaga
    31741 aaacagattt cctgatgaac acattttaaa taaattggct gatgtattta atgtatcagt
    31801 tgattacctt cttggaagaa ccttagttaa ggaaaatatt gatacagtag ctgcacatag
    31861 aaaaaatcca catgaagagt tacctgaaga agcacaagaa caacttaatg attatataga
    31921 atttttacta aataaatata aaaaaaaata aaccatatga tgagcagtct atttttctgc
    31981 tcttttaata taattcaaag gcaaacatac attctatttt agggggattt ttatgaataa
    32041 tttggataag ctttttgaat tagcttctca agaagagata ataattcatt ataccactta
    32101 tattgcaggt gatttagaag gactatatat aaacaaacat ggtattaaaa ttatatcatt
    32161 actcagtaat ttaaaacaaa actcaaaaaa attgacatcc atcttagctg aagaacttgg
    32221 acatcatttc actagccttg gatactatgt atcctcttat aacgattatt atacaaaaat
    32281 tattatagat aagtgcgaaa ataaagcctt aaaatgggct tgtgagttct taattactga
    32341 agaagatata ataaacataa ttaactctgg catcacttgc gtttatgaaa tggcagacat
    32401 acttaatgtg gatattactt tctttcaaaa aagattagaa tttctatcat taaaaaaaca
    32461 gtctttacaa cttggaaata ataaatattt aatattaact aatttgccgt atttctacat
    32521 atttgatcca atttcataat aaaaatagta gtaattttaa ctggcttaac agtaaaatct
    32581 atatatttat tttaacaaat tttggtttct ataaattcaa tattaatata
//
LOCUS       DAGSHT010000023        31725 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGSHT010000023 DAGSHT010000000
VERSION     DAGSHT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.024"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1219
                     /locus_tag="MJB72_001012"
     CDS             <1..1219
                     /locus_tag="MJB72_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBY8262137.1"
                     /translation="RNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDY
                     ARIVEIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLG
                     IDRVKNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLA
                     LFNSKDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIP
                     GDGLVCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYD
                     YGCFVIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFD
                     TIKALNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYL
                     INLDIFMREEDKN"
     gene            1237..1836
                     /locus_tag="MJB72_001013"
     CDS             1237..1836
                     /locus_tag="MJB72_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBY8262138.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(2063..4192)
                     /locus_tag="MJB72_001014"
     CDS             complement(2063..4192)
                     /locus_tag="MJB72_001014"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBY8262139.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLARPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            4458..4706
                     /locus_tag="MJB72_001015"
     CDS             4458..4706
                     /locus_tag="MJB72_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262140.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            4872..6263
                     /locus_tag="MJB72_001016"
     CDS             4872..6263
                     /locus_tag="MJB72_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBY8262141.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            6378..7847
                     /locus_tag="MJB72_001017"
     CDS             6378..7847
                     /locus_tag="MJB72_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBY8262142.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            7912..8505
                     /locus_tag="MJB72_001018"
     CDS             7912..8505
                     /locus_tag="MJB72_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBY8262143.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            8687..10033
                     /locus_tag="MJB72_001019"
     CDS             8687..10033
                     /locus_tag="MJB72_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY8262144.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            10215..10586
                     /locus_tag="MJB72_001020"
     CDS             10215..10586
                     /locus_tag="MJB72_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBY8262145.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            10579..11268
                     /locus_tag="MJB72_001021"
     CDS             10579..11268
                     /locus_tag="MJB72_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBY8262146.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            11788..12654
                     /locus_tag="MJB72_001022"
     CDS             11788..12654
                     /locus_tag="MJB72_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY8262147.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            12896..13705
                     /locus_tag="MJB72_001023"
     CDS             12896..13705
                     /locus_tag="MJB72_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262148.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            13849..14913
                     /locus_tag="MJB72_001024"
     CDS             13849..14913
                     /locus_tag="MJB72_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBY8262149.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            14941..15660
                     /locus_tag="MJB72_001025"
     CDS             14941..15660
                     /locus_tag="MJB72_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY8262150.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     IFIPKMIEKGFKFVTVSELIK"
     gene            15684..16268
                     /locus_tag="MJB72_001026"
     CDS             15684..16268
                     /locus_tag="MJB72_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBY8262151.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            16270..17619
                     /gene="pabB"
                     /locus_tag="MJB72_001027"
     CDS             16270..17619
                     /gene="pabB"
                     /locus_tag="MJB72_001027"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBY8262152.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTSIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCANKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDVTDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            17609..18352
                     /locus_tag="MJB72_001028"
     CDS             17609..18352
                     /locus_tag="MJB72_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBY8262153.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIIRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            18386..19087
                     /locus_tag="MJB72_001029"
     CDS             18386..19087
                     /locus_tag="MJB72_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262154.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            19113..19685
                     /gene="folE"
                     /locus_tag="MJB72_001030"
     CDS             19113..19685
                     /gene="folE"
                     /locus_tag="MJB72_001030"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBY8262155.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            19749..20540
                     /gene="folP"
                     /locus_tag="MJB72_001031"
     CDS             19749..20540
                     /gene="folP"
                     /locus_tag="MJB72_001031"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBY8262156.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSYGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            20551..20916
                     /gene="folB"
                     /locus_tag="MJB72_001032"
     CDS             20551..20916
                     /gene="folB"
                     /locus_tag="MJB72_001032"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBY8262157.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRARDE"
     gene            20909..21415
                     /gene="folK"
                     /locus_tag="MJB72_001033"
     CDS             20909..21415
                     /gene="folK"
                     /locus_tag="MJB72_001033"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBY8262158.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            21396..22181
                     /gene="aroF"
                     /locus_tag="MJB72_001034"
     CDS             21396..22181
                     /gene="aroF"
                     /locus_tag="MJB72_001034"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBY8262159.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            22436..24226
                     /locus_tag="MJB72_001035"
     CDS             22436..24226
                     /locus_tag="MJB72_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBY8262160.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            24244..25410
                     /gene="rpoD"
                     /locus_tag="MJB72_001036"
     CDS             24244..25410
                     /gene="rpoD"
                     /locus_tag="MJB72_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBY8262161.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            25575..26264
                     /locus_tag="MJB72_001037"
     CDS             25575..26264
                     /locus_tag="MJB72_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY8262162.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            26278..27375
                     /locus_tag="MJB72_001038"
     CDS             26278..27375
                     /locus_tag="MJB72_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBY8262163.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            27438..28010
                     /locus_tag="MJB72_001039"
     CDS             27438..28010
                     /locus_tag="MJB72_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY8262164.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(28070..28537)
                     /locus_tag="MJB72_001040"
     CDS             complement(28070..28537)
                     /locus_tag="MJB72_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY8262165.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGIAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDYISGKASRL"
     gene            complement(28922..29398)
                     /locus_tag="MJB72_001041"
     CDS             complement(28922..29398)
                     /locus_tag="MJB72_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBY8262166.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(29518..30609)
                     /locus_tag="MJB72_001042"
     CDS             complement(29518..30609)
                     /locus_tag="MJB72_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBY8262167.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKKFKKLLSLGFIIDFILVFILVFTFNKLELSYFVGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTFGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMDITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPFGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(30602..30991)
                     /locus_tag="MJB72_001043"
     CDS             complement(30602..30991)
                     /locus_tag="MJB72_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262168.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSNLFDKYEVKYYE"
     gene            31183..31629
                     /locus_tag="MJB72_001044"
     CDS             31183..31629
                     /locus_tag="MJB72_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262169.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
BASE COUNT        12915 a         3260 c         5504 g        10046 t
ORIGIN      
        1 taggaatatt gatgatgata gtatagataa agaagcagat atatataaat ttggaaaggc
       61 aaagacaaga gatttagagg atataaagat tatatttgaa atctttttag agtatgatga
      121 aaaggatgat tatgcaagga tagttgaaat tgataagaat tataatgact ttgaatttcg
      181 agtagtacca ttaaaaatag cagatttatt tgaagaaaat attttatctc aattagaaaa
      241 aggaatattt ttatcagcaa ctttgagttt atctgaaagt atgtcttatt ttaaaaatac
      301 attaggaata gacagagtta agaatgtgga aaagattata gaaccaattt ttgattataa
      361 aaatagagtc tctgtagttg gattttctga tatttgtgag tacagaaatt ctgaatttcc
      421 aaatgagatg agtaaaatta tatctaatat atcaaagatt acagaaggtc atacattagc
      481 tctttttaat agtaaagata gacaagaaaa aacttatgaa atattgaaaa aatacttaca
      541 tagttttaac ttagaaattt atgctgataa aaagggcatt agacacctta acgatttaaa
      601 tagaaaatgt gtagtacttg gctcaaaagg atgttttgaa ggcgtagaca ttccagggga
      661 tggtttggtt tgtgtaactt tagacaagct tccgaatcta aatcctaaag accctttgta
      721 ttttactata atgaagaagt atggtataga ctactatact ataaattatc ctcaaatgac
      781 cataaaggta aagcaagcta tggggagaat tttaagaagc aaatatgact atggatgttt
      841 tgtgattttt gatgtaggaa ctaatataag tgtgttaaag agattagaga aagatttaca
      901 cgattgtaaa atttcaaagg ttaatagcaa tgagttttat acttatataa ggcgacattt
      961 aaataaatct agaagtttaa tactaaaatc tgttatattt gatactataa aagcgttgaa
     1021 tgttgatgct aaaatggata acaatgatgt tgataaggat attataaaaa aagatataaa
     1081 tgaaaatata agacaaagag ctgtaaaagg agaagtgtac catattgata ttataaaaaa
     1141 agatatgaaa gtaaaatatt ttgatagaaa ctatcttata aatttagata tatttatgag
     1201 agaagaagat aaaaattaag agtgggagtg atatatttgg caaaacaaaa gttttatgct
     1261 gtgaaaaaag gtaaaaatat aggtgtatat aatacatggg atgagtgcaa aaaacaagta
     1321 aatggattct caggagctga atataagagt ttttctactt ttcaagaagc taaagaatat
     1381 attgatgggt cagaaaaatt gagttttcaa gaagataagg agtttataga agcatatgta
     1441 gatggaagtt atgaacattc agtaaaaatg tatggttcgg gtgttgtgat tttaaaaaat
     1501 aatgaagtta taaaaactta tagtgaaaaa ggaaaagaga aaactttagt aagtatgaga
     1561 aatgtagctg gagagataga ggcttctaaa attgcaatgc agtactgtat tgacaataat
     1621 gttcaaaact tgattttata ctttgactat gaagggattg aaaagtggtg tactggagtt
     1681 tggaaaacaa ataaagaagg aactatagca tataaaaatt tctatgacag tattaagaat
     1741 aagcttaatg taaaatttac aaaggttaaa gctcattctg gaaataagta taatgaggaa
     1801 gctgataaat tagctaaaaa agctattggt gtataataaa aaaatattta tacataatta
     1861 catggtataa gagtatatct aaattcctac tttataatta aacaacttgt aattaaataa
     1921 attttgatta aaaaagcaga ttgtattatg aaaaaataag tatctaattt ataaacaatt
     1981 aataatagta ttaattagat acaacttatt taatataata aaatctgctt tttatataac
     2041 tataaaattt tactacattc tattagaatt gcccataaat accttcaagt tcttctttta
     2101 atacttcagg gtcatttata ccaatagaca tggttctagc cagtatattt gcttggtcat
     2161 aagttgatgt ccatgagaat acaccaccta gacaatttct aagaacatat tgagttttta
     2221 agaatattga taaagcatca tcatatgaca ttgcaaaatc tccatcctta actaaatacg
     2281 gaacttgagc agtattatca aatctatatt catatccatt tttatttata taatcccttc
     2341 taagctcatc ataagttctt gttatagtag cacctaatct tccataaaat ggtataccaa
     2401 gaaggatttt ttcagaaggc attccagcat tctcaagatt tctcaccatt gcatcaacac
     2461 tatatcctgg caaagataag tctgaatcaa aaagatttgc ttgatgtttt ctaccatttg
     2521 ggcctgtttc acctgctgta aaatcataac tcataagatt aaaataatct attataggag
     2581 ctattttatc tatttcagca cttgaattta tatatcctct atctcctgtt ccagctacac
     2641 taagccattt atcatctcct ataacatctc ttatggcagt tagtaagagt gtaaaatttt
     2701 ctctatcttg aggtcttgat gttattccag atgcactact tccaggatat tcccagtcta
     2761 tatctattcc atctaaagca tattcattta tcatctgatt gacctgtctt gcaaaattat
     2821 atctggatgt aggtgttaat gctgcatcag agaatccttc agctccccaa cctccaatag
     2881 ctacaattac ttttaagcta ggtttttcac ctttaagagc aagtaattga tttaaaaatc
     2941 taggataagg aataaacaaa tttccagatt ggtctatctc agcaaaagca aatataactg
     3001 catctaatag ttgagcatct acattagttg gatttccaag aacatattct gctacaattg
     3061 gatttatatc tggacagttt gcattttcaa ctattggatt gtatgggtct gttatttgtg
     3121 gtctagctag tttttttgaa tcttttaaag tcttcatagc tattttttta gtattttttt
     3181 cactagtata atcgtagttt ttataattat gtttatagaa atcttcataa ctactatctt
     3241 ctttgttata gctagaattt attttgtcac aagattcatc tttatcatag ttaaaattca
     3301 tttcatcaca agacttatct ttaaacttat cttgtttgga gttgtgaact ttgttagtat
     3361 ttttagtatg ttttttctca tggttgcaat catctttaat atcagtcttg tgttcttgtt
     3421 tacagtcaag agctttgttc aggtagtcat gatgttcatg ttcacagtta cattcatttt
     3481 caatgttttt agtatgttct tttttccagc tacaagtatt atcaattttc ttgctcactt
     3541 cttcatcatt ataattatct ggtacaaaac ataagtacca gtccatacat gaatatttct
     3601 tattacaact gttaactcta ttttttaatt ccttatagtt tttaggataa ggtaaaatca
     3661 ctggcattcc aggaaaccag tttgcaggag taactatatt atctctatca ctagtctgaa
     3721 gtgcatctac tattctaagt atttctggaa tatttcttcc tgtagttagt ggataataaa
     3781 gaatcgttct tagaatttga ttattatcta taataaatac agagcgaaca gttgatgtat
     3841 cactcattgg ttttgatatc atgccgtata acttggcaat tctcatatct ctatcttcta
     3901 taataggaaa tggaatttct acacctgtaa gtaaagaaat attatacatc caagctaaat
     3961 gtgaactgtt actatcaaca cttagaccaa ttagttctgt atttcttttt ttaaattcgt
     4021 cataatattt agcaaaacat aaaaattctg tagtacaaac tggtgtaaaa tcaccaggat
     4081 gtgaaaataa aacaatccaa ttacccttat agtcagagag tctaatagga ccatttgttg
     4141 tattggcttt aaaatcagga gcctttgacc ctaaacttgg caaatttggc atgtaaatca
     4201 cccctcaata aattatcatt tattttattc tattaattaa tacatattag tgttactatt
     4261 ttattaaaag ttaagtaatc cttatgtcaa aaaaagatat ttattaaaaa gatttaatat
     4321 tttcctaata taaatgacaa tagtaattgc tattttttta ctaatttggt atagtaatgg
     4381 ataaggggta gtgtcaattt tagtgattat acgaggtgag tggattgaaa aaaattctaa
     4441 gaattttagt tatctccatg atgatatgtt ttgcaatgac ttttgcatta gtgttaataa
     4501 attcagttat gggagtacat ataggattgg attattactt tttgatggga agtgtgctta
     4561 cagtttgttt aacaattgga attgttttat gcaatgctag aaaaatattt gatagcaaag
     4621 ctatacacaa gacaagaacg aaaactgtca gtaataataa aaaacaaaag agtcattcta
     4681 gggatataaa aagaaagata tcataattta tgtaaaggaa atatttatgt ataaaataat
     4741 gattgatagg taaaataaaa acacgattta acttgtgttt ttattttttt tcaagaatta
     4801 tgatataata accaataaga aatttagttt accataaaat ataaagatta ttaaatggag
     4861 agggaactat aatgaatttt agagatttat ttgagaaggc tgtagatttt attgatgaaa
     4921 aaaggaaatc tatagttgcg atttcattat caacattagg tgtaattgct ttgataatag
     4981 tatttttctt aagtagtaac gagtttagtg taggaaatga agctaatgag ttgttaaaga
     5041 ttatagagaa aagacagtat tctatagctg ttgattacta cacaagtata gaaaaaaagt
     5101 tttctgattc aaaaatggaa aggtttaata agtctgtttc taaaaaaata aataaattgc
     5161 tattaaatag tggtgataaa tatttagatg gagacataag tcaagaaagt tttattggat
     5221 taataaatac cgtgaaagaa ttaaacaaaa taagtataga tgtggatgac ttattagctc
     5281 aagctgatag agttagagaa atgtataaag aagaaaatac tacttatgat attgccatta
     5341 actacataag tactgtttca atattaaatg gtatgggtag taacctagat gtatacaaac
     5401 agaatataga aacaatcaaa gagtctagag atgtttatga ctcagcagta aaagaccaaa
     5461 aagcatataa gtattatgaa gctattgaaa agtataataa agtattaaaa gaagatgaaa
     5521 agtattacag tatggctcaa aatgggaaag aacaatgtat tgaagaaatg tatgattact
     5581 atataagtag agctgaagag gcaaatactt ctggagatta tgaaagagca ttacaatata
     5641 ttgagtattt aaaagacgat tattcagatg atgaaaaggt acagagttta gaaaaaaaat
     5701 ataaaaagaa tttatcattg tatactttaa catctgatga cattataaat gtaatagcta
     5761 aaaaaagtgg caaagacaag gcaaatctat ctataaattc acttccacaa atgataaaaa
     5821 ataataaata ctattatgct gaagtatatg agtatgataa attaataaat gaagttttgg
     5881 taagtgcaaa aactaaagat ttatattcat ataaagatgg taaaaaagat tacaaagtag
     5941 attatggtga tggatacttt agaatattag aagatggaag ttatcaattt ggtattacaa
     6001 aagataaagc taagtttgtg ttaactaata ctcttgatga aaaagaaaat aagtataaaa
     6061 agattgagat attagatata gagaaagcag atagatatgt aaaaagtaaa aagagtttag
     6121 aagaattgtt tggaaaatat aaaaatattt attattatgc tgtagtaaat aaaggcttat
     6181 ttagaggcaa agaagtttat gctattaata tttacaatga aaaaatatat gccatatcag
     6241 aaaatggact aaatgaatat taattaataa tattaaatta atatataatt aaaaagtttt
     6301 tggggtttta aggcgcatta tgtagtaata taataatagg gatatataaa taattttatt
     6361 gtaaggagaa gtatgggatg agtaatgtga acaaaaagct agttatagct attggagctg
     6421 tagcattagt agtaataatt tttataggca taatgtgcat gcaatttaaa ggggagaaaa
     6481 tagcaaaaaa cacatatgtt aatggagtaa atattggaaa attaactaaa tcgcaagcaa
     6541 aacaggaatt agctaaaaaa tacaaattag agaatgttga ttttaattac aatgataaaa
     6601 gttggaaagt aaagagcaaa gatttaaatt tatctactga cctggataaa acagtagaaa
     6661 acgcatataa tttaaataga aagagtgctt tctttggcaa tctatcaaaa actatttcag
     6721 caaattttgg caaaaaaagc aacttagtag ttgcgataaa ttatgataaa aataaattaa
     6781 aagcagaaat ggaaaagata gctaaagaaa tagatgtaga cgtaaaagat gctacacttg
     6841 atataagtgg tgaaaaagtc aaggttatac cagattctga tggtcttaaa atggatattt
     6901 ctaagagtat ggagaatttt gataatcaaa ctaaaaaagg taactataaa aatgagttag
     6961 tagtaaaagc tactccagca aaagtaaaaa aagagcaact tgctaatata gatacaaatt
     7021 taggtacgta ttcaacaaca tttaaaactt ctcaaataaa tagaagtata aacataaaat
     7081 tagcaacaga taatataagt aatgtattac ttatgccagg agaaactttc tcatttaata
     7141 aacatacagg gaaaagaagt aaggaaaatg gatataaaag tgcacctgta ataatggaag
     7201 gtgagatgga agaagattat ggtggaggag tttgtcaagt ttcatctaca ttatacaatt
     7261 ctgtgttata cgcaggttta gaaattgtta atgtaaaaaa tcatactata ccatcaagtt
     7321 atgttccaaa aggaagagat gcaactgttg cagatagtgg aatagatttc ttatttaaaa
     7381 ataatctaaa gcatccagtt tacataaaaa attacgtttc aggaaatcaa attgtttgca
     7441 atatatatgg aagtgcagaa gataaacaaa atataacaat atcaacaaaa ttagatggcg
     7501 tttcacaaac tacaatgaaa agagttaatg accctacaat gcctaaaggt aaagaaaaag
     7561 tagacaaaag tggaagaaat gcatattctg tatcaacata tagaacattt aatgatgcaa
     7621 atggtaaaaa aattaaaact gaaaaaatag ctaattcata ttatcctaag aaggaaggta
     7681 ttatattagt tgggactatg gaaccaaaac cagaggaaaa accaaataca gacgaaaata
     7741 agaataatca aaatacaaac aatcaaaatc caaataatca acaaaaacca gaaacaccac
     7801 caactgataa taagcctaat gagacacaac cacaaccgca agcttaaagt tctcaagaat
     7861 aggatttatg tagagtaata aaaaatataa atctaaatag gagggattac tttgaattta
     7921 aataatataa cgaattacgt tcaaagtatt tgcgagaata tttctaatgt cttggatgta
     7981 gatgttactt tagttacaaa agatttaact aggatagcag gtacaggaat attcagaggt
     8041 aaaataggag agacaatatc agagaagtca gtgtactctc ttgttttaaa agatggaaaa
     8101 tcctatgtta taaataaaga aatagaagag aactgtaata aatgtgcaaa tagagaaaat
     8161 tgtaaagagt tagctgatat atgcactccc gttaaaatgg gagagcatat acttggaatc
     8221 ttgggtatag ctgcgtttaa tgaagaacaa aaagaaaaga tactttcaaa agataaggag
     8281 ttaactgctt ttgtaaggag tatgtctgat ttaatatctt ttaaactaga tgaactttat
     8341 aaacaagaag ttaagactat agatgacctt gagagggaag ctatagaaaa tgccataaag
     8401 aaatatggaa gtaatacaga aggtatgaaa aaagtagctg aggcattaaa tataggtata
     8461 gctacactct atagaaaagt aaaaaaattc aacattaaaa gttaatagta ttgaaaaaga
     8521 ggtaaccttt ttatgaaaag aagaggatta ccttttttat tatgagaatg gtggaaaaat
     8581 atgtatagtt ttgacttgac atattaaata tgttatctta aatatatatt ttagagtgga
     8641 ggtgagtatt attttagtta aagatataaa acaagacata gaaagcatgc ctagaaaaat
     8701 tttagatata tctttgatag aagataataa aagtgatttg acttttttta atatagaaga
     8761 agaagacggt gatgaaacat taaaagcatt aaaacagagt tatgtgacta caaattataa
     8821 agaagaaact ctattaaact tgcaaggagc tttaaattca aagtcagata caacgtctaa
     8881 ggagttaaaa ttagaatcat ataaaagata taacaatgca ttggatttag catataaaaa
     8941 ttatataact agtgccatta atatggttaa taaagctctg gagataaatc ctaaagacat
     9001 tgatatacta aatcttagag gtcttttaaa attattaaaa tgtgattttg ctaaatcatt
     9061 tgagagtttt tatactgctt tatgttatga aaataatcat atatctagaa aatatgtaaa
     9121 tctaatttca tctgaagatt ttaaaacttt tttaggaaga tacaatcatt ctataagatt
     9181 tataaatgaa gaattaaatt atgagtcaat acatatttta gagaacataa ttgaagaaga
     9241 acctgaacta atagagccat atgtaatttt atctttgtta tatgataaac taggtaatgt
     9301 aaaaaagaga gaaggatatt tagacaaact taaagagcta gataaagata atcctgtatt
     9361 tgaaaaaaat gaagaagaaa aagaagatac aagtaaaaag gaagaaataa aaaaagtaaa
     9421 aagaaagaaa agtattttgc catatattat agttggctgt gttttaatag gtatggggat
     9481 atatttgatt caaagcaaaa agagaataga aaacttgaat aaccaaataa gtagtaaaga
     9541 ggaaaagtta agtgaaactg ataaaaagtt aggagaaacg agtaaaaaat tagaaaaaac
     9601 gaataaggaa ttaaatgagg ttaaaaataa agaaccagaa agagagataa ctgtagctga
     9661 tgaggaagat ttatattatc aagctctaaa tttaaagaaa aacaaagaat atgaaaaagc
     9721 catagataat tttaaaagtg tagtttcttc tggtaaaact aaaaaatata tttctgagtc
     9781 aatatatcaa ttagcaataa caaataaatt attaggaaat aaggatgaag ctataaaata
     9841 ttacaaaaaa tatataaata cttatactaa aaatgaccag tattatgatg attcatatta
     9901 cgagttgggt atgctttatt atgataatgg cgatttaaaa aatgcacaac agacttttta
     9961 tagtttgaga agtgaagttc cagacagtat gtataacaat tcaaaaataa aagaaatttt
    10021 aagtgaaaaa taggtttata tagttaagtt ttttgaaaca accaagtatg tattttatta
    10081 aattaaaata aaaataaatt taaaaagaga ttgtgtcaaa ttataacttt ctattttaaa
    10141 gaaagatatt tgcttgttga taaaactaca aatatatgat ttgaagcaat ctctttaatt
    10201 tttggaggtg gaaaatgaaa gttttcaatc aattagtaat aattttatct atctgggtaa
    10261 taggagagta tataagttcc tttatacaag gaattatact tattcctggg agtatagttg
    10321 gtatgctatt attatttcta ctactacaat ttaaagtttt agacttgagt agtatagaaa
    10381 atgtaagtgg atttttcctc gataatatgg ctatattttt tattccagca ggtgtatcac
    10441 ttataaagtc attagaccta ataagggaaa atgtattagt gcttttatta gtcatatgtt
    10501 taagtacact aatagtaatg tatactactg gaattattgt ggagaaaatg ataaaaaaga
    10561 aggaagaagg acaaaaaaat gtataatgta ttacagacac cagtatttgg tataatagta
    10621 acaatagttt ttttcaattt aggaagatac attcaacgaa aaacaagtaa tcctatatgt
    10681 aaccctttat taattgcaat cgtaggaata atattgtttt taagcatatc taaaatccct
    10741 tatgagaatt acaagatagg agctgatagt ctaaatttct ttttaggtcc tgtcacagta
    10801 gtacttgcag ttccacttta taaacaattt gaattattta aaaagcatat gtttgagatt
    10861 attgtaggaa taggttgtgg tatagtgatt tcttttgtat ctgtattgat aattggcaaa
    10921 atagctaatt cagatgtaag tataataaat tcactaattc caaaatctat aacaacacca
    10981 atgggaatat ctttagcaaa ttctttaaat ggtatagaat caataacagt tgtagccata
    11041 atttttacag gtatttttgg tggtatggtt gcttctacag tattcaaact ggggaatata
    11101 aatcatccag ttgctaaagg aattgctctt ggaacatctg cacatgcttt aggaactaca
    11161 aaggcttttg aactgggaga aatagaagga gctatgagtg gtgtttctat tggagtctct
    11221 ggtacaatta cagtaatact aattccaatt ataatgaatt ttatataaaa aataaaatta
    11281 agacgagtac cttgaatact taaaacagaa taaaataaaa aaaatttaaa ataaaaactg
    11341 aatgaaaaaa atgcaaataa tgaaataaaa aaatcatgta ctgaaattat aaaaagaaaa
    11401 atttcattta ggaattaatt ataaagagac aattactaaa cttgaatttg ctaaaataaa
    11461 ataaattact gcgaaaatag atatttagaa aaaaatacta taaatttact atattgcaag
    11521 ttaaataata agcagtatca tattatgcta gaaaaagttt catttaaggc ataaatgaga
    11581 tgatttgaac gtttttaaag attagaatat aaaagattgc actagacata tatgaaaaag
    11641 ataatataat agatgatgta atagatgaca gttcaagaaa ttataaaaaa aatttatttg
    11701 aaatattatc tattacatca ggaataatat atgtaagagt aagcttttaa aattctaaag
    11761 atttagaaag attgaaggga gaaagaaatg agtgaagtta agaataccct acaaaaacat
    11821 ccagttttaa gatattcaac aatgctattt gggatgttga tgacatctat aggaataaat
    11881 gggttcttaa gaccagcaca tctgcttagt ggaggtgcta caggtatagc aacatctata
    11941 aactatttga caaatataaa tgtaggttta ttaacatttt taatcaatat tccaatattt
    12001 atcttagggt tcatatacct tgaaaaagag ttttgtattt caagtttggt aaatatgatt
    12061 gtattttcat tattattagg agcaactcag gaaataagta atattatacc tatacatgat
    12121 atattgcttc aaagtgtata tggtggtata ttatctggtt taggagtcgg agttgtattt
    12181 agaactaggt cttctcaagg tggtacagat ataatagctg caatactaaa aattaagaag
    12241 aatatagaaa tgaaagacac tgctttagca ataaatggct tgatagtact tacaggaagt
    12301 tttttatttg ggttggattt agcattatac actcttatag gtctatttct aaatgcgtat
    12361 tcaatgagtt ttattaaaga tgcaatgaat taccaaaaat ctgttatggt aatgtcaaat
    12421 gaagtcgatt taatagcaga agatataatg aagagtttag ttagaggtgt aactttttta
    12481 gatgcagaag gtgcatacac tcatcaaaaa aagaagatta tatatacaat agtttcatca
    12541 aatgagatac caaagataaa agatatagct ttgaagtatg ataaaaaggc ctttatatct
    12601 gtaaatgatg ttacagaagt aaagggaaga gggtttaaag caaaagattt atagaaaatt
    12661 ttacaagctg ggagtttata atgtaaattt gtatattata tctctcagct tatttaaatt
    12721 tagtatacga aaataaaggt tttattaatg atttgtgtat cactttaaaa gatatcattt
    12781 taaaattaaa attgaaaaaa ttacttaata tgtagaaaac atgaccacta ttaggatata
    12841 ataaatttaa tagatttagt aaaatatcta ggaataacgt tggaggaatg gcattatgaa
    12901 tttatctaaa gaatatgtta atttgtatga taattatttg aaatatataa atgaagtaaa
    12961 caaagagatt agaagtataa agaacatgaa gaaacgtgtt ttaaaaaggg gcgattttgc
    13021 tccagaaaat gaagtattag aattggaagg aaaaacaata cgcgatatag atgatgtcga
    13081 tacaaaaaaa ccaaaaaata aaatctataa attttcaaat ggggatgtat atgtagggaa
    13141 attcttagag ggaaagatgg aggggaaagg ctcatatact ttttttgtag atgaaggaac
    13201 agttatggag tacataggag aatttaaaga agataaaaaa aatggaaaag gaaactttat
    13261 attttcaaat ggaaatgaat atataggtta ttttgaggaa gatatgatga gcggtattgg
    13321 aaatatgtta tatagtagta aagatgaata tattggaact tggaaaaacg gaaaaaaaga
    13381 tggaaaaggt atttataagt ggagtgatgg atgtatatat gcaggtgaat ttaaaggtgg
    13441 aaagatggaa gggtacggta tttgctataa tagtaaaggg gaagtacttt atgaaggaga
    13501 atggaagaat aatttgattc atggtaaggg aacttatata tgggaaaaag gtaaaaagta
    13561 tataggtgaa tttatgcatg gtaaaaaaca tgggcaagga actttttatc taaataatga
    13621 attggtttat gaagggactt ggaaatttga taagccatct atatttgata gaacattaga
    13681 tgaaatattt tccttgagac tgtagcttta tctaattagt agataaagct tttttctaat
    13741 acttactgta aataggtatg aatgatattg attagtatac actatcaata ttaacttaat
    13801 aaatgcaata ttaaaaaatt ttgattgatt ttaagaagga gagatatatt gaagagatta
    13861 attgctttag tgttagtagt acttattgtt ttattttata gtcctgaatt attaaaagaa
    13921 ggatatgatt taaaagaaaa gttttttcaa agtgaaatat ggaatgaatt tttagacaaa
    13981 ataaatataa atgataattc taagaaagat aaaaaagaaa aaagtcaaaa tttacaatca
    14041 aataatactg aaggggagaa cttaggagag tcagatataa agaattttga aaaaataagc
    14101 ttaggagata atttaagcta tgttttatca agtattggca aacctggtag aattgatatt
    14161 agtgaatatg gttttgattg gtatgtatat aatcaatatg gaaaagaatt tgcaatggtt
    14221 ggacttgaaa atgatgaagt agtagcactt tatagtaatt ctattaattc atgtgaaaat
    14281 caagatataa aactaaatca agatagacaa acagtgagaa ctaagattac accattaaaa
    14341 tataagagaa aaggaaatac cagatatata ataaactcag aaaatcaata tgatataata
    14401 agtaaggaag gtaagtatat tacaattttc tacgatattc atgaagaaaa tagagtttgt
    14461 tcatatttaa taattgataa aagcactgaa gatgagttta aaaatttata tccagatgat
    14521 agcgaagaac ttaaaaaatg ttttgaactt gaagttatag accttgtaaa tagtgttaga
    14581 aatcaaagag ggttaaactc attgagatac agtgaacagg caactttgag ttcaagaaaa
    14641 catagtgaag atatgagaga taataatttt tttgaccatg taaataagaa gaatgaaact
    14701 ccatttgaca gaatgaaaag agaaggtata gtatatactt cagctggaga aaacatagca
    14761 gcaggacaga ttaatgcaat atatgcgcat gaagcttgga tgaactctga aggacataga
    14821 aaaaatattt tggggaacta taataatatt ggagttggag taatatttgg aggaagctat
    14881 aaaacatatt acactcaaaa tttttataaa taactaaaat atttgaaagg aagttaaaaa
    14941 atgacatcat ggaaaaaaaa gacagtatat aagtgcttaa tagcagtagt cttgttttgt
    15001 ggaatagtgt taatatctaa ttttagtaaa gtttctgctt tgatgatgga tactaatgga
    15061 aatgttttga ttaaacatgg ttcaagggaa aaaaagctaa tagcaattac ctttgatgat
    15121 ggtcctcatc ctaaagaaac tagtcaagtt ttagatgtat taaagaaata caatgtaaaa
    15181 gctacttttt ttatagctgg aaaacatgca aaatggtata aagaaccatt agtaagagca
    15241 agtaaagaag gtcacgagat aggaaatcat actttcaatc atcctgatat aagtaattta
    15301 agttctagtc agatagaaga ggagattgtt aagtgtgaag acattttgaa agaagtaact
    15361 ggaaaaaaac cgacattatt tagaccacca tttggaagtt atagagaaaa agaattaata
    15421 gaaattgcaa aaaagcatga ctataaagtt gttttatgga caggtgtaga tgtgaaagat
    15481 tggaaaaatc caggcgctaa tagcattgca gataaaataa ttaataaagt acaaaatgga
    15541 gatataatac ttttgcatga ttatgcaact aatgatacag ttgaagcttt agatatattt
    15601 attccaaaaa tgattgaaaa aggttttaaa tttgtaacag tttctgagtt gataaaataa
    15661 tttttagggg ggagatttac agtatgattc ttatgataga caattatgat tcttttgtat
    15721 acaacctagt ccaatatata gaagaattag gggaaacagt tgtagtaaaa agaaataatg
    15781 aaatcaaaat aagtgacatt gaagaactaa atccagaggt aattgtgtta tctccaggac
    15841 cgtgttctcc aaaagaagct ggtatttgca tagatattgt tgagcatttt aaggggaaaa
    15901 aacctatact aggaatttgt ttaggtcacc aaactattgg acatgttttt ggaggagata
    15961 ttataaaagc acaacagcct gtgcatggaa aagtatatag tatcaaccat actaataaag
    16021 gagtttttag gggacttaaa aatcctttaa atgtcactag gtatcattca ctaataattg
    16081 attccaatac agttccaaaa gaattagaaa ttacagcaat tactgataaa ggggaaataa
    16141 tggggattag gcataagaaa tatttaatag aaggagtaca gtttcatcca gaagctattt
    16201 tgtcagagta tggtcatgaa atgcttaaaa attttataac agaagctaga gaaagggtac
    16261 atatgtaata tgataagaga gataaatact aaattaaatt cttttgagat atttactatt
    16321 tttagaaatg aacatgacag ttttatatta gacagtgcta tggataaaga aaaattggga
    16381 agatattctt ttataagtag ccaaccattt aaggtattaa agtataaaga tactgatgaa
    16441 aatcctttag aagttttaaa agaagaacta cataagtata gggttgtaaa tgatactaat
    16501 cttccatttg taggaggtgc tgtgggatat ttatcttatg atttaggtaa ttacatagaa
    16561 aatttaccta gaactgctgt agatgatatt gagatgcctg atatgtattt tggattttat
    16621 aatcatgtaa tagttataga ccatcttgtg caaaaaactt atatagctac tccaaacata
    16681 gatatcgaat tagaagaaaa gataatagat gatatagaac aaagaatatt aaaagaagag
    16741 aaaaaaggta tagatagcat atgttatgaa gaaaaagaag taacatctat aagacttaaa
    16801 tctaatttta caaaggaaga atttaaaaat gcagttcaaa gtgttagaga gtacataaga
    16861 caaggagata tatatcaagc taatttaaca caaaggttca gtggagaaac agaacttaca
    16921 agctttgaat tgtataggga cttaagaaga tttagcccag caccatttgg ggcattctta
    16981 aactttgaag atgcacatat attatcaaat tctccagaaa gatttataag atgtgctaat
    17041 aagagaatag aaactagacc aataaaaggt actcgtccaa gagggaaaga taaagaagaa
    17101 gatttaagac ttcaacaaga acttagaaat agtgaaaaag atagagctga actacttatg
    17161 atagtagatt tagaaagaaa tgatattggg agaatatcta aaactggaag tgttaaagtt
    17221 ccagagctat ttgtaattga accatatgca aatgtaaatc acttagtttc cacagttgta
    17281 ggtgagttaa aagatgataa agatgttact gatgtaataa aagcaacttt tccagggggg
    17341 tctataacag gagcacctaa gattagagca atggaaatta tagacgaatt agagccaacg
    17401 caaagaaatg tatatactgg ttcaataggt tatataggtt ttaatggtga tatggatttt
    17461 aatatagcaa ttagaacaat aatcaaaaat gacaaaaaag tctactttca agttggagga
    17521 ggaatgactt gggattctga cccagatgaa gaatatcaag aaacacttga taaagctaaa
    17581 tccataatga aggctttgag gggatattat gaagaataat gtaggttttg atagtagttt
    17641 aagtaaattt ggaattggtt tgtttgaaac tattagagtt aaaaatggaa tagcagtaga
    17701 tttagatatc catatagaaa gaatgctaag ctcaataaat tgtttggatt tagatataaa
    17761 ctataaaaaa gatttcttga taaatgaaat tgttacatat ataaaaaaag aaaatgtcat
    17821 taataaagca cttagaatta ctgtttttga tgaaggttat aatatatcta ttagggatat
    17881 tccatataat caagagacgt atgagaaggg gtttaaatta gctatatctc caataattag
    17941 ggggaatagt ttaattcata gacataagac tactaattat tttgaaaaca tatataccaa
    18001 aaatatcgca aataagactg gttataatga tggtatattt ttaaattcag aaggtgtaat
    18061 attagaatgt tctatgagca atattttctt tataaaagga gaaagagtgt acactcctag
    18121 tgatagatta ccgatattaa atggaataat aaaaaaaagg attatagaga tttgtgacga
    18181 attacatata gagttgatag aaaatgaaat taatatatca gaaattagta gttttgattt
    18241 tgtttttgtc acaaatagtc ttatgggtgc tataaaggtt acagaaattg acaaaataaa
    18301 atttaataaa gaaaatgttg tttttgataa aatagtgaag ttattagaat aaattaaaat
    18361 attaacaatt tgttggagac aaaagatgat aggttgtatt tatgaagtat acaatgaaga
    18421 gatgactaaa ataatagaaa accaaaacac tttggctgta tatttagtgg gttctagtaa
    18481 agacgtagat tttactcttt atgatgtaga gataaacgat attgatgtgt tcgtttttgt
    18541 aaaagaaggg gaaaaacaag agagaatact gtttaaaaaa aagggaatag aatttgatgt
    18601 aaattacttt tcaaaagatg gtgtaaaaaa attattatca aatagggaat atttctttgt
    18661 gaaagaaatg aaagatgcta aagtactgtt tgatagggag aatatatcac atattataaa
    18721 agatataagt agagatattt acttagaagg acctagtaaa atgtcattag aggaaaagtc
    18781 ttttataaag caagatattg gagcaaaaat atcgaattta aaaaataaag aaaaatttga
    18841 tgtttttgaa tatcattttt tgacgaattt atatttaaaa gatataataa ttgggtactt
    18901 taatataaat gataaatggg taccaaaaga caaaaaattg ctaaaagttt taaaaaaaga
    18961 aaataatgag ctttttgaac tagtgtcaaa agtaagtgaa aattatgact atcaaagact
    19021 tttagatgtt tataattata tttttaaaga aattgagaca aaagaggtta taaaactaat
    19081 cttctagtca caaatcaatg gggggagttt aaatgaataa agttgataaa gaaaagatac
    19141 aacatgcagt aagagaaata ctagaggcaa ttggggaaga cccagataga gaaggtctaa
    19201 tcgaaactcc aaatagagtt gctaggatgt atgaagagat attctctggt cttagtgaag
    19261 aaccaagaga ccatttaaaa gttctttttg cagatgaaaa acatgaagag ttagtattgg
    19321 taaaagatat tccattttat tcatgttgtg agcatcatct agttcctttc tttggaaaag
    19381 ctcatattgc ttatttacca aaaggaggaa gacttacagg gctttctaaa cttgctagag
    19441 taatagatac attggcaaaa agaccacaat tacaagaaag aatcactaaa aatgctgcag
    19501 atataataat ggaagaatta caaccttatg gtgtcttagt agtagtagaa gctgaacata
    19561 tgtgcatgac aatgagaggc gtaaaaaaac caggttcaaa aactgttaca tcagcagtga
    19621 gaggaatatt tgaaaaggac attgcgtcta gagccgaggc tatgagtttg attactatga
    19681 agtagcaatt tattatagat ataatcattt attgagataa aatttaattt aagggagtgt
    19741 cattaattgt gtttgattat ggtaaaagaa cttatataat gggaatatta aatgtaactc
    19801 cagatagttt ttctgatgga ggagatttta ataacttgga catagcaata caacatgcaa
    19861 aagatatggt tgaccaaggt gcagatataa tagacctagg aggagagtct acacgcccag
    19921 gtcatagcta tgtagactca gacgaagagc taagaagagt aatccctgta ataaaaaaac
    19981 ttaaacagga attggatata ccaatatcaa tagacactta taaggcagat gttgcagaag
    20041 aagcattaaa attaggtgtc actatggtaa atgatgtttg gggccttaga aaagataaaa
    20101 atatggcttc tgtaatagga aaatatgatg cagaagtgtg tataatgcat aatcaagatg
    20161 ggacaaacta tgacaaagat ataatggagt caataaaaga cttttttaag gtaagtatag
    20221 aaatggcaat gagttatggt gtaaaaaaag aaaaaatagt acttgaccca ggtgtaggat
    20281 ttggcaagga ttttgaacaa aatattgaag tattgagaag attaaatgaa ttaaaagatt
    20341 tagggtatcc tattttgctt gggacatcca gaaaatcagt aataggaaaa gtacttccag
    20401 ttgagcctaa aaaaagatta gaaggtacaa tagctactac tgttcttggt ataagagatg
    20461 gcgtagatat agttagagta catgatgttt atgaaaattt aatggcagct agaatgactg
    20521 atgcaatata tagaaagtag gtataatata atggataaaa tattacttag taatctagga
    20581 ttctatggat atcatggtgt tcttaaagaa gaaaattttc taggtcagaa attctttgtc
    20641 gacatggaac tttatattga ttcaagagaa gcaggtctta gtgacgatat aaataaatct
    20701 gtaagctatg ccgaggttta caatgttgtt aaagatatta ctgaaaataa acaatttaat
    20761 cttttggagg ctttagcgga aaatattgct gaagaagtct tgaataaatt tatacttata
    20821 aatggagtta tggtcagagt tagaaagcct gaggctcctg taaatggtat ttatgactat
    20881 tttggtgttg agataaggag agcaagagat gaataaggct tatttgggaa taggtactaa
    20941 catgggtgat agatttgata atttatcaag agcatgtgaa cttttgaaaa atagtgattc
    21001 tatatataaa gtaaaagaat caagcttata cgaaacaaaa ccatggggat atacagaaca
    21061 agcagatttt ctaaatatgt gtgtagaaat agaaacagag tttgaacctt atgagttgtt
    21121 agaatattgt caagaaatag agagagaact acatagagag agaatagttc attggggacc
    21181 aagaactata gatgtagacg tactattttt taatgatgta gtttccactg atgaaagact
    21241 aacaatacct catccaagga ttcaagatag agctttcgtt ttgataccac ttatggattt
    21301 aaatgaagaa cttataataa atgaaaaaac aataaaagaa catttaaacc tcctatctgc
    21361 tgaagaaaga gaagaagtaa aggagcttgt aggttatgaa agaaaaccta tttaataata
    21421 attcaaagat agagataaat acaaataaca aaaagataac tgtaaaaaaa gataaactta
    21481 taatagctgg accatgtgca atagagagtt atgagcaact tttggagact gctaaatttg
    21541 taaaaagtca gggagcaaat attttaagag gtggagcata caagccaaga acatctccaa
    21601 attcatttca aggattaaaa aaagaaggtc ttgaaatatt aaaagcggtt aaagatgaag
    21661 ttggtatggc tgttattaca gaacttatgg atgtaagaga tatggacgaa ctgtatagta
    21721 taagtgatat aattcaaatt gggtcaagaa atatgcaaaa ctttactctt ttaagtgaag
    21781 tgggaaaaca aaataaacct gttatgttaa aaagaggaat agcatccaca attactgaat
    21841 ggataggtgc ttctgaatat atagcaatag aaggaaatag taatattata atgtgtgaga
    21901 gaggtataag aacgtataat gattatacta gaaatacatt agatttagct gcggtaccca
    21961 ttattcaaaa ggaaacaggt cttcctgttg tggtagaccc aagccatgcc actggagtta
    22021 gatatttggt aaaacctatg tcactggcat cttttgcatg tggagcagat ggaataatgg
    22081 tagaagtaca tcctgaccca gaaaatgctt tgtcagatgg tgctcaatct ctttgcttta
    22141 atgaatttga agatttaatg aaatcaatta ataattatta aaagaggcaa gagcctcttt
    22201 tttattcgta atcgttgcta aggtaaattt ataagttata atttgatagt tagattattt
    22261 aatataaata aagatttaat attgatagaa ttaatataaa aaataaaaat tttttgatta
    22321 ataagaagga tatcaataaa aaaagtcgaa ttgtgtatat tgagtgattg acaaatttag
    22381 aatatagtgt agaatagaaa tgttaacaca aaaaattaca ccagaaggtg ataatatgaa
    22441 tgatataaaa gacattattg aagaaataaa agctagatgc gatatagcaa gtataatatc
    22501 tgaatatatg aatataaaac aatctggagc taattacaaa ggattatgtc catttcatgg
    22561 cgaaaagaca ccttcttttt atataaatac gtcaaaacag atttataagt gttttggttg
    22621 tggtgaaggt ggagacgtaa taaattttgt catgaaaatg gaaaatttgg attttatgga
    22681 tgctgtaaaa attttagcaa ataaatgcgg tatagaaatt aacactaaca tgaatgaaga
    22741 aactaggatt aaaatagaaa aatctaagaa atttcaagat attcatacgg aagcagctag
    22801 attttatctt tcaaacttat tgggaagcaa aaatcttggg tatgaatatt taagaataag
    22861 aggactagat gataaaatta ttaagaaatt tggtttagga ttttctttgg attcatggaa
    22921 ttcacttatg aatactctaa tcagtaaagg atataaaaaa caagacttat tggagtgcgg
    22981 tctaattgca aaaaatagag atggaactaa ttgctatgat aaatttagaa atagagttat
    23041 gtttcctata ttcgattata gaggaaatat aataggattt gggggaagag tattagacga
    23101 ttctttgcca aaatacttaa attctccaga cacgttaatt tttaataaaa aacagaactt
    23161 atacggatta aattttgcta gaaaaaattt agaaagtaaa actatagtct tagttgaagg
    23221 atatatggat ttaatttcgc tttatcagta tggtataaaa aatgtagtag caacccttgg
    23281 aacagccttg actgaacaac aaggcatttt aataaagaga tatgcggaca ctgctattat
    23341 atcttatgat tctgatgaag caggaataaa agcgacttta agagctatag atatacttac
    23401 taaattaggt atcaatgtaa aagtgttaga cttaaaagat gctaaagacc cagatgaatt
    23461 tgtaagaaaa tacggactta gtgattataa aaaagctatg gatgtttcta ctcactacat
    23521 aaaatataaa atagatcatc ttaaaaaaga atttaacatt caaaaagatg aagaacgagt
    23581 gaagttcgca aaagaagctt ctaaaattat aaaacaattg acaagtcctg ttgaaattga
    23641 tttttataca aagtatttaa gcaatcaaat agatattaat gtcgagtcta taaagagaga
    23701 agtttatggt aaaaattaca ataagccata taataacaaa aatcaaaaga aaatagaaga
    23761 aaaagtaatt gaaaaagtcg aagtcagaca agacggaaaa caattagttg aagaaactct
    23821 aataaaaata atgttagaag acaaaaaaat tagagaaata gcgttactta aagtagaaga
    23881 aagtgatttt ttattgaaag aaagtaaaga aattttaaat tacatgatta aaaatcaaga
    23941 attggacaaa ataactattg acaaattgaa aagtttaaac atatccgaag aatacttaaa
    24001 agaattaaat tcaatttctt tgaatagtat aaacttagag aacacaaaag aaatagaagg
    24061 aataataaca aatatcagaa aaaactcatt agaagagcaa attaatagct tgttgagaga
    24121 acaacaagaa ttagaaaata ataatgatat gaaagaggta gatggtagag ttatggaaat
    24181 tgctttaaaa atagttgaga taaataaaat tttaaagagc ttgtagagaa aggaggtttg
    24241 agtgtggaaa ataaatcgaa taaaaaagag ttaaaaaagg ttactgctaa gacattaata
    24301 gaaaaaggaa aaaagcaagg ttctctgaca cttgcagaga taatggaagc tttttcggag
    24361 actgaacttg ataaggatca agtagaaaat ctttatgaga ctttaggaaa tttgggaata
    24421 gaaataacag aaacaaaaaa ctataaagct gatatagatt tttcggttgc tgatgacgat
    24481 ttaagtatag gccacttaga tgaagatgca gaggcaattt cacatgatga ctcttctgca
    24541 atagaaatag aaactgtgga tttatcttta ccaaaaggga taagtataga tgaccctgta
    24601 agaatgtact taaaagaaat aggaaaaatt cctctactta aaccacatga agaagtagaa
    24661 tttgctagaa ggatgcacga aggtgatgag atagcaaaac aaaggttagt tgaagcaaac
    24721 ttaagactag ttgtaagtat agcaaaaaga tatgtaggaa gaggtatgct tttcttggat
    24781 ttaatacaag agggaaattt aggtcttata aaagcagtcg aaaaatttga ctatacaaaa
    24841 ggatataagt ttagtactta tgcaacatgg tggataagac aagctataac acgtgctatt
    24901 gcagaccaag ctagaactat aagaatacca gttcatatgg tagagactat aaataagcta
    24961 ataagagtat caagacaact acttcaagaa cttggaagag atccaaaacc agaagaaatt
    25021 gcaaaagaaa tggaaatgac agaagataaa gtaagagaaa taatgaaaat agctcaagac
    25081 cctgtgtctt tagaaacacc aataggagaa gaagaagata gccatttagg tgactttatt
    25141 ccagatgatg atgctccagc cccagcagag gcagcagcat attctttatt aaaagaacaa
    25201 atagaagatg tacttggttc attaaatgat agagaacaaa aagtattaaa gcttagattt
    25261 ggtcttgagg atggtagagc cagaactctt gaggaagttg gaaaagagtt tgatgtaact
    25321 agagagagaa taagacagat agaagcaaaa gcactaagaa aactaagaca tccaagtaga
    25381 tcaaaaaaac ttagagatta tttagactaa aaagatacat cattgatgtt tttgtgataa
    25441 atgccatgag ataagtgata tctcatggct attttgtagt aaagatttaa ttttattttt
    25501 gtagttattt cagtctaata tctataactt ggattaaatc ctataaataa agggaattga
    25561 aaggagaatt atatttgaaa ttaacagata gattattgaa aatagcatca ttagtgtcag
    25621 atggtaaaaa aatagctgat atagggacgg atcacggata cataccagta tatttgttaa
    25681 aagagggaaa agtaccattt gcagtgctag cggatgttaa taaaggacct cttgacaatg
    25741 cacataaaga agtcatacag aacaatcttt tagataaagt tgatttacga ttgggttctg
    25801 gtatagagat acttgaaata ggggaagtag aagaagtgat aattgctggt atgggtggaa
    25861 tcttgataag tgagcttctc gaggctaaaa aagaagtagc acataatgta gaaaaattaa
    25921 tattacagcc aatgcaagca caagaagaac ttagatatta ccttttaaac aacggatatg
    25981 aaattttaga agaagtttta gtgagagaag attttagaat atatgagata attgttgcca
    26041 aatatacggg taaaaatact ataatagaag atgagatata ttatgaagtt ggaataaagc
    26101 ttttagaaaa caaagattct ctttttaatg attttataga aaaaaagata aagacatata
    26161 gttctatagt taataaatta gaagggaaaa atggggaagc tatagataag aagagaaagg
    26221 aaagcgaagt tgcaataaaa aaacttgaaa atttaataaa ataaatagga ggtatatatg
    26281 ctattaaaga gtctaactag aaaaattgaa aaaaagtatc ctctaaactt agcagaggat
    26341 tgggataatg taggtcttat agttggagat tttgatatgg atgtaaaaaa ggtattggta
    26401 tctttagaag ctaatgaaga tgttattgat gaagctatat ccaaaaatat agatttaatt
    26461 gtgacacatc atccttttat atttgggaaa attaataaaa ttaatagtgg agatttaaaa
    26521 ggcagactta tccaaaaact tattaaaaat gatatatcac tttattctat gcatacgaat
    26581 tttgatatag catttgatgg tctaaatgac tattttatgg aaattatgga atttggtaat
    26641 tcaaaggttt tagatataac taaaagcgaa aatttgtata aattagcagt atacgttcca
    26701 cataactatt cagatgaact tagaaaggtt ttaagtaatt caggagcagg tcatataggg
    26761 aattatagtg attgtacgtt ttcaatagaa ggtgaaggac aatttaagcc attagaaggc
    26821 tctaatccgt ttcttggaag tgtaaatgat atagaaactg taaatgaagt taaaattgaa
    26881 actgtagtac cacagaagct tttaggtggg gtaataagtt ctatgttaga tgcacatcct
    26941 tatgaagaag ttgcatatga tttgtacaag ttagaaaata aaggtgagat atttggacta
    27001 ggtagaataa gtaaacttga taaaagtatg acattagaaa gtctttcaag gaaaataaaa
    27061 gaaaaattaa acatgaaaca cataagagta gttggaaacc ttagtacaga cataacgaaa
    27121 gtagccgttg taacaggagc tggttcagaa tttgtaaaaa aagctaagag acaaggtgca
    27181 gaagtactaa ttacgggtga tgtaaagtat catgaggcac aagatgcact tgatattgga
    27241 atgtgtatag tggattgtgg tcattttgat acagaagata tattcaaaaa tgttatgaag
    27301 agatttttag atgaatttag tgaaattgaa gtaataaaga gtaatgtata tttgaatcca
    27361 tttagtacta tttagcagtg tttgataata aaaatactat ataaaaaata aatttgagtt
    27421 taattagggg gataatgatg aaagtagcag taatttttca tagtgtttgt gggagtacat
    27481 atttattagc tagagaatat aaagaagcgc ttgaagaaat gaatatcgag gtagatatat
    27541 ttagagtaag tgacgaagtt gcaaaaacac ttcctcaata ttatttgata aattccaaag
    27601 aatacaaaga cgaatttgaa agtataaatg taattaaatc tggaaaagaa atattagatt
    27661 atgatgctat atttatgggt tcaccaacat attatggaaa tgtatctgga ccaatgaaga
    27721 tgtttatgga tagtttttct gatatttggg tgggtgctcc tttgagtggt aaaatatttg
    27781 gttgttttgc tacagcaggt tcacaacatg gaggaggaga attagctttg caagctatga
    27841 atatatttgc ccaacatatg ggaatgactc ttttgtcagt tccatgttca gtaagaggag
    27901 gttatccagc gtatggaata cttcatatag caggagataa ttctgatata agaccaaatg
    27961 atgatgccaa gataggtata agagattacc taaaaagact taacatataa gaatatcata
    28021 taaaaatacc taaaagcgac tgtatatact tttaggtatt tttacttaat tataaacgtg
    28081 aagctttacc agaaatatag tcaatttcta ttttaacaac agttgttaag ttcatatcct
    28141 tctctatgta cttcatgcct tcttcaaaga atcctttaga atattttttt attatttcta
    28201 caagagcatt tttcttttct tcattttcta cagtgcaagc ttttccaaaa actatagcac
    28261 tagaataagt agtactaaat ttatctggaa taacactttc attagcaact actaaaaaag
    28321 aaactttgtt gtttttcgat atattatcta acttatgtcc atttcttgca caatgaaagt
    28381 aaatagaatc attaaagtat acataattta cagcaatccc ataaggataa ccattttcag
    28441 atatagtgga aaaagtacca aattcaccat tttttaatac ttcaacagta tcttcttttg
    28501 tcatttctct tttttttaat ctcatttctt taaacataat atcatccttt ctaattttga
    28561 tgcttaaatt aaattttaga acatttataa cgattatgct atgtttttta aaaattttaa
    28621 gaaaaaatat agtttaagat aaaaggtagt gaaagagaag ctatttggag gtttatttat
    28681 gatattattg ttagagtagt acaaacaata ctaataaatt taatagctga tttcttgtta
    28741 tttaaggtag taagcatgtc tataagaagg ggaagttatc gagggtagaa gttgttattt
    28801 ataacgatat tacaaagaaa atattattat agaaaataat aaatagtaaa gaggtatcaa
    28861 gttgaatata atacttaata cctcttcaaa attaaattat aaaagtgtta tgggaattgc
    28921 cctatacatg gtaaggagat aataaagaat gtatctttaa agtctcaact aaacagcaaa
    28981 atttatccaa caaacataca ataaatgctt ctttgtatct aggtggctta acagtaagtg
    29041 gccacatgtg ttttaaaatt atgtccgttt ctttttcatt tatttgaaaa aataaagttg
    29101 cattttttaa tgcttctcta gggtgagtaa atccatgtaa tccttttcta tcacctttgt
    29161 aatgccaatc ataaagaaaa aaatcgtgta gtaaagctcc tctaataaca ctttgaatat
    29221 caacacttaa gtgcaatttt ttacataata gataactata atatgctact gacaaactat
    29281 gttcaagaca tgaaacattg ccatgatgag gaaatgttct cataagttga actttttcag
    29341 aagaaagtat ctcagaagca cattcattaa aatatttgat ttcagctttt gacaacatta
    29401 tgaaaatctc cattcttagt aatatataaa tactatactt caacgataat atgaaatatt
    29461 tcttatccta caaaatataa tacccaataa tacaagtaag atacctttta tggtatttta
    29521 aagtaagaga gattttatta ggctaaacaa tgaaaaaatt aaaagtaaag ctatagcaat
    29581 atatagtatt tttccaaaag gattgccaaa atttatagtc catccaatac caaacctttt
    29641 attaaccatt aatgatgggt catttggatt attatacatg atgcctgcta tccaatactt
    29701 ctcatcttct tctggagaat aagatgagtt gaattttaaa tttggggaat taatatatgt
    29761 tgctattaaa taaattataa ctaaaagcgt tgtaatatcc atagcagttg atagttttgt
    29821 ttttattgat aaaagagtag taaaaaactg tgttgtcatt ataatcatca atattaaaaa
    29881 caaataacca attttattta aatatttaat gttttcaact ctgctttctt caattctatt
    29941 tgtatctatc ttaatccttg actttatgga tccaatagaa gttatatata ataaaaccat
    30001 taaacaaaag tcagtaccga ttaatttaaa tacattaaga aatgatttat ctataaaaac
    30061 atcaggagac ccatttatgt tccaatgagt ggcaattgag tctggcaatt tattataatt
    30121 aaatgctata aaaatactca ttccaaaagt aaatagtatt ggtattaaat agattatttt
    30181 aaattttttt attattttat ttttcttatt tataaaatcc atatctacta ttaattttga
    30241 atctatatct atatgaccta atttagaata aatttctgat ttaagcttct tagctttttt
    30301 atgtgtatga atatatagta tactttcata taataaaaaa cctataatac caacaaaata
    30361 ggaaagttcc aatttattaa atgtaaaaac cagaataaaa actaatataa agtctataat
    30421 aaaacctaaa gatagtagtt ttttaaattt tttatctaac tttataaagt aattataatt
    30481 tttatgctct tggtctatag aaactccata gatgtgtttt ttacctacaa aatagggcat
    30541 aaaataaaat gtagcataca tcaaaattag aactggaaat attgtaaata gacattcaat
    30601 cttactcata atatttcacc tcatacttgt caaataaatt tgaacttaat gacaaaaatt
    30661 cttctttact aatacctgat aaataagatt ttgccaccaa taattccaag tcaagtttat
    30721 ttttttgacg agttgtatta tctattttca ttggtagttg agctacaata gcaccttttc
    30781 ttctatcaat atttaagaat ccctcttctt ttaattcatt gtaggacttg ttcactgtat
    30841 gtaaatttac accaatattt tcagccatac ttcttacaga tggaagtgat tcatttattt
    30901 tcaattctcc tgaagctata gccttagtta tttcttcttt aatttgtaca taaatagatt
    30961 tatcactatt aaaatctaaa tttaaaacca taaaaaacct ccattatgct ttataaatga
    31021 aaacgattgt tatatacata gtataacata aaaaataata acaaacacaa aatataagtt
    31081 tatattttgt aaaaattgta atttaaaaaa taacataatt tttattaaaa taatacagat
    31141 actatttata gatagaaata aaaattagaa aggagaaaaa atatgttaac atatttaatg
    31201 aaaagacaaa tgcataaaaa agatgatgga atgagtaaac caatgatgat tacaggagct
    31261 gtaattacag gtgtaggagc atatgctgta tacaaaatgg taaaaagcca taaatctaat
    31321 tctcaaatgg tcaatacaaa ttattatgga aatagcgatt atgatgatta taaatatgac
    31381 gaatttgatt atgactgtga ttgtagagac acaaaacaag attatgaata tgaagaatta
    31441 aaaaaaaaag taaaagaatt taattcaaga agaataaatt gtgaaaattg tccacatgtt
    31501 tctcaagaag aaatgatgca atatgtaaat agtgttaaag atgtaaagga agagattgat
    31561 ttacatgaag atgctaaaaa agataatatt tacaaagaag atgaatacaa aaaatatgaa
    31621 gaagaataag taatattttg tataactgaa caaatagtaa tgtaaaatta tttaatatct
    31681 agttttaaaa tgtaagttgt aacttaactt gaaagaatca gtatc
//
LOCUS       DAGSHT010000024        31298 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGSHT010000024 DAGSHT010000000
VERSION     DAGSHT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.025"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(176..436)
                     /locus_tag="MJB72_001045"
     CDS             complement(176..436)
                     /locus_tag="MJB72_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBY8262170.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            653..1936
                     /gene="hflX"
                     /locus_tag="MJB72_001046"
     CDS             653..1936
                     /gene="hflX"
                     /locus_tag="MJB72_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBY8262171.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            1944..2621
                     /locus_tag="MJB72_001047"
     CDS             1944..2621
                     /locus_tag="MJB72_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBY8262172.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2707..3150
                     /locus_tag="MJB72_001048"
     CDS             2707..3150
                     /locus_tag="MJB72_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY8262173.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3455..4099
                     /locus_tag="MJB72_001049"
     CDS             3455..4099
                     /locus_tag="MJB72_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBY8262174.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4185..4562)
                     /locus_tag="MJB72_001050"
     CDS             complement(4185..4562)
                     /locus_tag="MJB72_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBY8262175.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4562..4948)
                     /locus_tag="MJB72_001051"
     CDS             complement(4562..4948)
                     /locus_tag="MJB72_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBY8262176.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5127..5882
                     /gene="nadE"
                     /locus_tag="MJB72_001052"
     CDS             5127..5882
                     /gene="nadE"
                     /locus_tag="MJB72_001052"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBY8262177.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            5965..6684
                     /locus_tag="MJB72_001053"
     CDS             5965..6684
                     /locus_tag="MJB72_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY8262178.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6851..7855)
                     /locus_tag="MJB72_001054"
     CDS             complement(6851..7855)
                     /locus_tag="MJB72_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBY8262179.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8121..9017
                     /locus_tag="MJB72_001055"
     CDS             8121..9017
                     /locus_tag="MJB72_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBY8262180.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9138..10400
                     /locus_tag="MJB72_001056"
     CDS             9138..10400
                     /locus_tag="MJB72_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262181.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10476..11669
                     /locus_tag="MJB72_001057"
     CDS             10476..11669
                     /locus_tag="MJB72_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBY8262182.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISDAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12298..13092
                     /locus_tag="MJB72_001058"
     CDS             12298..13092
                     /locus_tag="MJB72_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBY8262183.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13187..14527
                     /locus_tag="MJB72_001059"
     CDS             13187..14527
                     /locus_tag="MJB72_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY8262184.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14664..15896
                     /locus_tag="MJB72_001060"
     CDS             14664..15896
                     /locus_tag="MJB72_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBY8262185.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELISILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            15936..17078
                     /locus_tag="MJB72_001061"
     CDS             15936..17078
                     /locus_tag="MJB72_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBY8262186.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17089..17883
                     /locus_tag="MJB72_001062"
     CDS             17089..17883
                     /locus_tag="MJB72_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY8262187.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17894..18916
                     /locus_tag="MJB72_001063"
     CDS             17894..18916
                     /locus_tag="MJB72_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY8262188.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19015..20391
                     /locus_tag="MJB72_001064"
     CDS             19015..20391
                     /locus_tag="MJB72_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBY8262189.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20723..21067)
                     /locus_tag="MJB72_001065"
     CDS             complement(20723..21067)
                     /locus_tag="MJB72_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262190.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21555..22565
                     /locus_tag="MJB72_001066"
     CDS             21555..22565
                     /locus_tag="MJB72_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY8262191.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            22701..23189
                     /locus_tag="MJB72_001067"
     CDS             22701..23189
                     /locus_tag="MJB72_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8262192.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23365..25293
                     /locus_tag="MJB72_001068"
     CDS             23365..25293
                     /locus_tag="MJB72_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8262193.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            25470..25880
                     /locus_tag="MJB72_001069"
     CDS             25470..25880
                     /locus_tag="MJB72_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBY8262194.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            25902..26204
                     /locus_tag="MJB72_001070"
     CDS             25902..26204
                     /locus_tag="MJB72_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBY8262195.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26421..27113)
                     /locus_tag="MJB72_001071"
     CDS             complement(26421..27113)
                     /locus_tag="MJB72_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBY8262196.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESETTKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(27139..28326)
                     /locus_tag="MJB72_001072"
     CDS             complement(27139..28326)
                     /locus_tag="MJB72_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8262197.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            28662..30461
                     /locus_tag="MJB72_001073"
     CDS             28662..30461
                     /locus_tag="MJB72_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8262198.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFD"
     gene            31107..>31298
                     /locus_tag="MJB72_001074"
     CDS             31107..>31298
                     /locus_tag="MJB72_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8262199.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLK"
BASE COUNT        11839 a         3609 c         5841 g        10009 t
ORIGIN      
        1 taaatcttta tcaataaaaa tatttcaatg ttaaatagag ggatattttt atttccaaaa
       61 taagataata aaaaaggatt agattaaaag ttatacaata aaaatagctg tgcaataata
      121 tttagactat gaaagtagtt gaaaaatatt tatgcacagc tatttataat agaatttact
      181 ttttactagt gattttaact aaatcttgaa atttactcat aatgtaattg taattatctt
      241 ttttatgagg taatatacaa ctactgcaat ctttgatacc tttatctgta tacttaaagt
      301 ttccaccaca gttttctcct aaagcataca aaggacaata gcaaaataga cagttaaact
      361 cttctggttt attagtttta tgacaaggga aaaattcgca atctttgtga ttaaaaaact
      421 tataattttc gcccatattt tcctcctaat taaataaaat ttaaattttt tatactatat
      481 aagattttgg tgataatata attatactaa ataattttat gaatagtcga taatatagta
      541 ttattaatca tagtattaat ggtataatta gtcaacagaa aaataatgta aaaataatgt
      601 aagtgtattt tgtaattaga atatgtttta taacaaaaaa tgaggtgatt aagtgaaaga
      661 aattcaagaa aaagcacttc ttgtaggtct gaatttgact acaatagtga aaaaaaatga
      721 tgatatagac actaatgaat ctatggaaga attaaaagaa ctaacaaaag ctgctggggc
      781 agaagttgta ggtagcttaa ttcaaaataa acactctgta gatgcagctt attatatagg
      841 aaaaggtaaa gtagaagaaa taagagccta tagtgactct ttagatgcta ctttagtaat
      901 ttttaatgat gaattatctg gtgctcaaat cagaaatata gagaatgttg ttggaagaaa
      961 agtaatagat agaactactt taattttaga tatatttgct caaagggcac ttagcaaaga
     1021 aggtaaatta caagttgaat tagcacaatt aaaatataga ttgccacgac tttatggaat
     1081 gggtggtgag atgagtagaa caggtgcagg gattggtact agaggtcctg gagaacaaaa
     1141 actagaaata gataagagac atatacttaa taaagcagca gatataagac gtgaacttaa
     1201 agaagtaaaa aagaatagag agacacagag agttaagcgt ttaaagtcaa atataccgat
     1261 tgtagcactt gttgggtata caaatgcagg aaaatctacc ctgttgaacg aattaataaa
     1321 gacacacaag gattatgaac aagaaaaaga agtttttgta aaagatatgc tttttgcaac
     1381 tttagatgta actttgagaa aggctctttt gccgaataaa aaagaatttt tagtagtaga
     1441 tactgttgga tttgtaagta aacttcccca tgatttagta gaagcgttta aggcaacatt
     1501 ggaagaagta cagtatgcag atttaatctt acatgtaatt gatgcaacaa atactagtta
     1561 tgaattgcaa aaaagtacga ctgaaggtgt attaaaagaa cttggtgtaa atgataaaaa
     1621 acatatactt gtatataata aagtggacaa gctagaattg gatatatatc ctaaaagtca
     1681 agaagatatt gtgtatattt ctgcaaaaca aggcataaat atggataagt tattaaatat
     1741 gatagaaatt gctttaatgg aaaatactta ttcagtaagt ttgatgttac cttatgagag
     1801 aggtgatata tttagtagaa taaaagataa atataatgtg gagaattttg agtatgggga
     1861 aagtggtata acgctagatg tagatttaga tgaagaagat tttaatatct atagagagta
     1921 catactagaa aaataggttg aatatggata taaactggga aaatatagat aatcttgaag
     1981 attattttat aacctatctt ctttataaag aatctaaaac agtatctcaa attagtaaaa
     2041 ttagaaatat ttcaagtact gaagcaagtg agcaacttat acaagcaaag ttaaaaataa
     2101 aggaaatgca aaaagatgat tttgaggcat ctaaggatat tttggataaa tttctagaac
     2161 tagataaaat taagagactt gattttatgg atagcttaga tgacgaaaaa atggtatact
     2221 ttaagagaaa ggttttcaaa agaatccttg tagaaaaaaa tgctgaagat ttaattgttt
     2281 taatttgggc gacaggtgag ctaaaagatg ataggttttt gaaattgctt catcagctca
     2341 caaaccacag acactcagat attagaagaa taacttattc agcaatcaga aagattgaat
     2401 caccaagtag tagagaagta ctgcaaaaag gtctctacga caaaaatgca cagactaggc
     2461 agtactgtgc aaaagctctt tcaaaattgg gagatgaaaa cagtttaaag attttacagc
     2521 agttgaaaga caagaataag aattttgaaa aagaatatgt gcttagagct tatgacgaag
     2581 ccattaagtc attggaaaat gcaaaaaatt tgaaaaagta attttttatt aaagcaattt
     2641 gtttacttaa tgatgtattt ctaaagttta acaaggtaat aaataaatta atgggagggg
     2701 tttcaaatga tagtaagacg ttgtgcaggt ggagtagtgt tttatgctaa taaagtactt
     2761 attgtaaaaa atgatagggg tgaatggaca cttccaaaag gaaagatact aggaggggga
     2821 cttccatatg agagtgctgt tcaaagagtt aaagtagaaa ctggcataga tgctaaaatg
     2881 atagatgtgg ctggcgatac tatgtatgaa tttttctcaa gaagtagaca acaagaagtt
     2941 tgtaatgcaa taatgtggta tgttatggaa gcttgtaaca ctgattgtgt acttgctcct
     3001 gaatttcaag aaggtggatt ttataaagta aaggatgctt tagaaatgtt atcacatcat
     3061 aaagagcaag ctttagtaga agtatcttat aaaaagttta aggaattaaa gaaattagtt
     3121 tcagagggcg atgatgttcc agcaaattaa ttgtgaattt agaggattct gaggaaggga
     3181 tagccccttc cttttttaat tgaagcagtt ttaagcgtaa attatatagt ctctttaaac
     3241 tatgatttat caatttagaa atttagtagt ttatatttaa aatcagtaag atatatttaa
     3301 aataaaaatt aaaaagtata caattttatt atataattgt cttatgatgt tgattaaaga
     3361 attattaaaa gtccatttat ttttaaatag aataatttta taaatatata tttgatgtgt
     3421 atattagaaa atgattaaaa taggaggaga tacaatgagc aattacagga cattacatga
     3481 atttggaacg gatgaaataa cgattgaaaa gtctgttttt attggatatg ctaagcctat
     3541 aaagagtgaa gaagaagcat tagagtttat aaatgaaata aagaaaaagc ataaagatgc
     3601 aactcacaat gtgtgggctt atactgtagg taaaaatatg aatatacaaa gatatagtga
     3661 tgatggagaa ccacaaggaa cagctgggat acctacatta gaggttataa aaaaagaaga
     3721 tttgagagat gtagcggttg ttgttactag gtattttgga ggaacaaaat taggtgcagg
     3781 tggtctagtt agagcttata caaaaggtgc taaactagga cttgaagcag gtaagataat
     3841 ttataaggta atgtatcaag aagttaaggt taagatagat tatactcaac ttggaaaagt
     3901 gcaaaatgag ttaatgaatt tggggtattt tataaaagat actgtatatg aggataatgt
     3961 tgaaatagta gtatattcaa gattagaaga tgtagagaaa ttatcagaaa aaatgattga
     4021 tataacaagt ggaacaggaa aaatagtttt aggagaggaa ttttatttgt ctgaacaaaa
     4081 tggagaaatt ttaatataat ctaaaaggtt attagaatag ttaatacctc aaatagccaa
     4141 ttagtatttt taagtgaaaa gccattaaat tgatttaatc taaatcaatt taatggcttt
     4201 ttatcggatt ttgcacagaa tccttttttt acttcaaata aaccgactaa aagtaaaaat
     4261 atcccaaata ttttttttaa tatatctgaa gacagataat tagctaatag tgaaccaaat
     4321 acagctccta aaaccccaaa cacagctata ggtataacca atttaaaaat tacattttta
     4381 ttttttatat gtatagcaag tgctataagt gtcataggga tagatgaaag taaatttacg
     4441 ctttgtgcta ttttagtgtc tatgccagca agtaaaacga gtgcaggtat aaggattgtt
     4501 cctccaccca ttcccattcc tccaataatt cctgcaaaaa atccaattag agcaaataac
     4561 attaaaacac catccttaat gctgcaatta tcataattat tccaaaagaa attctaagaa
     4621 actttccagt aaccttattt agtaatttag ctcctataat accaccaaga atacttccaa
     4681 ctgcaacttt aattgttaag ttaacatcat aagtaccttt agatacatat aaaacagaac
     4741 ttgctgtcgt taagaatatt attatagaaa gagcagttgc atgagattta tgttcttcta
     4801 ccttaacgat gtcatttaat attggaacta aaagagtacc tcctccagaa ccaaaaactc
     4861 catttataaa tcctgtaaaa actccaatta tcgtattttt taagttaaaa tcaaagtatt
     4921 ttttctttaa atcatcagtt tttttcataa aaatcacctt ctgaaatagt tttaccaaaa
     4981 taggcaaatt atatttatat aaaataagat taaatataat ctttcatgtt tgtttaattt
     5041 tgcataaaaa aaaacatatt aaaacaacat attaatatat ggtataatat attaaaacaa
     5101 agtagcaaat ttgagaggag ataacaatgt caaatataaa aatacaaata gacaaaactg
     5161 tagaatggct tataaataag gttaatgagg caaatgcaaa aggattaatt gttggtgtat
     5221 ctggaggaat tgattcggca gtagttgcta atttaattaa aaaggcattt ccagaaaact
     5281 ctatgggagt tataatgagc ataaaaagta atcctcaaga tagagaagat gcacttaagg
     5341 ttatagaggg atgtgatata gaataccttg atttagacct tatagaacca caaagtgcta
     5401 tattagacat ggtagtagga aatttaaaag ataaacattt atatagagaa gaatatttaa
     5461 agatgacaga tgcaaactta agagcaagag taagaatgag tacaatttat acaattgcta
     5521 acaatctagg atatctggtt gttggtacag acaatgctgc agaaatacac acaggatatt
     5581 ttactaaatt tggtgatggt ggagttgata ttttacctat tgcaaattta actaaaggag
     5641 aggtgtatga atgggcaaaa gagcttggtg ttcatgaaga tttaataaac aaagctcctt
     5701 cagcaggtct ttgggaaggt caaacagatg aagatgaaat gggaactact tacaatatga
     5761 tagatgctgt attagaaggt agattggatg aagttcctaa aagagaccaa gagataattg
     5821 aaagacttca tagattaagt gaacacaaga gaaaaacgcc tgcacaacct cctaagttct
     5881 aatttactat ttataagatt tgtgataaaa tttacaagtg atgaaaatta gaataattaa
     5941 ttgtacgtat taaggagtgg aaatatggga cgtataggta atataattaa tagaaaaggt
     6001 aaacaagatg ctcaaagagc aaagatattt actaaacatg ctagagcaat agcagttgca
     6061 gctaaagaag gtggagcaga cccagagtac aatgcagcgt taaaaacagc tatagaaaaa
     6121 gctaaggcag ataatatgcc aaatgataat atagatagag ctatcgctaa aggtgctgga
     6181 gcaggtgctg gagaagacta tgaaactata gtttatgaag gatatggacc tggtggagtt
     6241 gctgttatag tagaaacttt aactgacaac aaaaacagaa ctgctggtaa cgtaagatac
     6301 tattttgaca aaaacggagg aaacttagga actagtggat gtgtgtcttt tatgtttgat
     6361 aaaaaaggac aaatattagt tggtttagga gatggagttt cagaagaaga attgatggat
     6421 gttgcattag aagcaggagc tgaagatttc ataacagaag aagatggtta tgaaataata
     6481 actacaccag aagatttttc tagtgttcgt gatgagttaa aagctaaagg atatgagttt
     6541 atatctgctg atgtgaagat gatacctcaa actacaactg tattgactga agaatctcat
     6601 ttaaaaatga tgaataaatt agttgatatg cttgaagaag atgatgatgt tcaagatata
     6661 tatcataact gggaagtaga ataaatagta taagcatatt gaaatttata agtattgaat
     6721 caattttaac atgtgataaa ttttatagaa taagaaacac taaaatagac atacgcacct
     6781 ctaacctata tttgcataat aatatgtaag cataggtaag gggtgttttt atatttatag
     6841 gttaagaaat ttaaagtaaa ttttgagctg gactcgattg tctataaata attgatgggt
     6901 atataacttg ttgctgaata ccatttatac gcccagttac tgtatcaata atcatcctaa
     6961 gactacgttc tgctgtctct tccattttta aatctataac tgtcaaagga ggaatacttc
     7021 ttgccattag tcgtgaaggt ccattacttg ctgcaaatat ttgaatatcc tctccaatta
     7081 caattcccga ttcatgaaat gcattcatca taccaagagc taccacatca tgattacaaa
     7141 aaattacttt ttttagtgaa ttttttgcaa gcattttttg agcaatatca tatccactat
     7201 ctattttatt agaacaataa tatatagaat ctgatagatg tattccatgt tctttacatg
     7261 tttttaaaaa agatttaccc cttaaagtca tacatgtaaa ggaagttggg ttgaatacaa
     7321 cagcaatatc atttttacca ttttgaatag catgtaaagc tgctatacga ccagtttcct
     7381 cattatcaat agatacactt gaaaaaccag gtatttcacg atttacaaca actacaggaa
     7441 ttttaggttt attttttact agatattcta tatcatcagc attgatacct gttaaaacaa
     7501 ttccatcata ataattattt gcaaaaaagt attcatcatc acatagatga ccaactttgt
     7561 aaggtcgtat gatgatattt atgtttaaat gttctaaaga aattatgtgg tttattgctt
     7621 gaagtatatt ggttgatgcc aactcaaatc cctcatcctg ccaataaaaa gctattttaa
     7681 tattattatg tttaatagga gtagagcgtt tatatccaag cttatcagct gaatttaata
     7741 taagctcttg tgtatgtagc ggaatcctgc gttcatttcc tttaccactt aagacgtacg
     7801 agacagttgc tgaagaaaga ccacagtctt ttgcaatatc tttgattgtt gccatgataa
     7861 aatcctcctt aaatagttgc ttaaatttgc ttataccaat aatcttaaca tatattcaac
     7921 taaagaacaa tattctacaa ggtgcatatt ttacaggatt agaataaata aatttaaaaa
     7981 aacttcttac ttatgttgac ataatttagt ttataggatt ataatatgct taaataagct
     8041 tatgattaac ttaaattaag caaagttaag caggaacatg caatgctagc ttgcaatgtg
     8101 actgtaaagg gggaaaaggt atgagtaagg taaatatatt tgaagttggt cctagagatg
     8161 gatttcaaaa cctgaaagag tttttagaaa ctgataaaaa gcttaagatt attgatggtc
     8221 ttattgcttc agggattaaa caacttgaaa ttagttcatt tgtaagccca aaagcaatac
     8281 ctcagatgaa agatgcaaaa gaagtagcaa cttattgtgt agaaaaatat cctgatgtga
     8341 aactttatgc attagttcca aatttatacg gtgctaaagt tgcatgggag tgtggtataa
     8401 gagatatatc ctatgttata tctttgagtg aaactcataa taaaaaaaat ataaatcgta
     8461 cccatgaaga gtctattgat gagcttaaaa aaataatgaa tacttatcca gacatgaata
     8521 tatgtattgg aatggctact gtatttggat gtccttttga aggtttacca aaaattgaag
     8581 atgccataag atttgctaaa cgcctttggg aaaacggtat cagggagatg aatctagcag
     8641 atacaatagg gattgctgac cctaagcaag taagagaaac tattagtgca ttagttgaag
     8701 ctttaccaga gtgcagatgg caagtacata ttcatgatac tagaaatatg ggaatggtta
     8761 atactcttgt ggctattgaa tctggaatta ctactgttca gtctacactt ggaggattag
     8821 gaggatgccc atttgctcct ggagcatctg gcaatactgc aactgaagat ttagtatata
     8881 tgcttgaacg tatggggtat gatacaggag ttgattttgt taaacttctt gaattatcaa
     8941 agtatcagaa gagttttgta aatggagtat atagtggtca tcatgtaaat ataaagtctg
     9001 atagttgtaa ttgttagttc tattgattaa tcgattaaaa aaatatgctg attggtaaaa
     9061 tagatttatt gataaaaaat ataaattata aaatacatta tctaataaaa caaataaaaa
     9121 aattgagggg gattagtatg gatattatag ctttactttt atttttagga gttattatta
     9181 ttgcatttgt acgtaagaat aatgcaggta ttttggctct agctattggt gtcatagctg
     9241 ttagaatttt tggaatggat gataaagaac tttatggagc tataagcaac tcaatgttta
     9301 ctacacttgt tggtataaca ctactctttg caatagtgaa ctcaacagga gcattaaagc
     9361 ttgttgcaga aaaaattgta gctgcttctg gtaaacgtgt ttggcttata cctatagcag
     9421 tttttatagc aggatttgtt gttgctggtg ttggacctgg ggcaattcca gcacttgcaa
     9481 ttatcccagc aatggctgtt cctgtagcca taacagttgg ttataatcct gtaatgcttg
     9541 cacttatagg tgaatgtgga ttaatggcag gtcgtatgac tcctattact ccagaaggtc
     9601 aaattataac aaccgttgca gaaggggttg gaattgaaaa tgttatgcct actatcttag
     9661 cctgtcaaac tctttctaca attgtatttt caatagtatt gtttataatt ttcaaaggat
     9721 ataaacttaa aaaacctata aatgttatag atagtaagca aatagaaaaa ttcaatataa
     9781 agcagataat ttctctaata agcatagtag ttatgattgc ttttatcata atttttgata
     9841 tgaatgttgg gcttgcagct tttgcagttt cagctgtact tatttttttc aatattgctg
     9901 atgaaggaga atgtatcaaa tcaattccat ggagcacgat agtaatggtt cttggagtag
     9961 gtgcaatgat gacaatcgta gatgcagcag gaggtattga cttgatgagt aatgcattat
    10021 catcactgat gaatagtaaa acagctactc caattatgag tatttcagca ggacttatgt
    10081 ctatggttag ttctgcttta gcagtagttt atccaactat gatgcctatg tgtgttgata
    10141 tagctaaggc agttggagga gtagacccac tagcacttat tgctgcagtt ggtgttggag
    10201 gttctcttgc tggtgtaagc ccactttcta ctggtggggc attgatttta gcagctatgg
    10261 gttcaagtaa gaaagacttt aataaggaag aacaaaataa ggtttttgta cagcttttca
    10321 ttatgagtgc agttggctta ttggttattg cagtagtatc aatattatca tttaatgcaa
    10381 taacacattt aatgaactaa ttagaataac tgaaaataaa tcattaaatt taacttttaa
    10441 tattaatcaa tataatataa tttggagggg aagttatgaa aaaaggagca ttaagcaata
    10501 taaaggtact tgaccttaca agagttcttg caggtccata ttgtacaatg atgttggcgg
    10561 attttggagc tgatgttata aaaatagaga tgccaagaaa aggtgatgat acaagaggtt
    10621 ttggaccttt tgtaaatgga gagagtttat actatgcaaa tataaataga aataaaaaag
    10681 gtatcactct aaaccttaaa tcagaagaag gaaaaaatat atttttagaa atggtaaaac
    10741 atgctgatgt tgtagtagaa aattacagac caggtgtaat ggaaaagtta ggtctgggtt
    10801 atgatgagtt ggctaaagtt aacaatcaaa taatttatgg agctgtatca ggatttggat
    10861 gttatggacc atattcagaa cgtccaggtt atgatattat tgcacaggca atgggtggtc
    10921 ttatgagtat aacagggcaa gaaggaggag aaccaacgag agttggtaat gctatgggtg
    10981 atgtactagg tggaatgaat ttaactattg gtatactcac agcactcaac gctagaaaaa
    11041 taataggaaa aggacaaaga gtagatgttt ccctcgttga ttctgttgta tcgtctctcg
    11101 aaactggtat acagagatac tttgattcag gacaacctga acttatgggt aatagatatg
    11161 cttcagccta tccttatgac tcttttaagg caagtgatgg aagttttgta ataggctgtg
    11221 gtaatgacaa actttttgaa aagttatgta aaaaagttct ttttagagaa gaccttttaa
    11281 tagatgagag atttaaaaca aatcttaatc gttgtgaaca ttacaaggct cttaaaccag
    11341 aaatagaaaa atggtctgtt aagcatacaa taaaagaagc agttgactta atatcagatg
    11401 ctggagttcc tgctgctcct atctataatc ttgattgtgt tacaacagat gaacatatag
    11461 caaaagcaag agaaatgatt gtagatatta gtcatcctat tataggaaat atgaaagtca
    11521 atggaaatcc agttaagttg atgacaacaa aagctaaaat tgatatgcca gcaccatctc
    11581 taggggaaag taacgaggag atttataatg gttggcttgg ttttgatagt gataaaataa
    11641 aagatttaaa agaaaatgga gttatttgat taaaaaatag ctaaacacac ttgtgacttt
    11701 agtcatggga ggttcagtta gaataatatc aaaaaatatt aaatcaatac tgtaaatcaa
    11761 atatagaatt gtcagaagca tttaagatgg gaaaagctac ttagacattg agtctttgtg
    11821 gcttttccta ttttcattaa gaattttata ttatcagaat agatatgtac taaactaaag
    11881 tattaacaat ttaaattaat agaaaataaa ctacctaatt atgttgaata aagactactt
    11941 tagtgaaaac aaacttatag agatgctatt taaattattt taatggaaat atcaaaaatg
    12001 agactgcatt ctcaaaaatg agactgcgtt ttttaagtat tttctcgaaa atgagacaaa
    12061 aatattgttt tatgggtttt attgaagaaa aattcttctg tgaattacaa tttaatcaat
    12121 tttaaagttg aaaattatta aaaatttagt gaaatttatg tatatttcgt ttattttatt
    12181 aaaaataaca attttttact aaaaataaag aacttcaatg gtgattggca tagtacttgc
    12241 tattttatat taacaagaaa tgtagaaaaa ataacaatgt ataatggggg gaatatgatg
    12301 gaatctgtaa tggaaaattt gaacaatctt aaggtggagg ttaaggacaa agtctgtgtt
    12361 attactataa atagacctaa agctttaaat gctcttaata gtgacactct tagggaacta
    12421 tcacaagtta ttgatgtagt atcagaaaat gaggcaatat taggagttat tattactgga
    12481 gaaggtaaag tttttgtagc cggtgctgat atacgccaga tgcaaaacta taaaagtgag
    12541 gaaggcagaa aatatgcagg ttatgctcag ggaatttttg ataaaattga agctcttgag
    12601 aaaacagtaa tagcagcagt taatggatat gcgttaggtg gtggatgtga acttgctatg
    12661 agttgtgata ttagaattgc atcagaaaag gctatatttg gacaacctga agttaattta
    12721 ggagttattc catgttttgg tggaactcaa agattatcaa gacttgttgg aactggaata
    12781 gctaaagagt taatttttac ggggagacaa gtaaatgcag aagaagctaa atctattggg
    12841 cttattaata aagtggttcc aagtgattta cttttagaag agtctatgaa gatgatgaac
    12901 caaatagttg aaaaagcgcc aatagcaata agatatgcaa aagttgttat taacaaaggg
    12961 atagatatgg atttaaagaa tgctcttgaa ctagagaaag atattgcagg tttaactttt
    13021 gctacaagag ataagcaaga aggaatgaat gcatttatag aaaagagaaa gccagttttt
    13081 gaaaataagt agctattata actaaaattg gaacaatgta gaatatattc aaaatctaaa
    13141 tttcgtgaaa aaatacatat agtttaattt taaaaggagt taatttatga atacgggagt
    13201 aattataagt ttagtaggga ttatcatagc tctatctctt ctagttgtaa tggttatgaa
    13261 gggaataaat atttttatta tagcaattgt atgttcatca ttagttgcta taacaggaag
    13321 cctaaatgtg tatgatgcac ttaaagtgga ttatatgaca gggtttgttg gatttcttca
    13381 ggcaaatttc tttattttcc taacagggac attaatggga aaaataatgg agattactgg
    13441 tggagcaaaa tctattgcta agatgattgt aagatggatt gggaaggata aagcactact
    13501 ttctattcca atagcttgtg gaatattagc ctatggtgga gtaagtgttt tcgttgtaag
    13561 ttttgcagtg tttccgatag ctttagaaat ttttaaagaa gctgatttgc cacgaagatt
    13621 tataccagca gctttgactt ttggatgctc aacttttgct atggttgcac caggggcacc
    13681 acaaattcag aatattgttc cagcatcaac attagggaca gatattatgg ctggagcagt
    13741 aaatggtttt atatcatgtg cagtaatgtt tatagttgga agtacaattt tgtacaaaat
    13801 ggtttcaaaa gaaaaagcca atggtggtca ttttatagca catgaatcag atatatttga
    13861 tgaagcagaa gcaactaatt ctaagagaga aaatggtccc aatggtgtaa ttgccttaat
    13921 tccattgata gtgtcaattt taataattaa tgtaaaagtt aatggaaaag cgatagtacc
    13981 aattgaggtg ggagtattta ttggagcttt attagtatat ttattgttaa ataaatatca
    14041 agataatact aaaatagttg gacatgttgg tgatgcatgt aaaactacag tagtagcaat
    14101 atgtaatact tgtgctgtag ttgcatttgg ttcagtagta aaatcagcag ttggttttga
    14161 cttcatagtt aatgctatga ctaatattcc tggtcctcct attgcagggg ctgcagttgg
    14221 aactacagtt attgcaggta tttgtgggtc agcttcaggt ggactaggaa ttgcagctcc
    14281 acttcttgga ccagtatttt tagccaaggg tgtttccgct gcagcattaa ctaggactat
    14341 ggcaatttca tcagcagcat tagattcact tccacacaat ggatatattg ttactgtaac
    14401 aaatggtctt tgtaaagaaa cacataagga tgcctatatg ccaatattct gggtaactgt
    14461 tttaacccca tttattggaa ccatagtagc agtcctactg tttacaatgt tcccgggatt
    14521 gccataagag aaaagataaa aggtataaat tatattttga tgttataaaa tcaattttca
    14581 aaatatatac aataaatttc atgttttaat actgtaaata tgtatttaaa ataacaaatt
    14641 gaccatataa ggaggaattg aaagtgttaa ataaaccatt agagggagtt aaggttattg
    14701 acctaacgta ttttgtagca ggtccaggga cttctaaaat attagctgac tggggtgctg
    14761 acgttattaa ggtagagcca agctttggag acccaggaag aaagacaggg gcaacaatga
    14821 caatgccaat agatgattat aataatcctt tttacagcac atataattca aataaaagag
    14881 gtttgtctat aaatcttaag agtgaaactg gaattgaaat tatggataag cttttaagcg
    14941 aagcaaacgt atttgtttca agttatagaa caggagcatt aaaaagacta ggtcttgatt
    15001 atgaatcatt aagtaaaaag catccacacc ttatctgggg tcagataaat ggttttggcg
    15061 attttggacc tgctaaggat aatgcaggat ttgatacagt tgccttttgg gcaagaagtg
    15121 gggctatgct tgatatagca gaaaaagata caagtccaat aaatccacct atagcctttg
    15181 gagatgcaac aacttcttgt agtttatctg gaggaatatg tgcagctttg taccagcaag
    15241 ctaagactgg aaaaggacaa aaagtaatgg catctttatt tgggcaagca atatggaatg
    15301 catcatcttt attagcatct acacagttta gtgatgagta tccaaaaact agaaaaaatg
    15361 ctatttcacc agtaataaat tcttatcgtt gtaaggatgg aaagtggata ttcttaagta
    15421 ttttagagca tgaaagatat tttgaggcac tttgtagatt atttggaaga gaagacttaa
    15481 taaaagaaga gaaatttgca tcttcagtag aaagtaagaa aaatgccaca gaacttatta
    15541 gtattttgga tggtgaattt gcaaagttta ctcaaaatga gatggttgaa aaacttactg
    15601 ctgctgatat agctcatgaa aaaattcaac atgttaatga tgtagtgact gatgaacagg
    15661 ctattgctaa taactatata tatgaacata caagtaaaaa tggaaataag acaatgcttg
    15721 catctactcc agttaaattt ggaaatattg aagttaacat gacttgtgat gctccactta
    15781 taggagaaca ttcagatgag atattgagag aacttggata cagtgattct gatatagagg
    15841 ctctaattga atctggaatt gtaacaatga ataaagaagc atgtgaaata tgttaataaa
    15901 catgtgaagt atgttaataa ataggaggga attttatgga ttttagatta acagaagcac
    15961 aattaatgct tcaaagagta gcaaaagaat ttgcagagaa tgaaatagca ccaatagctg
    16021 ctgaaacaga taaaacaggt atttttccaa gggagctttt tagcaaaatg gctaaaatag
    16081 gatttaatgg aattggaaca cctgtagagt atagtggttc tggtggggca gatatagaaa
    16141 aggtaattgt tgttactgag attgccaaaa aatgtgcagc atctgcagca atattatcaa
    16201 tacacacaat atatgctcaa gctattttga aatttggtac tgaggaacaa aagaaaaaat
    16261 accttccaat gatggctgaa ggtgggtgtg taggtgcttt tgccttaacc gaacctaatg
    16321 ctggttcaga tgctgcaaga gctgcaacaa ctgcaattat tgatgaagaa actgatgaat
    16381 atgttttaaa tggaactaag tgttttattt ctggtggagg tcaagcagaa tcattaataa
    16441 tatttgcatt gacagaccca tcaaagggaa taaagggaat gtctgctatc attgtcgaca
    16501 aaggtactcc aggtttttca ataggaaaaa ttgaagaaaa aatgggtatt catggttcag
    16561 aaactgcaga gttaatattt gataactgta gagttccaaa gtctaactta ttaggaaaag
    16621 aaggcaaagg atttaatata gctatgactt gtttagatgg agcaagaatt ggagttggag
    16681 ctcaagctgt aggaattgct gaaggggctt tagaagaaag tatcaaatat tctaaagaaa
    16741 gagtccagtt tggcaagcca atttcagctt tgcaaggtat tcaatggtat atagcagata
    16801 tggcgacaat ggtggaatca gcaaaattat tagtatatta tgctgcagat ttgaaagcta
    16861 gaggagaaaa acacactaaa gaagctgcta tggcaaaata caatgcgtca cgtactgcaa
    16921 gagaggttac aaatttagcc cttcaaatcc atggtggata tggatatatg aaggattatc
    16981 cattagagag aatgtacaga gatgccaaaa taacagaaat ttacgaaggt acttcagaaa
    17041 tacacaaagt tgttatttca agagcagtac taggatagga ggaaaagaat gaatatagtt
    17101 gtttgcttaa agcaagttcc agatactaat gaagttaaaa taaataaaga gacaggtact
    17161 ctaataagag atggtgtacc aagtataatc aatccagatg atagaaatgc tttagaagaa
    17221 gctctaaaaa tgaaagatga actaggagca gtgattaagg ttataagcat gggacctcct
    17281 caagctaaaa gtgctttaaa agaagctttg gcaatgggag ctgatgaagc ttatttaatc
    17341 tcagatagag cttttggtgg ttcagatact tgggctacat ctacaattat tgctgctgct
    17401 atagagaagg ttggaaagta tgatgtaata ttctgcggaa gacaagctat agatggagat
    17461 acagctcagg ttggaccaga ggttgcagaa tttttaggga taccacaagt tacttatgcg
    17521 aaagaagtaa aggttcaaga tgacaaatta ctagtaactc gttatacaga gacaggggat
    17581 tatttaatag aagcaaagat gccagtttta ttaactgcta taaaagagtt aaacaatcca
    17641 agatatccaa gtgtcaaagg aatcttagaa gcttataata atggagatgc aaagattact
    17701 gtattaacac ttgcagattt agatgttgat acaactcaaa ttggattaaa gggttcacct
    17761 acaaacgttt ataagtcttt tgtaccagtt aaagacaagc ataatgaaat aatagaaggt
    17821 ataaataaaa aagaaaaagc tgagaaacta atagaaatac tattcgattt gaaactagta
    17881 taggagtgtt aagatgatga gagcaaaagt taatcaaggt ataaatttaa atgattataa
    17941 tggtgtttgg gtaataggag agcaaaggga aggtaaaatt aatcctgtta cgatagaact
    18001 tattggtgaa ggaagaaagc ttgctgacca attaggaaaa gagcttgcag ttgtgatagc
    18061 tggatatgaa gtagagaaag aagtgaaaga attacttcac tatagtgttg ataaagtata
    18121 ttacatcaat gaccctcttc ttaaagattt cactactgat ggatatgcaa tttcaattgc
    18181 aaatttaatc gaaagaaaaa agccagaggt tgtattggtt ggtgctactt ctataggaag
    18241 agatatagcg cctagaattg caggtaaggt tggaacaggt ctaactgctg actgtacaaa
    18301 acttgaaata gattcaacag ataataagtt attacaaaca agaccagcat ttggtggaaa
    18361 tttaatggcc actattgttt gtccaaaaaa tagaccacag atgtcaacag ttcgtccagg
    18421 agttatggca aaagctgtaa gaaacgaatc agagactgga attttagaag ttgttactcc
    18481 agaacttact gaaaaaatga ttagaactag attagtagag atacttccac aagaaaagaa
    18541 atctgtaaat ctaactgatg ctagaataat agtatcaggg ggaagaggat taaaaagagc
    18601 agaaggattt gagcttataa aagaattggc tgataaattg ggggcagaga ttggtgcttc
    18661 aagagcagca gtagattcag ggtggataga acattctcat caagtgggac aaacaggaac
    18721 tactgttaga ccagaactat acattgcatg tggaatatct ggagcaatac aacatttagc
    18781 aggtatgagt gattctaaat atatagttgc tataaataaa gatgcaaaag cccctatttt
    18841 tagtatatgt gactatggaa tagtaggaga tttatatgaa attatacctg aaatgataga
    18901 atcattaaat aggtaacttt tagaaaacat tgaatatgta gaatgataag tcttataata
    18961 ttgtattgta agacttatta tttttgctga aggttatagt ggggagatga aacaatgaaa
    19021 gagcagtggt ataaggatat atttgcaaga gtattatcta tgactgatga tggttttatt
    19081 gttgtaaata caagtggagt aataatagat attaatgata aatattgtga ctttttagga
    19141 aaagaaagaa aagatataat aggacaaaat atacaaagta ttataccaaa tacaaaaatg
    19201 ctagatgtta tgaaaaataa gtattgtgaa gaaggagcta tacatcatta ttcaggtgga
    19261 aatacaaaag aaaaaagtgt aatagtaagt aggtcttatg tggaaaatga caatggagaa
    19321 gtagttgcag gagtagctca agttaaattt agattgcaat catttgatgt agctaaaaag
    19381 ctaatgtcag aatatatgga gctccaatat tataaagagc agtttaagga taactgtggt
    19441 tttgacaagc tcataggtga aaatagagac ttcatagaac ttaagaaaac aggtgtgaaa
    19501 gcttctaaaa caaactttcc agtactttta acgggtgaaa caggtacagg aaaagaagtc
    19561 tttgctagag caattcacaa taatagcagc aggtcagata aacctatggt tagtataaac
    19621 tgcgctgcaa taccagaaga attattagag tcagagctat ttggttatga cgaaggagct
    19681 tttacaggag caaaaaaggg tggtaaaaaa ggtaagtttt tagttgcaaa taatggtaca
    19741 atctttttag acgagatagg tgacatgcct ttgaccatgc aagcaaaact tttaagagtt
    19801 cttcaggaaa gtgagataga accagtggga ggtcttaaaa ctataaagat agatgtaaga
    19861 gtaatctctg ccacaagaaa gaatttaagt aaaatggttg aagagggatt atttagagaa
    19921 gacctatact atagattaaa tgtaataaac attcatatga tggaattgaa ggacaggcaa
    19981 gatgatattt tattgctagc aaattatata ttaaacaaat taaatgttga atacaaggaa
    20041 ttgaaggttt taagtgataa ggttaaaaat tgttttatta actatacttg gccgggaaat
    20101 ataagagaac tacaaaatgt aataaaaagt gcctatgctg taagtgatga tatggtcatt
    20161 atgatgtgtg accttccatc taagatggat aatatcagca gagtagctca atgtaatgtg
    20221 gatagcaatt gttcaattca tgaaatggtt gagaattatg aaaagagctt aattattgat
    20281 gtgttaagga agtataattg gaaatgttct aaggcagcag aggttatggg aatacacaag
    20341 agtcttttat ataagaaaat aaaaaagtat gaaattgaat taaataatta agataaattg
    20401 aattaaggca atgctaaaaa aacgccctta ttcaaagcga actgtataaa ctgttttatg
    20461 cagttaaaac gtgtaaaagt agatgtgaag tactaatgtc aatcattagt acttttttat
    20521 tatatttata attactatct atatttcagt atcttttgat atctaagatt attatgttta
    20581 atttaggata taacaaaacc ctcttagtta tggacagttt tttatatttt aactgtctac
    20641 taagagggga tagtttagtt taaatttaga gtaaatttta tctagcattt ataaaaaata
    20701 ttttgaatag cttttttgtt atttaatcat aattttctac ttctttatca agttctttgc
    20761 cacaagaatc acacttatat atagcattgt atccgtaatc atattcatca actattttct
    20821 tttgtctaaa tacaccacca cactcacata ggaaaccaat ttttataagt gtaacgataa
    20881 attgtgattt tataaatttt atatcattac atttatcgtt agtgtaaaat gtataaatat
    20941 aagagttatc gcactcatct cttaattcaa atctttcaat atatgtgtta ttttttaatt
    21001 cctcaaattc ttctttagtt aaatatgatt tttgttgcaa ttcatgtaaa cgcttaattt
    21061 ggtccattat ttatatcctc ccaataattt tagtatttta agcttagata aggaatctaa
    21121 gttttattaa atttatccca aggaattatt taaatcatat ttagttttat taattacaac
    21181 atatataaat tacacgaaaa taaaactaat tgtaaaaata atatcctgtg aaaacctaaa
    21241 ctactaaccc cttactaacc cgaaaactaa aaaaagaaaa tatatccaaa tataagtgat
    21301 atataaataa agatgtttca tataaattta actttaaata ttctgacaat aatatatata
    21361 aatatattat agttaaaata taccacatat tttgggtatt gtatactgga attagagtaa
    21421 ttaatttatt ttttaacata caaatgattt tatgaaaaaa aacaagactc aaaaattttt
    21481 atattctttg atagaataaa attgagagta aatttattga attttataaa atttataagg
    21541 ggtgagtcta agatatgcat gaagataaaa atgatatatt tgaatcaata gatactgagg
    21601 attttgataa taaaacagaa gaagaatgtt tagagctagt aagaaaaaat ggattgaatt
    21661 taatatacat aaaaaatcaa acagaagaga tatgtctaga ggctgttaga caaaattgta
    21721 atgcaataaa atgtgttcag aatcagacag aaaaaatatg tatagaagca gttaaacaag
    21781 attggagaat gttagaattt gtggaagagc caacagaaga aatttgcatg ttggctatta
    21841 accaagatgg aacagcatta aaatatatag aaaatcagac agaggagtta tgcttaagag
    21901 ccgttgaaaa aaatggtgcg gctttggagt atgtaaaaga acaaacagaa gaaatatgta
    21961 tagaagcagt tagaaacagc gaatttggat tggcaagagt gaaaaatcaa acagaaaaaa
    22021 tatgtatgga agcagttaag cattgtagct ataatctaaa atgggtagaa aatcaaacag
    22081 aagaaatatg tatggaagca gtaagacaaa atgggcttga tttgaaattt gtaaaaaatc
    22141 aaacagaaac aatctgttta agagcagtaa gacaaaatgg aatggcatta gaatttgtaa
    22201 aggaacagac tgttggtata tgcttaaagg cagtaagaca atatggaatg gcattaaaat
    22261 ttgtaaaaga acaaacagaa gaaatttgta cagaagctat aaaacaagat aaaagagcac
    22321 ttagttttgt aaaaggagat aaggaaaaat acaaggctct atatgataat aatgaaccat
    22381 ttgcaaagag atatgttaga aatgtaatag aaaaagaaaa cagagcattg attaaaaaag
    22441 gagaagaaaa tgcaaaaatc aaaattgcag gaaaacttta tgatagaggc atgtcttttg
    22501 aagatatatc agatatagtt gagattgaaa taagcaagtt aaaagtgagt ttaggtgttt
    22561 tttaaagtaa taaatgtagg tgttttttat gggaaatagt taataaaaaa ataatcgact
    22621 agctctttgt aggacagcaa aattagtgat ataatttgat gtataataaa ttatttttat
    22681 tagataaggg ggaattaaag ttgaagatta cagtattata ccaaacaaga agtggaaaca
    22741 cagaaagggt cgctaagctg atagaagagg gcgctaaaaa ggtggatgga gtagatgtaa
    22801 agcttatgag acttgataat attgatttag attatttaaa tgaaagtaaa gcagtaatat
    22861 ttggaacacc tacttattta gcaaatacaa cttgggaagt aaaaaaatgg tttgatgaag
    22921 attcaaaaaa agttaattta gctggaaaat taggagctgt ttttgctaca tgtgattata
    22981 tatgtggagg tccagatgtt gcgatactta ctatagtagg acatttgatg gttaaaggga
    23041 tgcttgtgta ttctggaggt ggttctttag gaaagccatt tatacattta ggacatgtac
    23101 attcaattga aggtccagag ttacaagatg agaaggctat aatatttggt gaaagaattg
    23161 ctactaaggc aaaagaacta tttgcttaat ttctatatgc atagtaatga ttagagtaaa
    23221 tttagggttt taagggagat ttgaagtaaa agttataaaa taaaaaattt tccataaaat
    23281 tactcaagat gaattataat ataattataa ataaattata ttattaaaat atttaaaatc
    23341 aaacgtcaat agcgagggaa gtttatggta tggcatatag aatatgagat tttttctgca
    23401 attatagtta tttttttaat ggtatacttt tttagaagta aatttatacc tactttacaa
    23461 aacaaaatat attgtgcact tttaatattc tcatttttat ttataatttc aaatatttta
    23521 ggttcatttt gtctaaataa tatagacaaa attccaattt ttattacttt tttattgaat
    23581 cagatatact tattgttatt gccaatacca gcagcacttg tatcatttta tgttatggct
    23641 attatttatc aagatataag gtatatgaaa aaaagattat tatttttatc tataccactt
    23701 ataatttgta taatcttatc tattacaaat ccttttacgc atattctatt tggattgtca
    23761 aaagaaacag gttatatacg aggttgtgga tatgttgcaa cttttgcatc ttttttcttt
    23821 tatgcaatat attcagcatt tttatctttt aaatatagaa aagcaatgca tgaatcaaaa
    23881 atatgggcta ttagaggttt tcttatagtt tcaattgtgg caattatctt acaggcaatt
    23941 tttttacagt atcttcttac aggaactgca tgtgcatgta gtttactttt gacatatctt
    24001 tctatgcaga atcgaggtct tattattgat gaccttactg gtgtactcaa tagacaatct
    24061 tttattcaag agctagattt gaatatcaat actgaacaat caggatttat tattacaatt
    24121 gcgcttgatg attttaagtt tatgaatgaa acttttggaa caaaaaatgg agatattgca
    24181 ctaaaagaag ttggaaaata tttgattcag atacatgatg taaaccatgt atatagaata
    24241 gggggagata tattttctat tgttgtcaat agtcaattag gtatagaacc agatgatatt
    24301 atcagaaaga tagaagaaag atttaaaaag ccttggctag tagaagagat aagttttaat
    24361 ttagcaacta gtattgctgt tgtttattac ccagaaaatg cagaaacgac agaagatgta
    24421 attgttgcta tagatttctc tatacatgaa gcaaaacgtt ctgagtcaag gcgagttgtt
    24481 tatgcagatg cgtctataag tgaaaagata aagaagaaac atgttattaa aaattgttta
    24541 aaagaagcat taaaaaatga tgggtttgaa gtatactacc aacctatatt ttctaatagc
    24601 aggggcaggt ttacttcagc agaggcatta cttaggctag aacacaaaga gttaggtttt
    24661 atacctcctg atgaatttat tccaattgca gaacaaacag gattgattaa ctcaattgga
    24721 ttagtagtct ttgagaaggt atgtagattt atagctagtg atgagtttga aaatttaaaa
    24781 ttggataata tagctgtgaa tctttctgta gtacaatgta tgcaaaaaaa cttagctgat
    24841 gatttacttt atcttatgaa taaatataat gtatcaccaa ctaaatttaa acttgaaatt
    24901 actgagactg tggctatggg ctcttttaat ataattaaag aaacaatgga aagattaatt
    24961 gatttaggtg ttaagtttgc acttgatgat tttggaattg cttattctgg agttactaat
    25021 atgctttctt taccattttc agtaattaag ttagataaga gtttgatttg gtctatgaat
    25081 gaagatagta ggcataaact tacagttgag acgattatag cacttataaa taaattgaat
    25141 atgaaggctg tagcagaagg tgttgaaacc attaagtgtg ctgaagattt aatagctatg
    25201 gagtgtgaat atttgcaagg gtattatttt tctaaaccag ttccagaaga tgtttttaaa
    25261 aatttgttga aagaaaatac ttttacaagt taacaccctt tattttttta attacaactt
    25321 tgttcattga cactgccagc gatatattat aaaattaaca caataataaa tattaaattt
    25381 attatacata ttattaatat taattaatgg agtaaagatt tattatatat tttatttaat
    25441 tatcctaaag tgagaaagga agatgtgaca tgaataaaaa gaaaatatta gtgccaattg
    25501 atgggacaga aagaagtatg cattctttgg aatttataaa aggaatattt aaaaaagatg
    25561 aagttgaagt agaaataatg aatgtaaaag aactagtatt tattgatggc atatccctgg
    25621 ctgaagagat aaaaaattct gaaaatttag gaagaagaat cttggataaa gcagcagaga
    25681 taatgggaga gtatgatgtt aaagtacatt ttacttttgg atatccagga gatgagataa
    25741 ttagaaaagc aaaagaagat aatgtagatt ttatagttat gacaaaatct actaaaaaag
    25801 gtcttacaag aatgataggt tcagtaacag caagtgtagt taaacaagca aaatgtatag
    25861 tgatgattgt accggagtag aattagaaag gtatttgaaa tatgaataaa aagatagtag
    25921 cagtttgtga aagtgaaaga agtttaaatt ctcttaagac agctgcaaat gatttaggat
    25981 ttgatttagt ttatgaaatt caagaaaatg ataatataaa aaatgaactt tctatttatg
    26041 atatagaaga tgcatccata gtgttatttg ttacaagtga ttctatagaa agcatagaga
    26101 agattgatag atttatagat agagaatttt atgaagttga tccaaagtat ataatagagg
    26161 atgcaaaatc tatagtaagt gaaattttaa tagacttaaa ttaatcgtgg gtgtcgcact
    26221 aagaatagag cgacgccttt ttatatattt tacacaagaa ataaagaggt tgtatcaaaa
    26281 tatagataag agtctttttc attgttgaaa tgatgaaaat atatttgatt tcatacaaaa
    26341 aagagtacct cataactaaa aagttcactt tgagacactc catttttata tgttagttgc
    26401 atttaaggta aaatagaagt ttaatcatgt tgaaataact gaattacatc ttctaaatga
    26461 ttgtacattg atgtataacc taagtaagag tctttactat caaatgcaat cattatcttg
    26521 ttatggtggc taatcatatc cttgagagaa tttctatatt taaaaccaaa gtattggtta
    26581 gaagaaactg ttttaggtat taactcataa tattttataa aaattggatt atgagaagct
    26641 aaaattatct ctttgtgaaa ttgcaagtca gcatcataac attcttctat caaaccatga
    26701 gaaaccttat ccaattcctc tattgacttt aggtaagtat attttatttt ttgaagttct
    26761 ttagatgtag catttaaaat tgctagttta gtagtttcgc tttcgataag ttgtcttgct
    26821 cttaaagatt ctacataaaa ttcttgtatg ttatgatttt tatagatttc accatcatca
    26881 aataggtcaa cttttttaaa tccttcaact ataggacttg aataaggcac agctctaagt
    26941 attccttcct tacagagtat atttatggca tttcttacag gtattctact tatgttaaat
    27001 aggtcagcaa tctttctctc acttggtaat ttactattaa ttttaaaagt tccatttaga
    27061 atgtttaatt taatataatc aataattttt tcagtggcat ttaagctttt cataaaaact
    27121 cctaaacata taattttttt atgattgtat tatatcatag ttaagagtta aatctacttt
    27181 tttataatat gtataagaca ggatagaggc caaaatagct ataatagcca agataaagta
    27241 tccatattgt attgaagtat attccgatat tttaccaact atcattggaa atacagacat
    27301 tcctatagca taaattgctt gaaaaaaacc cattgcagta gatttctgat gttttggtac
    27361 atttttcata gactcgctag taaggtaaga aaggagtata cctgtagata tacctggtag
    27421 tacttgtagt acaaatatta tattaatatt ttttattgtt ggaactaaaa agcaataggc
    27481 tgaaactaaa ataaaaacta aaggaatcca aaattttggt ccatacttag tacaaaattt
    27541 agtagaagca aagccagaag aagcaacagc acacaccatg taaaatatag aagatagacc
    27601 tatgagggta gaactagaac ctaagttttt aagaatttga gtagtaaatg acattgttgt
    27661 agacatttga ataccttgtt gaattaatgc cataaaggaa aaaaatacaa gtcttttatc
    27721 cttgcagata gataaatatt ttttaattga tattttttca ggtatttttt tagaattatg
    27781 attttttata tttaaggcta gaatcgttcc aataactgaa gacaatacac tcatgataca
    27841 taaggctgtc attcctaatt tttcataaaa taaagtactc ataacaaatc caatcaacat
    27901 tcctagattg tttaccaata ttatcgtact tgtagctttt tgttgatgtt ctgtagaaaa
    27961 aaagttggta tagtgaacca tgtaagaaat ccaagttgat gatgatattc cagataaaat
    28021 gttagctata agaaaaccat tgcctccagg agaaaagact cttataagag atgctaaacc
    28081 agcaaagaaa attccagaca gtataaatat tttatgttta tttatggaat cagcaaatac
    28141 tccaacagga agccttaata aacattgtga tattccatat gcacctatta ttataccaat
    28201 catatttgta gatacattac atgcacttaa atatggattt tggaaaggta tatatacata
    28261 ttgtgaaaac cagaaaaatg taacaataga aagtaaaagt aatttttgat tagatatttt
    28321 tttcatatta tcctccatgt ggtattttta taatgcaaat aatataccat ttagattttt
    28381 taatcaagta ctttctctat taaaaaaaat ttaaatatat aattattatt tacaaaaaaa
    28441 ttatacttta tagttgaatt aagaaaacgc ttatgatact ataaagttaa caaatttgaa
    28501 tatataacag tagacgtgtt actggtaatg caggcaagat cgaatcaatt tttatagatt
    28561 atctatagag tactatgggt ataatctatt tatttggttc gatttttttg tttaattggc
    28621 cataaaacaa aaagcctata gttgaaaatg gaggattttt tatggattat aaaaatatag
    28681 ctcaagaaat tctactaaat gtaggaggaa aagagaatgt aaatgaagtg actcattgta
    28741 tgacaagact tagatttaaa gtcaaaagcg cttctaaagt gaataaggat aaattatcta
    28801 aaactgaagg agtaataact gttgttgaga gtatgggtca aatacaagtt gtaattggaa
    28861 ataaagtaaa aaaagtatat gatgaggtca taaaaattgt acctcaatca aataacatag
    28921 gtaaaaagaa tgaaagtcaa gaaaagcaag gtataattaa ttctatatta tcagcagtag
    28981 ctggtatatt tacacctaca atacctgcta tagcaggggt tggtatgatt aaaggtattt
    29041 tatcagttct tgctatgtat tatatgaata aaaatggagt agatataaaa gaaacgcaaa
    29101 gctatattat cttaaatgct atggctgatt ctatattcta ttttatgcct attatactag
    29161 gatatactgc ggctaaagta tttaatgcaa ataaaataat atctatggta ttaggagcta
    29221 cactttgtta tccaactttt actgctttga tggcaggtga agaatctgtt agatttttag
    29281 gattggcagt aacaaaagct acgtatacat catctgttat tccaataatt attgctatat
    29341 gggctctttc atacgttgaa aaagtattag aaaagtatat acctgaggta attaaaataa
    29401 taatggttcc aactttatca ttggtagtaa tgttacctgc aacattattt ttatttggac
    29461 caatcggaat ttatatagga aatgtcataa acttttctta taagtatatt tatgaattaa
    29521 gcccagcatt gtgtggagca tttgtaggtg ggttatggtg tgtgttagtt atatttggtg
    29581 ctcatagagc attattacca ataggtataa gtgatgttgc tcaaacagga agacaaaatt
    29641 tattggcgtt tgcaggagct gcaaactttt cacaagcagg ggctgctcta ggagtattct
    29701 ttaaaactaa aaatcaagga ctaaaaacaa tttctatgtc tgcaactata acagctttat
    29761 ttggtataac tgaacctgca atatacggtg ccaatcttag attaaaaaaa cctatggtat
    29821 gtgccgtaat atgtggtgca tttggaggtg cgataatggg aatgggtgga gcttatggta
    29881 atgcatttgc aaaccaaggt gttttaacta tacctgtata tgctgaagca ggtgcattag
    29941 gtttcttaag ttatctaggt ggttgtgcta tagcattttt tggttcagct atttcaactt
    30001 atctagttgg atttgaagat ttggaagagt cttctaataa ggaaaattca tctataaaag
    30061 tagaaacgaa ggatggtgtt attgatataa cttcacctgt agagggcgaa tgtatagaat
    30121 tgtcagaagt aaaggatgat gtatttgctt caaaagctat gggagaaggt atagctgtgt
    30181 tacctacaaa aggagtaata acagccccta ctgattgtga agtagcttct ttatttccaa
    30241 cattacatgc aataggatta aaattagata atggagcaga aatgttaata catgttggaa
    30301 tcaatacagt agagttaaat ggtaaatact ttacaaaaca tgttaatcaa ggagatttag
    30361 tgaaaaaagg tgataaactt atatcctttg atatagataa aataaaaaag gctggttatg
    30421 atgtaactac gccagttata gtaaacaata catttgatta aaaaatagct acgcacactc
    30481 gtgactttag tcatgagtta gtagctaaaa tagtcagcat atagggaaac ctatatgtag
    30541 agataggata gaacctatcc aatactactt gaattgctgg aaacccctaa agctaaccaa
    30601 actaaaacat aaggatgaaa taaacctaag tgtgaaagtt acgaaagtag aaaaaattgg
    30661 ttagatggta taaggttaaa tcctaagtac tttaaaatgg gcaatcagca ggtaaactcc
    30721 gaataggaga aacttcaacg actattcctc ttgagggaag tacactacaa gttatcggta
    30781 gtggaagtgg gtagacctta acggataatg ccgaaggata agatatagtc tgtgcttgca
    30841 tgaaagtgta agaagttcat aagagaactg cataggtagt agcgaactta tgtgaacgac
    30901 aacctcaaaa atgataaaag ttaagactta cggttcttaa atatgttatt taaaattaaa
    30961 tataaaacct tctaaataat tgttttaaat aattacattt gttaacatta ttataggtga
    31021 ttaataaatt gttcaccaca gcattatata atatattaaa tgtgataaaa tcattaaaaa
    31081 gtgtatcaaa aaacaattaa agtgaggtgg tagtagtgga aaaagcttat aagtttagaa
    31141 tgtatccaaa taaaaagcaa caagagttaa ttaacaaaac ttttggatgt tgtagatttg
    31201 tatataataa gtatcttgct aaaagaatag atgtttataa aaacaataaa gaaactttta
    31261 cttataaaca gtgtagttct gatttgacca atttaaaa
//
LOCUS       DAGSHT010000025        30987 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGSHT010000025 DAGSHT010000000
VERSION     DAGSHT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.026"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..169
                     /locus_tag="MJB72_001075"
     CDS             <1..169
                     /locus_tag="MJB72_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262200.1"
                     /translation="ELHEGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNEL
                     SKKLEIEFSDL"
     misc_binding    252..492
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    558..793
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            878..2095
                     /locus_tag="MJB72_001076"
     CDS             878..2095
                     /locus_tag="MJB72_001076"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBY8262201.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            2212..2694
                     /gene="cdeM"
                     /locus_tag="MJB72_001077"
     CDS             2212..2694
                     /gene="cdeM"
                     /locus_tag="MJB72_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBY8262202.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            2958..4223
                     /gene="hisD"
                     /locus_tag="MJB72_001078"
     CDS             2958..4223
                     /gene="hisD"
                     /locus_tag="MJB72_001078"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBY8262203.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(4426..5178)
                     /locus_tag="MJB72_001079"
     CDS             complement(4426..5178)
                     /locus_tag="MJB72_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8262204.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(5296..5565)
                     /locus_tag="MJB72_001080"
     CDS             complement(5296..5565)
                     /locus_tag="MJB72_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262205.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            6040..7134
                     /locus_tag="MJB72_001081"
     CDS             6040..7134
                     /locus_tag="MJB72_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY8262206.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            7136..8086
                     /locus_tag="MJB72_001082"
     CDS             7136..8086
                     /locus_tag="MJB72_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBY8262207.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            8079..9263
                     /locus_tag="MJB72_001083"
     CDS             8079..9263
                     /locus_tag="MJB72_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262208.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            9276..10772
                     /locus_tag="MJB72_001084"
     CDS             9276..10772
                     /locus_tag="MJB72_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBY8262209.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            10795..11745
                     /locus_tag="MJB72_001085"
     CDS             10795..11745
                     /locus_tag="MJB72_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262210.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            11766..12728
                     /locus_tag="MJB72_001086"
     CDS             11766..12728
                     /locus_tag="MJB72_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY8262211.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            13100..13732
                     /locus_tag="MJB72_001087"
     CDS             13100..13732
                     /locus_tag="MJB72_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY8262212.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDSLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            14143..14235
                     /gene="kdpF"
                     /locus_tag="MJB72_001088"
     CDS             14143..14235
                     /gene="kdpF"
                     /locus_tag="MJB72_001088"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBY8262213.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            14276..15958
                     /gene="kdpA"
                     /locus_tag="MJB72_001089"
     CDS             14276..15958
                     /gene="kdpA"
                     /locus_tag="MJB72_001089"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBY8262214.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            15983..18040
                     /gene="kdpB"
                     /locus_tag="MJB72_001090"
     CDS             15983..18040
                     /gene="kdpB"
                     /locus_tag="MJB72_001090"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBY8262215.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            18059..18685
                     /locus_tag="MJB72_001091"
     CDS             18059..18685
                     /locus_tag="MJB72_001091"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBY8262216.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDVRFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            18922..19830
                     /gene="cysK"
                     /locus_tag="MJB72_001092"
     CDS             18922..19830
                     /gene="cysK"
                     /locus_tag="MJB72_001092"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBY8262217.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            19857..20447
                     /gene="cysE"
                     /locus_tag="MJB72_001093"
     CDS             19857..20447
                     /gene="cysE"
                     /locus_tag="MJB72_001093"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBY8262218.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            20607..20795
                     /locus_tag="MJB72_001094"
     CDS             20607..20795
                     /locus_tag="MJB72_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBY8262219.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(20849..21718)
                     /locus_tag="MJB72_001095"
     CDS             complement(20849..21718)
                     /locus_tag="MJB72_001095"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBY8262220.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(21915..23159)
                     /locus_tag="MJB72_001096"
     CDS             complement(21915..23159)
                     /locus_tag="MJB72_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262221.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFNEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            23450..23761
                     /locus_tag="MJB72_001097"
     CDS             23450..23761
                     /locus_tag="MJB72_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY8262222.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      23854..23946
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24046..24840
                     /gene="thiD"
                     /locus_tag="MJB72_001098"
     CDS             24046..24840
                     /gene="thiD"
                     /locus_tag="MJB72_001098"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBY8262223.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            24864..25652
                     /gene="thiM"
                     /locus_tag="MJB72_001099"
     CDS             24864..25652
                     /gene="thiM"
                     /locus_tag="MJB72_001099"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBY8262224.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVKGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            25675..26307
                     /gene="thiE"
                     /locus_tag="MJB72_001100"
     CDS             25675..26307
                     /gene="thiE"
                     /locus_tag="MJB72_001100"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBY8262225.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSVSFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            26446..26748
                     /locus_tag="MJB72_001101"
     CDS             26446..26748
                     /locus_tag="MJB72_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8262226.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(26814..27563)
                     /locus_tag="MJB72_001102"
     CDS             complement(26814..27563)
                     /locus_tag="MJB72_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262227.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     VDILPFILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(27557..28519)
                     /locus_tag="MJB72_001103"
     CDS             complement(27557..28519)
                     /locus_tag="MJB72_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262228.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNSSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(28523..28981)
                     /locus_tag="MJB72_001104"
     CDS             complement(28523..28981)
                     /locus_tag="MJB72_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY8262229.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            29337..29693
                     /locus_tag="MJB72_001105"
     CDS             29337..29693
                     /locus_tag="MJB72_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262230.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            29765..30481
                     /locus_tag="MJB72_001106"
     CDS             29765..30481
                     /locus_tag="MJB72_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262231.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            30474..>30987
                     /locus_tag="MJB72_001107"
     CDS             30474..>30987
                     /locus_tag="MJB72_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262232.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIP"
BASE COUNT        11888 a         3446 c         5461 g        10192 t
ORIGIN      
        1 tgagttgcat gagggaagta tatctttaaa aagtcaactt ggtattggaa gtgaatttat
       61 agtttcattg cctgttagaa gtaaaaataa tattgaaaaa tataatcata aaagagaaat
      121 cagcaatgaa ttaagtaaaa aattagaaat agagttctcg gatttataag tttaaatata
      181 aaaaataaaa ttttaaaaaa gtattgacaa ttgatttaga agggtttaaa ataaattaca
      241 acataataaa taaaaaggcg atgaatagga agagtaaatc taattcgatt accagagaga
      301 agagtccgtt ggctggaaga ctttttaaat tagagggatt gaaaactacc taggagctac
      361 tttctgaagt catctgcatt agtagatttt agtaagaata gtcgttagct gcgttaaagt
      421 gtaaagtgag ttttcatgac atatgaaaac taaattaggt ggaaccgcgg gaataatact
      481 ctcgtcctta tgttataagg atgagagttt tttgttattt aaaaatataa acagtaatca
      541 taataattat agaaaatata aaagcaatga ataggaagag taaatttaat tcgactatca
      601 gagaggaaag ttcacaggct gaaagacttt ctaagtttga gaaattgaaa actacctagg
      661 agctgtggtc tgaagttact ttaaaaagtt ttagtaagac taaccgttaa ctgcgttaaa
      721 gtgtaaagtg gattttatca ataaatgata aaatcaaatt gggtggaacc gcgagtatag
      781 actcgtccct ttatataggg atgagtctat ttttattgca ataaaagatt gaaaataaaa
      841 aaaaatcgga aaattataaa tttaggagga aaataaaatg gagaataaag tgaaaattgg
      901 agtactagga tatggagttg taggaagtgg acttatagat ataatagata ataataaaga
      961 aaaaagaaat atagaaatag ttggaatttt agttaataat ttggaaaaac ataaggataa
     1021 aaaatattct aatataataa ctaataatat agatgatata tttaataaag atatagatat
     1081 acttgttgaa gtcatgggtg gtttagaacc atcattatct tatataaaaa aagcattaaa
     1141 caataaaatt catgttgtta cagcaaataa agatttgtta gctgaatgtg gagatgagct
     1201 tgcaaaatta gcaagtgaaa ataaagtaag tattaaattt gaagcttcag ttgcaggagg
     1261 tataccagta ttaaaaccaa taatagaatc actagaagga aataatatag atagtataaa
     1321 tgcaatttta aatggaacta ctaattttat attatctaaa atgtatgatg agaatctatc
     1381 ttatgatatg gcattaagac aagcacaaga attaggtttt gctgaagcaa atccagagtc
     1441 agatgtatta gggtatgatg ctgctagaaa actatcaata ttatcaactc tagcttatga
     1501 caatagagta tattggaaag atgtgtattt agaaggaatt acagatatag atgaaaaaga
     1561 tattgaatat gcaaaaaaat taaattgtaa aataaaattg attggtcaaa gtaagtatga
     1621 aaatgataaa gtaagtgctt ttgttagacc tgttttagta gaaaaagata atatccttgc
     1681 tagaatagac aatgagttca atgctgtcat agtgaatgga gattcagtag gagaagtttc
     1741 ttttgtaggt aaaggagctg gaagtttagc aacaggaagt gcggtttatt cagatgttat
     1801 agatataata gataatagag tttcaagtat agattctttt actaaagata aaatacaagt
     1861 taataaaata gttagagaaa agtgtggagc acttcttaga tttaaaaaat gtaataaaga
     1921 tgaaattcta aatatagttg agaattgttt agtaaaattc gatattttaa atgatgatga
     1981 tgaacttgct attatggttt atgctgattc tgaatatgaa ataaacaaca gtttatgtct
     2041 aataaaagat aaaggatact gcgaaaaaat gaataaaatg ttgaaaataa gttaaaaaag
     2101 aaaaaatata agtttatatg taacatattt tgacgatttt aatactatat agtatgagat
     2161 gaaacaaaac atctcataaa aaaattaaaa ttaaagggag agatatgtta tatggaaaat
     2221 aaaaaatgtt attcagaaga ttggtatgaa agaggagaat ctacagctaa atggttccaa
     2281 aatgatagag aagaatatga aagagaagca tatgatgaag atagagaaag aagaggttca
     2341 aactgtggat gttcagattc aggagaaaat agacctagaa actgtgaaag atttagaaga
     2401 gaagctgaga taagagaaag agaagcaaga gaagcattct gtgaatcttc agagaaaaag
     2461 aaagaggcat tagcatatga atgtgaagct agaaaattat gggaagaagc agaaaaatac
     2521 tgggatgaat attcaaaata caactataaa ggaatcgaat atttagcaga agctgctaga
     2581 ttatttgatg aaggtatgga atgtgaagct agaagaaatg gaaataatgg aggaaacaat
     2641 aataattgtt gccataaatg ccataaatgt aattgtaact gctgtagaaa ataaaaaaat
     2701 taattgacaa atcaatagtt aaaatatatc atattatgta taataaagag aaaattcaga
     2761 aaaaaataat gattagaaga aatataagta tatttattag acaaatagtg ctcagttagt
     2821 gaggtgagta caatatgatt aaactcccat cctctagctt ttgatagagg gtgggagttt
     2881 ttatttattt atatatctta aatgaggata atattgtaaa ataaatgagt atgagattgt
     2941 aaaattagga ggtaaaaatg aaaagtatta aaacatcaag tatgaaagaa aaggtagatg
     3001 aaaagatatt aagagagcaa gtaagttcta taatagaaga tataagaaat aataaggatg
     3061 ttgcacttaa aaaatataat gagaagtttg atagaaatac aagagatgaa tttagaatta
     3121 ctaaggaaga aataaaggaa gcttataagc atgtagatga cgaatttata aataacttaa
     3181 agattgctgc caaaaatata agagaatttg ctaaagctca aaaactaagt tttgaaaacc
     3241 cttttgaaaa agaaatttat ccaggtgtaa tcttaggtca aactaatatt ccaatagagt
     3301 catgtcttgc atatgttcca ggaggtcagt atccattgtt ttcaacagct cttatgctta
     3361 taatacctgc aaaagtagca ggagtaaaaa gaatagtggc atgttcacca actatgaaaa
     3421 atactgaaaa gataaatcct aagacattgg tagctatgga tatagcaggt gctgatgaaa
     3481 tatatgcaac tggaggagtt caagctatag cagcatttac atatggaaca gaaaagatta
     3541 atccagttga cataattgta ggaccaggaa ataagtttgt aactgaagct aaaagacaat
     3601 gttatggaca agtagggatt gattttgtcg ctgggccaag tgaagttctt ataatagcgg
     3661 atgaaacttc aaatccagtt tatattgcag ctgatttatt agcacaatgt gagcatgatt
     3721 taaatgcaag aggaatatta ttgacaaata gcttggaagt agcacaaaaa gttgaaaaaa
     3781 atatagaaag tatgctaaaa gatttgccaa caaaggacat agcatatagt tcatgggaaa
     3841 ataatggaga gataatattg gttgatgata tggaagaagc cattaaaata agtaattttt
     3901 atgcaccaga acatttagaa gttgcagtaa atgagtataa tgatatttgt gatagattaa
     3961 ctaactatgg ttctttattt attggaaatc tttctgctga ggtgtttggt gactatgttt
     4021 caggtaccaa tcatacatta ccaacactta aagcatcaag atatactggt ggtgtttggg
     4081 ttggaacttt tatcaaaact tgtacaaagc agatatttaa tgaaaaagct atacaatcat
     4141 tagcacctgt agctgaaaag ctggcaaaag aagagggatt atatgcacat gcaaaggctg
     4201 ctgaggttag gtttaaaaag tagtaaaaaa ttttattttg gcagtcgaga cttagtatta
     4261 gactgagata aggaaaacgc tgagaacctg aataaatact atattttgtg ggtctcggcg
     4321 cttcttttat atctgtatat aatagaataa tactatctgt gtgtttaaat actaacttaa
     4381 tatttctttt ctaaataatg tttatgaaat agttaatatt tttagttaat acttgaaaaa
     4441 aacttttgct aactgtgcaa attcatctgc atttgtcaat atagcagggt gtcctccctg
     4501 actaaaaatg taaatacttg cttttgaaat aaaagttgcc atttgcttgt actcttcttc
     4561 aagattatga cgacacattt cgtcttcctt acttcctgta aataaaattg gcatttctaa
     4621 tttacacaat tctttatgaa acaaagggcg tttctcgttt gcacattgca atagagcttt
     4681 tgtatcaaga ttaacaacat tctcccaatc ttttccatga caccattcat agaattttcg
     4741 tgcttgaata tcttgctttg atttttccct tccagtgatt agattatcta tgaagttatc
     4801 attaagagta cgtccatcaa agctatctgc tataattgct tcaactaaat cgggacgttc
     4861 taatccagta ttaacagctg cccatgctcc tccacttgtt ccaattaagc caactttacc
     4921 acaattcaaa tgttcaatca gtgctattgt ctgtaaggct tcatcatgcc atatgtcagg
     4981 agaaaattga gaaactctat ccgactgtcc attacctaaa aaatcaatta agatacatct
     5041 aaaattttct gcatataatg gcacaagtgc tttaaacata ttagaagaac cagtattacc
     5101 atgcaggaaa attatcggtt taccttgtcc atattcttta taaaaacaag aattaccttg
     5161 atactgaaaa tatgacatat attttcctcc tttttatttg ttaagttaat tataacaata
     5221 taggtatgaa tgaaaaacct catgttcttt cttagaataa aaatgtgcca cgcataaaac
     5281 gtgacacatt tctaattata aattttgctg tttaagtatt ctttgtatgc ttttctctgt
     5341 caaaaagtat ttctctgata actgcttgat actcattcca ttttggaaat ttatacagat
     5401 ttgagaatta cgagaagcta agacttgttt ggtgtttgtg ttttcacccc aatgcttttt
     5461 gtgctcttgc tttttgggaa tataaatcac tttaccatct atatattctt gcacttgctc
     5521 aatcagttct ttaggcagta agtcaatcga attttgatag ctcattttgc acctcctaaa
     5581 taattattct ttaggattag caaaggctac tgccactcac aattaaaaat aaaatttttt
     5641 attatgtaca atagcctttg ctatgcacaa gttccaattc ccataaacta aatactcctt
     5701 tctggtttga aaaattcatc tgaacattga tttcgctttt aagatagagt tcaatgacaa
     5761 atttacattt ttattatata tattcagata gataattgca actgtctttg tatgaggata
     5821 ttttattata gtcaatacta taatatgtat ttatatcatc aaactaatat gaacagttga
     5881 aaggaatatg aacaataaaa cttagtcagg aagaataatt atgaagcaga caatagagaa
     5941 atttgatttt aaagttttat tgaaaattta ttgacaaatt taacttgtaa atattatact
     6001 aaacttaata aataaagttt ataaagtaag gtgttgtcta tgaaaaatat agttggaaaa
     6061 ccgcaaacga taaaaaaagt aaatgaagat ttaataaaaa acattataaa aaatgaaggt
     6121 cctataacaa aaccagaaat ttcaagcatt actgaattga gtttagctac agttaataag
     6181 attgtagagc aattagcttt gaaaaatgaa gttaaagtaa gtggactaag tgaatctact
     6241 ggaggaagaa gagctcaatt atttgaaata aatgcaaact tagaacacat aatagcttta
     6301 tattattata gaaattgttg tataggggta gttgccaatt tattaggtga aataatttat
     6361 caagaagaat ttaatataag aatggatacg tatgaaaacg ttaaccaaga tacttttttt
     6421 gttatagata ctctaataaa aaaggaaaaa ggaaacaata taagggctat tggaattggt
     6481 gtcccaggag ttgtaaaaaa aggtgtagtt aaaaatatac caactataaa aagttgggaa
     6541 ggtataaatt tagaaaattt aataaagagt aaatacaata ctaaagtgtt tatagaaaat
     6601 gatacaaata ttactactat tgggatttat caaaaacagt ataaagacaa atataaaaat
     6661 atggctctta tgtatttaga agatggtatt ggttcaggta tagttataaa tggtgaattg
     6721 tatttaggtt ctacaaattt tgctggagaa ttaagttatc taaatataga atttggtaag
     6781 tataactcta acaacttaaa tcttgaagat tatattatga gtttaagaaa aagatattta
     6841 gaaagtaaag ataataaatt taagaaagaa attttatcta taatatcaaa ctttataagg
     6901 actcttgtat gtgtcctaaa tccacaagct atgattatac agtgtagtat attaaccaag
     6961 agtgacatgg agtttataga aaaagatgta agcaataatg ttggagaaga aaattttcct
     7021 aaattaataa aagtagactc attgagagaa ggaagtataa atggagtaat tagtatgtgt
     7081 ttaaaagaaa ctgactatca atattcctta tctaataaaa aaggaggata ttaatgtgaa
     7141 atatttggtt tcaatagatg gagggggaac aaaaactaaa ttttgtgtaa gtgacttaga
     7201 tggaaacata cttaaagaac ataccactgg ttcaactaat tataagtcag ttggaattaa
     7261 aaaaacatat gaaaatatta ataatggatt taaaaaaata ttaaaagatt tatatatcga
     7321 ttatgacgat attgagtaca ctgtatttgg tatatctggt tgtgattctc ctaatgatta
     7381 taagattata atggatgaaa tattaaaaat tgggattaac aaagaaaaaa tatatctggc
     7441 aaatgatgca gttttagctt tttatgcaca agctgattcc cctggcttag taattgtggc
     7501 tgggacaggt tcaattatac tgggaattaa agaagatgga gaaatatata gagttggtgg
     7561 atgggggtat aattttagtg atttgggttc tggatatgat atagggagaa aattattaaa
     7621 gaaagtattg ctttattgtg atgaatgtca tgagtatagt gatttattta gttgtgtctt
     7681 ggacttcttt ggagcaaata gttttgaaca acttgcatat atgattactg atataaatga
     7741 taatgttgaa atagcaaact tagcttcatt agtgattgat tgtgccgaac aaggtgataa
     7801 attggctatt gaaatattaa gagaatcatc aactgaacta tctaaattag ctcaagtaat
     7861 attgagaaaa atatctaatc caatgaaaat ggataaaagc gaaataaata tagttttgtc
     7921 tggtggtaca ttaaataaaa ctgtatatgc tcaaatgtta atgaataatt taaaaacaga
     7981 aaatacagat aaaaatttaa aatttatatc tcaagaaaat cagccagtat atggggggat
     8041 aagacttgct atggcattag ctaataggag ggtcaagcat ggatagaaaa aaggtaatag
     8101 tacaaatttt tactggaggt tttaaggcta aagaaataaa gtttaatgat ataaaatgta
     8161 aactactaga attattggac tgtattgata tagaaaaggt tatcatggga tggagtgtag
     8221 atgaagaatt atatgtagaa actaaaaaac tattaaataa atataatgtt gaactatatc
     8281 tttggttgcc atgtttttct gaaataggat tacttgaaaa aagtaattta ctaattgatt
     8341 atatggggaa ggaagtcaag ggctatgctt tacaagagga tgaaaatttt gagttttatt
     8401 gcccaaataa taaggaaaat ataaaaaata taaagaaaat atatagtact tattttagtg
     8461 atattaaatt tgatggaatt tttttagata aaataaggta tggttctttt tcgaataaac
     8521 taaatggtgt ttttaattgt ttttgtaaag attgtttaaa tatatataaa aaaaataata
     8581 ttgataaaga gtcgttaatg agagaaatga ataatgtgtc taagggagtt atagataatg
     8641 aagatactcc atttggtata acagaataca ttaatggaaa atacaaattt aaaaataaaa
     8701 tatgggaaga tttttttgaa acaaaaaatc aaaatatata caaggcatta ttagagataa
     8761 gtagttattt taaaagtaag ggattgaaaa taggtataga tgctttttct ccttttattt
     8821 cttactttac aggtcaagat ttagaaaaat tacaagaagt agcagatttt ataaaaccaa
     8881 tgatgtatag aactacaaaa gctccagctg gtttaccatt tgaatttgaa aggtttattg
     8941 atgaatccat acataagaat aaagagattg gaaaaagaaa tttcttagat attttaggaa
     9001 ttaaggagta tgaaggagat gtatatccta tagaatttgt actagaggaa atcgatttta
     9061 tgacaagaaa tcttaataaa gatatatatg taggagttga aataaataaa aaggaaatta
     9121 tatctcctgt tacacctaag tacataagtg aaaatatttc aaatataaat aagacaacag
     9181 taaaaggata tgttttatct tgggatttgt tgtcagcacc aaaagaaaat attgatgaaa
     9241 ttataaaatg gtttcggaat tagtgaggtg ataaagtgag taagatattg gaaatacaac
     9301 aaatatataa aagttttgga aaggtagatg ttttaaaagg gataaatttt gagttagaaa
     9361 agggagaagt aagagctctt ttgggagcta atggagcagg aaaatctaca cttattaaaa
     9421 ttttaggagg agttcattct caaactaaag gtaaagtaat tttgactggt aatgaaataa
     9481 tgtttaaaaa tccacatcat tctaaagaaa atggtattag tataatttat caagaattaa
     9541 gcttgatacc aacgcttaca gtacttgaaa atatttttct tggtagagaa attttaaaag
     9601 gaagtgtgtt tttaaacaag aaagacatgc ttgaggaata tatgaaaatt tgtgaggatt
     9661 ttgattttaa tatagaccca catatagtag tttctaattt aagtgtagca aaccaacaaa
     9721 tggttgagat aatgaaagct gtttcatgta attctgaaat tatcataatg gatgaaccta
     9781 caacatcact tactaataat gaaaaagatg ggctatttaa aataataagt agactaaagt
     9841 ctaaaggtaa aacaatagtc tatatttctc acatattaga ggaagttttt aaagtatgtg
     9901 atagtgcaag tataatgaaa aatggaatta tgattggaac ttatgatgta gaggatttaa
     9961 ctaaattaaa gataactcaa cttatgacag gaaatgataa tcacaaactt attgagaaga
    10021 aggattatac atttgcaaaa tatgaagagc caccagtact agaagtaaag aatataagct
    10081 ggtctaacaa aataaaaaat gtttcattta aagtttatag aggcgaagtt gtaggtcttg
    10141 caggtcttgt aggttcaaga agaactgaaa ttataaattt gatatttggt atagataata
    10201 aagatagtgg agatattttt atcaatggta aaaaagtaga cataacttct cctaaggttg
    10261 ctataaaaaa caaaatagga ttgattcctg aagatagaaa aaatctagga ttaattttag
    10321 aacaagctat atacacaaac tcatcatcag tacaaattga taaattcaag aaatttttgt
    10381 ttatagatag aaataaagaa attgagtttt ctaaaaaagg tgttaaggaa ttaggaataa
    10441 aagttagtga tgtaaagcaa gaagtaaaag agcttagtgg tggaaatcaa caaaaagtag
    10501 tagtttctaa gtggttagag aaagatttag atttaataat atatgatgag ccgacaaagg
    10561 gaatagatat tgctgcaaaa gaagatattt tcaatacaat caaagaattt tcaagtaaag
    10621 gaataggagt aatatttata tcttctgatt tagaagaagt aataagagta agtgatagaa
    10681 ttttagtaac tagagaaggt tctattgtag atgatttaaa aaatgagaat atacaggttc
    10741 aagatataat gaacagtata tttaatgtat aaaaaatgaa aatggaggac tgagatgaag
    10801 aaagcttttg agaagttaaa tttaagagat tatggagtat tattaggttt tttagcttta
    10861 tgtatagcta taagtattgc aactccatca tttttaggta aacaaaatat acttaatctt
    10921 ttacgacaat cttctataat tggaataatt tcagcaggta tgacttttgt tataatatct
    10981 ggaaactttg atatatcagt tggagcagta gcagctcttg caggtgctat aacaatgaaa
    11041 tttgcgactg gtggaactaa tttgtttgca tccatgttct taggtgtagc gatttgtgca
    11101 gtaataggat taatcaatgg tatagtagtt gcaaagataa atgtaccatc attaatagcc
    11161 actatggcta tggtcactat tgtaagagga ttgctgttga tgcttacagg aggatatcca
    11221 attactgaaa atataccaat acttgattac attggaaatg gatatttgtt taatatacca
    11281 ataccagtag taattttctt tatagtggtg ttagcttcgt ttattgtact taataaaact
    11341 aagtttggta gatatgttta ttctgtagga gggaatcaag aagcaagtaa acttaatggt
    11401 ataaatgtag attctcacaa agtgaaagtg tttattatta atgctgtact tgcaggaata
    11461 gctggaatcg tactaacagg aagacttggg actgcaactg cgattgcagg tgaaggatat
    11521 gatatggatg caattgcatc tgtagtaata ggaggtacat cagtggcagg aggttctggt
    11581 tctgtactta aaactgttat aggagtatta cttatgagtg ttataaacaa cagttttaat
    11641 cttcttggaa tagatgtata tttccagtac atatttaaag gattaataat tttagcagca
    11701 gttggatttg attcttatag caagaaaaaa cttgcttcaa gataaaaaat aagggggatt
    11761 tttttatgaa aaaattaatg aaacatttag cattgttgct atcacttgtt atgatatttg
    11821 gtttagttgg ttgttcaaat ggtggtgaca aagaaaaaaa tggagaaaag aaaatagcag
    11881 tattactacc gggttcaaca ggttactttg tagcaactaa acaaggtata gatgcaaaat
    11941 ctaaagaatt aggtgtaagc gtggaatatg cagatgctca atgggatgct agtaaacaat
    12001 tatcacaagc tgaagatttt atggctaaag gggtagacat gattgcatta tgtggagttg
    12061 attctgctgt aagtgagaga atagttaaag ctgctaatga ttcagacgtt cctatagtag
    12121 catttacaaa ttctatagga agtaacccaa ctggagaatt taaaggttta gttacatata
    12181 taggacaaaa tgaggaagaa acaggagcat taactggaaa aattgctaaa aacttattag
    12241 gagaaactgg tgggaaagct gtattaatag aaggagtacc aggaactact ccacaagtat
    12301 taagaaaaaa aggtcttgaa aaagaattaa aagatagtaa tatagaaata gtttataatc
    12361 aaacttcaaa ttgggagaaa gagcaagctt taaaggtaac agaagattta attcaaaaga
    12421 aaactgattt taatgttata atatgtcaag atgataattc tgctacaggt gcaggtcaag
    12481 ctttaaaaga tgctggtctt aaggataaag ttaaagtcat aggtttgggt ggaagtaaag
    12541 atggattaaa agctataact gatgggttaa tagatggtac aacttatatg tctgctgtag
    12601 aagaaggtgg attagtcata gaaaaggcat ctaaattcct taaaggtgaa aaaatagaac
    12661 ctgttacaca aattaaacaa gttgaagtta acaaagataa tatttcagag ttcaagggtg
    12721 aatggtaaat tgtcatagat aataaataag atttttagag ttactttaga aactatataa
    12781 agtaactctt tttttaattt aatttagtga taaattattg aatagtattt ctaaacaaat
    12841 aaaaaattaa tataaacaaa gacttaatta tactaaatta taagtaaaac taatataaac
    12901 ttatggaaaa aaggaaaacg tctaaagtat tggaattact atagtgtagg tgtggtatta
    12961 aaaaaacata aaaaaacata aaaaaattgt tgaaaaaaat atagaaattt tgtataatta
    13021 aagatttgaa aagtgcggta tacaaagcca attcttttgt atataatata tataaattca
    13081 gaaaaaggaa gggatatgta tggccaggcg gttattttta gaaaggattg gacttatatt
    13141 tttaggttca gcaatattag catttggagt ttataatttt tattatttaa acaacataac
    13201 agaaggtgga gtgttaggta ttttactact gttaaaaaat ttattcaata tacaaccagc
    13261 aatagcaaat gttgtaatag atagtttact acttttagta ggatataaat ttttcggtaa
    13321 aaaattcctt atatattcaa tagtagcttc tataactttt tcagtgttat atgacttatt
    13381 tgaagctata ggacctctag taccacaatc acaaaatatg cttttatcaa ctattttggc
    13441 aggtgtaact gttggaactg gtgtaggtat agtagtaaaa gcaggttgtg catcaggagg
    13501 agacgatgct ttagcattag taatttctaa gactacatct ttgaacatag ggcaagttta
    13561 tttagcaaca gatgttattg tattgttgtt atcattattt tatctatcag cgtttgatat
    13621 cttttattct ttgattgctg ttacaattag tggcaaggta attgatttta tttattatca
    13681 tggtaaaagt ttggatatgg atataagtaa tgatatagtt ccagaatgtt aatataaatt
    13741 aaataacttt agattttatt aggggtggca gatttgccac cttttttttg tctaaatctt
    13801 tatagaaagt ataatcccat ctaggttttt gcaaatctca aatatagaat tatgttggaa
    13861 tgactgggaa tacgattgct agatgtgcat tttaaaaacc caattttata tttatgaaat
    13921 actaatcatt aatggatgtt tcttaatcct atctttatac ctaaaatgat aagataaatg
    13981 aagtagcaat acaaagctaa aatttgtaaa agagattaaa ttaaatggag gagctaaaat
    14041 gttggatata gttatgatag cattgttatt cataggattt gtttccatga agttacttgc
    14101 tgattggtgt gggaaacaaa ctgaaaaaaa ataatgtaat gtatggaggg attacctatg
    14161 tggtttctgg cagttgtaat tataatgtta ataatatatt tggtatatgc gcttattaat
    14221 cctgaaaaat tttaaacagt ataaggctaa taagtaagtt gaattggggg gaacaatttt
    14281 tatggtgcag atagtaatag tgttagcgat ttttatgatt ttagtaatac ctatgggcaa
    14341 gtatttatat catatagcta ctaatcaaaa aacatttgga gatagactat ttgataaagt
    14401 ggacaatttt atatataagg tatgtagtat agataagaaa aaagaaatga actggaaaca
    14461 gtatgcatta gctttattat ttacaaatgc agttatggta tttataggat atatcatact
    14521 taggactcaa agtatgcata tttttaatcc aagtggaatt aaaagtatgg aacaaggact
    14581 atcatttaat acaataataa gttttatgac aaatacaaat cttcaacatt attcaggtga
    14641 atcaggactt tcatacttta gtcagatgac agtgataata tatatgatgt ttacatcagc
    14701 tgcaacagga tatgcggctg ctatggcatt tgtaagaggt ctagtcggta aaaagaaaac
    14761 attgggaaat ttttatgtag atttaattag gataacaaca agagtacttt taccaggagc
    14821 tttaattatt gggcttatat tagttacaca aggagtacca caaacttttg ctgggacaga
    14881 aacagtgact actatagaag gaaagcttca agatatagct agaggtcctg tggcagctct
    14941 tgaatcaata aaacatttag gaacaaatgg tggtggattc tttggttcaa attcatcaca
    15001 cccatttgaa aatccaacaa taatatcaaa tatagtagaa atattatcta tgatgatatt
    15061 accaggagct tgtgttgttg cattcggtca catgataaag aataaaaaac aaggttgggt
    15121 tgtgtttggc gcaatgtcaa tcatattttt aataggtcta gtagtgtgct ttaaagcaga
    15181 aagtgcagga aatcctattt tatcacaatt agggcttaat caaagtatgg gaagtatgga
    15241 aggtaaggaa gttagatttg gtatagcaca atcatcatta ttcacaacag tgactacttc
    15301 ctttacgaca ggtacagtta ataatatgca tgatacactg actcctttag gaggattagt
    15361 accactttta aatatgatgc taaatgtagt atttggtggt aaaggcgtag gtctgatgaa
    15421 tatgttgatg tacgctataa tagcagtatt cttatgtgga ttaatggtag gaagaacacc
    15481 agagttttta actaagaaaa tcgaaggaaa agaaatgaag cttattgctt tacttattat
    15541 attacatcca cttttaatac taatgttctc tggtctttca gtagcaatac cagcaggact
    15601 tgaaggaatt tcaaatcctg gattccatgg attatcacag gtattgtatg agtttgcatc
    15661 ttcagcagcc aataatggtt caggatttga aggtctagga gataacacta tgttttggaa
    15721 tataacaaca ggtatagtta tgttctttgg aagatatgtg tctattatag tactactagc
    15781 aatatcaagt ttgttagcat ctaaaaaagc tgtaaatgaa agtataggaa cacttagaac
    15841 ggataatttt acatttacaa tagttttagt acttgttgtt ttaattgttg gtgctttaac
    15901 atttttccca gccttagcgc ttggacctat ttcggagcat ctagtgttgt ggcactaaaa
    15961 aattgggagg gagtaaagtt ttatggaaac taaaaaaaca aagtttatta caaaagatat
    16021 tttcaaaact tccattatag aagcttttaa aaaattaaat cctaaatata tgatgaaaaa
    16081 tccagttatg tttgttgtag aaattggatt tatagtaaca ttgatactta caatagtacc
    16141 tagtttgttt ggtgatgaag gcaataactt aagagcttat aatgggattg taacaataat
    16201 cttatttata acagtccttt ttgcaaattt tgctgaagct gtagctgaag gaagaggtaa
    16261 agctcaagca gatacactta agaaaactag aaaagataca gtagcaaggg ttatagatac
    16321 gttaggaaat gaaaaaatga taagtgcaag tgagctaaaa cttggtgata ttgttttagt
    16381 aaatgctgga gaagttatcc caaatgatgg agaagttata gagggtatag cttctgtgga
    16441 tgagtcagca ataactggag aatctgcacc tgtcatgaga gaatcaggtg gagatttcgc
    16501 atctgttaca ggtggtacta cagttgtaag tgactggtta aagataagaa taacaacaaa
    16561 accaggagaa tcgttcttag ataaaatgat agctcttgta gaaggtgctt caagacaaaa
    16621 aacgccaaat gagattgctt taaatacttt gttagtaggg ttaacactta tatttatggt
    16681 tgttttaata tctctttatc caatggcaaa ctatgcaggt gtaaagattc caatttctac
    16741 tatgatagct cttttggtat gtttaatacc aactactata ggtggattgt tgtcagctat
    16801 tggtattgca ggtatggata gagtaactag atttaatgta attgcaatgt caggaaaagc
    16861 agtagaagct tgtggagacg ttgatactat gattttggat aagacaggta caattactta
    16921 tgggaataga cttgcatcag aatttatagc agttggaaat gcagattcaa aagatttaat
    16981 aaattattct gttatgtgtt cattaaaaga tgatacacca gaaggaaaat ctatagttga
    17041 tttaggtatg aaattaggtt caacaggtaa gacaaaagag gcagaggaag ctgagtttgt
    17101 agaattttct gcacaaacta gaatgagtgg tattaactta gctgatggta gagctataag
    17161 aaaaggtgct tatgatagca taataaaaag agtaaaagaa tctggtggta cagtgccaga
    17221 agatttagaa gaaaatgtaa atcgagtagc taaactagga gggacacctt tagtagtgtg
    17281 tgtagataat gagatatatg gaattatcta cttaaaagat acagtaaaat cagggcttgt
    17341 agaaagattt gctagacttc gagagatagg aataaagaca ataatgtgta caggggataa
    17401 tcccttgaca gcagctacaa ttgcaagaga agctggagta gatggattta ttgcagaatg
    17461 taaaccagaa gataaaatag atgcaataaa aaaagaacaa ttagagggta aaatagttgc
    17521 aatgacaggt gatggaacaa atgatgctcc tgcacttgct caggcagatg taggtcttgc
    17581 aatgaatagt ggtacaacat ctgcaaaaga agcagcaaat atggtagatt tagattcaga
    17641 cccaactaag atattggaag ttgttgaaat aggaaaacag cttttaataa ctcgtggggc
    17701 tttgacaaca tttagtatag caaatgatgt tgcaaaatat tttgcgatta taccagctat
    17761 atttactctt gcaatacctc aaatggagat attaaatgta atgaaacttt ctacatcatt
    17821 tagtgcgata ttatcagctt taatatttaa tgctataatt atcccatgtt taatacctct
    17881 tgcaatgaag ggagtaaaat atagacctat gaaatcacaa gctatgttaa tgagaaatat
    17941 gcttatattt ggtcttggtg gagttatagt accattttta ggaataaaat taattgattt
    18001 attgattaca cctttagtaa tggttcttaa tttaggttaa taaatggagg atataaaaat
    18061 gaaaacatta aagccagcat tacttgtaag tattgtattg ttagttgttt gtggacttgt
    18121 atatccactt gttttgacag gtgtaagcca agttgcattt aaagataaag ccaatggaag
    18181 tatgatagaa gtaaatggtg taaaagtagg ttcagaactt attggtcaaa gctttacaga
    18241 tgtgagattc tttaagggaa gagtatcatc agtaaactat aatacttata caaaagaaga
    18301 tttagtacca gataaagatg gaaacacatc ttatggaggt gtatcatcag ggtcttttaa
    18361 ttatggagct acaaatccag aacttcatga tagagtacaa aaagatatag aacagttttt
    18421 aaaggataat ccaactgtca aaagagagga tataccaaca gatttactta cagcatcagg
    18481 ttcaggactt gaccctaata taagtccagc ctctgcaaaa attcagatac cagctatagc
    18541 aaaagcgtct ggaatatcag aaagtaaatt acaaaaaatt gtagatgata atacttccaa
    18601 aaaacttttt ggagttttag gtgaagatag agttaatgta ttaaaggtta atgtagaggt
    18661 cgcaaaaata ttaggtctta tatagtattt tgtggaattt tagcaacaaa atattacgca
    18721 agatttaatt taaaggttaa attttaaaga gtgaaaatcg ataatgattt tcactttttt
    18781 atttgaatat tactaatatt tggaataaag tgattgtaag tataaaatcc aaaaaaaggt
    18841 tgcattatca aaatgataat actataatat atttatcgtt atcaatttga taatagattt
    18901 ggacatatag gaggaaatag tatgttatat aataacgcat tagagttgat aggaaacaca
    18961 cctgtagtaa ggctgaataa tttaggctgt actaatgggt gttctaacat atatgtaaaa
    19021 ttagaaaaag taaatccagc aggaagtata aaagatagag ctgtttatga aatgttagag
    19081 ggcttagagc aaagaggaga attaaaaaag ggagatgtac tagttgaagc aactagtgga
    19141 aatactggta ttgcattatc tatgatagga aaattaaaag gatatgaagt agtaatcgta
    19201 atgcccgaaa caatgagcgt tgagagaaga gatttaatga aggcttatgg agcaaatttg
    19261 gtgcttacag atggtaaagc tggaatggca ggttccatag aaaaagcaaa tgaactttta
    19321 aaagaaaatc caaactataa aagcctaaaa caatttgaaa ataaagataa tccaaatgca
    19381 cattataaaa caacagcagt tgaaattatg aatgatgtca aagatttaga tatattcgta
    19441 tgtggagttg gaacaggtgg tactttaata ggaactgcaa gatatttaaa agaacaaaat
    19501 ccaaatataa gagtaattgc acttgaacca aaaaaatctc cagctatatc agaaaacaag
    19561 actggacctc ataaaataca aggaataggt gctaactttg ttcctgaaaa ttatgattct
    19621 agtgtagttg atgaagtaat attagtagat gatgaagatg catttgatac agttaaatta
    19681 cttgcagcaa aagagggaat tttagtaggg atatcttctg gagcaaatat atttggtgct
    19741 atagagatgg caaaaaagta tccagataaa aagatagtta cagtagctcc agatggagtt
    19801 gataagtata tgtctatggg aatattttaa taattttatt tgagggagtg aatactttgt
    19861 ttaagaaaat aaataaagat attgaatata taatgaagaa tgatccagca gctaggtcta
    19921 agatagaagt ttttttactt tatccatcag ttcatgcaat gataatgcat aggatggctc
    19981 atgctcttta taagaagaag aagcttttta cagcaagatt aatatcccag atatccagat
    20041 ttatgactgg catagaaata catcctggtg ctaaaatggg ggaaggtata ttgatagacc
    20101 atggaatggg tgttgtaatt ggagagacag cggaagtagg gaatagggtg acaatatatc
    20161 aaggagctac tttaggagct acaggaaaag atactggaaa gagacatcca actgttggtg
    20221 atgatgtatt aattggtgct ggaacaaaaa tactaggacc tttaaatata ggttcaaatt
    20281 caaaaattgg tgcaaattct gttgtcgtta aggatgtacc taatggtgca acagttgttg
    20341 gtataccagc aaaaatagta aaaataagaa acctagaacc tgtaaaaaag aataaaaaag
    20401 aagtgtccta tgagtatgat gaattagata atgtatatta tatataggat ttatataact
    20461 ataaaataaa ctttataaaa attattttaa aaggtatttt tttcaaattt gaggacataa
    20521 taaaaaagaa ctgaatgtta cagttttggt tcatcaatct ttgataaaca agattttaaa
    20581 tttgtagcgt ctaggaggat tttattatga aagcaaatgt taatcaagat acttgtatag
    20641 gttgtggatt atgtccatca atatgtccag aagtatttga tatgaaggat gatgggaaat
    20701 cacatgttat agttgatgaa gtaccaagtg atgctgaaga atcagcagca gaagctagag
    20761 aaagctgtcc agttgatgca atagatgtgc attaataaaa taaatgccta taaaaattga
    20821 attttcaatt tttataggca tttgtctttt attcagttag tatttcaaca cctgtttctg
    20881 taactaaaac catacattcc cattgagcag ataatttacc atcttttgta taaatagtcc
    20941 aattattttc ttcatcaaca aaaatttcgt gagttccttc atttaccata ggttctattg
    21001 tgaatatcat cccaggaacc aatagcatct cagtcccttt tttagataca taacttacaa
    21061 aagggtcttc atgaaagctt aatccaactc catgaccacc tatttctcta acaacagagt
    21121 atccattttt ctttgcataa gagttgatag catcacctat atctcctaaa aatccccatg
    21181 gtttggcaac ttcaagacct ttttggacac attcctttgt aacttgaact aatttttttg
    21241 cattttcatt aacattacct atcataaaca ttcttgaagc atctgaatag tatccattaa
    21301 aaatagtaga aacatctaca tttataatat ctccatcttt taagataatg tccttacttg
    21361 gaataccatg acaaacttca ttatttactg atgtacatac acttttagga aatccattat
    21421 agtctagtgg agcagggata gctccatttt gtagtgtaaa gtcatgaacc aacttattta
    21481 tatcttctgt agacattcca atttcgattt tatctgaaat ataatccaaa atttttgtgt
    21541 ttattacagc actttttttt atttcctcaa tttgactttc attttttata atatcatgag
    21601 ttggtacaat atgacctaac ttagcaaatg aatctatttt attatccata tccatatgac
    21661 attttttata ttttttagta cttccacacc aacagctatc atttctattt aacttcataa
    21721 cttttcatcc ttcttttatt atttatttaa attatgggtt acataaacta ttttaaaaca
    21781 taaccattat aaatattata acataaatac taagaaatcc tttatagata gtgatttatt
    21841 caaagtataa attattatat ggttattgtt gatgaaaaga ggatagttaa atgtattatt
    21901 ggatattagc gcacttaata gtttaattca agtttttcaa gaaaatcata tgttagagga
    21961 caattttcct ttaaagtatt ttttgaatct tcatcaagat aataatgcac aaatgattct
    22021 gcaaaaaatt cttctataaa cttgcttata tatgcatgtg caacttgctt agggtcataa
    22081 agcttatatc tttcctgagc aaatacttcc ataaattctt cagaatttga ttttttattt
    22141 aaaacatttt tatctatagc atgtgcagtt tcatgtaatt ctaaattttt tgatgaatgt
    22201 acatttgcat agctagggtt actataacct attttagcca ctatagaatt atttctatat
    22261 ataccaggta catctttcca agtcttgcct gatcttaccc aacatggagg taattgatat
    22321 tttaaatcac taaattctgg ttcatcagtt agatttgaat ttattaattt tatttttata
    22381 tccttttctt taagtgtctg taaatattta atatttattt ttgcaagtct gtttttcata
    22441 ttttcaactt catctttatc ataattcaag tcatcaggaa gtataagcat ttgttcaatt
    22501 atatctatac tactgtcttt tatattttca gattcttcat taaatttata ttgaagagta
    22561 ttattttcat agatagttat atttgtatta ttttctatag aataagagtg aatatcctct
    22621 aaatctaaaa tattcatttg ttgtaaaata tgttttgttt cgccactcca tactactaaa
    22681 gaaagtgaat taaaattaat tagtggattt tcttttgctt ttatatcatt gcttatatct
    22741 acatcaaata catattcaat tgagtcttct atgttaagac tatctgtatt ctttttaata
    22801 tataaatacg cttgttttaa gtctatataa atctcatcta aagattcgtc ataaaattct
    22861 gataaattat tagtaaagag ttcttcactt aaaagttcat ttttatcagt tatactttca
    22921 attaaaatat taatttcttt tatcatatcg taaatactta tttttctatt tgctttttca
    22981 ctagatacat tgcatggcgc aagagcaatg caataaaaat ctttttcttt tttataaaag
    23041 gctttaagtg attttgataa aagtgtaaag tctacaccta tctgtaagtt gaccttaagt
    23101 gagtcatttt gtaataattc agtattattt cttttagctg tatttaaatt gttttccata
    23161 atcattgccc cctttttatt agggtatcat aattttttta tatgtgtcaa tttgtttttt
    23221 tgaaaattat aatttttttg aaaattttat tttttataat ttgtgaattt tttattatag
    23281 aaataaatat gttattatta tgtcaaaatt tagcattaga ttcatgtaga gtatgtttta
    23341 tacatatatt ataattaatt ctttgaagat tgcatattca aaatacacac catttttttt
    23401 aattctataa tataatattt atatatttaa tgttaggatg tgatttaagg tgtattttac
    23461 atatattata agatgtaaag atgattctct atacactgga tataccagta atatagttag
    23521 aagaatgaat gaacataaat taggaattaa ttcaaaatac acaagagcaa agggatttga
    23581 aaaactagaa gtttattttg tgaccaacac aaaaagcaat gctatgaagt tagaatacta
    23641 tataaaaaaa ttaactagaa ataaaaaatt gtctataata aaaaacccaa gtatacttat
    23701 aaatttaatt gataataaag aagattatat tattggtaag gaaatagaac aattaacata
    23761 aatatttata gtaacattat aatgggtgca tatggtgtaa aaacttgaca ataaatacta
    23821 ttgtaatata atgaagttat aaatatagta aattaaaata gctaggggag ccaagagctg
    23881 agaggtatac cgacccttac acctgatctg gataatacca gcgtaggaaa gcttagtaaa
    23941 aattcaagta tgtattattt gtgatatatg ctatttctaa gctataccta ttgtaggtat
    24001 agctttttta attaagttat ttaaattaaa ctttaggagg caacaatgag taattataaa
    24061 ataccaacac ttacaatagc cggctctgat tcatctggag gagctgggat tcaggctgat
    24121 ttgaaaacgt ttagtgcaat tggtacttat ggtatgagtg taataacagc tataacagca
    24181 cagaatacta aaggagtttt tgctgtagag gatttaaata agaaaataat aaaaaaacaa
    24241 atagaggcag tctttgaaga tatacctcca agagctgtaa aaataggaat ggtttcaagt
    24301 cctgagatta tacttgaaat agttgagaat ctaaagaaat acaatccaaa atatttggtt
    24361 gtggacccag taatgatatc taaaagtgga tattatctat taaagccaga ggcaaaagaa
    24421 aacttaatta agtatttgat accacttgca tacataatta caccaaatat accagaggca
    24481 gaagaaataa caggaataaa aatacataat gtagatgata tgaagagagt aggagaagaa
    24541 atactacaac ttggacctaa atttgtactt atgaaaggag gtcatttaga tggagaagca
    24601 gtggatattt tagttggtaa aaatatattt aaagtttata aaagtgaaag aattgataaa
    24661 aaaaatacac atggaacagg gtgcacatta tcttctgcaa taacatctta tctagcatta
    24721 ggatatgaaa taacagaagc agtaaatcta agtaaaatct atataacaga agcaataaaa
    24781 agaagttttg atataggtca tggagtagga ccagtacatc atttttataa atttgagtaa
    24841 taaaaaaata ttggaggtag aatatgtata atttaattaa agatgtaaaa aaattaaatc
    24901 cattggtaat tcattatact aataatgtaa ctataaatga ttgtgctaat gtaactttag
    24961 ctgtaggagc aagtcctctt atgagttttt catatgagga agtagaagaa atggtaagtg
    25021 tagcaaactc tgtagtaata aatataggaa ctatgaactc aaatatgttg gatttattcc
    25081 tattagctgg aaaagcagca aataagtaca ataaacctgt tgttttggac ccagttggag
    25141 tatttgcaag caaagcgaga gcagaactta caagtagact tttaaatgaa gtcaaattta
    25201 gtgttgttaa agggaatgta tcagaaataa agtttatagg tggctttaat gtaaagggta
    25261 aaggtgtaga ttcatttgat gaagaagaag attctactga gataataaga aagattgctg
    25321 aaaagcttga atgtgtagtt gtagcaactg gaaaaataga tataataact aatggtaaag
    25381 gtacatataa aataaacaat ggaactgata aattaaaagg aattacagga actggatgta
    25441 tgactgctag cttgattgca agttttatgg ctgttactga gaatatatta gaagcagcaa
    25501 caatgggtgt tcttacaatg tctttaagtg gggaattagc taatttaaat aatccaccaa
    25561 taggtacatt taaagaaaat cttatgaatg ctatttatca aatggatata gatactttaa
    25621 gtaaaaattc taatattgaa tttttaaatt aataaagagt aagggagact taaaatgatt
    25681 gataaagaat ctttaaaaaa atgtttaaag ttgtatttag taactgactc tgaaatgctt
    25741 aaaggtagag acttttataa atgtcttgaa gatgcaatat cttctggaat aacaactgta
    25801 caacttagag aaaaaaatgc ttctggaaga gagtttttaa gaaaagctat gaaacttaga
    25861 gaaataacta aaagatatgg tgttaagttt ataataaatg atagagtgga tatagcactt
    25921 atttgtgatg cagatggagt acatgtagga cagagtgaca ttgatgttag agaggttaga
    25981 aagcttatag gaaacaataa aattctagga gtatcagcaa gaactttaga agaagctatt
    26041 tgtgcaaaaa atgatggcgc agactattta ggtgtagggt ctatattcac aacatctact
    26101 aaattagatg caaaatcagt ttcgtttgaa actgttaaag aaattaaaga aaaagtagac
    26161 atgccatttg tactaattgg aggtataaat ttagacaata tagataaact taaatgttta
    26221 gaaagtgacg ggtatgctat aatatcagct atattaaaag ctgaagatat ctctaaagaa
    26281 gtggaaaagt ggacattaaa aatataaaat tggctctggt agtgaaaaaa atggaaaaat
    26341 ttgatataat ctaagagtaa aatttaagca attataaatg ataaactttt ctacatatac
    26401 tttttaatca agatatatgt agatttggtt aaaaaggagc tataaatgat taggatattg
    26461 ttagtgtgtg ttggaggcat gtcttctacc ctattggtaa ataaaatgga gaaggatgca
    26521 aaaaaaagaa atatagactg taaaatatgg gcagttggag aaggagatat aaaatcggag
    26581 cttgataact ttgatatttt acttcttgga ccacaactta gatttatgct tgatgacgta
    26641 aagtctatag ttggagatag agctccagta tctataatag atatggtaaa ctatggcact
    26701 tgtaatggtc atgctgtgtt aaattcggtt ttagaaatat taaaataaat aataaaagtg
    26761 gattctatat aatagagtcc atttttatta tttgttatta ataggcttaa aacttagctt
    26821 accatttctt tttttaatct ccacatagca agtgaaaaac ttgaacagca caatattaaa
    26881 aatagtagga taaaaggcag tatatctaca ataagaatag tctcaccaaa tattaagcta
    26941 cgtaaagcgt taataacatg ggtaaatgga ttgagcatca ctgctacttt taatcctcca
    27001 gataaacttt ctatagggaa aagagctgaa cttaaaaaga aaataggaag tacaattgca
    27061 gtcataattg tttcataaac tatttcattt ggcaataata aacttataga ataagctaga
    27121 cttgataaaa agaaggctgt cataaatatc agtactatca taagtaggat tccaccaatg
    27181 ccagattcta tccttactga gaagaatata cttactatac acaaaatagt tacttctatg
    27241 aaagaaacta agatagcttc taataattga ccaaggacga ttgaatacct gctgataggt
    27301 gcaattaaga ctctatagaa acttccactg ttcttcatta taaagtttat aataccacca
    27361 ctactacaag aagaaaacac aaccagtaca atgatacctg gtagtataaa agcagtataa
    27421 ttattgatat ttatattatt catagtttgg tttgcaatag aactataaag cacaagccaa
    27481 agaaaaggtt gtaatatagt aactacaaat gagattggat ttttaaaacg ccacttcata
    27541 ctacgccata agatatttaa cattcccatt cctcctttaa gcttttttta gaacttgtaa
    27601 gtgctaaaaa tacatcttcg agacttggct ctacaatttc tattgcatca aattctatat
    27661 catgttctaa aagccattta tttattaaag taaaagcagt tctactatcg tttacactta
    27721 taaaaattga attttctcgt acgctcataa actttactaa accagtactt ttaattgaat
    27781 ctttatattt ttttatatct tcagtattat gaaaagttat acgtagtata ttttgtctaa
    27841 tgtaactacg taaacttgat ggtgttcctt gtgctaaatc ctttccattc ttcataatac
    27901 aaatattatc actcaattgt tcagcttctt caagataatg agtagttaga aatatagttg
    27961 tgccatactc atctctaatt tttaataaca tatcccacat agattttctt gaatcgacat
    28021 ccataccaac agttggttca tctaaaaata ggattttagg agatgatacc atattcatag
    28081 ctatgtccaa tctacgtttt acaccccctg aatatgatga agtaggatat tttaagtaac
    28141 tagataagtc aaacttatca attaaagagt ctattctttg ttttgcaatc tgtggttcta
    28201 ctttatacat tttactttga aataccatat tttccataag ggataaatgt tcatcaatag
    28261 agatttgctg tgctacacaa gcaatttgag tacgtatcca agaagaatta tctactaaat
    28321 cttttccaaa catttttaca tttccagatg taggtttgta aaaggtagtc aaaatattta
    28381 taagagaaga tttaccagca ccattaggac caagtaaaga aaaaatttct ccagcattta
    28441 cattaaagct tagaccattt aaagctttca caccattttt atattctttt actaaattgt
    28501 ctatttcaat cgctaacata tattattcct cctctttaag tggaaatatt atctctgtaa
    28561 tatagttatc tgggtttcct tttatcaaca tacctggtcc tttaataaat acttctctcc
    28621 aaggagtttg tatttttata ttattttcat gaatatattt aaatatcatt tcatacgttt
    28681 ttggcaaatt actataagag cctgtgtgag taaaaaataa agcttttgtt ttaggaaatt
    28741 cttttgtagt tatacccata cgatttggag tttcagcagt tggaacacat aattccattt
    28801 ctgcaacacc agttttttgg tcaacatcat agtagcaaaa gaatggtgca ccgttagact
    28861 ttccttttat agctttaaat acatttggaa aatactttgt tgcctcagtc ataggtccat
    28921 tataacgcat ggtagccact gtgactgatt cgatattttt gatttcaatt ttgcaattca
    28981 tttgtttacc tcctaatgtg tgttcatatt cttactagat ttttaagcat taatacatat
    29041 aagattttga ttagttgaaa acatataatc ttatctcgtt tgcttgtttt caagtctaac
    29101 ataacataaa atatatatcc tctcatttga tgctccattt tgtaaagtta ttatttaagc
    29161 tttactttag attttttagc aaattaaaat aaagcaattt ataaaataag tatttacaaa
    29221 ttaagtgtat taatcgtata atacacttaa taggatacta gataaagtct gaaataaagg
    29281 cgtatttttt taaagtaagt gtactatcgt attaatacac tagaatagga ggaatcatga
    29341 attttgaatt agataataca actcccattt atttgcaaat agtaaagtat ataaagagac
    29401 aaatagtaac aggagagttg aaaccaggag agacaatacc atcaagaaga gaaatggcac
    29461 ttaatctaaa agtaaactta aatacagttc aaagagcata taaggagatg ggtgatatga
    29521 atataataaa tacttttaaa aattatcaaa gcagtgtaac agttgatgaa aatatattaa
    29581 aaaatttaaa attagagctt ataaatgaat cactatctgt ttttatagaa gatatgaaag
    29641 caataaatgt tagcaaagaa gaagttttaa aaataataga agataagtat tagtttttta
    29701 acaaatagaa aacttagttt agaaagttta tttataagca tattggatgt ggaggatagt
    29761 aaatatgata gaaataaaga atgtcagcaa aacatataaa aggatgcaag ggcttaaaat
    29821 aagaaagata gaagctttaa aaaatgtaag ttttaatata gaaaagggaa aaataactgc
    29881 attacttggt ataaatggtg ttggtaaatc aaccatgtta aaagcaatag caggacttat
    29941 aaacattgat agtggagaaa ttcgtataga tggagaaaaa ataaatgaaa aagtatacaa
    30001 taaattggct tttgttccag atgtacaatc tcactttagc aatacaacaa taaaagaaac
    30061 atttgaattt atggaaattt tttattcaaa gtggaataaa gaaaaatcaa aagaaatgat
    30121 ggacatattt aaattggatg aagatgaaat aattgataac ttgtcaaaag gaaacattgc
    30181 aagagtaaaa ttaatacttg gattttgcca agacccagag tacatacttc ttgatgaacc
    30241 atttacagga attgatttgt ttaagaggga agagtttata ggagtaatag cgcaatatat
    30301 ggaggaaaat caagccatta tcataactac tcatgagata gtagagatag aaagtttagt
    30361 tgatgaggtt gtaattcttg atgaagggca gattataact tcatttaatg cagaagaatt
    30421 aagagaaaga gaaggtaaat ctatattaga taagatgagg gaggtatata agaatgaata
    30481 aacttctata tctaataaat gtagatttga gaagaagcaa taagttttat atagcttata
    30541 tatcattttt ttcattaatt acacttagtt taaatttatt cgaaattaat aaatttaaaa
    30601 attctaaatt tattatgaac ttagcaatag aagattttgg aggtatattt tatggactag
    30661 ggatacttag taatcttggt tttatccaaa gtatattgtt ctttgggata ttgggacttt
    30721 ttatatacta tatttttatg tggaaaagag agttcttttc aaataatcaa agtatttata
    30781 cacttatgat gttacctcaa aataagttta agatttgtat atctaaatca atagccttga
    30841 taacaatgat atatggtttt ttgataagtc agacagcaac attatttata tgtaaatata
    30901 tatttaattt catttttaga aatatgccta taatcaatat gaactttgcc aaagatttat
    30961 attatttaaa cttgaagagt ataccaa
//
LOCUS       DAGSHT010000026        29264 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGSHT010000026 DAGSHT010000000
VERSION     DAGSHT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.027"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            328..672
                     /locus_tag="MJB72_001108"
     CDS             328..672
                     /locus_tag="MJB72_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBY8262233.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            703..2304
                     /locus_tag="MJB72_001109"
     CDS             703..2304
                     /locus_tag="MJB72_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBY8262234.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSDTECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2323..3423
                     /gene="dprA"
                     /locus_tag="MJB72_001110"
     CDS             2323..3423
                     /gene="dprA"
                     /locus_tag="MJB72_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBY8262235.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3456..5543
                     /gene="topA"
                     /locus_tag="MJB72_001111"
     CDS             3456..5543
                     /gene="topA"
                     /locus_tag="MJB72_001111"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBY8262236.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNQILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5708..6493
                     /gene="codY"
                     /locus_tag="MJB72_001112"
     CDS             5708..6493
                     /gene="codY"
                     /locus_tag="MJB72_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBY8262237.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6600..7865
                     /locus_tag="MJB72_001113"
     CDS             6600..7865
                     /locus_tag="MJB72_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBY8262238.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7896..8666
                     /locus_tag="MJB72_001114"
     CDS             7896..8666
                     /locus_tag="MJB72_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8262239.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8775..9221
                     /locus_tag="MJB72_001115"
     CDS             8775..9221
                     /locus_tag="MJB72_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBY8262240.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9239..10432
                     /gene="nifS"
                     /locus_tag="MJB72_001116"
     CDS             9239..10432
                     /gene="nifS"
                     /locus_tag="MJB72_001116"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBY8262241.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10486..10926
                     /gene="nifU"
                     /locus_tag="MJB72_001117"
     CDS             10486..10926
                     /gene="nifU"
                     /locus_tag="MJB72_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBY8262242.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11215..12294
                     /gene="mnmA"
                     /locus_tag="MJB72_001118"
     CDS             11215..12294
                     /gene="mnmA"
                     /locus_tag="MJB72_001118"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBY8262243.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12437..12675
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12743..15382
                     /gene="alaS"
                     /locus_tag="MJB72_001119"
     CDS             12743..15382
                     /gene="alaS"
                     /locus_tag="MJB72_001119"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBY8262244.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15407..15664
                     /locus_tag="MJB72_001120"
     CDS             15407..15664
                     /locus_tag="MJB72_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBY8262245.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15674..17845
                     /locus_tag="MJB72_001121"
     CDS             15674..17845
                     /locus_tag="MJB72_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262246.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18051..18476
                     /gene="ruvX"
                     /locus_tag="MJB72_001122"
     CDS             18051..18476
                     /gene="ruvX"
                     /locus_tag="MJB72_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBY8262247.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18554..18841
                     /locus_tag="MJB72_001123"
     CDS             18554..18841
                     /locus_tag="MJB72_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBY8262248.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19123..19581
                     /locus_tag="MJB72_001124"
     CDS             19123..19581
                     /locus_tag="MJB72_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY8262249.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19698..20435)
                     /locus_tag="MJB72_001125"
     CDS             complement(19698..20435)
                     /locus_tag="MJB72_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBY8262250.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20652..22328
                     /locus_tag="MJB72_001126"
     CDS             20652..22328
                     /locus_tag="MJB72_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBY8262251.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22534..22734
                     /locus_tag="MJB72_001127"
     CDS             22534..22734
                     /locus_tag="MJB72_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBY8262252.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22788..23951
                     /locus_tag="MJB72_001128"
     CDS             22788..23951
                     /locus_tag="MJB72_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY8262253.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24162..24584)
                     /locus_tag="MJB72_001129"
     CDS             complement(24162..24584)
                     /locus_tag="MJB72_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBY8262254.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24605..25441)
                     /locus_tag="MJB72_001130"
     CDS             complement(24605..25441)
                     /locus_tag="MJB72_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8262255.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25739..26335
                     /locus_tag="MJB72_001131"
     CDS             25739..26335
                     /locus_tag="MJB72_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBY8262256.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILSPIYTVLLKIVYLFV"
     gene            26365..27084
                     /locus_tag="MJB72_001132"
     CDS             26365..27084
                     /locus_tag="MJB72_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBY8262257.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27086..27625
                     /gene="scpB"
                     /locus_tag="MJB72_001133"
     CDS             27086..27625
                     /gene="scpB"
                     /locus_tag="MJB72_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBY8262258.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            27787..28416
                     /locus_tag="MJB72_001134"
     CDS             27787..28416
                     /locus_tag="MJB72_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBY8262259.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            28370..28798
                     /gene="ytfJ"
                     /locus_tag="MJB72_001135"
     CDS             28370..28798
                     /gene="ytfJ"
                     /locus_tag="MJB72_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBY8262260.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            29046..>29264
                     /locus_tag="MJB72_001136"
     CDS             29046..>29264
                     /locus_tag="MJB72_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8262261.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLN"
BASE COUNT        11891 a         3027 c         5238 g         9108 t
ORIGIN      
        1 aacttgatta tttattataa ttagaaataa tattatcata gttatgaccg gatacaatgt
       61 attttcaaca tttttcaaat agcttacaac taatataaat aaagataaat atcgtataaa
      121 agcaactaat ttttctctgt tcattaaagg cactcctcca aaaaaataaa cttttcctat
      181 gaaaactaga ataatatcat aaaagaataa agtcaataga agtatttatt aatattgatg
      241 gaaataaaat caatccaaaa acttgaatag taaaattgta tactgaccat aatttaaaag
      301 tgaaaattta atgtattgga gtgaataatg aataataaag aaaaaggtga ttttggagaa
      361 aaagtagcag ttaattattt gctatcaaaa ggagcaaaga tactagaaaa aaattacaga
      421 ttaaagatag gggaaataga tataatagca aaaatggaag atgaaattat atttgtggag
      481 gtgaaatcaa ggtctaacat aaagtttggc tacccttgtg aatctgtaag cttcaaaaaa
      541 agaaaaaaga taattggagt tgcaagttat tatattataa aaaataactt aaataacact
      601 cccataagat ttgatgttat agaggtatat ttattagaaa aaagaataaa tcacataatg
      661 aatgcttttt aaaaaagata atgtttttgg aaaggattaa atatgttaag tattattaat
      721 tcaagcaact tagtaggaat cgacagcttt ttagttaagg ttgaagtaga tgtaagcaat
      781 ggaataccta gttttaatat tgttggtctt ccaggcaaag aaattaaaga agcaagagaa
      841 agagtaaagt cagcaattct taatagtgga tataaatttc caagcacgag aatagttgta
      901 aatttatcac cagcagatat aaaaaaagaa ggagcttttt tagatttatc tataagtata
      961 ggccttttaa gagagttaat aaaaaaagat gaaaattaca taagagaaag tatgtttata
     1021 ggagaattat cattagatgg gaaaataaga aaagtaagag gtatattgcc cataattatg
     1081 ggtgcaaaaa ctcagaacat aaaacgtatt tttataccaa ttgagaacat aaaagaaagt
     1141 ctatttgtag atgaaattga tataattcct attaaaagtt taaaagaatg tgtcgatttt
     1201 ttaaacgaag agataaaagt cgataaagta aggataatgt cttttttgga tgataagtca
     1261 agaaaagaga acgaagaatt agaaaaagat aatagttata tagattgcaa atatactaag
     1321 attaataatg aagaaagtaa atatgatgaa gactttaaag atgtaaaagg aaattatttt
     1381 gtaaagagga gtgcagaaat tgctgctgct ggaaatcata atatgtttat gattggacct
     1441 ccaggctcag gaaaaactat gattgcaaaa agagtaagga caattttacc agatataagt
     1501 gtagaagaaa tgatagaagt aagtaaggtc tatagtatat tgggtatgat aaatgaaact
     1561 aaaggaataa ttgataaaag accttttaga gcacctcatc atacaactac aaagcaatct
     1621 ttaattggag gaggaatgga tgcaagacct ggagaaattg ctttagcaca tagaggtatc
     1681 ttatttttag atgagatagc cgaatttgat agaaaaatac ttgagacttt aagacaacct
     1741 atagaagatg gatatgtaaa tatatccaga gtaaagtaca gtgcaaaata tccttgtaga
     1801 gtactgttag ttgcagctat gaatccatgt ccatgtggtt attatatgtc agacacagag
     1861 tgtagatgta gaagtaatga aattgataga tacataaata aaatatcagg tccattgtta
     1921 gataggtttg atatttttgt agaagttaat tctataaaat attcagattt taattcttta
     1981 aaacaagaag aaagctctca aaaaataaag agaagagtag aaaatgctag aaaaatacag
     2041 ataaatagat ttaagaagga taatataaaa aataatagtg aaattaaagc ttacaattta
     2101 tttaaatatt gtaaactgga aaaagaagct tcaaaaacgg ctgagatgat atttaataaa
     2161 tacaacctaa gcagtagaag ttatacaaaa cttttaaaaa tggcaagaac aatagcagat
     2221 ttagaagaaa gagacttgat aaattcacag tgtattatag aagctttttc atttagaaag
     2281 gcttattata gttattttaa atagtttttt gaggtggatt ttatggaaaa aagagatact
     2341 tatttatggt taaaatcaat tggtggaata acaacaaaaa caattgaaat aatagaaaat
     2401 gaaatagtaa atattgaaga tatatttgat ttttctgaaa aagaaatata taatttaaaa
     2461 aatataagct tgaatattag aaaaaatata gtaaaatata gaggtcacgc ttatttagaa
     2521 aatataaaag aactacttta caaaaaagcg attaaatata tttgtaaata tgataaagaa
     2581 tatcctgaaa atttaaagaa tatatacaac gctccaaagt tattattcta caaaggtgac
     2641 ataggcttag ttaataataa ttttaatata gctatagtag gttcaagaaa acctacagcc
     2701 tatggaatta attgtgcaaa aacaataagt tgtcaactat cccaatatgg agttaatata
     2761 gtaagtggat tagctatagg aatagatgca tactcgcaca taggttgtat gagtggaaaa
     2821 tctaaaacta tagcagtact tggttcagga gtagacaatc ctcttccaaa acaaaattta
     2881 catttatcaa ataaaatatt agaaaatggg ggattattat tatcagagta taatattaac
     2941 tcgacagtag ccccgtacca tttttctaat agaaatagaa ttatcagtgg tttaagtgat
     3001 ggagtagttg ttgtagaagc agcaataaaa agtggtgcac taatcacagt agattttgct
     3061 cttgaacatg gaaaaaatgt ttttgcaata cctggtaata taaattctca aatgagtaga
     3121 ggttgtcata aaattataaa agaaggtgca aaattaattg aaaatataga tgatatttta
     3181 aatgaatata acatatttaa tattattgat aaaaaaataa atcaaaaata tgataatata
     3241 agtctaaatg ccaagtctaa gcaaataatt gaagctataa aaagagaagg aaacttgcat
     3301 atagatagta tttgtgatta tactggtata gaaataaagt atgtaaattc aataatcaat
     3361 gaattagtac taaatgagtt agtagtagaa atgaataata aaacgtacag tttaaatgta
     3421 taaattaaga ttaggtagtg ggggtgtatg gttttatggc caaaacatta gtcatcgtag
     3481 aatcgcctgc aaaagctaaa actatagaaa aatttttagg gaaaagtcat tacacagtta
     3541 aagcatcagt tggtcatgtc agagatttac ctaaaagtaa attaggtgta gatatagaaa
     3601 ataattttga acctcaatat ataaatataa gaggaaaagg cgatgtaata aaagaattaa
     3661 aaaaagaagc taaaaaatct aaacaagtat atctagcaac tgaccctgat agagaaggtg
     3721 aagctatatc atggcattta gctcatatac taaatttaga tgaatctgag gattgtagaa
     3781 tagagtttaa tgagataact aaggatgcaa ttaaaaaagc aattaaacat cctagaaata
     3841 ttgatataaa tttggttgat gcacaacagg caagaagagt gctagacaga cttttaggtt
     3901 atcaaataag tcctatatta tggcaaaagg ttagaaaagg tcttagtgca ggtagagtgc
     3961 aatctgttac aacaaagtta atttgtgaca gagaaaaaga aataaaggca tttattccaa
     4021 aagaatactg gacaatagat gttgaggcta agaccgtaaa agacaaagat ataactttga
     4081 aattttatgg taaaaatgat gaaaagattg aattagaaaa tgaagaagct gtaaatcaaa
     4141 tactaaaaga aatggaaggt aaagatttaa aggttgagaa gatagaatct aaatcaagac
     4201 gtaaatcagc tccaaaacca tttacaacaa gtatgcttca acaagaagga gcaaataagt
     4261 tattttttac tactaaaaaa acaatgatga tagcacaaga attatatgaa ggtatcgaca
     4321 tagaaggtga aggtactgtt ggtcttatat catatataag aactgactct aaaagaatat
     4381 ctgatgaagc aaaagaaaaa gctaagggct atatcgtaaa caatttagga gaaaactact
     4441 ataaaaacac tggtgataaa aaagaaaaaa aggatactaa aaaggttcaa gatgcccatg
     4501 aggcaataag accaacctca gttgatagaa ctcctgagag tataaaagcc tcattgagca
     4561 aagaccagta caagttatac aatttaattt ggagaagatt tgtagctagt caaatggaag
     4621 attcagtgtt tgatatacta aatgtagaat gtaaaatagg cgatattgta tttaaggcaa
     4681 ctggttcaaa aatgaaattt gatggttata ccaaggtata taatttttca gaaagagaag
     4741 acaaaatttt accaagtata gaaaaaggag atatattaaa tatagaaaat atattgccag
     4801 accaacattt tacacaacca ccagcaagat atacagaagc tagtttagta aaaacactag
     4861 aagaactagg tataggtaga ccaagtacgt atgctccaac aatagctact atactgaata
     4921 gagagtatgt tgaaaagcaa ggaacaagtc tatgtccaac agaacttgga ataatagtta
     4981 ctgatatttt ggaaaataat ttccataaat ttatagatgt agattttact gctgatatgg
     5041 aaagtaaact tgatgttata gaagaaggca atacagactg gaaaaagatt gtatcagaat
     5101 catatgcacc acttaaagag gctattgaag aagctcttga aaatatagaa aaagtaaata
     5161 tggatgaaga aactgatgag atttgcgaga actgtgggtc taatatggtt atcaaatatg
     5221 gtagatttgg aaagtttatg gcttgtaaaa actatcccga ttgtaaaaca actagaccaa
     5281 ttatgaataa aattggtgtt aaatgtccaa aatgtgaaga aggagatatt atacttagaa
     5341 aatctaaaag aggtaaagct ttttatgggt gttctaatta tccaaactgt gactttgtag
     5401 cttggaataa gccaacagga gagttttgtg agaagtgtgg ttcatatatg gttgaaaaag
     5461 tgactaaatc tgaaacaaaa acagtgtgtt ctaataaaga atgtaataaa gagacaaaag
     5521 agcataatga cggaaaagag tgaaaattga aaaaaatcac ataattctct tgaaaaaggt
     5581 tccaatctat gataatcttt caagtgtaaa caactaatag aaaaataaat tttcattaaa
     5641 tgtgaaaata atattagaaa atataaaagg ttattaaaag gcctaaattt catgaggaga
     5701 tgattaaatg gcaagtgaag tgttacaaaa aacaaggaaa ataaataaaa cattacaaac
     5761 aagtggtgga agcagtgtct cttttgattt actggccgga gcattgggcg acgttttaag
     5821 ttctaatgtt tatgtagtaa gtgcaaaagg taaagtacta ggtcttcatt taaatgatgt
     5881 tcaagacagt tcagttatag aagatgagta tactaagcaa aagaaatttt cagatgaata
     5941 tactcaaaat gtgttaaaaa ttgatgaaac attagaaaat ttaaatggtg agaagatatt
     6001 agaaatcttt cctgaagaac atggaagatt acaaaaatat actacagtag ttccaatatt
     6061 aggaagcggt caaagattag gaacattggt actttcaaga tattcaaatt cattcaatga
     6121 tgatgattta gtaatagctg aatacagtgc aactgttgtt ggtcttgaaa tattaagagc
     6181 aataggtgaa gaattagaag aagaaatgag aaagaaagct gtagttcaaa tggcaatagg
     6241 cactctgtcc tactccgagc ttgaagcagt tgaacatatt tttgctgaat tggatggaaa
     6301 agaaggtcta cttgtagcaa gtaagatagc tgatagagtt ggtataacta ggtctgtaat
     6361 agtaaatgca cttagaaaat ttgagagtgc aggtgtgata gaatcaagat cattaggtat
     6421 gaaaggtact catataagaa tacttaatga caaacttaca gatgaattaa aaaaattaaa
     6481 aaacaatcaa taatctatat tttataggtt tagattagaa aaggtatcta tttcaaatag
     6541 ataccttttt tagacgaatt attaaggtaa tgataagtat attaaaaagg agtgagaaaa
     6601 tgcctttagc agataaaata agaccaaaaa caatgaatga tgtaataggt caaagtcata
     6661 taataggtaa tggaaaaata ttgtctaaga tattacaatc taatttttta ccaaatatga
     6721 tattttttgg tcctcctggt gttggtaaaa ctacagtagc ggaaataata gcagaaagaa
     6781 gcaataaaag cttctataaa ataaatgcta ctaattcatc tttagaagat ataaaaaagg
     6841 tgatatcaga gcttggaagt ataaataatg taaatggagt cctattatat atagatgaaa
     6901 tacaaagttt taataaaaaa cagcaacaat caatattaga atttatggaa aatgggagta
     6961 taactttgat tgctagcact acagaaaatc catatcatta tgtatataaa gctcttttaa
     7021 gtaggtctac tgtatttgaa tttaaacctt tagaaaaact tgatatagaa aaaggactga
     7081 aaagagctgt tgaagtatta aatgaagata gctatatgga tattgagtgc aataataatg
     7141 ctattgaaga tatagcaatt ctttctgatg gagatatgag aagagcctta aatattttag
     7201 aagtggtagt ctattcaact aaaccaaata aggataatat aacttatata gattcagatg
     7261 taataagggc atctacattt aataaaatta taaactatga caaaaatggt gatagtcatt
     7321 atgatatttt aagtgcattt caaaaatcta taagaggaag tgacccacag gcatctatac
     7381 actatctagc aagacttata aaaggtgggg atttgataag tatatgtagg agacttttag
     7441 taatagctag tgaagatata ggtttagctt atccaaatgc tattgtaatt gtaaaggcat
     7501 gtgtagactc agctatgcaa cttggatttc cagaagcgaa aataccatta gcagaagcta
     7561 ctattctttt ggctacatct ccaaagtcta attctgcatg tatagcaata atgaaagcta
     7621 tggatgaact ggataaagag tttgttaagg atataccaaa tagtataaag gatgctcact
     7681 attctggttc taaagaaata ggtaggggtt gtggatacaa atatcctcat aattttaaaa
     7741 atcattatgt aaagcaacag tatttaccag atagcataaa agatagtatt tattacatac
     7801 cacaagaaaa taaaactgag aaaaatataa aaaaatattt agaatatcta aatagtgatt
     7861 tttagactga actttatgta tcgggggtag atattatgat tataaaactt aactcaactt
     7921 atcacagtag agttatgaaa tacttaaaaa aagaacctga gtacaatttg tttataatag
     7981 gggatattga aagatatgga tatggaaata attttttaaa tatatgggca gatgtgggtg
     8041 aacatggtga aataaaggca atattactta aatattttga atttatgatg ttttattctg
     8101 atggagagta tgatgttgaa ggtttttata atctacttcg taatactaat tacgaagaaa
     8161 tatctgggaa aatatgtgct gtagatgctt tagcaaagag attaggactt aataatttaa
     8221 aggtagttga tttttgcaaa cttcaaacta aaaagttttt gatagataat aattgtaatg
     8281 caaaggttaa gagaattagg cttggaaatc taaaaaagac agtaaaactt tatgacttaa
     8341 tagatgaatt tcacagtaca actttagaga atttaaaaaa tggactaagg acaggtagag
     8401 gatattgtat agagattaac aaacaggtgg tgtcaatggc aaagagtact tcagaaaata
     8461 ggactcatgc aatgataatt ggagtaggaa cacatcctaa atatagagct aaaggacttg
     8521 ctacaaaatg cctaattaaa ctatgttcag agttactgag agaaaacaaa attccttgtt
     8581 tgttttatga taatgaagag gctggaaaga tatataaaaa actaggattt gaaaacattg
     8641 gaaaatgggg aatttattca aaataaaaaa ttaaaattaa tcttgacaaa attagtaggg
     8701 tataataaaa ttatcttgaa agtaattcct actaaaacag tatgaattag atttctatat
     8761 agaggtgata atatatgaag ttgtctacta aaggtagata tggactaaaa gcaatgtttg
     8821 aattagcatt aaatcaagac aatggaccag tatcattaaa atttattgct aagaagcaaa
     8881 aaatatcaga tcagtactta gaacaaatat tttcatcttt aaaaaaatcg ggtctagtaa
     8941 aaagtgtgag aggtgctcaa ggaggttatt tgctttcaaa gaatgctgaa gacataacag
     9001 taggagatat acttgtagta ttagaaggac cagttgcatt atctgactgt gtactagatg
     9061 aagatgtatg tgaaaactca aacatgtgtg taacaaaaat agtttgggaa aagatgaaaa
     9121 agggtataga agatgttata gattcaatta ctcttaaaga tatgataaat gactataaca
     9181 aaaataaatt agaaaatgat ataacaaata ttaaaaaata gatttggaga tgaatgtaat
     9241 ggaaaaaaga agattgtata tggattattc tgcaacaaca cctattaaaa aagaagtatt
     9301 ggatgcaatg atgccatacc taacagatta ttttggtaat gcatctagtt ttcacacatt
     9361 cggaagagaa gcaaaagatg ctttggataa agcaagagaa caagtagcag cccttataaa
     9421 tgctgaacct agtgaaatat attttacagc aggtggttca gaaagtgata actggacact
     9481 agagggtgta gcttatgcta ataaaaacaa aggtaatcat ataataactt caaaaataga
     9541 acaccatgca atacttcata catgtgaata cttagcaaaa catcatggat ttgaaataac
     9601 ttatttagat gttgatagcg aaggaaaagt agacttaaag caactagaag attcaataaa
     9661 agatactact atacttataa gtataatgtt tgcaaataat gaaataggaa ctatacagcc
     9721 tattaaagaa atatccgaaa tagctaaaaa acataagata ttattccaca ctgatgcagt
     9781 acaagctaca ggtaatattc cagtagatgt taaagaatta ggaatagatt tgatgagtat
     9841 gtcttcacat aaaatatatg gaccaaaagg tgtaggagct ttatacataa gaaaaggtgt
     9901 aagacttcat aactttgtcc atggtggagc acaagaaaaa agcaaaagag ctggtacaga
     9961 aaatatacct gcaatagttg gatatggaaa agcagcagaa ttagctaagg aaaatatgca
    10021 aaatcatgta gaaactttaa ctcgtcttag aaataagtta atagatggtg tgttagaaag
    10081 aataccttac acaagagtaa atggtagctt agaaaataga ttgccaggaa atgcaaactt
    10141 tgcattccaa tttatagaag gtgaaggaat acttttatta ttagatatgt taggaattgc
    10201 tggttcaagt ggttctgcat gtacttctgg ttcattagac ccttcacatg tacttcttgc
    10261 aataggttta ccacatgaaa tagcacatgg ttcattaaga cttacagttg gagattttac
    10321 aacagatgat gatatagatt atatactaga gaacttacca aaagtaatag aaagacttag
    10381 aagcatgtca ccactttatg atgatgcgaa gaaacaaggt ttagtaaaat agacataaag
    10441 ataaagcata aaaaatagac ataaattgag taggaggaaa agataatgca atatagtgat
    10501 aaagttatgg aacattttat gaatccaaga aatatgggag aaattgataa tgcaagtggt
    10561 gtaggtgaag ttggaaaccc tacatgtgga gatataatga agatattttt agacatagat
    10621 ggagatgtaa taaaggatgt taagtttaaa acatttggct gtggttcagc tatagcaagt
    10681 tcttctatgg caactgaaat gataaaagga aaaactataa aagatgcatt agaacttaca
    10741 aataaagctg ttgcagaagc tttggatggt ttaccaccag taaagatgca ttgttcagtt
    10801 ttagctgaac aagctgtaaa agcagctctt atagattatg ctcaaaagaa caatatccat
    10861 ataccagaat tggatggata tgttatagat gatgctcatg accatgatgt tgaagaagaa
    10921 gaataaaaat aagtgattgt gttatgagaa gtttacaatg attaaaaaga agtattaatg
    10981 atataattag gacataaaaa atagctgttt ttaaaatggc tattttttat tgcctaaatt
    11041 aagtatttta acttgattta gaggttaatt agttaacaaa ataatccata agattcaaat
    11101 aataaagatg attatttttt tagaaaatgg aacaagctat attttaaata agtacaatac
    11161 ttgctctata tggtataata ataagtaatg caaaagatga ggtgaaattt gtttatgaat
    11221 aaaaaagtaa tgataggtat gagtggagga gttgatagct ctgtagctgc atatctttta
    11281 aaacaacaag gatatgatgt cattggtgtt actatgaagt tgtggcaaga tgatgatgtt
    11341 gttgaaattg aaggtggttg ttgctcttta tctgctgttg aagatgctag aagggtagca
    11401 aataaaatag gtataccatt ttatgtttta aattttagag aagtgtttaa agaaaaagta
    11461 atagattatt ttatagatga atatttggaa ggaaaaactc ctaatccctg tatagcttgt
    11521 aataaacata taaaatttga tgatttttat aagaaggcta gacaaatagg ttgtgattat
    11581 gtagctacag gtcactatgc aaagatagaa aaggatgaaa gtacaggaag atatttgcta
    11641 aaaaaatctg ttacagataa aaaagaccaa acttatgcac tttataatct tacacaagag
    11701 caactagagc atactttact accaataggt gactatgaga aagatagagt aagagaaatt
    11761 gctaaggaaa tgggtatggc agtacacaat aagcctgata gtcaagaaat ttgttttgtt
    11821 aaagataatg actatgcaaa ttatgtaaaa aaacattcta aaaagcgtat tgaagaaggt
    11881 ttttttgtag atactaaagg caatatactt ggaaaacata agggtatatt atattatact
    11941 ataggccaaa gaaaagggct tggaattact tttggaaaac caatgtttgt aatagacata
    12001 aatccaataa ataataccat agttcttgga gacaatgaag atttatttaa aaaggaactt
    12061 attgctaaag atgttaactt tatatcaata gatacgttag aagaaccctt aagagttcaa
    12121 gccaaaataa gatattcagc aaaaccatca ccagctacta ttcatagagt aggagaagat
    12181 actattaaaa tagtttttga tgaagctcaa agagctatta ctaaaggtca atctgtagta
    12241 atgtatgatg gagatattgt agtaggtgga ggtataatcg agaaaagttt ataaaaacat
    12301 ctaaaaaatt aaaaaaataa gcaataaagt atattaagag taaataaagt ttataaaata
    12361 aatttattgt atatgaaaat atagtattga ttaattttgt aaaatttagt aatataatta
    12421 gtatattaat taaaaaacaa atacagtgat aaggaatagt aaaatatttg atgtcttaca
    12481 gagagaggga aatgctggaa gcccttagaa tatgatattt gaagcagcct tttagtagca
    12541 atcttgaaca atttatattt tataaagaag atttaagtag agattgacgg tagttacgtt
    12601 atagctaagg ttttccgaag atatcatact ttgtatgata attagggtgg taccgcgatt
    12661 gtcctcgccc ctataaattt atttataggg acgaggtttt ttatttaaac gaaaaaataa
    12721 aatttattag gaggaacatg atatggaaaa gatgggatta aatgaaataa gaagtaagtt
    12781 tttagaattt tttgagtcaa aaggtcatta tgtagcaaat agttattctt tagtaccaaa
    12841 caatgataag agtttgttac ttataaactc aggtatggca cctttgaaaa actacttttc
    12901 aggtgtagaa gtacctccaa gtgtgagaat gtgtacatct caaaaatgta taagaacagg
    12961 agatatagaa aatgtaggta taactgcaag acatgctaca ttctttgaaa tgatgggtaa
    13021 cttctcattt ggagattact ttaaaagaga gtcaatcaaa tggggatggg aatttgttac
    13081 agaatggcta aatataccag aagataagat atgggtgact gtatatgaag aagatgatga
    13141 ttcttatgat atatgggcaa aagaaatgaa tttcccagaa gaaagaatgg ttagacttgg
    13201 aaaagatgat aatttctggg aaataggtac aggtccttgt ggtccttgtt cagaaatata
    13261 ttttgataga ggagaagaat atggatgtga caatccagac tgtaaaccag gctgtgattg
    13321 tgataggtat ttagaatttt ggaaccatgt atttactcaa tttgatagag atgaagaagg
    13381 aaattatagt ctattagaaa acaagaatat agatacaggt atgggtcttg aaagaatggg
    13441 atgtattatg caaggcgtag acactatctt tgaagttgat acaattaaat ctatactaga
    13501 agcagttgag aagttaacag gagtaaaata tggagagaat ccaaaaaatg atatatcaat
    13561 aagaataata actgaccaca taagagctgt aacattctta gttagtgatg gagtgcttcc
    13621 atcaaatgaa ggaagaggat atgttcttag aagactcctt agacgtgctg ctcgtcatgg
    13681 taagttatta ggagtaaaag aattattttt acagaaacta atagatgaag ttataaaagt
    13741 aaatgataaa gcatatccag ttttagttga aaaagaaagt tatattaaaa aagtagttgg
    13801 aatagaagaa gaaaaattca atgaaactat agaccaagga acagaaatat tgaactctta
    13861 tatagaagta ttaaaaaatg aaggaaagac tgtcctaagt ggtcaagaag cttttaaact
    13921 atatgatact tatggcttcc ctatagactt aactaaagaa atattagaag aagaacatct
    13981 atctgtagat gaagaagctt ttaatgaaga aatggaaaag caaaaagaaa gagctagaaa
    14041 tgctagagga aatatggatg gagaaagttg gaaggaagac cctctatcaa aattagaatc
    14101 tacagttgac agtacattta atgggtatag tgaaatttat ggtgaaggaa ctattgaagc
    14161 tatagttaaa gatgatgagc tagttcaaag tgcagaagaa ggcgataagg tatctatagt
    14221 actagataac actactttct atcctgaagg tggaggacaa gttggagatt gtggattgat
    14281 aactaatgaa aacttagttt tagaagtatt aaatactaaa aaaggtgcta ataatagtat
    14341 aaaacatata ggtataataa aatcaggaag aataagtaat ggggacaaag taaagacact
    14401 cgtagataga gaaactagaa tgtcagctgc aagaaatcac agtgctacac atttacttca
    14461 taaggcatta agagaagtat taggagaaca tgttaatcaa gctggttctt tagtaactcc
    14521 agaaagactt agatttgata ttactcactt tgaagcgata tcaaatgaag aattaaaagt
    14581 aatagaagaa aaagtaaata atgttatttt atcatcttta gacataaaat gtgatattat
    14641 gaatataaag gaagctaaag aaaaaggagc tacagcacta tttggtgaaa aatatggtga
    14701 tgaggtaaga gtagtttcta tgggagatta ttctacagaa ctttgtggag gaactcactt
    14761 gacaaatact tctcaagtag gaatgttcaa aatattatct gaaggtggag tagcagcagg
    14821 tgtaagaaga atagaagcaa taacaggaaa agctgtttat gaatacctaa aagaaagaga
    14881 tggaattatc tctgaagttt gtgtaaactt aaaatctaaa gaagataatt taattcaaag
    14941 aataagttct ttattagaag aaaataaaaa cttatctaaa gaattgcatg atatgaaagc
    15001 taagatgagt ttacaatcag ttgattctat ttttgattct aaagttgaag taaatggagt
    15061 taatttgata actaataaat ttgaaggtat ggatatggat acactaagag aaacagcaga
    15121 taatctaaga gataagcttg gttctggtgt agtagtactt gcaaatgtag tagatgataa
    15181 agttaacttt gtagttactg ccactaaaga tgtattagat aagggaatac attcaggtaa
    15241 tattgttaga gaagttgcaa aaatagctgg aggaaaaggt ggaggaagac ctaatatggc
    15301 acaagcagga gctagtgatg tatctaaggt tgaccaagct ttaagctatg caagtgaggt
    15361 tattaagaca caagttaaat aacattcact caaggaggtt tttagtttgg aaaaagattt
    15421 agactacact atgaaatttg aaggaatacc agaagatagg atgagtgtag gagatactat
    15481 tgattttgta tataaagcat tagttgaaaa aggatataac cctataaatc aaattatagg
    15541 gtatctcctt tctggtgatt ctagctatat aactagtcat aaaaatgcaa gagctataat
    15601 taaaaaattt gaaagagatg agatacttga agaagtaatc acgcactatc tgaatagaaa
    15661 gtaggtcaaa taaatgagaa aaaagattat gtcgttgttt gcaatagtca ttatgatttt
    15721 tgtatcaact gctagctatg gttttgctga agaaaaaggc aaggtcatat ttattgatat
    15781 gaataggacc aatcttggaa gtatgatgaa tattccaatc ttgaaaaatg agatagaaaa
    15841 gagaggctat gtagctctca tgaatataag aggggatcaa ggtactgatg atagaaggtc
    15901 ttatgcaagt atgggtgcag gaacaagagc tagtgtaact actgaagact atatcaattt
    15961 tcaaaaagta gaccaagata ctgctaaagc ttttgaagtc gcgacaggtc aaaaagctaa
    16021 agaaataaat gatttaggca ttaatagatc tataaatgaa aatcttgagt atggagaata
    16081 tggagcaaat ttagggtcat taggaaaaac tttatcagaa aatggtttaa agacatctgt
    16141 aattggaaat gctgatataa ttgaaaataa tcaattagtt aaaaacagaa acttatgttt
    16201 agtagctatg gaccaatatg gaaggattga ttcaggtaat attgataata ttaataagaa
    16261 agattctaaa atgccatttg gaatatctac agattatgat aaattactag atgaaactaa
    16321 aaaatcctat aaaaatagtg atgctatatt tgtagaactt ggagatacat atagattaga
    16381 tatgtataga ttaaatctaa atgataatac ttatgataca atgaagaaaa atattgtaaa
    16441 aaatataaat aaatatcttg aacaagtatt ttctatggtt ggagaaaatg acactgttta
    16501 tatagcaagt gcatttccaa gtgacctaga ttataagaat aagagaagac tttctccaat
    16561 aataaaattt agtggagaag ggaaaggtct actggaatct gccacaacaa gaaaggctgg
    16621 aattgttgcc aatttagatg ttggagtaga tatactaggt acatttggtc ttgaaaataa
    16681 agagatggta ggtagaagtt atactttagt aaataaagag gacaatatta gctatctaaa
    16741 atcactttat gaaaggatag tgtctatatc ttcaataagg tcaactatta taaatggctt
    16801 tgtaggcgta atatctgctt cttgggtaat tgccatgttt gcgattttct ttagaaatta
    16861 tatgccacat aaagagcgtg tattcaaagt acttaaggag tttataaaat taggcataat
    16921 aatgccacta tcatttatgt tagctccttt gatgaatttt aaaactccaa tttcaatatc
    16981 tttgggaata tttgcaacta cgctaatatt atacttgtta ggtagaaaat tatttaagga
    17041 caatgatata aagcacatgg gattttttgc aacacttact attttagtca tagttataga
    17101 ttctgtattt ggaacatact taatgcaaaa taacataatg agttatgatg ctgtcatagg
    17161 agctaggtac tatggtgtag gaaatgaata tgaaggtgta actatagcaa gtgcagtatt
    17221 tggcttagca gtattattac attataagaa ggtatctaaa ttacttgttg taattttttc
    17281 attaataata cttataacta gtgcatatcc ttcaatgggt gcaaatgttg gtggagctat
    17341 atcagaatgt gtagcatatt tgttgtttat aatgcttata tatgacatta aaatagattt
    17401 taagaaagct gtattattag ctgtttcagc tgtagttgtc gtttttgcat ttgcagcttt
    17461 agatatactt tcaggtagtg aatctcattt aggtatgttt gtacagagaa ttttcctaaa
    17521 tggaccagga gagatattac aaacatttgg aagaaaaatt caaatgaata tgcaactagc
    17581 acagacaagt gtatgggtaa atattttact tgtaggaatt ggagtaattg cagtgcttat
    17641 atttaaacca tctagacact ttagaaagat aatgaataat tttccaatct tgtttaaagg
    17701 gtttattgca tctatggttg gttgtataat tactctttta gttaatgatt ctggaatagt
    17761 tgctgcatct acagcatcta tatatatact tataccacta ataataataa gtataaatat
    17821 gataatattt gaaaaaaata actaaaaaaa tttttactgt tcatatagga tttaaataaa
    17881 aaatgcaata tgattattaa tgtaaactgg atgcttacat aaaaaatcat attgcaattt
    17941 ttatgattga ttatgtaaaa ttctatgaag cagtaagcct gtgaccttag ttatgagagg
    18001 ttcagaatat gtatttaatc tatagaatat aaatagataa aggaggaact atgttagatg
    18061 gaagaataat gggtcttgat gttggtgata aaactattgg agttgctgtg agtgatttga
    18121 tgggattaac tgcacaaggt gtaaaaacaa taaaaagagt tggcaagaaa aaagatattg
    18181 aagagctaaa agcaataata aaagaaaaac aagtaaataa aattgtatca ggattaccaa
    18241 aaaatatgaa tggaacttta ggtccacaag gagaaaaagt aatcaagttt tgtgaattgg
    18301 taaaagcaga aacaggtatt gatgtagagt tttgggatga gaggctttct acagtagcgg
    18361 cagagaggtc tttattagaa gcagatgtta gtagacaaaa aagaaaaaag gttatagata
    18421 tgttggcagc agtaataatt ttgcaaggat atttagattt taaaataaat tcataaaaga
    18481 actataaaat tgaatactta tgatatataa tataaataat aaaaataaaa ataaatttga
    18541 ggtgaatgac attatggaag aaaatataat aaatttaata gacgaaaatg gcgttgaaag
    18601 ccaatttgaa ataatattaa cattagaagc agaaggaaaa gagtatgcaa tattaatgcc
    18661 tttagatgat gaagaagctg aagaagcttt aatctttaga attgatgagg atgaagaagg
    18721 tgaaatctta atacctttgg aaagtgatga agagtatgaa actgttgtag ctgtgtatac
    18781 tgctataatg gaagaagaag gtttaaatta cgacgaagat gaatcaaatg gacttaacta
    18841 gataagtaat taagtttgta tataaaaatc agatatttat tatatttgct ttgattaaac
    18901 cttattaatt gtaaaagcat ttatagtata tatctgtttt tttgtttttt attaaaaata
    18961 ccaaaaaatt tatgaatttt ggtgaataat atatctttta tgggtatata tttatattat
    19021 gagaataaat taaatttatt taaatgtctt gacaaaaaat gaattatcaa ttacaatatt
    19081 atttaataaa gattatcaat tgaaaatagt aagggtgatg ttatggcaaa tacaatggat
    19141 ttattaaaag acaagttaaa agaaactggt tttaagatta cgccacaaag aagagcaata
    19201 gttgaaatat tgcttaaaca tgaccattca catctaagta gtgaggaaat ctatgattta
    19261 gttagggttg actgtcctga aataggctta gctactgtat atagaacaat gcagctattg
    19321 gatgaaatag gattaatatc aaaactaaac ttagacgatg gatgtataag atatgagatt
    19381 agtctacata aagaagactg tcataatcat catcatctta tttgtaaaaa ttgtggaaag
    19441 ataatggaag ctaaagaaga ccttttagac aatatagaaa aagagataca atcattgtac
    19501 aaatttaaaa tacttgatca tgatgttaaa ttttatggac tatgtgacga gtgtaatgga
    19561 gtaagcgatt cagaagaata aacttctatt gcttaatgtc gatggaagaa tagtatagaa
    19621 cttttatatt ctatgctatt cttccatttt ttattataaa ccatctataa aatgcaaata
    19681 tacagttttc aagatgctta tagttttaat attgaaaata taattaatcc tataagtaca
    19741 aaaatgaatg atttttctat gaaattacta aatggtcctc caacagttaa aaaacctaaa
    19801 gaaattttct tttcgcttag cggataaaaa attggtattc cttgctttgt aaataaatct
    19861 cctaaaaaca tatgacttgc ataaccaatc gtaccataaa aagataaatt attaatatta
    19921 gtgattaatt ctatctgttt tagtaaaaaa tatactatta tagttgcaaa tatgctatga
    19981 gaaaaacttc tgtgctttaa ccagggaaaa attgacaaca ttagacaaaa aattgtaaaa
    20041 tatatttcaa cttctatgat gtataagcat atagctaata gtataaatat taatgataga
    20101 aaaacttttc ttagaaatgt gtgggttagt tttgcttcaa taattataat tgcaacaaat
    20161 gtaattattg cgcttaaata aaaattatca tttatattta ttgaaataaa gaaaataata
    20221 atattaacag catatataaa aattctatat atgtatttgg atacattgtt ttttagaatg
    20281 aaatttgaga ccatagaatt agatttatcc aaatcgggta gtacagcaaa agtagctgaa
    20341 actaaaatat caactaaaga tataggaatt ttaaatatta aagatgtttg gatagctgtt
    20401 aagattccta ttgcacaatg agttttacca cgcataaatc ctcctaaatt atccgatatt
    20461 aaatcatata atatttagag gatatcaaaa taaaataggt ttgtcaaaat aatacgttaa
    20521 atgtatttta aatatagctt aaaatatgat acgaattatg gtacaataag aataactggt
    20581 agcgagtgcg tgatttgata ttatttatga ttgtagaacg tgttaaaaaa ttaaatagac
    20641 agaaggagat gatgcaattg ttcaaaaaga acaccaacaa gataaaggta atggccttgg
    20701 gtggacttaa cgaagttgga aaaaatatga ctgttgttga gtataaagat gaaataattg
    20761 ttatagatgc tggacttagt tttccagagg atgaaatgtt aggagtagat atagttatac
    20821 cagatataac ttatttagta aaaaataggg ataaaataaa gggtatattt attacacatg
    20881 gacatgaaga ccatatagga gctcttccat atatattaaa gaaaataaat gtacctgtat
    20941 atggggcaag acttagtata ggtcttatac aagtaaaact taaagaacat aaaatgaata
    21001 atgttaagtt aaatgttata ggtcctagac aagttataaa gcttgataat atggaagtag
    21061 agtttttgaa aaataaccat agtataccag atgcttactc tattgcaata cacacagacc
    21121 aaggtataat ataccacaca ggagatttta agattgactt gactcctatt gatggagatg
    21181 ttatggatat gcatagaatc tgtgagttga gcaaaaaagg agttctttta atgctggcag
    21241 atagtactaa tgcagaaaaa ccaggattta ctatgtctga aaagactgtt ggggtaggtc
    21301 ttgacgaatt atttgcaaaa ggaaatggca gaagaattat agttgctacc tttgcatcca
    21361 acatacacag attacaacaa ataataaata ctgctgaaaa gtttaataga aaagttgcta
    21421 tatcaggtcg ctcaatggtc aatgtagttg gagttgcaaa agaattagga tacctagata
    21481 tttctgatga catgcttata gatttaaatg atatatgtaa gtatgaagat agtgaattag
    21541 tgataataac aactggttca caaggtgaac ctatgtcagc tcttgctaga atggcttttt
    21601 cagagcacaa aaaagtagaa ataaagagtg gcgatttagt tataatatct gctcatccaa
    21661 taccaggaaa tgaaaaatta atatcaagag taatcaattt cttatttgaa aaaggagcag
    21721 aagtagtata cagtgatata gctgatatac acgtttctgg acatgcctgt caagaagaat
    21781 taaaacttat acatgcatta gttagaccaa agttttttat gccagctcat ggtgaatata
    21841 gaatgttaaa aagacatgct gaaatagctg aacagcttgg tatggataaa gaaaatattt
    21901 ttgtcatgca aacaggagat gttttagaat tagataaaaa ttctgctaag gtagcaaacc
    21961 gtatacaaac aggaaacata ttagttgatg gtctaggagt aggcgatgta ggtaatatag
    22021 ttttaaggga tagaaagcat ttatcagaag atggtcttat gatagttgta gttactattt
    22081 ccaaggatga gggtaaggtc ttagcaggtc cagatataat ttccagagga ttcgtatatg
    22141 taagagagtc agaagattta atggatggag caaaggatat aattaagaat gttctaaatg
    22201 aatgtgaaga gaaaaatatt aaagaatggg cttatttaaa gaataatatt aaagaaaacc
    22261 taaaagaata tttatatcaa aaaactaaac gaaatccaat gatacttcct atcataatgg
    22321 aagtataata taaaaaaact ataaatttga aaattagacc tcttgtaatt tttaaagtaa
    22381 tatcaaaaat ccaattgata ttatcttaag aattacaaga ggtcttttta atactcattt
    22441 taaaaaaata tatattacat aaaataccaa ttataacaat cttttattag taaaaactac
    22501 ttatatataa attttactga taggaggatt cttatggatg atgtttcaag acaaaatgct
    22561 ataaaagctt taaaacaaac aaaaatggaa atagcaggag agtatggaat gaattatgaa
    22621 gatgcttttg aaataataga aaatgcgtct aataaaggtg ttttagaagg ttactttaaa
    22681 aagcttgaaa agaaaaaaaa tttaggtcaa ggtatatcta ggcatttaga ataaaaaaag
    22741 ttaaatgtga gaaaataagt ttaaataaac tttaagaggt gacatcgatg aaaaaattag
    22801 ctagtactgc tttggctata ttaattgcgc ttactccttt aagcttttct tttgcaaata
    22861 ataaggaaaa tgctgatgcc aaccaactaa atatatcttc aaaatcagca attttgatgg
    22921 atgttggtag tggacaaatt ttatatgaaa aggatgctca taaaaagctt cctcctgcta
    22981 gtgtaactaa ggtaatgact atgctactta tagtagaagc tttggattca ggcaagatta
    23041 agcttgatga tgaagttcaa gtaagtgaaa ctgcatctag tatgggtggt agtcaaatat
    23101 tcctagagcc aggagaaact caaaaggttg atacattatt aaaaggtata gctgttgctt
    23161 ctgcaaatga tgcttgtgtt gctatggctg aacacttagc tggaagtgtt gaaggatttg
    23221 ttgatagaat gaatgctaaa gcaaaagagt taaatatgaa tgatactcat tttgcaaata
    23281 caaatggact tccagttgca aaccactata catctgctca tgatatagct ttaatgtcta
    23341 gagaactatt aaaacacgaa atgataagta aatacttaac tacatggatg gataaggttg
    23401 tagtagggaa gaaacaagtt acagtaggtc ttgcaaatac taataaactt ataaagcatt
    23461 atcaaggagc aactggtgtt aaaactggtt ttactcaaga agcaaaatac tgtttatcag
    23521 catctgcaaa gagaggtaat actcatctag ttgctgcaac tttaggagca gagacttctc
    23581 cagaaagatt taatgatgcc tctagccttt taaattatgg atttgctaat tatgaaagtg
    23641 ttaagttatg tagtaaaggt gataatatag caacacttac attggataag gctgatgaaa
    23701 acaaagttaa attggttgct aaagaagatt taaatgctct tattaaaaaa ggaagttcta
    23761 aagaatttga gaagaaaatt gaaatagtaa aaaatcctaa gatgccaatt aaaaagggaa
    23821 ctgttttagg aaaaataaag atatgtaaag ataaaaaagt aataggcgaa gtggaattga
    23881 taaatactaa ggatataaat aaggcaagct atctacaaat gcttcaaaga ataattgata
    23941 atatgatata aataattatc agatgaaata taattataat acacaaaaat atgattataa
    24001 tacacataaa aaataccctt atgtaattat ttgccatagg ggtattttta tttaccatat
    24061 agaagtattt tataatctta tgatttttat agaaatatag aaaagtttat gtatcttaaa
    24121 atatcatttg atttaatatc atatataggg ttgtttaaca attatctaag taaaggcata
    24181 ctaccagtaa gtttattcac tttcttttta ttcccataaa ttgctatccc aaaatactgt
    24241 aactcatctt ctggaacact agcaatcttt tgtgtaaatt cttcataaag attgcaactt
    24301 tgtgctacat ctgaaaaatc tacaacagtt aaatcgttaa attccaaagt atataatttt
    24361 ttacgtaaat cttttattat ttctttgttt ccttttaaaa taggtacagg aatgtcaata
    24421 attcctaagt ggctattacc tgttttgtca attacatctg ggccaacagt ttctggggca
    24481 tgttttccta gtgtaattcc cattatagct gctgtattgg atataattcc cattggtaaa
    24541 ttttcgtcta ttaccatgac acattttgaa tttttagttt ccatttttat ttctcctttt
    24601 taccttattt aaagttgttt gaatcatcat ttataaatat attttgatac tgctttggtg
    24661 taagaccaat aaaattctta aaaaagtttg taaagtgact ttggtctaca aatcctgttt
    24721 gtatcgcagc atctataggt tctacgcctt tttctaaaag ctttttagct ttgtttaccc
    24781 gtatattttc taggtatcta tatggtgtaa taccttttaa tttcgtaaaa ttacgaagta
    24841 gagagtactt attcatacct gctatagtgc ttaattcgtc aagtattata tgttcagcat
    24901 aattattttc tagataatca catactgctt gaatttctat atttgtattt tccaagttag
    24961 attgcaaatt tggctctgta tgttcttcaa ttaattgttc aattaggaag aaaaaaagct
    25021 cttccttttt aaaatccaat tcttcttcca tgattatata gtgtaattct tgtagtagag
    25081 gtactagctc actgcgaaat ataacaggtt gattaaattt tggtaaataa tttttacctg
    25141 taatttcaaa gacggttttt ttcattattt ctggcttaat attaatacat ctgtaatcaa
    25201 gtactttatc atctatttgt tcacaagtat gactatcaaa agggttaaaa agtagcaaat
    25261 ctccagtact tgtggtatac tctttatttt tacaagataa atatctttga cctttttcta
    25321 taaaaccaat tacataatgt tcatgaaaat ggtttgggaa tttttgcatt atgcctttaa
    25381 atctatatgc ttcaattttt aattccctat caaagcatac tgtgcgttct tcttgtctca
    25441 cttaggtaac ctcctttcat gttatacaaa gtgtaacaca tattttttta tacttcttgt
    25501 ataatattga ctaagagaca ataatgtcaa ttcatgtcaa tataatatta tacttacagt
    25561 aggtgtaatt attatatttt cacaatgaat atgatataat aataaaagtt ataaaataaa
    25621 aagcaaattt taattttatg cctagaagtt aaaattatta taatgtaaaa taattttgat
    25681 tataagctat ttaattgtta taataatatt aaaaatttaa tttaggagat gtacattaat
    25741 gttaagtatg aatataggag aaatagtagc aagtttagtt ggtatagcta tggcaatatc
    25801 aatacatgag tttggccatg cttattcagc tcacttattg ggagatgata cagctaaagc
    25861 ttatggtaga atgacattaa atcctgtaag acacgttgac ccaataggtc ttttggctat
    25921 gtttatcctt aagatagggt gggcaaagcc agtaccagta aatccaaata actttaaaaa
    25981 ctataagata ggaaacctga tagtttcact tgcaggcgtt ttttgtaatg ttttgactgc
    26041 tatactgtgt gtaattatca ataagtatgt gcatatgtat gctataaata caatagctgg
    26101 atatgtgctt ttatatagta tgggatttgc agcttttaac ttacttccga tacctccact
    26161 tgatggatgg ggagttatat ctacatttgt accatataaa tggaatgaat atttgtataa
    26221 atttgaaagt atgagttata taattttatt aattgcttta tttactggga tgtatagaat
    26281 aatactatca ccaatatata cagtgttgct aaaaatagta tatttatttg tttaatataa
    26341 taaacagatt aaagagggat tgatatgaag tataatatac aattacaagt ctatgaggga
    26401 ccattagatt tactttatga tttaataaca aagcataaaa tagatataaa ggatatatcc
    26461 ataatagata taactaagca gtatctaaat tatttgaaaa tgctagataa aatggattta
    26521 gagataacta gtgaatttat aactatggca tcaaaacttt tagagataaa gtctaaatat
    26581 ctgctttata agcaaaaaga tgaagaagaa gacccaagaa tagaacttat ggaaaagtta
    26641 gaagaatata gaaagtttaa ggtagcatca caagatataa aagaaaatat aacttatgtt
    26701 aatgagagat tttatagaaa taaagaagag ataattattg atgacaatgt ggatttagaa
    26761 gatatatcaa ttgaagcaat aaaaaatata cttccataca tatttaaagt taaaacatca
    26821 cagatagaaa atgctaatga tgagaaacta gataaaatag taaggaagaa aataatatct
    26881 gtagaagaaa aaatattata tataagagat attataaaag ataagattga agttactttt
    26941 acaaatatta taaaatcata tgaaaatgat gaaataatag ctactttttt atctatatta
    27001 gagcttataa aagaaaaaga aatagtcgta gtacaagata tattttttga tgatattcta
    27061 ataagaaaaa gttcggagtg ttaaaatgaa aagagaagat ataaagtata ttatagaatc
    27121 tgttatgttt gcatatgggg aaccaataag tataaaagaa ttaaactata taataaataa
    27181 agaactttca tcaaaagaaa tagagattat gttaaattta cttattgagg agtacagaga
    27241 acaaaataga ggtattcaaa taatcaagtt agagaataag taccaaatgt gtacaaataa
    27301 agattatgca gaatatataa agaagattat tgaaccaaag aaaaagaaaa gtttgagcca
    27361 agctacatta gaaactctta ctataattgc ctacaaacaa ccaataacaa aggtagaaat
    27421 agaggatata agaggagtaa aatgtgataa ggtattacaa actttgtttg aaaatgaact
    27481 tataagagaa gctggtagat tgaataagat agggaaacca ataatttata aaacaacaga
    27541 tgaattttta aagttgttga atatagaaag tcttgaagaa ttgccaccta tagaaaatta
    27601 tcaagaagtg gcaacaaatg aataaaaata taaaatcaca ataatttagt atgtaatttt
    27661 aaattttaaa agatttaaaa tgctctaagt tgttgtgttt ttttgtataa atataaaatt
    27721 ataattctct taaaataaat atggataatt aagtataaat agtaattttt tgatgataat
    27781 ataaacatgg agcttaatga attatttaat tttaagtatt taataatagc tgtaattttt
    27841 attataagtg taactatgtt gattttaata tctcatataa acatcttaat aatagctgat
    27901 ataaaaaaca aagatatttg tttaaagctt aatataaaat acatgtttaa tttaataaat
    27961 ataaatagac aactttatcc agctgaaaat agtaaaaata atgataaaaa agaggggatg
    28021 aagaataata tagattcaag tatattatta gctgatgacc ttttgagcat ttatagatta
    28081 ttaaaaaaga taaaaataca tgaactttat tcaaatatta attttggaac tggaaatatt
    28141 ggcttaacat cttctgtata tgtattgata aatactttgt atggaaattt atttaatatg
    28201 attgatgcag aaaaaatgta tttaaatgta aatccagatt ttacaaaaga ttatgtttta
    28261 ggaaatataa ggatacatat aaggccaaga ataaaagctt tatttaatat tataataatg
    28321 attaataaaa ttatgaataa aaataaagga aataaggaag gtgatagtaa tgagagcaac
    28381 aggttcgata cagagtctta tggaaacaac tcttgaaaca attaaaggtt ctatagatgc
    28441 aaatactatt ataggagacc ctataaaaac agatactaca gtggttgtac ctatatcaaa
    28501 ggttacaatt ggctttggta taggaggagg agaatactca aaaggatatg atgataaaga
    28561 tagggaaatt gaattaaaaa atgaaaaatc agatacgaat tttgctggag gtagcgcagg
    28621 ggctatatct gtacaaccag tagcatttgt agtagttgaa agtggagaga ctagaataat
    28681 gagtttagat agtaatatca atctagtgga taatatatta tctataactc ctagagtatt
    28741 ggagaaaatt caaaatatat cacaaagtaa taagaatcaa gataaaaaca atatgtaaga
    28801 ataaaatatt tttaaatatg tatatagttt taagtaaagt agtggggtta atataaatcc
    28861 cactattttg ttgtgtactt taaacattta tatctaataa gattttagtt taaaacaatt
    28921 taaatagata ataaagttga tatatagtat aattataaaa agaaaagaag tattatatta
    28981 tagatatata aacgcatttg agatataaga gggagttata aatcattagt tttgaggtga
    29041 atgttatgag cgataaagaa ttatctattt tgttgttaga aagtatgaat aattttcata
    29101 atttaataaa gattattaat aacgaaagat ataaaaaaga taaggttctt acagaaagac
    29161 agttctttgc cttagtaaaa ataagaaaac atgataaaat agagcttaaa aatctaagta
    29221 gagatttgca tgtatctact tctagtctgt gtatactact taat
//
LOCUS       DAGSHT010000027        29261 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGSHT010000027 DAGSHT010000000
VERSION     DAGSHT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.028"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            76..789
                     /gene="ispD"
                     /locus_tag="MJB72_001137"
     CDS             76..789
                     /gene="ispD"
                     /locus_tag="MJB72_001137"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBY8262262.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            786..1271
                     /locus_tag="MJB72_001138"
     CDS             786..1271
                     /locus_tag="MJB72_001138"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBY8262263.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHSI
                     MDSILGALALGDIGKYFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1366..1574
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1638..3353
                     /locus_tag="MJB72_001139"
     CDS             1638..3353
                     /locus_tag="MJB72_001139"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY8262264.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLVSQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3475..4920
                     /locus_tag="MJB72_001140"
     CDS             3475..4920
                     /locus_tag="MJB72_001140"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY8262265.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5360..6841
                     /locus_tag="MJB72_001141"
     CDS             5360..6841
                     /locus_tag="MJB72_001141"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBY8262266.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7062..7217
                     /locus_tag="MJB72_001142"
     CDS             7062..7217
                     /locus_tag="MJB72_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262267.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            7362..8759
                     /locus_tag="MJB72_001143"
     CDS             7362..8759
                     /locus_tag="MJB72_001143"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBY8262268.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8744..9157
                     /locus_tag="MJB72_001144"
     CDS             8744..9157
                     /locus_tag="MJB72_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBY8262269.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9263..10021
                     /locus_tag="MJB72_001145"
     CDS             9263..10021
                     /locus_tag="MJB72_001145"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBY8262270.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            10034..10771
                     /gene="rlmB"
                     /locus_tag="MJB72_001146"
     CDS             10034..10771
                     /gene="rlmB"
                     /locus_tag="MJB72_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBY8262271.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            10774..11301
                     /locus_tag="MJB72_001147"
     CDS             10774..11301
                     /locus_tag="MJB72_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBY8262272.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            11366..12013
                     /gene="sigH"
                     /locus_tag="MJB72_001148"
     CDS             11366..12013
                     /gene="sigH"
                     /locus_tag="MJB72_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBY8262273.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            12087..13280
                     /gene="tuf"
                     /locus_tag="MJB72_001149"
     CDS             12087..13280
                     /gene="tuf"
                     /locus_tag="MJB72_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBY8262274.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVV
                     ATIIE"
     gene            13530..13679
                     /gene="rpmG"
                     /locus_tag="MJB72_001150"
     CDS             13530..13679
                     /gene="rpmG"
                     /locus_tag="MJB72_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBY8262275.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            13705..13926
                     /gene="secE"
                     /locus_tag="MJB72_001151"
     CDS             13705..13926
                     /gene="secE"
                     /locus_tag="MJB72_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBY8262276.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            13946..14488
                     /gene="nusG"
                     /locus_tag="MJB72_001152"
     CDS             13946..14488
                     /gene="nusG"
                     /locus_tag="MJB72_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBY8262277.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            14527..14952
                     /gene="rplK"
                     /locus_tag="MJB72_001153"
     CDS             14527..14952
                     /gene="rplK"
                     /locus_tag="MJB72_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBY8262278.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            15023..15721
                     /gene="rplA"
                     /locus_tag="MJB72_001154"
     CDS             15023..15721
                     /gene="rplA"
                     /locus_tag="MJB72_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBY8262279.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    15773..15901
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            15944..16450
                     /locus_tag="MJB72_001155"
     CDS             15944..16450
                     /locus_tag="MJB72_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBY8262280.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            16508..16873
                     /gene="rplL"
                     /locus_tag="MJB72_001156"
     CDS             16508..16873
                     /gene="rplL"
                     /locus_tag="MJB72_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBY8262281.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            17265..18053
                     /locus_tag="MJB72_001157"
     CDS             17265..18053
                     /locus_tag="MJB72_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY8262282.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLSRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            18524..22240
                     /gene="rpoB"
                     /locus_tag="MJB72_001158"
     CDS             18524..22240
                     /gene="rpoB"
                     /locus_tag="MJB72_001158"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBY8262283.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            22282..25767
                     /gene="rpoC"
                     /locus_tag="MJB72_001159"
     CDS             22282..25767
                     /gene="rpoC"
                     /locus_tag="MJB72_001159"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBY8262284.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            26056..26478
                     /gene="rpsL"
                     /locus_tag="MJB72_001160"
     CDS             26056..26478
                     /gene="rpsL"
                     /locus_tag="MJB72_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBY8262285.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            26602..27072
                     /gene="rpsG"
                     /locus_tag="MJB72_001161"
     CDS             26602..27072
                     /gene="rpsG"
                     /locus_tag="MJB72_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBY8262286.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            27121..29187
                     /gene="fusA"
                     /locus_tag="MJB72_001162"
     CDS             27121..29187
                     /gene="fusA"
                     /locus_tag="MJB72_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBY8262287.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT        11771 a         3373 c         5904 g         8213 t
ORIGIN      
        1 ttaatcagat ggttaaaata aaaatattaa aagttttaat aaatgatggt taaaaataaa
       61 atacgaggtg ataccatgta tagtgttata attgttgcag cgggaagcgg aaggagaatg
      121 aacttagata taaacaaaca gttcataaaa ttaagagaaa aagaaattat tgctcataca
      181 attcaagttt tttatgaaaa tataaatata gatgaaatag tggtatgtat aaaaaaagaa
      241 gaagaggatt tttttaaaga aaatataata aataagtata actttaaaaa tataaaaata
      301 gcatatggtg gaaaagaaag acaggactca atatataatg gtttaaaaaa attagatgaa
      361 aattgtgata tagtacttat ccatgatgga gcaagaccat ttgtcgacca tagaataatc
      421 aatgaatcta taaaggtagc taaagaaaaa aaggctgtgg ttgtaggtgt tccagtaagt
      481 gatactataa aaatagtgtc agatggaaca gtacaagaaa ctccagaaag aaatttgctg
      541 tgggcagcac aaacccctca aacctttgaa tataatctca taatagatgc ttatgaacaa
      601 gcatataaga acaattatta tggtactgat gattcaatgt tggtagaaaa tataggtcaa
      661 agtgtcacta tggtaatggg ttcttatgaa aacataaaaa taactagtcc agaagattta
      721 aatatagcag aacaaatatt aaatatggaa aaaagagatg aagttaacag tagaaggaga
      781 attatatgag aataggatta ggatatgatg tacacaaatt aacagaggat agaaaactta
      841 taatcggtgg tgtagagata ccacacgata aaggtttgtt gggtcattct gatgctgatg
      901 tattgataca ttcaataatg gattctattt taggtgctct agcattagga gatataggga
      961 agtatttccc agatacagat gaagaatata agggagcaga tagtatgaaa ttattagaac
     1021 atgtgtataa tctaataaca agtaaaggat ataaaattgg taatatagac agtactataa
     1081 ttgctcaaag ccctaagatg gctccatata ttgagagtat gagaagtaat atatcaaaag
     1141 ttttaaacac agacattgat aatataaaca taaaggcaac aacagaagaa ggtcttggtt
     1201 ttacaggagc aaaacaggga atagcttcac aaagtatttg tttattattg ttgacaagtc
     1261 agaataatta atatattatt agtaataagt ttaatggata tcaagtaata ggcctatata
     1321 gaatatctat aatgtatgaa tttaagtgaa attaatataa atataaaatt agctatgata
     1381 aagaaatagt aaaatgatga aatcttcaca gagagaaaac ggtggtgaga gttttcaaga
     1441 ggaagtcttt gaacctgtct tttagcttaa aggtataaaa atctttacgg taggaaccgt
     1501 tatattccca aaagagagct attcatatag ctaactagag tggtaccgcg gaaattaaac
     1561 ctttcgtctc tatacttgta tagagatgag aggtttttta tattttcagg aagttaatat
     1621 tcaggaggaa tctattaatg aaaatgtcaa aaatgtttat gccgacatta aaagaaatcc
     1681 cagcagatgc tgagattaca agccatcaat taatggttag gtcaggcatg ataaaaaaaa
     1741 tgacttcagg tgtttataac caattaccaa tggggttaag agtgttcaaa aaaattgaac
     1801 aaataattag agaagaatta aataaaaaag attgtcaaga aatactttgt gccgccttac
     1861 ttccttctga attatggaaa gagtctggaa ggtggactgc tatgggtgaa gaaatgttta
     1921 gactaaaaga cagaacagaa agagagtact gtcttgggcc aacacatgaa gaagcattca
     1981 ctgatataat aagacaagaa attacttcat ataaacaatt gccactgaac ttataccaaa
     2041 tacaagtaaa atatagagac gagagaagac caagatttgg cgttatgaga actaagactt
     2101 ttacaatgaa ggatgcctat agttttgatg tagatgataa aggtttagat aagtcatatc
     2161 aagatatgtt tgatgcatat gtaagtatat ttgatagatg tggattggag aatagccctg
     2221 tacaggcaga ttcaggagct ataggaggtt ctacatcagc cgaatttatg gttaagtcag
     2281 aagttggtga agatgaagtt gtattttgta gtggatgtga ttatgcagcc aatgtagaaa
     2341 gagcagaatc ttgtaatcta gtatcacaaa aagaagaaat gaaagaatta gaagaggttc
     2401 atacaccagg agcagctaca ataaaagagt tagaagaatt tttaaaaact tctccagata
     2461 agtttgctaa aactttagta tatgaggcag atggaaaaac agttgtagtc gttgtaagag
     2521 gagatagaga agttaatgaa atcaaagtat ctaatgctat aggttctgtt atagagtttg
     2581 ctcttgctac agatgatgta gtgagaaaag taactaatgc agaagtagga tttgcaggtc
     2641 caataggtat aaatgctgac tatgtattta tagataaaga aattgttgaa caaagaaaca
     2701 tagttgttgg ggctaataaa actgagtacc atataaaaaa tgctaactat ggaagagatt
     2761 ttgaaggaat agttggagac tttagaaatg ttcaagaagg cgacaagtgt atagtatgtg
     2821 gtaagccact tgaaatagct agaggtgttg aagtaggaca tatatttaaa ctaggaacta
     2881 agtactcaga atctatgaat gcaaacttca tagacaaaga tggaaaatca aagcctatag
     2941 taatgggatg ttatggaata ggtgttgaaa gaacagcagc agctataatt gaacaacata
     3001 atgatgagaa aggtataata tggcctttgt cagtagctcc ataccatgtt gtgatagttc
     3061 cagcaaatat gaaaaatgaa gaacaaatat caatagctga aaatatatat aatgatttgc
     3121 aagctatggg tgtagaagtt ttattagatg acagagatga aagaataggt gtcaaattca
     3181 atgattcaga attaatagga ataccgatga ggattacagt aggtaaaaat attaatgaag
     3241 gtaaagtaga atttaaactt agacataaag aagataaaga gataattgac atagaagaaa
     3301 taaatgaaaa agtaaaggca gagtttatca ggaataatgt taggttagga taataactta
     3361 aataagatat tgtatagata atcaaagttg tactttatag acaccatttt atctatatat
     3421 aaggatataa aatggtgttt ttttataaaa caaataaatg aggaggtaat tgaaatggca
     3481 aaaaatgaaa aacaatttgt tgaagaaata acaaagatgg aagatgattt tccacaatgg
     3541 tacacagatg taataactaa gactgactta gttgattatg caccagtaaa aggttttatg
     3601 gttgtaaagc catatggata tgcattatgg gaaaagatgc aagaatttat ggataaaaaa
     3661 tttaaagaaa ctggacataa aaactgttat tttccattat taataccaga gagtttatta
     3721 aacaaagaag cagaacacgt tgaaggtttt gctccagaag ttgcatgggt aacacatgga
     3781 ggaaataaaa aattagaaga aagattatgt gtaagaccta cttcagagac tataatatgt
     3841 acaatgtatg cgaaatggtt aaaatcatat agagaattac catatcttta taatcaatgg
     3901 tgctcagttg ttagatggga aaaatcaact agacctttct taagaacatc tgagttttta
     3961 tggcaagaag gtcatacgtt acatgaaaca gcagaagaag cacaagaaga aacaatacaa
     4021 caattagaag tatataaagc gctatgtgaa gaattacttg caatgccagt tgtagctggt
     4081 caaaaaagtg aaagtgagaa atttgcaggt ggtgaaagaa catatactat agaagctatg
     4141 atgcatgatg gtaaagcatt acaatcaggt acaagtcact tcctaggtca acactttact
     4201 aaggcttttg atattacttt tgcagatagg gaaggaaatt tagctaatcc ataccataca
     4261 tcttggggag catctacaag acttataggt ggacttataa tgactcatag tgataacaga
     4321 ggtttagtat taccaccaag agtagctcca attcaagttg ttatagttcc tatagctgct
     4381 aaaaaaggaa atgtaatgga gactgttgat aaaatatatg ctgatttaaa agctaaggga
     4441 gtagctgtag aagttgatga tagagataac tatacaactg gatggaaatt taatgagtgg
     4501 gaaatgaaag gtgttccagt aagagttgaa attggaccaa aagatataga aaataatcaa
     4561 gctatggtat ttagaagaga tactttagaa aaggattcta tgcctttaga aggattagct
     4621 gatgctatat gtgatttatt tgatgttata cacaatgata tgtttgaaaa agcaagaaaa
     4681 catagagaag acaatacatc tatagttgaa aatatggatg agtttagaaa agctttagaa
     4741 gaaaagccag gattcataaa gactatgtgg tgtggagatg cagaatgtga agctaagatt
     4801 aaggaagaaa caggagcaac tataagatgc ttaccttttg aacaagaaaa cttaggtcac
     4861 aagtgtgtgt actgtggaaa agaagcagat agtatggtag taatggcaaa agcttactaa
     4921 aatacattta aataaaaact ggggtagaaa gtatttaata ctttatccca gtttttttgt
     4981 tttttatgag taataaaatg tatttgtaac tatgtcataa gataagtcat agatacttat
     5041 attaaaaaga atataataga gaaccatttt taataattac atattaatga aagcaaaata
     5101 tttttgttat atagattttc aataaagcaa acaataaata taaatgaata ttagtaagga
     5161 gtgcttttaa tttttagaaa ataaatgact taaaagaaag atttatgaaa ttaaaactag
     5221 gattttctga tataataaac atgtgtattt ttaaataaaa aggatattat tattagattt
     5281 gattaaaaat aaacttgcaa aacaatgtac tagtttaata ttatagttat attgaataaa
     5341 atatcaggag gaagtaaaaa tgaatgtaag agtaaggttt gcaccaagtc caacaggatt
     5401 tgtacatata ggtagtttga gaactgcttt atataactat ttatttgcaa aaaaaatggg
     5461 cggagaatat atattaagag tggaagatac agaccaaagt agattagtag aaggtgctat
     5521 agagaatatg cttaatgcta tgaaatgggc tggtgtcaac catagtgaag gtgttatatt
     5581 agatgatagt ggaaaagtag tacaaaaagg agaatatggt ccatatattc aatctcaaag
     5641 attagatata taccaagaat atataaagca acttttagat agtggaaaag cttattactg
     5701 tttctgtaca aaagaaagac tggatgaagt aagagatgct caaagagcag caggagaaac
     5761 agctaaatat gatggtcatt gtaagaatct ttctaaagaa gaagtagaag cgaatataaa
     5821 agcaggaata ccatatgtta taagattaag attgccagag aatcatacaa ttaagtttac
     5881 agacttagtt agaggagata tggaatttaa tactaatgac ttagatgacc aagtattaat
     5941 gaaaacagat ggatttccaa cttatcattt tgcagtagtt gttgatgact atttgatgaa
     6001 aattacacat gttataagag gagaagaatg ggtatcatct actccaaaac atgtttattt
     6061 atatgaagct tttggatggg aagcacctgt atttgtgcat ttaccaaata tacttaataa
     6121 agaaaagaaa aaattaagta aaagacaagg tgatgtagct gttgaagact ttaagaaaaa
     6181 aggatattta ccagagggat tagtaaacta tgtagcatta gttggttggt ctcctgaaga
     6241 taatcaagaa ttgtttacaa tggaggaatt agaaaaagcc ttctctgtag aaagagtatc
     6301 taaaagtggt ggagtatttg atactgaaaa attaaattgg gttaatcagc actatataaa
     6361 agatggtgat gatgcttatc taacagactt agcaatacca tttttaatag aagatggatt
     6421 tataacagaa gaagaagcta ctaataaata tgatttctta aagagtatga tatcagtact
     6481 taaagagaaa ttacaatatg taaaagaagt tactgaacat gcaagtatat tttttggaga
     6541 taaaatagag gtagaaactg aagagggaag agagttctta agattagaac atatacctac
     6601 tttaatagat gctttgagag aaaaaataga aaaaacagag gtattaaatg ctgagtttgt
     6661 tcaagctatg cttaaagaaa tacaaaaaga gtacaaaata aaagggaaaa atttatttat
     6721 gggctcaaga ataattttga cagggcaaat gcatggtcca gatttaccaa aagtaatgga
     6781 agttttagga aaagaaactt gcttaaatag aatagcttat gtaaaaaata atatattata
     6841 gtcataaaag tccgctaatt tagaagcgga ctttttttgt gttagtattt ggtgaaaata
     6901 tctgcttaag aaaatgaagg tatttaaggt aataaattta atatataaaa agcaaagcaa
     6961 aaatataaat aaaatatgaa taataaacta ataaaatatt aagcatagaa gaagggaatc
     7021 tattatttta gtttgattta attattaagg gggtaattac tatgtatagt aatattgatg
     7081 atgttaaaaa ggaattaaag gagctatgct tagaatatgt tacaatttta gaaaaattaa
     7141 aagatgaaaa aatgattaca gaagaaactt ttgaaaagtg ctcttctcaa aaaaaaatat
     7201 ttttagaaga acaatagagg atggtaatag tattattatt atgtagtttg aaagatagta
     7261 cgaaaaatga tagtaatact attatttatt gcaattttca actataatat aatatataat
     7321 gttaagaaga taatttataa aaatacagtg aggtgatgag tgtgaaagtt tataatacat
     7381 tgactagaac aaaagaagaa tttgttccat tagaagaagg aaaggtaaaa atgtatgtgt
     7441 gtggtccaac agtttataac tatatacaca taggtaatgc aagaccattt ataatatttg
     7501 atactctaag aaggtattta gagtatagag gatatgatgt aacttatgta caaaacttta
     7561 cagatgttga tgataagatt ataaatagaa gtcatgagga ggggatatct cctgaagaag
     7621 ttgcagctaa gtatataaaa gaatattttg tagactgtga tgggctagga ataaaaagag
     7681 ctactgttca ccctcaggtt acagataata tacaacagat tatagagttt ataaaagagt
     7741 tggaagataa gggatatgca tatgcagtaa atggtgatgt atattttgat acaaataagt
     7801 ttgaaggtta tggaaaactt tcaggtcaaa aacaagaaga tttagaggca ggggctagaa
     7861 ttgaggttaa tgaccaaaaa agacatccta tggactttgt tctttggaag gcaaaaaaag
     7921 aaggtgagcc aggatgggat agcccttggg gagaaggtag accaggatgg catatagaat
     7981 gttctgttat gtctaagaga tatttaggtg aaacaattga tatacatgca ggtgggcaag
     8041 atttaacatt cccacatcat gaaaatgaaa tagctcaaag tgaagctaga agtggaaaaa
     8101 cattctcaaa atactggatg cataatggat atataaatat aaatgatgaa aagatgagta
     8161 aatcaaaagg taatttcttt acagtaagag atatatcaaa attatatgat ttagagatag
     8221 taagattttt catgttatca gcacattata gaaatccagt aaactttagt gatgagatgt
     8281 taaatcaagc taaagcaggt cttgaaagac tatataatac taaagaaaaa ttagaattta
     8341 cgctaagtaa tttagtagaa tctcctttaa cagaaaaaga agtagagctt gttaaagaat
     8401 tagatgactt tagacaaaaa ttcatagatg ctatggatga tgatgttaat acagcagacg
     8461 cagtaagtgt aatatttgaa ttagctaagt taataaattc taatgtagat gaaaattctt
     8521 ctttggagtt tgctaaaaaa tgcttagatg aatttaatga acttacagga gtattaaata
     8581 ttgtaaataa gaaaaaggat actgtattag ataaagacat tgaagaattg atacaaaaaa
     8641 gaactgatgc aaagaaaaat aaagagtttc aattagctga tgatattaga caacaattac
     8701 tagatatggg aattgtatta gaagacacaa gacaaggtgt taagtggaaa aggatataaa
     8761 aatggaaaaa actgaattag ttactatgtc accattagtt ttagcatact taggtgatac
     8821 agtgtatgag acatatataa gagaatattt aattagacag aatacacaaa gaaaagttaa
     8881 tgatttgcat aaactagcta taaagtatgt aaaagctaaa gctcaggcaa ctataattca
     8941 tgaaattgaa attgagttaa ctgaagaaga gagtaaaatt tataaaaggg gaagaaatca
     9001 aaagtcaaat acttctccta agaatgcaga tattatagac tacaaacatg caactgggtt
     9061 tgaggcatta gttggatatt tgtatttaaa taatgaaata gagagacttc aatatattat
     9121 caataaagga atcaaaatta ttgaaagaga tatgtaaata ttaaagagtg ttgtcttaat
     9181 aataagtaat aaggcaacac tttttttata actaatgtat aataaatata tagaaattag
     9241 taatgagaaa ggataaaaag ttatgaaagt aaaattaata tcacatacac ctgaaccaga
     9301 aaaagtaata gctatggcag ctaagctatg ttattcacca gttgggacag acgaaataga
     9361 aaaagactta acagatgaaa gtatagagaa atttttaaat atgttattaa gtataggtca
     9421 tgggtcaata ttagaacatg cttcatttac attttcaata gaaggtattt caagggcatg
     9481 ttcacatcaa attgttagac atcgtatagc tagtttttca caacaaagtc aaagatatgt
     9541 taaattagaa caatttgagt acataattcc tccagaaata gaaaaaatag aaaaggcaaa
     9601 agagttattt atagactcca tgaaaaaaga ccaagagaat tatgataagt tagttgaaat
     9661 attgtttgaa aatcattata atgacctaat aaaaaacggt aaaaatgaga aaacagctaa
     9721 aagacaagct gagaagaaag caatagaaga tgctagatac gtatttccaa atgcatgtga
     9781 aacaaaaatg gtatttacta taaatgctag aagtttgttt aatttctttg aacatagatg
     9841 ctgtgaaaga gcacagtggg aaataagaaa tttagctgtg gagatgttaa gagaggttaa
     9901 aaaggttgct cctatattat ttaaaaagac tggtccaagt tgtgtaaatg gaagttgtcc
     9961 agaaggaact atgacttgtg gagatatagt tcaagttaga gaaaaattta aggccttgta
    10021 ggaggtgaat aatttggcga gtatagaagg aagaaatccg gtaatagaag caataaaaag
    10081 cgacagagaa atagataaaa tattaattgc aaattcagct aaagaaggtt caattaagaa
    10141 aataatagga atggctaaag ataagaatat aattattcag tatgttgata aacataagtt
    10201 agatgaagta agtacaagtc attcacatca aggtgtaata gcttatgcaa gtgagtataa
    10261 gtattatgaa ttagatgagc taatagattt agcaaagaat aaagatgaag acccattttt
    10321 cattatatta gatgagataa cagacccaca taatttgggt tcaataataa ggacagcaga
    10381 tgcagtagga gcacatggag ttatcattcc aaaaagaagg tctgtacaca taactcctgt
    10441 agttgccaaa gcatctgctg gagcagtaga atatatgcca gtttgcaagg taaccaatat
    10501 agttaataca ataaaaagac tcaaagaaga agggctgtgg attgctgcag ctgatatgga
    10561 tggagaaact ttttataaac aaaatcttac tggtcctctt ggtgtagtaa ttggaagtga
    10621 aggttttgga atatctagat tggttaaaca aaactgtgac tttatagtaa aaatgccaat
    10681 gataggtaat gttacatctt taaatgcatc tgtagcaggt ggaatacttt tatatgagat
    10741 attcagacaa agactggata aaagtaaata ggcatgaaaa aaaatataaa tcattactta
    10801 ataatagatg gctacaatat tataaatgcg tgggacaatt tgaaagaact ggctaaagag
    10861 gacttagaag attctcgaga aaagcttatt gatgacatta tagaattttc tgagtttatg
    10921 ggttataaga ctataatagt ttttgatgct tataatgtta aaaattctcg agaaaaagtt
    10981 gaaaaaagaa aacacataac aatagtttat acaagagaac accaaacagc agatagttat
    11041 atagagaaat ttataacttc tttatcaaag tatgatgatg taaaagtagc cactaatgac
    11101 tatgcagaac agcaaatgat tttaggaaaa ggtgctacta gaatgtctgc aagagaatta
    11161 aagttagagt tagatcgttc caagaataaa atgaaggaaa aaaatatcgg tttgaggaaa
    11221 aaaattcagc gaaattggtt ggaggaaagg ttagataaag aaacattgtc gaaacttgag
    11281 aacattcgta gaaagcgttg aaattactag gttctttaga ctataatata cctataaatt
    11341 tgttttatat atgggggaga tttacatgtt ggtagcaaaa gaaaaaagtt atgagttagt
    11401 ggacaattgt cagcaggatg agtataacat tgtattaaga gcaagtgaag gggataaaat
    11461 agcactagag tacattatta ctaaatacag aaattttgta aaggcaaaag caaagtcata
    11521 ttttttaatt ggagcagata aagaagatat aatacaagaa ggaatgatag gcctttataa
    11581 ggcagttaga gattttgatg gaagtaagac caattcattt aaatgttttg cggagatatg
    11641 tataacaaga caaataataa ctgctataaa aactgcaaca agacaaaaac atataccttt
    11701 aaattcatat gtttctttga ataaaccaat atatgatgaa gagtcagaca gaacactttt
    11761 agatattata gctacaagta tagtaacaga tccagaagag cttataatta gtaaagaaga
    11821 gttaaaaaat atagaatcaa aaatgaacga attgttaagt gacttggaat tagaagtact
    11881 agaattatat ttaaatggaa agtcatacca gtttatagcg gacaagctta aaagagatgt
    11941 aaagtcaata gataacgctt tacaaagggt taagagaaaa ttagaaaaac atcttgaaaa
    12001 cagaaatgat taatagtaaa atacttacca taagcttatt gtgagcaaaa ttaatttata
    12061 tcgaaagata agggaggaaa ttcaaaatgg ctaaagctaa atacgaaaga acaaaacctc
    12121 atgttaatat agggacaata ggacacgtag accacggtaa aactacatta acagcagcaa
    12181 taacaaaaac attatatgac agatatcaat taggagaagc agtagatttc gcaaacatag
    12241 ataaagctcc agaagaaaga gaaagaggaa tcacaatatc aacagcacac gttgagtatg
    12301 aaacaccaaa tagacactat gcacacgttg actgcccagg acatgctgac tacgttaaga
    12361 acatgataac aggagcagca caaatggacg gagcaatatt agtttgttca gcaacagatg
    12421 gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga gtaccatata
    12481 tagtagtatt cttaaacaaa tgtgacatgg tagatgatga agagttatta gagttagtag
    12541 agatggaagt aagagattta ttaacagaat atgatttccc aggagatgac actccaatag
    12601 taagaggttc agcattaatg gcattagaag atccaaagag tgagtgggga gataagatag
    12661 tagaattatt cgagcaaata gatgagtata taccagctcc agagagagat acagataaac
    12721 cattcttaat gccagtagag gacgtattct caatcacagg aagaggaaca gttgcaacag
    12781 gaagagtgga aagaggagta ctaaaagtac aagacgaagt agaattagta ggattaacag
    12841 aagcaccaag aaaagtagta gtaacaggag tagagatgtt cagaaaatta ttagaccaag
    12901 cacaagcagg ggataatata ggagcattat taagaggagt acaaagaaac gagatagaaa
    12961 gaggacaagt actagcaaag actggatcag taaaggcaca cacaaagttt acagcagaag
    13021 tatatgtact taaaaaagaa gagggtggaa gacatacacc attctttgat ggatatagac
    13081 cacaattcta tttcagaaca acagacgtaa caggagcttg taagttacca gaaggaatag
    13141 agatggtaat gcctggagat aacgtaacaa tggaagtaga cttaataaac tcaatagttg
    13201 tagaagaggg attaagattc tcaataagag aaggtggaag aacagtagct tcaggagttg
    13261 ttgctacaat aatagagtaa ttctttacta aactgatgga ttaaaaggaa taacaaaaag
    13321 aggggttatg ctcctctttt tttaacatag aaatacaaaa acattgacac agcataggat
    13381 tttgtgataa cttatacaag gtaatgatta taggaagtta ataatatctt gaatactgta
    13441 gatttttaat aaatacatat tttaaagata ttatattcac atatattaac aatatatatt
    13501 attgtattac aaagttggag gtgtatcaga tgagagttaa agtaacttta gcatgtacag
    13561 agtgtaagca aagaaactac aacactacta agaataagaa aaacaatcca gacagaatag
    13621 aattacaaaa atattgtaga ttctgtaaaa agcatacaac tcataaagaa acaaaatagt
    13681 tttttataag gatgtgagta tggaatggct gcccaaaaaa atgacggtgc aaaaactaaa
    13741 aaaaggttta gtttgtttgg atacttaaaa gaaactaaac aagaattaaa aagagttaca
    13801 tggccgacaa aaaaggaact gtttaaaaac acaagtatag ttttaactgt agttatttca
    13861 tgcactatat tagtatgggg tatagacact atattatctg gtgcactagc gttattacta
    13921 aaatagacga aaagaggtaa gttaaatgtc agaattacaa gaagcaagtt ggtatgtagt
    13981 tcatacctat tcgggacatg aaaataaagt taaagctaca attgagaaag cagttaaaac
    14041 aagaggtatg gaagactgca taagacaagt agttgttcca acagaagaag tagtggaaac
    14101 tactaaaact ggaaaagaaa aaactagaca acgtaaagtt tatccaagtt atgttttggt
    14161 taaaatgata ataactgatg aatcttggta tgtagtaaga aatactaagg gagttacagg
    14221 tttcgttgga cctggttcaa aaccagtgcc attaagtgaa gacgaagtca aagctatggg
    14281 gatagacaca acagacccta aagtagtaaa tagcgatatt gattttgaaa taggagatac
    14341 agtaaaagta tctcaaggac catttagtgg acaaataggt aacatagaag aaattgactt
    14401 agaaaataga gaagtcaaag tatgcataaa tgcatttggt aaaaggactc tatttgtaat
    14461 agagcttgaa ggtatagaaa aaatctaata ctatattata gaaaaatctg aggaggtgcg
    14521 ctcaaaatgg ctaaaaaagt tataggtcaa ataaaattac aaatacctgc aggaaaggct
    14581 actccagcgc caccagttgg accagcatta ggacaacatg gtgttaatat aatgggattc
    14641 acaaaagaat ttaatgctaa aactgcagat caagctggaa tgataatacc agttgttata
    14701 actgtatatc aagatagatc attcagtttt ataacaaaaa ctccaccagc tgcagtttta
    14761 attaaaaaag cattaaactt aaaatcaggt tctggagaac caaacaagaa aaaagttgct
    14821 aaaatgactt cagctcaagt aagagaaata gctgaattaa agatgcctga cttaaatgct
    14881 gcatcagtag aagctgctat gagtatgata gctggtactg ctagaagcat gggtgttgta
    14941 atcgaagact aattaaaaga tacacttttt tttataaagt gggaggctag aagccgaaat
    15001 taaccacgag gaggaaaata aaatggctaa aaaaggtaaa agatatgcag gtgcattaca
    15061 aaaagtagat agaactaaat tttatgatgc atcagaagca ttaacattag tttcagatat
    15121 agctggagct aaatttgatg agacagtaga agcacacatt aaattaggtg ttgactcaag
    15181 acatgctgac caacaagtaa gaggtgcagt tgtattacct catggaactg gtaaaacaaa
    15241 aagagtttta gtttttgcta aaggtgaaaa agctaaagaa gctgaacaag ctggtgctga
    15301 ctttgtagga gctgaagaat tagttcaaaa aatacaaggt gaaaactggt ttgactttga
    15361 tatagtagtt gctactccag atatgatggg tgtagtaggt agattaggta gagtattagg
    15421 acctaaaggt ttaatgccaa accctaaatc aggaacagtt acatttgatg tagctaaagc
    15481 tatagatgaa ataaaagctg gtaaagttga atacagatta gacaaaacta acataataca
    15541 cgttccagta ggaaaagtat catttggtgg agaaaaatta actgaaaact ttactgcatt
    15601 aatggatgct ataataaaag ctaaaccagc tgctgctaaa ggacaatatt taagaagtat
    15661 aacagtagct tcttcaatgg gacctggagt taaaataaac ccagcaagaa cagctgaata
    15721 ataaaactgt tgacacaggt tgacaaaaat gttattatat cacttgtcaa tgtaaaaaag
    15781 aatagatttg ccgtagatag tgggtgcgag agcttaatat cccgccgagg ttttagataa
    15841 attgagatta ctaaagcttt tgatgtctac ttttggcaac aaaagctttt tataatgttg
    15901 cggtaaatac taagcctatt tggtgaagga ggtgcacatc tatatgagaa aagctataga
    15961 aattaaatca gaagttgttt ctgagatagt tgaaaagtta caaaaatctt ctgctgctgt
    16021 tgttgtagat tacaaaggat taacagttga agaagtaact gagttaagaa aacaaatgag
    16081 agaagctggt gtagattaca aagtatacaa aaatacttta gttagaagag cagctaaaga
    16141 agttggtata gagcaattta atgatgaatt attagttgga actaatgcca tagcatttgg
    16201 atatgatgat ccagttgctc cagctagaat attaaaagga ttcatggatt ctcatccaaa
    16261 aatgaaatta aagatgggta ttgtggaagg tgcattctat gatgaatcta agattgttga
    16321 aatggctaac ataccatcaa gagaagttct tatcgctaaa ttacttggta gcttaaaagc
    16381 tccagtatca aacttcgcat acttaataga tgcaatagcg aaaaaggcag aaggtcaaga
    16441 agaagcttaa ttgcttaact aaaaaattat aaatattaat ttgaaataaa attcggaggt
    16501 gctaaaaatg acaatagaac aaatattaga agctatagaa aatatgaaag ttttagaatt
    16561 aaatgaatta gttaaagctg ctgaagaaaa attcggtgta tcagcttcag ctccagtaat
    16621 ggtagctggt gcagctgctg gtggaccagc tgctgaagaa aaaactgagt ttgatgtagt
    16681 attaactgac gttggttctt caaaagttgg agttataaaa gcagttagag aaataacagg
    16741 attaggatta aaagaagcta aagaagtagt tgacaatgct cctaagacag ttaaagaagg
    16801 agcttctaaa gaagaagctg atcaaataaa agagaaatta gaagctgctg gagctaaagt
    16861 agaagttaag tagtttcttt aaaaagatac ccattgggta tcttttttta tttataaaaa
    16921 actataaaga aagtatttgg aattataaag aaagtatttt tataaaataa aaaaaattta
    16981 tataatagta ctaaattaag ggtatcatat atgatgtaat ggtattttaa tttaacatta
    17041 ttatcaaatt aaaatataaa caattgttgt aatgtctata tataaagtat ataattaaac
    17101 taaaatgtaa aattttacat tttagtttaa aaaaatacac gagtgttttg taaataaaat
    17161 gtaataaata attaattaac aaatatgaat tttaaaataa gttagatatt tagaaaaatc
    17221 gaaatttgta ggatttaaag gacatattca aggaggagtt aataatggaa aaattacaag
    17281 gaaaaattgc agtagttact gcagcaacaa aaggtattgg attagcatca gcagagatat
    17341 tagcaaaaaa tggagcaact gtgtacttag cagctcgttc agaagaatta gctaatgaag
    17401 ttataaataa aataagtgca gaaggtggtt gtgctaagtt tgtttacttt aatgctcgtg
    17461 aagaagagac ctttacctca atgatagaag aggtagttaa aaaagaaggt aagatagata
    17521 tacttgtaaa caattttggt tcaacaaacc cttctcttga caaagacctt gtaactgggg
    17581 atacagataa tttctttgac acagtaaata ctaatttaaa aagtgtgtat ttaccatgta
    17641 aagcagcaat tcctcatatg ataaagaatg gaaaaggaag tatagtaaat atatcaagta
    17701 taggttcagt attacctgat ttatctagaa tagcttactg tgtatcaaaa gcagcaatca
    17761 actcattaac tcaaaacata gctacacaat atgctaaaga caatgttaga tgtaatgctg
    17821 tacttccagg gcttattgca actaaagcag cattagacaa tatgtctcca gagttcataa
    17881 aagaattttt aaagcatgtt cctttaagtc gtatagggga accagatgat atagcaaaag
    17941 ctgttctatt ctatgctagt gatgactcat catttataac aggggattta cttgaagttg
    18001 caggaggatt tggtttacct actccacaat ttgcagataa tatattagga taattaaatt
    18061 actaatatgc aatgaaagct tatgcattag ttatagtaga aatgatattt ttataaaggc
    18121 catagatata atttatattg gaaaggtttg aggttcaaaa ttccaagtta aatttattta
    18181 tggtctttat tttgtatatt ttaataaatt gtatatattt gtataaatgt aattatttac
    18241 aatcatttta atatggaatt ataattaaat aggggggatg gtacttgtat aaaatttttt
    18301 atagaaaatt agacctataa atgaaaaata tggattttat attggaaaat tgtacataat
    18361 acataatata tgcttaaaat aatttacaaa aaatcaaaaa taatgcatag tttacatata
    18421 agataaaaaa tatattgaaa aatacttgac attatgctaa aattattaga tgcgttagaa
    18481 tgaactattg ttttattttt aattattgag aggtgaaaag tgaatgccac atcctgtcac
    18541 gataggtaaa agaactagaa tgagcttttc taaaattaag gaaatagctg atgtgccaaa
    18601 tcttatagaa attcaagtag attcctatga gtggttttta aaagaaggtt taaaagaagt
    18661 gtttgatgat atttcaccaa tagaagatta tactggtaat cttatattgg aatttgtcga
    18721 ctattctcta gatgataaac caaagtacga tatagaagag tgtaaagaga gagatgctac
    18781 atattgtgca cctctaaagg ttaaggtaag acttataaat aaagaaacag gtgagataaa
    18841 agaacaagaa gtgtttatgg gtgacttccc tttaatgaca gaaagaggaa ctttcgttat
    18901 aaatggagca gaaagagtta tagtaagtca attagttaga tctcctggtg tttactatgc
    18961 ggaagaaaga gataaaacag gtaagagatt gatttcatct actgtaattc ctaatagagg
    19021 agcatggtta gaatatgaaa cagattctaa tgatgtaata tctgtaagag ttgatagaac
    19081 aagaaaacaa ccagttacag ttttacttag agctttagga ataggaacag atgcagagat
    19141 aattgacctt ttaggagaag atgagagatt atctgctaca ttagaaaaag ataatactaa
    19201 gacagtagaa gaaggtcttg tagagatata caaaaaatta agaccaggtg aacctcctac
    19261 tgtagaaagt gcatcatctt tattaaatgc tttattcttt gacccaaaaa gatatgacct
    19321 agcaaaagtt ggtagatata aattcaataa gaaacttgct ttatgttata gaataatgaa
    19381 taaaatttca gcagaagaca taataaatcc agaaactgga gaagtatttg taaaagctgg
    19441 agaaaaaata agctatgatt tggctaaagc catacaaaat gcaggtataa atgtagtaaa
    19501 tctactaatg gatgatgata aaaaagttag agttatagga aataattttg tggatattaa
    19561 gtctcacatt gattttgaca tagatgattt aaacataaaa gaaaaagtac attatccaac
    19621 tttaaaagaa attttagatg gatatagtga tgaagaagaa ataaaagagg ctataaaatc
    19681 aagaattaaa gaacttatac caaaacatat attattagat gatataatag cttcaataag
    19741 ttatgagttc aatatattct ataatatagg aaatattgat gatatagatc acctaggaaa
    19801 cagaagaata agatctgtag gtgaattgtt acaaaatcaa gttagaattg gtctttcaag
    19861 aatggaaaga gttataaaag aaagaatgac ggttcaagat atggaagcta taacgcctca
    19921 agcgttagtt aacataagac cagtttcagc tgcaataaaa gaattttttg gtagttctca
    19981 gttatctcag ttcatggatc aaacaaatcc tctatctgag ttaacgcata agagaagatt
    20041 atcagccctt gggcctggag gtctttcaag agaaagagct ggattcgaag tgcgtgacgt
    20101 tcaccattca cactatggta gaatgtgtcc aatagagact ccagagggac caaatatcgg
    20161 tcttataaac tctctaggaa cttatgcaaa aataaatgaa tttggattta tagaatcacc
    20221 atatagaaaa tttgataaag aaacatcaac agttactgat gaaatacatt atttaactgc
    20281 tgacgaagaa gatttattcg taagagctca ggcaaatgag ccattaacag aagatggtaa
    20341 attcgtaaac catagagttg tttgtagaac tgtaaatggt gctgttgaaa tggttcctga
    20401 aagtagagta gattacatgg atatatctcc taagcaggtt gtatctgttg ccactgctat
    20461 gatacctttc ttagaaaatg atgatgccaa ccgtgccctt atgggagcga acatgcagcg
    20521 tcaggcagtg cctctagtta gaagagaagc accaattata ggaactggta tagaatacag
    20581 agcagcaaaa gactctggag cagttgttgt tgctagaaat tctggtatag cagaaagagt
    20641 aacagcagat gaaataatta taaaaagaga agatggaaac agagatagat ataatctact
    20701 taagtttaaa cgttcaaact caggtacttg tataaatcaa acacctataa taaataaggg
    20761 tgaccagata ataaaaggtg atgttatagc agatggtcca gcaactgatt taggagaagt
    20821 tgcacttgga agaaactgtc ttatagcatt catgacttgg gaaggttata actacgagga
    20881 tgctatatta ataaatgaaa gattagttaa agaagataga ttatcaacaa ttcatataga
    20941 agaatatgaa tgtgaagcta gagatacaaa actagggcca gaggaaataa ctagagatat
    21001 acctaatgtt ggagatagtg caattaagaa cttagatgac agaggtataa taagaatagg
    21061 tgcagaagta gactcaggtg atatattagt tggtaaagta actccaaaag gagaaactga
    21121 acttactgct gaggaaagat tacttcgtgc aatatttgga gaaaaggcta gagaagttag
    21181 agatacttca cttaaagtgc ctcatggtga atctggtata atagttgatg taaaagtatt
    21241 tactagagaa aatggagatg atttatctcc aggagtaaat gaattagtaa gatgctatat
    21301 agctaaaaag agaaaaataa aagttggaga taaaatggct ggtcgtcatg gtaataaagg
    21361 ggttatctca agagtattac ctgaagaaga tatgccattc atggaaaatg gaacaccact
    21421 agacataata cttaatccac aaggtatacc atcacgtatg aacattggtc aggttttaga
    21481 agttcattta ggacttgctg ctaagacatt aggatggtat gtagcaacat cagtattcga
    21541 tggtgctaat gagtatgaca taatggatgc tcttgaagaa gctgggtatc ctagagatgg
    21601 taaattaact ttatatgatg gtagaacagg tgagtctttt gacaacagaa taactgttgg
    21661 atatatgtac tacttgaaac tacatcactt agtagatgaa aaactacatg caagaagtac
    21721 tggaccatac tcattagtta ctcaacagcc actaggtggt aaagcacaat ttggtggaca
    21781 aagatttgga gagatggagg tttgggctct ggaagcatat ggagctgctc acatacttca
    21841 agaaatactt actgttaagt ctgatgacgt tgtaggtcgt gttagaacat atgaagctat
    21901 agttaaaggt gaaaatatac ctgaaccagg aatcccagaa tcgtttaagg ttcttataaa
    21961 agaacttcaa agtttatgct tagatgtaaa agtattgaca gatgaagacc aagaaataga
    22021 agtaagagaa tctgtggatg aagatgatac aataggtgaa tttgagctag atgttgtaaa
    22081 tcatatggga gaagttgaag aaagtaatat aatagaagaa attgaagatg attttgcaga
    22141 aaatgcagaa gatgaggata tagagaattt agaagaattc actgaagatg atttatttga
    22201 agaagaaata gattttgata gtgatgactt tgatatgtaa atttaattaa tgtaaatctc
    22261 aaatagaagg gagagaactc cttgtttgaa ttaaacaatt tcgagtcgat aaaaatagca
    22321 ttggcttctc cagaaaaaat aagacaatgg tctaggggag aagttaaaaa gccagaaact
    22381 ataaattacc gtactttaaa accagaaaaa gatggtcttt tctgtgaaag aatatttgga
    22441 ccacaaaaag actgggagtg ccactgtggt aaatatagaa gagttagata taaaggtgta
    22501 gtttgtgata gatgtggagt agaagtaact aaatcaaaag taagaagaga gagaatggga
    22561 catatagagc tagctgctcc tatgtctcac atctggtact tcaaaggtat accaagtaga
    22621 atgggacttt tacttgatat gtcaccaaga tcgttagaaa aaatattata ctttgcctca
    22681 tatgtggtag ttgatccagg agagactgga ttaaacgaaa aacaattgct tacagaaaaa
    22741 gaatacagaa ctgctcttga aaagtatgga tatactttta ctgtaggaat gggtgctgaa
    22801 gctgtaaaga cattactaca aaatatagat ttagagcaac aaagtaaaga cctaagagcg
    22861 gagttaaaag atagtacagg gcaaaagaaa gttagaacaa taagaagatt agaagttgta
    22921 gaggcattta aaaagtctgg aaataaacca gaatggatga ttttagatgc aataccagta
    22981 ataccaccag atttaagacc aatggtacaa cttgatggtg gaagatttgc gacttcagac
    23041 ctaaatgatt tatatagaag agttataaat agaaacaata gacttaaaag attattagag
    23101 cttggagctc cagatataat tgtaagaaat gaaaaaagaa tgcttcaaga agctgttgat
    23161 gcattgatag ataatggtag aagaggtaga cctgtaacag gacctggaaa tagaccactt
    23221 aaatctttat cagatatgtt aaaaggtaag caaggtcgtt tccgtcaaaa cttacttggt
    23281 aagcgtgttg actactcagg acgttctgtt atagttgttg gaccagaact taaattttat
    23341 caatgtggac ttccaaagaa gatggcatta gaattattca agccatttgt tatggataaa
    23401 ttagttaaag aaggttatgc acataatata aaaagtgcga aatctatagt agaaaaagtt
    23461 aagccagaag tttgggatgt tttagaagat gttataaaaa gtcatccagt tcttcttaac
    23521 cgtgcaccga ctctacatag attaggtata caagcatttg aaccaatctt agttgaaggt
    23581 aaggctataa aactacatcc tcttgtatgt acagcttata atgcagactt tgatggtgac
    23641 caaatggcag ttcatgtacc tttatcagta gaagcacaag cagaagcaag attcttaatg
    23701 ctttctgtaa ataatatact tgctcctaaa gatggttcac ctataactac tccatctcag
    23761 gatatggttt taggttgcta ttatctaaca atagaagccc aagatggagc taaaggaaca
    23821 ggtatggtat ttaaagactt taatgaacta ttacttgctt attacaataa atcagttcat
    23881 ctacatgcat tagtgaaact taaggtaaca cttgaagatg gaagaagttc attggttgaa
    23941 agtactgttg gtagatttat atttaatgaa aatatacctc aagacttagg ttttgtagat
    24001 agaaaagaaa atccatttgc acttgaagtt gatttcttag cagataaaaa atctcttggt
    24061 aaaataatag ataaatgctt tagaaaacac ggaaatacag aaacagctga attactagat
    24121 tatataaaag ctctaggatt taaatattct acattaggtg gcataacagt tgctgttgat
    24181 gatatgagtg tcccagaaga aaagaaagta tttatagctg aggcagaagc taaagttgat
    24241 aagtatgaaa aagcatacag aagaggtcta atctctgatg aagaaagata tgaaaaagtt
    24301 atagagacat ggacagaaac aactgataaa gttactgatg ctcttatggg tggactagat
    24361 agattaaata atatatatat aatggcacat tcaggagcca gaggttctaa aaaccaaatt
    24421 agacagctgg caggtatgcg tggtcttatg gcaaatgcat ctggtaaaac agttgagata
    24481 ccagttaaat ctaatttccg tgaaggttta tcagtactag aatactttac atcttcacat
    24541 ggtgccagaa aaggtcttgc cgatacagct atacgtacag ctgaatctgg atatttaaca
    24601 agaagacttg ttgatgtaag tcaagatgtt attgtaaggg aaatagattg tggaacagaa
    24661 gatactacag agatttatgc tataaaagaa ggaaatgaag ttatagaaga gatatatgat
    24721 agaattgtag gaagatatac tatagaccct atattaaatc ctgaaactgg agaagttata
    24781 gttgaggctg attcaatgat acaagaagat gaagcagaaa ctatagtagc tttaggaatt
    24841 gaaaaaatta ggataagaac agttcttaac tgtaaaacta atcatggagt ttgttctaag
    24901 tgttatggta gaaacttggc aacaggaaaa gaagttaata taggtgaagc agttggtata
    24961 atagcagctc aatctatcgg tgagccgggt actcagctta caatgcgtac attccatact
    25021 ggaggagttg caggagctga tataactcaa ggtctaccaa gggttgaaga attatttgaa
    25081 gctagaaaac caaaaggatt agctgtaata actgaagtat ctggtagagt tgaaattgat
    25141 gaaacaggaa agagaaaaga agtaaatgta ataccagaag aaggcgagac tcaaacatat
    25201 gtaattccat atggttctag attaaaagtt aagcaaggtc aaatgctaga ggctggagac
    25261 cctctaacac aaggatttat aaatcctcat gatatagtaa gagtaaatgg tgttaaggga
    25321 gttcaagaat atatagttaa agaagttcaa agagtgtata gacttcaagg ggttgatgtt
    25381 aatgataagc atatagaagt tatagtaaga caaatgctat ctaaagtaaa agttgaagac
    25441 ccaggagata cagatttatt gccaggtgga tatgaagatg tattaacatt caatgaatgt
    25501 aataaagatg ctatagataa aggtctaaga ccagcagttg ctaaaagagt tttacttggt
    25561 ataactaaag catctcttgc aactgattca ttcttatcag cagcttcttt ccaagaaaca
    25621 acaagagtat taacagaggc agcaataaaa ggtaaagaag accacttaat aggacttaaa
    25681 gaaaatgtta tattaggtaa gttaatacca gcaggaacag gaatgaagaa atatagaaat
    25741 atagctgttg aaaaaattga agattaatag catatagagt aaagaaatct tgacaaccat
    25801 tatttgtggt gttaaaatag taaagtgtgt tgtttaaaag tacactaagg gcttgaacaa
    25861 gttcttagtg acttttagat ggcaataaat gagaactaaa aagcatcaag ctagaaaagt
    25921 gctaatatca catatttatg tgttatagac attaaattgt aagtaacgaa tgttacaatc
    25981 agtcagtatg aatattcata ttgatgtagt tggcggtcac tgataagatc gaaaatttgg
    26041 aaggaggtgc agatgatgcc aacaattaac caattagttc gtaaaagtag aaaagcgcta
    26101 gagaagaaat ctactgctcc agcattacaa aaaggttaca actctttaaa taagaaagta
    26161 actgacgcta gtgccccaca aaaaagaggg gtttgtactt cagtaaaaac agttactcct
    26221 aagaagccta actcagcttt aagaaaagtt gccagagtta gattaactaa tggtatagaa
    26281 gtttctgctt atataccagg agaaggacac aacttacaag agcatagtgt tgttcttata
    26341 agaggaggaa gagtaaaaga ccttccaggg gttagatacc atatactaag aggtactcta
    26401 gatacagcag gtgttgataa gagaagacaa tcaagatcta aatacggtgc gaagagacct
    26461 aaagaagcta aaaagtaaaa acaattaatg attgattata gttcggtgct ttaaatatat
    26521 atatatttac ggcatcacgg cattaaatga tttatgtcga gtacctatga tttaagttaa
    26581 ataattaagg agggaagcga aatgccaaga aaaggtaata ttccaaaaag agaagtttta
    26641 ccagatccta tgtatgggag taaggtagtt actaaattaa taaataatct aatggtagac
    26701 ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt tcgcaattgt tgctgaaaag
    26761 acaggagaag aagctttaga agtatttaat aaagcaatgg ataatataat gcctgtttta
    26821 gaagtaaaag caagaagagt tggtggggca aactaccaag tgccaatcga agttagacct
    26881 gaaagaagac aaacattagg tttaagatgg ctagtaaaat atactagagc tcgtggagaa
    26941 aaaggaatgg ttgaaaagct agctaaagag ataatggacg cagctaacaa cactggagct
    27001 tcagttaaga aaaaagaaga tactcataaa atggcagaag ctaataaagc atttgctcat
    27061 tacagatggt aggatatata gatatcttat aaaattgagg atatcataag gaggataacc
    27121 atggctagaa agtttccttt ggaaagaact aggaatatag gaatcatggc tcatatagat
    27181 gcaggaaaaa ctactactac agaaagaatc ctgttttata ctgggcaaac tcataaaata
    27241 ggagaaactc atgaaggagc ttctcaaatg gactggatgg agcaagaaaa ggaaagaggt
    27301 ataacaataa cttctgccgc tactactgct tcatggaaag accatagaat aaacataata
    27361 gatactcctg gacacgtgga cttcacagtt gaggttgaaa gatctctaag agttcttgac
    27421 ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc ctcaatcaga aaatgtatgg
    27481 agacaagctg aaacttatgg agtacctaga atagcttttg taaataaaat ggatatatta
    27541 ggtgcagatt tctacaatgt tgtaagcatg atgaaatcta gattaaattc taatgcagtt
    27601 cctatgcaat taccaatagg taaagaagat tcatttatag gtataataga cttattaaaa
    27661 atggatgctg ttatatacaa agacgaccta ggggttgaaa tggaagaaac agatatacca
    27721 gaagatatga aagaattagc tgctgagtgg agagaaaaat tagttgaatc agtagctgaa
    27781 actgatgaag aattaatgat gaaatacctt gaaggtgaag aacttactat agatgaatta
    27841 aaagttgcta taagaaaagc tactatagct tgtgaaatga acccagtttt ctgtggtaca
    27901 gcttatagaa acaaaggtgt tcagttagta atagatgcag ttttagatta cttaccagcg
    27961 ccaactgata taccagctat aaaaggtata ttagcagatg gagaagaagc tgaaagacat
    28021 tcttctgatg aagagccatt ctctgcatta gcattcaaaa taatgactga cccattcgtt
    28081 ggaaaactag cgttcttcag agtttattct ggaacattag aatcaggttc ttatgtttta
    28141 aatgcaacta agaataagag agaaagaatt ggtcgtatat tacaaatgca tgctaataca
    28201 agagaagaaa taactaaagt gtatgctgga gatatagcag cagcagtagg attaaaagat
    28261 actactactg gagacacatt atgtgaccct gctaatccaa taatacttga atctatggaa
    28321 ttccctgagc cagttatatc tgttgctata gaaccaagtt ctaaagcagc tcaagaaaaa
    28381 atgggtatag ctcttcaaaa attagctgaa gaagacccaa ctttcacagt taagactgac
    28441 caagaaacag gacaaactat aatatctggt atgggtgaat tacacttaga gataatagta
    28501 gatagattat taagagaatt taaagtagaa gctaaagttg gtgcaccaca agttgcatat
    28561 agagagacta taactcaacc agttgatgtt gaatacaagt actctaagca atcaggtggt
    28621 agaggacaat acggtcacgt taagattaga gttgctcctc aagagccagg tgaaggatac
    28681 aaattcacaa acaaaactgt tggtggatca gtaccaaaag aatatgttgg tccagttgac
    28741 atgggtatac aaggagctat gcaatctggt atagttgctg gatatccagt tgtagacgtt
    28801 gctgttgagt tatatgatgg ttcttaccat gaggttgact cttctgagat ggcgtttaag
    28861 atggctggtt caatggcatt caaagatgct atgaagaaag gtaatgcagt tcttttagag
    28921 ccttacttca aagtagaagt tgttactcca gaagattaca tgggagatgt tatgggtgac
    28981 ttaaactcaa gaagaggatt aatccaaggt atggaagcta gaagtggtgc acaagttata
    29041 aatgcattcg ttccactttc agaaatgttt ggatactcaa cagacctaag atcttcaact
    29101 caaggtcgtg caacttacac aatgatattc gatcactatg agcaagttcc agcttcagtt
    29161 gctaagaaaa tagctgaagg aaaataattt aaaaataatt aataatataa atataaaaaa
    29221 taatcagcta gatgagagat atctctcatc tagcacaaga t
//
LOCUS       DAGSHT010000028        29132 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.029, whole genome shotgun sequence.
ACCESSION   DAGSHT010000028 DAGSHT010000000
VERSION     DAGSHT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.029"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..314)
                     /locus_tag="MJB72_001163"
     CDS             complement(<1..314)
                     /locus_tag="MJB72_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262288.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     EL"
     gene            complement(568..2268)
                     /locus_tag="MJB72_001164"
     CDS             complement(568..2268)
                     /locus_tag="MJB72_001164"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBY8262289.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(2527..2940)
                     /locus_tag="MJB72_001165"
     CDS             complement(2527..2940)
                     /locus_tag="MJB72_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBY8262290.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(3008..5386)
                     /locus_tag="MJB72_001166"
     CDS             complement(3008..5386)
                     /locus_tag="MJB72_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBY8262291.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            5705..7156
                     /locus_tag="MJB72_001167"
     CDS             5705..7156
                     /locus_tag="MJB72_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBY8262292.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GNMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(7360..9873)
                     /locus_tag="MJB72_001168"
     CDS             complement(7360..9873)
                     /locus_tag="MJB72_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY8262293.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(10096..10917)
                     /locus_tag="MJB72_001169"
     CDS             complement(10096..10917)
                     /locus_tag="MJB72_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY8262294.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(11435..11911)
                     /locus_tag="MJB72_001170"
     CDS             complement(11435..11911)
                     /locus_tag="MJB72_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBY8262295.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(11946..12524)
                     /locus_tag="MJB72_001171"
     CDS             complement(11946..12524)
                     /locus_tag="MJB72_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBY8262296.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(12628..15228)
                     /locus_tag="MJB72_001172"
     CDS             complement(12628..15228)
                     /locus_tag="MJB72_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBY8262297.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSKIDTLDK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            complement(15798..16481)
                     /locus_tag="MJB72_001173"
     CDS             complement(15798..16481)
                     /locus_tag="MJB72_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBY8262298.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(16867..17457)
                     /gene="zapA"
                     /locus_tag="MJB72_001174"
     CDS             complement(16867..17457)
                     /gene="zapA"
                     /locus_tag="MJB72_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBY8262299.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(17482..19875)
                     /locus_tag="MJB72_001175"
     CDS             complement(17482..19875)
                     /locus_tag="MJB72_001175"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBY8262300.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(19892..20911)
                     /gene="pheS"
                     /locus_tag="MJB72_001176"
     CDS             complement(19892..20911)
                     /gene="pheS"
                     /locus_tag="MJB72_001176"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBY8262301.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(20961..21195)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21539..22336)
                     /locus_tag="MJB72_001177"
     CDS             complement(21539..22336)
                     /locus_tag="MJB72_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBY8262302.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     gene            complement(22525..23196)
                     /locus_tag="MJB72_001178"
     CDS             complement(22525..23196)
                     /locus_tag="MJB72_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBY8262303.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            complement(23483..24859)
                     /locus_tag="MJB72_001179"
     CDS             complement(23483..24859)
                     /locus_tag="MJB72_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBY8262304.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            25288..27030
                     /locus_tag="MJB72_001180"
     CDS             25288..27030
                     /locus_tag="MJB72_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBY8262305.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGNDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(27194..27388)
                     /locus_tag="MJB72_001181"
     CDS             complement(27194..27388)
                     /locus_tag="MJB72_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262306.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(27385..27966)
                     /locus_tag="MJB72_001182"
     CDS             complement(27385..27966)
                     /locus_tag="MJB72_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8262307.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            28134..28952
                     /locus_tag="MJB72_001183"
     CDS             28134..28952
                     /locus_tag="MJB72_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8262308.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
BASE COUNT         9328 a         5130 c         3276 g        11398 t
ORIGIN      
        1 aaaagttctt cttttttaaa ataaataaca tactttttta ttagtttaat aacctcatct
       61 tcactttttt gaatattagt atttgataaa attagtggta ataaaatatt taaaaaattg
      121 atatttccta ttttttctcc ataaaatcta tagtaaataa gttcatctct cagtctattt
      181 tcgtctatat acacacatct ttgcgtttta tcatctatta aggattttgt atttattata
      241 gttaaatctt taagagccct acttgcacta ctaaaagaag atgtgtagac tttttttagt
      301 aattcgtcat tcataaatat atcctttctg aaaaattgaa tgtaaataaa ttgttattaa
      361 aaaaaaagag acagcccgtt taagaagctg tcatatatag ggggatagta gtgtacaaaa
      421 atcattgtaa tctactatct atatattatt atacgatact acttttttat atgtaaagca
      481 tcagttttcg acatccctat attttacagt tatacaaatt gcgtactaag ttcaatttta
      541 ttctttttat ttcaacattt tatcatgcta cattctttca ggagcgtgca ttcccaacaa
      601 tttcagagaa ttttctaaag tttgttttgt tgctaaaact aatgcaagtc ttgcttttct
      661 tatttccaaa ttttctacta aaattggatt gtcatgataa aatttattaa acgcttgagc
      721 taaatctagc ataaatctag tcactatatg tggctcattt ttctttagag caagtaaaat
      781 attcttattg aatgattcta taactctaag aacttctgca ctatcttcat cagctaatag
      841 actataatct atatcagaag tcacttctac ttctgctttt cttaaaactg cacagcatct
      901 agcatgtgta tactgaacat atggtccagt ctcaccatca aaactaagcg ttctttccca
      961 agagaaggta tagtctttaa ttctactatt agataactct tggaatacta ctgccccaac
     1021 acctacttgc ttagatattt catccacatt tttagcattt gggtttttag ctagtataat
     1081 ttccttagtt ttatctatag cttgatttaa agcatcttct aaaaatacta ctcttccttt
     1141 tctagtcgac atagtacctt cttcaagtgc aaccatacca aatccaacat gaattaaatc
     1201 ttttgcccaa tcatatccca taagctcaat taccttaaac cattgttgga aatgtaagtt
     1261 ttgttgagaa ccaactacat atatacactt gtcaaaatca taagtttctt ttctatatat
     1321 tgcagcagct aaatctcttg tcatatataa cgttgaacca tcattcttag taataagtgc
     1381 tggtggcatt ttatactctt ctaaatcaac aattcttgct cctttagact cttttaaaag
     1441 atttttctct tctaatagtt cgataactct gttcatttta tcagaataaa aactctctcc
     1501 tgctaaagaa tcaaattcta tatctaaaag tttataaact ctgttaaact cttttaaact
     1561 ctcatttcta aaccattgcc ataattcttg tgcttcttcg tcaccatttt ctaatttatt
     1621 aaaccatgct cttgcttcat cttccatttc tgggtgttgt tcagcttcat catggaatct
     1681 tacgtataat tttagcaatt ctggaattgg attagactct actacttctt tttctcccca
     1741 ctttttaaag gccactatta acttaccaaa ctgagttcca tagtcaccta aatgatttat
     1801 tcttatagtc ttgtatcctt gcgaatcata tatcttatat aaagcattac ctataactgt
     1861 agttcttatg tgccctatat ggaaaggttt tgctatattt ggagaagaat attcaactat
     1921 tacagtttta tcttttccaa aatcactatg tccgtaattt tctttttcat ctaaaacttt
     1981 tttaattaca gtttctgcta attgagattt atttacaaag aaatttacat atccgcctaa
     2041 ttgtattact tttgttatct ctccacttgg ttctatactt tccgccaatt cacttgctat
     2101 catgtttgga gcttttctaa acactttggc taatttaaag catggaaaag catagtcccc
     2161 catatcctta tttggtggaa cttctataag tgcctctatc tcttctttcg ataaatcttc
     2221 tattttttct ttaaggcaat ttgatattgc caccttaaaa tcttgcattc tgttgctcct
     2281 ctctacattg ttcctggttt atattgtgct tatctcttta tgttaaatca aatttcgtgt
     2341 tttttcaagt atttcattac agtgtaaaac actttagtaa ttatgttttc tgtatatatt
     2401 aaaaatacta taattgcagt ttttaatcac actattattg attttttata agactttttt
     2461 ctattttatt gtttcttgta ctatccagtt gactttatat acttttttta atcttttatc
     2521 tattttctat aaatcttttt cactcttaac ttttattcct tcttcttcta atagccttgc
     2581 tgttatacct ttccctggta tctttttacc tgaaaaacta ccatcatata ttagattaca
     2641 tccacaagag gggctaccct cttttagtat tgcttcgcta catacatata aatttgctat
     2701 ttttagagtt tcattagccc ctcttacaaa gttttctgtt acatccttat tatctttagt
     2761 aattactttt ccatcaacta tctcagcagg tggtcttggt gtagatagac cacctaactg
     2821 ctctgggcat acaagtgtaa attctttgcc ttttaaatat tccttgactt tttcattatc
     2881 attattatcg ccactatatt tacaatttat tcctattaaa catgcagata ccaatatcat
     2941 aaaaatgtgc tcctatacaa attcttaata tattctcata caaattacta atgtattctt
     3001 atacaaatta ttttaattta acaattgtta ctcctgctcc accttctcca tattgaccac
     3061 ctctttgtga ttttacatgt ctatttcttc ttaaaatgtc ttgtagccct gcttttaata
     3121 ctcctgtacc tattccatgt attactgtta cagactctaa acctgccaca tatgcatcat
     3181 caagatactt ttctacttcc attatagctt cttctaggtt taaccctcta aggtcaactt
     3241 cattttttac actaccagat tttgacctta taatgtttct cgttgattta gttacatttg
     3301 ttgaaacatc tttgcgtgtt ttttgtaaag atttaaaagg tagagtcatc ttcattatac
     3361 caatttgcac tactgcttct tttctctttt tatctactga aactacacta ccattttgat
     3421 ttaaagttat taccttaact tcttcacctg gctttaagtc ttttatctcc ttatttgata
     3481 cctttggaac tatcatactt tttactgttg gctgaagact tcccatagag ctggtcaatt
     3541 cttttctaag ttcttctatc ttacgattct tttcttttga agctctttct tgttcaagac
     3601 ttctaagttc ttttattata atatctactt cttctttagc ttgtcttata attgaaaatg
     3661 cttctgattt agctttttca atcatcttat ctctttgact gaccaatttt tcaagttttt
     3721 cgtcatactc tactttcagt ttttcaatct cttcttttaa tctttcagct tcttctctat
     3781 cttctactgc tttgattcta ttcttttcaa cattctgtaa cacatcttct aaagctatgt
     3841 tttctgtatt tatatattct ttagcacgtg aaatcacata atcacttagc ccaagttttc
     3901 tagaaatctc aaacgcattt gattttccgg gaacacctat aagtaactta tatgttgggc
     3961 ttaaagtttc tatatcaaat tcaactgctg cgttttctac acctgattta gtaagagcat
     4021 aatttttaag ctcactatag tgagtggtag ctatacattt agctcctaca gaatttatat
     4081 cttctaatac tgctattgca agggcagctc cttcaacagg gtctgttccc gctcctaact
     4141 cgtcaaatat aactaaagag tctgctgtta cgttttgtaa tatagatact atattcgtca
     4201 tatgagatga aaatgttgat agactctgtt caatactctg ttcatctcct atatctgcaa
     4261 atacattatc atatacacac atactacttc cataatcagc aggtatatga agtccacttt
     4321 gtgtcataag tgcaaataga cctacagttt ttattgtaac agtcttacca cctgtgtttg
     4381 gacctgttat caccaatgta tggaagtctc ttcctaaata aattgtattt gctactacct
     4441 tctttttatc cagcagtgga tgtcttccat tttttatgtt taaatattta tcctcattta
     4501 aagttggttc tattcctctc atctgtatag aaagttttcc ctttgaaaat gcaaagtcca
     4561 atcttccaag tatctcttga ttagagatta aatcttcgct cacttctcca accatagcag
     4621 aaagctctga taaaattctt tctatttcct cttgctctcc tagtttcaac tgcctcaatt
     4681 cattgttcat ctctactata gtcattggct ctataaaaag tgtcgcacca gaagatgact
     4741 ggtcatgcac tataccagct acttgcgacc tatattcaga ttttacaggt actacaaatc
     4801 tatcacctct tagagaaatt attgcatctt gtaagtactt ttgataagta gttgaactta
     4861 ttattgaatt taattttgac ctaatggatt gatttttttg ggctattctt cttctgatat
     4921 ctctaagtgt acttgaagca ttgtcagata tttctacttc actaattata gcattgtata
     4981 tctggtcttc tatgtccttg tatacataca gagaatttga tagagattgt attattggat
     5041 aattaaaatc ttcttcatca ctactagata agctatttct taatcttcta gccactctta
     5101 aggtatctgc tatcataatc agacttcctg ggtcaagaga agcacctata taagctctct
     5161 ttaccttatc ttctatatca tgaatacctt ccaaccctac tgaacctctc tttataataa
     5221 ttgattgagc ttcactagtt tcttctagca tagatttcac ttctacaaaa tcagtatttg
     5281 gtactaaatt ttctatatat tttagaccta aagaagatga ggctttcttc tttaacaaat
     5341 ctattatttt attgtattct agaactctta acgatttctc attcattttc tatattcacc
     5401 atcctcgata ttttaacttt tttatttaat tttaaaaatg tatgttttca agcatttcca
     5461 ataaaaactg caagttttat ttctttttca tttctaaaat tattatattt tttctctttt
     5521 agatatattt tacacatatt ttgtattata ctatgtatgt agctatctat atattataca
     5581 taaacatgct ttatttgtaa tataatctgt taaaaaatct tctttaatat ttttaaagga
     5641 gtttatttta gcttataaaa aaaaatagct acttatatag ataaattaag gaggaacatg
     5701 agtcatggga aacgtattag aaggattaaa accagaaagt gtttttaaaa actttgagaa
     5761 aatatctcaa attcctagag gttctggaaa tgaaaaaggc ataagtgatt ttttacttag
     5821 ctttggtaaa aatttaggtc ttgaaactat tcaggatgaa agcttaaaca taataataag
     5881 aaagcctgct acaaaaggat atgaaaattg tccaggtgta gttcttcaag gacatatgga
     5941 tatggtttgc gaaaaagaaa agaatgttga acatgatttt cttaaagacc ctataaaatt
     6001 aagaattgat ggtaacatga tatatgctac tggaactact ctaggagctg acaatggtat
     6061 agctgttgct atgggtatgg ctatattaga agataatact ttagagcatc ctgcactaga
     6121 agttttagtt actgttaatg aagaagatgg tatgaatggt gcagatgcac tagaccctag
     6181 cttaattaaa ggtcaatata tcctaaatat ggactccgag gaggaaggat acttattagt
     6241 tagctgtgct ggtggtaaaa cttgtgttgt tagtttacct gttgaataca aggaagttaa
     6301 aggtgacaaa caaggattat tagttgaggt tactggacta ttaggtggac actctggtat
     6361 ggaaattgtt ttacaaagag caaatgctaa caaagcaata gctagagtat taagtgtatt
     6421 gaatgttgat tatgaattag cttctgttga tggtggtact aagcacaatg ctatacctcg
     6481 tgaagctaaa tgtgtaatag ctgttaataa agctgatgtt gaatctgcta aaaaacaaat
     6541 aaatgacatt ttaactgcat ttaaacatga gtttactact tctgaccctg gcatgactta
     6601 ctcagtagct gaaacttctg tagacaaagt tcttactaaa gattgtaaag aaaaagttgt
     6661 tcaaatgtct tgcttaactc ctcatggagt tcaatcagta agtcttgata tagaaggatt
     6721 agttgaaagt tctactaact tcgctataat tgaaactaaa gaatctacta ttgaattttt
     6781 aacttctgtt agaagttctg ttatgtctat tagagatgaa atcgctgata gaataagact
     6841 tttggctcaa gctttaggtg ctaattacga ccttatagct caatacccag cttgggaatt
     6901 taaaaaaggc tcaaaattag aaaaaatatg cagtgaaact tatgagaaat taactggtaa
     6961 agttcctact gttatggctc tacatgctgg tcttgagtgt ggattattac ttgataaact
     7021 tcctcatgct gaagctatct ctataggacc agatatgttt gatgttcata ctccaaatga
     7081 acatgttagt atcccttctg tagctaatgt ttgggattat gttattgaaa tattaaaatc
     7141 tatgaaccaa tattaaattt aagttttgat tctaagtaga ttttaatttt aagtaatcta
     7201 tataaaatta actctgtaaa ttaatactac tgttagtata atatttactt tcaatattaa
     7261 cttatagaaa taaagcctaa tgatagataa taacatgtat cattaggctt tattgtaatt
     7321 taaatattat taagctttat tctgttttaa aattaattct tatactattt taaatctatt
     7381 tcttccttgt ctcttagaat cataaagagc attatctgct atttgataca gttcatcata
     7441 ctcttgacca ggctttgcta taactacccc catactaata gataaacttt ctccatttgg
     7501 caacttaatg agatgaatat gattgtatac atcattacat ttattaataa gttcttcctt
     7561 atttattata tcattgatat aaacactaaa ttcatctcca cccaaacgac ctactatatc
     7621 attttttgca aaactttcat caagtacctt tgcaacatca ataagtactt tatctcctaa
     7681 caagtgacca taagtatcat taatcgactt aaaataatca atatctataa tcaataaagc
     7741 accatttttt tcagttgata aattcaacaa attttgtgca gttaattttc taatatatgc
     7801 tgcattataa atattggtta aactatccct ttgagcctta tctaacaatc tattcttttc
     7861 ctctttttca gaatcaatat tacatatttt cccgattaca tacattggta tttttctatc
     7921 attataaatt atttttgaag taactctaat ccaatgaaaa gtatcatcaa gcataataga
     7981 atacatttca gttgttccat tttcttgtga ttttattaca tcaaaaaact ttttattata
     8041 ttctttcctt attttatctc tttcattttc tatattgcca ttttcacttt ccttatcatt
     8101 tttatccaaa ccataataag tctcaatacc cttttctggc atagaaatta tcaatttatc
     8161 ttgcttataa tcatactcaa aaaagtattc acttgtcatt tcatacaact gagtatgttt
     8221 tttgagttcc aaagatatct tctcgttcat acttgtacga gtatgtaaac caaatgccat
     8281 aaatccaata atacaaatta ataaactggc tacaataaaa atagtctcaa ttggattctg
     8341 ttgcataata taaagcaacg agaagtcttg tctatatgta gtattttggt agacaattga
     8401 ttgcattttt tctgatgaaa tacttataat agctttatta aatatattca ataattcttg
     8461 tttatctttt ttaattatac caaaacaaat actctttgct ttataagttt gaggaactag
     8521 acgcacattt ttatacttag gttgattcat atagtactgc atagaataac cttcgccata
     8581 agtgtaatct gcatagcctt tattaacagc ttctatacac tcttctaaat tatcatacca
     8641 aactatattc tcttcaaact gaccataata attaatacct ttggctagag ctacacgttt
     8701 tccatctaaa ctattctcat ttaatttatt attaatcatc atagtatatt gagtagaaag
     8761 aaaaggccga gacataacta tattatgttc acgagagtat tcataatcat aaatcattcc
     8821 tgctacaata tcaatttcac gctttttagt catgttttct aaatcttctt gagattttac
     8881 aggcacaagt tcaaattcta agcctgtttt atcacatata tgatttaaca aatcaattga
     8941 tatacctctt aattcacctg tatctttatc tatattttga aatggtggtt tgtttaaaag
     9001 tactcctact tttaattttt ctgtctcttt aatgtaatca gtttcttcat cagataaaaa
     9061 catttgatta ttctctggtg taaagtattt ttcatataaa gtagttgaaa aatatgggtc
     9121 tttctgttca atattattaa ttgctgagtt aagcttttga gttaaatttg tactaccttt
     9181 aggagtaatg aaataaaatg gttttgatga aaattttgct acagtacgta caccttctag
     9241 tgggttcata ctcacattaa gaagtacatc tgctttacca ctctttagtg cttctagttg
     9301 ctcatcattt gttttacaat ataccaattc aggtgacata agattcattt tacaatactc
     9361 atctaactct ttaagtctag tatcagaatt ttctattaca gcaatacgga tttttttcac
     9421 atcttgtgaa ctcattatgt tatcaatatc tgtattttca tataatgctt gtagtactgt
     9481 atttgctact ccataactat ttcctgcata attatatatt ttttgcattg gctcattgta
     9541 taacatacct cccattatat caatttcacc attttctaac atcttcatta attttgtaat
     9601 agattcattt atctctccag gaacttgaac aaattcataa ttccatccag tatactgtgc
     9661 aatttctagt aaatattcat atgtataccc aaaatagtta ccttgctcat ctatttcagt
     9721 taaacctttt tgaataggat aagcaacacg aatggtacga ttatcattac ctatagcgtt
     9781 gataccaaaa gaaaatatgc aagaagtgaa tatagatgta attacaataa ttaataaaag
     9841 taattttttt aaattcacta cacaatcctc catatagtta agatttacag tataaattac
     9901 tgcataaact tgtagacatg taaaaataga ttagtatata aaaggatata taaatctact
     9961 tagaaattta aatatcctta atatatacaa tatatttaaa ttataactca tttatatgtc
    10021 tattacaaca aatttaccct gctaaatacc tactctatat cccataattt tacaagttgt
    10081 agtgaagttt aagttctatc tcacctcaaa tttagcagtg tttctattta cagttctttt
    10141 atatttcaca tctggataac ccaatataaa tgaagtgata acagcttcgt tctcgctcat
    10201 tcctaacagt tcttttatct ttggctctac atttgatgcc ataacaaaaa atccattata
    10261 acaaattcct aatccttgag aataggccat aagttccata ttagatgcag ccaatcctcc
    10321 atctacataa gcacttccac ctagagacat atctcctaca actaccataa gtagtggggc
    10381 atcataaaat aaagaatcat ttccgttctc tttgtatctt ttgtacattt tcttaaatgt
    10441 atttttatac atagaacgta cagggtcatt gtcatcagta tctaatgcca aattataaag
    10501 accttgtatt gccagttctt tcacttcttt tagcttttct tttactaata tgtatcttat
    10561 aggctgtcta ttaccacctg ttggtgtgta tctaccagct tccactatgt tttttatttt
    10621 ttcttcttct acctctatat ctttatattg tcttatactt cttctaaatt taataaaatt
    10681 caataacttt tcgggttcta attcaaaact ttcacgattg tattctatta cttcattttt
    10741 atcaaatcct tgcatttcta ttgcatctgt tgggcaaatt gccatacagt gaccacagtt
    10801 aatacagaga atatttttaa cctttgcttt atcatcctta acttttattg catttacaag
    10861 acagtctgat tcacacatac cacagcctac acatttttct aaatttatgt ctatcattcc
    10921 aaattattcc tccttttaaa aaatttactt tagtataaaa caaatagttg tctaagtcaa
    10981 tttttatttt tttaacaata tactagcctt aactacactt taatggagaa aaatatacat
    11041 aaattggaac ttaaattaga acttattcaa agtaaaagat aaaagaagta gatgtctaaa
    11101 agtgaacttt ttagttaatt agatattctt tattgtatga aatcaagtgg atatttatca
    11161 tttcaacaat aaaaaaggct tacctctatt ttggatatgt ccctgtcaag tagacagact
    11221 aaaaaaaggc tatctttatg cactgagata gccccttttc ccaactacta ttgtttatct
    11281 taatattagt caataaattt aatttgctat aacctgtaag ttacatttta atttgatgta
    11341 actttaaatt acatttttaa gtaatatctt taaattatat tcttaaaata tatcattaaa
    11401 ctacatttta attaatctaa aaactatatc ttaattaatc taagaaactt acattataat
    11461 cttttagttt cccattatca tatacaatct ttgcactaaa gatatttcca gtcttagccc
    11521 aatttaagaa cagcctgtca ccttcccaca tatttaaatc gaatatttta tcattatcaa
    11581 cccaaattaa atttccttct ttacaatcat ttaaatcttt ttcagataat tcaccttcaa
    11641 actcatctga gaaatacaaa aacatatacc aatcttcttc atctttaaat tttggaaaag
    11701 ttatcaaccc tttatatgac aatgatttaa gagtaagacc agtctcttct tttacttctc
    11761 taattacaca ttcttctgga gtctctcctt gctcaaactt tcctccaact cctacatact
    11821 taccttcatg tatatcatct ttctttttat ttctgtacaa catcaaagtc ttgtcatctt
    11881 tttctatata acaaatagtt gtaagtttca tgtatctcgc cttgctttct cttttatatt
    11941 ctcaactaat tattttcagc actttcaagc acatcactta tattcttcat tgcattaaga
    12001 gaaagctctg caaattcatc aaatggaatc cctatatctt ctatacttct cattgcatct
    12061 ctatttacat ttgaagcaaa tctcgtttct ttcattcttt taataacaga cttagtcttt
    12121 acactggtaa gttttttatc tggataaact aaagctatag cagttataaa cccagttata
    12181 gggtctgctg catatattgc tttttccatt aggctagttc ttgtaactcc cattccatca
    12241 tgatgaccaa gtattgctct atacatttct tcgtcaccga agttttcttt tttaagcagt
    12301 tctactgttg ttgtagcatg tccctcacat atttctcctg gctttcttcc aactatatct
    12361 gaatccaaat catgtaaaag acctgccact gcccactctt cttctttttc tggctctagt
    12421 cttttggcta attctctcat aacagcttca acagcataac aatgttttct cattctatca
    12481 gtttctaaat acttaaataa aatttctaaa gcttcttctc tcattttcac caatccccct
    12541 gttttttcac atatatgata tttttatata ttaatacaca taaattcaag tttttatata
    12601 tattcatgta tatttttcat gttatatcta ttctacacct ttatagtagt gtcctgcaac
    12661 tgttccaatt tcatctaatt tcttatacaa gtctgtagcc ttttttccaa gtttataatt
    12721 attactcaat acctcaatgt gagcttcaat aaccttttta accatctcac tatcttcaac
    12781 tgtaaaatct atcctaaata catctattcc aatttcctct aactcatata gattgtccaa
    12841 catacaagta accttcgaat tgtaaattgt acttctacag aatatatctt gtgttatcct
    12901 ataacttgca tccgtaaagt ctcttaaagc ataactactt tccttacatt tagcaactct
    12961 cttgtccttt ttacaatctc taacaactac tcccatagga cagtattctg taaccatcat
    13021 aggagcatat ccatatatta ctgcttcaat ttctacatct gtatatttta aagtttgttt
    13081 aatttcattt aagtttaatt cttgtgacaa acaaactgta cttgctccct cttctttgaa
    13141 gtgatttata ctttcactat tgaatgcatt taagtagcaa tctatattta agtctttttt
    13201 cttaaagtat tccatagagc cccagttacc aatttgaacc ttattaattc cttttatatt
    13261 atccaatatc tcaaattgct tgtactcttt atttctaaca atccttggtg ctgaatagat
    13321 taattttttg tcagtgactg ctgtattata gtggttctta tccaaagtat ctattttact
    13381 tgtatttgac aaagtatcta tgttatttgt atttgaaccc attagttctt taggaatacc
    13441 taaagatgaa gaagtatcta aatccataga aatacttgaa tctatagaaa tatttgaatc
    13501 tatagaaata cttgagacca tttgtaaagc ttttgataat gtatttatat cttcataata
    13561 gattaaatct acattataat ttaatactgc ttctaattgc tccaagttct taactttaac
    13621 tctaatctta ggagctgtat caagtttttt ctgtttattt actctatttg gctggtattt
    13681 taatctattc ttcttaaatt ttctatcttt tatctttatt ctttcttcat ctaaaagctc
    13741 aatacaatcc cttctcatct gatttaacac acttataggc atacttacac cttcatccag
    13801 gtcaatttct atctctttta acttatatgg tgtatttcca agcttttgaa tttgtgtttc
    13861 tattttttct ttacttaaag ccacctttat agctttttca actattttgt ctccttctac
    13921 caccgattta tttccacaaa aatcacttat aaaaagctct ggctttttac ctagtttaat
    13981 actcactttt gcacgtatat tagtttttat gaactcttta tcttgctgaa atgtagcttg
    14041 tactctatcc attaattcgc tgtcagaagt cttatatata agctgatttc tctttgcttc
    14101 tccaacaaaa tctaattcta tcaaatcccc tttatgtgct attgtagtta tctctccatc
    14161 ctttattatt cttccaatag tacctccacc taagttaatt ccatcacctt tcttaagcgt
    14221 attttctagc attactctaa gtcttttgtt ctttctatta taatctaaaa cttttccaat
    14281 atatagccct tgattattag gtctattaga gttcattata tccttaccaa cttcacctaa
    14341 tatatatcct tttgtaaact ttctattaaa tatggtatac aaattattta ttatttcctc
    14401 agaaatactt atttttttct tatctacaaa ttcattaatc gcttctctat atcctgaaac
    14461 cacagtagct acatactctg gctttttcat tctaccttca atttttaaag ataatacacc
    14521 tgcttcaatt attttatcca aatcttctat agtacttaaa tctctaggac tcaacaaata
    14581 atctccatta ctttttacta cttctcctgt atttatatct atcaactcat acttttgtct
    14641 acatggctgt gcacacctac ctctatttcc agacctattg cctatcatac tactcataag
    14701 gcattgaccc gagtaacata cacataaagc tccatgtaca aaaacttcta tatctacagt
    14761 tgtattttca caaatccatt ttatctcatc aacatttaat tctcttgcta aaacaactct
    14821 atcaaaccct acactctgaa ggtattttac atcttctaag gaatgtgcta ccatttgagt
    14881 gcttgcatgt agttcaaagt caggtaatag tttttttata agtcttgcca ttcctatatc
    14941 ctgtactatt aaagcatcaa tatctatatc gtataaaaat tttacatact ctacaaaatc
    15001 ttctatttca tactgtttta tcaatgtatt tacagtaaca aagacttgca ctccccttat
    15061 atgagagtat ttaacagcct caatcagctc atttctatca aagttatttg cagatgctct
    15121 agcactaaag tctttaccac ctaaatacac agcatttgct ccgttttgaa ctgctgcttt
    15181 taatgaatca aaagagccta ctggtgcaag taattctatt tttttcatat ttttctcctc
    15241 tatttatact tgttaggtta atataattat atctataact taaatatttg tattaaattt
    15301 atatatttct tactatattt taaattcttc aatcaattta tatattagtt atttatattt
    15361 ggttcatttg agttaacata tacattattt actatattta gtatatttct attaacttat
    15421 atattgatta ctgtatttta aattcttcaa ttaatttata tactgcttac taaatttaaa
    15481 acattttaat taatttgtac actgtttact atacttgaaa tacttctatt aatctatata
    15541 ctgcttacta aatttaaaac attttaatta atttatacac tgtttactat acttgaaata
    15601 cttctattaa tctatatatt gcctactata cttaagacat tttaattaat ttatacactg
    15661 cttactatac ttaaaatact tctattaact tatacattgc ttactatatt ttaaattctt
    15721 taattaatct atacaatatt tgctatatat aaagtatatc aaataacagc ttcatttttt
    15781 tgtaaattta cagaaagtta attttcatta aatgaatcat ctttattgat aaacataatt
    15841 ccaaagacca ctgccaaaat aaaaaaacat attagtattg atttttccac gaaaattttt
    15901 gtaaaaaaag ttcctaaaat agctccagat ataaagaata taataatagc ataatactgt
    15961 aaactctttt ttatcataaa aatattctta gtatgtatat aatgaaataa agtttctgta
    16021 gcacttctta aatttccagt acacattgtc gttgcaaaac tattcccatt tacttttcta
    16081 aaactctcta cttgcattga acaaataaaa gaaatagcaa catttacaaa catattaaat
    16141 cttcctctag gaataaatga aacaataagt attgtaatca tttcaattat aatgataacc
    16201 tgacgccaat gaatctttgt cttattattt gtaaaatact ttcttatctt ctcggctata
    16261 aatacaccta acataaatgc caaaatagga atcaaataat aaaatgattc ctttacatta
    16321 ccttctgcta tattaagccc aaacaaaact atatttccag tttgtgcatt agcaaagact
    16381 ttacctctta ccacataagt ataaatatcc aaaaatcctc ctgttattgc caacaaagaa
    16441 cataaaaaga atgattcaga catctgactc ttgcttgtca tatataaacc tctcgctttc
    16501 taatgataga tacaaaatca aaatcatttt agattctatt tttcattgat ataaaaaaat
    16561 tatttatatt taaatattgt ctatatttat acatcattta tatttataca ttatttgtat
    16621 ttatatagca ttcatattta tttatattta aacattatct atatctatat agcatttata
    16681 tttatttaca tttaaacatt atctatattt atatagcatt tatatttatt tacatttaaa
    16741 cattatctat atttatatag catttatatt tatttacatt taaacattat ctatatttat
    16801 atagcattta tatttataca ttaaaagaag ctgtctcaaa aaacaagaga cagcaaatct
    16861 tttagtttat tccacatttt ttgcatcatt atttaaccct gtacatttta actgtaaatc
    16921 ataagctttg ttttgaaact cagaagctaa gttttcagct atagtagctc tttcatttgc
    16981 ttcttcaagt tgagtactta actcctcaat cttgttttta taagcatcta actctgctct
    17041 agaaccttct gtcgtattac taagttcgaa tatttcttgc tttttatttt ctataatctc
    17101 aattaactct ttcatcttag tttcagcttc acgattttct gcttcttttt ctgctatcgt
    17161 aagctttaaa gacttgattt ctaattgaag ctcttcatta gatgctccaa ccttcttact
    17221 caattcttca ttagctttaa tcaacttctc attctcatct gaacattcaa ataaaaggtc
    17281 tgatatattt aatgcagtta atatcgcagc aactgataag ctcaatttcg ggttttgacg
    17341 tgttatttta tccatttctt tgtctacaaa atcagcaata ctaatcataa acttctctga
    17401 tttgtctcca accattggat attcagcgcc atggatttta accattactt tgttcatcct
    17461 aacctccccc gacattagtt gttaatttga tcttaagtta gcatttaatt tttcgcttaa
    17521 ttcacttaat atcttatcat gcaccttagc tacatcttca tctgttagag ttttatcttt
    17581 acttctataa gttattgaat atgcaattga cttgtatcca gcttctattt gagaaccttt
    17641 gtaaacatca aataacttgt agctttctac taatccttga ccattttcct ttattatatc
    17701 ttctatttgt tttacaaata catcatcttt aacaataaga gctatatctc ttgatgtaga
    17761 tggatatttt ggtaatggca cataattctt tgttctatca gaattatcaa atacaaagtc
    17821 aatatctatt tcagcgacat aaactctttg acctagattg tagttttcta taacatctgg
    17881 atgcaattca cctaatgtac ctacatattt attattatac actattttag cacatctacc
    17941 agggtggaag gtagtattat tatcttgagg ttcaatttca taacctttaa atccgacatt
    18001 tacaagtata gtttctatag ttccttttag tgcaaagaag tcaacatctt taccatacat
    18061 acctacacac attctatttt cttccttagg taattctgaa tcttgtggga tgaatatatg
    18121 tccacactca aatgcagata cttcttctat tttatgagat acattagttg ataaaacatc
    18181 taacatattt ggtattaaag tagttctcat tacagaagtt tcttctccta atgggttaag
    18241 taatttaaca aaatttctct ttgcattacc ttctggtact cttatcttat ctactccctt
    18301 agggcttaca aatgaatatg ttaatatttc atttaatcct acagcagttg aattatcttt
    18361 taaagcatcc atgaattttt gttttgaagt tttaattcct gctgtcgtat ttccttctaa
    18421 ttgtacagaa ggtatattct caaatccata aattctagct atttcttccc atacatcagc
    18481 ttcttgttcc atatctgttc taaagcttgg tacatctatt tctaatttat cattagctac
    18541 caaattacat ttaaactcta atgattctaa aattcctaca aactgctcca ttggtacatc
    18601 tacacctaat agatggttaa ttctttgagg atttactacc aattttttag gttctggttt
    18661 atttggatat acatctacaa caccttttaa aacttttcct gctcctaaca tttcaacaag
    18721 ctgtgcagct ctatttgctg ctatctctgc aagattaggg tctaagtctt tttcatttct
    18781 tgaagatgct tctgacctaa taccaacttt tttagctgtc attcttatgt tttctggttt
    18841 gaaattggca ctttcaaaaa gtacagtctt cgtattagaa gttatttctg agttagcacc
    18901 acccattaca ccagctaaac caagtgattt ttctccattt gttataacta gcatatcttt
    18961 gtctaatgtt ctctcaacat catctaatgt tacaagtttc tctccatctt tagcatttct
    19021 tactactatt cttccagtct ctacttgatt tatatcaaaa gcatgaagtg gttgaccaag
    19081 ctctaacatt acgaagtttg ttatatcgac tatgttactt ataggtctta ctcctgcttc
    19141 tgtaagtctt ctttgcatcc aatatggaga aggttctatt tttacatctg taaccattct
    19201 agcaacatat cttctacata aatctggatt atctatctca actttgaaat ctatctcttc
    19261 gtcactttct tttacttcga tttcaggata ttttactttt gttcctatag ttgcagctgc
    19321 ttctctagct atacccatca tgcatttaca atcaggtctg tttgaagtta attcaaaatc
    19381 tattaaagca tcttttaatc ctaaaacatc ttttatatct tttcctaatt cataagaatc
    19441 ttcgtggtct aaaatatata taccacctct tttatactcc tcaatgtaat gttcatctat
    19501 acctagttca gctgctgaac acatcatacc atctgataat tcacctctga aatcagtctg
    19561 tttgatttca actcctccag gtaacttaga accatttaca gctactggaa tataatctcc
    19621 ttctgatata tttgtagctc ctgtaactat ttgaacaact ttatctccaa tatctacttt
    19681 ggttacaacc aacttatcag catttggatg ttgttttatt tctaaaatct ttccaaccaa
    19741 tatattttct atttcttcac cataataatc tattgtttca actttagttc ctgtcattgt
    19801 cattttatca gcgaactctt ttacatccat gtctatatca acatagtctc taagccattt
    19861 taaagatact aacatctaca taccctccta attaaaattg atttaagaat ctcatatcat
    19921 tttcgaataa taatctaata tcatctattt catatttaag cattgcaagt ctttcaaccc
    19981 caactccaaa tgcaaatcca ctgtaaactt ctgggtctat tccacaattt ctaagcacat
    20041 ttggatgaac catacctgaa cctaatattt ctatccaacc ttcatattta cacattgggc
    20101 aacctttacc accacatttg aaacaagtaa catcaacctc tgcacttggt tctgtaaatg
    20161 ggaagttgtg aggtctaaac ttagttttga tatcagaacc aaacaatttt tcaacgaaga
    20221 tatccatagt tcctttaaat tctgccatag taacatcttt tccaacaaca agcccttcta
    20281 tctgatggaa cattggtgag tgtgtagcat ctggcgagtc tgacctaaaa catctacctg
    20341 gtgcaattac ttttattggt aactcttgac ttctcatagt tcttacttga actggagatg
    20401 tttgagttct aagtaatacc ccatcattga tatagaatgt atcgctcata tctcttgatg
    20461 gatggtcttt aggagcgttt aatgcgtcaa agttgttttc aacagtctca acttctggcc
    20521 cttctgctat agagaatccc attccgataa atatatctgt tacttcatct ataatttgag
    20581 ttattggatg cttcttccca acctttaaaa cttttcctgg ttgagtaaca tctatcactt
    20641 cctcttttaa ttttctttct ttttcaatag catttatttc ttctttttta gaatttatgc
    20701 taagttcaat gttttctctt acctcattgg caaccttacc aactactggt ctttcttcag
    20761 cagataattt acccatttct ttaagtatgg cagttatctc accttttttt cctaagtact
    20821 taactcttaa actttctaca ctttctatgc tttgtgcttc ttttatttca gccaaagctg
    20881 cttcacgtaa agcaagtaat ttttcttgca cacatatcac cctttcgttt ttttgtataa
    20941 aaaaagcccc tcaatccctg tctagggacg aaaggctata gtttcgcggt accaccctag
    21001 tagtttaaca atttaaaaat catcaaacca cctctaacaa acttaacgcg tttttgacgt
    21061 caaagcctac tgtaaaattt cagctctgtt actccagagg gaacttctca tgacctctta
    21121 gaaaacactc ccagcctagg tgtttatctc tttactaagt ttaatcaaaa tactttcctc
    21181 tttcattgca tttacaatat tatattgttt attttagtat tatatcattt ccattcaaat
    21241 tatgtgacta tttttaatat atatccaata cataattaat ttaattattt tttatataat
    21301 atacatctaa tgcataatta actcaattat tttttatata atatacatct aatgcataat
    21361 taactcaatt attttttata taatatacat ctaatgcata actaactcaa ttatttttta
    21421 tataatatac atctaatata taattaattt aattattttt ttaatatata ttgactattt
    21481 ttgatactct actatttaat tcaagattat aacatgacat tatatttatt acaatacatt
    21541 aaattaagta tttttttatt tcatacatca gtatagcaga gcttatcgca gcatttaacg
    21601 actcggcttt accatatata ggtatcttaa ccaaaatatc agactttgat acaagttctt
    21661 catttattcc atttgcttcg tttcctatca ccaatgctac ttttgagcca taatctattt
    21721 tgtcataaaa attttctgta tttaagtaac ttgaaactat attaaaatcc aatgatttaa
    21781 gcatgtccac agtttcatct tgtgaagcat caattatatt catatcaaaa atagaaccca
    21841 tagtagacct aattactttt gggttgtata tatcgacaca tccctttagt gctattatgg
    21901 catctactcc agcagaatca gcagtcctta ttatagtccc catatttcct gggtcttgta
    21961 ttctgtctaa aatcaataca aatttatctt ttttatttat actttcactt aatttttttt
    22021 tcttaaatga aactacacct attattcctt gagtattttc tgtatccact aattctttaa
    22081 agtttttatt agtagtcttg tatatctttg tgtttttttt atccaaatct tctaatagtt
    22141 ttacatgttc tttcttattt tcaaattctt cattgataaa tacataatca aggtttgcca
    22201 tacattcaag tgcaagcatt actattctgt atccctctat tatgaacttt gactctttat
    22261 tcctattctt tgattttaat agtgctcttg tatactttaa cttttcatta tccttactgt
    22321 ttatatttgt aatcatttta tctaaatgct aataacttat aaatttttat attagcacag
    22381 cctccttact tttaatttaa cttctcttta tctaatctta caaataaata ctaccttcga
    22441 ttaaaaaaat agtatcatat aactattcat aattatatga tactatctac tttcattgca
    22501 attaaatata tattaaatag taaattatct tcttctaatt atccccttat ttccagatgt
    22561 tatcgctgtt atactagtat tttgaccgat tataactagg atacttccgg ctgtaagttc
    22621 ctcatctgga gaaggtgtaa catttatatt tttacctgtt tttatagcaa gtacagttat
    22681 ctcatatcta gctcttaatt caagctctat aagtgtcttg ccaacccaac tatttggagt
    22741 tacgatttca acaattgaat actctgggtc aagttcaata tggtctaata tattatccga
    22801 aactaaattg tgtgcaactc ttactcccat atctctttct ggaaatacaa ctctatctgc
    22861 accaatttta taaagaactt tagcttgtaa ttcatccttt gccttacata ttatcaactc
    22921 tacacccatt tctttggcta taagagtcgc cattatagat gcccttatat cagaacctat
    22981 tgcaactact gctacatcaa agttacctaa acctaatgac cttaacgctt gctcatcagt
    23041 aacatccact ataagtgaat gggttacctt gtcagatata ctttgaactg aatcttcatt
    23101 tttgtctatt gccattactt gatgtcctaa aagatgcata gtagacgcaa ctgaacttcc
    23161 aaatctccca cacccaatga ctatatattg cttcatctcg aaacttcctc cctaatacac
    23221 aatttatata atatctctta aattaattgt ttacacatac aaaacttata cacaatatat
    23281 aattttatat acacagatta atttaatttt gatttacctt tttatttgta ttttatttcc
    23341 ctgttttttt atttcattaa ttaattttgt tcgttagttg attttatttc ttcatataat
    23401 tacatctagg ctcatttatt ttatcaacag atttacagtg ctactctaaa tccattataa
    23461 atttatacta ctccttggta acttaaccaa ccataatttt accttctgca tatctgattg
    23521 gttgactctt tcttctacca ccagaaagta tagccataaa taaagtaaga gaaccaactc
    23581 tacccataaa cataaaaatt attattataa tcttacctaa agcatttaag tttggagttc
    23641 cagctaaact tgaaccaact gtagcaagtg ctgatgtaac ctcaaaagct gattgaacta
    23701 atgtaaaatt aggctgagtt attgaaataa tcaatgttcc aaaaataact gctgatatac
    23761 taattacaaa tataccaaga gattttttta tagttgatgg agctaatctt ctttcaaaag
    23821 cttctatatc tgattttcct gatagaaaac ttctaactgt aatgattaaa acagctagtg
    23881 ttgttgtttt tataccccca ccagttgatg caggtgatgc tccaataaac atcaaaatta
    23941 tcattacaaa tacactactt tctcgtaaag tagctaaatc aagtgtatta aaaccagcag
    24001 ttcttgctgt tactgattga aatattgctg atagtacttt tccctttaaa ggcaagtccg
    24061 ccatagtagc tttttgatta aattctataa gaaaaataaa taatgcaccc acaaaaataa
    24121 gtgtagcagt tgaaaacaat acaaccttag aatgcacatt taattttgaa aatcttcttt
    24181 ttcttacaat atcaagtaca acagggaatc ctagccctcc aagcactata agtgcgcaaa
    24241 cagtcattga tactgtaaag ttgtttacat aagaatttaa cgatgtaaat gggccactta
    24301 cactgcccat caagtcaaat ccggcattac aaaacgctga tattgcgtga aatacactat
    24361 accatatccc tttagacaaa ccaaattgtg gaataaagac agttgataaa accagtgcac
    24421 ctattccctc tattgttatg gttattatta atacaaatct tgtaagtctt acaagtcccg
    24481 ataaatcagc ttgatttaat gactcttgta ttaataatct ttctcttagt tgtatttttt
    24541 ttcgtgccat aagtgaaaac attgtggcta cggtcataaa tccaagacca ccaatttgta
    24601 tcaatgttat tataactatc tgtccaaata aactccaata agttgctgta tctaccataa
    24661 ctagaccagt aacacaaacc gctgaagtag ccgtaaatag agcatttaat aagcctatac
    24721 tctctccact ctgcgacgct atcgggagtg ttaacaacaa tgctccgatt acaataatcg
    24781 cagcaaaccc agataccatt atttgagtag gacgcattcc atctgcgtag agaataagtc
    24841 ttctaactat tgttttcaac tttctttata cctccttaac ataaaatcac tactgttaag
    24901 acccttaatt tgtttatttt tagagctttc ctctaaatta ttattctatt atagtataaa
    24961 tattgtataa agacaatgat agtaattttt ataatttctg tttggaatat tttaaatttt
    25021 tatatatatc ttttaattta aattgcacca aaaacatcta tattttttac atataatagg
    25081 tctaaaacta tcatagcttt tactattttg tacttaaaat ttatttatca ttttaccagt
    25141 ataaatttca tatacttttt tgaagttaaa ttttttatat tatttataat cttatctata
    25201 tctttatgtt aaattttctt aacatgcatt ttttatattt aatatagtaa aataattaat
    25261 aactatatcc atgattggag gatttttatg gttaaatcca agaagaaaat atttgttttt
    25321 ctaattttaa ttgcagtttt tattatattt gcatcataca taagaaatta tactaatgat
    25381 acaaaaaaag attctgttag aggaactatt ccttttgaaa aaatggaata taagcgtcct
    25441 aatatacagt caatttgcga aaacattaac aaatgtaacg acaaactttt aacagctaaa
    25501 acagctaagg aacaattaaa tttatttaat gaagttgata aaatttatca ggatttttac
    25561 agtacactta ctatagcaaa aattagaaat aacatagatt catcagacga attttactct
    25621 aaggaatata agtatcttat gagtaaatca gtagatgttg acatgtcata caaagacttt
    25681 caagatagat ttgtaacttc taaattctct aaagaattag aaaaagaact tggaaatgat
    25741 acatttaatt atctaaagaa cattggaaaa cttaattcta aagaagttga aggtttgctt
    25801 aaaaaagagc aagacctagt tgtaaaatat gaagacttat tagctaaatc tacagtatca
    25861 atagatggta tagaggtaga ctttgaagaa gccttgtcaa gaccaaatct tagccctgaa
    25921 gaatatgtga aaatttattc agattactta aaaaaataca atcctatatt tggtaacata
    25981 ttcttagaac ttatacagac aagaactgaa atagcaagta agcaaggatt taagaattat
    26041 atagattatg cctatatgaa cctaaacaaa gactattctc aagaagaagc taaaaaattt
    26101 agacaagatg taaaagatta catagttcca ttatatagag agatttcatc taaaccatct
    26161 gattcttcta tttatattaa agtatataaa aatcgctcat ttaggaagtt tgataaagta
    26221 cttgaagata tatctccaaa actaaaagag agcttcgact atatgaaaaa atatgacttg
    26281 tacgattatt cttctggaaa aaacaaatca cctggaggat atactactta tataaataaa
    26341 tataaagcac ctttcttgtt taatacatgg gacaattctt ttttaggtgt tactagtttt
    26401 gctcatgagt ttggtcattt ttataattac tacaacagta ttaacttaaa taataagatg
    26461 caaccaagta tagatgtatg tgaagttcat tcaacatctt tagaaattct attttataaa
    26521 tattttgatg atttttttgg taaacaatct gaagctataa aaaaagaaca cttatcgatt
    26581 gttttaaata ctattattga tgcttgtctt tatgatgaat ttcaagaaat tatatacaag
    26641 aatccatata tgagtctaaa tgatatcaat aaattgtttt ttgatttaga ggaagaatat
    26701 ggagtttcca ataatatact tagagaaaaa aatgctcctt tttggatact tgtatctcat
    26761 aacttccaag ttccttttta ttacttgagt tatggtcttg catctgatgt atcacttcag
    26821 atatggcaat tatcacaaga agattacaga aaagctgttg atgtatatat ggatttttta
    26881 aatcaaaata ctgatgctgg atttaaagat gtggtagaaa aggtaaattt acaattacct
    26941 ttccaaaatg gaaatttaga ggaaataagc tctactttat atgattattt tggtatagat
    27001 aatccacttg aactaaaaaa tgcttcttaa tttctaatac tttatactca ctcttaagat
    27061 ttagtattag attgaaataa gtgatatttt ttcacacaca attaatgttt aataaaatta
    27121 tatggtaaag tatcagaaat ttttaagact cttgtcttta taatttctga tactttattt
    27181 attaataata ttattatttt tttatatata taattaaaag gattgtggaa atcaataaaa
    27241 atatccctgt aaaaatagca ctattcatat ggaacttaat agaacttgca tctgttacct
    27301 tattattctt aatccagaac ccacaaacca tagttgaaag aaaaagtaaa acagtgataa
    27361 gacttgaacc aatagttata attttcatct tacatcaaca tcctctccaa taatattttc
    27421 tattaaaata tcaatgaatt tatctcgctc atatttattc tttatattta taccactgta
    27481 taactgaaat gattctgtag ataaagcaga aatctgtatt ggtaaaataa tttgcagggc
    27541 aatgattctt agactcattt catctttttt atttccaaaa attttacgaa gcatagggac
    27601 aatatcaagt tctgcttgca tatctccatt ggttatacat tgattcaata caaaaggaag
    27661 cactttttca taatgtaatc cagtatcaca caattttttt aataaatttt ttaaatcttc
    27721 aataggtctt agagtatata cactatcgtc atatagctct gctattatat ttgacatcac
    27781 atctcctatg gcagtactca acaaattatc ctttgtttta aaatgataat ttattaaacc
    27841 aactccaaca tttgcacgct cagcaatttt tctaactgta atctcctcaa tatcatctac
    27901 ttcatttaaa agattcaatg taacttcaat tatacgttct ctagcatctt tatcattact
    27961 tttcataata gacctccaaa acaattaaca ttttcagttc tgaacatgtt cagtataata
    28021 tttttttcat catatgtcaa tataatttaa ttataatttt agttgactct aacattatga
    28081 tatggtttat attaatatta agctaaaagt acctttataa aggagacaaa aacatgttta
    28141 agataggcga tttttcaaag ctaagtaaaa ttagtattcg tatgttacgt cattatgatg
    28201 aaataggact tttaactcct tctcatacga ataaaactaa tggatataga tactatagtg
    28261 cagaccaact ttctactaca aacagaatcc atgcactaaa agatatgggc tttggcttgt
    28321 attctataaa agaaattttg acagaatata acgataaaga aagtctcata aaatatctaa
    28381 atatccacca ttctcaagta aaagaacaat tagaagatac acagaaaaaa ctattaaaaa
    28441 tagaaaccac tattaaaaga attggaggaa atgatattat gaaaaattat gatgtaacta
    28501 taaagaattt tgcaccaaaa tatatgatga cacttagaag ggttatacca acttatcaag
    28561 acgaaggaat gttatggcat caagcatttt tagagacaaa agaccagaat gttcaaattg
    28621 aacccccaaa gtattctaaa gcagtttttt atgatactgg atataaagaa gattatgtag
    28681 atgttgaagt acaagttgct gtatctggaa agtataaaga tactgaacat gttaaattta
    28741 agactgttcc aagtgtgact gctgcaactg caattgttaa tggaaatttc aatcaagttg
    28801 cagacgcttg tgaggctatt ggtaattgga tatcagataa taactatgat gtggatggtc
    28861 ctatgtttaa tatttatcat gtaagtcctg gcaacgatag taatcctgat aattgggtaa
    28921 cagaggtatg cttcccagtt aaaaagaagt aatttttata ttaattggat aatattttaa
    28981 tttgttagac aaattacttt tagtgttatg tataaatacc tacaatttga ttaggatatt
    29041 atcttaattt gttaaacaaa ttacttttag tgttatgtat aaatatctac aatttaatta
    29101 ggatattatc ttaatttgtc agataaatca ct
//
LOCUS       DAGSHT010000029        28932 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGSHT010000029 DAGSHT010000000
VERSION     DAGSHT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.030"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            368..1489
                     /locus_tag="MJB72_001184"
     CDS             368..1489
                     /locus_tag="MJB72_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY8262309.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            1525..1938
                     /locus_tag="MJB72_001185"
     CDS             1525..1938
                     /locus_tag="MJB72_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8262310.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            1931..2470
                     /locus_tag="MJB72_001186"
     CDS             1931..2470
                     /locus_tag="MJB72_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8262311.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(2670..3155)
                     /locus_tag="MJB72_001187"
     CDS             complement(2670..3155)
                     /locus_tag="MJB72_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262312.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            4060..4437
                     /locus_tag="MJB72_001188"
     CDS             4060..4437
                     /locus_tag="MJB72_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY8262313.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            4833..6638
                     /locus_tag="MJB72_001189"
     CDS             4833..6638
                     /locus_tag="MJB72_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8262314.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            6715..7254
                     /locus_tag="MJB72_001190"
     CDS             6715..7254
                     /locus_tag="MJB72_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBY8262315.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            7868..9082
                     /gene="dpaL"
                     /locus_tag="MJB72_001191"
     CDS             7868..9082
                     /gene="dpaL"
                     /locus_tag="MJB72_001191"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBY8262316.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKLLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            9412..10599
                     /locus_tag="MJB72_001192"
     CDS             9412..10599
                     /locus_tag="MJB72_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBY8262317.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            10615..12198
                     /locus_tag="MJB72_001193"
     CDS             10615..12198
                     /locus_tag="MJB72_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBY8262318.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIRFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKNRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            12226..13554
                     /gene="ssnA"
                     /locus_tag="MJB72_001194"
     CDS             12226..13554
                     /gene="ssnA"
                     /locus_tag="MJB72_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBY8262319.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISVEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            13699..15051
                     /locus_tag="MJB72_001195"
     CDS             13699..15051
                     /locus_tag="MJB72_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBY8262320.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            15243..16352
                     /locus_tag="MJB72_001196"
     CDS             15243..16352
                     /locus_tag="MJB72_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8262321.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAVKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            16635..18026
                     /gene="hydA"
                     /locus_tag="MJB72_001197"
     CDS             16635..18026
                     /gene="hydA"
                     /locus_tag="MJB72_001197"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBY8262322.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKRCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            18020..20581
                     /gene="xdh"
                     /locus_tag="MJB72_001198"
     CDS             18020..20581
                     /gene="xdh"
                     /locus_tag="MJB72_001198"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBY8262323.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            20733..22079
                     /gene="rpoN"
                     /locus_tag="MJB72_001199"
     CDS             20733..22079
                     /gene="rpoN"
                     /locus_tag="MJB72_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBY8262324.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            22861..23883
                     /locus_tag="MJB72_001200"
     CDS             22861..23883
                     /locus_tag="MJB72_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY8262325.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            23943..24950
                     /gene="gap"
                     /locus_tag="MJB72_001201"
     CDS             23943..24950
                     /gene="gap"
                     /locus_tag="MJB72_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBY8262326.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            25172..26374
                     /locus_tag="MJB72_001202"
     CDS             25172..26374
                     /locus_tag="MJB72_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBY8262327.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            26400..27143
                     /locus_tag="MJB72_001203"
     CDS             26400..27143
                     /locus_tag="MJB72_001203"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBY8262328.1"
                     /translation="MRKTIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            27152..28684
                     /locus_tag="MJB72_001204"
     CDS             27152..28684
                     /locus_tag="MJB72_001204"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBY8262329.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPAGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT        11579 a         3260 c         5614 g         8479 t
ORIGIN      
        1 tctttaccac tgttttatat attttaaaat agacacaaga cctatttcta aatcttttag
       61 gtgaaatttc tttttaaaca tatctaaaaa gtatttgtag taatattaat ggactgaatt
      121 tcttaaagta tatatttctt tacatgttgg agtttgtgaa gtttgagaat tggagggtga
      181 aagaaacatg taatggatta atattaaaga actgtatttt tataatattt gtcttaaaaa
      241 taaaaaaatc tattgacttg aagttaactc caagtagtaa agtataatta tacaagaatt
      301 atatacttgt aagaatagtt aaagtagaga gaaaaaacaa acaagtttta aagggggaag
      361 taaaacaatg gaggcaaaaa aattaggatt tggattaatg cgtttaccaa taaaagatga
      421 aaatgatgtt acaactattg atatggaaca tttaaataaa atggtagaca cttttttaga
      481 aaggggattt acatattttg atacagccta tgtgtatcac atgggaaaaa gtgagatagc
      541 acttagagaa tctctagtaa aaagacacaa aagagagagt tttactatag ctacaaaact
      601 tccattaatg gctttaaaga aaaaagaaga gcaagaaacc atttttaatg agcaattaga
      661 aaaatgtggt gtagactatt ttgactacta tcttttacac aatataggcg tgtcacatta
      721 tgaagtagct aaaaaatttg atagctttaa gtttattgaa gaaaagaaaa aagaaggaaa
      781 aataaagaat attggttttt cttttcatga tagtgcagaa ttattggata aagtcttgag
      841 tgagcatcca gaagtagact tcgtgcaatt acagattaat tatttagatt gggataatga
      901 aagcatacaa tctagaaaat gctacgaggt ggcagagaag cataataagc ctgtaattgt
      961 tatggagcca gtaaaaggag gtacacttgc aaagatacca gaaaaagcag agaaattatt
     1021 gaatgactac aatccagata tgtcaatttc atcttgggca atacgttttg cagcaagtaa
     1081 agataatgtt atgatggtct taagtgggat gtcaaatatg gaacaattat tagacaatac
     1141 aggatatatg caaaatttca aaccattcgt acaagaggaa tataatatta tagatgaagc
     1201 agtagagatt atcaacgaat caattttagt tccttgtact gcctgtcagt attgtgtaga
     1261 aggctgtcca aaaaatatcg ctattccaaa ctattttgcg ttgtacaatg cagaaaaaca
     1321 agctcttaat actggatttt caacacagat ggtctactat aataattata ctaaaactta
     1381 tggaaaggcc tcagattgta ttgaatgtaa acaatgtgaa gaaagctgtc ctcaacacat
     1441 taaaattata gatgctttaa agaatgtagc agaaaccttt gagaaataat tcaatctata
     1501 caatatcaag aggagaagat atgtatgacg atttcagaag ttagtaaaaa atacgagctt
     1561 tcagcggata ctttacgata ttatgaacga attggtttaa taccacctgt aaatagaaat
     1621 aaaagtggaa ttaggtcttt tacagaagaa gattgtgagt gggttaattt cataaagtgt
     1681 atgagaggtg caggtctttc tattgagaca ttaattgagt atgtagctat gtttcaacaa
     1741 gggagcagta ctatcaaagc tagaaaagaa cttttaatag agcagagaaa ccaactagca
     1801 aaaagaatag aagaaatgca aaaaactttg gaacgattaa attttaagat tgatagatat
     1861 gaagaaggta ttattgagaa agaaaaagta ttaaagagca gtagaaataa gaaaatggag
     1921 gcagtatctt atgagtaaaa atgtattaat agtatcagct agtcctagaa aaggtggaaa
     1981 ttctgattta ttgtgtgacc agtttataca aggggtatta gaatctaaaa atcaagtgga
     2041 gaagatattt ttaaaagata aagatattaa ctattgtact ggatgtggtg cttgttatga
     2101 aaaagaagca aattgttcac agacagatga catgggagaa attcttgaaa aaatgattga
     2161 ggcagatgtg attgttttag cgactccagt ttatttttat actatgaatg cgcaaatgaa
     2221 aactatgatt gatagaactt gctctagata tactgaaatt tgtgataaag atttctattt
     2281 tatcgttaca gcagcagatg aaaatgtggc tgatatggaa agaacaatag aaggatttag
     2341 aggatttaca tcatgtctaa atggttctaa ggaaaaaggt atagtatatg gtgtaggtgc
     2401 ttggaatgta ggtgatatta agggaagtaa ggcaatgaaa caggcatatg aaatgggtaa
     2461 gttggcgtag tatgtttgta taaaattgta taaaattgaa tatattctta tcatttcaac
     2521 aataaaaaga ggcttatctg tattttgaga cagcctcttt gtgctatatc atttattttt
     2581 attctatatc cttattatac cctttatttt tttatgccta tttaagtatc tgttgttact
     2641 catatatttt aataattacc cttaaaaaac tatacagatt cctgcataat attattttct
     2701 ttagtaatta atttttctag gtttttccaa tccatttctt ccatatttgc aaaataatga
     2761 ccacttccta tgcaagtcag gtaagacttt ccatctaatt caaacgaaga acctccaata
     2821 cactttgaat tacatttctt acaagtacga gttttagtta tactatcttt aatagtttta
     2881 gagcattcct ccacagcatc tttatatgta tttagatgaa aaagatttgc tttaactctc
     2941 catttttttt cattacattc aatagtaaat aagctacgtt ttggtttctt agttgaatat
     3001 actattttgt aagaacgagc acctgttttt ggttcaattt tacaatctaa tgatttacca
     3061 agttcatgta aatgacatgc aatatcataa tctttaccac ttagtacttc taacatttct
     3121 tctgttgttt ctgctgttaa ttcacttttg ctcatattaa aaaaatcccc ctatataaat
     3181 aataatttca tatgaaaata attaatataa gtatattaaa taaaactatt aaaatcaata
     3241 aaattactgc aatcttacat tattgggcat agaatgagag ctaatgattt attagaaata
     3301 agacttaata gtatgaaaat ttacttagac gtggtacaat tctataaaag atttaaaact
     3361 aggaaaggag aaagttgttg agatacggcc aatataacaa tttatttata agttgtaaat
     3421 ttattatgag attaattaga ttgttaagta atactatcaa aaggtagttt tttggatttt
     3481 cagcaacagt ggtagatatt gataattttt tggttattat tatatttagc tgttatattt
     3541 tcaataggct ctatgataac tagggtaaat aaggtatggg taaaggtttt attatttata
     3601 atagtgggat tgtcaactat agttctttca tatgtaataa cttgattatg caaaattcta
     3661 tgaagcagga accctgtgac tttagtcatg ggaggttcag aatttaaggt taaaaaataa
     3721 ctttaatctg taaattctca aaacaagaat tctcatgttg gtaaatttct agatatgaga
     3781 aaaaaattga catcataaaa aagtaatttt agagcgtttt tttacgaaaa aattcagaag
     3841 taactatttt gaatgacttc acaaaatttt atatataaat aaagtttggc acgttaattg
     3901 ctttaataaa agttaacaag acacaaataa acataatcat ctaagtattg attatttgaa
     3961 gttttagatt tgaagtttaa tctaaaggtt cgtattaata ttggtgttta ggaaatttta
     4021 ttttcctgta aatataactt aataatagga ggtgaagtca tgtcaaaagt agtaatagct
     4081 acagagaaag ctccaggagc aattggacca tattctcaag caattcaagt tggaaacata
     4141 gtttctacat caggtcaact tccaatagat atgaagactg gcgagttagt atctgaccca
     4201 aaagaagcaa caaaagcttc tttagataat gtaaaagcaa tattagaaca agctggtaca
     4261 agtatggata aaatatttaa gactacagta ttcgtaaaag atttaaagga ttttaatgat
     4321 attaatgaag tatatgctac atattttaaa gaaaatccac cagcaaggtc atgtgtacaa
     4381 gttgctcaat taccaaaaga tgcagtttta gaaattgaag caatggcagt attataagta
     4441 ttataacaat aaaaccaata acaaaactaa taaaaattaa aaactaataa aaaaagtcta
     4501 caattaaaaa agtttatatt aaaaaagtct atataaaaat ctatctaaat atttaactat
     4561 tgagcttttc tcatataatg tatattttta tgtcaaaaat atatttgtta tatgaggaaa
     4621 gctttttttt attattgtat tgtgatttat tttattgtct gcatattata attaaggtta
     4681 ctatataaaa caaacttttt ataaattaat attgtctgtt ttaacgcaaa tatataaata
     4741 agtaatgttt tatatatatt ttaaagagtg actatatgtt aaaatatagc taaactgata
     4801 atttggtaaa ttaaattcta atgggggaaa ctatgttaag taaattaaaa gaatttcaac
     4861 aagaaatgat aaaatacact gaaacagtag ctagtgtgtt agatgtagat attgaaatag
     4921 ttgacgatag attaataaga attagtggta caggtctgta taaatcaaag ataaatgaaa
     4981 gcgttgttac tgagggcttt atttatgaca atgttataca gacaggtcag gaattagtcg
     5041 tattggatat atgtgataat cagctttgta tagaatgttc ccattacatg aaatgcctaa
     5101 ataaagtaat aatagcagtt ccaataaagt ataataatcg aactatagga gtaattggtg
     5161 ctattagtac agataaaact aaaaaagtag aaataagtgc aaaaatagat aattatttaa
     5221 aatttgtcaa tcatatatgc gatttaattt ctatgaaaat agaagaacat gaagcgtcaa
     5281 aaaatagcag tagaaaaatg gatatgatga tagaaatcat tgaaaatgtt gaaaaaggcg
     5341 ttataatact agacataaat agcaaaatat cttatataaa caatatagct ctaaaaaaat
     5401 tagatataga taaaaatata atagaaaata tagtaaatat agtatctgtt gaatcttcct
     5461 caaatggaca tgaattacta gaaattgata ttgataacaa aatttataat ataaatgcca
     5521 aaataattcc ggtatatcca tatataaatc aatacgacaa aataataata tttgacaaga
     5581 catatatcaa tcataaagga catggaaaag tgaatagtgg ctggggaaat agtgatatag
     5641 agtctataat tggaaattca gaagcaatgt taaaagtaaa agagagaact aagaaattag
     5701 caaagtcaaa ttcaactgta cttataactg gggaaagtgg tacgggtaaa gagctaattg
     5761 ctagggctat acatgctgaa ggtagcagat ggaacaagcc atttattgcg ataaactgtg
     5821 ctgctattcc agaaaatctc cttgaaagtg agctatttgg ctatataaaa ggtgcattta
     5881 gtggagctag tagtggaggt aaagtaggta aatttgaact tgcaaatgaa ggtgttatat
     5941 ttcttgatga aataggagat ttgtcaatgc ctttacaagc aaagttactt agagttttac
     6001 aagagagaaa atttgcgaga attgggtcaa ataaacttat agatttagat ataagagtga
     6061 ttgctgctac aaataaaaac ttattgaaat tagtaaatga aggtaagttt agggatgatt
     6121 tatactatag attgaatgta attcctataa atttgccacc tcttagggaa agaaaggatg
     6181 atattgaggc aataatgatg aagtttgctt ctaaatattc tttagaatta ggtattcagt
     6241 taaataaaat agaagaaaat gtaatgaata tgttgattaa ctataattgg ccgggaaata
     6301 taagggaact ggaaaatgca gttgaatata tgatgaattt ggttggagat gatggaataa
     6361 tatataaaga tatgcttcca ttagatattt taaactatta taatattaat ggaaatattt
     6421 ataaaaataa ggatgtaaat ataatatttg aagatgatat agttggaggg atagtagaga
     6481 atcaagaaag gatactttca attaaagaat tagagttgac ttacataaac aagttgttga
     6541 ataagtatgg aagagataca aagacgaaga agaagatagc aaaagactta ggcataggtt
     6601 tggctacact atatagaaaa ttggaagagg aacagtagta atataatatt aaaatgatag
     6661 gtatttgtgt gtttttatag ataggtattt ttactttatg gtggaggtgt tattatgcaa
     6721 tatgcgatag agctatatta tgataaaaaa acggaaaagc aactttttga tttatctaaa
     6781 aaaatcgctg atgaaaaaat tagtacgaag tacttagaat ggaaaacaag acctcatctt
     6841 actttggctt gtttcaatga tgtggatgag gcttgttgca tagacaagct aaaggggttt
     6901 gcacaaaacc ataaggtaat acctgcttac attggttcag taggcatgtt taatgatact
     6961 aaaacggtgt ttgtatcacc tataatgaat agcagtatgt atcagtttca gagagaatta
     7021 tatgaggtac tacaagattt tgattcaaat ggttgggaat ggtaccaccc caatcgttgg
     7081 gtaccccatt gcacaatagc acttactgga gatgatgaaa gtgaggcttt ttttcgggca
     7141 agtgatgtat tactgcgtga gttcaaaaaa ataagtggtg agtttgtctc aataggattg
     7201 gttaaaatta catttcctgt ggaagaaatt tttacagtga atatgaatag gtaactttaa
     7261 ctctgttgtg gatgttatga atattttgta tagtgcattt catcagataa atgcgaagtt
     7321 gcgaatttcg acagctcaat agtcgcaagt agggcacaat ggtacacata ttgcactttt
     7381 gcttattggg gggacgccca tgtaaacttc tgtaagaagc tatgaaaaga tttaagccat
     7441 tttggaggct ttgggtaata aagaatataa agtatataaa gtatataaat ttatagagtt
     7501 tatgcgtaag aaggttatag ccatatattt atagaaaata aaagtagtaa tatatctgac
     7561 aaaacaatag acatataaaa aataagagca taataaagga agaattaaga caaataaacg
     7621 atttctcgaa ttgataattc tcgtattgat aaaaatttcc caatatgaga attttttctt
     7681 ttataaatct tatttttcac actcaaaatt tataaacttt aaagaaattt tataaaagta
     7741 tgtaattcca acataaaaaa attgttttta gaataaataa tattggcacg gtaattgctt
     7801 tgctataagg cacaaagata aaaaattaaa atgacaccga aaaatttgaa aggggaatat
     7861 aaaataaatg agagaaataa aatggaaatt aaataactta cctaaatcag aggaaaaaga
     7921 aaaaggaatt gaatttttaa aagatgaaga gattgcaaag gctaaagctt ttcatgagag
     7981 ctttccacaa tatgaaaaga ctcctttagt taaattagat aacttagcaa aattactagg
     8041 cgtaagcgga atatatgtta aagatgaatc ttatagattt ggcttaaacg catttaaagt
     8101 tttaggtggt tcttactcta tgggtagata cttagctcaa agattaaata cagatatatc
     8161 tgagttaggg tatgataaat taacttcaaa agaaataaaa gaaaaattag gagaaataac
     8221 tttctttaca gctacagatg gaaatcatgg tagaggagtt gcttggacag ctaataaatt
     8281 aggtcaaaaa tctgttgtat taatgccaaa aggttcttct gaatttagat taaataaaat
     8341 caaaggagaa ggtgcagatg ctagtataac agacttaaac tatgatgatg cagtaagatt
     8401 agctaatgat tatgcagaag ctgatgacca tggagtaatg gttcaagata cagcatggga
     8461 cggatatgaa gaaataccag catggataat gcaaggatat ggaactatgg ctaaagaagc
     8521 tatagaacaa ttaaaagaat atggagtaga tagaccaaca cacgtatttg ttcaagcagg
     8581 agttggttca cttgctggtg cagttcaagg atatgttgct tcaatatatg atgaatgccc
     8641 aataacagta gtagtagagg ctgatgaggc tgactgttac tataaatcag cagaagctgg
     8701 agatggaaaa ccaagatttg ttggaggaga tatgccaact ataatggcag ggcttgcttg
     8761 tggagaacca aacactatag gttttgaagt gttaaaaaat tattcatcag cattcgtttc
     8821 agcaccagac tgggtatctg ctaaaggtat gagaatatta ggaaaccctc taagaggaga
     8881 tgagaaagta atctctggag aatctggagc agttacaact ggagcattag tttcaatcct
     8941 tgaaagtgaa gatttaaaag acttaagaga agctttgaaa ctagatgaga actctaaagt
     9001 attattaata agtacagaag gagatactga ccctgataaa tatagagata tagtttggaa
     9061 tggtgaatgc caaagtaaat aaaagactta aggaaaagaa aatcttaaca aataaatgaa
     9121 aaatttaagg aaaataaaaa cttaaataag tgaaaacttt aaataaaata aaaactaaaa
     9181 caaatgaaaa atctaaataa atgaaaaagt aaagcaaata aaaaagtaaa gcaaatgaaa
     9241 aattcaaaat aaaaaccaaa taaaattaaa ataaagtcta aataaaaatt taataaataa
     9301 aatataaaga aataaaaaat caatagatta agtgaaatgc taataaatta aaaaaatgaa
     9361 atatcaaaat aaaacgtcaa caaattaaaa attatatggg ggaatatcaa aatgttaaat
     9421 gaaacaagaa aaaaacaatt aactgaagta tgccaagact taataagaaa tgctagttat
     9481 tcaggtcaag aagaaaacgt agtaaaagct atagaagaaa attttgcttt actaggtttt
     9541 gatagttggt caagagatag atatggaaat atagtaggat gtataaaagg aaataaacca
     9601 ggtaaaaaaa tattatttga cgggcatata gatacagttc cagttccaga tgcaagtaaa
     9661 tggagtgttc ctccttttga agcgagaata gtagatggga aaatatatgg tagaggaact
     9721 tctgatatga aggggcaagt aagtgctatg atggctgctg tttcatactt tgcagaagat
     9781 actaacaaag actttgaagg tgaattgtat gtagcaggtg ttgttcatga agaaatattt
     9841 gaaggtgttt cagctagaga gataagtaag gctgttcaac ctgattatgt tgtaataggt
     9901 gaatcttcag aattaaactt aaagataggt caaagaggta gaggagaaat agtagtagaa
     9961 acttttggta aaccagctca ctctgctaat ccagaaaaag gtataaatgc agtttataaa
    10021 atggctaatg taatccaaaa gatacaacaa ttagtacctc caactcatcc agttttaggt
    10081 gatggaatat tagttttaac agatataaaa tcttctccat atccaggagc atcagtagtg
    10141 ccagattatt gcaaagctac ttttgataga agattattag ttggagaaac aagagaaggc
    10201 gtattagctc caatacaagc tttattagat gaaatgatga aagaagaccc agaattaaat
    10261 gcaaaagttt cttatgcagt tgaaaaagct gattgctata caggaaatac tatagaaagt
    10321 gaaagattct tcccaggttg gttatatgac gaagaagatg aatttgttca agcagcgtat
    10381 aagggattaa aagaagctgg aatagattca gaaataactc aatactcatt ctgtacgaat
    10441 ggaagtcact acgcaggtga ggctggaata agaactatag gatttggtcc ttctaaggaa
    10501 aatcttgctc atacaataga tgagtatata gaattagacc aattattcaa aggtgcagaa
    10561 ggatattatg gtatattaaa atcagtttat aaaaaataat taggagatgg ttttatgaga
    10621 attttaataa aacatgggct tatagtagat ggaaataaaa caccagcata tgaaggagat
    10681 attttaatag aaaacgagaa aatattaaaa atatcacaag atttaaacga agaagctgac
    10741 aaagttatag atgcaaaagg tagagttata tgtccaggtt tcatagatac tcatagccat
    10801 tcagacctag ttattttagt taatccatat aatgaggtaa agattagaca gggaattaca
    10861 acagaagtgt tgggccaaga tggtatatct atggcccctc tacctcaaga gcatatatct
    10921 tcttggagaa aaaatttagc aggtctagat ggtgaaagtg atgaaattga ttggaaatat
    10981 gaaacaacag agaattattt aaaaatgatg gattacaatg gggtaggttt aaatgaaact
    11041 tatttagtac ctcatggaaa cgttagaatg gaagctatgg gacttgaaga tagacctgct
    11101 actaaagaag aaattcaaaa aatgtgtgaa atcacagaga gagaattaaa agcaggagct
    11161 ataggattgt ctactggact tatatatata ccttgtgcat actcattgac tgaagaaatt
    11221 atagagatgt gtaaggtagt agcaaaatat gacggagtat ttgtagttca ccaaagaagt
    11281 gaagcagata caattttaac ttcaatggaa gaaatcatag aaattggaaa acaatcagga
    11341 gtaaaagttc atttttcaca ttttaaggtt tgtggtaaag caaactggaa gtatatacca
    11401 caagttatag agcttttaga aaaagcagaa aaggaaggaa taagagtatc atttgaccaa
    11461 tatccatatg cagcaggaag tacaatgctt ggagtagttt tacctccttg ggcacattct
    11521 ggaggaactg ataagttaat agaaagatta agtgatgaaa acgaaagggc aaaaatgaaa
    11581 aaagatatag caaatggaat agaaggctgg gataatttta tagaatttgc tggaatagac
    11641 caaatatttg taacaagcgt aaaaacagaa aagaataaag atacaattgg taagagctta
    11701 ttagaaattg gaaaaatgag aggaaaagac ccattagatg caacgtttga tttattaaaa
    11761 gaagaagaaa atgcagttgg gatggtagat ttctatggat tagaagaaca tataataaga
    11821 ttcatgaaaa gagacgagca aaatgtgtgt acagatggtc ttttagcagg taaaccacat
    11881 ccaagagctt atggttcttt ccctaaaata ctaggaagat atgttagaga gttgaatgtg
    11941 ttaacaatcg aagaagctgt ttataaaatg actaaaaagg cagctacaag ctttagtata
    12001 aaaaatagag gcgaattaaa agaaggatat tttgcagata tagttatatt tgacaaggat
    12061 acagttagtg gttgtgatga ttttatcaac tctatgcagt accctacagg catagattac
    12121 gtaattataa atggaaattg tgttgttgaa gaaggaaaat acaataaaat taaagcagga
    12181 aaagtactta aaaattaaat cttagcttta aggaggaata aatttatgat tttagtgggg
    12241 aatgggaggc taataagcca agattcttta aatccctata tggaagatgg atgtgtagta
    12301 ataaatgata atctaattga agatataggt actacagaag aaatgaaagc taaatatcca
    12361 gtacatgaat ttatagatgc aggcaagaaa ataatcatgc caggtctaat aaattcacat
    12421 atgcatatat acagctcatt tgctagagga atggcagttc caggaaaacc ttctgagaac
    12481 tttttagaga tactaaacaa tttatggtgg agacttgaca aacagcttac actggaagat
    12541 acaaaataca gtgcatatgc aacgtatata gaatgtataa aaaatggtgt aacaacagtt
    12601 tatgaccatc atgcaagtcc aaatgcaatt gaaggcagtt tgtttacaat atcagatgtt
    12661 gcaaaggatt tgggaattag aacgtgtctt tgttatgaag tgtctgatag agatggtgga
    12721 aatacaatcc aacaaggtat caatgaaaac gtaaacttta taaaatacgc tcaaaaagat
    12781 gatacagaca tgataaaagg aatgtttggg cttcatgctg catttacact atctgatgat
    12841 acattaaata aatgtgcttc agagatggct ggtcttgata caggttacca tgtacatgtt
    12901 gctgaaggac tagcagactt acaatataat gtagataaat atggaaaaag ggttttagaa
    12961 agattcaatg attttggagt actaaaagaa aaaacaatag cagttcattg tatatatata
    13021 agtgtagaag aaatcagtct tttaaataaa acaaatacat ttgtagttca taatccagaa
    13081 tctaatatgg gaaatgcagt tggatgttct cctgcaatag agttattgaa aaggggagtt
    13141 acagtaggat taggaactga tggatatact agcgatatgt ttgaatcatt aaaagttgcc
    13201 aatataattc acaaacatca cttaaaagac ccaagagttg gatttgaaga atcacaaaca
    13261 atgttatttg ataacaatag aaaaatagct aaagcttatt ataaaaatga cctaggagta
    13321 cttaaaaaag gtgcttatgc agatgttata gtagttgatt atataccaca tactccattg
    13381 acagaaaata caataggggg acacactatt tttggaatga gtggaagaag cgtagataca
    13441 actattataa atggaaaagt acttatgaaa gatagaaagt taataaacat agatgaagaa
    13501 agtattctag caaaaagtag agaattaagt caaaatctat ggaatagaat ttaaataatt
    13561 gatattaata tacaagtaag aaacaagaat ttagatgtta aaaacagagc ttaaacaaaa
    13621 ataggaattt aaatgaagta tataaataaa aaatatacgg aatagaatat taaattaaat
    13681 atagaaggga gacccaaaat gtctcgagat aaaagcccta aaatagctcc agtagatgaa
    13741 aggttacctg tatccaaatc ttgggtattt tcactacagc acgttttagc aatgtgtgca
    13801 ggagcagtag cagttcctat gatgatagga agtgctgcac gactagatca tgctagtatt
    13861 gtttttttaa taaatgcttg cttgtttatg gcaggtatag gaacactcat acaaagtgtt
    13921 ggtataggaa atattgtagg tgctaaaata ccagtaatag aaggaactag ttttgccgct
    13981 gtggcaggta tgacagcaat agcctcatca tatagtgatt cacaagttgc aatacagaca
    14041 gtatttggct ctgtaatagt agctggattg ttttgtatat tacttgctcc agtatttgaa
    14101 aaattaataa gatttttccc tagtgtagtt actggaacag ttgttatggt aataggtatt
    14161 tcactatttc cagttgggat aaaatggata actgacaaca aattggaagc ggttgaacca
    14221 aactcattag cattagcttt agcagtattg gcaataactt tactttcatt taaattctta
    14281 aaaggaattt ggaatagcac agctatattg gtaggtatag tacttggtac actactttcc
    14341 atggcagttg gaatggcaga ttttagtgca gtaaatggag ttggttggct taacttaaat
    14401 gtacctttaa aatttggaat gcctagattt gaaccatctg caatactatc attgtgtttg
    14461 attatgttgg tacttatgac agaatcagtt ggtaatatga tagctattca tgaaatggta
    14521 gacaaagaag taacaggtaa aaatattaaa agaggtctat ttggagatgg aatatctaca
    14581 gcattatctg gaatattcaa ttcatttcca ataactccat ttggtcaaaa tacaggaata
    14641 gtaggtctta caggtataaa atcaagattt atagcagttt acgcaggggt tatattgcta
    14701 gtacttggat tcttaccaaa atttgcagca ataataggag caatacctaa gcctgttctt
    14761 ggaggcgtag gatttgcaat gtttggaatg gtaatggttg gaggagtaaa aactttaagc
    14821 aaagtgaaat ttgatggaac taaaaatggg gttatagtag cagttagtgt tggtttgtct
    14881 atgataccac taacaaatcc tgatttctat cataatttcc caacttgggt acaaacaata
    14941 ttccatagtg gaataacaac aggtagttta tcagcagtgc ttttaaacat attcttcaat
    15001 gaactaggtt ctaaaaaaga aaaaaataat aaagttgaag aagcaaaata aaaataaaac
    15061 aagtaaaaat ctatattgac ttaaagtcat taagtattaa taaaaataat tcagagatat
    15121 taataattca acaaaactat cttatatttt aaataatata aaaaagtaaa taaataacga
    15181 atacaaataa aagaatacaa tctaaatatt aaaaatattt aatttaaggg agagataata
    15241 atatggctaa tttaaaagtt aatttatatg ggatggaatt tttaaaccca attatgacag
    15301 cggcaggacc tggagcaaaa gatggtgctc tctgtcaaga ggcagtaaaa ggtggagcag
    15361 gagctatatg tacaaaaact atatctgtat taccagctga tgtaccaaga ccatgtatgg
    15421 caaaaactaa tagtggtttt ttaaacacag aattatggtc agagcttcca aaagagcaat
    15481 ggatagagga agaatacaaa aaggtaaaag aaacagggct tccagtaata gtaagtatgg
    15541 gatattcagc agagcaaata gctgaggttg ctcctttagt aaagccttat gcagatgcag
    15601 ttgaattatc tactcattat gtaggtacag acgttgcacc aatagtaaat gctttaaaag
    15661 ctgcaaaagc tgttttagat gtaccagtat ttatgaaaat gagtcctcat actgacattc
    15721 aaactattgc aaaagctgtt gaagaagcag gtgcagatgg acttgtaatg ataaactcat
    15781 ttggaccatg tatggcaata gatgtaaata caggataccc tataatggga agtaaagctg
    15841 gatacggatg gttatcaggt gcaccaataa gacctctagc aataagatgt atatatgaag
    15901 cttctcaagt tgtaaatata ccaataatcg gtgttggtgg aatcacttgt ggaaaagacg
    15961 tggctgaaat gatgatggct ggagcaagtg ctgttcaagt atgtacagaa gcaatcctta
    16021 aagggccttc tatctatgga aaaattgcta aagaattaaa tgaattttta gatgagaatg
    16081 gatatgctga tgttaatgaa ataaaaggat taactcataa gaaaatggca gctagagggt
    16141 ttagaactca ttcagtagct ccagtagtgg atgataacaa gtgtatcaaa tgtaagcaat
    16201 gtgtaacaag ctgtgtatac gaagctttag aagttacaga taagttgaac atagatgcag
    16261 acaagtgttt tggatgtgga ttatgtgtaa ctagatgtcc aaaaggagct ttaagcatct
    16321 gctacaatca agtagaagct atgaatgagt aataattata tagaattttg gtaattaact
    16381 tagagtaata ggtattagga taataaaata tatcagataa acaaacacac attatgcatt
    16441 aataatacag tctaaagtta ataattttat tatattgatt taactaaata ttagtttagc
    16501 tgaatattaa tttaattagg tattggttta atcttaatat agatttatta cgtataagtg
    16561 aactaactag attactttaa gttaattttt tcatataaaa taaattgata cattacaagt
    16621 aaggtggagt ctatatggga attgttttaa aaggtgggaa agtagtaaca agtgaaaaaa
    16681 cttactgtgc agatgtaaga atagaagatg aaaaaatagt cgatataggt cttgatatat
    16741 caaaagaaaa tgatgaaata gttgatgtta gtgattctta tgttatacca ggaggtatag
    16801 atacacacac acactttgac ttagatacag gcgttactaa gacagcagat aactttgaga
    16861 caggaacaaa agctgctata gtaggaggaa ctactactat attggatttt gctacacaag
    16921 ctaaaagatg ttctttgaaa gacggattag aagaatggca tgaaaaagct aaggataaat
    16981 cctattcaga ttatggcttt catatggcta taacagattg gaatgactcg gtttgtaatg
    17041 aaatggagga tatggtgaaa gagggagtat cttcatttaa actgtatatg gcgtataaag
    17101 gaagccttca agttgatgat ggtgtaatct ttgaagcatt gagaaaagca gaagagatag
    17161 gagggattat tggtttccat tgtgaaaatg gggacataat atgtgaactt gtggataaag
    17221 caaagtcaga aaataattta tctccaaaat atcatcaatt aactagacca gctataatgg
    17281 aggcagaagc tacatcaaga cttatgaaga tagctgaagt tgcaaactct aaagtatatg
    17341 tagttcactt aagctgtaaa gaatccttag aggaggtttt aaaagctaag caaagaggtg
    17401 tggatgtaat agttgaaact tgtcctcaat accttctatt aaatgatgaa ttatatgatt
    17461 tagatggatt tgaaagtgct aagtatgtaa tgtcaccacc tttaagaaat aaagaaaata
    17521 atgaaatatt atggaatgct attttagata atcaaataga cactatagga acagaccatt
    17581 gttcttttaa ttataaggga cagaaggaac ttgggataaa cgatttcagt aagataccaa
    17641 atggttctcc aggtgtagaa cataggatga gtcttttata cacatatgga gtctgtaaaa
    17701 ataaaatatc tatcaacaag atggtagaag taacatcaac taatgcagca aagttgtttg
    17761 gaatgtatcc taataaagga actatagaaa taggcagtga tgcagatata ttagtcttaa
    17821 atccaaacaa aaaatccaaa atagaagcaa aaaaacaatt acaaaatgta gattatacac
    17881 cttatgaagg gtatgaagtg gattgtcaaa tagaacatgt ttatttaaga ggaaaagaag
    17941 tagttagaga tggagaatta atttgtaata ctccaagtgg gagatatata aataggcgtc
    18001 taacaaaggg ggctagagaa tggtagaatt tatattaaat ggagagaaag ttgttgcaaa
    18061 agaaggcaaa aaacttttag acttccttag agaagatatg aatataactt cagttaaaaa
    18121 tggatgttca gaaggtgctt gtggaacatg tatggttata atagatggta aagctactaa
    18181 agcatgtgta tttaaaactg ataaattagc tgggaaaaac ataacaacta tagagggatt
    18241 atcagagaga gaaagagatg tttttgcata ttcttttgct agtcaaggag ctgtacaatg
    18301 tggattttgt attccaggta tggtaattag ttcaaaagca cttatagacg ttaataataa
    18361 tcctactgaa gatgatataa agaaagcttt aaaaggtaat atatgtagat gtacagggta
    18421 tgtaaaaata attaaagctg tcgatttagc ggcaaagatt tttagagaaa atatagatgt
    18481 gccaaaagta aactgtaagg gtctagtagg tgaaaatctt cctagagttg atgcagttgg
    18541 caaagcactt ggatattctg aatatgtaga tgatatgaga attgagggta tgatttatgg
    18601 ttcagcagtt agagctaagt atcctagagc tttagttaaa aaaatagata tagaagaagc
    18661 aaaagcttat ccaggagttg ttgcagttct aacagctaaa gatattccag gaactataaa
    18721 ggtagggcac ttaaagaagg attgggatac tttaatacca gaaggtgaaa taactagata
    18781 cttaggagat ggaatagttt tagtagcagc agaaactaga gaagcgttag aagaggccaa
    18841 aaagctagtt aagatagatt atgaggaatt aacaccatta agtactacag cagatgcact
    18901 tagagaagat gctccaaaga tacatgagac agggaatata cttgttaagg aacatttagt
    18961 aagaggaaac gctaaagaaa agatagaaaa ttcaaaatat gttgtaacta aaagatatac
    19021 tacacctgct acagaacacg catttttaga gcctgaatgt gcagtagctg gtccttatga
    19081 agatgatgga gtaataatct attcaacaga ccaaggggtg tttgccactc aacatgaatg
    19141 tgcagatatg ttagggttac catatgagaa ggtaagagtt ataaacaaaa tggtaggagg
    19201 agggtttggt ggaaaagaag acatgagtgt tcaacaccat gcagccttac ttgcatacca
    19261 tacaaagaga tttgtaaaag tacagttaac aagaaaagaa agtattattg ttcatccaaa
    19321 acgacatgcc acagaaatgg aatttacgac agcatgtgat gaaaatggat acttaacggg
    19381 tatgaaggca acgataatat ctgacacagg tgcatatgcc tctcttggag gaccagtgct
    19441 acaaagactg tgtactcatg cagcaggacc atacaattat caagatattg atataacagg
    19501 aacagcagta tatactaata accctcctgg aggagcattt agaggatttg gagttacaca
    19561 gagttgtttt gcaactgaat gtaatctaaa tttacttgct gaaatggttg gaatatctcc
    19621 ttgggagata agatataaaa atgcaataag acctgggcaa gttttaccaa atggtcaatt
    19681 tgcagatgaa ggaacagcaa tggcagagac actagaagct gtaaaagaag agtatgaaaa
    19741 gcatccacgt gcaggtatag cttgtgctat gaaaaatagt ggggttggag ttggtatacc
    19801 tgatgttgga agatgtagat tggtgataaa agatggcaaa gttcacataa gaacaagtgc
    19861 ttcttgtata ggtcaagggc ttggtactgt tatgactcaa gtagtttgtg aaactgctaa
    19921 cctacttcct gaaaatatag ttcatgaagt tccagatact catttaacac cagactctgg
    19981 taatactacg gcttcaagac agacagtatt tacgggagaa gctactaagg ttgcatcaaa
    20041 taagttaaaa gaagctttag actctggcaa aactttactt gatttagagg gagaagagtt
    20101 ttatggagaa tatgaaagta ttacagataa aatgggttgt gacaaagaaa atccagtaag
    20161 tcatgttgct tatggatatg caacacaagt tgtaattctg gatgaaaatg gaaaactaga
    20221 aaaagtagta gcagcacatg atgttggaag agctataaat ccaatcagtt tagaaggtca
    20281 aatagaaggt ggagtagtta tgggtcttgg atatgctcta accgaagatt acccagtagt
    20341 taactctatt cctacagcta agtttggtac attaggttta tttaaatcta cagatatacc
    20401 agaaatacaa gctttaatag ttgaaaagaa caaagattta ttggcttatg gggcaaaagg
    20461 tgtaggagaa atttgtacag ttcctactgc accagcagta caaaatgcct attatgtatt
    20521 tgataaagaa tttagaactg agttaccact acaaaatact ccatatagta aaaaaaagta
    20581 acaaaaaaaa gaaatattaa ataaaaaaat agatgaaatt gtaaataaaa aaaacccttt
    20641 tgcataaggt atattttaaa tgatagaata tacatataca ggagggtttt tcttttataa
    20701 aataagttta ataaaattga gaggtgagca gaatgaattt taataatagt ctagaattaa
    20761 ctcagtctca gaagctaata atgactacac aacttaagca atcattatca atattaaata
    20821 tgagtaagtt agagttagaa gaagagataa aaagagaagc agagaataac ccattagttg
    20881 aagtcgagaa aagtggggag ataaattggg aagagtatat aaaagacatg gataggtcaa
    20941 gacgtttaga tagaacagag ataagctata atcctgataa tgaagttaat ctagaaaatt
    21001 tagttaaata ttcgtcaaat ttatatgaag atttaaaatt tcagataagc ctgtataaac
    21061 ttacagacaa agaattggaa gtctgtgagt atataattga tagtttagat gaggatggat
    21121 atttaagaac tgaggaaaaa gtaattgttg ataccttaaa gatagatgaa gaactttttg
    21181 aaaaatgttt aattagtgtt cagcagttgg aacctagtgg agtaggggca agaagtcttt
    21241 cagaatgtct gatgatacaa atggctagtt taggaatata taatgaaatt ttagaggaaa
    21301 tcgttacaaa agacttaaat ttaattgcaa ataataaata caaagagata agtaaaaagt
    21361 acaatatgtc attacaaaaa tgtgttgaac taataaacat aattaagaca ttagacccca
    21421 aaccaggaag aacttgttca gtagagaaaa gtgtatacat tcaacctgat gtaacagtag
    21481 aaaaaattga tgatgaattt attgtatatt taaatgagaa agattcttat caaataagaa
    21541 tcaatagtta ttataaagac atattaaaaa attcacagtc tgatgaaagt gcaaaagaat
    21601 ttataaaaga aagactaaat tcagcaacag gacttatgaa aaatatagag agtaggaaaa
    21661 ctacagtact taagattgca gaagagatag taaaagctca agatgaattt ttgagaaaag
    21721 ggacaaaata tataaaacct ttgaaaatga aagacatagc agaaaaatta gaatttcatg
    21781 aatctactat aagtagaggt gtaaatggta aatatatgct aacacctttt ggcgtatatg
    21841 aatttaggta ctttttcagt agcgccattg agactgagaa taatgaaatg gcatctagta
    21901 caagcataaa aaaaattata aaggaaacaa taaaagacga aaataaaaag aaacctttaa
    21961 gtgatgacca tatatctaag ttattaaaag aaaaaggaat caatgttgca agaaggacag
    22021 ttgccaaata tcgtgaagaa ttaggaatcc catcttcaag taagagaaaa gaatactaat
    22081 aagtaaaaga caaatatcag tatggtaagg ggaaaaggct aattaagaga tgaatgtaga
    22141 ttgggctaaa aattttataa agtaaaataa tataatatga aaaagtatta aaatgctata
    22201 aggtattaaa aaactgggga acattaaaac attaagatag caaagaacat aaaaaactaa
    22261 agaagattaa aacattaaag aacattagag ctttgaggaa tattagaact ttaaagaata
    22321 ttaaaattta taaaatataa aaatactaaa aagaattgaa aaactgggga acattaaaat
    22381 attgtaaaat gctaaaaaac tagaaaacat aattaaataa gtgcttataa agaatgatta
    22441 aattaaataa gtgtttgtaa agaataatta gatattagaa aaagaagaat acctagataa
    22501 ataaactatt attaaattta ataatcacaa aaggaaaaca cgtagggtat aaataaaaat
    22561 cttaactaga taaaagtaaa cttagttgta gataaaaata actagaaata aaattattta
    22621 tagtaataaa tattagaatg aaaatataaa aaaatatata taaaaagcct tatataaaaa
    22681 gaaaaaatac tgtgatttga aaatggtgga gaaatatttt ataaaataaa aaaataaaaa
    22741 agtttttgaa aaactattga aatctatacg caaatcctat aaaataaaaa tatcttgtgg
    22801 gacttaaata aacactaggg acaaaaaaag tcccgaaagg aaagcaataa ggagtatgtt
    22861 atgaagaact tattagaaat tcaaaagaaa ttgattccac aggcaattga acttatggaa
    22921 agaagatact ctattttaag acaaatatct ttaagtgaac caataggtag gagaacatta
    22981 tcaaatgtat tagagattag tgaaagaata atcaggtcag aaactgagtt tttaaaagaa
    23041 caaggactta tagacgtagc tgtatctggg atgactatca ctaaagaagg ttctgatttg
    23101 ttagataaac ttaaggacat catgagtgac atcatgggat tgtctaagtt acaagataga
    23161 gtcagagaaa agcttggaat aaaaagggta atattagttc ctggaagctg tgatgaaaat
    23221 ggaagtttac taaaagatgt aactagatat ggatgcgaat atttcttagg cattttaaaa
    23281 gatggagata tcgtttcaat aacaggagga agcactatgt tggagttttc aaacgttatt
    23341 aaaactgata agagatatcc aaactctaca atagttccag ctagaggaag tatgggaact
    23401 gatgtagaga tgcaatctaa caatatagtt gcttcaataa gtaagaaatt acattctaat
    23461 tgcaaacttt tacatatacc agatgagttg gatgaaagtg caatgaaaac tcttagccaa
    23521 gtgccagaga ttaagacaac tttggagtat attaaaagaa cagacatttt agtttttagt
    23581 ataggtagag cagatgtcat ggcaaagaga agaaaacttc cagaagacaa ggttgatgag
    23641 atattatcta aaaatgcagt tggagaagca tttggatatt atttcaacaa agatggagaa
    23701 atagtttaca aactgaacac tgttggaatt gatttagaaa cagttaaaca agtaaaagaa
    23761 actattgcaa tctttgcagg gactgaaaaa gtagaagcct tattagctat atcaaatatg
    23821 cataaaaaca tggttcttgt aacagatgaa gaaagtgctt ataagatatt gtcattatta
    23881 taaaagtcaa gacctagcac agagctagaa aacatacatt tactttatag gaggtagcga
    23941 aaatggttaa agtagctata aatggatttg gaagaatagg aagattagca ttaagaaaaa
    24001 tgatggagca acaagataag ttcgaagttg ttgcaataaa cgacttaaca gacgctaaaa
    24061 tgttagctca tttatttaaa tatgatacag ctcaaggaag atttaacgga gaaatagaag
    24121 ttaaagaagg tgcttttgta gtaaacggaa aagaaataaa agtaacagca gaaagaaatc
    24181 ctgctgacct accatgggct gaattaggag tagatatagt attagaatgt acaggattct
    24241 ttacatctaa agataaagct gaagctcata tacaagcagg ggctaagaaa gttgttatat
    24301 ctgcaccagc tactggagat ttaaaaacaa tagtattcaa cacaaacagt gacatattag
    24361 atggttctga aacagttata tcaggagctt cttgtactac taactgttta gctccaatgg
    24421 ctaaagtatt aaatgataaa tacggtatag aaaaaggatt aatgactact atacatgcat
    24481 acactaatga ccaaaatact ttagatggtc ctcatccaaa aggtgattta agaagagcta
    24541 gagctgctgc tggaaacata gttccaaaca ctacaggagc tgcaaaagca ataggcttag
    24601 taataccttc attaaaagga aaattagatg gggcagcaca aagagtacca gttgtaactg
    24661 gttcaataac tgaattagtt tgtacattag gaaaaaatgt tacagttgaa gaaataaatg
    24721 cagctatgaa agaagcttca aacgaatctt tcggatatac tgaagaaatg ttagtatctt
    24781 ctgacataat cggaataagc tacggttcat tatttgacgc tactcaaaca aaagttatgg
    24841 aagtagatgg aaaacaatta gttaaagttg tttcttggta tgataatgaa atgtcttata
    24901 cttctcaatt aataagaact ttaggatact ttgcacaatt agcaaaatag tcagttttga
    24961 gaaaatagaa aagtagattg acaaagtccg agtttgtagt ttaatactac ggactcagga
    25021 ctttttttaa gaaaaaatat ataaaataca cctaaataat gttacatact atatataaaa
    25081 atatgataat atgatatatt taatagggta aaagtatgta ctatattata cttggcaata
    25141 aaaaattact ttattaaaag gagagatatt tatgtcaatg cttaacaaaa aaactataga
    25201 agatattgat gtgtgcggaa aaaaagtatt agtaagatgt gactttaatg tgccattaca
    25261 agatggtgta attactgatg aaaatagatt gaacggagct ttaccaacta ttcaatattt
    25321 aatttctaaa ggggcaaaag ttatattatg ctctcactta ggaaagccaa aaggagaagc
    25381 taaaccagaa ttatctttag caccagtagc aaagagatta tctgagatgt taggaaaaga
    25441 agttgtattt gcagcagacg ataatgttgt aggagaaaat gcaaagaaag caactgaaaa
    25501 aatggaaaac ggagatgtag tattattaga aaatactaga tatagaaaag aagaaactaa
    25561 aaacgaagag aatttctcta aagaactagc atcacttgct gaaatatttg taaatgatgc
    25621 atttggaact gctcatagag cacattgctc aacagttggt gcaggcgagt ttttacaaga
    25681 gagagtttgt ggatacttaa ttcaaaaaga attaaaattc ttaggagaag ctgttgcaaa
    25741 ccctgtaaga ccatttacag ctatacttgg aggagctaaa gtttctgata aattagctgt
    25801 tataaacgaa ttattagaaa aagttgataa tctaataata ggtggaggaa tggcttatac
    25861 attcttaaaa gctcagggtt atgaagtagg aacttcttta cttgaaatag ataaagttga
    25921 atatgctaaa gaaatgatgg aaaaagctaa gaataagggt gttaacttat tattaccagt
    25981 agacgttgtt atggcagacc actttgcacc agatgctact ccaatagtaa cagaagatgc
    26041 taatgtaaaa gaagattaca tgggactaga tatgggacct aagactatag caaactttgt
    26101 aaaaactata aaagaatcaa aaactgtagt atggaatggg ccaatgggag tatttgaatt
    26161 tgaaaacttt gcaaatggta cattatcagt agctagagct atggcagagt tgactgatgc
    26221 aactactgta attggcggtg gagatagtgc agcagcagtt aatcaattag gatttggtga
    26281 caagatgact cacgtatcaa caggtggagg agcatcacta gaatttttag aaggtaaaga
    26341 attaccaggt attgcagcat tagataataa ataaactaat atatagagga gattgtaaaa
    26401 tgagaaaaac tataattgca ggaaactgga aaatgcataa aacaataaaa gaagctttag
    26461 aatttgtaaa tgaagttaaa gataaagtaa attctgataa agtagaagct gttatatgtg
    26521 caccatttac tttattaaag gatttaaaag aagctactaa gggtacaaat ataaagatag
    26581 gtgctcaaaa tatgcacttt gaagaaaaag gagcatttac aggagaagtt tcacctctta
    26641 tgttaaaaga aatagatatg gactatgttg taataggaca ttcagaaaga agacaatact
    26701 tcaatgaaac tgatgaaact gtaaacaaga aagttttaaa agcattagaa gtaggaatag
    26761 acccaatatt atgtgttgga gaaactttag aacaaagaga agctggaaaa actaaagatg
    26821 tttgtaaagt tcaagttgaa aaagctttag aaaatgttct taaagatgat ttagcaaaag
    26881 tagttgttgc atatgagcca atctgggcta ttggaactgg taaaactgca actgctgaag
    26941 atgctaatga cgttatatct tatataagag aagttataaa aggattatat ggagaattag
    27001 ctaatgaagt tagaatacaa tacggtggaa gtgttaaacc ttcaaatgta gccgaaataa
    27061 tgggtcaaag tgatatagat ggagctttag taggtggagc tagtttagct agtaatgatt
    27121 acctagacct tgttaatttc taaggagtga aatgatgaaa aaaccagttg ctttaattat
    27181 tatggacggg tttggataca ataaggatgt taagggaaat gcaattgctg agtccaaaac
    27241 tcctaattta gatagaataa aaaaagagta tccaaacaca ttgataaatg ctagtggtct
    27301 tgatgttggt ctacctgatg gtcaaatggg aaattcagaa gttggacaca ctaacatagg
    27361 tgcaggtaga atagtatatc aagatttaac tagaataaca aaatctataa aagatggtga
    27421 cttttttact aataaagttt tatgtgaagc aatggataat gcaaaagaaa attctcttca
    27481 tgtaatggga cttttgtctg atggaggagt acattcacat atagaccatc ttaaggctat
    27541 aatcaaaatg gcaaaagata aaggcgtaca aaaggtgtat gttcacgcat ttactgatgg
    27601 tagagatact gaccctcaaa gtgcattaga atatgccaaa gaagtacaag ctagtatgga
    27661 tgaaattgga gtaggtgaat ttgctacagt ttctggtaga tactacgcta tggatagaga
    27721 taagagatgg gaaagagtag aacttgcata caatgccatg gttagaggta ttggtgaaaa
    27781 agcaaattct attgaggaag ctattcaaaa ctcttatgat gatggaaaaa atgatgagtt
    27841 tataatgcca acagttatta tgaaagatga taaaccagct ggaagtataa aggaaaatga
    27901 ttcaattata ttcttcaact ttagaccaga tagagctaga caaataacta gagctttagt
    27961 ttgtgaagag tttgatggat ttaaaagaga agatattaaa aacttctttg tatgcttaac
    28021 agagtacgat ataactatag agaatgtgca tatagcattt ggacctcaat ctttagccaa
    28081 cactctaggt gagtatctag ctaaaaatgg aaaaactcaa cttagagctg ctgaaactga
    28141 aaagtatgca catgtaactt tcttcttcaa tggtggagta gaagagccaa ataaaggtga
    28201 agaaagacta ttgatacctt ctccaaaggt tgcaacttat gatttaaaac cagaaatgtc
    28261 agcatatgaa ttgacagata aagctttaga taaattagga gaagataagt ttgactttat
    28321 agtattaaac tttgcaaatc ctgatatggt aggtcataca ggaagtatag aagcggctat
    28381 aaaggctgtt gaaactgtag atacttgtgt aggaaaattg atagataaaa tagtagagtt
    28441 gggtggaagt gcaataataa ctgctgacca cggaaatgct gaatatatgt tagacccaga
    28501 aacaggaaaa actgtaacgg ctcactctat taatccagta ccatttattg tagttggtca
    28561 agaatatgaa agtgcaaaat tattggatgg aggaagatta tctgatatag caccgactgt
    28621 tttagatatg atgaagttag aaaagccaga agaaatgact ggtcattcac ttatttcaaa
    28681 atagtcgatt ttgataagta gagaataata tataaactac aatataaatt taaaaataat
    28741 gcagtttagg tttattttgt aaagtttaga ctttatgaca taagatttaa attttgtaag
    28801 ttctggatat tgtaaggtgt agatgaagat atattttata aaatttacaa gatagacaat
    28861 ttataaaata aaaaattaca atatagataa taatgtaaat aatgatataa ataataacaa
    28921 gatagacaat aa
//
LOCUS       DAGSHT010000030        28148 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGSHT010000030 DAGSHT010000000
VERSION     DAGSHT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.031"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(232..1344)
                     /locus_tag="MJB72_001205"
     CDS             complement(232..1344)
                     /locus_tag="MJB72_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY8262330.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(1470..2474)
                     /locus_tag="MJB72_001206"
     CDS             complement(1470..2474)
                     /locus_tag="MJB72_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY8262331.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIRDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSV
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(2881..3729)
                     /locus_tag="MJB72_001207"
     CDS             complement(2881..3729)
                     /locus_tag="MJB72_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY8262332.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(3765..4235)
                     /gene="trmL"
                     /locus_tag="MJB72_001208"
     CDS             complement(3765..4235)
                     /gene="trmL"
                     /locus_tag="MJB72_001208"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBY8262333.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(4429..5481)
                     /gene="nth"
                     /locus_tag="MJB72_001209"
     CDS             complement(4429..5481)
                     /gene="nth"
                     /locus_tag="MJB72_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBY8262334.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMETIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            complement(5678..7915)
                     /locus_tag="MJB72_001210"
     CDS             complement(5678..7915)
                     /locus_tag="MJB72_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8262335.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(8021..8935)
                     /gene="queG"
                     /locus_tag="MJB72_001211"
     CDS             complement(8021..8935)
                     /gene="queG"
                     /locus_tag="MJB72_001211"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBY8262336.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            10032..10436
                     /locus_tag="MJB72_001212"
     CDS             10032..10436
                     /locus_tag="MJB72_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBY8262337.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(10693..11688)
                     /locus_tag="MJB72_001213"
     CDS             complement(10693..11688)
                     /locus_tag="MJB72_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY8262338.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(11972..12808)
                     /locus_tag="MJB72_001214"
     CDS             complement(11972..12808)
                     /locus_tag="MJB72_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBY8262339.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(13296..13859)
                     /locus_tag="MJB72_001215"
     CDS             complement(13296..13859)
                     /locus_tag="MJB72_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262340.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(13957..14886)
                     /gene="glsA"
                     /locus_tag="MJB72_001216"
     CDS             complement(13957..14886)
                     /gene="glsA"
                     /locus_tag="MJB72_001216"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBY8262341.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(15757..17424)
                     /locus_tag="MJB72_001217"
     CDS             complement(15757..17424)
                     /locus_tag="MJB72_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBY8262342.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(17498..18253)
                     /locus_tag="MJB72_001218"
     CDS             complement(17498..18253)
                     /locus_tag="MJB72_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBY8262343.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(18263..18799)
                     /gene="lepB"
                     /locus_tag="MJB72_001219"
     CDS             complement(18263..18799)
                     /gene="lepB"
                     /locus_tag="MJB72_001219"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY8262344.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(18965..19501)
                     /gene="lepB"
                     /locus_tag="MJB72_001220"
     CDS             complement(18965..19501)
                     /gene="lepB"
                     /locus_tag="MJB72_001220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY8262345.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(19601..20911)
                     /locus_tag="MJB72_001221"
     CDS             complement(19601..20911)
                     /locus_tag="MJB72_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBY8262346.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(20990..21706)
                     /locus_tag="MJB72_001222"
     CDS             complement(20990..21706)
                     /locus_tag="MJB72_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBY8262347.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            21951..23222
                     /gene="sleC"
                     /locus_tag="MJB72_001223"
     CDS             21951..23222
                     /gene="sleC"
                     /locus_tag="MJB72_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBY8262348.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(23699..24355)
                     /locus_tag="MJB72_001224"
     CDS             complement(23699..24355)
                     /locus_tag="MJB72_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262349.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKIMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(25709..26119)
                     /locus_tag="MJB72_001225"
     CDS             complement(25709..26119)
                     /locus_tag="MJB72_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262350.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYVAID"
     gene            complement(26335..27717)
                     /locus_tag="MJB72_001226"
     CDS             complement(26335..27717)
                     /locus_tag="MJB72_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY8262351.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSSIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(27728..28087)
                     /locus_tag="MJB72_001227"
     CDS             complement(27728..28087)
                     /locus_tag="MJB72_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8262352.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
BASE COUNT         9072 a         5213 c         2640 g        11223 t
ORIGIN      
        1 tatagctttg tacagttggt tcaaatttaa taaatatatc tttttatcac taatctaaac
       61 ttaagcagta tattccgtac agttagcaca aatttaataa atatatcttt ttattgctaa
      121 cctaaactca atcaatatat tttgtacagt tagactaaat ttaataaata tatcctttta
      181 tcaccaatct aaacttaagc agtatatttt tataatcttc tacatcaata attaatatgt
      241 atctttcact ttgttatact ctttttttat gtattccaaa acatctggtg gtatatttct
      301 actgctcttt gaagtcaagc caagttctcc atcttttatt ttaaattcca ttactttact
      361 tcccttaaaa ccatcttttg caacgttctt tatcagttca tatacaggtt gttctacatc
      421 ttttataatt gaacttatta cattatctgg agccatatcg tattggtctt tattaatagc
      481 tataacttta ccaccatttt ctcttgctac ctgaatagct cttttgctat catcctctgt
      541 agctgaaaat acaacatctc tatcagaatc tctcatttcc tttgcaatag ttctagctga
      601 ttcagggttt tcaaatatat ttgaatactt aactagtaac tcagcatctt tatttgaagt
      661 tttaagcccc tttctaaatc cagattcata cttatctcta ctagaaccct caactcctcc
      721 tataaaaccg actttcccag tatcagtcat tttacctgca ataagtcctg ctaaatatcc
      781 agtaacatta tcttgaaatg atacagaaat tacattagct ggctgaactt tataagtata
      841 gtcaactatt gcaaattgct gtttgggata tttagtagca gcttctttaa ttggctctgc
      901 aaatctggct ccaactgcta ttatcaaatc tggttcatac ttatgcagtt tttctatact
      961 ctctttaaaa cttttagaag tctttggttt ttcaagtttt atcctaacat caaaatcttc
     1021 gtcagccctt tttaaggctg atagtacatt gttattatat gattcgttat ttaagttttc
     1081 ggtatctaac aatactccaa tagtttttct tacatatgaa tcatcttctg tgtcatcttc
     1141 tttattctta cttttgctat ttgatttatc cttttctgct gaagttggca aacttttaga
     1201 ctcattctgt ttcgatttag cactatctat attagaatta ttcttattta ctttatcaaa
     1261 tccaaaatca aaattacata tagctaatat aaaaattgta aaaactacta caaagcaagc
     1321 catcaatatc ctttttattt tcacaaaatt cactccctga tttagatagg tctaacctac
     1381 ctctgcatat gtctttctat atatgtctat tataacatcc atttcacctc ataactaaag
     1441 tcataagtat tcttggatat tatagtagat tatttattgt tcttattaac atattctaac
     1501 acatctggag gaatattttg agaactattt tgggcaagcc cagtacttcc agacttaact
     1561 gtgttttcta taacttttcc gcctttataa tttcctttta caaagctttg tatcagatta
     1621 taagtaggat tctcaaaatc ttttacaaca gaacttataa cattttctgg agctaactca
     1681 tgttgatctt tattcgttgc tataactttt ttattttttg cccttactgc atctattaca
     1741 gcttttgaat catcttcagt agtagaaaaa ataatatcca ctccattatc catcattttt
     1801 ttagctatag attctactga tttgctatct ttaaaaacat ctgcatactc aactgaaata
     1861 tcccttatac tactattaga aaatttaaca ccctctctaa aacctgattc aaatttatcc
     1921 ctactagaac tctttgttcc tcctataaaa ccaaccttat cgctttcagt catttttcca
     1981 gcaattaatc cagctaggta tccagattta ttatcttcat aagaaattga agttatatta
     2041 gaaggttgct tgtcatactc atagtctata attgcaaatt gttgctttgg atatttcttg
     2101 gctaccatct ccaatggttt tgcaaatcta gctcctacag ctataattaa atcgtaattg
     2161 tcattacata aaatatctat actattagaa aaagttgagg aatcatctga ttccactttg
     2221 tttgtatcta tattaagttt tttttgagca ttgtttaatg ctaataaagc tgaattattc
     2281 atagcttcat tatttgttcc ttcaatatct agtatcattg atacagattt ttttgaatta
     2341 cttttatcgc ttatatcact tttattatct ttgctagagc taggattatt gcttgggtta
     2401 ttagcacacc caaccaacat tccagctatc attgctacag ttaacatcaa tatcaatatt
     2461 cttttgaaac gcattaaatt tcactcctca tacatttacg atatttttat atcctattta
     2521 cttttacatc ctatttactt ttacatccta tttactttta catcctattt acttttacat
     2581 cctatttact tttacatcct atttactttt acatcctatt tacttttaca tcctatttac
     2641 ttttacatcc tatttataat ttttttatat acaatttaca agtacatttt gaaatttttt
     2701 taataaatct tactataaaa aagtgtgcaa tagattctta tctatttaca cacttcccta
     2761 caaactaaat ctaatatatc aacacttaat ttcatccaaa ataaatccaa tttaacatct
     2821 caataattaa tattctatcc taatttaata aaaaaataaa taatgtcatc caaaaatata
     2881 ttatttattt tttaagcata tgataccagt aacaccagga ccagcatgtg cacctataca
     2941 cacaccaatt tggaaaaatc taatttcttt aggtcttaat tcatcttgca taaccttaga
     3001 taattgttcc ttttccttaa aatcatcact atatcctata tacacaattt ggtctgataa
     3061 atcatcacca caatttgatt ttatcaattc catcatctta gaaattacat tcttctttcc
     3121 tcttacttga gatatttgcg aaactaatcc atctcttatt tcaagaatcg gttttatatt
     3181 taatatactt cctattgctg ccttagtaga tgatattcta cctccctttt gtaaatattc
     3241 caatgtatct actgaaaata taacaaaagc cttctctttt aattcatcca aaacaggcaa
     3301 tatttcagca acagactttc cttcacttgc aagttctcct gcttttatta caagtaaacc
     3361 agttcccata caaagcaagt tagagtcata tgtatatatt ttaccatcta catcattctt
     3421 tgccattaca gcactttgat acgtacctgt agctgtggca gaccctgcta tataaagtat
     3481 ctctctatcc tcatttgtat atttctcaaa aatttcctta aattgggcat aagtagcttg
     3541 agaagttttt ggatatacat tttcttctct aagcctttta taaaattcat ctccagatat
     3601 atcaattcta tccttgtatt ctctttcctc cagtataact gttaatgaaa ctatatctat
     3661 atcatattta tctaacaaat cttttggcac gtccgataaa ctgtcacaga ttatctttag
     3721 atttttcatt aagtatgctc ctttctcatt taggcttgtt aaaactacct catatttggg
     3781 tatcctattt gtcttaaagc ctcgtacact acaatagcaa ctgaatttga taaatttaat
     3841 gacctagctt tttcaacatc caacataggc actctaatac aagtatccat atttgctttc
     3901 aacaaaggtt ctggtaatcc ctttgtttct ttcccaaata ctataaaaca attttcttca
     3961 tatttcacat ctgaatgtga ctgtttagcc tttgttgtag aaaaaaagta tttactattt
     4021 ggatactttt cttgtaattc ttcaaagcta tcgtaatatt gtatatcagc tatatcccaa
     4081 tagtcaagcc cacatctttt taggtgttta tcatccaatg aaaaacccag tggttttact
     4141 aaatgtaaag ttgaacctgt tgctgcacag gttcttatta tatttcctgt attttgaggt
     4201 atttctggtt ctactaatac tatatttaat gacattttaa tcctccctaa gtattgcttt
     4261 tatttatcaa attttatttt ataaacagaa taacaataat attatactat tatataaaat
     4321 aatgttaata ataattttta tttcatgtat ataatagttt tttatctaac attttcacaa
     4381 gtattattat atcagaaaat ccctcttgta caacttactt tctaaaagtt atttcgtttc
     4441 attaagttct ttataataat tacaatcttc ttttattggg caacttgcac attctggatt
     4501 tctagcttta cacattcttc ttccatgaaa aattaataca tgatgtgagt gtgaccatct
     4561 ttcttttggt atagtttcca taagtgcaaa ttctgttttt tgaggatttg gctcgtccac
     4621 tattcctatt ctattgctaa ctctaaatac atgagtatct acagctattg ctggatgatt
     4681 aaaagcgtta ctaagtacaa cccctgccgt ttttcttcca acaccaggta attttataag
     4741 cttttctaag ctatcaggta cttcaccatc atataattca catagctgtt cagatgtatc
     4801 ctttattttt tgagacttgc ttttgtaaag accacaactc tttatctctt tactaatttc
     4861 ttcaatgctc aaattagcaa agtctctagc tgtgttgtac tttttaaaca actctgaagt
     4921 aactttattc actcttacat cagtacattg tgctgataaa atagttgcta ttaaaagttc
     4981 aaaggctgtc ccataattta actcacattt agcatctgga tacaattttt ctaactcgtc
     5041 taaaatctta tttacgtctt tttcatttgc tgactcattt ttaattttag acttactttt
     5101 agtttcttta cttgtttttt gtgtagcctt attagaacta ctcttctttg tttttttctc
     5161 cgtttcacct tttttagtac tttttttact tactttgata ctttttttat cttcttcaat
     5221 atccataaaa ttctctatat ctttctcatc aattatctca tcttttttac caatcatttt
     5281 atctattcct ttttgaacct cgaccttctt tacatttttt ttatctatct cttctttctc
     5341 aattatatct ttcttatcta tacttttttt atttacttct tccttctcaa ttacatcttt
     5401 cttatccatt ccttttagat ttatatcttc ttccacaatt acatcttttt catttatctc
     5461 ttttagattt acatttttca taattatccc cctttaaaat actaatttat aaaacgctat
     5521 ttataaatag tttaatcata tcaacaaaaa ataaacatca atatataaaa catacaaaat
     5581 aagaatttaa tttatatata tttacaaaaa aagcaaaaac ctattaaagt tcttgctttt
     5641 tattatacat aaatacccat taaattctat aataaactta ttcttttgac acagtttgta
     5701 tcttgctgaa agatattaat ttttctttta ccaatttctt tatttcttca aatttaacat
     5761 ctgtcaatat ctctatagcc tcttctactc tttcaacagt atatatctta aattttccat
     5821 ctcgcactgc atcattaact tcatctgata aaacgagatt tctttgattt tttctaggaa
     5881 ttatgacacc ttgctcacca tttaaccctt tagttttaca tatagaataa aatccttcta
     5941 tcttttcact tataccacca actacttgta tatcaccctt ttgatttata gaacctgtaa
     6001 cagcaatatt ttgtttaatt gatactccac taagagatga taatagtaca tataattctg
     6061 cagaagatgc actatctcca tctattccac cataattttg ttcaaaacaa acatatgcat
     6121 ttagtgacaa tagtaaatcc tgtgcaaaat tctcagaaag atagccacct aatataagta
     6181 ctcctttgtt atgtatagag ccactcatat ttacttctct ttctatattt actatgcctt
     6241 tacttcctgg gctagtggtc gctgtaattc ttgaagctct accaaaagag tattctcctg
     6301 tacttaagac tgacagacca tttatcacgc ctacttttct tccctctgtt tctattagag
     6361 taaatccatt ttcaattgat tcatccatat gtgtttccac tctattgatt ctttttctct
     6421 tttcacttat tgctttttta acatcacact gctctgtata ctccgaattt ctaaactctg
     6481 cataagcact tccttctatt acaacttcca ttattttatt aaacttagtt gatagttttt
     6541 ctaaatcacc agataatcta gtgctaaatt taataacctc ttcaactgct gatttagtaa
     6601 aatgccttaa attattctta tcacattggt atgctataaa tcttgctatt ccttcttcat
     6661 tatcttcatt tttatccatt tcactatcaa agtctacaaa tactttaaaa tatttttcaa
     6721 aatcttcatc atatctatat aaaatatcat atatataatg gcttccaatc agtactaatt
     6781 taacatccaa tggcatattt tctggtttaa ttgctgtttg tgtttccaat attacttttt
     6841 tagtctgcaa agtcttttta agaatttccc aagacattgt atattttagt aattggtctg
     6901 cataaagtac taaataacca ccattagccc tatgaaatgc tcctgataat agttttgtga
     6961 aatcagtctt tatatttcca tttgcataat catactctgc ctttccaaat agatttgctg
     7021 gacttgggtt tatctctact acaacaggtg ctgattcttt attctttgag cttccattgt
     7081 ctacaaatag gttaatttta tatttaataa aatgttcttt atcatactta tcttttaact
     7141 cctcttcatc taaatagaat aaatacatat attctattat atctttcttt atattttcca
     7201 gataagtttg aatcttatca taattttcat atttttcaca caatctcttg atatgaggtt
     7261 ctactacaag tttagctatc tcttcttcaa gctctaacac tgctttttca gctatttctt
     7321 ctaagtttct aattttatat acgacctgta tcgccatatt ttctagttct cttttagctt
     7381 tgtaaaactc ttcatcagac tcatcacttt catcttcctc ttctttcaaa gggataaaaa
     7441 ctattcccat tttactctgt tttaatttaa atcctttttc ttctccatat ttcttaattt
     7501 gcttcaaaag catatctttt tctatttcat attcatccag aagtttattt ttttctattt
     7561 caaaattttc actttcaaac gcatttttaa aatcttctaa aagactttca attaatttct
     7621 ccatatcttt tttaagttct cttccaaacc ccttttctaa ttcaactata ataggctctc
     7681 tagggttttc aaaattatat atataacacc aatctttatg cgtatttttt ttggatgcat
     7741 actcatttaa aacctttaag gcataagtac ttttaccagt tccaggttca ccagctatat
     7801 atatgttgta cgctggatta tttattttca accctaattc catagcttct acagctcttt
     7861 cttgacccaa tatataatct agtggattta tttcagatgt attattatat ttcaatattc
     7921 ccccacctcc ccatatttaa ctttaatgat ataatatgac ttttacacaa caagtgttaa
     7981 agtaaataca cattaaagta tatgaaacag actcttatta ttagtcatca tacttatcta
     8041 aaattatttc catatttctc tttattatat ttctccctct ccagctaaaa gctctatctc
     8101 catatctcct tttaaattct ttatttgaaa tattttgtat ctcatcataa tccaaatagt
     8161 ctattatatt ttctttaaat tccaaaatat gagttgttgg tatttcaaca ttatgtggac
     8221 atacttcttg acaaatatcg catccaaata cctttttgcc attctttaaa acaacttttt
     8281 ctttctctga caaatctcct tttttttgag ttatatatga taaacatttt ttggggttca
     8341 tatcataatt tcccaatata gcatttcccg gacaatattt tacacactta ccacatttaa
     8401 aacaactttt ttctagtggt ttatcttctt caaatttata attatttact atatacccta
     8461 taaatacgta agaaccatat tcatcagtta tgatattatt atttattcca aaatatccta
     8521 ttcctgataa ataagctaaa tacctgtcaa ctaatggtcc attatcagca aataacttgt
     8581 atttaaaatc tgttactgtt ttagaaatat aatcacaaat ttgttgtaaa aagtccttta
     8641 ctacaatgtg ataatccttt ccatgacaat attttgataa atttgaattt ttattctttt
     8701 cttgtctatt tatatgatat ggaaatgcac agactattat agagttagca tccttcatta
     8761 tatgtcttgg gttaattctg ttctcaatta ttggttcttc cataccagtt aaataacctt
     8821 tttgttgtct gtccttaagt atcttttcta aattatcata cttatctacc cctgctactc
     8881 ctacacagtt taatcctatt aaattacaaa atgcttttaa ttccttattg tccataaaat
     8941 tattcctttt taatctaatt gactttttaa atttatttaa tttttttagt ctttttaacc
     9001 ttttctagct actaactctt ttaaatactt aatcttttta gctattcaat tttcttacct
     9061 gcttaactct tttagatact tcgtcttttt atctatttaa tttttttacc tactaactct
     9121 tttaggtact tcgtcttttt acctatttaa ttttttacct actaactctt ttagatactt
     9181 catcttttta cctatttagt tttttaccta ctaactcttt tagatacttc atctttttta
     9241 tttactaact cttttagata cttcatcttt tttatttact aactctttta gatacttcgt
     9301 ctttttcatt tacttattta acttttttat ctacttaact cctttaaata attagtcttt
     9361 ttatctattt aattatttta cctactaact cttttgaata cttagtcttt tttatttatt
     9421 taattttttt atctactaac gcttttagat acttaatttt tttatctact aactccttta
     9481 tatacttagc ctttttcatt tacttattta acttttttac ctactaactc ttttagatac
     9541 ttagcctttt tcatttactt atttaacttt tttacctact taactctttt agatacttag
     9601 cctttttaat ctattcaacc ttttttaccc acttaacctt cttacctttt aatataaact
     9661 ctaaaacttc aaatatcatt agttatattt tgacattttt tctatagtta agcaaaaaga
     9721 ttgatttttt cttataatta atatcccatt tacattgcca tttattaagt ctttacttga
     9781 cttatatgga tttcttttta ctctctttac taaactatac ttattaatat tataaaggtc
     9841 ttataatagt ctaatatgaa tatgtccatc atatatcaaa ttttaaaatt tgtaagcaaa
     9901 atacttatct tatattacta catctaaagt ggaaaatttt acaaaaaagc tctgtaaaac
     9961 actttaaata acaatttgct tttaaatcat atacagatta taataaatac tgtataagga
    10021 tggtgaaaaa tttgattaaa ataacaattc caggtagacc tattagtaaa tctaatttta
    10081 agcttcataa cataaatggt caagcatgga tgccttcaaa gggaaaatat tctaagtatc
    10141 ttgcatacga aaacatgatt gcaggtttta taaaccaaca gtatcaaggg gaaactgttg
    10201 aagaaaatct aataacagtc ttgaaattat tcttcccaaa taagagaatg ggggatttac
    10261 ataattatcc aaaaagtatt tgtgatggta ttgaaaaaag tggaataata aaaaatgata
    10321 aacaacttaa gccagtactt ttatttgatt ttatagataa agataaccct agggttgaaa
    10381 ttgaacttta tccagtatct aaatacgata tatcctataa tatttttgaa aaataaagat
    10441 tttatgtgtt attcgacatt atattgatac tccagtattt gaaactatac taatacaaca
    10501 ataataattg tgttatgtct atactcaaac aaatgttttt aaaaagcatt aagtaaatat
    10561 ttaataagta ttaaacaaat attaaattta tataatagct ataactatgt aaatttaata
    10621 tttgtatata agaccacatc taataaaaag aaatcggagt aaatttaata caccgatttc
    10681 aataaataaa atctatccta gcttttcaac aacagtctta agcatttctc ttataggaag
    10741 ttcatatgga catctactct cgcaaagccc acagtcaata cactcagtag gtttaacatc
    10801 cataacctca tatttttctt tagcccactc ttttaatcca tatctagtat aataaccttc
    10861 acataaaaaa ctcaatggta tatttattcc tacagcacaa ggcatacaat attcgcatct
    10921 tctacagaat tttttaccta aagaatttct tatttcttca atttccttat tatctttttc
    10981 atctagcaca agattttcaa gtacagcgac attttgtcta acctgttcaa cactctccat
    11041 acctggtata actacatcta tgtactcttt agatagtata tatttaatag ccagtgttgc
    11101 attgtctaaa gcacctccag ctaaaggttt cattactatt atcccaattc ctttttcatg
    11161 agctttttta aacacttcat ctgcttgtcc ttcaactata ttgtaaggga attgtatagt
    11221 gtcaaatttt ccatcttcta tagctttttc tatagtattt aaattgtgac tagttatacc
    11281 aatatgtttt atcttacctt gctcctttgc ctctaaaagg gcactataag ccattttatc
    11341 tttaaaaaga ttatcatact cctcttcttt aacattatgg aattgatata aatctataaa
    11401 atcagtttta aaattattta aacttatttc aacatctctc ttcattgaat cataatctct
    11461 ggacatactt ttagtagcta agaaaaattt atctcttttt ccttcaatgg caattccgat
    11521 tgcttcctca cttattgtat accctcttgc actgtcaata aagtttattc cttgtttttc
    11581 taactcattt ataactaagt tagtatcatc ttgagtaatc ctttgtattg gaatgccccc
    11641 aaagccaact ctctttattt tcatgttagt attgcctaaa tttctcatat ctttccccac
    11701 ctaactttta ataatttttt atataatata aatatgtcat ctttaattat acaacaaatt
    11761 atgagaatct ttgcaatata tttacatact attcataata taattatatt ttatatcagt
    11821 ctattcataa tataatcata ctttataccc agttaatttg tatacaattt ttgttattcc
    11881 tatccattaa tttaaattat cacatttcat gattatatct ccaccctaat tatgttgagg
    11941 gcggagtatt tcacatattt ttaataaagg tttatttact agatagtatt tcttttatca
    12001 ttttaagctc tctcttgtga ccttcatcat caaaaggagt ttctaagaaa aatggtaaat
    12061 gttttagact aggatgttcc ataaaatcaa ttaatgcttt taatcctatc tttccttctc
    12121 ctataggagc atgtctgtct tttttgtctc caaatggcat catactatca tttagatgga
    12181 cagttttcaa tctttctatg cctataacct tatcaaattc ttccaaaacc ccatctaaat
    12241 cattcactat atcataacca gctgaaaaaa tatgacatgt atcaagacac acacctattt
    12301 tttctttatg ctcaattttg tctattatac tttttaattg ctcaaattta aaacctattt
    12361 cagttccctt tcccgacata gtttcaagaa gtatagttat attttcatca ccctttatgg
    12421 cattgttcaa tccttttact attctatcta taccaaaatc tacaccacca cctacatgac
    12481 ttccaggatg aaaacacatg tattcaattc ctaatgagtc catccttctt atatcctcat
    12541 ctatcaccct tgttgcaaat tcgtatacat catcctttgt accacccaga ttcatagtat
    12601 atggtgcatg tgcaagcagt ggtgcaaaat tattttcttt tctaatttct tgaaatttaa
    12661 ttatatcttt ttcatcaaat tctttagcat ttcctcctct tggatttcta ctaaaaaatt
    12721 gaaatgtatt tgcaccaata tatacagctg tctcagccgc attagcaaat ccttttgcta
    12781 ttgaaagatg tgttcctaat ttaatcatat taatctcctt ttatttgtac tacgacttgt
    12841 actcaatttt aattttgaac tactccacct taactacatt tagccaacat atgaagatgg
    12901 agattcctat ttgatagtac tttttcacta aaaattcata agctctaagg gatgttctat
    12961 cccaactaat ctcattcctc ttttaacata tttctagcat tgatttaatt tatattaatt
    13021 tttaatttta atatttacac tttaattata cccattttaa tcaatttcaa atattataat
    13081 cttacatact ttgtatatta tctaagcact tcttagatag tgtatacatc ttacactata
    13141 cacattattt acaaatacaa taggtataag aaaacaaaaa aatagagatt aaaaatcatt
    13201 gttttaccca taccaaaaac tattttttga ttattaagta aattttgatg ttcaatctct
    13261 aatattatat ctttaattaa tccataatta attttctaac taggacattc ctcctcaaga
    13321 gtaggaacta taaccatagc tatatcatac ttagaacctt caacattgtg ttgtctttta
    13381 tttaaaattt catctatctt tgacttcata gtgtcactaa gttcaactaa ttcatcatga
    13441 gtaagagaaa tatcatgata atcaactata ttttcatttt ctaaaacatc ttcctctgct
    13501 gccttataaa aaacttcact catattttca aattttgata tgtacaattc ttctttttct
    13561 tccccattgt ctaaagaaaa attaagtatc ctattcccta taaccacatt ttttgcactt
    13621 ggataatagt acttttccac aatccccgac tttatttttt cctcaactaa ttctaaaata
    13681 ccaactttgt ataacatttt tatatggtag tttatttttg catgaggttc ttccaataat
    13741 tgagataatt gttttgctga caatggcgat ttgtcaaatg cttttagaat atctattcta
    13801 cgtggatgcg caatagcctt tatgcactcc aaatctcgta ataccaatac ctctttcatt
    13861 gacccctcct aaaaaattta tttaattcat atttctttac aatttaatta tatattaaat
    13921 tatatgttgt tgtcaaaaaa atcctttctt aatattttaa aaaatattta atttgagttt
    13981 atttgacaac tcctctaaaa ctttaactcc tgcgacgctg ttaccttttt tgtcaagcgc
    14041 aggtccataa acaccaatcc ccatgcgtcc cggcacagaa gccattatac cacccccaac
    14101 tccagatttt gcaggaatgc caactttaat agcaaattcc ccagaagcat catacattcc
    14161 acaagtcatc ataaatgtct tgacaatcct agaaaccatt tcactcatta aatgctcacc
    14221 attttctata tctactccat aatttgcaag atttattcca attctcgcta aatctacaga
    14281 atctatctca attgagcatt gtttaaaata aaggtctaga acatcttcta cattccctct
    14341 gataaatcca tcacttttca ataagtaagc catagctctg tttctatctc cagtcatttt
    14401 ttcagactta tagacatcat agtttattcc tatattatca ttttttgcta actttctaaa
    14461 gaaactaagc attctttctt ctttttcttc taaacaactc ccatttataa gggaagtggt
    14521 tactatagcc cctgcattta tcattggatt acagggtttt tttgtgtcat taatttcaag
    14581 tttcattatt gaattaaatg ggtccccact tggctccatt ccaacgtttg aaaaaacgtc
    14641 ttcccaatca ttatccataa gtgccatagc aagcacaatt ggttttgaaa tactttgtat
    14701 agtaaacttt gtgctacagt ttccagcact ataaagatta ttttcgctat ctatgataca
    14761 aattcctaaa tcatttcttc tagcgttttt taattctggt atgtaactag cgacttgtcc
    14821 ataattagta tacttcttat tagaacttat aatttctttt aataatgtct catctaacgt
    14881 attcatatgc acctcttata ttatgttata tattctattt tagcacaagt ttatactata
    14941 ataaaacaat gttaaaaaaa attatacttt aaaattattt tctacaagaa tcaattcatt
    15001 tttcaaacaa tttaaatagt ttaaagagct taataattta gtaattcaat aatttaacaa
    15061 cctagcaatt tagtaatcta ataatccagt aatttaacaa cctaataatt tagcaatcta
    15121 ataattcaat aatttaacaa cctagtaatc taactatcta ataatccagt aacttaacaa
    15181 cctagtaatt tagtaattca acaatctaat aattcaataa tttaacaacc tagtaatcta
    15241 actatctaaa taatccagta acttaacaac ctagtaattt agtaattcaa caacctaata
    15301 attcaataat ttaacaacct agtaatctaa ctatctaaat aatccagtaa cttaacaacc
    15361 tagtaattta gtaattcaac aacctaataa ttcaataatt taacaaccta gtaatttagc
    15421 aatccagcaa tctaataatc cagtaattta aatttttata acaccaaaaa tttctaaccc
    15481 aacacctttt actcttttac tcttttactc ttttactctt ttactctttt aaaaaataat
    15541 cacataaact aacaaaatat taaacaaaaa agtaaaattt aaaataaaaa gcttgcttca
    15601 tacaaaagta taaaacaagc ctttttatat atcaactcaa actttaaaat ctgtatcaat
    15661 ttaattctcc actttacact taatatactt aacataaacc aatcacacta cttgctatgc
    15721 catttaatct tcataaaaca atctataata cacacattaa tcaactattt tacttcctcc
    15781 aataaattcc ctaagaattg aaatgcctgg tatgtcagaa gtatcgtcca attcaacaaa
    15841 atactgcaaa aactttagta atctagtagc ttcaatatgt gcaggattat cttcctttat
    15901 ttcttccaac aaaggtttcg caaatcgttt taaaactgcc aattcatata cacaagcaga
    15961 gttaaatata gcatctgcct cctcttgata cgggaatata ttcttctttt ctcctcttct
    16021 aactgatgcc cattgtaaaa tagtattatc agcactatat cctctaaaat tatagtctct
    16081 aacaattctt cttatcattc tcaaatcagc agtagggatt cgattatggt catccaaatt
    16141 tatttgagta agggcactta tgtatatctt aaatttatct tcatctggta tagatgatgt
    16201 taaaatagga tttagcccat gtataccttc taatataatt ggctgatttg aatctatctt
    16261 tatcttttta ccagtgtatt ctctcttacc ttctttaaaa ttgaatctta tatcatcaat
    16321 ttcttcacca gctaaaagtt tctttaagtc taagttaaac ttctctaaat ctattgcata
    16381 aattgattca aaatcataat ttccatattc atctaaagga gtatgttttc tatctataaa
    16441 ataatcatct aaagaaattg aaataggatt taaattattt actctaagat gtattgataa
    16501 tctgtgagca aaacttgttt ttccagatga agatggggct gctattaaaa ctactctctt
    16561 attgccatcc tttatcatat ccgctatttg ggataacttc ttttcatgca atgcttcaaa
    16621 tgtccttatt atatctccat attcattgtt ttctattatt ttattaagag atactacctt
    16681 gtcaattccc ataagtcttg accattcttc tgcttcataa tatacacttg atagcttagg
    16741 ttgaggtaca aatttcatag gaagagtctt atcttcttca ctaggtccta aaattataac
    16801 gcctccatta taaagcttaa tatcaaatga ttttatataa ccagttgatg gcaacatata
    16861 accataaaaa tggtctatat agtttctaca cttatatact ttagcactat catactcttt
    16921 gtatttcaaa agctctgcct tttcatacat ttcattattt tcaaagatag aaatagcttc
    16981 atttcttgta acttcaattt tttctataac ataatcgttg tttactatct ctttcatctt
    17041 gttttttata ttttcaacat ctgcttcttt aagctttcta tctatatgaa cttcacaata
    17101 tagcccatct gatagtgaat gttctattgt aactctgcta tcccaaaaga tttctctaca
    17161 tgccattaca aatataaatg acatgactct aaagtaaact ctctctccat cttcattagt
    17221 agtatctaaa aaatttatct cacaatcctt cttaactcta caatttagct cttttagttt
    17281 attgtctaca actgcaagtg ttatataacc cttatgctca tcacaaaatt cttttgctat
    17341 atctcccaat ctgattccag gggaattttc ttctaatgaa tattccttat cttttccttc
    17401 tatttttaag tttatttttt tcacttcaac atctccttcc ttttgatgaa tataaatttt
    17461 aattaatgag gcatatcaac aagataaaga gaggtattta accctctcct acaatattat
    17521 tcaatttact tatagtatct tcaatgtctt cgtaatttat ttctaatact aaatattctg
    17581 catctaatgt taagacctct ttaatcccct cttcagataa aattcctctt cctataccaa
    17641 catgtgtatc attgatacca tcattatcac ttaagtgtat cagtcttatt ttatccttta
    17701 agttctttac ataagcatcc tcatcaatta aacagtgtcc agtatcgtaa caaaaacaaa
    17761 tcttagaatt tttaatcctc ttaaatatat attcaaaatc ataagatata tttccaatat
    17821 ttgaaaaatc cccacctttt gaataagtat tctctataca tacatatgaa tttgtattta
    17881 atttatctaa aaaatccaca ctatttccta aatatttgtc tctattcttt ctaactctat
    17941 ttgtcataac atatcccaaa tgaaggttaa aataccttaa atcaaatcct tttagttcaa
    18001 attctatctt ctcaataaaa tttatccatg aatttcgtat atactctatg ttttcgcaag
    18061 tatttaattc cataggcaaa tgtatcccta tagataatcc taacttagaa attctctctt
    18121 tatatttaca taactcacta cattcagata tattgtcaat tccaatctct aaatgagtta
    18181 tttgctttat actctcacaa atgtttaaag cctcctcaat attaaacaat aaggacgcta
    18241 taccaactgc catacaaatc acctactcaa aaatacctat atccgtaaaa ggaaatactc
    18301 ttaaaataac ttttccttta atattttctt catctactaa tcccacttct ttatatctac
    18361 tgtctaaact aacttctcta ttgtctccca tcgcaaatac ttttccctta ggaatcacca
    18421 tatcaatatc gccttcagta taattatcat gtatatatga aacttcattt aataacttac
    18481 catttatata aaccttagaa tccttaattt ttagatggtc accaggaaca cctataaccc
    18541 tttttacaag ctcttttttc cttccattct cttgtaataa atcagtctta aatatcatta
    18601 tatctccaca ttttggttct gataacttat atgtcatcct gtttgctacc aaataatcat
    18661 gtgatttaag tgttgggtac attgattctc catttactaa tgttggtttt ataaaacgag
    18721 ttataataaa tgcaaaaaaa agtgcagtaa taattatttt tatccactct acaatctctt
    18781 ctttaatagt ttcattcatt cacaaattct cctttatagt tatattatct ttattgataa
    18841 tgtctcttaa acactatctt ttagataagt ctactaaata aggtgttgtc ttttttatat
    18901 agacaactat tgtgtatggt ctggtgattt cagctaatca gcgattgctc caaattttat
    18961 atgtttagtc aatagttcct atcttagaaa atggatacaa tctgactaga acctttccta
    19021 aaatggtatg ctcgtcgacc aatccaacct catcgtatct actatctaaa cttttttctc
    19081 tattatctcc cattgcaaat aattttcctt ctggaactac gatatcaata tctccatcag
    19141 tacgattatt atgtatatag gaagtctcat ctaacaactt atcatttaca tataccttgg
    19201 agtcttgtat ttttacatgg tcaccaggaa ccccgataac tctttttact aaatcttttt
    19261 tctttccatt ttcctctgtt aagtcggttt tgaatactat tatatctttg tattttggct
    19321 ctcctacctt gtacgcaatt ctgttaacta tcaaataatc acgttcaact aatgtaggat
    19381 acattgattc tcctttgact attgttggca ctataaaacg agttattgca aatgccaaaa
    19441 caagagctat gacaatcact tttatccatt ctacaacttc ttttttaact gcttcaccca
    19501 ctaaaatcgt ctcctctgtg ttaaatatta agtcaatcta cagcaaaata atctaccata
    19561 aacctacaat ataaatatta tacttctttt ttctaatagt ttaaaatact tttctaagac
    19621 ctttaggata atgattctta attacaccag aagattcttt tccccaacct aaaggtattc
    19681 cttcaaccga aaccaaaacc catcctctac tatcattaga atttacaaca tcccctttta
    19741 aatatttaag aattgattca ctctgtacac tgaagttttc aacatttttt acatcttcaa
    19801 cttttaagta atgagatagt gcatgagaag gctcaaatct attcttcttt aaaaccccta
    19861 aatgcaaacc atatctcaat acttttagtt ttttcgtatc tggatatatt tcaggaagta
    19921 ggtacaaatt ttctcctctt aaataaaatt tatttttttt attactataa aaatcaacct
    19981 ttaaaaattt cttttcaaac tccctataat cctttatttc tttatctaaa ttttttattt
    20041 ttaattcttt gacattacat tcttcttctt ctaatttttt tattttggca acaaaatgac
    20101 cttctccctt tatcttatga ggccataatc tttccatttc aataagttct gcatgtggat
    20161 attcactgat aaactcattt ataacatgct cattttcctc ccttgaaaaa gtacaagttg
    20221 agtaaattaa aatgccatcc ttctttaaca ttttatatcc atctcttata atctcttttt
    20281 ggatggattg acattctact acctttgcat aactccaatc tgatatggca acctcatcct
    20341 tcctaaacat accttgccca gaacaaggtg catctataat aaccttatca aaatacccat
    20401 taaacgtctt tcttaagcta ttagaatcgg tgtttgttat aatacaattt ctcgctccaa
    20461 atctctctaa attttcccct agagccttaa ttctactagc attaatttca ttagaaacca
    20521 ataaacctgt atcgtttagc ttagatagta tatatgtaga ttttccacca ggagcagcac
    20581 acatatcgag aactttttca ccctctttaa tatctgcctt tggtattaca ctcattgcag
    20641 aaggctcttg caaatagtat gctccagcct catgcagtgg atttctacca ggtctgtcaa
    20701 ttttatcatc ataataaaaa ccttcttctg cccatggtat ttgattcaaa tcaaacaact
    20761 ccaaatcaaa taattttttc ttgtcaattt ttaaagtatt tattctaagc ccagtagtct
    20821 tactttcctc ataacttttt ataaaatcat catattcatc ttttagtatt tcttcaatat
    20881 cttctaaaaa ttcctttggt aattctgtca aatctataat cccccttcaa tctgctttac
    20941 ttgtgtgtca tttattatac tttaattatt tatgtctttc ctttaaattt tatttcttct
    21001 tctttttcct aagattaccg aaaaacactt ttttagagtg attcatatat ttctcaagtt
    21061 tatcaagatt tatcttccac aaaattatta cataaactat aagtaatata atagcccatc
    21121 tatattctat catcaatatg aaatcaaaaa ctccatgtaa taaaattgga attaacactg
    21181 ccataaacaa atactcacgt cttttatttt tatttccctc aaatttatac ttagaaatat
    21241 agtagcccat agtaattgca aacattacat gagctggtat agagataact gccctattaa
    21301 taccaacttg cagtgctaga tttctacttt cagaaaaaat ataaaccata ttttcaattg
    21361 ttgcaaatcc taaagctaaa aatacagagt aaataatacc atctaacttt tcagtaaagt
    21421 gtttttcttt aattagggca ggaatcagta tcagagcttt tacaccttct tctacaagtc
    21481 ctgcaactac aaatgataca tatattagtt ctgaataccc ttcaaataca ttaaacttca
    21541 tcagtaaatt ttctaaaagt ataattataa cacttagaaa agcacctatt aaaaaaaatt
    21601 taattagatg gattaggggt tctctacaat atctatcttt agaccttatc caaaacatcc
    21661 ctatcaatat aggaactatt gctaataaaa ataaatctag cttcatagat tattttcctt
    21721 tcaaaatttt tgatttattt atgatttata tcatctactt atttttaact tattcgtgtt
    21781 ttaaatacat actaaaatat acattaaatt attttaaatt atttaaatac aaatatactc
    21841 aaatactcaa aaaaatttaa atttatatct atattattta acaaatttca agctatattc
    21901 atatcttgaa atataaggga actaaattca tttaaagaaa gggtgatttg atgcaagatg
    21961 gtttcttaac agtaagcata attgatgcta ctaacaatag acctattcaa aatgcagtag
    22021 taaacatata ttcaatgtct aatggtagcc aaagctcatc aactttatac caaaacctaa
    22081 gaagtaatga atctggtcaa gtgactggtt tagtattacc agcaccagat gtagattact
    22141 ctctacaacc atctgatgtt agaccttata gccagtatat agtggaagcc atagcagatg
    22201 gatatgagac agttgtcata gaaggaacac agttgttagc tacaattgaa gcaagacaag
    22261 gagttcccat gtctcctaga acaagaagta agcgttcttt ctcaagacaa agtgagttga
    22321 tttttgacat aggagaacat actctatatg gtacttatcc tcctaagatt ccagaaagta
    22381 acttaaaacc attaccacct cctactggtt tcgttgtatt agataaccct gtagtgcctg
    22441 aatttatagt agttcatgat ggtttgccag aagattcatc agctccaaac tattggattc
    22501 catttaaaga gtacattaaa aacatagcgt catctgaaat atattcaact tggcctgaac
    22561 agacaatcta tgcaaatgta atagctataa tatcatttac tttaaataga gtctttactg
    22621 aatggtatag aaataaagga tataacttta caataacttc aacaacagca tacgaccata
    22681 agtttataaa taatagaaac ttatttgaac caattaacgt agttgtagat gctattttta
    22741 atacttttat aaaaagacct cctacatcaa gacagccact attagcacaa tattgtgatg
    22801 gacaaaaatc ccaatgtcct gaccaaatga ctcaatgggg cagtaaagac ttaggtgacc
    22861 aaggatatga ctatgagagt atactaagat atttctatgg agatgagata gtatttgaaa
    22921 gagcacctat agtaagtggt gtaccagtgt cattccctgg tactacatta caagttggtt
    22981 cttctgggca gtatgttaga accatacaaa atcagttaaa tgctatatct aattcttatc
    23041 cagctgtacc aaaagttata gaagatggta tctatggaac agatacagaa aacgctgtta
    23101 agatattcca aggaatcttt ggtttacctc aaagcggagt agtagatttt aaaacatggt
    23161 atgaaatatc cagagtttat gtagctacaa ctagaatagc ttctttaaat cctttaattt
    23221 aaagcttgat ttagaacata aactcaaatt attgaataaa attattaatt taaaataaaa
    23281 atggctgtta tgaactaata tataccataa gtattactta taagtattgt agtcttaaca
    23341 gccatttttt agttataact tgttttttta gaatcttaaa aacagtgctt agcaacttag
    23401 atttctttaa aatattatat ttatttttaa gtttaataaa atgctattct aacgaaaaac
    23461 ctacttatta tttaaaatct cacttattat ttaaaatgat acttctcatt tagagtatta
    23521 ctccttatta tttaaaacac tatctatcac ttagagtatt acttcttatt atttaaaaca
    23581 ctatccatca cttagaatat tacttcttat ataaattatt atttattata aatacaaata
    23641 atattaattt aaaaagttat tcctaattta aataatacta ttatcttcat cagtgaacct
    23701 aaactgcaac tttagactta ttagctaaag ccaatattag acctaaagct atagatgcaa
    23761 atgcagaagc aattactcta tactcagcag ggaataaaaa tacaattgta tgacatggta
    23821 tccagaaaaa tggtatagtt tttaaaacac taaaaccaag gtaagcattc cagtccacaa
    23881 tatttaccac atctttaagt tttaccttaa tcccctttcc aacctcatat tttttatcta
    23941 taaaacagtc cataatctta tggaataaaa atacagttgg tgacattagt atattcatag
    24001 ttgcactctt aaatagagca tttgcaaaag ctattccctc aaatggaagc ataccatcca
    24061 cttgtgcttg acctacacct gatgagtaga ttgacattcc atatgtggta actacaccta
    24121 ttattcccca aactattgct ctataaaata ctcctactgg tattttccaa tccttataca
    24181 aaattctagc accaagtaaa tctcccattg tagctaatac agcaaacttt aaaaaacctc
    24241 caatatatgg atgtgcacct gttgcttcca taaatacttc tctactactt ggtattgcta
    24301 agacagcaat aatacaaata agaactccac tccatataaa atcgcctttt ttcatattta
    24361 taaatactat ttaatatata aatagttctc ctttctatta aagatttatt taatttacaa
    24421 tctatctaca attatttatt aatcacttat tattagatat tcattttcca tatttaaatt
    24481 tattctatcc ctttatctat ttattttctt actactctat ctaccattat ctatatattt
    24541 attttacgat tactctttca ccctaaccct ttaattatct atttttttct gatattctta
    24601 tctaccatca tccatatatt tattttacga ttactctttc accctaaccc tttaattatc
    24661 tatttttttc tgatattctt atctaccatc atccatatat ttattttacg attactcttt
    24721 caccctaacc ctttaattat ctattttttc tgatattctt atctaccatt atctatatat
    24781 ttattttacg attactcttt caccctaacc ctttaactat ctattttttc ttactattct
    24841 tatccacatc atccatatat ttattttacg attactcttt cactctaacc ctttaactat
    24901 ctattttttc tgatattctt atccaccatc atccatatat ttattttaca attactcttt
    24961 taccctaacc ctttaactat ctattttttt ctggtattct tatctactat tatctgtata
    25021 tttattttat gattactctt ttaacctacc ccctatactt tatagtaata tgtttttaac
    25081 ctttttcatt attcccaata tgctttataa ctacaaatac tctcccccct taaaaataat
    25141 aataaaatta atcaataaat ttataatcat attattactc tatttattaa gcaattacta
    25201 tgccaataaa ctttgatttt tttaaacaaa tcttaaaaat cctaaaatgc taattttctt
    25261 taacttttaa tgaatctaat agataacatt taattttcgt tatatttttc agaatatttc
    25321 tattattatt tttaagaaaa aaaaataatg ttaacatttc ttaacataca ttaaaaaatg
    25381 ttaacattat aaattgggtt taatactttt actttgactc gatttacttt tattctactt
    25441 cttactttat ttttaactta gactttattt ttttattact tatttgagtc ttatttattt
    25501 tattctcact ccatatgttc aattacattt gtctaaattc tttgatactt ttattacata
    25561 attttaacaa gcttaagtgt tatttgggta gtgcagttga taattattta aataatcatc
    25621 aactgcatgt ttatatcatt acagtagctt aatgtattta atcatactta cttaggattt
    25681 tgaaatatct tagagattat atttaacatt agtcaattgc tacataattg taagcaattg
    25741 aaatttgaga cccatcttca ttttctatag tgataggctt cataattttt aatccttcag
    25801 tttttatctt ttctaattct tgttccattt tcttaattgt ttcctcaagg tctttttgac
    25861 ttacatcttt gactgcatta gggtctttag catagtcttt taattcttga atatgtttag
    25921 catactcttc ataagtataa tgctcaacac catacttttc caattcagta gggtcagtta
    25981 ttcctgattt tattagagat tcaaatttag gtaaagcaat attagatgaa tcatttccat
    26041 ttgcaaatac tgctccagtt cctcctagta ccattgcaga taccatcatt gcacttataa
    26101 gggatttttt tattttcata accaatcttc ctttcttaga gatttatttg ggtagtgtag
    26161 tttaaaatga ctattgcaat agttatcaac cacatcctta gactatcaca gtagtcatgt
    26221 ttatgtcaag cctttttaaa aaacttttca ataaaaaata ctatctcaaa acaaaattaa
    26281 ttattttgag atagtatttt aattctaaat cttttatatt actaagattt atggttactc
    26341 tatccatgtt ctatattgtt ctttattaac tgctaacaaa ttcccttttc ttgatattac
    26401 aaagcgatta tcattatagc cacttcgctc aattgaaact tctcctgaat cagtataaag
    26461 tacatcctta ctattactag atttatttac atacgtttct tcactattct cagaattagt
    26521 tactttgctc atagatgatt cttcatgctc tctaaattca attacagata cttttgccgt
    26581 actaaaatga gtctctccat cacttttagc tttactaaca agaatatcag attctacttt
    26641 gggagaatat aaatcagttg atttactact tattgaaaat ccaattttat cactttgttc
    26701 attttttgtt aaaccaaaag attcaacctc attaatctct ttattttctg ttgcaaaaac
    26761 aagacctgtc ccacaaacta atacagttga aactaaaaaa gtaacataag ttgatttttt
    26821 aaatttcata atattaacta ttctcttttc tatttttgtc tttccaaatc cactacatat
    26881 tgtcattgaa aatggattat tctcctctgc tagacttatt agtgtggttg cataatcagc
    26941 cctatattct tcaccaagta tactcataac cttttcatca caactaaatt ctatgtcttt
    27001 atttagtaaa aaaaacatca cccaaactaa gggattaaac caatggacaa caagagctac
    27061 aagcataaat aactttaaca gattatcaaa gctattaata tgaaccattt catgagttaa
    27121 aacaaagtct aaagtttgat tattagcata attcatttgc tttggcaaaa taattcgtgg
    27181 ttttaaaata ccagatgtaa gtggtgtcct agtaaattca gatatgcaaa caataaatct
    27241 tctaaaacgg ctattcttac acatccattt tttaatgaac tcatgatttt ttatatgaat
    27301 tgaagtttta aaaagattaa ttattttaaa atatctaaag cataaaagta tgctaaacat
    27361 aatagtaact ataaaagaca aaatgcccaa catttccaac atcataatat tttcaatact
    27421 agacattatt tcaaattcgt cagaattgtt tttacctgta gagtcactag ctttcttaag
    27481 agtataattt tctgttgatg tattacccaa ttccacatca aaagtcgatt caattatact
    27541 ataaatacta aaaccagaat ttaaacttac tggtattaga agtttaaata aaacaatcaa
    27601 ccacaataat acaaatacct ttttaggtaa ttttttaaat aaacatgctc ttataaaaat
    27661 aactattaaa agtaatactc cagaagtaaa actcatttct aacaatttaa tgaacataaa
    27721 tcttacctca ctttaatttt tcaacaattt gtttaagttt tgtaatctca tcacttgaaa
    27781 gattttcttt attaataaaa gctgaaagaa atttttctga agaccctcca aacattttat
    27841 taattaaagt ttctgtttcc tgtttttgta cttcctcttt gcttattaaa gctttacaaa
    27901 caaaattcgg ctcaattctc tctattgcac cttttgacac taattttttt ataacggtat
    27961 acgtagtgtt tctattccat cctgtttctt cttttaaaat ttttactaac tctccagcct
    28021 tgctttctcc tctctcccat agaacttcca ttactttaag ttctgaatca aataatttta
    28081 tctccatata aaatcgctcc tcaaatatag ttatcacaat agtcattact ataataaaaa
    28141 actatcat
//
LOCUS       DAGSHT010000031        26833 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGSHT010000031 DAGSHT010000000
VERSION     DAGSHT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.032"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            150..3248
                     /locus_tag="MJB72_001228"
     CDS             150..3248
                     /locus_tag="MJB72_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY8262353.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3371..4657
                     /locus_tag="MJB72_001229"
     CDS             3371..4657
                     /locus_tag="MJB72_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY8262354.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5010..6116)
                     /locus_tag="MJB72_001230"
     CDS             complement(5010..6116)
                     /locus_tag="MJB72_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY8262355.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6151..7692)
                     /locus_tag="MJB72_001231"
     CDS             complement(6151..7692)
                     /locus_tag="MJB72_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY8262356.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8054..8800)
                     /locus_tag="MJB72_001232"
     CDS             complement(8054..8800)
                     /locus_tag="MJB72_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8262357.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKHIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9012..10322)
                     /locus_tag="MJB72_001233"
     CDS             complement(9012..10322)
                     /locus_tag="MJB72_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8262358.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10335..10805)
                     /locus_tag="MJB72_001234"
     CDS             complement(10335..10805)
                     /locus_tag="MJB72_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262359.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10809..11660)
                     /locus_tag="MJB72_001235"
     CDS             complement(10809..11660)
                     /locus_tag="MJB72_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBY8262360.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            12496..13407
                     /locus_tag="MJB72_001236"
     CDS             12496..13407
                     /locus_tag="MJB72_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBY8262361.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            13582..14079
                     /locus_tag="MJB72_001237"
     CDS             13582..14079
                     /locus_tag="MJB72_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBY8262362.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAINDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            14823..15185
                     /locus_tag="MJB72_001238"
     CDS             14823..15185
                     /locus_tag="MJB72_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY8262363.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            15250..15867
                     /locus_tag="MJB72_001239"
     CDS             15250..15867
                     /locus_tag="MJB72_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBY8262364.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            15864..16349
                     /locus_tag="MJB72_001240"
     CDS             15864..16349
                     /locus_tag="MJB72_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBY8262365.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            16392..16910
                     /locus_tag="MJB72_001241"
     CDS             16392..16910
                     /locus_tag="MJB72_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBY8262366.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            17046..18005
                     /locus_tag="MJB72_001242"
     CDS             17046..18005
                     /locus_tag="MJB72_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8262367.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSEISISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            18031..19734
                     /locus_tag="MJB72_001243"
     CDS             18031..19734
                     /locus_tag="MJB72_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBY8262368.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            19987..22089
                     /locus_tag="MJB72_001244"
     CDS             19987..22089
                     /locus_tag="MJB72_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBY8262369.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKNNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            22102..22542
                     /locus_tag="MJB72_001245"
     CDS             22102..22542
                     /locus_tag="MJB72_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBY8262370.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            22578..23381
                     /locus_tag="MJB72_001246"
     CDS             22578..23381
                     /locus_tag="MJB72_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBY8262371.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            23421..24017
                     /locus_tag="MJB72_001247"
     CDS             23421..24017
                     /locus_tag="MJB72_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBY8262372.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            24123..24986
                     /locus_tag="MJB72_001248"
     CDS             24123..24986
                     /locus_tag="MJB72_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBY8262373.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            25053..26042
                     /locus_tag="MJB72_001249"
     CDS             25053..26042
                     /locus_tag="MJB72_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBY8262374.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            26134..26745
                     /locus_tag="MJB72_001250"
     CDS             26134..26745
                     /locus_tag="MJB72_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY8262375.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT        10493 a         3044 c         4559 g         8737 t
ORIGIN      
        1 aataataatt ctatttacta aaaaatagaa cctcattaag gtactatttt aaaacataaa
       61 gaagtataat atcattgcca aatatatagc aaaattttaa aagctttaaa aattcaatct
      121 ggaaaatgaa taaaaggaga tatatataaa tggatgtaat aaatttaata gatgagttgg
      181 atgaaattgt atatgtttct tctttagata catatgaact tttatatatt aatgaaattg
      241 ggaaaaaggc aatgggactt aatgatatat cccatttaaa atgttataaa gcattacagg
      301 ggaaagattc tccctgcgaa ttttgtacaa actctatttt aaaagaagat gaatattaca
      361 tatgggaaaa tacgaatact cgaacaaata gacattttat attaaaggac aagttaatta
      421 aatgggaggg caaacatgca cgattagaaa tagctcttga tattacagaa aaggaagata
      481 taagtaagtc aatagcagaa aaattagaga taaaggagtt attagttgga tgtattaaac
      541 atcttataac agaatctaat ttttctgatg ctgtaaatct tgtattaacc aatattggta
      601 aatttcataa agctgataga gcatatgttt ttgaagtatc agaagataga acaaaagtaa
      661 ctaatacata tgaatggtgc aatgaaggtg ttgatgaaca aaaacaggca ctaacaaatc
      721 ttgatattaa tgaattaaag aattggtttt tattattcga acaaaaaggt tttgtaataa
      781 ttgatgatat agaaaatata aaagaagaac agccaataga atatgaaatt ttaaaacctc
      841 aaaaaattga aagtttaatg acatcttcac taaaacaaga tgataaagta gtagggttta
      901 ttggagttga taatccacat aaagctgttg gagatacctc tcttttgaca tccctatctt
      961 attttttatt aactgagatg aaaaaaagaa aagtagaaaa taaattaaat tatatgagtt
     1021 actatgactt cttgactggt gcatataata gaaataaata tattaagtat atagataatt
     1081 ttcaaaaagg aaaagttaaa tccataggag ttgtatttgt agatataaat gggttaaaag
     1141 atattaatga taactttgga catgctaatg gcgactctgc tattgtgtca gcatgtgtaa
     1201 taataaaaaa atactttcat tcttataatg tttaccgtat tggaggagat gagtttgttg
     1261 tattatgtga agatatatca aaacaactat ttgaagaatt agtgagttgg ttaagtgaag
     1321 aatttaagga tgagatgata tatagtgtag cagtgggcag tatttggagt gacgataata
     1381 ttgatataca taagctaatt aaagttgctg atgagagaat gtaccaagat aagaagaggt
     1441 attataataa gaacaaactt agaaataatg aaatgaaagt aattagtgac ttagctgatt
     1501 cgacgaataa tattttattt aaacataaag agttagttga taaggtttat tttgatgatt
     1561 taatgataaa agggtattct aaggcttatg aaaaattagc ttataataca tatgatataa
     1621 ttttatctat ttgcattgat acgaatttaa ttactccaat ttatcaatca gagagcaata
     1681 tttttagttt tcctataaag gaaaatcttg aaaaaacttt ttcatacata aaaaaatatt
     1741 taattcatcc tgatgatata atattattta atgataacca ccttctaaaa tctcttgata
     1801 gtcttaataa tagcaagcaa gacgatttag aactaaattt tgagtataga cgtttaggaa
     1861 tagataaaaa gtactattgg gcattaattt atatgttaaa gattagtagt aaaaataagt
     1921 taggtgagaa acaaacacat attttagttg ctataaaaaa tatagattat cttatgcaac
     1981 tacataaaaa ccaaaaagat acattaacag gactatacaa ttatgaaagt ttttgtgaac
     2041 aaataagttt aatgcttaaa agatatcctg acaagcgtta tgcaattata atgatggata
     2101 tagataagtt caaggtaatt aatgacattt atggagttag aggaggagat aaagctttaa
     2161 tatatgttgc agagttgatt caaaaaaaca ttggttctaa tggtatatgt tgtcgtatgt
     2221 atgcagatgt attttgcatt ttttatgaga gttactctga tagagatatt cataaggtaa
     2281 tttataagat taaaaatagc ttaaatagaa aaaaatttga atatatgtta gtaccatgtt
     2341 ttggggttta tagaattttg gataatcata ttccaattac aaatgcgtgt gaaatggcag
     2401 gatatgctca caaaaaagta aagaaacaaa gtatcaataa ctatatattt tatgatgata
     2461 ctataagaaa tgatgcactt gaagaaaaac agatggaaag ggaaatggaa tacgcacttg
     2521 aaaatgggca attccatgta tatttacaac ctaagtataa tcttaatgca aataaaattg
     2581 ttggtgcaga agcattggtt agatgggttc atccagaaaa aggtataata cctccaatta
     2641 aatttatttc actatttgag aagaatgggt ttattatcag attagatatg tatgtatggg
     2701 aacaagtatg ttctttgatt agaaaatgga tagatttagg tgaagagcca gttcctatct
     2761 ctgtaaatgt atcaagatta catttgcata atccacattt taaagaaatt gttttaagct
     2821 taataaataa atatagtata ccaaaatctt ttttagaatt agagcttaca gaaagtttat
     2881 ttattaaaaa tataaaatta tttactaaga taatagccga ccttagaatg gatggtattg
     2941 ttttaaatat ggatgacttt ggttcagcat attcttcatt aaatatgtta aaaaatatta
     3001 atattgatac attaaagata gattgtggtt tctttaatga ggaaggaatc acagacagag
     3061 aaaaaatagt tttaaagcac actatagcca tggcaaaaga tttagatatg gatgtagtgg
     3121 cagaaggtgt agagacaaaa gaacaagctg aatttttagt gactttagat tgtgtggtaa
     3181 ttcaaggtta ttattattgt aagcctattc caagtgctga ttttgagaag ttactttatc
     3241 aaaaataagc taattattcc attctaaatg taggtaggat ggagtagttc aaaataatta
     3301 aatagaagta gaaaatagtg aggatataat ttttaaaaag tataatattt atggattagg
     3361 agcgaagttt atgatagtgg gtattatagg tccttcagac tctgggttta agataaagga
     3421 tgatttaaaa cagatagaca gtgatttaaa gactaaaata tatgtaagag aaaaagtagt
     3481 agatacaata gaagttattt ctaaatgtga agacgaatgt gatgctatta tatttacagg
     3541 atgtgcagtt tatgaattta taaaatctaa gtatgagata agtaagccac attcatttgt
     3601 accgagaagt gggacgagta taatgaaggc attttgggca ataaaaagtg caaatataaa
     3661 attagataaa ttcagtatag atgtagttga tagatatgtt gtggaagatg cacttaaaga
     3721 atttgatatt aaagcaacga gtgtattttg taatccattt tctttggaag taggagagtc
     3781 tgaacttgta gaatggcata taaaattatt tgaagaaaat aagacggata taatgttgac
     3841 tggttttggt gcagtatata atgaattaaa agaaagaggc tatcctgtat ttcgtttaca
     3901 agctactata caattaatca aagaaagtta tgacaaggta aagagtgaat atgctctaag
     3961 taaggcaaga ttttcccaaa ttgctgttga aatattgagt ttaatagatt ataaagagaa
     4021 gatagataac tattattctg acatgataaa aaaatcagat atggataagc ttgtagtaaa
     4081 ctatgtaaga agcattcaag gttccttatt tttacttgga agaaatgatt atgttgtatt
     4141 tgttcataaa ggggtagctg ataatgagta taattatgat aagctattca atctaaagag
     4201 agagataaaa gacatgggct tttctcttag tataggaatt ggtactggag taactgcgta
     4261 tcaggctgaa aataatgcac ataaagcatt aagacactcc atagattcaa aagaaattgg
     4321 tatatatttg gtagatgaag atgaaaacat aagaggacct ttagcttccg aaaatgaatt
     4381 aaattattct ttgatgctat ctgatgaaaa ggtaatcgaa atatctaaaa agactggaat
     4441 gagttgtgag tcagttgcaa aaattattgc tataaatgag aatagaaaaa gtaagattta
     4501 tgattcaaaa gagttagcag agtatctaaa tgtaagtgaa agaagtgcaa gacgtatatt
     4561 aaataaaata gtaaatgcag gtttaggtcg aatatgtgca aaagagacta gtattggtgg
     4621 aggaagacca aaaaatatta ctgaaatact gttttaagat attttataaa actgtataaa
     4681 tcattatatt gtataaaatt ctacaacaaa ttactatata aagtattaaa gcttattatg
     4741 aaaaaaatta gaacaaatta ttatataaaa tactagagct tgttattaaa aatactagaa
     4801 taaattatta atataaaata ctagagccta ttattaaaaa tgctagaaca aattattatg
     4861 caaaatattg aagcttatta tgaaaaatgt tagaacaaat tactatataa aatactaaaa
     4921 cctatgtaaa acaactttaa tatataactt ataaagttga agatacatag gtttttattt
     4981 ttataattta aagagttcta ttatgatatt tataatttaa tcatatgata aactattttg
     5041 gcagtatcat ataattcgtc tatatttaca tattcattaa ctgtgtgtac atcatacata
     5101 cctagactta atatagcaca attatatcca agattagcta atatatttgc gtcacttcca
     5161 ccaccaatta ccattggatt tggaacaaca ccaacttgtc ttattgcttc ttcacttaac
     5221 ttgaaaacat gactatctct actaagttca aaactagggt aagacatact atgttcaaaa
     5281 gtataagtag tattaaattt agatgcagca tccttacaac atttttccat atgattaagc
     5341 tcatactcta aagtttcagg tatatgagac ctaatttcag cagtcaatgt aaccttatca
     5401 gttactatgt ttgtagctcc accaccttct attttaccta tatttgaagt agtcaatgaa
     5461 tctattctgc ctatgtgcat gttgcttatt gcatgagagg ctactaatat agcatttaac
     5521 cctttttcag gttctatacc agcatgtgct ttctttccat gaaaagagat ttttatagat
     5581 tgttgagcag gagctttgta tgctatcgaa ccaatagctc caccagaatc taaaatcacc
     5641 atgtctttac aaggcaagtt gtcaggatta aagtttttga caccatgcat accagcttcc
     5701 tcacagattg taaaacaaag ataaatatct ctatgtggta ttttttctgt aataacatgt
     5761 tctaaagctt caagtatagc agctattcca gctttatcat ctgcaccaag tgtagtagtt
     5821 ccatcacttc taactacatt accatctagt attggtttta cacctaaaca aggttgaact
     5881 tggtccatgt gtgcacataa acaaagtggt ctacttcctt cttcaccttt tatataggca
     5941 atgacattac ctgtattacc accatatttt tcacctgcat catctataat ggcatctata
     6001 tttctttctt ttaagtagtt taccaaccac ttagacattt ctaattcttg attagatggg
     6061 ctatcaattc taaccatatc cataaaatta tttataagtc tatttttatc taacataaat
     6121 tatttctcct tttagaaaga tttaaataaa ttacatgcta tatgaaggaa gtattccagg
     6181 tcctagtgga atattaaata ccataaacaa tattaatagt atagtccaca ctattaagaa
     6241 tgatatagag tatggtatca tattagcaat tacagtaccc ataccagcat ctttatcgta
     6301 ttttcttgtg aatgctagta taatagggaa gtacggaaat aatgggctaa gtggatttgt
     6361 tatacaatca cctatacgat aagccatctg agtaagtgct ggattgtatc caaccatcat
     6421 aaacattggt acaaatatag gtgcaagaat agcccacttt gcagatgcac tacctataaa
     6481 taaatttata aaacatgata atataataaa tcctatcaac aataaaggtc catttattcc
     6541 tgctgatttt aaaaattctc caccctttac agctagtatc agacctaaat tactatttgt
     6601 gaataattgc aaaaattgtg aagcagcaaa tgctagtatt atatatccac ccatatcact
     6661 cattgcacta gccattatag agactacatc cttatccttc tttattttac ctatagtttt
     6721 tccgtataca acaccaggta tgaaaaatgc tagtgctatt ataggtacca tacctttcat
     6781 aagaggagca ttgtttgata gtaaatctcc tgtttctggg tcagctaaga atggtttttt
     6841 accgattaca ctaagagcta ctataatagc cacatatatt aatatactta taccagcttt
     6901 tttaagccct ttcttttcta catcactaag acttccatca acatctagtt ctgcatttcc
     6961 ttcatattta ccaaatctag gagctacaat tttttctgtt acccatgttg ccaaaagtat
     7021 tagaacaaaa gttgatgcta tcataaaaaa taagttcatt gttgcattgg cttgaaatcc
     7081 agaatcaatc atttgagctg ctggaactgt aaaacttgca gcaagtatat cattgacact
     7141 tatcataatg tttgcggcaa atcctagaca gactgctgca tatccagaaa ataaacctat
     7201 aagtggatgt ctaccaacac caagatatac aagtgcagct aatggtggaa ataatattgt
     7261 acctgcatct gaagctatat tggcaagcat acctatagtc attacaacaa gagttactct
     7321 tgtttcagga atcttgctta ctgatgcttt gattgttgct gtcataagtc ctgttttgtc
     7381 acatacacca gcacctatca tagttactag aactagaccc aaaggagcaa acccttggaa
     7441 attgctgact atgtttccta aaagaatttg aagctgttct acagtcaata aatttacagc
     7501 ggttatagtt tctccagtac caggatgaac tactgatata cccatagaac caactagact
     7561 agatagtata agtaaaagta cacataaccc taaaaatatt gtaactgggt ctggtagttt
     7621 attaccaaca gtttctattc tgtttaatgt cctgtcaaaa aagtttttct tcttaactac
     7681 gttatcattc ataatatcct ccctaatgtt taaaaatata attaaaaaat aagtattgtg
     7741 gttaatctta caatataaat tctgcttgtc gatttaagac gtgtccacaa tatgtcctaa
     7801 aaaaagtata aaataaactt tttcctgtgt caatgcagtt ttctgaattt tctggttgag
     7861 tggtgttagt taaatatgct ttaagtagat taatgacgaa ttatatctaa tgtatttaaa
     7921 taacatatgc gctttaaaat taattttaaa acaaaatttt atgaaaagct agtggaagat
     7981 taagttatta aaataagtag cgtagtaaag tgttagattt tttaattaat caaagaaatt
     8041 tactttgtat tttctacaaa aacctgtgaa tatcttttga attatgtaga ggatataaat
     8101 ttattttatt tacatagatt agattttgtt ctaatttact gtattcaaat ggttttccat
     8161 gtccaggata aatcatttta ggttttgtat tgatgattgt tttccaagat tgaataaatt
     8221 ctagtttgct ttctgcaaat atggtaattt tgtgagtact tggaaatcca ttcattgcag
     8281 aatctccaca aaataaaaca ccatcatcaa ttagtagtga aattgaatca gcagtatgtc
     8341 ctggcgtatc tataattttt ccatttagtt ctttttcaat ttctttacga ttcttgtcag
     8401 acacaagaat acaccttttt tcaaattctt gtttaagtgg agaaaaacta tgctcacctt
     8461 ttccaaatag tttcataatt tggcaaaaga gaaaggcagt acggctggta caaccaccaa
     8521 taaaagagtt ttgaccttta tatagaattt ctaaagcttt atcatgcata acaatctttg
     8581 tattaggaga ttcgtctaga atctgattta gaaaaccaac atggtcatca tgagcatgag
     8641 ttagaaatat gtatcgaata tgtttgattt ttatgttgaa tttagaaatt cgttttttga
     8701 aatgtttaaa accattttca tatccagtat caatcaagac aaagccatct tgaatagggt
     8761 acaaccatgt atttataatt cgatttcctg cattaaacat tttttcacca ccttgaatta
     8821 gaatagaaat ttatattaat tagaaagaat atagcatagt tctactgata tttcaacgta
     8881 gctctaaaag ttttaaggag gataatatat tttagtttaa tctaaaaact aaaagaaaag
     8941 aagtatactg ttacaaaaac aaagagtggt ataactttta cagtagatac caccctatta
     9001 taaaaataga cttataaagt tttatcgtgt ttttctaaga atgttagata ttcttcttta
     9061 gtcataactg gagtaaaggt atctaatata tctttagcat ccttagcatt atcaaaaaga
     9121 aggtctacaa ctgtaagtgc cattgcttta gcaggaatta tgtaagctaa atcttcatca
     9181 actatttcaa aatctcttgt atgaagagct ccttttacac caccaatcat agggtgaaga
     9241 gtaggcatta tatgagacac atctccaaaa tcaaaagagc ctgtaaaatc tccaccatca
     9301 ataacttttc cttttagacc taaatcttca agattgtttt taaataaatt atcaagactt
     9361 ttgtatcgta atattggaag atatccagga atttcagtta tctcaacatc agctccaact
     9421 gccattccac cagccatcaa agccttattt attctttcat tagcatcaat cataccattt
     9481 atagttctag ctctaacata tgattccata tgaacatcag caggaacaac atttactata
     9541 tctccacctt ttgtgatgat agggtggaat ctaactcttt cagactcttt aaaagtttct
     9601 ctaagtgcat tcacgttgtt tatagcaagc attgctgcat ttaaagcatt tattccatca
     9661 tgtggagcag aaccagcatg agattcttta cctataaatt taaccttttt tccaacaaat
     9721 ccattactct caggaccaac tagagcctta tcttctccta aatcagaagc atgaaacatc
     9781 atagatatgt ctatatcatc aaaataacct ctttttatta attcttgttt tccaccaaaa
     9841 taagttattt ttccttcttt ttttaattgc tctctgtatt ccaactcaat aaactcttca
     9901 gcaggagtag ccataaaaga tactttacca tctaaatgtt ctaaaacgcc actttttact
     9961 aaaccaacag cagcacctaa cattccagct atttgaatat tatgtccaca agtatgagaa
    10021 gcacctattt cattagcatc tttatgttct ttacatgata taccatccaa ttctccaagt
    10081 atagatatat gaggaccttt cttgctagaa tttgcatctg ctttgcatcc tgtaactgct
    10141 atattttttt ctacagataa atttaattct tgttcaaaaa agtttgaaac agcttctgta
    10201 gttttaaatt ctttatatcc atattctgga tttttgtata ttccttgacc tatttttaat
    10261 attttttctc tattttcatc tatagttttt attactagag attttaattg ttctttattc
    10321 ataataaatt cctcctatat tttaccttga atacttaaaa ctatatgagc tatactcgca
    10381 catgcaaaga atatagttgt aaatacaata agtgaaatta taactatttt ccatgacatt
    10441 tcttttaaag cttctatttt gtttccaaca gaaattcctg caaaagctag tagtggagta
    10501 gttatagcca taaagttaat attattagtg ttagttaaga atacttcaga tgttggacta
    10561 tatggcatac taagtaaaag gccgattata gttgcaaatc caaaagctgg aaagattgat
    10621 ttaggaaata aatcttttat aatcatagct gccatagcag ctaatattag catcaccata
    10681 ccaggaagag ctgtaactat agatgtacca acgctaattc tttgtcctat aagaattata
    10741 actccaataa cagacataac caataattga tatgaacttt tctttaaatt taaattagca
    10801 aacatttatt agcactctcc ttctgtagat ttaatttctt tagttttttt atttctcgaa
    10861 aattttggtt ctaaaatctt atacataaaa ttagtaaatg gtaaggatac aaaaactaag
    10921 aatataattc ctgttatact agacatcata ttgcttgttg cagcataggc aagtattgta
    10981 tctgccattt caggtggtac catggtagat aatgaaccag cagctgctgt catcatactt
    11041 ccactaccaa caccacaggc cattcccaaa gcatatgggt gaagaccact ataaatagat
    11101 attgaaccta gtacactaaa gtatatagta ccaagaactg ttcccattaa atatgtacca
    11161 agtacaccac taccttctgg tgagtttatc ccatattttt ctgatataac tcctaaagaa
    11221 ggttcacgac ttatacttgc agtagctcca acagcttctc ttttcattcc aaacattaat
    11281 gcaagaggta atgcaacgat tggtgcaagt atatgaccaa attcttgagc gataaacgct
    11341 ggtccagctt gaattatttt gtcaatgttt ggtccaactg tagtaccata ttttatacca
    11401 agcattacta gtgcaattcc aacaacttca ccagcaatat ttatttcttt ttctccaacc
    11461 acttttttaa gtgctttaat ttttttacct aataaatctg gtgttattat aagtccaatt
    11521 ataactgcat aaagcatagg gaacagtact atagaggctt ttccaatagc tatagttctt
    11581 tgccctataa attcacatat tactaccaag attaagcaag ttaaaaatac ttgaataaaa
    11641 tgttttttat taatttccat gatttcctcc tcaaaagaat aaatatttta cttatctgaa
    11701 atgagaaaat acgttttctt aagtataata cgttttccta aataaaatga aaaaactatt
    11761 tcaaataagt ttcagaaatg tcaataacat gtccttaatt aaggataaaa caaaaattta
    11821 acattgtcaa cgaaaaatat ctaaaatatt acatattttg aatatattgt aatatagctt
    11881 tattatgaaa aaattgttta aatattgtag gaataatagc taaaaagctt taaaattagt
    11941 aatataaaat tgaaaaataa taatatagaa ttgagaaata gtaaattgaa attttaaaat
    12001 aaatgagtgg aagtagctta gtaaagtatg gaaatgaata ttacaataga aaataaataa
    12061 tatgtaaaga ctttaaatta attttaagta ttaagtacaa atactaattt ttgatataga
    12121 aattgaataa agtaaatgat aataagcaaa tgaattaaga tgataataag tgaattgata
    12181 tagaaataag taaattaagt aagacgataa caagtgagat aataataaat gaattgacat
    12241 agcaataagt aaattaagta agacgataac aaatgagatg ataataagta aattgatata
    12301 gaaataaata aattaagatg ataatatcca aagtgagaaa gataatagaa agtaaataaa
    12361 atcatgaaag ataatagaaa gtaaataaaa tcatgtaaga ataatttaga taagattcta
    12421 agattttatt tatttaaaaa ttagtatgat taatttagat taaataaatt taataatttt
    12481 agtaggtggt aatgaatgaa aataaataga ttaacagaaa taatagtaat tctattaaat
    12541 aaaaaattag taacagctaa agagttggca gataggtttg aagtttctac aagaaccata
    12601 tatagagata tagaaactct gtctatgtcg ggagtaccag tatatatgac gaaaggcaaa
    12661 ggaggaggga tatctttaat agaagaatat tctatagaca aggctatatt gtcaaaaaaa
    12721 gataaagaga gtttaatagt tgcactgaaa acattacagg cgactaagta tccagaaata
    12781 aattcagtag taaataaaat tggctcaata tttggagagc agaatttttc aaactggatt
    12841 gaaatagact ttactgaatg gggaagtaat tttaatgaag atgataaatt tacaaaaata
    12901 aaagaagcta ttttaaggcg taatactata aattttaatt atgtaaatag tttgtcaagc
    12961 cagacaaaca gaactgtcga accactgaag cttatgtata agagtaagac ttggtacttg
    13021 tatggatttt gtaaactaaa agatgatttt aggatattta gaattagtag gataaggaat
    13081 ttatctataa aggatgaagt attttcaaga aagataatag aagaagtatg tttgaatgat
    13141 tctaaagtaa ttaaggaaaa tactataacg ttgaaattga ggtttaaaga aaagatgctt
    13201 tttagagtat ttgatgattt taataaggat ttaataacta aaaatgaaga tgatacatat
    13261 gatgtaatta cagaatttcc tattggtgaa tggatttatg gatatatttt atcgtttggt
    13321 gacaatgttg aagtacttga acctaaagat gttagggata atgtaataac tagactaagg
    13381 gagttgtcta aaatttattc attataatat aatgctagat taaagatttt attaaatata
    13441 taatatcttg catttagtat ataatatctc actgagttta attatattaa aaatatgaca
    13501 taaaggtgtc gtatattata aattataatt gaattagttc aaaataaaat agattaataa
    13561 aaacaaagtg aggtataaag tatgaattat gaaatagtag agataaatga gaaaatagtt
    13621 ataggaatat cgaaggagac tacaaataaa gatggacaag cgataaatga tataggtgaa
    13681 ttgtggaaaa aatttatggg caaagggata tataatgcta taaagggtaa aaagaatgac
    13741 aagacaatag gtctatatac agattaccag ggtgatttta caagtccgta tagctttgtt
    13801 gcatgttgtg aagtaaatag taattctgat aaagagaaga atttagaaga attaaacact
    13861 aataatactg ttggagaaag tattattagt aaagttatac cagcaggtaa gtatgcaaag
    13921 tttgttatag taggaggtca aaaagaagta ggagattttt ggtttgagtt ttggcaaatg
    13981 gattttgata ggacttatat aagtgatttt gaagagtatc aatgtaatac ttttgataca
    14041 aagaaacaag aagttcatat ttacatagga ataaagtaga tttgttatta ctatattgaa
    14101 tgaagtattg agtatagtac atttattata gatagtttta ttatttataa ctataagcag
    14161 taaatctgta aaagatttac tgctttttta gtaattaata gtatcagtat cactttgaat
    14221 cttcagacat attcttcatt taatattgtt tattgataaa tgatacttgt aaatataatg
    14281 atttgttatt taatttaaaa tatgaaaaaa agtattataa acacattaga atacttctgg
    14341 atttatgggt atacaatttg aaaaatataa aaaataatgt ataaagctaa atctaattta
    14401 atcgataata catatagaga aatataatta agtaaataaa caactatcta ttagatattg
    14461 agaaagtcaa atcctatatg aatctacaga aatgatttta atgaatctga aaaatttttt
    14521 tatacaaaca taaaaaaggt ataaagttgt tttattataa tttaattact tttgataact
    14581 ttttatgttt tatacaatta tatttttaat aactcgtcaa aacgccaaac atatattaca
    14641 atagatatat aagttttttt gttgacaaat tatgttaaga gaattaagta taatatggca
    14701 taaataatta cttttattta tcaatgaaat gtatttattg tgtagcgaaa aatagtcaga
    14761 tagtatagac aataactgaa gataaaagcg tttattgttg tacatatgga ggagttagtt
    14821 ttatggaaaa taaaattttg atagtagatg atgctatttt tatgagaatg ataattaaag
    14881 aagctctgag taaaagtggc tataataatt taatagaagc aaatgatgga gaagaagcgt
    14941 gtaatatatt taaatatgaa aagcctgatt tgatgttgct tgatattaca atgccaaaaa
    15001 aagatggttt agaggtgcta agagaaataa aaaagttgga ttcaagtgct aaggttgtaa
    15061 tgtgctctgc aataggacaa gagaatatga ttgtagaagc cataaaactt ggagcgttag
    15121 attttatagt gaagcccttc aaaacagaaa cattagttaa agttgtagat aatgctttaa
    15181 aataaaagtg taaatataca ctaatacaat gaaaagcaaa taaatgcaat aataaaggga
    15241 ggggtttata ttataaatta tttagaactt gatgatgttc atatagatgc tcttagagaa
    15301 attggtaata taggctctgg gaatgcaatt actgcacttg catccatgat aaatagcaat
    15361 gtagaagtat taatacctat ggtcaagata cttgaatata atgaagcgac aaatttgctt
    15421 ggtggtccag aaaacaaagt agtatgtatt ttactcgata tgaaaggtga tatcaatgga
    15481 atgtttatgt ttttgttgga tgaatcaata actcaactta tgctaagttc tctttttaat
    15541 aaagaagaag cttttttaga tgagattgag gcaattgaaa tttcagcaat taaggaaata
    15601 ggaaatatta tggcaagttc atatgtaaat gctattgcat ctatgttaaa catgactata
    15661 agtgtttcaa ttccagatat atgtattgat atggttggag ctgttctaaa tgtgcctatg
    15721 ataagatttt cagatgttgg tgacaaagtc ttatttatag aaaataaatt taaaatgagt
    15781 gataattatt ttactagtca cattttgatg attccagaaa tgagctcact tagagaaata
    15841 ctcgtgaggt tgggattaga agtatgaata gagagatagt agtgaacatt gcagatatga
    15901 agatagccta tagaccaaat gttttagtta cttatgcact tggttcttgt gtaggagtat
    15961 gtttgataga taaagtagct ggaattggtg gaatgttaca cgttatgttg ccatatagta
    16021 aagatgctat taacattgaa aataagtgta aatttgctga cacaggaata aatgagctca
    16081 ttaaatctat ggagaatata ggtgcaaata gaatatatat aaaagcaaaa attgctggag
    16141 gtgctcagat gtttttagga agtagcaatt cagtaattgc aagtatagga cataggaatg
    16201 ttgaggcagt aaaaaaaact cttttaggat taaatattcc aatattagct gaggacacag
    16261 gtatgaatta tggtagaaca atacgttttt acactgagac gggtgaatta cttgtagatt
    16321 caataaataa aggtaagaag aaattataat atatatttca tagaaagtgt attttaggag
    16381 ggaggtatta aatgagattt ccagatgacc catatttaga ttatggacta attgatgaag
    16441 gaattaagta tctaaagttt aaagtagatg agcaagattt tggggttgaa cttgaaaagg
    16501 taatagaaat tactggaaat caagaaatta tatttattcc agaactacct acatattcta
    16561 aaggcataat aaatctaaga ggaaaaatca ttccgataat agatatgcgt ttgagactta
    16621 aaaaagatga attaatgact acaaattgca tgtatatagt agtaactgaa ataaaggact
    16681 tggtagttgg atttgtggtt gatagagttg atgatatagt aagcttagaa aaaagtaaaa
    16741 tatcacctcc accaagagtt actatagaat attcggatta ttttgtaagt ggagttggag
    16801 aattagaagg taaaataatt actttactag atttagagaa acttttgaca gataaagatg
    16861 aagttttaat agatgaatta atcaatgggt ttgattgcac tatcatgtaa catagggatg
    16921 tagttggagt gttattataa aaagattgtg atgagaacat ttaagaatat agtggtataa
    16981 ttattaagta taaaaatttt aaatcattaa aatttgaata tattatgttt aagagaggtt
    17041 tgtatatgaa aaataagcaa cagggaacaa ttttagtcgt tgatgatagt gttctgatgt
    17101 gtgacttaat agataaaaca cttacaaaag ataattttaa ggttaaaaaa gcatttaatg
    17161 caatagaggc aaaagaattt attgaagaat ttataccaga cattattttg cttgatataa
    17221 ttttaccaga taactctggt tttgagtttt gtaaggaagt aaaatctaat gctcgtacag
    17281 cagatatacc tataattttt ataacttcaa aaaatacaga tacagacata gtaaaaggat
    17341 ttggtgtagg agcaattgac tatatggtga aaccttttag tatgacagaa ttaaaagctc
    17401 gtgttatagc tcatcttgaa gtaaagaaat ctcaagataa attaaaaaag ataaataatg
    17461 aactggaatc ttctcttgaa aaattgaata gactcgttgt aagagattat cttacaggtc
    17521 tttacaatag gcgacatata attaatcagc ttatggagca aagaagaatt aataaatata
    17581 gtgaaatatc aatatccctt atccttggtg atattgatga ctttaaggtt atcaatgata
    17641 cttatgggca tgaagcaggg gatttcgtgt taacagtaat atcaagtata ataaaaaaga
    17701 attgtagaca aattgatact gtatcaagat ggggtggaga agagtttttg ataatgctca
    17761 caaatactcc aatagaaggt gctaagatac tttcagaaag aatccgtaaa gagataaagg
    17821 agtttgattt caactataaa gaacataaaa taaagtgcac tattacatta ggagtagtag
    17881 aagtaaactc aaatatatca ttagaagaaa acatatcctt agcagataaa gcaatgtatg
    17941 agggtaaaaa cttaggaaaa gattgtagcg tagtgagtac aatgaaagga ttaaaaaaga
    18001 tataaactta ctttatgaag gagtacagat atgaaaagag aagtaaagct ttctaaatta
    18061 gcaattgtat tgttgattat tatagtattg gctgttctag cagggatttt ttcttctatt
    18121 tcatcttata aagcatttga aaaagtagaa aaaagtatta atgatatgaa taatttgagg
    18181 actcaaagtc agtatataaa agatacaact agagagataa gtgatgtatt aagaagatat
    18241 gtatttacag gagaaaaaaa gaataaagat atttatgaaa aagaattata cggaaaaaca
    18301 agagaatcag ctttaaacaa attagaaaaa attggtctta caaagagcga gtcaaataaa
    18361 atcttagaag caaaagaaat atcagattct ttagtaccaa cagaacaaag tgcaataaag
    18421 gcagtcgaaa gtaatgataa gagtaaagct caaagtataa tttttggtag tgactatata
    18481 aactcccaag agaaaatgga ttctttaata aatgaatttc aagaggatat agttacccgt
    18541 tcaaattcca aaattgtaga agtaaaaaaa gaattacaaa gtctaatatt aagaacaata
    18601 tttatacttt cccttttaat aatattgaca ataatagaat atacagtaat aaaattaaaa
    18661 atagtgattc ctattaataa agtagaaaaa cacttttcaa gaattgcatc tggggactta
    18721 agaacaagta tagacgttga agaaagtaaa tctgaaatag gactattagt gagttctgtc
    18781 aaaaagatgc aaagtatgct tttaaattac atagacctta tagacagtac attaactagt
    18841 attgcaaaag gagatttaag agtagagata gaccaagaat ttataggtga ttttaaatca
    18901 attaaaatat ctttagaaag tataataaac tcttttaatg gtgatttttc tgaaataaat
    18961 ttatcagcag agcaagttgc aagtgcttca gaacaagttg cagcaggtgc acaagctctt
    19021 tcacagggtg ctactgaaca agctagttca gtagaagaat tagcttccac gattaatgag
    19081 atatcagatg gaattaagaa gtgtgcagat agtgctagtc ttgcatgtga ggtatctgac
    19141 caatctgcat cacaagttgg ttttggaagt agatgtatgg atgatatgat gacatcaatg
    19201 ggtgaaataa gtgaaaaatc gagtgaaata tcaaaaataa tcaaaacaat agatgagata
    19261 gcattccaaa ctaatattct tgcattaaat gcagctgttg aagcagcaag agcaggtcaa
    19321 tatggtagag gatttgcagt tgtagcagat gaagttaggt ctttggctgg taaaagtgca
    19381 aaagcagctc aaaatacagc tattctcata gaagaagcta ttaaaacagt agaaaatggt
    19441 gtcagaattg ctaaagagac tgcaaatgca ttggacttgg tagtaaatgg tgtagaaaaa
    19501 actactaatt atattaatgg tatttcttcg gctgtacata gtcaagaagt agcaattaca
    19561 cagattttat ctggaataga gcaaatttcc atagtagtcc aaatgaactc agcaacagca
    19621 gaggaaagtg cagcagcctc agaagagatg tctgctcaag ctcaaatttt aaaagaatta
    19681 gtagaaaaat ttcatctaaa ggaaagtgaa gtaagagaat catcatgggt ttaaattatg
    19741 aggtgaatat tgaagtaaga gaatcatcat gggtttaaat tatgaggtga atgttgaagt
    19801 aagagaatta tcatgtattt aaattatgag atgtatattg aaataagaaa atgattgtgt
    19861 atttaaatta tgaggtacat atagtgagat aagagaatca tcaaggattt aaattatgag
    19921 atgcatatat aatagaaaat tatttgtagg tcgtaggtga taaatgtgaa ttatattgaa
    19981 ccgagtatga gacaaatgtt agatatgttt atatttgaga caagtttatt actcgaacaa
    20041 ttagatgaaa ttatgattca aatagaaaaa gagaaagtat ttactcataa caacattaat
    20101 gaaactttta gaataatgca tacgataaaa ggttcagcat ctatgatggg gttagacaat
    20161 atatctgctt tagcacatag tgtagaagac ttgttttatg ctatacgtga aggtaaaata
    20221 gaagttttag acaatgacct attatttgat ttagtctttc aatcattaga ttttataaga
    20281 agagatatag atgagttaca atcaagaggt tgtatatctt ctgattactc taattttatt
    20341 gaaggaattg aagagtatgt gagtactttg gaattatcaa gttctatgaa atcaaaggat
    20401 aatgcaaatt tagaaagtaa agacgaaaaa gaagtaatcg attcaaattt tgaatacaaa
    20461 gaagactctg atgtagtaaa aataatcttt gaacaaggtt gcatgatgga aaatgtgaga
    20521 gctttcatgc ttgtatttga acttagagat tactgtgaat ttatagattt ttatccaaaa
    20581 gatgtagaaa caaatgcaga gtctacagag tatattaaaa gttatgggtt ttatatacaa
    20641 tttgtagcta aagaggataa ggcaatagtt tataaaacta tagaagataa tctctatgta
    20701 aaagaataca taaatattag taaaaaagaa atagaaatga aaaatacatc aaacaatgat
    20761 gcagtagaaa ataaattaaa aaataataat aaagtaagta aaagttatca aaataactat
    20821 acagaagatg agatacaaga agatgtttta aatgaaaaaa ctgatatgga aaaagatagt
    20881 ctaagtgaag aaaatgaaca tttaccaata agtaagcaga gtattatgag tgtaaattta
    20941 gaaaaattgg acttacttca aaatatagtt ggagaaattg taattatgga atcaatggtt
    21001 gtaaatagcg taaattctac aggtgttgat atagataatt tcaataaggc aacaagacaa
    21061 ttacacaagc ttacagatga attgcaagat atagtaatgt ctatgcgtat gattcaaatt
    21121 gctggtgtat ttcaaaaaat gcatagaatt gtacgagata tgaataaaaa attagataaa
    21181 aatgtagagt taataactat aggagcagag acagaggtag ataagagtgt aattgataat
    21241 ttagcagaac cctttatgca ccttataaga aatgctatgg accatgcaat agagcctatt
    21301 gaggagcgta tagcgaaagg taaagaagaa gtgggaaaga tagttttatc tgctgaaaat
    21361 ctaggcggag aagctgtgat aagtatttca gatgatggta gaggcattat gagagataag
    21421 attttggcaa aagctaagga acaaggtctt cttaaacatc aaagtaatga gtatactgat
    21481 gacgaggtca attctttgat tatgactcca ggtttttcta caaaagaatc tataactgaa
    21541 ttttctggaa gaggagtagg catggacatt gttcgtaaaa gtattgaaaa agttggaggt
    21601 tcaattgaag taaaaagtaa acaggatgaa gggacaacct ttactataaa aataccatta
    21661 actctttcta tagtagaggg tatgaagttt aaagttggag attcactatt tactttacca
    21721 atatcatcca taagaagaac atttaaactt acagacttaa atcaaataat tacagaagga
    21781 aataagacag agttaattac aatatatgga gcctgtattc ccattataag gttacataaa
    21841 atatataata ttcaaacaaa agtaaccaat ctgatgcatg gtaatatgat aattattgaa
    21901 aatgagcata aagcagtatg catttttgtt gatgaaataa taggtaatca gcaagttgtt
    21961 gtgaaaccat ttccagttta ttttaaccga tttaatataa aaaataaggg tttatcggga
    22021 tgtacaatac ttggggatgg aagtataagt ttaattatag atgcagatag cattatagaa
    22081 atatattagg ggatttaaag tatggctaaa gatgaaatta ttaaacaagt attaacattt
    22141 tatgtcaatg atgttattta tggaatagaa ttagaaaatg tgattgaaac cataagattt
    22201 caatcaataa cgtatgtacc atgtcttcca acatatataa gtggagttat taatttgaga
    22261 ggaagaataa taccagttat taatatgcat ataaagtaca atcttcctaa agctgattat
    22321 aatgagagaa cttgtataat aattacaaag gttgatgagt atcaagttgg aattattgta
    22381 gataaagttg ttgatgttat tcatatagaa aatctaaatt tactagagac gactaattct
    22441 aacaacacta atattaataa agatataaaa gagattgcga aagttgaaga taattctata
    22501 ttaattttag atattcgaaa gtttttgatt aatagcatat agttagttct gataaattag
    22561 caggaggaag tttttgcatg ataaaactca cagatgaaga atttatagtt cttgtaaacc
    22621 atgtaaaaaa agaatatggt attgatttaa gtaaaaaaag agcgttaatt gaaggtcgct
    22681 tatacaatac aatgatagag aaaaaactta gttcttttag tcaatatatg aatctattat
    22741 ttaaagataa gacaggaaat gaagctataa acctaataaa tagattgagt actaaccaca
    22801 cattttttat gagagaacct caacattttg agtttataca gaatagtata cttccttttt
    22861 gggaagaaaa taataaaata agaaacataa atatatggag tgcaggatgt tctagtggag
    22921 aagaagcgta ttcaatagct atgactttgg atgattattt tggatataat aaagagttgt
    22981 ggaacattaa aatcatagcc acagatattt caactaactc acttgaaaaa gctaaaagag
    23041 gtatatacat agaatcaaat gttaacaatg ttcctgagtt atggaagaag aagtatttta
    23101 tagacaacaa ggatggtaca tttaaaattt gtgataagat tagaaaaagt gtaattttta
    23161 agccgtttaa tttaatgaat gatttctcat atcaacattt tgatttgatt ttttgtagaa
    23221 atgttatgat ttattttgat ttaaaaacaa gagaagaact tataaataaa ttttataatg
    23281 ctaccaaaaa agatagtttt ttgtttgtag gacatgcaga agtaatcaac agaagtagta
    23341 ctaaatacaa atatgtaaaa cctgctatat ataaacgata aaaaggtatt tttgatatat
    23401 gattttttgg aggcggtaat gtggaacgtt ttaataatat ggttattgct ataggcgcat
    23461 ctgtaggtgg tacagaggca atacttgaaa ttataaaaga cctaccaaaa tcaacaccag
    23521 ggattgttgt agttcaacat atgccagcta tatttactta tatgtatgca caacggcttg
    23581 acaagcagtg tattatgaat gtaagagaag ctaagaataa tgatagagtc gaacaaggaa
    23641 atgtgttaat tgcacctggg ggttaccaga tgaaattgtg tacggataaa caaggatatt
    23701 atgtaacttg tgaaaaggga gaaagaataa gtggtcactg tccatcagta gacgttttgt
    23761 ttgattcagt agcagaagta gcaggtaaaa attcaatagg aataatactt acaggtatgg
    23821 gttatgatgg agcaagtgga cttttaaaaa tgaaagaaaa tggttcattt acaattggac
    23881 aggatgaaaa ttcttgtata gtatatggaa tgccaatggt agcttttgat agaggttctg
    23941 ttatgttaca attacctctt ggtgaaattt caaactgttt gataagacat attaatattt
    24001 taaaacaaaa agattaattt tttataaata aaaattaatc ttttattaaa ttatatggat
    24061 aaaaaaccct agaaactcca aatataataa gagagattta tatttaggag gaaaatgata
    24121 atatgaaatt ttttaaagtc gatacaaaag ctaaagtttt aagatatgta tttgttttaa
    24181 cattgactat aatggctgtt ttggcaatta aacatgacta taaatataat aatttaagga
    24241 aagatgcttc aattaattta aatagtaata aggaagcagt gactgttatg agtaaccagt
    24301 cattaccaaa gggtaataca aatagtaaac aaactttatc agtaagtaca ataggaagta
    24361 aaacaatagt gaaaaatgat gagtatttag aaagtactat aaacatgcct gtaataacaa
    24421 attcaaataa aattgttgaa aggtctacaa atgataagat aaaaaatgac atatttgaat
    24481 tttataataa atcatataaa gaagcaaagc agtatcttaa ggataatcct gatgagaaaa
    24541 ataagtttgt tgcaaatgta gactttgaac ttaaaaagaa tactgattct gctcttagta
    24601 taaaagtgag atattatact tatagtggtg gagcacatgg attttatcaa gatatagcat
    24661 ataatgtgga tatgagaact ggaaaatttt tagaattaat ggacttgttt aaggataata
    24721 ctaaatataa agaagttata gatgaagaaa ttaaaagaca aatagctgaa cttgaaaaaa
    24781 aggatgaaga aaatatagga atatataatt ttaaaggtat aaaagagaat caaaactttt
    24841 acttgcaaga tgagaatcta gttgtatatt ttgatttata tgatataact ccatatgcag
    24901 cagggatacc agaattttca ataaataaag cgttgataag ttctatgcta aaaccagagt
    24961 atgtagattt atttgatttg aaatagaaga ttaaattaaa tgttatgcta taatatttag
    25021 catagagact tattaaaatt gggggtataa aaatgctact tttaaaaaaa gacgacataa
    25081 aaaaagtatt tacaatgaga gatgcaattg aagctgataa ggaagctttt agaatttatt
    25141 gtgaaggaaa gagtgtaaat cctttgagaa ccaatatatc tgttccaagt caagatgcta
    25201 gtatgttatt tatgccagga tatgtggaag aattaggctg tgggggaata aaaattgtat
    25261 ctgtatttcc taaaaatgct caaaaaggca aaccagtaat tccagcaaca gtactattat
    25321 tagatggaga aactggagaa gttagtgcag tattggatgg tacttatgta actcagattc
    25381 gtactggagc agcctctgga gcagctatag acgtattggc taaaaaagat tctaagattg
    25441 gagctttgat aggtctagga ggacaaggtg agccacaact tgaagccatg atagaagcta
    25501 gaaatttgga tgaggtaaga gtttttagta gaaacaagga aagtagagaa aaatttgcag
    25561 aagaaatgaa cttaaaatta agtaaatata atactaaaat agttgcagtt gaatcatctg
    25621 atgaagccat aaataacgca gatgtgatag ttttagctac tccatcaaaa caaccagtac
    25681 ttaatggaaa cttagtgaaa aaaggagctt taataagtgc agtaggttca tatatgccag
    25741 atatgcaaga gttgtgccca aactgtttaa ctagagcgtc taagatattt tttgaatcaa
    25801 cagatgcagt tttatctgag tcaggagata tattaattcc attaaaagaa ggtaagataa
    25861 gtaaagatga ttttagtggt gatttaggga atgttataaa cggaactatt cctggaagag
    25921 aagatgatga tgagataatt gtatttaaaa ctgttgggat aggtgtacaa gatgtcgtaa
    25981 cagcaaaaag aatatacgat aaagcaaaag aaaacggtat tggaatagac tggaagtggt
    26041 aaaatattca aaataattct atagttagac aaatttatgt tataatgttg ataaataaat
    26101 tttttattat tttaattgag ggaggatgtt tgtatgaaaa tggtaaaaaa gttaatatta
    26161 tctacgatta tggctctagt tttagttcta actatagggt gttctaatgc agaagaaaaa
    26221 gtaaagtcga attttgaagc taatttagct gcattagaaa tgtataagta tgatgaaaaa
    26281 ggaatttctg atgcattaac agaagctgaa tataaacaga ttcaaactta taacagcaag
    26341 gacaaggttt caatgattgc tcaattgaca cctctttatg gtaaagaaaa tgctgaaaag
    26401 atatacgaaa caattgttaa attaattcaa ggtgttgaaa agacagtaac agtggaatct
    26461 tctagtaagg ataagataac tttgaaagtt gtaagtaaag ctttagatac tagtaaagta
    26521 atgagtgaag cgaacacttt agtaaaatct tatgtaactg atgaaaagaa ttacgataaa
    26581 aaagaaaaag aagttcaaga tgatatgaca aaaatgatta tagaccttta caataagaaa
    26641 ccaactaagg aagttacaac tatgattaca tatactaaaa aagatggtaa atggcaacca
    26701 cctagtaata tacaaaagtt atgtgaagga atatatacaa tttaatttat tttaaagaca
    26761 caaaactatc ttttaagcat ataataaaat gtttggaaga tagtttattt tattttcaaa
    26821 gtataaatta tat
//
LOCUS       DAGSHT010000032        26710 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGSHT010000032 DAGSHT010000000
VERSION     DAGSHT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.033"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            445..1920
                     /locus_tag="MJB72_001251"
     CDS             445..1920
                     /locus_tag="MJB72_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY8262376.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            2007..2420
                     /gene="speD"
                     /locus_tag="MJB72_001252"
     CDS             2007..2420
                     /gene="speD"
                     /locus_tag="MJB72_001252"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBY8262377.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2450..3301
                     /gene="speE"
                     /locus_tag="MJB72_001253"
     CDS             2450..3301
                     /gene="speE"
                     /locus_tag="MJB72_001253"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBY8262378.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHVPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3291..4169
                     /gene="speB"
                     /locus_tag="MJB72_001254"
     CDS             3291..4169
                     /gene="speB"
                     /locus_tag="MJB72_001254"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBY8262379.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISNKIK"
     gene            complement(4349..4549)
                     /locus_tag="MJB72_001255"
     CDS             complement(4349..4549)
                     /locus_tag="MJB72_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY8262380.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            5017..6507
                     /locus_tag="MJB72_001256"
     CDS             5017..6507
                     /locus_tag="MJB72_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY8262381.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            6787..8283
                     /locus_tag="MJB72_001257"
     CDS             6787..8283
                     /locus_tag="MJB72_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY8262382.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8428..9354
                     /locus_tag="MJB72_001258"
     CDS             8428..9354
                     /locus_tag="MJB72_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8262383.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            9472..9663
                     /locus_tag="MJB72_001259"
     CDS             9472..9663
                     /locus_tag="MJB72_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262384.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            9886..10236
                     /locus_tag="MJB72_001260"
     CDS             9886..10236
                     /locus_tag="MJB72_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262385.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            10498..12003
                     /locus_tag="MJB72_001261"
     CDS             10498..12003
                     /locus_tag="MJB72_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBY8262386.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            12393..13490
                     /gene="dinB"
                     /locus_tag="MJB72_001262"
     CDS             12393..13490
                     /gene="dinB"
                     /locus_tag="MJB72_001262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBY8262387.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            13753..14889
                     /locus_tag="MJB72_001263"
     CDS             13753..14889
                     /locus_tag="MJB72_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262388.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            14882..16429
                     /locus_tag="MJB72_001264"
     CDS             14882..16429
                     /locus_tag="MJB72_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY8262389.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            16763..17629
                     /locus_tag="MJB72_001265"
     CDS             16763..17629
                     /locus_tag="MJB72_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY8262390.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            17770..18912
                     /locus_tag="MJB72_001266"
     CDS             17770..18912
                     /locus_tag="MJB72_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY8262391.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKLEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            19296..20534
                     /locus_tag="MJB72_001267"
     CDS             19296..20534
                     /locus_tag="MJB72_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262392.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            20563..21450
                     /locus_tag="MJB72_001268"
     CDS             20563..21450
                     /locus_tag="MJB72_001268"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBY8262393.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            21496..22125
                     /locus_tag="MJB72_001269"
     CDS             21496..22125
                     /locus_tag="MJB72_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBY8262394.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            22130..22594
                     /locus_tag="MJB72_001270"
     CDS             22130..22594
                     /locus_tag="MJB72_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBY8262395.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            22844..24211
                     /locus_tag="MJB72_001271"
     CDS             22844..24211
                     /locus_tag="MJB72_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8262396.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            24411..25685
                     /locus_tag="MJB72_001272"
     CDS             24411..25685
                     /locus_tag="MJB72_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBY8262397.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            25803..26522
                     /locus_tag="MJB72_001273"
     CDS             25803..26522
                     /locus_tag="MJB72_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBY8262398.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
BASE COUNT        10432 a         2760 c         4677 g         8841 t
ORIGIN      
        1 ttaaaaaatt taataaattt tatattaaaa ttaaaataca aaaataggaa aattatttag
       61 tccaactaaa ccaagaattt tgatatgtga aataagaata atataaaagt ttagtaataa
      121 aatacttata aattaaaaat agtaaactca aaagcttact aaaactcaaa aaagtaaaaa
      181 aaccaaaata ataaaaaata agaaagttta taaatactaa gttttaaaaa aattaatata
      241 aacaacaaaa aattaaaaag tttataaata ttgatatagc taatgtatat gtttttgagt
      301 aacttcaaaa aaacacttgt aaaaatgctg ggatgttaat ataataaata tcatgagttt
      361 aataaaagta aaaataaaaa atagaaataa taaataataa atattaaaaa tatttgaata
      421 tagagaggag atgaatttga aatcatggat agcgagtttg caaatgcagt tgaattaagc
      481 tatgagtgtg ctccattatt agagtcgtta aaggaatact cagagaaaga tatagcttgt
      541 tttgatgtgc ctggacatgt aaaaaataga ggtgtagcta tattaaataa atatctaggt
      601 gaaagtctca tgaaaatgga tataaattca tcaccaacta tggataatgt atctgcgcca
      661 aatgggatta taaaaaatgc acaagatttg ctagcacaag catatatggc tgatgaggca
      721 ttctttataa cgaatggcac aactcaagca atacatgcaa tgattttgag tgttataaaa
      781 ccaggtgaaa aagtattact tccaagaaat atacataaat cagtaataaa tgcgttaata
      841 ctttgtggag gaataccaat atttatacaa ccagaatttg atgaaaaact aggtataagt
      901 ttaaatatta ctcttgaaaa agttagaaca gagatagaaa aagattgtaa tataaaagca
      961 ttatttttcc ttaatccaac ttactatgga gtttgtgcag accttgagag tattatagaa
     1021 ctatgccata aaaataatgt tttagtgtta gttgatgaag ctcatggtgc acattttcca
     1081 tttcatttag atttacctcc ttctgcaata agtttgggag ctgacatggt agctgtaagt
     1141 atacataaaa ctggaggagc attgacacaa tcttcagcac tcttattgaa tagagataat
     1201 gtaagttttg aaaaggttct tcaatcaatc aatatgctac aatcaacatc agcatcttat
     1261 ttgcttatgg caagtataga tggtgctaga gttaatctag ttgagaatgg tgaaaagcaa
     1321 ttgtcaaaag cattaaatct atctaggtat gcaaaatcta aattgaacaa aattgatgga
     1381 ataaaagttt tatctacaga aatattgaaa caaaaaggtg ttaaatttat agatgaaaca
     1441 aaattgtgta taaatgtaaa agaattaaat ttgactggat ttgaagtata cgatttatta
     1501 tataaaaatt tttcaattca agtagaattg ggagattcat ataatatact ggcacttgtg
     1561 tccattggaa caaataaatc agatatagat agattagtaa aggcactttc tataattgca
     1621 aagatttata gaaaagaatc aacattaaat gaatttaata tggttcaaat aaatccaatt
     1681 attaaactta atccaagaga agctttttat gcaccaaagg aaagtgtaga aatcaattca
     1741 tgtatagata gaatttgtgg agaatcaata atggcatatc caccaggtat accgattatt
     1801 gctccaggag aattgataac agaagaaatt atggagtata ttattttctt aaaaaatagc
     1861 aatgcgtatc ttacagatgt acaagataaa aacttagata gaatactagt aataaaataa
     1921 attactttat tttgcaaggg caagtgtttg ttaagtagag tttatcaaat agaacttatt
     1981 aaaagtataa aggagttggg gttgaaatga aatttgaaac actaggaaga catatactag
     2041 tagaatacta taattgtgat gaaaacgtac ttaaagaccc acgtcttata gaaaatttta
     2101 tgaatgattc agcattaaat gcaaaagcga caattgtgga ttctgtattc caccatttta
     2161 atccttgggg ggtttcagga gctgttataa ttgctgaatc tcatttgaca atacatacat
     2221 ggcctgaata tggttatgca gcagcggatt tttttacttg tggtgatata gacccatgga
     2281 aaagttttga attattagag caacttttaa agtctgaaag aagcgagtct acagaaattc
     2341 caagaggctt aactacaaaa attaaaaaat attcaaaaaa agatttaggt aagattactc
     2401 ataaaccaga agcagtataa aacatacaag agaaagtgag gattattata tggaactttg
     2461 gtatacagaa gaatggacag aaaatgtaag attttctata aaagtaaata aacatctatt
     2521 tgaaggaaag tctcaatttc aaaggataga tgtgtttgat agtgatgaat ttggaaaatt
     2581 tctaacaata gatggattaa tgatggtaac atataaagat gaatttatat atcatgagat
     2641 gataactcat gttcctatgg ctacaaattt aaacataaag aaagtattag tcataggtgg
     2701 aggtgatgga ggtacagtaa gagaattaag ccgttatcct caaatagaaa agattgacat
     2761 ggttgaaata gataaaatgg ttgtagatgt atctaaggag tatatggata tatgctcatg
     2821 taaattagat gataaaagag tgagtttata ttttgaagat ggtgttaatt ttgttaagtg
     2881 tgcacatgat aaatcatatg acctgataat agtagattca actgacccaa taggaccagg
     2941 tgaagggctt ttctcaactg atttttataa ggattgttat agaatactga ctgatgatgg
     3001 aattttagta aatcaaagtg aaagtccgta ttttgacttt aatgctaaag agatgaaaag
     3061 agctaataaa aagttaaaac agatatttcc aatatcagaa gtttatcaag cacatgtacc
     3121 aacgtatcca tctggtcact ggttgtttgg atttgcttca aaaaaactta atccagtaaa
     3181 aaatcaagat agaaatggat gggagaaatt aagtttaaaa actaaatatt ataatagtga
     3241 tatacattta ggttcgttta tgttacctca atacgtgaag gagatgctag atgaagaata
     3301 atttctatca tatgaacact tttatgagta tgaacaaaaa ttatgaagaa tctaatctta
     3361 tagtatttgg tgttggattt gatggaacta cttctaacag acctggggct agatttgcaa
     3421 gtagctctat gagaaaagaa ttttacggtc ttgagacata tagtcctttt ttagatttgg
     3481 atttagagga ttataatata tgtgattatg gagatttaga aattagtgtt ggaagtacag
     3541 aacaagtctt aaaagaaatc tatcaagaga catataagat tgttagagat tcaaaggtac
     3601 cctttatgat tggaggagag catttagtta cattaccagc ctttaaagca gtacatgaaa
     3661 agtacaatga tatatatgta attcattttg atgcccatac tgacttgagg gaagaatata
     3721 ataatagtaa aaattctcat gcaacagtaa ttaaaagaat atgggatatt gtaggtgata
     3781 ataaaatatt tcaatttggt ataaggtctg ggacaaaaga agaatttaaa tttgctacag
     3841 aagaaaaaca cacatacatg gaaataggag gaatagatac gtttgaaaat atagttaata
     3901 tgctaaatgg aaagaatatt tatctaacta tagatttaga tgtattggat gcatctgtgt
     3961 ttccaggaac aggtacacca gaacctggtg gtgtaaatta tagagagttt caagagattt
     4021 ttaagattat aaaaaactct aatataaata tagttggttg tgacattgta gagttaagcc
     4081 ctgattacga tacaacaggt gtatcgacag ttatagcttg taaaatctta agagagttat
     4141 gcttaataat atctaataaa attaaataga gtattttata taaatagtat atattataat
     4201 ataagaagtt attttaaaaa agttagttta agtttgacgc ttttctaaag taacttctta
     4261 tttttgtaat taaaaataaa tataataaac aacaaaaaag agccctcgat agctcctttt
     4321 ctaaaatata tatatttaaa ttaaataatt atattctatt tacgttttca gcttgaggtc
     4381 ctctattacc ttgagttata tcaaagctaa ccttttcgcc ttcttctaaa gttttgtatc
     4441 catcattttg tatagctgag aaatgtacga aaacatcatc ttctccctct acagatatga
     4501 acccaaatcc tttttcattg ttaaaccatt ttactattcc gtttttcatt ataagaaaac
     4561 ctccgcatta ttaaaaaata caaactaaaa aaaataagtc tgttctatta atataacata
     4621 aattttaaat aaaagcaaat aaaattttta aaaaaattaa ttacttttag aatttaataa
     4681 aaattgttaa attatacagt gataagtatt gtaaaatttg acgaaaatgt aaatgtagat
     4741 aataaattta atatcaaaaa atgtaacaaa aaggtatgta agtaaaaagt aaaattaaaa
     4801 tatataataa aaaatattat atattagatt gaaaagtaaa aataaaaata gtgaaatttc
     4861 agaaatatca ataatattat ataataaaac aaaaaaacac tatatatatt cgataaaata
     4921 aaatttaaac atataatatt ttatgtatat attaataaaa ggacatattg tgttataatg
     4981 gtatcgtata aaatcattta cgggggtggt attaaaatgt ttcaatttga aacacaaata
     5041 cacaaattaa gacatgatgt tttaactgca atagctagac ttggaaaaga agataatctt
     5101 accagagaag caatggataa tattcagtat gaaataataa aaggtgagaa acctacatac
     5161 agatgctgtg tttataaaga aagagctata atatctgaga gagtaaatgt aagtatgggg
     5221 ttacaaccag gtcaagataa gatagattta gatacaatgg atatagaaga agatgaacaa
     5281 attatatata ttttggaagc tgcatgtgac agttgtccaa taaatgaatt tacagtaaca
     5341 gatatttgta gaggatgtct agcacataga tgtaaagaag cttgtaaatt tggagctata
     5401 acacatgtcg gaggaatggc ctatataaat catgaattat gtaaggcttg tggaatgtgt
     5461 aaaaaagctt gtcaatatga tgccatctca gaggttgtta gaccatgtaa gagtgtttgt
     5521 ccaactaatg ccttaggatt tgatagagaa aatatgaaag ctatgataca tgaggataaa
     5581 tgtttaaact gtggagcatg tatgtcagca tgtccatttg gagcaatatc tgataagagt
     5641 ttgatagctc ctgtagctag aaagttagtt caaaaagaga aaatgtatgc ggtggtagct
     5701 ccagctataa caggtcaagt tgaagcaaat gttacatatg gtcaaataaa aaatgctata
     5761 aaatcactag gatttgttga tgtattagaa gcagcttgtg gagcagatgc agttacagtt
     5821 catgaaagct tagaatttgc tgaaagaatg gaaaagggag aagcttttat gacaaattct
     5881 tgttgtccag gatttgttgg ttatatagaa aaaacatacc caaatcatgt aggaaaaata
     5941 tcttctacag tgtctcctat gatagctagt ggtagattta taaaaagtca tgaaccagat
     6001 gctaaagttg tattcatagg accatgtaca gccaaaaaga gtgaggcagc taagaaagaa
     6061 ttaaaaggag ctgttgatta tgtacttact tttgaagaaa taatggcatt atttgaggca
     6121 tttgatatag acttggctaa gtgttcaaat gaagatatag atgatgcatc aatttttgga
     6181 agaggttttg gagctgctgg tggtcttaca aaagctatag aaaattatct tgctgaaaaa
     6241 ggtattggtg ttaattttga acctgtaaag atttctggtt caagagaaat caagaaaact
     6301 atgactatgg ctaatttagg aaagctagaa ggtaatttta tagaaggaat gatgtgtgaa
     6361 ggtggttgta taaatggttc tggtaaattc ttatcaggtg caaaggttaa agccactttt
     6421 gatagaaaaa attctcaatc aactaagaaa agtgtcttag caaatacaaa gataaaagat
     6481 tttagtaata taaatttaga aaaataaaga taatacaaag caacaaaaat acatattgtt
     6541 aatagagttg ttctaagttg aggtttaact tttggacaac tctattttta tttatatatt
     6601 taattattga acaactaaaa taaaaattat atacacttaa aataaaaatt acatatataa
     6661 tattttgtat ataacttaac aaagtatatg cctatgttat aataatttat agaaataaat
     6721 aaaaaataca atatacataa tagtagaaat atagcaaaaa ggtgtgattt atagggagga
     6781 ttacttatgt ttgaatttga aacgcaacta aaaaggttaa aacatgaagt tttaacagca
     6841 gtagcaagac tatctattga agaaaattta acaaaagaaa atatagaaaa aattccatat
     6901 gaaataatta aaggagaaaa ggcgagatat agatgttgtg tttataaaga gagagaagta
     6961 gttcttgaaa gggcgcaact tgcattaagt ttaactccaa atagtaaata tggagatata
     7021 aatccagatt cagttagttt ggacacagat gagcaaatta tatatatagt tgaagcagca
     7081 tgtgatagat gtccaataaa tgaatttaca gttacagagc tttgtagagg gtgtttggct
     7141 catagatgta aggaagcatg taaatttggg gcaatttctt atataaatgg gagagcatat
     7201 ataaatcatg atttatgtaa ggcttgtgga atgtgcaaaa gttcatgtca atatgatgct
     7261 atatcagaag ttgtaagacc ttgtaaaagc gtttgtccaa caggagcttt ggattttaat
     7321 agaaacacta tgaaagcaat gatacatgaa gataattgta taaattgtgg agcatgtatg
     7381 tcagcatgtc cttttggagc aatatctgat aagagtttga tagcacctgt tgcaaaaaaa
     7441 cttgcaaaga aagaaaatat gtatgcaata gtagctccag ctataacagg tcaaatagat
     7501 agtaatatta catatggtca gttaaaaaat gctataaaat cactgggatt tgtagatatg
     7561 atagaagctt cttgtggagc agatgcagtt acagttcatg aaagcaatga acttattgaa
     7621 agaataaaat caggagataa ttacatgaca aattcatgtt gtccgggatt ttttaagtat
     7681 atagagaaga tgttcccaga tgaaaaagat agaatatcat ctacagtgtc tcctatggta
     7741 gcaacaggaa gattagtaaa aaaaatagat tctgatgcta aagttgtatt tataggtcca
     7801 tgtacagcta aaaagagtga ggcaacagag aaaagtttaa aagattcagt agattatgta
     7861 cttacttttg aagaactaat ggcattattt gaagcattta atatagattt atcaaaatgt
     7921 tcaaatgaag atataaatga tggttcgatt tttggaaggg gatttggagc atctggtgga
     7981 cttgccaaat cagtacagaa ctatattgct gaaaaggggc ttagttttga ttttgaacct
     8041 ataaaagtat ctggtccaag acaaattaaa aagacaatga caatggctaa attgggaaga
     8101 ttagaaggta attttataga aggaatgatg tgtgaagaag gttgtattaa tggagcaggt
     8161 aagtttttat caggtgtaaa agctaaaaat acttttgata gaaaaaatgg acaatctact
     8221 aaaaagagtg ttttatctaa tgataagatt aaagaatttg aagatgtaaa tttagaaaga
     8281 taattgtttt ggtacagtca tttttttatc atgtttttat catgccacaa atatgcctaa
     8341 aatttgccct ttttatatgt gtattttaca gagttaaggt tgctatacta taaatatgta
     8401 ataaataaaa tttataggag cttttggatg aggaaaatag tattaaaaga tatcctgttt
     8461 ttagttacta cattatctat aatagtaata ttattgcttg taacaaatat aactactaat
     8521 agtaagataa aatcaacaag tgaatcttat ttaaaaagtg acaaaaatag tgtgaggcaa
     8581 aatggtatcc agaggagcac aaagagtagc acaaaaaata ctgcatttaa gactactata
     8641 ctaaacactg gaaagtctga ttgcatattg gttgagatag gtaacaaagt cataatgatt
     8701 gatacaggag aagataaaaa tggaaaacaa atagtggata ggttaaaaga aaaaggaata
     8761 aatacacttg actatttgat tttaactcat ttagataagg accatatagg tggagtagat
     8821 agtgtgttat ctagtgttaa agtaaagaat attatacaag caaattataa gaaagatagt
     8881 aagcaatatg atgaatatat tgactcactt aaaaaagctg atatagaacc agtgctacta
     8941 aaagataata tgaatattgt aatcaatagt gcagaaataa acatacatcc ggcttcaaaa
     9001 agcaagtacg agagttcaaa tgattattca ataatcacta atataagcta tggagcacat
     9061 aagtttttat ttgcaggaga tgcagaggaa gaaagattgt cagagtttat aaatggaaat
     9121 actttaaaat atgattttgt taaaatgcct catcatggaa gatatgacaa attaactgaa
     9181 acatttttag agtctatttc accccaatat gctgttatta catgttcaga gaaaaaagaa
     9241 ccagaggaag gtgttttaaa aatattggaa agattgaata taaaaacatt tttgacttct
     9301 aatggagagg tagttatcaa tagtgatggt aagacattga gtgtgaatca ataatttttt
     9361 taaaaatatc taaaaaaatt taaaaaaatc attagcaaat attacccctt aaacatagta
     9421 tattttagga gctgaaactc cacatacaaa atgactagga ggtaacatat tatgttggat
     9481 agattttttg gtgaaggcgg atttggtgga tgtgcttggt ggataataat attattcttc
     9541 ttattcttag cattccaaga ttgttgggct gaaatggact tatgtgcatg gatacctttc
     9601 ttaatattat tattaatact ttgtgaatgt ggtgacgttt ttgatggagg atgtggatgc
     9661 taaaaaaatc aatttagatg atttaataaa gcttatggtg ggtataaata aaataccaaa
     9721 ccaatcacaa tctagtgttt gaaggtagtc ggaaatcccc cgctaccttc ttttttttat
     9781 tgttaaaatt tatttttgaa ttatttcatt tacatataat aatttttacg ataaagtctg
     9841 atataattaa aaagaaaata aaatttattt ataggagatg taaaaatggc tatagtggat
     9901 aagattttca atgagataaa acttatggtt gcaagacttt tattgggaaa aaaatacagt
     9961 gaatatgaag aaaaagattt agtgtataat acagaagaag aagttatact tataacttta
    10021 aaaagattag tttttcaggg aaatgtaaac gaagcagaag aaattttatt tgataaagct
    10081 aagagtgtaa atagtgaaaa tatgcagtat atagcaatag aattttatac tatgcttatg
    10141 gaaaagactg atgaagaatt agaagctatg aatttttcta aacaagaagt ttatcaaggt
    10201 atagaagata ttagaaaagt attgaattta gaataaaaga tatatgaaaa tattgaaata
    10261 aattaatgct aaggtgagaa tattatactt atatataaaa atatagtatt cttattttat
    10321 tagttatatt aggtatatct tatttaccat aaaactatct tgaaaaaaat taatcttaat
    10381 tgtataatat tactttgagt gaaaaagtat taacattttt ttataagatg ttttactaaa
    10441 aaaatatata taaatatact gttatcaata agaaatttaa tttagtgagg tgttaagatg
    10501 aaagtaggat ttgaccacaa aaaatattta gaagagcagt ctaaatatat cttagaaaga
    10561 gtaaataatt ttgacaaact ttatttagag tttggaggaa aattgatggg agaccttcat
    10621 gcaaaaagag tacttccagg atttgatgaa aatgcaaaga taaaagtatt acaacatatg
    10681 aaagaaaaag ttgaagttgt aatttgtgtc tatgcaggag atatagaaag aaacaaaata
    10741 agaggagact ttggtatcac ttatgatatg gaagttttaa gattaataga tgatttaaga
    10801 ggttatgaac tagatgtaaa tagtgtagta attactagat ttgaaggaca acctgctaca
    10861 actgtattta taaataaatt agaacgtaga ggaattaaag tttataagca ttatccaaca
    10921 aagggatatc cgtcagatgt tgagactata gttagtgatg agggatatgg agcaaatgaa
    10981 tatatagaaa ctacaaagcc aatagtagta gtcacagcac caggacctaa tagtggaaaa
    11041 cttggtactt gtctaagtca gttgtatcat gaaaataaaa gaggtaatgt agttggatat
    11101 tctaagtttg agacattccc agtttggaat gtacctgtaa aacatccttt gaatatagct
    11161 tatgaagcag ctacagttga tttaaaagat gtgaatatga tagattcatt ccaccttgaa
    11221 acgtatggtg aaatgtcagt aaactacaat agagatttgg aattatttcc agtgcttaaa
    11281 aaaataatag aaaagataac tggaaaagaa tcaattttcc aatctccaac tgatatgggt
    11341 gttaatagag ttggctttgg aataatagat gatgaagtaa taagaaaagc ttctatagaa
    11401 gaaataataa gaagatactt taaaactgcc tgtgagtata aaaagggaca agttgataaa
    11461 ggtgcttatg atagaatgaa aatgattatg gaagagctta acttaaagcc agaagataga
    11521 aatgttgtat tacctgctag aaactacagt aataaattga aagaaagtgc cgacaagaat
    11581 gatacttgtc ctgtagttgc attagaatta gaagatggta caatattaac tggtaagggt
    11641 tctgatttaa tgaatggaac tgcagcagta gtattaaatg ccattaaaca tttagccaat
    11701 atatctgatg atatgcactt aatatcacct gtaatattag aacctatcat aaacttaaaa
    11761 acaaagactt tagctagtag aaatgtttca cttagttgcc aagaggtgtt aactgcactt
    11821 agtatttgtg ctgtaactaa tccaactgca caatttgcta tggaaaagtt agcattatta
    11881 aaagggtgtc aagctcattc tactactata ttaaatagag atgatgaaca aacatttaga
    11941 aagcttggta tagatgtaac ttgtgaccca gaatatcctt cacaaaatct ttactatagt
    12001 taaaattata taaaaaaata tgggctgttt taaagtaata gtttgagcag tccattacaa
    12061 ttataataaa atcagtaggc tgtcttgtat aagacagtct tttgttttta aaacatccca
    12121 taaattaaat atatatactg ttatccataa ggaattttaa atttagttcc ttggaatacc
    12181 tattgtaaag cttttatata gtttttgcaa gtgtttttaa ggatttttta taaattatat
    12241 tgaagttaat ggattataga tggttttatt tatggaaaat atgaatatat aaagaaaata
    12301 atgaaaaaat accaaaaaga aacataagtt tggtataatt aaaaagtaat ataaataaaa
    12361 ttaatctatt tatactttaa aaggtgataa taatgaaaca ttatagaaaa ataattcata
    12421 tagatatgga cgctttttat gcatctatag aacaaagaga taataaaaaa ttaaaaggaa
    12481 gacctgttat tgtcggtgga aatccacaaa gccgaggtgt ggttgcaacg tgttcttatg
    12541 aagctagaaa atttggtatc cattcagcta tgccatcagc tgtagcttac aataggtgtc
    12601 catatgcagt ttttgtaaga cctcgatttg atgtttataa gagtgtgtca gagcaaataa
    12661 gagatatatt ttatagatac acggatttag ttgaaccatt atctttagat gaagcttact
    12721 tagatgtaac aaaaaataaa aaaaatattg attcatcaat tgagatagca aagcaaataa
    12781 aaaaagatat atttagagaa gttggtctta cttcttcagc tggtgtgtca tacaataagt
    12841 ttcttgctaa gattgcatca gacttaagaa agccaaatgg gttaactgta ataaccgaag
    12901 aaaatgccca ggacttttta gataaattac ctgtgaataa attttttggt gttggaaaag
    12961 ttacttcaaa tactcttaaa aatttaggta taaaaactgg atatgattta aggtgtttaa
    13021 atttatttga acttgaaaat atattcaaaa aaagaggtta tgaattatat aaatttgcta
    13081 gaggaataga cgatagacca gtggaaccaa atagggttag aaaatctgtt ggggctgaga
    13141 ctacattaag tcataatttg gatattgatg aagaagaaac acgcaacatt cttgatgaac
    13201 tttgtgaaga agtatgtcat agattaaaaa actctgagaa gtttggaaaa acattaacat
    13261 taaaaattaa atatgaggac tttacaaaga taactagaag ccttagttta gaacactata
    13321 ttgacgaata taatgatata agaagtggtg tagataattt gttgagaaat gttgatgtta
    13381 atggaaaaca aattaggcta ttgggagtta caatatcaaa cctaagtgat aaaaaagaaa
    13441 cttataaaga tattacatta tttgaataca tggatagtat ccaaatgtga gttttgttta
    13501 aatgaaataa cactagcttt aatactatat aattgaggct agtgttattt tatgaatatt
    13561 tttatatttt ttgttttttg acattatata ttatatacta tatataaata ctttatattt
    13621 gtagcaatat tcagacaaat agtgacctaa aaatcgtact ttgtttacat aaataaaata
    13681 gtatgatata attataaaaa caacttataa tattgaagaa ggttgcttgt tcgaaaaagg
    13741 ggatgataaa ttatgataga gataagaaat gtaacaaaaa aaataggaaa taatgttata
    13801 ttagatgata tttctctagt tgtagaaaca ggtacattgg tagttttgat tggttcaagt
    13861 ggttgtggaa agactactac tttgaagctt ataaacaaat taataaaacc tacatctggt
    13921 gagatatata ttaatggcaa accaatttct caggaaaatg aaatagagct tagaagaaaa
    13981 ataggatatg ttatacagaa cacaggccta ttccctcatc ttacaataaa agaaaatata
    14041 gagctaattc cacgcctaaa aaaggaaaaa tccgttgaag aaattgaaaa aagaacgttg
    14101 caactacttg agatggtagg acttgatagt gatgaatttt tgaataagta tccttcagaa
    14161 cttagtggtg gacagcaaca aagaatcggt gttgcaagag ccattgctac tgatgctgaa
    14221 ataatattga tggatgagcc ttttagtgct ttagacccaa taacgagaac atctttacaa
    14281 gaacaattat tttctcttca agatgaattg aaaaaaacta taatatttgt aactcatgat
    14341 atggatgagg ctttaaagat agctgataaa atttgtatta tgaaggatgg aagaatagca
    14401 caatatgata ctcccgaaaa tatacttaga aagcctgcaa atgactttgt aaaagatttt
    14461 atcggagaag atagagtttg ggataatcca gaatatataa aagcaagaga tattatgata
    14521 aaaaatccta ttgctgttaa ttctactaga actgtaactc aaggaataga aattatgaga
    14581 acgagtaagg ttgatagctt actaattata gatagagcaa aaacattaaa aggtattgtt
    14641 actgtaaaag atatgaaaga tattgatgat aagtcaatat tacttgctga tattatgtca
    14701 agtgagcctt tgcatgtaaa tgaaggtgat aatttagtag aaatactaaa tgtaatgaat
    14761 cgtaattcgg ttggatatat tccggttatt tctgatgaaa ataaactggt agggcttatt
    14821 actagaagta gtttactttc tgttttaagt gaacaattct tagaaatgga ggtgagtgtt
    14881 cttggctaat ttttttaact ttatactatt acaaaaagat aagattatag agcttttaat
    14941 acaacatatg agtctaactg ttacatctat tttaatagct ataatagttg gtgttcctct
    15001 tggaataatt atttcaagaa tctcatctct tagaaagttt gtacttggat ttgtaaactt
    15061 agtccaagca gttccgtcta tggcactttt gggtcttctt gtacctatac ttggtatagg
    15121 aagtaaacca gccatattta tggtagtggt ttattcatta ctaccaattg taaaaaatac
    15181 gtatataggt ataacaagta ttgacccagt agtattagaa tcagctaaag gtattggtct
    15241 tacaagaaat cagacattat ttaaaataca attcccttta gcacttccta taataatggg
    15301 tggagttaga atatctgcag ttacagctgt tggtttaatg actcttgcag catttatagg
    15361 tgctggtggt ttaggatatt tagtattttc aggggttcaa actgtaaata ataatatgat
    15421 tttagcaggg gcaatcccat catgtataat tgcactgtta gttgattatt tatttagtaa
    15481 aattgaagtt gcagttactc caaaaggatt aaatccaaaa gcacctaaga agaattatat
    15541 agcacttaaa gtaatatctg tagtggttgt tgtttcaatg ttgttcgttg tttttagttc
    15601 tagtttttca agtaaaaaag atacaataac tattggttct aaagattata cagaacaact
    15661 tatacttggt aatgtttatg ctgaattagt agaaaaaaat acgaatctta aagttaagaa
    15721 gaatttaaat ctaggtggtt catctgttgc gtttaatgca attaaatctg gtgagttaga
    15781 catgtatgtt gactatacag gtacactttt ggtaaatgta atgaaacatg ctccgattaa
    15841 agatgctgat gaagcatata atgttgtaaa agatactatg gaaaaagaaa atcagttaac
    15901 tttacttgac cctcttggat ttaataatac ttacacatta gctatgatgc ctgaaactgc
    15961 tgaaaagtat ggaataaata ctatttctga tttgactaaa tatggtaaag aatttacatt
    16021 ctcaccaact ttggagtttg aaaatagaga agatggtctt gtagggcttt ctagagatta
    16081 tggtttaaaa ttcaaagatg taaaagcaat gaatggaagt ttaagatata cagctttaga
    16141 taataatgaa tctcaggtta tagatgcatt tttaactgat ggtcttatta aaaagtttaa
    16201 tcttaagata ttagaggacg ataaaaattt ctttgtacca tattatgcag caccattagt
    16261 aagagaagat actttgaaga aatatcctga gttagaaaaa gttatgaata tgctttcagg
    16321 aaaaataaat gaagaaacta tgagagaact taactatcaa gtggatgaac ttggtaaatc
    16381 accagaagaa gttgctcatt cattcttagt taaagaaggc ttagtataaa aaattaatag
    16441 tttatatcaa aaatttaagg gagtgtctca aaatgaactt tttagttcat gaggcactct
    16501 tttttatatg aaatcaaata tattttatca tttcaacaat aaaaaagaag attatcttta
    16561 ttttgagaca gtctcttatt tttatgtata ccaaaaccat acctacgatt tttatttata
    16621 attaagtttc aaatagaatg aacaagtaca accaattttg aatataaata tagtaaatgt
    16681 gcttatatac gattttattg tctggttgat tgatattgat agatagcttt ctttattgaa
    16741 tcgaagggag ggggaatttg ttatggataa ctataagaaa gtgaagcaag tattgtggat
    16801 aattttattt gctaattttg cagtagcatt attaaaaatc attattggta atcaaataaa
    16861 aagctatagt atgacagcag acggatttca ttctttgtca gatggagcat ctaatatagt
    16921 ggggcttata ggtattttct ttgcatctaa accaaaagat aaaaatcatc cttatggaca
    16981 taaaaagttt gaaattataa ccagtttatt tataagtggt atgttgtttg ttatagcaat
    17041 taagataatt cttagtgcag tgttaagaat agctaatcca gttgtacctg ctataactat
    17101 agaaagttta attgctttaa ttattacact ttttataaat atttttgtat gcatgtatga
    17161 atatagaatt gggactaaac taaatagtta tgttttgatt tcggattcat tacatacaag
    17221 aagtgatata tttgtgtctc taggtgtatt ggttacttta gttggtgtta agttaggatt
    17281 tccagtaata atagaatcta tagttcctat tattatttct gcattcataa tttattcggc
    17341 ttatggaata tttaggccat ctattggaat tttggtcgat agagtggctg tagatgagga
    17401 ttatataaaa gaaattgtgt ttgaatttaa tgaggtaagg gatgttcaca atatcagaag
    17461 tagaggtagc aaaagttcta tttatattga catgcatgta atggtagacc catttataag
    17521 cgtagagcaa tctcatgatt taacacataa aattgagaaa caaatacaag aagaaattaa
    17581 tgaaaatgct caagtaatag tccatattga acctttttat agtttttaac ttagttataa
    17641 aatacaagtt ataaaaaaat ataatttata aaaaaaatat tataccataa ataatttata
    17701 aaaattattt atggtataat attttctata aagaaattaa gtcttaatat atctaaggag
    17761 gaatttacaa tgagatacgt tgaatatgga aagacaggga aaatggtttc agtagttggc
    17821 tatggaggat taaggtttga tttagaaaaa agtaatcaag aaaatgctga tttagtcaaa
    17881 tatgcgtatg aaaaaggtat taattacttt gatacagcac cagggtattg tgatgacaga
    17941 agtgaagata tatttggact agcctttaga caaatgataa aggaaggtaa gaccgatttt
    18001 tatgtatcaa ctaaagaaaa gccaaaagca tgtgatacat cagaaaaagc tatagaggct
    18061 gtaaaaaaat caatagaacg attaggcgta tctaaaataa atttttatca tgtttggaat
    18121 attagaaaga tggaacatta cgaactagca atgagacctg gaggtcaata tgaaggatta
    18181 ttgaaatgta aagaagaagg attgatagac catatagtat tttcatctca tcaaccagga
    18241 gatgaggtta tagatattct aagtgaaaat aagcttgaag gtgtaactat gggaataaat
    18301 atattaaact ttccatatag gttaaaaggt gccaaatatg ctgttgataa tggatatggt
    18361 gttgtggcaa tgaatccatt aagtggtgga actattccaa agtataataa agaactttct
    18421 tttctatcaa aagatggcga aagtactata gaatcagctt taagattcaa cataggaatt
    18481 tcacaaataa gtatatcttt gatagggttt aataaaaaac aagatataga tgatgcatgt
    18541 aaaattgcag acgaaaatag aatttattca gatgaggata tacatgatat tgagactaga
    18601 ttaaataaga atatgaacga aatatgtaca ggttgtggtt attgtaaagt atgtccaaaa
    18661 ggtattaata ccccagccta tatgcttttt tataatgaaa agcagatgtt taaaaagagt
    18721 gatgaagaaa tgacaaagtt agtttatggc ttaggacatt ggaattacac aatgaatagt
    18781 aaggcaaaag ctaaagaatg tatatcttgt ggaaaatgtg aagttgaatg tactcagcat
    18841 ttacctataa tagatagatt aaaagaaatt aaaaaatggg aagaagatgg agctaatagt
    18901 gttaaagtct aattaatttt atactatatt tgaaatttgt atagtattta gtaatgtttt
    18961 aaaatatagt tttaatattt aaggagacag attataattt tgtctcctta aattatgctt
    19021 taatttgtta tagtttaatt ccataagtaa cattgtatat ttttatataa agtttttcaa
    19081 atattttaca ttaatattgc ttacaaattc tcattttata caacactaat ctatgttata
    19141 taaacaatac ttgactgatt ataacatcaa taaagtaaat attgtggttg taaataaaga
    19201 gttattcaat tacaataaaa caagtaagaa cattatgaag atggctatta aatataagga
    19261 ataaattgta ggtgtcagca aatcctatat aatgaatgga tggtgatata ttgatgagta
    19321 aaatattaaa atataaaaac gagatgtttt tgtttatatt agttgtaata acttacttaa
    19381 taataactaa gatatttttt tctaagacta ctatatttta tgatttaaac aatacttatg
    19441 atgtattgtt agatacagat acaggagttt tattcaattt aaatgtattt gctataagtc
    19501 aagataatag taagcatata ttgttttcag caattatatc aatttttgca tatccaatat
    19561 acctattttg tacttcaata gcaaatcctg ggacaacaga ttttaatagt gcctatgggt
    19621 ttggactaat ttgtctacaa ataataacta gtgctatgtc aataacttta gtctttaatc
    19681 atattaaaaa gataaaaatg cagagattaa ctttaatttt acttactatg ataatgatat
    19741 tttcttttcc acagttattt atgactttaa atgtagagag atttatatat agccaatttt
    19801 cattaatatt tttcatagtt attgcaaata agatgaaagg aaaaaattct tatttagtag
    19861 aattagcagc tattcctcta tttggaataa ctattagtaa tatatatttg tattttttta
    19921 atatgatatt tgagtttaaa ttaaaaatag gaaaaatgtt aaaacattta attacattta
    19981 tcctaatggc atatatttgt gtagtttcaa cgaagtctta tgagagtttt atgaaccttg
    20041 gaaatgtaat acagtatgat acgaaattta taagtggaga accaattttg gaaaagattg
    20101 caatgattat agagagatta ttgtattcgg ttttttattt tcctggagca agtattaaaa
    20161 aagggttatt tttgcaaaat ggagaagttg ctacaatacc agtaattctt acattattag
    20221 ctttatgttt ttgtgtttta tcagttattg aaaattcaga aaaaagagtt cctaagctat
    20281 gtatgggaat aattatattc aatctaactt tacatggtat agtaggatat aatctagtca
    20341 attcaagtat tatggctata aattttagtt ttgcagttat aattttattg gcttacttta
    20401 cgaaagcgct tagaaaaaat gaaaagaata tgtataatat atttctatcg ctgttattgg
    20461 ttactatagt aatcagtaat gtcaatggat ttatagaaat tttaaacata gggataaaat
    20521 catatcctgt ttaaaaaata atattgaaag ggtagtaagt ttatgaaaca gaccaataca
    20581 agtataaggc ttcttactaa aatttcagat tatataaagt taatgagaat aaagcaatat
    20641 ataaagaatt tctttgtatt ttcagctata attttttcaa acaacatact aaatataaat
    20701 ttatttttaa atacgtttat tgcgtttgtg tgtttttgtc taatgtcatc atcagtatat
    20761 gcactgaatg atgctataga catggataaa gataaaaaac atcctaaaaa atgtaataga
    20821 ccagtggcaa gtggaagaat aagcaaaaaa agtgctaata tactatttgt agtattagct
    20881 ttaatttcag tctgtttatc aacagttgtt agttttaatc tttctataat tctcagtctc
    20941 tatttgataa acaatatatt atattctctt aaactaaaga atataatact attagatgtg
    21001 ttttcaatat ctcttggttt tatacttagg gtttatgcag gatgtgtagc cataagtgtt
    21061 agtttatcaa attggataat tttatgtaca tttttcttat ctctgtactt agctcttgga
    21121 aaacgtaaaa aagaaataga aactctaaga gatgatgcag ttgaacatag aaaaatattg
    21181 gaagattatg acatagaaaa tctaaatcaa atgatgatag ttatattaag ttctacaatt
    21241 gtatgttatg cattgtattc tacaagtaat ccagaaaagc cacatatgat atttacaaca
    21301 atatttgtag tttatggtgt acttagatat aattatataa ttaacactac aaatgaaaat
    21361 aatcctactg atattgtcct aaaagataat gctttaaaaa taaacgtaat cttatggata
    21421 attacatgtt taataatatt gattttttag tgatattttt agtgatattt ttagtgatag
    21481 gatggggaaa tacttatgaa aaaaagtgta ttattacttg atgtagatta tactgttata
    21541 aatactgata gcatgataga tttttttatt tactctttaa aaaataaaac ctttaaaact
    21601 attataaagt tgccatacat tatttttatt ctatttatgt atttaattag aatgataccc
    21661 ttgaagaagg caaaagaggc aatcttttat ccaatagtag attttagtga agaagattta
    21721 aaaaagtttt ttgatgattg cataatgaaa aaaattaacg aatctatgaa aaaggttatt
    21781 tacaaaaata aagaagaaga taatgttata attatgataa ctgcatctcc atatgcatat
    21841 atgaaatatt ttaagtatta tggtcttgca gatgaggtta taggcacgga gtttttttat
    21901 gaaaattcaa gatataaaaa tcaattcatt ggagaaaatt gtaagggaat tgaaaaatta
    21961 aaaagaataa aggctgtatt aggtaaactt gggatagaga ttgattatga aaattcatat
    22021 gcatattcag actctaagag tgatttacca atgctttcat tgactaaaaa tgcattttta
    22081 gttagtaaaa aggatggtga ggttatagag caaataaatt cataacttaa tgtcacttag
    22141 aataggaggg aatagtttgg gatatttaca catattaatt ttatctatgg ttcctgtaac
    22201 agaattgagg ggagccattc ctataggtat tgcaatgggc ttaaatccta tatgggttta
    22261 tgtatttagt gtaataggtt ctacattggt atcgattccc ttgatattga cattcagaca
    22321 tgtattacaa tttttaagag ggaagaaact atttaaaggg attgctgatg taattgatag
    22381 aaaaataaac tcaagaatga agaaattaaa aagtgtttcg ataattggta taatactatt
    22441 tgtaggtata ccactgccaa ctacaggaac atggactgca tctgctatag cttccatatt
    22501 aaaaatgaga ataaaagatg cttttatggg agtatttctt ggcaatttgt tatcaggggt
    22561 tatagtttca gctctttctt tacatataat ataaaactat ctaagtaata tacttggata
    22621 gtttttttat gagtatgtgt gaaaaatttt taataataaa taaaaaatga gataaagtat
    22681 ttaacttcag ttagttctaa aatgtttact atggcaaaag aaatatagta tagtattact
    22741 tgtagggaaa gtactctaaa tttgtctgct aataaaatga aatttttata tagaataaaa
    22801 ttttagtgat acattttaat tattggattg aggaggtttt aaaatggaaa attcaaacac
    22861 taacttaggt agtgaaagtg tgggtaaatt attatttaaa cttgctacac ctgctattat
    22921 cgcacaaatt gtaaatgttt tatacaacat agtcgatagg atttttattg gtagaatgga
    22981 aaatggagaa gttgctatgg ctggtgttgg agtggcattt ccaataataa ttataataac
    23041 tgcatgtagt tatttaatcg gtatgggagg aggtccatta gctgctataa aaatgggtga
    23101 gcaaaacaat gatgaggcag agaaaataat gagtaacagt ttttctgtac tggtgatttt
    23161 agcaatactt ttgactatag gttttaagat tggaaaagag cctttacttt ggatgtttgg
    23221 agcaagtgaa tctacaattg gatattcaat ggactattta aatatttatt taataggtac
    23281 agtgtttgtt cagatttcaa tgggtatgaa tacttttata aatacccaag ggtttgctac
    23341 aactggaatg atgacagttg ctataggtgc tttaataaac ataatacttg acccgatttt
    23401 tatatttggc tttaatatgg gtgtaaaagg agctgcatta gctactataa ttgcacaagg
    23461 cgtttctgct atttgggtac ttatgtttct ctttggaaag aaaagtatac ttaaaataaa
    23521 aaagaagtat atgataccaa aagcaagtat catcttacca gtgcttggtt tgggaatctc
    23581 tccatttata atgcaatcta cagaaagttt agtacttata gccttaaata gtaaattaca
    23641 aatgtatgga ggagatttag cagtaggttc aatggctata atgagcagta taatgcaaat
    23701 tcttatgctt cctaatatgg gggttacaca aggagcacaa ccaataataa gttataatta
    23761 tggttcaggt caacttgata gagtaaaaaa gacatttaag ttgtgtttgt tgtcatgttt
    23821 tacatattct actattttat ggcttttatt aatgatattt cctgcatttt ttgttagtat
    23881 atttaataag aatccgcagt tacttagtat gactagttgg agtataaaaa tatattttgc
    23941 tggtgctttt atgtttggga ttcaaatagc ttgtcaacag acatttttag cacttggaaa
    24001 agcaacaata tctttggttt tagctctact tagaaagatt gtacttttaa tacctttaat
    24061 atttatactc ccaacattct ttaatgaaaa actttttgca gtaattttag cagagccagt
    24121 tgcagatata acagctgcta ctataacagc aatttctttc tttatatttt ataaatgttt
    24181 tctatcaaaa cctaaagcaa taaaagaata agatatacaa tataaatttt ttatgaaaat
    24241 attttattag ataaaaatta gaatttgtat tttaatttaa taagatattt tctataatgt
    24301 ataaatatat gaagttgaat ttattttaaa aattatagta agaaaaatgt aagtatttgc
    24361 aatattaatg gtataatatt tacagatatg ataatcaata agaggaaagg atgtttataa
    24421 tggaaaatga tttaacaatg caagaattac ttgaccagca agaacaagta ttcagcaaag
    24481 tgaaagttgg agaattgaca actggaaaaa taactgcagt gagaaatgat gaagtacaat
    24541 taggattaga ctatgggttt gatggaataa tacctataag tgaattgaac atagaaaaaa
    24601 atcaatatat agaagatata taccacatag gtgatgaaat aactgctgta ataacaaaag
    24661 tatctcaaaa agatggtaca ataactttat ctaaattaca acttgacaaa agaaatgatt
    24721 ttgccgagtt acaaaaggct tatgatgaac atagaataat aactgtaaat gttgaaaaga
    24781 atatagataa aggtgtattt gctaattaca atacatatac attttttata ccaatttctc
    24841 aacttgatac aaaatttatt acagatacat ctaaatttgt tggtcttaac ttagaagttt
    24901 atataaaaga attggatgtt agaaaaaata gacttgtagc atctcataga gatgtattac
    24961 aagagcgtat aaataaagag agagaagaaa gaagagctca aattaaggca gaaaaagaag
    25021 ctgaaagagc aagaattaag caagaaagag aagaagaaaa agctcgtatc aaagcagcta
    25081 aagaagactt atttaattct cttgaagttg gacaaaaaag agatggtaag gtgacaaaga
    25141 taatgcctta tggtgcattt gtggatattg gtgggattga aggattagca catataaata
    25201 atttagcttg gacaagagtt gaaagtgttg aagatgttgt ttcagaggga caagaagtag
    25261 aagtttatgt gttggatgta aataaagaga ctaaaaaaat tgccttggca ttaaaagaca
    25321 taaataatga cccttgggat ttaatagcaa aagaagtaca aattgatgat gttgtcaatg
    25381 ctaaagttct tagaataata gaaaaagggg cttttgttca aattaaagaa ggtgtagatg
    25441 cgtatttacc aatatctgaa ttgagtgata ctagagtggc taaagttact aatgttgtta
    25501 atattggaga tgaggttaaa gttaagattc ttgattttaa accaaagact aaaagaatgt
    25561 tggtttcaat aaaagaagca actagagagc cagaagagga tataacagaa tacctagaag
    25621 tagaggaatc tttaggaagt attggagaat tatttaaaga taaatttaaa gatttagaag
    25681 tgtaaaataa ttgaaaatgg ggctgtttta aaacgttaca ctgtctatta tgacagcttt
    25741 agttttgaaa tagcccttta gctattttca cgaatttatg taaaatatta ggaggaaatt
    25801 taatgaaaaa gtctattatg gatgtacatt gtcacactct aataagtgga catgcacata
    25861 gtactttcaa ggaaaatgtc gaagaggcga gtaagaagaa tataaaatac ttaggaatat
    25921 cagaccatgg acctaatatg ccaggaggac cacatccatt ctatttttat aatttgcatt
    25981 tgcttccacg tgaagtgcaa ggcgtaaaga ttcttagagg aatagaagga aatataatgg
    26041 attataatgg taatttagat gttcaagaag atatgttgca acatttggat tatataatcg
    26101 caagtttgca tagaccttgt atagcttctg gtacaaaaga agaaaataca aatgctattt
    26161 taaaagttat ggataaacct aaggtaaaaa taattggaca tccagatgac agtaggtatc
    26221 ctttagatta tgaacctata gtaaaaaaag ctaaagataa aaatatcctt ttagaaataa
    26281 ataattcatc attaagttca aactctcata gaacgggtac ttgggaaaat gtaagtcata
    26341 tgttgacatt gtgtaaaaca tatggtgcaa gagtaattct tggtactgac tctcatatat
    26401 gttattctat aggagaattt gaaaatgcag aaaaagtttt agaagcagta gattttccag
    26461 atgaattagt cataaattat catgaagatg aaattataga attttttgat ataaattttt
    26521 agaatcttgg gagtgaaaaa atttgagtaa aaataagttt atctatatga attattatga
    26581 caatggttat tttaaaaaag gaatgactat atatatggtt ttagcactta taattttagc
    26641 tacaattttt atatattcaa cgacaatatt atatatttta tgtataataa cagcagtttt
    26701 tctatttaaa
//
LOCUS       DAGSHT010000033        25818 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGSHT010000033 DAGSHT010000000
VERSION     DAGSHT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.034"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1046)
                     /locus_tag="MJB72_001274"
     CDS             complement(<1..1046)
                     /locus_tag="MJB72_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY8262399.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNN"
     gene            complement(1139..2011)
                     /locus_tag="MJB72_001275"
     CDS             complement(1139..2011)
                     /locus_tag="MJB72_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBY8262400.1"
                     /translation="MRVNTNVSALVANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSLQSSNETN
                     TAEERQKVADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNVDGASGTDAAKQMVSSLDMALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(2113..2448)
                     /gene="fliT"
                     /locus_tag="MJB72_001276"
     CDS             complement(2113..2448)
                     /gene="fliT"
                     /locus_tag="MJB72_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBY8262401.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYINISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            complement(2451..3974)
                     /gene="fliD"
                     /locus_tag="MJB72_001277"
     CDS             complement(2451..3974)
                     /gene="fliD"
                     /locus_tag="MJB72_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBY8262402.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKFANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKSKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(3995..4351)
                     /gene="fliS"
                     /locus_tag="MJB72_001278"
     CDS             complement(3995..4351)
                     /gene="fliS"
                     /locus_tag="MJB72_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBY8262403.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            complement(4351..4746)
                     /gene="fliS"
                     /locus_tag="MJB72_001279"
     CDS             complement(4351..4746)
                     /gene="fliS"
                     /locus_tag="MJB72_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBY8262404.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            complement(4768..4980)
                     /gene="csrA"
                     /locus_tag="MJB72_001280"
     CDS             complement(4768..4980)
                     /gene="csrA"
                     /locus_tag="MJB72_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBY8262405.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            complement(4974..5366)
                     /locus_tag="MJB72_001281"
     CDS             complement(4974..5366)
                     /locus_tag="MJB72_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBY8262406.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            complement(5382..6314)
                     /gene="flgL"
                     /locus_tag="MJB72_001282"
     CDS             complement(5382..6314)
                     /gene="flgL"
                     /locus_tag="MJB72_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBY8262407.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(6333..7643)
                     /gene="flgK"
                     /locus_tag="MJB72_001283"
     CDS             complement(6333..7643)
                     /gene="flgK"
                     /locus_tag="MJB72_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBY8262408.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(7661..8077)
                     /locus_tag="MJB72_001284"
     CDS             complement(7661..8077)
                     /locus_tag="MJB72_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBY8262409.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            complement(8082..8357)
                     /gene="flgM"
                     /locus_tag="MJB72_001285"
     CDS             complement(8082..8357)
                     /gene="flgM"
                     /locus_tag="MJB72_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBY8262410.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(8509..8823)
                     /locus_tag="MJB72_001286"
     CDS             complement(8509..8823)
                     /locus_tag="MJB72_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY8262411.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            complement(8847..9275)
                     /locus_tag="MJB72_001287"
     CDS             complement(8847..9275)
                     /locus_tag="MJB72_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBY8262412.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(9284..9952)
                     /locus_tag="MJB72_001288"
     CDS             complement(9284..9952)
                     /locus_tag="MJB72_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBY8262413.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(9982..10965)
                     /gene="rfbB"
                     /locus_tag="MJB72_001289"
     CDS             complement(9982..10965)
                     /gene="rfbB"
                     /locus_tag="MJB72_001289"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBY8262414.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            complement(11003..11560)
                     /gene="rfbC"
                     /locus_tag="MJB72_001290"
     CDS             complement(11003..11560)
                     /gene="rfbC"
                     /locus_tag="MJB72_001290"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBY8262415.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            complement(11609..12478)
                     /gene="rfbA"
                     /locus_tag="MJB72_001291"
     CDS             complement(11609..12478)
                     /gene="rfbA"
                     /locus_tag="MJB72_001291"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBY8262416.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            complement(12587..13459)
                     /gene="rfbD"
                     /locus_tag="MJB72_001292"
     CDS             complement(12587..13459)
                     /gene="rfbD"
                     /locus_tag="MJB72_001292"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBY8262417.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            complement(13665..18746)
                     /locus_tag="MJB72_001293"
                     /pseudo
     CDS             complement(13665..18746)
                     /locus_tag="MJB72_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(18757..20289)
                     /gene="purH"
                     /locus_tag="MJB72_001294"
     CDS             complement(18757..20289)
                     /gene="purH"
                     /locus_tag="MJB72_001294"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBY8262418.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(20282..20875)
                     /locus_tag="MJB72_001295"
     CDS             complement(20282..20875)
                     /locus_tag="MJB72_001295"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBY8262419.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(20869..21945)
                     /locus_tag="MJB72_001296"
     CDS             complement(20869..21945)
                     /locus_tag="MJB72_001296"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBY8262420.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(21939..23306)
                     /gene="purF"
                     /locus_tag="MJB72_001297"
     CDS             complement(21939..23306)
                     /gene="purF"
                     /locus_tag="MJB72_001297"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBY8262421.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(23318..24016)
                     /locus_tag="MJB72_001298"
     CDS             complement(23318..24016)
                     /locus_tag="MJB72_001298"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBY8262422.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(24022..24498)
                     /gene="purE"
                     /locus_tag="MJB72_001299"
     CDS             complement(24022..24498)
                     /gene="purE"
                     /locus_tag="MJB72_001299"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBY8262423.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            24948..25460
                     /locus_tag="MJB72_001300"
     CDS             24948..25460
                     /locus_tag="MJB72_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY8262424.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
BASE COUNT         7685 a         4804 c         2760 g        10569 t
ORIGIN      
        1 atgttattgt actctgaaat tacaaactca tatttttcaa gcttataata aatctttatc
       61 agtgtaataa tagcctcatc tctaagccct acagtatcag tgtctgaaaa taaactattt
      121 gcttgtgtac ttatttcata attatctaac aagtaaatat atctcttata cgaatctata
      181 ctattttcat actttcctaa atctacttgt gcttgtgcaa tataataata tatatcaata
      241 ttattcatgt ctttcttaat atactttaag cacaattttt cacacttctt atgttttcct
      301 aaatataaat atgtttttac aaggcttgtt atcaagtaac caggtgccga ttctagttta
      361 gaatataatt cataagattt ctcaagatag tataaagcct cctgatattt tcctaatata
      421 ataaaatttt tccccaattg atagtttgta tatggagaat tatcattctc ttttaactct
      481 tgaaataata gtttttcatt tcttttcacc ttatttaatc ttatttcttc atcttcaaac
      541 aaatatccat aatgttcaaa atcagcaaca ttattaaata taggattttt gtatattggt
      601 tgttcatgta ctttaccatg gtatctaaaa tcaacgtttt taaaaagtct aagcaccgaa
      661 caatatccat ataatttttt atcgtatgag taaagatttt ttaattgtat tgaagcagaa
      721 ttaaattttt tatcaagacc gtccttgaaa aattttacaa tagttgaaga atcaattaat
      781 atttcatctg catctaatat aagtaaccat tcgcccttgg cataagatat tgatatattt
      841 ctcatactag aaaaatcatt attccaatta tgaaagtaca cattttctgt gtatttccta
      901 gatatattta tagtatcatc tgtagaacct gtatcgacta ttataagttc actttctata
      961 gaatttctta atgtggttaa tgattttaaa gttttttcaa gtattttttc ttcattttta
     1021 acaatcataa ctatactaag taacatataa attttccttt caaaagtata tttgtaaaaa
     1081 aagaaaggag taaagcaaag ccttaccctt tcttctatat ccaatataca aaattatttt
     1141 atcctaataa ttgtaaaact ccttgtggtt gttgattagc ttgagcaagc attgactgtg
     1201 atgcttgaac taatatattc attttagata agttaaccat ttctgaagca acatctgtat
     1261 ctcttattct tgattcagct gctgtaacat tttctatagt attatttaag ttgttttgag
     1321 ttgattctag tctattttgt tgtgccccta attttgctct tgatgtattt aattctttaa
     1381 gagccatatc taagctagac accatttgtt tagctgcatc tgttccagaa gctccatcta
     1441 cattcaagct tgctattgta gaacttgtta tacctgcttt actcattata cttgaagtat
     1501 ttactagagc aacttttatt tcattgttgt tgttagttgt tcctgcaaca tttgttccaa
     1561 agtttgctcc aacttgtaat cttatttctg tagaacttcc atcaagtaat ttcttaccat
     1621 taaactcaat tgagcttgat attctttcaa cttcatcttt taattgtaat aactcatcag
     1681 ctaccttttg tctttcttca gcagtattag tttcatttga agattgtaat gataaagttc
     1741 tcattctttg tagtatgtta ccagtttctt ctaatgcacc ttctgctgtt tgtacaacag
     1801 atataccatc ttgaacgttt cttcctgctt ggtctaaacc ttttatttga gctctcattt
     1861 tctcagatat agcaagccca gcagcatcgt cagcagctct cttaattcta acaccagaag
     1921 atagcttctc catagactta ctttgtgcat ttacgtttct tcccatttgg ttatttgcta
     1981 ccaaagcact tacatttgta ttaactctca ttattttacc ctccttagta tagttgacat
     2041 ccttgtcatt tctactttta atctttttta ttaattcttc atttatataa tcggacaaga
     2101 tggtacataa ctttatactt ttttattaaa aatatttact ttttctctat ttaagttata
     2161 atacattgtg ttcacttgtt tagtaagctt atactcatgt aattcttttt ttacttctaa
     2221 taattgtcct tgtaaaatat ctcttatcct actatcaatt tcaattaatt ccattctatt
     2281 ataaagctgt ataaaatcat ttctatcaat ttcacttaca ctatttataa tctcttgtct
     2341 ttcacctaac ttactactta tatttatata ttcttccttt tcaatcaaat ttataatttg
     2401 taaagatata tctttatata aatttaattt ttcttttaat ttatccataa ttaattaccc
     2461 tgtgcttgtg agaaataatt catttgggaa gaatactgat tcaacaaaga ttctaaggct
     2521 gaatatttag cataaagtct tttctcttta tcattcattt tttcaactaa cctgcttata
     2581 tttctttctt gctctgcaat ttgttttgaa tatacatttt cactagcaga agcagttttt
     2641 tctatacctg cttttttagg aagtttagaa gttgaacttc cagaatattc atatacaata
     2701 tcttttagct tttcaaacat tcccttatca tcataattcg aagaataacc tgctagtgtt
     2761 ttgtatacct tatcactatc atctataaga gcctttgaaa aagtatctgc attaaatgat
     2821 atttgacctt ttttattgta attttcgctt tcattgatac ctagttttct taatgcaata
     2881 atattttttc catctccaaa aaatgcagac tgaatatctt caacaaaagc tcttaactca
     2941 tcatcatttc taagtatacc ttctttagct ttcttttccc atttttctat ttcttcagtt
     3001 gtcatatctt ctttttgggc atctgtaaga ggtggataat cttttgattt tttagtagta
     3061 actaaaccat agaccttgtc catcagttta ttataatcat ccacaaatgc ttgcatattt
     3121 ttaacagctt cttcagtatc agttacagaa gtcaactttg cagttccttt tgaatttaca
     3181 ttatagtcaa tattatctat attaaatgaa ttttgttctt tacttatatg agttactctt
     3241 ccatgttcat cagttacatc agcttctaga ttctttccat atacatttgc aaatttgttt
     3301 ccaccagcat catttataaa tgtgatacca ttatcattag caatttgctg agctaaattg
     3361 tcatttccaa ttactcttaa attaatgtct gatgacgaac cagtctgttt tcttgaaata
     3421 atcaattcac ctgtcatctc actataagaa gcctttatat cattatctgc gcaaagggag
     3481 tttatcttac ttacaatagt acttgtagta tcttgttcag atattggtat atttacatct
     3541 cctatagtta aagaactagc tccactggca tttggaggga actgtcgaac aataggttca
     3601 cttgaattta atttaatagt aaattttgct ggttcagcca tttgagaaac tgcaaattga
     3661 taatttattt tttctgcacc agctgaacct tttgctacta taatactttc atcagaactg
     3721 gttattcttg tagaagagta tgctttttca cttactatac tattaggaga atttatgctt
     3781 agatatttat cataaagatc ttttgattct tgtataacat ttctatatat ttcttgcttc
     3841 catttaacga tttgttgttc ttgttttgct ttatcaactt tttccttgtc tctaatcata
     3901 ctagcttcga ttatgccttc catatcaaaa tttcctgaaa gacctgtaac tcttatagga
     3961 cttatacttg acataccttt atccctccat ctaattatat ttttagttga ttgtaaactt
     4021 catgccatgt atctctaatt tgttttacta ttggcaatat atcatcaatt attttaatat
     4081 ctttattttc atcagctatt gctaattcag attttataaa ttcataaact ttatacatat
     4141 cctttgccca ttgtcctgca ttttggtcta atgtagtcat tagttctgta aatatatttt
     4201 gaactcttac aagttcatta tgtgctttca aatagtcgtt atttaaaaga gcttctttgg
     4261 atatattggt atattttact gtgccatcta caagcatcaa tagtaattcc tcttttgata
     4321 acttatctaa ttcagctaaa tttaattcca ttatttgtac ctacttcttt cttctttact
     4381 ttttttatca gcttcatgcc atgtatcttt aatttgttct attacaggta acacattatc
     4441 tattacctgg atgtcttttc taatattggc tcttgaaagt tcatatctta caaaatcata
     4501 aactttgtac atatcctttg cccattgtcc tgcattttgg tctaatgtaa ccattagttc
     4561 tgtaaatata tcctgaactc ttacaagttc tctatgcgct ttctgtgtat ttttatctaa
     4621 gatagctgct ctggctattt tggtatattt tactgctcca tcaacaagca taagtaacaa
     4681 ttgttccttt gatgccataa aaacagcatt ttgtttataa gaattataag gattttctcc
     4741 atacatatat tacttcctcc aagcttatta ttttaatgac tttaaaattt ttatattttt
     4801 atttgtagct tttatattct cactctttac tttttcgtat atttcttttc ttaatatact
     4861 tatattgtta ggcgccgata tagctagttt cacattgttt ccatctaccc ctacaatttt
     4921 tacttctatg ctttctccta taagtactgc ttcatctttt tttcttgaaa ttactagcat
     4981 ccactatcac ctctcaataa tggatgcctt attgaatatt tatcatcctg taaaattatt
     5041 tgcattccaa aattagtttt tatatttatc actataggag ctttcatgtt cacagttgat
     5101 tcttcaaatg tctgaccaag agtaattata ttaaataata caacatcctt tggagatttc
     5161 acttctagtt tttctattgt ttcctgattt aaaactattt catattcatt gataatctca
     5221 aatggagata caacaacaaa tcctatacct tcttcttcag tagaattaaa ttcttttaaa
     5281 taatcattat cttctatatc taaaagctca tattctttta aattttcaaa acctaatata
     5341 ccttttttta atgtaacctt catcatatct gcgttatttt attatatgta atctaatata
     5401 gatacattaa ataactttcc tcctgtttgt agtgaagctt gataagttaa ctctgctgcc
     5461 tttaattgaa caagtttttc accataatct atttcttcat tttgtgacaa aatatccttt
     5521 aactgtatca aactatcatc gtttttttcc ttcatagcct ctactgtatt tgcttttgtc
     5581 ccatatatag acctaacatt tactgtattt tctattagtt gttccatatc gcccaaacaa
     5641 gttgttgtaa gctcttttat agcatcagca tctggatttg ctaatttttt tgatatgtta
     5701 tttaactctt caaatgggtc agtttttgtt gggtcaaatc caaggctact caatttaacg
     5761 ttataatcta cacttattcc ttgagctata tccatactca aagagtcatt taatttatta
     5821 agatttagac tagaattaaa ctttaaactc actgaaccat cagcattatc tatacattca
     5881 acaggtggta cccccgtatt tgaacctgaa aatatgtact tcccttcatg tgttgcattt
     5941 aaagcatttg caatttcttt cattttttca ttagtttcat tactaagagt ctttaattca
     6001 tcatctgagt atgcaccatc accacttgcc accaaagttt tctttatgtc tgataataat
     6061 tttccaagct gatctaatgc agtatcagta gtttctaacc aattccctgt atctttacaa
     6121 ttctcaacat aagtctccat agtctttatt tcactttttg agtttaatat ctttattgct
     6181 ttatatggat tatctgatag cttgtttatt tcttttgttg tattaacttg tctattaacc
     6241 ttatccaatc tttgtagatt atcttgtagt gagtttagat agctgctaga catcatacct
     6301 gtactgactc tcattataaa ttcccccttc tattaaatta ttccatttat tacaacatcc
     6361 agtagttgca cagctgttga cataactttt gcactagctt gatatgaacg ttgtattttt
     6421 ataagctctg tcatttcttc atccaaagat acacctgaaa cactcattct tgagttatca
     6481 atattattta taattttacg ttgatttgct tcatcttgat ttactttttg agaatctaaa
     6541 ccgattgatt ctgcaaaggc tttatagtgg tcacttattg tacttacttt tccaccagcc
     6601 accccaatat ctattttttc atcctttaac ttagcaattt ttgaagcttc atcagcagta
     6661 agctttaatt catttgggtt actctttata gctggattta tctttataat atcttcacca
     6721 tctacatttg aagttataaa aaaatctgtt actgttattc ctgcagacgt attataaaca
     6781 tcatttatct gtttagctaa tccatcagat acatctttta aatcgctttt atatgtatca
     6841 atcttaccat gcatttcaaa caagccttgt aaggtccctg atatacctgt tttccaagtt
     6901 ccatctggat tttttaattc attaagttct aatttaccat ctgcaagcat atttttaaca
     6961 tcactatttt caaagtctag tttaaaactc aagttatcta aaattctatc tctttcatca
     7021 agtaaatcat ttggtgtaga gccagaacca gatataatat caatagattt atttaaatct
     7081 tcaagacttt ttaacatgtc atttatttgt tttatctcag tgtctatatt agaatctata
     7141 tcttctttta acttatcaag cttcttataa ctttcactta ttaaagttgc taaattagat
     7201 gcgttttcta ccactagact ttttttactg tcactattta tatcctttga taatgactcc
     7261 catcctttaa aaaaatcatc tatacctgca gatattccat agtctgatgg ttcatttagt
     7321 atagtttcta tagtattgta ataatcatat tttgaaactg tcttaccata cttatgagct
     7381 tctgccctaa attggtaatc ataaaaacta tttctagctc ttgttacatc cgttacatgt
     7441 actccagtcc ctaattgtcc tcttgcaatt gaattactca aactaggcat atacattgga
     7501 ctatttgtgg caaactcggt cctttgccta gaataaccgt ctgtatctag attacttata
     7561 ttatgagaaa ttgtctgtat tgctttttgg tgcgcattca ttcctgaatt agctgtattt
     7621 aaaatggaaa ataatcctgc catatttctc ctcctaatat ttattttcta atatttccat
     7681 aagaattata agttgtagtt tgcttatcag ctggttttat gaaattaatc atttttttcg
     7741 taaaaataag ttctttcttt attagagagt catttgattc ttgttgattt ttagctaaag
     7801 caagaatgtg tttcaagtct gtatatattt cttttaagta tccatcatta ctattttcaa
     7861 caacttcact cattgaaata tcatcaccaa aaagttgtct tctctttatt tctatggatg
     7921 caagctccct gctaatatct tctagtttag gatttaacat atttaaattt tctaagtctt
     7981 tttcaactat ataatcaaat tgttctttta ataattccat catcttacta attatatttt
     8041 tttcttcata tattataact tttaagtctg taatcatgac tttatcctcg catatcctct
     8101 atcatttttt tcgcaatttc ttttggtttt attttgtaag ttccattttc taatgcattt
     8161 tttatttcat cgactttttt atttctaata ttctcatcac tcatatttga cacacttgcc
     8221 atttcctttg caaatttaga taactctatc ttgtctgttt tattaatttt tttaacctct
     8281 tccacttttt ttataccaga tgtattcttt aaataattat tcactacaat atttgaactt
     8341 acatttttaa tatccatagt ttcacctcca aaattacata cttataattt agttatcgct
     8401 cataaaataa aaatatttat ataattcaca catattttaa attttttata aaaatatccc
     8461 cttagctaaa ctcatacaga atagcctaaa gggatactat ttaaaatgct aatttacatc
     8521 tggatcttct attttagata atcttacaga taacctctca tttgtaataa tgctttctcc
     8581 ataagcaaac aatctatcgt ttgcatatat atctaatttt tcatccacat tcttacttaa
     8641 ttttattata gagccttctt ttaaatctaa tatatttttt attgtttctg ttgtatttcc
     8701 tatagaaaca gctaccttta cttttgcatt aaaaacattt tgattttctt ctataacacc
     8761 agatttttct tctattaatt gcccaaaatt caattcatat atatcctctt ttttgctatt
     8821 cataaaattt ttctccttgt ataaccttat ctatacatat ctatcaataa gccttgtatc
     8881 atgtcaacag tagaaacaat atctatgttt tctttttctt cactaattaa cttactagtc
     8941 atatcctcaa gtttactatc aacaatcttt acattcttat aatactgatg ctcccaaaag
     9001 ctactatttt tatcaagtga atacatatta tctacaacag attttaggta ttcttttact
     9061 gcatttttat atatactaat atcgccatag ttttgagtaa gtacaagcct ttctcctgcc
     9121 ttttttacac tttccaaatt tcgttttata tcttccaatg aagaagattg ttttaaagag
     9181 tttaatttgt cagaaaattt attactctct tttagttttt taacctccat atttatactt
     9241 ggacttatgt ttagcttatc tatatttatt cccataataa agcctaccct ctataatatt
     9301 tcataacctt aggaatataa ttttgtgttt cttttggcat tttatacagg tcagagattg
     9361 attctactcc tctacttttc attcttccag gaccaccatt atatgccatc aatcccattt
     9421 ctatgctccc tccaaacata tcaatgtatt ccttaatatg tctagttcca gcatctatat
     9481 tctgttctat attaaaaggg tctgtaaccc ctaagtctct gcagttttct ggcataagtt
     9541 gcataagacc cttagcacca gcagaagaaa cggtatttgg attaaaatct gattcaactt
     9601 taattattgc ttttataaga tttgaatcta cgttatattt ttttgatgct tgatttacag
     9661 cattttcaat tctcaacttt acatttttat cttttgtatc aacatctata aatctactag
     9721 tggtattatt tactacatta tcaagttcat taaataaatt ctcagaatta gacaataaat
     9781 tgtagctatt agaagtatgt tgattatttt gtgataatgc ttttaacaag gtcatcataa
     9841 tcatatcaaa tccagatgaa ttattatatg tacaattaca ttgatttgta gtattactac
     9901 cagataattg agtcaatgct aatatattaa ttagtgaact tatctcattc aatataaaac
     9961 gcccctttct aaattttaaa gttatactga taaaacagct ttccaccaag attcattttc
    10021 tatataccac ttaacagttt cttttatacc caaatcaaag tcatattttg ccttccaacc
    10081 taactcattc tctattttag ttgcatcaat agcatatctt aaatcatgac caggtctatc
    10141 atttacatag gatattaact cttcagattt acctaaaaga tttaatatca tttttactac
    10201 ttctagatta cttctttcat tatgacctcc tatattgtaa atctccccta tatttccttt
    10261 atgtattatt aagtctatag cagtacaatg gtcatataca tgtaaccaat ctcttacatt
    10321 ttccccattc ccatatactg gcaattgttt gttttccaaa gcatttaaaa tcataagagg
    10381 aatcaatttt tcaggaaaat gatatggccc ataattgttg gaacacctag atatagttgt
    10441 taacaatcca tatgttctat aataagaact taccaataaa tctgctgatg ctttagaagc
    10501 tgaatatgga gaacttggat tgatagaaga ttgttctgta aagaataaat cctttctatc
    10561 aattggtaaa tcaccataca cttcgtctgt agatatttga tgataccttt taactccata
    10621 ttttatagat gcatctaaaa gtacttgtgt tcctataatg tttgttttaa taaataaatc
    10681 aggattctca actgacctat ctacatgaga ttcagcagca aagttaacaa ctatatcaaa
    10741 cctttcttct tcaaatagct tatacacctg ctccctattt gaaatatctc ctttaacaaa
    10801 tttataattg gaattgtctt gtatttctat taatgtctct aaattaccag cataagtcaa
    10861 caagtctagg ttaacaataa aataatctac atacttgtta agcatataat gtataaaatt
    10921 actcccaata aatcctgctc ctccagttat cagtattttt ttcatatttt ttctcctaaa
    10981 tgttaattct aataaatttt tattaacgtc ttgtacaaat atctttaaaa cttttttgtt
    11041 ttttgtcctt ttctgataaa aatatttctt caattccttt taaaggccac tcaatagata
    11101 tttgcttgtc attccatata aagccattct caaattctgg gtaataataa tttgtacatt
    11161 tgtaactaaa tactgctttt tctgataaaa ctaaaaatcc atgtgcaaac ccttctggaa
    11221 tataaaattg ttttttattc ttatcagaca atattacacc ttcccatttc ccaaatgttt
    11281 gtgaatcttt ccttaaatca actgcaacat cgaaaacttc tccctctgta actctgacta
    11341 attttccttg aggatattta acctgaaaat gcattcctct taaaacgcct tttcttgagc
    11401 tagactcatt atcttgaata aaacacatat caagaccctg ctctataaaa tcctttttat
    11461 tatagctttc cataaaatat cctctttcat cataaaatac atttggttcg actatatata
    11521 atccttctat tttagtcttt ataacattga tttttcccat tataataaac tcccttaata
    11581 aaatttataa taaattttat ctaccatatt acatatcttc actagccaat tttaatagat
    11641 attgaccata ttcagttttt atcagtggct tggctagttt cattaattgt tctctactta
    11701 tatatccttt tgtatatgca atctcttcta aacatgctat atacagtccc tgtcttgttt
    11761 gaaccacttc aacataatta gaagcatcca atagaccttt atgtgtacca gtatcaagcc
    11821 aagccatacc tcttcctaga agttccactt ttagttttcc tcgttttaga tattcactgt
    11881 ttatagaggt tatttctaat tcacctctat ctgacggttc aactagcttt gctatctcta
    11941 caacatcatt atcataaaaa tataatcctg gtatagcata attagattta ggaactgtag
    12001 gtttttcttc tattgacaat acattaaaat ccttatcaaa ttctacaact ccaaacgaat
    12061 ttggattact tacatgatac ccaaatatag ttgctccttt tcttttaact gcattctcca
    12121 acctttcact aaatccatat ccataaaata tattatcccc taatacaaga gacactctgt
    12181 catttcctat aaattcttct cctattataa acgcttctgc aattccattt gctttctcct
    12241 gaattgcata tgttatgtta agccctagtg aacttccatc tccaaataat tcttcaaatg
    12301 cttttatatc tcttggtgta gatattatta atatatcttg tatccctgcc aacataagaa
    12361 ccgacatagg gtaatatatc attggtttat cgtatattgg aagtgtttgt tttgatatac
    12421 attttgttat aggatatagt cttgtaccag accctcctgc taaaataatt cctttcatct
    12481 ttttccatcc ttaaatttgt ttttgtcata ttatgattat cggataaata aaagaaatat
    12541 ttatattttt tataatatct tattttcttt tctcaaaata atacatttac ttttcattta
    12601 tatcatatct ttctaaatat tcttcaatgg attcttccca ctttctaaaa gaatttaaac
    12661 caattaactt caacataaaa ttatccaata cagaatatag tggtcttgga gccttacatt
    12721 taaattcgtt actcttaatt ggatttactt ttatatctat attttttagt ttaaatatct
    12781 ttttggcaaa atcataccaa ctacactcac cttcacatgt agcatgataa actccataat
    12841 gttctgtttc catgattttt attataattt ttgctaagtc tactgtactt gttggagttc
    12901 caaactggtc attaaccaca ttaacctctt tattttctaa agaaagtttt atcatggtct
    12961 ttacaaaatt attcccatta ccatataacc atgctgttct caatataaag tacttatgtg
    13021 agaattgttc aacaaatttt tctcccataa gtttactttt tccatataca gaattgggtt
    13081 cagttttatt gtcttcccta tatggatatt tagaaaaacc attgaataca tagtcagttg
    13141 atatgtgaat aagcttagca ttaacttttt ctgatgctat tgctagattt ctcactccta
    13201 aagcatttat tttgtatgca atttcgatat tatcttcaca tgcatcaacc tttgtatatg
    13261 ctgcacagtt tacaactaca tcaggtttat tatgcaaaat aaaattatct acattagtct
    13321 gattggatat gtccaaatca cttcttgttg tagctaatat aacatatttt ggttggttta
    13381 tactttgatt gattgcttct aattggttaa ccaattcttt tcctaattga ccattagacc
    13441 ctgttattaa tattttcatt aattatgttg tcctccaata aatattcaat tttacttaat
    13501 aattaattat aacacagctt gtattttaac ttaggttgaa cttattattt cttagataaa
    13561 taaaaaagag atgtaatatt aaatgattat catcatatta ttacatctct ttaaatctaa
    13621 atatctaaat tatatattct agctatcaat taaacatcaa agctttatct aaaatattta
    13681 actccagatt caaatatctt ttggtctttt tcacctataa tattcttata tacttcctca
    13741 ccaattcttt cagaatgtcc cattttacca aacactctac catcagcact agttatacct
    13801 tcaacagcat agaatgaacc atttggatta aactcaatat cataagtagc ttcattatta
    13861 aagtcaacat attgagttgc tacttgtcca ttagcaatta attctctcat aacatcttca
    13921 ctggcaacaa acttaccttc tccatgagaa attgctatat tatgaatatc tccaacatta
    13981 gtttgagcta accaaggaga tttattagat gaaattctag ttctagcaat cttagcttga
    14041 tggcgaccaa tattattgta agtaagtgtt ggagcacttt cacttggaac tcttatttct
    14101 ccatatggaa ctaaccctaa tttaattaat acttggaatc cattacagat acctaacatt
    14161 agtccatctt tttgagttaa aaattcattt atagcttctt gcactctagg atttctaaat
    14221 acagtagcta taaatttacc agaaccatct ggctcatcac cagcactaaa tccacctggt
    14281 aacattatta tttgagaaga ttttatttcg tttactattg tatctataga atcttctatg
    14341 tctttataag ttaaattctt aaatactctt atacttgcat tagctcctgc tctttcaaat
    14401 gctcttgcag agtcatattc acagtttgtt ccagggaatg ctggtataaa tactcttgga
    14461 gtagctatac ttaatgatga ttttttagct tctccttgtg atatataact tatagtttct
    14521 atctttgatt ttacttcttc tgttttagtt gggaatatag gctctaaagt actacaatgt
    14581 gctttatata attcatctaa tgcaagttct tcacctttga taaatatact agtttcttta
    14641 actgtacttc caagcacaac ataattgtat ccttctaaca tacttaaatc atcatttgct
    14701 aattcaagta ctatatttcc ataactagcc tcaaataatt tatcatctgt aaatgctttt
    14761 tcagcctctt cactaaactt aaatcctatt ttattaccaa aagccatttt acttattgct
    14821 tcacctacac ctgcaaatcc taatgcatat gttgataaaa ctttatttcc atgtataagt
    14881 tctcttactt tatctaagtt tctctttaat tcctcaaaat ctacaacatc attttctgcc
    14941 ttgtttacac ataacattac tacttttgag tctgcttttt taaattctgg agatactact
    15001 ttcttcgcat ctactgtgtc aacagcaaat gaaactaatg ttggtggaac ttcaatatct
    15061 ttaaatgtac cagacatact gtcttttcca cctatagcag gtatctcaaa tttactttgt
    15121 gcataaaatg ctcctaaaag tgctgagaat ggtttacccc atttttctgg attgttacca
    15181 agtttttcaa aatactcttg gaatgtaagt cttgttgtac tataatttcc tccaatagca
    15241 accaatttac acacagattc tactactgca tacaaggcac catggaatgg gctccatttt
    15301 cctacctttg gattgtatcc ataagtcatt attgtagaag tattagtttc tcctccaagt
    15361 actggtatct tagcaaccat accttgtgtt ggagtcgctt gatactttcc tccaaatggc
    15421 ataagtactg tattaccacc aattgtatta tcaaacatct ctactaatcc tttttgagaa
    15481 caaacattaa ggtcagatag tacttttgta aacttgtctt tcatacttgc acacttaaca
    15541 tcttttacta tttcattaga gctaaagaat gtattttctt catctacttt agtaacatga
    15601 acatcagtag tttgttttac tccgtttgta tctaaaaact ctctatttat atctacaatt
    15661 gcttttccat tccagaacat tcttaaatat cctgtatctg ttactgtagc tacatgcgta
    15721 gcttctaagt tttcttctac tgctaattgt ataaatttat ctttattttc tttttttatg
    15781 gctactgcca tacgctcttg tgactctgaa attgcaagct cagttccatc aagtccatca
    15841 tattttttag gaactaagtc taagtttata tctaagctat cagcaatctc tcctatagca
    15901 acacaaactc cacctgctcc aaaatcatta catcttttta tcatttgagc aacttcttta
    15961 tttctaaaga atctttgtat ttttctttcg ttaggagcat ctcctttttg aacttctgca
    16021 ctacaagtta agattgactc ttcactatgt tctttagatg aaccagttgc tcctccacag
    16081 ccatctcttc ctgtttttcc tccaagaaga acgattacat ctcctgcttc aggtctttct
    16141 cttactacat tctcttttgg tgctgctgct ataactgcac ctatttccat tctctttgca
    16201 acaaagtttt catcatatac ttctgcaact tgtccagttg taagacctat ttggttacca
    16261 tatgaactat atccatgagc agcttctgta gttatttttc tttgcataag ttttccaggt
    16321 aaagtatctt ctaaagtcgt tcttgggtca gcacttcctg taacacgcat tgcttgataa
    16381 acataacttc ttcctgataa tgggtctctt atagctcctc ctaaacaagt agctgctcca
    16441 ccaaatggtt ctatttcagt tggatggttg tgagtttcat ttttaaacat tactagatat
    16501 ttttccatct taccatcaac ttcaacatct acgtttatac tacatgcatt tatttcttca
    16561 ctttcatcta ggtcatttaa tttcccattt ttctttaatt cttttactgc aacagtggct
    16621 atatccatta aacatatatc tttatgtctg ttcacatata cattgtctct tgagttaaga
    16681 tacatttgat acgcttcttt tactatatca ttaaacttac catcttctat ctttacattt
    16741 tctatttcag tcataaaagt agtatgtcta caatgatcag accaataagt atctaatact
    16801 tttatttctg ttatagttgg atttctcttt tctgtatctt taaagtattt ttgaacgtgt
    16861 tgtaagtctc ctaaagtcat tgctaatcct ttttcactta caaatgtttt taatccattt
    16921 tcatctaaat ctataaatcc atctaatact tcaactgttg ttggtatttc agtttccatt
    16981 tttaacgttt ctggtttttc taaagatgct tctctactat ctactggatt tatacagtaa
    17041 tcttttattt tagaaaactc ttcatcagtt attttgcctg ataatatata aacttttgct
    17101 gtattaatag caggtcttat cccttgattt acaatttgta cacattgagc tgcccaatca
    17161 cctctttggt cgtattgacc aggaagaaat tctattgcaa atactctttc atcatttaat
    17221 tttggtattt cttcataata tactacatct aaatttggtt cagagaatat tgtctttgct
    17281 gcattttcat aaacttcttc tgatattcct tctacatcat atctatttaa tattcttaaa
    17341 ttttctatat tatctatgtg taaactttct actaagtctt tttttaaagc tttagcctca
    17401 aggtcaaaac cttctctttt ttcaactaat actcttctaa ccattgagtc tttattttca
    17461 gtattcaaca taccttatcc tccattttag aaaaattatc ttcctatgtc tgttctatag
    17521 tgcattcctt caaagtttat cttctttatg ttttcataaa ctttttctgc tgcatcctta
    17581 actgtagttg actttgcagt tataccaatt actcttcctc cattggtaac caaattacca
    17641 tcaaacatct tagtgccact atggaatact actatatcat catccaagtt ttcaagacca
    17701 gttataattt tccctttttc ataactgtct ggataccctc ctgatgtaag cataacacat
    17761 acagcttcat catcactata gtttatttct atatctttta acttattatc tagtatagct
    17821 tccattatct catgtaaatc tgtttctaat ctaaataaaa cagattgtgt ctcagggtct
    17881 ccaaatctta cattatattc aagtactttt tctccatctt cagttatcat aagaccaaca
    17941 aataaaatac ctttaaaatt aagtccatct tttttgaagc cttctaaagt tttttctaat
    18001 actgtctctt ttactttatt tgctaattct tctgtatata tttcactagg tgaaaaagtt
    18061 cccattcccc cagtgtttgg accttgctca tagttgttta cttttttatg gtcttttgca
    18121 cttgccatag gaactattgt atcattatct acaaatgcta gaattgaagt ttctattcct
    18181 tttaaaaact cttcaattac tattttatct ccagcagctc caaacttctt atctgacatc
    18241 atttctttta aagttgctat agcatcttct ctattttcag gtattacaac accttttcca
    18301 gcagctaatc cgtccgcttt tattactact ggatatccaa aactatctat ttctgatata
    18361 gcttcttcta agtttgtata ttctttatat tttgcagtag gtatgttatg ttttatcata
    18421 aattcttttg aaaaagcttt acttccttca agttgagcac attttttatt aggtccaaat
    18481 actcttaatc cttctttttc aaactcatct actattccca taactagtgg tacttctggt
    18541 cctactattg tcagacctat ttcattttct ttggcaaatt ttaaaagttc ttctatatta
    18601 gtatcaccta tatttacaca ttctgctacg tttgcaattc ctgcattacc tggtgcacaa
    18661 taaatttttt ctacattttt ttctttgctt agtttccagc atatagcatg ctctcttccg
    18721 ccaccgccta caaccaatat cttcataata tcctccttag tgtttgaagt gtcttattcc
    18781 tgtaaatacc ataaccatat catacttgtc acaagcttct attgaatctt tatcttttat
    18841 agagccacct ggttgaacta ctgctgatat tccatattca tgagctaatg ttacacaatc
    18901 atcaaatggg aaaaatgcgt ctgacgctaa aacagctcct gtaaaatctt tatctttgtt
    18961 attttcaaga gcatttttta atgcccaaat tcttgatgtt tgtccacaac ctaaagctag
    19021 agtttgtcca ttttttacta ttgcaattgc atttgatttc atatttttta ctactctcat
    19081 accaaattcc atgtccttaa gttgtgcatc tgttggtttt tcctttgtta cattttcact
    19141 tttttctgct aatatattgt ttctatcttg tatcaataat tttccatcta aatatttcat
    19201 ttcatatggt tgtaaactat tttctatttt agctaatttt aatattctta gatttttctt
    19261 ttgtttaagt atctctaaag cttctggttc aaaatcatat gctactacta tttctaagaa
    19321 tatttcattt aatttctctg ctgtagcttt atctatagta gaagttattc ctactattcc
    19381 tccaaatatt gaaaccttat ccgcttcata acattttgta tatgcttcaa aagcatcttt
    19441 tcctaaacct accccacatg aattagcatg ttttatagct actgaaacta cttcttcact
    19501 atctttaaat tctctcatta cttctaaaca tccatgtaaa tcatttaagt tgttaaaaga
    19561 aagttcttta ccgcctaatt gctcataatt taagattgga ttttttgcat ttgactgtga
    19621 atataagaaa ccattttgat gtggattttc tccatatctt aaagtttgtt ctttttggaa
    19681 agttaaattt aatatgtcag gatacgtatc tccaacttct ccagcaaaat aactagatat
    19741 taacgcatca tatcttcctg tagttgagaa agctttatat gataattttt ttctgtcttc
    19801 taatgtcatt gtatctgttt ttaatttttc tattatagaa tcataatctt ttatatctac
    19861 tactactatt acatccttat agtttttggc agcagaacgt atcatagatg gtccaccaat
    19921 atctatattt tcaattattt catcatgact ttttccagct tttaaagttc cttcaaaatc
    19981 atatagattt acagctacta aatctattga atgtatttta tgttcattta cagtttctac
    20041 atgagattct ttatctcttt tataaagtat accaccatgt atatatggat ttaatgtttt
    20101 tactcttcca tctaatattt ctggaaaatt tgtaacatct tcaacttgca ttacattaac
    20161 accattttct tttaatgttt taaatgtatt tcctgtagat ataatttcat aatctaattt
    20221 atttaactct ttagcaaatt ccactacacc agtcttgtca gttacactta ttaaagctct
    20281 cttactcatt tataaacact ctcctacctt gaagttttaa tttattttca caaaaaagac
    20341 ttatactttc ttttaaaatc ctatgttcaa cttctaacac tctttttgca agtgtctttg
    20401 catcatcatc ttgatttacc ttaactacat cttgcattat gattggtcca gtatcagcac
    20461 cttcatcaac aaaatgaact gtagcaccag ttactttagc tccgtaatct ataacacctt
    20521 gatgaacttt ttctccatag aatccagctc cacaaaatga aggaattaat gatggatgta
    20581 tgtttatcat tttgtttcta aactcattaa ccagctttgg gctgattatt tttaaataac
    20641 cagccaaaac tactaaatct attttatttt ccttaagaat ttctattatc ttgtcttcat
    20701 cagtttcaca aattgctttt atgttatgat ttttagctct ctctagccca taagcacctt
    20761 gcttacttga gataactact ttaacctgac cttttatttc tccagactca gtaccatcta
    20821 taactgcttg taaatttgtt cccccaccag atattaaaac tcctatattt agcataattc
    20881 tacaccttca tgactatcta ctacttcacc aattatataa gctttatctt ctaataattc
    20941 actatatttt ttatctacat aatcagcatt gtcattttct ctattattta taaagtttac
    21001 aacatcatca gcattttctt tatctactat taaaactagc ccaataccca tgttaaaact
    21061 cttatgaagt tctctctcat caacagcatt aaaaccttct atcattttaa atataggagg
    21121 cttctcccaa gattttttat tgatatctag tcctaatcct ttaggtataa ctcttgttat
    21181 attttctata agccctccac cagttatgtg agctacagct tttatgtcat gtttagctaa
    21241 tacatctaaa gctaatttaa catatatttt agttggtgtt aaaagtgctt ctccaactgt
    21301 cattcctagt tcttcaacat atcgttccat tttataattg taagtttcta aaaatatctt
    21361 tcttataaaa gaaaatccat tgctgtgtac tccacttgaa gatataccaa ccaatacatc
    21421 tcctgattta acattgtttc cagaaacaat cttttctttg tctgctattc ccacagaaaa
    21481 tcctgctaaa tcatagtcat cttctccata cattccaggc atttcagcag tttctccacc
    21541 aattagtgca caaccagaca ttttgcaacc atctgcaatt ccaccaacta ttttttctat
    21601 atgctcaggc actaatttcc caagagctat atagtcaagg aagaataatg gttttgctcc
    21661 ttgacaaatt aaatcattca cgcacattgc aactaaatct attccaacag tatcatgctt
    21721 gtccatcatc tgagctattt ttaacttagt cccaacacca tctgtagatg caagcaaaac
    21781 tggttctttc atacctacaa aatcttttaa actgtataat ccactgaagt tacctaaatc
    21841 tccaactaca tttccatcat aagttccttt tattttacct tttataaggt ctactgctct
    21901 atttccttca tctatatcta cacctgattc tttatatgtt aacatgaaag tagttcctcc
    21961 ttatcaattt tcttaactgg gtattctcca tcaaaacaag ctttacagaa agtagactta
    22021 tgttctgttg catctaacat tccttctata tctaggaatt ttaaactatc acatccaata
    22081 tattctctca tttcttctac gttgttactt gatgcaatta atttagagcg atttggagta
    22141 tctattccat agtaacaaga gtaagctact ggaggagaag ttattctaag atgaatttct
    22201 tttgcacctg cttctcttaa agattttact aattgtttag atgtagttcc tcttacaatt
    22261 gagtcgtcta ctaaaattat agattttcct tttattattg ttgaaagagg atttaatttt
    22321 atttttactg ctatttctct ttcttcttgt gtaggtttta taaatgttct tcctacatat
    22381 ctatttttaa ctaagccttc acttattttt aatccactag catttgcata accaatagct
    22441 cctggccaac ctgaatctgg aactggaact accatatctg cctttacagt ttcattctga
    22501 gctaatctct caccacattt aattctaaat tcatatgcat ttacattgtc tatagttgca
    22561 tcatttcttg caaagtatat gtgttcaaat atacaacttt tcttaactgg tttataattt
    22621 tctgaataaa aatatgattt tagttctcca tctttgacta ctattatctc tcctggctct
    22681 acatctctta taacttctcc gccaagtata tctattgcac aattttctga tgcaaatata
    22741 tattcatcac cctttttacc aagtacaaga ggtctgaatc catgtgggtc tctaactgca
    22801 accaattctt cttgactcat tattacaaga gcatatgcac cttttatata atccattgtc
    22861 acttttaaac tttctactat atcccctttg taatatctag caagtatata taaaatcact
    22921 tctgtatctg aattagtttg gaacatatat ccatcttctt ctagcatatc ttttaaataa
    22981 tttgcattaa ctagatttcc attatgagct atggctaatt gtctttttct acaacttcca
    23041 actaaaggtt gacaattaga aacatgactt cctccagctg ttgaatatct aacatgtcca
    23101 atccctatat ttccttttaa cttagataat tcattttcct taaaaacatc tccaactaat
    23161 cccatatctt ttttgtactt aatttctcca tcatctaaaa gagctaaacc acagctttct
    23221 tgccccctgt gttgcataga atataaagaa taatatagtt ctttagttac atctttatta
    23281 gagtaaatcc ctaaaactcc acacatacat ttgcctccta tttattattc attcttgaaa
    23341 gtacttccgt ataaccttct acaaggtctc caaggtcttt tctaaatctg tccttatcta
    23401 acttttcatt tgtatttaca tcccaaagtc tacaagtgtc tggtgatact tcatctgcca
    23461 atattatatt tccttcagaa tccttaccaa attctatttt aaagtcaact aactttaggt
    23521 ttaattttaa gaaaaatgct tttagtaact catttatttt taaagtttct tctcttaaga
    23581 attttaactc ttctctagtt gctaacttca ttgccactgc ataatcatca tttagcattg
    23641 ggtcaccata agcatcattt ttgtagctta tttcaaaaat tggctcatcg aaaactacac
    23701 cttcttcaag accaactctt ttacagatag aaccagcagc aatatttctt actattactt
    23761 ctaatggtaa aatctctact tttttaacta acatttctct atctgataaa ctttttataa
    23821 agtgagtatt tatattattt tcttttaaca tttcaaagat tattgttgat atcttattat
    23881 ttagtatacc ttttgaagat atttctgctt ttttttctcc attaaatgct gttgcatcat
    23941 ctttataata aacaacatat tcattttcat tatctgtaga atatacttgt tttgcttttc
    24001 cttcatataa taacatttct gctaccccct taaattattg tcgtcttcta acaccttttg
    24061 tgccatctct tctctatatg atttcaaatc ctcttttaat tttgggtatt ttaaagtcat
    24121 tatttgtaat gccaataaag ctgcgtttaa acctaagtct attgtaactg ttgcaactgg
    24181 gattcctttt ggcatttgaa ctattgataa aagtgagtct agtccatcca tagttgatga
    24241 tttaataggt aatcctatta caggaattaa tgtttgtgaa gctattacac ctggtaagtg
    24301 tgctgctttt cctgctgctg ctattattac atcagtatca tcttctattt cttttaggaa
    24361 tattgaaagt tgttctggtg ttctatgagc tgataatgct cttgctacaa cttctattcc
    24421 atatttttca agcagtttta tcccttcttc taattttgga taatctgatt ttgaacccat
    24481 aactactgct actttcatta gacttcccct ctccgtttat aagttatttt acactacaaa
    24541 tcattcgtat tattatacga attatacacc ttaaatttta tattaatata catatattat
    24601 caactctttt ttgaaatttt ttaaaaaaaa tatttcttgt aattttttca ttcgctacgt
    24661 atcgaacttt atttatgttt ttttgttaaa ataaaaaaca gattatctca aaattggttt
    24721 tctaattaaa ttttaagccc aatactcttt tttaaatctt gtatattatt ttttattact
    24781 tcttttttaa ttaatacttt ggatttatag tcttgttttt tagttctgaa tgttactttt
    24841 acttagataa aaccaacaca aagtttattc tttatataag taatagctaa aatttaaagt
    24901 aatgatatta tataaataga aataatttta tttaaaggag aatctgtatg ttagaagtta
    24961 taaaaaatag acgttcaata agaacatata tagataaaaa tatagaagaa gataaaataa
    25021 ctgaaatact aaaatcagct atgcaagcac cttcatcaaa aaatgctcaa ccttgggaat
    25081 ttattatagt agatgataag gaactgctga aacagctctc aaagtcacag catagagcaa
    25141 agcatataga atttgcacca ttatgtatag tagtgcttgg aaatagagat aaattcttaa
    25201 aacctggtaa atggatacaa gatttaggtg cttgcacaca aaatttatta ttagaagtta
    25261 ctaatcaagg tctagctgct tgttgggcag gagtatttcc taaaaataaa gtggtaaata
    25321 aagttagaca aactttagac ttaccattaa aattagttcc ttatgcactt atatctattg
    25381 gatatagtga agaaaaaaat gagtttatag accgatttga tgaaaataaa atacatagaa
    25441 atgtatataa aaatagataa tgtttagtaa tataaatgaa aatacatagt aaaaatgcct
    25501 ttagaataca tttgtattct aaaggcatta gctttgacta aacacctaat tagcctctaa
    25561 ggtaaaattt aactatcatt tattatttat ataccaattg aatattatat ccttcaatat
    25621 ttttatttat ttcaaatata ttaagaatat cctacaatta aagttagcta tttttcatta
    25681 aagtatttta ttgacacact ccccatagct aaagcaaggg gattcttggt ggctaacaca
    25741 cttatttata cttaaacagt acaacagagg tggttacaaa gccaagctac ttcggacggt
    25801 gtcttcgcca ctactacc
//
LOCUS       DAGSHT010000034        25435 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGSHT010000034 DAGSHT010000000
VERSION     DAGSHT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.035"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            348..1721
                     /locus_tag="MJB72_001301"
     CDS             348..1721
                     /locus_tag="MJB72_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8262425.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1977..3044
                     /locus_tag="MJB72_001302"
     CDS             1977..3044
                     /locus_tag="MJB72_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBY8262426.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3188..3496
                     /locus_tag="MJB72_001303"
     CDS             3188..3496
                     /locus_tag="MJB72_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBY8262427.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3489..4919
                     /locus_tag="MJB72_001304"
     CDS             3489..4919
                     /locus_tag="MJB72_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBY8262428.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4997..5371
                     /locus_tag="MJB72_001305"
     CDS             4997..5371
                     /locus_tag="MJB72_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBY8262429.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5365..7569
                     /locus_tag="MJB72_001306"
     CDS             5365..7569
                     /locus_tag="MJB72_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBY8262430.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7569..8978
                     /locus_tag="MJB72_001307"
     CDS             7569..8978
                     /locus_tag="MJB72_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBY8262431.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8963..10027
                     /locus_tag="MJB72_001308"
     CDS             8963..10027
                     /locus_tag="MJB72_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBY8262432.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            10057..11478
                     /gene="nhaC"
                     /locus_tag="MJB72_001309"
     CDS             10057..11478
                     /gene="nhaC"
                     /locus_tag="MJB72_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBY8262433.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11907..12371
                     /locus_tag="MJB72_001310"
     CDS             11907..12371
                     /locus_tag="MJB72_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY8262434.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12605..12967
                     /locus_tag="MJB72_001311"
     CDS             12605..12967
                     /locus_tag="MJB72_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY8262435.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13562..14398
                     /locus_tag="MJB72_001312"
     CDS             13562..14398
                     /locus_tag="MJB72_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262436.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLAFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            14482..16596
                     /locus_tag="MJB72_001313"
     CDS             14482..16596
                     /locus_tag="MJB72_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBY8262437.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            17137..18090
                     /gene="blaCDD"
                     /locus_tag="MJB72_001314"
     CDS             17137..18090
                     /gene="blaCDD"
                     /locus_tag="MJB72_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBY8262438.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            18434..19117
                     /locus_tag="MJB72_001315"
     CDS             18434..19117
                     /locus_tag="MJB72_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262439.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            19114..21774
                     /locus_tag="MJB72_001316"
     CDS             19114..21774
                     /locus_tag="MJB72_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262440.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(21955..23136)
                     /locus_tag="MJB72_001317"
     CDS             complement(21955..23136)
                     /locus_tag="MJB72_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8262441.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(23136..23798)
                     /locus_tag="MJB72_001318"
     CDS             complement(23136..23798)
                     /locus_tag="MJB72_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8262442.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIICGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(23944..24582)
                     /locus_tag="MJB72_001319"
     CDS             complement(23944..24582)
                     /locus_tag="MJB72_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8262443.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            24761..25366
                     /locus_tag="MJB72_001320"
     CDS             24761..25366
                     /locus_tag="MJB72_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8262444.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         9877 a         2933 c         4469 g         8156 t
ORIGIN      
        1 ctataattat attatggact aaaaaatcca atgagctaat ttattgtttt tttatatttg
       61 agagattaca acttattagt ttgtatatct ctttttttaa taaaatcaat aatatataaa
      121 actattggca tatttaatga caattcaaat attgtatata accataatga aagatatata
      181 aataaaattt taaatatact taaaaatata ttaatataat gtacataaaa aatatcataa
      241 atatatgata agataaatac aatagcaaca ttttattaat tttttgggtt tttatatatt
      301 taggtactgg ggtgatatag ttgaaccaag agatattttt taaaataatg aatgaaatat
      361 tggaaaatat tgatgaaggg gtacattttg ttaataaaga cgggaaaact ataatctaca
      421 ataattctgt acaaaaaatg gaaaaaatga acagacacga tattttaacc aattcatttt
      481 ttgatatagt caacaaaatg aaaataaaca ggagtacact attagaagtt ttggaaaaaa
      541 gggaagttat caaagacaat attcaaaaat atctggataa aaatggaaaa gagataacag
      601 caattaatac tacaatacca attgatatta aaggcgagtt tgtaggggca cttgagatat
      661 caaagaatat gacaacgata gaaaatttac taaatagaaa ttataaaaaa aatttagtca
      721 ataaagttag tagtagacaa aaaaagggtt atgaatttga tgatatttta ggggaaagta
      781 atttgattaa agaagcaata gctaaatcta agaaagcttg caagtctgat gcttcagtat
      841 ttatatacgg tgaaactgga acgggtaaag agttatttag ccagtccatt cactatggaa
      901 gtaataggaa aaactatcca tttatagcac aaaattgtgc agctttgccg gaatcactat
      961 tcgaaggtat tctttttgga actagcaagg gaggatttac aggagcaata gataggccag
     1021 gcttatttga acaagctaac aaagggacat tgttattgga tgagataaat tccatgccta
     1081 ttatgttaca ggctaaacta ttaagggttt tacaagaagg ttatgtaaga agggttggag
     1141 gaactaagga tatacctata gatgtaagaa ttattgcaac gacaaatgaa agtccaaagg
     1201 taattttaaa cgagcataaa atgcgaaaag atttatatta taggcttaac ataattcata
     1261 taaaaattcc tcctcttaga gatagaaaag aggacatacc tctattgtgc aaaaaattta
     1321 tttcaaagta taataaaaag ttaaataaaa aagtggtagg aatttcagat gaagctatta
     1381 aaattttaaa aaattacgat tggcaaggta acataagaga gctagaaaac ataatttatt
     1441 gtactatgag tatgatggaa gatgaggtac aaatagaaac aagtatgatt aatctaaacg
     1501 actattacaa tcttaaagag aataaagaga gtaacaatta ttctttaaac gatttggagg
     1561 acaaaacact tagtaagatt atcagtgaga tagaagaaaa gtatatatat gaatcattag
     1621 aaaaggcatc ttacaatata actaaagctg catcaatttt aggtatgaat aggcaaaatt
     1681 tacagtataa aataaaaaaa tataatatca aaatattata aataagcaag ataaaaaaac
     1741 aaaatcaaag taaaataaaa aagcaaaata gaggagcaaa attattataa aagagcaaaa
     1801 tattttgctc ttttttttat ttctcataag aaatattatt ttttatttat gaaaatacat
     1861 aataaaataa ttatagtttc aacgttccaa taaatattta ctttaatttt aaagttggca
     1921 cgatttatgc tttaactaat tattgagaat atataaattt ttaggagggt gtatatatga
     1981 gaaaagtaag agtaggaata tggggatttg gagctatggg aataggtatg gctaatatga
     2041 ttctaaagaa agaaggtata gaaatagtat ctgtttgtag tagaagtact tcaggaaaaa
     2101 gtatgtataa tgttttagga attgaaagag gagaaagacc agaagttatt atcaataaaa
     2161 attatgaaga agtttttaga gaaaaaagtg tagatgttgt tttgttagca actgattcat
     2221 ttactaaaaa ggcttttgat aaaataattt tcttattaaa tagaaaaata aatgttatat
     2281 caacagcaga acaaatggca tatccacaag ctgatgatgc agatttagct aaaaaaatgg
     2341 atgaagttgc aaaggaaaat ggagtaagta tacttggaac aggaataaat ccaggatttg
     2401 tattagattt actagtattg gcgctttcag gcacttgtga agaagttact agtataaaag
     2461 ctaagagagt aaatgattta tctccatttg gaaagtctgt tatggaggaa caaggtgttg
     2521 gagtaacaag ggaagaattt ataaaaggtg tagaagataa gactatagca ggtcatgtag
     2581 gttttgtaga atcaataaat atgatagctg atggattagg ttggaaatta gataagatag
     2641 aacaaacaaa agagccaata atgactacag tagatagaaa atctaagtac ggagaggcat
     2701 tggctggaaa tgttgctggt tgtagacaat gtggatatgg atatgtaaat ggagaagttt
     2761 taattgaaat ggagcatcca caacagataa taccagaagc agaaggtatt aaaacaggag
     2821 actatgtatc aataaaaggt ataccaaaca ttgatttaca aataaatcct gaaatacctg
     2881 gaggagtagg tacttatgca atgatagtaa actctattcc attaataata aatgctagac
     2941 caggtcttaa aactatgttg gatataccag tacctagagc aataatggga gatataagaa
     3001 atcagataga agttgaattg gaagaagaat caaaggctaa ttagtaagat aacagctaac
     3061 taattagtaa gatagcaact agctaattag taagataaca gttagctaat tggtaaaata
     3121 atgtttaatt taaaataaca gtaaattaat tagtaaggca atagtcaatt aataagggga
     3181 tgaaaaaatg gatgctaaaa ttaatgattg ggttattata cacaatatag tattgactcc
     3241 agaagaaaga gctccacaag ttccagagga tactaagaaa gtatccttag aaatgtgggt
     3301 taaaggtttt atacaaagtg atgcttctat aggcgactta gtagaagtta aaacaattac
     3361 aggcagactt gtaaaaggta atttacttaa aataaatcca tattatactc atgattatgg
     3421 taaatgtata ccagagttac tccaaatagg aatccaagct aaagaaatat tatttggagg
     3481 tgtatataat gaataattca acatcaatga aagatatgag ttatcaagct gttatgggaa
     3541 gaaataacga aataatgaaa aatgcaatcg gattggatta ttcttctttt gaacaagagg
     3601 gaatagggtt tgattatgag aaaatgatga gtgaaacagg atatacatta caagatattg
     3661 aagcaattca atcacagtat gctgttggaa atactccttt aatagagctt aaaaatttga
     3721 ctaaattagc tagaaaatgt gctaaagaag gtaaaggagc tagaatattt gtaaaagatg
     3781 aagcaatgaa tgcatctggt agttttaaag caagaagagc agctactgca gtatatcatg
     3841 ccaaacaaat gggatataaa ggtgtaatag cagctactag tggtaactat ggtgcagctg
     3901 tagcatcaca agcggcaatg cagggattaa aatgtataat agttcaagaa tgttatgatt
     3961 ctaatggtgt aggtcaacct gaaattatag aaaaagcacg aaaatgtgag gcatatggag
     4021 cagaagttgt tcagttaact gttggaccag agttatttta tacgttctta gttttattag
     4081 aagaaacagg ctattttaac gcatcattat attcaccatt tggaattgca ggtgtagaaa
     4141 ctttaggtta tgagttagct atccaattta gagaaaagta taaaaaagac cctgatatag
     4201 ttgtttgtac aaatgcaggt ggtggtaatc ttactggaac tgcaagagga ttgataaaag
     4261 ctggttctat taatactaaa gtagtaggag caagtgtaga tttaaaagga ttacacatgg
     4321 ctagtgataa tcaatttaat aaaaaatcat ttacaacagg acatacaggt tttgggatac
     4381 catactgtac atggcctgat aggtcagatg tacctaggtc tgcagctaga ccacttagat
     4441 atatggatag atatgtatta gtaaaacaag gagaagtttt ttatacaact gaattattgg
     4501 cacagctaga aggtatagaa agaggtccag caggaaatac atctttagca gcagcattct
     4561 ctttagctca agagttagat gaggataaag ttatagtggt acaagaaaca gaatatacag
     4621 gcgcaggtaa acatatatgt ccacagctta cttttgcaag agaaaatgga atagatataa
     4681 aatttggaaa tccaagagaa gaaattgcag gagtaaactt aatattacca gaaagaccag
     4741 aattattgaa atgtgttgat attgatatga ataaaataag aaagtcattt ataaaaaatt
     4801 gtatatctaa taatcacatt gataatattg ataagctaag taatcgagat atagaatttt
     4861 taatggaaga agtaaaaagt tcaagagatt ttgttgttga tgtattaaat aatttatagt
     4921 aattattgtt ttttaattta atataaaata aaaattcaga aaattcgatt aaatcaatac
     4981 gaatggagga atgttcatga aaaaaagaga ggatgatttt gaagttagaa gaaaacatct
     5041 tcaagaactg agtgatgatg agttaaaaga aaggttttgg agtttagcta cccagatagt
     5101 agaaccaatg atagaacttg gtaaaaagaa tacaacacca tcaatagaaa ggtctgtctt
     5161 gttgagaatg ggattttctt ctcttgaagt aaaacctatt ttagaagggg ttatggaaag
     5221 aggtcttata ggtaaaggag cagggcatgt agtttataaa ttggctaaat ctaaaaatat
     5281 aacggttaga gaagcaggtt tgttattagt taagggtgaa tattgggatg aagttacatg
     5341 tttatttaga gaaggggtag aatcatgtta aaggaaaata aaaaattgga tatagactat
     5401 atattaaaag atttggataa atataaacca aaacgaagag gttgggtttg gagagaacac
     5461 ttggaaaatc ttgaaatggg accttttaaa tataaagact gtacaaaacc tttaaagaag
     5521 agtgtagggc taccatcatc taagtatttc aataatatag accctcaacc aagtccagtt
     5581 attactactg aaatagcttc tggtagattt gaggatgata taagaagaat gagaatggca
     5641 gctcatcatg gagctgacca tctaatggtt atcagaactg ctggacaatc acattttgat
     5701 gggcttatag aaggaactcc acaaggtata ggtggagttc caataacaag aaaacaagta
     5761 agagctcaaa ggaaggctct tgattttata gaagaagaag ttggtagacc tattaattac
     5821 cattcatatg taagtggtgt tgctggtcct gaagtagcag taatgtttgc tgaggaagga
     5881 gtcaatggag cacatcaaga cccacaatat aatgttctgt ataggaatat aaacatggta
     5941 aggtcttttg tagatgcttg tgaggcaaag aaaataatgg cttttgccaa tatagctcaa
     6001 attgatggag cacataatgc taatgctact gctagagaag catggaaggt aatgcctgaa
     6061 cttatggttc aacacgcttt aaactctata ttttcagaaa aaataggtat agataaaagt
     6121 aatatatgct tatcaacagt tccaccaaca gcacctccag caccatgttt aaaaatagac
     6181 ttaccttacg ctgttgcact tagagagtta tttagtgatt ataagatgag agctcaaatg
     6241 aatactaaat atatggaatc atcaactaga gaagcaacag ttactcatgt attaaactta
     6301 ttaacatcag tattaactag agctgacata caatcaacta taacacctga tgaaggtaga
     6361 aatgtacctt ggcatatata caatatagaa gcatgtgata cagcaaagca agctttagtt
     6421 ggtatggatg ggcttatgga tatgatagaa cttaaagatg tgggtgaatt aagagacaaa
     6481 gcaagagaac ttaaagaaag agctgtatta tatatggaag aaatatctga agttggtgga
     6541 tattttgaat ctgtggaaca aggatttttt gttgattctg gaaattatcc agaaagaaat
     6601 ggtgatggaa tatctagaaa aataaaaggt ggagttggtg aaggtactgt ttatgaaaga
     6661 gaagaagatt atttagcacc tgtaactgct cactttggat acaataatgt agctcaatat
     6721 gatgaaaatg ctatagataa tccatcaatg ttaatagatg gatgtacatt tgaaaatcca
     6781 gacaaaataa tatatattga tgagcttgac gattttgata atgtagaaaa tagactaaaa
     6841 gaatcatatg agtacagaaa tggaactaaa attaagcctg aaatggaatg gtgtgcagat
     6901 ggaattgtaa tgataacaat gatgcttcct actgataaaa gaacagcaga gtttgcagct
     6961 ttggaatttg tcaaaaaaat gaacttacaa gaaattgagg tcataagtag agaagttatg
     7021 catgaatctg aagggactag aatagaagtg aaaggaagag ttccgtttga tatagattta
     7081 aacaatttag taataccaga agaacctaag gtactaactg atgaagaaat aagagcagat
     7141 atagaagaaa aacctatgaa aatagtttca gctacggttg gagaagatga acattcagtt
     7201 ggtttaagag aaataataga tataaaacat ggaggtattg aaaaatatgg catagaatgc
     7261 cactatctag ggacttctgt acctgtagaa aaattggtag atgcagcgat tgaactgaat
     7321 gcagatgcaa tacttgcatc tacaataata agtcatgatg atattcacta taaaaacatg
     7381 aaaaaattac acgattattg tgtagaaaaa ggtataagag ataaagtaat gattgcgtgt
     7441 ggaggaacac aagtaacacc tgaaatagct gttgaacaag gcatagatgc tggatttgga
     7501 agaaacagta aaggcataca tgtagccact ttcttagttg aaaaaagaag agaaatgagt
     7561 aataaataat ggaaaatagc aaggtttctt taaaaataga tgtgttggta gctgaaattg
     7621 gaagtactac tacagtggta aatgctttcc acgatattaa tactaacaat cctatatttt
     7681 tgggtcaagg tcaagctcca acaacagtgt ttgaaggtgg cgatgtaaga aatggcttat
     7741 caggtgctat taaagattta gctaataaat tatctgtaga tgatatagag tataatgata
     7801 tgtttgcaac ttctagtgca gctggtggat taaaaatgac tgttcatgga ttagtctatg
     7861 atatgacagt aaaagcagct aaagaagcag ctttaggggc tggagctgtt attcgtcaaa
     7921 taacatcagg tagaattaaa agaactgatt taaataaaat aaaagaaata aacccaaaca
     7981 taattttaat agctggtgga gtcgattatg gagaaagaga tacagctatt tataatgcag
     8041 aaatgatagc ttctatgaat ctaggcatac cagttatata tgctggtaat gttgaaaatc
     8101 aagaggaaat aaggttaata tttgaagata ctaattataa gctttatata actgaaaatg
     8161 tataccctaa aatagattta ttgaatatag aacctacaag aagaatcatt caaagtgtat
     8221 ttgaagaaca cataactaca gcaccaggaa tgaaatacat taaagaaatg gttaatgaaa
     8281 atattactcc aaccccagga gctgtaatgg aagcttctaa acttttatat aaaaatattg
     8341 gagatttatt aactctggat gtaggtggag caactactga tgtacattct gttacagatg
     8401 gaagtgacta tattaataaa attttggtta atcctgaacc tactgcaaag agaagtgttg
     8461 agggggattt aggtgtatat gtaaatatga agaatatagt agaagttata ggtaaagaaa
     8521 acttacaaag tgaactatct atagatattg atgcagtaat agagaattat cctccaatac
     8581 caaaatcaaa agaagaaata ttatttgttg agaggttgac aaaagaagct gttgttaaag
     8641 ctatgctaag acattcagga aaaataagaa atatctatgg aacaactgga aaagtcaaaa
     8701 ttgcagaagg gaaagattta acagaagtaa gatatatagt tggtacaggg ggagcactta
     8761 ctaggctccc tagtagaatt gaaatattag ataatatgct caaatacaat aaaaataatg
     8821 aattactatt tccaaaggaa aaaacaaaga tattaataga taatgactat attatggcat
     8881 ctatgggtgt tttatctaaa aaatatgaag aagcatctct taagctcctg ttaaaaagtt
     8941 tgaattttga ggaggaaaga ttatgtatcc taggttagaa atagatatag aaaagttaaa
     9001 gcataatgca aaattaattt cgcagatgtg tcatgaaaga aatattaaga tatcttttgt
     9061 tactaaatca ttttgtgcac aaaaagaaat agtagaagaa atatgtagtg aaggaataga
     9121 ccatatagca gattcaagaa tacaaaactt aaaaaattta caagatataa atctacctaa
     9181 gatattgatt agaatcccaa tgttaagtga attagaagaa gttatcaatt attgcgatat
     9241 tagttttaac tcagaattag gaacaataaa gaaattaaat gaattatgtg aaagtaaaaa
     9301 tattattcat aagatagttc ttatgtttga cttaggcgat ttacgagaag gatactttta
     9361 tgaagaagac ttttttagta atgtaagaga gatagtaaga ttaaaaaata ttgaaataat
     9421 tggtatagct acaaacttaa cctgttatgg agctattata ccatctaggg aaaatatagg
     9481 cagactggta agtatagcaa aaagaatgga agaaaaattt ggaataaatc tagaaatagt
     9541 atctggaggt aattcaagtt ctattcatct gttgataaat aataatatgc cagaaggtat
     9601 tactaatttg agaataggcg agtccattct acttggtaga gaaacggcat acggtgaaaa
     9661 tataaatgga acatatcaag atgcatttaa attaatatgc caagtagttg aatgtaaaga
     9721 aaaaccatca gttcctattg gagaaatagg agttgatgcc tttagaaata aacctgttta
     9781 tgaagataag ggaatcttaa aaagggcaat catagctata ggtaaacaag atataaatat
     9841 tgactcttta attccaatag acactgatat aaaaatatta ggggcaagtt cagaccacat
     9901 gatactagat gtaagtaaca caaaatatga ttataaatta ggtgacaatt tagaattttt
     9961 attaacttat gggggtatca tgtcatctag tacgagcaaa tatgtttcaa aaaaaattat
    10021 agtgtagtgt ttaaataatt aggaggaaac tattttatga aagagcgaga atgtagaaaa
    10081 gcaagtctgt ttgatgcaat gataccaatt acttgtttga tattattttt aagtctagga
    10141 gtattagtgt atggttcatc acctcatgta ccactaatag gagcagcagc agtagcagga
    10201 cttgtggctg tatatagact tggatttaaa tggaaagagt tagaattaag tatgtttaat
    10261 agtataaaga tggcaatgca agcaatctta atcataatta taataggcgt acttatagga
    10321 acttggatag taagtggagt agttccatgt atgatttact ggggactaaa aattttatca
    10381 ccaaatattt ttttagtagc atcaacatta gtctgtgcaa tagtatctct tgcgacaggt
    10441 tcatcttgga gcacaatggg tacagtagga gtagctcttt taggcattgg tcaaagttta
    10501 ggtatgccaa ttggattaat cgtaggttct ataatatctg gagcttactt tggagataaa
    10561 ctatcacctt tatctgaaac gactaactta gctccagcaa tggcaggaac agatttgttt
    10621 acacatataa aatatatgct atattcgact ataccatcat tattaatttg tttagtaatt
    10681 tatggtgtaa ttggaatgaa gtattcaggt caagcactag atataaaaca aatagaatta
    10741 attaggtcta cattagattc tacatttaat actttatcac cagtattatt attagctcca
    10801 gcaatagtaa ttggattggt tgtatttaaa gttcctgcta taccagggct tatagtaggt
    10861 gctgtattgg gagtattgtt tgcagtagtt tttcaaggag aatctatgaa tacgatatta
    10921 gatgcagcac aaaatggata tgtttcgtca acacaaatac aggaggtaga tgctttatta
    10981 tctaaaggtg ggattatgaa tatgatgtcc actgtatcct taacaatatg cgctttatct
    11041 cttggaggta ttttagaaaa aacaggtatg cttgaagtag tagcatcttc tttactaaga
    11101 ttagcaaagg gtgtatttgg aacagtatta tgtactatgg taacttgtac aattaccaat
    11161 ataatagcag gtgaacaata tctatctata gttataccag gaagaatgta taataaggaa
    11221 tataaaaaga gaggtataca tcctaagatg ttgtcgagag ctttagaaga tagtggaact
    11281 ttaacctctc cattagtacc atggaataca tgcggggcgt atataacagc aacattaggt
    11341 gtatctgcac taacatatgg tccatacgct ttattaaata ttataaatcc aatagtatct
    11401 ttagtattaa ttgcatgtaa gtttaaaata gcaagaatag aagatgaacc tgaaacttgt
    11461 ttaaattttg atgtttgata atagataact tatgtaagaa cgtaggaact aggggggtag
    11521 cctgtgggga attagtaaga catattttag tgtgcgaaat tctataaatc agtaatcttg
    11581 tgactttagc catgggaggt tcagattagt aaatttatag ttttatatta gtctatattt
    11641 tgatctttta attgtttgta taaatttttt atagaaaaag atttctttta gacttgccta
    11701 aaaattaggc aagtctttta agtatgttct gattataaga gcactgtgtg acaagaatga
    11761 tgagattatt tttgctatac tttcgatata agaaatggaa ggaggttatg ttttatattc
    11821 atatgaaata attagttaca cagtaaattt tcgtataatt tggtataaaa agattagaaa
    11881 tgaaaataaa gtaatggagg aataaaatga ctagaatatc caatataaca atagctgaac
    11941 aatctgagta ctgttttttg gcaatacgaa aaacaattga ctttatggta gagttttcag
    12001 agttttcaaa acaaagtttt gaaaaaattt ctaaatatct ggaggagaga ggaattttat
    12061 cttcaggagc accgattgta tgttttcata acatggattt agcaaaattg gatgttgaag
    12121 tagggtttcc tgtagccact tatatagatg ggaaaaatga gattctacaa aggattgttc
    12181 ctgcacagaa aataataact gcaattgatt taggacctta tgaattacaa gaccctactt
    12241 tagaagactt attttcatgg gcaaatagca atggatataa acttcatggc gatatatatt
    12301 accagtattt aaatgatatc aatcgtccag ctagtgaata tcttacgaaa atgatgttac
    12361 ccattatata gaaagttaaa gattatcgtt tgttagttga taattagaag aacatttact
    12421 gctactacat aaattttatc ctaaataata tcaaggtgat ttaatttatg tattctaatg
    12481 taaccccaat atattgttaa ggtctactta agatgctcaa aaggctatgc ttaatttatt
    12541 gttatataat ttgtagagtg gtataattta gaggtaatat tatatagata tataggggga
    12601 aaatatgaaa atatatgata tattaacacg tttttatatt caagatatag aaaaagccat
    12661 acctttttat gaaaatcttt taaaagaaaa atgttctttg cgtttttcct ataaagaagt
    12721 tgggttggaa ttagcgcaaa taggaaatgt tttactatta tctggttctg atgatgctct
    12781 taaaccattt atagaaacaa aatctacttt tatggttgat tctgttgatg aatggagaag
    12841 ttatctattg gataatggag cagttgtagt aagagataag aaaaaagttc ctacaggata
    12901 taatatgaca ttgagacatc cagatggtac aattatagaa tatgttcaac atacaaagca
    12961 ggattagtta aaaacagatt ttactactga gcaactaatt agaatctatt cttagacgta
    13021 caatttatta tttttggtat taacagatta taagtcaaat tataatattt caaaatagaa
    13081 tagaaattta tttacataaa agagagtgtt taaaaatgaa ctttttagtt catgaagcac
    13141 tcttttttgt atgaaatcaa atacatcaat atataaaata aactgatgaa gagcttaaat
    13201 taaaataaaa agattcataa caggacccca ttaaacttta agatagggta agtttgtaat
    13261 catattaaat aattgtaaaa agctgttttt agtcgtataa tataaatatt tatcatttaa
    13321 ttaaggaata taaaaggaat ctaataagaa gaaaataata aataactttt tagatttact
    13381 aaatgaaaaa ttattgaaaa ttaaaggatt agagtcttta atcagaaagt aaaaagtaat
    13441 atattaaatt tatgaattga aaacttaaac aacaaatttg tttataaaat agcaagattc
    13501 attaaatata actaagtaat aattgatata atgttgcatg gataaaagga gggaagctgc
    13561 aatgtcttac aaagtaatta atgtcataac tgctattgac tatatagagg agcatttatc
    13621 agaaaagctg gatttagata tagttgctaa tgctgttcat tactcaaaat atcacctgca
    13681 tagaactttc acaacttctg ttggcctaac aatgcatgat tacataaagc ggagaaagtt
    13741 gacagaagca gcaaaattat tggcattttc aaagaaacca attattgaaa ttgcattgat
    13801 agctggatat gaaagtcaac aggcattcac ttctatattt aaagctatgt ataaaaaatc
    13861 tccaaataaa tataggaaag aacaagaatt ttatcctttg cagctaagat ttgttttgaa
    13921 aaaagataac ctattttcaa agaatgtact agagttggaa aaagaaatta aacttgcaag
    13981 tatgtctgac ataccattat ggatgaattt agtgagatta gtcatagatg gatttccaaa
    14041 tttacaagag gaagaatata tatatcaatt agaacagtat attttagaag aaagagcttt
    14101 aatattaaaa cttaataatg tcgctattgc aaatatggtg tttaatagag aaactagaag
    14161 tattgatttt tttggaatac atccacagta tcgtaattca gacattgcac aagtgttttt
    14221 gaagaaagtt attgaagatt tcttgataga tacagatatt tcaattacta cctttcgaga
    14281 gggtgacaaa gctgatacag gacatagaga tatgataaag aagttgggct ttgcagaagc
    14341 agagttgctt gtggaatttg gatatcctac tcaaaaattt attcttcctt taagttaaat
    14401 agcaagaatg attaaaaact ccaaaacaca attaagtata atgattcagt gtaagtactt
    14461 aattaacaaa ggaggattta aatgtctgaa aataaacaag tagtaaattg cgatattatt
    14521 gcatctaatt ctaaacataa tatgtgtgaa tatgatgcag agttattaac tttttttcca
    14581 gatgagattg cacatctaat agagataaat aacaagctag acaatgcttt taagaaagct
    14641 gaaaatcttg ttgacaagct tgataaggat tatatggacg ctaaaatgta tatggtaaaa
    14701 aatcgtggtg aaatagaccc acatgaaatg tttcaaaatg aacaaggact aaaacaaata
    14761 gataactatg gggcttttat ggtaaaagtt cgagataaga ttaataaaat caaagattct
    14821 ccatactttg ctagaataga ttttaggctg aaagatatgg atgatgaatc taagtattat
    14881 attggtagat ttgcatttga ttatgaagat gagcttataa tacttgactg gcgttctcca
    14941 attgccagta tgttttatga ttatgaaatc ggaaaagctg gatatgatgc tccaatagga
    15001 tgggtggatg gagaaataac aagaaaacga caatttaaaa ttaaaaatgg aaaattagaa
    15061 tatgcactag aaagttccat taatatacaa gatgatattc ttcaaaaaga acttagtcac
    15121 acttcagatg aaaaaatgaa gtcaatcatt tcaacaatac aaaaggaaca aaatcaaatt
    15181 attagaaatg ataaagctga tacactgatt attcaagggg tagctggttc tgggaaaaca
    15241 tctatagcat tacacagaat tgcttttctt ctttatcgat ttaaagataa aatatcagca
    15301 aataatgtaa ttatcctatc acctaataaa gtctttggag actatatttc caatgtcctt
    15361 cctgagcttg gagaagagcc attatgtgaa ttaagttttg aaaatatagc agaggtacaa
    15421 ttagatagag ttattaattt tgaaagtgag aaagacccat tagaaataaa tgatgctaaa
    15481 tggtctgaaa gagtgcgttt taaatctaca cttgattttg taaaacttat tgatgattat
    15541 ataaaacaaa tgccaaataa gatttttatt ccaaaagact acacttttgg aagttttaca
    15601 gctaaaagtg actggataca gagtagattt gaggcatata acagatatcc agttaagaag
    15661 agattagaga aggtagctga agacattcat tataaatttg aatcagataa tattatggaa
    15721 gaagatttac caaaggtaaa aagtatactt aaaagtttga atggaatgtt gacaataaaa
    15781 aatactctta cactatataa ggatttcttt aaacaaatga atatatcaaa catgtttgtg
    15841 atggcaacaa aaaaaactct tgaatggtca gatgtatacc catttatata tattcatgct
    15901 gcatatgaag gtatacaaga agataagatt ataagacatg ttgttattga tgaaatgcag
    15961 gattacacac ctattcaata tgctgtaata aacttacttt ttaagtgtaa aaagactatt
    16021 cttggagatt ttggacaatt ggttaatcct aatcacactc atacactaga tgatatgaaa
    16081 cagctatata atgatgggga actagttatg ttgaataaga gttaccgttc tacttttgag
    16141 attattaatt tcgccaaaaa gattcaagac atatcttcat tagagcctat tgaacgacat
    16201 ggtgaagaac cagctttatt aaaatgtaat aacgagcaag atgaaataaa taagataaaa
    16261 atagaaattg aagaatttaa aaagagtgat aatgcgactc ttggtattat attaaagaca
    16321 gataatgacg ctaaagctgt atatgatgcg ttaagagaaa aatatagtgt tcacttaata
    16381 tcttctgaaa gttctagctt tacaaaagga gtatccataa cttctattaa aatgtcaaaa
    16441 ggtttagagt ttgatgaagt tattgtacct tcagtaaata ataaaacata ttatagtgat
    16501 tatgaccgca gtttgttata tattgcttgt acacgagcta tgcataaact taagttgaca
    16561 tatactggtg aattgactca acttatagat atgtagatta tatacttatt atgtaagtgt
    16621 ttacttatat aaatataacc tgtcaataaa attcattaat aaatcaataa aaacaatatt
    16681 tgtaaaagta aaagctataa attatatatt aaattttata atttatagct tttataatac
    16741 tgcttatctt aatcctccca atagcaaaat aattttaaac atgccatatt ttataaatgt
    16801 aataacaatt atacaattaa aataactttc ccaaactaac ttgacattta aaacttacta
    16861 ttgtaatatt taaatattac aatggtaagt tttaaatgtg gaatcaatta ttatatcaca
    16921 aattaaaagg aggacaggat gttattaata gttacttaat tatcttcgca ataggtttaa
    16981 tcaacataat tgtactaggt gaagaaaata taagagatac agctattcat ttagtcttgt
    17041 ttcttataag tagaacctag attggagtat attacaaata atgtctacta ttttaatagt
    17101 aggttataaa tttaaatatg ggagatgata taaattatga aaagaaaaaa gaattttatt
    17161 tggatagcta tcttattggt tggtgtagtt atggtaatgt attatgtagg gaaaaaacat
    17221 aacgatataa atcaaaaaac tgataagaat tatataaaat ctgaattaaa taagtctgaa
    17281 ttaaataaat ccaaaattga aaacaataat aaaaacaaaa aagaaaaaat taatatggtt
    17341 gactatagtg attgttttga aggtattagt ggaggagcta ttttttataa tactaaaaat
    17401 aaagaatata atatatataa caaagaatta attgaaacaa gaaggtcacc atgttctact
    17461 tttaaaatag tttcaacttt aattggttta gaaaaagggg taataaactc aaaagaatca
    17521 gttatgggat atgatggtac agagtatcca aataaaaatt ggaataaaaa tcttagttta
    17581 gaagaggcat ttaaggagtc ttgtgtttgg tactacaaaa aattgataga taaagttgat
    17641 gctaaatctg tccaaaacat tttagatgat ttaaagtatg ggaattgcga tattagcgaa
    17701 tgggaaggag atttaaaaaa tggaaaggga catttaaatg gattttggtt agaatcttca
    17761 ttacaaatat ctccaaaaga acaagtgcaa acaatggcaa aaatatttga gggagataca
    17821 aactttaaaa aagagcatat taatatttta agagatatta tgaaaataga tgtaaatgat
    17881 aaaaacataa atgtatatgg aaaaactgga acaggatttg atgaaaaaaa taaacgtgtg
    17941 gatgcatggt ttgttggtat gctagagcgt gaaggtgaca cctattattt tgctattaaa
    18001 tcagatgatt ccaataaaga aattacaggg ccaaaggtaa aggaaattgc aattaatata
    18061 attaagaaat attatagtgt aagggaataa acttaatttt atttgtaaat attatttacc
    18121 tatagtctgt aatatttatt aaataatttt actaaattga gtctatatag agcaaaactc
    18181 ctttagtaca taaaatactt gctattgatt attaaattga atacctatag atttaaagtt
    18241 aatacctttt aaagactatg taatatatta aaagtataaa taaaatatat tgcatagttt
    18301 ttgttttata ttataatttt aaataatgtg caaaaactaa acaatatttt taatagtaat
    18361 ttatatttgt attcatgtca ctataatgtc attttaggtt gctaaaattt aaccataaag
    18421 gagatgattg tatatggaaa tattaaaatg tgaaaatctc acaaaaatat atggttcaaa
    18481 tcaaaccaga gtgacagccc ttaataatgt aaatttatca gttcaaaaag gtgattttgt
    18541 atccattgta ggagcatctg gttctggcaa atctacattg ttacatctgc ttgggggagt
    18601 agatagacct acatctggaa agatttatgt tgaagataca gaaatatcaa gtttaaagga
    18661 agaagctctt gcagttttta gacgtagaaa agtaggtctt atttatcaat tttataatct
    18721 tatacctacc cttgatgtta gaaaaaatat attattaccc atgttacttg ataaaagaaa
    18781 ggtggatgag gatagattct ctgagattgt atctatattg ggattatctg atagattaaa
    18841 ccatcttcca agtcaacttt caggtggaca acaacaaagg gtatctatag ctagaagttt
    18901 aatctataga ccagctattt tattagcaga tgagccaact ggtaatcttg atagaaaaaa
    18961 ttcagaagaa attgtagact tgttaaactt atcaaataaa cgatttaatc agacaatact
    19021 tcttattact catgatgaga aaatagcact agaggcaaat cgtattgtaa ctatggaaga
    19081 tggagtgatt gtctctgaaa aggtggtgaa gaaatgaata ttcttcaaga atacacctta
    19141 gactttgtac gacgcaataa acgtagtagt atagctataa tgttagctat attacttact
    19201 acaaccatga tgtcttcttt gtgtggactg ttgtatacca tgtggacaga ttttataaaa
    19261 ctttcaattg agaaagaagg taactggcat ggtgaattgt ttgactatac ttatggaagt
    19321 gacttaccat taattgaaaa tttttcttca gttgatgatg taatggtaaa aggacaatgg
    19381 tatgtaggca aaattaatga tgagaaaaga gattatataa tttatagaaa tgctacatca
    19441 gagtattgga attctatgcc agaaaaggat actataatag agggtcgtcg accaaataat
    19501 gctaatgaaa ttgcattatc aaaacaatat tttgagaata atccaagtgt aaaaattggt
    19561 gataaaatta ctctcccaat aggagataga atcctaaatg gaaagcctct tgaacccata
    19621 gccccaaaat cgaaagggga aagttttaaa aaaatttcag aggctacatt gacaatagta
    19681 ggaaaggtag atgtttctac ttcatcagta atacctgcat atacagctct tggatatctt
    19741 gataaaacaa gtataaaacc taatgatagt ataactgtgt acttgcgatt taaaaatata
    19801 agagacacgt ataaggaact tccaaaactt gctaaatcat tgggatggaa gactaatgaa
    19861 tatggaaaat acaatcttaa atacaatagt aattatcttt taaagatgtt agtcttttca
    19921 ccagaacaga aagcatctat gagtagtata tcaaaatttt caactcctat tatgtatctt
    19981 acaatagcaa tttttacagt agccgtgttt gtaatggtga tttacaacgc gttttcactg
    20041 tctgcaaatg ctagacttac tcaattaggg atattatcta gtgttggtgc atcaccaaaa
    20101 caaattaaaa ggtctgtagt gtttgaaggc tttttactga caattatacc attaccaata
    20161 ggtttatttt taggatggtt attgtgtaat aggcttatag tttatgttaa ttcagttaat
    20221 tatcatactg atgttccaga agtggtattt acttatggta ttcctgcttt tctgccagca
    20281 gttttactta caatagtaac agtctggatt tctgcactta taccagcacg aaaagtatca
    20341 aagatatctc ctatagaagc aatacgacaa ggagatagtg ttaaaatcaa aaagtcgcat
    20401 aaatattcat taggaaagat atttggaata gaaggagaac ttgcatcaaa cgcactacgt
    20461 gctcgtaaaa aatcttatcg tactgcaaca atatctttaa cattatcttt tttattactt
    20521 acatgttttt tgcatataat tgcaaatcaa gaaggagcaa aagctgttta tggaagtgat
    20581 aattataagg accaaagaga tatagcagtt tatttggaaa atagtgaacc tgtagagtct
    20641 gctttcattg aaaaattaga taatacagaa ggtgtaaaaa gttcactata ttatcaaaaa
    20701 atgtctgctt ccacttggtt gactgaaaaa gatgcttcaa aagaacttgc gaaaaatgga
    20761 ggatttaaaa atatagcttc atctaaaaag tactctccta tagaacgtga tggcaagttt
    20821 cgtataattt caaatataat tgggcttgat gataagagtt ttacagaata ttgtaggcaa
    20881 atttctgtag acccaaagtt attttatgat acaaaaaatc caacctcaat tgtatataat
    20941 aaggaggaag atattaatcg tagtacacgt agaaataaag tatatataga ttatttaaat
    21001 ctttcaatag gagataacat taaattaaat gaaaaagtat atgacgaaga taaaggggat
    21061 tatacttaca acatgaaagt gggagctatt acaagcacac ttcctaaaat agtagatact
    21121 agttcaaagt atacattaat gcaaatagtt ccaatgtcta tggtgcagga aatatctaaa
    21181 aattatgatg aagtaagtag gttacgttca aataggctga taggagtatt taaagtaaat
    21241 aatcgtgagg acattcctag agtaagagaa aaattagagt ccatttataa taaagactat
    21301 ggttcaggag attatagtat tgaggatgtt ttagaaagtg aaaaacaaaa ggctagtgga
    21361 ataaagacca tgaatttaat agtaggcttt attacaggtt tattggctat gattggatta
    21421 tcaaatgtac ttgctacagt gtcaggaaat attagaagta gacgacaaga gtttgctatg
    21481 ttgcgttctg ttggattatc tccagaaggc ataaaaaaga tgcttatttt ggaaggttta
    21541 ttcttaggaa ttacaccatt actacttagt attcctgtgc aaataggaat agtgtatgct
    21601 tttctacgta taaatgaaat atattttata gagtatttac catttgcacc tatatctaca
    21661 ataataggtt ttactatatt gatacttttt attgtaatag catcatatat gacaggatac
    21721 aagcagttga agaatgaaaa tatagtagaa tctataaaaa atgaaacaat ataaacaaaa
    21781 tctatttgaa cttatttaaa ggtttcgatt taagactact tacaagtata gggatagtta
    21841 agttgtttgt ttaataaaac cttaaatgta aatagaagta tattactaaa acaatagata
    21901 acatagaata tagatatagc tggattatag gtattttacc tataatccag cattttatgt
    21961 gtaaaattta attatgaatc ttgcaccacc attatggcaa ttttctgcat gtagagtacc
    22021 attttgacgt tctataatag actttgatag agcaagccct ataccaatac tgtccttact
    22081 agcattttta ccacgataaa atctattaaa aatatatgga agttcttcct gagcaaaccc
    22141 cttaccatta tcttctatga taatctcaac atatagagga ttttgactat aataagcaac
    22201 tatatatcca ccatcttcta tatgctcact acaattttta agaatattta aaagagcttc
    22261 taaagtccaa ttaatatcga tttcaaattc taccgaagga gaagcctcga tagtaaatgt
    22321 ctgatgtttt tctcttaaag aaccttctat aggctcaatt gcataagtaa gaagagaatg
    22381 tacatctata agtttgcttt caaatattaa agtacttgca tccaattttg ataatgtaag
    22441 taaagatgaa actagtctct caagtcttga gatttgattt gtaagtctgt taatataatc
    22501 tatttcttca tctggacaat tttctgaaag taattgtgcc ataagtgaca tagaggtaat
    22561 tggagtctta agttgatgag caatatctgc aagattatct gaaagagttt gtctttcttt
    22621 taaagcaaat tcttttgaac aacgaagttc cccaactgtt ttatatatct catcttcaag
    22681 gtgagaaaat tcatcttcac atcgtaaaag tacagactct tttcctaaat tcacttcttc
    22741 taagtattga gtaagattgt ttattctact cagtttttgc ttatttgttc tatgtttgat
    22801 tatatatata actgtagcca ttgttgaaat tataccaaac gatatcacta gaaaataaaa
    22861 aaagttttta ctccaaaatg tacttttaga atatccatgt tttgataaaa tttcatttcc
    22921 tgttttaaaa tccttatcac tagaattttt cagtgatttt agtatacttt ctgtagactt
    22981 aggattgtac tcatatgatg caccaagagc cttagacagt acattgtatt cattaaaaga
    23041 agttatagtt gttagaatta cactcaataa aaaagttaca attaagcaga atattataaa
    23101 aacaactgta ctgttgatat ttcttggagt tttcactagc tgtcctccat tctatatcct
    23161 atgcctctta atgtttttat acaaggtgga ttttttaatt tttctctaag acgctttatt
    23221 gtaacagtaa gagtgttatc atttacgaaa ttaccatcta catcccagag catttctagt
    23281 aactttgtac gagtcatagc tctattttta ttttccataa gacttagaag aagtttatat
    23341 tctcctgctg tcaaaggtac ttcaacatta tctaaatatg ctttgacttc aaccttattc
    23401 aagcttatat tttcacatgt aatagtattg ttagtatttg aatgagttct tctaagaact
    23461 gcattaattc tggattgaag tactgagagc aaaaacggtt taaccacata gtcatctgca
    23521 cccatatcta atccacatat aatgtctttt tcatcatcac ggactgtcaa aaatatcact
    23581 ggagtatctt ctatactttt aacatattgg caaaactcgt atccaatacc atcaggtaga
    23641 tttaaatcaa gcagaattaa atcaatagtt ttatcaaaat tttttttagc ttcagcaatc
    23701 gtattataac aacatacagt gtgatttttc ttttcaaggg aagtttttat tccaaatgta
    23761 attgtatcat catcttctaa aagaagtatt ttagccattt acttttaatc ctttcagtaa
    23821 tttagttatt ccattaacta agttattagt tttatgttta atatttttta tattagagat
    23881 agaaaaaatt atcatttaag tatataaact ttaatttata aactttaatt tataaactta
    23941 ggtttatgct ttaattgaat ctattaaaag agcaagagtt gtttcaataa atttttcctc
    24001 atctatatta aagttgttca ggtatgattt tcctgttaca gatagtaaat taaaaaatcc
    24061 tgtaagtgta aatattgatg aatacataag agtaggtatt tcaatatcat gcctaatact
    24121 tctatcattt tttccaattt caaacattcc ttgtatttca tcaaacataa atttattaaa
    24181 agaaaaaaac ttttctttaa acagcatatc agtatccgat ttttcagagt ttacaatacc
    24241 aatcattccc ataaggcata atagagaagg ttctacagag taaaatttat gaaaagcaaa
    24301 gtatgaaagt tttatttttt caaaccctgt tttgcctttt gcactttcta ttttaacatt
    24361 atcccaaagt cttttatatc ctcttaagac aacagcaaag aataaatctt ctttacatga
    24421 aaagtacttg tatatggtgc gtttagtata ttcaacttct tttgctaatt cattcatagt
    24481 tgtattatca aatccattaa gacaaaataa tttttctgcc ttatcaataa tctcattttc
    24541 tctttgtatt ttttctttat ctctttttga taattctgac atataaatta ttcctttcta
    24601 ttgataaaaa agtatactga tggtatattt atgtaaatat aatatttgta tacctatagt
    24661 atactttaat aaaaaataaa attaatatca agtattataa ctatttataa ctttattaat
    24721 taattatata ggtaaaatac caaataaggg gaatttattt atgaagataa aagaaaatgt
    24781 attaatgtta gatagtactc gtggctcaaa ttgttttatt gtgtttgata aagaaattat
    24841 tcttatagac acaggtcttc cttttatggg gaaaaatata attaaagaat taaaaacact
    24901 tggcattaaa cttacagata ttaagcatat tttattaact catcatgatg ttgaccatat
    24961 atctaatatt aaattattga aagaaaaaac aggagcaaaa gtatgggctc atgtagagga
    25021 tatacctttt attataggag agaaggatag acctgggttt aagaagttta ttggaaaggc
    25081 aatttccaga aggctcatta aggatgttga accttatgga gagaatatgc agattgggaa
    25141 tataaaaatt atccatactc ctggtcatac tccagggcat gtctgcatga tttttgaaga
    25201 tgtattattt gcaggtgatt tagtcaaaaa taagaatgga aatatagttc cttatcctaa
    25261 tccatggaat tgggattata aaaagatgat gaaatctata agagaattgg ataatttaaa
    25321 atatgaatgg atatgtatgt ctcatggaac tccttgtatt aaataataaa aattaacatg
    25381 gattaataga ggcgtagttg cttgtatgta tgataataat ttacagcttc ttttt
//
LOCUS       DAGSHT010000035        24556 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGSHT010000035 DAGSHT010000000
VERSION     DAGSHT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.036"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="MJB72_001321"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="MJB72_001321"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="MJB72_001322"
     CDS             275..466
                     /locus_tag="MJB72_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBY8262445.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="MJB72_001323"
     CDS             621..2030
                     /locus_tag="MJB72_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY8262446.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="MJB72_001324"
     CDS             2161..2850
                     /locus_tag="MJB72_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBY8262447.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="MJB72_001325"
     CDS             2853..3788
                     /locus_tag="MJB72_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBY8262448.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="MJB72_001326"
     CDS             3785..4678
                     /locus_tag="MJB72_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBY8262449.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="MJB72_001327"
     CDS             4756..5502
                     /locus_tag="MJB72_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBY8262450.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="MJB72_001328"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="MJB72_001328"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBY8262451.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="MJB72_001329"
     CDS             6359..7063
                     /locus_tag="MJB72_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBY8262452.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="MJB72_001330"
     CDS             complement(7251..7679)
                     /locus_tag="MJB72_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBY8262453.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="MJB72_001331"
     CDS             complement(7716..9146)
                     /locus_tag="MJB72_001331"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBY8262454.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="MJB72_001332"
     CDS             9278..9862
                     /locus_tag="MJB72_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBY8262455.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="MJB72_001333"
     CDS             9878..10399
                     /locus_tag="MJB72_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HBY8262456.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="MJB72_001334"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="MJB72_001334"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBY8262457.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="MJB72_001335"
     CDS             12762..13532
                     /locus_tag="MJB72_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBY8262458.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="MJB72_001336"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="MJB72_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBY8262459.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="MJB72_001337"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="MJB72_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBY8262460.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="MJB72_001338"
     CDS             14894..16009
                     /locus_tag="MJB72_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBY8262461.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="MJB72_001339"
     CDS             15999..16868
                     /locus_tag="MJB72_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBY8262462.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="MJB72_001340"
     CDS             16861..17727
                     /locus_tag="MJB72_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8262463.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="MJB72_001341"
     CDS             17768..18481
                     /locus_tag="MJB72_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8262464.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="MJB72_001342"
     CDS             18482..19651
                     /locus_tag="MJB72_001342"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBY8262465.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="MJB72_001343"
     CDS             19655..20491
                     /locus_tag="MJB72_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBY8262466.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="MJB72_001344"
     CDS             20492..21487
                     /locus_tag="MJB72_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8262467.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="MJB72_001345"
     CDS             21505..23193
                     /locus_tag="MJB72_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBY8262468.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="MJB72_001346"
     CDS             23205..24272
                     /locus_tag="MJB72_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8262469.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9802 a         2752 c         4384 g         7618 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggtcttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagcgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaagtaag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggatacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gaattttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc tagctcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagata ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaaa ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atacataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaactta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    24541 aaactaaagt aaataa
//
LOCUS       DAGSHT010000036        23695 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGSHT010000036 DAGSHT010000000
VERSION     DAGSHT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.037"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            68..141
                     /locus_tag="MJB72_001347"
     tRNA            68..141
                     /locus_tag="MJB72_001347"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="MJB72_001348"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="MJB72_001348"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="MJB72_001349"
     CDS             540..2558
                     /locus_tag="MJB72_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8262470.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3063..5075
                     /locus_tag="MJB72_001350"
     CDS             3063..5075
                     /locus_tag="MJB72_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8262471.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5317..7230
                     /locus_tag="MJB72_001351"
     CDS             5317..7230
                     /locus_tag="MJB72_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262472.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDNLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRVEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNLKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            7223..9442
                     /locus_tag="MJB72_001352"
     CDS             7223..9442
                     /locus_tag="MJB72_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY8262473.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQQPCIYLSRLNQHIIEKRSN"
     gene            9551..10381
                     /locus_tag="MJB72_001353"
     CDS             9551..10381
                     /locus_tag="MJB72_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBY8262474.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKIVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIIYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10371..13838
                     /gene="addB"
                     /locus_tag="MJB72_001354"
     CDS             10371..13838
                     /gene="addB"
                     /locus_tag="MJB72_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBY8262475.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESTKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13838..17665
                     /gene="addA"
                     /locus_tag="MJB72_001355"
     CDS             13838..17665
                     /gene="addA"
                     /locus_tag="MJB72_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBY8262476.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTVVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17711..18922
                     /locus_tag="MJB72_001356"
     CDS             17711..18922
                     /locus_tag="MJB72_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBY8262477.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18909..22436
                     /locus_tag="MJB72_001357"
     CDS             18909..22436
                     /locus_tag="MJB72_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8262478.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKIKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKNLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22567..>23695
                     /locus_tag="MJB72_001358"
     CDS             22567..>23695
                     /locus_tag="MJB72_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBY8262479.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIG"
BASE COUNT        10173 a         2319 c         4178 g         7025 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattatta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg tacttattta taattttggg taaaaacact ttgatataat
      481 ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc gtcgcaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagca aatcaaaagt cagtgttaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctctaaatta taatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cacaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgcgcca atacttttgg
     1201 ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctagag
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtatacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaacacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggtc ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagtta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccatta gctgccaact
     1801 taaaatctcc aatacttata acaccaaaga cgtatgtatc tgcataccat aaagaaaact
     1861 tagaagctaa atcagctaat aaagtatata aaataggagg aggattgact tctaaggtaa
     1921 tgaactctat agcatcatca ttatctaaac acaatacaac tccaacagaa ccaggaaata
     1981 gtgggggtaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt aaagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagacaca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg aggaactact aagactgtag ctacaaatat attaaataga atcttagaga
     2341 aatttaatct aaaaaataga ggtgcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tggataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gcatagaaaa
     2581 atttaaaata aattaatcta taattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataatag taataaagac tgtatataca ttgtgcatat aaatgcgaag tgatgtatat
     2701 acagtctttt tatgattaat tgtaaaagga tttatgttat atgaagaatt tacatatatt
     2761 aaggctacag ttaattgtca agagctggag cattcacttg taatacacaa taaaagttta
     2821 tttactatat ttgattgtgt gtttttgtgt tttttgtgga tttttatggt aataaatgat
     2881 atagtataga taattaaatg tattaaatat atttaatttt tttatttttt gagttttata
     2941 agtttatttt tatatattac ttatatatta tttatatttt gggttagata cactttgaca
     3001 tattttcaaa agttaaaaga ttaaaaataa agtagaaatt tatgttaagt aaggagatta
     3061 atatgagaag aaatacaaaa ttattaacaa cagggattct ttcaatggca atcgtcgcac
     3121 ctacaatggc atttgctact gaatctaatg ctatggaaaa taacgctgat ttaaatataa
     3181 acttagagaa aaaaagtatc gttttaggta gcaaatcaaa agttagtgtc aaatttaaag
     3241 aaaaaccaga tgcagatagc attacattaa agtataaatg ctatgacatg ccattggata
     3301 caactctaaa ttacaatcaa tcaactgggg catatgaagg aactatcaat tataaccaag
     3361 acccagaata tctaaatgtt tgggaactac aagggataac aataaacagc aaaaataatc
     3421 ctaaaacttt aaacagacaa gacctagaaa agctgggatt aaatttaaaa gactataatg
     3481 taacacagga atgtataatt gaagacataa cttctagaaa agatgtaaat aaatatttga
     3541 gaaaaacttc ttcacctatt acagaactta caggaagtga tagatatgaa acagcagtta
     3601 aaataagtaa agagggttgg aaaaatggtt cagataaagt agttataata aatggggatg
     3661 taagtataga tggcattata tcaactccac tggcaaccac atataatgct ccaatacttt
     3721 tggttgaaaa aaacaatgtg cctaatagtg taaaatcaga attaaagcgc ctaaacccta
     3781 aagatataat tataattgga gatgagaatg ctatttctaa aactactgct aatcaaatta
     3841 aatcaactgt aaatgctagt caaacacgtt taaatggttc taatagatat gagacatctt
     3901 tattgatagc aaaggaaata gataaaaatc atgatgtgga aaaaatatac ataacaaatg
     3961 ctaatggcgg agaagtggat gcacttacta tagcagcaaa agcaggtcaa gacaagcaac
     4021 caattatatt aactgataaa gatagtatta cagacaatac atataaatgg ttaaagagtg
     4081 aggatttaca aaatgcttat tttataggtg gtcctcaaat gatatcaaca aatgttataa
     4141 ataaggtaaa tggaataact aaggatagtg ttactaataa tagagtatac ggagcagata
     4201 gacacgaaac aaatgcaaac gtaataaaaa aattctatac agatgatgag ttagaggctg
     4261 ttttagtagc taaatcagat gtacttgttg atgctttagc agcaggtcca ttggctgcga
     4321 acttaaaatc tccaatactt ataacaccaa agacgtatgt atctgcatac cataaagata
     4381 atttagaagc taaatcagct aataaagtat acaaaatagg aggaggattg acttctaagg
     4441 taatgagctc tatagcatca tcattatcta aacacaatac gactccaaca gaaccaggaa
     4501 atagtggggg caagacagtt atgattgacc cagggcatgg tggttcagca cctggaaatt
     4561 catctggagg aatgattgaa aaagattaca atttaaatac ttcacttgca acaactgaat
     4621 atttacgttc aaagggattc aatgtaataa tgacaagaga cacagataag actttatctc
     4681 ttggaaatag aactgctcta tctaattcat tgaaaccaga tttatttaca agtatacatt
     4741 ataatggctc aactaataaa caaggtcatg gtgtagaagt attttataag cttaaagata
     4801 aaaatggggg gactactaaa actgtagcta ccaatatatt aaatagaatt ttagagaaat
     4861 ttaaacttac aaatagaggt ataaaaacaa gagtacttcc tagtgattct acaaaagatt
     4921 atttatacgt tttaagaagt aatgatatgc cagctgtact tgtagaatgt gcatttttgg
     4981 ataatgaaaa tgatatgagt ttaataaact catctgcaaa agtaaaagaa atgggtacac
     5041 aaataggtaa gggaatagaa gattcattaa aataaatgga aataaaaata tttaaaaact
     5101 taatgtaaat ttatagttgt aaatttatac taaagtcaaa aaatgaaata taagtgtatc
     5161 aatagtaaga ctgtatgtac atttgcacat gtgaatgcaa gtatatatac agtctttttt
     5221 tgtagttaaa tgcaataatt tgaatgtgtt ttgtaaaata cacaatatga tataattaga
     5281 taatatacac ttaatttaat tgaggaaggt gattatatgg aatatttatt tgaaaaaagt
     5341 agtttaaaag aatatggaaa tgaagcttta gataaatata gagaattatt agataaaact
     5401 ggtaataatt caaggtgtat tcttttgtta gtaccaaaca ataacacaaa gatacgatat
     5461 gaaagggctc taagattaaa ttatagtgaa gaactgaaaa taacaacata tataagtttt
     5521 gtgaagaaag aattagttaa atactggcca ctaataatag aaaaatgtga tgaaataagt
     5581 acaaaggttg tatctccaac atttatatca aatagtttaa gtgaatattt aatagtgcaa
     5641 aaagttaagg aaaaaaggat acaagaagga tattttgaag atattacagg aaccaataaa
     5701 tccatagcaa atagtataat gactaacata aataagtctg catttaattt aattgatttt
     5761 gattttattg gagaaaaaat atattcatca aagaaaaata aagatagtat atatagattt
     5821 tcatacactc aaatggatga aattatatca tattatataa attccttact atcaaattca
     5881 attttagata atgcattaag catatattta tacaatcaat acctacttaa tgatgatttt
     5941 tatttaaaaa acttatttac agaagtgaga tatataattg tggataacct tgaaagctca
     6001 agtaatgcag aagttgattt tataacagaa gcattggata atacattaca aagttatata
     6061 tattttgatt atagtaggga ttattctgta tttaataatg tggatataga ctatataaat
     6121 gagaaaataa tatcaaaaat taagcctaaa gaagaaaata ttaaaaagaa ggagattaga
     6181 gtagaagatt tatacttatt acctgcaaat atagaattaa atcaatcaag tcaattatat
     6241 aatgaaatgt tagatttaat aagtgaaaaa gttatatctc ttatagaaga agatgtatct
     6301 ccaagagata tagcaattat atctcctata aacaatagta tattggagca tcaaattaga
     6361 gattctttga tagaaaaaaa tatagatgtt tttagtacta agaaggataa aaaagctgtg
     6421 gattatccat atgggaatgc attggtagtg gctacatgta tattctatgg atatttagat
     6481 tttataaaag atgatgaata cttaagcttt ttagaaactc ttttagaaat aaataggata
     6541 aaagcattta agatttttaa tgaaacaaga catttaagta ttgataatat agatgaacat
     6601 agtcagtatc gtgatatttt acagtatatt gaagaaaaaa agaagagtga tataaaaata
     6661 catgaatttt taactcaatt ttacatagat aaaatgttaa atttaaaaga aggtaaaaag
     6721 aatgttgggt tatgtaaaaa gataatagct gaaagtgagt cttttagtga aagtttaagt
     6781 ctacttggga tgaaaaaaga aaagatattt gtagaagctt taaaaagtac aataaacgat
     6841 tattactctg ttgtcgatat agaagagttg aaatccaaag acaaaatagt aataactact
     6901 ccttattcat atatatcatc aaatatagat agaagtatac aaatatgggt agatatagga
     6961 agtaatgctt ggaatctgaa aatagaaaaa gatataagta atttaattgt acttagaaaa
     7021 tcttttgaag aaaaaaagat ttatacaaat gaaatggaag aatattataa aaaatattat
     7081 ctatacaata cagtatataa tttgttatta aatgcaaaga aaatctatgc atttaaaagt
     7141 gaatatgcga ttaatggtta tatccaagag agtatattat atagtatact tttaaaaatc
     7201 tcacataaag gagataaaaa ttatgattaa tataaattat agagaagacc agataccaat
     7261 aataaattac gataatggta ctatggcagt accagcagta ccaggtgctg gaaaaacatt
     7321 tataataaca aatttagtag ctaaactatt acttgaacag aaacataagg gtggtaaaat
     7381 attaattctt acatatatga atagtgctgt aaataatttt aaaggtagaa taagaaagat
     7441 tcttgaagag aataaaatag atgatactaa ttcgtatgaa gtaatgacta tacacagttt
     7501 ggctgtaaaa ataataaagg aaaagccaga agttgtaatg ttaagtgagg attttaacat
     7561 agctgacgat ttgcaaaaaa gtattatatt aaatgaatgt ataagtagat ttagatttga
     7621 aggtggagaa agagctttta gatggttttt aaaggagcaa aaagatgaac gctggaaaga
     7681 aataactctt gaagcctggg aaagaggttt ttttgaattt gcaggaaatg caataagtga
     7741 acttaagtat aagggaattt ctccagataa acttgaagaa atattgtcaa gaggacacaa
     7801 aggaatgctt aaaattattt taccaatata taaattgtat gatagaaaat taaagcaaaa
     7861 tggattgcta gattatgatg atatattaat tttagcagaa aaaactttaa gcttagacga
     7921 agggcttaga aagaagtttc aaactagata caaatatata tttgaagatg agtgtcagga
     7981 ctcaaatgaa attcaaggta atataataaa aattatatct agcgaaaata aaaacctagt
     8041 tagagttgga gatataaatc agagtataac aggaacattt tcatcatcag accctaaatt
     8101 ttttaaagaa tttataaata gtgcagattt ctgttatagg atggatatgt caaatcgaag
     8161 ttcaaaagat atacttgatt tagcaaacac tcttgtcaag tatgttacaa aggaatttag
     8221 acagaaagaa tgtagacatg ctcttgaaga tatgcaaata aaaactgtgc caaatggaat
     8281 gggatataaa gaaaatccta atccagaaca ttataatata aatgtaaagt ggtatgagag
     8341 ttggaaaatt gaaatagagc aaactgctag atatgttaaa ggtataaaaa ataaatatcc
     8401 agataaaagt ataggtatat tggttccatt taatgaacaa gtgacacaag ttgctaaaga
     8461 acttagagag aataatctaa cttttgaaga attaggacca aattctttga ataaaaggaa
     8521 ggttataaac aacatagcat atattattga ttttatgttg cattgtgatg atgtagaaaa
     8581 gttaatagtt gtacttaata aagtttttat agatactgat aatgaagaag aaataaagta
     8641 ttttttagaa gaattaaaaa actattctac cgaagaaatt atttataatt ttgaatttag
     8701 taagttagat aatgatttaa tagaatttaa agaagaaagt gatatatact taagttttaa
     8761 aagaggaata gatattataa aacagatatt agaatatcca ataataaggt tagacctctt
     8821 agttttgttt attgggaaaa aacttaattt agaaaaagaa gataaagctg tactggatta
     8881 tatatctttt tatataaagt atctagtatc agaaaatata aatatggatt tagaagatgt
     8941 atataattta ttgtttgatg taaagaacag ggtgtttaat catataatag atgtggtata
     9001 tgagataaat ggatatgaac cagagcccgg tagtataaca ctatgtaact atcataaatc
     9061 aaaggggatg gaatgggatt gtgtgttttt actaggtctt gttgaatata atttccctga
     9121 caatattaat caaaagtttc agagtgataa atggtatcta aaagaaaaat ataaaaaccc
     9181 tatggcaatt attaaatcag aagttgaagc tatattaaaa ggaagtattt caacagatta
     9241 tgctcataaa acgaagattg attctataaa tgaaaagatt agacttttat atgtagggat
     9301 tactagagca aaggaaatgt tagtactttc aggaagtgct tatagagatg aaagtgatat
     9361 agggaataag agaaaagagc aacaaccatg tatatattta agcagactta atcaacatat
     9421 tattgaaaaa aggtcaaatt aatatataat aagttattaa aagattgctt gaaatatatc
     9481 taaaagggga taaaacaata tgtacagtaa gtaactacta aaaaataaag ttaagagaga
     9541 agtgatatga gtgaacgata aattaaaata tttttcttat agtcaaaatt caattaacac
     9601 atataaatca tgtccattga aatttaaata taaatatatt gatagaataa attggaaaaa
     9661 tgatgatgtt ggtagtagag agtattatga aactttaaaa acaggaagag attttcattt
     9721 gatttgtgaa agatatttta gcaatatacc tcttggaatc tattttaatg aggatgataa
     9781 aaatagtaaa aagtttttaa agtggatgga aaatataaaa aaaattgttc ctattgaaaa
     9841 aggaaaaaca tatttgccag aatatgaagt aaggatgaca ttaaatgggg atataataca
     9901 ggcaaagtat gatttagttg taattggaaa taacaatata gaaatatggg attggaaaac
     9961 cgagtctaaa aagattgact ataaacacgt agaaaataga atacaaacta ttatttatat
    10021 gtttttagca aaagaagtaa tacctaaagt tttaaaaatg gacataaatg ttaaagatat
    10081 aaatatgaga tattatcaac ctgaatttga tgacttacca attaccattt tatatgatga
    10141 agaaaaacat gaagcaaata gaaataaaat tcaaaattat ataagtatga taaaaaacac
    10201 taattatgaa gaacatacat acgaagacaa tctatacaat gatatagaaa aggtatataa
    10261 taaagaagaa cgtatgtgct atagaaataa aaagcattgc aagtattgtg aatttaataa
    10321 gttatgtaat ggcaaagaaa tagattatag tattttggag gcggaaattt atgggactta
    10381 ggtttgtatt aggtagaagt ggaagtggaa aatccaccta tatactagat gagattaaaa
    10441 aagaagctca aaaaaatgaa acgacatcaa ttatactttt agttccagaa cagtacacat
    10501 ttgaggctga aaatagagta agtaagctat tcttaggaaa agaaaaagat aagtatctaa
    10561 gagttagggt acttagcttt aagacactaa gtaatatagt tttttctcaa gtaggaggat
    10621 tgacagatgt aaatataaat tctagtggaa aagcaatgat ggtatataga gctattgaag
    10681 atgtaagtga agaattaaat gtgttttcaa aatcaaaatc gcagtctggt tttgtaagtt
    10741 caattacaga tatgatatca gaaatgaaac aatacaatat aagccctgaa atgttagaaa
    10801 atatatctgg agaattggat aatgaaactt taagtttaaa attaaaagat ataagtaaaa
    10861 tatataattc ttttgaaggg aaattgcatg aaaattatgt agatgctcaa gatatgttaa
    10921 cttcattggc aagtaaaatt gaattaagca gttatttaga tggagcttgt gtttatatag
    10981 atgaatttac aggttttaca ccaaatcagt acaatgtcat aaagagtata ttaaataagt
    11041 ctaaaagtgt taatatctct ttgactgtag atgatatcaa ctatattggg tacagtaaat
    11101 ctgatatgtt ttctagaacg aaatttactt attcaaaatt gactcaattg tgcaacgaag
    11161 aaggcataaa aatattgcca caagtaaatt taaacacagg tgttataaaa agatttgaaa
    11221 aagttaagga attacagcac ttagaaagat tttataatgc ttatccatat aaaatatatt
    11281 caaatccaac tgaaaatata aagataaaag agtttaacaa tttatattca gaggtggaag
    11341 agatagccag agaaattgta catttagtta gagataagaa tgtcagatat agagatataa
    11401 ccattgctac aagagattta aatagatatg attttttagt acactctata tttaatgaat
    11461 ataatatacc aaactttata gataagaaga gagaggcaaa aagtaatcca atagttattt
    11521 taattatttc agctttagaa atgaagaata gacgatatgg gtatgaaact atgtttaggt
    11581 atttaaaaag tggacttata ggaattgata atgatgatat aaatttacta gaaaattatg
    11641 tgttggctaa tgggattaaa ggtaaaaaat ggtttgatga aaaatgggat tatagaataa
    11701 ctcaaagtct ttcaggtcaa gaaagtgaat ttgaactgga attaaaagag aaaataaatg
    11761 aaataaaaaa tagagtatta gagcctatag taattttaca agaaaaatta agaggaaaaa
    11821 atagagtaaa agaaatctgt agatatatat atgaattttt attagatata aatatgccag
    11881 aaactataga aagtttaata gtaaatttta aggataaagg ggaacttgat gttgcaaatc
    11941 aatactcaca agtttgggac atagtagtgg acatattaga ccaaatggtg gaacttatgg
    12001 gtgatgaaat aatttcttta gaaaaattta taaaattaat aaccttaggt tttgatgaat
    12061 atgaattagg actggttcct cctagtatag accaggtact tgtaagtagt gtggatagaa
    12121 tgaaaaatcc agacacgaaa tatttatacc tagttggaac tactgatggt gtgtttccgc
    12181 tgattacaaa ggatagtgga atattgagtg ataatgatag ggaaagtctt ggaaacaaag
    12241 gaatcgaagt tgatattgat agtaagacta ggagttttga agaacagttt cttgtatata
    12301 aagctcttac ttcgacatct aagaatttaa ccataacata tccaatatca gaccatgaag
    12361 gtaaaacgct tagaccatca ataattatat caagacttaa gaagatattt ccaaatatag
    12421 aaaataaaag ttatttagtg gaagaaaata aaagcactga taaagatata cttaaaaaaa
    12481 taacggttaa atcccctaca tttaatgaac ttataaacat aataaaaaat tatgacagtg
    12541 atggttacaa tacagaagag attaatagta tttggttaga tatatatagg tactatttaa
    12601 aagatgaagt ctatagttct ataacaaaaa aagttataaa aggtcttagt tatactaatc
    12661 aagttcataa gattgaagag aaaaaaataa ggtcattgta taaaagtaat tctcttagca
    12721 tatctaggct tgaaaaatat gctgaatgtc catttgctta ttttatacaa tatggtctaa
    12781 aagcaaaaaa gcgtaaagag tattcattta caccaccaga tttaggaact tttattcata
    12841 atattttaga taggttttca aaagaacttt tacaagataa tttaacttgg agagatattg
    12901 atgaaaaata tatagaatta aagataggta taatagttga tgaaataatt ttaaagatac
    12961 caggatatat attaaatagt tctgagaggt ataaatacct tgcatataga ttaaagaata
    13021 tgttgacaac tgctataact ataattagtc agcaaataaa acaaggttca tttgaaccaa
    13081 tagattatga agttaaattt ggcgacaatg gaaaatatcc acctataaaa atggtgttag
    13141 aaaatggaca agaagtaagt ttaattggac aaatagatag agttgatgaa tttgaagaag
    13201 gagaaaataa atatataaga ataatagatt ataagtcagg caataagtcc ataagtctaa
    13261 cagaaatata ttatggtcta caattacaat tacttgtgta tttagatgca atccttgaaa
    13321 gtacaaagga tgaagatata aatataaatc cagctgcaat actatattgt agaataaata
    13381 atccaatagc aaaatttaat gaagataaag atgatgaaga aatacaagaa gcaatattaa
    13441 aagaacttag aatgaagggg cttgtagtaa aagactctca catagttaaa gagatggata
    13501 aatcattaat agatggagaa aggaaaaact ctttagtaat accagtaggt cttactaagg
    13561 atggaaatgt aggtaaaagt acttcagcaa taagctatga ggatttcaaa ctgcttagaa
    13621 aatatgtaag gcatgctata aaggatttat gtgaagaaat gttaagtgga gaaatacgta
    13681 tagctccata taaacataaa gatggaacat catgtgactt ttgtgattac tctgctatat
    13741 gtcagtttga ttcaactata aaagataaca agtataaaaa tttaaataat aagagtaatg
    13801 aagagataat aaaaatgatg aaaggagatg ttaattagtg agttctccta aatggacaaa
    13861 agagcaatta gaagttatag aaagtagaga atgcaattta ttggttgctg ctgctgctgg
    13921 ttcaggaaaa acagccgtgc tagttgagcg tataattcaa atgataacaa gtagagaaaa
    13981 tccaattgat atagataagt tattagttgt tacatttact aatgctgctg cgtcagaaat
    14041 gagagagagg ataggggatg ctataggaaa agccttagat gaaaacccag aaaataaaca
    14101 tttacaaaac caacttgttt tattaaataa atctagtatt acaacaatac actcattctg
    14161 tttggatgtt ataaaatcaa actttcatag aataaactta gaccctaatt tcagaatagg
    14221 agaccaaact gaatgtgcta ttttgaaaca agaagcaata gaggaagtat ttgaagattt
    14281 atatgaagaa agagatgagg ggtttttaaa tttagttgaa agttatgcag aaagaggagg
    14341 agataaagaa gtacaagata ttatcttggg aatatattca tttgcaatgg cttctcctga
    14401 acctaaaaaa tggttgattg actcagcaga acgatttaat attgatgaaa attttgattt
    14461 ttcacagtct atttgggcta gagctatatt agatactgta aaaatagaga ttaatggtct
    14521 ttgtctaaat atggaaaggg cattaaaaga agtggaatca atagaagaat tagaaacttt
    14581 tgcagaaaag ttatccgttg aatataaaaa aattgctgac atttcacaag catgtaataa
    14641 atcttgggat gaggcatata aaaagatggc aagtatgtcc tttgaaaatt atgtaaaagg
    14701 agtaaaaaga atttcaaaag atgctccttc gtatataaaa gaatcaaaag aaaaggcaaa
    14761 aaccataaga gataagacta agaagtcttt agaaagtata gtaagtgcta cattcaataa
    14821 agataatgat tctattagag aagaaataaa atatctttat aacatagtta aaccaatatc
    14881 tagtgttgta ctgagatttg aagaggaata tagcaataag aaaagagaaa aaggaataat
    14941 agattttaac gatatagagc attttgcatt aaatatactt acagatgtag atgaaaaagg
    15001 taatattgta ccttctgata tagcagttgg atatagaaac aagttttatg aaatatttat
    15061 agatgaatat caggatagta atctagtgca ggaagtactg cttaaagctg ttgcaaatac
    15121 agaaactcca aatagattta tggttggtga tgtaaaacag agtatatata gatttagaca
    15181 agcaaagcca gaattatttt tgcaaaaata taataactat aatgataaaa agggaagttc
    15241 ccatagaaaa ataatgcttt acaaaaattt tagaagtaga gaagaagttg tagatgctgt
    15301 aaattatata tttgaaaata taatgaatga gaatatagga gaaattgaat atacagaaaa
    15361 agaaagacta aatttaggtg caaattttaa tgtggataca gacgagaaat ctattattgg
    15421 tggagcaacg gagatacacc taatacaaaa agataataaa cttgatgatg atattataaa
    15481 tgataaagac gatagaatta ataacaaaga agatgaaata gaggaagaag aaaaattaga
    15541 taatatacaa cttgaggcta gaatggttgg aaacattatc aaagatttaa tgaaagtcaa
    15601 tgaagatgga aaaattcaaa aggtctatga taagggaata gatggatata gacctgtgga
    15661 atttagagat atagttattc ttttaagagc tacatctgca tgggctccag tgtttgcaga
    15721 tgaattgatg aatatggata taccaacgta tgctgatgtt ggagtggggt attttgatac
    15781 gattgaaata aaaacaatat tgtcattact tcaaataata gataatccaa tgcaagatat
    15841 acctcttata tctgtattaa aatcacctat atttggattt acaccagagg atttgattga
    15901 tattagagtt cagagtaaag ataaaatttt ttatgaggtt ttaaaaagta cagcagagta
    15961 tgatggattt acagattctc aaaatgaaaa tgagtctgag tttataccaa gtgaagagtg
    16021 cataaacaaa agtaaagatt ttttaattaa gctaaaggaa tttaaagaaa agtcaatgta
    16081 catgagtaca gatgaattta tatggtactt gtacacaagg actggatact atgcctatgt
    16141 tggagcattg ccaggtggaa gtcaaagaca agctaattta aaagtattgt ttgagagagc
    16201 aaaacaattt gaagaaacca gtcttaaagg aatattcaac tttgtaaatt ttatagagaa
    16261 attaaaaaaa tctagttctg atatgggaag tgcaaaaaca cttggtgaga atgcaaatgt
    16321 tgttagaata atgagtattc ataaaagtaa aggtctagaa tttccagtag tcatatgctc
    16381 tgcaatgggt aaaaatttca atactcaaga ttttaaaaag agtattttat atcatcataa
    16441 tttagggtat ggacctcaat ttgtagacta tgagagaagg atttcttttc caagtatagc
    16501 caaagaagca ttaaaaagca aaataaatat agaaaattta tcagaagaga tgagagtttt
    16561 atatgttgca tttacaagag ctaaagaaaa attgattata actggttcta ctagaaatat
    16621 acaagatagt ataaagagat ggtcaaatgg aattgaaagt ttagatacaa tatcacaata
    16681 cgagatatta aaaggtaaaa attttttaga ttggataatg ccctgtgtat taagacatag
    16741 ggatttatct aatctactag aagaagtagg attagacgca gtttttaatg ttgaacataa
    16801 ttcaaaatgg tatggaaagt tgtggaataa gaatgatatt ttagtggaaa agaagagtga
    16861 tgaagaaaaa gaaagtattg aagaaattct tgaaaaaata gatgtagata atcctgacag
    16921 tgattattac ggtgaaatag aagaaaaatt aaactatata tatccatatg aatttagtac
    16981 aagaaaacct gctactatat ctgttacaga aataaaaaag atacaaaata attatgaaga
    17041 agagttaata aatactatat ttgaacaaaa agtaatatta aaaaagcctc tatttattca
    17101 aaatgaagaa gaaagagaaa aaataagtgg aacagaaaga ggtacagtag tccatttagt
    17161 tatggaggtt ttagacttaa aaaatgtaag tagtgtaaat gatataaaat cacaaataag
    17221 aggttttgtt tcaaaaggta taataacaga aaaacaagct agtatagtaa atccatataa
    17281 aatatataaa ttttttgcct ctaacatagg aaaaagaatg ttgaatgctg aaattataaa
    17341 tagagaaaaa tcaatttatg cacaagtaaa tatgaaagac atatatattt atgaaaagct
    17401 tataaacaat gacgataaaa aactttatga taatgaaagt gtcatgttaa gaggtatagt
    17461 tgatgcttat tttgaagaag ataatcaaat agttttggtg gattataaaa cagattttgt
    17521 aaatgaggaa aatataaatc aaataataga gaaatataaa aaacagttgg atttatatgc
    17581 agatattatt gaaactttaa ctggtaagtc agtaaaagag aaatgtatat atttatttgg
    17641 agttgatgaa gctgtttgtt attagttttt atatataaat taaacctaaa agatagagga
    17701 aggggaaaaa atgagattta ttcatacatc agattggcat ctagggaagt ccctagaagg
    17761 ccattctaga attgaggaac aagcaaagtt ttgtgaggaa tttataaaaa tagtagaatc
    17821 aaatgaaata gatatggtta taattgctgg agatgtatat gatacttcta atccaccagc
    17881 acaagcagaa aaactatttt atcagactgt atcaagactt gccaataatg gtcagaggtg
    17941 tgtattaata atttcaggaa atcatgataa tccagagaga ctctctgcaa taacacctct
    18001 agcacatgaa caggggatac ttatatatgg ttatccatta agtgctacga tagaagctaa
    18061 gtataaaggg tttgaaataa catatgcaac tcagggatgt acaaaactga atataaatgg
    18121 agaaaatatt gtggttgcta cattacctta tccaagtgaa aaaagattaa atgaagtttt
    18181 ttcaagtgaa gatgagtttg agaaacaaaa aaattattct gaaaaggtag gagatatatt
    18241 tagaagttta gaagaaaatt ttagaagtga tactataaat atagccgtat cacatatatt
    18301 tgtaataggt ggagagagta ctgaatcaga aagacctata cagttaggtg gaagtttttt
    18361 agttgaaaga aaagatttgc cagaaaaggc tcaatatact gcactaggtc atttacataa
    18421 acaacaaaaa gcttccgaac gtttaaatgc atactattct ggttctccac ttcaatatag
    18481 caaggatgaa agagcgtata ccaaaggggc atacatagtg gatataaaag ctggtgaaaa
    18541 accaataatt gaagatgtat attttaataa ttacaaacct atagaagtat ttaaatgtaa
    18601 tggaatagaa gaagcactag atatatgtga gcaaaatcaa gatagagaga tttggtcata
    18661 ttttgaaata aatactgatg agataatatc acagaatgaa attaaaaaaa tgaaggaact
    18721 attaaaagac ataatagaaa taaaaccaat aataacatct tgttatgaac aagaaagtgt
    18781 agatataaaa gaaaagtcta tggcagagtt gtttagagag ttctattctt ttagtaaagg
    18841 tgtagagcca aaaggagaac ttatggattt atttctagat ataataagtg aagaaggtga
    18901 atcagcagat gagaccaatt agattagaac ttacagggct taatagctat attgataaac
    18961 aagttataga ctttgaaaaa ttaattgaaa gaggtttgtt tgggatattt ggaactactg
    19021 gaagtggaaa atctacaatc ttagatgcaa taactattgc tatgtatgga aatatatcca
    19081 gaaatacaaa agaatatata aacagtgtgt gcgataaggc aattatatca tacgagtttg
    19141 agataggaag caaaaacact agaagaagat acattgtaga tagaaccatt gctagaagca
    19201 aaacaggaac aaagacctct aatgctagat taatagaagt attgaatgac aatacccaaa
    19261 atgttttagc tgataaagtt gtggaagtaa atgaaaaagt agcacaggtt gtaggattaa
    19321 cagcaaatga ttttactagg tcagtagttt taccacaaga caaatttaat gagtttctaa
    19381 gattatcacg tgcagataga agagacatgt tagagagaat ttttaatctt gagaaatatg
    19441 gaagaagtct tggagaaaaa gtgaaaaaga gaaaaaatat acagttacaa aatttaaaag
    19501 atttgaaatc aaaattaagt caatatgatg gaattacaga ggaagtatat aacaatataa
    19561 accaagaatt aatagagtta aaaaatttgg aaaaagataa gaataatgct ttagacctag
    19621 ctcaaaaatc ttatgaagaa agtaaaacag tatatgaaga acaattaaaa ttagaaaaaa
    19681 atgaacttag aaaaaatgag cttgatttaa aaaatagtga gataaaagaa aagattgctc
    19741 ttgtagaaaa tgatagtaac gctaaaaagg tagacccata cataagttca gttcaaaatc
    19801 tagaaaaaaa gatagatgaa gatagctttg cagtaagtaa tttagaaaag aaattagcaa
    19861 tattaaatca ggagcttgaa gttacaaaaa atagatatga aaaaataaac aaaattaaga
    19921 atgaagaagt ccctaaattg agtgaagaaa aaataagatt gcaacaagca ataaaattag
    19981 aagaagagtt agttttatta gatagagagt taaaagagct aaaagaaagt ggaattaatt
    20041 taaataaaac taaagttgaa ttagaaaaag taaagcaggt gtcagagtcc agaaaagatg
    20101 cagttactaa atctataaaa gaagttgaag gaaagattga taaagtaaat ataagtgcag
    20161 aattaaaaca aaaaatattt ctagcatatg aatacgagaa agattacaac aacgtattgg
    20221 aagaaaaaaa tcaaaagcta aataaactag aagaaatact caaagagaca gaaaatataa
    20281 atttaaaagt tagatatatt gataaagata agaatgatgt aaatcgtaat ttagaaaatt
    20341 tatcacttca cttggatgtt cttcttaaaa aatgtcctgg taagagtgct gatttacttt
    20401 taaaatctga atatgttact gaattaagaa ataaagcaaa taatactaaa gaaaatgaaa
    20461 ttaagaaaag cagtatacaa gatgaattaa agataatact tgaaagtaag tttaatactg
    20521 aaagggaagt aaatttacta aatgaaaaac ttgaaaataa tagaaagaac agagatgacc
    20581 tagaaaaaga attagaagaa ctaaagtatt taaatttagc ctctgaactt agacgtgaat
    20641 taaaagaaaa tatgccttgt ccagtttgtg ggtcaaaaca tcatgaaaat catgatataa
    20701 ctaagtatga tgaaaatata tcttttgtca aagaaaaact ggagaaatta gaaaaagaaa
    20761 aaataagtat aagaaacaat atagaggaat taaatgctaa agtaagtggg tatttatcta
    20821 tagaaaaaat gaagactaaa gaacttgaag atgtaaaagg aaaactaggg gggattccat
    20881 ctagtcaatt attaaaaaaa cttgatgaag aacaaagaaa acttgcattg ttaaaatcta
    20941 atattcagga gtgggagaaa gaaaaagaga gtactgaaaa taaagtaaca ttagctaaag
    21001 aagaaaaaaa taaaattgaa aaagaagaat taaaaataag agagagtctg aataattata
    21061 agaaattaac aagagattta aacatagaaa tagaaggttt agaaaataaa tgtaaaaaaa
    21121 ttaaacagga atacctagga ttaaagacga ttactaaagt ctctaatcta ttgtcaaaag
    21181 taaaagaaat aagagagaat gaaaaagaat tagaattact aagtactggg cattcaaatt
    21241 tattaaaaaa tagagactca ctagatatta aaataagaga aaatgagagt aaactacatg
    21301 aaatagagat agaattaata aaggctagag aattatatgt agaaaagaaa ttgagtagag
    21361 ataataagta taaagaagtt attagcataa cgaaaggtga tttagctaaa aatcttttac
    21421 ataatgttga agaaaatata tgtaagatat tagaacaaga agaatcaagt aagaaaaaat
    21481 tggaagagca aagattagaa tatgaaaaaa acattgctga aaagcacaat atagatggta
    21541 gacttaaaac tgctaaggaa caatataaag accaaaaatc aacactaaat aaactattgg
    21601 cagaaaataa atttgaaagt atatatgcag taaaaagagc tttattagat actgatgcag
    21661 taaaaaattt gattgaagaa atactagagc atgaagaaga acaaaagtta ttatcattta
    21721 aaataaaatc atcaaaagaa aaattaaatg gaagaagcat aaagaaggag tactttgaac
    21781 agttaaaaga tgaaatttat aatttgaaag tagaaatagg aaatatatcc aaagatattg
    21841 gagctaatca aaatcagtta ataacattaa aagactcttt agataagata aatgatttta
    21901 ataaacaact taaggtagtt gaacacaatg tggacttatt ggaagaatta gataaatgtg
    21961 tacaaggaaa taaatttgta gaatatgttt ctacaaacca attaaaatat atagcgttgg
    22021 aagcatcaaa acgattggat ggaataacaa aaggaagata tgcacttgaa attgactcaa
    22081 cactaaactt tgtaatgaga gataacttta atggaggaga aagaagaagc attgatactc
    22141 tatctggtgg agagacattc ttgacatctc tatcattagc acttgctctt tcatctcaaa
    22201 tacaattaaa gggaagtgca ccattagaat ttttcttttt agatgaaggt tttggttcat
    22261 tagatagtga acttttagaa attgttatag aatcattgga aagattgcat agtaataatc
    22321 taagtgtggg tataataagt catgtcgagg aacttaaaaa tagagttcct gttaagctat
    22381 tagtttcatc tagtgaggct ggaataggtt caaaagtaaa aattgaatat agttagaaaa
    22441 tatatgttaa ttgaatatat tatctaatat ttccaaataa tatgtaattg tgatataata
    22501 aaaactaata ttaaagttat ttataattaa tattaatata aaaacatgta agtaggagtg
    22561 tggtaagtgt tggaatcgcc caataatttg attcagatta tttttttaat agtattactt
    22621 atagggtctg catttttctc agcatctgaa acggccttaa tgtcgttgag taaaatcaga
    22681 atcagatata tgcaagatga aggagttaaa ggagctaagt tagtaagttc attaatagaa
    22741 aatccaaata agcttttaag ttctatattg gttggaaata acgtcgtaaa tatagcagca
    22801 acatccatat caacatcttt gtttatagga ttaatgggag aaaaaggagt tgcacttgct
    22861 acagctgtta tgacagtatt agtattaata tttggtgaga ttactccaaa aacaatagca
    22921 gcaaataatt cagaaaaggt ttctctatta gtatctaaac ctataaaagc aattatattt
    22981 atattaagac caatagtatg gatatttaat ataattacaa atattatatt taaattattt
    23041 ggtataacaa ataaaggagc taaatcattc ataactgaag aagagctaaa aactatggta
    23101 aatgtaagtc atgaagaagg cgtccttgaa atggaagaga gagaaatcat aaataatgtt
    23161 tttgaatttg gagatatgca agctaaaaat gcaatggtac aaagaataga tatggtagct
    23221 atagatatgg aagatagtta tgatgagata atacaggtat ttaaaactga aaaattaagt
    23281 agaatgcctg tgtatgaaga aactatagat gatatcgtag gaatacttaa tataaaagat
    23341 attatatttt tatctgatga agaaatagag tcatttgata ttaaaaatta tatgagagaa
    23401 ccttttttca cgtatgagtt caaaaagata acacaacttc ttgaagagat gaaacttgaa
    23461 aaaagtcaga tggctatagt tgtagatgag tatggtggaa catctggact tttaactata
    23521 gaagatttag tagaagtcat tgttggagat atagaggatg aatatgatga agaagaggat
    23581 gaaatacaag ttataaaaga agatgagtat attgtagatg gaagtactaa aattggtgat
    23641 gttaatgaac ttattggtgt aaacttggag tctgaggagt ttgattccat aggag
//
LOCUS       DAGSHT010000037        23570 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGSHT010000037 DAGSHT010000000
VERSION     DAGSHT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.038"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            75..1151
                     /locus_tag="MJB72_001359"
     CDS             75..1151
                     /locus_tag="MJB72_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY8262480.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1254..2096)
                     /locus_tag="MJB72_001360"
     CDS             complement(1254..2096)
                     /locus_tag="MJB72_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY8262481.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2280..2852
                     /locus_tag="MJB72_001361"
     CDS             2280..2852
                     /locus_tag="MJB72_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8262482.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3015..3287)
                     /locus_tag="MJB72_001362"
     CDS             complement(3015..3287)
                     /locus_tag="MJB72_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262483.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3414..4286)
                     /locus_tag="MJB72_001363"
     CDS             complement(3414..4286)
                     /locus_tag="MJB72_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY8262484.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4582..7689)
                     /locus_tag="MJB72_001364"
     CDS             complement(4582..7689)
                     /locus_tag="MJB72_001364"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBY8262485.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7782..8014)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8189..8716)
                     /locus_tag="MJB72_001365"
     CDS             complement(8189..8716)
                     /locus_tag="MJB72_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBY8262486.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8751..9536)
                     /locus_tag="MJB72_001366"
     CDS             complement(8751..9536)
                     /locus_tag="MJB72_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBY8262487.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9546..9803)
                     /locus_tag="MJB72_001367"
     CDS             complement(9546..9803)
                     /locus_tag="MJB72_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBY8262488.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9822..10280)
                     /locus_tag="MJB72_001368"
     CDS             complement(9822..10280)
                     /locus_tag="MJB72_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBY8262489.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10320..11024)
                     /locus_tag="MJB72_001369"
     CDS             complement(10320..11024)
                     /locus_tag="MJB72_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBY8262490.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11034..11669)
                     /locus_tag="MJB72_001370"
     CDS             complement(11034..11669)
                     /locus_tag="MJB72_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262491.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVNTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12050..13090
                     /locus_tag="MJB72_001371"
     CDS             12050..13090
                     /locus_tag="MJB72_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8262492.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13553..14908)
                     /locus_tag="MJB72_001372"
     CDS             complement(13553..14908)
                     /locus_tag="MJB72_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBY8262493.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(14934..15203)
                     /locus_tag="MJB72_001373"
     CDS             complement(14934..15203)
                     /locus_tag="MJB72_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY8262494.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15493..17448)
                     /locus_tag="MJB72_001374"
     CDS             complement(15493..17448)
                     /locus_tag="MJB72_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262495.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKNIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIDKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17631..19106)
                     /gene="spoIVA"
                     /locus_tag="MJB72_001375"
     CDS             complement(17631..19106)
                     /gene="spoIVA"
                     /locus_tag="MJB72_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBY8262496.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19278..20573)
                     /locus_tag="MJB72_001376"
     CDS             complement(19278..20573)
                     /locus_tag="MJB72_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8262497.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(20757..22166)
                     /locus_tag="MJB72_001377"
     CDS             complement(20757..22166)
                     /locus_tag="MJB72_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY8262498.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(22218..23357)
                     /locus_tag="MJB72_001378"
     CDS             complement(22218..23357)
                     /locus_tag="MJB72_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY8262499.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
BASE COUNT         7478 a         4179 c         2669 g         9244 t
ORIGIN      
        1 agaaaagtat actctttttt gtttaataat gctaaaattg atttgtattg aattttgatt
       61 aggaggaaat tattatgaaa gctcaaagat taaatgctgt acttgaacaa atgaaaaaag
      121 atgatatttc tcaaatgtta gtaagtgacc ctacttctat attttatctt acaggagtac
      181 taatccatcc aggcgaaaga ctattagcat tatacttaaa tttaaatggc aataataaat
      241 tatttataaa tgaattattc ccagtttcag aagatttagg tgttgaaatg gtttggttta
      301 atgatactca aaatccagta gaaataataa ctgaacatat agacaaaaat gctactatgg
      361 gtgttgataa aaactggcca gctagattct tattaaactt aatagaatta ggtggaggaa
      421 gtaagtttgt aaattcatct tatataatag atactttaag aatgtgtaaa gatgaagaag
      481 aaaaagagtt aatgagaata gcttcaaaat taaatgacaa ggctatggaa caacttaaag
      541 ctactgtttc tggtgaactt acagaaaaac aacttgttgg taaattatct aaaatatatg
      601 aagacctagg tactgatgga ttttcatttg accctattat aggttttgga cctaatggag
      661 ctaatccaca tggtgaacct ggaaatgcac ttgtgaaacc aggagatgca ataatcttag
      721 atataggttg tataaaagat aactattgtg ctgatatgac aagaactgta ttctataaag
      781 aaatacctga aaaaggtaga gaaatatttg aaatagttct tgaagctaat aagagagctg
      841 aagctattgt aaaaccaggt gttagattct gtgatataga tgctgctgct cgtgactaca
      901 taactgaaaa gggatatgga cagtacttca ctcatagaac tggtcactct ataggacttg
      961 aagttcatga taaaggagat gtatcttcta taaatacaga tactgtacaa ccaggaatga
     1021 tattctctat agagccaggt atatatttac caggtgagtt cggagttaga atagaagatt
     1081 tagtacttgt tactgaagat ggttgtgaga tattaaacaa acatgacaaa gaaatatgtg
     1141 tagttggtta attctaaaat caatttgata aaattaaaaa gccatataaa aatgttttaa
     1201 acatttttat atggctttaa tttctcaatc tatgatttat ttcaatccat tatttatatt
     1261 gcagttatta agattgcacc ctttgaagta tatgtcccca tcactgaacc aatttcacag
     1321 attacaacat ctttgtattt tacatccttt tccataactt cccttacctt ttgagccttt
     1381 tctgggcaat tagcatgtcc tataaataaa accttttctt ctttatcttt aatttttgac
     1441 tctactaaag taatcagctt tttcaaactg tttccttcac ctcttgcctt atctatagga
     1501 gttacaacgc catctgcaac ttgtattatc gccttaaaat ttaaggtatt tataattttt
     1561 cctgctattg gatttattct accaccctta atagcatttt ctaaagtttc aagtgttcca
     1621 aaaaatggag gattatcttt atatttattt attattgaaa caatctcttc aatttctttt
     1681 ccttctttta ttaatattgc tgcttttaac actaattgtc ctattccaat tgacccacta
     1741 agtgaatcta taacctcaac tttcttgtta tttccttcct tttcaaacat atcttttgct
     1801 aaaagtgcac tattataagt cccagataat cctgaactta tattaatcac taatacactt
     1861 tcttcctgac aatggtatgc ttctaaaaat ttatcaggag aaggacatga tgtttttggt
     1921 agtactttac tctcactcat ttttctatag aaagtttcat tatctatttc ctttccagat
     1981 atataacttt cttctccaaa agatacattt aaaccaacaa cttctatgtt gtatttttct
     2041 attatatcat caggtaagtc acatgcactg tccactatca attttatttt atccatatta
     2101 acctcactat aagatattat tttatttatt cagtcggtcg tacgaccgat ttaattatta
     2161 ttttatatat tgaatgaaaa aaagtcaata gatatagatt ctcgctttat tgacaataaa
     2221 tatccataag cttataatgt tattaatttg ttataaatat acatggaaag gtaggtttta
     2281 tgaaaacaaa taatatgaaa caggctattt tatcatctgc aaccaattta atagccaaaa
     2341 atggagttca aaatactagc cttgctgata ttgcaaaaga tgtaaatata agtaaaggta
     2401 ctttgtatta tcactacgca tctaaagatg atattatata tgatattgcg gatactcatt
     2461 tagaagttat aactagtgct atactaaact gtgtaaaaaa tgtcaaatca aaaaattctc
     2521 aaatagaaat ggttaactta atcttagaga aaatatctac tatagaaagt cgtggtaggg
     2581 tacatatgta tcttatttgt gaggcaatta cttcaaataa tgatttaaag gaaagaataa
     2641 gattaaaata tattgaatgg agaactactc ttaaatctga aattgttgaa tcactagaaa
     2701 aatataatag tagcgaaaat gaacaggatg ctgaatcatt ttctttttta ctcatgtcaa
     2761 tagttgatgg tttagttgtc caaagtttat taaaaacaga aaaaatacct tatgaaaata
     2821 tagcttcttt tttagttaat cattggattt aaacttaata cttagataat taataaaagt
     2881 gaactattta agttatctac tttaacagtt cactttctta tttattctca ataattttta
     2941 cttaaaacat tcaattttta tttaaacctt catggaatca caaaataaaa atctcttata
     3001 aatttaaaat gtttttattt atgttcatgt gtatgcccat ctgaatgttc atgaccatgt
     3061 gtatgtgtat gctcatggct atgctcatga gtacctgaaa aaccatcatc atgattatgg
     3121 tcgtgaccta caccatttac atgctcatgt tcatgcccat gactgtgaga atgttgatgt
     3181 gaatgttcat ggttatctcc tccacagcat ccttctcctc cagtcccaca acacccactt
     3241 gtatgtggat tatgatgatt tcttactaca aatctctctc ttaacataat tcttatacct
     3301 cctagtatat tattgagtac ttcatgtacc ccattaacta cagtatacat ctaactaggt
     3361 ataatctcaa tacatatggc attttttaac atatataatt aacatatata atcttaaaga
     3421 aatttatcct taaaacctcc accttttact tcttcaacgt tattgacaga aataaaagct
     3481 tcactatcca tatcataaat taaatcttta aatttaccta tttcatttgc agatattatg
     3541 caataaatca tctttttctt attttgtgta tatgcacctt ctgcctccaa aaaagtgact
     3601 cctctaccca ttttattcat tatcactttg gatatttcat catatttatt tgatattaaa
     3661 agtatagatt tttttctatc aaagcaatct tttcctatat ccatagtaaa agaaattata
     3721 tacattgtaa ttaaagtgta catcaccaat ttagctccaa ataaaaaagc tcctgcacat
     3781 actacaaaaa aattaataaa taaaaatgta tttttcatag gtatatcata ctttcttttc
     3841 attatggctg ctattatatc aagtcctcca gcagtagctc ttgctttaaa agttaacccc
     3901 atacctatac cagttaatgc accaccataa atacactgta gcatagtatc atctattggg
     3961 aaatatttag caatatcttg tgttagcccc agtgctagag aaaatagtaa catattgaca
     4021 aaacttatta gcaaaaattt attatcaaaa tactttctag ctataataaa tattggtatg
     4081 ttcatcaaga aacttgatat tccctgatta ataccaaata atttgtataa tataacacaa
     4141 attccagcta ctcctccact taataacttg gctggtgcta gatataaatt tattcctaag
     4201 gttgatagaa tcatacccaa tataataaga caatattgta aaaatatatt attatcaata
     4261 cttagctttt tactttcacc atacatcaaa acgcacccct ttgcataata aataaaccac
     4321 cctgcataac acacgataaa tttacaaata aatttatcag aagtagtttc ctaaaaatat
     4381 tattcatcct cacttaaacg tttattcatt aatattctat cacatgacat aaataattta
     4441 tatgatttat atagatttat ttattgtaaa ttttggaata ttaaaacatt ttattgaata
     4501 gataattata ttacataaaa aatccctata gaaatctata aactaatttc tatagggatt
     4561 ttggttaact aactattatt tttatttttt tataactttt atacctgtca tatggtcatt
     4621 taagttttgt tcctctaaag atttatcatc tactctgttt atttctaaag ctaaagtttc
     4681 agacttgata tattcattaa aatggtcaac agccttagca atttcatcat caccacagta
     4741 ttctattact atattatcta atacttcaaa gttattagat tttcttaatt gctgtacttt
     4801 agatataaat tctctagcat aaccttcatt aattaactct tcagttagct tagtatctag
     4861 tataacaaaa agattgtttt caacagatac attaaatcct tccttagcag ttatacctat
     4921 taatactaaa tccttattaa attcaaaagc ttcaccatct aaatcaactg ttaaagtctc
     4981 acctttttca agtttattag cagtttcatg agcatttaat ttacttaatg ctccaacgaa
     5041 taaattcatc ttagaaccta ataatggccc tgcttcctta aagtttggtt ttaaactaaa
     5101 gttcatatac tcatctaaat ccttagcaaa tattacctct ttaacattaa gctcttcttt
     5161 tattaaatca actacatcac ttattgtatc ttcaaactta ccatctacta acactttttg
     5221 taatggttga cgaactttta ttctttctac ttctcttgaa gctctaccta aagtaactaa
     5281 gtttttagct aaatccattt tttcttctaa tttagtatct actaactctt cattacattt
     5341 aggatattca gctaaatgaa cagatacttc tcctgttaag tttctataca tttcttctga
     5401 catatatggt gcaaatggag ctatagctct acaaagacca tgtaacaatt cataagttgt
     5461 attgtacact gcttttttat cttctgttaa ttcagttgcc cagaatcttc ttcttgaacg
     5521 tcttatatac cagttagata aatcatcatt tataaaatct tgtatcattc ttaaagtttt
     5581 atttaactca aatatagcta aattttcttc tacatctttc attaaattat tgaacttaga
     5641 taatatccat ctatcaagtt ctggtctatc tttatattct acaaaaaatt cagttggatt
     5701 tatatcatct gtgtttgcat ataaagtaaa gaagttatat acattcttca tagtacctat
     5761 aaatttactt tggatttctt ttaatccatc catatcaaat ttagttggag tccatggtgg
     5821 agaaacatat agtaaatacc atcttaatgc atctgctcca tattggtcaa ataattccat
     5881 tggatttact gtatttcctc ttgatttact cattttctta ccatctttat caagtacaag
     5941 atctggaact aaaacactct tatatggtgc ttttcctaat acaaatgttg atactgcaag
     6001 taatgagtag aaccaacctc tagtctggtc tataccttct gatatatagt cagctgggaa
     6061 caattggtca aagttttcct tattttcaaa tggatagtgg tgttgagcaa atggcatagc
     6121 cccactatca aaccaacagt ctataacctc tgttactctt gtcattggtt ttccacattt
     6181 ttcacatttt aagtgtatat catctacata tggtctatga agttcaactg tttctggatt
     6241 tacatcttct atagcttttt cagctaattc agctcttgaa ccaactgaat ctgtatgacc
     6301 acattcacat ctccatatag gaagtggagt tccccaatat ctagttcttg atattgccca
     6361 gtcatttaga ttttctaacc agtttccaaa tctcttctct cctacaaaac ttgggaacca
     6421 ttttacacca ttattatttt ctattaattt atctttaagt ttagtcatct ctatatacca
     6481 gcttggtttt gcatagtata ctaatggagt ttgacatctc cagcagtgtg gataattatg
     6541 aactactttt tctttgctat ataatttatt ttcagaagct agccatttta ttatttcaac
     6601 atctagccca tcttccatta caaatctacc ttcccaagga gtagatgtaa atttaccagt
     6661 ttcatctact ggttgaataa ctggtaaatc atatctttta cctgtgttgt agtcatcttc
     6721 accaaatgct ggtgctgtat gaactatacc agttccatct tcagtagtta cataatctcc
     6781 tactgtaata aaaaatgctt ttttgtcaac ttctacaaaa ggcattaatt gttcatattc
     6841 taaaccttct aggtctttac ctttcatttc ttctaaaact tcataatctt cacctagtac
     6901 tttatcagct aaaggtttag atacataata tacctcatca ccttttttaa cttttaaata
     6961 atctacatca gcatttactg ttaatgaaac gtttgatggt aaagtccatg gagttgttgt
     7021 ccatgctaag aagtattcat cagcatcttt tttcttaaat ttagctataa ctgtattagt
     7081 ttttacttct ttatatcctt gtgcaacctc atgactagca agaccagttc cacatcttgg
     7141 gcaataagga agtattttat gtccttcata tatatatcct tctttgttga atttgtttag
     7201 tatccaccat acagactcta tatagttatt atctaaagtt atatatggat tatctaaatc
     7261 aacttcataa gccattctct cagtcattat tctccattgt ttttcaaatg agaaaactga
     7321 ctctctacat ttttggttaa attcagctat tccatattct tctatttgtt gcttgctagt
     7381 aagacctaac tctttctcta cttgtatttc aactggtaat ccatgtgtat cccaaccagc
     7441 ttttctcttt acttgataac cagacattgt tttatatcta caaactgaat ctttaagcgt
     7501 tctagataaa acatggtgta ctccaggatt tccattagct gttggaggtc cttcatagaa
     7561 tacgaaactt gggtcatctt tccctttttc taaagttttt tcaagtatat ttatgtcttt
     7621 ccaataatca aaaacttgtg cttctgcttg ttttacagaa ctgtctacca atggcttaaa
     7681 tttcgccatg taaatcaccc tttcctacta aatattataa ttccaaaatt ttttattctt
     7741 tcacataaat ttaaatacta aaaaactcgc cccctttaaa ccaaagggac gagttataaa
     7801 tcgcgttacc accctaattc tatatgtaaa ctaaatacta gctacatata acactctaat
     7861 agtataacgg tactaaccgg cacagcttag tagaatttaa tcgttcagcc tgcagctcaa
     7921 gagtgatttt tatctttata aattcattgg ttctcagcat acccaattct ctgtaaaatt
     7981 atctaaagat aaatgtctct atcaacgctt ttaaaaattt atctgtgttt atatgaaatt
     8041 ctataacaaa taatttgaat agtcaatata tttttgaaaa atataataat ttctattttt
     8101 cttacatttt tatcctattt ttaatctttg ttatagattt ctcaaactca cacaattatt
     8161 ctacataaaa aataaacaaa ttacaagctt attctaaagt tgtagcagct tcatcattac
     8221 tataattata cactgttgtt ccttcaaatg catttacaca tttttcatct acatcataaa
     8281 aaatctcatc aacacttctt atttcatctt ctaacagtga tttaaattta tttctaaata
     8341 tcttaaattc tttgactaaa gcatcatact cttttcttat ctctataaca cggttattag
     8401 cttgttctat aatttgtcta gcttttaaat cagcttcttc aacaattatc ttagcctttt
     8461 tattagccgc gctacaagtg tcttctgctg cactttgagc agttattaaa gtagctttta
     8521 gtgtttcttc aatattttca tacttgttta cttggtcttg atataaccct aatttttctt
     8581 taagagctgt attttcccta catagagatt cataatcttc cttgatgata tctaaaaatt
     8641 catcaacctc ttcatctcta taccctctta aacctttttt aaactcttta ttttctatct
     8701 caattggagt tagcattaca tttcctccca gctataattt aaattagtcc ttatacaatt
     8761 aatcttgctt gaacttttat ttttcctttt ttagtaatat ctcctatttg tgttacctta
     8821 gctcttcctt tgcctctaat agaaatcaaa tcatcgtttt ttactatttt agaagttgaa
     8881 gttatctttt cataattgac atggactttt tctccattta tgtattttgc actatcttgt
     8941 ctagatatat tgtaaatacc acttatcaca caatctaacc tatctgaaga tactgtaaat
     9001 gatacttctt tatattttga agaactatta acaatatttt ctctggaaat ttcctttaca
     9061 attacattat ttcttgaaac cttttctaag tttacaatta taaaatcgca aatatcacta
     9121 cttacaacta cttgacaaaa gctatcatgt ataattatat ctccaatttt ttctcttttt
     9181 atacctagac ccagtattga ccctaagtaa tctttatgag atatattttt aaatttgaaa
     9241 ttcccttcta tttgtaaaaa tctaagagta tcttctatat cttcatattc catgtaaaat
     9301 ggataaataa atacagtgct tctttcagct tgttcataac ctccatccac actgtatttt
     9361 atttcatttg tagaatttaa tattgctaca gcatttttca cttcatatgg attaagaaac
     9421 tccgtagact taacatcata gttttttata caactattag ccttgtctat cactttaaac
     9481 attttatttt tcaagtctat atcctttatg tgatttgtta gttttaattt atccatccta
     9541 tcaccttaga atataaccaa tactaattgt cttaggaaca tcaataatac tattgcaatt
     9601 aaaggagaaa aatctatagg tccatttgta tatttataca ttatagacct tattggccct
     9661 tctattggct ctgtaagatt ttccaaaata tcaaaaattt tactttgtct tccatttgga
     9721 aaccaagtca tcaagctttt tataactatc atccatgcta ttatatctaa aagataataa
     9781 agtgcaattc ttattgttcc catatattaa ccgccttttt actatttatt ttgccaaggg
     9841 aagaatgctt tactttctaa ttcttttttc atatttgcat ctatatccac attattagga
     9901 gctagtataa atattgattt agaaaccttt tgtatacttc catctaagac ataaactgcc
     9961 ccattcataa agttaaatat agatttacga acttcagaat tagtcaatcc ttccaaattc
    10021 actataactg cccttctttg ttttagatgg tctgctatag ttgcagcttc atcataaacc
    10081 ttaggttcaa ctattacaac tttcatttgg ctagttgtat gtaaatttac aattttattt
    10141 gcttttgcag tactaaagcc actgttcatt tcttcttctt gaactatatc gtccatgcca
    10201 ctttcatatt catcttcaat gtaatcttct tcttcatcaa ctatccaatt cttaaattta
    10261 gatattattc catttgacat tattaacttc ctccttaaaa tttattcaaa tttgagtaat
    10321 tattttttat tgtagtttct ttctccaaag attgatgttc caactctaac aatggtagcc
    10381 ccttcctcta tagcaatttc ataatcgtga ctcattccca ttgacaaaac atccatccct
    10441 acattaggga tgtttagttc ttttatttgt tttgataaat ctttaagacc tttaaataca
    10501 cctcttatct catcttcatc ttctataaaa ggtgccattg tcattagacc ctttattttt
    10561 atatttttat atttttctga cacttctttg acaaagtcta caacttcttc tctatcaagt
    10621 ccatgcttag actcttcttt agatatattc acttgtacta agcaatctat ttctctgttt
    10681 atttgttctg cacgtttttg aatttcttcg cataatgcaa ttctatctaa agagtgtatc
    10741 atacaaactt tatcgattat gtacttaact ttattggttt gcaaagttcc tatcaggtgc
    10801 cagtttaatt tatcacctat aatatcatac tttctagcta attcttgggg tttattttca
    10861 ccaacagaag tgattccttc cctcattgcc tcattaacta agtctacatc aacagtcttt
    10921 gtgactgcca aaagggtaat ctctttgctg tctctatcta ctttttttgc agctttatca
    10981 attttgtttc ttatctcaga caaattatct ttaatagaac ccattattat cacctacttt
    11041 atccttatat ttgtgtttat actgttaggt cttaaaatta ttttgtcata tagattaaca
    11101 cttgtaactc catttatgtc atttttataa taatccacaa atacagaaga ttcattctca
    11161 taagttattc cttctaatat gacaaatttt acactttgtg attgttcatc aattacatat
    11221 acaccacgtt tattgtcttt tgtttttata gaagttttag gtatttttaa acattccatc
    11281 tgattatata ttatatcaaa ttcttcgaca cttgtatcct gatttgcaac aatttggtta
    11341 cttattttta attttaccat tattttatta ttattttcat atattttgta aacttttgca
    11401 tctaattttt gctcatgata ttcaattatt acattttgat ttttttcaaa gttattagaa
    11461 tcttctttac tgatgtatgt agatagataa ttactctggt cattggcatc tttttctata
    11521 cttcctttta catgaatctt tgcatcaact ttttcatttt ttaacaccaa cgtatttaca
    11581 tttttgctaa ttatcattac taatatatta aatactaaat atataagaat accaagtatc
    11641 actaatctgg tatattttac ctttttcaaa gactctcccc ctataaaatt actttcatta
    11701 taagtacttc ttctaaaaaa tgtttattcc ttttttataa gctaatattt tacgttctaa
    11761 taattatttt ttccatatta aatttatttt ttatcaatct tcctatacaa gactgcatta
    11821 tttatatata attgtattat aaagtataag tttaatttac attttatacg ttaagcttac
    11881 tttatatgtt tttatagtta tttttatttt tagatacaat ataaaaccaa ttaagattta
    11941 aaataacttt tgttatgttg acatttattt aggtaatcat ttatgaatta ggataacgtt
    12001 atcataacaa aagtttatgt cctaaaaaaa atacaaggag gattttctta tgtcagaaat
    12061 tttattagga acaggtttac ttttattggt tctatgttgt ttcacaattt ttagttacaa
    12121 ggccccatat ggtatgaagg ctatgggagc tctagcaaac gctgcatgtg ctactttctt
    12181 agtagaggca ttcaaccttt ctttacttgg tgatgtttta ggtatggaat tttttagaag
    12241 tgttggagct gctaatggta gtcttggtgg agttgctgct gctatacttg tgcctttagc
    12301 cttaggtgta tctccagttt atgcagtttt aataggatta tcttgtttag atttttctat
    12361 attatctgga ttcgttgctg gatactttgt ttctttctta attaagttta tagagaaaaa
    12421 agtgccacct ggagtggatt tagtggttgt aattcttcta tgtgcaccac tatctcgtct
    12481 tatagcatca aatgtagacc cattagttaa tagtacacta ctacaaattg gacaagtttt
    12541 gatagaagct accaacatta gccctataat aatgggaatt atactaggag gtctaataac
    12601 agtagttgga acgtctccac ttagttcaat ggctcttaca tctataattg gtcttactgg
    12661 tcttcctatg gcaataggag cattaggtgc ttttggttca gcttttatga actcaacact
    12721 atttgcaaaa ttgaaattag gttctaaaaa agatattata tctgttgcta ttgaaccatt
    12781 aacccaatca gacataattt cagctaatcc aatacctata tattctacta atttctttgg
    12841 aggagccttt tctggtatca ttgtagcctt gatgggtctt gtaaacatga caccaggtac
    12901 agctacacct gtggctggtt ttgctataat gtttgcttat aatccagttc aacaagttct
    12961 tataacagct ggtttatgtg ctttattcaa tgtaattgca ggatttttag gttcaaaaat
    13021 attcagtacc tttaaagttc ttactgctgg tgaaatacgt ggtactgatg tagaagaaga
    13081 aattgcttaa aaaacaataa gatttataaa tttatatctc aagtagtaga gttttatatt
    13141 gtaggcaatt ttattaattg caaacgtttt attttttatt tatgtttatg attattatgt
    13201 gtgttgaata tttagataaa tttttattta ttatttttat ttagattttt gtttagtttt
    13261 ttatttggtt gatatattag gaataaaaat tagtatagtt tattactagt tagatagcat
    13321 aactttttag atacataaaa aattttatat taattagata ttattaaaat aaaagcgata
    13381 catgaatttt taaatgtcca tgtatcgctt tatttttgcc ataatcttaa caactaattt
    13441 ttaaagttta ttatcttaac aaagttcatt atcttatata atatttaaat gcatagactt
    13501 ctttaattta accatctaaa tatttaatta aatattatta tatgtaatat aactaatttt
    13561 taaatgaatg tataggagct ggaatccttc ctcctctttg agtaaataat tcagatgaat
    13621 ttgtatttat aggcattact ggtgctcgtc ctaaaagtcc tccaaattct accatatcac
    13681 ctacatcaca tcctggggct ggtataattc taacggctgt agtcttatta ttaatcatac
    13741 ctattgctgc ctcatcagct atcatggctc caagcgtacc ggctgtagta tttcctggca
    13801 cagctatcat atcaagacct acagaacaaa tagcagtcat tgcttcaagc ttatctatac
    13861 ttaaagcacc tttaataaca gcatcaatca tacctgcatc ttcagatact ggtataaacg
    13921 ctccactaag tcctccaaca tgtgaacatg ccattactcc acctttttta acagcatcat
    13981 ttaaaagtgc taatgctgct gtagtcccat gagtaccaac aacttcaaga cccatttcct
    14041 ctaatatatt tgctaccgaa tcaccaatag caggtgtagg agctagtgat aagtctatta
    14101 tcccaaatgg aacatccaat ctactagaag cttcctttgc cacaagttgt ccaactcttg
    14161 taattttaaa tgcagtcttt ttaactgttt ctgaaacaac atcaaaaggc tctcccttta
    14221 ctttctctaa agctctctta acaacaccag ggccacttac accaacattt ataattctat
    14281 cagcctcacc aacaccatgg aaagctcctg ccataaatgg attatcttct actgcattac
    14341 aaaatactac taattttgca caaccaaaac cttttgcatc ttttgttaat tctgcagttt
    14401 cttttatgat ttctcccatt tctttcactg catccatatt tataccacat cttgtagaac
    14461 ctacatttac tgaagcacat actttattag tacttgctag agcttttgga attgacttaa
    14521 ttagtatctt atcacctttt gtatatccct tttgaactaa tgctgagaat ccaccaataa
    14581 aatctatacc taaatcctct gcagccttat ccaatgtctg tgcaaacttt acatagtcat
    14641 tttcatctgt tgcacctgca ataatggaaa ttggagttac tgataccctt ttatttacaa
    14701 ttgggatacc aaattcacgc tctatatctt ttccaacctt caccaaatct ttagcagaac
    14761 tcataatctt attgtatatt ttttctctag ctttatctcc atcagggtct atacaatcta
    14821 ataaagaaat ccccatagtt atagttctta tatccaactg ttcttcatct atcatcttta
    14881 ttgtttcaag tatatttcct acattcacta ttatcacctc cccttactgt ctactacaga
    14941 ttatgcattg aattaaatat ttcttctcgc tgaattttta catccactga taatttctta
    15001 cctctttcaa ccaagtcctc aaccgttttc tcaaaagaat ttaatgattt ctctaaatca
    15061 actatcataa tcatagtaaa ataatcctgt aatatttttt gtgatatatc tactatgttg
    15121 atattttgct tgtataattc atcactaatt cctgccacta tacctggttt atcctttcca
    15181 attactgtta aaattgcttt cataataata tcctccctta tttttttata aatcttatat
    15241 ttttacaaat tgaactatgt gttaacatat atttcattta tttgttagta aaccactttt
    15301 tatgtatata tttaatatat tttaatacct atacattcat ttgtcaattt tttttcaata
    15361 ttctattatt tttatactat ttagaaaatt catatattat atatgttttc tcaacaataa
    15421 atacagtttt cataataatg aaaggtatta cttagcaatc tcatgatttt ccaaataata
    15481 cctttctaaa aattattttt ttaattttat gaatttaatt atactttttc tagtatcagg
    15541 atttacaatt aatatagaac caatcataat tggtgttagt atacaaaata aaaaaataat
    15601 atcttttttt agattatatt ctttagattc taatttataa ttattattta ttattccatc
    15661 ataatcactt atatttgact tatttttatt tgtatcttga tttttgttag aaatactttc
    15721 cctataagga aaagaatgtt cattggtacc aaatactgat tttgagctta aatcataatt
    15781 atcatcattt tctgtaattg aatttttatt gtaaaatcct ttttctttat cagaattatt
    15841 ttctttatta ccgtctttag gtatatcatg aggattttta ttttcattgc tatcagtact
    15901 tgattctaca tcatctttag gggtatttgg ttgtttctca tcagaattat cattttcatt
    15961 attattattt tcattattat tgttttgatt gttattacta tcattatttt catttccatt
    16021 attcccatct tgattgttat catcctcatc tctatccggg tcgtctctac ctcctcctcc
    16081 acggtcacca tcaatatcat cattttcccg ttcatcatct ggtgtctctt ggttgtctcc
    16141 gcctcccatt ataaatcctg gtttaaaaac tatatttgaa tacccttctt ttaatcctcc
    16201 taccactttt actctaaaac aacgcaactt aaaatcttca aaaaaaagta tagcttgatc
    16261 tctaatatcc tcctgttctt cagatatcca attgattcca tcgcttgaat attcaacaaa
    16321 aaccttactt gcactatacg gcatatcata aaataaaaca gctacatatc cattttttgt
    16381 attttgaaat tccagttcca aattgtctat tggcttttta tctataacag atacattcaa
    16441 aataggaact acatcatcat ttattataat tttttcttta tttaacattt ctgttttaaa
    16501 ctttcctgtt atattaaaat cttctccctt ttccaacttt tcataataat cagtaaaatc
    16561 ccattctaca tctatatcaa gtcgattacc actagatgtt tctaatacca tatatttggg
    16621 aaattttata ttttctattt tatcatcttt acttactttt atttcatcac taaatacacc
    16681 aattccccta tgacaacatt tcacaatatc atagccagaa tcttcggcta tagagggtat
    16741 aagctcatta taatcatcaa ttatataaac tttcttactt tctgatataa ttgattgtgc
    16801 caacttatta ttatctctta gtaacccact ttcattgttg ctactatatg cttttattga
    16861 agtttcttct atctcaacat cacctttaga ataaacacct atagcatgtt ctccattcac
    16921 ttcaatattc ttatttagta ttctaatatc attttcagta taaattccta ttgcatcttt
    16981 tccatttgcc tttatttttt caacagattc gtaggatgat ttaatatgta agtctcctcc
    17041 ttcttcaacg tataaggctg tcccatcagt agcaattatg ttgtttatac caatacttat
    17101 acttccacca ttttctatat gtattactcc ttcttttcct ccttcaccta ttatattcaa
    17161 ttcattatca tcaataataa atctcccatg attttttatc aatattttat atttgttggt
    17221 atctattgta aaattactgt cataaggaat ataaaatcta aactcttcat ctattaccat
    17281 atcacaagaa agagtataaa ctatttcttc tttattattt ttattttctt gaacccacct
    17341 ttcaaaatct tcaagacaat tttcctcttg tccaaaaact ttatgtggaa aacttataaa
    17401 acacaaaact attatactca taaaaataaa tatctcatac tttttcatct tacactccta
    17461 gaaattttta gctttgaaac aatcctgtcg aaacatatac ttcacccact ataatttttc
    17521 cacaattatc aattattgtc aatttatata agtatgttta ttaattgtta taaataaaaa
    17581 gctacttact aaagcaaaca gacgtttcac tttaataagt agctatattt ttataacaaa
    17641 atagttataa tattagaact tccttcattt acaatttttt gtaaagtctt ttgcatttta
    17701 actcttatct cttctggcat agtatataat ttactttgta attgctcttt tactaagtca
    17761 tgtagtgact taccaaacat atttgattcc cataaatctg ctggttgctc ttcaaatact
    17821 tccataagat attttatcat ctcttcacct tgattttgat ttccaactat tggagaaacc
    17881 tctgtagata tatctgcttt tataatatga agtgatggtg cattggcttt taacttgata
    17941 ccatattgtt ttccttgttt cattatttct ggttcttcca gactaagttc ttctaaggaa
    18001 ggagctacta ctccatatcc ttttatcttg gcatctatta aagcactttc tattttatca
    18061 tactcatttt taactttaga tagtctagtt atcagactaa gcagttgata atctccttca
    18121 attttaaatc cacttttttc ttcaaggaca ttatagaata aatcttgctt tgtctgtaaa
    18181 tcaatattta taacaccttc tcctagctct acattatcaa cacctgtatc ttccaaaaac
    18241 tctaactcag aaaatccttg tataatacct tctatatccc ttattttacc tatatctata
    18301 atactttgtt ttaatgttgt tattatgcta ctttttatcc aatgatttct ttcaagacct
    18361 tcaacccatt ttggtaagtt gattcttatt tctgtaagtg gaaaatcata taatacagct
    18421 tccataacct cctctatatc ttcttcttcc atttcaacca cattcattgg aagaactgga
    18481 acttcatact tttcttccaa ttcacttcta agcatactcg tttcctcact ttttggactt
    18541 aacgtattta aaaccactgc aaatggtttt ttaagatttt tcaattcttg tataactctc
    18601 tcttctggtt ctacatagct ttttctgtca attccagtaa ctgaaccatc tgtcaagaca
    18661 actatcccta ttgtagaatg gtcttttata acttttttag ttcctatctc tgctgccttt
    18721 tcaaatgtca tagcttcttg tgaccaagga gtggatacta atctttgttt tccaccttcc
    18781 tcatgcccta aagctccttc aactatgtat ccaacacaat ctaccattct tacttttaaa
    18841 gatactgtgt cttttatttt tatttctaca ccatctgctg gtacaaattt tggttctact
    18901 gtcattatcg ttttacctga accactttgg ggtatctcat ctcttgttct atccttttta
    18961 aattcattgt ctatattagg tataaccaac ttttccataa attttcttat aaaagttgat
    19021 tttcctgttc ttacaggtcc aactactcca atatatatat ccccctgagt ccttttggat
    19081 atatcttcgt atatgttatt attcatatta ttcctcctat acatctaatc ttattaaatt
    19141 atatttagag ttaagggtta atattcttaa aatagaaaaa taaagaccag caatattaat
    19201 ttaatttaca tatagaaatt tgtaaatttc aatgtaaatt aaattaatat tgctggtcca
    19261 aatagtagtg tattatatta actgacttta gtagtatttt catttgaatt attattcatc
    19321 tcaataagtt ctttacgtct ttcttttgtc atgttcatcc atataaatgt aaacaccaat
    19381 ccaattatgg ctactatagt aagaactatg aagtagtacc tatatccagt tacacccgga
    19441 aatgcatcca atatcttccc tgctgctaaa ggacaaagta cttctggaag atatcctaat
    19501 gtagcaacta ttcctatagc agttcctgat attgataatg aataattacc ttcttcaagt
    19561 aacgaatagt gtaaaccttg aattgcatac atagatacat atattgcaac acatccaatc
    19621 aatagtatat aaatcataga tgattttgtt ggtgtaaata ttacacctaa aagacctgca
    19681 atcataagta taaatccata agatataatc tttgatgaac caactttatc tccaagtaat
    19741 ccagaagcag ctgatgctac tggtctacaa tactgagcta atattgagat tgcagatgca
    19801 aacaccacag tagctccaaa tgcttcagtt gaataaggcg taaaataata aaatgacata
    19861 ttagctgaat atgaacaaaa cattattatt gttattatcc atgtatgagg catttttatt
    19921 actgcaaaaa aggcttcttt atcaaatttt gctgatacct ctttttcatt atcctttaat
    19981 ttgaaaaata ctaatactcc aactattaca tttattgccg agtataatac tataatagct
    20041 gtaagaccag atttatcact tacttttgat gaaatatacc caaatagtat caatactaga
    20101 gatagatgaa ttgcattggt aattccacga ccactttcca taaatccaaa agcttttcct
    20161 tgttcatttt cagatgctaa cattctaaca gcttttacaa gagctggcca aaaagtaagt
    20221 attgttgtta ttccccataa ggcatggatt aaaaacacaa caatatatgg aggataaagt
    20281 gttaatacaa gaccagatat tcctgtaata attaaagaac ctgaaagcaa ttttctggct
    20341 gaaatacggt ctgctaaaaa accaccaaaa aggtatgata ccattgcaag tagaccataa
    20401 atacttccta agcttcccat ttgtgtattt gtaagattaa atgcttgtgc aaaagtatca
    20461 taataataat tctttaaata tggtaaagca tatataaatg ctcctgctac aaagagtatt
    20521 atcaagactt tgaaattttt ccaaatgctt ggtttattta cttcaactga catacttcct
    20581 ccttatgtaa atacctgtaa attttcatga taattaatta ttgcttattg tattgtttat
    20641 tcaatatttc ggaatattta ttaatatatt aggttaatct atcaataatt tatggtgagt
    20701 tatgagctaa aagtaaactc ttttagctca ccataataat tatattttta tttatactaa
    20761 cgtccagagt tattagatat ctttttttca tagttcttcc atagcattaa acctgttatt
    20821 ataacaaaaa gacctcctcc tatcatttca atctcataga ttatattatc ttttaaagtc
    20881 tccataactg gtgccgttga gaaaagtatg ccaactacag ttactacaag taccattctg
    20941 gcaatattga tacaaactga atcacttttt gcaattgaat atggtctttc ttcatttggt
    21001 cttgtctttc taagtctaat ataagatgta aaaagcaaga catatggaaa taatgctgtt
    21061 aatgctgtca tagtaacaag aacattgtat atattatcta ctgatggtaa taagtttgtt
    21121 cctacaagaa gtagactcac tattatagcc tgaactatta ctgcatttga tggtatattg
    21181 tatttatttg taacagtcaa ttgcttagga aatacacctt ctcttacatt tccaaaaagc
    21241 atctttactg gacctgctat ataaagtatt aatgctccta gtactgataa agttattcca
    21301 aatgctacta ttcttataaa ccaacttcct atacctaaat cttgtgcaac tttagctaat
    21361 gcatctagta tccctgtaga tgctgcaatt ttatctggag gaagtatcat tgttattgct
    21421 actgaaccta agatatagat tcctcctatt aaaacagcca cagttaatat agctttagga
    21481 aagtttttct tagcattatc catttcggtt atgaaattag cagtcgtttc agctcctgat
    21541 aatgcaaata ttatagcaga tatagaaact aatgaattgg catctatttt tggtattata
    21601 ttttggattg tatatatcga tgctgatgga gcttttttga gaataactac agacataaat
    21661 gccatcacta taagtaatat agcaggaatt gtagaaccta atgctcctgt attcgtaaaa
    21721 aattttccaa atgccatccc acgagtactt actaaggaca acagccaaaa tattatgagt
    21781 gaaagtgcta aaataaacat cttattgttt gataaatctg gatttccaag catatatgca
    21841 atatttatcg ctaaaaaagt caagaaagaa gaataccaaa atatctttga tgtccaattt
    21901 agccatgaca ccataaatcc ccacttctct ccataagctt gtttaaccca ttctccaagt
    21961 ccaccatctt ttggatatgt tgcagccaac tctgcacata taagtgatgc aggaacaaag
    22021 aatataaaag caaacaataa ccaaactggt atagaaccaa gacccaatcc aaaactagag
    22081 gcagtagatt tagctatcca tctaatccca aatagtgctg atatattcat aaatactaaa
    22141 tcccaaaatc caattttctt tttcactgta attcctccaa tttcaaattt attcagactc
    22201 atacacaatg tattgactta aatatcaagt ataagttctt caacacttct tggataatta
    22261 gttataattg tagcaccatt cttacctact attgctgtat ctgagtgtct aaatcctcca
    22321 actccaggta tatatatccc tggctccata gaaaatacca ttccttcttc taatattaat
    22381 tcattgtcaa atcttaaata tggctcttca tgttctgata aacctaaacc atgaccaatt
    22441 ctatgatttg aatataactc tagaccatac tttgcaacaa cagctcttgc tgcttcatcc
    22501 accattcttg ctggtatacc agcttttatt gtatctaatc cagcttgttg agcctctact
    22561 gcaatcttaa acacttcttt ttgtctttct gttggtttgc ctataataaa agttctttca
    22621 ttttctgctc tatagttttc ataccaaacc tgtctactat gtattactat atctcctctt
    22681 tgaagtattc tagtattgga atctaaatga ggttgtgctg ttctatcaat ccctgaacaa
    22741 gtccagtttg caaatcctat atatgtatca ggataatttt cagaaacata ttttagtaaa
    22801 gcattatccc ctgcaacgtc aaactccaat tcactcatcc caactctaac attttctaaa
    22861 gaccctttta ttcctatatc agataggtaa cctgctatct tcaaaaattc tatttctttt
    22921 gcatctttta catatctcat ttcaaatatt ttacttccaa catctttgat agcaaatgac
    22981 ttatcagtta tatactctga aaagcttgca gatactatat ctttttctat tccaacagtt
    23041 gttcctttag ggtatttatt taaaattata tctaagtaat gtttatgtga tattccatgt
    23101 tctttcattt ctggtatttc ataatacaca tatacattat caacttttgc aacttcttta
    23161 gcatgtaatt cttctaatcc aggtactatt aattctattt tatcttgagt tactactgtt
    23221 acaattggtc tagaataagt tattgcttta aatccactaa tataaaattg attgtctggt
    23281 tcaaatatta aagcaatttc aatgcttttt tcattcatgt actttctcaa tctttctagt
    23341 ctcatattat ttgacataat gcacctcttt cttaatattc agataattta ttcaatacaa
    23401 caaggatgaa tatattattt taaaattctt aatattttta gtacttgtcg tatttgtatg
    23461 taaattaatt ttatttgtga ttattttatc ataatcatga ttattttgtc aatcatgatt
    23521 ttattttaaa aaatttttta tattctagtg attttaaatt atctaaaata
//
LOCUS       DAGSHT010000038        23439 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGSHT010000038 DAGSHT010000000
VERSION     DAGSHT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.039"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     regulatory      <1..63
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            252..542
                     /gene="fliE"
                     /locus_tag="MJB72_001379"
     CDS             252..542
                     /gene="fliE"
                     /locus_tag="MJB72_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBY8262500.1"
                     /translation="MQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTIDKVNFQQVT
                     ADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNKVQI"
     gene            567..2117
                     /gene="fliF"
                     /locus_tag="MJB72_001380"
     CDS             567..2117
                     /gene="fliF"
                     /locus_tag="MJB72_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBY8262501.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2130..3200
                     /gene="fliG"
                     /locus_tag="MJB72_001381"
     CDS             2130..3200
                     /gene="fliG"
                     /locus_tag="MJB72_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBY8262502.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3193..3924
                     /locus_tag="MJB72_001382"
     CDS             3193..3924
                     /locus_tag="MJB72_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBY8262503.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            3928..5247
                     /gene="fliI"
                     /locus_tag="MJB72_001383"
     CDS             3928..5247
                     /gene="fliI"
                     /locus_tag="MJB72_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBY8262504.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5279..5725
                     /gene="fliJ"
                     /locus_tag="MJB72_001384"
     CDS             5279..5725
                     /gene="fliJ"
                     /locus_tag="MJB72_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBY8262505.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            5753..6958
                     /locus_tag="MJB72_001385"
     CDS             5753..6958
                     /locus_tag="MJB72_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBY8262506.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            6976..7584
                     /locus_tag="MJB72_001386"
     CDS             6976..7584
                     /locus_tag="MJB72_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBY8262507.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            7611..8594
                     /locus_tag="MJB72_001387"
     CDS             7611..8594
                     /locus_tag="MJB72_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBY8262508.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            8607..8810
                     /locus_tag="MJB72_001388"
     CDS             8607..8810
                     /locus_tag="MJB72_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBY8262509.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            8810..9628
                     /locus_tag="MJB72_001389"
     CDS             8810..9628
                     /locus_tag="MJB72_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBY8262510.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            9618..10313
                     /locus_tag="MJB72_001390"
     CDS             9618..10313
                     /locus_tag="MJB72_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY8262511.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10325..10768
                     /locus_tag="MJB72_001391"
     CDS             10325..10768
                     /locus_tag="MJB72_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBY8262512.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            10783..11157
                     /locus_tag="MJB72_001392"
     CDS             10783..11157
                     /locus_tag="MJB72_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBY8262513.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11158..11823
                     /gene="fliP"
                     /locus_tag="MJB72_001393"
     CDS             11158..11823
                     /gene="fliP"
                     /locus_tag="MJB72_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBY8262514.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            11836..12105
                     /gene="fliQ"
                     /locus_tag="MJB72_001394"
     CDS             11836..12105
                     /gene="fliQ"
                     /locus_tag="MJB72_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBY8262515.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12117..13931
                     /locus_tag="MJB72_001395"
     CDS             12117..13931
                     /locus_tag="MJB72_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBY8262516.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMIDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            13940..16015
                     /gene="flhA"
                     /locus_tag="MJB72_001396"
     CDS             13940..16015
                     /gene="flhA"
                     /locus_tag="MJB72_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBY8262517.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            15990..17006
                     /gene="flhF"
                     /locus_tag="MJB72_001397"
     CDS             15990..17006
                     /gene="flhF"
                     /locus_tag="MJB72_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBY8262518.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17003..17881
                     /locus_tag="MJB72_001398"
     CDS             17003..17881
                     /locus_tag="MJB72_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBY8262519.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            17895..18596
                     /locus_tag="MJB72_001399"
     CDS             17895..18596
                     /locus_tag="MJB72_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBY8262520.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            18612..19034
                     /locus_tag="MJB72_001400"
     CDS             18612..19034
                     /locus_tag="MJB72_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBY8262521.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            19019..19204
                     /locus_tag="MJB72_001401"
     CDS             19019..19204
                     /locus_tag="MJB72_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBY8262522.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19245..20018
                     /gene="flgG"
                     /locus_tag="MJB72_001402"
     CDS             19245..20018
                     /gene="flgG"
                     /locus_tag="MJB72_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBY8262523.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20039..20800
                     /gene="flgG"
                     /locus_tag="MJB72_001403"
     CDS             20039..20800
                     /gene="flgG"
                     /locus_tag="MJB72_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBY8262524.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            20823..21704
                     /locus_tag="MJB72_001404"
     CDS             20823..21704
                     /locus_tag="MJB72_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY8262525.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            21707..22081
                     /locus_tag="MJB72_001405"
     CDS             21707..22081
                     /locus_tag="MJB72_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY8262526.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22113..>23439
                     /locus_tag="MJB72_001406"
     CDS             22113..>23439
                     /locus_tag="MJB72_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262527.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLT"
BASE COUNT         9684 a         2521 c         4389 g         6845 t
ORIGIN      
        1 tagtgacgca aagctatagg gactaaggtt tatatacata aactatgtca gccagttgcc
       61 aaaaagagtc ctaggtgtat tgtataccta agaaaagtct ataatgactg cctttttggc
      121 agtcattttg ttatggaaaa atcactttat ttcagtagaa aatacacata ataacatatt
      181 ttaccaatta taacataaaa taaagaaaaa ttatctcaaa aagttactat tttacacaaa
      241 ttaacaattt aatgcaaggt gcaaagttag ataatttgat tggagaagca aagccattaa
      301 ctcttaataa taaagaagaa aaaaagaatt ttgaagatgt aataatagat actatagata
      361 aagttaattt tcaacaagta acagcagatg atatgaaaga aaaatttata aaaggcgaag
      421 atgtttctat gcatgaagtt atgttaatag gtcaagaagc acaactatct atgcaatatt
      481 taatagaagt tagaaataag attcatgatg catatcaaga aatgaataag gtgcaaattt
      541 agccttttga acaggagtaa taatcaatga taaccagttt taaaaataag attaaggagc
      601 aagttgctaa atctaggaca aagataagtt ctttaaaaaa gggaactaaa atagcattag
      661 gtataggggt tattgctata tgtttagcgc tagtatttac agcaatatct gcgagcaaaa
      721 ataagtatgc agtcttattt tcagaactag atgctcaaga tgctgcagat atatcagcag
      781 agctagagtc taaaaagata gatactaagg taaaaggaag tacgatttat gtacctagaa
      841 cagaagttga taagttaagg ctggagttag cgccaagtct tacaggtggt tctaaaggat
      901 ttgcactaat ggatgatagt tcatcaatag ggcttactga tgaagaattt cagattaaga
      961 aacaaagaat gatacaaggt gaaatagaaa aaacaataaa gagttttcca caagtggaag
     1021 acactagagt acatataaca tttggagagt catcagtatt tgagaaggag gctgttccag
     1081 ggagtgcagc agtttatttg atgctaaaac cagggacgac tttagaaaaa aaccaaataa
     1141 agtctgttat ggcacttata tcaggaagcg ttacaaatat accagagaaa aatgttcagg
     1201 ttattgataa caaaatgaat ttattatctg aaggtatttt ggaggatgaa aatgttgatg
     1261 aaaataatca accaataaat agtggcatta gtgttgataa aaataaggca tctgaacaag
     1321 cattgaataa acaactagaa aagtctattt tagaaatgct agaaccgata tttggtaagg
     1381 gaaaggtgaa agctacagta aatgctgatt taagttttga tacagctgaa aaaactgaga
     1441 taaaaattga ccctgataaa gttgcaataa aggagacaag gtcgaaaaat tcatctaaat
     1501 ctgctgcaaa taatgttcaa ggtgtagatg ctaacatgaa taaccaagga aataacaatg
     1561 gtactaatac agaagatagt ttagatgaga gttttgagta tgaaacaggt aagattgaaa
     1621 ctcacactat agttgcgcct ggagaactta atagattgac agcttctgtt gcaatagatg
     1681 gaaaaatagc taaaggtgtt caagctgatg tagaggatat tgttaataat gctataggta
     1741 tggatggagc gaggggagat actcttagcg ttgtttcaat ggtatttgac ccagaaggta
     1801 agaaagaggc taaacaagag ctggaagaga ttaaaaaaga aggatttaaa aagaacataa
     1861 taaaagcagt tattggagta gtagcagtaa tagcagttgg agctattgga tacttaatat
     1921 ataagaaaag aagtgaacga gaagatatgg attatgaaga tgaagattat atttctaaat
     1981 ctgattctgt tgataggaag gataatcctc tattaggaga tgacccagaa ctgacattag
     2041 aggaagctgt taaaatgtat gcagaagaaa agccagacca agtaacagag ataataaaga
     2101 cttggttaaa tgagtaaggg ggatagaaag tgtccacgga aattacaaat gttaatgcgt
     2161 ttgacttaag tgatataaat gaaagtgata agaatgagaa ggtagaaggt gagggtttaa
     2221 aaaaagctgc tgtattatta atgacattag gcccaaatgt gtctagtgat ataataaaaa
     2281 gcttgcctga taaacaagtt caaaaaatag gtttggaaat tgcaaatatt tcttcaatag
     2341 cttcaaaaga aagaagagaa atcttaaaag agtttgtaga aataaatagg gcaaaagatt
     2401 atgttataga aggtgggctt gaatatgcaa gagagttatt aagtaatgct ttaggaaatg
     2461 ttaaagcaaa caaattactt gaaggtatat caataaatac aagtacaaag ccttttaaac
     2521 atattataaa actagatgta aagaagatat tagaaatact taggaatgaa agtgcacaaa
     2581 ctatagccgt tttacttgca catatggaac cagaaaatgg agcagaagtt ttatcatact
     2641 tagattttga tttacaaaat gaagtagcta tgagaataag ctcaatgggt tcaatatctc
     2701 cagaagtaat aaaggtaatt gaaagttcat tagaagataa attacattca ataagtggtg
     2761 atgattttaa tgattatgga ggggtgtcta ccttagttca aatattgatt aattctgata
     2821 gaaaaacaga aaagaatatt ttgggaacta ttgaagaaca aaacccggat ttagcagctc
     2881 aaattaaatc tcatatattt gtctttgaag atattgtcaa gttagatgat ataagcatac
     2941 aaaaagtgtt aaaagaagtg actatgaaag atttagtata tgccttaaaa ggtgtagatg
     3001 atgagatttc agatgctata tatagaaatc aatcttctag agcagcagat gcacttaaag
     3061 aagaaatgga aatggttggt gttataaagg ttacacaagt tgaggcttct caacaaaaga
     3121 tagctaatat cgtaagaaaa cttgaaagag aaggtgccat aactattgta aggaattttg
     3181 gtggtgactt tgttgaataa taagattatt aaaagtaaaa aagcattata taaaggagtg
     3241 ctagatttaa ccataataga taaagttaga gtgggagaat caaatgtaaa taaagaaaat
     3301 gattgtagat ttagggatga ggtaataaaa gaagctatag aatctgctga gataagaaag
     3361 aatgagatat tagaagaagc aaaaatgaat gcacaaaata tagaaaagca agcatatgaa
     3421 aaagggtata gtcaaggtca aaaaaatgga tatgaagatg gatataaaga gtcatatgat
     3481 gaatataccc aaaaagctaa agatgaagca gattttataa aaaatcaggc agacttaata
     3541 ctattacaag ctaatgagaa gatgactgag tacataaaag aaaaaaataa agagattgta
     3601 agacttgcta ttaatatggc tagttctgtt ctaaaaaagc atttcgaaga tgaagaatct
     3661 atgagtgatt tgttaaagca aattattcaa gattatgaag gaagtagttc atttatagta
     3721 agatgtaatt ctttctataa agataatata gagaaagagt ttgaattatt caaagaggaa
     3781 acatcactaa aatcaagtgt atttgttatt gcagatgata gtatagataa aggcaatgct
     3841 attatccaaa aggaaaatgg aagaataata gttggtattg attgtgctat ggaaaaacta
     3901 aaagaagaat tgcttggtga ataaagtatg tataaaactg atatatctat agattttgac
     3961 aaaataaata agaaaataga aaatttagaa ctcactatat cagaaggtag agtaaagaag
     4021 ataataggac ttactgtaga agttgaagga ataaaagctt ttgttgggga gttatgcgtt
     4081 atatacaacc aagttaataa accagtgaat tgtgaagtag taggttttaa agataaagaa
     4141 gtcatattaa tggcgttggg agaattaaca ttgatagcac ctggttgtaa agttatttct
     4201 aaggggatac ctttaagtgt tatgtgtagt gataatttac taggtaaagt acttgatggt
     4261 cttggaaatc ctatagacaa ctcagatgct gttttaggag atagatacaa cctgaacaat
     4321 gaacctcctg acccaatgaa gagaaaaaag atacgaaata taatggaaac aggtgtaaga
     4381 gcaatagatg catttactac ttgtggtgaa ggacagagaa tcggcatatt tgcaggaagt
     4441 ggagtaggta agagtacgac tctaggtatg attgctagga atgcaaaagc agatgtaaat
     4501 gtaattgctc ttattggaga aagaggaaga gaagtactag attttattga taaagattta
     4561 ggtgaagagg gaatgaaaaa atctgttgtt gtgtgtgcaa catcagataa agcccctcta
     4621 gttaggttaa aaggtgctct tactgctact gcaatagcag aatattttag agaccaaggc
     4681 aaaaaagtaa tacttatgat ggattcagta actagatttg ctatggcaca aagagaagtt
     4741 ggtgtagcaa taggagagcc accagctcaa aaaggatata caccatctgt atttgcaatg
     4801 ttgccaaaac ttatggagag aacagggaca tcagataaag gttctataac tgcattttat
     4861 acagtattag ttgatggaga tgactttaat gagccaatag cagatacaac aagagggata
     4921 cttgatggtc atatagtttt atctagagac ttggcaaata agaatcacta tccttctata
     4981 gatgtattaa acagtttaag taggcttatg aatgaaattg cttcaaaaga agacataaaa
     5041 atagcgtctt ttgcaagaga catgcttgct gagtatagag aagcagaaga tttgataaat
     5101 ataggtgcat atgcttcagg tacaaacaaa aaaatagatg aagcaatcta ttatcatgag
     5161 catattatta attttttaaa acaaggaata aatgaaaaga gttcttttaa tgaaactata
     5221 agtagtttaa gaagaatctt tgaataaaca atttaaaaaa atatttgaat aggttgtgat
     5281 ggaattggat aaaaatttta agtttagatt acagaaagtt ttagacttaa aaataaaaga
     5341 tgaagaagag ataaaaatgg aatttgctaa gattcaacaa aaaaaaatag atatagaaac
     5401 taatttagaa aacttagaaa gtaattacag caaatattct ataagtaaaa ataatgataa
     5461 tgttcaaaat caaaaaataa ctattaacta tttgctagct ttaaacaact ctataatgga
     5521 tttatcagaa gaactggata agtcgacaaa tgaacttgaa aaagctagaa aacagcttat
     5581 aagcaaacag atagagagaa agtcattaga aaaattaaaa gaaaaaaaat atggacagta
     5641 ttataaagaa gaacagttaa aagaacaaag cactaatgat gagtttgcaa gcatgagtta
     5701 tttgagaaat agacaagttc tataaaataa taggaagtga ggatattatt gtatgaagat
     5761 acaagcagat tcttataata ttattaaaga tattgggaat agaaaaaact catctaagaa
     5821 taaaaatata aaaaataata aatttgaaga taatctatat aagacaacag accataaaaa
     5881 tgttaaagat agaagagaaa caagtaagca aaatgttagt tcagatagac cgggaaatag
     5941 tgataatgag tgtcatttag atgaagataa aagtaatcac ttagaagatg aaaatacata
     6001 tgaaaaagat tgtagtaaga ataatgaagc tttacagcaa ttactgaatc ttttaaaaga
     6061 gaattataaa aaagataaag atataaacac tatattgaat aatataaaaa aactaagtat
     6121 atcagatgaa ttaaaacaag aacttaagct taataaaaat atattgaata aaattgcaaa
     6181 tcaaaattta gagttaaatg gagctaatac aaataaccct aatgatatag aaaatttaga
     6241 cataatgaag ttaagagaac ttttagaaaa taatgctagt gaagatattc aagtaattag
     6301 taaaactaca gaaaatgata ctaaaagcat agacttatac agttttgata gtaatagaat
     6361 ggatactctt aactcaaata aacagaaaga tgatagttct gatattttgg aaaaactagc
     6421 agggttaaat ggaaataaga gtggaaattt tggacaagta ataaataaaa gcgttgatat
     6481 gaataaaatt aaaaatgata atgttaattt aaaacagata gaatctaatg taatggatga
     6541 ttctataaaa gctattaagc atatgaaaac aaatgatata caagaattga caattaaatt
     6601 aagaccaaaa gaattaggag atatgaacat acaattatta aaagatggtg aaagtatgag
     6661 agctgttgtg actgttttca ataaagatgt ctttgactct ataaataaaa acatagcaga
     6721 tttaaagcaa catcttgaac ttacaaatgt aaatataaaa gatgtatcag tgcaaatgca
     6781 tagcgataat acaaatacat ctgatacctt tgataaagca tttgaacaac agaatagaca
     6841 aaataaccaa gaaaacatga gtaatcaaaa tacaaatgat aagcgtaaca atgtagtcat
     6901 agaagatgag attaaagaaa atattattga tgatgataga gtagatttac tagcttagta
     6961 agggggaagt attgcatgta cggaatagca acggctttgg caggaactca ggtaaactat
     7021 aggtcaaata acttaagcca acttgaaaga aatattaatg atattgccaa caaacaagtt
     7081 agaagtaaag gaaatcagac agtaacagac aatgggactc caattatagg acaaggtgat
     7141 gatgctgata aagacttgtt tatgaagatt cttgtagcac agatgggtaa tcaagaccca
     7201 atgaatccac aagacccaac ggagtatgta tcacaattag cacagtttgc aactttagaa
     7261 aaaatgtcag ctatgaatga taagctagat atacttttag ccttaagtaa ctctactcta
     7321 ataaactctg ctttgagtat ggcaacatca ttaattggta aagaagtaga agtatctggg
     7381 gaagaagatg aagatgaaaa cattgtagga gtagtaaagt ccacatacat aaaagatggg
     7441 gaagtacact tagaattaga agtagatgga agtgacaaaa ctgtagatgt taaatatgaa
     7501 aaattactta aaataggaga ggtagaggca aattcaggag aagaaaatga ctcttcagaa
     7561 caagaatctc cagaagaagt ataatattaa atgggaaaag aggtaaattt atgataaaag
     7621 caatgtattc aggtgtaagt ggaatgaaag caaatcaaac aaaactagat gtaatcggta
     7681 acaatgtagc caacgctgga actacttctt ttaagaaaag ttcagctaga ataacagatt
     7741 cattttatca aactgtacta tatgcaagtg ctccaacagc ggcattaggg ggaacaaact
     7801 taggacaagt aggtgtaggt agtaagatat caagtataaa caaagatatg acacaaggaa
     7861 atgtacaacc aactaataga ccatctgatt taatgataga tggagatgga tatcttccag
     7921 tagtaagaca aggaacagtt atgtatacga gagatggttc ttttaattta gatacaggat
     7981 ttaaagcagc tgatggtgct ttgaagaata ttaaaggtgg aagacttgta aatggtgatg
     8041 gatatatgtt acaaggtatg atttatgatg gggaatataa agctgatact ggagaatttg
     8101 ttaaaaaggg tgatgctaaa ggaaaagagc ctattgtaat accacttcaa agtataagtc
     8161 ctaaggatgg aacagttcaa aatgtactag agtataatat atcaaaggat ggaacagtag
     8221 aatttttatt gagtgatggt caaagaactc aatttgtcta tgatatacct gatggtgcta
     8281 aggtaggaga tacacctgct ggaatagata aattaaaagg tacaatacaa aaagtgcaaa
     8341 catatgcatt ccaaaatcca gcaggactag atgcagaagg aggaaacttg ttcaagccat
     8401 cagcaaactc aggagaccca caaatagcag gagctacagg cacaataata caaggagcta
     8461 tagaagcttc caatgtagat atagcagaag aatttacaga aatgataata gcaagtaggt
     8521 ctttccaagc aaacagtaaa ataatatcga catcagacga aatacttcaa gaaataataa
     8581 atcttaagag atagtaggta catattatga taagagtaac ggatttaaac ggaagaaaat
     8641 acatattaaa ttcagatctt atagtgagaa ttgactccgt gccagaaagc aggattctcc
     8701 ttacaacagg agaaaaaaat cttgtaaaag agtcagtaga tgagatagta gataaagtaa
     8761 taaagtataa aagaaaaata aatgtaggtt atattaggag tgaaaaataa tgaaaaaaac
     8821 agattcacta acattcattg gactaatatt atctacagta ttagttttat ttggagcagt
     8881 aaaaggaagt agttcaggat tgaaaagttt tttcgatttt gcatctatat taatcacagt
     8941 atttgggtca tttggagctt tgatgattac atttactatt gacgatataa aacttataaa
     9001 agatgctcta caatactcat tcaagacaat gaatatatcg aaattagact tattaaatca
     9061 atttaaaaca ctttctaaaa aagcaagaag agaaggactt ttatctatag aaagtgatgt
     9121 aatgaaaata caagacccat ttatgaaaaa aggattagag ttatgtattg atggtcttga
     9181 aataaatgaa ataaggtcta tactagagct cgaaatagat tctgttgaag aaaccaataa
     9241 cagatattct aaactattta aaacttgggg gactttcgca cctgcatttg gtatgttggg
     9301 aactctaata ggtttaatac aaatgttatc cgaccttaca tctccagatt taatagcatc
     9361 tggtatggga aaagcattaa ttacgacatt ttatgggtct ttacttgcca atatcgcttt
     9421 gaacccaatt gcatataata tagatgagaa gactgaaaaa gaaatttatg taaaggaaat
     9481 gatgctggag gggataataa gcatacaatc tggagagagc agcatagtag ttgaggaacg
     9541 tttagctaca tacctttcaa ataatgagaa gttagaaatt atgaaatcca ataaaaatac
     9601 agaaagagca atgagtaatg gcgcgtaaaa aaaataagaa aaaggatgag ataaatccag
     9661 atgcatggtt ggctacttat gcagatacga taacactgat tcttacattt tttgtgttat
     9721 tgtatgctac atcagttgta gatatggaaa aatttaaaaa tatagcagat gcgttaaaag
     9781 gtcagttttc tgggacaagt atgtttagta caggttcaag tacaagtgag aaatctccaa
     9841 tagagagtgt tttagtggat aaaaatgata agtcaaattt agaacaggat ttaaaagata
     9901 aagttaataa ttctaactta caaaatagtg taacagtcaa agatgatagt agagggattt
     9961 tactagagct agatgattca attttatttg actctgggat ttctgaatta aaagaagata
    10021 gtaaagccac tcttaataaa gtatacgata tgataaagat gatgaaaaat aagatagtta
    10081 tagaaggcca tactgataat gtacctgtaa aatcaagtac gcatgaatcc aattgggatt
    10141 tatcttctag tagagcagta agtgttgtaa gatactttgt agaagaaaag ggcatggacc
    10201 ctaaactatt ttcggcaaca ggatgtggag agtaccaacc tttggtatca aatgaaacag
    10261 caaagggaag agctgagaat agaagagtta atatacttat aactattgac taggaggaag
    10321 aggtttgttc aaaaataaaa aaattattat aattattttg atactgatta ttttctcagc
    10381 aggagcaggt ttttttatat taggagaagg tggaaataaa caaattgaca agattgcagg
    10441 tttgtttatt ccaacaggga aaggcaaaga tgaagtaaag accgaaaatc ttgacttaga
    10501 agagatggta ttgaagctag ctgatgaagg tgtaagatat ttaaaagtat caataactgt
    10561 atcttatgat tcttcatata aagatataga aaaaaatacg cctttaatta gggataatat
    10621 tataaattgt tttatggcaa aaagggcaga tgattttact gctgataatt taaagaacat
    10681 aaaagaagaa attaaaaata cattaaatat acaactaggt gaaaatgtaa ttcaaaatat
    10741 atatttcaca aatatagtag tgcaataaaa ggatggtaag acatgatact ttatttacta
    10801 aaaatattaa tatgtatccc aataatttta atattgatag tcttatcttt aaaaattagc
    10861 caaggaactt tattaaaaaa atataatgat aaatatataa aaatactaga tattgtaaat
    10921 ataagtaaaa acaatagcat aataatttta aaaataggtg aagaaggatg cattatggca
    10981 tcttcttctt ctagcatgag taaaataaaa gatttgacca aagaagaaat attaagaata
    11041 gaaaacgaca ttaaagaaag aaattttaaa attcaagatt ttaaaatgga aagaattaaa
    11101 aagaaatatg atttcagtag atatattgga aaatttacaa aaaaggaaga taagtaaatg
    11161 gaactattat caaaattacc tttggctgat gtaccatata caagttcaat gcaattgttt
    11221 ttatttctaa ctgcattaat gtttttacca tttgtaatgt tcatgatgac aagctttgtc
    11281 agaatagtta taagtttatc atttttgaaa tcagcattag gagcacagca agcaattcca
    11341 agtcaaattt tggtagggct tgcaatagta ttgacaatat ttataatgag accagtatta
    11401 aatgagataa acgaaaaggc attacaacca tatatgaaag aagaagttac tatggaagaa
    11461 gctatgaagg aagcagaagg acctattaaa gaatttttat taaagcaaac cagacagaca
    11521 gatttagatt tatttgtaga gcaagctggt ttaaaagaaa agaaattaac tcgtgaaaat
    11581 ataccattat ctgttgtagt tccagcattt gctataagtg aactaaaaac tgcttttcaa
    11641 ataggttttt taatatatat accatttttg ataatagacc ttgttgtggc aagtgttttg
    11701 atgtctatgg gtatgtttat gttacctcca gttatgatat cattaccatt taaattatta
    11761 ttatttgtaa tggtggatgg atggaacttg atagtaaaaa cattgatatt gggatttgga
    11821 taggagaaaa atagtatgag tgaatttatg ataatgagtg ttcttaaaaa tggcatgttt
    11881 acaggagcta tgattgcagc tccagtgcta attgtttctc ttgtagtggg gcttttaata
    11941 agtgtatttc aagccacaac tcaaatccaa gagcaaactt tgacttttgt accaaaacta
    12001 atagcaattc ttttagtagt agctattggt ggaagctgga tgttacatac tcttattagc
    12061 tttacttcgg agatattcaa tatgatgtct ctcataacta aataagtagg atatatatga
    12121 tttttgtatt tattagaata gtagcattct ttggaacatt aaggatacta tttccagctg
    12181 gtacacctgc tttatttaag tcgctctttg caatcattat atcaatcttg atttcaagta
    12241 ctatgcaaat tgaatatgca acaaatatag acaacattgt attgtttacc ttatatggta
    12301 taaatgagac cataactggg attatgcttg gatatataac caatctatgt ttttatagta
    12361 tcagaatggc tggttctatg attgaccaac agatgggatt atcaatgatt aatatgtttg
    12421 acccaaattc aatgactcag acaactttaa tcaataatct tatgaactgg acagcactta
    12481 tgatattttt tagcatggat ggtcatcatg ttttaattag aggcataagg tatagttttg
    12541 agttaattcc aatagggaaa tcattcatag ataataatat agattatata ataaacatat
    12601 ttgtgcagtg ctttttaact ggattcaaaa tagcaatacc tattgtactt tgtctgctta
    12661 tggcagattt tatattaggg cttatatcaa gaagtgtacc tcaactaaat gtaatgataa
    12721 ttggtatgcc attaaaaata ctggttggta ttgcattatt tatcatatca atacctttaa
    12781 ttgtcaatca aataagtcat ttattaagtc aaataccaaa gatgtatgag ggaacttttg
    12841 cactagctcc tatgtttttt atgggttcaa cagataaaac agaagaagct actccaaaga
    12901 aaaagggaga acaaagaaaa aaaggtaata tagcaaagag taaagaatta cccattgcaa
    12961 tgactctact tgcattcact ttattagtac ctacattatt ttcatatgta gtgaatacac
    13021 ttaaatcttc tcttaattat tttttgagtc tagattttta tatgaacata aattattcaa
    13081 gtatagagaa attagttata gcaggattaa tggatttttt taagatattt ttaccaatag
    13141 ctattccatt cttagtttta ggtatagtcg caaacctatt tcaagtagga atactgttta
    13201 ctggtgaaac tttaaaaccc aatttatcaa aattaaatcc aattagtgga ttcaaaaaca
    13261 tgttttctat gagaagtttg tcaaccttaa taaaagatac agcgattata tctattttgg
    13321 cttacatagg atatacattc ttccaagata attatttaga tatattaaag ttaggtaaca
    13381 tatacctgcc aactttaatg tacacagtta aagatttagt atattcaata ttaagtaaga
    13441 tttgtgtagc tatgatagtt atagcagttg cagattatgt ttatcaaaga tacagtcata
    13501 aaaagcaact tagaatgaca aaacaagaag ttaaagatga atataaaaac tcagaaggag
    13561 acccagaaat aaaagctaag ataaaacaaa aacaaagaca aatttcgtct caaagaacca
    13621 tgcaagcagt accaagtgct actgtaatag taaccaatcc cacccactta tctatagcag
    13681 tgagatatga aaaagggaaa gaccaagctc cagtagtggt ggcaaaaggt gcagattatt
    13741 tagcttttaa aataagggaa attgcaaagg gaaatgacat acctataata gaaaataaac
    13801 ctattgctag gttgttatat aagcaagtag aaatagacca agaaattcca gaggacatgt
    13861 atcaagcttt tgcagagata ttagtagcag tatataaaat caaaaataga tacaaagtcc
    13921 ccaaaaggta ggataagtaa tgaataagtt taatttaaag gaaaatatgg acgtaatagt
    13981 ggcatttggt gttgtaggga ttgttcttat gatgatagtg ccgttaccta catttatatt
    14041 agatatttta ctggcaatta acataagttt atctgtgcta attttactaa tgacactatt
    14101 tacaacaaat gtacttgatt tatctatatt tccaacagtg ttacttgtga caacactatt
    14161 tagattggga ttaaatcttt catcaactag attgatttta acacagggat atgcaggtga
    14221 agttatagag gcatttggaa gttttgttgt aggtggaaat tacgtagtag gaataattat
    14281 attcttaatt atattaattg ttcaatttgt tgttataacc aatggttctg gaagagtatc
    14341 tgaagtaaca gctagattta cgttagatgc catgccaggt aagcagatga gtatagatgc
    14401 agatttaaat gcaggagtta tagatgacaa aactgctaga aaaagaagaa aagagcttca
    14461 gcaagaagca gatttttatg gctctatgga tggagcaaat aagtttgtaa agggagatgc
    14521 catagcaggt cttatagtaa cagcaataaa cataataggt ggaattgtaa taggagctgt
    14581 aatgctgaat atgactctta tgggtgcagc acagacatat gtaagactta ctatagggga
    14641 tggtcttgta agccagatac ctgctctatt aatatctaca tcagcaggta taatcgtaac
    14701 aagagcttct actgatgaaa actttggtgg attagtaggt aaacagttaa cagcaatacc
    14761 aaaagcagta atgatgactg gctgtgttct aatcgtactt ggtatgatgc ctggattacc
    14821 taaaccagca tttttttcac taggtatagg tgctatagca gcaggttact ttttaggaag
    14881 agaaaagaaa gatactttag aagacttgga gtttgaaaaa cctgatatgg taaatccaga
    14941 taaaaatgaa gtagaagatg taacaaactt aataaatgta gaatcaatag aagtagaaat
    15001 tggatatggt ttaatatctt tagctgatga atcagcagga ggagatttac ttcagagaat
    15061 agcatcaata agaagacaat gtgcaataga catgggtata gtcgtacaac ctataagaat
    15121 aagagataat ttacagttaa atccaaacca atacaatata aaaataaaag ggaatgtagt
    15181 tggtgggtat gagataatgc ctaatatggt tttatgtatg gatcctatga atcaaggatt
    15241 tagtatattt ggaataaaga caattgagcc tacttttgga atagaggcac tttggataga
    15301 aagtgacaag gtagaagatg cagagctaaa gggttataca gttgtagatt catccacaat
    15361 acttataact cacttactag atataataaa atcaaatgca cacgaattac tgggaagaca
    15421 agaagttaag actattatag atgcagcaag agaaaattat agtgctgtag tagatgaatt
    15481 gataccagat ttaatgacac ttggtgaaat tcaaaaagta cttaaaaatc tgttaagaga
    15541 aaaagtaaat ataaaggata gagttacaat acttgagaca cttgcagacc agtctagaaa
    15601 tacaaaagat atagagcttc ttactgaata tgtaagaata gctttggcta gaagtatttg
    15661 taataattta gttgatgaag ataaggcaat tgtagtggct acattgtctc cagaaactga
    15721 agaattggta tctaataatt tacaaaggtc tgtaaatgga acttatccag cagttgaccc
    15781 agaaaataca aataaaatat ttgagagtat acaagaagta atgaacaatg tatatttcaa
    15841 tcataatata ccagtaatag tagtatcacc aaagataaga gctccattta gaaaattagt
    15901 tgaaattgta tatcctaatt tgactgtatt atcattaaat gaaataccta acgatattaa
    15961 aatcaaagca gaaggtgtgg tgagcatata tgatgacgaa aaagtataca gctaacacaa
    16021 ttcaagatgc tatgaatcta gccaaaatgg aattgggtga caatattaca ttaatagata
    16081 aaaaagaggt aagaaaatct ggaataagag gcattttttc aaagaaagat attgaattga
    16141 ctattggatg ggaaaagaaa gataatttag aacaaaaaga tttaaaacga gagatagaac
    16201 aactaaagtc aattataaat aatatggggt ttgataataa aaatgataat gatatagata
    16261 aaatatgcaa gaatttacta aacttagatt taaatcaaga aattgtagaa tttataaaag
    16321 ctgatttaca agaaatgaag ttcaatggaa ttgatacaag taaaaattta gtagaaagtc
    16381 taaagaaaaa gattaaaata gaaaatcaag caataaatgg taaaattgct ttagttggac
    16441 caccaggagt aggtaaaacg actacaatag caaagttggc tgccaaatta gtttttgagg
    16501 aaaataaaaa agtaggagta ataactatag atacatacag gattggtgct gtagaacagt
    16561 taaagatata tactgatata atgaatattc cttttaaagg ggtaataagc cctgatgaga
    16621 tggaattggc acttgatgag atgaaagatt gtgatgttgt attaatagat acaacaggta
    16681 gaggctataa aaactctatg caaatattag aaatcaaaaa tcttatagat aaagctgaaa
    16741 ctgataatat acatttagtt ttgaactgta caacaagaga aagtgataca aaagcaatta
    16801 tagattcata tagaaatgtg aattttaaaa gcttaataat tacaaagtta gatgaaacaa
    16861 taacatatgg ctcaatattt aacataatga attatgcaca aaaaccaata tcatatatta
    16921 caacaggaca aaatgttcct gatgatatta taaaacctaa tgaagataag attataagat
    16981 tactattagg agttgaaagt atatgataga ccaagctcaa attttgagaa aaatggcaat
    17041 ggaaaaaaga ggttctgatg agattatagt agagaatgag aatatgccta agataattac
    17101 aatagcttct ggtaaaggcg gcgttggaaa gagtaatctc gctacaaatt tgtctatatg
    17161 cttaaccaaa cttgataaaa aagttcttat attagatgca gatataggaa tgtctaatat
    17221 agatataata atgggggtaa atgtaaaagg gactataatt gatgtaatta atggcgaaaa
    17281 aaatatagaa gatataattt cacagactaa gtatggtgta aatataatat ctggaggttc
    17341 tgcccttaat catatagaag actttacaga agctcaaaga aataagttta tacatagcat
    17401 agaacagatt cacaatgtgg attttataat aattgataca ggagctggta tgagcaaaag
    17461 cctattgtca tttatatact gtagtactga atttttctta ataacaactc cagaaccgac
    17521 atcattgact gacgcatata gtttattaaa agctataagt aattttggca taaagaaaag
    17581 tgcaaatata ataatcaata gggtaattga tttagaagaa gcgaaatcta catacaaaag
    17641 aataaaaatg gttgtggaca aatttctaaa ccttaatctg gaattgtatg gatatataat
    17701 agatgacaaa aaagtaggtg tatgtgtaaa aaaacagata ccattcattt tagaatatcc
    17761 tacaagtatt gcatcaaaat gtattatagc tatagcaaag agaatggtaa gtcagtcaag
    17821 agaagatatt gttgataata aaagttttct aagaaagatg tttagtatat ttaaaagcta
    17881 aggaggcgta gttaatgaat agagaagaat taataaaaga gaatatgcct cttgtaaaga
    17941 gtatagcaag aaaatttttt ataccaggga aaggttttga atatcaagac ttagtaaata
    18001 gtggagtcat tggattgatt gatgcaataa ataaatttga tactgaaaaa ggagcaaagt
    18061 tttctagtta ctcttatata aagataaaat ctgctatatt agatgaaatt agaaatcaaa
    18121 gtccaatttc taaacataat ctaactaaag taaataagta taatagagtg gtagaaaagt
    18181 tgcaatctaa attattaaga gagccaactt catatgaaat tgcaaaagaa ttaaaggttt
    18241 cagaaaaaga acttcatgat atcgaaagta atatagatat gttaaatatt gtatcattaa
    18301 actatgtaat ctttgaagat acaaatgaaa ctgtacaaga tgtaataagt gatagagaag
    18361 aggaagctcc agaaaatata atagaagaag aagaaaagtt agagattcta tccaaagcaa
    18421 ttagtaattt aaatgaaaga gaaaaattaa tactttcact gtactattat gaagatttaa
    18481 atctaaagga gatagggaaa gtgctaggtg tttcagaatc aagagtttca caactccaca
    18541 gaaaatcaat aagaaactta agaaacaaga taaaagaact taaatattct atatagatgg
    18601 tgattgataa gatgatacaa attatattaa tagtagtgtc tattttaata atagtaacta
    18661 tagttataaa tttacagaag gaatcaaaaa ataaagataa cagatacttt gataaaatat
    18721 ttgagcaaga atatgaagca aatgatagat taataattgc agaaaaaaga agattctttt
    18781 tagacaaagt acttgcagaa aaaagaatta atagtcatat aaaagtttat gaaaattata
    18841 acctaataaa ggctaatgaa gaaagaaagg aaaacatatt tatagaatca aaaaaggaag
    18901 taagacaaga aaatgagtta tctcttaaag tgaaacatct tataagttca ggttatgatg
    18961 atgtggaaat atgtaagatg ctagatattg gaaaggggga actgttatta ataaggagtt
    19021 gttacaaaat ataaggatat tattatgtga taaaaaagta ttcttaggaa taggtattgg
    19081 tatattaata tcaactttat gtataatgct ttttagtaat aatgatatga gtaaggcaga
    19141 gatagaaaaa aaggccagag cgttaggcat gaaatataca agtgagatga aagttttaga
    19201 atagatgata ctgtttagac atttacaaaa aaggaggtca taatgtgata agaggattat
    19261 atacagcagt ttctgctatg ataactaatc aacaaaagca aaatgttgtt gtcaataacc
    19321 tagctaatat ggatacaaat ggatataaaa gtaaacaact tttgactaaa agttttgatg
    19381 aattaaagtt ggagggttac aataactacg caaatggaac taaacaaaag cagattatag
    19441 gcgatataag tccaggtatt tctatggatg aaactatcac aaattttaat caaggaccta
    19501 taaaaactac tgataataaa atggatgtag ctattcaagg taagggattt tttctagtta
    19561 gtgatgcagc tggaaatcaa ttttacacta gaaatgggaa cttcagaaca gacaatcaag
    19621 gtgaccttat aacaagtgaa gggtattatg tacttgggac gaatactgct actggtgctg
    19681 taggtcatat aaatgtagga aatcaaaaat ttgaagtggc aaaagataat acaatatcac
    19741 tagacggaaa tgctatgtat aaatttaata ttgtagattt taataactac aataacttaa
    19801 aaaaagaagg agataactta tattctggag gaggagctat gaaagtaaat aatgcactta
    19861 caatacaaag tgcgatagag tcctctaatg tagatatggt atctgaaatt aataatatga
    19921 tgacaatatc aagagaatat gaggcaaatc aaaagataat tcaggctatg gattctaaat
    19981 tagcaaaaat agcaagtgag attggttcag taagataggt aataggaggt gaatttttat
    20041 gtacacaatg ttttatacta gtaaaacagc tttatcagca aatcaaagta aactagatat
    20101 aatatctaac aatatagcaa attctgattc aacaggatat aaaaaaataa atatggaata
    20161 ttctgattta gtggatgaag tactaaatag accatcatac cctacaaatg gtaaagatat
    20221 atcgactgga acaggagcta aggcaacagc tcccatgaga aattatgaac aaggtgcatt
    20281 ggctcctaca gatagtaaga gtaatttggc gatagatgga gaaggattct ttagagtgat
    20341 aagaaaagat ggtacatatg catatacaag aaatggtgga tttaatgtag atgctttagg
    20401 taagattgta gatgataatg gaaatatatt agatgtccaa tttgatgcag gatataacta
    20461 taataataca aatatagata gtacaaacct gacagtttca agaaatggag agctattcgt
    20521 agataataaa aaaataggaa aaattaactt atatcaacct ataggaactc aaaattttat
    20581 atctgaagga gatagtttat ttgtcgcaac tgatgcagca cagattaaac aagttgagaa
    20641 agtcgatata gttcaaggct atagagagag gtcaaatgtt tctttacaag aagaatttgt
    20701 tgacttaata gccactcaaa gagcatttca aatgaactca aagggaataa agacagcaga
    20761 tgaaatgtgg caaatagcga ataatttaag agctaaataa ttatgtaagg agaggaatat
    20821 tcatgaatga tatgaaagag tacgattttg gcaaacctca gagttattca agagaaaatt
    20881 taacttgttt aaatttttta ttagctgaat tttgtaaaaa atataaaaac tatataattt
    20941 atgaacttaa gtgtaattct agtatggttg ttgatagtat agaccaagta aattatcaga
    21001 cttttttaga tagagtaact tcttcatgcg tagtagtaca aaatgcaatg gaacctgtta
    21061 tgaaaaactt ttcttttaga atagatagaa gtgtagctga tatgtggatt gatataactt
    21121 ctggaggaac tggagttgta aaggataatg atagagagct tacagaactt gataaaaaag
    21181 tgcttttaca ccttatggat gatttagtaa gaaatatgca cttatttgag ggttttgaaa
    21241 atatacaagt attggataca catacacata ttaatttgcc acaattatgt tctccaactg
    21301 caccagtatg cgttgtagat ttaaaagtgc taaatgataa tgagtgtatt ggaagtgttt
    21361 ctctttgttt tccatacaat ggtttagagg ctctgttaga aagtatatct gttatagaat
    21421 tttttgataa tgatgtagga gatgattcag aggaatttac ccaaaaaatc tataatagtg
    21481 tatctaatat agaattatca gttattgcag agataggaaa agtatcaatt aatgttgagg
    21541 atttacttaa actagaagtt ggagatgtaa tagtaacaaa taaaaaaata aatgattata
    21601 tagatatatt tgtagaaaat tcaaaatcat acacagcaac accaggattt atatcaagta
    21661 aaaaaggtgt aaaaataaat gatgctgttg gaaaagaggt gtagttatgg acaatagtaa
    21721 tcttatttca gatgaagaag caaagatgtt attaaatgat aaagttgaaa gtgaagttga
    21781 tggagatgat gagttagcct tagatgtctc tgaagttaat cgaaataata tacaaaggat
    21841 acttgacctt aagttagaat tatctgtagt tataggaaga actaagatga ctctaagaga
    21901 tattttaaat ttacataaag gttctcttat tgaactggat actttagcag accaagatgt
    21961 ggaaatacta atagataata aggttttggc ttatggaaaa gtagtggtgg tagatttaaa
    22021 ttttggtgta aagataacaa gtatagtaag tgaagaagac atggtaaaat ctcttgttta
    22081 gagataacat caattaatac ttaaggcatg aggtggtact tattttaaac ttaaatgata
    22141 taaaaaaaga tatactaaaa ctcaataaaa aagactcatg ttctctatat aatgaatcta
    22201 ttaataatat aaataataat tgtatatcaa ctggattaaa atttttaaaa caagcaagag
    22261 atttaaaccc agatgatgtt gatatattaa atctcacagg tcttgtaaac ttactaaaat
    22321 gtaattttga tgatgctgta gaaagtttct ataagagttc actttgtgaa aaaactgctc
    22381 tgtgtgaaaa gtatataaac atgcttacat ctgaggagtt tttaattttc tttgaaaagt
    22441 acaatcaact tataaggtta ataaataaag gaatgtataa agaagctata caagggttta
    22501 aactcataac tgaccaatac tgtgatttaa tagaacctta tgagttgctt gccttactgt
    22561 atgacaagga agaagaatat ataaaatttg atgaatgttt agaagtattg aagattattg
    22621 ataaggaaaa ttatttatta aatcaagaaa ataacacatt agatatgtat aatagtgcta
    22681 atatacaaac taaaagtaat ataaaagtag atgattataa gaatgagttc ttacataaaa
    22741 gtagtagtaa ccataaaatt aatgattata atgatgtaac taaagaaaag agttcaagca
    22801 aaaaccagaa aagttcagat tctaataaga aaaaaagtat taaaaaaaat aagtacctat
    22861 atatagccat aatattagga atattattag taggacaaag tatatataat tcttataaga
    22921 tgaataattt aactgataaa atatctaaaa ataatgaaac taaaaatgaa gagtcaatag
    22981 attctaactt gactaagaaa gaaaataata tagaagaaaa tgaagatatg agtaaggctt
    23041 ctaatataaa taagagtaat aaaaacatta ataaagagga tgatgtaaaa tctaaaaaaa
    23101 atactgtaga aaatgaagat gataaagatt ctgacttatt tacagaggat gaattgatgt
    23161 ctaaggcgaa tactttaaaa tcagaaaaga aaactaaatc ttctatacaa ctatataaga
    23221 aagttgcaga tataggtgaa aataaaggga atacatctga agcaacttat caagttgcta
    23281 ttctcagtga aaagttaaaa gactataaaa ctgctgaaga atattataaa atgtatgtag
    23341 aaaattactc agaaaaagat gcttattttg atgaatctta ttataattta ggtatgatgt
    23401 actataacaa tggtgattta aaaaattcta aattgacat
//
LOCUS       DAGSHT010000039        23252 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGSHT010000039 DAGSHT010000000
VERSION     DAGSHT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.040"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(283..441)
                     /locus_tag="MJB72_001407"
     CDS             complement(283..441)
                     /locus_tag="MJB72_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262528.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(735..2105)
                     /locus_tag="MJB72_001408"
     CDS             complement(735..2105)
                     /locus_tag="MJB72_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBY8262529.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            2321..3438
                     /locus_tag="MJB72_001409"
                     /pseudo
     CDS             2321..3438
                     /locus_tag="MJB72_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3801..3881)
                     /locus_tag="MJB72_001410"
                     /pseudo
     CDS             complement(3801..3881)
                     /locus_tag="MJB72_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(3921..4589)
                     /locus_tag="MJB72_001411"
     CDS             complement(3921..4589)
                     /locus_tag="MJB72_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY8262530.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(5442..7862)
                     /locus_tag="MJB72_001412"
     CDS             complement(5442..7862)
                     /locus_tag="MJB72_001412"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBY8262531.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(8216..8566)
                     /gene="rsfS"
                     /locus_tag="MJB72_001413"
     CDS             complement(8216..8566)
                     /gene="rsfS"
                     /locus_tag="MJB72_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBY8262532.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(8683..9255)
                     /gene="yqeK"
                     /locus_tag="MJB72_001414"
     CDS             complement(8683..9255)
                     /gene="yqeK"
                     /locus_tag="MJB72_001414"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBY8262533.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(9256..9945)
                     /locus_tag="MJB72_001415"
     CDS             complement(9256..9945)
                     /locus_tag="MJB72_001415"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBY8262534.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            10221..10859
                     /gene="recX"
                     /locus_tag="MJB72_001416"
     CDS             10221..10859
                     /gene="recX"
                     /locus_tag="MJB72_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBY8262535.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            11012..13015
                     /locus_tag="MJB72_001417"
     CDS             11012..13015
                     /locus_tag="MJB72_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBY8262536.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            13189..13830
                     /locus_tag="MJB72_001418"
     CDS             13189..13830
                     /locus_tag="MJB72_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8262537.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            13923..14606
                     /locus_tag="MJB72_001419"
     CDS             13923..14606
                     /locus_tag="MJB72_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262538.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(14783..15094)
                     /locus_tag="MJB72_001420"
     CDS             complement(14783..15094)
                     /locus_tag="MJB72_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBY8262539.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(15172..15990)
                     /locus_tag="MJB72_001421"
     CDS             complement(15172..15990)
                     /locus_tag="MJB72_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8262540.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            complement(16090..16614)
                     /locus_tag="MJB72_001422"
     CDS             complement(16090..16614)
                     /locus_tag="MJB72_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY8262541.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIDRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            complement(16633..17775)
                     /locus_tag="MJB72_001423"
     CDS             complement(16633..17775)
                     /locus_tag="MJB72_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY8262542.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            complement(18055..20442)
                     /locus_tag="MJB72_001424"
     CDS             complement(18055..20442)
                     /locus_tag="MJB72_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8262543.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            complement(20435..21118)
                     /locus_tag="MJB72_001425"
     CDS             complement(20435..21118)
                     /locus_tag="MJB72_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262544.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            complement(21235..22545)
                     /locus_tag="MJB72_001426"
     CDS             complement(21235..22545)
                     /locus_tag="MJB72_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8262545.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            complement(22539..23246)
                     /locus_tag="MJB72_001427"
     CDS             complement(22539..23246)
                     /locus_tag="MJB72_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8262546.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
BASE COUNT         7846 a         3843 c         2731 g         8832 t
ORIGIN      
        1 aaaataaatt gagtatttcc aatgcctaca catatacaaa tctctcaaat ttgcagtgaa
       61 ccatgagtag tgcaattgat aacacttatc acaagtcctc aatgccttat atttcaactg
      121 ttaagcctta atttcttcaa aaaatcgaac actgcaaaat ttctatgact ttattatatc
      181 ataaaaatat tatttttgaa tatctgtacc aatttgtgat ataataaaag caagaagaac
      241 tacaatctat tttgctgtag agtggagttc ataatttaga aattacttag ttttatggaa
      301 tctgattttt aaatcaaatt cccaaccact cttagttgcc gctttgagtg gttttttacg
      361 ctttctaaat agtttgctag ctatataaac tattaagcta gcagatagac ttgctagtat
      421 gccttgtaaa aaattatcca tgtatattca cctcccttct taacgtcggg aggataattt
      481 tttgatacat gaactccact ctataaattg tagattacat cttcttgcta aaaatattat
      541 aacatataat tcttacatat tttacctatt tattactttt tacattataa taatattctt
      601 ttaatcaaaa aaacacctac tattaaagta aatgttttta agtattttta attttaaaat
      661 ccatatagtt catatgaaac taaatataat aactcaacat atgagcaata tcgagacatt
      721 tacataccca ctagttattt atattcaaaa aaatcttctc catccgggtt ataaaataat
      781 attctacttg ccaatccttg ttcatattta taattttctt tatcacctac ataagaaaat
      841 gttaatgtca tgcttcttga tggatctaat gaaattatat ctaaataaat agtacatttt
      901 tctcctggct ttaatacacc gaaactatta aaatccattt ttattttagc gtattgcccc
      961 atatataatg ttgaattagc ttctccataa acttttaatt ttgttacatc taaattttgt
     1021 gtacttgtat tttctatatt tataggaact gttgggtctt tatatttttc agtataatat
     1081 tcaaggttat catcataaca ttcaagataa cttcctttat cttgccaaat tatgtgattt
     1141 gaatcaaaat caatctttaa tccacatatt atatttttat ttgaagatgg aagactaata
     1201 ttattgatgg tattttcaca ctctagcata attttatcac ttagatttcc tatcccggtt
     1261 acttttgcag catttttaag tatatcttta ttacttccaa tatcaactag tacaatagga
     1321 ttatttttag ccattggtga agcaactaaa gcataaacta aatcagtacc actagctata
     1381 taaaattctt ttgcgccatt atagaactta tttactattt ttttattagt atcatatcta
     1441 tctactccac ccaatcgagt tgaattagta tcatttacta aactatcact cattgatgaa
     1501 gtgccaccta ttgcataact ttctatacca gttgtattaa atggaacact ttttccatca
     1561 gttaatacta tagctgcttt atctctaact gctacaggtg cagcactcat agcatctggc
     1621 tcacctttga aagcgttagt taatattact ttatttactt tattaatgga atttatttct
     1681 tttgctacat tataacttgt cttaatccta tcattacctt gaagccttct agtctctatt
     1741 cctttatctt ttagaaaagt ttctgttgct ttatcaatgg aactttctcc tcctattatg
     1801 tatacctttt tagctttttc tactctttta agagttgcat taggtatatt attcttttta
     1861 gtcaaaagga ttggtgcatt tgttgctcct gcaagtccac tcgcacttag cccatcagcc
     1921 attgtactat ctgcatttat aagtattgca gtagtataac tttgtttgtc agctatgata
     1981 ccagcagttt cgtatttatc tgctccttga attttatcaa ttttatcaag tgcattagct
     2041 gatataggaa aaccagcaac aaacattgac actaacccta gtgataataa tttctttaat
     2101 ttcacaatat ctcccccttt tatatatgaa atactatata tcttaataat tctacattaa
     2161 attttctttt cctcctttaa cgatatttat cttcctgtta atttaagtgt atacaaatac
     2221 gtgttataat gtaactgtaa agaattaaaa agataatgac ataattagag tagtacaact
     2281 gttcttttat aaaactaact tataaaggga gcgtacatat atgaatatca aatcagcttt
     2341 tataagaaaa agaggggaaa aattctatgt atatgtggag tacatagagg aaacaactgg
     2401 caagataaaa cagaaaagtt atggaagtta tgaaaagaaa aaggatgctg aaaaacattt
     2461 aattgaaata aaatctacta taaacaataa taagtttgtt gctccaaaag atatgacatt
     2521 tgtagaaaga tgttataaat acatggacat gaataaaaat aattggtctc catatactgt
     2581 tacaaataca aaatcttggg ttaaaaatta catagaacct ctttttaaga atctaaaact
     2641 cacagaagta aatcctagtt cagtacaagc ttttgtaaat tataacttta ataattctac
     2701 ctttactagt gctaaagtaa ggtatagctt tctaagttct gtaataaggg aagcatatag
     2761 attaagagaa atacaagaaa attcatgtga ttttataaaa cttccttcta aagaaaaata
     2821 ttctacaaat gaaatataca atagaaaaga agtattattg ctaatagaaa agttacttag
     2881 taataatata gaaataccta ttcttttaat gttacttcta ggtttaagaa tgggagaagc
     2941 tactgggctt aggtggtcag atgttgactt agataacaat ctgataaatg ttaatcaaat
     3001 tcttgtctat tctaatggca aaatatcttt taaagagcca aaaacaccaa aatcaaaaag
     3061 agctttatca gctccaaaag aattaattga aaaattgaaa attaaaaaac taagacaaag
     3121 caaattaaaa ttgtaaggta tcctggaaaa cagaaataat ttagtttgtc taaacaccaa
     3181 tttaaatccg tttctacctt cttcactaag aaaaacgttt cacaatttta caaaaaaaat
     3241 aatttaaaaa agattagaat acatgattta agatatacaa gtgccactct gctgctttta
     3301 gggggaacta atatgaaagt tgtttctgaa agattgggtc atacagatat aaaaataact
     3361 atgaatagat attctcatgt acttaaagaa atggacaaag aagcttccga caatctaagc
     3421 gaacttctat ttaaatagta aaaactgact gtcagttaaa tgtcagttaa acctaaaaaa
     3481 ataggttttg tcagttgatt atcagtcaat tattttataa tagtttcact taaaaacgtc
     3541 tatatctaca cattttacta tttgtttatg cttattaata tctatctgtt attattaatt
     3601 ctaatagctt ctatttaatt atttagttca gtttacagga aactacacac aaaccataaa
     3661 ataggatgtt gacaatataa cagatagtat gctcctgtaa aaggtaacgt actatctgtt
     3721 atgttgtctg gatttccaaa tggttaccct ttggcacaca actttgctat ctccttggta
     3781 aactaaaagt ttgtaatcta aaatcctaat ccatctctat aaaatttaat tgacttctcc
     3841 atacttttta cacctaaaca tataaaagta attttattca cttaatgacc tccataattc
     3901 ccaaattagc tatttggaac ttaactaaaa aatcctaata taagggcaac tattataatg
     3961 gcaatcggaa tacatactgt gtaatcctgt tccatagagt catcatctga atgtagtttt
     4021 acaattcgca atttagcaat cagtttgaag cagaaatagc ctattattgt tccaattaca
     4081 ttcgtgagta agtcgtctat atcggttgct ctgtgtaacg taaaaagctg aatagtttcg
     4141 ataaaaagag ataatccaaa accaatcaag aatgtatttc tcaaacgttc aaaggttttg
     4201 ctgataagcg gacataaaaa acctaatggg acaaaaagta aaatattcaa aataaaactc
     4261 aaactaaatc catcactaaa aggaataaga tttatgtttg gattaaagaa cgtttcccct
     4321 aactgtgaca gtcttatata ttcactgaat gtagggatac caacaatatg cgatagtgtt
     4381 aaacataagt agtaatacat tgccagtgat aaaatagatg tttttacctt tggaatatca
     4441 ctatttttct tgaatattac aactaagaaa ataccaatca agataattcc caacaagaaa
     4501 ataggattaa ttaacaataa atctttgata gcttcaaaca gactgagttg aatttctaca
     4561 cctgattgat tatttgttac agtctccatt tgtacatacc tcctcataac atttcaatta
     4621 tttctaaaac acaaaataga agataaatct taaaatcatc ctatcaaaat ttttaaattt
     4681 gcctttatca gtttatacca ccagttaatt cctgtcttac ttctgtatgg tattcttggg
     4741 attctttcat caaattagac aaaaaatggt taccatatca ataaactggt tacgctccat
     4801 atctcccgaa gcaaccatcc cctgagaata aatccgtatc aggaatctcc acatctactg
     4861 cacctatcac aagttatttg ataacatcct cagacaatcc caacttggat atatcgacaa
     4921 cacccattag aataccactg ctgaccatac ttgttttcct gctgataatt ttaatcaact
     4981 gggaagttga aacgctatct tttttacaag ttccttttta gaaacatggt agctattata
     5041 tgaatctgaa tttcgttgtc tgggtgtttc atataaagca tagatacgca ccttttttgc
     5101 ctgttttcat tatatagaaa aaaatttcaa aatacaataa ttatattttt ttcgtttaag
     5161 cttagagcag ttccttgatg aacaacactt tggaaactga atgatttaca taccacttaa
     5221 tacattaact cgatttaaca ttaattataa taatatttaa aacaacaact agggtatata
     5281 agaatgaact ttttagttct ttaatcattt ttttgtataa aatcaaatct atttttataa
     5341 tttcaacaat tttataattt caacaatgaa aaaaggctta tctctatttt gagacagcct
     5401 cgtttatttt ataaatttat atttttgttc atatttttga gttatttaac aactatattt
     5461 actagttttt taggtacagc tacaacttta actatagttt taccttcaac taatcctttt
     5521 attttttcat cttctaaagc taccttttcc atctcatctt tagatatatt tgagttaaca
     5581 gttaattttc ctctaacttt tccattaact tgtacaacaa cttctatttc atcttttact
     5641 aaagcttttt cgtcatattt aggccagtct atgtcaaata cacttccttc tttacctatc
     5701 atagtccata gttcttcacc taaatgaggg gcaaatggtg caattatagt tactatagtt
     5761 tgtacacctt cttttataac tgcttcgttt atattatcta actctttata tttatacata
     5821 tcatttatta attccataag agctgatata gcagtattaa atccgaattt ttcacttaaa
     5881 tcagcagtaa cttttttaag tgtagagtgt atagtatatc tcatatcctt atcttgggaa
     5941 tttaattctc caaactctac atccttttta accacatctg ctaactcgtc aaccaatcta
     6001 taaaccctat ttaagaatct aaagcatcca tcaacacctt gctctgacca gtccaaatct
     6061 ctttctggtg gtgctgcaaa taatacgaaa agtcttgcag tatctgctcc atactcatct
     6121 attatatcca taggagatac tgtattccct ttagacttac tcattttaga accatccata
     6181 agtaccattc cttgagtaag taagttttta aatggctcat tgaagtctac cattcccata
     6241 ctcttaaatg cctttacaaa ccatcttgca taaagtaaat gcattatagc atgttctact
     6301 ccacctatat attggtctac tggatgccat ctattaacta attccttact aaatggttca
     6361 ttttcattat tgctatccac atatcttaag aaataccaag aagaatcaac gaatgtatcc
     6421 atagtatcaa cttcccttct agctggtttt ccacagtgag gacaagttgt tgacataaat
     6481 tgctttgaag tcgtaagtgg agattcccct tttccagtaa attcaacatc tgttggtaaa
     6541 agaactggta aatcttcctt tttaactgga actattccac attcatcaca ataaaccaca
     6601 ggtataggac aaccccagta tctttgtctt gaaactaacc agtctctaag tctataatta
     6661 attgttctct ttcctctacc ttctttttct aacttatcga ttattccttc aaatccttct
     6721 gaagcatcta atccattaaa ttcttctgaa tttatcattt tgttatcttc tgttattaca
     6781 tcaattatgt ctaaattata tttttcagca aaatctctat ctctttcatc atgtgctgga
     6841 actgccatta tagcacctgt tccatactca actaatacat agttagctat ccatagagga
     6901 actttcttgc catttaaagg atttataaca tatctaccta taaacattcc ttctttttct
     6961 acatcagctg ctgttctttc tatttctgtc atagtatgca ttttttgaac aaaagcttca
     7021 acatctgctt catattcagt ccctgcaacc aattctttaa ctaattcatg ctctggagca
     7081 agtaccatat aagtaactcc ataagttgtg tctggtctag ttgtaaatac tgtcatagat
     7141 ttatcagttc catctatatc aaaaactaaa tctgctcctg tacttttacc tatccaattt
     7201 ttttgcattg ttttaacttt ttctggccat ccatctaata tatctaagtc attaagtaat
     7261 tcttctgcaa attcagtagt cttaaaatac cattgttcta aatctttctt taatactgtt
     7321 gctttacatc tctcacatgc tccttgaaca acttgctcat ttgcaagaac agtctcacaa
     7381 gaaggacacc agttaacata tgattttttc ttatatgcaa gcccatgctc taaaaacttc
     7441 aaaaatattt cttgtgtaaa tctatagtat tctggcgttg aagttgcaac ttctttatcc
     7501 caatcataac taatacctaa taaatttaat tgctctttca tttcttctat attttccatt
     7561 gtccatttat gtggatgtat tccatgtttt atagctgcat tttcagctgg taatccaaaa
     7621 gaatcccatc ccattggatg aagtacatta tatccttcca tttttttgaa tcttgcgaca
     7681 acatcaccta tagaataatt tctaacatgt cccatgtgta tttttccaga tggataaggg
     7741 aacatttcta atgtgtaata attaggtttt tcagtttgtg cagtatccat tttatactga
     7801 ttattatctt tccagatttt ttgccatttc gattctactt ctttaaaatt atatacgctc
     7861 atattttgcc tcctattttt aattgtgtat aactacctta tttagtatta tttacttata
     7921 atttccaaat aattctttga aaataaaaaa tcctcgtcac taaacttacg tttagggacg
     7981 aggataaatc ttcgcggtac caccctaatt agcctactac atatatagac tctcttagat
     8041 agcttttaat ccagccatgg agaatagatt ttaatctagt aagttcaaag ttatataata
     8101 ttagttctca ccttccacta actctctgaa aaaatataat cttttactgc tctagtctat
     8161 tcaatttaat ataatttttg aaattttcta attataataa catatattat catgtttatg
     8221 ctaatgtgtc aatatctata taaggtgcat ctttccataa tttttctaaa ttataaaacc
     8281 ctctattttc ctcattaaat acatgaacca ttatgtctcc ataatcaagg agtacccaag
     8341 tttgagttcc ttgtccttct ttacctcttg gctctaatcc tagttttgct aattcatctt
     8401 ccacattgtc agcaatagct tttacctgtc tctgagaaga tgctgtagta ataataaaat
     8461 aatcacacaa actagaaact tttcctatat ttattataac tgtatcttga cccagtttat
     8521 cttctatggc atcatatgct atttttgtca tttgttccac tgtcatataa aaattcctca
     8581 ctttcctaat taaaatacac ttagttattg taacatatta atacgatttt gtaacatagt
     8641 caaatttaaa tttattaata tgtttgcctt tatatagctt ttttataata acttttcttt
     8701 aattagatag tttcttgcca taactgttct tggatgtatc tcttcttttt tattgatgac
     8761 aaacattaaa gtattattaa aagaagttaa aagtgcttca tcaagcttcc ctccatatgc
     8821 taattctctt aatgtatcta caactgggaa ttttcttcct tcttcaatta aatcagctat
     8881 gtaaattatt ttttctaaaa gagacatgtc ctctctccct gttgtatgat atttgattgc
     8941 atttataata tcttcatctt ccacttcaaa ttcatactcg catatgtacg ccccaattac
     9001 actatgagac aaggaacgat ttccatcctc tattgggtct aaatatattt catacttatt
     9061 tacataatcc tctatttcct tgtcacttaa atactttgca caatcatgaa tcattcctgc
     9121 taagtaagct ttttctttgt cacatccata aatctcacat aatttttcag cacattttgc
     9181 aacatttttt gaatggctaa gcctccccac tggtagcatt tgatttagtc tttggttaat
     9241 attttctaag ttcatttaat catctccata ttgatatagt ttattttcat ataaataatt
     9301 ttctacattt tcgggtacta agtaacgtat tgacttgcct tcatttagct gttcccttat
     9361 atatgtggac gatatatcca gtgatggaac ataaactgtt ataatgtcag cattatactt
     9421 cttagttagt ttttctatag tctcttgtga ccttaacaaa ctaattcctg gtctagttgc
     9481 ggctataaaa gtagctaact caaagttttt ctttacatct ttccactctt caacactaca
     9541 aattgcatct gcacctgtta taaaataaat gtctgcattt ttgtattttt tctttaagta
     9601 ttttaatgta tctattgtat aagtcctttt ctttctttcg atttctactt ttgaaactaa
     9661 aaaatcttcg ttgctttcag ttgctagaag tgtcatatta tatctatcat acttatctgt
     9721 agtgatacat aatttatgtg gtggatttcc tgatggtata aagattattt tgtcaatgtc
     9781 atacttgtct ctaataaatt ctgctgttgc taaatgagca tagtgtatag ggtcaaaagt
     9841 acctcccaat atacctattt tgattttgcc tttatggcga ttaaatttct ctagtttttc
     9901 agtatttttc aactctgcca tcttaactag attttcactt ctcatatttt gtctcctttt
     9961 aatgttataa tactattgta ccattatttt ttatttctta aagcttataa tttgtttttt
    10021 tgggtatata attatattta tatgctaaaa aacactttaa aaaataatta tttaacatga
    10081 ttctgcttta ctttttaata aaaataactt tttactattt tgtcttaatt ttgataaaat
    10141 ataacataca aatataagtt ttttgttaaa ttttattagt tgcttttgtt aatataatta
    10201 catataaaaa aggaggactt atgggtataa ttacaaaaat agaacaacaa aaaagaaatg
    10261 atgatagagt taatatttat gtagatgata aattttttat tgctatattt aaagaactgg
    10321 tttatacatt taatttaaaa aaagggaatg aaataaatga agaagaatta aaacctattt
    10381 tagatgatga aatgtatata aaagctaaaa ataaagcatt aaatatttta tctcatgctg
    10441 accaatctga aaaaaaatta aaagaaaaac tatcatcaga atttgatgaa aatataattg
    10501 agagggtcat agattttctt aaaagttata atctcgtaaa tgacagtgta ttagctcaaa
    10561 aaattgttaa cacaaatgtt aacctaaata aatgtggcaa aaacagaatt aagcagaatt
    10621 tatataataa aggtatcaat agaagtacaa tcaatgaagc agtatctgaa ttagacaagg
    10681 acactgaatt tgaaaatgct atgtatctag caaaaaaaag gtatgaaaga gtaaagaaag
    10741 aagataaaaa gaaaatatat caaaaaattt ctcaacatct ttcctataaa ggctttgatt
    10801 atgatattat taaaagagtt ttaaacaaac ttttaaattt tgatgaatac gatatataag
    10861 tataaatact agggaggtaa attatgaaat taatcattgg tggcttaagc tttttattag
    10921 gattatataa tatatatcaa ttatacagag aagatagtat gagataaata ttatttttta
    10981 gatgtaatat ttattatgga ggagaaatac aatgaaaagt caaaaaagat ttaaaatttt
    11041 agatattgat gtatacctac aaccatttga atctgatata aaaaagcgaa tgaaacatta
    11101 tgaggatatg aaatcaaaaa cattgaaaaa ctataaagat tttagttctt ttgcaaatgc
    11161 tcatctttac tatggatttc atcagacaaa tgatggattc ttctataggg aatgggctcc
    11221 aaatgcagat agtttgtcac taattggtga ctttaataat tggaatagaa aatctcatcc
    11281 tctaagaaaa attgggtctg gtaattggga aatatttatt cctggtcaaa atagcttacc
    11341 tcacaaatca gaggtaaagg tacaagttac tgcaaatgga aaaacatttg accgtatacc
    11401 attatatata aaaagagtaa ttcaaaaaga ttttggtgga tttaatggac aaatatggca
    11461 accaaaaact ccatttatct ggactgacaa tgattttgac ttaaaaaaca taacttctcc
    11521 actaatctat gaatgtcata taggtatgtc tactgaaagc gaatctattg gaacttataa
    11581 tgaatttact gaaaaaatac ttcctaagat aaaaaaagct ggatacaata ccattcagct
    11641 tatggctatt atggaacatc catattatgc atcttttgga taccaagttt caaactttta
    11701 tgcaatctca tctaggtttg gtactcctga agacttaaaa aacctcataa acactgctca
    11761 ttcaatgggg attgctgttt tattagattt agttcattct catgctgtaa agaatacttt
    11821 agaaggaata aatgagtttg atggaagtga acatcaattt ttccactcag gttcaaaggg
    11881 aaatcatcct gcttggggta caaaactttt taactatggt aaacctgaag taatacattt
    11941 tttgctttca aatataaaat tttggttaaa tgagtaccat tttgatggct ttaggtttga
    12001 tggtgtaact tcaatgttat atcataacca tggtcttgga gtttcatttg atagttatga
    12061 aaaatatttt agtatgaata cagatattga agctataaca tatctccaat ttgcaaatga
    12121 attaatgaaa gaaattaaac ctaattcgat aagtattgca gaagatatga gtggaatgcc
    12181 tggtatgtgt atccctataa aagacggcgg aattggattt gactatagac tcgcaatggg
    12241 agtacctgat ttttggataa aaactatctc taacttatct gatgaagact gggatttggg
    12301 caaaatgtgg tatgaactta ctactagacg acctggagaa aaaaatatag gatattgtga
    12361 atcccatgac caagcattag ttggcgataa aacaattatt ttttggctag ctgacaagga
    12421 aatgtactgg aatatggaaa taaactcaaa taaccatgtt atcaatagag caatatctct
    12481 tattaaatta attaaattaa taactttttc tctagcaggt gaaggatatc taaattttat
    12541 gggaaatgaa tttggacatc ctgagtggat tgattttcca cgagaaggca acaattggag
    12601 ctataagtat gccagaagac aatggagcct ttcagaagat aacaacttaa agtataaaca
    12661 attattaaat tttgataaat ctatgctcga acttacaaaa tacagtgata tttttatcca
    12721 aaatagtcat gaacttatac atatggataa tgataaaaag attttggtgt acagtaaggg
    12781 caaatattta tttatattta atttccaccc taatctaata caatcagttg atatttcaaa
    12841 gtttcaaaat acaaactaca aaacgatatt aaatactgat atggtagagt ttggaggaaa
    12901 cacaaaaaaa gaaaacttaa gagattatga tgtatacttt agctcaactc ctccttcttc
    12961 taaaatgcct attgcctctc gtacagcaat agtgctttta gataatggta tttaaatatg
    13021 agccaaatat attaggaaac tgcaaatata gtccagtata aggcaaaaaa ataaaataat
    13081 gaaagcatgg caatatacct gttcaacatg ctaaaaagac acattgacaa atcattatgt
    13141 atttgctaag ataaacatac caaatgattg tttcgaggag tgagtttttt gggaagaaaa
    13201 aaagaacctg ataatactat acaaaaaata cttgatattt cacataagtt attcttagaa
    13261 aaagggtatg agcagacaac gattcaagac attgtagata atttagatgg cctttcaaga
    13321 ggtgctattt atcatcactt taaatcaaaa gacgaaataa ttaaggcaat cacaagtcgg
    13381 ttgtataatg atggatttta tgggtatgag gtaaaaaatg atgaaggaaa gaccgcatta
    13441 gaaaaattac aatataggtt gcttcatgct tatgaacagc acaataaaca agacattttt
    13501 ttaatgtcat catcactatt gcgtaatcct aagtatttag ctacgcagtt tgatgagatt
    13561 ataaatgata ttgtgccttg gataaaagca ttgattgaag aaggaaatca agataattct
    13621 atggaagtta aaaatccaga gattgctgct caaatgtttg gttttcttct aaacacatgg
    13681 tgtattccaa cagtttatcc aggtaatcca caacagatga tagaacgttt tactttgttt
    13741 ggtgagcaag ctgcttttat gggtatttct tttatgaatg ataagataat gtgtgaatat
    13801 caaaaatttg ttaataaatt ttctaaatag taaatataaa actgccttaa tatcggcagt
    13861 tttatattta cacaaaacaa acatttagtt ggtattttaa aaaatttaat ttatttcagt
    13921 acatgcaaaa ggaggttgct atgaacaaac taattaaaga ttgtgtaaac ttttttaatg
    13981 attgtggctt ttcatattca atatgtggtg gatatgccct agaattattt gcgaatatgc
    14041 atattcgccc acacagtgat attgacatat ggatttctga cacagataaa gaaaagagca
    14101 ttcagtatat gcttaatcaa ggatgggata tatatgagcc cttgggtaat tatttattac
    14161 gacctattat cgaccttaac aagcaacata tagagcaaat ttgtgttttt gcgattaagc
    14221 ctgattgctc atttattgaa ttaaaaaaag tcgaggatga taactataaa atgaatatac
    14281 agtgtgacga acagttacag ttcgatttta ttgaattgat atataatcca cagaaagatg
    14341 gaaaatttct tttttcccaa aatcaaagaa ttataagaga tatggataag gccagactga
    14401 taacatcaga taatatccca tatatggctc ccgaagtggt tttgttttgc aaatcaatat
    14461 acatagatag gcttggttat caaaaagact tcgatgtaac agtcccacta ttatcacaag
    14521 agcaaaggga ttggcttgta aatgctttaa taacagcata tccagatgga catgcatgga
    14581 ttgaccaatt ggcaaatcag tattaagcct tttagctccc gaatatgaca ttatgataat
    14641 ccacactcta acagtttgtt ggtttttcta gtatataaca ctcatattaa aataaacact
    14701 aaaagccaaa taacaattta aatacaaaat gttatttggc taatttagtt tgatactatt
    14761 taactatctt ttacattcag ttctataatt tccaactttt aatttgtggt attatattca
    14821 tcttaacagt ttgattcttt aaagcttttc taaatttctc agttgaagaa tctttaaaca
    14881 ttttactaga aacactcttg aggtcttcta tagattccca atgttgtatc ataatccact
    14941 tttgagactt ttcatcatat aaaagctcag tatcaataaa accagattga atagaatgaa
    15001 attttaattc taagtcattt acaatttcta taaactcttc ttttgataaa ttttcttcca
    15061 cattaaactc aactatttct gtaataattt tcatatgtaa aacctccaat ttattttcct
    15121 atatcatatc aaaaaataaa ttggaggtat tgtaaaaaat cgacagtata attataaata
    15181 gctgtacttt atattttctt taatcgtctt attctcacca ctaaatttaa ttggtgacgt
    15241 ccctgaaaat tttttaaatt cttttataaa gtgtgcttgg tcatagtact cattttcata
    15301 aataaaatca aaaagttcat catctttata attataaata agttgattgc ttgccatttt
    15361 aaatctgtaa attctcttta aaatcttagg tgtataccct gtatatttta aacagattct
    15421 ttccaatgtt ttaatattta tatttgtatc atcacaaaat gcttttatat tgctatatct
    15481 attaacatat ataaatgaat ttagaagctt tacatattta tttgaaatta aatcttcttc
    15541 taatatggat aataatactt cttctaaaca taaaacaact ttttctattg cttttgttgg
    15601 atttaatgac tcttctaatt tttttgcaaa tagttttgat actgcttcta ggtcaacaat
    15661 ttgtctgtta aactctgaaa ttggtatctt taaaaatgga tagagaccaa atggataaaa
    15721 agaaatccct ataacacgta tatttccatg ttgaataata aatccatgtt tatcccttat
    15781 tccattaaaa tgaatattac tagcaatata gtcttgattt tcaattttat attccataac
    15841 atcagaaaga ttgagaataa tatctatact gtctgtcgga agtaacttat tatttaacag
    15901 tatatttgat tttgtctcaa aataccaaat aaactttacc cacggcttta aacgaacatt
    15961 tttgattgaa tatcttgtaa ctgataacat tgtgaactaa cctctatact aaaatttttt
    16021 aattatgtac tccatcctaa tattatacag tataatttat gctatcataa attttagttc
    16081 actttatatt tattttatac attttttcaa taccttagaa ataggaagcg ctattataag
    16141 acctatcaat acttgcatta aattacctgg tattgaagta agaggtacta taaaattacc
    16201 ataaagtata acttctgtta cataatatcc aaatagcatc caaattgatg aaacacatat
    16261 tcctattaca ttgattaaaa aattattccc attattacca tttgcccatg caatctttcc
    16321 aagtaaataa cccataactc ctctatctat aaaggtaaac ggtgcccata aagtccaccc
    16381 agatatcaaa tcaaaaagtg acataccaat agctcctgat aaagcacctt ttttacttcc
    16441 aaatactatt gctgatatga acaacattgc agttcctaaa tgcacaagcc caccatttat
    16501 agatattggt aatcttatgt ttataaattt agttgcaata aatactaatg ctattaatag
    16561 tgaggtttcc accatgtctt tcgttgtaaa tttttgctta gctgtaactt ccatactata
    16621 tccccctatc tattaaaaat aatacttatt aaataaattt aacaaatttc catttccttt
    16681 gaatcctaat gcacttaaac ttttatccaa aacatttagt acatgttcta ttttatttag
    16741 atttgcattc tctcccatat gacctattct taaaatagta tccatatatt gatttagtga
    16801 tgttgcaaca agtgtattat aatttttaag catataatca gtaagttcta aagctcctat
    16861 actttcagga atttttatag ctgtgactgt attagaatat ccttcttcta aaaatagttc
    16921 tagaccatat tcctcaatag attttctaac actataagct atcttttcat gtctatttaa
    16981 tacattttct attccttctt ctaatatatt atcaagtgct ttatcaagcc ccattatgtc
    17041 acttattggc attgtataag ggaagtattt tttttcataa tagccttccc atatatttaa
    17101 attacaataa aaacctatta catcagtttt tctatttttt atacaatttt ttgcatcttg
    17161 acttattccg actatagtaa gtcctgctgg tgcagaaata gctttttgtg aaccacctaa
    17221 tattatatct attttagatt catctacact taatctttct cctaccatac ctgctacaga
    17281 atcaactaca gttaatattc catattcttt aagtaatgga catattttgc ttacatcatt
    17341 taatacgcca gttggagtat cacaatgcac tactgttgca tatttaaaat tactatcctc
    17401 atctaaaaat gctttcaatt catcaatatc tatactttta gtatattcac ttgaaaaaag
    17461 cacatactcc ccaccataca ttgttacaaa atctttaaaa ccttctccat atataccatt
    17521 gtcaattaca agtactctat ctcctttttc tgtaagtgat gcacatgctg cttcaagacc
    17581 taatattcct tcaccactca atatgtaaac atcgtttttt gttccaatta tattaccaaa
    17641 cttctcgcat gtctttttat aaaattcaac aaaatcaata tctatatctg gatttgttgt
    17701 tttttctgct cttgctaatc ttacattttc cctagtttca gttggtcctg gtgcataatt
    17761 aatcttctta ttcatatata tcagctccta tttattttct atacgattat tatacaaaat
    17821 tttcatttta tatggtacaa ttttatactt ttttagaatt gtaccggtac attttctaac
    17881 atgaataata tcagtatatt agatatatta ttgattacat aataaaatac agagcaaaca
    17941 tctgcttaaa ttgtttgctc tgtactatgc tttaacctat ttaattatga atctctttaa
    18001 ctatattttt ttatatgttt tttgctagtt aaactaacca taaatatttg tattttattc
    18061 actgtatctt actctatcaa taagagattc tttattaaaa cttcgtccta tcaaataagt
    18121 tgttataaat tgagctataa gaatacacac aatcatcaac aagattggta ctattggcaa
    18181 agaatatgtt gcataagata atccagtctt ttttagaaat ataacagcaa tataccctaa
    18241 tatacctcca aatagaatac ttcctatcat cattccagaa gtgtaataca ttgcctctga
    18301 attaagcatt ttcactaatt gtttattagt aagaccaata gcttgaagca taccaagttc
    18361 tttttttcta gtaactatgc tagttatcat tgtgttgaca agattcataa atccaatcac
    18421 acctattatg cctgttagcg tgtatcccat tatctttata aatgaaatag tagccttatt
    18481 tgactcaatt ctactatcta taaaatcagt ctctaaaaat ccattatttt tagcaatctt
    18541 cttgatacta tctttgattt cttgatactt tccttcttct gtatatattc ccaagctact
    18601 agttgaatcg tatttcaaag ttctatcaat aaaatccttc cctataccaa aacttggaac
    18661 ccccatagct attgcttgaa ctttaaattc tttttttatc tttttatctc catcatataa
    18721 agttaatctt attttatctc ctgcttttac tcccatttct tttgctgtat caacatgagt
    18781 aattactatt tcatctccag tttgaagtct ttcaagattt atttttccat ctattatatt
    18841 cttgtttaat gattttacat cattttcatt tatactttgt aaaaggtcat acttaaattc
    18901 aacatcatag tcctccatac caatttttac acttcttgca gattctattc ttttaattcc
    18961 ttccatgttc tttatttgat taaaaaaatc tttacttaga ggattatcca tttgtaaaat
    19021 attcaaatta tctttaggat ttttatcact gttcatttca taaccagata gtctaacttc
    19081 tatatcataa ggaaagtgtt ctcttgccat tttctcagca tcaaaacttt ctaaggcagt
    19141 tgatactgtt ataaatatag ttccactaag tataagtgat gccaatgtca tgtaagtacg
    19201 ttttttattt ctttctaaat tggcatgtga taatctcttt agatttatat ttttatatcc
    19261 ttttctctta ctcttattac ttattttatt tcctgtatat cttactgcgt caacaatgga
    19321 tacttttgat gctactttca ttggttttaa aagtgataaa acaacagata taaagcttat
    19381 taccacaacc gataatataa ctggtaatcg tgatgatttt acatctatat ccataaatga
    19441 ttttattata atatttgcaa gtacatagcc taaaattatt cctattggta ttgctattcc
    19501 ggctaatatg aacccttcct taaatactat attttttact tgttttttag ttgcaccaat
    19561 tgctctaagc tttccaaatt cttgaacctt tgtaactatt gaaatataaa atatattgta
    19621 tattactaaa atactagaca atacaattac taaccctaca aatactccac ccataattgt
    19681 ttccatatct ggttttaaag catttatata gttttcattt acagctacat taatagtgtc
    19741 caatcctaca tcattagcaa tcttatttaa ttttgatttt gcttgttcta tcgataaatt
    19801 acttttatca tttaatttca catatatatt aaaatcttct tgcgacatat ctctagtatt
    19861 tctcatatat gattctgata taatagcata ataatattta cctgcttcag atatttcgct
    19921 agtttttaag attccactta taatgaactc tttttcaatt tttttatcac ttttatagtc
    19981 atcataagat atttttatct tttcacctaa atctttattt ttataaccaa gtgcctttaa
    20041 agctccacta tctaacacaa tttcattctc ttttgatggc aattttcctt tgataaattc
    20101 aaaactattt aatttcaaag catcttcatt catgtatgct agagaaatat ttgaatcatc
    20161 ttccagtttt tcaacaccta ctccattcat ttcaccagtt aattcaacat ctgcatgatt
    20221 actcaatata tcagcctgtt tttcatctac atttgctaat ataccttgat aattgcctga
    20281 atactttata gtattttctt tttgatatgc tcctgcatca acaatataaa ttccaataga
    20341 agaaagcatt aaggttgaaa gtattatagt aattattatt agaatacttt ttgatttatt
    20401 ttgctttaaa ttactttttg ctattttagt agtattaatc atttttatca ccaacctttg
    20461 acaccctgcc atcttctatg aatataactc tatcagccat ttgggctata ctgtcatcat
    20521 gagttatcat aactagagtt tgactgtact tcttagacat agattttaat atactcataa
    20581 cctcatcaga tgttttagaa tctaggtttc cagttggttc atcagcaagt attatagctg
    20641 gtcttgtagc taatgctctt gctattgcca ccctttgttg ttgaccacct gataatgtat
    20701 ttggtaaaac gtcgtgttta ttttcaagtc ctaatgattt caaaagttct tttataaaag
    20761 attcatctac ctctctacca tctaaaccga ttggcaacac tacattttcc catacattaa
    20821 gagatggaat taaattatag ctttgaaata taaaccctat ttttcttctt ctaaatacag
    20881 caagttcttc ttcttttaat gtaaatatat ttttattatc tatatacacc ttcccagatg
    20941 taggtatatc aagacctcca atcatatgca aaagtgtact ttttccagaa cctgattttc
    21001 ctattattgc aacaaattca ccttcattta tttctaaatc aacattgtcc aaagctctta
    21061 ctaagctttc tccttttcca taatgctttg ataaattttc agtatatagt attttcataa
    21121 aattatcctc tcattctaag ttatattacc ccatatttta ttaatttttt atttctggag
    21181 ctgtttatac ttatataata aatgtagagg cttacatttt cctttcattt aatattacat
    21241 agttgtaaga aacaaagaaa acttgctccc tattcctact tttgaagaaa ctataatatt
    21301 tccaccttgt tcttctaaaa tctttctact taaatacaat cctaccccac taccttctac
    21361 agattcaatt tcatcagcag aacctctata aaaacgttta aatatattat taaactcatt
    21421 tttttcaatc cctatgccat tatcctctat tataatttta aagaaattag cagtttcttc
    21481 tacagatata tttatttctc catttacctg tgtatacttt acagcattgt ctaatacatt
    21541 aaaaatactc tcctcagtcc actttttatc atgtggtata attctactgt taaattcatt
    21601 taaatttaat gttatacttt tgctcttagc cttaggggta acacttttta ctgcatttag
    21661 aatagtattt tttatatcat tatcttcttt ttttatgttt atcatgctaa tttctaacct
    21721 tgatatgttc actaaagaat ttattaaatt ttctaattta tttatagaag ccttattggt
    21781 caataagaac tcgttggctt catcttcttc taattcttct tctataagta atgtattgta
    21841 taattttaag gatgctaaag gagtttttaa ttgatgggaa atatctgtta ctaatgattt
    21901 tacactttct ttttcttttt ctaatttaat aaactttatc tttaaacttc tattaagctc
    21961 atatagacag ttatttatct tactaaatat gccttcttta aagtattcat tatattctac
    22021 tagataacca gtttcttctg actcagtagt gtccttcaag tagttttctt gtgacatctt
    22081 atctactata aaataaattt tactcaatct tttatttatg tatcttataa aataatatat
    22141 tatacctata actactataa acatcattaa aaaaataaaa aatgattcct taaaaaaaga
    22201 ttttaagtat aagctaaaat ttttatcatt tgacatttta ttttccaatc catagccaaa
    22261 tttcttaaat gccttttctc cagtagacac cttactataa tcagaattat aaatagaatt
    22321 tactatatct atttcatcat taggatattt ttcaatcaat attccagcaa tattttgagt
    22381 attttttatt tgatgttcat acaatttatg taatctgcta tattcaaaaa gtataaatac
    22441 aagggttact attagtacag ataaaacaga atatacaata actcttctca ttatataatc
    22501 atctttcaca cttaaaccat ctttgaggtt catatttttt agcatttttt tatactcctt
    22561 tctccccaca tatatccaac ccctcttact gtttttatat atttaggttc agatggattt
    22621 ttttcaacct tttgtctaag tcttcttata tttactgcaa tcgtattatc atctacaaaa
    22681 ttaccactac tatcccataa agattctaac agttgctctt tagtcaatgt ctgcatagaa
    22741 ttttccatca aatattttaa tagttttgtc tctgtcttac ttaaaattat ctcttcttcc
    22801 ttatcatctg ttcttataat taatttattt tctatataat aaaagaataa atcatcacaa
    22861 actaatagag tataattttt tactgtattt actcttttca taagcgcatt cacttttgat
    22921 attaatacca tcaagctaaa aggctttgtg atataatcat ctgcacctaa atcatatcct
    22981 gtgacaatat ccacctcttc atcaagagct gtcaacatta ttatgtaaac atcacttttc
    23041 tttctaatct cctcacaaaa gtcaaaacca ctaccatcag gtagacctat atcggaaata
    23101 attaagcata tctcttcttt tgcgaaaata ttttttgctt cttctatacc aaatgcacta
    23161 aaaacattgt atccttcttt ttttaattta aaactaattc ctctatttaa agatttgtca
    23221 tcttccaaaa gtaatatatt attcatccct at
//
LOCUS       DAGSHT010000040        23201 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.041, whole genome shotgun sequence.
ACCESSION   DAGSHT010000040 DAGSHT010000000
VERSION     DAGSHT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.041"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..393
                     /locus_tag="MJB72_001428"
     CDS             <1..393
                     /locus_tag="MJB72_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262547.1"
                     /translation="IKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASII
                     IGITLFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGN
                     LNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            380..1135
                     /locus_tag="MJB72_001429"
     CDS             380..1135
                     /locus_tag="MJB72_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8262548.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1266..2288
                     /locus_tag="MJB72_001430"
     CDS             1266..2288
                     /locus_tag="MJB72_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8262549.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2452..3009)
                     /locus_tag="MJB72_001431"
     CDS             complement(2452..3009)
                     /locus_tag="MJB72_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY8262550.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(3015..4091)
                     /locus_tag="MJB72_001432"
     CDS             complement(3015..4091)
                     /locus_tag="MJB72_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBY8262551.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(4092..4775)
                     /gene="modB"
                     /locus_tag="MJB72_001433"
     CDS             complement(4092..4775)
                     /gene="modB"
                     /locus_tag="MJB72_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBY8262552.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4924..5736)
                     /gene="modA"
                     /locus_tag="MJB72_001434"
     CDS             complement(4924..5736)
                     /gene="modA"
                     /locus_tag="MJB72_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY8262553.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKNSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSVDSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5929..6858
                     /locus_tag="MJB72_001435"
     CDS             5929..6858
                     /locus_tag="MJB72_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262554.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLADTGKTIAKV"
     gene            complement(7019..7468)
                     /locus_tag="MJB72_001436"
     CDS             complement(7019..7468)
                     /locus_tag="MJB72_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262555.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7671..8651)
                     /locus_tag="MJB72_001437"
     CDS             complement(7671..8651)
                     /locus_tag="MJB72_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8262556.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDTLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            complement(8856..9716)
                     /locus_tag="MJB72_001438"
     CDS             complement(8856..9716)
                     /locus_tag="MJB72_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBY8262557.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELIDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(10033..11361)
                     /locus_tag="MJB72_001439"
     CDS             complement(10033..11361)
                     /locus_tag="MJB72_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY8262558.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(11509..11838)
                     /locus_tag="MJB72_001440"
     CDS             complement(11509..11838)
                     /locus_tag="MJB72_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY8262559.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(11862..13151)
                     /locus_tag="MJB72_001441"
     CDS             complement(11862..13151)
                     /locus_tag="MJB72_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8262560.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(13167..13493)
                     /locus_tag="MJB72_001442"
     CDS             complement(13167..13493)
                     /locus_tag="MJB72_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8262561.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(13628..14257)
                     /locus_tag="MJB72_001443"
     CDS             complement(13628..14257)
                     /locus_tag="MJB72_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262562.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(14472..17129)
                     /locus_tag="MJB72_001444"
     CDS             complement(14472..17129)
                     /locus_tag="MJB72_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8262563.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(17488..18432)
                     /locus_tag="MJB72_001445"
     CDS             complement(17488..18432)
                     /locus_tag="MJB72_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262564.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(18425..19450)
                     /locus_tag="MJB72_001446"
     CDS             complement(18425..19450)
                     /locus_tag="MJB72_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262565.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(19525..21093)
                     /locus_tag="MJB72_001447"
     CDS             complement(19525..21093)
                     /locus_tag="MJB72_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8262566.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(21206..22117)
                     /locus_tag="MJB72_001448"
     CDS             complement(21206..22117)
                     /locus_tag="MJB72_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262567.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(22101..23087)
                     /locus_tag="MJB72_001449"
     CDS             complement(22101..23087)
                     /locus_tag="MJB72_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262568.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
BASE COUNT         7509 a         4021 c         2627 g         9044 t
ORIGIN      
        1 ataaaaatac taggacttat gatttcaaat ggacttatag ctttatctgg tagtcttatg
       61 gctcagttct tgggattctc agatgtaaat atgggtatag gaactttagt tcttggaatt
      121 gcctctatca taattggtat tacactattt aagaaattta cttttatcaa agatactact
      181 gcaataattg ttggctcttt tatataccaa ttcactatat actttgcaat gagtttagga
      241 atgttatcaa ctgacttaaa actaataact gccatagtta taatagcttt cctagctact
      301 ggtaacttaa atatttcact aaaaaaaaac aaatgcaaag ctagtaccaa aaataaatca
      361 gaaaaaagag gtgttgtaga tgttacaaat taaaaatctt tcaaagagtt tccctaatcc
      421 atatggtgaa cctaatacta tctttgaaaa tttatcaatc gatatagaag atggtgaatt
      481 tgtaagtatc ataggtagta atggaactgg taaatctact ttattaaata taatatccgg
      541 gcttataaaa gaatcttctg gtcaagtatt attaaataaa cataacttgt ctaatttagc
      601 tgaacacaga agaactcaaa tagtaagtag agtttttcaa gacccaagtc ttggaacttg
      661 cccttctatg actgttagag aaaacttgtc tttagctctt aacaaaggaa aactattaaa
      721 tctaagaaaa tgtcttcgtt acaaaagaga tttcttagaa aatttattgg aaggtgtatc
      781 tcttgattta aaaaaatacc ttgatgttca agtaaaattt ttatcaggag gtcaaagaca
      841 atcattatca ctaattatgt cgtgcttaac aaatccatca gttttattac ttgatgaaca
      901 tacagcagca cttgacccaa aaacatcaaa tgaagtaatt gaattgacta ataagattgt
      961 aagggaaaaa aatataacta cacttatggt tactcacaac ttaaaacacg ctcttcatta
     1021 tggtgataga ttgataatgc ttcataaggg tgaagttgta ttagatgtaa aaggtaaaga
     1081 aaaagaacaa ttaacagttg aagaaatctt agaaaaattt gaatatgcag tttaaaataa
     1141 tatatctatt gtattaaact cggctttaaa ataaagttaa aattacaata attatggtta
     1201 ctttgtaata attcatatga atatatgaac atatataata ataaattttt gagggggata
     1261 taaaaatgat taataaaaaa agattggcaa gtttgatttt agctggtgct cttagtatat
     1321 ctatgcttac agggtgttca caaggaggag actctggtaa ttcaaaacaa gaaagtaatt
     1381 ctaaaggtaa agaagttaaa aaaattggta taactcaact ggttgaacat ccagcattag
     1441 atgccactag gacaggtttt gtaaaagcat tagaaaagaa cggatttaaa gatggagaaa
     1501 atattgatat agacttccaa aatgctcaaa atgacatgcc tactacacaa agtattgcga
     1561 gtaaatttgc atctgataag aaggacttaa tatttgctat atctactcca tcagctcagg
     1621 cagcatttaa tgctactaaa gatataccta tacttattac tgctgtgagt gaccctgttg
     1681 ctgctggatt agttaaaact cttgaaaagc caggcacaaa tgtatctgga acatcagatt
     1741 ttgtttctgt agacaaaggt ttagaacttc ttaaaatatt tgctcctaag gcaaaaacta
     1801 taggagttat gtacaatact agtgaagtaa attccaaagt tcaagttgat gctttaaaag
     1861 aatatgcatc taaaaatgga tttaaagttg ttgaaaaagg tataactact tcaaatgaag
     1921 ttaaccaagg tatttctagt ttagttggca aaatagacgt attatatgtc cctacagata
     1981 acttagtggc ttcttctatg cctatagtct ctaaaatagc tactgagaat aaaatacctg
     2041 ttatagcagc tgaatctggt ccagttgaaa aaggtgctct tgcttgtcaa ggtatcaact
     2101 acgaaaaact aggttacaaa acaggtgaaa tggcagttaa aattttaaat ggcgaatcag
     2161 tatctgatat gcctgtggct acatcagatg atacagatat cattgttaat gaagatatat
     2221 taaaagcttt aggtatggaa aaaccttcta atgaaaatat ttcttatgta aagactaaac
     2281 aagaatagaa agatattttc tatatacgat taatatgcct gtttctataa attaatttat
     2341 agaaacaggc atatattttt attttaatgt attatctatt taaagattat atgtaaatgc
     2401 tcttaatttg tttgtataaa ttttacttgt ataaacttta tacaatcata tttattcttc
     2461 tattttcttt attactctac atggatttcc aacagctaca gtatttggtg gtatatcttt
     2521 tgtgactaca cttcctgcac ctattactac attatcacct atagtaattc ctggtgttat
     2581 gattactccc ccacctatcc atacattatc acctattttt acaggagaac catactctat
     2641 acctgaattt ctaagttgtg catcaattgg atgataggct gtatagattt gaacgtttgg
     2701 agctaacatt acattgtcac ctatttcaat tttacataca tctaaaaaaa tacaatcata
     2761 atttgcaaag aaattttcgc ctacatgtat gttatatcca taatcacacc taatgttttg
     2821 ttctacattt atttgcttgc caactgaacc aaatagttgt cttaaaatat cttctctttt
     2881 ttcatattca tcctctaaag tattattaaa caatctagtt aattttttac aatactctct
     2941 ttcttttact aacaattcat catttgcata gtatcctttc ccagacaaca ttttttcttt
     3001 ttcagtcata tagtttactc ctttttgtta taattgtatc taaagcaaaa taaatattcc
     3061 tgtggaaaat ctaaatatat tctgtcacct ttcttaaatc tcattttact tttttccaat
     3121 tctatttgaa taggtacaca ttcgttagat aaatcagttt ttctaacata tatagtatat
     3181 gtaaaaggat tttctacaat gttaatcaat tcgaaacata acttttcatt taaatcttca
     3241 ttagaatcca agaccttaat atgatgctcc ctgattccca catattctat attatcacct
     3301 atttctctat ttaatgtcaa ctcgcatccc cagtctgtag cataaatagt atttttatcc
     3361 agtctattta atttggatat atttttacac cctgttatct tagcttctat aaggtacttt
     3421 ggagattcaa agagctcttt tttaggtctc tttggaagac cttctccttt gttgtacact
     3481 ataatatcat cacaaactcg gtaagcttct tcaatatcat gagtaacaaa tagtatatct
     3541 cccttgtaat cttttaacat attcataagt tctttttcca tattgcttct taaatgatgg
     3601 tccaatgctg agaaaggttc atctaaaagt aatatatctg gagatgttgc caatgctcta
     3661 gctagtgcta ccctttgttg ttgaccacca gataattgcc aagggtatct cccctcaaac
     3721 ccatttaatt taagtatatc taagtacctt gctactattt cctttttttc actcttactt
     3781 agttttaaaa gcccaagttc tatattttga cttacagtca tatgtggaaa caaagcatag
     3841 ttttgaaata aaaatcctac ctttctatct tgtgttgata aatttatctt cttttcagaa
     3901 tcaaatagaa ctctatcatt taatacaatt ttacctcttg ttggtttctc aagccctgct
     3961 atacatttta atgtcatact ttttccagaa ccagattctc ccaaaaaccc cagagtacct
     4021 ttttcttgtt taatttctac ctttagttta aaagaggata aatctttttc aatatccacg
     4081 tataagctca actatccaca cctctttcct ataattttct gctgaaattc cgaccaataa
     4141 tttagtaata aaatcattgc tatagatatt gctacaataa tcataaccca catcatagct
     4201 ttattcatat ctccgccttc aacagcaaaa aatatagcta ttggcatagt ttgagttttt
     4261 ccaggtatat ttccagctat cataagtgta gcaccaaatt ctccaagagc tctggcaaac
     4321 gacagcacca atccaccaat tattccaggc catgctaatg gaactgcaat cttaaaaaat
     4381 actttccatt cattaagacc taatgttcta gcagctgaca acatattatt atcaatctgt
     4441 tcaaaagctg accttgaagt tctatacatc ataggaaaag acaccactac tgctgaaatt
     4501 acagttgctg tccaactaaa tattaaagtc ctatcgaaat ttaataaaaa ctttcctata
     4561 aatccattct tcccacatag taataataaa aagaaaccta caactgttgg tggcaaaatt
     4621 agtggaagtg taaatagtcc atctataaga cccttccact tacctctata gttagccata
     4681 atatatgaca caaatattcc tattacaaaa gttattattg ttgacaaaat agatgtctta
     4741 agtgatatcc ataagggtga ccaatctgtt cccatctttc cctccttaat tttaaatgga
     4801 aatagtgaaa ctaataactc tattaatttc actatttcca ttttattata atacacaatt
     4861 attttcaatt ttcatttatt tatatataat ttatacttaa ttacacataa tttatttcta
     4921 agtttattct acttttttat atccaaattc ttcaaaaatc ttttgtcctg actcacttaa
     4981 taaaaattct tcaaatttct tagcagcatc tacgttttta ctagctttaa ttactgaaac
     5041 tggatatgta ataggtgaat gagttttttc gtctgttttt tcaactactt ttatcttatc
     5101 attattgaca gtatcactga agtaaacgaa tccaacatca gcatttcctg attgcaccca
     5161 tgctaaaact tcttttacat cttttgcaaa tactagctta tcttttaact tatcttttaa
     5221 atttaaatta gttaacacct catctgcata cttaccagct ggaacacttt cagcttcacc
     5281 aacagcaatt ttcttcactt tatctgtagt caaatctttc atacctgata cactactgtc
     5341 aacagaagaa ataagaacta aatcattttt tactaaatct ttcatagtat cagaaactaa
     5401 taacttctct tcatccaaag ctttcatttg tttttgacct gctgaaataa ataaatcaca
     5461 aggtgctcct tgttctattt gttgttgtaa tgagcctgat gctccatagt taactgtaag
     5521 ttttacattt gaatcaactt ttttatattc ttcttcaatc tttgccattg cttcttttaa
     5581 acttgcagca gccgatatat ttaactctac tgaatcacta tttttagttg ttttttcttg
     5641 ttttccatca tcttttttgt cttcattact attgcaccca actgtcccca aagttaaaca
     5701 tgcaactaat cctaatattc ccaatatttt tttcataaca atctcccttt ttgtaaattt
     5761 gatacacatt ttaacatgtt ttattatgtt ttatcatgtt ttgttatata tagtttaatt
     5821 atatatacat gataataaat tttcaagtaa tttatttaca ctacttttaa atattttgct
     5881 acattttcct ttttttaatg ttaaaataac cttatatgga ggtggattat ggactcatta
     5941 tcatctttga cacctgtcga ggtagctgaa ttattaaaga taacaaaaaa tactgtatat
     6001 gaattaatca aaagaggtga acttccttct tataaagtag ggaaaaaaat aagagttgat
     6061 actaaagatg ttgaagaata tatcaacagc caaaaaactg gtaaagtaaa ggatacacat
     6121 ttcaacaata aaaatataga tataaataat aatttcaaaa ttattatttc tggtcaagat
     6181 gtaatattag acattttagc taggtctgtt gaaaaaaaat tggatggaat aagtacattt
     6241 cgctcttata taggtagtta taatgctttg tatgagttat ataatggcag agtttctata
     6301 gcatcatccc atctatggga tttcgaaacg gatgaatata atagcacttt tgttaaaaag
     6361 ctattgccag gaataccatg tgtacttata aatctagctt atagaatgca aggtttctat
     6421 gttgctaaag gaaatcctaa agagataaca acctgggagg atttaacaaa accaagtata
     6481 accattgtaa atagggaaaa aggttctgga actagggttt tactagatgg taaacttaga
     6541 cttctaaact ttaatggtaa acatataaaa ggttatgata atgaagaatt atcacatctt
     6601 ggagttgcaa gtactgtttc tcgtggaata gcagatgttg gtttgggaaa tgaaaaagct
     6661 gctttacaag taaataatat cgactttatt cctcttcaca aagagcgcta tgatttagtt
     6721 attaaaaaag aagacttaca aaatccagtt taccaaacta taattaacat aataaactct
     6781 cctgagttta aagctgaact tcaaggtctt ggcggatatg atttagctga tactggaaaa
     6841 actatagcaa aagtttaaga ttaaaatcgc aatctatttt ataagattta taatttttca
     6901 caaaatttat aacttaaaca cgaaataaat tgcgatttta gtgtctcaaa aaatttatgt
     6961 ctttcatttt atcatccaat acaaaatata atataattaa ttgtgcaatc atattatatt
     7021 aatatatatt tacataatag tatttttctt taatgtagat taacatatga tatgttttaa
     7081 acatcaatcc ccttgctttc ttatatatat gtggcaacac atatgagttt aataaattat
     7141 atacaatgtt gctttcttct tgtatttttt caaccaacaa gttatttata aaattacctt
     7201 tctcattttc aataatctca ctccaattta tcttaacttt taatatatat cttcccacta
     7261 ggtatacaca ctctacaata aataataaaa atacaatgca gtcagtacct gttgtcttat
     7321 ataaattacc cgatactaat ggctttgcga agaaattata aaaattcaaa gtatcacccg
     7381 tataatcaaa aagtattaat agcactccta gaactctcaa tactttaata ttcaacatgt
     7441 ttttatcttt attatttttg tttttcatat atactacctc tcttttcttt agatttgatt
     7501 gtaatctaat actaactctt ttatttgagg tataaatgaa tttaattggt agttattttc
     7561 atttttacta gaaattaatt cacattacta aacaaaacat atatttaaac ataaaaatag
     7621 cctatctatc ttttaataaa tacaaaaata aataagctac taatattcaa ttaactttta
     7681 tcacttaaaa cattactatc tataaattta aatattttat tgaagtatac tgtactatca
     7741 tgttcaaatc catcaacatg accaatacct tctataagcc ataggtcttt tttatcatgt
     7801 ttaactaaat catacatttt cttgctattt tgataattac aaacagtatc ttttgttcca
     7861 tgtataaaca gtatgggtac attagatttc tcaaccgact catatggttt tacatcactg
     7921 taccaaaatc cagatttaaa cttattatat aaatctccac aagtcaccac ataattgatt
     7981 aatatatctg gtattcgttt ttctagtact cccattctaa ttgcatcttt catttcagaa
     8041 taaggagaat ccagtatata aaaatcgacc ttatcatctt ttgaatttat ctctgaatgc
     8101 atagcagctg tccctgcccc catggaaaat ccatgcactc ctaatcttcc ttcaggaaat
     8161 ttatttttta caaatttaac tagagtatct aaatcatacc tttcataaag accaaatgtg
     8221 taattatctc caccactatt accagtattt ctttgattat aaactaatac attataacct
     8281 ttatctaaat atctatatgc tacctttaac atttcatagt aactacttcc aattccatga
     8341 acaagtatca tagtatctct tgtctttata tttgaagtta taaaaagact ttcaagcttg
     8401 taattattag aactttgtat cattaattca gatgtcttgt agttttttaa tttctccagt
     8461 ggtctatctt ttctctcaga aaatattttt actatatctt cgttttttat taactgctct
     8521 aaaccaactg aactattata cactaatttg ccaaatatat aagatacaat taataggatt
     8581 aatagaaaca caattgatat aattataaat ttatttctta aactaataca atttttaaaa
     8641 ctatttttca tatttattta ccatacaatt ttcctatatt atattttcta agtaatagca
     8701 taattgatta tactattctt ataatttcta ttatacctac ttatttatta aaaatacaaa
     8761 ttatatttta ttaattttta ctacttgtaa attattatct tatacaatga atttgtatta
     8821 tataataagt atttttagga ttctgttaca taatattatt taaatattct tctatatttc
     8881 tcttcttctt cttcagtaaa cacattaaat acaactttta aatttttttc aggattttct
     8941 tttagccata gtctaactct acttacagca agattagctg cctctttttt tggataacca
     9001 aatacacctg tagaaattcc acaaaaaaca atatttttta tttcatcaat ttcttttatt
     9061 gtatttaaac atgatttata acaatgaagc agttgctttt cttgctcttt acttggctga
     9121 ccaccagata caataggtcc aacagtatgt actacaaatt ttgcaggtaa acaataacct
     9181 ctagttatct tagcatctcc agtatattct aaatgccctt gtttttttat tattttgtca
     9241 caatcttctc taagtctagg acctgcgcat gaatgtatct cattatccac gcatagatgt
     9301 aatggttgta aacaccctaa taacttgtta tttgcagcat ttactattgc atctgctctt
     9361 aaattcgtta tattacctct ccatatggca atcccttcct ctacttcttc tatatcattt
     9421 acatctatta actctctttc ttcacattca atcaacaata aattatgtaa catattaagt
     9481 atctctggag caatttcttt tggtggatat acattcaaaa gtcctctaag tatatctcta
     9541 ggcttaattt catcatttga taaattgtat attttagaag gaacttcttt tgaaaaatat
     9601 gcaattaaag tgtttatatt ttttcttctt tgttcgtcag ttagaggctt gatagtttta
     9661 tcaaaatcct caaataaatt tacatcatca gcataatctc tccacttcat agccatctct
     9721 ccattcttgt attttagact taaaaataat tatgtattta tgtacatatt tatttatttc
     9781 tataaacacc tacataaatc ctgttaaaag tgtaaatttt ttattatgca acttctctgg
     9841 ttatttctat tgattgatag taaattattg tatctaaatt taatccaact aacaagtaac
     9901 taaacctact ttcctaactg caaaatacac tatgttttcc acttgtatct catttgtaga
     9961 taaataaagt ccctacaaat acattaacta agctatgtac taaactgtag gaactttaaa
    10021 aatatattat tcttaattaa gttttggcca ataattttta tttgcttcta ttaaatcatc
    10081 tagtaattct tttgcaactg ttgcactagg aactgtctta gatagcataa gtgcttgcca
    10141 caatttatta taagacttct caatccaagc atcaactact aatttttcaa ctgctaattg
    10201 ttgttctatt aaaccttttt ggaatgttgg tattttccct attgataatg gttcataccc
    10261 gtcttttcca actatacatg gaatttcaac catagcatca tcttgtatat tttctatagc
    10321 tccattattt tgaactatca ataacatacg ctcctgtgta ttaaaagcta gagctgtagc
    10381 taaatccact ataaactctg catgtattcc tatcttaagc ccactgccct tagaatgtcc
    10441 ttgcttttct atcaatgcac atgaaccaaa aacttctttt tctcttccat caataacttc
    10501 attggcacga gtataattta catcagaatg atttacaaca taatctggaa atagatagta
    10561 ctttaagtaa ctacttggta ttgtagttaa atctgttttt actatttctt tagtaaataa
    10621 attagtttca aaccaactat ctttcttgtc atttttcatt ccttcttcac catttataaa
    10681 accatattct tttatatgtt ctactaattt tggtaaaagt tcatttcctt ttttatcttt
    10741 aattgaagta taccaaccaa agtgatttaa tccaaagtat cttatatcca tatgtttacg
    10801 agatttgagt cctgcaatcc ttgctatatt atgttccata cctattggca tatcacaaat
    10861 atttattacc ctagaattag gtctcaatct tctaacagct tccgctatta ttgatgctgg
    10921 attagaataa tttaacatcc aacaatttgg agaatacttt tccatataat ctatgttttc
    10981 aagaacacct tctatagacc tcattccata agctattcca cctggtccac aagtttcttg
    11041 accaactact ccatgtttta gaggtatctt ttcatctagt tctctcattt caagttttcc
    11101 aactcttata tgagctaaac aaaaatctac atctgtataa gcctctttag ggcatgttgt
    11161 tgctaaatac tctatattag ggtccttttc ctttattagt atttcacaag ctttagctac
    11221 cttattttgt ctttcttcat cattatcata taatttgatt gctcttaatg gtaacctatc
    11281 taagttatct agcaacatta atatgatttc tggggtatat gtacttcctc ctccagctat
    11341 tactactgaa aatttttcca taataatatc tccttttcta tttaaaaatt tactgtattt
    11401 ataaataaat ttataaatac tagtacttta aagtaaaagg gagttcattt attaaatcta
    11461 ttgatttaat aaactatagc caaacttcaa gcactattta caagaaattt aattcttatt
    11521 aattcttggg tccatcatct caaatatatc tacaagttca gatactaaag aatgagcaaa
    11581 taatgcattt gaataatggt cttcagagtg cacaactaaa aaatcagtcc cactatcttc
    11641 actattcata gcatttgata gtaattctaa atgatgcttt gatgctgttt gtattttctc
    11701 tgaggctgaa tttaaaaggt gttttgcttt attgtagtca ccttttttta tttccttaag
    11761 agacatcatt acatcacttt tagcatctcc tgcttctgat attatactaa atactttttc
    11821 ttccatttca ggtaccatat ccttctcctt ctaatgactt gttaaaaatc aaagctatca
    11881 tcatctaatg ttgtagctaa ttctgcattt ttatttaatt cttccttctc cattatctta
    11941 aagaatggat aatatatcaa ggtcataagt attatacttg caaatactaa tacgattgcc
    12001 ttccaatcta atgtcatcaa aaatgctgca acccctggag gtaaaaagcc tggtggttct
    12061 gcaataggta atcctactaa tcctactttt aatgccataa aattaagtgc tccaaccaat
    12121 acaggtccaa atacaaatgg tacaaacata tatgggttca taactattgg tgttccaaat
    12181 attactggct catttattcc aaatagtgat ggtaaaatca caacttttcc catactatta
    12241 aaactcttac acttacttct tgacataagg tacactaatc ccaaagttat ccctgaacca
    12301 gttacttgca agaaaccata gtaaaatcct attgtaaaca catattttgg gtctcctcct
    12361 gctgctatca gtgttgcatt ttctgccata tatgtagctg ctattggcat ccatatactt
    12421 gaagttattg ctgctccatg aagcccaaag aaccataata attgagttat aaatagtatt
    12481 atagttactg caaaaacatt atctacactg cttatagctg gtgctaagaa attcattata
    12541 atagctggta gtggttgacc tgagacattt ttagttattg ctgctattgc tgtaaataac
    12601 attatacata ctatcattgg agttaagttt ctaaaacttt tatttactac actaggtact
    12661 tcttttggaa atctaattac gagattttta tcattcataa acttagtaat atcaactact
    12721 aaaagtgcta ctaatatact tacaaataaa ccttgtgcat ctaaataact tgtatctaat
    12781 acaccatcta ctacttgtcc acatacaaca agttgtgcaa caactgctgt catacctatt
    12841 tctacatgat taagattata ttttttagct agtgcctgag ctataaaaaa tgctgaatat
    12901 atagataata aactcatagt aaatttatta gggataagta atgtatccat attttcagat
    12961 aataatgttt ttgcacttcc ttcaggtaac atattgttta atgccataaa taacatactt
    13021 aaagaaccaa caattattat tggggtaatg cttatcatac cttcttttat agcttgaaga
    13081 tgcctttgtt tttcaatctt ttgtgctatt ggaagaagaa atcttcttaa attattttga
    13141 agtgcttcca tttttattcc ccccaattat ctatttttat ataattcgat tgccgacttc
    13201 ataacagaag gagcatccat acgaccataa tgcttagtat ctattaaacc aactgctatt
    13261 ttgtaattct tacaagcttc aactacatca tcttccttaa acctaatttg tggtgctaca
    13321 agtactacat ctgtatcatt agcttcaatg tatgatttta aattttcaaa tgatgttgcc
    13381 tctatattta tagttttagt ttttaacttt tcatgctcac tacaaaaact tttcatcttt
    13441 tccacaagta cacctgttga agctccattt gcacatacta ataaaatatt cataattacc
    13501 ctcctacaat atctaacctc ttttgttttc taatttttta tcagccaatt cttactctaa
    13561 ttcctatcag tttttacttt aattcttatt attttatttt aattcttatt aatatttact
    13621 ctaattctta gtactgccaa tgattaaact tgaaagccgt atctataact tcttcttgct
    13681 tatatttttc taatattttt aagtatcttt ctttataatc attatctact tctttttctg
    13741 gtattactat actagcttca tttaaaaagt ggaatgaatc ccttcccatt tctaatcctc
    13801 tatatgtatg cttgtcaagt gccacgtcca taacttctgg aactttaccc attgcaaatc
    13861 cttttataat tatatttttt aataaatcac ttgatctatc tttctcacat gcacaaagat
    13921 atcttattgc atgtatgaaa aataaaggtc tgtctccatc tgcatatgaa tattcctttc
    13981 ttacttgatt taaattattt atgattatta cagctgatgg gtcccccatt cctatatctt
    14041 caactgatat tgtaagtatc cttctccata gcttatcttc taattctggt gatgatatat
    14101 atagttcata agcaaattca caagcttctt ccaccatatt tcttcttata gctttttgaa
    14161 atgacgatat tatttcgtct gcactaaatc cgtttcttgt agtcattctt gcccatgggt
    14221 catttgcctt tttatctctt aatattttat cttgcatatt atttactcct ttttatttaa
    14281 attttaatta tttatattgc tcatttaata atataagcaa gtattatgcc agttttttta
    14341 gacactttaa aacactgtta tttcaatcat ttttatcatc aaaacttaga tactttgtct
    14401 aagtatattt atatgtgtct aatattaata ttattttaga ctaaaaaaaa ttaccaaata
    14461 agtttttata ctcattggta atctttttcg attatttctg catattctaa tattaataat
    14521 atttgatagc actctttttc attaatttct atattatatt ttttatctaa ttgagatact
    14581 atatctttta ttttgcttag actcttgtga taatcaagtg ttttttcttt atcaagcttt
    14641 tcaatttctc tagtatttcc aatcaaccca tctaccatga aagctaaatg catcataagt
    14701 ccaattactt tatcgtattt cttttctata ttaaatacta aaaacaattc atcagacaca
    14761 tattttatat ctttaaatat cctattataa ttagagcttt ttaaggatgt ctttaaacct
    14821 tcaactacat catcataacc tatacttatc tttataatat tttttaattt attgatattt
    14881 tcatccttat ctatatcttt tttgctaata tagtcaattc ctctaatatt aattctttgt
    14941 tcattgatta ctgcaacaat attactactt tctttaagtt tcgtaatatt taaacttaaa
    15001 tctttttctg ttttatatgg gacagatata atatctattc catctaagta cctactcatc
    15061 ttttcttcta atatttttct tgttttatta tctacacctt ctgaaacatg tgatgctata
    15121 ataattgttt tagacagatt ttctttattt tgaatttcat tagttccaaa atagtacttt
    15181 ctcgtgttag ttatagagtt gtatacgtca tttaaaggta atcccatatt ggccttctga
    15241 actgcttcta acacagttaa tgtcgtcacc ataggaatta ctcttactcc acactttttt
    15301 tctttcttta taatttcatc aaaagagttt aatgaaccca tatctgtaaa tagtaataca
    15361 tctttattgt ctatattatt aattaaattt ttaaaatcta caattacttc atcatagctt
    15421 ttatctaact tcatatcgaa agaaatagca ctgcactttc caataatatc accaactgct
    15481 tttaccatac tacttgctgt agtttctcca tgcatagcaa ctataatatt aatgttattc
    15541 actattttat tttgttcatc ttgtttagct tgtactatga ataatgtaat atatgctaca
    15601 gtttctatag ataaaaataa atcaaattca ttttcaatta ttccaactat ttttaatgct
    15661 gttttaaatt cacttctgtt atttcttcta atttgattca aatctatgtt gtttgtagag
    15721 ctacttttat ccaatacgct ttttatataa attgccattg acttaaatat tgattcactt
    15781 atgtctattt cttcatcttc tttaataatt tgtcttattg attcacacat gcttttaaac
    15841 ttaacattat taaatagaga agcatctgct atattcattt ctacagattt attaagatta
    15901 gtatttactg cattaataat atttatatca ttgtagttaa taacttcttc ttctcctaat
    15961 gacgtaaata caaaatattc tattccatgt gttataactt catctgcttt aatagacttc
    16021 tctctataat catcactcac ttcatttata gatttatcaa aatatttttt ctctatataa
    16081 actggttctt tattatctct tttatatctt agatatccat ttgcaacagc taattttata
    16141 tcattttgaa gttgacctac attattttta caatcataaa gtaatagtga tttaattatt
    16201 tctttatgta cttttattga aatagaaata ctttttgatt cttccttgaa aaaatttttg
    16261 actaattcca ttctttcaat cattgtccta tctctcaatg atggtatttc tattttcatt
    16321 ggtattcttc tattgaatgt atctaatagt gttgatgtcg gattttctgt agtagcacat
    16381 attattaata ccttagactc tctttctttc gttgcatctc ctagtctttt aaatgttccc
    16441 ttatctataa aagtaaacaa catttcttga ccttctgctg gtagtctatg aatttcatct
    16501 aaaaatagta tccctccatt tgcaatttcc aaaagacctt ttctggaaga ttctgcacct
    16561 gtataagctc cttgttctac cccaaaaata tgtcccataa gtaattgtgc attattagca
    16621 taatcagcac aattaaatgt tacaaaagga gctttatttt ttctaattcc actatctata
    16681 gaataatcgt acatatatct agcaatcata ctctttccta ctcctgtttc tccatagata
    16741 atagtatgaa gtccatttgt aggatagagc attgcgacct tgcatttttc tattataggt
    16801 cttaaactat gttctttacc tattagtgtt tcaaaactat ctttttttat tttgttttct
    16861 acttttgttc ctttttttat gccaagcaac tcactttgtg tatcaagcaa ttcattcttg
    16921 ttaaggttaa gatttgcagg gatataatac acaggtcttc cttttatctt cgtaaccata
    16981 ccctcattga ataattggtt taaatatgta cttatattgt ttcttgcaat tcctgtcttt
    17041 tctgataatt ggttagctgt aatccctttt tcattttccc tatatgatga ttctaaaact
    17101 ttcattactt tatctttttt atatatcatt acaagctccc ctacattata gtattttatt
    17161 aactaaaacc cctatacaca ctttattttt taataaactc ttattcttta actaaggtta
    17221 taatttatca aattttcctt actttaaatt atagaaaatt attgtagaaa aaataagatt
    17281 attgctcatt ttttctacat tactattttt aattttatag tactttctta acaacatact
    17341 ttacttgttt ttaattttat tatatttgaa tttattatat ttgaaaagta gaaataaaaa
    17401 accctatttt ggagtagttt taaaatttct ccaaaaatag ggttttagtt tgtatttcaa
    17461 ataaatacct gttcaagtat ttttatacta aacaactttt aaattttgtt gatatttatt
    17521 tatttctgat ttagaagaat atacaaaatg tccatctgaa acctcaatcc aacttaatga
    17581 cttttcattt gaacaatcat atccattaga attatactct attctatttc ttgacttagc
    17641 atatctaggg tcaggaacag gaattgcaga aagtagtgat tttgtatatg gatgaatagg
    17701 attattatac aattcatttg aatctgccaa ttctactaat tttccattat acataactcc
    17761 tatacggtca ctaatatgtc tcaccataga taaatcatga gctataaata aacaagttaa
    17821 attcttttct tgttgcaatt ttttaaataa atttactatt tgtgcttgta tagacacatc
    17881 caaagaagct ataggctcat cagctattat aaattctggg tctactgaaa gagctcgtgc
    17941 tatgccaata cgctgtcttt gtccacctga aaaatcagat ggatacttat ttgcataact
    18001 tctatcaagt cctactagct ttagcatctc atatacttta ttcattctat ctgttttatt
    18061 tttacagata ccttgaattt ttagtggctc tgaaattata tctgcaattg tcatacgtgg
    18121 atttaatgat gaagtagaat cttgaaaaat aatctgcata cttttgttta catctttttt
    18181 tataaatttg tacgtttttt tatctgatat gcaatttcct ttgtaaatta cttctccacc
    18241 agtagattca tgtagtctta ttatagtttt accagttgta gattttcctg aacccgactc
    18301 tcctaccaat ccaaatatct cacccttata tatatcaaaa cttatatcat caactgcttt
    18361 tacagtagta cttttgtcca aatgaaaata ttgctttaag ttttttattt ccagtatttt
    18421 ttctctattc attagaaatc acccttccac aatttactct aactggtaca tctacttcag
    18481 gtgcatctgg gtgcaataac caagttgctg cactatgtgt atcattaatt ttaaacattg
    18541 gtggctcttt ttcataatct atcttaagtg catttttatt ccttattgca aaagcatctc
    18601 cttttggtgg gttcaataaa tttggaggca ttccaggtat attatataaa tagtcatcct
    18661 gcgaatctaa agtaggtaat gaacccaaca acccccaagt atatggatgt ctagggtcat
    18721 aaaatatatc ctctacagtt cctatttcta caatctttcc tgcatacata actgctattc
    18781 tatcagctat agttgcaact actcctaaat catgagttat aaatattatt gacaaaccta
    18841 ttttatgttg taaatccttt attaaatcaa ttatttgagc ttgtatagtt acatctaaag
    18901 cagttgtagg ttcatcagca atcaatacat ctggattaca ggcaagtgct atagctatta
    18961 ctatacgttg tctcattcca cctgaaaaat gatgtggaaa ttgtttaaat cttttctcag
    19021 gattatcaat tccaaccaat tcaataagct ctatggctct cttttttgct tcacttttac
    19081 ttataccttg atgaatgatt acagcttctg caatttgctt tcctattgat atagttggat
    19141 tgagagatgt catagggtct tgaaatatca ttgatatatt ctttcctctt atatcttcca
    19201 tctctttttc agacttctta accaaatcac ttgatttaag taaaacctct ccatttttaa
    19261 tatatccatt gtatgggagt atcctcatca aactcttaca cagtacagat ttcccacaac
    19321 cagattctcc aactatagcc aatgtctcac ctttttttag attaaaagat acatttctta
    19381 cagcctgaac ttctccttct tctactttaa aacttacaga tagattatta acctccaata
    19441 aatgttccaa aaaccatcaa cccctatgct attcaaacct tagggatata tttttatccc
    19501 taaggtttct aattaatttc agtattattc aattgtccag tcagctatgt tccagaatat
    19561 acctacacca tgatgaccta aaactgtatc aggtgttaat ccttttatgt ttggttttcc
    19621 aacatatatt gcatctatat aagcaataaa tgtataaggc atatccttag tcatttctac
    19681 ttggaattgt ttatataact ttaatttttc atctttatct tctgtttctc ttgctttttg
    19741 aagtatttta tctatagtag gattagagta agcactgtaa tttgcacctt tatctgttcc
    19801 aaatacttta tatgtatggt catctgggtc aaatggactt ccccaaccta ttaaatgagc
    19861 atcttgatta gcccaatctg tttcagttac aacaacagct ttagcatcaa caccaatttc
    19921 ttttaactgt tgtgcacata ttttagccat atcaactcta acttggtcac tctcacctgc
    19981 tgttatctcg aaagctaact tagtaccttc tttttcatag attccatcag aacctaattt
    20041 ccaacctagc ttttcaattt cttgtttagc tttttctgga ttatattcaa acttttctat
    20101 atcaggatta ttatatggtc ccatttgtaa aggtgaatat gctggtacac catgtcctaa
    20161 taatacactg tcaactattg cttttctatc tattgcatag cttaaagcat ttggtaatcc
    20221 tttagctttt ttatctttaa agaatttaga attaaaattg tataatatac ctctatagtc
    20281 ggctgttttc attatattta ctttaaaatt cttttcgtct ttttcaaagt tagacatatc
    20341 tttaggagtt atttgagcta ggtctaattc tcctgatttt aattgcattg ccttagcctt
    20401 atcatctgga acaatcttaa agactacttt atctaatcct ggttctttta caaaataatc
    20461 actattctta acaagtgtta tactttgacc tttgtcccat ttttcaagtt taaatggtcc
    20521 agtacctatt ggtttttgat taaattcatc agtagctata tccttacctt ccaaagcatg
    20581 ttttggtaat actcctactg ttaaataatc aagcattgct gtatttggag cttttaaagt
    20641 aatttttata gtattatcat taaccacatc aatttttgtt atatcttcat aatttgatgc
    20701 tatctcagaa gcattatctg gattcattat agtttctatt gtaaacttaa catcatttgc
    20761 tgtaaatttc tctccatcat gccacttaac atcatctctt aaattaaaag tatatgtatt
    20821 tgttgcctca tcaaatttcc agtcctttgc tagacaagga accaccttat tattttcatc
    20881 atgagctgtt aatccattaa atattaacga atttatttct ccatgttcgt atagtgcagg
    20941 atttatactt gtatagtcat tacttccata aacaagcacc tttccatctt taggcgtatc
    21001 tgccttctta tctttgtctc ctccactaga acaacctgcc attagactaa gtatcattac
    21061 taaacttaaa acttttaact tctttaattt catgatgctc ccccttttgc tttttataaa
    21121 atatttttca aagctttgac cccatttatt taaatagata tttcaaatac ctcagtattt
    21181 gcttatttaa tactcaaatg ctgatttata aattactaga ttttttatta ttattctttc
    21241 ttatataatt acctatattt gttatacaca ccaatgtggt aaccaaaaat atacccggaa
    21301 tcagtataat ccaccatcta tttgaaagta atgcttcctc tgacaaagat aacatacttc
    21361 cccaagaaac aatttctatt ggtaaaccaa ttcctaaaaa gctaagtgtc gattcagtac
    21421 caatagctgc gcctatgttt gttacaacca tgaacattat tgatgccaca aaatttggaa
    21481 gtaaatgttg ctttaataca tagaagaatc ctcctcccat gctcttagct gctagtatgt
    21541 attcgctatt ttttatttgt ctaacttctg ttctcactat cttagatata ttcatccaac
    21601 ttgttatccc tattactatg gacatagata ctggatttga atttccaagt atagcttgta
    21661 caaatattat aattaatatt gaaggtatac taagtatgat ttctgtaaac ctcatcattg
    21721 catcatctac aagttcagat gctgaaccac ttatactacc ataaacaatt cctataacag
    21781 ttgatattac agtggaaaat agtcctataa ataaagatat ttttcctcca taccatatca
    21841 tagaatatat atctctaccc attgagtctg ttccaaataa aaaagtttta tttggagcta
    21901 aattagaact tgctaaatcc atgtatgttg gttcatgtgt cattataaaa gatgagaata
    21961 cacttccaat gactatgatt gataaaataa taattgatat atatggtaat tctttaattt
    22021 ttttagataa ggttttcttt gtttctatat gttcatcttc tgctactaaa atataatttt
    22081 ctcctacaat ttcaaaatca ctatattgtg gcttcattag tatcacctct atcatatttc
    22141 attctagggt ctattttatt gtttataatt tgagctaaca tattcccaaa tactactaaa
    22201 gcccctgtta ttaagcataa aactaataac atattataat catgatactt tgcactttca
    22261 aagcaaagtg ttcctaaccc aggataagaa aaaacttttt caactacata agttcctcct
    22321 agtatatgag gtatcgaaat ggccataata ctaatatatg atggcattat atttctcagg
    22381 caatgcttaa aaacaatagt tctattattt aaaccttttg ctttacataa aagcacataa
    22441 tcttctctta tttcctctag taatttattt cttatcatat atgtgtaata ccatagatgg
    22501 cttaatataa gtactgtaag aggtaagatt agatgtgata tcctacttaa tatactggac
    22561 tcttctccca ttgaataagc accactactt ggaagtatac ttaagtttat actaaatata
    22621 agtatgagta ctaaggcaac ccaaaatgat ggaatacagt tggttattgt tcctaatttg
    22681 catatgattc tatcaatgag cttatcttca tgtaatgtac aaaatattcc taacaataag
    22741 gctagcacaa aggtaagtat atatcctgaa ccacctaata ttatagtgtt tatatagaca
    22801 tcatttataa cagaagtaac attttgttta tatttaaatg aaattccaaa ttcccctttt
    22861 gatgcatttg aaatccattt tatatattga ctgtatattg gttcatccaa accaagtttt
    22921 ttcatagcat tttctttttc ttgtgtactc attctttcta ctccatcccc atagtaagag
    22981 attaatgggt ctcctggagc aagtcttgcc atgtaaaaaa caataaaaga taataaaaac
    23041 attactaaaa taaactgcaa caactttttc agtatatcct ttaacaaacc ccaacccccc
    23101 tttgttgcaa taaaaaaaac cacataaaag caaacgtaaa gtaacgttaa aaccttcgtg
    23161 gttccattat cattaaacaa aattaaattt tctatattta t
//
LOCUS       DAGSHT010000041        22846 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGSHT010000041 DAGSHT010000000
VERSION     DAGSHT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.042"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            383..1423
                     /locus_tag="MJB72_001450"
     CDS             383..1423
                     /locus_tag="MJB72_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8262569.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1778..3100
                     /locus_tag="MJB72_001451"
     CDS             1778..3100
                     /locus_tag="MJB72_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBY8262570.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3397..4758
                     /locus_tag="MJB72_001452"
     CDS             3397..4758
                     /locus_tag="MJB72_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBY8262571.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4831..6225
                     /locus_tag="MJB72_001453"
     CDS             4831..6225
                     /locus_tag="MJB72_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8262572.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6287..6757
                     /locus_tag="MJB72_001454"
     CDS             6287..6757
                     /locus_tag="MJB72_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBY8262573.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6760..8976
                     /locus_tag="MJB72_001455"
     CDS             6760..8976
                     /locus_tag="MJB72_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY8262574.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSLVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            9059..9730
                     /locus_tag="MJB72_001456"
     CDS             9059..9730
                     /locus_tag="MJB72_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8262575.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            10093..11307
                     /gene="dpaL"
                     /locus_tag="MJB72_001457"
     CDS             10093..11307
                     /gene="dpaL"
                     /locus_tag="MJB72_001457"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBY8262576.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11434..12621
                     /locus_tag="MJB72_001458"
     CDS             11434..12621
                     /locus_tag="MJB72_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBY8262577.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            12754..14124
                     /gene="hydA"
                     /locus_tag="MJB72_001459"
     CDS             12754..14124
                     /gene="hydA"
                     /locus_tag="MJB72_001459"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBY8262578.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGEDLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            14142..15548
                     /locus_tag="MJB72_001460"
     CDS             14142..15548
                     /locus_tag="MJB72_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8262579.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            15620..16066
                     /locus_tag="MJB72_001461"
     CDS             15620..16066
                     /locus_tag="MJB72_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBY8262580.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            16105..16899
                     /locus_tag="MJB72_001462"
     CDS             16105..16899
                     /locus_tag="MJB72_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY8262581.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            16889..19180
                     /locus_tag="MJB72_001463"
     CDS             16889..19180
                     /locus_tag="MJB72_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY8262582.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            19181..20494
                     /locus_tag="MJB72_001464"
     CDS             19181..20494
                     /locus_tag="MJB72_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY8262583.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            20530..21615
                     /locus_tag="MJB72_001465"
     CDS             20530..21615
                     /locus_tag="MJB72_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBY8262584.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            21845..>22846
                     /locus_tag="MJB72_001466"
     CDS             21845..>22846
                     /locus_tag="MJB72_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262585.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEVKQEMIVMKEDMSEVRQEINIMKEDISEVRQ
                     EMTVMKEDMSEVKQEINVLKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEI
                     NIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSV
                     "
BASE COUNT         8916 a         2512 c         4622 g         6796 t
ORIGIN      
        1 atataaaaaa cacaaaaaat gatttaaaaa agataatttt taattataaa ttttaaaaat
       61 aagaatttta aaatttataa aagtaatttt atataggtat aaaaattacg agtagtataa
      121 aaaatttaat aaggatttaa attaaaaatt ttaaaaatta tttccatata aaatatggca
      181 ttgtaattgc ttatttaata gagcattttt gtaaaatgaa gaaagtatat ctatcaaatt
      241 aaaactaagt agatatactt tttattaagt tttgttttaa aataatatta ttaaattata
      301 actaagcttt tctgttatga tataattaaa tagataatgt gaatgattat tatatagaat
      361 acttatttgg agttgatatt ttatggacaa acacagtgat tcagtaatgt atggtggaat
      421 ggaagtatat aatatttggg gaacaacaac agtagactcc ataattggag agtctgatgt
      481 aatgcgtgct cttaagaata gaatttgtaa aattggaaat agtgattcta ctgtagctat
      541 aactggagaa agtggcactg gaaaagaatt aatagcaaga gccattcatc tcgcaggaaa
      601 cagagggaat aaaacattcg ttgctataaa ctgtgctgct ataccagaac ctttactaga
      661 aagtgaactt tttggatata caaaaggagc ttttagtgga gcagattcaa aaggtaaaat
      721 tggtaagttt gaattggcca atggaggggt tgtattttta gatgagatag gagatatgcc
      781 aattcaacta caatcaaagt tattgagggt attacaggaa aagaagttta cgagaatagg
      841 ttcaaatgaa ctaattgata tagatgttag aatcatatca gctactaata gagatttatt
      901 tgagttggtt aaggaaaata aatttagaga agacttatac tatagattga atgtaatacc
      961 tttagaagtg cctccactta gagaaagagg agacgatata caaatattaa taaacaattt
     1021 tatagacaaa tattcgagaa agatgaagaa aaacgtgtac catatagagc cagatgtgga
     1081 acaaatgttg cttaagtatc cttggccagg aaatattaga gagttggaaa atactataga
     1141 actggcatta aaccttcttg aggaagatgg aataatacat aaaggaataa taaatagaaa
     1201 gatacttgaa tattttaagt taaataatat aaatataaag ttacttgagg aaagtgacta
     1261 tgagataagt atggaacaag acatacttac acttgaagag tttgaaagag cctatataaa
     1321 gaaagtgtta aagttttacg gaaatgatac taagtctaaa aaactggctg ccaaaaaact
     1381 tggaatatca ctagctacat tgtacagaaa aatagatgta taataattag gctttttatt
     1441 ctcaattcta aaatttgaaa ttataaaatt gataaaaaac acttggaaaa ctattttcta
     1501 agtgtttttt tgtgtaatag cattttatta tccaaaaata aaaaaaattt aaaagaattt
     1561 aaaaaaataa atgctttaat aaattatcaa atactaacaa atataataaa aattatatat
     1621 gtaaaacaat tatgttaaaa aattggcatt ataattgctt catattaagt tgagatgaca
     1681 aaataaaaaa agaaaggatg ttgtgtgaga taataataac tgtcattaaa aatagttggg
     1741 agtgataacc ttgagtaaat atcaaaagaa tgtttctatg tttgatattg atggaaggcc
     1801 accaatttta aagtgtttgc cactatcaat gcaacatata ctagcaatga tagttggaac
     1861 tgttacagta cctatagtcg taggaggagc tgtgggtgtt agccctacac aaataacact
     1921 tttagtgcaa tatgcattaa taatagctgg tatatcaaca cttatacaac tatatccaat
     1981 aggaaatatt ggttcaagat tgcctataat ttttggagtt ggttttactt atgtaccaac
     2041 acttgtagca gtcggaggaa attatggatt agcaagcatt ttgggtgcac aattaatagg
     2101 gggagtggct acagtattaa tagggatatt cataaaaaag ataagaaaat tttttccgaa
     2161 tatagttgca ggaacagtgg tttttacaat tggattatct ttgtatccta tagcaataaa
     2221 ttatatggca ggtggtgtaa atagtcctac ttatgcctct atgcaaaact gggcagtagc
     2281 ggctataaca ttagtggtag ttataggtct tagtcaattt gcaaaaggat atgcaaaatt
     2341 ggcagcaata tttttgggaa ttatagttgg atattgtgta tctttagttc tgggtataat
     2401 aaattttgac cctattagag aggcaacttg gtttgcgata cctaaaccgc ttgaatttgg
     2461 gatggaattt gatatgcctg taataatctc agtagtaatt gtaagtataa tcaatgctgt
     2521 tcaagcaatt ggagacttat cagctactac aatgggaggc atggatagga atatcacaga
     2581 taaggaatta tcaagtggag taataggaag tggaatttgt actatgatag gagctttgtt
     2641 tggaggttta ccaccatcat catatagcca aaacgtgggg atggtagcta tgaacaaagt
     2701 aataagtaga tttgtgcttg gaatagctgg tatatttatg ttattgtcgg ggtttgtgcc
     2761 taaatttggt gcattaatga ctactatacc atattctgtt ttaggaggag ctacaatatc
     2821 tatatttagt atgataacaa tgacaggtat aaagttaatt attacagaag agttgtcatc
     2881 aagaaatatt accattgttg gtttggcaat tgccttaggc atgggaataa catcagtccc
     2941 agcagcccaa gaggcatttc cttcatttgt aaagttagta tttggagaat ctccaatagt
     3001 aattgcaaca ttggttgcat tcgttttaaa tctaataata ccaaataaaa gtctagagga
     3061 tgaggctaaa gaaagagctg ttatggaaga gactaattaa gatataataa tttaaaagaa
     3121 ttgtttaaat tatttagtat gagattgtta gattattttt gatagctatt cattttaata
     3181 aaaaatttaa caaataaata tataccaggc aattaatgtt catttaagtt gaaaatcggg
     3241 gaggcaatat ttagaaattt gttaattttt tataaaaata gctcagttta gctgtgttta
     3301 tcggttgagc aataaattca tggtcataaa aatatttata gaaaataatt ataaaaatat
     3361 ttagtcaaga tatttatgta tgggaggatt tattttatga aaacacgtaa tacttcaaaa
     3421 tatgatgtcg atgggatacc accactaaag gaatcaatac ctttggcctt acaacatctg
     3481 ttagcaatga tagtaggaaa tatggtacct gcgatattaa tagcaaacgt ggtaggactt
     3541 aaccaggggc aagcaacaat gttgattcag ggttctatgt tagcagcagg tttagcaacg
     3601 tttttacagt tatatccaat acctttattt aaaggattta agttaggttc aagattacct
     3661 gtaatgatgg gaatgagtta tgtattttta ggagcttgtc tttcagtagc agctaaagat
     3721 ggtttagcag ctctatttgg agcacagatt gctgctgggg taatcgtatt ctttgtagga
     3781 tttggagtta agaaaattag acatatattc actccaatag tttctggaac tataatagca
     3841 tgtatgggac ttggactatt cgcaacagct ataaagaatt tagctggagg agaaggcaca
     3901 caaacatttg gaagcccaat aaacttcaca gtaggagtta tagttgcttt tgtaataata
     3961 atgataaata aatacggtaa aggtttagta aaaaattctt caatattaat aggaatatta
     4021 gtaggttatg ctatatcttt agttttagga ttggttgatt tctcagccgt tcaaggagca
     4081 gctatagtat ctttaccaac accagcagct tttggattag aatttagacc agagttaata
     4141 gtaatgttta ctattattta cataataggt atagctgata tgatgggtgc ctgtacaata
     4201 gctacagtag gagcaatgga tagagaggta actgacgaag aattagcatc agttgtatta
     4261 ggaaattcta taacttctat aatttcatca ctatttgcag ctcttccaac tggagtattt
     4321 agccaaaata cagtaattgt atctatgaac aaagttacaa gtagatttgt aatagcacta
     4381 ggggcacttg tattattact agcagggata tctccagcat tgggtgcaat aatgacaact
     4441 ataccatctt gtgttgttgg tggagcaact ttagtagttt tctcatcaat agcaatgtct
     4501 ggtttctcta ttatgagtat ggatggattc actgaagaaa acaatcttat agcaggtgta
     4561 tctatagcaa caagtatggg tcttacaact gctccacaag ttttagacca attcccagaa
     4621 acaattggaa ctgtccttgg agggtcaagt atagtttctg gagcaataat agctatatta
     4681 cttcaaacat tatttaaatt aaagtcacgt aaaagtgctg aaaacgttac ttcaaatctt
     4741 gaggaaaata ttggataaat gtattagata taatatgaga aaaccatatg gtaaaatgtt
     4801 gtaatgaaag tattcaaagg gggatttaag atgattgata tactaataaa aaatgcaata
     4861 attgttacag ttaataaaga cagagatgtt atatttgatg gagcaatagc tattcaaggt
     4921 gacagaatct tggacatagg aaatacagtt gatattgagc ctaagtatct tgatgctaaa
     4981 aaagttatag atgctaatgg aaaagtaata ttccctggct ttataaatac tcataatcac
     5041 ctgttccaag tgttattaaa aggtttaggt gatgatatgg cacttcatga gtggttaaat
     5101 acaatgatgt ttccatcagc aaaattctta acagaacaag atacttatga tgctgcaatg
     5161 cttggatgta tggaaggttt aaaaagtggt ataactacaa tggttgatta tatgcatact
     5221 cacaatagac caggacttac agatggaata gtaaaggcat ataaagattt aggtataaga
     5281 ggggttgtag gtagaggttg tatagattta ggtattcaca aagagcttat agaagatgtg
     5341 gaaacggttg aaaaagattt gagaagggta ttcgaaaaat atcataattc agaaaatgga
     5401 agaataaaaa tctgcgtagc tccttcttca atgtgggcta tatctgaaga aatgggtaag
     5461 atgctttgga atatagttaa agaatatgat tcatatttta cagttcatat atctgaaaca
     5521 gaatttgcaa gaacagcaac aaaggatata catggagaaa tagatataaa attacttgaa
     5581 aaatggggta tagtaggtcc agaagttgta gcagtacatt gtgtatgtat aactgatgaa
     5641 gacatggaaa tgcttaaaaa gtatgacata aaagtttctc ataatgtagc aagtaacatg
     5701 tatcttgctt ctggtgttgc acctgtgcca gaaatgttaa aaaaaggtat aaatgtgagt
     5761 ttaggtcttg acggtgcagc aagtaataac gcacaagata tggtagagct tatgaaatta
     5821 acagctttac aacacaaagt aaataattgt gaccctctgg caatttctgc agagaaagtt
     5881 cttgaaatgg ctacaattga gggagcaaga actcttagaa tggaagatga aataggttct
     5941 ttggagattg ggaaaaaagc tgacctagta atatttaact caatgctttc tccaaaatca
     6001 atacctcttc acaatcctgt atcaactttg gtatactcag ctagtatgca caatgtagag
     6061 agtgttttag ttgatggaaa tgtaatactt gaaaatggaa aagtaactac aattgaaagt
     6121 gaagaacaag tgttgcttaa tgcacaacat gcagcagaag aattatgtaa gagagctaat
     6181 gtaactaata gaatggaagg acataagtgg aatagcttat actaatatta aaataagatt
     6241 gtaataatat tctattacaa cattagttta tctgtaggag gagaaaatgg aaaaaataaa
     6301 attaaatatt aatgattaca tgtatgaaat agaagttgaa aagaatgaaa ctcttttgca
     6361 tgtacttcgt gaaaaattaa atctaactgg agcgaaacaa ggttgtaata gtggcagttg
     6421 tggagcatgt aaagttttga tagatggagt tgacatgaaa tcttgtaaac ttcttgcaaa
     6481 aaactgcgaa ggtaaagtta tatacactat agaaagtttt gctaatggag aaaatcttca
     6541 tccaatacaa gaagctttta tagaagtcgg agctgtacaa tgtggatatt gtactcctgg
     6601 tatgattata acaacacaag ctcttttaaa tagaaatgca tctcctaatg aagatgaaat
     6661 tagagaagct tttaaagaaa atctctgtag atgcacagga tatgtaaaga ttatagaagc
     6721 tgtaaaatta tctgcagcta gattaggggg tagataatta tggaaaaggt aataggaaaa
     6781 tcataccctc taaaagatgc agctttaaaa gttactggtc aaatgaaata cgtggctgat
     6841 atgaaaccac aaaatgtact ttacgctaag atgcttttaa gccttgtagc acatgcaaaa
     6901 ataaaaagta tagatacaag tgaagctgag aaagtagtag gtgttagagc tatagctact
     6961 tatctcaata catcacaaag aaaatacaat ggttcattaa gattttatga gcacaaaata
     7021 cctgaaaatg aagttatatt ttcagataca gttagatttg ttggtgaccg tgttgcagca
     7081 gtagctgcag agactccaga aatagcagag aaagctgtaa aacttataaa agttgaatat
     7141 gaagaattgc cagctgtatt tactatacaa gatgcaataa aagaagatgc tatctgcata
     7201 catggagatt caaatatagt ttctcaaaac aacataaatg ggggagatgt agaaaaaggc
     7261 ttcaaggaat gtgattatat atttgaagat gaatactcta ctccagcaat acatcatagt
     7321 gcaatagaga ctcatagtgt aattgcacac tatgattata atgataaatt aactgtacat
     7381 acaccttgcc aaaatacatt tggatttaga ataatactat ctcaaatttt tgggatgtca
     7441 ttaaataaag taagagttat aagacctgct attggaggct catttggtgg aaaattagaa
     7501 cttacaattg aaccagtagc agccgcatta tcaaaaatga caagaagacc agttaaaatg
     7561 gttcttacaa gaaaggaatc tatgatatca acaagaactc gtcatgggtc ttttgtaaaa
     7621 ttaaaaactg gtgtaaataa agatggtgaa gtaatagcac aagacataaa aatatataca
     7681 aatacaggag catatgcatc aagtgcttta aatgttatag gagcacttag tcataaagta
     7741 tttaaagttt ataaaatacc aaatataaag tttactggga tgcctgtata tacaaataca
     7801 ccaatagcag gagctatgag aggctatggg tcacctcaaa tatttatggc tcaacaagct
     7861 caatttgcga aaatagcaaa ggaaataggt atggatttag tcgattttca aaataaaaat
     7921 gcagtagagc cagatgatgt cgatataatc ttccacggtt ctcttggaaa tcctagagta
     7981 ttggattgta tagagcaagg taagaagatg tttaagtggg atgaaaagaa aaaacttcca
     8041 aaagaagaag gaaaatattt aagaggtata ggtatggcaa taggtgcaca tggtaatgga
     8101 gtttttggtg cacatagaga tgttgttaca ttgactctta aattaaatga agatggtacg
     8161 ttgacactac taacaggagc tcatgatatg ggtaatggtg ttgtaactat gcaaacaatg
     8221 atggtagctg aagttttagg aataacacct gataaagttg atacatttga aactgatacg
     8281 gatgcatgta cattcaacct tggtgactat gcaagtagag gggtatttgt agaaggtgga
     8341 ggagctaaaa agacagcaga gaaattgaaa aatcttatat tagaagaagc agaaaaatta
     8401 cttgaaacat caaaagaaga tttataccta gacaatggat gtgtaattag taaacttgat
     8461 gaaaatgtaa aagcatcact ttctgatgtc gcagtatata gtcaaagtaa atcattaaga
     8521 gagttaactg ttgttgaaga ttattcttca ccagctggac gtacttctta tggtgtacat
     8581 tttgctgaag ttttagtaga taaagaaact aaggatataa aagtagttga ttttgtagct
     8641 gtacatgatg ttggtagagt tataaatcct ctaaatttag aaggacaatt agaaggtggg
     8701 attcatatgg gtctaggata cgcattgagt gaagaattgg catttgatga acaaggtaga
     8761 cctaaagcta ctgattttaa aacatataaa ttatttagaa ctgtagatat gccaaaatgt
     8821 caagttgcat ttgtagatgg ttatgaaaaa gctgggcctt ttggtggtaa gagtattgga
     8881 gaatgtgccg ttgtaccagt tgcgccagct gttgcaaatg cagtatataa tgcaactgat
     8941 gtagagatac attcattacc aataaaatta aaatagtaga aattaaagca gaaataacaa
     9001 tttattgtat ttgtaaatag ataataaaaa tagatttatt tggtgaggag atattattat
     9061 ggcaaagata ttaggtattt gtggaagtcc acgtaaagga gcaacagaat atgcagtgtt
     9121 agaagctttg aaagaagcag aaactatacc tggtatagaa actgagtatt ggtcagttag
     9181 agggaaaaaa ataagtcctt gtgtacattg tgatgcatgt ataagaaaag aaactatgtg
     9241 tattataaaa gatgatatcc aagaattaga gcaaaaaata ttggaggcag atggaattat
     9301 agtaggttct ccagtttatg atatgaatat aactgctcaa cttacagcag tatttaatag
     9361 acttcgtcca atgtatttag ttcatccagg aaaacttcaa aataaggttg gtgcagctat
     9421 aactactggt ggaactagat atggtggcca agaattgaca aaacttccta taataaattt
     9481 cttcctaatg catgaaatgt tagtgtctgg aggtttaggt ggatgctata taggtggtac
     9541 aatatggagt aaagatggaa aagcaaaagg tgcccaagag gatgagacag gtatggacac
     9601 agtaaaaaga cttggaaaag gtgcagcaga ggcagttatg gtttctaagt ttggtcttga
     9661 aaaatggaat gtcgtaaaag aagaattaga tgttaaaaaa gatgaaaaat cacctcttag
     9721 agaccactaa agatttatta tattattgaa tatatacaaa tagtgatgta atagattatt
     9781 gcatcactat tttatgtttt aatttagatt ttataaattt tatattaaaa aatatagaaa
     9841 caaagtgttt tgagatttat ttttaaattt taaaaatgag aattaaaaag tgagaagata
     9901 gaaaaggttt tttagttatt aaattctaca tacattgtat gaactaaaga tagaaaagaa
     9961 aaaataataa aagcattgca atttctatac taaagagtca tggaaaaata tctagtatat
    10021 atttagtatt ggcatgttaa ttgcttatat attaaagtat aaaaatttaa caataatttg
    10081 gaggtatttt agatgttaaa agaaattaaa tggaaagtaa ataatttgcc aaagggcgat
    10141 aaggaaaatt gtatcaaatt tttaaatgaa gaagaaataa ctaaggttag aaattttcac
    10201 aaaagctttc ctcaatataa agaaactcca ttagcaaacc tagaaggttt agcaaaaaaa
    10261 ttaggtgttg caggagtata tgtaaaagat gaatcttata gatttggatt aaatgcattt
    10321 aaagtattag gtggttctta ttctatgggt agatacttag ctcaaagatt agatacagat
    10381 atatctgagt tagggtatga caaattgact tcgaaagaaa taaaagaaaa attaggagaa
    10441 ataactttct ttacagctac agatggaaat cacggtagag gagttgcttg gacagctaat
    10501 aaattaggtc aaaaatctgt tgtattaatg ccaaaaggtt cttctgaatt tagattaaat
    10561 aaaattaaag gagaaggtgc agatgctagt ataacagact taaattatga tgatgcagta
    10621 agattagcta atgattatgc agaagctgat gaccatggag taatggttca agatacagca
    10681 tgggacggat atgaagaaat accagcatgg ataatgcaag gatatggaac tatggctcaa
    10741 gaagctatag agcaattaaa agaatatgga gtagatagac caacacacgt atttgttcaa
    10801 gcaggagttg gttcacttgc tggtgcagtt caagggtatg ttgcttcaat atatgatgaa
    10861 tgcccaataa cagtagtagt agaggctgat gaagctgatt gttactataa atcagcagaa
    10921 gctggagatg gaaaaccaag atttgttgga ggagatatgc caactataat ggcagggctt
    10981 gcttgtggag aaccaaatac tataggattt gaagtattaa aaaatcatgc agcagcattt
    11041 gtttcagcac cagactgggt ttctgctaaa ggtatgagaa cattaggtaa tcctttaaat
    11101 ggtgatgaaa aggtaatttc tggagaatct ggagcagtta caactggtct tttagtagca
    11161 gctatggaaa gagaagattt agcagattta agaaaagatt taaaattaga tgaaaattca
    11221 agaatacttt taataagcac agaaggagat actgaccctg acaaatatag aagtatagtt
    11281 tgggatggag aatatccaag tatataagga attactaaaa tgcaattatt agatattggt
    11341 ttacatattt agaagatata taagttctaa aatttagaaa atatataagt atataaaata
    11401 catacattat aaaaaatcta gggggataat gaaatgttaa acgaacaaag aaaacaagaa
    11461 gtaactgaaa tatgccaaaa attaattcaa aatcctagtt cttctggaaa tgaagaaggt
    11521 gttgtaaaag caatagaaga gtcttttgaa aaattaggtt ttgatagttg gtcaagagat
    11581 agatatggaa atatagtagg atgtataaaa ggtaacaagc caggtaagaa aatattattt
    11641 gatggacata tagatacagt tccagttcca gatgcaagta aatggagtgt tcctcctttt
    11701 gaaggaaaaa tagttgatgg aaagatatat ggtagaggaa cttctgacat gaaaggtcaa
    11761 tatactgcaa tgatgtctgc tgtagcgtat tttgctaaag acactaatag agattttgct
    11821 ggagaactat atgtagcagg tgttgttcat gaagaaatat ttgaaggtgt ttcggctaga
    11881 gagataagta aggctgttca acctgactat gttgtaatag gtgagtcttc agagttaaac
    11941 ttaaagattg gtcagagagg tagaggagaa atagtagtag aaacttttgg taaaccagct
    12001 cactctgcta atccagaaaa aggtgtaaat gcagtttata aaatggctaa tgtaatccaa
    12061 aagatacaac aattagtacc tccaactcat ccagttttag gtgatggaat attagtttta
    12121 acagatataa aatcttctcc atatccagga gcatcagtag tgccagatta ttgcaaagct
    12181 acttttgata gaagattatt agttggagaa acaagagaag gcgtattagc tccaatacaa
    12241 gctttattag atgaaatgat gaaagaagac ccagaattaa atgcaaaagt ttcttatgcg
    12301 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
    12361 tggttatatg acgaagaaga tgaatttgtt caagcagcat ataagggatt aaaagaagct
    12421 ggaatagatt cagaaataac tcaatactca ttttgtacga atggaagtca ctatgcaggt
    12481 gaagctggaa taaaaactat aggatttggt ccttctaagg aaaatcttgc tcacacaata
    12541 gatgagtata tagagcaaga acagttattt ataggaacag aaggttacta tggaatatta
    12601 aaatctgtat acggaaaata atatttagtt cacaataaat tatttaaata tagtctagag
    12661 gtgctgaata tagcaccttt agaaaataca attttaaata aagttttaca atacttagaa
    12721 aatactttaa agcttaaaag ttaggaggta tatatgggaa caatactaag aggtggaact
    12781 ataattacct ctgataagac ttatataagt gatttaagga tagaagatga aaagatagtt
    12841 gaaataggaa ataacttaga aataaatggt gataaggtta ttgatgctac aggtaaaatt
    12901 gtaattcctg gtggaataga cacacatact cattttgata tgaatgcagg ttctattaca
    12961 actgcagata attttgaaac aggaacaaga gcagctatag ctggaggaac tactacaata
    13021 ctagattttg cagaagccaa tgagggggaa gacctactgc aaggtgtaga agcatatcat
    13081 aaaaaagcaa gtggaaattg ttattgtgat tatggttttc atatgacaat tacatgttta
    13141 aataaagata catttaatca tatggaaaaa cttatttcgg atgggatagt atcttttaag
    13201 atgtatatgg catatgatgg catgaaagta gatgatggaa caatatacag ggttttaaag
    13261 aaagcaaggg atttaggttg tatagttgga tttcattgtg aaaatgggga tttactagat
    13321 gtattgatag aagaaaatat acaaaatggt aatttagagc caaaatatca tccacttaca
    13381 agacctaaca tagttgagaa agagtctgtt tcaagacttg ctgatataac taaattatct
    13441 aattcaaaat cctatgtagt ccacttaagt tgtagggagt cacttgaaac tgtaaaaatg
    13501 gctagagaaa aaaatataga tatgatagta gaaacatgcc cacagtatct attattagaa
    13561 gacaatttat ataataagga taaatttgag ggtgctaagt atgtaatgtc tcctccttta
    13621 agaaaaaaag aagacataaa ttacctttgg gaaggattgt ctagtggaga tattcaaaca
    13681 gttggcacag accactgttc atttaatttt aaggggcaaa aagacttggg aattgatgat
    13741 tttagtaaga ttccaaatgg agcacctggt gttgaacata ggttagcact tttatataca
    13801 tatggggtct tagaaaatag aataagtgta aataagtttg tggaagttac ttctacgaat
    13861 gctgcaaaaa tatttggaat gtatcctaaa aaaggtgaga tagctgtagg aagtgatgca
    13921 gatatagtca ttttaaatat caataaagaa gaaacaatta gttataaaac tcaaaaacaa
    13981 aatgtagatt atactccata tgaaggattt aaggttaaat gtaaagttga agatgtattt
    14041 ttgagaggaa atcatgtagt acaaagttgt aatgttaaag aacatccaac aggtcaatat
    14101 attaaaagaa aaattactaa ataatgcggg gggaaataag aatgatggat atacttatta
    14161 agaatgccat aatagtaact gtgaataaag agagagaagt tatttttgat ggggcattag
    14221 tagtaaaaga taacaagatt gctgatattg gaaattctaa agagatagaa agtaaatata
    14281 ctgatgttaa aaaaattata gatgctaaag gtaaagtttt attcccaggt tttataaata
    14341 cacataatca tttattccaa actttattaa aaggattggg agatgatatg gtacttaaag
    14401 actggttaga aactatgaca tttccagcag ctaactattt agagcccaaa gatacttatg
    14461 atgctgcaat gcttggatgt attgaaggac ttagaagtgg tataacaaca atggttgact
    14521 acatgtatcc tcatagtaag ccaggtcttt gtgatggaat aatagatgct tataaagaat
    14581 taggaataag aggaatactt ggaagaggat gtatgaatac aggagctcaa tttggagttc
    14641 atcctggaat aatgcaagat gtagaaacgg ttgaaaaaga tgtaagaaga ttgtttgaaa
    14701 aacaccataa tacagaaaat ggaagaatca aaataggagt agctcctgca gcaatatggt
    14761 caaattctca agaaatgctt gaaatgctat ggagagtagt aaaagaatat gatgatgctt
    14821 tatttacagt acatatatca gagactcctt ttgatagaga agcagcaaaa gaattgcatg
    14881 ggcaatatga catagatgtt ttagaaaaat taggaatact tggaccaaat gtgcttatgg
    14941 tacattgtgt gtatctaact gaaaaagata tggaacttac taaaaaatac gatatgaaag
    15001 tgtctcataa tacagcaagt aatatgtact tatcttcagg tgttgcacca gtgccagaaa
    15061 tgcttaaaaa aggaataaca gttagtttag gagtagatgg agccgctagt aataactctc
    15121 aagatatgct tgaacttatg aaattaacag cacttcaaca taaagttaat aagtgtgacc
    15181 cattagcaat gtcagcagaa aaagttcttg agttggcaac tatagatgga gcaagagcta
    15241 ttggaatgga agatgagata ggttcattgg agataggtaa aaaagcagac ttactaatat
    15301 ttaatccaat gctttcacca aaagcgatac ctatgcataa tccagtatct acattagtat
    15361 attcttctag tatgaaaaat atagagagtg ttatagtaga tggaaatata ataatggaag
    15421 attcaaaaat attgacagct aatgaagaga aagcattaaa agatgctcaa gatacagctg
    15481 agagactttg tgtaagagga actataaaaa atagaatgga aggtcataaa tggaatagcc
    15541 tttattaata aatgtaattt attcatagaa aagtatagta aaactttagc ttaaaaatat
    15601 gagttttgag gtgatttatt tgattatcaa atgtaccata aataataaag aaaaagaatt
    15661 aagtataaga ccagatgatt ttttagcaga tacattaaga gaaaatggat atgtaagtgt
    15721 taaaaaaggg tgtgatacag gctcatgtgg gttatgtaca gtgatggtaa atgacaaggc
    15781 cgtgctttca tgtaattatc ttactgctag agtagacggt cagcatatta caacacttga
    15841 gggtgtcttg gatgaagcaa agaagtttgg agattttctt gcagacgaag gggctgacca
    15901 gtgtggattt tgtgctcctg gatttataat gatggttttg gcaatgaaaa gagagcttca
    15961 aaatccaaca gaagaagaag ttatagatta tgtaaatggt aacctttgta gatgtacagg
    16021 atataaaggt caattaagag caatacataa atatttaaac acttagactt ttaagtgaaa
    16081 attaagttga tatggggaga gagtatgata actataaagg aatatgtagt tccaaaaagt
    16141 ttagatgaag cttatgaact gctaatatca agaaaaaaca acataatact tggaggatgt
    16201 ggatttttaa aactaggtag taaaaatatt ggatcagcaa tagatttaaa agacttagct
    16261 ttagattata taaatgaaac tgatgatagc atattaattg gagcagacac aagtttaaga
    16321 actttagaat taaataaggt tattaaaaat tactgtaatg gggtaatttc aaacgctgtt
    16381 tctaatatag taggagttca atttagaagt ggagctagag taggagctag cgtatttgct
    16441 aagtatggat tttcagattt aataccttca ttactagtgg tagatgcaaa ggttaagtta
    16501 tataaaaaag gggttatgaa cttagctgat tttttagaaa gtgagcttga aaaagatata
    16561 ttaatagaag ttatattacc taaaaaagat gctataggtg tatttgattc tataagaaaa
    16621 tgtacaggtg attttgcagt attaaatgga gcaatgttaa aagaaggaag tatttataaa
    16681 attgcaatag gcgctagacc aagaagagca aaaattgcct ataaagcaag tgaaatatta
    16741 agtttagaaa atgatataga aaaagctggt gaagtatcaa gtgaagaact ttcatttgga
    16801 agtaatataa gaggaagtaa agaatacaga aaagatatgg caaaagcctt agtagtgaga
    16861 atgtataata gtattggagg tgagtgtgat ggtaaataaa gaggtaagaa aaatagatag
    16921 tgaagccatt gttactggaa aaccaatata tacagaagat ttaatttttc ataaagatgt
    16981 tttaacaatt aagcttctta gaagtccaca tgcttttgcg aagataaaaa acatagatat
    17041 aaaaaatgca ttaaaggtaa aaggtgtagt aaacatattc acttacaaag atgttccaaa
    17101 ttatagatat tcaatggttg gagagtcata tccagaagca tcacctcatg accaattaat
    17161 acttgaagac gtagttagat atgtaggaga tgaagttgct ataatagccg ctgaagatga
    17221 aagggctgct attaaagcta tgaaacttat aaaagttgaa tatgaagtaa tgactcctat
    17281 attggatgca agagaatcgg aaggaaataa aattttaatc catgaagaag atgatttgtt
    17341 ttgtccattt gattttggat tggatgctac aaaaaatatt gtatcgagag aaaaaatggg
    17401 taaaggggat gtagaaaaag agtttaaaga aagtgatgta atagtagaac attcttatat
    17461 gactcaatca caagcccatt gtatgatgga aactcataga gcttatacgt atatagatac
    17521 gaatggcaga ttagtagtaa cttctgcaaa ccaatcagct tatcatatga gaagacaggt
    17581 ttctaaagca ctgggaatac ctctttcaaa agtgagagta ataaaaccaa gaataggtgg
    17641 aggttttggt ggaaaaaaca tagctgtaac agagatatat gcatcatttg taacttggat
    17701 gacaaaaaga ccagcaaaac taatttatac tagagaagaa acttttgcaa tgactaatac
    17761 tagacatcag atgttttttg atataaaaat tggggcagat aaagaaggaa acataaaagc
    17821 tatagatatg aaagcattga acaatacagg cgcttatggt gataatggac cttcagtttg
    17881 ctctgaatca ggtcataatg tacttccaac atataataat gtacctgcaa taaaatttga
    17941 tgggagaact gtatatacta atttagttcc aggaggagca ttaagaggat atggagcaac
    18001 acaaggtaca tttgcacttg attgtgcaat tgatgaatta gcacataaac ttaatatgga
    18061 cccaacagaa ataaagttaa agaatattat aaaaaaacaa actgaaggcg gaataatgaa
    18121 ttttccaata agaagttgtg cacttgatga atgtataatt agaggtaaaa aacttattgg
    18181 atgggatgaa aagtaccctg ttagggatat tggaaacaat aaggtgaggg ctgttggaat
    18241 gtctgtggga actcatggtt ctggtatagc caatatagac atggcaatag taaatatgag
    18301 gatggatgaa gatggaactt ataagttgtt ttctgggtct tcagacttag gaactggttc
    18361 tgacacaata cttgcacaaa tagctgcaaa agcattgaat acatctattg atagaatttc
    18421 cgtttataca ggagatacag atatgtgtcc atatgatact ggtgcatacg cttcttgtac
    18481 tacttatgta actggaaatg ctacaataaa atctgctgaa agtttaaaac aaaagatatt
    18541 aaaagtagca cagaagaaac tcgatttacc aatagatgat ttaatactat atgaagacag
    18601 agtttgttat agtgaagatg tagagaagtt tgttttgtta agtgaattag gaaaagaatc
    18661 tgttggtggt ggaaatcaag aagttttata ttcatctgaa tcttatggta tacaagagag
    18721 tgctaaacca ttcttagcag gatttgctga aatagagtta gataaaacaa caggggagtt
    18781 taaagtaata aattatgctt gttctacaga ttgtggtacc gttatgaatc ctgcacttgc
    18841 tagaatacaa gttgaaggtg gtgtaactca aggtataggg cttgcaatgt atgaagaccc
    18901 tagacatggt tctgatggaa agcttcaaac ttctaatcta ttacaataca aagttccaac
    18961 aaaaaaagat ataggaaaca taattgttga ttttgtagat aattatgagc caacaggtcc
    19021 atatggagct aaatctttag gagagatagt tattcataca cctgcaccag caatagcaaa
    19081 tgcaatatac aatgccacag gagttcgtat aagagaactt cctataactt ctgagaaagt
    19141 ttatatggcc atgaaaaaat taaatcaggg aggtaaataa atgattgcta ttaaaagtaa
    19201 atacttaata tcttctgctg atgaaatata tacaaattct gctgttgtag tagaaaatga
    19261 cattataaaa gatatattgc caaatgaaga agttgaaata aaatataaaa atattgaaga
    19321 aataatagat aaatcagatg ctataataat gccaggattc gtcaatggtc atatgcatca
    19381 atatggagtt ctttctaggg gaataccggc aaatgtacat tttactgact ttgaaggttt
    19441 tttaaatgac tattggtggc catttataga aaatagaatt ggattaaaag aagtgaaggc
    19501 aacaactaaa gcatcagcaa tagaacttat agaatctggt gtggtagctt tttgtgatac
    19561 cctagaagct ccaaatacag aagaaggaac tttaattgag caaggaaaaa tactagaaga
    19621 aataggcatg aaagcgattt tatccttaga aagttgtgaa agaataagct ttgaaaatgg
    19681 acttagatgt ttagatgaaa attctaattt aataaagtgg agtagagaaa atagtaaatt
    19741 aataaatgga ataatgtgta cacatacaag cttcacatgt tctgatagat ttattaagaa
    19801 agctaaagaa gatgcaaaaa aattaaatgc accaatgcaa tttcatttat gtgagagtat
    19861 atatgagcca aactatgctg aaaaacactt tggcaaaaaa gcagttgact actataatga
    19921 tttagatata ttggatgaaa ctgtattagc atctcagtgt gtaaaagtaa atgatgaaga
    19981 aatagatata ttaaaagaaa aaggtgtaaa agtggttcat atgcctctta gcaactgtga
    20041 agtaggtgga ggtttttctc cagtaccaaa aatgattaaa aaaggcataa aagtggcact
    20101 gggtactgat ggatatataa atgatttttt tgaagttatg agaggtgcat ttttaatgca
    20161 taaatcagtt gaagaagatg catcagtaat gccagcaaat ttagtattta gaatggcaac
    20221 agagcatggt gcttatgtat taggacttca aaattctggt aaaatagcag ttggtaataa
    20281 ggctgatata atagttatgg aagatgaatt taaaactcct gtgactcttg ataatatatt
    20341 tgaccagata gtagttcagg gtaaaaaaga atttatatca aatgtataca tagatggtag
    20401 acatatttta aaagaaaaac agttagttga tttagataaa aaagctattg ttaaagaaat
    20461 gaaagaagtt gcttgtgaat tttggaaatt ttaacgaatg taaatatata tgaaaagggg
    20521 aagcagataa tgtataattt tttaggtaaa gaattaaaat caccacttat aataggttca
    20581 ggaccattaa catatagtgc agctggatgc aagatattaa gtgatgcagg agcaggggca
    20641 gttgtgacta agactataag aaaagaaaga gctataaatc cagcaccaca tatggttaga
    20701 aatacagcta atgctctttt aaataatgaa aaatggacag attttgagcc agagcaatgg
    20761 atagattttg aaataccaca aatgaaaaga gatgggactg tctgtatagc ttcagtaggt
    20821 catacaattg aagaaagtag cgaattagta gagaaagttg caaatgcagg agctgacttt
    20881 attgaattag tatcttatga ctatagagat ttgataccaa tgcttaaaga tgcaaaggaa
    20941 agagtaaata tacctgtaat agttaaactt cctcctatga tagatgagat aggcgatttt
    21001 gctaaaaaat tagaagaagc aggagcagat gcaataactg cttgtgactc tgtaggacca
    21061 gcatttagaa tagacataga aacagggcaa cctttattag gtggaaatgg aataggatat
    21121 ttaagtggag aaactatcaa gccaataact ttacagagaa tatatgaaat aaaaaaacaa
    21181 gttaatatac caataattgg cttaggtgga tgtgtaagtg gagatgatgc cttagaaatg
    21241 attatggcag gtgctgactt tgttggtata tgttcagtag taattttaaa aggcgcacaa
    21301 gtaatatcta aaatacatga tgatttaaaa tctaatctta atagattagg atataatact
    21361 atagaaaatg cttgtggtat agtacataat catatgggag atgttaatga aagacttagc
    21421 tttgagttta cttatgatga agaaaaatgt actaaatgta agatgtgcga gagagtttgt
    21481 gcatatcaag cgagaaaatt gaatgataag atggaattga atgaagaaga gtgtagatat
    21541 tgtggacttt gtatatcagt ttgtaaacca aaagcacttg gaagaaaaat ttcatgttca
    21601 gagctgaatg tatagattat tttataaaaa acaaagtttt aaataaaaat aagaaaggct
    21661 atctcaaaga gataagcctt ttttgttgtt gaccttattt ttgtttattt gtagttattt
    21721 tagatattaa ttagttttga agtaataaag tgaatcaatt ttgtttaatt aaaattttaa
    21781 taattctgat atatatgtta acattgtata gcatttgtgt aaaatgttat aataattaat
    21841 agggatggtg atgcaagtgc aagagaagat tttagagtta ttatatacta taaatgaaga
    21901 tgtagttagc ataaaaaatg atatgtcgga agtaaaacaa gaaataaatg tgttaaaaga
    21961 agatatgtcg gaagtaaaac aagaaatgac tgtaatgaaa gaagatatgt cggaagtaaa
    22021 acaggaaata aatgtattaa aagaagatat gtcggaagta aaacaagaaa tgattgtaat
    22081 gaaagaagat atgtcagagg taagacaaga gataaatata atgaaagagg atatatccga
    22141 agtaagacaa gaaatgactg taatgaaaga agatatgtcg gaagtaaaac aggagataaa
    22201 tgtattaaaa gaagatatgt cggaagtaaa acaggaaatg actgtaatga aagaagatat
    22261 gtcagaggta agacaagaga taaatataat gaaagaggat atatccgaag taagacaaga
    22321 gataaatata atgaaagaag atatgtctga agtaagacaa gaaatgaccg taatgaaaga
    22381 agatacatca gaggtaaaac aagagataaa tgtaatgaaa aaagacatgt cagaagtgag
    22441 acaagaaata actgtaatga aagaagatgt ttccgaagtt aaatcagaag taaactttat
    22501 gcaaaataaa ataaataaca ttaatgaaga tatgaatgga attaaggacg aggtatccat
    22561 agcaaatgaa aaattatatg gaattgaaat taagattgat agtctagaat gtgaagataa
    22621 atcaatgaaa gagatacaga tagaacagaa taatatttta ggaagtttat tacataattc
    22681 tgaaataaat aaagctactc atgataatat agaacataac atagcatata taaaaggtga
    22741 cactaattct ataaaagagg atatagctga aataagaaga gacttaaatt tagtagagtt
    22801 agccactagt aaaaattgga gtgatattgt caaactaaaa tcagta
//
LOCUS       DAGSHT010000042        22496 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGSHT010000042 DAGSHT010000000
VERSION     DAGSHT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.043"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..537
                     /gene="tuf"
                     /locus_tag="MJB72_001467"
     CDS             <1..537
                     /gene="tuf"
                     /locus_tag="MJB72_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBY8262586.1"
                     /translation="VFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVE
                     MFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGG
                     RHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFS
                     IREGGRTVASGVVATIIE"
     gene            909..1220
                     /gene="rpsJ"
                     /locus_tag="MJB72_001468"
     CDS             909..1220
                     /gene="rpsJ"
                     /locus_tag="MJB72_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBY8262587.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1312..1941
                     /gene="rplC"
                     /locus_tag="MJB72_001469"
     CDS             1312..1941
                     /gene="rplC"
                     /locus_tag="MJB72_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBY8262588.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1971..2594
                     /gene="rplD"
                     /locus_tag="MJB72_001470"
     CDS             1971..2594
                     /gene="rplD"
                     /locus_tag="MJB72_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBY8262589.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2594..2884
                     /gene="rplW"
                     /locus_tag="MJB72_001471"
     CDS             2594..2884
                     /gene="rplW"
                     /locus_tag="MJB72_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBY8262590.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2914..3744
                     /gene="rplB"
                     /locus_tag="MJB72_001472"
     CDS             2914..3744
                     /gene="rplB"
                     /locus_tag="MJB72_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBY8262591.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3779..4060
                     /gene="rpsS"
                     /locus_tag="MJB72_001473"
     CDS             3779..4060
                     /gene="rpsS"
                     /locus_tag="MJB72_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBY8262592.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            4092..4427
                     /gene="rplV"
                     /locus_tag="MJB72_001474"
     CDS             4092..4427
                     /gene="rplV"
                     /locus_tag="MJB72_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBY8262593.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4450..5280
                     /gene="rpsC"
                     /locus_tag="MJB72_001475"
     CDS             4450..5280
                     /gene="rpsC"
                     /locus_tag="MJB72_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBY8262594.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNDNRGNYRGQRPQGGSRPQRTENKGN
                     "
     gene            5316..5747
                     /gene="rplP"
                     /locus_tag="MJB72_001476"
     CDS             5316..5747
                     /gene="rplP"
                     /locus_tag="MJB72_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBY8262595.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5749..5952
                     /gene="rpmC"
                     /locus_tag="MJB72_001477"
     CDS             5749..5952
                     /gene="rpmC"
                     /locus_tag="MJB72_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBY8262596.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5976..6230
                     /gene="rpsQ"
                     /locus_tag="MJB72_001478"
     CDS             5976..6230
                     /gene="rpsQ"
                     /locus_tag="MJB72_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBY8262597.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6256..6624
                     /gene="rplN"
                     /locus_tag="MJB72_001479"
     CDS             6256..6624
                     /gene="rplN"
                     /locus_tag="MJB72_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBY8262598.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6648..6953
                     /gene="rplX"
                     /locus_tag="MJB72_001480"
     CDS             6648..6953
                     /gene="rplX"
                     /locus_tag="MJB72_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBY8262599.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6983..7525
                     /gene="rplE"
                     /locus_tag="MJB72_001481"
     CDS             6983..7525
                     /gene="rplE"
                     /locus_tag="MJB72_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBY8262600.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7543..7728
                     /locus_tag="MJB72_001482"
     CDS             7543..7728
                     /locus_tag="MJB72_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBY8262601.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7761..8159
                     /gene="rpsH"
                     /locus_tag="MJB72_001483"
     CDS             7761..8159
                     /gene="rpsH"
                     /locus_tag="MJB72_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBY8262602.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8190..8732
                     /gene="rplF"
                     /locus_tag="MJB72_001484"
     CDS             8190..8732
                     /gene="rplF"
                     /locus_tag="MJB72_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBY8262603.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8768..9136
                     /gene="rplR"
                     /locus_tag="MJB72_001485"
     CDS             8768..9136
                     /gene="rplR"
                     /locus_tag="MJB72_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBY8262604.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            9157..9666
                     /gene="rpsE"
                     /locus_tag="MJB72_001486"
     CDS             9157..9666
                     /gene="rpsE"
                     /locus_tag="MJB72_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBY8262605.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9681..9866
                     /gene="rpmD"
                     /locus_tag="MJB72_001487"
     CDS             9681..9866
                     /gene="rpmD"
                     /locus_tag="MJB72_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBY8262606.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9899..10342
                     /gene="rplO"
                     /locus_tag="MJB72_001488"
     CDS             9899..10342
                     /gene="rplO"
                     /locus_tag="MJB72_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBY8262607.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10386..11654
                     /gene="secY"
                     /locus_tag="MJB72_001489"
     CDS             10386..11654
                     /gene="secY"
                     /locus_tag="MJB72_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBY8262608.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11671..12321
                     /locus_tag="MJB72_001490"
     CDS             11671..12321
                     /locus_tag="MJB72_001490"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBY8262609.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12322..13068
                     /gene="map"
                     /locus_tag="MJB72_001491"
     CDS             12322..13068
                     /gene="map"
                     /locus_tag="MJB72_001491"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBY8262610.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            13090..13359
                     /locus_tag="MJB72_001492"
     CDS             13090..13359
                     /locus_tag="MJB72_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBY8262611.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13397..13615
                     /gene="infA"
                     /locus_tag="MJB72_001493"
     CDS             13397..13615
                     /gene="infA"
                     /locus_tag="MJB72_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBY8262612.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13651..13764
                     /gene="rpmJ"
                     /locus_tag="MJB72_001494"
     CDS             13651..13764
                     /gene="rpmJ"
                     /locus_tag="MJB72_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBY8262613.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13917..14288
                     /gene="rpsM"
                     /locus_tag="MJB72_001495"
     CDS             13917..14288
                     /gene="rpsM"
                     /locus_tag="MJB72_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBY8262614.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14324..14722
                     /gene="rpsK"
                     /locus_tag="MJB72_001496"
     CDS             14324..14722
                     /gene="rpsK"
                     /locus_tag="MJB72_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBY8262615.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14754..15377
                     /gene="rpsD"
                     /locus_tag="MJB72_001497"
     CDS             14754..15377
                     /gene="rpsD"
                     /locus_tag="MJB72_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBY8262616.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15455..16402
                     /locus_tag="MJB72_001498"
     CDS             15455..16402
                     /locus_tag="MJB72_001498"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBY8262617.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16423..16764
                     /gene="rplQ"
                     /locus_tag="MJB72_001499"
     CDS             16423..16764
                     /gene="rplQ"
                     /locus_tag="MJB72_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBY8262618.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16884..17717
                     /locus_tag="MJB72_001500"
     CDS             16884..17717
                     /locus_tag="MJB72_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBY8262619.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17705..18571
                     /locus_tag="MJB72_001501"
     CDS             17705..18571
                     /locus_tag="MJB72_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBY8262620.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18565..19368
                     /locus_tag="MJB72_001502"
     CDS             18565..19368
                     /locus_tag="MJB72_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBY8262621.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19400..20131
                     /gene="truA"
                     /locus_tag="MJB72_001503"
     CDS             19400..20131
                     /gene="truA"
                     /locus_tag="MJB72_001503"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBY8262622.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20251..20682
                     /gene="rplM"
                     /locus_tag="MJB72_001504"
     CDS             20251..20682
                     /gene="rplM"
                     /locus_tag="MJB72_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBY8262623.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20711..21103
                     /gene="rpsI"
                     /locus_tag="MJB72_001505"
     CDS             20711..21103
                     /gene="rpsI"
                     /locus_tag="MJB72_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBY8262624.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21562..22314
                     /gene="cwlD"
                     /locus_tag="MJB72_001506"
     CDS             21562..22314
                     /gene="cwlD"
                     /locus_tag="MJB72_001506"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBY8262625.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         9054 a         2632 c         4630 g         6180 t
ORIGIN      
        1 gtattctcaa tcacaggaag aggaacagtt gcaacaggaa gagtggaaag aggagtacta
       61 aaagtacaag acgaagtaga attagtagga ttaacagaag caccaagaaa agtagtagta
      121 acaggagtag agatgttcag aaaattatta gaccaagcac aagcagggga taatatagga
      181 gcattattaa gaggagtaca aagaaacgag atagaaagag gacaagtact agcaaagact
      241 ggatcagtaa aggcacacac aaagtttaca gcagaagtat atgtacttaa aaaagaagaa
      301 ggtggaagac atacaccatt ctttgatgga tatagaccac aattctattt cagaacaaca
      361 gacgtaacag gagcttgtaa gttaccagaa ggaatagaga tggtaatgcc tggagataac
      421 gtaacaatgg aagtagactt aataaactca atagttgtag aagagggatt aagattctca
      481 ataagagaag gtggaagaac agtagcttca ggagttgttg ctacaataat agagtaatcc
      541 tttactaaac taatggattt aatataaaaa aaagaggggg taactcctct cttttttgtt
      601 ttttgttgac aagtagcgct aaagcatata tactaaacta gtgccttgag acagattaac
      661 aatcaagttt aaggttatga tgttgtaata gaaaataaaa ttgaaaaaaa cagaaaaaaa
      721 acttgaaaaa atgtaacaga tgatataaaa tataatagtg tgcttgagag attaagaaaa
      781 taataattaa atcctagtga aatacgaact gatatcgatt taaaagttac aacacacccg
      841 gagcagtgta tcggtattgg ttgtacgcaa cttgaaaatt aaacacgtga taaaggaggg
      901 aaaacaaaat ggctaagaat gaaaaaataa gaataagatt aaaatcatat gatcacaaat
      961 tattagattt ttcagcaggt aaaatagttg aaactgctaa aaaggctgga tcacaagttt
     1021 caggacctgt gcctctacca acagaaaaac aagttgtaac tatattaaga gctgtacata
     1081 agtacaaaga ctctagagaa caatttgaaa taagaactca taaaagacta atcgacatag
     1141 ctaatccaac acctaagaca gttgactcat taatgagatt agacttacca gcaggtgtag
     1201 atatagaaat aaaattataa gaacccctaa gatattatcc tatagatgat tatcttagaa
     1261 tgattactct aaaagatgta atccgctgta agaattatag gaggtgctaa tatgaaagga
     1321 atattaggca aaaaagttgg tatgactcaa atattcactg ataaaggtgt agttatacct
     1381 gtaacagctg ttgaagcagg gcctatggtc gttactcaaa taaaaacagt tgataaagat
     1441 ggatacaatg ctatacaaat aggttttgaa gatgctaaag aaaaagcttt aaacaaacct
     1501 aaaaaaggac atttagctgc tgctaatgtt ttaaagaaac atttaaaaga atttagagta
     1561 gattctgtag aaggatacac agtaggacaa gaaataaaag ctgatgtatt tgaagcaggt
     1621 gcaaaaatag atgttactgg aataagtaaa ggtaagggat tccaaggtcc aataaaaaga
     1681 catggacaaa gtagaggtcc tgaaactcac ggttctagat accacagaag accaggttca
     1741 atgggagctt gttcttaccc aggtagagta tttaaaaaca aaaaattagc tggacatatg
     1801 ggaagcgtaa aggtaacagt tcaaaactta gaggttgtta aagtagatgc tgacaagaac
     1861 cttatattag ttaaaggagc tataccagga gctaaaggtt cagtagtaac tataaaagaa
     1921 gctataaagg tttctaaata atgactaacc tagagaaagg aggaataaca atgccaaaat
     1981 taaatgtact aaatgttagt ggacaaaacg ttggagaaat agaattatca gattctatat
     2041 ttggcgtaga agttaatgga catgttttat acgaagttgt aaaaaatcaa ttagcaaata
     2101 agagacaagg aactcaatct gctaaaacta gagcagaagt tagaggtggc ggaagaaaac
     2161 cttggaaaca aaaaggaaca ggtagagcaa gacaaggttc tacaagatct gtacaatggg
     2221 taggtggagg agttgctttt gcacctaaac caagaagcta caaatataca ttacctaaga
     2281 aggtaagaag attagctatg aagagtgctt tatcttcaaa agttcaaaat agtgaagtta
     2341 tagtattaga tgcattaaac atggatgctc ctaagactaa agaatttgct caaatattaa
     2401 acaatataaa tgctgctaaa aaagctttag tagttatagc tgacaaaaac gataacgtaa
     2461 taaaatcagc tagaaatata gaaggtgttc aaactgcatt agttaatact atgaatgtat
     2521 atgatatatt aaaatacgat tcatttataa taactacaga tgctgttaaa aaagtggagg
     2581 aggtgtacgc ataatgacta atccacatga tgtaataata agaccagttg taactgagca
     2641 cagtatggct gaaatgggtg aaaaaaaata cacttttgtt gtggctaaag atgcgaacaa
     2701 aactgaaata aaaaaagcag tagaaaaagt attcggagtt agtgttgata aagtaaatac
     2761 tttaaactac gatggaaaag ttaaaagaat gggtagaact caaggaagaa catcaagctt
     2821 taagaaagca gtagttaaat taactgctga tagtaaagaa atagaattct tccaaggaat
     2881 gtagtattat aacaaacgaa ggagggaaaa cagatggcta taaaaaagtt tagaccaact
     2941 tctcctgcct taagacaaat gacagtttta gtttctgatg aaataacatg taatcaacca
     3001 gaaaaatctc ttttagttaa cttaaagaaa aatgctggta gaaacgtaca tggtagaata
     3061 actgttcgtc atagaggtgg aggacaaaaa agaaaatata gaataataga ttttaaaaga
     3121 gataaagatg gtatacctgc taaggttgct actatagaat atgatccaaa cagaactgca
     3181 aatatcgcat tattaaacta tgctgatgga gaaaaaagat atatattagc tccagttgga
     3241 attaacgttg gagatactat attatcagga ttaggtgctg atataaaacc aggaaactgt
     3301 ttagcattaa aagatatgcc agttggtact atcattcata atatagaatt aaagccagga
     3361 aaaggagctc aattagttag atctgctgga gtttctgcac aattaatggc taaagaagga
     3421 aaaaatgctt tattaagatt accatcaggt gagatgagat tagtaagcat aaactgtaaa
     3481 gctactatag gtcaagttgg aaatatagag catggtaacg tagttatcgg taaagcaggt
     3541 agaaaaagac atatgggaat aagacctact gtaagaggtt ctgtaatgaa ccctaatgac
     3601 catccacacg gtggagggga aggtagatct ccaataggta gaccatctcc tgttactcca
     3661 tggggtaaac cagctcttgg atacaaaact agaaagaaaa ataaagcttc aaataaacta
     3721 atagtatcaa gaagaactaa gtagtaaatt tatttttgct cggaaaggag gaatataaat
     3781 gtcaagatca actaaaaaag ggccttttgt ccatgcaaga cttttaaaga agatagaagc
     3841 tatgaatgct agtggaaata aagaagttat aaaaacttgg tcaagatctt caactgtatt
     3901 tccacaaatg gtagaaaaca ctatagctgt tcatgatgga agaaaacatg ttccagtata
     3961 tataacagaa gatatggttg gacataaatt aggtgagttc gttcctacta gaactttcaa
     4021 aggacataag gatgacgaaa aatctaataa gagaaaataa ttaaattatg actaagaaag
     4081 gaggaataac aatggaagca aaagctactg ctaaatacgt acgtgtatca ccaagaaaag
     4141 caggccaaat atgtgacctt gttagaggaa agaatgttga tgaagcatta gcaatattaa
     4201 agtttactcc aagaggagca gcttcaataa tagctaaggt tgtaaaatca gctaaagcta
     4261 acgcagaaaa caatcacgaa atggatactg aaaaattata tatagcatca atagttgcta
     4321 atcaaggacc aacaatgaag agattcatgc ctagagctat gggtcgtgca actacaataa
     4381 gaaaaagaac ttctcatata gaggttgttg ttaaagaaaa aaaataatta atagatagga
     4441 gggaaagtaa tgggtcaaaa ggttaatcca cacggcttaa gggtcggtgt tataaaagat
     4501 tgggactcaa gatggttcgc tactgataaa aaagagttcg gaaacttatt attagaagac
     4561 cataatatac gtaaattctt aaagaaaaga ttatactcag ctggagttgc taagatagaa
     4621 atagaaagat cagctaacaa aataaaaatg gacttacacg ttgctaagcc tggtgtagtt
     4681 ataggaagag ctggtgcagg aatcgaagca ttaaaagctg aattagaaaa aatgacaaag
     4741 aaaactataa tagttaatat agtagaagta agaagtacag ataaaaatgc tcaattagta
     4801 gctgagaata tagctttagc tatagagaga agggttgctt tcagaagagc aatgaaacaa
     4861 gctatacaaa gagcaatgaa atctggtgct aaagggataa aagtttctgc ttcaggaaga
     4921 ttaggtggag ctgaaatggc tagaactgaa ggttatagtg aaggaaatgt gccactacaa
     4981 actttaagag cagatataga ttatggtttc gctgaagctg atacaactta tggaaaaatc
     5041 ggtataaaag tttggatatg taatggtgaa gttttaccaa ctagagacgg tgtaaatcca
     5101 agagaagaaa gcagaaagag cgatagaaga gacaacaaga gagataatag aagaaacgat
     5161 agaagaggaa atgacagaag aggaaatgat aacagaggaa atgataacag aggaaactac
     5221 agaggacaaa gacctcaagg tggatcaaga cctcaaagaa ctgaaaacaa aggaaattaa
     5281 taatagttag gttcaaggaa ggaggaaaaa tactcatgtt aatgccaaaa agagtaaagc
     5341 gtcgtagagt tcatagagga agcatggctg ggcaagctca taagggtaat aaagttactt
     5401 atggtgagtt tggattagta gcattagaag cttcttggat aacttctaat caaatagaag
     5461 ctgccagaat cgcgatgact agatatataa aaagaggggg aaaggtttgg ataaaaatat
     5521 tccctcataa accagtaaca agaaaaccag cagagactcg tatgggtgca gggaaaggtt
     5581 ctccagaata ttgggtagct gtagttaagc caggaagagt tatgtttgaa ttagcaggtg
     5641 tttctgaaga taaagcaaga gaagctatga gacttgctgc acataaatta ccaatcaaat
     5701 gtaaatttgt taaaaaagaa gatttagaag taaagggtgg tgaataggat gaaagctaaa
     5761 gaattaagag atttaacaag cgaagagcta atgaacaaat taaacgactt caaaagtgaa
     5821 ttatttagct taagattcca attagctact ggtcaattag aaaatacagc tagaataaag
     5881 tttgttaaga aggatatagc aaaagttaaa actgttcttg ctgaaagaaa gttatacgaa
     5941 actagagctt aatttggaaa ggaggctttt taaacatgga aagaggaaga agaaaagtta
     6001 gaataggccg tgttgttagt gataagatgg ataaaacaat agtagtagct gttgaagaat
     6061 tcgtacgtca tccactatat aataaacgtg ttaagagaac taagaaattc aaggctcatg
     6121 atgaaaagaa tatatgtaac atcggagata gagttaaaat aatggaaact agaccattat
     6181 ctaaagataa gagattcaga ctagttgaag ttgttgagaa agttaagtag tttttgaaga
     6241 aggagggatt acgatatgat acaacaagaa tcacgtctaa gagttgctga taactcagga
     6301 gctaaagaac ttttatgtat acgtgttcta ggcggaagta aaagaagata tggtaacata
     6361 ggcgacgtta tagttgctac tgttaaaagt gcaacacctg gtggagttgt aaaaaaaggt
     6421 aaggttgtta aagctgttat agtaagaagc aaacaaggcg taagacgtaa tgacggaagc
     6481 tatatatctt ttgatgaaaa tgctgctgtt ataataaagg acgataaaac tccagtagga
     6541 actcgtatat tcgggcctgt tgctagagag ttaagagaca atgaattcat gaaaatagtt
     6601 tctcttgctc cagaagtact ataattaagg aggtgcaata ggacatgatg cgtgttaaaa
     6661 aaggtgacac tgttgtagtt atagcaggta aagataaagg taaaaaaggt tcagttttaa
     6721 aggtatatcc taaaactagc aaagtgttag ttgaaggtgt aaatgtaata actaaacatc
     6781 aaaaaccaag tgctatgaac caacaaggtg gaataataaa taaagaagcc ccaatacaca
     6841 tatctaatgt aatgccattt gatcctgaaa caggaaaagg cgttagagta agatatgaag
     6901 taaaagatgg gaacaaagta agagtatcag caaagagcgg aaaagaatta taataaatat
     6961 tgcttgaaag gagggacctt aaatggcttc tagattacaa gaaaaataca tgaaagaagt
     7021 tgctcctgct ttaatggaga aatttggata caaaaacgta atggagatac ctaagttaaa
     7081 taaaatagtt attaacatgg gtataggtga cgctagagaa aatccaaaag gattagaaaa
     7141 aggcgttgaa gaattagaaa tgatatcagg acaaaagcct gttataacta aagctagaaa
     7201 atctgttgct aacttcaaat taagagaagg aatgccaata ggaacaaaag ttacattaag
     7261 agctgacaaa atgttctact ttatggacaa attagtttca gtttctttac caagagttag
     7321 agactttaga ggagttaacc ctaatgcttt tgatggtaga ggaaattatg ctttaggagt
     7381 taaagaacaa ttaatattcc ctgaaataga atatgataaa atagataaag taagaggaat
     7441 ggatataata tttgttacta cagcaaaaac tgacgaagaa gctcgtgaat tattaaaatt
     7501 attaggaatg ccattttcta agtaagcaaa ggagggattc ccgtggctag aaaagcgatg
     7561 gttgttaagc aacaaagaaa gcaaaagtac gctactagag aatacactag atgtactata
     7621 tgcggaagac cgcattcagt tttgaaaaaa tttggtatat gccgtatatg ctttagagaa
     7681 ttagcttata aaggtcaaat acctggtgtt agaaaagcaa gctggtagaa gcttgattag
     7741 aatggaagga gggcaacaaa atgacaatga cagatccaat tgcagatatg cttactcgta
     7801 taagaaatgc taatgttgtt aagcatgaaa ctgtagatgt tccagcttct aatatgaaga
     7861 aagaattagc tagaatctta ttagaagaag gtttcataag aggttatgat gttatagaag
     7921 atggaaaaca aggtatcata agaatacaat taaagtacgg acaagaaggc gaaagagtta
     7981 taacaggtct taagaaaata tctaagcctg gaatgagagt ttacgctgct aaccatgaga
     8041 taccaaaagt attaaacgga ttaggaatat cagttatatc tacttcaaaa ggaatattaa
     8101 ctgacaagca agctagaaaa gaaaatgttg gtggagaagt aatctgctac gtttggtaat
     8161 taatatgaac aatgtaagga ggtgcgacca tgtcaagaat aggtgttaag ccaataataa
     8221 taccagcagg tgttgaagtt actatagctg aaggaaattt agttacagta aaaggtccaa
     8281 aaggaacttt aactaaacaa ttaagtgctg agttaaacat aaaaaaagaa gaaaatacaa
     8341 taatggttga aagaccaact gataataaaa aacatagatc tttacacgga ctaactagaa
     8401 ctttattaga taatatggtt gtaggtgtaa atactggttt cgaaaagaaa ttagaattaa
     8461 aaggtgttgg atatagagct caaaaacaag gaaagaaatt agttatgaac ttaggtttct
     8521 ctcatccagt tgagatggaa gatccagaag gaataactgt tgaagctcca aaccaaacag
     8581 agttaatagt aaaaggtata gacaagcaat tagtagggaa ttatgctgct aagataagag
     8641 cttggagaaa gccagagcca tacaaaggta aaggtataaa atacgttgat gaagtaatca
     8701 gacgtaaaga aggaaaaact ggtaaaaaat aataccaaaa cttaaacgct agaaaggagt
     8761 gagctagatg ttaaaaaagg ctgataaaaa tgctaataga cttcaaagac ataagagagt
     8821 tcgtagaaaa ataagcggta cttctcaaag accaagatta tgtgtattta gaagtgctaa
     8881 caatatatat gctcaaataa tagatgacac aaaaagagta actctagttg ctgcatcttc
     8941 tttagaagca gaagttaaga gtgctgtcaa tcatacagga aacaaggaag cagctaagaa
     9001 agttggagaa ttagttgcta aaaaagctgt tgaaaaagga atcactgaag ttgtatttga
     9061 cagaggtgga tacttatatc atggaagaat tcaagaatta gctgaaggtg ctagagaagc
     9121 tggtcttaag ttctaatacg aaggagggaa aaatacatgc tacgtcgtaa gccgatagat
     9181 gcaggacaac ttgacttaca agaaaaagtt gttgaagtaa gacgtgttac taaggttgta
     9241 aaaggtggta gaaatttcag atttgcagca ttagtagttg ttggagatga aaacggacac
     9301 gttggtatag gtgctgggaa agcaatggaa gtaccagacg ctataaaaaa agcagttgaa
     9361 gatgctaaga aaaatctaat agtagtgcca atagttggga ctacaattcc tcacgaagta
     9421 cgcggacact ttggtgctgg aaatatatta ataatgcctg ctgtagaagg tacaggagta
     9481 atagctgggg gacctgctag agccgtactt gagctagctg gactaaaaga cgttagagct
     9541 aaatctttag ggtctaacaa tccaagaaat atggtaaatg ctacaataga aggacttaat
     9601 tctctaaaaa cagttgaaga tatagctaaa cttagaggta aaaaagttga agaacttcta
     9661 gggtaaggag gtagtgtaaa atggctaaat tacaaataaa gttagttaga agtgttatag
     9721 gaactactcc taaccaaaaa aagaatgttg aagcgttagg attaagaaaa agagaacaag
     9781 ttgttgtaaa agaagacaat gctcaaacaa gaggtatgat aaataaagta agtcacttat
     9841 tagaagtaac tgaaatagct gaataatttt tataaagtag tgcaaggagg tgcaatccat
     9901 gaagttacat gagttaaaac ctgctgaagg tgcagtaaga gctaagagaa gattaggtag
     9961 aggtactgca actggacaag gtaaaactgc aggacgtgga caaaaaggtc aatggtctcg
    10021 ttctggtggt ggagtaagag taggatttga aggtggacaa atgccactag ctagaagact
    10081 tcctaagaga ggtttcaata acatatttaa gaaagtttac acagaagtta atgttgaagt
    10141 tttaaacaga tttgaaaatg gaacagaaat aactgcagaa ttattaaaat ctactaaaac
    10201 tataagcaaa ataggtaaag acggaattaa aatcttaggt gaaggtaatt tagagaaagc
    10261 tttaactgtt aaagctgcta aatttacagc ttcagctcaa gaaaaaatag aaaaagctgg
    10321 tggaaaagca gagttagtat aataacctgc taatctccta gccagtacag gcggggtgaa
    10381 ttagcgtgct gtcaaaatta aaacaagctt ggaagattaa agcagtaaga aaaaaagtaa
    10441 tgtatacact tatgatgatc gtgatattca gaataggtac tactatacca gttccaggca
    10501 tagacactag tattatacaa aagatggtag gcggcaatag tctactttct ctgtataata
    10561 tgtttactgg tggagcattt agtaactttt cactatttgc tttaggtata agtccatata
    10621 tcactgcatc aatcataata caacttctaa cagtaggttt tgaaagttta gcagaacttc
    10681 aaaaatctgg tgaagaagga aagaaaaaaa tcaataagta cactaaatat acagcactag
    10741 ctctagcagt tgtacaagct ttgggaatta cattaggaat tgtaagaagt gctttaatat
    10801 caaatagtgt gttttttatc acaactgttg taataacatt agtttcagca agtatgttag
    10861 taatgtggat tggggacaaa attactgaaa aaggaatcgg taatggtagt tcagtaataa
    10921 tatttgcagg tataatatca agaataccta cagatgttat taaaatatcg caacaagtta
    10981 aatctggcga agttgcacct tgggttatag tgattctagc tgtagttata ttacttacag
    11041 taacaggagt tacatttata caagaagcaa ctagaaagat acctgtacaa tatgctaaaa
    11101 gagttgttgg aagaaaaatg tatggaggac aaagttctca tataccaatg aaagttaacc
    11161 agtcaggagt tatgcctata atttttgcta gttcactttt agctttccct caaacaatag
    11221 ctatgtttat gggtccaaat gctcaggctt ttgttcaaaa atacttaagt atggcaacag
    11281 aacaaggatt ttggacatat agatcaattg agattctact tataattttc ttctcatatt
    11341 tttatacaac tgtatcattt aatacagaag acatatctaa aaatatgaaa aataatggtg
    11401 gatttatacc aggtataaga ccaggtgagc ctactatgga ttatttaaat agaattttaa
    11461 ctagattaac tttagctgga gctactttct tagcaataat tgctatggtg ccagctctta
    11521 caacacatta tatgaaagta aatatgagtc tagctgggac ttcattgcta atcgttgttg
    11581 gagttgcttt ggagcttaag agacagttag agtcaaactt agttatgaga agctatcaag
    11641 gtttcttaaa ataaaatgga gatggataat atgagaataa tattacttgg acctccaggt
    11701 gctggtaaag gtactcaggc ggcaggaata gtagaaaaat acaatatacc tcatatatca
    11761 acaggagata tattcagaaa gaatataaaa gagggaacag aacttggaaa aaaagctaaa
    11821 gaatacatgg accaaggttt attagtacca gatgagttaa ctgtaggttt agttactgat
    11881 agaatatctc aagaagattg taaaaatgga tttatgttag atggatttcc aagaaatgta
    11941 gcacaaggag aacatttaga tatcttctta aaaaatgctg gtatatcact agataaagtt
    12001 gtcaatattg aagttgataa gagtatatta gtgtctagag cagttggtag aagaatatgt
    12061 aagtcttgtg gagctactta ccatgttgag tttaatcctc ctaaagtaga aggtgtatgt
    12121 gatgtatgcc aaggagaatt atatcaaaga gctgatgata atgaagaaac tgtatctaag
    12181 agaatacaag tttatctaga tgaaactaag cctttagtag attattatag caaacaaggt
    12241 ataatagcag atataaaagg tgatcaagca atagataaag tatttgaaga tattgtcgca
    12301 gccttaggaa gtggaaaata aatgattatc ttgaaatcaa aaaaagaaat agaactttta
    12361 agagaagcag gtaaaatagt tgctgacact catgaagttt taagaaaagc tatttctccg
    12421 ggaatatcta ctttagaatt agataaaata gctgaagaga atataagaaa atacaatgca
    12481 gaaccttctt ttaaaggata tggaggtttt ccaggaagta tatgtgcttc tatcaataga
    12541 gaggtagtac atggtatccc aggagaaact atattacaag aaggtgacat tgtaagtttg
    12601 gatataggag cctattataa aggatatcat ggtgattctg cgaaaactca cggagtaggc
    12661 atgatatctg aagaagatag gaaattgata gaagtaacta gagaaagctt ctatgaagga
    12721 ataaaatttg ctaaattagg atatagactt tcagatatct cacacgcagt acaaacacac
    12781 gttgagaaac atggtttctc ggtagttaga gatttagtgg gtcatggagt tggagcaaat
    12841 cttcatgaag acccacaagt acctaattat ggactcccag gaaaaggacc aagacttaga
    12901 gaaggaatgg ttattgctat agagccaatg gttaatgcag gtcgttatca tgtaaagact
    12961 ctatcagatg ggtggacgac tgttacaata gatggaaaaa aatcagctca ttatgagcat
    13021 acgatagcaa ttactgaaca tgaacctttg atattgacaa agttgtaatt taaaaagtag
    13081 gtgaaaaaag tgctatcaga taatttaagt ataggtcaag ttgttaaagt ttcattagga
    13141 cgagataaag gaaatttatt ctttgtagtc aaaataatta ataatgaata tgtattaata
    13201 gctgatggaa aaaagagaaa acttgacaaa cctaagttaa agaaagtgaa gcacttaaaa
    13261 aaatataatt ttattaatga tgaagttaga aaaagagtag tatctggaca agagattaca
    13321 gattcttttt taagagctga acttactaag ttaaattaga taatttaact tattgaaatg
    13381 gagggctggt tagttaatgg ccaaaaaaga tgttatagaa ttagaaggta cagtttcaga
    13441 agctttacct aatgctatgt tcaaagttaa actagaaaat ggacatgaga tattatgcca
    13501 catttctgga aagctaagaa tgaacttcat aagaattctt gaaggtgaca aggtgaatgt
    13561 tgaactttct ccatatgacc ttacaagagg aagaattact tggcgtaaga agtagactat
    13621 attaatattt agtctaagga gggattaata atgaaggtta gaccatcagt aaaaccaata
    13681 tgtgaaaaat gtaaagtaat aaaaagaaaa ggaaaagtaa tggttatctg tgaaaatcca
    13741 aagcataagc aaaagcaagg ataagaaatt attactagca ttttgttaaa ttttatagta
    13801 taatactata ttgtgatgtt aacaaaagaa cgttaaaatc gttgaacaat ctggcgtagg
    13861 attggcatat gatgtgaagt tttaaacact aaaaactatg tgtaggaggt gcgatcatgg
    13921 caagaatagc tggggtagat ttacctagag aaaaaagagc ggagataggc ttaacttata
    13981 tatatggtat aggtaaagca actgctaacg aaatattagc taaggctgag ataaatccag
    14041 atactagaat aaaagattta tcagaagatc aagttaatga gttaagaaaa gttatagatg
    14101 acgatttctt agttgaaggt gacttaagaa gagaaattgc tttaaacata aagagattaa
    14161 gagatataaa atgttataga ggaataagac acgctaaagg tcttccatta agaggacaaa
    14221 gaactaagac taatgctaga actagaaaag gtcctagaaa aacagtatct cgtaaaaaga
    14281 aaaaataaat aaataataac tagatgaagg agggaaaagg aaaatggcta aaccaaaaaa
    14341 gaaagttaca cgtattagaa gaagagaacg taaaaatata gaacgtggtc atgctcatat
    14401 acaatcaact ttcaataata caataataac tttaactgac gttcacggaa atgctatatc
    14461 ttgggcaagt tctggacaat taggattcaa aggatcaaga aaatcaactc catttgcatc
    14521 tcaaatggct gctgaaacag ctgcaaaagc tgcgatggaa cacggactaa aaagtgttga
    14581 ggtattcgta aaggggccag gttcaggaag agaagctgca ataagagctt tacaagcaac
    14641 tggactagaa gtaactatga taaaagacgt tactccaatc ccacataacg gatgcagacc
    14701 accaaagaga agaagagtgt aattaataag agtacaatag gaggtgtaga taaatggcaa
    14761 gatatacagg tgcatcatgt agacaatgcc gtagagaggg aatgaaatta ttccttaaag
    14821 gtgatagatg ttatacagac aaatgtgcta tagtaaaaag aaactatgct ccaggtcaac
    14881 atggccaagg aagaaagaaa gtttctaact atggattaca attaagagaa aaacaaaaag
    14941 ttaaaagaat atatggagtt ttagagactc aatttagaaa tttatatgaa cgtgctgaaa
    15001 atatgcctgg taaagcaggt gagaacttat taagtttatt agagagaaga ttagataatg
    15061 tagtttacag aatgggacta gcatcttcta gaaaagaagc tagacaatta gtgactcatg
    15121 gtcatttcac tttaaatgga aataaagtag acataccttc attaatagtt aaggttggag
    15181 atgttataga agttaaagag aaatcaagat cttctgcaaa attcaaaaat ttagtagaag
    15241 ttaactcaag aatagctcct aagtggttag aagctaatgt agaaggaatg acagcaaaag
    15301 tagttggtgt tccaacaaga gaagatatag atcttgagat agcagagcac ttaatcatag
    15361 aactttactc taaataataa gcacactttt aaaaattatt ttaaaatttt gtttaccctc
    15421 agtggattac tgaattttaa ggagggtttt gtccatgata gaaatagaaa agccaaaagt
    15481 agatatagtt gagcttagcg aagactatag atatggtaag tttgttatag agcctctaga
    15541 aagaggatat ggaataacta taggtaacgc attaagaaga atattattat catcattacc
    15601 aggtgtagca gtgaatgcta taaaaataga tggagttctt catgaatttt caacaatacc
    15661 tggtgttaaa gaagatgtta ctgagataat attaacttta aaagagcttt cagcaactat
    15721 agatggtgaa ggaagtagaa cacttaaaat agaagctcaa ggaccatgct ctatcacagg
    15781 tgcagatata atctgtcctc cagatgttga aatattaagt aaagatttag caatagctac
    15841 attagatgat aatgctaaac ttaatatgga gatatttgta gataaaggta gaggttatgt
    15901 ttctgctgaa gaaaataaaa cagagaatgt tccaataggt gttttacctg tggattcaat
    15961 atatactcct gttgagaagg ttagttatca tgtggaaaac acaagagttg gtcaaaaaac
    16021 agattatgat aaattagtat tagaagtttg gactaatggt agtataaatc ctcaagaggg
    16081 aatatcatta gctgcaaagg tattagttga gcatttaaac ttatttatag acttaactga
    16141 acatgtaagt agtgttgaaa tcatggtaga aaaagaagaa gatcaaaaag aaaaagttct
    16201 tgaaatgact atagaagaat tagatttatc agttagatca tataactgtt taaagagagc
    16261 gggaattaat acagttgaag aattagctaa taaatctgag gatgatatga tgaaggttag
    16321 aaacctaggt aagaagtcat tagaagaggt aatacaaaaa ctagaagaac ttggattggg
    16381 attaaaacca agtgaagaat agcgagcaaa ggagggataa gaatggctaa gtaccgtaaa
    16441 ttaggacgtg aaacagctca cagaaacctt atgttaagaa acttagtaac ttgcttacta
    16501 agaagtggaa gaatagaaac tacagtgact agagcgaaag aaactcgtag aatggctgaa
    16561 aagatgataa ctcttgctaa gagaggagat cttcatgcta gaagacaagt tttagcttat
    16621 gttatggatg aaacagtagt taataactta ttcacagatt tagctccaaa atatgctgag
    16681 agaaatggtg gatacactag aataataaaa atagggccaa gaaaaggcga tgctgctgaa
    16741 atggctttta tagaattagt atagtaagga aaggattagg cttgatggct taatcttttt
    16801 tttatttttt tgactaaggt atgatataat aatgtgtaaa atgattttta taagttattg
    16861 cttactaaaa ggagtatgag ttaatggata atatagtaaa ggtaaataat atttcgtttg
    16921 agtatattac agatgaagca aaacttaaag caatagataa tttaagccta gatgttaaaa
    16981 aaggagaatt tgttgcgata ataggacata atggttctgg taagtctact ttatctaaaa
    17041 acttaaatgc tattcttatg cctactgaag gaaatattct tattgatgat atggatacta
    17101 aagaagaaga aagattatgg gatataagac aaactgcagg catggtattt caaaatccag
    17161 ataatcaaat agttgctact atagtagaag aagacgttgc atttggacct gagaacttag
    17221 gtatagaacc aaaagaaata agacgaattg tagaggagtc tttaaaaagt gtaggtatgt
    17281 atgatttaag agatagacag cctcatttgt tatcaggagg tcaaaagcaa agagttgcaa
    17341 tagcaggaat tattgctatg aggcctaaat gtataatatt tgatgaagca acagcaatgt
    17401 tagatccatc tggaagaaaa gaagttatga aaacaataaa aagacttaat aaggaagaaa
    17461 atataactgt tatacatata acgcacttta tggaagaagc agtagaagct gatagagttg
    17521 tagttatgga aaaaggaaag aaaatcttag agggaactcc tagagaagta ttcagtaaaa
    17581 taaagatgtt aaaggaaatc ggtttagatg taccttgtat gacagagtta tctagtttat
    17641 taatagaaga aggtataaat attagtagtg atatattaac tgtggatgag atggtgatgg
    17701 aattatgtca attatagtaa agaatttaac acatatatat aatgaaggga tgccttttgc
    17761 aagtaaggca cttgatgatg tttcttttga gattaaagat agagattttg ttggtcttat
    17821 aggtcataca ggctcaggaa aatctacatt aatacaacat ttaaatggat tactaaagcc
    17881 ttcctcagga gaaatattta taaatgattt taatataaca gataaaaact tgaatttaac
    17941 tgagattaga aaaagagtag gggttgtatt tcaatatcca gaataccaat tgtttgaaga
    18001 aacgattgat aaagatatag cttttggacc ctctaattta ggcttagagg agtctgagat
    18061 acataacagg gtaaaagcat caatggaagc tgtaggactt gattatgagg gatttaaaga
    18121 caaatctcct tttgaattat ctgggggaca aaagcgtaga gtagcaattg ctggagttat
    18181 agccatgaac ccagaagttc tcatattgga tgaaccaact gctggtcttg accctggtgg
    18241 aagagatgaa atttttaact taataaaaga cttacatgaa aaaaagaata tgactataat
    18301 actatcatca catagtatgg atgatatggc aaagttagca aaaactttaa ttgttatgaa
    18361 tcatggtagc gtagaattta tgggaactcc tagagaagta tttaaatcaa atgctagtaa
    18421 attaaaagat ataggattag atattccaca agttttagag cttgcattga aattgagaga
    18481 aaaaggcttt gacatcagtg aggatatatt gactttagaa gaagcaaaac aggaaatatt
    18541 aaaagttgtg agaggacgag gattatgtta aaagatataa ctatagggca atactaccca
    18601 acaagttctg ctattcataa attagatcca aggataaagc ttgtagcgac aattgtattt
    18661 atggtatcta tatttgtagt aaataaattt tggccttata tagtagtttt attatgttta
    18721 ttagctatga taaaattggc taatatacca gtcaaatata ttgttaaggg tgttaaacct
    18781 cttaaatgga ttattttgtt tacatttctt ataaatattt ttttcttacc aggtgatgaa
    18841 atatggtcat ttggattttt agctattaca aaacagggtc taagacaagc aatttttatg
    18901 gcaataagat taatattttt agtagttggt acatcattgc ttactttaac aacttcacca
    18961 atagaattaa ctgatggaat agagagattg ttgaatccat ttaagaaaat tggtttgcca
    19021 gttcatgaac ttgcaatgat gatgacaatt gctttacgtt ttattcctac attgttagat
    19081 gagacagaca aaataatgaa ggctcagatg tctagaggtg cagattttga aagtaagaat
    19141 cttataaata gagcaaagaa tttagtacca cttttggtac ctttatttgt aagtgcattt
    19201 agaagagctg atgaattggc tatggccatg gaggccagat gttacagagg tggacataat
    19261 aggactaaga tgagagagtc tgttatatca agaagggatt atatggcatg tgtatttcag
    19321 gttgtttatc tgggagcaat tatagctact agatttatag caatttaatt aataagttat
    19381 agatataatg aggtaaagta tgagaaatat aaaaattaaa atccaataca atggtaagaa
    19441 ttattgtgga tggcaaaagc aacctgattc tttagggata caaggtacaa ttgaaagggc
    19501 tatatatgat ataacaaaag aagaaaccag tcttataggg tctggtagaa ctgattcagg
    19561 agttcatgct attggtcaga ttgcaaattt taaaattaat tcaggtataa gtatagaaag
    19621 tataccaatg gcactaaatg ccaagttgcc aaaagacata tcagttatag aagcatgtga
    19681 agtaaatgat gattttcact ccagatacag tgcaaaaggt aaaacatata aatatttagt
    19741 atataatagc aagtttagaa atccaatact tagtgagata tcatatcaag taaaatatga
    19801 acttgatttt gataaaatgt gttcagaagc aaaaagttta ttaggaacac atgattttaa
    19861 aggttttatg agttctggtt catctgtaaa agatacagtt agaacgatat atgatattga
    19921 tataagtaaa aaagatgact tgattacttt tgagattagt ggtaatggtt ttttatataa
    19981 catggtgagg attattgttg gaacacttgt tgatatgggt cgtggcagaa ttaacgaacc
    20041 atttttagat ataatacaat caaaaactag gtcaagatgt ggacatacag ctccagcaca
    20101 aggattattt ctcaaaaaag ttcattatta acttgacaca ccaggctaca tgtattaaaa
    20161 tatatgagaa tgtgttaata agtccacttg ccccggactt gacataaacg gtaaaaaaat
    20221 tttatgaaaa gtagttaagg agggacagtt atgaaaagtt atatagctaa gccagctgat
    20281 gtacaaagaa aatggtactt agttgatgct gaaggaaaaa cattaggtcg tttagcaaca
    20341 gaaattgcga cagtattgag aggtaaacat aaaccaacat ttactcctca cgttgatggt
    20401 ggagattttg ttgttgtagt aaatgcagaa aaaatagttt taagtggtaa gaaattagat
    20461 caaaaatatt acagatacca tactggttat gtaggtggat taaaagaaat atcttataga
    20521 gatatgatgg ataagaaacc agaagaagtt atatcacatg ctgtaagcgg tatgttacct
    20581 aaaaataaat taagaagcag aatgatgact agattaagag tatttgcagg tgctgagcac
    20641 actcatgcag ctcaaaatcc agaagtttta aactttaaat aagattaata ggtgaaagga
    20701 ggagtttata atggctaacg ttcaatatta tggaactgga agaagaaaaa gttctgttgc
    20761 tagagtaaga ctagttgcag gtgaaggaaa tatattagta aatggaagag cattagaaaa
    20821 ttattttaat tatgagacat taataagaga tgttaaacaa ccattagttt taactggtaa
    20881 tgaaaataaa tatgatgtta tagtaaaagt tgaaggtgga ggattcactg gacaagctgg
    20941 agctataaga catggaatat ctagagcttt attaaaagct gatttagatt taagacctgc
    21001 tttaaagaaa gaaggtttct taactagaga tgcaagaatg aaggaaagaa agaaatacgg
    21061 attaaaagca gcaagaagag ctccacaatt ctcaaaaaga taattactta tttttttgga
    21121 aaagcaaaat cctttattta ctgttattca gaaagtattg gtattttaaa aatgttgggt
    21181 taagttaatt attgatatat aataaaataa taagatatct tattaggatg tttttcatta
    21241 agaagaacat cctttttatt ttgactattt aataaaaaac atatgctaat aacatttaaa
    21301 ttttattttt ataatattgt aatttaaaag tttatataag gttatatatt ttaagatatt
    21361 atttaacaat ataaattctg aatatacgtt atcaaaattt ataatataaa ctaagtataa
    21421 tttattgtat agtattgaat gttagataaa catagtgaat taaataggga cagaaatata
    21481 atatcattac ataataatat attaatacca ataaataaag atatatcaat attaaaaaaa
    21541 tttaaatgtt tgtactaata tatggaatat tatagtgttt ttataagtat acatatacag
    21601 ggtgatgttg tgagaaagta cataaaacat ataattttta gttttgcaat gatatgtcta
    21661 gtagtagtat ccatatttga aattaaaaat atttctgaag atgttatcaa gtatatgcca
    21721 gtaacaaaca aaactataat tttagatgct ggtcatggag gcattgatcc aggtgcatta
    21781 aataaggata agagtacatc tgaaaaggat attaacctag caataacact taagcttaga
    21841 gagcttatag aatcaagtgg tggccttgta atattaactc gagaagatga cagtagcctt
    21901 tataaagaag aaaataataa aactacaaga caaaaatata atgagaattt gaaaaataga
    21961 aaagaaataa tatctaattc taatgccaat atgtttgtct ctatacatct taatgcattt
    22021 gaacaatcaa aatactatgg agctcaaact ttttatccta aagacaaaca agacagtaaa
    22081 gaattatcga agtgtattca agaagaactt aaaagagtag tagataagac aaacaacaga
    22141 gaagtcaaac caagggatga tatatatctt ttgaaagaaa ataatattcc atcagtactg
    22201 atagaatgtg gttttttatc aaatgaaaaa gagtgtaaac tcttaactga tgaaacatat
    22261 caagaaaaaa tagcatgggc aatctacata ggaatacaaa aatatttaag ttgaaataac
    22321 tataaaaata ggtctaaata tgtaaatagc ttgtattaaa gcagttaaaa tgcattaata
    22381 taggctattt ttattttgac aaaaaaatat ttaaaataaa agttaaaaag ttgttgactt
    22441 agaataatat agatgatatt atatatgagt gcccaaaagg agcaccaaaa taagac
//
LOCUS       DAGSHT010000043        22197 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGSHT010000043 DAGSHT010000000
VERSION     DAGSHT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.044"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            80..316
                     /locus_tag="MJB72_001507"
     CDS             80..316
                     /locus_tag="MJB72_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBY8262626.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            329..1672
                     /gene="yqfD"
                     /locus_tag="MJB72_001508"
     CDS             329..1672
                     /gene="yqfD"
                     /locus_tag="MJB72_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBY8262627.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            1611..2726
                     /locus_tag="MJB72_001509"
     CDS             1611..2726
                     /locus_tag="MJB72_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBY8262628.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            2783..3244
                     /gene="ybeY"
                     /locus_tag="MJB72_001510"
     CDS             2783..3244
                     /gene="ybeY"
                     /locus_tag="MJB72_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBY8262629.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            3250..3969
                     /locus_tag="MJB72_001511"
     CDS             3250..3969
                     /locus_tag="MJB72_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBY8262630.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            4088..4489
                     /locus_tag="MJB72_001512"
     CDS             4088..4489
                     /locus_tag="MJB72_001512"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBY8262631.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            4553..5446
                     /gene="era"
                     /locus_tag="MJB72_001513"
     CDS             4553..5446
                     /gene="era"
                     /locus_tag="MJB72_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBY8262632.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            5458..6837
                     /gene="mgtE"
                     /locus_tag="MJB72_001514"
     CDS             5458..6837
                     /gene="mgtE"
                     /locus_tag="MJB72_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBY8262633.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            6910..7065
                     /locus_tag="MJB72_001515"
     CDS             6910..7065
                     /locus_tag="MJB72_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBY8262634.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7031..7804
                     /gene="recO"
                     /locus_tag="MJB72_001516"
     CDS             7031..7804
                     /gene="recO"
                     /locus_tag="MJB72_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBY8262635.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            7797..8396
                     /locus_tag="MJB72_001517"
     CDS             7797..8396
                     /locus_tag="MJB72_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBY8262636.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            8556..9434
                     /gene="glyQ"
                     /locus_tag="MJB72_001518"
     CDS             8556..9434
                     /gene="glyQ"
                     /locus_tag="MJB72_001518"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBY8262637.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            9434..11500
                     /locus_tag="MJB72_001519"
     CDS             9434..11500
                     /locus_tag="MJB72_001519"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBY8262638.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(11666..13213)
                     /locus_tag="MJB72_001520"
     CDS             complement(11666..13213)
                     /locus_tag="MJB72_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBY8262639.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            13493..14260
                     /locus_tag="MJB72_001521"
     CDS             13493..14260
                     /locus_tag="MJB72_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBY8262640.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            14543..14740
                     /locus_tag="MJB72_001522"
     CDS             14543..14740
                     /locus_tag="MJB72_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8262641.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            14769..15845
                     /gene="vorB"
                     /locus_tag="MJB72_001523"
     CDS             14769..15845
                     /gene="vorB"
                     /locus_tag="MJB72_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY8262642.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            15848..16567
                     /locus_tag="MJB72_001524"
     CDS             15848..16567
                     /locus_tag="MJB72_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY8262643.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            16580..17119
                     /locus_tag="MJB72_001525"
     CDS             16580..17119
                     /locus_tag="MJB72_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY8262644.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            17276..18334
                     /gene="buk"
                     /locus_tag="MJB72_001526"
     CDS             17276..18334
                     /gene="buk"
                     /locus_tag="MJB72_001526"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY8262645.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            18352..19245
                     /locus_tag="MJB72_001527"
     CDS             18352..19245
                     /locus_tag="MJB72_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBY8262646.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            19275..20483
                     /locus_tag="MJB72_001528"
     CDS             19275..20483
                     /locus_tag="MJB72_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY8262647.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            20507..21766
                     /locus_tag="MJB72_001529"
     CDS             20507..21766
                     /locus_tag="MJB72_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8262648.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         8702 a         2426 c         4130 g         6939 t
ORIGIN      
        1 taaaactaat ctgttacaac atcaataaag tgcataaata cctatttcaa gcataaaatt
       61 aattaagggg gtaatgctta tgttagaaat atccactgat ttacaagtaa atcaaccagt
      121 tattactgtt acaagtaata cgtttattag tatagaaaat tatctatcaa tattagaata
      181 tgaagttgat ttaataagaa taaagactaa agttaaaaca attaaaatat ctggggataa
      241 actttcactt aagtatatta ctgatagtga gataggtata aaagggatta tctacaatgt
      301 tgagtatgtt gattaggagg agatacactt gataagtttt ataaggggat actatgttat
      361 agttgtggaa ggagtaggac tcgaacaatt tttaaatcat cttataagaa atgggataag
      421 tgtttacaat gtaactagaa taaaaaatac taaaatggag tttcatatag atagacaaga
      481 tataaaagac tttaagaatg tttatagagg aagtaagttt gatataaagg taaagcaaaa
      541 aactggggtc ccttttatta tcaaaagagt ttataagcac aaaggtatgt ggatttgtgc
      601 tcttgtttct ttatttttat tgatgtctac atcacagttt gtgacagatg tatatataca
      661 atctcctgaa ggtataaaaa aagaagctct tagaaatgag ctttataagg ttggagtaag
      721 gccaggagtc tataagaaga gtattgatag aaaagaagtt agagaccata tgatgtcaaa
      781 atttaatgat gtggcatatt tatctatcaa tgtaaaagga acaaatatat ttgttactgt
      841 tacaaaaaag gcagaatccc taaaatctac agaccaatca aattattgta atgtaattgc
      901 tttaaaaaat ggtataatag aaaaggtaat ccctagaagt ggtaaatcag ttattaaatc
      961 tggagacata gtccaaaagg gagatgtctt attaaatgga gctaatacca agtcaatacc
     1021 agaagtatgg gcaagtactt tttatgagtc cacaaaaaag gcaagttatg tagatactgt
     1081 aaataaaaaa actggagaaa agaaaaatat atatacacta agtttttatg ataaagagtt
     1141 tacaatgaga aaaaatataa aatataaaga ttatgttgtt gaaaataaag aaaaaaaact
     1201 atcaatagga aactatactt ttccaataaa gataaaaacg agtacttttt atgaaacaaa
     1261 aaaggttgaa gtaaaaagga ataaagaaga gttaaaaaaa gaattatctg aaaaagcttt
     1321 aaaagagtta gaatacataa tcccagcttc ggctagaata atagatgtaa aacataactt
     1381 taaggttaat aaaaatatgt tagaatattt aataacagtt caaactagtg aaaatattgc
     1441 taaaatttat cctcttagta agtcagaagc agaaaggttt ataaaggaag aaagtaaacc
     1501 agatgaaggt gaagaggaag ttccttccaa tccagaaaaa agaccattaa atgatataag
     1561 aaatgagttt gatgaggata ataaggataa taaagaccaa aacaattctg atgaaaataa
     1621 tagtaatcaa aatagtaata atagtcaaaa taataatagc aataacaact aataaaatta
     1681 aacttaaaat cttaggagga ctaattcaat tgataataca aaagaaattt acaatagcag
     1741 atggtaactt tgaaagagag ttatttggca actttgatga aaatgtaaaa ttaatagaga
     1801 agactttaaa tatagatgta atacttagag aaggcaatat aattttaatt ggagaagaaa
     1861 aaaatgtaga ttctgcatta aaacttatga atgaattaca ccaaactgta tcaaatggta
     1921 agcatttaga taagcagagt atttcttatt cattatctct tttgcttgaa ggaagtgaac
     1981 aaaaaataaa agaacttgag gggacaatcg tcataactca aaggggaaaa gctgttcaac
     2041 ctaaaacttt aggtcaaaaa gaatatataa aacttataga aaataatgat ataacttttg
     2101 gtgttggacc tgctggaaca ggaaagactt atcttgcggt tgctatggca gtaaaagctt
     2161 ttaaaagaga tgaagttagt agaattatac ttacaagacc agctgtggaa gcaggagaga
     2221 gtctaggttt tttaccagga gatttaaagg acaaagttga cccatattta agaccactat
     2281 atgatgcctt atttgaaatg ttgggtgctg ataaatttaa taagtattta gaaagaggta
     2341 ctattgaggt tgcaccactt gcatttatga gaggtagaac acttgataat tcatttataa
     2401 tattggatga agctcaaaat actacttctg aacaaatgaa aatgttttta actagacttg
     2461 gatttggctc taaagctgta gttacaggag atgtaactca aacagattta ccacaaaata
     2521 agaagagtgg tctaatacaa gctacagaga ttcttaaagg tgttccaggt attgggtcta
     2581 taatgctaac agatagagat gttgtaagac atgagttggt acagagaata ataagagctt
     2641 atgagaagca cgacaaaaga gaagaattta aaaaagaaga acgtaaaaaa gcgaaaatgc
     2701 aggaaaagac tttcaaaaga aagtaaaagt gtagtaatat agatatatag cattattaat
     2761 gaataaaaag taggtgtagt gaatggattt gatactagat gatagacaag ataaattaga
     2821 agtaagtgag gagttaattg aaaagataaa ggatataatc atagaatgtt tagattatga
     2881 aggatatgat gataattatg aagtgagtct ttcttttgta gataataaag agattcatga
     2941 attaaataga gaatatagag gagttgatag agtgactgat gttttatcat ttcctctttt
     3001 aagtgatgat tttgaagatg ttgagttaga agaagaatcc ctaggtgaca tagtagtatc
     3061 tcttgaaaga gccttagaac agagtataga gtataaccat agttttgaaa gagaagtatg
     3121 ttttttaata tgtcatagta tgtttcatct tttaggttat gaccacgata cagatgaaaa
     3181 tacaaaagaa atgagagaaa aagaagaaca catattaaat aagttaaaca taacaaggga
     3241 gtagttttta tgaaaccaga aaaaactcgt caaggtataa ttaaagcatt taatgcagct
     3301 atagaaggga tattgtatac atttaaattt gaaagaaata tgaaaataca ttatttaggt
     3361 tcagttgctg ttcttataat aagtttgttt tttaactttt caaaattaga aatgataatg
     3421 ttacttatgt ccatatgttt agttgtagtt gctgaaatgt ttaatacagc tatagaaaaa
     3481 gctgttgatt tagtaactga tgaatatcat gttcttgcta agatagcaaa agatgttgca
     3541 gctggaggcg ttctagtggc agcactaaat tcagtagtag ttggatatat tttgttttat
     3601 gataaattga cagatatatc tggaatatta atttataaaa taagagaatc agaattacat
     3661 ataaccttga tatgtatatt attggttctt atagctgttg tagttgtaaa agcattgact
     3721 tctacaggta ctcctctaaa aggaggaatg ccaagtggtc atgctgcctt agcatttgct
     3781 atagcaactg caattacact tatgactgaa agagttgttg cctctactct tgcatatata
     3841 atggctgtat tagttgccca aagtagaatt gaaggaaaaa tacatacatt ttgggagact
     3901 attgctggag ctttgttagg tgtgttgata gctatattag tatttcagct tggaatgttc
     3961 tataattaaa ataaatgtac ataaaatatt taaaataaaa caaatctgtg ctaattaaat
     4021 ttttatgata taatgtagag tattaaataa aaataacttt taattattgt atacaggagg
     4081 agttgttgtg gataacatag aattgttaag gttggcagaa gatgccagac aacattctta
     4141 tgctccttac tcaggtttta gagtaggtgc agctctttta actaagagtg gtaaagttta
     4201 tactggatgt aatatagaat gtgcatcatt aggtggaaca aattgtgcag aaagaacagc
     4261 tatttttaaa gcaatatcag agggtgataa ggatatctat aaaatagcta tagcaagtga
     4321 taactcagaa aataatgagc aaacatatcc atgtggaata tgcaggcagg taattataga
     4381 gtttggttct gatataaaaa taataacggg atatactaaa ggagaggtgt ttgagcatag
     4441 tataaaggat ttgttgccaa actatttctc tggaaatgat ttaaaataaa tttaaaagta
     4501 taaataaatt taagtaatta aaacaagaag ataaaaagtt aggagacagt taatgttcaa
     4561 atcaggtttt gtcagtatag tgggtagacc aaatgtagga aaatctactt taatgaataa
     4621 tgtagttgga gaaaaaatag ctataatgag tgataagcct caaactacaa gaaatacaat
     4681 acaagcagta tatacagatg aagaaatgca gatagtattc ttagatacac caggtattca
     4741 caaaccaaaa aataaattag gtgaatttat ggtaaaggca gcaacagaag cttttaaaaa
     4801 tgtagattta atattatttg tagttgatga ttctaaaaaa ataggtcctg gagatagaaa
     4861 gataatagag gatttaagaa gtgttaagac accaataatt ttagttgtaa acaaaataga
     4921 ccaactagac caaaaagatg agctatttga tataatcaaa atgtatgata gagaaggtat
     4981 ttttaaagaa atagtaccaa tatcagcatt aaaaggaaag aatacggata ccttaataaa
     5041 agttatacaa aattatttag aagaagggcc aaagtatttt ccagattata tgattacaga
     5101 ccagccagaa agagtattaa tagcagaact tataagagaa aaagttcttc actatcttaa
     5161 tgatgaaata cctcatggtg tagctgttga aattgaaaaa atgaaagcga gaaatgataa
     5221 ggaaatagta gatgtatctg ctgtaatata ctgtgaaaga gattcacata agggaataat
     5281 aataggaaaa aatggtagga aactaaaagg aataggtaag tcagcaagac aagatataga
     5341 attgctttta ggttcacaaa taaatcttca attatgggtt aaagtcaagg aaaattggag
     5401 aaatttacaa aattatataa ataattttgg atataatgat aaataaaggt gggttatatg
     5461 gataggaaac tggatgcttc ccaagattta ttatatgaag taaaatcatt aattgataat
     5521 aataaagtat tggagttaag agaattaata gaggaatatc atataattga tatattcgat
     5581 ataatggaaa atttagagga agacatgaaa attcaattat ttgaagtact tcctttagat
     5641 atggcatctt ctatattaga agaaggtagt gtagagtttt ttattagtat tttatctaaa
     5701 ctggatgtag aacactcgaa aaatatactt gaacttatgt ctcttggaga tatggcagac
     5761 aagttgagtg aacttgaaga agaagaaaga gaacacataa taaatctttt gaatcaagag
     5821 aatgccgatt atgtaaaaga gttactattc tatgatgaag attctgctgg tggaacaatg
     5881 accactggat atatttctat aaataaagat atgacagctc ttgaggctat agaccatatg
     5941 agagaagaag ctgaagaagc tgaaactatt tattatatat atgtggttga tgatgaagaa
     6001 aaactagttg gagtattgtc attaagagaa ttaataatag ctagagacgc aaatatagta
     6061 gaagatttga tgagtgaaaa tataatctct gtttatgtag atgaagatag agaagaagca
     6121 gtaagattag tatctaaata caatttaata gcaattccag tagtagacag acaagaaaaa
     6181 ttaaaaggaa taataacagt agatgatata attgatgtaa tggaagaaga agcaactgaa
     6241 gatatgtata aatttgcagg aagttcagaa catgaaagag aagttgcgga aaaagaaaac
     6301 cctacattga gagagcaaat aatatcagca cttagaggac gattaccttg gttaattatt
     6361 accttagttg gagggttgtt ggcttcgtta atactttcta acttagacta tataatgaat
     6421 cctgtatatg ctccattagt attttttata cctgtagtaa ttggtatggg tggaaatatt
     6481 ggaactcaat catcatctgt tacagtaata acattgtcta ataaggattt aaattttagc
     6541 aatgtggtta gagaaggtat tgtaggtatt attacaggtc ttttgtgtag tataataaca
     6601 ggtattgtaa tttactttgt tatgagaaag ttagacattg tattaattgt ttcaatatca
     6661 ttatttataa atatggtttt aggtgcaaca ataggtgcgt ttatgcctgt tttattaaaa
     6721 aaaatggatg cagacccatc tacagtttca tcaccaatta tatctacagc tcttgatata
     6781 actggtatag cagtttactt tataataaca acggcattat tgtcaaaaat tgtttaataa
     6841 ctttatgtat gatttatcct cttcttacta aaaatataag tataaatgta agaagggggt
     6901 tttttaaaaa tgaacaatat cagttgggaa gtttttaaaa aaacaggtag tatagatgca
     6961 tatttatatt tctgtgattg caaaaacata catgaagaaa ttgaagaagt aagggagaaa
     7021 aaagaagacg atgataatcc tgaacaccca ggggatagta cttaaagcta taaggtataa
     7081 ggaaagtgat ataatactta cactatttac aagaaagctt ggaaaagtat ctgcaatagc
     7141 aaaaggagct aaaaaaaata agagctcttt gctttcatca tcacagttat tttcttatag
     7201 caattttacc ttaaaaaaac aaggtaatat gtacaaggtt actcaaagtg aaataataaa
     7261 aagtttttat aatatatcct atgatataga ggcattttct tatgctacat atattacaaa
     7321 attagttgaa aattctatac ttgaaaatca gactaataat aggctattta tattacttgc
     7381 gcagactctg tatttgtata cacaggataa tacagacaat aggtttataa cagcagcatt
     7441 cgagttaaaa tttttagact atataggatt taaacctata gtaaataaat gcattaattg
     7501 tgaatgtaaa attttacaaa attctgtgtt taatatctac gaaggtggta tactatgtag
     7561 taagtgtagt aaactttttg ataataatat aaaattagac ttaacaacta taagtcttat
     7621 ggaatatgta ttaagaaatg atattttaac atgtagtaaa gctaaagtat caaaatatat
     7681 aacacatgaa cttgagaata ttctagacaa gtatttgaaa gtttatgttg ataatattaa
     7741 ttttaaatca ttacacgtat tgcaaagtgt aaaaaataat aagggagtgg ataatgatga
     7801 gtagtaatat aactattgaa atggttgatt cagtctttga aagagttcca ggagcaactt
     7861 atgcagaagc aaaagaagca ttagtaatgt gcaatggaga tgtaattgag gcagtaatat
     7921 atttagaaaa taaaaaaaat acttgtaatt gtaaagcaaa atctgctaaa gaaactatgg
     7981 aagaagtatt tggcaaagat ggagaaaata taaagtttca attaaaagag cttcttagaa
     8041 aaagtagtgt agtgcgaata atagttgaaa aagatggaaa aactatgatg aacattcctt
     8101 taacagttgg agttgttgga ttagcttttg taccattagc tactttagtt ggattatcag
     8161 ctgctgttat aagtaaatat cgtataaaag ttcaaaatga agaagatgga gaaattgtag
     8221 atttaggtga attgaatgaa gaaaaacttc acgttttgaa gaatatgata actaatgcag
     8281 caaaagatgt gaaggatgta gtagttgaca aaaaagaaga tgacaaagat gtaactgctg
     8341 accttatgaa agaagaatct gaaaagaata aagaagacga agacgaaaat aaatagatat
     8401 aaagtgactt ttaaaaaatg aagagaagat aattataaga attatcacta acattgacaa
     8461 attttataga atttgatatt ctaaaagttg tagaagaact attaaaatag cctttcgtgt
     8521 agacgagagg ctttattgtt ataggaggga tttttatgaa ttttcaagag atgatactgg
     8581 cgttgcaaaa atactggtca aagcaaggtt gtatcatgat gcagccttat gatattgaaa
     8641 aaggtgcagg aactatgaat cctaatactt ttttaagatc attaggacct gagccttggc
     8701 aagtatgcta tgtagagcct tcaagaagac cagcagatgg aagatatggt gaaaacccaa
     8761 acagactata tcaacatcat caattccaag ttatactaaa accttctcca gataatatac
     8821 aagaattata tttggaaagt ttaaaagaga ttggaattga cccaagtgag catgacataa
     8881 gatttgttga agataactgg gaagctgcta cagttggtgc ttggggactt ggatgggaag
     8941 tttggctaga tggtatggaa ataactcaat ttacgtattt tcaacaagta ggtaatattg
     9001 aatgtgagct tgagacagga gaaattacat atggattaga aagattagct atgtacattc
     9061 aagaagttga tagtgtttat gatttaaaat ggaatgataa aataacttat ggtgaagtat
     9121 ttaataaagc tgagtatgaa aactcaatgt acgcatttga attatgtgat gcagatatgt
     9181 tgtttaatct gtttgatata tatgaaaaag aagcattaag acttatggaa aatggccttg
     9241 taataccttc atatgactat gtgttaaaat gctctcatgc atttaataca ttggatgcaa
     9301 gaggtgcaat aggagttagt caaagagctt catttatagg tagagttaga aatatggcaa
     9361 agactgttgc agaaactttt gtaaaacaaa gagaagaaat gggattccct cttttaaagg
     9421 acggtgacaa ataatgaaaa attatctttt atttgaaatc ggtgttgaag aaatgccatc
     9481 aagatttgta ggcagtacac tagaacaatt aaaaaacaat cttagtaaat tatttgatga
     9541 aaatagaata gcttttgata aaataaatgc gtatgggaca ccaagaagac ttactttagt
     9601 agtcgaagga ttgagtgata gacaaaatga tttagaagag gaaataaagg gtcctgctaa
     9661 gaaaattgct gttgatgcag aaaataattt aacaaaacca gcattaggat ttataaaaag
     9721 caaaggatta agcgaatctg acatatattt taaacaagtt ggaaaagaag aatatatatt
     9781 tgcgactata aagcaagatg gaaaagaaac ttcagaagta ctaaaagaca tattaccaga
     9841 gactataaaa tctgtagtat tcccaaaagc tatgcgttgg ggaggaaaaa atcttaaatt
     9901 tgcaagacct ataagatgga tagttgcatt gttaaatgat aaagtattag agtttgattt
     9961 agaaggaata atatcttcta atgtaacaaa aggtcataga tttttaggtg aaagtacaat
    10021 agaagttgat tctatagaag attattttga aaaattagaa aaaaattata tagttttaga
    10081 ccaaaacaag agaaaagaaa tgataagaaa acaatgtata gaagtggcaa attctttagg
    10141 tggagaagtt gaatttgatg aagatttact tgaagaggta acttacttag ttgaatatcc
    10201 aactgctttt tatggagaat ttgatgtgga ttatataaaa ctaccaaaag aagttgttat
    10261 aactcctatg aaacaacatc aaagatattt tccagtactt aaagaaggaa aactgttacc
    10321 taactttata gctgttagaa atggtgattc ttacagaata gacaatgtta aagctggaaa
    10381 tgaaaaagtt ttagaagcaa gacttgcaga tgctttattt ttctatagag aagatactaa
    10441 gagaaactta gaaagctaca tagaaaaatt aaaaactgtt gtattccaag taaaactagg
    10501 aactatttac gacaaaactt taagacttga aaaattaagt gctgatatac ttgatagttt
    10561 aggtttagta gatgaaaagg cagatactat aagggctgct aaattatcta aggcagattt
    10621 agttactgga atggtaggag aatttgatga attacaaggt tttatgggaa aagaatacgc
    10681 aaaagttgga ggagaaaatg atgttgtaag tgaagctatt tttgaacatt atcttccaag
    10741 atttgcagga gatatacttc ctaagactaa tgcaggagtt gccttatcaa tagctgataa
    10801 gttggattca atagcaggtt tctttgctat aggaatacaa ccaacaggtt ctcaagaccc
    10861 atatgcttta agaagacaag ctttaggtat acttagtata cttatggaca gaaaagttgc
    10921 tgtggatata aaagtactta ttgaacatgc tttagacaat tattctgaat tagattttaa
    10981 taaagaagaa gttgttgagc aaataatgaa tttcttcaat gagagagtaa aaaatctatt
    11041 taaagattta ggaataagat atgatgttgt tgatgctgtt ttaagttctg atataaatga
    11101 tgtttctgat atgcatatga gagctttaga gcttaataat tggctagaaa aagatgaatt
    11161 agtggagatg cttactgcat ttaatagagt ttctactcta gctcaaaaag cagaaagtga
    11221 taaaattgat gaatctttat taaaagaaga tgctgaaatt aaactttaca atgaatttaa
    11281 acaagtaaag gtaaaagtta atgaattatt aaaggataag aaatatggta ttgctttaga
    11341 tgaatttgca tctttaagac cagtagttga taatttattt gatacagtta tggttatgga
    11401 taatgatgaa gctattaaga acaatagact tgctttactt aaacaagtat atgatactat
    11461 gctaggaatt tgtgatttat ctaaaattgt ttataaataa aatttaaact atttataaga
    11521 aataaggctg tctcaaaata gagatagtct tatttttcat tgttgagatg atgaaaatat
    11581 atttgatttc atagaaaaag agtgtctcat aaactaaaaa gttcattttg agacacccat
    11641 gctttttgta agtaaatata ataaattata ttttatactt atcagttatt ttgtttaaaa
    11701 tattatcatt tgaatttacc cattcataaa aattgcctga tgatgcgtcc gatattttat
    11761 aaagcatttc aggaatatca ctagctttaa aatctccaag tgattttccc aatctagtct
    11821 tacctatttc aaaactgcct ccaacaaata attcaaatgc atctgtagat ataccatcaa
    11881 cattcttctt tttaccacat aaacctatgc taccaacttg atgaacacca catgaattta
    11941 aacatcctga tatatataac ttaggtgctt tatttaaaat ttctttatta ttttgtaatc
    12001 taaaataatc tatgatttca tgtaacattt tctgactatt ttgtatgccc atttgacaaa
    12061 ttggaacacc tatgcaagat atactttgtt ctaattgaga attacaacta atagttttac
    12121 ttatttctaa tattcttttt gcttcatttc catttaaatt taaaatatat aatccctctg
    12181 tcattcctag tctaatcata ggattttcaa tattatctaa ttctttaagt aaagtactta
    12241 aatcctttgt taacaaaata cctccaactg gatgtatata tacagtgtat aaaccatcct
    12301 gtttttgagg tattagtcta gaatcctgta tatctatttt aaccccaggt ttagagtaat
    12361 ctatttcttc aatattgagt tcaagactac cattttcttt ttctaattta tagtattctt
    12421 taaatttttc taagaataat ttttcaccaa gtgattcgac catatatcta accctagctc
    12481 tgtttttatt ttcataattt ccataatcca taaagaattt aatcatacct tcaacacaat
    12541 ataaggcatc tttcggtttt ataagttcat caagttctaa agcaactttg ggttgtttac
    12601 ctagacctcc acctaagaaa actctgaaat atggttcatc atttttcaaa gtagcaacaa
    12661 atcctaagtc ttgtatacta caatgagcag tatctgtgta acaattagaa taagaaactt
    12721 ttaattttct aggtaaatgg tatgtagtag catttttgat aaaatattta tcagtagcaa
    12781 ctgcatatgg agtaacatca aagacttcac tagggtcaac tccagataat ggagataatc
    12841 cgacatttct aggataatta ccaccaccac ctctagtgaa tatatcattt tttattcctt
    12901 cttccatgat atcaacaatt gaatttatag ataaatcgtg taattgtata gcttgtctag
    12961 ttgttaagtg tatcttatct aagttgtgtt taatagctat ttcatagatt ttattaagtt
    13021 gatactgaga taaaactcca gcagatactc taagtcttat cataaaagat tttttgtctc
    13081 tttgagcata aacaccatat ccaccagaaa atcccttata ttgcatttta tttatttctc
    13141 catttaaaaa ttttgaagaa tattctttaa aattaggtag ttcatttaac aatatctttt
    13201 taaaattatc cattatttca cctcattgtt agtcttgtat aaaaatatat ctattgtata
    13261 atagatatat atattattat ctagtcagga taaaaatata acagaataat tctattaaaa
    13321 gtaagaataa taaggacata gtgagtattt tgtttttatt ttacaaatta agtataatat
    13381 ttttgtttta taaaatataa ttaagataaa tagataattt tgtaatttta atatataata
    13441 tttagtagag tgtgggagta gcacttaata tttgcaggag gagtttttgt atatgggaga
    13501 aataataaat gcattagata gagccctaga cataattttg ctgttatatc atgaaaagag
    13561 agaaatggga ataactgaaa taagcaaggc tatgggagta tataaaagta ctgtacatag
    13621 aactttggtt actcttgaaa acaaaggttt tgttatacaa aatgcagaga atggtaagta
    13681 ttggctagga ataaatttat atgcaatagg tatggttgtg ggagaaaaaa tgtcattgac
    13741 tgaaatagta aaaccatata cgaaaaaatt aaatcaagaa tttaacgaag tagttaatgt
    13801 atctatatta gaagaaagag ctcaagattc tccaagaagt atcataatac ataaagagta
    13861 tggttcaaat caattacttt cagtaaatcc atccgttggt tcaagtagtg aatgttattg
    13921 ttcagcagtt ggtaaatgtc ttatggcatt taatgattct atagattttg aaaaatatag
    13981 aaaaacacca atacacaagt atacagaaca tacaattgat aattgggatg atatgatgct
    14041 ttttttagca aaaataaaag aacaaggata tgctatagat gatgaagaac tagaacatgg
    14101 actaacttgt attggagcac ctatcttaga taaaaataat aaagctattg cagccataag
    14161 tttgtcaggt ccaactataa gaatgagaga aggggacttt gagtataaga ttaaaagagt
    14221 tatagaaact gcaaaaagta tatctgaact ttttagatga tttttaatct cgataataaa
    14281 aataggctat atttaaattg ttatttgtaa atatcagttt aaatgtagcc tatttttctt
    14341 taatctaaaa ttaaatattc ttaaaattta aaatgttatt gaaatatata agcgaatgtg
    14401 ttatctttta attaagcaat aaaaatagaa caaggttcta tactatagaa caaaattgat
    14461 gcatatatag tatttataca aaatataaat attatatatt taaaattaag taaaaatatt
    14521 acataaaggg gagatttgag aaatggaaaa agtcaagttg agtgttgaaa gatgtaaggg
    14581 atgctatctt tgtattgaag cttgtaaaaa agatgcaata agtctttctg actacacaaa
    14641 taaaaaaggc tttgtaacta ttaaagtcga tgaagaaaaa tgtattcaat gtggcgcttg
    14701 ttatacaatg tgccctgatt tagtatttga gatattataa attattttta ttagagggga
    14761 gaaaaggtat gcaaagttta ttaattaaag gaaatgaagc tttagcagaa ggtgctatac
    14821 gtggtggctg cagattttat gcaggttatc caataacgcc acaatcagaa atattagaaa
    14881 tgatggcatg gagacaaaaa gaagttggag gagtatttat ccaaggtgaa tcagaaatag
    14941 ccagtgtaaa tatggttatg ggagcaagtg cacttggagc aagagctatg acttcttcat
    15001 ctggtccagg attttcatta aaacaagaag gtattgcata ttgggtatca gctggtttgc
    15061 cagcagttgt agcatgtgtg caaagatatg gtataggtga tggatttata ggagctggtc
    15121 aagataatta ttggcaagca gtaaaaggcg gtggaaatgg agattatcat ttaatcgtgt
    15181 tagcacctaa tagtgttcaa gaaaatatgg atatggcata tgaatctttt gaattggctg
    15241 aaaaatatat gcatccagta cttatacttt ctgatgctac aataggtcaa atggtagaac
    15301 cttgtatagc accaccaatg aaagaatacg atatggataa agctccatgg gctgcaaaag
    15361 gaacacctgt aggagaaaaa aataaagtaa taactgatat aacatatttt gaagaccaag
    15421 atacatggca aaaagggtat atagaaaaaa tgagaaaagt tcaagaagat gaacaaagat
    15481 gggaagagat agaaatagaa gatgctgaag tggtatttgt ggcatacggt atttcatcaa
    15541 gaatagcaga aggtgcagtt agagatgcta gagcaaaagg aatgaaatta ggtctaataa
    15601 gaccaaaaac attgtggcct tacccaagaa aagcatttaa gaaattaagt gattcattga
    15661 agggacttgt aacagttgaa atgaacatga tggcacagat gaaggaagat gtaattatag
    15721 catgtgataa tagatttcca gtatattcag tagcaacagc tcaatatatg gcagaagtaa
    15781 atcaattaat agattttgct caacagatta tagatggaaa agctaagcaa gaggaggtat
    15841 tttaattatg gctaaaaaag caccagcaat aatgatgtgt gaaaatatgt tctgtggagg
    15901 ctgtggacac tctatagtcg atagaattat tggagaagta ttaacagaaa tggatatatg
    15961 tcaagataca attatatgtt cagatatagg ttgtaatcat atgtttcaat ttagtatgaa
    16021 tgtagatgtt gtcgttccac ctcatggtaa aatgggagca gcaatggcag ctatgaaaag
    16081 agttagacca gataaatata tatttacaat agctggagat ggtggagctt atgcaatagg
    16141 gcttggagaa actatgtcag ctgctactag aaatgataat gtaatatttc ttgttatgaa
    16201 caataatatg tttgcaatga caggagggca aatggctcca actactatag aaggtcaata
    16261 tacagcttct actccagcgg gtagaaatta cgcagatcat ggttcaacat ttgatgttgt
    16321 aaaagttatg ggaaatttag atatagcata tctagcaaga ggaacaataa ctaaccctaa
    16381 agaaataaat acaacaaaaa gatacatcaa aaaagctcta gaaaaacaaa aagaaaagaa
    16441 aggtttttgc ttaatagaag tgcttgtacc atgtccaaca aactggggat taaagccagt
    16501 agatgctatg aagagaattg aaaatgaact tgttccaaag tatccacttg gagaatttat
    16561 tgattaggga ggaaataata tgttgaatga aattatatgt gctggttttg gaggacaggg
    16621 agttcttaca gcaggaaaaa ttataatgta tgttgcatat aaggcagaaa agaaaattac
    16681 atggttccca tcttatggaa atgagatgag gggaggaagt gcaaattgca atgttgttgt
    16741 aagtgatgaa aaaattgcaa atccttgtgt aaaaaatcca gatatactat tgggaatgaa
    16801 cagcgtatca gtagataagt atgaaagttt aatgaaaaaa aatggttata tgtttgtcaa
    16861 tgaatcacta gtaagtaagg atagaaagta cagagaagac attaatgtta taaaagtacc
    16921 tgttactgaa atagcacaaa atttaggcaa tgaaaaagct gctaacattt gcatgttagg
    16981 tgctatggta aagagtttgg atttatttac aaaagaagaa tttgaaagtg gtatgtgtga
    17041 atactttgaa aatcaaggca aaggtaaatt caattctaaa aatgtagaag catttgaagc
    17101 tggatacgac tacgaatgaa tattttaaat atttaaaata attattgaaa tacttatttt
    17161 tatatgttag tatttaatta ataaaatata gaacgtcgtt ctggacaatg ggacgaagtc
    17221 tttaggtcgg aatacaagaa cggcgttctg tataatagaa ctagggggtg ttaaattgaa
    17281 agttttagta ataaatcctg gtggtacatc cacaaaaatt tctgttttcc aagatgaaaa
    17341 tgaaattttg aaaaaaaata taacccatac aagagaagat ttaaaaaatt tttctaaagt
    17401 atttgatgaa tatgattata gaaaacaact gatagtagat attttaagta gtgagaatca
    17461 tagtataaat tcttttgacg cagtagttgg tcgtggtgga cttatgaagg ctataaaagg
    17521 tggtacctat actgtgagtg aagaaatgat tgaggatatg agaaatgaaa taaatgggga
    17581 acatgcatca aatttaggtg cattattagc taaaactata gctgatgaaa taggagtaca
    17641 atcatttgta gtagacccag tttctgtaga tgaatttgat gatgtatcga ggattacagg
    17701 aatatcagac atagaaaaag caagttggtt acatgcatta aaccataaag ctgtttcaag
    17761 aaaaattgca caaaaactag ggggaaaata cgaggattat aactttattg tagctcattt
    17821 aggttcagga atatcgattg tagcacataa acaaggcaaa atgattgatg gaagtggtgg
    17881 taggtctgat ggtccatttt ctccagaaag gagtggtgga ttacttgcat atcctcttat
    17941 agaattgtgt tactccggaa aatatactca tgatgaaatg gtcgcaaaag taagttcgat
    18001 tgggggaatg tacgattatt taggaacaaa ggacatgata gaaattgaaa acagaatcaa
    18061 ggagggtgat gaaaaggcaa aacttataat ggatgcattt atttatcaag tagcaaaaga
    18121 aatttctatg tatggagctt cactttcagg gaatgtagat agagttatct tgacaggagg
    18181 gatatctcac tcagaaatgg tagtcaaagg agtagcagaa aaagtttcat atctagctcc
    18241 aatagagata gtaccaggag agatggaaat ggaggcactt gctctagggg cacttagagt
    18301 acttaatgga gaagaaatag caaaaactta ttaatattta ggagaaagag tatgttaaaa
    18361 gattttaata atttattaga agaggctaaa aagcttgaac ctgttattgt aagtgttgct
    18421 gttgcagaag acaaagaagt attaaaggct gtgaaatcag caacagattt aaacattata
    18481 aagccaattc ttgtaggaga caaggataaa atagaaagta tatcaagaga aattggatta
    18541 aaaggtcatg aaataatagg ttgtcaaaat cccgatgaat cagttaaaaa agctgtagag
    18601 atagttaaaa ataaggaagc taaagttctt atgaagggat tagtaaatac aagtgtctat
    18661 atgagagcta tattaaataa agaaacgggt cttagaacag gaagattatt aagtttgctt
    18721 gcagtatatg aattacctca atatcataaa cttctttatt gtactgatag tggaataaat
    18781 gtatcgccaa acttaaatca aaaaaaagat ataatgacaa atgcacttct tactatgaaa
    18841 agtataggca tggaaaatcc taaaactgcg atacttactg caaatgaaat ggtagaccct
    18901 aagatacaat ctacagtgga tgccaaccta cttgtagaga tggaaaaatc gggagaaata
    18961 ccaaagtgta tagtggaagg tccaatagca tttgatgtag catttgatgc acatgcagca
    19021 gaacataaag gtattaaaag taatattgct ggtgatgttg atttattagt atttccaaat
    19081 atagaagcag ggaatatatt aggaaagtca tggcttagat ttaataaagc taagtgggca
    19141 ggtatagttt taggagcaac aaatccgata attttaggtt cacgttctga cacagctgaa
    19201 ataaaaataa attcaatagc attagcgtgc ctagcatcac aataagaaat tattattaag
    19261 gaggaaaaag tattatgaaa tatgcaaaaa gaatggatat ggtaaaagct tcagctataa
    19321 gagattctca aaagaaaata gctcagaagg tagcaagtgg tggaactgtt atatcttttg
    19381 ctgcagggct tcctgaccca aatttatttc caatagaaga gataagtgaa ataactcaaa
    19441 aagtacttaa tgaaagggga aaatttgccc tagcatatgg acctactaaa ggtgaagatg
    19501 agttgttaga tctattagta aaaagaatga aagaagaaga aaatattgat acaaaagcag
    19561 aaaatattat aataactact gggtcacaac aagggatagc attaagtgct atgattttat
    19621 tagaaaaggg agatgtagta gttacagaaa atccaagtta cttaggtgca ataaatgcat
    19681 ttagacctta tgaatgtgat tttttaggag tagatacaga tgaagaagga atggtaacag
    19741 atgatttaga caaattatta tctgaaaatt ctaacataaa ggtgatatat gtaataccaa
    19801 catttcaaaa ccctacagga aaaacatggt ctttacaaag aagaaaagat tttatggaag
    19861 tcgtgaataa atatgatgtc atagtaatag aggataatcc ttatggagaa ataagattta
    19921 caccagaacc tctacctaca cttaaatctc ttgatactaa agataaagta ttgtaccttg
    19981 ggtcattttc aaaggtatta tgtccaggat ttagagttgc ttggatgtgt ggtaatgtag
    20041 acatcataga taaggcagaa cttttaaaac aaggtataga tttacagtca aatcaatttt
    20101 cgcaagtaca agtaatagaa tttttaaaga aatacaactt aaatgaacat atagaaaaaa
    20161 taagagcaga gtataagaaa agatgtctgc taatgttaga tgctatgaag aaacatttcc
    20221 cagaggaagc taaatataca gaaccagatg gaggaatgtt tatatgggtt gaacttcctg
    20281 aaagtataaa tgtagatgat ttattagata aagccattga tgctggagtt gcttatgttt
    20341 ctggagagtc cttctttgca aataatggtc caaaaaatac tatgagactt aactatacaa
    20401 caatgtcaga agaacaaatc gttaaaggtg ttgagatact tgcagaagtt ataaaaaaag
    20461 aactcaatta tgcttgtgaa taaatataaa aagtgagggt ttaaatatgg gagaaaaaga
    20521 aaacggtata aagtttggaa agttaatgtt tcaacttgtt attatagcaa tatcttggga
    20581 attagtttat attataccat ttatccaata tacattatat gatccaatcc taaaagcatt
    20641 agaatgtaca aacactcaac taggttttct acttacaatt tatgggttag gtaatatatt
    20701 tggagctcct ataggaggct ggttagcaga tagatttgat tatagaaaga taattttagg
    20761 ttctgtattt cttaatggag tagtatcatt tttatttgca tttaatatga actatccatt
    20821 tgcagttgct acttgggtag gttgtgcaat cacatcatta gttatgaact atccttctat
    20881 ggtaaaaata cttagagtta taggaaaaga taatcaaggt aaggtttatg gatttaatga
    20941 agctatggtt ggagtttctg gagtagtaat gggagcaata ttcttatata tttatacatg
    21001 ctttgcaact cctacattgg gtatgagatg ggtaatgata tctttaggaa tattatcaat
    21061 agcaatgtgt ccagtattat ggtttgtaat taaagatgta gatattaaag aagaaagaga
    21121 agaagcaggt gaaaaaatgt ctgctggtga ttttataaca gtactaaaat caccaaatac
    21181 ttggctagta ggaataagta tattctgtgt atattcattc acagttacaa tgtcttattt
    21241 tacaccatat ataacatctg tacttggagg ttctgtagca ttatctggtg cattagcaat
    21301 aataagacag catggtctaa aattatttgg agcaccattt ggaggatatt gtgcagacaa
    21361 attaaaatct cctacaaaag tacttttacc aatatatgta tttggtatag cagtaatagt
    21421 tttattccta gtattacctg catcaactcc aatgactata ttcatagctt taacatttgt
    21481 agtaggtata ttaggttaca tgggaaaagg tatatattat gcagtacaag atgaagtaaa
    21541 agttccagtt aaatattcag caactactat aggtatagct gcagctttag gattttcacc
    21601 agatgtattc caatttgcat taataggaca ctggatagat acttatggca ataaaggtta
    21661 cacatataca tttatattcc aaatagcaat tctagttatt ggaatactat cttgtttata
    21721 tatattaaag gttaaaaaga gaagagcaag taaattagaa gcttaaatat tcaatttggt
    21781 aattaagtat atataaatac aatacttatt ttgatgaatg tgattgtata aataacataa
    21841 aaaatatcaa tagattaagt tttataataa tgtttagaga ataaagtata aaaatcaaaa
    21901 tgttatcaag ttttctaggt tttaacataa agttacccct gaaggtgtaa aattgagtgg
    21961 caattattat ctattttctt acctacataa acctatttaa tttaaattct aagagatttt
    22021 caaaatagat ggtagttgta ctcaatttta aacctcataa taaatacgaa aatttaaaat
    22081 ctaactagat actatttaat tatatattaa taaatatata ttgtactatt tatataataa
    22141 taatttcagg cttttcatta tatgtattcc aatttatatt gtttgattat tattcaa
//
LOCUS       DAGSHT010000044        22142 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGSHT010000044 DAGSHT010000000
VERSION     DAGSHT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.045"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            453..1523
                     /locus_tag="MJB72_001530"
     CDS             453..1523
                     /locus_tag="MJB72_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262649.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1507..2376
                     /locus_tag="MJB72_001531"
     CDS             1507..2376
                     /locus_tag="MJB72_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBY8262650.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2420..3280
                     /locus_tag="MJB72_001532"
     CDS             2420..3280
                     /locus_tag="MJB72_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBY8262651.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3311..4615
                     /locus_tag="MJB72_001533"
     CDS             3311..4615
                     /locus_tag="MJB72_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8262652.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4678..5940
                     /locus_tag="MJB72_001534"
     CDS             4678..5940
                     /locus_tag="MJB72_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBY8262653.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEIVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6127..8097
                     /gene="uvrB"
                     /locus_tag="MJB72_001535"
     CDS             6127..8097
                     /gene="uvrB"
                     /locus_tag="MJB72_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBY8262654.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     IDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8116..10941
                     /gene="uvrA"
                     /locus_tag="MJB72_001536"
     CDS             8116..10941
                     /gene="uvrA"
                     /locus_tag="MJB72_001536"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY8262655.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11200..13017
                     /gene="uvrC"
                     /locus_tag="MJB72_001537"
     CDS             11200..13017
                     /gene="uvrC"
                     /locus_tag="MJB72_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBY8262656.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13017..13946
                     /locus_tag="MJB72_001538"
     CDS             13017..13946
                     /locus_tag="MJB72_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBY8262657.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14160..15956
                     /locus_tag="MJB72_001539"
     CDS             14160..15956
                     /locus_tag="MJB72_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBY8262658.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENLQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16096..17877)
                     /locus_tag="MJB72_001540"
     CDS             complement(16096..17877)
                     /locus_tag="MJB72_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBY8262659.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17900..19786)
                     /locus_tag="MJB72_001541"
     CDS             complement(17900..19786)
                     /locus_tag="MJB72_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBY8262660.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19803..20300)
                     /locus_tag="MJB72_001542"
     CDS             complement(19803..20300)
                     /locus_tag="MJB72_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBY8262661.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20618..22099)
                     /gene="cls"
                     /locus_tag="MJB72_001543"
     CDS             complement(20618..22099)
                     /gene="cls"
                     /locus_tag="MJB72_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBY8262662.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8517 a         2961 c         3697 g         6967 t
ORIGIN      
        1 ttttttttgg gtaaatttat aaaaattaaa aaagttttat tttttatgtt taaaccttgt
       61 aaatttggga cataatataa ttgtaaaact taatatagca aaaataaatt actcaatctc
      121 ccccacttaa agacctctat cccccataga ggtctttttt attttcaaac caaattatat
      181 aaaaaataaa tagttttata tgactcttca taaattaaaa tcacattgtg atagcaaacc
      241 atgaatatga aaaatataga aaattatagc taacataagc tgtatatcat cttaacttca
      301 gtatatttaa caaaaaatgt ttataattta acctttattt taataagttt actatatatt
      361 cactttgtct taatatatcc tttacaaatt aagagtatta ttaatctata agtaaatcgt
      421 agaattaaat taaatatttt gggaggataa aaatggcaga tataagacta gaaaatgtaa
      481 caaaatctta taataaaaaa aatacagtta tagaaaattt aaatcttact atagaagatg
      541 gttcttttac agtattggta ggcccaagtg gatgtggtaa atctactacg ttaagaatga
      601 ttgcaggtct tgaagatata acttcaggta ggttgaagat tgaagaaaat gatgttacaa
      661 aaacggatgc tagtaaaagg gatatagcta tggtatttca aaattatgca ttatatccac
      721 atatgactgt tagagaaaac atagaatttg gtcttaaaaa caaaaaagta gaaaaagaaa
      781 aaagagaaaa acttataaca gaagttattg atgtagtagg attgagagaa tatttaaatg
      841 tgaaaccagg caacttatct ggaggtcaaa gacaaagagt ggcacttgct agagctatgg
      901 ttaaaaatcc aaaggtattt ttaatggatg aaccattgtc aaatttagat gctaaattaa
      961 gaaatcaaat gagaacagaa ttaatacaat tacatcaaaa actaaagtct acatttgttt
     1021 atgtaactca tgaccaggtt gaggcaatgt cgatggcaga taagatagtt ataatgaaca
     1081 aaggaaagat aatgcaaata ggttctccaa agaagatata tcaaaatcct aaaaatttat
     1141 ttgtagctca atttataggg agtccttgta tgaacatact agatataaaa aatgatatta
     1201 aatttggatt cagaccagaa aaagcaacac tagagagtat aaacatagat gggaaaaatg
     1261 atgatgtatt tagtctattt ggaaaagttg taactaaaga agtgttggga aatgaaacta
     1321 tatattgttt atctgttctt aatagagaga taagagtcaa aacggaaaat gatatatttg
     1381 atattggaag aactttaaga ataagtgtat tagaaaaaga tttatattat tttgataaaa
     1441 atggagaaag aatagaagat gtaaaagtat gtaaaagtat gtattctaaa attaaaggag
     1501 atatttatga aaaagcaatt taataaagca tatttatata taacacctgc tattatagga
     1561 actttattgt ttatagtcta tccaataatt aagacgatat ctctaagctt caaatctggt
     1621 agtcttctta gttcaacctt tgaaaatgta ggcttaaaca attatacaga acttttaaag
     1681 aatccagagt ttatacaaac tataacgaac actgcaattt atactttttt tatggtgact
     1741 atatctatat cactagccat agtacttgct gtttggctaa ataaaaattc tataatacac
     1801 aatttaacgc aaagtataat ctttacacca catgtaattt cactagtatc tgtagctgta
     1861 ttgtggcttt ggattatgga gccacaatat ggctttttaa actggatact atcgacatta
     1921 catttaccaa catcaaagtg gctttcaagt gaaagttcag ctttaatgtc actaattcta
     1981 ataggaatat ggaagactct tggatataat atcttaatag tgttatcagg tcttaaatcc
     2041 ataccaaact atatatatga agcatctaaa ttagataatg caaacaaaat aacgaatttc
     2101 tttaaagtga cattgccact attatcacca acaatatttt ttcttatgat aacaacaaca
     2161 actgcatctt ttcaagtatt tgatgagata agtataatga cacaaggtgg gccagtgggt
     2221 tcaactaaca tgttatctta ttatatatat gagtctggat ttatatacta tgacatagga
     2281 caagcgagtg cagcgagtat gtttttgtta gctatagtta tgattgctac atatgctaac
     2341 tttagactct tatcaaagaa agttcattat caataaagaa aaagtagata ttataaggtt
     2401 tatccaaaag gaggagacaa tggatattat acaacctatc aaaaaagaca aagttgatat
     2461 aaaaaaaata gcatcaaaat caatagaaat attaattcta ttacttttat ctgcaatatt
     2521 tatattccct tttatatgga tgctatctac atcctttaaa aatccacaag aaatgatgca
     2581 attaccgccc actattatac caaatgatat agtgttagaa aattatagta cagcatggca
     2641 ttcaggtcca ttttttagat atttgctaaa cagtgtattt gtaacaggaa gtgttttaat
     2701 tgtacaattt ttagttatgg ttccagcagc atatgcattt tctaagatta aatttaaagg
     2761 tgaaaaaatg ctatttgggc ttttgcttgt aggacttatg attccaccac aagtaacttt
     2821 tctaccagtc tatatgatga tgagtaagtt tggatttata aatacttatg ttccacttat
     2881 aattccattt atgacatctt catttgggat atttctgctt agacagaatt ttatgcagat
     2941 ttcagatgaa attattgaag cagctaaact agataatgct tctgacttaa aaataatgtt
     3001 tagaataatg gttcctatgg caaaaccagc agtaataaca tttatattat ttaattttat
     3061 ataccattgg aataattatt tctggccctt agtaatgaca aatacagatg caataagaac
     3121 tttaccaata ggagttgcgc tccttaaatc ttcagaagga attactgcat ggaatgtaat
     3181 aatggcagga aatataattc taattttacc aataattctt gtttatatat ttgcgaataa
     3241 aaaagtaaaa gaagctttta tgtattcagg aataaaataa aaaattaaag actaaatttg
     3301 gaggatgaaa atgaagctta aaagaaaatt agcaacagga ttagcaataa cattattatt
     3361 aggttcttta gttgggtgtt caaattctaa tgatgcaaat gcatctaaag gagaaaagac
     3421 tgatagcaat attacgaaga tagatttctg ggctccttta ggtggaacta atggagaaac
     3481 tgctcaaaaa atggttgacc aatttaatag tgaacataag gatgtacaag taaatatgtt
     3541 aaaacagaaa gattactatg aaaatgcaac aaaactacag gcagccttaa catctaaaga
     3601 ccaaccagat gtaacattat tagaaataac tcaaacaggt acattcgcat cagcaggtgc
     3661 tttagtagat atgagtaaat attttgataa aacatatcaa gaaagattct tttctggtct
     3721 tttaacaaat tcatattatg aggataagtt tgttggtatg ccatttaata gaagtacacc
     3781 aatattatac ataaataaag atatggcaac taaagcaggt cttgaccctt caggtccaaa
     3841 atcatgggaa gagcttaagg attatgctag taaaatgaca aataaatcag aagatactta
     3901 tggttttgaa acgccaatag atatttggtt ctatgaagct atggtaatgc aaagtggagg
     3961 agaaataact aatggtaaaa aagtagcatt taataatgag gcaggacaag caccagttaa
     4021 gttttggcaa gatatgatga aagctggaat aatgaaaatg cctcctggag aagattataa
     4081 tgcctgggac gtagcaaaac aagattttgt aaatggtaaa gtaggtatga tatttacatc
     4141 aacagcagat ttagcaggac ttatgcaaca aacagaaggt aagtttgaaa tatcaacagc
     4201 attcttacca aaaaatcaaa aatacgcaac tcctacaggt ggagctaatc tagttatgct
     4261 tgaaggtgga acagataaag aaaaagaagc aagtgcagaa tttatagaat ggatgacaca
     4321 aacagataaa atcgttcagt ttagttcatc aacaggatac ttaccaacaa ctgaagatgc
     4381 aacaaactca gaaaagttac aaacattata taaagaaaaa cctcaatata aggttgcaac
     4441 tgaccaatta caatatgctg ttgcacttcc aatgttaaat ggatacaagg aagcaactga
     4501 taagctaatg gatgagatta aaaaggggct tactgattta aatgcaaatc caaaagatgt
     4561 agtaagcaaa ggaacttctg caatgcaagc agtaatagat aaaacaaata aataattata
     4621 tagacttgta agttgattaa aagtattcaa taatatataa atgaagaggt aatgtaaatg
     4681 acaaaggtta tagtattatc agtagattct ttatttgaaa aagacttgga atttgtaaaa
     4741 aatctaccaa actttaaatc tatactagaa aattgctcca tagtaaaaaa tataaattgt
     4801 gtttatccca ctctgacata tccgtgtcat actagtatgg ttacaggagt atatcctaaa
     4861 aaacatggta tatatcataa tgaaaaattt gacccaaata aagaaaataa agactggtac
     4921 tggtactcaa aggacataaa agcaaaaact attatagatg tagcaaaaga aaataatcta
     4981 actacctcta gtgtattatg gcctgtaatg ggagcaaatc caaatataga ttataatatt
     5041 gcagaaatat gggctcctag tagagaagat gacccaagag aaacttttga acgaagttcc
     5101 agtaaggtta taatggacgg tatttacaat agacattgtc attatatcga ttggaaatta
     5161 gaacccaaca tggatttatt tggagtaaac tgttcagtag atataataaa ggaatataaa
     5221 ccagatttaa tgctaataca tttagctaca ttggaccata cacgacataa ttatggatta
     5281 tttaatgacg aagtagataa agcattaaaa atgaatgata aatggctagg agatattatt
     5341 caggctacaa aggatgcagg tacatacaaa gatacaaatt ttataatact tggggaccat
     5401 ggtcatctta gagttgataa agttataaat ccaaatgtct tgcttaaatc aaatggattt
     5461 ataaaagttg aaaatggaga agttaaggat tttgatgcct atgtaaattc ttctggaata
     5521 tcagctcaaa taatagtaaa taacttagat aggttaacag aattaagaga actgcttgaa
     5581 gaatttaaag aagaattatt catagagaat atttttacca aagaggaagc atctaacctt
     5641 ggtcttgaag gtgattttga aatagtgatt gaaggtcttg aaggaatttc atttggaaat
     5701 gatttcgaag gaagtattat aaaagattca gatataaaag attacaagtt tgctgttgcg
     5761 acacatggtc atttacctac taaaggtaat agacctccat ttatagcttt tggaccaaat
     5821 attaaaggtg gtgtggttat agaagaagga gacctgagag cacatgcagc aactatatta
     5881 aaaatgttta atttaaaatt agatggagtt gaaaaagaag catttgattt tattatgtaa
     5941 gttgaataaa gtatttattt tccatgaatt atagacttct attgcaaaga tagaagtctt
     6001 tttttatgtt ttagatttaa gtaaaattta aatgggtaaa atatttaaaa taacttaaac
     6061 aattttataa aggtatgata taataataca tgtcaggaac taatgttcga aaaaaggagt
     6121 gatactatgg attttaaaat aaaatcagac ttcaaaccta caggtgacca accagaagct
     6181 attaaatcaa tagtggattc tataaataga aatgagaaat tttcgacatt attaggtgta
     6241 acgggttcag gaaaaacatt cacaatggca aatatcattc aacaagttaa gaaacctacc
     6301 ctaataatgg ctcataataa aactctagca gcacaacttt acagtgagtt taaagaattt
     6361 ttcccagaca atgcagttga atactttgta agttactatg attattatca accagaagca
     6421 tatgtagctc acagtgacac ttatatagaa aaagatgcaa gcataaatga tgaaatagat
     6481 aaacttcgac attctgctac agcatctatt ttagagagaa gagatactat aataatatca
     6541 tctgtatctt gtatatatgg acttggtgac ccaaaagact ataaagaact tatgttatct
     6601 ataagaccag gtatgcaaag agacagagat gatgtaataa aaaggttaat agagatacaa
     6661 tacgaaagaa atgacataaa ctttacacga ggtaccttta gagttagagg agatatacta
     6721 gaaatattcc cagctagtaa tgatgaaaaa gcaataagaa tagaattttt tggtgatgag
     6781 gtagatagaa taacagaaat tgattatgta acagggaaga ttgttggtac tagaaatcat
     6841 gttgttatat tcccagcatc tcactatgta acaacgccag aaagaattga aaaggcaatt
     6901 gtagaaatag aaaacgagct tcaagaacaa attaaatttt tcaaggaaaa tgataggcta
     6961 ttagaagcac aaagaattga acaaagaact aagtatgata tagaaatgtt aaaggaaata
     7021 ggattttgtc aggggataga gaactattct agacatatta caggaaggtc agaaggagaa
     7081 agaccatata ctttaatgga tttcttccca gatgattatt tgataattat agatgaggca
     7141 catgtcacta taccacaagt aagaggtatg tatgcaggag acaggtctag aaaaacatca
     7201 cttatagaga atggttttag attaccttct gcacttgaca atagaccatt aaattttcaa
     7261 gaatttgaag gaaatataaa tcaaatgttg tttgtaagtg ctacaccagg accttatgaa
     7321 attcaacact cagagacaat agcagaacag ataataagac caactggcct tttagaccca
     7381 attgtagaag taagacctat aaataatcaa atagatgatt tggtaggaga aataactaaa
     7441 actatagaaa aaaatgaaag agtgcttata accactttaa ctaaaaaaat gagtgaggac
     7501 ttgactaact atcttaaaga aatagggata aaagttaaat atttacactc agatatagtt
     7561 acattagaaa gaacagaaat aataagagat ttaagattag ggaagtttga tgtccttgta
     7621 ggaataaact tacttagaga aggattggat ataccagaag tttcacttat agcaatttta
     7681 gatgcagaca aagaaggttt cttacgttcc gaaacagctt taatacagac tattggacgt
     7741 gcagctagaa atgaaaatgg aagagtaatt atgtatgcag acaggataac tgactcaatg
     7801 caaaatgcta ttgatgaaac aaagagaaga agagatatac aaaatttata taatgaagag
     7861 cacaatatca ttcctaaaac tatacagaaa aatataagag atagtataga agctactaag
     7921 gttgcagagg aagaagttgt atatggaata tctgatacag atgataaaga cgaaataaga
     7981 gctaatatag ataaattaaa atcagagatg atggaagctg ctcaaaactt acaatttgaa
     8041 agagcagcag aacttagaga taaagtgaaa caattagaag aaaaattgga aaaatagaaa
     8101 ggtaaaaaca acattatgga agataaaatt ataataagag gagcaaaaga acataattta
     8161 aaaaatatag atttagaatt accaagagat aaatttattg tttttacagg tttgtcaggg
     8221 tctgggaagt cttctttggc atttgataca atatatgcag aaggacagag aagatatgta
     8281 gaaagtctat ctgcttatgc aagacaattt ttaggtcaga tggaaaagcc aaatgtagaa
     8341 tatatagagg gtttatcacc agctatctca attgaccaga agactacctc aaaaaaccca
     8401 cgttctacgg ttggaacagt aacagaaata tatgattatt tgagactatt atttgcaaga
     8461 gtaggagatg tgtattgccc tacatgtggt gaaaaaataa gtcagatgac tattcaagaa
     8521 atagttgata aaatgctaga ttttccagat agaacgaaat tacaaattct ttcaccaatt
     8581 gttagaggac aaaaaggtac acataaaaaa gtctttgata atatcaaaaa ggaaggattt
     8641 gtaagagtta gagttaatgg tgaaaactat gaagttagtg atgatataat cttaaataaa
     8701 aatcaaaaac ataatataga agttgtagtg gacagaatag taattaaaga tggaatagaa
     8761 tcaagactag cagattcaat tgaaactgca gttaagctat ctgatggtct tgtaatagcc
     8821 caaataatag atggagaaga aattcttttt tcaactaaat ttgcatgccc agaacatgga
     8881 attggcatag aagaattatc tccaagaatg ttttcattta atgctccctt tggtgcttgt
     8941 gatgtttgta atggtcttgg ggaaagtaaa gaagttgacc cagaattagt agtaccaaac
     9001 aaagatttat caataaaaca aggtgcagtg gcagcatggg gttcaacagg tgttaatgat
     9061 gacacttatt acagcaaaat gataaaaagt ttagcagagc attttaatgt atctttgaca
     9121 acaccgttta aagatttacc agatgatttt gtggaggaat tactttatgg taaagataat
     9181 ataattgtag agtttactta tgagtcaaaa tttggaggaa ctagaaatta taaatcatac
     9241 tttgaaggtg taatagtaaa cttagagaga agatatagag aaactaattc agattacatg
     9301 agagataaga ttgaagaata catggcagag agaccatgtc caaagtgtaa agggatgaga
     9361 cttaaaaagg aagttttatc tgttttagtt gatggaaaaa atataatgga agttacaaac
     9421 ttatctgtaa atgaattgat tgactttatg gaaaatataa atcttacaga aaaacagaga
     9481 tttatagctc atgagattat aaaagagata aaaggaagag ctatgttctt aagagatgta
     9541 ggtcttgatt atttaaattt atcaagaaaa gctggaactt tatcaggagg agaagcgcaa
     9601 cgtataagat tagctactca aataggttct gcattggttg gagttttata tgtacttgat
     9661 gagccaagta tagggcttca ccaaagagat aatgataggc ttataactac tcttagacat
     9721 ctaacagatt taggaaatac cttaatcgtt gtagagcatg atgaagacac aatgagagaa
     9781 gctgattatg ttatagacat tggtccaggt gcaggtatac atggtggtga aatagttgca
     9841 cagggtactt tagatgaaat aattgaaaat gagaactcaa taacgggtca atatttaagt
     9901 ggtaaaaaac aaatattgct tcctgagact actagagaag gaaatggaag ttttatagaa
     9961 atagttaaag catcagaaaa taatctgaaa aatattgatg taaagttccc attaggtaag
    10021 tttacatgta taacaggtgt ttcaggttcc ggaaaaagta cattaataaa cgatatactt
    10081 tacaaaggtg ttgcaagtaa agtgaataga cttaagcaaa gagctggtaa gcataaggag
    10141 ataaaaggaa ttgaaagtat agataaagtt ataaatatag accaaagtcc tattggaaga
    10201 acacctcgtt caaatcctgc tacttataca ggtgtatttg attttataag agatattttt
    10261 gcatctacta atgaagctaa agcaagaggt tataaaaaag gtagatttag ctttaatgta
    10321 aaaggtggta gatgtgaagc ttgtaagggt gatggaataa taaaaataga aatgcatttc
    10381 ttaccagatg tatatgttcc ttgtgaaatt tgtaaaggtg aaagatacaa tagagaaaca
    10441 ttgcaagtaa aatacaaaga taaaactata tctgatatat tagatatgaa tgtggaagaa
    10501 gctgttgagt ttttcgaaaa tataccaaat ataaaaagaa aattagaaac tttaatggat
    10561 gtaggacttt catatattaa attaggacag ccttcaactc aattatcagg aggagaagca
    10621 caacgtataa aattagctgc tgaacttagt aaaagaccaa caggaaagac tttgtatata
    10681 ttagatgaac ctactactgg acttcatatg gcagatgtag acaagctaat ccaagtatta
    10741 caaagactag cagatacagg aaatacaatt atagtcatag aacataattt agatgttata
    10801 aagacttgtg attatgtaat agacttagga ccagaaggcg gagataaggg tggaactata
    10861 gttgcaactg gaacaccaaa agaagtatct aaagttgatg gttcttatac aggtcaattc
    10921 ttaaagaaat atttttctta agttatataa attgtgaaaa attttagagc agatatacga
    10981 taaactgttt tgttataagt aaattagtca aatataagtt gtaaatagta atacaaagta
    11041 aagtccatat tactcttcat ggttaaaatt gttttcaaat caattataga agggaatatg
    11101 ggctttaact tgtttaaagc actggaaatt gtataacttt ttaaaattca tattttaggg
    11161 tataatttta gtataaaatt taatgttttg gagggcgaga tgtttgatat acaagagcat
    11221 ttaaaaaaat taccttctga gcccggagta tatttaatga aagacaaata cgaccatata
    11281 atatatgtgg gaaaagccat atctctaaaa aatagggtaa ggcagtattt tcaatcttca
    11341 aaaaaccata catctaaagt aaaatctatg gttaagaata tatataagtt tgagtatatt
    11401 ataacggatt cagagttaga ggcattaata cttgaatgta atttaataaa aagatacaga
    11461 ccaaagtata atgtagtgct tagagatgat aaaacttatc catatataaa agtaactacg
    11521 aatgaagatt atccaagaat cttaaaagtt agaagagttc ttaaagataa ggcaaagtat
    11581 tttgggccct ataccaatat aactgctgtg aatgatacct tggagcttat aagtagtacg
    11641 tatccaataa gaagctgtaa aatagatata gataaagcaa taaagaataa gacaagacct
    11701 tgtttaaatc tacacataaa taaatgttta ggaccttgta caggaaatgt ttctaaagaa
    11761 gagtatggaa aaatgataga agagattata atgtgtttat ctggtaaaga agaaaaattg
    11821 atggaacttt taaaggaaaa aatgaatgag agttctatga actttagatt tgaagaggct
    11881 gctgtatata gggataagat aaagagcctt gaagagatga tacagaaaca aaaaatagat
    11941 gctactgtaa gtgacttaaa ccaagatgtg gttgcaatgg caagagctca taatgaagct
    12001 tgtgtccagg tattttttat aagaaatggt aagattgtag gtagggaaca ttttatatta
    12061 gaaggggtaa tggatagtcc aagagcttct atattaagtt catttgtaaa acaattttat
    12121 aatgaacaag agtatatacc taaagaatta attatagaag atgagataga agatagttct
    12181 atattagaag aatggctgtc atctaaaaag gggcaaaaag taacaataag agttcctcaa
    12241 aagggagaga agaaaagctt agttgaaatg gttaggaaaa atgctgtaga atatcttgaa
    12301 aaattttctg atatgaataa aagaaaatat gaaaaaagta ttggagcatt ggaagaatta
    12361 aagcaaatat taaatctaga aaagttgcca ataagaatag aggcttatga tatatcaaat
    12421 atacaaggtg tagattcgat tggttctatg gtagtgtata cgaatgctaa aaaagataag
    12481 aaggaatata ggagatataa aataaagaca gttattggac ctaatgatta tgattctatg
    12541 gctgaaattg tagatagaag attaaaacat ggaaatttac ctgacttgat attgttggat
    12601 ggtggaaaag gacaagttag tgcagtaaaa aaagtactag aactcaatga tgtagatata
    12661 cctctttggg gaatgtacaa agatgataag catagaacta aaggattgat atgcaaagaa
    12721 aaagaaatag aactagataa aacaactaat ttatatagat ttattgctag tatacaagaa
    12781 gaagttcata attatgcaat aacatatcat agaagtctaa gaaataaagc tttgacaaaa
    12841 tctatcttag atgatataca aggaataggt gaaaaaagaa agaaatcatt attaaatcac
    12901 tttaaagatg tagatgctat aaaaaaagct actatggaag aacttttaga agtagatgga
    12961 atgaataaaa gcattgcaga taatgtttat aattttttta gaaaggaaga aaattaatgg
    13021 aaactagcaa taaagtatca ataagagaga taataaaaga gttgaagtta aaagtagtat
    13081 acatgcctga tgatgtagat tattatgtct actctcaaga tataaataga ccaggattac
    13141 agtttgcagg atattttgat tattttgcat atgaaagaat acaaatagtt ggaaagacag
    13201 aatataattt ttttggatta atggatagtc atattcgaga agaagtacta gatagatttt
    13261 tctcatatga aataccagcg ttaataattt ctagagactt ggaaatcaag ccagatgtaa
    13321 ttgaaaaggc taaaaaatat aaaagaatac ttttgagcac aaatagaaat accactagac
    13381 ttataaatag actgtcaaac tatttggata ataagttagc accacacaca acaattcacg
    13441 gggtgctagt agatatttat ggtataggtg tactgataaa aggtgaaagt agtataggaa
    13501 agtcggaaac agcattagaa ctgattcaaa ggggtaatag attagttgca gatgatgctg
    13561 tagaaataag aaaattagat gaaagtatgc ttgtgggtca gtcacctgaa ctaataagac
    13621 attttcttga gataagagga ataggaataa tagatgttag aagtttatat ggagtagggg
    13681 caatcaaaaa ttctaaaatg atagatttag tagttcattt agaagcttgg gatgaaacta
    13741 agtattatga tagactagga ctggataaag aatatgaaga aatacttggt gttaatgtag
    13801 agaaattagt tgtacctgtt aaacctggaa gaaatactgc tatgatatta gaagtagcag
    13861 caatgaattt tagacaaaga ggaatgggat acgatgcagc tcaagaattt actaaaaaat
    13921 tatcaaaatt aatcgatact aagtagtact gaataatagt gaactacaca tcatttaatt
    13981 aaatattgat taggattata tcctacacct tataaattaa aatcaagttg tatatatata
    14041 atgtttatta ataagatata ttatataagg ataatttaaa gtacagtgtt agattataat
    14101 aatactagat taattttata tttgagtttc attatataac aagaaagttg gaatttttta
    14161 tgggaataga atattttagt aaacttttag aaagtagtac tgaaaaagaa ttgctcttaa
    14221 gtaaaaagtc aaataatata agtacagcaa gattaatatc ttttttgata gtgatagcag
    14281 ggcttgctat tggattttac aataaaaata tagtaggtat ttttgttgga gttttatcaa
    14341 taataatatt tatagcactc ttagttattc atagtaaagt aaaagaagaa gaaacatatt
    14401 ttaaaagcaa gagcgaagta ttaaataagt atattaagag atttggagat ggttggaagg
    14461 aatttaaaat agatggaaaa gaatacttaa aagatgaaaa cttgcaagca aaagatttag
    14521 acttatttgg aagagcatca ttgtatcaat atatctgtgt agctaataca tcttatggga
    14581 aaaatttttt agccaaatat ttgtggaatg aaaatccaga tgagaatgta attttagaaa
    14641 gacagagagc tataaaagaa cttttatcaa aagatgattt ttctattcat attcaaacac
    14701 tcagtaatat aattggtaaa gagcaaaaaa ataattcaga tagtagtata gaatcgttta
    14761 tagagtatgg agaaaataag aatgtgtata taccaaaatg gatgcatatt tttacttggg
    14821 gtttaccaac tgcaacaatt ttatctttta tattttgtat gttaggattt ttacctgttt
    14881 tacctgtttt tttacttttt ataatccagt tagggtttag tggttttgga tatccaagac
    14941 ttatgcaaac tcttgctcca ttattttcat ttagtagaag tatacaagta tatgaaaaaa
    15001 tgtttgaggt tttagaaaaa gagacgtttg agagtgcata tttgaaagaa ttacaagata
    15061 aactttctaa gggaagtgga gtttcaagag gtgttaaaca attaaattca ataggaaatg
    15121 ctgtaaacct aaggtataat cagatactat atataattgc ctgtggtgtt ttgatgtgga
    15181 actatcattg tgcagaagct ttggagcgat ggaaaggtat atatggaaat caaattagag
    15241 attggtttga atctattgga gaatttgaag ctctaataag tttaacagtt atttctcatg
    15301 taaaggaaaa tacatgtttt ccagtaataa aatatgaaga tactccaaga ttgaaagttg
    15361 aagaggtata ccacccatta atagcagaaa aaagtgtcat agcaaattcg atacaattaa
    15421 attctcagac ttgtattatc acaggttcaa atatgtctgg aaagacaaca tttttaagaa
    15481 gtatcggtgt aaacttagtg ctggcatatg caggtgctcc agtatgcgct aagagttttg
    15541 atgcgacctg tatggctata tttacttcta tgagaataca agatgatgta agtcaaggaa
    15601 tatcaacttt ctatgctgag attcttagaa taaaatcaat gattcaatat agtttaaaag
    15661 aactgccaat gttggttcta gtagatgaaa tttttaaggg aactaactct gctgatagaa
    15721 ttattggagc aagtgaggca gtaaagaaat tatcaaaacc ctgggttata agtatggtga
    15781 caacacatga ctttgagcta tgtgaccttt caggtagtgg agatgttgaa gttgtaaact
    15841 atcatttctc agagtactat gtagacgata aaattcattt tgactataag ataaaagatg
    15901 gaagatgtaa aactacaaat gcaaaacaac ttatgagaat ggcaggtatt ctttaattaa
    15961 tagataggtt aattaaagaa taagtctatt tagaaatata atttaatttg tgaaattaat
    16021 aaggattgtc ttaaattacg taaaagtatt ttaagacaat ccttttttaa gaataaattt
    16081 tatattttgt tatgactact tagcttcttc acaagcacat tcttgtggac actctccata
    16141 gacacaactc ttatcaaagt aatgagtatg aaggtattta tgggctacat cactatttgg
    16201 ttctccaaag aagtctttat aaatagcttg taaatcagga ttctcatgag atttacgaat
    16261 agccttattt ctatctattt catataacgc atttgcacgt ctatcaacta tttcagtatt
    16321 accatggtga taaggttgac cagcacctcc aacacatcca ccaggacatg ccataacttc
    16381 tatgatatgg tatttacatt ctccacttct gaccttatcc ataatctttc tagcgttttt
    16441 aagtgtattt acaatacaca cattaactgt agttccatca acatctatac ttgcttccct
    16501 aattccttta agacctctaa cattagtgaa gtttacatta tctaaagtct tattagttat
    16561 cttctcatat gaagtacgta gggcagcttc taaaactcca ccacttgctc caaatataga
    16621 tgcagcacct gtagattttc ctagaggatt atcaaaatct tggtcttgaa gattaggtaa
    16681 atcgattgca gcttctttta tcatcttagc taactctcta gtagttatag aaaggtcaac
    16741 atctagtatt ccagattcac ttaattcttc tcttgaagct tcatattttt tagctacaca
    16801 aggcattaca gatacaacat aaagttcttc aggattgatt cctaatattt taggtgcata
    16861 gtagttttta gctatagaac caaacatacc ttgaggagac ttacagctag atgctaagtt
    16921 taacttgtct ggataattgt gctctaagaa gtttacccat gcaggacaac aacttgttaa
    16981 tataggaaga ttttcacctt tttggattct ttctataaat tcagtagctt cttccattat
    17041 agtaaaatct gcaccaaagt ttgtatcaaa tacatgttca aatccaagtg cttttaaagc
    17101 agctaccatt ttccctgtag agattgaccc tggttctaat ccaaactctt caccaatagc
    17161 aactcttaca gcaggagcaa cttgcactac tatagtcttt tcttttttat ttaaaacatc
    17221 ccaaagttta ggtacattat ctacttctgt taatgctcca gttggacaga cagatataca
    17281 ttgaccacag aatgtacact ctgtttcaac catatcagag ttaaagaatg tactaactaa
    17341 agtattaaat ccacgattaa ctccagataa tgctcctact gtttgtatgt cattacatac
    17401 agtctcacat cttctacata atatacactt agaatggtct ttaactaaag atttagtaga
    17461 agtatcttta cctgcaaaag atttagaacc ttgatatctt atttttctta ctcctaaatc
    17521 agcagctata gtctgtaatt cacaatctcc atttttttca cagacaaaac aatcttgtgg
    17581 atggtctgat aacaataatt caacaatagt tctacgagca tttaatgctt tagcactatt
    17641 agtctgaact ttcatacctt cctttataac tgttccacaa gctggaacta atcctctctc
    17701 tgtctcaacc atacaaacac gacaagaagc acaagtgtca agcttatcta tttcattcat
    17761 atgtaaatga caaaggttgt gtatttttat atttattaat ttcgcagcat ctaagataga
    17821 agttccacta ggtgctgaaa cgtgtttgcc attaatagtt aaattaacta aactcattgt
    17881 tattcctcct ccaaataagt tatttcttga ttatagcgtt aaatgtacaa ttatctatac
    17941 aagcaccaca ttttagacac ttagaagtat ctattgtatg ttgttcctta acagaacctg
    18001 ttatacaacc agcaggacac actctagaac attttgtaca acccttacag tcatctgtta
    18061 taaagtaaga aagtaagttc tgacatttac cagcaggaca tttcttatct acaacatgag
    18121 ctatatactc atctttaaaa tattgaagag tacttaaaac agggtttgga gcagctttac
    18181 caagaccaca taatgaagca gtttgtatag tttcagcaag gttttctagg tcatctaaat
    18241 cttgtagagt accattacct tctgtaattt tagttaatat ttctaataat cttgtagtac
    18301 ctatacgaca aggtgtacat ttaccacaag attcttcaac agtaaactca aggaagaact
    18361 tggctatatc taccatacag tcagattcat caagaacaag cataccacca gagcccatca
    18421 ttgaaccgat agaacttaat gaaccaaagt ctataggagt gtctaaatgt tcagttggta
    18481 tacatccacc agatggtcca cctgtttgaa cagccttaaa tttcttacct tcagggattc
    18541 ccccaccaat ttcaaataca atatctctta aagtagtacc cattggaact tcaacaagtc
    18601 ctacattttc tacttttcca ccaagagcaa agactttagt accactagaa tcttcagtac
    18661 ctaagttggc aaaccaatct ccacccttaa gtataattgc aggtatgtta gcaaaagtct
    18721 caacgttatt taagcaagta ggagatttcc atagaccact cttagaagaa ctataagttt
    18781 tcattctagg ttcaccacgt ctaccttcaa ttgaatgcat tagcgctgta ccttcaccac
    18841 aaacgaaagc acctgcacca tattttaatt ctaatttaaa gttaaatcca gtgcctaaga
    18901 tattttcgcc aagtaaaccg tatttttcag cttgagctat ggctactttt aatctttcta
    18961 tagattttgg atattctgct cttatgtata tgtatcctgt atcagaacct atagcgtatc
    19021 cacatattgc catagcttct agtacactat gagggtcacc ttcaagtaca gatctatcca
    19081 taaatgcacc tgggtcacct tcatcagcat tacatacaac aaacttttta tctgttggac
    19141 cagctggatt ttttgaagca gcttcccatt tagagccagt agggaaacct gcaccacctc
    19201 taccacgaag gccagaagtt tttacctctg caataacttc ttgaggagta agagaagtta
    19261 aacatttgcc tagagctaaa taaccatcat tggcaatata ttcttctata ctatctgggt
    19321 ctataagacc acaatttttt aaagctatac gtaactgttt tttgtagaaa gacatttctt
    19381 cttgttcttc aactttttta cttaaagatt cttcctcata taataattcc tcgacaactg
    19441 tatttctaat taagtggctt tgaactatct tttcagcatc agatggctca acttttacgt
    19501 agaaaacgtt atcaggataa acttttacaa taggaccttg agcacagaaa ccaaaacaac
    19561 ctgtcaaacg aacactaact ttatcttgga ttcctgattt ctttatttca ttttctaatt
    19621 cactaactat ttctagactg tttgaagatg tacatccagt atcacaacaa acaagtaatt
    19681 ctcttcttaa tacattttcc ttagaataat tttttcttaa gctaagatta ggtttcagct
    19741 catcagctaa aacttttaat tcatcaaatg aatttacttt acgcattatt tattcacccc
    19801 tcttaacaat tcaattctaa ttctctataa gtatctaata tttcgcttac ttggtcaggt
    19861 ttcactttac cataaacttt accatttaca gtaacaactg gagctaaacc acatgccccc
    19921 aaacatctta aaccttctaa agaaaattta aaatcactag tagtttctcc aacttttatg
    19981 ccaagctgtt tttcaaaagc acttaagatt ttgtcagcac cttttacaaa acatacagtt
    20041 cctagacaga cactgatttt atacttacct acaggttctg tagtaaaata agaatagaat
    20101 gtaacaaccc catatacctt tgcaggagct actccaagtt ttctagcaac atgaagttga
    20161 acttcttttg gaagatatcc aaatatacct tgggcttcat gtagaacttg tattaacgca
    20221 ccttcctttg ttgcaagaga gtcaataaat atatctagtt cgtcaaataa ttgcttgttg
    20281 tgtgaaataa aatcgcacat aacaaatccc ccttttcttt ttaaagttat tatttttcat
    20341 aaaataatta accatgagtt gaccccaaca ttatgtgggt gaacaaaacc aaaacaataa
    20401 agaaagaata atatacaaca atttctttat ttataaaaac aatgcaaaaa agttacctat
    20461 taaaaataaa aactatacta gtctattata atataaaata attcagaata gttaaaaaaa
    20521 ataaaaaaaa tgagctaaat tcaataaaaa aaaaataata catataaaaa actttaaaaa
    20581 ataaaccaaa atgtaaaatt tttataaaat aaaagattta taaaactgct gataatagtc
    20641 ttgaaattga ttccttgatt tttatataag tagacctaga attatatata tctaatgtta
    20701 tttccttaga atttaaaata tcattttcaa atataattct ttgttccaat gctttttttg
    20761 aagagtacat aaatgcatta acttcgaaat taagctcaaa acttctgata tccatattag
    20821 ctgttcctat agagcatata gagtcatcta taactattgt cttagcatgt aaaaatgcat
    20881 tttgaccata agtatagatt ttagctccaa actttaaaag ttctccagca tatgaatatg
    20941 atgcccagta tacaaaggga tggtcaggtt tagaaggaat catcactctc acatcaacac
    21001 cagataaaca agctattttt aaagtatcta taaaagttga gtcaagtatt aaataaggac
    21061 tctgtatgta tatatacttt ctagctttct gaatcatttt aaggtaacca tatttaatct
    21121 catcaagttc tgatatatct ggaccacttg atactatctg aatcccaata ttttcagaag
    21181 gatgattaga atttgtatct tcttctataa agtatttttc taaatctaag tcttctttag
    21241 ttgtatatct ccaatcaagg atgaatctca tatttaggtc aattacgcaa tcaccagtaa
    21301 gcttagtatg tgtatctctc catagaccaa attttggatt tctacctaaa tattcatcac
    21361 caacattata tccacctaca aatcctacct taccatcaat gacaacaatt tttcggtgat
    21421 ttctatagtt taagttaaag tttacaattt tcaagaatga tgggaagaag cttccaactt
    21481 taacaccact ttctttaagt ctggaaagtg tactattttt tagtgttcta ccaccaactg
    21541 catcaaataa aagtcttact tcaacacctt ctttagcttt gtctataaga atgtctatta
    21601 tttcagttcc gattttgtca tctttgaaaa tgtaaaattg tatgtttata tattttttag
    21661 cttttttaag ttcttcaaga agactgttaa aaaattgact agattcagca taaatccaca
    21721 catcgttatt attggtataa tgagcattat ttgaattagc caatgcataa atcatatcct
    21781 tatgttgatg tatatctgaa tcaatttcag atgtagattg gagtttaacc tgagtatcta
    21841 gtatattact tttgattatc ttatcatcct tttctttcaa tctaaacata ttgttttttg
    21901 aaatatttct gccaaaagct atataaagta tgaatccaag agcaggaatc aatactaata
    21961 taagaagcca tgttaaagtt gtatttatgt ttcttttttc tctaaagata aggtttagta
    22021 taaccatgaa attaattata tatattgtag taactgctat ttcatatatt gacatttcga
    22081 aaaatgatat atcaaacata aaattccctc cttcaaatat ttattaaaat aagtaattat
    22141 at
//
LOCUS       DAGSHT010000045        22107 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGSHT010000045 DAGSHT010000000
VERSION     DAGSHT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.046"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(318..509)
                     /locus_tag="MJB72_001544"
     CDS             complement(318..509)
                     /locus_tag="MJB72_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262663.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            729..1775
                     /gene="cobT"
                     /locus_tag="MJB72_001545"
     CDS             729..1775
                     /gene="cobT"
                     /locus_tag="MJB72_001545"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBY8262664.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1807..2367
                     /gene="cobU"
                     /locus_tag="MJB72_001546"
     CDS             1807..2367
                     /gene="cobU"
                     /locus_tag="MJB72_001546"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBY8262665.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            2377..3150
                     /gene="cobS"
                     /locus_tag="MJB72_001547"
     CDS             2377..3150
                     /gene="cobS"
                     /locus_tag="MJB72_001547"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBY8262666.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3170..3784
                     /locus_tag="MJB72_001548"
     CDS             3170..3784
                     /locus_tag="MJB72_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBY8262667.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      3851..4030
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4160..5659
                     /locus_tag="MJB72_001549"
     CDS             4160..5659
                     /locus_tag="MJB72_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBY8262668.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            5698..7074
                     /locus_tag="MJB72_001550"
     CDS             5698..7074
                     /locus_tag="MJB72_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBY8262669.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            7075..8046
                     /locus_tag="MJB72_001551"
     CDS             7075..8046
                     /locus_tag="MJB72_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBY8262670.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQCIIRKVS
                     KNDRQLKFGGFILWLITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            8078..9148
                     /locus_tag="MJB72_001552"
     CDS             8078..9148
                     /locus_tag="MJB72_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY8262671.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9150..10037
                     /locus_tag="MJB72_001553"
     CDS             9150..10037
                     /locus_tag="MJB72_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBY8262672.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            10025..10657
                     /locus_tag="MJB72_001554"
     CDS             10025..10657
                     /locus_tag="MJB72_001554"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBY8262673.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            10666..11892
                     /locus_tag="MJB72_001555"
     CDS             10666..11892
                     /locus_tag="MJB72_001555"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBY8262674.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            11889..12497
                     /locus_tag="MJB72_001556"
     CDS             11889..12497
                     /locus_tag="MJB72_001556"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBY8262675.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            12487..13062
                     /locus_tag="MJB72_001557"
     CDS             12487..13062
                     /locus_tag="MJB72_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBY8262676.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGCAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            13055..13807
                     /locus_tag="MJB72_001558"
     CDS             13055..13807
                     /locus_tag="MJB72_001558"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBY8262677.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            13791..14912
                     /gene="cbiG"
                     /locus_tag="MJB72_001559"
     CDS             13791..14912
                     /gene="cbiG"
                     /locus_tag="MJB72_001559"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBY8262678.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            14909..15634
                     /gene="cobJ"
                     /locus_tag="MJB72_001560"
     CDS             14909..15634
                     /gene="cobJ"
                     /locus_tag="MJB72_001560"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBY8262679.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            15631..16383
                     /gene="cobK"
                     /locus_tag="MJB72_001561"
     CDS             15631..16383
                     /gene="cobK"
                     /locus_tag="MJB72_001561"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBY8262680.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            16402..17880
                     /locus_tag="MJB72_001562"
     CDS             16402..17880
                     /locus_tag="MJB72_001562"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBY8262681.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            17910..18815
                     /gene="hemC"
                     /locus_tag="MJB72_001563"
     CDS             17910..18815
                     /gene="hemC"
                     /locus_tag="MJB72_001563"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBY8262682.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPIAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            18805..20304
                     /gene="cobA"
                     /locus_tag="MJB72_001564"
     CDS             18805..20304
                     /gene="cobA"
                     /locus_tag="MJB72_001564"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBY8262683.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            20321..21286
                     /gene="hemB"
                     /locus_tag="MJB72_001565"
     CDS             20321..21286
                     /gene="hemB"
                     /locus_tag="MJB72_001565"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBY8262684.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            21439..22017
                     /locus_tag="MJB72_001566"
     CDS             21439..22017
                     /locus_tag="MJB72_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBY8262685.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT         9090 a         2121 c         4170 g         6726 t
ORIGIN      
        1 atatttgaaa ggtagatttt agtatatttt gaaaattttg ttttgaaaat ttgggattat
       61 ttaagaaatt ttgtattata aatagaagga aatagataaa ttgtatagaa taaaatatat
      121 taagataagt taataatgtt aaatgggaag tgagtttatg gaaaaaccaa atacaaaagt
      181 tgttaacttt caagagtata aagacagaaa aaaccaaaga gaagtaaata gagacgaact
      241 tttaaagctt ataaaaaaaa ttgtgacttc aaaataggga gttgttaaac tccctatttt
      301 aatgggtttc ataataatta agacattcta agtccatcta tataatcttt gaattgtgag
      361 aaatctgatt cactacttaa ttctatggtt tctgaacctt ccgtataaaa attcttaaga
      421 tagatagtat aattattatc aacacaatca tgataaatac atctatatcc atcttcatac
      481 aaacttttta acttagaaaa atcatccaca atatcatctc cttaaagttc ttttaaatat
      541 atttttacaa aaagcatcta attgtatgca aattgacaat gtaaaataaa ggaaatgaac
      601 ataaaatgta gaataatttt tatatgtatg taaaaataaa aagaaaaagt tgaaattttt
      661 agaaaataag agtatttttt atctaaaaag agagtttaaa aaataaaaaa atgttgggag
      721 gataaaaaat gagtttatta gaaagtatat caaaaaacat atattcccta gataactctt
      781 ctattgaaaa aacaaagcag agattggata ggcttataca tccaactgga agcttaggaa
      841 agatagaaga tatttgcatg caattagcag gaatatttgg caatgaaaat tttgacacaa
      901 gtaaaaaggt tataattgct tttgcgggag accatggtgt atatgaagaa ggcgtagcac
      961 ctgaccctca aaatataaca aaattacagt ttccaaattt ttcgaaaggt ctatgcggag
     1021 ttggagtaat aagtaaattt gtaggggcag atgttgtagc tgtagatgta ggaataaact
     1081 gtgatgaaaa actggatggg gtattagact acaagataag aaaagggaca tcaaatatgg
     1141 caaaaggtcc agctatgagt aaacaagaag caattagatg tttagagata ggtattgaaa
     1201 tagcagagca gtgtatagaa aaagattata aggttattgg tattggggag atgggtatag
     1261 caaatactac tccaagtaca gcaataatat ctgtaatttc agggtgtgac ccgttagaag
     1321 ttactggtat aggtgcaggt cttaagaaag aaagactaaa gcataaggct gaagtaatta
     1381 gaaaggctat tgaaattaat aatccaaatc caactgatgg agtagatatt ttatctaaag
     1441 taggagggtt tgaaataggc tcaatggcag gtgttatatt aggttgtagt gctaatagga
     1501 ttccagtagt aattgatggg tttatttctt atgcagcagc attaattgca tataagataa
     1561 atccaaaaac tagagaatat atgatagcat ctcatttatc agcagaatca ggaactaaaa
     1621 gagcattaga catattaaaa ttagaccctc tattaaatat ggatatgaga ttaggtgagg
     1681 gaagtggagc tgctttagca tttgatataa tagaagcttc aaactatact tataaaaata
     1741 tggcaacttt tgatgaaatt gacatgggaa gataaatata aaaatttaat atgtaggagt
     1801 gttttgatga gtaaaattat tctagtaaca ggaggagcta ggtcagggaa gagtaacttt
     1861 gcagaaaaac tatgttttga cagaaataat aatacagctt atatagcaac ctctattcca
     1921 tttgatgatg aaatgaaaga tagagtaaaa aaacatcaag agaatagacc tagaaactgg
     1981 aatacttatg aaatatatga agatatatat tccataatta aggagatata taaagaacat
     2041 gaaactgtta tactagattg tgtaacatta ttagtaaata atctaatgtt tgaacacaat
     2101 ctaaatatag aagaatctag tcaagaagaa ataaacaaat tagaacaata cataaaagaa
     2161 caagttgtca aacttattga agaaatagaa aaaacaaatc tatactttgt atttgtaacg
     2221 aatgagttag gtatgggaat tgttcctggg aataaactta gtagaattta tgctgatatt
     2281 gtaggtcgta taaatcaata tatagcttcg aaaagtaatg aagtttattt tgtagttagt
     2341 ggcatagcta tgaagataaa ggagtagtat gcaattatga aaagatttat tttaatacta
     2401 cagtttttga caaggatacc tataaaatta aatgtaggat ttgatgatga attttataag
     2461 tcaatagtat attttccact tgtaggtttt gtcataggta tattatccta tttaatagga
     2521 tggatttcta tgcttttatt tgaaccattt atagcttcta taataataac tctagctggt
     2581 gtcttaatta ctggaggact tcatatagat ggattaggag atacatttga tgcaatatat
     2641 agttataggg ataaagaaaa gatgcttgaa ataatgaaag attcaagatt aggtacaaat
     2701 tcacttctag caattatgtt tgtactttta ttaaaggttg gattcgttta cgatataata
     2761 agtaataatt cattgtgggt tataattttt atgccaatga ttgcaaggtt aggagtaatg
     2821 ttattgacat ataaaactgt gacaccaaga gaaaaaggaa tgggaaattt atttatagga
     2881 aaattaacta caagtatgtt aataactgct ataatataca cattattgat agttgctctt
     2941 attactaaat ttatattttt attacctaat atagtgttga ttaaagtatt aggttcgatt
     3001 atagtggttt ttgtatttat aatattattc aaaaaacata tttataaaaa aattgacgga
     3061 gtaacaggag atatattagg ctgtggaata gaactttcag agcttgtata tttaatttac
     3121 atatatttgt taatttttat gtttttttaa agtaaaaagt ggtgataata tgataagatt
     3181 aatattaata agacatgcac tgacaaatga taataaaaaa ggaagattat caggccatat
     3241 aaatagttgt attagtgaag agggcaaact acaaataaat aaaattacca gatatctgag
     3301 taatgaaaac atagataagg tttatacaac accatctaca cgtactaaag atacagtaga
     3361 aaaaatatca aaattgaaat tattagaaat agaagaaaaa gaagctttaa gagaaattag
     3421 ttttggagac tttgaaggaa gaacttttga agaaattaaa atcaaatatc caaatgagtt
     3481 tgaaaaaatg ataaaagaag gtaacaacta tagatatcca aatggagaaa gtttgataga
     3541 ctcttataaa agagtggcaa aagaaattga taatataatt ttagaaaata atagtaattt
     3601 agatacaaag acaatactta tatgttctca tgcaggaaca ataagaaata taataacaca
     3661 tttgatttct ggtagttata agtatcactg gaactttaaa attgataatg catctattac
     3721 agttttagaa attgatggtg gatttgctgt aatagataaa atgaatttta cagacttcat
     3781 ataatttaat caaaaccaaa cattacaatc tgtgtttgta atatatctgt aaaattagca
     3841 atgaaaatag aatattagat taaaattagg ttcttaatta gatgttaaaa gggaaaaagg
     3901 ttagatgcct ttgcagcccc cgctactgtg aaaccaacga aactataaat accactgtca
     3961 gtttgatggg aaggttatag aagtaggatg aagttaagcc aggatacctg cctaatttaa
     4021 tttaaaacaa gaggtcttcg gggtgaaaga tttttaatta atatatataa aagtcctatc
     4081 tttagaagta ttaggacttt ttttaatttg ttttttagta aattttaaca aggagggatg
     4141 gaaagtgggg aaaaaaataa tgctacaagg aacagcttca aatgtaggta aaagtgtctt
     4201 agtagcaggt ttgtgtagaa tatttaagca agatggttat actgttgctc cttttaagtc
     4261 acaaaatatg gctttaaatt cttttataac taaagaagga ttagaaatgg gaagagcaca
     4321 agtatttcaa gcagaggctg cgaaaataga gccaatagct gatatgaatc cagttttatt
     4381 aaaaccttct ggaaataata aaagtcaagt tattgtaaga ggtaaagttg taggagaaat
     4441 gccttcttct aagtatcatg attataaatt ggagttagta gatgtgttga aggaaacatt
     4501 taatagctta aattcaaggt atgatattgt agttatggaa ggggcaggaa gtaatgcaga
     4561 gataaactta aaagatagag atatctctaa tatgggtatg gcagaaatag ctgatgcacc
     4621 tgtaataata gtcggagata ttgatagagg aggcgtattt gcctcattag caggaacaat
     4681 gcttttatta aatgaagatg agaaaagaag agtaaaaggt gtaataatca ataaattcag
     4741 aggaaaaaaa gagcttttac aagatggtgt aaagatgcta gaagacatca taaaagtacc
     4801 tgttatagga gtagtacctt atacagatat aaaaatagaa gatgaagaca gtgtaactac
     4861 gagatttaag aaaaaaatga ataaaagtga tatacacata gaaataatta gaaccccaca
     4921 tatgtcaaat tttacagatt ttaatatatt tgagacacaa gaagatgtaa gtataagata
     4981 tgtagactat ggagaatctt taggaaatcc agatatatta ataatacctg gaactaaaag
     5041 tactatagac gatttaacat atataagaaa aagtggtctt gaatcgcaaa tcaaaaattt
     5101 acatagtcaa ggtaaattga tatttgggat atgtggtgga taccaaatgt taggtaaaaa
     5161 attaaaagac ccttatcatg ttgaaagtga aattgaagaa gttgatggaa taggtctttt
     5221 agatacagaa acaacgtttg agaaagaaaa gactacaaca caagtagaag ctactattgt
     5281 tgaatgtatc agtgaataca tgaataacct taagggaaaa aaagtaaaag gttatgaaat
     5341 tcatatggga attacaaagc gaggtaatga tgtaagaagt ttagatttaa taactaaaaa
     5401 acttgataaa gaagtgaatt atactgaggg aagtgtaaat aaagagggga atgtaattgg
     5461 gacttatctt catggaatat ttgatgaaat aagctttaca agagcaattt taaacaatat
     5521 aagaaagaaa aaaggtttag gagaattaga aagcaaagta aactctttta atgaatttaa
     5581 ggataaagag tatgataagt tggctgattt tttaagagaa catttagata tagagaaaat
     5641 atatgagatt atgaaataat gatgaccttt atttataatt taggtaggag gtgtattatg
     5701 aaaaaaattt taattgcggg tactaatagt ggagtaggta aaacaacaat atcattagga
     5761 ataatgcaag ctttggttaa gagaaatatg aaagtacagc catataaagt agggccagac
     5821 tatatagacc cttcatatca cacttttata actggaagac attctagaaa tttagattct
     5881 tatatgttag atgatgaaaa aataaagtat atagtaaata agtcctcaaa agatgcagat
     5941 atttcagtaa tagaaggtgt tatgggctta tatgatggtt ttggaataga tttagataat
     6001 tgtacaagtt cacatacttc aaaggtatta aagtcacctg ttatattagt tataaatggt
     6061 aaatctatgg cagcatctag tgctgctatg gtacttggat ataaggaact agataaagat
     6121 gtaaatataa agggtgttat agtaaataat gtaaagacta atagccatta tcaaataatt
     6181 aagagttcta tagaaaaata ttgtaatgtt gaagtattgg ggtattttcc tccaaataac
     6241 aaattctctt tggaatctcg acatctagga ttagttccta gtgtagaaat gaaatcacta
     6301 agggaaaaat tttatacctt agcagatgaa attgaacact atatagatat tgacagaata
     6361 attgaaatat cagaaagtga tgaatttgag agtgattttg attttcaaac tttgatagaa
     6421 aataataaaa taaaagaaca tgttaataat aaatcaatag ctatagcata tgataaagca
     6481 ttcaattttt attatagaga aaatatagag cttttagagg aacttggttt agaaataaac
     6541 tattttagcc ctcttgaaga tgcatcagtg ccaaatgcag attatatata tattggagga
     6601 ggttttccag aaatatttgg aaaagaatta gaagcaaatg agtcaatgag aacttccata
     6661 ttagaagcac ataacaataa tatacctatt tatgcagaat gtggaggact gatgtaccta
     6721 ggtgagaaac tattgaatca agaagaacaa gaatttaata tggttggaat atttagtgga
     6781 attagtaaga tgacatcttc tttaaagaga tttgggtact gtattggtgt agccaaggaa
     6841 aatactcttt tatctaaaaa aggggaatct attaaagggc atgagtttca tcattcaata
     6901 tttgaaagtg atgaaaaatg tgtatattca atgagaaaag aaagagatgg aaatatagta
     6961 gaagagtggg atggtggtta tagtaaagga aacactttgg ctacatatct tcatactcat
     7021 ttttataata acttaaattg tatagaaaat tttttgaaaa aaggaaatga ataaatgagt
     7081 attttatcta tttatatagg atatgtgaca gacttaattg taggagaccc atattctttt
     7141 cctcatccag tgagatttat tggtaaactt ataaatttta ctcaatgtat aataagaaag
     7201 gtctctaaaa atgataggca gttaaaattt gggggattta tattatggct tataaccgtt
     7261 ggaataacat atttaacaac ttatgctata gtgaagttat ttagttttaa tatattattg
     7321 agtattgtag tgaatagttt tataatttat acaacactag ccacaaaatg tttaaaagac
     7381 gaagcattaa aaatttataa tgtattaaaa actgaagata tagaaaaatc taggattcag
     7441 ctatcttata tagtaggtag ggatactact aatttaagtg aacctgaaat aataagagca
     7501 actgtagaaa ctgttgcaga aaatactgta gatggtataa ttgcgcctat gttttatgca
     7561 tttattggag gagcaccact tgctatggcc tataaagcta tcaatacatt ggactcaaca
     7621 gttggatata aaaataaaaa gtataaagat attggttttg catctgctaa gattgatgat
     7681 atagccaatt atataccagc tagaatatct gtcattctta tgacaattgg aagtttcttt
     7741 ttaaattata actataggaa ctgttttaaa atctctatta gagatagaaa gaatcataaa
     7801 agtccaaatt gtgctttttc agaaggtgca gtatctggtg ctctaggaat acaactagga
     7861 ggtaccaacg tttattttgg ggaaaaagta tataagccaa caatagggga taagcttagg
     7921 gagatagata aagaagatat aattaaaact aataaaatta tgtatgcatc ttcatttgta
     7981 tctatgttgg tatttacact aatatatgtg ttatgtagga tgattttaat taacttaatt
     8041 atataaatta ttgtttgtta acttaagggg gacatagatg aaggatttag ggcatggtgc
     8101 caatgtagat gaaatggcaa gattatatgg gaaaaaccca aaagaaataa ttgattttag
     8161 ttcaaacata aatccaaatg tattaccaaa tttagagaga tacattttaa aaggattaga
     8221 agagtgtagg aattatccag atataaatta tacaaacttg agagaaaata tatcaaaata
     8281 tatagatata aatccagact ttataatacc tggcaatggt gcaacagaag tgatttattt
     8341 gcttatgaag agtattaaaa aaaaattggc tataataaat ccgacttttt cagagtatag
     8401 aagaagtgct gagttaaata atttagatat tatagattta gaattagatt tagaaaataa
     8461 ctttaaactt aatatagata taataaaaga aaatataaaa agatttgata gtttatttat
     8521 atgtaatcct aataatccga gtggtaatgt ccaagattta aaggaacttg ttcatttact
     8581 agataaacat aataaggtac taataattga tgaaactttc atggaatttg ttgaagatga
     8641 aagtaaatat agtttggtta aatatataga atcaaataaa aatatattta taattaaagc
     8701 tgtgactaag ttttttggta tgcctggttt aaggctagga tatggtctta caagcaacac
     8761 tgaaattatg aacaaaattt atgaacacaa agagccatgg actatcaatt cttttgcaga
     8821 tatactatcc aattttattt ttgaagataa agagtatatt aaaaatagta aagaatatta
     8881 catagaagaa agaaaatata tgttacaaga gttgagaaac ataagaaata taaaggtcta
     8941 tgatacagat gctaatttta tcttgataag aatatataaa aaaacaacta aagaattgaa
     9001 aaaagatttg tttaagcaag gaaacatatt agttagagat gcgtctaatt ttataggatt
     9061 agatgatagt tttataagag tggctataaa atctcatgaa gataacaaaa ttcttataga
     9121 aaatatgaaa aatttattgg gtgattaaaa tgaaatctta tggaatatgc ccagcatctt
     9181 gtggagaatt tgtacaaggt ataattgatg atgaagaata tttatgttca tatgcaatag
     9241 atatgtattc taaagtgtat attgaagaaa aattagtaga tataaattta gggagatata
     9301 agtcaaggtt agcaattgaa aaggtatttg aaaaatttaa tttacctaaa aaatatacta
     9361 aaaatatttc tttaaatata aatagtaaaa tacctgttgg aaaaggaatg gcaagttcaa
     9421 ctgctgatat aggagctact ataaaggcaa cattatcatt gatagataaa gatttaagta
     9481 gcgaggagat ttctaaatta gcagcagaaa tagaaccaac agattctata tttatagata
     9541 aaaatagtat atttaatcct ctaaatggaa ctgttataaa atacctagga aatctgacca
     9601 atgcgaaagt tgtaatactt gaaccaaaca aagtactaga tactatgaag attagattga
     9661 ggcaagatta taataaatta aaagtagaga ataaagaagt aataaaaaaa tcatttgctc
     9721 ttttagaaga aggattgaaa aaaaataatt tatctctagt tggagaggct tgtactttga
     9781 gtagcttagc aaatgagaat atagaaaaaa aagagtgttt aaatgaaata ataaaaatat
     9841 caaaaaaata tggagcatat ggagtaaata ttgctcacag tggaactgta gtaggtattt
     9901 taattgataa gtctatgaat gataaaaaga tgatagatgc cttatgtgaa tctaacataa
     9961 attctgtcta taataaaatt tatacacaaa acataattaa tggaggaatt aagggggaaa
    10021 tagaatggaa tacataaaga atcctatgaa aatagaagaa aaaagttttg aattgataca
    10081 agaaataata gacgaaatta gaccagatta taaatttaag aataatatcg aagaaaaaat
    10141 aataaaaaga gctatacata ctactgctga ttttgattac ttagatatat taaaaatatc
    10201 agaagaagca gttgatagta taatagatgc attaaaaaat aatgcaagta tttatactga
    10261 tacaaatatg gctcttagtg gtataaataa aagaaagtta gaatcattag gatgtaagta
    10321 taaatgttta gtggcagatg atgaaactgt aaaattagct aaagaaaaag gaattactcg
    10381 ttctatggca gcagtagaaa ttgcagctaa agaagaagga agaaaaatat tcgttttagg
    10441 taatgctcca acagctttat ataaagttat ggagatgaaa tcagaaggta gattagattt
    10501 agatgcagtt ataggagttc ctgtaggttt tgtaggagca caagaatcaa aagatgaagt
    10561 tgaaaaaaca gatataccat atataatttc taaaggtaga aaaggtggca gtaatttagc
    10621 agcagcaata gtaaatgcta tattatatac tatgtaggta cctatatgga agaatatgta
    10681 tatatagatg gaaaaaaata tagaagagga tatactacag gttcatgtgc aacaggtgca
    10741 tctaaggctg ctgtatatat gttgattaca aaaaatagaa tcaatactat aaatatagat
    10801 actcctaagg ggataccatt actactaaaa gttgataata taaatatttc tgatacattt
    10861 gtggaatgtt ccataaaaaa agatggtgga gatgatatag atgcaactca taccatggat
    10921 atttatgcaa gagctgaaat tgtagctaaa aatgataaga acaaagggta tcttactcta
    10981 aaggatatag atagtttaag caccaatagt gagtgtaaaa gtgagctcta taagtttatt
    11041 agggtttatg gcggaactgg tattggtgtg gttacgaaaa aagggcttag tgtggatgtt
    11101 ggtaagcctg ctataaatcc gactccactt aaaatgataa atcatgaaat aagaaagctt
    11161 ataggagata attttgaatc tatacttgga aatgataaag ttttaaaaat aacaatcttt
    11221 gctccacaag gagaaactgt agcaaaaaaa acatttaatc caagattagg tatagttgga
    11281 ggaatatcta taataggtac aactgggatt gttgaaccta tgagtgatga gggttggaag
    11341 aagtctctat ctatagaact tcagatgaaa aaagaacaag gcttagataa aataatattg
    11401 gttccaggaa atcatggaga acagtttata agagaaaaat taaacttaga tattaagtat
    11461 gtagttaggg taagtaattt tataggctat atgattaaag aagctcaaag aataggatat
    11521 aagaaaatct taatggcagg tcatataggt aaatttataa aagtatctgc tggtattttt
    11581 aatactcata gtaaggtagc agatgctaga agtgagatat tagtggcaaa cttagcttta
    11641 atgggagcta gatacgaatt tttaaataaa ataaatcaat gcgtaactac tgaagaagct
    11701 gtagagctaa taaataatag tgaatataga gaagtatata atatattaag taataaatgt
    11761 agggagagag taaagcaata cttgaatgaa gattcagatg atattgatgt ggaagtaatc
    11821 atattttcaa tggataagtc tttattaggt aaatcggata atacagatga tttagtggag
    11881 gtctttatat gataaacata ataggtctag gacctggcaa cttagactat ataacaaaaa
    11941 aaggtgaaaa tttaatttcc acttctgatg tattaattgg tggtaaaaga aatttagagt
    12001 ctataaaaaa ttttgaagga gaaaaaatag ttctggattc taatttaaga gaaattatag
    12061 agtacataaa taacaataaa gaaaagcaaa tatcaataat agcatctggg gacccattaa
    12121 tatatgggat aggtagatat ctatctaaaa atattgataa taagatgtta aacatggttt
    12181 ctgggattag ttcattacag tacatatttt caaaaatata tgtagatatg aatgatgtat
    12241 atattacaag tagtcatggt aaagtaccag attttgatta catattatca cataaaaaag
    12301 tatgtatggt tacagattca aagataggac caaaacaaat aagtagagaa ataatagata
    12361 gaaatctaaa taaaattatt gttgtaggag aaaatctgtc atatgacaat gaaaaaataa
    12421 caatagcaaa accagaagag ataataagaa tagacaattt tgatatgaat gttgtggtga
    12481 ttttagatga ggaatagtga atttataaca ggtaaagttc caattactaa ggaagaggtt
    12541 agagcaatat caataagtaa gttaaattta gtaaatgcta aaaacctcat agacataggg
    12601 gctggtacag gaagtgtaag tgttgaagct gcatataatt atcctaattt aaaggttata
    12661 tctatagaga aaaatgatga tgctatagag cttatagaga aaaatataaa aaaattcaac
    12721 ttaaaaaata tagaagtgat aaaaggatgt gctccaatta atctagggtt aaatgttaaa
    12781 gttgagtcta tttttcttgg gggaacagga aataatctgg aagaaataat agaatggtct
    12841 aaaaaaaatc tcgttacagg aggaagactt gtagctaatt ttataataat agatacattt
    12901 aatcaaactt taaaactgct taaaaagcat ggatttaaag aaatagatgt atgtgttttg
    12961 aatgtttcta aactggagaa actaggaaaa ggtgaatatt ttaaaccact aaatcctata
    13021 tatataatat cttgtgaaaa aggggaaaat gatgatgaat aaagtacatt ttgtaggagc
    13081 aggacctgga gataaagagc tgataacatt aaaaggatac aaattattaa gtaatgcaga
    13141 tgtagtgata tatgcaggtt cacttgttaa tcctgaactt ttggaatact gtaaagaaga
    13201 ttgccaaata cataatagtg cacatatgga tttacaagag ataatagatg ttatgagaga
    13261 aggtatagaa aataataaat ctgttgtaag actgcaaaca ggtgattttt ctatatatgg
    13321 ttcaattaga gaacaagtgg aagatttaaa taaattgaat atagattatg actgtactcc
    13381 tggagtaagt tcttttttag gggcagcatc atctttaggg gttgaatata cagttccaga
    13441 aatttctcaa agtgtaatta taacaagaat ggaaggcaga actccggttc cagagaaaga
    13501 atcaatacaa tcatatgcta agcatcagac atctatggta atatttttat ctgttcaaga
    13561 aattgagaaa gttgtgtcta aattactgga aggtggatat ccaaaagata ctcctatagc
    13621 agttatatac aaagcaactt gggcagatga aaaaatagtt aaaggaactt taagtgatat
    13681 agctgtaaaa gttaaagaaa acaacataaa taagactgct ttaataatgg ttgggagatt
    13741 tttaggagaa gagtataata attcaaagtt atatgataag gatttcaaac atgaatatag
    13801 agggtaatat tgctataatc tgtattacag aaaatggaaa aaaattagct ctaaagataa
    13861 attctctagt aaataactgt gttatatatc aagtaaaaaa taaaaaaagt aatttagttg
    13921 ttgaagaaac atgtgtacat acagttcaaa agaagttaag tgattttgta ggagaaatat
    13981 ttagtaaatt tgattacata gtatttataa tggctacagg tatagttgta cgaagtatag
    14041 caccatacat aataagtaag tttagtgacc ctgcaatttt ggtaatggat gaaaaaggca
    14101 aaaacgtaat aagtttattg agtggtcata ttggaggggc aaacagcttg acacagtaca
    14161 taagtagtct tattgatgct aatccagtaa taacaacagc tactgatata aatcaaaaag
    14221 cttccttaga tatgatagca aaaaaactaa atgcacatat tgataatttt agagaaagtg
    14281 taaaagaagt aaattctatg ctagtgaata ataaatgtgt tggattatat gttgatgaag
    14341 gatatcaagt tgatactagg ggatttaaac ttatagaaag taaggatgag ttaagagata
    14401 gtttttcaaa cacatatata gaaaaattag ttgtagtaag caataaaata aattgtattg
    14461 aagatattga gaatttaaac ctagataata aatttgaaca ttgctatagt acccaaaaaa
    14521 taataaaggt aattccaaga gatatagtga taggaattgg ctgtagaaga aacacagata
    14581 gtgaactgat gtataattca cttgaagagt ttcttaataa acaaaatata gatattagag
    14641 caataaacac agttggaagt atagaactga aaaaagacga aaaagcaata attgaattat
    14701 caaaaatatt gaaggtgcca tttaaaacat tttcagtaga tgaaatatct aaggttgata
    14761 atctttttga taaatctgat tttgttaaaa aaaatgtggg tgtgtattca gtagcagaac
    14821 ctgtagcata tcttttgagc aaaggtaatt tgttagttga aaaacataaa tataaaggaa
    14881 taactttttc agtggggaga ataaaaatat gatatacgtt gtaggaattg gaccaggaag
    14941 taaagataca atgactttag aagcaataaa agcaatagaa gattctgagg ctattgttgg
    15001 atataagact tatataaaac taatagaaga atttatacaa ggtaaagaaa taatccaaaa
    15061 tggtatgagg caagaagttg atagatgtaa acaagcagtg gaggaagcaa aaaaaggaaa
    15121 aaaagtagca gttgtaagca gtggtgatgc tggaatatat gggatggcag gtcttatttt
    15181 agaattaata tctaaagaag atgaagatat aaaagtaaag gtaattccag gcgttactgc
    15241 aagtattggt gcagcagcta tacttggagc tcctattatg catgatttct gtcatataag
    15301 tttgagtgat ttaatgactc cgtgggaagt aattgaaaaa agattgagat tggctgctga
    15361 agccgatttt gtaatttgtt tgtataatcc aagaagtaag ggaagaagtg aacatctaag
    15421 caaagcattc aaaataatgg gtgagtttaa aggtggagat acacctgttg gagtggtaaa
    15481 agatgtaggt agagaaaaag aagaaaagtt tgtatgtact tttgaaaata tggactttga
    15541 aaaagtcgat atgactacaa tggttataat aggaaataag tctacctata ttaatggtga
    15601 attgatgata actccaagag gttatacagt atgattttaa ttttaggagg aacttcagat
    15661 agtttatcta tatgtgataa gataaatgaa ctgaaaaatc aaccatatat cctttcagtt
    15721 actacagatt atggaaggga acttgcacaa caacatgcag agaatgtaat tttaggtaag
    15781 cttggaaaag aagatatgct aaaatttata aaagataata atatagtaaa aataatagat
    15841 gccactcatc cttatgcagt tgaagtatcc aaaactgcta ttacttgtgc aaaactattg
    15901 aatatagatt atataaggtt tgagagaaaa tctttaattg aagaaataga ttatgataat
    15961 aaatttatag tagatacaat tgaagaagcc tgtgaaatag caaataaaat agggacaaat
    16021 atattcatag gaacaggaag taaaaatttg aacagattta tagaggaaat aagtgataaa
    16081 aatttagttg ttagagttct tccaacgagt gaagtcatat taagttgtga aaaattaggt
    16141 ttaagtgcag ataatatagt agctatgaaa ggtccattta gccagtatat aaatgaagaa
    16201 acatataaac attacaatat agatttagtc ataacaaaag aaagtggaat agctggtggg
    16261 ttcttagaga aggtaaatgc atgtaaaact ttcaatatac cagtaataat tataaaaaga
    16321 gaatcgatta actacgaaaa tgtatttaat gacataaata aaattaaaga catacttgtg
    16381 taatttttag gaggagaata tatgaagaaa gccttattag tagtgagttt tggtacaagc
    16441 tatcatgaaa cgagagaaaa gacaatagat atatgtgaag ataaaataaa aaattcgtta
    16501 aaaagccatg atttttttag agcttataca tcaaatatga taataaataa aataaaaaaa
    16561 agggatggaa tagaaataga taatccaata caggctttag ataaaatata tgaacaagga
    16621 tatgatgaag taataatcca aacacttcat attatatgtg gcgaagaatt taacaaatta
    16681 aaagaacaag tagatggata ttcttctaaa ttcaaaaaga ttgtattagg aagacctttg
    16741 cttacacata ttgaagatta tcaggaagct gtagaggcaa ttaagcatca aattcctcat
    16801 atggaaagta atgaagcagt agtatttatg ggacatggaa cacttcatga atcacattca
    16861 gcgtatccag cattggaata catgcttagg gataatggaa taaatgctta tgttggtaca
    16921 gttgaagggt atccagaaat agaacatgta ataagaagat taaaagaagg aaatataaaa
    16981 actgtaaatt tgatgccatt tatgttagtt gcaggagacc atgctataaa tgatatggca
    17041 ggagatgaag aagattcttg gaaaacaata ttagaagaaa atggatttaa cgtaagagtt
    17101 catttaaaag gactaggaga aaattcttat atacaagata aatttgttcg tcatgctgta
    17161 aaatgtgcag agaatgcgaa gttctatggt ataggagcag ggccaggaga tggttcttta
    17221 cttacgataa aagcggttaa tactttaaag aaactggata ttctatacac tccagaatca
    17281 aaaaagggtg gagatagtct agctttatca atagttagtg aatatttacc tgaatcagtg
    17341 gaaataaagt ctaggcattt cccaatgagt tttgatggga atgaaaaaag tttagcttgg
    17401 gacaatgtgg cacaagaaat tatatcagat gttaatgatg gtaaaaatgt aggatttgtt
    17461 acattaggag accctatgat ttatagtaca tatgtatata taatgaaaag aatattagga
    17521 gaggtagaag tcgaaactat accaggaatt tcatcatttt ccaatatagc atctaatcag
    17581 aattttccat tagtcatgga taaagaagct ttagtggtaa ttccttgtac tatggaagat
    17641 gataagatag agtatgcttt gcaaaattat aattcaatag tattgatgaa agtatataaa
    17701 aattttaaag aaataataaa aaaattagaa aagtatgatt taatagaaca tgctatttta
    17761 gtaagtaatt cttcccaaga aagcgaaaat gtgtttacag atttaaaaga agcgcacctt
    17821 gaagataaga tatcttattt ttctactata ctagtaaata aagataataa aataaaatag
    17881 gtgaaagcaa tatttaaagg agacttaata tgaatatagt ggttggaact aggggtagta
    17941 atctagcgct tatacaaaca gaatgggtaa taaatgaatt aaaaaagaag tatccagaaa
    18001 ttagttttga gattaaaatc ataaagacaa aaggagattt aattcaaaat gtttcactgg
    18061 ataaaatagg tgataaagga ttgtttgtca aagaaataga gcaacaatta ttagatggaa
    18121 aaatagacat agcagtacat agtatgaaag atatgccttc ttatttagca gatggactaa
    18181 aatttgctca tacgcctaag agagaagacc caagagatgt tctaatactt agagaaggat
    18241 ataaaaattt agatgatttg cctcatggag cagtaatagg aacaggaagt aaaagaagga
    18301 agtttcaatt attgaaacaa agacctgatt taaatatagt acaagttaga ggtaatgtgg
    18361 aaactaggat acgaaaaata aaagatgaaa acatgcatgg tattgtgttg gcagcatcag
    18421 gaataattag agctaattta caagataaaa taagttctta tttacctgtt gatgttgtta
    18481 ttccagcacc tgcccaagga gctttagcta tagaaattag aagcaatgat agtacgatag
    18541 agggaatagt aaattcactg aaggatgaga atacagaaat ccaaatattg gctgaaagag
    18601 gttttttaga tggtgtaaat ggaagttgtc atattccaat agcagcttat tgtgaaataa
    18661 tccaagataa aatacatctt actggcttat atggagattc agaaggaaaa aaagtagtaa
    18721 tcaagtctat agatggagat atttcttctc ctagagagtt aggtttaaag ttagctaaac
    18781 ttgtattgaa ggagtatgag aattatgaag ggtaaggttt acttagtagg ggcaggacca
    18841 ggtgattata aactcatgac tttaaaaggt ttagagtgta taagaaaatc ggatgtaata
    18901 gtatatgata gattagcaaa tagtagttat ttaagagaag ctaagcctga ttgtgaactt
    18961 atatatgtag gcaaggcatc aagtaatcat attttgccac aagaagatat aaatagaatt
    19021 atagcagaaa aagctaaaga aggaaaaatt gtaactcgtc ttaaaggagg agacccttat
    19081 gtatttggca gagggggaga agaagcagag actttagtag atgaaggtat agaatttgaa
    19141 gtagttccag gtataacttc tgctatagga ggtttatgtt atgcgggtat tcctataaca
    19201 catagagacc atgcttcgtc atttcatgta ataacaggtc atttgaaggg tgatgatagt
    19261 ggagaattaa actggaatgc tcttgcaaat aacaaaggta ctcttgtgtt tttgatggga
    19321 atttcaaacc tagagaaaat aagtgaaaac ttaatgaaag aaggaaaaga taaggataca
    19381 cctgttgctc ttataagttg ggcaactaga tataatcaaa gagttgttac atctacactt
    19441 gaaaatgttt atgaaactgc tattaaagaa gaagtaaaac cgccaactct tatagtagtt
    19501 gggagtgttg taggacttag agaaaagttg aacttctttg aaagtaagcc tttatttgga
    19561 aaaagtattg ctgtaactag gtctagaaat caaaatagtg tattggtaga aaaaataatg
    19621 gatttaggtg gaaatcctat agaaatacct actattaagg tagaaaaaat acaaaataat
    19681 attaacttag aaaatgaaat aaaaaatata aataagtata attatctaat actaactagt
    19741 aaaaatgccg tagaaatctt ctttgaaaag atatatgaaa tgaattttga tttaaggata
    19801 ttatctaatt taaaggtatg tgctataggt tctgctacat caaatgagtt gaaaaaaaga
    19861 ggaattatag cggatatagt accaaaaaaa tttgtagcag agtcactgta tgaagagtta
    19921 gcgcctattt taaatgatga cgataatata cttataccta gagcagaaaa tgctagagat
    19981 tatctagttg acaaacttaa ggctatttgt gaagtaacag aagtacatat atatagaaca
    20041 gtaatagatg aggagaaaaa aggtgagata ttagatattt taaattctaa tgacatagat
    20101 tatataacat ttactagttc ctcaacagta aataatcttg ttgaaattat aggaaaagaa
    20161 aatattaaaa tattagaaaa aataaaaaca ctatctatag gtcctataac tacagagaca
    20221 ataaatagtt tgggaataaa attgtacaaa gaatctgcta catctactat agattcgtta
    20281 gtagatttaa ttataaaaaa ttaattttgg aggcttgaat atgttaagaa gaccaagaag
    20341 actaagagca agtaaagcaa tcagaaattt ggtaagagaa actaaggtga atgtagaaga
    20401 cttaatatat ccactgtttg tagtagaggg ggaaaatata aaagaagaaa ttaaatcttt
    20461 accagatgtc tatcatttat caatagatat gctagaagac gaaataaaag aaataagtga
    20521 gttgggaatt gaacatataa tgttatttgg tgttccagat gaaaaagatg agtgtgcttc
    20581 acaagctttt aatgataatg gaatagttca aaaggcaatt agaaaggtaa aagaaataaa
    20641 ttcagatata aatgtaataa cagatgtctg tatgtgccaa tatacaagtc atggacattg
    20701 tgggatttta actgatactg gttatgttga taatgataaa tcattggagt atttggctaa
    20761 aatatctgta agccatgcta aagcaggagc tgatatggtt gctccatctg atatgatgga
    20821 tggaagaata caagcaataa gagaagcttt agatgaaaat ggattagaaa atgttggaat
    20881 catggcttat agtgttaagt atgcttcaaa ttattatgga ccttttagag aggcagctca
    20941 ttcagcacca tcttttggag atagaaaaac atatcaaatg gacccagcaa atagcaatga
    21001 agctataata gagactgaac ttgatatatt ggaaggtgca gatatactta tggttaaacc
    21061 tgcattatca tatttagata ttataagaag agttaaagat aattttgact tacctctagc
    21121 agcttataat gtaagtggag aatactctat gcttaagttt gctgtgaagg aaggtttatt
    21181 aaatgaagat gtaattcttg aatcaattac atctataaaa agagcaggag cagatataat
    21241 aataacttat tttgctaaag acattgctaa actgttgaaa aaataaattc tagtagtata
    21301 aagaatatca aaaagtatag gaattgtaat taactattat acagtagttt tgtttagaca
    21361 tttattgaag aataatagaa actaatttgc aatttctata ctttatttat gtaactaaat
    21421 aaaatttgga ggtagaagat gctatatcct attaatttaa aattagatga gttagatgta
    21481 gttataattg gtggtggaga agttgcatat aggaaatgta aaaatttctt agagtttaac
    21541 aaaaatgtta ctatagtttc taaacaaatt ttaaataaat tttatgattt aaaaggaaat
    21601 ataaaaataa taaaagatga ttataaagaa atatatataa aagatgcttc tgtaataatt
    21661 gctgccacaa ataatagaga gttaaatatg gaaataggat tatattgtag agcaaaaaat
    21721 aagctagtga atgtagttga taatatagaa atatcgaact ttactgttcc atcatatata
    21781 aaaagaggag atttacttat aagtgtgtct actgggggca agagtccttc attatcatca
    21841 aagataaaaa aagaaattga agagagatat actgaagatt atgaggagta tttaaatgta
    21901 ttaggagata taagaaaaaa ggttataaaa aaatacgaag ataaaagtaa aagaaaagat
    21961 gtgcttaata tgttggtaac acttgaccta gaagaactta agaagtttga tatataaaaa
    22021 ataaaataaa aaatgttaaa aaggtgttga cggattattt ttaagatgat atagtattac
    22081 ttgtccttaa gaaaagggca aacataa
//
LOCUS       DAGSHT010000046        21889 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGSHT010000046 DAGSHT010000000
VERSION     DAGSHT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.047"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            88..1686
                     /locus_tag="MJB72_001567"
     CDS             88..1686
                     /locus_tag="MJB72_001567"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBY8262686.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1921..4083)
                     /locus_tag="MJB72_001568"
     CDS             complement(1921..4083)
                     /locus_tag="MJB72_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HBY8262687.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGPTGATGVTGEIGPTGATGPTGNT
                     GVTGPTGPTGVTGEIGPTGATGPTGVTGSIGPTGATGPTGVTGSIGPTGVTGPTGPTG
                     VTGEIGPTGVTGPTGEIGPTGATGSTGVTGSIGPTGPTGATGPTGNTGVTGEIGPTGV
                     TGPTGVTGEIGPTGATGPTGPTGVTGEIGPTGATGPTGNTGVTGEIGPTGATGPTGNT
                     GVTGEIGPTGATGPTGNTGATGSIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGPTG
                     PTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVDANGTFTVQENG
                     VYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVLSGYFAGFLFGG
                     TTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
     gene            complement(4761..5285)
                     /locus_tag="MJB72_001569"
     CDS             complement(4761..5285)
                     /locus_tag="MJB72_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY8262688.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGMDYSDVEKN"
     gene            complement(5350..6102)
                     /locus_tag="MJB72_001570"
     CDS             complement(5350..6102)
                     /locus_tag="MJB72_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY8262689.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(6133..7431)
                     /locus_tag="MJB72_001571"
     CDS             complement(6133..7431)
                     /locus_tag="MJB72_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBY8262690.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(7448..9712)
                     /locus_tag="MJB72_001572"
     CDS             complement(7448..9712)
                     /locus_tag="MJB72_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBY8262691.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(9967..10785)
                     /locus_tag="MJB72_001573"
     CDS             complement(9967..10785)
                     /locus_tag="MJB72_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY8262692.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(10778..11476)
                     /gene="cbiQ"
                     /locus_tag="MJB72_001574"
     CDS             complement(10778..11476)
                     /gene="cbiQ"
                     /locus_tag="MJB72_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBY8262693.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(11479..11763)
                     /locus_tag="MJB72_001575"
     CDS             complement(11479..11763)
                     /locus_tag="MJB72_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8262694.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(11753..12505)
                     /locus_tag="MJB72_001576"
     CDS             complement(11753..12505)
                     /locus_tag="MJB72_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBY8262695.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(12687..12879)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(13011..14783)
                     /locus_tag="MJB72_001577"
     CDS             complement(13011..14783)
                     /locus_tag="MJB72_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBY8262696.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSIYSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(14797..15726)
                     /locus_tag="MJB72_001578"
     CDS             complement(14797..15726)
                     /locus_tag="MJB72_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8262697.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            15954..16142
                     /locus_tag="MJB72_001579"
     CDS             15954..16142
                     /locus_tag="MJB72_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8262698.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            16135..16836
                     /locus_tag="MJB72_001580"
     CDS             16135..16836
                     /locus_tag="MJB72_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8262699.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            16838..17761
                     /locus_tag="MJB72_001581"
     CDS             16838..17761
                     /locus_tag="MJB72_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8262700.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            17918..18841
                     /locus_tag="MJB72_001582"
     CDS             17918..18841
                     /locus_tag="MJB72_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262701.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            18834..19571
                     /locus_tag="MJB72_001583"
     CDS             18834..19571
                     /locus_tag="MJB72_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262702.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            19582..20355
                     /locus_tag="MJB72_001584"
     CDS             19582..20355
                     /locus_tag="MJB72_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262703.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            20371..20523
                     /locus_tag="MJB72_001585"
     CDS             20371..20523
                     /locus_tag="MJB72_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262704.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(20896..21504)
                     /locus_tag="MJB72_001586"
     CDS             complement(20896..21504)
                     /locus_tag="MJB72_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBY8262705.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(21682..21798)
                     /gene="rrf"
                     /locus_tag="MJB72_001587"
     rRNA            complement(21682..21798)
                     /gene="rrf"
                     /locus_tag="MJB72_001587"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6934 a         3824 c         2882 g         8249 t
ORIGIN      
        1 tttcttttta caacaaatac tcactacctt ttgatataat atttttatca attattatta
       61 tattcaagtt ttgaaaggag tattaaaatg agtttattag ttttcggaca taaaaatcca
      121 gatacagatt caatatgttc tgcaatttca ttagcacatt taaagaacaa actaggaata
      181 aaagcaacag cttatgcttt aggagatatc agaaaagaag ctaaatatgc cttggattac
      241 tttaaggtag atgcacctga aattttagat aatgttagaa tacaagttag agatttaaat
      301 tatgataaag tagtaccttt aacaccaact tcatctatat tagaggctta taaccttatg
      361 gatgaaaaaa atgttaaaac actacctgta acttatgatg atggaacttt tgctggtatc
      421 attactatga aagaaatagc taaaaatcta cttcatcaac actttacaac tatacacaca
      481 tcactatcaa acatttgtaa aaaccttaat ggtactattc ttgtggactg tggtgaagat
      541 ataaaaggta gagtagtcac tttatcattt ggaatggaga ctctaataga gactttaaaa
      601 gaaggagaca tagctattgt tggagataga tatgatagta ttgaatatgc tattgataca
      661 aaagttaaac ttttaatact tactaatcat acagagattt caaaagattt gctagctctt
      721 gctaagataa atggagtgtc tgttgtttct gttgaaagtg acaattataa aacttctaat
      781 gtaataaatc aatgtagttt cttagactct atagaagcta cagaaaattt aattacattc
      841 aaagaagatg attatatgga tgaaataaaa gagattatgc tagaaacaaa cttcagatct
      901 tatccaatat tagatgatga taataagttt ttaggtttag tttcaaaagg tcatctatta
      961 aacccatcta aaaagaatgt tgttttagtt gaccataacg aatatgctca aagtgctgat
     1021 ggaattgaac aagcaaacat agttgaaata attgaccatc ataaacttgg tggtatttct
     1081 actgatgttc ctatgtcatt tagagttatg cctgttggat gcaatagtac tataatttat
     1141 caaatgtaca aagaaaataa tgttgagata ccttatgaaa tagctgggct tcttttatct
     1201 gctatattat cagatacatt attatttaag tctccaacaa caactgatat ggataagaaa
     1261 gcttgtgaag aattaagtaa aatagcaaaa gtagacatgg aaaaatacgc tatggacatg
     1321 tttaaatgcg gtacttcttt agatgaatat actatagaag aaatagtaaa tatggatttt
     1381 aaagaattta atatgagtgg gcatagagtt ggtattggac aagtatttac cttagacata
     1441 gactctatat ttgctaaaaa agatgagttt ttatcttata taaattcgac tgattatgat
     1501 aaattaattc tttcagttac agatataatc aaagaaggtt cttatttaat ctataaagca
     1561 gaagatagct taattgctaa cgcatttggt gttgaaggtg ttcaaggcac atttgcacca
     1621 ggtgttgtat caagaaaaaa acaattagtc cctaatttaa ctactgcaat caaaaatttt
     1681 aaataatata aaatttttaa ataacgaaaa ctgtctcaaa tctatatttg agacagtttt
     1741 ttattatata tatattgata ataaagttaa ataaaaatcc ataaatttaa aatttttatg
     1801 aatatatttt attattattg ttattttatt cattctaaaa taggtaaaac tcaatatata
     1861 aaaatacttt tgtcatttaa ataaagccta aatagttttt aagattattt tagacacgtt
     1921 ttaagtcata acagtatcag ctattctaaa tatcgtcaaa gtagccgcag ttcctgctga
     1981 ttgcccattt cgtatcccta ctgttgtaga tgaaaagtta tttattgtaa aagtagtccc
     2041 accaaataaa aaaccagcaa aatatccaga aagtactcct ccgcctcctg gctcagtagt
     2101 aagccctgcc aaatcttttc ctcctaaaat tgggaatagg atagcgaaag aatattgatt
     2161 tatacttgat gaacctggtt gtaatgctac tgaaactgat atataataca caccattttc
     2221 ttgtactgta aacgtaccat ttgcatctac tgttatactt gaactatttc ctattattgt
     2281 ttgccaacct gttatagcat ctccattatt tagtgtttgt ggagatggtg catttacgag
     2341 gaagttagca actggctgag aggaattgcc tgttgctcct gttggacctg ttgggcctgt
     2401 tactcctgtc gctcctgttg gacctgttgc tcccgttggt cctatacttc ctgtcactcc
     2461 tgttggacct gttactcccg ttggccctat acttcctgtc gctcctgtat ttcctgttgg
     2521 tcctgttgct cccgttggtc ctatttctcc tgtcactcct gtatttcctg tcggacccgt
     2581 tgctcccgtt ggtcctattt ctcctgttac tcctgtattt cctgttggac ctgttgctcc
     2641 cgttggtcct atttctcctg ttactcctgt tgggcctgtt ggacctgttg ctcccgttgg
     2701 tcctatttct cctgtcactc ctgttgggcc tgttactccc gttggtccta tttctcctgt
     2761 cactcctgta tttcctgtcg gtcctgttgc tcccgttggt cccgttggtc ctatactccc
     2821 tgtcactcct gttgaacctg ttgctcccgt tggtcctatt tctcctgttg ggcctgttac
     2881 tcctgttggt cctatttctc ctgtcactcc tgttgggcct gttgggcctg ttactcccgt
     2941 tggtcctata cttcctgtca ctcctgttgg acctgttgct cccgttggtc ctatacttcc
     3001 tgtcactcct gttggacctg ttgctcccgt tggtcctatt tctcctgtca ctcctgttgg
     3061 gcctgttgga cctgttactc ctgtatttcc tgttggacct gttgctcccg ttggtcctat
     3121 ttctcctgtc actcctgtcg ctcctgttgg acctgttggt cctatacttc ctgtcactcc
     3181 tgttgggcct gttactcccg ttggtcctat ttctcctgtc actcctgttg ggcctgttgc
     3241 tcccgttgct cccgttggtc ctatacttcc tgtcactccc gtatttcctg tcggacctgt
     3301 tactcctgtt ggtcctatac ttcctgtcgc tcctgtactt ccggttgggc ctgttactcc
     3361 cgttggccct atacttcctg ttgctcctgt atttccggtt gggcctgttg gtcctatgct
     3421 tcctgtcgct cctgtacttc cggttgggcc tgttactccc gttggtcctg tacttcctgt
     3481 cgctcctctt ggtcccattg gacctgtaat tctaattgga agacactcac atttaggata
     3541 acaattatct ataattctat cttcaaatgt tacaagtata tacggtttgt tacaacctct
     3601 actagatgta aattcaagaa tctgataagg gtaatttaaa gataaagcta acccataatt
     3661 aggatattta ttcattgccc atgcttttat agttcccgta atatttaaac aaatatagtt
     3721 ggatgtatca cttatcccaa ttggtaaata tcccctgata ttttccatag acactctatc
     3781 attccatgtt atagttcgta aattataata ttcacttatt cttttaattt caatatctac
     3841 tttttcttta aaaatatgat tcttatttga atcaatatga ataaataact ctgcttttaa
     3901 tatacttttg tcttttaata tatctggtat tctcacttta aaaaaagtaa tcaacttttc
     3961 ttttacaaaa ctatttgaac atttacttcc tattaagcaa taatctaaat tactaaagtt
     4021 tttatctgga tattttttag atataaaagt atcatcattt aaataaagta taatatttct
     4081 catttataat ccctccattt tgggtctata tcatactatg tttaaaatac atgcttagtt
     4141 acaatacaat taaaatttat agtattaaat ttttatataa taatgctgat ttctacaaaa
     4201 aaagaacatg tcattagaaa tttctaatag catgttcttt ttcattatta tatattacac
     4261 tttaaattat tccttgactg gcttagctgg tgtaaactaa accagtcttt ctaaaatatt
     4321 aagcactttt ttcttattat gttttttagg atttattgtc tatattattg taaaccaaag
     4381 ttataaaatt gtaaatagaa tatattacca taacaatatt tttttataag taaaaattat
     4441 aaaaaacttt atttttgtta aatagaataa ataaaataag tttaaaaaat actgaaataa
     4501 gtgttttcca catattttat atatttattt aatacattat tatttaaaaa taaatatata
     4561 aacaatcata ataaaatttt tttaataaaa ctttattttt tagttaaaat catacaatat
     4621 atagatttcc acaatacaaa taaattaaaa agtgaaatgt atttgtctaa aaacataata
     4681 acatttcact ttatcctcat ttttaagtaa tatatatttt taatttttat ttataactaa
     4741 atctagtttt tatataaatt ttagtttttt tctacatctg agtaatccat tccaaaagtt
     4801 tctacagtaa cagatttcat aacttgtgga gtttgtggct tatcagctga atctgttcta
     4861 actgatgcta tttcatcaac tgtatccata ccttctatca ctctaccaaa acctgcatac
     4921 tgaccatcta agtgtggtga gtttttatgc attatgaaaa attgagaacc agctgaatta
     4981 ggtgccatag ttctagccat tgatatgact cctctttcat gttttaaatt gttagtaaat
     5041 ccattaccac taaattcacc ttttattgag tgacctggac cacccattcc tgtaccattt
     5101 gggcatcctc cttgtatcat aaatcctggt attactctat gaaatattat tccattgtaa
     5161 tatccttcat ttactaatga tacaaagttt ttaactgtat ttggagctat atttggatat
     5221 aactcaattt tgatttcttt tccattttcc atttctatag ttactatagg atttttattt
     5281 tccataattt attaccttcc ttttcattag ttaaattaca taattacatt tattatatta
     5341 tactctaaat taaagtctat caagtatatt ttttcctatt tcatatcctt cttgataaat
     5401 agttttacaa aactcatcat caaagttaag cgtgtcttta ggagcaaatt gagtattagg
     5461 tctaactact attattttat cttcatcata tactttttta atatcttctg gcaactcata
     5521 attatatttt gtggatatta aaataatttt atccacatta tccccaatta atggttcaat
     5581 taatgcactc ctaattaaac ctccatctaa tttgaaacca tcataaattc cttcttgcac
     5641 atctttcaca aattgttctt taaatccaag agttgcgttt ggatttgatg gtaacaaccc
     5701 actgtatgaa atgctgataa tggcttcatt acgaggcatt tttgatacgt caacaatctt
     5761 ttctgatact actttattat caacttcaac ataattaaca taaaagttca tttcttctat
     5821 attactatca tctagttccc ttaggttgtc tatagcagga gaattatcca cagtattatt
     5881 tactattttt acatttccat tctggatatc ttttatattt gtccacattt tctccaagtt
     5941 atcaacattc cctgtgtaca aataatatcc atttattgct cctattgatg tccctgaaat
     6001 cgaataaaat ttatttattc ctccatcata caaggcttta actactcctg cttgataagc
     6061 cccttttgct cctccacctt ctaaacataa tcctaatttc atagtctcat ctccaaattg
     6121 tttatcttat atttatattt caaaaaactt actaaatgtt ttatatatac tatagtggtc
     6181 ctcaacattt cctaattctc ttatagaatg cattgctaaa attggacttc ccacatccac
     6241 agatggtata tctatatgag tagaagatat tggacctata gtacttcctc ctctttcatc
     6301 tgacctatta acaaattctt gatactcaac attagcttct ctacatatat ttttataaat
     6361 agctatagaa tatccatcac tagtatatgc ctgactagca tttatcttta taaccggacc
     6421 ttttcccatt attggtttat tagttgggtc atgcttttct ggaatgtttg gatgaatagc
     6481 atgagctaaa tctgatgata tcataaaaga tgaataaata gactcaaaga atccctctct
     6541 atccttaccc attgatatac aaattctctc caatatattt aacaacatgt ttgaatctgc
     6601 accttgttta gtagagctac caacttcttc attgtcaaat acagccatta catttacacc
     6661 atgttgacct ttagtattaa caagagcatt caaactagca tgtaccattg caagattatc
     6721 ttgtctccca atagatataa attcatcatt agcccctatt aaacttcctt tttcaaattc
     6781 atataaaaat aaatcaaaat ctataatatc ttccatattt acatctaagt tagatgctat
     6841 ttgctttata agaaaattat ctttttctaa tgtatcattt aacatcccaa ctaaaggtaa
     6901 catatctttt tgcttattat atgaatgacc atcatttata cttctattta aatgtattgc
     6961 tagatttggt attatacaaa ttggcttttt aaagtctata agcctttcat ttggttttaa
     7021 tatactatca ccttttaaaa ctactctacc agctatccca agaggtctat ccaaccatgt
     7081 acttaatatt gctcctccat agcattctgt atttaacttc aaataagatt tttcagcttc
     7141 tatttctgca tttggtttaa tactaaatgt aggagaatct gaatgagagc ctattattct
     7201 aaatccatct ctttctatac cttctgaatt tactacaaaa gctactatag ctgataaatt
     7261 cttagtaaca taatactttc caccaactct tagattccat ctttgattca aaactaattc
     7321 ttcaaatcca tttttatcta agatttcctt agtactctcg actgcatgaa aagacgacgg
     7381 actatcatat atgaagtcta ttaagttttt agcaaagttt atactatcca ttcctcattt
     7441 acctccatta taaaatattt agtgttgtat attctttatt tattttttta attccctgtt
     7501 ggtcaaaagc tatagtaaca tctgtaccaa ttatagaaac cactgtccca accccaaatt
     7561 taggatgatg taccttagaa ccaagtttta tatcatctgg attagtttct ttattagaat
     7621 cttttattgt agcattaact ttatctgcaa ctttagattt gcttatagta ctcatatact
     7681 ttttagtata tttatctaac atattataac ttgctttaga atagcttagt tcttttttta
     7741 ctttatacaa tctttctata cattcttctg gcagttcttc cataaatctt gaagctattg
     7801 caacatttgt tttaccatat aatgttctct tctctgtcaa tgacatatat agctcttctt
     7861 tagctctagt tataccaaca taacaaagtc ttctctcttc ttctatttgt gaatcactca
     7921 tagattttat agctcttgat ataggaaata acccttcttc catacctgtt aaaaacacaa
     7981 cagggaactc taaacctttg gatgtatgaa ttgtcataag cgaaacccta tcattgtctt
     8041 cttcatcatt tgattcagat gtaagtgcta tacttgttag gaatgtctct aagtctttat
     8101 tttcataagt atcatcatta ctactttcaa actccaaagc tatagaaata aattctctca
     8161 aattatcaat tctgtcttga gcttcttcac ctttgcctgt taaatctttt tcatttttat
     8221 ttcttatctc tacaagctcg tccatatatc ctgtagtatc caagactttt tctataagtt
     8281 tgctaacagg ataaacttct tttatagttc taagagtccc tataatatca acaaatccac
     8341 ttatacttgc ccttgcctta gtagatatat ctgaatttgt ttctatatca attaatactg
     8401 agtatatact ttcctgtttt aagttagctc tatcttcaat tttttctata gttctaagcc
     8461 ctatacctct tctaggaaca tttataatcc tttttataga tatatcatct tgtggatttt
     8521 gaataactct taaatacgcc aacaaatcct ttatttcttt tctctcatag aattttgtac
     8581 caccatatat attgtatgga atttgacttc tatttaaagc gtcctctaca ggtcttgcct
     8641 gtgcatttgc tctataaagt actgcaaaat ccttataagg tctattttct tttctagcta
     8701 tttgtgcaat tgaatcagca atgaaatcag cctcttctat ttcgctacca gttaattgta
     8761 tctttataag ttctccttct tttttttcac tccaaagctt ttttcttttt ctttctatat
     8821 tattagatat gactttatta gctgcatcta atatcacttg tgtagaccta taattttgtt
     8881 ctaactttac aacatgtaca ttgtcataat ctttctcaaa ttcaagtatg tttcttatat
     8941 ctgcacctct ccatccataa atactttggt catcatctcc aacaacacaa atattttgat
     9001 gttctcttgc taataatttt atcaattcat attgagcttt acttgtatct tggtactcat
     9061 ctaccattat gtatctaaat ctacttctgt aatatgctaa cacttcatca ttagccttga
     9121 aaagttctac agttttaagt attaaatcat caaagtcaag tgcactattt ctttttaaac
     9181 ggtcttgata tagcgcataa atatctgcaa tcttaaccat tctattatct gccatattga
     9241 tatctttaaa ctgctttgga gtataaagtt tgtcttttgc tcctgaaatt gcacttataa
     9301 ctgcttttgg ttcaaacact ttatcactta aatttaattc ttttagacag tctttaacca
     9361 atgtaacttg gtctgctgaa tcataaatta caaaacttct attgtaacca atcttattta
     9421 tatcttttct tagtatacga acacagcaag aatggaatgt acttatccac atatccttag
     9481 tttcaggacc tatattttgt tcaactctct ctctcatttc atttgcagct ttgtttgtaa
     9541 atgttatagc caatatattt ggtgcttgta cacctttatc ttctataaga tatgctattc
     9601 ttgttgttaa taccttagtt ttacctgaac cagcaccagc cagtataagt actggacctt
     9661 cagtcttttc tactgcttct ctttgagcag gatttaatgt atctaaattc atatttctcc
     9721 tcctgttaac agccataaat atattttaat ctaaaatatg aattacttca aattataatt
     9781 atgtattata ttaatagatt atttccaata tattacaatt aaataacatt tttccatata
     9841 atttatttca ttgtttaata aaaaaaataa ccttatgatt tggggaatca taaggcacct
     9901 ttaaggggtc taggttaagg gttattatat ataaatctat ttttttaaaa taggtttagg
     9961 gcttaattat ttatttgtat taagttctct ttccttccaa tacttataca aatcttcttc
    10021 tttcttaaat aagggtaaat tcatatttct atgtactttt actaaccatg gggattctag
    10081 accagcttgt ataatattat tttcatttat aaacacattt tctgcttttc cttcatcaat
    10141 caaaatcccc ttatccaata catatatgta atcacatatt tcatacatta aattcatatc
    10201 atgactggat attactattt ttatattatt tttatttaat ccctttatta tatctacaat
    10261 agacctagta cttactgggt ctaaaccagc tgtaggttca tcaagtaata caatttcatt
    10321 ttccattgct ataactgatg ctattgccac tctttttttc tgtccatagc taagaaaatg
    10381 tactggcctg tctataaaat ctattccatt aacagctact agagctttgt ttattctctc
    10441 tttaattgtt ttttcatcca taccaatgtt tctcattgca aaggctatat catcatatac
    10501 ttttgaataa aaaatttgtt tttctgggtc ttggaacaca atacctacat tttttctaag
    10561 gttgtataaa cttctcttat catattttaa cttttcttct ttaaaaagaa tttctccaga
    10621 tgtaggcttt aatataccca ttaaattcat aaataatgtt gattttcctg aaccatttga
    10681 acctattata cctataacat ttccctttga aaaatccata ttaatgttta atagtgcatt
    10741 ggtatttttt tcatattgat acgttaaatt atttatttta aacatcatta tcccctacta
    10801 tgtgaaattt accatcatac aattttatgt ctaatgatat acacatatca tcataccttg
    10861 tcataagtct tttgtacagc atacttccaa gaatacctaa ggatttatat gaagttttta
    10921 aatttatata tccaaatctt agctcttgtg acttgtatat ttctgaaaat tcttctaaaa
    10981 atatgaatat aaacctatat attaacattg ataactcgac aaaagtgtca ggtaaatgaa
    11041 gttttttaaa aaagaatatt aattgattaa aaggtgttgt aagtatacaa aaatacacac
    11101 aagtcaaaca agacatcgct ctaaataaca catgaataga catgtcaata gacgcctttg
    11161 atattcctac attaaaacta aaaaattcaa aactatataa taaatctgct ttattaaaag
    11221 atatatttac aagagttatt cctatactaa gaaataaaaa gtacattggt attctaagta
    11281 attttaaata attttttaaa tctattccag ccacacaaac aaccaaaata ctcattaaaa
    11341 tcataattcc acttaaaata aatatatttt gtattaccat agaagcaatc agaaaaatta
    11401 ctcctatggc tacttttttg tttggattta ctttactcaa tctatttgta tatgcatatt
    11461 tatcaattat aagcatgatt aacttctctt tcctttttga agacctaata tgtatcctat
    11521 tacaccagcc cctaaagctg cttgtgcaga gaataataaa ctttctattt ctccacttgg
    11581 tggctcatat ggactgctaa accatggttt gtaatttgga tttatcttag taatttcact
    11641 ttcagcttga ccatctgctc ctccatattc agcacctgag ttgacaagta atgggaatat
    11701 aataagagct actactaata aaagtaataa tatgttagtt tttgtttttg ttttagcact
    11761 cattagcttt aactccttta tcattatttt ctagtatgct tttttctccg ttttctgtaa
    11821 ttaaattata tacaattaca gtaagtatac cttctgctat tgctattggt atttgagtca
    11881 taaagaaaat tcctaaaaat ttaattgctg aagccattac cccacctgat gggtctggga
    11941 aagccagtgc taattgtatt gaagttattg tatatgtggc taagtctcct attgctgctg
    12001 caagaaatac tggaatagca ttatttccat cttttttctt taaagcctta aatattagga
    12061 atgatacgat tggccctatt atcgccattg aaaaagcatt agctcctaaa gtagttattc
    12121 ctccatgtgc taataacaat gcttggaata ttaaaactat agttccaaga acaaacatta
    12181 cgcttggtcc aaacattatt gctccaagac ctacaccagt aggatgtgaa caacttcctg
    12241 ttactgaagg tatttttagt gctgataata caaacacaaa tgctccacat aaagctaata
    12301 atactttctt tttagggtct tgctttacta ttttacctat tgattttaat ccgaataaga
    12361 aaaatggtat aaatataact ccccatgcta tagaccattt tactggtaag taaccttcca
    12421 ttatatgcat tgcatttgat gtaactggag ttaaaatgat taacataagt gcagctataa
    12481 cccctaattt aatattctgt ttcatcttag tatccctctc tccctttcta ataattaaaa
    12541 cataataata catgtctgat ttatttaata aaataaaaaa gcctaactcg atggttaggc
    12601 ttctaatttt ccacaatcta aatacaaaca cataaaacaa acatatctgt ataattatta
    12661 taagaaactt tctccatccc cgaagaaatt actttaatat tttaggcagg tctcctgact
    12721 tagcttcatc ctactccttt tccttcccat gagatataaa aaatatcatc atagtggcta
    12781 acaaaagttt cgtctgcatc acagtagcgg gggctgtaaa ggaatttaac ctttttccct
    12841 attaatcttg cttaaagaac ctataatatt atatatttaa tttttaaatt attcttaaat
    12901 tattttctat ataaatcatt gtataccttt tctgtgaaaa tttctacatt ttttataaat
    12961 tttattcatt tttatatttt tatgtatata aattcttttt tgtaagttta ttaatttcta
    13021 attatcttct ttaaatacat tgaaactaca tcagaaaatc tagttatatc tcttgaatat
    13081 ataaaatcat ttccatatat taatctttca gcttcaagtt ctgattcttt tcctgtaaat
    13141 actcctaata ctaatatatt ttgctttcta gcttttctca cttcattggc tgtatctctc
    13201 aaaccaacta tacccctata agccttttct cctcttaatg ttctctcact actttttcct
    13261 atctttacat catttggtct tccatcgctg agaactatca ggattttatt ttcttcatct
    13321 ctatttatta gaccactaca tatagatttt attgccaaac catctctatt attaccagca
    13381 caaaaatatt caaatatatt ttcactattt tttatactat catcataatc tttaaatctt
    13441 tttaatattg tatagtctaa aaaactagaa aatcccatca ctctatttgg tatgccaact
    13501 tctgttagag catttgcaat tatgtaagct tgaattgcca catttccctg atttctactt
    13561 tgagagccac ttgcatccaa taatatatca ataacgtatt tccctttttc attttctata
    13621 tctttataaa atattttatt attattactt ctaccaactc tccaagctct atttgctaat
    13681 atagtaccac aatcagaata tactctactt ttagcatttt cttctattaa aattcttgac
    13741 agactttctt ttaatctctt tatttgctgt ctatacattc tagcatgttc tctaaatttt
    13801 cctaaattat tttctttttg tcttgttaca tattttagtt gaaatacatt attacactca
    13861 cttctaagaa ctccatctgt aaagtgtatt ctacaacctt catgaacatt tctacaattt
    13921 tgtgtctcaa tttttttaat ttcaccttta ctcaaataag tttttccata ataatgttct
    13981 atttttgaat atatcttatt tgcagtgtct tcatcaacat atataactcg gtctgctgaa
    14041 ttgctacatt tagaatcatc aaaatcccct acaccttcta caagcatgga tgaatatatt
    14101 gaatctatat cactttcaat aatattttct tcttcatcac tataaagttc ttcatacatg
    14161 aaatcagcaa atgtatcaaa atttacttct acactttcta cattattttc atcaataaga
    14221 ctattttctt ctccatttaa tatgtattca aaataagtta aatatataac atctaatgct
    14281 tttaaaatct ccataatatc ttgattggta tctatatttc ttatatcatt aactattctt
    14341 ctagtcatac tatctaatgc tgggtgcttg tccatactat aaagtacaaa tgtgtattta
    14401 attttatcca taatattatc attgtgtatt ttggtaaaac gtgaaacaat atctttataa
    14461 gcatttcttc ttatgtcttc tatccctggc ctatctttaa tgaccttttc ttcaactatt
    14521 gtatttaata tgacttgaat tagacccagt ataatatctt tgttgtatcc ttttcttatt
    14581 ctatttgtaa gatatttttt aactatatcc caatctacat actttcttct agcacctgca
    14641 attattgcga aatatagacc tatttctttt gaagagtaat ctttttctga tatatctata
    14701 ctttcattgt aatcaccagc tattgtccat gataggttat tagctctatt ttcaaattca
    14761 taatcatcat atatccattt cataatctca cctatattaa atatgactta ttggaaacac
    14821 atctatactc tcccatttat taggtattct agttttaatt acatctccaa ccatttcttt
    14881 ctcatataca tcaaaagtct ttcctgtaag tcccatatta attgcaagag ttggttttaa
    14941 tcctcttctt atagtcttaa gagatgccat aagaccacgt aaatcaatag ctttactaga
    15001 tatctctcca ttttgagact tcaattgtaa atctaaaaat attcctgcaa agtgtattaa
    15061 tttttcttca tcagcatctg aaaattcatt ttttaaaatc atcattaact tatcttcttc
    15121 tataggagga atatctatta ccataaatct agataccagt gcttcattaa gttcttttgt
    15181 tcctgcatat tcataattca tagtcccaat aaatcttgtt gctggatgta gattgactct
    15241 ctcataccca ggtacatcaa ttattcttct atagtctaaa gctgaatgta ccacaactat
    15301 agcatcattt tttgccatat taatttcatc aaatacccca aaacctccat taatagcaca
    15361 ctcataaact gagcctcttc taagtttaac ttcattgtct ataaatgtat ctgtacctat
    15421 aagtgttgaa ctgtctgtat ttatatgaaa tgatgtattc cactgaggtc tattaaatat
    15481 ttcccctaga ttatcagcaa gtaagttttt tcctgttgcc tttggtcccg ataataatat
    15541 attttcttct tctaaaatag ctgctataca catagacaag atatctttac cataaaaata
    15601 agttttagac tttgttactc tgtattctaa atctttttct aatccataat aatttctaaa
    15661 atacagtaca tcttcaataa gtttttcatc taccccttgc acccttagat tatctattat
    15721 atccatattt ttaccccttt tatataaata gtatttgttt catttcttat caccatttta
    15781 gcataaaagg atataagcta aaattgattt tttaatctcc ccattttaag taaaaccatg
    15841 caccatttta tcactacaaa attaaaagta ctacttactg atatttcttg attttgttcg
    15901 tctatacgta tataatatat gctgataaat tatgaggaaa ggatgattga aatatggaat
    15961 atatgtcttg cccagaagta gcaaaaaaat ggggtatttc tgaaagaaga gtacaaaaac
    16021 tatgtgaaga aagtcgcata ccaggagttt caaaactcgg gtatatgtgg ctgataccaa
    16081 agaacgcaga aaagcctgtt gatggtagaa caaaaagaag aaaggaagta caatatgaat
    16141 agcgttttga ttatagatga tgacaaagaa ctttgtgctc ttatgaaaaa atgtgttgag
    16201 caagaaaatt tatctgctat tactgcacat tgtggtgcag aaggtctaca tttggtcgat
    16261 aaaaatagac acacactctc tcttgtaatt ttggacataa tgatgccaga tatagacggt
    16321 tttcaagttc tgaaaaaaat tagagaaaca agtaatattc cagtaattat gcttactgct
    16381 aaaaatgacg aggatgataa agtttccgga ctacgtttag gtgctgacga ttacctaaca
    16441 aaaccgttca gcataaacga acttatggct cgtgtcaatt ctcttattcg tcggtatact
    16501 atgttaaacc ctgcttttat aacagacaca gattgcataa taataaatgg catgattatt
    16561 gacaagacaa accgaattgt ttctgtcaat aatattcctg ttcaacttac aagcaaagaa
    16621 tttgatttac ttgcttttct ggcttctaat aagggacggg tgtttaccaa aaaacagatt
    16681 tatacacaag tatgggaaga agaatatgct tttgacgata gtaacatcat gtctttcatc
    16741 agtaaattga gaaaaaagat tgaaccagac cccaaccatc cattttatat tttgacagtt
    16801 cgtggtgtcg gttatcgttt taataaggag gcttaacatg aatacaagtg tctatctgct
    16861 tttgatattg gctcttagcc tgattattat agcgtacctt gtttctgtcc tgctacgtgt
    16921 ccgaatacaa cttacactta taaaagatgc tttagaggat ataaaaaatg gaaaccttaa
    16981 tcgacgtatt ttaacagatg aaaacgacat tacaaaacaa atttgctatg atattaacga
    17041 aatagcaatc aatagccaat ttcaacttat tcagcaaaaa caatcagaac aagcctacaa
    17101 acagctaatg acaagtctct cccatgatgt aaaaactcca cttgcttctt tggtcggtta
    17161 tttggaagca gtagaaagta aaattgttgt aggagaggaa aaagacgagt atatccatgt
    17221 tgcgtctaat aaggctcatt acttaaagaa ttttgtagaa aatctatttg aatgggtaaa
    17281 attggattct aaggaacaag tttttcattt tgatattttt gacttgaatg aattatcccg
    17341 taatattata tctgattgga tacctgtttt agaaagcagt cattttgaat atgaatttga
    17401 tataccagaa atagagtact tcttacgtat agacgcaaat gcatataccc gtatcattaa
    17461 taacctatta caaaatatta ttacccatag tagtggcaac aaaatgacat tgagaatttt
    17521 tgaaaacaaa gagcaagctc aaattataat aacagataat gggaaaggta tatcttcaga
    17581 caatctccca catatttttg aaaggttgta ccaatgcgac cattcacgag cttcaaaggg
    17641 aaatggttta ggcttggcga ttgcaaagga acttattaac gctcataagg gaaatattac
    17701 cgccaacagc actcccggta tgggaacaga atttacaata ttgcttccta aagccctgta
    17761 aaagtacgag gctttcttac tctaaaacaa gataaaagca agattatggc aaggttttgg
    17821 caaggttcat gttttataat tctaattgta gttgattttg acgttgtatc aactatcttt
    17881 tgtagaaaca aatttagtaa aagtgaggaa attatatatg aatgattttg tgattgaaac
    17941 aaagcaactt acaaaaattt atgggaatca aacagtagtt gacaaagtca atctccatgt
    18001 aaaaaaaggc cgaatatatg gtctgttagg acgcaacgga gctgggaaaa ccacaattat
    18061 gaaaatgatt ttaggattga cttcaatcac gtatggcgag gtagatgtat ttggagagaa
    18121 tattaaggga cgtgaaaaac aagtatatcc ccgtatcggt gcaattatcg aaacgccagg
    18181 attttatcct aatctgacag gaacggagaa cttggagatt tttgcaaaac tgcgtggtac
    18241 agccgcccct aattctgtaa aaagagcatt ggaaattgta ggcttacctt ataaaaataa
    18301 aaagctgttt agtaaatatt cacttggcat gaaacagcga cttggtattg ccaacgctat
    18361 cctgcatgac ccagaactgt tagttttaga tgagccaacc aatggtcttg accctattgg
    18421 tattgcggag atgagaaagt ttattaggga cttgagcgtt gagcatggaa agacaattct
    18481 tatttccagt catattcttt cagaaatctc attgttggct gatgatattg gaattatcga
    18541 ccacggtgtt cttttggaag aaagtagtat gagtgaacta gaaaagaaaa acagtaaata
    18601 tatcctgcta caagtttcag atattccgaa gacatcactt attttagagc gtcaatttaa
    18661 cacgaaggat tattccgttc aagatgacta cacgttacac ctttataaca ctaatctgga
    18721 tatgggtgaa atcaataaag cacttgtaat gcagaatgta attgttatta gttctcaact
    18781 ttgtaatgat actttagagg actactttaa aaaaatcaca ggaggagagg gcattgctta
    18841 aacttattca aacagaattt cttaaactgc gtcgtaggaa acttatttgg ttgatgttgt
    18901 tgtctgctct cattatgccg tttttcgcac tactatactt caattatttt ggtaaaacag
    18961 gtgttgagcc aacaaagttt tataaatggt ctgcatttgg ttatacactt tggattattt
    19021 tgcccgttgt tttgggtata ttttgcacca tgcttataca cgaagaaaac cagtatgata
    19081 tgctcaaaca gctttggatt gtacctatta gtaaaatggg atactttttt agtaaatttt
    19141 tcgttgtttt gatttactct atttgtttta tgctgattac agccgttgct tctgtgttat
    19201 ttagtgtact ttctggatat gtcgtattta gctgggcaag cattttatat ttgttgaaaa
    19261 aatgtttaga aattggtgta atcactgctt ttgtaatgtt gccgattttg gcaatcgcta
    19321 catctcaaaa agggtacatt ctcccaattt gtatcacatt ggtatatgcc ttttccggtt
    19381 ttgttataat gacaataaat atgtatttac acccattatc cagtatggga gttattatca
    19441 tgagaaataa agacattcca ggtcttatat tttcacagga aattaatgtc ccattagcat
    19501 ttctttgtat ttgtatttgg agtattgtat cagtattgtt tgcaaacatt gcactaggaa
    19561 gaagaaagtg aggtgtagat aatgtggaaa ttactttggg ctgaatgtca aaaaattcgt
    19621 cgttcaaaaa ttgtttggat tacagttttt gcatccatta tgatagcagt catagttttt
    19681 gcaggagggc aggatgtata taatgaccca gatttacatt atggcttaaa gagcgtttat
    19741 gacggttcac ggtatataga caatgcaggt tggtatatgg acgcagtaca accttggtca
    19801 actttttttg ttcttcctgc tgtcattgcc ctattaggaa gctatattat ttgtcgtgaa
    19861 gaagaggagg acactattaa atccctgcgt cttattccca taaatgaaac aaaattaacc
    19921 atagcaaaaa tgattattac ttttatattc agtatacttc tttatttgtt actctttact
    19981 ataacatttt tgactgaatc cgttttacat ttttccgatt tatcgacaaa actggtttta
    20041 agttgcatga aagagtattt tcttgatggc ataggtgtat tctttgcaat ttctcccatt
    20101 attgccttgg tatcgcgcat gaaaaaagga tattggcttg cattagtatt cacagaagtt
    20161 tattctattg ctggactatt tgcgggtatg tcaaatgtct tacaaacctt ttttcctatt
    20221 gcggcaatat ttaatatttc tggatactat ataactactg gagaaaaaat tatggggagt
    20281 atcataagtt tattactttg tgcctgcctt tctgcattta tactgaaagg gctaaaacac
    20341 aatgaaaaaa attgaaatat gtatatcaaa atgaaaaaga taaaaaaact gattattcta
    20401 attgtattct tgtttctttt tggcatgtta cactgttata ctgaatggaa attcaacaat
    20461 gacccaccta aaggggtaat agaaagaata aatgagtgga ctgataagat tggactggat
    20521 tagtaactta tgttaattat agtgaatgta atattagcaa gttattgttt cgtattttaa
    20581 tactataact ttaaatattt ggttttaatc ttccaaacga atataaaaaa actgtcacag
    20641 aggcaacata tttttgaata tttataaagt ggtagttttg attttcaaag ctgccgcctt
    20701 ttcttctata taagcaaact tatcctcaac aaagtgtaaa acatggtaca ttttaagaaa
    20761 aaagtaccac agacacaagc aataaaagaa acgccgagaa cctacaaaaa cgtagtgctt
    20821 atgcagattt acgacgcctt ctattttctc aaccgtctta atgaccttgt acaaaagaaa
    20881 tttttctaat attttttata atcttatctt gtgatgtttt ttatgtataa atgacttaat
    20941 aacaaatcca ataaaaatta tggatattgg tataattaga acgaggaatt tataatcatt
    21001 ataatgtgac attattctta cccaactctc agaaaaaata tatcctatac tgattaatga
    21061 agtattccat atggttattc ccacgataga tgatattaca aaagttataa tattttgacc
    21121 tgctatacct gctataaaag atatgtatgt tctacataaa gggattaatc tacatacaca
    21181 aacagaaata gaagagtatt tattaaaata atcttgagat gcaaatattc ctttctttgc
    21241 ctttggacat atctctatta ttttatttat gatagattta cctccccaat ttcccactgt
    21301 ataacaaaat atcgaaccaa gtatacctgc aattatactt aaaattaaaa ttgtcagaaa
    21361 tccaaaatga tttcttgcag caattgctcc acataaaggt aaaacaactt cgctaggtaa
    21421 tggaaaacaa gcatactcaa tcataattaa tacaaataca gagattaatc catattgtaa
    21481 aataaaacta ttgattacag acacttactt atctatctcc tttcttattt aaaggtttta
    21541 agggtacatg ttctaaggat tctcacccta tatttaaaga atcaatctct ttaaactatt
    21601 atatgtattt acacaaaaca tttataacat actttaaaaa tagtacctta aaataagaaa
    21661 cttaataaat aaaaaaagat tacgtggcta cgtcctactc tcccaggcag tttcccacca
    21721 agtaccatca gcgctagaga gcttaacttc tgtgttcgga atgggaacag gtgtatcctc
    21781 tctgctattg taaccacata atctttatgc ttaatatttc tattaagttt ttaataagtt
    21841 tatagaacct agtaatacta gtttctttag agttagcact ctaaaaact
//
LOCUS       DAGSHT010000047        21794 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.048, whole genome shotgun sequence.
ACCESSION   DAGSHT010000047 DAGSHT010000000
VERSION     DAGSHT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.048"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..491)
                     /locus_tag="MJB72_001588"
     CDS             complement(<1..491)
                     /locus_tag="MJB72_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY8262706.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSV"
     gene            complement(531..878)
                     /locus_tag="MJB72_001589"
     CDS             complement(531..878)
                     /locus_tag="MJB72_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262707.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1096..2052)
                     /locus_tag="MJB72_001590"
     CDS             complement(1096..2052)
                     /locus_tag="MJB72_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBY8262708.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2497..2865
                     /locus_tag="MJB72_001591"
     CDS             2497..2865
                     /locus_tag="MJB72_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBY8262709.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            3290..3535
                     /locus_tag="MJB72_001592"
     CDS             3290..3535
                     /locus_tag="MJB72_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262710.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            3634..3897
                     /locus_tag="MJB72_001593"
     CDS             3634..3897
                     /locus_tag="MJB72_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBY8262711.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(4182..5129)
                     /locus_tag="MJB72_001594"
     CDS             complement(4182..5129)
                     /locus_tag="MJB72_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBY8262712.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5132..6763)
                     /locus_tag="MJB72_001595"
     CDS             complement(5132..6763)
                     /locus_tag="MJB72_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY8262713.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            6893..7810
                     /locus_tag="MJB72_001596"
     CDS             6893..7810
                     /locus_tag="MJB72_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8262714.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(7867..8448)
                     /locus_tag="MJB72_001597"
     CDS             complement(7867..8448)
                     /locus_tag="MJB72_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY8262715.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(8488..9054)
                     /locus_tag="MJB72_001598"
     CDS             complement(8488..9054)
                     /locus_tag="MJB72_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY8262716.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(9510..10370)
                     /locus_tag="MJB72_001599"
     CDS             complement(9510..10370)
                     /locus_tag="MJB72_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY8262717.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            10519..11409
                     /locus_tag="MJB72_001600"
     CDS             10519..11409
                     /locus_tag="MJB72_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8262718.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            11627..13381
                     /locus_tag="MJB72_001601"
     CDS             11627..13381
                     /locus_tag="MJB72_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBY8262719.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            13765..14751
                     /locus_tag="MJB72_001602"
     CDS             13765..14751
                     /locus_tag="MJB72_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY8262720.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            14920..15068
                     /locus_tag="MJB72_001603"
                     /pseudo
     CDS             14920..15068
                     /locus_tag="MJB72_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            15087..16568
                     /locus_tag="MJB72_001604"
     CDS             15087..16568
                     /locus_tag="MJB72_001604"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBY8262721.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            16594..17565
                     /locus_tag="MJB72_001605"
     CDS             16594..17565
                     /locus_tag="MJB72_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBY8262722.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(17821..18423)
                     /locus_tag="MJB72_001606"
     CDS             complement(17821..18423)
                     /locus_tag="MJB72_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8262723.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            18704..19915
                     /locus_tag="MJB72_001607"
     CDS             18704..19915
                     /locus_tag="MJB72_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBY8262724.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            19983..20180
                     /locus_tag="MJB72_001608"
     CDS             19983..20180
                     /locus_tag="MJB72_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBY8262725.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            20214..21185
                     /locus_tag="MJB72_001609"
     CDS             20214..21185
                     /locus_tag="MJB72_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8262726.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            21372..21749
                     /locus_tag="MJB72_001610"
     CDS             21372..21749
                     /locus_tag="MJB72_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8262727.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         8361 a         2696 c         3209 g         7528 t
ORIGIN      
        1 tctacagaaa aaattttttt catattcagt aaagatattt gcttagataa gattctatta
       61 ttgtatgtaa tatgttgaga tataaaaggt tgaatgtcaa tcatcatatt aaataactct
      121 aagcatttac ctgtatattt atatactcta aatgaagtta gaaggccagt tatatcaaaa
      181 tcaccccaat tatatattgt aacatcatca aatttacaaa accatttata aaaatcatcc
      241 ataacatata aaagattagg agcattattg atatctagtt ggttcaattt ggttaaattt
      301 ttacatttat ttgaaagtat tttattgtat tttggcttaa ctaaagagta ataattatct
      361 attgtattaa attcgttatc aactaaaact ccaccaatag aaattaattc cgcaccatta
      421 tattcttttg gtttattttt attatcatct agtatattaa attcaaagtc tataaataac
      481 ttgtatttca taaatctaaa gcacatcctc aggtaaatta tttattgagc ctaagcatca
      541 taagaatcaa tagatataat gactctaaac atcaaaggtt cattacaact tttatttatt
      601 tcataattta attcaaataa atcttttaag atataattta taagtattct ttcccaaccc
      661 atttttaaat taactttaga gtctattaat atattttcta tctttttaaa tatttctata
      721 tttgctggta ttatactgag ctcaatatta cttttaaacg ggttagactg taaatctgtt
      781 ataagtatat tttttaattc ctctgtaagt tcagaaatta aactatcacc tatgtatttt
      841 tttatgattt cctctctaat ttcttttaaa tttagcaata ttagtacctg ctacttacat
      901 agtagctttt cctttccttt taatactatt attcattttt gaatcttatt tagatatatt
      961 ttaagattaa aagtctttag ctatataatt ttttacccat ttttaactta tattaacata
     1021 ttacaggctt atatctccac cctgtttaca ttgaggatgg agtatattgc ctattttgat
     1081 aaaaatgata ggttgctatt tgcctattcc tttttctgat aatttttcta acatatcttt
     1141 ggtcatttta tctaaatcat aactaaactt aaagttccat tcttttactg cactactact
     1201 gtctagagag ttaggccaag aatcagctat actttgtctt acagggtcta catcatattc
     1261 tatcacaaaa tcaggaatat atttttttat agaatcagct atttcctcag gagaaaagct
     1321 catagcagta atattaaaag catttctatg gatgagttta tcagctggag cttcgattaa
     1381 atccactatt gattgaagtg catcaggcat atacatcata tccatttttg taccttctgc
     1441 tatgtaagat ttatatctct tattttttaa tgcctcataa tatatatcaa ctgcataatc
     1501 tgtagttcct ccaccaggag gcgtaacata agaaataaga cctggaaatc taacacctct
     1561 agtgtctact ccaaattttg agtgataata atcacatagt aattctccag aaactttagt
     1621 cacaccatac atagtattag gtctttgtaa tgtatcttga ggagtcatat tttttggaga
     1681 attttcacca aaagcagcaa ttgaactagg tgtaaaaaat ttacaatcca attctttggc
     1741 tatttcaaga ccatttatta atccattcat atttaagttc caagcttcta gaggtttact
     1801 ttcagcaact gcagaaagta aagatgctag atgaattata gtatctacat catatttttt
     1861 tgcaatttca aaaaaacgat tcttatctgt aacatctaat atctcaaata taccagattc
     1921 acatactgga ttaccttctt ttactctcct tgaactagcg attacatttt gttctccata
     1981 ctcatttcta agctttattg ttaattctga acctatttgt cctaatgcac ctgttataag
     2041 tatttttttc aattttacaa cctcctaagg gtttaattta tgtagtacta cttattttta
     2101 ttttagcaaa tcttttgttt aaatacaaag aattaaagat aataatatac gacttaattg
     2161 aatgaagtgc agtaagtgcc taaaaattta aagctaatat ataatggtat attgtataac
     2221 aataaaagag tagattaaat tattatatat agattgtgtg actaagtcac agaaaataga
     2281 gttaatttaa tatataataa aagcatatca agtaaagctt taaattaaag taactttata
     2341 aataaaaagt aatataaatt gataaaatta taaatttatt tatagtgaat atgggttgga
     2401 ttttatagat acaaaaatag tatatatgat atgtagacca ttagcatgtt taaagtagca
     2461 aaacatataa aataaaattt gaaaggaagt ataaaaatga caagaccatc aatatatcat
     2521 agccaaggat acacttgtgc agaggcatta ataaagtctt ataatgagga acataacaca
     2581 gatataccaa tctcaatagg aagtggtatg ggggttggaa tgaatgttgg aagtgtttgt
     2641 ggagctgtaa atgctgctgt tgttataata gggtatttaa atgggcgaaa tagtaattta
     2701 gatgaaaata tagcaaggaa gtattcaaga gagctattga atagagtaag aaataaatac
     2761 agctcagaaa tatgtgtatc tcttaaaaaa aataaagtaa cttgtgaaga aataataaat
     2821 ttcacatatg atgctctaaa tgatatgcta aaaaatcaaa aataattcta agaaacaagt
     2881 attttacatt cgatagaata cttgtttttt tatgatattt ttaataagta ttagtttatt
     2941 ataattttta aatcaatttt attatatttt aattgaaaca atctactttg aaaattacta
     3001 actgacaaaa gaatatgatt atagtataga aaaaatgtaa attatttaat attaagtgag
     3061 agtatatgtt tacctatata aaatcgtata aattaatggc taaatttata aaatataaag
     3121 aaatatatag gaaaacaaaa gaattaatta gtataatata aagtttaaaa gttaaaattt
     3181 atataattta tttataaagt aaagaaaaaa tagaaatttg caataaaata aaaagtttat
     3241 gacataatat ggtataatta gtaaaaatat agagattgga ggaattgctt tgtttataga
     3301 tgaagaatta gaaggttata tattaacatg taaaatatct gaagacttta aaaatatacc
     3361 tgaatatagt gatgaagagt tttatgttac agtctataaa gatgaaagtt ctgactctgg
     3421 gtactatgct ttattagaaa ataaagaaga aagagttgta tgggatggag aagttgttgc
     3481 caataatatt tttaataacc tttggattgt agtaaataag gttaaaactg gataatagtc
     3541 taattatata gtatttgtaa tattttaaat gaatttttac atatttgaga tgagtaaata
     3601 acaaagatta ttcgatactg gaggagtaat tcaatggaat atagttatag taagatgaac
     3661 ttaaaaaaag gggatattgt agaggtaaat ttagagaaac aagccaatgt aatattgtta
     3721 gaccatataa attatgtgaa atttaaaaat caaagaaatt atgattacta tggtggattt
     3781 gcaaagaaaa atccatgtag aatgagagtt cctaatactg gaacatggta tctagtagta
     3841 aatcaagatg gaaatagtgg aatagtaaac ttttcaatca atacaattca aaattaattt
     3901 tagaatattg gatattctta atttttgtac attttatata gttaaaataa aattaataag
     3961 caattattat aaaataatgt gggtgcctca aaatgaattt tataattcat gagacactct
     4021 tttttatatg aactcaaata tactttatca tttcaacaat gaaaaagagg cgtatctgtg
     4081 ttttgagata gcctcattta tcatatatca tatatagaat tttaattatt cttaactaaa
     4141 attttttcca taggttatct gtacaattaa aatatttatt gttagcatac agctccacct
     4201 gtaaggtgta aaagagcctc tagtatctct tcttttcttt ccaaaacttc atctgataaa
     4261 agcatttcta taaatttatc agcaccgatt ctatcgatag ttattccaaa acgttcacct
     4321 gtgttaccct gttctctaaa taaaagaatt gctttttcaa ttaaagtcat aagttcctct
     4381 tttgaaaata atctatcaat ctgtgttcca ggacgaactg attttcccca tttccctcct
     4441 atatagactt tataaccaga ttctttttct tcaatactat caaaattaca actttctata
     4501 cacttaccac agttattaca tagattacta tctatttcaa gtttaccttt atctgttaat
     4561 ttagctgctt taacaggaca gacctctaca acagcacatt ttttacaacc aacacaaagc
     4621 tctgaatcat agtcaggtac acgttgacca acaattccaa gatcgtttag gtcaggttta
     4681 atacagttat tagggcatcc accaacacca attttgaact tatgaggtag ttttacatca
     4741 taccaatttt tgtaaaattc ttcatgtatt tcagcagcaa gtgcctgtgt attagctaga
     4801 ccatgaacac aaacagtacc tttacaagcc acaacaggac gaacccttga tccagttcca
     4861 cctgttatta gattttcgct agcaataaat tcacttagag cttcaatatc ttcaaatttt
     4921 acaccaggta attctactgt tagtctagta gtaaatgcta actgaccgtt tccgaatttt
     4981 tcagcagctt ctgcaatttt ttttgcttgt gatgcattta ttacaccatt tacagttatt
     5041 atacgtgctg caaaatgttc tccatccctg cttggtagaa aacctctacc ttttacttcc
     5101 tttttttgtt ctgctgtaac attactcata tttactcctc cattacctta agatttggat
     5161 aaaatagtat tccaccttca agaactttcg tatttgtata gccaaagcgt cttaagcgat
     5221 tttgcgccat atagctattt ttacctttag cacatataag aagaattttt tcatccttag
     5281 caagaccttc aatttctcca ttaatttcac caacaggaat ataacgtaaa ctaggaatag
     5341 aaggagcttt tgaaacatca agtttagtcc aactgtcaaa atcctcaatt tcgtcaagta
     5401 aaacactatc aagttctcca ttaagtttat tttgtaaaac atttactgcc actacaaaag
     5461 ggtgaatagc tgttgaaaaa ggtggtgcat atgcaaggtc catactttct aatgaataaa
     5521 ggtcagcgcc taaagttatt gcagtagcag cgatatctac tattttatca accgctccag
     5581 gtcccataac ttgaacacct aataattttc tagtattttt ttcagcaacc attcttataa
     5641 taaaattaga tgcacctgga taataatgag ctttatcatc agtagctatg gttacagagc
     5701 aaacattata accttctttt tcagctattt ctttacttag acctgttttt cctgcattga
     5761 gctcaggtaa ttttactact gttgttccaa gaacaccatt gtaagtttta gattttccag
     5821 atatattttg tgcgcaaatt ctaccctcat gatttgcaga tgaccccata ggtgaccatg
     5881 taggtttatt tgttaaagaa ttttttacaa aagcacagtc tccaactaca tatatatctt
     5941 tgtcatttgt aagcatatat tcatctgcaa taattgtatt attaggggca aattctaaac
     6001 ctgtgtcttt tagaaaacct gtgttaggac ggattccaac tgacataacc actaaatctg
     6061 ttttaatagc tctattttta gtacgaagtt tttcaacttt attttcacct tcaataccta
     6121 caacctggtc acctgtaaaa atcattatgc ctttatcagc catatgattt tcaacgtatt
     6181 cagcaaaatc agtatcaaat ccaggcataa catgttcagc catatcaaca acacttacac
     6241 gtatacccat tgcagaaaga ttttcagcaa tttcaagacc aatgaaacct ccaccaataa
     6301 caacagctct cttaataccg ttttcaatag aatctcttag ttttatagca tcatttggag
     6361 tacgcataaa gaatactcca ggtaaatcta ttccaggtat tggtggtttt attgggtctg
     6421 cccctgtagc aattacaagt ttgtcatagc ttagattttc tttttctcct gttgaaagcg
     6481 ttttcaaagt tacttgtttt gcatttctat caatagatgt aacttcaact ccacaacgaa
     6541 cttctgctcc tgttagttta gaaaaacttt caggtgtatt tacgattaaa gatgatttat
     6601 cttcaattat cttaccaacg taatatggaa gaccacaacc tgcataagaa atatcatcac
     6661 ctttatttag tattgtaaca gagcaattat cacccatttc acgttttatt tttgcagcag
     6721 tttttgttcc agcagcaact ccacctataa ttaatacttt cacaatatat cacactccaa
     6781 tctactttat aactctataa taaaattata cttcttgaca atatatttgt aaaatactaa
     6841 aatattaata aaatcataag catttactaa tgtttgctaa ggttgtgata atatgacaat
     6901 tcgtagtctt gaaatctttg ttaaggttgc tgaatgtggt aaaatgagtg aagtagcaag
     6961 aaatatgtat attacccagt catcagttag ccaagctatt tcagaaatag aaaaagagta
     7021 tggtgttaag ctatttgata gaatttcaaa aaaattatat ttaactgagg cagggaaaaa
     7081 acttcttgga tatgggaggc atcttttagc tgtaaatgaa gaaatgaatg attgtatgaa
     7141 gcattgtgca aaaaatatcc gtataagagt tggggcaaca gtaactgttg gtacatgtgt
     7201 aataagtcca ataatgttag aattatacaa ggtaaatcct cttatagaac ctgaagtatt
     7261 tgtagaagat acaagactga ttgcaaaaaa attacttaat agtgaattag atattgcgat
     7321 tgtagaagga aaaattaagc acccagatat tgttacaaaa agcattatta atgataatct
     7381 tgtacttata tgttcacaca agcatgagtt ttataaaaga gactctataa aagtttcaga
     7441 actttcaaat caaccattaa ttatgcgtga attgggaagt ggtacaagag cacaattaga
     7501 aaagcaatta aaagaattaa aaatcccaat gaatattaga tggtcatgtt ataactctga
     7561 agctatatta agagcagttg tagataattt tggcattgca gttatatccg aattacttat
     7621 tgaggattat ttaaaaaagc atcttttgtg ggcttgtgat attgaaggta ttaatttaca
     7681 taggacattt gatattgcat atcatagaag caagtttttt acagaaaata tttcagcatt
     7741 ttttgatatt tcagtagaat atgggaaaaa acaagcgaga aagaagagta gaagtgtaaa
     7801 cagtttataa tatatctaaa taaaaataag agtatatcaa tatactctta tttttattta
     7861 gatatattag cctatattaa cgccaaaatt tttacacaat cctgcaagtc catcctcaaa
     7921 tccagaacct aaagcattaa atttccactc tccattatgt ctatatagtt ctgccacaac
     7981 tattgctgtt tctatgctaa aatcttcacc caattcatat ttaataagtt cctcattagt
     8041 ttcttcatta tatattctta tatatgaatt ttcaacttgt ccgaaatttt gtcttcttgt
     8101 aattgcttca tttatagtaa cactaaatga tattttagca atattttgag gtattttaga
     8161 taagtctaca tttatttgtt catcatcacc atcaccaaca cctgttctat tatcacccat
     8221 atattcaaca gctccagaag cgtgttttaa attattatag aatataaagt ctccatcatt
     8281 cgttactttt ccatcagttc cagttaaaaa tgcagaggca tctaaatcga aatcaaagcc
     8341 accatcatat ttattaatat cccatcctag tcctactatt acttttttta gaccagggtt
     8401 actttttgtt aaacttactt tttgtccttt tgctaaagtt atacccattt aaaccaactc
     8461 ctcattataa attttttatt gtaattatta cttatatgta ttaattaatt cacgaagctc
     8521 ttcattttga ccaattccta aagcattaaa tttccattct tcatttctac gataaatttc
     8581 tcctgccaaa actgttactt taccagaaaa gtcttcttga agattatatt taaataattc
     8641 ttgcttggaa tttgaatcaa ttaatcttat aaaagagttg tttatttgac caaaatcctg
     8701 gtctcttttt ttagctttgt atatagaaac tccaaaaact attttatgta catattcagg
     8761 tatgtcttct aaattaacaa ttatttgttc atcatcacct tcaccttttc cagttaagtt
     8821 atctccagtg tgtataatag ctttagaagg gtgtttaaga tttccaaaat aaaccaagtc
     8881 acctctaaca ttcattttat cttggttagt caatgctatt gcaaatgcat ctaggtcata
     8941 atttttttta cccaatttat tcatatccca tcctaagcct actactagat tttttacagg
     9001 attattatct tttactaagc taactctttg tcctttttgt aaactaacac tcattttaag
     9061 ttctcctttt tatattttta gttttattga tggtatcaag tataagaacc atattagcta
     9121 tattatatac gtatttaggc taagttggct atatttataa attatttatt atataactac
     9181 atgaatattt tataatttaa acatattgac attatatgct ttaaattatt ttgtagtttt
     9241 ataatattta aatttgtatt tgattgttat aaagtaaagt aagtaataaa gttagactca
     9301 ataattctaa aatttctaag catactatct aaatgagaac ggatttggaa atacttgtat
     9361 aaaacaataa ttgatacatt taatttttat cttaaaaaca acaattataa tatataaata
     9421 gcaagtataa tatataaata gcaagtataa tataacatat aaaaagttga atttgtatat
     9481 gttaattata cttgctattt atttgttagt tattctattt ttttaaatcc attacctaaa
     9541 gcttcatgta tatctgtaat tataataaaa gcattagggt cgacattttt tactaatttt
     9601 ttcaattcga caacttcttt cttttcaact actactaaaa gtacattttt attttctcca
     9661 gtatatgcac cttctccttt tagaatagtt gcacctctat ctaaatcttc cattattttt
     9721 ttacttatat cttttgaaag gtcagatata atagtgaatg atttggaata attaaatcct
     9781 tcaataagca agtcactaac ttttactaaa atgtataaag ccacagttga ataaagtgca
     9841 atttcaacct gtctatttac tatacctgat aaaacaacta caacgcagtc tacaataccc
     9901 attaaaacag gtatactaaa tgtaggaaaa tatttattta gtaggagtgc cattaaatca
     9961 gttccacctg tagagccatt tattctaaaa ataagtcctt gaccaactcc cattaaaata
    10021 gaaccagata taattgcaag atacatatca tttgttatat ctaatgtagc catatttgca
    10081 gtaagcttaa gtgctatggt aagaaaaatt attcctaaaa ccgttttaaa acaatctttt
    10141 tttgaaagta ttttgtaagc aaatatgaat aatggaacat ttaaaagtaa attgacaatc
    10201 caaagttcta caccgaataa tgaatttaaa acaactccaa gtcctgttat accaccagca
    10261 gcgattgcat gtggattaaa aaataagtta agagaaatgg ccattaatat acagcctaca
    10321 attaatatta aaatttctga aaaagttatt ctatcttttt tcaataacat cactccttat
    10381 atatagatta taatatatat tatatagttt ttccaatttt taataaaaga aataaaaata
    10441 tattataaaa acaatttgat tggaattttc agaaaattaa tgctataatt aaaagtaaat
    10501 ttaaagggag gttataaaat ggctaaatta actctagaac atataaaagg aaatacatat
    10561 tatattccac tccctacaat agtaggtgta tacgtagatg gtaaggatgc aattttaatt
    10621 gacagtggaa ataataagga tacagctaga caagttttaa gattattaga agaacataat
    10681 ttaaatccta aattaataat aaacactcat tcaaatgcag accatatagg aggaaatgca
    10741 tatttgaaga atcaaacaaa atgcaagatt gctacgacta aaattgaagg atattttaca
    10801 gaaaatccaa tattagaatc agcattttta tatggtggct atccaagtaa agcactgaag
    10861 aataaatttt tattagcaaa ggaaagtgaa gttgattata ttatcccttc aaatggcaaa
    10921 attgtagata cagaacttga agcgatttct cttccaggtc attattttga aatgattggt
    10981 gttagaactc cagataatgt tttgtttgtt gcagatagtc ttacaccaga gaatataatt
    11041 actaaatatc attttttctt cctactggat atagattcac agtttaagac actagaaaaa
    11101 ttgagaatgt tagaggctga tttctttgtt ccaagtcact ctgtcaaaac tacagatata
    11161 aaaagtttaa ttaatattaa taagaagaag atggaagaaa taatagataa tattaaaaag
    11221 gtttgttatg agccagttat gattgatgaa gttattgaaa aaatgtgtga tttgtacaat
    11281 gtcaagcttg atgctaatca atatgtgttg gttggaagta caatacgttc ttatataact
    11341 tatttatatg agaataatat ggttgaatat atatttgatg gtggtaagat gatgataaaa
    11401 gctgtgtaaa tagtaataat tttcaaatta tatgatgaaa ttgttttagt atgactgtta
    11461 tttgaaaagt aatataaaaa aatttatata aataagctaa gatgattgat ataaagttac
    11521 tgataatttt aaggtaatat atacgttact ataatattat gttattattt gtaaataagg
    11581 ggataattta taagcaaatt aaagacctta atttacagga ggaatagtgt atattatgaa
    11641 taaaaaattc aaaaaattca gtaaaaaact aagtatatta attttagttt tgattatgtt
    11701 tctttttaat aatttaactg caaatgcaca gattaaagaa gattcaataa taggaattga
    11761 tagatatgaa acagcaggac ttatagcaga tagacaaact tatgatactg taatattagt
    11821 taatggagat aaaaatttat ctgatgggct ttcatcaagt ggattagctg gagctattaa
    11881 cgcaccaata cttttgacta aaaaaagtga aataccaaaa gctactttaa atagactaga
    11941 taataagagt ttaaatgttg taaaaaaagt atatataatt ggtggttata atactattga
    12001 aaattcagta gaaaaagata taaaaagaaa aggtattcaa gtggaaagaa taaatggtaa
    12061 caatagaatt gaaactagtt ataatgtagc taaaaaaata aatgaagtaa gtagtgtaaa
    12121 agaggtaatg cttacaaatg gatttgtagg agaggctgat gctatgagta tagcacctgt
    12181 agttgcaaaa aataaaggag ctataatact tacagatggg aaatcagttc catttaagac
    12241 tgaaagttta aatgtttacg ctataggtgg caaatctgcg ataaatgagg atttagtaaa
    12301 tgaaactaaa gcaactagaa tcggaggaaa tgatagattt gagaccaata aaaaggtaat
    12361 ggaaaagttt tataatggag cgacagattt ttatataaca aatggatatc aattagtaga
    12421 tgcactaaca ttatctcctt tagccaaaga aaaaccaatc gttttagttg ctgatggaag
    12481 taataaaggt attttaaaag gagctaaaag tataacaaaa gttggaggta ttgatgccaa
    12541 tatatataaa cagtgtttag atatgattaa atataataat atggatatga ctccaaatat
    12601 tttaaaacac ttaacttcag ttgaaggaaa tgaagtttct gaaatactta tagaatcaga
    12661 taatttaggt gtggttgttg aaaagacgaa taccaataaa tttgagtttg attacgtaag
    12721 tgtaactaat gaaaaaaatt gtactttttc tgtatataaa gaaaattctt ctaataatat
    12781 aaaaaatgga aagctagttg taagtgctaa gaaaaaaata gataagaaag atagtgattt
    12841 gcaagatatg aatggcgaca atatgataaa tgcaaataaa gataggatgg taaatgtgat
    12901 aaaaattgga gttcccgata aaatgtattc taattttaaa gtagatgttg aaagtggttc
    12961 agtagaactt tataatatta aaggaggagc tactgttgat gtcaatgacg ggattgctaa
    13021 cattgttgat aacaatgtaa caaatccttt caatataaat actaatagtg ggatttcagg
    13081 tgtaacagct gaaactatat catctgaaat aaaatttaga gcaaacaatg gaattgtaaa
    13141 tgtaacagct acgaatataa atggcaatat aagtttattt ggaagcgata ataaaggtac
    13201 ctttgatgga atatttaaat taaatctgaa aaaagaacca tcaaatttac acttgaagtt
    13261 aattggaaat gggataaata aattgccaga aggttggtct aaagattata ttttaggaaa
    13321 tggacaacca attattgaag ttaaaaataa tggcataaat aatatatcaa ttggagaata
    13381 aaaacatatg aaaataaagt ttatatagtt ttaaaaatta tagaatttta aaatatttat
    13441 atatattctt taaataaaaa attatttttt aagtgataat taagtgttta tcgataaggc
    13501 tatggttgta aaaatcatag ccttattttt atattataaa taaattagag tatttatcaa
    13561 ttcatagtag ataaaattga aattaaatat aacaacaaaa aatgaaaatt acaattaatt
    13621 taatgttaaa gactcaatta tttggatgtg gaatggatag tacaagtagt atataaaaag
    13681 tattgactgg acaaataact atattttatt ttaaatatat aatataatgt aataaaatat
    13741 atcaaatgta gaaggtgata acctatgaaa aaaataacaa taaatgatat tgctaacttg
    13801 gcaggagtat ctaaaagtac tgtatcaaga tatctaaata ataaagatat cagtgattct
    13861 actaaagaga aaataaagac aataatagat gaatatggat atgagccgaa tgcatttgct
    13921 caaagtttga gagctaaaaa aacatacttt attggtatta taacaccgtg tttagattct
    13981 tttgtaaaat ctaaaattat gatggcaata gatgaagaac tcaaagaatt aaaatataca
    14041 tcactaatta ttaatactaa tagaaaaata aggtcagaaa tagatagtat atcaaaatta
    14101 gctagtctaa aagttgatgg aataatacta attggtaccg aaataactaa agaacataag
    14161 aatgaaattg aaaagttaga tataccgata gttgtagttg gacaaaaagt tgatggtatc
    14221 aatagcatag tgaatgatga ttatggagca ggatataaaa tgggtcaata tattgctaat
    14281 aaaggttaca aaaacattgt atatttaggt gttgatgaaa gtgatatatc agttggttta
    14341 aatagaaaaa atggagtact taatgggtta aaagataaag ggtatgatgc aaaagtattt
    14401 tatactgact ttgaccaaga aacgtctata caaagaagtg gagaaatgct agaaagtgaa
    14461 aatccagata taataatatg tgcaactgat aatatagcaa tagcaacaat gaaagaaatc
    14521 aataagagag gtaaaaatat accacaagat atatctgtag ctggatttgg gggatatgat
    14581 gtattgtcta ttataagccc taaacttaca acaataaagt ttgaaaataa aaatgcaggt
    14641 aaagtagcag caaatacgat agtaaattta atacaagaaa gaaaagaacc actattaaag
    14701 gagattaaat ttgaattaat agaaggtgaa agtacaataa ataaaaatta agatacacat
    14761 tacatatatt agtaatgtgt atttttttac taaaaaaact ataattctaa aaattaggaa
    14821 aacttttttg aaagaaatat tgcatctgaa ataaatttat gctaatcttt aattaaaccg
    14881 gtttcgaaac cggtttaatt aaaccgatag aatagcaaaa tgagtgcaaa aataactgat
    14941 tgtaacaaag tatcaaagta attattagag tacataggaa taaacaaaat ataaaagatg
    15001 tagcacattg tgtaataaga ctaagaattg ttttagatag caataataaa gcaaatataa
    15061 aattataaaa gaaaaggaat aaaaatatgt acaaagagcc taaatataga accatattag
    15121 aatcgacaaa agaagaatta aataaattaa gaaatatatc tttaaatgat aaatataaac
    15181 cactatttca tatacatcca caacatggac tacttaatga cccaaatgga ttagcatatt
    15241 ataatggtaa gtatcatgta ttttatcaat ggtatccata tgatgcaact catggaatga
    15301 agcattgggc ttatgtaagt tctgatgatt ttgtaaactg gaatagagaa gatgtagcat
    15361 taataccaat cgaaagttat gaatctcatg gagcatattc aggtaatgcg atagaggtag
    15421 atggaaaatt gcatatgtat tatacaggga atataaaata tagtgcagaa gatagatatg
    15481 catatcaaaa cttagctata atgaacaagg atggtaaaat aactaaatat gaaaataatc
    15541 caatagtaag tgaaatacca aaaggatata caggacatgt aagagaccca aaagtattta
    15601 aaagaaagga taaatacttt atgttacttg gagcacaaac aagtgataaa aaaggagcta
    15661 taatagtata tgaatctaaa aactctatag attggaattt taaaggagaa ttaaatgtaa
    15721 aaaatataga tgaagatttt ggttacatgt gggaatgtcc agattatatt aatatagatg
    15781 aaaaagatat attaatattt tcaccacaag gagtagaacc taaaggattt gattatcaaa
    15841 atatatataa tgtagtatat gctattggta atatggattt agataattta acatttgaaa
    15901 tagatacaat gaaagaactg gaaaaaggat ttgatttcta tgctccacaa acatttataa
    15961 aggactcaca aataatacta tttgcatggg ctggaatggg agaagtttta taccctacag
    16021 ataaaaacaa atgggcacat tgtctaactg ttccaagaaa attaaatata aaaaataata
    16081 agctattgca aatgccagta gatgaattaa ttaaattaag atatgatgaa acaagtgggc
    16141 aaaatactat aaagaataat attaatatta tagaaaatga tgaaaatgta tatgagctta
    16201 atattaatat aaaaaatatt gacagcaata aatttgggct ggaattattt tcatcacaag
    16261 atgaaggtgt aaaattagaa tttaacaaat taggaaacat tgtaacttta gacagaagca
    16321 actttaaaaa agttttcggt gttgaatatg gaacaaatag aaaagaatat ataaatattg
    16381 atgaaaatac taatatcaaa gtactagcgg atagaagtat tttagaaatt tttataaatg
    16441 aaggagaagt agtatttact agtagaatat ttgctaaaga aaattctaat caaataaggg
    16501 tttatagtga taaaatagta gagtatgagt atactaaatt taaattaaag caaggtattg
    16561 aattataaaa acacattata aggggtagaa aagatgaaaa aagtaattag cataggagaa
    16621 gctttaatag actttatacc gaatcaaaat ggtggtaaat taaaaaatgt atcagaattt
    16681 agaagagtag caggaggagc tccagcaaat gttagtgctg ttgtagcaaa gcttggagga
    16741 aaatcgagtt ttatatctaa gttaggaaaa gatgcatttg gtgattatat aatagatgta
    16801 ttaaatgaag taaatgtaaa tactgattat gtattgagaa caagtaaagc aaatacagga
    16861 cttgcttttg tatcattaaa agaagatggt aatcgtgatt tctcatttta tagaaatcca
    16921 agtgcagata tgcttttaga agctgatgaa gttaaaaaag agtggtttaa taattgtcat
    16981 atcctacatt tttgttcagt agatttaata gatagtccaa tgaaattagc acataaaaaa
    17041 gcaatagaat acgcacttga aagtaatagt ataataagct ttgaccctaa tataagatta
    17101 cctttatggg atagtgagca atcttgtaaa aaggcaatat cagaattttt accttttgct
    17161 catattgtta agatatctga tgaagaatta gaatttataa caggagaaaa tgatatagaa
    17221 aaatcattac ataaactctt tgtggggaat gttgaactag tattatatac aaaaggaaaa
    17281 gatggagtag atgcatacac taaaaaagta aaaggtatgt gtaaaggggt taaagtaaat
    17341 gcaatagata caaccggagc aggagattct tatataggtt catttttata caccttattg
    17401 tataaacaaa taacattgaa tgatataaaa gaaatgaaac aagaaacgtt aaatgagtat
    17461 ttagaatttt ctaattatta tgcagctcgt agtacaacta ttaaaggtgc gataagttca
    17521 tatgcaacaa aggaagaaat aactgaattt ataaaaaatt tatagtagga atgagtaata
    17581 ggttttgaag gaagtaacat gtaaagagtt tttaaggatg tttactatga aaataattaa
    17641 catataaaaa gcaataaaga ggctgtctca aaatagagat aagcctcttt atcattgttg
    17701 aaatgataaa aaatatttta attattgtta ctttttagaa gaatatatat cttattttaa
    17761 ataagcaaat tatgtaagat ttatttatat tatcaatact ttacttggag tattatgtct
    17821 ttagcagtta tctacttcaa ttgaattatc taaaatataa taaatttgtt ttgcaaattg
    17881 acgagaatgt tcttcttttt ctttattcat aaaattaggt ttacctaaat cacgttcaac
    17941 aagcattcct atatatttta actgtgaatg cttacagtag cgcttcatac cattttccca
    18001 aatatcagca cccttttcag gtctatatcc acatgttgta ataagtgcta ctggttttcc
    18061 tgcccataga gtggggcctt tctcattacc atagtattta ttcattccat acaccaatct
    18121 atcaagtagt gctttcattg gagaagtgca gtaccaagaa taaattggag tagcaaaaac
    18181 aattaaatca cacataagaa ttttcttgaa aattttctga acatcatctt tacaataaca
    18241 tccaaaatca aataagttat cctgacattt tttacaagca atacatggct ctattttaca
    18301 atcatataac catattactt caatatttga attataattt tctaattcct caataaaagg
    18361 ttttaaaata gcagcagtat tacctttttt cttaggactt cccataagaa tgcatacttc
    18421 catattgata cctccacagt attttaaaat agtttttagt gcttaatata gttttaggat
    18481 actatgagaa tacgttaaca acaatacact ttgtatattt gtaataaaat tgaaacaaat
    18541 tgttttatca ataaaagttg gaatgtatct ataattaaaa atattttagg tatgttttta
    18601 catatatgtg tgtagttaac tatggtaatt tattgtaaaa ttatcttata ggattataat
    18661 tgcttaattt atgattatat ttaaaatctt ggaggtaaaa aatatgtact caaatgaaga
    18721 ctataaattt atagaagaaa atgcaccatg ttatatatat gatgagacta atataattga
    18781 aaaatgtaag gaattgaaaa ataatatttt aggttttgat tttttatatt ctataaaaac
    18841 aaatccatat aacaaaattg taaaaagcat tgcagagcaa ggttttggag cagatgcttc
    18901 ttcaactaat gaagttatga tttcatcaga atgtggagtt tctaaagatg atatttatta
    18961 ttcatcacca ggcaaaacag agattgatat agaaactgtt ttagataaat gcataattat
    19021 tgcagatagt attacagaaa tagaacttat aaataagata tctaaaaagt ataatcagaa
    19081 agttaaaata ggaataagaa taaatcctga ttttactatg gatgatgata gtgctaaatc
    19141 aagcaaattt ggtatagata tagaagatat gagcagtgtt tttaagatgt taagtatgtg
    19201 tgattatgta tctgtagttg ggattcatat acatattaaa tctcaggtat tggattttga
    19261 aaagttagga cattattatg aaaaatgttt ttttatagct ttaaatttaa ataaaagtaa
    19321 gtatattaat attgaattta taaattttgg tagtggactt ggagtagctt atgataatct
    19381 tagagaaaat ccacttaatc ttaaagagct ttcaaaatta actcaaaaga ttgcttttac
    19441 taattcaaac acactaaaat caagacttct tattgaatca ggacgtttca tagtttgtaa
    19501 atcaggtaat tattatgtta aaattgtaga tattaaagta tcaagaggga aaaaatatct
    19561 tattgtaaaa aatggactta atggatttat gagacctatt atttctaatt taattgaaaa
    19621 tattacttgt ggagtagaat tagaaggtca agagccatta tatacatcaa atcatgcctt
    19681 ttctctttca gtattaaata atttaaaaga agaagagtat gtaaatgttg ttgggaatct
    19741 ctgtacatca ttagatgtaa ttgcaaataa tgttaaattg aaaaaagcaa acataggaga
    19801 tttgatttca attagtaatg ctggaagtta ctcctattct ttatcaccgc tattattttc
    19861 aagttatgaa tttcctaaac aatattataa aagaaaagat ggatatattg agtaaacaat
    19921 agcataaatt aataaactta gtattattga aaaaatttaa aatgcagggg gtgtaattct
    19981 atatggaatt gtggaaaaga aatttatttg tatgttggat aggtatgttt tttagttcta
    20041 tagggatgag ccaaatagca cctatacttt cattatatat taaacagttg ggggtaactg
    20101 atgttagttt aatacaacag tattcaggta tagtttttgg atgtaccttt gtagtagcag
    20161 cattcttttc gccaatttga gggaaagctg ctgataaata tggaagaaaa tcaatgcttt
    20221 tacgtgcaag tattgggatg ggaatagtta tatttaccat ggcgtttgtt caaaatgtct
    20281 atcagttatt aggattaaga atacttcaag gagtttttac gggttatgct actgcatgta
    20341 ctactcttat tgcaacacag acagataaaa atcattcagg atgggctttg ggtactcttg
    20401 caactgcatc aactactggc tcattaattg gtccaacagt aggaggatat atagaatcaa
    20461 tcttaggtct taaaagtaca tttattataa caggaggatt gttgtttgta tcatttatca
    20521 tctctatttt atttgtagta gataatttta aacctaaaga aataaaagaa tcaatttcta
    20581 tacctaaaga acaattaaat atattgccaa ataaattttt aattgcatct atttttgtaa
    20641 ccacattcat tactcaacta gctttatact ctatagaacc aattgtaacc atatatatat
    20701 cacaacttac aaattttgca tcaaatgtag ctttaatagc tggattgaca ttttctgcat
    20761 caggattagc aaatttatta gcagctcaaa aacttggaaa aatgtcggat aaaataggtc
    20821 ctcaaaaagt attgttaatt tcacttttat gggctgggat tatatttata ccacaagctt
    20881 ttgtaaaaac tgcatggcaa ctgatgttgt tgagattttt attagggttg tcagttgcag
    20941 ggcttaatcc ttctgttaac agcttgctta aaaaaattgc accagaagaa tatgtgggaa
    21001 aaatatttgg atataatgca tctgcccagt atataggctg ttcatcagga gcatttttag
    21061 ggggacaaat atcagcacat ttaggtatta gaacagtatt tttttcaaca agtttattgt
    21121 tgtttatgaa tgcactttgg atgtataaat ttactggatt gtttattaaa gataagacta
    21181 aataatcata ctatttacat ttctaatttt atgatgtaaa ataaatattt tttatgtaat
    21241 ttatcataga attatataaa aaataaccct atggtaaaaa ttggaaaata ttattatcga
    21301 caaaatcttt gacttaaaaa ctatattgcg atagtattaa tttaataaaa gtttgagagg
    21361 ggcgtaaggt tatgacaatt tcaaaaatac cacaagcaga attaaaagtt atgaagttta
    21421 tttgggaaaa aaatgataca gtaacatcaa aagaaactat tgaagctatg gaacgaaaat
    21481 atggttggaa acaaacaact acattgacac ttttatcaag acttgttaaa aaacattttt
    21541 taagtgcaga aaaaatagat agatacactc attatacaat agttgttgga cataaggagt
    21601 atttaagtgt tgagacaaaa gattttttaa gcaatataca cgacaattct ttacagagtt
    21661 taatttcagc actacatgat gatgaagttg ttgataaaga tatgttggat ttatttgaaa
    21721 gtcattttaa aaatttagaa gaagagtaat tatatcagga tagaactcta agaatttaat
    21781 taatctatgg ctga
//
LOCUS       DAGSHT010000048        21393 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.049, whole genome shotgun sequence.
ACCESSION   DAGSHT010000048 DAGSHT010000000
VERSION     DAGSHT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.049"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(170..949)
                     /gene="hisJ"
                     /locus_tag="MJB72_001611"
     CDS             complement(170..949)
                     /gene="hisJ"
                     /locus_tag="MJB72_001611"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBY8262728.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1495..1845)
                     /locus_tag="MJB72_001612"
     CDS             complement(1495..1845)
                     /locus_tag="MJB72_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBY8262729.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1861..2277)
                     /locus_tag="MJB72_001613"
     CDS             complement(1861..2277)
                     /locus_tag="MJB72_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBY8262730.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2296..2487)
                     /locus_tag="MJB72_001614"
     CDS             complement(2296..2487)
                     /locus_tag="MJB72_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBY8262731.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2638..3453)
                     /locus_tag="MJB72_001615"
     CDS             complement(2638..3453)
                     /locus_tag="MJB72_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBY8262732.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3500..4438)
                     /locus_tag="MJB72_001616"
     CDS             complement(3500..4438)
                     /locus_tag="MJB72_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8262733.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVNKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4435..5307)
                     /locus_tag="MJB72_001617"
     CDS             complement(4435..5307)
                     /locus_tag="MJB72_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8262734.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5332..7260)
                     /locus_tag="MJB72_001618"
     CDS             complement(5332..7260)
                     /locus_tag="MJB72_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBY8262735.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7372..7749)
                     /gene="gcvH"
                     /locus_tag="MJB72_001619"
     CDS             complement(7372..7749)
                     /gene="gcvH"
                     /locus_tag="MJB72_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBY8262736.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7836..9962)
                     /gene="cdhC"
                     /locus_tag="MJB72_001620"
     CDS             complement(7836..9962)
                     /gene="cdhC"
                     /locus_tag="MJB72_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBY8262737.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10019..10825)
                     /locus_tag="MJB72_001621"
     CDS             complement(10019..10825)
                     /locus_tag="MJB72_001621"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBY8262738.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10875..12242)
                     /locus_tag="MJB72_001622"
     CDS             complement(10875..12242)
                     /locus_tag="MJB72_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBY8262739.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12273..13217)
                     /locus_tag="MJB72_001623"
     CDS             complement(12273..13217)
                     /locus_tag="MJB72_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBY8262740.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13237..14007)
                     /locus_tag="MJB72_001624"
     CDS             complement(13237..14007)
                     /locus_tag="MJB72_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8262741.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14063..15448)
                     /gene="lpdA"
                     /locus_tag="MJB72_001625"
     CDS             complement(14063..15448)
                     /gene="lpdA"
                     /locus_tag="MJB72_001625"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBY8262742.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15492..16373)
                     /locus_tag="MJB72_001626"
     CDS             complement(15492..16373)
                     /locus_tag="MJB72_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBY8262743.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16409..17083)
                     /locus_tag="MJB72_001627"
     CDS             complement(16409..17083)
                     /locus_tag="MJB72_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBY8262744.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17120..17992)
                     /locus_tag="MJB72_001628"
     CDS             complement(17120..17992)
                     /locus_tag="MJB72_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBY8262745.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18084..18716)
                     /locus_tag="MJB72_001629"
     CDS             complement(18084..18716)
                     /locus_tag="MJB72_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBY8262746.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18821..20497)
                     /locus_tag="MJB72_001630"
     CDS             complement(18821..20497)
                     /locus_tag="MJB72_001630"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBY8262747.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20746..>21393)
                     /locus_tag="MJB72_001631"
     CDS             complement(20746..>21393)
                     /locus_tag="MJB72_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HBY8262748.1"
                     /translation="ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVD
                     DIPENFCKEYNGVRLLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAG
                     IEHLGRGTAQGVDAFIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEE
                     FIIQNLEDGESLGFIYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
BASE COUNT         6269 a         4424 c         2422 g         8278 t
ORIGIN      
        1 ttctattgaa ttttattatt attttattta gattctattg ttattttact tagaacttat
       61 tattatttta tttaagttct attactattt tcaatcatat aaatttacaa aagactgtac
      121 ataaatttat tttatacaaa cttatctaca gtcttttgtg tgaatatatc taaataatat
      181 cgtatttatt tatttcctct gcacagtgag aatcagaacc taaaactaat ggaatatcgt
      241 atttttttac caactctaat aagtatccat ctatataagg ctctttgcag tactcttttc
      301 tatttccaga aacattataa tcaagttcat aacctttttc tttaattaac tttattaact
      361 cttcaagaat tgtatttcct ttataatcgt atggaaatac ctgattaaac tttctaacta
      421 aatttaaatg acctattctt tttggtttat gtatacctaa atctgaattt acagctttta
      481 tcaatgtttc ataatactta ttataaacag cttcaatact tcctaacttg tcgattaaat
      541 tttgaaactc ctctacactg taatctacac aataataatc atcatcaatt ttaattatat
      601 gtactgataa aagagaatcc tctaattctt ctccatattt atttaaattc gcctttatcc
      661 cttcttcgta tccttctata aaatccactt ccgacccaac atttatcttg attttaccct
      721 catacttttc tttcaacttc ttgacatcat taaaatactt tggtaaatct tcaatactca
      781 tacaactatc attttcagga gcagggtctc taaatccatt tgcatacgga aaatgttctg
      841 taaatgtcat ttctgttatt ccaacatcca ttgctctttg tacatatttt tcaaaatcat
      901 ccttacttcc atgaggacaa taaggtgaat gtatatgacc atctttcata aataattcac
      961 tcctttttta aagaaaaaat ctaatataaa tgcataaatc ttttaacttc aaaataatat
     1021 ttttaatcat gctaattttt attatatcta atattagagt aattttaaac tgccttttaa
     1081 gtatcttatc attagtacta ttttcaactt aacattaata ttatttttta acttaacatt
     1141 aatactatct ttaaatagta atactcaata tataattact taacataaaa taactcaaat
     1201 tgcttgatga ggcttttgca gtatttaaag aatatataat tatttaacat taaattatcc
     1261 aatcttgtaa taaaatttac tcattaaagt atattacaga aaatacctca aaataaactt
     1321 tttagttcat gagatactct tttttacatg aaattagata tatttttatc atttcaacaa
     1381 tgaaagggag gcttatatcc atctttagac aaccccttag ataaaatttt taataattaa
     1441 gcatcacaaa atataaaaat cgcttatatc aaacgtataa gcgattaatt atatttacat
     1501 atgtttttta gcctctaaca acattttatt agcttcttcc atatactcta tagccttttc
     1561 aatactcttt gctgtttctt cttttccaat agctctagcc ttttcaaccc actctctaaa
     1621 tccttcttca tgagtttcat tgtgatttat ccaatgtact aaaagtattt ttaaagtttt
     1681 ttcatcctta gactctgatg attcctcatg actatgttcg tggtcgtggt cgtgagcatg
     1741 gtcatgtgga tgttcatgac atatatctcc atgagtatgt tcgtggcagt ggtcatggtc
     1801 atgatgtcca tgatgatatc ttacaatctc tttatcaaaa atcattagtt attccccctg
     1861 ttattttata ttaaactctt tttttataag gtttacagaa tcttttacaa gtagtgctaa
     1921 tggtttacct tctaatccaa ctaactccac attttctggc ataataggta aaagtatctt
     1981 aagtgcctca ctgtctgcaa tagcttcact tatattacaa gtaacttctc ccatcataga
     2041 attaggtatt attacagaaa taggtgcaac tattatattt gcttttttag ctgatactac
     2101 tatagcattt tcccctgtag ctcctttatt agcatgagct ttcatcatag aactagttgc
     2161 tatagaatta gtccctagtg cataaatttc tacatatgta ggtaactctt ctctcaaaga
     2221 agaaactatc tgggctccaa tacctccacc cattccatct atcactgcta ttatcatatg
     2281 aaattcccct attaattaat tttcttgtat tattatctta tggtccatca atcttatttc
     2341 tttaattatc ccatctatta tcttttgctc tcctaataaa tccgttaaat aaatctttcc
     2401 atcacaaggg tctatattaa ccacattttc cataaccttt tctaactcat tattactttt
     2461 acaaatgaaa gctgatgatt cacacatata aatctcctcc taatatgatt tttattaaaa
     2521 atcaaaaaaa gattccctta ggtatttcaa tacaccaaag aaaccttcat gatttcagtt
     2581 tttgtatatt attatacctt aagtatatca ctttaattta tgtaaagtca attaaagtta
     2641 acgcttttta ttatactctt cattcttgta tttttcatat agttctttaa ctaattgctt
     2701 ctcataatca gtcaaatcgc cctccacaaa ctcaacatta aattgttcag ctaatccctt
     2761 tactatgttt ttctgtagaa tatctatgtc tatttttctg ccaatttcat tttctattcc
     2821 acttgctttt ttagctgtaa atttcatagc tctttctttc aattctggtg tcttagtttt
     2881 tattaattta aacaattttt ctacatcaaa atccagtatt attgaaccat gttgtaataa
     2941 aacaccatct tttctgcttt gagcacttcc aattaccttc ttattattaa tagtaacttc
     3001 atatgatgca tgtgcattaa aacatgcagc tgataaattt tccctactta ttctctcgcc
     3061 tctatttaaa ttatctgttt ctataccact tagattcaac cctttaacta gaccttcact
     3121 tatatatcta taagataggt taatgctctt atcgattaaa ggattatctt ctcctataat
     3181 tatactatag gttagctcat tatcatgtaa tacagctctt ccacctgtaa ttcttctagt
     3241 atagtctata ttcatacatc tacacttatc taaatctatc tcatcttcca atttctgaaa
     3301 atatcctata ctcaaacaag ctggttccca tgtataaaat ctaagagttg gcttattatg
     3361 tcctttctta tatgcagtaa atatagcttc gtcaattgcc atattcattg ctccttcata
     3421 tgatttatta tgtataactc tccactgatt catgattatt acgcccccat ttttagtata
     3481 aatatatcct agaaatcctc tatagattta tttctcttat tgctaaatct atctcacttt
     3541 gatttaaagg tcttgaatag ttgtatatag tacttcctgg tctttcatta tagtatggtc
     3601 tattacaatc cttacaacct cttatttcaa aaggatagcc tttgtttatg tcttttaaaa
     3661 tatcattgtc aagctttata ctatttaagt atccattttt aaacttaaaa tactcttttg
     3721 gataaccttt atttatcata taagacatta actgtaccct tctataactt tctatgcttg
     3781 gttgacttat cttttccatt ttagttcctc taactggagt aaatgcaaat aaactaatcg
     3841 ttacatcatt ttcctttaaa taagtatata aattgtatat atcttcatga ctttcaccca
     3901 taccaactat tatatgtgta cttattttat tgggatatga ttttgacatt tcagttataa
     3961 attttaactt ttcatcatag ttatttcctt ttattttttc atatagttct ttatttgctg
     4021 catctattgc tattcctatt ttatttactc ctgctgaaaa aaacttcttt atctgttcat
     4081 cattttctaa ttttgcagat agtgaaattg gcatgtctat cttaccactt atatagttta
     4141 taaagtctaa aacagagtta tgtgcttctt cacttgccat tgattgaata catattctct
     4201 ttatattttt tcctttataa gcttttaaag catctaatat ttcttctttt gaaaactctg
     4261 gccaaattac tcttgataat ttatcttttc tagtactact ttctattgat tgagaacaaa
     4321 aactgcattt atttatgcat ttttctccaa tcattatata tgctgtggta ggaggtatat
     4381 cgcttttttt gtttaatatc ccaagttcta ttgctgtccc aactgatagt ctaatcataa
     4441 aacgcaacac tccttacact catttattgt catattcatt tctatagcct tattctttgc
     4501 agaccttgat ggtaatacta ttctattgac tccacacatt atagataatt ggtctagctc
     4561 ttttctatac actcctcttg gtctcataca acctagatta atttcagtat ctggtaaatt
     4621 aattctagct tcacataaaa tatctgctac cccttctaac tcaggaggtt ctacattttc
     4681 atactcagtc ccttttgttg gaataaggac tataaatgta agcttattag gaggattttt
     4741 ttgtaggtat tctataattt catattcacc ctttatttgt cctcctttta aaccaataca
     4801 tatatgtggt gctacttctg tatgttcttg tattgtatta tagacttcaa tataatcttc
     4861 tttactcttt ttcatcttat aaacttctct tattgtctca tcatcaaaca ctaaatcaaa
     4921 agaaactata tctaaatact tacacaactc tacaatgcta tctttatcca taagacctaa
     4981 atgagcattc aatctatatc cttgctcttt taagttttta atggtattaa tatgagtatt
     5041 tatagggaca tctccctcat aactacaacc gccactcaat agaaagctag ttatattcct
     5101 tgattgtact ttttcctcat attcttctat tggcaccata ttgtttaagt agtgaccgtt
     5161 gcaatgggca catttcaaac tacattgatt gcttgtagta ctaagagcta gtgtctggtt
     5221 aggataagca aactctattt tatttccaaa attctttaat ttaatatcaa atgctatttt
     5281 cattttttca gatagattct tattcatagt ttattccttc ttaagtacat tttactcttc
     5341 tagtaatttc tttgctgttg ggaactgctc tatattcgta tgtggtaaga aacatgcaga
     5401 aataaattca tccatataac ttggatatac actaagttca acataagtta tttgattacc
     5461 tatttcttca agtttatttt ttgcatcttt acttataaga gctaagtaag aaccaacaag
     5521 tgaactattc cctatataag taaatttttc tctatcgata tcaggaagta accctataat
     5581 tattgagttt tctatgtcta ggttgtttcc tataccacct gctatataaa ccctatctaa
     5641 tatactaaaa tccataccta aactatctac aagagtgtat gcgcctgaat atatagctcc
     5701 tttagctctg atgaaattat ctatatcaac ttcattgact actatatcat tttctaaatc
     5761 aaattcttct ttaaatgcta atacatactc tcctatctca tgctcattaa atctaactct
     5821 cttattatct aaatccctgt atattttacc tcttctatct attacacctt tagtaatcat
     5881 ttgacagatt aaatcaatta taccagaacc acatatacca actggtgcac attcatcaat
     5941 aatctttaat gttggctcta aagtatcttt atcaataatt actttctcta ttgctcctgc
     6001 tgatgctctc attccacagc ttatccctcc accttcaaaa gcaggtcctg ctgaacatgc
     6061 acagctcatc atataatctt tatttccaaa tacaatctct ccattagttc ctaaatctat
     6121 aaataaaaca ttttcttcac ttgaccatat ccctgctgat aatactcctg ctgttatatc
     6181 tcctcctaca taacttgcaa cagatggagc taagtacaca tatgcactgt catttacaaa
     6241 aagaccaaca ttttcaccca taagttttgg agattttaaa aatggtggta tgtaaggttc
     6301 ttgtcttaag aagtctgtat acacacccaa gaataaactt gtcatagttg tattcccagc
     6361 agccactaat gtaactacat cttccttatc tattccattt ctttcatata ttgtttttaa
     6421 tagtggattt atagtttcct caactatagc tttatgaagt gtctctaatc catttttctt
     6481 agttgaatat ataattctat ttataacatc tgctccatat tttatctgtg catttccaga
     6541 agatgcttta tctactacct cttttgagta taggtctact aaacaaacaa ctactgaagt
     6601 tgtaccaata tctattgcaa ctccgtataa ggaattttct ttatttcctt gttctatatt
     6661 tatgattgta agtttctttt gtttttgaac atatgttatt gtaaccttaa aatcactctt
     6721 tctaaataca gttggcattt ttctaagtat atccaatcta aaatctattt cattatatcc
     6781 taggttattt ctaacatatc tttccaatct atctacatca cttatattat catctaaatt
     6841 tggttcttcc atttctatgt atttcttttt tatatttgtt ttaaattgaa ggttatgttc
     6901 ttcaattatt tttttagctc tttcaaaaat ttccctatcc tcttttttat tactaccttc
     6961 tatcttcata ccatgcatag aggatgatac tttagaagga acttcaattt ctatatctga
     7021 tattaccttt gtacaacaag ctagtatata accttgctcc cactcatcat ctgttatatg
     7081 tcttgttttt tctgtatcaa cttttccatt caataatttt actttacact tcccacaaga
     7141 cacgtttcca ttacaaggag catctatgaa tatatctgca tttcttgcaa cctctagtaa
     7201 tatatccccc tcattacaat aaacctcttt attgtttggt gtaaaactta cttttatcat
     7261 gttatccctc ccaatttgtt ctatatatga taaaaggaaa gtatagctaa atttgcgtgc
     7321 actcaaattt tactatactt tccccaatta taataatatc ttaatcttta attattctaa
     7381 tccagcctcg tattctttat cacttaataa cgtatcaagt tcagccacat cagctagttt
     7441 tacttttaat atccagtttt catatgggtc ttcatttata cattctggct catcttctaa
     7501 tttttcatta acttccaata cctcaccatt aacaggagat attaaatcag aagctacttt
     7561 agaagactca actactccaa aatctactcc cttagttaca gtatcttcta cttctggtgt
     7621 ttcaacaaac aatatttctc ctaattgatc ttgagcatag tctgttatac ctatatatac
     7681 aacatctcca tcaattactt ttacccactc atgatcttta gagtatttta attctggtaa
     7741 taatttcatt tcgatagacc cccaatatta tattattgat taaattactt aaatcatatt
     7801 aatattatat catattattt taaattttcc atcaattaca ttacactttc catagctaat
     7861 gctggatgac ctttttcttc taagaaagct aatactcctt cagaatcgtc agctatagtt
     7921 tcatcacaaa ccatatcaca gaagttttct attccagtca tttctttaac tgtagcattt
     7981 aatttatcag ctacataatc ttttaattct tttggcatcc aaactattct ttctggtcca
     8041 ccttctgcat aagcgaattt tttagaagat atgaaatgtc ttccgtgtcc cataaatcct
     8101 ggagtttgaa ctcctccacc tgtcatagaa gcaagttctc cgaaagtcat accaactgga
     8161 gtaacacttg caaattctct gttaactaca acaaatccat ttgcttctgg cattatacca
     8221 cagatacatt cgaagcatcc acaagaagtc attgggtctt ctagtataga gtataatgta
     8281 acagattcaa ctgcaccttg agatatttgg tttacagttt catttactga attccataca
     8341 cctgttctat catctaagca ctcgcctttt tctattggtt gacaaggacc tgttgggtct
     8401 aattctttag tagccttagc atctaaccag ctaactgctc cacaaagacc aagtctttca
     8461 ggagtaacaa cacaaacgtg agctggtgca aatgattgac ataagttaca agaatagaaa
     8521 gtgtcaacac tttcatcaac taatgaagct aatctttcat ctctagcatt gtacttagct
     8581 attgcttcac cttttaattc agaaactttt tcagcatctg taattatagt tatttcacac
     8641 ttatcaacta ctgattcaaa ttcatctaac atcttagcat ataaaacttc tcctatatgc
     8701 tcaagtctaa atcctttatc aaatgcatct ttagagattc ttacccaagc catatctctt
     8761 tgtccaacgt gcattacacc ttctatatag tttaagaagt agtggaaacg tctttcaaga
     8821 actggctcga aatcttcttg catattttta ccagctattt taactattac agcaagtggt
     8881 aatcttaata caccatctgc atcaacttca tctatatttg gtcctattat ttctatctta
     8941 tggtcttcaa cttctgttgc ttctttctta acaacaagct cccaagcttc agttctgttt
     9001 ccaccaaact cagcaaacat atcgctcttt ctaattctct caccttcaaa tgctgctgca
     9061 aatgaaactg ggataggtat ttcagtaact tttattttta ttcctctagc ttctaatgaa
     9121 gtagctacta tcttatcgta atctttttga gttaataggt tcattggaac ttcaagaact
     9181 gtttggtcag ttataactgg gaatcctaaa gctattgctc cagcacctgc agatacaact
     9241 aattcactta atggtccaaa tgcattaaca aatgcaggaa ctctattagc tgtgtactct
     9301 aataatccgt ttaaatcacc aggagttaat ccaccgaata ttaatgctgc tcttactgca
     9361 acagatacaa cgtgtataac agaagttaca tcatatccta gaggtataac tcttagctca
     9421 agacccattt ttactccagc ttctatagct tggtctataa ctttaccaac taagaaagtt
     9481 agtaaacctt tagattggta atcttttata acttttgctg ctgattctgc atctggacac
     9541 tcaccaagaa caactgcaac accaggtata tctcctgtaa ctaatggtac accaagtgat
     9601 ctgattatag ggtcagttat atgacctgca catggctcac tatatggagc atccatagtt
     9661 gaatatttta atgcttcaat tacttctgct gctaaagctg tagctaaacc agcattaaaa
     9721 gcatgttcta gtaaatgagt tctatttatt aaagatttta ctacttctaa agcaccttct
     9781 aagtccccta aagtgttcat cttttgtcct gttgcagcat atatacaagg taatgaatat
     9841 gctgtatcag ggaaagcaac tttatgctct tttccattct tttctatagc ttcagctaac
     9901 atagcttgag ccgcacctaa agcttgttct gaccctgtaa agattatatt atatagattc
     9961 attctatttc cccctttaat atttacacct attttttatt aactataccc taaaaaccct
    10021 agaaagcaaa tccaccttca tttaactcta agaaagagtc tgcatataaa gatgccccgt
    10081 tgattatgtc acaagatttt acagttttca ttaattctgg gtcacatgga ttcattatag
    10141 ctgaactaaa tccattagcc attgccattg ctaagaatgc actatctaaa acaggtctaa
    10201 catgcttagg acatccatta gatacgttag ataatcctcc tgtagttaaa agtcccatct
    10261 cagtcatcat ttttatagct tctaaaactt ctacttgttt ttcttgcata ccttttataa
    10321 ctaagcataa tgggtcaaat agtatatcag ttggctccat tcctaacatt aagccttttt
    10381 ctaatatttc ttggcagtaa gccatacgct catcattatc tcttgggata ccttcttttg
    10441 cacataaacc tataaccatt gcttcgtatt cagctgctat atctattaaa tcaaatcttg
    10501 agccagcatc agcagagttt attattggtt ttgcattagt tctatcataa actttaagtc
    10561 cagcttcgat agctttttta ttagctgtat ctaatgctat aggaacattg ttgaactcag
    10621 attgaagtaa tttgattccc catgtcatta tttcttcgcc atctctttcg gcaggtccta
    10681 tattaaaatc tatatagtgt gctcctgctt ctaattgctc ttttgctctt tttaagattg
    10741 gagctgggtc tctttctgct aatgcttttc ttattgaagg tgatatacaa tgtattctct
    10801 cacctataat cataaatttt tccatttata atgtccccct ttaaatttaa tattaatagt
    10861 tagttaatat tttattagtt tgtttggcat gctttttctt ggtatccttt taagaactta
    10921 ggtaattcca tagattcctc tggtcctata actacattcc atcctggtaa gttatcttct
    10981 atatcacctt taagtactgc aacttttcct ggaatgataa ggtctttaca attagttact
    11041 tcttctacct tactttcttt aatgaaagca gagattgagt ttccaccaaa tttaccagca
    11101 gcccaagatg taagaactga atatccacct gcatctggta ttactaacca tactggaact
    11161 tttgatcttt ctatatcacc agcaactatg aagtaagtta atgcaaagtc aacagtaact
    11221 aatactggag aattttcatc tgggttgttg attggataaa tctttggttc aactctcatt
    11281 ggtctttgtg ggtcagtgta tatgttttgt cttagtgcaa ataatggtaa tgccttagca
    11341 taactaatat catctattac tattatagaa ccgtatttta tagtgaatat tgatgataaa
    11401 gcaacttcca tcatagggtt tgaattagat aatctatttg caaatactat tgaaggatat
    11461 ccaaatgttc tatcttgctc ttttaaagct gttcttctaa cttgtatagc attagtgtaa
    11521 gtatctttta tgttttctcc tgttacatct aatactaatt ctttatatcc agcagcttga
    11581 attaattcaa ctgtttcata taattcttct aagctaccag cttttacacc taatggtaaa
    11641 cttgctcctt taactacttc tatcatatct ttgtagttct ctttagttgc tccatatacc
    11701 attggtctag catcttttaa tacttcaaca gcttctttaa ctacttgtgc atcatcacaa
    11761 gctaaaatat aagctacttc taatccactg ccttttattt tatctattaa ttttaaatac
    11821 gcatctttat ctccacaata ctctaaaaca gccatttcaa ctttcatttg ttcaccaatt
    11881 cttacatagt caactttttt catgttagct attttagcat ctactgcttc atcactcata
    11941 caagtacaga atgaaactgc atatctattt ctacttacta atgttttttc atgtctaaat
    12001 aatactgttt ctccacctag ttcatactca gatgcaccag caccaacttt taaagctttc
    12061 attaaaggtg cagtagcctc tgataatttt gctatagcgt catcagacat atgtgggcat
    12121 ttacctactt ctacagctcc tgaagcaacc ttcatagaga aagccataca agttgggaat
    12181 ccacaatcct tacagttttt ctttggtgtt aatttaaata tatctaaagc ttttaatgcc
    12241 attatttttt ccccctaacc ttaacaattt aattaagcta atgcgttaac caattctttt
    12301 atagtttcta ttgattttgg atgacgaagt ataactgcgt ttgaaccacc tactaaaaca
    12361 cttgctgcag ttgaaacttc cattgctata gttctttctt ctggacaacc ccaatctggc
    12421 tcatcttcta ttggagctat agcttcttta acatgaccta cttcaaaagc tactggtgtt
    12481 atgataggca tttgtaatgt cttatcattt tgaccaaatg ctgcaagtct aattctatcc
    12541 atagtagatg caacgtactc atatccataa ccaactgctg aacatccaac attcataact
    12601 atattttcac ctttaacgcc tagttgagtt aacaatacgt ttaattgttt agctaggttt
    12661 atatcaacag aagattcagc tcctacttta tgtgcataag ccataccagc tgatgctcct
    12721 actcctttgt agttatcttc tactgctgac ataaataagc agttatgtcc atccaatgtt
    12781 tgagctaatt tttcaaataa tttaccatct ttttcatgat ttcctgaacc agctacaact
    12841 aaaggtaatt ttattgcttc aattactgcc ttagctacat cagcacattc atcaactgac
    12901 ttatctaatc catttgggtc agaaccatca aattttaaac aaataaaatc tgcttgtgta
    12961 ttagcttcaa catatttagc ccactctact ggacaattag ctacatcttt gtataattcc
    13021 ttatatgaat cagtccagct ttcaggataa acgtctgata tttgtatacc tatttttgga
    13081 gaattaccta cttctccatc aaaactataa aaaggtaata cattttcccc acctaattta
    13141 attgcttttt cccctattcc tacttcaact tctgatattt ttccagaata tttttgagta
    13201 gacattttaa atgccatatt tttttccccc ttaagcctat tttaattcta atttagcaaa
    13261 aatttctttc atagccactt tagattttga atcttcaggt aaattaacta aaggaatacc
    13321 tgtagaatca tattcatata tcaattcatc cataggtaca acaccgatta gctctaggtt
    13381 atgcttttca atttcttctt taacaccatc attcataacc ccatttggaa ctttatttac
    13441 aatcaaaagt attcttccaa cacttaactt tagttcctca gctaaatctc ttattctagc
    13501 aacagcttgt atgctacgtc ttgagcaatc actaaccaat aaaagtgtat caacatgtct
    13561 agtgactttt ctacttagat gttccatacc agcttcgttg tccatgacta agtatttgta
    13621 atgaccagat attttgttta cttgttctct aagtatccca tttacaaaac agtaacatcc
    13681 ttcaccttct gacctaccca ttactaataa gtcatatcct tcgccttctt cgattataga
    13741 atttaatcta aattgaagat attgtgcctt tgtcatacca ccagggaatg cattgcctaa
    13801 cttttctctt tgatttactt cttctcttat ttctcctatt gttgcttcaa cttcgatacc
    13861 taatacttca tttatgttag cattggcatc agcatctaca actaatactg gtcccttttt
    13921 gtccttaacc aaataatcaa tcaaaagccc tgtgagactt gttttaccag ttccaccttt
    13981 tcctgcaaca gctatattgt atcccatttt aagacttccc ccttaaatct agccacagag
    14041 ccttatgtac tctgtctgtc tattataatt taggtacaga atgaacacat tctccatgta
    14101 catcatgaag tgcttccatt agcccttcag ataaacttgg atgtggatgt attgcatccc
    14161 ctacttgttc tactgttaat cctagatgaa cagcaagtga caactctgtc aataaatctg
    14221 tagcatgagg tcctactact gctgcaccta ttatcttatc agtttctttg tcagctataa
    14281 cttttacaaa tccttggaaa tgtcctatag cttgtgcttt tccaagacct ctaaaatcaa
    14341 attgacctac attgtattct acaccttcag cttcaagttg cttttcagtc ttaccaacac
    14401 cagcaacttc aggttcagta taaacacatc ttggaattgc tctataatct actaccttag
    14461 tcttacctaa agcattttca actgctacga taccttcttt agaagcaaca tgtgctagga
    14521 aaggagtatc tattatatcg cctattgcgt atataccttc tacattagtt tcaagatgtt
    14581 cattaactac tacttttcct ctttccattt caattcctat atcttctact ccagaattgt
    14641 caaggttagg tcttctacca acacatacta atgcatattg tgcttctata acctttccat
    14701 tagaaagagt tgcaacagcc ttaccatcaa ctacttcaca agtttgtact ccaatacctg
    14761 taataacctt aattttatct ttcttaaatt gtctaagtag ttgtttagct acatctttgt
    14821 cttcatttaa tagtatttgg tcaaccatct caactatagt tacttctgtt cctaaggctc
    14881 taaagaactg ccctatctca catcctataa ctccaccacc tactattaac atagactcag
    14941 gtatctcttc aagtccaaga acttcatcac tagttattac aacttttcca tcatatggga
    15001 acattcttgg tacaactgga acagaaccat ttgctagtat tatcttatct gcttttatag
    15061 tttctactgt tccatcatct ttagttactt ctatagtgtt tttatcaatt aattttccaa
    15121 aaccattaac taggtttact cctctttttt caaataagaa ctctatacca cttattaatt
    15181 ggtttacaac tttatttttt ctttccatta tggcagtgaa gtttggctta actgtaccat
    15241 ctatttctat accaaaatcc tttgcttctt ttacagtatt caatactcct gaagaagcaa
    15301 gaagtgcttt agttggtata catcctgcat ttaagcaagt tcctccaact cttctttttt
    15361 caacaactgt tacatctgca ccaagcatag aagcttttat agctgctaca tatcctcctg
    15421 gtccaccacc tactactact atcttcatct ttaattcccc caaacctaat ataatctaca
    15481 atcttgcaat actataaacc tgcggcatcc agtatagcag gtatattctc ttctgcccca
    15541 attgccatta tatgaacacc atcacataaa tcttcttctt ttaattgttt gataagttcc
    15601 cctgccattt ttattccttc acttacccag ttttcttttc cagctgctct taatctttcg
    15661 atttgttcat ctggaacaaa tattcctggt acatttgcag tcataaattt agccattcct
    15721 ggtgatttta aaggtactat tcctgctaat accttgcaat ccatatccct tgttaattct
    15781 ctaaactttc tcattgtatt tatgtcataa agtgcttgtg tttggaaaaa tttagcccct
    15841 gcttttattt tctttttcat ttttaataat tgaacttcta ttggagaata ttctggagtt
    15901 actgatgctc ctagataaaa atctggagaa ccttttaact tattaccagc catgtccgta
    15961 cctgccatta aagtctcagc agtttgaagt atccccacag aatctaaatc aaacactccc
    16021 ttagcttgtg gatggtctcc tacgctagta tggtctcctg ttagtgccaa cagattgttt
    16081 atcccaaata cacctgctga caacatttct ccttgtatag ctattctatt tctatctctc
    16141 cctgttattt gaataactgg ctctaatcca gcatctttta ataatttaca tgtagcaaga
    16201 gaagttgctc tcataactgc cgattgaaag tctgttacat ttgctgcatg aactcttcca
    16261 actaacggct ttgcacattc tattaaatgt gaaaggtcag tcccttttgg aggagccatt
    16321 tcagtagtaa ctgcaaattt tccactttct aatgtttctc ttaataagct catctctaat
    16381 ccccctttta accttataaa aaaaactttt atgaatttgc tgctgcttct ttctttttct
    16441 ttgcacttaa acttcttgga ttattttgct tagaatagtc ttttggtggt cttatttctg
    16501 ctaagttatc taattgtcct atagcttcta gtctttcata tatctttatc caagcacaat
    16561 cattctctga gtttacttca cacttaccat ttttagctcc accacaagcc ccattcataa
    16621 gacctttagc acacatagtt actggacata tcccacctgt ccaaccaagt tcacagtctc
    16681 cacaagcctt acaggcttct tcatattctc caactctttg tacttcccct ataaacaaag
    16741 tattgttggc tggataaact ggcttatctt ttaaattctt aactattgtc tgtgtaccat
    16801 ctccacatgc taaagataaa actgcatcag cttccttagt ctcttctttt agagccttta
    16861 aatccttttt agactttaaa aggttacaag cagggtcaag cattacatat cctaagattt
    16921 ttttgccttc accttcaaga gtttccttca tttttaaaac ttcttcttcc ccaccacttt
    16981 tacaagttgc tgcacattga ttacaaccaa ctaaaaccag cttgtcaaaa tcttttaagt
    17041 accctagcac ttcttctaat ggtttatttt cagaaattat catatctact tcccccctaa
    17101 accttccaca tatatatatc tatttattgt tttgtaattt acaagcctta actacatgtt
    17161 ttgcaagtat agatgttgta actgaaccaa cccccgctgg aactggagtt atcattgata
    17221 ctttgtctaa aactgcattt gtatcaacat ctccacataa atttccttct tcatcaacat
    17281 ttataccaac gtctataact accgctccat ctttaacaaa actttcatca accatcttag
    17341 ctctaccaat agctgctatt agcacgtctg cctctgctgc tacacctgat agatttttag
    17401 tttttgaatg acatattgta acagttgcat gttcacttaa taaaagcatt gatactggct
    17461 ttccaactac cattgatctt ccaagaactg ttaccttact tccttttaaa tcaacattat
    17521 agtgttttaa tatctcaact actgctgtag gtgtacatgg tggaaatcca cttttatccc
    17581 cttccattac cttagcagag tttattggac taaaacaatc tacgtctttt tccggtgcaa
    17641 taacatattt tataacttct tcatttaagt gttttggaag aggtctaaaa cataatatac
    17701 cattaacatt tttgtcatca ttaactctct ttaatacatc aatatattcc tcttgtgtta
    17761 tatcctcagc taactctaac acttctgttg ttatacctat gttttgacat ctctttagtg
    17821 ctcctctttc ataagataaa tcatcacttc ttgccccaac tctaactatt gttagtttag
    17881 gatttatacc ttctttaatt agtaaatcga cttcttttat taattcttca ctaattttat
    17941 cagctacagg ttttcctttt ataatttgtc ctttagttga cattccttcc atacattcac
    18001 aactccccta caagcaaact taaattaatt tattctatat accacatatt aataacatag
    18061 tatctacttt cgtcatttaa aaattatttt ccaagagctt tttcaacttt ggcataaact
    18121 tcatcacaaa tttttacacc atcttcaacc aacttatcta cttctgtttt taccttatta
    18181 acatattctt tatcttgtat agaatttata tttataacta catttaattg tccaccaagt
    18241 atagcagctc ttaaacactg aactcctaca ccaacatcac ttatagcaag tcttgaacct
    18301 ttatcaacta aatcttcatg taacttaata gcttcatagc aaactcttac tatgtttaat
    18361 ggtacttcac aagcaacttt taatgccttt tccattgttt ctgcttttat tcttttttct
    18421 tcttcggttt ccttaggaag cccgtaagct tttgataatg gtaagaaaca ttctgcatca
    18481 tcatctatca tttttaataa atctttttct aactttcctg ccttacttaa tatctctttt
    18541 acactttctt catactcagc atacttcttc ttccctattg taaggttaca taccatacta
    18601 cctaaagcca tacctattgc acctactagt gcagcagctc ccccaccacc tggtactgct
    18661 tctttggaag atagtacttc tacaaaatca acacaagttt tatctgctat tttcatgata
    18721 atcctccttg aaaggatgta ggaaaatatc tcaaattgtt cgagatattt tacgaactat
    18781 tttaagatat ctcctactct ttatttataa attaatttcc ttagaataat ccagctatta
    18841 ctccattttc atcaacatct attttttctg ctgctggaac ttttggtaat cctggcatct
    18901 tcattatttc tccagtaagt gcaactataa atccagctcc tgctgaaatt gttgcttgtc
    18961 ttacagttat tctaaatcct gttggtctac ctaatttagt ttggtcatca gttaaagaat
    19021 attgagtctt agccatacat actggaactt tagtaaatcc aagtttttct aagttagcta
    19081 tttccttatc agcttcagga gtataatcta caccatctgc tccataaatc ttagtagcta
    19141 tagcatttat cttgtctttt atagataaat catcttcata gcagaatcta aagttatttt
    19201 cttcttcatc caataatctt aaaacttctt tagctactgc aattccacct tctccacctt
    19261 ttgcccatac ttcagaaaga gcaacattaa cacctaattc cttacacttg tcttcaacaa
    19321 gtttaagttc agcttcagta tcaagtggga atctgttgat tgcaacaact gctggtaatc
    19381 catatacttg agttatgttt tcaacatgtt ttaataagtt tggaagtcct ttttcaagag
    19441 cttctaggtt ttcgttattt aagtctgctt tagcaactcc accattgtat tttaatgctc
    19501 ttacagtagc aactattata acagcatctg gttttaagtt tgccattcta cattttatat
    19561 ctaagaattt ttctgctcca aggtcagcac cgaaaccagc ctcagttatt acataatctg
    19621 caaagtgcat agccattctt gtagctataa cagagttaca tccatgagct atatttgcga
    19681 atggtccacc gtgtacaaat gatggtgttc cttcaagagt ttgaactagg tttggtttta
    19741 aagcatcttt taataatgca gccatagcac cattagcttt taattgtcta gctgttacag
    19801 gttcaccttc atagctgtat ccaactatta tgtttcctaa tctttctttt aaatcagata
    19861 tatcacttgc caaacagaac gctgccatta tttcagaagc aacagttata tcgaatccat
    19921 cttgtctaac tgctccatca ccttttttac ccataccatc aacgatattt ctaagttgtc
    19981 tatcgttcat gtctacacat cttctccaag ttattttctt agggtctatt ctaagtgcat
    20041 ttccttggtg aatatggtta tcaagcatag cagctaaaag attgttagca gctcctatag
    20101 cgtgaaaatc tcctgtgaag tgtaagttta tatcttccat aggaacaact tgtgcatatc
    20161 ctccaccagc tgctccacct ttaacaccaa atactggtcc tagagatggt tctcttaaag
    20221 caactaatac atttttgtct agtttagcaa atgcatcagc aacacctata gtagtagtag
    20281 tttttccttc acctgctgga gttgggttta tagctgtagt tagtattaac ttagcttttt
    20341 taccagtctc tctttttagt agattgtagt caacttttgc tttgtattta ccataaagtt
    20401 ctacatcatc ttcacctagt cctaattttt ttgcaacctc tctaatatct tgaggcttag
    20461 cttcttgagc tatttcaatg tcagatttaa atcccataac tgaatctcct cccacaacct
    20521 aaatagattt aaaatataat ttttgtaaaa attgtattaa tatgtaaaaa ttatatttta
    20581 taagataatt atacatgtaa aattacttaa aaactagcta ctaagctatt aaaatattca
    20641 aacttaatag ctagttattt atctaattac ttacttatta cctatctatt gcctatgtct
    20701 aacctttaac caacttaaaa tcccaaatca aaatttctat ctattttatt tatccttaag
    20761 tgacattaat ttatctttta ttgctttaat ctgttctttc gttgtttcac tagaatcata
    20821 tggagattga ttggctctat cagaatcaat aacatcttgg ttataataaa taaatcctaa
    20881 agattctcca tcttctaagt tttggattat aaattcttca tcttccttat ttctaatttt
    20941 atttcctact acaaatactt tatttacacc tatatcatgt ccaagtttct taacttttct
    21001 ataagtctgt agacttcttt ctcctggttc tacaactact ataaaagcat ctacaccttg
    21061 ggctgttcct cttcctaagt gttcaattcc cgcttccata tccataacca ctacatcttt
    21121 attttgtagt attaaatgtg agcagagtct cttcaaaaga acatgctctg gacatacaca
    21181 tccagtacca ccagaatcta cggttcccaa tgtaagtaat cttactccat tgtattcttt
    21241 acagaaattt tcagggatat cgtctacttt aggattcatc ttaaacattt ttccaaatga
    21301 acctacagaa gcagctgttc tatctgaaac taattttttc atttctgata taggtactat
    21361 tgattcatat acttcttttg gaaaacctag tgc
//
LOCUS       DAGSHT010000049        21385 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGSHT010000049 DAGSHT010000000
VERSION     DAGSHT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.050"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            141..1334
                     /gene="nifV"
                     /locus_tag="MJB72_001632"
     CDS             141..1334
                     /gene="nifV"
                     /locus_tag="MJB72_001632"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBY8262749.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1359..3284
                     /locus_tag="MJB72_001633"
     CDS             1359..3284
                     /locus_tag="MJB72_001633"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBY8262750.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3306..4301
                     /locus_tag="MJB72_001634"
     CDS             3306..4301
                     /locus_tag="MJB72_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBY8262751.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4627..5100
                     /locus_tag="MJB72_001635"
     CDS             4627..5100
                     /locus_tag="MJB72_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8262752.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5134..5628
                     /locus_tag="MJB72_001636"
     CDS             5134..5628
                     /locus_tag="MJB72_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8262753.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5725..6246
                     /locus_tag="MJB72_001637"
     CDS             5725..6246
                     /locus_tag="MJB72_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBY8262754.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            6435..6746
                     /locus_tag="MJB72_001638"
     CDS             6435..6746
                     /locus_tag="MJB72_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY8262755.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6774..7460
                     /locus_tag="MJB72_001639"
     CDS             6774..7460
                     /locus_tag="MJB72_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY8262756.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7692..8576
                     /locus_tag="MJB72_001640"
     CDS             7692..8576
                     /locus_tag="MJB72_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBY8262757.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNNFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPYIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8675..9850
                     /locus_tag="MJB72_001641"
     CDS             8675..9850
                     /locus_tag="MJB72_001641"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBY8262758.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            9978..10832
                     /locus_tag="MJB72_001642"
     CDS             9978..10832
                     /locus_tag="MJB72_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262759.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            10876..12111
                     /locus_tag="MJB72_001643"
     CDS             10876..12111
                     /locus_tag="MJB72_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBY8262760.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12212..13153
                     /locus_tag="MJB72_001644"
     CDS             12212..13153
                     /locus_tag="MJB72_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBY8262761.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13204..13563)
                     /locus_tag="MJB72_001645"
     CDS             complement(13204..13563)
                     /locus_tag="MJB72_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262762.1"
                     /translation="MDLFEVNIVLNSGTAGGMNPKLKIFNMVISTEVCHHDVTQDTLT
                     EFHSWLKTVFFEANCELIKISEMAVKKTNQEETVFWGRILAGEAFITNKGRKKINEKE
                     AYLHLIASSHIFYTIIK"
     gene            complement(13589..14053)
                     /locus_tag="MJB72_001646"
     CDS             complement(13589..14053)
                     /locus_tag="MJB72_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262763.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDIDMEEIQEAINKTSEETEEKINWTNAWGKKYPILLKY
                     QTDVNISNYAYKVNMMLDELKQEYQYNELEAMLILKDILYNVWKNRKSKRKQ"
     gene            complement(14221..14429)
                     /locus_tag="MJB72_001647"
                     /pseudo
     CDS             complement(14221..14429)
                     /locus_tag="MJB72_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            complement(14473..14826)
                     /locus_tag="MJB72_001648"
     CDS             complement(14473..14826)
                     /locus_tag="MJB72_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY8262764.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            15122..16033
                     /locus_tag="MJB72_001649"
     CDS             15122..16033
                     /locus_tag="MJB72_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBY8262765.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEILEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            16294..16728
                     /locus_tag="MJB72_001650"
     CDS             16294..16728
                     /locus_tag="MJB72_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262766.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGDFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            16751..18286
                     /locus_tag="MJB72_001651"
     CDS             16751..18286
                     /locus_tag="MJB72_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262767.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            18370..19737
                     /locus_tag="MJB72_001652"
     CDS             18370..19737
                     /locus_tag="MJB72_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8262768.1"
                     /translation="MKEYIINNVEEIREKLIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            19772..19996
                     /locus_tag="MJB72_001653"
     CDS             19772..19996
                     /locus_tag="MJB72_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBY8262769.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(20444..20842)
                     /locus_tag="MJB72_001654"
     CDS             complement(20444..20842)
                     /locus_tag="MJB72_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBY8262770.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(20845..21246)
                     /locus_tag="MJB72_001655"
     CDS             complement(20845..21246)
                     /locus_tag="MJB72_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY8262771.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
BASE COUNT         8383 a         2199 c         3859 g         6944 t
ORIGIN      
        1 gatatgaaaa gctaaaaaaa taaattgcaa atgaaattaa aatgaataaa aaataaaaaa
       61 ataatcataa gttagaggta tcaatgaaaa ataagactta atgtttttaa aattaaaagt
      121 atactaaggg gggcattaat atgtgcgtta tatcaaaaga tagagcaaag gaaataaaaa
      181 tagtagatac tactttaaga gatggagagc aaacagctgg ggttgtattt gcaaatagag
      241 aaaaaataat gattgctgaa atgttaagtg acctaggtgt tgaccaaatt gaagttggta
      301 tacctactat gggtggagat gaaaaaaatg taataaagca catatgtagt agaaatttaa
      361 aatcagatat aatggcttgg aatagagcag taataaaaga tgttgaagaa tctatttctt
      421 gtggtgttga tgcagtagct atatcaatat cagtatctga cattcatata gaaaataaat
      481 tgagaacttc tagaggctgg gttttagaaa atatggctaa aacagttgaa tttgcaaaga
      541 aaaatggatt atatgtttca gtaaatggag aagatgcttc aagagcagat atagacttct
      601 taactgaatt tataaatgtt ggaaaacaag caggggcaga tagatttaga tactgtgata
      661 cagtaggagt tatgaatcct ttttctataa agaatgcaat agaaactctt tatgaaagaa
      721 ctaattttga tatagaaatg catacacata atgactttgg gatggcaact gcaaatgcac
      781 ttgcaggaat agcagcagga gcaaattatg ttggtgttac agtaaatgga ttaggtgaaa
      841 gagcaggaaa tgcagcatta gaagaagttt taatggcact taagtgtgtt tataaatgtg
      901 atttaaataa tatagatact agaaaattta gaggaatatg tgagtatgta gcacaggcat
      961 caggaagaat acttcctaca tggaaaccag tagttgggga taatatgttt atacatgaat
     1021 ctggtataca tgcagatgga gctttaaaag acccacataa ctatgaacca tttgacccaa
     1081 gtgaagtaaa tcttgaaaga aaaatagtaa taggtaaaca ttcgggaaga gctgcagttg
     1141 taaataagtt gagtgaatat gaaatgtata tttctccaga aaacgctacc aagctattaa
     1201 atgctataag agctacatct ataagattaa aaagaagttt aatggataaa gaaattcttc
     1261 aattatattg tgatatacta gcacatgaga aaggcacaac agaagaagaa gcagttagag
     1321 gttcatatat ttaagatata attgtttagg aggaaaatat gggagataat atagtttata
     1381 aaataattaa aaaacatata gtagatggag aagctgttgc tggaagttca attggtataa
     1441 aaatagacca aactttaaca caagattcta ctggaacaat gacatatctt caactagaag
     1501 ctatgggaat agataaagta aagactaaaa gaagtgtggc atttgtagac cataatatgt
     1561 tacaacaagg atttgaaaat gcagacgacc ataagtatat acaaacagtg gcagataaat
     1621 atggagttta tttctctaag ccaggaaatg gtatttgtca tcaagtattt ttagaaagat
     1681 tttcaacacc aggagataca cttttaggtt ctgatagcca cacacctact gctggaggtg
     1741 tgggcatgat ggctattgga gctggtggat tagatgttgc tttagccatg gcaggaggag
     1801 catactatat aaaagcacca aaagtctgta aggtgaatct agttggaaaa ttaaataata
     1861 tggtatcatc aaaagatata atattagaag tgttaagaaa acaaactgta aaaggtggag
     1921 taggtaaagt ttatgaatat ggtggagaag gtgtcaaaag cctatctgtt ccacaaagag
     1981 ctacaataac aaatatgggg gctgaacttg gagcaactac ttcaatcttc ccaagtgatg
     2041 aaaaaacact agaatttttt aagagtcaag gaagagaaga cgcatgggta gagctaaaac
     2101 cagatgcaga tgcagtttat gatgaagaga ttactataaa cttggatgaa ttaaaaccat
     2161 tagctgcaaa acctcatagc ccagacaatg tagatgaagt tgaaaatata ggcaagatta
     2221 aaatagacca agtggcaata ggaagttgta caaattcttc ttatgaagac ttaatgaaag
     2281 tagcacaaat cctgaaagga aataaagtac ataaggatgt gagtttggta atagcaccag
     2341 gttcaagaca agttatggag atgattgcta gaaatggggc tttggcagat ataataagtg
     2401 ctggagctag aatactagaa aattcttgtg gtccatgtat tggtatgggt caatctccag
     2461 gtactgactc tgtgtcttta agaactttca atagaaattt ctatggtaga agtgggactt
     2521 tgtcagcaca agtatattta gtaagtccag aagtggcagc agtaagtgca atcaagggag
     2581 tgcttacaga cccaagagaa tttgatatta aatttacaaa tttagatgta aatgaatttt
     2641 tgatagatga tagcatgata attaaacctg ctgatgtagg aagtgatgta gaagtagtta
     2701 gaggacctaa tataaaacct ttccctctta atacagagct aagtcagtct ataggtggaa
     2761 aagttatatt aaagacagaa gataatataa ctactgacca tataatgcca tcaaatgcta
     2821 agttacttcc ttttagaagt aatataccgt atcttgcaaa ctattgtttc aatactgtag
     2881 atacagaatt tcctcagaga gcaaaagaca ataatggtgg atttatagtt ggtggagata
     2941 attatggtca aggttcaagt agagaacatg cagctttagc accattatat ctaggtgtta
     3001 aaggggtaat agttaagagc tttgcaagaa tccacaaggc taacttgata aatagtggta
     3061 taattccaat ggagttttgt gatgagaaag attatgaaaa tatatctctt ttagataatc
     3121 tggagatacc aaatatttta gacaatctag gtagtggaat tttagaagtt aagaatacaa
     3181 ctaaaggaac ttcttttaag gtcaaagtag aattgtcagc aaaagaagtt gatgtattaa
     3241 aagctggtgg aaaattaaac tacactaaaa atcaagctaa ttaatatgaa aagagggaat
     3301 attgaatgta taaagtgaca cttataccag gagatggaat aggaccagaa gttgcgaaag
     3361 ctatgaaaaa ggtagtagaa gctactggag tagaaataga atgggaagaa gttaatgcag
     3421 gagaggcagt tattgaagag tatggtactc cacttcctga atatattata gatagtataa
     3481 aaaagaacaa aatagctata aaaggcccaa ttactactcc tgtaggtaaa ggatttagga
     3541 gtgtaaatgt aactttaaga caagcattag atttgtatgt caatttaaga cctattaaaa
     3601 gttttaaggg gataaaatca agatatgaag atgttgattt agtggttgta agagaaaata
     3661 cagaagattt atatgctgga atagaacata aaataggtga ttatgcagct gaaagtataa
     3721 agataataac aagaagtgca agtgaaagaa tcgttgattt tgcatgtaac tatgttaaag
     3781 ataataagag aaaaaaagtt acagctatac ataaggcaaa tataatgaaa atgtctgatg
     3841 gattgttttt agatgtgttt agagaagttg catcaaaaca tggtgtagaa tatgatgatt
     3901 tgattgtaga tgcagcagct atgaacttag ttttaaatcc agaaaattat gatgtaatgg
     3961 taatgccaaa tctttatgga gatatactat cagatttagg tgctgggctt gttggaggtt
     4021 taggtataat tccaagtgca aatattggta aagattgtgc gatatttgaa gctgttcatg
     4081 gaagtgctcc acaaatagca ggtcaaaata aggctaatcc aacagcactt attcaatcat
     4141 cagtaatgat gcttagatac ttaggtgaat atgaaaatgc acaaaaaata gaaacagctt
     4201 tagaaaaagt atttcttgaa ggaagtaaat tgactgtaga tttaggtggc tcagcttcta
     4261 ctacagagtt tgcggatgag gtgtgtaagt atattgtata gattttattt agggaaaaag
     4321 taaaatatag attgaagaaa agaggctttc tcaaaataga gataagcctt ttttattgga
     4381 atgataaaat atatttgatt ttatacaaaa gagagttcct cataaacgaa aaagttaatt
     4441 ttgggatatc cattttaatt ttaaaattaa ttgattaata tgcaaagaaa gattaaataa
     4501 atttattatt tttaattgaa ctttcaacca aaatatggta aaatttaaaa aagttgaact
     4561 ttcaactaaa aatgaatata ttgattatgt gaaattctat aaaacagaaa ctatttgact
     4621 ttagccatag tagtttcagt ggaggtgtgt ttgattaaga ctttagacag taatatatta
     4681 agagaagtag gtactttatc tagagcagta aattcaataa atgatattaa gtacaaggaa
     4741 ttaaagttac aaaaaggaca atttacattt ttgacaagaa tctgtgaaaa tcctggtata
     4801 aatcttgtgg aattatcaaa tatgttaaag gtagacaagg ctacaacaac caaggctata
     4861 caaaaattaa taaaggcagg atatgtagat aaaaagcagg atgagtttga taaaagagga
     4921 tataatttaa ctccaactga taaatctttg gaagtttatg agttgataat tgaagaagag
     4981 aatagaagta tagagatatg ttttgataat tttacagatg aagaaaaaca agtggtaact
     5041 aagttattag aaaaaatgag caaaaacgta gaaaatgaat ggtttaaagt aaagagatag
     5101 taaataaata cattaactta aggagatatt tttatgatta gaaaagcgaa tatgaatgat
     5161 ttagaaagca ttatgaaaat tataaaatct acagtggaag aaatgaagac ttataataat
     5221 acacaatggg atgaaaacta tccattagaa aaagactttg tttcagatat taagaaacaa
     5281 gatttatata tttatgaagt ggatggagaa gtagcagggt ttatatgtct taattatgaa
     5341 gagccagaag aatatttagg cttaaattgg tcatctaata aaaaagcaat ggtaatacat
     5401 agaatggctg taaatccaaa ctttagaaag acagggatag catctaagtt agttgatttt
     5461 gctgaaaaat tagctgtaga aaacaatgta tcatacttaa agagcgatac atattcaata
     5521 aactcaaaaa tgaatttatt acttacaaag tgtggtttta taaaaatcgg agaaatgagt
     5581 tttttaggaa aggagaaatc tttctactgt tatgataaaa tattataaag agcatttata
     5641 atacaagggg taaataatag attttcagca ttagtaaggg cataatataa cttagtatat
     5701 gctattatga aagggatgat ttatatgatt ggatttctaa agaaaagaag aagtattaga
     5761 aaatacaagg atgttgaagt agaaaaagaa aaattagata aaatattaaa agctgctctt
     5821 ttagctccat catcaaaggg gcttagaaca tgggaattta tagtggtaga tgataaagaa
     5881 aagctaatta atttatctca atgtagaact aaaggtggtg gatttttctt aaaaaatgca
     5941 cctttagcta ttgtaattat tgcagataaa gaaaaaaatg atgtttggat agaggatgca
     6001 agtatagctg cttcatacat acaacttcaa gctcatgaac taggtttagg ttcttgttgg
     6061 atacaagtaa gaaatagaat gcatgatgat aatatagaag ctgataagta cattagagaa
     6121 gaattaaaag tacctagcaa gtacagtgta gaatgtataa tttctatagg atatcctgat
     6181 gaagaaaaga aagcatacag tgactctgat ttagactata aaaaggttca ttttaacaac
     6241 ttttagtata taatatgttg tattaagaca ttatattttt ttctattgtt aatatatact
     6301 taaaattgta aaaatatatc tgaattttac taacttatct aggggttatg tacaaggatt
     6361 ttaaagataa atatagaaat taatgtttat aatcgataca taacttgagt aacaaaattt
     6421 aaggaggtta aagtatgggt atatttaaaa caaaaatgga tgaggactgg aaagtaaact
     6481 acattaaaga gtttaatgaa atgagagatt cttatgagag taaacttcaa aaaaaacagt
     6541 ttgaggtaga tagtttgaaa tcagaattgg atagactaag aagctataaa aatagcctta
     6601 agccaaaaga aaaacaaatt actgatgatg atataaataa tataaagagt ttaagaagag
     6661 atggcttaag ctataaagaa atttcaaatc aaacaagttg gagcaaagca actgtaagta
     6721 gggtattaaa tggactttac gattagcata tacatggggt gatttaaatt gggatgaagt
     6781 atagttacaa aaaatgcata atagattcta ttttattgat gtttatagtt caaatattaa
     6841 ggatgatttt gaattatgta cttctaagtc aatttgaatt tactcttgaa aattttaata
     6901 ttataaactt aatttctttt acattagttg gtttatcgtt aattttattt ttaaaagata
     6961 attctttgta taacaaggtt agaaatagaa aaatcacaga agcttttgaa gaaaataaaa
     7021 acaatatact tattgaaaag tgtaagctga tactttttgt tgtggtttta tctttagcaa
     7081 taattgtaac ttattgtact aagggatatg tactttttaa tgtaactatg atgactttgt
     7141 ctgtattgat agttccaata tttgaagagt tattttttag agaatatata tggaactatc
     7201 taagtaattt tataaagtca aagagtaaaa ttatctgtat aacatctata ctatctggaa
     7261 tttataatat aggatacata gatgttataa gaaattatgt aatattgtat aacaattcga
     7321 gctacacctt tgaagttatt atttcaaaaa ttatgatagg tactgttttt ggtattgtac
     7381 ttggattagt taagtatagg tttagagatg tagggttttg tatattattg aggagcttat
     7441 ttgctatatt tattaggtag atagtataat cggtttataa ggttgtttta aatttaattt
     7501 taaatcaacc ttattttatt tgtgttttat aaagcactta tatattaata actaataaat
     7561 ttaataaaaa ttatttgaat atttttaaaa tatataaata tgtattgaca tataaatatt
     7621 atgaattaca atttatataa actaataaaa attaaataat attaatcaac ttattgaatg
     7681 gaggaataaa aatgaaattt gtgacttttt gtagtagcaa tgaagaaaaa ataggtgtat
     7741 ttaattcaga aacaaatagt atatatgaaa ttaatagttt aggattaagt aaattatata
     7801 ctgatatgaa taattttatt gaaaacgtat ctacaggaga tttagaaaaa ataaaaaaca
     7861 attcatttga aaacgcaaag tgttataagt tagaagaagt taaattatgt tccccaattg
     7921 taagacctaa aaaagatatt atatgtttag gtctaaacta taaagaccat gtcaatgaaa
     7981 tcccagatgg agttataaaa aacgtagtta tgccagatta tccaatttat ttttctaaga
     8041 gagcagacaa aataattgga gtggatgata agataagctt acatggagat ttggtagaga
     8101 agttggacta tgaatcagaa cttgcagtaa tcataggtaa agaaggtata aatatatcaa
     8161 aagaagatgc ttatgaatat atatttggat atacaatagt aaatgatata agtgaaagag
     8221 cacttcaaga taaacatgtt caatggttta gaggaaaaag cttagacaca catacatcta
     8281 tgggacctta tatagtacat aaagaagaat ttgaacatcc tttaaaatta gatataagta
     8341 gtgtagtaaa tggagaagta agacaaaatt ctaatacaga gtactttata tttgatatac
     8401 ctactataat aaatgattta tcaagaggaa tgactctaaa gccaggagat ataatttcaa
     8461 ctggaactcc agcaggggtt gctatgggga tgaatcctca agtatattta aagcatggcg
     8521 atatagttga atgtaaagtc gaaggaatag gagttttaaa aaatatagtt gattaatgga
     8581 taaatatgcc atataatttt attagtacga ataatttaat taatatagga ttaatcaaac
     8641 ttatgattaa tttattatag agagggatga gtatatggca agagaactag aattaaagta
     8701 tggctgtaat ccaaatcaaa aaccatcaaa gatttatatg aagaatggag aacttcctat
     8761 agaagtatta aatggaaaac ctggatatat aaatttttta gatgcattta atagttggca
     8821 acttgtgaag gaattaaaag aagctacagg tctcccagca gcaacttcat ttaaacatgt
     8881 aagtccagca ggagcagcag taggggtacc tttatcagat actttaaagc aaatatattt
     8941 tgttgatgat ttagagcttt ctccattagc ttgtgcttat gcaatggcaa gaggagcaga
     9001 tagaatgtct tcttatggcg attttatagc actttcagat gtatgtgata aggagactgc
     9061 aactattata gcaagagagg tttctgatgg tataatagct ccaggatata cagatgaagc
     9121 ccttgaaata ttaaaaggaa agcgtaaagg caactataat attgtaaaaa tagatgaaaa
     9181 ttatactcct gaacctattg aaacaaagga tgtttatgga attacttttg aacaaggtag
     9241 aaatgaaata ttaataaatg aagacctatt aaaagatatt ccaactgata ataaaatttt
     9301 tacagatagt gcaaaacgtg atttaattat agctcttatt acattaaaat atacacaatc
     9361 aaattctgtt tgttatgcta aagatggaca agtaataggg gtaggtgcag gtcaacaatc
     9421 acgtattcat tgtactagac ttgcaggtaa caaagctgat acttggtatc ttcgtcaaca
     9481 tcctaaagta ttaaacttaa aatttaaaaa ggatataggt cgtccagata gagataacac
     9541 tattgatgta tatctttctg atgattatat ggatgtgctt gctgatggca tatggcaaaa
     9601 cttttttgaa gaaaaaccag agcctctaac tggagaagga aaaagagcat ggcttaaaac
     9661 tttaacagga gtagcacttg gttcagatgc atttttccca tttggagata atatagaacg
     9721 tgcaaaaaga agtggggtaa gttttatagc acaaccaggt ggttctattc gtgatgataa
     9781 tgttatatta acttgtaata agtataacat agtcatggca tttactaaaa atagattatt
     9841 ccatcattaa aatactgaaa gtataaagat aatatagatt aggtaaaaat ctacaacaag
     9901 ttttgttgta gattttttta tttgctgtat aattttaaat agaactttat ttttagatat
     9961 tcaatttgga ggttgatatg gctttaaata taagaaacta tagcctatta tgtaatcaag
    10021 gaacagctaa atttaatgaa gatgtagtcg gtataaatcc atttggggca tgggttttag
    10081 atggtgctac aggacttaat ggaaaaaatt tagtgtcaac tgaaagtgac gcaaggtggt
    10141 atgttcagtg gtggaatagg tatttatata aaaatataaa taaagaagaa tcattaaaga
    10201 gtattattaa tactggaata atagaagtaa aaaaagagta tgaactaaga ttaaatgggt
    10261 ttaaggttga aaaattagat ttaccatcta gttctatagt tgttgtaaaa tttcatgaaa
    10321 ataaagtaga gtatttttta ttaggagatt gtgctttgtt tattaaaaat ggagattcca
    10381 aagttataaa agataggagt atttgtaaat ttgataatac tgtatttgat gaaatgagta
    10441 aactttctaa cttggaagag atggcttttg atgagattag aactagtgtt atggatacaa
    10501 tagtaagcaa tagattgaag aaaaatacaa aagagggcta ttggatattg gattttgatg
    10561 aggaagctgt ggaaaattct atatatggat acattgaaat aaaaaaagat tttcaattga
    10621 tgctaacgag tgatggtttt acaagtgcat gtgatagata taatctaata aaagaagaag
    10681 aattgatacg tatagcagaa aaatttggta taggatatat acataataaa gtaagggatt
    10741 ttgaagataa tgactataaa gcggttaaag tgccaagatt taaagtaaaa gatgattcat
    10801 cttgtttata tttagatata tataatgatt agactgtata aaactattta attttagaaa
    10861 ggaggcaagt gaaaagtgaa aattcttcat ataataacac aaaaaccaaa tagtacaggt
    10921 agtggtatat atttgtctgg tatgataaaa gggttcgaaa aaatcggtca taagcaagct
    10981 gtaattgcag gtatagatgt aaatgatgat gtaaattgtt ttccaagtga agtaagtttt
    11041 tatccagtaa agtataattg tggggagtta aattttcctg ttgtaggtat gagtgattct
    11101 atgccatatg aaagtacaag atataaagat ttaaatatag acatgataaa tagattgaaa
    11161 tatcaattta aagtaaatat agataaggct atgaatgatt ttaaaccaga acttataata
    11221 tgtcatcatt tgtacctctt aactgcattt gttagggaaa tggtaaaaga tataaaggtt
    11281 atgtctattt gtcatggaac ttgtttaaga cagttaaata ccattgattt agagaaagaa
    11341 tacataatag caaatataag gaaattagat ttaatatttg ctcttcatga aaatcagaaa
    11401 tatgatatta taaagacttt tggagtaagt gaaagtaaag tagttgtaat tggtagtggt
    11461 tataatgatg atatatttta taataaaaat tataaaataa aggatgataa aataaaaata
    11521 gtatttgcag gtaagatatg taaatcaaag ggactgattc catttataaa agctatatct
    11581 aaattaaaat atagtaaaga tttaatagaa gtcaattttg caggtactgg aagcgatatc
    11641 gaatcatata atgagatagt taaactggca agtaagtctc catttaaaat gaatttccta
    11701 ggaaagttag aacagagaga tttagctgag ctattcaaca ggtcacaaat atttgtgtta
    11761 ccatcatttt atgagggatt accagttgta gttttagaag ctttatcttg tggcacagat
    11821 gtaatcacta ctgatattct tggtgttaaa gagtggattg gtagtgagat taataattct
    11881 ggaaagatag aatatatttc tctacctttt atggagaaag aaggtattcc aaaagatgaa
    11941 gagttatatg attttgaaaa taatttgtat aatgcaatag attctaagat acaatcttta
    12001 ttgaataact caaataagaa aacatctgta gatatgtcaa aaaaaacgtg ggatggctta
    12061 gcatatagaa taaatgaagt tattttaatg gatgaattgt gtttagtcta aaattattta
    12121 aattataaaa aagtgtaact atttttacac tatttttaaa tgattttata ttatactaat
    12181 actaaatata tttgttttag ggaggtaatt tatgaggatt agtaaaaagg tattggcgtt
    12241 aggtatttca gcattactgt taagtgttag ttttccaaca tcaataaacg cactagataa
    12301 gattgaaaat attcaaggag tagataaata tgaaacagct gggcttattt cagataagca
    12361 agattttaca acagcagttc ttatcaatgc agattctaca atggtagatg gaattgctgc
    12421 aagtggtctt gcaggagtaa ataatgcagc tattttatta actaataagg atgatatacc
    12481 agaagcaacg cttcaaagac tgaatagagt tactaagata tatgtaatag gtggagaaaa
    12541 ttctataagt aaagatgtag aaaaaatgtt acttatgaga agaatgcaag ttataagaat
    12601 tgatggtgta gatagagtag atacaagtta taaaattgca ggcgaaatag aaaaaattaa
    12661 aaacagtgat aagatgtttt tagtaaatgg atttaaggat gaagctgatg cagtaagtgt
    12721 tgcatctgtt gcttatagag atggagctcc tataatattg acaaaagata taccttcagc
    12781 agaagaagac acagacttag acccatggtt tggagtttct cctgttccag tatatgctat
    12841 tggaggagag tctacattaa gtgattacat agtgagcaga tatagagcaa ctagaattgg
    12901 cggtgttgat agatatcaaa ctaataaaaa tgttattgaa aagttctata atggagcaaa
    12961 agagttctat ataacaagtg gagacgattt agtttatgct ttagttgcat caccacttgc
    13021 aaaaaatgct cctgttgttt tagtttcaaa taaaagtgat aagagtatat taagtggagc
    13081 tagtaaagta acagcaatag gtataagtga taagagtatt atagagcaat gtttggatgc
    13141 tgtaaagaag taatggagtg ttattagtaa aatgaatata cacattgaaa attagtagat
    13201 tgttcatttt ataatggtat aaaatatatg ggaagaggct atcaaatgga gataagcctc
    13261 tttttcattt atttttttgc gtcccttatt ggtaataaaa gcttcccctg ctaaaattct
    13321 tccccaaaat acggtttctt cttggttggt tttttttaca gccatttcag atatttttat
    13381 caattcacag tttgcttcaa aaaatacggt tttcaaccat gagtgaaatt ctgtcagagt
    13441 atcttgtgta acatcatgat gacaaacttc cgtagatatt accatattaa atattttcaa
    13501 ttttgggttc attccacctg ctgtccctga attgagaacg atatttactt caaaaaggtc
    13561 aatactttag tttccaaaaa acggggattt attgctttcg tttacttttt cgatttttcc
    13621 agacattgta taaaatatct ttaagaatta acattgcttc cagttcatta tattggtatt
    13681 cctgttttaa ttcgtccaac atcatgttaa ccttataagc ataattagaa atattaacat
    13741 ctgtctgata tttaagaaga atagggtatt tttttcccca ggcatttgtc caattgattt
    13801 tttcttcggt ttcctcactt gttttattaa tcgcttcctg aatttcttcc atgtcaatat
    13861 caaaatcaat caattcatct aatgacacat tgtaaagtat tgctaatttt tttgactgac
    13921 gaatgtctgg gactgtttca tccgtttccc attttgaaac agtctgcctg cttaccccca
    13981 gtttttcagc tacatcttcc tgtgaaagcc catgtttctt ccttgcctga aataaactat
    14041 ttcctaaatt cattgaaatt cctccttatc tgtttatggg attattatat tatttgataa
    14101 gaacaaactc taccaactaa aattatcata tttgctattt ttattaacat aattcaaaga
    14161 cgtatttatt ctgcaaatta aaatctgcta acctctgcga atatcaatca ttaaccactt
    14221 ttatccattc cctgtttttg tttgtacgta aatatctctt aattctggtg taaactcctg
    14281 taaaatcgct tcgacaacct ctttctcttt ctttcaaaca acaactaaaa cgtaaaataa
    14341 ttttctttcg gatatactgt gcataaattt tttctaggtt ttttaaattt gatattccag
    14401 ccaaattccc aactgcaaga actaaatatc tattttgaga cactatctcc ttttcttata
    14461 ttatattatt ttttatatgg aataaatttc ataagtatta tcttccttta atttttctaa
    14521 ccaaaccatt tctaaaatat ctagttcttc taaatagtct ttctctgttt tgttatcatc
    14581 atgttttata agctctaaaa cttcaaatgt aaatgctttt tcaccatatt ttttccattc
    14641 attatttaat tcttttatca tacatgagcc agcaccaagc tgaaatttga gagagttaaa
    14701 tttgcttttt atatcattac tttttccaag gtaaatcttt ttagtaatat cattttttat
    14761 tatgaataca cctgtgtcaa atttcatttc tttgtataat tgttttaatt cacgttttct
    14821 atccatgatt ttagatacct ctctttataa ttgatatgta atatgtaagt gtaaaaatat
    14881 attacatatc aattataaag agattaattt tatatgtaaa tagtttttat aaatttaatt
    14941 atgaattagt catcaataaa caagaaattt agctgtagaa atccattata aaggctatta
    15001 taaattttaa tatgctaaaa atataaaaag aatagttgac agtgtagaaa atatggaata
    15061 aactaaaagt gtaatagtaa tattaaatta tatattacat tgaaaggtgt gaatgattat
    15121 tatgaataaa aaaatagaat taatagggaa ttttgcaagt gattggatta ggtatagtga
    15181 ttatgaatat aaaattactg aagaagatga attatatata gttccaacag aagatgctgt
    15241 gtttaccatg tataacccat ttgatgtagc agatgatata atagtagata taattcgcat
    15301 aggacaagag gcattacaag atatatcaaa agagacaaaa gaaatgaaac taaagaaaga
    15361 aatacttgaa tttgtaaaaa agtatggatt attgggttta atatattcaa gtgtatataa
    15421 tagagatata gttggagaag aaagagtact gatgattgaa aagaattata ttacaaaaga
    15481 gaatatattg agtacagatg aatatgtaaa catgtttata ccatttgcaa gtgatgaaga
    15541 tgtgtatttt aaaaagtata gaaggggtat agatgtatca aagagagaag aatctccaaa
    15601 gttttttgga aagagaccat tgattttaga tttagtattt tcaaagttct acactgaaca
    15661 actagattgg attgtaagtt ttgctaaaga tatgtgtaag cattttaatc aaatttgtgt
    15721 atataaaaat acatctaatc tattaactga aagtgttact attatgtctg gagggtttaa
    15781 tcctcaaaag ataggatttt ctataagcca attagataaa ccaattattg catgggagtt
    15841 tgattctttg aaaacagcta ttgagactat ctatgccttt gcaattacaa gtgaaccttc
    15901 ttctttaaat aaatgcaagt actgtggtaa aatttatatt cctacaaatt ctaaatctca
    15961 atactgttca ccttcttgta gaaattgctc aaatgtaaag aaaagtagaa gtagagccaa
    16021 aaaagataaa taaaattaat aaatatatca ttgtttagat tttagtaaat aactactgaa
    16081 ttaaaagttg ttatataaaa ttatatatct aaatgatggt attttttttg ttgagaaaat
    16141 tgaaaagttt aattgagata gtaattttga gttgacaaaa accactttta acactactat
    16201 tataagtggt tggtaaaata atatatacta attcaaatat agcataaaaa acaaaaaaag
    16261 tcaatggaaa aaataaaaaa ggagaattta aaaatgatat cacctattat tttgagttct
    16321 ataaatcaga atttaaagga aatagaaaga aatgaactgc ttgaaactaa tatagaatct
    16381 ggagattatg gtctagctct ttctgaatct gatgtaaaag atataataaa ttcgagagat
    16441 aatactttaa aggggtatgg aagaatagaa ttagatataa aagttacaaa acagttaata
    16501 gaaaatatat atacatcaca atatacaaat gtggacaatt atttagaggc tattaatgat
    16561 atgcaagaaa ttttttatta tttaaaaaat gaaactgatg acaagatatg tgatgatgag
    16621 attatagaaa tactgggtga tttttatgaa aaattttcag gcaatatgga caatgtaaga
    16681 ggagaagcag acgaatttgc aaagaaattt aaatttggag aagtttaata tatgatagga
    16741 gagattttgt atgttaaata atttaagtaa tattaatata aacgaaaata atttaataaa
    16801 gaaccaatat tccatatctc tacttaagga atgtctaaat tgtaaagtga tagatgaaag
    16861 agaagtatat aatatacaac aagaaatatc tctaatactt atggatttga ttaaaaaata
    16921 tacaaatggt caaagcacat cagtaaaaac agaagttgcc gaaaaactgt tgatttcaat
    16981 atggtatgct attgatgcat atataaataa gcttggagat atagaaaaaa gagttgtgga
    17041 gttgactgct aagaatgtta aaaaagtgta ccttggagga attgaaatat tagaggaaga
    17101 gttagttagg ctaaaagatt tttataaact aatgatgagt agaaaattgg atactaattc
    17161 aatagcatat aatgatacct tgatggagtt atcaagtttc tttaaaagtt atattgtaaa
    17221 atttgaagct caggacgttc cagtaagtat agattatcca ttggcactag atgatatgga
    17281 tgttaagggt atttattatg tgaagaatta tattgagagt atagatatag aaaatagatt
    17341 ttgtaaccta tttaagaaaa aagatagaga aaaattattt tatgactatg gggtgactta
    17401 taaaatagat tgtgatgtta tacttgtaaa tttatttgaa cttattatga ataattgtat
    17461 tttttctact attttaggaa atgaagtgac taatttagaa atttcaaaat atgagtatga
    17521 atttttagaa aaacaattta aaaatatgta caataatatt actgaagata aagttataga
    17581 ctctgaagaa agtaatataa ttgaattgat tgaagtagat gatagtttta acactgaagt
    17641 agatagtaat ttggattata atgattttga agaaattaac agagaaatta gaataaataa
    17701 aaaaataact ttactactta tggaagcaat tgaagtatta ataaaaagat taagcataga
    17761 agatgaagat gtaataagat atattaaaaa atatgaaaag atatttattg aatctgtgct
    17821 aagctcaata aaaagtaaca cttttaaaag tatggtaact gtgagtaaaa ataaaaatga
    17881 acacttagaa gattatattg ttgatgatga aagtaaattg gatgatgaat cgtttagaga
    17941 gatattcaat gagatattag atagtacatc tataattgaa aaaataagaa taataaagga
    18001 aaatatcaat gctaaaaaag actttatcga tattcttgaa tctgaatgtt tatttggaga
    18061 agagtattta atgttgtttg catcattaag tgaattagaa ttggcaatat tgggtagtgt
    18121 agtattttat gaagatatta gaatgagaga aattaatata ttagaattta tgttaaatga
    18181 gaaaactgaa actactttat ggaagataga gtacattaaa tttatgaaga ggcaaagtga
    18241 agataagata tactccatag aagagcatat gaataaaatt aattaacaaa aaattgtgaa
    18301 taaatagaat gttagtatat aatataatct gtaaagcata tcaaaaatac atatatttgg
    18361 aggaaaatta tgaaagagta tatcataaat aatgttgaag aaataagaga aaaattaatt
    18421 gatttatcta agaaaatatg ggaaaatcca gaattagcct ttgaagaaaa gtatgcaagt
    18481 agcattcaaa aagaatacct aaagtcaaaa ggatttaaaa ttgaagaagt agaaaattta
    18541 cctactggat ttatagcaag ttttggagag ggtaaacctg tgattggcat acttggagaa
    18601 tatgatgctt taccagaact atcacaatgt gtaagtgcag aaagaaagcc attggtagaa
    18661 ggaaaagctg gacatggatg tgggcataac ctgttgggtg tagcaggtgt aggagcagtt
    18721 gtttctataa aaaaattaat agaagaaaat aaatttaatg gaacaataaa atattttggt
    18781 tgtcctgctg aagaagaagg tggaggaaag acagttatgt gtataaatgg ctgttttgat
    18841 gatgtagatt gtgcatttac atggcatcct tttgatataa atgcaccttg gagaggagga
    18901 agtctggcaa acttatctgt aaaatttaaa tttaaaggga taacagccca tgcagctcaa
    18961 gctcctcata atggtagaag cgcacttgat gctgttgaaa ttatgaatgt tggagctaat
    19021 tatttgagag aacatgttat tgactctata agaatgcact atgttataac taatggtggt
    19081 ggaagaccta atgtagttcc tggatttgct gaaagttggt actttattag aggtaaaaaa
    19141 gcaaaagatg cagaacacgt tcttgataga ttaataaaag tagctcaagg agcagcaatg
    19201 atgacagaaa ctgaaatgga gtataaggtt acagatggaa tatatgacta tataccaaac
    19261 caatgtttga ctgatttagt ttatgacaat atggtttttg ttggttgccc taaaaatact
    19321 cctgaagaag aagaatttgc aaaaaagttg tgtgatactc ttactagaga agaaagatta
    19381 ggtgtagcaa catcattaca aaatgataaa tctattgtag aaagctatat acacaaggaa
    19441 atagttgacg gtgacaaaga taaaggacta gctggttcta ctgatgttgg agatgtaagt
    19501 tatgtaatac cagtggctca atttgcaatg gcagcttggc ctgttggaat tgcaagtcat
    19561 acatggcagt catgttcatc agcaggttca aatataggat tttctgctat gataaattca
    19621 gctaaagtac tggcttgtag tgcttatgat gtatttatgg atactaaaat tattgatgaa
    19681 gcaaaaattg aatttgataa atcattagat ggacaaaaat ttaaaccatt ggtataattt
    19741 tggtaataaa atatttcagg ggggaatagc tatgagagaa aaagttgaaa aagtgcttga
    19801 ggaaaagata aaaccagtat tacaaagaga tggtggagat gtagagctta tagatgtaaa
    19861 tgaaaatggt gtggttttag ttagactaca aggggcatgt agtgggtgtc caggagcgac
    19921 tatgacaatc aaagctatta ttgaaaatgt tttggtgagt gaagttccag gagtaactca
    19981 agtattaggt gtttaatagt tagcgtttat taaatgtatg ttatacataa gtatttaata
    20041 aagaagtaaa aaaaatagtg aaacaatata tatgaaaaag tatgagaaag ataataaaac
    20101 attatagaaa taatgaaata tttcatatgt atatgaagaa ataagaaaaa tagtgaagca
    20161 atatatatat gaaaaagtat gagaaagata ataaaacatt tatagaaata atggaatatt
    20221 tcatatatac atatgaagaa atagtaaaac aatatatata tgaaaaagtg tgagaaaata
    20281 ataaaatact atagaaacaa ttaaatattc tatataggta ttaaggaaca tgaaagaaat
    20341 aatggagtat tataaataaa tatgtaaaag tgtgaaagaa ggatgtaggt aaatttgtct
    20401 tatacaaatt tacctacatc cttctttcac actttttatt ttactatcca gccataccaa
    20461 taaacatacc tataaaatat ggaactaacc atatagtcca tacaactata ctaaacttat
    20521 gaaagaattt tttcttttcc tcatcatctt tgtataggac aaaagtagcc caacttgcat
    20581 gaaaaagcat aagcacaatt gcaagaagac ctgtaatact atgaaggttt tgtgataaag
    20641 cagattttac ttcaaaagta tggctgttgg ctatgttact cattgtaaat gttcctaaag
    20701 tatcaaatat aaaaccaagc caaaatataa ttacatgttt cttttttaaa atttttgcct
    20761 ttctttctcc aaatacacct atagtgtaga aaattaaagc tgatgttata aatactattg
    20821 caagtattaa tttataattc ataattatct ctcctttact tttgccaata tatttctaag
    20881 attttttact aagtcactca attcgttttc atcacaattt ttaaaaatat catcaaaact
    20941 ttcttgcatt ttaattttaa aatttgtggc aaattcaaat cctgttgttg taaacttaac
    21001 gtatgtattt cttttatcat tagatttctt gaatttttct atatatccaa tctgttctaa
    21061 tctgctgatt atgcctgaaa ccgtaccttt agctaaagac atttcatcac atagttgtga
    21121 tattgtaagt tcttgattat gagctatcag ttttataact ataatctgtt ggtgtgttaa
    21181 tccattttct ttaaaatttt cttctactgt gtacatagtt ttagaataaa gttcttttaa
    21241 gagcatggct attctaaaac agtcataatt acttttcaat aaaatcacct cttaaatggt
    21301 ttgcatacaa actaatataa tatacttttc ttatatagtc aacctaaaat tttataattt
    21361 tatagaaaat aatgaagaat agaat
//
LOCUS       DAGSHT010000050        20649 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGSHT010000050 DAGSHT010000000
VERSION     DAGSHT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.051"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..322
                     /locus_tag="MJB72_001656"
     CDS             <1..322
                     /locus_tag="MJB72_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY8262772.1"
                     /translation="LIYINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQN
                     PNDSRAFQLRVTDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTA
                     VEEE"
     gene            324..1418
                     /locus_tag="MJB72_001657"
     CDS             324..1418
                     /locus_tag="MJB72_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBY8262773.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1506..2036
                     /locus_tag="MJB72_001658"
     CDS             1506..2036
                     /locus_tag="MJB72_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8262774.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2363..2722
                     /locus_tag="MJB72_001659"
                     /pseudo
     CDS             2363..2722
                     /locus_tag="MJB72_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(2972..4348)
                     /locus_tag="MJB72_001660"
     CDS             complement(2972..4348)
                     /locus_tag="MJB72_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262775.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4616..5788
                     /locus_tag="MJB72_001661"
     CDS             4616..5788
                     /locus_tag="MJB72_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8262776.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVNSMFERVKLISTVILNLDKFEV"
     gene            5802..6449
                     /locus_tag="MJB72_001662"
     CDS             5802..6449
                     /locus_tag="MJB72_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262777.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6451..6960
                     /locus_tag="MJB72_001663"
     CDS             6451..6960
                     /locus_tag="MJB72_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBY8262778.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7324..8436)
                     /locus_tag="MJB72_001664"
     CDS             complement(7324..8436)
                     /locus_tag="MJB72_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBY8262779.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8734..9000
                     /locus_tag="MJB72_001665"
     CDS             8734..9000
                     /locus_tag="MJB72_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBY8262780.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9245..10972
                     /locus_tag="MJB72_001666"
     CDS             9245..10972
                     /locus_tag="MJB72_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262781.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            10972..12762
                     /locus_tag="MJB72_001667"
     CDS             10972..12762
                     /locus_tag="MJB72_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262782.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(12924..13232)
                     /locus_tag="MJB72_001668"
     CDS             complement(12924..13232)
                     /locus_tag="MJB72_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262783.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13494..14042)
                     /locus_tag="MJB72_001669"
     CDS             complement(13494..14042)
                     /locus_tag="MJB72_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262784.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14145..14816)
                     /locus_tag="MJB72_001670"
     CDS             complement(14145..14816)
                     /locus_tag="MJB72_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262785.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15212..16117
                     /gene="garR"
                     /locus_tag="MJB72_001671"
     CDS             15212..16117
                     /gene="garR"
                     /locus_tag="MJB72_001671"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBY8262786.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGIDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            16253..17635
                     /locus_tag="MJB72_001672"
     CDS             16253..17635
                     /locus_tag="MJB72_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY8262787.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17706..19142
                     /locus_tag="MJB72_001673"
     CDS             17706..19142
                     /locus_tag="MJB72_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBY8262788.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19328..20182)
                     /locus_tag="MJB72_001674"
     CDS             complement(19328..20182)
                     /locus_tag="MJB72_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262789.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20450..>20649)
                     /locus_tag="MJB72_001675"
     CDS             complement(20450..>20649)
                     /locus_tag="MJB72_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBY8262790.1"
                     /translation="LDESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILV
                     APETDRVNLYIETCCSSFECK"
BASE COUNT         7692 a         2431 c         3396 g         7130 t
ORIGIN      
        1 tttaatttat attaataaaa ataggaattg ttctttgaat gatgtttcta cagaatttga
       61 agttgataaa gctcatacaa caagaactat atctaggtta gaacaagatg gatacataga
      121 aaaaatccaa aaccctaatg atagtagagc ttttcagttg agagtaacag ataagggtga
      181 ggaagtatta ggggatataa aaaacatatt ttcaaagtgg gataatcaca taaaaaaaga
      241 gttctcggat acagaatata aagaactggt aaagaattta catgtggtaa aggatataaa
      301 aacagctgtg gaggaagaat aggatgtatt gtaatttatt aaaaccaatt aaaattggaa
      361 atttggaatt aaaaaataga gtatcttttg cacctacaag catgggtctt aaattggaag
      421 aaaaaattaa aaagttttct gatattgcta agtcaggagt agctctgatt actctaggag
      481 atgtatctat tagacctagt tttcataagg tagctataag cctatcggat gaagatggag
      541 tcattaaata caagaaaatt gtagatgaaa tacacaattc tggtgcaaag gtatcagcac
      601 aactattttg ttcagattat gatgttaatt taattaaaga tactatgaat atgggaatta
      661 cttcacatga tgagataaaa aaaataatga atgacggagt aaaagattac ataacaaata
      721 tgcccaaaga agaaattaaa aatattataa atttatttaa agtaacagca ttgaatgcaa
      781 aaaaagcagg ttttgacatg attcaaattc atggtgatag gcttgtagga agtttttcat
      841 caagtatatt taataataga aatgatgaat atggtggaac gtgtgataat agaagtagat
      901 ttgcaagcga aataattagt agtatccgag atgaagttaa ggacattcca atagactata
      961 aatttgcaat aagacaagaa aatcctcatt atggaaatgc aggagtttta ctttcggaag
     1021 ttgagtattt tgttaaaaaa tttgaatccc taggtgtaaa cagttttcat gtaacacttg
     1081 caaatcattc aaaattagaa gatactatac caaccaataa tcatccttac tttaaagatg
     1141 aaggctgttt tttatattta gctgatgagg taaagaaaca cacaaatctt ccagtatgtg
     1201 gggttggaaa attgtctagc ccagatttta tagaaagtat tatttcaaat aatagagtag
     1261 atatggtttc tatgtcaaga caattactag cagatagtaa ttggttacag aaagtaaaag
     1321 atggaagagt agatgagatt aaaaagtgct gttattgtaa taagaagtgt gcagatgctt
     1381 tacaaacacg ctctcagttt ggatgtatac tagattagaa tatccttata aaaataaata
     1441 atttgttaag gttaatatat ttttggtgat ttagataaat atttagataa agaggaggag
     1501 acatcatgaa aagtttagtt ttatattcaa gtttaactgg aaatactaaa aagattgctt
     1561 atactatata tgatgaaatt caagaagaaa aagacattaa agatgtaaat gaattagttg
     1621 attatggtat tgactatgaa aattatgata tagtattctt aggctattgg gtggacaagg
     1681 gaatttgtga taaaaattca aaacaagtgc ttgaaaatat tcacaataaa aagatagcac
     1741 tatttggaac aatgggagca agtaaaaaag gaagttatgg agctagtata atagaaaaga
     1801 tagaatcaat tatcccaaaa gataatgaaa tactaggttc attcatatgt caaggtaaga
     1861 tagcagaagg attgaaagca aaatataaag agatgttaaa actttctcct gacaacgaac
     1921 atattcgcca acaattaaat aaccatgagg aatctcaaag tcatccagat gaacaagaaa
     1981 tatatgaagc cagtatgttt gcaaaaaata tgatgattaa agctagtatt gtgtagtagt
     2041 taaacaataa gttcatataa gatatagaat aaataattta gtaaaaaagt atagcaaata
     2101 atttgctatg ctttttttat gaaatcaaaa aaataataaa aatacttgac tgtaaacctt
     2161 gtttatacat tattttatag aaaataagat agttttaaat ataaaaaaag atatattatt
     2221 gaaaggagat attattgtat gaaaacttat aaaagataat tagtttgatt ttaagatata
     2281 ctaaatatac aattttatag agtgaaaata tttaagacta tattatagtt ataaattaat
     2341 agaatagaga ggtagtgttt aaattgcaat aacagtattt ccaaagttgc cacctcaaat
     2401 tcctatccaa tggaataatg gtgtagctaa ttatgtagat aaaaaattta tttatgccta
     2461 ccctgtcatt tgtgttgtat tcagaatcct tttacgtcca tttatatgga gatggttaca
     2521 aatacatgct ttttatagtg atactattgc agattattta acgaatttta tgtgttttgt
     2581 agcgttgtct atagaaatat tctctatatt ttttatttac aatattgcca gacatgttac
     2641 aataattatt tttatagata tacttgtatt tattgcttta ctgataatag gtgtgtataa
     2701 attatttaaa aaatacaatt aatttttaaa aataagtaat aagtgatagt gtaaaaagta
     2761 gccactgcaa attatggatt gtagtggcta ttttattatt tgtatatgaa attattttgt
     2821 ttttctaata agttacgata tattatggtt tatttcactg ataaaaggtt taggtatttt
     2881 aaagtaaaaa gttgtatatt ctaataaaat ttaaacattt tgattcttta aaattagaga
     2941 aaatacattg cttttattaa aaaattaaat attaagaatt taatttctgc atcttattta
     3001 gaaaagataa gaaatcaact ttatatattt tttttggcct tcctctttga ttatcgagtt
     3061 ttgtatttac aatagttgca attcctgctt tatataagat actcaatatt ctattagcac
     3121 ttctaagagt tatgttcata tagtctgcta atatttctgc tgacacatta tttgaatttc
     3181 tatcattaat catacacatg attttagata catttgtatt agatagaggt ataaaagaag
     3241 caactttatt ggctattgag ctatcttcaa tattattaga atttttattt gaatataaag
     3301 gacctattgt atcattagta tcatttacta taaaagtaca gtttccattc aaagaagcag
     3361 atttaccatt agcttgacga gcattttgct ctgcatgaat atttgtattt ccaatgcccc
     3421 aacctatatt taagctatat ttaatttctt ttaaatcttc tgaaataaaa aaatctgtat
     3481 agttattggt catattgttt aacatttctt tagtagtgtg tatctctatt ttcatatcat
     3541 tttttttgac ctgaactcca tagaatccat gtcttgatag agaatttaat atagtgttat
     3601 atatttgatt ttgtatctca tcatttttaa atatcaaatc ttcatttatg tactctgaac
     3661 tatttactgt aactatacaa gttgcagatt tatttttgtc taagttttgt aatgttattt
     3721 cattcaccaa agatttaaat gtattcagga ttgattctgt agatggaacc atgtaaatac
     3781 aatcatatcc aagcttatta agttcacaac taatgatgta atttctagta aaagaaaggt
     3841 taatcttatt atctcgcaat aagtctaaat gtatttccaa taatttatca tttaattttt
     3901 taaagtcata tacaggttga gggatattaa aatctttaat tgtatatgga catttattag
     3961 agtctattaa tgaatatata tcataataat tgttgtcctc tcttaaaaaa tccataaatg
     4021 attttgtaaa gtcaatatct ttttggctat ttaataattt gaataagtat ttataaaaat
     4081 ctctttcatc aagctttaaa aaatttaatg gaacttttat gtcttttata tttattataa
     4141 gttcactata tggaactata ccactgcata caaatccatc aaaattatct acttgctcat
     4201 agtatatatt aggtaaatta ttaaaaggat tctccccttt aagctcaata aatgtaattt
     4261 cacaagtatc atttaattct ttaagagctt gtttaaactt atctgacata aaataagatg
     4321 ttgtcaaaaa accaatttta tatttcataa aacctccttt aaaatttcga gagtattgta
     4381 actaatcaat caatttataa cctcatttta taatagtaga taaaacgcgt caatttattt
     4441 tacaaaagga aaattgaaag aaaaatcaga aaatataaaa aagactgaaa aatatgttgg
     4501 ctgtcaagaa taattgtgct attttgtaat taaggactag tcttttaaga tttcctaaaa
     4561 taaacttaga ttttaggaaa gaaatacatt ttatttgaaa ataaaggaga agattatgaa
     4621 tacatattta aatgaaattt ctaagtttgt tgatgagaat agagaagaaa tagtttcatt
     4681 atggaaagag attgttaata tggaaagcta tacacattgt aaagaaagcg taaataaatt
     4741 ggctaagaga ttaaagtttg agtttgaaaa agaaggttta gattgcgagt tagtagatgt
     4801 tggagataat ggtagtacat tgataggtac acttggttcc aatatagata aaaagccaat
     4861 aattttttca ggtcacatgg atactgtctt tgaaacaggt acatttggag agaatccatt
     4921 taaaataata gaaggaaaag catatggccc aggtgtttta gatatgaagg gtggtataat
     4981 aatttcctta tatgttatta aagctttaaa taaaatagga tacaaagaaa gaccaataaa
     5041 aatagtattt tctggagatg aagagatagg acataaggat tcaacaggtg cagatgttat
     5101 attgagggaa gcaaaaggtg gtctttgtgc ttttaatatg gaaactggat tagtagataa
     5161 tagtttgtgt gtaggtagaa aaggaagaat aggatgtaat atacatgtaa aaggtgtaga
     5221 aacacatgca ggtaatgatt ttgaaggtgg aagaaatgct atagaagaaa tggctaataa
     5281 gatactacgc atacaaaaat tgactaattt agatgttgga actacagtaa gtgtaagtgt
     5341 gattaagggt ggaagagtgg aaaattctat tccagaagat tgtagtataa aagtagacct
     5401 tagatttgaa aaagtagaag aaatggagaa tgttaaagaa caagttagag agatatgtaa
     5461 agaaacttac atagaaggaa cttctacaga agttaatttt ataagtgaaa tgatgccatt
     5521 tgaaactaca gaagatgtta tgagatttca tacatttgta aatgaagtgt caaaggaaaa
     5581 tggatttgga gaattaaatg ctaagagact tggaggtagt tcagatgcaa gttatttaac
     5641 tattgcaaac gttcctacaa tctgttcatt tggtgtaaga ggggaatgga accacacatc
     5701 aagagaatat gcagtagtaa attctatgtt tgagagggtg aaattaattt ctacagttat
     5761 tttaaactta gataaatttg aggtataaag ggggatttta tatggatgta acagctggag
     5821 ataataaggt aacatggaaa gggtatttat ctttatttgt attgatagta ttattttcag
     5881 gagtgtttaa agatagtcaa ggcttcctta aagcattaga ttttgctaat ctgacaggtt
     5941 catttggtac tattgcagat ggtgtagact ttatgggtaa aggaggaacg ggagcaaaag
     6001 atggtttctt atttgctctg actttgattc caactgtctg ttttgctgta ggtcttatag
     6061 atgtagttga atctatggga ggaatgaaag cggcatcaaa gatatttaat ccattgctta
     6121 gacctctgct tggtatacct ggaatagcag gaatagcatt tgtatcttca tttactagtt
     6181 cagatgtagc ctcaataatg acgaaagaat tatttgaaag tggggaaata acagatgatg
     6241 aaagaactat atttgtagca tatcaatatg ctggttcagc tgttatactg aacacaataa
     6301 atactcaagc tccactttta ccaatagcat tattggcatt aggtccaata atccttatag
     6361 agatattctg taaaatactt ggtgctaatc tagttagatt aatcataggt gtaagaaata
     6421 aaaagaataa ggctaaggag gctgtgtagt atgagtcaac aaaatactgg aaatgcaatt
     6481 gtaaatagta aacctaagaa aactgctgtt gagatattta tggggggagc aaaaaaaggt
     6541 ttttacatag gtgttgaaca gatattacca gccatgataa tgggttatgt aatagtgcag
     6601 tttttacaat taactggttt agttgatatt ttaggaaata tttttggacc agtaatgggt
     6661 atttttggac tacctgggga atctgttgta gttttgatag cagcattctt tgctaaggca
     6721 gcaggagcag ctacagctgc aaacttattt gcacaaggtc taatcactgc accacaagca
     6781 accatattaa ttatgccatg tatgttaatg ggtactctag ttggtcatta tgccagaata
     6841 gtgcttgtag caaatgccag tactaagtat agaatgttac tgttggcagt tcctatattt
     6901 gatgcaatag tggggatgtt aatagtaaga gttttactca ctgttatggg attaatgtaa
     6961 ataacataaa attataacct aactataaat gatatataag aattaatata tacaaggatt
     7021 aatgagtagt aattgatata tagtaataaa ctaagatagt aattaatgtg tatattaatt
     7081 aaaaaaagag agtatctcaa agtgaacttt ttagttcata agatactctt tttgtatgaa
     7141 atcaagtata ttttcataat ttcaacaatg aaagagaggc ttatctctat tttgagacag
     7201 cctcttcttt taaatttata tttctagaat ttatgttttt tatttatcca acattaacat
     7261 tagttattag aaataatttt aagaaaaata tactttcatg ttaatatcag aataaatgtg
     7321 tttttaaagg atgatttgtt ttgaattttc acgaatatct acttctatga ctttttctat
     7381 tgtatctcct ctaaaaccta ataaattact tgtcaagttt gcagtggaac aagaatgatt
     7441 aggtataatt cttattctat caccaatttt tatatcagtt tcaccattta ctttaatttt
     7501 tccaacttct tctgaaagtg aatcaatagt aagttctgga tgtccacata ccctaccata
     7561 accaacaata ctactatttc catgtgcacc tttatcaagt ccaaggcact tacttccaca
     7621 atcaattaaa tagtatccac tgctattttt tgagattaca gtgcacacaa cagtaagagc
     7681 acacatatct tcagtagcac aacctaaacc tatctgtata gcatcatagt atacatagtt
     7741 tccaggtctt acagcagtta tagttttaga agcagcttca gattcaactg ttggagtact
     7801 accagaagcg acaatttcta tattaaatcc attatcagtt agagctttgt atgctttctc
     7861 catagaagaa tcttcttgaa cacaaacatc tggaacacct tctggagaac tacaaccata
     7921 cacttgacct ggatgcgaac ttataccaac aatcttgatg ttttctagct tagaaatttc
     7981 agtagcaagt tcaaccactt tttctggttc aaccccaaaa cggtgtaagc cacaatctac
     8041 aataataagt agcttgcact ctatttttga tttagaaaaa gcttcactat aagccttagc
     8101 acattctata ttatctgtag cacagattaa atttattttt gatgccatag tcacaattct
     8161 atttatattt acttctccca tgtaaggata agccaacata atatcttcaa aacctgcttt
     8221 tatcagagct tcaccttcat caagtgttcc aactagaaaa ccactagcac ctgcattttt
     8281 ttgaagtctc gctatgtaag tacttttatg tgtcttaagc ataggccaaa gttgtttatt
     8341 attatttgta caaatttgtt gaattttatc tatattcttt tctaactggt ctaaatctag
     8401 caaaaaattt ggagtagcga cttctgaaat tttcatgtta tttaccacct ttcaacaaaa
     8461 gtaaattaac tataagtatt ataattatta tattagtaat atcggcttta taatattaat
     8521 agcttataat tatattatag aactagcgtt aacagagagc aatatttatt tgtaataatt
     8581 agaattttca ataagttagt gtagttttaa attatttata taatatattt ttaattttat
     8641 gcaaagttta tttaaattag tttgaggatt tattaatagg gtattattga atgagtaaaa
     8701 aagtgatgtt tacatagaat aatcacacta taaattaatt tagaacctaa aaaattaatt
     8761 gagcaaattg gaaaagagaa ggataaaaaa gatatttgga tagttggtgg aggcgatata
     8821 gtaaaattat ttatgaaaga taacttaatt gatgattatt atatatatat attgccaata
     8881 atattgggga gagggatttc actatttaag tctggtatag acaagactaa tttgaatttt
     8941 aagaaagcta gtaatattgg agaacttgta aaattggaat acagtaaaaa acaaaaataa
     9001 attacatgcc aatttgttac aatgttatga aacgaagcat ttaaaagact tttggctata
     9061 tgaattgaca ttacttgtca aatatgttaa catataagcg acttgtcgga gggtgtatta
     9121 aaaagaatat gctggagaag agagtgcgtt aactcttttc tccttttttt ataaaattaa
     9181 gcatttataa atttagtatt attacatgat tacactatct caaaaattaa gaaaggagaa
     9241 aaatatgaaa ttaattttaa gttatttaaa gaattataag ttattaatag tattaaacat
     9301 actggctata tttagttttg cattagtcga gttggggatt ccaactataa tagctaaaat
     9361 aattgacaat ggtattgcaa atcagaatat agcttacata aaacaaatgg gaatagtgat
     9421 tgttattatt tctattatag gggttgtagg tagtatattg ttgggttatt gttctgctaa
     9481 ggtatcaaca agtgtaacca gagatataag aaatgacata tttgagaaat cacaagaatt
     9541 ttcacatact gaatacaata aatttggaat ttcttcaatg attacaagaa caacgaatga
     9601 tgcatttcaa attcaacaat ttgttaatat actactaagg actgccttat taactccagt
     9661 tatgttcatt ataagtataa taatgacaat tagaaccagt gtggaacttt ctatagtatt
     9721 ggctatatca gtacctttta taattattgg agtagcaata atagcaaaag tatctcaggc
     9781 aatatcttca aaacaacaaa aaggactaga taaattaaac cttatatcga gagaaaattt
     9841 aactggaata agagtaatta gagcttttgg aaatgatgat tatgaaacag agcgatttga
     9901 aaaaacaaat acatattatg ctaatgtttc taagaaacta tttaagctta tgtcaattac
     9961 tcaaccagca ttctttttac tgttaaatat agctgttttg gctgtatttt ggatttcaag
    10021 tgagaagata aatattggag agcttcaagt tggacagttg gttgcatttt tggaatatct
    10081 tttccatgcc atgttttcaa ttatgctttt ttcaatggta tttattatgt atccaagagc
    10141 agaggtatct gcaaatagaa taaaagaact attaaatgaa gaaccattga taaaaaatcc
    10201 tgaaaatgga attaaggata ctgaaaataa gggtatcata gaatttgata atgtaacttt
    10261 tacctatcca gatggagaag cttctgtact taaggatatt tcatttactg caaaaacagg
    10321 tgaaacagtc gcttttatag gaagtactgg tagtgggaaa agtactctaa ttaacttgat
    10381 acctagattt tatgatgtaa cagaaggtag cattaagata aatggagtag atataagaga
    10441 atatgattta aaagctttaa gaaaaaagat aggattcata cctcaaaaat cactgctttt
    10501 tactggtagc atagcaaata atattagatt tgggaaacat aaggcaggag agtcagaatt
    10561 agaatattcc gcaaaagttg cacaagcata tgagtttata tcaaaaaaac ctagaaagtt
    10621 tgatgaattg attagtgaag gtggagcaaa tgtatcagga ggtcaaaaac aaagactttc
    10681 tattgcaagg gctattataa gaagacctga aatatacatt tttgatgaca gtttttctgc
    10741 acttgacttt aaaactgatg caatacttcg tgctaaattg aagaaggaaa caaaagatgc
    10801 aattgtattg attgttgccc agagaattag ttctattatt gatgctgata agattatagt
    10861 actcaatgaa ggacaagttg taggaatggg aactcataaa gaattattaa aaaactgtga
    10921 aatatactat gaaattgcaa catctcagtt aaaaaaggag gaattagaat aatgaaaaag
    10981 tctaaaggtt ttaagaaaac aattggtaga cttcttccat ttgtaaagaa atataaattt
    11041 tcctttatta tagctattat atgtataatt tcagcagcta ctatgaatgc acttgcacct
    11101 aaaactgaag gattgataat aacacaattg actaaagatg taattagtat cgcaaaagga
    11161 gtaccagggg cttccgtaaa ttttgattat gtaacaaaaa tattggtgat tttagcatgt
    11221 atatattttg ccaatgcaat ttttacgtat acttcaagtt ttctacttac gaatgctatt
    11281 caaaacacaa tgagagattt aagaaatgaa gtcgaaaata aaataagaag attgcctatt
    11341 agttattttg atagtaatag ttttggagat gtattaagtc gtatatcaaa tgatgtagat
    11401 acaatatcaa atgcattgca acagagtttt atgcaaattg taaactcaat cttagtcata
    11461 attttggctc tttcaatgat gtttacaatt aatatatata tggctcttat agctcttttt
    11521 ataattccaa ttagttattt tgtatctaaa tttgtagtaa aaaaatctca aagtagattt
    11581 tcattgcaac aaaatgctct tggaaaatta aatggaaaag tacaagaaat gtatactgga
    11641 tttaatgaaa taaaactata tggtaaagaa gaagattcta taaaagaatt taaaaaagta
    11701 aatcaagaac tttgtgaaaa tggatttaag gcacaattta tatcaagtat gatgaatcct
    11761 atggtttcat tagtaactta ctttggaatt gcagctgtgg cagttgtcgg ttctatatat
    11821 gcagtaagtg gaggaataac agtaggaaat ttacaagcat ttgtgcgtta tatatggcag
    11881 ataaatcagc cactttcaca aatgactcag ttatctacag taatacaatc ttcttttgca
    11941 gctatagaac gtgtatttga aattttagac gaagaagagg aaatacctga tgttgaaaat
    12001 ccagttaaga tagaaaatgt aaaaggaaat gttacatttg agcatgttaa ttttggatat
    12061 ggagaaaatt caactttaat agaagattta aatgcagaag taaaaagtgg tcagatggta
    12121 gctattgttg gacctactgg agcaggtaaa acaacattaa taaacttatt gatgaggttt
    12181 tatgatgtaa agaaaggtgc tattaaaatt gatggagtag atataagaga tatgaaacgt
    12241 aaagatttgc gttcaatgtt tggtatggtg cttcaagata catggttatt taatggaacc
    12301 atttttgaaa atatagaata tggtagattt ggggctacta aagaagaaat tatacaagca
    12361 gcaaaagtag caaatgttca tcactttata accactcttc cagatggata caatatgttt
    12421 ttaaatgaag aagcatctaa tatatctcta ggggaaaaac agcttttaac catagctaga
    12481 gcttttatat cagacccttc aatattgata cttgatgaag ctacgagttc tgttgatact
    12541 aggctggaac ttatgcttca aaaagcaatg agaaatctta tgaatggtag aactagtttt
    12601 gttatagcac atagactttc tacaattcgc aatgctgact tgattttagt tatgaataat
    12661 ggaagtatta tagagcaggg gaatcatgat gaattaatgg aaaagggtgg attttatgag
    12721 aagctttata atagccaatt tgcagacaaa gaatcagaat agataacata aacttttgta
    12781 taaaattttg tatcaaattc aaatattaat catataatta aaaagagtat cagtaagttg
    12841 tttttttata gatttactga tactctttga ttttaagact aaaggtattt atgattggta
    12901 cttataattc acaactagtc ttgttacatc tgataaccag aattacagta cttttctttt
    12961 ttaccttttt gttctctaac atataacaat tcgcttaact tatcaatgta ataaccatca
    13021 tcacttatgc gaattaaact agtcagataa tatccatctt tgcttctagg aatcttttta
    13081 tttattgcta aatcaatagc tgtctttgct gcttcaaaat gctttaaatg tgtatgacct
    13141 tcttcaaaac tctttctagt attataaact acataacctt ttttaacttg aaaaactata
    13201 aattctttct tttcatatat tttattaaac attcaatcag cccttctcaa gatagtttta
    13261 tagcataaca ttagaagctt ctaaaaaatt ttacaaagaa cataaatatt ttataaatta
    13321 taaatctaaa actataattt ataaagataa ctttctgatt atttatatga taattcatat
    13381 agattaaaag taaattatct ttaggttaaa caaaagtaaa ataaaagact agttataata
    13441 tttatatgaa aaagtacaaa atatagtata tgaatataga aaatttaaat tatttattta
    13501 cagtttttat ttccataagt atatttacct tcagatacct tgtcatcatg gaaatatatt
    13561 ctttcacaac ttttatctcc ccatagaatg ttttttgtta ttttatggga agtatctcct
    13621 gtaaatactt cagctacagt agtatctgta acatcacagt aaagagaagc aaatttttta
    13681 aaccaaggat tttcctcaaa tcttaaaagc caggaagctc cataggggca atgtaactct
    13741 tcaaattcag gaacccatcc tagaaatgga acatcagttg tgctaataat attgtctgcg
    13801 attaattcat ctccattttc atgagctctc tctctcatag caatagctct ttcttcagac
    13861 ctttcaaata aaacttttct taatatttcg ctagttttat cttcaccaaa ctcttcaaat
    13921 aacactttgg aaaaataaaa atataaatca ccaaaatgtt tgcatgccat acgaacttct
    13981 tcaatagcat aatctttgtc aatagtttca ttagttacaa catcttcgtt caaaaaaatc
    14041 actccttaga ataagtatta gttacaatat aattatagaa tattttttat aaattaggaa
    14101 attaatttga gatagatata tttttaataa atgaaggtat aatgctaaaa taacttattg
    14161 ttacaaaaat cttctaaatt aatgtattta ggagtttcca tatgaatgag agtagttctg
    14221 taaatatctt caatacatcc atttgcaata ttattaaaaa tatctgtgtg ctcttttaat
    14281 gttgtttcaa gccttccttc atgtgacaat gaatcgagag ctaatttttt tattctgtac
    14341 atataattgt taaacatttt gtcaaattca gtattttgag catatgaaac tatagttgtg
    14401 tgaaataatg tatcatattc tgcaaatgag tttaaatttt tatcacctaa taaaatatct
    14461 ttttgtttat ctaaaagatg ttttaattta cttatagttt ctactgctct agcttctttg
    14521 tattgacttg taattaaaaa agtacaaaat ccttctattg cagagcgtat ttggaatatc
    14581 tctacaattt catttgctgt aatttgatgg attctaaatc ctttgcttgg gattatatct
    14641 ataaatccat cttgactaag tctttgaaga gcatctctta taggtgttct agaaatacct
    14701 atttcactag caagttttgt ttcagaatat atttcatttt ctatcatttt tccagatagt
    14761 atcaattctt ttaagtagtc atatgcttgt tcttgtaatc ttttaaatgt agccatactt
    14821 aataataccc ccctaaacaa tataaaaata caaaaactta tataatttta agcatacaac
    14881 atagtgaaaa taaggtcaat cttttgcaat ttaaattgca gataagatta aaaaatataa
    14941 aaaattattg aagtgtgaag catgattaac attaaaggca gattaattaa aactaattga
    15001 aattagttaa aatacaaaac tgtaaaagta gtatattata tttgaaaaca atagtatgta
    15061 ttaaaactta tataggatga ctaaatataa taaatgactt taaaaattac tatttattgt
    15121 taaaataaat acttttatat tttatattga ccggtatact ggtatgtagt atactctaaa
    15181 acataaaaaa cgttttcaaa aatgtgaaga tatggagggt tataaaatga aattgggatt
    15241 tattggatta ggaataatgg gaaagccaat ggcaaaaaat ttattaaaag atggatgtaa
    15301 tcttttggtg tatgacatta ataaatctgc tgtggatgaa cttatatcat gtggagcaaa
    15361 atacgcaagt gttttagaaa tgggtcaaga atgtgatatt gtatttacaa tccttccaaa
    15421 tggaactatt gtgcaggata tattgtttgg gattgatgga ttggcaaaaa ctttaaaaga
    15481 aggaagtata gtagtcgata tgagttcagt tactcctaca gaatcaatat tgtgtgcaaa
    15541 taaattaaaa gatatgggct tagaatttat agactcacca gtaagtggag gggagccaaa
    15601 agcgattgat ggtacacttg cttttatggc tggtggtaag gaagaaattt ataagaaagt
    15661 agagccattt tttaatataa tgggaagctc atctatttta attggagata atggtagtgg
    15721 aagtgttaca aaacttacaa accaagtaat agttaatttg acgatagcag cagtttctga
    15781 ggcatttgta cttgcagcaa aagcaggtgc agacccagaa aaagtatata aggcaataag
    15841 aggaggccta gctggtagta caatattgga tgcgaagata ccaatgatta tgaatcgtga
    15901 ttttaaacca ggtggaaaaa tatctattaa tttgaaagat attaagaatg taatgcaaac
    15961 agcacacaat ttagatgtac cgctacctat gacaagtcaa ttattagaaa ttatgcagac
    16021 attaaaagta catggacatt tggaagatga ccatagtgga atagcacagt attttgaaaa
    16081 gttggcaggt gttgaaataa aaaaacacga taattaatat ttaaatatat caatataatt
    16141 aaatacatat tattaggatt agattatata aatacagcga ggttaagtta tcatagtaaa
    16201 agaaaaataa aattgataaa taataaagta gaatgataaa ggggtaaaga ttatgattac
    16261 aggatatgga cttttgatag catttgttat atctatagga attttacttg tatcgattat
    16321 taaatttaaa gtaaatccgt ttttagcatt actgataaca tctataatta caggctttat
    16381 ggttagaatg cctataaatg aaatatcaac cacaatatcg actggatttg gaaatacatt
    16441 aggtagtata ggtattgtaa ttggacttgg aataatattt ggaaatattc tttcagaatc
    16501 aagagcaaca gaatctattg caaaggggtt gttagctaag actggagaaa aaaacagtgc
    16561 tttagcagta actacagcag gttttttaat ttcaattcca gtttttatgg atgcagcttt
    16621 tgttataatg atgcctataa ttaagtatgt ttcgagagta actaagaaat ctctaatggt
    16681 ttttgtatgt gcattaggag ttgggactat agtaggtcat gcacttgtaa tacctacacc
    16741 aggaccttta gcagttgcag caaatgtaaa tgcaaatgta ggtagtttta tactttattc
    16801 tataattgtt gcttttccag cagctcttat aggtggatgg atttatggaa aacgttttga
    16861 aaaatatcca gcttatgcta ttgatgagaa tgatagagaa aaaaatttag agcagggaaa
    16921 agatagcata aaaattgaag atgatgattc ttctaaagta ccaggttttg gaatttctat
    16981 gttttcttta ttgttcccaa tacttcttat acttatatca aatgtattta gtatgttctt
    17041 agaaaaaggt tctacaatga gtggagtact tgcatttata ggagataaaa acatagcaat
    17101 acttcttgga atattggtgg caataggatt tttaaagaaa tacataaata aacctatggg
    17161 agatgtagtt attgaagctg ctgattcagc agggctaata ttattgataa caggttcagg
    17221 tggtgcgttt ggcagtgtta taaatgcaag tggtataggt aacttcttag ttgatactat
    17281 gtctggtctt agtatatcag ttgtagtgct tggattctta ttaagtgcct tgcttagaat
    17341 ttctcaaggt tctgctactg tagccttagt aacaacatca tcaattttag gacctacaat
    17401 tttggcaact ggaatgtcac cagttttagt agggcttgct atatgtgctg gaggagttgg
    17461 tttctccctt ccaaatgatt ctggattctg ggtactttca agatttagtg gattatcagt
    17521 taaagatact ttaaactctt ggacaattgg aggaaccatt gctggagttg tagcatttat
    17581 aatggtttta ttgcttagtg ttataaatgg aattatacca cttccaggtc tataaataac
    17641 aaactattgt ttatatcata taaattacat ttagactata caaattacat aaaggggcgt
    17701 tttaaatgag agaagtaaaa ccaattagca tagatatact taatactttt aaacaagtag
    17761 atgaggacag attaaataaa cttcttgcag atgagcttaa gcatttagat agaaaaattg
    17821 ttgtattaga tgatgaccca acaggtgtac aaacagttca tgatatttct gtttatactg
    17881 attgggataa ggatagtatg gaacaaggtt ttaatgagaa aaatagtatg ttttttatac
    17941 ttaccaattc aaggggattt actgttgctc aaactacaaa agcacataaa gaaatttcaa
    18001 agaatattgt ggatgtatca aagaaagtta ataaggattt tataatcata agtcgtagtg
    18061 attctactat gagaggacat tatcctcttg aaactaattt attaaaaagc gaagtagaga
    18121 gattatcggg gaagttattt cagggtgaaa taataatgcc attttttaaa gaaggtggta
    18181 ggtttactat agataatgta cattatgtta aagaaggaga aatacttgta ccagcaggaa
    18241 tgactgaatt tgcaaaagat aaatcttttg gatataaatc atctgatata ggtgagtggt
    18301 gtgaagaaaa gacaaaagga aagtacaaat ctagtgatat gatatatatt tcgtcaaaag
    18361 aacttagaga gctaaatatt gaagctataa cagaaaaact taaaaaagct gaaggattca
    18421 ataaaattat tgtaaatgca accgactatg tagatgtaaa ggtatttaca attgcgttta
    18481 tacgagcagt aaatagtggt aaggaattta tgtttagaag cgctgctgcc ataacaaaag
    18541 tacttggcgg agtgagtgat aaggaactgc ttactaagga tgaattagta tcaaaaggta
    18601 ataccaatgg aggaattata cttgttggtt cacatgtgaa taagactacc caacagttag
    18661 aagaacttaa aaattgtaaa tatccaatag aatttataga atttaatcaa catttagttc
    18721 ttcaagaaaa tggtctaaaa aatgaggtaa aaagagttat aaaagttgta gaggaaaaaa
    18781 taagcagggg taaaactgtt gctgtttata ctagaagaga gcgttttgac ttggatacaa
    18841 atgataaaga taaacagctt atgatttctg tagaaatatc agatgcagtt accagtataa
    18901 ttggtatgct taatgtaagg ccaaatttta ttatagctaa gggtggaatt acttctagtg
    18961 atgttggtac taaagcacta agagtaaaaa aagctacagt aatgggacag ataaaacctg
    19021 gtataccagt atggatgaca ggcaaagaaa gcaaatttcc taatatgcca tatataattt
    19081 tccctggaaa tgtgggagag attagcacac ttagagaatc agttgagata ttgatggggt
    19141 agtatattag tgtattgaag tttaaacgct taaatttact gtgttaaaag taaaataaac
    19201 taaaattata aattaagatt aaaaataata gtatattaat tagggtttag agtattttac
    19261 aaaaaatatt ctaaacctta atcttatgta ttagtatttt tattatttaa cttatgtgtt
    19321 tattttatta tttatctttt ttaattgatt taatattttc tttgatttta tttaaaatat
    19381 ctgtatgctt attagagctt atgttgggga atgctttgat tatacgtcta tgaagtaatt
    19441 tgtggttaga cagcaaagta ttaagtttat tttttagctc atcaatacga tttttagcat
    19501 agcgatagtt ttctttttcc ttaagtattt caaatctatc ttccaaagta tccagtctat
    19561 tttcaactaa agattcaagt ttgttttcta tagtttcttg tgtaataagt ttgtagtatt
    19621 ctttcttatc ttttaattcc ttaattataa gattgatttg ttttaactta ccatttaatt
    19681 gtaaaattgt atggactgtg ataactaaat ctaaaacaaa ataaatttct atagatatag
    19741 caaatataaa tagcacattt ttggagatag agttaataaa gtcaaccaca ataggatgta
    19801 ctataagcat tacaatcaat gataatacac caaacataag tgagttttcc agacaaactc
    19861 ttccatgaat attaaattta tgtttagaat aatcccacca agtcatatta aatatttttt
    19921 ctaaaataaa gctcgtacaa tactccatta tgctagttat aattatacta aacaaaaata
    19981 acaaaggaat attgtgtaca aaaggagtca aggtagcaac tattgctaat gctccaaatc
    20041 catatatagg gcaaaatgga ccatttaaaa aacctctatt tacaaattgt ttttgtaaaa
    20101 ctgaacaata tgtagtttca caacaccatc ctataaagct ataaattaca aaataaagaa
    20161 attgaatata aaatgtactc ataagcacct catttctagt tagtataaca ctatatactc
    20221 gatattttat ttttttgatt aatttactaa attcaattta catattgatt ataccaattt
    20281 atagaaattt atcaaattta attacagtta gaacattata aacatggtta taataatgaa
    20341 attttattta aaataaataa gggtgtcaag tataatcaaa ctaatagttg tagatactct
    20401 acactatgtt agtttaatta catttgacac ccttatattt atactatgac tatttacatt
    20461 cgaaactaga gcaacatgtt tcaatataaa ggtttactct atcagtttca ggagcaacca
    20521 atatattatc aagtgtacat aagctaccat ctttgttgta tttacaattt tttacagtac
    20581 aaactaattc ctcacaattt ctattattaa cgttgctaga taaattacta taaatagatt
    20641 catctaaaa
//
LOCUS       DAGSHT010000051        20513 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGSHT010000051 DAGSHT010000000
VERSION     DAGSHT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.052"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(300..1022)
                     /locus_tag="MJB72_001676"
     CDS             complement(300..1022)
                     /locus_tag="MJB72_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY8262791.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1081..1758)
                     /locus_tag="MJB72_001677"
     CDS             complement(1081..1758)
                     /locus_tag="MJB72_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY8262792.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1786..2601)
                     /locus_tag="MJB72_001678"
     CDS             complement(1786..2601)
                     /locus_tag="MJB72_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY8262793.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2827..3037)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3714..5780)
                     /locus_tag="MJB72_001679"
     CDS             complement(3714..5780)
                     /locus_tag="MJB72_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY8262794.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5857..8886)
                     /locus_tag="MJB72_001680"
     CDS             complement(5857..8886)
                     /locus_tag="MJB72_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8262795.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKRIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(8939..10399)
                     /locus_tag="MJB72_001681"
     CDS             complement(8939..10399)
                     /locus_tag="MJB72_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBY8262796.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10491..10865)
                     /locus_tag="MJB72_001682"
     CDS             complement(10491..10865)
                     /locus_tag="MJB72_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262797.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(11041..11781)
                     /locus_tag="MJB72_001683"
     CDS             complement(11041..11781)
                     /locus_tag="MJB72_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY8262798.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11802..12590)
                     /locus_tag="MJB72_001684"
     CDS             complement(11802..12590)
                     /locus_tag="MJB72_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBY8262799.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12678..12768)
                     /locus_tag="MJB72_001685"
     tRNA            complement(12678..12768)
                     /locus_tag="MJB72_001685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12732..12734),aa:Ser,seq:gct)
     gene            13027..13791
                     /locus_tag="MJB72_001686"
     CDS             13027..13791
                     /locus_tag="MJB72_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY8262800.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(14072..15187)
                     /locus_tag="MJB72_001687"
     CDS             complement(14072..15187)
                     /locus_tag="MJB72_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBY8262801.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15228..16754)
                     /gene="glpK"
                     /locus_tag="MJB72_001688"
     CDS             complement(15228..16754)
                     /gene="glpK"
                     /locus_tag="MJB72_001688"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBY8262802.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGIPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            17047..18144
                     /locus_tag="MJB72_001689"
     CDS             17047..18144
                     /locus_tag="MJB72_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY8262803.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPDDCYKFLDILKSKLEIKKVI"
     gene            complement(18635..19147)
                     /locus_tag="MJB72_001690"
     CDS             complement(18635..19147)
                     /locus_tag="MJB72_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262804.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            19700..>20513
                     /locus_tag="MJB72_001691"
     CDS             19700..>20513
                     /locus_tag="MJB72_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBY8262805.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRL"
BASE COUNT         6724 a         3582 c         2142 g         8065 t
ORIGIN      
        1 tttttgtgtc gcattttgtt ataataaatt aaggttatcc ccctggtaac cctagttagt
       61 taatatatct attcccaata ccctttcata aggatatatt ttatatttat taaaaatcgg
      121 attagtaacg gaaattaatt cggtggaaaa aaagagctta ccccgagctc ttttttttct
      181 gcaaaaaatc gtaaaaataa agttaatatt aatataactt taattttaaa attaatatta
      241 accatcacta aaaaaggatg tattgaattt ctaatatcaa taacatcctt tttatataat
      301 tatagcactt ttgctaaaaa tgcttttgtt ctttcatgtt ttggatttcc gaaaacttct
      361 tctggagtat tatcttcaag tatacttcct ccatctataa aaataactct atctgcaact
      421 tccttagcaa atcccatctc atgagttaca attgccattg tcataccttc tttagcaagt
      481 tcttttataa catccaaaac ttcgtttacc atttctgggt caagagcaga tgttggctca
      541 tcaaacaaca tcatatctgg ttccatagca agtgctctag ctattgctat tctttgcttt
      601 tgtcctccag ataattgtga tggatatgaa tcttttttat ctaaaagacc aactctactc
      661 aacaaaattt ctgctttttt ttctgcttct tccttagtca tcttttttaa tttaattggt
      721 gctaatgtta tattatctaa aatagtctta tgtgggaata aattaaagtt ctgaaacacc
      781 atacctatat tttgtctaac ctcatcaata ttagtttttt tatcgaccaa attttttcct
      841 tcaaatatta tttctccacc tgtaggtatt tctagtaaat tcatacatct aagaagtgta
      901 cttttaccag aaccacttgg acctactata accattattt cgcctttagc gatttctaag
      961 tctatatttt ttaatacatt taaatctcca aaagatttac ttaaattttt tattgtaatc
     1021 atattatcac tttccttttt actattctat gttttcccta aatgctaaac atgctctcat
     1081 ctagttagac acagatagtt tcttttctaa taatccaact aatcttgata gagtaaatgt
     1141 tataacaaag taaattgctg ctgctactat taatggctct aatgctctaa atgctgcatt
     1201 ttttactata tcagcagtac gcattatctc aacaactcct attacagata ttattgaaga
     1261 ttcttttaca agtgtaataa actcatttgc taatgaaggt aatatatttt ttatagcttg
     1321 aggtattatt atatatctca tagttgacca ataatttaat cctaatgatc tacttgcttc
     1381 catttgtcca ttatcaactg attgtatacc tgatctaagt atctctgcca cgtatgcacc
     1441 agagtttact gataatgcaa atgcactagc tataaattca cttggtatac caaacaacat
     1501 agggaatctt attcctaatt gaggtaaacc aaaccatact atatatatct gtactaataa
     1561 tggtgtccct cttaaaacct caatatagat tgatgatatt gctcttaata ctttgctctt
     1621 tgatattttt gccatacaga taattatacc tattataaat ccaaagcata atgccacaag
     1681 agaaattgat actgtcacac ctgttccttc caaaaaagaa gtcccaaatc tacttaaaaa
     1741 actaaaatct aaactcataa tttcatctcc cacataactt tcttactaat ttgaagcagc
     1801 ttcagtagat gcttcattta aaaattcttc tatcttcttg ccatcttgta attcttttat
     1861 ctttttgttt aataattcta taaaatcttt gttattgtca gattttttga ttgctgctgc
     1921 cataccttct gttacatctt tcccaacttc agtatttgcc atttttattc catcatattt
     1981 tttaacattt attaaagaaa ctgcttcatt agtaacaact gcatctatgt ttccattttt
     2041 taattctgta attaaatcag gtattgcttt caaagatttt gtagttgtca tttttaaagt
     2101 gttaactaca tattcttctt ggatacttcc tgcttgaact ccaactctaa gtttctttaa
     2161 atcatcttca gtttttactt tgtctatatc tgcatcttta actataactg catttctact
     2221 taaataatat aattctgaaa aatcaacact cttttctctc tctggtgttg gagacatacc
     2281 tgcaagaaca atatcaactt ggtctgcatt taaagcacct attaatccat caaatgacat
     2341 atccttaatt tcaaccttta caccaagctc ttttgcaaat tcttcagcca acattatatc
     2401 gaatcctttt attgaatctt ttccatctac taccttatga aattcaaatg gaggaaattc
     2461 tgcactagta ccaacaacta attttccatt gtcttttatt tgttctaact ttgttttttc
     2521 tcctccacca ctacatccta ctagcgataa tgtcaatcct aatactaatc ctaaacttaa
     2581 taatttttta aatactttca ttttaactac ccctctcttt taattataat ttccctcaga
     2641 ttttaatact taaatgtttc gagttacaaa aaaagtctct cagtctttaa tgactaagag
     2701 acgactttac cgcgttacca ctcttattga taaatactta tattactttc aaaatttgtg
     2761 actgttagca tttaagctgt atggatattt ttatttttaa atcaaaaaag tcccttagct
     2821 ttacagctaa gagacgaatt atatccgcgt taccactctt tttgataagt aagtacttat
     2881 cctctcaatc ccttaaggcg ggtaaaccaa ttatcatact aaagtttctg ataatctcct
     2941 ccagagctct cttcacatag acttcattgc taggcttaca ccaaccccta actcgctgta
     3001 aatccatata tgctactttc tctatcattg gatttgtttc cttaaattgt taatacaatt
     3061 gtacttcctt ataatatttt tgtcaataag ttttttattt ttttaataat ttttacttgt
     3121 aaattatcta aatactactg tttatattta ttgatattag ttttgtaatt ataaatagta
     3181 tatttcatat ttttagctat ttcttaaata atactaaata atatctttca cattgttgct
     3241 ttttcttaaa ttacactaaa taatatcttt cacattgttg ctttttctta aacaatacta
     3301 aataatatct ttcacattgt tgcttttctt aaattacact aaataatatc tttcacactg
     3361 ttgctttttc tttaataata ctaaatagta tctttcacac tgttgccctt tctttaataa
     3421 tactaaataa tatctttcac attgttgctt tttcttaaat tacactaaat aatatctttc
     3481 acactgttgc tttttcttta ataatactaa atagtatctt tcacactgtt tctctttctt
     3541 taataatact aaataatatt ttccacattg ttgctttttc taaacaacta taaataatat
     3601 attaaaccct tatttctttt tctaataagt taaataatac attttacaca tatctatttg
     3661 tcttgcacat aataaataat acattatatt tctatctaaa tttagattct aaattaaata
     3721 gtataaatta tattcttcaa taaacaagtt ttcaaatcca agattcgctc tctatcttta
     3781 aactttattg taaacataat tccatctttt ttaattattt ctccttcatg aaatttttta
     3841 tggtatacct tatcccctac accaatactg tcaatttctt ttttcgttgg agattttata
     3901 tcattaataa ataatgattt atcaactttc ttaccatact tatttaaagt agagcttata
     3961 cataactttt cttcagctct tgtaatagca acatacataa gtcttctttc ttcttctaat
     4021 tgttcttctt ttttctcttc cttacaaata tcatatgatt tttcatgagg tattgttcct
     4081 tcatttacac ctataatata aacatttcta aattctaatc cttttgcact atgcatagtt
     4141 gtaaatatga caccctcagt ctgcttattc ttgttatttt ccgttaactc agtctttact
     4201 ctatcaatat gctctaaaaa ctctgtaaca gtattaaaat tagttgctga actctcaagt
     4261 tcatttaata tctctacaag tccattagtc tttatctttc tgttgctaca ataatctaaa
     4321 acatatctat cataatcaag actagttcta atataagata ttgcattttt aggatttaaa
     4381 gtatttaaat aacttaaatc tatttccaaa tcatttattg ttttaacttg tttaggatga
     4441 agattacact tatttattaa tgctgttata aagtcttttt catccttaac catatttata
     4501 ctgtctttgg atatatatct aaaaggcttg tttataattc tcaaccaatc tttatttgat
     4561 tttggattaa tacctattct taaataggct agtatgtctt gacttgccca atggtcatat
     4621 attgtaatta ctgagtcttt tactacaaat ggaatcctca tatccataaa tacatctact
     4681 aatgctcttg actgtatatt agttctataa attactgcaa aatctgaata ctctacataa
     4741 tctttttgta tttcgtcaat tatttccctg gcaatatata aggcttcttc ctctgaatca
     4801 tgcggagaaa tataggtaac acttcctcca tccccctgac tacacttaat gattttttca
     4861 tatctatttt tatttttgtc gataagtcta tttgcagtat gcactatctc acttttagac
     4921 ctgtaattta tatctaatat tattttttta gtattattaa agtattgttc aaattcaagc
     4981 aaaaaatctg gtctagcacc tctaaatcca tatatacttt ggtcttcatc tccaactgca
     5041 aatatattgt ttaatgggct acaaattaac tttaaaactt caaactgtac tttatttata
     5101 tcttgaaatt catctatcaa tatatattta tacacatttc taaccatttc tagtgcagac
     5161 ttattgttca aaagtaaata ataagttctt ataagcatat catcaaaatc tattttatta
     5221 actttagatt tttgttcttc gtataagttg tatactttta aaaactcatc ttttgttaat
     5281 acttctgaat taaattcatt tttatccata agttcatttt ttacatagga aatttcattt
     5341 ataacttgtc ctatattctc atcatcatca gcattctcta tatttaaact ttttaggatt
     5401 gccttgattg tcattcgctt tgacttttca tcaaatatac tgtctaagtt gtatctttca
     5461 aagtatctta gaattttaaa aaatacagag tgaaaagtac cataggttac cttattaagt
     5521 cttatgtcat cacttaaatt caaagctcta tttttcattt ctattgaaga tgccttggta
     5581 aaacttattg ccaagattct tgttggtgct atgtttttgt ttacaaccat attggctatt
     5641 ctatatgtaa ttactctggt cttacctgaa ccaggccctg caagtatcat gcaaggccca
     5701 tctatatgct caactgcttc taattgattt tcatttaact ttcttatatc tatatttgtt
     5761 gtgattatta gctccaccac cttgatttat aattatatta ttttcttttt aattataaac
     5821 taaaagttca taaaaataaa tttaattttt cctctattaa accacatcat tcctattttc
     5881 atgacatatc ttgagttctt ttttaatttt gtcgcttata tcttcaggta taaatgatgg
     5941 tttactaata tagtagcctt gcatataatc tactccaaga ttaattagtg tttccatttc
     6001 accttttgtt tctacacctt ctgctatcac tttaatattt ctttcgttag aatatgagat
     6061 aagatttttt aatattttct gtctgttttc atctttatca atacctctta caatagacat
     6121 atcaatcttt acatagtctg gcattatata taatagtaca gcatctccgt tgtaacctgt
     6181 cccaaaatca tctaaagcaa tttctatatt ccaatggtct atatatgctt tctttttctc
     6241 aataaaatct tcattaatct tatcattctc tgttatttct agtactactc tatttaaata
     6301 ttcaccatat aaatcttcaa tttcctcaaa atctactttt gacaaattat tattaggaat
     6361 agaatttaag aacagtttgc aatcgccaaa tttttctttg tattttttaa atgatttaag
     6421 tgctttaaac catgtaatac gctcaatttg atataattta gactgtgaac gagctaatct
     6481 aatgacatct attggcgaac taaaagtttt tattttaggt ctcataagtg cttcatatgc
     6541 aaatacttta cctttacaat ctacaatagg ctgaaatgca tactcaacta attgaccatc
     6601 aattaacttg tttaattctt ctctattact caataagaat gaatccttat tatatatatt
     6661 aatatcaaat tcactaactt cacccttgat tgtattttta atttgataca tagcaaaatc
     6721 tgcgtatctc attaattcat catagttggt tgaatcatca gggtaccagg aaattcctgc
     6781 tgatgccctt attttaaatt ctgttccatc atgaagtttt aataaggtat tattcatctg
     6841 tttcttaaca gaattaataa tatctcttat actatcttta ctttcatatc catatataaa
     6901 tacataaaac tcatcacctg aaagtctaga tactatacca ttccatgata taaacttctt
     6961 aagtatgtct gcagcacacc taatatattc atcaccagca tcatgtccat atgtatcatt
     7021 aatatatttt agattatcta aatcccacat aataaatgct gctatcttaa gttcatcttt
     7081 attcttaaat ttttcttgta tctgtgaatg aaaagctctc ctatttaaaa gattcgttaa
     7141 aatatcaaaa tctctttcat attctatctt ccttttctca ataaattctt ttgttacatc
     7201 aacaacaaca ccaagaacct ttagctcatc ttcaaagata ttcattctta tccacttaag
     7261 ttttttatct ttagattcaa acttatatat atatgtattt tctgtttcat aactttcttg
     7321 caaatattta tctagtcccc ttaatttttt caggaactta tttttttcca tatatccata
     7381 ttcttcaaat ttgatttcag tagtaaacac gctaaaaaat tcttttgtac aaaaaacatt
     7441 ctctgcgttt ttgtataact caaaagctcc aattggcata tctaccattt caataatctg
     7501 tgataatttt gacgaagaat ctgcaacatt tgcactgagc aactctatag ctgaagataa
     7561 ttcatctatt tcagatatat ttatcttatc aagatttact ggttttgatg gattactgtt
     7621 tctaactttg ttggctaaaa gaacaattcg ttttgtaaat aatcttgatg caatgtaaat
     7681 acctattaga cctattaata aagaaattgc tatagatatt ataactaatt tctctaccct
     7741 ttttgaaaat gacaaaagac ttttttcttc aataataccc aaaagagccc aacgttcatt
     7801 ttcaaatgga gtattggagt tatataaatc taagtattgt acactagcat aaatgttgcc
     7861 attaccacat tcattatctt tatttttaat cttatatata tttttatacg attccttctg
     7921 ctctaaatta gaatgtaaac catcccaaaa tttttctttt aatgagtatc catttgaaat
     7981 tacattttca aaggccatat cattatttct atctacacct aaaatataat tacccttttt
     8041 attcccattt atctctgagt atggaagtaa ttctcttaag taatttacac ttaaatctat
     8101 tccaagtact ccatatacag ttccatcttc ataaataagg ggaactgtat atgtcataag
     8161 cctatcacct gcatttgtca ataaaaactt acttccccag tatcctaaat tagaacttga
     8221 tacatttgga ttatcaattg cagctctaaa tggtttataa aaaaaatcat ctccatcttg
     8281 ctcattttca cttttaaatg aaaacttagg ttcccaataa ctgtccatag atattccaaa
     8341 acttcttgct atatctgatg aacctctctc aattaacaaa tcagaattat catttggatt
     8401 aaacttaggg tctaaatccc ttatatacaa tcctgtccta cttatttcac ctttactatc
     8461 tattgactta tcgttaatat ttttgtcact taggattata aaagctcctg taacagaatt
     8521 ttttctcaaa agatatatta aatcatttga tatgcttttt ataatatctt tgttaatttc
     8581 ttcattgttg cctacatcct caattaaggc atttttttca tttaatactt tttttacaga
     8641 ggaattaatt gtagcttccg tttcacttgt atttgaccat ctttgtaaca tttcattttg
     8701 tatataattt ttacgattaa ttactctttc atttaagatg tcaaacgaat tatcatttaa
     8761 tttcttaata gttcctcccc ataaaattgt ggcacaaaat aaacttgttt gaattagcat
     8821 aacaataatt aggggaaata tcatcttttt cattattgaa ttgttttttc ttagattgtt
     8881 ttccataccc attccaccta tacctttgtg ctcgtcaaca cactaactgc caattcatct
     8941 atttttttat agcattttca agctcagcct taaaactttc aaaccaagtt ttaaaatttt
     9001 tatcattatt aaattgtgct actgcatcct tcttagaagt tccacttttt actaattcat
     9061 ttattttatt tgaatcttct ttagctttat caagcattga atattcaagt atttgtctag
     9121 ctttagttcc accatcaaat gctttattag tatataaatt acaactgtta atctgttcaa
     9181 tagatgaggc tattgaacct tcaactttat cagataaact caaattatct ttcttcataa
     9241 cttcttttat aacatccaaa tcattcgttt ttttctttac tggtaagtat ccagaagcaa
     9301 tagagaattt tatattaggt tctttatctg taaaccattt taaaaattct actgatgcat
     9361 attcttcttc tttagtagat tttgttacag ccattcctgc accttgttgt acagcatatt
     9421 ttccttctcc tttaaaacgt ggtgcttgaa aaacagatgt ttcaatatta taactctcat
     9481 tatcatttag cattactcta tctggaaagt atgctgctcc tgaactagaa cctacaagag
     9541 ctaatatatc acctgttttt gcatcatcag aacgaaactt accataagaa gcaaaatatc
     9601 catttatata tggtacatag aaattatccc atagtttttt cataactttt tcatcaatat
     9661 ttagtgtaac tttaccatct ttaacagaaa aaatttctac tcctagttgt ttacttccta
     9721 taataatata atttgctaaa gcatctcttc caaaaaatgc tttaccatca ttaggctctt
     9781 tcgttaaact atctgtccat tcataatatt tttttgatgt ctctactaat ccttcaattg
     9841 tttttatatc atttatattt gaatttgtag cttttgcaaa tttatcccaa tcagttttat
     9901 ttaacatcaa aatttcagtt gattttgcaa ttgggaaaag tttaaactca tccttagaat
     9961 ttattctgcc ttcttctaca tattcgtcta tgtattcatt taattcttct ttacttatgt
    10021 acttatccat atctactagt aaccctaatt tatccatttc atatgcagta tctgagtatg
    10081 ctgcaaatat atttggtaca tcttcagaac caactttttt atttgcagaa tctaaaactt
    10141 tctctgataa atcaactaca ttaccttgac tgaatgcttc aacaataatt cctttttcag
    10201 cacctacagt gtcattaaat tccttaacaa gcttatcaaa agccattttc tgctgtccat
    10261 tataataatg ccaaatttgt attgatgtcg gattttctgg gtctaactta taattatctt
    10321 ccttctttaa actacatcct gtaagtaata aaacaatcat tgatgtgata attaattttg
    10381 caaacttaca cctattcata aaatctactc cttcaaatta taaaatttat aatagcatta
    10441 aaactgtaac ttcactattt atattaatta tagaacgttt attgatatat ttaaacagga
    10501 tttacaacat atttaaaagc aattaagaaa catactatta caaatataga aaataaagct
    10561 tttacataat ttttatttct aaatgcgcca aatgacacca atccttgttg aatagctact
    10621 gtaattacta aaagtatcat tattatatta agttgaacca gtttaaataa gtaaagcaat
    10681 gaaaacacta ctagaagtac acataataca atactagatt tattaataat atcatcaatc
    10741 ttatctacct tcttagattg cttattaact ttatttaatt tttctttatt taaattctca
    10801 acttttttat tatacctttc tcttgtttta tctttatatt tagtattttt atttttattt
    10861 cccatttatt ttccatcctc tataaatttt ttaattgagc ttatacatta tttaaatatt
    10921 atatatttaa gataccatat attcctacaa aaaggtaact tactatgtat ctatttttga
    10981 ttttcgcata aaaaagccat ctcctttaga attaattctg tattgagaca gctaatttaa
    11041 ctatttatcc tttgaaacat tttctacatt atcatattta ctatcaacta aaactatatc
    11101 aactcttcta ttttttgacc taccttcttc agatgaattt tctgctattg gtctaaactc
    11161 accatatcct acagcagata atttatctgg tgctatacct gcattgtcaa taagaagttg
    11221 aacaacattg gttgctctca taactgataa atcccaattt gacttaaact ctgaattttt
    11281 tatagacata ttgtctgtat ggccttctac tctcatgtaa ctattcattt catttaacat
    11341 cttacctatt tgaataatct tatctctaga attatccttc acaatcgctt tccctgtatc
    11401 aaatagtatt gtatctttaa ggcttattac taatcctcta tcttgaactc ttaatgatac
    11461 tgaattagcc ataccatttt catttaaata cttctggatt tcttcaccaa cttttttaaa
    11521 cttagcattt tgtaaagtat ctaaatcatt acttcctggt tcaactactg gttctatctt
    11581 gctattacca ccttcaataa ctccagaaga accaccaaaa gcagaattta gagattgaga
    11641 taattgttta tatttctccg catcaacatt actcatagaa tacattatta caaagaaaat
    11701 cataagtaat gttatgaggt ctgaataagt taaaagccat cgctcattgt tttcttcttt
    11761 ttcatcttca tcatacaaca taaacattcc atccttttat attattcagc tctaccacca
    11821 atttcttcaa gttcaattag ttcttttgaa tttaaaaata tttttaattt ttctgtcatt
    11881 aaattagaat tatcaccttc aagtatggat aatattgctt ctatcaccaa aagtctttca
    11941 cttatttcct gttgatttat agccttaagt tttgatgcta ttggtaacca tattaaattg
    12001 gcagaaccaa caccatacaa agtagccaag aatgcaacag aaatttgagg tcctaatgca
    12061 tctggattac ttgaaagatt acctaaaata tgtactaacc ccataactgt acctattatc
    12121 cccagtgtag gtgcatatcc ccctgctgaa tcaaaaattg ctattccttc tttatgtcgt
    12181 tttgatgtta tctgtatctg tgattccaat atatttctta tagtaactgc atccactcca
    12241 tctaccataa gttcaagccc ttttctaata aaagggtcta attcatcatt ctcacttatt
    12301 tctttgtcaa tactaagaag cccctctttc ctagcctttg ttgcaatttc tttgaaatag
    12361 attatatttt ctatgttatt aggcttttta tttttaaaag ctaataacat tatctttata
    12421 gactttttaa acacagggaa tggagtcgaa gctccaacag ctcctatagt tccaccaaac
    12481 acaacaagtg ctgatgaaaa tacaaataat gctccaacat gtcccccatc aataacaaaa
    12541 gcaactacca aagatgctat ccccactatg aaaaaaatta aagttgtcat ctattccctc
    12601 ctttctttag ttatacatat ctaatcattt atgacattat aaaaagacta tctttaataa
    12661 aaatagcctt taataattgg cggagaagga gggattcgaa ccctcgcacc ggttacccag
    12721 cctaatccct tagcagggga tcctcttgag ccacttgagt acttctccat atctctatac
    12781 tagtaaatta tataataaaa tcatatttta gtcaattgtt ttataaaatt tgagttattt
    12841 tgaattatta aacatctatt tttacttttt tgcttaaatg taagttttaa atatggtaaa
    12901 attatcttgc ataacactta taatattatt tgataaatat acatacatta acaaatatac
    12961 ctaaactctg acttaattgt aagttatgcc aaaactaaaa taatactagt ccaaggagga
    13021 tattttatgt taccacagga aagatatgag aagattttaa gtattctcaa atcagataat
    13081 ataattaaaa taaatgacat agttcaaatg tttgatatat caatagaaac tgcacgaaga
    13141 gatttagccc atatggagag tatcggtctt ttaaaaagag tttatggtgg tgctatacca
    13201 gttatagcaa gtagcgtaga acttaactat gttaaaagaa aaaaaataaa ctcaaacgat
    13261 aaaaaacaga ttgctattaa atgtagtgag tttataaata caaatgatac tatttttatg
    13321 gatactggaa ctactgtact tgaagtttca aaaaatttga aagataaaaa gaatctaact
    13381 gtaataacca actcaatttt agttgcaaac gaacttatga atactaatat aaaagtatat
    13441 gtgcttgggg gctatcttag agaaactgaa ggttcttttt ctggtccgct tacactgtca
    13501 gctattgaac aatttaatgt tgataaagct attttaggcg ctggaggtat cactcttgaa
    13561 aatggaatct ctgactatca cttagaagaa gcacttgtta gaaaaaaaat gattgaaagg
    13621 tctagaaaat ctatagtagt tgtcgaacct cctaagtttg gtataaattc atttgcatca
    13681 gtagctccta tagaacatat agatactatt attacctctg ctagtataga ccataatata
    13741 ataatagagt tttctcaaaa aggaatacct ttagtcatag ctgataatta gcttatatga
    13801 ttgattttta gttccttgat gtaataaatt aaaattaagg agccaaataa ttatttattt
    13861 agctccattt atgtctaaac tatactttat attttaatta tgtaactact ttcagttagc
    13921 ttatcatttt tagttatttg attgccattt tatttaattt ataagtttta gttattcgat
    13981 tattttttaa ttgtctgtct ttttaattta tttttagtta tttaaactcc tactttaaaa
    14041 taatttaaat ttgacttaag tattgtttat tctacactgc atctttcttc attttaatac
    14101 tttgttcttc ttccaaggtt aaatcttttg tcataagtat agcaagtgca attgccaata
    14161 ctcctccaac caattttcca attacaacag gtgtaatcat ctctggaact actcctgctg
    14221 taaatcctaa atggtccccc aaagctgctg tagcacaaac cagccaagca gtatttacta
    14281 ctttcccctt tggactcata tctttcatca ttttatatac tggaatacta ttggctaaag
    14341 taaaaactaa tcctgctgca ctagtggcat ccatacctaa ttttccaccc actattgtaa
    14401 gtggtttatt aagaacttta actaataaat gtaatattgg aaaagtacct aataacacga
    14461 ttgcaatact tccaattact tgaatacctt ctcttatagg tgtcatgcct tttataagta
    14521 ctatacctgt tatttcttga aatgctgctg ctcccaaacc tatcgttatc aatatagata
    14581 taaattttcc aaaaattaat gacccattta tcataagcgt agaattaaat tttaaaccta
    14641 ctgccaatat cactgccaat attatcactg gtatcatatt tataattacc attgataagt
    14701 taaatcctgc tattaatcct cctataatac ctccaaaagg tattgttatc aagcctatca
    14761 gtaacccttt tgcaaagaat ggtctgtctt catattctat aagacctagt cctactggaa
    14821 ttgaaaatac taatgtacat ccaagcatgg atgacacaac caatcctgca agcattcctg
    14881 cctctttatt gtgtgcaagt tccatagcca atggataccc tcccatatca ttcgctaaaa
    14941 tagttgcaaa catagatggg tcagctccac agagattgaa gattggtatt attataggac
    15001 ctagtacatc tgaaattact ggagctaaac aaactatacc aaccattcct aaagccaatg
    15061 gacccattga attaaaccct tcttcaaact gctctccaag tccaaattta tttccaatta
    15121 ttctatctaa cgctcctaaa agcactccca cagccattat gtataatata attttatcaa
    15181 agggcatttt taattcctcc tatacatgta aaatactcta ttgtaactta attagcacac
    15241 acctttttgg tatcgttttc ccaattcata gctcttttta ctgcttgatt ccatccaagt
    15301 aatatttctt ctcttttttg attagtcata tttggaatat attttttgct tattgtaaat
    15361 tcacttctaa tttcatcaat actgttccag acaccacaag aaagacctgc catatatcct
    15421 actccaagta ctgtcgtctc aggattttct gcaatttcaa ttggtatacc taatatgtcg
    15481 gattgaaatt gcattgtaaa gttattttta gtaattccac catctactct caacattttt
    15541 atttttttcc ctgaggctaa gttcatagaa tcaacaacat ctttgacttg aagtgcaaca
    15601 gactcaagag tcgctcttac tatatgctct tttttagtac ttcctgttat cccaaccatt
    15661 gtacctctag catattggtc ccaataaggt gctgctatac ctgcaaatgc tggtacaaaa
    15721 tatacacctc cagtacttga tatggaattt gccatgtcat cagtttctga ataatttttt
    15781 ataactccaa tgccatctct aagccattgt attgcagcac ctgctatata cactccacca
    15841 tcataagcat atgtaacttt atctcctatt ttccaagcaa cagttgtcaa aataccatta
    15901 cctgctatat ttagttcctc acctatattc atatatagga aacatcctgt accataagta
    15961 tttttaacca ttcctggttc aaaacatcca ttgccaaata aagctgctgg ttggtctacc
    16021 attgaagcag taatttttat agaaacccca caaaatgcct ccttatctgt agttccaaaa
    16081 tcaccactcg taggcattat atctgctaaa atacttctag gtattcctat aatgtcaagc
    16141 aactctctat cccaatccat atctcttata ttaaaaagca ttgttcttgc tgctgtagat
    16201 acatcagtta aaaaagattt tcctcctgtc attttccata ttaaccaact atctaatgtt
    16261 cctgcaagta cttctccatt tttaattttt tcttttacac cttttacatt atctattacc
    16321 catttaattt ttgtacctga gaaatatggg tctataatta acccagtctt atctattatc
    16381 ttattttcta aacctggtat attctttaaa ttttccacat aatcataagt tctcctgcac
    16441 tgccacacaa tagcattgta aagtggttta cctgtatttt tatcccataa cattactgtt
    16501 tcaccttgat tatctatacc aattcctttt atatcacttt cattgattcc attttcaaaa
    16561 gcctgttgta atacattatt actcacagtt tttgtattat tccaaatttc tatagggtca
    16621 tgttcaacat atccagattg tggatatatt tgagtatgtt ccatataatc gctaaaaaca
    16681 aactctgatt tttcgtttat caatactgct cttgttcctg ttgttccttg gtcaattgct
    16741 aatacatatt tcactttaaa tgtccccctt tattttcaga attaacaaat tcttttttat
    16801 tttttgaata tataatctat tcaagttatt tatattttat atcatcatgt ggatttatgc
    16861 aatattcatt acaatatttt ttgttatttt taattatagt gattaaaaaa gtatttttat
    16921 tgaaatatta ttgcaatatt tattatttta ttctataatc tagttaaaat aacacataac
    16981 attttaatat ttacacataa tcagtcattc aaaaatttaa atttaacttt ttaaggaggc
    17041 tttacaatga aaaaaacata tctattcagt cctggaccag taatggttac agataaagtc
    17101 agaaattcac ttcttcacta tgatatatgc catagaggaa atgagtttat gaatttattc
    17161 aaagatacac aacaaaaaat caataaacta tataatgcaa cttctaatta ctactcatta
    17221 attgtatctg gctctggaac atctgtaaat gaatgtgtac tatcttctat ctttgacaaa
    17281 gaagatgcag ttctgctagt tagtaatggt gtttttggag aaaggctaga agaaatatta
    17341 tctacttata atgtaaaaac atataaacct aattttgaat gggcagaata cccggaccta
    17401 gacattttag aaaaatacat aattgaaaat tctgatataa aagtaattgc tatggtgttc
    17461 catgaaacta gttcaggaat gataaatccc gtccctgaac taggaaaact tgctaagaag
    17521 tataacaaaa tattttttgt agatgcaata agtgcctcag caggtgaata tattgatgtg
    17581 gaagagttta atatagatat aataacaggt gttggtggca aagctcttgg agcatttcca
    17641 ggttctgcat acctttgtgc taaagagagt attttacaaa atattaaaga aaatcaatgt
    17701 aagaatgtgt atcttaatct ttataaacac tataaagttg ctaaatcatc ttctcaaaca
    17761 ccaaatacac ctaatgttac tttaatattt gcccttaatg aagctttaac tgaatttttg
    17821 gaagatgaca gtaaaataga aagatacaaa gaatgttctg ctattttaag gaacggtatg
    17881 gaagaattgg gtctaacatt tttacttcca gataagtata tgtctaatac agtaacatct
    17941 gtatttctac ctaaagaaat caatgtaaat gagtttatat tagggctaga aagggaaagt
    18001 ggctatgttg tctatccagg aaaaggcaaa ttcctagatg ctaacatatt ccaagtagca
    18061 aatatgggag aaatatatcc agacgattgt tataaattct tagatatctt aaaatcaaaa
    18121 ttggagataa aaaaagttat ataacagttt gtttgtaaaa tcctacaata tatcagtata
    18181 aattaaatag actgattttt ttaaattttc ctataataaa ttatatttta tatttttgta
    18241 taagggtgaa ctataatatg taaagttcac ccctatttca aatctttttc cttcaatata
    18301 atctttttct tttaatttat tttatatttt tgacgcaatc gttttttatt caatcatttt
    18361 atattcttga tacaatcatt cttacactta attatttttt gtttttgaca caatcgtttt
    18421 ttgttgaatc attttatatt cttgatacaa tcattcttac acttaattat tttttgtttt
    18481 tgacacaatc gttttttatt cagtcattta atattttcaa cacaatcatt cttacattct
    18541 attattgttt gtttttgata caactgtttt tattcagtca tttaatattc tcaacacaat
    18601 taccttttca ttcaatcaaa gtaatcttta agcattattt ttcaagctca tcaaagtaat
    18661 tatatacgat aacctcacct gtctctttat ctgtagtctt tataatgccc ttagctccat
    18721 ttacctcttc ttttgtaaat tcatcatcta acacaacttt gaatttatct tcctttccat
    18781 aaccttgttg aaattcatct ctcaaaattg catttttttc tgcttttggc ttcaactcaa
    18841 attcaattcc agatttcatt tcatcaaaat aatttaatac tacaacctca ttattcattt
    18901 tattagttgt ctttataatc cctttagctc catttacttc ttcttttgta aattcatcat
    18961 ccaatgttat attaaatttg tcttcttttc catattcctc ttgaaaatcc ctcttcattt
    19021 cattgtattt agtttcaaac tctttactct tgtttaattc catagctgat acatttgtca
    19081 atgatgctcc aattgccatt actgttaatg ccattactat accactttta aattttttta
    19141 ctttcatatc ttacctccta aattaaatgt tctcaatgaa attatataaa gaaaatccta
    19201 agttttacag tttcattctt gtaactaaaa ttactattat gtaaccaaac tttttgcttc
    19261 aactattcct taaaagttgc ttttttgagg aatagttgaa gttaaataat tagattttat
    19321 ctatatttat agatttaaaa tcaaatttat ccatataaat aaatacttat ttttgcaaaa
    19381 taaaaatttt taattactcc aatccttctt atttacttaa aattctatga taagaaattt
    19441 aaaagtcttt accttcttta acaataattt atttactttt aacatacaaa atagagagag
    19501 tatatcaaaa tcattttact gaatattcta aaaataagta ccccctatta gtatatatat
    19561 ttacatatta aaaaaaagtt ttaaataaag ttatattttt tacaacagtt ttgtaattaa
    19621 aaacatattg aaggtaaatt taaaggtact atatactata aatatgtcga cacaataaat
    19681 ttaaaaagga gaactaaata tgaaaaaaag attaataata atgatattgt ctgttgtctt
    19741 agtactatca agtatactta cgatatttgc atattcaaat attaaatata ataataacaa
    19801 attgatatat tctaatatga tagataaaaa aactcaaaat agtgtaaagg aaatcctaaa
    19861 agaaaataaa attaatgaaa aagacataga tacatttatt aaagcagtca acaattataa
    19921 taaattgcaa gttaaaatac ttcaaaataa tataaatata tctaaatctg gatattcttc
    19981 aatcaatgca aaacaagttc catataacct agaaaaattg caagataatt gggtgaaaaa
    20041 attccctgat tatatggatg taaattgtag aataactgct tttagacttt ttaaagattt
    20101 tataaattca aataagaaat ttactggaga ctctattgat ttgaatgtcg acttggacac
    20161 tataatgaat aataaagatg ctaaatttag caccaaagat gtagaaaaat ttattaactt
    20221 cttttctgca ataccagcta aagacacaga tgatacaatc aaaattgcag aacaaataaa
    20281 aaatgaatgg aaaaaaagaa aaatatcttt taaaaataat aaaaatatat ctattataaa
    20341 tggatttctt cgttatccag aaactaaaaa cgtatttata ggtcatacag gcatatgtat
    20401 taaaactaaa aatggcattc tatttttaga aaaatatggt gttacatcac cttatcaagt
    20461 tactaaattt aaaaataaag aagatgtaaa aaattatatg tttaatagat tga
//
LOCUS       DAGSHT010000052        20139 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGSHT010000052 DAGSHT010000000
VERSION     DAGSHT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.053"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..2245
                     /locus_tag="MJB72_001692"
     CDS             <1..2245
                     /locus_tag="MJB72_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262806.1"
                     /translation="DDRVYQAGTSITIGSSKIKDSSISVLVKEHDKTGLSNYPKLMKL
                     KSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLLNDTIPPYNYTANFKLSG
                     ILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIKQQEKFQEIVNQLAKKIN
                     LSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLFVALLVLLASGLVIYNIL
                     KISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIPIGAVIGIISSKGITGMV
                     TNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLIFSFISALPSAIYASHVS
                     PKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRTIITILSLFMSITVFVAL
                     SEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKMKNVNRTSFIKYSEYKQG
                     EIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFKNGSICFIKNPIAISTPG
                     VKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWINGVDVIVYDSVYNTLTNKN
                     KFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESDKQLKESFKQIEFLAWAV
                     ILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLYKMFLWEGVYYGIFAAIF
                     GSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLACIIATLIPLRKVKKMNII
                     DCIDINL"
     gene            complement(2485..3909)
                     /locus_tag="MJB72_001693"
     CDS             complement(2485..3909)
                     /locus_tag="MJB72_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8262807.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(3928..4620)
                     /locus_tag="MJB72_001694"
     CDS             complement(3928..4620)
                     /locus_tag="MJB72_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8262808.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(4675..5451)
                     /locus_tag="MJB72_001695"
     CDS             complement(4675..5451)
                     /locus_tag="MJB72_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBY8262809.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(5448..6218)
                     /locus_tag="MJB72_001696"
     CDS             complement(5448..6218)
                     /locus_tag="MJB72_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBY8262810.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(6215..6919)
                     /locus_tag="MJB72_001697"
     CDS             complement(6215..6919)
                     /locus_tag="MJB72_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY8262811.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(7174..7686)
                     /locus_tag="MJB72_001698"
     CDS             complement(7174..7686)
                     /locus_tag="MJB72_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262812.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            8012..8269
                     /locus_tag="MJB72_001699"
     CDS             8012..8269
                     /locus_tag="MJB72_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262813.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            8412..9038
                     /locus_tag="MJB72_001700"
     CDS             8412..9038
                     /locus_tag="MJB72_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBY8262814.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIVNG"
     gene            complement(9285..10064)
                     /locus_tag="MJB72_001701"
     CDS             complement(9285..10064)
                     /locus_tag="MJB72_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY8262815.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(10132..10593)
                     /locus_tag="MJB72_001702"
     CDS             complement(10132..10593)
                     /locus_tag="MJB72_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8262816.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(10569..11246)
                     /locus_tag="MJB72_001703"
     CDS             complement(10569..11246)
                     /locus_tag="MJB72_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8262817.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            11478..11864
                     /locus_tag="MJB72_001704"
     CDS             11478..11864
                     /locus_tag="MJB72_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8262818.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENNKLSEDDIEELREILNKRTTKGDK"
     gene            11867..13666
                     /locus_tag="MJB72_001705"
     CDS             11867..13666
                     /locus_tag="MJB72_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBY8262819.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLLSWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGISLFGKTGTANINGKDVNGWFIGYVEKNGNTY
                     FFATNIQSKNYSNGSSASKITLSILNDKKIYNP"
     gene            complement(14021..15895)
                     /locus_tag="MJB72_001706"
     CDS             complement(14021..15895)
                     /locus_tag="MJB72_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8262820.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDKDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(15900..17564)
                     /locus_tag="MJB72_001707"
     CDS             complement(15900..17564)
                     /locus_tag="MJB72_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBY8262821.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDVNLLNKTVRLKPFHAVLLKAR"
     gene            complement(17578..18399)
                     /locus_tag="MJB72_001708"
     CDS             complement(17578..18399)
                     /locus_tag="MJB72_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY8262822.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(18498..19262)
                     /locus_tag="MJB72_001709"
     CDS             complement(18498..19262)
                     /locus_tag="MJB72_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8262823.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(19259..20008)
                     /locus_tag="MJB72_001710"
     CDS             complement(19259..20008)
                     /locus_tag="MJB72_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8262824.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
BASE COUNT         7139 a         3370 c         2377 g         7253 t
ORIGIN      
        1 agatgataga gtttatcaag ctggtacttc aataactatt ggcagtagta aaataaagga
       61 ttctagtatc tctgtacttg taaaagagca tgataaaaca ggactttcta actacccaaa
      121 attaatgaaa ttaaaatctg gacatttgcc taaagataaa aacgaaatag ccttagatga
      181 aaacacacta aagcttatgg gtataaaacc aagactagga gtgactatac ctatgaattt
      241 agatatttca cttttaaatg ataccatacc accatataat tacacagcta actttaaatt
      301 gagtggcatt ttagaggatg attatacagg atatgtaagt ggtatagtaa atggtattgt
      361 aggtaaaggt acatcagaaa atctattacc taagagatat attttatcct ctttggattt
      421 taaaataaaa cagcaagaaa agtttcaaga aatagtaaat caattagcta aaaaaataaa
      481 tctttctcac aattcaattc aatataattg gatttactta aatgctctta agatacagtt
      541 tgaaaaagat gaaaatagtt ctaatagtga tggtatatct atgattatat tggtatcttt
      601 atttgttgcc ttattggttt tgctggcttc tggacttgta atctataata ttttaaaaat
      661 ctctgttaca aagaaaatta aagagtatgg atgtttaaga gcaataggag ctgaaccaaa
      721 ccagatatat aagatagtta ttttacaaat cttaatttta tgtactgttg ccatacctat
      781 tggagctgta attggaatta tatcttctaa aggcataact ggaatggtca ctaatatctt
      841 aaatccagat atacttctag caaatgataa taaagaaatt actgagctta tacataaaaa
      901 tacaacagca tatatgttcc cattagtatt aagcactaat gtatctttga ttttttcatt
      961 tatttcagca ttaccatcag caatatatgc ttcacatgtc tctcccaaaa ttgctatggt
     1021 tggtagtacc accaaaatca aaagaaaaat taaaagagaa aaaacaataa aaaactttga
     1081 aagacatcta gcatggctaa acttaaaaag aaataaagga agaactataa taactatact
     1141 atcattattt atgagtataa cagtattcgt tgcactgagt gagttctcta atgtattaga
     1201 tgtatctagg tcagtaagta atttaaaaga aggagatttt tccttaacaa atgaaatatc
     1261 tggttttgat aaatcaacac tagataaaat agctaaaatg aaaaacgtca atcgcacttc
     1321 ctttataaag tactctgaat acaaacaggg ggaaatagat acagatataa attttgaaaa
     1381 tagtggtgag atgttaaaaa tcattggaat tgatgagcaa actttaaaag acttaatgcc
     1441 ttctattaca gattctatac tagaagactt taaaaatggt tctatctgtt ttataaaaaa
     1501 tccgattgct atttctacac caggtgtaaa aactagacat acaaatttaa aacctaaaga
     1561 tagtatcaca atcaacaaaa aacaactaga tatatatagt acagtagata aaatgttctt
     1621 tttacaaggc aatggctgga taaatggtgt tgatgttatt gtatatgatt ctgtttataa
     1681 cactttgaca aataaaaaca agttcaacca gataaatatt tatgctaaag ataaatcaaa
     1741 attagaacaa ataagattgt ctattgaaca gatttgtgaa aataatcctg gctctcattg
     1801 gatatcatat attgaatcag ataaacaact taaagagagt tttaaacaaa tagaatttct
     1861 agcatgggca gttatattat ttgtgggatt gattggtact ttaaacatca ttaacacaac
     1921 tcatacaaat ataaatacta ggactaatga gattggagtt aaaagagcta ttggtatgag
     1981 taatagtagc ttatataaaa tgtttttatg ggaaggtgtt tattatggaa tttttgctgc
     2041 tatatttggt tcaattgctg ggtatgccag tgctattatt ataaacatgg caactataga
     2101 aaagcttgat ttcactaata taccaattac ttcaatttta caagctacta ttatttctgt
     2161 acttgcatgt attatagcta ctcttattcc gttaagaaaa gttaaaaaga tgaatataat
     2221 tgactgtata gatatcaatt tataactatt ttacagataa taatataaat taaattatca
     2281 atgactgtct ctattttctt tcaaatctaa ctaataaatc tttttaacta taatataagc
     2341 tttaaaaaag gcctgtctca aaatagagat aagccttttt tcattgtcga aataatgaaa
     2401 atatatttaa cttcacaaaa aaagagtgcc tcacgaatta aaaatttcgc tttgagacac
     2461 tcccctttaa tttaaaatat atagttaaat gctatgccaa aatttcacac atgctccacc
     2521 tgattttgaa ttttctattt taacagaacc tccaagctgt tccactaatt gtttgacaat
     2581 gtaaagccca agaccagaat gtccaccttt tgtctgccta gcttcatctc ctcgataaaa
     2641 cttatcaaga gcctttttta aatctttaga gctaaaccct ctaccagaat cacaaatctc
     2701 ataagaaata caattttttt catcctttac agtaatacta atattaccac ctgatggagt
     2761 gtattgcaga ctgttggata tgatattatc aaaaattcgt gtcaacctat ccacgtcgat
     2821 ttgaattggg gattgtatat tcccctgcat tttcaatata agttcaactt ccttttgatg
     2881 agcctgtaat tcatagtctt gcaccttttg cctcaaaaat tcaggtaaat ttataggaac
     2941 aagatttaat tgtatatttg acttttctaa gtcagacgta tattgcatct tttggacaag
     3001 agcagcactt ttttctgtat tttctcttat cactgtaaga tacctatgaa gtttttcatt
     3061 gtcatctgta ttattaccaa taagtgcatc tgtatatccc aaaataatag aaagaggaga
     3121 ttttaaatca tgtgctagag cttccaccat ttcaacacgt tcctgctcca tcttccactg
     3181 tgcagataaa gagcctttta attcgtcttt catttcagaa aaggcactac aaagtttacc
     3241 gagttcatta tctgaataat agtcgatttc aaaatcaagg tccttttctt ttatcttctt
     3301 ggacgcatca atcaatagat gtaatggttg attgatattc tttacaaagc gcttagaaag
     3361 ccatctagta aaacccacga tatatagaaa tggagaaaac agagccatta taataactgc
     3421 aaatacaaat ctccctctaa tatttgcaaa ggtgattttc acttggtaga atagaataac
     3481 agctccctca atctttccat tatccccttt gattggaaca gtatggatat aaccaccttt
     3541 acgaacagtt ttattgataa aattattaaa taattcttct tttgttttaa atagtttttt
     3601 ggggttagta ccatataaaa tattgccatt attatccaca acttgatata gcatatcatc
     3661 cccacgaatc gttcttttta aaccttcttc attcgattgt gataacaaag ttatgttttt
     3721 ttcattgatg tatttttcaa ttctaggaac ttgtcgctca taatagtttg gtggatagat
     3781 atccttattt aaggaatgaa tatacaaata gagagcaaat acataagtaa tcactgtggc
     3841 aactatactg gctattataa taaaaataaa agtatgtcta aattgggtaa taagggattg
     3901 atttttcatt taataacctc tctctcttta cattttattc cacttatacc caatacccca
     3961 aactgtggaa atatattctt tttctggtgt cacagcagaa aacttagctc gtattttttt
     4021 tactcgttca acaacagttg aggaatcacc ttcagaatca cacccccata ctttttcata
     4081 gatttgttct cttgagaaaa cttgtcctgc atggagtgct agtaattcaa caatatcata
     4141 ctctattttt gttaaatcaa tattcttacc atcaatttta attgcacgtt ccttcatata
     4201 taagcataac ttcccaaaat acaaagtaga cctcttgttc tcttcattga tatactgtga
     4261 acgcttttcc ctacgcagat tagcttcaat ccttgccata agttcacgca gtccaaatgg
     4321 ctttgttaca taatcatccc ctccaaaggt caaccctttg attttgtcgg tttctgattg
     4381 ctttgcactt aaaaatataa ttgggcataa aacatcatct ctaattgcct gacatacctc
     4441 aaatccattc attttaggca tcataatatc cagtataata aggtcaggct cctgttttga
     4501 taactctata cctgcctgac catcataagc tatcaacact tcatgtcctt ttgccttcaa
     4561 ttcatcctca agtagcatta ctaggtccat ttcatcatca ataatcaata ttttactcaa
     4621 tataacacca cctttctctt atgggaaggt cagtattaaa acaatattcg tctactaata
     4681 acttttcctt ccctcccaca ttttaaacca aataacgcct cctatcaaga atataatcaa
     4741 acctattgct actactataa gctctatact agctgtttgc atcactttag agtaaaatgc
     4801 ctcagttgaa atagttccag cttctttaat aaaataaatt cctggaagca tacctagttt
     4861 tactggccat gtccatggaa caaatgccca tactttttca ccaacgctag ttaatccaag
     4921 tattgctgcc atcaaaattc caccgatact aataccaata gatgcaccca taccccatgc
     4981 aaaactcacc caaagatgaa tagctaatag tggaagtgtc ccaatcacca caagtcctgc
     5041 tgctgacagg aataaccata ttgttgctcc agatggcact gcaatactca ttcctatgca
     5101 aagtatcaac gtagcaataa atgtgcatat agttaggcaa aacagaagaa aaagaaattt
     5161 tcccagatat aatctaacac gagaaatacc tacacataaa aaaccattaa aatttccagc
     5221 aagctcttcc tcctgcacta taaatcctgc taaaatacct actccaatag gaatgatgaa
     5281 tacagtccat actgtaaaaa atccttcaaa agcaaattct tgtgtacttc cagatttagt
     5341 tgtgagataa aaaaccacac ataatgaaaa aataataggc ataaaaagag taagccactg
     5401 gacagctgtc ctctttgctc gaagccattc tgatgataat agctttatca tttgtcatcc
     5461 ctcctattga accaagctgc agtaagtgct gttaatacta taaacacaac caaagaaaca
     5521 acaatcccaa taggaatcac tgaagtattc aaaagtgggt ctccagcttc taatatagta
     5581 ccgttaggat gtatgttgat gattgggcat accattctaa ctgcccaact ccaaggtact
     5641 gcaatccaaa atggttttgt cgcagcaagg acaccaaata tcatacctaa agctccaact
     5701 cccatactta ggaacatacc tttccatgta gctagccata gctgaagtgg aagtatagca
     5761 agagaaacaa cccagcatac aatacttgcc cctataatct gtccaaaagg aactggacca
     5821 gctttcaata ttaaaccagt tataattgtt acgactatta aaactagcgt tgaaataaaa
     5881 ctgtagactg ccatagctat aactttatta atccaaagtg tcataggaga cacattgtgt
     5941 gtacgtaaag cacgataatt tccagctttt ttttcctgga aagctaccaa agtagcaaac
     6001 aatgcaaatc caataggcag aaataaaaat gaccaccagt taaaaattaa tgctaaaaga
     6061 ctttgccacg cccaagaagt tgtttgagat aatggatttt gcatgagtgt tgattgcatt
     6121 ataaatgcat atgctgcaaa agacactggg aaaaaaacaa taagtttacc cataaatgtt
     6181 cttttatact ttaaaagttc tgattgtaaa gcattcatct gttttgtcct cctttaagaa
     6241 taacatctgt aaaaaattct tctagatttt cattagggtc aggcattcct tgaaataata
     6301 gtcgtcctcc atttataatt cctatattat ctacaacttg agcaacctct gatagaatat
     6361 ggctagaaag aattacagtt ataccttttt ttggaaaaga agcaatcaat tcacgcaatt
     6421 cttgaatgcc aaaggggtca agaccattgg ttggttcatc tagtattaac aactctgggt
     6481 cactcaaaag tgcaatagct attcctaacc tttgtttcat acccatagaa aattgtgaag
     6541 ctctcttttt acctgtattt ttcaaattta ctatttccaa cacttcataa attttatctt
     6601 taggtattcc caatattttt gtatgtacta acaaattttc ctctgctgta aggtttccat
     6661 acaatgcagg ggattctatt aaagaaccta tttttgcaag agattctcgt ttccaaggct
     6721 ttctatcaaa aataatacga ccatttgttg gacgaagaag accaacaagc attttaagtg
     6781 ttgtggactt tcctgctcca tttggtccta aaagtccata tatactattt cgttcaattt
     6841 gtagagaaac attgttaact gccatttgtt ctccatatct ttttgataag ttttttgttt
     6901 caagaattaa atctttcata ttaatttctc ccttcttgta ataccttaaa tctgttacta
     6961 agagaatact aacatgaaaa aataacaatt ctataacacc tactaatttt aataaaattt
     7021 aaaataaaaa atagagttac caattttatt ttggcaactc tatttaagtt tatgttttac
     7081 agttatatta gatttaaact tagtttcatt atcacactaa cttcaaattt actctataat
     7141 ctttaaatca aacactaatt tattaatcaa acactaatct attaatttat atttctttag
     7201 acatctaata tagtttgcta ctaaataata attatttata tcatatttat ctaatccttt
     7261 aaaagaagtt atttcatcat taagctcatc actaccttct acaaaaacac aacctgtttc
     7321 aaattccata cctacaataa tcttacagcc actataaatt tcctcttgag tatcgtacaa
     7381 tttttttgca tttccttcat ttggaattgt aaatacaata ggttttaatt ctttatcact
     7441 ggaaatcttt tgtgaaaaag gcttgtttaa cttaatatta ttgataacta catgtttaaa
     7501 ttcctctgaa gtttcttcta ctattacata ttttcgcttt aaaaactcta ttatttctaa
     7561 aacagactta gaatttactt ttaaaatact tttatatttt tcatataaag atttccactg
     7621 ttcaattttt tcctgtggta tttcttggat atgtgtactt attttttctt gagatatttt
     7681 tttcaattaa actctccttt caatacttaa atattcttat gaatctcaca tgactaaagt
     7741 tacaaggttt ctacttaata gaattttgca tacttaaata tgtcttatta attctctaca
     7801 ggatatctct gtaattccta cgattcttac ataagttact tattttaaat tgttattagt
     7861 tagattctaa aaattattta ttttatcact aatataatac tcttaaaaac tttacaaatc
     7921 aatttaaatt tgctaccttt tatttataac aacttatagt acatttctcc tatgcttttc
     7981 aatatattac ttttaaaagg aggttttcat tatgatttta aatagagctg agcttttccg
     8041 aggatattct ttaaatagat ttaacgagat atgcaatata ttatcagtaa ataaaattaa
     8101 atataagtat aaagtgaaaa ataatgctta ctctaaaaaa ccttttttta atgaaataac
     8161 acttggcaat ttaggtcaaa aggaagattt ctcttatgag tacttaattt atgttcataa
     8221 agacgattat gaacaggcag agtctctaat aaattctaag ttgcgataga ttatttataa
     8281 aacatgacat tctgttgtca tgttttatat gttattatac tttcaaatat gctaatatat
     8341 tttcaaaatt gtgtgcttta aaactagctt tttatagtaa tttaaagctt aaaactaagg
     8401 agactcgtta tatgaaaata aatataaaat caaagttaag tgaatttatt aaacagaaca
     8461 atctttttga tgacagcaga ataatatcct accttcctaa tataacaata gaaacagaca
     8521 aaataaaaac tataatgatt aatgaggttg tcccaactaa caccaatgac gatttttata
     8581 gtgtagataa agatgctgat tatttaaaaa ctactattcc tttatttgac agtgcaggaa
     8641 taaaagtatc taacataaat gatatcctag atatgggtat ctatataact aatgcagtaa
     8701 aacttcctaa atcagaatat acaataactc gtgatacaat taaattacat atgcctattt
     8761 tagaagaaga aattaaatta tttaaaaacc tagaggtagt aatgttaatg ggagatgttg
     8821 caaaaaaatc ttttaatatg attactaaaa agcatataaa aaagaatgtc atcccttcca
     8881 tttcaacata taaacttcgt aataatgaac tttattatga taatttaaga gtatttccat
     8941 catatattat gactggtgga aacatactga ttgaaaaatc aaaatttgaa atggcatcag
     9001 aagatataaa aaaaatgttc gagattgtaa acggttgata ttacaaaata ataaaaacta
     9061 atataaaata aaaagtgcct caaaaacttc tatttatgag gcacttttat tttatattag
     9121 tgtaactata tactattaac ataatttatt atttatattg ttctttttaa tctctattcc
     9181 atttagtcta ggtaacatta ctctttacaa gattacttta tatttctttt atatctctaa
     9241 cgtttttatt taataaaaca attattaata caagtcaaat atatctattt atctaaaaat
     9301 atatctttta cagtaatatc tggatggtga tacttcttcc tattcgttaa aaaagatttt
     9361 tttacattaa tagcctcttt tggacaccag tgaaaacaag ctaggcatct ttcacactca
     9421 ttaccccact ttggcttttt atcaactata cttatattct ctacaggaca aaccctttta
     9481 catgtcccac atccattaca tttattttct gcaaaaaatt tagtagttgc aatttgcata
     9541 ttcttaccta ctaaacgaag tacaacacta tctatatgtg actttaaatt gttttttcct
     9601 tctatatttc caatctccaa gttattgata cattttgcaa tctcagctac cctattagtc
     9661 tgatttttca atctttcttc ttcaacatct ggaggtgtta tcaaagcatt tccaggcata
     9721 ttgattaaaa aaccagcaga taattttttc cccctttttt taagtaactt atctatagta
     9781 aataaactat gtccagctac cccattacaa gtggcaatgg catatatatg agtattaata
     9841 ttaaaattta atttctctac aaaagacttt actatatcag gaacatcagc aaaataaaca
     9901 ggaaaaacaa ttaccaatat atcttcattt atgtctatat tattttcttt tctatgtata
     9961 gctaagggta taacttcttt acttccattt aacgtttctt ttaaaatatt tgaaactact
    10021 agcgaatttc ctgttccaga aaaataataa atcttactat tcattcttat ccctctttct
    10081 atatttctac atcatcaata gttaaaaagt ctcttaaaat aggatttata tttactttcc
    10141 aattctatcc aaaatatcag taaacaaagg caattcttca tcagagatag atttaaatgt
    10201 ctctgttgca tatatacttg caatttcatt taatggttct tctaattctc tacctaaatc
    10261 tgttaggaat atttgaaact tacgtctatc tttaacatct actttacgga caattaatcc
    10321 cttatttttc ataacatcta aaattcttgt tgtattagat ttatccttac atgatttttc
    10381 acataaatct atctgagaaa ttccatcttg ctcccataga aaccttaata acgcataatg
    10441 gtcagttgtt atatcatatc cattttgttt aaacatcttt tttaaatcat cttttacttt
    10501 taacgaagta cttactaact tagttgtagc ttcatatagt ttctcaaaat ctttcatttt
    10561 acctccattt aaataatccc ttgctcctcc catctatcaa ttattccctt atattgctca
    10621 atatctttaa aagccattgg ctttaaaaca gcatttatag caataaaatt atagatttga
    10681 gaaagcacag gtggattttt cttttcctct atgcaataaa taaatttttt agataccttt
    10741 tgtatctttg attctacttt ttcagttata ggattttgat taaatccagt ttttatagat
    10801 aaactaccac aaacataacc tcccgaatct aagaccatgg attttataaa agaaattgct
    10861 tttgaaccac gtgcattttc atatgtagta actgctatac atggcttacg atacaataat
    10921 ctttcaagca tcatatgtgc tctatctgta aacaccttga aaagtccagt tacactactt
    10981 ccataagttg ggctaccaaa tataacacca tcactcttag taattatatc atgaatatac
    11041 tctactttat cattttgatt gatacacttg cccattttat aacaatttaa acaaccaata
    11101 caatatgatg gatttacctc acttaaatca aaataattaa tttttacatc tggtattaat
    11161 ctttctatat ctttatacaa gtaagttaaa acttttgatg tagctccatt ttttctaggg
    11221 cttccattta taactgtaat tatcatttct atatctcctt tttagttgtt atatcaacaa
    11281 taagtatatc aactatattt ttagctgtca atataaacca tttatttaaa gctatactta
    11341 cacagcttaa taaaagtttt atttaactac atgtgcttat ttaatcaact ataaatgaat
    11401 tatttttaat aattgacaaa ctaaattaat gataatatac tttatgtatt acaaatgtaa
    11461 gtagaaggag gtatagtatg aaaaagttac cacatatttc agaagcagaa tatcaagtta
    11521 tgaaaattat ttggaaatat gctcctatca gcactactga agtaattgaa aagttggtag
    11581 aaacaagtac atggagtcct aaaacaattc aaacaatgtt attgagatta gttaaaaaag
    11641 gagctttaac atatgaaaaa aatagcagag tattcgttta tactccactt gttaaagaag
    11701 aagaatacgt agctagagaa agtagttctt ttttaaatcg tttttataat ggtactctta
    11761 attctatggt actaaacttt ttagaaaaca ataaactatc tgaagatgat attgaggaac
    11821 tgagagaaat cttaaataaa aggacgacta agggggataa ataaatatgt atgccccatt
    11881 ctttatacga tttttactca gtacattaac cttatcaata ttaattgttg taattttact
    11941 tgccaaaaaa gtatttaaaa aacatatttc tgcaaagtac caatataata ttggatttat
    12001 attgcttata atgttaataa ttccatttgt ccctcttaaa tatattaatc ttggaaatgt
    12061 atttaactat ttatatactt ttaatcaact tggaagcagt actaatagga cttctatgat
    12121 aaaaaatagt ccaaattctg taattaataa ctcaaatgca ttaaacgatt tttcagtatc
    12181 cctaaaccaa gttaattttg atagtttaaa tattttatta ctactatcat ggataattgg
    12241 aattttaatc atgatagcta ttactattca ttgcaacatt aaaataaaaa atatgaaaaa
    12301 atcaatacaa attttaagag acgaggagat agtatttata tttgaaaagt gtaaaaaaga
    12361 tattggtata aataaggatt ttatactatg caaatcacct attataaata cacctattac
    12421 atttggatac tttaaaccac acattatatt accagataag tctatatcta aattatcttt
    12481 aaaagatata aaatatatct tacttcatga attacaacat tttaaaaata aagatatact
    12541 gataaattat ataatgtgct ttcttcaaat attatattgg tttaatcctc ttgtatggta
    12601 tgcatttaaa gaaatgcgta tagaccgtga aattgcatgt gatatttcag ttttaaaaaa
    12661 gcttaataaa gatagtcata tagaatatgg acaaactcta ataaactttg ttgataatat
    12721 ttcaagacca tcaaatctta cacttacatc agatattggt gggtcaaaaa aacaaataaa
    12781 aaagcgacta ttgaagataa tggattttca taatgaatca aatacgttaa aattcaaaag
    12841 cattttaatt tttacattga tagctttaat tgtatttgga aatattccca aactatctat
    12901 tatggcagat atcaacgaaa aatatagacc tacaaatgaa caaactgtgt acgaggactt
    12961 aagtcagtat tttaaaggtt ttgatggaag ttttgttatg tatgatttaa aatccaagca
    13021 atatcatatt tacaataaag aaaaaagtaa aacaagaatt tctccaaatt ctacttataa
    13081 gatttatagt gccttattgg gattggaaaa tgggataata acaagaaata attcacatat
    13141 cccttgggat ggaaagcatc attctaatag ttcttggaat gaagaccaag acttattttc
    13201 tgctatggaa aactccgtaa actggtactt tcagactcta gataaaaaaa caggtctaaa
    13261 aaacatacaa acttatctta ggcaaattgg atatggcaat tatgatgttt caggtggtat
    13321 ctcaaacttt tggctagaat cttcattaaa aatatcacct attgaacagg tagaattact
    13381 tgaaagtttt tatacaaata agtttaaatt tgaagataaa aatatagaaa cagtaaaaga
    13441 ttcattgtta atatcaaaaa aggatgggat atcacttttt ggtaaaacag gcactgctaa
    13501 tataaatgga aaagatgtaa atggttggtt tataggttat gtggaaaaaa atggaaatac
    13561 ttatttcttt gctacaaata ttcaaagcaa aaattattct aatggtagtt cagcatctaa
    13621 gataacatta tccatattaa atgacaaaaa aatctataat ccttaatata tttactgtac
    13681 attctaaaaa attcacataa ttttcatatt aattatcgat taaattcata tacaaggctt
    13741 attatataaa cataaaagat agtaaaacta gcttaaaaac ataatataat accctataca
    13801 aatagtggca actctccctc acatcctcaa ttgttgccac tatttaattt tttgtatgaa
    13861 gttaaatata ttttcatcat ttcaacaatt tgggtgcaat acaatctcta ttttgaaaca
    13921 acctctttaa ttgttttaga gtttatgaat tatttctcat actttaaaaa gattgaatta
    13981 ctcttgataa tcttcttttg tagtcattac atatcaccta ctactcaaca catattcttt
    14041 gacctatttt tacatccttg tttatatcca aaatatttac cttcttacca tctggaaaca
    14101 ataccattgt caaagtagac tttcctaatt catgtattct ctttaaatca acattaacaa
    14161 gaacatctcc ctgtttaacc atatctcctt gtttaacagc aacatcaaag ccatctccat
    14221 ttagtgcaac tgtatcaatt ccaacatgaa ttaatacttc tccattagtt cctttaatcc
    14281 caaatgcatg tccacttgaa aatactgttt ctaatttacc tgaaatagga gaaacaattt
    14341 ttccatctat aatatcaata gcaaaaccat cacctaacat ttttttagaa aatgtttcat
    14401 ctggtacatt ttctaatgca acaattttcc ccttagctgg agacacaaag ttttcattat
    14461 tttcattaaa cactaattct tcgtctttat cttcatttat agcttcttta ggcactccaa
    14521 ataaacatgt tacaataaag cagaatgcaa ccacaaatat tgatactaat aagtatgtga
    14581 ttaaattata tccatcatag atatacatta aataagaagg taaaactgct aatccatatg
    14641 aattagattg aatatgtaaa attgataata ttgctgctcc taaacctgaa gatgcacaca
    14701 taataatcaa aggctttaaa ttatatcgaa gctgaatccc aaataaaaca ggctcactta
    14761 taccaaaaag ttgagatagc attgctccaa ccgcagtttg tttaacttga ttatttttag
    14821 attttttcat aaatgctaga catgttccaa tattcgcaaa tccatacatt gcacaaagag
    14881 taataagggc attaaatcct gtatttgcta atagtgatgt ttcaatcatt acataagtat
    14941 gatgtaagcc agtaataaca gctaatggat atgtaaatcc aataataaat cctccaattc
    15001 ctaaaggcaa atcaatcaat aagctgatga tatcaaccat ttttaattct attgtatgca
    15061 ttatagggcc tacacctaac aaaataacta acattgttat tagcataaca aagaatggtg
    15121 tgaaaataag gtctaataca ttaggcatat gtttacggaa ccatttttca agattagctc
    15181 caataaatgc tgtaacaata gctgttaaaa cacttccttg acaacctaca ataggaataa
    15241 atccaaatgc cataattgct tcaatttcat ttgatgggtc agcaacagaa tatgcattag
    15301 gaagtattgg ggaaactaac ataagtccaa taacaagtcc aataacaggt gttcctccaa
    15361 atactttaaa tgctgaccat acaataattg ctggtaaaaa agcaaatgct gtttctgtaa
    15421 gaactgaaac aagtgtctga atatacaatg gaatatttgc agaagacatt ccaaataatc
    15481 caagtacatt atcattaaac aaacaaccct ttaatcccaa gaataatcct gttgctgcaa
    15541 taactggaat aataggaaca aatatatctg ctagagttct cataactttc ttaaatccag
    15601 tttcattatt tttgactaat tcatcttgtt ctttttttga taaagttttt aatcccattt
    15661 tttcaacttc tgcataaact ttattaacta ttcctgttcc taaaataatt tgatactgtc
    15721 cagaagtaaa gaaaacccct ttaacctcat caactttttc aactttctta tcattaataa
    15781 tttctctatc tttaaccatc actcttaatc ttgtggcaca atgtgtaata gaaataatat
    15841 tatcttcacc aataatttta ataagctcat ttgctatttt attatattta ttcatttttc
    15901 tacctcgctt tcaataatac tgcatgaaat ggtttcaaac gcactgtttt atttaataga
    15961 tttacatcat ctaaattttg gaatacaata tcatattttt cttctactct tatttctaca
    16021 ggtttatcag aaaaattata ataacaatca ataactacca aatctaattg ccttctatac
    16081 gcaataacat cttcactttc tatatcaata ggttctattg aaccataaat caaacaatca
    16141 gaatactcac tatcttgtct caactttatc atctttttat aaaattctaa aatactctct
    16201 gaattgttaa tctgactctg tgcattaata tctttataat tttcaataac ctttaaccat
    16261 ggtgttgtat ctgaaaaacc tgcattttct ttatctgccc atggaaatgg cgttcttgca
    16321 ttatctcggc ttcttaaatt cacaaattct aacgcttctt cttctgagta accttcatct
    16381 attgaacgat agtattggtc aatgctagat aaatcattaa aatcatttat ggtatctctt
    16441 ttaaaatttg tcattcctaa ttcctgcccc tgataaataa atggggttcc tcttaaaaag
    16501 aaatacattg ctcccaacat ttttactgca tcatcattcc tttcgtattg caaaagatac
    16561 tttgttgtag ctcttggttg gtcatgattt tcaataaaat tagctgccca tccatgttcc
    16621 tgaacataca tttgactatc taaaatgcat tttcttaaat ctggaattgt ccatgaaatt
    16681 cttttaaacc actcactacc agaagcaaca tctaagtcag catgtttaaa gtcaaagacc
    16741 attgaaaaaa aaccatcttc tccaataaaa tcacctaact ggtcatagct aatacctgct
    16801 gcttcagcaa cagtcataca atcatatttt ttaaacgtat tttcttttaa ttcataaagc
    16861 attttttcta ttccaggctg atttctaact gtttttgtac atttaacaag accatcaaca
    16921 ccatcacttt ctaaactttg aaaacttaaa ttctttttga taaatgtaat agcatcaact
    16981 ctaaatcctg ccactcccat ttgagaccag gtattaatca tttggtaaag ttcacttctt
    17041 aatattggat tttcccaatt aaggtcaggt tgttttttac caaaagaatg aaaataatat
    17101 tcgtctctat tctctacctt ttcccaaaca cttccaccaa aaacagacct ccagttattt
    17161 ggtaaatcgt ttcctttttt aaaaatataa taatcatgat aaggactttc tggatttttt
    17221 aatgcttctt gaaaccaagg atgttcatca cttgtatgat taagaactaa atctaatata
    17281 attttgattc ctctatcttt actttcttta attaactctt ttaaatcttc catacttcca
    17341 aattcacttg caatatccaa ataatcacta atatcatacc cattatcgtc cataggtgat
    17401 ttaaaaattg gacataacca caatgatgta atacctaaat cttttaaata atctaaacga
    17461 gaaataattc cctgtaaatc tccaattcca tcattattac tatcttgaaa gcttcttgga
    17521 taaatttgat aaacgatttc tttttgccac catttctttt ccataaatta ctccttttta
    17581 tatgatattt cttcttttta attctttata aattccatta tcgaatatgt cctcacatat
    17641 agatgtcgct atatctttaa gttgttgatg actatttttc attgcaattg taacgtctga
    17701 tgcttgaaac atgacaatgt cattttgacc atcaccaaaa catatggttt ctttttgagt
    17761 tactcctaat ctttcttgta atctcttaat tgctttgccc ttatgaaaat ctttttgaat
    17821 aatttcataa tactgactag aaatatctct ctgtgctaat tccattttat cttgtagtat
    17881 atctttcact tcgtcaaata ccttttcatt tgaccataaa caaattttat gtatatcttg
    17941 tgacttatac tcttcaatgt tattttcata tgttattttt tcttggatat gttgtttatt
    18001 tatacaagaa ttagttcctt tcaactgatt cattgtttca aatatttctt tggctttttg
    18061 attcatgaaa actttttcct gactctcaat cgaaaaagca acttctctct tttttaataa
    18121 acaaacaact tcctttatca atctttgatt aaaagattga ttataaagta attcaccatg
    18181 atattgaatg taattacctc ctcctgcaat atatccatca actcctaatg ataaaacatc
    18241 atcttgtatt gtacccatac ttcttccagt acaaataaca acactgcaat gattcttttg
    18301 acataaacgt atagcatgct ttgcagattc tgggattcca tatacctcat ctctaagcgt
    18361 accatctata tcaagtatta ataatttgta caatttcatc ttcctttctt actatcaaaa
    18421 tcataatagc attcaaaaaa aaagatatca atattttgat atcttttaaa aaatatgttc
    18481 atttttagca caaattttca ttttcgggga atatgttcac tttctaaata catcttgtat
    18541 ttaatataaa gcagttcaat taaaataaaa taaggtgttg ttacatcgta gtccagttgt
    18601 ttatctacag aaatagatat agattgaata tacaggtttt catcacataa tcttgctaaa
    18661 ggattactta tcatttttgt aatagaaacc atataaattc ctttcattct catttcttta
    18721 gcaaaatcaa taattgtaga tgcatctcca ttcaatgaaa taaagaaaat catatcatct
    18781 gtttttataa aatgattaag tgcctgaatc atatcataac cataaacatc atatataatt
    18841 ttccctgttg gtaagaaaat ccttttcatc tctttagcaa ctctttcctg agaataacct
    18901 tctccaaaga cgattatacg atttgccttt ttcattcttt caaacataat cgaacaatcc
    18961 ttcttctcta tatcatctat caccttatta taacaatcca ttaattggtg tttactttga
    19021 tttttatatt ctacttctgt ttcatttaag cgtataataa ctcttaattc actatatcca
    19081 ggtaattgta gtttttgagc aaaacgagaa agtgcacttg tagatatatg gtatttgtct
    19141 gcaaattcat ctatagaaag tttaatacaa tcttctttat gattaagaat acactcacag
    19201 atatacttat catttgctgt aaatgaatta taatatttat tgaaaagtcc ttcaattttc
    19261 attcttttct ccttctatac tctatgtaat tcatatgaag caaatctaat agagcataaa
    19321 atgctcctac gacttcataa gatatagctt gtaatgtatc aagtttttgc gtttcaacat
    19381 acagattata ctcacaaagt gttgcaattg tattattatc aagcctagtt aaagaaatcg
    19441 tatgtatatg attgttgata aattttgcaa tttcaattcc tgcttttgtt tctccagaca
    19501 aagaaatgat aataaataaa tcactttttt gaaatgattc tctcataaat tcaatttcac
    19561 ctatatcaaa taagtcaata acacatttac ctactgaaag aaatattctc tttatttctt
    19621 gagctaaact tttctgttca ttacctgtcc cataaatata aattgtttct gcatcatata
    19681 ttattttaca aatatcttga aatgatattt tttgtaattc attcattaat agtcgataat
    19741 tctcgcaaac atcatgaaaa ttattatcat tattaacaaa ttctagttga ttatttttaa
    19801 gtaaatactt taaatcagag aatgaatcta atccaatttt tctacaaagc cttaataaag
    19861 tagctctaga aacattacat tcatttgcaa ttttttcaca cgaaaaatta cggtattctc
    19921 tttgacaaat caatatttgt ttaataataa ttttatcatt ttcatttaaa ttatcataat
    19981 tttggttaac taacgattct aatctcattt tttccactcc ctaaatatta ttttaatata
    20041 cattagttgc tttttctagg gaagagtctt aaataataag gataaaataa tcatttgatt
    20101 ttatttttat taaaataaaa ataacctgct aaaagcagg
//
LOCUS       DAGSHT010000053        19730 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGSHT010000053 DAGSHT010000000
VERSION     DAGSHT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.054"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1548
                     /locus_tag="MJB72_001711"
     CDS             <1..1548
                     /locus_tag="MJB72_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8262825.1"
                     /translation="LQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENILEINSISEE
                     VSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKISYFSNCKEL
                     LCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIKLESKHEFK
                     KNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFPVFEGIREE
                     EIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPTIEIVGIVS
                     IKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKENEKYFNLE
                     LIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNIIKSVRDWK
                     EAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKEGVNKLAMS
                     LLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLIINGDKSSIL
                     ELINKQN"
     gene            1561..1848
                     /locus_tag="MJB72_001712"
     CDS             1561..1848
                     /locus_tag="MJB72_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8262826.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            1981..3240
                     /locus_tag="MJB72_001713"
     CDS             1981..3240
                     /locus_tag="MJB72_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBY8262827.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            3243..3983
                     /locus_tag="MJB72_001714"
     CDS             3243..3983
                     /locus_tag="MJB72_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY8262828.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            4306..5616
                     /locus_tag="MJB72_001715"
     CDS             4306..5616
                     /locus_tag="MJB72_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY8262829.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            5714..6631
                     /locus_tag="MJB72_001716"
     CDS             5714..6631
                     /locus_tag="MJB72_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBY8262830.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            6647..7456
                     /locus_tag="MJB72_001717"
     CDS             6647..7456
                     /locus_tag="MJB72_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBY8262831.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            7514..9262
                     /locus_tag="MJB72_001718"
     CDS             7514..9262
                     /locus_tag="MJB72_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBY8262832.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            9524..10546
                     /locus_tag="MJB72_001719"
     CDS             9524..10546
                     /locus_tag="MJB72_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBY8262833.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEVSRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            10551..12857
                     /locus_tag="MJB72_001720"
     CDS             10551..12857
                     /locus_tag="MJB72_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY8262834.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            12829..13944
                     /locus_tag="MJB72_001721"
     CDS             12829..13944
                     /locus_tag="MJB72_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262835.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            13962..15380
                     /locus_tag="MJB72_001722"
     CDS             13962..15380
                     /locus_tag="MJB72_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBY8262836.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            15395..16603
                     /locus_tag="MJB72_001723"
     CDS             15395..16603
                     /locus_tag="MJB72_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262837.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(16677..18065)
                     /locus_tag="MJB72_001724"
     CDS             complement(16677..18065)
                     /locus_tag="MJB72_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBY8262838.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            18406..>19730
                     /locus_tag="MJB72_001725"
     CDS             18406..>19730
                     /locus_tag="MJB72_001725"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBY8262839.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNV"
BASE COUNT         7602 a         2108 c         3500 g         6520 t
ORIGIN      
        1 cttcaaacaa tggggttaag attagagtat aagggaaagt acaaaattat aggtgatgaa
       61 tacgttatta gagagctgtt tctgataatt gttcaagaaa atatattgga aattaattct
      121 ataagtgagg aagtaagtag tttcgataca aaaggacata taaaacttac tgattattct
      181 ttattaaatc taactaagtt tgtggaattt ctaaataaaa ggataagaga tgggaaaaca
      241 ttatactcat ataaatatct aaatgaagct aaaaaaatta gttactttag taattgtaaa
      301 gaacttttat gtgaagaagc taatgaaaat gaacaagcct atatatgcac ttacataagt
      361 tcacttccca gcttaaatag tgaagttaag gaagatgttg ttgaagaata tgtagataaa
      421 cttatagata agtttgaagt aaatacagca ataaaattag aaagtaagca tgagtttaag
      481 aaaaatattt taaggcacct ccattcttct tacaacagaa tacgatttaa gtttcctatt
      541 agaaatccaa tgttagatga gactaagtat aaacatgagt cactttataa aattataaaa
      601 tcaattatag aaaatgaaga agagtttcct gtatttgagg ggataagaga agaagaaata
      661 ggatttatag cagcgtattt tggaggatat ttaagagggt ctagggataa tggtctaaga
      721 agaaataaag tcttactagt ttgtcctaat ggtcttatgg tatcaaagag tttggaaata
      781 caactatata aatacatacc aaccatagaa atagtaggta tagtatccat taagcaacta
      841 aaagaagtaa atgtatacta cgattacata attacaacta tagatataca aaatgtaaac
      901 aacgtaattg ttgtaaatcc tcttttaaca tcatcagatg ttcaattact tatgaacaaa
      961 ctaataagtg taaaagaaaa cgagaagtat ttcaatcttg aattaataat acaagctata
     1021 agaaaaaatg gagttatcaa taatgaagaa gcactaaaag ctgatttatt aaacataata
     1081 cacaagatag atgaaggaga gatgtatcaa ccaatgttaa aggaattaat taatgcagaa
     1141 agggtcaata taataaaaag tgttagagat tggaaagaag caataaaaat agcctcaaag
     1201 ccattgttag aagataactc tatagaagag ttatatatag aaaatatgat aaagtcagtt
     1261 gagaaatatg gaccatatat tgttttagct gatagatttg ctctaccaca tgcaagttct
     1321 aaagaaggag taaacaagct tgctatgtct ttactaatag tagaagatga agttgattta
     1381 ttaggaaaac cagtaaatat atttatggta ttagctgctg tagataacac tactcacatt
     1441 agagcattag catcactatc agagatgatg tacgaggaag aaaatgtaaa actaataatt
     1501 aatggtgata aaagttcaat actagaactt ataaataagc aaaattagga ggaatgaaaa
     1561 atgaagatat tagcagtatg tggttttggt gtaggttcat caatggtatt aaaaatgaca
     1621 atacaaaagg ctctaaagca gttaggtata gaatgtgaag tagaaaatac agatataaat
     1681 tcagctagag gtgctgattg tgactgtata ttcacaagtc aagaattgtt aaatgaatta
     1741 aaaggaactt gtaatgtaga tgtatatcca gttaaaaagt acatggattt agcagaagtc
     1801 acagaagttg ttcaaacttt tttaagtgat aaaaatgata agaaatagta taagttttaa
     1861 aaagtaaatt taagttaatc agaataaaat aaaaacttac taaattcaaa aatagtaaga
     1921 aagagaggag ggatttagtg gtagaaaact taaacagttg acctatcact aaaaaatata
     1981 atgcttgatt ttatttcaaa taatatttta cgtaatccat ctatgttaat tggattaata
     2041 gcagcatttg gtcttgtact acagaagaag aaagcatctg atgttataaa ggggtcacta
     2101 cttgcagctt tcggaattat aattatggag actggaacag gtatgttagt tagttcaata
     2161 gcaccaatta atggagcttt ccaaagtata agtggtggtg cagtaacaaa aggtttaagt
     2221 gatgttactt ttactgcaag ttatggtgga attataggtt tagcaatgtt tgcgggttta
     2281 gctattcacc taatgatagc aagatttact cctattaaga caatcttttt aacaggtcac
     2341 atgctttatt ggttcccatt catatttgta gcttcaggag ttgaagctgg attaaatggt
     2401 ggagtattaa tagcttttgc agcagtatta tcagcactct attggtcaat aatgccttgg
     2461 ttattaagaa aatatgtgtt tgatgttata ggagatgatt cttttacatt aggtcatcca
     2521 tctggttgtt tagcacttat atcaggattt gttgctaaat tagtaggaaa taaagaaaaa
     2581 tctacagaag atataaacat acctcaaagt ttatcatttt ttagagaaat atctataagt
     2641 ggtggaatgg cagtgttttt aatgtttata gttgttggga tagtaatacc aggtgcgttt
     2701 aaagatagta atgagtttat tggaacgtat gcaataaagc aaggtctttc ttttggagca
     2761 ggtcttataa taatgcttca tggggtaaga atgctaataa atcaaataat gccagcattt
     2821 caaggtatat ctgaaaaagt tgtgccaaat gcactacctg cttttgactg tccaatatta
     2881 tttaattata aaccaaatgc tgtattaatg ggattcttag ttgctatgct aacatctaca
     2941 gctatgattt taatatgtaa ctactttaat gtatttaatt tgctacttat accacttgtt
     3001 ataacttcat tctttgagtg tggagcagca gcagtaatag gagaatcaca aggtggtata
     3061 cgtggagcta taataggtac agctatagct gcaatattta tggtagtttt agtaggaata
     3121 tctgctgtaa tttactcagg aactattcaa aattggatac taatatttgg aggtaatgac
     3181 ctttcattat ttggtataat cagtaggttt ataggttcac tatttggagg aatattatag
     3241 atatgaaatt taaatacata gatgagataa gaagattaat tgatataata gaaaacgaag
     3301 aaaaagtcaa tatggaaaaa gcagtacaaa tctttgttga agcaatagaa agtaaaaatg
     3361 ctatattttc atttggagct tcacatgcag gaatattaac agaagaatta ttctatagag
     3421 caggtggctg tgttcttata aatccagtat ttgcaaaatc tctaatgtta gatacagcgc
     3481 ctataactca cacaagtaag atggaaagat tagaaggata tggtacagct ctagctgata
     3541 aaactccaat taaaaaagga gatgtagtta tagttcattc agtatctggt agaaatccag
     3601 taagtataga gtttgctatg aaagctaagg aaaataaggc aatagtaata tgtataacta
     3661 atttaagtta ctcaaaaagt gtatcttcaa gacattcttc aggaaaaaaa ctttatgaag
     3721 tatgcgattt agtgatagat aatcatggtc aaaagggaga tgcctgtgta gagatagatg
     3781 gattaaacca aaaagttagt ccaacttcta ctgtaatagc aactactgtg atgaactcta
     3841 ttatagctca agctactcag gaattagtta ataaagggct aaagaatcct ccgattttct
     3901 atagtgcaaa tattgatggt ggagatgagt taaataagaa gatttttgat gaatataaaa
     3961 gtgtaattca ttatgaatat taaggttaaa aaaatatgtt aatgaacttt gaagtttatc
     4021 aacaaagaat attaataata tataataaat gctatcattt aaaagctgta ggtaaaaatc
     4081 tacagctttt tattattgta gattaaaaag atgcgtaatt aaattttaaa ttggagaaaa
     4141 taaattggaa ggtatcaatt ataaatatta gaaacgttgt catagaaaaa aactatatta
     4201 aaaaaaagta catagaaaaa actgattaga caataaatac atgctttgat aatattttaa
     4261 atggagtgaa aatgtgtaga attataatga aaaaagaaaa taaaaatgaa aaaagaaaat
     4321 aaaatggagg gaataaaagt gggtatgtta aaaaaattat gttcactagc tatggtagca
     4381 attatgacta caacacttat aacagggtgt tcaagtggaa atggaaaaga ttcaaaaaag
     4441 gatggcgaaa aagaaaaact agttctttgg atgccaccag tagaaaataa tatgaaggaa
     4501 gtttgggacc ctattttaga taaatttgag gaaaaaaata actgtgaagt agatttacag
     4561 ataattcctt gggataatta ttctgaaaaa tttgcaacag ctataagtgc tggcgaaggt
     4621 ccagatgtag gatatatgta tgcagaaatg tatccacagt ttatagaaag tggagcagta
     4681 gaagatttga ctaactatgc aactaaagag gataaagagc aatctattta tataaaaaca
     4741 tctgaaatga tgggtggaat gtatggtatg ccattccaag cagcaaatcc aggggtatta
     4801 tactataaca aggatatttt agagaaacta ggagaaaaac ctcctaaaac atgggaagat
     4861 tttaaaagaa tttgtaaaaa agcaacgcaa gatactgatg gtgatggaaa gatagaccaa
     4921 tggggattag ctcagggatg gggagctaaa acttttggta acttaaactg gaactggtat
     4981 ccttatttat ggcaagctgg tggagatata tttaatgatg atttaaaatc ggttaaattt
     5041 aatgataaga gtggtttaga agcagctaag ttcttaaaag aattacaagc atatgtgcct
     5101 gaagattctt tatctaaaga tagtaatgaa atgatagaaa gtgtatttgg accagggaaa
     5161 gcagcattta caataatgtt atcttctgct gcaacaagtg tatttgatga atcattcccg
     5221 gatttaaatt ggggatttgt aactggacta gaagataaga aagcagcaac ttttggagca
     5281 gtagaccatt tatctttaat ctcatctgcg aaaaataaag aacttgcata caagttaata
     5341 caacatatgt taagtgttga atcaatgaca gaatttcata aagcaatacc aagagcacca
     5401 ataacaaaag gtgaaccata tcaaggtgat gaaagattca aagaaatggt ggaaaatgac
     5461 aaaaatgtat atagaccatt agtagtagga cctcatggag ttgaaatata tgaatattta
     5521 tggaaagaac ttcaaactat gatttcagga gataaaactc caaaacaagc gttagatgat
     5581 gctgcaaaat attcaaatga tttattagca caataaaagt ttaaagctgt aatatgttgt
     5641 aaagtacttt acagattaaa attagcattg tatttataaa tacaatgcta atatttatat
     5701 tggagaggat aaaatggaaa acttagttag agagtttgaa gaaaattcaa aaagaaaaaa
     5761 aacaaaatta aatattaaac catatatgta tatattgcca ttgggaataa ttttagtggc
     5821 gttttatgtg ataccaataa taatgtcaat atactttagt tttacaaagt ataatattat
     5881 tagtccagct acttttatag gtttagaaaa ttataaaaaa ttatttacag atgaaacatt
     5941 aaaagtatct attataaata ctattaaatt tactgttgtt gtagtacctt gccaaactat
     6001 attatcattg attttggctg tttggattac aggaaaagga aatagtaaaa tagcttcatt
     6061 tgctaaaggt gctatattta taccagtatt atcatctatg gttttgatag gtatggtatg
     6121 gagggcttta ttgaatggag aaggttctat tatatatcag gtattaggtg cctttggaat
     6181 agaatcatct aaacttttag gagatagtaa gacagcatta cctacactta tgtttatatc
     6241 tatgtggaaa aatatagggt atttcatggt aatttatatt tcagctataa tgaatttacc
     6301 aaaacactgc tatgaagtag cgaaggttga tggggctaca aaacttcaag aatttataaa
     6361 aataactgta cctttattga aaccaactac aataatggtt gtatttttag ggtcgatatg
     6421 gtcattacaa gtatttgact taatttatac tgtgacaggt ggaggtccag gtatatctac
     6481 tatgagtata gttatgcatg catttaactt aaactttaaa aactttaatt ctggatatgc
     6541 aatgactgta gcaaacgtat tatttttgtt aatagcagta gtttcgattt tacaaaataa
     6601 gcttgtaaaa cgagacaatt cagattttta gaaaggtagg tataatatga aaacagcaag
     6661 aagattatta agtgatggtt tacttttatt tatagcgtgt gcaagtttgg taccttttat
     6721 atacatgttg ataatatcat tgaagataac atataactca tatagtttgg atatatcttt
     6781 ttcaacagta acccttcaaa actatataga tatttttact aaaaaaggat ttgcacaata
     6841 tttttttaat acagcaatag tatctttttc aggtgtatta ttaaatttag tatttagtac
     6901 attagcagga tattcttttg cgaagatgga ttttaaaggt agtgataagt tatttttatt
     6961 tatgataatg actttaatta taccttccca agttacaatg ataccactat atataataat
     7021 gaaacactta ggatggataa atagttatct tgcacttatt atgccaatac caactgcatt
     7081 tggcgtattt ataatgagac aagctatttt aggagtacca aaagagttat tagaatcagc
     7141 aaagatagat ggttgttcgg attttagaat tttaatacaa atagtgttgc ctttaattaa
     7201 accagcatta attacactag caatatttac ttttatggga gcatggaacg agtttatgtg
     7261 gcctcttata gctacaacta aagatgcaat gagaacatta acagttggat tatctactct
     7321 taagacattc cagataacta attatggaca gatgatggca ggggctacaa taacattttt
     7381 acctccattt attttctatt tgattttaca gagtaaattt gttgaaggtg tgtctttgtc
     7441 tggtataaaa ggttaaatag tctaatttaa taatatataa agaaacttat ataaatatgg
     7501 aggaatttaa actatggaaa tttcatatgt actgaaagat tgcacattca aacacaataa
     7561 atactcagtt gtagaagctg aaacttggaa agataaggat ttggatattg tagtagctaa
     7621 aggtgaaaaa tttgcatttc aaattatgtt aaaagcaaca gaagaattta attgtacaat
     7681 tgataaaagt agtgctatat catggaaagg cctgggaaat agagtaaggt tagcattaaa
     7741 tatgcctaat agtttagaaa ataattttag tataaatata ttaggatatg ttcaagatga
     7801 tacaaaggca tttgtgaatg atgctatttt aagggataaa gatgtgttag tagagcaata
     7861 tttgcctcaa acattttgga ttgaaggtca agtaccagaa gattttgaag aaaataacct
     7921 tgatataggt attgatatat ttaaaagctt tggatatgaa gatgaagaaa aagtttgtac
     7981 aattgatgtt cctgtaaaag taagaaatgt tgtgctaaag cctttagatg aaagcaagtt
     8041 ctttttagat ttatggcaac atccaagctg tttagctaga atgtataaag ttgagttatg
     8101 gtctgatata cattttgaaa tagtcgataa ctatttaaaa gaattagcat ctttaggaga
     8161 aaaagttgca acagttatag tttcagatta tccatgggca gggcaaagtt gttataaagt
     8221 ttataaaaat ccatcaaact tatatgaata taacatggta agtgtatcaa aaggattgga
     8281 tggaaagatt aaatgtaact ttgagagtat ggataggtat ataggcatag ctgataagta
     8341 taaaatggct aaggagatag atttatttgg tctattaggt aattggtgtg ctggtgagtt
     8401 tggaaatcct gtagaaggtt ataaggaccc tataagagta agatattttg atgaaaagga
     8461 taaagtattt aaatttataa acaatacaaa tgatttaaaa gaatatatag gattagtatt
     8521 aaatcattta atagagtgtg gattatggga cagagtaagg attatagctg atgaaccaaa
     8581 taatccagaa gtagtcaaag agtgtataga atttataaat tctactgtag gtactcatca
     8641 agttaaatat aaatcagcaa ctcacgacca aaacttttta gacagagcta aagatgaaat
     8701 tgatgatatg tcaataaatt taaaacttac tatacaaaat tataaagata tagaaagtct
     8761 taagaaaaaa atcaatgaca agggtgggat attgacttgg tttgtatgct gttttccaga
     8821 aaaaccaaat agttttttaa gttctccttt tgtagaaaat agaataatag gatggtatac
     8881 ttattacttt ggtcttgatg gatttttaag atgggactat aatttatgga cagaagaccc
     8941 ttggaaagat tcaagttaca aattccctat ttggaaagct ggagatatgt tctttgttta
     9001 tcctggaaaa gatttaaagc cagttagaag tgtcagaatg gagaatttaa gatttggaat
     9061 acaggatttt gagttattta caatgttaga aaaagaaaag gtaagagagt atatagaagt
     9121 tgagcttatg caagaattat taaataaaaa agaaaatgct gaaattaaag gttttggtga
     9181 catagaacta ggatattcac ttgataattg gagatatgat aaagttaaaa aatatgtatt
     9241 agagttgtta gacagagtat aaaaaatata agagtgtctc aaaatgaact ttttagttca
     9301 tgaggcactc tttttgtatg aaatcaatat agtttcattg tttcaacaat gaaaaatagg
     9361 attatttcta ttctaagaca gccctttagt tttttagttg tattcaatgt tttaaaacta
     9421 actataaacg ctataaagtt tataaatgac aataattata tataacatga tgtactttaa
     9481 tttgctataa tagggattga ataatattat atggaagata acattgtttg gggggattaa
     9541 tataaaaaga ataggtatta ttataatgtt ttttttaagt atatctttta ttggatttta
     9601 tcttgtaata agagataaca gtgatacagt taagatgcaa cgagggataa atataggtaa
     9661 tgcactagaa tcacctaagg attttccgtg ggatgtaaaa atgtcaaata agttttttga
     9721 tgatataaaa gatgcaggtt ttgacacagt acgtatacca gtaaggtttt ctgattatac
     9781 gtcagattct gataatttta aaattgatga agaatttttt aaaaagattg ataaatatgt
     9841 agattatgca ttggataaag acttaatagt tgtgttggat ttacaccatt ttgaagaaat
     9901 tatgaaagaa ccaagagttc ataaagaaaa gtttttaaaa atatggcaac agatagctaa
     9961 taggtatcaa aaatatgata aaaaattggt atttgaatta ttaaatgaac ctaaagaaaa
    10021 tttatcttca caattactaa acgaatacat tgaagaagca ataaagataa ttagaaaaac
    10081 taaccccaaa agaactatta tagtaggacc atataatttc tatcaaattg attatttgaa
    10141 tgaattaaat attcctaaag attctaatat agtagtttct tttcactatt acgagccaaa
    10201 tgattttgca tttcaaggaa atatctatca taagggattt gagcacttaa gcaatattac
    10261 ttgggaagga acaaatgaac aaatggatta tttaaaaaaa aggtttgata ctgtggaaaa
    10321 ttgggcaaat aaaaataagg taaaaatctt cttgggtgaa tttggaataa caaaagaggc
    10381 accagaagtt tcaagaagag catggataaa agctgtaaga gaagaagctg aaaaacgaaa
    10441 cttttcatgg gcttattggg agcttgcatc tggatttggt atatacaatc aaattgaagg
    10501 gacttgggat agagatatat taagtgcatt aatagaaaag aggtgatttt atgaagaaaa
    10561 attcaaaaaa gatacttata actttaacta ttattactaa tgtcatatat atattgtgga
    10621 gaatttttta cacagtacct aaagaagaag ggaagtttgc cttaatatgt gccataatac
    10681 ttctttttgt agagataatg ggtatgatgg aaatgtttgt gcattattat ggtatgtcaa
    10741 atattgagta tcctgaaaaa ccaatcataa gtgaagaact ttatccacat gtagatgtat
    10801 ttattgcaac atataatgaa tctgttgatt tggttagaaa aactgtaaat ggatgtattc
    10861 atatgcagta tccagataag aaaaaggtac atatatacat atgtgatgat ggaaatcgtg
    10921 aagaaatgcg tattctagca gaaaaaatgg gtgtaaatta cataactaga actgaaagag
    10981 aaggagcaaa agctggaaat ttaaataatg caatgcaaca tacaacttct cctttgattg
    11041 ctacatttga tgcagatatg ataccaatgc atgacttttt aattgctaca gtaccatact
    11101 ttttaaaaaa tgagcaagct aaaaaagatg gagaaaaaga ggagtatgaa aaggttggtt
    11161 ttgtgcaaac tccacagagt ttttataatc cagatttgtt ccaatttaat ctgcactctg
    11221 aaggaaggat tccaaatgag caagattatt tttacagaga tattcaattg gctagaaata
    11281 ggacaaattc tgtaatttat ggtggtagta atacagttat ttcaagggaa gcacttgaag
    11341 aagttgatgg attttatact tattctatta cagaagattt tgcaacaggt atcttaattc
    11401 aaagtaaggg gtatagatgt tatgctattc cagaagtaca tgcatcaggt ttatctccta
    11461 cagatttaaa aagtcttatt aagcaacgtg aaagatgggc acgtggatgt atacaaactg
    11521 gtagacgtct aaatattttg tttaggagag gtttaggttt ttggcagaaa ataagttata
    11581 tttcttcaat tacttattgg tatgcaagta taaaacgctt tgtatatata atggcaccta
    11641 ttttattttc tgtgtttaat gtgattgtag taaaatgtac gttacttcaa gtattggtat
    11701 tttggttgcc aatgtatatt ttaagtagtt tatctcttaa aatattttct caaaatataa
    11761 gaaatactag atggaccaat atatatgaaa ctataatgtt tcagtcttta atgccagcag
    11821 taatacttga aacgtttgca atatctaaaa ataaattttc agtaacaaat aagagtaaat
    11881 tagaagaaaa tagaatgtat aagtttttac agggaatacc ttattttatt tacatggtct
    11941 tatctatcat aggaatttta aaaatgtttg tagcaatatt taaaatgagt tcaatgacat
    12001 attcagttgt attattttgg ttgattggaa acctttttaa ccttgttatg gctacattat
    12061 ttatatcagg gagacagcag ttaagaaagt ctgagcgtta tattgctgaa attgatttta
    12121 agttaaagca aaattcttat gttctttctt caaaaacaat tgatatatca gaaaatgggt
    12181 ttgcattttt gctagaaaat ccagaatata tttcaccaga agaagagttt gaggtagagt
    12241 ttagagaaaa atctggaaat gaaatgtata tagccaatat gaaagcaaag attgttaatg
    12301 ttgtggaagt aaattcaaag tggaaatatg cagcttatat tacacatatt gaagatagtg
    12361 aaatagataa ttggatgtgt attgttcacg atagaatacc tactttacca atgacaatat
    12421 ctaatcaact aggtttcttt gatgatttgc aaattaatgt gaaaaaacgt attgaaaaaa
    12481 ctagaacatt atcacgaaga agtccaagaa tcaatatgaa ttttcagatg gatataaaaa
    12541 atacaggtaa attaagaata gtgaatttta attatcaata tgtattatta aattttgaga
    12601 ataaaagtat ttatcctaaa gaaatagcat tggaaattaa tgaaaatatt gtgttagaat
    12661 gtgatttgtg tgagggtaaa attgatgaaa gaggtatctt atataaagta aataacattg
    12721 acagcataat gcaaaatttc tttttaagag atgaaatgat ggattggata ttacaaaata
    12781 agacaattct tgtttcaaag cctagtgaaa agaaggaaaa aagtatagat gagtttgaac
    12841 ccatggaata tatttaggag agatgaaatg aagactttaa aagtgttttt ttgttctaca
    12901 aaatatctta ttttggtgtt tatttttttt ataaatacta cttttataag ttcagcaaca
    12961 agtgataaaa ttttagattt gtcctttaaa aagatagaga ctgacttaaa ttctaagatt
    13021 acatatgaag atacaggtgt aaaaatagaa acggatagtt ctaaaagcga taaggaacgt
    13081 tatttatata tttaccaaaa tataaaagaa aattggagca tgtataataa tttttatatt
    13141 gaaattcaaa ataaaaataa aagttctcag agaataaatt taagcataca aagtaaaaat
    13201 atgcttgaat ttagattaaa agaaggtagt gaagtatttt tagaaggtaa aaatataata
    13261 tattcagata aaataaaaga aggatgcata gaagtaccag gtgaatttga aggcaaaatt
    13321 tatgtgaact ttaatagcct tataaatgag gaaagtaatg ttgtgctgga ctcaaatatg
    13381 ctttcaaaca ttgttagttg gggaattacg tttattccat ctgatgaaga acataatatt
    13441 gttattataa aaaagatatc tttattgtct gaagagaaat taagattttt aaataatatt
    13501 aaaataattg gcgatgaaga agtacaaata ccagtattgg gtcaaagtat aagtcagtat
    13561 gaagtattag gtcttaaaag tgattctaaa attaagtact ctcttatggg aaaacaagat
    13621 aatgtaagca tatctcaaaa aggtaaatta acattaagta ataaagccaa acctggacaa
    13681 attattttac aagttaatgt tgatgataag tttaaaattg gaaagaaaat aacattaact
    13741 gagtcttgga gtgttaataa aaaagataag gatggtgttc catatacatt agtatatcct
    13801 gagcaatcac caacagtaca agatatgaaa aaaataaact ttatgaataa catcataacg
    13861 tttgttcgaa ttttatttgt atctctagta ataatatgct ttggaatata tttatattgg
    13921 aaaaaatgtt caaaaactaa gtaaagagag gaggttagat tgtgttattt tcaagtttaa
    13981 tttttttatt ttatttttta cctataactt tggttttata ttatgtattt aggtttaacc
    14041 gaacgataca gaacatgata ttgcttgctg ttagtttatt tttttatgct tggggagagc
    14101 caaaatttgt tgttatcatg atagtttcca taataatgaa ctatatattt ggtttactgg
    14161 tagatagata tagggaaagt aaaataaaag taaaagtttt tttagttttg atgtgtgcat
    14221 acaatatagg tgttttattt atatttaaat atctagcttt tgcgctaaga aatattagtg
    14281 aacttattag tactgaattg actataccta gtattgtttt acccatagga atttcatttt
    14341 ttacatttca gggaatgtct tatgttatag atgtatatcg tagacatgga gaagtccaaa
    14401 agaacccttt ttatgtggga ttgtatatag catttttccc acaattaata gcaggtccta
    14461 tagttagata tgagagtgtg gcagaacaaa ttctcaatag aaaagaaact tggaataaat
    14521 tttctattgg gacttgtagg tttataacag gtctaggtaa aaaagtatta atttcaaata
    14581 acatggctat agtagcagac tatatttaca ctatgaatag tcaaggagaa atagctgcat
    14641 cacttgcatg gcttggttca atagcctata ctctgcaaat attctttgat ttttctgctt
    14701 attcagatat ggctataggc ttaggtctta tgtttggttt taaatttgaa gagaacttta
    14761 attatccata tatatcaaaa tcaattagtg agttttggag aagatggcac atttctttgg
    14821 gaatgtggtt taagagctat atatactttc cgcttggagg ttctagagta gcaaataaag
    14881 atattatgat tagaaatatg tttatagtgt ggttatttac tggaatatgg catggagcag
    14941 aatggacatt tgtaatttgg gggattttaa actttgtttt cctaattata gagcgattta
    15001 taatgtttga aaagattgaa aatcacaatt ttataaaaca tatttatgct ttattagtag
    15061 ttaattttgg gtgggtatta tttaggtcac ctaatttaaa agaagcatat aattatttta
    15121 aagctatgtt tggaggcaat ggagctatat ggagtgatta tacatatatg tttttaaaag
    15181 aatactgggt tttctttata tttgcattta tttttagtgt acctattgca aagaaaatca
    15241 ataagtttgt tgttgaacaa gctaaatata gtatgatatt taacgtattt tatccaatct
    15301 cagctatttt attgtttttt atttctgtga catatttagt aactggaagc tataatccat
    15361 ttatttattt taatttttag ttgggaggaa aagtatgaat agatttttta aagtaagatt
    15421 aataactgca attatgttct taattatttt attcgctttc tctatcttta atgtttgtta
    15481 ttctggaaaa gatacgatac aggaaataaa aaattcaata gctgaaaaaa gtagtttaaa
    15541 agatacaata tcatctattg ataatacagt gaatgacaaa gtaatataca aaaatacgtt
    15601 cattgaaact tatggctatt tacaaaaatt aatgggtaaa aatgagtttt caaaatttag
    15661 tgttgtaaaa gatacttctg gaaagatgca ttatacatat tttgctaaag ctccaaatcc
    15721 agttggtgtg atagcagata gggtaagaaa attttctgat gaaatggaaa agcaaaacac
    15781 taaattggtt tacctaatga ctccagataa gtatataaaa ggagtgacaa aatttccaaa
    15841 aggaatacct tattcttata gtaatgagac agcagataat tttttaaatc aactaaaaaa
    15901 gtataatgtg gactatattg attttagaga aaatatttta aagagtggta ttccaaaaga
    15961 tgatttattt tttaaaacag accatcactg gaaagtagaa acttcttttt gggcatttgg
    16021 agaattagtt gagcaactta ataaaaaata caatatgaat ttagatgaaa atcattatta
    16081 tagagataag gacaattata attcaattgt atatcctaag tcatttcttg gttctatggg
    16141 aagaaagact ggtatcttat atggtggtat agatgatttt acattaattt atcctaaatt
    16201 taagactaat tatacttatt atacagattc taaatctcaa aaatttgaat taaaaggaag
    16261 gtttgaagag agtttaatat tatcatatcc ttttaatgca gaccttgatt taatggatgg
    16321 tcaatcagat aagtatttta catatttatt ggggaacaga ccattagtta aaataaaaaa
    16381 tatagaaaat ccagatggat taaaagtgct gtttgttaaa gattctctaa ttgtaccaac
    16441 agcagcattt ttttcctctg tttgttcatc tatagatatg atagacccta gatattatga
    16501 tggagatata ctagaatatg caaaatccca tgactatgac tttgttttta tatctgtata
    16561 tccccaaaat cttacaaaag aattttttcc gttttgtgag taaatttcta aaaaagtttt
    16621 ctgataaaat aaatggctaa ctggtaaaaa gttagccatt tattttgagt gttattctaa
    16681 gaaatatttt ttttactaaa tacttcttta tctatttttc caagtgattt tcctacagta
    16741 attgatgata ccatagcacc attaacattt atcatagtac gtcccatatc tagtataggg
    16801 tctattgcaa gtataccacc aactaatgga aaataagaac caagaccaac tccagagata
    16861 acaactgaaa cggccattgt agcagttcct ggaagaccag ctattccaaa cgaactaatt
    16921 gttataacaa ttaacaacat aacataaaag ctcatatcca tgtttactcc agccatattg
    16981 gcaagtgtta cagccattaa agcaggatat atacctgcac aaccattcat acccatattt
    17041 gcacctaaac ttgctgtaaa gcttgataca ccctcatcaa ctccgtgatt ttgtactaga
    17101 ctttctatcg ttacaggcaa tgttcctaaa ctagaacgtg aagtaaatgc cagaagtaaa
    17161 gcttcagttg aattttttat ataagtaatg ggatttagac catttaaagt tataaaaatt
    17221 aaatgtataa taaataatat tgcaactgat atataaagag ctaatataaa ctgtaaaaca
    17281 gatactattg aactaatacc ttgtgaagtt attgtatttg caagcagagc aaccacagca
    17341 taaggcataa atttaataac agtcatagac acacttacaa ttattttgta agatgcttct
    17401 ataaaaccta taaatggttt tattgtttca aaatgttttt tacttaatct ctttattgag
    17461 gtagctataa atgtagcaaa tattattgta gctacaatat ttccatttgc catagccatt
    17521 acaggattag atggtagtaa tcctcttaaa gtatccacaa gagagtttat ttctttaagt
    17581 tcttgatttt caactaaagt aggcatatta gctcctacac ctaacttcat tatatttgca
    17641 acgataattc caacaattgc agctatagca gtagtagcaa gaagcatacc taaagattta
    17701 aatgtcagag ttccaagatt gttatctttc atatttataa ttacttttat aatggataag
    17761 aaaactaaag gtactactaa catttttaat aagtccataa atccagaacc aaataggcta
    17821 taccacttac ttacttcttg tagccaaatg acttgagttg tatcattagg aaaaccagca
    17881 aaagcttgta ttatgattcc caatatgagt cctagtactg tagcatatat tgttctcaat
    17941 gaaaatttta tttttttctt ttcaaattgt ctaacaataa agaacgtacc tagaagtaat
    18001 attatgaaaa aaattgtttt aaaatctgta atcataagaa attgtgataa aaatgaactt
    18061 tccaatattt ttctcctttt atttaaaaat aaatttttct aagtttaaat gatatcaata
    18121 tattaaaaaa atgtcaatat tatatcacaa tttacttaaa tagagtaaaa caagttgatt
    18181 ttgaaaaatg tgattaagta cagaaaaata gggtataata aatgtttaaa gaatttgaat
    18241 tgtggtaaga aaataaaaaa tagcttttat aagatttgat ttcaaattaa aatgtgttaa
    18301 ataaataact aaaaattatt ataaaaaatg ttttcataga atatatatca aatgaaaagc
    18361 ttaaaataag tgaagaaatt aactagacat caaaggagag ataaaatgag agtaataata
    18421 attggtggtg ttgcagctgg tatgagtgct gcagcaaaat taaaaagaat aaaacctgag
    18481 tatgaagttg ttgtttatga aaaaactgaa atagtatctt ttggtgcttg tggattacca
    18541 tactttgttg gtggattttt taatgatgct gatgaattat tagcaagaac accagagaaa
    18601 cttagagaag ctggaattga tttaaatata tttagagaag tcgttgaggt tgatagtgaa
    18661 tctaagaaaa taaaagtaaa aaacataaaa acagatgaaa tatatgaaga ttactatgat
    18721 aaattgatga tagcaactgg agcaagaagt attatgcctc ctataaaaaa cataaaatta
    18781 aagaatgttt ctacattaaa aagtttatat gatggagaat atttaaaaaa acttttaagt
    18841 aatgaagata ataaaagagt gacaataata ggagctggtt ttatagggtt agaagcagtt
    18901 gaggcatgta agaaattagg taaagatgta gatgtaattc agttagaaga tagaatattg
    18961 ccacaagtat ttgataagga aatgacggat gtattagaag aagaaataaa tagacataat
    19021 gttaatttgc acttagatga aatggttgta gaactttgtg gagaagataa agtagaaaaa
    19081 gttataacaa acaaagggga aatagataca gatgtagtaa taattgctac tggcgtgaga
    19141 cctaataccg agtttttgtc aaatactaat ataaaaatgc taaaaaatgg agctatagta
    19201 gttgatgagt atggaagaac ttctgtagaa gatatatact ctgctggaga ttgtgcaact
    19261 ataaagaata tagtaagtaa tgaaaatgta tatgttcctt tagctactgg agctaataaa
    19321 ttgggtagga tagtaggaga aaacctagca ggaagagaaa tatcttatca aggttcacta
    19381 tcttcaagct gtataaaaat tatggatatg gaggcagcat caactggaat tacagaaagg
    19441 caagctaaag atttaggcat aaatgtcaaa tctaaattta tttctgatta taatcaaact
    19501 cattattatc caggaagaaa taaaatatat gtaaagctta tttatgatgc agatacaaaa
    19561 gttattttag gcggacaagt ggctgggttt aaggatgctg tccaaagagc aaatgtattg
    19621 gcagctgcta tctttggaaa aatgaccaca aatcaacttg gaatgttaga tttatgttat
    19681 gctccaccat ttgcaagaac atgggatgtt ttaaatgtag ctggaaatgt
//
LOCUS       DAGSHT010000054        19524 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGSHT010000054 DAGSHT010000000
VERSION     DAGSHT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.055"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            338..859
                     /locus_tag="MJB72_001726"
     CDS             338..859
                     /locus_tag="MJB72_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8262840.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            893..1669
                     /locus_tag="MJB72_001727"
     CDS             893..1669
                     /locus_tag="MJB72_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8262841.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEEACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            2011..2964
                     /locus_tag="MJB72_001728"
     CDS             2011..2964
                     /locus_tag="MJB72_001728"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBY8262842.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2982..4001
                     /locus_tag="MJB72_001729"
     CDS             2982..4001
                     /locus_tag="MJB72_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBY8262843.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            4032..4283
                     /locus_tag="MJB72_001730"
     CDS             4032..4283
                     /locus_tag="MJB72_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262844.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4565..6370
                     /gene="lepA"
                     /locus_tag="MJB72_001731"
     CDS             4565..6370
                     /gene="lepA"
                     /locus_tag="MJB72_001731"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBY8262845.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6519..7856
                     /locus_tag="MJB72_001732"
     CDS             6519..7856
                     /locus_tag="MJB72_001732"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBY8262846.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8089..9402
                     /locus_tag="MJB72_001733"
     CDS             8089..9402
                     /locus_tag="MJB72_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY8262847.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMTNVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9574..10749
                     /locus_tag="MJB72_001734"
     CDS             9574..10749
                     /locus_tag="MJB72_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBY8262848.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10852..11871
                     /gene="hrcA"
                     /locus_tag="MJB72_001735"
     CDS             10852..11871
                     /gene="hrcA"
                     /locus_tag="MJB72_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBY8262849.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11901..12521
                     /gene="grpE"
                     /locus_tag="MJB72_001736"
     CDS             11901..12521
                     /gene="grpE"
                     /locus_tag="MJB72_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBY8262850.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12613..14460
                     /gene="dnaK"
                     /locus_tag="MJB72_001737"
     CDS             12613..14460
                     /gene="dnaK"
                     /locus_tag="MJB72_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBY8262851.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14671..15825
                     /gene="dnaJ"
                     /locus_tag="MJB72_001738"
     CDS             14671..15825
                     /gene="dnaJ"
                     /locus_tag="MJB72_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBY8262852.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16791..17726
                     /locus_tag="MJB72_001739"
     CDS             16791..17726
                     /locus_tag="MJB72_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY8262853.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17762..19135
                     /locus_tag="MJB72_001740"
     CDS             17762..19135
                     /locus_tag="MJB72_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8262854.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19323..>19524)
                     /locus_tag="MJB72_001741"
     CDS             complement(19323..>19524)
                     /locus_tag="MJB72_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8262855.1"
                     /translation="LLYTGIKFINNDLNISKTSTSMFLHRNTLVYRLEKINEILGFDL
                     KNFENAMIFYLSVKSYFLYKKI"
BASE COUNT         7748 a         2147 c         3539 g         6090 t
ORIGIN      
        1 ccaataatta taagataata caagaaatta ctcatttttg taaatttaat gaaaaattat
       61 aaaaatataa atttatatgc ttaattaaga taatttttcc aataatcaag ataatttttc
      121 caataatcaa gataattttt ttcattagta tgtatgatag atgcatcaaa caatataact
      181 aatatgttag aaggttatat gtaattaatg tcagtgttta tatagaatag catttgtagc
      241 atttattata gttgtaatta ctatttatat catagacttg aatagtctaa aaaaatagac
      301 tataatattt ttatagacta aagaaagggc tgataagatg gacatagcaa aactaatttt
      361 aaatcctgca aggcttcgta ttttacaata tattcgactt tatggcagtg ttcgtacatc
      421 tgatattgta aagtatttaa atgatattcc tcgtgcaaca gtttatcacc atgtaaaaat
      481 tttagaagag aacaatatga ttgaagtaat taaagaaaat cgtgtgcgtg gtactattga
      541 aaaggtttat gcactaaagg aatatacaac atctatggaa ggagagacct tcgttgcact
      601 ttctacagca ttttatgtag gtctgatgca ggaaatgaat gaatacttta gcagaaaaga
      661 gcaagaccat aaaaaggata atgtattttt ctcttcagca ttactatata tgagtgataa
      721 tgaatatgaa aatcttctta aaagtatagc tgatttattg aaaccatata tagaacaaaa
      781 acctaaatca aatctaaaac ttagaaaact ttcaattata tcttctccac ctgtagctga
      841 taatgataaa acaaaataac atgcaaccca tttttataaa aggaggttat atatgaaatt
      901 tcatgagttt ggaaataaaa acagaccaca tattatgttg attcatgggg gagggaatgc
      961 atggtggaac tatttgcgac aagcagaagt tttgtctaac aaatatcatg tgatacttcc
     1021 tacattggat ggtcatggag aagaatatga aacaacatac atttctacag aggatacagc
     1081 agataagttg atatcatata tagatgaaaa ttgcagtgga catcttttcg ctttgtgtgg
     1141 ggtatcactt ggtggtcaaa ttgtcatgga acttttatct cgaaaaccta atattactaa
     1201 aaaagcaatt attgatggta gtatttgcta cccaagacct cttatggcaa ggttttgtat
     1261 tgctggtatt cgattttttg gaggactttt gtttagtgag gaggcttgtc gctttcaaat
     1321 tgctatgatg ccaaaattgt tacctgaaaa tatgcaatat ccagatgaaa taaaagcgta
     1381 ttatatgaca gatatgccac atgtaagaaa agaaacattc tataatatgt atcgtactta
     1441 catgatgaac tatacactta aagagaatgt taaaaaaacc acagcacaag taatgtactg
     1501 gtatggagaa aaagaaatga agtgtgtcaa aaaatctgca aaactgtttc agtcttatgt
     1561 gccctcttgt gagatttatg aagctaagag ctataatcat gggtatttat cactttatct
     1621 tccaaatgaa tggttggaaa ttgcagaacc atttttccaa aaggaataaa ggtgttaggt
     1681 gattattgta taattattgc tgttttttta tatagtaaat aaattaggta taaatataaa
     1741 aaattatttt agttaatata ttagtgaaag cttaatagga aaattataaa tagattaaag
     1801 gtagcaccta tataaatagt tgctaccttt ttctgtttgt aaataaatga tagataagaa
     1861 ggtttactga caaatcactg actaaaccca attttcttac attgaaagcc ttaattttac
     1921 ttgttttaaa taaatttaca atttcttgtt ataaaatact aaattttgtt aacaattata
     1981 attagcattt aatttatact ggaggtttat atgattagtg taagaacaga tttagcttta
     2041 gaagctagtg aaatgtgtga aaagtctcaa gaaggaagta gtattcctgg tgtaaaaata
     2101 gaaactaaag aattagaaaa ttgcattgta actaaagttg aagtaataga caggcaaggc
     2161 gcagagataa tgaataaaga tataggaaag tatataactc tggaaagtaa tcttatgaag
     2221 tttgatgatg atgagtcaag agaagaaatg ataagttatt taaaagagga gttagtagat
     2281 atatttggcc aagacaaaaa taaaaaaacc ctggtaattg gtcttggaaa tagaaatatt
     2341 acttctgatg cgttaggacc aaagagtgta tcaaaaacac ttgtaactag acatcttttt
     2401 aaaaattata ataaagacta tgatgatgat ttcactgagg tatcagcttt aagcccagga
     2461 gttatgggtg ttactggaat agaaactagt gaaatagtaa aatcattggt tgagaaggtt
     2521 aagccagata gagtagtagc aatagatgca ttggcttcaa gaaaaatgga aagagtaaac
     2581 tctactatac agatatcaac agcaggaata tcacctggtg gaggtgttgg aaatactaga
     2641 aaatctctaa ctaaagaaac tttaggcgtg gatgttattg caataggcgt tccaacggtg
     2701 gttgatgcag caactcttac aatagatgtc ttagatatgg caatagacaa tttaatagct
     2761 caatcagaag aaacagaaag tttttatgaa atgttgaaaa aacttaaaga agaagaaaaa
     2821 tatcatctaa taaaagattc gttagaccca tatgacaaga acttaatagt aacaccaaag
     2881 gatatagatg atacaatcga aaatctatct ataataataa gtgaaggctt aaataggtct
     2941 ttacatccag gtagattggt ataaaataag gaggagcaat tatgtttaaa aaatgcatta
     3001 aagtagtaac cttgactttt atattagtat gcattttacc aggaaaatca ttagctttga
     3061 atcaagatga ttttttaaag tttttagtaa attcatctta tccagaagct aaagtcgaag
     3121 gaaatgatac agaaaataaa aagaataata aaaataaaga aacaagtaag gaaaataagg
     3181 aagaaagtaa agaaggaaac actaaatcta aagatgcaag taaagtcgat aataaaaaag
     3241 agagtgaaaa agaatatata aaactttatg ttggtaaaga aaatgttcca gatattgagt
     3301 caaaaaattc agacacaact gaaactaaca caacatctag ttcagattat aaggatgatt
     3361 taagagtaac caaagaaaat ccaagaattt tgatatatca tactcatgga tgtgagactt
     3421 attcaaattc acctgatgga aattatcatt caagagataa gaaaaattca gtaatggaag
     3481 taggaagtgc attaactagt gctttagata gcaaaggatg gggtgtagtt catactacta
     3541 aataccatga ctatccatct tataataact cttatgcaag tagcttaaaa acaatacaaa
     3601 gtatattacc taaatataac tcagtggata tagcgataga cttacataga gatgctaggg
     3661 atttaactaa tccagccact aaggaaaaag accatttaaa atatacaact atgataaatg
     3721 gagagagagt atcaaagttc ttctttgttg taggaggtaa aaatacaaat agaaaacaac
     3781 ttagagcttt agcagaagat ataactgctt ttgctgagaa aaagtatcct ggtctagtat
     3841 ctccaatagt agaaaaagac tatgctagat tcaatcagtt tgcagttaag aatcatatgt
     3901 tagtagaaat agggaataac gcaacaagtg tagaagaatc taaggctact acaaagtatt
     3961 tagccgagat tttggatgaa tattttaaac aaaaaaatta gagctttaag ttataattag
     4021 gagcatgcta aatgagccga ttagaaaaaa atatagaaag taaaagtaaa ctaagaaaat
     4081 acaagtatat tatgagaatc ttatttttga ttactatgat tataggtaca tcagtttgtg
     4141 tatttatagt ggacagtaat gcaaaaagta tgctaggaat gaaacaatat gattttccaa
     4201 gtattaaatt tgatataaca aaatttgatt ttgacatacc aaattttgat atacatgaaa
     4261 tcataaaaaa atttaataag tagtaataag tgaataaggt ttattgatta aaataagcct
     4321 tattttagtt atatattttt aatgtataaa ataaaaattc attaactgta ttttatagga
     4381 tttactttaa gtttacgata ttatataata tttagtgtta gacttgataa tccacatata
     4441 atgtggataa ttttgtttag tgccaattag tttatattca aaaattttaa atttggtata
     4501 taaagagttt ttagataatg taaaataata actataagaa taatcaagag gagggaattt
     4561 taaggtggac aataaacaaa gtagaactag aaattttagt ataattgcac atatagacca
     4621 tggaaagtct accttagctg atagattaat acaacaaact gggcttgtta gtgaaagaga
     4681 catgaaaagt caattacttg acaatatgga ccttgaaaga gaaagaggaa tcactataaa
     4741 gcttcaaaac ataagattaa tgtataaagc gaaggatgga aatgaatatt atttaaatct
     4801 tatagataca cctggacatg tagactttaa ctatgaggta tcaagaagtt tagctgcatg
     4861 tgaaggagca ctattagtag ttgatgcagc tcaaggtgtt gaggcacaaa ctttagccaa
     4921 tgtttatctt gctatagacc aagatttaga aatattacca ataataaata aaatagattt
     4981 accaagtgca agaccagagg aagtaaaaaa tgaaatagaa gatttaatag ggcttgattc
     5041 aagtgaagct cctcttattt cagctaaaac tggtttaaac atagaagatg ttttagaaga
     5101 tattgtaaaa aatgttcctc cacctaaagg agataatgaa gctccattaa aggcattaat
     5161 attcgattct tactatgatg cttacaaagg tgttgtagct tatgttagag tatttgaagg
     5221 aactgttaaa aagggtatga caataaaaat gatgaatacc aataaaaagt ttgaggtaac
     5281 tgaagttggt gtcatggctc caggtcaaac tgaacttagt gaattatcag caggtgatgt
     5341 tggttatatt gctgctagta taaaagatat aagaagttgt cgtgttggtg atacaataac
     5401 agattcaaat aatcctacag aagaaccttt accaggatac aaaaaagcta ctccaatggt
     5461 atattgtggt atatatccag gagaaggaga aaagtatgag aatgttagag atgctcttga
     5521 aaagcttcaa gtaaatgatg ctgcacttga atatgaagct gagacttcag cagcactagg
     5581 atttggtttt agatgtggtt tcttaggtct tttacatatg gagataatgc aagaaagact
     5641 tgagagagaa tttaaccttg atataattac tacagctcca tctgttatat atagagttac
     5701 aaaaatggat ggagaggttg taatgataca aaatcctgca aacttaccag aaccatctga
     5761 gataaagatg atagaagagc ctatagtaaa aggagatata atagttccta aagactatgt
     5821 gggtgttgtt atggagcttt gccaggaaag acgtggaaat atgttaaata tggaatatat
     5881 agatgagaga agagtaatgc ttcactatga ccttcctctc aatgaagttg tttacgattt
     5941 ctttgatgct ttaaaatcaa gaactagagg ttatggttct cttgattatg aggttaaagg
     6001 ttatgtagca tctacacttg taaaacttga tatattaatc aataaagagc aagtggatgc
     6061 acttagtttt attgtccatg agacacgtgc tttccctaga ggaaaggcaa tgtgtgagaa
     6121 attaaaagga gaaattccaa gacatcagtt tgctatacca atacaagctg ctgttggtaa
     6181 taaggttatt gcaagagaaa caataagcgc tctaagaaaa gatgttcttg ctaaatgtta
     6241 tggtggagat atatctcgta agaagaaact tcttgaaaaa caaaaagaag gtaagaagcg
     6301 tatgagacaa attggttctg ttgaagttcc tcaaaaagca tttatgtctg tgcttaaatt
     6361 agatgagtaa atccttataa atttagataa agatatttaa aagctagagt gattttagaa
     6421 ttatttctaa attagtctag cttttttatg gtatatttta agtaaactca tagagaatat
     6481 aagtaagaat attaaagaag aataaaaagg tgatatttat gattaatata gaaattccta
     6541 aaaaagtaga ctatataatt aaagaattag aaaagaatgg ttatgaagct tatatagttg
     6601 gaggatgcgt tagagattgt ttattagaaa gagttccaaa cgattgggat ataacaacta
     6661 gtgctagacc agaagtggta gtagaattat ttgaaaaaac aattccaact ggtatacaac
     6721 atggtactgt aacagttatg attgaacatg aaccatttga agtgacgact tatagaattg
     6781 atggaaatta tagtgatggt agacatccag attcaataga atttacaaat aacatagtta
     6841 aggacttgtc aagaagagat tttactatca attcaattgc atacaattca aagacaggat
     6901 tagtcgaccc atttaatggc tatgaagata tacaaaataa gtatatacgt tgtgttggaa
     6961 atccagtaga tagatttgaa gaagatgcac ttagaatgct tagagcagtt agattttcag
     7021 cacaactcaa ttttaaaata gctgaaggta ctaagcaaag tattcataaa aaagcagatt
     7081 tgataaaaaa tgtttctatt gaaagaatcc agacagagtt taataaaata ttagtatctg
     7141 attcatctaa attaaactta ttaaaatcaa caggtttgtt gaaatttata attcctgaaa
     7201 tatgtgaatt agaagatgtt actcaacaca atccatatca catctatgat gtacaaaaac
     7261 atactttaat agctacagaa gtaattgaag atgaactgta tttaaaactt acaatgttgt
     7321 ttcatgattt aggtaaaaaa gtaactaaaa caactgataa aaatggtgtt gaccattttt
     7381 atactcatag tagagaatct gtaaagattg caaagaagat tttgaaaaga cttaagtatg
     7441 ataattatac tattaataaa gttttaatat taatacaata tcatgattat agaatagaac
     7501 ctaaaaggaa gataattaaa aagttattaa ataaacttga agatgttgga ttatttgaag
     7561 atttaataaa agttaattgg gctgatacac ttgccaaaaa tccaaagtat gcaaaacaaa
     7621 agatattaaa tcttattgag tgtgaaaaag aatttaaaca tataataaat caaaaggagt
     7681 gctttaatct taaagattta gctattaatg gaaaagattt gatatctata ggtataaaac
     7741 caggtaaaga tataggatat attttaaata agatgcttga aatagtcata aataatccag
     7801 agttaaatga aaaagaaata ctaaaagaaa aagcattaaa tatatataca ttttagattc
     7861 aaatataaaa aatttaatta ctttaaaaaa catagaataa aatacttatt tagataaaat
     7921 aaataatttt tagagaatat tttagaagtt acgattgtat gatttttaaa tgcataggca
     7981 tttactctag gactatagac taatataaaa aaaaactttg ggaatactaa aaccataaat
     8041 ttataaaatt aatattttat atactgtaaa acagtgagga gggacagtat gcatactcaa
     8101 ctacaaaaga ctattggtct ttcagcagct ctttctactg ttgtaggtat ggttataggt
     8161 tcgggagttt tttttaagcc acaagcaata tacactacaa ctaatggagc tccaggtcta
     8221 ggtattatag cgtggctttt aggtggtttt ataacaataa ctgctgggct tacagctaca
     8281 gaaatttcag ctgctatacc aaaaactggt ggtatgatga tttatatcga agaaatatat
     8341 ggagaaaagt taggtttttt aactggatgg atgcaaactg tattattttt cccaggtaca
     8401 tcagcggcat taggagtaat atttgctcaa caagcatctg aacttttagg aatgagccca
     8461 aataatatga caaatgtact tcctatagca attggagtta tactattttt agcattatta
     8521 aatataatag gttcatcctt aggaggaaaa gttcagacag tagccactat tggaaagatg
     8581 atacctttaa ttttaataat agtctttgga tttattaaag gtcaaagtag tgaagtatta
     8641 aatccatttg taggagatgg tgtaaatgca tcaaatgcac ttggacaagt attaatagct
     8701 actttgtttg catatgatgg ttggataaat gttggagcca tatcaggaga aatgaagtct
     8761 ccagagaaag atttaccaag agcaatagtt ggtggtttat cattagttat ggcagtttat
     8821 attataataa atatagctta tctttgggta gtaccagcaa gtgaattagc aacagttact
     8881 tctccagcta cattagtagc aacaagattg tttggaaata taggtggaaa agtaataact
     8941 gtgggtatat taatatcagt atttgggaca ttgaatggat atttattaac aggttcaagg
     9001 ataccttata cattagctga aatgggaact ttaccagcat ctaaaactct tttaaaagta
     9061 aattctggag gttctcctgt aaattcaata ctacttataa ctgtgttagc ctgtgtatat
     9121 gcattatctg gtcaatttaa tcttttgaca gacttaacaa tattttcaat atgggtattt
     9181 tatgtattga cttttatagg tgttatgagg cttagaagac aaaagccaga tttacacaga
     9241 ccatataaag tccctttata tccaataata ccaattatag ctatattagg tggactgttt
     9301 gtaataataa atcaaatatt gacttcaaca gtcatatctt taggcggaat atttataaca
     9361 ctattaggtt taccagttta ttattatatg aaaaaaagat aactacttaa tttaagttag
     9421 ataaatttgg gtaaaggtga aaattaactt ttatccaaat tttttatact gttatacaat
     9481 ataagtatta tatattgctt gatgtgctat tttgtttgat ttataattta gtaattgatg
     9541 ttttaagata atttaagaaa tgaggagtga aagatgctag gactttatgt acatattcca
     9601 ttttgtgtga aaaaatgtaa gtattgtgat tttaattctt ataaaatgga catagattca
     9661 aaaaaaagat atatagaaga cttaaaaata gaaatggaat tatatagtaa taaattatat
     9721 aaagataata aatataaaaa taaagaatgc tgtagtctaa ataaaaatga caaaataacg
     9781 agtatatttg tagggggagg tacacctagt atactaacct ctgatgaaat aagagaagta
     9841 tttataagta taaaagaaat gtttgatata gatgagaatg cagaaataac tatagagtgt
     9901 aatcctggga cattaacatt agaaaaatta aaaactatga aagagattgg cataaataga
     9961 cttagtatag ggctacaagc tattcaagaa aagcatttga actttatagg aagaatacat
    10021 acttatgaag aatttgagaa aaactataaa gatgcattaa gtgtaggttt taaaaatatt
    10081 aatatagatt taatgtacag tcttccaaat caaactctat gtgattggaa ggaaactctt
    10141 gaaaaagttg tacacttaaa tccaacacat atatcagctt attccctgat actagaagaa
    10201 ggaactgaac tatacaatat gtatgaaagt aataaatttg aacttataga tgaaaatgta
    10261 gatatagaaa tgtatgagta tacaataaac tacttaaagt caaaaggata taatcaatat
    10321 gaaatatcaa actactcaaa agaaggctat aattgtgagc ataatatttt atattgggaa
    10381 tgtgaacatt atatagggat tggagcagga gcttctggct atattaatga gaacagatac
    10441 aataatgtag aatctttaga agattatcat ttaagtttgg taaaaagaga aaaaccaata
    10501 caagaaaatg aaattctttc tgagaaagat atgatagaag aaaagatatt tatggggctt
    10561 agaatgaata aaggcataaa atttgaagat tttaaaaaaa aatttggaat agattttaga
    10621 gagaagtata ataagcaaat agaaatgcta ttagctagaa agcttataaa tcagagcttt
    10681 gaaggtatac aattaactca aaaaggaaga gaaatatcta atagtgtatt tatagaattt
    10741 atggaataat ttttaaggtg ttgacaaaat aaatttataa tggtatataa tatatataat
    10801 ctttttagca ctcgaactta gtgagtgcta acagctagga ggtataataa tatggaatta
    10861 aatgaaagaa aattaaatat cctcaaagct atagttaaag attatataga aacagctgaa
    10921 gctataggtt ctagaacaat atctaaaaga catgatttag gtgttagtgc tgctaccata
    10981 agaaatgaaa tggctgacct tgaagaatta gggtatttaa ttcagcctca tacttctgcg
    11041 ggtagagttc catctgaaaa aggctataag ttatatgtaa actctcttat gagtaaaagc
    11101 gaattggatg ataatgataa gattcttatt gaacaatgta tgaatcataa tataaatcat
    11161 ataaaagaat tgattcatga aacttctaag ctattatcgc aactaacaaa ctatacaact
    11221 gtagcagtta ctaagagttt aataaatcaa agtgttataa agcacataca gttggttgct
    11281 atgaatgaca ataatatttt acttatagtt gtaacagata aaggggactt aaaaaaagct
    11341 aaccttacaa ctaatgtata tttagatcaa tctaagttaa atttgatttc tgataatctt
    11401 acaaggaagc tcttaggtaa aagtataaca gatttggatg ataatctaat tgcttttatt
    11461 aaatacgaaa taagtgagta ttctgggctt atagatgagc ttttaaatgc tttgaactca
    11521 aatatgaaag aagaagattt ttctctatca ttaaatggag ctactaacat atttagttat
    11581 ccagaattta atgatgtatt aaaagcaaag tcatttttaa atatgttgga aaaaaaggaa
    11641 acaatagctg acataattaa atcaaaggga atacaaaaag acaatctaaa tataataatt
    11701 ggtagtgata atgattgtga actggcacag gattgtagta tagttacagc aacttataat
    11761 gtagatagag acttggttgg gcgaataagc tttataggcc ctaccagaat ggattatgct
    11821 agaatttact ctataataaa ttatatgagt ttattaatta atagaaagta aagaaatttt
    11881 aaattgcgag gtgtaaagtc ttggataaaa aaaatgagca acaggaagta agagaagaaa
    11941 atgatacttc tattaaccaa gaatcggaaa ctcaagtaga gttagaagag gaagttgtta
    12001 atgaagaatg tgaaacatca agtgaaaaaa ctgatgaaaa agaagttgat gatgaaaatg
    12061 taacagatat aaattctaag ttagcagaaa aaaaattgca ggatgagttg gatgaattaa
    12121 atgataaata tcaaagactt caagccgaat atgcaaacta cagaagaaga actcaacaag
    12181 agaaagagac aataggcgta tttgcaaatg aaaaaataat aactgaattg ataccagtta
    12241 tagatagtat ggaaagagcc ttagatgcat gtgaagataa ggaagatact atgtataagg
    12301 gtataagcct tgtacataaa caattaatag acactttagt aaaatttgga gtagaagaaa
    12361 tagaagcaga atctaaggaa tttgacccta atctacatct agcagttatg caagaaagtg
    12421 tagatggagt tgaagcaaat caaatagtaa tggtacttca aaaaggatat aagctaggaa
    12481 ctaaagttgt aagaccttca atggttaaag tttcttgcta agtataaatt aaaggttaaa
    12541 ttatttaacc taatataaaa tattaaaata agtaaaactt aattatataa ttactcgagg
    12601 aggattttag tcatgggaaa aataatagga atagatttag gaacaacaaa ttcatgtgtt
    12661 gcagtgttag aaggtggaga agcacaaata atagcaaata gtgaaggtat gagaactact
    12721 ccatcagtag tagcatttac aaaagatggt gaaagaatag ttggagaacc tgcaaaaaga
    12781 caagcagtta caaatgcaga taaaacaata acttctataa aaactcatat gggaactgat
    12841 tataaagtaa atatagatgg aaaatcatat actccacaag aaatatcagc aataatatta
    12901 caaaaattaa aatcagatgc agaaagttac ttaggacaaa cagtaacaga agcagttata
    12961 acagtaccag cttactttac agatgctcaa agacaagcaa ctaaagatgc tggtagaata
    13021 gcagggcttg atgttaagag aataataaat gagccaacag cagcagctct tgcttatggt
    13081 atggataaat tagaccaaga aaagaaaata ttagtatttg acttaggtgg aggaactttt
    13141 gacgtatcta tacttgaaat aggagatgga acttttgaag ttttagctac agctggtaac
    13201 aatagattag gtggagatga ctttgaccaa atagtaatag attatttagc agaggaattt
    13261 aaaaaagctg aaggtgtaga tttaagaaat gataaaatgg ctcttcaaag attaaaagaa
    13321 gcagcagaaa aggctaagaa agaattatca tcaacaatga gttcaaacat aaacttacct
    13381 ttcataactg ctacagcaga aggaccaaaa cacttaaata tagatttatc aagagctaaa
    13441 tttgaagaat taactagagg tttagttgaa aaaactatgg aaccaactaa gacagcttta
    13501 caagatgcag gactttctac aggtgatata gatgatgtat tattagtagg tggttctaca
    13561 agaataccag cagtacaaga agctgttaag aagtttatag gaaaagaacc tcataaaggt
    13621 ataaacccag atgagtgtgt tgcagcaggt gcttctatac aagctggagt tttagcaggg
    13681 gatgttaaag acttattatt actagatgtt actccattat ctcttggtat agaaactatg
    13741 ggtaatgtaa tgactaagat aatagaaaga aatactacaa taccaactaa gaagtctcaa
    13801 atattctcaa ctgctgctga taaccaaaca gctgttgata tacatgttct tcaaggtgaa
    13861 agaagtatgg cttatgacaa tacaacttta ggaagattcc aattaacaga tataccacca
    13921 gcacaaagag gaatacctca aatagaagtt acttttgata tagatgcaaa tggtatagtt
    13981 aatgtttcag ctaaagattt aggtacagga aaagagcaaa aaataactat aacatcaaac
    14041 actaatttat cagaagcaga aatagagcaa aagataaaag aagcagaaat gaatgcagaa
    14101 gctgacaaac aaaagaaaga aaaaatagaa gcattcaatc aagctgaatc tactatatat
    14161 caaactgaaa aaactttaaa tgaacttgga gataaaataa gttcaggtga aaaagaggat
    14221 atagaaaaag ctatagcaga cttaaaagct gtaaaagata atcaagatgc tacagcagaa
    14281 gaacttaaga aagctacaga tgaagtaatg actaagttcc aaaaagtatc tcaagaaatg
    14341 taccaaaaag cagctcaaga acaacaagcg gcacaaggtg cagaacaagc acaagacaat
    14401 ggacctaaag atgacaatgt agtagatgct gactttaaag aagtagatga agataaataa
    14461 gctatataat atgtaacact aaatgataat tggaacatat atgatttgtg aaaatcatat
    14521 agatgttaaa gatatatgga caatatacat cataaagaat ataatatatc atagattata
    14581 taacttaaag tagaatataa gaattaatga tagcttgtag tttattattc tacaagctat
    14641 cgttttgtaa aatgaaggtg gtgtcaattt ttgagtacaa aaagggatta ttatgaagtc
    14701 ttaggtatca gtaaaggtgc agaagctcaa gagataaaaa aagcttatag aaaattagca
    14761 atgaagtacc atccagatag aaatcctgga gataaagagg cggaagaaaa atttaaagag
    14821 ataaatgaag cttatgaagt tctatctgat gatacaaaga gaaaaactta tgaccagttt
    14881 ggacatgatg ggctaaatgg tcaaggcggt tttggtggtc aaggaggatt tggcggtcaa
    14941 ggctttggtg gttttgaaga tatgttcgga gatatatttg gtgacatgtt tggaggaagc
    15001 ttcggaggtg gaagagcaag aagaagaggg cctcaacgtg gtgcagatat aagacagagt
    15061 gttactatat cttttgaaga agctgcattt ggtaaaaaga tgtctataaa agtaaataga
    15121 agtgaagaat gtgaagaatg taatggaact ggagcaaaac caggaacatc aaagaaaact
    15181 tgttcaactt gtaatggaac tggtcaagtt agaactgttc aaagaactcc atttggtaat
    15241 atagcaagtt caagaccatg tagtgcttgt aatggaactg gagaagtgat tgaatctcca
    15301 tgtagtaaat gtcatggaac tggaaataca agaaaagtta agactataga agtagatata
    15361 cctgctggta tagatgatgg tcaaatgata aaactttctg gtcaaggtga agtaggagaa
    15421 aaaggtgctc caagaggaga cttatatatt gtagttaatg ttaagtcaca tccactattt
    15481 actagagatg gaaatgatat ttattttgaa atgccaatta catttgttca agcaacttta
    15541 ggtgatgaga tagaagtacc tactttggat ggaaaagtta aatatagtgt gccagagggg
    15601 acacaaactg gaactgtatt tagacttaaa gagaaaggaa tccctagaat tagaggtaat
    15661 tcaagaggag accaatatgt taaggttgta gttgaaattc ctaaaaaatt aaacgataag
    15721 caaaaagaac tattgagaga atttgcgaag gagtgtgggt cgaacgtcca cgaaaagaag
    15781 aaaaccttcg gacaaaagat agaagatatg tttaagaaaa agtagttaaa gtagccaata
    15841 tataatcata tttttaaaat cttagttatt tataagattg tcaaaggaga tattatgatt
    15901 tttatattgg ctatttttgt ataaaaaata aatgtgcata aaataaaact gaatataaat
    15961 aattttctga aaaatacttg tcctaaaata tataatatgc tataatataa tcaaataaaa
    16021 gaaaggggtg attccagtgg gaacggatgt ggcactgcca aatgatgaag aaaactttat
    16081 tgtaatatac aaaataaaat tagatacttt tagtgctaga ttaagtactg gaaaaactca
    16141 tgagctggga taattattgt aattacctag actcaaactt ttagtagtaa aatagtaata
    16201 ttatatagac aaggtatcta atttttaaat cttttgtatg aattaaaatt tttataaagg
    16261 gtatatctga ttagaatgta attagggaaa cctagttgtg aattactttt agtatataca
    16321 tgtgtaaaga aatatgtata tggtattggt tattgtgcta gatgtgtatg taaaggaata
    16381 tgtgtctagt tgggtatatg taaagaaata tacttataat ctatgaaaaa tttttagtat
    16441 aaattgctaa aacattgaga ttgtttttta tagaaagtta gtttatacca actaaaaatg
    16501 tgcttaagat ttgtttgatg tttaattata aataaggttg tagataaaaa aataagtaaa
    16561 gacaaaggtt taaaatactt gctttaaata tagtttgttt tgaacacttg ccaaattaaa
    16621 ataattaata aaatagacat cttctcatag taaaatatac tgtgagagga tgtttttgct
    16681 ttgttcataa acttttatga aaaatttgca tagtgtcaca ttttaatata ctatatatat
    16741 aataaaatat agataaaatt aaatgattca aaagagaaag gtgaaaagag atgtattata
    16801 taggtgtaga cataggagga acaggtatac aagcaggagt agttgataac tatgggaaaa
    16861 taatttttag gtcagagtgt aaaacagtaa ttgaaaaagg ctttgaagga atacttaatg
    16921 acataaaaat aatgatttat aagttgttgg aagataataa acttactatg agtgacataa
    16981 aatctattgg atttggtgtt ccaggtttta taaataaaga aggattagta acttgtgtaa
    17041 atttaaaatg gaataaaaaa gcttttaata aagagttaaa aagaagattt ccagatgttg
    17101 aaattcatgg agaaaatgat gcaacagtag cagcacttgg agaagcaaag tttggaagta
    17161 tgaaaggtgc taatgttgga gtattatata ctttaggaac tggagtaggt ggaggaatag
    17221 taattaatca aaaagtcttt tctggagcac atggattggg ttcagaaata ggtcatcaaa
    17281 ttataggaga aaactatttt aattgtaatt gtggaaataa tggttgtgta gaaacttttt
    17341 gttcagcaac tgcaattata aagtattctc aaaaactgat agaagaagga gaaaagagta
    17401 ggattttaga tttggcagag ggaaatttag aaaatgtaaa tgcaaagatg gtgtttgatg
    17461 catatagaga aaatgattta gtggcaatta aagtaataaa tagatttaaa gagtatttag
    17521 caaaaacttt tgcaaacaca ataaattctt tagacccaga aattatatct ataggaggag
    17581 gcatttctaa atctagcgat ataatacttg atggaataga agatttggta agaaaatttg
    17641 tattatacaa aacagaagat atagctacaa ttacatgtgc aactttaggt tcagacgcag
    17701 gaattatagg agcagctttt ttataatgta aatgaactgc ttattggagg cgtgatgata
    17761 tatgggtaaa acaaatgcta gaagaacgct ttttttgata gcaataggtt caggaactat
    17821 gctgaatcct cttaattctt ctatgatttc acttgcttta catagtatac aaaatgattt
    17881 tcatattagt tttacaactg tatcttggat tgtttcagca ttttacctag ctagtgctat
    17941 tgcacaacca gtttcaggta agataggaga tttaataggt agaaaggtct tatttttgag
    18001 tggtttgatg cttgtaattt tatcagcatc catagtacca cttgttcaat cattctttat
    18061 attgattttt ataagagtaa ttcaagcaat tggaacaagt accctatatc catctggagt
    18121 tgctcttata caaaataata taaaagaaag acaatcttct gcattagcag ttctaaccat
    18181 ttttgcttca actacagcag gattaggtcc gacacttgga ggattattgt tggacttagg
    18241 aggatggcat gctatatttc ttgtaaatat acctgttgtt ttggtttcta tatgcttagg
    18301 gtatttttta ttcccaaaag aagtaaaaga aaagaaatct ataaaagaaa ctttaaaaaa
    18361 tgttttgtca agattagata ttattggcat acttctattt tcaattgcaa tgatttttat
    18421 attactattt ttgctatcta tgaaggagtc ttttaacttg gaacaacttg tatttggtat
    18481 aatactgatg tgtcttttta tttggcatga attgagaaca aaattgccat ttatagatat
    18541 taagcttttt gtaagtaatc ctaaattgtc aaaagtttac ttgcaattta ttatattaaa
    18601 tttctttaat tatattttgt tttttggtct tcctagttat tttcaagatg ccttacatta
    18661 tagtgcaaag tcgactggat tatttatgct atttatgtct ggtataggta tatttatatc
    18721 tccattaact ggaaaatgga tagataaatc tggaactaga tttcctcttg ttacatcatc
    18781 aatctttatg tttattagtg cagtacttct ttcagtagtt tttatccata tacctgtaat
    18841 aggaaagggt attatattat cattagcagg tattagttat ggagttggta atgtagccct
    18901 gcaatcctct atgtttgaag aaagtcctcg tgacagtata gggacagctt ctggtctgtt
    18961 tcaaactagt cgttatttag gttctatttt agcaactgtt ttacttggaa tggtatttga
    19021 agtaaatata acatcagaac aatttcagat attaggttat gttatggtgg tcttaggtac
    19081 aataagtttt ttattaaatt ttatattaaa gaaagaatta agaaaagtag aatagtggat
    19141 ttatggattt aataagtcta ataactgata agtttaaaaa tattgtttgt aaaacataac
    19201 tggtttgata tttattaatt ctaagttttg aagcatagag tactcgataa gtatacaata
    19261 ttttgaatta aaattaagca gaaatttgaa ctagtctttc aaatctctgc ttaatttagt
    19321 agttaaattt ttttatagag aaaataagat ttgacactta gataaaaaat cattgcattt
    19381 tcaaaattct ttaaatcaaa cccaagtatc tcattaatct tttccaatct atatactagc
    19441 gtgtttctat gtaaaaacat actagtagat gtcttagaaa tatttaaatc attatttata
    19501 aattttattc ctgtatatag aagt
//
LOCUS       DAGSHT010000055        19072 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGSHT010000055 DAGSHT010000000
VERSION     DAGSHT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.056"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(245..1444)
                     /gene="coaBC"
                     /locus_tag="MJB72_001742"
     CDS             complement(245..1444)
                     /gene="coaBC"
                     /locus_tag="MJB72_001742"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBY8262856.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(1449..1715)
                     /locus_tag="MJB72_001743"
     CDS             complement(1449..1715)
                     /locus_tag="MJB72_001743"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBY8262857.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(1718..2335)
                     /gene="gmk"
                     /locus_tag="MJB72_001744"
     CDS             complement(1718..2335)
                     /gene="gmk"
                     /locus_tag="MJB72_001744"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBY8262858.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(2365..3246)
                     /locus_tag="MJB72_001745"
     CDS             complement(2365..3246)
                     /locus_tag="MJB72_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBY8262859.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(3250..4080)
                     /locus_tag="MJB72_001746"
     CDS             complement(3250..4080)
                     /locus_tag="MJB72_001746"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBY8262860.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(4367..5401)
                     /gene="ltaE"
                     /locus_tag="MJB72_001747"
     CDS             complement(4367..5401)
                     /gene="ltaE"
                     /locus_tag="MJB72_001747"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBY8262861.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            5697..7472
                     /locus_tag="MJB72_001748"
     CDS             5697..7472
                     /locus_tag="MJB72_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBY8262862.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(7687..9339)
                     /gene="abc-f"
                     /locus_tag="MJB72_001749"
     CDS             complement(7687..9339)
                     /gene="abc-f"
                     /locus_tag="MJB72_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBY8262863.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(9638..10849)
                     /locus_tag="MJB72_001750"
     CDS             complement(9638..10849)
                     /locus_tag="MJB72_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBY8262864.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIASITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(11133..11666)
                     /gene="pyrR"
                     /locus_tag="MJB72_001751"
     CDS             complement(11133..11666)
                     /gene="pyrR"
                     /locus_tag="MJB72_001751"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBY8262865.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(12026..12973)
                     /locus_tag="MJB72_001752"
     CDS             complement(12026..12973)
                     /locus_tag="MJB72_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY8262866.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(12942..13388)
                     /gene="lspA"
                     /locus_tag="MJB72_001753"
     CDS             complement(12942..13388)
                     /gene="lspA"
                     /locus_tag="MJB72_001753"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBY8262867.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(13698..14477)
                     /locus_tag="MJB72_001754"
     CDS             complement(13698..14477)
                     /locus_tag="MJB72_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY8262868.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(14532..15014)
                     /locus_tag="MJB72_001755"
     CDS             complement(14532..15014)
                     /locus_tag="MJB72_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBY8262869.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(15280..16701)
                     /locus_tag="MJB72_001756"
     CDS             complement(15280..16701)
                     /locus_tag="MJB72_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBY8262870.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(16847..17617)
                     /locus_tag="MJB72_001757"
     CDS             complement(16847..17617)
                     /locus_tag="MJB72_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8262871.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(17622..18824)
                     /locus_tag="MJB72_001758"
     CDS             complement(17622..18824)
                     /locus_tag="MJB72_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY8262872.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         6262 a         3364 c         1948 g         7498 t
ORIGIN      
        1 gttcatattt taatatactt tacaagatat taaaatctca gaatttataa gcaatttcaa
       61 gttattaata ttaactttat tttaataatt aactattaat tatattttat actttatgac
      121 aaaataaatg ctgaaaacta caatttaact gacatttgcg agtttttact cgctaaaatt
      181 aaattgcagc tttcagccac tttagctgtg ataaaataca caaatatcat atttaaaaaa
      241 ggtcttattt ctcaagcaac gctttaactt tatctaaaat aacattggct acttcttctt
      301 ttttcatctt aggatactca gtaatatttc cctctacatc tattattttt acaatattag
      361 tatcagctct aaaaccagca ccttctttag ttaaatcatt tgccacaata aaatctaaat
      421 ttttcttttt tatttttcct ttggcatgtt ctataaggtc attagtttca gctgcaaatc
      481 caattagtat cttatcttct tttatttttc ctatttcata agcaatatca taatttctac
      541 ttagttctat tgataaatca tcattcttct ttttaatttt tttgtcagaa taagtttttg
      601 gtttataatc tgcaactgct gcactttgaa ttactatttt attttcactt aaatttgata
      661 ctactgcttc atacaaatct tgtgctgact ctatttgaac aaatttttta agattttgtg
      721 gaatagaaag atttgtagga cctgatatta atgttacatc agcacctcta gctatagctt
      781 ctcttgctat agaatatccc attttaccag aagacctatt agttaaatat cttactgggt
      841 ctatactctc tacagttgga ccagctgtaa ctatcatact ttgacctttt aaatcttgct
      901 ctttagtaag attagtcaaa acttcctcta ctattatatc tggattagct agtttacctg
      961 ttccaatatc tccacatgca agccttccac tttccggttc aataaattta tatccaaagc
     1021 tctttaaagt ttgtatattt ttttgaacaa ttggattttc atacatatta gtattcatag
     1081 ctggtgctat aagaacctgt gcttttgttg ccataacagt agttgtcaac atatcgtcag
     1141 ctataccatt tgcaatcttg cctataacat ttgcacttgc tggcactatt aagaataaat
     1201 ctgctttttt tgctaatgat atatgttcaa cttcccaagt ttttggttct tcaaacatat
     1261 cttctactac ataattttga ctcagcgatt gaaatgtaag tggtttaaca aattctgttg
     1321 ctgattttgt cattataaca tggatatttg catttagttt ttttaactta cttacaacat
     1381 cacaagcctt atatgcagct ataccgccac ttatgccaat tacaacagtt ttatctttta
     1441 gcatgacctt actcctcttc tgaaatttgt tggtgttgct cagcatgatg tctagcttct
     1501 tctatctcta tttcctcttc tgtcaataat ctatatgtta tttttccact tgcaacttct
     1561 ttagttgcta cacatacagg cttttcctta gttttatttg aaacatatgg ttcttctcca
     1621 tcaattaatt ttcttgctct ctttgaaaca gtccctacta aatagtatct gttgtctatt
     1681 ttttctagta cctcattgat agatggtttt aacataatta taattcctcc ttaaatttct
     1741 ctattataat atttttatat ctgctaactt tattcttttc agatgatatt ataccttcta
     1801 tttctttagc agatgttttt acatcttcat taaatataaa ataatcataa tccttaatca
     1861 ttttaatttc ttcatatgca cagctaaatc tcttctctat ttcagcttct gtttctgtac
     1921 ctcttccaac aattctattt tttaattctt taagtgatgg tggtagtaca aatataaata
     1981 ctccctctgg gcaaacttct tttatttgtt ttgctccttg catttcaatc tccagtaata
     2041 catcttgtcc tttttctaaa cattccatta ttgccgcttt tggagttcca taaaaattat
     2101 cataaatttg agcatactcc aaaaactcac ctttttctat catctcttca aacttttcct
     2161 tacttatgaa gaaataattt actccatcaa cttctccagt tctgggtttt cttgtagtag
     2221 ctgatacaga aagttttatt gtatcatttt ctttaagtag ttctttgcaa atagtacctt
     2281 ttcctgcacc tgatggtcct gatacaacta gcaataatcc ttttttcaca aacatttaag
     2341 cattcccctt ctaatattgt attcttactc tatattttgt atttgttctc ttattttttc
     2401 caattcgctt tttacttcta ctactaaatt agttatattt atatcagatg acttcgaacc
     2461 tatagtgtta gtttctctat tcatctcttg tattaagaaa tctatttttc tacctataga
     2521 gtcgtcttta aatatagtat ttttaagttg ccctatatga cttttaaatc taactatttc
     2581 ttctgttata ctgcttttat ctgcatatat tgcaacttct tgtgctaatc tattctcatc
     2641 tattatactt gggtcatcca gtaattcact tattctcaaa ttcaattttt ctctataatc
     2701 ttctacaaca ctacttgaat atttttcaat ctcttctata tgatttttta ataaatcaca
     2761 tctcatggca atatcttcag ctaatttttt accttcttca cttctcattt ctcttaattt
     2821 gattaatgca tcttcaactg cttgattcaa cattgaccac aataaatctt catcttcttc
     2881 cttttcttct attttcacaa tatcaggaaa ctttgcaaca ttcataacgc taatatcatc
     2941 tataatatca aatttatttt ttatttcttt tagtatgtct atatactgag atgctaatcc
     3001 ctcatcaaaa tttaagttta catcttcttt gcctaataag tcaaacttta tgtataaatc
     3061 tactcttcct ctttttacat aattttttac taaatttcta actttatcct ctaaaaatga
     3121 aatttttctt ggaagacgta tgtttatatc tgaatactta tgatttatcg ttttacactc
     3181 tactaaaaag taataattat catccttgta ttctcctctt ccaaaaccag tcatacttat
     3241 agccattaat taaacctcca aaataccttc acatattttt actgctggac ctgtcatata
     3301 agagaaatct cctacatcta ttttaactgt tccaccttga gacgttacat ttacactact
     3361 ttctaccata ttaagtaaat tgcacactat tgctgatgca gtcataccag taccacaacc
     3421 taaagtatat ccacaacctc tttcccaagt ataaacatgt atattatact tatcttctac
     3481 ctttacaaaa tttacattcg ttttttcagg aaatatttta ttattttcaa tctcttttcc
     3541 atatttacaa acatcatcta catctaattc atctactata actactgtat gaggaacccc
     3601 catcaacata gaagtaactt taagctccct atctaaaacc tttatacttt cttgcacaaa
     3661 acgttcttta tttgtaagta caggaacgtc cttagctata aaacttcctt ttcccatatt
     3721 gactcttata gagtttattt ctctattagt aagatttact tttatatctt ttataccatc
     3781 taatgtatct actgtaaaac tttctttttt aactatacaa ttatcataaa caaatttaca
     3841 aaaacatcta aggccatttc cacacatagc tgctcttgaa ccatcagaat tataatatac
     3901 catttctaca tctgcaactt ttgaatcttt cacaactaac aatccatccg ctcctacaga
     3961 aaatcttcta tggcatactt tttttgctaa agcagtataa tcagatggat ttatctggtc
     4021 gaatcttcca tctattgcta taaaatcatt tcctattcca tgtagtttcc aaaatttcat
     4081 aagtatcgct ccttcgtaaa ttaacactta aactattata acataaaaaa attcccataa
     4141 acttataatt tatgggaaaa gttttgtaat gacaactata atttgaggat gtgagggaga
     4201 gttgccatta caattagggt aattatatta tgtttttaag ctagttcttt ctatgtttat
     4261 atattaatat ttctatatga attttatgta tgttttattt aaactgtcta tgaatttttt
     4321 aattaaacat ctggtaacta gctataatcc ttctagtaga tatctattaa catacaccta
     4381 ttaattcttt aagtgttttt aaaacatagt ctaaatcttc cttagtaata tctacatgag
     4441 ttacaaatct atattcacca ttttccattt tattaatttt tatatttctt tcaaaaaatt
     4501 cttttattaa aacatcctct tttataatat cttcactcat cttaaaaaat accatactta
     4561 tatctggatt aacattgttt acttcaatgc catcaatttt atttaattca caagctaaat
     4621 attttgcatt ttcatggtct tcattcaatc ttaaagtcat tttttcaatg gcaattatac
     4681 cagctgctgc aactatacct acttgtctca ttcctccacc cataactttt tgctttcttc
     4741 tagcttttgc tataaattct ttatttccag caagtataga acccattggt gcagctaaac
     4801 cttttgacac acagaacata acactatcac aacactttgc tatttcttta gcttctacac
     4861 ccaaagttaa tgctgcgtta aataatcttg ctccatctaa atgtactggt aagttatgtt
     4921 tctcagctat ttttttaatt tctcttaaat tatcaagact cacaactgtt ccacctgaat
     4981 atgcattttc tacacatata agccctgtct gtggttcatg tatgtcatct cttctaattg
     5041 ccttttcaac aagttcagga tttagagctc cactttcgtc ttcatctata gttctaagct
     5101 gaactccagc tattacagca ggtgccccaa cctcatgagt cacaatgtga taacctttac
     5161 caataattat ttcttgccct cttaggcaat gagtaaatag agctaattga ttcccaaaag
     5221 ttccacttgg aaggaaaata gcatcttctt ttccaacttt agctgctgct aattcttcaa
     5281 gtttatttac agtagggtca tctctaaaga ttgaatctcc aacaggagcc tttgccatag
     5341 cttctctcat ttcgtcagta ggcatagtaa cagtatcact tcttaaatcg ataaatctca
     5401 ttttttcaac cttctttcat atttatatac tatagaacct taaaattgat tttttctgta
     5461 aagttcatat actttcatag cttatactct acacaataat tatactatat ttactttata
     5521 tttaatctac tttaaattat taattttaaa aataaagttt cctctctaat aaatataaat
     5581 aattatacaa tttaaatatt aatctctatt gtttaaattc cccctagtca caaagcgtat
     5641 attatgtgta taattaattt actgactcat tttggtcaaa gaaaggggtg ctataaatgg
     5701 cattagatgg attagttata cattcaatag tcgatgaatt atcatcaaaa cttacaggtg
     5761 gaaaaataga taagatacat caacctgaag atgatgaggt aatttttaat ataagaaata
     5821 ataaagaaaa ttttagattg gttttaagtg caagtgcctc aaatcctaga gtttatctta
     5881 caagtaatta tcaaaaagaa aaccctctaa aagcgcctat gttttgtatg ttacttagaa
     5941 aatacataca aggtggaaat atagtagaaa tttctcaaat aggttttgaa agaataataa
     6001 aaataagtgt tgaatcttta gatgaattaa aagaaaagac agttaaaaat attatgattg
     6061 aaattatggg aagacatagc aacataataa taactcatgg tgaagaaaat aaaattatag
     6121 attctataaa aagagtccct tttagcataa gtagagttcg tcaagtttta cctggtcatg
     6181 attattcatt gccaccagaa caaaacaaac tcaaccctct tgatgatata tctaaggatt
     6241 tgtttattaa aaacctagag gaattggaag gacctatctt taagtctata tattctaaat
     6301 ttctaggtat aagccctatt atagctaaag aaatttgcta cagagcaggt gtaaatcaaa
     6361 atgctatcat aaaggatata tctgatgaac aatttgattc tttacacaaa gtattttgta
     6421 atttattcaa tgatataaat tctaacaaat actctccttg cattattata gataaaaagg
     6481 tggataaggt agttgatttc agttgtataa accttacatt atttagcgat ttatcatata
     6541 taaataagga tagtatgtct cgtattctgg aagatttcta tagaactaag gatattaaag
     6601 atagaattaa tcaacgttca tctgatttaa agaagagtat atctgttaag cttgatagac
     6661 tttataataa acttaaaaaa caagaagaag aattatcaga atccgaaaat gcagacattt
     6721 ataagataaa aggagaattg attacttcat atatttatat ggtagaaaaa ggtatggaaa
     6781 gtattgaagt tgctaacttc tatgatgaaa attgtaatga tgtaacaata gaattaaata
     6841 aaaacttgac accatctgaa aatgctcaaa aatactttaa aaagtataac aaaatgaaac
     6901 atgctaaagt cgagatatct catcaaattt ccttaaataa ggaagaaata gattacttag
     6961 aaaacataat tttaagtatt gaaaactgtg aaaatttagc tgaattacaa gatataaaag
     7021 aagaacttgc taaggttgga tacatcaaga ctcaaaagaa aaacagtaaa aaggatacta
     7081 tcccatctac aaaacctcat gaatttgttt cttctgatgg atttaaaata ttggttggta
     7141 aaaacaataa gcaaaatgac tatcttacac taagacttgc tgataatgat gacttatgga
     7201 tgcatacaaa gaatattcct ggttctcatg taataataaa gtgtgctggt aaggaagtcc
     7261 ctgataatac tgtatttgaa ggagctatgc tagcagcatt ctttagtaag tcaaaacttt
     7321 cttctcaggt gcctgtagac tatactaaga gaaaaaatgt gaaaaaacct agtggttcta
     7381 aaccaggtat ggttatttat gaaactaaca gtactattta tgttactcct gaagaagaaa
     7441 cagtagccaa gcttaaggtt aaatctgaat aaattctgca ctaaatacaa aataaatgtt
     7501 agacaactac acattatcat tctaaataaa aatagtggcc ttcttataaa gtacaataat
     7561 aaggccactt tctaattagc ttacttttaa ttttctaaca aaatactttt ctgttttaat
     7621 aactatactt atattcccaa caaatatttt cagtcttctt atatagatgt aattaaaaag
     7681 ttaacactaa aagctatttt ttaatttatt gatttgtttt agtaattcat tatattctga
     7741 ttcatacaat tcttttttta gattatcttt ttctatagat aacatagata taacagttgt
     7801 aagtctattt tctaatacta gaagattttc ttttttgatt tggttttctc tactaggagt
     7861 ttttttattt ctgctaattg tatattcgtc ataattacaa tcaaactccc ttattttagc
     7921 atcttttatc tctataatat agttacaaat atgtgataca aatactctat catgagacac
     7981 tataagcata gttttatcag tatttatcag tgccttttcc agtgcttcca tagattttat
     8041 atctaagtaa tttgttggtt catcaaatac taaaaaatta ttatcttcca atattatttt
     8101 acatagtgct atttttacct tttctccacc actaagtact tttacttttt tgtagacatc
     8161 atcaccttta aacccaaata ggcttaagtt tatccttata aaagattcat caaatgaact
     8221 attaacttta gtattgtata gaacactctt ttcctcatct aataaacttt gattttggtc
     8281 aaaataacct acttttacct tgttatttat ttttatattg tcatttttat cagccaatat
     8341 ttcttttatc agagtacttt ttccacaccc attatcacct aataatgcta tctttttacc
     8401 tctttttatc ttaaaggaaa cattatctaa caggagctta ttttctaact ttagagtcat
     8461 atcttttacc tctactagat ttttactaat tatctccata ccatcttgaa tcttaatgtt
     8521 catttcttta gaaactttag gtttattctt tacctcaagt ttatcaattc tgctttttat
     8581 agccttcaca tttgagtcca actttttctt attagtctga ccacccattt tgtgcaacct
     8641 agcttctgaa ttacccattc tttttggtgt ttttcttatg ccatcactta aagctttttt
     8701 atataaaaga gctttttcaa gccttttttt ctctttaaca tattcttgat actcaaaatc
     8761 agctcttttt acttcttcat ctttttgcat taaataatca gtgtaattgc ctttgtaagc
     8821 ttttagtttt ccatcttcta gttctaatat agttgtacat aactcatcca gaaaacgtct
     8881 atcatgagat attaataata atgctccctc atgcctttta aacatatctt ccaaaacccc
     8941 aatgcttttt ttatctaagt ttgaagttgg ctcatcagct attatgatat ttttcttatt
     9001 actcagcgca tctgctattt ttaacttaac cttttcccct cctgataagt gttcttcaaa
     9061 tttatctgga gcatttaaaa gactcttctc cttactatga ctacatgttt caatattatt
     9121 ttcactctga cttatataag aatagctttc tgtaaggtaa gtatatcctt catcaatttc
     9181 tatttctccc aatattgctt ttaataatgt tgtttttcct tgaccatttg aaccaactag
     9241 acctattttg tcattttcta atatttcaag tttatctata tccaaaatta atttatctgc
     9301 ataatatttc tttaaattct ctacttttac taacaacata aaaaatccct ccttaacaca
     9361 cttaaatata aggagagatt ctataaatag gcataacaaa taaacgtata gactataatc
     9421 tactctcctt aatttaagca ttgcaaataa agccaccaaa ataggctttt caaaaattac
     9481 aatactttaa attataaaaa atagattata ttctcattgt cctagttacc ttgccctttc
     9541 tttcatttat ttttaatcat tatatcaaca ttaattacaa atgtctatag aaaattaact
     9601 aattttagtt attcaatata caacttattc tttaaattta ttcttcttca actttaaagt
     9661 cctgattatt taaaattctg tttaatacaa ttccaacaat tgcagctaaa ctaagaccag
     9721 ttatctttac tgtacttgtt atagggattc caatagaaat acctttatta gataaatatg
     9781 tggttccaag acctattaat aatataactg ctatgattaa tatatttatt ttactttcta
     9841 cacaagaact gtcccttata gtttttaatc ctacataagt tatcatcgaa aatagcatta
     9901 tactaattcc acccataact gaacctggta tactttgaag aaatccacca aactttccta
     9961 cacatgcaag caatatagca aatattgctg tacgtctaag tattgaaggg tcatagtttt
    10021 tagttatagc aagtactgct gtattttctc cataagttgt atttgctggt cctcctaaac
    10081 accctgcaac tatagtcgca aatccatccc caagtaatgt tctatttaat ccgggatttt
    10141 ctataaagtt tttacctact acagcaccat tagtagtcat atctcctata tgttccataa
    10201 atacagctaa tacaactgga gatataatca caattgctcc taaactgaac ttaggaactg
    10261 taaagttagg tattgcaaat aaacttgctt ctgtaatagt agttatatct actaatctta
    10321 atatcaaaca gattatatac ccagaaaaaa ctgctattaa tattcctaat tgttttataa
    10381 atcctctacc aaacttattg gttaaaattg ctattgctaa agttatagat gctattacaa
    10441 aattggctga agccatacta aaagctgttg gcaataaatt taatcctata actactatca
    10501 tagctcctgt aacttgtgca ggaaaatata atttaatttt atttacacca actattttta
    10561 ctataatcga cattataata taaattaatc ctgctactat tataccacct tgagcatatg
    10621 ctaaatctcc accatatgcc tctttagctg ctagtataac tggtataaac gcaaaacttg
    10681 aacctaaaaa ggcaggtact tttccttctg tacaaaaatg aaatatcaat gtaccaacac
    10741 cagcacagaa aattgcaact gacggattaa aacctgtcaa aatgggaact aatactgtcg
    10801 ctccaaacat ggctaataga tgttgtaaag acattatcgt ttttttcata aaaacctcca
    10861 aaattattat atataaataa atcctctgtt taattaatat cttttaatta attatcattc
    10921 cctagattct attttttaac ctctatatac ttattatata atttatcatt attttacatt
    10981 ttacgacatc aaaaactcaa ataaaggaga ataaaaacta cattaagact cacatccata
    11041 aaattataat atttattttg tgcaatctat gtaagcttta tcctcctccc tgactattca
    11101 atgctaagct tttatataat acttaaaaat aattattctt tgattgtaac tgaatcttct
    11161 ccatcaattt ctaataaact gacagatata atttcatgtc tagaagtagg tacatttttt
    11221 cctacataat ctgctcttat aggcaactct ctatgccctc tgtccactag cactgctaat
    11281 tgtattgatt taggtcttcc tatatctata atagcatcta gtgaagctct cacagttctt
    11341 ccagtgtata aaacatcatc aactaaaact actgtcttat catttacatt aaaatctaaa
    11401 taagttcctt taactacagg ttcaacatga attttttcca aatcatctct atacaaagtt
    11461 atatcaatat ctcctgtatc tacttttgtt ccttctattt gttctatttt cttagaaatt
    11521 ctatttgcaa ttggcacacc tctagtcttt attccaacta aaactagatt ttctacacct
    11581 ttatttcttt caattatttc atgacttatt ctagtaattg ctcttgctat tgccttttca
    11641 tccattaatt gagctttttc taccatatca taattattgt caaagatata atatatctat
    11701 ttgacaaatc tactcctttc ttttaatttt ttataaattt atttttattt aaaatttcaa
    11761 tttaaacaac aaaaaaactt ctcatcacaa gacaagaagc tcttatataa aaaatataca
    11821 gtgcaattta gcaattgtct tacgatatct ctgtatcgtt taaagataat tttttaatct
    11881 atcaaatcaa attatatatt tatatttcaa atttttcaag cttttttaaa caaaatactt
    11941 taatataaga tattttattt aaatttcact aaatttattt acttatataa aaacaaaaaa
    12001 tgatttataa gtattataca aatggttatt ttatcttttt aataacttcc ttaaaatact
    12061 caggtaattc tgaattaaat tcgatatatt ccttagtagt aggatgtata aatcccaact
    12121 tcttagcgtg caaagtttgt ccttttaatt taaatttagt ttcttttgga ccatatacat
    12181 aatcacctaa aagtgggtga tttatagaca aagcatgaac tcttatttga tgagttctac
    12241 cagtttcaag cctagctctc atatgtgtaa acttatcaaa tctttctaca acttcaaagt
    12301 gagtgattgc attttttcca tcttttacta cagccatttt taatctatcc ttaggatttc
    12361 tccctatagg tttatcaaca gttattttat cttctttaac cacaccatag catataaatt
    12421 catactccct agttatagta tgttccttta actgttctgc tagaaaatta tgtgcataat
    12481 tgttcttagc tatcataaga agaccagaag tatctttgtc tattctatga actataccag
    12541 gtctaataac tccatttatt gatgacaact tatctttaca atgatataaa atcccgttta
    12601 ctaacgtatt ttcatagttt ccaggagcag gatggacaac catatcctga ggtttattta
    12661 ttattaagac atcatcatct tcatatacaa tatctattgg aatatcttga ggtaaaactt
    12721 ctaatacttt aggttctggt atctctataa caatattatc ttcctcttta accagatatc
    12781 tagctttttc tactttatta ttaaccttta cttttccatc ttttataatt ttttgtatat
    12841 aacttcttga catatcacct aattgttctg ctagataaat atcaagtcta acttcttctt
    12901 cctcttcaag tactaaaaac tcttttactt catccattat atcacctact tttactttca
    12961 aaaaataaaa catatataca taaaaacaca gttcctacaa ctacaaatac atcagctata
    13021 ttgaatacat attcccatat aattctaaag tcgaagtaat ctactacaaa acctaatcgt
    13081 actctatcaa ttagatttcc caatgcacca gatataatta atataattcc caaccttcca
    13141 aatatatgta ctttccttgt attaagatag tatagtccaa atactgttgc aagtaatgca
    13201 acaattataa atatccattg attattttgt aataatccaa atgctgcacc tctattttcg
    13261 acataagtta aatgaaatac attatttatt attggtattg ttgatacatc caccaaatta
    13321 ttcaataccc atattttaga cagttggtct aaacctatga gtagaattat tattaatata
    13381 tatagcaatg atttttcccc ctataaatta tttaaaaacc aattttattt gaagcacact
    13441 attattatta tacaaaaaaa atgctctaaa gagcaacttt attaaaattg tattttctta
    13501 ataaagctag aacaaaattt aaaatagtac tacttttttt atattgtaga tttctacata
    13561 tttaaaatca tatcattatg tataaaaaat ttaaaaatac catactaatc aatatttttt
    13621 gtgtatttta tagattaaag attaatgatg tctgtaattt aaacttacaa tcaccactaa
    13681 tcttcaaaca aatattttta cttttgcact tgcttatttt tatctaaatg ttgtataagt
    13741 tcagatactg ttacgaattt atatcctctc tcttttaatt taggaattac tacttctaaa
    13801 gcttgtatag tttgtgattt atcatttctt ttagtgttat aatcatgtaa aagtattata
    13861 ttaccatttt ggacctttgt aaggatagta tttataattg catctactcc agggttagac
    13921 caatctctag ggtctaagtt tgaccaaaga attatattca tgcctttaga aactataata
    13981 tcacagacac ttttactcat ggctctatat ggaggtctaa atatctttgg ttctactcct
    14041 attacacttt ttataatatt ttgcgtatct gttatttctt tttcaacact actataacta
    14101 ttttttgcaa catttatatg tgtaaatgta tgattcccta tttcatgccc ctcatcatat
    14161 tgtcttttta ataattcttt gttatattct actttttctc caactacaaa aaaagttgct
    14221 tttacatcat ttttctttaa tatatcaagt acttgtggtg taaaatcctc atctggacca
    14281 tcatcaaaag tcaatgctaa aattttttca tcctcatttc ctttttttat tattacatct
    14341 tcataatcgt cttttatatt gtcatttaaa ctatatgctt cttttgaact atcttgatta
    14401 tgtgtattat gattatatgt atatacacct actatagata ataaacatat acatataccc
    14461 catattattt tcttcatgtc attacctctt ttctaattca ttctagcaat atattttata
    14521 atgtcagttt tttaatctct caatattcca tttttattta aatcagttaa atttgttagg
    14581 tcttctgaat aaaagttaga atcttctaca tcagggtttt gcattgcatc acctaataaa
    14641 ttattttctt tctttgcaca atcattacat aaacttgtat ctggcaatat atccaatctt
    14701 tctaaatcta tttgattatg acataaatca caaatcccat atgttccatt ctctatttta
    14761 gataatgcta agtctatttg atacaatctt ccctcttcat gatttattag actgttttgc
    14821 atattttgca tatataaatc tgttcccatg tctcctatat gattatcata actagataat
    14881 tctcctgatg aatacttttc acttgtatgt ttagttgtat ctccaaataa agtattatct
    14941 ttcatatcct ctactaaatt agttaaattt tctctttctt tcaataattt atctttatat
    15001 tttttagtat ccatttttaa cacctctaat tttaatattt acatcataaa aatagtatgt
    15061 ggaaaagtaa attaaacata caataaaaat aagatttgat tcgaaaaatg gaatgtctca
    15121 aaatgaaatt tttagttcat ttaaccctct tttttatatg aaatcaaata tatttttatc
    15181 ctttcagcaa taaaaaagag acttatctct attttgagat agtctcttct ttgaacataa
    15241 tttaaataca attttttaat ttttatagtt attattttac taacttgcca atttatgatt
    15301 attttgttct ttaatatgtt ttgctttttt aaagaataaa agaccaagaa ttattgctac
    15361 aataacacct attccattag atatattaac tggtagtcta aatccttctt ttgcttgcat
    15421 tatataacta aatgttacta cagtcataaa aagtgctggt aatgtagtta tataatggtt
    15481 tttatttgct ttgactaaat atgcagaacc agtccacaac attatcattg caagtgtttg
    15541 atttgcccaa gagaaatatc tccaaattat attaaaatct ataaatgtaa gagcaacacc
    15601 aactgcgaat aatggtaaag ctattaaaaa tctatttttg actgcatctt gattataatt
    15661 cattgcatct gctattgtca gcctagcact tctaaatgct gtatctccag aagttatagg
    15721 acatgccaca actccaagta tagctaaagc tcctccaata ggccccatta atccaacaga
    15781 tactgtattt actactgttg ctgctccacc atcattaaat atcacattaa gttgaggtat
    15841 gccatggaaa tatgacatgg ctgctgctgc ccaaactaaa gctattatac cttctgcaac
    15901 cattgaccca taaaatactg gtcttgcctc actctctttc tcaacacatc tcgccataag
    15961 tggtgattga gttgcatgaa aacctgatat tgctccacat gctattgtta taaataagta
    16021 tgggaataaa ggtgttccat ttggattcat attttgtaaa tttatatttg gtatgttata
    16081 cccctttatt attaatcctc cagctacacc tactgccata attaataatg ctgctccaaa
    16141 taaaggatat attttaccaa tcaatttatc tattggtaaa acagtagcca taatataata
    16201 aagtattatc actccaaccc atatttgtgc gtttacacct gtcaatgtct ttaataatcc
    16261 tgctggacct gttacaaata caactccaac caatactaaa agaataacac taaatattct
    16321 cattgctatt tttgcaccat ttcctagata aaggcctacc aactctgatg cagatgtacc
    16381 tttgtttttc attgatagat atcctgaagc aaaatcgtgt actccaccag caaagattgt
    16441 tccaaacaca atccataaaa atgctgatgg tccaaataat gctccttgta tagctccaaa
    16501 tatcggacct aaccctgcta tatttagaaa ctgaattaaa aagattctct tccatttcat
    16561 tggcacataa tctactccat ccctacaagc tatagctgga gtttgatttt tgtcattggt
    16621 tccaaaaacc ttttctacaa aagtaccata gacaaaataa cctactataa gtactactat
    16681 tgaacctaaa aagcttacca taaattcatc cccttagttt ttatataatt gctttataaa
    16741 tttttatgag tttaagaaaa ctatttccag ttcaaatagt aatacttttt tatgctaata
    16801 taaatattaa ttacattaag tgcatattta agtacatgaa atgactttat atatgcatta
    16861 acagcctaaa ttcttttact ttgcttctac ttacagttac ttcataatca ctatttttta
    16921 attttaatat gtatgtatta ttaaaccatg gtataatttc ttttactttg gttaagttta
    16981 cgatataaga cctatgtgtt ctaaaaaatg ttttatcatt tataagattt tcgacttctg
    17041 atatgccttc ttttaatata aacttttctt tctcagtata tataaatgtc tgcctttcat
    17101 tagcttcaca gtaatatata tcatcaatat ctattacaac taatttatca cctttccaaa
    17161 ggctaatttt atgtgttatt tcttctatct cctgatttac tgcttccttc ttatatttgt
    17221 ataaattact atttacatta aatagttcaa tatcactcat ctcacttttt tctaatttat
    17281 tcagcataga aataattctc tcttcagaat atggtttcaa tatataatca aatacatcca
    17341 aagaaaaagc atctactgca taactttcat atgctgttat aaaaacaatt ttaggtcttg
    17401 atttaaactg atttaatgtt tttgcaagta gcattccatc aagatgaggt atatttatat
    17461 ctaaaaaaat tacatctatt ttattttcct gaataaagtc taacacctct ataccattgg
    17521 taaattcact tactacctcg attccacttt tattttcaat aaaatatctc aattcctttc
    17581 ttgctggaaa ttcatcttcc actattatag ctctcatact tctactcctt tacataaaat
    17641 actatcaaag tcccattatc tattcttctt atattaagcc ctcttccata catcagttta
    17701 agccttaaat gtacattata aagcccaatc ttattttctg gcatattatc ttgatataca
    17761 ttatttataa tttcctcact tatacctata ccattatcct ctattgatat tctcacagta
    17821 ttttctaatg aatacggtaa cttttttatg gatattttca ctacaccagc tctaccactt
    17881 tctaaaatac catgtattat tgcattttcc actaatggtt gtataatcaa acttggaatc
    17941 tttatattta catcttctat atcataagat acagttagta attctccaaa cctagctttt
    18001 tctatctcta caaaagattt aacttgttca atctctttat ttatatcaat taagttatca
    18061 ctatattcaa gattatatct tagataagtt gataaattta ttattaactc tctagcctta
    18121 tctggactaa atcttataaa tgaagctatt gtgtttaggg aattaaacaa aaaatgtgga
    18181 ttaatttgtc tttgaagtgc ttttatttct gctttatttc tcgcttctat atctttttta
    18241 tctttctcaa tcccctctac tatagatata acaaatccta ttccaatctg cccaactatt
    18301 aaaggtaaat atattccacc gattatttga attgctaaag aatgtggata tgatattaag
    18361 tatataagta atatagtaaa actttcagaa atcatgccta ccaatattcc atacattact
    18421 ctatgttgtt ttggtatcct cttatataga aatcctgata atacacctgc tagtatactt
    18481 gataaaagac atggaacaga tgttatcccc ccaatatcca taaaatatct atgaatccct
    18541 gaaaaaactc ctgctgtaat acctaccatc ggaccaaata aaatgccacc tgatactata
    18601 gatattattc tagtgtttac tattgacccc atataattta taccactata agttccaaat
    18661 atggctaaaa aagtaaaaac taatgctata cagaccaagt cattaaaact atattcttcc
    18721 ttttgaaata tctgcttaaa tatttttaac tttgttatta gaagaaacaa tactacaata
    18781 aggcttgtct tttctattaa tgcgattaaa attgatgttt tcataatccc cctttaaaaa
    18841 ataaattttt acacactcta aatttattat ggttgttata aattacatat ttgaagtaat
    18901 cctatcacaa aactttccac atttaatctt ttaattaaat aagaaatagt tgtacttcta
    18961 ttaacaacta atactagcta gcataagctt gtaataactt gtagttatta ttaactttac
    19021 aattctagta tttaatttta tactttagaa atctcttttc ttagaaataa ta
//
LOCUS       DAGSHT010000056        18826 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGSHT010000056 DAGSHT010000000
VERSION     DAGSHT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.057"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(180..716)
                     /locus_tag="MJB72_001759"
     CDS             complement(180..716)
                     /locus_tag="MJB72_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBY8262873.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(734..2047)
                     /locus_tag="MJB72_001760"
     CDS             complement(734..2047)
                     /locus_tag="MJB72_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBY8262874.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2402..2842
                     /locus_tag="MJB72_001761"
     CDS             2402..2842
                     /locus_tag="MJB72_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY8262875.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3027..3215)
                     /locus_tag="MJB72_001762"
     CDS             complement(3027..3215)
                     /locus_tag="MJB72_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262876.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4156..4551
                     /locus_tag="MJB72_001763"
     CDS             4156..4551
                     /locus_tag="MJB72_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262877.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4594..6693
                     /locus_tag="MJB72_001764"
     CDS             4594..6693
                     /locus_tag="MJB72_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBY8262878.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6918..12128)
                     /locus_tag="MJB72_001765"
     CDS             complement(6918..12128)
                     /locus_tag="MJB72_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBY8262879.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYSKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSSKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12143..14638)
                     /locus_tag="MJB72_001766"
     CDS             complement(12143..14638)
                     /locus_tag="MJB72_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8262880.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLKYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKIEKVVYYRVELSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNIKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15316..15603)
                     /locus_tag="MJB72_001767"
     CDS             complement(15316..15603)
                     /locus_tag="MJB72_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262881.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15623..15898)
                     /locus_tag="MJB72_001768"
     CDS             complement(15623..15898)
                     /locus_tag="MJB72_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262882.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16553..16732)
                     /locus_tag="MJB72_001769"
     CDS             complement(16553..16732)
                     /locus_tag="MJB72_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262883.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17017..18483)
                     /locus_tag="MJB72_001770"
     CDS             complement(17017..18483)
                     /locus_tag="MJB72_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262884.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSRDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         6244 a         3287 c         2063 g         7232 t
ORIGIN      
        1 aacaaataaa tttatattga aatacattaa attatataat tttatttatt ctgagagtat
       61 aattataaaa aaaagaatag gatatttttc ctactctttt ttctttactt atatataata
      121 ttaaaactct ttatttttta atatatattt ttcttttaat atatctcttt tatatctctt
      181 taattttctt cttctatatc agagccaaag ggaatccatg cattaccaaa ctctttatct
      241 ttaaataaat aagctaatcc agttatttgc ttcaatctaa gaatttcatc aacttccata
      301 cccaggtgct ttgatatcca ttcatcagat tttccaattt cacaaagttc agttataatt
      361 ttactcatta aatccacatt atgagaacct cttgcacgat tgtgtcgaat agtacttgcc
      421 attctttcat caagagattt atcaattact gaaacaggaa gaagtccgtt ttctctatca
      481 tatatatcct cattttcaag cattactcta tatctatgaa aaccatctac aataatatac
      541 ttatcctttt cctcattata ataacagaca attggcattg tatacccatc ttcttttata
      601 cttttataca acaactgcat ttctggtggg gctactgcat ttggattata gtcgttagat
      661 tctatctttt cgattggcac tgcgatgatt ccatatacag gacttttata ttccatagca
      721 tcttaactcc tatctaatga attttttcat atttcttctt aatatattcc gttctaattc
      781 tctgttcttt tgtaggacca aatcccataa atctacacat atggtcattt tttaaaatac
      841 aataacacat tcttttccaa cttggtatat ctttggtaaa tttaatatca tccgtatcat
      901 caggtatatc atccaaaaat atgactctac tctttttatc ttttgtatga tttgaaactc
      961 catttaattc tattttataa ccttttctca acaattcacg tataactatt tctgacaaac
     1021 caccacctgt attctgccaa aactcaatag atgttgaaaa ttttttaata tagctattcc
     1081 ttatattttc aggtaaggta tcaagcaaaa aatatgtgaa acttttccat gtgtgtccat
     1141 atggcaattt aacactacga tatcccatag cttttgtttt gccataaata gaagtaaaat
     1201 taactccttg tactctagca accaatttca cccatacatc tgggtccaat attctgtata
     1261 aatttaaact ctcttttgct gaatcattaa aaggtgatgc cactctcatt tttcttggag
     1321 ctactccagc tttatagaat aagtcataaa ttttattata gtcatagtca aatttatagt
     1381 gtgctaacca tatatcttct atttcccaat catatatagg agatgccgtc cagacatttt
     1441 taaattcctt agttatccag cactttcctt tatatccatg ctgtttattc acaatagaac
     1501 aataacgttg tagagattct gctgctcgtg ttcctaataa gcaaacaact tttgcacctt
     1561 catgtatatc tctaatccat ctaccaaatt gttttgccaa actttcttgt ctcattttat
     1621 atttataata agtaaaagga ttattttttt gatttattat gtattctttg actggcattt
     1681 cccttaccca taactcttct ttctcatcat cccatggata ccaatacatt tcataatttc
     1741 ctacagcagt tcttgtagcc attggtaaac acacccaata tggttcaata tcttcaatat
     1801 tatcttcaaa tactttttca acatattcag tagtcaaact atattgagcc tcaaaatctt
     1861 gatgaaatac tcctattttg gaacttatat tattctttct cttataatcc aaaactaaat
     1921 ttagcagtaa accactatct tttcctccgc taaaagatac atagatataa tcaaattcat
     1981 taaaaagata agaaagtctt tcttgaaaag cttcaaatac atcggattct aaatatctct
     2041 tatccatttg aattatcccc tttcaattag aagtttataa aaccttacaa tgcaatatta
     2101 ttatgctttt ttagcagtaa tcagcataaa ttatacatta tttaaataca caaatccaac
     2161 taaatacata taattataat gtaattatac ttttgtatta taaactatat tttatcatta
     2221 ttttacattt ttttccaata tatataatta tcatattttt ctattagcta aaactaatta
     2281 ccttatattt ttacgtattg gtataataat acataaaaac ttgaataatg cataaaaact
     2341 tgacatactg ttgtcataat tactttgcta taattaaaag taaaaatgga ggtaaattct
     2401 aatgtatgaa cgtatgcttg ataaaagcaa taaaccctgt ttgtctcaaa taatagaata
     2461 tataggtaaa gatggatatg cacgattgtg taagttggaa acttacttga aaactcaata
     2521 taatttatca agagaacttc gatttccttt tggaaacaat tatggttggg gttacaaata
     2581 tagccataaa tcttttcatt tattttatgc tttttttgaa aaaggtgcat ttactgttct
     2641 gattcagata ggtgacaagc aagttccagc agtagaaaaa acgttggtag ctttgacacc
     2701 aaaagctaaa gaactctggg tcactcgtta tccttgtggc aataatggag gctggattaa
     2761 gtatcgtatt ttagaagatg cagacttact tgatattatt caatatataa gagcaaaaaa
     2821 atctccacct aaacaaaatt aacttttatt ttttgtgatt taaatatagt gtatataaat
     2881 tccattcttt ccatataaaa aaaataatat aaaaaatatc tattagtata acttatttag
     2941 tatgtcttat tctacttttt acagataatt aaaaagtttt atcataaata aatcataact
     3001 agacatccta gtaaatcaaa attaatttat tcttttttat tagaattttt gataacagtc
     3061 cctatacata tgccaattat cattccaaaa attagaccat aacacattgt gatacctaca
     3121 tttcctttag aaatacctat agcacaacct atagccattc caagtatcat gcctataaac
     3181 atacatataa taactaaatt gttgttttta ttcatagttt ttctccattc aatatatttt
     3241 aaattgccta gctcgccttc ttagcttaaa acaaagaaat ataaattaac tttctttcat
     3301 tctaatatct gcttgtataa atgtcaaaca acacatttct tgtcataacc tcctcttttt
     3361 gaagtaagta caatctccca gacgcaataa tatcaatttc ctaaaaccat ttaagtaact
     3421 tttatattta cataatattt acaatcataa actatattgt tattcattta tacatcataa
     3481 acactccctc ctaatcatta ttatacttaa aataatgttc atttataata atatatctaa
     3541 cgcactttaa ttgaaaaaac taaaactttg cctttcattc ccatatataa ataagtatac
     3601 tttttgatgc tctattacaa atcaactaaa ttaattgata taaattatca ttttctgttg
     3661 aaaataccat tcgaacatgc tatacttaat tgcataaatg gtaataaatg tcatatgatt
     3721 ttacaaatct attaataaca attattactt ttactgtatt ctaaagtttt tggactaaat
     3781 atcaagaact ttctaaaata caaagtacaa aaaatttata taagttataa atttatatga
     3841 actagataag ctcaaacaat atgttatgta taaactgtgt agtattcggt tataaattta
     3901 tataaattat ataagctaga taaagtatta cctcaactat ataagctaca tatataaatt
     3961 acatacataa gctatataag aattatttca actatataaa ctacgcatgt aaaattcatg
     4021 taaatttact aatataaatc aaataaaatt actaaactct ctaaactaat tttgtaaaac
     4081 atactctatt tttaacattt gttaccttat acataagtac aaacaaaatc taactaaatg
     4141 aaaggataat gtaaaatgaa tattttaaaa atgaaaaaaa cagggatatt tttaggagga
     4201 gtactttttg gtactgtagg tgttaagata ctttcaagtg attgtgttaa aaaaacttgc
     4261 actcgtgcaa cagcaggagc attgaaagtt aaagattctg ttcttgaaac aactacgaaa
     4321 attcaagaaa atgtagaaga tattttagct gaagcaaaag aaatcaacga gcaagaagac
     4381 ttatctacat ctaaggtaaa cgttgtacaa gatactctta aatgcgaaca agaagttctt
     4441 aaaactgaaa ctataaaaac tgtagaagat actaattcta atagcattaa atgtgaagaa
     4501 atcaaagata acgtaattga agaaaaaaat agtacttgta caacagaata aatttaatga
     4561 ttattatgat taataatatc aggaggaagt aaattgaatt tttatataaa acacgaaata
     4621 agaggtcgta tacgctttga cttacaactt ggcaaattaa acaacaagca gtccgatact
     4681 ctcttatatt atttaacttc tataaatggt attaccaaag caaaagttta cgatagaact
     4741 ggtgatgcag tcatttatta cactggtgaa agagaataca taataagaga aatcactaaa
     4801 ttcagtttta acaatagcaa aatagaaaac ctagttcctg aacataccag cagggaatta
     4861 aacaattatt atagagacaa attagcaaat aaactgctag tcaaaagtat ctccaaaata
     4921 ttttttccaa agtccttaag atttttttta attggacttc attctataaa atattttaaa
     4981 aaaggtctct cttccctgct aaataaaaaa atagaagttt ctgttttaga tgccactgct
     5041 ataggtattt ctatttttag aaaagatatc aatacggctg gttcagttat gtttcttctt
     5101 ggtatcggtg aactattaga agaatggact agaaaaaaat ctatagacga cttggctcaa
     5161 agtatgtcac taaacattga aaaagtatgg ttaagaaaag gtgacactga aatcttaatt
     5221 ccaatttcag agataaagga aggagattta gtaagtgtaa ctatgggaaa tatgattcca
     5281 ttggatggag ttatagtatc aggagaaaca atggtcaatc aggcatcact tacaggtgaa
     5341 tcccttgcag ttaacaaaaa agaaggctct tacatatatg ctggaactgt aatagagcaa
     5401 ggcaatattg ttatgtgcgt caaggaaaaa gctggtacta ctagattcca gaaaatagta
     5461 actatgattg aggaatctga aaaactaaaa tcttctgtag agagtaaatt tgaacatcta
     5521 gcagatacat tagttccata cagcttttta ggaagtatat taacctatgc aattactaga
     5581 aaccctataa aatcactctc tatattaatg gtagatttct cttgtgcatt aaaactctct
     5641 atccctattt cagtactatc agccatgaga gaatgtaaca ataacaacat tactgtaaaa
     5701 ggtggaaaat ttcttgaagg tgttgcatgt gcagatacta ttgtatttga taagacaggc
     5761 acattaacaa aagctcaacc aactgtttca gatataatta cattccaaaa ttataataag
     5821 gaggatatgt taagacttgc agcttgctta gaagaacact tcccacactc catagcaaat
     5881 gcagtagttt atgaagctga aaaacaaggg cttagtcaca aagaaatgca tacagaagta
     5941 gaatatatta ttgctcatgg catttcaaca aaagttgatg ataaaaaggt tgtaataggt
     6001 agcaaacatt ttgtatttga agatgagaag tgtactatcc cagacggtga ggaggataag
     6061 tacaataatc tatcagatga gtattcacac ctatttatgg caatatcagg taaattatca
     6121 gcagttattt gcataaatga ccctttacga aaagaagcaa aatatgtaat ttcaaattta
     6181 agagaatgcg gaataaaaaa gattgtgatg atgactggag atagcgaaaa aacagctaaa
     6241 tctatagcaa gtaaagttgg tgttgatgaa tattattcag aagtcttacc agaagacaaa
     6301 gccaattttg ttaaaagtga aaaactcaaa ggtcgcaaag taattatgat tggtgatgga
     6361 atcaatgatt ctcctgctat ttcagaatca gatgtcggaa ttgcaatgag tgaaggtgct
     6421 gaaatagcaa gagagatttc agatattacc atatcagcag ataatttgaa taatttaatc
     6481 gttttaaagc agattagtaa taagcttatg aaaagagttg atttaagttc taagtttatt
     6541 atcggcttta atttaggttt gattttactt ggtgtaggtg gatttgttag accaagtaca
     6601 tctgcctttt tacacaacgc atctactgtc ggaataagtt taaacagtat gacaaattta
     6661 ttggaaaaca ccaatactta taactaccat taatctccat tacataaaac atatgatata
     6721 aaaaatttat aattcatctt tttaaaaaaa tatgtcttaa aattagcttt tttctaatca
     6781 aaattatata aagcatactt tacaaatttt aaactcaact tatttctatt ttttacaaaa
     6841 aaggagatga ttcaaatttt cattttgaaa catccccttt tgtaaatttc tttaaaattt
     6901 atatattaat tatattttca accaataatt gtattttcaa ccaatctttc ttcatagcat
     6961 tttaaatcat tttgctttag ataattaaat tcagactcta cataaatacc acagcctacc
     7021 aattcaattg tcaaattagg tatattttcc tttagtatac tagtctgctc ataaataatt
     7081 ctttccaata aatcaacttg taatatagaa aaatgcttca taatctcccc atcactatct
     7141 atatctttct tgatttcata ccaactcttt gacaaaattc ccttcaaatc aatataccct
     7201 agactcctca atatatctat actatcgctc catgcatact tactaggttt atcaaagtat
     7261 gactctagtt tcttcttctg ctcatttgta agtgaatcat aagaaagctt cctattaaaa
     7321 tgtgccttca aagaaatctt agccatagaa tatggacttc taccagagtt taatcctgca
     7381 tttttgaact tttcaattcc tttatcttct actgaagacc atattgagtt tattgaatct
     7441 gaatcattgt atttaacatc aagaccatgt gtaatattta tatagttttg tggaaataca
     7501 tattgataat agacatcttt tttatcatca tatagagaga agtatccact atcatataaa
     7561 tgaatatgca cctttacttt taaactatcc tctaaaatat ttcgtctaag gttcgggata
     7621 tttgcataca tggttacata ttttatggca tcaaagtttt tatttaatgg aacttcaaaa
     7681 ctaatttcat ctcctgtcaa aagctttttc acttcttctt cattacaatc atatagagaa
     7741 tatgtatttt caaatcccac acgtgatttc ccatctaaac tgcctgttaa tgctaccttc
     7801 aaaatatggt tatctgtttt ttgccattgg taagcatctt ggtcaagaaa ctctctaatc
     7861 tgtctatttt gctctttcgc ctgatttata acctcgtttt catcttctgt aatccctaca
     7921 tggcatctat ttaatatatt tactatattg cttccaaatg ggaatgtcca tagttgaaaa
     7981 gctgctgcct caacctgaca caagtatcta acctgattta attcaatttg atggataagt
     8041 tttttctgat attcattcat ctgattacca ctatctgaca tttgataaat caattctccc
     8101 attgcaagat tatctttttt cttatcaatc ttatcagtaa tgataatttg agtttccatc
     8161 atatcctgta cttttttaaa tattatagaa atctcctctg aattttccat ttttaagctt
     8221 ccaaacttcg tgcttaattc agttaaaact tccttcatct ttaatttact tatctcattt
     8281 tcaaaaatat cttgattttt aactgtacta tcgaattttt ctacaaatgg tataacagat
     8341 tctttcaaag ttttatcttt aaattccacc atttgagtat tcaattcatc taatttctta
     8401 atattatttt ctttatcttt aattgcctct ttaactacct ctattttact ctcaaaacta
     8461 gattttacat taagacaatt attaacacca ctaatcgcag tatttataag agaacaagca
     8521 gaacttccaa tactcaatgc atttttaatt ttagatgtct tttgctgtct ttcaatagct
     8581 tgtttacttt tctctagtgc agctaactta tctttttgct gtttattatt gtaatttgcc
     8641 tccaacatac ctatttcttt tttctggatt tttgtgtaaa tttccttata ctcgctttca
     8701 cttaacccct cttttgctct ttgaatatcg tcagctttgt ctgcatagtt atcatcatct
     8761 atagacttaa ctataatctc cttaatttgg tcaaatttag ttcttctttc ttcatcaaca
     8821 cctgccaaat tataagtcat tttatctaaa gcattttttc tatcttcact tagctgttct
     8881 ccaagtacaa atctcatacc atcagaatcc aatttaaaat ctgaaaaatt aaccatcctg
     8941 ttagtccatt tgtattcctc ttctttagat gaaagatttt ccttcaaaga ttttgcactg
     9001 tcaaatgccc ctagacaagc ttgaccaaca ccactaaatg cagtcagtaa actcaatgcg
     9061 ctttgtattc cagaaagaat taactgtgtt ttcatttgtt cctcaagttt tgcctgtttc
     9121 ttttttagtt cctctatgtc ttcttgttca tcttctttca tctttataat ttcttgaatc
     9181 aacaggttca tttttgcatt aacagaaagc attttattct caagttcatt ttttaaaacc
     9241 tctatttgtt gatttccaga ttcaatttga gttaggatac tttccttata tgtattgaaa
     9301 tatccttctt ttaactcatc tttcttccct tgttccaatt ctcttaaggt cttatccaga
     9361 taatctgtca aagaacgaat atctgtaaca cgatttcctc caatttcact ttcttcaatc
     9421 atctgcacat aaaatgctgt cttctgctca agtgcaaata aaatcatact ccattcgcta
     9481 aaattctgat tttttcctac aaaagattct attctatcag actgcatcaa tttatttata
     9541 aggtattcat agatttcctt tgcttcttta taaaattctt tctttgtatt catatcatcc
     9601 agagaaagta ctgctttatt tttcttctca aaactatagt acttctgaag tatttctcta
     9661 tttaattgca tctctatttt ctcaactgca tgaaaaattt cataaatatg tgaaagttct
     9721 aaaatatcag cttctaaaat tcctactgca cacaacagtc taagatttct ataaccttta
     9781 attccagaaa aaccaaatct ctcgaaaaat tcatagaaga atgtatctat tgacaaagaa
     9841 tactcctgct ttaacaattc cttttctttt agtgcatctc cagagtgacc agcttttccc
     9901 tctgctccgg ctgaacctga atcttcacgt ttgctaattg tccagtcctt atgttcttca
     9961 taaagagtat ttaatttctc tccatcatct gctgcttgct tctgatttaa agcatgatta
    10021 aacacatatc tatcaaaata atagtagttt ctatcactat tatccattgg aggagaggta
    10081 cctatatatc ttattgtttc ttcatatata cctctaattc caccaagacc tcctgcacca
    10141 ccagctcctc cagagcctcc ttttcctgct aaaccatgat ttccatcttt tttacttata
    10201 gcacttttta ataaaggagc ttttccaagg agataaacct gtccagactt gcttccaaga
    10261 cctgcatttc caccattacc tcctgtgcct ccagttccac cttgttttcc tttttctgct
    10321 tttacaacat agtattttgt acctttaaca ttagcaaatt cttttgactc ttttttagaa
    10381 cctattttag catttgcccc agcttctcca gttatccctg tatctccttt ttttccatct
    10441 tgtccatttc caccacgtcc accatctacg taaagtttaa gttctcctct tttttctatt
    10501 tctttaaaag ataatatgat atcacctgaa gtacctgcat tattgccatt tcttccattc
    10561 attccgtcgt aaatagaact ttctccaacc aatttttctg catccttacc agatacgttg
    10621 attgaataag tcccttctgg tatatgtaat ttttctgtaa gtagtacaat attcttacct
    10681 gagaagactt ctttctccat attacagtca attttcaact cacaatctcc atacatatat
    10741 atagatgaca aaatattaag ttgataacca gatattattt tagtcaaatc cgataaattc
    10801 actttaggta taggaatata tatagaaagt acattttctt tctcatatga ttttatatat
    10861 gtacaacttt gaatcttctc tatcctatac tctgcatctt caagcttttt ggcttcttct
    10921 tccctatatt cttcttctgc tatccaatat ttgatatctt ttaatgtatc ttctaaagta
    10981 ttttttagca atgattttaa caactcactt agaccaacaa tatgagtctc actacagtaa
    11041 ctatttaact ctaaaagaag acgtgtttct tctaatatat tcactttctg tacgccatca
    11101 ccatatgaaa gttcttgtac aaactccata agctctttca tattattagt cctactacat
    11161 aaatctgtat acatcttatc taattgaatc tttttatctt ttagactatt tatagaagat
    11221 agaatggaaa tagaatcttc catacctttt ttaacacttt ctatataagg aactaaaagt
    11281 ttttctaggt ctttataggt tacatcaaca gctttttgta tttctatttt acaagaagca
    11341 gtaagcatca atcccaaatc tgttttttca aatgtttcag gtagattttt catttcattc
    11401 aacaactttt ctggctgttt atctagtgga ttacataatt taatagatac accatctgat
    11461 aatgctacat ttaataagta cactaattcc ttacaaccgt cgtaatcttt ctcttgacta
    11521 tctaagtctt cttttgcttt ttccaatata tttcttattt tttcttctgt tacattacca
    11581 attagtgttt taggtatttt attaataaga aagaagaaat tttctttata ctttgaataa
    11641 tcaccaagca tatctttcaa atttttgcct gtttctatca ataatgtatg tctagagtcc
    11701 agtagggatt gatagtctaa aataactacc atccctttca cctttgaaga tgtggaaata
    11761 gtttccctta caagcatagc accagcaatc atatactctg ctccacggtt gtcaaaaaaa
    11821 cctgggcaat cacataatga aaaatctcta tttgcaatat ctacaacttc tggacataaa
    11881 gtcttagaat atacaccatt tccaatttcg acagctgaat cttttgcagt tatatatatt
    11941 tgtccagtct cttcatccgt tttttcttcc atcacacagc cttttaaata atttatacaa
    12001 gtactctttc caacgcctga agcacctagg aataacaaaa ttgacttttc ttttaagtca
    12061 tattttctaa gctcgtctct tccttcttcc aaaaggttta cgatttcact ataaagttta
    12121 tttgccatac atccatccct ttctatattt cggaacaata gccttgatat ttttctttaa
    12181 taagattctc ttctaccttt gaaacagctg ttgataactt aatgtttgga gcattaaatc
    12241 ctccattgtc tcctctatct ccatattcag cgtattctct tgctggtcct gttttacctt
    12301 cattccagct tccaccacta agttctacac gataataaac tactttttct atcttataat
    12361 cccaacttgt aaaatatcct gtatagtata cagtcttatc tataccactt ggagaaaatg
    12421 aaagtgtatt atatccgtga tatccaccac ttcctccata tccacctaaa cctggtctac
    12481 catttggacc tgcttctcca tctttcactg ttatatcaca atgattaatt attctatttt
    12541 tttgttcctc tgaatctgaa atgaagtaaa tattaccttt cttaccagca tttccacctc
    12601 ttccagcatt tccaccagct ccaccttttt cacctttttt gccacttgta cctttttcaa
    12661 aatacccttt aaacatctgt ccgtggaaat aacttgtata tcctgcatca gctccattac
    12721 ttccagaagc tcctgtttca ccatttttcc catctattcc tttagaacca tttcctccat
    12781 ttgcaatcag tgttagacga taatttctaa tttcttcttt aatatataag taaatattac
    12841 ctgcatctct accatcttca ccatttttcc catacataga attatctaca agttctccgc
    12901 tctttccact tacattaatt tttacattac tacctacatc aagattgtta gaagcaagaa
    12961 taatattttt ctctgatgca aagtccattc caaggttatc atccatataa atagtatctt
    13021 tagcaaagaa cataaaagta gaaacattgt catctacttt tctatttttg aaatatttca
    13081 atacactgct aattactaag tttggaatta atgctcttat ttcaatacct ttcttatcat
    13141 taatattaat tgtttttact ataaatggat ttaaaatgta tttaccaata aattctctaa
    13201 ttatctcatt accttcttct ccatatgcac ttaatcttgt tatactgtct tctaactctt
    13261 cattttgtgt aactgtaaca tttgtcaaaa ttgaattaaa atcacttaca ctatatacat
    13321 aatccttttt atttgcaact tccattaatt tttctctatt tggtaatgta aatacctgtt
    13381 tttgcacttc ataagaaact aaaacttttt ctaaattctt tcttattata atgaagtttt
    13441 gttccattat cttcttactt gccttttcta taataaattc catatgtaca gaaggtaata
    13501 aaatttcccc agttccatgt tctgtatatt tacttaaagc atcaattata tctaagtttc
    13561 cttttatctt attttgtaat ttcatgcaac tatcttgtaa gaataatcca ctttgtttgg
    13621 ctaatacgtt gataaaactc tctatagtaa gacttgttgt actttccaac cagtttttaa
    13681 atacttttac aacctcatca tattttgcta tttcttgaat cattgttata cttccaataa
    13741 tattgataaa atcttctgta tacgccttaa cataatcttc taggctagat acgatattgt
    13801 tttgaatttc ttttatcaac gtatctaaaa ctaatcttgc atctgcacta attggatatt
    13861 ggaatgctga tgtcttatct ttaacttcat ctaattctag tacttgcttt aataagcctt
    13921 ccctctcatc tgatgcaagt ggcttacaaa gatgaatatt ttttccatta tcttttagca
    13981 attcttcaaa taaccaagct ttttcctcta tattactgtc taaattttcg cataattgtt
    14041 catattttct ttttataaca ttatatattt gtttttctgt tcttcctaag aaactttttg
    14101 aaatgaaaaa agttatacta cccttaaaat tttctttatg aagacacata tttaactgtt
    14161 ggaacaggtc tagtactttt gtaagtctag aatctaatat atctgcttct ggtatcaata
    14221 caattactgc acatatagat gcagaatttt taaatacttc acctaatgaa actgctgcac
    14281 taatatcatg ggctactccc ctattgtcgc taaaacctgc agtatcacat aattttgcat
    14341 ccattccttc tattggtttt ctcatattta caatgttcgg gtataaagtt tgagaaacag
    14401 ctcctgaacc aatctctgcc acagggtcta agcaatcaat atatgatata ccagtatctt
    14461 catcttctac ttccatcatt acacatcctg caagatagtt aattgcagta cttttgccac
    14521 atcctgtccc accaagaatt agaattaaat tttgattttc agccttattt attgctactt
    14581 ttccttcatt taataagagt gttatttctt ctaaaagttg attgttattt tctggcattt
    14641 attttccccc ttggttatat attttattta aataaagttt aatcccttaa gtagattgca
    14701 aagtttttag actatattta aatttgtttt ttatttttta tttttaataa atttgcattt
    14761 tcactaaatt tgcattttca ctaaataatt attcttcttt gtttattaaa ataactgtgt
    14821 tgtataatat aatctttatc aacacagtta ttttatttaa tgcgcttatt ttattgtatt
    14881 ttatgtaata cagttgtttt ttatattata tgtccattat ttttctatgg ttattacata
    14941 tagattttta attatatttg taccattaat ttaaacattc ctatactact gcttaagttt
    15001 tacacctata ccagcttgaa tattttattt ttatttgctt aaatagttca actatactgg
    15061 tttaaatagg ttatctttat ttgcttaaat agttcaacta tactggttaa ataggttatc
    15121 tttatttgct taaatagttc aactatactg atttaaatag gttatcttta tttgcttaaa
    15181 taagttcaac tatactgatt taaataggtt tcctttagtt taaataattc acctatactg
    15241 gtttaaatag gtttccttta gtttaaataa ttcacctata cctacttgaa tagttcactt
    15301 ttatttgctt aaattctata ccaatactgg cttaaacatt atactaccat gattgtattg
    15361 tctatcttca actacaataa aaacaggttc taaatcttta gtagatgtta tttttaattc
    15421 taaaaaaata ttatcagttg gtaatatttt tacatcataa ttatttaaat tattattcgt
    15481 gatactatta aatacatcac cttgacgttc aaaaaaaaca ttatcaacta ttacaaaata
    15541 aacatcttct tttctactat tttcaacctc tatagtattt tgtttagtta tatttagtct
    15601 catcttccac ctcctattaa tattacttaa ttatattaat acttgtaatt gttgcattat
    15661 gttcatcaca aggaagaggt ttaaaaacaa ctatctctgc taaataattc tgtggaggat
    15721 ttgtaactga agtagcattg caaataaatt taaaatttgt tcctgctaca acttgagtag
    15781 aaacaataag tggttcataa ccaacccctt ctaacattcc tacagcttca ttaaatactt
    15841 ttaaatcttc ttcttcgaca gctctgaatt ttgaccatcc accaacaaaa agactcatat
    15901 aaaaacctcc tttatatata tatttattta tatatagtag taccaatttt ctcaatattc
    15961 aatattttct gtgtaactgg tcaatgaaaa tgcataactg gataaaaata cttataaaat
    16021 aacttaactt tacatataaa tagcttactt tacatgtaat aagattattc tacatgtaat
    16081 aagattattc tacaatatga aatgcatctt gttttgaata tattctaaat acaataatgg
    16141 atattttcaa ttattataat tagaaaatat ccattactta acttgtttta tttaaaatca
    16201 catagagact ttattatttt ttttattgca tgaatagcta tttcaggtat cataaggtga
    16261 tataccccct ccttgaaacc acaaaaaaca tgacaaactt tacgaatctt gtcttaaaaa
    16321 actacaggta cattccttat ttgtggattg gagtagccat aacaagcaaa ttttacaatt
    16381 tttatgatga caaaaatcaa taaaatcgct tagtatataa cacgaaatac tacttgttac
    16441 tattaagaat agttagatag gaagtatgca ttatacaaat aaaggaatca ataaagaaag
    16501 ttaaaacatc cctacaaatt aaaaatctgt tacatttttc tatatttaaa cgttatttac
    16561 ttacaattgg agaaataaca ggttttcctt ctcggtcaag taaaggcgta aatccggctg
    16621 cattgccata atagtggaat acatagttca cacctgtttc tttatccacc caaatctccg
    16681 ttgcatttat ttttccttgt gaatatactt tttcaaatct atcttttccc attttataat
    16741 cccccatatt tttgattggt ctataccttc ttcacttaag tagaaaaaat acataactta
    16801 tttttttaat tctaatatga gcacgtaaaa atgtcaaaca gcttatttaa tttcataact
    16861 tcatgttact gttcgtaacc ttgtttgttt acttacacta cattacagca ccattttacc
    16921 ctttacatga ttgtattgca cacagcaaaa atgcttactt tattggattt ttcaactaaa
    16981 aaaaggattt tttctccaca gaacatttac tattttttat aatatatcta attttactct
    17041 catatcaaca tttaatgaag atataacctt tttcaaatat tgtaaagact ccttaaaaat
    17101 attagaaaaa gattctgcaa atctctcaac atctcctttt tttaaatacc taagtatctc
    17161 aaaacataca tcaaaacgtg ttttcaaaat ttcattatat tcttcatgaa aaaatctctc
    17221 taaatagatt ccaaatatca tatattgttt taaatgcaaa aatgtttcct gtaatacata
    17281 aatttgtgta taatctacta tcttacaaag taaaacaaaa tctggataaa ccttacactc
    17341 ttcacaatta ttttgtaagt accttaaatg agtttctatt ttatctacag ctatcaaatc
    17401 cattttattt cctagtaaaa gtactacatc ctcaagagta atcgtaacaa actccatagc
    17461 atccaaaagc aaactcaacc ttttagctcc ttcttctgta tttatataat catcatcata
    17521 ttcagaaaaa agtgttacat atgttccctt gccattaatt gttttagcaa ttcccaattc
    17581 atttagctca ttataggcac ttctaactgt tggaagtgat acattatatc tattaattat
    17641 cgaagtaatt gatggtagac aatctcctat tttaatattg ccttcaaaaa tttgacaaat
    17701 tacatctgaa acaattaagt catataaata acatttttcc aacgttacag aattaaaact
    17761 acttgtatct ctactttgtg gagcacggtt ttctaccatt ttaagtatgg attctctaca
    17821 agctttatac atatatgtta atagtttttt aaattcaatt agattcccgt ccaatccata
    17881 taagtaagtt tttcttacct gctctatgta tttattacga aaaaagtcta tacaacaatt
    17941 atccaagtta tcgaaattat aggccaattg tgaaataatg acatcatgct gaataatctc
    18001 acaaaaatgg tgaatcagga aattatttgt tttacttatt agtttattta tatattttag
    18061 cctttgttct ttaaagacta aataattaga actatttttt tccatacaat cgataatctc
    18121 gtttaattct tcaaagtctt tagatgtaaa tcttttaata ttgtacatag caatagaagg
    18181 aaatattaaa ctaaaaatct tatatatttc tgatatttgt aaaatcttct catataataa
    18241 gtcagattct attgattgtt tactcgtact ctggattaaa actggtctag aaccagcttt
    18301 tgtctctata aagccctttt ctgagagcat ttttattaca gtttttatag tattttttga
    18361 taccatatat tcttctgaca tgctttttat agaatcaagc ctatctccaa tagtaatttt
    18421 accagtataa atttgttgaa ttatatgctc aaaaataatt aaatattgtg gttttctctt
    18481 catatttttc ctcttcgata aattagattt attgtttttt gtaaggtata tttactttta
    18541 tttaataggg caaaatagtt gcttattttt tcctcataat ctataattaa tataaaccat
    18601 ttatatatat ggtctaagtt ttataatact gtaatgcagt aaaatagcat attgtagcta
    18661 tactacagca aattaacata ccattgtagt atattaattg gaataatttt aagtacttca
    18721 aaattccagc aaaacatata tgtgtttttg atattatgta tattcgttaa tagaatagct
    18781 atacttgttt tacataataa attatatgat acttgctata tgtaat
//
LOCUS       DAGSHT010000057        18095 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGSHT010000057 DAGSHT010000000
VERSION     DAGSHT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.059"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            355..900
                     /locus_tag="MJB72_001771"
     CDS             355..900
                     /locus_tag="MJB72_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBY8262885.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            913..1926
                     /locus_tag="MJB72_001772"
     CDS             913..1926
                     /locus_tag="MJB72_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBY8262886.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            2148..4121
                     /locus_tag="MJB72_001773"
     CDS             2148..4121
                     /locus_tag="MJB72_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY8262887.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            4190..4549
                     /locus_tag="MJB72_001774"
     CDS             4190..4549
                     /locus_tag="MJB72_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262888.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4584..4940
                     /locus_tag="MJB72_001775"
     CDS             4584..4940
                     /locus_tag="MJB72_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBY8262889.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4977..6269
                     /locus_tag="MJB72_001776"
     CDS             4977..6269
                     /locus_tag="MJB72_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBY8262890.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6705..7343
                     /locus_tag="MJB72_001777"
     CDS             6705..7343
                     /locus_tag="MJB72_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262891.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7350..8192
                     /locus_tag="MJB72_001778"
     CDS             7350..8192
                     /locus_tag="MJB72_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBY8262892.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            8253..10880
                     /locus_tag="MJB72_001779"
     CDS             8253..10880
                     /locus_tag="MJB72_001779"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBY8262893.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            11094..11339
                     /locus_tag="MJB72_001780"
     CDS             11094..11339
                     /locus_tag="MJB72_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY8262894.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11628..12926)
                     /locus_tag="MJB72_001781"
     CDS             complement(11628..12926)
                     /locus_tag="MJB72_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBY8262895.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(12960..16094)
                     /locus_tag="MJB72_001782"
     CDS             complement(12960..16094)
                     /locus_tag="MJB72_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBY8262896.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(16098..17321)
                     /locus_tag="MJB72_001783"
     CDS             complement(16098..17321)
                     /locus_tag="MJB72_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBY8262897.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            17573..18058
                     /locus_tag="MJB72_001784"
     CDS             17573..18058
                     /locus_tag="MJB72_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8262898.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
BASE COUNT         6602 a         2290 c         3057 g         6146 t
ORIGIN      
        1 aatttgatta agaaatagat gttcgtaaaa gagcatctat tttttatttt tataagattt
       61 agataatttt aataataaaa gaaataaaaa taagttctta aatttctaag aagaatttag
      121 aatatagtcg aaataagaat tgtatatttc tgaaaattct aattagtaca attttttcct
      181 ttataaaatt gaaaaaagat gtatttattt atcaaaaata ttatattaaa atgatattaa
      241 taaaaaaaca tttacaatat tagcatatag tacagaaaaa agatgtaatt tgtaaaattt
      301 atggtaagca gtaaaattaa aaatttaaag atattatagg gaggttataa ttttatggaa
      361 aacgttatta caggtctaag taaaggtgca gagtggttta tcggtttgtt tcagaaaggg
      421 ggagaacaat ttataggtct tgtttcaggt actcttccaa cgctaattgt attaatggta
      481 gctattaact ctttgattaa gataattggt gaggaaagag ttaataattg ggcttctaag
      541 ctaggaaaga actcttttac aagatatatt ctacttccat tagtatcagt atttttcttg
      601 ggaaatccta tgtgctatac atttggtaga tttttaaaag aagagcacaa agcaggattt
      661 tatgatgcag cagtttcatt tgtacatcca attacaggtc tttttccaca tgcaaatgca
      721 ggagaacttt ttgtatggtt gggaatatct gcaggtttga ctactttagg taaagaaact
      781 actacattgg cattgtggta ttttatagtt ggtctaattg taatttttat aagaggtata
      841 gtaacagaaa aaatgtatgc ttttctaaca agaaaaaata gagcaacaaa taaggcttag
      901 gaggtagata aaatggagga gaataaaata ttaaaaatag aaaagggaac ttcaggctgg
      961 ggaggtcctt tgtatataaa aaaagaaggg aatagaaata agatactatc aatgactgct
     1021 ggcggtatcc atgaagtaac attaaaaata aaagaactac tgggttgtga aattgtagat
     1081 ggatttaaaa ctggagtttc tgatgaagaa gtagcagtag ttattattga ttgtggtgga
     1141 actgctagat gtggagttta tccaaagaaa aaaataccta caataaatgt aaatccagtt
     1201 ggaaaaacag gtcctttagc aaaatttata acagaagaat attatgtatc agatgttaat
     1261 cctaactgta taagtgttgt agatggggaa gatatgccac aaaaatctca agaaaataaa
     1321 agcgaaaata agagttctat tagaaagcct gataattatg atgaggttaa gtcaaaagca
     1381 caaggtgaat atgctaaaaa gaacataata ctttctattg gtcaaggggc agggcaagta
     1441 gtatcaaaat tttatgatgc aggtcgagat actatccaaa tggtaatgaa taacgtaata
     1501 ccatttatgg catttgtaag tatgcttatg ggaattattt tagcatctgg actgggagac
     1561 tggattgcaa gagttatatc tccacttgca ggtaatattg gtggattatt gattatatca
     1621 gtaatttgca cacttccatt cttatcacca atactaggac ctggtgcagt tatagctcaa
     1681 gtagtaggga ctcttgtagg tacacaaatt ggtcttggtg caataccagc atatttagca
     1741 cttccagctt tatttgcaat aaatgggcaa gctggatgtg attttgtacc agtaggatta
     1801 agtttgggag aagcagaacc agaaactgta gagtatggtg ttccagcttt attttactct
     1861 cgtttaataa ctggtccaat agctgttatt attgcatatg gagttgcagt atttgcattg
     1921 agataatatt taatgtcaat ataaggcttg gaattaatta ataaaattgt aaatataaat
     1981 ttaagctagt caagattttt tgttaatgaa agactagctt ttatactatt aattagctgt
     2041 aattatcgag ttagattaat actaaatttt cctttattaa agtgaaattt tatgtattaa
     2101 tttggtttca aatttgtact agaatgaaat caaggaggag atacaaaatg agtgtaactg
     2161 agaaaattcc ttctattgac cgcaagatat ttaagatatt gattatgtgt agtaaaaaag
     2221 agtttgtgag tattaactca atagcaaatg aattgaatgt cacaactaga agtgtaagga
     2281 cgtatataaa acaattgaat aaagatttag gtaatgatat agcagtaatc aaatatataa
     2341 agggtcaagg ctataaacta gaaataaaag atgagcagat actcaataag attattgata
     2401 tgaatagaaa aaatgtattt tcattaaatt caaaagaaga tagagtagag tttatactaa
     2461 attacttgat agaactagat ggatttatta cacttgatag tttagctgat gagatgtgtg
     2521 taggaagaac tactttagta aatgatttcc agtatgtaga aaaagttttg gcttcatata
     2581 atttaaactt gattaaaaaa cagaatacag gtatgaaatt aaatggaaat gaacttgata
     2641 ttagactgtt tatattaaat cagttgtata aaaactcaag gaaagatttc agtaattcaa
     2701 agtattttaa aggaatcaaa aaagaagaaa tcataaattt agaagaaaaa ctattgactt
     2761 tatttaaaaa aaataatttt tatgttacag atgaaatgtt aagagaagtt attaactaca
     2821 tagtagtttt agtctataga gtaaaagaag aaaagaaagt aaaagattat gatgtaaagt
     2881 ttgatttact taaatcatat gatgaatact ttattgctag ggaaataaaa catataatat
     2941 caggaatgtt tgaatgtact ttaaatgatg aagaaattat ttatttaact ataccgttag
     3001 ttagtggaaa tgcaccagca tcagaatgtg ctttaaatag tagtagaatt agtaaaaaca
     3061 ttgatgagtt gatggaaaat atatttaatc aaatatatgt cgatatgggt atttttataa
     3121 atgaggatga attaagagta ggtctaggat atcatcttag ttttacttta aacaggctat
     3181 tatttaatat aaagttaaaa aatgttttgt tagaagagat taagcaaaat tatatacttc
     3241 catttaaatt agcacaaata gcaggaaaag taatagagaa aaagtataac ttagaagttt
     3301 cagaagatga gattggatat atagcaatcc atttcagtgg atatttagaa agaaatagca
     3361 gtagattcta ttcaataaaa aaaatagcaa ttatatgtag tacaggtcta ggaacagcaa
     3421 aactattgaa aataagagta gaaaaactga taggaaataa tcctaaaatt gatactcttt
     3481 caagttttaa tttgagaaat attaacttag atgaatacga tatagttttt acaactattg
     3541 atttagacac atcaaatata aatacaatag tattaaaaat aaatacaata tttgatgaaa
     3601 acaaactaag agaacaatta aaaacagtat tgtgcctaag agaagggaat attgacacta
     3661 ctaattctac aaatttacta ataaataatt tgttagatga agataaattt atgatattaa
     3721 atgaaaagac tatacttgcc tcattagaaa aaatgatgga ccatcttatg gatttgggat
     3781 gtattgatga gaagtttaga aaaaatatag tcaatagaga agaaaaatct cccacagtat
     3841 ttgataaagg tttattattt cctcattcta taaatgaaaa atcagataag tttttaatgg
     3901 cagttggaat tttagaagaa ccaatagaat atgcaaatag aagtataaaa ataattctta
     3961 tgacaatgtt tccatgtgag aataagatag actcagactt attagtaaaa atttatgaag
     4021 aagttttaaa gataggccaa gatgtaaaat taacgaataa gataagtaaa tgtagaactt
     4081 ttttagaatt taaaaaggtg ttacttaaaa accttatata actattgtac agcatttata
     4141 gttgttatac agatttaaaa ataatgtgat ttttggaagg tgtataaata tgaaattatt
     4201 agtaatttta tttgttgctt atattttaaa tactatattt ataatgaagc agaataaatt
     4261 gtattttaaa actttaaata gagctaaaaa aaagggtgat atagtgtcta caggcaaaaa
     4321 gaaaagctat ttttctaaag gaagtatagc cgttatatct tcagattcag atgggtttat
     4381 aaagtttgga gaaattttaa aagggcgtac agtaatggct aaatttaaag agattgaaga
     4441 aatagaagga ttagatatat atagtgcaga gcaaaaattt aaagatgaag agagtattgt
     4501 acaagctata aaatttataa aagaaaaaat aaatgtttct tttaattaac tcaatataaa
     4561 tataaattat caggaggaat gtcatgaaat atgaagttaa gataagtggg ataggcagtt
     4621 tagttaaaga gttaatggat gaaagcaatt gtcttattat atatgatgaa accattaatg
     4681 atgacgactt aaaggatata tctgtaattc attcaatagc aacattaaaa agtgatgttg
     4741 aaataggaga tacacttact atagggaata gagattattg tatagtttca gttggtgata
     4801 ttgctcagaa gactcttaga gaaattgggc attgtacaat caaatttgat ggaaaatgtg
     4861 aagtaaattt accaggagaa atacatgtgg aaataggaaa tccagatata actattggag
     4921 atttaataac tatttcttaa tttgaaaata aaattattat aacaggagga attggtatgt
     4981 tagatttaaa cagaaaactt gcaaaattgg aagaggaagg taaaaaaata aaagtagctc
     5041 ttataggagc tgggcatatg ggaaatggaa tggtaagcca aatggcaaat atgaaaggta
     5101 ttgaagcttc tattgttgta gatatagatt tggagttggc tcataaagct tttactgatg
     5161 caggaataga tgaagaaatt atagataatg taacaaatga aaaggatgca gaaataaaat
     5221 tacaagaagg aaaagtattg acatgtaatg actttcttgt ggcttgtaaa actaagtcaa
     5281 tagatgtagt tatagatgca actgggggta ttgcaattgg tgcagagata gcattaaact
     5341 ctattttaaa taaaaaacat atagttatgt taaatgctga aactgattgt gttgttggac
     5401 caatacttaa aaaattagca gatgatgctg gagtaatatt tacaggttct gcaggagatg
     5461 agccaggtgc agtaatggaa ttatttgatt ttgcagatgc aatggggttt gaagttagag
     5521 tgataggtaa aggtaaaaat aataaattag atttagattg taatcctgat acagttagag
     5581 aagaagcaga aagaaaagga gcttcacctc atatgatagc ttcttttaaa gaaggaacta
     5641 aaacaatggt agaaatggct ttaatgtgta atgcaactgg atttgtgcct gatgtaagag
     5701 gtggtcatgg tattgaagct acagttaatg aagtacctaa gaaatatgca ttaaaaagcg
     5761 aaggtggagt attagataat tatggtgtag ttgattttgt aaatggtata gcacctggag
     5821 ttttcgtagt agttgcccat aaattaaaag cagttaatga tgaactaaaa tatttaagta
     5881 tgggtgatgg acctaattac atattatata gaccatatca tttatgtagt ttagagactc
     5941 ctttatcagc agctatggca gttttagacc ataaggctac aatagtacct aaagctggac
     6001 tagtagcaga agttatgact atagctaaga aagatttgaa aaaaggtgag tttatggatg
     6061 gctatggagc atatacatgc tatggtacta tagaaaaata cgatgtagca aaggctatga
     6121 atgctgtacc tattggtttg ataagtaaaa agactaaagt tgtaaaagat attaaaaaag
     6181 gtgaagttat aacttacgat atggtagaga tagaaaaaga tactacttta tatcatttaa
     6241 gacaattgca agaaaaaata tttgggtaag atttgatggt aatataaata gatattagta
     6301 aaaagggttg atggttattt ttaaatcaac ccttttaaat ttttatatat tcttgttagg
     6361 atactttctt taaaagtata ttataggtta tttgtagtcg taaaaattat tttgaattag
     6421 ttctgcaatt ttatattcac attgaaataa tattattaaa aattttaata taaaatgaat
     6481 taaaatgacc aattatgtat tataatatta actgtaatac taatatagtt ctattaatta
     6541 aagaaatatc ccccattaat atataataaa tatctttaag cgagagaact tagtaaaaaa
     6601 tatatcgtat tcaataaaaa atctgtttac agaatggtat atatagttat ataatatact
     6661 atataagatg cgaaaagata aaaagtatag cacaatgaaa gaaggtgatt attattcagc
     6721 ttaatgaaag acaactaaaa attatagata tagttaagga aaatgaacca ataacgagtg
     6781 aaagtattgc ctccagcttg aatgtgactc gtgctacact tagatctgat ttagctatat
     6841 taacaatgac aggaatatta gacgcaagac caaaagttgg atatttttat tcaggagtta
     6901 gtgaaataaa tttaattggt aaaagtgtaa aagaaaaaac agtagaggat ataatgagca
     6961 tgcctgtact agctaagaag gatgaaagta tatatgatgt aatagtcact atgtttttat
     7021 ctgatgtagg gagtatagtt attatagatg agaatgaaga gttatgtgga gttgtttcca
     7081 gaaaagactt gctaaaagca actataggag gttctgatat aaataaaatg ccaataggta
     7141 tgataatgac aagaactcca aatgtagtca ctttaactaa aggagcaagt gttttattag
     7201 cttcaagaaa aataatagaa catgaagtag attcgattcc gattgttgaa tataagggag
     7261 aagacaaaaa tcatatgaga gttgttggga gaatatcaaa aaccaacata actaagctat
     7321 tcttagaaat agttgacaat taaggaggta tgctggtgaa aaacctaatt atatatgcag
     7381 tatcagattc agtaggggag actgcacaac aagttgcaaa ggcatgtatg tctcagtttt
     7441 atgtgaatga aacttatgaa ataaaaagat ttccatacat gattaataaa ggagtactac
     7501 ttgaaacttt ggagaatgca aaagctgaaa acgctttaat tgtatataca ttagttgatg
     7561 aagaattatg cagtattgtt gaaagatatt gcgaaagaga aggtcttagt tgtatagatt
     7621 taatgacaga tatactaaga gaaataagca agagaacagg aagaaaacct aaaagagaag
     7681 ctggtataat tagaaaatta gatgaatctt actttaagag agttgaagct atagaatttg
     7741 cagtgaaata tgatgatgga aaagacccta gaggtgttct tcaagcagac ataattttag
     7801 tgggaatatc aagaacatct aaaactcctt tgagtatgta tttagctaat aaaaatataa
     7861 aagtagctaa tgttccatta gttccagaaa taccaattcc taaagaagtg tttgagatag
     7921 atactaaaaa gataataggt cttactaatt cacctgaaaa gctgaatgag ataagaaccc
     7981 aaagattaaa agctttagga ttgtccagta aagcaaacta tgctaattta gaaagaatac
     8041 ttcaggagtt agattattca gaagagataa tgaaaagaat tggatgccca gtaataaacg
     8101 tttcaaataa ggcaatagaa gaaacagcag gtataatttt agacataatg aaagaaaatg
     8161 gtctaaagat atacaaagaa atagaaattt aataatgtct attaataaaa atgatataaa
     8221 ttaaaataaa aagattaatg ggggatttaa caatggaaac taagtatgtt tatagttttg
     8281 gtgaaggaag caaagacatg aaatctttac taggaggtaa aggtgccaat ttagcagaaa
     8341 tgactaagat aggcttacct gtccctcctg gattcactat aacaacagaa gcatgtaatg
     8401 attattatgt taacaatgaa agtataagag cagagataat aaaagaaata gaagagcatt
     8461 tagctacttt agaaaaagac ttaaataaaa cattaggatg caataagaat ccattattag
     8521 tatcagttcg ttctggtgca gtattctcta tgccaggaat gatggataca attcttaacc
     8581 taggtcttaa tgacaatagt gttgttggct tagcagaggc tacacaaaat gaaagatttg
     8641 catatgatag ttacagaaga ttcatacaaa tgttttctga tgttgctatg gaagttccaa
     8701 aatataaatt tgaaaatgta ttagacagag taaaagaagc aaagggatac acagttgata
     8761 cagaacttac aactgatgat ttaaaagaaa tagtaaaaga atttaaagca atttataaaa
     8821 aagaaataaa aaatgatttc ccacaagacc caaaagagca attaatgctt gcaatagagg
     8881 ctgtatttag atcatggaat aatcctcgtg caatcgtata ccgtaaattg aatgatatag
     8941 ctcataactt aggaacagca gtaaatattc aatcaatggt atttggtaat atgggagaaa
     9001 ctagtggtac aggtgttgca tttactagaa atccagctac aggagaaaat aaattatttg
     9061 gtgaattttt aatgaatgca caaggtgaag acgttgttgc aggtataaga actcctcaaa
     9121 atatatcaac tcttgcagat gtaatgccag ctgtattcga tgaatttgta aaaattactc
     9181 atatacttga aggtcattat aaagatatgc aagatataga gtttactata gaaaatgaaa
     9241 gattatatat actacaaact agaaatggta agagaacagc agcagcagca ataaatgtag
     9301 cggttgattt agttgaagct ggaataattg atgaaaaaga agctatcatg agaattgagc
     9361 ctaatcaatt agaccaatta ttacatccaa aatttgaaga taaggcatta aaagaagcta
     9421 aggttatagc aaaaggttta ccagcatcac caggtgcagc aagtggtaaa gtttatttca
     9481 atgctgatga tgtagtaaaa gcaaatgaaa aaggagaaaa agtagtttta gttagactag
     9541 agacatctcc agaagatata gaaggaatgg ttaaagcaga aggaatcctt acagctagag
     9601 gtggaatgac atcacatgca gcagttgttg ctagaggtat gggtaaatgt tgtgttgctg
     9661 gatgtggaga aataaaagtt gatgagttta ataaagaaat aagagcactt gatgatgtag
     9721 ttataaaaga aggagaatac atttctatag atggttctac aggaaatgta tacctaggag
     9781 atgttaagaa aacagaagta tctttaactg gaaattttga aaaattaatg aactgggtag
     9841 acaaacacaa atgtatgatg gttagaacta atgcagataa tcctagagat gctcgtgcag
     9901 ctatagagtt tggtgcagaa ggtataggtc tatgtagaac tgagcatatg ttctttgatg
     9961 aagctagatt gccagcagtt agagaaatga tattatctaa tacagtagaa caaagagaaa
    10021 aagctcttga aaaaatatta ccaatgcaaa gagaagattt tgtagagtta tttaaagtta
    10081 tggatggaaa accagttaat ataagattac tagacccacc actacatgaa tttttgcctc
    10141 atgatgatga gactatagaa gaactttcaa aatctatggg aataaaagta agtgatatta
    10201 agaagagaat agttgattta gatgagttta acccaatgct tggacacaga ggttgtagac
    10261 ttgcaataac ttatcctgaa atatgtgtaa tgcaatctaa agctataata caaggtgcta
    10321 tagaagcaat caaagcaggt gttaaagttt ctccagagat aatggttcca ttagttggag
    10381 aagtaaatga gttaaaaata ataagaaaaa tgatagtaga aactgttgac gctataataa
    10441 aagaagaagg tatagaagtt ccttatactg taggtactat gatagaaata cctagagctt
    10501 gtttaactgc tgatgaaata gcacaagaag cagacttctt cagttttggt actaatgact
    10561 taactcaaat ggcatttgga tattcaagag atgatgctgg taaattctta ggtcaatatg
    10621 tagatgaaga aatattagaa aaagacccat tccaagtact agaccaaaat ggtgttggta
    10681 agttagttaa gatgggagct aaattaggta gagaagttaa accagaatta aaactaggaa
    10741 tatgtggaga gcatggagga gaaccatctt cagtagagtt ctgttatagt gtaggactta
    10801 actatgtatc ttgttcacca tttagagtgc caattgctag acttgcagca gctcaagcat
    10861 ctataaaaaa tcctagataa taatagactg caaaatatgt aactaaataa aaataaaatc
    10921 ccctattgtt caatgtatca aattaagtat ttgtaaattg aatatagggg atttttatta
    10981 taaaaattta acttatatag ttgtaagtga aacgtattta attatattgc atatattatt
    11041 gtagagagaa atttcaacta tcaattaaat tgagtttact ggaggggata aagttgacaa
    11101 agtgtcctaa atgtggaaag gaagtatcat cacgtccagg tagtatatgc ccacgatgtg
    11161 gatttaaagt agcagatgag agtctaaaaa taagatgtcc agaacctagt tgtagggctt
    11221 tggtatctag gaaattggat tattgtccta aatgtggatg caaacttaga ggatataata
    11281 tttctgaatt tgttaacatg gtgagatgta aaatagatga gacatttgat aaaaaataat
    11341 taaatttaga gttaagcttt tgagggtgtc tcaaaatgaa ctttttagtt cataaggcac
    11401 tctttttgta tggaattaaa tatattttta taatttaaac aataaaaaag aggcttatct
    11461 ctattttgag atagcctcta taaaattata cagttttata agcaaatata tttttactaa
    11521 tctatttcta ttatttgcct tctgaatctt ccatgactaa agccacgagt gtgcataact
    11581 attttttaat caaacgtatg ttaaagtaat ctttctattt accagaatta ttttccagta
    11641 ttaagtatcc aaggtttttc tataccattt tttagtgagt tataattcac aataagagtg
    11701 tcatagctat ttttagcatc ttcattactt accacttgtt ttttataatc agttagagta
    11761 attaatccta aatcatatct taatttagta ttttttaatt gtttttctga taaatcaagt
    11821 tgttttttag taatctgaat tgtatcttct aaagcaagta attgagcata gctatcaatt
    11881 aacccttttt taagagcttt tttactgtct tctaatgtta ctagaccatt ttcggtatta
    11941 aatttttgtt gaagataatt tgcataagta gtaaggccag cagcataagt ctgtctttca
    12001 agattgtatg cttcaacttc tttttcatat ctcttgtagt ctttttcata cttatccgta
    12061 agaaccttat attcatcatc agttaggtta ccttgctctg gttttgtagg agcaactggt
    12121 cttgtaagat taggtctatc tggtaaatct gctttgttct catctttatc attaaagctt
    12181 tcttctgtac gttctaaaat caattggtcg tattttaaat attcattaac cttaccttcc
    12241 atatatgaat caactgagcc acctactcta aaagattcaa atctatattc tttatccaat
    12301 tgataattat ttaaatctat attagtcaac aacttaaagt agtcttgatt gttttttagt
    12361 tcttgaagtt tagctttttg agtattttgt agtgtttgaa gttcaagttc agctgattca
    12421 gtttctaaag atgttactaa ccctaaatct ttttttaatt tcatatcttt aatttcttta
    12481 gtttttattt caagattgtt ttttatcttg tctagctcat tttctcttga aactaaatcg
    12541 ttaaatttat tagtaatatc atttgctatt tggtcctcca taaagccttt agcctgttca
    12601 ctttgatttt taagaagtcc aatttggtcg tatggaaacg agttgtcact tccagtaaaa
    12661 ctatcaaact catcttgtac ttctaacttt tcttttaaca tttttatttc ttctgatttc
    12721 atttttaatt tttcactatt tttaagtgca gcactaacag cttctctagc agtcaaaatt
    12781 tccttttcag atgttctttt atttgagtca cttaaaatag aattatcaac tttgtttata
    12841 gaagaagtat tattatctgc gtaaactggg attaaattgg cagttattat accaacacca
    12901 cagccaattg caactatttt gctcattttt ttattcaata caatcaccct ccattctatt
    12961 taaactttag ctttcaattt tggtttcttt attattttac gttttatact tcctatttta
    13021 tctgttatct taccaaatat tttaggcatt ttgtctgaaa acttattttc ccatttatca
    13081 aatattaagt atatagtagg tataagcacc aaagttataa gtgtagatac agttagacca
    13141 cccattataa ctatggctag tggttgcata gtttctccac cttcaccaag agatagtgct
    13201 gttggcaaca tacctaaaat tgttgttgct gtagtcatta agactggtct taatcttgct
    13261 gcacaaccaa ttgaaaccaa accaattaaa tcattgttac gatttgtttt tctaagttgc
    13321 tctatgtagt caataagaac tataccatta ttaacaacaa taccgataag taagattata
    13381 cctagcatac cgacagtact taaactttga ccactaacta gaagcgaaag cacaactcca
    13441 acgaatgcaa atggaacaca gaacatgatt ataaatggtt tactaaatga ttcaaactga
    13501 gctaccatta ccatataaac taatgctata gctatgacca ttgaaaatcc aagacttgcc
    13561 atagattcat tcatcatttc tgtttcacca ccaacaccta tagagtaatc tttaggaatt
    13621 accattttat tcgttttttc tagtgctagt tttgtagcag taccagcatc taaattgtca
    13681 agtgtagcag ttatatttac agaaaaatct ccatcagtcc tagatataga ttgtagacca
    13741 tctgccattt ttatatctga aaaagcacca agaggaactt cttttccagt tgcagagttt
    13801 acttttagtt gctttatatc ttctaagcta tcaatagtat ctgctttaaa ttttaggttt
    13861 acatccactt tgtaatcatc aatagtagca gtggttacgg agtcaccatt tattgctgta
    13921 cgtaacatgc ctgcaatttc agcggtattt acaccatatt cttgagcctt atgtttatct
    13981 atagtaaatt gagcttcttg tgttgtatct gtaagagaag tttcaacatt tctaaatcct
    14041 tttatagagc taaattcttt ttcagcttct tttcccagta tttttaatac atctaaatca
    14101 ggacctttca tttctaatgt gaagtctgct cctccagctc cacccataac actactgttt
    14161 tgacttacat ttattttaca atctggtaca gtagccactc tatcaattat ctctttttct
    14221 atttcatcag tagttctttt tctatttttt tgagattcta attcaattgc tatacttgct
    14281 ttactattat ttgaatctga tgtcaaagtt gttattaaag ttttaatttc tggaatatct
    14341 gagacttttt tctcagtcat agatacatag taatcattgg attctaggct aagacctttt
    14401 ggaagttcta tagaaataga taattcaccc ttgtctgctg caggcataaa gtccatacct
    14461 ataaatccca ttccaaataa ggaagctaca aaaataagta aacttgatat tacaactaag
    14521 cgttgatgtc tcaaacatac acttaataat tttttatatg cttcacttac cttatcaaat
    14581 ataggactat ctttttcttg catcttttga ctattttttc ctctgcttaa ttttgcaaat
    14641 acactaggaa cgagtgtaat tgcacctata aaggagaatg ctagtgaagc tattagtgtt
    14701 ttacctaatg ccccaaaagt tattttagct ataccttctg taaacatgat aggtaagaat
    14761 attgctattg tggtcaatgt tgatgcaagt attgcatttg ttacagttct tgttccctca
    14821 atagcacaat catcaagact caatctgcca cttttcctat atttaaatat attttcaatt
    14881 acaactgtag cattatcaac aaccatacca actgaaatta ccaatgaact tgcagtaata
    14941 agatttaaac tttcaccagt aaagtagaga attgctatag cacctattat agaaattggt
    15001 attgcaactg caacaactag tgaagctctt acacttttta ggaaagcaaa tattacaata
    15061 aatgcgataa cagcacttat aagcaagttg ctcattacac tgtttattga ttcacttatg
    15121 tatgaacttg tatcattagc aattttaatt ttaaattgag gattttcctt attcaattca
    15181 tctacgtatt tgtatgcact ctgcataact tttacagtgt tggcatcttg ttgtttagaa
    15241 atatctatta ccaaactgtc ttttccattg tatctataaa tactatcttt gtctgcatat
    15301 ccataatcta cactacaaag attatctaat ctaactgtat ttcctccttt tacaggaatt
    15361 tgaatctgtt ttatatcatc tagggattct aatttatcaa tcgctcttaa aactatttta
    15421 tcctcacctt gagtaattga accataaggg aatgttttat tactagagga taagacacct
    15481 tttatagtgg ataaactaac cccataattt gaaagaacag ctgggtcagc tattacatta
    15541 acctgtgctt tttttccacc tttaaggtct gctgaagtaa caccatctat ggattctatt
    15601 ttaggctgaa ttacatcttc agcgtatttt cttaaggctt gtgaactatt ttcatttcct
    15661 ccagatatga caagttgacc tatggcctgt gcattcatat ccagctttaa aactgttggt
    15721 ttagctgcat catctgggag gctggtctgt aatgcatcaa ctttagacct aacatcattc
    15781 attatttcat ccaaattagt tccaaattca aactgagcaa ctaccataga aacactttct
    15841 tgtgaacttg atatagtaga ttttacattt gaaacggcag ataaactttc atcaactttt
    15901 tgactaactt gttcatcaac gtcttgagga ccagcacctg tccaagtagt catgaccatt
    15961 acaactggta tttctatatc aggcattaga tttattgaca tttttttata tccaatacca
    16021 ccaaaaacta ttaatactaa aaagaccatt atgattgtga gaggacgttt aacggaagtc
    16081 tgtgttaaat tcatagtcta cttctccttt tctacaggaa atatcttagt tccgtcagaa
    16141 ataagactta tccctccaat aatcactgta tctttatcac ttacaccact tttaatttct
    16201 attttatcct ctgttacaat tccagtttga accaagtgtt tctttgctat attgtctttt
    16261 gttgctatgt atacatattt tttaccattt tcttcaaata ttgctttttt aggtactgta
    16321 acagcaccat tttctttttc aacacttaag tttacttgag cagacatacc tgccttaaat
    16381 ttgttgtttg tattatttac aagtacttta acaggatata aagatgtctg agggtcaact
    16441 actgaaggta cataatctat ttttcctaaa atcctttggt cttcaattat aacatctaca
    16501 tttccatctt tcttaaattt acctatatct gattctgcta catttaaatc aacttctagt
    16561 atactaggat tagaaataat aaaagctggt tgtgattgtg ttatcatttc ctttgaatca
    16621 aagtttttag ctgttattgt accatctact ggagatgtta atgttaattt gtctaaatta
    16681 ctcattgcta aattatatga tacttgagct tggtcaagct gttttttagc aagtgcttta
    16741 gcttgaggta tactttttga agttgctgta tcgtatgctc tttgagcaga atctaactgt
    16801 tgttttgttt gctctaaaga aaattctatt tttttaaatt ggtctaaact aatgacttcc
    16861 tgttcataaa gaatcttatt attgtcataa tttctttgag actcctcata ttgaatcttt
    16921 gcattgtcaa gagctgtttt tgcctgattt acttgtgcct ctaatgaacc accagtggca
    16981 cttgaataac ttgcttttgc taaatctagt gaggctttac tttgctttac actattttct
    17041 agctctggac tgcttatact caataattca tctccttttc ttacattttg cccaagtttt
    17101 acatacactt cttgaactgt gccaacagtc tgtgcagtaa cagctgtttc atctttaacc
    17161 tttgtagttc ctgagaaaga atttgtgttt tcaatttctc cacctattgc tttttgaaca
    17221 gaaactgcta ttggtttctc aggttcagat tcttctgttt ttccacatcc aactaaaaac
    17281 agtgaactac acattaagaa aactattaaa tatcttttca tatctcctcc tattatatat
    17341 tgcacactag cttgttgtag aatttaaagt gtcttataat ttaagtttag ttttagacta
    17401 attaaaaaac ttccctttat tggcaattta taattctcta atttgattat aaatttataa
    17461 ttattatgag tcaataactt tttcttgttt taaatttatt aatagtttat aatttatact
    17521 atattaaaca acaatgtttt attatattat ctattgaagg gatgttacaa atatggataa
    17581 caacaatata cttaaaatag cggactcttt tttagatttt ttatttgtgc ttgaaaataa
    17641 tatatttaaa gaaagcgatt tgttaaaaaa gtttcaaaat aactctgata taatgaaagc
    17701 ttattttgaa gaatgtccaa tggctccatc acatgctaag gtaattcttt atcttatgac
    17761 atcaaattca tcatcaattt ctcaaatagc ttctaattta ggaattttaa aatcaaatat
    17821 gactccgatt attgacagat tagtggaaca tggtttagtc aataaatttc ctgacccaaa
    17881 agatagaagg attcttaggg ttgaattaac agataaagca tttaaattat ttgatgcagt
    17941 aaaagctatt ctcaaagaat ctctagtaaa aaaattatct aatctttctg aagaggaact
    18001 aactttatta gatgaacata cattaaagtt atcagaaata gtaaagaaat taggttagag
    18061 tgtgattgta aaagtcacta ttaaaaaagt taaaa
//
LOCUS       DAGSHT010000058        17735 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGSHT010000058 DAGSHT010000000
VERSION     DAGSHT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.060"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(80..643)
                     /locus_tag="MJB72_001785"
     CDS             complement(80..643)
                     /locus_tag="MJB72_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY8262899.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            796..1017
                     /locus_tag="MJB72_001786"
     CDS             796..1017
                     /locus_tag="MJB72_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262900.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            1020..1836
                     /locus_tag="MJB72_001787"
                     /pseudo
     CDS             1020..1836
                     /locus_tag="MJB72_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
     gene            complement(2015..2194)
                     /locus_tag="MJB72_001788"
     CDS             complement(2015..2194)
                     /locus_tag="MJB72_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBY8262901.1"
                     /translation="MSTSLPKYTLRINRVLLEKIKYIAESEGRSANKEIEQIIKKHIE
                     DYEQRKGEIKINIEE"
     gene            2271..2408
                     /locus_tag="MJB72_001789"
     CDS             2271..2408
                     /locus_tag="MJB72_001789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262902.1"
                     /translation="MKHEQTTLRIPEDLYKALIDLSSEIGMPIASIIIIACWLYISKI
                     N"
     gene            2419..2643
                     /locus_tag="MJB72_001790"
     CDS             2419..2643
                     /locus_tag="MJB72_001790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262903.1"
                     /translation="MQNECTALFIRELTDKLFSLAVFSSNSFRTLGILRVERTLSSLL
                     YEIDLSSLTFEFLIMSLIAYRISEDLRIPV"
     gene            2827..2997
                     /locus_tag="MJB72_001791"
     CDS             2827..2997
                     /locus_tag="MJB72_001791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262904.1"
                     /translation="MEKVRMTVRLVPKLNQDVQKIAKKCGKSKNSIIIDACWDFVEKI
                     KKENKFNIESEE"
     gene            3102..3314
                     /locus_tag="MJB72_001792"
                     /pseudo
     CDS             3102..3314
                     /locus_tag="MJB72_001792"
                     /inference="COORDINATES: protein motif:HMM:NF021206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            3396..3803
                     /locus_tag="MJB72_001793"
                     /pseudo
     CDS             3396..3803
                     /locus_tag="MJB72_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
     gene            3850..4059
                     /locus_tag="MJB72_001794"
     CDS             3850..4059
                     /locus_tag="MJB72_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262905.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(4069..4443)
                     /locus_tag="MJB72_001795"
     CDS             complement(4069..4443)
                     /locus_tag="MJB72_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262906.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            4519..4716
                     /locus_tag="MJB72_001796"
     CDS             4519..4716
                     /locus_tag="MJB72_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8262907.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADCLDITLDELVGR"
     gene            4747..5139
                     /locus_tag="MJB72_001797"
     CDS             4747..5139
                     /locus_tag="MJB72_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8262908.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            5120..5269
                     /locus_tag="MJB72_001798"
     CDS             5120..5269
                     /locus_tag="MJB72_001798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262909.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            5340..5777
                     /locus_tag="MJB72_001799"
     CDS             5340..5777
                     /locus_tag="MJB72_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262910.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKSGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            5789..6955
                     /locus_tag="MJB72_001800"
     CDS             5789..6955
                     /locus_tag="MJB72_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBY8262911.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYIDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            6970..7305
                     /locus_tag="MJB72_001801"
     CDS             6970..7305
                     /locus_tag="MJB72_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262912.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVTVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            7358..7930
                     /locus_tag="MJB72_001802"
     CDS             7358..7930
                     /locus_tag="MJB72_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBY8262913.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            7953..8525
                     /locus_tag="MJB72_001803"
     CDS             7953..8525
                     /locus_tag="MJB72_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBY8262914.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPIRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            8525..10483
                     /locus_tag="MJB72_001804"
     CDS             8525..10483
                     /locus_tag="MJB72_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBY8262915.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVSFEITSLTKESIPEILKQVDD
                     ENVVRTLELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKVPIESIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            10495..12912
                     /locus_tag="MJB72_001805"
     CDS             10495..12912
                     /locus_tag="MJB72_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262916.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNYYTRTI
                     IRKVLVAAVARIFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKINKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            13193..13519
                     /locus_tag="MJB72_001806"
     CDS             13193..13519
                     /locus_tag="MJB72_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBY8262917.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKPSKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            13468..14838
                     /locus_tag="MJB72_001807"
     CDS             13468..14838
                     /locus_tag="MJB72_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8262918.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IYNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            14862..14999
                     /locus_tag="MJB72_001808"
     CDS             14862..14999
                     /locus_tag="MJB72_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262919.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            14992..15471
                     /locus_tag="MJB72_001809"
     CDS             14992..15471
                     /locus_tag="MJB72_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262920.1"
                     /translation="MDKESIKEIVRELRKEEKENKKREVFHNTKLLLKHYNDLKSHIA
                     NSISDIEDVKSDYDDLLCLDIEDLYILSIKRSKAKTLIMVAHIDMALETLKVTQNKLQ
                     SLEKYKALELYFFKEKTYEDIAELLHCGVATVRRWVNEMVKELGIYLFGVDGLKIKL"
     gene            15610..15879
                     /locus_tag="MJB72_001810"
     CDS             15610..15879
                     /locus_tag="MJB72_001810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262921.1"
                     /translation="MDMINLEEIFCKAKLLDKKYISLKINITEYPSEEIIIIQNTNFE
                     SKLRYYKEIYDYKLNHKYVDGIKIIDCTYGNDFKEIELYINKNLD"
     gene            15946..16107
                     /locus_tag="MJB72_001811"
     CDS             15946..16107
                     /locus_tag="MJB72_001811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262922.1"
                     /translation="MEKKEIEELSKICEPILEYLNKKYTPHNSIVISTDGIKLLSIEI
                     GIPVKHQND"
     gene            complement(16135..16335)
                     /locus_tag="MJB72_001812"
     CDS             complement(16135..16335)
                     /locus_tag="MJB72_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HBY8262923.1"
                     /translation="MNMMPVSSRRIRSVGWENNTLYVQFHNGVTYAYLNVSETEYQDF
                     LNSPSLGSELSRLDKIHPYHKL"
     gene            16478..17410
                     /locus_tag="MJB72_001813"
     CDS             16478..17410
                     /locus_tag="MJB72_001813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262924.1"
                     /translation="MANRKVMYYCVELKNVSNGEKVSSEQMKVELNKIFDDKCISHEE
                     LKHLILKDKNDAFERITMDIISNEEDYLFGRVGKLKGNGDILLRNIDTNKANEIVEKN
                     KRPEIYTYFMLDYAHGILGYLNNQSLPKPDMLKRIFDLNNSCFEMNIKNVINPQTVRE
                     LMTPGSKLSKIEYSYKVPSIYVLQSLGLSRETVADLENTDYQTVTIQIASESRQKLTE
                     KSETIKKLIKHFSTNKNLENKVFKGKAPNDAMRNYKFDVDNYSLIIDVPRDKIEGGKT
                     KIYTLDEITEEVKIRMKEKYINNRKHILRLCGIE"
     gene            17441..>17735
                     /locus_tag="MJB72_001814"
     CDS             17441..>17735
                     /locus_tag="MJB72_001814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262925.1"
                     /translation="MKNKQETVKKKRFLFMLVLFSCVYYFLIVKGKINLSILTNTTDL
                     SNHFNFITVNSVFVGFLFSSLSLILGLSGTSSIIRLEKAGFMENIYRNISMGIF"
BASE COUNT         7379 a         1825 c         3275 g         5256 t
ORIGIN      
        1 aataaaaatg gtgtagcatt aaatcggttt atgacatctg atattataga tttcatataa
       61 tttatcacct acttttaatt tattttttat acttatttat taaaaattca atataatcat
      121 tgagttgttc ttgagcttcc tctggtaaat ctttgtgagg attaactcta tgtgcagcta
      181 cagtgtctat atcatttttt acaatagacc tacctaataa ataatcagta gtcacattaa
      241 atatttcagc aaggctagat aacatataag catcaggaac tgtctttcct tgttcataat
      301 aaccataagc gcttttggtt aagtttaatt tatttgcaac atcttcttga gtcatatttt
      361 tttcagctct aagttctttt agtctttttt gaaataatgt attattacaa gaagttttgc
      421 tagaaatatt gcttgcttta ttatcatacc tacctattaa ataatcaaca gtagtgccaa
      481 aatattcagc taaactagat aacatttctg gggtaggtgt tcttttacct tgttcataaa
      541 atccatatgc actagtagtt atgttaagat agttggcaat ttctttttgc attacacctt
      601 tttctttcct aagaaatttc aatctatctg ataaaatgct catataatca ctcctcttaa
      661 taaagttgcc tttattaaat aatacaacaa atagttgtaa aatgaaataa aaaaataaaa
      721 aaactattta caaacaacat atagttgtgt attatataag tataaacaac aaaatgttgg
      781 caaggaggat atagcttgaa caatctaaag gaacttagga cagaaaggaa tttaaagcaa
      841 aaagatgttg cattaaaagt aggtataact actagttatt atggaatgat tgaaagtgga
      901 acaagagtac caagtttagc aatagcaata aaattatcta aatgttttgg agtatcaata
      961 gaaaaaattt ttttaaatac aaatacaaca agatgttgtt taaaagagag gagttaaata
     1021 tgaaagattt aaaaatagta aaagttaata ataaactaac aactgatagt agggatatag
     1081 ctctaatggt tgaaaaagaa cacaagattt tactaagaga tataagaaac tatataaatc
     1141 agatggaaga agccaataaa aacatgagta cagatttgta cccatctgat tattttattg
     1201 aaaatactta tttagatgat tataaaagag agaagccatg ctatgcaata acaaagatag
     1261 gttgtgactt catagcaaat aaaatgacag gcataaaagg tacggcattt acaggaatat
     1321 atacaaaaaa atttgaagaa atggagcaag tattaaaaaa tgaacaaact aaactaccaa
     1381 ctacatataa agaagcgtta caacatctta tagagcaagt agaagtaaat gagcagttac
     1441 aattagaagg taaaatgaaa gaccaagtaa taaaagaatt aaaaccgaag gcggattata
     1501 cagatatgat acttaaaaac aaaggacttg tcacaataac tcaaatagca aaagactatg
     1561 gaatgagtgg aaaagaaatg aataaaatac ttcatgaaag agggattcaa tataaacaaa
     1621 gtggacaatg gcttttatat aaacaacatc aaggggaagg gatacactca ttcagaaaca
     1681 atagacataa ctagaagtga tggaatgact gatgtaaaaa tgacaactaa gtggactcaa
     1741 aagggaagat tgtttttata tgacttattg aaagtaaata acatattacc agatatagaa
     1801 aaagagtata gttatcaaac ttcaatgttg ggttagtact ttgaaaaata aatacagaat
     1861 attcaaaaag aggtgattta aaggttgagt aatagaaaga agtatacctt gagtattacg
     1921 aaagaattat acaataaatg taaagaaaaa gctaatcaaa gaggtatgtc agtgaatgat
     1981 tacatactat cagtgattag caagagtata taaattattc ttcaatatta attttaattt
     2041 ctccttttct ttgctcataa tcttcaatat gtttttttat tatttgttca atttccttat
     2101 tagcagaacg accttcgctt tcagctatat atttaatttt ttcaagcaga actctgttta
     2161 ttcttaaagt gtatttaggt agtgaagttg acataataat acctcctttt taaagtctaa
     2221 atgatgtcat aattatatca taaaaataat taaagaaaga gggagataaa atgaaacatg
     2281 agcaaacaac gcttcgtata ccagaagact tgtacaaagc gttgatagat ttaagtagtg
     2341 aaataggaat gcctattgca tctattataa taattgcatg ctggttgtat atatcaaaga
     2401 taaattaacc aatgagatat gcaaaatgaa tgcacagcat tatttattag agaattgact
     2461 gataaattat tttctttagc tgttttctct agtaattctt ttagaacact aggaatcctg
     2521 agagtagaac gaacgctatc atcactttta tatgagattg atttaagttc attaactttt
     2581 gaatttctaa taatgtcatt aatagcatat agaataagcg aagatttaag aatacctgtt
     2641 tgataagaaa ttttttctaa attttcattt aatggtgtat atattcttac agtaatagta
     2701 gtcattttga cacctcccta atgacattat acagaagatg tttagaaaag tcttgacagt
     2761 aaaacagtgt cataatataa tggatataga cagtgaaacg gtgtcataaa gagaggagga
     2821 ataagaatgg aaaaagtaag aatgacagta agacttgtac caaaattaaa tcaagatgtg
     2881 cagaaaatag ctaaaaaatg tggaaaaagt aagaattcta ttatcataga tgcttgttgg
     2941 gattttgttg agaaaattaa aaaagaaaat aaatttaata tagaaagcga ggaataagta
     3001 tgaataactt aacaattatc aagcaaaaca ataaatattt agttgagagt agagaggtag
     3061 cagaattaat ataaaaagac cataaccaat tattaagaag tataagagga tatataagtg
     3121 tattagagca gagtgcaaaa ttgcacactg atgatttttt tattgaaagc acttataaga
     3181 atgaaaataa tcagaaatat ccttgttatc tattgactaa aaaaggttgt gatatggttg
     3241 cgaataaaat gactggggaa aaaggcatta tatttactgc tatttatgtg gctaagtttg
     3301 aagaaatgga gcaagtatta aaaaatggac cacaacctaa actaccaact acatataaag
     3361 aagcattaca acaccttata gaacaagtag aagtaaatga gaaattacaa ctagaaagta
     3421 aaatgaaaga aaaagtaata aaagaactaa aaccaaaggc agattataca gatatgatac
     3481 ttaaaaacaa aggtctagtc actataactc aaatagcaaa ggattatgga atgagtggaa
     3541 aagaaatgaa caaaatactt catgaaagag ggattcaata taaacaaagt ggacaatggc
     3601 ttttatataa acaacatcaa gggaagggat acactcattc agaaacaata gacataacta
     3661 gaagtaatgg aatgcctgat gtaaaaatga caactaagtg gactcaaaaa ggtagattgt
     3721 ttttatatga tttattgaaa acaaataaca tattaccaga tatagaaaaa gaatatagtt
     3781 atcaaacttc aatattaggt tagtactttg aaaactaaat acagaatatt ttggaaagga
     3841 gagattgaat tggaaaataa gaaaatagaa gaaatagaga aaagaataac catgcttgaa
     3901 aatcaattgc aagcacagtt atttagtcag ataacagctc aatcaaatag tgattttata
     3961 gattatatgt accaatgtat agttttgtat ttacatgaga tgaataaaag tgaaaaaaga
     4021 aaaattagtg aaattactaa agaaagaatc agaaaataag aatgataatt acaatataaa
     4081 acctattctt ctagcaggtt ttgaagggtc agttttcttt actgaagtta tcataaagtt
     4141 tatctgtgat gtgtgttgaa gaatttcaga ataaactcca tttactatgc cgtagtagtg
     4201 aattagacaa gggttatgat aacctacttc ttcaacaatc attaatacat cttttccaaa
     4261 gttagtcaat tttagtgcaa tttcatgttc atcatcaagt tctttttcaa attcctctat
     4321 agatttaaga ataattttat attgaaattc agcatagtaa aaatactctg tgttagaagt
     4381 atcagagact gtctttctta tactttcttg taattcttta gatatattgt ttgaaaactg
     4441 cataatacac catccttttg aaaattttgg aatttattcc atgtttatat tataccatgt
     4501 agaactgagg tgaatacaat gttaataggc gacaatataa agcaaatact aagaaaaaga
     4561 gatataaaac cttataaatt ggcaaaagaa ttggatatag atgtaagtgg tttatataaa
     4621 ttactgagaa ataaaagttc taatccaact atagatactc taataaaatt agctgattgt
     4681 ttagatatta cattagacga attggttgga agataaataa ttgaaaagag gggagagcat
     4741 tttcacatgg atatttctga aagcataaca aaacaattta gtgatagctt aaaaagctta
     4801 atagaaatag agataagtaa acaagagaca gatagagtta agagtcaaac tattgaacaa
     4861 aaggtaaagg tactggagcc taaagatata gttgttttaa taaaaagagg ttatccaaat
     4921 tatctaataa cagtagaaga agcaagggga attttaaagt tagatacagc ctttatgcgt
     4981 aggttagtaa gcacaggttt gataaaatca ctagttagag gtgatggtag aaaaatttca
     5041 agatatgaag ttgatgattt tattgaaaga aatcaaggta aaaatttgga tgaactttta
     5101 aaagcagtag agagggggga tgaaattgct gagtcttgat actaataata agaatgtagt
     5161 aactcttaaa aaagatggaa aagttatagc agatatagta tttaaagata ttaaaactgg
     5221 taagaaaata tcggttggaa tattaaataa aaaagtgttg gtaaaataac caacacacaa
     5281 aaaaataaaa aataaaatat aacacaagta aagtataaca caaaaaggag atatttaaaa
     5341 tggttgaagt taatgtaaat gtaaaagtta aagtagaagc accagaattt acaaatgcac
     5401 tattagtagt ggcgaatgct ttaggaggtc ttaatctagg aaaagcaatg cagatagaac
     5461 ctataaatat aacagatatg aaagaagaga agaaagttga agttaaaaaa gctgaaaaga
     5521 ttaaaaaggt agaagtaaaa gaagacgtta aagaagaaat agcagaagca aaggaagaaa
     5581 tagaaaagaa taatgaaaat acaactagtg aagttaaata cacaaaagaa gaggtaagaa
     5641 ctaaggcagc acaagtaagt aagtcaggta agaaggataa gcttaaggag ttatttggtg
     5701 aatttggagc tagtaagtta agtgaagtaa aagaagaaga ttattcagct tttatgaaca
     5761 aattagaaag tttatagggg gtacataaat gccattacaa catgcaagac ttagtgcgag
     5821 tggagcccat agatggcttc actgcactcc tagtataaaa ttagaagaaa actatccacc
     5881 atcaactagt atatatgcag aagaaggaac agttgcacat gaattagcag aagttaaatt
     5941 aatgctagag tatgaaaaaa taagtaaaaa ggcatataat gcaagaatta aaaagataca
     6001 gaaaagtgag tattataact ctgaaatgga ggactatata caatcttatg ttgagaatgt
     6061 agttgaatta gtaaatgata gtaaagctat atgtgatgat gtaatagtga tgttagaaga
     6121 aagactcgat tttagtgagt gggttccaga aggatttgga acaggagacg ttgttgtaat
     6181 atctgatggt atacttcaag ttatagacct aaagtatgga aaaggtttag aagtttcagc
     6241 tatagagaat cctcaactta gactatatgg tttaggggca tataatcagt ttgaaatgct
     6301 atacgatatt gatttaatta aaacaacaat aattcaacct agacttgata acatatcaag
     6361 tgaagaaata gaagttacta aattacttac atgggcagat aatgttaaga agaaggctca
     6421 aatggctttt aatggtgaag gagagtttgt aagtggcagt cattgtggat tttgtagagc
     6481 taaaaatgat tgcagaaaaa gagctgagga taaccttaaa ctagctagaa aatatgattt
     6541 tgctgacaca tttgctctta ataaatatga gatagcagat attttaggtt ttgctaagaa
     6601 tatacaagat tggttaaagg atgttcaaag ttatgcacta gaacaagctg aaaagcatgg
     6661 tgttaaatat ccaggatata agcttgtaga aggcagaagt aatagaaagt atatagatga
     6721 gcaagaagtt gctaaggttt tattaaattc agattatgat gaagaaaaaa tttataagcc
     6781 aaggacctta aaaggaatta gtgatatgga gaaagctata ggtaaaaaga gttttgctaa
     6841 actactaagt gatttaatta taaaaccagt tggaaaagct actttagtag tagaaagtga
     6901 taaaaggtca gaaattaata gtatagattc agcgaaaaaa gattttgaaa tataaatggg
     6961 ggatattaca tgccattaga tgcaggtata aaagataaat tagatgaaag cataagtata
     7021 attttagatt taaaggatag aaaaaagagt atcgaaaaag atttagatta tgaaaaagat
     7081 aatttactag atatgttaaa gagactaaat attacagaat ataactcaac tgaagaaaat
     7141 gcaaaagtta cagtgatgga ttttaaaaga gaaagtttaa taaaggataa agtgatgtca
     7201 actttcctcg aagtaaatga caatcatctt gatagagtgt atataccaga acatataaaa
     7261 gttagtccag tatgctttgt atcagtaaga gcaaaggata attaaaaata tttaattcct
     7321 agtagctata aaaagattta aaaggggata taattttatg agtaatcaag aaataactaa
     7381 actagttgaa aataatttga atcttgtaca ttttagtata aatagatttt ttaagtctta
     7441 tgtagagcaa catccatact tatatgaaga gttttatcaa gaaggatgta ttgggttata
     7501 taaagcagca ttaaattatg atagtaacag aggcaagttt tctacagtgg ctgtttctta
     7561 cataagaata gaaatgttta aagctttgcg ttatggcgaa aaacattata gaaactctat
     7621 agaaggttta gatgtaagtg tatttgaaga tagtaaaaga ccattaaaag atatacatgg
     7681 attttatgaa attcaattta actatgatgt tgattatata agaaaaattg caagaaagtc
     7741 atatttaaaa gatattgata aaatagtaga ttggatttta caagataaaa agatatctga
     7801 tataggagag ttattaaatg tttcttcaac agttatttgt ggaagaatta aattgtttag
     7861 taagcaagtt agagatttag ataaatttgg agaaataact tcaaatataa aatcaatata
     7921 ccaaatataa aaatattagg aggaagaaaa ttatgagtaa ttcagtacaa tcaacaaagg
     7981 tagtaacagg aaaggtaaga ttaagttatt gcaatatctt taaaagcaga gcaatggtag
     8041 aaggtgcaga gcccaagtat tcagtttgta ttttaatacc aaaatcagat aaggtgactt
     8101 taggaagaat aaagaaggct attgatgcag ctaaagaaca aggtaaaact tctaaatggg
     8161 gtggaaaatt accaggtaat ttaaaaacac ctattcgtga tggagacgca gaaagagctg
     8221 acgaagcaga ggaatatgta ggaatgtatt ttttaaatgc aaatagtact caaaagccag
     8281 gaatagttga taaagactta aatgaaatat tagataatac agaggtatat agtggttgtt
     8341 atggaagagt tagtataaac tttttcccat ataatagtgc aggaaataaa ggaataggtt
     8401 gtggcttaca aaatgttcaa aagttagcag atggagaagt acttggagga gctagagcta
     8461 gtgcagaagc agatttctca gatgactttg aatatgaaga tgaagaagag gacttcttaa
     8521 gttaatgagg accttatcaa ttgatataga aacatatagt gatttagata taaaaaaagt
     8581 tggagtctac agatatgtag actctgctaa ttttgagata ctgttatttg cctatgcttt
     8641 tgataatgaa gaggtaaaag ttattgattt agtaaatgat gaagagttac caaaagaagt
     8701 aatagaagct ttaaatgata ataaagttat aaaatcagca tttaatgcta attttgaaag
     8761 aacagcaata agtaaatttt taaatattaa tttaaaacca aatgagtggt catgtacaat
     8821 gataaaggcg ttaacactag gacttccagg aagtttagat agtgtgtcta aggctttaaa
     8881 gtttaatgaa gataaacaga aaatgaaaga aggtaaagca ttaatacaat atttctgtaa
     8941 accttgtaag gctacaaaag ttaacaaggg aagaactaga aatttaccaa tacatgatat
     9001 ggaaaaatgg aataaattta aagaatattg taaacaagat gttgtagttg aaagagaaat
     9061 aagaaacaaa cttagtaagt ataagactac cgaaagagaa attaaattat ggtatttaga
     9121 ccagagaatt aatgatactg gtattaaagt agatacagag ttaatagaga atgcaataga
     9181 atgtgataaa agatatactg aaaaacttac aaaagaagca attaaaataa ctggtctaaa
     9241 taatccaaat agtccagctc aattaaaaaa atggttaagt gataaagtta gctttgagat
     9301 tacaagccta acaaaagaaa gtattccaga aatattaaaa caagttgatg atgaaaatgt
     9361 agttagaact ttagaactta gaaaattaat gtctaaaacc tctataaaga agtatgaggc
     9421 tatgaaatta gctaaaggta atgacaatag agtaagaggt ctactacagt tttatggggc
     9481 taataggact ggtagatggg caggaagatt agtacaagta cagaatttac cacaaaacca
     9541 tatagaagat ttagacctag ctagaaacct attaaaagaa ggtgactttg atttaataga
     9601 gcttttatat gatagtgtac cagatgtctt aagtcaattg ataaggacag cttttatacc
     9661 aagtgaaggt catagattta tagtatcaga ttttagtgca atagaagcta gggttatagc
     9721 ttggcttgct ggtgagaagt ggaggctgga tgtgtttaat tctcatggaa aaatatatga
     9781 agctagtgct agtcagatgt ttaaagttcc aatagaaagt attaaaaaag gttctgaact
     9841 tagacaaaaa ggaaagctag cagaattatc gaatggatat ggtggaagtg taggggcctt
     9901 aatttctatg ggggctatta aaatggggct taaagaagaa gagcttcaac ctattgttac
     9961 tgcatggaga aatgctaatc caaatataac taagttttgg tgggatgtag ataaggcagc
    10021 taaaaaagct ataaaagata ggactatagt agaaattcaa catgggatta aatttattta
    10081 taatccaggc gttttattta tagaactacc aagtagtaga agattatcgt atctaaggcc
    10141 taagatagag ccacatacta catttagtgg agataagata acatatgaag gtatggaaca
    10201 gacaagtaaa caatggaaaa gaatagatac ttatggacct aagttagttg agaatattgt
    10261 tcaagctaca gctagagatt gcttaagaga ggctatgttt aatgtgacag atgcaggtta
    10321 tagcattgta atgcatgtac atgatgagct tgtaattgat gtagataaaa aaggaggttc
    10381 tttagaagaa gttaacagta ttatgggaaa agaaatatct tgggctaaag gtcttcctct
    10441 taaagcagat ggatatgaat gtgattatta taagaaagac taggtgattg atttatggat
    10501 ataaaggcca gtgaaattga acacataaat gtaagacatg atggccaact catgctggcc
    10561 ataggaaaaa ataaattaga aacacattgg aaaaataaaa gtattttatg gtccgaactt
    10621 gtaaatagat taagtaaaac attaagaact caagaaacat atacagaata taggaagatg
    10681 tcaaagactg aaaaagatag agttaaagat gttggtggat ttgtaggtgg aagcttaaaa
    10741 aatggaagac gaaaagcaga gaatattgca aatagaagta ttataacttt agatatagat
    10801 tatgcgaata aagatatatg ggaggatata acattattaa atgattatgc ttgtcttatg
    10861 tattctacgc actcacatac tgagaataat cccagatata gacttgtaat acctttaact
    10921 agaccagtac taccagaaga atatcaagca atttccagga tgatagccga tactatagga
    10981 atagatatgt ttgatgatac tacatatcaa cctcatagac ttatgtattt tccaagtact
    11041 tcaattgatg gagactatat atttaaattt caagatggag agtttttaaa cccaaatgaa
    11101 atactagatt tatatttaga ctggacagat gtaagctatt ggccagaaag ttcgagggag
    11161 aggcaaaagt ttaatacaca attaaaaaaa caacaagacc ctattgaaaa ggctggaatt
    11221 ataggtgcat tttgcaggtc ctatagtata aaagaaacta tagaaacttt cttaaatgaa
    11281 gtatatattc ctggtattga tgaaaccagg tatacatatt cagaaggtag tacaagtggt
    11341 ggagtagtta tttatgatga caagttttca tatagtcatc atggtacaga cccagcaagc
    11401 ggaattttat gcaatgcttt tgatttagtt aggatacata aatttggtga acttgatgaa
    11461 gatgctaaac ccgaaacgcc tgtaaataga ttaccttcat ttactcgaat gagcgaattt
    11521 gcaagcagtg acactaaagt acgaaagact ataggaagag aaaaccttga taaagctaag
    11581 gatgattttg gtgatataga ttttgaagat gatgaatggt taactaggtt agattatgac
    11641 aataagggaa gttataagaa aacaacaaac aatatcttaa tgtttataga aaatgaccca
    11701 tatttgaaag gaaaaatagc ttataatgaa ttttcaaata gagctgtcgt tttaggtaag
    11761 ttaccttgga gaaaagatga taaattaaat gattggaatg atagtgatga ttctgggctt
    11821 agacatcata tagaaacaat ttacaatatc tcatcaccat caaaagtaaa tgatgctcta
    11881 ataattgctt ttgaaaataa tacttttcat cctataaaag attatttaaa ttctttaaag
    11941 tgggatggca ttaagagagt ggatacactt ttaattgact atttaggtgc agaagataac
    12001 tactatacaa ggactataat aagaaaagtt ttagtagcag cagtagcaag aatatttaat
    12061 ccaggaataa agtttgataa tatgatggtt ttatctggtc ctcaaggaat gggaaaaagt
    12121 acttttatta aaaaacttgg tggagactgg tattctgata gtttaactac tgtacaaggt
    12181 aaagaagcgt atgaacaatt acaaggagta tggttgctgg aaatgggtga aatgatggct
    12241 actaaaaaag cagatattga ggcagttaag cattttctaa gcaaatcaga ggatatatat
    12301 agggtcgcat atgggaagag aacctcaaga tttttacgtc aatgtgtagt tataggaaca
    12361 actaatgata aagaattttt aagagataag actgggaata gaaggttttg gccaatagat
    12421 acaggagtaa agaagattaa caagagtata tttaatggcc aacttgataa tgaaagaaat
    12481 caaatttggg cagaagcagt agaattatat aaagccaatg aacagttata tctatcagat
    12541 gaggagaaaa aagaagctga aagacaacaa agaactcact cagaagaaaa tgctaaatct
    12601 ggaattatcg aagagtatct aaataaacct attactaaga attggtatga tttaagtatt
    12661 tcagaaaaga gagaatatat tcatggttca gattttggtg atttaaaaga agggacaata
    12721 ttaagagaaa aaacgtgtgt tatggagata tgggttgagc tatttaatgg agaacctaaa
    12781 caactcacgc ctatcttatc aagagaaatt aatgatatat taaaaggatt agatgattgg
    12841 atatcatata atggaaattt aaggtttggg aaaatatatg gtaggcagag agcttatgta
    12901 cgtaaaagtt agtgtcaaca gaattaaaaa cagagtaaat ttgaaaattt taaaagtgtc
    12961 aacaatgtca acaaaaaaaa tggctttgtt gacgcttatg ttgacaccta agatgcagta
    13021 attgcaatgt atggaatact aacgtctaca atgtctacaa aaattatata taaagtaata
    13081 aaagtaatat taggcatata tgtatatata tatgtatgcc taatatgcat atatacatat
    13141 atatagaaaa tctgtagaca ttgtagacac ctaaatttaa ggaagtgata ttttgttaga
    13201 atcaaaaata gaaaaaagac ttaaaaaaga gattgagttg ttaggtggaa aggctatgaa
    13261 gtttatttca ccaggggagg caggtgtgcc agataggatt gtcttattac cagaaggaca
    13321 tgttatattt gtagaactta aagcaccagg taaaaaacca agtaagcttc aacaatataa
    13381 aatgcgagaa ttaagagaat taggatttaa agttaagtgt gtaagcacat taaaagagat
    13441 agatgatttt atcaaggagg ttaagggatg gaatttaaac cacatccata ccaagaatac
    13501 acaattagaa aaactataga taataataat ataggtttac tattagatat gggattaggt
    13561 aaaacagtct gtgctctaac agctataagt gaattaatgt atgattactt tgatatatca
    13621 aaagttttag ttatagcacc tttaagagtt gcaagagata cctggagtag tgaagtaaaa
    13681 aaatgggagc atctaaagca tctaaaagta tctaaagttc ttggtagtaa attagataga
    13741 gtgagagctt taagtacaga ctcagatatt tatataataa atagagaaat ggtaccctgg
    13801 atagtggatt tttataaaag aaaatggcca tttgatatgg ttgtgataga tgaacttagt
    13861 tcttttaaat caaataaagc acaacgattt aaaagtttaa agaaagtatt gcctttaact
    13921 aaaagagtag ttgggcttac tggtacacca acaccaaata gtttaataga cttatgggca
    13981 cagatgtact tacttgatag aggtgaaaga ctaggtaaaa ctattacagg atataaagaa
    14041 cgatactttg agccaggaca aaaaaattat caaacaggag ctatatataa ttggcagcct
    14101 aaagatggag cagaaaatgc aatatataat aaaataaaag atatttgtat aagcttaaaa
    14161 gcagaagatt atttgaatat gcctagaaaa atagataata aaattgaaat acaccttgat
    14221 agtaaaatac ttaaatatta caaagaatta gaaaaagaga agatattaga attagaaaaa
    14281 gatataataa cagcttcatc agcagctgta gcagcaaata aacttttaca attagctaat
    14341 ggagcaattt atgataatga taaaaatgta aaggaactcc atagagaaaa attagaagct
    14401 ttaaaagaaa ttatagatgt ttcaaatggt aagcctatta tagtttttta taactataaa
    14461 catgattata atcgtttaat gaaagagttt aagagtttga aaccaagaac aatagaaaac
    14521 tcaaaagata tatatgattg gaataatgga agaatacaat tattactttg tcatccagca
    14581 agtacaggac atggacttaa tttacaatca ggtgggagta taattgtttg gtttggatta
    14641 acttggagct tagaactgta tcaacaagct aatgcaaggc tttatagaca gggtcaaagg
    14701 gaaactgtta ttattcacca tctaatctgt aaaggcacca tagatgaaca agtaatggaa
    14761 gctctagaga ataaagataa aggacaaagt gcattacttg aagcagtcaa agcaaaatta
    14821 aaagaatata gggagtgaag gtatgaaagt aaaaagaaaa gatggaactt ataagcccag
    14881 gtaaaatagt aagatgtgct tggtgtggta gaagatttta taagttggat aaatctaaag
    14941 ttaagtattg cagtagaagt tgttcgaaga gagcaagaag gagtgattta agtggataaa
    15001 gaaagtataa aagaaattgt aagagagtta agaaaagaag aaaaagaaaa taaaaaaaga
    15061 gaagtctttc ataatactaa gcttttgtta aagcactata acgatttaaa aagtcatatt
    15121 gcaaattcta ttagtgacat tgaagatgtt aaaagtgatt atgatgattt attatgtcta
    15181 gatatagagg atttatatat attaagtatc aaaagaagta aggcaaaaac cttaattatg
    15241 gtagcacata tagatatggc attagaaaca ttaaaagtaa cacaaaataa gttgcagtcc
    15301 ttagaaaaat acaaagcctt agagttatat ttttttaaag aaaagacata tgaggatata
    15361 gcagagcttt tacattgtgg agtagcaaca gtaagacgtt gggtaaatga aatggttaaa
    15421 gaacttggaa tatacctatt tggagtagat ggtctaaaaa taaaactgtg ataaaatgat
    15481 gatattttat tgttatttta tataagttat aatgatagta tgaaacaagt gtataatata
    15541 ttcccccttt aaaaagacta agctaccccg acttagtctt tttctttttt aaacgaagga
    15601 gaataaaaaa tggatatgat taatctagag gaaatctttt gtaaagcaaa gttgttagat
    15661 aaaaaatata taagtttaaa aataaatata acagaatacc caagtgaaga aataataatt
    15721 attcaaaata caaacttcga aagtaaactt agatattata aagaaatata tgattataaa
    15781 ttaaatcata aatatgtaga tggtataaaa ataatagatt gtacatatgg aaatgatttt
    15841 aaagagatag aattatatat aaataaaaat ttagattaat ttttaataga aaataaagca
    15901 agaaaggttg aaggaactag tagtatttag aaggaggata gtagcatgga gaaaaaagaa
    15961 attgaagaac tgagtaaaat atgtgaacca atactagaat atttgaataa aaaatatact
    16021 ccccataaca gtattgttat ttcaacagat ggaataaaat tattgtctat agaaataggt
    16081 ataccagtaa aacatcaaaa cgactaattt atcagattag tcgtttcttg ttaattaaag
    16141 cttatgataa ggatgtatct tatcaagtct tgataattct gaacctaaag atggagaatt
    16201 taagaagtct tgatattctg tttcacttac atttaaataa gcataagtta ctccattatg
    16261 aaattgaaca tataaagtgt tgttttccca accaacactt ctaattctcc ttgatgaaac
    16321 tggcatcatg ttcatgtgct aacacctcct ttcagtaaaa ttttagcata aatttgtgat
    16381 gaattctgct attgtcgaac gattgttgga ggaataaaat gaaatatgta gaatttatta
    16441 gttatagaat aaaaatcata tatggagtgg aatctttatg gctaatagaa aagttatgta
    16501 ttattgtgtg gaattgaaaa atgtctcaaa tggagagaaa gttagtagtg aacaaatgaa
    16561 agttgagtta aataagatat ttgatgataa atgcatctca catgaggagt taaaacattt
    16621 aattttgaaa gataaaaatg atgcatttga aagaataact atggatataa tatcaaatga
    16681 agaggattat ttatttggaa gagttggaaa attaaaagga aatggtgata ttctgcttag
    16741 aaatattgat acaaataaag ccaatgaaat tgtagaaaaa aataaaagac ctgaaatata
    16801 tacatatttt atgttagatt atgcacatgg cattttaggg tatttaaata atcaatcact
    16861 tccaaaacca gatatgctta aaagaatttt tgatttaaat aatagttgtt ttgaaatgaa
    16921 cataaaaaat gtgataaatc ctcaaacagt tagagagtta atgacgccgg ggtctaagct
    16981 aagtaaaatt gagtatagtt acaaggtacc aagtatatat gtattacaat cattaggatt
    17041 aagtagagaa actgttgcag atttagaaaa tacagactac caaactgtta caatacaaat
    17101 agcgagtgaa tcgagacaga aacttacaga aaaaagtgaa acaataaaaa agctaattaa
    17161 acattttagt acgaataaga atttggaaaa taaagtgttt aaaggtaaag ctcctaatga
    17221 tgctatgcga aattataaat ttgatgtaga taattatagt ttgattatag atgttcctag
    17281 agataaaata gagggtggta aaacaaaaat atatacatta gatgaaataa cagaagaagt
    17341 gaaaattcgt atgaaagaaa aatatataaa taacagaaag catatattaa gactatgtgg
    17401 gatagaataa tatagattcc acttaaggag tgtgaaatac atgaaaaata aacaagaaac
    17461 agtaaagaaa aaaagatttt tatttatgct tgtgctattt tcatgtgtat actatttttt
    17521 aattgtaaag ggaaaaataa atttatcaat tttaacaaat acaacagatt taagtaatca
    17581 ttttaatttt attactgtaa attcagtatt tgtaggtttt ttatttagtt ctctatcttt
    17641 aattttaggg ttgagtggaa ctagctctat aattaggctt gaaaaagcag gctttatgga
    17701 gaatatatat agaaatataa gtatgggtat ttttc
//
LOCUS       DAGSHT010000059        17486 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGSHT010000059 DAGSHT010000000
VERSION     DAGSHT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.061"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            423..1124
                     /locus_tag="MJB72_001815"
     CDS             423..1124
                     /locus_tag="MJB72_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBY8262926.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILL"
     gene            1150..1806
                     /locus_tag="MJB72_001816"
     CDS             1150..1806
                     /locus_tag="MJB72_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262927.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            1911..3551
                     /locus_tag="MJB72_001817"
     CDS             1911..3551
                     /locus_tag="MJB72_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBY8262928.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3909..5231
                     /locus_tag="MJB72_001818"
     CDS             3909..5231
                     /locus_tag="MJB72_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262929.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKLDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5428..6435
                     /locus_tag="MJB72_001819"
     CDS             5428..6435
                     /locus_tag="MJB72_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBY8262930.1"
                     /translation="MGKYQYSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6473..7885
                     /locus_tag="MJB72_001820"
     CDS             6473..7885
                     /locus_tag="MJB72_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBY8262931.1"
                     /translation="MASTNKNNATSKEEEKEVSTSVLESCTVKEKKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            8029..9294
                     /locus_tag="MJB72_001821"
     CDS             8029..9294
                     /locus_tag="MJB72_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8262932.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9336..10934
                     /locus_tag="MJB72_001822"
     CDS             9336..10934
                     /locus_tag="MJB72_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBY8262933.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            10969..11991
                     /locus_tag="MJB72_001823"
     CDS             10969..11991
                     /locus_tag="MJB72_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY8262934.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(12468..13871)
                     /locus_tag="MJB72_001824"
     CDS             complement(12468..13871)
                     /locus_tag="MJB72_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8262935.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            15044..15553
                     /locus_tag="MJB72_001825"
     CDS             15044..15553
                     /locus_tag="MJB72_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBY8262936.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            15581..15775
                     /gene="rpmI"
                     /locus_tag="MJB72_001826"
     CDS             15581..15775
                     /gene="rpmI"
                     /locus_tag="MJB72_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBY8262937.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            15831..16187
                     /gene="rplT"
                     /locus_tag="MJB72_001827"
     CDS             15831..16187
                     /gene="rplT"
                     /locus_tag="MJB72_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBY8262938.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            16497..17042
                     /locus_tag="MJB72_001828"
     CDS             16497..17042
                     /locus_tag="MJB72_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262939.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            17044..17135
                     /locus_tag="MJB72_001829"
                     /pseudo
     CDS             17044..17135
                     /locus_tag="MJB72_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
BASE COUNT         6408 a         1982 c         3246 g         5850 t
ORIGIN      
        1 aagtaggctt ttaagaaaaa acaatgaaac ttaaatgaga attaagagtt agaaatgtca
       61 ttattttaga tagagatgaa cataaataaa cttaaaaaat ataaattata aaaataataa
      121 ttttttttaa aataataaag ttttttgatt aaaattttta tattgaaatg tcaaaataat
      181 aaaagacctt ataagttaat tacaggttaa aaaaataata tgtgaatttt ctgatattta
      241 ctaattatga ataaaatatt taagtattaa tgtttttagt gagactttaa gacagataat
      301 ctcctattaa agatactata tcttagataa aataaattaa aacaatataa aaacataaca
      361 agattacaga aagaaagctc acaataaaat gctgaaaaat ttattttaag gaggaataaa
      421 gtatgaagtt tcaagaaatg attaatagcc caggtctttg gattgtttct agttttttag
      481 taattatttc agttgttcaa gcaatcgtat ttatgaaaga ggcactaaaa gaggcttcaa
      541 atcttggtat tgaaaggaaa ggaattaaag cagcaattcg ctcagctagt gttacagcca
      601 ttggaccttc tctatctcct gttattacat tattatcact ggtagctgtg attggcgctc
      661 ctacgacttg gatgaggctc tgtgatgtcg gagcagctag aacagagctt ggagtaatat
      721 ctcttacatc caatttatct ggggtagaag taggtagtgc tgcatttgga gcagaagctt
      781 tttcgtatgc actatggggg atggctttaa ataacttagg ttggctattt gttgtattta
      841 ttttgggtca tagaatgcgt ggaattgttg aaaagatgaa catgaagtat aatcctactt
      901 gggttaaaaa actacttgca ggagctacag ttggattatt tgcttacctt ctttcaaatc
      961 aaataaaaac atttgaaata ccaaaactta caccagcaat tatttcagct atagttatgc
     1021 tagtattgac aactttattt aaaaagaatc aacgattaca agaattatcc ttaggtattg
     1081 ccatgttaat tggtatgttt ggtacacaga ttatattatt gtgattaaaa cggataagag
     1141 gtgataatta tgtatgataa aaagatgata aaaataggga gaatttctat aggtcttgca
     1201 attattgcaa actttattcc cgctatctat gtaggtattc gctttggtca gatgccaatg
     1261 ctttctgtta tatttcaaat ttgggggtta gtcgctgcga cttatggggt tagttggatt
     1321 gttcaaccta tagcttatta tcctacacta ggagctccag gttcttatat aggttggctt
     1381 gcaggttctg ttggagatat acgtatgcct gcagcatcaa tggcacagaa aattgcaggt
     1441 gtagaagcag gaactcatga gggtgaagtt gttggaacaa ttggtacatg ttgttcaatc
     1501 tttgtatcag cttctatgat taccattttt accattgtag gctataaagt aattcctttg
     1561 ttacctgaat ttgttactaa ttcatttaat tatatacttc cagcattatt tggagcaatt
     1621 tatgttgata ttgcacgtaa ggatattcga gctggtggat gtacgattgc tgtagcgtta
     1681 gcaattatgt actttggtga aaaaattggt ataccaggtg gattattaac attgtttatt
     1741 gtgttaggtg ggatacttat cacaagggtc ttctttgtaa ttgataacaa taaaacaaaa
     1801 gaataggatt gcttattaag gagaaagact agaattattt gttgagaaaa aagaatagga
     1861 ttgtttatta agaaatatga atataaataa gtcttgaaag gagaaaaagc atgtttgatg
     1921 ttaaaattgt caatggaata atcgttgatg gtacaggaaa ttcaagatat aaaagtgatg
     1981 tagggattgt aggagacaaa atcatggcta ttggagattt aagtcaaaaa gaggctaaag
     2041 agactataga cgcaactgga aaatttgtat cacctggttt tatagatttc catactcatt
     2101 ctgatttatc tcttgtgtat gataaataca caagaagccg tatccataca ggtgtaacaa
     2161 cagatgttat tgcaaactgt ggtataggag ttgctccgat aagagaagag aagaagcaag
     2221 agttaattga ttatcttgga actaggataa ttggtacaat tccaacaaaa ttagagcttc
     2281 attggaatac aatgcaagaa tactttgatt acttaactga aaattctcca gcagtcaatg
     2341 ttgtagctta tgtagcacaa ggacctgtac gtatcaatga aatgggattt tcaaaggaac
     2401 cagctacttt agagcaactt aaaaatatga agttggaagt tagaaaagct atggaagctg
     2461 gatgtgtagc tctttcatca ggattagttt atttaccagg agcatacaca aagaaagaag
     2521 aaatggctga attatgtaag gaattgatac catacaatgg atactatata tctcatatta
     2581 gagatgaagg tgatgaagaa atggaagctc ttgatgaagc aatttatata gctaagactg
     2641 caggagttcc tctacatgtt tctcatttaa aagttatggg tcataagaat tttggaacta
     2701 ttgatgaggt atttaaaaag ctagatgaag ctgaggcaga tggtttggaa gtaacttttg
     2761 attgttaccc atatacagct ggaatgacat ctttgggtgc acttttacct ccttgggctt
     2821 ttgaaggcgg tgtagaaaat atggtaaaac gattagaagt acaagaaaat agagacagaa
     2881 ttataaaaga gttggaggaa gggattcctg gatggcaatg tttctaccaa ttagcaggtg
     2941 ggtggaacgg tgttgtacta gcctctgtaa tgactgaagc aaataaatat gtagagggta
     3001 aaactttaat ggaggtagcc aaaatcaatg gtgaaaatcc atttgatacg ttctttaggc
     3061 ttttgattga agaaaaaagt aaaattcaag tagttgttca tacacaaggt caagaggata
     3121 cagataaggt tgtttgccat ccaaagtcat gtataggttc tgatagtatg gacctttcta
     3181 cagaaggttt actttctctt ggaaagcctc atcctcgtgc atttggtact tttggacgaa
     3241 ttttctctta ctatgttaga gaaaaaggta tgttgacttt tgaggaagca gttaaaaaga
     3301 taacttatct ttcagcaaaa cgcttaggca tttataaaga aaggggtcta cttaaagaaa
     3361 attattttgc tgacattgtg gtatttgacc cagatacaat agaagataaa gcgacttata
     3421 gtaatcctaa acagtataca gtgggagtgg aatatgtgtt ggtgaatggt aaaattgcat
     3481 tggcaggagg aaagcaaact gatgtatgtg ctggacgtgt aattaaaaat cctcttagca
     3541 tagcaaaata gaatacatta agaactttta taaaaatagt ctttctttga atattaagga
     3601 aggctatttt tgttaaatta ataaagatta ctctacttgt gttttctaaa gaatttaact
     3661 ttaagtagaa aaaacaatat attaattgta aaatgtaatt aatattaatg aataagattt
     3721 aaatacaaaa atttaaatac aaaaatgttt caacagatag tcgtaattaa aaaacttaaa
     3781 gtaacttttt atattaggta taattgatta ttcaaaattc tatgaatcag gagtcctgtg
     3841 gctttagtca tggtaggttt agaaaatgtt ttaagagata atagtatcat taggaaagga
     3901 gagacgaaat gaaaatactt tatgtaggac caggtatggt aatcgataaa atactagcag
     3961 taagtaaaaa gaattttcct aatattgaag cagattttct tagatatgat agatatattg
     4021 aagtacctga tttattggaa aaatatagag gtagagtgga tgcagttctt tttacaggta
     4081 agtcgccatt taaatttttt gaaaaaagac aaaaagattt tgtattgtct gattatattc
     4141 ctagacatga aacaacattg tacagagtgt tgcttgaaat tacatatttg cttaaattag
     4201 atattagtag acttagtata gatacttatg ataaggctat gatgaagaga ttgtataaag
     4261 aaattggagt tgaatttaag gatgaacata tgttttttgc agagcaaaag tatttagaag
     4321 aggactatat aaagtttgtg cttgaatttc ataaatataa ttatcaaaat aatgatgtat
     4381 gttgttgtat tacaggtctt gaagaggcct atcatataat gaaaaaagat aatattccag
     4441 tagttttagc cttgccttct gaggacgtaa tggtgcaaag tataaaaaac attcagatgc
     4501 gttatattgc aaagaaaaac aatgagaatc agattgttgt tcttgctatt aaactgaaca
     4561 tgccttcaga atattcacta atgaaggaag atgagtacgc ttatctttca caaaggatta
     4621 agattcttga taaactatac catttcaata gccgtataga tggtgttttg gtagagcaga
     4681 gtagaagtga atttatgatt tttacaacta aaaaaatact tgagttagaa acaaagaaat
     4741 acagagatat ttatttgttg gatatgattc gtgaagtttc agttataaac acttatatag
     4801 gaattggtta tggaaaaaca gcaaatgaag ctaaatttaa cgcttatgag agtattaaga
     4861 tggctcaaag tcaaaaaaat gatgcagcat atgtagtttt tgaaaatgga gaaattatgg
     4921 gaccactaaa gccaaatcct actgtcaaag agaaaaatag ttttgatgaa cgtttttatc
     4981 aaattgctac agagacaggg ctaagtgcta atactgtata taaagtattc aataaaataa
     5041 taaatagtgg aaaaactgaa tttacatcaa aggaattagc tactatatgt ggtgtaagta
     5101 ttagaactat ggacaggatt atacttagat tgtgtgattc tggttattgt gaggtagtga
     5161 gtgaacagct tatgagcaag tatggtagac ccagtagaat attacgtttt aaaccttttc
     5221 tgttgtttta aatagatata agtcaaaaat taaattttta tgataacatt ttctttacaa
     5281 ttttttagat atatgttaat atttagaaaa atgcatatgt attcataaaa taaacttaaa
     5341 acagctgaaa aatcattaaa aaggaaatcg tatgcatgaa atgagaaagt taatttataa
     5401 cttaaataaa tctgggagga gataattatg ggaaaatatc agtattcaaa aaaagttgta
     5461 agaaattatt ttgaagcatt agaaaatgct actggggatg aggttgaaaa tgtcttaaag
     5521 caacatatga aatcagatta taactggaaa ggtgtgtatc catttcgtga acaagaaggc
     5581 actgaaaatg tagcagatgt attttggaag cctttaaaaa aatcattgag taatatgcaa
     5641 agacgacaag atattttcat tgcaggaaca aatgagattg acaatagtga atgggtaatg
     5701 agtatggggc attttatggg actttttgat aacgattggc ttggcattaa acatactcgt
     5761 aaaatgatta gtttaagata tgcagaattt aactgtgttc aagatggtaa aattgctaaa
     5821 actggattat ttgtagatat aattgggttt atgattcaag caggagttaa tccattgcca
     5881 ccacaaacag gttcatattt tgtatatcca gggccaattg accataatgg attacttttt
     5941 gaagatgcag atgaagttga aggagtaaaa actttagcat tagtaaataa aatggtagat
     6001 gatttatctg aattaaatga aagtggtgct atgggatgcc caccagaaat acttgaaaaa
     6061 tcttgggcta aagatatgat ttggtatggt ccaggtggaa ttggtgcttc ttatactata
     6121 cctagatatc aagagcaaca ccagctacca tttagaaata atttaaaagg aaagactttc
     6181 aatgggcatg tatgtagatt tgcagaaggt aattttgctt gtttctttgg atggcctaat
     6241 ctgactaatg taccatgtgg aggtttttta ggattaccag gtggagaggt taaagcagat
     6301 atgcaagttg ttgatgttta ttatcgtaag ggcgataaac ttcaagaaaa ctgggtgtta
     6361 attgatattc cttattggtt gaaacaacaa gggcttgata ttcttgagag aactaaaagt
     6421 atttttaatg cttagttatg catacgtatt ctaaaacaaa gggggatata aagtggcatc
     6481 taccaacaaa aataatgcaa caagtaaaga ggaagaaaaa gaagtttcta caagtgtgct
     6541 tgaatcatgt acggttaaag aaaaaaaagc taaaatgaat caaactaatt ttagtaatat
     6601 aaatgaaaat caaacaaatg agaataaaac gaacacatat aaaggaaata tagataaagg
     6661 aaatatagat aaaacaaaca caaataaatc cgacataaat aaagttaaca tagatacagt
     6721 ggatacagat aaattaaata caaatactct tactgaaaaa aataaaaaag ttatatttaa
     6781 gggggaagat aatatgtcat ctacaaaaat ttactatgga gattcattag aagtaacgcc
     6841 tgttggagta atggattaca atgatttttc aaaacagaca aaaagggaac aggaaatacc
     6901 tggatttgat tctaagtata gagattttgt agattatatt atgaagatta cacataatat
     6961 ctgggaagaa aaaggtatag gtgttattta tgatacatat cacaataatg ttacaatgca
     7021 ttgtggctct tcaaatttag ttggtattaa agatgttatt tccaatactc ttcaaacatt
     7081 acatgcgttt cctgatagaa gattaattgg tcaaaatgta atatggtcta actttggtgc
     7141 tgatggtttt ttatcttctc accgtgtttt gtctacagca actaacttag gtgatagtaa
     7201 ttttggacca gcaacaggta gaaaaataaa ctttagaact gtaattgatt gtgccacaac
     7261 taacaataga atccacgaag aatggttggt tagagataat ttatggattg ttactcaatt
     7321 aggattaaac cctcaggaag tagcaaagag tatggcaaag gcaagtgaat ctaaagtgct
     7381 tagtttacaa tcaacttatg gtatctgtga atctatggat ggtcaattta tgccaactaa
     7441 gtatcaagca aaagatgatt ctgttggcga aatgatgctt gaaatgacaa gccgtattta
     7501 taattataaa tatataaatg aagttaaaaa atactatcat gataatgcag tagtacattt
     7561 tatatgtgat aaagatttaa atggctatga tgagattcaa ggaatgattg taagtttact
     7621 tgcatcaatt ccaaatggaa gttatgaagt ggaaagagta acttgtaatc aaagagaaaa
     7681 aaatgaaggt tatgatgtat ctgtaagatg gagattacgt ggaataaatg aaggtatagg
     7741 attttttgga cagccttctg gaaagcatat agaagtaatg ggaatcaatc attatcatat
     7801 actacaaggt aaagtaaagg aggagtggat aacttttgat ggtatggatg tattaaaaca
     7861 aatgtatatg ggagtagaag aataaagctg tttagagtat aaatagtttt gaaatgtata
     7921 tagggaagta ttgctttata aaatttaagt ggggaatgta tatgaaaaat agatgtttaa
     7981 acttttagca aaattatgac taaatttaaa agggggaatt ataaaaatat gaaaacaacg
     8041 gttgaaatag aaaatgatat catattagga aaagactatt caaacctaaa aagaggaatt
     8101 gcattttttt caattgcttt aatatacttt ttttattgct acaattttat ggtgggtact
     8161 tttattaaac caacaatgat ttatgcttta gcagatgggg gatttggttt ttcattaaaa
     8221 caaacagaag aaatatttgc agttatgtct tttggtacaa ttccaggtac atttattttt
     8281 ggggtgattt ctacaaaaat tgggaagaag aggactttaa ttagcgttgc tttactgatt
     8341 ggattgacaa catttatacc aatgttatct cctaccaata ttatgttatg gaaaatagca
     8401 cgtttgtgta caggagtagt gttgggaggc gtatttggaa cagctatgcc tttagttgca
     8461 gatatgtttc caagtaaata tcgtggtaag ttagctgcta tattaactgc tttattttca
     8521 ttagcaatga tttttggtgg gaaagtatat ggcgttttag gtgatgcaaa ttggcaaata
     8581 ttgatgtata ctgcgattat accacctata gttggagcta tcttagcaat cttctttgtg
     8641 ccagatgatt tggagtatac aaaggaatta gttaaaaagg gaaaagaaac tggtgaaaag
     8701 attagctata taagtatgta taagggaaaa tacttatgga ttggattggg aacgattctt
     8761 ttatcaggtg caaattttac agcatattca gcattttcaa ataatgcaac tacataccta
     8821 gtgacaggaa taggaatgtc agcagcagtg gctgggtcta tatatagttt acaaggaatt
     8881 ggtcaattag taggatattt attctggggt tcaattgcag ataaatttgg aagaaaaata
     8941 ccttttatag gtatggcatt agcagcagta tttgttttta tatatacaaa attaggtgga
     9001 gataatacaa caacattcta tatgatatca gtattactag gtgttgctgt tggatattct
     9061 ggagcatggg gagcatacta cacagagttg ttcccaagta aatatcgttc gttgtcatct
     9121 ggtatgtcat ttaatggagg aaggattata tctacgtttg caatacctgc tatagctggt
     9181 acagcttctg gtactttagg tatgatgact atcttttaca tatcaatcgc agtctttgta
     9241 gcaggtgcta ttgtatggtt attcttaccg gagacgttaa ataataaaaa ataaaatact
     9301 aggaatttat tttgttactt agaaaggggt ggcttatgat ttcatcaatt gatgtatgta
     9361 ttataatagg atatttgcta ttaatgttag ttattggtta ttattcagga aaagataata
     9421 aaaatcaaga agattatttc ttggcaggta ggtcaatgcc atggattcca attgctttat
     9481 cagttgcagc tactatgatt agtgcaaatg gctttatagg tggtcctgga tgggcatatg
     9541 tagatggaat gtatccagta atggttaata ttacagtacc tttagcaata ttttttgcac
     9601 tgagcattac caccccagtt atctataaat taaaaataac ctctatatat gaatatatgg
     9661 gacttcgttt aggaaattat tcaagaggat taactattat tcaatttttt attaattcat
     9721 tgattcaagc tagttctatg gtatatattc cagtgcttat tattcaaatg attactggct
     9781 ggtcacttta tgctttagtc ccaatagttg tacttgtatc aattatatac actctattag
     9841 gtgggattaa ggcagttata tggactgata gtattcaaat gatagtagta ataagtgctg
     9901 tatttatagt aattattgta gccttaaaag gaatgaatct aagtttcttt gatacattac
     9961 aaattgcaca acagagtgga aaattaaata catttgattt cagtagggat atttcagtga
    10021 caaatacttt ttgggctact ttaattggtg gaactgtaat gtggataaga tacttttgtt
    10081 ttgaccaatc tcaagtacaa agagttttaa catctaaatc tttaaaatgt gcaaaaaatt
    10141 cttttgtagt tagtgcattt gttatgaatt tagtttacta ctttatgcta ttggtaggag
    10201 taattttatt tgttttttat gggggaagga catttgaaac ttcaaatgaa ataatgatta
    10261 cttttatttt aaatgaactt ccagtaggag ccattggaat tattattgca ggtgtgtttg
    10321 cagcagcaat gtctagtatc gactctatat taaacagtat gacaactgtt tttacgaagg
    10381 atatttatga gttttatttc aaaaaagata acaaagaatc atcattaaaa gttacaatga
    10441 ttataactgt agttatagga gttgttatga caggttttat tattatggga tttggaggaa
    10501 gcatcaaatc aattcttgac cttgttggta actatatctc atactttgct ggaccagcta
    10561 caggtgcatt tgtactagca atgtttacat ataaagctaa tgataaagga gtatctattg
    10621 gttttattgt agggttagtt ttaggatttt ttatttctaa taaatataat gtgagttggt
    10681 tgtggaatcc agcaataggt gctacaataa cattgatatt tggatatatt tttagtgtca
    10741 ttttaaagaa aaatcataat gcagatgata ttaaaaaata cactgcattt ggtatgagaa
    10801 atcaccttat ttctcaagga atggataaag tagatggagt atctatagtg ccattttctt
    10861 taggtaaaca agaattaatt gtattagctt tttttattat ccaatatatt atattatttt
    10921 tgatacaatt ttaagaagaa atactagata tttaaggaga aatactaaat gaatcaaaag
    10981 aaagttcatt ataataaatc ggctacctca aaagatgttg caagattggc aggagtatct
    11041 caatcatctg tttcaagagc atttggtagt gcaaatggaa agggcgtaaa acctgaagta
    11101 agagaaagaa tttttcgtgt agcaaatgaa ataggatatg ttcctaattt ggtagcacgg
    11161 ggaatgatta gtggaaaaac taatgttata ggattgatag ttggtgatag tctaggtcca
    11221 ttttacaata gaattatcaa tttatttgta gaaaaaattc aagagatagg taaacagtgt
    11281 ctcgttttta aagttcctag acaacaaagt atagatagta taatttcaaa agtaattcag
    11341 tttcaagtag aagctgtgat tattacagct tcggccatga ataaggtgat ggctgagact
    11401 tgtgaagaaa acaatattcc tgtgattctt tttaatcgtt ttatacctgg tataaaaata
    11461 agtacagtct atgtagaccc aattgaaggt gggggaatgg ctgctgaata tctgcttaag
    11521 aaaggtcata agaatatagg atatattcaa tttacgagag aaacaagtga agaaatggaa
    11581 aagaaaattg gattttactc taagttaaga caaagtggga ttcataacct taaagaagaa
    11641 tctgcaaact atgattatga ttctggatat gaagcaggta agagaatgtt agcattatca
    11701 aatcctccaa cagcaatttt ttgcacaagt gacttgatag ctattggtgt aatggatgct
    11761 gcaaggtttg agtacaagtt gagggtgcca gaggatttat cagtgatagg atatgatgat
    11821 attcaaatgg cttcatggaa gagttataat ttaacgacga ttagacagcc actagatttg
    11881 ttgattgaaa aaacaattaa catacttaag tccttattaa atgaagaaaa tccagagtca
    11941 gtcgttgaaa tgttaaaacc tgaattgatt gaaagaggtt ctacacttta agtggtagat
    12001 atttaattaa gtggtagata tttaattaaa tggcagatat ttaatcaagt gatagatacc
    12061 taattaagtg atagatattt aatcaagtga tagataccta attaagttgt agatatctga
    12121 taagaaattt ttaagttttc aatcgttcta aattgtgagt ataaaaatct actaaattta
    12181 gttatgtaaa atataaatta taagtgtaga aactaggcta ctagctttta taccacttca
    12241 aacattactg gaggtagtta agtttacagc agtttagttt tatgtagata taatattctg
    12301 taaatagaca aaattaaaat atgtaattaa aatatgtaag tgtctcaaaa tgaaattttt
    12361 agtttatgag gcactttttt gtataaaatc aaatgtattt ttataacttt aacaataaaa
    12421 agaggcttat ctctattttg agacagcctc aaatttttaa aactatatta gtttacagct
    12481 tcttttccta tcaaatcttt taactcttca tgagtcatag tttctttttc aaataagaat
    12541 ttagaaacag aatgtaaatc ttcaagatta tcctttaaca tctctaaagt ctctttataa
    12601 caaatttcaa ctatacgatt tgcttccttt tgaaccttat cacataagaa agtcttatca
    12661 tttccatcaa tagtcataaa tcctaagtta ctcataccat attcacaaac catttggtta
    12721 gctatttcag tgactttctt taagtcgtcc tttgcaccag tagatttatg attaaatatt
    12781 aattcttctg aagcctttcc agcaagtaat atttttattt ttccaatcaa ctcatcctta
    12841 gtataaatat atctatcttc atcaggtaat tgaagaacat aacctaatgc ttgtcctcta
    12901 ggaactatag atattttttg tataggacaa attcctacta tatcacttac aagagcatgt
    12961 ccagcttcat gatatgctac tatttttttc tctttttcaa ccaatgcaga gtttttagat
    13021 tctagccctg caataactct ttcaagtgcc ttgtcaaaat gttctgatgt aatacactca
    13081 ctgtcatctc taactgcaaa tatagctgct tcatttgcta tatttgaaag atgagcccca
    13141 ttgaaaccat gagttttttt agctaaaagt tctagattta cagatttgtc tataggtttg
    13201 tcatctgtat gtacctttag aatttcaaat ctagtgtgat agtttggtgc acctatatga
    13261 atgtgtctgt caaatcttcc tggtctaagt aaagcttcat ctaataagtc aagtctattg
    13321 gtagcaccta ttattaatac attggaatct ttattaaatc catccatttc aactaaaagt
    13381 tggtttagtg tttggtcttt ttcgttgtta ctttcaagat gtctctttgc tcctacagca
    13441 tctatttcgt ctataaatat tatacttgga gcttcttttc tagctttttc aaatagagtt
    13501 ctaactcttt ttgctcccac acctacatat ttttcaacga actctgaacc agtaacattg
    13561 aaaaatgatg agtttgtttc accagctaca gcagatgcaa gtagggtttt acctgttcct
    13621 ggaggaccat aaaataatac tcctttaggt atttttgcac ccattttttg atatttttgt
    13681 ggtgacttca taaaatcgac tatttcaaat agttcttctt ttacttcgtc taatccagca
    13741 acgtctctaa atgtagtctt tggttttgta gaaagtgaat catctgtttg attatctctt
    13801 tccatttttg tctgtgcaaa gacaggagct ggattgttga ttttcttaaa aaagttgttt
    13861 agcattttca tattcacacc tcaggtaatt aattattgtt aatcaatttt atattttcca
    13921 tatttactat tttttattat ttatttttca tggaaaaaat atacatgtta tctaaataaa
    13981 aataaatttg taagatgtat gaaaaaatga atttatgtat atagagattt ctacttatgt
    14041 attgaataaa gtattacaaa ttataaatta tgtattgatt atatattgag taaagtatta
    14101 taaagcatgt attgattaag tatttattaa gtattgaata aaatattaca aatttagttg
    14161 aaatgaaaat gataaaaaat gttgtactat attttacaaa tcatataaat agagaaattg
    14221 ttgtagcgca aagtagtagg tagtatatta gaatataatt ataggatttg ttgtgtagta
    14281 tagtagcata ttagaatata atataggatt tgttatgtag tatagtagca tattagaata
    14341 taatatagga tttgttatgt agtatagtag catattagaa tataatatag gatttgttat
    14401 gtagtatagt agcatattag aatataatgt aggacttgtt atgtagtata gtaacatatt
    14461 agaatataat gtaggatttg ttatgtagta tagtagcata ttagaatata atgtaggact
    14521 tgttatgtag tatagtaaca tattagaata taatgtagga tttgttatgt agtataatag
    14581 tatattagag tacaatatag aatttgttat gtagtatagt agtatattag aatacaatat
    14641 agaatttgtt gggtagtata gtgttatatt agaatacaat ataggatttg ttatgtagaa
    14701 tatatagagt catgtagaat atacatatat aaaaggattt tgggtataaa tttaaaaatt
    14761 aatagcaaaa tgagtagtat agacatataa ttagtagtag gaagtaatta aatttttcct
    14821 aaacaaaaaa aatagttgca aataatttga ttaagtgata tagtaaataa ggtaagaata
    14881 aaacaaagaa gaagtccgct tctcacctta cgccggaggt tagtaaggta aatataagtt
    14941 aaagcttaat tttaatttaa gtttgtttaa atttggtaac ttatattgcg gatgattacg
    15001 tcatccgctt tttattttat aaaaattttg gaggtgtcct acaattagca aagaattagc
    15061 aatcaatgaa caaataaaag ataaggaaat aagagtcctt tcaccaacag gtgaacaact
    15121 tggagtaatg cctactaaag aagctcaagc aatggctaat agcaaaaact tagatttggt
    15181 tcaaatctcg ccgaatgcaa atccaccagt atgtaagata atggattatg gaaagtttag
    15241 atatgagcaa gcgagaaaag agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa
    15301 agaagtaaga cttagaccag gtattgaaca aaatgattta aatacgaaag ctaataatgc
    15361 tataaagttc cttaaaaaag gggataaagt caaagttgaa cttagattta gaggaagaga
    15421 attaggacat aaagacatag gtaaagaagt tatgcttaag tttttagata taataaaaga
    15481 gtttggagaa ccaacaaaag cccctgcatt tgagggtaat aatatggttg taattataga
    15541 tccaaaaaaa tagatgatta actgagagga ggaatttgtc atgcctaaaa tgaaaactca
    15601 tagaggtgca gcaaagagat taaagaaaac aggaactgga aagttaaaga gagctaaagc
    15661 ttttaaaaaa catatattaa ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc
    15721 aactttagta agtgatggag atgctaagag aatagcacaa ttattaccat actaagatac
    15781 agattgtaaa taataactat agaagaggga tataaaagga ggtcataacg atggcaagag
    15841 ttaaaaaagc tatgaacgct cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat
    15901 tcagaggatc tagaagcaaa ttatatagac cagcaaatac attcgtaatg aaagcattaa
    15961 aaaatgctta tataggaaga aagttaaaga agagagactt cagaaaactt tggatacaaa
    16021 gaataaatgc agctgctaga atgaatggaa tatcttattc aagattaatg aatggattaa
    16081 aattatctgg tgttgaagtt aacagaaaaa tgttatctga aatggctata caagacccag
    16141 aaggatttgc aaaattagca gaagttgcta aagcaaaatt agcttagtat tgaatcatga
    16201 gatgtaaagt ctttgagtta tataaataat tcaaggactt ttttattttt tgaaaaatac
    16261 ttattagtgt catgtacaac caattttata atgataaata aaataagcaa tacaatagaa
    16321 ttttatgaga tttattataa gaattgttac aattatttat gatagctata aaaagtcata
    16381 gtaaaaatga tattgatgta tgttgatata tgtataaagt ttccaatcaa tcttgaatta
    16441 atattatcaa catgataata ttatcttatt aataatataa ataaggggga cataatatga
    16501 atgaaaaact aatggattgt tttgtaaacc caattaaatg caagttattg attgaaattt
    16561 tttcaaataa tcgaactaca gctaaacaat taggagaaaa atttcaagat atatcaaagg
    16621 caacacttta tcgttacctt aataaaatgt tgaaagatga tattataaaa gtggttgaag
    16681 aaaataaaat aaggggaact gttgaaaaag tatatgctat gaagttagat atagctgatg
    16741 atgtgaacaa aattatagat gagaactctg gagaagaata tgctcaactg tttactcaat
    16801 atatgatggg atttttaaga gaattttatg aatacgctga aagaaaggaa aaggatatcc
    16861 taaaagatgg ttcaggattt tctgtctgtc cagtatatgc tagtattgat gagatagttg
    16921 aagtgagtaa aaaaattgct agtatactag aacctctaag aaataactca catgaagaag
    16981 gtagaagaat gcattctata ggagtgataa ttactccacc taaaaaggat gaagggaaat
    17041 aaatctgatt tacatggggt agagccaatt tatacaggga atcgttttat gatttacata
    17101 tgtatccaga gcaaagtatc tctgcttgga ttgtacttca ttgtaccatt tcatccaatg
    17161 acttggagtg gtacaatgaa gttttttaat ttttcatatt aatgctaaag aaatgaactt
    17221 tagaccacat atgcaattag gttgcattat acaattagac cacatataca aaataaaaat
    17281 cagtattttt atagcagata aattgactgt cattcttctt aaaaatcatc tattagttat
    17341 tgttagcata ttgaaagaaa ttatagtaat ctattgaata aatgaatatt gcctataatc
    17401 atataaactg ctatagttta aaataaagca ggacgttaaa ttttatagta atctaaaaat
    17461 ttaagagggg gattttatga aaataa
//
LOCUS       DAGSHT010000060        16823 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGSHT010000060 DAGSHT010000000
VERSION     DAGSHT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.062"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..343
                     /locus_tag="MJB72_001830"
     CDS             <1..343
                     /locus_tag="MJB72_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262940.1"
                     /translation="NVREKEFLKAIKVRVKIKNPWIGIDIEDYMDKSNNNKIDDKTFN
                     NYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQNIIENIKKNFDEK
                     NLVQVYKYRGV"
     gene            363..782
                     /locus_tag="MJB72_001831"
     CDS             363..782
                     /locus_tag="MJB72_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262941.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            772..1296
                     /locus_tag="MJB72_001832"
     CDS             772..1296
                     /locus_tag="MJB72_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262942.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            1608..2165
                     /gene="efp"
                     /locus_tag="MJB72_001833"
     CDS             1608..2165
                     /gene="efp"
                     /locus_tag="MJB72_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBY8262943.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            2257..2712
                     /locus_tag="MJB72_001834"
     CDS             2257..2712
                     /locus_tag="MJB72_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY8262944.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            2875..3585
                     /locus_tag="MJB72_001835"
     CDS             2875..3585
                     /locus_tag="MJB72_001835"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBY8262945.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            3639..4709
                     /locus_tag="MJB72_001836"
     CDS             3639..4709
                     /locus_tag="MJB72_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8262946.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            4737..8291
                     /gene="smc"
                     /locus_tag="MJB72_001837"
     CDS             4737..8291
                     /gene="smc"
                     /locus_tag="MJB72_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBY8262947.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            8318..9598
                     /gene="ftsY"
                     /locus_tag="MJB72_001838"
     CDS             8318..9598
                     /gene="ftsY"
                     /locus_tag="MJB72_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBY8262948.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            9701..10069
                     /locus_tag="MJB72_001839"
     CDS             9701..10069
                     /locus_tag="MJB72_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBY8262949.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            10066..11418
                     /gene="ffh"
                     /locus_tag="MJB72_001840"
     CDS             10066..11418
                     /gene="ffh"
                     /locus_tag="MJB72_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBY8262950.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            11456..11728
                     /gene="rpsP"
                     /locus_tag="MJB72_001841"
     CDS             11456..11728
                     /gene="rpsP"
                     /locus_tag="MJB72_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBY8262951.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            11793..12020
                     /locus_tag="MJB72_001842"
     CDS             11793..12020
                     /locus_tag="MJB72_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBY8262952.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            12128..12643
                     /gene="rimM"
                     /locus_tag="MJB72_001843"
     CDS             12128..12643
                     /gene="rimM"
                     /locus_tag="MJB72_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBY8262953.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            12652..13347
                     /gene="trmD"
                     /locus_tag="MJB72_001844"
     CDS             12652..13347
                     /gene="trmD"
                     /locus_tag="MJB72_001844"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBY8262954.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            13532..13882
                     /gene="rplS"
                     /locus_tag="MJB72_001845"
     CDS             13532..13882
                     /gene="rplS"
                     /locus_tag="MJB72_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBY8262955.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            14203..15099
                     /gene="ylqF"
                     /locus_tag="MJB72_001846"
     CDS             14203..15099
                     /gene="ylqF"
                     /locus_tag="MJB72_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBY8262956.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            15488..16780
                     /gene="brnQ"
                     /locus_tag="MJB72_001847"
     CDS             15488..16780
                     /gene="brnQ"
                     /locus_tag="MJB72_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY8262957.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7173 a         1591 c         3171 g         4888 t
ORIGIN      
        1 taatgttaga gaaaaagaat ttttaaaagc aataaaagta agagtgaaaa tcaaaaatcc
       61 atggatagga attgacattg aagattatat ggataaatct aataacaata aaattgatga
      121 caaaacattc aataattata aagcatctga aaataatgaa attctaaatc aatttaaaaa
      181 tgaaagtgaa aatataagga atcaagaggt tggtggattt acagaagaag atgaagaaga
      241 tgaagaatat gaagaagaac ttaaacaaaa cataattgaa aatataaaga agaattttga
      301 tgaaaaaaac ctagtacagg tatataagta taggggtgta taactttgaa aaaagaagga
      361 atgtgactgt aatagaatta atagtatcta tcagtataat actcttgata gttacattat
      421 cttttccaaa agataatctt gaaaatcata aaataaattt atttgcacgt caactttgtt
      481 cagatataag gtatgttagg cgaattaata tgtttagcaa ctacaataca tatatatatt
      541 atataaatca aggtaaacac gaaggttatg tattgagaaa agatggtaaa aacattaaat
      601 caataatgct acctaaaaat gcaaaaatag ctaggagtat tgaaataata aagtttaaat
      661 ctgatgggtc gcctcaaaag gcgtctacaa tagagatata taataataga ttaagaaaaa
      721 ctataacaat aacacctgta agtggaagag tacttctgaa agagggaaaa tatgaaacat
      781 aaatatggtt acttgctttt ggaaagtgtc gtatcattgt cttcaatggt gataattata
      841 ctagttttat attctatatt tttgtctaca ataaacttaa agttaaaagt agaagataag
      901 atagaactac aacaacaaag tcttgaaatc ataaaatcta tggaaggaat tattagcaat
      961 tctatgggaa taatgaatgt atctaattat gaagaaacat ttaaaaagac aacatctata
     1021 aaatgtagat atttagatga aaataataat gaagaaggca taagtaataa agaaattata
     1081 ttgaatgaaa gacgaaataa gttatttgta aattctttaa atggcgaaag tagtcaagca
     1141 ggtggttatg aaattggaga ttatgtagat gagatgtatg tatcaattac taataatggt
     1201 caatatgtta atattaaatt gaaattatca aaacgtagtc aaaaatatga gactgatttc
     1261 aaaataaaag tatggaattt tagtgaaagt atatagaaat acataaattt aagagaaaga
     1321 agtgacatca ataagtcata taaaatataa ttattgagaa ctatataact aaataatatc
     1381 aatatgtaaa gtgtatagat taagaatata gaggataata ataaaaatat agaataagta
     1441 atttgccaaa tacttgttta tattgtataa cttatgatat tatgatatta gtaaaaattt
     1501 gtaatcctga cttgacagaa ttcaagtaag agatataatt ttaaaggcat agtaatgtaa
     1561 aatttactaa aaaataagaa atttatttta atatggagga acaaaaaatg gtatcagcag
     1621 gtgattttag aaaaggtgtt acatttgaaa aagatggaca accatgttta gtagttgatt
     1681 ttcaacacgt taagccaggt aaaggagctg ctttcgttag aactaaatac aaaaacttaa
     1741 aaacaggagc tataagagaa gaaagtttca atccaagtga aaaattccct aaagctgtta
     1801 tagatacaag acaaatgcaa tatctataca atgatggtga gttatattat tttatggatc
     1861 aagaaaattt tgaacaaata ccattaaact atgagcaagt tgaagatgca attaagttct
     1921 taaaagaaaa tgaagttgct acaataagat tttaccaagg acaaccattc caagtggaag
     1981 cgccaaactt tgcagagcta gaagttacag atacagagcc aggtataaaa ggtgatacag
     2041 caagtaatgt aactaaagca gctacagttg aaactggagc agttgttcaa gttccattat
     2101 ttataaatac tggagataaa gtaaagatag atactagaac aggtgaatat ttatcaagag
     2161 tataaaaatt tatgtatata aaagcttata tgtcttaata atataagata tatgagcttt
     2221 tagtttatat ataaaaaaga aagcgagggg tgttacatga aacatttata tgaagaagtt
     2281 gcatacttaa aaggcctagc agaaggacta gaaataagtg ctgaaagtaa agaagggaaa
     2341 atgatacata aaatagttga tgcattagaa gtatttgcag atgctattgt aacattggat
     2401 gaagaacaag aagaacttca agattttgtt gaatctatag atgaagattt agcagattta
     2461 gaagaagata ttgctgacat ggaagaagat ctttatgaag aagatgacga tgaggatgat
     2521 gaggacttca gctatataga aatggaatgc ccaaattgtg gagagttagt agaaatagac
     2581 gaagatttgt tatatgatga tgaagtagat gttgtttgtc ctgattgtaa ggctgtgata
     2641 ttatcatcag aagatgattg tgatgatgat tgtacatgtg gtggttgttc aagttgtgat
     2701 gatagagagt aaaaattcat aagaattaaa actgcctatg atgcctatga gatattttgc
     2761 ttcataggta gttttatttt tatgaggaag ggtattatgt attttggttt tgcaatactt
     2821 ggacaaaaat agtacactag aatttataga ataggatttt ggagggatgt aaatatgaaa
     2881 ataagtaaaa aattactaga taatatacaa agatttgaaa atgttataaa ttataaattt
     2941 aaaaataaag aatatatact agaagctctt actcatagtt catattctaa tgaaaataaa
     3001 aaatataatt ttaacgaaag attggaattt ttaggggatt cggttttagg catagtgata
     3061 agtgattatt tatttaatga agaagctaat ctgcccgaag gggaactaac taaacttaga
     3121 gcaaatatag tttgtgaaga ttcattaagt gaagtagcta atgatataaa tttaggtatt
     3181 cacatgttat taggtagagg agaagaagca acaggaggaa gacataggac ttcaatatta
     3241 gcagacgcat ttgaagctgt aatagctgct atatatcttg atggaggttt tgaaagtgca
     3301 agacagttta tacttcatca tatggaaaat ataatttatg attcaagaaa aggaaatata
     3361 tttagagact acaaaacgca tcttcaagag gtgttacaag ggaatggaga aaataatatc
     3421 tggtacaggc ttatagaaga aaaaggacca gaccataata aaagatttgt aatggaagtt
     3481 ggaattaatg acgatgtact tggtatagga gaaggcaaga gtaaaaaaga agctgaacag
     3541 ttagctgcta aaatagcttt aaagaaaaaa ttatgggaaa aataaatata atatgtaaaa
     3601 atataaaaag aaatatttat attagaagga tgttgtagat gaaaaagaga attataccta
     3661 tatttgttcc tcacaaagga tgtcctcatg attgcatatt ttgtaatcaa aaaaaaataa
     3721 caggtgtgag tactgatgtt actagtgaag atgcaagaaa tataattgag gaatgtttag
     3781 agactataga taaagatgca gatgtagaaa tagcattttt tggtggtagc tttactgcta
     3841 tagatattga tatacaaaaa agtttattat cagttgctaa agaatatgta gaaaaaggtc
     3901 ttgtaaaaga tattagaatg tctacaagac cagattgtat tgatgaagat atattaagta
     3961 tgttaaaaga atataagaca agtataattg aactaggagt acaatcctta gatgaaaaag
     4021 tattattaga tagtataaga ggtcatcaat ctgaaattgt atacaagagt tcaaagatga
     4081 taaaaaatag tggaatcaaa cttggattac agatgatgat tggtcttcca gctgacacag
     4141 aagaaaagtg tatatttaca gcaaaaaaat ttgtagagct aaaaccagat tgtgttagag
     4201 tctatccaac tttggtagta aaagatacag gtcttgaaaa gttaatggaa caaaataaat
     4261 ataatccatt tactcttgaa gagagtgtaa atatagtaaa aaaagttctt gtactgtttt
     4321 atgttaataa tatcaatgta ataagagttg ggcttcaagc tactgatgat atacaaattg
     4381 gaaaagctgt tttagcagga ccatatcatc ctgcatttag agagttggta gaagcagata
     4441 tgataaaaga ttatttagaa tttgttattt tgcaaaataa aaatataaag caaatgttgg
     4501 tcaaatctaa taaaaagaat atttctaaaa tcataggaaa taaaaaaaca aatgttaaat
     4561 atatggaaga aaaatttggt gtgcttttaa aaacacaaga atcagattta gatataaatc
     4621 aattagaaat tgtgttagat gggaaaagtt taattagtgc taatatgaga gacattcata
     4681 gaaaattgta tgacatttat gacctttagt tttaataatg aagggagaga aaagatttgt
     4741 atttaaaaag actagagtta aagggattta aatcctttcc agtaaaaaca gatattattt
     4801 ttaaagaagg aataacagcc atagtaggac caaatgggag tggaaaaagt aatatatctg
     4861 atgctgtaag atgggtatta ggagaacaga gtattaagag tcttagaggt gataaactcg
     4921 aagatgtaat atttgcaggt acagatacaa aaaaacctat gaattattgt gaagtggctc
     4981 ttactattga taattctgaa aatcaattgg aattagattt tacagaagta actataagaa
     5041 gaagggctta tagaaatggt gaaagtgaat tttttctaaa taataaaagt tgtagattaa
     5101 aagatataaa agaagtcttt ttagatactg gtattggaaa agatggttat tcaatcatag
     5161 aacaaggaaa agtagatgaa atattaagta ataatccttt aagcagaaga aaagtttttg
     5221 atgaagcttg tggcatatca aaatatagat ataaaaaaca ggaagcagaa aggaatttaa
     5281 gtaatacaaa ggaaaattta gaaagaatag atgatgtata tatagaaatt gaaaatcaat
     5341 taaagcctct ttttaatcaa cagacaaagg caaaaaagta cttggaaata agtgaaaaat
     5401 taaagacact tgaagtaaat agctttataa gagaaattga aggaatagaa aaagaattaa
     5461 gtgaggttaa tgaacatcgt aaggttattg aaaaggagct caatgaaaaa gaagaacaaa
     5521 aaaatgttgt agagaaaaag caagaagata ttaataaaga ggtagaggta ttacaagatg
     5581 taattgaaaa atctgtagat tatataaatt caattaaagg agttatatca aaaaaagagt
     5641 cccaaataaa tttaattaag gaaagaataa gaaattttac gaatgaaatt agtagaaaaa
     5701 atttagaaat aaaggatata aaagagaagc taaatgaaaa taagcaatac ataaaagaat
     5761 tagaaagcaa taaattatca ggtagtgaag aactatctac cttgcaggaa aatataaaag
     5821 tattggaagg aagtaaagat aaacaaaaaa ttaagttaga atccctaaat aatgagatag
     5881 aattattgaa agaaagtata attgatatat taaataagaa acaagagttt agtaataagc
     5941 tgtctacttt aaacgcaaat aaagaaaata tgaatatcag agatgaaaat ataaattcag
     6001 agataacaga gttaaacaaa aatattgaaa taaaatcatc tgaattagat actataaata
     6061 aagaatttaa tatgcaaaat gaaaatttaa agaatgtaaa taatagacat aaagaattat
     6121 caattaattt gcaggattca attagtgaac acaataaact agaagatgaa atacagaaaa
     6181 gtaaatataa tttaaatgga tataattcaa agttaaatgt ctatatagat atggaaaatc
     6241 attatgaagg atttaataga ggagtaaaag aagtactaaa aaataaaaat ttaaaaggag
     6301 ttcatggagc attaggacag attataaatg tacctgaaaa gtatgagaaa tctatagaag
     6361 cagctcttgg tgcttatatg caaaatataa taactgataa tgagtttagt gctaagtttg
     6421 caataaacta tctaaaacag aataatttag gtagagtaac atttcttcca ttaaatataa
     6481 ttaaatcaaa taaaataagt ctaggaaatt taaaagcaaa tactaagttc ataggaatag
     6541 caagtgattt aattacattt gatgaaaagt atagaaatat aattgaaaat atacttggaa
     6601 gaacaatact tataaataat atagatgagg gcataaaatt tgcaaaagaa actggtcata
     6661 ggtttaaaat agtaacttta gatggtgaaa tcttaaatcc aggaggttct ttgactgggg
     6721 gaagtctaaa aactaatggg aatatactat cgagaaaaag atacattaat gaatatacag
     6781 aaaagataag taatatcaag aatgaaattt cacatctaga attaaaaaga gaatctttag
     6841 acaaagatgt gaaaaatatc aaaaatgaaa ttgattcaca tgaaagtaaa ataaaagatt
     6901 tagaaaaaag tataataata aaaagtacaa gtataaagaa tgttgagtct gaaattgaat
     6961 cgttaaaagg tagtattact aaattagaaa atgagaagaa tgatttaaat tctaacctaa
     7021 attatacact tgaaaaaagt gatgatgtta gaaaagatat ggaagagtta gatgatttat
     7081 acaataaaaa taaagaaaaa attgatgcat tgaatgagga aataaaaaga tacaatgatt
     7141 tatatgataa agaaaaatct gaatttgatg aattgaactt aagtttagtg aagaaaacag
     7201 aagtttacaa tagtatagtt agagatataa aaagaatatc tggtgaaaat tgtgagctag
     7261 aagaaaaaaa taaacaatta gaagagtcat taaattatga agaacatgaa ataattaaat
     7321 tacaagattc tatactcact gaagaaaaag aaaaagaaaa tttaacaaaa caactagggg
     7381 atagtaatag aaatcttgaa actagaaaaa tagcaaaaga tgatttaaaa aatagttttg
     7441 atgaaattaa taaagaatta aaaactatag atagacagca tatagaactt aaagaaagtt
     7501 tatttaaagt tggtggaaga ttagaaagat taaaaactag tcaagacaca tatataaata
     7561 agttgtttga gcaatatgat atgaccttag tacaagcatt ggaaattaaa gatgaagatt
     7621 tggatataga tagaaaattc ttagaaagtc taaagagaga aataagaagt cttggaaata
     7681 taaatataga ctctataaag gaatatgagg aaataaaaga aagatacgat ttttatagcg
     7741 aacaaaagca agatttagaa gagtctatgg aagagataga aaaacttata catactttag
     7801 aagaaaatat gaaatctgaa tttgaaatta agtttgaaga aataagtaaa aattttaaat
     7861 atgtatataa gagattattt ggtggaggtt gtggagaatt aactatttta gataaagaaa
     7921 atttattaga aagtgatata ctaataacag ctcaaccccc aggtaaaaaa atgaaaaatt
     7981 taaacctatt atctggtgga gagaaagctt taacggcaat aagtatatta tttgctatat
     8041 tgattacaaa gccaacacca ttttgtatat tagatgagat tgaagcgcca cttgatgacg
     8101 caaatatatt tagatttgga gaatttttga aggatttatc taaagagact caatttatat
     8161 cagtaactca taggagagga acaatggaag ctgctgatta tatctacgga gttacaatgc
     8221 aagaaaaagc catttcaaaa gttattagtt taaaattaaa agaagctcag gaaataacag
     8281 atattatata acttagattg acataggagg aataacagtg ttaaaaaaat tatttggttt
     8341 tggaaaagac aaggaaaagg aaatagaaaa aaaagatgct gaagaagaaa tagaagttga
     8401 agacagtgtt gataatttag aaaacttgga agaaactatt ttttcaggac ttgaagaaga
     8461 agtgatagat aaagtggaag atattgaaga aaaaaatgag gaaagtgtca ataatgaagg
     8521 cactgaagaa atcgaaaact ttgaaaaaat agaagttgag atggatagta atggaaaaga
     8581 agtagaaaat atttcaaata atgattctca agaagagata atcgaagaat tagaaaatta
     8641 tgatgaaatt gagtctaaag agatagaaga taaagaagat aagaaagtta acttgtttga
     8701 aagactaaaa caaggattga caaaagcaaa gcaaggaata actgatagaa tagatgaagt
     8761 tttaaaatca tatacaaaaa tagatgaaga attacttgaa gatttagagg aaattttaat
     8821 aacagcagat gttggtgtaa atacaactat ggatataatt gaaagactaa gagataaaat
     8881 aaagcaaaaa ggtattactg aaccaataaa ggtaagagaa gaacttaaat ctatagttga
     8941 agatattctt acaaatgaaa actctacact agatatagaa ccagcaccat gtataatatt
     9001 aatggtgggt gtaaatggcg tcggtaagac tactacaata ggtaaactag caaatagata
     9061 caaaaaagat ggtaaaaaag ttttattagc agcagcagat acatttagag cagcagcgac
     9121 agagcaatta gaaatttggg caaatagaac caatgtagac ataataaaac atcaagaggg
     9181 agcagaccct ggagctgtag tatttgatgc tataaaagct gcaaaagcta gaaaaacaga
     9241 tgtattaata tgtgatacag caggaagatt acacaataag gctaatctta tgaatgaact
     9301 tggaaaagta tttaaaatag tagatagaga atttccagaa gcaaaacgtg aagtacttct
     9361 tgtggtggat gctacaacag gtcaaaatgc agttgttcaa gcaaagacat ttaaagaagt
     9421 tgcagatata acaggtatag tacttacaaa acttgatgga actgcaaaag gtggagtagt
     9481 ccttgcagta aaatcagaag tagatgtacc tgtaaaactt attggagttg gagaaagtgt
     9541 agaagaccta caagatttca atgcaaaatc attttcagat gcattatttg gtaactaatg
     9601 ttcttttaaa aataatggtt gacaagagtg ttgttatgat ataaaataca atagtgtaaa
     9661 gtaaataacc tttacagtat actgctagga agtgtaagat atgaatatag aaaaaatggt
     9721 cgaaattgga ttgttatttg aacagtataa agaattatta actgacaagc agaaagaaat
     9781 agttgctcta tactatgaag aagattattc tttaggtgaa attagtgaaa atctgaatgt
     9841 atcaagacaa ggtgtttatg ataccttaaa gcgttcagaa aaaatactaa gagattatga
     9901 agaaaagtta cacttagtat caaagattca agagcaggaa aaaaatatta agattataaa
     9961 agataaaatt attgacatta aagaagattt attgcacaat agagattgtg ctaatttaat
    10021 ccctaagcta gaaaatatag aagatgtatg tagggagatg ataaaatgat atttgaagga
    10081 ttgtctgata aactacaagg ggcatttagt aagttaaaat ctaaaggaaa acttacagaa
    10141 gtagatgtaa aaaatgctat gagagaagtt aaacttgctc ttcttgaggc agatgttaac
    10201 tttaaagtag ttaaagattt cataaaaaaa gttcaagaga gatgtgttgg gcaagaagtt
    10261 atgcaaagct taactccagc tcagcatgta atcaaaattg taaatgaaga gcttacaagt
    10321 ttaatgggag atgtacaaag taaagtaatg atttcctcaa agcctcctac aattttaatg
    10381 atggttggat tgcaaggtgc aggtaagact actacagcag gaaaacttgg aggatatttt
    10441 aaaaagcaag gtaaaaaacc tctattagta gcttgtgata tttatagacc agcagctata
    10501 aagcaacttc aagttgttgg agaaaaattg gatatacctg tattcaatat gggagataaa
    10561 gaaaatccaa taaatattgc gaaagcaggt ttaagccatg cgattaaaaa tgctaatgat
    10621 ttagttatta tagatacagc tggtagactt catatagatg aagtacttat ggatgagtta
    10681 aagtctatta aatcagaagt taagccacat gagatacttt tggttgtaga ttctatgaca
    10741 ggtcaagatg ctgtaaatgt tgcagaaagc ttcaatgagg cacttggtgt tgatggagta
    10801 gtattaacta aattggatgg agatactaga ggtggtgctg cactttcaat aagagcagtt
    10861 acacaaaagc ctataaaatt tatggggatg ggagaaaagc tagatgatat agaacctttc
    10921 cacccagata gaatggcatc aagaatatta ggtatgggag atgtcttaag tttaatagaa
    10981 aaagcacaag aaaatataga cttagagaaa gctaaagaat tagagcaaaa aattaaaaaa
    11041 caagagcttg attttgaaga tttcttacaa caaatggaac aaatacaaaa tatgggacct
    11101 cttgacaaga tacttggtat gatacctggt atgggaaatg taaaagacca acttggagat
    11161 gtagatttga atggcaaaga aatgaagaga acaaaagcca taattcagtc aatgactaca
    11221 tatgagagaa gaaacccagg agtattaaat gcttcaagaa agaaaagaat agctaaaggc
    11281 agtggaacta gtgtacaaga tgtaaataga cttataaaac aacttaatga aatgaaaaaa
    11341 atgatgaaga tgtttgctgg ttcacaaaag agtatgaaga aaaggggcgg cttaggcggt
    11401 ttaccttttt ttaaataaaa tataaaaaag taaaaattat tcggaggtga ctgtaatggc
    11461 agttaaaata agattaaaaa gaatgggagc taacaaaaaa cctttctaca gaatagtagt
    11521 agctgattca agagctccaa gagatggaaa atttatagaa gaaataggat actataatcc
    11581 aatttctgaa cctaaacaag taagaataaa tgatgaaaaa gctataaaat ggttagctac
    11641 tggtgctcag ccaacagaag tagttaaaaa attacttgta aaaaacggag taatagaaaa
    11701 attcgaagct tctaaacaag caaaataatt ttttcttggc aattagttta agtaagggct
    11761 cattgctaag gttactttag gagatgaaat atatgaaaga gctagttgta gatatagcta
    11821 aggctctagt tgataatcct gactcagttg ttgttgagga atttgaagat aatgatggta
    11881 tcgtcttaaa gcttacagtt gctcaagagg atatgggtaa ggttattggg aagcagggaa
    11941 gaatagctaa agctataaga actgtcgtaa gatctgttgc aaatagagaa aatataaaag
    12001 tttctcttga gatagtataa aataggagga ttaggtataa tgcctagtcc tttttatata
    12061 aatgcgtagt ttaagatttt atgatgatat gaccacataa gattaaattt tttaagaagg
    12121 tgataacatg aaagaaaaat taactcattt taaggtaggt caaatagtaa atacacaagg
    12181 tttaaaaggt gaagtaagag tttatccgct tactgatgat atagagaggt ttgatgaatt
    12241 aaaagatttc tatctgggaa aagatttaaa taataaattg gctgttgaaa aagtaagata
    12301 caaaggttct atggttataa tgaagataaa aggcatagac agcatagaga aagcagaaaa
    12361 actaaaaaat aaatttatgt atgtagctag agaagatagc agagagcttg aagaaggcga
    12421 attttttata gcagatatga ttggaatgga agttttaact gtagatggaa aatatgtagg
    12481 aatactagaa gatgttcttc aatactcagc aaatgatgtc tatgtagtaa aaggtgagga
    12541 agataaagaa tttatgatac cagctataaa aaaatttgta cctacaatag atattgatga
    12601 aagaaaaatg attatagacc ctattaaggg aatgatagat taggtggtaa tatgagattt
    12661 catataatga cactttttcc agaaatattt aattcgtaca tggatgaaag tataatgaag
    12721 agagcagttg aaaaaggtat aattgaggtt catatatata atataaggga tttttctaat
    12781 aataaacata aaaaagtaga tgattatcca tttggaggag gagcggggat ggtaatgact
    12841 cctcaaccta tatatgacac atataagcat ataataacaa ctcataatat aaataaccca
    12901 agtgtaattt atcttactcc aaaaggtaag gtatacaatc aatctatggc aaagcagatg
    12961 tctttaaaag aagatataat actactatgt ggtcattatg aaggaataga tgagagaatc
    13021 attgatttaa tagttactga tgaaatttca attggagatt atgtattaac tgggggagag
    13081 ctcccagcac ttataatgat agattctata tcgagattaa taccaggtgt attaaaccaa
    13141 gaggaatcat ttgaagaaga atcatttaaa gataacttat tggaatatcc tcattacact
    13201 agacctagag attttgaagg attaaaagta ccagaagtgc ttttatcagg gaatcataag
    13261 aagatagatg agtggagaag agaagaatct ataaggataa ctaaagaaag acgatttgat
    13321 ttatataaaa agtctaatga aaaataaaaa tataatgaaa aattaatata tatgtataca
    13381 ataaagtacc tataaatgga attaaaaaca taaaagtgct tgtaataact attgaagtat
    13441 agtataatat aaaatgttga aaatacgggc gttcctctgt ttacactggt cgggtagtta
    13501 agaacgtcta tgatatctag gaggaagaaa gatgaatgaa atgctaagag caattgagca
    13561 agaacaatta aaaaatgaag ttcctaattt tggacctggg gacacagtta aagtacacgt
    13621 tagaattata gaaggaaaaa gagaaagaat acaagtattc gaaggtgtag tattaaagag
    13681 acaaggtgga ggagcaagag aaactttcac agttagaaaa atgtctttca atgttggtgt
    13741 agaaagaact ttcccagttc actctccaaa aatagaaaaa atagaagtta ctagaaaagg
    13801 taaagtaaga agagctaaac taaactactt aagaggtaga gtaggtaaag ctgctaagat
    13861 aaaagaagct agaaataaat aattaaaaac tttacttata aatggggggc tgatttatca
    13921 gtcctctttc tattttaatg aaaattacag caaataatta tactagaaat aaatagaaat
    13981 atttgttgaa aatatacatt atttatctat gaatttatag cttttaaaat gaaaaataaa
    14041 attggtaaat attgcattat attttgtaac aatattaaaa ataagagaac agtattatgt
    14101 aaagtatgac gttaaaaaga tgacaatatt atagtttgtt aagatagatt ttttaagtgg
    14161 aattaggata taataatata atattaataa ggagtgatac atgtgtcaaa tgaatacgaa
    14221 gagtatctta tgaatgacaa tctacatata aactggtatc caggtcatat gaaaaaaact
    14281 aaggagttag taaaaaataa cttaaaattg attgatgtag taattgaact tttggatgct
    14341 agaatcccgt ttagtagtaa aaatccagac atagacaggc ttgtaggaga taagccaaga
    14401 gttgtcgtac ttaataagag tgatatggca gatagggata aattgaatca atggattgaa
    14461 tactataaga aaattaatat caaagctata ccagtagata caataaaagg cgttggtata
    14521 aataagataa tcgaagaatg taaaaatgta actagagaaa aaatgagttc tttaaaagac
    14581 aaagggagaa aagaaagagc tatacgaata atgatagttg gagttccaaa cgttggtaaa
    14641 tcgtctctta taaacaaatt aacaggtaga aaaagcactc aaactggaga taagccagga
    14701 gtaacgaaag ggaaacaatg ggttagatta aagggaaatc ttgaactttt agatacacct
    14761 ggaatacttt ggcctaagtt tgaagaccaa gaagttgcat taaatttagc atttagtaga
    14821 gctataaagg atgagatttt agatactgaa actttggctt taagacttat agagaaactt
    14881 atgaaaatag aacctgagaa gttgaaagcc agatataagt tagattgttt aggagaaact
    14941 ccaattgaaa ctatggatat gattggccat aaaagagggt ttataacagg taaaaaagaa
    15001 ctagattata cacgcatagc cacaactgta ttaaatgaat ttagagatgg aaaaatagga
    15061 aatataacat tggaagttcc agaaaacgtt aaaagataga ttttaaaaca aaatcaaaaa
    15121 tttaatgtta aaaatttaaa aaagcctttt agcaaaggct tttttatttt tttgaaatat
    15181 agacaaatat ctccatataa aagtataaat taggttagaa aacctttaaa aatattaatt
    15241 taagacaaat atacctaata ttttgaaaat taaaatgagt gtgattattt aaaattttag
    15301 catattgact tgcttgtttc gtataatata taataagcac aaataaatac aatatattaa
    15361 aaatttaatt tatttaattt tattaattta ataataagtt cttattgttt aattcaactt
    15421 acttatgcaa gcgtaaacaa tcgagacatt ttattaattt aataaggtta aaaaggggga
    15481 ttttataatg ggtaagacca aggatgttat agtatttgga tttgcgttat ttgcaatgtt
    15541 ctttggagca ggtaacttaa tatttccacc ttacttggga ataattacag gtccagaatg
    15601 gttaattgcc ttcttaggat ttacgtttgc tgatgctgga ttggcacttc tagcagtaat
    15661 ggctacagct aaattcgatg gaaacgtagt tgaaatgttt aaaagatgtg gtatgaagtt
    15721 agggattctt ataggttgtg cagatatttt atgtataggc ccattcttag ctataccaag
    15781 aacaggtgca acaacttatg aaatgggtat aatgcctttg tttggaactt caattccagt
    15841 gttattattc tgtatacttt tttttgcgat ttcatatgta ctaacaataa gaccttctaa
    15901 ggtcgtagat atagttggac agtttttaac tccagctctt ctaatagctt tagcatttat
    15961 aattataaaa ggtattatat cacctcttgg agatatagta gataaaccta tgatacctaa
    16021 tgtatttgca gaaggtatag gtcaaggata tcagacaatg gatgcttttg cagcaatagc
    16081 acttgcatca gtgctaatag tatctttaaa tgataaagga tattctacta ttagtgacaa
    16141 gttgaaaatg attggtaagg cgggtgttct tgcttgtgga ggattagctc tagtttatgg
    16201 aggattatgt ttccttgggg caactgtatc aacaatgtat ggaacagatg cagtacaatc
    16261 acaagtaata gtaaatataa ctgaaggatt gcttggaaat gttgggaaag cgattttagc
    16321 agtagtagta tcattagcat gtttaactac atctataggt cttacatctg ctacaggtca
    16381 atatttctct agacttacaa aaggtaaatt gagttatgaa aaaatagtat tagctgtatc
    16441 tgtattcagt gcagtagtag catcttttgg tgttggaact ataataaaga tagcatctcc
    16501 tatactaagt atagtttatc caccatcaat tgttttaata attttagctt tcttcaatga
    16561 aaaaattaag aatgataatg tatacaaagg agcagtatat atgtcattgc ttgtaagtat
    16621 actaacagtt atatctagtt atggagtagc tgttccagta gtaaattctt tacctcttaa
    16681 ttcattagga tttaactggg tggtgccagt tataatagct ggaataattg gtaattttat
    16741 accatcaaag agtcaatcta atacacttgg aacaaactaa gttttgttta attaaaatat
    16801 aatcaattgt aaataaatga gtt
//
LOCUS       DAGSHT010000061        16674 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGSHT010000061 DAGSHT010000000
VERSION     DAGSHT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.063"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            45..350
                     /locus_tag="MJB72_001848"
     CDS             45..350
                     /locus_tag="MJB72_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262958.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            758..1447
                     /locus_tag="MJB72_001849"
     CDS             758..1447
                     /locus_tag="MJB72_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8262959.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1447..2364
                     /locus_tag="MJB72_001850"
     CDS             1447..2364
                     /locus_tag="MJB72_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8262960.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2437..3363
                     /locus_tag="MJB72_001851"
     CDS             2437..3363
                     /locus_tag="MJB72_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262961.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            3360..4040
                     /locus_tag="MJB72_001852"
     CDS             3360..4040
                     /locus_tag="MJB72_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8262962.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4138..4473
                     /locus_tag="MJB72_001853"
                     /pseudo
     CDS             4138..4473
                     /locus_tag="MJB72_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            5457..6107
                     /locus_tag="MJB72_001854"
     CDS             5457..6107
                     /locus_tag="MJB72_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262963.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            6459..8525
                     /locus_tag="MJB72_001855"
     CDS             6459..8525
                     /locus_tag="MJB72_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBY8262964.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVVDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            8542..10497
                     /locus_tag="MJB72_001856"
     CDS             8542..10497
                     /locus_tag="MJB72_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBY8262965.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            10513..11703
                     /locus_tag="MJB72_001857"
                     /pseudo
     CDS             10513..11703
                     /locus_tag="MJB72_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            12852..13226
                     /locus_tag="MJB72_001858"
     CDS             12852..13226
                     /locus_tag="MJB72_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262966.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            13228..14085
                     /locus_tag="MJB72_001859"
     CDS             13228..14085
                     /locus_tag="MJB72_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262967.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            14088..14726
                     /locus_tag="MJB72_001860"
     CDS             14088..14726
                     /locus_tag="MJB72_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY8262968.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            15732..16466
                     /locus_tag="MJB72_001861"
     CDS             15732..16466
                     /locus_tag="MJB72_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8262969.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIETLKNLIIE"
BASE COUNT         6267 a         1849 c         2828 g         5730 t
ORIGIN      
        1 atatttttat agtagtagtt aattattaat aaggaggggt caaaatgcct agcatgatta
       61 tggaagttgt aggagtacta tttatagcta ttggattaat cttgacattc attgaaataa
      121 aaggaaattt cccttctttt tatggttttg gtcaaaattc tatagggaaa aatgctgtag
      181 gtattatagc agcaggaatt gctttaattt tctttagaaa tggcatagat agcatagatt
      241 ctatagctac ttatggtgta tttttagcaa tagaacttgt tgtaatagtt gcatatttct
      301 taataagaaa aaaatttact aaagaagata aacatttaag aactaattaa ctaaattatc
      361 tactataaaa ttggaaaatt aaaaacaaat aaaagtttgt ttttaattat ttaaaatcca
      421 aatttgatta tttttaaaga aactatgttc tgtataaaac tagaatgtgg tttctttttt
      481 atgtataaaa tattttttag caagagttaa aaggtgtttt ataatataaa atagttaatt
      541 gtagatgtta ataaaagaaa attatgtata tttcagataa agataagaga gtatactata
      601 ttttaattat tttttcaata aagcttattg aaaagaactt taacttagaa gagtatcacc
      661 aaattgcaga tacaatatca taagcagtta tttattgaca acccttttta ataaatatat
      721 catttgaagt atactgacga aaaggagttg aataataatg gacatcttat tggttgaaga
      781 tgataagaat attcgagaaa tggtatacga ttttctgatt agtgagaatt actctgttaa
      841 aactgccatt gatggaaata cagctattga aatatttaaa aattattcat ttgatattat
      901 tttactggac ttgatgttac caaagtctag tggttttgaa gtattaaaat atatacgaag
      961 tgtgagtgtt gttcctgtta ttattactac tgcgaaagat agtgacacag ataaaatgct
     1021 agggcttaat cttggtgcag atgattatat tacaaagcct ttttcaatcg tagaattgct
     1081 tgcaagaatt aaagctaata ttagacgtgc aacagtgtac agcagttcaa gaaactctga
     1141 agatatggtt attcagtata aagatttgat tataaatata tcaaaatatg tagtttcaaa
     1201 gaatggaatt gacttaaact taacacatac agaatttgaa attgttaaat tactagcgac
     1261 taatcaagga cgtgcttttt caaaggaaca attatacaac gctatttgga aagaacctta
     1321 ttatggaaat gaaaatgtgc taaatactca tattaacagg ttaagaaata aattgtctga
     1381 tggtatagaa aaaaataatt atattaaaac tctttgggga attggctaca aaatggagga
     1441 agaataatgg agttattttt agttgtaatt tcgattgtac tatttgctct ctacttaaaa
     1501 gaacgaaata aatcaaagaa aatggaaagt gattataagt atatcaattc aagacttcgt
     1561 gacataacaa atgatgatga aattaattat attttagtac catctgatat ttcgattgta
     1621 aaagaaacag cagtagatat aaataaactt cttgaaaagt tttacagcag gcaaattaat
     1681 tataatagaa gtgaaaaagc gatgttgcaa atatttacaa acatatcaca cgatttacga
     1741 actcccatca cagtattaaa gggttatatt gaaatgttat atcttcaaag tcaaaaagaa
     1801 gaattatctt cttcaatgag agcaactgtg gaaaaaatgc aaacaaattc aaatgagctt
     1861 gttaattcaa taaataacct gtttaatatg gcaaaaatcc aatctggaga tatgatatta
     1921 aatcttcaaa aatccaatct aacacaatta tgtcatgaga ttattttaga gttttatgat
     1981 ttgctagata aagaaaactt taatgttgaa gttaatattg aagatagacc tatatatgca
     2041 aatgttgaca cagaagcatt acaacgtata ctcaaaaatt taattgataa tgctataaaa
     2101 tatggttcag atggtagatt tttagggatt tcactatatg aaaaggaaaa acatgttttt
     2161 atagatattg aggataaagg ttcaggtatt tctgaaactg aaaaagagca tattttcaca
     2221 agagcttata ctgctgacag aaaaaaaggc aatggtttag gtttggctat ttctcaaggg
     2281 cttgtaaact ctatgggagg ttcaatttat gtaaatagta gacctaatgt aaaaacagtt
     2341 tttacagtaa aattgaaaag ttagaaaaaa gttagattta aaagagagaa aagataattt
     2401 tattttgtat actgttttta gaaaggaggg agtatgatga actatatcat agagaccaat
     2461 caattaaaga aaatatataa agataaagct gttgttaatg ctgttaatat tcatgtgaaa
     2521 aagggagaaa tttatggatt tgttggacca aacggggcag gtaaaagtac agtaatgaaa
     2581 atgttgctaa atcttgttaa gccaaattct ggagaaatag ttatgttcgg taagaaagtg
     2641 gcagaaactg attttgaaat attaaaaaag attggtacta ttattgaaaa tccgtattta
     2701 tatgagaatt tgactgctaa acagaatctt gatttacact gtgaatatat gggttattat
     2761 aacaaagaac atatatcaga ggtattggaa tgtgtcggat tgtcaaagca atcaaacaag
     2821 aaagtttcaa aatattcttt agggatgaaa caacgtcttg caatagctag agcaattctt
     2881 actaaaccag aattattgat tcttgatgaa cctataaatg cactcgaccc agaggggata
     2941 agagaaatgc gagaattatt ccgtaagtta aatacagatt atggaataac tatatttatt
     3001 tctagtcata ttctttcaga ggtagaacaa attgcggaca ctattggaat tattcaagat
     3061 ggtaaactta ttaaagaaat ttctatgtca gatatacaca aatatcaaac agattatgtt
     3121 gaagtagatg tagataatgt ggagcttgct ggatacttgt tagaaaaaga atttggaata
     3181 aaagacttta aaattacttc ggaaagttgt attgaaatat atgatttacg aaaagatgta
     3241 aaagaaatat ccaaaatatt tattcaaaat caaattggta taaatagcat tggacgtaaa
     3301 caaagttctt tagaagatta cttcttccaa actacaggta caggaggtaa agaaacacta
     3361 tgatacattt aatgaaacta gagctaaaaa aagttgggct aaaaagatat tttttatttt
     3421 caatgctggg gattttctta tgtatgtttt ttgtctttac tggattgaat gataaatcta
     3481 cttcaatata tgattatgat gttacattta ggacaatcag tttatttttc tgtttttact
     3541 atattatatt atttgctgtg ttgaatgtgg catatataat aaatgaatat acaaataaaa
     3601 caatcttgat tttattctct tatccacttg accgtaaaaa acttatttta tcaaagttac
     3661 ttttgattac gactcttatg atatcatcaa tgatagttgg atatatctgt tgttttacat
     3721 tcgttgttgt gttggataag tattttaatt tagttattgg agattttcat atttcaattt
     3781 tatcttattg gataccagga gcaattaaaa gtattattgt attttgctca ttaggtatat
     3841 ggacattcgt tgttggaatg ataagaaaat caatacctgt tacccttgtc agttcaatta
     3901 tttttatcaa tttaagacaa tttattttag ctggtacaaa tacaactcaa gacagtttga
     3961 tttctgtttt tattatagtt ggtataactg tgattggtat atattatata ctaaatcata
     4021 aagttactga aattgactag atactgtgga taggaaggta gttattaatt gtatcttctc
     4081 gggataataa tgatttgcta ttatatcagc tattattaat attattagag ttttaataat
     4141 attgcacaac catttgaagt tgagccaaag cttgatgaat taattgaatg gtactatagt
     4201 cagagtgaag tatctattaa agttataact gaatttcatt atagatttga attgatacat
     4261 ccatttcaag atggtaatgg tcacataggt agatttgtta tgctaaaaca gatgttagaa
     4321 aataatcttc ctataaaaat tgtatcttgg gatagtgagg aattgtatag aaactcctta
     4381 aattcttgta gtttaggtaa ttatgtccct ttaattgaat atttatcaag tttagaagat
     4441 tttagagaag tatataaaat gttatggaaa taattgtttg cagcaaaaaa agtttatata
     4501 taaatgtatt tttatctatg ctatttaacg ctaatttctt agttattctt agcttggact
     4561 atacccacta ttttaactat ataaaatagt ggatatagtt taactatctc tttataacta
     4621 tttttatgta tcttagttgt ttcttaatat ggggacagtt cataaaacta aaagtgattt
     4681 ttaattttat catttataat ttaccattac atcagaaaaa tgcttatcat tagaattttc
     4741 ataaaaggta cctatttttt tcaattaact gaatcaattc tattagtctt aatctttcat
     4801 atttcaaatt gataatataa gaaaataaaa aataaaaaat tttaaatttt ttatttattg
     4861 cgaaaaaaat aaaaaggtgc tatacttttt gtaacatatt attcaaatta ttaagagata
     4921 gtaaacattt atagtttaat ttgtagtaca ctagcatgat ttgcagaaca atgaattaat
     4981 ctatagtata atagtttaat ttaaagtaca acgatttaat ctatagtaca ataacttgat
     5041 ttgaagtaca acgatttaat ctatagtaca ataacttgat ttgcaataca atgaattaat
     5101 ctatggtata atagtttaat ttgaagtaca atgaattaat ctatagtata atagtttaat
     5161 ttgaagtaca acgatttaat ctatagtata atagtttaat ttgaagtaca atggtttaat
     5221 ctatagtata atagtttaat ttgaagtata acgatttaat ctatagtaca ataacttgat
     5281 ttgcaataca atgaattaat ctatagtata agagcatgat ttacagtaca ataatttaaa
     5341 ttgtagtaca aaagtactat caaataagaa tttctacttt aaaaatatta ctaaaagtaa
     5401 ttaaagtcca gtaattcaat ataatatggc aaaagaatag aaataaggtg agagtagtga
     5461 aagtaaagtc aaatttagat gaaattttat atcataagat tattgaaagt cttattcgtg
     5521 gtgaatactc tgtaggtcaa aaaatattac taaatgacct ttgtgaaaaa tttgaagtaa
     5581 gcagaacacc agttgtacaa gcagtaaaaa tgctaaataa agatggagtg cttactatta
     5641 tgacaaatgg taaagtgtac gtaccagagt atgaatatga tatggtaaaa caagtttgtg
     5701 aaacgagaac actgattgaa acatatgctt tagaaaaaat gatgcaagaa gaggaagagg
     5761 tttttcaaca aaagctagat gtaataaaaa gatactctga taaatgcgag gagtactatc
     5821 gtcaagaaaa atctgtagaa cttgcactag cagatttgga attacataaa gccattgtag
     5881 aaggggcaaa taatgagatt ttaaatgatg tatatgttgg tatacaagga agatttatag
     5941 tagtgaacta tttaattcga ccattaaaaa atcgtaatta tgaaggaaca gtacaagacc
     6001 attttgaaat attaaaattc attgaagaca gagatacaaa aaaagcagta gataaactaa
     6061 gaaatcatat tcaaggaact attaaaagat tctgtgaaga tgaataataa gttttgaagt
     6121 tactatatat acttatctat taagttgcta aatgtagtct aagtataatc taattatagt
     6181 ttaaacataa tctaaatata atctaataaa tataatctaa taaatataat ctaaatatag
     6241 tgtaaataag tctaagtatg aattaaatat agattaagta tggactaagt atagattaag
     6301 tatagattaa gtatgaatta agcatgaatt aagcatggat taaatatact ttaaattaca
     6361 aatattacaa ggaagccaga aggcttccta aaaaaataac ctttataaaa ttttttattt
     6421 taaatttaaa attttttatt ttaaatttaa aataaaaaat gaaagaggtg gtagggagtt
     6481 tgaaaaaaat tactaaagaa aggatagaaa atactatgaa aaagctattt acgagtgaaa
     6541 catttttgga atatcaattt ataagtgatt gccaaatatc accagatgga ggatatacag
     6601 catttattgt aaaaaaagct gatatcaaag agaatggtta cacaagccaa gtttatatat
     6661 taaacaataa aactggcgaa ttaaaacaaa taacgtctat aaactctgtt ggagcatatg
     6721 cctgggaaga tgaaaatact atcttgttcc ctgctttgag aaatgaaaaa gtaaaagaag
     6781 ctgtcaaaaa cggtaagcaa tgcatgagtt actatgcttt atctttaaat ggtggagaag
     6841 cagaagaact ttttagactc cctatcaaag gtggtaaatt gaatcctata ggaaaaggtt
     6901 tgtatgcagt tgttgattct tatgacaatg accgacctat tgtagaggga ttgccagaag
     6961 aagaacaaca gaaaaaaata aatgcttata acaaaagaca ttatgaacat ttcaaagaaa
     7021 ttccttatgc tgtcaatggg gagggatata ttagcagaaa aagaaaacgc ctatatgtat
     7081 acgacagtaa tataaatgag ttgaaagcaa ttacagcacc tatgtttaat gttgtaggta
     7141 tgaaaataag tgatggaaaa atcttatata tagggcaaga gtttaaagat gtaaaaggtt
     7201 tgaaaaatgg ggtctatgta tttgatacaa acacaaacac aaatgtctgt atattagaaa
     7261 aagataagta tattataaaa ggttttgaat tatatcaaaa tcaagtgata ctaaacctaa
     7321 ctgatgcttt atcttatgga aatggagaaa atggagattt ttatactatt gatattgata
     7381 cgaaagaaat gaaacttctt tcagggcatc aacatcattg tattggaaat acagtaacat
     7441 ctgatgttaa aatgggtgct ggtcagacaa caaaggtaga tggagaatat atttattaca
     7501 cttctacagt agatatggac tgcattattg aaagaataca tattccaaca ggaaaacagg
     7561 aaaaagtaac acagacaggt tcagttgatt ttatagatgt aaaagatggc aatattgtat
     7621 gtgtgggttg tttaggaaat ggattaccag aagtttatac agtagagaat ggaacattgt
     7681 gtaagaaaac acatttaaat gaccatattt tagaggaata taaaatttct gtgccagaat
     7741 acatagagtc aaaaggaagt tctaaatggg aaatacaagg atacgtatat aaaccagtag
     7801 attatgaggt ggggaaaaaa tatccagctg tattggcgat tcatggagga ccaagattga
     7861 catatggacc atattttatg catgagatac aggtatttac aagtgcaggt tactttgtat
     7921 tcttctgtaa cccaaggggg tctgaaggtc gtggcaatgc ttttgcagat attaggaaac
     7981 agtttggaga tattgattat atagatttta tggaatttac agatactgta cttgaaaaat
     8041 atacagacat tgataaaaca aaattggctg ttgaaggtgg aagttatggt ggatttatga
     8101 cgaattggat aattggccat acaaaccgtt ttgctgttgc ttgtgctcag cgttctattg
     8161 caaactggag tggcatggag ggtacaacag acattggtta ttatttctgc aaaggacaaa
     8221 caggtgcttc tcatatggaa aaccacgagc ttcaatggaa acagtcacct cttgcttatg
     8281 ctgataaatg tgttacaccg actttatttt tacatggtga aaaagactat agatgctata
     8341 tgcaagaagc attccagatg ttttcagcat tgaaaattca tgggtgtcct acaaaacttt
     8401 gtctatttga aggagaaaac catgaactgt ctcgttctgg tagaccaaaa caaaagctac
     8461 aaagattagt tgaaatgcta gattggtttt cggtatatat aaaaaaagat aacataggac
     8521 agtagaaagg agaatataaa aatggaaaaa ataaagattg aaagttcaga tatttttgaa
     8581 tatatattcc ctcatgatat ttcttgttca cctgatggaa agcatattgc ttatattata
     8641 tcaaatatca acgaggaaaa agactgctat gaacatgact tatatgtgat ggacatcaaa
     8701 acagaaaaac aaatacatat gactcaaaca aaagatgtaa catcatttag ttggatttct
     8761 aatacagaac tcctttttac atctaaaaga aataagccaa aatcaggtac aacagatttt
     8821 tacactattt ctatagaagg aggagaagca aaaaaagcat tttctattcc aaaagcttgt
     8881 agcgtacctg tatcacttgg aaataaattg tggttactga caacaaaaaa tccaacggat
     8941 tctaaaaaat cagaaccaga tagggcagta gagggtgtag attattggac atttacagat
     9001 aaacctttta taagagatgg agaaaatttt tctcaaagaa gacgtgtaac attagaatta
     9061 tatcaagaag gtgaaaatat aacaaaagcc attacaccaa aattttgtga agtagcaggt
     9121 gtagacgttt cttctgataa aaacagaatt ttatatactg gtcagattta tgaagattgt
     9181 gcaactcctt tttctggact ttgggagtat catatagaca gtggtgaaac caaagaactt
     9241 gtaccacaag gaaagtacca aataagtttg gcaaaatata ttggcaaaga taaagtaatg
     9301 ttgcaagctt caacattaga ccgttctatc acacagaatc atgatatttt tatacttgat
     9361 ttgagtacta gtgaaataaa tatgattgct agtccagatg gtatgtatgc tacattactg
     9421 gatgtagatg cagtatatgg tggtggacgt agtaataaag ttataggaga taaatttatt
     9481 ggtgcaagaa tttgcagaac tatgacagaa tttaatgagt ttgatacaaa gacaggtaat
     9541 atacgtatca tcacaaaagt agatgctttt acatcttttg atatttacga caacaccatg
     9601 tacacagtaa tgttgaaaga ttatgagtta gcagaaatat atagcattga tatgactaca
     9661 ggtacaatga aaaagatgac tgctttttct aaaccatatt tagatactca taaggtttca
     9721 ttacctgaaa aattgacgtt tgtagcaaaa aataaagaag aagttgatgg ttttgttata
     9781 ccacctatag atgctaaaga aggcgaaaaa tatcctgctg tattgttcat tcatggtgga
     9841 cctaaatggg catatggcta tatgtttaca cacttgaagc agtgtgtaac atcaaaggga
     9901 atgtatgtga tttattgtaa ccctcatggt ggtgacggtt atggtgaaaa attcttagaa
     9961 atggtagaaa gatgggggta tgttgactat gagcatctta tggaatttgt agatacttgt
    10021 attgaaaaat atcctgggat tgatgcagac cgcttagggg ttgcaggggg aagttacggt
    10081 ggttatatga caaactggat aattggtcat acagaccgtt tcaaagcagc agtttctcaa
    10141 agaggtatta gtaacttgat tacagcttct ttaattattg actttgggga tagaattatg
    10201 aagcagactt gtggagataa gacgccttgg aatcatgagg aggttctttg gaatcattca
    10261 cctattaaat atgtgaaaaa tgttaagaca ccaacattgt tcttacatag tgatagggat
    10321 tatagatgtt ttatggggga tactttccaa atgtttacag cattaaaaca acttggtgta
    10381 gatacagaaa tgtatttgtt ccacggagat acacatggat tgtctagaaa tggaagacca
    10441 tctaatagaa ttgcacgagc aaatgctatt gtagattggt ttgaaagata tttataataa
    10501 gggggttatt ctatgagtga tatgttatta tggtttagtg tcgtattagt tttatttgct
    10561 attggggatt taattgcaag caaaacaaaa gcaaaagtat cagctgtatt tgttacacta
    10621 cttttatttt tgattctatt tgtcacaaaa gcaataccag ctgatattat ttaaaaagca
    10681 ggtatgaccg cagcagctag ttggagtgtt ccaatgatta tgtttagtat gggaacaatg
    10741 ttgaatgtta aacaatttat agacgaatgg agaacagtat tgacagcatg gcttggaatt
    10801 gtagcagtta tagtctgtgt ctcattatgt attcctttgt ttggaaaatc aacagtattg
    10861 acaagcatac cagttataaa tggagcttta cctgctacta ctattatgac acaagcagca
    10921 ctagaaaaag gactgacact tgcagcagct acagcaacag ttgtatttgc aattcagaag
    10981 tttattggta caccaatagc ttcaagagca gcgttacaag aggcaaaccg tcttttagta
    11041 gaatatcatg aagcaaaatc aaaaggcatt gatttagcaa atgttgatac tgataagaaa
    11101 gaagcagaga atacaggcac aaaagtaaaa caagctttct gtgaaaaata cgataaatat
    11161 tattctacaa atgtttgtat attttttata gcattattct cttatctagg gtatgaactt
    11221 tcagagatta ttcatgtaaa ttattctatt gtatgtcttg ttgttggtgt tattgtaaca
    11281 cgtattggta ttgtgccaaa agacattttg gaaaaaggaa aaatcaaggg atttatcaac
    11341 atggttgtgt ttgcagctgt tattccttct ttagcaaaag tttctttgac agatttaatt
    11401 agtctttttg taccaattgt gggtatgttt gcagcttcta tcattggtat attccttatg
    11461 atgaaggtgt taccaggttg gaaaattatc ggttctaaac ctcttgcttt tggagtaggg
    11521 ttttgtcaga tgttgggatt cccaactaca tatttaattt caaatgaagt gtgtaatgct
    11581 gttggagaaa cggaagaaga aagagcatat ttgatgtcta agattatgcc taagctggta
    11641 gttggtggta tggcttgtat gatttctatt gtggtagctg gtcttatggt accaatgttg
    11701 tagataggtt tcatagttaa aaaagtggta ttaatttata ggagaagcta aaaagtagta
    11761 ttaatttata aaataatttt atattagtta atttgttata agaatatttt ctttttataa
    11821 ttataatttt gattttataa gatagcagat tttttatttg ctatgctaat agttataatt
    11881 tgtttttatt tgctaaaatg caactttaat ttgatttatg tataatatag ggtatttaat
    11941 aatatttttg aagaattttt ataaaaaatt aaaaagagga gaaaaaaggg agtgccacaa
    12001 agtgaacttt ttagttctaa ggcactcttt tttgtacgaa atcgaatata ttttcaacgt
    12061 tttaaaagtg agaaagagac ttatctctag tttgagatag ccaattttaa ttattaaaat
    12121 tattttttgt tttatttatc aaagagtatt cgtgttttag aagttagtat tttttatttt
    12181 atatatacaa ggttttccgt ttctaatgtt ttctttttta aaataattag ggtattcaat
    12241 tatggtaaaa ggtggatggt attttgcagt gatattattt aaagcacagt attgaatgtt
    12301 gaaatcctca cattttagag tattatcttt tgtaatcaaa aaaatcttct atacttatag
    12361 caaggttatt tctgcaaata ttccatacat aataatatcc caatttaaat ttttttaata
    12421 tagagtttac ctctagtcta cactcttgaa taactctttc ctctatccaa attcttataa
    12481 attcatccca tttattacta tttatttatt actatctaaa ttttttagta tgtttgtaat
    12541 tagaaatgga tttattcctt tatgtttttt ataaatagtt tataacttaa tttatcatca
    12601 aacaaatcat tcatacagga ttactttttc ttttagatat tcaattctta cttttaataa
    12661 atatttaata cattctatct tatgtggttt taaaaagtgt ttatggctat taacaatctc
    12721 taatatttca ttcatattta ttttgtcata gtaatatttt acatctttta tattgacaaa
    12781 aaagtgctct aagtgtatag tgtgtatata acaacatcac actgaagatg ataaaaaggg
    12841 ggtgataaaa aatgaaagtg ctcatttcta acacatcaga taagccatta taccaacaaa
    12901 ttaaagagca aattaaagaa gcaatattta ctgaagaact gaaagaaggg gatgcaatgc
    12961 catccataag aaattttgct aatgatttaa aagttagtat tttgactatt agacgagtgt
    13021 atgaagaact tgaaaatgaa ggctttctta cacgccaagt tggaatagga acatttattt
    13081 caactggaaa tttagaaata ctaagagact caaagagacg tatagttgaa caaaaaatgg
    13141 cagatatgat taaggatgca aaagcactag gaattacaca gatagagtta caagaaatga
    13201 tggatattct atatcaggag gattaatatg gaaaatattt tagaagttta taacttaaat
    13261 aagacttata ccgatttttc attatctaat ataagctttg cattaccaga agattgtata
    13321 actggattta taggtgttaa tggagcagga aaaacaacaa caatccgttc gatattggat
    13381 gtcgtaaagc gtgatagtgg aaaaataaaa atctttggta aagatttcga tggaaatgag
    13441 aaggaattaa aaaatcgaat tggagttgta tttgatgatt catatttcta tgaggagttg
    13501 tcaatggcag aaatgaaaag tattattgca ccttcatatg ataattggtg tgaagaagat
    13561 tatcaacaaa atatgaagcg ttttaatctt aacccaaaac aaaagattga tacactttca
    13621 aaaggaatga aaatgaaatt tgctttagca ttagcattat cgcataaagc tgagttttta
    13681 attatggatg agcctacaag tggcttagac ccactaatcc gtagtcagat acttgacata
    13741 ctaacagaat atatgaagaa atgtggtcgt ggtgttttct tttccactca tataacatct
    13801 gatttagaca aaatagcaga tatgattgtt atgattgaca atggacgtat tatattagag
    13861 cgtgaaaaag atactctatt ggacgaacat agaaaagtaa aaggagatat tcaatttcta
    13921 aatgatgaaa ccaaaaaatt atttttaaat ttagaaatta ccggctatgg ttttataggg
    13981 attacctcaa aaattaatga agtgcaaaaa aatatgccag atattttaat agagcgtcct
    14041 acaattgaag atattatgct tgcatatatt gaaaggagag agtaattatg ttgattcatt
    14101 tagttaaaaa agatattctg attgtaaaaa aatatgtatt gttgatatta tttattgcaa
    14161 taggaatccc attatttata ttgtggcgtt tacctccatt tgcacaaata ttgggatttg
    14221 tactatccat tatattttca gaatttatgc tgtgccagta tctattatta aaggagagtc
    14281 aatattcaaa agcatctgta ttactttgtt caactcctta tcctagacgt ggcttagttc
    14341 aatctaagta tgttttattt gcaatggtat ttatctattg tacattagtt tactggattg
    14401 aaaatttact tattccacaa ataggaactt ttgacttaac atatattttg attgttttat
    14461 taataagttc tattatttat ggagtttata tgcctattca atataaatta ggatatgaaa
    14521 caacaaaatt ctttttcgtg gtgataatca tggcttcatc atttgtatta ccattaacta
    14581 tagctcacag caattttgaa attccattta tatatcagta tccatccatg atagtgaata
    14641 ttgtattgtt ccttttaagt ttaattatac tgttagcttc aatgtttatt tctattaaaa
    14701 tttttagtaa taaggagctt gtataaggta agggggactt ttatatgcca catttaccag
    14761 attgggctgt tttactttgt atcttacttc caatagtata taaagcttgt cagatattta
    14821 aaagtaaaaa atagagtata tatttatatt gccattttta ttgagtgaag ttagatggta
    14881 tgaaaaggtt acttgaaaat aaagttactt ggtgtgggag agtaacatca taatattata
    14941 gattatatgt ttcttgtgaa tcaatttgta ttttttaata gggaatatcc agtaattttg
    15001 ttttccattg attatttttc aattatgagt atgattgtgt gtattgtata ccatctttta
    15061 aggtgttttt aaaaaattat atcgtcaaaa tataaatcta aggaatccta taaataaagc
    15121 tttttaattt tagaaaacta ataaaaagta ggacgttaat aaaaagaagc tgtctcaaaa
    15181 tagagataag cttctttttc attgctgaaa tgacgaaaat ataattgatt taatataaaa
    15241 aagagtgctt tatgaactat ttattttgaa cagtagatac acttgcactc aatttagtta
    15301 ctcctaagct ttatgaacta aaagtttatt ttgaagctta tttttattaa aagagatttt
    15361 atttttctaa ataatcatct atatacagta ttgcgtttct tttatttatt cataatttat
    15421 tttcttatta aagtttttct atattcatat tttctatatt actttacttt taattttata
    15481 cgaattatca aaaataaaat agttattaga aaaaaataaa agtaattgca aagcttgcaa
    15541 ttacttactt ctatattaat tactatacca taaaataaca aaaaaatcaa gtcgagtaat
    15601 ccttcaactt aaatgatata ctaggtatta ataattttta attaataaaa tataactaga
    15661 ggaaaatcta cattttctca atttctttaa ttaaattgac ctctaattaa gtaattataa
    15721 ggagaaaaat tatgaattta gcaaagggat tagatgtttt aaaaatttca tcaaatgtat
    15781 tcggtgaaga taaagttatg tatattcctg taatctacac agaagatgat gcaacactta
    15841 ttgacacagg tcttccgggt caaggtgatt taataattga tgctcttaat aaaagtaaca
    15901 cgagctttga taagttaaaa aatataataa tcactcatca tgatatagac catataggaa
    15961 acataaatta tctaagagaa aaatctaaga ataatatcaa agtatatgca tacaaaagtg
    16021 aaatcagcta tataacagga gaagaaactc cttttaaact ttatatgttg gaacaaatgg
    16081 tagataaaat tgatgataag atgcttagta tgctaaatgt tatgggactt ggatttaaat
    16141 catcatatac taaagtagat gtgtcattag ataaccatga aaaattgaat ctaggtgagg
    16201 aaatagaagt tatacataca ggaggtcaca ctagaggtca catatgtctt tatttaaaag
    16261 agtctaaagt acttattgct ggagatttgt tacaggtaga aaatggagaa cttaagcctg
    16321 ttgatgtgat gtacagcaac aaacaagagt taaaagatgc tattaaaaat ataagtaatt
    16381 atgatataga aacgatagtt tttagtcatg gtggattata tcaaagaaac ataattgaaa
    16441 cattgaaaaa cctaattatt gagtagtact atatgattta caataatttc ttaaattact
    16501 taaaattata tctaaagatg tagaatcaaa attaaatata agtaataaga attttgcttt
    16561 aaaattatac ttaagtgtta agggtgatac gtagtattac aagtatttta gaatatagtt
    16621 ttttatagta attttaaggt tctacaatac ttcagttgaa aaatatttaa cagc
//
LOCUS       DAGSHT010000062        16621 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGSHT010000062 DAGSHT010000000
VERSION     DAGSHT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.064"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            66..1469
                     /locus_tag="MJB72_001862"
     CDS             66..1469
                     /locus_tag="MJB72_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262970.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            1496..3040
                     /locus_tag="MJB72_001863"
     CDS             1496..3040
                     /locus_tag="MJB72_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBY8262971.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3071..4462
                     /locus_tag="MJB72_001864"
     CDS             3071..4462
                     /locus_tag="MJB72_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBY8262972.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            4655..6151
                     /gene="abfD"
                     /locus_tag="MJB72_001865"
     CDS             4655..6151
                     /gene="abfD"
                     /locus_tag="MJB72_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBY8262973.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            6286..6561
                     /locus_tag="MJB72_001866"
     CDS             6286..6561
                     /locus_tag="MJB72_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262974.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            6585..7892
                     /locus_tag="MJB72_001867"
     CDS             6585..7892
                     /locus_tag="MJB72_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBY8262975.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            7912..9030
                     /locus_tag="MJB72_001868"
     CDS             7912..9030
                     /locus_tag="MJB72_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBY8262976.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            9317..10237
                     /locus_tag="MJB72_001869"
     CDS             9317..10237
                     /locus_tag="MJB72_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBY8262977.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            10352..11509
                     /locus_tag="MJB72_001870"
     CDS             10352..11509
                     /locus_tag="MJB72_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBY8262978.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(11594..13093)
                     /locus_tag="MJB72_001871"
     CDS             complement(11594..13093)
                     /locus_tag="MJB72_001871"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBY8262979.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            13689..15113
                     /locus_tag="MJB72_001872"
     CDS             13689..15113
                     /locus_tag="MJB72_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBY8262980.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            15148..>16621
                     /locus_tag="MJB72_001873"
     CDS             15148..>16621
                     /locus_tag="MJB72_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8262981.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELN"
BASE COUNT         6468 a         1979 c         3112 g         5062 t
ORIGIN      
        1 atagagtatt ggggagaaag gagaaatata ttttttaata atatttgtta atttaataac
       61 gaaaaatgaa agggagtgat ttagtgaagt ttgaattgat ggatttttta atgaatcctt
      121 ttgtccttat gtttgctgca gtaattacag gaattttatt tggaaaaatt aaatttggga
      181 agtttaattt tggggtatca ggagcattat ttacaggatt atttattgga tggcttgcat
      241 atagcttggg gaatctaata atagcaaaag gggaaacagc agcaggatat aaggcagcaa
      301 ctgtaatgat gggtaatgga attatttctt ctgacttctt tgatttcttt ttaataatat
      361 ttgttgcagc agttggtcta ttggcagcaa aagatatgaa agctgtatta aagaagtatg
      421 gagcaagatt tgtaatttta ggagttctta taacttttat aggtggctct atgacttatg
      481 caatgacttt actatcaagt gataaaggaa gtagtgctta tgaagtttct ggcgtttaca
      541 caggagcatt aacaagttcg cctggattag ctgctgcttt agaaacagct ggtaaacatg
      601 cagaagatgt atcaaaagaa tttgaaaaag cttcaataaa agataaaaaa gaaattttaa
      661 aagtagtcga cccagaaggc aaattggatg tgaatacaac tacatcactg actcaagagc
      721 aaatagataa gtatattgca tatgctgaag ctggtgttgg tataggtcat gcagtcgcat
      781 atccttttgg agttttaata gtaatcttag gtgtaaattt ccttccaaaa ttatttagga
      841 tggacctaaa agaagaaaga agaaaatatg agaaggaaat gaaggaagca agagatagtg
      901 tttctggtaa aacgatacca gaagttccat ttaatataat aacatttttc ttgacatgtt
      961 tagctggata cttagttgga ggaatacatg tatttatggg accactaggg tacttcacac
     1021 taggcgcaac aggaggttca ctaattgtat ctctagttct tggatatata ggtaagatag
     1081 gggtagtaaa tttcagaatg gaagaaaagg ttttaaatat tttaaaacaa atagggctag
     1141 tatttttctt agcaatagtt ggattaagat atggtggaaa agtagttgat tcaattatga
     1201 catctggtat gcacctagcc ttagtagcaa tagcagtagg tgttacagct atgatgatag
     1261 gattcttagt tggcaaatac gtatttaaat taaattggat tttattatct ggagctgtct
     1321 gtggtggtat gacatcaaca ccaggtcttg gagctgctgt tgatgctctt gatagtgatg
     1381 acccagcagc aggttatggt gctacgtatc catttgcatt attaactaaa gttatattgg
     1441 ttatagtttt acacaaactt ccaatgtaaa taagatttta tatgggggga tatttatgaa
     1501 taatgaagga gcatttgaag aattaagtgg aagattaaga aataaagaat tgtgtgattt
     1561 aataatgaat gcagatgaag ctgctaaaat tatagaggat ggcatggtta ttggagttag
     1621 tggatttaca ccttcaggct atccaaaagc agtaccatta gcagtttcag aaagagctaa
     1681 aagtggagaa aagataaaat taaccgtata ttcaggagca tctttaggtc cagaagttga
     1741 tggagcatgg tcagaagcag gtataattga aagaagactt ccttatcaaa ctaattcaat
     1801 acttagaaat aatattaata aaggtgttgt tgattatata gatatgcacc taagtcattc
     1861 tactcaattt ttaaattatg gaactatacc taaagtagat gtagctatag tagaagcact
     1921 tgcaataact gaagaaggaa atattatacc aactagtggg ataggtaatt ctccatcttt
     1981 tataaaaagt gcagataaag taatagtaga gataaatctt gcaaaaccaa tggaaatgga
     2041 aggtatggca gatatatata ttacggaaaa tccaccaaat agaaaaccaa tagaaataaa
     2101 tcatcctaag gatagaattg gaacaacata tataccttgt ggtttggata aaattgcagg
     2161 agtagtaatt accaatatgc aagataagac ccgtccattg ggagttgtag atgaagcttc
     2221 gaaaaaaata tcaaataata ttattgcttt tttaagagaa gaagtaaagg caggcagatt
     2281 atcaaagaat ttacttcctc ttcaatctgg tgtaggaagt gtagcaaatg cagtattata
     2341 tggattatgt gaatctgaat ttgaaaattt aacttgttat acagaggttg tacaggactc
     2401 tatgttggat ttaataagaa tgggtaaagt tacaatggca tctactacat ctgtaagtcc
     2461 atctccagaa ggattaataa aatttgaaaa agatattgat ttttttaagg ataagattat
     2521 attaagacct caagaaatta gtaacaatcc agaaatagcc agaagaattg gtgtaattgc
     2581 aatgaacact gcaattgaag ttgatattta tggaaatgta aattctactc acataatggg
     2641 gtcaaagatg atgaatggaa ttggtggttc tggtgatttt gctagaaatg gagcaataac
     2701 tatatttagt actgagtcaa ttgcaaaaaa tggtgatata tcatcaatag tacctatggt
     2761 atctcacgta gaccacactg aacatgatgt aatggtaatt gtaacagaac aaggatatgc
     2821 tgatttaaga ggtcttgcac ctagagaaag agctataaaa ataatagaaa attgtgccca
     2881 tccagattat aaagaacaac ttagggatta tttaaataga gcttgtaaaa gtggagcaaa
     2941 acaaacacct cacatattgg acgaagcttt atcatggcat agtaaattta tgagtacagg
     3001 tacaatgaaa aaggctgaaa cttttaaaag tgcattataa aatatttaat aaattggagg
     3061 tagattttaa atggaaaaag cagtggaaaa ctttgaagat ttaagtaagg aatacattaa
     3121 tggttatatt gagagagcaa gaaaagctca aagagagttt gaatgttata ctcaagaaca
     3181 agtagataaa atagtaaaaa tagttggaaa agtagtgtat tataatgctg aatatttagc
     3241 taaacttgct gtagaagaga ctggaatggg agtatatgaa gataaggtag ctaaaaataa
     3301 aagcaaagca aaagttatat ataacaattt aaaagataaa aaatcagttg gtataattga
     3361 tatagataga gaaactggaa taacaaaggt agctaaacca gttggagtag tagcggcaat
     3421 aactccttgt acaaatccaa tcgttacacc tatgagcaat gcaatgtttg cacttaaggg
     3481 aagaaatgca attattatca cacctcatca taaagcaatt ggatgtagta caaaaactgt
     3541 agagatgatt aatgaagaat tagaaaaaat aggagctcca gaaaatttaa tacaaatact
     3601 tgaccaacaa tcaagagaaa atacaagaaa tttaatttca tcagcagatg tagtaattgc
     3661 cactggagga atgggaatgg taaaagctgc gtactcaagt ggaaaaccag cacttggtgt
     3721 aggggcagga aatgttcaat gtatcataga tagagatgta gacattaaag aagcagtacc
     3781 taaaatcata gcaggaagaa tttttgataa tggtatcatt tgttcaggtg aacagtcagt
     3841 aatagtagca gaagaaatgt ttgataaaat aatggatgaa tttaaaaata ataaaggttt
     3901 tatagttaga gataaagtac aaaaagaagc tttcagaaat gctatgtttg taaataaatc
     3961 tatgaataaa gatgcagtag gtcaatcagt tcatacaatt gcaaaaattg caggtgttga
     4021 gataccagaa gatacaaaaa taatagtaat agaagctgat ggaccaggag aagaggatat
     4081 tattgcaaaa gaaaaaatgt gtccagttat atctgcatac aagtataaaa gttttgaaga
     4141 aggtgtagct attgcaaaag caaatttaaa tgtagaagga aaaggacata gtgtatctat
     4201 acattcaaat acagttaaaa atatagaata tgcaggagaa aatatagaag tatcaagatt
     4261 tgttattaat caatgttgtg ctactagtgc tggaggaagt ttctttaatg gtcttgcacc
     4321 tactaatact ctaggttgtg gttcttgggg aaataacagc atctctgaaa atcttgatta
     4381 taagcatcta attaatattt ctagaattgc ttattatatg ccagaaaatg aagtccctac
     4441 agatgaagaa ctttggggat aaaatttaga caaaaaatat tcattctaaa ttaaagtagg
     4501 aatatatttt aatttagaat gaatttaaga cataaattaa atttaaatta gtttattgga
     4561 ctaaatttaa aatattaaga gacatataga agaaggaagt attataattt aaaataaata
     4621 aaaaaatcta taaaagagaa ggagtggttt tattatggca ttaatgacag gagctcaata
     4681 tattgaaagt ttgagaaaat taaatactaa ggtttatatg tttggtgaag aggttaagaa
     4741 ttgggttgac catcctatga ttagaccatc tataaattgt gtagcagcaa cttatgactt
     4801 agctcatgac ccagaatatg cagatttaat gacagttact tcaaatatca caggtgaaaa
     4861 aataaataga tttggtcatc ttcatcaaag tgttgatgat ttgataaaaa aagtaaaaat
     4921 gcaaagacta tgtggtcaaa aaacagcttc ttgtttccaa agatgtgttg gtatggatgc
     4981 gtttaatgca gtgtattcaa caacatttga gtgtgataaa gcccatggaa ctaattatca
     5041 tgataatttt gtaaaatact taacttatat acaagaaaac gatttagtag ttgatggagc
     5101 tatgactgac cctaagggag atagaagtct atcacctagt gctcaacctg acccagatat
     5161 gttccttcat atagtagaaa gaagagaaga tgggataatt gtaagaggag caaaggcaca
     5221 tcaaactggt tcaataaatt cacatgagca ccttataatg cctactataa gtatgactga
     5281 agcagataaa gactacgctg tatcatttgc agttccttca gatgctgaag gagtgtttat
     5341 gatttatgga agacaatctt gtgacactag aaaattagaa gaaggtgcag atgttgattt
     5401 aggaaataag gagtttggag gtcaagaagc tttagtagta tttgataatg tatttgttcc
     5461 aaatgataga attttcttat gtggagaatg ggatttctct ggtatgttag tagagagatt
     5521 tgcaggatac catagacaaa gttatggtgg atgtaaagta ggagtaggtg atgtaataat
     5581 aggagcagca gctttagcag ctgattataa tggagcaaac aaagcttcac atataaaaga
     5641 taaactaata gaaatgactc accttaatga aagcttatat tgctgtggta tagcatgttc
     5701 atcagaaggt cacaaaacag aagctggaaa ctatcaaata gatttacttc ttgcaaatgt
     5761 gtgtaaacaa aatgtaacta gatttcctta tgaaatagta agacttgcag aagatatagc
     5821 tggagggctt atggttacta tgccttcaga aaaagacttt aaatcagacc ttaaagttgg
     5881 aacaagtgga atgactattg gcgaagtatg taacaagtat tttaaagctt cttcagtggc
     5941 ttcaacagaa gaaagaatga gaatattaag attcttagaa aatatatgcc ttggttcatc
     6001 tgctgttggt tatagaactg agtcaatgca tggggcaggt tcaccacaag ctcaaagaat
     6061 aatgatttca agacaaggaa acataaatca aaagaaagaa ttagctaaga aaattgcggg
     6121 aataaaaaaa gaggaagctt taaatttata aaaagttgat atataaaggg aaattatata
     6181 ttaatgtgtg ttaattaatg aaaaaatatg tagtataaga gatagttata aaattatggt
     6241 ggtttttagc taagtaaaga aatttttaat aaggagaatt tacacatgac ctgtggagtt
     6301 agattttgtg ggggctgtaa ccctagatac gaaagaggga aggctcttac aataatcaaa
     6361 gagcacttca atggtagggt agattttagt atagcacaag agaacgtcga gtatgatttg
     6421 ttacttataa ttggaggatg cgcaactcta tgcgcatcct atcaacaata taaagtgaaa
     6481 aatgaatgtg ttaagatgtg ggattttagc catatagaaa gagttataaa tgtgattgaa
     6541 tcagcaattt gtgaaactta aaaaagctaa atggggggat ttcattgagt tggcaagaat
     6601 tgtatcaaag taaattatgt agtgctacag aagctgtaaa acagattaaa aacggtgata
     6661 ctgtagtatt tgcacattgt gtaggtgaac ctcctgcact agtagaagct atgattgaaa
     6721 atgctgaaca atataaagat gttgagatta aacatatggt aagcttgggt agtggtggat
     6781 atactgctaa gggaatggaa gctcatttta gagtaaatcc aatgtttgta agtggaaatg
     6841 taagaaaggc aattgaaaat ggagatggtg attttacacc tgcattcttc catgaagtac
     6901 caaagttgct tagagaaaaa agattaaaat gtgatgttgt tttagcacag gtaactccac
     6961 cagatgaaca tgggtattgt agcttaggaa catcagttga ttatacttat gaagccatta
     7021 aaactgctag aacagtaatt gttcaagtga atgaccagtt tcctagaacc tatggtgagg
     7081 tagtccatgt atcagagttt gactatatag tggaaaaatc acaaccttta tttgaactac
     7141 aacctgcaaa gattggagaa gtagaagaag caattggtaa aaattgtgct tcattaattg
     7201 aagatggctc tactttacag ctagggattg gtgggatacc agatgcagtt atgttatttt
     7261 taactgataa aaaggattta gggattcatt cagaaatgat ttcagatggg actttggccc
     7321 tttatgaaaa aggtgttata aatggtaaat ataaaaattt tgataaagaa aaaatgacag
     7381 ttaccttctt aatgggtact aaaaaattat atgactttgc aaataataac cctgcagtag
     7441 aggtaaaacc agtagactat gtgaatcatc cagcaattat aatgaaacaa cataagatgg
     7501 tttctataaa ttctgctata caagttgatt taatgggaca ggtagttgca gaggctatgg
     7561 gacttagaca atttagtggt gttggaggtc aagttgactt tatacgtgga gtgtctatgg
     7621 gtgaagatgg aaaggcaatt atagctatgc cttcaataac tacaaaaaaa gatggtacag
     7681 taatttcaaa aatcgtatct attgtggatg aaggtgcacc tataacgaca tcaagaaatg
     7741 atgttgacta tattgtaaca gaatacggta tagcagaatt aaaaggaaaa tctcttagag
     7801 aaagagcaag aaatttaata aatatagctc atccaagtgt aagagaacat ctagcagtag
     7861 aatttgaaaa gagatttaag gagaaatatt aataatagag gggtgttaat aatggaagca
     7921 cttagagtag ttccaaagat attttacttt gatacattta aagaatttaa tgaagaattt
     7981 aaaataggaa aaaatgattt agtaattact aatgagttta tatatgagcc atatatgaaa
     8041 cctcttggaa tagatacaaa tttaattttt caagaaaagt ttggaacagg tgaaccatct
     8101 gatgaaatga tagactccat gacaaaggaa atgaagaaat acaattttga tagaattatt
     8161 gcatttggag gaggaacaat agttgatata tgtaagatat tagcacttga tgttccagaa
     8221 aagtctattg atttatttga aggagaagta tcacctaaaa aagtaaaaga gttagtagta
     8281 gttcctacaa catgtggtac aggtagtgaa gtaacaaatg tagctattgc agagttaaaa
     8341 tctaaacata ctaaaaaggg gcttgcagta gaagaaacat atgcagacta tgcagtgtta
     8401 attccagaga cagtaaaagg gttaccttac aaattctttg ttacaagttc ggtagatgct
     8461 cttatacatg ctattgagtc ttatctttct ccaaaagcaa gtccatttac agagatgtat
     8521 tctctacaag caataaaaat gattatggat gggtataaga agatagttga taaaggagaa
     8581 gaagagagat ttaatcatct tagagacttt gtacttgcat caaattatgc aggaattgct
     8641 tttggaaatg ctggttgtgc agcagttcat gcactatctt attcaatagg tggagctttc
     8701 catgttgcac atggtgaagc aaattatcag ttctttactg aagtatttaa gatgtattct
     8761 agaaaaaaac ctaatggaaa aattataaaa tgtacaaaaa ttttagcaga tgcccttgaa
     8821 tgtgacccta actgtgatgt ttatggagaa ttagaaaagt ttttaaataa attaatagct
     8881 aaaaaagctt tgagagaata tggaatggtg gaaagtcaga tagatgaatt tacagatagt
     8941 acaatagcaa atcaacaacg actacttgct aataattatg tagagcttag tagagaggaa
     9001 ataagagaga tatttgcaaa tctttattaa tacaattttt tatttatatg tatagtcaac
     9061 ataaacaatg ctaattatat ttatgtacaa ataaaaataa gggaacttaa attttaatta
     9121 aagttaagtt ctcttatttt tatatggagt taaatataag tatattttta atttaaaata
     9181 tgcttatatt taagtctatt actttatttg aaacaatttg tagaatttca aattccttga
     9241 cagtaataag taagtaattg tataatcaaa ctgcaatata ttaataatat tagtgaaaat
     9301 gaaaggatta taaaatatgt ataaatcaga aaatttttca aaaaaatctg tattaataaa
     9361 ttcattttta agtaaatttt taaaaataat tggatggggt ttggtaagct tatttacatt
     9421 atcagcctta gtactttcaa ctatttccat tataattgga tgggctgtaa tacttcctat
     9481 tcaagtatgt gcagtatttt tacttatagg aggagtttgt atttttaaag gtaagagatt
     9541 aggagaccaa atttctcgtt ataaaaaata ttgtacaatt ataaacaata gaaatattat
     9601 tccagtagaa cttattgcag aaaacacatc taaatcatta aattttataa taaaagatgt
     9661 acaaaagatg atagacaaaa actactttat aaatacttat attgataaaa gaaataatca
     9721 gattgtgtta acaaatgaag attttgttcc accaagatat gaagatgtag catatgaagt
     9781 caaagaagaa gaaaagactg atgaatccaa tgaagttgat acaattataa ataaaggaat
     9841 gaattatcta acacaaataa aagaagctaa taaaaatatt aaatcagaat caatgggcga
     9901 taaaattgta caagtagaag atgttacaag taaaatattt gatgttgtaa aacatgaccc
     9961 atcaaaactt actcagatac aaaaatttat ggattattat cttccaacca ctttaaaact
    10021 attaaattca tatcatacat tggaagaaca aggaattgat agagaaaata taacaacaac
    10081 tatggaaagt attgaaaata caatgaatac gattgtagta gcttttgaaa atcaattgga
    10141 ctatctgttt gaagatgaag ccattgatat ttctacagat attactgttt tagagaatat
    10201 gttagtccaa gaaggattaa cgagtggtaa attttaactt caatagaaaa aatattctgt
    10261 tatttttaaa caattaatta gtcataaagt ataaaatgtg tattatataa gttaatcagt
    10321 actttatgaa ggaagaggag agaaagatac aatgaataat ttagatgata taccagttat
    10381 gccaactttg acacttgacc catttggaga agaatcaaac actaatgata tagataattc
    10441 tgacttatta atgaaaaagg atgaaaaaga cccagaagaa gaaaaactgt ctgaatctga
    10501 acgcaagatg gtaaaggaat ttgcggaaaa aatagatatt acaaatacaa atatgatttt
    10561 gcaatatgga gctggagctc aaaaaaaggt agcaagtttt tctgaaacag ctttaaagag
    10621 tgtaaaaaca agagatttag gagaagttgg agatatgctt acaaatctag ttactgattt
    10681 aaaagcattt tcagcagatg aaaaagaaca aagtggtttt ttaggaattt ttaaaaaggc
    10741 aaataataaa atttcaaatt taaaaactaa gtatgatagt gcagaggtaa atgtggataa
    10801 agtaagcaaa gagcttcaaa agcatcaggt taaaatattg aaagatatag caatgcttga
    10861 taagatgtat gaattgaact tagcttactc aaaagagctt actatgtata ttatagcagg
    10921 aaaacaaaaa ttaaaggata tgaaagaata tgaaatgcca aagttgagag aaaaagctcg
    10981 tttatctggt tcaacagaag atgctcaatc tgtaaatgat atggtttcac tttgtgacag
    11041 atttgaaaag aagattcatg acttagaatt aactagaatg gtatcgcttc aaatggctcc
    11101 tcagataaga ttggttcaaa atagtaataa tctgatggca gaaaagattc aatcaactat
    11161 agtaaatacc attccattat ggaaaaatca aatagtgctt gctcttggaa tttctcactt
    11221 aaatcaagct atgaaagcac agcgtgaagt tagtaatatg acaaatgagc ttttattaaa
    11281 gaatgcagaa actttgaaga tgggaacaat agaaacagca aaagaatcag aaagaggaat
    11341 tgtagatata gacacaatta agaaaactaa tcaatctcta atatctacta ttgatgaggt
    11401 agttaaaata caacatgatg gtagaataaa gagacaagaa gcagaagttg aattatcaaa
    11461 aatagaaaat gaacttaaaa gtaagcttct tgaatttaca gttaaataaa aattatacta
    11521 atataaaaag agactgtctc aaaatagaga taagtctctt tttcattgtt taaatgatta
    11581 aagtatattt atattataaa tttctattat cttttaacat aacatcatga gctccacctt
    11641 caactatact agtagaagat acaagtgtaa gtttagcatt tttttgaagt tcaggaatgc
    11701 taagtgctcc acagttacac atagtagaac gaactttact taaagaaaga gttacattgt
    11761 ctttcaaaga accagcataa ggcacataag agtcaacacc ttcttcaaaa gaaagtttct
    11821 tatctccacc aaggtcatat ctttgccagt ttctagcacg agcagaacct tctccccaat
    11881 actctttcat ataactacca tttatattaa ctttgttagt tggactctca tcaaaacgag
    11941 agaagtatct accaagcatt ataaaatctg cacccatagc taaagctaaa gttatatgat
    12001 ggtcataaac gataccacca tcagagcaaa ttggaatata aatacctgtt ttttcaaagt
    12061 attcgtctct agcttgagca acttcaatta tagaagtagc ttgtccacgt cctatacctt
    12121 tttgttcacg agttatacat atagaaccac caccaatacc aactttaacg aagtcagcac
    12181 cagcttcagc taggtaaagg aatccttcgc ggtcaactac atttccagct cctaccttaa
    12241 ctgtatcacc atatttttca cgaatgaaat caagtgttat tttttgccac tcagaaaaac
    12301 cttctgaaga atctatacat aaaacatcag caccagcttc aactaaagca ggaacacgtt
    12361 cagcaaagtc tctagtatta ataccagctc caactacata tctcttagaa gaatctaaaa
    12421 gttcaagtgg attttcttta tgagaagagt aatctttacg gaataccata tacataagat
    12481 taccattatc atcaagtaca ggaagagaat taagtttgtg gtcccatata atattgtttg
    12541 cttcctttaa agtaacatct ttgttagcat aaactataga tgacatagga gtcataaatt
    12601 cactaacttt agtatctaaa tccatacgac ttatacgata atcacgactt gcaacaatac
    12661 ccaataattt accagttgaa gttccatctt cagtaacagc tactgtagaa tgtccagttt
    12721 tttcttttaa gtcaagtatg tcttttaatg tgttatcagg tttaatattt gaatcactta
    12781 caacaaaacc agctttgtgg gatttaacac gtgctaccat agcagcttca ttttcaatag
    12841 tttgagaacc gtatataaat gaaataccac cttctttagc aagtgcaata gccattttgt
    12901 catcagaaac agattgcata atagcagaag tcaaaggtat attcatataa atatctgctt
    12961 cctcgccctt tttaaattta gtaacaggtg tttttaaact tacattagca ggtatacaat
    13021 cagtagaaga gaaacctggt acaagaaggt attcactgaa agtatgtgat ggtgtatcaa
    13081 aataaaacgc cattttttaa tcactcctta tatttttata ctttttataa ttgatagtat
    13141 taattataac ttattcttta attttattaa agtattttgt caaatattga gatttttgaa
    13201 aaatttaaac tattttaatt aaatttgttt aatatatatt gccttatagg gtaaagttgt
    13261 atattaaata atgtttagtg tttaatagta gctaatttaa aattaatctt tagtgattca
    13321 tataattaca tttgtatagt ttacattgat aatattacta gagagtgtat aaacctcaaa
    13381 aatacagata aattaaatct atattagtat atccaaaagt acatttaata aatttattta
    13441 actgaattat tattttcatt tctattaaat ttaaatattt tataaaaata gtattattta
    13501 caaaatcata tactccaaaa tactacataa ataaaattaa acaaaaacta aaatgacaaa
    13561 tgtcattatc taaagtgaca tagaaacggt ttcttaaagt tgttactata aaatataaac
    13621 tctataataa aagtataaca aaagggaatc atatcgatat agaatataat tcaagaggag
    13681 gtaaaattat gagtagttct aatcaatcga caataaagac atctatacag cgagtaggga
    13741 aatttttaag tggtatggta atgccaaata taggagcttt tatagcatgg ggtcttataa
    13801 cagcattatt tataccaaca ggatggatgc ctaatgaaaa attgagtact ataggtgacc
    13861 caatgataaa atacctttta ccactattga tagcttatac tggtggtaaa gcaatagcag
    13921 gacaaagagg tggcgtaata ggtgcagcag cagctatggg agtaattgtt ggtgcagata
    13981 taccaatgtt tatcggagca atgataatgg gaccatttgc aggatgggta attaagaagt
    14041 ttgataaatt tgtagatgga aagataccaa caggatttga aatgttagta aataactttt
    14101 caataggtat aataggtatg cttcttgcaa tactaggatt ttatgcaatt ggaccagcaa
    14161 tagtagcagg aacagctctt atagaatctg gagttcaatt tatagttagc aaaagtttat
    14221 tgcctctagt ttcagtattt atagaacctg gaaaagtatt attcttaaac aatgcaataa
    14281 atcatggtat attagggcct ataggtatag ctgaggcaaa agaagcaggt aaatctataa
    14341 tgttcttact agaaagtaac cctggaccag gtttaggagt tctattagca tattggatgt
    14401 ttagtagagg aagtgtaaaa caatcagcac caggagctgt aataatacat ttctttggtg
    14461 gtatccatga aatttacttc ccatatattt taatgaatcc agttttaata ttagctacaa
    14521 tggcaggtgg agcagcagga atattgactt tttcaatatt aggagctggg cttgtagcag
    14581 caccatcacc aggaagtata tttgcactta tggcactagc tccaaaggga ggattacttc
    14641 cagtattagc aggagttgca gtggcaacag ttgtatcatt cttagtggca gcaccatttg
    14701 ttaagagagc ttcagcaaat caaagtgaag aagacagtac ttctttagaa gaagctaaag
    14761 caaaaatgag tgatatgaaa tctgcaagta agaactctga gaaaaatatt gaggaaaaac
    14821 agcttgaagt aaatgagatt aagaagattg tatttgcatg tgatgcagga atgggttcga
    14881 gtgcaatggg agcttccaga tttaaaaata gaataaaaaa cttagactta aatatagaaa
    14941 ttacaaattc atctgttgat aatcttccag atgatacaca aatagtagta actcacaata
    15001 cattagttga aagagttgct aagaataatt caagtgttga gatagtttca ataaacaatt
    15061 ttttaaatga cccaaattta gatacattgt ttaaaagatt agaatctaaa taaaaataaa
    15121 aagtaccact aattaggagt ggataatttg aaaaaaaaga agattacatc taggcaaaaa
    15181 aagataatac taatgatagt agaaaattca aagaaaaaca ttccaataac tatttctgag
    15241 attgcaggga ctttagagtt aagttcaaga actgtactta gagatatgtc tggaattgaa
    15301 aaatggtttg atgaaaatga ttttaacttt gtaaaaaaac caggagtggg attgatttta
    15361 gaggaaaata tagaaaatca aaactttata attgagttgt tagaagaaga aaaaatagaa
    15421 aaagaataca gcaaggaaga aagaaattta attatattat caaagctact agtttctaac
    15481 gagccagtaa aatcctatta ttttgcaaaa atattaaagg tatctgaagg ggtactgaat
    15541 aatgattttg ccttagcaag taaatggttg gaaagatttg atattgagct tgttagaaaa
    15601 ccagggcttg gtgtctattt aaaaggtcaa gaaaaaagct ttagagaggc atatgtaaat
    15661 cttatatacg attcattcaa tgaaaaagaa atactagata tggtcagaaa tataagtgaa
    15721 aatatacaaa cagataaagc aatagaaatt ttaagtgaaa atagactttt aaatttaatg
    15781 gacaggtgca taataagaaa ggttgaagct acattaacaa agaagctttc agatttagat
    15841 gtaaatttag ctgatagtgc atatataggg cttgtagttc atatttcatt agccttacag
    15901 aggataaaaa atggtgaaaa tataactatg gataaagagt ttctaaaaga attatctata
    15961 acagaagagt ttaaattagc taaagaaatc gtaaaaggta tggaaacaga ttttaacatg
    16021 gatataccag tagatgaggt tggatatata actatgcata taagaggtgc aaagcaaagg
    16081 tcatcttcaa accataaggc tttaaactta gatgatatag aaataatgga aattacaaat
    16141 aagatgatag atttggcgga agatgaattt aaaataagtt taaaaaatga cgagaggtta
    16201 tttaaagacc tagcaaatca cttagggcct tctataaata gattgaatat gggattagaa
    16261 ataagaaatc ctcttttaga tgaaataaaa agcaagtatt catatgccta tgatggagtt
    16321 gaaaagattt ctagaattat aaaagataaa ctaaatataa attcaatacc tgaatcagaa
    16381 ataggatata ttgcaatgca ctttgcatca gccatagaaa aaaatcttat gatgaatact
    16441 aatataaata ttgttgtggc atgtcccact ggaataggta cttcaagatt cttatcaact
    16501 aagattgaaa ataaatttcc taatttaaat atacttgaaa ccatatcagc tataaacata
    16561 gatgaagagt atttaaagga aaaagatgtt gatttaatag tgtctacagt tgagttaaat
    16621 a
//
LOCUS       DAGSHT010000063        16618 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGSHT010000063 DAGSHT010000000
VERSION     DAGSHT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.065"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..3520
                     /locus_tag="MJB72_001874"
     CDS             <1..3520
                     /locus_tag="MJB72_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8262982.1"
                     /translation="GISILFIIIIILTCGAVFKFKFKLDNKLKETTHTYLSEFTEHNI
                     INLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRMTISTLDGECY
                     SNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKSGKIVGVLSGI
                     YDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQLFDKDNCDEDD
                     CLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKIVSIQSENISY
                     DVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGGVKTCLKDEYM
                     TMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLEKQDKIEVEY
                     RIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSKRFEIQAINDS
                     IVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKDDRTELINSI
                     NKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDITSAKLLEQE
                     LKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENATKNIIKSVDI
                     YHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNNGKPYKVVGS
                     LTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGALMIIDIDDF
                     KGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDISETRDIINKA
                     EELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALYSAKGKGKNCY
                     TLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEVAVNLILSIV
                     GKHFNVSRMYIFENSDDNRVCKNTFEWCNEGITSEINNLKNICYKDLGNYLLNFNADG
                     LFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSYNRMWTNDEVN
                     TLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYELLFINKKML
                     ESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFEVQANITTTT
                     LKWFDGKDACLIFGSEITSIEAEENT"
     gene            3705..5639
                     /locus_tag="MJB72_001875"
     CDS             3705..5639
                     /locus_tag="MJB72_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBY8262983.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5745..6740
                     /locus_tag="MJB72_001876"
     CDS             5745..6740
                     /locus_tag="MJB72_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8262984.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6712..7497
                     /locus_tag="MJB72_001877"
     CDS             6712..7497
                     /locus_tag="MJB72_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY8262985.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7485..8183
                     /locus_tag="MJB72_001878"
     CDS             7485..8183
                     /locus_tag="MJB72_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8262986.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8306..8779
                     /gene="ybaK"
                     /locus_tag="MJB72_001879"
     CDS             8306..8779
                     /gene="ybaK"
                     /locus_tag="MJB72_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBY8262987.1"
                     /translation="MVKTNAMRILDSNKVDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            8976..9815
                     /locus_tag="MJB72_001880"
     CDS             8976..9815
                     /locus_tag="MJB72_001880"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBY8262988.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            9828..10376
                     /locus_tag="MJB72_001881"
     CDS             9828..10376
                     /locus_tag="MJB72_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBY8262989.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10442..11623
                     /locus_tag="MJB72_001882"
     CDS             10442..11623
                     /locus_tag="MJB72_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBY8262990.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            11796..12059
                     /locus_tag="MJB72_001883"
     CDS             11796..12059
                     /locus_tag="MJB72_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY8262991.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(12188..13030)
                     /locus_tag="MJB72_001884"
     CDS             complement(12188..13030)
                     /locus_tag="MJB72_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY8262992.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            13325..14059
                     /locus_tag="MJB72_001885"
     CDS             13325..14059
                     /locus_tag="MJB72_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8262993.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14069..15202
                     /gene="nagA"
                     /locus_tag="MJB72_001886"
     CDS             14069..15202
                     /gene="nagA"
                     /locus_tag="MJB72_001886"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBY8262994.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQRVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            15301..16050
                     /gene="nagB"
                     /locus_tag="MJB72_001887"
     CDS             15301..16050
                     /gene="nagB"
                     /locus_tag="MJB72_001887"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBY8262995.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            16225..16497
                     /locus_tag="MJB72_001888"
     CDS             16225..16497
                     /locus_tag="MJB72_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8262996.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKNNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         6665 a         1758 c         2944 g         5251 t
ORIGIN      
        1 aggtattagt atcttgttta ttattataat tattttaaca tgtggagctg ttttcaagtt
       61 taaatttaaa ttagataata aattaaaaga aaccacacat acatatttgt ctgagtttac
      121 agaacacaat ataattaatc tcaagacaag acttagtggt caatttgaca tgcttgaatc
      181 tattgctaat ttcataggaa acttgaataa tataaatgat gaaatggtga ttgatttaat
      241 ggattctgat gtaaagcgaa gttctttaat tagaatgact atctctacat tagatgggga
      301 atgttattct aatgatggaa ttacccataa tgttcaagat agagattatt ttaaaaaagc
      361 taaaaatgga gaaaagaata tatcagagcc tttaaaatct attgttaata ataaagaaat
      421 aatagtttta tctaccccag tttataaaag tgggaaaata gttggtgttt taagtggaat
      481 ttatgattca aaaaagttaa atgagttatt aggattatct gcatttgatg aaaaagcaga
      541 tgtttttgtt gcaaagaaaa atggagatat agttgctcaa aaaacaaata aaaatagaaa
      601 gacgctatca tgtatatttc agctttttga caaagataat tgtgatgaag atgattgtct
      661 aaaaactata aaagaaaata tgaaaaatga taagtctgga tattttaatt ataacttaga
      721 taatgaaaaa tttatgttaa actatcagcc acttggagtt aatgattggt atgttttttc
      781 tgttgtgcca aataaaatag tatctattca gtcagagaat atatcttatg atgtatatat
      841 ttttgcatta gaaatactac tagttatatt gatttttata atatatatag catgtattat
      901 aaataataac accaaaatta ttataaatga aaaagaaaaa tttaaagtac tcactgataa
      961 tatactagga ggggttaaaa cctgtttaaa agatgaatat atgactatga catatgtaag
     1021 tcaggggttt ttagagctta caggttactc ttgtgaagat ttaaataaaa tatttaaaaa
     1081 tagatttaaa gatatgattt ataaagatga tttagacaag tttaattgta ttattctaaa
     1141 tcaattagaa aaacaagata aaattgaagt tgaatataga attgtaagaa aagatggaag
     1201 tataatttgg attttggaga gaagtaagtc tataaatgat aataaaggaa gtgagtgtat
     1261 tcagggagtt ttaactgata taacagaact taagaaaata caagaagaat tagattcaaa
     1321 aagatttgaa atacaggcta tcaatgatag tattgttggt ggaatagtga taactgagat
     1381 aaatgatgat tttgatagta tatatttaaa tgaaggttat ttaaatatga ttggatatac
     1441 acgcaaacaa ctggaagatg agctaaataa taaattaatc aaacttattt ttaaagatga
     1501 tagaacagaa ttaattaata gtataaacaa acaactcaaa ttaagtgata catttgcatc
     1561 tgaacaaaga gttgaaaaaa gggatggcac tattatatgg attttattaa aaggaagaaa
     1621 gatgatttat gataatggaa aatctgtact ttgttgtgtt attgtagata ttacttctgc
     1681 taaattatta gagcaagaac ttaaaaagag tgaagaacgt catgaaatca ttctaaagca
     1741 gacgcaaaat attataatag aatgggatat agaaaaagat aaaatgaata tatcacctaa
     1801 ttggtataat aagtttaaat atgaccctat aactgaaaac gcaacaaaaa atataattaa
     1861 atcagtagat atatatcatg atgatatgga taagtttatt tcattaatta aacttacttc
     1921 acaaggtagt gaatatgaag agtgtgaaat tagacttaga actagagata ctatttacgc
     1981 ttggtttagt ataaaggcaa caactttatt tgataataat ggcaaacctt ataaagtagt
     2041 aggaagtttg actgatattg atgaatcaaa acgagaagct gaaaagttaa aagaaagagc
     2101 agaaaaagac cctcttacag atttgtataa taaaaaagca tcacaatcac ttatagaaga
     2161 atacataaca ttggataaaa aaagaaaagg tgctttaatg attattgata ttgatgactt
     2221 taaaggaata aatgataatt taggacattt atatggggat gctgtattaa gtgaaattgc
     2281 aaatgaccta ataaatctat ttagagcaac cgatatagta ggaagaattg gtggagatga
     2341 gtttattgta tttataaaag atatttctga aacaagagat attataaata aagcagaaga
     2401 attggtaaaa acatttgaaa ggtctttttt aggtaaagga cgaaattata aaatatcact
     2461 aagtgtagga attgctaagt ttccacaaga tggtatggac tatatgacac tatttagaaa
     2521 tgcggatatg gctctttact cagccaaagg gaagggtaag aattgttata cattgtatga
     2581 taaaaaaatt gataaagtcc aatatataac taatacaaga aatgcaggag atagtgataa
     2641 tgtagagatg tcttttaaag aacatatcac aaattatata tttgatatac tatatgaatc
     2701 aagtgatata gaagtggcag taaatttaat tctaagtatt gtaggtaaac actttaatgt
     2761 gagtcgcatg tatatatttg aaaattcaga tgataatcga gtatgtaaaa atacatttga
     2821 atggtgtaac gaaggtataa cctcggaaat taataatctt aaaaatattt gttacaagga
     2881 tttaggcaac tatcttttaa attttaatgc agatgggtta ttttattgta atgatattga
     2941 aaatttaggt agtgatgtga aaaaagtact taagccacaa ggcatacgtt caatattaca
     3001 atgtaaaatt ttagagggca atgaatttag agggttcata ggatttgatg aatgttcata
     3061 taatcgtatg tggactaatg atgaagtgaa cactttgaca tttatatcta aaacgctcag
     3121 tatgttttta ttaaaaatgc gtattcagag taagctagaa gagtctttaa caattcaaaa
     3181 atctgtgatg gataatatgg atatatgggc atatgttgtt gacaggaata cctatgaatt
     3241 actatttatc aataaaaaaa tgcttgagtc agcaccaaac actcatgtag gtgatttatg
     3301 ttataaagcg atatggaata atcaagataa accttgtaaa aaatgcccta tgatatattt
     3361 aaaagatggt gatgatactt ataaatcatt catgcataat aaaaagtttg aggtacaggc
     3421 aaatataact acaactactt tgaaatggtt tgatggtaaa gatgcatgtt taatatttgg
     3481 aagtgaaata actagtattg aagctgaaga aaatacttaa aatactgtgt tatatatata
     3541 cttaaaatcg caatttgaat ttattagact taaaatgtct atataaatca tttaagttgt
     3601 ggttttaagt attttttatg atataattaa gcgttaagaa caaaattaaa agatactttt
     3661 gtataaaaaa atatttgtta aatatatatt gagcaggtga taatatggaa aataagtttt
     3721 taccaataag caaacaagat atgatagata gaggatggga agaattagat tttgttctag
     3781 ttacaggaga tgcttatgtg gaccatcata gttttggaac agcaataata tctagagttt
     3841 tagaaagtgc tgggtataaa gttggaataa tagctcaacc agactggaga actacagatg
     3901 attttatgaa gctaggaaaa cctagacttg catttctagt taatgcagga aatatggatt
     3961 caatggtcaa tcattattct gtaagtaaaa agcatagaga aaaggatatg tattctccag
     4021 gtgggaaaat gggttataga ccagatagag ctactatagt atattgcaat aaaataagag
     4081 aagcatacag tgatgttgca atagtaatag gtggaattga agcgagctta agaagatttg
     4141 cacactatga ttattggagt gataaggtta gaaagtctat gttaatagat agtggagcag
     4201 atttattagt ttatggtatg agtgaaaagc agattgtaga agtagccaat gcacttaatg
     4261 atggatatga cccaaaatac attagacata ttgatgggac atgctatatt tcagatacat
     4321 tggaagaaat atatgataaa tatatattaa ttccatcata taaagaaatt tgtgaagata
     4381 aaatgaagta tgtagaagct tttaaaattc aatatgatga gcaagaccca tttagaggta
     4441 atataattgt tcaacctcat ggaagtaagt atctggttca aaataagcca gaaaaaccac
     4501 ttagtagaga agaattagat gaagtatatg gattaccata tcagaagaca taccatcctg
     4561 tatatgaaaa atttggagga ataccagcta tagaagaagt aaaatttagt atagtaagtt
     4621 caagaggctg ttttggtagt tgttcatttt gtgcaataac atttcatcaa ggtagagcag
     4681 tgcaaagtag aagtgaaaaa tcaattattg atgaagcaat aggaataaca aatttagatg
     4741 attttaaagg gtatatacat gatgttggag gtccaacagc taattttaga agaccagcat
     4801 gtaaaaaaca aattactaaa ggtgcttgta aaaatagaca atgcttatct ccatcaccat
     4861 gtaaaaattt agatgcagac cacagtgaat acctacattt acttagagct gtaagaaaat
     4921 tacctaaaat aaaaaaggtg tttgtgcgtt ctggaattag atacgattat gtaatggcag
     4981 ataagaacaa taaatttctt agagagttga tagagcatca tgtaagtggt cagttaaagg
     5041 tagccccaga acatatatca gaagaagttt taaagtatat gcaaaaacca gcaggaaaaa
     5101 cttatgataa atttagacaa aagttttttg ctataaatga agaattaggt aaaaagcaat
     5161 atttgatacc ctatttaatg tctagtcacc ctggttctac cttaaatagc gctatagagt
     5221 tagcagagta tttaagagat actcactacc aaccagagca ggttcaggat ttttatccaa
     5281 ctccagggac tctatctaca accatgtttt atacaggtat tgacccgctt acaatgaagc
     5341 cagtttatgt tcctaaatca aaacgtgaca aggcaatgca aagggcttta ttacaatata
     5401 gagcacctag aaattatgat ttggtatact ctgcgttagt tgaagcagga agagaagact
     5461 taattggttt tggacataga tgtcttataa agccaaaaaa tgaaaagcct tattttaata
     5521 gaaataattc taaaaaaaat gtatctaaag gaacaaataa aaacaaaaaa actaatacaa
     5581 ataacagaag taataaaaat caacaaaaaa gttcaactaa aaaaaagaaa aaacattaaa
     5641 gtataaataa aaatacttaa aaaagaagga ttattcgata aattaacaga aattagtata
     5701 aataaatata attattttaa tgacgttacc tttgggagga agagatgaaa aagtatattt
     5761 ctataatatt attagtggtg ttgactatgg ttgttttggt aggatgttca ccaggaaaag
     5821 ataaccctaa aaataaggag ctatcagttg tcaaacaagt aaaagtagct gttccagatg
     5881 gactaccagc agtggcaata gctaaacttg caaatgaaaa tccagagatt aaagaaggat
     5941 atgaaacagt gtatagcatt gaaaagacac ctgaggcgat ttccactaga gtgatgaaaa
     6001 aagatgcaga tatagctata gttccttcaa acatggctgc aatagcgtat aataaaactt
     6061 catcatataa tattgttgga actgtaggaa tgggttcact ttatttggtg tcaacagaaa
     6121 atataaaaga ttactctgat ttagttggta aagaagttgg atgtactgga aaaggactta
     6181 ctccagacat aactataaaa agtcttttgt tacaaaagga tataaattat tcagatataa
     6241 aatttaatta tgtaaattca gcaagtgaac tggtaccatt attggctact ggtaaagtta
     6301 aaacaggtat tgttccagaa cctgcactta gtgctttgat gtctaaaaat ccagacataa
     6361 aaattataaa aagtttaaat gactcatgga aggaagtaag tggttcaaaa gatggttatc
     6421 ctcaatctac tttaattgta aagtctgaat ttttaagaga taataaagat tttgttgatt
     6481 cttttgtagg tcaattatca aatagtatag attgggcaaa caaaaatcct gaagaattag
     6541 gtgcatattc tgaaaaaata aaaatatcta ctgaatcaaa gataatagga aaatctcttg
     6601 aaagagctaa tttaaaatat attccagtta aggacatgat taaagattat aaaaattact
     6661 atgagaaatt agctaatttt gatgataaga cattaggagg caaagtacca gatgaagcaa
     6721 tatactttgt tgagaaataa aaaaaataaa gaacaattta tagataaaat ggaggtaaca
     6781 ctttcttgtg ttatcctctt atttttatgg caaattattg ctttaaaaat tgataatgat
     6841 atttttttac caacagtagg tcaagtattt aatagtataa aagagattat gctaaattct
     6901 agtttctata tagatatact ttactctatg gggagatgtt tttttagttt tttattggct
     6961 atgatttttg ctattatact ttcaatagtg tcttatttaa atagattttt tagaaactta
     7021 ttaaaaccaa tcaatgcttt gacacgttca ataccaacta tgatattggt agtacttgca
     7081 cttatttggt ttgaaaagga tagtacccct ttcatagttg gatttactat agtttttcct
     7141 atattgtatg ataacgtttt gggcgctata ttgaatatag acaagaactt attagaaatg
     7201 gctaatgtgt ataaaataag gtttatagat aaggtgttaa aaatttattt accagccata
     7261 aaatttcaaa ttatatctat actagtatca acattctctt taagtttgaa agttgctata
     7321 gcaggagagg tatatagtca acctacatat ggtattggaa ctatgataca gactgaaaaa
     7381 attaacttta acacatctgg aatatttgca tggataatta ttgttctttt aatatctgct
     7441 attttacaaa tagctcaaaa gtttttagca aggagagctt ttttatggaa aagataaagt
     7501 tgagtattga aaatataaat aaaagatatg atagtcgtat tatatttaga gactttaata
     7561 ttgattttta tgtaaatgag gtaaattgta tccttggaaa atctggttgt ggaaaaacta
     7621 cactcttgaa cataataagt gggattatta aaaatgacac caacaatcta aatatcaaag
     7681 aaaatttaaa tagagtagga aataagcttg aagctagtta catatttcaa gatgatagac
     7741 ttatagactg gttaacagta gaagaaaata taaagatagt tgtaaataaa tactataata
     7801 aaactcaatt aaataaaatt tgtgatgaat acttggaatt ggttggaatt agtgattata
     7861 aaaaattcta tcctcaaatg cttagtggag gtattagaca aagagtaaat attgctagag
     7921 cttttatata tccatcaaaa aacattataa tggatgaacc ttttaaatct attgatgcaa
     7981 agaatacaca gcttataatg gataatttta gaaatatatt aagaaaagaa aagagaactg
     8041 tgttatttgt aactcataat attgaggaag cattgttttt agcagatagg atatttattt
     8101 taggagactc tccaattaga ataaaaaaaa tattaaaaaa ttctaaggaa ttggaaaaaa
     8161 atgaagtttt gaagttgata tagagttata ataatactgt cgttttaaaa gatatttggt
     8221 taaaatctat aagactatat taaaatagta cagaatattc aaaactgtgt ataatgttcg
     8281 tattttataa aaaggaagtg aatgtatggt taagactaat gctatgagaa tattagattc
     8341 aaataaggtt gattataaag taatgtctta tgaagtgaaa agtgaacatg tggatggtgt
     8401 tgaggtggca catgatatag gaagagatgt aaatgaagtg tataagacat tagtaactca
     8461 aggagttagt aagaatatat atgtatatgt aataccagta catgaaaact tagacttaaa
     8521 aaaggctgca aaagtagcta aagagaaaag tgtagagatg attcatgtaa aagatataaa
     8581 taagcttaca ggttatatta gaggcggatg ttctcctata ggtatgaaaa agttttataa
     8641 aacttttgta aatgaaagtg caaagaattt agatacaatt attgtgagtg caggaaaaat
     8701 aggatatcaa atagaactat ctccatttga tttacaaaga cttataaaag tagaatttgt
     8761 agatgtaata aaaaaataag aattacatat aaaatataga gcttatattc tcaataatag
     8821 gtgttgtaat attaataaaa tgaataagaa ttttaaaata aatattcagc tacttatgtg
     8881 taattggtat aaaaatattg agaaatttta aaaacaatgg taaagtgtaa gtataatcac
     8941 gaatattttt tataaagtaa tggagggatg ttcgcttgag aaaaataaaa tctgaacaaa
     9001 tcgtcgagca ggtaaaaaaa ctttgtatag aagcgagcct atatttagga gaagatgttt
     9061 taagttgtat aaaagaaaaa gcgaaaagcg aaaagagtga agttggaaaa aatatattga
     9121 atatattagt tgaaaatgct gaaatagcta aagaaaaaaa tataccaata tgccaagata
     9181 ctggtatggc tgtatttttt gttgaaatag gacaagaagt tttaattgaa ggagacacac
     9241 ttactgatgc tataaatgag ggagtaagac aaggatatga agaaggctac ttaagaaaat
     9301 ctgttgtaag tccaattaac agagtaaata caaaagataa tacacctgct gttattcatt
     9361 atgatatggt aaaaggtgat aaaattaaaa tagaatttgc agctaaggga tttggaagcg
     9421 aaaatatgag taagatgaaa atgttaaagc catcagatgg attggaagga attaaaaaat
     9481 tcataataga tacagtttca gaagctggac ctaacccatg ccctcctatg gtaattggag
     9541 ttggtatagg tgggactgtg gacaaatgtg ctcaaatagc taaaaaggca ctttttagag
     9601 aactaggtga atttaataag gacgaaaata tagcaaaact tgaaagtgaa ctattaacag
     9661 ctattaataa acttggtata ggacctcaag gcttaggtgg tactacaaca gctttgggat
     9721 taaacataga gacttttcct acacacatag caggtttacc tgttgttgta aatataaact
     9781 gccatgcatc aagacataag aaggtagtaa tttaggagga aaataaaatg ataaaaataa
     9841 ctacacctgt aaatgaaatt gatatagcaa aattaaattg tggagataca attagtttga
     9901 gtggtatact ttatacagca agagatgctg ctcataaaag attgatagat tgtataaata
     9961 aaggtgaaga attaccattt gacgtatatg gtcaaggaat atattatgta ggacctactc
    10021 ctacaaagcc aggagaagtt ataggtgctg caggtccaac gacgagttat agaatggatg
    10081 atttgactat acctctttta gaaagaggtc ttagattgat gataggcaag ggaaaaagaa
    10141 gtgatgaggt tatagaaggt atgcaaaaat acggagcagt ttatcttgct gcaataggag
    10201 gagctggagc gtatatttca aattcaataa aatcatgtga aattattgca tatgaagatt
    10261 taggagctga agctataaga aagattgtag tagaagacct taagcttaca gtagcaatag
    10321 atagctatgg aaataatata tatgagcaag gtcgagcaat ttatgaatgt aaataatgtt
    10381 tagaaataaa tatgaatatc aaaattaaaa atagttgtgt atattttaag gaggaaaatt
    10441 gatgtctaag gattatgcaa aattggcatt agaaatgcat gaaatcaaca aagggaaagt
    10501 aagtgtagaa agcaaggtag aaattaaaac taaggatgat ttatcaacag catatacacc
    10561 aggggttgct gaaccttgtt taaaaataca tgaaaatcaa gatgatgttt atagatacac
    10621 ttctaaaggg aatctagtag cagtagttag tgatggaagt gctgtacttg gattaggtaa
    10681 cattggtgct gaagcatcta tacctgttat ggaaggtaaa gctatattat ttaaacaatt
    10741 tgcagatgta gacgcttttc caatatgttt aaagactaat gatgtagatg agatagttaa
    10801 atctgtagag ttgatggagc ctgtatttgg aggtataaat ttggaagata tatcttctcc
    10861 aagatgtttt gagatagaag agagattaaa aaaatcttta agtatacctg tttttcatga
    10921 tgaccaacat gggacagcaa taatagttgc agcagccata ataaactcaa taaagttaat
    10981 tgaaaataaa aaaatagaag atttagaaat agttataaac ggagcaggag ctgctggtat
    11041 agccatagca aagatactac ttaatatgaa tgttaaaaat ataattttat gtgatagaag
    11101 tggagcattg gaagctagta tagaaaatct aaactatgtt caaaaagaaa tgttaaaagt
    11161 aacaaatata agaaatgaaa aaggaccttt aaaagatatt ataaaaggaa aagatgtatt
    11221 tattggtgtt tcaggtccag gagctgttac aaaggaaatg gttgaaagta tgtctgagaa
    11281 gccaataata ttggcaatgg caaatcctac tccagagata atgccagaag aagcaaaatt
    11341 aggtggagca tttataatgg gaacaggtcg ttcagacttc cctaatcaag tcaataatgt
    11401 actggctttt cctggaatat ttagaggtgc acttgatgtt aaggctaaag agataaatga
    11461 agaaatgaag atagctgctg catatgcaat tgctaataca ataagtgatg aagaaatatg
    11521 cccagagtat atattaccag atgtatttaa taagaatgta gtaaaaaatg ttgctaaggc
    11581 agttaaagaa gcagcgataa aaaccaaagt aaatagaata taatttttta caatttatta
    11641 aaaactaaac aagtgtaaaa gactaattaa attatgtcca gtggaatgat tttattagtc
    11701 tttttatttt taatatgaca taataatgtc atattagtct tgatataatt tgaatatggt
    11761 taagagccaa agaaaaatat aaggagtgat ttaaaatgga agaaactaaa ttttgccaat
    11821 gctgtgcaat gccaatggga caaactgatg aattatatgg gacaaataaa gatggaagta
    11881 aaagtactga ttattgtagt tattgtttta aagatggtga gtttacagct gatataagta
    11941 tggaagagat gatagaagtt tgtatccctc atatgcttca aagtaataag gatatgactg
    12001 aagatgaagc aagaacaatg atgaacaatt tctttccaac attaaaacgc tggaaataat
    12061 tttatgcaaa attagttata tgtaaaacat gaaatatggt gttgagaata aggggtggtg
    12121 tgctttatag cataccacct gttttgttac ttggagttca gttattttcc taaatatttt
    12181 aaatatacta ttttagataa gttccctctt catcttgata aattaatcct tttttcataa
    12241 gcactccaag agctcgttta aaatttttct tacttgaatt aaataatata gaaatatctt
    12301 tagggtcaga tttatcatta aatctcatat atccatcaga accttctaaa taacttaaaa
    12361 tcttattttc taaagtatca agttcatcct ttctatttcc tctaggagaa agaccaagtt
    12421 ttccatcttc atatattttt atgacatgta aattttcaag aacatcacca gtttttagtt
    12481 cagtaaagta ctcattatga agtattacac cagcatattt attgtctaca catatcattg
    12541 cagaattatt agtttggaat ccatatacaa ctccagttac aatatctcct acattgtaag
    12601 tatggtctgt taataagtat gaatctatgt ctgtagtagc agcaattctt ttgcttttgt
    12661 ctaaatatat ataaaataaa tatttttcgt ctctaaatag tggataagtc ttagctttaa
    12721 aaggaactaa tatgtcttta ggaagtccca tatctataaa agtaccaatg tctgtattat
    12781 caactacttt taaatgtgct acatccccaa ccttagctaa aggcggtatt aaagttgcag
    12841 cagttctgtc atcagaatct ttaaatatga aagcatttac ttcatcacct atgtttattt
    12901 cattttttcc gattaatcta ttatgaagaa gtatatcgtc ttttgtatta ttagtttgtc
    12961 catccaaaaa atatccaaat tcagtttttc tttttactgt aagtttatta aaatcaccta
    13021 tgtttatcaa attaaaatca tcctctcatt ttatatttat aatttagcca taatatactt
    13081 atgtagtagt ttgtcacagt atctatatta gtattagtga aatttacaat aaataatata
    13141 gttatttatg tatttttcaa ttctctagct tcttatatag tataaactcc aatttctttt
    13201 ttgtctttag gttttattaa aatttttaca aaataagtta aaaagtattg aaataaaatt
    13261 tcttttaaag tataattggt atataccaaa attggtatat accatatcga aaagtggtga
    13321 ctatatgaaa atagtaaata aatctagtaa tataccattg catactcaat taagttcaat
    13381 aataagagag atgatagaaa ctggtgagct taaagagggt gatgcaataa tgccagaaag
    13441 agaactttgc aacatacaaa atgtaagtcg tatgacagta aataaaacca ttgttggatt
    13501 agttacagaa ggacttcttt atagggtaca aggtaaaggg acattcgttg caaagcaaaa
    13561 aaagaagtat cagttttcaa atgtaaaagg ttttactgat gttatgaaag aaaagggagt
    13621 aaacataaaa acggatatat tatcctttga gatggaacta ccagatgatt tagttaaaag
    13681 aaagcttgga ataagtgata atactacaaa tatatataag attgtcagac ttagatatac
    13741 tgatggagag ccatttggat tagaaatagt atatctatca gaagaaatgt gtaagggtct
    13801 aacaaaaggg atattggata attcttcatt atatagggtt ttaaatgaaa aatatggcta
    13861 taagatacaa aaagctgaac aggtaatgga gccagtaata ctttcagatg aagaaagtaa
    13921 gcttcttgaa acagatgaag gtgctttagc tcttaaactt catagaaatt cctataatag
    13981 agaaggtagc cctatagaat ataccatatc aatatttaga acagataaat atcaatatga
    14041 aatagtattg agtgagtagg tgaataatat gaaatgttta attaatggaa agataatatt
    14101 aaaaaatcaa atcttagaaa ataaagtatt agtatttgat gaaaaaataa ttgatatagc
    14161 tgatagtgta ccaaaagatt gtgaagttat agatgctgat ggaaaatata tatctccagg
    14221 gcttattgat attcatatac atggaaacat gggaaaagat acaatggatt cgactgatga
    14281 atctattgaa acaatttcaa aatcaattat gagacatgga gtaacatctt ttctgccaac
    14341 tacaatgact atggataaag aacatgttta tgatgcactt gaagttatta aaaaagccca
    14401 aaatagaaag cttgaagggg cacaagtatt aggagcacat ttagaaggac cttttataaa
    14461 tgagaattat aagggagcac aaaatgaaaa atttataata aattcaaaat acgaatttat
    14521 aaaagaatat aaagatgtta taaaagttat aacttatgct ccagaaaaag atattgattt
    14581 tgattttact agagaaataa aaagatgtac agatatagtt ttatctattg gtcatagtaa
    14641 tgcaaattat gaccaagcta aagaagctat aaatttagga gtaactaatg taactcacat
    14701 gttcaatgca atgactggtt taaaccatag agacccaggc gttgttggtg cagcacttac
    14761 aactaatgtg tacagtgagt taatagcaga tacaatccat ataaataaag atttatttca
    14821 atttatacta aataataaag gaaaagaaag actgatttta ataacagatt ctatagaagc
    14881 tggtggtctt gaagatggca attattctct tggaggtcag agagtcatag ttaagggtaa
    14941 tgaagctaga cttgaaaatg gagcattagc tggaagtgtg ttgtctttaa ataaaatggt
    15001 atttaacttc ttagataata caaaccttaa agtgaatgaa gcaattaatt tagcttcttt
    15061 aaatccagca acatctttgg gaataaatga caaaaaagga agcttagaaa taggtaagga
    15121 tgcagatata gctgtatttg atgagaattt agattgtaaa atgacacttt gcttggggga
    15181 cgtagtatat aaaaatatat agtatacaaa acaaacctta aagacagtaa aaacaatata
    15241 aaaaataata aattaaactt acattagtaa aagtagtagt taaaatttag gaggaataag
    15301 atgagagtat tagtgtgtaa agattatgat ggaatgagta aaaaagccgc tgaaatgata
    15361 gccgctcaaa tagttttaaa accaaattca atactgggac tagctacagg aagtacacca
    15421 gtaggtatgt atagagattt agtaaaaaaa tacaatgata atatagtaga tttttcagat
    15481 gtgatgagct ttaatttaga tgaatattat aagttaccaa tatctaatga ccaaagctat
    15541 gattatttta tgaaagagaa tctatttaat catgtaaaca taaagccaga aaatactcat
    15601 ctaccaaatg gaatggctga tgatattgaa aaagagtgta tgaactatga agcttctata
    15661 gatgcagctg gtggtataga tgtacaagta cttggaatag gtcgtaatgc tcatataggt
    15721 tttaatgaac ctgatactaa atttgcaaaa agaactcatg tagttgaact tacagagtct
    15781 acaatagaag ctaatgcaag attctttaaa tcaagagaag atgttcctaa aaaagctgta
    15841 agtatgggaa taggttcaat attgaagtct aagaaaatat tgcttttagc ttcaggagaa
    15901 gaaaaagctg atgcagtata taatactgtt tatggagata taactccaga agttcctggt
    15961 tctatacttc aacttcataa agacactata gttatagtag atgaagcagc agcaagtaaa
    16021 ttgaatccaa aagattataa attagtataa gaatagaatg tggtttaagt aaaaaatagc
    16081 tatcattgaa taatcaaatt tatttaatga tagctatttt tatttaaatt ttttacttct
    16141 ttttatttaa attagttatt attgtttaaa ttacttgctt attacttttt acgaaaactc
    16201 gttatcaaga agtatccgaa taagatgtta aaaattatta tgtttagtgg gattctatca
    16261 agcataataa aattacctat aattgggata ttaccaactg gaaacataaa gaataataca
    16321 ggcatcagtg atagtaaaaa agatggaaaa caaagaagaa gtaatcttgg aatatcaata
    16381 taccaactac catccttttt gtattctcta atcaataatt cagttcctag taataaacca
    16441 ataccaccag aggaaattaa attaataaaa atgtatcttt taaagtcttg catataacca
    16501 gtgtaaaatt gagtatagaa atcataattt aatttaacgg ctaaaaaata cataagcaat
    16561 agaatgatta aaaataaaag atatctaaga gcttttttca ttttaaattc tcccttca
//
LOCUS       DAGSHT010000064        16419 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGSHT010000064 DAGSHT010000000
VERSION     DAGSHT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.066"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1176)
                     /locus_tag="MJB72_001889"
     CDS             complement(<1..1176)
                     /locus_tag="MJB72_001889"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBY8262997.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     "
     gene            complement(1199..1864)
                     /locus_tag="MJB72_001890"
     CDS             complement(1199..1864)
                     /locus_tag="MJB72_001890"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBY8262998.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(1898..3010)
                     /gene="ribD"
                     /locus_tag="MJB72_001891"
     CDS             complement(1898..3010)
                     /gene="ribD"
                     /locus_tag="MJB72_001891"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBY8262999.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3142..3263)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3452..5935
                     /gene="recQ"
                     /locus_tag="MJB72_001892"
     CDS             3452..5935
                     /gene="recQ"
                     /locus_tag="MJB72_001892"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBY8263000.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6024..6135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6277..7578
                     /gene="thiC"
                     /locus_tag="MJB72_001893"
     CDS             6277..7578
                     /gene="thiC"
                     /locus_tag="MJB72_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBY8263001.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7632..7826
                     /gene="thiS"
                     /locus_tag="MJB72_001894"
     CDS             7632..7826
                     /gene="thiS"
                     /locus_tag="MJB72_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBY8263002.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7819..8622
                     /gene="thiF"
                     /locus_tag="MJB72_001895"
     CDS             7819..8622
                     /gene="thiF"
                     /locus_tag="MJB72_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBY8263003.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8685..9455
                     /locus_tag="MJB72_001896"
     CDS             8685..9455
                     /locus_tag="MJB72_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBY8263004.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9472..10575
                     /gene="thiH"
                     /locus_tag="MJB72_001897"
     CDS             9472..10575
                     /gene="thiH"
                     /locus_tag="MJB72_001897"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBY8263005.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10576..11265
                     /locus_tag="MJB72_001898"
     CDS             10576..11265
                     /locus_tag="MJB72_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBY8263006.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11453..12847
                     /gene="yfcC"
                     /locus_tag="MJB72_001899"
     CDS             11453..12847
                     /gene="yfcC"
                     /locus_tag="MJB72_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBY8263007.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            13364..14731
                     /locus_tag="MJB72_001900"
     CDS             13364..14731
                     /locus_tag="MJB72_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBY8263008.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            14756..15364
                     /locus_tag="MJB72_001901"
     CDS             14756..15364
                     /locus_tag="MJB72_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBY8263009.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            15412..15783
                     /locus_tag="MJB72_001902"
     CDS             15412..15783
                     /locus_tag="MJB72_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBY8263010.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            15962..16300
                     /locus_tag="MJB72_001903"
     CDS             15962..16300
                     /locus_tag="MJB72_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBY8263011.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         6333 a         1815 c         2800 g         5471 t
ORIGIN      
        1 atgtcccatt ttatcttttt tagtttttaa atatagctta ttaaagtcat taatttcttc
       61 attaataggg attctatttt cgatttttat tccatatttc tctaattgat ttattttatc
      121 tgggttatta cttaaaagat ttatactttt tatgtttaaa ttttttaata tttgagcagc
      181 catatagaaa tctctcatat cttcttcaaa tccaagcatt aaatttgctt caacagtatc
      241 gtaaccttct tcttgaagtt tataagcctt aattttattt aaaagaccaa tacctctacc
      301 ttcttgcttc atataaatta aaactccact tccattttcc acaattttct tcatagaaga
      361 ttctagctgt aatccacaat cacattttag agagtggaac acatctcctg ttaaacattc
      421 agaatgaagt ctaactagag tattttctat atttatattt ccataagtaa gggcaatgtg
      481 ttcttcattt gaataagtat ctctatatcc tatgatttca aaatttccat attttgtcgg
      541 aaggaaagca ctagatactt tttctatagt agtttcattt attttgcggt attctataag
      601 gtcttttata gtgatgattt ttaaattatg cttttctttg aaaatcatta aatcatcaac
      661 tcttgccatt gtaccatcat ctttcattat ttcacagata agaccaatct ctttaaaacc
      721 tgcaagtcta gcaaggtcta ctgaggcttc tgtatgtcca tccctgacaa gaaccccacc
      781 agtttttgct attaaaggaa agatgtgacc aggcgttttg aaatctttac ttgtagaatt
      841 ttcatcacag aataatttta cagtttcagc tctttcatat gcagatatac cagttagagt
      901 atctgctcca tctatagtaa cagtaaaggc tgtttgaaat gtatctgtat tttctctaac
      961 cataagaggt atattaagac ttttaaattt tttttcagtg gcaggtagac aaattagacc
     1021 tctaccatat gtagccataa aatttattga ttcaggagta gccatttcag ctgccatcaa
     1081 taaatctcct tcattctctc tactttcatc atcaaccacg attaccattt tcccattttt
     1141 tatatcttca atagcttctt caatagtatt aaacatgtaa ttcactcctt tattattttt
     1201 aaaaaccatt tttaaataaa aaatctttat ctatatttga tttatggtta ttactattag
     1261 aatttaattc cttgtagtta ttagccataa aattattaac atactttcca attacatcgc
     1321 tttctaaatt aacgaaatct ccaacattct ttgtcaagag aatagtgtta attttagtat
     1381 gaggtataat agaaacctta aatatttcat catctaaata tgatatagtt aaacttacac
     1441 catcaatagc aacagaacct ttgagtatca ttgaggataa aagtttttta tcaggtcgta
     1501 tacttactaa aactgcattt tcatcttttt caaatttgca gatagttcct ttaccatcaa
     1561 catgacctgt tactatatga ccaccaaatc ttgaagaaag actcatagct ctttcaagat
     1621 ttaccttgtc atttgcattt aataaaccta aatttgtgga acgaattgtc tccatcataa
     1681 catcggctgt aaattcattt tttccgaggt ttgaaacagt tagacaaact ccatttacgg
     1741 ctatactatc tccaagattt gtgccatcta atattttatt tgatagtatg gttacttttc
     1801 cactcttacc attagcagta atttttctaa gtattccaac ttcttctact atgccagtaa
     1861 acatcctaac ctcctatatg aatgtattaa caaaaattta atttaaaaca tctgattcta
     1921 taagaatatc gccatctatc aaagttacat tattgattgt taactgtgtt gaatctgaca
     1981 aatgatttat tccttctcca gaaacgaatg taggtgcatc tttaccacct ataatcttgg
     2041 gtgatagaaa taatttgacc ttatcaacta acttatttct aaagagactg tcattgagag
     2101 tagcaccacc ttcaactaga acagaatcaa tgtttaactc tcctaattta tacattacaa
     2161 actccaaatc tactaagttg ttttttgaag gagaaattat tgtttcaaca ttattttctt
     2221 ttaacataga taattttata ctttctttgc aacatgtgaa aactatagtt ttactggttt
     2281 tatctttaac caaattacta ttaagtggca actttaggtg tgtatctatt actattcttg
     2341 taggatgaga cactttttct tgattgagcc tacaagttag taaaggatta tcgttcaaaa
     2401 ctgtatttat gccaaccatt atactttggt atttatttct gtatttatga gttagatacc
     2461 ttgatgattc attagatatc cattttgatt ctagtgattt tgtagcaatt ttaccatcta
     2521 gacttactgc tgattttaca atacaaagag gtcttttgtt ttttatatga taaaaaaatg
     2581 cctcatttaa atctcttgct tcatcttcta atattcccac agtaacatca atattgttat
     2641 cttttaattt ttttactcca ttaccacaaa caagtggatt tgggtctaat gtagatatta
     2701 caactctctt aatcttattt tgtataattt tatctacaca aggaggcgtt ttcccataat
     2761 gagaacatgg ttctaagttt acataaagag tagagtcttt tagactttgt ttagcactgt
     2821 ttatagcatt tacttcagca tgattagagc caaatttctc atgatatcct ttgcctatta
     2881 tattactagc tttaacaatt acacacccaa ctaatggatt tggatttaca aagccttctc
     2941 catttttagc caattcaata gcttttttca tataataaat atctttttct ttttgattca
     3001 caaaattcac aatatgtaca cctcctgttt aattggataa tttttaaaca aaaaaccctg
     3061 agaattaact cagggtttag aataatctac aataaatgtt taataaatgt agaacaaaat
     3121 tgctctacaa aaacactcat taaatatctt cttccatcca gactttactg tcggctttgg
     3181 aatttaacca aatcaaccaa gttttacctt ggctcgcggg ctataccgcc gattgggaat
     3241 ttcaccctac cccgaagatt aagtattaaa tttatattta gaatgtatca taaattattt
     3301 tactttgcaa actatttttt taaaaaatat atgttaatat ataatataga atgtctataa
     3361 actttgaaat accacgattg tgaatataaa aataaaaatt tgaacataat caggaaagaa
     3421 gtctaattaa tttaaattag gagagtaata tatgaataaa aaacttttag aaatactatc
     3481 aaaatactat ggatatacaa gttttagaaa aggtcaagaa tctataataa actctatctt
     3541 aagcgaaaaa gatgtgttag ctattatgcc aacaggaggg ggaaaatcaa tatgttatca
     3601 aattccagct ttgattttag atggaatgac aatagtaatt tctccactta tatcacttat
     3661 gaaagaccaa gtagatgcct taaagactat gggaattagt gctgctttta taaatagttc
     3721 attaagtagc aaagaattta atgaaatatt aaataatatt agaaaaaata attataaaat
     3781 actctatatt gctccagaga gattagatgc acaagagttt attgagctaa taaataataa
     3841 caatgtaagt caagttgcga tagatgaagc acattgtgta tctcaatggg ggcatgattt
     3901 taggttgagt tatagaagga tttctgattt tataaaaaat ttgcctaaaa gacctatagt
     3961 tacagcattt actgcaacag cttcagaaga ggtaagaact gatataataa atttactgtg
     4021 tttagaaaat ccagattatt atataactgg atttgataga gagaatttat caataaatat
     4081 agtaaaatca tctagtaaaa ataagtatat tttagattat attcaaaatc ataaaaatga
     4141 gtctggaatt atatatgttg caactagaaa ggaagttgaa aatatatata atggtttatc
     4201 taagagaaat atatctgtgt caaaatatca tgcaggttta tctaaaaatg aaagaagtaa
     4261 aaatcaagaa gattttataa atgataatgt ggatataatg gtagcaacaa atgcatttgg
     4321 aatgggtatt gataaaccta atataagatg ggttatacat tacaatatgc ctcaaagtat
     4381 agaaaactac tatcaagaaa taggtagagc tggtagggat ggagaaaaaa gtgagtgtgt
     4441 acttttattt tctccaggag atgtacatat acaaaaatat ttaattgata ttggagttga
     4501 aaatcctgaa agaaaattat ttcagcataa gaagcttcaa tacatgattg acttagttta
     4561 cagtaattca tgctatagaa agacaatatt gagttatttt ggagaaaatt atatagacaa
     4621 ttgcaataat tgtagcaatt gtttagtaga gggtgaaata gtagataaaa ctatagatgc
     4681 acaaaaagtc atttcatgtg tagctagaat gaaaagaagt tatggtgtta ctactattgt
     4741 agatgtgctt agaggttcta agaataaaaa gattttacag cttggattaa atactttgtc
     4801 tacatataac attatgagag attattctag tgaagactta aagaattttg taaatacatt
     4861 ggtttctcat ggttttttag atttagttga gactctaggc aatggaaata gaggtagttt
     4921 tcctactata agattaaatg agatgtctat gaaagtttta aaaggtgaag tgaaagtaga
     4981 gtttaaagaa attgttatga caaaatcatt ggaagtagag gatgagttat ataatgcttt
     5041 aagagaactt agacattcaa ttgcaagtga agaaaggata gctccatata tggtatttgg
     5101 agatggtaca cttagagcca tgtctagtag ttatccaatt aataaagaag aaatgctaga
     5161 tatctctgga gtaggagaaa ttaaatatca aaaatatggt agagattttg aagcagtaat
     5221 taaagaatat gtcgaaaaaa ataatataaa taaaaaaata gatgaaaaaa atgtcgattc
     5281 aaagaacgtt aatagtgatt tcttagaggt taatacagat aaagttttat atgaaaaact
     5341 gttagtcatt agagaagaat atgcaacaaa ggaaaaatta cctgcatata tgattttagc
     5401 caataaggct ttgaaagaaa taagtggaag atatccactt gatgaagaac aattaaaaga
     5461 tatttctggc ataggtggag taaaatttga aaaatatgga gctagtattt tggcagaggt
     5521 taataaatat gtaaaagaaa ataatattaa tgttatttgg gagaaaaagg gcaaaaggaa
     5581 gttaatatta gatggagaaa gtagaaaaaa taatgaaata gcattagatt tgctaaatca
     5641 aggaaaagat atacaaagta tcagtcaaga aatagaagtt tcactgtcaa ccattattgg
     5701 atatgtgcat gactatgtaa aggatggaca tagtatatca tttgaattag gactagaaaa
     5761 tttttataat gaagataaaa agaagatgat tttagaagtt tgcgaaaaag ttggctatga
     5821 taatttaaat aacataaaaa gaaaacttcc agattcaata aagtatgaaa atataagagc
     5881 tgtgatactt gactcttatc ttgaaaactt aaaaaataat attatagcag agtaattttt
     5941 aacttaataa aaaataaaat ttgtattatt gtgttgaact aataattttg ctgtgttata
     6001 ctgaatttgt aaaaataatg aaaaattaga gctaggggcg ctagaattgg ctagctgaga
     6061 gataaagccg taacttacaa accctaataa cctgatcaag ataatgcttg cgaagggaag
     6121 ctttttaata taattttatt atataaatag gcaaattatt caatgttagg agaaatttgt
     6181 ctaagtgtat atacgattat aagagtgctt tccaaacgag gaaaagcact ttttttattt
     6241 tcaaaaacta taactttaat cttggaggat tgtaatatga actatacaac acaaatggat
     6301 gctgctagaa aaggcataat aactaaagaa atggaaatcg tttctcaaaa agaacaggta
     6361 gatgtaaatg aattaagaga attaatagca aatggacaag ttgttatacc agctaataaa
     6421 aatcataaat ctttaagtgc agaaggtgta ggtaaaaatt taagaacaaa aataaatgta
     6481 aatctaggaa tttcaagaga ttgtaaggat atagaaaaag aacttgaaaa agttagagta
     6541 gcaatagaca tgaaagctga agctataatg gatttaagta attatggcaa aactagagaa
     6601 tttagagaaa aggttgttga aatgtcacct gcaatgatag gttcagtacc tatgtatgat
     6661 gcagtaggat atttggaaaa agaattaaaa gatataacag aagaagagtt tttaaatgta
     6721 ataagacaac atgctattga tggagtagac tttattacta tacatgcagg tcttacaaga
     6781 agtgtttgcc aaaaaataaa aaatcatgag agattgactc atatagtatc tcgtggaggt
     6841 tctttattgt ttgcatggat ggaattaaat aataaggaaa atcctattta tactaatttt
     6901 gataaaatat tggatatatg tgaagaatat gatgttacat tgagtttagg agatgcttgt
     6961 agaccaggtt gtataaaaga ttctactgat ggtgttcaaa ttcaagaact tgtagtatta
     7021 ggtgaactta ctaaaagagc atgggaaaga aatgtacaag taatgataga aggtcctgga
     7081 cacatggcta ttgatgaaat agaagcaaat gtagtattag aaaagagatt atgccatgga
     7141 gcaccttttt atgttcttgg accattggtt acagatatag caccaggtta tgaccatata
     7201 acaagtgcaa taggaggagc tttagcatgt gccaaaggag tagatttttt atgttatgta
     7261 actccagcag aacatttaag attgcctaat ttagatgata tgaaagaggg tataatagct
     7321 gcaaagatag ctgctcatgc aggagatata gctaaaaatg ttaaaggtgc tcgtgaatgg
     7381 gataataaga tgagtaaagc tagagctgat ttagactggt gtgagatgtt tagacttgca
     7441 atagaccctg aaaaagctaa gagatacaga gatgaatcaa ctcctactca tgaggatagt
     7501 tgtactatgt gtggaaaaat gtgctcaatg agaacagtta agaaaatatt aaacaatgag
     7561 gaacttaatc taatataatt tttggctagt atgactggaa taaaaataag ctgaaaagag
     7621 gaggtgattt agtgaaggtt aatgggaagg aaattgaatt tgaaaaagat ttgactgtta
     7681 tagatttatt aaataaatat aatttaaaaa gtgatagagt tgtagttgaa gtgaatttag
     7741 agataattga agaatctaac tacaatacat atgtattaaa agacgaggac attgtagagc
     7801 ttattagctt tattggaggt ggttaaaata aaaatatttg taaatgaatt attaacagat
     7861 gttgaggaaa atacaactgc ttataaagtt agagatagct ataacaataa atgtgatgta
     7921 ttggtgttaa atggatatcc aattaaaaca gatatgaccc tttgtgaaaa tgataaatta
     7981 acacttatac aaaaaggagt taagccatca cttgatgaat tggaaagctt gttgattgct
     8041 agacatactc caaatataca taataaatta aaaaaaggga aagttgctat attgggattg
     8101 gggggacttg gttccaatat agctatctct ttagctagaa taggagtggg agaactttta
     8161 cttatagatt atgatgttgt agaaccatct aatttaaata gacagcagta ttttatagat
     8221 gatataggaa aactaaaaac tgatgccatg atagaaaata taaaaaaaat aaatccattt
     8281 ataaaattaa ataaacgaga tattttttta aacaagaaca atatggatac tattaaggat
     8341 tcagatttaa taattgaggc ctttgatgat gcttcatgta aagcacaggt atgtaattat
     8401 gtattaataa atttaaaaga taaatattta atagcatcat ctggtatggc aggttactat
     8461 gattcaaata taataactac taagaagata aaagacaggt tttatatatg tggggatttt
     8521 gtgaatgaag ctaaatttgg tgaagggctt atggctccaa gggttgccat ctgcgcaaat
     8581 catatggcaa atttggcaac gcaaatactt atagatatgt aaatattaaa attagatata
     8641 aaaaagattg aatattaaaa aaatttaacg tggagggaaa taatatggat aaattggtac
     8701 ttggtggaca tgaatttaat agcagattat tggttggaac aggtaaatat ggttcaaata
     8761 atatattgcc tgaggtaata aaagaaagtg gaagtgaaat aataacaatg gcacttagaa
     8821 gagtggattt agacaataaa caagaaaaca tattaacgta tattccaaaa gagatgacaa
     8881 tacttccaaa tacatcagga gctactaatg cagaagaagc tgtaagaata gctagaatat
     8941 ctagaaaaat gggatgtgga gattttataa aaatagaagt aatatcagat actagatatt
     9001 tacttcctga taatgaagaa actataaaag ctacaaagat acttgctgat gaggggttta
     9061 tagttcttcc atatatgaca ccagaccttt atgctggaag aagacttata gaagctaatg
     9121 cagcggcagt tatgccactt ggagctccaa ttggttcaaa tagaggtctt caaatgaaag
     9181 aaatgataag aataatgata gatgagttgg atataccaat aatagtagat gcaggtattg
     9241 gaaaaccatc tcaagctatg gaggctatgg aaatgggagc tgatgcagta cttgtcaaca
     9301 ctgctatagc ttcagcagga gaccctgtac aaatggctag agcatttaaa ttagctgtag
     9361 aaggtggaag agaagcatat attgcaaaaa caggaaatgt gtcagaattt gcaaatgcat
     9421 cttctccttt aacaggtttt ttaggcaatt tatagaaagt ggtgattggt gatgagtttt
     9481 tatgatgtaa tagaaaaata tagggatttt gattttgatg gatatttaaa taatgttact
     9541 gataatgatg ttttaagaag tttatcaaaa gacaagcttg aagattttga tatattaaat
     9601 ttgctttcta aaactgcagt aaaacattta gaagatatgg cacaaaaagc acataaatta
     9661 agtgttcaat actttggtaa aacagtatgt ttatatacac caatgtatat agcaaattat
     9721 tgtgttaatc aatgtgttta ttgtagctat aatataaaga gtggaataaa aagaaaaaaa
     9781 ttaactatgg atgaaattag agaagaggga gaagtaattt caaaagaagg tttcaaacat
     9841 ctacttgttt tgactggaga aagtagtttt cactcaagtg tagaatatat aggtgaggct
     9901 atagaaatat taagagagaa atttccctct ataggtatag aagtttatcc aatggaagta
     9961 gaagaatata agtacattgt agataaaggt gttgaaggat taacagtata tcaagaaact
    10021 tatgacgagg aaatatataa gagagtacat ataaaaggcc caaaatcaaa ttataaattt
    10081 agactagatg ctcctgaaag aggggcaaaa gcaggtatga ggactttatc aataggtgca
    10141 cttttagggc taaatgattt tagaaaagaa acttttttta caatattgca tgggaaatac
    10201 ttaaaaacga aatatcctca tattgagctt tcatattcaa caccaagaat gagacctttt
    10261 aaagggtgtt ttgaggaatt ggtagatata agtgatactg atttagttca agctatggta
    10321 tgtatgagat tatttgaccc tcatgcagct ataaatatat ccactagaga aaatttagaa
    10381 atgagaagtc atataatacc tttaggagta actaaattga gtgcaggtgt atctactgat
    10441 gtaggaggtc attctcaaga tgaacatgat actgctcaat ttaagataaa tgatgaaagt
    10501 actgttaaag atgtggagaa aatgctaaac tcaattggtt atcagcatgt gtttaaagat
    10561 tgggaaagat tttaaatgta tctgataaca aacagaaaat tatgtagtga ggaacgatat
    10621 ttagaagtta ttaaagagtc tatattgagt ggtgtagaaa atataataat aagagaaaag
    10681 gatttagaat atcaagagct aagaaaatta tatatgaaaa taaaaactaa aatcaattgt
    10741 atagattttc aagagcaaat atcagatgaa agtttgaaaa ctaatataaa tcaaaaagag
    10801 tttagaaata aattcaaggt taattttata ataaatagta acattgaatt ttttgaaaaa
    10861 gtggattgtc aaggtatcca tcttcctttt aaattattct tgaatttaat tgaaaataag
    10921 tataatttta atgaaaataa aatactagga ttatctttac ataaagtaga ggaagtagat
    10981 tatttagaaa aattaataag aaatcaaaat ataaaaatag attacataac attatcacat
    11041 atatatgaaa ctaaatgtaa agaaggatta aatccaaaag gaattgagct tttaaaagaa
    11101 gctaaaaaga ttactgatat taaaataatt gcacttgggg gaattttacc atctaatgtc
    11161 aaggaaacat taaaatactg tgatgatttt gctataatgt caactataat gaggtctaaa
    11221 gatattaaaa aaaccatatc aaattataat gaaaagttga attagtaaat atagtatatt
    11281 gaatttttaa cttaaataaa aataaaaaag ttatctttta aattttaaaa ggtagctttt
    11341 ttattttata tctttgctaa taaaaaattg taaaaaggga aaactttagt cagagagatt
    11401 aaacaatatc ctagaaagat ttaaaattta gtaaagtatg gaggacatta taatgactga
    11461 cagtaaaaag aaacgattta ttataccaca cacttttaca ataatatttt tactaatagt
    11521 cttgatggca atattgactt ggatagtacc aagtggtgaa tttagtagag aagatgtaga
    11581 aggtagaatg gtagttgtac cagggactta tcaaagtgta gaaagtaatc ctcaaggatt
    11641 tgcagatgtt tttaaagcac caatagaagg ctttatcgat tcagctgaag ttgtaggttt
    11701 tgttttaata gttggaggtg cttttggtat tgtaaatagg actggagcta tagaagcagg
    11761 aatagcttcg gttatctcta agtttaaggg aaaagaactt cttataattc caatatcaat
    11821 gattttattt ggacttggag gtacaacatt tgggatggca gaagagacat taccatttta
    11881 tatgatattt gttccactca tgacatcctt gggatatgat tctctaacag caatagcaat
    11941 tgtatttata ggagcagcag caggagcagc cgcttcaaca ataaatccat tttcagttgg
    12001 tattgcacag gcactagctc aacttgctcc tggttcagga gtaacttaca gagtagttat
    12061 ttttatagca tatatactaa taagtatagc atttgttatg tactatgcta agaaggtaaa
    12121 ggaaaatcct gaaagttcaa tagtatatga aatagataaa aataataaat caatacttaa
    12181 tcaaacttca acagaaataa aagaatttac ttttaaagaa gtatctgttc ttgctatatt
    12241 tggaataggg atggcaataa tgatttgggg tgttttaagt caaggatggt acattccaga
    12301 aatatcaatg tgttttgctg caataggtgt gctttctgga ttggttggta gattatctca
    12361 aattgaaatc tcagaaagtt ttatagatgg agcaaaagat ttagcatctg cagctcttgc
    12421 tatagctttg gctcgtggaa tcgtaataat tgctcaaaat ggatttataa tagatactat
    12481 attaaattcc gcagcagcct ttctaggtgg gcttccaaag gttatatttg tatatttatc
    12541 atttttcttg gaagctgcat tggcattttt aattccatca tcttcaggtc ttgcttcact
    12601 tacagtacct gtacttgctc ctcttggtga tttggttaat atatcggctc aagtgatagt
    12661 aaccgcttat caatttggtg tgggtcttac aaactttata actccaactt caggagtact
    12721 gatgggagca cttgcagtag cccatattcc attttcaaaa tgggtaagat ttataatgcc
    12781 tatcttgttg attttgacag ctgtatctct agtcttttta acaataggta tttatatagg
    12841 gttttaatta agtgtagttt tatttaagat atttagtaat ggtataaaat gataatatgt
    12901 tttcaataaa ttgccactaa tttaattagt ggtaatttgt tttttgtatt taaaaataaa
    12961 tgtatttact tgtattttga attaatatct acttagacat tgttttaatt gttatatttt
    13021 tatttaatta agcattgatt tatcattttt tatatttcat ataacaatgc aaaaaataat
    13081 ttttatatag taaatgacaa aagtttacaa taaatgtata ttttcttact cattccttaa
    13141 aattactcat ttagtttaat aactctcatt tcatatgtaa agttggtaaa aaaattaaaa
    13201 ttaaaagtaa aaaactaaat aaattaatgt taatttgaaa tatatatgaa ggtttttgga
    13261 gaatacttac ttatgatagg aaaaaatgtt aaaataatag tgtaatcaaa tataaaggac
    13321 tgaaataatt aacagttatt aaatattagg agtgtaaaaa tttatgggat taaaagaatt
    13381 tgattttatt gaagagtcta ttgatatgtt gaggacaatg tcaccaacat tagaaactat
    13441 atcagatgaa atagaagaat attttgaaaa tatcttggat gaaaagaatc aagaatatat
    13501 aaatgtaacc tctagaataa aatcagaatc aagtttaaga gaaaagataa ttagaaatag
    13561 atatttaaaa aaatatggag aagcaagtaa tcttattcat aatgtttctg acttaatagg
    13621 cctaagaatt gagtgtagat ttattgagga tgaaaacaaa atatatagac ttttgagaag
    13681 atattttaat aaaacagatg acaaaataaa ctattataat aaagagaata agaatattaa
    13741 attaaaacta tctgagaggc aacccaataa gcaaaaaaat ggatttgaaa tatataaaat
    13801 agatggagtt tttgcgtatt tagatagaga agtaaaattt gaacttcaaa ttaaatctct
    13861 tgtaaatgtg ttttggagtg aaatagaaca taaaataatt tataaaaaca atacatattt
    13921 acttgctgat aaatttataa aggatatgat ggactctata aagaataatc ttactatgat
    13981 agataatcag cttttaagta tttataaaaa ctttcattca gggaagtctt ttaatatgaa
    14041 agtaagtaaa aaagaaattg aaaaattatt tgcgaaacta gtctatgacg ctttttctga
    14101 aaagatgaat aaaagtatag gttttgttgt agattttaaa aaaccatgtg agactatatt
    14161 gaactattct tttaataaac aagatataag tgatgaagtt ttaggtaatt ttatgcttga
    14221 tgagtttgca agattaaatg aaattgtaaa caaggatata gattttaatg agcaaataga
    14281 atttgaaaga gaaccaacat ttgatgataa gttttgtaaa gacctaggaa atcattttag
    14341 aagtagatta aacacagaat ttccatggaa cctatttttt agaatattat ttgaaataga
    14401 gccatataac aataccaaag actttgaaaa ctttgtagag tttataaaag aaaatgtgct
    14461 actagaagaa aatagggagc aattattatg ccaatttgaa gaggatagta agcttataat
    14521 tgaagatata tatgattgta tatttaatag tataagtgag attgatagtg ttgaaatttt
    14581 atataactat aatcttgaaa aaataaactt tacatcaagt gaaatcataa attgtatatg
    14641 tagagaatat gagtgttatg gtgaatactt agaagaaaaa gaaaagctta tgaatacatt
    14701 taaagaaaag attattcaaa tctttgagta ataggtcaat gctgaagtgg cgctaatgcg
    14761 ccatttttca aatatatatg aattaagtaa aattattttg gaggaggcga tttgtataga
    14821 gaaaacaggt gtaattttgg cagggggaaa aaattctaga atgggtagag ataaagcctt
    14881 tttagagtta catgataagc tttttattga aattgcaata gaagcattta aaaactttga
    14941 tgagttgata ataatatcta acaatgaaga attatattct aaatacgata ttaaagttta
    15001 taatgatata gtaaaagatg ttggtccaat tggaggaata tatactgctc ttgaatatgc
    15061 aaagtatgat atagttacaa ttgcatgtga tatgccatat ctaaataatc aaatagtcga
    15121 aagaatagct aataaaatgg atgataaaag tgtgatttca gtgacaaatg gaaagttaca
    15181 accattgtgt tctggatata aaaagagtat aataaataaa gtatctttat gtataaggga
    15241 aaatgattta aagctcagaa gctttataga taaaatagat aaaagttata tatactttga
    15301 tgaagaagat ttatttttaa atgtaaacac tgttgatgag tataaaaaat taactaaagt
    15361 ttaattagct atggttgtaa aatagagtag gatttatatg gaggagagtt tatgaattta
    15421 cttaaatgcg attcttgggc agtaatatta aataatagcg ataaagaaag taaagcatat
    15481 aaaatattag atgaacttaa aaggaatatg tacaaagttg ttgctataga tgaagagaaa
    15541 aaacctattg aaggaataga tgtgtatgaa tgtttaaaag atgttccaca caatattgat
    15601 gttgtagcta ttattgataa acaatctaag atggatgtga ttttagaaga agtagagctt
    15661 ttggatatac aaaatatgtg gtttgaaaaa ggaagcttta gtgaaatgat gattaaaaag
    15721 actaaagatt taaaattaaa tatagaatat aatttaagtt tatatgatga gttgactaga
    15781 taaaattata tttcttaata ataaaaatat aattagttaa taaaacatat tattttatag
    15841 tatagattgg ttaataaaag tacagattgg ttaatgaaac attaaattac aatatttgtt
    15901 aaaaaaattg tgagttacat gatataatgt attaaatagg atactaagga gttgatatat
    15961 tatgttaata gtaactacag aaaaagtaga aggtaaaaag atatcaaagg ttttaggatt
    16021 agtgagagga agtacaataa gagcaaaaca tgttggaaaa gatataggag caagttttaa
    16081 gaatcttgtt ggaggagaac ttactggata taatgaaatg ctcactgaag caagacaaat
    16141 tgccataggt agaatggttg aagatgcaga agctaaaggt gcaaacgcag taatagcatt
    16201 tagactgtct tcagcttcag ttatgcaagg agcagcagaa atgcttgctt atggaacagc
    16261 agttgtttta gaagatgata atagtattct tgaaaaataa aatataaata gttaaaaaga
    16321 ggcatctttt aaaaatttta tggatgcctt tttttatttg aaaaaactaa tttgagaaga
    16381 aattttaact attatgacaa taaaattagg gtgatatta
//
LOCUS       DAGSHT010000065        16214 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGSHT010000065 DAGSHT010000000
VERSION     DAGSHT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.067"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..90)
                     /locus_tag="MJB72_001904"
     CDS             complement(<1..90)
                     /locus_tag="MJB72_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HBY8263012.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVD"
     gene            complement(71..1372)
                     /locus_tag="MJB72_001905"
     CDS             complement(71..1372)
                     /locus_tag="MJB72_001905"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBY8263013.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(1385..2299)
                     /gene="nadA"
                     /locus_tag="MJB72_001906"
     CDS             complement(1385..2299)
                     /gene="nadA"
                     /locus_tag="MJB72_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBY8263014.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            2717..4174
                     /locus_tag="MJB72_001907"
     CDS             2717..4174
                     /locus_tag="MJB72_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBY8263015.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            4397..4825
                     /locus_tag="MJB72_001908"
     CDS             4397..4825
                     /locus_tag="MJB72_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263016.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            4885..5193
                     /locus_tag="MJB72_001909"
     CDS             4885..5193
                     /locus_tag="MJB72_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBY8263017.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(5340..6362)
                     /locus_tag="MJB72_001910"
     CDS             complement(5340..6362)
                     /locus_tag="MJB72_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY8263018.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(6537..7043)
                     /locus_tag="MJB72_001911"
     CDS             complement(6537..7043)
                     /locus_tag="MJB72_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBY8263019.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(7103..7804)
                     /locus_tag="MJB72_001912"
     CDS             complement(7103..7804)
                     /locus_tag="MJB72_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263020.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(8022..9092)
                     /gene="buk"
                     /locus_tag="MJB72_001913"
     CDS             complement(8022..9092)
                     /gene="buk"
                     /locus_tag="MJB72_001913"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY8263021.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(9145..9720)
                     /locus_tag="MJB72_001914"
     CDS             complement(9145..9720)
                     /locus_tag="MJB72_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBY8263022.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(9722..11509)
                     /gene="iorA"
                     /locus_tag="MJB72_001915"
     CDS             complement(9722..11509)
                     /gene="iorA"
                     /locus_tag="MJB72_001915"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBY8263023.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(11570..12829)
                     /locus_tag="MJB72_001916"
     CDS             complement(11570..12829)
                     /locus_tag="MJB72_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY8263024.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(13091..15091)
                     /locus_tag="MJB72_001917"
     CDS             complement(13091..15091)
                     /locus_tag="MJB72_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8263025.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(15099..>16214)
                     /locus_tag="MJB72_001918"
     CDS             complement(15099..>16214)
                     /locus_tag="MJB72_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8263026.1"
                     /translation="VSIKIDLIWAYLKEDMNKEAEVILLKTIKLMKVVEGVYKDYLYS
                     FIFLYSAEIELKKDNCSFEKVNTYLILSKNIYKSFGDVFIYNSKLDYIYKLYGDLYIK
                     FGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKDFKLAIEYYKSMDWY
                     SDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDILKDGLTKLYNRRYLE
                     QQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVTDIIRSVFIDITEHI
                     IRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEHIYSKIKNYITISAG
                     VSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII"
BASE COUNT         5223 a         2939 c         1740 g         6312 t
ORIGIN      
        1 atccacaata gaattagttg ttatatcttc acttggaaca tcttctatca aagcatcctg
       61 tatcattcta tcaataatca aataattcat cgccaaaatc ctcacttgtt ctttcaattt
      121 cttttatgac taatatttta ttttcttttt ttacctgttc tatatctata tccatatctg
      181 gtacatctat aaacttaact tttacattat ctataactga gtttatattt ttagctgctc
      241 tttttgaaaa tacaagccct tcaagtaatg aattacttgc caatctattg gctccatgaa
      301 cacctgtaca actaatttca ccaactgcat ataaattttc catagatgtt tttgaatcca
      361 aatctacatg tatcccgccc ataaaataat gttgtgcagg agataccttt atacattctt
      421 tcgttatgtc tgttcctcta gctaaacatt cattataaat aaaagaaaat ctattaatta
      481 aatattcact atctaaatgt gttgcatcta aatacacata aggtaattta tctttttcca
      541 tttgctcaaa aatagctttt gaaactacat ctctaggaag aagttcatcc acaaatcttt
      601 ctttattttt atttaaaagt ttagctccct ctcctcttaa tgattcagat ataagcattc
      661 tcctaccttc attattttct tcataaaagg ctgtaggatg tatttgaata tattctaaat
      721 tttctatttt tatattattt cttaaagcta ttgcaattcc atcacctgta agatgtcttt
      781 gattagtaga atttttaaaa agcccaccaa ttccacctgt agctaaaact actatttttg
      841 aaaaaacatg aatctctttt ttagatttaa aaattctagc tcctatacat ttattgccat
      901 cttttaatat atctaaaaga taggcatctt ctataagagt tatattttct ctagttttag
      961 cttcttttaa taaagtttta aaaacaactt ctccagtatt atccttacta tgaactattc
     1021 gatttacact atgtgctcct tctcttgtgt aatctatctc tccattttct ttgtctaaag
     1081 gcataccata ttctactatc tgccctacat tttctataga ttcatctgca agtacttgaa
     1141 ctgcttcaac tctattttta tactgcccag ccttcattgt atcttctaca aaggattcta
     1201 tatcatcaat atttctagca gttgaaattc caccttgtgc aagatatgta ttgttgtttt
     1261 caattgttga tttcgatact actagcacat ttaaactttt atctaaattt aatgcacagt
     1321 ataatcctga aactccactt ccaactatta aaacatcttg ttctaaattc ataagtctat
     1381 ccctctatcc ttctgctaat ttgtgcatgt taagcagtga atttaaagcc ttttccatga
     1441 tttcatcttc tacaataact tcattttcca tatttttaag tgtactatat aaattttcta
     1501 atgttgtctt tttcatatct gtacatagta tttttccacc aggaatataa aagtttttat
     1561 taggattatt tttctttaat tcatgtaata taccttcttc agttgctatt ataaagtctt
     1621 tgtttttact attagtagca tattttatta ttcctgatgt actacctaca taatctccta
     1681 aatctcttat ttctttttta cattctggat gtactaatac ttctgcattt ttatacttat
     1741 cttttaactc aattatttct tcagctctta tattgttatg gtatttacaa tatccgtccc
     1801 aaaatataaa gtttttatct ggaaattgtt ctgcaatata tcctcctaaa tttttatctg
     1861 gaaggaatat aatttcttta ttatctatgt tttctagtat tttaattgca ctagaagatg
     1921 taacagaaac atcacaatgt gccttaactt tagctgtaga gtttatataa catacaacca
     1981 aagcatttgg atactgttgt tttaatttta tcacgccttc ttcatctacc atatctgcca
     2041 tagcacaccc agcgttactt actggcatca atacagtttt ttcaggtgat aatatcttag
     2101 cactttctcc cataaacctt actccacaaa atacaaccac ttcttcttta caatctctag
     2161 caatctcact taaataatat gagtcaccaa ctgcatctgc taattcttgt atttctggtg
     2221 gttgataaaa atgggcaagt attattgcat ttttttcttt ctttagctct ttgatttgat
     2281 atgttaaatc tttatccata attgcaccgc tttctatatg tactagtgta tatacacctg
     2341 taaactcatt atatcacttg actattcttt cttcaagaat tttaaacctc ttaatattgt
     2401 taagatatag acatttatat tctcaattga ttttcctttt ttctccatct atttaaacta
     2461 atttttatat tttttaattt ttttgtaaaa tattatcaga attttgtcta tattctttat
     2521 tgctataaca ttgaaaatat taccaaatac ctgtatacta agtacatggt tatgtatatg
     2581 tatttatttc tgaaattata ttagttgtaa tactttatag aatttgtagg ttgtccagaa
     2641 ttaaattcaa tataaatcta cacatttaga taatatgtat acaaagacca aaaaatttta
     2701 taaatggggt gatattatga caacattctt aatcggcttg gctatattac taataggagg
     2761 tgctttatac ggtgcttatt gtgaaaaagt atttggccca gatgacagaa aaacacctgc
     2821 acttgctcaa agtgatggag tagactatgt cccaatgaaa aaatggaaaa atagtctaat
     2881 tgagttgctt aatatagctg gtacaggacc aattctagga cctatccaag gtatattgtt
     2941 tggtccaata gcattcatac taatacctat tggctgtgta tttggaggag ctttgcatga
     3001 ctatatgagt ggaatgatta gtataagaga aaaaggagca caaatgcctt ctcttatcag
     3061 tagattttta ggtaataaag tatttcaagt ctataatata ttcctttgtc ttttaatgct
     3121 tttagttggt gcagttttta tttatacacc tggggattta gttgttactc aaattttaaa
     3181 tatgaaatcg actataaata atccagttgt ctggatagtt tatggactta tctttctata
     3241 ttacttgtgt gcaacactat ttccaattga taaaatcata ggaaaggtat atccaatatt
     3301 tggagctatt ctacttttat ctgctgctgg tgttggaatt ggaatcttta cacaaggtta
     3361 tgatttagct aatttaagtc ttgcaaattg gaaaggtatt catcctgatg gtattcctct
     3421 tattcctaca ttctttgtta cagttgcttg tggtattgtt tctggattcc actcaactca
     3481 agctactctt atagcaagat cagtttcaaa tgaaaaagaa ggaaaaacta ctttttataa
     3541 tatgatgatt ttagaaggtt taattgcaat gatttgggct gcagctgcta tgggtattta
     3601 caataaagga atacctaagg aattagtagg ttctcctgat gtaataggtt tggttgcaag
     3661 agatttactt ggttctattg gtggtatcat agctataata ggtgtaattg ttttaccaat
     3721 aacttctgga gatacagctt taagatcttt aagattaatg cttgcagatt actttcatta
     3781 tgaccaaaag gaaaaaaaac atcgtgttat attgtcaatc tgtatattta ttcctgtcat
     3841 agccatcctt atatttgcaa agcttagtgc ttctggattt aatatattgt ggagatactt
     3901 ctcttggagt aatcaaacta ttgccatatt tgcatttgca atgattacag tttaccttat
     3961 aataaaagaa aagaattata ttataagctt aattcctggt atgttttatt cctttgttat
     4021 attcagttat atatttaatg ctcaaattgg atttaactta aacataaata tatcatatgt
     4081 tttagctgcc atatttactg tattatatgc tattttaact gtacgctctg gtagaaagtt
     4141 aaaaagcaaa gcagatacta agttggctga ctaaatctaa ataaaataat acttagaata
     4201 aattttatta aaaaatgata aaaaagtagg caatctcaag ttagatttgc ctctttttta
     4261 tttaatacag taataaatac ttaagtattt actccttatt ggaatattta tcctaaatct
     4321 ctaatataaa taaataaaag ttttaaaata atctattcgt ttatatactt aaaataaatt
     4381 ttttggagga atacttatga ataataaatt acatgaactt gaaaaaaatc taccagaaac
     4441 atcttgttct ttttgcacac atctttcatt taaggggcct agcctagact atagatatga
     4501 tattagatgt gttatttcag atacaaagcc cgattttaga ggatgttgtg aatattttga
     4561 accagaatat acagaattaa atacaggaga tttagacaat ctatacataa actttcttga
     4621 gacatgcctc aaggtaaatt ataatgatta catcaattct atgtattgga aattatttaa
     4681 agaaagaacc ctactagaaa acaattttaa atgttctata tgtggatcaa ctgaaaatgt
     4741 tgatgtgtat catgttaata aaaatttagg aagggaaact tccaaagatg tagttgttaa
     4801 atgtgataaa tgtaattgta aataagtgta taaaatttag ataacttcac ataataagtt
     4861 taaataaaac ttaggaggtt atttatgaaa aataataatt taaattgtgc tgctactaat
     4921 tgtgcttata atactagtgg atattgttat gctggtagta taaaagtaga tggaatgcaa
     4981 gcaactacta cagaaaatac ttattgtgct tcttttgaag acaaatatac atctggtata
     5041 actagtcgtt ctaatgaaac taaccaagtt gatacagata acattcattg tgaagctgta
     5101 aaatgtaaat acaataaaaa tgaactttgc aaagctgaaa aagttcatat aaatgaaaga
     5161 aatgcaagtt gtgaaacttt tgaaatgaaa taaatttctt tttatttagt tttatagtat
     5221 ttatacaaag caaaattgaa cctatcaaat agatgtcttt attaatgatg tctatttata
     5281 ggttcttttt taaaacaatt tataatttat atataaagat aaattattat tttaatatat
     5341 tacctagaag taaatttaac acccagaata tccataagga acataaatat tggaactcca
     5401 attagtaatc cccatacacc taaataatgt tctcctacaa gtaaaataat aaaaacaaaa
     5461 caaactggaa gtttagttct attcgacatc aacttagggt ttaatatgta tgtttctact
     5521 gcatgcacta tacaaatcat aagtaaaact ccaaaaacct ttgttattcc tcctgtatta
     5581 aatgctatta cagttaatgg aatcaaagac actataactc ctgctactgg aataagaccc
     5641 aacacaaaaa tcataaatcc taaagcccat atttgaggga atcccatgaa acctaagaaa
     5701 atcatagaaa caacagaatt tataaaggca atcataactt gtactttcat tacttcacca
     5761 aaatttttaa caaagctctt tccaaaaaat acaagcgaat tataaataaa agaaatctta
     5821 ctatcagaaa gtttatctcc aaaattttta atttcatttt tttcaagtaa aaggagtaaa
     5881 ctcagtaaaa atgccaataa tatatttact ccaaaacttc caacttttgt tgctgttgat
     5941 gctataagta gccctgcttg tgaaatatat ttattaaaat ccagctttga gacaatagga
     6001 tacaattttg gtcctaaaga ttgagcaaat tgatttacat caaaatcact aaaaacccta
     6061 ctcaactctg taagttgaac tataattatt ggtacaactg catagctaat caatactaac
     6121 aatatagcaa atagtgcata taacactata attattaatg aatctggaat atttaaagaa
     6181 gaaaacttct taattcgttt tcttgtggct acaagcaagt tatagaaaat aaaagtcata
     6241 ataaatgtta aaagcaccat atttaacata taccaaaatg taattagaat aattactacg
     6301 acagatatag aaacaatttt atattttgaa ctgtcacgtg ccaatccttt gttatcttcc
     6361 atagtttaaa tcctccccaa atttttatga ctttataatg actgcccttt ttattttaat
     6421 ctcaaaaaac aagtagaaaa atactaaaaa taacttatta attttcataa aacaatatta
     6481 aaatttagag aagatacatt ttaaataatc ttacttatac tgaaatctgc tctacatcat
     6541 atatttaatt tattattatc tatctcatat agcaattcgt atatctctgg atattcaaaa
     6601 aacaaaccct cttttagtaa ttgtcttatt tcatctgtgc ttacccactt tatatcactt
     6661 acttcttcct cctgtaaaat agctttggat atatcatatt cttttttaac taagtataca
     6721 tcccaaagag tatcttcatg aatcatactt ctaaaaactt tcatctcatc tttagttata
     6781 tctatcccta tttcttcctt tgcttcgcga attgctcctt ctaaactgtc ttccccagaa
     6841 acaatacatc cagttgtcat tccccacata tttggcaatg tctttttact tttagacctt
     6901 ttctgaatta aaatttgaga gttactattt aaaatccaca cttcaactgc caaatggtaa
     6961 taaccttctt caatagaatt acctcttttt ataacatttc ctgttttaat tccatcagca
     7021 ttatataaat cccataattc catttcaaaa tctccttgac tttactataa attagataaa
     7081 ctacaaatat attttagcat aattaattct gctcattttt tattacaata cttgatttac
     7141 atttatgttc attgcttttc ttttccttcc attctcttat atcatctata ataaatctat
     7201 gtttcaacaa ttttgtgtca aatttaaata ttctaaacac tatttgtaat acatatccta
     7261 aacctaaagc acttacaagc gtaccaatac ctacagaacc tcctaataac cacccaacaa
     7321 ctaatgctag tatttcaaga attgttctaa caagacgtac agatttatta gtttttttct
     7381 gaagagcaat cataagccca tctctcggtc cacttcctaa acaaacacct aagtatagaa
     7441 aacttgctat agctgccaaa ataattccta taacaaccat aaacacacct atataagtgt
     7501 tgcttgcatg aggtatcact ccagaaaata ttactaaatc aataaaaatt cctataaatg
     7561 tcatgttgag taaagtaccc catcctatat tttcaccaaa aactgcatcc aatataacaa
     7621 ttacaactcc taccattata gaagcctgac ctatagttat gcctagcttt atagataaac
     7681 cttggtgaag tacatcccaa ggcataagac ctacatgtgc attaatcata agcacagaag
     7741 aaattgcaaa tataaaaaaa cctgaaaata atcttatcat actttttaaa cttatttttc
     7801 ccatcaaaaa tcactcctca taataataat aacaataaat accaataaaa tatatatata
     7861 aatacgaaat tttacagaat tatttattag gttaagataa aattttaatt ataaaaagtg
     7921 gcaatccaaa ttttatacat tttcacctat actctaagct ttcgtgtata aaaaacggat
     7981 ataccacttt tttagtatat aaacattttt agtttttctt attaattttc actataaatt
     8041 ctagcttctt cttctccatt tagtactctt aaagttccaa aagcaagtga ttccaactca
     8101 ttttctcctg ccataatttc tactggtgct ataaactcaa ctttcttttt aaccattgaa
     8161 gtaaacattt cagaatatgc aattccacct gttataatta tagcatctac ctttccatct
     8221 actacagttg ctaactctcc aatacctttt gctaactgat aagccattgc ttcataaata
     8281 atttttgctt cttcatttcc ttctgctatc attttttcaa cttctctagc atctacagta
     8341 tttaggtatg aaactatacc acctttacct cttatctttt tagtcatttc tctttctgaa
     8401 tattgacctg aataacaggc agccactaat tttgtagcag gtactcttcc tgacctttct
     8461 ggagaaaatg gtccttcatc atcagatagc atatcaacca ttcttccttt ttcatgaaga
     8521 taaatactta ctcctccacc aatgtgagct actattaagt ttaagctctt ataatctttt
     8581 cctttatcct tagcatattt taaagccatt gctctagtat ttaatgcatg ccctgcactt
     8641 gatctatcca ttccacaaag tccagatatt ctcgctacat ctataagctc atctactgca
     8701 acagaatcat aaatatatga gttgattcca agaggttctg atattgcatg tgccacaaca
     8761 gcacctaaat tggaagcgtg ttcaagtact ggtctatgtc ttagtacatc aatcatttct
     8821 tcatttacta aatatgctcc tgattttact ggtggtaaaa ctccacctct tcctactatt
     8881 gcactaagag attttaagtc tatactattt tctttaagaa tattcaaaac tgcttcctta
     8941 cgcatttcaa actggtcttg tatagtatta tattttgcaa tctcttcagc tggatggtct
     9001 atcgtcttca caagaatttg ttcttctcca tcatatactg ctatttttgt agaagtagaa
     9061 cctggattta tggctaatat tctgtaagtc ataattttct tcctttcacc ttaatttcta
     9121 tagtctattt atatctatta taaactattt gctaaccatt tccatacctt catggaaagc
     9181 ttttatgttt aattcaacaa attttggttt aacattttca cttataattt tttcccaatc
     9241 aatagactct aattgcatag atttaactag acatcctaat aaaataacat tcattatttt
     9301 tgaatttcct agttcttcag ctttatcagc tgcatttata acttttgcat ttaatctact
     9361 taactctgca tctatatcac tttcttgata ttcagctcct ccgattaaaa cagtcataga
     9421 atctatccta tgattattca ctaaagcttt tccttctggt tttaaataat tgaaccatct
     9481 taaagcttcc attttttcaa aagatactaa tatatctgct ccaccaattt ctataactgg
     9541 agaatgaaca caatctccat atctaacttg tgaggaaact gaacctcctc tttgactcat
     9601 tccatgtatt tcactcatct ttacatcata tccagcttcc atcaatccca tagttaataa
     9661 tttactagca agtatagttc cttgacctcc tacacctacc aaaagtatac tcttagtcat
     9721 attatttttc ctccttaact attgcttgtt ttggacaaac ttgtgcacat actccacatc
     9781 ctacacactg atttctatct atatttgata acttattctc tttatcaaat gatagagctg
     9841 gacatccagt tttcaaacat aatttacatc ctatacattt atcatggtca actttacact
     9901 tagtcttaaa tggattatta aattcttcta tatcttcttt agagaatttt ttaagtacac
     9961 atggccatct tgttattatt actgatggtt catcttctat agctaaagcc caatctagtg
    10021 cttcatttac ttcttttaaa ttattagggt ttataactct tacatgttct attccacaag
    10081 ctttaactaa tccttctata tcaacttctt tagttttagc accttgtaaa gtatatcctg
    10141 aacctgggtt ttcttggtga cctgtcattc cagttattct attatctaat ataaccgaaa
    10201 tagaattacc tctattgtaa acaacatcta aaagtccatt tattcctgta tggaagaatg
    10261 ttgagtctcc aagtacacct accaatcttt tttcattatc atccttcata ttaagaactt
    10321 tttgagctcc atgtgcagtt ccaaaagcag aacccataca aacaacataa tctattccat
    10381 tatatggagg agcaaatcca agagtataac aacctatatc tccaccaact attaaatttt
    10441 ttctctttcc aagttcatag aagaatcctc tatgtggaca tcctgcacag aaacttggtg
    10501 gtcttggtgt tactaattca gatttgtatt ctatagtatc atttgttttt ccaaatatag
    10561 cttttcttaa aacatctggt gtcatttctc catatggtgg tattacatct tttccaatac
    10621 aatctattcc atatgctttt atttgttctt ctataaatgg gtcattttct tctataacat
    10681 atattttatc aactttttct gcaaattctt ttattttttc ataaggtaat ggatttgtaa
    10741 atcctagttt catatatgat gcattttttc caaaaacttc tttggcaaaa ttacaacaca
    10801 taccagatgc tataacacct atttttgtat catttatttc atagtagttt aaatcagttt
    10861 catttgagaa ttttttaaga atttccattc tttcttcaac aacgcccctt cttatttgag
    10921 catttgctgg aacagttacc attttcttag catttttaac atactcttta atttcaactt
    10981 cttctctatc atagcactcc actaatcctt ttgaatgaca aagtcttgtt gtaactctat
    11041 ataaaactgg tgtatcgtat ttttcactaa cttcaaaagc ttcttttatc atatctttag
    11101 cttcttgact agttgatggt tcaaataaag gtatctttgc aaattttgca tacatcctat
    11161 tgtcttgctc attttgagat gaatgcattc ccggctcatc tgctgtaata agtaccatac
    11221 caccatttac ccctgtatat gcataagtaa atattggatc agctgcaaca ttcaaaccaa
    11281 catgtttcat agaagccatt gttcttgctc ctgcaattga accccctata gcagcctcaa
    11341 gtgctacctt ttcgtttgga gcccactctg caactattgc atctttatat gttgctatat
    11401 tttctaatat ttctgtactt ggagtccctg gatatgcaga tgcatattta acaccagcct
    11461 cataggctcc acgtgcaatt gcttcattac ctgtcatcaa ttgtttcata taaaattccc
    11521 tccatctaca attattaaat tcatagtcca cccaatttgt tcaatgaaat taattattta
    11581 tttttacttc taaactttca agtgcttctt ttataatact tggtgctttc ttacatttat
    11641 cttcagaaac tgcacaaact gcaaatctta agcccatttt taatggaact gcaaataaat
    11701 tatgtttagt caattcttca catacttctt ttggattatc acatggtata cttacaaaga
    11761 aacctgctat atatggaaga atttctaatc ctactctttc agcttctttt acaaatacat
    11821 ctgctcttct agtaagcatg tttttgtata tttctttttc atcctcatat tctttgaatt
    11881 ttttagggtc atttacaatg ttagataata ctgccatagc acttctatta caatttgacc
    11941 aatttgctct acatgaatga acacatgagt aatggaactc ttcagctaca tcttcacttg
    12001 aagatataca aattatagct cccattctca taccataagc tgtaaatcct tttgacatac
    12061 tgaatccaac caatacgaga acgttttcag gcaaattaga aaatttctta aaaaactttc
    12121 tacatacatc atcatcacct gcaaagtcaa tatatgctga atctacgaaa aatattatct
    12181 ttttgtcttt atcttttgcg acttcttttg ataagtctaa tattttatcc cactcatcat
    12241 ctgcaacact atatccagtt ggattatgcg ctggtgtgtt tataattgta aagactctac
    12301 cttgtttttc tgctatattt acaaattttt ctttaaacga ctcaaaatta aaccttctat
    12361 tttcatcaaa aagttggtaa ttaactactt ttctattagc ctcttcagct atacttacat
    12421 atggactcca gaaccaatca gatgttaata cctcatctcc ttcattagta tagttccata
    12481 cagctaattt tattgcacca cttcctcctg gtgaagctaa tactcttata tgtccttctg
    12541 gtaagtaatc tctgaaaaat acttttttaa ctgcttctaa gtaatctggt tgaccttcaa
    12601 gagctgcata tgcacctatc tcacagttat ctaatgcttt atattcttca tatacagttt
    12661 tcattgttat caattttcct gaatcatcca ttagagcgcc tattgttgca tttattacat
    12721 tatccatacc aaaagccttc tctgctgctt gagctctgcc agatatgcta aatataacat
    12781 cattttcttt tggccacatt gcatgcttac caaccatact ttgactcata ccatcacccc
    12841 taaaattgta tttactctta cattatatat taagcaatta ctatgccaat aaatcccact
    12901 ttatgtaaaa ttgattttgt ttagaattat tatttttttc attattaaat taagtgtttt
    12961 aactattttt cttataaata tatatgcctg tctaaaaaaa taaaaatttg caaaaaagta
    13021 atgttaattt ttcttaacac ttgttttaaa aaattaacat tacttcaaaa taatttatat
    13081 gtaattatat ttaaatattt ttattgtatt tcttccataa agatgtcctg cttattccaa
    13141 gaacttgtgc aacttcattt ttagaaagtc cctgagcaaa aagcatactt attatttttt
    13201 cttcaacata cttatttact tcttttatat ctatacttat cttaccatca tttatcttac
    13261 ttatatttat agattcatct tggacttcta cacttgctgt cgtttcagaa ttaaatttgt
    13321 ctacttcaga gttaggaagt aattttttag ttttaaaatc tctatcttgt ctcaatttaa
    13381 ttttacctgt cacaacatat ttttgagcta ttgtctttaa ctctcttaca tttccaggcc
    13441 agctataatt atatagctta tccatttcat ctttagtcaa aacaaaattt tcatctatac
    13501 tgtttattcc attgagacca tcatccttta atacttcatt tacaaaattg ttaaacaaag
    13561 gtattatatc ttctcttctg tctcttaaag gaggtatatt tatctctaaa ctactcaatc
    13621 tataaaataa gtctgctcta aatgtaccca taactatttt ttcggttaaa gattcattcg
    13681 ttgctgctat aatccttata tccagaggta ttatataatc agaaccaatt ctcattattt
    13741 gacgttcttc tattactctt aatagttttg tttgaatatt aaaggataga ctatttattt
    13801 catctaaaaa aagagtccct ccatgagcaa gttcaaatag tcctcttttt ccaccctttc
    13861 tagcaccagt aaatgcacct tcttcatagc caaaaagttc actttcaagt aaattttctg
    13921 ctatagtagc acaatttata gcaacaaaag gcctatcttt tcttttactt atgttgtgta
    13981 tactatgagc tataatttct ttaccagacc cactttctcc ataaagtaaa gttgtataat
    14041 cactttttcc aattttttta gcttcttcaa taaattcttt agttaattta tctttaaata
    14101 aaaaatcatc aaaagtatat ctagcataga gacctttttg attcaagtca aatcttattt
    14161 ttctttctaa gttttgtagc ttggttatat cttgtattat tcctaaaact ccataagtac
    14221 tattatcaac ttttatcaat gttgtcctag tattaacaat tagattattt atatttctta
    14281 tctttctatc ttctacgtcc tctttttcat gtaaacaatc taacatccac tccattttag
    14341 gaaatacatc taagatgtat ttattaagtg cacattttct ctctacttta agcatgtttt
    14401 ttgcactttc attgtacaaa attatactgc cattactatc tatagcaatt actccatctt
    14461 ttataccatc aagtatattt cgtaacactt cattattaaa cttttcttca cctaatgtat
    14521 ctaataattt tttacaatat tctacagctt ctcttataga ttcatcacta gcagttgcaa
    14581 atacactatc aattccatat tgccttgcaa aagaacatgc caaacctcct cctacgatta
    14641 caacttcatc tttttggtct atatatttta caactttact tcttatctca tacttagatt
    14701 caaaccattc ttcagttatt tcagtactta tgacatttct ccagccaaca tagtcaaaag
    14761 aaacttcatt gtctccaaga atcaatacta cttttttaga atatttcttt gctatttcta
    14821 ttgctcttaa aaggtcagtt gacttcatat tcattggtat aacaggtaca tttactgtat
    14881 ctattactaa gctataacct ccacctctac caattatagc ttgggcacct ttttcaacaa
    14941 gtatacgacc ttgattttcc attaagtcta ggttaagtat gtcgattatt atagttccat
    15001 tttctacatc ttctctatat aattcttcta ttctttcttt taattcaata tcagaggcta
    15061 taattcctat tttttttcgc aacaaaatca cctcaatttt aaattataat gtattgattt
    15121 ctacctgctt tttttgattt atataggttc ttatctgcat cttcaattaa tatttcaaca
    15181 tcttttctac tacgacacct ttttatactt actccagcac ttatagttat atagtttttt
    15241 attttagaat atatatgttc aataccctca aattcaagaa gtttgaaaac tcttttaata
    15301 tataattcta tattatcaat ctcttcaaca ccgattgatt ctatagaatc tactaatatt
    15361 aaaatttctt ctcccccata tctgattatg tgttcggtaa tatctataaa tacacttctt
    15421 ataatatcag ttaccttaac tataacttta tcccctgcta gatgaccata attatcatta
    15481 taacttttaa aacaatctat atcgaccagt attgcgacca tatttttgtc tctggttcca
    15541 ttaaataaat ccaactgctg ttccaaatat cttctattat atagtttagt aagtccatct
    15601 tttaaaatat cattttctaa tttcttatta tcatccacta gtaatcttaa actctcttgt
    15661 ttttgtctga gttcatattg tttgtgaatt gcttgagatg ttttatagga attatttttt
    15721 tcccacctat cagaatacca atccatactt ttataatatt ctattgccag tttaaaatct
    15781 ttttttagct catatccatt tgctattagc ccataaaata tagatatttt tctaagattc
    15841 aatgaactct ttttaactaa atttaaacct tttatataac aacttatacc ttcatctata
    15901 ttaccaaatt ttatatataa atctccatac aacttatata tgtaatctag tttagaatta
    15961 tatataaaaa catctccaaa acttttatat atattttttg ataaaattaa ataggtgttt
    16021 accttttcaa aactacaatt atctttcttt aactcaattt cagctgaata taaaaatata
    16081 aatgaatata aataatcctt atatacacct tcaacaactt tcattaattt tatagtcttt
    16141 aataaaataa cttctgcttc tttattcata tcttctttta aatatgccca tataaggtct
    16201 atttttatag acac
//
LOCUS       DAGSHT010000066        16204 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGSHT010000066 DAGSHT010000000
VERSION     DAGSHT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.068"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1328
                     /locus_tag="MJB72_001919"
     CDS             <1..1328
                     /locus_tag="MJB72_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY8263027.1"
                     /translation="IYDKLNFKIKLQRIAYTDNLTGANTIDKFVIDANKILCKNTQVK
                     YALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEMFARISADNFIIIMKY
                     IEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPEDRDINSIIDRANIPHK
                     MAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYLQPKIELNTGEIQGS
                     EALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIYLRKWIDAGINPVTVS
                     VNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDNFDILIDIMNNLKKIG
                     FLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKIIVTKVIEMAKELGM
                     KVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIVSNLIRG"
     gene            1504..1872
                     /locus_tag="MJB72_001920"
     CDS             1504..1872
                     /locus_tag="MJB72_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8263028.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1874..2599
                     /locus_tag="MJB72_001921"
     CDS             1874..2599
                     /locus_tag="MJB72_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263029.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2593..3357
                     /locus_tag="MJB72_001922"
     CDS             2593..3357
                     /locus_tag="MJB72_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263030.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3579..4829
                     /locus_tag="MJB72_001923"
     CDS             3579..4829
                     /locus_tag="MJB72_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8263031.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            5020..5904
                     /locus_tag="MJB72_001924"
     CDS             5020..5904
                     /locus_tag="MJB72_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBY8263032.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6266..6883
                     /locus_tag="MJB72_001925"
     CDS             6266..6883
                     /locus_tag="MJB72_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBY8263033.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6991..9522
                     /locus_tag="MJB72_001926"
     CDS             6991..9522
                     /locus_tag="MJB72_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY8263034.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            9956..10657
                     /locus_tag="MJB72_001927"
     CDS             9956..10657
                     /locus_tag="MJB72_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBY8263035.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10707..11780
                     /locus_tag="MJB72_001928"
     CDS             10707..11780
                     /locus_tag="MJB72_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8263036.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIV"
     gene            12050..13150
                     /gene="vanG"
                     /locus_tag="MJB72_001929"
     CDS             12050..13150
                     /gene="vanG"
                     /locus_tag="MJB72_001929"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBY8263037.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPILHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13147..13953
                     /locus_tag="MJB72_001930"
     CDS             13147..13953
                     /locus_tag="MJB72_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY8263038.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            13971..16109
                     /gene="vanT"
                     /locus_tag="MJB72_001931"
     CDS             13971..16109
                     /gene="vanT"
                     /locus_tag="MJB72_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBY8263039.1"
                     /translation="MMRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         6178 a         1614 c         2888 g         5524 t
ORIGIN      
        1 taatatatga taaattaaat tttaaaatta aattgcaaag gatagcatat acagataatc
       61 ttacaggagc aaatactata gataaatttg taattgatgc taacaaaata ctatgtaaaa
      121 atactcaagt aaagtatgct ttattatata tagatattga taaatttaaa tatataaatg
      181 atttatttgg atatgaagtt gggaatgaaa ttttgcgtaa tttaacaaag attattaaga
      241 gtaatatatt tgaagaggaa atgtttgcaa gaatatctgc ggataatttt attataatta
      301 tgaaatatat agaagaagaa gatataacaa agaggttaaa aactatattt gaagaattag
      361 acttgtttaa taataatcaa gaagaaaaat ataagttagt tttaagttgt ggaatctatt
      421 ttatacttcc agaagataga gatatcaact caatcataga tagggctaat atacctcata
      481 aaatggcaaa gggaggacat aaaagttcat atgcatttta tgataataag attcatgacc
      541 aagagataaa agaaaaagag atggaaaata caatgttttc aagtctagaa aataaagaat
      601 ttattatata cttacaacct aagatagaac ttaatacagg agagatacaa ggttcagaag
      661 cacttgttag gtggaaacgt cctgataaag ggctaatacc tccaaatgaa tttatcccat
      721 tttttgaaag aaacggtttt gttataaatt tagatttata tgtgcttgaa gaagtatgta
      781 tataccttag aaaatggatt gatgcaggaa taaatccagt tactgtttca gttaatgttt
      841 caaggataca tttatattgc aataatttta tagaaacata caaaaatata attgataaat
      901 acaatatacc tgcaaagtat atagaacttg aattaacgga gagtatcata tttgataatt
      961 ttgatatttt aattgatata atgaataatt taaaaaaaat aggattttta atatctatgg
     1021 atgattttgg ttcagggtac tcatcattaa atatgttaaa agaaattcct atggatatct
     1081 taaaacttga ccaaaaattt attatggaaa cttataatag taaaagaagc aaaataattg
     1141 ttactaaagt aatagaaatg gcaaaagagt tgggtatgaa agtcatatca gaaggagttg
     1201 agacagaaga acagtttaaa cttttaaaag aagtaaagtg tgatatggca caaggatatt
     1261 tgtttggaaa gccaatgcca attgaagaat ttgaacattt aattgtatcc aacttgataa
     1321 gagggtaatt aatctgtaat ataaagaatt taataaattg tgttaaaaat ggactctaat
     1381 gtagtgttca ttttttttgt aaaatttttt atacttttac taaaaagtaa ttgacatata
     1441 ctttgtatta ttatattatt gtattaacaa cttaatacaa taatataata aggaggcatg
     1501 aacatggaat gggaacttga taataacaaa cctatatata tacaattagt agaacattta
     1561 aagttaaaaa taatttcagg ggaaattaaa ataggatcta aattagaaac agtgagagca
     1621 ctagcagaag atgcggaagt taatccaaat acaatgcaaa aggctctaac agaacttgaa
     1681 agacaaggtt tagtttatag tcagagaact aaaggaagat ttgtaacaga tgataaggaa
     1741 aaaataaaag ctatgaaaga ggaaatagct aatgtggaga taaatacatt aaaagtaacc
     1801 ttagaaaagc ttggatatga tagagatgaa atgctaaaac ttattacaga aaatctgaaa
     1861 ggagaattat aaaatggata aaaatataag caatgtttct caagatggaa tagttgaatt
     1921 taaaggtgta aacaagagtt atggaactaa gaatgtttta aaaaatattg atttaaatat
     1981 accaaaggga aaaatagtcg ggctgttagg acctaatgga agtggcaaaa gtacaatgat
     2041 taagctaatg aatggattat tacagcctga taatggtgaa attatgataa atggaatgaa
     2101 accatccata gatactaaaa aaatagtttc atatttacca gaaagaactt atttaaatga
     2161 ttggatgaag gtatcagatt tacttaagtt tttctatgat ttttatagtg attttgatgt
     2221 tagaagagca aatgagatga ttaaaagctt agatattgat gtaaatgaaa agctaaagac
     2281 aatgtctaaa ggaactaaag aaaaagttca acttatttta gtaatgtcaa gaaatgcgag
     2341 catatatata ttggatgaac ctataggtgg tgttgaccca gctgcaaggt catatatact
     2401 taagactata ttaaaaaatt attctgaaga tagtacttta ctgattgcta cccacttaat
     2461 aagtgaaata gaaaacatat gtgatgagat aatattcata tcaaaaggag aaatagtgtt
     2521 acaaggagac gttgaagcaa taagagaaga aaaaggaaaa tctatagatg cattatttag
     2581 ggaggaattt aaatgttagg taaattatta aaatatgagt taaaagcaag tggaagaata
     2641 tttataccgt tatatatagc catattgata gtagctgtat ttaatggaat atttatgaat
     2701 acaaatatac ttcaggtaca gggaatagga atactagtat taacaagtct ttttatggca
     2761 ttaggagttt taactattgt agttacaatt caaaggttta gaaaaaatct tttgggggat
     2821 gaaggttatt taatgtttac tcttcctgtt agcactagtt cacttatatt atctaaatgt
     2881 ataacagcac ttatatacgc tgtacttagt tttatagttg cagtattcac ttttggagtt
     2941 cttatgcttt ttggtacttc tggaatttta ctacctgaaa ttttagattt gtttaacact
     3001 agttttaaat ggatttctga aaacttttta gatattttat tattagtagt agtaatgttt
     3061 atttcatatt catcatttat tttgttatta tatacatcaa tctcaatggg acaattgcca
     3121 aaattcaata aacatagaaa catagtggca tttgcttctt ttatagctat aaatatagta
     3181 atatcaattg ttggagatgc agttggaagc atcttaccaa atgaagatac aaatatggta
     3241 tatcatcttt atcagagtcc tagttttatg cttgcaatac ttggtagtct tgtagttgca
     3301 atagcgttat ttttcgctac aaaatttata ttagataaaa aattaaattt agaataaatg
     3361 tttttatatg gagctatctt ttgagtaatg ttaaaagatg gctcttatta tttatgtagt
     3421 taattcatgt atataatatg aatagtgttt tatttctagt taaatatata catatgttgt
     3481 ataatgatat tgcgtgtaaa aatttgtaag aataactata cactttatat aagttatatc
     3541 aagaaaattt aaaaatatat ggatttgagg gagaagatat gaaaagttat acaaaggcta
     3601 agtggagtgt ttggggtata attacatttt catttgtttt agtattattt ttaaggatgt
     3661 caactgctgt tgtttcagac aatttagcaa atgagttagg ttttaattct atacaaattt
     3721 caaatatagc atccttttgc ctatatgcat atgcatttat gcaaatacct gcaggaattt
     3781 tgattgataa atatggagca agaaaaatta gcagtcttgg aataataatg gcaagtttag
     3841 ggtcaatttt atttggtttg attcaaagta tagaattagc atatatttct cgtgtaattg
     3901 taggggcagg aacatcagta atattacttt gtattttaaa aattcaaggg agatggttta
     3961 ataagtcaga atttgcttct gcaacagcta aattttcatt tgtaggaaat ttaggtggag
     4021 ttcttgctac ttttccactt gtgtttttgt ctgaacttgt tggttggaga aattcatttt
     4081 tattaatcgg tataattggt gttgtaattg gatgttttat gtatattatt gttagagata
     4141 caccaaaaga gtatggattc aatgtagata ctgaacccta tgaaaagtca gagaaggtta
     4201 atattgttga tggtattaag tctgttataa agaacaaatc aacatggtat aattcaatga
     4261 taatgttctc ttttgttggt cttacaagtg catttataag cttgtgggga gtaagataca
     4321 taatggacgt ttatggtgtg agtaagagtt tttctgcgtt tatagtatca ttttttacat
     4381 atggatttat atttggttca ataattatgg attttgtatt tgctaaaata aggtctagca
     4441 aatttaatat aataaaattt ggagcaatga tagatttgtt tatatggatt gtaattgtag
     4501 ttgtatacca ggtaaaacca ccaattatag ttttaccaat atcattcttt attatgggat
     4561 gtatagttat gtcacatcta caggttttta atgatgccaa gtataaaaat aaagaaattt
     4621 attctggact ggcaactagt gttattaata cttttgaatt tataggaagt ggaattatta
     4681 atttgattat agctatctct ttacaagtaa attcatataa tatagttgat ggatataaaa
     4741 aaggttttgt agtatttata gtactaagta ttataactat agtatcatct catatagggg
     4801 taaaaaatga tgattttaag gctatatagt gttttattaa tagtttaatt tagtattata
     4861 ttttgcatat atataacaaa tagtttgcat tttcctaata tgtataattg gaatataaat
     4921 cttgtttact atgtacaaat ggtattgtat tatattaaat agaaaataag aactttaaac
     4981 tataatattg gttctaaaat ataaggtaag gagtttttta tggatacgac gaaaatagat
     5041 gatataagca agttagattt aaataattta gatattaata agatggatat gggaggtata
     5101 atggaaagtc tgctagaatg ggctactaca agcggagtaa aacttataat aggcatactt
     5161 atactttcta ttggatttaa aataataaaa aaatttgtaa accatgttat gttattactt
     5221 agtaaaagag atattgattt aactttaaga aagtttttaa agtctcttct tttgagtgta
     5281 cttaaagttg ccgttataat cattgttctg gaatattggg gaatgagttt gtccagtttt
     5341 gctgctgtga tagcatcagc aggggtagca ataggtttgg ctttacaagg tagtctatca
     5401 aattttgctg gaggatttat tatactattg attagacctt ttaaagtagg agattacgta
     5461 gaagcagcag gtcatggagg aacggttgaa caaataggtc ttttttatac acaattggtt
     5521 actccagata ataaacagat acttatacct aatggttctg tatcaaatga tagtctaata
     5581 aactattcag caaagaatac tagacgagtt gatttgactt ttagtgtagg gtatgaagat
     5641 gatatactac atgttagaag agtacttaaa gatattgtta ataatcataa attaataata
     5701 aatgaaccag aaccatttat aggagtagta gaacatggag ataatgctat taaatttgca
     5761 actagagttt ggtgcaagac agaagattat tggacaattt atcttgattt actagaagag
     5821 gttaaagtta ggtttgacga agaaggaatt acgattccat atcctaaaat ggatttaacg
     5881 gtaaaagaat taaacaaaat ttaatttttt aatatagtgt ttaaatttaa gaatatcata
     5941 tagatatact tataaggttt gtaaaaaatt aataagtact gttattttgt aagtaaaaat
     6001 ataaaaaaat gggagtgtct caaaatgaac tttttagttc atgaggcact ctttttgtac
     6061 gaaataaaat atatttttat tatttcaaca atgaaaaaga ggcttgtttc cattttgaga
     6121 cagtataatt tttatgtaac tttaatttta gtaaatataa agttttaaaa ttgataaaat
     6181 aggaggaatt tttaatataa aagggtaaat aaagggttta gagggcataa tatagaataa
     6241 attaatatat ttaaggaggg tttttatgtg taaaaaaacg tatttattag tggtaagtat
     6301 gcttatagcg ggtttattaa ctgcatgtag tgggagtcca actaaggatt caggacaagc
     6361 acaagataat acaaaaaagg aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa
     6421 taatgaaaat ttaatggaac aagattttaa agtgccatac acagatgcaa taaacatatt
     6481 taaagataaa tataaggatg ctgatatagt agatttaagt cttgagagag atttaaataa
     6541 atttgtttat actgtggaag gtgtagatga taataatgaa tataagatga aaattgatgc
     6601 taatacaaaa gatgtattag aagataaaac agaaaagttg gattcagaag atttaaatgg
     6661 agttgctaga aaagaaaaat tagatttaaa tgacataatg acacctcaac aagctatgga
     6721 aattgcatta aaagaacaaa atggaatagt taaagaatgg agtttagaca aagatttaga
     6781 tgttactttt tataaaataa gaatagataa agataaaaat gaatatgaca taaaagtaga
     6841 ttctaaaaag ggaactgtat tgaaggtaga aaaagaagat tagaagattt aaactatctt
     6901 taaaataatt tcttttttga ataaattttg tttaaaatat aacaagcagg gtataaagag
     6961 actaaataac tattttagga ggagtacggt atgtcattga aatcactaaa aataaaagaa
     7021 aatttatatt gggtaggttc gcttgaccct gatttaagag tatttgacat aattatgtat
     7081 acaccttatg ggactactta taattcatat gtattaaaag gaacagaaaa aacagtttta
     7141 tttgagacag ttaaagataa gcattttgac aattatattg aaagattaaa tgatttaaat
     7201 atagattttg aaaaaataga ctatattgtt gtaagtcata ctgaaccaga ccatgcagga
     7261 agcgttgaaa agcttttgga tttagctaaa aatgcaaaag tagtagcttc agaaacagca
     7321 attaaatatc ttaaggaaat cgtaaacaaa gactttgaat atgttgcagt tacagatgga
     7381 gatacattat caattgggga caaaacttta gaatttttct ctgtacctat gcttcattgg
     7441 ccagatacta tatacacata tataaaagag gataaaactc ttgtaacatg tgattcattt
     7501 ggtagtcact atagtaatga taagatagta aatacacttg atgaaaatga agaaaaggat
     7561 tacttggatg ctttaagata ctattatgat tgtataatgg gtccatttaa accatctatg
     7621 gtaactgcta tagaaaagat aaaagattta gatatagata ctgtatgtcc aggtcatggt
     7681 ccagtattaa ctgaaaatcc tagaaaaatt atagaccttt attacaactg gagtgtaaat
     7741 gaacaaataa agttagaaaa agaagttaca atttgttatg tttctgcaca tggatataca
     7801 aagattatgg cagaagctat aaaagcatat attgaaaaaa atagcaacta taaagtaaat
     7861 ttatttgatg taatagagca taagcaagaa gatattttgg caaaaatagc agtatcacaa
     7921 ggtgtattat ttggaacacc aacaatatta ggagatgcgt taaagcctat atgggatata
     7981 ttaatatcgt taaacccagt tcttcatggt ggaaaagttg cttcagtatt tggttcatat
     8041 ggatggagtg gagaaggtat agaaaatgct atggagagaa taagtcagct tagaatgaca
     8101 gcagtaaaac cttttgctgt taactttaaa ccatcaaatg aagagattga taaattacat
     8161 tcttatacag gtaaattcct agataagtta aattctactt ttggaagtaa aaagaaaact
     8221 aagaaattta aatgtgtaat ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta
     8281 tgccctgtat gtggagctaa ggaagaccaa tttatagaag ttgaagaaga tgaagttact
     8341 tttagaaaag atactgatga atatttcgta atagtaggaa atggagcagc tggtttttat
     8401 gcagcagatg ctataagaaa aagaaataaa acttgtaaga taactatgat ttctaatgaa
     8461 gatgagttga cttactatag acctgcactt tctgatggta taaatgaaga acttggttca
     8521 gatttctaca tggaagataa agattggtat gataaaaata atatagtagt aatattagga
     8581 acgaatgtag ataaattaga tgaagtgaat aaaactatta tagtgaatga tggagctatt
     8641 aaatttgata agcttgtaat agctacagga agtagaaact ttataccacc aattaagggc
     8701 catgatttag aaaatgtgtt tactcttaga aatataaaag atttatatag tgtaaaagaa
     8761 gcactagaaa aatctaagaa agttgtagtg attggtggcg gtttattggg acttgaagct
     8821 gcatgggaat ttagactaaa aggattagaa gtagtagtag ttgaagccat ggatagcata
     8881 ctatcaaaac aacttgataa agaaggtagt aaaatattag aacaatgtgt aagagataca
     8941 ggaatagatg taagattggg tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa
     9001 aaagttgtat ttaaagatgg agatagtgtt gactgtgata tggtagtatt ttcaattggt
     9061 gtaagagcaa acactcaaat ggttcaggat acatctgtaa aaattgatag aggtattgtt
     9121 gtagataaga cattacagac aaatgttaaa gatatatatg cttgtggaga tgttgcacaa
     9181 gtgggaaata ttagtttagc aatatggcca tcttcagtag aaatgggaaa aatagcaggg
     9241 gcaaatgcaa gtggagataa tttgacattt gaaagtgaag tatatccagt ttcattagat
     9301 gcaatgaatg taaaagtatt tagtattggt aatatacaaa attttgataa agaaatttca
     9361 tcaaaagatg aaggtcaaag aatatataaa aaattattta tgaaagatgg ttcattagtt
     9421 ggagctatac ttataaatga tttaagctgt actgttaaat tgataagatt aatatctgaa
     9481 aaaggtgatt ttgaggatat tatgaaggct gatatactat aatttaaata cattaaaata
     9541 aaaaggagtt gtctcaaaat tgattttttt ctaatcaaat tttaaattca actccttttt
     9601 tgaattttta tataaaataa catggaaatt ttagtaatgt ctgtggttac atacataggt
     9661 ttaattgttt tttatatgga ttgtctctgg tataataaat tgaattgtgt ttgactgata
     9721 gatattttta ttgaaagtta atacctgtta tgcgataata aggacgtgtt aggggagttt
     9781 ttataataac aattaatctc agtttttgtg ttccaatagt agcatcaaca cattctgatg
     9841 tagtagttct tttcttataa aaattagtta caaatatgct tgtaattatt tgatatgtaa
     9901 atttaccaat attataaatg attacctcga taataaggag agtgaaaaat aatctatgaa
     9961 cgaaaatatt ttgattgtgg atgatgaaaa agaaatagca gatttaattg aagtatattt
    10021 aaaaaatgaa ggttttacag tatacaagtt ttatacaggt aaggaagctc tagaatgtat
    10081 tgaatctaaa aatttggata tggctatttt agatattatg cttcctgata ttgatggatt
    10141 tcatatttgc cagaaaattc gagaaaaata ctattatcct atcattatgt taacagctaa
    10201 aattgaagat atagataaga taatgggttt aaccataggt gctgatgatt atataacaaa
    10261 gccatttaac cctttggaag ttgttgcaag agtaaagaca caattaagaa gatatatgcg
    10321 atataataat tcttatgaac aacagtcgat aattgttaat gagtatgata ttaaaggatt
    10381 gattatcaat aaagaaacac ataaatgtag tttatttggg aaagaagtag cattaacacc
    10441 gattgaattt tctattcttt ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga
    10501 agaattgttt gaagctgtat ggggagaaaa atatcttgat aataataaca cagttatggc
    10561 tcatataggg cgattacgtg aaaaaattca tgaaccttct aaaaacccga aatttataaa
    10621 aactgtatgg ggagtaggtt atacaattga aaagtaaaaa tattaaagtc aatcctgact
    10681 ttactcaagt gagaaataaa atttttatga gaacagcttt aatggcattt atagccatta
    10741 ttttcatatg gattttatat tcttttatat tgtatggtaa gttttctatc tggatagtat
    10801 cattctttga aaatgctttt cgtatggaat ataaagctgc attgaattta tatcagcaga
    10861 ttttccgtaa tagcatggag ctcttcatga tactagctat ggtgattgtg ttttttataa
    10921 tattccgtgt ttatttaaat tggtttacaa aatattttag tgaaataaat caaggtattg
    10981 actctcttat caaagaagat gttggagaag tggctttatc tccagagtta ttggcaatag
    11041 aaaagaaaat aaactcaatt aaacatatac ttgaacaacg taaatttgag acacaaatgg
    11101 cagaacaacg taagaatgaa cttattgttt atcttgcaca tgatttaaaa acaccactta
    11161 catcagttat agggtatctg acactacttc gagatgaaag tcagatttct gaagaactgc
    11221 gaaaaaaata tttatctatt tcgttggata aggcagagcg tcttgaagat ttaattaatg
    11281 aattctttga aattacaagg tttaatttat caaatatatc acttgaatac agtacagtaa
    11341 gcttaactcg tatgcttgaa caactaacat atgagtttaa acctactttg atgagtaaga
    11401 atttaaaata tacactgaat gttacaccaa atatgatgat aaggtgtgat gttaataaaa
    11461 tgcaacgtgt atttgataac ttattacgaa atgctgttta ttatagtttt gaagatacta
    11521 ctattgaaat aacagctgta caggaggatg attatataaa gcttaaattt atgaatcata
    11581 gcaatacgat tccagaggaa aaacttgagc gtatttttga acaattttac aggttggata
    11641 catctcgtaa ttcaaacagt ggtagagctg ggttgggact tgctatagct aaagaaatag
    11701 tggagcttca taacgggaca ataacagctc atagcgagaa caatataatt acctttaagg
    11761 ttattattcc gattgtatga aatttgtaag aatttaagta taaaaaaata ggaaattctt
    11821 taggataaat aaataaagag cactcttgtt attgatataa ttactgtatc aaaacaagag
    11881 tgcttttatt ttgatacagt aattatatca atagaaaaat gtgaaaatat attatgtgga
    11941 acagatgcaa ttgtaagtga aggttgattg gatttgaaat agaaaaagta ttttaacatg
    12001 tttctgtaga ttgtataaaa ataaataaat caacaaagga gaatcaaaga tgaataataa
    12061 aaaaattgca gttatatttg gtggaaactc aacagaatat gatgtatcat tacaatcagc
    12121 ttttgcagta cttgagaatt tgaatacgga aaaatatgat attataccaa ttggaattac
    12181 aagggagggt aattggtatc attatattgg agattataat aatatccaga ataacacttg
    12241 gtttgaaagg gatactgata taattccagt tattgtttcg cagaaccgtg tcaaaaatgg
    12301 tattattgaa tttttaattc ctgatgctgt ttctcagaat catattccaa atagtgctat
    12361 tgaatcatgt gaaaatgata ttaaatacac taaacttgat tttgtatttc ctatattgca
    12421 tggaaaaaat ggagaagatg gaacagtgca ggggttattt gaattggcag gtatacaggt
    12481 tattggatgt ggtactcttt cttcagcatt atgtatggac aaagacaaat cacataaact
    12541 tgttcattca gaaggtatta aagtaccaaa atcaatatta ctaaaatact cagataaatt
    12601 taatgtggct gaactgtcaa aagacttaac ttatccatta tttataaaac ctgtgcgtgc
    12661 aggttcatca tttggtatta gtaaaattta taaaaaagaa gagttacaag tagcagtaga
    12721 cttggctttt gaacatgata atgaagtaat aattgaggaa aatattgatg gttttgaggt
    12781 agggtgtgca gttttaggaa acgatgactt acttgttgga agagtagatg aaatagaatt
    12841 gtcaaaagga ttttttgatt atacagaaaa atatacattg aaaagttcaa aaattcatat
    12901 gccagctaga attgatttgg attgtgagaa aagaatacaa gagacagcaa aaattattta
    12961 tagaactctt ggatgttctg gatttgctag agtggatatg tttttaactc catctggtga
    13021 aattattttt aatgaagtca atacaattcc tggctttact tctcatagtc gttatccaaa
    13081 tatgatgaaa ggtattggat tgtcttttga agaaatattg gacaagataa ttgaggtgta
    13141 cataaaatga tgaataatat tttattagat aaaaataaag aagataaaat gacaaatact
    13201 atcttattga ctaaagataa agtttatgat gggaatctaa ttctagtaaa tgctttttta
    13261 ccagttaaaa catctgaaga tatagattta attcctgtag atacaaggtt tccaagtatt
    13321 cttatgaaac gtgaagctac aaacatttta caaaatattt taaaaagtat atgtggaata
    13381 aatgaaatag tacctgtaag tggatatcgt actgcagaag aacaacaaga tatatattca
    13441 agttctctgc gtgacaatgg aaaagacttt actaaaaagt ttgtagcact tccaaatcac
    13501 agtgaacatc agacagggct tgctattgat ttagggttaa agaaagaagt tatagatttt
    13561 atctgccctg aatttcctta taatggtatt tgtaatgagt ttcgtaaggc agcactacat
    13621 tatggtttta tagagcgtta tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa
    13681 ccatggcatt ttcgttatgt gggatatccg cattctgaga ttatcaataa aatgaatctc
    13741 gctcttgaag aatatataga atatattaag agattttcgt atacaaattg tttaaaaata
    13801 gaagataaag aaaaaattat agaaatcttt tatgtgccta ttcatactaa agaagaagtg
    13861 agaatctcta tacctgaaca atgtgtatat caaatatcgg gtaacaataa ggatggtttt
    13921 attgtgacat tttggagaaa tagaaaggac taagaaatgg aggaacaaga atgatgagga
    13981 ataaatctta tgcagggctt gattatttta ggattatagc tgcattactt ataattgcaa
    14041 ttcatacatc tccattatta tcatatagta aaatggctga ctttgtgtta actagtataa
    14101 ttgctcgtat tgctgtgcca ttctttttta tgctatcagg cttttttcta atttcaacat
    14161 ataattataa ttgtaataaa ctgaaatctt ttctaaaaaa tgtattattt atttacggga
    14221 tagctattgt aatttatatt cccttaaata tttacactgg atatttttcc atgaataatt
    14281 tagtgcccaa tattattaag gacattgtat ttgatgggac tttatatcat ttatggtatc
    14341 ttcctgcatc aattattggt gcaagtattg catactttct tgtaaaaaaa ctaggtttaa
    14401 agtactcact agtaatcact attgttattt atgtaattgg tatgtttggt gatagttatt
    14461 atggtttttt agaaaaaata ccactattaa agaatttata tgggtatatt tttgaagtat
    14521 cagattatac acgaaatggt atattttttg cacctgtatt tttcatactt ggtggaataa
    14581 tatctaataa atctaattat atgtcaacta aaaatagctt gattggattt cttatatcat
    14641 tcttacttat gttatgtgag ggaatgtttt tacataaact aggggtacag cgtcatgata
    14701 gcatgtatat tatgttattg ccatgtatgt atttcttatt tacagcctta acatttttga
    14761 aaggttctcg agctaggtta ttgcgaacat catcattaat tatttatatt atacatccta
    14821 tgatgattgt tgttattcgt atgttttcta aaatattcgg aatacaaact gtcttggttg
    14881 acaatagcct tattcattat atagtggtaa gtatagcatc ttttatagct tctataatta
    14941 ttatattgat tcttaaacat ctaaaaaaag acttgaaagg agaacataag gtaaatacca
    15001 atagagcttg gattgaaatt gatttaaata atttggaaca taatgtaaat atgatacaaa
    15061 aagctatgcc agaagattgt gaattgatgg cagttgtaaa agctaatgcc tatggtcata
    15121 gtgcttttga agtagcgact tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa
    15181 caatagatga ggggattgat ttacgtcgat atggaatact aggagaaata ttgattcttg
    15241 gatatactaa cccaataagg gcaaaggaac ttcataaata caatcttaca cagagtgtta
    15301 ttgaccttaa tcatgctatc tctttaaaca aacagaaata caacataaaa tcacacatta
    15361 aaattgatac aggtatgcac agactgggtt ttgatgttaa tgatgtgagg tcaattttaa
    15421 aggcatttaa attaaaatac ttagatatat gtggcatatt tacacacttg tgtgtttcag
    15481 atagtctgaa agatgaagat gtagacttta caaataaaca aattgaatgt ttttataagt
    15541 taattgattt tctgttaaaa aaaggtataa aaattcctaa agtacatatt caaagtagtt
    15601 atggtttttt taattatccc aatcttaagt gtaattatgt aagaattgga attgcattat
    15661 acgggatatt aagttctcca aatgatacga ctaaattgca acttgattta cgacctgttt
    15721 tatccttaaa atcacaagta attcttatta gaaaaataca aaaagatgat agttttggat
    15781 atggaagagt ctttattgca agcagagata gcgtaattgc tatattatct attggctatg
    15841 ccgatggtat acctagaaat ttatcctgtg gaaaaagtta cgtaataata aatgattgta
    15901 gagcagcaat agtgggtcga atttgcatgg accaacttgc tgttgatatt acagatatac
    15961 ctaatgtaga agttggtaat acggcaataa taataggaag agataattta gctgaattat
    16021 ctgcttctga agttgcaaat aactcatgta gtatatcaaa tgaattattg agcagagtag
    16081 gaagaaggtt aaatgttata aagaagtaac tcaattaaat agtaatattt gttatactgt
    16141 gtaatttatt ttactctgac tagacatttt tagtaatatt atgtctaata tatatttttt
    16201 ataa
//
LOCUS       DAGSHT010000067        16187 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGSHT010000067 DAGSHT010000000
VERSION     DAGSHT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.069"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..728
                     /locus_tag="MJB72_001932"
     CDS             <1..728
                     /locus_tag="MJB72_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY8263040.1"
                     /translation="ITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCSTDNSPNIVK
                     SYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKLEKQVNFML
                     ENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVIDKSKLDFEI
                     RMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMWTWNIYRNI
                     EKIPLYKAIYYFINYGINGIKKS"
     gene            750..1658
                     /locus_tag="MJB72_001933"
     CDS             750..1658
                     /locus_tag="MJB72_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY8263041.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            1706..2458
                     /locus_tag="MJB72_001934"
     CDS             1706..2458
                     /locus_tag="MJB72_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY8263042.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            2412..3779
                     /locus_tag="MJB72_001935"
     CDS             2412..3779
                     /locus_tag="MJB72_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBY8263043.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            3785..4876
                     /locus_tag="MJB72_001936"
     CDS             3785..4876
                     /locus_tag="MJB72_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY8263044.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            4960..6873
                     /locus_tag="MJB72_001937"
     CDS             4960..6873
                     /locus_tag="MJB72_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY8263045.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            7072..7779
                     /locus_tag="MJB72_001938"
     CDS             7072..7779
                     /locus_tag="MJB72_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY8263046.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            8081..10192
                     /locus_tag="MJB72_001939"
     CDS             8081..10192
                     /locus_tag="MJB72_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBY8263047.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(10390..11355)
                     /locus_tag="MJB72_001940"
     CDS             complement(10390..11355)
                     /locus_tag="MJB72_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBY8263048.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(11640..12596)
                     /locus_tag="MJB72_001941"
     CDS             complement(11640..12596)
                     /locus_tag="MJB72_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBY8263049.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            12964..13752
                     /locus_tag="MJB72_001942"
     CDS             12964..13752
                     /locus_tag="MJB72_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY8263050.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(13875..14345)
                     /locus_tag="MJB72_001943"
     CDS             complement(13875..14345)
                     /locus_tag="MJB72_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBY8263051.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            14484..15128
                     /gene="uppS"
                     /locus_tag="MJB72_001944"
     CDS             14484..15128
                     /gene="uppS"
                     /locus_tag="MJB72_001944"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBY8263052.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            15171..16076
                     /locus_tag="MJB72_001945"
     CDS             15171..16076
                     /locus_tag="MJB72_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8263053.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT         6413 a         1811 c         2839 g         5124 t
ORIGIN      
        1 taattactcc catgtataat tctgaaaaat ttattgaagc aaccataaaa tcagtattaa
       61 accaaactta tcaagaatgg gaaatgttaa ttattgatga ttgctcaaca gataatagtc
      121 ctaatatagt caaatcttat atgcaacagg atagtagaat aaaatgtata aagactgaga
      181 ctaataaggg tgtctctaat gctagaaatt tagcactaag taaggcaaca ggacaattta
      241 tagctttttt agatagtgat gaccaatgga atagtagtaa gttagaaaaa caagtaaatt
      301 ttatgttaga aaatgactat gtaatttcat ttacttcata tgaactgatg gatgaaaatg
      361 ataaaaaatt aaacaaagta ataaaagttc cacctaatgt agattataga agattattaa
      421 aaggaaatat cttaggatgt cttactgttg tgattgataa gtctaaattg gattttgaaa
      481 taagaatgag tggtgtaaga catgaagatt atgttttatg gttatcgata ttgaaaaaag
      541 ggcatattgc tcatggaata aatgaagttc tagcattata tagaaaatct agtaattccc
      601 taagtggtaa taaaataaaa gctgctatgt ggacttggaa tatatataga aatatagaaa
      661 aaataccttt atacaaagct atatactact ttattaatta tggaataaac ggaatcaaaa
      721 aaagttagac agagaggaag atttaattaa tgtataaata taaatttact gtatttactc
      781 cgacgtataa cagagctcat ttacttgaaa atttgtacaa tgatttaaaa gctcaaactt
      841 atgatttcaa tgattttgaa tggttgattg ttgatgatgg aagtagcgac agtactaaag
      901 agttagttga aaaatttata tcagaaaata aattaaatat aagatatata tacaaagaaa
      961 atggaggaaa acatactgca ataaatctag gtgtaaaaaa tgcagatgga gagctatttt
     1021 tcattgtaga tagtgatgat ggtttaatta aagattctct tgaaattgct aatgatgagt
     1081 gggattcttt agagaataaa gaaggattta gtggagttgt tggactttgt gtatatccaa
     1141 gtggagattt gatagggaca gaaatgcctg aagataagaa aatatgtcac tttgcagacc
     1201 tatattttaa gtatggcgtt aagggagata aaagcatagt atttgtaact gatgagctta
     1261 taaaatttcc ttttccagaa agaaaagaag tgagattttt accagaaagt gtagtatgga
     1321 atgaaatgtc taaatactat aaagttatat gtgtaaataa acctatgata ataagagatt
     1381 atttggatga tggtttgact aagaatatat taagtaaaaa tgcattaaga ggtagagcat
     1441 tagagttttt atatttaata aatcaaaata catatccatt aagtagatat ccatatatgt
     1501 ggataaaaaa ttacataaat ttagccagat actcattgtt gtctgatagt cattattttg
     1561 gtgaattaag aaaagtatct gataaattgt tgtatttagc attattccca ttaggatatt
     1621 acaaatatat tggacaaaga aaattagtat ctaagtaaac ttgttggtat agtactgcta
     1681 gttgctatat aaggagagta aaaatatgaa tgagccttta gtatcgataa ttactcctgt
     1741 ttataattca gaagagtttt tatcagagac gataaagtca atccaaaatc aaacttataa
     1801 aaattggcag cttttattag tggatgattg ttctaaggac aattcaagtg aaattataaa
     1861 aagttttcga aaagaagatg ctagaataaa atatataaag cttgaaaaaa attctggagc
     1921 tgcagtatct agaaatgttg gtattaagaa tgcggaaggt cgatttattg catttgtaga
     1981 tagtgatgat ttatgggata gtagaaaatt agagattcag atagaatata tgttaaaaga
     2041 aaatgtaggt ttttctttta catcttatag atatatgaga caagatgggt caaaaacgaa
     2101 taaggttgca agagctccta agaaaattga ctatgaagga ttacttagaa atactataat
     2161 aggatgttct acagtggtca ttgataagga gattgtagga gagttcagca tgcctttggt
     2221 aagaagagga caagatactg caacctggtt acagttactt aaaaaggaaa aatatgcata
     2281 tgggattcaa gaagatttag tgaattacag attagttgga aattctatat caagtaacaa
     2341 gataaaagca ttaaaacgaa cttggaatac ttatagaaat gttgaaaatt taagtttacc
     2401 aaaaagtcta tatgtatttt gtttctatgt ttttaatgcc attaaaaaaa gagtataagt
     2461 tttatatact tggaggtaaa aaaatgaaaa tagctatagc tgggacggga tatgtaggtt
     2521 tggttacagg tgtttgtcta gcagaggtag gcaatgaaaa tgtagtctgt gttgatgtag
     2581 atgaacaaaa agttaaatca atgaaacttg gaatagcacc tatatatgaa gaaggcctag
     2641 aagaacttat ggttaaaaat tacaacagag gaagaataga ttacactaca gactataaaa
     2701 aagcttatga agatgtagat gtaatattaa tagctgtagg tacacctgag agaagtgatg
     2761 gttcagccaa cctagattat ataaaggcag ttgcaaagca aatagctgaa actgtgacaa
     2821 aagatactct agtagtaata aaatctacag ttccaattgg tacaaatgaa gagatagaag
     2881 attatataaa tagaaaccta aaaaatgatg taaaagtaga acttgcatct aatccagagt
     2941 ttttatcaca aggaacagca gtaaaagata ctctacatgc ttcaagaatc gttataggtg
     3001 tagaaagtga atgggctaga gaaatattag aggaaatcta taagcctttt aatcaaccta
     3061 taatagttac aaatagaaat agtgctgaga tgataaaata cgcatcaaat aacttcttag
     3121 ctcttaaaat atcatttatg aatgatattg caaatctttg tgaaatagca ggagctaaca
     3181 tagaagatgt agctttagga atgagttatg atgatagaat aggaagtaag tttttaaagg
     3241 cgggaatagg atatggtggt tcttgctttc caaaagacac aaaagctctt cactggttat
     3301 ctgaacataa tggttatgaa ttaaaaacag taaaagctgc aatagaagtt aatgaaagtc
     3361 aaaaacttaa attagttaga aaggcatcta agttagtaga gagctttcaa gatatgaagg
     3421 ttgctgtttt aggtttgact tttaagccag acactgatga tataagagaa gcaccatcta
     3481 taccaaatat aaaatatttg ctcgaaagag gggcaagagt tactgtatac gacccaattg
     3541 gtgtaaataa tactaagaaa atatttggtg actctatact ttatgctgat tctgtagatg
     3601 atgcgataga agatgcagag atatgtttta taatgacaga atggaaagat gtattaaatt
     3661 atgatataca taagtataag gaaaagatga aaaatccttt agtccttgat ggaagaaatt
     3721 gttataaaat taaagatatg gaagagttag gaattgagta ttattcgata ggaaggtaaa
     3781 tattatgaag actgtttgtt atttagctga tgggtcaaat cctcatactt taaaatggtg
     3841 tagttttttt aaaaataaag gatatgacat tcatgtaatc tctttaaatg gaggtcacat
     3901 ggaaggaata aaaatttata attttggttc taatgtagaa gaactaaaaa atgaaaatat
     3961 ttctaaaaaa acgggatact taggttcaat aaagcagata aagaagctag taaatgaaat
     4021 aaagccagat atattgcatg cacagtatgc aagtagttat ggatttatag gaagtctact
     4081 tgggtatcat ccatatatta tttctgtttg gggtactgat atttatgatt tccctaataa
     4141 tggttttata caaaaacaaa ttataaaaca taactttaga aaagcagact atatcttttc
     4201 aaatagtaga gatatggcta aagaggctaa taaatataca gctaaacatg tagatgtaac
     4261 cttttttgga gtagatatgg ataggtttaa gcctatggaa gtagaaaaag aagacgcttt
     4321 tgtaatagga attataaaga gtttagaaaa aaaatatgga atagaatatt taatacaagc
     4381 atttaagatg cttaaagatg aatataaaga taagaaaata attcttaaaa taggtggctc
     4441 tggaagtcag atggacaacc taataaattt agctaaagaa ttgggtatag aaaatgatgt
     4501 tcagttttta ggaagaatat ctccagaaaa tgtaagcaag acttttaatt cttttgatgt
     4561 aactgtattt ccttctttaa gagagggatt tggagtggca gctatagaat cagaagcatg
     4621 tgaagtacct gttatagtta caaatgtagg aggacatcca gaatctgtat gggaaaatga
     4681 aactggctta attgtagaac caaaacaacc agaagaaata aaaaatgcta taataaaact
     4741 aatggaaaat gatgaactta gacttaatat gggtaagaaa gggcgtcaat ttgttagaga
     4801 aaattatgag gttaatttaa actttaatga tatagaaaaa atttatgatt ctatatttga
     4861 taaatataaa aaataaaatg aaaaaataca gcaaaagata gatgttgttg gctatgctta
     4921 ggtgcgtagc ttttgctgtt taaaggaggg cttctagtta tgcgagaaag tatagattta
     4981 gagaaagaaa gcttttttaa taggcttttt aaaaaatata aatttagaca tttaacacta
     5041 ttaattctag atatttgtag cgtactagta gcgtttcgaa tgtctttaag cttgacaggt
     5101 aatttaaatg catttaccac taatgatata ataatttcag cgtgtatata tatagtactt
     5161 catatagtat catttagatt gtttaagtgc tacaacacat tatggagata tgcaggagaa
     5221 gaagaaatta tatctatttt tgtggcaact ttagcttatt taattccgat ttatataata
     5281 aataaattat tagggtttga ttatcctata atgttttatg tattaaatac aatatttatt
     5341 ataatgttta caagtggcgc aagaatagcc tatagagcta taagaatagt catgaacaag
     5401 acatattcta gaggaaaggt ttcaaatatc cttataattg gtgcaggaga tgcaggagaa
     5461 atggttatac aggaactaaa aagaaatcca gaacttaaaa aagtagcagt ggctataatt
     5521 gatgatgata aaaataaaat tggaagaata atccacaatg tgaaaattgt aggaacaact
     5581 tcagatataa aagatgtggt agaaaagtac aatgtagatg agataatctt ttcaatagca
     5641 aacatagaga aacgtagaaa aaaagagatt attgatatat gtaaaaacac taactgtaaa
     5701 ataaagacaa taccaggaat ttacgaaatt atagatggaa aagttgatat aaaacagatt
     5761 agagaagtag agatagaaga cttgttgggt agagagccta taaaaacaaa tttaagagag
     5821 ataagtaatt acattgaggg aaaagtaatc ctaataactg gaggaggagg ctcaattggt
     5881 tcagagcttt gcagacagat tgcaggattt aatcctaaag agcttattat agttgataat
     5941 tatgaaaata atgcttactc aatacaacaa gaattgctta gaaaatataa aaataaatta
     6001 gatttaaaaa cagtaatagc atcaataaga gaagaaaaaa gaatggatga aatatttaat
     6061 aaatataaac cagaagtggt atttcatgca gcagcacata aacatgttcc tcttatggaa
     6121 tcgagccctg gcgaagctat aaagaataat atatttggaa ctttaaacat agctggcttg
     6181 tcttcaaaat atagagctaa gaaatttgtc cttatatcta cagataaggc tgtaaaccct
     6241 actaacataa tgggagctac taaaagagct gctgaaatga ttatacagac tatgaatgct
     6301 gaaagccaga ctgaatttgt tgctgttaga tttggaaatg tgcttggtag taatggtagt
     6361 gtaattccac tttttaagaa gcaaattgaa gatggcggac cagtgacagt tactcatcca
     6421 gatattatta gatattttat gacaatacca gaagcagtag gcttagttat acaagctggg
     6481 gctatggcta aaggtggaga aatatttgta cttgatatgg gagaacctgt aagaatattg
     6541 gatttagcaa aaaacttaat taaattcagt ggatttgagc cagatgttga tataaaaatt
     6601 gaattttctg gtcttagacc aggagaaaag ttgtatgaag aactacttat gtcagaagaa
     6661 ggtctgttgg atacagagca taaaaagatt tttattggta gaccaataga tgttgataga
     6721 gaaaaaataa caaaatactt aaaactttta agagaaatta ctaacaatga agaggttgaa
     6781 aaaatagatg gtattatgag ggaattagtg ccaacttata ttaaaccaga agatgctaat
     6841 atcaaagaaa ttgcaacaac tagagaaaag tagaatatat ttctaatttg taaccagggg
     6901 gagcaaaaag aaacaaatat atactaaaaa gaaacaaaag attacaaaaa gattacaaaa
     6961 agattacaaa aaaggttgca atttattttg atgtaagata gaatgttaat taggaattgg
     7021 aaacgaagga acaaaatgtt aaattttcga aaggattagg tatttgagat tatgatgaat
     7081 attaaaaata aaaaacatat attgaaaaaa tttatagcta tggtgttaat agcaggagtt
     7141 gtcacagtag aagcaggagc aataacggct agtgcagcag agccaactaa ttcacctatg
     7201 agtgcaacag tagaccagtg tgatttttta aatgtaagaa gtggagctag tgcaaatgat
     7261 gcagtagtag gaaagataaa cacaggagac aaagttgaag ttttagaatt acattcaaat
     7321 ggatggatta aaataaaaag tgttgacaat gttacaggat gggtaaatgg agattattta
     7381 actattcaag gtggaaatgt agatgcaaag gtacaaaatg ttttaaactt agcatttaag
     7441 caacaaggta agccatataa atggggagca actggaccaa attcttttga ttgttctggg
     7501 ttcacttctt atgtctataa aaatggagca ggagttaatt taccaagagt atcaagatca
     7561 caagcaacag taggaaagaa agtatcaaga gcagaattaa aaccaggaga tttagttttc
     7621 tttggttcag gtggttcaat aaatcacgtt ggattgtatg taggagattc taaattcata
     7681 cattcaccac aaacaggaga tgtagtaaaa gttactagca tggctccagg aactaattat
     7741 gcaagaagac ttataactgc tacacgtgtt ttacaataat aatagatatt gatttacata
     7801 ggattgaata ttaacattaa atatataatt acatttaatg ttttacatag agatttaaga
     7861 gtttagtaat attaaaatag tgatgaacgt tataaaaaca gcaaagaaga taaatttctt
     7921 tgctgttttt tttataataa attatttcat attataatca aatatcaaat ataaatttta
     7981 actatattat tcaatatttg acagataata tgaattttag atatacttaa taagaagtat
     8041 attgtagaaa aaataaattt aaaaaattaa gggggatgaa atgaaaaaaa taagtatatt
     8101 agtattatca ttgataatga cacttactat gtgtagtgta agttcatttg ctgattcatc
     8161 aaatgataag gaaatgagag ctgcttggat atctacagtt tataatttag actggcctaa
     8221 gactaagaat aatgaagcca agcaaaaaaa ggaatacaca gatttattgg acaaattaaa
     8281 aagtgtagga ataaatacag cagtggttca agtaagacca aaatcagatg ctttatataa
     8341 atcaaatata aacccatggt ctgaatatct taccggtaca caaggtaaag accctggata
     8401 tgacccatta ccatttttaa tagaagaagc acataaaaga ggaatggaat ttcatgcatg
     8461 gtttaatcca tatagaataa ctatggcaga tgagagcata gataaattgc cagcaaacca
     8521 tccagcaaag aaaaatccaa gctgggtagt taaacatgga aataagtatt actacgaccc
     8581 aggtttacca gaagttagaa agtacatagt ggatagtata gcagaagtag ttcaaaatta
     8641 cgatatagat ggagttcatt ttgatgatta cttctatcca ggagtaagtt tcaatgatac
     8701 tgcaacatac caaaaatatg gaaaaggaca aaataaggat gattggagaa gagaaaatgt
     8761 aaatactcta cttagagatg taaaagcgtc tataaagagt ataaagccta atgttgtatt
     8821 tggtgtaagc ccagcaggta tatggagaaa taagagtagt gacccaacag gttcagatac
     8881 atcaggtaat gaaagttatg taggtacata tgcagataca agagcatgga taaaacaagg
     8941 attgattgac tatgtagttc cacaacttta ctggccaatt ggtcttaaag cagcagatta
     9001 ttctaaatta gtggcatggt gggctaatga agtaaaagga acaaatgtag acttatacat
     9061 aggacaaggt atatataagc aaggtcaatc gagttatggt ggacaaaata tagcaaaaga
     9121 aatagtacaa caagtaacat taaatagaaa gtatagtgaa ataaaaggta gtatgtattt
     9181 ctcagctaaa gatatagcaa atagtacttc tatacaaaag gatttaaaga gtttatattc
     9241 tagtagtgaa gaaccagtta ctccaccatc taatgttaaa gtggagaaat taagaggcga
     9301 cgaaagatat gacacagcag tagcaataag taaaaaaggt tgggctacaa attcagatac
     9361 agtagtatta gtaaatgggt actctatagt tgatggtata acatcaacac cacttgctac
     9421 atcaaatgat gctcctatac tattggtaaa taaggataat atacctacat ccactaagaa
     9481 tgagttaaaa agacttaatc caagtaaagt tattttaatt ggaggaaata actctattgg
     9541 agataaagtt gagagtgaga taaaagatac attgtcaaat gtatcaataa atagagttgg
     9601 tggttcagat agatatagta catctttaat gatagcaaaa gaattagtaa aaactaatcc
     9661 agtagaaaaa ctatatataa caagtggtac aggtgaagca gattctcttt ctatagcttc
     9721 aaaagcagga gaagaaaagc aaccaatagt attagtatca aaagataatg taagtgatga
     9781 agtttacaat tggatatcag atttgaaagt aaaagatgct tattttatag gtggtaatct
     9841 aagtatatct gattcagtaa taaataagct ggataaggtc attacaaatg atgtatctaa
     9901 aaatagaata gctggagaaa atagagaaga aacaaatgga aaagtaatac aaaaattcta
     9961 tccaaatgct gaatattcat caatgtttgt aagtaaaagt aatcaattgg tagatgcatt
    10021 aacatcaggt cctttagcag ctaaattaaa atcaccagta gttatgcttg gaaatagtgt
    10081 aacatctgct cagaaaactg ctttagagcc taagaaaact actctagtat acgaagctgg
    10141 agatggtata aatcaaaaca cattaaacac tttcttaaac ttagttaagt aataaaaaag
    10201 ttaatttaaa atacagagta ttttataaat gtagattaaa atgaaaatta tcatgataaa
    10261 ttagaaaata aaaaagaact atatgcattt tgtaatcaag tgtacacagc aacttgaaaa
    10321 taattatgcg atatagttct tttttagtaa ttaatcttat actttttaat aaactttatg
    10381 ttgaattttt tattgtttaa actctatgtc attaaatata aaatcgtgta atatgtctaa
    10441 agtatctggg tcaaatctaa gaacccatcc agcttttcca tatataccac ctgtactatg
    10501 aatttcatca tcaataggaa attcaaattg cttaatattt aagtcgccca tttttaaaac
    10561 ttgagctcct aaagaaagta ttgcattagg cttcatgtta gttttaacat atggaagaac
    10621 tgcatctaat aaacctggat atttagtaac aggtaaagtt tccgttttct tgattatagc
    10681 ttgtataatc tgtctctgtc tgccatctct agcaaaggct gtatcgttgt gtctgatacg
    10741 tgcaaaagat agtgcctgat atccatttag agtctgcttt ccagagtttc taatgtattg
    10801 tatgctacct ttatcatcac ttttattcca tttataagtt tcaggaataa atttatttag
    10861 ttctcgtatt tctccttttt caacagtaac ctcaacgcca ccaatagcat caattatata
    10921 cataaatgat tcgaaattaa cacaagcata attttgaata tcaatattaa aattctcttc
    10981 aattgtttgt attaataaat cagcttgtcc ataagcataa gcatgagtaa gtttttgttt
    11041 accatgacca gggatgtcta catagctatc acgagctaat gatgtaagtt tgatatcatt
    11101 gtgtttgtta tcaatagtaa gtatcatcat agcatctgac cttgaagaat cttcatttgg
    11161 tctagcatct gttcccataa gtagtatgtt aattatccca tcttcatttt tatggtcatt
    11221 gttatccaat aaatcactac ttatgctcga gtcatgaata gcagataatt tataataaaa
    11281 atatccatag acacttatag ggaatattac taccaagaaa gccagtagta taacaaattt
    11341 ctttaatttt gacaaaagta ccccttcttt cttctttttc ctgtatattt tttaatagca
    11401 tacacttaat tatattaaag tattgagata tattcaatac ttgtttcagt tacaaaaaaa
    11461 agacaatgat ataatttata agaaaattta tcgacctata tatatagtta aaagtatata
    11521 taatatattt tttaaaagta atttatattt taaaataaaa acacacccca taaatacaaa
    11581 gtaatttgca tttaagaggt atgtatttat catcaaatta ttggtttgtt ttggaatttc
    11641 taatcttcaa ccataatatc tttaaatatg aaatcatgca ttgcatctag ttcatattcc
    11701 tcaaaaggta ttacatagcc tgcattacct atttttctac cattttcagt agaaagagga
    11761 aactccatag atttcaaatt taaatttcct atacttaaaa gttcttttcc taaagatatt
    11821 atttcattag gattcatatt agtttttaca tacggaagta ttgtgttaac tagattagga
    11881 tatttagtga ctggtaaatc cttaacacca tttattaaac tctgtataac acttctttgt
    11941 cttctatctc tttcatctgt actatcattt tttcttatac gagaaaatgc aagtgtctga
    12001 tatccattta atttttgctc accagaattt cttatatatt taagagaacc tttattgtca
    12061 tttttattcc atttataagt ttcaggaata aatttattta attctcttat ttcactctta
    12121 tcaacagtaa cagtaactcc acctaaagca tcaactatat ccataaaaga gaaaaagtca
    12181 actacggcgt agttttgtat atcaagttgg aagttttctt ctatagtttc tattaataag
    12241 ttagcttgtc catatacgta ggcatgtgtt aatttttgtt ctccacgtcc tggtatatta
    12301 acataagtat ctctatttaa tgaagttagc ttaagacttt tatttttatt atcaatagta
    12361 agtatcatca tggcatcaga acgagaaact tcttctcctg gtcttgcatc tgtacctaac
    12421 aataatatgt ttgtgatatt ttcttcgttt ttatagtcat tactactaag cgcctctgta
    12481 tttatactat catcttgtat agtttttaat ttagtaaaaa catatccata tatactaaca
    12541 ggaaatgcta taactaaaag ggctagaaat ataacgaact ttttgagtcc tgacaataat
    12601 atcccctact ttctaaattt tttaattaca catagttaat tatattaaaa atttgttaca
    12661 taagcaataa aaaacacaat aaattagcat aagtttgtca taatataatt atttttatag
    12721 cattttatag aagaaagtgt gtagttatta ttgattttat tggaatataa ttgtatgaag
    12781 acttaaattt ttataaactg atgtgtatat tgaaaaaaat attttcctga atgtagttat
    12841 agcatcttat gaaaggtgca aattgtaatt taaaattttt agtgtataat aagaattgaa
    12901 aattcaaacc gataaaaatt aataaaaaat aattataaat taataatttg ggggaggaaa
    12961 tgtatgataa aattaatagc aacagattta gatggaactt tgttagatga aaagagcgaa
    13021 ataaatccag agttttataa agtatttaaa aaattaagag agagagggat aatgttttct
    13081 gctgctagtg gcaggcagta tcaaaatctt ataaaaaaat ttgaagatat aaaagatgat
    13141 atgatgttta tatcagaaaa tggaacacta gtagtatata aaggaaaaga aatattatca
    13201 aatcctttaa ataaagaatt agtaaatgaa atcatagaga caacaagaag tataaaaggg
    13261 aaaaaaattg taatgagtgg gaaaaaatac gcttatatag aaagcaaaga tgaagcgttt
    13321 atacaagagg tcagtactta ttatgcaaag tttaaaattg tagaagattt gacgaaagta
    13381 gaaggagaca tattgaagat agctgtattt gacttcaaag gtgcagaaca taataataat
    13441 atctactttg aaaagttttc tgatagagct caagtatgta tttctggagt ggagtggctt
    13501 gatttaactg caaaaggtgc aaataaaggg agtgccataa aaaaagtaca aaaaatgtta
    13561 gatataaaat atgaagaaac tatggtattt ggtgaccagt taaatgatgt ggaaatgatg
    13621 aaaagtgcct atcatagtta tgccatggaa aatgcaaatg aacatttgaa acaaattgct
    13681 agatttagag caaaaagaaa tactgaaaat ggcgttgtag ataagataaa agaggttata
    13741 aaaattggat agtcatatgt atagatgaaa aagattattt tgaagaaatc aaaaaaatat
    13801 caatgtaatt ttgataaata agtttattta tgcgaaaatt acattgatat ttaataaaca
    13861 tcaataatag atatttattt ttttatgata ttcagtattt tgttttctat ttgagttgca
    13921 ttttcagcag aaattaaata aacatatcta cctgccttct caattatagt attatttgct
    13981 atttcagaat tgattttttc tccatgtcct tctccaaaag gtgcttgtat catatctttt
    14041 ttataatttt ctagagtttt atagatttta tctacctcgt cagtttttat aacgataata
    14101 tcttctagag ataatttttc agaagaatta attacaaaac cttgaattat gtcttcctct
    14161 acatcattaa aatagttaaa atcctttgca tcaaagattt ttgattcctt tataagtaaa
    14221 tcagaagatt ttaatgcttt ttttattttt gatgtagaaa cgtttttgta atcaccactt
    14281 gaagaacaac taaccattcc taatgataag attatcgaaa aaaacaatat taaaattttt
    14341 ttcatgataa acctccaagt tttttaaact ctgtcttaaa ttagtttact acataattat
    14401 ataaaaataa atgtatttaa tgttacagtt ttgattaata atgtaatatt ttgataataa
    14461 tacctattga gaggtgatta gaaatgagaa taccaaaaca tgtaggaatt ataccggatg
    14521 gaaatagaag gtgggcaagt caaaatggta tgacaaaaga taaaggatat gaaagtggaa
    14581 tagaccctgg actagagtct tatagattat gtagagattt aggctgtgaa gaggtgactt
    14641 tttatggatt tacaactgat aatacaagac gtccatcagt gcagacaaaa gcttttacaa
    14701 aagcatgtat tgatgctgca aatatattaa tagatgaagg tgctgcttta ttagttgtag
    14761 gaaatacaaa atctccaatg tttccagaag aattattacc atatactaat agaattaaag
    14821 ccaaagaagg tcaaataaaa gcaaatcttc tggtaaatta tggttggtat tgggacttaa
    14881 ataatatatc aggagtagaa aatgtaaata aaaataatat acaggattac atacagtcat
    14941 ttgatgtatc cagactagac ttgataatca gatggggtgg aagaaggaga ttaagtggtt
    15001 tccttcctgt acaatcaatt tatgcagatt tttatgttat agataattat tggccagatt
    15061 ttaagagtga cgatataaag gacgcattta gttggtatga tacacaagat gtcactttag
    15121 gtggataatt aaaaatattc tgatttaagg ttgtagagtg ttttaaatta ttgaaaaaaa
    15181 aacttcttga tggtaaaata acactaataa tatgtaaaag tgtaaaaatt tataccaaga
    15241 agggtgaaga aatgtcaaaa ggcaataata acaataattc aagaaacaaa tctaaaaaga
    15301 ccagtcatct gaatagaaaa aagcgaaaat taaataagaa aaagttagca gtgttgattt
    15361 gctttacagt tttattttta tttattgcat ttaaggctac tcaaggtgta gtagctttgg
    15421 taaaaagtat ggataaaagt aataaaacta atcaacaaca aaatgttaac tcagaacaat
    15481 ttgaccttgg taatgaagag gaaaataaaa agaaaaaata tactgtattt atagacccag
    15541 gtcatggagg caatgataaa ggaactgaaa gcaagaccag taatagatat gaaaaagacc
    15601 taaatctaca aattgcaaaa aaattagcaa ataaattatc taaacaaaag gatattcaag
    15661 tagtagtatc cagaactgat gatacatata tatcattaaa ggatagagct atcttagcaa
    15721 ataatagttc tgctgatgtg cttgtttcta ttcatttaaa tgcagagaaa aatggtaata
    15781 cagcaacagg tatagaaact tggtatagga ataaagctac agatggttca aaagaacttg
    15841 cacagacagt acaaagtact atagtatcct atgttaaagt tagagatagg ggaatagttg
    15901 aaaataactt tgaagtactt agagaatcaa atatgcctgc tatattaata gagtgtggtt
    15961 ttttaacaac accttcagag gaacaaaaaa taataaatga aaaatatcaa gaccaacttg
    16021 cggaaggaat tgttcaaggt gtactttcat acctagattc taaagggaat aagtaagtta
    16081 actttacttt aatgtgagga taaatttaaa agtttaactg tgtaaattgt attaacttta
    16141 tttcgtaatg ttgcaaacat tgtttgaatt tagaaactat gaaatta
//
LOCUS       DAGSHT010000068        16151 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGSHT010000068 DAGSHT010000000
VERSION     DAGSHT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.070"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..157
                     /locus_tag="MJB72_001946"
     CDS             <1..157
                     /locus_tag="MJB72_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino-acid racemase"
                     /protein_id="HBY8263054.1"
                     /translation="EDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKCLAESVVN
                     KYLKLVK"
     gene            complement(377..994)
                     /locus_tag="MJB72_001947"
     CDS             complement(377..994)
                     /locus_tag="MJB72_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBY8263055.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(1121..2143)
                     /gene="argF"
                     /locus_tag="MJB72_001948"
     CDS             complement(1121..2143)
                     /gene="argF"
                     /locus_tag="MJB72_001948"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBY8263056.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            complement(2247..3434)
                     /locus_tag="MJB72_001949"
     CDS             complement(2247..3434)
                     /locus_tag="MJB72_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBY8263057.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLCSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(3464..4324)
                     /gene="argB"
                     /locus_tag="MJB72_001950"
     CDS             complement(3464..4324)
                     /gene="argB"
                     /locus_tag="MJB72_001950"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBY8263058.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(4339..5556)
                     /gene="argJ"
                     /locus_tag="MJB72_001951"
     CDS             complement(4339..5556)
                     /gene="argJ"
                     /locus_tag="MJB72_001951"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBY8263059.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(5597..6631)
                     /locus_tag="MJB72_001952"
     CDS             complement(5597..6631)
                     /locus_tag="MJB72_001952"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBY8263060.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGSAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(6885..7098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7401..8492)
                     /gene="ytvI"
                     /locus_tag="MJB72_001953"
     CDS             complement(7401..8492)
                     /gene="ytvI"
                     /locus_tag="MJB72_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY8263061.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFVIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     gene            complement(8507..9898)
                     /locus_tag="MJB72_001954"
     CDS             complement(8507..9898)
                     /locus_tag="MJB72_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBY8263062.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFANYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVESRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            complement(9917..10456)
                     /locus_tag="MJB72_001955"
     CDS             complement(9917..10456)
                     /locus_tag="MJB72_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8263063.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLMIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            complement(10477..11802)
                     /locus_tag="MJB72_001956"
     CDS             complement(10477..11802)
                     /locus_tag="MJB72_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8263064.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            complement(11799..12422)
                     /locus_tag="MJB72_001957"
     CDS             complement(11799..12422)
                     /locus_tag="MJB72_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBY8263065.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            12514..13323
                     /locus_tag="MJB72_001958"
     CDS             12514..13323
                     /locus_tag="MJB72_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8263066.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            13757..14119
                     /locus_tag="MJB72_001959"
     CDS             13757..14119
                     /locus_tag="MJB72_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263067.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(14469..15026)
                     /locus_tag="MJB72_001960"
     CDS             complement(14469..15026)
                     /locus_tag="MJB72_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263068.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            15284..15982
                     /locus_tag="MJB72_001961"
     CDS             15284..15982
                     /locus_tag="MJB72_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8263069.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         5501 a         2821 c         1810 g         6019 t
ORIGIN      
        1 tgaagatgtt aaaaatgaat ctatagaaaa tatagtaatt gcttgtactg atttaaatgt
       61 agttttagaa aaatcatatc cttcaataaa tattgttgat tcatcaaaat gcttggctga
      121 atcagttgtc aataaatatc taaaattagt taaataaatt tttcatgcac tatattataa
      181 aaaatatatg ttataagttt attttaaata gtatagactt ataacatata tcatagtttc
      241 ttgacaatat agaaattaac catgtatttc attatattaa taaaaaggat accaaaaatg
      301 gtatccttca taaatattta tatgtttatt tataaaaata atcatatatt tattacttta
      361 ctacacctaa acaatattat tcaagtgtat tcatagagtt agaaaattct tgtcctgctg
      421 taacaagatt agataacaat acatcagcta ctgcatcatt tactgaactt attatccatt
      481 tattatcctt tttagaacat tcaaactcta cattttttgt aacataagtc tctttaacgc
      541 ttttttgttt ttctttcata attgatacta acatttcagt tgttttctca tcagttaagt
      601 cttgtcctga aaaactcata ccaatcatct ttgtaaatgc ctcagctact atttcttgaa
      661 gtaatggtgt tgaatctata aatttacatt ctactttgat tgtagcctta tcacctttaa
      721 cctcgctgtc ttttattgta tatgtgattt tagatgcatt ctgttttaga taatctaaaa
      781 catattgggc atttgggtct tttgtatcta attcctttaa tatatcctta tatttttcgt
      841 cattattttc cattacctta ttcatgcctt caaagtcaaa ctttttcgca ctgttaaaaa
      901 aactatcaat tgtatcttct ggttttgcac ttgaacatgc tgttaaagat attgctaata
      961 aaaaagttgt taaagctaat atgatttttt tcatatctca aatcctccct cttaatttgt
     1021 tgatacataa gttattttaa catataatga gagtttttga ataatatatt taaaatattt
     1081 tataaatttt cattaaagta aagtctatat taaaacatat ctatactaaa ttattgttca
     1141 tatttacatc agtcatagta gaaaccataa cagcttttat tgtatgcatt ctattttcag
     1201 cttcttcaaa tacctttgaa tattttgaat taaatacctc gtttgtaacc tctaattctg
     1261 ataatccgaa cttttcataa atctcctgag caaccttagt ttctaaatca tgaaatgctg
     1321 gaagacagtg taaaaatata acattttcat tttcagtttt ctttatcata tccatattta
     1381 cttgataatc tttaagttga tttattcttg cttcatattg ctcctcttct cccatagaaa
     1441 cccatacatc tgtatatata acatctgcac ctttaacatc atcaatattt tcagtcaaca
     1501 ttatatctcc tttggatttc ttggctattt ccttcatttc cttaaccaat tcttcatttg
     1561 gccaaagttc cttaggtgct agtgctacga aatgtattcc cattttagca catcctatca
     1621 tcagtgagtt tcccatatta tttctagcat caccaacata tacaaattta actttattca
     1681 aatctttatc tacattttca attactgtta aaaaatctgc cagtatttgt gttggatgat
     1741 attcatctgt aagtccattc caaacaggta caccagaata ttttgctaga ctttcaactg
     1801 tttcttgttt aaagcctcta aattctattc catcatagaa tcttcctaga acttttgctg
     1861 tatctgatac agactctttt ttacccatat gacaatcatt tggacctaga taagttacat
     1921 gtgctccttc atcatgagct gctacttcaa atgaacatct agttcttgtt gatgttttct
     1981 caaataataa agctatattt tttccttgta gcaaatcgcc ttttatacca acttttttct
     2041 tagtttttaa ataacttgat aaagttaata aatatctaat ctcttctttt gtaaaatcct
     2101 ttaatgtcaa aaaactctta ccttttaaac ttatattatt catattaatt cgccccttct
     2161 tataaacttt ttaaattcaa ttaaacttgt tagcacttta cttttttatt tacttctaat
     2221 tttttaatta actatttttt gaatatttag ttaatttcta gtaatatatc ttttaggatt
     2281 tcaagagcct catctatttc cttataactg attgttaatg gtggtaaaaa tctcaataca
     2341 ttttttccag cagtcaaaat aagcaatcct ttttttctag ctttctcttc tataatacta
     2401 gattcaatat tagtttcaat tcctatcatt agaccaatac cttttgtctt taatacaact
     2461 ttgttattaa aactttcaat agattttctt atataatttc ctttttctaa tacttcatta
     2521 taaaaaccat ctttagatat ctcatctaat acaactgaag ctgccaccat agctaataaa
     2581 ttacctccaa aagttgtacc ttggtctcct tttcctagta cttttgaaag ttttttattt
     2641 accaatactc ctcctactgg tattcctgct cccaaccctt ttgctacggt aactatatct
     2701 ggttttacat caaaatattc atagccaaaa agtttaccag ttcttcctat accgcattgg
     2761 acttcatcaa aaatcactac aatatccttt tcttgacata tttttactat ttccaaaaca
     2821 taatcttttt caagaacatt aactccacct tcgccttgaa tcgcttcaag aattattgca
     2881 caaactgtgg aatcaagatt attttttaaa tcttcaatat tatttctttc tatatattta
     2941 aaaccttcgg gaagaggata aaaatactta tggtatttgt ccataccagt tgccattaaa
     3001 gacatcattg ttctaccatg aaaagagtct tttaaactta tgatagttcc tctaccatta
     3061 ccatatttgt cactactata ctttcttgct attttgaatg ctgtttcatt tgcttctgtt
     3121 ccagaattac aaaaatatac tttagccatt ccacttgaat taattatttt ctctcctgaa
     3181 cacaacactg gctcattaaa atataaattt gaagtatgta ataatttatc tacttgctct
     3241 tttagagcat tctttaaatt actattacta tacccaagag aagatactcc atatccactt
     3301 gtaaaatcta tgtatttatt gccattagta tcaaaaaaac aacttccttc accataatct
     3361 atgacaaaat tgggttggtt atatgtatct ataaaatatt cattccattt tgaaattgta
     3421 ttgtcgttat tcattcttat tctcccctct taaacttata attttattca ttttcttttc
     3481 ttattaatgt acctattcca ctatcagtaa atagctctgt tattatagaa tgtggaactc
     3541 ttccatccaa aatatgtacc ctatttactc cgttatttaa ggcatctacg catccctcta
     3601 ttttaggtat catacctcca gtaattatac cttcttcaaa taatttatca acatcctcta
     3661 atataacttc tgttattaat gatttttctt catctggctc tcttaacaaa ccagggacat
     3721 ctgtaagaag tatcaattta tctgcattta actctttagc tatagcagaa gctgctgtgt
     3781 caccatttat attataagtc tctccatcat ctccaacacc tatagttgca attactgaaa
     3841 tataacctga ctttagacaa gattctataa gctcaacatt tacttttttg atttctccta
     3901 caaatccaag ttcataattt ttatatgggt cacataaaat catattatta tctattccac
     3961 aaagccctac tgcttttcca ccttttgtat gtattttatt tactaggtct ttattaacct
     4021 ttccagcaag taccattttt acaatttcca tggtctcttc atctgtatat ctaagaccat
     4081 ttacaaattt actttctata tccacttttg ctagcatctt attgatttct gcccctccac
     4141 catgtactaa aactatattg attccaacat aactcattaa cacaaggtct tccataacag
     4201 attcttttag accatctttt ttcattgcac ttccaccata cttaactaca atagtctttc
     4261 cctgatgttt ttctatatat ggtaaagctt ctataagtgt atttgctttt tctatattaa
     4321 tcattaaagt atatcctcct aatttctata acttccattt attcttacat aatcatagct
     4381 taaatcacat ccccatgcat ttcctctggc attgcccata tttaaatcta ctaatatact
     4441 tatttcgtca tcttcaagga ttttttttgc tttttcctca ttaaaatcta atccatttcc
     4501 atttttacat acttcaatat atcctttcat actctcaaaa ataatatcaa ctttttccat
     4561 atctatttct tcacctgcat aacctaaagc acacagtatt ctaccccaat ttgcgtcagc
     4621 tccaaataca gctgtcttta ctaatgaaga agatataact gtttttgcta attttactgc
     4681 atgttcttca cttaatgctc catttatata gcattcaatt agttttgtac atccttctcc
     4741 atcttttgca acaagtttag caagttctat acagacatat gtaagtgcac tcaaaaatac
     4801 ttgataatgt tcatcttttt tgtttattct atcatttttt gcttttccat ttgctagaat
     4861 cattaccata tcattagtac ttgtatctcc atctacacta actctattaa aacttttgtc
     4921 aacagcaatt tttaaagctt cttttagcaa aactccatca atatttgcat ctgtagttat
     4981 aaatcctaac gttgttgcca tattaggatg tatcatgcca gaaccttttg ccatacctcc
     5041 aatagttatt tttttatctc catacattat tgctactgct atttcttttt ttataatatc
     5101 agttgtcata atagcttctc ttgctccaat atgaccttgt ttacttagat tattcactaa
     5161 ttcatccata ttttcttcta ttaaatctat atttaatggc tttcctataa cacctgtaga
     5221 tgcaaccaaa acatcacttt ctttaatatt taatttatca ctggtgaatt tagccatttt
     5281 ataagcattt ataagtcctt cttttccatt aaaggtatta gcattagcac tatttactat
     5341 gatagcttgt gcctttccat ttttaatgtg ttcctgtgta acataaacag gattgccctt
     5401 tactttattt tgagttacta ctgctgctgc atcacataaa acatctgaat aaaccaaagc
     5461 taaatctctt ttttctttat tttttctcaa gccacagtga atccctgaag caaaaaatcc
     5521 ttctgaaact gtaactccac cctttataat ctccatattt atcctcctaa ttatatgagt
     5581 ttagtatttt ttatttctaa aatgatggtg caatattttt taaacctgta ttttcttcaa
     5641 taccaaatat tatattcatg ttttgtatag cttgacctgc tgatccctta atcatattat
     5701 ctatagctga acagattatc aatgtatctc cattttcatg taatgatatt acacatttat
     5761 ttgagaatct aacattcttt aaagaagcta ccttatctaa tggaagaatt tctataaact
     5821 ctttaaattc ataataatca gttaatttcc tatgtatatc atttatagaa atattatctt
     5881 cttttgtaca atatatagtt gataagattc ccctatttac tgggattaag tttggtgtaa
     5941 aaactacact tactttttct ttgcaggatt cacttaaatt ttgttctatt tctggagtat
     6001 gtctgtgttt ccctatttta tatgcagtga tattttcatt aacctccgta aagtgactac
     6061 taatcgataa ttctctacca gcacctgtaa gacctgattt agaatcaatt attatattat
     6121 tattttttat caatttagaa cttagtaatg gcatcaatgg taaagaaata ctagttggat
     6181 aacatccagg atttgcaatt atatctgcat cttttatatc ttctttgtat atttcactta
     6241 atccatatac agctttttta tgtaagattt tatctataaa agatactcca taccatttac
     6301 tatatacttc ttcatctttt attctaaagt cagcacctaa atctataact ttctttttcg
     6361 atttaattgc ctttattaca aacttttcac taactccatg tggaagggct gtaaacacaa
     6421 catcacacat atctataact ttttcatttt ctatacatat catgttattt ttatatccta
     6481 tacttggata tatatcaact atatcttttc caacatacga atttgaacct atcgctgtca
     6541 cttcgacttt atcatgattc attaataatc taatcaactc tgccccgaca tatccagttg
     6601 caccaataat accaactttt attttattca ttttgttccc tccattttta tattcaaata
     6661 gtcttaaaat cctatctttc cactttacta tatgataatc atttgataat acgtagatta
     6721 aaatactcta attaaaacaa aaaacccctt agcattataa atactaagag gcgattttcc
     6781 gcggtaccac tcttattggt aaaattacaa taatcattaa aactcaaatt aaaaagtatc
     6841 tacaaaatgt acatttaaaa ttaaaaaagc ctcttggtca aagaccaaga gacgaatata
     6901 cacccgcgtt accactctaa ttgataagat aatcttaccc actctaatct cttaaggcga
     6961 gattcaccaa ttaccatact attacttctg gtaatctcct ccaaagctct attcacaata
     7021 aaattcgtta ctaggcttcc accattccca gctctctatc aactagcttt atgctactct
     7081 ctttttcaca ggatttcaat ttattttctt attgttttat atgatagttt atttttttaa
     7141 ctttgtcaat ataattttta agtttttgga aatattttct atataattaa tttccagcat
     7201 tttgtatcta aattgagtta taaatatcta cagtaatagg acaccatgaa tttcctgctg
     7261 taatatattt taaaaaagtt ctccctcttg aatcaagtca ttcaaaagct atacctttgt
     7321 caaaattatt tttcatccag cgttttttag aaaaaacaca attttctcca taaaagagac
     7381 atacatacaa ctattctttc ctaatgtaat atatgtatga tttctttgtc atttaacttt
     7441 ttcaaaaacg caattaacaa tggaataccg aacaacccaa taaatccaaa gaaatgtaat
     7501 cctgttagca tactggctaa ggccaataca ggatgtaact ccatatgttt accaactaat
     7561 tttggttcaa taatattcct gataatagta atcacaatat atagtagtgc aattcctaat
     7621 gccattgaat aatcccctaa aaataaactg ataatacacc aaggtattag aatacttcca
     7681 gttcctaaaa caggtagtat atctaaaaga gcaatgaata aagcaattat tccagcataa
     7741 gaaatattaa taataacaaa accgacatat aattcagaaa aagtgatagc aaaaattaaa
     7801 agataagaaa aaccacattt aataatggta tcaatagtaa acgcttttgc ttctgctaaa
     7861 agataacttt tatttttagg tatctgtagc actataaatc tctttatcgt tggataatca
     7921 attgctataa aaatagaagc aacgattgta ataattgttt tcataaatag taatggaaca
     7981 gacgaaacaa tatcagtaat accagatacg atattatttg aaagagaaac tatgaaaaca
     8041 ctcatttcat tcatcaatac aggcatattt gcttctatta attcaataaa cgaaaaattg
     8101 aatgaaccag aaaatagttc gacttctttt ataactgttt cagtcaaagg tattaccaat
     8161 tcctcaaaca tggttggtag gaaatgaaaa atttccttta ttccatatat tagatatgta
     8221 cctattacac ttactattat tcctgcaatt aaaaagaata aaaaaacaga tatggttgct
     8281 acaatacctc gctttattag taatttttca cttatgtata agactggttt attcaataaa
     8341 cttgctatta aaaatgcaaa aacaaaagga agtaatacag aaaacgcata tttccctaat
     8401 aacattagta agcctaatat cactaccaaa taacagaaat ttattaaaaa tctctgctta
     8461 ttttggtatg atgtatgatc ttgttcattc aaataaaatg cacctcctac tttatagtaa
     8521 ctttatatgt gccttttgga gcaacagggg gcattgtttc acacattcca gaacaacttt
     8581 ttgcttgcac aaaatattca atcatatcac ctgactttcc tatcatattg gcaaaaaata
     8641 tttctgcttc tgtagtttct tctaacctaa tactttccca tgtttgaaaa ccatagtatc
     8701 gataaaacag tctacaattt tcataatcta agccagcttt actataatca actatttgaa
     8761 tagtaacttc taaagcatca ttctctgaaa cgatttcttc taatcttttc acggaaatat
     8821 agagcatatt ctcatcccaa attgctcttg tcctgcaatg aataacatct tcatttcccc
     8881 agccagttct cacatatcct gtacgatttt ttataatatc tggctctttt tcaaaatcaa
     8941 actcatatcc tttaatttca tatccaggca aagcgttctg ccattgtttt attgcaattt
     9001 tatcctgttc aattccatac attggaacat agacacgact atttaaaatc acagaattaa
     9061 cataagcagt aagttcatca ctgtcctttt gaaacggaac aatatctata cgaaagattt
     9121 tgaatggacg tccatatctg ctttctacct tttttacttc ttctaaaatt ttttcatatc
     9181 tttcatacaa atagtgttct tttggcgttc tgctcacaag taatgtttct tcatcaatta
     9241 tttttaaata acagtcaata tgttgtaaac cataattagc aaaattagaa aatatataat
     9301 actcattcat tccagtatct cttgccacca tagaaaaaaa ttcttcgtca gaaattcctt
     9361 tcgcacgatt ttctgtctgt aaaatgtgca tagacatcaa tttattgaaa ccatctgaca
     9421 taagatttcc gcctgttagt gcataaggta atttcataaa tggcaaacca atctgtttcg
     9481 caatcaactc ttgtgattga tcttcttttt gttcataaga gttagtagtt aataggttat
     9541 gttcgcaatc aaacatgatt tctggtttat ttatggatac aaatggtgtt gacatcttat
     9601 attctgcacc agccaaaaag agttttccgt tttcatcaaa aatagggtgc ataccccaat
     9661 cacgaaccca tggtgtatca aatccctgtg gagcaatgca gaaaatcaaa ttttttaaat
     9721 ttccacccca cttacttaaa taatattttg caatttcaat ttcctcttta tttggtacac
     9781 acatgtatac agaataattt tgcgttaaat ctacaatata cttatgggga agaaaaggtg
     9841 gccatgcaag catgacacct ttaatcggtt cccattcccc agctaaacgt cttttcattt
     9901 ctaatacctc actttcttac tttttccaca aatgaaatgg aatttctcta attttaagat
     9961 ttttcaacaa tgtttcaaat tcattttttt gaaaggtttt ctgattacat aaaacttcca
    10021 tttctagtcc cttatactcc atgagatatt ctgtttcata aaacccattc tgaagataaa
    10081 attgcttcct taaaattctt tgctgtaaat taggagcact gtcctctata gtttccatat
    10141 caagaacaat ttgacaattg ggatattgtt ttttcataag tgacagtgct tttccaccat
    10201 atcctttaga gcgttgttct ggggaaattg caagaaataa tatatatcct atatgatttt
    10261 caatcatcaa aacataaaaa ccaataaatt gttgcttatc atataaagca gatacttcta
    10321 tttttttact ttgaactaaa ttaagcattt cttgtataga catacgttct tcatctggaa
    10381 atgcctcgtt attaagttct tcaaattttt ctaaatcttt aaaatatgaa gttacttttt
    10441 gtattgttaa gctcatatag atacctccaa attgatttaa taatgatatt ttttctgttt
    10501 tcttattata aagaaagccg tcataatagt tgccaatatt tcagcaccaa caatagaaaa
    10561 ccatatccca tttactcccc agaatatagg gaatataagt actgatacaa cttgaaatac
    10621 taaaatacgc ataaaagcaa taagcgcaga agttaaaccg tcatttaatg cagtaaagaa
    10681 actagaacca aaaacaccaa aaccagaaaa taaaaaggaa aaagagtaaa tcataaatgc
    10741 tcttaatgtc atttccatta aattattatc gtaacccaca aaaataaaag caatcggttt
    10801 tgataaaaat tcgcctatta taaacataaa tacagcaaaa caaataatta caatcaaact
    10861 tctttttctt aaatttttta attcgtcata attttttgct ccataatgat aactgattat
    10921 aggggcagta ccaacagaat acccaataaa aacagcttga aatagtaacg ctacataaag
    10981 aagaacacca taggatgcaa cgccatcttc gccagcatat tttagtaatt gagcattata
    11041 cagcatactc acaacagatg tggaaacatt agtcattagt tctgaagaac catttgcaac
    11101 agtctttttc aaaacatcac aatcaaattt aggtttctta aatttaagca aactgttgtt
    11161 ttgtcggcta aaataaagta atggaatgat accaccaaca tattgaccga ttgcagttgc
    11221 cagtgctgca cctaccaatc cccatttaaa aacagtgata aataatgcgt ctaaaacaat
    11281 atttgtaatt ccagccataa ttgcaacata taatccaagt tttggctttt ctgctgttgc
    11341 aaataaacac tgaaattcgc actgcaaaat ataggcagga atagcgataa ttaccacaca
    11401 tccataaaga atactatctg tcaataattg accatctgct cccatagcag aagcaatcca
    11461 tggcatagtt actataccta aaacagctag aataatacca caaccagcag aagtaacaat
    11521 gagtaacgaa aactgttcgt ttgcttcttc tttttttcct tcacccattg ttttagcaat
    11581 taaagctcca ccacctgttc caaaaagaaa gccaacacac cctaagatta gcaaaaatgg
    11641 cataataaaa ttgacagcag taaaagcagt tttccctaca aaatttgaaa caaaatatcc
    11701 atcaacaacc ccataaatag aacttattac cagcataata attgtaggaa atgtaaattt
    11761 taataagcgt ttataattaa aattatctga caactgtatc attgttcata cctcctaaac
    11821 caatactctg aaaagttagc aattgcataa gaaagatgtt ttatattcca ttcagatgta
    11881 ttaactataa tatgatagga agtttgattt ccccataagg agctcgtaaa aaattctctt
    11941 gatcttgctc gatttttatc aatctctctt atctttcttt ctaattcttt ttttgaaata
    12001 tgttcattgt ttaacttttt ttcaatacag cgttgtattt taaagttcat atttgcacaa
    12061 acaaaaacgt taaatggctg gtacttcttt aataatatat ctgcatttct accaacaata
    12121 acacaatcct tacctgtact ggcaatctga ttgataacat ttgattgttc tattaacaaa
    12181 tctgtcttca tggattgcat agacaaaata ttagtaaaag aagtacacat tgttaaagga
    12241 acgtttctcc aaccatgatt ttctaatatg taggaaacat agttttcgtc aatttttttc
    12301 tttctagcta tagcagtgat aatttcttta tcgtagtagt caaaatttaa aatatcagca
    12361 agttgttttc caagttctcg tccaccactt ccaaactctc ggctaattgt aataattttc
    12421 ataatatctt ttcctcacat ttcattgaat ataaaagctt tctatgttat cctaaagtat
    12481 ctggtaaccc taaagtcaat aggagattta aaaatgatta aaaaattata taagacaagt
    12541 gaatttgcag aattgtgtgg ggtaaaaaaa catacacttt accattatga tgaaattgaa
    12601 ttattaaaac cgagtattac ccatgaaaat ggctatcggt attatacaat tgaccaattt
    12661 gaaaaattta atattatttc tgtgctaaaa aaagcaggta cacctttaga agaaataaaa
    12721 caatatctca atgatttaga taccaatacc tttcttaatg tacttcacag aaagctaatg
    12781 gatcttcaag aagaagtgaa gaagattgaa aaaatgtgtt ctatgttgag aaatgtcatt
    12841 tcaacaatgg aaatacatct caatgtaaac acagaaaaaa tcgaaattgt aaattgtgag
    12901 gaagaatatc tgattgcaac taaaattcct gaagctgtac taacagatga aaaaacaatg
    12961 ttacaaggta ttggaaatca cttacaatat tgtttattac atgattataa ttttcatgta
    13021 ggagaaattg ttcttgaaaa aaatataaaa cagggaattt ttagagagag ctattattat
    13081 agccttatta aaaataaagt agatgatgaa cgccttttta ttaaaccaaa gggaaattat
    13141 gcagttatgt atcaccaggg gagttatgac agtttatata aaacttatca aaatatgaaa
    13201 aatcagttag aagaacaagg gtatcatatt gttggaaata tttatgaaga agatttaatt
    13261 gattatttat cagaacataa cgccgaaaac tacattcaaa aaatttctat gcaggttatt
    13321 tgaaaatata aaaagcgtgt atatctcaaa tcacatgaga aatacacgcc ttttttaata
    13381 tctcgtattg tctataataa tttgcttaaa atgtcctttt ttatcttcta aagttggtat
    13441 gttatttcca aatttttgta gaaattcagg ttcattgcta ttatttgttt cctcaatgca
    13501 aactgcattt cctcatgaat ttcagataca gaaatagctt tttattttcg ttaaccattt
    13561 aatcagtttt ttactatctt tttataaaat cttaacatgg aggctttatg cctccatttc
    13621 ttttgtagtt gtcttaaaag tagtgcaaat ttttttctta aatatatatt atataaatta
    13681 ttcatttata tagagtatta tattatgctc tgtaaatagt tacaatattt aaaagaatga
    13741 aaggagaaca aaaactatgg aaattgatta taaagcaatc ggacagcgaa ttaaaattgc
    13801 aagaattaaa aaaggtataa cacaagaatc cgttgcaaac attatcaaca tgacaccatc
    13861 ccatatgagc aacgtggaaa caggaaaaac gaaagtcagc ctgccaacct tgattgcgat
    13921 tgccaatgca ctttctgtat cagtcgatac tctcttatgt gataatgtac tttcttccaa
    13981 agtaattttt gaaaaggaag ccaaagatat tttctcagat tgtgatgaat atgaaatccg
    14041 ttctttagtt gaactgctaa aatcagcaaa aactgtaatc cgtaaagaca gagaaatccg
    14101 caaacaattt gaaaaataaa accacagctt caaggtataa attgatatgt gttgttagct
    14161 aaggtctttt ctttttttat ataatcattt ttatataatc aaataaccta tccaataatt
    14221 ttccaattac tgtccttatg aaatatcaat ttatactatg atacctgtgt cgcttttgtc
    14281 tgattatcaa ggaattttac agctaccttt tcattatcaa cgacaacagt aaaaataggg
    14341 tttaccaatt cagaaagtaa atattcttga aatatactat tttcctttca taaaaatgat
    14401 aaaattttat tttacatccc acgtaatgaa gtgttcaata aataagttaa aataatttat
    14461 ttacctcctt aatcatattt tttatgaata cctaataaca cagtatcaat agaatcgact
    14521 agacctttta tagcttcttc tttactcaaa agtgatttcc caagttgcaa agtcgaataa
    14581 ccatgaagta cactcattat taagtttaat acttcacctg tattctttgt atttagatta
    14641 caagaaagaa taagtttttc taataaggat ttatattcat caaagatttc taatgtttca
    14701 ttatttccat gccaagtagc ccactgaatt gtttcataaa caccaggatt agaaattata
    14761 aaattaaagt atgctatact tatacacttt atagctgtat ctccaaaatt tccaatagcc
    14821 gcctttatca tttgactatt catctctctc attcctttat gagctacttc aagtaataaa
    14881 tcatctagac tttctatatg attgtacaaa gatggtgtac gtattcctag cttttctgca
    14941 ataactttta aagaaacttt gttaagccca tctttattaa ctatatctga tgtagcttgt
    15001 ataactattt ctctactaac acgcatgatt ttccctcttt catttaaaat taattacttt
    15061 attagtttaa ctaatctaat tagtttcgtc aattattgta atttataaaa ttctttatac
    15121 tattataaaa tcttgacagt ttaattataa tttaatataa tactaattga attagtttta
    15181 atactaatat aattagtatt atattaaatt ctatattaca acattatttt atagtttgat
    15241 taatttaaca atcactaata tatcaaactg aaaggagatt tttatggaaa aaaaatattt
    15301 cgaaattcat ggagttcctt caatcctttg gggaaatcct tcaagtaaat tatatattta
    15361 catacatgga caatgtggca ataaagagga ggctgaagct tttgctaata ttgcagttcc
    15421 acatgagtgt caagttttaa gtgttgattt gcctgaacat ggagaacgta aaagagaaaa
    15481 aaattcattt aacccatggc atgttgttcc agaattagta tctattatgg aatatgcaaa
    15541 gtgccactgg aatcaaattt cgttatatgc aaatagcatt ggagcatggt ttagtatgtt
    15601 aagctttgaa aaagagaact taagagagtg tatttttgta tctccaattc tagatatgca
    15661 acagctcatt tcaaatatga tgctttgggc aaatgtatca gaagaacagc ttaaacatga
    15721 actaattatc ccaacttcat ttgggcatac tctttcatgg gaatatctac aatatgtaaa
    15781 acaacatcct atcactcaat ggaatgtacc aacaaaaata ttatatggta aacatgatga
    15841 acttactgaa atcggtgttg tagagaaatt tgtagaaaag tttaattgca accttactat
    15901 tatagaaaat ggcaaacatt ggtttaacac accacaagaa ttaaattcat tatatgaatg
    15961 gttaaatact ttagttaaat aaaaaataat tatattatta atatgattaa attatttata
    16021 aatgttattt aattaatttt attttagcat tattaaaata actacataaa gaataaatta
    16081 gttattatta aagaaattaa aagtatatag agtattaata attttttaat aattagttat
    16141 tatagaaaaa a
//
LOCUS       DAGSHT010000069        15783 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGSHT010000069 DAGSHT010000000
VERSION     DAGSHT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.071"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     regulatory      286..385
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            516..806
                     /locus_tag="MJB72_001962"
     CDS             516..806
                     /locus_tag="MJB72_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBY8263070.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     gene            781..1551
                     /locus_tag="MJB72_001963"
     CDS             781..1551
                     /locus_tag="MJB72_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263071.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            1573..2586
                     /locus_tag="MJB72_001964"
     CDS             1573..2586
                     /locus_tag="MJB72_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8263072.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            2555..3289
                     /locus_tag="MJB72_001965"
     CDS             2555..3289
                     /locus_tag="MJB72_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263073.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            3450..6293
                     /gene="mutS"
                     /locus_tag="MJB72_001966"
     CDS             3450..6293
                     /gene="mutS"
                     /locus_tag="MJB72_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBY8263074.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            6304..8271
                     /gene="mutL"
                     /locus_tag="MJB72_001967"
     CDS             6304..8271
                     /gene="mutL"
                     /locus_tag="MJB72_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBY8263075.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIGVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            8309..9244
                     /gene="miaA"
                     /locus_tag="MJB72_001968"
     CDS             8309..9244
                     /gene="miaA"
                     /locus_tag="MJB72_001968"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBY8263076.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDINAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            9358..9618
                     /gene="hfq"
                     /locus_tag="MJB72_001969"
     CDS             9358..9618
                     /gene="hfq"
                     /locus_tag="MJB72_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBY8263077.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            9745..11025
                     /locus_tag="MJB72_001970"
     CDS             9745..11025
                     /locus_tag="MJB72_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBY8263078.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            11094..12440
                     /gene="pepV"
                     /locus_tag="MJB72_001971"
     CDS             11094..12440
                     /gene="pepV"
                     /locus_tag="MJB72_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBY8263079.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            12573..13652
                     /locus_tag="MJB72_001972"
     CDS             12573..13652
                     /locus_tag="MJB72_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBY8263080.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            13742..14374
                     /locus_tag="MJB72_001973"
     CDS             13742..14374
                     /locus_tag="MJB72_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBY8263081.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            14418..14951
                     /locus_tag="MJB72_001974"
     CDS             14418..14951
                     /locus_tag="MJB72_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBY8263082.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            15000..15686
                     /locus_tag="MJB72_001975"
     CDS             15000..15686
                     /locus_tag="MJB72_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBY8263083.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
BASE COUNT         6475 a         1640 c         2753 g         4915 t
ORIGIN      
        1 tgtaatctgt aacgattgca aattagatga gtgaaaacag aaatttagca caatatttgt
       61 atggaattga tatacatcca aatatgcttg aaatagcaaa ataaaaacta gaaaattcag
      121 ttaatctagt tttggaagat gttgataaaa taccatttta agataattta tttggtgtaa
      181 taatgtacaa tttatattag atatttttga acggataaaa gatctaatat aaataaaaaa
      241 tttgttaaaa tatgataagt gtagtataat caagtagaaa agatataata tatttcgggg
      301 agctaatgtg ctgagaggag attttatctc gaccctttga acctgtttgg ataatgccag
      361 tgtagggaga ccagttaaga taaaaataag tcttatccta ttttggataa gacttttctt
      421 ttatttaaat ataatatctt tttagtaata aaatttttta ataaaaaaca aaatatacct
      481 agtaggtatt aattattttt aaaggagtga tttaaatggt aatcgcagac atagcagtaa
      541 tgccactaag accttacaaa ggtgaagaag aaatgtataa agtagtagat gcttgtattg
      601 ctgaaattga aaaaagtggt cttaaatgtg aaataggagc aatgagcact actgttgaag
      661 gtgattttga tgaagttttt gaattattaa aaaaggtaca taaaatccct tttaatcttg
      721 gatgtgaaag agtcataaca gtggctagag tagatgaaaa agctggaggt ctgactatag
      781 atgaaaaact tagaaatcat agataaagtc aaagatagta tatatccaat agcaacattt
      841 ttgtttatac ttattctttg gcaatctatg gtaggagttt tagaagttcc tcagtatata
      901 ctacccacac cagtagatat aattaatgtg ttttttaaag attatcaaaa tttatccatg
      961 catgctgtgg ttactatagg agaagcaata cttggtttta tagtcgcaat tataatatca
     1021 ttggtaattg gaatattgat ggattttgta tcaataataa aaaaatgtct ttatcctatt
     1081 atgttggtta cacagatgat acctacaata attatagcac cactctttat gatatggttt
     1141 ggatttggaa caatgccaaa agtattgatg gtaatgctta cttgtttttt tcctatatta
     1201 ataagttttg tagatggcat tgagaatatt gataaagatt atttaaattt atttaagact
     1261 atggactcaa ataaagttaa tacattcatt caccttaaat ttcctatggc aatggataaa
     1321 tttttttctg gacttaaaat atctgcaacg tatgctgtaa tggcagcaac tgttgcagaa
     1381 tggctaggtg gaacgaaagg acttggtgtt tatatgctta ggtcaaaaag tgcatatgca
     1441 cttgataaag tatttgcatc tactatatta gtagttatat ttagtttaat gtttgtagga
     1501 attgtacagg ctgtaaaaaa agttgttata agacatagat taatagatta aaggtatttg
     1561 agggaggcaa ttatgaattt taaaaagtta ggaatattag gtttagtaag tgtttttaca
     1621 ttggcaacaa taactggatg ttcttcaaat aacaataaag acaaaaacaa tgataaagat
     1681 acttcttcta aaaaagtaac tatggtgctt gattggacac caaatacaaa tcatactgga
     1741 ttatttgtag cacttgataa gggttactat aaagaagaag gtttggatgt tgaaatagta
     1801 caaccacctg aaagtggagc agaaacttta gtagcaacag gaaaagctga ctttggtata
     1861 agctatcaag aacaagttac ttatgcaaag actagtgaag atccacttcc aataaaagct
     1921 gtagcaactg ttatacaaca taatacttct gggtttgcat caccaaaaga aaagaatata
     1981 acaacagcta aagattttga aggaaaaact tatggtggtt ggggctctcc atcagaagaa
     2041 gctgttttta aagcagttat gaagaaaaat ggagctgatt ttaataagtt aaagatagtt
     2101 aatactggtc aagatgattt ctttgcagct atgaaaactg tagattttgc atggatattt
     2161 gaaggttggg atgcagttaa agctgattta attggatatg acttaaactt tataccagta
     2221 aaagatttag atgagagatt ggattattat acaccactta taatttctaa tgaaactgtg
     2281 cttaaaaata atccagagct tgctaaaaaa ttcttaaaag ctacaacaaa aggttatgaa
     2341 tatgcaataa aaaatccaga agaatcagca aaaattttag taaaacatgc acctgaagta
     2401 gatgaaaaac tagctttaaa gagtcaagaa tatttagcaa gcaagtacaa agatgatgca
     2461 ccacgatggg gagaaatgaa agatagtgta tggaacaact atacatcatt cttaaaagaa
     2521 tataaattaa tagataagga tatgaaggca agtgatgctt acacaaatga atttttacca
     2581 caataaaaaa gtaaaagtat ctatagaaaa tgtatcaaaa acttataaag atattgaagt
     2641 tttaaaagat ataagtattg atgtttatga aggtgagttt gtttctatat tagggccaag
     2701 tggatgtgga aaaagtacta tttttaatat tataactaaa cttacagatt atgatagtgg
     2761 caaagtgaat ataaatggta agtatagtta tatgtatcaa aaagatttac tcttgccata
     2821 taaaactata atagataatg tgtcactacc actaatttta aaaaaagaaa aaaagagtaa
     2881 agctaggtca atggtaaaac cttattttga ggtgtttgga ttaagcggat atgaagataa
     2941 atatccttca gaactttctg gaggaatgag acagagggct aacttcttga gaacttttat
     3001 aaattctaat gacataatgc ttttagatga gccttttggg gcacttgatt cattaactaa
     3061 aacatccatg caaaaatggc ttttagatgt taagaaaaaa gtaaattcaa caatacttct
     3121 tataactcat gatattgacg aagctataat gctatcaaac agaatttatg ttatttcaaa
     3181 aaaaccatca atagtaaaga aagaatttgt tatagatagt agaaagttaa atgaagataa
     3241 ccttgaaaat atcattaaac ttaaaaaaga aataatatct ttattgtaaa aaaaaatatt
     3301 tttatttaat aagcattaaa cttagcaatc caaaatgtga gtatacattt tggattgttt
     3361 ttagttatat gtcaaaattt tatatattgt gttaataata atcatctact atgatataat
     3421 atttaattag aaaagaagga ggtttgataa tgcaaataga tatgaataaa ttaactccta
     3481 tgatgaaaca gtatcttgaa gtaaaaaata gatataaaga ctgtatactg ttttttagac
     3541 ttggagattt ttatgaaatg ttttttgaag atgcattagt agcttcaaaa gctcttgaaa
     3601 tagcattgac aggaaaagct tgtggtttag aagaacgtgc tcctatgtgt ggtgttcctt
     3661 tccactcagc caattcatat atatcaaaat tagttgaaaa tggatataaa gtagcaatag
     3721 gtgaacaaat ggaagaccca tctacagcaa aaggaattgt tagaagagag gttataagag
     3781 tcatcactcc tggtacagtt ttagatggaa atttacttga aaataaaaag aataattatt
     3841 tattatcttt gtataaggat ggtactaata taggtctaac ttatgttgat ataagtacag
     3901 gagaaactaa tgcaacatgt ttaaatgaag ataaggtaat tgaagaaatt gcaaaaatcc
     3961 atccaactga aataattata aatgatttag actttataga aaagcttaga gatatagcta
     4021 ctgtaagtaa tatatacata aatgaaagtt ttagtgataa ctatttggat ataaatatat
     4081 taaaagaata ttttccagat gtttatctac aaaaactaaa gtttgatgat aaaggtctaa
     4141 taaagtcaag cttatcaatt cttctgaatt atatatacaa tactcaaaaa caaataacat
     4201 ctaacattaa taatataaat atatacaatt catcagaata tatggtttta gatatgttta
     4261 ctagaactaa tttagaactt actcaaacta taagagggaa taagaaaaaa ggttcattat
     4321 tacacgtact tgataaaact tctacagcaa tgggaggtag acttttaaga aaatatgtag
     4381 aagaaccttt gataaataaa tctaaaattg aaaatagatt agatgtaata gaagaaataa
     4441 aagatgactt tatattaaga gaagacctaa atgatatact taaaaatata tacgatatag
     4501 aaagaatatg tggaaagata gcatttgaga gagtaacacc aaaagagtta attcacttaa
     4561 aaaattcgat agaaaagtta cctaatttaa aagatactat aaatttatca aatgcaaaaa
     4621 tcctaaaaga gtatgtttca gaaatggata aattagatga tatatacaat ttaatagatg
     4681 aagctattct agaagaacct acgataacca taaaagatgg aaacataatt aaatcagatt
     4741 ttagtgatga acttaaggaa ctaagagaga tttctaagaa tggagccttt cttgtaaaag
     4801 aaatagaaaa cagggaaaga gaaaaaacag gagttaaatc attaaaaata ggatttaata
     4861 aagtttttgg gtattatata gaaataacaa aagccaactt taaacaggca aagctagatg
     4921 agacatatat aagaaagcaa acacttagta atgcagaaag atatataact ccagaattaa
     4981 aggaaataga agaaaagata ctacatgcag aggaaaagat taaatcttta gaatacgaga
     5041 tatttgtaga gataagagat acaatatata aaaacataga tagaatacaa aaagtggcta
     5101 agactatagc taatatagat gtttttgtgt cccttgcaac agttgcacat ataaataact
     5161 atgtaaaacc agcaataaat gaaaataata aacttgatat aagaaatggt agacatcctg
     5221 ttgtcgaaaa tatagtagga gaggaaaatt tcgttccaaa tgatacttat ttaaatcgtg
     5281 gagaaaacat tattaacata ataactggtc ctaatatgtc aggtaaatca acatatatga
     5341 gacaaactgc tataatagct cttatggcac atataggctc atttgtacca gcagagtctg
     5401 ctgatattcc aatattagat agaatattta caagagttgg agctagtgac gatttatctc
     5461 aaggtcaatc tacttttatg gtagagatga acgaagtatc ccttatatta aaaaatgcaa
     5521 cagagagaag tttagttata ttagatgaaa taggtcgtgg aactagtacc tatgatggta
     5581 taagtttagc atggtcaata gtggaatata ttcaaaaaaa tattagatgt aagacattat
     5641 ttgcaacaca ttatcatgaa cttactgatt tagaagaaga gtttaaagag gttaaaaatt
     5701 attcaatagc agtcaaagaa gatggggaag gaataatatt tttaagaaaa ataattcctc
     5761 aaggagcaga caagagttat ggaatatatg ttgctaaatt agctaaatta ccagacgaag
     5821 tcatagaaag agcaaaatat atacttaaag acttggaaaa aaatcatgtt tacaacagtg
     5881 ttgcaataaa tggagataaa gaaaacaata atataattaa tagtaatttg gacgaagaat
     5941 tagttaaggt aaatcaaagt aattttaaga ataaatatga atctctaaaa atagaacatg
     6001 aacttatagt aaaagactac aaacatataa aaaaggatta tgataaatta aattctaaat
     6061 ttaaagcatt aaatgatgaa gttgctttaa tgaagcaaaa tgatgataaa gagaaaatta
     6121 atcaagaaga ttctgtaaaa gaagtagcct taactcaaat ctcttttgat agtgtaaata
     6181 gagatatact atcagaagaa attttaaatc tggatatttt aaatatgact ccattagatg
     6241 ctataaactc tctttataat cttcaaagaa aagcaaaaga aataaaatct tagaggtgta
     6301 aaaatgaaaa atataattaa catcctagat gatttgacta taaataagat agcagcaggt
     6361 gaagttgtag aaagaccttc ttctgtagta aaagaactta ttgaaaactc cattgatgca
     6421 ggtgcaaata aaatttccat agatataatt gatggaggaa aaagtctaat taagattaca
     6481 gataatggaa taggaatacc ttcaagtgaa gttgaaaaat catttttgag acatgctact
     6541 agtaaaatta aaaaaattga tgatttgtat gatttatact ctcttggttt tagaggagaa
     6601 gcattggcat ctatttcagc tgtatcaaaa ttagaaatga cgactaaaac taaagatgaa
     6661 ataataggaa ctaaaatata tgttgaaggt ggaaaaataa tatcgaaaga accaataggt
     6721 tctactaatg gaactaccat cattataaaa gatattttct ttaatacacc agcaagacag
     6781 aaatttttaa aatctactca tgcagaaact ataaacataa gtgatttgat aaataaactt
     6841 gcaataggta atccaaacat tcaatttaaa tatacaaata acaataaaca gatgctaaat
     6901 actccagggg atggaaaact tgtaaacact attagaagta tatatggaaa agaaataacg
     6961 gaaaatataa tagatgtaga gtttaagtgc aatcacttta aaatgaatgg atatataggt
     7021 aataacaata tataccgttc aaataaaaac ttacaacata tctatataaa taaaagattt
     7081 gtaaaaagta aaataatcat agatgcaata acagaaagtt ataagtctat aattccaata
     7141 ggtaaacatg cagtgtgctt cttaaatata gaagtagacc catcatgtat agatgtaaat
     7201 atacacccca ataaacttga aattaaattt gaaaaagaac aagaagtgta tatagaactt
     7261 agagattttt taaaggttaa attaatacac tcaaatctta ttggaaaata tgctacttat
     7321 agtgataaaa aaacacaacc tagaatcgct attaacagta gagaaaaaag tacagattat
     7381 aaattaagaa ataatgattt attagaatct actcctaaaa atagtaatat tactaaaggt
     7441 aaggatgaag tcattgaagt tgttacttta tcaagtgaaa aaccaataaa tgaatttcaa
     7501 tcagtaagtg aagtcctaaa tgcaagtgta gaagatgatg taaaaaacat taattatcta
     7561 agtgaagata gtgcaaatga taatatacaa gaagagtttc aagttgatgg aattaaaaat
     7621 gaaggaaatt attacttagg agatagtatt aaggacagtg aagaagaata cttgtgtagt
     7681 tcaaaacgca agttttcttt atacggatat tctgtaattg gagttgtatt taatacttat
     7741 ataattttga gcaaagacga ttctatgtat ttgttggacc aacatgctgc acatgaaaga
     7801 atactatatg aaagatatat ggagaagttc tatagacaag atataaatat gcaaatactt
     7861 ttagacccag tggttattgg agtatcaaat gtagatatgc ttcaaattga aaataacttg
     7921 gagttattta tgaaatttgg ttttgagcta gaaatttttg gcaataatca tataatggta
     7981 agatgtgttc ctactatatt tggagtacct gaaactgaga aatttatact tcaaataatt
     8041 gataatatag aagaaattac aagtaattat gatttaaaag gagagagatt tgcatctatg
     8101 gcatgtaggt ctgctataaa agcaaatgat aaaatatacg atattgaaat aaaaagttta
     8161 ttggaacagc tagaaaagtg tgagaatcca tttacatgtc ctcatggaag acctataatg
     8221 gtcgaaatat ctaagacaga aatagaaaaa atgtttaaaa gaattatgta aaatatttgg
     8281 atatttaaaa tatatggaaa ggaatgttat gaaaaaaata ccacttataa tacttacagg
     8341 tccaactgct gttggaaaaa cagatttgtc aataaaatta gcaaaagata ttaatgcaga
     8401 gataatttca gctgattcta tgcaaatata tgaatatatg gatattggaa gtgcaaaagt
     8461 tacagaggaa gagatgcaag gagtgaagca ttaccttata gatgaagtta agcctgacta
     8521 tccattttca gtttcagagt ttcaacaaag agcaaaaaaa tatatacatg aaataaatga
     8581 aaagggaaaa tgtgttttag taactggagg cacaggactt tatttaaact ctttaatata
     8641 taatatggat tttgcacaat ctgatgccaa taatgaatta agagaagaac ttcaaaaaca
     8701 gcttgctgaa aagggaatag attacatgca taataagtta aaagaacttg atgaagaatc
     8761 tgctaataga atccataaaa acaatactaa aagggtcata agagctcttg aagtttgtct
     8821 aagtggtaag aaaatgaatg atttttcaag tgatttaaaa tttaatgaag aatatcaacc
     8881 aataataata gttctaaata gagatagaga acatttgtat caaagaataa atatgagagt
     8941 tgatattatg attaaaaatg gattggttga agaggtaaaa aaacttttaa gtatgggatt
     9001 taaaaaagat atgatatcaa tgcaaggtat aggttataaa gagatattaa agtatctgga
     9061 tggagaatat acatacgaag aagctattga aataattaaa agagattcta gaaggtatgc
     9121 aaaacgtcag ataacatggt ttaaaagata taaaacagca aaatggtttg atttagacca
     9181 atatgaaaat atagacgaat taaaaaatga aataatctta tatattaaag attctataaa
     9241 ataatatata aatttttaag tatacaactt aatattgaaa atttgtcata acttatatag
     9301 aatataaggt aggaatattt tagaagttca atttaatctt gggagggtac aaatctaatg
     9361 aaaaatacag ttttaaattt acaagatttg tttttaaata atgcaaggaa agaaaggata
     9421 cctgttacta tatatttagt taatggagtg caagttaaag ggcttgtaaa ggggtttgat
     9481 agttatataa tattaataga aggggataat agacaacaaa acatgattta taaacatgct
     9541 gtatcaacta tacaacctgg aaagtatatt aatctaacaa atcaaaacca aaacaacaat
     9601 aataataaca acagatagat aattaattta atttaagatg attgagagga gacttatact
     9661 aacttataaa ctataattta tttgttttag taaaatagtc tcctttacat gtttaaataa
     9721 aataataaag agacaggtga taaaatgctt aatgagacaa aagaattatt aaaagactac
     9781 tatggaatag atgatgatac ttttaaacta tcgcaagaaa taatggaaga aataaaagat
     9841 aagtttgaag aaataaaaga aataagagaa tataatcaat ataaagtttt aaaagctatg
     9901 caagaatcta aactaagtga tatgcacttt aactggacta caggatatgg atataatgat
     9961 attggtcgtg aaaaaataga ggaaatttat tctaaagtat ttaatacaga agatgctctt
    10021 gtaagaccaa taattgtaaa tggtacacat gcacttactt tatgtataca aggtatagtt
    10081 agaccaggag atgaaatttt atctgttact ggtagaccat atgatacttt agagggagtt
    10141 ataggcataa gagaagaaaa aggttcattg aaagagtatg gtgttacata tgatgatgta
    10201 gattttttag aggatggaaa cttagattta gaaggaatta aaaacaaaat aaatgataga
    10261 actaaactcg ttatgataca aaggtcaaaa ggatattctt ggagaaaatc actttcaatt
    10321 agtgatataa aagaagctat agaagtaata aagtctgtta aaccagaagc tatagtaatg
    10381 gttgataatt gttatggtga gtttttagat accaaagagc ctactgatgt tggagcagat
    10441 gttatggctg gctctttaat aaaaaatcct ggtggtgggc ttgcattgac aggtggatat
    10501 atagctggta gaaaagattt gattgagctt atatcttata gaatgacttc tcctggaata
    10561 ggaaaagaat gtggtcttac ttttggaaca actagaaatg tacttcaggg ttttttcttg
    10621 gcgccttata tagtatctca ggctgtaatg ggagctatct tttgctcaag agcttttgaa
    10681 aaattaggat atgatgtatt accaaaatat gatgacttaa gaagtgatat tattcaatgt
    10741 attagactta ataatgctga tgaagtaata agtttttgtg aaggtataca agaagctgca
    10801 ccagtcgatt cttatgtaaa acctgttcct tgggacatgc ctggatacga aagtgaagtc
    10861 ataatggcag caggagcatt tatacaaggt tcttctatag aattaagtgc agatgctcca
    10921 ataagacctc cttataatgt ttattttcaa ggtggactta catttgacca ttcaaaaatg
    10981 gggactttaa aagcgattga atttataaaa aaactgaaaa aataagcata ttttttttta
    11041 aaaaggtcaa aatatttatt agtatattat gatgaaaatt gaagggtgat aatatgaaag
    11101 agcaaattaa agaaaaagta aattctctac aagatgaaat gatttcttca atacaagaaa
    11161 gtgtaaaaat acctagtgtt atatctgaag caactgaaaa ttgtccattt ggtgaaaatg
    11221 tagataaagc tttaagaggt atattagact tatgtaaatc tttaggattt aagacagtat
    11281 acaaagatgg atattatgga tatgcagaaa taggacaagg tgaaaaaatg ataggtatac
    11341 ttggtcacgt tgatgtggtt ccagaaggtg atttagaaag ctggaattac cctccatttg
    11401 aagctgtttt agaagatggt aaattgtatg gaagaggaac acaagatgat aaaggaccta
    11461 caatatcagc aatttatgca gtgaaagctc ttatggattt aaatgtagac tttaataaga
    11521 ggataagatt catttttggg gctgatgaag aaaatctttg gagatgtata aataaatata
    11581 aagaaaataa tgaagaaata ccaaactatg gatttactcc agattctaga ttcccaataa
    11641 caaatgcaga gaaaggtctt ttacaagttc atttgacttg tgatagcaaa agtgatatag
    11701 agttgtcagt tggaaaggct ttaaatgcag ttccaggtaa agcaatctat attggaaaat
    11761 actctgataa attaaaaaaa gaacttgata aattaaattt tgagtatact gttgagggta
    11821 ataaaatctg tataattgga aaaagtgtcc attcagcagc aagtgatact ggtataaatg
    11881 cagtggctag actttgtata gctcttaata atataggtat agattctaat ataattaagt
    11941 ttttagcaga agttattggt gaagatgcta atggaaacaa tataattcct aattgcaagg
    12001 atgatgtatc aggtaaacta acagtaaata taggtagagt aactattgat aacgaaaaag
    12061 aatttgcagg aatagatgta aggattccag taacatacaa aaaggatgat tttgtaaaag
    12121 agttaaagaa aatgacagat aaatacaatt taaattatga agaatatgat ttcttggatt
    12181 caatctatgt tccagaagat actcttcttg ttaagacact tagaaaagta tatgaagaag
    12241 aaactggtct tgatggaaca ccactttcat ctggaggagc aacatacgca agagctttag
    12301 ataattgtgt tgcatttgga gctatttttc caggaaaacc agaaacagaa caccaagcta
    12361 atgaatactt aatagtagag gatattataa aagctactca aatatatgct ctttctattt
    12421 acgaattatt aaagatttaa tataatataa ggctgtcttg taataggatt ttttctagta
    12481 aaagacagct ttattgttgt atcataaaat aaatgatatg gatatttaat ggatataaaa
    12541 ataggagtaa tatttatatt aggaggaact atatgaaaat agcaatattt ttaggagcag
    12601 gagcttcagc agcagagaat ctacctattc aaaatgaact ttttagcgaa tattttaaaa
    12661 aactaaatcc cacaaattat aataccaaaa tgaatagaga attgtatatt ttctttaaac
    12721 agatgttcaa tattgatata gtaaaagata atatagataa tgcaaatttt cctacctttg
    12781 aagaagtttt aggtctttta gacttagcag aacaaagaaa agaagcattt agaaattttg
    12841 gactagaaaa tctaaataac agaagtgata gtatacgttt tttaagacaa tatttaatac
    12901 tgctaatggc tgaagcaatt cataatacac ctagaacaga taataaatat cataaacttt
    12961 tagttgaaaa tcttttggaa aatgatttgc ttcttgatac tacttttatt agtgcaaatt
    13021 atgatataca tatagacaat acagttactg gattaagaga taagaatgat ttgccaatta
    13081 tgcttgatta tggtgtggag tttacaaatt ttaaagagga gttaaattgg agaaaaccaa
    13141 aagaaccatt aatcaaatta tataagatac atgggtcatt gaattggctt tattgcccca
    13201 tttgtaatag tgtaactctt acaccacatg aaggtggtgt catgaagctt attgaaaata
    13261 gttcagagac aaaatgttta gagtgtggtg agttaacaga acctattata gtgccaccaa
    13321 catattttaa gaatatgtca aacatatttt taagtaatgt ttggaatgaa acagaaaaaa
    13381 ctttaagaga tactgacctt ttaatatttt gtggatattc atttcctgaa gctgatatgc
    13441 acataaaata catgttaaaa agagtgcaga ctaatagaaa aaaacctcct cttaagataa
    13501 tggtttttaa taatcattca caaaaacaga gaattacttt aaagaaagaa gaaggaaggt
    13561 ataaacgttt tttgggtgaa gatgttatat ttactgataa ttctttccaa gatttttcgg
    13621 taaatccctt gaggtttata aaaaatatat agtagtatgg tatataatgg aataaaatct
    13681 gatagaaata aaatgaattt aggtatataa tagaaaataa aaacataggg ggtatataaa
    13741 gatgaaggtt tttgatttaa ctcatgtgac acataatgac atgcctgtat atgcagagcc
    13801 taatagacca gatatcaaga aagtagctat tattgaagaa aatggttatc aagaaacatt
    13861 aattagtgta ttttctcaca atggaactca tatggattca ccaagacata tgtatactaa
    13921 aggtgagacc ctagataagt tggatataga aaattttgtt ggaaaagctt atgtattgga
    13981 acttgaaaag ggaaatgaga atatagaatt agaatatcta aaaaaatatg aagatgaaat
    14041 aaaaaatagt gattttataa tatttaaatc aggttggtca aaattttggg ataaaaagca
    14101 gtactatgta ggttatccta cattaacaaa agaagctgca aactatatag ctaatactaa
    14161 tataaaaggc ataggaatag atatgctatc agtggataga tatgacacat cagtatttga
    14221 agtgcatcat attttatttg aaaaaggaaa aataatcata gaaaatctta caaatttaga
    14281 aaatgttcca gaaaaatttc tatttatagc agcaccattt aagtataatg atgctgatgg
    14341 agctccagta agagcaatag ctatagtaga ataatacctc acaatgatat tatgatgaag
    14401 tgtttaagga gaaaagtatg agtgatttaa ataataaaaa agaacaagat gaaaatgaat
    14461 ctagcatgaa tgaaactaaa atatatgata ttttggataa attaaatgtt gaatatgatg
    14521 tagtagagca tgaagctgta tatacagcag agcaattagt cattttagat ggaataacaa
    14581 aggggtgtca atgtaaaaat ttatttttaa gaaatgccaa aggaaataag tattatctta
    14641 ttgtagttaa aggagaaaaa caagttgatt taaactcact taaagataag ataggaagtt
    14701 ctagattatc gtttgcttca cctgaaagac tatacaatgt acttaaactt cttcctggaa
    14761 gtgtaaatcc tttttcatta ataaatgata cagaaagaaa agttgagctt tacatagata
    14821 aagatgtaat taaagaagat tacttaaatt ttcatcctaa cataaatact aaaactgtaa
    14881 atatatctag aaaaggtttt gaagaatttt tggaatattt aaaatatcca atacaaagta
    14941 tagatgttta ggttttaata ataggataaa tttcaattag ctaatcgagg ggagaaaaaa
    15001 tgtttggagt aatagcaaat agtctagcaa ttgcaggtgg atgtatagta ggtcttataa
    15061 taaaaggagg attacctcaa agagtaagcg atactataat gaatggaatt gctttatgtg
    15121 tattgtacat tggtatatca ggagcactag aaggaaaaaa cacattagtg acaataatat
    15181 ccgtagctgt aggtgctctg ataggagaat taatagatat tgacaaatgg gtaaataagt
    15241 tgggtgcttt tttacaaagt aaattttcta aaggaaataa aaaagattct atagcagagg
    15301 gttttatatc atctagttta ttattctgta taggagccat ggcagtagtt ggttcattag
    15361 aaagtggtct tacaggaagt catgatactt tatttgtaaa atctgttata gatggaatag
    15421 cttcaattat atttactgct tctttaggta taggtgtaat gttttctgca atatctgtat
    15481 ttttgtatga aggtattata tgtgtgggag catcattttt aaatggattt ttaagtgatc
    15541 ctgtagtcac agaaatgaca gcagctggaa gtttattaat tataggactt ggtctaaatg
    15601 tacttaaatt aacgaatata aaagtagcca atttgttacc agctatattt ataccaatat
    15661 tatttggcgt atttggaata atttaaaaat aatttaaaga gtaataacct aagatttgtc
    15721 taaatttttt agaaacttta gacaaatttt ttatttagaa ggaaaatagc ttttatcatc
    15781 aaa
//
LOCUS       DAGSHT010000070        15555 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGSHT010000070 DAGSHT010000000
VERSION     DAGSHT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.072"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..2449
                     /gene="priA"
                     /locus_tag="MJB72_001976"
     CDS             <1..2449
                     /gene="priA"
                     /locus_tag="MJB72_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBY8263084.1"
                     /translation="YTDNLFTYQIPVFLSDVIKIGHRILVPFGKGNKPTEAFVFQFTD
                     SLDEKIKIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECINLIYPKGYKLNNYK
                     VVLLGESLSGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVDKLKYIPNLNSSLYT
                     MNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKINVGSKQKEILSFLKN
                     NENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIYKSVLKSIKLNNEQQ
                     EAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGLDSIFLVPEIALTPQ
                     TIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGARSALFAPFNSLGLII
                     IDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIEEYYRAKSGEYKLIN
                     IKSRANDKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEENNQVILFLNRRGYA
                     NFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKECPECKSTYVKPFGV
                     GTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEADVLIGTQMLSKGLD
                     FENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKEGKVVLQTYDTEHYA
                     IKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLVIKNIKNMHASLIYL
                     LEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLKGIIKYICITKRDLI
                     FDKNINVSIDINPNSVL"
     gene            2467..2907
                     /gene="def"
                     /locus_tag="MJB72_001977"
     CDS             2467..2907
                     /gene="def"
                     /locus_tag="MJB72_001977"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBY8263085.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            2922..3851
                     /locus_tag="MJB72_001978"
     CDS             2922..3851
                     /locus_tag="MJB72_001978"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBY8263086.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            3861..4613
                     /locus_tag="MJB72_001979"
     CDS             3861..4613
                     /locus_tag="MJB72_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBY8263087.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            4682..5383
                     /locus_tag="MJB72_001980"
     CDS             4682..5383
                     /locus_tag="MJB72_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBY8263088.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            5445..6770
                     /gene="rsmB"
                     /locus_tag="MJB72_001981"
     CDS             5445..6770
                     /gene="rsmB"
                     /locus_tag="MJB72_001981"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBY8263089.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            6778..7809
                     /gene="rlmN"
                     /locus_tag="MJB72_001982"
     CDS             6778..7809
                     /gene="rlmN"
                     /locus_tag="MJB72_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBY8263090.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            7829..8581
                     /locus_tag="MJB72_001983"
     CDS             7829..8581
                     /locus_tag="MJB72_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBY8263091.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            8598..10613
                     /gene="pknB"
                     /locus_tag="MJB72_001984"
     CDS             8598..10613
                     /gene="pknB"
                     /locus_tag="MJB72_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBY8263092.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            10721..11458
                     /locus_tag="MJB72_001985"
     CDS             10721..11458
                     /locus_tag="MJB72_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY8263093.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            11556..12440
                     /gene="rsgA"
                     /locus_tag="MJB72_001986"
     CDS             11556..12440
                     /gene="rsgA"
                     /locus_tag="MJB72_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBY8263094.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            12445..13116
                     /locus_tag="MJB72_001987"
     CDS             12445..13116
                     /locus_tag="MJB72_001987"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBY8263095.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            13125..13766
                     /locus_tag="MJB72_001988"
     CDS             13125..13766
                     /locus_tag="MJB72_001988"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBY8263096.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            13897..14526
                     /locus_tag="MJB72_001989"
     CDS             13897..14526
                     /locus_tag="MJB72_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY8263097.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQVGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            14581..15237
                     /locus_tag="MJB72_001990"
     CDS             14581..15237
                     /locus_tag="MJB72_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY8263098.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         6280 a         1537 c         2982 g         4756 t
ORIGIN      
        1 atatacagat aatttattta catatcaaat tcctgtattt ttatctgatg taataaaaat
       61 tgggcacaga atattagtac catttggaaa aggtaataaa ccaacagaag cctttgtatt
      121 tcaatttaca gatagcttag atgaaaaaat aaaaataaaa gaaattattg atatattaga
      181 tgaaaatccg atatttagaa aagaagattt agagttggtt tattggatga aaaatagata
      241 tttgtgtact tatatagaat gtataaattt gatatatcca aagggataca aattaaataa
      301 ttataaagta gtcctattag gtgaaagttt aagtggatta aatgatattg aattgaagga
      361 aaaaatatca actttaagtg ataaaaatag agaaatagta gagaaagtga ttgatagtaa
      421 aggtaaaatt aaggttgata agttaaaata tataccaaac ttaaatagtt ctttgtacac
      481 aatgaataag aatggaatta taaaattatg ttgggaatat aaaaatcaca aaaatgaaaa
      541 gaaggtatgt tatatatctt tgagtttaga aagtgataag attgacgatt atatagagca
      601 aaataaaata aatgtgggaa gtaaacaaaa agagatatta agttttctta aaaataatga
      661 aaatgtagag ataaatgatt tattggattt attaaatgct tcaaaacaaa gtataaattc
      721 tctaagtaag aaaaagctaa taaccctaga atttaaggat tattatagag agccaaaaag
      781 tatttacaaa tccgtactaa aaagtataaa attaaacaat gagcaacaag aagcagttga
      841 tgagataaag tcgaatatgt ttgtagatga taaaaaacca tacttaattc atggagtaac
      901 tggaagtgga aaaactgaag tttacatgga aataatagaa tttgctctaa atcaaggctt
      961 agacagtata ttcttagtac ctgagatagc attaactcct caaacaatag ataggctaaa
     1021 gagtaggttt ggagatttgg ttggagtgtt ccacagtaaa ctatctgaag gagaaaaaca
     1081 tgatgtttac aaagctgtca aagcaggtaa agtaagagtg ttaattggag ctaggtcagc
     1141 tttatttgca ccttttaatt ccttaggctt aataataatt gatgaatgtc atgagtcatc
     1201 atataagtct gagaagaatc caaaatttaa tgctatagaa gttgctagat ttatggcatt
     1261 aaaaaataat ataactctta ttttaggttc agcaacgccc tcaattgaag agtattatag
     1321 ggcaaaatct ggagaataca aacttataaa tataaaaagt agagcaaatg ataagcctct
     1381 tccaaacatt gaagtggtag acatgaaaga tgagttagat aaaggaaata gaagtatatt
     1441 tagtttgaaa ttacaaaaag aaatacgtta tgcgattgaa gaaaataatc aagtaatact
     1501 atttttaaac agaagaggtt atgcaaattt tgtatcttgt agaaagtgtg gatatgtgtt
     1561 tcaatgtgaa aattgtgata tatctttaac ttatcataag aaaagcaata ctggaagatg
     1621 ccactattgt ggatatgaga aagaaattcc aaaagagtgt cctgaatgta aaagtactta
     1681 tgttaagcca tttggagttg gaactcaaaa aattgaagag gaattaaaat atatttttcc
     1741 agatataaaa actttgagaa tggataaaga tacaacttca aaaaaaggtg ctttagatga
     1801 gatactaaat aaatttaaag ataaagaagc agatgtactt attggaacac agatgttaag
     1861 taaagggctg gattttgaga atgtaacttt agtaggtatt ttgtctgcag acatgatatt
     1921 aaattttcca gactttaaaa gttttgaaac tacatttcaa ttgataactc aagtctctgg
     1981 aagagcaggg agagctgata aagagggaaa agtagtactt cagacttatg acactgaaca
     2041 ctatgctatt aagcatgcta ttgaatatga ttatgagggt ttttatgaag atgagataaa
     2101 aataagaaaa gcatttggat attctccttt taataatatg ttaagtgtag ttgttagtgg
     2161 tgaagatgaa agactagtga taaaaaatat aaaaaatatg catgcatcat tgatttattt
     2221 attagaaaaa aggggtataa atgatttagg atttatatta ggtccaaatc catgttcaat
     2281 atcaaaaata aatcaaaatt atagatggca aatactcttt aaagatgaaa atattgaaat
     2341 taatctctta aagggtataa taaaatatat atgtataaca aaacgagatt tgatatttga
     2401 taagaatatc aatgtatcta tagatattaa tccaaacagt gtattataaa tttaggagga
     2461 attataatgg ctttaagaca aatagttcaa ataggagaac cagtattaag aaaaaaatca
     2521 aaaaaggttg aaaaaattga tgaaaagata atacaattat tagatgatat ggcagaaact
     2581 atgtatgatg cagatggtgt tggactggct gcacctcaag ttggaatatt aaaaagagta
     2641 gtagtaatag atattggaga agagcttata gaacttataa atcctgaaat aatagaaaca
     2701 tctggggagc aaatagatga ggaaggatgt ttaagtgttg ttggagaagc gggaaatgtt
     2761 agaagaccaa attatgtaaa ggtcagagct ttaaatagaa atggtgaaac aatagagtta
     2821 gagggagaag aacttttagc tagagcattt tgtcatgaga tagaccactt agatggaatt
     2881 ttgtttgttg acaaaataga aaagtagtag gagttgattt gatgaaaata gtatttatgg
     2941 gaactcctga tatagcagtt ccatgtttac aaaaaataat agatgagaag tatgaaatat
     3001 tgggtgttgt aactcaacca gataaaccaa aaggaagagg taaaaaacta ggtatgagtc
     3061 cagtaaaaga actggcaatt gaaaataata tacctgtata tcaaccagta aaggctagag
     3121 ataaagaatt tatagataaa attaaatctt taaatccaga tgtaatagta gttgtagctt
     3181 ttggacagat acttccaaaa gaaatattag agattcctaa acttggatgt ataaatgttc
     3241 atgtttcttt acttccaaaa tatagaggtg cggcacctat aaattgggta ataataaatg
     3301 gtgaagaaaa gactggtgtt acaactatgt atatggatga agggctagat actggagata
     3361 tgatactaaa gacagaagta aatcttgatg aaaatataac agcaggagaa cttcatgata
     3421 aaatgatgaa tataggcgca gaaactttaa aagaaacatt aagattaatt gaagaaggaa
     3481 atgctccaag agaagtacaa aatcatgaag aattttccta tgcgccaata atgaataaat
     3541 cattaggtaa catagatttt tcaaaaagtg ccagagaaat acacaattta gttagaggag
     3601 taaatccatg gccaagtgcc tatactacct ataatgatgt cataatgaaa gtttggaaaa
     3661 ctaaagtgtt agatgaaaaa agcacaaaag atgtaggtac aataattgat gtaagcaaag
     3721 atgggataaa agtaagtact atagataatg tacttttaat tgaagaaatt cagatgccga
     3781 ataaaaagag aatgttggtt ggtgaatata taaaagggaa tactatagaa actggtttag
     3841 tattgggtta ggtgaaaagt atgacagata taaagaagta tttaacaagt gttgggattt
     3901 tgattttaat attaggaatt ttgataggag ttgttaattt ttttataata agtttaactc
     3961 aaatattctc cttgatgata aatattataa taatatcact aatacttata atactatgtt
     4021 caacagcagt tacttataga gtaattaaag gaaagtatgt taactcaaat ataatgaaaa
     4081 taaattttaa tattgtcagt ggattatttc ctattatatc tttcattgca tcatcatttg
     4141 gtatgtcaaa aagtgatatt agaagaatat atataaagtt aaataatgaa tatatataca
     4201 gtaataaata taattttaat ccagaggata taataatttt aatacctcat tgtattcaag
     4261 agaatagttg taaattaaaa gttactaatg atatagataa ttgtaaagaa tgtggaagat
     4321 gcaacatagg agaattaatt aagctaaagg aaaaaactaa tgtaaaaata tttgtagcta
     4381 ctggaggaac cctagctaga aaaattataa tggataataa acctaaagct gttgtagctg
     4441 ttgcatgtga aagagatttg acatctggaa tacaagatat taagaaaatt cctgttttag
     4501 gtgtatttaa caaaagacct aatggacctt gtgtaaatac taatgtagac attatggata
     4561 ttgaaaaagc tataggcttt ttaacaggaa aaaatatatt agtttgtaat taaactgttt
     4621 ttaagtgaag tgaatattaa gaggatgtat aataaaaata tattttagga gagtgttatt
     4681 tatgtaccct gtatatggcg gtttttgggg atttgaccca acaatggttg tattaattcc
     4741 agctatatta cttactatat atgcacaatt taaagtgagt tcaacaacaa ataagtattt
     4801 aagagtaaat acacgtcgag gatatactgg agagcaaaca gcaagaagag tacttgattc
     4861 aaatggtttg tatgatgtaa agatagaaat ggttagaggg catctaagtg accattatga
     4921 cccaagaaga aaggctgtaa ggttgtcaga agatgtatat tatggaacat ctataacttc
     4981 tgttgcagta gctgcacatg agtgtggtca tgccatacaa catgctaaag gttatgcacc
     5041 tcttcaaata agaagtagtt tagtaccagt agtgaatttt gcttcaagta tatcttggtt
     5101 tttaatattt ttaggattta ttatggctgg tccattttta aaaattggta tactattgtt
     5161 ttcggcttca gtgttattcc aaataataac attgcctgta gaatttaatg cttcaagtag
     5221 agcaatcgtt caacttggga atttagggat tatagatgaa agtgagtcaa gacaaagtag
     5281 aagagtactt tcagcagcag ctttaacata tgttgcagca gcattggttt caatacttca
     5341 attacttaga ttgttactaa tagctcaaag aagaaatgac taaaagtaaa gtgggttgtt
     5401 tagaaacaac ccttttttat gtttaataaa agggagaaat ttatatggat gcaagagaag
     5461 ttggatttaa agtattatgt gatatagaaa aaaatgacaa ttattcaaat attgcaataa
     5521 ataagcattt taaaaattta gaaataagtg atatggatag aggacttgca acagagttaa
     5581 tttatggagt aatagaaaat aaatattatt tagattatat aatcaataaa ctttcaaaaa
     5641 taaaggtaaa aaaaatgtca acttatgtta aaatcttttt aagaatggga acatatcaga
     5701 ttttattttt agatagtata tcagattatg cagcagttaa tgaaactgta aaattatcta
     5761 agaaatatga taaaaaatct tctggtttca taaatgctat tcttagaaat gaaataagag
     5821 ctaaagagac tataatggac ataactgagg aagatagtgt aaaatactta tctataaagt
     5881 attcctataa ctcttggatt ataaaaaact ggatagataa atttggacaa gaatttgctg
     5941 aagatttgtt ggaagcaaat aatgaaaagc ctagtatcta tataagaacg aatacactta
     6001 aaataagtag agaagaactt attgaaaaat taagtgaaat gggtattgta tgtttaaaag
     6061 ttcctatggt agaagaagct attaaagtcg aaaaaatgaa aaatatagaa aaaaatgaat
     6121 tatttaaggc tggtttattt acaattcaag atataagttc tatgatagta gggaaagtaa
     6181 ttaatccaaa agaaggttca tttgttttgg atgtatgtag tgctcctgga gggaaatcta
     6241 ctcacttagc tactttaatg aacaatactg gtcaagtagt agctagagat atttttgagc
     6301 ataaactaaa gcttataaac tctactgtaa atagattggg gcttaaaaat gtgtttgtag
     6361 aaagatttga tgcttctgag attgatgaaa atagtataag taaatttgat tatgttttag
     6421 cagatgttcc ttgctctggt tttggtataa taagacgtaa accagaaatt aagtataaga
     6481 aagaagaaga gttaaaagac atcacttcta tacaaaagaa aattttagaa aatgcatcta
     6541 aatatgtcaa agttggtgga actttagtat atagtacatg tacaattcaa gatgtggaga
     6601 atataaatat agttacttct tttgttgaag aaaatcacaa ctttgaattt gttccaattg
     6661 atacagtaaa tgtagactta gacaatcaag ataaaggata tttaaagata tatcctaata
     6721 ttcatggtat agatggtttt ttcatagcaa aattaaagag aataaggtag tgagagaatg
     6781 gaaaagaaaa agatagtatt aaaaaatttt actgaagatg aacttaaaga gtttatgaaa
     6841 actatagatg aaaagccctt tagaggaagt caaatattct cttggatata taagggtgca
     6901 aaaacttttg atgatatgaa caatatacca aaaagtttga gaaataaatt agaagaggtt
     6961 tcatgtatag ggcatataga tatagaatta aagttagaat ctaaggtaga taatacaaaa
     7021 aaatatttgt ttttattaga tgatggaaat ataatagaaa ctgtgatgat ggattatgat
     7081 agtagagtta ctgtctgtgt ttctaaccaa gtaggttgta ggatgggatg taatttttgt
     7141 gcatctacaa tggatggatt aattagaaac ttagagccat gggaaatttt agaccaagtt
     7201 attaaaattc aagaagatac tggtaagagg gtatcaaatc ttgtattgat ggggagtggt
     7261 gagccacttg ataactttga aaatacaaaa caatttttaa aaataattaa cgaaaaaaat
     7321 ggtcttaata tagggtatag acatataact ctttcaactt gtggaatagt acctaaaatg
     7381 tatgagttgg cagacttaga aatagctata aatttagctt tatcacttca ttcaccatat
     7441 gatgaagaac gaagaaaaat tatgcctgta gcaaatgctt attctattga agaaatattg
     7501 aatgcatgta gatattatat aaaaaagaca aatagaaggg ttacatttga atattctctt
     7561 ataaaaggag taaatgactc agaaaaagaa gcaaaggcat tagctaagct cctaaaaggt
     7621 atgctatgtc atgtaaattt aattccaata aataaagttg aagaaagaga atacgaaaaa
     7681 ccagataaag catttattta taaatttagg gatagtttgg agaagaacaa tatacctgcc
     7741 acagttagga tgtctatggg ttctgatatt agtggagctt gtgggcaatt gagaagaaaa
     7801 tacaagtaat ttgtaaggag gaagtcgtat ggtttatagt tgtgcctccc atataggaaa
     7861 aataagaaaa aacaatgaag actattgtga gggagaagtt atagatacag aacatggtcc
     7921 aataggaata tttgccatag ctgatggaat gggtggacat aaaaaaggtg aagtcgccag
     7981 taaattagca gtagaaaata taattgattt tctaaaggaa aacttattac aacatgataa
     8041 tgtaaaaata gactatatag atgatatatt aaaacaggct tataataatg taaactcaat
     8101 tgtgcacaaa aaatctatgg aagatataga atttgaagga atgggaacta cattagttac
     8161 ggctatagtg tataataatg tcttatatgt ggcaaatgtt ggagatagca gatgttattt
     8221 gttaacagat gaaaaatttg ataaaataac tatagaccat tccgtggttg aagaacttat
     8281 gatggcccat gtcattacag aagaagaggc aagaagacat ccacagagga atagaataac
     8341 tagagcaatt ggaacagatg atatggttgt tgtagatatt tttaagaaag aaatcaaaaa
     8401 aagtgatata atacttcttg cgacagatgg attaactggg tttattgatg atgaagatat
     8461 aagagattta atattagatt atgaatatga gagaactagt aatataagtg aagaattgat
     8521 aagtatggcc aatgatgttt ctggtaaaga taatgtttcg gttatagtga taaaagtata
     8581 attaggacgg tgatattgtg ggagatacaa ttttaggaaa tcgatatgaa atcatcagga
     8641 agattggtga tggaggaatg gcctttgtat atgaggctaa agacagatta ttaaacagga
     8701 ctgttgcact taaagtactt agacctgaat ttgtagatga tgatgaattt ctaacaaagt
     8761 ttaaaagaga ggcagaagca gtagcaagtc tttcacatcc aaatatagta aatgtttatg
     8821 atgttggtga agatggaaaa gttcactaca ttgtaatgga gtttgtagat gggaagaatc
     8881 taaaagagat tatacaagat gaaggtatat tagatgaata tactgcatta gatataacaa
     8941 aacaaatagc tatggcactt agtgcagcgc acaaaaaggg gattatacat agagatataa
     9001 aacctcataa tattctcata tcaaatgaag gaagagtagt aaaagtggct gattttggta
     9061 tagccaaagc tgtttcaaat tcaacaatga ctaatattgg tagtataata ggttcagtgc
     9121 attatttttc accagagcaa gctaaaggta aatttgtaac taataatgct gacctgtatt
     9181 ctctaggtat agttttatat gaaatgttga taggaaaagt tccatttagg ggagatagtc
     9241 caatttctat agcacttcaa cacattaatg atgatataga ctttacatca gaagaaaaag
     9301 ttaggattcc tcaaagtgta agaactacaa taaaaaagct tacagaaaaa tctagtgcag
     9361 atagatatca aactgctgaa gaattgatag aagatataga gtatatagaa aaaaatattg
     9421 atttagattt tataaaagaa tatgatgatt ttgcaactaa gaaaattgat gaaaaagaaa
     9481 ttaataaagt tgtaaatcca acacttgcaa aaccagctcc tgaaaaagtg gttaagcctg
     9541 ttgaagtagc tgatttggat gatgatgaag attactatga tgatttttat gaagaagatg
     9601 atgatgaaga tgaagaagag gaagagataa tgagagctaa aaagaatcag agacctaaga
     9661 gtactccaag taagagaact aagaaaaaga agaaaaaaca ggaaagccca aaatctagaa
     9721 gaagattaaa agtaattgcg gctgttttga tactgatatt gtgtgctcaa gtattcttag
     9781 catataagtt tttatttgct ggagggtttg gcaataaatc tttgactgtt cctaatcttg
     9841 taaatatgac attagaagaa gctcaaagtg ctgttgaaaa agaaggatta tacctgagtg
     9901 taaaaagtga agagtataat agtgaagttg atgaaaattg tataatatca cagacacctg
     9961 aaggtggaag tacaaatgtc aaaaagggtg atactataaa tgttgtcgta agtaaaggtt
    10021 caagtcaggc atctgttcct aatgtagtgg gacttacttt gagtaatgca aaacaactta
    10081 ttgaagaaaa taacttaaaa gtgggaactg ttaagtatga atacagctca atatataaag
    10141 aaggtacagt tttaagtcag agtccgggtg ctggctcatc aagagctcag gaaggtgatg
    10201 aggtaaacct atatgtaagc aaaggttcag aaaaatcaaa tacacagact ccaacagtac
    10261 cagataaaaa accaacaaca ccagagaata atacaccaac agaacctggt tcaaactcag
    10321 gaaacagtgg tggaaattca ggaggctcaa attcaggaaa tagtggtgga aactcaggca
    10381 ataatggaag taactcagga ggttcaaact caggaaatag tggtggaaac tcaggaggct
    10441 caaactcagg agataatgga ggaagttcag gaggctcaaa ctcaggagac aatggaggaa
    10501 gttcaggagg ttcaaactca ggagacaatg gagatagttc aggaggctca aactcaggag
    10561 ataatggagg aacttcatca ggaactggcg caaacattgg taaaacagaa taaatattaa
    10621 taaaataaga gctgtccttt tatagaaaat attttcttta aagggcagtt tgttttgtat
    10681 aaaatactga attggttata atataaggag aaaagtattt atgaagaaga aaacagtttt
    10741 aattacagga ggagctagag ggattggtaa agctatgtct aaagcttttg caaaagaagg
    10801 ttacaatgta ttagtaaatt ttaataagtc agaaaatgaa gctaaagagt tgtacactat
    10861 attaaatgaa aaaaattttt ctgtaaaact ttttaaggct gatatttcaa atagagaaga
    10921 tgtagaggat atggtagatt attgtataaa agaatttgga ggattggatg tacttgtaaa
    10981 caatgctggt gtaagtcaag ataagttgtt tacagatata actgatgaag attgggataa
    11041 tatgatgaat attaatttaa aaggaagctt ttattgttca caagttgctt taaaatacat
    11101 gatatcagaa aaaaaaggaa atataattaa tatatcttca atctggggta tatcaggtgc
    11161 gtcttgtgaa gtgcattatt ctataacaaa agcaggcata ataggtatga caaaggcatt
    11221 agctaaagaa gtaggaccat caaatattag agtaaatagc atagctccag gtgtcattaa
    11281 tactgacatg ttatctggat ataatgagga agatatagac gctcttgtgg aagaaacccc
    11341 tctaatgagg ctaggaactc cagaagatat agcaaattgt gctattttct tggcatcaga
    11401 caaatcaaat tttatcactg gtcaggtaat tagtccaaac ggaggatttg ttatttaaaa
    11461 aaaatgtggt attatataaa aataggaaag aataacacga tatactactc ataaacagta
    11521 tgtgtttata taaattttat aaagggaggc atttgatgct agaaggaaaa attataaagg
    11581 ggattagtgg attttattat gtagatactt ataatggtat ttatgaatgt aaggctagag
    11641 ggatatttag aaagcagaaa ataacacctt tggttggtga tagagtcaaa ataagcattg
    11701 ttgatgaaaa tgaaaaaaag ggtatcttag aagaaatcga cagtagagat acagaactta
    11761 taagacctcc tattgcaaat gtagataaag cattaatagt attcgcaata aaaaatccta
    11821 aaccaaactt atctctttta gatagattta tagttttggc agaaaaagaa aatttagaaa
    11881 ctgtaataat ccttacaaaa gcagatttag atgataatga tacattagaa actgttaaaa
    11941 atatatatga acttagtggc tataaagtca ttcctgtaag caatattact aagttaaata
    12001 tagataaagt aaaagaagaa ttaaaagaaa atgtagtagt gtttgcagga ccatctggag
    12061 ttggaaaatc aagcttatta aatgaaatag atgaaaattt taaacttcaa acaggagttg
    12121 taagtgataa aataaaaagg ggaaaacata ctactcgtca cgctgaactt ttaaaattag
    12181 aatttggtgg aatggttgca gatacaccag gttttagttc ccttgccctt gaagatatag
    12241 aggaagttga acttaaagat tattttatag aatttgataa atttaatgat tgtaagtttg
    12301 gttcaaagtg tattcatgaa aatgaaccta attgtgctat aaaagaagct gttacaaatg
    12361 gagaaatatc taaagaaaga tatgatagtt atatacagct gctacatgaa ataagacaaa
    12421 ataatagtag gaggtattag aaacatgatt aaattagcac catcaatatt atcagcagat
    12481 tttgcaaaat tattagaaga tgtaagaaaa gtagaaagtg caggatgtga atatcttcat
    12541 atcgatgtaa tggatggaca ctttgtacca aacattacat taggaccact tgtagtcaaa
    12601 agtttaaaga aagaaaatat aaatatggta tttgatgctc atcttatgat agaaaatcca
    12661 gaccagtata ttgaagaatt tgtaaaagca ggatgtgata taataactgt tcaccaggag
    12721 gcttgtgttc atctacatag aactatacaa aatataaaat cacatggaat aaaagctggt
    12781 gtagtactta atccagctac accagtagat acgattaaac atgtactacc tgatttagat
    12841 atggtattat taatgtctgt taaccctgga tttggaggac aatcatttat tccttgtgtt
    12901 ttagataaga ttaaagagtt aaaagctatt atagatagtc aaggattaaa cattgatata
    12961 gaagttgatg gtggtataag ccctaaaaac gtagccgaag ttgtacaagc aggagctaat
    13021 gtaatagtag caggttctgc aatatttgga agtgatgaca ttcaagaaac agttaattta
    13081 tttagaaaga atgcttctct tgaagaatta gtttaaattt aataatgaaa atctgtattg
    13141 ttttaaatgg cgaaattgaa gattacgaag ttacaagaga tattataatt aaggaatgtt
    13201 atgactgcat aatatgtgca gatggagggg caaatcacac ttataaaatg gaaataatgc
    13261 cagattatat attaggagat ttagattcag tagaagaaga aaagataaac ttctataaaa
    13321 ataaaggtgt taaatttgaa aaatttccat ctaaaaaaga tgagactgat acagagcttt
    13381 gtcttttttt agctaagaca cttaaagcaa atcatattga tttttttggt gctttagggg
    13441 gaaggattga ccacactctt gcaaatataa agctattata ttatttaaaa gaagatggta
    13501 tatattctag gatactttct gataaagaag agatgtatat agttgaaaat gaggaaattt
    13561 cattgtatgg aaacccaggg gacactattt ctgtaatagc tataaatggt gatgctaagg
    13621 gtgtaacact tactggctta gaataccctc tcgataatta ttatatgaaa tactctgtac
    13681 ctattggaat atcaaatgtt atgttgagta attcttgtaa gattaaagta gagcaaggct
    13741 gtgtacttgt agttagaaat ttataaaatt tgattttgac aattggtttt atattgtaga
    13801 gcagatacaa tcaagtaaaa tatatttatg attgtatctg ctttttagta tataattata
    13861 aatatataat aaactttgac ataaggggga ttttttgtgg tagatagtat tatttttgat
    13921 ttagatggaa cattgtggga ttcaactgaa gaagtatgca aagtctggca ggatgtttta
    13981 agtgagcata aggaaataga attaagtgtc acaaaagatt tatttagaag tcttatgggg
    14041 ctttcttttg aggaaatagc aaatcgttta tttccaaatt taagtgaaga agaaagaatg
    14101 aatttattga atgaatgttc tttgaaagaa tgtgactatt tatctgaaaa tggagctaaa
    14161 ttatttgatg atatagaaga tactcttaaa ttactatcta aaaaatataa attatttata
    14221 gtaagtaatt gtcaagttgg atatatagaa tcttttttaa aagcacataa actagaacag
    14281 tattttatag attttgaatg tcctggtaat actggtttgc ataaaggaga aaataataaa
    14341 cttataattg aaagaaataa attaataaat ccaatctatg ttggtgatac gcaaggtgat
    14401 gcaaatagtg ctaagtttgt aggtatacct tttatatatg ccaaatatgg gtttgggaat
    14461 gtagatgtat atgattattt tatagattca tttaaagatt tgttagaaca tgatatatta
    14521 aaataagatg agtttaaaaa ttatattaaa ataataaaaa agttatgggg ggatttaaaa
    14581 atggaattac aagatactat ttttaaaaga caaagtgtaa gaaagtttaa aaatcaagat
    14641 gtatcagatg aagatatttt aaaaatgata aaagcagcag gtgcagcacc atcaggcaag
    14701 aatattcaaa actggcattt tgttgttata aaacgccgtg atttaatgga aaagatagca
    14761 gatgtgataa ctaaaaagca acaagaaata cttgtagaaa tggataaagt atcagtggat
    14821 aaggctaata gatttagaaa atttgttaaa aattttacac tattttactt aaaagctcca
    14881 gttttagtat tagtttttac aaaggtatat aatccatcag gatattatga attagagctt
    14941 atagatgcac ctaaggagac tatagataaa ttatttataa gaaatccagg tatgcagagt
    15001 ctaggtgctg caattgaaaa ctttacacta tcagcaattg aacttggata tggttcttgt
    15061 tggttgacaa gtcaaaacta tgcagcagat gagatagaag ctgttttaga agcagaaact
    15121 ggttttgaaa agggagagta ttttttaggt gcaatgttag ctcttggagt gccagaggac
    15181 aatttgaaga gtccatctaa aaaaccagta gaagaaatat gtacatttat aaaataaaag
    15241 tcaaggacat tttaaaatcg aagtaaatta aaaacaaaat aaattaaaaa taaatattta
    15301 aaaaattaaa aagtaggttt tattgatatt aattttatat ttcaaatcat tgatttaatt
    15361 aatacaataa gaactacttt ttttgtatat taattttaaa tatatttaaa tctttagaaa
    15421 atctttagaa aagtatatgt aatatcttaa gtttttaatt ttaatattaa gtatataaaa
    15481 caaaatgaaa aaatattaaa tttaaactag aaggaggttt ctaagatgga cattaaatac
    15541 aaataaccag aacaa
//
LOCUS       DAGSHT010000071        15508 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGSHT010000071 DAGSHT010000000
VERSION     DAGSHT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.073"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(29..505)
                     /locus_tag="MJB72_001991"
     CDS             complement(29..505)
                     /locus_tag="MJB72_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBY8263099.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(508..912)
                     /locus_tag="MJB72_001992"
     CDS             complement(508..912)
                     /locus_tag="MJB72_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBY8263100.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(887..1516)
                     /locus_tag="MJB72_001993"
     CDS             complement(887..1516)
                     /locus_tag="MJB72_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263101.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      1882..1925
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2026..2994
                     /locus_tag="MJB72_001994"
     CDS             2026..2994
                     /locus_tag="MJB72_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBY8263102.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            2996..3559
                     /locus_tag="MJB72_001995"
     CDS             2996..3559
                     /locus_tag="MJB72_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY8263103.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            3567..4256
                     /locus_tag="MJB72_001996"
     CDS             3567..4256
                     /locus_tag="MJB72_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8263104.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            4383..5111
                     /locus_tag="MJB72_001997"
     CDS             4383..5111
                     /locus_tag="MJB72_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY8263105.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            5125..5799
                     /locus_tag="MJB72_001998"
     CDS             5125..5799
                     /locus_tag="MJB72_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY8263106.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            5825..6661
                     /locus_tag="MJB72_001999"
     CDS             5825..6661
                     /locus_tag="MJB72_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263107.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            6677..7348
                     /locus_tag="MJB72_002000"
     CDS             6677..7348
                     /locus_tag="MJB72_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263108.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            7338..8570
                     /locus_tag="MJB72_002001"
     CDS             7338..8570
                     /locus_tag="MJB72_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263109.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            8720..9037
                     /gene="trxA"
                     /locus_tag="MJB72_002002"
     CDS             8720..9037
                     /gene="trxA"
                     /locus_tag="MJB72_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBY8263110.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            9054..9932
                     /locus_tag="MJB72_002003"
     CDS             9054..9932
                     /locus_tag="MJB72_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY8263111.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            10202..10570
                     /locus_tag="MJB72_002004"
     CDS             10202..10570
                     /locus_tag="MJB72_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY8263112.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            10573..10944
                     /locus_tag="MJB72_002005"
     CDS             10573..10944
                     /locus_tag="MJB72_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBY8263113.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(11089..11544)
                     /locus_tag="MJB72_002006"
     CDS             complement(11089..11544)
                     /locus_tag="MJB72_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263114.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(11583..12848)
                     /locus_tag="MJB72_002007"
     CDS             complement(11583..12848)
                     /locus_tag="MJB72_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBY8263115.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            13318..14769
                     /locus_tag="MJB72_002008"
     CDS             13318..14769
                     /locus_tag="MJB72_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBY8263116.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(14988..15449)
                     /locus_tag="MJB72_002009"
     CDS             complement(14988..15449)
                     /locus_tag="MJB72_002009"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBY8263117.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT         5983 a         1700 c         2482 g         5343 t
ORIGIN      
        1 atttatcata gaaataatta gctttatttt agctattaag atttattaaa aaattatcta
       61 tattatcctg tgatattcta aaactgtcat aattaccttt tgaaacagcc ttcataataa
      121 gtttatctaa aaatttcatt ttttctaatc gagcttcact accaaaaatt ccaatagcat
      181 ttgcactttc taataaggat gatggaaaat ttgattttaa atatttatct gcttgttcag
      241 aaaagccaca gcaaaggaaa atgccaactt gttttttgtt taataactct acattatcat
      301 tacatagcac ctgtattttt tttgaaactg aaccaacata tatagaactt ccaataataa
      361 ttttatcaaa ttttgataac tcaattttat tattattatt attaatatca acaatagtga
      421 cattatctga tagtttattt tttagtatat ttgcacagtc ttttgtacaa ccatatttac
      481 ttgaatatat aataatagta ttcataatta ttctcccttt cttaaagcat tttctacagc
      541 ttttcgtata gtattactgc tataagttgc agaagttata gcatccacat ctaaagactg
      601 ttgtttaata atacttgttg taattttttc agcaggttta cctaaaagat ggtcgtgttt
      661 taagatatta atattttgga ttttaccatt attaacttca acagacacag tagcttttac
      721 caagttatta tcagcacttc cagtataaat gccattttta acattattaa tatctatatc
      781 ttttactacc aagttttctg gctttaagtt gcttacaatt acaataataa tgcatataaa
      841 tacaaagaat agagcaatag atatttttac atattttttc tttttattag tagccttgtt
      901 gcgaattttc attgtaaagt cctcccacta aaatatctaa gaaattttct ataagttgag
      961 aaaaattatg taaagaatat agtttatttt caacaacaca taaaacgaat cctaacaatg
     1021 cacttataag catccatgag cttttatcta tatttttttt gaatattcct tgttcaattc
     1081 caatggaaag gaatttatca agcattttta taccattatt gctaggcgta ttgtttgtat
     1141 tttcagatgc aaatataata ttcattctac tatgcatgat agattttgcc atctctggtt
     1201 cactagaaag agtttttata aagctagtca taatttctat gatttgttta tctattggta
     1261 gtgatgagca attattcata atgtcaatgg cattactttc tactgtgtta taaagtttat
     1321 tggtcatatc gctgataatc tctgctttgt ctttgtagta aagataaatt gtagttggag
     1381 aatattctat ttttgtagca atttttctta ttgataaatt ttcatatcca tgttggttta
     1441 taatttcgat gctagcatcc ataatctttt tcttcatttc ttctttttca tttttacgtc
     1501 tttctttgat actcatataa tcacctcttt gcttaacggt gttaagttaa tttacactgt
     1561 tattgtataa tttaatttta atactgtcaa taaattatta aataatataa gttaatatat
     1621 ttttatggat aataaattaa tgttattaat tgtaatattt aagatattat tgtataataa
     1681 taatcggttt aaatgcgcca catcttatta aagttggttc ataattctaa ataaacttta
     1741 ataagataca ttaaaatata ataaattctt ttatgctata aaatttattt tatagctatt
     1801 aaatcagatg tttaaaaatt ttttaacggt ctatagtttt taagagtctt atagtaagta
     1861 gcctaaaaat atgattttac atctgtactt gttcgagaac tccaagtata aaaaactaag
     1921 gaattatata gtggcagtat ataattaata cccttagaca cacctttttg aaagtggaaa
     1981 aaagatgtgt agttctcctc gaccacaaat caaggaggat aaaaaatgga aaagagaaaa
     2041 gtaataattg attgtgaccc aggaattgat gattctttgg caattcttct ggctttaaac
     2101 tcaccagagc tagaagtaat tggaattacc acatgttgtg gaaatgtccc agcaaatata
     2161 ggtgcagaaa atgcactaaa aacacttcaa atgtgttctt cactaaatat tccagtatat
     2221 ataggagaag aagcaccact aaaaagaaaa cttgtaacag ctcaagatac acatggagaa
     2281 gatggtattg gagaaaactt ttatcaaaag gttgtaggag ctaaagcaaa aaatggagca
     2341 gtggatttta taataaatac tttatataat catgaaaaag tatcaataat agcacttgca
     2401 ccacttacaa atatagctaa agcacttatt aaagataaga aagcatttga aaatctcgat
     2461 gagtttgtat ctatgggagg agcatttagg attcatggaa attgctctcc agtagcagag
     2521 tttaattatt gggtagaccc acatggagca gattatgttt acaagaattt atctaaaaaa
     2581 atccacatgg taggtttaga tgtaactaga aaaattgtac ttactcctaa tattattgag
     2641 tttataaata gacttgataa gaagatggca aagtatataa ctgaaataac tagattttat
     2701 attgatttcc attgggaaca ggagggaata attggctgtg tgataaatga ccctctagca
     2761 gtagcatact ttatagatag aagtatatgt aaaggatttg aatcatatgt agaagttgta
     2821 gaagatggaa tagctatggg tcagtctata gtggattctt tcaatttcta taaaaaaaat
     2881 cctaatgcaa ttgttctaaa tgaagttgat gagaagaaat ttatgtacat gtttttaaag
     2941 aggcttttta aaggttatga agacattata gactctgtgg aaggagtgat atagtgtgaa
     3001 aagaaaaata tgtgtaaaaa ctattacact tatagctttt ggtatttcta taaatataat
     3061 aggcgctttt atagctatgg gattaagatt acctgtatac ttagattcaa taggaactat
     3121 aatgatagct tcacttttag gacctaaata cgctgttgtt acaggtgtgt ttggaagttt
     3181 aataagtgga attacgtttg atgtgtattc tctctatttt gcaccagttc aaatatcaac
     3241 cggattactt gctggaatag catttaaaaa gaatttttta aggggtttaa aaactccact
     3301 aggagtactt ttatttgcca ttccaacatc tataataagt tcaataattt cagcattttt
     3361 atttggaggt atgacctctt caggttcatc atatatagtg caaatcttga aagtgttagg
     3421 actgggggat gtttttagtg tgtttgtaac ccaagtattt actgattatg gagataagct
     3481 gttagcagtt gttttagtga atttaggatt aaatgcagtt ccaaaaactt taaaggtatc
     3541 attgactgga ggaaaataga tagaaaatgg atagatatag tgagataaca aataaaaatc
     3601 aaagagaaat tgttctgctt aaaggatttc cttgtatatg gggaaagtgt tctttttgtg
     3661 attatataga tgataattct aatttagaag aagagatgaa taaattaaat ctaaaggttt
     3721 taaaaaacgt aactgggaaa tatggagttc ttgaagtcat aaattctgga agctgttttg
     3781 aattacctaa ggatacttta gaaaaaataa agtgtattat aaaagagaag aatataaaaa
     3841 aattattttt agaaagtcat tggtcttaca aaaataggct taaggaaatg agagagtatt
     3901 ttgaaattcc tgtggtattt aaaattggag ttgaaacctt tgacaatgat tttagaaata
     3961 atattttaaa taaaaatgca aattttaaga ctccacaaga tgtaaaagaa tattttgatt
     4021 caccttgtat tatggttgga ataaaaggac aaacaaaaga gatgatagat aaagatatag
     4081 agattatcct aaatactttt gaaaaagcga cagtgaatgt ttttataaat aattcttcta
     4141 gtataaaaag agatgaagag ttagtgaaat ggtttgttag taagtataaa ttcttggatg
     4201 aaaatccaaa tatagaagtt ttatacaata atacagactt tggtgtagga gattagcatt
     4261 aatttagttt aaatttaaaa ttgaaaagtt tcttatgttt ttgaaaaagc ttctaatagt
     4321 tagaagcttt ttttgtagag ttttatgtta caataagaaa aaaggaatta aggagcaatt
     4381 aaatgatgtt tgatagggga aaatggaata aattggatta taatgaactt ttagaatata
     4441 tgatgtctat acaagatata aaatatagag attttaataa aaagcttata cctggaacag
     4501 aaaatataat aggtattaga gttcctaatt taagaaagtt atcaaaagaa ataagccaag
     4561 ggaattggaa ggaattttta gaagtagcac aggatacata ctatgaagaa gttaggctac
     4621 aaggtatggt aattggcaat ataaatagta attttgaaga aactttatac tatgtaaaaa
     4681 gatttatacc taaaatcgat aattggtcag tttgcgatgg attttgttct gatttaaagt
     4741 ctatgaaaaa atataaagaa aatatgtatg atatacttaa aaaatatgtt tattcaaaga
     4801 atccatggga aattagattt gcattagtta tgtttttaat atattatgtt gatgataaac
     4861 atattaaaga aatatttgaa tactgtaata atatacaaag tgaagaatat tatgttaaaa
     4921 tgggtatggc atggttgcta tcaatttgtt ttattaaatg tgaagaggaa acttttctct
     4981 atataaaaaa taataattta gatgatttca cttataataa aatgctacaa aaaataattg
     5041 aatcaaatag agtagattta gaaaagaaga atataataag aagtatgaaa agaaagacaa
     5101 gaagatgttg acggaggaat attaatgaat aacaatgaag acagaattga caaattttta
     5161 gaacttcaaa gcaaaggatt taaatttgca gagatagctc aaaatctaaa gattagtcaa
     5221 agtactttaa gaactttttt aaataaaaaa ggatataagt cagaaaaagg taaatacata
     5281 ttaaaagacg aattaaatta taataaaagt ttggataata gaaacttgga agaagagaaa
     5341 gctaaggagt atgaaaaaag taaaaagaat actataagta atagaaaaaa agtaagcacc
     5401 aaaaaaaaga tttctaatga aaaggcttta agtaaaagtg ttgaacaaat tgagtttaat
     5461 gatacaggtt taaaaaatgc aaagccaaaa caaaatatta agcctaagaa agatagaaaa
     5521 ataaatataa ctcaagaaga tttagataag ctttgtgaag tatatgattg gtatttagaa
     5581 gtaaaagatt acaatataaa gcctaaaaaa gtatctaata aaaaagatgt acatatagat
     5641 gatgaacaca tagatgaatt aaaaacaaca agaatacgta tagataaaga aatatggaat
     5701 gattttgaaa gattatgtag taattcgaat tttgaaaaac atcaaatact tacacaggca
     5761 ttgaaagaat ttatgaaaaa gtataaaaat ctactataaa atactactat atgaggtgct
     5821 taatatgaat aaaatagtaa aaaaatcaat ttctctaatg attatattaa caatatttat
     5881 ttttatgttg acagcatgtg aaaaagatga acagcctgat tcagttgata ttcaaacaga
     5941 agataaaaat gagataaaaa tagatgaggg aaatgcaaaa gtattgaata taggaaaatc
     6001 agaaattata aatatagatg aaggtgataa agtagataca tcaacaaagg tagaaaataa
     6061 ttcaacattt aatatatcca atgttgaatt gatatataat gaatatgatg agaataaaaa
     6121 aattatatct tcggatagta aggctttgtt ggacatgact ttaatgcctg ggaaagttgc
     6181 ttacatagaa tgtgggcata aaacttttgt aaagggtgta gaagtttatg catatgaata
     6241 tgaggcagag ggtaagattg tgtatgttaa tttaaaagaa aatactatta atataagaaa
     6301 taataatatt aagctagaaa atagtagtca gtatgaggtg ctatctacat cagaacttaa
     6361 aaaagtaaat gaatcaaatg agggaattac gtatcaagta aaagtaaaaa attcatcatc
     6421 aaaagattta ggaaatatta ttcttaagac agcagaagtt aatgaaaatg gagaatattt
     6481 aacagtaaat cgtgtaccat catataagat tttaaagcct tcagaagaga cagatataga
     6541 tatattgtgt tcaacaaagg caaaaagtgt tgaaatagtg ggatatacgt atgatgatat
     6601 aaaagaaaaa gccaatgtgg atattgactt aaaatctcat aaagttaaga tagacaaata
     6661 aaggtagggg atacttatga aggttttaat agttgaagat aataagatac ttttagaaag
     6721 tgtagtagaa gaacttagca agcattttga aactgaaaag tgtgaagatg gagaagaagc
     6781 tttatactta ataaatcaaa atatatatga tttagtcatt ttagacttaa tgcttcctaa
     6841 tataaatgga tttgatatcc taaagaaaat gagaattaat aatatagata cacctgtact
     6901 tatattgaca gcaaaagaaa cattagatga caaagtagaa gcatttacta taggagctaa
     6961 tgattatttg acaaaacctt tttatatgga agagttagta gctagggtat acgctatatt
     7021 aaggactaat ggaaagataa aagaaagaaa cggattagaa tttaaatctt tatatttaga
     7081 tacactagaa aaaagagtgt atattgaaaa agaagaaata aaacttcaaa acaaacagtt
     7141 taacttgctt gagtattttg tattaaataa aggttcaatt ttattaaaag agcagattta
     7201 tgatagaata tggggaattg attcagatgc aactattgaa atagtggaag tatatgtaag
     7261 taatctaaga aaaaagctaa gtaaatatgg atatgataaa tatattaaaa ctaaacgtaa
     7321 ggtaggatat atattcgatg ataaataaga atgtatttac cagtactaaa aatcatttaa
     7381 taaaaatgta tattattgta gtaggtagtt ttttaataat cttttctata tttatatact
     7441 catattttag gggacttaca tatagtggaa ttgatagtga gataaatgat gaacttgaat
     7501 atatagtctc tcaatttaaa agaacttcat ttttgaaccc aataagactc aaagatccta
     7561 aagatatggt gtatgtatat gaagatggaa ggataagtta ctatactcaa aatgaatatt
     7621 ttgatgagtt acttccagat agaagactag ataaaaaaaa ttcgtttttt aaatacacag
     7681 aaaatggata tacttttaga gaattaaatg tagatgtagg tagataccaa atacagataa
     7741 ttagaaatat agattctgag atgaattctt taagacagct tacatctgtt cttataattg
     7801 gaatattaat ttctgtaata attacttact ttgtggctgt atatctcact agaaaagctt
     7861 taattccaat agaaacagca tggaaaaatc aagctaagtt tatacaagat gcttctcatg
     7921 aattaagaac accaataaca atagtttcat caaaattaga aagtatgtta aaatccccag
     7981 aaagtacagt aaatgatgaa gttgaaacaa tagctactgc aatgaaggaa actagaagac
     8041 ttaaaaaaat gataactgat ttattgtctc ttactaaaga ggactctatt gttaaagtta
     8101 atttagaaga aatagattta gaaaagttat tggaagaaat atcagaagac tatattgaca
     8161 ttgctgagtt tcaggaaaaa agattcgtgt ttaattctaa actgaagaat aaagttataa
     8221 taacagataa aaataaatta agacaactta tattaatttt cattgataat gcctttaagt
     8281 atacaaaatt gggtgatgag atagcattag aattgaaaga agatatagag gatgaggtaa
     8341 ctttacttat aagtgatact ggtataggca taaaaaaaga agagatacct cttatatttg
     8401 atagattttt tagaagtgaa aatgtaagaa ataaagattt agaaggttct ggaataggac
     8461 tttcaatagc tcgcatgatt agtttaaact tatctattga tataaatgta acttctgatg
     8521 tagatattgg aactacattt gaattgtcaa taccaaaaaa gttaaaataa taaaaatata
     8581 aaaaattgtt tttagaaatg aagaatgggt atactaatta gtataaaata taaaaagacc
     8641 ttataaaagt aaatttatat ttataaattt catagtataa ttatataggg ataataaagt
     8701 ttaataggag gatttaaaaa tggcaaaagt tataaataca tctgaattta gaagtagtgt
     8761 tgaaggtagt aagggagtag ttgtagttga tttctttgca acttggtgtg gaccttgtaa
     8821 catgttggga cctgtatttg cagaattagg tgaagagatg aaggataaag ctagatttgt
     8881 aaaagttgat atagatgaga gtttagaaat agctcaacaa ttcaatgtat ctacagtacc
     8941 aactatgatt atatttaaag atggaaaacc agtagaaact ttaattggat tcatgccaaa
     9001 gaacaaaatt gaaatgcaag taaaatcata tttataaaaa atgggtgagt attatgagat
     9061 atgatatagc tataatcggc agtggtccag ctggattgtc tgctgctata aatgcaaaaa
     9121 taagaaataa aacaattatc atgtttggta atgataattt aagtaataag cttgttaaag
     9181 ctccttcaat agataattat ttaggttttt atgatataag tggtgatgaa ttaaaagaca
     9241 agtttaaatc acatatagat agtatggata tttcaattga aaataaaaga ataaataata
     9301 tttatgctat gggagaatac tttacaataa tgtctggaaa tgatatgtat gaagctacta
     9361 ctgtaatcct tgcaactggg gtagagtata ctagaccaat caagggtgaa gaagaatttc
     9421 taggaagagg tgtgggctac tgtgctacat gtgatgcacc actttataga aataaaaagg
     9481 tagcagttat aggatataat gaagaatcta aagaagaagc aaatttttta agtgaactca
     9541 cttcaaagac ttattttata cctatgtaca aaaaagataa tcttatgaga agttcagata
     9601 acctagatga ttctattgaa gttatacatg acagaccagt acagatagat ggagataaac
     9661 ttgtaaacaa agtatctttt aaagaaaacc acattgaagt tgatggagta ttcgttataa
     9721 aagatagtac ggctccaagt gcattggttc ctggaataga gattgatggc attcatatta
     9781 aagttgacaa taatatgaag actagtatag atggatgctt tgctgctgga gattgtgttg
     9841 gaaaacccta ttcttatata aaagcagctg gtcaagggca aatagcagct ttaaatgctg
     9901 tttactattt agataaatta aagagggctt aatatttata ttgattaact ttttaaagat
     9961 atttataaaa aactgtcttg aaataatttt attatttcaa gacagttttt tatttttaag
    10021 ttattaataa aagtatttta aaatctatat gtaattactt ttattaataa aactcaaagt
    10081 tatattagta aaaattggaa ttatttataa tatactccaa aatttaaaaa taatggttga
    10141 aaaatctaat taagagtact ataatttaat taagtaatta cttaatcaaa atgaggtact
    10201 tatggaaaac ttggttaaaa tatttaaagc attgagtgat gaaactagac ttaacatact
    10261 tatattagta tccaagagaa atatatgtca aaaggggatt tctaaatatc tagggatttc
    10321 agattctgct gtttcacaac atataaaaat attaaaagat gttgggataa taacaggata
    10381 taaagaagga tactatgttt tatatcatat taataaagaa tcatttaata tatgtgtaga
    10441 ttttataaat tctatgttaa gtaattctag tgaaagcttt atagatgtat ttgatgtaaa
    10501 tacaatacat ctaggatgca gtaaagaatg taaatctata aaaaaatgtt gtaagagaag
    10561 ggagaaataa taatgaaagt atgtatacca gtagaggaga ataaaggatt ggatagtaaa
    10621 ccatatggac attttggttc tgctccaatt tttgttgtgt gtgatttaga aagtggagaa
    10681 gttaaatctt tagataatgg agatttagat catgaacatg gaaaatgtca accattaaaa
    10741 gcattgtcag ggacagctgt agatgcagta gttgttggag gaataggaca aggagctata
    10801 attaaattaa atggaatggg aataaaagtt tacagagcag aaggtgatac tataagtgct
    10861 aatttagact tacttaaaaa tggaaaatta gtagaatttc catcagacca tacttgctca
    10921 catgacggat gtggacatca ttaaaaaaaa acagctgatg aaatatatac ttcatcagct
    10981 attttttctt ttaataaata cgattttatt cttttaaaac caaaatttag atttttatgt
    11041 tttttgtagt atccttaata atatttcaag tttacaagtt tactactttt agattttttt
    11101 tgtatgtttt tctatatagt caatatcatt taatatgtca ttgagttctt tattaagtgt
    11161 ttttaaatca tcaccatcta cattgtagtt taaaaatagt tcctctacat tatcaatgat
    11221 agaattgact tttctagtaa cttcaacaaa tgtcttaaag ttactactat ttttagcatt
    11281 tagaaatttt tttaaatcct ttgatataaa tgatactatt tcatattcat cattaaatag
    11341 acctctacca ataggatatg gttcaattct ctgtaaaaaa tattggtctt tagcattaat
    11401 attataagta taagatattt cccaaccgtc tcctaaatca aacttgataa aatataatgt
    11461 actacttact tttgatattc ttgtggcacc aagctgtctt aaaagaactt caatcgaatc
    11521 accttgaaca gtattaatat tcatatatac acatcccctt tagtattatt atactatatt
    11581 ttttaaaatt ttataattaa ataaacagta gatataataa tagatataat cataagtgga
    11641 aaacctattt tgaaatactc cttaaaagag ataggatgac cgtgtttatt acctatacct
    11701 gctagtacaa cattagcaga tgctcctatt agtgttccat ttcctccaag acaagctcca
    11761 agagatgttg cccaccatag tggcattaca tcaatacctt cagcttgcat agtcaaaata
    11821 agaggaatta gtgttgcaac aaatggaata ttatctaaga aagaagagac tatagctgac
    11881 aaccataata tcaacaacat tgtaaataca agatgtcctt ctgtaagtcc aatcaatgct
    11941 tgagccaatt tgtttattat cccaacttct actaatccac caactactac aaataatccc
    12001 ataaagaata aaattgtaga ccattcaata cctgccataa tttcatctac atcttgtttt
    12061 cctattaaaa gcattataga tgctcctgtc agtgctacaa cagaagaatc tatgtggatt
    12121 gtactgtgaa acataaaacc aaaaagtatg aatgctatta atataaggct ttttataagt
    12181 agaggtttat ctttaacaga ttttttttca tctagtttta atattgcatt tttagctctt
    12241 tcattcacaa caagttcttt tccataaata aatctaaagc atattatagt tatagcaagt
    12301 attactataa cagcaggacc taaatttatt acaaaatcca taaaactaag attggcagca
    12361 ctaccaatca taatatttgg agggtctcct ataagagtag ctgttcctcc tatatttgaa
    12421 gctaaaatct gtgttattaa aaatggaact ggattaagtc ctaatatttg tgtaataact
    12481 atagtcattg gtccaataag taagacggtt gtaacattat ctaatacagc tgataaaata
    12541 gctgttataa tagcaaaaca aatcattatc ttccatgggt cacctttggc tttctttgca
    12601 gtccagatag ctatatattc aaataacccc gagtttttga caacagctac aaatagcatc
    12661 ataccaacta atacaccaat agtattgaaa tctatatggc ttaatccttc atcaagtgta
    12721 agtatattga atattatcat taatagtgca ccaccaacag ctgctacgga tctgtttacc
    12781 ttttctgtaa taatagctgc cataactatt aaaaatatac atatagctat tatttgttga
    12841 aattccataa tataagtacc tccatttgta ttattttgtg cctaatgcta gtactatata
    12901 tagtattact tttttattgt tttttcaatg gatgtatgaa agtatattat ttaacaaagt
    12961 ttttggaaaa ttgacagaat ttaaagaaaa tttttgttat ttaaataaat tttaaaatta
    13021 ttccattgat aataatatta aacgacaaac gaaaaaatat accctttaaa ctagaattta
    13081 ttgtatctaa taacaattat aaaatattaa atatatctat attataaaaa caataaaaaa
    13141 aggtttgatt tgaatataaa actgcaatat attaaaaaaa ttattcattt taaaccttgt
    13201 aatttgattt tataaatatt ataattaaat taatgaattt ttaacaagga attattcatt
    13261 tttttgattt taagatttat tgagaaaaca aaactaaata ggggggtaat taatataatg
    13321 gattttttca atggtatttt aacgagtatt aacaatgcag tagtatatgt aaacaattac
    13381 ttatggtcgt atatcttaat tataatgtta gttgtaattg gtatttactt tactattaaa
    13441 acaaattttg tacaattcag atattttata gaaatgttta gacttctggg tgatggtaca
    13501 gcgaataaaa atgcaaaaaa ggaaggaaaa atctcttcat ttcaagcatt ttgtataagt
    13561 acatcttcaa gagttggaac tggaaatata gctggaatag caattgcagt ggtagcagga
    13621 ggacctggtg ctgtattttg gatgtggctt atagcattaa ttggttctgc atcaagcttt
    13681 gttgagagta cattagctca gatttacaaa gtgaaagatg gtcaagcttt tagaggtgga
    13741 ccagcgtatt atatggaaca aggtctaaat aaaaaatgga tgggtatttt attctcaata
    13801 cttataacta tatgttatgg ttttgtattt aatgcagttc aagcaaatac tgtttcactt
    13861 gcatttaata atgcatttgg aataagtaaa atggctatgg gaataggtct tggaatttta
    13921 actgcaatag ttatttttgg tggagtgcat agagttgcaa aagtttctga aataatagtt
    13981 cctgtatttg caggattata tatattagtt gctttggcta tagttgtaat gaatataaca
    14041 gagataccaa gtgttatagc attgatagtt gaaagtgcat ttgattttaa aggaatggcg
    14101 attggtacat ttatgggagt tgtaatgact ggagtgaaga gaggtctatt ttctaatgaa
    14161 gcaggtatgg gtagtgctcc taatgctgct gcaacagcac atgtaactca tcctgtaaaa
    14221 caaggtttaa ttcaatcttt aggtgttttt acagatacta taataatatg tagttgtaca
    14281 gcattcatgg tattattata ttcaggttat agtagtagtg gagcaactgg aattgaatta
    14341 gctcaagaag ctttaactca tcatatagga ccagttggaa atatatttat agcagtttgt
    14401 atattcttgt ttgcatttag ttctatagtt ggtaattact attatggtga atcaaatatg
    14461 gagtttatga gtggaagtaa gactaaactt aatgtattta gagtttttgt tgtaggtatg
    14521 gttttatttg gttcacttac tcaagtggat gtagtttgga atcttgcaga cttatttatg
    14581 gcattgatgg cgataataaa cttaatagca atagcacttt taggaaaata tgcatttatt
    14641 gctttaaaag attatacaag tcaaaaaaaa tcaggaataa aagaccctgt gtttattgca
    14701 gatgaaatag aaggcctaga aaaggtatca gagtggcata gggaattatc tgaggaacat
    14761 ttaggataaa aaatagtata aaattataaa accattttga aagactagag tatgtatata
    14821 ctttatatga atataatatt gaattaattt atattactgg aatttttaat tccagtaata
    14881 tttttttatt atataattta gctttattaa aagtttttat tattaaaaat agcttattta
    14941 tcgtaaagtg aatgatattg ggttttaatt attatgaatg ttagtattta atttaaagac
    15001 ttaaataaat ttgccatttc tagcgcattc atagcacaat cataaccttt atttccagct
    15061 tttgtacctg ctctttcaat agcttgttca atattttctg tagttacgat tccaaatata
    15121 acaggtaatt cactgtctaa agatactttg gctatccctt tagagacttc actacataca
    15181 taatcataat gagaagtagc ccctcttata acacagccta aacagattat agcatcatac
    15241 ttaccacttt tagccatttt ttgagcaaca agaggaatct caaaagctcc aggtacccaa
    15301 actatttcta tattttctgg agaaacgtca tgtcttaaaa gacagtcttc agctcctgat
    15361 aaaagttttg atgttataaa ttcattaaaa cgagaattta ttatacctat ttttaaatct
    15421 tttccaataa gttttccttc atatatcatt tttacttcct ccaattttat ttatgttata
    15481 gatttatatt tataaatatc tttaattt
//
LOCUS       DAGSHT010000072        15363 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGSHT010000072 DAGSHT010000000
VERSION     DAGSHT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.074"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            196..1542
                     /locus_tag="MJB72_002010"
     CDS             196..1542
                     /locus_tag="MJB72_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY8263118.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGRGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            1569..2969
                     /locus_tag="MJB72_002011"
     CDS             1569..2969
                     /locus_tag="MJB72_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY8263119.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            3179..4615
                     /locus_tag="MJB72_002012"
     CDS             3179..4615
                     /locus_tag="MJB72_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY8263120.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            4959..5903
                     /locus_tag="MJB72_002013"
     CDS             4959..5903
                     /locus_tag="MJB72_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBY8263121.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            6072..7004
                     /locus_tag="MJB72_002014"
     CDS             6072..7004
                     /locus_tag="MJB72_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBY8263122.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            7109..7576
                     /locus_tag="MJB72_002015"
     CDS             7109..7576
                     /locus_tag="MJB72_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBY8263123.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(7820..8674)
                     /locus_tag="MJB72_002016"
     CDS             complement(7820..8674)
                     /locus_tag="MJB72_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBY8263124.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            8840..10804
                     /locus_tag="MJB72_002017"
     CDS             8840..10804
                     /locus_tag="MJB72_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8263125.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            10804..11265
                     /locus_tag="MJB72_002018"
     CDS             10804..11265
                     /locus_tag="MJB72_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263126.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            11268..11588
                     /locus_tag="MJB72_002019"
     CDS             11268..11588
                     /locus_tag="MJB72_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY8263127.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            11604..12704
                     /locus_tag="MJB72_002020"
     CDS             11604..12704
                     /locus_tag="MJB72_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY8263128.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            12824..>15363
                     /locus_tag="MJB72_002021"
     CDS             12824..>15363
                     /locus_tag="MJB72_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBY8263129.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDV"
BASE COUNT         6105 a         1600 c         2667 g         4991 t
ORIGIN      
        1 attaaaaaat taagaataac ttggtaatag tgaaaatata atgagattat aattgattta
       61 taatataact ataatgatat tgtaaaaatg aaaattgtcc tctcaaaatt cccaaagaga
      121 gtaaaagtca cttcagttat agtcaaaaaa atttttctgt gaaaagaatt tagatttgaa
      181 aaggaggaat taatcatgga aattgaaact aaaactccag attggattaa tgtactagaa
      241 ttaaacagta aaatagatat tactgggaaa tatgatgtaa gtaatatgat acaaaatatt
      301 gtctctgata agtcactaga aggttccatt atatattttc caaaaggtaa ttacttattt
      361 gagaaaggta ttaaaattag tcaacaaata acacttcaag gagattctta ttatggaaga
      421 ggtaatcaag tatcaaattt agataaaaaa caatcaatca taggaacaac caattttata
      481 acccgaggag tttctaatat gagtattatt acattgtcag gaacaagtca atgtatcaaa
      541 aatattaatt tttattatga tagtcatgat atagagaaaa taccgcctaa aaatgtatct
      601 gctattacag aatatggaga aacacaagga ctatctcatt ttgaacatct gtttatttct
      661 ggattttcag ggataggaat tgaaattcca tactattcaa ctggtaatga tattactgtt
      721 agttcatgtg gtcttggtat gaggcttgga gagaagtcaa tgctttctag tagcaagatt
      781 tacgaatgta aaaatggaat ggagattact actggtgtta gcttaaataa tgtgagaata
      841 gaagaaataa aagaaatagg tattaataat aaaggatttg gttttaacct gattatgaat
      901 cttatggttg atcaatgtgg atattgtgga tttatgtttg aaaaaatgtg ctatagccag
      961 ataactgcca gaattactag atgcggtcag tattataaaa gtgttgatta taatgcttat
     1021 gagaatatgg aagataggaa agaagaagct tattccattt tttatggaga tattttagaa
     1081 aattgcaata ttgtattgat gaatagaaat attgatacct tagaaagtga ttcacttaat
     1141 gaacataaga tatatgtaat caaggcaaat gaaacaaaca atgtaatgct aacatgttat
     1201 gctgaagcag ataactttat acaatgtgca aagggcaact tgctgttaga aaatggtgaa
     1261 aatacttata aattttataa tggtgagatt tgttctgtag gaggtgttca aatttctgat
     1321 atagatgaaa aagatttaat caaaataata gagaacactt tgtatataga tgataaaggt
     1381 aagtatttaa tggttcctag attaaaaaag aatactgtaa tatattcagt actagattct
     1441 acagaagaaa taaataaatt acatggtgga atatgggaac agattgatgt aaaagtatta
     1501 gctggggaat ccatgtacta ttataaaaag ataaaagatt aaattatcaa actaggaggt
     1561 agtgattaat ggaagagtct ataataatta aagatataag tggctggatt aatgtacttg
     1621 attatggaat ttgtaatgat gaaacaaaag atgtaagttt taagttgcaa gagattatgg
     1681 atgctgtacc agaagattct attatatatt ttccaaaagg aaaatactta tttgaaagtg
     1741 gtgttaagat taagaaacga ttaaccttat gtggagattc ttatatttta gcgaatgttc
     1801 ctaaaaatac tgctggagta acacagttta cttttaaagg aaagaaaaat cctaaaagtg
     1861 aaaatagagc aatcataaca gcaaaaagtg taagacactg tattaagaac atttgctttt
     1921 attcagatag ttgtgataga gatatgacat ctaatcatga acctccaaca gatggaaaac
     1981 ctagatttca tcataatgta attataaatt atagaaatat atctgctgtt atatgtgaag
     2041 ataaacaggg agaaccaggt cattatgaaa atctatcttt tagtggtttt tctggaattg
     2101 cacttgatat gcctaataat tctacagcaa atgatattac aatttttaca tccggacttg
     2161 gaattagtac aggggaaaat tcaacaattg ctaacagtaa ggtttggggc tgtgttgatg
     2221 gaatgataat ttctactgga acttttataa atggtatgag ggtagaagaa ataaaaaaag
     2281 ttggaattaa aaatattggt agaggtttaa attttattac aaatatcaca attgaccaat
     2341 gtggatattg tggagtttgg tttgattcta tatccaatgt tcagatatca ggaaatatca
     2401 caaggtgtgg gcagtactat tatgatgtaa gctatgataa ttatttaatg ttggataata
     2461 gagttaaaga agcatattct ttattctatg gtaatttatt ggagtcaagt aatatagttt
     2521 tatcaaatac caatgaagat aattgggaag atttcaaaga agataagcat aaggtgtatc
     2581 ttatagaagc acttaaaacc aagaatgtac tattaacatg caatatagat gctactgact
     2641 ggattgttaa gtcagaaaaa ggaaatttaa cgtacaataa tatgcgacaa actgttatat
     2701 tttacgatgg aaaactaagt agtatcaatg gaattggtat atctgatgaa gattatgggg
     2761 ataaagtcaa gataaaaaaa ggaaatatat atataaatag taatgataag gatattaaga
     2821 tgcataaacc agaggtaggt gacattatgt caacaattat taaagataaa agtattcttt
     2881 ctgctcatta tgggggaaca tgggagaaaa ttggtgaaaa aatagagttt ggaataactg
     2941 tacactatta taaattgata gagaaataag taaatacgta gtatttgaat agatgtattt
     3001 tattggagag ctaggactat agttttaata taaatggttt ggtaatagca tggattgaat
     3061 aatataaaaa taatataaaa aattaatttg attatgtaaa tttctatgaa gtagtaaaac
     3121 tgtgacttta gtcataggag gttcaaacta agtaaaaggg aaaggaatat ataatattat
     3181 ggaaggaaga actgggatta aagatggaag atggattaat gtagtagaaa gaggtattaa
     3241 aaatgatggt tcgtacaaca taggtcctgc tttgcaggat attattgatg aaatagaaac
     3301 agatttttgt gtcttatatt ttccaaaagg taaataccta tttacaagtg gtgttaaaat
     3361 taataaagaa attgtattgc aaggtgattc taattcatct acaggtacta cacaatttat
     3421 aactcgtgga gtagaaaata tgagtattat tacattgaca gggaagaaac aatgtattaa
     3481 aaatataaat ttctattctg atagttgtga gatacaggta aataaagaac caccgacaaa
     3541 aggtaatcct aaatatcatt atgagatgat tattaataag aatgaaaaga atgttgagat
     3601 gaataatgta tcagcaatcc tgtatgataa cagtaagaat atgattaaag gtctaggtca
     3661 ttatgaaaat atttatataa gtggtttttc gggtacagga attaggatac catattactc
     3721 aattgtaaat gatattacag tttcatcttg tgggctgggt attgatacag gaatagacac
     3781 aattatttct aatagtagaa tatctaagtg tcaaaatgga atgaggatta gaactggaac
     3841 tagtataaat aatgtaagga ttgaaaaaat acagaaagtt ggtcttataa gtagtcaagt
     3901 taaggggtat gaggggtatg gaagttataa aattaataat attacgattg accaatgtgg
     3961 gtattgtggc ttttcatttg attcaatgaa agatagttat ttttctggta ttataagaag
     4021 gtgtggtcag tattattata atacagatta tgacacatat ttaagtatta aagatagagt
     4081 agaagaagca tattctttat tttatggaaa ttattttaca gaatgtaata ttgatttagt
     4141 tagtgataat aaggataact gggatgatgg cttagaatat aaacataaag tttatgtatt
     4201 taaaaccctt caaataccaa aattaacatt gagatgcaat atagatgcct atgattatat
     4261 cgttaaatcg gtacatggag gaggtaattt aatgttaaaa aataacttta acaattatat
     4321 atttattgat tcatctaatc cagaagttat taatggaatt aattttaatt atcaaaagaa
     4381 tggaaacttg ttaaggttaa ataatggtaa attaagttat aaaaatggaa aggaagaaac
     4441 agctatattc agacctaatt atggtcttat tgtagcatca acagattcag aatgtgaaca
     4501 aatagaggct acctatggag aaaaatggga gaagcttggt tctgaagtaa ttggagaaga
     4561 aacggtatat tattataaac atcatgaacc agataaaaca aaccaaatac aataaaattt
     4621 gtattatata atgatataaa gttttaatat atagtgttta cctttaatgt aatattggta
     4681 tatttgtata gtattaaatt tgttaaaaag taaaattgta gggtgcttga aaatagcacc
     4741 ttttttagtt tcattataaa attagttttg aaagtttagg aataatgtat atttattttt
     4801 aaagaattat aaccttatta aggatatttt aaatgctaga aatgaatcag taaatcatac
     4861 tggaattatc ttaaatgttt tgattaaatt aagtatttag agctataatt atattaaaaa
     4921 tatattagaa cataattata ttactatgga ggttaaatat gatagtaagt gtgagtagaa
     4981 gaactgatat acctgctttc tatagtaagt ggttttttaa cagacttgaa gaaggttttg
     5041 tttatgtaat taatccaatg aatccaaaac aggttagtaa gatagagtta aatccacata
     5101 ctgttgattg ttttgtattt tggacaaagg atgtaactcc aatgttatat gatttaaata
     5161 aattaagaga ctataaatat tattttcact atactataac atcatatgga aaagaagtag
     5221 agacatgtat tttagataaa agaaaggtaa tagattcatt taaggaattg tctagaaaaa
     5281 taggcaaaga aagagtaatt ttaagatacg acccgatttt cttaagtgaa aagtacacag
     5341 tagattatca tatcaaagca tttgcgagtt tatgtaatca gcttgatgga tttacagaaa
     5401 aatgtataat aagttttatc gatttgtata agaaaacaaa atttaatact aaagcattac
     5461 atataaagcc aatagaaatt acagaaatag aaacattatc aaaagagatt tctaagattg
     5521 ctcataagca tgggataata cttgaaatgt gttctgaaga atataattta agtaaatttg
     5581 ggattggaaa aagtaagtgt attgatgata gattaatatc aaaaattata ggatgtgaag
     5641 tagaggtaaa aaaagatagt acacaaagag atatttgtgg atgtgttaaa agtgtcgata
     5701 taggacaata caatacttgc agacattatt gtctgtattg ttatgctaac tttaattatg
     5761 caaaagtaga agaaaactgt aggctttata aggaagataa taaattactc atagatactt
     5821 taagagatga tgcaaaaata agcattagag atatgaagac tattaaagta gataagaata
     5881 aacaaattag tatatttgat taaaataaat atactaatta ccttaatata ttaagaatat
     5941 agatactttt gataacaaaa ttaaacttaa tagatatttt ctatataaga aagatggtca
     6001 taatatgtca atttgaccat ctttttgata taattaaaac acaaataatt tagtaacaaa
     6061 ggaggcagaa cttgaattta ttaaaaaaat atcctatacc tattgcaagt ttgattttat
     6121 caatttttac tttaggtaac ttattacaat cttatagtga atcattacgt aatgtaattg
     6181 gatttatagg atttattttg tatgcaattt atatagtcaa aataatatta cttagaaagg
     6241 gtgttaaaga gcaattagag aaccctttaa tatcaagtac atttccaact ataacaatgg
     6301 caacaatgat atttgctaca tatataaaac cattatcttt agaattaagt attatgattt
     6361 ggagcatagg aattgtgggt catcttgtgt taatagtctt atttagtaag aagttcttag
     6421 ttaaattctc aataaaaacg gtatttccat cttggtatat agtatatgtt gggatagttg
     6481 cagctagtgt tacggcacca gcatttaatc aacttttgat tggcaaaatt gctttttgga
     6541 taggatttgt atcttatata gttcttatac cagtaatttt aaagagagta tggataatca
     6601 aggaaatgcc tgaaccatct ttaccaagta tagctatatt agctgcacca gggtcactat
     6661 tattgtctgg atatattaat tctttttcag ataaaaataa tgtagtatta tacttgttat
     6721 tcatattatc aattgtattt tatattatag ttttgggata tttaccgaaa ttattaaggt
     6781 tgccttttta tccgagtttt gctgcattta cattccctat ggcaataagt gcaatgggta
     6841 tgaaactaat gaatggttat ttaactaaat caggtcattt tataagttgg atttcatatg
     6901 cttctaagtt agaagagata attgctacag tgattatttt atatgtttta gttttgttta
     6961 ttatatatct tagtaaatct gaaactacat ctaaagctaa ataagaatta ttaaatatgt
     7021 gtatttaata ggaatattga tataaatata tgaagataag ataaaccata gaaacttaat
     7081 ttagcttaat tttattggag gaaaacaaat gaaaacaaaa gaagaatatt taaaagaagt
     7141 tagaaacata tcagaagaat actttagaaa aggagagttt ttttgctctg aagcagttct
     7201 ccagacaata aatgatgctt taggccaacc tttaagtcca gagataacta agttagcatc
     7261 aggatttccg ataggacttg gaaaggcaca atgtctttgt ggagcaatat ctgggggaga
     7321 gatggcttta ggtatagtat atggaagagt tcatggtgaa gctatgaatc caaagatgtt
     7381 tgagcatgct aaaggtttgc atgattatat taaaaaagag tatggtgcaa catgttgtag
     7441 agctattaca aagaaatggg atggtgataa ctttatgtct cccgaaagaa aagagcactg
     7501 tattaaaata acaggtcaag ttacagagtg ggttgcagat aagcttatag aagatgaaaa
     7561 acttaatatc aaatagtgta atattagttg gtgaatttat aaagttaaat ttacttatag
     7621 tataagatac ttgatattct cgtattgata gaaaaattta tagcatatgt atttaatgaa
     7681 atatacttat atttagagtg tggtttaatt tcataaatac atatgctatt attttaagta
     7741 tataagcaaa tttatttaaa taaatagaaa cagttaattt atatgtacaa aactatatat
     7801 gattagtgta aataaatacc tattttataa tactcaacat aaagacacca gacatgatta
     7861 gtattatacc aataaaaata gcaagccatt cttttttgtt cttcttttct ttaaaaaata
     7921 taatccctcc taaagtggct actatcacac aactctgaga gatagaaaaa cttattgtaa
     7981 ctcccaatgt attagtcgtt atgaacatac ctaaatttgc tattgaccac aatataccag
     8041 taattaaatt gaataaaaca ttttccctat atacaatctc tttagaaaaa tacttgacaa
     8101 taatagatga agttatcaac ataccaacag attgaggtaa tataattcta tatccttcaa
     8161 ttaaaaatat ttgattagtt acaacatata atgataaaaa tagagatgat aataatacaa
     8221 ctccaacaca ttttaacata tttttatctg tagatttttt agtttgataa tttgtgagaa
     8281 gtataccaag tattatacat aatattgata aagaaccaat cagtaacatt ttagaactgg
     8341 tccattcatt aaacaagatt acagctaata atgtcgtaaa agttaattgt gaaccatttg
     8401 aaattggcat tactttagat acactagcaa tttgaattgc tttaaactgc attaattgtc
     8461 ccaaactcca gaatatgcca gaaatcatac ttatacaaaa tgaaaaaaat gtaatttcag
     8521 gatgtataac cagagttatt atagtggaaa atataaacgc acttatggat gtacctaaaa
     8581 gctgttcatg aggagaactt ttctttagat ttgcaactat gggcatcaat ccccatccca
     8641 ttacaggaag taaagcaata attattgtca gcattaaact atttcctttc atattaaatt
     8701 tagacagctt ttaatttatc atctaaaatt aaagtgttca atacataaaa attaccgtat
     8761 ttaatcagga aatattagta gttttttaga aaaacatttt ccattaaaat aaagacaaag
     8821 ggaaggagta aaattgaaaa tgttaactga tatgattcaa aatcgtgaaa ttaaaataat
     8881 aaagttttta ttaaaagaag gtcatacaac tgtaaaagca atagcagatg atttagatgt
     8941 ttcagaaaaa acagtttcaa aatctcttaa agaagtagaa gcagtgctta aaaagatagg
     9001 tctttcttta ataagaaaac ctaagatagg tgtttatatt gatggagatt taaaaaaaat
     9061 acttccttac ctagataata aaggaagtca aattcctaca accagagagg agagagttat
     9121 ctatatatat tcagtacttt taaagagtaa tgattatatt actatacaac aactttctga
     9181 tgaactatat ataagtcgtg gaactattga aagggattta gcagaagttg aaaaactgct
     9241 aaaaaacgaa gggatttcca tatataagaa acctagtaag ggaataaagt taaatataag
     9301 tgaaagagat aaaagacaac ttactgctaa gtttatacat aaattttgga gtagaaactg
     9361 gtatattaag caagagcaag aaagctttta tcaagcattt gaaaagatac aagcagatgt
     9421 aaatggtatt ttttcggaag atgatttatc aataattatt gatattctta ggaatttcag
     9481 cagagaaaga aaatttgagt tctctgatta tgaatttcag tcattagtta ttcatttggc
     9541 aatagctgta gagagaatta gaaaaggcga atatatagaa gaaagtttgt atactggtaa
     9601 tatagaaaat aatcaaatgt caaataccaa ttatttagca aaacaattag aagaaaaatt
     9661 ttcaatatta ttaccaaaat ctgaaattgg atatataaat ttacatttag tagcgtctga
     9721 ccaacataga gatactaatg aactaaaaaa tacttcaact aatccttgtg agacaagtac
     9781 aaataatata tcaaatcttc gggaaattat aaaaaataca ttgtatgaga cagcttatga
     9841 caaggattta attgatggat taacttcaca tgtacattca tcaataaata gactgaaata
     9901 tggacttagt attaaaaatc catatgtaaa tacaatcaaa caaaactttt ctttatcatt
     9961 tgagcaagct ttagatttaa agaaagtaat tgaatttata tatgatatta ggataaatga
    10021 tgatgaatgt gcttatatag cattacattt tgaagctttt agagaaagaa tgaaagaact
    10081 tccacaaaat attagagtaa ttctagtatg cagtacagga ttaggttcat ctaggttatt
    10141 gtctgcaaga ataaaaaaat actttccaat gataaagatt gaaaaagtac tttccgtaca
    10201 agaattagta tccagtaaaa ttgatgcaga tttagtaata agtactattt atttagagtt
    10261 agaaacaatt ccaaccatag tagttagtcc tattttaaat gaaagtgata taagaacctt
    10321 ggaaagaaat atacaaaatg tttcaagtta tacttctaag aagtataaat ctttcagaaa
    10381 cttaatcttt gaagaaaata tattgttgaa tattgaagct tcaaactatg aagatgcaat
    10441 taagtatata acagataacc taataaaaaa aggttttgca aaagaaggta tagctaaaag
    10501 tgcaattgag agagaaaatt tatcttacac ttctttccaa tcaatagcta cacctcatgc
    10561 taatcctagt tatattaaaa aatctaatat atctgttgca acgttaaaaa atcctattac
    10621 atggggaaat gaaaaggtgg aagttatttt ctttatttca ttagaaaatg acaaaaacct
    10681 agagcttgat gaaatatatg aagtttttta tgacttcatt gacaataaaa aaaatataaa
    10741 tgacatatta agtgcaactt ccacaaagga agtgtacgat ttgctagtgg aggagagtat
    10801 atagtgaaaa tatcagatgt attgaaaaaa gagcaaattg tttttaatct tgatacaaat
    10861 acaaaggaag aaacaataaa aaaattaagt agtatatttg ttgaaaatgg gattgtggac
    10921 aatgaagaag aatatgttaa gtctgttttg gaaagagaag aacattcaac tacaggtatt
    10981 ggtaatggta ttgctattcc tcatggaaaa agtaatgctg tcaaaaaatc ttctataata
    11041 tttgcaaaga ctaagcataa gattgaatgg gatgctttag atgaaaaacc agtagatagt
    11101 attttcttat tggctataag caatattgat aaggatagca atcatcttgt actattatct
    11161 aaattagcaa ctaaacttat ggatgatgac aatgtagatg cactaaaatt agccaattca
    11221 gaacaagaaa taatcaatat atttagtgaa gaaggagaga tgtagaaatg aaaagaaaaa
    11281 ttatagcagt tacagcatgt gcaacaggag ttgcacatac atatatggca gctcaagcgt
    11341 taaagaaagc ttcaaaaaag atgggtcacc ttataaaagt tgaaacacaa ggagctacag
    11401 gaatcgataa tgagttaact acaaaggatg cacaaattgc agaagtagta atatttgcag
    11461 tagatactaa agtcagaaat gctgaacgtt ttgagggaaa agaaatacta gaagtaccag
    11521 ttaatgcacc tataaaggat gcagagaaag taataaatga tgcactaaag ttaattgaca
    11581 ctaaataaat ggagggaaat ataatgttga aaaagatagt ctctgagtta aaaaagcatg
    11641 tcttaactgg aatctcatat atgattccat tagtaattgc aggtgctatg ataatggcga
    11701 tatctcgtgt aggtggttct ttctataata taccagatat atgggatgct aaatatgcag
    11761 aaagtgctag tagtattgtt agattattac atgatttaga tgggtttgga ggtactgcat
    11821 taggattaat gtttcctgta attgctgcat ttataggatt ttctatagct gataaattag
    11881 ctattgtacc aggtcttgtt ggaggtatga ttgctaaaga tataggtgca ggatttttag
    11941 gagctttggc agttggtctt atagcaggat atacatgttt gtttattaaa aatcatgtca
    12001 aattaccaaa atcagcagct tctatagtac caatatttat agtaccagta tttgggacat
    12061 taatcactgt tgtattaatc aattatgtga taggtatacc ttttgccaca ttaaatactg
    12121 gattagaaaa ttggttaaat ggattatctg gtacaaatca gatattaatg gcagctatta
    12181 taggggctat gatggcagtt gacttaggtg gaccagttaa taaagcagct ttaacaacat
    12241 cacttgcatt attaacaagt ggaatttatg cgcctaacac tgctgcaatg gtaggaatag
    12301 ttataccacc actaggattg ggacttgcta ctatactagc aaaacaaaaa tataataaac
    12361 agttacgtga agctgggaaa tcatctctta taatgggttt aattggagta agtgaaggag
    12421 caattccttt tgcagtagaa tctccattaa aagtaatacc ttcatgtgta attggaacag
    12481 caattgcttc agctatggca gttggtttag gttcagtaaa tgcaactcca attagtggat
    12541 tttatggatg gtttacagta gagaattggc caatgtatgt gttatcaata gcagttggaa
    12601 cagtaattgt agctggatta gttatagtat tgcgtggaaa tccagtgatt gaggatgatg
    12661 aatttgaaga tgatgatttt gatgaagcag agtgggaatc ttaaaattta ataactattt
    12721 aagacgtgtt ataagctata ttcaatatga atcttatact aaataaagtg ttaaatattc
    12781 agtttagact taaatataaa agataaagaa cagaggagaa taaatgataa aagcacatat
    12841 tgttaatcat acacactggg atagagaatg gtattttact tctggagatg cattagtact
    12901 tagtgaacaa ctatttacag atgtaattga tgaattagaa cgtaatccag atgtaagttt
    12961 tgtacttgat ggacaacttt ctattttgga tgattatgta caattacaca aagaaaaaat
    13021 agaaatcatt aaaaagttaa taaaagaaga tagactacat attggaccat ggtttactca
    13081 aactgatgca ttctttgcta gaggagagtc tattttaaga aacttaatga ttggaatttt
    13141 tgaaagtaaa aagtatggta agtatatgaa aattgggtat ttgccagata catttggatt
    13201 taatgcacag atgcctatac tattaaagca tgtaggtctt gataatatat tcttttggag
    13261 aggtatacat cttggtaaac aggttcaatc accatatttt aagtggaaaa gcttaaatgg
    13321 agaaagttat atatatgcag taaatatgcc acatggatat ggaactggta tgcttttaga
    13381 gccttcatta aagtatgtta atggacgctt agacccagct attgatttta ttaaatcata
    13441 tactgatgta gatgaggtgt taataccttc tgggaatgac cagttaaata taataggtga
    13501 tttcaaaaat aaaattaaag cgattaatga tatcggaaag tattcatata taacgagttc
    13561 atatcaagag tttttaaaat atataaagtc catagaatca ctagaaagct atagaggtga
    13621 atttagagaa cctgtattag caagaataca taaatcaatt ggttctagta gaatggatat
    13681 aaaattagca tgtgataaac tagaaaacaa gctgattaaa agaatagagc cattattagt
    13741 aattgctaaa aagagtaaaa ttgaaataag caatcagtta cttataaata catggaaaaa
    13801 acttttggaa ggacaagcac acgatagttt agctggatgt gtaactgata ctgtaacaga
    13861 tgatattctt caccgtataa gagaagcaaa tgaaatatgt gacagtattg aaaatactat
    13921 tgtaaaaaag atttcagaag ggcttaaatt atctaaaaat gatattttag tatttaatac
    13981 agaagctaag cgttttaatg gatataaaga gatacaagtt gtaagtgata gtaaaaatat
    14041 ttattttaaa gataatttag atgctactat aattgaagaa acttatgtaa aaccgagaga
    14101 aaatgtatta gaagaaacac ctgctggtaa tatatttatt gaagaaccag gatattatat
    14161 attaaaagta agaataaata tacagcttcc tgcacttggt tataaagttg ttagttttga
    14221 attaagtgat aaagaaatgg tgtcacttga taaatctaat gacactttta tatctaatga
    14281 taattataag ataatatatg aagatggaag cttaaattta caacttaaag atggaactta
    14341 catatcaaac tttttaacat taaaagatag tggtaatgca ggtgatacct atgacttttc
    14401 tccactaaaa aatgatgagg atataaaact ttcgtttgat aaagtgtata cagaaaaagc
    14461 tttaggatat gaaaaaatgg tggttaaagg tactacttta ctaccactta cattagaaga
    14521 tagaaaaaac aaatatctta atgggaaatt aaatgtaaaa gtaagtattg tcttatctaa
    14581 agaaaaccca ttgatggatg taaaactttg tgttgataac acgatatata atcatagact
    14641 acgtgttcat ataaaaactg atataaaaga taataaaaac atagcatcat tgccatttgg
    14701 atatataaca agaaaaaatg gagttttaga caactgggaa gatatttaca gtgaaatgcc
    14761 tatagattta gaacctttgg aaagtaatat tactctaaca aaccaaaata gaagttgtac
    14821 tgtatttact agaggaataa aagagtatca acatattgaa gatgaaattg cattgacctt
    14881 acttgctact acagatgaac taggaaagcc tgatttatta tatagaccag gacgtgcttc
    14941 tggtgataca actaaaaaag gtcatataag aattaaaaca gataaagctc aaatgcttaa
    15001 ggaattagaa ttttcatttg caatatatat ggattctaaa tcatttgatg aattggaaat
    15061 tgcgaattta acccacaatt atctaaggga atgtgtaaat tatcaattac aggattacaa
    15121 tttcttttta tatcgtatag ataataagat tcaaaaatca attgtcaata aaactgtaag
    15181 tagagaatta gaaataatat ctttaccaga aaactatctt atatcagcat gttatccttc
    15241 ttattacagt aaagataaat ttataataag agtggaaaat ccaagtagta atagaatgat
    15301 attggatgag caaatattta aaaatagaaa tgggaaaatt gtaaatgcaa tagaagatgt
    15361 aga
//
LOCUS       DAGSHT010000073        15250 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGSHT010000073 DAGSHT010000000
VERSION     DAGSHT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.075"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            170..1561
                     /locus_tag="MJB72_002022"
     CDS             170..1561
                     /locus_tag="MJB72_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY8263130.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1814..3607
                     /locus_tag="MJB72_002023"
     CDS             1814..3607
                     /locus_tag="MJB72_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY8263131.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3688..5070)
                     /locus_tag="MJB72_002024"
     CDS             complement(3688..5070)
                     /locus_tag="MJB72_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY8263132.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5328..5675
                     /locus_tag="MJB72_002025"
     CDS             5328..5675
                     /locus_tag="MJB72_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY8263133.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5693..6727
                     /locus_tag="MJB72_002026"
     CDS             5693..6727
                     /locus_tag="MJB72_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263134.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6776..7825)
                     /locus_tag="MJB72_002027"
     CDS             complement(6776..7825)
                     /locus_tag="MJB72_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8263135.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8100..9635
                     /locus_tag="MJB72_002028"
     CDS             8100..9635
                     /locus_tag="MJB72_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263136.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9820..10506)
                     /locus_tag="MJB72_002029"
     CDS             complement(9820..10506)
                     /locus_tag="MJB72_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBY8263137.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10631..12013
                     /locus_tag="MJB72_002030"
     CDS             10631..12013
                     /locus_tag="MJB72_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY8263138.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12415..13683
                     /gene="eam"
                     /locus_tag="MJB72_002031"
     CDS             12415..13683
                     /gene="eam"
                     /locus_tag="MJB72_002031"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBY8263139.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13776..14132)
                     /locus_tag="MJB72_002032"
     CDS             complement(13776..14132)
                     /locus_tag="MJB72_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263140.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14283..>15250)
                     /locus_tag="MJB72_002033"
     CDS             complement(14283..>15250)
                     /locus_tag="MJB72_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY8263141.1"
                     /translation="FIIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISF
                     ILFTVPSIVNSLADLVAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPK
                     FSNLLIGSLGGIFSTTFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKK
                     YGDFIIELCQSLNLNIGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNM
                     VPYFGPVIGMAPVVIINLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFF
                     TILAVTVGGGFFGIPGMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5897 a         1892 c         2472 g         4989 t
ORIGIN      
        1 taaaaaaata atatttttca taattttaaa aataataata aaaaaatgaa aatacaggct
       61 ataatctatt gagtaaacat agaaaagatg atattataag tatgtgctaa aatggttaaa
      121 gcaatattaa tattaaattt aactataaaa caaaaggggg cgttttttta tgtcaagtac
      181 agaaagcgta tctaaaaaaa gtggtcatcc gaagagtttt tggcttattt gcatgacaat
      241 tttgtgggaa agatttagtt attatggttt aacagcttta ttggtattat tttttacagc
      301 aagtgtagct caaggtggtt taggtctttc tactcaagaa gcaacatcta tgtttggctt
      361 atttacaggt cttatatatc ttacaccatt agtcggtggc tggttagcag ataaagtttt
      421 aggtcaacaa aaatgtattt ctataggtgc acttttaata gcaataggag atttcgtact
      481 atttgcttca tctacatcta ttgcaatggt ttgggtaggt ctatcaataa ttattttagg
      541 aaatggtttt tttaaatcaa gttgctcaaa tatagttggg gatatatatc ctaaaactga
      601 tgtgtcaaga aaagatgcag catacagttt attctatatg gcagtaaatg taggagcatt
      661 tatagctcca ataatatgtg gattgctttc tgataatgta tttgcagtaa gaggagcaag
      721 tggagatata gtatcttatg gatataagat ggcattttta atttgtggta taggcatgat
      781 gttaggtatg attatattta cacttcttgc accaaaatta ttaaatgaag tcggaaaata
      841 tcctgtgact aaaggtaata aaggtgaaaa aattgagtat ggaccactta ctaagttgga
      901 gaaaaatagg ataatagcaa tggttatttt atttttattt gtggtcttat tctggacagc
      961 atactatcaa acacaaagtt cttttatgat ttatgtaagg gataatgtaa atagaactct
     1021 atttggattt gaaatgccag tagcatggct tacatcacta aatgctatat tatgtgtggc
     1081 tttatgccca gtattagcta ccctatgggt taaattatct cataccaaaa gaggtgattt
     1141 ctctattcca gttaaaatgg gattaggaat gataataatg ggtgttgggt ttatagttat
     1201 ggtatttgct acattagcta atggtccaga tggaatggta aaggctagta tattgtttat
     1261 agtattgact tatatattta caacagtagg tgaattattc ttatctccta taggacttgc
     1321 aatgttcaat aaattagctc cagctaagta tgctacgttg tctatgggag cctggtatct
     1381 aacaagtttt tttgcaagtt tattatcagg aaagattgct ggttttacag ccacacttgg
     1441 ttttttacag atatttggag gaatagcaac agttttaata atatttggtt taatattatt
     1501 gggaataaga aatgtattaa caagattaat ggctatggat ttactaacaa aagaaaaata
     1561 attcatgacg aattattgta aataaatgaa ttactttaaa taaaggaagt tacatttaaa
     1621 tataaaatta aatgtaattt cctctttgat tatgaaaaaa agctttacac aaatagatat
     1681 tataattaaa attataattg tgataattag agtgtataag atttatataa attatacaaa
     1741 aaatatttat gtaataaact ttacatactt atatatttag ttattcaaac tattatttcg
     1801 aggaggtctt gttgtgaata taaaagatag attaagtgga ttaagaaaat ttatggaaga
     1861 aaaaaatata gatgcctata tgattccatc ttctgataat caccaaagtg aatatgtagg
     1921 agattacttc aaatcaagag aatttatatc agggtttaat ggttcagcag ggacggttat
     1981 tgtgactaaa gacgaagctg ggttatggac agatggaaga tattttatac aggcagaatc
     2041 tcaattagaa ggaagtacaa taaaattatt caaaatggga caagaaggtt gcccaactac
     2101 agatgaatat ttatataaaa atattcctga aggaggaact ttaggatttg atggtagagt
     2161 tatatctgct agagaaggag ctacattagc tgaaaaatta tctaaaaaag gtataaagat
     2221 agaatatcag tatgatttaa tagatggtat atggccagat agacctgctt tatcagatag
     2281 taaagcattt ttattagatg ttaaatattg tggagaaagt tttagctcaa aattagctag
     2341 attaagagaa aaaatgagtg aaaaaggtac ttctactcat gtaataacta ctcttgatga
     2401 tatagcatgg ttattcaata taagaggtgg agatgttaaa tataatccag ttgtactttc
     2461 ttatgcagta ataactttaa aagaagttta tctatttgtt gatgaaagta agttaaatga
     2521 agaaatacta aatgaactag ctaaggaaaa tgttcaaata aagccatata atgatgtgta
     2581 tgaatttgta aaaaatatag acaaaactga aaaagtttta ttagatggaa ctaagcttag
     2641 ttatacaatt tacaataata taccttgtga agttgagaaa gttgatgagt tcaatccagt
     2701 aatgtttttt aaagctcaaa aaaatgaggt agaattagaa aatataagaa attcacatgt
     2761 aaaagatggt gtagctttca ctaaatttat gtactggtta aagaaaaatg taggtaagat
     2821 ggaaatcact gaaataagtg ctactcaaaa attagaggac ttaagaagag agcaagaagg
     2881 tttctttgag ccaagtttca atacaattgc tgcatataaa gagcatgctg caatgatgca
     2941 ctatagtgca actccagagt caaattataa attagaggca gaaggattat ttttagtaga
     3001 ttcaggtgga caatattatg atggaactac tgatataact aggactacag ttttaggacc
     3061 tataagtgat gaattaaaac tacactttac ttctgtagca agaggaatga taaatctttc
     3121 taaggctaag ttcttgcatg gttgtagagg atataactta gacatactat ctagaagttg
     3181 catgtggaat atgggaattg attatcaatg tggaactgga catggtatag gatttgtgct
     3241 aaatgttcac gaagctccaa atggatttag atggagagta gtacctgaaa gatttgatag
     3301 tgcagttctt gaagaaggaa tggtaactac taatgaacca ggtatatata tagaaggttc
     3361 tcatggtata agaacagaga atgaaattgt agttagaaaa gctgaaaaga atttctatgg
     3421 tcaatttatg gaatttgaag ttgttacgtt agctcctatt gacttagatg gtatagtacc
     3481 agaattaatg aataaggatg aaaaagatta tttaaactgg tatcataagt tagtttatga
     3541 taaaatatca cctttcttaa ctgatgaaga aagagaatgg ttaaaagttt acactagagc
     3601 tatatagttt gtactagata caataaaaat cgaggtatta taatgcctcg attttttatt
     3661 gtatatttta ttaactttaa aaaattttta atttgataat tctactaatt ttttattcat
     3721 tataagtagt ataattccaa atatcaataa aaatgatggt atcactatga agacttgtaa
     3781 cataccaagt tggtctataa tagcttgcgt atagccagat aatttatttg ctgcaaattt
     3841 gcctaaaaac caaacaccca ttaggaatga aatatacttt tcaggcgaaa gtttatttac
     3901 cataccatac ccgactggag aaaagcaaat ttctcctata ctttggaatg ttaaaaatcc
     3961 aactacccaa aatacacttg ctttgtgaat tgaattaggg tctgttcctg tttgtataac
     4021 tgctactatc ataaacaaaa atgctatggc taatagtata aaacctaagc tcattttttt
     4081 tggcacactt aaatcgcctt ttttagtttg agataatttt acccatacta aagcagagat
     4141 aggtccaaga ataacacaaa ggagaccatt gtatgaatct atccatgaag taggaatttg
     4201 aaaattgcca acagtaagat ttatgtgttc ctttgtatag agtgcaatag acatagaggc
     4261 ttggttatat gctatccaaa aaaatactgt gaaaaaagct aacaccaata ttactattat
     4321 tctttttttc tcaatcaaag ttaaaggtct tgaagcgagc attttcttat attctagtga
     4381 ttttcttata gactctctcc cgttaatagg gtcattattc aaaggtttat aaaactttct
     4441 gtttccaaat ataaagaaga ataaacctat tagaaccgta attgatgaaa ataaaaatac
     4501 atatctatat ccatacatac ttatagataa tccgcttttc atagcaaacc attcattagc
     4561 aactaaaccg catattaaag ggccaaataa agtacctaga ttagtagcca tatacgtaat
     4621 agagaaagct ccatcttttc ttgggtcatc attggtatat aagtttccaa ccatagtatg
     4681 taagtttcct ttataaaaac ctattcctaa tccagataaa aatatcatga tgtttatatc
     4741 catcttacta tttgctatgt atcctagaaa ataaccaatt gaaaccatta gcattccaag
     4801 tataatacag tttttgggac ctaaaaatct atcactgacc catccaccaa taactggaga
     4861 gaaataagtc catgctaaaa aagatgcaag cattgatgcg gattctgctg gtgttaaccc
     4921 taaaccacct ttggagactt gttcagctaa aaacaataat aataccaatt tcataccata
     4981 gtatgcaagt cctacaaagg taaatattga acagcagaaa tataaatctc taggttgttt
     5041 aagtttttct tgcgttttta aaactgacat atgtcgagaa cctccagata aatagtttaa
     5101 caaaatttta ccatactata tgagtatatg tttttttcat atagacgtca acttgtaaga
     5161 taatttaatt aagtcgaaat atatgtaaaa atagtatcat ttttgtgtat tcagtaatat
     5221 ttttttatga taatatatgt tattattata ttaaaataca aacatattta tggaaaggat
     5281 atgccaattt agtagttaga ttgattgttt ggcaggtggg atgagctatg ataagaaaga
     5341 aatctgaatt acaagtagaa gtaaaagaaa atttaaatgg tggtatagga aaggttaagc
     5401 ttgaaaacat aatacaaaat gaagagctta aaggaaaagg aagactattt aaaagagtag
     5461 ctttaccagt aggaagttca attggtgtgc atgaccatac aacagatttt gaagtatatt
     5521 atatactaaa gggaaaaggt aaagtatttg ataatgggga atttgtagaa gtaaatgagg
     5581 gtgatgtagt gtatacagct gatggcgaaa aacattctat agaaaatata ggagaagaag
     5641 atcttgagtt tgtagcacta gttttatata tgtaaaatat ggaggaggag tcatgacagg
     5701 caagatagaa gaaattaagg acaagataaa tagattttgc aactccaatt taaatcaaga
     5761 atacaaagat ataagcttta aaattttgca aaacctttta gataataata aagagataat
     5821 tcattcttct agagctgata tatggtcttc agctatacta aatatagttt tagaacaaaa
     5881 tcttttatac aataagaagc atccactaca tataacaaaa aaagagtttt caaaacaaat
     5941 aggtgtttct ttaaatacta taaataataa atcgtcttta ataaaagatg tttgtagtat
     6001 tgacttttta aatcaagata ctatagctat ttctaatgta gaattttggg taaaagagac
     6061 taattccaaa tcaaagtctg tagatttaga tttagaaaag aagaaaatac tatataaaag
     6121 atatattaag ctatcacaag atgcgaatag tgatattgaa tcaattagat atatggagga
     6181 agctgtaaat ataggtaaaa gtatgattac taaagaagat aaagaacttg gattttggaa
     6241 aggtttatct accagagcct atatgatagc acttgaatcc cttgctagaa agttagaaag
     6301 tattaataat ctaaaggagt ccaaaaaagt attagaatat ttaatagaaa ttaatcctag
     6361 tgatgagcag ggaataagat acaaattatt aaatgtatta attagattaa atgatagaac
     6421 ttcaataaat aaattatttg aactttataa agaagaaaaa tctgctacat ggatgtacag
     6481 taaagctctt tattacttta aaaataaaag tatgtttttg gcaaatgatt ctattaaggt
     6541 tgctagaagt aaaaataagt acgttggatt atatttgtta gattggcgaa atgcttttgg
     6601 tagagaattt gttacagagg aagaaaaggc agaagctgtc tattactatg atgaaaacat
     6661 agtgatttgg aatgaagtta aaggttctat ggattggcta ttaaaaaaaa tgttggaatt
     6721 ttcataaaaa aaactactcc ttacttttat ataagtaaaa ggagtagttt ttaatttata
     6781 catctagttt taaatcttca ggttttatta gacctttttt tctcataaat tctgaaatca
     6841 acaaagttac aatagcagga agtataaaat taagtaaaag tataccaacc cacatagata
     6901 tacctccttt tccagcactt tgcatagcag ttactgttcc aaattgtcca accaatccac
     6961 aagtacccat accagaacca ataggaatat tctccatttt gaatattgtt gtagataatg
     7021 gtccaagtaa tattgaagct acagtaggtg gtatccaaat tttccaattt tttattatat
     7081 taggtacttg caacatagat gtcccaagac cttgtgccac aaggccacca actccatttt
     7141 ctctaaagct cataacagca aagcctacca tttggcaaca acaacctaca gttgctgcac
     7201 caccagcaag accacctaaa cttaacatca tacaaatagc tgcactactt ataggtaatg
     7261 taagagctat tccaacaagt gcagagacta aagctcccat tattataggt tgcatagttg
     7321 tagcttgcat tattatattt ccaaatgaag tcataaaagc agaaacagct ggaccaataa
     7381 gttcagctac aagtactcca attagtattg tcacagcagg tgtaaccaat atatctattt
     7441 ttgtttcttt tgaaactaat tttccaacct ctacagcaaa aagtgtagcg acaaatgctc
     7501 caacaggtcc tcctaattca ttaccacaag ctccaacaag tgcacaagaa aatagtacaa
     7561 gtggaggtgc ttgaagtcca tacgcaatag ccactgctat agctggacca gtaacctgtt
     7621 tagcaagagg gcttataact tcagtaaaaa gagaaatatt gaaattatct cctaatgtat
     7681 tgaaaattgt tccaattaaa agggacgaaa aaagaccgag tgccatataa cttagtgcgt
     7741 caataagata cctttgaaca gaaatttcaa tattttttct tttagcaaat gatttgaaat
     7801 ttcttttttt ataattgatt tccaatttaa aatcctcctc atataatata agtataattt
     7861 ctttttaatt aattatataa cttttcagct gacaagacaa atgttatttc actaaaatta
     7921 gactattgta atagtaagaa attaacatta attttagttt taaattttaa atttatggtt
     7981 tataattagt atagggatat tctgaaatat aataattttc cagtttatcc caatatttaa
     8041 cttaggtaga ttgcttacaa aacaaatgat tatatattag gctaaaagga agtggataga
     8101 tgatatttgt aaaaaataag aaatacagaa aaattgccat aattatatct attcctatat
     8161 tgcttgttgc gcagtactac ctttttaaat ttggaatact tagaccaatg gtaaagggag
     8221 tagaaattga aattgtagat ggggaatatg ttaaagatat agataaatat gtagtaaaat
     8281 taaatgaaac agttactttg tctacaggtg aatatattaa ggttccttcg tatgctaaaa
     8341 atccaaatat ttggtttaat gtattggata actcagggac tttaaaaata gatggaaata
     8401 aaatgacagc attaaaggtt gggtattctt ctgtgggtat catgaaaaat tcaagagtat
     8461 taaaaaaggc tactataaaa gtagtagacc cagaggtaga atcactagat atggagatta
     8521 atggggattt aaaacatgtt ggtgatagtg ctactataga aagtacaata gaagttgatt
     8581 atgataagtt taaaaaatca tataagccag tttataagag tagcaatgaa aatattttaa
     8641 aaatcaaagg aaataaagta agtgcagttg gagttggtaa agctacaata tatgctaagt
     8701 gtggaaataa agaaattgag agaacattta ggattcaagc tagagttgct gaaataaata
     8761 tggatgatat aaaaatagaa gttgaccaga atgaaaaatt gaaaccagat ataataactt
     8821 ctccacctgg tttagaacca ccaaaaatat attatgagtt agtagattca aaattacctg
     8881 tagaaagagc aataagttta agtagtaatg gaaatgttgt agggcttaga gaaggtaaag
     8941 atagggtgag aataacttgt ggagataaat ctaaagtaat aacagtaaca gtagtcaaag
     9001 aaactataac gaatgactat atacaaaact tggttggttc gcataaaata gtaaacaata
     9061 aagttattat taaacttgaa tgggattata tgaaagatgt ctttgattat gatatatatc
     9121 ttaaagataa gttgtcagga gaatcattta agaaaattaa atctataact ataaatgaaa
     9181 gtgatttagg taagtctaac aaggtatatg cgactataga agtagatatg aagggtaatc
     9241 aaaatataga ttttgatatt tatgtcatag gtaaaaagga ttcagataca actaagccaa
     9301 gtaatgtaat aaatattacc aatggaaatg gaaattcaaa taatgataat aatattaaag
     9361 atatgagggt agaaaatata tcagctaatg ttgatagaga taataatgtc attagagtta
     9421 actggagtag tatatctcat aaggactgta cttacagtat atatgtaaaa aataatacta
     9481 atggagatgg agaatttact ctatatcaaa gtgatataca gggaaatgag catactataa
     9541 gtattcctga aggagattta aatctagata tttatgttgt tgcaaattat aatggaaata
     9601 gctctcaaaa tagtgatgtt ataaatgtaa gatagatttg tatacaaatt gaaagtgttg
     9661 agtttattta aatattaaat taagaaaaag gatagtatag ggtaattata ctatcctttt
     9721 ttgttacaat atataggttt aaaatctcta gtaatataaa ttataaaaag tcttatttac
     9781 acaaatctat agatattttt tttattaatc aatattgtct taaattgaac ttagtgcagc
     9841 ttctattgca tgtattactg tcgtatcaaa tagtggaata gaagtgtcat tttgtttaat
     9901 taaaagacct atttctgtac atcctaatat aacagcctct gcaccttgtt tgccaagctt
     9961 atctataata gataagaaag ctttttttga agattcagat ataattccta agcaaagttc
    10021 attaaatatt gtgtcattta ctattatacg gtcttcttca tttggaatta agacttcaat
    10081 tccattatta ataattacat ttttataaaa atcttgttcc attgtatatt tagtacctaa
    10141 taaggccact ttttttattt ctttttccct taataccgta gcagttacat ctgctatgtg
    10201 aagtaatgga atatgtatac tttcttgtat tttatctgat accttatgca tagtattagt
    10261 acagataaca ataaaatctg cccctgcctc ttgtaattta attgcagcat cagctaaaat
    10321 tttggcgctt ttttcccaat ttccactaga ttggcattct tcaatttctt gaaaatctac
    10381 actgtaaagt atacatttag aagagtgtag tccacctaat ctttctttta ttactgtatt
    10441 aataacttga taatatgtaa tggtactttc ccaactcata ccaccaatta agcctatagt
    10501 cttcatatta tacctctctt atttatttat tttgaaatag ctatttacaa atacaagtat
    10561 agcatctaaa atagaagcat acaataataa aattgtatgg agtacaatta taattaagag
    10621 gagtaattat atgccaataa attcttttga aaactatcct atgacatgga aaccaaagtt
    10681 agataaccga aaaccaccaa tatataaaac tttagcaatg ttattagaag aagatattaa
    10741 aagaggaaat ttaaatcctg gagataaact gcctccacaa cgggaattag cagattttct
    10801 agatttaaac ttaagtacaa taacacgagc atttaaactt tgcgaagaaa agggactaat
    10861 atgtgcaaaa gtgggaaagg gaacattcat ttcttctgat gtaaatgtaa gtaatacatt
    10921 attatatcaa acggaatcaa aagacataat tgagttgggg acagttcatc ctccgtatga
    10981 gcaaaataca tatattattg actttattaa aaatgtacta aaacaaccag aaatggatag
    11041 gtttttacaa tacatgtctc catctggcac atatatgcaa aaaaagtcaa ttgcaaaatg
    11101 gatagaaaga aataatgtat acaccagtga agaaaatata ttattatcta caggagggca
    11161 aaatgctata tgtgcaactt tactaggatt atttaaagct ggtgatagaa ttgctacaga
    11221 ttctttaagt ttttctggaa ttaagtcaat agctaaaatg ataggtattc aattagtccc
    11281 aattcctcaa gaaaataatg aaatatcgat tgaatatctt gaaagttatt gtaaaaatga
    11341 aaatataaaa ggcatatatc ttataccaga ttatcataat ccaacgacac atactatgtc
    11401 tgatttatct cgtaaaaaga tagccaagat agcaaagcaa tacaatataa taattattga
    11461 agatgctata aatagcattt ttagagacgg tatacaaact cccatatttt ctttagctag
    11521 tgataatact atatatattt ttagtacatc taaattttta tgtgctggtc ttagagtagc
    11581 atttgtagtt gctccaaaac gatatataga aaatttagaa aatgcactat ataatatgaa
    11641 tctaatggtt tctccattta cagcagaaat agtacaccgt ttattatatt ctcctattat
    11701 agataaaatt atagaagaga aaagagatgc aattatagaa agaaatgaag ttgctgataa
    11761 agttttgtca gattataatt tgattggtga taagaattgc agttttcgtt ggttacttct
    11821 tcctgatgaa ttggatggaa aatcatttga aatttctgca aaaaatctag gagttcaagt
    11881 atattgtgct gaacgatttt cagtaggaaa ctctacagtt ccaaaagcag ttcgtatttg
    11941 tgtaacagct ccaaaagatg tagaagaatt agaaaaaggg cttaatataa taaagtcact
    12001 tcttaagagt taaatttatt ttactattat ttaattaaat aaataaaaag gcggagaaat
    12061 ttattataat aaacctccgc ttttattatt taatgaaata ctttactttt tattaaaaaa
    12121 tttaatttta ataatcttga aaatataatc gttttatggt aaaataattc ttaaaaatag
    12181 aaaaaattaa tcatttgtta aaaaaaatgc ataatttatt gtaatttatt gacgaaggtt
    12241 gaaaaatatt cataaatgtg cgaaataaca gaaaaaatgc aaaaattaat tcaatttcat
    12301 ttgttataaa atctatatgg caaaattaaa tctgcctatc agaatgatat aaacacatca
    12361 gaaaggagga cgctatcaaa taatacaagt agttaacttt gatagcaaaa aattatgaat
    12421 gaacaaacta gaatatcctt agagagagct gctgaattaa aatcaaaaat tgatgattat
    12481 attcaggcta gaaaaacgat taacagaggt cttgaaaaag aagaagagat aaataaacga
    12541 aaacagaaaa tattaagtat cttaaatgga actgaagagg attggaataa ctacaaatgg
    12601 caattatcaa atagaataac agatgtagat actttatcaa aaattataac tctaactaaa
    12661 aaagaaaaag aatatataaa agaggttggt actcaattta gatgggcaat atctccatat
    12721 tatttgagtc ttatagaccc agaagatata tgtgacccaa taaaattact gtctatacca
    12781 acacatatag agttggaaga tgagcaagaa gatttggacc caatgggaga agagtataca
    12841 aacccagcag gatgtataac tagaagatac ccggatagat taataataaa tgtaacaaat
    12901 gagtgtgcta tgtattgtag acactgtcag agaagaagaa atattggaca acaagattct
    12961 cataagtcaa aagctattat ccaagaatct atagactata tcagagaaaa tgaagaaata
    13021 agagatgtac tagtaactgg tggagatgct cttacattaa aagatgatta tttagagtgg
    13081 attcttagcc aacttaaaga gataccacat gttgattatg ttagattagg tactaggact
    13141 cttgttacaa tgccacaaag aattacagat gaattttgca atatgctaaa aaaatatcac
    13201 cctatatata taaatactca ttttaatcat ccaatggaaa taactaagga atctaaagaa
    13261 gcttgtgaaa agttagcaaa tgcaggcgtt ccattaggaa atcaggcagt attattaaat
    13321 ggaataaata atgataaatt tgtaatgaga tgtttaaatc aagaattact gaaaataaga
    13381 gtaaaacctt attatatatt ccaaagtaaa catgtaaagg gaacaaaaca tttcaataca
    13441 tcagtagatg atggtcttga aatcatggag tatttaagag gatatacatc aggaatggct
    13501 ataccaacat atatagtaaa tgctccaaaa ggaggaggaa agactccttt gcttcctcaa
    13561 taccttgtat caaaaggaac agattacgtt atgcttagaa catgggaagg aaaagttata
    13621 aaaatggaag atgaacctgc tgtagatata aagaaactta taaaagaaca agcacaggat
    13681 taaaaaaata tttaaagttt ataaaaagaa acaactttaa aataaaaaga aaatggatgt
    13741 caaatttata tcaatttgca tccatttttt attagttatt tattattttg ttgttgatta
    13801 actaattcaa gatatccact tgggtggaag taatatatct cagaagtatt ttcatttaca
    13861 aagtaaccaa tatcaccatc tgcatctggt aaaaatacat aaccattaag atttttagct
    13921 tttaatgctt caaaatcact agcatttcct tgatatatgt aatcaacttc attgttgtat
    13981 tttactaaaa gttctttagc ttgttcttga gtcaatttag tattttggct tacaggaaca
    14041 ttcttttgag aatctgcata agaagattca tttactggtg cagttaataa caaacaagat
    14101 aaaactaata aagatataag tgcttttttc atactaaaac cctctttcat gtttaattat
    14161 ataagattca ttacttaata atattgtatg tttcctataa ttaaatatta ccattaaaga
    14221 gaataatatt acagttacac agctgtaact tttgattaca aaatcgtaat taaattgtat
    14281 ttttattcca tttgtatttt atcatgtcta cgatttacaa actcacagaa gtatatcttt
    14341 ataacgccca tcacaggagc agatagaatc ataccaggta taccaaagaa tcctccacca
    14401 acagtaactg ctaggattgt aaagaatggg ctaagaccta gttgtccacc tacgattttt
    14461 ggctctataa atgtaacttc aacttgttga attattatta agtaaattaa agaagttaaa
    14521 gcaattgttg gattggagaa taaattaatt attacaactg gtgccatacc tattactgga
    14581 ccaaaatatg gaaccatatt catgacaccc ataagtgtac caaataatag tgcatattca
    14641 gattttaaaa agtaaagacc aattccagaa agtactccaa ctataaaaga atctaaaatc
    14701 ttacctgtaa agtatttacc aatatttaaa tttaagcttt gacatagttc tattataaag
    14761 tctccatatt ttttacctaa ggaaatataa actacttttt ttgtaaacag taagaatttt
    14821 tctttttcta ataatatata gaaacaaatt ataaaaccta aaagaaattg tactataaat
    14881 tttccaactg aaaatgtagt gctaaatatc ccaccaagtg aaccaataag caagttacta
    14941 aatttaggca ttacactcat aattttgtca ccatactctt ttaatgtatt agggtcaact
    15001 gattgtaaac tcttgcctaa gtcgaataaa aactgctctg ttttatctat gtatgtagga
    15061 atttgagcaa ctaaatcagc taaactattt actatggatg gaactgtaaa taatataaac
    15121 gaaattagta taactatgag tacaccataa gtaagaagta acgaatatat tctcttaaaa
    15181 tttaatttac tttctaagaa ctttactagt gggttgaata tatatgctaa tacaaatgct
    15241 attataaatg
//
LOCUS       DAGSHT010000074        15108 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGSHT010000074 DAGSHT010000000
VERSION     DAGSHT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.076"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            297..974
                     /locus_tag="MJB72_002034"
     CDS             297..974
                     /locus_tag="MJB72_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263142.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            1046..3061
                     /locus_tag="MJB72_002035"
     CDS             1046..3061
                     /locus_tag="MJB72_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY8263143.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVILCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3111..3791
                     /locus_tag="MJB72_002036"
     CDS             3111..3791
                     /locus_tag="MJB72_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263144.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4307..4753
                     /locus_tag="MJB72_002037"
     CDS             4307..4753
                     /locus_tag="MJB72_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263145.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLVIRTYCKHQVS"
     gene            5133..6050
                     /gene="pstC"
                     /locus_tag="MJB72_002038"
     CDS             5133..6050
                     /gene="pstC"
                     /locus_tag="MJB72_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBY8263146.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            6052..6876
                     /gene="pstA"
                     /locus_tag="MJB72_002039"
     CDS             6052..6876
                     /gene="pstA"
                     /locus_tag="MJB72_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBY8263147.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6895..7659
                     /gene="pstB"
                     /locus_tag="MJB72_002040"
     CDS             6895..7659
                     /gene="pstB"
                     /locus_tag="MJB72_002040"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBY8263148.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7687..8340
                     /gene="phoU"
                     /locus_tag="MJB72_002041"
     CDS             7687..8340
                     /gene="phoU"
                     /locus_tag="MJB72_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBY8263149.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8498..9367
                     /locus_tag="MJB72_002042"
     CDS             8498..9367
                     /locus_tag="MJB72_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBY8263150.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9454..10890
                     /locus_tag="MJB72_002043"
     CDS             9454..10890
                     /locus_tag="MJB72_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBY8263151.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10938..11765
                     /locus_tag="MJB72_002044"
     CDS             10938..11765
                     /locus_tag="MJB72_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY8263152.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    12060..12322
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12398..15064
                     /locus_tag="MJB72_002045"
     CDS             12398..15064
                     /locus_tag="MJB72_002045"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBY8263153.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         5994 a         1582 c         2744 g         4788 t
ORIGIN      
        1 atcaatactc aaactagcca atagacaagt ttctagaaaa ataataaatt tatacgtgaa
       61 aatgacttta agctccattc tccaacaatt ggagacttta attagtattt aataatgaat
      121 cttaatgttt aatacaatat tcaagaatta ttcaaaaata cttaagtatt atagtctagt
      181 ttgtaaagat attataaaat aataatacca gttatatatg aaaataaaac attataattt
      241 gtaataatta taaaacttgg aattataatt aaaatacctt tgaatggagt ggtgtaatgt
      301 ataatttact ggttgtagat gatgatgttg atattttaaa cataaatcaa atttattttt
      361 ctaatatagg ttttaatgta tatacatcag agactgctaa agatgctatg gatatagtag
      421 agagtatagt tttagactgt ataattttgg atatttcatt gccagatata gatggatata
      481 aaatgtgcaa tgatataaaa gaaaaggtta acatacctat aatattttta tctaattatg
      541 agcatgaaga agaaaaagtt cggggatttt tagctggggg ggatgattat ataacaaaac
      601 cttatagttt aaaagaactt gaacttagaa tatatgctcg tatgaggcag tacaaaaata
      661 ttacatcagt tcctaatgtg ttaaagtttt cagcgttagt aataaatgta aattcaagaa
      721 aagtaacttt tgatggagaa agtatagatt taactactat ggaatttgaa atattattgt
      781 ttctagctga gaacaaagaa caagtatttt caaagataga gatatataac caagtttgga
      841 aaatgcctga tattggagat caacatacag tgcaggttca tattgcacag atgagaaaaa
      901 aaattaattc tttaagcagg gagcatcaat acattcaaac cgtatgggga aaaggatata
      961 agtttgtacc ataagtgaga tactgtaatt aataaatagt tcttgtttta tttaatacga
     1021 ctatgaaaga gatgtggttg atgcaatgaa aaagtataat atactaaaag gaactctcat
     1081 tgtattgata gtctctctgt tggcaataac aggattaaat cttctgtata aatatgataa
     1141 taaatataca tataagtcta ttagagcaag taatggaatt ttaactatag ataatgatac
     1201 tttcactaaa ggtaaacttg tctttttgat agatgactgg gagttttata atcataaaat
     1261 atttaaaccg aatgatttta cagagcaaga tataaaacca gaatatgttt ttataggtca
     1321 atatccagat ttttctatga gaaaggggaa tcagtcacca tatgggaatg ctacttatcg
     1381 tatgagaata aaaaatgaag gcaaggataa gctactaagt ttagagcttc ctgagatttt
     1441 ttccgcaagt gaagtatgga taaatggaga aatggtttct aaattaggag atgtaggaac
     1501 agaaaaatat agacccaaaa ttaaaaattc agtagtaagt tttattgcaa agaaaaatac
     1561 agaaattata atcaatgtat cgaatttttc acattattat agtggtttgt attatccgcc
     1621 tgcattagga gaaacaaagg acataagtaa tatgatgttt tatcgattac ttttttatag
     1681 tataatttgc ttcacgactt ttgcaatagc tatattttca ttgtctgtat ggtttctttc
     1741 aaacaaaagt aaaatttact tatattttgg atgtatgtgt gttttctttg ccatccatgt
     1801 atcctatccc tttgtacatt tgttaggact gcctttggta agctttacat atgcggttga
     1861 agatttgtca tattttatgg taattttatg tattatttgt ataaatggaa gtatatccaa
     1921 aatagaagaa aataaagtgt atagattttt aattttacca ttaggaatta caatgagtat
     1981 catatctata gtgataccaa ttattcttct tccatatgat atgtactttg tcaacatata
     2041 tgggaagtta atagacatat ataaatatat tgtatttata tatatattaa tgtcttcgct
     2101 ggtttcaata tataaaaaga catgctctga gcatataagt tcttatattt tagtttcagt
     2161 aaacactgtt tttggaatta gcatattatt tgatgcagtt acgagtaata gatttgagcc
     2221 tatatttaca gggtggcaaa cagaatattg tgggtttgta attgttattt tattttctgt
     2281 tatgatgata aacaggaata gaataataat aaaagagaat gaaaaactta caaggaatct
     2341 tgaacatgaa gttgaagtga gaactaatga acttacaaca ttgttaaatg aacgtaaaag
     2401 attcttggca gatgtagcac atgatttaaa ggcacctgtt tcggctatac aagcatttat
     2461 agatttgata aaggttggta atattcatgt agatgaagag acaagaacct atctttatgc
     2521 tataaatcaa aagtcaaatg aagtacaaaa tagagtacgt agtttgcaag aatttacatc
     2581 acaagataaa agcattggta ttagtgaaaa aatatgtctg aataatttta ttaaagagat
     2641 atatgaaaat aatttgccag atacacaagc atgtggtgta aactttgaaa cttacgtatt
     2701 ggagaaaaaa atatatgcaa agataaataa agagatgctt acaagagcat ttgagaattt
     2761 aatttataat gctttgacct ttactccatt tgatggaaag ataattttat caatggataa
     2821 agaagatgga tttgcaatta ttaaaatttc tgataatgga aaaggtatac ctccagaaaa
     2881 tataccaaag gtatttgaca gattctttac agaaagagac aataatgagc caaaagggca
     2941 agggttaggg ctatatattg tcaaatccat tataagagag catggtggag aaatctttgt
     3001 agattcagaa ctaggaaaag gaactgtttt tacaataaaa ttaagtctat gttttttata
     3061 ataatagctt atttaatata aaagaataat gatatttggg agtgattata atgcaaacta
     3121 agatattgat aatagatgga gacaaggata attgccagaa gctaaaagga tttctggagg
     3181 aaaaaggaat aagtatagat ttagcatata attgtgaaga agctattgga aaaatattta
     3241 gcaataaata tgatttaata tttttagaaa taattctaag tgatggtgat ggatggactc
     3301 tttgtaaaaa aattagaaat gtaactactt gtccaattgt ttatatgact tatataaatg
     3361 aagaccaaag cattttaaat gctttaaatt ctggtggaga cgattactta ataaagcctc
     3421 taaatttaga aatattatat gcaaaagtaa aagctatttt aagaagaatg aactcatatg
     3481 taaataataa tgaaaataat acaaaattag aggaatataa tagaactagt ggagtaataa
     3541 agttagaaga taaaataata aaattgactc caacagaaaa taagttgtta aactttttta
     3601 tagagaatcc tgaaaagact ttgactacta aagagatata tgaacatgta tggatgaatg
     3661 aatatttaga ggataactat agtgtggtag ttgcagtgaa tggattaaga aagaaaatag
     3721 agaaagatta taaaaatcct cagaaaatta taacaataag aggagcagga tattatttta
     3781 ataaagaata ataaatatat taatatcatt aataattgtt ataaatgtga aatactaacg
     3841 atatctaaac taaattatta taaaatagct gactgtaatt taattgataa agcctattct
     3901 tctagacttt gccagttaaa ttacagtcag ttttattata taaaaaaatg cgtaacagaa
     3961 ctattatttt atagattata tttgaaaaga taaataaatt gtatatctaa atttatatat
     4021 tattttatta tttaatatat attaaataat aaaatagtta aataataaga ttaagaattt
     4081 cttaataaat ccagaagatg ttttcgatta tataatattt tatattttta tattattaat
     4141 tatatgtgtt gcaattgctg cacctacttt ttctggtgag tatcagacac aatcagatag
     4201 catccttcgt tgtacgaaac atggacatat tcgtctagcg tttactaaaa tattagctct
     4261 attttctata tttataataa tgtttgttat atgtatttct atccatttgg caatttctga
     4321 tttagctttt ggtactgaat gcttgaaaac ctcatttcaa atgttgtttt ctgtaattag
     4381 tctagtaaat ataaatttac tacaagcaca gattcttata gctattggtg gtctaatttc
     4441 aatacttgca atggttagtt taacattatt tttatctgca aaatgtaagg attctcttac
     4501 ttctatgctg attgcttttt ctatttgctt aattcctatg ggaataggtg ttagttggat
     4561 tggataccta ttaccaccag ggggaattgg ttttaagatt agtttattat atcaaatgac
     4621 atcatttaat ttcttacact taggaggtat gagtttttgg acaccacaca ttatactgtt
     4681 tttctcggtg atagaaatac ctatttttat atttttggtt attcgtacct attgcaaaca
     4741 tcaagtatcg taacaatttg atggaaataa tagtgatata aactagagga tacaataatt
     4801 atatatgtag tataaaagtt aaatttagtt aacaaatctt aatagcagat ataggtaata
     4861 aattatacat aaactgtatt atacctatat ctgctatttt aatatagaga aacaacagta
     4921 tattgtaaag taaatgtcaa atatagtttt aaaagtaaaa ttaatgttaa aaagtaaata
     4981 taattttaca cttgcttaac ttaatcctaa tatagattta acttaaaaaa agtagaataa
     5041 aagtatagga aatttgatga agacgaatta taaaaaatta aattttgaat acagaacgaa
     5101 aatcgataag tatattttaa tgggagggta ttatgatagt aaaatctaga ttagtaggtt
     5161 tagaaaaaaa tgacaataat ggcaataaga caaaatacgt aattgaaaaa gtagcaaaaa
     5221 atatttttct acttagtgct ttagtagcag ttgcaagttt acttttgata ataggattcg
     5281 tattttataa aggattaaga ccatttatat ttgaaggata ttcatttata gactttttaa
     5341 ctggtgcaga ttgggttcct tctgccaata aatttggaat atcatctatg atagtagcat
     5401 caatatttgc aacacttgga gcacttgtta taggagtacc tgttggtata ttaacctctg
     5461 tatttatagc agaagtagca cctaaaagat tagcaaaaat aatgtcacca gcagttgaac
     5521 ttttagcagg tataccttca gtattatatg gtgtatttgg tcttgcagtg atagttccaa
     5581 ctatacaaag tatatttaat cttccaaaag gtcaaagttt gttagcaata gtaatagtat
     5641 tagcagtaat gatgttacca acaataatat ctgtatcaga aacagcaata agagcagtac
     5701 caaaagcata caaagaaggt tcattagcac ttggtgcatc aaaaatagaa acaatattca
     5761 aagttgtatt acctgctgca aaatctggag tattagccgc agtagtgtta ggagtaggaa
     5821 gagctttagg tgaaacaatg gcagtaatac ttgttgcagg caactcacca gttatgccaa
     5881 ctgcaataac agatagtgta agaccactta caactaatat agcacttgaa atgggatacg
     5941 catttggtac acatcaagaa atgctatttg caactggagt agtattgttt acatttatat
     6001 taattttaaa cttagtttta aataaacttt caaataaggc ggtaaattaa gatgagaaag
     6061 ttcaaagaga atctattgaa gtcattagta tatctatcag cactatttac aatagtatca
     6121 ttagtagtta tagtgggatt tatatttata aagggaattg gcaacatgaa ttttaatttc
     6181 ttatttagca attattcagc atcaggtgat ggtggtatac ttccaatgat aattacaaca
     6241 ttatatacag tagtagtttc tatagttgta gcaactccta taggaatatt ggctgctata
     6301 tatttacaag aatatgctaa aaaaggaaaa gcagttacag ctataagatt tgcaactgaa
     6361 agtttatcag ggataccatc tatagtatat ggattatttg gaggaatttt ctttgtagtt
     6421 acattaaaaa tgggatattc catagttgca ggttcactta cagttgcaat aataatatta
     6481 ccagtaataa ttcgtacaac agaagaagct ttaaaaacag ttcctcaagc ttatagagaa
     6541 tcatcattgg cattaggagc tacaaaattt caaactttat ataaggtagt acttccaagt
     6601 gcaataccag gaattttatc tggagtaatc ttatcagttg gtcgtataat tggagaatca
     6661 gctgcaatat tacttacagc aggtactgta gccaagatgc caggtggaat atttgatagt
     6721 gcaagaacat taactgtaca ctcatactta ctgactaaag agagtggaga tatagcaaca
     6781 gcagccagta taggaattgt tttaatagta attgtgttag cactaaacat gctagcaaga
     6841 tttgttgcta agaaattaaa taaagctaat tattaaattt aagaggtgtg aataatggaa
     6901 ttaatcgata agataaaaat gagtgtaaaa gatttggatt tattttatgg tgataagcaa
     6961 gcattaaaga aaataaatat ggatataaaa gagaataagg ttactgcttt aataggtcca
     7021 tctggatgcg gaaaatcaac ttttataaga acgttaaata gaatgaatga cttaatcgaa
     7081 gatgtaacaa ttaaaggaaa catttctgtt gatggagagg acatatatac ctcagatgat
     7141 gttataaatc ttcgtactaa agtaggaatg gtatttcaaa agccaaatcc atttcctatg
     7201 agtatatatg ataatgtggc atatggtcct cgtacacatg gactaagaga taagaagcaa
     7261 ttagataaaa ttgtagaaga aagcttaaaa ggtgcagcta tttgggatga ggttaaagac
     7321 aggcttaaat catctgcact aggactttct ggtggtcaac aacagcgtat atgtatagca
     7381 agagcaattg ctatgagacc agaagttatt cttatggatg aacctacatc tgctttagac
     7441 ccaatttcaa cattaaaagt agaggaactt atagaggatt taaaaaaaga ctacacaata
     7501 gtcatagtta cacataatat gcaacaagct gctcgtatat cagatgaaac agcttttttc
     7561 ttaaatggag aggttataga atttagcgat acaaaaacta tgtttaccac tcctgtagat
     7621 aaaagaacag aagactatat aacaggtaga tttggttaaa atatataact ataaaggagt
     7681 tagacaatgg tcaataatag tttagatata agtattgata ctttaaaaga acaaacagtg
     7741 cttatgatgg aaaaatgtga agaagcaata gaaaagtctg tgacttgtat gatagagaag
     7801 gatttagagg gtgcaagaaa agtagtagaa gatgatgaag aaataaataa attaagagaa
     7861 gaaataagag ataagagtat agagctaata gtattaaagc aaccaatggc aagggactta
     7921 agatttattt attcattagg taatatatct atagaactgg aaagaatagg agattatgca
     7981 gcaaatatag ctagagaaat gttgaagata ggtggagagg cttatataaa agagctaata
     8041 gatattccta agatggcaaa agaatgtaag tctatgatgt ctcagactag agaagcttta
     8101 atcttatttg atgcaagtat agcttatgat gttggaacta aagatgatat tgtagatgga
     8161 ttgtatgaac aagtacaaat tgattgttta aaaataatgc atgaaaactc aaataccata
     8221 gaccaagggg taaaacttct acttatagga agataccttg aaagaattgg agaccatata
     8281 acaaatgtat gtgagaagat aatttttgca attgagggta aaatggttga gataggataa
     8341 aaaactgctt taaagcagtt tttttattgt ctaaattaag aaagaattga taaaaaaata
     8401 tgattaatgg gtaaatttaa aaaattgtga tatactagga acatattaaa tatgaggatg
     8461 cgtattttat ataaggtgct ttttaggagg tggaactttg aaacttcaaa agatgggtat
     8521 gaggactata aaaacaggaa ttagtgtcac tttatgtatg attcttggtg actatttagt
     8581 agagaatatg ttttattctc tggtagcatg tataatttct gttcaagata cagtaaaagg
     8641 ttctttaaag acgggaatca atagagtgga aggtactatt ttaggtggta taataggatt
     8701 catatttgcc atcattaaag ctggagaccc atttttatgt ggtctaggag taatgtgctc
     8761 aatatattta tgcaatctgt ttaaaataaa gtcagtagta gttgcctgtg taacctttct
     8821 ggctatacat ctaggtgtag gaaatagtga ccctgtatat tattctttac atagggtgtt
     8881 agatacatct gtgggtgttt tgtttggagt acttgttaat tattatgttg ctagaccaaa
     8941 ttatctaaat agtgttatag atgagtttaa aaagatagaa gaactttcaa tgttgcttat
     9001 tgaagataaa attcttagaa aagaaaattt aaatgtagag aaatttgaag aagaagtaga
     9061 aaaattagaa gaaatatatt caaagcttat tgatgagttg gattatagtt tgagtgaaac
     9121 taatatagaa aaaattgaga atgccttaag gatatgtaga gaaatacatt ttcatatgca
     9181 gtctatagag ttacttgaaa ataaacttta tttgaaagag catacatata aataccttaa
     9241 gaaaacatat aatgtggata agctagactg gaatatagat gaagaaaaaa gccctgtatt
     9301 caactatcat ctaatgaaaa taatgattga aatagagttg ttgagaaagc taaataaatc
     9361 atattaataa tagtagttaa ataataaata aaaaatagta tctttgtaaa tattaaacat
     9421 atataagttt aatttatgga ggtactattt tttatgaata ctagaaatga caattctaaa
     9481 atactagaaa tatataaaat gaagcttttt ggcgaacttg taaaagcagt gtggaatgat
     9541 gatatagagg atattgaaaa cttacctaat aaaattttaa atgaagattt aaaggataat
     9601 ttaaataatt attttagtgg aaaatttgat aaagatacta tggtaaattt aattagggta
     9661 actatgggat tggatgtgac agaagataca gataaaaatt taaaagagtc actacatgaa
     9721 gctgtaaatt tagatgaagt caaaagacca ataatatctg taatatcaga tgcttgcaaa
     9781 tattgtgatg aaaataataa ggaagatgag tcatgtaaga taagaagtaa gcatattaat
     9841 tgtaatgagg aagatgtatg tagtgcttgt ggtgattgtg tatcaagatg taagttgggt
     9901 gcaatttcag ataaaataga atttatacca atgataaaat tgttaaaaga tataaagagc
     9961 cctgtatatg caatagttgc accagctttt gtaggacagt ttgggaaaga tgtaagccct
    10021 ggtaaattaa gaactgcatt aaaaaagata ggatttgaag atatgataga agttgctttg
    10081 gcagcagata tgcttactgc aaaagaagca tatgattatt atgagcatat gaaaaaggac
    10141 gaagaaggat gctttataac tagttgttgc tgtcctattt gggttagttt gatacaaaat
    10201 agttttcctc agatattgga aaatgtatca ccatccgttt ctcctatgat tgcttgtggg
    10261 agagctgtaa aaattttaaa tccagatgct aaagttgtat tcataggtcc ttgtatggca
    10321 aaaaagaaag aagcaatgtt agaagacata aaagatgcag ttgattttgt gcttacattt
    10381 aaagagttgc aagaaatatt tgatgcatta gagttaaatc cagcaataat ggatgagagt
    10441 aatagagtag aggcgtccct ttcaggtaga gtttatgcta gaacaggagg tgtaagtaaa
    10501 gcagttaagt tatcagttag aagaattgac aaaaatataa aatttgaaga aaaggcattt
    10561 gaaggaacaa aagagtgcat ggacggtcta aagaaagttc taaataaaga aatagaagct
    10621 acatttatag aaggaatggg gtgtgtagga ggctgtgtag gaggtccgaa gcgtatttta
    10681 agtgtggaag aaggtactaa gaatgttgat gaatatagtg aaagtacgaa tatggaaact
    10741 ccatttgata atttaaatgt attacaattt ttaacttctt tgggaatcaa aagagtagaa
    10801 tctttaggag aaaaagaaga agaaaaggtt aaaaaaatat ttagtagaga tataaaaagt
    10861 aataagaaag agaataaata taaaaaataa ctttttaagt cttaaactta tgcttttata
    10921 aaagggagat atgggggatg aaaattaaag ataataaggc tgtttttcat gtatttataa
    10981 ggatatttat tatagctgtt ttaattttaa ctggaacatt attggaggaa aaaattaata
    11041 atgttccagt tgaaaatata agttcaaaag gtgaaaatac actggaactg ctttctcaaa
    11101 gtaatacttt aggagaaatc atatacaaag catctcaaga agaaacaaaa ggtccagggg
    11161 ctacttctga aaaagtagca tacataacaa tagatgatgg tccatcaaaa tatacaaatg
    11221 cactacttga tattttaaaa gaaaatgatg tcaaagcgac atttttcatg cttaatcaga
    11281 atatgaaaaa ccacaaggaa gaagtaagga gagtcttgga agaagaaaac tcaataggtt
    11341 ttcatagtgt aagtcatgat atacataagt tatataaaag cccagaagta acagttgggg
    11401 aatttaatac atgcaataat actctacaag aaataactgg tcagagttca aaattaataa
    11461 gattaccata cggaagtaaa ccatatatgc cagaaggttc atataataaa cttatggcaa
    11521 atgattattt aatttgggac tggaatttgg atacacagga ttggaagtct tctacaagtc
    11581 agatagtaag taatatatta tattatggaa gaaaaagaga tgaattagta gtattaattc
    11641 atgagaaaga gcaaagttta aatgctttaa ataatattat aagaatctta aaagagagag
    11701 gatatacaat attgccaatt acagaaaata taacacctaa aaatttctgg caagaaaact
    11761 tataataaat ttgaatatag taaaagataa agtaaattat ttataaaata aaacatataa
    11821 tataaaaaat acgaataaga ggtatactgt aaggataata taatataaaa ttaatgagaa
    11881 agcattaaaa taaaataatc ttgcaattat tgacattata agacaaaata actgtaaaaa
    11941 catattttaa aatataacaa ctatataaaa gagaataaac ataattaaga aaattcagaa
    12001 aaaataaaat aaaaataatt gacaaagaga ataatatttg tatataataa tatgtaatat
    12061 taaagactga gaagaagagg agtaagtaaa tactactaaa agagaggaaa attcactggc
    12121 tgtaagattt tctaagtata agatttactg aatgtagctt tggagtttct aaactgaact
    12181 ataagtaagt ttagacggtt agaaccgtta aattctttga gagtcaaagt tgataaaata
    12241 acattaacca ataatttaat tatatgttaa tgtattttaa gctttggaat taaggtggta
    12301 acgcgagctt ttcgtccttt ttaaagagga tgaagagctc ttttttattt ctaaagatat
    12361 ttaaaaaatt tatttataaa atacaggagg caggacaatg gaaaatacga atttatcaaa
    12421 aacttataac ccaaaagatt ttgaggctag gctatataaa aaatggatgg atgaagggta
    12481 ttttaaatct aaaccaaatc cagataaaaa accatttaca ataatgatgc caccaccaaa
    12541 tataacaggg caattgcata tgggacacgc tcttgaccat acacttcaag atatattaat
    12601 cagatggaaa cgtatggatg gatatgaagc tttttggctt ccaggaactg accatgcttc
    12661 tatagcaaca gaagttaaag ttgttgaaag aataaaaaaa caagaaggca agacaaagta
    12721 tgaaataggt agagaagaat ttttaaagag agcctgggaa tggaaagatg aatttggagg
    12781 aaaaatatct aatcagctta aacaactagg tgattcttgt gattgggata aagaaagatt
    12841 tactatggat gaagggtgta atgaagcagt tatagaattt tttgtaagcc tatatgaaaa
    12901 aggtcacata tacagaggaa atagaataat aaactggtgc ccagattgca aaactacttt
    12961 atcagatgca gaagttgaac atgaagaaca cgacggaaac ttttatcata taaagtaccc
    13021 actaaaagat agtgaagact ttttagaaat agctacaact agacctgaga caatgatagg
    13081 agatactggt atagcagtaa atccagaaga tgatagatat aaacatttaa taggaaaaac
    13141 agcaatactt ccattagtag gaagagaact tccaatagtt gcagatagtt atgttgattt
    13201 agagtttgga actggtgcag ttaagatgac tccagcacat gaccctaatg attttgaagt
    13261 aggattaaga cataatcttg agcagttaaa tactatgaat gaagatggaa ctatgaatga
    13321 agtttgtgga aaatatgaag gtatggatag atttgagtgt agaaaagcta tcgtagcaga
    13381 cttaaaagaa caaggttacc taattaagat aaaagagcat aatcataatg taggaacttg
    13441 ttatagatgc catacagtag ttgagcctag attatcagag caatggtttg ttaagatgga
    13501 agaacttgca aaaccagcta tagatattct taaaaaagga gaattagaat ttgttccaga
    13561 taaatttgat aaaacttatt tacaatggct agaaaatata agagattggt gtatatcaag
    13621 acaattatgg tggggtcatc aaataccagc ttattattgt caagagtgtg gagaaatagt
    13681 agttgctaga aaaatgccag ataagtgtcc taaatgtgga agcactcatt ttaaacaaga
    13741 tgaagatgcc ttagatactt ggttcagttc tgcattatgg cctttctcta ctctaggatg
    13801 gccaaataag actgaagctt tagattatta ttatccaaca agtgtacttg ttacaggata
    13861 cgatataata ttcttctggg tagttagaat ggcatttgca ggtatgttct gtatgaatga
    13921 aaagccattt gaccatgttt tggttcatgg attagtaaga gattctcaag gtagaaaaat
    13981 gagtaaatct ttaggaaatg gtatcgaccc attggagata atcgagcaat atggagctga
    14041 tgccctaaga tttacattaa caactggaaa ttccccagga aatgatatga gattctatat
    14101 ggaaagagta gaatttgcta gaaactttgc aaataagttg tggaatgcat caagatttgt
    14161 atttatgaat attgatgaag atataattaa aaacatgact agagaaagtg ttaaagagga
    14221 cttaactcta gctgataaat ggataatatc aagagcaaat aatattgtaa aagaagcaac
    14281 taataacatg gataagtttg atttaggaat agcacttcaa aagatatacg attttacttg
    14341 gtcagaatac tgtgactggt atattgaaat ggttaaacct agattatatg gagaagatgc
    14401 aaatgctaaa tctgcagcat tatatacatt aacttatgtt cttgaaaaaa tattaaaatt
    14461 attacatcca tacatgccat ttataacaga ggaaatatat actcatcttc ctactgttga
    14521 aggatgtata atagtatctg aatggccaaa atataatgaa gaagataaca tggctgaaga
    14581 agaagatatg atgaacttat tgatggaagg tataagaagt ataagaaatg tgagagcaga
    14641 aatgaatgta ccaccttcta agaaagctaa attgataata atcccaagtg aagaaaaaat
    14701 agaagctatt gagcttggta aagactattt tataacttta gcatctgctt caaatgtaga
    14761 gatagcaaaa gataaatcta atgttccaga agatgcagtt ggagtagtta ttgatggtgt
    14821 tgaaatattt atacctctta atgaattagt tgattttgaa aaagaaattg agagattatc
    14881 taaagaaaag aaaaagttag aaggcgaaat aaagagagtt aatggaaagc ttgcaaatca
    14941 aggtttctta gcaaaagctc ctgaaagttt aattgaagaa gaaaaagcaa aaaaagaaaa
    15001 atttgaagag atgattaaat ctgttgagga aagattaaca aatttagaat ctaaaataaa
    15061 ataaatataa gaattagtaa aaacatacaa aaagtatata ttctatac
//
LOCUS       DAGSHT010000075        14805 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGSHT010000075 DAGSHT010000000
VERSION     DAGSHT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.077"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(205..1563)
                     /gene="rlmD"
                     /locus_tag="MJB72_002046"
     CDS             complement(205..1563)
                     /gene="rlmD"
                     /locus_tag="MJB72_002046"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBY8263154.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(1738..3498)
                     /gene="pyk"
                     /locus_tag="MJB72_002047"
     CDS             complement(1738..3498)
                     /gene="pyk"
                     /locus_tag="MJB72_002047"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBY8263155.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(3550..4509)
                     /gene="pfkA"
                     /locus_tag="MJB72_002048"
     CDS             complement(3550..4509)
                     /gene="pfkA"
                     /locus_tag="MJB72_002048"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBY8263156.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(4718..8287)
                     /locus_tag="MJB72_002049"
     CDS             complement(4718..8287)
                     /locus_tag="MJB72_002049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBY8263157.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(8444..9391)
                     /gene="whiA"
                     /locus_tag="MJB72_002050"
     CDS             complement(8444..9391)
                     /gene="whiA"
                     /locus_tag="MJB72_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBY8263158.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(9408..9833)
                     /locus_tag="MJB72_002051"
     CDS             complement(9408..9833)
                     /locus_tag="MJB72_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY8263159.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(9902..11008)
                     /locus_tag="MJB72_002052"
     CDS             complement(9902..11008)
                     /locus_tag="MJB72_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBY8263160.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(11020..11877)
                     /gene="rapZ"
                     /locus_tag="MJB72_002053"
     CDS             complement(11020..11877)
                     /gene="rapZ"
                     /locus_tag="MJB72_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBY8263161.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(11908..12621)
                     /locus_tag="MJB72_002054"
     CDS             complement(11908..12621)
                     /locus_tag="MJB72_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBY8263162.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(12625..13539)
                     /gene="murB"
                     /locus_tag="MJB72_002055"
     CDS             complement(12625..13539)
                     /gene="murB"
                     /locus_tag="MJB72_002055"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBY8263163.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(13812..14582)
                     /locus_tag="MJB72_002056"
     CDS             complement(13812..14582)
                     /locus_tag="MJB72_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBY8263164.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         4420 a         2922 c         1582 g         5881 t
ORIGIN      
        1 aaaaataagt tgtataaaat ctggctactg tagattgacg atttctttta aaattacaat
       61 tataataagt tttatagaat taaaaataaa actttagaga tttttgataa cattgtagac
      121 taaatttgcc accaaaatcg ctttacagtg gcaaatcggt ggcaaataca atatattatt
      181 agtatataat ctatataaac attactattg tttgattatc ttagctacag tttccacatg
      241 tgtagaatga gggaacatat ctacaggttg tgcctcaaca catttaaatc cgtgaacatt
      301 tagataatct aaatcccttg ctaaagtaga aggattacaa gaaacataga caattctttc
      361 cggattcatt gaaacaatag tctctaatac cttttcgtca catccctttc taggtgggtc
      421 tacaactact acattggcag tcttaccctc tctatacatc ttaggtacaa tttcctctgc
      481 ctttccaaca taaaattcag cattatgtat attgtttaat ttagcattta ttttagcatc
      541 ttttatggca tcatctataa tttctattcc ataaaccttt ttagcctttt gtgccaaaaa
      601 taaagatatt gtacctatcc cacagtaaat atcaaaaaca gtatcagtat catttaaatt
      661 tgcatattca agagccttat catacaatac tttagtttgg acagggttta cttggaaaaa
      721 tgataatggt gaaatctcaa acactaagtc acctatataa tcgtttattt ttccatctcc
      781 atatataact atattttctc ttcctagaat agcattcgta tctttttgat ttacatttac
      841 aaccaaagtc ttaaatccag gtatattttc cttaagcact gaagctaatt ctttaagata
      901 tggaagtttg tttccatttg ctactaatac aaccattact tctttagttg taaaacctat
      961 ttttgttact aaatgcctaa tcaaccccgt gtgagtcttt tcatcatata tacttacctt
     1021 ataggccctt atatatgttt ttattatttt aacaatttta tcatttatct catgttgtat
     1081 tacgcatttt tctgtcgata ttacatcatg agtcttcttt ttatagaagc caataattgg
     1141 aataccatta ttcttttgta ttggaaattg tgctttattt ctatatctat atggattatc
     1201 cattcctata gtgtcatgaa cttctacatc ctctaactta cctattctcg aaagtacttg
     1261 tttaacttca tttgttttca tttcaagttg tttattataa tctaagtctt gtatctgaca
     1321 ccctccacaa tccctaagat tgtcactaca cactcttttc accctgaatg gagacttttc
     1381 tattatctca acaatatctc ccacagcata atttttcttt actttattaa tcctaacttt
     1441 aactttatca tgtattaatc cgccctctac aaaaactgta ataccattgt actttcctac
     1501 tcctactccg ccttgaccaa tgtctactat atctacaata tacatctcgt ttttcgaaag
     1561 cataaatcac cctccaatca atctattata tttaaatctt tacaaacttt tttctttaaa
     1621 tttatattaa aatattgcta ttaattaaac tatatttttt actaagctac aatattttat
     1681 tttaccaaaa aaaaaggagc ttataaagcc ccttagtaaa taaatttatt atttttctta
     1741 taaaactcta gttgaacctc tatataccac gcctcttgat gcatccacag ttacaacttc
     1801 tccatctttt actagatttg ttatatcagc tgcagacaca ataactggta tatctaaatt
     1861 tatacctaca atagctgtat gtgatgtcat tccaccattc tcagttacta cagctcctgc
     1921 tttttctata taattgttca tttcagcatc agtagcagta gaaacaagta tgtcgccttc
     1981 attaaagtct acacaagcat ttccattctt tattatacga actttacctt ctaccgtttg
     2041 agtacctaca cctatacctt gcacgatttc ttcgcttatt acatgaacct ttattaaatt
     2101 agtagttcca cttactccta ctggtactcc agcagttata actactaatt ccccattatc
     2161 tatataatca gcttgtctag ctgcctcaat tgccttttct ataacttcat ctgtatttcc
     2221 tgcaacagaa gacttaactg gatatactcc ccatgtaaga gctaatttat tcatagtctt
     2281 ttcattattt gtagtagcta ttattggaga ttttggtcta aatttagata ccattctagc
     2341 agtatatcct gaagaagtag atgttattat agctgatgca tttaaatcta ctgctgttgt
     2401 acaagttgca tggcttattg catctgttac agtaacattg ttagtaccat tttcctttag
     2461 taatctatca taatctaaag tctcttctgt tctttttgct attgtagcca tcatttttac
     2521 tgcctctaca ggatattttc cagcagcagt ttctccagat aacattatcg catctgttcc
     2581 atcatatata gcattggcaa cgtctgtaac ttctgctctt gttggtcttg gatttcttat
     2641 catagaatca agcatttgag tggcagtaac aactggcttt gctaattcat tacatttttt
     2701 tatcatcatt ttttgaacta ttggcatctc ttcagttggt atttcaacac ctaagtcacc
     2761 tctagccacc attataccat cagaaacttt taatatttca tctaggttct ctacaccttc
     2821 ttggttttct attttagata ttatttgtat atcagttgca ttattgttct ctaatacttc
     2881 tcttattgct agtacatcag atgcctttct tacaaaagaa gcagctatat aatctattcc
     2941 ttgacttatt ccaaactcta tatcacttat atctttagga gttattgctg gtaaatttat
     3001 cttaacacca ggtacattta ctcctttatg atttttaact attcctgaat tttcaacaac
     3061 acaaactata tcttctccat ttatttcttt tactcttaag ccaactaaac catcatctat
     3121 tagtatagaa tctcctgatt taacgtcttc agcaagacct ttatagctta ctgtacacat
     3181 ttctttagtc cccataatat ctttcatagt tatagtgaac ttttgccctt cttctaaaaa
     3241 tacctctggg tcttcaaagt tacctgttct aatttctgga cctttagtat ctaataatat
     3301 agcaactggc ttatttaatt tttctctaac tttctttgcc atatcaattc tttctttatg
     3361 ttcttcatgc gaaccgtgag aaaagttaaa tctacacaca tttagaccat tttgcataag
     3421 ttgagttaaa acttcctcac tttgtgatgc tggacctagt gtacaaacaa tttttgtctt
     3481 ctttacatta tttaacatat ttgctcctcc tgagatatta ttccacagat ttatttttaa
     3541 tactaaaact tatatagata atatttttgc catctcataa gcttctttat caaatacttt
     3601 cttttgagct aaagcttcac ttatttctaa atcaattatt ttgttttctt ttattcctac
     3661 aactctagct gattttccat ctaataataa ttctactgct cttactccaa gtctacttgc
     3721 tagtattcta tcagatacag ttgggcttcc tcctctttgg acatgcccca atacagtgac
     3781 tcttaaatct gctcctgact cttttttaag ttccttttct aaatcactag cacttccgac
     3841 tccttctgct aaaacgatta tactatgtct cttacctctt ttttgagtag tctttaatct
     3901 tagagcaact tcatctactg taatttctac ttctggtact attatagttt ccgctcctcc
     3961 tgcaagacct gcatataagg ctaaatctcc acagtgtctt cccataactt ctactatgtt
     4021 aactctttca tgagatgatg atgtatctct tatcttacct atagcatcta taatagtatt
     4081 cattgcagta tcaaatccta tagtataatc tgtatatgct aaatcgttat ctatagttcc
     4141 tggtatgcct atagctggga atcctaagtc actaagtttt tgggctcctg caaatgaacc
     4201 atctccacca attacaacta agcaatcaat tttatacttt ttaagaattt ttactgcttt
     4261 taatctacct tcttctgttt taaattcttc acatctggaa gattttaata tagttcctcc
     4321 cctgtgtata atatctccaa cagaagataa gttcatttca gttaaatctt cttctagtaa
     4381 tcccttatat cctctattga ttccatatac tttgcatcca taatatattg ctgatcttac
     4441 aaccgctcta atcgcagcat tcattcctgg agcatctcct ccacttgtta gtaatcctat
     4501 agttttcatt ttttacctcc ttggatttta tgtcaatttt ttattataat cattgttatt
     4561 gtattctaat ggactttttt tgtccctata tcacttttta atcccatcaa gactaaaaaa
     4621 attaaatgtc cttaaatgtc cattttgtga tattatatca caaaacggac aatatttttc
     4681 atattattat aatatattta gatttttata taaaaaatca agcttttact tttatgttat
     4741 ctttaggaag taactcttct aagttattta taaattcatc acatatacaa gtcaatgccc
     4801 cggtcatttt atatgcttta cgtttaccat ttatattttc atccataggg tacaagtata
     4861 tactgtcatt acctggatat tgtttggcta tttctacaat attattagtt gtatcagtat
     4921 cagacatact atctattttc aaatacaatt ttttaccact ttctggctta atattttcta
     4981 catttctagt tcttcctgaa ttgtagtttc tatctgcaaa gtcagtatca tcatttatat
     5041 cttttatctc tctagctata agttttgcat tttcgccttc ttttatactt aatgttcctt
     5101 ttacatatat tatattatct tcattcaaaa tgacattata cttttgtaat aattgaggaa
     5161 aaactattat ttctatagca ccatacaaat cttctaattc aataaatgcc ataatttcat
     5221 ttcgttttgt tgttttaata gttttagtta caatcattcc acccattata acatctgttt
     5281 cattcatttg taggtatgac tcttcttcct cttttaatga atttaatttt ccattatcca
     5341 tagatgtttt ttcttgtagt tctttttcaa attgtgataa aggatgtcca cttacataca
     5401 ttcccaagac ttctttttca aggttaagtc tctctctttc ttcgtactct tgccttttag
     5461 gcaaactgta tatttgttga aaatcactat cttcctcact atctccaaaa gcatcaaata
     5521 aagaaatttg accttgaaca tttttctttc tatccataga tacactttct aataaacttt
     5581 cataaccagc catcagacta gctctatttt cacttatatt atcaaaagct ccacatttta
     5641 ttagactttc tattactctc ttatttgtat ctttttggtc taatcttttg actaaatcta
     5701 ctaaatctac aaataatcca ttagattctc tttcagcgat tatattgttt attatattta
     5761 cgccaacatt ttttacggct gctagaccaa atcttatatt gtcgccttca actgaaaact
     5821 ttgaaaaact tttgtttata tctggaggta atacatctat ttctagtgct ttacattctc
     5881 ttatatactc aactacctta tcagtatttc ccattacact ggttatcaat gctgccataa
     5941 attcaactgg ataatatgtt tttagataag ctgtctcata tgctaaaacc ccatatgcag
     6001 ctgcatgtga tttattaaat gcatatctag cgaagtctat catatcatca aatattttat
     6061 tagctatttc ctcttctaca ccatttctga tacaccctgc aatttctata ttaccatctt
     6121 catcaaattt accatgaata aaatattgtc tttcttcttc catcacgtcc atctttttct
     6181 tactcatggc tcttctaact aggtcacttc gtccgtaact ataaccacct aaatctctaa
     6241 cgacctgcat aacctgctct tgatatacca aacaaccata tgtaacttcc attataggct
     6301 ttaatttttc atgtagatat gttacatctt ttgggttgct tttatttttt atatatgcag
     6361 gtatagaatc cattggacct ggtctaaata gggatattcc cgctacaata tcttcaaaat
     6421 tgtctggttt tagctgtttc ataaaacttc tcatgccagc actctccaat tgaaacaccc
     6481 ctagtgtatt tcctgatgaa agcatttcat atactttagg gtcgtcataa tccatctttg
     6541 aaaagtctat tttttctgtg tagtttttta taactctatt tttttctatt aaatcaagag
     6601 tatctcttat aactgtaaga gttcttagcc caagaaaatc catctttaaa agcccaagtt
     6661 cttcaagtgt tgtcatagta aactgtgttg tgattgcatc ttgatgtttg tacaaaggta
     6721 catattcatc tacgggattt tttgatataa caactcctgc tgcatgagta gaagcatgtc
     6781 ttagcattcc ctctatctgt tttgatatat ttattatttc ttttgtttcc gggtcttggt
     6841 catacaattc tttaagatta ggatttgtat ctagggcctt atctattgtc attcctaaag
     6901 caaatggtat ttccttggct accttgtcaa ctttattgta gcctatatcc aatacccttc
     6961 caacatctct tattgcagct tttgcaccca ttgttccaaa agttattatc tgggctacat
     7021 ggtcatcacc atattttctc tttacataat ctataacttc ttctcttctc tcataacaaa
     7081 aatctatatc tatatctggc atggatatac gttctggatt taaaaaacgc tcaaataata
     7141 gagagtactt tattgggtct atatcagtta ttttaagtgt atatgctact atcgaaccag
     7201 ccgcactacc tctacctggt cctactataa tattattttc tttagaaaaa tttataaaat
     7261 cccaaacaat taaaaaatat tctacataac ccattttttc aattacatct aattcataat
     7321 ttaatctctc tagtatctca ttgctaggat tatcatatct ctcttttaat ccattaaaac
     7381 acaactccct taaataagtt tctggtgtat atccttctgg aacatcatac ttaggaagat
     7441 gtatagtgtt aaaatcaaac tccacattac acatatcagc tattttcaca gtattatcaa
     7501 tagcctctaa tgcatatgga aataattctt ccatttcttc tcttgatttt aaataaaact
     7561 catcactacc aaatctcatt ctatttgggt catttaatgt ttttcccatt tgaatacaca
     7621 taagtacatc atgtattttc gaatcttctt tatttacata gtgaacatca ttagtagcaa
     7681 ctagtggtat accagtctct tttgataatt ttactaaacc agagtttact tctttttgct
     7741 caggtaaatt gtgatcttga atctccaaaa agaaattttc ttctccaaat atgtctctat
     7801 agtcaagagc aaattgtttt gctttttcat aatttctatt cattaaagca cgtgctacat
     7861 ctcctgcaag gcatgcagaa agtgctataa tccctttgtt atgcttttta agttcttcca
     7921 tgtcaactct aggcttatag taaaaacctt ctacgtatga agttgaaact atttttatta
     7981 aatttttata tccttccata ttcttagcta acagaactaa atgtccttga tatttgtcat
     8041 agtttgggtc tttatctctc aatccacgtg cagctgtata gacttcgcac cctataattg
     8101 gctttattcc ttcttttata gctgttttgt agaaatctat aacaccaaac atacaaccat
     8161 ggtcagttat agctattgag ctcatgttta gttccttagc cctttttata agttttttca
     8221 ccctcgaaaa cccatcaagt aaactatact cagtatgtac atgaagatga gcaaaatctt
     8281 tgttcattaa atcacctcct gctttataat tttaatttta ccacaaatat atgtactaaa
     8341 acaaaaacag agacttttat ctcaaaatag gaattaactt caacgtatac cattatatgt
     8401 tataaattaa tgcttattct gagattgtcc cctcaattta ttgctatgat ttttttttca
     8461 aatctgttgc aatttcttct atttttctaa gtctatgatt gactccagat tttcctattg
     8521 gtggttctaa catctctcca agttctttga gagtcatatc ctcatactcg attctaatct
     8581 tagctatctc ttgtaaattt tctggcaaac tgctaattcc tatagtctct tgtatgagct
     8641 taatattttc tacttgtctt actgctgcat ttactgtttt agaaaggtta gctgtctcac
     8701 aatttactat cctgtttaca ttatttctca tttcttttac tattttagta ttttgaagac
     8761 ttaaaagagc gtggtgtcct cctataatac tcaacaaatc tgatatttgt tcactctctt
     8821 tgatataaac aacgtgatta ttttttctag ccacaatttt actattaaaa cctaaagagt
     8881 ttataagttc ttttagagaa tctgcaaact cctcattatt agttacaaat tccatatgat
     8941 agttcttttc tgggtcactt atagaacctc caccaagaaa agctcctctt atgaaagctc
     9001 tcttacatcc ttcatgctta attaagttct ctgaaatttt gtttatagta aaaaatcctt
     9061 ctttatcttc aagtatacct aactttttta ataacatttc tgaacccatt tcgcttgtta
     9121 ccattaatac ataactgtta tttctcttta gcatttgatt cttatttact gagattgtag
     9181 tattgatatt aaaattcttt tttaataatt taaatacttt acgggctatt gaatttaact
     9241 ctgttgttat ctttagattt agcttcttat atccaacaat ttgtatactt ccccctgatt
     9301 taactagagc tgacaattca gctatattac aacattcatt cccagatata actcttgcta
     9361 gttcattttt ggtttcagtt gaaaaagaca tatctatcac ctattaacta atttttcttt
     9421 aacctttttt ttatttcttg taaggcaacc tttattatct ccttttcatc atcataccta
     9481 gctaagtcta ctactctatc taggtggata aatttagcat caataaagcc ttcctctttt
     9541 tgtggaatag tatccatatt ctttgcttgc atcaaatacc aaaaaactgt tttatgaact
     9601 gctctatttt catcccagtt ttctttgaat gtatagtgta tttcacctaa atatttcaat
     9661 atatctgctt ttactccagt ttcttcactt acttcacgta atgctgcctc ttggttattt
     9721 tccccttctt ctacttttcc cttgggtaac acccaatctc cattgaactt ccttagtaaa
     9781 agaatcgtat tgccaaacag gactacacca ccagcactaa cctcttctct catcgatgtt
     9841 cactccttaa aactaacata atgataagga tttctacaca attttttaaa atcctttttt
     9901 actaactttt gttgtaatta tgatttaggg ctaactcaat aactatgtta gatatatact
     9961 ttgcatcatg tcttatataa ttatttttta tttcaattaa attcttctct acagttttta
    10021 tacccatctc ttttaattta tctttttgct ttttatctaa caagacttgc tctgctccat
    10081 cttgtttata taagtcaagc atttcttctg gcaatatctc attatttgcg attacataat
    10141 ctattaaatt ctcatcagtg tgttttacta tagcattaac atgctctaat acattatacc
    10201 cttctgtctc acctggttga gtcattatat ttgctatata tacttttgga gctacagacg
    10261 atttaatagc gtctacaatc ccctctacaa gtaaattggg tattatactt gtatataaac
    10321 ttccaggacc cataattata atgtcagcat cataaataga tgcaataaca tcctttaatg
    10381 gttttacatc ttttggattt aaaaaaattt tatcaataga gcttttttga atttttgact
    10441 cttcaggtat actagattct ccatttatta tattgccatt ttctaatttg gcaactaaat
    10501 ttacatcttc caaagttact ggtaaaactc ttcctgttat agcaaaaatt tcacttaatt
    10561 tatatacagc tttttcaaag tttccataca acccattcat agcagccaaa aacaaatttc
    10621 caaaactctg accttttaaa agcccttctg taaacctata ttgcataact tcattcattg
    10681 ttggctctat atttgcaagt gctaataaac agtttcttat gtctcctggt gggagcattc
    10741 ctaagtcttc tctcaataca cctgaacctc caccatcatc agcaacagtt acaattgcag
    10801 ttatattttg cgtagtgtgt ttaagacctc ttaaaaatac cgattgacca gtacctccac
    10861 ctatcacaac cacatttggt tctctatttt caacaataga cctttgaaat agttttactt
    10921 tttcattttt tgctttttta tatataaata acgatacaat taatgcaatc caacctaaaa
    10981 ttcctacaat aataagtaat ttagccatgt ttgcctccat tatcgcaaca tagagtctct
    11041 atgtttcttt acaactctat atcccttatt agataaatcc tccgctataa gatttgttat
    11101 agtaactgac ctatgtcttc cacctgtgca tccaacacca attactagat gttgttttcc
    11161 ttcctctata tattgaggta ctaaaaaatg aatcatatct aaaagtttca cataaaattc
    11221 ttcactgatt ttagaattca taacataatc tctaacttct ttatcatcac cagttttagc
    11281 tcttaactct tcaacataat agggatttgg aagaaatctt acatcaaata ctaaatctgc
    11341 atcagcaagt ataccatgtt taaatccaaa agatactaca gaaatagtta ggtttggatt
    11401 atcttcacca gaagaatata ttttctttat ttcttcctta aggtctttag gtttcatgtt
    11461 agttgtatca attatacatg tagataactc ctttaaaggt tccattatct ttctttccat
    11521 ttttatacct tctggaatct gcatatcttt tgccaatgga tgatttcttc ttgtcatttt
    11581 atatctcttt aacaatacat catcattaca atccaaatat accatttcat attcataatt
    11641 ttctttttct aaataattta agctttcatg aagcgcttca aaaaactttc tccctcttat
    11701 atctatccct agagcaactt tatcaatact tgaatttggt tgataacata attctgcaaa
    11761 tttagttatc aaagcaggag gtagattatc tacacaataa aatcccatat cctctaaagc
    11821 tctcattgtc tcactttttc cagatcctga aagacctgta actataacaa atttcattgc
    11881 taactcctcc cttttatctt atgtttatta tattataatg ttttctacta acgatatatc
    11941 cagattagag tcagatatag tcttcatagc tagttcaaaa tcaccaactc tttctggaca
    12001 atgtgcatct gagcctatta taaatttatt ttctagccct tttattttat ttaattgatt
    12061 tacattcaaa aaagaatggc tactatttat ctcaagtcta gtatttgtct tttttgctgt
    12121 ctttgctact tcttctatat acacatcgcc tttatctcct ggatgagtaa ttataaaaat
    12181 atcattatta cttatcgcat ttattacagc ttttgtatta tattcttttg ctttttcagt
    12241 tttaaaaata tagttattac aatgatttaa taagcttctt aatgatgttg gagtagaacc
    12301 aaaatgatat ccagccataa tataatcaaa atctctaata aatttatcat ccatatctat
    12361 atttccttta tcatctaata tgttacactc catacctaaa agtatattta tatcagtgta
    12421 cttatcattt aaataatcta cttcttctct cattttgaga atatcttttt tctttacacc
    12481 atatgcaata tgtttataac tatggtcaga tattcctatt gtttttattc ctttagaaat
    12541 ggctaccttt acattatcct caatagtccc tttgccatga ctatataatg tatgtgtatg
    12601 ataatcagcc agtattttca tttgttaatc ctctccaagt attttgactt cttcttccaa
    12661 cataacccca aacttggcat atacagcact ttttataaca tatattaaat ccaaaatatc
    12721 tttagcactt gcttctcctt gattgactac aaatccacaa tgcttctcag atacttgtgc
    12781 tcctcttaaa gtaagacctc tcaatccagt ttcttctatt aatttacctg caaaatatcc
    12841 tgttggtctc ttaaatgtac ttcctgcact agcaaaattt aatggttgtt tagttattct
    12901 tctcgtcgct aaatcatcca ttaaactttt tatttcatca taatttcctt cttgtaattc
    12961 taattctgct gataaagcta tatatccgtt tttcgatatt atactttttc tatacccaaa
    13021 ttccatctgt tcatttgata attcaaatat attgccttcc atatccatca gtcttactga
    13081 cttcactatg tccttcattt caccaccata tgctccagca ttcatggcta atgctcctcc
    13141 caatgtgcca ggtattcctg atgcaaactc aaaccctttt aattcttgct tttggagagc
    13201 tttccctaaa actgataata aagctcctga ctgagcagtc atcctagtat catttatctc
    13261 ataagaattg aaattatcag ctagttctat taccactcct cttattccac catctttaat
    13321 taaaatgttt gaaccatttc caattattaa atacggaata gattcttctt ttaccaactt
    13381 aagtacattt tttaattgtt cttcagttct aggtctaact aatatatctg ctggtcctcc
    13441 taccctaaat gaaatatgtt ttttcatagg ttcatcgact tttatatcct ctttacttaa
    13501 tatatttaat aaatttatat atatatcttg attattcatt gtatttcctc ccaaattata
    13561 ctgaacttta gctgtcatta ttatttaaat tacattagta tatagtatta actatattca
    13621 ataatatatg attttgctta tcatatggat tttatatttt aagcttctta attacaaact
    13681 atattcaata aatatataat aaagtaaaaa aaaaaaaata acaacatata aaagaatata
    13741 aataatttaa aaaaagcatc aatatcactg atatcaatgc tttaaaaggc aatttaattc
    13801 gtattattta tctaactatt tttgacatag caaagataat aaatccctcc aactactatg
    13861 gaacctccaa cgatatttcc aagtgttaca aataccaaat tagttataaa ttgaaccata
    13921 gttatatttg ctcctaatgc cattcccatt ggtataaaaa acatgtttgc aacactatgt
    13981 tggaatccac ataatacaaa tagcattatt actgtccaac atgaagccaa ttttgacatt
    14041 atatcttgag atgctgtagc catccataca gctaaaacga ctagaatatt acaaagtatt
    14101 gcttttaaaa acattgatgc tatagttgaa ttaaccttag cttctgccac agctatagct
    14161 tttgtagctg catctcctgt caatgtagaa gaataaaaca caagtgcaac tagcaaaatt
    14221 gaacctacta aatttgcaaa ataaaccagt ccccagtttc ttaacatttt tcctaatgtt
    14281 atttttttgt ccataagtgc aatagccatt aaggtgtttc cagtgaataa ttctgctcct
    14341 gccactacta caagcataag acctactgga aagaccatag cacttaaaaa gctagctacc
    14401 cctgggtcta tcttgccaaa agtttgacta actatcatac tccccaatgc cccaaaacct
    14461 ataaatgctc ctccaaataa acctaataga attgcgtttt gtgtcgaaat atttgctttt
    14521 ttaatgcctg cacttatagt tgcttgtgca gtttccccag gtgtcaagaa tcctttactc
    14581 ataaatcctt cccccttaaa aattatactt tcccttgtct ttttatggca ataattatat
    14641 aataacaata tgatatataa taaattttat taacataaaa aatatactat atacttatat
    14701 atacttgtct catataatta tactatttat tatataacaa ctttgttaat ttcgaaataa
    14761 tttatattga atttgaaaaa tttacctttt gattatttat tttac
//
LOCUS       DAGSHT010000076        14794 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGSHT010000076 DAGSHT010000000
VERSION     DAGSHT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.078"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     repeat_region   538..1095
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=538..566
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     repeat_region   1698..2452
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1832..1860
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            2582..3097
                     /locus_tag="MJB72_002057"
     CDS             2582..3097
                     /locus_tag="MJB72_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY8263165.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREDE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     gene            3162..5555
                     /locus_tag="MJB72_002058"
                     /pseudo
     CDS             3162..5555
                     /locus_tag="MJB72_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            5571..6266
                     /locus_tag="MJB72_002059"
     CDS             5571..6266
                     /locus_tag="MJB72_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBY8263166.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVPAPPKKTNETGRPSVKIE
                     PKRVDSVNAPSNKTYTVKSGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKKVP
                     KYTIYKGQVLRLV"
     gene            6259..8151
                     /locus_tag="MJB72_002060"
     CDS             6259..8151
                     /locus_tag="MJB72_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY8263167.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRFFKNK
                     DTYVLENHTASELVKMLAKDFKLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRLTLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQMFETVDKNMNEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            8165..8425
                     /locus_tag="MJB72_002061"
     CDS             8165..8425
                     /locus_tag="MJB72_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBY8263168.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            8430..8849
                     /locus_tag="MJB72_002062"
     CDS             8430..8849
                     /locus_tag="MJB72_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY8263169.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            8850..9896
                     /locus_tag="MJB72_002063"
     CDS             8850..9896
                     /locus_tag="MJB72_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBY8263170.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIATERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPNMKGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWENI
                     KSIAKEAVDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNLT
                     IDSNSIVVRGEVVG"
     gene            9889..10506
                     /locus_tag="MJB72_002064"
     CDS             9889..10506
                     /locus_tag="MJB72_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBY8263171.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEVTIF"
     gene            10518..11543
                     /locus_tag="MJB72_002065"
     CDS             10518..11543
                     /locus_tag="MJB72_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY8263172.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWVEDAKNGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEVRSETRS
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKLSGNLSDIATSV
                     NELITYC"
     gene            11560..13221
                     /locus_tag="MJB72_002066"
     CDS             11560..13221
                     /locus_tag="MJB72_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263173.1"
                     /translation="MATEWNFDFKAEAQPITLKAGKYKLECWGAHGKVWGGDSQSSGG
                     YSYGELTLKKETTLYVYTGATGSSNKYEKFTFNGGGLGVNNGGGGATDIRLVNGDWNN
                     EQGLLSRIIVAGGGGGAFSKTPAGKGGGFKGGNSTNDDNSSMLIVPGGTQYDGGRGYC
                     DEWDGVFGCGGGSILGLERGKYPYNSGGGGWFGGAGARNTSSGGGGSGYVLTKDSYKP
                     VGYIPTSEYWLENVGSITGGNTAKVNGYAKITLLQALPILTISSYNSTQATFKADHTD
                     PTLLTKIEYFIDDVLKETITTDLTLEKTINYTLEDNALHTLKIVVTDSNNATAEKVLS
                     ISKNIMPLPGENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELSE
                     KIKTSFDSSDASVQDLMNQLTRANNTISQLNTRYKVASGITYQLNNPSLSANFYNGGY
                     TTTQDHWINVSNLGFVPHIFIAECDFTKDGYLTKSLVFASYNVFSKDYVISSYFRRQT
                     NSTFYSHGNIYNLNEKDVYVNGRGVQLPAFNNYDFAYKWQAIKFV"
     gene            13235..13528
                     /locus_tag="MJB72_002067"
     CDS             13235..13528
                     /locus_tag="MJB72_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263174.1"
                     /translation="MNRANRIIYDQTGKMLLQTGEATGDVLEYDTITELHCIDIPYGS
                     IDYTKNRIIGINIETKEPILEEIPIFVTEEEKRIQELENQLLLNENEKVGGLL"
     gene            13528..13710
                     /locus_tag="MJB72_002068"
     CDS             13528..13710
                     /locus_tag="MJB72_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263175.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNREFELDDVTNVEYRKAVED
                     YIIRESGVVEEAEPTI"
     gene            13743..13934
                     /locus_tag="MJB72_002069"
     CDS             13743..13934
                     /locus_tag="MJB72_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263176.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENLFESDNVEGALKELATE
                     VNGQRAKGITIANDLIDMI"
     gene            13948..14580
                     /locus_tag="MJB72_002070"
     CDS             13948..14580
                     /locus_tag="MJB72_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263177.1"
                     /translation="MTEKLTDNASLRELMTTLQGVQTDFQNGKSNITTVLGSPFLNTD
                     KLDITKTKIETLKSALVNNLIYKGVSASTQSSFTDLINAINFIVQSILIVKCKGTGRI
                     EEVNNPYVIYNDKPNIKGCLRIKGTLEVYKNWATFASARLEIIYGEKKEYIYLSCDAT
                     ASGRSTNFEKDIIVNNDTQLKIQVLLTDVGTGNSSTAKAYTSVSDVTLLR"
     gene            14591..14794
                     /locus_tag="MJB72_002071"
     CDS             14591..14794
                     /locus_tag="MJB72_002071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263178.1"
                     /translation="MKKQFYYDSLEEKEKIINENNNLYVIELSETLKGKYIILSDNPI
                     YEKLSYEELENELLTNELKGEIL"
BASE COUNT         5784 a         1661 c         2823 g         4526 t
ORIGIN      
        1 cattgtttat ataaaaaaaa tggaaaaata tgtaataatt atatgttata ataattgtag
       61 caaggataat aatcgaaagt gcgaagggtg attattttca tattaaacgc caaattccaa
      121 ataaggaagg aggtgaaatt atatgatagg ttttttatta agcatactag ctggtgttat
      181 atcagcttat atttatgaca aaataaaaaa tcacccagac gccaataagg gtgatttaaa
      241 aaaataatat tttcacttaa caactgaaaa taatcactct ttgtaggagt aaattatttc
      301 cttgctttta ttataccaca aattggtaca gatattcaaa aataatattt ttatgatata
      361 ataaaaatgt agagattttg cagtgagcga tatttgttac aaaatatggc ttaacacttg
      421 aaatctaaga tgttgagggt gtgtgataaa tgttatcaat tgcactactc atggttcact
      481 gcaaatttga gagagatgcg tatgtgtagg tattggaaat gccaagttta ttttggggtt
      541 ttagattaac tatatggaat gtaaatttat ttttttgtta tcttttaatt caacagcttc
      601 tttgttttag attaactata tggaatggaa atacaaaatt atttaaaaca aatccagcag
      661 aaaccttagt tttagattaa ctatatggaa tgtaaattca gaattaacta atgtgaattt
      721 tttagacatg tcatagtttt agattaacta tatggaatgt aaattgatag aattgaaagc
      781 tacttcgtca cttgataaag ttttagatta actatatgga atgtaaatga aaaatacact
      841 atttataata aatgctgtta aacccggttt tagattaact atatggaatg taaattttat
      901 cattgtgtaa tgctctttag taatagaacc tagttttaga ttaactattt ggaatgtaaa
      961 tatagttata atcaatatta cttccaagga cagtagaagg ttttagatta actatatgga
     1021 atgtaaattt tactgcattt ataccatcta caccttttat tttattgttt tagattaact
     1081 atatggactt aaaattaaaa atactgaaaa acacttactt ttatggtagg tgctttttta
     1141 tattaaaatc tatgtactta attgaaatat ttggtaaaat atgtaagaat tgtatgatat
     1201 aataattgta gcaaggaaaa taataattga aaagtgctaa gagtggttat ttccatattt
     1261 gaataccaaa ttccataacg gaaggaggtg taacagtatg gtgataaatt ttttattgag
     1321 tatactggct ggtgttatat cagccttcat gtatgagaaa ataaaaaacc actcaaaggc
     1381 caataagagt ggtttaaaaa agtaattctt taaatcaatt ttgatggaaa tagctactct
     1441 tgtataaagt aaattatttc cttgctttta ttataccaca aattggtaca gatattcaaa
     1501 aataatattt ttatgatata ataaaaatgt agagattttg cagtgagcga tatttgttac
     1561 aaaatatggc ttaacactta aaatctaaga tgttgagggt gcgtgataag tgttatcaat
     1621 tgcactattg cccgctcact gcaattttaa gagtattgta tatatgtagg tattggaaat
     1681 gctaagttta ttttggggtt ttagattaac tatatggaat gtaaatatcc attctttatt
     1741 gtctatatct ataattttaa cctgttttag attaactata tggaatgtaa agcaacaaga
     1801 acaacagttg attttacctt ttctagccaa cgttttatat taactatgtg gtatgtaaat
     1861 atctttatta agagggctat cataaagagg tgcatagttt tatattaact atgtggtatg
     1921 taaatatgac tctattttat tttttatggt cctacaattt ttgttttata ttaactatgt
     1981 ggtatgtaaa tttttctgca tcttctacta tgttatattc ttctaatggt tttatattaa
     2041 ctatgtggta tgtaaatgca catttaactt caagtcttag agtattatct gcaagtttta
     2101 tattaactat gtggtatgta aatttcgttc aacttttatc ttagcttctc ctgctacttg
     2161 ttttatatta actatgtggt atgtaaatgt gctatttcca actttttttt caagttctga
     2221 atactgtttt atattaacta tgtggtatgt aaattagtta gtcccatatc gttatggtac
     2281 tgcattaacg cgttttatat taactaagtg gtatgtaaat agtgtaaaag gcattttttc
     2341 aaattgtgca ttggtttggt ttatattaac taagtggtat gtaaaggcta atgtttgagt
     2401 atttaaaagc atctgagcaa acaattttat attaactatg tggacttaaa attaaaaata
     2461 attcaaaaac acttacaaat gagtaagtgt ttttttaatg aaaggaggtg ataataatgt
     2521 aaaaatttta cgtatatagt ataatagtgc tataaaataa gtattatgtg aggtgattgt
     2581 tgtgttttgt tcaaattgtg gttatgagat aactggtgca ggcaaatttt gctcaaattg
     2641 cggaacagct acattagcag ataaagttaa caatgatgat ttatttataa atgttcatgg
     2701 aaaagaatta aatctgacta atatttataa agaaactaaa ggagataaaa tcttagcaat
     2761 tgatattgca atgaagttac tagggctgga cataaaagag tgtaaaaata ttatatatcc
     2821 agcttttaaa gaattaagtg aaaaaataaa tatcgaagag gaaaaagaga tattgaggga
     2881 agatgagtat aaacaaacta atgtacttga agatgatgtt gctcgttgcc ctaggtgtgg
     2941 ctctgtttca ttgtctgctc ataagaaagg ttttggcata ggaaaagctg tagcaggggc
     3001 tactatagca ggaggcattg gtttagtagc tggaaattta ggagcaaaga aagttagagt
     3061 tacatgtttg agctgtggta aacagttttg ggcataaata ataaacactt actaatgtag
     3121 gtgttttttt atatggaaat ttatgaaagg agagtgagga aatggctaca atacaaactt
     3181 caataaagat tttcgacgga atgacaccag catttcgtaa tatgactaat tctattaata
     3241 caacaattaa tagtttagag agattgcaag gcagattgaa taatccactc aatgcaggta
     3301 atatacaagc ttctcaacaa agtttgaata acatagaaag tattctcaca aggatagaac
     3361 agaaaattgg aagaaataca aatgaacagg aaaactttaa taataaaata agacaaggta
     3421 gtgaagcagg ttctctatta gtgtctaaat taaaaagtat tgctgggata tacattggaa
     3481 taaaaggaat agatagtatt acaaaagcag cagatacaat tgcaagtaca aaagcacgtt
     3541 taaatctaat gaatgatggc ttacagacaa cagaccagct taataaaatg atttatttgt
     3601 cagcccaaag tgcaagagct agttatgcag atacagcagc acagattgct aaacttggaa
     3661 tacttgcagg agatgctttt ggaagttcag cagaggtgat aaagtttaca gaacttatga
     3721 ataaagcttt tgtaattgga ggaacatcag caaatgaagc tagtgcagca atgtatcaat
     3781 taacacaagc catgggtgca ggaaaacttc aaggtgatga gttccgttcc ataatggaaa
     3841 atgcaccttt attagccact aaaatagctg atgcaatggg aaaaactaaa gaccaattga
     3901 aggaattatc aagtagcgga gcaataacag cagatgttat aagaaatgca ctgtttaaag
     3961 cttctgatga gatagaaaag aaatttgcaa gtatgccaat cactttttct caagctctca
     4021 caatgatgaa aaatgatgct tatatgatat tttctgagac tctcagtaag ataagtggag
     4081 ctttgcaaag tgtacgtttt agtgagattg ttgtatctat gcggaatgtt atgattgcaa
     4141 tatcttcaaa catttatgat acattaaata ttataaaaaa tatattaaat agtgattttt
     4201 tttcagattt tgttagcaat gttacattag gagctatact aattatcaat ggactgggat
     4261 gggttactaa tgcaacacta aatgttgcta atgtctttgc tcaaaattgg tcgattattg
     4321 ctcctattat ttatggagtg atagcagcaa ttgcgatata taaaggagta ttacttgcaa
     4381 gtacaattgc cactacggta gcatcttttg taaattcatt gtatgcagta gcagcgtata
     4441 aatcttgtgc agctttagca gcacaagaat ttgcgatatt tggaaaaata tctgcacaaa
     4501 cgatggaagc tttggtaaca gcacaagcaa cagcagcaca atggggtttt aatgcagcat
     4561 tattatcctg tccaattttt tggataatag caggtattat agcatttgta gtagtagttt
     4621 ttgttgcagt agcagcagta aacaaattct caggaacaag tctgactgta ctaggagcaa
     4681 ttgtaggtgc agtatttgca gcagtagcat ttatacaaaa cataatgata tggctattta
     4741 atagatgcgt agatgtaaat gaagggattg caaatggatg gaatcaatgt gtatatctaa
     4801 tgaaacaagc tattgcaaaa ggtgtaatct ttataattga gaaaatggca tcattaaatg
     4861 atgctgtaaa taatgctgga aatgcacttg ggaaagcttt catagatggg gcaaatatcg
     4921 caatacgagg tgtaaacaaa ttaattgacc taataaataa aataccaggg ataaatattg
     4981 gtaaagttgg agaagcaaca tttacgcctg tcaaggcaga taatagttac atcaaacaac
     5041 agattgacag tttaaataaa tgggtaggag atgcaccaga aaaaataaaa ttggatagaa
     5101 tgggatataa agatattgga gcagaatttc aaaaaggaaa tgcacttgga actaaatgac
     5161 aaaatgctat aactgataaa tttaaagata cttttgacat taataagatg ctagaagatg
     5221 caaagaaaaa attaggatta gacgatttgt ggaataaaca aaatccttta aacaaccttg
     5281 gtggatttgg tggagattta ggaaaaaatg taaaagacac agcaggaaac acagccaaaa
     5341 tggctaaaac aatggataaa agtcaagaag acttaaaata tcttagagac attgcagaac
     5401 aggaaacaat aaaccgtttt acaggagtca acattaaaat tgacatgaac aatacaaaca
     5461 acataaatag tgaggcagat gtagatggaa tagtaaatgt actaacagaa aaattaaacg
     5521 acgctatggt tgtatcagct gagggaatag tttagagagg agggatataa atggcttatg
     5581 acttttattt agatggagta caattaccaa tacctccgcc aaagttagag attaaagtta
     5641 caaataaaaa caagacagtt gatttaataa atgttggaga agtaaatata ttaaaaaaag
     5701 aaggattatc tgaaataagt tttgaagcag aatttacaca taataaatta cctttttgta
     5761 gaggtcaatt tagagatgtt caattctttt taagtaaact agaattacta aaaactgatt
     5821 gtaagccatt ccaatttatt gtctcgaggg aaatgggtgg aaaagtacta tttaacacta
     5881 atatgaaagt gtcattagaa gaatataaca tagtagaaga tgcagagaat gcctcagata
     5941 caaaagttgc aataaagtta aaacaatata gagattactc aactaaaaag ttagttcctg
     6001 ctccacctaa aaaaactaat gagactggta gaccaagcgt aaagatagag ccaaaacgag
     6061 ttgattcagt caatgcacca agtaataaga cttatacagt caaaagtggt gattctcttt
     6121 ggtcaatttg tcagaagcaa cttggtaatg gttcattata taagaaggta tatgagttaa
     6181 ataaaacaat gatggataag gctaacaagg gtaaaaaagt acctaaatac accatctata
     6241 aagggcaggt gttgaggctt gtctgatgat ttagttctgg cgaatgatag agatataagg
     6301 ctagttattg cacattggga agatttctac gaacctgctg tcattgatgg tatcacatgg
     6361 gaaatagaaa gacgaggaac accatctaaa cttgaattta caatagtcat ggatgatata
     6421 ttagagtttt gtgaaggtaa ctctgtaaga ctatattaca aaggtgtagg tatattctat
     6481 ggatatatat ttcagaaaaa gagagataaa gaaaatcata tcaagatagt tgcctatgac
     6541 caattaagat tttttaaaaa taaagatact tatgttcttg agaaccatac tgcaagtgaa
     6601 cttgtaaaga tgttagctaa agattttaaa ttaaaataca atgtcataga agatacaaaa
     6661 tataaaatat cgagagttga agaaaataaa acactctttg atatggtctt aactgcacta
     6721 gatgatactc taagagagaa aaaggaaatg tatgtactct atgatgattt tggaagatta
     6781 acattaaaga atgttgcatc aatgaaactg gatactgtca tgaacaatga tgtcatagag
     6841 gactttgact ataattcaag tatagatagt gatacttata caaaaatcaa acttgtaaga
     6901 gacaacgagg agtcaggaaa aagagatgtg tatattgctc aagactctac acatatgagg
     6961 agttggggaa tactccaaat gtttgagaca gttgataaaa atatgaatga agcagagata
     7021 aaacaaaagt gtgatatact tctaaaacta tataataaga aaactaagtc attaagttta
     7081 aaaaatgcac ttggagatat tagagtgaga gcaggttgtt tagtacctgt ttttttaaat
     7141 ctaggagata ttaaacttca aaattatatg ctagttgaga aagtaaaaca tacttttgaa
     7201 aataacagtc actttatgga tttgactctt gttgatggag atgaatttgc ttcttattct
     7261 tcaagctcat attcaagtgg aaatactaac aataaggatg aaaagaaaaa tggtcctgca
     7321 caaagtatta cgaaaaaaaa tacaggtaaa aaagttcctg ctatatttac tgcatattat
     7381 ccagggaaca atgcaatgga aggcggaaaa acagattgca atggaaagcc acttgatgta
     7441 aaatcaagaa ctgttgctgg tccaatgaat cgagaaggag ttaagaaaac ttggtatact
     7501 gatgattttc taaataaaca tccagttttt gaatatggag ataaagtaaa aattatactt
     7561 cctggtactg cctatgacaa caaagtatat acagttaaag ataatggagg aagaatatat
     7621 gttgaaacaa acggaacata tcatatagat atactattag ctaatgctag tgaatgtaaa
     7681 aaatttggta gaaagaatgg ctatataatt ataggtggag atgaagaaca aacatatcaa
     7741 gttgaaggta ataaccaaag taatacaaat aacaattcta aagaagataa attaattagt
     7801 atagcaaaaa gtaaactggg ttgtaattat gtgtatggag cagaaggtcc taataatttt
     7861 gattgcagtg ggtttactca atggtgttat aaacaaatag gtataaaaat tcctcgtact
     7921 gcttctgcac aaagtaaagc aggaaaagca gtagatttaa aagatagaag caagtggaaa
     7981 gcaggagact tattatgtag aattggtgga ggaagtagta atcatgttgt aatgtacatt
     8041 ggaaacaatc aaataattca ttcaccacaa acaggagatg tggtaaaaat agagtctgtt
     8101 aattcttata gaaaaggaaa agcatacaca catgtgagaa gatttatata agtgaggtgg
     8161 caatatgagc caagatttat tacagataat aaaaaaagct gcaatggatg cagtagaaac
     8221 aagcaaccca atgagggttg tatttggaac aatagaaagt attagtcctc taagagttaa
     8281 gatagaacaa aaactatcta ttggtgaaat ttttctaata caaacagata catttaaaag
     8341 atatacagat aaaaaaatag gagataaatt agtcttaatt cgtatgcaag gagggcaaca
     8401 atacttgatc ttagatagga tgtgataaag tgttaccaag tgataattta gattatgata
     8461 ttgaagatgt atcaataatt aattttgatg taagacaaga gccaagtaag acatttaaac
     8521 ttcatataga aaagtctaag attgatggta tttgtgatga tgtagaagca ttaaaacaga
     8581 ccatcttttt gattttaaac acagagagat accaacatct aatatatagt tggaattatg
     8641 gagtcgagtt gaacgacctt attggagagc ctatatcctt tgtaatacca gagttagaga
     8701 gaagaatcaa agaagcacta attcaagatg atagggttga aaatgtagat aattttgagt
     8761 ttgaaaatat aaagggtaaa gtacattgta agttcacagt ttacagtaaa tatggaaata
     8821 taaaagcaga gaaagtggtg agtgtataat tgtttgagtt aatgacattt gaaaatataa
     8881 ttaaaagaat gttagatagt gtaccagata cttttgataa aagggaaggt tctataatat
     8941 ataatgctct tgctcctgtt gctatagaac ttacagaaac atacattgcc atggatgaat
     9001 tactagacca aacttttgta gatactgcta gttattacta tttagagaag agatgtaaag
     9061 aaagaggaat cacaccactt gaagccacta atacaattgc aaaaggagtt tttaacatag
     9121 atattccact tgattcaaga ttcaacttag gagagtataa ttatattgca actgaaagga
     9181 ttagtgaagg tatatataag atgaaatgtg aaactgctgg acctattttt gagttggggc
     9241 aactaatacc tattgaatat atagatggtc ttgaaacagc agagttaaca gaaatactaa
     9301 taaatggaga agatgaagaa tctgaggaca gtttaagaca aagatattat gatagtttaa
     9361 attcccaatc ttttgggggt aatatacaaa actataagga tgaagttaat aaattgcctg
     9421 acgttggggg agttaaagta tatccagttt ggaacggtgg aggtactgtt aagttagtaa
     9481 taattaattc taacttcaaa gttccatcta gtgatttagt taatttagtt caagaagaaa
     9541 ttgacccaaa tatgaaagga gaaggtttag gattagcacc aataggtcat agagtcacag
     9601 ttgaaggagt tacaagtaca actataaata tatcagcaga aataacatac aagagtggtt
     9661 acacttggga gaatataaaa tcaattgcaa aagaagcagt tgatgactat ttaaatgagt
     9721 taaatatgag ttgggaagat gaagaaaact taatagtccg tatatctcag atagaaactc
     9781 gtttgttaag tatagatgga gtattagaca ttgcaaatac aatgataaat gaggttaaat
     9841 ctaatctaac aatagatagt aacagcatag tagtgagagg tgaggtagtt ggataaagag
     9901 attaatctaa taaattactt accacaaatt ctacaagata aagaagaata tataaaagta
     9961 tttaatgtag gaaataaaga aataaaaata ttacatgata aattaaagga cctatcaaat
    10021 gaccagtttt tagaggacct aactataagt ggtataaaaa gatgggaaaa gataatgtct
    10081 ataactccta aaagtaatga gagtttagaa gatagaaggt ttaggatttt tagtaaatat
    10141 ataagtaaat taccttactc agagagattt ttaaggaact ggctagataa tgtagttgga
    10201 gaaggcaatt atgaattaac tattaataat gctacttata acatacacct tgagagtgat
    10261 gctagaaatc aagattggtt tgaggaagtt cattcttttg taagtaatat taagccatgt
    10321 aatatgactt tagattacac tagagtgctt ataagcaaag acaattatat gaattttggt
    10381 ataacaacct taatgggtca agaaataact atataccctt ggagtccacc agatatagaa
    10441 acttatggag aaattgatgt attaactggc aatggagttg gataccaaga ggtaacaata
    10501 ttttaggagg tgatatattg gctatagata aaagttatta cactataatt acagatgtag
    10561 ggaaagcaaa gatagcaaat gcaagtgtca caggtaataa agtgggattt gtaaaaattc
    10621 aacttggtga tggaggaggg agtgaatata ctccaactga gagtcagaca gctctcaaaa
    10681 atgtggtatg ggaaggcaat atcggaaata caactacaga tgaaactgca ccaaattgta
    10741 taatattaga gagtttaata ccatcaagtg taggcgggtt tatgataaga gaaataggat
    10801 atttagatga tgaaaataat ttaattgcca tttctaaata caaagagtgt tataaacctt
    10861 ctatagaaca aggtgcagtg gtagacatga aggttaaaac tgtgcttatt gtatctaatg
    10921 taaataatat agaacttaaa attgacccaa caataatctt tgcaacactc aaagatatac
    10981 aagacttaga aactaaaata ggtactgtta atactaaaat tgatacaact aaaacagaat
    11041 taacaagcaa catagaaact actaaaacag agctaaacac tagaattgac acagaaaatg
    11101 agaaacaaaa tattaaaatt gaccaactta ttgcaggtgg ctctaatgtg gcatctactc
    11161 aaacaataac aattgacgat tgggttgagg atgcaaaaaa tggattcaaa gcaactgtaa
    11221 cacatagttt attaacacag agaatagttg taaatattat agatgctact acaaaagaaa
    11281 atgtagttac aaactttaaa attatagatg ataattctat agaagttaga agtgaaacaa
    11341 ggtcagaatt aaacgtttat gtgataaatg gaaatgcaga aactcatttt ataaatgcga
    11401 ctgtagatga taacagagta tctgaaatga ctacttattc gtctaagaaa atcgaagata
    11461 gattggttaa tatagaagaa aaattgagtg gaaatttatc tgatattgca acaagtgtaa
    11521 atgagttgat aacttattgt tagagaggag agtgagaaag tggctacaga atggaatttt
    11581 gattttaagg cagaagcaca accaattact ctaaaagcag gtaaatataa attagagtgt
    11641 tggggagctc atggaaaggt ttggggtgga gattctcaat ctagtggagg atactcttat
    11701 ggggaactta cattaaaaaa agaaactaca ttatatgtat atacaggtgc tactggttct
    11761 agtaataaat atgaaaaatt tacatttaat ggaggaggac ttggtgttaa taatggagga
    11821 ggtggagcaa ctgatataag acttgtcaat ggagattgga ataatgaaca aggtcttttg
    11881 tctaggataa ttgttgctgg tggtggtgga ggcgcattta gtaaaactcc tgctggcaaa
    11941 ggtggtggtt ttaaaggcgg aaatagtact aatgatgata acagttcgat gcttattgta
    12001 cctggtggta cacaatatga tggaggtaga ggttattgtg atgaatggga tggtgttttt
    12061 ggttgtggtg gaggttctat tcttggttta gaacgaggta aataccctta taattcaggt
    12121 ggaggtggat ggtttggtgg tgcaggtgct agaaatactt caagtggtgg aggtggaagt
    12181 ggctatgtat taactaaaga tagctataaa cctgttggat atatacctac atctgaatat
    12241 tggttagaaa atgttggtag tattactggg ggtaatactg ctaaagtaaa tggttatgct
    12301 aagataacat tactgcaagc attaccaatt ttaactatat cttcttataa ttccacacaa
    12361 gcaacattta aagcagacca cacagaccct acactattaa ctaaaataga atattttata
    12421 gatgatgtac taaaagaaac tataacaaca gatttaactc ttgaaaaaac aattaactat
    12481 acattagaag ataatgctct acacacactt aaaatagtcg ttacagacag taataatgct
    12541 acagcagaaa aagtattaag tataagtaag aatataatgc cattgcctgg agaaaatgta
    12601 aatttaaatg atatatcaac aaaattagtt gaggttaatg caggatttaa agttgggaaa
    12661 acaagcatta taaacacttt agcattaaaa aatatagaag caagtttgaa taacacactg
    12721 gtggaattat ctgagaaaat aaaaacaagt tttgatagtt cagacgctag tgtacaagat
    12781 ttgatgaatc agttaacacg agctaataat actataagtc agttaaatac aagatataaa
    12841 gttgcgagtg gaattactta tcaactcaat aacccaagtt tatctgccaa tttttataat
    12901 ggtggttata caactactca agaccattgg attaatgtta gcaatcttgg ttttgttcct
    12961 catattttta tagctgagtg cgattttaca aaagatggtt atttaactaa gagtttagtt
    13021 tttgccagtt ataatgtttt tagtaaagat tatgtaattt cttcatattt tagacgtcaa
    13081 acaaattcaa ctttttactc acatggcaac atttataatc ttaatgaaaa agatgtttat
    13141 gttaatggca gaggtgttca acttcctgct tttaataatt acgattttgc atataagtgg
    13201 caagctataa aatttgtata aatggggtga taaaatgaat agagcaaata gaataattta
    13261 cgaccaaaca ggcaaaatgc ttctccaaac aggagaagca acaggggatg tattggagta
    13321 tgatacaata acagaattgc attgcattga tattccgtat ggaagtatag attatactaa
    13381 aaatagaatt ataggtataa atatagaaac aaaagaacca attttggaag aaataccaat
    13441 attcgttaca gaagaagaaa agagaataca agagttagaa aaccaattat tactaaatga
    13501 aaatgaaaaa gtaggaggat tattataatg aatataaata atgttgtagt aagaatatta
    13561 gcagagagga ttttaagtag aggcttaaat cctctaaaaa atcgagaatt tgaattagat
    13621 gatgtaacta atgtagaata cagaaaagct gtagaggatt atataattag agaaagtgga
    13681 gtagtcgaag aagcagaacc aactatatag agggttcttt tttattgaaa gaaggtgact
    13741 aaatgacttt taaagagtta gttaataaag ttagaaatct tgtattagaa gcaaagaatg
    13801 taactataga agatacagaa aacttatttg aaagtgataa tgttgaggga gcattgaaag
    13861 aattggcaac agaggtaaat ggacagagag caaaaggaat tacaatagca aatgatttaa
    13921 tagatatgat ataagcgagg tgaaaatatg acggaaaaat taactgataa tgctagttta
    13981 agagaactta tgacaacact acaaggtgta caaactgatt ttcagaatgg caaaagtaat
    14041 ataacaactg tgttggggag cccattttta aacacagata aattagatat aactaaaaca
    14101 aaaatagaaa cattaaaaag tgcattagtg aataatttaa tatataaagg agtgtcagca
    14161 agtacacaaa gttcatttac tgatttaatt aatgcgataa attttatagt gcagtcaata
    14221 ttaattgtta aatgcaaggg tacaggaaga atagaagaag taaacaatcc gtacgttatt
    14281 tataatgaca aacctaatat caaaggttgt ttaaggatta aaggaacttt agaagtttat
    14341 aaaaattggg ctacttttgc aagtgctaga ctagaaataa tatatggaga aaaaaaagaa
    14401 tatatttatc tttcatgtga tgctactgct agtggtcgtt ctacgaattt tgaaaaagat
    14461 ataattgtaa ataatgatac acaattaaaa atacaagtgt tattaacaga tgttggaacg
    14521 gggaatagtt ctactgcaaa agcttacaca tcagtttctg acgttacact gttaagatag
    14581 gagattaggt atgaaaaaac aattttatta cgattcttta gaggaaaaag aaaaaataat
    14641 taatgaaaat aacaatttat atgtaataga gttatcagaa acattaaaag gtaaatatat
    14701 aattttgtca gataatccaa tatacgaaaa acttagttat gaagaattag aaaatgagtt
    14761 gttaacaaac gaattaaaag gcgaaatttt ataa
//
LOCUS       DAGSHT010000077        14762 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGSHT010000077 DAGSHT010000000
VERSION     DAGSHT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.079"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            765..1526
                     /locus_tag="MJB72_002072"
     CDS             765..1526
                     /locus_tag="MJB72_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY8263179.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGLPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1591..2373
                     /locus_tag="MJB72_002073"
     CDS             1591..2373
                     /locus_tag="MJB72_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBY8263180.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2640..3059
                     /locus_tag="MJB72_002074"
     CDS             2640..3059
                     /locus_tag="MJB72_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBY8263181.1"
                     /translation="MDKISGTLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(3162..3428)
                     /locus_tag="MJB72_002075"
     CDS             complement(3162..3428)
                     /locus_tag="MJB72_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBY8263182.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3655..4356
                     /locus_tag="MJB72_002076"
     CDS             3655..4356
                     /locus_tag="MJB72_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263183.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4438..5046
                     /locus_tag="MJB72_002077"
     CDS             4438..5046
                     /locus_tag="MJB72_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY8263184.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5370..5834
                     /locus_tag="MJB72_002078"
     CDS             5370..5834
                     /locus_tag="MJB72_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBY8263185.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            6471..7487
                     /gene="gap"
                     /locus_tag="MJB72_002079"
     CDS             6471..7487
                     /gene="gap"
                     /locus_tag="MJB72_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBY8263186.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            7882..9051
                     /locus_tag="MJB72_002080"
     CDS             7882..9051
                     /locus_tag="MJB72_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263187.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            9432..13685
                     /locus_tag="MJB72_002081"
     CDS             9432..13685
                     /locus_tag="MJB72_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBY8263188.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            13834..14328
                     /locus_tag="MJB72_002082"
     CDS             13834..14328
                     /locus_tag="MJB72_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBY8263189.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            14444..>14762
                     /locus_tag="MJB72_002083"
     CDS             14444..>14762
                     /locus_tag="MJB72_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBY8263190.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVAC"
BASE COUNT         6002 a         1470 c         2622 g         4668 t
ORIGIN      
        1 agtatttaca gataatgtag attgcacagc tataagttag ataatagaat tatgtaatca
       61 agaatttaca acaaagtata agataaggat aatgctaaat tgccatacag gtgctagatg
      121 tgtaaatgga tttaattaat actattgata aagtgcaaca agcacataaa ccaattgatg
      181 agattattaa aactataaga gatatagttg acataattaa aatattggaa cctatttata
      241 attttaaatc aaatcaataa tgattaagat taaaataata ttaaggcatc aatgttataa
      301 gagtacaata taaggagtac tatatagtac tccttactta tacatattag acaataggga
      361 atttttttat agttaaattt tcaaatattt tttagggaac tttataattg agaattaagg
      421 gtatagtatg tgatgaactt actaaattta taattaggtt atataatgta ataatcatca
      481 atagttttac tttagggaaa atatatgctt aaggaagtat agcttactaa tattattaaa
      541 atgtatattt aaaatgataa aaaaagaatt agacaagtat ttagtaaatt gattatgcaa
      601 aattctatca agcaggaacc ctatgacttt agtcatggga ggttcagtag gtaacttgtt
      661 gaattaagtt tatattattt aaataaacta cttaatttag ataatataaa aagtcagaat
      721 tttaagaata gagctatata atcttagcta ttaggagaat tactatgata ggaatatatt
      781 ttagtggaac aggaaattca agatactgtg tagaaaaatt tttacaagaa tatgatataa
      841 cagcaaattc ttattctatt gaagataatg aacttttaca gcacatcaat aatcatgaga
      901 atattatttt tagttatccg gtgcaatata gtaatgtgcc aaaaatattg aaagatttta
      961 taataaatca ttcaactttg tggaatggta aacgtgtttt tattattgca actatggctc
     1021 tatttagtgg agatggctct ggtgtgttat ctagaatatt aaaaaaatac ggtgctataa
     1081 ctgttggtgg attacatgtg aagatgccag atagtatagg agatgaaaag gtattaaaac
     1141 attctttaga gtacaataaa aagcttgtaa taaaagcaga aaagaaagta actaaagctg
     1201 ctaaaaaact gaaacatggg ttaccaccaa aagaagggtt aggttttttg agtcatattg
     1261 tagggttatt tggacaacga ttgtatttta tcaataaaac aaatatgtat acagataaac
     1321 taaaaattaa tactgaggaa tgtattgtat gtggtaaatg tgttaattta tgtccaatga
     1381 agaatctagt aattaaaaat tgtatgattg ttgcaaatgg gcaatgtaca atgtgttatc
     1441 gttgtgtaaa taattgtgcc aaaaaagcaa ttacattgtt aggtaaaaaa gttgttgaac
     1501 agagtaatat taaaaaatat atgtaagaaa tttaaagttt tattgaatgt aaattgatgt
     1561 atattttatg ttagaaagag aggttaaata atggaatact acatagttga ttcatttgca
     1621 actaaactat ttaaaggaaa tcctgctgga gtttgtgttc ttgacagaag aataccttta
     1681 gagttaatgc agaaaatagc agaagaaaac aatttaccag aaacagcatt tgtagttaaa
     1741 ggtaaaggaa attatgaatt acgatggttt actcctaaag cagaaattga tttatgtggt
     1801 catgcaactt tagcagcagc ttatgtaata tcaaatttta ttgatgttaa tgtaaaaagg
     1861 atagatttct ttactcaaag tggaaagctt gaagttacac gaaatggaaa tctatatgaa
     1921 atgatatttc cagaaataat gcctattgaa atagagttat caccacaaca agctaatttg
     1981 ataggctgtg taccaagtgc tgtttattct tcaagagatt taatcttatt gctaaacagt
     2041 gaacaagaag ttataaatta taaacctaat tacgcacaat tgcgtaaatt gacagactgg
     2101 ttaggtataa taatcacagc acaaggaagt aatacagatt ttgtatcaag atatttttgt
     2161 ccagaattag attcagaaga ccctgttact ggttcatcac attgtaatct tattccatat
     2221 tggtctgaaa agttgggaaa acacaaaatg gtagctgctc aactttctaa tcgtggtggt
     2281 ataattcagt gtgaagtgtt gaaggataat actgtgaaaa tttctggtga agcagtcttg
     2341 tttatgcagg gaacaataaa gattgatatt taaataagta taataactaa tttattatga
     2401 aaaataatta attttcttag tgttaacaat aataaagtag agttaaaaaa cttgatttca
     2461 cttaaatcaa tatgatagat gttgatacta agattgacaa gcatgattaa aaaagataat
     2521 atttaatcta taaagataaa aacacagtct ggttggtagt ccagacgcag caatagctgt
     2581 cagtaacctg cctccttggt tgtccgttct ttagaaagta atttaaggag gaactagcaa
     2641 tggacaaaat tagtggaaca ttaacagtct tatttgaaga accattttgg gtaggtatat
     2701 ttgaaagaca agatggtaaa aaatatgaag catgtagggt agtttttgga gcagaaccta
     2761 aagaagtaga agtgtatgaa tttatacttg aaagattttt ctcattagat tttggtagca
     2821 ttaaattaga aaaaaatgtg accaaagaca acattggata caaaagaatg cagagaaaag
     2881 taaaaaagga acaagaaaaa gaaactatag ggactaaggc tcaaaatgct cttaagcttc
     2941 aatatgaaga aagaaaacag gatagaaaaa acctagctaa aaatagaaaa gaagaagaaa
     3001 aagaaagact atttaatttg aaacaagaaa agagaaaagc taaacataag ggtcattaat
     3061 tttaattaaa tatcttttta ataattgcta gaaaaaggtg tataaaattt ttaattttat
     3121 acaccttaat ttattgaaaa gaataaggtt ttatatttca attactttaa aatataaaaa
     3181 tatactaata aaaattgtaa aaatagtata aatggtatac caaatgtaaa tttaatgtgt
     3241 ttagttttat gtctaaaaga atacattcca attatagaac ctatactacc tccaattact
     3301 gctatagaca taagagtagc ttcttttatt ctccattcgt ttctaattgc tcttttctta
     3361 tctatgtaca ttgaaaagaa agcaatagaa ttaatcacta taaaatatat tagaataaaa
     3421 tttttcacag tcttgcctcc ataaaaatat attatacatt ataaaacaag atatttgttt
     3481 tggaaagtta aatttgtaat tagtacatat aaaatatttg ataagaacaa atataatatt
     3541 aattggaaaa tttaaaaaaa ttaaacaatt agcaaatagg gggtatattt ataagacaca
     3601 aagtgataaa atctatttag aaaataatgt aaaataacag gaggataaag caaaatgaaa
     3661 tttatagtag aaaaagaagt ttttgataaa ttagaaaatg tatgctttgg tgtagtagtt
     3721 gcaaaaggaa tagataatac taaagaaata gaaagaataa gcaacctttt agatataagt
     3781 atagatagag tagaagatta cttcaaagat aaaaaagtaa aggaatcaga agaaattata
     3841 ccatatagag aggcatttag aagtctaggc atgaacccaa ataaatttat gagttcgata
     3901 gaagcaatga caacaagggt tgctaagaat aagaaacttc ctcatataaa tcctattgta
     3961 gatttaggaa actctatttc tttaaaatat ttacttccaa tgggtgctca tgatatggat
     4021 tttagaaatg atgatgtata tgttagattt tctaaaaaag gggataaatt tgtaccattt
     4081 ggagaaactg atgttgaatt aatggaagaa ggagaattaa tttattcagt tggagacatg
     4141 gtaaaaacta gaagatggat atggagacaa ggagaggaag gaaaaattac taatagctca
     4201 aaaaatatat tttttcctat agatggtttt actgatgcta atttagataa ggtaatgagt
     4261 gcaagagaag aattagcaaa attgttaaag gaaatattta attgtgaaat taaagtaggt
     4321 tttgtagata aagataatcc tgaaatggaa atataataag ttgttgaata cttgttaaga
     4381 tattatattt acttaatgat gttaaaaagg tattaaaaaa gaaaggtcgt agtaaatttg
     4441 aaaaaaacta taataaagaa tgtaaatcca atgattgaaa ataagtttat aggacttttt
     4501 gaaatagagt ataaaaacaa attagatgaa gataaggttt ggatggtagc atcaagaaaa
     4561 agtagtgagc aattaaaaaa tatttatttg aagaatgaag aagatagtgt agatgcagtt
     4621 gtaatagttg ggcttcataa aagtagtaaa aaattaattt taataagaca atttagagta
     4681 cctattaatg gatatatata cgagttacca gcaggtcttg ttgatgaagg agagtctata
     4741 gatatttccg ttgaaagaga acttagagaa gaaacaggac ttactctttt agaaattaaa
     4801 aaaaataaaa gtattgataa agtttattta tctcctggaa tgagtgatga atctgttgcc
     4861 tttgtttatt gtatatgtga tggtgatata acagatgaat ttttagaacc agatgaagag
     4921 attgaagcgc ttttaatttc gcaagaagat gcaaaagaga ttttacaaag taatcataag
     4981 atagacacaa aagcattttt gattttacaa atgtttgtga gtttaggagc taaattattt
     5041 gaatagatat atagaaaaga ggttatatag aagagtaaaa taataaagtt tattttactt
     5101 gtataaatta taatctcttt ttttatacaa taaagtataa ttttgtgtat ttttaaataa
     5161 gtgacttgac tcttaaattt tcataatatg ttataaacca atttaagata ctacaataat
     5221 taaaattcca aaattattta acatatttgc aattaaaatg agaaaaggta aaggctgaat
     5281 gattgaaaaa tttgatgtaa aatagtagct tagtttaata ttatagtttg tagggttaaa
     5341 aattagaaat tgtaagggag ataaattcta tgaaaaaatt agttattatt ttaattagta
     5401 tcattagtat ggttagcttg acagcttgta aaaaaagcaa tgaaagtaaa tataataatt
     5461 taaacaacaa agaatccgga caaacaacca ttgaaatgga cttagataaa aattatgata
     5521 cttcagaccc atttgttaat gcacgtttgt tttgtgtgtc taatgatata gatgttttag
     5581 acacagagat ttcttttaaa atggatggag atagtggtat tgtggaaata aaggacaata
     5641 aaacatatga aactttatgg agtaatacat ggcatggaag cgttgataat gatacattca
     5701 caatttctct ttctaatata caaaaagaga aagagtatgc tatatgcttt actggaatta
     5761 aaataaatca tgcagttgta aaggttactt ttgaaagcaa tttagttaag gagaaagaaa
     5821 aaccctcaaa ataaattcca gattgtctat aacttgatat tcagtacata aaagcagttg
     5881 atatttatga ttgactgctt tttcttatca aaaaggagag tttgtgcatt tttacttgaa
     5941 atagtttatg cacatcaaaa gttggtgagc atgatattgg aaatggtaag catgaaatct
     6001 tgataagaca aggaaataaa tttaagtttt caattaagta tatttccata atttagttaa
     6061 caataagcta attattattt atattttgat atataaataa taattgtagc aaatttcaaa
     6121 aaatcatata ttttttgaac taaataaaaa tttattttta ttattttaaa ttagaggaga
     6181 ttcttgaaga tatattattt ttaaaaataa gtatagttta aaaattcaaa aaaaagatgg
     6241 caaaatatgt cccaagggac attgagtaaa ataaatgatt tcagcttatt tacactaaaa
     6301 agtacatact aaatgagggg taaaataaag aaaaatgata tactaattgt cttttgataa
     6361 taaaaaaaca tggaatttga ttatttaatc aagaaaaagt attgaacaaa tgatttattt
     6421 agtgtacaat aatcatgtaa aaacatagta atggagggag acatttcata atgaaaatta
     6481 aggtaggaat gaacggattt ggaagaatag gaagagctgt attaagaata gctcaagaag
     6541 aattaggaga taatatagaa attgtagcca taaatgcaag agcaacaaca gaatctttgg
     6601 cacatttatt tacatatgat tcatgctatg gaacttttag aggtgaagta gaggcaaagg
     6661 atgaagacac tctagtagta aactctaaag agataaaaat attaagatat aatgacccag
     6721 aagaattacc atggaaagaa ctgggggttg atatagtaat agaatcaaca ggattattta
     6781 cacaaagaga aaaggcagaa aagcatatta aagcaggagc aaaaaaagta attataacag
     6841 ctccaggaaa aaatgaagat ataactatag ttatcggagt taatgagaaa gaatatgata
     6901 atgaaaagca caatataatt tcaaatgcat cttgtactac aaattgtcta gctccttttg
     6961 ctaaagtatt ggacgaaaaa tttggtatag taaaagggtt aatgactaca gtacattcat
     7021 atactaacga tcaaagaata ttagataaaa gccataaaga tttgagaaga gcaagagcag
     7081 cggctgaatc tataatacct accactacag gagcagcaaa agctgtgtca agagtactac
     7141 cacaattaga aggaaaatta aatggttttt cacttagagt accaacacca acagtttcac
     7201 ttgttgattt agtgtgcgaa cttaaagaaa acgttacatc tgagcaagtt aactctgtgt
     7261 taaaagatgc tgctgaagga gaattaaaag gtgttttagg atattgtgac aagccacttg
     7321 tttctataga ttataaagga gattcaagat catctattat agacgctcta tccacaatgg
     7381 ttatagaaga caatatggtt aaggtagttt cttggtatga taatgaatgg ggatattctt
     7441 caagaactgt tgacttagta aaatatgtag ctgaaaaatt aaaataatat atatttctta
     7501 aagcaaattt taaaaaaatt ttttaaaaaa attatataaa actaaaaaaa aagattatga
     7561 aaaatattga aggaaaaatt ttttcataat cttttttctt tcatgcaaaa agatacaagt
     7621 tacaacttat atttgcatat taggtcgtta gagaagtata tatatattta gtaaggaatc
     7681 atacttttta taaaatgaaa gataagatgt cgaaaacttg cataaatctg aaaaattttt
     7741 attattaaaa tactgatttc cattttaaaa atgataaaaa aatgacaaat aaagttgcaa
     7801 atagtaatac ttataagtat aattaaaaaa gagaaaaggt tttgttaaac tttataacaa
     7861 aaaaagaagg gatgattcaa aatggagaag ttaacagaca ggagacaggt taaggcagta
     7921 agcatggaaa ccttcatttt cctaatatta ctagtagttg gttttgggta tgttggtagt
     7981 attatgggag caggaatgat gttcaaagta ataatgagta cagcacatgc tttactattg
     8041 gagacagttt tcttaattat ggctatggca gttcttgcag gcgcattaag cgcactactt
     8101 tcagagtttg gagtaatagc gttaattaat aaaattttcg ctgtatttat gaagccttta
     8161 tatggactac ctggtgctag tatagcagga gctataacaa cttatttatc agataatcca
     8221 gctattatac cttttgctaa agataaaaca ttcacacagt attttaaaca ataccaagtt
     8281 ccagcattat gtaacttagg aacagccttt ggtatgggac ttatcttaac aacatttatg
     8341 atttctcaag gtacagaata tgtacttcca gcattaattg gtaatttagg tgcaattata
     8401 ggtagtgtca taagtgttag aataatgttg acatttacta agaagtttta taaatacaat
     8461 ccggaagaag ataaagcaac aggaacatta gagaaaaagg atgaatttag agaaatcaga
     8521 gaaggaaatg tatttcaaag agctttagat gcaattcttg aaggtggtaa aatgggggta
     8581 gatatgggaa tggctataat tcctggagtc ctagtagtat gtacattggt gatgttatta
     8641 acttttggac catctacaga ccctgttaca ggtcaagaag tttatacagg agctgcatat
     8701 gaaggaataa aattacttcc agttattgga gataaacttg gatttatatt agaaccatta
     8761 tttggattta catcaccaga ggcaatagca ttccctataa cttcattggg agctgttggt
     8821 gcagcaatgt ctctagttcc agagtttata aagtcaggag ctataactcc taatgatata
     8881 gcagtattta cagctatggg tatgtgttgg agtggatatt taagtacaca tattggtatg
     8941 atggatgcat taaatgctag acagttggca ggtaaagcta ttctttctca tacaataggt
     9001 ggtttatgtg caggagcagc tgctcacttt atatttacac tcgtaggata aatgtaatca
     9061 gctaagataa aaaataatat acaaaaaaat ggttgttgat atgtaataag aatttacata
     9121 tcaacaacca tttttttctg actaaagata atttataata cataaacatt aagtggatat
     9181 tagagttaag ttatatttat gtaaaaagta acaaaaatct gtagaaattt atgacaaaat
     9241 ttagtacact aactatatgt tcttatttaa aatttaaggt tagaattagg aattacctag
     9301 tagagtaagc tttgattaat aaaagaatca taaaataaaa atattagtca taaagattat
     9361 aaataactta cttaaaataa agccatagaa cttacaaaaa ttaaaaaagc atataaatat
     9421 ggaggggtac aatggatgaa ttatttcata taggaattga tgtaggttct acaacggtga
     9481 aggttgtggt acttaataat agtaataata tagttcataa agaatataga agacattatt
     9541 ctgatgtaaa aaaatcagtt aaagaagtac taaatggtat atatgaaaaa ttgggagata
     9601 taaatactac cattataata actggttcag gtggaattgg aatatcaaaa aaattaggtg
     9661 taaaatttgt tcaggaagtt atatctagta caaaatcaat agagtatttt catcctgaaa
     9721 ctgatgttgt catagaactt gggggtgaag atgcaaagat aacatatcta agtggaggaa
     9781 tagaccaaag aatgaatgga atatgtgcag gtggcactgg agcattcata gaccagatgg
     9841 catcactttt aaagacagat gccagtggat taaatgaact tgctaaaggg tataatgtta
     9901 tatatcccat agcttcaaga tgtggtgttt ttgctaaaac agatattcaa cctcttataa
     9961 atgatggagc caatcagact gatatagcaa tgagtatttt caatgcagtt gtcgtacaaa
    10021 ctgtgagtgt cctttcttgt ggaagaaaaa tagaaggaaa tgttgcgttt ttgggtggtc
    10081 cattgtactt tttatctgaa cttagagaag catttaaaag agtattggat ttaaaagatg
    10141 aaaacataat cttcccagaa aatgcacaac tttatattgc tattggcgca ggattattat
    10201 cagtagatga agataatata caactaaaat ctctaataga aaaattggat agtataaaga
    10261 atattgaaga tggagaagta aatttattag aacctttatt taaagataaa aatgagtatg
    10321 aagaattttc aagaagacat gaaaaagaaa aaataaatta tgtggacata aattcaataa
    10381 aaagtaattg ttatttagga attgatgcag gctcaactac aactaaagca gcacttattg
    10441 atgaggatgg aagacttgta tattcttatt ataatagcaa tgaaggaaat ccattaaaaa
    10501 caaccattaa agttataaat gaagtatatg atatattacc aaaaaatatt aaaatattga
    10561 gttcaactgt aactggatat ggagaaggtc ttattaaaaa agctcttaaa atcgataacg
    10621 gagagataga gactatagca cattacaaag ctgctaaatt tttcaataaa gatgtagatt
    10681 ttatacttga tattggtggc caagatatga agtgcctaaa aataaaagat ggagtaattg
    10741 ataatataat attaaatgag gcttgttcat caggttgtgg ctcattttta gagacatttg
    10801 caagttcttt atctatgaca atagaagaat ttgcacatga aggtatttat tctcaaaatc
    10861 ctgtagattt aggttcaaga tgtactgtat ttatgaattc tagagtaaaa caagctcaaa
    10921 aagaaggtgc aagtgttgga gatatatcag cagggctatc ttattcagta ataaaaaatg
    10981 ctttatttaa agtaataaag attagagatt taaatgagat tggtaataat attgttgtac
    11041 aaggtggaac cttttataat gatttggtgc ttagaagctt tgaaaaacta attggaaaaa
    11101 atgtaattag acctaatatt tcaggaatta tgggagcttt tggttcagca cttatagcta
    11161 aagaaaaata tgaaagtgga tacaaaacat ccttgttatc taaggaagag ttgaataata
    11221 tcaagctaga agcaagtgta actagatgta aaggttgttc aaatcattgt cttcttacaa
    11281 taaataaatt ttctgataat gaaattttta tttcaggtaa tagatgtgag aagggtgaag
    11341 ctatttatgg tgataaaaaa ataggattag aaaagaaaaa accaataaat ttatttaagt
    11401 acaaatacaa tagaattttt agatacaaac cattggaaaa agaagaagca aaaaatgggg
    11461 aaatagggat accaagagta ttaaatatgt atgaagatta tccattttgg tttacttttt
    11521 tcaatgctat gggctttaga gttgtacttt cagataggtc atcaaaacag ttatatgaaa
    11581 ctggtataac tagtattgca tctgaaacag tttgttatcc aggcaaatta gttcatggac
    11641 atatagaaaa tttaattgca aaaggtataa aaaatatatt ttatccaagt gtaacaaatg
    11701 aaaataaaga ggattcaaat gcagataatt attataattg tcctgttgta atatcatatt
    11761 ctgaagtaat aaaaaacaat gtagagaata taagaaataa gaacataaat tatataaatc
    11821 catttataag tctaaatgat aaggaaaaat tgaagaaaag actctatgat gaattatcaa
    11881 gacatttttc tgatataaaa ataactaaag aagaagttaa tcatgcagtt gataaagcta
    11941 ctgatgaaca gaatttattt aaaatggaga tacaagcagc aggtgaaaga gcacttaaag
    12001 aaataaaaga aagaaatatg aaggggattg tgttatgtgg aagaccatat catatagacc
    12061 cagaaataaa tcatggaatg ccagagctta taaattcatt agatatggca gtgcttactg
    12121 aagactctat atgtcattta gccaatgtac aaagaccact tagagttgta gaccaatggg
    12181 tatatcattc aagattgtat aaagcagcta gttttgtaag agataaaaac tatttggaac
    12241 ttgttcaact taattcattt ggctgtggat tagatgcagt aactacagac caggttcaag
    12301 aaatacttaa tgaaaaatcg aagatatata ctattataaa gattgacgaa ggcaacaatt
    12361 taggagcagc taagataagg atgaggtcct taaaagcagc tatgtttgag agagaaagca
    12421 aaaatataga tgtaaaaaat ataaaggtaa acaggataca gtatgctaaa aataatcaga
    12481 taacgaacaa acatactata ttagcaccac aaatgtctcc aatacatttc caattccttg
    12541 aaaaagctgt taacttaagt ggttataata tagaaattct aaaagatact gatagtagtg
    12601 taattgaaga agggttaaga tatgtaaata atgatgcatg ttatccagct ataatagttg
    12661 taggtcagct aataacagca ttaaaatctg gcaaatatga tttaaataac acttctgtta
    12721 ctataactca gactggtgga ggttgtagag ctacaaatta tattggattc ttaagaaagg
    12781 ctatatacga tgctggattt aaagatgttc ctgtcattgc acttagtgta aatggaatag
    12841 aagatagtgg tataatggat aatatatcac taaaattaat caatagactc tttatgtctg
    12901 ttgtttatgg tgatttgctg atgaaagtac tatatagagt tagaccatat gaaaaagtta
    12961 ctggaagtgc aaatgctcta tatgaaaaat gggttgatat atgcaaatct tctcttgtta
    13021 aagcaaaaat ttctgtattt actagaaata taaaagatat agtaaaagaa tttgataatt
    13081 tagagatact agatatcaaa aaaccaaaag taggtttggt tggagaaata ttggtgaaat
    13141 tccatccaat tgcaaataat aatttagtag atatattaga gcgagaaggt gctgaagcag
    13201 tcgtgccaga ccttacaaat ttcttcttaa gttgtgcctt taatactata tacaaacaca
    13261 ctcatttaga aggaagtaga aagagtagaa tgataggaga ggcatttata tacataactg
    13321 gaatatatca aagagtatat aaaaaagcac ttgataagag tgagagattt tatgctccag
    13381 ccaatataaa aaatgttgct aaaagtacag aaccagtagt gtcattaggt aatcaaactg
    13441 gagaaggttg gcttttaaca ggtgaaatgg ttgaattatt aaatgaaggt gtagaaaaca
    13501 ttatttgtat gcaacctttt ggatgtttac ctaatcacat aattggtaag ggttctataa
    13561 aagaattaaa gaggttgtat aaaaatgcaa atataatacc tattgattat gacccttctg
    13621 caagtgaggt aaatcaactt aacaggataa aacttatgct ttctaaggct tttaaaaata
    13681 tataagattt atattttgat atgttaaaag ttaatgaatt taaaaatata ctctatatta
    13741 agtatctata aaaaagagtt atcctatttt ttagggtaac tctttattag ttaaaaaatt
    13801 atatgtatac tgatattaaa ggggggaagt aatatgagtt tagtatatga tgtttataaa
    13861 tcaaaattag gaaatttgta catattaagt gaagatggtt ttatagttag tatatacata
    13921 ggagatgaaa aatttaataa tttaaaaaat gaatgtatta aaaaaggaat tgatataaag
    13981 agaagtaagg aagatttaaa ggaagcaata agccagatag atgattactt taatggaaaa
    14041 agaaaagtct ttaatttaaa tattcgtatt gaagggactg aatttcaaaa atcagtttat
    14101 agggaaatgt taaaaatacc ttatggagaa acattgtgtt attcagatat agcaagaaat
    14161 ataaacaatc caaaatcagt aagagcaata ggacaagcaa gtaaaagtaa taaaattcca
    14221 ataataatac cttgtcatag agtagttggc aaaaaagata ttggtggata tatggggaat
    14281 cattctgatt tgaaagagat actattaaat ttagaaagac agagttaact tagaaagaaa
    14341 tgtttttatt gttaatattt gatataataa tagtaaaaag aagtattttt tacaaaaaca
    14401 atgaagaatt tattttaatt tataaaaatg agggggaatt ttaatgtgtg ataaagttat
    14461 agcatcaaaa tatcatgaga agggatttaa ttgtgcagaa tcagttataa aagcatataa
    14521 tgaggaattt aatacagata taccagtttg tttaggaagt ggactgggtt cagggtgtgg
    14581 ggttgctagt ttatgtggtg cagtaaatgc atcaaatata ataattggat atgtaaaagg
    14641 cagaaaccac gaagatgaaa gtactaaagc taaagtatat gctaaagatt taactacaac
    14701 agtaagaaaa gagtatggtt cagagttatg tatagaccta aaaaaagatt tagttgcttg
    14761 ta
//
LOCUS       DAGSHT010000078        14758 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGSHT010000078 DAGSHT010000000
VERSION     DAGSHT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.080"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..338
                     /locus_tag="MJB72_002084"
     CDS             <1..338
                     /locus_tag="MJB72_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HBY8263191.1"
                     /translation="EEYSKGILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGK
                     KKKIRVLDIVGAFSNIKGITNDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKK
                     MVKVKRDIR"
     gene            412..645
                     /locus_tag="MJB72_002085"
     CDS             412..645
                     /locus_tag="MJB72_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263192.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            801..1565
                     /locus_tag="MJB72_002086"
     CDS             801..1565
                     /locus_tag="MJB72_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8263193.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(1850..5029)
                     /locus_tag="MJB72_002087"
     CDS             complement(1850..5029)
                     /locus_tag="MJB72_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8263194.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPTNLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            5310..7487
                     /locus_tag="MJB72_002088"
     CDS             5310..7487
                     /locus_tag="MJB72_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY8263195.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            7501..7923
                     /locus_tag="MJB72_002089"
     CDS             7501..7923
                     /locus_tag="MJB72_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8263196.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            8097..8672
                     /locus_tag="MJB72_002090"
     CDS             8097..8672
                     /locus_tag="MJB72_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8263197.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(8797..9513)
                     /locus_tag="MJB72_002091"
     CDS             complement(8797..9513)
                     /locus_tag="MJB72_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY8263198.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            9678..10403
                     /locus_tag="MJB72_002092"
     CDS             9678..10403
                     /locus_tag="MJB72_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8263199.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            10463..12055
                     /gene="hcp"
                     /locus_tag="MJB72_002093"
                     /gene_synonym="priS"
     CDS             10463..12055
                     /gene="hcp"
                     /locus_tag="MJB72_002093"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBY8263200.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            12426..13331
                     /locus_tag="MJB72_002094"
     CDS             12426..13331
                     /locus_tag="MJB72_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY8263201.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            13455..14408
                     /gene="msrA"
                     /locus_tag="MJB72_002095"
     CDS             13455..14408
                     /gene="msrA"
                     /locus_tag="MJB72_002095"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBY8263202.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            14561..>14758
                     /locus_tag="MJB72_002096"
     CDS             14561..>14758
                     /locus_tag="MJB72_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263203.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFY"
BASE COUNT         5598 a         1674 c         2379 g         5107 t
ORIGIN      
        1 ttgaagaata ttcaaaagga atcctaaaaa aagtatcaaa gagaaaaaaa gttgataaaa
       61 attcttacaa aagtaaagat ataaaactaa atatagaaag tgaagttgtt aaattatatt
      121 taaatgctgg taagaagaag aaaataagag ttttagatat agttggtgca tttagtaata
      181 taaaaggtat aactaatgat gatataggtg taattgaagt tcaagattta tgttcatatg
      241 tagatatatt aaattataag ggagatttga tattaaaaaa atataaggag atacctataa
      301 agaaaaaaat ggttaaagtg aaaagagata tacgataatt tagtcaatat tgatgaattg
      361 ctttattatc gaaatgaagt tgcaaaaaac acaatagaag ttgttaagga tttgaaagct
      421 gaagatatga aacgaaaggt ctctccaatt gacttagaaa ggattttggt gactggagga
      481 gtaataaagc atgaagattc tatttggttg ttggactttt gggggaataa agatattgct
      541 ggaattttgc ttatgcctcc tactcgtcat gttatacttc atttgaatga ttgttgtaaa
      601 tggaaggaat attttcgtac aaaaaagaaa ttttacagaa gttaaattat aataaattag
      661 acaaacaata gataaattag caaggaatgt tgattttatc tacattctaa gttatactat
      721 ttttaagtgg ctaatggaat agtcatttta aatataagta ataaatatat acctataggg
      781 atattaaagg ggggaaatat atggcatatt ttaactataa cgacaaacag tgctactata
      841 atgaaattgg ggatggtaca ccattacttt ttttacatgg aaatacagcc tcttccaaga
      901 tgtttaatga tataatcgat ttttataaag atgaatataa agtaatatta attgattttg
      961 taggtcatgg aaaatctcaa atggtcgata aattttctgc tgacttatgg tttgatgagg
     1021 ctatgcaagt gatatgtttt ttagaagcaa tgaactataa aaaagtaaac ataattggta
     1081 gtagtggagg agctcttgtt gcattaaatg ttgcacttga gagacctgat ttagtgaata
     1141 aggtaattgc agatagtttt gaaggagaag ttcctctaga gtcatttgta caaaatgtga
     1201 aaatagagcg tgaagcatct aagcaagatg atggtgcaaa agctttttat atttataatc
     1261 aaggagaaaa ctgggaaaaa gttgtagata atgatacaga agctattttt gaacattata
     1321 aaactatagg aaaatttttt cataagcctc tagaaactat gcaaccagag gtgcttttaa
     1381 caggaagtag agaagatgag tttgtatctc taatatctag tgattttttt gaaaatactt
     1441 tttcattact tcttgaaaag attaaaaatg gtaaaatgta tttgtttgat aaaggtggtc
     1501 atcctgcaat tttatcgaat ggattagatt tttcaaatgt agcaaagaaa tttttagagg
     1561 aataatttaa ccaaaattta caataatgaa aactgtaaaa tggattgcaa aacttacaaa
     1621 agtgagtgta catatacttc attattatga tgaaatagta ttgttaaaac aaaatattct
     1681 aatagtcaag ttattacatt tgtatatgca atatgtatag aaagagatat cattagattg
     1741 taaagttggg attaaataac aatattaaat tttatttatt cagatatttt agcttaacta
     1801 tataaatgtt agaacaaatt tatatagtta agctgtagga ttaaataatt tagttaaata
     1861 agtttattat ctcctcatca gatagacttt ttaatacacc ttcattagaa agttctccat
     1921 ttataaattg atttataagc tcttttttac tttcttgtaa ttttattatt ttttcttcta
     1981 tagtcccttt agcaatcaat tttataactt gtactgaatt tttttgacca aatctatgag
     2041 ctctatcact agcttgattt tctactgatg gattccacca tgggtcaaag tgaattacca
     2101 tgtcagcaga tgtaagattt aaaccagttc cgcctgcttt taaagatatt aaaaaaactt
     2161 ttttatccat actattattg aactcatcta caagttcaag tctttctttg gcattagttt
     2221 taccatctat ataatggtat ttaattttat acttatcaag ttccttactt atgttcttaa
     2281 gcacagaagt aaattgtgaa aacaataata ttttgtggtt ttcgtttata ctatccctaa
     2341 gtatttctaa acatgtttca atttttgaac tttttttatt gtaatctttt actactatac
     2401 ttgggtctaa acaaagttgt cttagtttag ttaaataaga aaatataact attttatctt
     2461 tctttagatt tttatctttc attttatctt gtatatcttt tgaatataca ctatatatct
     2521 ttttctgctc tttattcaat tcaacaaaaa agtttttttc aatcttatct ggaagttctt
     2581 tcataacttg ttttttactt cttctaagaa taaaaggttt tatcaatttt ttcaggtttt
     2641 taacattatc ttctttattt ataaacaatt cctgaaactt tgctttacta tatagatagc
     2701 caggcattat aaaatcaaat atagaccata attctagtag attattttcc ataggtgtac
     2761 cagtgagtgc aaatttattt tctgcattta tactttttac agactcagtt actaaagcta
     2821 ctggattttt tatattttga gcttcgtcta atatacaata atcaaacttt atttcactat
     2881 atttttctaa atcatttcta agagtaccat atgttgtaag tattacatca aaattttcta
     2941 attccataaa acatttttct ctatctcttt tatttccatg tatcaataaa acttttatat
     3001 ctggagcaaa cttttcaaat tcatttttcc aattataaat gagtgatgtc ggtgtaacaa
     3061 taagactttt tttattagat aatgataata aaaatgcaat agtctgtatt gttttaccaa
     3121 gacccatttc atcagctaat ataccaccaa atttataata atctaaaact ttaaaccaat
     3181 tgaggccatt tatttgatag tcacgaaggt ttgcatttaa atttgtggga atttctaaat
     3241 ttatgctatc aaaatttcta aatttttcac atatactgtc tagttctttt ttaccatcta
     3301 taaactttaa atttttacta cgtattacat catttatata tagagcttta tttttatgaa
     3361 ttttattatt ttttgattta tccattatat ctagattttc aactaattca aaaaaatctt
     3421 ttgtttctct ttctgataaa tctataaagt ttccatcttt aagtttataa aaagtacgtt
     3481 tttcttgaaa agctttcaaa atatccttgt actcttttgg atttatatca gatatattaa
     3541 aattaaattc taggtaattc tggttagtct gattgattga agcttgaatt gatgaagcgt
     3601 tatatatttt tttcaattta aacttctcag aataatatat atctccaaat tcctttagag
     3661 attctaattg taaatctaaa aatgtaaata ggtcatggtc agttccttta aacacataat
     3721 gattattttc ctttgtaaat tgtgaagaag taagtttatt ttcaatagta gcttcctttt
     3781 gtatgttggg gagcacaaat tttttatctt catcatcata tatatacttt acatcacaac
     3841 atatgttgcc atcaatcata tcaaagtaga actcagattt aaaatctttt gttatattat
     3901 tttttatgtt atcatctaaa tgaacttctt gagatataga ttttaatcta ggaactagca
     3961 atcctaaaac atccttagaa tcctctttat caaattttaa ctctttagtc tttgataata
     4021 tttgatatat ttttgaataa tcatgagctt ttttatgtga taataaatag atattgccat
     4081 catataaaag tacatcattt tttttagtaa gtggaacagg tagtgaatct ttagaatata
     4141 agactatttt atcttctata tttttaaaat caaaccttac aggtagagta tcatttaata
     4201 tctttggatg atattcctct ttagaaaaaa taagggtaaa atccttgtac ttaatagcat
     4261 ttaaaaattg ttttaaagag cttgcattta tctccataaa tttttttgaa tgtgatgaag
     4321 aaaagttgta tgaaagattt gtcccatatt cttctataat atctactaac ttctcatctt
     4381 catcagcaaa ataatgagtg ttggggttgt aagtaaattt cttcccaaaa tctaattcat
     4441 atccattaag ttttgaatat gcaaattcag taatgttttt taaaatgtac attttatcaa
     4501 ttcctatttt aaattcagca attgctttat ttgaatccac atttatcaat acatctaaat
     4561 taaccctttc tcttttagta ttgtctaaca aagaaagtaa ttgcttgctt aaatctaagc
     4621 tggttttaga atttgtatta gaaagcttat ccttatctgc acctaatata tctattagaa
     4681 taggaggaac atctttttta ttttttattt gttttaagct ttttatcaca atagcaacta
     4741 tatgcttaca tataaaaata cttttgccct ttgattgaca gtcagcgcaa ctacaatcat
     4801 tttcaattat cttaaagtgc tttatatcta tagttattga attaaaataa aagctataat
     4861 tatcttcaga attgactcca ccacgaaatg tcaacaggtt attttgtagg tcatcaatat
     4921 ctatgtctat ataatcaact aattgattag tataatattt tacacctctt gaccatcttg
     4981 atggttctgt atttcttctt attagtttat atatcatatc tattttcata ttattcacct
     5041 tacctcactg tatatattat aatctaaatg cattcaattt acactctaaa tttaagacta
     5101 aaattaaggc agatatgaat agcttttatc tatcacaata tatgtgtaaa taacaattta
     5161 ctattagatt tttattgaat tatcttataa actatgaatg aaaaataatt atataaatag
     5221 attattaata aatataaaat cttattatta aaaatattct tagaaattac actatataaa
     5281 atataaagta tgatatttga ataaaaagaa tgtataataa gtataataat ttgtatttat
     5341 tattgggaaa ggaaaatgtt aatatggata gttatagaca acttaaagct aaaataccct
     5401 gtaaggaaac aggtatagaa ataaagagga ctttatgtga tatttgttct ccaggagtac
     5461 attgtggaat tgatgcatat gttaaagatg gtaaagtaat aaaaatagaa ggtacaaaag
     5521 aacatccaat gaataaaggg ttgttatgta ccaaaggtct ttgcaataga gaatatatct
     5581 atagagaaga tagaataaaa acacctctaa aaagaattgg aaaaagaggt gaaggaaaat
     5641 ttaaaccaat atcatgggat gaagcttata gagaaatagc agaaaaatta aatagagtaa
     5701 aatctgagta tggagcaaag catgtagctt tttttacggg ttatggaaag tggtatagac
     5761 cttttttaca aaggtttgca tcttcatttg gttcacctaa ttatggtaca gaatctagtt
     5821 catgttcaac atcagcacag atggctggaa atgttgctac tggaagaaac tttacaatgc
     5881 aagatatgga gaatgcaaaa acttatttag ggtggggatt taatccatac tactctaaat
     5941 ttttaaatag tgtgaatcta ataaaattaa aagaaaaagg tctaaaattt ataattgtag
     6001 accctagaat aacaccgaca actcaaaaac tagcagattt acatttaaga attaaacctg
     6061 gaactgatgg agctttagct cttggaatgg cgaatctatt tatacaaaag aatttaatag
     6121 atagagaata catagaaaag tatgtacatg gatttaatga atataaagaa tatgtaaaat
     6181 tatttgattt agatactgta agtagaatta caggagtttc taaagataat atagtaaaag
     6241 ctgtcgagat gttgtcacaa ggtccatttt gtataaatga aagtgcagct ccaatagttc
     6301 atcatcaaaa tggatttcaa aactatagag caatgatgtc tttatccgct ataacaggta
     6361 attatgatag aataggtgga aacataccaa aacaagatta ctttgctggt aagataggtg
     6421 gattccaaac actagaaaaa gagtttgtaa aagaagttct acctaaaaat ataaatgaaa
     6481 gaattggtgg agaaagattt cctctatgga atgaaatggt gactcaattt caagctatgg
     6541 atttacctag gcaaatagaa gaaaagacac catatgaaat aagagctttg ttttgtcatg
     6601 ggataaatgc tagaatgttt cctaattcag ggagactttt taaagcgttg aatagtgtag
     6661 atttttttgt gaatatagat ttatttatga cagatacatc aaaatatgca gatatacttt
     6721 tacctgcatg tacatcattt gagagagaag aatgtaagag ttatccagga gggtatatta
     6781 catataccaa aaaagttatt gataaattat acgattcaaa atcagatgta gaaatacttt
     6841 cagatttagc aaatgctatg aatatagatg atgatttatt aaaagcaggt tatgaagcaa
     6901 gtgtaagaca tatgtttaaa aatacatgtg tagatgtaga taaacttaaa gaaagtgatt
     6961 taccattaaa atgtaaagat tttaagccat atatagtagg ttcatataca agagaagggt
     7021 atgatactag cacttctagg tttgaattaa aatctacaat tattgaaaaa tataaagatt
     7081 ttgggctgga tgcactacca acatatagag gatttaatga taatatagat gatgaatata
     7141 ctcatttatt gacatcagga gcaagaattt caaatgcact acattcaaga cttcatgaca
     7201 tcaaactttc aagaatatta aaaaatgaag ctagttgtga tatttctagc ttagatgcaa
     7261 aagaattggg tgttatcagt ggagattata taaaaataac tactaagata ggaagtttgg
     7321 atgtaaaagt caatgttact gatagagtct tatataagaa tatacatatg tatcatggat
     7381 atagagaagc tgatataaat agtattattt gtgataattt tgacccttat agtggattcc
     7441 ctgcgtatag aagttgtaag tgtaagattg aaaaaatgaa tagataaatg gagagatata
     7501 atgaaaagtt tatttaaatt taataaagaa ttatgtacag catgtggagc atgtttgctt
     7561 gcatgtatag accaaaatga taataatata gacaatggtg aactttttag acatgtattt
     7621 acaatagagg aaggtgaaaa tatagaatat ttttcattag catgtataca ttgtaatgag
     7681 ccaaaatgtt taggtgtatg tcctaaaaat tgttttaaaa aagaagaagg attcgttgta
     7741 ttagacaatc aaaactgtat agggtgtaaa ctttgtgaaa aagcttgtga atatggagcc
     7801 ttaatttatg gaaatgatgg taaggcaaat aagtgtaatg gttgtattga gagatataag
     7861 tgtggtatgg attcaccttg tgtaatggta tgtcctacag gagcattaaa atttaataaa
     7921 taattgtatt aaattgctat actactttta aataattgtg ttaagaatta ttttttgcta
     7981 tacttttgta atataaaatg taaataaaac aaatacaaag tatataaaat atatttttag
     8041 aataaagtgt gctttagatt aaatttagaa aaataaatta gacaggagaa cttaatatgg
     8101 taaaagtatt agcctttatg ggcagtccaa gaaaaaaagg gaatgtagac acaatacttg
     8161 atgagataat aaaaggtgta aatgataata atgttgaagt taaaaaatat tatctaaatg
     8221 atatgaatat taaaggatgt caaggctgtc tatactgtag aaaagttcct acttgtgcat
     8281 ttaaagacga tatgattgac atatatgaag atataaaaag tgctgaatat ataataattg
     8341 gttcgccagt atatatatgt caggtatcag ctcaaacaaa gctactttta gatagattgt
     8401 atcctttaac agagataaat aagtctaaac atatacctag gtttggtcaa aaaaagttaa
     8461 tcatggtcta tactcaagca gctcctattt cgtttttatt taaaaaatat tttaagtata
     8521 ctgcaaaaca tttaaagggc atgggattaa atcactacaa aactatagtt gtaaccaaag
     8581 catttgaaag aaattccact aaaaaaaatt ttaaggtatt gaaaaaagcc tataaacttg
     8641 gaatgaaaat agccattgat atcaatggat agataaaagt ttaatttata gtatagctaa
     8701 aggagcatta attattaata ctcctttttt gtataaaggt ttctagaaaa taactaataa
     8761 aaaacaatat tatttgaata cagatattat acaagcctag atatctaact tgttttgaaa
     8821 ataaaagatt tcaaatttat ttttgttaaa agataacaag ccttcatctc gcatcttgcc
     8881 aagctctctt gacatagaac ttctgtcaac acataaaaaa tcagccattt catttcttga
     8941 aaagggaatt gtaaactggt ggctacctgt ttgttctatc tgttttgaaa aatacataag
     9001 caatttctcg cgagttgttt tacgagaaag taattctatt ttattgtgaa gtaaaatgtt
     9061 tttttcagct attaattgta gcatattttc aacaagccta ttatgaaata cacatgcaga
     9121 tgaacaaata ccagttacac ttttaaactt tataaataaa acttcgcaag aagatgttgt
     9181 ggttactgta acattacttt tatgtagtct agtacatgcg aatacctctc caaataaatc
     9241 tccaggcaag agttcagcta caatattcct attcccttcg atatcttcct ttattatatg
     9301 agcacttcct ttaagcataa ttccaataga agtaacttca cttccaacag agaatattat
     9361 actatctttt gggtaagtct tatctacaac acctaaacat tccaacattt cttcaagttc
     9421 atcactttta attccttcaa atagaggaat ttttttaagt aagtctatat gcttttctaa
     9481 aaattttttc aaataatcac ttccttgttg catatgcaac cgataatttg ttttcattgt
     9541 actatactaa gtttataaaa gcaaggggat ataaaaatcc tactacaaga aatttgaaat
     9601 aaagaaatat aggtattaaa aataaatata taagaaatca atactttaaa ttaaaaaaat
     9661 tttatgaggt gatataaatg atgagaaaaa taattaaaat tgatgaggaa aagtgtaatg
     9721 gttgtggact atgtgttgaa gcttgtcatg aagaagctat aggaatggta aatggaaaag
     9781 caaaactttt aagagatgac tattgtgatg ggctgggaga ttgtctgcca acttgtccaa
     9841 caaatgcgat ttcatttgag tatagagaag cagcagaata tgatgaagct gcagtaaaag
     9901 caaatatgga agcaaagaaa gcacaaaaga aaactttagc atgtggatgt ccaggttctc
     9961 agtctaagtc aataaataga gaggtttcaa atagtacgtc tataagcaat gatatagtag
    10021 agataaaagg ttctcaactt aatcaatggc cagtacaaat aaaattagta ccaactaatg
    10081 catcatattt aaagaatgca agtcttttaa tagcagcaga ctgtacagcg tatgcatatg
    10141 gaaattttca taataaattt atgaaaaata aggttacatt aatcggatgc cctaaacttg
    10201 atgaagttga ctatgctgaa aaacttacag agattttaaa agaaaatgat attaaaaata
    10261 tagtagtaac acgtatggaa gttccttgct gtggtggtat agtaaatgct gttaagacag
    10321 ctctacaaaa tagtggaaaa atgataccat ggcaaatagt tactatatct gtagatggaa
    10381 aaattgtaga tggagaaatt taattaagtg aattatagaa aaatataata aattttaata
    10441 aataaaaagg gaggaataaa aaatggaaaa aatgttttgt tttcaatgtg aacaaacagc
    10501 aggaggtaaa ggttgtacta taaatggtgt ctgtggtaaa aaggggggta cagctaactt
    10561 acaagacgaa cttacaggag tgttgattgg tctttcaaga gcaactattg gaaataagaa
    10621 tagaccaaca agtgaaacag ataaaattat gatagagggg ctattcacta ctataacgaa
    10681 tgtaagtttt gatgatgaag ctataaaaag acaaattgaa aaaactgagg tagaaaaaag
    10741 taaacttgta cctagatgtt cagattgttc aacaacttgt aatagaaatg ataactatga
    10801 tatgaaggat ttatggaatg ataatgaaga catacgttca ttaaaatcat taatcttatt
    10861 tggattaaga ggtatggctg cctatgcata tcatgcaatg gtacttggtt atacagacaa
    10921 agacgtaaat gaatttttct atgaaggatt aatagcagtt ggaggagact tgtctattga
    10981 cgaactttta ggtctagtta tgaaaactgg agaaataaac ttaaaatgta tggaattgct
    11041 tgatagagca aatactgaaa cttatggaac accagaacct acacaggtat ctatgaaaat
    11101 tgagaaaggg ccatttattg tagttacagg acatgacctt tatgatttac acaaattatt
    11161 agaacaaacg aaagataagg gtataaatat atatactcat ggtgagttat taccagcaca
    11221 tgcatatcca aaactaaaag aatataaaca tctaaaaggt aattttggta cagcatggca
    11281 aaaccagcaa aaagaatttg ataatattcc tgctccaata ttatttacaa caaactgttt
    11341 aatgcctgtt aaagatagtt atagagatag agtatttact acagaagtag ttgcttatcc
    11401 aggtatggtt catattgatg aagataaaga tttttctagt gtaatagaaa aagctcttga
    11461 acttggagga tatagagaag atgttcatat gactggtatt aatggaggag atacattaac
    11521 tactggtttt gcaagaggta cagtttcatc tgtacaagat aaagtagtag atgctgtaaa
    11581 gagtggagct ataagacact ttttattggt tggaggttgt gatggtgcaa aacctggaag
    11641 aaattattac actgaatttg taaaacaagc tcctaaagac actgttatat tgacacttgc
    11701 atgtggaaaa tatagattta atgatttaga ccttggggat ataggtggct tacctcgttt
    11761 aatggatatg ggacaatgta atgatgctta tagtgcaatt aaagtggcag tttcattggc
    11821 aaatgctttt gaatgtggag taaatgattt gcctcttagc atggtactgt catggtatga
    11881 gcagaaagct gtttgtatac ttcttacact acttcattta ggtatcaaaa atatctatat
    11941 aggaccttct ttaccagcat tcttatctaa gaatgtatta gatatattgg ttgataaatt
    12001 tgcattgaca cctatatcta ctcctgaaga agatatgaag aagattttag gataaataaa
    12061 tatatctata cttaaagata tttatagtta aaatattttt aagtatgcca caattaagat
    12121 aaggggttaa acttaaaatt tgattaaaaa atcaattttg agatgaccct ttatttattt
    12181 tttgtaataa ttgaaaaata aacctgcttt cagtatattc ttttaagaat ttttgtatta
    12241 aatttataat acttagatat ataatatctt atatatttta ttttaagtat taaaggtttg
    12301 aatgtaactt aaatattaaa agaggaacat tgtaatatgt aacttgcata aaatatatat
    12361 actatagtga aattattttg attaaaattt aaaatataag gtgttttatg tgggggtata
    12421 tgtatatgaa aaattctaat tttaatatat tggcatttga tggtggtgga ttaaaagggg
    12481 cattgagtat atccatactt gagaggatag taaaagagta tccaaatcta ttaaacaata
    12541 taaatatgtt tggaggtaca tctacaggga gtctaattgc actagggctt gcgtatggtg
    12601 taagccctaa agagataaaa gaactttact caattgaaaa ttcaaagtat atttttaata
    12661 aaagttatgc agaaatactc agaccaaagt atgaaaacaa aaacttaaaa gaagttttat
    12721 taagtatatt tccagaggaa ctagaattaa aagatttaaa taaattagta atgataccaa
    12781 gtttttatat aggaaatgag gaaaatgcat ggaaaccagt attttataac aatatgccaa
    12841 attcttttac aaagactagc aaagttgtag atgtagcaat ggcaagtagt gctgctccgg
    12901 tattttttcc aacatataat cgtcatgttg atggaggaat aatagcaact gacccgagtt
    12961 tagcgtgtat aattcatgcc attgacagtg gatttagact taagaataca agactttttt
    13021 caatagggac tggatatgta tataacagta taaaggcaga tactaccgaa tggggagcta
    13081 ttgactggat aattaataaa gaacccgatt taccaattat atctattacc ttagaaggaa
    13141 attctcaaat gagtcagtta ttttctcaaa aacttcttgg agataattac tatagattaa
    13201 atcctaagat ggaaaaagat gtagctatgg atgattgtga tgcattagaa tatctaatgt
    13261 ctttaggaga aaattgtgat ataaaagatg cttttggatg gataaagaaa aagagctttt
    13321 atagaaatta gattcaattt gcaattgtat ttttacacat gatttcattg agtaggtaaa
    13381 aataataaaa tgcagattat taagttactt cttagggaat aaataaagta taatttattt
    13441 aaggagtgat gaaaatgata aaacttgcta cttttgcagg tggatgtttt tggtgtatgg
    13501 taaaaccttt tgataagtat gatggagtta ttaaagtagt ttctggatat acaggtggtc
    13561 atgttgaaaa ccctacatat caagaggtat gctcaggaac tactggtcat ttagaagcaa
    13621 ttcaaataac atatgatgat gatataataa gttatgatga acttctaaat atcttctgga
    13681 aacagattaa tcctacagat gatggtggac aatttggtga taggggaact caatataaga
    13741 ctgctatatt ttatcatgat gaagaacaaa aaaatatagc tttagagtca agagaacatc
    13801 tggaaaatag taaaatattt gatagtaaaa tagtgactga tataattcca gcaactaaat
    13861 tctacgaagc agaagaaaat catcaggatt attataagaa aagaccatta aactacaata
    13921 tgtattataa agggtctgga agatataatt ttgtaaaaag ccattgggat ggaaacaata
    13981 taaataggga agagttaaaa aagactctaa ctcctataca atttgaagtc actcaaaatg
    14041 atatgacaga agtacctttt gaaaatgaat attatgacaa taacaaagag ggaatttatg
    14101 ttgatattgt aagtggagaa gtgttatttt cttcaaaaga taaatttgat tctggatgtg
    14161 ggtggccaag tttttcaaag ccagttatag ataaatctat cattgagaga agtgatttct
    14221 cacaaggtat gtacagagtt gaagtaagaa gtgctaaagg aaattctcat ttaggtcatg
    14281 tatttgatga tggtccaaaa gaacttggag gtttaagata ttgtataaat tctgcttctc
    14341 ttaaatttat accaagagaa aatatggaag ctttaggata tggagaatat ttaaaacttt
    14401 ttgagtaatt aggaatattt attataaact tatctaaaag gtgaaaatat aaaaaaatat
    14461 tttcaccttt tgtttttttg tcaataatat gataaattat taattgtaat cttatttata
    14521 ccaatagatt aagaatttaa ttttgaaaga gagaaaacaa atgaatttaa aagactttaa
    14581 tggaaagaga ttaaaatcag ctagaatttt tagagcaaag acgatagaac agttgtctaa
    14641 agaaactaaa ataaataaaa aagatttaaa agcttttgag gaaaataagt atgtaccaaa
    14701 tatagaaaat acattaaaat tatctaatat attaaatttt ccaaaagagt atttttat
//
LOCUS       DAGSHT010000079        14125 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGSHT010000079 DAGSHT010000000
VERSION     DAGSHT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.081"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            54..785
                     /locus_tag="MJB72_002097"
     CDS             54..785
                     /locus_tag="MJB72_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBY8263204.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            844..1755
                     /locus_tag="MJB72_002098"
     CDS             844..1755
                     /locus_tag="MJB72_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8263205.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            1806..2618
                     /locus_tag="MJB72_002099"
     CDS             1806..2618
                     /locus_tag="MJB72_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBY8263206.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            2727..3419
                     /locus_tag="MJB72_002100"
     CDS             2727..3419
                     /locus_tag="MJB72_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8263207.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3506..4447
                     /locus_tag="MJB72_002101"
     CDS             3506..4447
                     /locus_tag="MJB72_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263208.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4484..5641
                     /locus_tag="MJB72_002102"
     CDS             4484..5641
                     /locus_tag="MJB72_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY8263209.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEKDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            5793..6353
                     /locus_tag="MJB72_002103"
     CDS             5793..6353
                     /locus_tag="MJB72_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY8263210.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6381..6872
                     /locus_tag="MJB72_002104"
     CDS             6381..6872
                     /locus_tag="MJB72_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263211.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7101..7652
                     /locus_tag="MJB72_002105"
     CDS             7101..7652
                     /locus_tag="MJB72_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8263212.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            7738..8670
                     /locus_tag="MJB72_002106"
     CDS             7738..8670
                     /locus_tag="MJB72_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBY8263213.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNGHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            8725..9588
                     /locus_tag="MJB72_002107"
     CDS             8725..9588
                     /locus_tag="MJB72_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY8263214.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            9835..11697
                     /locus_tag="MJB72_002108"
     CDS             9835..11697
                     /locus_tag="MJB72_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8263215.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            11700..13166
                     /locus_tag="MJB72_002109"
     CDS             11700..13166
                     /locus_tag="MJB72_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY8263216.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            13190..14026
                     /locus_tag="MJB72_002110"
     CDS             13190..14026
                     /locus_tag="MJB72_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8263217.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         5589 a         1358 c         2541 g         4637 t
ORIGIN      
        1 tgttataata attgtttgta ttaacttaat aattataaca aggagtaata ataatgacta
       61 gtataaaaaa taaagatatt ttaaattgta tagattattc cattaaaaat aagctctttg
      121 acaaactaaa tgatgtatat aagtccttgc cagtaggaaa ttgttctgga tgtggaaatt
      181 gttgtatgga gtcagtaggt ataaatttaa ttgagttttt gaatatattt tgttatcttg
      241 aagatagagt tgatttaaaa aaaagctgta taagtaaaat attagattat tattttgagg
      301 agtatacaag aaagaaccca tgtccattta aagatactaa taatagatgt ttaatatatg
      361 aagttagacc attaaattgt aggatgtttg gacattggaa aaaagaggat tataataaaa
      421 atttagataa tgttattgag aaaaataata actatagaga gttaatgaaa aagcaatatg
      481 gatttgaaat aaatgatgaa gtagtaaatt acaggataga ttattgtgat agatttattc
      541 caaataaaga ttatttaagt aaatctaaga gattaagctt ttttgatgag cttatgatat
      601 tagactcaaa tatttattca agtggaaata tagatataga ctttagggat agaggtatag
      661 ttgaatattt tatagagtca ttactttata gaaatttagc atataatgtg aaaataagga
      721 tatctaaaga atacaaaata agaaaaagaa ctatagatag aataaaaagg attgttcttg
      781 tatagtttta tattagtaag taataaattg tatcataaat attgtcaaaa tgaggtgaag
      841 aaggtgaatc ttacaaaagt aaaaggaaat acctttttta taaaaggtgg cacgaataca
      901 ggaatatatg tatttgaaga taacacagca cttatgatag acccagggtt atgtggttca
      961 agacctagga gagtaaataa agtattagat gaaaataata tacaggttag atatattata
     1021 aatactcatg gtcatgatga ccactatgga gcttgtaatc aatttaaaga atattataaa
     1081 gatgtgtgta taatgtcatc tgaatacgat aaattatata tagaaaatcc agaattgttt
     1141 tcaaagtata ttgttggggg aaagagcaat gtatttatgg ataatatctt aaaaaataaa
     1201 atgttggatg atataaaaat agataaatct atagaagctg gaaaatttga attaaacaat
     1261 gaaatgtttg atataattga atttcagggt catacacctg gtagtatagc tgttttgacg
     1321 aaggataatg tgatttttgt tggcgattta ttgataggag atgaaatttt atcaaaatac
     1381 gatttcttgt ttctatatga tatacaagaa caaataaatt cattagaaaa attaaaaaat
     1441 atagattttg agtacttagt tttagggcat ggcaagcaag ttatatcgaa agaagattct
     1501 tataaattaa tagaaaaaca tttgaatgct ttaaaaaaat atttatatca aataagagag
     1561 ttgcttgtag aaccgaaatc tttagaaatt ttattaaaaa acattataaa taacaataat
     1621 ttaagcaata attataaaga gtatcatttt tttaaatctt cgttagtatc tgtcataagt
     1681 tacttagttg atttagaaga aatagggtat atattggaaa atggagaatt gctttactat
     1741 acaaaaacaa aataaattat gataaaataa agaaggtcat aaaaaacaaa taaagggtga
     1801 gcaatatgga atacgaaaaa tggaatgaga tactagcacc ttatgaacat gcagtagagg
     1861 aattgaaaat aaaatttaaa aatataagaa aagaatattt agcaaaaggt gaatattctc
     1921 ccatagagtt tgtcacaggt agaacaaaaa aaatatcttc cataatatct aagttaaaaa
     1981 ggataaatgc aaaagatata gaaactgaaa ttgatgatat tgctggtata agaataatgt
     2041 gccagtttgt tgaggatata tatgctatag ttgatttgat aaaagtaaga aatgatatga
     2101 ctataatagg agaaaaagat tatattacaa attacaaaga tagtggttat agaagctatc
     2161 atgttataat aaaataccct ataaactcca ttgcgggttc aaaggaaatt atatgtgaaa
     2221 tacaaataag aactcttgca atgaatttct gggctacaat agaacattca ttaaaatata
     2281 aatatgacca ttacatacct gagactcttg ctgaaaggct tagaagagca tctgatgctg
     2341 cctttctttt agaccaagaa atgagtgaaa taagagagga cataatgaaa gctcaggcta
     2401 tgtaccaaat gaagtctata gctattagag atactctaaa tagaatacaa gagttatatg
     2461 acttgggtga tacacataaa gctatgcagt accaaagaaa acttgataga actgaaacag
     2521 ataaaaatat aactgaaatt ttggaattaa aacaagaaat agatatatta ttagagcaat
     2581 ataaggatac taaggtagat gataaagtag ttcagtaaaa actacttgta gaaaatttgc
     2641 taaatattaa tggggctgcc tcttaatgga aaataatttc taaaaagaga cagcctattt
     2701 agtgtaatta agaaaggaat gatattatga gtttgtatgc tataggtgat ttacattttt
     2761 caacatcagt caataagcca atgaatatct ttggaagtaa ttgggatggt catgaaaaga
     2821 aaattataga taattggaaa gaagttgtta aggaagaaga tatggtctta gtactaggag
     2881 atacatcttg gggaattaat ttatccgaag ctaaaaaaga tttagatata ataagcaaac
     2941 ttccaggtca gaaaatactt ataaagggaa atcatgatta ctggtggaca accgttacaa
     3001 gtttaaataa gctatatgaa gacatgcgtt ttattcagac aaatttttat gaatataaag
     3061 attatgcaat ttgtggaggt aggggctgga tatgtcctaa tgatgttaaa tttgatgaga
     3121 ctgatgaaaa agtttataaa agagaagaac atagacttag actatcatta gaatctgctc
     3181 gtaaaagtgg tcactcaaaa ataattgtga ttactcatta tcctcctacg aatgataaat
     3241 tggaagaatc tttatttaca aagttatttg aagaatataa tgttgaaaag gtgatttatg
     3301 gtcacttgca tggtaaagaa tcttttaaaa tgggattgaa ggggattcga aatggagtag
     3361 aatatacatt agcatcttgt gactatacag aatttaattt aataaaagtt cacgattaaa
     3421 aaacataaat tggtaaatac tataagatac atgattaaaa ataaatattt gagggatttc
     3481 ggttttgaga aaggagacac gacttatgag gtggatatta gtttttatat ttttatatct
     3541 tattccttta gtagtgatat tcagaaatta caagaatttt aagaggtctt gtatctacag
     3601 tagtatttat gtggtattag ctacaacaat aatgataagt aatatttata tgagtggact
     3661 taataaaata aaagagtcta tgtattatca caattatatt tctgatgaga catatgatga
     3721 aaagtatgta tcaaatttta aggaaaaaga aagtactgat ttaaaacagg ataataaaaa
     3781 gaatgaagaa atatcaaata ataaaaattt aaacaacaaa caagatagaa atatacaaaa
     3841 taaagaaagt aatgaagaag gaagtaatga tataaaagaa aagaataatt ctgatagtaa
     3901 tgtttcagtg aataataaat cagaaaaaca agaagataat acaaaagaaa ataataaata
     3961 tacaagtaaa gaaaaaaata gtgaagatat aaaaaaaata gatatggaaa gtataggaat
     4021 ttttaagaaa gaaatttata atatagaaag agcagcttta gttccaatga gagaatgtat
     4081 accttacact aaggatatag caaaaaatat aactaaatta tctagtataa aaaaagatgt
     4141 tacaaatgcc agaaacaagt gtaaagaagt tgtaaaagaa tatgaaaata tgcagattcc
     4201 agaactttct aaggagaagt atacagaaac attgagtaat gctagagatg acgtaaagaa
     4261 agcatatgag cttagagaaa aatctatgga aagtgctttg aaattaataa atactaaaag
     4321 ccctaaatat atagggaaga taacagaata tcttgattta tcagataagc agatagaaag
     4381 ttttaaaaat agattgaacg atttaaaaga agaaataaat aattcagaaa caaataatat
     4441 taattaataa gatttaaaga tataagttaa aagggagatt atgatgaata aacagagtat
     4501 aggaaaacta aatatgtttt ttatggggat tactcaaaga aaccttgata atagggattt
     4561 ttttattgag ttagaggtga tttttaaatc tggaagaaaa gagtttaaag gaattgcaat
     4621 agagaaagat aataaatata aatttaattt taaaggaaaa tcagatttat atacgtttaa
     4681 tgagttgctt aagaatattt ctaaagaggc agaaaattat gatggattag tatttaaata
     4741 tgttgagcga ggaactattg ttgtaattga aggagacaat aagaaggtaa atgtaaaata
     4801 tttagataat aaggaagaag tacctaaaat agatgaattt acggcatctc aaataaaaaa
     4861 cagagattat tatgtaaaag taggacaagc aaatgcactt ttaaaagaaa ttggagtact
     4921 tacaaaagat ggtaagataa aaaatgataa aataagaaaa tacaatcaga tagaccattt
     4981 tgttgaattg atagatagta ttttaaaaga aataaaggat aaagactgta taactatctt
     5041 ggattgtgct tgtggaaaat cttacttaag ctttgtctta aatttttata taaaagaagt
     5101 attgaagaaa aactgttatt ttataggaat agattattca gatgtagtta tagaagcatc
     5161 aaaaaatatg gctaaaaact tgggttataa aaatatgtca tttataaaag aggatttgac
     5221 taattataca cctaataggg atgttgacct tgtaataagt ctacatgctt gtgatacagc
     5281 tacagatatg gcaataggtc taggaattag ggcaaaatct gaagctatag tagtagtacc
     5341 atgttgtcat aaggagttat tagggcaata tagatatgaa gctatggaac caatattaaa
     5401 acatggtgta tttaaagcta gatttgcaga tttgataact gatggtttaa gaacattgtt
     5461 attggagggc aatggatatg atacttctgt agtagaatac atatctccac ttgatacacc
     5521 aaaaaattta atgataagag cgataaaaac taaaacgaat aatgacaaag cattaaaaga
     5581 atataaagaa ctgaaatcac aatttggagt ggaaccaacc ttagaaaaac ttatatatta
     5641 gttaaaaatg taaaatttac gaaaagcttg cttaaatatg caagcttttt ggtattatat
     5701 aggtatagtc atatccagag ttttgattta taattaaagc tgtgctataa tatttgatta
     5761 atcaaatcaa taaatgttac atggaggtaa aaatgaacaa attgaatata tgtattgata
     5821 tagatggaac aataacaagc ccttatcatt ttcttccata cctaaatgat atctttaata
     5881 aaaatataac tgaagcagag tgtataacac ataattggga agaattgtat ggttcgggaa
     5941 taagagatgt gtatgcggag tttaataata agtatataca ctcatatgat gaagcaaaga
     6001 ttgtagaggg tgctacagag gtaattagca cattatctaa aggacataat ttatcttttg
     6061 taacggctag acatgagtgt ttaactgatg taactaaaaa ttggctatcg agacaaggat
     6121 tttcagatat agatgtttac ttattaggaa gtgattataa agttgaaaag gcaagagaac
     6181 tgagttgtga catatttata gaagataatc ctttaaactc agtgcaacta gctgatgatg
     6241 gtgttaaagt aatacttctg gatactaact ataataaaga tgttaaacat caaaatatag
     6301 ttagagttag taattggata gatattgaaa agattataaa acaaggtatt taaagatttg
     6361 tagattgaaa gggggagcat atgatagatt taaaaattga aaatagaaaa ggaaaaatcc
     6421 atgtaaagag cagtgaaata aaagacattt tagaattacg tccagacttt gaatatgtac
     6481 aagatatatc aaatactata aatcaagaaa atatattggt atttgattgc cagttgacag
     6541 gaaattcttt tgatatggat gatttagata tagaagaaat attggaagag ttaggtgaag
     6601 aaatagatga gtcttatttc agtgttttat ttgaagatgt aagagcgtat ttaaaagatg
     6661 ctactgatga gatagaagca gacttacagg ataattactt atttgataat ttgagatgtt
     6721 attttgatat atacaatata aatcaagaat ttacagattt taagtttgta tttttagttt
     6781 cttttaaaga tataaaaatt tcttcattag caaacctagc taagattgtt tctaagagac
     6841 agcttatagg tgcatctaag ttttatagtt aaaatgaaat attattataa taaaagctag
     6901 taaccttgta aaaatatagg ttactagttt tttatttatt cattttcaca aaatttcaga
     6961 aaactattta cattctaaaa aaagtaatgt atattaaaca taatgtacat tataatggat
     7021 aaaaagtttc tattaaatta ctaagtttat ttaataatca aaataattta tcttatatta
     7081 gtatgtatta gggggtattt atgcaaagtt taaatgaaat aattgctgaa aatttaaaaa
     7141 agataaggaa agaaaaacat cttagtttag acaagattgc acaacttagt ggtgtaagta
     7201 agagtatgct atctcaaatt gaaaagtgtg aggtaaatcc tactatatct actttgaaga
     7261 aaataactaa tggactaaaa atctcattta catcattgat ggagaggcag gaaagtgaca
     7321 tagaattgat tcaaaaaagt gatatagacc attttattga agataatgga aagtatataa
     7381 gttatccaat atttccattt gactccaaaa gaagatttga gatatttatg attgaaatag
     7441 aagaaggtgg aaatttagat tcaaatgctg aacttccagg aacacaagag tttataactg
     7501 tttttagtgg cgaagtaacc attaaaataa atggagaaga ttacattgta tctagtggaa
     7561 attctataag atttaaagct gatgtatcac atatatatag aaactcaggc aaaggtatag
     7621 ctaaaatgag tatggtagta tattatatat aaataattta atggaattag agtgatattt
     7681 agatgatttt aggtatgaag atatattata tttaagttat aggagtgtga tttatggatg
     7741 aataataaaa aagcagtttt tttcttttgt agtgatttaa agaaagaccc agtagccagt
     7801 aatgtgataa agtgttgtga agaaataatg gatttaaatc aaactgatat tgttattgat
     7861 gataattttg tattagaatt taaggataaa aacaataact tatttcattt tgtaaaaaca
     7921 aaagatgtaa taagtcataa ttataaatat tatcttccaa ttttaaatgg acattttaga
     7981 gattatgatt ttgcaggagt tgtaaattgg catgagggag aaaatgctcc agaacatatc
     8041 ctaacagctc atacaattgg agatgttcca acaggagaat ttggaaattc aaatccaaga
     8101 tactttaaaa atctaatcaa tgctattgag gatataaaaa atgaaaatct acttgatgaa
     8161 tttactacat taacagaagc aactcactgg tctggaacaa catatggtga agaatctaaa
     8221 ttaattacag aatattctgt acctatgttg gatattgaga taggaagtag tagtgatagt
     8281 tttaataata gtatagcaat acaagtattg gcaaaatcat taattagagt atttgattgt
     8341 gatgaacctt tgaagacttt gttatgtgtt ggaggggtac attttgagaa atctttctca
     8401 gatattataa aaaataaaga atataacata tcaattggac atgtattgcc aaatcaatgg
     8461 attgtaagtg gaatgtatga tgatgaatct ggatttgaga aactagaaaa gtgtataaat
     8521 agtattgaag gaggaattga ttgtatagtt tttcatgata aattaaaagg tacctataaa
     8581 gagcaatgcc gtaaactagg tgaaaaactt aatgtacctg tatttaaaca taaaatatta
     8641 aaaaatccaa aagacttacc tatatggtag tcttatacag ttaatataac aataagtcgg
     8701 attaattttt aagcgaggtc aaaaatgata ttatcttatt ttgcattatt aattagagta
     8761 gtgctattag cttttgaaag agtagttgta agattattag gtgatgaaga aggagatatt
     8821 tataaaaata tagcttcatc atttctattt ttctttatag ggggagtatg tttacttcca
     8881 tttagcataa tggatagagt tagtgattgg tcgtttttaa taccatgtta tataagtagt
     8941 ttagtctatt caattggttc agtagcgtat gtaacgtctt tagcaactgg ggaagtttca
     9001 cttgttactc caataaatag tttaaattca ttgtttttac tgcttttatc agttatattt
     9061 ttaggtgaag gtttaagttt agcaaaagtt gcaggaatca taattatgat tggtggagtg
     9121 tttatattaa aaaaagtaag ctctttttta aagaatgtct ctgtaatttt taacaattta
     9181 ccatgtagat taatgttttt atacatattt ttgcagtcag taggaagagt attggataag
     9241 gctttttttg tacaagtatc tcctatatta tattcaacta tactgtattt tttcgtaggt
     9301 ttaaatttat ttatcttttt agcatttaaa aagaaacaaa aaataatttg tgagattttt
     9361 actaataaaa agggactttc aattttaagt ggtgctatta atggatattc ttatttagca
     9421 ttattaattg ctttaaataa tctagaactt agtatagcag agccattttc tcaagtatct
     9481 atgataataa ctttaatact tgctcatttt atattcaaag aaaatataaa agaaaaaata
     9541 ccaggttcaa ttttgatact aattggtggg tggctactgt tactttaatt gacataaaaa
     9601 atgaattata aaggtgctat tagttagtat cagaaaattt aaaaaagtat tgacaattct
     9661 tttaggcaaa tgttaatata ataatacaaa atagtataaa tagaagacat gttactggta
     9721 atgcaggcag gatcgaatat attgctttga tttttcaagg tgtttatata ggttctgcct
     9781 tttttgcatc ttgaatggcc attcataaaa aattatggag aaggagatta caagatgaat
     9841 tataaaaaaa ctgccattga cattattgaa caagttggtg gttcaaaaaa tattttagct
     9901 ttagaacact gttcaactag actacgcttt aagttgattg atagaagtaa agtcaatcag
     9961 gatgctttaa aaaaagtaca aggtgttatg agtatagtta atggggcaca atttcaagtt
    10021 gtcattggaa atagtgttgt tgatgtgtat gatgaaattt taaaagtttg ccctttagat
    10081 acaaatgata gtactgtaaa aactgaagaa aaaataaact taggaacaag atttatagaa
    10141 tttattatta atatttttca accactgatt tttgctattg caggagctgg tattttaaaa
    10201 tcactgttga tgttattagc aatgtttaat attttaaaat cagattctac tatatataca
    10261 ttattagttg ctatttcaga tgcaacattc tatttcctac ctcttatggt agctgtaact
    10321 actgccaatg tattgaaatg taatcgttta gtagctattg ccgctgtagg atacttattg
    10381 cttccagcaa caacaactgc tttaagtgaa ggtgtcgaac tatttggatt tacaattcca
    10441 aacattgcct ataatgcaca ggttttccca gctattttat gtgtgtcttt cttggcaaca
    10501 atggagaaaa tttttaacaa gtattcacca aaaccaattc gaactttctt tgttcctatg
    10561 atgtctttgg caattactgt tccaattact ctaactattt taggtccttt aggatataac
    10621 gttggtacaa tttttacaac cattatttta ttcttgtata acaaaatggg attcttagtt
    10681 gtaggattac ttgctgcttt tcttccattt atggttgcaa caggaatgca caaagcacta
    10741 ataccttatt caattaatac aattggaaag ttgggttatg aagctttata tatgacatct
    10801 tcattagctc acaatatttc tgaaagtggt gcttgttttg cagtagcctt gcgaacaaaa
    10861 gatgaaacat taaaacaaac agcattatct gcaggaattt ctgccttgat gggaattaca
    10921 gagccagctt tatatggtat tactttacaa cataaaagag caatgttagg tgttgtattc
    10981 tcaagtgcaa tttctggtac atttttagga ttatttgcag taaaaggatt tgtaattgtt
    11041 ggaccagggc ttgccagtat gagtatgttt attgacccaa acaatggaaa caatttgatt
    11101 tttgcaagta ttggttttgt tattgcaata ttaggctcgt ttattattac actgattatt
    11161 tggaaggaag atacaaatac agtagagtca tcaattggag atgttgttga agaagagcta
    11221 gaagaagaaa aagaagtaaa tatcttagta actccagtag aaggaaaagt aatagattta
    11281 agtaaggtta atgatgaact atttgcaagt aagactttgg gagatggagt tgcaattata
    11341 ccgaccaatg gaaatttata cgcaccttgt gattcagaag ttgtgatgtt atttgaaaca
    11401 aaacatgcaa ttggtttaag aacaaaaaat ggggctgaga ttttgattca tattggaata
    11461 aatactgtaa gtatgaatgg agatggattt aaaacatttg ttaagacagg cgataatgta
    11521 aaagaaggag atttattaat tcaatttgat ttagacaaaa tctcacatgc taatttagat
    11581 tcaactgtta tgattgtaaa caataatgga gcagattatg cttataaagt gttaaaccag
    11641 tcttatggca atgtaaaaaa aggaagtatc ttgtttgatg taaagggagg aatataaata
    11701 tgagaaagtt tccagaagga tttctttggg gaggagctac agctgctaat caatttgaag
    11761 gtggttggaa tttaggtggg aaagggtggt ctgtatcaga tgtagcaaag gcacactttg
    11821 atgcagatgt gaaagattat aaatctaata atgaaatcac aacaaaagat atagaagaag
    11881 gattagcaca tcctgaagat gaagtaaatt atccaaaacg tcatggtagt gatttttatc
    11941 atcactataa agaagatatt gctctaatgg cagaaatggg atttaagaca tatcgaatga
    12001 gtattgcttg gtctcgtatt tttccaaatg gagatgataa agagccaaat gaagaaggtt
    12061 tacagtttta tgatgatgta tttgatgagt tgataagtta tggaattgaa ccacttgtta
    12121 ctatgtcaca ttatgaacca ccattaaata tcgttttaaa ctatgatgga tggtattcac
    12181 gacaagttat caatatgttt gttaggtatg tagaaactat ctgtgaaaga tataaaaaca
    12241 aagtgaaata ttggcttaca tttaatgaag ttgattctat gattcgtcat ccgtacacta
    12301 ctggtggttt agttcgagac cgttttaagg ataaaaactt tgaggaagtt atttttcaag
    12361 ctatgcatca tcaatttgta gctagtgccc ttgcaacaaa aatttgccat gagattattc
    12421 caaattcaaa agtagggtgt atgttaacaa aattaacgta ttatccatat acatgtagac
    12481 cagaagatgt tttggcaact caacaaaaaa tgagaagtat ttatgcatat agtgatacgc
    12541 aggtttttgg tgagtatcca gtatatttac tgtcgtattt taaaaataat aacattcaaa
    12601 tagtaaaaga agaacatgat gatgaaatta tgaaaaagta tccagtagat tttatttcat
    12661 tctcttacta tatgtcttct tgtgaggcag ctgatacaac tggattagat attacacctg
    12721 ggaatacgtt attagctgta aaaaatcctt atttagaaat gagtgaatgg ggttggcaaa
    12781 ttgattcaat aggtctacgt atatctttga ttgaattata tgatagatac agaaagcctt
    12841 tatttatagt tgaaaatgga ttgggagcta aagatatttt aactgaagat aaaaaagttc
    12901 atgaccaata tagaattaat tatttaaaag aacactttaa atgtatgtta gatgcgatta
    12961 tcgaagatgg cgttgaactt tggggatata cttcatgggg ttgtattgat ttggtcagtg
    13021 aatcaacgaa acaaatgtca aaacgttatg gatatattta tgtggatgct gatgactatg
    13081 gaaaggggac atataatcga tataagaaag atagttttta ttggtataaa aaggtaattg
    13141 aaaataatag tatagatttt aaatgaccaa tagaaggtga gcaaataaaa tgaactacat
    13201 aataaagaaa gttttgaact cgagcgttgt tttagttcat gatgcaaagt ataatgaatt
    13261 tattttgcta gggaaaggaa ttggatatgg taaaaaaaca ggagaatata tttgtggttc
    13321 caatgataat caaatgtttg tccctgttga aaatacaaaa tcaaaacaat ttttagaatt
    13381 aatggatgat attcctgtcg acattttaaa gataacacag gaaataatag tagaggcaga
    13441 aaagttattg aactcatctt ttaataaaaa cttatactta atattagcag accattttaa
    13501 ctttgcaatt gaaaggatgc gtaaaggaat aaaaattaca aatcgagtat tttgggaaat
    13561 aaaaaattat tatccaaatg agtttaaggt tggaatggtt gcaatttata tggtagagga
    13621 gagattgggc attcaattac cagaggaaga agcagctaac attgctttcc attttgcaaa
    13681 tgctatggct tcagatggaa attcatatga tacgattaag tatgccaaag taattggaga
    13741 aataattaat atttttgttt tttctttgaa tagaacctta gataaaaaga gtatgcatta
    13801 tatgagattt ataacacata taaaattttt tgtggaaaga tttttttcag atagtatgtt
    13861 aagcagtggt gatgatttat tatttaatca aatgaaaagg tcaaatccaa aagaaatgtt
    13921 aattgcatta aaagttagag attttttaga aaagaaatat ggaaaaaagt taacaaatga
    13981 ggaaatagct ttcttggttg tacatatcgc acgagttaag caatagagtg aattactaat
    14041 attatcttat acgtctacat ggtagaaaat tgaaagaagg taaagtatat aaagaaactg
    14101 agacatatta ttttgttgtt catta
//
LOCUS       DAGSHT010000080        14113 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGSHT010000080 DAGSHT010000000
VERSION     DAGSHT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.082"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     regulatory      complement(140..223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(456..1298)
                     /locus_tag="MJB72_002111"
     CDS             complement(456..1298)
                     /locus_tag="MJB72_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY8263218.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNTGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1550..3334)
                     /gene="pepF"
                     /locus_tag="MJB72_002112"
     CDS             complement(1550..3334)
                     /gene="pepF"
                     /locus_tag="MJB72_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBY8263219.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3400..4065)
                     /locus_tag="MJB72_002113"
     CDS             complement(3400..4065)
                     /locus_tag="MJB72_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBY8263220.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4216..4377)
                     /locus_tag="MJB72_002114"
     CDS             complement(4216..4377)
                     /locus_tag="MJB72_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBY8263221.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4455..4964)
                     /locus_tag="MJB72_002115"
     CDS             complement(4455..4964)
                     /locus_tag="MJB72_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBY8263222.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5170..5388
                     /locus_tag="MJB72_002116"
     CDS             5170..5388
                     /locus_tag="MJB72_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY8263223.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5406..7352
                     /gene="feoB"
                     /locus_tag="MJB72_002117"
     CDS             5406..7352
                     /gene="feoB"
                     /locus_tag="MJB72_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY8263224.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7531..8586)
                     /locus_tag="MJB72_002118"
     CDS             complement(7531..8586)
                     /locus_tag="MJB72_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY8263225.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8634..9434)
                     /locus_tag="MJB72_002119"
     CDS             complement(8634..9434)
                     /locus_tag="MJB72_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY8263226.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9427..10218)
                     /locus_tag="MJB72_002120"
     CDS             complement(9427..10218)
                     /locus_tag="MJB72_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8263227.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10258..10680)
                     /locus_tag="MJB72_002121"
     CDS             complement(10258..10680)
                     /locus_tag="MJB72_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBY8263228.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10696..11175)
                     /locus_tag="MJB72_002122"
     CDS             complement(10696..11175)
                     /locus_tag="MJB72_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8263229.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSNKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11307..14063)
                     /locus_tag="MJB72_002123"
     CDS             complement(11307..14063)
                     /locus_tag="MJB72_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8263230.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYENSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKREDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4571 a         2459 c         1603 g         5480 t
ORIGIN      
        1 cccttttcca acaaatggct actttaaaag ccaaaatttc acctatcaat aaaaatctta
       61 acgactaata ctcttatttt caaattttgc aacatttttg ttaattttaa aacaaaaaaa
      121 gactatgcaa tataaaaatt atattcatag ccggttgcac cactaactcc atatatctcc
      181 agatgcccaa taataagata tacttctcag tttctaaaat atttttatta aaaatctttt
      241 atatattttc ataaatttct tttttattct aacattgtaa tattattttg tcaatatttc
      301 catattctta gatataagat actaaaatac ctattttatc taaagatatt tatatttcta
      361 cattcaataa tagacaatac cctgcaagtt aaagcatcat ttttatgagc ttatctcaca
      421 gggtattata tgtttacttt atattataac tcttgttatt gattaacttt aataaatcct
      481 ttttctgaaa caagtgcttg tccttcatca cttaatatat aatctattaa agccttacct
      541 ttttctgaca aaccatcttc tttattaact aatataaatg gtcttgatat tggatattta
      601 ttagctatta catttgcatc agttgcttca acatcattta tttttaaact atttactttc
      661 tcatcaacat atccaaatga tatatatcct atagcatttt catttccttc aactgtagtt
      721 tttatatttc ctgaaccatc acttatttgt gcatctttag ttagttcttc tgaatcaaac
      781 cctacaattt cttggaatcc atctctagtt cctgaacctt cttctcttga tactattact
      841 attggtgcat catttcctcc tacttctttc cagttagtaa tcttccctgt atatatacct
      901 ttaacttggt ccattgttag attttttact gagtttgcag tattagttat caatgctata
      961 ccatctattg ctattttagt ttcctttaat ccagcacttt tttcttcatc ttttaaatct
     1021 cttgaactca ttcctatttc agatgttcct tctatagcat tttttatacc tccagaagaa
     1081 ccaatctgtt gtacttctac actaacacct tgattttttt cttgaaactt ttctccgata
     1141 gtttccataa gaggacctat tgaagttgaa cctgaaacag aaactttgct tgaatcacct
     1201 gaatcacctg aacctcctgt gttagagcca catcctactg ctaaagctcc aactaacatt
     1261 gtactagcta aaagaacgcc tattttcttt ttaaacattt tacttacccc cgcttatatt
     1321 ttaatttttt gttgtcattt ttctatattt taataataca gattctttgt taaatgaata
     1381 ttagagcagg gttaagtaaa tgtaaatatt gttaaataca tgtaaaaact taattttata
     1441 tgatttattc ttaattagat taacttttta aagttacttt aggcagatat aaacataata
     1501 caactcatat tatcagaagt cttcattgaa gctatcaacc tatcacatcc tataattctt
     1561 tttctagttt attaactaat tcagcaaata catttaaggc ttcctctata gattcttttt
     1621 tcgtcatatc tacaccagca gattttaatt gttctagtgg gtattctgac cctccacttt
     1681 ttaagaagtt tatatatcta tctactgcag tacttccttc tgaaagtatc tgttgactta
     1741 aagcacttgc agcagaaaac cctgtggcat acttatatac atagaagttt gaataaaaat
     1801 gaggtattct cgcccattct attcctattt gtttatctac tattgcagat tttccgtaat
     1861 acttttcatt taatttgtag tatatattag taaattcatc agcagtaagt ggttctccac
     1921 tctctaccct ttgatgtaca attttttcaa attcagcaaa catagtttgt ctatgaactg
     1981 tagttctaaa ttgttctaaa taataattta ataagtatat agtttcatcc ttagacttag
     2041 aattttctaa taaataattt ataagaagta attcatttaa tgtagatgca acttctgcaa
     2101 cgaatatctt atagtccgaa tataaaaatg gttgacttgt tcttgaataa tggctgtgaa
     2161 cagagtgccc taattcatga attaaagtga atagagaatt aaggtcattt ttatagctca
     2221 ttaatatata aggatgtgaa tcataacttc cccaagaata agctcctcct tttttacctt
     2281 cattatcata tacatcaatc cacccttcat caaaagcact ttttatgacc ttcaagtatt
     2341 cttctcctaa aggtttaagt gcctctaata tgatatcctg tgctttatca tatggtatat
     2401 ccatgtcaaa tttatctgtc aaaggaacat acaaatcata catgtgtatt tcattcaatc
     2461 ctaaaaattt ctttttaagc tcaacatatt tatttagatt aggaatactt ttatctatag
     2521 ctgatataag attattgtaa acatctaaac ttacatcatc tgaaaataaa gacgcttcta
     2581 ttgctgaatt atactttcta gtttttgcgt aaaatatctc tgattttatt ccaccataaa
     2641 gcgttgaggc aaatgtattt ttatatttct catatgttga aaattcacct tcaaaagcgt
     2701 cttttctaac tctactattc ttacttttta taaataaaga gtagtttgaa tgagaaagct
     2761 taaccttatt cccctcctca tcttctatct caggaaattc catatctgca tatgctaaca
     2821 tatcgtatac attttctgga attgtcgata attctgatgt agctgcaagt atttcttcct
     2881 ctcgttcact caaagtatgt ggtttatctc tcaatatatc ctttatatat tttgcataaa
     2941 aggatagctt ctcatctttt aagtattcac ttaacttatt ctcttctatc gccagtattt
     3001 ctggaactat ataagaagta gccacaccta aatcagtaga taacatttct gtcttagtgg
     3061 ctcttccttg attatcatta attctagtat cttcatgttg tttcatatga gtatatacat
     3121 ataaattttg aacaatccta gatgcatttt cagagatatt taatacttta tataagttct
     3181 ccttactctc agaaagcttc cctttatatt cttttacttc ttggataaga ttttttactt
     3241 tacttatatc tttttcaatt tcctctttac tggagtacat cttatctatc ttccatttgt
     3301 acttttcttc tattgtactt ctatcagttc ccatatcata cacctctatt ttaatttgtt
     3361 ttatatttta ttcactttac aaaatatatt gtatatattt tagtccaaat aatctacatt
     3421 tattactcct tctatcatat ttatttcttt cataacgtcg tgtaagtgta ttctcttagc
     3481 ataagataca aaaaaattaa ccttccagta ttcttcatta cgacattcta tttccatatt
     3541 tcttacgata agttgatttt tttcaaattt atcatataaa aaaggtatag aatccaaatt
     3601 gcttaatttt aatatcaata cattcttttc ttggctaaat aaaaacgtat tgattatcct
     3661 aagcacaaat aatgctatca ttacaaataa tccacctaga attgccaatt cataataacc
     3721 atatccaact gctataccaa tacatgcagt tgcccacatc ccagcagctg tagtaagccc
     3781 ttttactcca cctttatttt ttaaaattgc tcctgcacct aaaaaaccaa ttccagataa
     3841 tacttgagca ggcattcttg ctgggtcagc atttgtatac aaattataat caaagaataa
     3901 ttgaatcgaa gttattgaaa caatacaaga acctattgca attagtatat gtgtcctaaa
     3961 tcctgcaaac tgtcttattt tttctctctc taagcctatt attcctccta ttattagtgc
     4021 taatagtaac cttattgcta tctccttact tcctatatca atcataatat aaaaccctcc
     4081 tgacttaatt acaacttatg tcctacagat aatttacaca tcagaaatac tattttatat
     4141 cttaatatta ttattaatat atgtaaaata tagccatctg ttaatttttg atacaaaaaa
     4201 acccacaact gtgggctatt tgcttaggta catgtattta tttattaata tatctatttt
     4261 cttacttgat tctactaact cttcttgaaa tatttctgga tattctatgt atgtatttat
     4321 ctcttcccta actgcttcaa tctcattttt taacgcttct aattcattgt tcgccataat
     4381 aatcaccact attacaaatt tgtatatttt tatatacatt ttatcaaaca atattatatt
     4441 ttgtcaatat tatgtcaata aaacttacta taaaaatgct ttaatttagg tattgtatac
     4501 ctggatacta agcctacaaa cataccagtt actaatgatg ccattaacat aaatggtaag
     4561 taccctatag caagtatatt ttctatgatt aaacttgcaa ctattaattg acctatatta
     4621 tgccctattg cacctataat gcttatccct attagtgata aatcttttat tttactcgct
     4681 aaatacataa ataaaagact cagataagct cctcctatac taaatagtag atatgataaa
     4741 gggcctgcaa atattgatga caaaattacc ctgatagtaa caattataac agtatctttg
     4801 acacctaaca acattaatga aattaaagtt ataatattgc tcaaacccaa ttttgcccct
     4861 ggaaatataa ctataagagg atttggtata tatgtctcta atacatataa agctaaacta
     4921 taccctatca taagacctaa aaatgtcatt tttctagttt tcattaagtt aactcctcca
     4981 aattttatct atcatcttac attataatac aaaatatata aaaacaccta atttagacta
     5041 aaatattttt aatctattta gaataaatat tagttatttc aatgtgcaat tacctctata
     5101 aatcttgact ttttgtatca tacttgttac aattttaatg ataatgaatt tcattaggag
     5161 gtgttaataa tgactgttta cgatttaaaa ataggagaaa aaggatttat taatagtata
     5221 gatggagata ttaaattatc taaaagattg cttgcacttg gatgtataaa tagtactgaa
     5281 atagaagtta aaaaggttgc cccacttgga gacccaatta taattagatt tagaggcttt
     5341 gaccttgcga ttagaaaatc tgatgctaaa aatatttctt tacaataata gaggaggagc
     5401 tacccgtgat taatgtagca ttattaggaa acccaaatgt cggtaaaact actgttttta
     5461 atctacttac aggttcaaat caatatgtag gtaactggcc tggtgttact atagaaaaaa
     5521 aagaaggatt tcttggaaaa gagataaagg ttgtcgattt acctggtata tatgcaatgg
     5581 atactttctc aaatgaagaa aaggtttcta aatcatattt agaaaatgaa gatgttgatg
     5641 taatagtaaa tgtagttgat gcttctaatt tatcaagaaa cctttactta acaactcaac
     5701 ttatgcaatt taacaagcct atagttatat tgcttaatat gttagacatt gcagaatcta
     5761 aaggagttaa tatagatgcc aaaaaattaa gtgaagaact tggagtaatt gttgttccaa
     5821 ttatagcaaa aaagaaaaat ggtgttgata ggatagaaga agagattaag ggagctacgc
     5881 aaaaagcttt tatatatcag aaaaactttg gaagtgaaac agaaacatat aaaaaattag
     5941 aatccatttt atcaagatgt acaaaaacta catatacaca aaagaaatct atcagtgata
     6001 agatagacaa tatagtcttg aatcctatat tagcatatcc aatatttatt ggtgtattgt
     6061 tcctcttatt taaatttacc tttgactggg tcggaggacc actacaagag ggatttgcta
     6121 gtcttataga ggcttatatt tctgcacctg ttagtgatat gttatctaac tctagcccat
     6181 ggtttaaatc acttatagtt gatggaataa ttggaggtgt tggtggtact ttaccattct
     6241 tccctttaat atttacatta ttctttggta tatcgttgtt tgaagatagt ggatatatgt
     6301 caagaacagc atttttaatg gataaagtta tgagaaaggt tggtctatct ggtaaagctt
     6361 ttatacctat agttatgggt atgggatgtt cttctccagc tataatggca actagaacat
     6421 tggaaagcga aaaagataga agagttacag ctcttattgc accacttatg acttgtggag
     6481 caaagctacc tatatatgca ctatttgtag ctatattctt tccacataat gctgctttag
     6541 taactacatc actgtatttg gtgggtatag ttatggcaat tgtagttgca ttattcttaa
     6601 ataaaacagc ctttaaaaca gaggctgaac catttatatt agaattacca gaatatagaa
     6661 tccctactat agatgcactt attaaaaata cttggaataa atcaaaaggt ttcttgataa
     6721 gagttgttac tgtcatgttt gcaatgtcag tcgtaatttg gggactttct tcattcaatg
     6781 tttttggata tacagaagat ataaatgtaa gcttcttggc tactctaggt catattatat
     6841 ctcctatatt caaacctctt ggttttgatg attggagaac ttcagttgct attttaagtg
     6901 gtcttggagc aaaagaaatc gtagtaaact ctttaaatat actatatggt aatctaactg
     6961 ttgtcttacc aacaatattt aatggagtta ctgcatatac attcttgata tttactgctt
     7021 tatatactcc atgtatagct gcacttgcaa ctatgaaaaa agaatatggc aataaaatga
     7081 tgtttacatc atttgcatat caatttatac tagcatggat aatggctttt atagttaaaa
     7141 gtataggtgg agtactattt atgggtaatt caataataga agctttgata ggtggaataa
     7201 ttatagtcct tgcactcttt attctgttta ataaattcaa gtctacaaaa aacggaaatg
     7261 tctgtagtgg atgttctatt tgttctggat gtccaagtgc taattcttgc tcaagtaagg
     7321 aagacagcaa taaaaaaaat aatgcacact aatatataaa tattttagtt taaaataaga
     7381 taaaagtggg tgtctcaaaa ttaacttttt agttcatgag gcactctttt ttatagaaaa
     7441 tcaaatatat tttcatcatt tcaacagtaa aaaagagctt atctagtaaa aaagaggctt
     7501 atctctattt tgagacaacc tctttttttg ttacttattc agtttcttgc tacctaactt
     7561 catgtggaac ctagtatctt ttggaacact tgggtctaca cctcttaatg taggtatttc
     7621 cccacatatt agttgtatag gtactataaa attaaatgtt tgatagtaag gattatctgt
     7681 gatatcaatc tttaaatttt tatcattttc tgtaacatct ctacctatag caaatacatt
     7741 ttccgtccaa ccagaaagta catcaatcat cttagttact ctaggttctt ttcctgtatc
     7801 tagtataaat atagtagaat cactatttat agcattatat ataccatgta taaactcttc
     7861 aaactcatac ccagttacag gtattctcat tgtttccaat aattttaatg ctgcttctaa
     7921 tgtatctcca tatatatcag agtgacctat tattcttatt tctttagaat taactaactt
     7981 ttctttgttt ctttcaatcc attgtttaga gagcttatac actgcttcaa atctattaat
     8041 tgcgtctaat atcttattta tttcctcatt gtacttttca ctagatatta tacctttttc
     8101 tctagctatt tgaagaccta ataacattaa gtttaacttt gtacaataat atccttttgt
     8161 tttagctcca gatttttctt caccacaatt tactgttaat atataatcag aaatctcatc
     8221 tattaaagca ttcttacaac ctgccataga tgctatttta cagcccttat cttcagctaa
     8281 cttcatagcg ttgtatgtag aataactact cccaccttga gatacaccta ctactagggt
     8341 attttcatta tcaaacttga atgtatcttc agttatcata aatggataca ttttcacaac
     8401 atctatatct aataaatttt gtaaatatgg ttgcacttgc actcctgagt gatagctagt
     8461 acctgaacct gttattataa tcttttttag atttgttctc ttaacttcat taaataagct
     8521 atctgcatta gatataattt ctctcattct aactggtgtt tctaacatat aatcttgtat
     8581 tgtcatttta tacaccttcc ttatctatat attcaataaa ttataattaa tttctataac
     8641 aaacctatag catgtaaagc gattcctgtt actatagtgc ctatagttaa ttttgttaca
     8701 cttacctttc tctttaacat gtagtatatt acaaatgcaa aggttaaagg taacatatta
     8761 ggtattattt tatctagtat atcttgtaaa attacttctg caccatttaa gttgaatttc
     8821 agaggagtag ttatactcaa catagttgca accattcctc ctacaacgaa taaaccaaca
     8881 gttgtagcca tagacattat tttctccata agtccctctc tttgtattct ttcaagagaa
     8941 tttaccccta cttgataacc atacttaagt ccaaatattc taagggcaaa tgctggtata
     9001 ttgtataata gtaagaataa tagtggtcca aagatattac cttcttttgc aagtgatact
     9061 cctactcctg ctgctataat cctaaatgtt ccccagaata atgaatcacc aattcctgca
     9121 agaggtccca taagagctgt tttaacagca ctaattgaac tttcatcaaa ttctggatta
     9181 ttagcattct cttcttccat agctgctgtt atacctaata tagcaggaac tactactgtc
     9241 gttgtattaa aaatctccaa atgcctttct attgcttttg cttgattttc tttcttattg
     9301 tatagcttct ttattgcagg taacattgaa taacagtaac ctaagttttg cattctttcg
     9361 taattaaatg caccttgaag agcaaatgaa cgccaaaaca tacttcttaa ttcttttttt
     9421 gttaatttat tcaaagtcaa tctctccttc cgttaaagta ttgattgcta attgaggatt
     9481 ttgattattt tctttgttat tgatataaaa gtttagtaca aatgctacac atactccaaa
     9541 taaagctata gcagttatat ctaatccacc ataagatgct aacaaaaacc ctataaagaa
     9601 aaatacagac atcttttttg aaagtaacat atccataagc aatgcaaaac ctactgctgg
     9661 caacaatgtt cctgcaacac ccagtccgtc taatataact ttaggtattg catttattaa
     9721 tgctgtaact ttatcagcac caatcataat tgctaaaaag gttggaactg atgtacttaa
     9781 aaagaataat attactggca accacatcat tatggtaact ttcttaaaat cagcctgttt
     9841 agcatagaaa actgcctttt gagaaaagta cgaatttatt attctaacta atactccaag
     9901 agattgtgct aagactgcta ctggtactgc tactgctaaa gcaacttctg caccattacc
     9961 tgataatata gcaaaagctg tacctaatac acctcccgtt actacatctg gaggagtagc
    10021 tgcacctatt cctgcaattc ccatccaaat taattcaagc tgtgcaccaa tcataattcc
    10081 tgattttatg tctcctaaaa ctaatcctac taacatacct gtaactattg gcctatcaag
    10141 catagattga ccaaatatac gcccatctag tataccaact cctgctataa tagctaacat
    10201 agtagcttgt actaacatat tgaacctcca ttttaactta gtttaattta cattaaatta
    10261 cattacattt ttgattttgt tttgattttc taataactca tttacataaa caattccatt
    10321 ttttgcttcc tctaatcctg atttaactat taattttgta tcctcctcaa ctaataaaag
    10381 tatagataaa agcattggta aatttactcc tgtcaataat tcaaatttga acccttgtaa
    10441 aagtaagttt gtacatatgt ttgccacact tccaccaaaa acatctgtta agacaacaaa
    10501 ttcttcacta tctttattat ttgataatat acttctaatt tctttctcag catcatctgc
    10561 tgatttatcc gttgtcatac aaaaatactc aatattagtt aatgaaccaa ttattaattt
    10621 acttgtgtca acaagctctc tggacaattc tccatgtgtt gcaattagaa attttttcat
    10681 tctctttctc ctaaattatt ttattaaatc tgatagttgt ttctttttgt ctctaggtaa
    10741 cattctaaat tctatatcaa tccctaaatc acctatttgt ttaaagttat ttatatcatc
    10801 atcatcaaca gcaactaaat ctgtaagttt cttcttccct tcctcatatc ttaatcctcc
    10861 tacatttatt tcttttaata ctcctcctga atttttagct acaactaaag catctttggt
    10921 attttttact aacactaata cattgctttt atttgaagaa gcaaattttt ctactatttc
    10981 tccagattcc tctacccccc ttatatataa agttacattg ctaggtttcg ctaagttcag
    11041 tgacatagct ttcatttggt cattttttgc ttcatcattt gcaactagta ttacatttat
    11101 acctagtgat gttgcccatc catatgcaac ctgaccatgt atcaatctgt catcaattct
    11161 catcaaagat accattttcc ccaaatcctc catcatacat taaaattttt attgtttata
    11221 ctctcattta tctataaaag caaaaagtat gccaaatact ggcatacttt tttaaatttc
    11281 attcacttat tttgtaatct tgtattttac tgtggtagac catagataat attataaata
    11341 tatcctatct ctgaaattgg tatttgcaca ctataactct tttctagcac actaaatgca
    11401 ttttttatta gttttacttc actatgatgg tttttgatta acaaatccat atcagtatac
    11461 tcttcaattt cagtttgtct aactaatctt tcaatcatac aacttaagtg aacatataaa
    11521 gaaatctttt tatcatttga aagttttttc ttttgaatat tttctatagc attaattcct
    11581 tcttctattc tctctattat cttttttgaa tctaaaatag ttagactatc tattactcta
    11641 attaatgaaa aattctttac aatagaatca tttatgttct ttattttttc attttctact
    11701 actctgctaa atattctaaa aacatcatct tctctttttc ctgatattat atcctctaat
    11761 aatataaaat ctaccccatt cacatttgga ttggcagttc ctactatagc catcacatca
    11821 tacatttcaa aaactggttc tttatctccc atagaaacaa gactatcata atcataaggt
    11881 attattttga catcaactat tccaattaaa ctttcggaaa gtagatgttg gatttttata
    11941 gcagtaccta tccctgtaaa acaagatgat attatagcat attctttatt tttttgagga
    12001 taatacaatt gtatattagg tttagtttct tcatccataa tttctgcaat ctcttcaatg
    12061 tttttacctt gcattaataa atttcctact tctagtgcag aaatagtgga tatttggtct
    12121 ataaataaca ttgggctatt tatcttattc tttaaatctt catatataag attaagagag
    12181 cccatatcaa ataacacaat taaaccattc tttatcctat ctccctctag gtatctagac
    12241 atttgttttg atacatcagc aatagtagca tctataggca tatcaatagc tgtaaatata
    12301 tttttaccca acatccgatt acatacattc gccatactac tggcagtagc atatccatgt
    12361 gctataatag tcgcattaac ttgattaaat ttcattctta taattgttga tttaataaat
    12421 ataatcatta tgattgtatc tacattatta agatacatat ccaatctatt cgttattaat
    12481 gttgatattt tacttattat agagtattct ttgtaactct ctttttttaa atatattagt
    12541 aatttatttt ctaaagcttt atactcatca gacaattgat aagattcgtc cttcaaataa
    12601 agataagacg aaaatgccaa tacattattc ccatcatatt ttatattgtt attaacttct
    12661 aaatttctaa atatatcctg cattatcgat attataagct ccataagtac gttattattt
    12721 tcatcattat ttgaaaagac taaattgtcc attagtctaa tcattagatt agaactcttt
    12781 ttactaaatt tttcctttgt tatttcattt ttttctagtc tatcaaagta atctaaacat
    12841 tttatgaaac tttgatagat tatatttctt ttatttcttc catttgacat ttcaatacta
    12901 aattgtttag gagaaaagat aatgcttttg cttttaccac tttggaattg aaatatatct
    12961 acattttcta tataaagatt tctaggaagg tcttgaagag taacaaaaat atttccattt
    13021 cttctttgat tttttgcata agctcgtcca catgcatatt ttattacatt tttacattca
    13081 cctatattcc ccttgaattt tatctttaaa atcgcattta atgtaatttg agttatctct
    13141 ataggcaaac caagtacctt actttcttca taaataaaat gttctacaat ctttcttttt
    13201 tcaatagtcc ctctttcttc tatattggga acataaatat ttatgggtat tcttcttaaa
    13261 aaggtttgta aaaattcaga tagactttct gttgtagcaa atattaatct taccctagct
    13321 tttctaatta ctccattctc tccaatccta gaaaacgtac ctgtatccat aaatgtaaat
    13381 aacttttcct gaccttcact atttaatcta tgtacttcat ctaaaaataa aacaccacca
    13441 tctgattgtt ctattagacc tgctctagat tcatttgctc cagtatatgc tccttttaca
    13501 tgcccaaata atagactaga caataactcc aaattattat aatactgcgc acagtttaac
    13561 acattgtatg gagaattttt ttctaataca ttactgctta cactatattt gtatattatc
    13621 ttggctaaaa aagtttttcc tactccactt tcacctgtca gcattattgt aagtccgcca
    13681 tttggataaa gtacagcggt ttttatttgt tcgattactt cttttaagct attctcatat
    13741 cctatgatac tattaaacac agtctcttgc tttaataatt tacactcatt tttaagttca
    13801 tctaaatttt cataaatact tttttttgta ggtcctacta catcatttat tttattttta
    13861 ggtaaaaaaa taacaggtct actgtttatc tttataagat tatcagattt tactaaatca
    13921 tttaataatc tacttgattg agttctactt aaattaaact tttttactat atcttctgtt
    13981 attatgccac tttctttttt aagttcatca aagttgttaa ataatacaca ttcatttaca
    14041 aaacttaaaa tttcatccct catgttcaac ctccaataca aatatcaata atactataat
    14101 attagtatat cat
//
LOCUS       DAGSHT010000081        14027 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGSHT010000081 DAGSHT010000000
VERSION     DAGSHT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.083"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            515..3418
                     /locus_tag="MJB72_002124"
     CDS             515..3418
                     /locus_tag="MJB72_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8263231.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3451..3897
                     /locus_tag="MJB72_002125"
     CDS             3451..3897
                     /locus_tag="MJB72_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263232.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4020..4322
                     /locus_tag="MJB72_002126"
     CDS             4020..4322
                     /locus_tag="MJB72_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8263233.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4503..5909
                     /locus_tag="MJB72_002127"
     CDS             4503..5909
                     /locus_tag="MJB72_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY8263234.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6253..7311
                     /locus_tag="MJB72_002128"
     CDS             6253..7311
                     /locus_tag="MJB72_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY8263235.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7410..8096
                     /gene="araD"
                     /locus_tag="MJB72_002129"
     CDS             7410..8096
                     /gene="araD"
                     /locus_tag="MJB72_002129"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBY8263236.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEIRNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8114..8761
                     /locus_tag="MJB72_002130"
     CDS             8114..8761
                     /locus_tag="MJB72_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBY8263237.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8934..10301)
                     /locus_tag="MJB72_002131"
     CDS             complement(8934..10301)
                     /locus_tag="MJB72_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBY8263238.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10491..10579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11007..13127
                     /locus_tag="MJB72_002132"
     CDS             11007..13127
                     /locus_tag="MJB72_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBY8263239.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13231..>14027
                     /locus_tag="MJB72_002133"
     CDS             13231..>14027
                     /locus_tag="MJB72_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBY8263240.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYL"
BASE COUNT         5579 a         1593 c         2447 g         4408 t
ORIGIN      
        1 aatattttaa taaaacttca gttttaaatg tattcatatc gttttccctt ctgtatataa
       61 atttaatatt tataagttaa tttttaagtt aaaatagtat aatatgccat attaaatcat
      121 gttaaaacta tttatatagc taaaatctta cctatataag tatgtatact atcatataaa
      181 aataaaatct taaaatagaa agggttaaaa acacagttta ttataatttt tattgttgag
      241 tagatacgta ctattatata tgattgagca agtttttcaa tcataaattt ataaagtatt
      301 ttgcattaaa atttaaacaa agaattaaat tatttaagat tatgaataac taatacattt
      361 agtaaattac taaatataca aaatgtaaat acttatcaaa atgtataaat acttatgaca
      421 tctagttact tacagggttt aagtagaaaa aaatatcaaa gtaagtatgg catgatagtt
      481 gcttatatat ttatcaagaa aggagttatt aagcatggag ttgagagata aacttgtaaa
      541 catcataaat acagaaaata aaaaagatcc attaacagat gttcaaatag caaagttttt
      601 aagtacaact agagagaaca taactaattt gagaaaagaa cttaatataa gtaattcaag
      661 gcaaagaaga tatccttatt taaaatcagc aatttcggca atattacaaa agaataagaa
      721 tatttctata tcagaaatta caagggaatt gatgacagaa ggctttaata tatcgagaag
      781 agttgtggag gaacttttac ctaaagaaag tttagaaatg gatgtagtag aggaagatga
      841 agaagaatcg aatgacccat ttgaaactct gataggtaat aaagggagtc ttagaaatgc
      901 tgtagagcag gctaaatctg ctatattgta tccaccaaaa gggttaccaa cgttgattat
      961 tggggaaagt ggagttggta agagtttatt ttctagacat atgtatgagt ttgcaaagca
     1021 aaaaaagata gtaaaagaga gctccaattt tgttgtattt aactgtgctg attatagtga
     1081 caatccacaa ttattattat ctttgttatt tggatataaa aaaggtgcat ttacaggtgc
     1141 tgaatatgat acaccaggtc ttgtagaaga agctgatgaa ggagttttat ttttggatga
     1201 aatacataga ttgcctccaa aaggtcaaga aatactattt tctatcttag ataggggaaa
     1261 gtttaggaga ttaggtgaaa ctaatgctga aagaaaagta agtattatgt tgattggggc
     1321 aactactgaa aatgtagaga ctaatttatt attaacattc agaagaagga taccaatgct
     1381 aataactttg ccaccattac atgatagatt actaaaagag aaaatagacc ttatatacaa
     1441 tatctttcaa caagagtgta atagaataaa tgcaaaaata tttgtagata aaaatgtgat
     1501 tgaaatactt gcactaaaaa agtttagtgg aaatatagga caattacaaa atatgattca
     1561 ggttctttgt gcaagagctt ttatgaagtt tataaattcc aaagatgaag atagtgaatc
     1621 tatagtagta gtagatataa atgaagtttt aaagcttaaa gattcgttta aagatgctgc
     1681 ctttcaagag attgaatata cagagataag aaaatatttg aaaaatgcta tatttatacc
     1741 atttaattta gaagaatcat cagtaaaagg aagtctttta agtgatgaat atatattacc
     1801 agaggacata tacaaaacaa ttgaaaagaa atattatgac ctaaaatccc tagacattga
     1861 agatatagat atagagaata ttttatggac ttttatttta aatagatttg ctgacttaaa
     1921 gtttaatctt gatagtaatg aagaaatttt ttcaatgagt gatttaaata gttttgtaag
     1981 tgatagttta gttaaactgg taaaagaact tatgaatgat atagttgaaa aaaatatgaa
     2041 tttagaagta aataaaaata tgttcaaata tctagcaata cacttagaag aagctataaa
     2101 aagaattaag ctaaatcaaa agataataaa tgtaaattta gaaaaaataa agattgactt
     2161 ttcaaaagaa tatgaaatta gtaaaagttt tgcaataaaa attgaggaat caattggtat
     2221 taagatttca gatgatgaaa ttggatttat aacgctctac ataaagtctg ctctaaaaaa
     2281 tgaagtgaaa aaagataaag tagggttaat aataatttct catggacgta tagctacaga
     2341 gactgtaaat gtagttaaag aacttttagg tgtaaagttt cctgtggcta tagatatgcc
     2401 tttagatgaa aaaccaataa atatatacaa taaggcagta gaattgtcaa aaataataga
     2461 ccaagggaaa ggcatattgt ttttggtgga tattggttca cttacaaaca taggtcaaat
     2521 agtaaataag agaaccaata taagtactaa aactattgat agagtagacc tcctaatggc
     2581 actagaagca actagaaaag tttctatggg agaggaagaa cttgatgaaa tatttttttc
     2641 tttgctaaaa gataggatgg gatataacta tgaattagaa aaacacatag ataaatcaaa
     2701 tgcaattata acattatgtc ttactggaga aggaactgct aagtatataa gtaaaacgtt
     2761 ggaagaaaag tatgataaca ctaaatgtta tcaaatgagt gcattggatg aaagtttatt
     2821 ttgtaaaatt gaaaatctaa aagagacaaa caatatatta gctattgttg ggacaataaa
     2881 ccctaagata ccaggtataa attttatacc ctataataaa gaacttttca aaaatttgga
     2941 tatctatcta tcaaaatcaa aacaacaaga tgacagacca attaaatcgt atgaaagaat
     3001 gcttgatgag gatttagtga tttttgaacc agatatatat tttaaaaagg atttattaga
     3061 atatgtatgt tcaattctta taaataaaga atatgtagaa aaagattact tagattctgt
     3121 tttacataga gaagaaatgc ttccaacata ttctaaagga gctataggtg tgcctcatgg
     3181 agattcttct actgtaaata gtacaaggtt tgtttttgtt aaactaaaga atcctattga
     3241 ttggggtgta ggtaatgtaa atttcatatt tatgccagtt tttcaggcta atgataaaga
     3301 gattgttaaa aatgttcttg agatactaaa agatgcagaa tttatgaaca atgccaataa
     3361 gtgttttgat aaagatgctt ttagaaggat tatatttgat aaatttaaac atctgtagcc
     3421 atagcaaaaa aattgagagg agttagagta atgaatgtag atttaatatc attgggttta
     3481 gaatctaatg attatgaaag tgttattgaa gaattaggaa gtataatgtg taaaaaagaa
     3541 tatgtaaaag aaacatacat aaatgctgtc ttggaaagag aaaggacatt accaacaggt
     3601 ctagatattg gagacatgtg tgttgctatt ccacatacag attcaaagca tgtaaatgag
     3661 tctaatgtag ctgtagggat attgaaaaat cctgtaaagt ttaattcaat gatagaccca
     3721 aaagatagac ttgatgtaga acttgtattt ttattagcag taaagaaccc agactcacaa
     3781 gtaaaactgt taaaagattt aatgtcagtt tttcaaaata taaagttatt aaaaaatata
     3841 aaaaacgcat caacaaaaga agaagtagct aaattattgg attttataga aatttaatgt
     3901 aggagaattt tataaataaa aaattaaaat ttaaacaatc aaagtaagta ttattttttt
     3961 aataggttta atcttatata aaataaaaat ttagagtgaa aaattaggag gaaattatta
     4021 tgaaacaatt taatgtatta tcagtttgtg gttcagggac agttacatca tctatggtag
     4081 caggtaaatt aaaagaagta ctaggtaata aggggatttc tattactaca acagaagcta
     4141 gaccaacaga agcactaaat ttagcacaat caggaagatt tgactttata acatttacga
     4201 gtccacttca aacaggcgat tatggaatac caactataaa tgcatttgct tgcctaactg
     4261 gtatagggga agaggcattc tttgaagaag ttttgactgt tattgaaggg atagataagt
     4321 aagttttgga gacgtgaatt tatagtattt aaatcaatga tttagatact ataataagct
     4381 aatattaact aaatatttgt ggaaaaaaca ttttatatgt tgtcataaat taaaaaatta
     4441 taaaaatagt cacttaagta ttaaattaag cttataaata tttataagta ggagggaaaa
     4501 agatgttaga atttatgaaa tctataatcg atacatttgg ttctgcaatt atagttccaa
     4561 ttataatatt cataatcgca aagatattta aagtaactac taagaaatcg tttctttctg
     4621 ctgtgtatgc aggagttgct ttacaaggtt ttacactgat tttaaattca tttacaccag
     4681 taataacacc agttatcaat aggatggttg agagtacagg agtaaaccta ccagtttttg
     4741 atgttggttg gcaagcaaca tcattggttg cattttcaac aagtgcaggt atgatatatt
     4801 taggtcttgg tatattatta caaacaattt tatttttgat aaaatggaca gatgtgtttc
     4861 aaccatcaga tttatggaat aactattctt atatggtatg gggagcaatg gttataggag
     4921 taacaggaaa ttttccttta ggtatagcat gtatggtttt attaaaccta tatagtttat
     4981 taatatcaga gttagttgca aagagatggt ctagttacta tagatatcct aattgtacaa
     5041 taatagctat gcataatgtt gaagcttctg tgtttgcagt atttgcagac cctatataca
     5101 ataaattggg attaaataaa ataaaattaa atccaaaaga attagagaag aaattagggt
     5161 ttttaggtga accaattact ttaggtcttt ttttaggtat gtttataggt atattaggta
     5221 atatgacgag aataaatact atggaagctt ggggagaaat aatgaaagta ggtatatcta
     5281 caagtgcagt gatggcaatt ttccctaaag tagcaagtat gtttgctcaa gcatttgcac
     5341 ctataacaga agcagctaga aagattatgc aaaaagctgg aaaccgtgag tggtacatag
     5401 cagtaaatga tgctgttgga tatggtgaac ctgcaacttt aatatcaggt cttatattaa
     5461 ttccaataat gcttgtaata gctatggtac ttcctggtaa taaggtatta ccagttgtgg
     5521 atttattggc aattccatat atggttcaag gtttagttgc catacataat ggtaatatac
     5581 caaaagtatt agtttcaggt ataatttggt ttggattagg attatatgtt tgtacatcta
     5641 cagcaccatt atttacagat atggctacaa atataggtgt agctatacct gcaggagcaa
     5701 tgttaataac aagttttaat atactaggaa aacctttgat gggattagtt ttctttgcat
     5761 ttctaagtgc aaatccaatt tatataggtc tttcagttgt aatttacttt gttctatggg
     5821 cattgtttag aaagaacaaa acaagtattc ttgattattt agaaaaacaa gcacttaaaa
     5881 atgtagaaga agagcctgta gcagtttaag tttataaaaa tgaaataaaa ataattaaag
     5941 gagtttataa ttgattttat ataagtatca gataatacct gagaaatatt taaatgaaat
     6001 atagacttct ttttatttga taatatttgt aattataatg cattgtatct ttatagttga
     6061 gttttctgta taacctgcta tttaaaacat actcttttat atttatacag taatgtggtt
     6121 taattttata aaatgtaatc tctactggta aaagactttg tatataaggc atttgcctga
     6181 ataaaccatt tatcttttgt atggcataga tattgctata tagaagtata tagtatgaaa
     6241 ggagatattt atatgggaga taaaatgaga gcatcagtat tgtataatgt aggtgatgtc
     6301 agatatgaaa tggtagatat accagaaata acagatacgc aagtgcttgt aaatgtaaaa
     6361 tatgttggga tatgtggttc agatttacct aggtctatgg taagtggttt gagtggaaat
     6421 acaaaatatc cattgatttt agggcatgaa ttttctggtg aagttgttaa aattggagaa
     6481 aaagtaaagc atattaatgt aggagataga gtagctgttg caccattagt accttgtgga
     6541 aaatgtgatt attgtaatga aggtaatttt ggcttgtgtg atgattacaa tataattgga
     6601 acaagagtaa atggagcgtt tgcagaatat gtaagagttc cagaagaaca tattttaaaa
     6661 ctaccagata cattggatta tgaaactgct gccggaattg aaccagcaac tatagcttat
     6721 catgggattt caaagtctaa tattagagta ggagatagtg tagttgtgtt aggatgtggt
     6781 ccaataggtc agtttgttat acaatgggca aaggtgtttg gtgcatctaa gattatagca
     6841 gttgatattt ttgatgaaaa attagaactt tctaaacttc taggagcaaa ctatatactt
     6901 aactcaaaag aagtaaatgt aatcaaagaa ataaaaaaaa taacaaatgg tggtgctgat
     6961 gttgtaatag aaactgctgg tagccgattt acacaagaac aatctctatt tgtagcaaaa
     7021 aagagaggaa atatagtgtt tgtaggtata tcacacacag aattaccact tagtgcagac
     7081 gctactgagt gtatattacg tggagaactt accttgaaag gttcttggaa ttcatataca
     7141 agcccttatc ctggaagagc atggactgca acattggatt ttatgggaaa aggtgacatt
     7201 atttttaaac ccatgatttc acataaaata ggtcttaatg aagtgggtga ctttttaagt
     7261 aaaatgagca agagagaaat taatttcaat aaaatcctag tagaaatata aaaatttaat
     7321 aaaaaattct aaaagcaatt aaattttata actatgacat taaataaaat aaaaataatt
     7381 ttcttaaata aaatatagga ggaagaaaag tgttagaaaa tttaaaagaa gaagtattaa
     7441 aagcaaactt agaattacct aagaaaaagc taattactta tacttgggga aatgtaagtg
     7501 ggatagatag agaaaaagga ttagtggtga taaaaccaag tggtgtagag tatagtaata
     7561 tgactgtaga agatatggtt gtagttgatt tagagggaaa tatagttgat ggcaaattaa
     7621 gaccttcttc agatactccg actcatctag ttttatataa agagtttgaa gaattggggg
     7681 gaatagtaca tacacattca agttgggcaa caatttgggc tcaaatagga aaatctatac
     7741 catcttgtgg tactactcat gcagattatt tttatggaag tataccatgt accagaaaaa
     7801 tgactagaga agaaattcgc aatgaatacg aaaaggaaac tggaaatgta atcattgaag
     7861 aatttatggg aagaaatcca attcattgtc caggaataat tgtaaatgac cacggacctt
     7921 ttacatgggg aaaagatgca aatgaggctg tacacaatgc agtagtttta gaagaagtcg
     7981 caaagatggc ttattataca gagttaatga gtcctgataa tattatggat aaggttctta
     8041 tgaataagca tttttcaaga aaacatggaa aaaatgcata ctatggtcaa aaatgattat
     8101 ctggaggtga attatgctag actctttaaa aggtaaagtt gtggaaatag caattaaagc
     8161 agagcattat ggtttatgta aagaaaagac tggtaatttt agtattagag ataaggatac
     8221 tggatatata cttataaccc catcaggtgt aggaagagaa gaattaaaag ttgaacatat
     8281 ttgtgtattg gatttggatg gaaatgtaat agaagttgag aaaggtatta agccaagtag
     8341 tgaggtattg atgcatattg aaatatacaa aacaagagaa aatataaatg ccatatcaca
     8401 tactcattct ttatatgcaa cagcattttc agtagcaaag aaaccaatta taccaattgt
     8461 atatgaatca gttaattatg gcggttatgt agatattgct ccatacgaaa gaccaggaac
     8521 tttaaaatta gctaagagtg tttcaaaatt actgatagat acagatgcat gcttattaga
     8581 aagacatggg ttgacaactg taggtaatga tttagacgaa gcattattaa aatcaagata
     8641 tgtggaagag gtagcagaaa tatactatag gtctttagta ttaaatcaat ttaaagaact
     8701 tgaagtagta gatgttgatg agtttaaatc ttggaaatac ccagaagaaa taaagtttta
     8761 atctaaataa ttttcattga agtggttaat taaaatatat gtattattta tatcaattac
     8821 tatgttttat tatgttaatt accgtatttt gcaaatatat ttattacatt ttttcaaaaa
     8881 aatatagaat ccgaaagcca tataaaattg acttgggatt ctatactttt tatttatgca
     8941 tttttcttta tctgattttc tggagaattt atatgttcaa aatatttttt agtttcagat
     9001 actattatag gtgataatgc aagtaaagct ataaggttag gtattgccat aagcccattt
     9061 acaatatcag caattatcca aacaacttct aactttaaga aagcacctaa taacaccata
     9121 gccacaaata cagttctata cccattcatg ccttttacac caaacagaaa ttcaaaacat
     9181 ctttctccat aataactcca tcccaaaatt gtagtaaatg caaataatgt taaactaact
     9241 gtaagtaata ttggtccaaa tgtaggaatt gctccgttaa aagctgcttg tgtcataaca
     9301 gctccattta ggtctgaaca ccacactcct gaaataacaa gagagaatcc agtaagagta
     9361 cagattataa tagtatctat aaaagtacct gtcatagata taagaccttg ttccgctggc
     9421 cattcagttt tagcagcagc agcagctatt ggagcactac ctagaccaga ctcatttgaa
     9481 aatacaccac gagcgatacc atttctaatt gctagtgaca tagttgctcc taaaaaacca
     9541 ccagtagcag cagtaggtgt aaatgcactt ttaattatta acataaaagt ttcagggatt
     9601 ttattccaaa aagatattaa aatgattcca cagattacaa catatataac tgccataaaa
     9661 ggtactattt tagtagctac attagatata cttttaagtc ctccaaatac aactaaagct
     9721 acaaatactg ctaatacaac ccctgtaatt ctagggtcta tcccgaaact attatttatt
     9781 gcatctgtta ttgaatttac ttgagtaaaa gtaccgattc ctaaaagtgc aactagtata
     9841 ccacttatag cgaaaaatat tgcaagaggt ctccattttt ctcccatacc atttactatg
     9901 taatacattg gcccaccaga cacttgacca tttttatctt tagttctgta ttttattgca
     9961 agaacacctt ctgaatactt agtagccatt ccaaagaatg ctgctatcca catccaaaaa
    10021 agtgcaccag gtccaccagc tttgatagct gtagcaacac caacaatatt accagtacca
    10081 actgtagcag ctagagcagt acataaggct gcaaaacttg aaacatcacc actacctttg
    10141 ttttcagcag agaatattaa cttaagagct cttggcagtt tagttatttg aacaactcca
    10201 agcttaacag taagtagtat accagtacca actaatagta ccagtagggg tggtccccaa
    10261 ataaaagcat caactttgtt taaaatatca attagattca taaaaacact ccctcatttt
    10321 ttttgcttta cataatcaat agaattttta atgaagtaat tgacaggaaa taagtataat
    10381 ttattaataa tttctagtgt aataaaaaaa gctaaatcta aaaaagattc agctctggat
    10441 gtacactaat tagcaaccaa aataaaagta ttagtaaata ctgtttttgg tagataatcc
    10501 ctgtcctttt gcctgagaga ttgagcataa aagccttgcc ccttcggcgc ccataatggg
    10561 tctctccaga gttccgtaaa ttttacagtc ctacttttaa aagtacctga gagtgttact
    10621 ccttcggtgt gctatatagc aactcttcta taaaatctta accggatatt gatttgtcat
    10681 agcttattgt attaataata ttaatataag tttttatgtt ttgtcaacta aaaaattaaa
    10741 aaacaaagaa aaaatataaa aatgtaagcc tatttacgaa ttataaactt agaaagatga
    10801 gtaatatacg tgaaaatgaa ttttgtaata cacatacaaa tgaagtgtaa aataagtttt
    10861 aaataaaaaa ataataaaat cttaaataat taattagata tgattaaatt atggtaatat
    10921 tatagttacg atattaagaa aagttctatt tagttagatt ttaagcgtaa tatgagatta
    10981 tgtatagaga aaggatgtat ttttagatgg gaaaaatatt agtgctagca gaaaaacctt
    11041 ctgttggaag agatatagca aaagttttag ggtcaaaaaa tgaaaaaaat ggttatatag
    11101 aggggccaaa gtatgtgata acttgggcac taggtcattt ggttacactt gctgaccccg
    11161 aaagttatgg agaaagatat aaatcatgga gcttagaaga cctgccaata cttcccaagc
    11221 accttaagac tgttgtaata aaaaagagtg gaaagcagtt taatacagtt aaatcgcaaa
    11281 tgaatagaaa tgatatagat gaaatagtta tagcaacaga tgcaggtcgt gaaggagaac
    11341 ttgtagcaag gtggataata gaaaaatcac aggtaaaaaa acctataaaa cgtctttgga
    11401 tttcttcaag tacagacaaa gcaataaaag acggatttgc aaaattaaaa agtggaaaag
    11461 aatatgaaaa tttgtattac tcagcaatag cacgagcaga agctgattgg attattggaa
    11521 taaatgcaac cagagctttg acaacaaagt acaatgcaca gctttcttgt ggaagagtac
    11581 aaactcctac tcttgcaatg ttgcttaaaa gagaagaaga aataagaaat ttcaaaccta
    11641 aagagtatta tggattagaa ttaattgcta caaagggaaa ttcagatata aaatttattt
    11701 ggaatgataa aaacaataat tcttcaactt ttagtaaaga aaagatagaa tccacattaa
    11761 aaaaagtaaa aggtgtagac ataaaaatat ctgaagtaaa caagtcatat aagaaaaaat
    11821 attcacctgc actctatgat ttaacagaat tacaaagaga tgcaaataaa atgtttggat
    11881 tttcagcaaa agaaacttta tctataatgc aaaaactgta tgaacatcat aaagtactta
    11941 cttatcctag aactgattct agatatttaa catcagatat tgttggaaca ttaaaagata
    12001 gaataaaagc tgtaaatatt agtgattatt caaagatttg taatagatta ttaaaaagca
    12061 atattatagc taataaatcg tttgttgaca attcaaaggt aacagaccac catgcaataa
    12121 taccaacaga acagaaggta ttcatgagtg attttagtga taaagagcga aaaatatata
    12181 atcttgtagt aaaaagattt ttagcagtac tatctcctcc ttttgaatat gagcagacaa
    12241 ctataagtgc aactgtagaa ggagaaatat ttaaggctaa gggaaacaaa gtaataaatt
    12301 taggttggaa agaaagttat aaagatattg aagatgaaga tgagtatgaa agtataccaa
    12361 atgttttaga aaaagatgta cttaaggtca agggattaaa gataacttca ggaaaaacaa
    12421 atccaccacc atacttaaat gagggaacat tattgactgc tatggaaaag aatggtctag
    12481 ggacagttgc tacaagagca gatataatag aaaaattatt caattcattt ttaatggaaa
    12541 agaaaaataa agatatatat ataacatcaa aaggaagaca attattagat ttagttccag
    12601 tagacttaaa gacaccagaa cttacagctt catgggaaaa gaaattattg gatatttcaa
    12661 agggaaaact tagtaaaaat acattcataa atgacatgaa agattattca aaagatattg
    12721 tactagaagt aaaaaatagt gataataaat ttaaacatga taacttaact aaaaatagat
    12781 gtccagaatg tggaaaattt atgttagagg taaatggtaa aaaaggtaag atgttgattt
    12841 gtgaagatag agaatgtaat actagaaaaa ctatatcaca agtaaccaat tcaagatgtc
    12901 ctaactgtca taaaaaatta gaacttcgtg gagaaggtga ggggagaatc ttcacttgta
    12961 gttgcggata cagagaaaag ttgtcttctt tcaacaagag aaagcaagaa gaaaaacaga
    13021 aggcatcaaa aaaagatgtt aatcaatatt taaaaaatca aaataaaaat caagagacat
    13081 ttaataatcc atttgcagaa gcattagcta agctaaaaaa caaataattt taaagttatt
    13141 attatacaca attataactt taactgtata ttagttatat gtactgatat aatatagtgt
    13201 atagtttagg catgaaagta gggctgttaa atggatttaa gtggcatatt taaatactat
    13261 tgtaaagagt gtgaaaatac atggaataat tcgagtgttg aattatttga gaatatagaa
    13321 acgtatagta aagattcaca aaaaaagagg gaaaaagaat tagataaatt gctaaataca
    13381 atatcagttc atttagagag gtatccaagt gatgctgtat tgagaaaaat gtgggtaaaa
    13441 aagggcgagg ttttcttaca aaagacattg gaaaaagaaa atatttttaa gttagaaaaa
    13501 atggatgtag aggatagaaa aaaattttta gatataacaa aacagtttat tagagatgct
    13561 agaaaatttg atgatgattt acctataggt gatattatgc aagctatgag gaatgtatgg
    13621 atttcaaatg cattacaatt attatttggt aaagaagtat attattcaaa agctaacttt
    13681 gcatatagta tgttatatcc atatactgat aattatttag acaatacaaa tatagataaa
    13741 aatgataaga ttttatttaa taactggtta gaaaaaaggc tcctgggaga acacattaaa
    13801 tctaaggatt atcatgaaag taaagtatct caaatgatag attatattga aagtgtatac
    13861 cctagagaaa agtttacaga agtttatgaa tcgttattat taatatttaa aagtcaagta
    13921 aatagtttaa aacaacatgg taaggaaaat catttgtgta aagaagattt attatccatt
    13981 tctattgaaa aaggaggttc atccgtttta gtagatggat atttaat
//
LOCUS       DAGSHT010000082        13786 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGSHT010000082 DAGSHT010000000
VERSION     DAGSHT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.084"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..491
                     /locus_tag="MJB72_002134"
     CDS             <1..491
                     /locus_tag="MJB72_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY8263241.1"
                     /translation="ELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLIS
                     VIIGITNIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGD
                     SIGISAFWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSD
                     YM"
     gene            608..1390
                     /locus_tag="MJB72_002135"
     CDS             608..1390
                     /locus_tag="MJB72_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBY8263242.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(1509..2162)
                     /locus_tag="MJB72_002136"
     CDS             complement(1509..2162)
                     /locus_tag="MJB72_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263243.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(2186..3550)
                     /locus_tag="MJB72_002137"
     CDS             complement(2186..3550)
                     /locus_tag="MJB72_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBY8263244.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            4208..5638
                     /locus_tag="MJB72_002138"
     CDS             4208..5638
                     /locus_tag="MJB72_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8263245.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            5741..6844
                     /locus_tag="MJB72_002139"
     CDS             5741..6844
                     /locus_tag="MJB72_002139"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBY8263246.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            6859..7095
                     /locus_tag="MJB72_002140"
     CDS             6859..7095
                     /locus_tag="MJB72_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8263247.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            7112..8161
                     /gene="vorB"
                     /locus_tag="MJB72_002141"
     CDS             7112..8161
                     /gene="vorB"
                     /locus_tag="MJB72_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY8263248.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            8163..8903
                     /locus_tag="MJB72_002142"
     CDS             8163..8903
                     /locus_tag="MJB72_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY8263249.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            8906..9445
                     /locus_tag="MJB72_002143"
     CDS             8906..9445
                     /locus_tag="MJB72_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY8263250.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            9569..11668
                     /locus_tag="MJB72_002144"
     CDS             9569..11668
                     /locus_tag="MJB72_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBY8263251.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLYTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPLLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(11935..12450)
                     /locus_tag="MJB72_002145"
     CDS             complement(11935..12450)
                     /locus_tag="MJB72_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBY8263252.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(12542..13753)
                     /locus_tag="MJB72_002146"
     CDS             complement(12542..13753)
                     /locus_tag="MJB72_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBY8263253.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNISNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         5154 a         1750 c         2401 g         4481 t
ORIGIN      
        1 tagaattaac tcatagaagt aactatacat ttggaagatt tttaggtggc aaaatattag
       61 attcattcat tataggtatt ctaacatttg taatacttac attagttaaa atgccatata
      121 cattattaat atcagttata attggtatta caaatattat ccctttcttt ggacctttat
      181 ttggagctat accatccacg ctaatagtga tgtttgtatc tcctataaaa gctttctggt
      241 tattgttaat tatattaata atacaacaga tagatggaaa cattatagga cctaaaatac
      301 ttggagattc aattggtata tctgcatttt ggattttgtt ctccttattg gttgcaggaa
      361 aattattagg ttttattgga atggttatag gagttccaat gtttgcagta atttattcta
      421 taattaagga tattgttgaa agtaaattgg acaaaaaggg attaccgaca gatacttccg
      481 attatatgta gctaaaaaat attatgtata attaaaaata tataaattaa gcaatattta
      541 aagcactgag aaagttaaac tcttggtgct ttttagatat aaaattatta tagaagaggt
      601 gttttttgtg gcatttatag aatgtaaatt taaatctaaa tatactggag gagaaactga
      661 tataattgta attttgcctg tagcaaatgg aaaagaactt tttagtggta aagagatata
      721 tgattttgaa ggtaaattta aaacattata cttatttcat ggtttagaag atgaccaaag
      781 ctcatggatt agatatacta atgtagagca ttatgctaaa aataaaaata tagccctagt
      841 aataccaaga gtagaaacta gtttttatac cgatatggcg tatggacacc aatattttac
      901 ttttgtaagc gaggaattac ctaaatttgt tagagctata tttcctttat cagataaaag
      961 ggaagataac tttgtagctg gtatgtctat ggggggatat ggagctttaa aattagcgct
     1021 ttcaaaacca aaagagtttt cggcagttgc aagtttttct ggttcgcctg atatgattca
     1081 tgagttagaa aatcaacaaa tagaaaccaa tgcagacatt ctatttcacg attttggtag
     1141 accagaagac gctagaaata gtgagaatga tttactttac atacttaaga accttaaaga
     1201 aagaaatgaa gatattccta aaatttatca attttgtggt gatagagatt ttttatataa
     1261 aaataatcag atgtttaaaa aatatgcaga ttctctgggt gtagatgttg aatatgaaga
     1321 gtgtgtaggg gggcatgaat ggcgtctttg gaatttatgg ataaagaaat ttatagatat
     1381 aattggatag tacacttatg gtattaacag gtatactata aaatatcttt aaatatgata
     1441 aagactgaaa tcctaaaatc aaattatgat tttaaaattt cagtctctaa gaatttaata
     1501 aaattttatt aagttcttac acgagttatt attggagtaa aattgtcaac tatatctata
     1561 tagtatttac tcaattcctc taaaatctct tcgcataaat ccgtactatg accaagatta
     1621 ataagaaact gttttgtaaa atgctttatt acagcttgag aagatatatc gtacttgtgt
     1681 gaagtataaa ctctaattat ttcaggtttc atagcaagta taccaataca agaaagtatt
     1741 aattcatcat gttctataga ttctttatca ttaacatatc ttttcatgac catttcggtt
     1801 acacttcttt gatagcttaa aatagcttct tcataagcta tatccaaata aaaatttcta
     1861 tatgcttcgt tttttagaag gaaacaaagg tcagcattca tagcaagaaa gaacatcatc
     1921 atagtacctt cttcatggta gttatagatt aaagactgca tttcatttct tatatcttca
     1981 tatattatag ctcctatttc gcttttactt gagaagtaat agcttattag acctaaattt
     2041 atattagctc tttctgcaat ttgcctagtt gttgtcttat gataaccttg ctcaaaaaat
     2101 aattctctag cagcacttat aatactagct tttgtttctt tgcccttttc tgattttgac
     2161 atatttactg tgtatacctc tcttttcaat ctttttttga aaatttgcta actataaata
     2221 ttgtaataga accacctatt ataggaacta aaattctgtc aaatacagta agaataccaa
     2281 tcatatttaa taatgccaat ataacactta ctataacagc atagatagct cctttttttg
     2341 tagcaccttt ccagcaaaga ccaattacaa atggtgcaaa tattacacct gtacataatg
     2401 gagctggcca aaacaataat tctaataaac tatcaaattt aagtgctatg attgcaccta
     2461 ttatagctgt caaagctgat gcaatttgag aatactttag agtatttttt aaatcattaa
     2521 attctatatt aggatgtaat aatttgttat aaatatctct tgatataata gcactcattg
     2581 aggtcattaa tgaatcggca gtagacatta tggcagccaa tatagcagct aagaataata
     2641 cagatattaa aggtggcaaa gtagtcgata tcatttgagg taaaacctga acagatgata
     2701 tatttggaaa taaactacca ccaatagctc caattattcc tatagggaaa gcaagaaata
     2761 atagtataaa tcctgcaatt acactacttt taactgcatt tttagcatta tcagcacatt
     2821 gatatcttaa aaaactttca taagaaaata ttgagcctaa aactaatggt aaaactgtat
     2881 acataaaatc actaatcgat ttatttcctg gaaagaatgg ttcttttacc attgattgta
     2941 acataagtaa agggttgtcg ccaactaatt ttaagcttat ataagctact attggcaaaa
     3001 acactataca aatagcaagt tgaataaatg ctgatactgc aactccccac attcctgatg
     3061 tataggtata tataattata actataactc ctataattac agctatatca taaggaatac
     3121 caaatgcagt agatacagaa ccaaaagaaa ttaattgact acccataata gctagtatgg
     3181 atattggtgt tacaagactt tgataagctc ttaaaaatac actatgacca tatctagttt
     3241 caatataatc agcaggtgtt atatcacaag ataagcttct taattttcct gcgaaaaatg
     3301 catttataaa gcaagctaaa gctgcaccta tagtagaata cattccaacc caaagtcctc
     3361 tttcagctcc ccattgaact ccaccaacta agcaccctcc tccaaaagat gtagcagcaa
     3421 tggttgcagc tagtataggc aaaggcagat ttctacctgc gattaggaaa tcggatgctg
     3481 ttttaacttt ttttgaaaaa taaaaaccaa tccatatgta tattgcacac gtaacaatta
     3541 aaagtaacat agtcttttct cctttataaa atattttaat aaaatataca aaatatattt
     3601 aaaatatatt ttatataaaa gtagtacgat atttgtttta ttggttttag attaatatag
     3661 ctataataat ttatgatatt agatggaaat tacgtaaaaa tagaaaattg aaaattctga
     3721 aatttttgca tattgatatt tatctcatag attctttttt gtaattgtaa cacgctttta
     3781 tttttaaatc aatagtacat gtataaaaat aaaaggaatt taaataagaa aaagatattt
     3841 ttaatatctg gatgtagtat agaaatatct tgaataaatt attcttgaat aaaattataa
     3901 aaaattaaaa tcactagaaa tatgataaaa taatttaaaa tacgcatttc aatgacattt
     3961 caaaacaaga aaataataaa aaacaaacct aaattaagtc taaatagctt tttagttaat
     4021 tttagtagta aaaaataaaa cttaaaaagt gtttgacaat tatgaatttt gagaatataa
     4081 ttaaattaaa attatacaca tataaaaata tattcctgaa tcaagaatga gatatttaag
     4141 attcaaaata tatattatta tacgtgaata ataaaaagta agaaaatttt ataagggagg
     4201 ggtagatgtg agtagtcaag aaagcaaacc aaaaggaaat cttatatcaa attttagaga
     4261 tgatatgaga gaccctaaga aaagagcaat atacacatat tcaagttcta taaacttagc
     4321 gtacaatttt ataggttact ttgtatcaac tgttacaaat atttttttaa ctgatgtggc
     4381 aaaacttggt gtaattatgg caggtggtat gcaaattata caatctttca taaaaattgc
     4441 tgtagctcct ataacaggta cagtatttga caaacaacca ttcaaaaaag gaaagtatta
     4501 tccatggctt agatatgcac cagcagcgtt agcattaaca tatggaatat ttttcatagt
     4561 tccattaatg aaaataagtc cagcattact tttacctttg atgacagctt tattattaat
     4621 agcatcttgt gctcagcaag tagttttcac tataatttat gctatttacc ctgtagttgc
     4681 taagacacct agagaccgag ttataggctc aactactacg aatatattta aagaagttgg
     4741 taaattctta gtaggttttt catatccgtt attattagtt ttatttgcag atgtattagg
     4801 tggagaagga atgggttatt taggtaccta cttattcttt gcagcaggat gccttttagt
     4861 attttggttt tcttcaacag agataataaa atctggagca gaagaagaag ttcttcaaaa
     4921 agaagagaag ccaaagatta aagcaagtga aatggcaaaa gctttattca ctaatgtacc
     4981 acttatgatg gcattttgtt tagaatttct tatatgtata agatctatag gattaggacc
     5041 tttggctcca tattatttta aatatgtaat agaagatgaa cgtggattag cagtattttt
     5101 atcaatcatg cctttggtaa gtgtagcatt catgtttttc gcaccagtgt ttataaaaat
     5161 ctgtagagag caaaaactag cttcaataat ttctttttct atatgtgcac tatgccactt
     5221 attggttgca tggactcctt gggggaaaac tactttagga gtaactatgt tgctggcaat
     5281 tggtggaggt ttttcaaatg tagtatcaat aataaatctt aatttctttg ccggttcttg
     5341 tgattatggg cattggaaat caggaaaaga tttgcctggt ttaagtatgt cactatatcc
     5401 agttgcaata caagtaggag tattacttgc aactactata agaacagttt taatgaactc
     5461 aatgggatat caagctgata tggtagtcac agaagcagtt aaatcaggtt ttataaatat
     5521 gatttcttat tctatggcaa tacctttaat aatagctgtt gttatagcaa tactatatcc
     5581 agtaagtgac aagaaattaa accaaataag agaagagtta aatcaaagaa atgcttagtt
     5641 aaaagaaagt taagtagatg gttaatttaa gctattaaag taagtagacg caatagcgtt
     5701 tatataaatg aaaatacata tacagagggg gcttttaaaa atgatagaaa aattattgag
     5761 aaataccgta aaagaattac atcaatatgt tccaggtgaa ccaatagaaa aagttaagga
     5821 aaaatatggt gtaaaagaaa taataaaatt agcttcaaat gaaaatcctc taggaccatc
     5881 accaaaagca atagaagcta tgattgaaat gctaaaacaa ggtcaacttt atccagagcc
     5941 agaagctaac gaacttagaa gaaaattagc tgaaaagcta gatttaaaac cagaaaattt
     6001 tattgttgct aatggtgcgg ataacgttat cactctaata ggtgaggctt ttataaatag
     6061 aggagatgaa gttatatatt gtaacccaac atttccatca tatagaactg caactataaa
     6121 aaatgaaggg ataccagtag aagtaccttt aacagaagat tataagtatg atttacaagg
     6181 tatattagat aagattacag ataaaacaaa attaatatgt gtatgtaacc ctaataatcc
     6241 aacaggaaca attgtggatg ataaagagtt agaagaattt ttaaagaaag tccctgaaaa
     6301 tattataact atattggatg aagcatatat agaattttta acagttccta attatgtgga
     6361 tggacttaag tatgtaaggg aaaattataa cgttatagta acaagaactt tctctaaaat
     6421 atatggtctt gcagggctta gagtaggata tggtattgct aaagatgaga taataagaac
     6481 tttatttaca gtaaaagagc ctttctctgc aaatcgtgtt gctataagtg gagcaacagc
     6541 agcacttgat gatgaagagt ttataaaaga aacatatgaa ttaaatagag ttggaatggc
     6601 ttactttaaa gaagagttta caaaaatggg atttgatgtt gtagattcac aatcaaattt
     6661 cctttatgta gatatgaaaa cagatatacc taagttattt gaagacttga aaaaaagagg
     6721 gtttgtaata agaccaagtg ctacacatgc acgtgtaagt attggaacta tggaagaaaa
     6781 taggaaattt gtagcagttt taaaggaaat attaaatatt aaaggagagc caagtattgg
     6841 ataatagata gaaatattat gaaacggggt gtagatatga acttaaaaat aaacgcagag
     6901 agatgtaaaa gttgtgaata ctgcgttata tcttgtaaaa aaggagcatt aaaaatatca
     6961 agtaagataa ataaagaagg ttatgctcat gtagaagttg atgagagtaa atgcatactt
     7021 tgtggaattt gctatcaggt gtgtccagat aatgtttttg aaataattaa agaagaagca
     7081 tgtcaagagg cttagaagga gttgggataa tatgaaaaag atgtggaagg ggaaccacgc
     7141 cattgcagaa gcagccctaa gaggaggttg tgaattttat gcaggatatc caattacacc
     7201 acaaacagag gtaatggaat ttttatcaca tagaatgtct gaacttggaa gaacttttat
     7261 acagtccgaa aatgagatgg ctgctatata tatggtatat ggagcatatg caagtggaat
     7321 gaggtctatg acgagttctt caggaccagg aatatcttta aaacaagaag ggatttctta
     7381 cttatgtgca aatcattacc cttgtgtaat cgtaaatgta cagagatggg ggcctggtct
     7441 tggctcattg gactcagcac agacagacta tctaagagat actagagggg gaggaaatgg
     7501 agattatcat ttgatagttt atgcaccaaa cagtattcaa gagactgtag atttgatgta
     7561 taactcattt gatgtagcgg agaagtacag agtgcctgtc gaaattttga cagaagcagc
     7621 tttaggtcaa atgatggaac cagtagaatt tccagagttt aagaagagag aaggagattt
     7681 aggttggact tatgatggaa gtaatagaga ccatgcaaag gttgcagata accaaaagcc
     7741 aactttttgt atggagaaac gtatgagaat aactgaaaat gaacaacaat gggaagatta
     7801 tcaagtagaa gatgcagaat atgtgtttgt agcgtttggt ataccatcaa gaacaactat
     7861 gaatgctgtt agaagactta gagaacaagg tgaaaaagtt ggaataataa gaccaataac
     7921 agtatggcct ttcccatata aagcatttga aaaggtaagt aaagatgtaa aaggatttat
     7981 ctcagtagaa tcaacagaca caggtcaatt agtagaagat gttgctttag cctcaaagaa
     8041 agtatgtaaa gaaaatgtac ctgtgtatgg tttatttagt ggaaatcata taccaaaaac
     8101 attgcaagta atggacactt atagcaaaat aaaatctggc gaaatcaagg aggtgttcta
     8161 atatggctac agaaaaatta acacctagcg ttgtttcaat accaaacaag ttttgcccag
     8221 gttgtggtca tggaatagta aatcgtataa tagcagaggt aatagaagaa catggatatg
     8281 aaaaaaatca tgtattaaca ttaggagttg gttgtgtctg caatatgaat tttagctgga
     8341 atggtgataa gatgcaaact gcacatggta gagcttcatc tacagctata ggagttaaag
     8401 tagcacttcc agatacatta gtaatgactt accaaggtga tggagatgct tatgttatag
     8461 gtctttctga aactttaaat acagcatata gaaatcataa tgtgactgta tttgtaataa
     8521 acaataataa ttttgcaatg actggtggac aaatgtcatg gaccactatg ccgggtcaag
     8581 tcacaacaac aagtgtaaat ggaagagata taaagactac tggtagtcca ctgaaagttc
     8641 ctgagatggt ggcaaacttc tttgatgtag catatgtagc aagaggttca gttcatagcc
     8701 caaaggaaat aattaatcta aaaaaatata taaaaaatgc aatagaagct caattaaatg
     8761 gagagggata ttccttagtt gaaatattag caccttgccc aactaactgg ggtgtatcac
     8821 tagagaaatc tatcaaatgg atggaagaag aaatagtgcc atattatgct cttggcgaat
     8881 ttaagcaaag ggatggtgaa tagttatgga aaaagagcta gtatttgcag ggtttggtgg
     8941 tcaaggagtt ttaactcttg gtctgattgt agctgagtca gcacttgaat tggggaaaca
     9001 agtaacttgg atgcctgcat atggtccaac tatgagaggt ggaaaagcct atagtgtagt
     9061 aaagttttct gatgagagta taggtgggcc tgatatggaa gaaatagatg ttttagtagc
     9121 aatgaataaa ccatctttag attatataaa tttagttaaa gaaggtggaa cagtagttat
     9181 taatacatct gcaatagatg agaatgtaac attaagagaa gatataagtg tagttaaaat
     9241 aaattgtcag gaattagctc aaaaagtaaa caatccaaaa gcagcaaata tagtagtact
     9301 tggagcatta attgctaaaa ctgggttatt tgaaaaagaa ttagctttaa aaactatgtg
     9361 tgatttcttt gaggaaaaag gaaaaggaaa gtttaatgtt caaaatgaag cagcattcat
     9421 ggaaggctat aataacgcca tttgataaat tacccttatc ttaagtgatt tatctcaaga
     9481 tttaataatc tttagtgcca agagaaactt catcttgaga taaaattcac ttaaaatcac
     9541 aactataaac atataggagc gtgaatttat gagtttggaa aaacttatta gaccaaaaag
     9601 tattgctata gtaggagtga ctgataaact tggttttgga agaagtgcag cacttagtat
     9661 agtaaaaagt aaggaaacag atagagtata ttatgttaat cccaaaagag aagaattgtt
     9721 tgggagaaaa tgttataaga cgatacaaga agttccagaa gtagtagact gtgtagtagt
     9781 atgtacgcct agaaatgtag ttccatctgt attaaaagat tctggagagt taggaacaaa
     9841 ggcagcagtg gtatatgcta gtggatttgc agaggaaggt acagaggaag gtacagattt
     9901 agaaaatcaa cttatagaaa tatcaaatac atacgatatg aaaatattgg gtccaaactg
     9961 catggggttg ctaaattgta tagataaggt taatctatgg gcaggaggtt ctaaatggga
    10021 tttatatact aaaaaacctg gtataggaat tgtaggtcaa agtggattta taactgctga
    10081 aattgtttca agtgattatt ttaatatatc ttatggtttt tcaacaggaa atggaaatat
    10141 agttacacta gaagatgcag tagatttcct tgtagatgat gattatgcat cagtaatagc
    10201 aatttacctt gaaggtttaa aaaatcctca aaaatttata gatgctttaa aaagggcagc
    10261 acaaaaaaga aagccagtaa taatattgaa atcaggaagg tcagaaaaag gtgctatttc
    10321 tgctgcttct catacaggaa atttagctgg ttcgagtaaa gcttttgaaa gtatttttga
    10381 aaaatatgga gtaatttctg tggaaaattt agaacagttt atgtgtttag cacaagcttt
    10441 tagtgtttta gatggtaatt tacctgcaaa ttctaatttt gcagcaataa acttctcagg
    10501 aggagaaaat actatttgtg cagatttagc agaagaaaat ggtgtagaac ttgcagaaat
    10561 atcttctgaa actaaagaag aaatgaaaaa atatcttcca ggatttgcaa cacctaaaaa
    10621 tccactagat gctacaacag cattattcca tgaaaaggat atgatagtag gtttattaca
    10681 tacctttgaa aaggatgata gtgttggatt tactatggta ggtgcaaata taagagatga
    10741 agaaaatgaa atgcatgaaa ccctttgtaa agctgtaagt gaagctagag aacaaggatt
    10801 gaaaaaacct gtgtttgcag ttcctacact tgaaggaaat agatatttag attataggac
    10861 tagattagaa gataatcaag tgcctataat gtcatcagtt ggaactacat ttacttgttt
    10921 taataaaatg gctaagttta tagattatga ttattcaaaa agaactttag aatttaaagc
    10981 tgttaagaaa agagagtcaa ataatgtagt agcattatca gaattagata gtaagattga
    11041 gatgaaaaaa tatggagtac cagttccagg acaaggtaat gcaaaaagta ttgatgaatt
    11101 agatgaaatc ttaaaatata ttaagtatcc tgttgtttta aaaataaatt cttctgaaat
    11161 attgcacaaa tctgatgttg gaggagttaa aataggaatt aagaatagag atgaggcagt
    11221 agatgcatat aatgagattt taactaatgt aaaaaaagca aaaccagatg ctaatattga
    11281 tggtatatta gttcaagaaa tggtagaaag tggtatagaa attattattg gtataactaa
    11341 tgatgaccaa tttggaccac tgttattagt cggtttagga ggagtatttg tagaggtatt
    11401 taaagatact acattatatc cattacctat aaatcatgat gaagctatta tgatgcttaa
    11461 aaaactaaaa tcatttaagc ttttaaatgg ctatagagga agtgagcctt gcgatattga
    11521 tgctcttgca gatatgatgg tcaaattagg aaaatatgct tatgaaaata aggatgaagt
    11581 caaggaaata gacttaaatc cagtgtttgt atatcctaaa ggaaaaggcg tatgtgcagt
    11641 agatgcctta atagttaaat ataaatagtt tataagtttt ggtattcaac cataattaaa
    11701 gcaccgactg aaaatcacat tttgtaagtt agatagtgtg attttcagtc accccaaatt
    11761 taatttaaaa atattttaaa atgaaagtat agttttaagt ttcattgtga aatagcactg
    11821 tataaacaat aaaatactac gtataaaacg aataataaaa ggtgttttaa tgataagaat
    11881 caatcttgat tttcattaaa acaccttctt tattatatta aaaattttta aacgttatat
    11941 tgtcacaggt gggtttgttg gagctgcata tgtacgatta gcaaattctt ggtcaacaaa
    12001 gaatagacca ccctctccag caagtttaac acgttttata ttgtcttcac aatttgtctc
    12061 ttcttctgct tgttcagata taaaccaaag taagaattga gaagttctaa agtctgattc
    12121 agagtttgct acagctgcta agttgtttat taaagctgta accttttttt catgagctaa
    12181 cgtacgctca agaatatcca ttgcagaagt aaagtctgaa tctggcattg ggatagcatc
    12241 aagctgaacc tttcctccta cattatgtaa gtatttgtaa aaaaacatag cgtggtctgt
    12301 ctcttcttta tattgaacat aaaaccaatt agaaaatcct ttaagtcctt ctgcttcaag
    12361 gtaagaagac atagaaagat aaagatacgc agaatataat tcatggttaa tctgctcatt
    12421 aagtaatttt tccattttag ctgaaatcat ttttatacct ccaaatttta aatgctctta
    12481 taaaatataa taatattaaa tggaaaaata ttcaataaaa gtgtttaaat attttacata
    12541 tttaataaaa atttatacta ccattaaaaa aattataact tacaatggct atagagccaa
    12601 ttaatggtgc atttgctcca aaatttgaat gccttacact tattttttta tatttagaaa
    12661 aacttgctga agcagtcaat ttttttaata atgtattttc tataaatgta ccaggtatat
    12721 taaagtcata gcctattatt atagaactac aatcaatcaa tgtaagtgta ttaactaatg
    12781 catatgatac gtatgtacaa aattcatcaa gcaaaaacat ggcaagacta tctttattat
    12841 ttgcctcatc tattaacttg actaaagaaa aatctttaca gttaattaat ggggagttag
    12901 gataaatatg gctcaatgat tcaattcttt taattagatt actgacactt gtataataat
    12961 ctaaacatcc attgttacca caactgcaca atggtccact aaaattaatt gatgtatgtc
    13021 ctatttctcc actttgtcct aggttgccag tatgaagttt attttctaga actagaccag
    13081 ctccaatacc attcattatg tgtaggtata tataatttga tatattttta cctaaaccat
    13141 acattttttc tgctaatgca cccgaattag catcatttat caaaaatatt ggcaaatcag
    13201 acatttcttt tataaaatct actatattta ggtttgatat atttccaaaa tctgggggat
    13261 ttagtatggt accatttata tcatctactg gacctattga agaaatacca attccaatta
    13321 tatctctctt gttgattttc attattttag taaacattct ttttattata tcttttaaaa
    13381 catcatttga tagacattta gggtaagttt cagaaagttg ttttacaata ttacctttta
    13441 aatctgaaat aattacctta catatatttc ttcttattag gattccacaa atacaaggcg
    13501 atatattaga aatatctaat aaaatgggtt tacgacctga tgaatgtgca tttaaagttg
    13561 atgactctat ttccctaatc atattttcat taataagttc agtaactaga tttgataaag
    13621 tcattttagt aagtcctgta gacttagcta aatcaactct ggacataggt gtatttgtag
    13681 aaagtaattt caatattatt aacttatttt taatttttac atctttgatg ttagaacctt
    13741 gtttgttact catcataaat ctccttaaat aaagttaagt gtttta
//
LOCUS       DAGSHT010000083        13773 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGSHT010000083 DAGSHT010000000
VERSION     DAGSHT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.085"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            522..2252
                     /locus_tag="MJB72_002147"
     CDS             522..2252
                     /locus_tag="MJB72_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBY8263254.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            2362..2442
                     /locus_tag="MJB72_002148"
     tRNA            2362..2442
                     /locus_tag="MJB72_002148"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2396..2398,aa:Leu,seq:tag)
     gene            2777..3292
                     /locus_tag="MJB72_002149"
     CDS             2777..3292
                     /locus_tag="MJB72_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBY8263255.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      3784..3888
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4098..6572
                     /gene="gcvPA"
                     /locus_tag="MJB72_002150"
     CDS             4098..6572
                     /gene="gcvPA"
                     /locus_tag="MJB72_002150"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBY8263256.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            6572..8029
                     /gene="gcvPB"
                     /locus_tag="MJB72_002151"
     CDS             6572..8029
                     /gene="gcvPB"
                     /locus_tag="MJB72_002151"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBY8263257.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINVLKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            8452..10809
                     /locus_tag="MJB72_002152"
     CDS             8452..10809
                     /locus_tag="MJB72_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBY8263258.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDDNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(10895..11212)
                     /locus_tag="MJB72_002153"
     CDS             complement(10895..11212)
                     /locus_tag="MJB72_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBY8263259.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(11796..13067)
                     /locus_tag="MJB72_002154"
     CDS             complement(11796..13067)
                     /locus_tag="MJB72_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY8263260.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSETFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         5213 a         1569 c         2295 g         4696 t
ORIGIN      
        1 aaataagggt ttgttttttc attataaatt aataacatta aagtagttcc tccatagaat
       61 tgataaaatt tttattatga atacaattaa ttttataatt cgacaataat ataaaaactc
      121 ctttaaatta gaaattttaa aaactacctt gcaaagttta aataaataat atatactcaa
      181 aatatgttta ataaaagtga atagaagggt agaggcgcaa taatctatca gtactaataa
      241 ggagttaagc acagagattt attaggaaag gagatattgc cgaagtttta taatcgatgc
      301 tttaagatta tattgctggg ggtacataga aaatatgtac aactgtcaca ggtttatttg
      361 tggtgagcta tcatttaagg aattatatta tatttatttt tactattgtt gatatattgt
      421 aatgtttaag ccttgagtgt ttaccactca aggctttttt agttttattg taacataata
      481 ccctgtacag ttcacaaaat taaaaggaga gggagattta tatgaggaac aaaaaaatta
      541 atattatttt tctgacgaca atcatgttca taatgtcaac agtaatggtt tttgcagaag
      601 aagatataga cactattgca ctagccaatg cagaaaaatt tgggatttta actttaatac
      661 caccattagt tgctataata cttgcattta taactaaaaa cgtaataatt tctttactta
      721 taggtattct ttctggtagt tttataatta aagctagtgg tattaatgtt tttgcgacat
      781 ttatacaggc atttttagat ttagtagaca gagctttagt atctttagca gacccttgga
      841 atgcagggat aatactacag gttttggcaa ttggaggagt aataaatctt gttgctaaaa
      901 tgggaggagc taaagctata gctgaagcac ttgctaaaag agcaaagtca gcaaaaggga
      961 cacagcttat aacatggttt ttggggcttt tagtattctt tgatgattat gccaattcac
     1021 taatagtagg tcctatgatg agacctgttg cagataaaat gaaaatatcg agagaaaagc
     1081 tggcatttat aatagatgcc acagcagcac cagttgctgg tttagctata atatctacat
     1141 ggataggtct tgaggttggt ttaatacatg atgcttttga aagtataagt atagatgtag
     1201 atgcatttgg tatattttta aatacaatac catttagatt ttacaatata ttaatattag
     1261 ctttcattgt aatatcagca ttattattaa aagaatttgg tccaatgaga aaagctgaga
     1321 taaaatctag aagtagaaaa attagtatag accttgatga aggcgtagaa gagttggatg
     1381 atttagctcc aaaaaatgga gtgaaattga gtgtatggaa tgctataatc ccaataggaa
     1441 cattgataat agttgcatta gcatcatttt attatagtgg atatacatct ataatgggag
     1501 gagacgataa ggctctgata caactattta caaattctcc atattctttt gaagcaataa
     1561 aagaagcatt tagtgcatct gatgcatcta gagcattatt ccagtctgca cttgttgcaa
     1621 gtttagttgc tattataatg gctgtagtaa aaaagatttt tacaatatca gaagcaattg
     1681 atgtttggat agatggaatg aaaagtttag taatcactgg tgtaatatta atacttgcat
     1741 ggtcattaag ttcagtaata aaagagttag gtacagctaa atttttaata cacctattat
     1801 caggttcact accaccattc ttattaccaa gtttaatatt tggtttagga gcaataatat
     1861 cttttgctac tggaacagct tatggaacaa tgggtatact tatgccactt gcaattccat
     1921 tagcatactc attaaatcct gatatgtctt atgtaatagt aagtactagt gcagttttaa
     1981 caggtgcaat atttggagac cattgttctc caatttcaga tacaacaata ctttcttcaa
     2041 tgggagctgg ctgtaatcat atagaccatg ttaacactca aatgccatat gctatattta
     2101 cagcagtaat cactattgta tttgggtata taccagctgg acttggattg ccaatatata
     2161 tagtattacc agtggcaata gctgctatat ttgtaggtat tcaaataatt ggtaagaaag
     2221 ttgatgaagc ggaaatagaa cttgtagaat aaagatataa ataagtttca ccttttatta
     2281 tcactatttt gttagttttt agataatttt aaaaagttat tgaaaataat ggcgaaatat
     2341 agtaaaataa aagagtgata agcggatgtg gcggaattgg cagacgcact agacttagga
     2401 tctagcgcca ttggcgtggg ggttcgactc ccttcatccg cactttacag tacttaattg
     2461 tactgaaaaa aactggggtt gctggctttc ggagtgggct atcccgtttt tatttttttg
     2521 tatatataat agattggcac tttagtataa ggtgcttttt ttataggcaa aaacagtaca
     2581 tttatttctg ttttattatc attcatctat tagaatatac tttagttaag aatgtgttaa
     2641 taagcagtgt tcacattctt gataaaatgt ggaataatat gtatttttac taagacggaa
     2701 atgaaataat taaaccatta aataatatta taagatgatg aataagtaga taaatcaaat
     2761 tttgtaggag gatattatgg aaaaatatat atggttgttt ccattacttt ttatttttca
     2821 tgatatggaa gagataatag gttttggaat atggcttaaa aaaaataaat caatgcttga
     2881 taagaagtat ccatttatca gtaaagttta tgaaaattat agtactgaag gcatggcatt
     2941 tgctgtgttt gaagaattta ttttatgcat tattttctgt atacttacag ttataacaga
     3001 gaatcagtat gtatatctac tatggttagg tagttttata gcatatacct tacatttggt
     3061 aattcatata gggcagtcaa tcataataag aaaatatata ccttcactta ttaccagtat
     3121 catatgctta ccaataagca tttggtgtat aagtaagagt atttatatag ttgattgtga
     3181 aatgagtaca acaattctgt acagtataat tggcattata attgtagcat tgaatttaaa
     3241 gtttgcacag tcgctgattg gaaaatttac aaagtggatg tcaaacatat aaattttagt
     3301 ttattggaca gttcaaggaa tcagaaattg gaattttata gtattaatat tttatagtat
     3361 taatataatg tataaaattt tatagcaatg tatttcataa gtataaagtg actagaattt
     3421 taagaaatat acaaattcta taaaaactaa ataatttcaa gtatatttta aaaatcttat
     3481 ttttcacaag tttaaaattg taaacgattt catcaagtct atgaatatac ttttaacatg
     3541 ttatagtaaa tctcaatata aatttacatt tggttctttg tgtgttattt taataataaa
     3601 ttaagtgaaa atgttatttt acattaatat ataatatctt tgcataagag tgaataatat
     3661 aaaagagaaa taattgataa taaaaactta acaaaaaaat taatacataa aaaaaaataa
     3721 atataatgtg ttgacattta attgaaatat tcatacaata aaattgaaaa taaatatttc
     3781 caaatgagat tagcgagaga gtcctttata cgaaaaggcg ccgaagaaga aatttaagaa
     3841 atgccaattt ttagagaagc tttcaggcaa aaatatcgct aattgatggg actctggaga
     3901 gacttaagtt aagatactat aagtcaccga agaagcaaaa taatattaaa ttattaaatc
     3961 tttcaggtat acaaacaggg atatataggc taggattatt ttccatgctg atatatcttt
     4021 tttttatgaa aaagtacagg aaataatatc aaagttactt aaatacagta ggtctaattt
     4081 tctagggagg gttatatatg aatgaattga aaagagtatc tttgtacaat atacacaaag
     4141 agcttggagc aaaacttgtt gaatttgctg gttgggaaat gcctttagaa tatgagggta
     4201 taaataaaga gcatgagaag gtcagaaaaa gtgcaggtat atttgatgta tctcatatgg
     4261 gagaggtaca gataaaggga gcagagtcag aaaaatttat acaaaaccta gttacaaatg
     4321 atataagcac attaaagata aatgacatta tatatacacc tatgtgttat gaaaatggtg
     4381 gagtagttga tgatttatta atatataaat ttggtgaaga agattattta ttagtaataa
     4441 atgcaggtaa tatagataaa gatgtagcat ggataataaa acaaagtgaa ggatacaatg
     4501 tagatataaa aaatatatct agtgaagtga gtcaattagc aattcaagga cctaaagcag
     4561 aagaaattct tcaaaaaatc acagatatag acttaaactc aataaaattt tataagtcaa
     4621 ttccatcaac aaaagtatgt ggttgtcctt gtttagtttc aagaacagga tatactgggg
     4681 aagatgggtt tgagatatac tgtaaaaata aatatgtaga aataatttgg aatgaagttc
     4741 taaaggttgg aggagaagat atatgtccag ctggtctagg ttgtagggat actttgagat
     4801 ttgaagcagc tctacctcta tatggacatg aaataaatga acacatatct ccaattgaag
     4861 gtggtttatc tatctttgtg aaaacaaata aagaatcatt tattggcaaa tcaattttat
     4921 caaaagaaaa agaaagtgga gctaaaagaa agttagtagg atttgaaatg caaggtaaag
     4981 gaatgcctag aaatggatat gatataagga ttggtgataa aacagttgga tttgtaacaa
     5041 caggatgtgc atctccaacg acaggaaaaa ttttaggtat gggaattata gattctgaat
     5101 atgcaaaagt aggaaatgaa ataggtatag caatcagaaa aaaagttgtt cctgctgtga
     5161 tagttaagaa acctttttat aaaaaacaat ataaaaaaga taatattata ttaaacaaag
     5221 aaaataaatt ttcatatata cctgctacaa gtgaagataa atcaaaaatg ttaaaagtag
     5281 ttggattaaa ttcagttgat gaattatttt cagatatacc agaagaagta aaattaaaaa
     5341 gagatttaaa tttggaaatt ggaaaatctg aattagaggt aagcaaaata gtaaaaagat
     5401 tgtcagaaga aaatttaagt ttagaagact tgacttgttt tcttggtgct ggtgcatatg
     5461 accattatat accatcaatt ataaaacata taacttcaag atctgaattt tatacagcat
     5521 atactccata tcaagctgaa ataagtcagg gaactttaca agtagttttt gaatttcaat
     5581 ctatgattgc agaaattaca ggtatggaaa ttgcaaatgc atcaatgtat gatggagcaa
     5641 ctgctgcaat agaagcatgt ataatggcaa tgaatcagac tagaaaaagt aaaatagtag
     5701 tgtcaaaaac tattcatcct gaaactctgt cagtattaag aacttactta caatataaag
     5761 attgtgagat agtagaaatt gacttttgta atgaatatgg gactacagat atagaaaaat
     5821 taaaagcttc tgtagacaaa gatacagcat gtgttcttat acaaactcct aatttctttg
     5881 gaattattga agaaatggaa gaaatagaaa agataactca tgaaaataag gctatgttaa
     5941 ttatgagtgt agacccaata tcattaggag ttttaaaaac accaggtgag ataggtgcag
     6001 atattgttgt tggagaagct caatcacttg gaaaccctct taattttgga ggtccttatg
     6061 taggattttt agctagcaaa tctaaatata ctagaaaaat gccaggaaga atagttgggc
     6121 aaagtttaga tgtagaaggt aaaattgcat atgtattaac tttacaaact agagaacagc
     6181 atgtaagaag agaaaaagca acttctaata tatgttcaaa tcaagcttta aatgctcttg
     6241 tagcttcaat atatatggct actatgggta aagaaggatt taaagaggtt ggtatgcagt
     6301 ctatgaaaaa agcacattat acctacaata aacttgtaca aactggaaaa tataagccaa
     6361 tatttaaagg taagtttttc aaagaatttg ctgtacaagg aaatcttaat atagaaacta
     6421 taaatgataa actcttagaa gaaaatatat taggtggtta taatttagaa tacaattatc
     6481 cagaattaaa aaattcaact ttgctttgtg tgactgagaa aagaagcaaa gaggaaatag
     6541 ataagttagt tggaataatg gagggattat aatgaaagaa tacaatagtt tgttgataga
     6601 tatatctaaa aaaggtagga aagcatactc acttccaaag ttagatatag atgatataaa
     6661 aatagaagat atgattgacc cagatatggc aagacaaagt gagttgaatt tgccagaagt
     6721 tggtgaatta gaattagtta gacattatac attattatca aataaaaact ttggagttga
     6781 tacaggtttt tatcctttgg gttcttgtac tatgaaatat aatcctaaaa taaatgaaga
     6841 tatggcagca cttccaaact ttactggaat gcatccatac caatcttcgg atacagctca
     6901 aggttctctt tctttgatgt atgatttatc aagaagactt gcagaaatta caggtatgga
     6961 tgaagttaca ctacaacctt cagcaggttc tcatggtgag tttacagggc ttatgattat
     7021 aaaagcatat catgaaaata gaggtgacca taagagaacc aaagtgataa ttccagattc
     7081 tgcacatgga acaaatccag caagtgcagc tatggcaaac tttgatgtga ttcaaatagc
     7141 atctgataaa aatggagctg tagacataaa tgtattaaaa gaggttttga atgatgaagt
     7201 agcagctctt atgcttacta atccaagtac tcttggttta tttgaaaaaa atataaaaga
     7261 aatagctact cttgtacatg aagcaggagg acttttgtac tatgatggtg caaacatgaa
     7321 cgctattatg gggataacaa gacctggaga tatggggttt gatgtggttc atcttaatct
     7381 tcataagact ttttcaactc ctcatggagg tggaggtcca ggagctggtc ctgttggagt
     7441 taaaaaagaa ttagtacctt ttttaccaat accagtaatt gaaagaaaag aagataagta
     7501 tgtactaaat tatgatagag aaaaatctat tgggaaaatt aaaaatttct atggtaattt
     7561 tggagttctt gtaagagctt acacatatat actaactatg ggaagggatg gacttaaaga
     7621 ggctagtgaa atggcagttt taaatgctaa ttatataaag gaaagtataa aagatgatta
     7681 tattttgcca atagatacct tgtgtaagca tgagtttgta ttaggtggat tgccaagagg
     7741 agaggccaat ataaaaacta tcgatattgc aaaaagacta ctagattatg gataccatcc
     7801 tccaacaatg tactttcctc ttattataaa tgaggcactt atgatagaac ctacagaaag
     7861 tgagagtata gagacattag atagttttat agaagctatg aaaagtgttg ctaaagaggc
     7921 aaaggaagaa ccagaactat tgaaaactgc tccacataat actttggtca aaagagtaga
     7981 tgatgcaagg gcagtaaaga aacctatact aacatggtca caaagataaa tagttaaaaa
     8041 gtagagattt aaagtataaa gagaattaaa tttataagaa aaagctatct aacttaatct
     8101 agtttagata gcttttttgt atattagtat atatggtatt tcctatataa agttttaaat
     8161 tatattagtg cttatataga atattgatat gatgattatg tatagatatt aataagaatt
     8221 gttgaattta tcttgcctta actttattta ctcttttata ttaagaaaaa cttaactaaa
     8281 atatagcgaa attcataaaa atttaggaaa tcttaagaat tataatattt aaatctttag
     8341 atttatattt tattatataa actatagaat aataaaataa aaaaattgag aattttataa
     8401 tatatttgta gatttgagta taaaaaagtg ctaagttgaa aggagtaaaa tatggataag
     8461 gttaatttag aaaggtatag ttgtgatata aatgttggtt taaataaaaa tcaacttcaa
     8521 agtagaatag atgataattt aattaatgca tttgataatg gaaaaaccaa aacatataaa
     8581 caaattttta aagacaatat atttacactt tttaatctca taaatattgt tttagtctgt
     8641 ttattagttt cagttggttc ttttaaaaat accttattta tatttattgc tgtaattaat
     8701 actattatag gagtaataca agagataaga gctaaaagat tattggacaa tctatctgtt
     8761 atagtttcaa ataaggtatc tgtcataaga gaaaaagaga aaaaagagat tgatgtacaa
     8821 gaattagtat tagatgatat tatgatttta aaaacaggta atcaaatttg tgcagattca
     8881 aaagttttaa atggaaaatt agaggtaaat gagtccttgg ttacaggtga atctgatatt
     8941 attataaaaa ataaaggcga ttttttatat tctggaagtt ttgtagtttc tggagaagct
     9001 tttgtaagag ttgaaaatat aggaaaagat aactatgcaa ataagatagc taatgatgca
     9061 aagatttcta aaaaacataa ttctcaactt agaaattcat taaatgcaat attaaaaatt
     9121 gtaggtataa taataattcc tctaggaatt atattgtttg caaaacaaca ttttggaaat
     9181 ggagattcta tagctacatc tgtagtaggt acagtggcag ctgtaaatgg tatgatacca
     9241 gagggattaa cattacttac gagcattgct ctagctgtag gaactataaa acttgctagt
     9301 cataaaactt tagtgcaaga actgtactgt gtagagactc ttgctagagt tgatacacta
     9361 tgtcttgata agacaggtac aattacagaa ggtaaaatgc aagttgatga tatagtaatg
     9421 ttggaagaag tggatattaa tgaaataatg gggaatatat gtaattctct caaagatgat
     9481 aatgcaacac taaatgcaat aagagataag tataatgttt tagatacgta taaagctaag
     9541 aatttaatac cattttcttc agaaagaaaa tacagtgggg ttacctttga aaataaaggt
     9601 acatatttat taggtgcatt tgagtttata tttaaggaaa aaaataaaaa acttaggata
     9661 gaagttgaaa gatattctaa aaaaggaaac agagtaatag ttttagctca tagtgattct
     9721 tatatggaag atgataatat tcctgaagat ataaagccac tagcttttat attgatattg
     9781 gataaaatta gagatgaagc taaggaaact ttagaatttt tttacaatga aggtgtagat
     9841 ataaaaatta tatctggtga caatcctata acagtaagtg aggtagctag aaaagcaggt
     9901 cttaaaacag ctagtaattt tattgatgca acaacactta aaaatgatag tgatatatac
     9961 aaagcagttg ataaatacag tgtttttgga agggtaagcc cacaacagaa aaaacaaatg
    10021 attttagcat taaaaaaaca aaatcataca gtagctatga ctggagatgg tgtaaatgat
    10081 gtgctagcac ttaaagaagc ggattgtagc attgccatgg catctggaag tgatgttact
    10141 aagaatgtgt ctaatttagt cttattagat tcaaactttg catcaatgcc aaaggttgtt
    10201 atggagggaa gaaaagtaat taataacatt caaagagcat cttctttgtt tttagtaaag
    10261 acaatttttt cattcttctt gtcgttatta acattattct tcttcagtag acagtatcca
    10321 tttgttccta tacaattatc actcattggg gctgtaacag tgggtatacc atcattcttc
    10381 ttagctcttg aagcaaacaa aagtagaatt tcaggaaatt ttttactcaa tatattaaaa
    10441 aatgctttac caggagcact atgtgtgaca gtaaatgtaa ttattgttta caatatagtt
    10501 gaatatttgg gttatagttc aagtgtattt tccacaatgt gtgctatatt aactggtgta
    10561 tgtggtcttt taatattaag acaacaatgt ttgccattta ataaagaaag attattttta
    10621 atagtaagta tgactattgc atttgttatt gggattctat ttttaggtgg attattctca
    10681 tttgtagcat tgagtagaga attaattctc ataactatgg cattagcaat attaatgcca
    10741 attatgataa aagttatgtt atttgtctta gatgagatat tagagacaat tgttccagat
    10801 ttaaattaat tttaaaatga tataaataag agcagtttta gttgaactgc tctttttaga
    10861 tgtgtttaat tttatgtaag aatatgtatt tttattaatc agctatatat gagcaacagt
    10921 agtcaacaac ttcattaacc tttaagtcaa aacttgaatc actaggaaca ttaaattttt
    10981 gtccttcttt gtaagttaca aactcattgc ttccaggcag ttttacatcc atacttccag
    11041 caagcatctc cattatttct tcttctctag tagaaaaagt gtattctcct ggcaacatta
    11101 atcctaaagt ttttctttct ccattaggca gtattattac tctgctactt accttaccat
    11161 caaaataaac gtttgccttc tttactgcag taacattctt aaattcactc attagataaa
    11221 acctccaaaa ttatttgtac atatatatta tataaaaata atgtaaaaaa tgctatatat
    11281 ttacaaaaaa atttagtatt tattaaaaaa attaataaga ataaaaaaga gatggaattg
    11341 cttaaattga taaaaaatat aaaataaatt gcaaagtaag caagaaaaga ataagttgaa
    11401 gagttatata ttataaatat taaaaatttt agtattaata aaaaagatat tacattaata
    11461 tgaaaatata ggagattaga agtgtttgat aattacagta catgttagat atatcattgt
    11521 aaattaacat agctttgaat ataataatgt tcaaaaggca atatttttga ggtctgaatc
    11581 caataataat atagtcttat gattgtagat atcaaaaaat ataagttact aagaatacgt
    11641 tttgtagatt tatatatgat taataactaa agctataaat aaacaaaata aaaagagtat
    11701 gtttcaacct atagctttta gatttaaaat atactctttt attaattata gttgtataaa
    11761 ttaaccataa tatataatta aaatttttat tataattata tattaggttc ttcaattagt
    11821 tttccacata catatctatg aatgatattt ctatcatcac caacatagat aaatctttca
    11881 agtctttcaa gtatagaata attgtcatga tttaaatttg aatcatcaat aatcaatgca
    11941 tcaaaatcat aaccttcttc aaagctacca acttttccaa agaaacttcc accacttttg
    12001 gttgcaatat aaaaagtctc agataaagat aagaaattat acttcttacc agagttaacc
    12061 caatataatt tagataattg tattgcagat accattgctt taaatattga caatgtatgt
    12121 ccaccactta tatctgatcc aagtgctaat ctaatattat tatcgattaa ttttctgata
    12181 ggcatagcac cactaccaac attaaagtta gaagctggac agtgaactgc cataacatta
    12241 ttttctctca ttaaatctat ttcatcctga gaacaatgaa cacaatgagc cattaaagta
    12301 ggagtttgac caaataaatt atatctatta tatgcatctc catagaattt agattcaggt
    12361 tctagacttc taacccattc aatctcatca taattttcag ataagtgtga ttgaatagga
    12421 atattatatt ttaaacataa atctcctaaa ccttttaaaa gttcacttgt acagcttggt
    12481 ataaatctag gtgttattat tggttttaca atagaatctg tatcactata cttatttata
    12541 atttcttctg tatcagtaat agaatcctga gtgttttcta ataaagtatc agggcaattc
    12601 atattcatat ttactttacc aacgtatgct ccaagaccaa cttctttgaa tatatccata
    12661 agtttcatag aggcttcttt atgtactgtt gcaaacactg ctattcttgt agttccagat
    12721 ttccaaagtt cttttacaaa ttttttatac atttttgttg catattctat atctttataa
    12781 tttgattctt ctggaaatgt ataagtttca agccatggca aaagctcttt atccatagcc
    12841 attccaagat ttttaaattg agaggcatgg gcatggaggt cattcatacc aggtattata
    12901 agcttatcag tgtaatctac tacttttagg tttttgtatt ccttaggtat ctctttaaat
    12961 attcccttta ctttaccttt ttccagtata atataactat tttcaaatac tgtgaaagtt
    13021 tctgaagttt ttgtaaaaat tatatttcct tttaatactt ttaacatata ggtctcctta
    13081 gttttaaatt tgttttaata tatgtaggta aaattttgtt ttacaaatca aaattttacc
    13141 acatgtatta tatcaaatct aaactataaa tactattaga taatagcaaa tattagtgaa
    13201 atacagttgt ttactattat tatatataac tgacatttaa gtgacattta agaaaaatat
    13261 aatgcctact tacataaaat ggaatgttat ttaaagagaa ctttgattat attttcagaa
    13321 gcttttttat ccatatcgtt taaaatatga gaatatctat ttatagttat ttttatatta
    13381 gtatgtccta atctttcaga gatgattttt atattagttc tagctagaag aagaactatt
    13441 agaatagata atatagtaag tatttacaaa tatgtaggtg ttcttaaatt gataaattat
    13501 tccattttaa ttttatagtt tgaattttat gatataataa aaatatataa attttgcagt
    13561 gagcgatatt tttgataaag tagggtttaa cagttgcaat gtaagggatt gagggtgtat
    13621 gataaatgtt atcaattgca ctactcatgg ttcactgcaa atttgagaga gttgtatgtg
    13681 tgtaagtact gaaaatactt agtttatttt ggggttttat attaactaag tggtatgtaa
    13741 atagaattta gtactttcta aaaatggatt gcc
//
LOCUS       DAGSHT010000084        13772 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGSHT010000084 DAGSHT010000000
VERSION     DAGSHT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.086"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(240..1034)
                     /locus_tag="MJB72_002155"
     CDS             complement(240..1034)
                     /locus_tag="MJB72_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8263261.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            complement(1568..2422)
                     /locus_tag="MJB72_002156"
     CDS             complement(1568..2422)
                     /locus_tag="MJB72_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBY8263262.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            complement(2454..2966)
                     /locus_tag="MJB72_002157"
     CDS             complement(2454..2966)
                     /locus_tag="MJB72_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBY8263263.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            complement(2959..3183)
                     /locus_tag="MJB72_002158"
     CDS             complement(2959..3183)
                     /locus_tag="MJB72_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263264.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            complement(3243..4196)
                     /locus_tag="MJB72_002159"
     CDS             complement(3243..4196)
                     /locus_tag="MJB72_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY8263265.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            complement(4224..5612)
                     /locus_tag="MJB72_002160"
     CDS             complement(4224..5612)
                     /locus_tag="MJB72_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263266.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            5886..6767
                     /locus_tag="MJB72_002161"
     CDS             5886..6767
                     /locus_tag="MJB72_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8263267.1"
                     /translation="MNTKQLEYFISVAEKLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(6955..7947)
                     /locus_tag="MJB72_002162"
     CDS             complement(6955..7947)
                     /locus_tag="MJB72_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBY8263268.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKALSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(7952..8692)
                     /locus_tag="MJB72_002163"
     CDS             complement(7952..8692)
                     /locus_tag="MJB72_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBY8263269.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            complement(8757..9866)
                     /locus_tag="MJB72_002164"
     CDS             complement(8757..9866)
                     /locus_tag="MJB72_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBY8263270.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDVVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            complement(9884..11017)
                     /locus_tag="MJB72_002165"
     CDS             complement(9884..11017)
                     /locus_tag="MJB72_002165"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBY8263271.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            complement(11019..12113)
                     /locus_tag="MJB72_002166"
     CDS             complement(11019..12113)
                     /locus_tag="MJB72_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBY8263272.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(12250..13551)
                     /locus_tag="MJB72_002167"
     CDS             complement(12250..13551)
                     /locus_tag="MJB72_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8263273.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
BASE COUNT         4333 a         2625 c         1527 g         5287 t
ORIGIN      
        1 acgcaatgtc agatatcact gttggaagtt ttaacattat agtcactact accaataaaa
       61 aaagtggtaa aaaaattata tttgttagaa taataaaact ggctataggc ctttttaatg
      121 atttctctgt tctcataagt aatcctcatt tcaaattaat atttaattgc tatataagtt
      181 ataactgctt atatcatgag tttaaaccta tgagtaagtc ccaggtcaat gcttttatat
      241 tattttatag gtgcataaat ctctaaaaat atacgctcaa tcccttcttc ataagtagta
      301 agcaatatat taacatagac tattccaagt aattcacatt cattttctat tgctatttct
      361 cttaatctgt cttccacttt attatcaata ttttctcctc cagttgacca tctgccatct
      421 tcaataatag tataaataca attgggatga gatagaactt caccttctat gattttgttg
      481 tagtcatcta cttcccttac aattataaat ttttcttcta ttataccttc ttcattaaaa
      541 tttattactc ttcttaaatc agttaatgta actgcttttt ttaagttgtt tgtattctct
      601 tttagggctt catattcctc atagttagta acatgtttta ttttaccttt tacttccaaa
      661 ggcttcattt cttttattgt gtattttcca aaatatgttt ttattttgtc acaatcttct
      721 ttgactaact gaaccttttt taagagaagc tttttatatt ctatctcttt tagtatgatt
      781 tgttccttct cttctagtaa gagttctaaa ttgtttatac tcttactttt tcttatttct
      841 tgaatctttt taatttctat atcaatttct ctataaaagt ttattgttgc aatatcatag
      901 atatcaacct gattatatat tctatatcca ttttcatcat ttttcttagg atttactaat
      961 cctttgtcct cataaaattt tagtgtatct ctagatattc ctaaaaattt agatacttgt
     1021 ccaattgtat acattatatt ctcctattat tagataaatt ctttatgctc attaaacgta
     1081 tttctattaa caattacaaa atcacctaaa ctttgaagta tcaaagtcag catagtattt
     1141 acaacctttg gaattactct ttttatttca taatccaagt gtaaacttgt taatatgata
     1201 cgttataaag actatattgt ctacatattt ctaacaatga ttctttcact ttaagatgtt
     1261 cttaaacata tttaactttt atttttaaaa atttcctata cataaaaata ttcctttcat
     1321 gataacagtt ttattatttc aactatctgc taccataaaa ggaatatctt aatctttaaa
     1381 cacattctaa atgctctttt aacaaatatt atcaaattat tctaattcta ttgtttcata
     1441 tttattagtc ttagttttat ataaatttaa atataaaact atatattcac ctacttttaa
     1501 ggatacaata agtcttcttc taccagtaag agtaaatgcc tcctctactt atcacaaact
     1561 ataattttta tctcattggt atatatccac gatactgtga gtatgcatcc atagttgcta
     1621 tatcacctaa gaatactgat gcgatattga attgtggttt taaatatggg aactcctctt
     1681 cttgtgccca ttctggtttt ttaataaacg actttgtctc ttcaaccaca gctttatacc
     1741 attcttcttc cccttcaaac catagttctg atacacggtc aaaagcagtt gctccataat
     1801 taatcattat tttactactg acaaaacgat taacatagca acagtttgta aagtatggca
     1861 ctacctcatc atagaaccat ttttctcctt cttctttaga gataccatct ggatacttaa
     1921 tcataaattg ccaacgatag tttggtccat cttgtacagt acgtcctttt cctctaaagt
     1981 cttcttccca gtttattgga acaaaggcaa atataaatgg tggacatcca ttatctccac
     2041 ctactgcacg tcctgcatct ccactttcta cattttcatg aaccctttta ttttctacat
     2101 ctggtataca cccttgccaa cgtagaacat ccataggcat atattctgtc ataattctat
     2161 ttgccatctc aggcatatgt tcatctacaa gccaataatg ttctgttagt tgcatcttac
     2221 gtgcaccaaa acgctcacct tcatttggtg taggatatgc ttgataaaag gcatatttag
     2281 tacaatatgg tccaaaagtg ctaatactat ctggaatatg gtgacgatat aaccagtcca
     2341 ttagcttagg tctatattct tccttggcaa gtcctacata tattaaactt ctcattattt
     2401 taaattcaaa acccatagcc attctcctat aatcaaagta tattcacatg ctttcaattt
     2461 ccctttttcc tatctttact caaaatatat attgtaaaca aatctacacc tgcacccacg
     2521 taccatactc ctactgccat agctacagca atttgaatac aaatatcttg taaattaata
     2581 atattttcac ctgtcataac agttagacct atagcccaac cacaaagcca tgatggtagg
     2641 aaaattattt tttcaaataa ggatgcaatt acaactgcaa aaaaacctaa tatgctaagt
     2701 gttaaaaata attctaggtt ttgattgaaa ttcatatatc ccataattaa aatggctaac
     2761 actgcccata aatctccaca ccaaaaacca aacattattt ttaatccatc tttttgttta
     2821 ttaccattag ttacatataa tcctgcacag attagtgcta cagctccagt ttttattcct
     2881 atataaggtg ctaatactgc ccataaagga ggtagtaaag caatccccat agacagtgtt
     2941 aatatttctt ttttctttct atccatttcc tatattgtaa ttagtagctt ccctagcttg
     3001 ccttatctcc aatagcttaa ttgctatttg atttacaatc atagatactg caaaataact
     3061 taaatatagt ggtataaata taacaataaa tgctggagtt aatctaacct tgtaatatgt
     3121 aagtaccaaa aaaataactg ctaaaataac ttcaatttgt ttaaaacttg gcttttcatt
     3181 catacaataa gcctcctata acatctaaca tcccttaaaa aagagtctta gtattttaat
     3241 tattatccta atgaaactcc agcttgcttt tctttagtta atggctctat aaaattataa
     3301 gatgtttttc tttctttaaa ttcttgtgtt ttacgaactt ctgcataatg accttccatt
     3361 ttcttaatat catcaccatg tataaactta cctggtaaag tatcatgagc atgacctaat
     3421 tgctcttctg gaaaactaaa tatagttttt ccacttatag tatctaactc tccaactata
     3481 ccacatagag cacaaattgc ctttttagta cctggctcaa ggtagaataa tctattgtgg
     3541 caatgtggac atacaccttc ttctccttga tatgaagctt tttcaaaatc ttttgctgct
     3601 tcaactatat ttaaaccaat ttgatgaact tgaacaagcc ttgtatcatc cattactaat
     3661 tcctttgccc atgggaacca agcattatct ataactttcc acattggtat gagagctagc
     3721 atagcatgtt cacattgaac tcttgtaccc cagtcagaac ctcctattcc cataaatgat
     3781 attactttat cattaagcat tcttgaatct ggaatttttc ctcctataac ttctgcaact
     3841 tttgttccta taatgttatt tcctctatct gctctgggtc caaaacggtc cattatagta
     3901 tggaataatc ctgatgcccc ttcttcaaaa atagggtctg aaactactat accatctgca
     3961 tctttcattt tgtcaagcaa ccattcaaaa tcatctttta atacacacat actacctttc
     4021 ccagataata aagttttaac acacgcacaa catccagtac aatgtttaat gttaatagat
     4081 gacatttgaa taaattctac ttcagcccct gcttctttgg cacctttaag tgcaacttta
     4141 cacattgtgt cattatttcc attttttgtt ccaaaagaaa tacctagtat tttcattttc
     4201 aactctccct ctttagtatt tatttaatct tctttcattt ttaatatata gaatccatca
     4261 tctaaagcat ctatgatgat gtagcctcta tcatcaacga taagatcttc tgttactgcc
     4321 aagcgtggtc ctgggaatcc tgggaaataa gattcttctg gtgttttatt tggatttggt
     4381 ggtataaaat atgctagctc tttgatataa tatgggtctg atacatcata aacccttagc
     4441 cctgcatgga aataacagca atatactcta tctcctcttt gctctaacca tggcttatta
     4501 tccattggtt catgaagatt atgtggacca aatggccctg gttttcctaa tcctgcaaca
     4561 ttaaagttag gataagggaa atcttttgga acttcaggat atggaaattg agcaattaat
     4621 gttggttttg ttgggtcact aacatcaacc atatgtatat tattcatagc ttgaacatct
     4681 gtaatgttat ctggtttaaa gaactggaat ctttctccct cattttgaac aacgactaaa
     4741 tctcttcctg gcaatggtaa tgctgtatga gttcttgcac ctgcaagtct actagaaaat
     4801 gcaggcgtaa atggcaattc acctaagcat cttggtcttg ttaaatcttc aacatctaat
     4861 acaactaaac cagctcctcc ataaccacaa tatgcttttc cgtctcttac aaatggaggt
     4921 ccatgaagcc atcctttatc cataaattct gggcaatgag gtgctcctgc atcaaaagtt
     4981 ctatttggat atccatctgc atattggtct ggtctccacc atttacctat ttccactgga
     5041 ttagtaggat ttattatatc tatgacccta taaatcagac cttcaaagcc aacacaatca
     5101 cttgataaat gtacatatct tccaccattg tacatgaatc tatgaacacc cattacatgc
     5161 tttaagccac aatcccaata tcctaaaaac ttaggattta aagggtcttc ttttaaactg
     5221 tatattctaa ttcctgcttc acacttaata tttgctaatt tagcttggtc aacaagtgct
     5281 cctggtccac ttccacaact cattgctact atcattaaat catctgctat ttgaattttg
     5341 ggagttgttg ttgtaggata ctctttaggg tctaacattt gaaaatgttt tataaattgt
     5401 ggattataag ggtctgtaat atcgctaatc attactccat tttttttatt tcctccaaaa
     5461 cagcatccat ataaatagta tttttcatct cttttgtaca gagccatttg aaacattcca
     5521 caagttccat ctaagttatt aaaggaacaa acttcaagat ttttaatata tccgttattt
     5581 ccgttgcctt ttgcataaaa tttatcttcc attttttctc ccttcttatt ttaaagtatt
     5641 atcaattgat atactttcat tttatgagac aaacttactt aaatccaata tcgttttcat
     5701 tggaatatat aagttattct tattaataaa tttataaaat ctaatgtaaa gatatactta
     5761 aaacatatat ttttatacaa atattttcta atattcttta aaagtaaatt ttcttatact
     5821 ataataagct ttaattatag ataaataagg tttacaaaca atcaagttta aataggggtg
     5881 attatttgaa tactaaacaa ttagaatatt ttatatctgt agcagaaaag ctaagtttta
     5941 ccaaaacagc agaaaaattt tatatttctc aaactgctgt tacacagcaa ataaaagctt
     6001 tagaagaaca aataaatgta actctattta caagaagtaa acgtcatgtt gaactaacac
     6061 cagctggaaa agttttttta tctgaggcac gtactataat aaaaaatatc aatgatgcta
     6121 ttgcaaaaac acaacaattt gcacatggtt tttttggaac tttgagcatt ggaaccttaa
     6181 taggatatga aaaaaacaaa cttcaacaat atttaaaaga attttctaat acatacccta
     6241 atatatctat ggatatttgt actaatgaaa taaccgaact tttaaactta gtaaaaaata
     6301 attctatgga cctcgctttt gttattaatc cagaaaatca accattaaaa gattttgaat
     6361 ataaaactgt agaaagatat tctttggtag ctcttttacc tcctggtcat ccactttaca
     6421 atgaggattc tatcgattta attgaattac aacatgataa atttattttt gtaaaggaaa
     6481 caggggatga gtatggacaa aaatctatga tacaaaatag atatcgtgaa gcagggtttg
     6541 tacctaatgt ggtacaacgt tcaaataatc ttaatactat tgaatcattg gtatcttcta
     6601 acatgggaat atccatttta ccttcatttt gtgttacaga tacaatgata gaacgtgata
     6661 ttgcaatagt acctataaaa gaagtagaaa atagaattga agttgtagtt gtatggaata
     6721 aaaaaaatac aaatccagca ttagaaaagt tcatttctat aatataaaat taatcaatat
     6781 taacgataaa aatagcatta accatttttt ctatagttaa tgctattttc tactatttta
     6841 ttatttaact tttaatttaa tatttataat cattaaaaat aactctgttt aacaaatata
     6901 agcaagtcaa aaatgtgtct aaaaaagata aaaattttat tatatacaaa tgtattatct
     6961 caatatatct attttttcat tgttcatcag tctaagtaca tgacctgtgc ttactaaaga
     7021 attatcccca taagttgtat gagctaacac cgaactagca accccaaact caactatatc
     7081 ttcactcttc atattttcca aatatgaata tataattcct gctacatatc catctccggc
     7141 accaaccctg tcatatattt ctaacttata ctccctactt tcaaaataac catcatcatt
     7201 tgatatatat ccctttataa atctattatt catatcatca aatcttctca tagttccagc
     7261 tacaatacct atatcatact cttttttaat caattctgaa ctagatttta tatttagact
     7321 atctatatta agtatcttat ttatatctct ataactagca aacagcccat gacaatatgg
     7381 tagtattttc ttataatcat ttactaaatc atcataagtc ctttctggat ttaatgattg
     7441 tctaaaatta aaatcaaaga atacttttat ccccttttca taagattttc tagctagttc
     7501 taaagcaatc tctttaactg aatctgtaag ttgcatagca ataccacaaa tatggactat
     7561 atccattcct tctaaacatt tatcaatatc ataatcttgt aatttactct cacaaaatga
     7621 actatttaac ctattcaaat aagtcacttc agaaggtctt tctccatacc cattttcaag
     7681 aaaatacact cctatatgat tccctccata tgtgataaag tcatctttta ctccaagctt
     7741 tcttatattt gcacttaatg ctttacctac attattatta ggcaactttg tcaacatata
     7801 ggtattgtat ccaaagtttt taagtccaga taatatattt aatcccgtgc ctgtaattat
     7861 ataatttagt tcattggttt gtgttaagaa cttgtagtca ggtactgaaa gtctcatcat
     7921 tatttcacca aaagctaata tattcataaa tctatcccaa taattctttc attattttat
     7981 aaagtactcc tatatcttcc actttcgttt cacctgtttc tttatcaatt attgatgtat
     8041 aaacatgagg tataactttt tctacaccag catcaagtgc aatttgaact atctctttga
     8101 agttatctaa gtctatacct cctgttggtt ctaaataaaa attatgcttt gcacaagctt
     8161 ttgcaacttc tatatattca tcctttgttt taagtcctcc cattggaaaa aacttaattg
     8221 agcttcctcc catatctttc atcatagcta ttgctgtttc tataggaact ataccatctt
     8281 tttcttttga acttaatgga cctgtggata tttttacaaa tcctggttta ccagttggag
     8341 aaactaatcc atttattata ctatcatttt gaccaagtaa tgctctagac gtagccacac
     8401 ctgtaaacac ttgatttata tgttgtggct gaagttcttt agaaatctga gataccatat
     8461 ttgactgatt tgggtctcca gctccaagcc ccacagatat tgcattatct atttcctttg
     8521 agtatttttt catatcttca atagctgctt ctacagtact gtaattcttt gatagtactc
     8581 ctatcaatac atgcccgtca caagcatcat atacttcttt agcatttttt atgcttcctg
     8641 caagaacatt taaacaaact ctatttttat aatattgtgg tatacaattc atatttatcc
     8701 cctccattgg taaaatttaa tttattaaat cattataatg tcacttactc tacactctaa
     8761 ctttctacta tattttttat cctattataa atagtatcta aatcacttcc agttacactt
     8821 cttatatcaa tctcaatctt gccttcatta gccttatagt ctctagtgta tatagctatc
     8881 tctccacttc taagttcatt tataatttgt tgtgttgact tatttattat atcttcatta
     8941 aagtcaattt ctccacgaag tatttctcta cctgctgaat ccctagttat actagcactc
     9001 actcccttta tagtatttat cttgtctata aatggattta gcttgtcctc catttctttt
     9061 aaagttactt tatcattact tatatatctc tcaattgcac atgttagacc tacaatattt
     9121 tcttttccta ttttcatagc tcttcctata ccctttgatt gaagctttac attatctatg
     9181 tattttttct tacctattac taaccctgag ctaggaccct ctatagcctt agcaccactg
     9241 tatatcacta ggtctgctcc caattcataa tatacattta aatcttcttc tgctgctgca
     9301 tctactatca aaggtaaatt atattcttta gcaacttgta ctacatctaa tacacttagc
     9361 atactctttt gaacacaatg atgagattta atatacataa ttgctgctgt attttcattt
     9421 atctctctta ttatatgttc cttgttacac atattagcat aaccggcctc aacaacttct
     9481 cctcctccaa gtcttatcat tacttctact ggaactccat aatcaacatt atgtcccttt
     9541 ggaattatta tttccctttt aacttgtttc ttaggattat ttacattgta aactagactc
     9601 atatcatctt ttgctatcat tgctcccaca ctctgtgcaa tcccagcaga tgcactagaa
     9661 actattagtg cactttcaca ccctaataaa tttgctatat attcacctgt tttatttata
     9721 agttcttcta ttataaagaa atttttacac ccttcaccta ttgcttcaat aacttcctta
     9781 tctactgttg atactcctag tatagtcatt ttgcctgaag catttataac acgtcttaca
     9841 gagtattttt caaatatact actcatacaa tagtttctcc cttctactca ttctcttcta
     9901 tagacttacc tatatttaaa tattctccat tgactactac tgctcttggc tttatagacg
     9961 tacttgtgat tactgactta ttcaaagaat cttgaagttc tttttcttca ttaaccacat
    10021 caaatatagt taaatcagca tcatatcctt cttttattaa acctttattt tttaaactta
    10081 ttgcatctgc tgcattttta gtaactttat ttattatatc ttcaagtgaa tagcccatat
    10141 atataaactt ttccattgtt gtagatagat tatacactgg accatttatt ctatttttaa
    10201 tatatatatc tgtacttata gtgtctggaa aaattcctgc atccttagat ttcattgcaa
    10261 tatccatact aaaactttcg gttccatgac ctacatcaag gattatacct ctttctctag
    10321 cttctatgat ttctttttta acctcattgc ctctaagtat tccatttggc tttccattat
    10381 atatatgggt taatatatct cctttttcca tatagtcaaa tatatcctct atcgttggag
    10441 gagaactccc aaagtgaacc atcataggta gattaagttc attttttatt cttttagcta
    10501 cttttaaagg ttcaacatca tttgaaatta caacactttt actcattcta gctttgattc
    10561 caacaacaaa atctttgtat tttttaactg ctcttttaag ttcatattca tcaatattca
    10621 tcatacttga aagttcatct tgagatgtta ttccctgctt agcaatattt attaaagcat
    10681 atacatttgt tttatatttt ttagtatctt cataaaattt acctatatct aaagctcctg
    10741 ttgttcctgc atctactaca gtagtaactc cactttttat tccaacctca tctggataat
    10801 ccttataaag gtctagtctt tcgtagcaat gcgtatgtat atctatccaa ccagcactaa
    10861 taaatttctc tccatctaag tttataattt gttttgcctg atacttagaa tcatctatct
    10921 gattttttac ttctattatt ttatcgtcta atatagctat atcaattata cttttatcta
    10981 ctgtttttcc acattttatt aatatgtcta tcttcacttt actctccctc taaaataatc
    11041 ttaaatttac tccaaggttc aataaagtct aaaagtatta tttcgtcttc ctttattcta
    11101 cccaccttat tttttctttc atctgtacaa tcttgaagta tcaactgtaa ttccccttta
    11161 tactttccaa agtcattatt accaatcact acatctcctc ttttaaagag gttcttatta
    11221 tcatgtttag ggaaattttc tttagcatat ttttttctta cttctgtaga tcttgctacc
    11281 tgattagtta tatctcctct tctaaagtgt tgctcattta ccattattat cttttctact
    11341 tcagaagttt tttcttcagc tactacatta aatacaactt ggtattcatt tacttcagac
    11401 attcttttta attcatcttc acttgcatat gcattcccta caattacatc atcaatcaat
    11461 cctgttgcga ataagtgctt agttgctgta gcaatatcta agtttctatg catttcaagc
    11521 gtacacagcc catcatttat attccaaggc cctattgttg cgtattttga attaacaaaa
    11581 gcagcagttc ttattccata ctttttaaat ctatttgaac atcttacaaa aaaatcatat
    11641 ggcaagcctg tacccctttg aggataaaaa ttgtgacaac catatatatt atttacattt
    11701 ggcttgtaag taattatatt atccaagtat gctacatcat tactcatatt tagttccact
    11761 tttaaatcat atggattgaa agaaatcata gcttctttac ttccatcata tccatagtca
    11821 agccttattc cactagcatt taactcacta aaaaatgtta aatcatcata agatatttgt
    11881 aatatatcaa atatgtttgg agctatatca aggattactt caaatcctaa ttctcttgca
    11941 taggttataa tttctttaaa atgtttttta acttcttcct taggtcttgt aattgaaagc
    12001 atgcacataa atattctttt aaatccgtat tttgaagcca tttttatata tgccttatca
    12061 tcttccatgt tactatgctc tggatatact gatattccta atcttctttc catctttctc
    12121 ctcctcaaat tacattattt aaaatatata aaattaaagg caaaggtcat tcctcctacc
    12181 ttcaccctta ataacttata agctttttct atattttata tagtacaaaa cctctatatc
    12241 tatgtatttt tataaatttt cactcatttc ttcttgttct ctttgtaatt gttgtttttc
    12301 aaaaactttg aagaatggat aataaacagc aaatgatatc acaaagttga ttaatactaa
    12361 gatacctgct agtatactcc agttagtact cattactgct gctattggtg ctggcattgc
    12421 aaatggtaat cttgctacca tcataggtat aacccctgta atagtaagaa catatgatag
    12481 aattgtcatt attataggtc caattataaa tggtatccct agtattggat tcataactat
    12541 tggtgctcca aatattatag gttcatttat gttgaataac cctggtaaaa atcctaatct
    12601 acctaaactt tttagatatt gtgacttaga aaacataaac attacaacta atgcaagagt
    12661 agcccctgct ccccctatcc atacatacca ctgtaaaaat tgttctgtaa atatgtttgg
    12721 cattgcatga gctgatactc cttctgtgaa tgcagttata ttttctgcta tagacatgtc
    12781 ccatataggt cttattactg gccccattat agctggacca tgtattccaa gtacccagaa
    12841 taaagttatt aggaatactg tcagtaaccc accacctaaa ctatttcctg ataagaatcc
    12901 ttttaaaggt gataacatag cgcttagtgc aacacttata tcaaatccta aaatatgtct
    12961 tattacccag aataacatta aaattactgc tgtcggtaat aatgcagtaa atgaatttga
    13021 aacctctact ggaactcctg ctggcatctt aattgtaata ttcttttcct tcataaaacg
    13081 atatatttca actgtaatta acgatgttac gatagctcca aataatgatg gcgaattaag
    13141 ttttgccata tccatccatc taccagcttc tataactcca cctactggtt caactacttg
    13201 agttggaact attgtagaaa ctaaaaatgc cataactgca agcataccga cacctgttgt
    13261 atctaaatcg tagcttttag caagtgatga tgctatccca aaagtagcat atagagataa
    13321 aaatcctaca gtatatctaa atggtatatc taatattgct ttaaatggag ctataaaatc
    13381 catataagca tcaataggta tgtttaataa tattgtgaaa aatgacccca ctattgttag
    13441 tggtaatgtt gctattatac ctgctcttac agctctcata tgtctctgat tacttatctt
    13501 cgctgctaca ggcataatct tgcgctctaa aaaacttgcg aatttttcca tagttatccc
    13561 cctgtattat tgatattatc atttatttat aatgcaataa cgatttcata acataaacta
    13621 atttttatgt aattgataat atcacttatt tattaaaaat attataacat tataatgttt
    13681 ggttgtaaac attataatgt tataatattt ttttgttttt acaactaaac aaaaatcctg
    13741 agcatattat catttcaata aactcaggat tc
//
LOCUS       DAGSHT010000085        13768 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGSHT010000085 DAGSHT010000000
VERSION     DAGSHT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.087"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1058)
                     /locus_tag="MJB72_002168"
     CDS             complement(<1..1058)
                     /locus_tag="MJB72_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBY8263274.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLV"
     gene            complement(1439..4774)
                     /locus_tag="MJB72_002169"
     CDS             complement(1439..4774)
                     /locus_tag="MJB72_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBY8263275.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGQEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(4823..5251)
                     /locus_tag="MJB72_002170"
     CDS             complement(4823..5251)
                     /locus_tag="MJB72_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263276.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(5241..5621)
                     /locus_tag="MJB72_002171"
     CDS             complement(5241..5621)
                     /locus_tag="MJB72_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBY8263277.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(5654..5962)
                     /locus_tag="MJB72_002172"
     CDS             complement(5654..5962)
                     /locus_tag="MJB72_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263278.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(5995..7179)
                     /locus_tag="MJB72_002173"
     CDS             complement(5995..7179)
                     /locus_tag="MJB72_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBY8263279.1"
                     /translation="MVDEAKILDVVSISGENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(7183..8645)
                     /locus_tag="MJB72_002174"
                     /pseudo
     CDS             complement(7183..8645)
                     /locus_tag="MJB72_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
     gene            complement(9155..10024)
                     /gene="gatY"
                     /locus_tag="MJB72_002175"
     CDS             complement(9155..10024)
                     /gene="gatY"
                     /locus_tag="MJB72_002175"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBY8263280.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(10044..11960)
                     /locus_tag="MJB72_002176"
     CDS             complement(10044..11960)
                     /locus_tag="MJB72_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBY8263281.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(11963..12883)
                     /locus_tag="MJB72_002177"
     CDS             complement(11963..12883)
                     /locus_tag="MJB72_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBY8263282.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(12985..13737)
                     /locus_tag="MJB72_002178"
     CDS             complement(12985..13737)
                     /locus_tag="MJB72_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8263283.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
BASE COUNT         4260 a         2631 c         1364 g         5513 t
ORIGIN      
        1 tctactaaat taagggattc tacaagtact tttttattta ttataccttc attataatca
       61 tcaaataatt ctaatataaa cggatatgat acaaatattt caagtttgtt tatccttttc
      121 aaaactttgt ttacatcatt atcatcagta catgaataag atatctctct ataatagtta
      181 gaaaatttaa gtaaatcttt taataaaatt tctatatcta taccattatc tgatacatac
      241 cttttaaaac taaaatatac tttattttta ttcggtatac ttctctcctt tatagtcaaa
      301 tagtctctta taaaactact tacgttgcta cttgtatttt tttcaatttt ataccaataa
      361 ttattataaa attcctcttg taacttagag cttctaccca ttaacacaaa gtttcttact
      421 ctatcagctt cacttaaatc taagccagtt gaatttagac tttcaaagat taattgaggg
      481 tcatcttcac cattttttaa ctctatttct actatagtta atttctgtat agcatcaaac
      541 aaatcatcta ttgatatatt atcttctttt attctattat aaaaatataa ataatttgaa
      601 actatattag aatttctatc tgtattcatt tcttcttcaa atagttcata gtacgctttt
      661 ctatcttctg ctattggttt taactttatt tttccatctt tatctcttga gtgcttgttt
      721 ataagataac actccttaat ctgttcttct gttgtaataa ttccttttga ttccccaaac
      781 tcttttatta tttgacacat ggcaatcaat aatattgaca cagtagtcac tctttgttgt
      841 ccatctatta ttaaatattc ttttcccata ctatatatag atacaactga ccctaagaaa
      901 tgagttctaa atccagaatc tattatatcc tctaaatcat caaacaatct tttacattgt
      961 tctttcttcc agttataatt tctttgatat acaggtacta caaaactttt gtttgacccc
     1021 tctaaataac taagaaattc aatttctcct gctttcatat taactccccc tataagttaa
     1081 ataaattcca ttcataactc catgtatcta tatattatat caaaattttg actcataaat
     1141 gtatatgaaa gccttattat ataccttttt atacctgttt ttattttatt aaagtaaata
     1201 catgattatt ctataaaata tacaaatact taattttaaa aattattatt taatcttctt
     1261 catgagtaat tcatagttat ttatcaatta aaaagagata ttgcataatt attcaatacc
     1321 tctctttgta tatttacatt atccatatct agttatctat ttcaacccaa tattagacta
     1381 cctactaata aatttattct ctacatcatt aacaataatt ttgcactaaa taacatcatt
     1441 agcctatctc tgatgcattt tctttaattt tatttatagt tttaatcaat ttatttctct
     1501 cttcaatatg attttcaaat aaatcaatta tacttccaac agttctaaat gggtcttcca
     1561 tcaacacttt gttatcctct ataaatccat tcttgactac ataatcgact ataagtttaa
     1621 caaaatgtat ttgcttagta ttaaagctct tattatttaa aaactctgaa aaagcttcat
     1681 ttgcagcttc tctatccaat cccactagtc ttctcactaa ttggcttact ggagtatctc
     1741 caaactcctt cttataatca tcacaattac ctaactgttt taagagtatt tcctccaacg
     1801 tttcaacatc ttgctttgtt atctttttat tatttcttaa tttaaatatt gctaattcat
     1861 ctttatggct atttaaataa tactctacct tctttttata attcttaaga ttgtttgcat
     1921 tatatacaga atcatttcta tcttctgcta caatcatatc ttcaaaactt gtataatata
     1981 tcttttgact agacttttca agatacttaa taagctctct taatgatata cgtacttctt
     2041 ctacctcaaa tatatctgaa tcttcccaaa aatcctctgt cattgctttt tcaatagtat
     2101 atttttgttg ctgaatctgt ggtatagtcc caagttttga aagtttttct gctgtagcca
     2161 ttacatgcct tataccttta cttgcattat tattttgtaa gtatgcaagt tgtatattat
     2221 acataactac atcaaatctt ttagccaatt catcatcact atatgaagtt atgagtggtg
     2281 atatatattc tcttatctca ttttgtgcta ctgctccaat attgctccaa acattttcat
     2341 ttttatactt atgtacataa cttaaattca tcctaactat ataactatcc tcatttaaag
     2401 cttttacaga agttataagt tcttctaaca attcttttct aagttctgca tactcacctt
     2461 ctatatattt aatatcttga agttctttaa ccatacttac ctttatatta aataattttt
     2521 cagtaagtgt ttctgcttta tttccctcaa atcctttagg attaaattta aaaaattcaa
     2581 aattattaca aaaatcaaat attaagaatc tctccttatc catatcaaca cctaataaat
     2641 ctggacataa tctagttcct ctacctatca tttgccaaaa cttagtctta cttctcactt
     2701 tcttgaaaaa aacaagattt agtatttcag gtatgtcaat tcctgtatca agcatgtcta
     2761 ctgaaactgc tatttgaggt aatttattct tatcagaaaa atcatctatt atagaatcta
     2821 cataatttat actatagtca actgctttta caaagttccc accatacttt ggatacagct
     2881 tattaaatct ttcaactatt gccctagcat gctttgtatt ctttgcaaaa attatagtct
     2941 taccaatctt ttcttgtcct tctattttca acccctctgt cattagctta tttaaaacta
     3001 aatctattgt gtcagaatta aatagccact catttactgc attattccct atttcgtctc
     3061 caatactctc atcatcatta aatgtatttt catattcttc cttctcttca tcagaaagct
     3121 catcatactt aattccatct tccataatct tagtcttaaa ctcaatacta gtataatcta
     3181 ctaaatatcc atcctcgact gccttattat actcataagc atatgtaggt actccatttt
     3241 ccatgtcaaa aactgaataa gtatttttat ctatctcatc ctttggtgta gcagtaagac
     3301 ccattaagta agaatcaaaa taatcaaaga ttgacttata ctttttataa atacttctat
     3361 gcgattcatc tattataatt aaatcaaaat gaccaggtgt aaacaatctc tttccatcct
     3421 tagacttcgt atcatcaatt gcattcatca ttgttggata tgtagaaaat atcatcctag
     3481 catcttctgg gctatctttg ctatccaata aattacataa cgccaagtta ggtaatagct
     3541 tagtaaaact ttttttagct tgctttacca atgcttttct atctgctaga aataatatat
     3601 ttttaatcca attatgtctt gaaagtacat caactataga tatagcaatt cttgtttttc
     3661 ctgtaccagt agcacatacc aaaagagttt tgcgttgctt attttcaaga gcctcacata
     3721 cagccctaat agcttctttt tgataatatc tattggatat ttcatcattt attgttacag
     3781 aagttaaaga cttcttactc tttcttctat ctatcataag ttgtaattct gattttttat
     3841 aaaatccata aactttccta tctgaatagt catcccaaat atacatatca aaaccattag
     3901 tcaaaaatat taccggtctt tgactatatc tcttttccaa acaatctgca tataatttag
     3961 cttgttgttg accaattttc gggtctttac tcgttctttt cgcctcaact actgctaatg
     4021 gcttaccatt ttcaccataa agaacataat ctacatatcc tacaccactt tcattaggca
     4081 taccttgtac ttctatttct tcccatatat cttttccaaa ttcccaacct gcaagcttta
     4141 attctatatt tatatatctt tttcttgtct caaattcgct tacttcgtct atcttgaaat
     4201 catagtttgc agtattaact tttctctttt cagttagtga ctttcttaat tcctcatttt
     4261 cttttattac ttcttcaagc tttttatctt ttgaacttaa tctctcataa agatttttaa
     4321 gttcgtctgg tctcgtcctt ttctcttccc catgaagtaa taaatcttca ttaaagtctt
     4381 ctgctgtata ttcatctgaa taacaatagt ctatccaact aacaaattga tgcaaattgt
     4441 taagtgaaag tattgcttct cctctctcta tgttcgcact agtatgtaca gataaattac
     4501 ctaatttaac tatatatttt aataaaggta acatatcata gtctataagt tctataaaac
     4561 tattattatg aatcaacgta ctgagattat cttgataagg caatactaaa tcactatcat
     4621 tagcatatag ccattttact gcaagctcta aagctcttct tgttatagtt gcacaagtag
     4681 atggactaac tagtatactt ttttctgctt ctaagcaagc agtattaaaa ctattgaaat
     4741 ttttcttctt ttgtaaaaaa tcaaaattac ccaagctttt atcccccttt tgtcccttag
     4801 ttaaaataaa tctctctaaa aactatttct tacagctgtt tcttatacta acaaatactt
     4861 cttcattttc tgtaaacaat tcgtacatat cttcaaactg tggtatatca tatccatgag
     4921 aatcatcatt tctcaatttt ctaagtgttt ctataactat agaagaattt ttaggtaata
     4981 aattgtgata ctcacaagct aaaaggtagt cataaaagtt tttagatttt atgtttaaat
     5041 caattgattt ataatacaac attgcttttg ttaattctag aaatgatacc atatagcttt
     5101 taaagccttc ttttatgtat ctagcccata actcaggcat ttcttcctta tcatataaga
     5161 ttttgaagtt ttgcaggtca tacaagcaag aaccaaaatc cctatttact tcttctattg
     5221 agttccaaga taatttgttt ctaattacca tacatgtccc tccaatctaa gatatctatt
     5281 acatcactgt tttcaaaata gaatttattt atataatcct taaaatcttt agattcataa
     5341 ataacttcac cttctaaaat agctttttta agtgctattg tataattatc tgtaacaatt
     5401 tttaggtcta catcaatgtc taatatattt cttagagctt ctgtcttagt tgcaagttct
     5461 gtaattggta cttttttgat aggaatccaa gctatgtcta tatcactttc tgatgtatgt
     5521 ttatctgttc cataactacc aaatattatg attgctagag tattttcttt tggaaataca
     5581 tttattattg agtttttaat ctcatttggt atatttctca tttaatcacc tcttgtttat
     5641 taggtaatat tacttaatta aatttatatc cataatcttt taatctattt atacatattt
     5701 ctctttcttc atcagtaaac aactctttat acttttcttt taatactaat acttctaatg
     5761 ataaatctaa tctcttacac tcccatagtc ttgtaaatcc ttcagtagct ccttctttat
     5821 tgattaattc tttagcagct aataaggctc ctttgttatt tatcagttgt aaaaatcttg
     5881 ttggcttata gttacattgt aaatcagctt ctttatagat atttaccatg ttatggtata
     5941 aatctagctc tatttgattc atagatttta ttcccccatt ttaataattg tattttagtt
     6001 gaataactct cctttaaaag ctttttgcat taatgagtta aagttgtctt ctagttcttt
     6061 taagctagtt tccatttcaa acttcaaaga gtttacttgt ttaacgaagt tggcaaattg
     6121 attttgaagt ctaataggag gtacaataat atttattttt ctaacagcat ctttagttat
     6181 cgtttttgtt acagctcctc ttactttctc ttttatttta ctttccatat aggatgttag
     6241 taaaaggtta tataagaata tatgattcac tttatcttca ttaaatctaa gcataaattg
     6301 attcatagct aaagatactg gtttatttaa aaatggcatt aaataaactt tacctgcact
     6361 acctatctta tttattataa cctcaccacc aaacaccttt gatttttcaa gataattata
     6421 tgcatgttca tcgatatact taacaccttt ttcaaagtta ttattttcta aatctgtagt
     6481 tcttaccatt aaggcataac cttttgtatc taaaagagtt acattatctc ttaaggtttc
     6541 ataactacca tttgaatgat aatcagttaa aacatctaaa tatttcccta tttctgaaat
     6601 ttcccagttt ttttcattct ttgatggagt accaaacatc tcaataaatc ttgatttaac
     6661 taattcatcc aatacttcta tttgctcttt tcttttatct attaactctt gagccttgtc
     6721 taaaacacgc actacctttt cttgcacttt taaattatac atgcgtattt ttatattagc
     6781 taatccttca ttatttaaag ctgattgtgt agcaccttta cttaacctat ttttttgatt
     6841 tagaaaataa ctactattta agtaatggta taaatattga ggcaatattt cttttgatgg
     6901 ttttaaaaca taaaatccag tcgatactat tatattttta ttagttttat taataactaa
     6961 tgttttcttt gtgtccttca tttttgcaaa tataacttct cctatattta cagtttgatt
     7021 tgctctagaa ggtttatttt cataagtaac tatttctaga ttgcttatct tgttaaaatc
     7081 taagtttcca gtactaagat atgagcgttc tccatcaaac ttttttacat tttccccgct
     7141 tatactaaca acatctaaaa tttttgcttc atcaaccata ctctacacct ctaacattct
     7201 ttctagttcc tctaatccat ccactatctc tttctctaac gccttaccct acccataata
     7261 acactaggag catcataaac tacttcttca tacactacct ctttatactt attaatagat
     7321 aaatcatacc cattctctct tatctcttcc acatcaacaa aaaaactttg ctctgttctt
     7381 tttctatcta cttcaccatc aagattatca aatctcttaa taatatcctc tatatcacta
     7441 tcctttatct catttctctt atcatccaaa gaataaccat cagctttcat atcatagaac
     7501 cataccttat cagttccacc tgttcctgtt ttagtaaata tcattatagc tgtagatact
     7561 cctgcataag gcttaaacac tccacttggc attgatatta tagcttccaa tttatgatta
     7621 tcaactattt cttttcttat cgatttatgt ccacctgttg aaccaaacaa tactccatct
     7681 ggcactatac atgcgcatct tccaccaatt tttaaaactc ttaaaaaaag tgccaagaat
     7741 aataactctg ttttctttgt cttagtcact tttaataaat ctgctgacac tgcttcataa
     7801 tctaaacttc ccttaaaagg aggatttgct aacaccaatg taaacttatc tttatctgta
     7861 ttaacctccg ataaagaatc cttatactct atattaggat tgtcaacgcc atgcaacatc
     7921 atattcatag cacctattct aagcatagtt ctatccatat caaatccata gaacatacca
     7981 ttattaaaat gctccttcag cccttgcact aagaaaagac ttgaatgatt atcccttaag
     8041 tattgttgtg atgatactaa aaaacctgct gaacctgctg ccgggtctac tataatatct
     8101 tctggtgttg gtttaataag tcttactatc atatctatta tgtgtcttgg agtcctaaat
     8161 tgaccattag tacctgctgt agccactttg gataataaat actcatacaa atctcccttt
     8221 gtatctctat ccttacttaa ttctaaatta ctaataccat caactatttt agataacata
     8281 agaggcgtag gaattttaaa tattgcatct cccatgtact ttgcataagc tgagtcttta
     8341 tttccatgta atttttttat gaaaggaaac acttcatttt gtactatttc atacattttt
     8401 tctgaatttc cttcattaga aaacttactc catcttaaat gttgatgttc atcattaaat
     8461 atcctttcaa aagaaactcc caaaagagtt gcttctgctt cattttttgt ttctacttca
     8521 tccaatcctt taataaataa aagatatgta aattgttcta taacttctaa tggattagtt
     8581 aatccaccag tccaaaatgt ctcccaaatc ttgtctactt tgctccttaa ttctcctgta
     8641 atcatatatg actttccctc cagtgttcat aaaataattt aaatatatat tttagtcgta
     8701 cgccttaata gttagtgtct tatgttaaat ccaagttttt actacacagt tattaaatta
     8761 tttttcctat ttacatgttt tagttaaaaa actaatgcaa tactcttatt ttatgattat
     8821 tatatatacc atacaaaatt attttatcat atatccatca tagaagtcat ctacctggat
     8881 aattcttacc atattataac tttttaatat taatttaaac ttagaaagaa cttttttatt
     8941 taattatatc atcttcctta tttcaaattt ctcaattaca atattaatca aaaaataaaa
     9001 taagatgctg tctcaaaata gatataatct tcttttttat tgtcagaatg atgaaaatat
     9061 atttaatttc atacaaaaaa gagtgcctca tgaactaaaa agttcatttt gagacacccc
     9121 ttaatttaat cttacttatt ctttcccttt attattaaca cttcactgtc tctattgctt
     9181 ttcctacaca tccacaaact tccatcctac tcttaacaaa atctttaact gactctaaac
     9241 cttttttacc atatttctta gggtcaatta cctctgggtc agaattaagc acattcttaa
     9301 ctccattgct ataagcaatc cttaattcag ttgcataatt aaccttgcaa atccctcttt
     9361 ttatagattc cttaataact tcatctggta ttccagaccc accatgtaat actaatggaa
     9421 ctgatacaac atctctaatt tcagataatc tatctaaatc aagctttggc tctcccttat
     9481 ataatccatg tgccgtacct attgctactg ctaaagatga tacccctgtt ctctctacaa
     9541 actcttttgc ttctaatggg tctgtatatg ctcccccatc accaccatct aaatcgtctt
     9601 ccttacctcc aactttacct aattcagctt caacaggtat atctgaaggc atacatgcat
     9661 ctacaacaga tttagatatt gaaatattat cttcataact ttcatgagaa ccatcaatca
     9721 taatagaagt atatccaact ctaagtgcct gcatagctag tccaaagctt gaaccatggt
     9781 ctaagtgcat tgccacagga acacttgcct tctctgatgc tacctttaca ttcgctaaat
     9841 aataatctag tcctgcatac tttattgttg aaggtgttgt ttgcaatatt actggtgatt
     9901 taagttcttc agcagcctct attactgcca taaccatctc catattttca acattaaatg
     9961 cacctactgc ataatgccct tcttgtgcat ctaataatat ttgtttagtt gtcactaatg
    10021 ccataatttc tcctcctata tgtttatact gatttaattt cttcgttatt taattttgat
    10081 actatttctc tacttgtttt gctatttaat aactcattta aaaattcttc attcattaat
    10141 tttgaagata gttgagataa tgtttttaag tgtaaattat caccattatc tgtagctgct
    10201 atcataaata taatatgtgc caagcttcca tcttctgatt caaagtcaaa cccttctttt
    10261 gatacaccta ctgctacacg tggtaccttt actgcatctg tttttgcatg tggtatcgct
    10321 atccccatac caaaacctgt agatgatatt tcttctcttt taaggatttc ttctttaaac
    10381 ttctttttat ctaaaagaac accagattta tcaagtattc caatcatttc atcaattaca
    10441 tctgttttat tttttccctt aacatctaaa attactgttt cttcgctata taaagagata
    10501 ttattagata cgttttccga ttctgcactt tcatttttat gttcatcttt tgttgcattt
    10561 gtattttcta tagtattttt ctcatttact actttaggtg taaccatcat ataaagtaca
    10621 gcacctacta atgaacctac aaatatacac cctacccata aaagtggctt attgactaat
    10681 cctagaacca aaaaccctcc atgtggagct ggtacttcta ttttcattat ataagttaaa
    10741 attgatgata ttgatgatgc aatcataagt actggcaaca cttttaatgg attttttgct
    10801 gcaaaaggta ttgccccctc tgttatatga gtactaccta gtatataatt gattaatcct
    10861 gctgctctat cttcttctgt aaatctattt ttaaataaag tagatgctat tgctattacc
    10921 aatggtggtg ttatacaagc tgcagataca cctgccataa ataaataatt tccctgtcct
    10981 agaagcatag ttccagttac atatgctgct ttatttactg gacctcccat atcaaatgct
    11041 gacatacatc ctattactat tcccaaaatt ataggacttg aattttgtaa ccccgataga
    11101 aagttcatca tagcattgtt aagtgctgaa catggcccac caagtaacaa cattgctaca
    11161 cccacaagac ctacgctgac aataggaatt ataaaaatcg atttaagacc ttctaattgt
    11221 cgtggtaatt ttcttaacaa atgtacaaat agtttaacta agtaacctgc taaaaatcct
    11281 gatatgattc cacctaaaaa ccccgaacct gtatcaaatg atatcattcc tccaacaagt
    11341 cctgccaaca taccagctct tcccgatata gattgagcta taaatgctgc caatacagga
    11401 accatcaagt tcatagaata tccaccaaca gattttaatg tagctgctat ttgattatat
    11461 tgttctgaat ttgggtcaaa tgaataaatc ccccataaaa atgatatagc agtaagtact
    11521 ccacctgcta ctactaaagg taacatgtga gatacaccat tcattaaatc tttatacaac
    11581 ttttgaccaa acgataagtt tccttcttct gcaattttac tgcttgataa gtttcctttt
    11641 ctaatagcta cttttccatc aatacatctt tgtataagtt cattagcttt atgtatacct
    11701 tctttgacag gtacttctat tactggcttt ccattgaatc tgtccatatc aacattttta
    11761 tcacaagcaa ctattattgc atctgccatt tcgatatctt ttgctgttaa cttattttct
    11821 acacctatag ctccattcgt ttcaactttt atgtcacacc ctaattccct ggctgaattt
    11881 tttaatgctt cttctgccat aaaagtatga gctattccag ttggacaacc tgttacccct
    11941 agaatcattt tctttcccat ttttatacca cctttctgac tcttatattt tttatataat
    12001 cttccacatt ctccaaatta cctataccat tactttcagc cacacttgct cctgttgctg
    12061 ctgacctttt taatgcttct tcaatatgtt ctggattttc caaccatata cttaaaaaag
    12121 cagctaatgc agaatcacca gcacatgcag agctgactac tgtaactggt tgagcactag
    12181 cataataaat tgattttcca ttataaaaat aagaaccttt ttctcctaaa gttagcaata
    12241 tattttgagc accttttaaa tgtagtaatt taagtacatc cttaatatct tcttcatctc
    12301 ttacaataat tccaaatata tctttgattt cttcatcatt tggttttatc aaatatggat
    12361 tatactgtag taactccttt aattttggtg aacttatatc gagtattgtc tttatacttt
    12421 tattttcaca aattttaaat atatcctcat agtaattatc atcaatacca ggaggtagac
    12481 ttccacttat tgataagtaa cttatatctt ctaatgactg tatcttatct agcatatcta
    12541 gtttttgttt ttcgtttaca tatgacccag agtttacaaa tttaaattca tcacttccat
    12601 catttaaaaa tatatttata cgagttgtat cttctaccca agtaggtaat acgtcaaatc
    12661 ctctcttttc gctttcttca actatatatt ttcctgaaaa tcctccaaaa aaccctaata
    12721 tcttagattc tataccataa tgacctagta caaaagttac atttaatccc tttccattag
    12781 gagaatatac agcatttgat gtacgattta ctaattttct ctttattcca ttagtagata
    12841 tattcatatc tattgcagga ttcgtagtta atgtatatat cattgtcttc ccccttgctc
    12901 gtgctgtgta tgttttcatt ataataaaaa agtctatgaa aatcttttca caaatagaaa
    12961 atcgattttc atagactttt caatttaatt ttcagaatta ttatatctga ccatataatc
    13021 tataattatc ctagatataa tatacaaggg caatctggaa gatacttcat attcagaaat
    13081 tgatatatgt tcatgtttgt atcctacaat atataaatcc gaatacttta taagttttgc
    13141 actttcacta caacagcaag ttataacctt agctccaact aaattggcat tctgtgctga
    13201 ttctacaagc tctatggtat ttccattcaa tgaaaatata attataagtt cgtctttttt
    13261 tattgattta gattttattt tcattatgtt ggggtcattt ataaaaacaa cattaaaatc
    13321 aagtaattgc aatttaagac taaactcttt tgctacatat tcagtaagcc ctcttgctaa
    13381 aatatatatc ttactagatt tatttatagt ttttattata ttgattagtg tagttaaaga
    13441 tatttgttct ataactctct gtgcttctat aaaggattta tttattatat cccccatatt
    13501 ttgtatatca tcattttcat cttttactgt taatctatac ctaagttcat tgaatccatt
    13561 taaaccgcat tttctaattg ctcttgaaac ggttgctgga gatgaatatg tttcatgagc
    13621 aatctctact atagataatt ctggtaatat tgattcattt tcatttataa atttaattat
    13681 ttcaagttcc gtcttcgtta gattttcaga attacttttg tttagctcaa ttatcatatg
    13741 ttatctcccc ttatataagt tttatttg
//
LOCUS       DAGSHT010000086        13749 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGSHT010000086 DAGSHT010000000
VERSION     DAGSHT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.088"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..199)
                     /locus_tag="MJB72_002179"
     CDS             complement(<1..199)
                     /locus_tag="MJB72_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBY8263284.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTG"
     gene            complement(229..1494)
                     /locus_tag="MJB72_002180"
     CDS             complement(229..1494)
                     /locus_tag="MJB72_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBY8263285.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(1604..1706)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1760..4417)
                     /locus_tag="MJB72_002181"
     CDS             complement(1760..4417)
                     /locus_tag="MJB72_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBY8263286.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(4545..5288)
                     /gene="yaaA"
                     /locus_tag="MJB72_002182"
     CDS             complement(4545..5288)
                     /gene="yaaA"
                     /locus_tag="MJB72_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBY8263287.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(5298..5762)
                     /gene="bcp"
                     /locus_tag="MJB72_002183"
     CDS             complement(5298..5762)
                     /gene="bcp"
                     /locus_tag="MJB72_002183"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBY8263288.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(6114..7811)
                     /gene="ade"
                     /locus_tag="MJB72_002184"
     CDS             complement(6114..7811)
                     /gene="ade"
                     /locus_tag="MJB72_002184"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBY8263289.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(8117..9271)
                     /locus_tag="MJB72_002185"
     CDS             complement(8117..9271)
                     /locus_tag="MJB72_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBY8263290.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(9516..10355)
                     /locus_tag="MJB72_002186"
     CDS             complement(9516..10355)
                     /locus_tag="MJB72_002186"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBY8263291.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(10368..10964)
                     /locus_tag="MJB72_002187"
     CDS             complement(10368..10964)
                     /locus_tag="MJB72_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBY8263292.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(11015..11665)
                     /locus_tag="MJB72_002188"
     CDS             complement(11015..11665)
                     /locus_tag="MJB72_002188"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBY8263293.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(11711..12964)
                     /locus_tag="MJB72_002189"
     CDS             complement(11711..12964)
                     /locus_tag="MJB72_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY8263294.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(12988..13629)
                     /locus_tag="MJB72_002190"
     CDS             complement(12988..13629)
                     /locus_tag="MJB72_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBY8263295.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
BASE COUNT         4294 a         2492 c         1495 g         5468 t
ORIGIN      
        1 atcctgtttt atctatcctt cttagtaaat ctaattctgt atctatcttt ataggcatta
       61 gatttagtat agctatctta gtgtctatgt tttcttcata ctttaattcc tttttaaaat
      121 cttctttata aattacatct attccttcat ctaataattt attaataaca ggtaatccct
      181 ttggtagtat taaagccata tcttttcact tctctttctc ttagaatatt agaaatttaa
      241 agcttgattt aaatcagcta ttaaatcctc tacattttct attccaacag ataatcttat
      301 tagtgaaggc aatactccag atgcaattaa ttgttcttct gttaattgtc tatgagtagt
      361 tgaagctgga tgtataacac aagttcttac atctgaaaca tgtgtaacca aagatgctat
      421 ctgtaatttt tccacaaatt ttttggcatt ttctaaccca cctcttactc caaatgataa
      481 aacaccacta catcctcttg ataaatactt tttggcattc tcataatact tgttatcttc
      541 aagtcctggg taattaatcc aatctacatt ttcatgtttt tctaaaaatc tagctatttt
      601 aagtgcattt tcactatgtc tctccattct aagatgtagt gtttcaacat ttaaattagt
      661 aagatacgca ttgaatgggc ttaaacaatt tccatagtct ctaagcaact gaactcttgc
      721 tttagttgca tatgcggcat ttccaaattt ttgagtatag cttataccat gatatgttgg
      781 gtctggctca actaattctg gaaattttcc attatcccag ttaaagtttc caccatccac
      841 tataattcct ccaacacttg aagcatgccc atctaaatat tttgtggtag aatgagtaac
      901 tatatttgct ccatacttta aagggttaca aagcactgga gatgctaatg aattatcaac
      961 aataaatgga acattaattc tcttagctac atttgctatt ttttcaaaat ctatgacttc
     1021 aagagttgga tttgcaagtg tttctgcaaa cacaacctta gtattttcct ttgcaagttc
     1081 tactatctca tcttcacttg aatctaagtc aaacgatatt aaatctatac caaatttttt
     1141 aagactaggt cctagtaaat tgaatgttcc tccatatact tttgaagaac aaagtatact
     1201 atctcctgat ttacatatat ttaaaactgc caacatattt gcagattgcc ctgatgatac
     1261 agctacagaa gatactccac cctctagtaa acttattttt tcttcaaaag cttgaatagt
     1321 aggattgctt attcttgaat atatatgtcc atcaactttt aaatcaaata attcagcaag
     1381 ttggtctata ctgctatatt taaaagttgt actttgatat aaaggtagta ctcttggttc
     1441 tccattacct ggtttataat ttccttgcac acatattgtt tctttattat acattcttgt
     1501 atccccctta gattttagta aaaaaaatta aactatttac cagatgagag ttaaaataaa
     1561 taaagcctga gtatataact caggctctgt ataagcttaa ttatactcat ctctcagctt
     1621 tcgccgcagg atttagcacc aatgtatata aatatactgg ttgccgggtt tcatagggcc
     1681 agtccctcca cctctctgga taagatttat ttaatttttc tatgttttat atcttatata
     1741 tttttcctaa ttttgtcaac tattttttta atttttttct tttaataatt ttcccaacct
     1801 ctacttgaat tacagacatt atggctaaaa caatcgttat aagccactct aagttattta
     1861 aaactgttaa gttaaaagca tttctaaaaa caggtacaaa cagtattact gctattaaaa
     1921 atgcagaagt taaaaatgag attaaaagaa atggattttt accattattt ctattaaaca
     1981 ttgaatctgt atttgaacgc tgactaaatg ctcgtaacat ctgtgaaaat gctaatacac
     2041 aaaatgccat tgtttgacct acagaatgat tttctatatt aagaccaatt ttatatgcta
     2101 aaatagtcat tattgccaca aatatacctt gggtaacaac tctttgtata agattttttt
     2161 caaacaaagt ggttgatttc actggctggt gcttcattat atttctgctt ggtgggtcaa
     2221 ctccaagagc aagggctgga agcgtagcag ttgcaaggtt tacccataga atatgaacag
     2281 caagaagcgg agtatcccaa tttaataata ttgctacaaa caacgtaagt atctctgcta
     2341 tattaccaac aagcaaaaat tgtataactt tttgtatgtt tctatataca cgtctacctt
     2401 cttttattgc ataagcaatt gttgtaaaac tatcgtccat taaaatcatg tcagaagaat
     2461 cttttgcaac atctgtacca cttataccca tggctatacc aatatcagca gactttaagg
     2521 caggagagtc atttacacca tccccagtca ttgctggtac ttctccaatt ctttttagag
     2581 attgtataat tctaagttta tcaaatgggg atacacgagc aaatactgta gtatttttaa
     2641 cagcttcatc tagttcatca tcactcaaat tatcaagttc atcaccagat atcacagtat
     2701 tttcttcact ccatattcct aactcatgag cgattgttgt ggcagtaact ttatggtcac
     2761 ctgttatcat tatggttctt atacctgctt ctttacaagt acttactgca tcaattacct
     2821 cagttcttgg tgggtcaatc ataccaacca tacctataaa tgttaaatca tattctatat
     2881 tttcactatc ttcttttggt atagatgaaa tctcacgctt agcaaaacca agtacacgca
     2941 atgcatcttt agacatgtta agacataatt cgtgtatatt ctttttatcc acttcagtaa
     3001 tgtttctttc accttttgaa gttaatattt tagtacatag gtcaagaatt tcgtctactg
     3061 ctcctttagt ataagcaata atcttttcat ctatattatg aacagttgtc atacattttc
     3121 tatctgaatc aaaaggctgt tcaaatacac gtggatattc ttcttcaaga gcttcttgat
     3181 ctattccaaa ttttttggcc ataaaaatta atgcgccctc tgttgggtca cctaatattt
     3241 catctgtttt atctggatta atacatgcat cattacaaag tgcaccagca taaacaatct
     3301 ctttatacac taatggatgt ttttcagatg cagtatctat tgatgtcact tttccagatt
     3361 caaaatctcc attgacagca atatgtgtaa cagtcatttt atttaaggtt aatgtaccag
     3421 ttttatcaca acatattact gtagctcctc ctaatgtttc aacagcaggt aattttctaa
     3481 ttaaagcatt tcttttagcc atacgctgta cgcctaaagc cattacaata gtagctgttg
     3541 caggtaatcc ctctggaata attgatatgg caagggaaat tgcaatcata aacattggca
     3601 agagaggtct ctgataaaat gcacctattg caaatataac aacgcataca ataataccta
     3661 tgacacttaa tgtctttcca acagtattaa gtttacgttt tataggagta tcaaaatcat
     3721 cttgtgaatc taaaagatgt gctatgttac ccacttctgt atccatacca gttgctacta
     3781 caacacctac acctctacca tatgtgacta ttgaagatgt atacgccata ttgctacggt
     3841 ctcctaatac acattcttta ggtaagattg tatctgcatc tttttctgat ggaacagatt
     3901 ctcctgttaa agaagcctct tgaattttta aacttgatgt ttctattaaa cgaaggtctg
     3961 ctggaaccat agctccatct tctaaaaaaa caatatctcc tactacaaga ttacttgctg
     4021 gaataacatt ttcttcacca tcacgcataa cacgagctgt aggtgaattc atatttttta
     4081 atgcttctaa tgatgattct gcttttttct cttgaacaat tccaataact gcatttactg
     4141 ttacaattat tagtattaca aaagcttctg taaattcgcc aaatatcaaa gataatattg
     4201 ctgctcctat tagtatcata accatagcat ctgtaatttg actccaaagc atttgtaaag
     4261 ttgttctagg tggttttttt ctaagttcat ttaagccatt ctctttaagc cgtttttccg
     4321 cctcggtatc ggtgagcccc tcctctgatg aatctagact ttctaaaatt tcatgcgttg
     4381 ctcttgaatg ccaaggcatc tgtttgtgat tttccatttt ggaagaccac tcctttcttt
     4441 ttttaattta agaataatta tattttaaaa tcaagttcaa ttcaatacac actaagtata
     4501 ttttacctaa atttaacaat gccatttaat atttaacaaa acttctactc ttttacaaat
     4561 atgtattcac tattgttaga taagttttct ttatacttat aaccttctaa atcaaacttt
     4621 ttcagttctt caatatctct tatcctattt tttacaatgt attttgtcat catacctcta
     4681 gctttctttg cttgtgtaga ttccactttc aatatgccat ttttatttac tttaaaagta
     4741 catgtaatat atgtatcact atctttaata aatttttcta tggatttgct gtattcattt
     4801 gatgaaagat taacgatagc tgttttttct ttgattagtt ctttatatat acgatttccc
     4861 caatattcgt ataaattctt aaactcacct acttgtaatt ttgtctgcat ttctaatcta
     4921 tattcatata tactatcata tggttttaca acaccataca atccacttat gattcgaata
     4981 cagctatttg caaactcttc atctttcctt gtaaaatttt ctgcttctat atttttatat
     5041 tgtattccat cataagttaa gattgcaggg tttcctaatc tatcaaattt aatgctttca
     5101 aatctatttt tatttaatac agctaatttt tcattaattt tcattttatt tttaaaatct
     5161 tctataccaa atgtctttat attttccact atttcttttg tgttatctat aaagcaaggc
     5221 aaactcaaat ctctatcaaa tacttctata ttcttcatat ttttagctgg tgataacatt
     5281 ataatcataa attcttatta ctcctgtttt tccaaatatt caagaatctc ttgtgcattt
     5341 gtgtctggtt ttgctttttc aaagactttt tcaataattc cattttcatc tataatatat
     5401 gtcgcacgta caactcccaa tgcagttttt ccatacattt ttttctcttt ccatacatca
     5461 aaagctttta tagcaactaa gtcagggtca gaaagtaaaa taaaaggaag ttcatgcttt
     5521 tctgcaaatt tttgatgtga ttttatacta tctttactta tcccaataac ttgtatatct
     5581 tcctttttaa atccatcata tgcatttcta aaagcacaag cttgacgtgt acatcctggt
     5641 gtattatctt taggataaaa atatactact acttttttgc ctttaaaatc tgacatactt
     5701 accttatttc catctttatc ttctagtgta aattctggtg cttttgttcc tatactcaac
     5761 ataaatatct ctccttttgt ttttattaat aattttatac tatgcttata ttattaccct
     5821 ataaatctat atataaataa aatattttat tttttatgta taaagtttca taaatatata
     5881 attttcatgt atttatatgt taaaatagac tgaaaatagt tttattaaga tatctatgct
     5941 taaaacaatg ttaaatgata gattaattaa aataaggata tctaaaaata aaatttttag
     6001 ttcatgagat gctctttttt gtatgaaatc aaatatattt tcatcatttc aacaataaaa
     6061 agagacttat ctctattttg agaaagcctc atttattatt acttttatac tatctatata
     6121 aatttaaatt cctcaacatc aaatagacct aaatctgtaa gtcttatttg tggaacaact
     6181 ggaagtgcca taaaagaaag tgtaataaaa gggtctacat actctggaac ttccattttt
     6241 cttgctatct ccaacatatt atcagtaatt tcttgtactt cttcagctgt aaaagtacta
     6301 attaatccag ctacttgaag acttaggtgt gcaagaactt ttccatttga tacaattaca
     6361 taaccacctt gtatttcctt taagtaattt atagctacta gaatatcatc atcattatcc
     6421 cctgcaacta taatattatg agaatcatgg gccacagtag tagcaattgc tccatttttt
     6481 atgccaaatc cttttagtgg agcaattgct acatttccag tcattctatg tctctcaaca
     6541 acacaaagtt ttgaatactc tttatttgga acaaaatatc catctgcaca tggtaaagat
     6601 tcatatacct tatttgtcaa aatttgataa ggtacaatct ccattacata atttttttca
     6661 cttactttta actgtatttt ttctttatta atatatttaa acttgactgt attaagcaat
     6721 tctttatctt taatctcata attgtaatta gaaaacatct cttcgtttac caaattacca
     6781 tctttatata cactatccac ttccattttg tctaaatcat tcaatacaac aatatctgcc
     6841 ttataacccg cacctattgc tccaattttc cttaacccat atgcttttgc agaattataa
     6901 gttgcaactt ttatagccct aacaggttcc atacctaaat caatcgcaca ttttatattc
     6961 catcttatgt gaccttgttt ttctatatca tctaagtgct tatcatctgt gcaaaacata
     7021 aattcttcaa ttggcaagtt atgctttagc ataccactta ctatagactt taaatttttt
     7081 gctgcgcttc cttctcttat taaaattttt aatcctgctc tcaatttttc atatacttca
     7141 tcaaaagtta tacattcatg gtcattttct attcctgcac atacatatgt ctgaagtgat
     7201 ttaccattta tattaggtgc atgtccatct acaactttat ttttaaaaag ctctagttta
     7261 tccaagattt cattttcaga atttataaca tcattaaaac acattacctc tcctaaacct
     7321 aaaattctag gattatttac ataactctcc atgtcttttg cactaaattt accaactcca
     7381 ttagtttcaa aacttgttgc aggtacggat gaaggcacca taatatatac attaagtggt
     7441 atatcttttg tcgattctaa taaaaaatct attgcactaa ctccttttac attgaccaac
     7501 tcatgtgggt cggctatgat agttgtcgta cctgatttta ataatttttt cgataattca
     7561 attggcatta ccattgtaga ttcaatatgc atatgtgcat caataaagcc aggtgatata
     7621 tatttgttac tacaatcaat ttctacattg cccttgtaat cgccaattcc aacaatagtg
     7681 tctcctacta ttgctacatc tcctgttatt aactcttctg taaatacatt tatgattgtc
     7741 ccatttttta gtacaatgtc agctttttct cttccacttg aaacatcaca ctgtcttttt
     7801 aaattaccca tttttacaac tcctcctaag ttttttatat caatatactg actgtattac
     7861 gtattatagt ttaatttatt aatatatatt atatatgagt tttgatagaa agtatcactt
     7921 gaaatttgtt ccataattcc tttaattaaa tagagagaac atgaggaata atattatctc
     7981 ccatgttctc attaatattt aagcaataat ttaattcata ttgaattttt tatttttata
     8041 tttaatcttc actattctta taaataaatt tatgtgcttt attatttttt taatacttct
     8101 ttattcataa ttatatttaa cctaaaaatg cataaactat catacctaca agtattgctg
     8161 gtatagtagt aataatcata gcccataaag aagatttttt atccattgct aaaagtggga
     8221 aaagtgcatc tccatcttga gaaatagagt tggctatcac tgcaccaagt ggtatagttc
     8281 ccttcaaata aaatgacata agcacaattt gtatcccaca acctggaata agtccaagac
     8341 ctgcacctat aaatacactt attacacctg ttgataatac taatttaact aagtattcat
     8401 ctccacctaa aattaatatt atcaaatcgt aaataagata tgcaataaaa atccatgtaa
     8461 ttacaaatga tatttctcca acagaatgga taagcatttc tcgcaaagaa atctttttat
     8521 tttcttcctc tttttgatta gaatttttaa ataccttttt aaaacaccac atataaatta
     8581 ttgaaaataa tattccaatc actgctatta cttcttccaa tgaatgtaac ttctcaatta
     8641 taggaagatt aagtccagag tgagcaagtg acataaatat aaaaccgatt actataattc
     8701 ctatataaat tttatatccc tttccatgtg ttattataaa tgcaagtcta tttgtatttc
     8761 catgagattt agctagagtg tctaaaatta tttcatcatg attatgttgt tctttatttt
     8821 ctttagggtt atttttactt atatcatatt tatctgattt tctacggttt tttaacccta
     8881 atttttcttc tagtttaaag taatccacta attgtccagt tattattcct gtaattgtac
     8941 taactatagt gacaaaaaaa tacattttaa tatttgatga aataagtaca aatgctgcat
     9001 ctcccatact agctattaaa cttgcaatta ttgctccaaa gcttaatttt ccatttatat
     9061 acaatggcat tattgccaaa cttccgccac aacctggtat agctccaatt aaagcaccta
     9121 ttaagggttg aaattttctg ttttttgata tgatattagt aaaatttcca ctagttttat
     9181 aattaatata tccaaataac aaaataaaaa atccaatcat agaacctaca tgtaaaaacg
     9241 cctcttcaga agcggatatc aacatttcca ttaatttacc tcctagacta attttgcaac
     9301 aattatatta tattctattt tctattgata atcaatatca atatttcttt tgaatttcta
     9361 atattaaaaa gtgcgcatct tatactctta gtttttcatc ttaagataca cactttttga
     9421 tattaggtta tctatttacc acatgtaata tgaaatcaaa ggtggatatt atgataaatt
     9481 ataacaatgt ccacctattt ttattaccta tcaaattata aattttcaag gaatgttaca
     9541 acttcttcta ttatccaatt aggtgtagat gcccctgctg taatacccaa atttttatat
     9601 tttttaagcg tatttacatc caattcatct ttagtctcta ttgcaaaagt tggtactaag
     9661 tcttcacaaa cctttactaa cttttgagta tttgaactgt gtttacctcc tataactatc
     9721 atacaatcaa cttctttagc taactcttta gctgattctt gtcttgtttt tgtagctgaa
     9781 caaattgtat cattaaatac tatttcttct aatttatctg atagtttatt aactatactt
     9841 atatacaact ctggatttat tgtagtctgt gcaacaagac aatatctttt ggagttatca
     9901 aattccatat tttccatttg ttctaaggtc tttattatta tagctgaatt ttcacaccat
     9961 ccatttatac ctataacttc tggatgcttc atatctccaa taactattat ttcatagcct
    10021 ttattatgat attcacttac tactgtatgt attttcttta caaatggaca tgtagtatcc
    10081 acaattttta attttttatc tttggcttcc ttatatatat tttcaggaac tccatgagac
    10141 cttattatca tattttcatg atttggtatt gtatcaattt cattcacagt cttaagacct
    10201 ttttcttcat attttgctac tgcttgcttg ttatgaataa gaggacctaa agcatatatg
    10261 ccagaatcat ttttttcaac ttcatcccat gccattttca ttgctctttt tactccaaaa
    10321 caaaagccag cattttttgc tatttttatg ttcataactt taccccctta ttttgaatat
    10381 ttaaagtaat tttgttttat cacttctaaa atctcttgtg atatcctttc atggtcatca
    10441 ttactcaatt tttccttaaa atgttcttca aatgatattg gttctccaat atatacaatt
    10501 actttactaa aaagtttata tttagatatt attgatactg gaacaacttt agatttactt
    10561 tttattgtaa atacagaaag acctgcctta gccttaccaa aagaatctcc ttttattcta
    10621 gtaccctctg gaaatattcc taatacataa ccgtctctaa ctgaccttaa aatagttttt
    10681 attgtagata catctggttt ttctctattt ataggcataa catgcaattt ttttaatata
    10741 aatcccaatg gttttacttt aaacaattct ttctttgcaa ttgcagctat ttctctattt
    10801 tctatggcag cagccaagaa tatagggtca agattcgatt tatgatttga tgctattact
    10861 atatttcctc tatctggaat attctctgca cctataactt catatttaaa aaatatttta
    10921 gaaaaaccct taaaaatatt tattacaaat ctataaaaat tcacgtttat ctcccttcaa
    10981 cactacattt attttcatta tgatgcataa aaaactaaag atttatttta gatacaactt
    11041 cttctacaac ttcttctata gtttttcctg tagtatctat ttctagtgca tcattagctt
    11101 ttaccaatgg tgcaaattct ctatttgaat caatttcatc tcgtcttatt atatcctcaa
    11161 ttacttcttc taaagtagta ttatatcctt tattacaaag ttctttatag cgtctatttc
    11221 ctctttcttc tggagtagct actaaaaaga atttgtaatc tgcatctggg aaaacatatg
    11281 aacctatatc tctaccatct agtattactg aatttttcat tcctattttt cgttgaactt
    11341 caaccatcaa ctgtcttact tcttttattt ttgctacatt agaaacatta ttgcttactt
    11401 ctattgttct tatttcttca tttataactt ctttgtccaa atatatattg ttatctttaa
    11461 aatctatatc cgaattttca gctatttgaa taacttcttt ttcattattg acatcaatac
    11521 cactttttaa acacttataa gtaactgctc tgtacatagc acctgtatct atataattta
    11581 tatttagctt ttttgctact atcttggcta ttgtactttt tccagctcct gctggaccat
    11641 ctacagctat aactaaatta cccatatata cacctctatt tattcatatt taattattaa
    11701 aaaattatat ttatacactt atacccgcaa gatatccagt tgaaaatgct atttgtaaat
    11761 tatatccacc agtttctgca tctatatcaa ttacttcacc tgtaaaaaat aagttcttaa
    11821 ctaatttaga ttccatagta gaagaattaa tttctttaac tgaaatacca ccagaagtaa
    11881 ccataccagt gtttatacct gtagtcccat tacaagttag cttcatattt tttatatact
    11941 caataattct aatttcatct gccttagaca aatcacttgc ctttttatca actaaagata
    12001 atagagaaaa aatctctttt aagaaatttt gaggtagtat ttgcttaaga ttatttagaa
    12061 catttttatt tggaaatgct ctgattatac tagagatttc atcagtactt aaatttggta
    12121 agaaatctat atttaattct accttttcct tttcaataat cttgttaata taagaagaca
    12181 ttataagaac acaaggtcct gttatcccaa aatgggcaaa tagcatgtct ccagattttg
    12241 atattttcct tttcttaatc ttacaagata tttccacatt cttcatggat attccttgta
    12301 aatctttaat ccatttttcc tctattgtaa gtggaactaa agcaggatat agtttgttta
    12361 atgtatgtcc atgcttttta agaatcttat acattgaacc atctgaaccc gtctttgaat
    12421 aactcactcc tccagttgat ataataacct tgtctcctag tattttatct ccattatcta
    12481 aaataatccc ttttattaga tactttgact tgtccttatt agaatcatct tttagagcaa
    12541 tttcttcagt tataatatct ataactttct tattatacat tatctttaca ttgtgattga
    12601 gtaaatcttt atttaatact tctattactt ctatagattt atcattttta gtgtatactt
    12661 tgtggtcatt ttcttcttca atcttatatt ccaaatttct actttcaaaa tacgaaataa
    12721 ggtctttatt tgtaaatgta taaaaactac tatagagaaa ttttttatta ctgacaactt
    12781 tatcaaaaaa atcttctatc tctctattat tggtaaaatt gcatctaccg ccaccagttg
    12841 ccctaagttt tcttcctaat tctccatttc tctcaactaa tattacttca ttattgtttt
    12901 ttgaagctga caaagctgcc atcattccag aagctcctcc accaactact ataactttac
    12961 tcataaattt ctccttataa ttgattccta ctccagtgca gaattattta tctttacatg
    13021 attttttata tgacttgtat cattcatttt tactataaca ggcccataat ctttaaattc
    13081 tactatattt aaagcagtat tatcttgctt aattctatat atattttcca tacctgattc
    13141 taaaaatgac aacaacataa ccctcaatgt aattgaatgt gtaacaagaa ttatattttt
    13201 attatcatat ttttcattta gttcctttat aaaagcatct actctttcct ttataatctt
    13261 aagagtttcg ccttctggta tatttactaa gtgtggttca tttctccatg ttgcatatat
    13321 atctgaataa tctttttgaa tttcttttat taaaaggcct tcccaaactc cgaatcccat
    13381 ttctctaagt gcctcagttt tttgaacttc aatattgagc ttatctccta aaatttgagc
    13441 tgtttgcatt gctctcccaa ggtcgctact aaaaatataa tcaattgaat acttgccaat
    13501 atcttcagaa agttcttttg cctgttctat accttgaggc gtaaggtcag aattaccatg
    13561 accttgtgtt tttcccaata tattccaatc cgtttgtcca tgtcttacta tataaaaagt
    13621 atttcccata tgtcctccta atatatgcct attttttatt aaatataaat tatcacttct
    13681 caaaacttaa ttatatcata taatacttta ctttgtttct attttataat ctaatctaac
    13741 atattattt
//
LOCUS       DAGSHT010000087        13690 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGSHT010000087 DAGSHT010000000
VERSION     DAGSHT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.089"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            126..242
                     /locus_tag="MJB72_002191"
     CDS             126..242
                     /locus_tag="MJB72_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBY8263296.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            406..1473
                     /locus_tag="MJB72_002192"
     CDS             406..1473
                     /locus_tag="MJB72_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8263297.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1665..2195
                     /gene="rnmV"
                     /locus_tag="MJB72_002193"
     CDS             1665..2195
                     /gene="rnmV"
                     /locus_tag="MJB72_002193"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBY8263298.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2366..3235
                     /gene="rsmA"
                     /locus_tag="MJB72_002194"
     CDS             2366..3235
                     /gene="rsmA"
                     /locus_tag="MJB72_002194"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBY8263299.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3301..4563
                     /locus_tag="MJB72_002195"
     CDS             3301..4563
                     /locus_tag="MJB72_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263300.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4714..5829
                     /locus_tag="MJB72_002196"
     CDS             4714..5829
                     /locus_tag="MJB72_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY8263301.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6015..6911
                     /locus_tag="MJB72_002197"
     CDS             6015..6911
                     /locus_tag="MJB72_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY8263302.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            6981..7778
                     /locus_tag="MJB72_002198"
     CDS             6981..7778
                     /locus_tag="MJB72_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263303.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7833..9185)
                     /locus_tag="MJB72_002199"
     CDS             complement(7833..9185)
                     /locus_tag="MJB72_002199"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBY8263304.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9404..10282
                     /gene="purR"
                     /locus_tag="MJB72_002200"
     CDS             9404..10282
                     /gene="purR"
                     /locus_tag="MJB72_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBY8263305.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10438..10716
                     /gene="spoVG"
                     /locus_tag="MJB72_002201"
     CDS             10438..10716
                     /gene="spoVG"
                     /locus_tag="MJB72_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBY8263306.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            10929..12308
                     /gene="glmU"
                     /locus_tag="MJB72_002202"
     CDS             10929..12308
                     /gene="glmU"
                     /locus_tag="MJB72_002202"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBY8263307.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12403..13353
                     /locus_tag="MJB72_002203"
     CDS             12403..13353
                     /locus_tag="MJB72_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBY8263308.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT         5530 a         1459 c         2521 g         4180 t
ORIGIN      
        1 aaaaataaaa ggtaaattgt ttctggataa cttaaaattg gaagtaagaa tatttaacta
       61 gagaagaatt atttaagttt aggttagctc taggagtagc tgattttata ggaacaatag
      121 attttatgga tgatgatata aacatggtag caattagacc agaagatata agggtagaat
      181 ttaatagaga tttcaaagaa attatagacc tcaaactcag accgtatcga tataaaaact
      241 aaagttagat gatatagtgt tgtagatagg accatcagct gttatggcag tattagtgct
      301 tttaacaaat ataatagtaa aacttttgta tgaattgata ataaaaaaat taaaaaccaa
      361 gacataaaaa tggcaacaaa aagaagttga ataagggagg taaaaatgaa atttaagaag
      421 ttagtctctt tgggaatatt atcaacactt ttattgagtg taataactgg atgtagtaat
      481 gtttcatcag gttcaaactt aaagtctaat aataagacaa aaggagagtt aacagtttat
      541 acagcaattg aggaagatag tatagataca tacttagcta catttaaaga gaaatatcca
      601 gatattaaat tgaatgtact tagattatca gtaggaggta taacagcaag attattagca
      661 gaaaaagata atcctaaagc agatgtgtta tggggtgttg cagctacaag tttgataatt
      721 ttagatgaca aagaaatgct agagccatac gctccgaaag gctttgataa ggtaaattct
      781 gcatttaaag atgataagaa agttccttct tgggttggaa tagatgcttg gatgactggt
      841 ataacagtaa atactaaaga attaaaagct aaaaatatat cagctcctag ttcttatgaa
      901 gatttaataa agccagaata taaagatctt atatctatgc ctagtccagc ttcttcagga
      961 acaggatatt taacagtatc agaactcata caaattatgg gtgaagaaaa agcatggcag
     1021 tatatggata aattgcataa aaatattggt gtatacactc agtctggttc agctccagct
     1081 atattagcag cttcaggaga atatcccatt ggtatatctt ttggaaatag aggtataaag
     1141 ttaaaaggtg aaggttatcc agtagaagta gtatttccaa aagaaggttc tggatgggag
     1201 atagaatcaa atgcacttgt gaaaaaagat aatataaaag aagcatcaaa agtattttta
     1261 gattgggcta taagtaaaga tgcaatgaat gagtattcta agaattttgc agttactaca
     1321 attgacacgg gaaatccggt gccagaagga tttccaaaga atccattaga acagatgata
     1381 gataatgatt taaaatcagc agcaagaaat agagaggcta tactaaataa atggatagaa
     1441 aaatatgatg gaaagacaga aaaagagagc taagccttgc aataaaattt agtacaaaaa
     1501 gcgcctagaa tataagtttt atatctaggc tcttttgata tatttattta aaatgcatat
     1561 ttctatataa aaataaatag tttagtacct tgattaaatt aaataaaaac cttaaaataa
     1621 agagatgtaa agaaaaacta aaaacgaata aaaggtgaat ttatatgata aaagaaataa
     1681 tagtagtaga gggtagagat gacgttacag cagttaagag agcaatagat gcagaactta
     1741 taactacagg tggatttgga tttccaaaag gtgtaatgga gcgtataaaa gctgctaatg
     1801 aaagacgtgg tgttataata tttacagacc cagattttgc aggtgaaaaa ataagaaaaa
     1861 aaatagcatc agaagttcca ggatgtaaac atgcatttct accgagagag gaagctaaaa
     1921 aggatggaga tataggtatt gagaatgcta ctcctcaaag tataatagca gctctgaata
     1981 aagttagaac tgaaaataca gaaaaaagag atgaatttaa gcaagttgac ttaattagaa
     2041 atggtcttat aggtaatgaa gaagcatcac ttagaagaga tgcacttgga aaaatacttg
     2101 gaattggata tggaaatgca aaacaatttt taaatagatt gaataattat ggagttgaaa
     2161 gagaagaatt tatagatgca ttaaaacaaa tctaaacgta atgtttaatg tatgaaaaat
     2221 atgtatataa aataaattgt ggaattacgg ttaatttaag atgaattatt tataatatac
     2281 accttatttt aaatcaatgt aaatacatat aaataagtat aattaataaa attaaatatc
     2341 aaaataagtt aaaaaggagt aaataatgga tagactatct tcacataatg caacaaagga
     2401 agtagtacaa aaatataatt ttaaattttc aaagtcttta ggtcaaaatt tcttgataga
     2461 tagtaatata attgataaaa tcttatctgg agctagaatc acaagaggag ataatatcat
     2521 agaagtagga cctggtatag gaactcttac tcgtgagatg ggtaaaatag cagaaaaagt
     2581 agtggctata gaaatcgata gaaatttaat tcctatattg aaagatacct tgtcagattt
     2641 agataataca gaggttgtaa atcaagatat actaaaagta gacatacagg aattagtaaa
     2701 agataaatta aatggtgggc cagttaagct tgtagcaaat cttccatatt atataactac
     2761 tccaatagtt atgaaatttt tagaggaaga catacctgta acagacatag tagtcatggt
     2821 tcaaaaagaa gttgcagata gaatgaatgc tattccgggt actaaagatt atggggcatt
     2881 atctatagct gttcaatact attgtgatac agagattgtg gcaaaagctc caagacatat
     2941 gtttatacca caaccaaatg tagattccac agttataggt ctacatgtaa gagataaaag
     3001 aaaatatgat gttcataatg aagacatatt ttttaaaaca gtaaaagcat cctttggaca
     3061 aagaagaaaa actcttctta attcgttggg aggtcttggt tttcttaata aagatgaaat
     3121 aagagaagta ttaaaagaag ctaatataga cgaaaaaaga aggggtgaaa ctcttagtat
     3181 agaagagttt tcagttcttt caaatataat aaatacaaaa gtatcttcta aatagaaggt
     3241 acttttttta attttgttaa catatattgt ttagagaaac tgtgttaagg ggggattgca
     3301 gtgggttcta aaagaaagtt taaaagagac tatataatac tagaagctaa agacataaac
     3361 tttagataca aagaacgagt actgccaaaa gcttttgcaa aagttgaagt aaatgatgaa
     3421 aaagcaatta ttgcattata tgtagaaaat ctaaaatatg ttaaagatgg gtataaagca
     3481 gtagcaataa aagatgacca tggattaata gacttaggaa atataatttt aaatgaacaa
     3541 ggaagaggtg aatttgtttt agatttagaa gattatgatg atgagataaa aggtattgca
     3601 ttattgtatg aaagaaatat acctttaatt ggatttaaag gaaataagct tgaaaattat
     3661 gaggacatat tatttcaaga ggtagaggaa gaagaatatg aagaatatga agagttggaa
     3721 gaagaagacg aagagataga ggagaatgag gaaagtgata gagacgaaga tagtgaagaa
     3781 gaatatatta tagaagaata tgatgaagat gagtatgaag actattggga agatgatgaa
     3841 gatattgaag aaggagagtt aaaagcaaga acggaagtag aagattctga tgatgaggaa
     3901 atagaagagt tcaataatta taaggaaata gaaaattata atagggttaa agtacaggaa
     3961 gaatatatag acatagaata tgatgaagaa gaagaggaag aagaagtttt tgaagtaaat
     4021 agagaagaag ataggtttgt ttcctcaaaa cctactaaat caaagaaagt tcaaaaagat
     4081 cagaataata ttgaaaaaat aaaaagagct aatggagtaa gtcaaggaga gagtggaaat
     4141 aaacaaacca gtacattact tatgccaaga caaataaaaa aaggtttgaa attatttaaa
     4201 gaagtaaaac cttttgtaac agattatata gataagacaa gatggtggaa aatagagata
     4261 aatcctacaa cactgtgtgg atatacaatg ccttatttag gatatttaaa cgtattgaat
     4321 tacactatgt atagtgatgc agttttacat tcatataaat acagacacta tttatttgga
     4381 gttcagtatg atgaatataa taagagaaaa cattatattt atgctatccc aggaaataga
     4441 aatgaacagc cagacaatgg aagcacagga tttttaagat atcaagcttg tgataataga
     4501 acaaatagtt taggatattg gctatgtttt atagattcta gacaaagaaa tatagttagg
     4561 taaaaataat aaaaatgaaa aaatttagac tcaattctta ttttactgaa aaaattaatg
     4621 aaatgtagta caaaacttta aaaactattg ataatattta aatattacac tacaatatat
     4681 cttgaaatac ctattatatt atggaggaaa attatgataa acgagcaaag aattttaaat
     4741 gagtttcttg aattagtaca gatagatagt ttatctttaa aagaggggaa tgttgctaaa
     4801 gtattagtta aaaaattaga agaaataggt tgttcagtag taatagacaa tgctggtgaa
     4861 aaagcaaatg gagaaactgg taatataata gctacattaa aaggaaataa agaaggaaag
     4921 aaaatattat ttagttcaca tatggataca gtaactccag gtataggggt aaaacctata
     4981 gtggatgaag caaatggaat aataaaaagt gatggtacta caatattagg ttctgatgat
     5041 aaagcaggta tagcagctat attagaaggc ttaagatata taaatgaaaa taacatagaa
     5101 catactgata tacaagttgt attctctata tgtgaggaat gtggattagt tggagctaaa
     5161 aatttagact atggaaaaat agattctgaa tatgcattta tattagatag tggtggttct
     5221 ccaggtgaaa taatagtaaa agctcctgca caagatgtaa taaatgttaa gatattagga
     5281 aagactgctc atgcaggtct tgaacctgaa gctggaataa gtgctataat ggtagctgcc
     5341 agagcaatag aaaatatgaa tttacttaga atagacgaag aaacaactgc taacatagga
     5401 attataaacg gtggaacagc tacaaatata gtaacaggag aagttaatat agttgcagaa
     5461 gccagaagtc taaaagaaaa taaattagat gttcaaacta aacacatggt agagactttt
     5521 gaaaaagctg cgaaagattt tggagctcaa attgagatag atgttaatag agcatacaca
     5581 ccaatagatg ttagtgagga ttctgaaata ataaaacttg caaagaaggc tttttcaaac
     5641 ttaggtatag aaggtcatac tgaatctaca ggaggaggta gtgatacaaa tatattaagt
     5701 aagaatggta tcgaagctat aactttagga attggtatga aaaatgctca tacactaagt
     5761 gaacatatag ctataaaaga cttatatgat tctgctaaaa tggtagtgga gataataaag
     5821 gaagcataaa tttaaataat agtgtataat atatatatcg cctagcttaa gctaggtgat
     5881 ttttcatttt atagaagatt gataatctct attgaaagca aagagtatac atttgcatat
     5941 aagagagtgt ttaatttatg ttcaaatgta ggttcttaat aatatttaga ttgtttcata
     6001 agggaggaat aaatatggaa acaaggtatg aagaagcaaa taaaataaca atacaatcaa
     6061 tactttggaa tatagtatta acaataatta aggttatagc tggtgttatt ggtaattcta
     6121 gcgctatgat tgcggatgga ttacattcag catcagatat tataagttct ataggagtac
     6181 taataggcaa ttatgtatct tctagacctg gagatagaga gcataattat ggtcatgaga
     6241 aagcagagac attagtatca tttgtgttat caatattgct tattttcgtt tctataacaa
     6301 taggtataga ggcaattaaa tctttattta acttagatgc attaagtgtt ccatcaatat
     6361 taccattagt agtatcagtt atttcaatcc tgataaaaga gtatcaatat agaataacaa
     6421 taaaagttgc taaaaagata aattcaccag ccctaaaagc tgatgcttgg catcacaggt
     6481 cagatgcttt atcttcagta gctgctttta taggtatagg tggttctatt ttagggttta
     6541 aaccattaga cccaattgcg tcagttgtag tggctatatt tgtagctaaa gtaggaatta
     6601 gtatacttat aagttctgtt aatgaattga tggatgtttc agttgatgaa gaagagataa
     6661 aagaattaaa atttattgta gcagatacag aaggtgttaa aaatcttggt gatataaaga
     6721 ctagaaaaca tggagctatg gcatatgtag atttgactat atgtgtggat gaaaatttga
     6781 cagttaagca aggtcatgat attgctacca agcttgaaaa acatataata aaacatatgg
     6841 aatttgtaaa aggtataaca gttcacgttg aaccatgtac taattgccaa ggaaataagt
     6901 gtaataatta attaaaactt aaaaaaaagt gtaaaaagtt atataattgt tttatgatta
     6961 taaactctag gggtggttaa atgagcaaga aaagaaaact aaagaaaaaa gctgcaatat
     7021 taataggagt agttgttttc tttataatat actttatatc ctttagaata ggaataaata
     7081 tggaaaagga taaaagtgaa gtgccaaaga aggtagaaaa agaagaagaa gataataggt
     7141 ctttaaggac tcaaataaaa gagaagaaga aggtttatat atcagataaa aatatagaaa
     7201 acataaaaat tgaagaagat atgtgggatg aaataaagtt atttttctca gattttaaga
     7261 aagtaagaaa tactacttct tcttacgaag cagtttatga aggttattct gatgatggag
     7321 ttaaattttc tactgacctt aactttttta gagtatatac tgtaaataaa gaagaatatt
     7381 ataaagtacc agttagttct aaggatgctt ttaagaaact tttagatgag agtatatata
     7441 cttcgtttga ccttttaagt cattataaag attggaaaga ggttactata actcatggaa
     7501 aagaaattaa aaaagttcat agatggaaat ttgatgattt agcacataag atgatttcaa
     7561 agagggttgt aggaaaagtt caacctgaga aaagtaagga aagaagtgac tataatttta
     7621 ctataaatat aaaaggtgat aattttgatg taaaagttga tacaatggga aaagattatg
     7681 taaaagttac ttctaagaaa aatgtatctt actatgaggt tcatacaagt ttatttgatt
     7741 accttaaaaa tgagatattt gaaattggaa agaaataaga ctttaagaag gctatcaaat
     7801 tagatatttg atagcctaaa ttattttata tattattttt caaccaacaa gtctgcaacc
     7861 ttataaattg gaccagcacc agcagttatt actaaatcat tatccttaac attttcacgt
     7921 aagtatttta ctatatcttc aaattctttt atatagatgg catctacatt gttttgatat
     7981 aatttctcaa ccaagtcctt agaatgtata tctcctgggt ctttttccct agcagcatat
     8041 atatctgtta tgataacttt atcagcagca tagaatgcct ctgaaaattc acctaaaaga
     8101 gattttgttc tagtataagt atgtggctgg aagatacacc ataaagttga tttttttaat
     8161 tttttggcag cagctaaagt tgcttttaac tcagttggat gatgtgcata atcatctact
     8221 actaaagcat tcttatagta ccctttagtt tcaaatcttc ttccgacacc tttataaatt
     8281 tttatatttt ttcttatagt ctctaagtca ataccagaaa caagagctgc cattatggca
     8341 gaagaagcat tgtatatgtt atgaagacca tatacagaaa gttcaaattc tcctaaatct
     8401 cttccttcat attctattct aaatattcca tgaccactat tgtcaaagtg aatatctttt
     8461 attacagcat cattgtcaga atctctgccg tattttataa tagtagcttt aacatcatac
     8521 aatatgtcat caatattttc atcatcgcca tttattatga aatagccttc tgggggtaat
     8581 aattttccaa atttgttaaa agaagccttg atttcatcta ttccagagaa ataatcaaga
     8641 tggtcttctt caacatttaa aactatagat atctttgggt taaaattaag gaaactatct
     8701 acatattcac atgcttcagt tataaagtgg ttggaattac ctatcttaac attaccacct
     8761 atcatactta agttaccacc aactagtata gttgggtcta agtctgcata ttcaaagatt
     8821 gtagataaca tagatgttgt agaagttttt ccatgtgttc cagaaactgc tatagaattt
     8881 ttatattccc tcatgatttg gcctaaaaaa gttgctctgt tcattaccaa tttatttttt
     8941 tctttagcag ccacaagttc ttcattgtct ggatgaactg ctgctgtata aacaaccata
     9001 tttacatcat ctgatatatt atcttttttc tgaccaatat aaatagttgc cccttggtcc
     9061 ctaagtttgt ctaataggta tgattcgttt gaatccgaac ctgaaacttg ataaccttta
     9121 ttgatacata tttcagctaa tgcactcatg ctgataccgc caattcctat aaagtgtata
     9181 ttcataataa aaccaccttt ctttttaatt aagtcctaca atatgaacta tttctgtggt
     9241 ataataatat aatgttcaat attttataat taatatgcat ataatattat atgtatttta
     9301 tttttgaata taataaaaca taagtaatat tataccacaa taagaattgt tatgcattag
     9361 ttagtttaac gttatataaa ggtacttgta ggaggaaata ataatgaaat ttaaaagaac
     9421 agagagaatt ggagcaatag ttaaaatact gtctgataat ccaaataaaa tatatacttt
     9481 aagttatttt acaaatcaat ttaatgcagc aaaatcaaca ataagtgaag atttattagt
     9541 tgtaaaaaat gtatttgaga agctacattt aggtaaagtt ataactattt caggagcagc
     9601 aggaggagta aaatatatac ctaaaacttc tatagcagaa aatgcagagt tcttaatgga
     9661 attatgcgag agaatatgtg acaagtctag aatactatct ggtggttttt tatacttgat
     9721 tgacctaatt tacgacccta caatagctgc taagatagga aagatattcg catcaaatat
     9781 tgagtatgta gatgcagatt atgttgtgac aatggaaaca aaaggtatac ctatggcact
     9841 tatgactgca aaagctatga atttacctct agttataata agaaaagata taaaggtatc
     9901 agaaggtcct acacttagca tgacatatgt gagtggaaac agttctaaag ttgaaagtat
     9961 gagtttgcca agaaaagctc ttaaaccaga tagtaaagtt ataattatag atgatttcat
    10021 gaggggtgga ggtactataa aaggaatggt agaccttatg aatgaatttg gtgcagaggt
    10081 tataggtact ggagtattca tatctacaac gaatccttca gaaaaaatgg taaaagatta
    10141 tatttcattg attcaattgg atgttaatgg agataaaata gtagtagaac caaacttaaa
    10201 gacttttaaa gatgaatata gaaatgaaga tttagacgaa gaagaagatt cagaatttga
    10261 atttgagata gatgaagatt aataaaaaaa atatttagaa aaatatgtaa aaataaaaag
    10321 aggatatccc tagttgttca tagaattatt taaaaaacag tttatattaa tttattccag
    10381 cgataataat tttaatataa attaatttct gtcaaaatca aagggggata ttttggtatg
    10441 aagataactg acgtaagagt aagaaaatta acagaagaag gtaagatgaa atgtatagtt
    10501 tcaataactt tcgataattt gtttgtagtg catgatataa aagttataga aggccataat
    10561 gggctattta tagcaatgcc tagcagaaaa gtaggcgaag gaaatttcag agatatagct
    10621 catccaataa atgcagaaat gagacaagtt ttagaagatg cagttttaca agcgtatcat
    10681 gaagcattag ttcaatggga agtagctgct gaataataat aaatatataa aatggagaga
    10741 gcccgaacat ttaggctctt tttaaatgta gaatttctat taaaatatgt actttttagt
    10801 aaaaaagtgc tatattatta gtgataatgt atatgttgta tacaaattgt tagataaatg
    10861 tattataatg tatattgatt aagagaataa tacgaataaa attaaattag attgaatgga
    10921 gtgtatcaat gaactttaaa gctataatcc ttgctgctgg caaaggaaca agaatgaagt
    10981 ctaaataccc aaaagttata cataaagtat gtggaaaaga aatggttaac catattatag
    11041 atgtttcaaa aaaatcaggg gtaaaagata ctgtcgtgat tttaggacat gaagctgatg
    11101 tagttaaaga aaagcttgca gaagaaatta taatagccat gcaaacagaa caacttggga
    11161 caggacatgc tgtaaagatg gctaaagaat atattaatga tgaagataca atagttgtac
    11221 tttgtggaga tactccactt ataaaagaag agacactaaa gagactattt gaatatcata
    11281 tagaaaataa atatcatgct acagttctta caacaagagt tggaaatcca acaggttatg
    11341 gtagaataat aagagataag aagggtgacc ttcttaaaat agtggaacaa aaagatgcaa
    11401 attcagaaga aaagatgata agtgagataa actctggaat ttattgtttt aatggaaaga
    11461 gtcttagaga agcactagat ttgctaaata ataacaattc acaaggtgaa tattatttaa
    11521 ctgatactgc taaaataatg agagataaag gattaaaagt tggtgcattt gcaggttcaa
    11581 ctattgaaga acttatgggc gttaattcaa gagtagaatt atcaaaggca gaagaaatta
    11641 tgagacgaag aataaatgaa tctcatatgg taaatggagt aactataata gacactaact
    11701 ctacctatat agaatctgat gttatgatag gcaatgatac tataatatat ccaggtgtta
    11761 tgttacaagg aaaaacaagg ataggttcag attgtataat tggaatgaat tcatctataa
    11821 caaattcaga aataggtgat ggtacagaaa taaaaaattc aactataatt gatagtaagg
    11881 ttggagaaaa ttctactgtt gggccatatg catacttaag accaaagagt gacttaggaa
    11941 ataatgtaaa aataggcgat tttgtagaag taaaaaatgc tattatagaa gatggctcaa
    12001 aagcatctca cctttcttat ataggagatg cacatgtcgg taaaaatgta aatattggat
    12061 gtggagtagt atttgtaaac tatgatggta aaaataaatt taaatcaata gtaaaagaca
    12121 atgcgtttat aggttcaaat tctaatttag ttgcaccagt agttgtagaa gaaaaaggat
    12181 atatagcaac aggttcaaca ataacacatg atgtcccaga tggtgctttg gcaatagcaa
    12241 gagaaagaca agttatcaaa gaaggatggg tagaaaaaaa gaatcaaaaa gacgaccaaa
    12301 gtaagtaatt atgtagttta ttattttggc taaaaaattt agctcgaaat tattataaat
    12361 tatattcatt agtattttaa aattttcagg agggttacat aaatgaatac tagcggaagc
    12421 gagattaaaa ttattgcagg taattcatcg aaagagttag cacaaaaaat agctgactat
    12481 ataggtgtat cagtactaga ttgtgaagta ggtacattta gtgatggtga aatatgtgta
    12541 aacatgaatg agacagtaag aggttgtgat gtgtttgtag tgcaatctac taacagccca
    12601 gtaaatgata acttaatgga gttattaatc ttaattgatg cgttaaagag agcatcagca
    12661 ggtagaataa cagcagttat tccttactat ggatatgcaa gacaagacag aaaggctaag
    12721 gcaagagatc caatcactgc taaattagtt gcaaatttaa taactgctgc tggagcagat
    12781 agagtattaa caatggattt acatgcagct caaatacaag gatattttga tataccatta
    12841 gaccacttac taggtggaac aatattagca aattatttta atgaaaagaa aatagaagat
    12901 ttagtagtag tatctcctga cttaggaagt gttactagat caagaaaatt tgctaatact
    12961 ttaaatggag aagttccaat agctataata gataaaagaa gaccgaaagc taatgtatgt
    13021 gaagttatga acttaatagg tgatgttaaa ggtaaaaatg ttatactatt agacgatatg
    13081 atagatacag caggaacaat agttaatgct gcaaatgcac ttaaagagtt tggagcaaaa
    13141 gatgtttatg cttgttgtac tcatggtgtg ttatcaggtc cagctattga aagaattgct
    13201 aattcagaaa taagtgagtt aatagttctt gatactatac aacttcctga agagaaaaga
    13261 atagataaga taaagataaa aacagtagct cctttatttg gagatgctat aagaatgata
    13321 ttctctaatg aatcagtaag taaattattc taatttattg tatttatgag agttgtttaa
    13381 aaggatataa accttttgag caactctttt ttgattttat acttatggaa tgttattttt
    13441 ttcttgttag aaaggtatat attattgtaa aaagagatta tagtttaaaa ttatgtatat
    13501 ttaagtaaaa taaacctaat aaagggtata ttattcaaaa tattatgaat ataaaaaata
    13561 ttacaaaaaa gtattgaaaa aattcagatt atgtagtatt atgtaaatat aaataaagaa
    13621 agaggtgttt ccaatgggaa ggttagaaga tgaaccccta ataattaaat gattcagtca
    13681 caatctaaag
//
LOCUS       DAGSHT010000088        13657 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGSHT010000088 DAGSHT010000000
VERSION     DAGSHT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.090"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            353..2452
                     /gene="nrdE"
                     /locus_tag="MJB72_002204"
     CDS             353..2452
                     /gene="nrdE"
                     /locus_tag="MJB72_002204"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBY8263309.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2465..3436
                     /gene="nrdF"
                     /locus_tag="MJB72_002205"
     CDS             2465..3436
                     /gene="nrdF"
                     /locus_tag="MJB72_002205"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBY8263310.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3512..4138
                     /locus_tag="MJB72_002206"
     CDS             3512..4138
                     /locus_tag="MJB72_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY8263311.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4533..5162
                     /locus_tag="MJB72_002207"
     CDS             4533..5162
                     /locus_tag="MJB72_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263312.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            5163..5945
                     /locus_tag="MJB72_002208"
     CDS             5163..5945
                     /locus_tag="MJB72_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263313.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5938..6816
                     /locus_tag="MJB72_002209"
     CDS             5938..6816
                     /locus_tag="MJB72_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263314.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6849..7808
                     /locus_tag="MJB72_002210"
     CDS             6849..7808
                     /locus_tag="MJB72_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8263315.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            8121..8813
                     /locus_tag="MJB72_002211"
     CDS             8121..8813
                     /locus_tag="MJB72_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263316.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8823..9827
                     /locus_tag="MJB72_002212"
     CDS             8823..9827
                     /locus_tag="MJB72_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263317.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            10075..10926
                     /locus_tag="MJB72_002213"
     CDS             10075..10926
                     /locus_tag="MJB72_002213"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBY8263318.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            11030..11776
                     /locus_tag="MJB72_002214"
     CDS             11030..11776
                     /locus_tag="MJB72_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263319.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11776..>13657
                     /locus_tag="MJB72_002215"
     CDS             11776..>13657
                     /locus_tag="MJB72_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263320.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKSEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYIVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNY"
BASE COUNT         5366 a         1338 c         2439 g         4514 t
ORIGIN      
        1 caatatatca gaatatattt ttaagctatt tagagataat ttaaattttt aaacaggaca
       61 taataagctt gtattgtttt atatttgagt tgataaaaat ttctttaaat aaaaataatg
      121 tcaattataa agtaataaaa gtttagatat aaaaaaattt gtgtatatac tatatgtagt
      181 atataataga taaagcactg actatatgta gtgcttttgt ttttgaaaaa tacttattgc
      241 atttctataa ttatagatta attttgggat ttcaactgat aagtaccaaa agaaataata
      301 agcaaattaa gtgaaattat ggcacaagat tatgttaaaa ggacggtaaa taatgaattg
      361 gatagagctt aacaatcaag tgataattaa aaatgaacaa ggcaagtacc aattggaaaa
      421 ggataaggaa gccttaagta gttacataga agaatttata cagccaaagt tgagaaaatt
      481 taataaccta gaagaaagat tgaagtattt aatagatgaa ggttattatt caaaagaagt
      541 aataaatcag tatagcatag atgttattaa aaatatatat gatatgattg ataaacataa
      601 gtttgaattt caatcatata tgagtgcaaa taagttttat caaaattatg cattaaagag
      661 caatgatgga aaggaaatat tagaaacata taatgataaa gtgttaattg tagctttgac
      721 actaggaaat ggcgataaag atttagcttt aaacttggca gataaaatag taaaacaaga
      781 atttcaacca gctacaccta cttttttaaa tgctggaaga aaaagggctg gagaaatggt
      841 atcatgtttt ttactatctg tagaagatag tacagaaggt atatcgtatg ctatatcttc
      901 atcaaatcac ttatcaaaga ttggtggagg agttgcacta aatttatcta aacttagagc
      961 atctggcgaa tctatcaaag atatagaagg tgcagctgga ggtgttgttg gagtagctaa
     1021 aatgttagaa caatcattta gttactttaa tcaaatggga gctagacaag gttctggtgc
     1081 ggtgtacctt actgtattcc atcctgattt tgaattgttg atggatacta aaaagataaa
     1141 tgcagatgaa aaaataagac ttgctacatt atcattagga gctataatac cagataaatt
     1201 tatggagttg gcagaaaaaa atgaagtagc atatgcattt tatcctcata ctgtgtataa
     1261 gaaatatgga gtttctcttg atgagataga aatggatgag tggtatgata aattggttaa
     1321 tgatagtgat ataagaaaaa aagaaataaa tcctaggcag atgttgacaa aaatagcaca
     1381 gatgcaacaa gaaagtggat atccatatgt agtgtacata gatacagcta atagagaaca
     1441 tacattaaaa gatgttggaa ttattaaaat gtctaactta tgttgtgaga tatttcaata
     1501 tcaaacacct tcagaaatag aggggtatgg aggaaaaaat gaatggggac aagatataag
     1561 ttgtaaccta ggttctttaa acatagctaa tgtaatggat aataaaacta tagaaagtac
     1621 agttgaaaca gcaataagag cactgtcatt tgtagcagat agcacagata taaaaccagt
     1681 gccaacggta agcaatagta acagcaagtc tcattctata ggattaggag ctatgaattt
     1741 acatggatac ttagtaagag aaaatatttt atatacttca gatgatgcta tagatttttc
     1801 taatgtattt tttgcaatgg ttagatacta ttcaattaaa gcttctatga aaatagctat
     1861 agaaagaaat caaacttttg aaggatttga taaatcagaa tatgttaagg gaagaaatag
     1921 caaggtttta tcaaaatact atgaacaatc ttatctacct aagtcagaaa aggtaagagc
     1981 attatttgaa gggatttata taccaacaaa agaagactgg acaaaattat tggatgaggt
     2041 aaaagaaaaa gggatatata atgcatattt aatggcagta gcaccaactc aaagcattag
     2101 ttatgttcaa aatgctacat caagtataat gccaattact gagccagtag aagtaagaac
     2161 atatggagac tctactacaa tatatccaat gccattttta acaaatgata atatgttgta
     2221 ttatcaatca gcatacagga tggatatgag aaaggtcata gatttagtgg caacagttca
     2281 aaaccatgtt gaccaaggta tatcaacaac actgtttgtt acagatgaaa agacaactag
     2341 agatatagct agacattaca tatatgccta taaaaaaggt ttgaaaagtt tatattacac
     2401 aagaacaaag atgacaagag atactcatga gtgtctagtg tgttcagtat agggagagaa
     2461 atatatgaca tttaatttac ataaaataca taatgcagtt aactggaata gagaagaaga
     2521 tggatttaca caagcttttt gggaacaaaa tgtaaaacaa ttttggcttc ctgaagaaat
     2581 atctgtttct aaagacataa aggtatggaa tgaactaagt aataaagaaa aggaattata
     2641 taaaaaagta ttgggtggtt taacattact ggatacaaaa caggggaata atggaatacc
     2701 atctatgatg agtttaacag aaaacctaca aagaaaagct gtcttgtctt ttatggggac
     2761 tatggaagaa atacatgcaa aaagttattc ttctatattt atgactctac tttctaattt
     2821 agaaatagat gaattatttg aatggataga aacagaacca acattacaaa gaaaggctga
     2881 tttagtttta gctcaatacg aaaatactac aaatcaagag ggattatacc tatcaatggt
     2941 tacaagtgta tttttagaga gtttcttgtt ttattcagga ttcttttatc cattgtatct
     3001 atctggtcaa ggaaagatgg tagctagtgg agaaataata tcgcttattc ttagagatga
     3061 gtctttacat ggtaagtata taggattgct agcacaagaa atatacgatt catttgataa
     3121 aatggataag gagatgttag aagagaagat gtattctata ctttatagcc ttatggagaa
     3181 tgagatagag tatacaaatg ttatatacag agaaagtggg cttgaaaaag aggttgtgaa
     3241 ctttttaaaa tacaatgcaa atagagcttt agaaaatcta gggtttgaga agctatatac
     3301 tgtagatgct ataaatccaa ttgtattaaa tggtcttagc actgaaacca aaacacatga
     3361 tttcttttca actaagggta atggatacca aaaaggagtg tatgaggaat tagaggatga
     3421 agattttata atataattta taattatgac acactgttgt cacttttagc gtgataatat
     3481 caatgtataa aatatatatg gaggtatttt tatgaaatat gaatggagaa aaagagataa
     3541 agagttatat ttacctaaaa agaatcctgt aatagttgaa ataccagaac agaaatttat
     3601 catgttaaaa gggagtggag acccaaattc taaagaattt actgaagcaa taggagtact
     3661 ttattctttg gcatatgcaa taaaaacaat gcctaaaaaa ggaattgtac cagaagggta
     3721 ttttgactat acggtttttc ctttagaagg tatatgggat aagggagaaa aaagtaaagg
     3781 cttagatgta cttattaaag aagatttaat ttacactata atgatacgtc agccagattt
     3841 tgttacacaa gacctgttgg ataaagctac tgatatagta aagaagaaaa aacctcataa
     3901 tcttcttgaa aaagttagat ttgggtctat taatgaaggg ctatgtgttc aaatgcttca
     3961 tataggttca tatgataatg aaccagaaag ctttgacatt atgaacgaat tttgcaaaaa
     4021 aaataaactt ataagaaaat cttacacaca tcgtgaaatt tatataagtg atgctagaaa
     4081 agtatcgcct gaatcattga agacggtttt acgttttgaa gtagaaaggg aagtataaat
     4141 attttgaata atttataaga gttataaaaa gtgaagtctg ttgagacttc attttttgat
     4201 gcaattttaa aattaaattt gatgtttcat aatattttaa aaactttcta cttttattag
     4261 tgtgaataac tgttgctatt aaataataat actaattata taagtattat tatataatat
     4321 ttatagatta ttatataagt agattattag taagatgtat gcattattaa ttaaaataaa
     4381 ttacatatac tatttaatat ttcatttaaa atattaaaaa atgtagataa aaattgtttt
     4441 attaagaaaa aattaatata ttgacaaaat ttaagtgctc atttaaacta ttaattgata
     4501 ataatattca atattaaata ataggaggcg ttatgaataa atatcttcta ataaatccag
     4561 tggcagaaag aatgtatgga gaaaagtttc accttattaa agcaaatttg ataaaaaagg
     4621 ggtacataat agcagaatgt gaaccacagt tagaatatgt aaaaaagcag tacaaagagt
     4681 atacaaaaaa gactaaaaat acaatgttgg attgtagatg tccagaaagt ataagtcttt
     4741 taaaaagaaa taatttgata aatgattttg aagtacctat gatagaacca atattagtaa
     4801 ggacttgtag ggttttatac gataaatata taaaaagtaa agatgatatg ttggtagtaa
     4861 cctgtccatg tacacaatta agagactttg caagtgaaaa gtttaaggat aagggtaatg
     4921 ctatgtttta tacatggaaa gagtttgctg aacaagaagg tgtaaaatca cttggaaaaa
     4981 tagatgaatc accaattcca ttaggatatt tcaaagatac ttttgaaaaa gttcttgaag
     5041 tatcaagtga agacgaaata ttaagtgaaa tacaaaaaat aagaaatggc tttgaagata
     5101 agtatgacat agtagaaatg ttttattgta aagatggttg taataatgga gatggtttgt
     5161 aaatggtcaa taaatttaat aataagtttt taaggctagt aaagagtgtt ttgatatttt
     5221 ttataattct tttattatgg aaaattacaa attatcttgg tatttggagt gattatatat
     5281 taccatcacc cgaaaaggta tatagtacat ttttaaatat gataagtgat ggttcaatat
     5341 ttattaatgt atatgcaagt atgaaaagag ttttgatagg ttttgcaata agtactgcta
     5401 taggtgtgcc tttagggata ttctttggaa tttatagtgg agtatacgaa tactttaaat
     5461 ctctaataaa ttttttaagg aatacacctc cccttgcttt aataccaatg cttatattat
     5521 ggtttggtat aggagaggaa tcaaaaatta taattatagt tttagcatcc tttttcccaa
     5581 tctttacaag cacattgaaa gggattaaaa attgtgactc aaaactgatt gaagtgggta
     5641 gagtatttga gttttcaaag cttcagataa tatttaaaat aataatacca aatgccattc
     5701 ttgatattgc tgttgggtta aagttagctc ttggatatag ctttagagca attattgggg
     5761 cagaacttgt agcagcttca tctggtcttg gatacttgat atctgatgga aaagaaatgt
     5821 ctagaacaga tgttgttatt gtaggtataa tcgtaattgg acttttagga ataattacag
     5881 attacatatt ttcgattatt gtgaagaagg taagtaaggg aaagatggtg gaagcatatg
     5941 aataatttag tatgtgactc tggatatata attagaaatt tatataaaag ttataaagtt
     6001 gacaatgaag aacatgtagt tcttagtgat atctctttaa acatagatag aaaccatata
     6061 acagtaattt taggtgaaag tggttgtgga aagactacct tgcttagagc aattgcatca
     6121 cttgaaggta taaatagtgg tgaaattagg tttattaatg ataataaaga gtatagacct
     6181 aatgttggtt ttgtttttca ggagagcaga ttaatgcctt ggttaaacgt aagtgaaaat
     6241 atagcttttc ataatcaaag aaaaaatact attttaaaca aggtgcttag aaaatttaaa
     6301 cacaataaaa gaattagcag taaaattata aatgataatt ttagaagctt agaaacaaat
     6361 gataatttga acaaatcaaa taaatataat aggaaaagta atattgatgt agaaaaatat
     6421 cttaagatga tgaacttgca gaaatttaaa aactcttatc caaatgaatt gtcaggaggt
     6481 atggcacaga gagtaagcat agctagagct ctttctttca atccagatat gttgcttatg
     6541 gacgagcctt tttcggcatt agattatttt acaaggattg atatgcaaaa tgaagttata
     6601 agaatacata agtcaactaa taaaggcgtg atatttgtaa ctcatgatat tgatgaagcg
     6661 ttaaagatag gcaagaaaat agttgttttc acagacgaaa gaaaagtaaa agaatttaat
     6721 atagaagatg gttataatag agatttaact tcagattact atattaaatt aaaaaaagat
     6781 attttaatga caatgaaatc aaataaaggc atttaaagaa attaataata ttttaaggag
     6841 taaagtaaat gaaattaaaa aagctattga taggtataac gatattaaca cttgcaacag
     6901 catctttagc tggatgtgct aaaaaaaata atggagaaaa actttcagaa ataaatttaa
     6961 cttatgtaaa atcaccatta aatgttcctt ctataattca aaaacaggat gatttatttg
     7021 gaaaggaatt taaaaaggat aatataaaag ttaacttcca cgaaattact acaggtccag
     7081 agcaaacaca agctttagct gctggagaaa tagacttctt acatgcacta ggtggtacat
     7141 cagctttaat agctgcatct aatggagtag agttaaagat attaaacact tatagcaggt
     7201 caccaaaagg atttatgatt ttgactaata ataattcaat aaaatcagca gcagatttag
     7261 taggtaagaa ggtggcaggc ccaaaaggga ctatattaca tcaggtattg atttcagcac
     7321 ttgataaaga aggattaagt atggatgatg tggaatttgt taatatgggt attccagagg
     7381 catcagcagc tctttctgat ggcagcgtag atgctgcatt gattgctggt ccagcagctc
     7441 ttaaagctat gaagtcaggt agtaaacttg tagcaaatgg tgaaggttta gtagatggta
     7501 taatagtaac tgctgttagc acagactttg cagagaaaca tccagaaata gttgaaagat
     7561 ttatgaaggt tgaaaaggaa actttagaat atgtaaataa taattttgat gaagctatgg
     7621 aaaaagtagc taaagaagtt gatttaagtc ttgaagaaac taaagaatta tatgcttggt
     7681 atgattttag tttagatata acagataaag acatatcttc gcttgaagat acacaagact
     7741 tcttaataaa aaataagtta caagagaaaa aagtgaacat aaaagaatta atctataatg
     7801 ctaagtagaa actatgataa aataatacta agtaagaaat ataataaaag tttaagttac
     7861 cttattaagg taactttttc tattttgaat tagtaatttc aatttatttt agaatgatta
     7921 gttgataata atgattaatt aatcacaaaa agaacttttt tatagagttt tattaagatg
     7981 tctaataata tggttatact tatgaagtca gtaattgtga agttagtaat atgattaaat
     8041 ttatatattg taaaaagatt ataatatatt aaaattggac tgttatataa tgagtaaata
     8101 ggtatattag gaggaattat atgagttata aagtatatat tgtggaagat gatatatcaa
     8161 taagtacatt acttaaagat tatattgaga aatatggatt tgaagtgatt gttgaagaaa
     8221 actttgatga tataatgatt acatttgaaa gttttaagcc acacttagtt ctattagatg
     8281 taaatttacc taaatttgat ggtttctact ggtgtagatg tattaggcaa cagtcgaatt
     8341 tacctataat ttttatatca gctagagatg gtaatgtaga ccaaattaga gctttagaaa
     8401 gtggagcaga tgattatata acaaaacctt tttactatga tgtagtaatg gcaaaaataa
     8461 agagtcatat aagaagggta tatggtgaat attcacctaa aatagacgaa agaatagtgg
     8521 agctagatgg attaaagttt tatccagaaa ggttagaact agaattaaat ggtgagaatc
     8581 taattataac taaaaaagaa ggtatactga ttgaatgtct tatcaagaga tacccaaaag
     8641 tagtaagtag agattatctt ttagaaaaga tatgggatga tattgagttt gtagaggaaa
     8701 atactttaaa tgtaaatgtt agcaggataa ggaaaagatt tgaggaatta ggtatagata
     8761 atgctgttca gactgtccga gggttaggat ataaattaaa taagaattgg taaggagaaa
     8821 gtatgaagtt atttttaaaa gattataaag gttatatttt tatatattat ataggcatag
     8881 ttatgacaat tatttactgt aatcttatga agtttattag ttttggagag agtttatata
     8941 ttcttttatt tagttctttt atattgttat gttttttaat atataagtat tataaaaata
     9001 gagaagtata taatctgttt gaaaaaggaa tacattcttt ggaggagtca tttttgtatt
     9061 taggaagctc attcttgggg agaaatatat cagttatttt aaaacaacaa cataaattgt
     9121 atcaatcaat gatacaagag cataaaaaga tacatagtga acatttaact ttcatcaacc
     9181 aatggattca tcagatgaaa acaccaatat cagttataag ccttcagctt caagattatg
     9241 agggagaaga agtaactgaa aatataagaa aagaaataga tagattaaat aaagggttag
     9301 atatggctat gcattttgca agggcagata attttcagaa agactttata gttgaaaaag
     9361 ccaaattaaa tgaaattgta tctagtattg taaatgaaga aaaaaggtta tttataaaaa
     9421 gtggaatgat tcctaagatt caagtagaca aatttactta tgtttataca gatgtaaaat
     9481 ggatgaaatt tataataggt caattaatta caaatggagt gaaatattct aaagattata
     9541 gtaactacct aactataaaa tctgaagaaa ataataaata tgtaatttta agtataatag
     9601 atgaaggaat tggaattgtt tctaaagatt taaaaagggt atttgaccca ttttttacag
     9661 gagaaaatgg tcgtaagttt ggagaatcta caggtatggg tctttatcta gtaaaaaagg
     9721 tatgtgatga tttaggtcat aaagtaagta taaaatcaga agtaggaaaa ggtacagaag
     9781 tttcaatcta ctttaataaa gatgtaaata gtttaataaa ggtgtaaatt atctgttaca
     9841 atagggtttt tagttaattg tactttttta ttatacaatt tattaaatga aatattcata
     9901 gttaaataat caaaatttaa gagagtttat ttgcaaacct tacatagatg taaggtttgt
     9961 gtaatgtttg cagatagtta aattttctaa tctaaattac aattaatata agaaataaca
    10021 taagagaaat aacataaaaa aagaaatttt gtaaaaaagg aaggatgttt attgatgagt
    10081 ttagatgtta tttttatatt gaaatcagtc ataatagcta tagtagaggg gcttacagaa
    10141 tttattccag tttcatctac aggtcatatg attttggttg gtaatttaat agattttaag
    10201 gggcaatttg ctgaaatgtt tgaggttgta atacaacttg gggctatatt agcagtagta
    10261 gtactttatt ggaaaaagat aaaagatagc gttatagaat tttttaaatt tatatttact
    10321 ggtggaaaag aaggaaagat tggttttaga tttggaatga atgtaattat aggttgtata
    10381 ccatttgcga ttataggagt tctattttac gataatataa aatcattgtt taatttacaa
    10441 tctgtaatta taggatttat agtgggtggg attttattat tagtagtaga aactttgttt
    10501 agaaaaaaga atcattccac agataatata gataaaatta ctccaataca agctttaaag
    10561 gttgggacat tacaagtttt gtctgcatgg ccaggtatgt caaggagtgc atctactatt
    10621 atgggtggat ggatagcagg acttaattct cctacagcag ctgagttttc tttcttttta
    10681 gcagtaccag ctatggtggc ttcttcagga aaagatttat ttgaatttga ttattcaata
    10741 atgacaccaa cattgtggat tgctttggta gttgggttta tagtagcttt tattgtatca
    10801 ataatagtta tggaaaaatt cgtgaatttt ttaaagaaaa aaccaatgag agtgtttgct
    10861 gtatacagaa taatcatggg tgtagtactt gcagtattag cttttactaa cataattagt
    10921 gtatagaaaa aatagattta ttatctataa aaattaaact aagataaatt ataattaaca
    10981 attatatata tgttatttca aaaattaaat taggaaggat agttaaataa tgaaaatttt
    11041 aactgtaaat aatttaagta aagtatatgg taaaaaaata attttcaacg cattaaatga
    11101 cataaatttt agtatagaag atggagaatt tgtaggaatt atgggtccat ctggtagtgg
    11161 aaaaactact cttttaaata tgatttctac tattgacaag ccaactacag gaactatgga
    11221 attaaagggc aagaatcctt tattgttaag aggtgaggaa cttgcattat ttagaagaag
    11281 agagttaggt tttgtatttc aggattttaa tttattggat acattaacta taggagaaaa
    11341 tatagtattg ccattgacat tagataaggt cagtgtaaaa gagcaagatg agagattgaa
    11401 tgaagtatct acaatattag gtataaaaga tttattagga aaaagaactt ttgaagtatc
    11461 aggtggtcaa gctcagagga ctgctattgc tagagcattg attaacaatc catcaatact
    11521 tttagcagat gaaccaacag gtaatttaga ttcaaagtca tcaaaggttg taatggagct
    11581 atttcaaaag ataaataaag aaaataaagt tactactatg atggtaactc atgacccatt
    11641 agcagcgagc tactgtagta gaatcttatt cataaaagat gggtctatat ataatgaaat
    11701 atataagggt agtagtagag agcagtttta tcaagagata atggatgtac ttacattatt
    11761 gggaggcgat aactaatgaa tataaaacag tttgctatta acaatatttc aagaaatatt
    11821 aaagcgtatt taggatactt agcaagtatt gttatatcat cttcactgct attttctttt
    11881 attatgttta caagtcatcc agatttagat gtatctcaat ttcctgatta tttaaaagaa
    11941 ggtctcaaga tgagtaagat aattgcatat ctattcttat tcttctttgt gttttattca
    12001 gttagtgtgt ttcttaaaag tagatataaa gaatttggaa tactttatat tacaggtata
    12061 tcaaagcgcc aagtaatgaa attaatcttt atagaaaata tgataataaa tatagtatct
    12121 tctgtaattg gaataataat aggcttaatc tttgcaaaga tatttttagt ggttatgtct
    12181 acttttttag aattatcgcc attaaaattc tatataccat ttaatgcgat attgtctact
    12241 ttaatctatt ttatgatttt agctgtatta acatcaatat ttacttcttt tatagttaaa
    12301 gaaaatcagg tgttgaagtt acttaaaggt acaaaaactc caaaatcaga gcctaaaaca
    12361 tcactaatgc ttgctatatt aagtatttgt ttgtttataa tagcgtatta taatgctatt
    12421 acctcaaccg accaatatac aacatatctg cgcttagttc cagttacatc ccttactata
    12481 gtagcaactt acttgttctt ttctcaattt agtgtatttt ttatgaagaa gctaaaacta
    12541 aacaagagat tttatagaaa aaatacaaat atgctttgga tttcgaactt gatttataag
    12601 gtaaaggata atgctagaat atttttcttg ataactataa cttctgcagt agcatttact
    12661 gctataggta cagtttattc cttttggaaa gatgtagagc gtcaaattaa tttaatatat
    12721 cctaacacaa tatattattc aacaatgaca ttgcataatg accctaaaaa gcctgatagc
    12781 aaagaaaaag acaaggaaag aatgatgttt atagaaaata atctaaaaaa agagaatgta
    12841 gactatacaa ggactaatgg tgaatttaaa actgtatttc ccaagaaaag tgattttaat
    12901 gtcagaatta tgaaagaatc taagtactta gaaattacag aaactatagg ggttaagcct
    12961 ataagttttg aagataatga atgtatatca ttattatcat caagtttacc aggaaataat
    13021 aacgtcaaag aaaatattat tgtagggaat agaggtttaa aggtggcaaa acaagtggaa
    13081 gaatgtgtta tgccagcata ttacaaaaat atgtatgtgg taaaggataa tttttatgat
    13141 agtatagaat caaaatttat agtggataga tttactgcat ttgaagtaga ggattcaagt
    13201 aaaactatag agatatgcag agaatttgaa aataaattta ataatgagtc agggatgcaa
    13261 ccatatttgt ttttctctaa agaattaatg ataggaactg ggaaattaat gtattctaca
    13321 tttatatttt tagcaatatt tataggttta attttctttg ttactacaag tagtttttta
    13381 tataataaat tttatatgga ttgccaagtt gataagaaaa agtatgaaca gttaaataaa
    13441 ttgggtatga catataatga gattaaaaaa gcttcaacaa ttgagattgg aatagtattt
    13501 ttgtttcctt atatagtggc tgttatacat tcaatatttg cacttactgc actgaaaaat
    13561 tcttttgata tagaagtagc atcatcagct gttttggtaa tgggaagctt gttcttggtt
    13621 caaatagttt attttttaat tgtaagaaat aattatt
//
LOCUS       DAGSHT010000089        13606 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGSHT010000089 DAGSHT010000000
VERSION     DAGSHT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.091"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            267..461
                     /locus_tag="MJB72_002216"
     CDS             267..461
                     /locus_tag="MJB72_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263321.1"
                     /translation="MLNKLIFNGRIVMKIFSFLLFVIGICLCIAGFIGHYPVFVGTTL
                     SFLAIAMIAVRQVKTKKKMK"
     gene            complement(748..1452)
                     /locus_tag="MJB72_002217"
     CDS             complement(748..1452)
                     /locus_tag="MJB72_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263322.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1443..3050)
                     /locus_tag="MJB72_002218"
     CDS             complement(1443..3050)
                     /locus_tag="MJB72_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBY8263323.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3304..4482)
                     /locus_tag="MJB72_002219"
     CDS             complement(3304..4482)
                     /locus_tag="MJB72_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263324.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4708..5202)
                     /locus_tag="MJB72_002220"
     CDS             complement(4708..5202)
                     /locus_tag="MJB72_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY8263325.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5203..5964)
                     /locus_tag="MJB72_002221"
     CDS             complement(5203..5964)
                     /locus_tag="MJB72_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY8263326.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6686..9520
                     /locus_tag="MJB72_002222"
     CDS             6686..9520
                     /locus_tag="MJB72_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8263327.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     YSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9563..10807
                     /locus_tag="MJB72_002223"
     CDS             9563..10807
                     /locus_tag="MJB72_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY8263328.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(11222..12964)
                     /locus_tag="MJB72_002224"
     CDS             complement(11222..12964)
                     /locus_tag="MJB72_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263329.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(12948..>13606)
                     /locus_tag="MJB72_002225"
     CDS             complement(12948..>13606)
                     /locus_tag="MJB72_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263330.1"
                     /translation="KDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGSGKSTLL
                     HCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINVYDNIVL
                     PVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRPSVIFAD
                     EPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGKIDEKNY
                     "
BASE COUNT         4813 a         2217 c         1604 g         4972 t
ORIGIN      
        1 aaagacaaaa agggcaattt ttttatatca aaaaaaatgt taaattgata ttatccatat
       61 agatgctata tttaacaatt ctacttactt cccttacact tattagcata tagttattaa
      121 gaatttgttg caaaacaaca cttagcacac tttaaaaagt gtggtctaat aagtaatcaa
      181 cgaagaagac ataataaagc ataaaatatg atatactttt ccacgtcatc aaaactgagg
      241 attttattat taatatatgc taaaatatgc ttaataaatt aatttttaat gggagaatag
      301 ttatgaaaat atttagtttc ttgttgtttg tgataggaat atgtttatgt attgctggat
      361 ttataggaca ttacccagta tttgttggta caacattgag ttttcttgca attgcaatga
      421 tagctgtaag acaagtcaaa actaagaaaa aaatgaaata ggcaaatcct aactttacaa
      481 gttaatatta cacaagtaat tagtctaaaa ttggatactt tttctattac agaaatacat
      541 catccacttt ttattctctt aaaaaaataa aatagctatg taaattcaag attagataga
      601 ttaactctct atccatttca ttgtaaaata tacatagcta tttgtattcg atttaaaata
      661 cttagatttt cttacataac aaataatttc tatctgatta ttactcatat tcatttagta
      721 atcactcagc aactaatcat ttagtatcta ttcatccgcc atacggtaac ctaccccaac
      781 ctcagtcaaa atatattctg gctgagcagg attcttttca attttccttc ttatacttgc
      841 cataaaaact ctaagcgact gattttcact ttcatagtaa tttccccaaa ctttatctat
      901 tataaactta tgggttaaga ctcttcctga atacttagac atcaactcaa taatcttata
      961 ttcgattgga gttaaatgta tttcttcacc attaattgac acaatatgat tttcataatc
     1021 tatctttagg tttttaactt caaaagactt tggagcatca ttatttatat tatcctgttg
     1081 agctttattt ctaagagcca cacgtactct agccaaaagc tctgttacac taaaaggttt
     1141 agttaaataa tcgtctgctc ctccatctaa agcttccacc ttatccctct catgctctct
     1201 cgctgaaaca acaattattt tattagtctt tgcacatgcc ctaactcgtc ctattacctc
     1261 aatcccatca atatctggta atcctaaatc caaaattatc aaatctgggt caagagaatg
     1321 tataagtgat atagctgttc ctccattatc tgtctctaca cacctataat tttgagtttc
     1381 aagagatact tttataaatt tacaaattgg cttatcatct tctacaatta gaactaatgg
     1441 ctttatttcc acttgtcttt cccctcctca tcatacatag gtatgctaaa tctaaatgta
     1501 gctcctccag aaggattatt aaaggcgaca atttctccac catgagcttc tactatagat
     1561 ttacatatct ccaatccaag accaatacct cttctttcaa gatttctaga cttattctta
     1621 gtataaaacc tattaaaaat attcccgata tcttcatttg gtattccttt tccattatct
     1681 gcaacttcaa atagtacttg atttgactct ttttttacag ttagttctat cttagaattt
     1741 ttaggtgtat gccttatagc attatcaatt agatttacta acacttgttc tattaataat
     1801 acatccacat gaacgattat tatctcatct ggaatatttg tttttatgtc tctactattg
     1861 gcaaactttt tgactcttaa tatagattca gctattattt catcaacaac ctctggtcgt
     1921 ttttcaatat ccaattttcc ttcatctatt ctggtcatac taagtatatt ctcaacagaa
     1981 tgagttagcc aactcgtatc ttcatatata tttttcaata attctactct agtttcttta
     2041 tctatcacat catcattttc aagtattgtt gaagatgaac ctaatatact tgtaagtgga
     2101 gttcttaaat catgagaaat tgacctaagc aagtttcctc ttaatctctc tctctctgcc
     2161 tcaagatttg cttttttatt tttctcaaat aaattttctc tatcaattgc caacgcaacc
     2221 tgtgttgaaa ctgcttctaa tagtatcttt tcattttctg taataatatc tatcttaaaa
     2281 cacgcaacac ctataacacc aagaacattt gattcttgac cttttatagg gaaataatat
     2341 gcactgctgt ttttacaata ttttgtccca attccaactg ggttacctgt cctaaaagct
     2401 tcttttatag cttccttttc taatactgaa tggaatatat tctcacttcc atcattgtta
     2461 caaatataaa tacttggctt acctagatta ttattctcat ctgcaatagt cactattaca
     2521 gttctattta atatatttac taaattagac ccacaaaatt caataatttg atttttattt
     2581 tttgctttaa gcaacgtttt gttattctca tagagcaatc ttatcatttt ttctctctta
     2641 tgagaaagtc tcacttctct ttttatccta gaagttaaag tacttgttat aattgctgcc
     2701 attagcataa tacaaaaggt cataggataa tcttttctat aagctaaaaa agtatataat
     2761 ggatgcgtaa agaaaaaatt aaatgatagc actcctatta ctgaagaaag tattccaaaa
     2821 aaatatccat aggttatact tgtagcaaat agtacaccta atatgtatac tagtataata
     2881 ttagattcat taaaacctac cttttcaaaa actactgata ttccagtagc aattgtcata
     2941 accaatatag tttttatgaa gtccttaata attatctttt tactgagata gccatcttca
     3001 tcataatcat actcttttaa gtcttcatgt tgtagtcgtt cctttttcat ttactacaat
     3061 tcctccttat tgttactgtt taaattggct ctattttata taatacaatt ttatcataat
     3121 aaattcaatc aatacattta attaatgtac ttaaattact aaaaggtgct gtcttaaaat
     3181 gaaaataaat ctctatttca aaacagcacc tatatattta aattactaat ttagttttta
     3241 catacttaaa tcaatagttt tattcaattt cataaattat aatacttcaa ttataaaaca
     3301 tttctatcca acaagcataa atataaagtg ggcacatata cctgcacata aaccacctat
     3361 agtatgcgaa agtatcgctt taccagccaa tggtcttgca tctaaagcat ccatcatacc
     3421 tatgtgtgta cttaaatatc cactccaaca catacccatt gctgtgaata ctgctatatc
     3481 attaggagtt atagcgcctg attttataaa ctctggtact aaagatattg ctgctccaac
     3541 agctcctaat gcagttacag ggaatgctat tgcctctggt gatgtgaatc caaataatgg
     3601 ttctattatg aagctaatct tatcaccaat agcaggtaat aattttatac cttcatatgc
     3661 agctcctgtg taaacagctt gacctgtagc agggtctgta gatggtccaa aagttaataa
     3721 cataactaat gtacaaacta ctaaaactcc tggtattata gccataccca tttcaacacc
     3781 taacttacca ccttcaagta tagcatccaa tgttctttgg aatacattac catctcttat
     3841 ttctctaaac tcttcaagtt ttgcaccttt atcatttatt tgcttttcag tatcattttt
     3901 agggtcataa ttataatact ttttagtaaa agtcaacata attctaacac taataatact
     3961 acctattatt gccgcaacat tacctattat agctggtagt acatattctt taccttgagc
     4021 tatcataaaa gttgttacaa taagtcccat accaaaggct gttcctaaat tacataaagc
     4081 aggtacttga tacttcttaa aatattgtgt aaatgttttg tctttagcaa atggtataat
     4141 agctggatta tcagataaat atgttgcaac tactcctgct atactagcac caggtaagcc
     4201 ccatataggt ctcataagac ccttaaatat cttattaact aaagatatta ctccaaactc
     4261 tgaaagcaat gcactaagtg cacctgcaag aactgccata gccattatta aaaatactgt
     4321 gtctaataaa agcgcatgtg ctgtactcat tattacttta aacatcattc ctgcacccat
     4381 gatgcttcct acatatccaa atcctaccgc caatagtaca ataaatacga aggtttccat
     4441 acttactgcc ttgactcttc tcttatccga cagcttttcc atcttgaaat cttccccctt
     4501 ttaattttaa attaaggttc ctaacattgg tacaaatttt ttggtacaaa ccgagttcta
     4561 aaacttacac tcgtaataca agttgtcagt tttttgaaat aaaaaaaaat tacgtttata
     4621 gaataagaat atcctcttta atttggatac tcttattcta ttttatgtta ttatattatc
     4681 atccttttgt ttaaaagtca attttttcta tttttgttgt ttaattttat ttatactctt
     4741 tttatatttt tcattttctt ttatctttgt aagtactcca tataagttga cttttctaaa
     4801 tacagtagtt ataaatacca atcctaatgc aattgaactt gcacacgata atgtactcac
     4861 cccatattct acagctttta tataattatt ggtcaaaaca gaatacatag tattatatat
     4921 tccataacca ggtactaata ttattaaaca gcaaacactt aatgtagttg ctggagtcat
     4981 ataaattctt gcacataact cacagtatat cgcaaaacaa actgtagcta taaaaaaaca
     5041 taagttttct gttactccca tatttaaaag aaatttatat gaaaaccaag ctatacttcc
     5101 acctattcct gctagtatta agttttttcc tttcatatta aatagcactc caaagcttaa
     5161 ggcagtaaaa aaagctgcta ttacttctat aaacaaatgc acttatatcc cccctaactt
     5221 gaagtacaat ccaagtacca agaacattcc tactgctata gatacaccta caaaaaatac
     5281 ttctgctaat tttgcaagac cagaaatcaa ctgtccatct aacaaatctc ttaatgcgtt
     5341 agtcaaagca agcccgggaa ctaagagcat tattgctcct gctattaaat ggtctaacgt
     5401 ctttatgaat ccgagcttta aaattaaaat tgaacaagaa gctataataa ctgctccaat
     5461 tgcatttaca aaaaagtcat taagggaaga tttacttaaa tacataataa caaacttagt
     5521 caaaatccca acagtaaatg cagctataaa ttcttccaca tttcctccaa ataatatcga
     5581 aaaagttgct gctgcaatac ctgcaaaaaa tattgttatt aaatccgagt aatagttatc
     5641 ttcacttatt tcatctaaaa ttttctcaca taattcaata tctaaattat ttatacttat
     5701 tgctcttgat aatgagttaa cttcatgaac caagtttagg tctactcttc ttgagcttat
     5761 tcttttaatt gctgaatgta atttcccatc tacaaataca gatgccatta cagctgttgg
     5821 agttgcaaat acatctacgt ggtctattcc aaaagattga catattctag ctatcgtttc
     5881 ttcaactcta taggtttctc ctccactttg caacatagct ttacctgcat ttgaggaaaa
     5941 gtttaaaatt ctatctatat ccacaaaaca tatctcccat tcttttatag tattattcat
     6001 attttaatac aagatattat ttatttaaac tattttaaat aattcatatc caatatttac
     6061 attttatgaa aaaacttgtt ttttatgctt gtcaaaatca aattttaagc ttgtttacac
     6121 cataaaatgg ctgaattaat atttgacaaa cactttcaac catttatata accttctaaa
     6181 ttgtataact ttttactaca aataaaatta tatctctttt aattctttct cttgagtatt
     6241 tagcttatag taacttcata tttatatatg tacaatataa aacattattt ttatgataca
     6301 aaatataatt tgtaaatgta tatatgtatt aactgtgtat atataaatta ttcttaacct
     6361 tatcagtttt ttcatatatc ttcatctata ccgaccatat attaattaca ttgaccatat
     6421 attaataaat gatattacat agacttaagt acattaaaaa acttaaattt agttatttaa
     6481 caacataata ctctaattta atctataaaa atccatacct aatataatgc aactcttatt
     6541 ttaacaccat ctattacaca aaacaacata ttaaaaagta aaatacaaaa tatccactat
     6601 aaaccttatt ttaatactga atatatatta taatatattt aatgtagctt aaatatagtt
     6661 taaacataaa gaaaggaaac ggtatatgct aagaaaagat aaagttctat tatctctaca
     6721 taaattatgt gaaaatatga ctttaaaaga ccttcaatca gataaaacag gattcactac
     6781 aatagaaatt gctaaccatt catctcttga tagaagtaat accagtaaag aattaaataa
     6841 cctatacaat gaaaaaaaag ttctgaaaat atctggtaag ccaattttat ttctacatgc
     6901 tagtattata gaaaatttaa ttggccgtac tttgaacagt gatgaatttt ctataaataa
     6961 ttgtaatgag ctacttaaat caagtaaaaa ttactcagaa gatatttttt caacattact
     7021 tggacatgat tctagtctta aactacctat agaaaaagct aaatctgcta tactctatcc
     7081 tccaaaaggt cttaatacat tactcatagg acctactggt gttggaaaaa gtacatttgc
     7141 cgaaacaatg tacaaatatg ctgtagaaag taaaatcttt tcttctgaaa gcaaattcgt
     7201 agtatttaac tgtgctgaat atgcagaaaa tgctaactta ttattaagta atttatttgg
     7261 ttatgtcaaa ggttctttta ctggtgctaa taaggataaa ttcggcatag ttgcacaggc
     7321 tgatggaggc atattattct tagatgaaat acacagatta ccaccagaag gtcaagagat
     7381 gctttttctt ttgatggaca aaggcatata cagacgatta ggagaatctg acattgtgca
     7441 caaagctaat gtctttattg tgtgtgccac aacagaagat atacattctt ctctgcttgg
     7501 gactttttta agaagaatcc ctgttacaat caacctacct gctttacagg ataggtcttt
     7561 aaaagaaaga ttatctctta ttaaatactt ctttattata gaatcaaaat acaccaatgc
     7621 aactataaaa gttcacaaag atgtaattaa atcattactc ctttatgatt gttttggtaa
     7681 tataggtcag cttaaatctg acatacaact tatgagtgca agagccttct taaattacaa
     7741 aactggactt aaggataata tagaaataga tttatccctt gttcctgatt acatctatat
     7801 tggtctttta aaattgaatg aaaatcgtag taatctaaat aaattatcag aacttgatta
     7861 tatgcttttt tatgaattta atggtaataa cttagaagtt gaatatacaa aagaactatc
     7921 taaatacaat atagatacat caaacaatgg atttaaatac tctacagaga ttccttatct
     7981 ggaaaatgat ataaggagta tgtttataaa ttacatggaa aagtactcta caaaattact
     8041 ttcgatatct tcagtaactc attctgatta tacaagtaat gagattttta aagtaatcaa
     8101 tccaaagatt tactatgctg ttgaacattc tttaaaagag gctgaaaaaa tatcgagaaa
     8161 caaatacaca aaacaaactt atattgctct ttcaatgcat ataagtgcac ttatagaaaa
     8221 tataaatcaa gacacatata atcctaagta caatttatat aatgataaaa ttttcttaga
     8281 taaagattta aatatagcta aaatagtttt aagtataatt gaaaatgact tacaaacatc
     8341 tttacctaaa gatgaagtaa aatttatatc catgtttcta aatagcatgt atcttgaacc
     8401 aaacaatcaa tctgaaagta taggagtcat aatacttgct catggaaatt caacagcaag
     8461 cagtatgtgt gatgtagcaa attcattgct tggtacaaac cattgtaatg caattgacat
     8521 gcctcttgat gtaaaagtaa gtaccatact tgataaaact atagagttgg taaaagaatg
     8581 tgacgaaggt aagggtgtac ttcttttaac agatatgggc tctcttatag cttttggaga
     8641 acttatttca gaaaaaacaa aaatcaaaac tcgctcaatt gaaatggtat ctactccaat
     8701 agttttagaa gcagtaagaa aaagtacact accagaaatg actttggatg aattagctaa
     8761 ttccttaagc tctttaaatc catatattgg acgagcagca atcgattcaa cagaaataaa
     8821 ctatcctgaa aagtcaagag agtatgtaat aataactact tgtataacag gatatggttc
     8881 agctatgaaa gtagcagact ttataaattc ttcacttaaa aatattagta aatacaatat
     8941 taatttagtt cctcataata tagaaagttt taaaatgtat aagaataaat ttagtaatac
     9001 taaaattctg gcagttgttg gaagtgtaga ttttaatgta gctaacaccc cttttataca
     9061 catagaggaa ctactaaatg gatatggcct agatatgcta aacaaaataa tagaaggtac
     9121 attcaatgcc aatagtatta caaatataaa agaaacaaat ctatcataca acagtttaat
     9181 aataaacttt atgacccaaa acttagagct tttggactct gttaaattat tcaactatgt
     9241 caattcttcc ttatttacaa taatagaatt aatagatgaa aacttaaaga gcaaatttaa
     9301 gctaggatat atactacatt gctcttttat gatagaaaga tgttttaaaa atacaacttt
     9361 tccatataat aacattactg aattaattag taaaaatcat gaactatata gtttaatacg
     9421 tgaagccttt aagccaacag aagcttattt taatattttg ataccagacg aagaaattgc
     9481 ttatataata gacatgatta atgagtatct aaataaataa aatttatagc acttattttg
     9541 tgcatattaa ttaatatatg aaatggagga cgagattttg aaaggaaaaa taaaacagtt
     9601 aacaatactt gcattaattt ttatatttat aactcctgta tttgcatttg cagatactcc
     9661 accagtacca aattcttcaa gagcagcact acttatagac caggaaacta aaagaatatt
     9721 atttgaaaaa aacattgatg aaaaaatgcc attagcgagt ttaagtaaaa tgatgacctt
     9781 tttattggca atagaagcag ttgataaaaa tcaagttaaa gagactgata tggtgaaaat
     9841 agacaagtct acagcttcag taggaggttc tacttgtaaa cttaaagatg gtgatgaaat
     9901 atctctagga gagcttatgc agggtctaat gctagtatct ggaaatgatg ctgctatagc
     9961 catagctaaa catattggta aaactgaaaa gaattttgta aatatgatga acaagaaagc
    10021 agaagaaata ggaatgattg atacgtatta ttttaatcca aatgggcttc ctatatatac
    10081 tgacccagaa cataaagaac ctcctattga aaatatgtca actgctcacg atatagttac
    10141 acttggaaag tatatgtacg accattatga aaatcaagtt acaagaataa ccactatgca
    10201 agtatataat gatactaaaa aggattttac acattataac acaaatccct tacttgtttc
    10261 agtacctggt gttgatggaa taaagacagg atatacagat aatgctggat attgccttgc
    10321 attttctatg atggtaccta aagatgctaa aaacgaaaga aatcatagat tgataggtgt
    10381 tgtacttggt gatggtaaca agaaaaatag aatttcttct tctgctactt tattgaaata
    10441 tggtaaagat aatttccatt ctaaaaagat agcacataag ggtgatatta tagagactcc
    10501 ttgtgttgat ggtattgatg attttaagat aacagttaag gttgataaag atttatatgg
    10561 tgttgtttca gataacgaaa atatcaatcc taaggtagtg tttaaaaata tgaattatcc
    10621 tatccataag ggagatattg ttggtgttgc aaagtattat aatgactctg gtaaatttgt
    10681 tggaagtgtt gatgtaaaaa gtgaaagtaa tataggatgt attcctttga aggataagat
    10741 taagattaag gttgctaaga taaataaaaa acttgagatt aaaaattctg tttgttttaa
    10801 agcatagtaa agcatattat taaaagggta tgttaaattt ataaagactc cactttgtgg
    10861 agtctttttg ttttttacaa aaaattaaaa tagtggcaac aattgaggat gtgagggaga
    10921 gttgccacta ttttgtatag ggtattatat tatgttttta agctagtttt actatcttct
    10981 atgtttatat aataactctt gtgtgtgaat ttaattgata attaatatga aaattatgtg
    11041 aagttttgat ttatttgtaa tatattctac tactattata atttttctac tggatatact
    11101 aatcatttgt ttaacattat ctgagattga tttgcaaact tttatataac taaattagtt
    11161 atataactta tagattccat taaataccct aataatcata tatgtacata caaagtcatg
    11221 cttatgcaaa atctgcagtt tcaactaact tattatataa accatcttta tgtagaagtt
    11281 cagcatcatt tccagattct attactctac ctttatctaa tacatatata ttatcagcac
    11341 tcttaattgt tgaaagtcta tgagctatta taataactat cttatcttgt atgtactctt
    11401 tcaagttgtt caatatctct ttttgtgaaa tattatctag agctgaagtt gcttcgtcca
    11461 gaataagtac ctttttatta cttactaaag ctctagctat tgatatacgt tgtttttgac
    11521 ctcctgatat tttgacacct ttttctccta ctacttcgtc caatccctta tctagatttt
    11581 ttataaaatc atctaaacaa gtaacttcta atatctcatc taattcttta tcacttattt
    11641 tattatttaa tactatatta ttttttattg tgtcatcaaa tacaaattga tcttgagtta
    11701 ctatatcaat attatttctt agtgatttta aattaatatc ctttatatta acaccatcta
    11761 ttaatatatc accttcattt atatcccaaa gtcggtatat tagatttgtt attgttgatt
    11821 tgccacatcc acttccacca acaattcctg ttacttcccc tttctcaaat tgtaaattta
    11881 tatcatttaa aacaaggttt aaatcctcac catatctaaa agatacattt ttaaaatcta
    11941 ttttcttaat ttcactgtta tctattctat atccattatt atcaacttta atatctatct
    12001 tttcatctat tactgaaaat actctattga tagacacttt tgattgtgct attctagtat
    12061 tcgcccttac tatagctata catggtccaa ttaacattcc tgtatattgt tgaaatgcta
    12121 ttaactcccc catcgtcatc ttattattta ttattttata tcccccaatt ccatatatta
    12181 atattgttat caatcctgtt agtatttgag atattgctat attactactc ataatgatgt
    12241 tcaaattcaa atcttttttt atcaaatctc tctctctctt taaatactta ttaaagaaat
    12301 tcttttttga atttaatata acaatatcca taatattaga tacgtactct tgaatcaaat
    12361 ttgataaact gcctgaatca cttcttattt cagaaatttt cttagatata agttttgtca
    12421 ttttattttg agaaaatgtt aatatcaact gtaaaaataa aactactaaa aataaatcaa
    12481 actgcaaatt aattaagaaa attaacgata ttatagctgt aaacacattt acaataatat
    12541 caaatataat ctctgctcca aagttttcta tcataaatat atcactttcc ataatggata
    12601 aaatattacc tgttttctta cttgaataat atctaccaga aagttttgat atatggttaa
    12661 gcaactttac ttttagattt atagtaactt tattttttat tgaagtatat aaatatgata
    12721 atataacatc aaatgtagct gataatatcc ttataaaaat atatatcaat attaaattta
    12781 taagcagaaa aatattttta tttcctatcc catcatctat tatacatcta attagttgag
    12841 gtattacaat atttacgcca gcaattataa aaaccattat aaatgatact atcaacttag
    12901 tcttattctt atatactatt atatttagta ttctaaataa attattatta atagtttttt
    12961 tcatctattt ttccatcttt tatagttata attctatcag ctgtctttgc aatattatca
    13021 ttatgagtta tcataactag tgtttgtttg tactctttaa cacaccattc aagcatattc
    13081 ataacttcat ttgtagtctt actatctaag ttgccagtag gttcatctgc aaatattact
    13141 gacggtctat tggctaaagc tctagctatt gcaactcttt gttgttgacc tcctgatagt
    13201 tcattcggat atttctttat ttgtttttca atccccaact tattaattaa tctcataaca
    13261 tattctttat cttctttttt cccatcaagc aacacaggaa gtactatatt atcatataca
    13321 tttataactg gtattaaatt gtaactttga aatatgaagc caaattcctg tcttcttatt
    13381 tcagacagct tatcatcatt taatgtatat aaatctacat catctaagta cacattccct
    13441 aaagttggct tgtctaagcc tgcaacacag tgaagtagtg tacttttacc agaaccactt
    13501 gcacctacaa tagctgtaaa tttatgtggc tctatatcta aatcaatacc atcaagagct
    13561 ctaacttcat tctcatattt tccatatatt ttttttaaat ctttta
//
LOCUS       DAGSHT010000090        13594 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGSHT010000090 DAGSHT010000000
VERSION     DAGSHT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.092"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            186..1364
                     /locus_tag="MJB72_002226"
     CDS             186..1364
                     /locus_tag="MJB72_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8263331.1"
                     /translation="MKYHIFLIITTLLIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYRLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1714..2595
                     /locus_tag="MJB72_002227"
     CDS             1714..2595
                     /locus_tag="MJB72_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8263332.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2786..2953
                     /locus_tag="MJB72_002228"
                     /pseudo
     CDS             2786..2953
                     /locus_tag="MJB72_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3213..5780)
                     /locus_tag="MJB72_002229"
     CDS             complement(3213..5780)
                     /locus_tag="MJB72_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBY8263333.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5783..6388)
                     /locus_tag="MJB72_002230"
     CDS             complement(5783..6388)
                     /locus_tag="MJB72_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263334.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6762..8222)
                     /locus_tag="MJB72_002231"
     CDS             complement(6762..8222)
                     /locus_tag="MJB72_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBY8263335.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANVLVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8577..9155
                     /locus_tag="MJB72_002232"
     CDS             8577..9155
                     /locus_tag="MJB72_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263336.1"
                     /translation="MAKSFTEIEKDNIRKRLISECELNWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9446..10696
                     /locus_tag="MJB72_002233"
     CDS             9446..10696
                     /locus_tag="MJB72_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8263337.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILVVGFITLLIAFVSKITLRNENL"
     gene            10857..11426
                     /locus_tag="MJB72_002234"
     CDS             10857..11426
                     /locus_tag="MJB72_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY8263338.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(12048..13388)
                     /locus_tag="MJB72_002235"
     CDS             complement(12048..13388)
                     /locus_tag="MJB72_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY8263339.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         4745 a         2230 c         1768 g         4851 t
ORIGIN      
        1 atcatacata ctgtatatta agatataata ctttctaaat tttatataac acagacacat
       61 gcaataaaaa agaaggtatc cataataagc tctttgcatg tacctaaata aaaactgtaa
      121 aatagaatat ataaaatcta atctatttta agtaaataca atttcacttt aaaagaggat
      181 gtactatgaa ataccatata tttttaatca taactacttt attaataatc gaactatgta
      241 acatttacat atgcaaaata gcatcttttg gaaacaaaaa tataactata cataagaatt
      301 acaaatatat ttttttaata aaaccattaa ccttcttgat aataatattc acaaaattac
      361 cattaccact atattatata ttgttttaca tagtaagtca taaattgctc ttaagcagaa
      421 aaagttctac taacaaccat tttattccca atatggcagt aataagtttt tgtattttac
      481 accttattac tgtaagtaca atatcacttg ttaaaaatac aagtccagat ataatatttg
      541 caaatatgac ctataggtta attagtattt taatagcttc tattttagat tttatcttat
      601 ggtactctat gaataaaata aaagagcata tttatataat aactcaaaat caaatagagt
      661 tcagtctatt tttaaaattt atctggttca ctttaggata tatattattt gatagtatac
      721 aaattacatt tgctccacca agttctctaa cttcctactt tatattggga agtaacatat
      781 tattaatctt acaactaatt atattcatca atcatatata ttccattgaa aaaaatattt
      841 atttagaaaa agaacataat agactaaaaa tgataaaaca agaacaatta ctaaaagtaa
      901 atttattaaa gaaaaaagct aacattgatg aattaacatc agcatataat cgaaaatttg
      961 cttttgaaaa aatacagcat tacattgata acaatttatt attttcattg atatatatcg
     1021 atttggataa attaaagacg ataaatgata catatggaca tttagctggg gataactata
     1081 ttattaactt ctcaagtata gttattgaaa acttaggaat agatgacata tttgctagaa
     1141 taggaggaga tgagtttata gttattatgc ctaaatctaa tgaaaacacc acaaaagaat
     1201 ttatagagaa aataagtaaa attatggata ataagatatc ttttagctat ggtattgtag
     1261 aagtttctag cgaaaacaaa atgactgtag aagaaattat taatattgct gatattagaa
     1321 tgtacactaa taaacaaaat aatcataaaa ggaacaataa ctaaaatgaa tatttacatg
     1381 agcttacttg taatcttact atgcctttta tcttattcta tctatcatta taattttttt
     1441 aaatatctag acataatgat taataactct aaaaataaat acaaagtata tattgtatat
     1501 tttcttttta attatttaat ttttattatt tgtacttatt taaggttaca tctagttata
     1561 aattggacaa tttttatgtt aattttattt atgcaaatta aagtaactta caaactttcc
     1621 acaataaaaa acctttttta ttcgttaaac tgctccatca ttggtttagc aataaatata
     1681 ttttttaggt ctttaatgtc aatagtgtta aatatgccag catatttatt tgatagtaat
     1741 tctattacaa atatttacag agtattttct atattgctta gttttatagt tacaggatta
     1801 tatttcagtt ataaaaatca taaaaataat aagattaact ttgaggcagt tttagaggac
     1861 aaaaaaaact taattttctt actgagcctt atgtttggaa tgtacttgta tctatgtttg
     1921 agtttgtcaa tatactatac tgttggaaat gtattgattt taaaattata tggaatcaag
     1981 tctgccataa tagttttggt aggtaactat attgcagaaa gatatagctt tagaattact
     2041 caaataaaag gttatgatat taaaaatcaa atagaatatg tagaaagtgt aaagacagag
     2101 tatgaaatta ataaattgaa tgatattgta tatacagatt tactgacagg cttctacaat
     2161 agaccttttg taaatcaaaa actatcagag tggacatcaa atcactacaa atttacctta
     2221 tgttttactg atttaaatag cttaaaacct gtaaatgata aatttggaca ccaatttggt
     2281 gataaataca tatccactac tgctcaaata ataaaaaaac ttataaatgt agaaaattcc
     2341 ttactattta gatatggtgg agatgaattt ttattccttt tacagaatac aagttgtgta
     2401 gaagcagaaa aaataatgat taatataaat tatgaattat caaaattaag taatactgac
     2461 gagtatcctt atgctctttc cataagctat ggattagttg agtgggatgg aatatccgat
     2521 attgaaactt taatatcaaa ggcagattct ttgatgtatg aaaataagct taaaaataaa
     2581 tctactttaa actagctagt taagatcttc tacaaaatat atgttataag agagtactaa
     2641 gaatgtcaaa atatatagat tttgcaaata ttgaatacag tttactaatg aatgcactgg
     2701 atataagtgt tagtaaacac ctattagatg aacactttac tgttgtttgg gctaatgacc
     2761 gttattatga aatgttcggc tatacctagc taaaaagtta aaaatgacca ctgtagccga
     2821 gggaatagag accagttttc aaaaagattt cttgcaagaa atagactgtg atatgttaca
     2881 aggatacgtg ttctctagac ctttaccaat aaaagacttt gaaaaactta tgtttcaaaa
     2941 ctcctccaat taacaatata aaattgctta ttataagtga tattattgta tcaacaaata
     3001 aaggaattgt ctcaaaattg attttttcta atcaaatttt tattttgaga cattctcatt
     3061 tttttgcata agaaaatacc caccaagcta cggtttttct taccgctgtt cggtgggtat
     3121 tagtagtatt atgaaatctg aaaactttaa gtattactac ttccgctagc gcaatcacaa
     3181 aggtcgaaag cttcaaagcg aaaataggct ttttactcat caaacgtcac cgtacctgcc
     3241 aacccgtcca ccgttatcat ttgtccgctc ttaatagttc gtgtggcaat gtgtgtagcc
     3301 ataacggctg ggatgccata ttcacgtgcg acaatgcttg aatggctaag aggaccacca
     3361 atgtccgtta caatcgcact tgcagatgaa aataatggtg tccatgctgg agttgtggta
     3421 actgcaacca aaacacttcc aggttgaaag ctttcaaaat ctgctggact attcagtaca
     3481 caagctggag ccgtcacaac accagtactg gtaccaatgc ccgtcaacac aattttccca
     3541 tctttctgct tctgtggtgc ttgtgatata gtttttttgt ttttttctgg caacttggat
     3601 ggtgacacat aacccatgta ttttttaagt tcagcttttc gaacaggtat tatcccgttc
     3661 atatcagata gagacttgtt tttatccaac tgctctataa gtacctccaa ttccgatttt
     3721 gtgagccaat aaatatcatc taaatgtgaa atagctcctc ctcgtatgaa tcgttctgag
     3781 atttcctgaa acatacaacg aatcagagga tgtcccattc ccatcaaata aatagcattt
     3841 tcacgcatag gagaggtttc ctgtgcccaa tggagcaact taaaaaatag ttttttacgc
     3901 aatccaccta tgtgttgtaa aatctcttct tctgcatgtt ttctacgctt ttcaaacgtg
     3961 gtctggcgta aaaaaggact ttcccctttt ccttccacaa atgtttttac ggattcaaaa
     4021 gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat caaactcata agcggtacga
     4081 ccaaactctt taaagtactt attgattcga tttttccatt ctatccatac ttcctgtgaa
     4141 actccaacag gcacaactga agacatgaaa tcttctgcta tctttattgc aggactgttt
     4201 tgcagataaa ggttgatgct gttattttgc tttacccatt ctgaaatacc ccacaactcc
     4261 ttttccgatt gcagaacaat cgtatcaaac ccaagcagga aaacggaagt atccgttata
     4321 ccagcacgtc gaactgctcc ttgaaataat tttgtaaata atgtttcact gatagaagca
     4381 gcaggcaatg taagctgaat ccttgtgaaa taaatacatg cggcataaaa tattgtctga
     4441 ataccctcca taatttgatg ggcattcata gtatgcaggg gcttttcttc ccattgttta
     4501 atagcatcct catatccttt tcgtgccgtt tcccagcgtg caacgctgtt cataagtgtc
     4561 ctacgtaaag agcgtgggga aagggatttg atagcaatca aaagtggctt tgatttagca
     4621 gaaagataaa catatccatt aataaccgta tatgccccgt tttttggtaa taattttttc
     4681 gcacttttac caaacatatc tatccataaa agctctgatg cacggtttac gatttcaaga
     4741 ccaagcgtag cgaacaaagg tgtaacagga tttggcaagt gttctgccaa acttcccttt
     4801 gtgtatacaa catcctgttc tggcaatacc cattctggtg gcagaacagt aattggacga
     4861 gcctgaacaa tgtacaattt atctttttcc agtgcccact ctacatccat aggcatttga
     4921 taatacttct caattttctt tccaagctgt atcagttgca ttacctgatt acgagtaaga
     4981 gcatattttt ttctcaacct ttcaggaacc atggtttctt ctgttccttc tgaagtacga
     5041 accgtcataa tctctttatt tgccacttca tacgatataa ttcgttcgga atccttatcc
     5101 accacgattg tatcgggtgt aactaagcta gaaaccaccg cttcaccaag cccccatgca
     5161 gcgttaataa tcatttcgct acgtctacca ttgattgggt ttaacgtaaa cataatacca
     5221 gatgaatcgg aaaatgcaag cttttgtact acaacagcaa gtgcgacaat ctcgtgcttt
     5281 atattattct tcacacgata agcaatagca cgagctgtcc ataaggaagc ccagcatctc
     5341 tttacagcgt caataacttt atcttcacct tgtatgttaa gataagtttc ctgctgacct
     5401 gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg aacggacagc caccgatatg
     5461 ttccctaatc cggcataagc cgttttaatt gcatccgaca cttcctgtgg catttctcca
     5521 ttatggaaaa gctcccctat tttcttagat acatcttcaa gttggctggt attattggaa
     5581 tcaataccat ctagcagttt attaattcga gactgaatat ggttcgtctc aacaaaaatc
     5641 tggtatgaag cagtcgtaac atgaaaaccg tctggcacag ggatacctgc tgtcaatagt
     5701 tttgataaag acatcccttt tccaccaaca atttcaatgg ttgcttgttt atgttccagt
     5761 ggcaagacat aataaatcat aatcattcac ctccatcatt cataattccg tgcagaaata
     5821 tattcattgt ttcatccaac attttttccg aactatccga tggtcgtgac gtgtagctga
     5881 ttgataacat ggagttaatt aacaaacgtg tcacaagagc ggtatccgtt ttgcgaaaga
     5941 tgcctgtggt cacaccatta tcaattaccg attttataat atcttgatat gcataacgag
     6001 catctttaag tctctttcga tactcttctt ctaaataatc agattctgta tttaaccaca
     6061 tcagtacagt tctatactgc tctggaaatc taagatgatt aagcatgatt tttctaaggc
     6121 actgttgtgg tgaaggttca ccatcaataa tcctatgaac ttgttggata gttttttcta
     6181 tttctttctc aactgcatat acaacaatct cgtctttcgt tttgaaaaaa tcgtatagac
     6241 ttgactttcc catgttggct aaaactgcaa tttctctcat tgatgttttt tggaatccat
     6301 tttcagccat gagactcaaa gcaacagcaa ctattttttc tcggcgttct gcttgttgtt
     6361 cgggtgtaag cacaattcct tttggcatag ggcgatacct ccttttagcg accactggtc
     6421 gtttcttttt tatttatcat acagcgacca acggtcactt gtcaaggtta atttttttca
     6481 ttgtatatgg tactcgagat aaaaatgttc attttagaat ttgctctatt taagtagaaa
     6541 taggcttcat cattagaaaa caagactata aatttgagat atttatcatg ttcaaatttc
     6601 atcaatatca aagtttaaga tattaataaa aaaagaatta ctaagaaata acatataaag
     6661 attcaaaaaa ggagttgaac ttaaaattta attacaaaaa atcaaattta agacaactcc
     6721 tttattcttt atctctacta ttctttatct ctatttattt attacaactc cctaacattt
     6781 accactactg ccttaggtct agtcatagcc tcgattgaat acttaacacc ttgagttccc
     6841 ataccagatg cctttacacc taaaaatggg aagtggtctg gacctctttc agtcttatta
     6901 tttatttgaa cagtaccaac ttcaagttta tcagctatat aaaatgcctt atcaatatca
     6961 cttgtaaata ctgatgattg aagcccatac tctgattgat tagcaatttc aattgcttga
     7021 tttatatctt taactcttat aattggaagt actggaccaa atggttcttc ccaagcaatt
     7081 ttcatatcaa catttacatt atctaacaat gttggataga ataaattatt ttttctaaca
     7141 ttgcctgtta ttaactttgc tcctttatgt aatgcatcat caaccaaccc ttgaacaaaa
     7201 tcagttgctt tattatctat aagaggagtg ataactactt catccattgg atttccaatt
     7261 gtcaacttct ctaccaatgg ctttattttt ccgaccaatt tatccgccac actctcttgt
     7321 actaaaattc tctttactgc cgtacatctt tgtcctgaat atgaatacgc tccagatact
     7381 atattcttag ctgcaaaatc taagtctgca tcttctaata ctatagccgc atcttttcca
     7441 ccaagctcca ataataatgg taccattcca gatattttag atatatgctg accaacttca
     7501 gtactaccag taaagttaat aaaattaatt cctttatgcg ttacaacata atctcctatc
     7561 tcactacccc tacctgtaat agtattaagt actccatctg gtatcccagc ttttctcata
     7621 atttctacca catgaagtgc gctaatagcc ccttgtgtag caggttttaa tacaactgca
     7681 tttccaccaa taagtgctgg agcaatcttt gacattgata aatttacagg ataattaaaa
     7741 ggagatatag ctaaaacagt acctaatgga acccttgaaa cataagacat cttatttctt
     7801 gttccaccag ggaaattatc tccactaatg gcttcaccct ccatattttt tcccacatca
     7861 gctgtgtatc ttaaaaaatc tgctgttctc tcaacttctg cccttgcaga ttttatgtct
     7921 ttggctattt caagtaccag tacattagct atctcatcta tattttctaa caataaatca
     7981 gcagccttat gaaatatatt ggctctcttg tatacaggca tttcagccca tacttttata
     8041 ctctcttttg tgttttttat aacctcatct acctcatgtt ttgtcataga ttgtactttc
     8101 ccaacaagag aattatctat tggtgagtaa atttctattg gctttctcga attagattct
     8161 acccattttc catttattaa atttttatat acattttctt ttgttttaag ttcattaaac
     8221 atatttttat tcctctctct gatataactt tgtgtattta aattatagcc atttattcgt
     8281 tatgtaaaca tttctttttt tcataaacgt ttacctataa aaaaaatttt aagttaacgt
     8341 ttttctacat tcttctattt taataacata ctcgctccaa atctatatag gtcacacttt
     8401 aatatcaata tactaatata ccaatatacc aatataccaa tataccaata taccaatata
     8461 aatattataa acataaatca cttgacaaat ttataagtag acaatattat atatctatga
     8521 ctcaaaatat ttttaaagtc atatatatca tatatagata gaaaggattg ataaacatgg
     8581 ctaaaagttt tacagaaata gaaaaagaca atataagaaa aagactaatt tcagaatgtg
     8641 aactcaactg gtctaaatat ggatacaaaa aaactaatat tgatgaactt tgttcaaaat
     8701 caggaattgc aaagggttct ttctatacat tttttgattc aaaagaaaag ttattttttg
     8761 aagtattaaa tggaatccaa aacagactta tatcatcagt agatgaaatt ttaagtaaca
     8821 tgcctccaaa agaaggcttt gcaaaatcct taaaaatgtt atatcgatta tatgataaaa
     8881 atccattttt atattcacca aataatcctg atttcatcag cctaataaat aaactacctc
     8941 aagatatgtt tttacaactt gagtccaata acatacaaag tttttacgac ttaattgaga
     9001 gacacaatct caagttgaag atagaaaaag aaaaagtttt tggtgtttgt aatgctctac
     9061 tatcaagtct attggtaaaa gaaaatattg aatataacta tattgaagtg tttgacttta
     9121 tggtggataa tcttgtagaa cacatacttg aataacaatt taaaataatt tttctaagta
     9181 tttttttata ctaaatagga ttaatatgat tgaattaata caaaaaataa acaatataat
     9241 ttataaaata aatgataaac taaatatcac acaaaactta tagaccctag agcaaaccca
     9301 ttccactcta ctctaagcca tctataatag tttgtatatt tctaataata ttagatatta
     9361 aagtaagaaa aaaattcctt ataataattg ctattatatc aattattata aatattttaa
     9421 taatcttaga taggtaggta catatatggt aaaatcctcg aacactttta ataaaaactt
     9481 caacttgatg atattaggtc aaataatctc attgtttggt gcatcaatac ttaaatttgc
     9541 tttatcttta tacatacttg atataactgg taaagcagaa atatttgcta ctatactagc
     9601 agtatcttca atcccaatta tagtattctc ccctattggt ggagctattg cggatatatt
     9661 caacagaaga aacttaatgg ttatttttga tttttcaagt agtcttacag tactaatctt
     9721 agcagtattc ttattcaaca ataatggttc aatattactt gtaggaatta ttatgaccat
     9781 attatcagtc ataagcacaa tgtatcaacc aactgttcag tcaagtacac cccttttagt
     9841 tgataatgag catttaatga atgcaaatgg tattgtagct ggagttgcat ctcttacaaa
     9901 tattgcagga cctgtattag gaggtgtctt gtatggtgtg attggaatta atgcaattat
     9961 agttatcagt tgtgtaagtt tctttctatc tgcaattatg gagatattta tacagattcc
    10021 ttttatcaaa caagagcaaa acagcaacat tataaaaact atatttactg atataaagga
    10081 tggtattgtt catatatgta aagaaaatag atttatacta aaaatacttt ttttagctat
    10141 agcaataaac ttatttttaa gctctatgtt aatagttggt atcccctata tagtaaaaat
    10201 cactatggga gctagtaact tcttatatgg tctagcagaa ggagcaattt cttttagttc
    10261 actggtagct gctgttagta ttggtatttt ctcaaaacat ctaaaaatca ctaatttgca
    10321 tatttccttt attatatgtg cagttatgtt cataccaatg gcggtttcag tatatccatt
    10381 tatattaaac acaggatttc cagttccatt tttaatcttt atagtatcat ctatgctaat
    10441 attttttaca acgactctta tctcaatata tgttataaca gtcacacaat taaaaactcc
    10501 aaatgaactc ttaggaaaag taatgtcaat tctttttgca ggaacagcaa ttgcaatgcc
    10561 ccttggtcag gttatgtatg gtaaactttt tgatttatta tcagatagag tgtacatcat
    10621 tatactagta gttggattta ttacattact tatagcattt gtatcaaaga taacattgag
    10681 gaacgaaaat ctttagatac aaagaattta gatataaaga tttaattttc actaatttct
    10741 gaatttaaat gcaaaattgt gtatcagtat gttgacgttt tagaaattga ttgatataat
    10801 caatggaaaa taaactttat aaaaattcaa aatattcagc tagaggagtg ataaaaatga
    10861 gttacatgcc aactttagaa caagcatggg aaatattaag aaaatacaat aaggaagaat
    10921 ttcatataag acatgctcaa attgtatctg gagttatgag atactatgca aaggagtacg
    10981 accctgaaag agtagaattt tgggaaatag ttggactttt acatgattta gattttgaaa
    11041 tgtatcctga tgaacattgc gttaaacaaa gagaattaat gacagagctt gatttagata
    11101 aatcaataat cgattctaca ataagtcatg gatatggatt aactggttca gatgttaaac
    11161 cagagcaatt tatggagaaa gtattatttg ctgtggatga acttactggt ttaattggtg
    11221 ctgctgcaat aatgcgtcct tcaaagagcg tttctgattt agaattaaaa tctgttaaga
    11281 agaaatttaa agataaatca tttgctgctg gctgttctcg agacgttata agaagtggtg
    11341 cagaaatgct tggatgggaa cttgataagc ttatccaaac aactatagaa gctatgagaa
    11401 gcttgattcc agagatggca atatagctcc tccatacata taccatagaa gttacatctt
    11461 taaagagcat ttctcttttt gatgcaactt ctttttatat tttgtagaat aaaaagttta
    11521 atattttaaa ctcactatat aataaataat ataatgtata atagtcataa actttctaac
    11581 cttaaaagtt tctaataatc aataaacaat tctaatatac aaatgtcatt atatgattta
    11641 attatatatc ttcaatttat atattaaaat aaatatatat atattaaatt cttatacacc
    11701 aaatttttac attaaacttc tatgcaccac atttatacat attaaattct tatatattaa
    11761 atttctatgc atcactttta tacatactaa attcctatat attaaacttt tatgcatcac
    11821 ttttatacat attaaatcct atatattaaa cttttatgca tcacttttat acatattaaa
    11881 ttcttatata ttaaacttct atacatcact tttatacatt aattcttata tattaaactt
    11941 ctatacatca cttttataca ttaattctta tatattaaac ttctatgcat cacttttata
    12001 cattaattct tatatattaa atttctacac atcaaattta tatacattta aatcacatga
    12061 ttaactgctt tcttcacatt tctaagtgga atagcaattt taactacaaa ttttttatca
    12121 tcatattcaa ttacaacatt accaccatac ttatttacag agttttttac actttttatt
    12181 ccaagtccat gaagagaacc ttttttgtct gtcaaaatat ctccattttt agtaataatc
    12241 ttattcatct tagtattttc aactcttatt aaataaaaat tttgcataac atcaccttta
    12301 acacaaataa ctctatttcc actatcaatt ttattacatg cctcaattgc attgtctaaa
    12361 atatttgaaa atatatgaca tatatccact aattcaacag cttcacaaca actaaaattt
    12421 attcctattt ttagttctat atcattctta tcacaaacct ctttcttttc acttaatata
    12481 gcatctaata taaaattacc agtattaaat aacaagtcat tactctgaat ttctacctgc
    12541 aaattattta aataagaagt aacatcaata ccttttttaa tcatagcatt tatacacaat
    12601 atgtgatttt tcatatcatg acttagtaat ctagttttta aatatttctt ttctatatct
    12661 tcataatact cattttcata cttcaaagta tttttaatca tattgttttc tacaactaaa
    12721 gtattatatt gctgtaattt tttagcaatt aaaactaaag tgatacttga tataagcaat
    12781 acaacagaaa tcataacgaa ttggaaatta tgtattaagc taccagaccc tggattaaat
    12841 atgtattcaa aaattacaaa aaaagatgct atattagcga ctataggaat aactatataa
    12901 aataatgtcc ttttatttat ttctatatta agttttatct ttaaggctct atataaaagc
    12961 aaagacacaa tcaaaagtat ctttcccaat attatagact caattctgta tatatctccc
    13021 gtccccatac tactcatgtc atttatagaa ttaagccaaa ctgttaaaga tatactcaaa
    13081 gcatctgtgg cgattagtaa catccaataa attaaggata ttaatacaga ctttataaac
    13141 cccacatcat atgagatttt ataaaatgca atagtaatta tcatagatat aattactctt
    13201 atatttggag gtacatcaaa aaaatatatt atgtcagcaa aaacaataat acctaccata
    13261 caaaagttta tttttttgtt agaaatcctt ttctcacttg tataatctag cattttctta
    13321 caaagaaacc attctatcat aaaggatatc gcaatgaaaa tattccaaaa taagcttgac
    13381 tttatcataa tactttctcc taacgtatta ctaaattatt tttttatatt ttatgtataa
    13441 tttaattata tacgactcta cacgtgtagt aaatagattg tcaaaattat tccaaataag
    13501 tccatatttc ttttcttttt taattatatc actaaatttg ctaaattgaa ttaaactttt
    13561 tattgctttt tttaataata actatataaa ttta
//
LOCUS       DAGSHT010000091        13569 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGSHT010000091 DAGSHT010000000
VERSION     DAGSHT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.093"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(503..1255)
                     /locus_tag="MJB72_002236"
     CDS             complement(503..1255)
                     /locus_tag="MJB72_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBY8263340.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1314..2969)
                     /locus_tag="MJB72_002237"
     CDS             complement(1314..2969)
                     /locus_tag="MJB72_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8263341.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKIQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(4242..5321)
                     /locus_tag="MJB72_002238"
     CDS             complement(4242..5321)
                     /locus_tag="MJB72_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8263342.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPATVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(5405..6061)
                     /locus_tag="MJB72_002239"
     CDS             complement(5405..6061)
                     /locus_tag="MJB72_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263343.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            6383..6589
                     /locus_tag="MJB72_002240"
     CDS             6383..6589
                     /locus_tag="MJB72_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263344.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(6742..7170)
                     /locus_tag="MJB72_002241"
     CDS             complement(6742..7170)
                     /locus_tag="MJB72_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY8263345.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(7646..8584)
                     /locus_tag="MJB72_002242"
     CDS             complement(7646..8584)
                     /locus_tag="MJB72_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBY8263346.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(9098..9910)
                     /locus_tag="MJB72_002243"
     CDS             complement(9098..9910)
                     /locus_tag="MJB72_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8263347.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(9936..10565)
                     /locus_tag="MJB72_002244"
     CDS             complement(9936..10565)
                     /locus_tag="MJB72_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263348.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            complement(10909..12564)
                     /gene="ilvD"
                     /locus_tag="MJB72_002245"
     CDS             complement(10909..12564)
                     /gene="ilvD"
                     /locus_tag="MJB72_002245"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBY8263349.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKDGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(12888..13187)
                     /locus_tag="MJB72_002246"
     CDS             complement(12888..13187)
                     /locus_tag="MJB72_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263350.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT         4401 a         2437 c         1478 g         5253 t
ORIGIN      
        1 agatttgaat atgaatttaa agttatgtaa aattaaaaat acaggaatga aaattccatt
       61 cctgtataaa atctaacttt tgagtctcag tacaatctta ttttgagaca atcttttttg
      121 ttttatattt tcttatattt aaaatataac gcatatctta tgcaacatat tcatattttt
      181 ctttatattc tttcatcgca tcattccaag cgtcttgcac tgttacacct cttttattag
      241 caattttttg tgccagttca attatcttca tagctcttct tgatatgttc ataaacattc
      301 ctcctacaaa ttaccatata tttcatatat ataaatcaag gttatatttt tatgatatat
      361 aatccttcta ttgtaagatt acagtttata tgtgtattat atatgaacta tattagaaga
      421 taattagaat ttttataaag ctatatgatt agattaaata taaaagcttt acaattactt
      481 ataaatttaa aaagtctata aactaaacta atgtttgata taccaatttt acgaccataa
      541 ataatgccat tactaaaaat attggtttta tcatctttga acctttcttt atagcaactt
      601 tagcacctac ataagctccc attatcatca ctaatgcaaa cactatagca attttataat
      661 ctacttgacc atttatcatg aataataaca atgaagttat attacttgtt aagtttaata
      721 tttttgtatt tgcagaagca tgtaaaaaat catatccata tatttttata aatccaaagg
      781 tcaaaaaagt tcctgtacct ggaccaaaaa aaccatcata aaaacccatt ataagagcca
      841 taagcatccc tagtcttaaa tttttcttat taacagcttc aaaattatct tcttgaccta
      901 aatttttaga aataaaggtg taaatagcca ctattaaaat cataaccaca accaaaacat
      961 ttaatatctc ttcacttatt gataatacac atctcacacc tagaacacta ccaataatag
     1021 taaatggtac taattttttt aaaaggtcgt tattaatttt tccagacttt gcatatctat
     1081 aggcactact tatacatcct gctgatgctg caaatttatt tgtaccaatg gcaagatgta
     1141 ctggcactcc aattgccata agaacaggca tgcttataag tcctcctcct cctgctatag
     1201 aatctacaaa agcagctaaa aaccccccta tacataaaaa aataactgaa aacataaaat
     1261 tcctccatca ttctctttca atttttctta caatatatat tatataatct tatttaatac
     1321 ttattatcca gcatattctt tctatattca tttggagtta tactattaaa cttcttaaat
     1381 atttgtgaaa aataactact gttattaaaa cctactttct tacaaatatc actaatactc
     1441 atgcttctat ctaacagata ttttttagat tcttctatcc taagaagatt tatatactca
     1501 gttattgaaa ctttatttct ttcattaaaa atagaactca aataatttgg acttataaaa
     1561 aaaacatctg atatactatt caaatttata ttttctttat aatatttttt tatatattcg
     1621 tttatatttt caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata
     1681 cactctttgg aaaattcaaa taatacattc cataaatctt catcactgtt tacaagtgat
     1741 atttttttat tatatttatt tcttaaataa gatataagtt ttaaatcctc taaatctcta
     1801 agcactgttc tagacaatac aaatatcatt tcctgaatag tatctctaac atcattatat
     1861 cttaatttgt ttgtatctat tagattataa acaccttcta aattcatatt tttaatttct
     1921 ttttcaatat aaagttctaa ttcaaaggga tattcttttg aagaattatt gaacatatct
     1981 atcggattaa taatatactc ttcttctaat aatctactat ttgctttttt aagtgcctct
     2041 tcaagtctta tattttcttt tctactcttc aatatctcct catcttttat actaagttga
     2101 ttttcaaaaa aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt
     2161 tcttttaata catataacct acttttagga tttaatggaa attcattgta aattttgttt
     2221 atgtatttta tatcaatacc agaactactt gataattcag ataaattttt atctatatca
     2281 atagacttat atactataaa attaccacat tctatatatc caactatatt ttcttctaat
     2341 actataggta tctctaaact ggaaacacca tactcacaat ggtacacttt ttcaaagtta
     2401 tcattattta tgcttttaac aagtttttta gtacactgtt ttatattttt acaaatatcg
     2461 caaaatttat tttctttctt agtctcaaat atttctttat actttaagtc atatacagat
     2521 attctaagct tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta
     2581 actaactcct gtatattgtt ttcagaaaat actataggta tttgtctcaa actcgaaaat
     2641 ttatgaggaa cataagtaat taatattact aaatcaattt taccagtgtt tataatttca
     2701 tgttctgaat atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca
     2761 tcaattattt gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga
     2821 aaatgctttt cttggcagtt tcccggaaaa aatcttacaa ttccagcact aagtctattg
     2881 aattgtattt ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact
     2941 tcattatttg actcactaaa atactccata agctcacctc ttagtttttg tctaaatatt
     3001 ttttcttccc ttcttatatt aaaataatat catcatcatt aactttcatt actataaaat
     3061 catcatactc ttttaatact cctgtaattt tagttgcact tagtttacta caattcagtt
     3121 ttttaatata ttgataaatt ctataattac ttttttattt tgtaaaatca gtgttttccc
     3181 aaaataatta tatctataaa tgaaagtatt tttactgctt acatcaatat atgttatcat
     3241 aattttcctt tttattgcat atataaataa ctgtatataa cattgtccaa tactatatag
     3301 aaatacatct aagaaagaat attattaaat tgttccaatt agtattattt ttcttataga
     3361 aataaaaaaa ctgttggatt cttttagaaa aattattttt tacatacctt ttttgcttag
     3421 atagtctatc ttctcagcta ttttgtatat acactcttct agttcatgat tttattttaa
     3481 tatcttgctt atattgctga aataattgaa ttcttttatc tatcatattg tctcttatat
     3541 cataaacata ttgtttctta tatcacaaaa atgaaagcat tcttatatct tcaatatata
     3601 aacctgcttt ttttaatctt gtaataaata taggatactc tatattctct tcctagaaat
     3661 aactaatacc acttattttt ctattatata gaaagtaata aatctttttc ttaataaaat
     3721 tcaatagtaa gtggaataaa attttatatg aataagaaca ttataaaaat actttataca
     3781 atagtcaata aaagatatta gcttcaaatt atgttaaagt tgtattattt aaagtgaaaa
     3841 aacaaataaa aaagaaataa tagtataagt agaaaaatat acttaaaaca aggcgaagcc
     3901 tctcttgtat ctaaatcaag tagagaatta aatctaaaaa agaaaataaa tataaaagat
     3961 gactcctgta cattacaaga atcatcttat ggtttcataa tcactccatg gtttttaact
     4021 gtctgatata aaggtctcag ttcactcata gcatgaccac cttttccgat tgatgacccc
     4081 gtcacttatg gacaaaatgt ccataagtga tagtcctctc ttaaaaccct ctaaatagat
     4141 atatctcaag caccgcattg aaaccagttg ctggaaacaa actgaagcgt tctggaatag
     4201 taaaagaaat tgacaccata tccacgttac tgtcctagtt gttactttgc taacttcgga
     4261 gcgatttcct ctaacaatac gcagtcaaat tctgcctttt tgctaagaaa tggcagatgg
     4321 tctgcatctg gtataaaaaa aatctgtttg tctggcgcca ctatttcatc aaaaaatttc
     4381 ttcgctggaa gataggggct attatagtca ttttctccca tgatgtagta tacagggacc
     4441 tgatagtcaa ctccataatt tcttatgtca gcttcttcca ctaaaaaccg caatacatca
     4501 ccttgattgt caatcgcatt tggattaaca atggccgtat tttttaactc tctcaatgaa
     4561 taatacgggg ttgccagtat actgattata ttgcccaaat ccatatttat agaaagccca
     4621 tacttgcttt ggtatgttct tactgtcata agagctgcca aatagcttgg accatatttt
     4681 gctggcggat atggtgcaat agcttccagc gccttgatat ctttttgctt ccctgcagct
     4741 ttcaccattt caagtgtctt ttcatagccc acgcgctcat tgtccaccat attaacggtc
     4801 tgcgcaatcc caatgtacgc gctgtaatcc tccggatagg tttgcacagc catagttccc
     4861 agcactgttc cccagctatg acctataagg ataattttct ccttgttgaa ctcttgcttc
     4921 aaatatgctg tcacctcatg gacgtcttgt agaacacgct ccgcgctcat ggtctgcaaa
     4981 actgttgccg gatcgttcgc aaagtatgtt ttgcctacgt ttcgctgatc ccagttcacc
     5041 acagtaaaat cattttccca ctcatactgg tacagatgta aaaatggcat cattgaatta
     5101 ccaggacccc catgaaccca aaggatgatg gggttgtctg cgttttgtcc ccggatatat
     5161 aacgcctgct gtatgccgcc gatctccacc atttccatgc ggtcgatgcc aggagcttct
     5221 attttatggc ttgtacgtat aatcgtgggg gctatcagac gcaaaccggc aaacgccaga
     5281 acaaaaacag caactagtat gcaaagtgtg attaatatca ttttaagtac cttctttcat
     5341 gaattttttt agccatagat atctcctcta acattaaaaa aagtatgtta ctattttagt
     5401 tttgttactc tacctgtccc attggtttcc tttcataaaa aatattaatt gattctataa
     5461 tctcatcttc cagaatatca actcctattt gtctcataag aaactgtaag aattgaaacc
     5521 gtttacgaag tgtaggaaag tcgccctgaa atacatctgt atcacaccaa aagttaaaga
     5581 gcagcaaaat tacctctgcg cattcatctg ggaactctgt gatgatgctt ctatcctcta
     5641 tgccttctcg taaaacattt gctaatattg gtgctagttt tttcaaatta tcctgtgtaa
     5701 atgccagtat gatatgcgga ctttctatcg cgcttgtaat catgttacta gattctttta
     5761 tcacctcatc cgttaaatgt cgcctaacta aacttctaag cttctctttt gctgtaagtc
     5821 catgcatttc accgagccat tgatttactg tttctattat ctgttcaaag cgcctttcca
     5881 tgaccacatt aaaaatatct tcttttgaac tgaagtgatg aaaaattccg ccctttgaca
     5941 ttcccgaagc atcaacaata ttttgcatac ttgttttgtc atatcctttt tcagcaaaca
     6001 actttgcaga aatggtaatg atattttcaa tagcttgctt tggattgttc tttctagtca
     6061 ttatataact ccttcttatt aaaaaccgac tgttggtttt tattatataa gctattcctg
     6121 cttctgtcaa gtaacattgt gtttatggca aaacaataag gtttggtggt tagacaggta
     6181 ttaattagac taatgcgact ttcccctaga tggaaccctt ggtaaatttg caatctttac
     6241 cggctttgat tatatctgta agcaaggcag gtatggccac acctgccctg tttgcccctc
     6301 ggatattcat tcctatccct cctctcctta gccattacac caaaacgccg ctcattgtct
     6361 ccaatggcgg cgttttaaag atatgatttc tcatgctgct gaaatatgca aaaaatataa
     6421 gatgcccttt attatttttc ctacaataga aaagaaaagt attgaagaat gggttaatat
     6481 tggagcaaat atgctttgtt tggcacaaga tactaccttt atcacttatg ttatcaatca
     6541 attattatct ttttataatg agattgtacc aattcaaaaa caacattaaa ttataaatct
     6601 aattttatat taaaagagac taatatttta tctatctcta ttaaggatat tttcttatgt
     6661 atcataatat tagtctctct tattattata tactgctatc atcattagtt gctatttata
     6721 ctatttttat aatatacaaa tttattcagc taacgttttg cctaattcta tacactcatc
     6781 ttttgcttct tcatttgggt cttccattgc tgtaattcct tctccaccaa taattgtagc
     6841 accagcattt tgcatacgtt cttcccagtc tctcatccat tcacaatttc cccatccata
     6901 tgaaccaaat aatccaatct gtttacctga taccatagat tctaattctg aaacaaatgg
     6961 ctccatatac ccctcttcta gttgctctgc tcccatagat gggcaaccta atgcaaattt
     7021 atcttcttct tttaaatcac ttggtttcag taagctcaca tctaaaactt ctggtgtctt
     7081 accttttaac ttcacacctt cagccacaaa atttgccatt ttttcagtat ttcctgttcc
     7141 actccaataa acaatatata ttttactcat ttttacctcc tgtagtattt tttaattaat
     7201 caatagttag ttatagctaa ctaacatcta aaaaacaaaa gatttcttta tctgaaatct
     7261 ctttattttt ctaaatatag aatatcatgg ttgattttga aattcaatac ttattgataa
     7321 tatttatcat ttatatttta tattcatttt ataatatcaa aacactgtat cgtatttcta
     7381 atctttcata gaaaaatttc taacttatag tacttttgca tctcttataa atcttttata
     7441 tgtataagta catagaaaaa cacacctttt ccagatgtgc tatctataat atcgtatatt
     7501 tatttttcaa ttctaactag ctaatatgtt tataaatttt atcacttaat acctttatac
     7561 attgatgtaa aatcttacac ctaagtaatt tttattattt gtctcgactc tatataatat
     7621 agctatttta tcatcaaatc aaaaattatc ttttgctatt attcttgtat ttataaagat
     7681 ttaaatataa gtatccattt ccttgttccg ttctaggtat tccaagcgct ctcgtacatt
     7741 ttattaattg tgcatacagt tcagcttcat ctaaatctct ttcaaactcc tctcttgccc
     7801 attctttaat tagtgctaat gagcctgata cgtatggtgc actcatactg gtaccactca
     7861 taatagcaag tttattatcc atataagtcg atataatatt tcttcctgga gctaccaaat
     7921 ctattgtagt atttgaatta ctaaactttt caaccaaata gttctcattt attgcaccta
     7981 cttctattac ctcagcataa ctggctggat aactatactc acttgtacta gaatcaccat
     8041 ctccattatt acctgctgca cacactacag aaatattatt ttttactgct tgcatgacag
     8101 cattttttaa attcttatca tctttgtttc ccccaagaga catagatata atatcaacct
     8161 tgttatttac agcaaagtta atagcattaa ttatactttg atatgttcca gtaccatctt
     8221 tatttaatgc ttttgctatt aataatttac aatctggagc tactcccata acttcattat
     8281 tataattaga tgcagctata atacccgcca catgagttcc atgaccatta aaatcctcat
     8341 atatattttt atttccatta ctgtcatcac taaaatttgc accaccaatt atttttcctt
     8401 ttaaaagagg atgagatata tcacaacctg tatctattat accaactaca atatttttac
     8461 cagtataacc ttcatcccac attcccctag cattcatttg ttttattcca tatgggaatt
     8521 tattttttgc acctcttaga ttttcattta tttcatatgg aattagtttc attcttttat
     8581 tcatcatata tcctcctaaa aattttattg cttataaaaa tcatgaattt tattttataa
     8641 taggtttata aatcaaatta aaacctatat ctacttattt atatttatga tatatttttt
     8701 atttttagga gtcatatttt attgtatata aaataatatt tagataacta tattaaaaaa
     8761 tacatgtttg cttatataca aaacttacta aaatgtcttt aatatttttg tatacaaaca
     8821 ttatattttt tatataaaaa gctcataaaa attttgatta tattttcata agttaacaaa
     8881 tgtgggtata atatgaattt acaaaattcc tctgtttgta taagtgcttt accaatacaa
     8941 caatatataa tcacattgaa gctatatttg tatttatagt agataatgta tcaaaaattg
     9001 gttatgaata gtaaaaatac cagttaagca tattttcatg tttctatttt taacattaaa
     9061 tttccaatct tcatatgtat atttttaaca aaggtgttta cttatttaaa aactctaaaa
     9121 ctctttcatt aaaatcctgt gcttcttcat atgtagcgtg tcccaatccc tcatagataa
     9181 atatttcgct ttttttaatt ttttctacta aatgaaaaga agcattattt ccaacaattt
     9241 tatcattagc acctcctata attaaggttg gacatgttat tttatttaat tcggaaaagg
     9301 cattatgttc tatgcaagag gtcgcttgaa taataaatcg tttaaaatcc tttggtttac
     9361 ctacttttcc taaaaaagga ataaataatt ggtatttttt taaatatctt tcagaatagg
     9421 atttctttgc tgtgtcaatc attaaatcat tgtaattctg cttcttcgcc atatcaatcc
     9481 aactacaaat aacattttgg atagtatcat tttgctttga agaagtaact gccaacacaa
     9541 gtttctcaac taactcagga taatcaattg ccagatattg agctatcatt ccaccttgag
     9601 aaacacccat tacttctgct ttcataattc caagtttttt cattatatct gcttggtctt
     9661 tagccatatc ccttgtggaa tatttttctg taatttgatt ttttctacta aatacataaa
     9721 ccttataatc ttttgtaaat tgcttatatc taaaagcaaa agcaatcgcc tgtatttttc
     9781 catgtaaagg aaataaacca tctcctaatc ctggtaaaat aattagtgtc ttcttaccac
     9841 taccaaattc aatataattc actgtattac ttcctatcat catttgacaa ctttttgcat
     9901 tataaaacat agtctattcc tcacttttcc tattatcatt gtttaaataa atctttaaat
     9961 ttttgaatac catactcatc aattagagga atatccattt tttttaacat gaataataaa
    10021 aaatccaacc tattacatac ttcactgtat tcacgtttaa ataatacagg gtcaagtaaa
    10081 atatcaatac taaaaacgag catttcagct aattcctctg gatattcaca agaaaattct
    10141 ttattttcaa caccttgttt aattatctta gcaatattgg gtgcaactac attgttgaga
    10201 ttacgaactt cctctattaa agcgaatggg attttttcta accagctaca atgtaccatt
    10261 tttctacgtg tgtaatcatt cgcactatga acaaccaatt ctattatttt ttcatttgct
    10321 gtaagggtgt catcagcaac aagatgctct aaaaaattat tcatttgtga gtaaagttct
    10381 tttgtgacac ttctcagtat atcttcttta gttttaaagt gatgatacat tgcacctcta
    10441 gatagaccag ataaattgat aatatcttgt gtagatgttg aatcatatcc tttagacgaa
    10501 aatagtgaaa atgctgattg gataattttt tctttactat ttttactttt ttcttctcta
    10561 ttcatatttc cctcctaaaa acagactgtc tgtttgtttt ttattataca ttttaatatt
    10621 tattatgtca agaattaact tactttcaat ttcttcatag ttcagaagga acgaaaagca
    10681 ttccacatca aacttttaaa aagttgtatt acctcaacaa ggtctaaaag atacatctct
    10741 atgctgtact cgtattcctt ataatagaga tgtattgttt tcaaagataa tcaagatttt
    10801 gctatgtttt tcatacaaaa taaaaaggat acctcattag tgctataaca cctaagatat
    10861 ccaattaata ttttcttaac tttttctaat ttacataaat atagtctact attttaaaac
    10921 tgctcctgta tttgctgatg ttaccaattt agcatatcta cttaagtatc catgctttat
    10981 tttcggttct aaaggtttaa attttaattt tctattttct atttcaactt catctacttt
    11041 taactctaat tttttgtctg gtatgtttat agaaattata tctccttctt caactaaccc
    11101 gattaaacca ccttccatag cttctggaga aatatggcct atagatgctc ctctagttgc
    11161 ccctgaaaaa cgaccatcag taagaagtgc tacatgctta tcaagtccca ttcctgcaac
    11221 tgctgatgta ggagatagca tttctttcat tcctggacca ccctttggac cttcatatct
    11281 tataactata acatcacctt tgtttatttt tttaccaaaa atagcattta cagcttcttc
    11341 ctctgaatta aaaactcttg ctggtccttc atgaactaac atttcttctg caactgctga
    11401 cttttttaca acagctccat ttagagcaag atttcctttc aatattgcaa gcccaccttg
    11461 gttactatat ggattcttta atgtatgtat tacttcttca ttttctattt cacaatttct
    11521 tatattttcc cctatagtct tgcctgttac ggttttgcaa tctaaattta ttccatttat
    11581 ctttgaaagt tcgttcatta tagctggtat tcctcctgcc atatgtaaat cttcaatatg
    11641 atgttttcca cttggactta attttgttaa acaaggagtt ttttcactta tttcatcaaa
    11701 aaaatctaag ttaagctcta ttcctgattc ataagctatt gcaggtaagt gaagtactgt
    11761 atttgtagag ccagccattg ccatatccac agctatagca tttttaaaag catctattgt
    11821 taaaatatct ctaggtttta tgtcgttctt aagtaactcc ataacataca tacctgcata
    11881 ttttgctatc ctttttcttt ctccagaatg tgacgcagca gtaccattat atggtatacc
    11941 catgcctaaa gcttctgtta aacaattcat gctatttgct gtgaaaagac cagaacatga
    12001 accacatcca ggacaagagt tcatggctaa ctcttctaat tcttcttcac ttactgttcc
    12061 atctttacat gctccaacac cctccattgc agagttaaaa tcatatactt ttccatcttt
    12121 tttacctgga agcataggcc ctccactcac aacaatactt ggtatattaa gtcttgcagc
    12181 agccattaac atacctggca ctatcttgtc acaattaggt ataagtacca aagcatcaaa
    12241 accatgtgcc atagccatag cttctattga atctgctatt aattctcttg aagccaggga
    12301 ataattcatt ccaacatgcc ccatagctat accatcacat acccctatag caggaaattc
    12361 taatggcaaa ccaccactca ttcttactcc attttttgca gcttctgcta tttcatcaag
    12421 atgcagatga ccaggaatca cctcattttg tgcatttact atacctatta atggtctttc
    12481 aatttcttct ttagtaagac ccattccata cattaacgct ctctttggag ctccttctat
    12541 tccttttttt atatcacttc tcataatatt acctctttct tactttttta aattttcata
    12601 atattattat aatatatttt tctattaatt aattacgaaa attgtaaaaa aatattagca
    12661 tatactaatg aaaatttatt acgatttttt atcaatatct tacacaatcg tattatataa
    12721 tattatcttt tcaatatatt ttataaacta atatatttta caaattgttg agtaatattt
    12781 tatgatataa atcagaagag gcacttctat aatagaaagt gctcaactat gaataagatt
    12841 aataatatat ttgccttaga tatatagact tttataaata ttaaagatta atcaaatctg
    12901 atgtttgaaa ctttatccca attaatcttc cttgaatagt cttctggatg tgataatcta
    12961 actatatgag ttgaattatc atagctgtaa taaattgtag tagtaccatc aactggattc
    13021 tttttgtatc cttgatacac agcactattt ggtactttct tttcaacaaa gctatatgca
    13081 gatgaagtat caatagtttc tattttatta ggaaatataa acatataaaa agctactact
    13141 ataaaaatac atatgcatgt aatactatac catttaatcc atttcataaa attcctccta
    13201 ctttaccaaa atatactatt attatttcca tatatttata atatttatac atacgataaa
    13261 atttttatat ccctaactat ataattgtag aaactttgat tatatatatt ttttaatctt
    13321 aataatatat ttttataaat taataaattt ttaagatttt attaaaagat ttttaaaact
    13381 tacagtttaa tataaagata gtacttataa aactaaaaac ataaatttga aaggggacaa
    13441 aataaaatga tatggacatt ttattcattt atttgcgtta tattaccatg cttaatatac
    13501 caaatagtcg ttataaaacg aaaaaatttg aaagttgaaa aaaatgtaat gatacattta
    13561 gtatgggta
//
LOCUS       DAGSHT010000092        13559 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGSHT010000092 DAGSHT010000000
VERSION     DAGSHT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.094"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..440
                     /locus_tag="MJB72_002247"
     CDS             <1..440
                     /locus_tag="MJB72_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263351.1"
                     /translation="YLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQA
                     ISRLNHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQ
                     LTLNNSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            430..750
                     /locus_tag="MJB72_002248"
     CDS             430..750
                     /locus_tag="MJB72_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263352.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            948..1817
                     /gene="galU"
                     /locus_tag="MJB72_002249"
     CDS             948..1817
                     /gene="galU"
                     /locus_tag="MJB72_002249"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBY8263353.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            1841..2854
                     /gene="galE"
                     /locus_tag="MJB72_002250"
     CDS             1841..2854
                     /gene="galE"
                     /locus_tag="MJB72_002250"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBY8263354.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            2966..4900
                     /locus_tag="MJB72_002251"
     CDS             2966..4900
                     /locus_tag="MJB72_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBY8263355.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            5055..5918
                     /locus_tag="MJB72_002252"
     CDS             5055..5918
                     /locus_tag="MJB72_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBY8263356.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(6126..7007)
                     /locus_tag="MJB72_002253"
     CDS             complement(6126..7007)
                     /locus_tag="MJB72_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8263357.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            7420..8268
                     /locus_tag="MJB72_002254"
     CDS             7420..8268
                     /locus_tag="MJB72_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBY8263358.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMLAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            8347..10269
                     /locus_tag="MJB72_002255"
     CDS             8347..10269
                     /locus_tag="MJB72_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY8263359.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            10351..11214
                     /gene="aroE"
                     /locus_tag="MJB72_002256"
     CDS             10351..11214
                     /gene="aroE"
                     /locus_tag="MJB72_002256"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBY8263360.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TIDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKNVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            11414..12631
                     /locus_tag="MJB72_002257"
     CDS             11414..12631
                     /locus_tag="MJB72_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8263361.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            12867..>13559
                     /locus_tag="MJB72_002258"
     CDS             12867..>13559
                     /locus_tag="MJB72_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBY8263362.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQIELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLA"
BASE COUNT         5264 a         1501 c         2516 g         4278 t
ORIGIN      
        1 gttatttaaa tgaagttgaa aaaagtttag acaatatcat ctttgtaatt tcaggagaag
       61 ataagaagaa atacaattat agaatgttaa aagaaagtga aagtgcaagt cttgatgtat
      121 ttttaaaaca agccatatca agattaaacc atttagtaga aaatatgtat ccttattatg
      181 tagatgaaac tagtagggaa atgtttaagt tatttgagtt attgtggaaa aactatagaa
      241 atattgatga tgatagtata aaagaaatat tattcgagag aataacaaat acatatgtat
      301 ttaaacaatt gacattaaat aatagtacaa aagaaagaag aaaagacata attgaaaaaa
      361 tatataactc tgttgattat ggaaccaata aactagatga aaatatgttt ttcacaggag
      421 gaataaagta tgtcaagtaa atacaatgtt tggactttta actatgagtt cttaggattg
      481 gactcagggg atgaaaaaaa taatgaagtt tattacgaga ttgatttaga tgaaatttta
      541 aatgaaaact atatagatga agatatagat agtggtttag attctgatgg aaatagtatt
      601 ttaggagaat taatatcaga aatggatata gatgaggatg tcagtgttga tatgacatat
      661 caagaactag aagttgactt agaagattta gattcttatc tagatgaaga tatagatagt
      721 gatataaaag gcatcttaga tgaaatttag tacctgtata agttttggat aaaatataaa
      781 caatacaata ttaacttata aaactaaagg tagatgaaat ttcatctacc ttatattatc
      841 ttatagcatt aaatattggg ataatataga gtttgaagaa aaagtataaa tatggtatat
      901 taatttggta caatagaaaa tttacgaaga agggtgaaaa aatgcagatg acagttaaaa
      961 aagcaataat accagcagca gggctaggaa ctagattttt accagcaaca aaatcacagc
     1021 ccaaagaaat gcttcctata gtagataaac caactttaca gtatataata gaagaagcta
     1081 tagaatcggg aattgaagaa attcttataa taacaggtag aaataaaaaa tctatagaag
     1141 atcattttga taaatcagtt gaattagagt tagaactaga gcaaaaaggt aagacagaga
     1201 tgttagaaat ggtaagagac atatcaaata tggtaaatat tcattatata agacaaaaag
     1261 agccaaaagg tcttggacat gcaatttact gtgcaaaaag ttttataggt gatgaacctt
     1321 ttgccgtatt attaggtgat gatatagtag attcagatgt tccgtgctta aaacaattaa
     1381 tagatactta taatgagtat aaaacaactg tattaggtgt tcaaaagata gctaaagaag
     1441 atactaataa atatggtata ttggatgtaa aacatatcga agatagagta tataaagtta
     1501 aagatatggt tgaaaaacct gcaattgaag aagctccatc tgatatagct atacttggaa
     1561 gatatataat aactcctgca atatttagca tattagagaa acagacacct ggtaaaggtg
     1621 gagagataca actgacagat gcacttcaaa ctcttggaaa acaagaggct atttatgctt
     1681 ataactttga aggtagaaga tatgatgtgg gagataagat tggtttttta gaggcaacaa
     1741 tagactttgc tctaaagaga gaaaatttaa aagatgattt aatggactat atgagaaaaa
     1801 aagtaagtga aaaataaata ttagaaaaga ggtaattaca atggcagttt tagtagctgg
     1861 aggagcagga tatataggaa gccatacagc aatagaactt ttagaatcag gttatgaagt
     1921 tgttatagtt gataacttaa gtaatagcaa ctcaatagtg gttgatagaa tcaaggaatt
     1981 atctaaaaaa ccagtaaaat tttataatat tgatatcaga aataaagatg aaatgcatgt
     2041 tgtatttaaa gaaaataata ttgaatcaat tatacacttt gcagctttaa aagcagttgg
     2101 tgaatcagta gaaaaaccaa ttgaatatta cagcaataat ctaataagta ccttaaattt
     2161 atttgaatta atgagagaat atggtgttaa aaagtttgta tttagttctt cagctactgt
     2221 atatggagac ccacatactt gtcctatatt agaggatttt ccactttctg taaccaatcc
     2281 atatggaaga actaaactta tgatagagca gatgttagta gatatatcaa aagcagataa
     2341 gtcattggat atagctcttt taagatactt taatccagtc ggtgctcata aaagtggtag
     2401 aataggtgaa gaaccaaatg gagttccgag caatctaatg ccttatataa caaaaatagc
     2461 agtaggtaaa ttgaaggaat tgagtgttta tggtaatgac tatccaacac atgatggaac
     2521 tggagttaga gactatatac atgtattaga tttagctgct ggtcatgtaa aagcattaca
     2581 aaaattagaa gaaaatccag gacttgttgt ttataattta ggaactggaa aggggtatag
     2641 tgttcttgat ttagtaaaag cattttctaa agcaagtgga aaagaaatac catataagat
     2701 tgtaggaaga agggctggag atgtagccat gtgttatgca gattcttcaa aggcagaaaa
     2761 agagcttgga tggaaagcta agtatgaact tgaagagatg tgtgaagatt catggagatg
     2821 gcaaagtatg aatcctaatg ggtatgaaga atagaatata tacttatatg tataaatgta
     2881 acaaatttgt aaatataagg tattggtaat tttatgttat aattagacac atgtagtaat
     2941 gtttctaaaa gagggggaag ttatagtgaa aaaaagtcta aaaaagtatt tagtactagc
     3001 actgacttta gttttagtat ttgcatgtgg cgtaccagaa tcaagcgcag ccagtaaaca
     3061 tgtgattata gtaaattcaa gaaaaaatac tctaggatat tttgtaaata ataaacttgt
     3121 aaaagaattt agagttgcaa caggtaaaaa ggggtcagaa acaccaacag ggaaaacaaa
     3181 agtagttaac aagataaaaa atagaccata ttataaaggt aatatacctg gaggaagtcc
     3241 aagaaatcca ttaggagata gatggatggg attagcatta aaaggaactt atggagatac
     3301 atatggaata catggaaata ataatgagag ttctatagga aagcatattt ctggtggatg
     3361 tataagaatg cacaataaag atgttagatg gttatttgac caagttcctg taggttcaga
     3421 tgtaattata gattatagta atgacagcta tgtaaagata gcggcaaaat ataagataaa
     3481 tctaaatcaa acaggatgga aaacagaaaa tggtaagaag tattatgtaa aatctgatgg
     3541 aacataccaa aagaatagtt ggctaaaagt aaatggaaaa atgtattact ttgatgcttc
     3601 aggagtaatg caaacaggat ggaaaacaat taataacaaa aaatattatt taggaactga
     3661 tggagcaaga gtatctggat ggaaagtaat agacggaaaa acttattact ttaactcaga
     3721 tggagtaatg caaacaggat ggcaagaaaa gaatggaaag aaatactatc taggatctga
     3781 tggtttagca gtaacaggat ggcaagaaat agatggtaat aagtattact ttgacaaaac
     3841 aggaataatg cagacaggat ggcaacaaat agatggtaag tcttactatt tagacaaaga
     3901 tggaaagatg ctaactggtt ctcaaaaaat agatgggaaa gattatactt ttaatgaaga
     3961 tggaactata aatcctacat gggatacaat tataggtgca aatagatttg atacagctaa
     4021 aaagataagt tcagttggaa actggaatgc tgatagctca gatactgtaa tattagtaaa
     4081 tggtaatgca atagctgatg gtataacagc aactccttta gcaagtagct atgactcaac
     4141 aatactactt actaatacag ctaatttacc aactgaaact gttgaagaaa tgaagttatt
     4201 agcacctaaa acagtaatct tgataggtgg agaaaatgca ataagttcta aattagagca
     4261 agaaataaaa actactttca acacagaaac aaagagaata gcaggtcaag atagatatca
     4321 aactgctaca agaatagctg aagaacttgg aaatcgagaa gaaataaaaa ctgcatatat
     4381 ggtttctgga aatggggagg cagatgcttt atctgtagct tctaaagctg gggaagaaaa
     4441 acaacctata atattagtca ataaagatgg aattacagaa gaatcttata aatggctaac
     4501 agaaagaaaa ttagaaaatg catactttat aggtggacca agtgcaatta acgattcagt
     4561 aatagcaaaa atgaatgata taactactga agatatttca ggaaatagaa tatatggaga
     4621 tagtagagta gatactaatg ccaaagtaat agaaaaattc tatggagata ctgatttaca
     4681 ggctgtatta gttagtaaat cagatgcttt agtagatgct ttatcagctg gaccactagc
     4741 tgtaaaatta cattcaccaa tagttttaat ggataattca ggattatcat cagagcaaca
     4801 aagagtattt gcaaataaaa aagttgaaac tccttatcaa ataggaggcg gagtatctta
     4861 tatagtaatg gataaattaa tggatattct tgcaaaatag taaaaatata tattgaaaaa
     4921 taattataat tgagatgtga aattcaatca ataaaagctt gattgtttta catctcaatt
     4981 ttttagtata aaattaagtt taagttatat aaaaatcttg gaatattttt aaaactcata
     5041 taaaatgaat atagatgcta tcatcatatg ttaagagggt gagtaagatt ataaagaaaa
     5101 aatatataat gtttattgat gaaagtggaa gagctgatat aaatcatgaa gacccattta
     5161 cattgacagg agtaattttt gaatataaat atgcagtaag tcaaggcgat tatgtatgtt
     5221 ctttgaggaa agaaatggat gcatttaaag agtattgttt tggaactagt aacataggaa
     5281 tacatttgac agatatatca agaaaatcaa gagcatttga gaagtttgat gattcacaga
     5341 ttaagttatt ctatgatgag ttgcctttat ttctatctag actagagtgt actataatat
     5401 cagtaacagt agataaagct agattaaaga aatattatgc accttcaaag gaaccgtatg
     5461 ttgtggcttt tatacatgtt cttcagaatt tttattcatt tgtaaataat gaaactgtag
     5521 aatctgcacg aatagttata gagtcaaggg atgatgtctc aaacttaaaa gtacaaaagg
     5581 cgttttttga tgtatttaat aatggaacaa tatacttaga tatagatgaa gaattaagag
     5641 ataaagttaa gggttttata attgccaaaa aagaagatgc tgactataga gaaggtttag
     5701 aaatagcaga tattttatgt aatcctctta gcagagcaag aagagggtta atagaagcta
     5761 atccaaagtg tatgcaatat ggagaaaaaa acaaaatatt caaatctgtc agaaacaaaa
     5821 tatatactcc aacgagtgtg aatgatataa gaaattgggg atttaaagaa gttcctattg
     5881 tagagcctgt tggaccatgg cttgaaaaaa tgcgttagat acaattgaca aattttaaaa
     5941 aatctgatat aatgtaagta acctagttat tcaggaactg aatccagttg ctgttgtata
     6001 acgataatta ctgagactcg gatgccgact gtcagttagg aatctatact tttaaaagta
     6061 tagatttttt tattttataa aaagaataaa agaggatatt taaaaataac ccctttatta
     6121 ataaattata attgagcgtt tttaatgaca aagtttttaa agtttttaat tgcaggaggc
     6181 aaatatctat cttttataaa tgctaagtaa aagtttcttt cccaacttgg gtgaataatc
     6241 tgaattactt ttacttttgt aaagctaagc atattcatat ttgggactac agcaattccg
     6301 aaattttttg caactaatcc agcaattact tggtcttcct ctacttcata gactatatta
     6361 ggctgttgat ttattttttt aaacatatca tcaattatat atctaagacc gcttctctga
     6421 ttaaatacta tttgaggata aggtatggtt tcttttaaat ctatagtatc ctttgcagct
     6481 agtggatgac tatatggtac aattagtact aaatcttgtt tggcaacagg tataaattca
     6541 attccctttt ctttttcaag tttcgaacaa aatgcaatat catattttct ttctttaaga
     6601 ccttgaataa tgtcagtagt tacaccagta tgaaatttaa aatctattga tttagcagga
     6661 ttagatttta aaaatttatg cactatgtct gggataaaat cagtaccaag tgttctaaga
     6721 aaagctaagt ctatttgtcc ttcaccagta ccagttattt ttaatgattt tacacttgaa
     6781 tctaaaattt ctaatgattt ttccacatct tttaaaaata tttttccata cttagtaagg
     6841 actatatttc taccttcttt ttcaaataga gccaccccaa gttcattttc tagtaaagaa
     6901 atagcatgac ttaagcttgg ttgtgttata gataaatttt ctgccgcttt tgtatagtgt
     6961 tctaaatgtg ccaatgtaac aaaatagcgt aagtgatata aattcaattt ttgttcatcc
     7021 tttcataggt atttttattt atatatttgt agaagttaac ctttaagata tttttacatt
     7081 tttattatat atgattatag agtaagaata aaagctttta aaaaaattta tcgttttttg
     7141 tctgaattat aagataaata tatttatacg acctaataga aaaatacaag aattatctta
     7201 taaataaaat ctataaacaa aatctatgaa ttaataggaa attatcaatt tgttattagt
     7261 aaagtctcgt gatacaataa atttataaaa tctagataaa tagaaatcaa aaaatacttt
     7321 tatttttaag gtaaaataat actactagta ggataaataa aaatataaaa tcaaaaatac
     7381 tattaattat aaagaaaata aagggggaaa atttaagcta tgaataatca aaaaaatctt
     7441 aatattccaa ttacaattag ttcttataca ttgggaacag aagtttcatt ccgtgataga
     7501 gttcgtattg caaaagaagc tgggtttgat ggtattggat tgcgtgctga aaattatatt
     7561 gatgcaaaaa atgcaggtgt tacagatgaa gaaatgcttg caattcttaa tgaatatgat
     7621 atgaaagtta cagaagtaga atatataact caatggggaa gagaagaaga tagaacggaa
     7681 gctcaaagag aaaaagagca aaatgtttat catatggctc acttatttaa tgttaaacat
     7741 ataaattgtg gattattaga gatgatacca gaagaacaaa ttataactgc attgggagaa
     7801 ttatgtgaca gagcaggtga acttattata ggtcttgagt ttatgccata tagtggtgta
     7861 ccagatttag ctactgcatg gcgtgttgtg aaaggatgta atcgtgaaaa tgcaatgctt
     7921 atattagata cttggcactg ggctagagca aagcagacag cagaggatct tgcacctgtt
     7981 ccagcagata agatagtatc aattcaagtg tgtgatgttt tagaaactcc ttatgaaaaa
     8041 cttagggatg aatcactaca tgaccgtttg gctccaggtg aaggatatgg aaacacagag
     8101 gaatttgtga aaataataaa agaacacaat atatcaccaa gagtcatagg ggcagaagta
     8161 atatctgatt ctctaatatc taaagggtta tccactgctg ctgaaactgt atttaatgca
     8221 actaaaaaag ttttggataa agcatggtct gaagtatctc caaaataaac ttatataata
     8281 taaaaatata cagagtatta taatataaaa cttttaaata taaaacttga aaggaaggaa
     8341 aaccttatga agattagttc tatgtttaca cctataagaa ttggttcaat gacagttcca
     8401 aatcgttttg tagttcctcc tatgggaaac aattttgcaa acacagatgg tactttaagt
     8461 gaaacttcta aggcttatta tcttgagcgt gctttaggtg gttttggatt aataactata
     8521 gaatctagtg ttgtagacaa aaaagcaaaa ggaggtcctc gtaagccttg tttatatgat
     8581 gatagcacaa tagatagttt taaaaatgta atagatgctt gtcatgatgc aggttcgaag
     8641 gtatctatac aattacaaca tgctggttct gaaggaaatg aaaaagtagc aggacatcca
     8701 ttaaaagctg cttctgcaat acctgcatca aatggtcgta atacaccact agcaataaca
     8761 acagaagaaa tatatgaact tatagaatct tatggagatg ctgcattacg tgcacaaaaa
     8821 gctggtgcag atgctgttga agttcactgc gctcatggat atctggttag tagttttata
     8881 tcacaaagaa caaataagcg tgtagatgag tttggtggat gttttgaaaa tagaatgaga
     8941 ttacctcgtc ttatcattga aaatatacgt aaaaaagtag gaaattctct agcaatatta
     9001 tgtcgtataa acagtactga tgatgttcca ggaggaattg atgtacacga tagttcagta
     9061 atagcagcat atctagaaga ttgtggaata gatggacttc atgtttcacg tagtatacat
     9121 atacatgatg aatatatgtg ggctcctacg actttacatg ctggattcag tgcagaatta
     9181 gttactgaaa taaaaaaagc tgttagtata ccagtaataa cagttggacg ttatacagag
     9241 ccacagtttg ctgagttaat ggtcagacaa ggaagatgtg atttagtagc attcggtaga
     9301 caaagcttgg ctgacccaga aatgcctaat aaagctaaaa atggaaaact agacgaaatg
     9361 ataccatgta ttgcatgctt acaaggatgt gtaccaaata tgttccaagg caaacctata
     9421 gcctgtcttg caaatcctat attaggacat gaagcagagc ttaagccagc tgaaattagc
     9481 aaagaagttc ttgttgttgg tggaggtgtt ggaggtatgc ttgcagcatg ggtatgtgct
     9541 aaaagaggac ataatgtaac tttagttgaa aaatctgaag ttcttggtgg gcaaatgcgt
     9601 ttagctgcat atcctccagg aaaaggagat attactaact tagttagaag ttatatttca
     9661 aaatgtaatc aatatggtgt aaaaatatgt actaatacag aagctacagt agaacttata
     9721 aaggagaaat cacctgatgt agttattata gctactggag caactccact tgtacttcct
     9781 ataccaggaa tcaatgattc aggattaatt catgctgtag accttcttga tggtaaaaaa
     9841 tcttgtggga aaaaagtact tgttgttgga ggtggaatgg ttggatgtga agtggcagcc
     9901 ttccttggag aacaagaaca tgaggttact gttattgagc ttagagaaga agttggtgca
     9961 gatgtaattt cagaacaccg taaattcctt atgaatgatt ttgaaaatta taatatacat
    10021 accattactg gtgcaaaagt ttctaaattt tatgatgatg gtgtcgacta tttacttaca
    10081 gatggcacag agcatgattt aaaaggtttt gattcagtag tacttgctat gggttctcgt
    10141 aattacgacc cattaagtga agttataaaa gaagttgtaa aagaaactta tattgttggt
    10201 gatgcagtaa aagctcgtag ggcattagat gcaacaaaag aagcttttga agtagcatta
    10261 aatatataaa taataaataa atttttcttt gtaaataact gaataggtta tttgcaaaga
    10321 aaatgataat attgggagga gattatagtt atggaaattt caggtagaac aggattattt
    10381 gcattaatag gtacaccggt tggtcactct aaatcaccag tgatgtataa ttatagtttt
    10441 aagaaattag atttagatta tagatattta gcctttgata tcacaataga taaggtaaaa
    10501 gaggcacttt tagcaataaa aacttttaat ataaaaggag ccaatgttac tatgccatgt
    10561 aaatctgctg ttacagaata tatggatgaa ctttctccag cagctcgtat aattggagca
    10621 tgtaatacaa ttgtaaatga caatggaaag ttagttggtc atataacaga tggagtaggc
    10681 tatgttcgta atttaaaaga aaatggggta gaagttaaag gtaaaaagat tacaattatg
    10741 ggtgctggtg gtgcagcaac tgctatacaa gtacaatgtg ctttagatgg agcaagagaa
    10801 atatctatct ttaatcctaa ggatgatttt tataaaagag cagagcaaac agttgagaat
    10861 ataaaaaaaa atgtaccaga atgtgttgtt aatctatatg atttagaaga tacaaacaaa
    10921 ctttatgagg agatagaatc aagtgatata ttgacaaatg caactcttat tggtatgaag
    10981 ccttatgaca atgaaacaaa tataaaagat actagtgttt taagaaaaga tttagtagta
    11041 acagatgtcg tttataatcc taagaaaaca aaaatgattg aagatgctga agctaatgga
    11101 tgtaaggcta ttggtggatt aggaatgtta ttataccaag gagcagaagc ttttaatcta
    11161 tatactggat tagaaatgcc tgtagaagaa gttaatgaat tatgctttaa atagataaga
    11221 gtaatatctt aaacaatgaa aaaacatacg tatttcaata tttttaataa taaattacaa
    11281 attgtcatag tgggttttgc ctcatagtcc agttaaattt gaagctttaa ctgactatac
    11341 tttaaatcca ttatggggat gagtttattg tacacaaaaa tgttaataaa ttaacaagaa
    11401 tgggagaaaa attatgaaca ataaaaaata ttatcctact gccatagcgt tgtatttttc
    11461 atatttttta cttggtattg gaatttcaat tttaggacaa tacaaaccag aattttcatc
    11521 aatgtggggg gctaaaacat tatcagatgg aacattggat gtaagtattg ttttagctgt
    11581 tattgctgca ctaggattag ggagacttat atcttatcca tttgcaggac ctatttcaga
    11641 taagtatgga agaaaagtaa gtggtcttat aggaaacttt ctacatgcaa ttttctttgt
    11701 tggaattgta ttttcaccaa atttttatat agcatatgta tttgcaataa ttggaggggc
    11761 agcaaattca ttcttagata cctgtgtaac tccttcttgt atggagatat ttgctagttt
    11821 aggaacaata gcaaatatgt ttactaaatt tacaatagct ttagcacaat ttttgcttcc
    11881 ttttataatt ggatttgttg cagcaaattc aatatcattt aaagttattt ttatagtaac
    11941 agcgattctt attgtagtag atgccatttt gatagctgtg ttaccatttc caccagctaa
    12001 taatgttatt gataacaaag gcaaaacagt taaatcagag aagatgaaat ttacaccaac
    12061 aagtatagct cttgtttgta ttggatttac atgtacatct acatttgtat tatggttaaa
    12121 ttgtaatcag gaattgggta aattgtatgg gatggctgac cctacaaaaa ttcaatcctt
    12181 ttactcaatg ggagtaatat gtgcagtatt aataacttct ttacttatta aaaaatatat
    12241 taaaccaata agaatattag ttatatatcc tataatagct ttacttatgc tattagttgt
    12301 atattttgtt caaactccaa ctatttgtat gttaggggga tttgtaatag gttattcagc
    12361 agcaggaggt gttcttcagt taacaacgtc aactgcaaat gaaatgtttc caactaataa
    12421 aggaaaaata acatctatag ttatgattgc atctagtata gcaaactatg ttattttaaa
    12481 tatagcaggt attattacaa aatcaggggg agttaatgga ccaaaatatg ttgtattgtt
    12541 taatgtggca ataacatttg ttggaatttt acttgcattg tttgtaaaca tgagatatga
    12601 aaaagaaaaa gtgtatgatt atgatgtcta atattagtat aatatatgca tagagaagtc
    12661 taaatctagg atagattttt attaaaagta attaaaatta ataaggatat tatttacagt
    12721 tataagatag ggtatttaca taattaataa taaggatatt actagaattt caatttctag
    12781 taatattttt ttgcgtataa atgtaaactt gacgaaataa gtagtataat caatataaat
    12841 tacatcatgt aaaggaggat aatttggtgg agaatgtttt taagcgattg caggaattta
    12901 atggttatga tggatacaaa gaatcatttg aaatgaatta cctatgtata tatgaaagta
    12961 taccgttaag agaacaaata gagcttgcaa ataatctagt agatgaaatt ttaaatatgt
    13021 ataaaagtga atctaatgaa atttatttat tggaggatag taattctaag tctttaattt
    13081 gttatttcga aatttttatg aaaaagataa atactttggt aaaagaaatg attattgatg
    13141 agaaatggtt atataaatta actaaagaat taatatataa gagtaaaaaa gttgagtatg
    13201 taaaactagg gttagtttta agtgagaaat atctaaatgt agaaaactta agagaagttg
    13261 tagatacatt ttctaaatct ggagaatacg tattttatct gagtaatact attaaaaaac
    13321 ttgaatttta caatacatat ttatttaatt tatctaaaaa ggctacaggt tcaataaaag
    13381 tatttgctat tgttaatatg gaaaatttag attctaaaat aaattcctat ctaattgaag
    13441 atggttataa ggatacaaag tacgaacgat tattgatgaa ctatattatt tcaattgtag
    13501 atttgaatga atatcttgaa aaacgagatt tagataaaga aaagataaat aatttagca
//
LOCUS       DAGSHT010000093        13513 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGSHT010000093 DAGSHT010000000
VERSION     DAGSHT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.095"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            94..1620
                     /locus_tag="MJB72_002259"
                     /pseudo
     CDS             94..1620
                     /locus_tag="MJB72_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1914..2252
                     /locus_tag="MJB72_002260"
     CDS             1914..2252
                     /locus_tag="MJB72_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263363.1"
                     /translation="MEQYSFSSELIDVMVNASNDLNRVYEKITSSGTFSGLQGTFDTL
                     YEYGLMLEDLLEVMDLSLLGGNETKEIIQNIAIDLKNISDEDNIADKALDLMGNIGEF
                     TYYIETNYSK"
     gene            2387..2632
                     /locus_tag="MJB72_002261"
     CDS             2387..2632
                     /locus_tag="MJB72_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263364.1"
                     /translation="MRSVTVEDGEERFAIEKIYVKSLKRYEVRICLYRDCKDRMNKLI
                     PRPVDISPDKLSTLILVGIKAGILDDDFKQELIKGLS"
     gene            2930..3121
                     /locus_tag="MJB72_002262"
     CDS             2930..3121
                     /locus_tag="MJB72_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263365.1"
                     /translation="MKYTLKYGLGQLKFDVKIRLEDILRLSSAGEDNDEVRLAERSRE
                     INDIIGKLYLRPSETKKKI"
     gene            3210..4123
                     /locus_tag="MJB72_002263"
                     /pseudo
     CDS             3210..4123
                     /locus_tag="MJB72_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4304..4585
                     /locus_tag="MJB72_002264"
     CDS             4304..4585
                     /locus_tag="MJB72_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263366.1"
                     /translation="MELKTIRITFTVSSETSEDDLRKLCKDYMGDEYVVNDEGDFEIS
                     FYLNTVLGYNEVLYFMELIHMELEKSITLLGIRYKGIGGLQMKHILQEE"
     gene            4592..6253
                     /locus_tag="MJB72_002265"
     CDS             4592..6253
                     /locus_tag="MJB72_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263367.1"
                     /translation="MIRNCSKIIKNYGFKINSKNHVFDSNGNEICNFVPIVLGQYMIG
                     DGRDRRTVIVLMGISEDGNQLETINIDVSDIEHLKWVKNSWGFQYKVHSKMKNQFLYL
                     IEVLVINGTTKKLSSQIGWVKDNGKYAYLHTRGVIGESDKEVDIGDNLSNYFLPSEIV
                     DINRACKASLNLLEVADYEVTVPLLALVYLSPLLQAIKDVGKMPEFVVWLFGTTCSRK
                     TSLARVFLSHFGDFTNRLPATFNDTYASIEIKANAIKDSLNLIDDYAPKQTKKQKDAQ
                     DEIAEKAIRAYGDRIARGRVNSSIISQKQYIPQGMLLMTGENLIRGHSTVARLVALEL
                     DKDSVNLKLLKEMQDSVSLLGESMRGYIEWLLHGMNKDSSDIGGILIHNFDLYKEELE
                     KIEYTHTKGGHGRSIESCAWLLVGISSMLSYFEDKKVISEVEFNQYIQFAKDTMVNVL
                     IKNNNITKESSPIDEFLYTIKEAIDSNSIKISTLVDGNKLNDNDDDTYGYKDDKYFYF
                     HPDKTYSYVQEIQSKSGNYISLTKRGLIKLLREHSIIKVDSDGSP"
     gene            6388..6825
                     /locus_tag="MJB72_002266"
     CDS             6388..6825
                     /locus_tag="MJB72_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263368.1"
                     /translation="MTIRINLDGENILENVDNKFYGLRARVDFDLDLLDEDVIDIMDR
                     ILTLDVNPSRMFITKGRVDIFYQPQKEISIESKLGYMKLINEFIDFIESQEIVEMKFL
                     GWNLNGDKIVESQANDSNISFSKEIFNPDTVYLDGLIEGYVNL"
     gene            6932..7847
                     /locus_tag="MJB72_002267"
                     /pseudo
     CDS             6932..7847
                     /locus_tag="MJB72_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7877..8032
                     /locus_tag="MJB72_002268"
     CDS             7877..8032
                     /locus_tag="MJB72_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263369.1"
                     /translation="MTETYKISIGRKDLYSKDYKAVERIDYEKRYIVNISRSLVDILS
                     ILIEEGI"
     gene            8114..9679
                     /locus_tag="MJB72_002269"
                     /pseudo
     CDS             8114..9679
                     /locus_tag="MJB72_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            9812..10876
                     /locus_tag="MJB72_002270"
     CDS             9812..10876
                     /locus_tag="MJB72_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBY8263370.1"
                     /translation="MSKIEYYMNLPYDLSGSMSKNRFRNELLWGLKKMYELYKTNEDF
                     VVVFDYRCDIEVHLENKFEFYQVKTQNNSGTYTINKLTDKKKGSKSVVGNLYSLKYDI
                     NMNEIDDVYVAIVSNAPLNDGKKTYNNSHEVKIKTIDSSAIEKIKLSIKNDLNIDEDI
                     NLENLFFIRIGLDLIEPQKTLIGETAIFFEDVFGRECSKVKSLYNVLYEEIYSKSAYE
                     LDINTYENIVSKKGINRDRMDIILKSYANNTDKSVELAKYSINEWCKNNFSRRLSLIK
                     SLTTIVSKLSESSILKDIENCILSYIYNNIDKLQVSDKELIEIISEIVEEKRPIEVSK
                     TDIEALILLTLKKYEEGVYE"
     gene            10869..12542
                     /locus_tag="MJB72_002271"
     CDS             10869..12542
                     /locus_tag="MJB72_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263371.1"
                     /translation="MNSLFVNSLYIFEYESKMAKKIDFKHGINILTSDEKSGNDVGKS
                     VLLKSIYHTLGADSIFDHMWNKITKTYILNVTIKNKTYYVYRRDNLFKIYNTNFKKIF
                     MTNNRDELSNYLSDLYSFKVMLPKRENEELELAPPVYSYLLNYIDQDHLDGSKFSSFN
                     SLTQFPNYKENVIYTHFGLFDEEYYKAIKELEILKSEEKNKKKEREVTLNMLNKIKSY
                     LGNLDAPESIEVLNIELNKTKEEYSNIVINLQKIKNNLMKIRNEKVDLELNIKEIELT
                     RRNEMKKLKVKNVCPSCSHEIEDIDSKFIINNQIEDLIILKDELESEIIKCNRVIEKK
                     EEEYQSLLDKLHEYESNMKINDDSISNVLKHKAYIDTKDDIVLDLEIIDKKIKEIQQT
                     IEPYDKKIKEYNSLKKKANAKYEELMKKAKKDFGLEEIDENKIKNIKNNFIARGSNRA
                     ITTIMWYLTLLAVKYEFNENAIKFPLILDSPNNVESDEIKEESLFKYIFKNTKTDTQL
                     ILSSLGFNEMQFSEFKFDNIIKLENSKYNLLNKEDYYNNIDILNKIFEN"
     gene            12647..>13513
                     /locus_tag="MJB72_002272"
     CDS             12647..>13513
                     /locus_tag="MJB72_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263372.1"
                     /translation="MKDRFNLKRKSIQSLMNKNVDVYSCKDCIDDFIESNFKIYRSYY
                     ISDDDILEIMDGTGEDYKDVIADMLPDKAHIKSGEFGEITATEIYKSNIQTTEIYIVD
                     KLSHTRKEDTKVAAHKTDILIIEKTSEGFIIHSSEVKTKITDSDFNPIEAMIEGVNDD
                     IVTRVAETLNWIQRKLKTEKKFDELKFIKQVIESIENQNVENEYNGAVFIDESKLDYE
                     INKNIYCDVKLSKSQKGKYDNELKSIGCVIDGDKIRFDSVNIDDINKLNINSRKRNSI
                     EKLYNKSQRKLEH"
BASE COUNT         5949 a         1096 c         2446 g         4022 t
ORIGIN      
        1 ctttataact aagttatcta ataagagaca ttagtacaaa gttcattagt aagtgaacat
       61 aaagaattaa ggcaatggag agggagattg aaaatgacaa agtttaattt tataggaaat
      121 gaaatttata taacagaaga aagaaatagg tataatagca taagaataga atatgaaaat
      181 atagcgaata aagcaagaaa agaatttatt aaagtatata ggtcatgtaa tgaaaatcta
      241 gatgatgtaa ttaataatgc ttatgctcaa ggggcatcaa ttattttaaa atcaattaag
      301 tgtacactag atcgacttat agaaaataaa ttttataata taagcgaaga gttatttatt
      361 gaacaatact gccaaagagt tgtagaaatt tgggaatctg catacggtat aatcaatgac
      421 tagtatatga atattgtatt agatgaaagg caaaaggaag agtatagaca actaaggaaa
      481 aattcaagag ctagatggca aggtggaggt tttgggattc aaggggctat aaaaggagct
      541 acacaagcag gagcaatgaa tatggttaca ggagttgcac attctgcatt taatatgata
      601 agtaaaatag gtacgagtat aaaagtaaat aaacaaaaat ctaaaatatt taatgatcca
      661 tcgacattag atgtattatc tgaagtaatt tatttagcca ttttagatat taaatactca
      721 tatataaagt ttttagaaaa taatgcagga ttacagtttg ggtatataca tgaagaagag
      781 gaagaagaag caaatgtgat actttcaaat ataaaaggca gaaatttaga tgaagaagaa
      841 aaaataaatt taattaaggg acttatagat ttaaatccat acatggagtc tctatacaca
      901 tacatagtag ataactttgg tgatgaaaat atggaagttt ctaagatggc tagttatttt
      961 ggttttaata tagagccata caagcttagt ttagtagaga ataaattaat taatttagaa
     1021 actaacacag aagaagaaac aatattagct aagcagctta tattaaaaga agtaaataga
     1081 ttaggtatta atagtaaggt aaatggaatt gaagagttag accgaaaatt aaatcaattt
     1141 gatatagaag cgaggacagt agataacata ttgtttgaaa caagagaaaa agctaattta
     1201 gcaagacaag aaaaattaga tatagaaagt ataataaaaa atttagatga aaacaatgaa
     1261 gaaaatctag ttaatattaa aaaagagatt gaatctagaa attttaaaac agaaatagcc
     1321 gatttatata tagatagaat aaataatcat attaaaaatt tatattctga cactataaat
     1381 gaagcagaac aatatgagga taataaaacg aattttaaaa gtacgttaat aggattagcg
     1441 tttattgtac ctctaggtat atactttttt ggtaatgttg ggattatatt aaagatagtt
     1501 ataggtatat tcttattatc cgcagtatct gcattatttg aatcatataa aaaattaaag
     1561 gcaagtaagt attcattaaa acaacttaaa aagttaaaaa agtcaggtaa aataatataa
     1621 ttatcatgag gaatcaaatt gaaaggtata tttaataaaa cattgacaaa gattataata
     1681 agttcattaa tatttggaac tgtatgtagt agtatagttt atctaggtat gagaaatgaa
     1741 tttaacaata aattagtttt ttagaaaaag agatactaga cttaaaaaaa caaaacgtag
     1801 aattatctaa taaagaatta gaaaaagaat atgataattc agacgttaaa tctgaagatg
     1861 ttaaaaaaga tagagagatt actaatgagg taaaaaaaga atatgaaaaa gaaatggaac
     1921 agtatagttt tagtagtgaa ttaatagatg taatggtaaa tgctagtaat gacttaaata
     1981 gagtatatga aaaaataaca agctcaggaa catttagtgg attacaaggt acatttgata
     2041 ctttatatga atatggatta atgttagaag atttactaga ggtaatggat ttatctttat
     2101 taggaggtaa tgaaactaaa gagattattc aaaatatagc tatagattta aagaatatat
     2161 ctgatgaaga taatatagct gataaagcat tagatttaat gggtaatata ggggagttta
     2221 catactatat tgagactaac tatagtaaat aattaaatat taataataat agccttgcta
     2281 cacatgactg tagcaaggta ttatttttgt aaagaaagga agatttaatt tggctaaaca
     2341 aaataaggct caactatttg aaaaagaaac taaatattgt aagataatga gaagcgtaac
     2401 agttgaagat ggagaagaaa gatttgcaat agaaaaaata tatgttaagt cattgaaaag
     2461 gtatgaagtt agaatatgtt tataccgtga ttgtaaggac agaatgaata agctaatacc
     2521 aagaccagtt gatataagcc ctgataaatt atcaacttta atattggtag gtataaaggc
     2581 gggtatttta gatgatgatt ttaagcaaga gctaataaaa ggtttatctt aggaggtata
     2641 gaatgtcgat agaagaattt actagtggag taatgtttgg aatttctata tttggtacag
     2701 tattaaagaa gttattagat aataaaaaat agtacatagg aggtgttatt atgattggat
     2761 ttataatagg aactatgata ggagttggat tatgcattag catagaaatt atgataccaa
     2821 aaaatacaat agtatattaa cctatattta aactgttaat ataatcatat ataatttatt
     2881 tgagtcctta aagcccagaa aagttttata tgaaggagaa ttttataata tgaaatatac
     2941 actaaaatat ggattaggac aattaaaatt cgatgtaaaa ataaggttag aagacatatt
     3001 aagattgtca tctgcaggag aggataatga tgaagtaagg ttagctgaaa gatcaagaga
     3061 aattaatgat ataattggaa agctatattt aagaccgagt gaaactaaga aaaaaatcta
     3121 atagttatga agaaagtaaa aataaatcta aaagtaaata tgatagagat aaaattaagg
     3181 acatattaga agaagatgag ttgaatgtaa tggaaaagta tgtattttca tttgaatcag
     3241 gaaataataa gctatttaaa gagtattgga aagacagaaa gaagacagat ataccattaa
     3301 taacaacaat attttttaga actatgttaa gattaaagga tacatataga catgatttgt
     3361 ataacttatg ggaagattgt aatctaacaa cggaagaatt taaggaaaat atatataaat
     3421 cacaaaatga atctatagga tttattaaat ttaaagttga aaaaatgata gaagaagaaa
     3481 aagaaaggtt agggaaaagt attaataaca aatataaaag caatagaaaa ataacagaat
     3541 tgcagaatat tgatattgat aatatagtaa aagaattgat tatatctgat aatcctgaaa
     3601 ataaagaaat ctatcaaaag atgataatta ttacaaaatc attttttaag ataattgaat
     3661 atagaagaaa attatcatta agtaatcaaa cagtaactga taatttaatg gaagaggtaa
     3721 aaaaactata taatacatta ccaaaagaat cgagatatga tgcagcatat aagaatctaa
     3781 gaaagttaaa agaagatatt gaatttaaga taaaagaaat agataattat aaggagatat
     3841 ataaaagaga agttataaaa aattgtaaat tagttagtga gtataaaaaa aaagaagaaa
     3901 aactaaagaa gtctggttat atagaagaat cagaaaaaat ggcaaaaaaa tatatagata
     3961 tagaaaggca atttaatgag agtatgagaa aaaaatggat atatatatag atatgtatat
     4021 aagacgtaag gacttagaaa tagaagaaat ggatatgaaa aaatatgaag atgataatat
     4081 aacgaattcg atgattaaat tagcagaaga gattaactct taattagcat aaaaatagta
     4141 cacagttaag tgtgctattt tttttatgaa aatgaaaaaa aattggataa tggtttggtt
     4201 attttttaat aaaaaattag cttatattta atttcagaag gaggtaagga aataaaaata
     4261 cgggctataa gggctttagg acaacttagt aggaggaaca aaaatggaac ttaaaacaat
     4321 aagaattaca tttacagtaa gtagtgaaac aagtgaagat gatttaagaa agttatgtaa
     4381 agattacatg ggtgatgagt atgttgttaa tgatgaaggt gactttgaaa tatcctttta
     4441 tttaaataca gtacttggat acaatgaggt cttatacttt atggagttga ttcacatgga
     4501 gctagaaaaa agtattactc tattaggaat tagatacaaa ggaataggtg gactccaaat
     4561 gaagcatatt ctacaggagg aatagagtaa gatgattagg aattgtagta agataattaa
     4621 gaactatggt tttaaaataa acagcaaaaa tcatgtattt gatagtaatg gaaatgagat
     4681 ttgtaacttt gtaccaattg tattaggaca atacatgata ggtgatggaa gagatcgaag
     4741 gacagttatt gtattgatgg gaatatcaga ggatggtaat caactagaaa ctattaatat
     4801 agatgttagc gatatagagc atttgaaatg ggttaagaat agctggggat ttcaatataa
     4861 agtacattct aaaatgaaga atcaatttct atacttaata gaagtattag ttataaatgg
     4921 aacaactaag aagttaagta gtcaaattgg atgggttaag gataatggaa agtatgcata
     4981 ccttcatact agaggtgtga ttggagaaag tgacaaagaa gttgatatag gtgataactt
     5041 aagcaattac tttttaccaa gtgaaatagt agatataaac agagcgtgta aagcaagtct
     5101 aaatctatta gaggtagcag actatgaagt tactgtacca ttattagcat tagtatattt
     5161 atccccttta cttcaagcta ttaaggatgt aggaaagatg ccagagtttg tagtatggtt
     5221 gtttggaaca acttgttcaa ggaaaacttc tttagcaaga gtatttctga gtcactttgg
     5281 agattttact aatagattac ctgcaacatt taatgacacc tatgcatcta tagagattaa
     5341 ggctaatgca ataaaggatt cacttaactt aatagatgac tacgcaccaa agcaaactaa
     5401 aaagcaaaaa gatgcacaag atgaaatcgc agaaaaggct atacgagcat atggagatag
     5461 aatagcaaga ggtagagtaa actctagtat aattagtcaa aagcaatata tacctcaagg
     5521 aatgttgtta atgacaggag agaatcttat aagaggacac tcaactgttg caagattagt
     5581 agcattagaa ttggacaagg actcagttaa tttaaagcta ctcaaggaaa tgcaagatag
     5641 tgtaagtttg ttgggagaaa gcatgagagg atatatagag tggttgttac acggcatgaa
     5701 taaagatagt agtgacattg gaggaattct aatacacaac tttgatttat ataaagaaga
     5761 attagagaaa atagagtata cacatacaaa aggtggtcat ggtagaagta ttgaatcatg
     5821 tgcatggctg ctggtaggta tatcatccat gttatcatat tttgaagata aaaaagtaat
     5881 atctgaagtt gagtttaatc aatatataca atttgctaaa gatactatgg tgaatgtatt
     5941 aattaagaat aataatatca caaaagaaag ttcaccaata gatgagtttt tatacacgat
     6001 aaaagaagca atagattcta actctattaa gataagtact ctagtagatg gaaataaatt
     6061 aaatgacaat gatgatgata catatggata taaggatgat aaatattttt attttcatcc
     6121 agataagaca tatagttacg tacaagagat acaatcgaaa tcgggaaact atataagttt
     6181 aacaaagaga gggttaatta aattattaag agaacactcg ataattaagg tagatagtga
     6241 tggatctcca taaaagtata ctattaaagt aaaagatgga actgactata aagagaaaag
     6301 atctaggcta ttacgattag ctataaatac aatagagaaa ttataagaca ataagagata
     6361 aatggtgata tagaaagagg taattatatg acaattagga ttaatttaga tggagaaaat
     6421 atattagaaa atgtagataa taaattttat ggattaaggg cacgtgttga ctttgatctt
     6481 gatttattag atgaagatgt catagatatt atggatagaa tattaacttt agatgtaaat
     6541 ccatcaagaa tgtttataac aaaaggtagg gtagatatat tttatcaacc tcaaaaggag
     6601 atttctatag aatctaagtt agggtatatg aagttaatta atgaatttat agactttata
     6661 gaatcgcaag agattgtaga aatgaagttt ttaggatgga acctaaatgg agataagatt
     6721 gtagaatcac aagcaaatga cagtaatatt tcatttagca aagaaatatt caatccagat
     6781 acagtttatc ttgatggact tatagaggga tatgtgaatc tttagaaaaa gctaagttag
     6841 aagtatgtag ttctaatagg agctgcatac ttacttaata caagattatt gagaagttaa
     6901 aaaaaggaaa ttattatgga ggagaactta tatgaactat attacaacac aaggatttga
     6961 tacaataaaa agggataaga tagaaataaa aagaatggtg tatgctttag agaatgaggg
     7021 tgattcgtac aatgttattg aagaatgtat atgtacttta gaagaaaata tagatgtaaa
     7081 tgatgatttt gtgatagaag tagaaaatgt atttagtttt gttattgata gaataatgga
     7141 ctttataaaa gagaagaatt tgccaaaaaa caaaatagaa catgtaagag cttatataga
     7201 tgtatttgaa aatccaactt caaatacaaa aatagtaaag tctatagata tatatgaaga
     7261 ttatgataaa agatatattg ataataagtt ggataagtta tttaaaagca tggataatat
     7321 gataaaaccc gaatttgttc cagtaataga tggtaaattt gatatacgtg aaagtaagga
     7381 tttgaaagag tataataaaa aattaatgtt agacatagta aagcaaaaaa cttatgaagt
     7441 aaataatgga ggaataagaa taaataaatt atctgcaata ttaaaattag gagataaaag
     7501 catagatggc ataagcaaaa tgatgaagga tgtggcaaat ctaaagataa aaccaaaatc
     7561 agtatataag tgaagataca gttgttgtaa tgtatatatg taataagagt tgtattgtgc
     7621 ttaatataga agagtattcc aagatactgt cagattttgt tgactacata aataatatag
     7681 aagatatgga cttatattta gatgatagca ttttatatgg tgactataaa ataaattgtg
     7741 gagtagacga tagagatata attgatttta attatcaaag ctttaattca aaagtaactt
     7801 tgtttgctgg acatatagga aacgagataa tacaagctgc attatagaaa tatacaatcg
     7861 tagagaggat atagatatga cagaaactta taaaatcagc ataggaagaa aagatttata
     7921 ttctaaagac tataaggcgg ttgaaagaat cgactatgaa aagaggtata tagttaatat
     7981 aagtagaagc ttagtagaca tactttctat acttatagaa gaagggatat aaattataga
     8041 tatggctaca tacttagtgt gtagccttta atatagactt aaattagtat atttaaactt
     8101 tggaggatat aatatgaaat caaagaaagc agtagtatat gcaagattta gtttggacaa
     8161 tcaaagggat gagtcaatag atgctcaatt aagggcaatc aatgaatatg aagataagaa
     8221 taatataaaa atagttaatc agttcataga tagagcaaaa tcagcaacat cagataaaag
     8281 accagaattt caaaatatga ttaagttctg tgaagcagat acaacaggaa tatctatggt
     8341 aatagttcat aagctagata gattttctag agataagtat gattcagcta tgtataaaca
     8401 gaaattaaaa gtaaaaggaa taagagttgt aagtgtatta gagaacctag ataattcgcc
     8461 tgaatctcta atattagaat cagttataga aggtatggca caatactata gtgctaactt
     8521 agcaagggaa gttgctaaag gtcaaaaaga aaatgctctt aaagctctac acaatggagg
     8581 agatgcacca ttaggatatg atgtagcaca tgataaaact tatacagtta atgaagaaga
     8641 agcacaagca gtaaagatta tatttgatag atatattaaa ggatattcat atagtaagat
     8701 aatagatgaa ttaaatgatt taggatataa gactaaaaga ggtaataaat ttggaaagaa
     8761 tagtttacat ggaatattga ataatgagaa gtatacagga gtatatgtat ttaataagac
     8821 tcagagaaaa ggtgtagatg gaaaaagaaa tggtcataag caaaagagtg aagatgaaat
     8881 aataaaggta gaaggtggta tgccagttat aattgataaa gaggtatttg aacaagcaca
     8941 agagatgata caaaaaagaa agaaaactcc aggagcacat aaagctacta ctacatattt
     9001 attaacagga ttaattaaat gtggagaatg tggacatgca tttcagggga ataagagaaa
     9061 agactcttat ggtaatgaat atgtttcata tagatgtgga tgtagaaagc aaaaacgaga
     9121 ttgtaataat agagaaataa aacgagatta tctagaagag tttatattac aagaacttga
     9181 aaagaacata ttaaatgatg aggcaatacc agctttaagt aaagcattga atgagaagtt
     9241 gaattataag aatgaaagta tgcagagtta cttaaaaact tagatcagaa actagagaag
     9301 gtaaataaag agataagtaa tatactaaat gcaataatga atggtatagt aagtagcatg
     9361 ttaaaagata agcttgatga gctagaacaa gtaaagttaa acttagattt aaaaatgaat
     9421 gaattaaaga tagaaaataa agttgttaga tggtgtaggc gtaacagaag aacaaataag
     9481 aagtatgttc tcaagattta aggaatttgt gctagagagg aacatacctg aatgtaagaa
     9541 atttataggg gaccatataa aggaagtaat cgtatataaa gatcacgttg aagttgtatt
     9601 caatttggtc ttttcttttg tcgatgatga aatagctcat aacttacaca ttactgtatc
     9661 aagagtaaaa gtaaaataaa aacattaaat ataaatgtta atttaaatga tattatggat
     9721 atatttaaca aataagtagt gttacatgat ataatatcac atgtatatga aagggataaa
     9781 atttatacta ttagtattgg tggtgaaatg aatgtcaaaa attgaatact atatgaattt
     9841 accctatgat ttatcaggat caatgtctaa gaatagattt agaaatgaac tactttgggg
     9901 attgaaaaaa atgtatgagt tatataagac gaatgaagat tttgtagtag tttttgatta
     9961 tagatgtgat attgaagttc atttagaaaa taaatttgaa ttttatcaag tgaaaactca
    10021 aaataattca ggtacatata cgataaataa acttactgat aaaaaaaagg gtagtaaatc
    10081 agtagtaggg aatttatata gcttgaaata tgatataaat atgaatgaga ttgatgatgt
    10141 ttatgttgca attgtatcaa atgctccttt gaacgatggt aaaaaaacat ataataattc
    10201 tcatgaagtt aaaataaaaa caatagatag tagcgctata gaaaagataa agttaagtat
    10261 aaaaaatgac ttaaatatag atgaagatat aaatttagaa aatttatttt ttattagaat
    10321 tggtttagat ttaattgaac cacaaaaaac tttaattggt gaaactgcta tattttttga
    10381 agatgttttt ggaagggaat gtagtaaagt aaaatcatta tacaatgtct tatatgaaga
    10441 gatatatagc aaatcagcat atgagttaga tataaatacg tatgaaaata tagtatctaa
    10501 gaaaggtatt aatagagata ggatggatat tatattaaaa agttatgcta ataatacaga
    10561 taagtcagta gaactagcaa aatatagtat aaatgaatgg tgcaagaata atttctcacg
    10621 tagattatct ttgataaaat cattaactac aattgtatct aaattaagtg aaagtagtat
    10681 tttaaaggat atagaaaact gtatattgtc atatatatat aataatatag ataaattaca
    10741 agtatcagat aaagagttaa ttgaaattat aagtgagata gtagaagaaa agaggcctat
    10801 agaagttagc aagacagata tagaagctct tatcttacta acattaaaaa agtatgagga
    10861 gggtgtttat gaatagtttg tttgtgaaca gtctatatat atttgaatat gaaagtaaaa
    10921 tggcgaagaa aatagacttt aaacatggga ttaatatatt aacatcagat gaaaagagtg
    10981 gtaatgacgt aggaaaatca gtactattga aaagtattta tcacacttta ggagcagata
    11041 gtatatttga tcatatgtgg aataaaatta caaaaacata tatattaaat gttactataa
    11101 aaaataagac atactatgta tatagaagag ataatttatt taaaatatat aatacgaatt
    11161 ttaaaaaaat atttatgaca aataaccgtg atgaattatc taattattta agtgatttat
    11221 atagttttaa ggtaatgctg ccaaaaagag aaaatgaaga attagagtta gcacctcctg
    11281 tatatagtta tttgcttaac tatatagatc aggatcattt agatggatct aaattttcat
    11341 cttttaattc attgacacaa tttcctaact ataaggaaaa tgtaatttat actcattttg
    11401 gactttttga tgaagagtac tataaagcta taaaggagtt agaaatatta aaatcagaag
    11461 aaaaaaataa gaaaaaagaa cgagaagtta ctttgaatat gctaaataaa ataaaaagtt
    11521 acttaggaaa cttagatgca ccagaaagta tagaagtttt aaatatagaa ttaaataaaa
    11581 ctaaagaaga atattcgaat atagttataa acttgcaaaa aataaaaaat aatttaatga
    11641 aaataaggaa tgaaaaagta gatttagaat taaacataaa agaaattgaa ttaacaagaa
    11701 ggaatgaaat gaagaagtta aaagttaaaa atgtgtgccc aagctgttca catgaaatag
    11761 aagatataga ttctaaattt ataataaata atcaaataga agatcttatt attcttaaag
    11821 atgagttaga gtcagaaatt ataaagtgca atagagttat agaaaagaaa gaggaagaat
    11881 atcaatcact attagataaa cttcatgagt atgagtcaaa tatgaaaata aatgatgata
    11941 gcatatctaa tgtcttaaag cataaggcat atatagatac taaagatgat atagttttag
    12001 atttagaaat aatagataag aaaataaaag aaatacaaca gacaatagag ccatatgata
    12061 aaaaaataaa agaatacaac agtcttaaga agaaggcaaa tgctaagtat gaagagttaa
    12121 tgaaaaaggc taaaaaagac tttggattag aagagataga tgaaaataaa ataaaaaata
    12181 taaaaaataa ctttatagca agaggtagta atagagcaat aactactata atgtggtatc
    12241 tgacattgtt agcagtaaaa tatgaattta atgaaaatgc aataaagttt ccactaattt
    12301 tagatagtcc aaataatgtt gaatctgacg agataaagga agaatcattg tttaaatata
    12361 tatttaaaaa tactaaaaca gatactcaat taatattatc atcgttaggg ttcaatgaaa
    12421 tgcaatttag tgaatttaag tttgataata taataaaact tgaaaatagc aaatataatt
    12481 tattaaataa agaggattat tataataata tagatatttt aaataaaata tttgaaaatt
    12541 agtataggaa ttgtgggtat gatttctgtg aatcgtctcc accattgaaa tccacgaaaa
    12601 cacattgata ttttatcaat gtgtttttta agtaataggg ggaaatatga aagatagatt
    12661 taacttaaaa agaaaaagta ttcagtcatt aatgaataaa aatgtagatg tttattcttg
    12721 taaagattgt atagatgatt ttatagaatc aaattttaaa atttatagaa gttattatat
    12781 atcagatgat gatatattag agattatgga tggtacagga gaggactaca aggatgttat
    12841 agcagatatg ttaccagaca aagcgcatat aaaatctgga gaatttggtg aaataacagc
    12901 aacagaaata tataagagca atatacaaac aacagaaata tatatagttg ataaattaag
    12961 tcatacaaga aaagaggata ctaaagttgc tgcacataaa acagatattt taattattga
    13021 aaaaacctca gaaggtttta taattcattc ttctgaggtt aagactaaga taactgattc
    13081 agactttaat cctattgaag caatgataga gggggtaaat gatgatatag ttacacgtgt
    13141 tgcagagaca ctgaattgga ttcaaagaaa actgaaaaca gaaaagaaat ttgatgaatt
    13201 aaaatttata aaacaagtaa tagagagtat agaaaatcaa aatgtagaaa atgaatacaa
    13261 tggtgcagtt tttatagatg aatcaaaatt agactacgag ataaataaaa atatctattg
    13321 tgatgttaag ctttctaaaa gccaaaaagg caaatatgac aatgaattaa agtctatagg
    13381 atgtgtcata gatggtgata agattaggtt tgatagcgtg aacatagatg atattaataa
    13441 actaaatatt aatagtcgta aaagaaattc aatagaaaaa ttatataata aatctcaaag
    13501 aaaactagaa cat
//
LOCUS       DAGSHT010000094        13431 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGSHT010000094 DAGSHT010000000
VERSION     DAGSHT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.096"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(329..1210)
                     /locus_tag="MJB72_002273"
     CDS             complement(329..1210)
                     /locus_tag="MJB72_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBY8263373.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1666..2640
                     /locus_tag="MJB72_002274"
     CDS             1666..2640
                     /locus_tag="MJB72_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263374.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     ISMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2756..3817)
                     /locus_tag="MJB72_002275"
     CDS             complement(2756..3817)
                     /locus_tag="MJB72_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY8263375.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(4429..5280)
                     /locus_tag="MJB72_002276"
     CDS             complement(4429..5280)
                     /locus_tag="MJB72_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8263376.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5297..6217)
                     /locus_tag="MJB72_002277"
     CDS             complement(5297..6217)
                     /locus_tag="MJB72_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBY8263377.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6327..7241)
                     /gene="murQ"
                     /locus_tag="MJB72_002278"
     CDS             complement(6327..7241)
                     /gene="murQ"
                     /locus_tag="MJB72_002278"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBY8263378.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7340..8437)
                     /locus_tag="MJB72_002279"
     CDS             complement(7340..8437)
                     /locus_tag="MJB72_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBY8263379.1"
                     /translation="MGRLGISIYPEKTASKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(8670..10016)
                     /locus_tag="MJB72_002280"
     CDS             complement(8670..10016)
                     /locus_tag="MJB72_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8263380.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(10105..10413)
                     /locus_tag="MJB72_002281"
     CDS             complement(10105..10413)
                     /locus_tag="MJB72_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8263381.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10675..11064)
                     /locus_tag="MJB72_002282"
     CDS             complement(10675..11064)
                     /locus_tag="MJB72_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263382.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKQMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(11066..12226)
                     /locus_tag="MJB72_002283"
     CDS             complement(11066..12226)
                     /locus_tag="MJB72_002283"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBY8263383.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12657..>13431)
                     /locus_tag="MJB72_002284"
     CDS             complement(12657..>13431)
                     /locus_tag="MJB72_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263384.1"
                     /translation="LVDGQSFEENLIKDIIENLKIKNNSDIETYKQSLKELDEYIAKW
                     VHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTMFEDVFINK
                     FNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIINNAIKYSKE
                     VGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYKSTGMGMYF
                     VKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         4328 a         2360 c         1463 g         5280 t
ORIGIN      
        1 tattcctatt tatataattg tttcttatta taattaatat actttttaat caaatttaaa
       61 cagaataatt ataccaatat ataaaaaaat ttctaaaaaa caataaaatt aagttataaa
      121 caccaatata tttctattat ttcaataagt atacaattag attagaatct aacgctagta
      181 ataaaaaatt actttcccaa aatagatata agcctcattt tatcgttcaa ataatgaaaa
      241 tatatttgat ttcatgcaaa aagagtgcct aatgaactaa aaagttcatt ttgagacact
      301 cattttttta tataaattta tatagttttt aatcgttcgc attcaaccac tcagttaaaa
      361 cttttatata tttctcattt tcttcaacga atggcatatg tcttgaatac tcaaataatt
      421 cccacttact attaggtatt ctatcataca tagtctttgc aatatatggt gaacataaat
      481 ctattgctcc actagttact agacaaggct cttttatttc atgtaatcta tctgtaaatt
      541 catatccaga aagagttcct gtaggagaaa attcattttg tccccatcct acaatatatg
      601 cttcagaacc tgatttttta ggtcttctca ggcactctgg agaatcttct gtcacttcac
      661 ctgcacaata catttccata aatctttcta atgcatcatt atattctttg ctagagtaat
      721 ctccagtatt tactgcatca agtaatgctt tttggtcaac ctcactcata tatgatattc
      781 ttcttttttg ttctttctcc caaagttttg cagaagaaag agtagatgaa aggatgtaag
      841 acttaatacc ttttggttta tattcaatag cataccaaat agcctgcata cctccccaag
      901 attgacctaa cagatgaatt tcatcaagtc ctagatgttt tcttagttca ataagctcct
      961 ctatccaagt atctgcatta aatagctcag gatgcccttc tacaaatgaa tttccacaac
     1021 caatctggtc atacattatt acttgtctac cactttcagc tatcttatcc aatacctcaa
     1081 aatagttgtg tgtagaacct ggacctccat gaagtaatac taatggcttc ttcccttccg
     1141 tattttctcc tactatacgg taatacgttt taaaaccttt aaaaggcata tatccttcag
     1201 taattttcat gtttatctcc tccaatttgt aattataaac aataatcaag ttcattaaca
     1261 atactattta aaacctctta tacatatttg attatgtatt ttaatcttta tacctacttt
     1321 tataaatagt ataacataaa agttaaaaat ctctatacta aaccaattat aaaattatat
     1381 taactatata attttatttt ttaataatag tatatacata tttgattatt ttcatttgct
     1441 atttaaaatc aattttaagt tttcttctta taataaactt cctctttgtg gtctcatgtt
     1501 agtctctaaa tttaaaattt tacaaaaaat tttcataaaa taaaattata ttgattgtat
     1561 ctaaacaaat agggcaataa aataattaag tatagttact tacctttcaa gcaatattag
     1621 ttttatgtta gaattttatt atattgattt acaggaggtt tgcatttgaa taatattaat
     1681 tcacttaaag taataaaatt atctcttgga tgttctcttg ctatatttat tgcttggcta
     1741 ttaaaattag aatactcaat ggttgctgga gttatagtat tacttacagt aaaggacact
     1801 aaaagagaaa ctcttaaagg ctccattgga aagatttatg gttttctttt atgtactatt
     1861 ttttcatatt tatgttttaa tattttagga tataatttga cttcattttc aatatttata
     1921 tttataatta taccactttg ttttttgtta aatattcaag atgttattgc tatgtgtgta
     1981 gtcatagcat cccattacta ccttcaagga gaaacttcta tcaaatggat tttaaatgaa
     2041 gctggaatat ttgcagtagg aactggaata ggtgtcttaa taaatatgta tattccaaca
     2101 aatatacata aaatccatga agttcaaaaa aagttacagg aagaagtgag tattgtactt
     2161 atagacattg cagatattat tgtaaatcct aaaaaagaaa atggatatag tagagattta
     2221 tatacattaa attcacttat tgacagcagt atttctgaaa cttacgataa tattaataat
     2281 actttattaa gtgatactag attcttctta gaacatatgg atattataaa aagccaacgg
     2341 gatatcttag aaaatttgta tagttatgta tctcaactaa attctacacc accacaagct
     2401 catattctat ctgcttttat acataagata ggttatactg aatttgaagc tgaaacaggc
     2461 aatcttcttc ttgaagaatt aaagagacta atgattagta tgaaaaacca gcctctgcct
     2521 gttgacagaa cagagtttga aaatagagct attttattcc tatgcttgac tgagttaaaa
     2581 cagtttttag ttaatcgtaa acatgctcag atgcttagag ataataattt ttataaataa
     2641 aaaggagaga aatctagcag ttgacctgac tacaaaatat cagatataat attaaatgcg
     2701 cccctagtaa gagggcgcaa tgtttataac ttattctact gtcatgatta aatcattatt
     2761 catatcgaac atacattatt tgaccaagtc caatattagc tttttcatca gtgatttcta
     2821 ttttaatagt cacatcattt tttttacagt acttcgctaa ttctgcacca gaacgatttt
     2881 taccactaaa atctaaagaa aatggcttat catatttaaa aggatattca acaggtttac
     2941 cctctggtgc taatgcaaac atcccatatg gtgtgttcat aaatcctatt acagcaatcg
     3001 taagatttga tgggtctaaa tttgattcac tacctgtaaa tgtaattgta tttactgatg
     3061 aattaactac actacatcta acaccttcat acattacatg tttttcattt atatcttctc
     3121 ctttttttcc aaaatttcca acagcctttg ccccttcttg atgtggtaac atagtgtgct
     3181 tcccaggtgg tgctggtttc tgtgtctgtt ctggttttac cgtctgtact attgcaccaa
     3241 cttttggata tgctgtctta tcaaatggta atgggtggct aagactcttg tatatgatat
     3301 cctttcctgc tgatttatta gcatcaatat ctacatgata caaacgccca tcaccaaaac
     3361 ggataagatt ccactggtgg tcatttgatg tgctgtgtac ataataagat tcaattccca
     3421 tagcatcaca caaagctttc atgctcctag ccattcctga gcatgcacct tttttagtaa
     3481 ccaatgtata ggcagtattt gctccctcta ctgcattcca gtcaacatat gttgtgttat
     3541 ttttaatgtg attgtaggca actaaaatct tagcatctgt ggtcatattc tttgtgattt
     3601 tcttgttcat aaaatctgct gttgcttgtg ctactgagtt tagttgggct tgtgacagct
     3661 ttggaccata tacgctattg gttccagtga catagctttt tggtgttttt gctgttacct
     3721 ctttggctgt cataccataa actgtcatac tcatacctga gattaaaact attgttgtaa
     3781 tcaataactt ggtagctaac ttcctaattt tcttcatact taatattctc ctttataaaa
     3841 cattgttact tcctattctc taccattaat aaaattaatt taggatttaa aaaggtggat
     3901 tgtcaacatg tatatttctc cattcataca ttcgtgaaat atttaaattt catggaaaat
     3961 ctaatagata ttttctagat aatttgtata caatacaact cgcaaatcag ggtgtttgct
     4021 gttagtttaa gaaaatgatg atgattaata cattcggaat ctttgaccta taataaatac
     4081 cattgtcttc aatcattaat aatggtaaga gtaaagagaa ttatcaataa tacagttaaa
     4141 atcaactttg tatacatttc atacattctc tcccaacaac attatacttc ttataatttg
     4201 tatattcaat acgttttctg ggaaaatata ggatatatct ataaaaatat aaaaaaggag
     4261 ttgaacttaa aatttgatta aaaaaaatca attttaagac aactcatttg tattataaaa
     4321 atgctatata ttattattaa gtaagtcaat attttagata tttatctatt tcttattgac
     4381 ttacgcttat agtctaactt atttgtttag ttgatatata acctagtttt aagcattatt
     4441 aatatttaaa taatcaagta tcctttttgt ttcttgtagt ttctgttctg cctcatttaa
     4501 gttttcttga attatagaaa tatatattat atctatcaac gtaagcattg ctattcttga
     4561 agaaatagct ccttctctaa gccttttttc tatatttggt actactagct ttaaatcact
     4621 aatcttacta agatgattgt cactatattt agttatacaa atgaccttac atccttttaa
     4681 ttttgcattt tctatagact ttattacttc ttttgattct ccagagtaag atattgctat
     4741 agctatatct tctttatcta catttgaaga taccataagt tgtgtatgac tatctaaaga
     4801 ggtaaatgcc tgtttattta tccttaaaag tttcatctgt aaatctagtg caactaaagc
     4861 cgaaccacct actccaaata tatatacatt tttagccttt attatctgtt caactgcttc
     4921 ttttattata tctttttcat tcaacaacct tgtttggttg atagtctcta tgtttttgtt
     4981 tgtaattttg tttataacac tatctatatt acaatcatag ttttcatcta catatgtgta
     5041 ttcattttct ttgcccatca aatctttagc tatctcaatt tttaaatccc caaaacccga
     5101 atatcccatt tttctagaaa acctaactat acttgctgta cttgtttttg agtttgacgc
     5161 caattctgat acagaaagct tacttatagc ctctgtatta ttaagtatgt aatcagctat
     5221 ttttttatct acttccgtta aatcttcata tatctgtttt aattttaaaa tgcaactcat
     5281 actctatcac ctctacctat ttactataat atttcttctt agcaagtaaa catgctccta
     5341 aatctggagt caacactggt ctcactatac caaatctcgt tttattcaaa tattttttaa
     5401 gtggctctaa tattaaatct cctgacttaa acactccacc tgaataagat acctttatct
     5461 ttgtaccttg ttcaaaatga tgctctaatc ctattataag cctacttaat tcataagctg
     5521 catcatcaaa tattttacta gcagtattat caccttcaat aactgctttt aaacatatcg
     5581 tagcaaactt tgctatttct attctatctc cttgtatttc atcatttaca tattttatta
     5641 tgtcataatc attctctata ttaagatgtc tttttattaa atcatataat tttgtttttg
     5701 aacaccttcc atcagactgc attgtgaaca tttttaaggt agccattcct atataatacg
     5761 ctgaaccttc atctcctaat acatagcccc accctccaca tctgtgaaga ttcccatctt
     5821 tatctaaagc ctgtgctatt gaaccagtac ctgcaattat atttatacct ttttccccat
     5881 ttaaagaacc tgctaatgct atttctacat cattacctag agtataattt atatgactat
     5941 atgcattttt tgttgctact tctaatgcat acaatacttc ttttattttt ccatatccag
     6001 caagtccaac aaatgcatac gttatttctt ctacatttat cttagcatct ttacaaactt
     6061 cttccaaccc agttattaat aatttcgtta gattatcaaa tcctatttga ttataatgac
     6121 aagttccttt ggttatggtt cctactatat ttaattcttc atctactaat gtaaatgttg
     6181 ttttcgttcc tccaccatct acacctaaaa aatacatact atcctcctac aatatcttta
     6241 ataattcttt caatacttaa tatctaaaaa taaccttcca atgctgtttt tttaatattg
     6301 aaaggttatc agtactatta tttattttac aatttttcta ttgattttaa agcttcactt
     6361 atataacctt cttgtctatc taggatactc tttgcactat ccttatctaa accacttttt
     6421 atcataaata tagccaattt aacgctatta tctgcttctt ctaagaattt ttctgcaacg
     6481 tcatatttta catttgtagc tatcattatt attctttttg ctctttcaac caacttttca
     6541 tttgatgtct taacatctac catcaagttt gagtatactt ttcccatctt aaccatagaa
     6601 gctgttgtta acatattaca taccattttt tgagctgtac cacttttcat tctagttgac
     6661 cccattatta cttctgcccc tactataatg tttattggta aatccgcttt tttactcatt
     6721 tcactattcg gattcatagt tattgatagt actgcacacc ccaattcctt agcatactcc
     6781 attgcaccta taacataagg agtccttcct gatgcagcta taccacatat aacatcctta
     6841 gatgtaactc ctctatctat tatatcttgc tttccaagtt ctttatcatc ttcagcacct
     6901 tctattgctt taaatattgc ttgatttcca ccagctatta tagcttgaac aagctcaaaa
     6961 tccactccgt aagttggagg acattctgtg gcatctagta ttcctatcct cccactggta
     7021 cctgctccta tatagaataa tctacctccc tgtttcattc tatctactat tatattaaca
     7081 gcttttgcta tactctcctt ttgttcttca acagcatagg ctacagtttt atcttcgtta
     7141 tttattaatt ctattatgcc taatgtatct tctttatcta tttgcaatgt attcttattt
     7201 ctaccttctg taactaaatt ttctaaagtt tttattaaca tatcatttcc tcattttctt
     7261 aatattaaat ttttaaacac attttaattc caactttcat aattatattt ttataataaa
     7321 ttaatatttt cataataaat tattttgttg agggtttaaa attaaacttt tgccatggtt
     7381 taatataatc taatataaat aattcttctt ttcttatata tcctactaca ttcgatttac
     7441 cactgttttt catatcagtc aaggctattt gtaactcccc tgcataatga ccatattcac
     7501 tactttcaat tactatatct cctcttttta ttattttagg agtattaaat agttcaaatt
     7561 tgtatccttt atatttaaca cgagattgag tagaccttat cagactatca gatatatccc
     7621 ctctattaaa atgtatttct tcaaagagta tcttcttctc aacttctcgt atattttcct
     7681 ctattgctac atcaaatgta accatatctt ttctcataga acctaatacc tttagctcct
     7741 catatgttgg ataacaattt gatattatta tatcatttat attattcata gctattatat
     7801 gctttacttg aatatctata ggtagatttc tgtgcatctc aattgttgga agcccatcag
     7861 ttacaggcca tggtccaaat gtagtttcat tttgacttgt tacaaaaact gctgttctta
     7921 atccatactt attaaatctt tctgtacatt ttataaagtg ttctaaattt aatccactat
     7981 atctatgtgg ataaaagtta tgacatccta tcaaattttc tttatttggt tggtagtcca
     8041 ttattgtatc tatataatta gtattattac tcatattgat ttctatttta agattttgag
     8101 gattaaaagt cattaatgcc tcttcagaac ctgtaaatcc catatcaagc cttattccgt
     8161 ctgccttcgt ctttttaaaa aaagtcaaat ctttgtagct tatttctaat tcaccaaata
     8221 ctctgggatt tacatcaagt attacctcaa aacctttaaa cttagcgtat tcatttatac
     8281 ttttaaattt tttaattata ttttctttag tatctttaac tgataataaa catgaaaata
     8341 tacgtgtaaa tccattttca gcagctttat ctatataatc atatatttcc ttagatgctg
     8401 ttttttctgg ataaattgat attcctaatc ttcccatctg tgtcatctcc ttatatccta
     8461 tatgtgttta aatttatttc gaattacacg aaactttttt aaaaatttgg tgaaaaaaag
     8521 tttcatatat atttataaaa aaagaaaagt ttaccttttc tttttttata aacaatcaac
     8581 aaaatatctt cgttacttat ttcataactg ttatactttg aaatttatac taatctaata
     8641 tagtttttaa tttccaatat agtttctaat taataagctt cttgctcttc aactttttct
     8701 tcatgaagca acctcttatc gtacatcttg acaaatggat agtaaattat tattgatata
     8761 gctatacata ataagttaag aactgaagct ctccaatctc caccagtggc taaatatgcc
     8821 cctactggtc ctggtaatgt ccaaggagca gtaactacta ctctggatac taaattagca
     8881 ctcatagcaa accatgaaac tgttgccatt actattggtg ccacaacaaa tggtattatt
     8941 aatattgggt ttaatactat tggtgctcca aatattattg gttcatttat attgaatatt
     9001 gctggtatta cagttgcttt tcctaaagac tttccataag ctgattttgc tctaaatgca
     9061 agaagtaaag ctaagcctat tgtagctcca gaacctccaa tccatataaa ccattgaaaa
     9121 aagggttcag cagcaatacc tggaactgct tgacccgcag catatgaagc tgtattagca
     9181 tctaataatt gtatccataa aggtcttgcc aatgaaccta ttattgatac accatgtatt
     9241 ccaaatgacc aaaaaaacgt tattaaaaat attagtataa ttactgatgg taatgtgtca
     9301 gtggctgaaa ctaatggagt tactaattta gctatgaagc catgccaatc aaaccctagg
     9361 aaataagtaa caatagccat taaaattatt attaatgcgg ctggtgtcag agcttcaaaa
     9421 gaccttgtga ctgatgctgg aactgcttct ggcatggtta tcttgaattt agatttgtca
     9481 gtaaatctat aaatctcaac acctattata gatgttacaa tagcaacaaa cattgaacct
     9541 ccaccaagat tagccattgg catttgccat ccttgaggaa ttgaattata ccaagctaaa
     9601 agttctggac tcttttcaac tatctttaat aaatcagggg taagaccctc tattgcagta
     9661 ggaactattg tcaaaaggaa tgtagctgta gctaataaac ctcctgtcaa tccatctaac
     9721 ttatatgact ttgccaaact ataacctatg ccaaatgtag aatatactga tataataaaa
     9781 aatgtcattc tatatggtag taatattgta actgcatttt cctttagata ctgagttacc
     9841 ccccatgaat ctggtaatgg aggaaatgct aatatcaaga aaaatgaccc aactattata
     9901 agtggtaatg aagctacgat tccatctctt acagctctta agtgacgttg ttctgccaat
     9961 ctggccatag aagttgataa tttagtgtct aaaaattctg taaatttatc tagcatcttt
    10021 tctctcctta attaatattt atttttatta tcattaaaat ttttataaaa tcaactttct
    10081 tctaatctaa caacactgca actactattt actcaacagt tcttgaactt gttttaataa
    10141 ctttggtcct cctaatggac tataagcttg tgcttgtatt aatgcacatg gtacactaac
    10201 ctcatcagca aaagctttta aatagtcaaa tctatgtctg acttgtggag ctaccatagc
    10261 aatatcccat ccatttttaa cttcatcctc aaactcttga gtaccaacag ccaaaacatc
    10321 tatatctatt ccattttttg ctccttcttt ttgtaatgca ttaactgcta tagcacttga
    10381 catccctgat gaacaaacaa ataatactct catattgatt ccccctgttt ttttatttct
    10441 tttcaatcat tatagcatgt atttttgatt acaggaatac gttcgtatat attttttcat
    10501 attttacatt ttaaattgta attttatttc atgcatttat atcttgcttt aattgcttta
    10561 tttatctaat cattcttttg tctaaaatta cacactactt aaaatactca tatcttttct
    10621 taaaaaagaa aaaagactat cttattatta acataaaata gtctcaatac ttttttaaat
    10681 atgtattcct ttatatatcc agaagtaacc tattattgca aatgataaag caaataaaat
    10741 atacagtcta aaaaatccca aagaaaaacc tctcttagtt tcaacaaagt atcttgtata
    10801 tgcaactgca aaagatgtaa ttaatgaatt tcctataaca actccaaatc tattatctat
    10861 tccaaatata gataataaca aaggaaaaac tacagtactt acaagagatg aaactattag
    10921 tattaatcca cttttaaaac ttatagttgg aatattataa tctgttcttt gatttttctc
    10981 catttgtttc ttagtatttt tattactttt tgcattttta taattattat ttttctttct
    11041 tttctttttt ttattattag acatgtcatt tacctccaaa aggtttaggt gttatattcc
    11101 ctaaaataac tttattttta gcccctgttg cagatattac attagaaaaa gaattattta
    11161 atgtttcatt gcccaagatt acaaatgcca aagcctcctt tgcatcagaa gaatacccat
    11221 attcatcttg agttaatact tcaacctcta ataataactc ttttatatat cctattaaag
    11281 ttaaattatg agctcctcca cctccaatta ataaagtatc tacattaacc tttggaagta
    11341 tgaagttttt ataattataa tatatagctt ttgccgtaaa catagttact gttgctatca
    11401 aatcatattt atctacatgt ttatatttgt taagcatgtt atccaaataa acttgtccaa
    11461 atacctctct tccagtgctt ttaggtggcg cttgactaag atacttatga ctcatcaaat
    11521 cttttaacat ttcttcatta attttacctt ttgatgcaaa gtatccattt ttatcatact
    11581 ttctattaaa taatctttga catactccat ctattatcat atttcctggt cctgtatcaa
    11641 atgcaaatac atcatctatg ttacatgatt taggtattat agtaatattt cctattccac
    11701 caatattttg aagtgctaaa tttttattgt tatcactata taataaaaat tcactatatg
    11761 gtactaatgg agcaccttct ccacctgctg ctatatccat tactctaaaa ttagatatta
    11821 cctttgtatt tgtttcatat gctataactg ctggttcacc tatttgaagc gttgaagata
    11881 caaaattatt ataacttctt ggtatatgaa agattgtttg accatgagat gcgataaaat
    11941 ccaaatttgc tatatgaaag tttgctttat gacacactga ttttacagct ttactaaata
    12001 aatatccaag cttaaaattt aagctacaga ttaaatcaac actcgattcc tcttctatac
    12061 agcactttct tatttcgtct tttatatctt taggaatttc tagagttata aattctattt
    12121 gcttaacttt agtatttcta ccattcccac taatttcagc tagtactgca tcaactccat
    12181 ctaaggaagt tcctgacatt aaacctacac tatacatagt tatcatcctt ttaagtttat
    12241 agttattcct ccccaaatca aggtattaag tttaataatt ttagaattaa tcttatttca
    12301 tctttaatta cgataattta ctcttatgaa attaattgag ttaatcttat acacgttttt
    12361 taccttagct aaatcttaca ttaatttatt ttcagtgtca atgtaatact atgtaatttt
    12421 ttttcacaaa caaaacatat tctctctcca aaaaccatat taaaatagtt actacaaata
    12481 aaaaacatac caggtacata tctgatatgt ttttcatata taataattat tattttataa
    12541 tatagataga acatcattta aggcactaat taaatcttca ctattttcaa gaactaaatt
    12601 tattcttatt gtatatgtcg atttgtatat gatgatagat tttatagtct aataacctaa
    12661 ctatctaggc ttaaataatc tgatatatca taaaaataga tattaaaaat tgtatatgaa
    12721 ccattttccg aacatacctc aatctcatgc cctaaactat ctatcatttt ctttacaaaa
    12781 tacatcccca ttccagtaga cttatacaat tgatttcttc catttttccc cgtaaatccc
    12841 ttgtcaaaaa ccctagatat atcttcctca tttataccta taccaaaatc ttttatagat
    12901 aataccactc cattttctaa cttttttgaa ttaaattcaa tcttaccaac ttcctttgaa
    12961 tactttatag cattattaat aatttggtct aaaacatatg acatgcattt agaatctgtg
    13021 taaacatcat tttctaattc attcaaacta acttctacat tattttttat aagcaaaaat
    13081 gaattatttt ttatagacat ttttaccaat ttttctaaat taaatttatt tataaataca
    13141 tcttcaaaca tagtgttgct tctactacta tacaaaatag agtttactaa aaaatttatc
    13201 tttgcaacct gattcttaat atccaaacta tcttctatgc tacttaacct atctgtaatt
    13261 atacttaaag aagatattgg aatttttatt tcatgaaccc atttagctat gtactcatcc
    13321 aattctttta atgattgttt ataagtctct atgtcactat tattttttat ttttaagttt
    13381 tctataatat cttttattag attttcttca aaagattgtc catcaactaa a
//
LOCUS       DAGSHT010000095        13149 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGSHT010000095 DAGSHT010000000
VERSION     DAGSHT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.097"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            85..2313
                     /locus_tag="MJB72_002285"
     CDS             85..2313
                     /locus_tag="MJB72_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBY8263385.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2551..2850)
                     /locus_tag="MJB72_002286"
     CDS             complement(2551..2850)
                     /locus_tag="MJB72_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBY8263386.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3056..6097
                     /locus_tag="MJB72_002287"
     CDS             3056..6097
                     /locus_tag="MJB72_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBY8263387.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKETWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVNEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6258..7532)
                     /locus_tag="MJB72_002288"
     CDS             complement(6258..7532)
                     /locus_tag="MJB72_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY8263388.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKILAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(7753..10314)
                     /locus_tag="MJB72_002289"
     CDS             complement(7753..10314)
                     /locus_tag="MJB72_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263389.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLFTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            complement(10315..10992)
                     /locus_tag="MJB72_002290"
     CDS             complement(10315..10992)
                     /locus_tag="MJB72_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263390.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            complement(11072..12316)
                     /locus_tag="MJB72_002291"
     CDS             complement(11072..12316)
                     /locus_tag="MJB72_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263391.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKSQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            complement(12303..13028)
                     /locus_tag="MJB72_002292"
     CDS             complement(12303..13028)
                     /locus_tag="MJB72_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263392.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
BASE COUNT         4604 a         2007 c         1595 g         4943 t
ORIGIN      
        1 taactttact atttgtgtaa aaagtaatat aatttaaaat tgattataaa tctaactttt
       61 ttctttatca aaatatttta ggaggtgaga catttgaaaa aaactaatag tttaaaagaa
      121 attttaaact ggaataatat caagaattta agaccttcaa agggtttaaa aaatatatta
      181 gaaattttta aaagtgacct aaaagatata cgtggcaatt atgccgtact tgtaatagtt
      241 ttaggtcttg cttttttacc atctctgtat gcttggttca atataaaggc atcttgggac
      301 ccttatggaa gcactgctaa tatgcttgta gcagtaacaa atgaagataa aggaaatgaa
      361 atagcaggac aaaagattaa tgtaggagac caattaattg aacaactcaa agataacaag
      421 agtttaggtt ggcagtttgt agatgaaaaa actgctcttg aaggtgttaa aaaaggtact
      481 tattatgcat cacttaagat tccaaaaaat tttacgaaag atttgacatc tttaattaca
      541 gatgatgtta aaaaagggga aattatctat actgttaatg aaaaaataaa tgcagttgca
      601 ccaaagatta cagataaagg tgcttctaca atacaactac aggttaatca gactatagta
      661 aaaacagtta gtgaaatcgt tcttggtata tcaaacggtg ttggtgcagg aattgaacac
      721 agtcttccaa aattgacaaa agttgaaaat tcccttattg accttcaaaa gagatttgac
      781 catataaata agactgttga tttagcttct gatgctactt caaaattatc tgatacagtt
      841 aaagatatta aatctgattt acctacaatt aaaaaaactc tgaatgatac aaaactatta
      901 tcaagtgact taaaaaaatt cttagaggat acaaatgata atttagacga actttctcct
      961 cttattaagt ctgacttaaa tttaatggtt gacctttcat cttctgcttc ttctcttaca
     1021 ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg tacctaagtt gatagataat
     1081 ttatcagaaa aactttctaa tttgcaatct ttaaatgata ctttggtgga ttttttaact
     1141 aaattaaacc aacttacatc aaataataga ttggatgatg taatagataa tcttgaagac
     1201 tctagcaata aaatagattc atctatatca acattaaatg atattaaaaa caaagttata
     1261 tctggacaac aaccatctat aagtgctttg aataatgttt taagtctatc aaatggtatt
     1321 ggaagaatta atttaaatat tttaaataat tttgactcaa aaatatcaaa acctataaat
     1381 agtatatttg caaacagtat aaaagtagca aatgatataa ttactgtttt agataaggca
     1441 gaagcaaaac ttccaaaggt tgaagaaata cttactactt ctctaaaact ttcaggaaat
     1501 gcacaggaaa gtatatcatt gataagagaa agacttcctt tagcaaaagg aatgttggat
     1561 gatttgattg atactttaag caaaattagt aatggtgaag atatgaaaaa attagtcagc
     1621 ttgcttgaaa gtgatatatt gacaaagtct aatttcttaa aagaacctgt tgaggtgaaa
     1681 acagagaaat tatatccaat tgaaaattat ggttctgcaa tgacaccatt ttatagtgtt
     1741 ctttctacat gggttggtat attacttatt tcagcactat tgtctacatc tgtacatgga
     1801 aactataaac cttatgaaat ttactttgga agaggcctta cttttttaag tatagctctt
     1861 gtccaaggtt ttataatagg aactggagat atattattgt taggagtaac agcaaaacat
     1921 ccaattttat tgatagttct gcttatgttt actagtatgg tatttaactt tatagtgtat
     1981 tctttagttt ctgtatttgg aaatgtagga aaagcaattg caatagtact attagtcgtt
     2041 cagattggtt cttctggtgg tacattccct atacaagtta cacctacatt cttccaaaga
     2101 ataaacccta taatgccatt tacctatgca ataggtgcta caagggaagc agttggtggt
     2161 atatatgctc caaatctaac taaagatata ctagttctat tactatttat gattctgtct
     2221 atttgtttaa atgtacttct aaaaggacct ataaatagag ttataaaacc atttaatgat
     2281 aaattcggcg atagtaattt aactggtcat taaaaaatta attctgtctt taaatatatt
     2341 tttttaataa tattctaagt tcatattaaa aggtagtagc taaaaagcta ctaccttttt
     2401 ccaacttaaa aactaatatt tactacaaaa actgtcatag aaaaaaacga aaaaattcgt
     2461 tctttcctaa atttatctta taaatattta gatatattcc tacttaataa gcagagctat
     2521 acatagtatt taatgtataa cttacattat ttaactcata taatcaatta ttcctgaatt
     2581 taaattatat aaatgctcaa aaccttcttc agctaacaaa ttacatgcag taacacttct
     2641 atgaccactt ctgcaataaa ctactacttt cttatcttta taatcgtaaa tttcatctat
     2701 attattgatt aactcttgta aagatatatt tatagcattt tctattttat attccttaaa
     2761 ttcatcttta gttcttacat ctaataataa aacatccttt tcatttttca tcatttcttt
     2821 aagttgcttc ttatttatat tcttatacat tgtctaaaat tcctccattt tatttatata
     2881 ttacctatac cctttattct ttatacaaca acaaatatta tttttatatt tttctctgtt
     2941 atataaaaag ctatacttat aaaagctttt tttattgata ataatctgtt gattaaaata
     3001 attaagaatg ttatgctaat tataaattat taaaaaggga aagaggtaat gtaatatgtt
     3061 taagcaaagg ctctctaaat tattatctag tacattagta ttgagtatgt tatttactgc
     3121 tgctccaaat attacttttg ctgataatac aaaagataat tccgaaaaat atcaaagttc
     3181 tgatatagaa ttacatgatt atagtaaaaa cgcggaatct tacacaaaaa ctaaagctct
     3241 tgctaaagag aaaatccaaa ctttgctatc aaagtatggt gctgtaagtg ctcaatatgc
     3301 tttgattgat aatggcaaaa ttgagatttc tggtaatggt ggagtttaca gcaaacagga
     3361 taacaaaaac ttaaataagg ataatatgta tagtatcgca tctatcagta aaatgtttac
     3421 tacaacagct gttatgaagt tggttgatga tggaaaatta aatttagata ctcctgttgt
     3481 aaagtatata cctgaattta aaatggctga tgatagatac aaggaaatca ctccaagaat
     3541 gttattaaat cactcatcag ggttgatggg aagtagtttt aaaaacacta ttttattagc
     3601 agacaatgat tcttatggtc atgataattt cttaaaagaa ttacaaaaac aaagattaaa
     3661 agctaaacca ggagcttttt ctgtttattg taatgatgga tttactttag ctgaaatttt
     3721 agtagaaaga gtatctggta tgtcatttac taattttctt gataaataca taaataatcc
     3781 acttaactta caaaatacaa aaactccaga aaatagtttt gatagttcaa aactagcaaa
     3841 agcatacgta ccttattggg aagatgcagt tccgcaagat aaccttaatg ctataggtgc
     3901 aggtggttta tattcaagtg cagaaaatct atgtacattt gcacaaacat ttatgaaaaa
     3961 ttcaaatggt attctttctc ctgcttcagt aaaagctatg gaaaacaaag aatatttaaa
     4021 tggtctatgg cctgaaggag aagattctat acttggatat ggtttaggtt gggattgtgt
     4081 caacacttat ccatttaatc agtataattt aaaagctctt acaaaaggtg gagactcttt
     4141 attattccac agtaatctta tagttcttcc agatgaaaat atggcagttg cagttctttc
     4201 ttctggtgga agtagtcaac ttaacgaaat aattggacaa gaaatcttac tatctgcatt
     4261 aaaagaaaaa ggtaaaatta aagaaataaa acctgataaa acatttagta aaccacaaca
     4321 agttaaaatg cctagctctc taaaagaaaa tagtggtctt tatgcttctt caaatatgat
     4381 aaaagtagat gttaatgata atggtacttt aacagtatct tctccttata tagaaaatgg
     4441 acctgaagat aaatacgtat acataggtca agatagattt gtatctgaaa aaggtaacag
     4501 ctgtctaaaa tttgtaaaag aaaaaaacaa tatcacatat ttaaatatga gctcttatga
     4561 tgatgtccct ggattaggtc agacagcaag tttatactat gtagctcaaa agatagatga
     4621 caacaatata tcaaatagtg taaaagaaac atggaaaaaa agaaatggta aagattacta
     4681 tctagtagat gaaaaatata cttctcaaag ctatatgttt ggttctgtga aggcaactct
     4741 tgctctttct gatgaaactc caggttatat agttaacact aaaataatgg atgagaataa
     4801 ctcaaatgct tttatagaaa tacctggtgt aataggtcgt gatttatccg acattaaact
     4861 tcataaagaa aatggtacag agtatttatc ttttggaaca ctaacgtatg ttaatgaaga
     4921 ttctataact aatttgccag cagaaaaatc ttttacttgt gaactagaat ccaatggtta
     4981 tgcaaaatgg tataagattg gtgacgatat cgcaaataaa aaaatagaag ttaatcttcc
     5041 tcaaaattca tcttttgctg tttatgatga taaaggtgtt cctgtaaact attctttagt
     5101 tacaaagaac aatagagtta gattgccaaa aggaggcgta attgttttct taggaagtcc
     5161 aaatgctaga tttgaggtta catatcaaga tgaagtaaat gcaagtgcac ttacaggaac
     5221 tgatagatat gaaacttcta ttaagataag tcaagcaggt tgggaaaatg ctgaaaatgc
     5281 agttttaata aacgattcag caattgcaga tgctttggct gcaacaccat ttgcatacaa
     5341 gaaaaacgct cctatattat tgactggaag ctctcaaata aatgaaaaaa ctttggctga
     5401 attaaagaga ttaaaagtta aaaatgtata tgtagttggt ggagaggctt ctataaatga
     5461 aaaatcttta gatactataa aatcaaacaa tatctctgta tctagaattt caggaagtga
     5521 tagatatcaa acttctatga acattgctaa agaattgaac aatattagca atatatcaaa
     5581 gatttcagta gttaatggcg aaaaaggatt agccgatgca gtaagcattg gagctgtatc
     5641 agctcaaaat gatatgccta taatacttac taatgaaaat agtaatataa ctgaaataaa
     5701 taacttgttt aaaaacaaaa agattgataa atcttatgta atcggtggag aatatacagt
     5761 ttctaaaaat atagaaagta aacttcaaaa tccacagaga atatcaggta gcactagaaa
     5821 tgaaactaat gcaaaagtaa taaaagaatt ttacaaggat tctaagatag acaatttata
     5881 tgttgctaaa aatggaatga ataaacaaga tgatttaata gacggattat ctgttggagt
     5941 tttggctgga aaaactaaat ctcctgtaat gctagtagga aactcactag attataatca
     6001 aaaagaacta tttaaaacta tgagatttaa atctgtaaca caaattggtg gaaatggaaa
     6061 tgaaaactca tttaagcaaa taaaagaaat agcttaacat aactaaaaaa ggctgtattc
     6121 aagtaatatt aattgaatat agcctttttt tatttaagtt ataagtttta tatgtgtaaa
     6181 aattaaattt aaatattaaa tgataagttt attaagatat agataagtct aaaactttgc
     6241 cttttttaag ttagtttcta tttatcattc atacttaaca atttatctaa atatactgac
     6301 tttaattgtt tactggctaa aatcttttta gctggttcaa gctttaatat agctgacagt
     6361 atagctccca tagctctatg actagccaga gcattattat caaaaattaa attttgtaac
     6421 tgcccttttt caattgccat aagaactgct ctatgtactg aatttgctgg agttattatt
     6481 ttttcatctc tctttaataa cataatgcta tttttatggc aatttctaac acaaactcca
     6541 caacctaaac atctatcctc atcaattttt atatattctt taccatcttc cttaaccttg
     6601 cttatcgcat ctattggaca tgcagttata catttaccac actttacaca attttcatgg
     6661 tttatatttg gtataaaact tgttgtctgt actggatgaa gatttccaaa tcttttagct
     6721 gctaccattg cttcacagca acatccacag caattacata taaacgtaac ccctttccta
     6781 acattttctc cacattgaac taagttatgt tcatatgctt gatgaagtag ttctttacat
     6841 tctattttat ccacacgtct tgcaaattta ttatttatta aagaatttgc tacattgtca
     6901 aacgtcatac atatatccat aggagcatca caagcttttc caacatgttg cattctatgt
     6961 ctacagtaac acatacttat acctatatga gttgattcat caattatatg agtagccctt
     7021 tcataatcta aaatactaac ctcattatca ttagttaaaa cttcctcttg aacgtatact
     7081 cttccaagct tcgtttctgt tgaataaaat aaatctttga taaaatcttc ttctacattc
     7141 atatattgat agtaaagctc tgccaaaatt ttttggtcta tatcgtgtct cgttctcatc
     7201 atggcaaact caaaaaaacc agccattgga ggaggcatta tatatttctt ccctttatta
     7261 tcttctatat ctagtatcag agccttacta gcaagtttat ccaatactct atatgcttca
     7321 ctttcactta cactccaaat tttagctgct gtttttactc tgaaaggctt tattggaagc
     7381 tgagcaacta acttcgcctc ttgttctgta tatagtacat ttagtatttt gtataatgta
     7441 tcagatggtg gagcaccttg tggaaacttg tttattcttt cctctaaact tttatacata
     7501 ttcttcgcag ttatatgaga aatagtaatc accttctttt tcttttgttt tccaataaaa
     7561 tcaaatattc tttcttatta tatattctct tactttatat aataccaatt ctttctaaat
     7621 agttaaatac ctctagtaat ttattattag atttcttata taaataaaaa atcatttaag
     7681 tgatataata atattttata ccacttaaat gattttaata atattttata tagttaagaa
     7741 ttatttttat atctatatac tttctcttct tatattatct attatatttt ctttatttaa
     7801 tttactcatt ggtatatacc ctgctataaa agttattata aaaacactta ctatacagat
     7861 aactatggct gtccacggta taatcggaga aaattcaatt acacctgaaa taagtttatt
     7921 cataattaga attataccaa tactaattgg tattccaaac aacaatgaca gtataccata
     7981 tataaaactt tctaaatata tcattttcct aaatccttta ggagtaaccc ctatagattg
     8041 tattactgca agttctcttt ttcttagatt tatacttgta cttactgtat ttagaatatt
     8101 tgtaacactc actaaagata tcactactat aaaaccatat acaaatattt taataacctt
     8161 taaatcttgt ttttcacttt cattgcttgc actttcatca taaaagttaa aattattttt
     8221 atttgccaaa gcttctattt ccttcttagt cttaatgctt cctcctgaat caataaatat
     8281 aagactttta cttaatttta aatttaaatt tttagcaact tcatcataag ttataaattg
     8341 taaccccata ttttgataac caagacctag acgttcttca tcagttatag caagaatctt
     8401 gacctttact tttttactta aggcattttc atcaagatat tcacaattta ctgtatctcc
     8461 tactttatag ttggtaagtt ctactacacc tttcttccca ttttcttcaa atgcactttt
     8521 tctaactaat ataacaccat tttcttcaat tgctctctct ttattaaaag agccctgtac
     8581 taagttattt attttgtttt ttactgcaaa atctcctgga aaaattaatt cattattact
     8641 gaaatcatat tcataactat ctccaatctt atatttttga taatatttcc tgattaaatc
     8701 ttccttggat ttatttattt tattttcact tactcttata gacacatatt gtcctgttgc
     8761 caattcaaag ttttttatcc catcaatatc ttctagttca cttatagtat cctctacaga
     8821 cctagctgtg ttctttgcag ttaaatacaa atcatatttt ctttgagaat ttctaatttc
     8881 ttcacctctt agtaaaagta atgtaaagcc actaaaagat ataaatatta ttatacttac
     8941 cattaaagaa aaaagagtta ttataaattt ttttctattt ctcctaatat ttttatatgc
     9001 aagtactcct tcagttttaa aaatcatttt aataagtctt gagcttttga ttttgcccac
     9061 tttatactct ccagtatttt taatcacatt aaggggagaa atattagaag ccgatattgc
     9121 aggtagtaga attgatataa agattgtaaa caaaactatt attatactaa caataattat
     9181 tattggatta tacactatct gcaaactcat cttagtaaca accgattcag taaaatactt
     9241 atttataatt ttaaacaata agtctatagc tactgttcca gatattagtc ctattggtat
     9301 accaattaaa ctaattataa taccttctat aaaaactaat cttttaattt gactagatgt
     9361 agcacctata gaatttaata tcccaaactg tttttttcgt tcatttatag aaatactaaa
     9421 ggaattgtac actgtagcaa ttgtacatac gattactaaa atagttactg ctataattat
     9481 agactttata gagctattta tatttgaata cttactagca ccttgtaacc tcaataaatg
     9541 ttcattgtat tttatcatat cagaaactgc ttctttatct ttatttgaat atatattttt
     9601 acttatttta ttacttatct tatacacatc tttaggattt tttactgcta ctgatacatt
     9661 aattgtatca ttgtaattat tcataggatt aaagtaggct atagctgttg caattccatt
     9721 ataacgttca aacccaggtt tttcaattat accaacaact ttaaaagttc tatctttttc
     9781 atttgctata taatcacctt catgaaatgt catactatca atctttttct tgcttgagtc
     9841 aaacatatcc cctactttta aagttatctt atctcctatt tttaacttat catctataag
     9901 attaataata ctttcactta aaacaatttc tccttcctta gctggaaatc taccttcttt
     9961 tatagaaact ttatatccat ttattgcatt tttatcatat tgctttatac ttaatatagc
    10021 attttcacta ttttctaatt tagaatatcc aagttgtttg gtaaatgcat gtttttctat
    10081 ttcagctgat ttcgttacat attccacatt ttttctattt acatcataaa atgtaacatg
    10141 gaaacttcca tcattcttta tagtttctct catttgataa tccataaaac tttcaaatat
    10201 atttcctata ccacaaatta aagctgttga aagtattata ccgattatgg ttacaatggt
    10261 tcttctttta ttttgtttta agtatctaat ggttagtgat gtatataaat tcatctacat
    10321 cacctcatca ctttttatga ttccatcttc tatgcttata attctatcag cttgaagtgc
    10381 catatttttg tcatgagtta tcattattag tgtctgattg tattttttta ctgacaattt
    10441 taacaattct aatatttcct ttgaattttt actatctaaa ttacctgttg gctcatctgc
    10501 taatattatt gaaggtctat taattaatgc acgtcctata gatgttctct gttgttgacc
    10561 tcctgataat tggtttggaa ggtgcttttc tctatcaatt agacctaatg ttttcattaa
    10621 atcatcaaaa tattctctat caatatctct attgtcaagc tctgctggaa gcaatatgtt
    10681 ttccttcaca tttaagactg gaattaagtt gtaaaattga tatataagac ctatatttct
    10741 tcttctaaat atcgccaaat ccttagtttt taaattatat atatcaacat catttatata
    10801 aaccttacca ctagttggtc tgtcaactcc tcctaataaa tgtaataagg tacttttacc
    10861 acttccactt ggacctgtta ttgctataaa cgttcctttt tcaactgata aacttacatt
    10921 ttttatagca tcaacttttg tttcattttt accataactt ttagttagat tttctactct
    10981 taaaatttcc ataactacct ccaatattta tattctatac ttcatatatt aggttttggc
    11041 tattactata aagtgaatta aatattacag attagtcact atcatacatt ttatgaaata
    11101 ttatatgaaa ttctgtaccc ttaccttttt cgctgtttac atatatatcg ccattttggc
    11161 tttctattat agattttgac attgcaagac ctattcctac actatcttct tttgaactac
    11221 ttcttcctct atagaatcgt ttaaatacat gtggtatatc ttttttatct ataccttcac
    11281 cctcatcttt tattattagt tcagaaaata atggattttc tttataggtt ataattatat
    11341 ttccaaattc aggagtatgt tctacacaat ttttgattat attcactaat gcttccacgg
    11401 accaatcaat atcaccaata taagatacat tatcatttcc ctctatagtc agtttttgat
    11461 ttttaatctc cataggtatt ttcatagata gcattgctct atgtatgagt tcactaaatt
    11521 ttaccttatc ttttttaaaa tttatcactt ttgcttctac ctttgataat ttaagcatgc
    11581 ttttaatcaa ccaatccatt ctatttaatt gactttttat cttatttaaa aaatcgattt
    11641 ttacttcgta tggtacatca ttatataata aatcattcaa cattattaaa gaagtcatag
    11701 gagtttttaa ttgatgtgat atatcggaaa ttgtattgtt taaaaatatc ttttctcttt
    11761 ttaaaagttc aaccttttca ttaagaattg tcgtcatctt taagagttca gtttttagaa
    11821 gacctatctg cccttcttga ttcttgtttt tcatatcaaa tggcttaccc tctgaactat
    11881 agtaaacata cttagtcata tctgataaat cattgtaaat tgacgtaaaa tatctaacaa
    11941 ccaacacaaa tatcaaaata aatattatac atactaatat tatatttatt ttgatagtct
    12001 ctaaaactaa tttagaaata ataggctcag agtttatctt aatagattta tcataattat
    12061 acttaattaa tattttctga ccataatcta tgttctccat agacttccct tgagttatta
    12121 tgtctactat ctgactttct aagtttggat acttgcttga taaagttcct attatagctt
    12181 gattatttct tacaaccatc tctttagtta aagacacgtt gattatgcta aatccaatag
    12241 atattatgat aacaataaac atcaaagtaa catacttact taaaaacttc ttcacttctg
    12301 gattatatag aaacactatt ttcacttcct atccatttat aaccaatccc ccttacagtt
    12361 tctatgtaga ttggttcaca taaatcatca cctatttttt ctctaagcct ttttatataa
    12421 acagtgagag tattgtcgtt tacaaaatca taagttacat cccaaagttt ttccaatatt
    12481 tgagttctac ttaaaacagt atttttattc tgtataagaa ttaaaagaag tttatattca
    12541 actgatgtca gaaatatttc tttatcattt ttatatactc tagcttctaa tgtatatata
    12601 cttaaatctc caaatttaag tattttctta ttttcttctg aagtattacc ttttcttctt
    12661 aaaacagctt ttattctgga tattaattct ctaattctaa aaggttttgt tatataatca
    12721 tctccaccta tatcgagacc cattacaata tttacttctt catcacaagc agtcaagaat
    12781 attataggaa cctgagagaa tcctcttata tattgacaaa gctcataacc agttccatct
    12841 ggcaatgtaa catccaacag tatcatgcta tattcattag aagaaatcat ttcttttcca
    12901 ctagataaat cctttgatat atctatacta aatccttctt gttctaaagc atactttatt
    12961 ccaaaagata tagtactatc atcttcaact atcaacaact ttaacataac tatttccttt
    13021 cttttcattt taattttaaa ctatctgctt aatttcatac tatctatcta tacaagtttt
    13081 attatacaca attaccctgg atactaaaat atttctaaaa ttacaaaata gtcacaaaga
    13141 actattaaa
//
LOCUS       DAGSHT010000096        13080 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGSHT010000096 DAGSHT010000000
VERSION     DAGSHT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.098"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            95..1132
                     /locus_tag="MJB72_002293"
     CDS             95..1132
                     /locus_tag="MJB72_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBY8263393.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            1213..1578
                     /locus_tag="MJB72_002294"
     CDS             1213..1578
                     /locus_tag="MJB72_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263394.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            1583..1900
                     /locus_tag="MJB72_002295"
     CDS             1583..1900
                     /locus_tag="MJB72_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263395.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            1948..3552
                     /locus_tag="MJB72_002296"
     CDS             1948..3552
                     /locus_tag="MJB72_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263396.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            3676..5304
                     /locus_tag="MJB72_002297"
     CDS             3676..5304
                     /locus_tag="MJB72_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBY8263397.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            5552..6901
                     /locus_tag="MJB72_002298"
     CDS             5552..6901
                     /locus_tag="MJB72_002298"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBY8263398.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            7057..8136
                     /locus_tag="MJB72_002299"
     CDS             7057..8136
                     /locus_tag="MJB72_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBY8263399.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            8481..9389
                     /locus_tag="MJB72_002300"
     CDS             8481..9389
                     /locus_tag="MJB72_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBY8263400.1"
                     /translation="MNPLVINLQKCSIHDGAGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            9394..11763
                     /locus_tag="MJB72_002301"
     CDS             9394..11763
                     /locus_tag="MJB72_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBY8263401.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            12088..12891
                     /gene="proC"
                     /locus_tag="MJB72_002302"
     CDS             12088..12891
                     /gene="proC"
                     /locus_tag="MJB72_002302"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBY8263402.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVLIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5268 a         1390 c         2454 g         3968 t
ORIGIN      
        1 atagaatatt tacttatact atagattgat taataaaaaa atggtataat aacaaattgt
       61 actttaattt aataataagg gagtggaagt catattgtta gggagaaatg agctatgccc
      121 ttgtggaagt ggcaaaaaat acaagaaatg ttgtttaaat aaagatattg tttcagaaag
      181 agcatgcaga aaaactgaac tttcacaaaa acaatataca gatttatatt caaagttata
      241 tgattattca agacaggata agtttaaaga agaatgtgaa aaagctaaag aaatgtttta
      301 tataatgcaa aatgatagtg taaatgaaaa atttgaaaga ttttttaata cttatttaat
      361 acaagaccac ataatggaaa ataaaaaggt tatgacagtt ggttttcttg aagaaaatgg
      421 tgcaaaccta agtcaatcag aagtatcaat attaaaaagt atgtttgaat catacgtaag
      481 tatttatgag gtaaaagaag tttctgctgg aaaaatagta ttaaaggact gtttaagtgg
      541 aaatgaactt tgtacagaag atgtaaaact tctgagaagt tttaaaatcg gaagttgtat
      601 gatagctaga atagttgata ttgaaggaac taacatattg atagatataa ctataagtat
      661 atcaaatgag gttaaagata taattttaaa ggatattatg aatctattca atcaatatca
      721 agatgtatac aaagatatga agtcgttctt aatttatcat acacatatac tatacaagta
      781 tatacaacag cttttagacc caagtatagc tgaatattta aagagagaaa gagataaaaa
      841 cgatacaaaa gttaattcag aaaatgaatt agctgaagaa gaatgtaaag ttttagatac
      901 tcttaaagaa aatgtagaaa aagaaaatat agatgcatgt atagagtttt ggaatgaata
      961 taagaaaaat catgaaaata taaaaggagc tgaaaatggt tgggcagctg ctgttgaata
     1021 ctatgttaaa aaagaagcag gacaacctat aactcaagtt caaatatcaa agaaatacga
     1081 tataagctct agtacactag gaaagagata caaggattta aaaatatctt aattaattat
     1141 aaaaagcttt aagtcataga taaagaatct atagtataat atatgtataa tgtttagaaa
     1201 gaggtattaa taatgagtct tagtttagga aatgcaattg ctgtatcaac gaactttata
     1261 aaatctaacc cagctgtacc tgtgtacaac ggagaagaaa tatatgcagt tatttatcat
     1321 atagaaatag gatataataa tggtgtatca cctgatacca aacaatatat aataatagac
     1381 tataaagagt ttgatttatc tatggataaa aaaaatgaac taataaatat tgctaaagag
     1441 tactgtaaat ctaaaggtat tgtagaaaac gaaaaccttg aaataaacct tttagacaat
     1501 gaagaagcag agaacttctt agaaaaggtg tttaataata tgaaggttat aagaggtctt
     1561 ataactaaaa aaggataaat aaatggacta tataggaata gaaaatataa caccttatga
     1621 aaatacatat gaatttagtg tatatgaata tgatgatgaa atcaccttag gtagtgaaaa
     1681 gttatatgta tgtgaattaa gggttgtatt gattaaagtt aattctctgt atgttgaaag
     1741 attgcataaa tcagttgaag caatggtctt agtaaaaaac ttgaaaaaag atttagataa
     1801 aacacttgtt gtaaacaaaa taaagaattt tgtgctagat gagatttggg tagaaaatct
     1861 agtaaaagag aatatagaag ttatatttgt agaaagctag gaataaccct agctttttta
     1921 tattacaatc taagagagga aaacaatatg ttaagagtat ctaatgtaaa acttggaatt
     1981 gataaagata tatctttatt aaaagatgtt atacttaaga agctaaaaat aaaagaaaaa
     2041 aacttagtca aatatagtat atataaagaa tctatagatg ctagaaaaaa aggaaaaatg
     2101 gaatttgttt attctgtaga tgtagaagtt aaagatgaaa ataaaatact taactcaaaa
     2161 ataaaagatg taacaaaaat aaaggaaatc agatacgtaa atgtacctgt gggtgataaa
     2221 aaattaaaga ataatccttt ggtaattggt agtggaccag ctggtttgtt tgcaggtctg
     2281 cttttagctc aaatgggtta tgaaccaatc atacttgaaa gaggtcttga tgtagacagt
     2341 agaaccaaag atataagtga tttttggaca actagaaaat taaaaaataa ttcaaacgtt
     2401 caatttggag aaggtggagc aggaactttt tcggatggta aacttacaac tagaataaaa
     2461 gatatacgat gtagaaaagt attagaagag ttagttcagt ttggagcacc agatgaaata
     2521 ttatactcac ataagccaca tgttggaaca gatatattaa aaaatgtagt taaaaatata
     2581 agaaatgaaa taataagttt aggtggtcaa gttagattta attcaaaagt aacagatata
     2641 gttgtatcaa atgattctat acaatctgta ataataaatg atactgaaaa aatagataca
     2701 gatacaatta tattagctgt tggacatagt gcaagagata cttatgaaat gttgtattca
     2761 agaggtataa aaatagttca aaaacctttt gcaataggag ctagagttga acatccacaa
     2821 gatttaataa ataaagcaca gtacaaggat ttttataatc atcctaggtt aggagctgca
     2881 gattatagac ttatagagca tgtttcaaat ggaagaacag catatacttt ttgtatgtgt
     2941 ccaggaggta gtgtcatagc atctgcatca aatgattttg aaatagttac aaatggtatg
     3001 agtgaacatg caagagataa aataaatgca aatagtgcat ttttagtaaa tgtaacgcca
     3061 gaagatttta agagtgataa tccactagca ggtgttcatt tccagcagaa gtatgaaaaa
     3121 ttagcttttg aattaggtgg aaaaaattat aatgcaccag ttcagcttgt tggagatttt
     3181 ataaacaata agacttcttt gaatttagga agtgttgagc catcatatag accaggatat
     3241 acacttacaa atttaagtga atgtttgcct agcttcgtaa cagagactat gagagaagcg
     3301 tttatatctc ttgataaaaa attgaatggg tttgcaatgt atgatgctgt tttgacggga
     3361 gtagaaacta ggtcatcatc accaataaga atagtaagag atgaaaatac tttagaatca
     3421 gtgagtatta agaatctata tccatgtggt gaaggtgctg gatatgcagg tggtatagtt
     3481 acagcagcag ttgatggtat aaaatgtgca gaaaaaataa tacaaaaata caccaataat
     3541 acaaaaattt aacataaact taatgatttt ttaatattta acatgaattt aacattatca
     3601 ctcctataat ttgataaaat acaataggtt ataaaatttt tagatacagt aatctcttta
     3661 caaggaggaa ataatttgaa cattataata agtctaatgg gtggattagg tctgttctta
     3721 tatggtatga accttatggg agaaggtctt caaaaatctg ctgggacaaa gctaaaaaag
     3781 ataataaaac tacttactag taacctattt atgggagttt tggtaggtac aggagttaca
     3841 gcagtaatac aaagttcaag tgctactact gtaatggttg ttggtttcgt aaatgcagga
     3901 ataatgactt taaaacaggc aataggagtt ataatgggtg ctaatatagg gactactgtt
     3961 acagcccaat tggtatcttt cgacttaact ggtatggccc cagtagcatt aggtgtaggt
     4021 attatacttt atttatttgg aaataagcct agaataaaaa atatagccga aatacttata
     4081 ggttttggta tattatttac aggtatggat tttatgaaga tggcagtgga gccactaaga
     4141 gactatcaag gatttactga cttacttgtt acatttggta gatatccact acttggatta
     4201 ttattgggat ttgggattac agcaataata caaagttcaa gtgcatctat gggtatgttg
     4261 gttgcactgg cagcagaagg tctagtgcca ttatcagcag cactgccaat tctttatggc
     4321 caaaatattg gtacatgtgt aacttcactg ttatcaagta ttggtgctaa caaaaatgca
     4381 cgaagagcag ctatgatgca tctaatcttc aatgttttag gaacggtgat attcttaata
     4441 ttcttgaata aaccagtagt aagcatggtt acaagttggg acccaagcaa tgttgcaaga
     4501 caaatagcca atacacatac attgtttaat ataatatctg tattgatact tttaccattt
     4561 acaaatctta ttataaaact agctataaag cttgttcctg atagagcagg tgatatagat
     4621 gaagatgaaa ctaaaactat taaatatata gatgatagaa tgatagaaac tccttctata
     4681 gctcttgcaa atacgataaa agaagcactt cgtatgggtg agaaggcgaa ggaaagccta
     4741 aatgcatcta tggaagcttt agtagaacat tcaactgaaa aaatagataa gacatataga
     4801 cgagagagat taatcaatga tttacagaaa gctattttaa attatttact taaactttca
     4861 aaagcacctt tagatgatga ttcaagagaa gttgtggata ctttgtttaa tacagtcaat
     4921 gatatagaaa gaattggtga ccacgctaaa aatatagctg aattatcgca agttgcaata
     4981 gattcaaata tttctttctc agaagaagga caatctgaac ttgatgttat gtataataga
     5041 gttgtttcag catatactta tgcattagaa tcaatgagaa ctgataatgt tgatttagct
     5101 tgtaaggtta taaaaattga agaacaagtt gatataatgg agaaatcttg tagagctaat
     5161 cacatgtata gattgaacaa taatctatgt agtatagaaa atggagttat ttatttagac
     5221 gtaataagta atttagagag aatatctgac catgctgtca atatagcaca acaagttata
     5281 gcaaagagat taggaaatga ttaaaaataa ttaatttaaa atagaggata aataaaagag
     5341 aaactttaaa tgatacgttt agagtttctt tttttgtaaa tttatttgac taaaattttt
     5401 aaatttatac atagttatag tatttagaag aatcgatttc ctgataataa atgatgagat
     5461 atgtatgatt ttgtggcaaa cttttaaaaa aaacacctat attttgtgtt ataatctctt
     5521 taaaacgaat atgctgaaaa gagggattta aatgggaaaa ataaattttg attatagtaa
     5581 agctacagag tttttttgtc aaaacgaaat agatgtcatg caaccatatg tagatgttgc
     5641 gcacgacatg ttacataata aaactggttt aggaaatact tttttaggat ggatagacct
     5701 tcctaaaaat tatgataaag aagagtttga cagaataaaa aaatctgctg ataagataaa
     5761 atcagactca gatgttcttt tggtaatagg tataggaggc tcttatttag gttcgagagc
     5821 tgctatagat atggtaagtc attcttttag gaatggactt aaaaaagagc aaagaaaggc
     5881 tccagaagta tattttgtag ggcataatat aagctcaaca tatattatgg atttattaga
     5941 tataattgaa ggaaaggata tttctgtaaa tgtaatatct aaatcaggaa ctactacaga
     6001 accagctctg gcttttagaa tatttaaaga ttacttagag aaaaagtatg gtaaggaaga
     6061 agcaagaaag agaatttatg caacaacaga tgcaagtaaa ggtgcactta gacagttagc
     6121 tacagaagaa ggatatgaga cttttgtaat accagatgat gtaggtggtc gtttttctgt
     6181 attgacagca gttgggttac ttcctattgc tgctgctgga cttgatattg atgctatgat
     6241 gaaaggcgca aatgatgcta gagaagcatt tcaaaatcct gacttaaaaa gtaatgatag
     6301 ttatagatat gctgttgcta gaacaatttt acatagaaaa ggaaaagatg tagagttatt
     6361 agtgaactat gaacctcaac tacattatgt aagtgagtgg tggaaacagc tttatggaga
     6421 aagtgaagga aaagaaaata aaggtttatt ccctgcttct gtagatttct caacagattt
     6481 acattctatg ggtcaataca tacaagatgg aaaaaggctt ttatttgaga cagtacttaa
     6541 tgttgaaaac tgtaagagaa atataactat aagttcagag gaagtagact tagatggttt
     6601 aaactattta gcaggtaaga ctgtagattt tgtaaatcat aaagcttttg aaggaactct
     6661 attggctcat acagatggaa aagtacctaa tttagtaata aatatacctc aactagatga
     6721 gtataatttt ggatatttag tttatttctt tgagaaggct tgtggaataa gtggatattt
     6781 attgggagtt aatccatttg accaaccagg agtagaagct tacaagaaaa atatgtttgc
     6841 tttactaggc aaaccaggtt atgaaaaaga aaaggaagag ctagaaaaaa gacttaaata
     6901 attttaaata gttaaggttt aattaagatt aaaataatat aatgaaatca tcaaaaagga
     6961 gtttactcaa aatagatgat ttcatttttt ttatgaaaaa ttgggaaaaa tatattttga
     7021 taatttatat tatattaaga tagggagtgg tatgagatgt caagaagaaa gaaagggata
     7081 agtttagtaa tattagttgc tataataagt tcaatactta gtagttttct aacaataatt
     7141 ttagttaagg acaatttagt aagtaaatct acaggaagtt ctacgccgat agttgtaaat
     7201 gatgatggta agagtcaaaa tatttatcaa gcagttgcag aaaaagctac gccatctgtt
     7261 gtaggtatta caacaactag tgttgatact agtaatatgt ttgcaatacc aactgaaaca
     7321 caaggtgtag gaactggaat catagttgat tcaaatggat atatacttac taattctcat
     7381 gttatatcag atggtcaagc tacaagtgta aatgtattat tcaatgatgg ttcaacaact
     7441 tcaggtaaag tagtatggtt tgaccaacaa cttgatttag ctatagttaa ggttgataaa
     7501 actggactaa caccagcaga atttgctgat agtgacaaag taaaagttgg agatatatca
     7561 atagcaatag gtaatcctct tggattagat tttcaaaaaa cagttactca agggataata
     7621 agtggattag atagaactat acaaacagaa aaaactaata tgactggttt attacaaact
     7681 gatgcaagta taaatgcagg aaatagtgga ggaccattgt taaatcaaaa aggtcaagta
     7741 attggtataa acacagcaaa agcttctcaa gcagaaggat taggatttgc aataccaatc
     7801 aatacagcta agagtatagt tgaagaagta atcaaaaatg gaaaatatga aaaagtaact
     7861 ttaggcataa aaggtacaga tgtatcaaat tatgaagcag caacaggtac aaaattaagt
     7921 actgataaag gtgtttatgt tgcagaagta atatctggtt catcagcaga aaaagctgga
     7981 gtaaaggtag gggatattat aactaaagta ggagatacag atataacagg tatgaatgat
     8041 ttaaataaaa agttgtatac tttctcaaaa ggtgctagta caaaaataac agtaaataga
     8101 ggcggaaaag cagtaacaat aaatgtgaat ttctaaatat gaccaaaaag atagagacta
     8161 catggtaaag tgtagtctct atttttattt aatttttata gtatatacat ctaaattaat
     8221 taaaatgatg tattgtttat agtttattgc tatattttct tataaaaata aatttaataa
     8281 atatttaaat ttaatttaca aaaacaatat aaatatcata ataaaataaa attttttaat
     8341 attactaaaa atatatagac aaaataataa caaatatagt attatgtaat tgtagaaatg
     8401 cttttcatag gtatattcta tgaagatgaa ttgcataaaa atgaaaaaat ggaatggata
     8461 ttatttaggg gtgaaaaatc atgaatccat tagttataaa cttacaaaaa tgtagcatac
     8521 atgatggagc agggataaga agtacagtat tttttaaagg gtgtccactt gaatgtgttt
     8581 ggtgtcataa tccagagagt caaacatata caaaacaagt tttgtataat gaagaaagat
     8641 gttcaaaatg tgaagcatgt ataaatatat gccctcataa ggcaatttac aaaggtgaaa
     8701 caaaaatatg cttagaccaa gataagtgtg agttttgtga aacttgctta gactattgtg
     8761 taaacaatgc aagagagatt gttggtcaag agtattctgt aagagatttg gttaaagaga
     8821 tagagaagga tagaattttc tatgaagaat caggtggtgg agtaactcta tcaggtggag
     8881 aagtaatggc tcaagatatg gacttcattt gtggagtaat aaacatgtgc aaatcaaagg
     8941 gaatccatgt agcaattgat acatgtggct atgcaaagtc agaaaattat gaaagagttg
     9001 ctaaatgtgc agatttgttt ttatatgata ttaagcttat tgatgaagat aagcacataa
     9061 aatttacagg aaaatcaaat gacttaatac tgaaaaatgt taagatttta agtgaattag
     9121 gggctaacat aaatataaga ataccattaa ttgtaggagt aaatgtagat gatgaaaatt
     9181 tagaggttaa aaaaatgata gagtttttaa agcctttgaa tatacaagca gtaagtcttc
     9241 ttccatatca taatattggt aaacacaagt atgataaaat ttataaaaag tatgaaggag
     9301 aagaactaca aagaccatca gaagaaaaat tagaggaaat aaaaagactg tttgaagcaa
     9361 gtaatttcaa tactaagatt ggagggtaat aaaatggcaa gaggaacttt tgagagaact
     9421 aaaaaattaa gagaagaaag tattaatgca gaaccacaca tatctattga aagagcagta
     9481 cttatgacag aagcttataa aaaatatgag ggaagtgtag agattcctgt acttcgtgct
     9541 ttatcattta agcattatat tgagaataga actcttagca taaatgatgg agaattaata
     9601 gtaggtgaaa aaggtgattc tcctaatgga gcaccaacat atccagaaat ttgttgtcat
     9661 acaatggaag atttagaagt aatgcacaat agagatataa taaattttag cgtatcagaa
     9721 gaagctagaa aaatacataa agaagaaata attccttttt ggaaaaagag acaaacaaga
     9781 gataagataa taaatgcaat gacacctgag tggttagcag catatgaagc aggtatgttt
     9841 acagagttta tggaacaaag agctccagga catacagttt gtggagatac aatatataaa
     9901 aaaggatttt tagatttaaa gaaagacata gaagcaagat taaaagagct agattttctt
     9961 aatgatttag atgcatataa taaaaaagca gatttagaag ctatggcaat agcttgtgat
    10021 gcaatggtta tattaggtaa aagatatgct gagaaagcaa gacaaatggc agaagaagaa
    10081 acagatgaag ctaagaaaaa agatttatta ctaattgctg aaacttgtga tgttgtacca
    10141 gctcacaaac cagaaactta tcatcaagct atacaaatgt attggtttgt tcatataggg
    10201 gttactactg aacttaatat atgggatgct tttactccag gaagacttga ccaacatcta
    10261 aatcctttct atgaaagaga tgtagaaaat ggaatactag atagagatag agcacaagaa
    10321 ttacttgaat gtttatgggt taaatttaac aatcagccag caccaccaaa agttggtata
    10381 acattaaaag aaagtagcac atatacagat tttgcaaata taaacactgg tggaataaat
    10441 ccagatggtc aagatggtgt taatgaagtt agttatataa tacttgatgt tatggatgaa
    10501 atgaaattga tacaacctag ttcaaatgtt caaataagta aaaagactcc tcaaaaattc
    10561 ttaaagagag cttgtgaaat atcaagaaaa ggatggggtc aaccagcatt ttataatact
    10621 gaagcaatag ttcaagagct tatggaagct ggaaaaacta tagaagatgc aagacttggt
    10681 ggaaccagtg gttgtgttga aactggatgt tttggtaaag aagcatatgt tctaactgga
    10741 tatatgaaca ttccaaaaat acttgaatta acattaaata atggatatga cccaattagt
    10801 aaaaaacaaa taggtattga aactggagac cctagaaatt tccaatctta tgaggaacta
    10861 tttgaagcat ttaaaaagca attacattat atgattgaca taaaaataga aggaaatgct
    10921 gtgatagaaa atatatgtgc aaaacacatg ccttgtccac ttatgtctac aatagttgat
    10981 gattgtatag aaaaaggcaa ggattaccaa agaggtggag caagatacaa cactagatat
    11041 atacaaggtg ttggtatagg aactataaca gatagtttaa cagcaataaa atataatgta
    11101 tttgataaga aaaaattcga tatggataca ttgcttaaag cacttgatgc taattttgaa
    11161 ggatatgaag ctatacttaa tttagtttca aataagactc ctaaatatgg aaatgatgat
    11221 gattatgctg atgaaataat gcaagagata ttcaatgcat attacaatga ggtaactggt
    11281 agaccaactg tttgtggtgg agaatacaga gtagatatgt taccaacaac ttgtcatata
    11341 tactttggag aaattatggg agcaagtcca aatggtagac tttgtgcaaa accagtttca
    11401 gagggaattt cacctgaaaa aggtggagat actaatggac ctacagcagt tataaaatct
    11461 tgtgctaaga tggaccacat aaaaactggt ggaacattat taaatcaaag atttgcacca
    11521 tcagtagttc aaggagaaaa agggttagat aatatggcta atctagtaag agcatacttt
    11581 aatatggatg gtcatcatat acaatttaat gtatttgata aaaatgtact gttagaggct
    11641 caaaagaatc ctcaggatta taaagactta atagttagag ttgcaggata tagtgaccat
    11701 ttcaataatt taagtagaac actacaagat gagataatag gaagaactga gcaaacattc
    11761 taggtttgct tgtaaataaa acttcttact atataaaatc gtaactgata taataaagta
    11821 taaatggttt gattccattt ttaaaatttt agattaatta tgagctaact ttctaaatag
    11881 gaagttagct cgttaagttt aatgaaaaaa tcgttaaatt tatatttagt aaatcataca
    11941 catattagta agtaatacat acaaaagaat aatttattat agttataata aggttatatt
    12001 ttataaaaat aagataattt taagcagtta aaataaagtc aatttagcta ctttaaaata
    12061 aaactaatag aaaaaaggag atttgatatg aaaactttag gatttattgg ttcagggaat
    12121 atgggaagtg caatgatagg tggcatagta cagtcaggat tagtaaaggc agaagatata
    12181 actgtatcag atttaagcca agttgcctta gataaagtaa aagaagaatt tggagtaaat
    12241 actacaactg attcaaaggt agctgttaca aattctgatg tgttaatagt agcacttaaa
    12301 cctaatatat gcgaaaaggc tttaactcca cttaaagaat taatagatgc aaataaaata
    12361 atagtatcta tagcagcagg taaaactata gaaagtctgg aaagttttat aggttcagat
    12421 aaaaaaatag taagagtaat gcctaatact ccagcccttg ttggcgaagg aatgtcagct
    12481 ctttgtaaaa atagtaatgt aacagatgaa gagttaaata tgattaaggc tctgtttgaa
    12541 tcttttggtg aggcagaaat agtatcagaa tatttaatgg atacagttac aggtattagt
    12601 ggttcttctc ctgcatatgt atttatgttt atagaagcta tggcagatgc agcagtatta
    12661 gctggaatgc caagacaaca agcttataaa tttgcatctc aagcagtaat ggggtcagct
    12721 aagatggttc ttgaaactgg aaaacatcca ggtgaattaa aagatatggt ttgttctcca
    12781 gcaggaacta ctatagaagc tgttaaggta ttagaagaag aaggctttag agcagcagtt
    12841 ataaaatcta ttgttgcttg tatagataag tctaaagata tgagcaaata acagatgtaa
    12901 ttaagtttac tatatggttt cttgaattgt ttttttagtg cttatttata tctatacata
    12961 aatgtatttg gtataagtaa aataagagta atttactata aaaaggaatt taatgattga
    13021 gtgagaattt attacttaaa tattaataat taaatagata tatttttaac tatatttgag
//
LOCUS       DAGSHT010000097        12854 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGSHT010000097 DAGSHT010000000
VERSION     DAGSHT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.099"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="MJB72_002303"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="MJB72_002303"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            163..237
                     /locus_tag="MJB72_002304"
     tRNA            163..237
                     /locus_tag="MJB72_002304"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:195..197,aa:Asn,seq:gtt)
     gene            243..328
                     /locus_tag="MJB72_002305"
     tRNA            243..328
                     /locus_tag="MJB72_002305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:277..279,aa:Leu,seq:taa)
     gene            344..419
                     /locus_tag="MJB72_002306"
     tRNA            344..419
                     /locus_tag="MJB72_002306"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Met,seq:cat)
     gene            427..501
                     /locus_tag="MJB72_002307"
     tRNA            427..501
                     /locus_tag="MJB72_002307"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Glu,seq:ttc)
     gene            516..589
                     /locus_tag="MJB72_002308"
     tRNA            516..589
                     /locus_tag="MJB72_002308"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:548..550,aa:Gly,seq:tcc)
     gene            595..670
                     /locus_tag="MJB72_002309"
     tRNA            595..670
                     /locus_tag="MJB72_002309"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:628..630,aa:Val,seq:tac)
     gene            676..752
                     /locus_tag="MJB72_002310"
     tRNA            676..752
                     /locus_tag="MJB72_002310"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:710..712,aa:Asp,seq:gtc)
     gene            763..837
                     /locus_tag="MJB72_002311"
     tRNA            763..837
                     /locus_tag="MJB72_002311"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:795..797,aa:Gly,seq:gcc)
     gene            847..920
                     /locus_tag="MJB72_002312"
     tRNA            847..920
                     /locus_tag="MJB72_002312"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:881..883,aa:Arg,seq:tct)
     gene            1904..2743
                     /locus_tag="MJB72_002313"
     CDS             1904..2743
                     /locus_tag="MJB72_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBY8263403.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2740..3921
                     /locus_tag="MJB72_002314"
     CDS             2740..3921
                     /locus_tag="MJB72_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263404.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4225..5127
                     /gene="ptb"
                     /locus_tag="MJB72_002315"
     CDS             4225..5127
                     /gene="ptb"
                     /locus_tag="MJB72_002315"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBY8263405.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5149..6228
                     /gene="buk"
                     /locus_tag="MJB72_002316"
     CDS             5149..6228
                     /gene="buk"
                     /locus_tag="MJB72_002316"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY8263406.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6218..6895
                     /locus_tag="MJB72_002317"
     CDS             6218..6895
                     /locus_tag="MJB72_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8263407.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6938..7153
                     /locus_tag="MJB72_002318"
     CDS             6938..7153
                     /locus_tag="MJB72_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8263408.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7182..8261
                     /locus_tag="MJB72_002319"
     CDS             7182..8261
                     /locus_tag="MJB72_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY8263409.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8261..9013
                     /locus_tag="MJB72_002320"
     CDS             8261..9013
                     /locus_tag="MJB72_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY8263410.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9014..9571
                     /locus_tag="MJB72_002321"
     CDS             9014..9571
                     /locus_tag="MJB72_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY8263411.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9741..11087
                     /locus_tag="MJB72_002322"
     CDS             9741..11087
                     /locus_tag="MJB72_002322"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBY8263412.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11354..>12854
                     /gene="glmS"
                     /locus_tag="MJB72_002323"
     CDS             11354..>12854
                     /gene="glmS"
                     /locus_tag="MJB72_002323"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBY8263413.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFA"
BASE COUNT         4945 a         1567 c         2580 g         3762 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgttaaat gttccaaggt agctcaacgg
      181 tggagcaacc ggctgttaac cggtaggctg tgggttcgag ccccaccctt ggagccaaaa
      241 aagccgaagt ggcggaactg gcagacgcac aggacttaaa atcctgcggg acttacctct
      301 cgtaccggtt cgattccggt cttcggcact tataaaacaa tatcgcgggg tggagcagtt
      361 ggcagctcgt cgggctcata acccgaaggt cgtaggttca agtcctgcct ccgcaaccat
      421 taaaacggct cgttggtcaa gaggttaaga caccgccctt tcacggcggt aacaggggtt
      481 caattcccct acgagtcacc aattcaatta aatatgcggg tgtagctcaa tggtagagtt
      541 ctggccttcc aagccagctg tgagggttcg atccccttca cccgctccaa aaatgggacc
      601 atagctcagc tgggagagca cctgccttac aagcaggggg tcacaggttc gagccctgtt
      661 ggtcccacca ttcgcggcct ggtagttcag ttggttagaa tgccagcctg tcacgctgga
      721 ggtcgagggt tcgagcccct tccaggtcgc caaattaaat aagcgggaat agttcagtgg
      781 tagagcgcaa ccttgccaag gttgaagtcg cgagttcgaa tctcgtttcc cgctccataa
      841 taacttgggt gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg
      901 ttcgaatccc tgtgcgctca ttaagaaaag tttactataa attatagtaa acttttatat
      961 tgtttaaaaa gacacaggaa acatgtatat tacctgtgtc tttttattta taaactttaa
     1021 actatgtaaa aattacagta tattctaata gagttacagt atattcatta atattgtaaa
     1081 catacggagg gtaatgtata atagtctaca agatgtttat gaaggcatca gttaaatata
     1141 ataaatataa ctaattagta agagttatat acattatatt gtatacttta tggtatttat
     1201 atattgaaat aacttatgaa attatttata aagagattca tgagcatatt tttgtctaaa
     1261 caataagtat aagaaattat aaaaaattag agactatagt gaatttacaa tttaatgtaa
     1321 tatttataag ttatactaaa taaattatta tactgtgaaa tggatatata actttaggta
     1381 ttgtgtatta ttttcactgt aggagattat atctagttat acataataag ttttaatgat
     1441 agtttatagt aagatattaa aataaattta tctaatctag atatgaaata agttattact
     1501 ttaatataaa aatgaatcta atggaataat attgatgata agtaatattt agattttgta
     1561 tttgtttata gttaataagt ggattataga atttataata attaatatat attaaacaaa
     1621 gatatattaa gatattttag aacataaaac tgaattaagg cgcgagtact attttgtatt
     1681 cgcgccttga tggtttgagt tatttgagta aaaactaact ttaataagaa atatgtattt
     1741 attaagttga tatttttaat gtgtttagag attatttaac aatctccaaa tataaaacta
     1801 gaattattta cataaaagtt aaatagtttg tatagagtta gatttatata gtataatact
     1861 tagtgtaaat aattaagaag ttatcaaaca ggaggataaa actgtgttag atataaattc
     1921 ttttttaaat ggatttttta atataatatt aagaatgagt atatatgatt taattgacat
     1981 atctatagta gcgtatattt tttacaaaat atttatgttt ataaaagata caagagcaga
     2041 acaagtgttt aaaggtataa tatttctttt attagcaact caacttagta acacctttaa
     2101 attgcatacc gtttattgga tatctcttaa agctttagat tatggagtta tagctgctct
     2161 tataattttt caaccagaat ttagagcagg acttgaacat ataggaagag caaagtttaa
     2221 tttatttgga aagaatgtaa atacttctga agaaactctc aatagaaata tagaagaaat
     2281 tgtagaagct ttatattcac tttctagaca aaaaatagga gctcttatca taatggagag
     2341 agaaactaga ataagtgata taataaatac aggaactatt attgatgctg aaatttctag
     2401 gcaactatta ataaatatat ttataccaaa tacaccatta cacgatggag ctgttgtaat
     2461 tagagattct aaggtaaaag cagctgcatg ttttttgcca ttgacagaaa gtaaggattt
     2521 aagtaaagat ttgggtacta gacatagagc aggaataggg gtaagtgagg tgtctgactg
     2581 tataactttg attgtatcag aggaaacagg tggcgtatca attgctaaag caggtaagtt
     2641 atatagagat atttctagag aaagaatgat gaatatatta cgtagtaatt taaaaacaaa
     2701 tacagaaaca agaagttttt tcaaaggtgg tatatttaaa tgataaatag actaaaaaat
     2761 aataccaaaa taaaaataat atcattttta agtgcaattg cattatggat gtatgttatg
     2821 gctgtagttg accctgaaga aactaaaggg tttgaagata tacctgtatc tataagtaat
     2881 atgagtgatt taaaggataa aaatcttgtt atatatccag acgaaaaatt aacagcagat
     2941 gtatatataa aaggtagttt atcaaaggta caaagtgtta aaaaagaaga tatacatgta
     3001 tatggaacca ttgaagaccc aatacaagga cagaaggaag tatatttaaa agcaaatatt
     3061 cctcaaggtg ttactctaga atttaagcat gatgttttag tagtaaatct tgaaaaaaac
     3121 ataaaagaaa aaagagatat aaaggtagaa gtaagtggaa attcaaaaag aaatataaat
     3181 aaaattgaac ttagtaagga atttgtcgat gtatcaggtc caagaactct agttaaagaa
     3241 gtcaaaagtg taagagctaa tctagatgtt ggtaatgaga cagatgattt ttctaaaaaa
     3301 ttgcaattaa taccattaaa tagtgatgga actaaggttg aaggagtaac attaagttca
     3361 tcctttgtga ctgcaaagat agaactattg aaggaaaaaa ttgtcccaat aagagtaagt
     3421 ctaaatgata ataaagataa tgatgtactg aaaaactata aattaagtaa ggaacaagtt
     3481 acaataaaag gaaagaaaga agatatagac aaaataagtt atattaagac aaaacctatt
     3541 gacttagctg atttggctaa tggtgaagaa aaagaagtta gcttagaaat tccagagggt
     3601 ataactcttg aggatagctc tataacaatt aaattaaatg atacaaaaca attgagtgca
     3661 gaatttttat ataatgcggg agatattgaa ttaagaaata tcccaacaaa tatggataat
     3721 ccaaatgtaa tttctgatgt agatataaaa gttgttgtag agtcaatgga ggatttgagt
     3781 aagttaaata aaaaagatat ttcattatat attgatttat cacaaggtcc tgaggaagat
     3841 agtaaatata aaataaaata tgaaacaaca tatcaattta aaaaaataaa tatagaacct
     3901 aatattgttg aagtggaatg aacaaatatt tcgaatattt agttgttaca agatgcgaca
     3961 aaaaaaccta taaaattaag tttgttaatt ttatgttttt gttgcatctt gtttatttta
     4021 gttatattat aaaataagga caatgagata tttgttaaat ctaaaaagcg ctagatatat
     4081 gaaaaaagac tgataaatag atttaaataa atattttgaa ttaaatattt caattatctc
     4141 taggaaaacg ttgctgaacg agttataatt taattgagat ataaaaagtt ctatttcaaa
     4201 aaattcaaga tgaggtggaa atgcatgaga agttttgaag aagtaattaa gtttgcaaaa
     4261 gaaagaggac ctaaaactat atcagtagca tgttgccaag ataaagaagt tttaatggca
     4321 gttgaaatgg ctagaaaaga aaaaatagca aatgccattt tagtaggaga tatagaaaag
     4381 actaaagaaa ttgcaaaaag catagacatg gatatcgaaa attatgaact gatagatata
     4441 aaagatttag cagaagcatc tctaaaatct gttgaattag tttcacaagg aaaagccgac
     4501 atggtaatga aaggcttagt agacacatca ataatactaa aagcagtttt aaataaagaa
     4561 gtaggtctta gaactggaaa tgtattaagt cacgtagcag tatttgatgt agagggatat
     4621 gatagattat ttttcgtaac tgacgcagct atgaacttag ctcctgatac aaatactaaa
     4681 aagcaaatca tagaaaatgc ttgcacagta gcacattcat tagatataag tgaaccaaaa
     4741 gttgctgcaa tatgcgcaaa agaaaaagta aatccaaaaa tgaaagatac agttgaagct
     4801 aaagaactag aagaaatgta tgaaagagga gaaatcaaag gttgtatggt tggtggacct
     4861 tttgcaattg ataatgcagt atctttagaa gcagctaaac ataaaggtat aaatcatcct
     4921 gtagcaggaa gagctgatat attattagcc ccagatattg aaggtggtaa catattatat
     4981 aaagctttgg tattcttctc aaaatcaaaa aatgcaggag ttatagttgg ggctaaagca
     5041 ccaataatat taacttctag agcagacagt gaagaaacta aactaaactc aatagcttta
     5101 ggtgttttaa tggcagcaaa ggcataattt taagggggaa ataggcaaat gagcaaaata
     5161 tttaaaatct taacaataaa tcctggttcg acatcaacta aaatagctgt atttgataat
     5221 gaggatttag tatttgaaaa aactttaaga cattcttcag aagaaatagg aaaatatgag
     5281 aaggtgtctg accaatttga atttcgtaaa caagtaatag aagaagctct aaaagaaggt
     5341 ggagtaaaaa catctgaatt agatgctgta gtaggtagag gaggacttct taaacctata
     5401 aaaggtggta cttattcagt aagtgctgct atgattgaag atttaaaagt gggagtttta
     5461 ggagaacacg cttcaaacct aggtggaata atagcaaaac aaataggtga agaagtaaat
     5521 gttccttcat acatagtaga ccctgttgtt gtagatgaat tagaagatgt tgctagaatt
     5581 tctggtatgc ctgaaataag tagagcaagt gtagtacatg ctttaaatca aaaggcaata
     5641 gcaagaagat atgctagaga aataaacaag aaatatgaag atataaatct tatagttgca
     5701 catatgggtg gaggagtttc tgttggagct cataaaaatg gtaaaatagt agatgttgca
     5761 aacgcattag atggagaagg acctttctct ccagaaagaa gtggtggact accagtaggt
     5821 gcattagtaa aaatgtgctt tagtggaaaa tatactcaag atgaaattaa aaagaaaata
     5881 aaaggtaatg gcggattagt tgcatactta aacactaatg atgctagaga agttgaagaa
     5941 agaattgaag ctggtgatga aaaagctaaa ttagcatatg aagctatggc atatcaaatc
     6001 tctaaagaaa taggagctag tgctgcagtt cttaagggag atgtaaaagc aatattatta
     6061 actggtggaa tcgcatattc aaaaatgttt acagaaatga ttgcagatag agttaaattt
     6121 atagcagatg taaaagttta tccaggtgaa gatgaaatga ttgcattagc tcaaggtgga
     6181 cttagagttt taactggtga agaagaggct caagtttatg ataactaatg ataaaagaat
     6241 aagaatcata actggtcact atggaagtgg taaaagtgaa tttgcgatga actatgttgt
     6301 taagttaaga gatatggttt ctggcaaagt tgctatagcc gatttagatg tggtaaatgt
     6361 atactttaga actagagaaa agaaagagtt aatgaaatcc ttaggtatac aaccaataga
     6421 tagctcaatt aatgctccaa cattagattt accagcagta tcagcagagg taatgtctcc
     6481 tatggtagac cattcataca attctgttat agatttaggt ggagataatg taggtgctag
     6541 ggttatagga agatttagtc atttgttaaa agaaggcgat tatgatatgc tttttgtaat
     6601 aaatgcaaat agggagaaaa ctcaaacttc tgaagaagtt atccaatata taaaagaaat
     6661 agaaaaatct tctaaattaa aggtaacagg tctaattaat aatactcatc ttattaggtt
     6721 tactactatt gatgatgttt taagaggtca gaaagttgca aaagaagttt ctgaaaaatg
     6781 taatataccg ataagatatg ttgcatgttt agaaaatctt gttgaacagt taccaaaaga
     6841 tttagaagga gaaatttttc ctataaaatt atatatgaga gaagactgga tgtagtttga
     6901 gctcaaatat atagaaagtt taaggaggct taggtaaatg gctaaaggaa aagtatcttt
     6961 taatcaagaa cgttgtaaag gttgtggatt gtgtgttgaa gcatgtccag taaaaatcat
     7021 acaattagac tcaaatgtaa taaacaaaaa agggtataac ccagcaactg tttttgaaat
     7081 ggaaaaatgc ataggatgtg caagttgtgc aactatgtgt ccagatgtag ttataacagt
     7141 agaaagagat taatttaatt aatatttaag ggggaatttt aatggctaag atacttatga
     7201 aaggtaacga agcatttgga aaagcagcta tagaagctgg ttgcaaatat ttcttcggtt
     7261 acccaataac tccacaaagt gaattaccag aatatttatc aagagagtta cctaaaatag
     7321 gtggagcgtt cgttcaagct gaatcagaag tttctgcgat aaacatggtt tatggtggag
     7381 caggtgcagg agcaagagtt atgacttctt catcttcacc aggagttgct ttaaagcaag
     7441 aaggtataac atatgcagta ggagcagaag ttccttgtgt tgtacttaat gtaatgagag
     7501 gtggtccagg acttggaagt atacaacctt cacaagctga ctactttatg tctacaagag
     7561 gtggaggaaa tggagactac agaactccag tattcgcacc agctacagtt caagaagctg
     7621 ttgatatgat aatggaagct tttgacgttg cagattatta cagatcacca gttatggtag
     7681 ttgcagatgg tatgataggt caaatgatgg agccagttga atttagagct cctgagaaga
     7741 aaagagaatt gcctcctaaa gattgggcaa ctgttggaac taaaggaaaa agaaaaccta
     7801 atgtaataaa ctctctatac ttagagccag aagtactaga agaccattgt tggcatttac
     7861 aagaaaaatt tgatgcaatg gaaaagaatg aagtacaata cgaaatgtac aaaacagaag
     7921 atgctgaatt tgtatttgca gcttatggaa ctacttcaag agtagtaaaa agcgcaattg
     7981 atatattaag agaagaagga ataaaagcag gtcttataag acctaaagta ttatggccat
     8041 tcccattcga agcattcaac caaattccta atgctagaaa catattgact gttgaaatga
     8101 gtatgggaca aatggtggaa gatgttaaaa tggcagtaga aggtaaatta ccagtttact
     8161 tccatggaag accaggagga atgactccaa caccagcaga aatagttgaa aaagctaaaa
     8221 aaattattgc cggagaattg gttgcaggag gtgctagata atggctgttg tatttaagaa
     8281 aactgaagga ttacaagata ctcaaactca ctactgtcca ggatgtacac atggtataat
     8341 ccatagatta gttggtgaag tattagaaga actaggagta ttaggtgatg ctgtaggtgt
     8401 tgttccagtt ggatgttcag ttttaggata taaatatttt aactgtgaca ctcaagaagc
     8461 tgctcatggt agagcgccag ctgctgctac aggaataaaa agagttcatc ctgaaaatac
     8521 agtattcact tatcaaggtg atggagactt agcttcaatt ggtactgctg aaatagttca
     8581 tgcagctgct agaggagaaa aaataactac tatatttgta aacaatacaa cttatggtat
     8641 gactggtgga caaatggctc caactacatt agttggacaa agagctacta ctgctcaatc
     8701 aggaagaaat gctgaaactc aaggttatcc aataagagta agtgaaatgt tagctacatt
     8761 aacaggagct gtatttgtag aaagagtagc agttgatact ccagctcatg ttagacaagc
     8821 taaaaaagct ataaagaaag cattccaagt tcaacaagca ggattaggat ttggaatagt
     8881 agaagtatta tctacttgtc ctacaaactg gggactagct ccaaacgatg ctttacaatg
     8941 gttaagagac aacatgatac catactatcc attaggaaat ttcaaaaatg ttgaagttga
     9001 ggaggtgaag taaatgtcta cagctagagt aatatgtgca ggcttcggag gccaaggtgt
     9061 tatgtctatg ggacaattac ttacttatgc aggaatgcta gaaggaaaag aagtttcttg
     9121 gttaccatct tatggaccag aaatgcgtgg aggtacagca aactgtgcag taactgtatc
     9181 taatgagcca gttggttctc cacttataac agatgatgca actgcagcta tattattaaa
     9241 tataccagca tttgaaaaat tcaaagatga tgttattcct ggtggaaaaa taatagtaaa
     9301 tagttcttta ataaaagaaa aagttgatag aacagatgta gatgtttatt atataccagc
     9361 aaatgaatta gcagctgaat taggaaatga taaagttgct aacatgataa tgttaggcgc
     9421 ttacttaaaa gtatctgata ctgtagatat agaatctgta ctagaagcat tcaaaaaagt
     9481 tttcggacct agaaaagaaa aattcgttcc attaaacaga gaagctttac aaaaaggtat
     9541 ggatgctaca tgttctaaag caactaacta ataaagaaaa agatgtcaaa aaaaagacaa
     9601 tagaattatt tctattgtct tttttgctga taagtattaa aatattttta tgactgaaat
     9661 taagcctata atgatatttt atgaactata aaaatataat acaaattaca agcttttcaa
     9721 ttatgaaagg agaatcttta gtgagaaagt attttggaac agatggagtt agaggagtag
     9781 caaatacaga actaacatgt gatttagcat ataaacttgg tagagctggt ggatttgtct
     9841 tagctcaagg agaccacaga gtaaaagtag tagtaggtaa ggatactaga atatcaggtg
     9901 atatgttgga agcatcttta atagcaggac ttatgtctgt tggatgtgat gttattactg
     9961 ttggaataat accaacacct gcagtggcat atttaactag aaaatatggt gctgattgtg
    10021 gtgtggttat atctgcatct cacaatcctg ttgaatataa tggaatcaaa ttctttaata
    10081 agaatggata taaattagat gatgaaattg aattgaaaat agaagaatat atagacgata
    10141 tagacaaaat tgattgtctt ccaattggtg aaaatgtagg aagaaaatta catgaacatt
    10201 gtgcacaaag agactatgta gactacctaa aatcaataat aagcactgac tttaagggat
    10261 taaaagtagt tttggactgt gctaatggtg cgtcatataa agtggcacca atagtgtttg
    10321 atgaactagg agcaagtgta atttcaataa atagttcacc agatggaaat aatataaact
    10381 ataaatgtgg ctctacacat ccagaacaac ttcaaagggc tgttttggaa cataatgctg
    10441 acttaggtct tgcatatgat ggagatgctg atagattaat tgcagtaaat gaaaagggtc
    10501 aaattgttga tggagaccat ataatgatat tgagtgcctt aaatttaaag aaaaataata
    10561 aattggcaca agatactttg gtagttacag ttatgagtaa tatagggttg acaatagctg
    10621 ctaaggaaaa tggaataaat ttatctacta cagcagttgg agacagatat gttttagaag
    10681 atatggttaa aaatggatat aatcttggtg gagagcaatc tggacatatg atatttttag
    10741 attacaacac tactggagat ggagtgctta gttctctaat acttgcaaat ataatcttgc
    10801 aagaaaagaa acctttatca gaaattgcat caataatgag ccaataccca caagtattag
    10861 taaatgcaac aataaaaaat gaaaataaga acaaatatat ggaatatcca gaaataaaaa
    10921 ctgagataga aagaatagaa tctattttag acggaaatgg tagagttttg ataagaccat
    10981 caggaactga gccattagtt agagttatgt tagagggtaa agaggaaggt caaataaaag
    11041 aattagcaac aaacttagct aatcttatac aagaaaaatt atcgtaattt aaagggtatt
    11101 ctcagatata aatactgagg acacctttct aagcgccaga acttagttat agacataatt
    11161 attgagtaat ataggaacta agttgacgag gtcagagtta atcgaattgt cggcggatgc
    11221 tctgcggtgt gtttaccatc gaaagacttc tttaaataat aggagcaatc ctatggacaa
    11281 agggaagcca aagtaaactt atatgaccta actcttaaaa tatataaata atatttttag
    11341 gaggacacaa ataatgtgtg gaatagttgg atatttagga agtagaaagg cagcagaagt
    11401 tatagtagaa gggctttcta agctagagta cagaggttat gactcagcag gagtagcagt
    11461 taatagttct aatgaaaaag aacttaatat tagaaagttt aaaggtagac tttctgtact
    11521 tgcagaagac ttagaaaaga atcctattga tggaaattta ggtatagggc atacgagatg
    11581 ggcaactcat ggagaaccat ctgatgtaaa ctcacatcct cattttaatc aagccaaaac
    11641 aatagcagtt gttcataatg ggataataga aaactatatg gaaataaaag aagaattaat
    11701 ttctgaaggt gttaagtttg aatctcaaac agatacagag gtaatagcac atttagtaga
    11761 taaatactat gagggtaatt tattagatgc tgtatacaaa actatatcaa aattaagagg
    11821 agcttatgca ttaggtgtta tatgtaaaga acatggaaat gaattagttg cagtaagaaa
    11881 agatagtcct cttgtagttg gtgttggtga aggtgaaaac tttatagcat cagatatacc
    11941 tgcattatta aaatatacta gagatgttta tttcttagaa aatggagaag tagttcatct
    12001 aaaagacgaa aatgtaacag tttatgactc taatagaaat cttgttgaaa aagaagtatt
    12061 tcatgtaact tgggatgtag aagctgcgtc taaaggtgga tatgactact ttatgtcaaa
    12121 agaaattcat gagcaaccaa caggagtaag agaaacttta gaaagaagac ttgatgataa
    12181 tggaaatata attctagaca gtataaatat ttctaaagaa gacttagaaa aaataaataa
    12241 agtttatata gtggcttgtg gaacagcata taatgctgga ttacttggta agtatgccat
    12301 agaaaaattt gtaaatatac cagtaataac tgatatagca tcagaattta gatatagcga
    12361 cccatttgta gatgaaaatt cattagtaat attagttagt caatctggtg agacagcaga
    12421 tactctagca gtattaagag attcaaaggc taaaggagca agaatacttt caataactaa
    12481 tgttgtaggt tcatctatag caagagaatc agatgatgta ttctatactt gggcaggacc
    12541 tgaagtagca gttgcatcaa ctaaggcata tactactcaa ataacttctt tatacatgat
    12601 agctttagac tttgctatca aaaaaggaac aataactaga gaattttatg atagtatgat
    12661 ttctaagatg aaagaaatac catcaaaaat acaagaaata ctagacaatg aagaatatat
    12721 aaaagaagtt gctaaaacag ttgttagttc tgaacatgca ttctatttag gaagaggtat
    12781 agactatagc ttagcaatgg aaggttcatt gaaattaaaa gagatttcat atatacatgc
    12841 agaagctttt gcag
//
LOCUS       DAGSHT010000098        12802 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGSHT010000098 DAGSHT010000000
VERSION     DAGSHT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.100"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(112..273)
                     /locus_tag="MJB72_002324"
     CDS             complement(112..273)
                     /locus_tag="MJB72_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263414.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(601..1041)
                     /locus_tag="MJB72_002325"
     CDS             complement(601..1041)
                     /locus_tag="MJB72_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY8263415.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASDRFVDENGKPVEWELKVLSSE
                     EDESLRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1113..1583)
                     /locus_tag="MJB72_002326"
     CDS             complement(1113..1583)
                     /locus_tag="MJB72_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBY8263416.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1600..2910)
                     /locus_tag="MJB72_002327"
     CDS             complement(1600..2910)
                     /locus_tag="MJB72_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBY8263417.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENV
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(2911..3087)
                     /locus_tag="MJB72_002328"
     CDS             complement(2911..3087)
                     /locus_tag="MJB72_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263418.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3080..3517)
                     /locus_tag="MJB72_002329"
     CDS             complement(3080..3517)
                     /locus_tag="MJB72_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263419.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(3866..4672)
                     /locus_tag="MJB72_002330"
     CDS             complement(3866..4672)
                     /locus_tag="MJB72_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263420.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNNLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(4782..5072)
                     /locus_tag="MJB72_002331"
     CDS             complement(4782..5072)
                     /locus_tag="MJB72_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBY8263421.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5072..5419)
                     /locus_tag="MJB72_002332"
     CDS             complement(5072..5419)
                     /locus_tag="MJB72_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263422.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(5413..5814)
                     /locus_tag="MJB72_002333"
     CDS             complement(5413..5814)
                     /locus_tag="MJB72_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263423.1"
                     /translation="MENNLIDEIEKRLESFGYILKDGDKWLIDFIREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNNILTFRCLRW"
     gene            complement(5824..6057)
                     /locus_tag="MJB72_002334"
     CDS             complement(5824..6057)
                     /locus_tag="MJB72_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263424.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6057..6956)
                     /locus_tag="MJB72_002335"
     CDS             complement(6057..6956)
                     /locus_tag="MJB72_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBY8263425.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGVGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGV"
     gene            complement(6968..7531)
                     /locus_tag="MJB72_002336"
     CDS             complement(6968..7531)
                     /locus_tag="MJB72_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBY8263426.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(7597..7797)
                     /locus_tag="MJB72_002337"
     CDS             complement(7597..7797)
                     /locus_tag="MJB72_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263427.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            complement(7812..9209)
                     /locus_tag="MJB72_002338"
     CDS             complement(7812..9209)
                     /locus_tag="MJB72_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBY8263428.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIRHGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFAKSFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            complement(9199..10638)
                     /locus_tag="MJB72_002339"
     CDS             complement(9199..10638)
                     /locus_tag="MJB72_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY8263429.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRRGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFISFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(10644..11897)
                     /locus_tag="MJB72_002340"
     CDS             complement(10644..11897)
                     /locus_tag="MJB72_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBY8263430.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(11875..12558)
                     /locus_tag="MJB72_002341"
     CDS             complement(11875..12558)
                     /locus_tag="MJB72_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8263431.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPKETRELIQEISTKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMVKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
     gene            complement(12632..>12802)
                     /locus_tag="MJB72_002342"
     CDS             complement(12632..>12802)
                     /locus_tag="MJB72_002342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263432.1"
                     /translation="FYIVVPSIELLSLLLTLIVFFKATRDVWFIIKIVRNKIKKSVPS
                     DDDLNETIRLIK"
BASE COUNT         3680 a         2485 c         1314 g         5323 t
ORIGIN      
        1 tttttatttt ttattttata taaacaatat tttttctaat ttgtggtata ataaaagcaa
       61 gaagaactac aatctatttt gcggtagagt gaagttcata attaaatgaa tctatttgaa
      121 cttatggaac ttgattttaa aatcaaattc ccagccactt ttactcttgc cacgagttga
      181 gtggcttttt acttttagaa atactttaca aattaagcaa aatattaaac tagcaataac
      241 gccagctatt acattaagta aaaagttatt catacttccc acctcctttc attaggaagt
      301 aggttttatc ccagtatgaa ctccactcta taaattgtag attacatctt cttgctaaaa
      361 atattataac atattttacc tattctatat ttattttttt attttgctat cttcttcgtc
      421 ccctctttct ctctctttca gcttctttca ttgcttcctc ttcatcttct atctttataa
      481 gtattgaggc ggctgctaac gctctctcat taacttctaa attcatatat tcacttggct
      541 tccactttaa tttttgaata caataatgag tgatgctagc atcaaaatcg ccacctctga
      601 ttagtttttt gcttcttcta ctttatcctc aaaagatgta tcaaatccat tgacttcatt
      661 cacttttact gtataattga catactcacc tgctgtaagc attgtcttta ataactgagc
      721 ttctcccatc actccataac tattttggag ttcggcatcc tttaaatctg gaaatactgt
      781 agatgctaca cataattcag ctacataact gttataatca atttcacttg tatattgacc
      841 tgtatgttta ccattgttgc ctataacttt cactctttta gtgcattttc ttcttaatga
      901 ttcatcttct tcagatgata aaactttaag ttcccattct actggttttc cattctcatc
      961 tacgaatcta tcacttgcca catattttac attatcaacc tttattgcat tttgacttaa
     1021 aaaagcactt aaattactca tattattcta atctcctttt attttaattt ttcatataaa
     1081 aaatacacat atataattta taaatgtgta ttttactcca ttcctgccaa taaattaaat
     1141 ttttctacta attcccaatc ctcaaaagtg aaatccatat cttcatctaa atactcacca
     1201 tcagcatcaa atttagtaat tattccactg tccatattac aatctttaag tactactgtc
     1261 tgtcttccta cagcagatgt agggtcttca tttgtaactt gtatgtcaaa ataaatatcc
     1321 tcaccagttt ctttatatct gtaaagtaat tctctaaaaa tagaagtatt ataatgaaat
     1381 gttgcacttc cagtatttgt actcccagtt gttttatttc cctttgttgt tcttcctaga
     1441 attggaactt cacttttatt tttttccatt ttagcctcta aatctatagc ttgcatgaag
     1501 ttatatcttt tgccttctat agttataaaa cattcagctt tctttgcact aactgtatct
     1561 ttagcattta ttgtttgagc catattatca ctctcctctc taactaactg aaacagtcat
     1621 ataaagctta ctcatagcat ttattacctt aacagcatca gatactatga cagttttctt
     1681 atcatttcca agctctacac taacatcatc agttttaaaa tcttctattg cccttacatt
     1741 ctctaattct ttatggtgtt taacaacatc attccagaaa cttattcttc ctgccttatc
     1801 attcggaact ttacctaaat acttttcatt aaataaagtt gcaatatcat tagcaatttg
     1861 gtcaagtact ctaacacttt ggttacttga aaaatcgtca tttttatcgt ctgtaaatga
     1921 tacaaaagta tttatatcct ctaacacatg aacttcatct cccaccttgt ggaatataaa
     1981 tttaccagtt tttaaagctt cttcaagttg tatttgtgta taattaacat caacatcaaa
     2041 ctcaccatca tactttttat tagtattaga tttatttata tcgcatcctg ctatagctcc
     2101 agccgcccaa taaattaaac tagattctac taatccaata tctttaatct tattttctac
     2161 agacactaca ccttcataat ctgcatcact tttcttatat agtactgttt gaaacttagc
     2221 tcctacctta tctctcattc tctttgtaaa ttctacaaat aaacttttaa tttctgttgt
     2281 tgtagccaaa catcctaaag cattaaagct atagctttct attttatcca agaaagcttg
     2341 gtactctgct cctgtcacag cttcgccatt agttccacca gtaaatacca gccctgcact
     2401 tgcttctagt gttgcatcct tcttccaagt gatatagtca ttatcttgta agtctgtaat
     2461 cactttagct atttgagtat ctaccttctt attatctaaa agtgttacaa catcaaactt
     2521 agcattatca tctatatttg ttgtaactgt tacttttaag tcatttcctc tgataccact
     2581 atattttgct gtggctatag tacaactggc tttaacgcct ttatttaatt tataaaaata
     2641 tcccaacctt atatttttga ataaatctct caaacctttc agcttctcat gagtataatc
     2701 atatccaaaa tacttcactg aatacttctc aaaatcatca ctggttactt gaaatacgtc
     2761 ttcatctatg ccccaatcta actctaaagg tattgcaaca atacctctat ccgataatga
     2821 actggttgcc ctcttagctg agataaaatt tatatagcta cctggtaata ttttattctg
     2881 tgttacaaat gttcctccac ctaaagccat ctaactcact cctttcataa atttatttat
     2941 tctatcctct acttctgaga aggaatataa atcattttct tttaaaattg catttaataa
     3001 atcttttcta tttacatact tcttagagtt aactatctgc tccttagtaa acttgtagtt
     3061 atcttctttg cttaatgttt tactcaaaat tatcacctct cttcaaacca ccgaataact
     3121 ctactgtatc catcttatct gtgtcattac tttttatagt aaagtagtta taatcaacaa
     3181 agaaatgaag aacattatct ataatttcaa agttcatatt tgtacctctg actaagtctc
     3241 catcaatttc tatatactct aattcctcca gtaacatctc agctatctca tttatttcaa
     3301 atgatttatc attactttta ggaaaataat gtacatcaaa agagttcttt tttaatgtcc
     3361 tgccacttgg ataggatact ttgcttggat ttaaaggaac aataaaaaaa caaggttcat
     3421 ttataccttg ctccacatct tcactataaa ttgtataact ctctccaaat gttttatcta
     3481 atttaataga tatcccatca attatattat taagcatcaa atactccttt aagcaatatt
     3541 aatagttttt tctctataat cttatcaact tggctttgta gttccatctc tgaaattgtt
     3601 aagaaatgtt gtcctttaac ccatccctta ccacttttag ttctatgccc aaactcaaca
     3661 tatgcatttt gttatcctaa aggcttttta tcctctagct cttatagttt cctataagtt
     3721 cggcgtacat catcaacaaa ataaacttta tttagttgcc cagcactctt ggagagatta
     3781 tatttattca ctctctacgc tctacggaaa cctatagcct attcgcaatc tataggtttt
     3841 cctcggtatt ggcatatata attaattaaa tatttttcca tgttcttcta tggactatat
     3901 ttgatataga tgaatatgtt acaggatata tttcacttag ttgttttatt gtatatccat
     3961 cagaatactt ttttctaatt tcttttacat cctcaataga taacttagct ctgctttctt
     4021 caataatctt aactttttct ataagattat ttcctactaa tcccttagaa tatctacttc
     4081 ttaagcaaga atatgaaata cctgtttttt cagaaagctc tatcaatgta atttcttttt
     4141 cttcatgctt tactaatata tttgttgacc tatttctaca ttgtgtcttt atatccaccc
     4201 atctacaatt gcttgattca taatttcctt cattatttat tctatcaatt gtaagtttct
     4261 ctgaatatcc attactcaat gcccaatttg caaaattatc atagcaaaac cattcatcac
     4321 acacaattat attcctttca ccatatctat tgtatctttt attattttta tctgtacatc
     4381 tagatattat acccttccat atactgtaaa gtcttttatt ttgaacctta tatttctttt
     4441 taaattggta tttgtctgtt aaatttaatt tatcttgttc tttttttaaa catccacaag
     4501 attgtactaa gccactagtt aaagagtcag ttcttatttc tttgaaattt ccacaatcac
     4561 acttacataa ccaatatttt ctatttcttt taccactttt tatctcttta gaaaatttaa
     4621 tgactcttag ccttccaaat ttttttcctg taatatctaa aaattttgcc ataaaatcac
     4681 ctcttttata cattataaat caagagttat tctatgtcca tattctacct actttataat
     4741 gtaacactat tacacttagc gtttaccgat tttgctgggt tttatatgcc ccattgtgtt
     4801 aaggcatatt cagtcggatt aacaacctct ataatataat tatttccttg tttatacaca
     4861 ggaagcgacc tagcataagc cactccattc catccttgtc gtaagaatcc tgtatcaact
     4921 ggtgttcttc taattacttt cccaagtaat cgtgctgcta attctcttgc tgcatccttg
     4981 caaaacttat ctaaatcaat ctttgtaagc tcctccatct ttttacaaac tcttttaaat
     5041 tctctaaaat caacactgcc ccatctagcc attatgcttt atccttaaat aactcaagta
     5101 ttatttcttg atgatttgga tatatagctg attctccact tcttacatac tctttatcat
     5161 ttataataag ttttgaacct gctttaattt ctatatctgg agatataaag agtttaatag
     5221 tttgctctag cttagctact ttcccttctg tagcagaaac tatattttta tatgaaagtt
     5281 tacatggttg attttctaat acaatcactt ctttattgtt agttcgtttt gttacagggt
     5341 ctttaattgg ctgatactca actatagtac atttatctct atataacatt tctattgctt
     5401 ttctagtttt acttaccatc ttaagcacct aaaggttaat atattattct taccataagt
     5461 agtaagataa gctattaagc tatcaaagcg ttgttctggt gtttgagaac cacttcctat
     5521 agcaaaatct acctttgtat caccttctga tatagacttt tctacagctt caaagttaat
     5581 gctttctata tctaattgac ccatattttt cttggtaaat aagaactctc caactatcat
     5641 atcaacttca atttctttca attcaattgg catagttttt atattacaat ctagtttaat
     5701 aatattttct attttttctc ttataaaatc tattaaccac ttatctccat cttttaatat
     5761 atatccaaaa ctttcaagtc ttttttctat ttcatcaatc agattatttt ccataatttt
     5821 cacctacttt ttagtaagct tatttttctc tttaagctgc ttattttctt cttctaaaga
     5881 ctcaactttt gaccttaaaa tattattttc agctattaaa tcttttacat ttaatgactt
     5941 gccatacttt actgccttac cagtttcatc tatcaaatca tatcccatct ctaagaaatc
     6001 atctatttta cactcttcta tagttaatat tctatttaat ttccttactt gtgccattat
     6061 actccagctc cttcaacaac aaattgtatt gcatcagctt ttttatttaa tataaataca
     6121 tcctcaaaac tttcttcaaa gtagaagtat tttccctctg taactgctgt tggttcgtct
     6181 aacttagaga actgataaga aacaggtgta attattgcac ttgggtgaac taaggacata
     6241 aagatttgtt tagctcctac tcctactttc catccagttg taaaatcata tgcagttttc
     6301 attagattag atggtacttt aattatttta actgtgtcaa tatctgtggt ttgtctattt
     6361 aaagaagttc caccatcttt tatgtttact gttctttgta tctcttttgc atttttgata
     6421 agtgtattta ctactggagt aacatacaat attcttccat tttcaggtac tctagcttct
     6481 gtcatttttt ccattaactt atcaaatact tctaatacgt ttgctgttgt aagaacagtt
     6541 gtatctgctg tgttacctaa tgcggtccaa tcagcatata ttttagatat acaataagca
     6601 tccatctctg gaaacttttg ttcctcatta tatacttttg ttatattgcc tattgaagcc
     6661 acataattag tttggtttat atctgctgga tgaaccaatg ttgaccattt cctttgatta
     6721 gttaatacct taggttccca agcattatca tagtttcttt gagctactgc tattgtatct
     6781 ctgtttgaat ctactcttcc agttgtagat atagttggta tttctattgt tttagaacca
     6841 gtccatctat atcttccatt atttggtgtt gcatacaaat ccccgaagtt taaagtataa
     6901 ggatatgctt gtgctaaaac atttgaatat tcttttgcat aatttagtgc tgccatttta
     6961 tttcctccta tttattatta ttttcatgag gtcttacccc agtaaaatta aaaccaaaat
     7021 catttatctt aggctcttgc cctggtgtta tagtatctat tttaggttct tcaccttcta
     7081 gtgttgcatt aaacaaataa tctttatcct gtttcaaagg atttatttgc tcttcaaaag
     7141 ctttttgtct gtctttacta tttcttagtg cttctatatc taaatgagct tttaatgcta
     7201 tttcatctct acatttaaca gatttaaaag catcacctaa ccagtaatta aaatcttttt
     7261 cttcaatttc ttttttgtag gtttcttcca aagttttctt atcagtttca taagttgttt
     7321 ttagattctc tacatcttct tttgtcatac ctccttcaaa ctttttaata gtttcattag
     7381 ctgtattaag ctgtgtttca agatttgtat aatcttcttg agtaattgta gtctctttta
     7441 ttttcttttc tatagatttt tgaagagaag ctacatcaat tttgttatcc tctactttta
     7501 ttccttctag caattctttt aaccaatcca ttttaaattt ctcctttcat tttttacaaa
     7561 ataaaagcat ctacttattt ttaagtacat gcttagtcat tccttattta tattttcgat
     7621 agattctatt tcattttcat aaacttcaat tccataacca tccctagcta ttgatatact
     7681 tgctatttct ggttcattat ctaaagcttg tgtatatcca tcacactttc ctcttattat
     7741 ttgcttatct acacaagtta tttgaacatt tttccctaca tattcccata atttcatttt
     7801 atttttcctc cttataaagc tggtactata tgtgttccag ttttggaata atgtatctta
     7861 aacttatttg taagagtttt ttcacccgta atattattaa cattgacccc tatattctta
     7921 tcaacttcta taagttcttt tttatcccat tctccacttc gattaaattt tatgattcca
     7981 ttgccagcat gcttattcac aagttcttga gcttcttctt ttgttatagt taaatagctt
     8041 cttccttcta tataattatt atgctctttt aaatgttttc cttgtttccc atcatgaata
     8101 tttaaattat atttaccatt tttaatatct tcttttatgc tatctattat agcactattt
     8161 tttatttcta agatactatt atgtttaaca tacttctcat accactcatt atacttcata
     8221 ctagatggta cataatatgt ttttccatct tctccttttg ctgctctgta accttcttca
     8281 tcctcaaacc aaggagctgt tgttgtccta caacgacaat gaaatggtgg agctgttatt
     8341 ccaacttgat aatctttcat atcaaatact tttccatcta actctctgca tatatttgaa
     8401 gttcttaaat ctaatgtagc aataatctca tatttctcta catctaaatc attaaaacaa
     8461 tcttttctac ttgctgatgc aaagaaagct gattcagtca ttattaaatt cttagcttgt
     8521 gatttagata cattaaagct cttagcaaag tcattgacta gattttttgg attctcacct
     8581 ctaataattg attgtgtcaa tttagtgtgt aactcattaa ttaaagcagg tctatgtttg
     8641 ccccaaatcc tttcactaaa atttaatcca tcactagtcc atggtttaga gataacttta
     8701 tttattctat tagtatcaag actcattaaa ctccaaccaa cgtttactcc ttgttgaaca
     8761 ttaaaagctg tatgatagta tccacttgta taaatatctc tcattagttt atcaatacca
     8821 tcaagttcat ttccatataa aacttctact tgttgctgta tttgtaactt taaagcttca
     8881 agtcttgtta tatgaactct tgcactagca ttttctaact ctttcatcca cttttgattt
     8941 atagcatttt ctttaccatg tctaatatat tcttcgacac tccatttaaa ctcttctagt
     9001 tctcttgtat ttagtagttt cttagcttct aataaagata ttccttcatt tttggcaaat
     9061 ctgttgtacc atgctaatat atctttttct atactattca tagctagttt atattgcttt
     9121 tctaattcaa gataatattt tacacttttg ttattttgag cttcttctaa ttgttcaaat
     9181 ctcttcctcc aataatcttt atgtttcatc tataacacca tcttgattat taggaattaa
     9241 atcatcatac tctttttgag tatcttcctg ttttttaagt ctctcaagtt cgtcatttac
     9301 atcctcgacc caaggatggt tagaaacaat agtttcatct gatacaattc cagttgattt
     9361 agctgccata tctatctttt cagcttcatt tattatcata gagtgattaa aagtaatttg
     9421 aactgtttta taatcatagc tcttactacc acttatcttt aaatactcac acacaaacca
     9481 taaaagctct ctaattgctt ttttaaactt cttttcagtc ttagaacatt taaggtccag
     9541 taatgaatat aaaaatttaa gtgctacacc cgatttgtca cctgtgtttt gagattctgg
     9601 attaactcct tgaccaaaga taattatatt cttttctaat ctatcaagaa gctccttttt
     9661 agcttcaact ggtatattta tctctagttt atcaactcca cctccaccat ctactttaat
     9721 tgatttatag tatcttatat tatctataaa ctcttgtaga cttgttcctg gatattcttt
     9781 taatacataa ataacctctt gtatttcatc taagttatct gctagtgtag aaatattatt
     9841 gtcatatata tctattaatg atttatagaa agttaaatct gagacacact tttcattatt
     9901 tttaaaagat ataaatggaa ctttacccca tccctgttct ttgttattta ttctaaaatg
     9961 accttcttgt atatcagtca tttttccata ttcatcatat aaaaattctt gaataaaact
    10021 attacctctt tcaataaagt aagttacgtc attttctgtg tagtactcaa ctctttttat
    10081 tttatttcca tctatatctt caatataata aaacctaata aatgcaacta attccctctg
    10141 tcttttacta tcccaaatag gaattgcttc ttcagctgga attattacat atttaaactc
    10201 acctcttcta ttaatatatg gatgtaacca ttcaacccct ttattactag cattgagata
    10261 tagttctgtt attgtatcgt caaactcttc tcctagtaag tcatttaaaa gcttagtgag
    10321 attatcatca tctgcattaa atactatggg atttccgaca ctatagccta ccttttggtc
    10381 aactaaaagc ttatggtagt tgttaattgc tttattatta actttagtaa aatcatcaac
    10441 cttagctcca tctaagagat aatatcttct cttattgttt acatcagtat taccataata
    10501 gtattcttct ccttgtttat atttttctgg cctatgtttt aagatgtagt gttctatgac
    10561 ttttactagg ttaaaggtgc tctctttttt taactgaact tttattaaat ctgtttcact
    10621 tatataaata tttaacacct cctttacttt aagaagctta ttccattatt tttaagctta
    10681 ttatctatag aatatctaag agcagccatt gcatcatcca taaactcaac tggttcatca
    10741 agatataatc cagttctttc atcttgtttc catttccatt gttgtatttc ttttatggta
    10801 ttagtgcaac taggatgtac atgtattctt aattgtttca aataatctat ttgagcttta
    10861 acacttcctg gtcctttttt aactccttta gctttatatc ctgcactctt ccacatctta
    10921 attctatctg gttcagcact atcacagtac ataaatagat tcttttctaa acctttactg
    10981 tttgcaatct ttatgatttc agaggtatcc atctcatgta catatatttc attacatata
    11041 tataactctc catccttaaa gccaattcta agtactacat ttgcatggtt aaatccaaag
    11101 tcttgtgata acctcatatt atcaaagtat tcaaattctg taggaaattc atgtataaca
    11161 taatttttaa gtattgctcc accagtttct ccccattctc caagaccata gactttgtac
    11221 ccttctgggt cttgctcttt tctcatttgc attcttctgt agtaagcctc atctatgaat
    11281 ctattttgta gataagtact atgatgagta aatatatcat catttttata gtcaaaatac
    11341 tttcttttta tccaatgagt agctgaaact ggattaaatg taaatgtcat ttgatagtat
    11401 aggttaggat tagttaaaat acctcttaaa cggtcatcta gtatgtctat gtcactttcc
    11461 ataagttctg tagcttcttc acaccaaacc catgttaatt ttcctttcga gaagttaatt
    11521 gattttaatt tttctctttg ttttgcatca ttaactcctc tgaaaattat agagttacca
    11581 gtaactttac tcttaatttc taaaggattt aaagtagttt tccaatactt atcagcttgt
    11641 ttaccataaa tacgatttat agctcctgta agctctgcat acgttgaata cttatgtgta
    11701 gcttctgact ttctaactac taatagatta gctccttgat acttcttatc tcctaacttt
    11761 agtatatagt cttgtgctac attaacagat tttccactcc ctgctgaacc tttcattgct
    11821 ctgtatcttt ttttagtaaa attggcttcc ttgaaatctg gattaaaatt tactctaact
    11881 atcatttcta tcaccataat ctacacttat tttcaactca tcatctccaa tatcatcttt
    11941 acttaggtta tcaacttcac atttcaactt ctcaactcta tttttctgct cctctgtagc
    12001 caaattccaa tccttatgaa tcatttcatc atactgttta attaaacttc taagttcact
    12061 catagcccta ctctgtgcat taagaaaaga tgcttgccta tcccatgcaa attgaaattc
    12121 atactctatc ttctcaccat tttctgtgct ttcatatttc tttaattctt taaccatttc
    12181 ttccttgtct ttaacataca ttatcttttg tgctcttatt attgctgcat actgaattgt
    12241 tatctgctcc caaagaatat caaatttatc ttttgtggat atttcttgta tcaattctct
    12301 agtttctttg ggtagatact ttgagaagaa accaaacttt tcagcatttt tattacctgg
    12361 tggaccagta gcatttttat tacctatggg tgcacccctt ttatttttag gtgcaccctt
    12421 ctttttctca ctagaccaat tgtatctttt tatccatgac tttaaagtgt ttaaactaat
    12481 gtcatacttt gctgatattt ccttttgttt catacctttt atgtaatctt gttttacctt
    12541 ttctttcaca tcttgcacac caccacctcg tttgtttgtc gttttgggaa taaaaaaaag
    12601 aactcatttg gagttcttta actaattttt attattttat taatcttatt gtttcattta
    12661 aatcatcatc tgatggaaca gattttttaa ttttatttct tacaatctta attataaacc
    12721 aaacatctct tgtggcttta aaaaacacta ttagtgttaa taacaaactc aacaattcaa
    12781 tacttggaac tactatataa aa
//
LOCUS       DAGSHT010000099        12640 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGSHT010000099 DAGSHT010000000
VERSION     DAGSHT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.101"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(68..1324)
                     /locus_tag="MJB72_002343"
     CDS             complement(68..1324)
                     /locus_tag="MJB72_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY8263433.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            complement(1373..3037)
                     /locus_tag="MJB72_002344"
     CDS             complement(1373..3037)
                     /locus_tag="MJB72_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY8263434.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            complement(3027..3875)
                     /locus_tag="MJB72_002345"
     CDS             complement(3027..3875)
                     /locus_tag="MJB72_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8263435.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(4105..5157)
                     /locus_tag="MJB72_002346"
     CDS             complement(4105..5157)
                     /locus_tag="MJB72_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8263436.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(5157..5831)
                     /locus_tag="MJB72_002347"
     CDS             complement(5157..5831)
                     /locus_tag="MJB72_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263437.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            complement(5936..6772)
                     /locus_tag="MJB72_002348"
     CDS             complement(5936..6772)
                     /locus_tag="MJB72_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263438.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(6774..7817)
                     /locus_tag="MJB72_002349"
     CDS             complement(6774..7817)
                     /locus_tag="MJB72_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263439.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(7836..8375)
                     /locus_tag="MJB72_002350"
     CDS             complement(7836..8375)
                     /locus_tag="MJB72_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263440.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(8811..9500)
                     /locus_tag="MJB72_002351"
     CDS             complement(8811..9500)
                     /locus_tag="MJB72_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263441.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            complement(9951..11858)
                     /locus_tag="MJB72_002352"
     CDS             complement(9951..11858)
                     /locus_tag="MJB72_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBY8263442.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            complement(11875..12561)
                     /locus_tag="MJB72_002353"
     CDS             complement(11875..12561)
                     /locus_tag="MJB72_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBY8263443.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
BASE COUNT         4173 a         2219 c         1229 g         5019 t
ORIGIN      
        1 aaaaaacaat actaaagata taattgatat aataaatttt ttcatgttaa ttcctcctgt
       61 acatgaatca aaatctttct gttttatacc atttaacctc tcttttaaaa attatatctt
      121 ttagatactc tatcattcct tttatagcta caaacagcca tagttgagag tatgtaaaat
      181 acattattgc aactatgaat atattttcaa ttgttgcttc acctttttct atagtaaggc
      241 ttatactcac ttctataata aataaaagat aagatagtat ccatattaga aaaaagttaa
      301 ttggaataga aatttctact agtttagata tacttaaaac aaataaaata tcggatataa
      361 ttacagatgt taaaaataga aaatatacag aaaaaaagta agctatatca aacacaatct
      421 tatttcttcc ctgtttaaat atatttttta tatatttcat taatacatat atattccctt
      481 ttgcccatct agtacgttgc tttatccaca ccttaacagt ttctggctct tgttcccatg
      541 ttatagattg tggtacaagc tttatcttgt atcccaattt atatatccta aaacttattt
      601 ctgtatcttc tgcaattgct ttactatccc atcctccaat ttcttctatg atacttctcc
      661 tcaatataaa gttagttcct ggtattgtac atagattaaa aagttgccat ctaccagctt
      721 gtgacatcca ttgaaagctt aatgtttcta tatttataaa tttggttaat aggtttttat
      781 ttttatttcg agtcctaaat ttcccaataa cagcacctaa ttcatcattc attactatag
      841 tttgtactag atatctaagt gcatttttat ctggtgtgtt atctgcatca taaacagcta
      901 taaaatctcc ttttgaaatc gtatatccaa tatttaatgc attagacttt ccttttccac
      961 ctgttaaact atctgtattt attatagtga aattataatt gttgtaccta tcctttatat
     1021 tttctaaaat ttctttacta ttatcagaag aattgtcatt tataacaata agttccatct
     1081 tactcttagg ataattgagt aatagcaaag actccacagt tctacctatc actttagctt
     1141 cattatgagc aggaacaagt attgatatca taggatattc atcaatttct tttatttctt
     1201 tatcaaagtt tttaaaataa aatatatatc cacccatagc taaaataata tttataagca
     1261 ataaagacca tatactaaac agactaaata cgaacaaata ctctgataca tttaaatctc
     1321 tcataaattt ctcctaaaat attttaaaat attgattcta atataaaatc atttatttta
     1381 aaaatttctt tttatctatt ctcttaaaat atataaatat aatcacaaat attaaaacac
     1441 ttatagatac tatgaatatt agaattttac taatattgga tatggattta aatctagtat
     1501 cacttttagt tatttcatct aagtccttat catagttgac tataatttca tctccattat
     1561 ttcttatttg tatttcatca acttttaccc aattattaaa atctcttaaa ttcataaatt
     1621 ctatattctg tttttctaaa tattctattg tgttttttag atactctatg tttaaatatg
     1681 aatgaaagaa gaaccctcca ctaaaacctc ttacaatgga taatttatct aaattttctt
     1741 ttatattttg aaaagtaaac ttatcttctg ggtctatata tcctaaattt tcgggtataa
     1801 atatattaaa ctcctctgta tccctaataa tataaggata tgtatttgtg ctaaattttt
     1861 tatcattatt ttgatgttgc cccatataag tagaaaaata tttttttaat tctttatatc
     1921 cctctgattc cattgcataa tgtggcgctt caaatgcaag aggataaatt ccattctcta
     1981 tacatactct aagaccattc aaaactctat ctttgacaaa tatcttcata ttttctttta
     2041 agggttcatc ttttttacca tcccaaaatt catacccttc tccagaaact tcttctttct
     2101 tgtattgatg agtatatcca tgcaatatca cagttccacc tttatcttgc atatatttta
     2161 ttgctttgac aatttcaggg ctttctgata aagttattac tttatgattt ttaggattta
     2221 catatgctgg tattaatgca atcgtaaatg gtatattctt actacttaaa taatccgcta
     2281 tctctacaag attttttggt tctctaaaag catgtacatc ttctattctt acaaatatac
     2341 gtcctttatc aaacgtctta atattaaaaa tatcattaag tgaatcgcaa aatatataaa
     2401 ataaaactcc atctaaatct aattttgata cataaaaaag atttttatca tttattatat
     2461 atggatactt atttaaggta tcattaatac ttcctattac tttattactt gtatcttttg
     2521 attctacaat gttaaaaaca taatgttcat ccaagttata tgatttacct ctataattta
     2581 cactaactat attgtttgtt tttcctacat agtctaaatt gtatttttta tgttccaaaa
     2641 gattttctat accatatcct aaccaacata tagttccttt atatgaattt aaggaactaa
     2701 tcaagttttt agtgttttta tcattattat atgagttttc attgattcct aaaataaata
     2761 tatgactgta ataattttta tttatctcac catcatagtt actcaatttt aatagctcta
     2821 tatctgaact aaatcttccc aatagagtcc tcataatatt taaaatatct ctattataag
     2881 ctgtttcttt cttagaatca tatatgatta ataccttatc tgtgctggaa gtaatttctg
     2941 atgcatatga agataagcta aaatttgata ttgtcagtac tattgataat acaactacta
     3001 attttaatag acttttatta aatattttaa acatcatatt gaagttcctc ttcaactata
     3061 ttcttaaaat ttatactatc tccaaaacta ctcttatatt gtaaaaatgc aattttgaca
     3121 tctatattta catattttcc cctattgttt aaatctaaat ttaattcttt tatccttttc
     3181 tttattctgt ctttaacaac ttttgaacct tccaaagatg tatttggcat tagtatccct
     3241 atcatatctt tttgtaaact ataaattata tcttcatttc ttgtacattc tataatattt
     3301 gaaccaatat attttactat tttattagtt ttgttttcac caaatatagt ctgtaggttt
     3361 ccgtagtatg gtaattttac tatcataaga gaaaaatcag tattatgtct aatagattta
     3421 cttatctcca tatttacatc attataaaaa atctttaaat ttctaagtcc agtttcacta
     3481 tcaatagtca ctaattcctt atactgttca gatagttttt tatttgtatt ttgcaattca
     3541 tttatgcttt tgttcagatt acccattata attgaagata ctggtgttgc tattatccac
     3601 atatatgata taaattcaac atccatattg tgagtaatat tgttatataa aatataggat
     3661 gtatacataa atataattat tgaacttgtt atcaacccca ctactgaatt tgataaataa
     3721 gttaacatta ttataaaaaa aattattgct aacattatga acacattcaa tttattttct
     3781 gtcctaatcg atagtattac tattccaaca attaaaaata taaaaacgag caaagccaat
     3841 attaataaat ctattttttt atttatccta ttcatttttt ataaatttac acctccaatt
     3901 attctattaa gttgcccatt ttttctaatt attattaaag tcattataca aataagtttt
     3961 catttattaa taaactattt aaattgcata tgaatataac ttaaacaaaa aataaaactg
     4021 ttcaataatt agaaaataaa ttttctaaaa aagaacagtt ttattaagta tttattaagt
     4081 aaatttactt aaaacaacaa gaccctattt tgattttaac tctatccaag ccttgtcata
     4141 taattttata ttttcaccaa tatcttcaaa agtttcacat ttatcaagta cttctttaga
     4201 tggatatgca acaggattct gagttatttc tgggtctaat aattttagtg ctcctgtatt
     4261 aggtgttgaa tacccaatgt actcagcatt tacttttgca ttctctgggt ctaataaaaa
     4321 gtttataaac agctcagctt ctttcttatt ttgtgctgtt gtaggtacac acatagcatc
     4381 tacccattta ttagttccct ctttaggtat agcataatca aggtcaggat tttgctccat
     4441 tagtgtaact gcatctccag aatacagcat acccattgcc acttctccac caagcaatct
     4501 atccttacct tcatcattta cataagccaa tactaaatcc ttttgtttca ttaaaagatt
     4561 tttagcttca tttatttctt ttggatttac actattcatg ctatatccta attttttaag
     4621 agttatccct atagtatctc taacagagtc aaacatcatt atatttccct tatactttgg
     4681 attccaaagt atattccagc tatccatagg gtctttaact tctttcttat tgtacaatat
     4741 tccaagagtt ccccacatat atgcaacact atattcatta gttttgtcgt atacaggatt
     4801 taaaaaactc ttatctaaat atttcatatt aggtatattc ttaaaatcta atttttccaa
     4861 aagaccttca tttttcattt tctctaccat atagtctgaa ggaaatacta agtcatagtg
     4921 tgtactacca ctctttactt tttgatacat catttcatta gtatcataag ttgaatattg
     4981 aacatctatt cctgtttctt tttcaaactt atctataaga gactcatcta tataatctcc
     5041 aacattatat acatttaaaa cttttgtaga atcagttgct ttattacatc ctgtaaataa
     5101 agtaactgtc atcataccta cagctataag ggatattgct tttttaaact taatcattat
     5161 ttaatccccc ttactataga ttctttttta ttagctagta aaagtaaacc tagaaccact
     5221 gcaaacatta ttgttgataa tgcatttatt tcaggtttta tacctcttct agccattcca
     5281 tagatttcta tagaaagatt gcttacacca tttccagcat taaagaaact tattataaag
     5341 tcatctattg acatagtaaa tgcaattaaa aatccagaaa ctatacctgg ttttatttgt
     5401 ggtaaaatta cttttctaag agcgtatata ggtgttgctc ccaagtccat agctgcatct
     5461 tctatattat caggtaattg cttcattttt ggcaaaactg aaagtataac gtatggcaaa
     5521 caaaacatta tatgtgccaa tagcattgtt gtaaatccaa attccatttt tacaaataca
     5581 aacagactca taagagctac tgctgtaact atctctgtat ttaaaatagg aagatagttt
     5641 acatttagta ttaatttctt actcttacct ctcatcttat gtattcctat agcacttata
     5701 gttcctacta ttgtagatat tacagacgct aatatagcta ttacaatagt ataatataaa
     5761 gcactcatta tactctcatt ttgaagtaat tgaccatacc acttccatgt aaatccattc
     5821 catcttgcca ttgattttga atcattgaaa gaaaatagta ccaaagcaaa tatgggtgca
     5881 tacaaaaata agaatactaa tgctaaatat atattagaag ttattttctt taattttaaa
     5941 atagtaaccc accgccttct ttgtcagatt catctccaaa ttttgacatt atagacatag
     6001 atattaatat tactatcatc ataaatatcg atatagctga accaaagttc caatctccaa
     6061 caactgtaaa ctgttgttca atcaaatctc caaccagcat tattttacct ccacctaaca
     6121 gtcttgatat tgcaaaagta gtgactgctg gcataaatac catagttatt ccagacatta
     6181 cacctggcaa actcaaagga aatataattt ttctaaaaac tgtcatatta tttgcaccca
     6241 agtcatgagc agcgtttatc aaatcattat ccatctttga tagtgctgta tatattggaa
     6301 gaaccataaa tggtaaaaag ttatacacca tacctaaaag tatagcaaaa ttagtataca
     6361 aaattgctaa tggttgtatt ccaaaccaac ctaaaaatgt atttaaaagt ccattttttc
     6421 ctaatatagc aacccatgca tatgttctta aaagaaagtt catccacata ggaagaataa
     6481 acaataatat taatgaacct cttctactta cacgagcctt tgaaattata tatgccactg
     6541 gataaccaac tataaggcat attaatgtag ctcctcctgc taaaagaata gaacgcccaa
     6601 aaactttcat atatataggg tcaattactt ctttataatt ctcaagtgta aaggcataac
     6661 ctccacctga ttcttttgta aaactaaaaa atactactaa tattaaagga attattacaa
     6721 atatcgcact ccaaactaca tatggatatg ctaaaaaaga ttttcttttc atcttagtta
     6781 ttctcttttc tcattatatg gatatcttct ggatatatat ccatgcctag ttcagtccca
     6841 acctcagcat ttttagtatt atgaagtatc cacaatcttc cattttcatt caattctatc
     6901 tcgtaatgca ctcctttaaa cactgtagat gtaactttcc cctttaacat tccttcttct
     6961 ggctttacca tttttatatc ttctggtctt attactactt ctatattttc gtttttttcg
     7021 aatcctttat caacacactc aaaatctcta ttacagaaat taactttata atcttctatc
     7081 ataattccat caaatatgtt actttcacct ataaatttag ctacaaatga atttttaggt
     7141 tcattgtata tatcttgtgg tgttcccatt tgttgaattt tgcccttata cattacaaca
     7201 atagtatctg acatagtaag agcctcttct tggtcatgag ttacaaaaac aaatgttatt
     7261 cctaattttt tttggattct ctttaattct atttgcatct cttgtcttaa ctttaaatca
     7321 agtgctccaa gtggttcatc tagtaataat acctttggtt cattaactag agctcttgct
     7381 attgcaattc tttgttgttg tcctccactc aatgattcaa cttgtcgtct ttcaaaacct
     7441 tctagtgcaa caagtttcaa aacctctttt actttagcat ctattacatc ttttggagtc
     7501 tttttaattt ttaatccaaa agctacattt tcataaacat ccatgtgtgg aaataaagca
     7561 tatttttgaa atactgtgtt tacttgtctt tcatatggtg gcaaattatt tatctcctta
     7621 ccttcaaata aaactttacc atcatcagca tactcaaatc ctgctattat ttttaatgtt
     7681 gtagtttttc cacatccact tggtccaagt aatgttagaa actcattttt ctttatatct
     7741 aaattgaaat tatctaaaac agtaagttct tcataatgtt tagatatatc ccttaattct
     7801 attatatttt ctaccaatta ttaaacacct ctattctaaa atgatggtgg cgtacttacc
     7861 caaatcactt ttgctgtagt tttgccctcg tttgatatat agtgatttgc tttcggtcta
     7921 aagtaaaaac tctctccttt ttttacttta tttttctttt cgcctatatg gacatatatt
     7981 gaacctgcta atacatatcc aaattcttca ccttcatgtg gcttttcttc tttatactgt
     8041 cctcctggat acaatgttat tattattggc tccatttcat ttttttgtga atttggaatt
     8101 aaccacttta atttatattt taaatcttca tcttcagttt caaacatatc ttcttttgta
     8161 aaactgattc tttcatcatc tatttcatta aaaaattccc ttaaatttgt acctagtatt
     8221 tcaagtatat ctataagggt agctatggat ggtgatgtta agttattttc taattgagaa
     8281 ataaatccct ttgacaattc acatctatta gctaactctt cctgggtcag ttgtttttct
     8341 gttcttaatc tttttatctt ttcacctata tccatgttgc acctcctagt ttctgtttta
     8401 ttttactaaa cattttgttt actaaacatt gagtttaaaa tttctaaaca acataggtaa
     8461 ttatatataa aatttggata taatacaaga gtttttttga cattttttta tttttttatt
     8521 tagaataaat acaagcattt taaaggcttt ggaaaataca cagtttaaag gtgtttatat
     8581 taatatctat aaagtttagt atctctaatt ataaattaat tcattcatta aatagagaat
     8641 aagacttcta gtactaaaaa aatattgaac aaataaaaaa ttgctagaaa tctatatttt
     8701 atagtgttct agcaatcctc atattttaat attcttttaa tattcaattt atctatctaa
     8761 acaaaatgtt aatttaaaat acttaacatt tcgttgaatt tattcttaaa ttacttaact
     8821 atattttgaa cagcagcttt tttctcacca ctcatataca ctttgataaa ttttatatca
     8881 tctactataa atctatgttt aatcttacta caatctaatt taaataagcc aaataccatt
     8941 tgtgtgaaat accccaatcc aaatgcactt attatagttc ctataccaac ttttccacct
     9001 aataaaaatc ctactattaa tgctccaact tctatagtac ctcttattaa tttaagtgat
     9061 tttcctgttt tcttttgaat tgcaaccatc aaaccatctc taggtccact tcctaaacca
     9121 cttcccatat aaaataccat tccaacagac aaaagaacca atcctaatat caacataatt
     9181 attcctacaa ataaattatc agcctctgga attaaattac tatataaaag taaatccata
     9241 aaaaatccaa ttagtagcat gttaaaaaca gttccccatc ctacattttc tccaagaacg
     9301 acatcagcaa taactactat agagcctaca attatagaag cagttcctat agttattcct
     9361 atatgatttg aaagtccttg atgaaataca tcccagggag ataatccaag gttacaattt
     9421 atcgtcaata ctataccaac tgaacatata aataaaccga ctactagctt taaaaatttc
     9481 cataatactt tttgcaacaa attctacccc tcctaaacat aatttaacta ctcactctaa
     9541 atatcaaaag ccagcagttt tcttacaaaa atcttatttt attaatttta taatatactt
     9601 ctatgataca ttattttatg ctaaataact accattttat tacaacttag ttacaatttt
     9661 tgtcattatt ttttggtgaa gtaatttata catttatcca accaatttct atccttcaaa
     9721 tgttttcaat ttcatttata ttatttataa atgaaaaaaa ctgtctaaat ataagttatt
     9781 atacttaaac agttttttat tactttatta tgtattatta atatgctgta tttaattttt
     9841 atcaatttat taccttatat tattaatcaa acacattatt ttctaaaaaa tactgctaag
     9901 taaatattta gacttaacag tattaaaatt atttatattc aattttaatc ttattttgac
     9961 tttataaatc ttcttctctt tacccttgat acaccaacta acataatcga cattattatc
    10021 attgaagctc cacctaaaat taatttgaag tatgttgttg ttttactcac agagcctcct
    10081 cctctattgc caaaacctcc agcttctctt ggattatctt gatttctatc catattcata
    10141 ttgccatttt taccattcat atctccctta tccatattac ccattcctgg tggcatttta
    10201 ccatccattc cctcaggtgg ttctggcata ccactcatat cctcatcaaa cttttcacta
    10261 cctttaattc cacttgaact ttcatcacca gagttttgct ttgtcgaaga agtttctcca
    10321 cttaattgag attttatagt tttactcata tcttctgcaa cttccaaaac ccctggtatt
    10381 gacttacctc ctttacccat aggaccttct agcttttgat tattatcatt tccactttta
    10441 tctttatctg tattattctt actttcacta tcatttgtag ttttattttt agtttcatta
    10501 tctgtattag ctttgactac gcttgatgtt aattcagcat taacttcttt ttcatcactt
    10561 tgatttccag agcgcttatt ttcactatta gaattattat tagaatcact gttattagag
    10621 ttattgttgt caaacccttt attaccaaat cccttattat cactagaatc ttcaattgaa
    10681 gatgttatat ttttttcaaa ttcttcataa gtataaaatg ctgttgggtc ttcttttaca
    10741 tatgatgcta tcatgtcatg caattttgtt gtcatattct ctaaatagtc tgaatctagg
    10801 tattttgtta ctatctcttc cagatattta tggtattttg ttttgtatgt ctcatttttt
    10861 aataacgagg atatgagagg tctgtcttct aaattacctg tcgtaggttc atcaattgct
    10921 atagattgac tacctccacc aaaaccacta aatccaccaa atgacatatt aaaatcccat
    10981 ggtaacatag aaaatactcc atcttgctca tataaataat agttatgggc aaaactccct
    11041 tgatagctat caaggtttaa taaagctgta tttattgcta tatttttaag gacagaatct
    11101 acatcaagat atttttcaat atcttcacct gtatccaaag attttaatag ttttgtaatt
    11161 ttagaccaat cagctgtctt tttatcactt tcaacgatta agtttgagta actttctggg
    11221 tcatctcctt tatattgcaa cgagcttcct tcatctgact tatataagtc tccagttaca
    11281 ttaccaaaat tattttcaag ataagactct tttaatcctt ctactgccaa atacaaacca
    11341 tgatattcgc catttataga gactttagca tatgcaaatt ctggagtcgc taatcccatt
    11401 tcctcgcaaa tactatatgt taaaaactct ctcatataag atgggtcaga gtaacagtta
    11461 ttaagattta attgagtaag cccttccata ctttgactag tattatactt atcaaaatta
    11521 atcttatagc tgtatctatc actatcacta tttgctacag atataagact tgaatttcct
    11581 ttagttctta tacctacgtt tccatatgta tctccatcta cagttacttt tgcaacctta
    11641 aattcttctt ttatagcatt ttcattcata tccttcaagt cactttcatc tatctctata
    11701 ttaacttcca taaccttgtc tctgttaaaa tatttttcaa tatatacatc actatataaa
    11761 tcagcacttt tgttgctaga tgtactataa actccaacta cagcacataa aaatataatc
    11821 ataatcgaga taagaagggt aaatttttta tctttcataa aagattactc ctctctatgc
    11881 tacatagtca ccattataac ttacaagaac tgcattagta actccatcta cttgactcaa
    11941 gtcatttata agacttgttt ctccatcttt cattcttact tcaaacacta attctattcc
    12001 tttttctggt gtaacacttt ttgattttat ttgatattta gtaaatactt ttttaatttt
    12061 atcagttgct atatcttctg aatcttcatt attacaattt accatcaata tatatggagt
    12121 ttcagatatt gttttatttg aaaagaatat tagaatcaat cccataatta ttgaacccat
    12181 gacagctaag ggaatcaaac ctgctcctaa aactatacct gaacctaatg accaaaatag
    12241 aaatactaaa tccattgggt ctttaattgc tgttctaaat cttactatag ataaagcacc
    12301 aaccatacct aaggataaaa ctacatttga agtaactgcc aatattacaa aagttgtaag
    12361 cattgtaagt gctacaagcg acacattgaa tggtcgagaa tacataattc ccatataagt
    12421 ctttttatag accatatata taaacaaacc tactaaaaaa gctgaaccta gtgctaaagc
    12481 actgtccaca aatgaaaaac ctgatacgtt ttctataaaa cttgatttaa aaatatcatt
    12541 aaatgtcata attaaaatca tccttcctat ttttaactta tttatttttt attttattta
    12601 tttttaactt atttgttttt taaccttatt tatgtatatc
//
LOCUS       DAGSHT010000100        12072 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGSHT010000100 DAGSHT010000000
VERSION     DAGSHT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.102"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..178
                     /locus_tag="MJB72_002354"
     CDS             <1..178
                     /locus_tag="MJB72_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY8263444.1"
                     /translation="IAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIERKSSEN
                     GTEVLITIPTQINI"
     gene            362..1795
                     /gene="eutA"
                     /locus_tag="MJB72_002355"
     CDS             362..1795
                     /gene="eutA"
                     /locus_tag="MJB72_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBY8263445.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            1813..3177
                     /locus_tag="MJB72_002356"
     CDS             1813..3177
                     /locus_tag="MJB72_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBY8263446.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            3190..4071
                     /gene="eutC"
                     /locus_tag="MJB72_002357"
     CDS             3190..4071
                     /gene="eutC"
                     /locus_tag="MJB72_002357"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBY8263447.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNAYI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKGNNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            4091..4744
                     /gene="eutL"
                     /locus_tag="MJB72_002358"
     CDS             4091..4744
                     /gene="eutL"
                     /locus_tag="MJB72_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBY8263448.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            4755..5456
                     /locus_tag="MJB72_002359"
     CDS             4755..5456
                     /locus_tag="MJB72_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY8263449.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGNTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            5459..6928
                     /locus_tag="MJB72_002360"
     CDS             5459..6928
                     /locus_tag="MJB72_002360"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBY8263450.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKSVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            7024..7311
                     /gene="eutM"
                     /locus_tag="MJB72_002361"
     CDS             7024..7311
                     /gene="eutM"
                     /locus_tag="MJB72_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBY8263451.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            7438..8199
                     /locus_tag="MJB72_002362"
     CDS             7438..8199
                     /locus_tag="MJB72_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HBY8263452.1"
                     /translation="MGVLTESEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            8212..8841
                     /locus_tag="MJB72_002363"
     CDS             8212..8841
                     /locus_tag="MJB72_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBY8263453.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            8883..9608
                     /locus_tag="MJB72_002364"
     CDS             8883..9608
                     /locus_tag="MJB72_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBY8263454.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            9621..9893
                     /locus_tag="MJB72_002365"
     CDS             9621..9893
                     /locus_tag="MJB72_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBY8263455.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            9886..10452
                     /locus_tag="MJB72_002366"
     CDS             9886..10452
                     /locus_tag="MJB72_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY8263456.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVREKEVIYKTVIPSVDSQIIKSLIK"
     gene            10464..11555
                     /gene="eutH"
                     /locus_tag="MJB72_002367"
     CDS             10464..11555
                     /gene="eutH"
                     /locus_tag="MJB72_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBY8263457.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            11557..12030
                     /locus_tag="MJB72_002368"
     CDS             11557..12030
                     /locus_tag="MJB72_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBY8263458.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
BASE COUNT         4875 a         1375 c         2365 g         3457 t
ORIGIN      
        1 tattgctatt atagatgatg gaattggatt tgatagtaat aatataaata aaaatagctt
       61 agggacatat ataatagata gttatataac acagatgtta aaaggaaata tagagagaaa
      121 aagtagtgag aatggtacag aagtattaat tactatacct acacaaatta atatttaaat
      181 aataactcat ctacaaagta gtagttttaa ttgagcaaag aggcttaagg ttattaatag
      241 attaagttaa ttgaatgtat ttataaaaat tataagtata tattcaattt aacaggtatt
      301 tattataact ttaagcctct ttgcttgttt aagacatact taaattttag gaggtgagaa
      361 aatgagagaa gagttattaa gtgttggtat tgacattgga acaagtacta ctcagttaat
      421 tttttctaaa ttaatagtag aaaatactga atcaaatttt tcggtaccta gaataagtat
      481 agtagacaaa gaaattattt ataaaagcaa agtttatttc acccctctta tttcaaataa
      541 tgaaataaat ggtcaacaaa taaaagatat agttgaaaat gaatataaaa aagcaaatat
      601 atctaaagaa gatatacata caggagcagt aataataact ggtgaaacag ctagaaaaga
      661 aaatgcaaat gatgtacttc atactttgag tggatttgct ggggacttcg ttgttgcaac
      721 agcaggacct gatttagaaa gtataatatc aggaaaagga gctggagctc atatttattc
      781 aaaagaacat tcaactacaa ttgtaaatat agacataggt gggggaacta gtaacatagc
      841 ctcatttaaa aggggagaag taaccgatac tggatgtttg gatataggtg gaaggcttgt
      901 aaagatagat aaaataagta aaaaaataac ttatatatca cctaaaatag ataaaatcat
      961 aaaagataat aatttaaatc ttcaagtagg cacaatggct actgtagaaa atacaaagcc
     1021 aatagtggat ataatggttg acttattaat ggaaagcata ggattaaaga aacagacacc
     1081 tttgtttgat tttataataa ccaataaatc aataagtata gatgaaacta ttaaaaatgt
     1141 ttcattctct ggtggagtag ctgattatat ttactatgat ggagaaataa aagattattt
     1201 tgaatatggt gacttgggaa ttttacttgg gcaagcaata aaaaattgtg aataccttaa
     1261 aaaagtaaat gtagttaaat ctatggaaac aataagagcc acagtggttg gagcaggttc
     1321 tcatactact gaagtaagtg gaagtacaat aacttacact agcgaaaagt ttccacttaa
     1381 gaacttgcca gttttaaaat tatcaattga tgaagaaaca ggtacttcaa aacaaatcag
     1441 tgaagctata aaaaataaac ttaattggtt taaactagaa aatgatttac agaaagttgc
     1501 tatagccatg ataggaaaga aaaatccaac ctttttagat atacaagagt atgctaaagg
     1561 gtttgtagat ggaatgcaac aaatcataga aaatgattta ccttttatag taattgttga
     1621 aaatgatatg gcaaaagtgt taggtcaagc aatatatgca ttattaaatt atgaaaaaga
     1681 aattgtttgt atagacagtg tcaaggttga aaatggagac tatatcgaca ttggaaaacc
     1741 tatagttgaa ggtaaagttt tacctgtggt aataaaaaca ttagttttta attaatagaa
     1801 ggaggtaagt aaatgatttt aaatacaaaa ttatttggac gtacttatca gtttaaaggc
     1861 ttatatgatg taatggctaa agctaatgaa gaaaaatcag gagataaatt agcaggtctt
     1921 gcagcagaat cagctgaaga aagaatagca gcaaaagttg ttttatcaca cataaaattg
     1981 gaggatataa gaaataatcc tgcagtggca tatgaagaag atgaagtaac aagaataata
     2041 caagatgctg taaatgaaaa agtttatgaa gaaataaaaa attggacagt ctctgaactt
     2101 agagaatggc tattggattc aaatactaca agtcttgata taagaagaat atcaagagga
     2161 ttaacttctg aaatgatagc agcagttgca aaattaatgt caaatatgga cttagtttat
     2221 gcagctaaaa agataaaggt tactgctcac tgtaatacaa ctataggaga acaaggaaca
     2281 ttatcagcaa gacttcaacc taatcatcca acagacgacc cagatggaat acttgcatca
     2341 cttcttgaag gtttaacttt tggtatagga gatgcagtac ttggattaaa tccagttgat
     2401 gactctgttg agagtgttac taaagtatta aaaagatttg aagaagtaaa acaaaaatat
     2461 caaataccaa ctcaaacatg tgtattggca cacgttacta cacaaatgga agcaataaga
     2521 aatggtgctc ctgcagattt aatattccaa tctatagcag gttctgaaaa aggaaatgaa
     2581 gcatttggat ttaacggcga cactatagaa gaagctagac agttagcttt aaaacacggt
     2641 acagctacag gacctaacgt tatgtatttt gaaactggac aaggttcaga attatcttca
     2701 gaagcacatc atggaataga ccaagttact atggaagcta gatgctatgg atttgctaaa
     2761 aagtatgaac cattcttagt aaatacagtt gttgggttta taggacctga gtacttatat
     2821 gatgctagag aagttgtaag agcaggactt gaagaccact ttatgggtaa actacatggc
     2881 ttaccaatgg gtgttgatgt ttgttacact aaccatatga aggctgacca aaatgatatg
     2941 gaaaacttag ccatactatt aacaacagca ggatgtacgt attttatggg aatacctcat
     3001 ggagatgatg ttatgcttaa ctatcaaaca acaggatacc atgaaacagc atcacttaga
     3061 gaaatgtttg gactaacagc aataaaagaa tttgatgctt ggatggaaaa gatgggattc
     3121 tctaaagatg gtaagttaac agagttggcg ggagacgcat ctgtattatt aggataggag
     3181 gtatttaata tgaatgagaa ggatttaaaa gcattggtag aacaattagt tggacaaatg
     3241 gttggagaat tagatactaa tgttgtgtct gagactgtta aaaaggcaac tgaagttgta
     3301 gtagacaaca atgcttatat agatgatata acagaagttg atataagaaa gcaattacta
     3361 gtaaaaaatc ctaaggatgc agaagcttat ttagatatga aagcaaaaac acctgcaaga
     3421 ttaggtatag gaagagctgg tactagatat aaaacagaaa cagttttaag atttagagca
     3481 gaccatgctg ctgcacaaga tgctgtattc tcttatgtag atgaagaatt tataaaagga
     3541 aataatatgt ttgcagtgga aactttatgt aaagataaag atgaatactt aacaagacca
     3601 gatttgggaa gaaaattttc tccagaaact ataaataata taaaatctaa atttggtaca
     3661 aatcaaaagg ttttaatatt agttggagat gggcttagct cagctgcaat agaggcaaat
     3721 ttaaaagact gtgtgccagc tataaaacag ggtctaaaaa tgtatggtat agattcaagt
     3781 gaaattttat ttgttaaaca ttgtagagtt ggtgctatgg accacttagg tgaagaatta
     3841 ggttgtgaag ttatatgtat gttggttgga gaaagaccag gattagttac tgctgaatca
     3901 atgtcagcat atatagctta taagccatat ataggaatgg cagaagcaaa aagaacagtt
     3961 atatcaaata ttcacaaagg tggaacaact gcagttgaag caggtgctca tatagctgaa
     4021 cttataaaaa ctatgttaga taaaaaagct tcaggaatag accttaaata aagtcgaggg
     4081 agtgtagaat atgaaaaatg atgtaattcg tcctaatata ctaggaatta aaataatttc
     4141 aaatataagt ccagaaatgg cacaaaaact agaattaaaa tcacatcaca aaagcttagg
     4201 tttaataact gcagactgtg atgatgttac gtatacagct cttgacgaag caactaaagc
     4261 ttcagaagtt gatgttgtat atgcaaaatc aatgtatgca ggtgctgcaa atgcttcaac
     4321 aaaacttgct ggtgaagtat taggaataat agcaggtcca agcccagcag aagtaagaag
     4381 tggtctaaat gcagtagttg attttctaga gtatggagca acttttataa gtgcaaatga
     4441 tgatgattca atagcatact atgctcactg cgtatcaaga acaggtacat acctttcaga
     4501 ggtagcagga ataagagaag gtgaagcatt agcttattta gtagcaccac cccttgaagc
     4561 aatgtatgca ctagatgcag caatgaaagc tgcagatgtt aagatgtgtg aattatttgc
     4621 acctccaact gaaacaaact ttggtggagc attacttaca ggttctcaat cggcttgtaa
     4681 agcagcatgt gatgcatttg cagaagcagt aaaatcagtt gcagacaatc caacaggatt
     4741 ttaaggatag ttagatggat gctataggct taatagaagt aataggatat gttgctgcaa
     4801 tagaagcttc tgatgcttgt ttaaaatctg caaatgttaa tattgtaaga gttgataagg
     4861 ttggggcagg tattgttact ttaacaatat gtggagatgt cggtgctgta aaagctgctt
     4921 tagaagcagg tgaaatagca gcttccagag ttggtacatt aagaacatca catattatac
     4981 caagaatcca taatgaagta acaaatgctt tattcaaaac taaagaacct aaagtctgtg
     5041 aagtttcaga agataaagaa aaaaactttg agattacatt ggaaataact gataatgtat
     5101 cgaaaaattt atcagaaaaa gaaaatgtaa ttttagatgt caatgaaaat agcggaaata
     5161 ctgaaaattt atatagtgat atagataaaa caaacaaaaa aattgaaaca gttgatgata
     5221 gtttaacaga agctaatgag gaaaaaatag aaaacactaa tgataaagaa gtattggaac
     5281 aagttaaaat agaaaattcc gattttgata gagatgacct atcaaagaac agtgtaaaaa
     5341 aacttaaagc tatggctaag aaattagatt cttcaataac ttataaagat ttaaatgttc
     5401 ttaaaaaaga agaattaata aacttagtta aaaagcttag cagaagggat agatagttat
     5461 gacactttta gataaagatt tagtatctat acaagaagtc agaactcttg taagaagatc
     5521 taaagtggct caagagaaac tttctaaaat gagtcaagag caaatagata aaatagtaaa
     5581 atctgttgta gatgcagcat ttaataatgc tactaaatta gctaaaatgg caaatgaaga
     5641 aactggattt ggaaaatggg aagataaagt aattaaaaat acttttgcat ctatgggagt
     5701 ttatgaaact ttaaagaaca caaaaactat tggaataatt ggtgaagata aagaagccaa
     5761 tacgatagat atagctacac cagttggagt tgttgcaggt cttatacctt caacaaaccc
     5821 aacatcaaca gtaatataca aaactataat atctctaaaa gcaggaaata ctatagtttt
     5881 ctctccacat ccaaatgcaa aaaactgcat acttgaaaca gttaatataa taagtgaggc
     5941 tgctataaag gctggagctc cagaggattc aatattatgt ataactaatc caagtatagt
     6001 tggaaccaat gaattgatga aacataaaga tacaaactta atactagcaa ctggaggaga
     6061 agcaatggtt agagctgctt actcttcagg aacaccagct attggagttg gaccaggaaa
     6121 tggaccagca tttatagata aatcagcaaa tgttaaatta gcaatcaaaa gaataataga
     6181 ttctaaaact tttgataatg gtgttatatg tgcatcagaa caatctatag taatagaaaa
     6241 atctatggaa gaaactgttt taaaagagct taaaaatcaa ggtgcatatg ttttaaatga
     6301 agaggaaagt gaaagattag caaaattcat attaagagct aatggtacta tgaatccaca
     6361 aatagttggt aaaagtgttg aggtaatagc aaatttagca gacttaacaa atgtacctaa
     6421 aaatacaaaa gttctagttg caagagaaaa taaagttggt cacaatgtag cctattcaag
     6481 agaaaaactt acaccaatac tagcgttcta tgttgaagaa aatgtagaag ctattatgaa
     6541 cagatgcaga gaaatattgt taaatgaagg tgcaggtcat acattctcaa tgcatgtaaa
     6601 tgatgaagaa ttggtaaaaa gatttgccct taatatgcca gtatcaagaa tacttgtgaa
     6661 tactccttca gcacttggag gaataggtgc atcaacaaac cttgtaccag cactaacact
     6721 tggttgtggt gcaataggtg gaagttcaac ttcaaacaat ataggaccac ttgatttaat
     6781 aaatgttaaa agagtagctt atggaatcaa agaaatagaa gaacttggaa gagataaatc
     6841 tcaagttgaa aaatcaatgt ttgactcaat ggataaagaa gaattaataa atcttcttgt
     6901 tcaaaaacta gtcaaagaac tagcataact aaaataaaca atgaaactta tagctatcac
     6961 tataaatttc taaaaatata aactataaaa aactataaaa ttaaattttt ggaggataag
     7021 attatggcaa gtgcaaacgc attaggaatg atagaaacta aaggattagt aggagcaata
     7081 gaagcagcag atgcaatggt taaagctgca aatgtacaat tagtaggaaa agagcaagta
     7141 ggtggaggat tagttactgt aatggtaaga ggagatgttg gagctgttaa agctgctaca
     7201 gatgcaggag ctgctgctgc cgagagagtt ggagaactaa tatcagttca tgttatacca
     7261 agaccacact ttgaagtaga tgctatatta ccaaaggtat cagctgagta atataagaat
     7321 aattataaaa tttaaatttt gattgaaaaa gaggctatct caaaaatgag tcttactaat
     7381 aaactcaatg tattttgagg tagtcttttt tatacatcta ataggagatg tgataaaatg
     7441 ggtgttttaa cagaaagcga agtaaaaaga aaaattggaa aaaacaaaaa taatataact
     7501 gaattttata tagaaaaagg tcaaataata actccttctg caaaatctta tttagctgag
     7561 agaagtattg agcttaaata tgttgataaa ctttcagatg taaacaatga acctaaagaa
     7621 gttgtaaaag aagtagttaa agagaaaaaa tatagatata ctactgtatt tggcgctaaa
     7681 ttggatgaaa aaccagaaca tatgactcaa ttaaatggaa atcttttagt ttttaaagac
     7741 cataaaagaa taatattgag aggaaaaata gattctcttg aatcagaaat actaaaggta
     7801 cagatacttt gtcagaaact agatatgcca aagcttgtta aggaattgca agaagtatta
     7861 gactttgtaa gaaatataat gagatgtgaa gttttagatg aaaagctagg ggactttaaa
     7921 cttttaggat taactcctca agaacttaga gaacaatctc actatcctaa aaaatacttt
     7981 ggtataggac atgagttccc aagttatgaa atgggagaag tagtagtagc tataaatagt
     8041 ataagaagta atacacgaga ggttgaactt gcagcctatg aagcatttaa gggtgagtat
     8101 ggaaatgttc acagagaaga tttaataaag gcacttaata gattatcgtc agtattttgg
     8161 ataatgatat ataaggttag aactaataag tataagtagt tggaggacat aatggagaat
     8221 ctattaaatg aaatagtgga agaagtaata actagagtta aaaaagaagc atttatagaa
     8281 gttgaagcat caggaagaca cgttcactta agtagagaag atgtcgataa attatttgga
     8341 gaaggatata ctcttacaaa actaaaagac ctatctcaac caggtcaata tgcatgtaag
     8401 gaaagagtta caataactgg tcctaaagga agtataaaaa atgtagttgt acttggacca
     8461 tgtagaaatg aaactcaggt agaaatatct ttaacagatg gttcaactct tggactaaaa
     8521 gctcctataa aacaaagtgg agacttagaa ggtactttaa gtattaaaat atcaacacaa
     8581 tatggtgaag ttgaacttga taaggggtta atggttgcta aaagacatat acacatgact
     8641 cctaaagatg ctgaaaaatt caatgtatgt gataaagaga tagttgaagc taaggtgatg
     8701 ggtcaaagac ctttaacatt tgatgatgta gtaataagag tgagtgacag ttttaaaact
     8761 tatatgcata tagactatga tgaagccaat gcatgtggtt attcaaaagg aactgttgct
     8821 aaaataataa agaaagcgta aatttaacct actatataaa ttacctctag ggaggattta
     8881 atatgaatta tgataacctt gtagatttaa taacggaaga aatctataaa aaaataaata
     8941 gtaatgaaat aaaaatatct aataaaccta aagctgtgat tgtatttgaa caagataaca
     9001 ataaatttaa cttattaaaa gatgaatttg aaatagtaga atttgataaa agtataaggg
     9061 aatgtgaaat agtgatagtt tcaagacttt gtatgagggg gctatcgaat atagcacttg
     9121 gaaattcaac aagtgatgaa gaaagattta tactaaaaat gattatgaag ggcaaagaag
     9181 tatatgtcct agatgaagga attgaataca aaaaatataa ggatactgca ccaaaagcat
     9241 tatataagaa atttatgtca tttgaagatg aaatatgtaa atttggtgta gaaattataa
     9301 aagacttaaa tgttataact aaaaataagt taaatataaa gaaagaacta gaaagtacta
     9361 gaggttctaa aaaagaagat acaaaagttt tagataaaga tgttttaaat ttaaccagtg
     9421 tagaaagtgt aaaaatagat aatgaattta gtttagattt aaggaataaa aaacttatat
     9481 cggaagctga cttaagaaaa ccaactataa atggagttaa aaacatatta gttaataaaa
     9541 aaagtataat aacaccatta gctgtagatt ttatgagaat acaccatctc aaactaaaga
     9601 aagtgtaagg aggtaatttt atggatatag gaaaagttgt aggtaatgtt tgggctacaa
     9661 gaaaagatga aaaattatgt ggccaaaaac ttttagttgt aaaaatttta aaaactaaag
     9721 acacatacaa ggaaggcttc atggttgcag ccgataatgc aggagcagga aatggagact
     9781 tagttcttat atccaaaggt tcctcagcta gagagtcgat tgaaaaatct catgttccgg
     9841 tagatgcaac tatagttgga gtagtcgatt cgtttgaggt gtttgatgaa taaaagtata
     9901 ggagctatag agtttaaaag cattgctaaa ggtatagaag tatccaatga aatgataaag
     9961 aaatcttcag ttgatgtatt gtacttaaaa agtatttgcc caggtaaatt tctaataata
    10021 gtaggtggag aaacttctta tataaatgaa tgtgtagatt atggaataaa acttggagaa
    10081 ggatatattg tagataactt cgtaataaat gcaatttcac aagaaatatt agatggattt
    10141 aaaaataagt atcaaaagct agatagtata gtctctatag gcgttgttga aagtagtaaa
    10201 gtatgcgctg gaataaaaat gcttgataag actttaaaat ctggcgactt agtattagta
    10261 aaacttcaat tatcttttgc tataggtggc aaattagtat atatagtagc aggagattta
    10321 agtagtttag aatatgcatt aaaagaaagt gaaaatgtag taagagaaaa agaagtaaga
    10381 gaaaaagaag taatatataa gactgtaata ccatcagtag acagtcaaat aataaaaagt
    10441 ctaataaaat aatgggggga attatgagta taaacgaaat tataatctat gtaatggtag
    10501 tcttcatggt acttggagct atagataaat gtataggtaa caaatttggt cttggagaac
    10561 aatttgaaga aggtataatg gcaatggggt cacttgcagt tgcaatggtt ggtgttatat
    10621 gtttagcacc agttcttgct gatgtattaa gaccaattgt agtaccagta tttaatatac
    10681 ttggagctga cccagctatg tttgctggaa gtttattagc taatgatatg ggtggtgcac
    10741 cacttgcact tgaacttgcg aaagacccta atgctgggtt atttggtgga ttaatagtag
    10801 gggctatgat gggacctaca atagtattta taataccagt tgcgcttgga ataattgaaa
    10861 aagaagacca aaaattctta gcaacaggaa tacttgcagg tataataaca atacctattg
    10921 gagcattcgt tggtggatta gttgcaggat tcgaggtaat gatggttctt agaaacttag
    10981 tgccaataat aatatttgca gtagttatag cacttggatt attaaaattc gaaaatgcta
    11041 tgataaaagg attcacatac tttggaaaag gtgtagtaat agttataact ttaggacttg
    11101 ctgctgccat agttgaagct ttaacaggaa tagttgtaat acctggaatg aaaccaatca
    11161 gtgaaggtat agaaatagtt ggagatatag caatagtttt agcaggagca ttcccattag
    11221 tgtttgttat aacaaaggta tttaataaac cattaatggc tttaggaaaa gttcttggaa
    11281 tgaatgatgt atcagcagca ggccttgttg caagtttagc taactgtata ccaatgtttg
    11341 gtatgatgaa agatatggac aatagaggaa aaataataaa tgtagcattc tctgtatcag
    11401 cagcattcgt atttggagac cacttaggat ttacggcagg atttaattct aatatgataa
    11461 caccaatgat agttgctaaa ttagttggtg gtataacagc agtattgtta gctatggtta
    11521 tcgcaaataa gacactaaag aaggagaatg tataagatgg atatatcaaa tatagataaa
    11581 aatttaattg aaacattagt aagacaaata atagaagaaa aaataagtgg aactaaagac
    11641 acagtagatt ttgttagaaa taaagacatt agtggaataa cttctataaa gttaccaact
    11701 gtaaaagtta gtgaatctga tagattagat acagggaatc caagtgatgt agtttacaca
    11761 aaagatttat ttacattaga ggaaagccca agattaggtt gtggtatgat ggaaatgaaa
    11821 gaaacaactt ttgattggac tctaaactat gatgaaatag attatgtaat agatggaact
    11881 ttagatataa taatagatgg aagaaaggtt tctgcttctt caggtgaatt aatatttata
    11941 ccaaaaggaa gtaagataca attttctgta cctgattatg ctagatttat atatgtaaca
    12001 tatccggcag attgggcatc tcaaaattaa taaaaatcaa gttaagttaa taataatcaa
    12061 ataaataatt ta
//
LOCUS       DAGSHT010000101        12050 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGSHT010000101 DAGSHT010000000
VERSION     DAGSHT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.103"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1730
                     /locus_tag="MJB72_002369"
     CDS             <1..1730
                     /locus_tag="MJB72_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8263459.1"
                     /translation="NVLIVNDSDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIA
                     ITPGVPEKVPSFISDIFDLGHRYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLD
                     TGIKDKYKELFLKIEELDTILNLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKY
                     IEDIFVDSLKVLLSEKEILDKVVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKK
                     LEQNLTKKLREKGQIAKYTFKDIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKE
                     LIAQSIHNNSNRKNQPFIAVNCAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELAN
                     NGTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDN
                     SQFREDLYYRLNTIPINIPPLRERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGN
                     IRELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNK
                     ESDVGIGRGLILKALLDKNLQITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDF
                     YNKLKYKGL"
     gene            2099..3319
                     /locus_tag="MJB72_002370"
     CDS             2099..3319
                     /locus_tag="MJB72_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8263460.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3356..4705
                     /locus_tag="MJB72_002371"
     CDS             3356..4705
                     /locus_tag="MJB72_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY8263461.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            4819..5381
                     /locus_tag="MJB72_002372"
                     /pseudo
     CDS             4819..5381
                     /locus_tag="MJB72_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5489..7822)
                     /locus_tag="MJB72_002373"
     CDS             complement(5489..7822)
                     /locus_tag="MJB72_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8263462.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(7819..8502)
                     /locus_tag="MJB72_002374"
     CDS             complement(7819..8502)
                     /locus_tag="MJB72_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263463.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(8573..9814)
                     /locus_tag="MJB72_002375"
     CDS             complement(8573..9814)
                     /locus_tag="MJB72_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263464.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(9825..10511)
                     /locus_tag="MJB72_002376"
     CDS             complement(9825..10511)
                     /locus_tag="MJB72_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263465.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
     gene            complement(10748..11092)
                     /locus_tag="MJB72_002377"
     CDS             complement(10748..11092)
                     /locus_tag="MJB72_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263466.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            complement(11423..>12050)
                     /locus_tag="MJB72_002378"
     CDS             complement(11423..>12050)
                     /locus_tag="MJB72_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263467.1"
                     /translation="TLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDK
                     YVESELNKITEKTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTI
                     VTSINSRSREFALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPF
                     ESINLINWKFIGAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
BASE COUNT         4396 a         1728 c         1504 g         4422 t
ORIGIN      
        1 caaatgttct aatcgtaaat gattctgatg aaactactct agaaactata agtttatttt
       61 ataaaattgg agtgactaat attagaccaa ttccatatat gaatgataat aactataaaa
      121 atataaaaat agctataact ccaggagttc cagaaaaagt acctagcttt atctcagata
      181 tatttgattt aggtcacaga tatatagata tttctacatt tatagaaata ataaatttat
      241 tacaaattga ttcaaaggaa atacaatcta atctagttaa atactcagaa gaaataataa
      301 gtttagatac tggtataaaa gataaatata aggaattatt tttaaaaata gaagaattag
      361 atacaatatt gaatttatca aaagatggta tattatttac ttcaaaagat ggtgaaataa
      421 atacatataa tagtaaagta aaagatattt tagacataaa tgaggatata tatggaaaat
      481 acattgagga tatatttgta gatagtttaa aagttttatt gagtgagaaa gagatactag
      541 ataaagttgt cgtattcaat aagaaatata taaatgtaaa taaaaaaaac atatacaata
      601 gagatgaaaa aatgggcaca tattatagtt tacaagaaat aacatacata aaaaaattag
      661 aacaaaatct cactaaaaaa ttaagagaaa aaggacaaat agcaaaatat acatttaagg
      721 atataaaaac aaatagtcct aaaatgtttg aatgtataga tttagctaag aaagtttcta
      781 aatctgattt aagcatactt atcagaggtg aaagtggaac tggtaaagaa ctcatagctc
      841 agtctataca taacaactca aatagaaaaa atcaaccctt tatagcagta aattgtgcag
      901 ccgttcctga aaatttatta gaaagccagc tattcggata tgacaaggga acttttactg
      961 gaggtctaaa agatggaaaa caaggattat ttgaacttgc taataatgga acaatattct
     1021 tagatgaaat aggcgatatg cctttagaat tacaaacaaa actacttaga gttcttcaag
     1081 agaaacagat aatgcctgtt ggttctcaca atgtgattaa tatagatgtt agaatcatat
     1141 ctgctacaaa taagaattta gaacaaatga ttgataatag ccaatttaga gaggatttat
     1201 attatcgatt aaatacaatt ccaataaata tacctcctct aagagaaaga aaagaagaca
     1261 tattaattat tatggaagat ttaattaata agaaattagt aatcacacca gaagcaaaaa
     1321 aacttataca aaactatatg tggaaaggta atataagaga actccaaaat gttacatcct
     1381 atttaaacat tatgtgtgaa gatattgtat tagagaaaga tttacctcca aacttaaggt
     1441 cttcagataa caaaaataca tcattaaaac taaaatatag taagaatgat atattgaata
     1501 tattagagat actaatttta aataaggaat cagatgttgg tattggtaga ggattaattt
     1561 taaaagcact actcgataag aaccttcaaa ttacagaagg aaaaattaaa aaaatatttg
     1621 aatatctaaa aaaagaagaa cttataattt gtagttcagg aagatatgga agtaaaataa
     1681 ctcaaaaagg agaagatttt tacaataaat taaaatataa aggtctataa aattataatt
     1741 tatcaaacaa agaaatctat tcaggattaa agtactcctt caataggttt ttttattttt
     1801 caagtaccct aaaattataa tttattttgt acataaataa taggaattaa taggaatagg
     1861 ataatttttt tatttcctat tttttttata taaactagca tataaaatat attttcttca
     1921 aaataatatt ttttactgaa aattgaaact atgcaatcat tttttatact atacatatag
     1981 ataaatgatt atatttattc aatattctta taaaaaacaa agttctccat cgaaaattat
     2041 ggcataataa ttgcttatta tattaataaa acaataaaaa gcaattggag gaataaaaat
     2101 gcaacaggct ttgaaattaa aatatcaaac caaagaatta ggaaaagatt ttgtaataga
     2161 aagttgtaat tctataaaac catggcttat aaacataaga agagaattac ataaaattcc
     2221 agaattagct cttgaagaaa atcttactaa acagaaggta atttcttatt taaaagaaat
     2281 cggaattgat tatatggaat ttacaaaaca caatggaatt atggcatata ttttaaaaga
     2341 aagtgcagat aaaacgattt gtataagagc agatatggat gctcttccta ttgaagaaga
     2401 aaacaacata ccttataaat caatacattc tggaaaaatg catgcttgtg gccatgatgc
     2461 acatacaacc atgctacttg gagcatgtaa agttttacat tcaataaaag ataaacttaa
     2521 tgtaaatgta aaattcttat ttcaaccagc agaagaaggt tttggaggag caaaattctt
     2581 agttgaagat ggatgtttag aaaatcctaa ggtcgattat atattcggac ttcatgttat
     2641 gccacatatc gaaacaggat ttatcgaaac aaaatatgat acattaaatg ctagtgtaga
     2701 cactataaaa ataagtatca aaggtaaaag agctcatggt gcatatcctg aaaatggtat
     2761 tgatgcaata gttacagctt cacaaattgt tacatcatta caaactataa taagtagaaa
     2821 cttagaacca aataatgctg ctgttttgac tataggtaaa atatatggtg gtgatgcaca
     2881 caatgtaata tgtgaagatg taaaactaga aggcacttta agaaccttaa atagcaaaac
     2941 aagaaacttt atgattgata aaatagctaa aattgttgga gacactgcat ctgcatttgg
     3001 ttgtgttggg actttacatg taagtgatga aaactatcct gctgttataa atgaaaaaga
     3061 attagtagat acagttatat caaatactaa agaattactt ggagaggaaa aatttatatt
     3121 gagaccaaac ccgtctttag gtggtgaaga cttttctttt tacacagaac attgtaaagg
     3181 agcattcttc catttaggat gtaaaaatga ggaaaagggc ttaatttcac ctcttcatac
     3241 ctctagcttt aatatagatg aagattgttt accaataggt gttatgatgc atgtaatgaa
     3301 tactttgtat tttaattagt acagtaatta ataaaagaat aggagataaa ttaatatgga
     3361 aaaagctaag aaattaggtc tatttagtat gatattatta gggattaata gcataatagg
     3421 ttcaggtata ttcttactac caggtaaagt ttataatcta gcaggtcaaa acagtatgtt
     3481 tatatacatt tttgctacac tcttagttct atcaatactt ttatgttttg cagaagttgg
     3541 aagcatgttt gataagaatg gaggagcata tctatactcc aaaaaagctt ttggagattt
     3601 tataggattt gaagttggta caatgtcttg ggttattaga attatatcat ggtctactct
     3661 agcagtagga tttgcaacag ctttaggttc attttggcca gaatctgcca ctgaatacaa
     3721 aggttatata gctgcaatac tcgtaacact actttcaata aatagcttat ttggaattaa
     3781 aagcacaaag ataatgaata atgtaataac tatagctaaa ttagtaccat taatagtttt
     3841 tataatagtt ggaatattct ttataaaatt tgtaaatatt gttccttcag gaaatgtagt
     3901 taactctagt atgggtccag ccattatatt agtattttac gcatttaccg gctttgaatc
     3961 atttattgtg gcaagtggag aaatggaaaa tcctaaaaaa aatcttccag tcgcattaat
     4021 aaccacaata tttatatgtg caataattta tatattaatt caaattgtct gtatgggtat
     4081 acttggagat agactatttg aaaacagtat tccaattgca gatgcttcaa gtgtattttt
     4141 aggcaattat ggtaaagttt tcatatctgt agcaacatta atatctatat ttggtataaa
     4201 tataggttca tcaatagtaa ctcctaaatg tggttcttct ctagcagaag aaggttctct
     4261 tcctgcattt ataggaaaaa caaataaata tggtgctcca tatgtagcaa taataatatc
     4321 tttaatttgt tgtataccac ttgtgctaac tggaagtttt gaacaacttg cagtaatgag
     4381 tgtaattgca agatttgctc agtacatacc aacttgttta tcagtaatag ttttaagaaa
     4441 aagaacagat gttaaggcct cttttaagat tccatttggt cctgttatac caatggtagc
     4501 tattttaggt agcttatggt tgttacaaca agcttgggca gaagatattt caaaacctat
     4561 aactcaaaat agagttttaa taggattagg ggcaatgttg ttaatagctc ctctatatat
     4621 atttatgaaa aaaaataagg aaatcgagca taaaaataaa tcagaagatt ctaaattata
     4681 tatttcgaga gaaaaaattc aataatattt tacaaactat ttaaaaatta tttttctaat
     4741 actatagaaa taattagaaa tggagatgtt acgtatctaa aagtagttga taacacggat
     4801 attggtactt ttatagatat gggacttaca aaagacatac tagttccttt taaagctaag
     4861 acttatccat tgtttagaga tgaaaaatat ttattctata tatacttgga taaaagcgga
     4921 agaatagctg ctactacaga catagattca tatttagaaa cagaccatac ttacaatgta
     4981 ggagatgttg taaatggagt tgtatatgga ttccaaacca acaactctgc tatgatatgt
     5041 gttgacaata aatatgctgg tgtaatcctt cataatgaat actttacaga attaaaagct
     5101 ggggatgttc ttgaaaattt acatgttata aaaatatacg atgatggtaa attaggactt
     5161 tctcctagag gaaacagaaa agatgaactt gataccttag aaaataagat tttaagctat
     5221 ttagaaggtt cagatggata tatgagattc aatgataaat ctgatcctaa agatatttct
     5281 atcttattta attcaagtaa gaaaatttta aacgtgctct tggagtactt atgaagaaag
     5341 acttaattta tcaagatgaa gatggaactt atctaaagta aattatataa cacaaaaatc
     5401 gaagtaaatt atgaaagata ttttctattt catatttact tcgatttttt atttaaatta
     5461 ctacctctat ttaaattact acttatattt attgtgcatt tactacctct attatgtcta
     5521 attcacaaat ctttttaatg ggttttctta ctgcaagcaa agttattatt atcatagtta
     5581 ctacaatgat aaacagtaaa tcaaaaggta tttgccagtc tatatggaat tttaatgtta
     5641 ctaaggatac aaatatatat ctatgtaata ttaatccaag tacactcccc aataaacagc
     5701 cacttacagc atatgtactt gattctacta ttaccatttt tcttaattgc ttattggaca
     5761 tgcctattgc acgcataata ccatagtaat ttattctact tgtaacgctt atattcatac
     5821 tgtttatgat attaaacata cttataatcg caattataaa tacaaaccca tatacaaaaa
     5881 ctgccatagt gtaaaatgaa tttttagcct ctaagttacc ttgtcgctta tctcgaacct
     5941 ctaaattatc atctagaaca tttcttatat tttggattaa atcttcagga gcatcttcat
     6001 caacttgagt atcaattatt atatagtctt ttatatctgt tatactagta aaagtatttt
     6061 cagatgttat aatatttcca atatatttgt ctttaacatc aaatgaaatt gaagaaagta
     6121 tacctgctac ctctatctct ttcttagaat ttccattttt aatatatatc ttatctccaa
     6181 tattaaaccc atgaccttct tctaccaatc cgatattttc tgactctaat ttatcttcat
     6241 tgatttcccc ttttactaga tactttttgg cccagtcaaa ttgatttttt tcataagaaa
     6301 ttagctgaga tgttgcctcc ttattattgt atgtagcctc tacatccgtc atcattctac
     6361 tgtatacttt ttttattcct ttgatattac ttatttcatt aatatcgtct ttagtaagaa
     6421 cagacttaaa gtcagcgtca acaatagata tatgagcagc ttctggtttt ataggtttaa
     6481 gtgcctgata cataaagtct ataaatacac taaaacctaa aaacaataca atactaatag
     6541 caaatgaaga agtcataagt ataaaagatt tttttcttga aaaggcatga tttattccca
     6601 tagaaacatc aattttagaa cttcctgcgt tagcaatttt acttgctttt ccactagtta
     6661 aaatttcttt gaacattaga ttaccacgta tagcacttag tggagacacc ccagaagctt
     6721 tttttgctgg aacaatagct gataatatta ctgttaaaaa tcctactatt attccaaaca
     6781 caataccaat aaagctaacc tttaaaattg gcatcgaacc aaataactct gtattcacat
     6841 atttgagaaa tatagaggat ataatggtta caatacatcc taataacaat cctagtggaa
     6901 tagcctttac acttagaatt aaactttcta aaacaacaaa ttttttgact tggagttttg
     6961 atgctcccaa gcaacgtaat agtccaaaaa actttattct ctctattaca cccatattaa
     7021 agctattata tatcatgata atacttgcta ttaacacaag tataaaaagt cctcctgctg
     7081 ttaagtaaag tggtttagaa taatcagagt caccttgacc tataatagaa agtaattttt
     7141 catttttacc gatttgttta tcacttaaat tgaaggtatt cttaatatca gaagtagctt
     7201 ttctaatatt aaccttgtct ttaaatgtaa tataataata atcatatgtt tctgattttt
     7261 tgatagcttg tgtacctaat attgaaagca ttaatccatg cttatctgag gctcttaagc
     7321 ttgataatga tgaatattca cctactatct tataattttt atttctacca tctgctaatt
     7381 ttattttgat aaaatctcct acttttagat taaagctttt aactgcatct acatctatta
     7441 aagtctcatt ctccacaaca ggataacgac cttttattat ttctagacct aattgttttg
     7501 ctaaatcttc atctaaacct tgtatcaaaa tctcctttga tttaaactct ccattatcaa
     7561 tgacttgtcc tctaccatag tgagctatat ctactctact acttatttct gatatagctt
     7621 cttcattaag atttttaagt actatgtgcc aatttccatc atcttttata aattgtactt
     7681 tttgacattg tatagccatg tctgctagtc caaaaatagc agtaactaaa catactgcaa
     7741 tgacaataca taaaattgta gttctatttt ttttcttatg tacctttgca tattgtggta
     7801 ctagttctaa atagcttctc attctctatt cacccctagg tctttaagca ttccatcaga
     7861 aacctgtagt atcctatctg ctgtagaagc tatatttgta ttatgagtta tcataataat
     7921 agtttggtta tatttttctg ctgtcacttt taaaagtgaa attacatcac tgctattttt
     7981 actgtcaaga tttcctgttg gctcatctgc caatataatt gatggctttg taatcaatgc
     8041 tcttccaatt gcaacccttt gttgctgacc accagataat tgatttggaa gatgactttt
     8101 tctatcttta agacctaaaa ttgtcaatac ttcatctaga taaatagtgt ctggtttttt
     8161 gtaatctagt agtaaaggaa atattatatt ttgttccaca gttaattcgg gtataagatt
     8221 atatgattga aagataaaac ctatattacg acgtcgaaaa atagtctgtt tttcttcttt
     8281 cattgaacat atttctttac catcaacaaa tatttttcct gaggttggag tatcaagacc
     8341 tcctaaaaga tttaataatg tactcttacc tgagccagat tctccaacta cagtaacaaa
     8401 ctctcccttt gaaactgtaa atgatacatc ttttaatgcc tctacttttg tatatccatt
     8461 tccatatgtt ttacataaat tattaaccac aagaatatcc atttgctcta tctcctttct
     8521 gatgattaat ataagaataa ttcatacacc ttactgtaag atgtatccta tcttacatat
     8581 tagtaagctt taaaaaactt agagtaaatg tacttccttg taaaaaatta ctttctacag
     8641 ttatggttcc attgtgctgt tctatgatag atttaactag tggcaaccct aatccaaggc
     8701 cttgattact ttttgaaaac ttactcctat aaaatctttt aaatatatga tgtatatctt
     8761 caatatgaat tccatttccg gtatcactca ctgaaataat agttgttata ggtgtttcaa
     8821 cccattctat cattatttta tcgttctctt ttgtattatc taatgcattt tttataaggt
     8881 tgcttaaagc ttctgatatc cattcataat cgcaaaacaa gtttgtattt tctagacctc
     8941 ttatagagat actctttcct tctttttctg ctctagtttc aaaactatat aaaattttag
     9001 taatcaactt ttttacgtta acactagctt tgttaagaat tattgtcttt gaatctatct
     9061 ttgtaatctt taacaagttt tgtatcaatg attctatacg ctctaattca ttttgactct
     9121 tctgcacaaa attctcaaca atttcactat tatgactctc ttcctgcaat atttgattat
     9181 acatcatcaa tgctgtaagt ggagttttta gttgatgtga tatatctgaa attgtatttt
     9241 taagaaattc tttattctgc ttttctttca atatatgtgt gtttagagat gttgacatag
     9301 catctattga accaaataac tttgataaac taccttcttc ataagtattt aaccgtacat
     9361 ttatatttcc attcataaat gaatcaattt tataactagc ttcttctatc ttgttctgtt
     9421 gcctaaaaaa atacacatac aatataaaaa taatcatagt aaagaacaca aataaataca
     9481 aaaacatagt ttttatatat ttaccttgaa acaatttcac acttggcata aaactatcat
     9541 ctgtaccaaa gtcatatcta gctgtattta agatttcttt tccttttaaa gtatatttat
     9601 cttcattagt agtaaatact gctggtattt ttgatttatc aacacccata ctatttaagt
     9661 atccagctat attataatca tgttctaaca gtgcactttt ataatcatta gttatattta
     9721 taactactat ttgacctact actataaaca tcaataaaac ggatataatc accaaaaaaa
     9781 atatttttat gtctttatta gaaaatacct tcatattaac accctcactt tataacattc
     9841 catttgtaac ccattcttct aactgttaat agaatttttg gattttcagg attatcttca
     9901 atcttatttc ttaaccttct tacatatact gacagagtat tatcatctac aaaactacca
     9961 tttccatccc ataacttatc caatatattt tttcttgtaa gtacaatatt tttatttttc
    10021 ataagtaagc ataataattt atattctgct gtagtaagct caagctcaat ttcattttta
    10081 aaaacacgat tttcaagtaa tttaacagta atattatttg attttagttc agttacattt
    10141 tcacttgtaa aattataacg tctaagtaaa gcttttattc ttgaaattaa ctcatttaat
    10201 ttaaaaggtt ttgttatgta atcatctcca cccatatcaa gtcccatcac tacatttact
    10261 tcttcatcag aagcagttaa aaatatgatt ggtacattag aagcttctct taccctttta
    10321 caaatatcaa aaccatcacc atcaggtaag gatacatcca aaagcagtaa atcataattt
    10381 ttttctttat aaatttgcaa tgcttcttct actgttcttg ctacatcaac actaaatcca
    10441 ttcttttgta tagaatattc taatccatat atcaaactta aatcatcttc aaccaacaat
    10501 atactactca tctcaatcac ttccattttt ttatttttta ctttatatat attatatacc
    10561 taacttatta tatttttact cttttaaaaa tacaatacct tgaaatcaat gatttaaata
    10621 ttttaaaatt atttttatat gattttatat agttaattta atactttgaa atcttaataa
    10681 taaaaaagct taaaatacac taagtattct aagctttatt caatcacatt ttatatattt
    10741 atattattta tttttttaaa atactatttt caatattgcc tctattaaac atttcattat
    10801 cattatctaa agagatatca cttatatttc taacagttgt tgaatagtta ccactaagtt
    10861 ctatctcact gaaatgcata tgcactatct tttgtattac aaatcccatt gatgcaagta
    10921 aaatagcaaa tattatatac tgactaaagt tactcatata ataatttata atggtatatt
    10981 catttccttt gactgttaaa tgttttgtag atagcatagt atatatatat tcagtacaat
    11041 ttataaatac atatatacca taaatagcta acactacaga taaacaatac attgtgattg
    11101 aaatctttga tattttttta aacttactat ctttacaaca cttaatattt gttatttatc
    11161 ataaacaagg ttattgtatt atcaataatt tttaggttac acttattatt agtcttgtgt
    11221 taaaacgaca gtagacttaa gagtagagtt ttattaagcc aatatttata tttgctatac
    11281 attgtacccc tattacacca tacatataac ttggataact tattttattt ttatttctaa
    11341 attatttaaa atcaatatct acaccattat cgataattgc aattaatttc atataaaacc
    11401 tctttaattt ataggctgtt cactataaag tctttatttc ttccataaaa ttttcatttt
    11461 gaattttatt caatgttttt agtgtagtta tcatgattat agatactaaa ataaatacta
    11521 ttaggataat agcccctata aatttccaat ttattaaatt gatactttca aaaggtttta
    11581 ttattatact tcttacaatt aaaataggta ttatagatat acaaaagctt actataatat
    11641 atatataact ttccaattta acaattttat ttatttcatg ttttgatatt cctattcctc
    11701 taaatagtgc aaattcccta cttctgctat taatactagt aactatagta ttgcaacagt
    11761 taaataaaga taatataact ataattccag ttactaatac cataattagt gttttttgat
    11821 tatcaatttt ttcatattca gctgtttcag ctttgtatgg aattaaaatt cctttatttg
    11881 ttttttctgt tattttgttt aactcacttt caacatattt atcattggct agtttactcg
    11941 tccatatgtc aaatctttca taagatgata ttcctgtaaa cttattaaat tgatttgttg
    12001 atattactcc gccaaattca gctcccactt gtgaggttac tggtaatgta
//
LOCUS       DAGSHT010000102        12025 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGSHT010000102 DAGSHT010000000
VERSION     DAGSHT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.104"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     regulatory      104..214
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            318..689
                     /locus_tag="MJB72_002379"
     CDS             318..689
                     /locus_tag="MJB72_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBY8263468.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            784..1731
                     /gene="trxB"
                     /locus_tag="MJB72_002380"
     CDS             784..1731
                     /gene="trxB"
                     /locus_tag="MJB72_002380"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBY8263469.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            1835..2152
                     /locus_tag="MJB72_002381"
     CDS             1835..2152
                     /locus_tag="MJB72_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBY8263470.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            2421..3707
                     /locus_tag="MJB72_002382"
     CDS             2421..3707
                     /locus_tag="MJB72_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBY8263471.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            3819..4292
                     /locus_tag="MJB72_002383"
     CDS             3819..4292
                     /locus_tag="MJB72_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3951..3953,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBY8263472.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            4324..5634
                     /gene="grdB"
                     /locus_tag="MJB72_002384"
     CDS             4324..5634
                     /gene="grdB"
                     /locus_tag="MJB72_002384"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5371..5373,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBY8263473.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            5890..7422
                     /locus_tag="MJB72_002385"
     CDS             5890..7422
                     /locus_tag="MJB72_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBY8263474.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            7433..8578
                     /locus_tag="MJB72_002386"
     CDS             7433..8578
                     /locus_tag="MJB72_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBY8263475.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            8888..9967
                     /locus_tag="MJB72_002387"
     CDS             8888..9967
                     /locus_tag="MJB72_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY8263476.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            10080..10679
                     /locus_tag="MJB72_002388"
     CDS             10080..10679
                     /locus_tag="MJB72_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263477.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(10865..11737)
                     /locus_tag="MJB72_002389"
     CDS             complement(10865..11737)
                     /locus_tag="MJB72_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8263478.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT         4691 a         1349 c         2305 g         3680 t
ORIGIN      
        1 aatgtttata tacttaagtc aacagagata gaaaaaagct atttatagaa aacaaaggat
       61 aaattgctta attttaaatt ttctgaaaaa aataataatc cggatgaaga gtatgggaga
      121 ctgctttata aaaagcgacc gaagggacaa gtttaatctt acttacccaa aagattagat
      181 gaaattctca ggtaaaggtg actgtacttg gacggggctc tgaaaatgat tttaacaggg
      241 aagacaaatt gggagatttg tttttcctgt tttttattta tcaagtaata aataaaagtt
      301 tgaaagagag taaaaaaatg ataataatta cgaataatcc aaaagtaaaa gaagaagttc
      361 aaggcagaga agttttgttt aaagacacaa cttatattgg aatcttagaa gcaagtagag
      421 atttgataca tgaaggttat gaacttttat ctcatccact ctatgggagt gtaaagccaa
      481 atgaaacacc ttacaggact gttatactaa agaaaggaaa tcgtttggat ataaactctt
      541 taaccttaat tgaagaagca atcatcactg caagtaaatt tcagaataat aaaaagactc
      601 caaaatggac agaaagtgtt caagatgatt ttagagtaat tgattatgat atattttata
      661 atacaataca aagaatgcag tatgaataaa aaaattgtta aaaagttatt aaaacagcct
      721 aaatattaaa tctaatttaa tattaaataa aatgagaaat ttaattgggg aggataagtc
      781 aaaatggaaa atgtgtatga cttagtaata attggttcag gaccagcagg gcttgcagca
      841 ggtctttatg gagcaagagc aaagttaaaa actctaatcc tagaaaaaga caagactggt
      901 ggacaaattg taataacaca tgaaattgca aattatccag ggtcagttcc aaatgcaaca
      961 ggaccaagtt taatagctag aatggttgaa cagtgtaaag agtttggagc agaaatgcta
     1021 agagacaaca ttgttgatac tgagctagat ggagatataa aagttttaaa aggggaaaag
     1081 gcagaatata gagcaaaagc agtaataata ggaacaggag caacaccaag aaagattggt
     1141 tgtcctggag aaaaagaact tactggtaaa ggggtttcat attgtgcaac ttgtgatgca
     1201 gatttctttg aagattttga agtatttgtt gtaggtggag gagacagtgc attagaagaa
     1261 gcaatgtatt taactaaatt tgctagaaaa gtaacaattg tacatagaag acaaggattt
     1321 agatgtgcta agagtgttga agaaaaagca aaagctaatc caaagataga gtttttatta
     1381 gatacagtaa ttgaagaaat caaaggtgat ggaatattag aatcagttgt atttaaaaat
     1441 aaagttactg gagaaactca tgaatacttt gcagatgaag aagatggaac aatgggagta
     1501 tttgtgttcg ttggattaga tgctcaaact gatttattca aaggtaaagt tgatatggat
     1561 gaaaaaggtt acataataac tgatgaagat atgagaacta acataccagg cgtatttgca
     1621 gcaggagatt gtagatctaa gactctaaga caggttgtaa ctgcaactaa tgatggagca
     1681 attgcatcta tagtagctga gaaatacatt gatgaaaaat ttgggaacta gaatctgtaa
     1741 attaagaaat agtgtctgta aatttaatga ataatattta aaattaaaat taatatttaa
     1801 aataaaaata aaaatttcaa gggggaataa gaaaatgtta gatttagata aggctacatt
     1861 tgaagaagaa gttttaaatg cagaaggttt tgtatttgtt gatttttgga gtgaaggttg
     1921 tgagccttgt aaagctttaa tgccagatgt tcataaatta gctgagactt atggggataa
     1981 aataaaattc tgtaagatgg atacaacaaa agctagaaga ttagctataa aacaaaaagt
     2041 attaggtctt ccaactatgg ctatatacaa agatggagaa aaagtagacg aagttactaa
     2101 ggatgatgct acagttccta atatagaaaa catgattaaa aaatatcttt aatatgttga
     2161 taaattatta acattaaaag aaaaaatcta tttttatctg aagataagaa gaaaaaggtt
     2221 tttatgtcca actttgttaa aaaaataatt ttaaataaaa taattttttt acaataaata
     2281 ttttttagta ataaaataaa gatttaaggc ataaaaacac aataatatat ctttaaaaaa
     2341 ataagataaa ttgtaaaatg atataatttt gagaaaaact caaatgcata tacacaaatt
     2401 aaataacagg aggtgcaagt atgcgtcttg aattagggaa aatctttata aaagatattc
     2461 aatttggtga tgtaacagaa gtaaaagatg gagtattatt catcaataag caagagatgc
     2521 tacaagaaat tggtggagat gagcatatta aatctataga tatagaatta gctcgtccag
     2581 gtgaaagcgt aagaataaca cctgtaaaag atgttattga gccaagagta aaagttgaag
     2641 gtaatggggg aatcttccct ggtatcatgt ctaaagttga cactgtagga gaaggaaaaa
     2701 ctcatgcttt aaaaggtgta gctgttgtaa ctactggaaa aatagtaggt ttccaagaag
     2761 gtataataga catgactgga gaaggagcta agtacactcc attttctaaa ttaaataatg
     2821 tagtagtaat agctgaacct attgatggtt taaaacaata cgctcatgaa aaagctgtta
     2881 gaatgatagg attcaaagca gcaatgtact taggagaagt agcaagaaat cttactccag
     2941 atgaagtttc agtatatgaa actaaacctc ttcttgaatc aataaaagaa tatccagagc
     3001 ttccaaaagt aggatatgtt tacatgcttc agactcaagg tttattacat gatacttatg
     3061 tatatggagt agatgcaaaa caaatagtac caactttatt atatccaaca gaattaatgg
     3121 acggagcaat tgtaagtggt aactgtgttt ctgcatgtga caaaaaccca agttatgttc
     3181 atataaacaa tggggttgtt gaagacttat atgcaagaca tggtaaagat ataaactttg
     3241 taggtgtaat tataactaat gaaaatgttt atctagcaga taaagaaaga tcttcaaact
     3301 ggacttctaa attatgtaaa tatttaggat tagatgcagt tatagtttca caagaaggtt
     3361 ttggaaatcc tgatactgac cttataatga actgtaagaa gatagaaatg caaggtgtta
     3421 aaacagttat agttacagat gaatatgctg gacgtgatgg aggttctcaa tcacttgcag
     3481 atgcagatgt aagagctgat gctgttgtaa caggtggaaa tgccaaccaa gttgtagttc
     3541 ttcctaagtt agataaagtt attggacatt tagaagtagt agatgtaata gctggtggtt
     3601 ctgatggaag tttaagagct gatggaacta ttgaagttga aatccaagct ataacaggtg
     3661 caactaatga aacaggattt ggtcatttaa ctgctaaagg atattaagaa gtattttatt
     3721 aaacgattga gtaatgttaa taaaacatat tatagataat tataataagt gttatagata
     3781 tataaaaata tattagaaat ataataagga ggattattat gagtttactt agtaataaaa
     3841 aggttcttat aataggtgac cgtgatggta taccaggacc tgcgatagaa gaatgtgtaa
     3901 aaacagtaga aggagcagag gttgttttct catctacaga atgctttgtc tgaacagctg
     3961 ctggggctat ggacttagaa aatcaaaaca gagttaaaga tgctgctgat aaattcggag
     4021 ctgaaaatgt tgtgatttta ctaggtgctg ctgaagccga agctgcaggt cttgcagccg
     4081 aaacagtaac tgctggagat ccaactttcg ctggaccact tgctggagtt gccttaggat
     4141 taagtgttta ccacgttgtt gaggaaccaa taaaatcatt atttgatgaa agtgtatatg
     4201 aggaccaaat aagtatgatg gaaatggttt tagaagttga agaaatagaa gaagaaatgt
     4261 ctggtataag agaagaattt tgtaaatttt aatgcctaaa agcaaaaggg gtgaaactaa
     4321 gagatgggaa aactaaaagc agtacattat attaatcagt tctttgctgg tataggtgga
     4381 gaagaaaaag ctgatactaa gccacatgtg gcagaaactt tacctccaat aagtttacaa
     4441 cttgataaat tattaggtga agatattgaa atagttggaa cagtagtttg tggagatagc
     4501 tatttcaatg aaaatataga tagtgcaagt gaagaagttt tatcaatggt aaaaggtttt
     4561 gaaccacaac ttttcatagc tggtcctgca tttaatgctg gtagatatgg ggttgctgct
     4621 ggaacaataa ctaaagttgt aaaagatgct ttgaatatac cagctttaac aggtatgtat
     4681 gttgaaaatc caggagcaga tatgttcaaa aaggatgttt atgtagtaga aacttctgat
     4741 tctgcagcag gtatgagaaa ggcacttcct aagatagcta aacttgcagt aaaacttgct
     4801 aatggagaag aaattggaac tcctaaagat gaaggataca tagctagagg aataagagtt
     4861 aactacttcc atgaagacag aggttctaag agagctgttg acatgttagt taagaaaata
     4921 aaaggtgaac catttgaaac tgaataccca atgccaaact ttgatagagt tgacccaagt
     4981 aaagctgtaa aagatttatc aaaatgtaaa atagctttag ttacttctgg tggtatagtt
     5041 ccaaaaggaa atccagatag aatagagtct tcttcagctt ctaaatatgg aacttattct
     5101 atagcaggag ttatggattt aacagaagag acttatgaaa ctgcacatgg tggatatgac
     5161 ccagtgtatg caaatttaga tgcagataga gtattacctg ttgatgttct tagagattta
     5221 gaaaaagaag gtgtaattgg aaaattacat gagactttct atacaactgt aggtaatggt
     5281 acatctgttg caaactctaa aaaatatgct tctgaaattg gagcagcatt agtggctgat
     5341 ggtgtagatg cagtaatatt aacttctaca tgaggtactt gtactcgttg cggtgcaacg
     5401 atggtaaaag aaatagaaaa aactggactt ccagtagttc atatgtgtac agtagtacct
     5461 atttcattaa cagtaggggc taatagaata gttccaacta tagcaatacc tcatccactt
     5521 ggaaatccag cgcttgaccc tacagaagaa aaagccctaa gaagaggcct tgtagaaaaa
     5581 gcattaaatg ctttaactac agaagtagat ggtcaaacag tatttgaaaa ataaaaataa
     5641 cacacacata tattatatag ataaaaatat aaacacacac taaaaataaa aaataactat
     5701 acacaaaaaa ataatataga tttcatgaat ggataaattg gagttattca ttcatgaaat
     5761 cttaaaaaat gccaaaatac ataaacaata atagaatcaa atatacaata aaatacaatg
     5821 attagagctt ttaataagtt taataagatt aaaaaataag tgttcataaa atttatggag
     5881 gtgcacaaaa tgacttatcc agttttaaaa ggggcaggct atgtacttat tcatactcca
     5941 gatatgatag tgcaaaatgg aagtacttgt acagttgaaa gagcaacaaa tccagattca
     6001 gaatttttaa aagaagtaag taatcatata agaagttatg aagatgtagt taactacatg
     6061 ccaaatcaag tttatatagg aaatagaaga ccagaagaat taagagattt accaatgcca
     6121 tggtgtgaac aaaagattga gggaactaga aatggtaagt ttggagaaat aatgcctcaa
     6181 gatgagttca tagctttaat gcaaataagt gatgcatttg atttagtaaa attatcacaa
     6241 gaatttattg atgaagtaaa gccaaaaatc gaaaataact accctgaaat tgcaccattt
     6301 gtaggtaaat taaagggtga tgatatagaa gaaggtaaag aattagtagc aacacacata
     6361 gctgaaggat tataccatga tggaaaattt gttggttatg taaaaagagc acatgatgta
     6421 gatgttaact taaatgccca tacaatgttt gaaaatctag tagtaaaagc atctggagtt
     6481 ttatctgcta ttcaaatgct aagacatagc aaaatagacc ctgctgaaat agattatgtt
     6541 attgagtgtt cagaagaagc ttgtggagat ataaaccaaa gaggtggagg aaactttgct
     6601 aaatcaatag ctgagatagc aggacttcaa aatgcaactg gttctgacac aagaggattt
     6661 tgtgcagcac caactcatgc attaattcaa gcagcagctt tagtaaaagc tggtatacat
     6721 aaaaatgtaa tggtagtagc aggtggagca agtgctaaac ttggaatgaa tgctaaagac
     6781 catgttaaaa aaggtcttcc tgtacttgaa gatgttgttg gaggatttgc agtacttgta
     6841 tctgaaaatg atggtgtaaa tccagttata agaactgatt taacaggtaa gcatactgtt
     6901 ggaactggtt cttcaccaca agcagttatg acagcactta taacttcagg tcttgataga
     6961 gctaatttaa aaataacaga tgttgatgtg tattcagttg aaatgcaaaa cccagatata
     7021 actaagccag caggagcagg agatgttcca gaagcaaact acaaaatgat aggggcactt
     7081 gctgttaaac gtggagactt agaaaagaaa gaattgaaag attttgtttc taacaaagga
     7141 ttaccaggat gggcaccaac tcaaggacat ataccatcag gagctcctta tataggattt
     7201 ttaatagatg atttaactac aggaaataga aatagagcta tgatagttgg aaaaggtagt
     7261 ttattcttag gaagaatgac taacttattt gatggagtat cttttatagc tgaaagaaat
     7321 acaggtgtta ctgaagagac ttcaggaata tctaaggatg aaattaagaa gataatagct
     7381 gaatctatga aaaaactagc attagatatg ttagaagaat aggggtgagg atatgtcaaa
     7441 aaaagtaatc gcagatgtat tcttagaagt agctaatgca atagaaagcg gcgaatttgg
     7501 caaaaaagta aaaattggtg ttactacact tggaagtgaa catggtgtag aaaacatggt
     7561 taatggtgca caattagcaa aatctaattt atttgatatt gtgcttattg gaccaaaagt
     7621 ggaaactgac cttgaagtag ttgaagttaa tgatgaaaaa gaaatgcatg caaaaatgga
     7681 agaattatta gattcaggat atattgatgc ttgtgttact atgcattata attttccaat
     7741 aggtgtatct acagtaggta gagtaataac tccagcaaaa ggaaaagaaa tgatacttgc
     7801 aacaacaaca ggaacaagtg ctactaatag aatagaggca atggtaagaa atgccattta
     7861 tggtattgct acagctaaat caatgggtaa caaatgccca aaagttggaa tacttaatgt
     7921 tgatggagca agacaagttg agaagtgctt aaaagaatta aaagataatg gatatgacat
     7981 ggagtttgcg gattcaatta gagcagatgg tggatgtgta atgagaggta atgatttact
     8041 tgtaggtgca cctgatgtta tggttactga tacattatct ggaaatatat ttatgaaagt
     8101 attttcttca tatactacag gtggagatta tgaagctcaa ggctttggat acggacctgg
     8161 agttggagag gactatgata gaaaggtact tatagtatca agagcatcag gttctccggt
     8221 agtagcaaat gctttaaaat acgcttatga tgttgtaaag ggagatataa gtaatgttgc
     8281 aagaaatgaa tttgcaaagg ttaaaaaagc aaaatttgat gacattatat cctctcttac
     8341 taaaaaagaa gttaaagctg aaaaagtaga agttaaaatg ccagataaag aaattgttac
     8401 tcgtcaaata gctggtgttg atataatgga tttagaagat gcagtatctg agttatggaa
     8461 aaacggaatc tatgctgaaa gtggtatggg ttgtacaggt ccaatagttt tagtaaatga
     8521 agctaaagga gacttagcag ttgaaacttt agttaaagct ggatatactg caaaataagt
     8581 acatttttta tatgtaaata agaacatata gttataattt tattgattat aattatatgt
     8641 tctttttatt ttatatcttg tcaatattta tatattaaca caatccattt atttgattct
     8701 ctagctaatt tattatattt attcagttaa aattaagctt tctaactaaa taaaaactaa
     8761 tgaagtatta aaatgaaatt gataaaatat gaaaaaattt taggaataaa gtatctgtta
     8821 gataaaaata agattaatta gtgatataat ataattaaga taaaatttat tatttggagg
     8881 caaaatcatg agcagagtaa aaaatgtagt agaattgttg gaaactaaag gtgttgatgc
     8941 actatatcta acaaagaaaa ctaatgtaaa ctatataagt ggttttcctg atgaagaagc
     9001 atatgcagtg atttgcaaag atggaaactt tttagttact gatagtagat atatggagct
     9061 tgcagaaaag gtttgcaaag attttgaaat tataaattgg cataattttg atagaagtgt
     9121 ggcaaaggca gttaaaagtg tatgtgataa agtgggtata aagaaacttg ggtttgaaag
     9181 aactaatata gtgtttgata aatatgaaga acttaaaaat ctgattgaga aggataatgg
     9241 agaattaata cctacagaaa atattgtaga gactttgaga tatgtaaaag acaaagatga
     9301 aatcaaaaat acaagaaaag cttgtgaaat agcggataag gcattagaag aattgattcc
     9361 acatataaaa gctggagtta gcgaaattga actggctaca aagttagagt actttatgaa
     9421 aatgaacgga gcacaaaata tagggtttga aactatactg atatcaggag caaagacttc
     9481 attgcttcat ggtaagccta gtgataagat tatagaaaaa ggtgattttg tacttataga
     9541 ctatggcgct atgtataatg gctatatatc tgatacaact agaactttta tagttggagg
     9601 agcttctgaa aagcaattag aaatatataa tttagttaag gaagctcaaa atgtaggggt
     9661 tgagaacatg aaagctggtg tacatgcgac aattccagat gctgagataa gaaaagttgt
     9721 caaaaaatat gaagactatt actatcaagg tataggacat ggagtcggta gagatgttca
     9781 tgaagaacct ttcattggta attatggtga taaaatcata gaagagggat gtataataac
     9841 aatggaacca ggtatttatt tccctggatg gggtggagtt agaatagaag atactgtttt
     9901 gattactaaa aatggtccag aaagacttac taaattccct aaagatttga tgattttaga
     9961 caaataattt aatttatata tattttttta cataaaatat aacataatct cactattaag
    10021 ctctatttac atattatagt aaagagggct taattttatt gtgagaggtg atagtatata
    10081 tgtataaaaa acataaattt ataaaaaata taaaaaaagc ctggtataaa atcaaattaa
    10141 ttgatagatg ccttataatt gtaatggcaa tattaatgtt tcaatctata tataatttat
    10201 ttgttaatga agtaaattca caagatacta caactattga tgtagtagta agaaccactt
    10261 cagcagctat atttgggtat tttttaagtg ctaattttat aaagagacct ttaaggaagt
    10321 atacaaatac tgatgtatct tcaaatcttt ttatagataa taatttatcc ccaaaaggtc
    10381 aagagtctaa tcaaaataat ataatgaatg tcaaaaatac cattggattt acttcagaag
    10441 ctgataatta tgaaaagaaa atacctatta ataataatga aggttttgaa gaaggagaaa
    10501 ctagtgaatt gcaaattatt atagctactg taatttgtat agtagcatta acagtacttt
    10561 ttgttgtaag aaattttaca actacaacat cagcttcttt aggtactatt tcacagatga
    10621 gagattttgt ctcaggatgc gtgggttttt tgctaggttg tccaagtaaa cctgattaaa
    10681 aaagtattat caataaaagg tattttaaaa agtatcagta taataatatt tagatttaaa
    10741 tgtatacaat tataattcta aacattctat attgtatttt tatactgata ctctaaacta
    10801 tactatttat taaaatattt taacattgta tttaaacaaa atattatatc tgtataatat
    10861 aaatttagtt tatatgaata gatgccatat actcaataaa aagtttcata ctatgagata
    10921 tgtattgatt tcttctccaa agaaccccaa tattcgcagt aattgcacgt tctaatggta
    10981 ttgaacagag attttctgta ttttgaaata tttctttata caatattgtt gaagccaagt
    11041 cattttctag taaataacgt atggtagaaa gttgatttga gtgtaaaatt atatttggct
    11101 tagctttttc aagtcttttt gtaataatct tatagtgata agaaccttca gataagatta
    11161 caaaaggtac attttttaac atataattcg atataatggg atgttttgcc aattcatttt
    11221 tgggatttgt aactaagtat aattctgttt tatagattgt ctttgaatct atatcatcat
    11281 aaatatcact gtcaattata ccaatacaaa aatcaagtgt agattcttca atcattttag
    11341 ctccaacaaa tgttggaact tcaaaaattt taagctctat atctggataa attacattaa
    11401 atctaggaag tatttttgaa agcaaaaacg tacctaaaac tgtaggaata cctattttga
    11461 gagaagcttt tctctttttt cctaaatcaa gtgcgtcaca ttgaaaatcc tcaaattgtt
    11521 ttataaaatg ttttgacttg tctaaaaaaa tttttccttc aggcgttaac tctattttat
    11581 tacctattct gtaaaaaagt tcaaagccaa gttctttttc taattcttta attgctaatg
    11641 ttatggatgg ttgagaaata tgaatacttt ccgcagcctt agtcatgttt ttatatttac
    11701 agacagtttg aaaatagtat aaatagttta tattcatttg gtattagttc ctttcattta
    11761 catattagtg acaagtatat aattaacaat agcataagtt ttgtatattg ttgtaaaaaa
    11821 atagaatagt tttaaactat catagcataa gtttttgata ttttcaatct attaagaata
    11881 gtgctattat taaattaaca aaagagaata gctggtatgg tttagagtaa atcaatctat
    11941 attacagtta tatcaacgaa tatcaaaaaa ttatctatta gaaaataaaa attattttta
    12001 caattaaatt gttaattaaa tcata
//
LOCUS       DAGSHT010000103        11819 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGSHT010000103 DAGSHT010000000
VERSION     DAGSHT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.105"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            95..970
                     /locus_tag="MJB72_002390"
     CDS             95..970
                     /locus_tag="MJB72_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBY8263479.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            988..2478
                     /gene="cls"
                     /locus_tag="MJB72_002391"
     CDS             988..2478
                     /gene="cls"
                     /locus_tag="MJB72_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBY8263480.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2671..2955
                     /locus_tag="MJB72_002392"
     CDS             2671..2955
                     /locus_tag="MJB72_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBY8263481.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            2988..4616
                     /gene="groL"
                     /locus_tag="MJB72_002393"
     CDS             2988..4616
                     /gene="groL"
                     /locus_tag="MJB72_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBY8263482.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4878..5618
                     /gene="larB"
                     /locus_tag="MJB72_002394"
     CDS             4878..5618
                     /gene="larB"
                     /locus_tag="MJB72_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBY8263483.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5702..6436
                     /gene="larC"
                     /locus_tag="MJB72_002395"
     CDS             5702..6436
                     /gene="larC"
                     /locus_tag="MJB72_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBY8263484.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6466..6885
                     /locus_tag="MJB72_002396"
     CDS             6466..6885
                     /locus_tag="MJB72_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBY8263485.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      6945..7046
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7267..8802
                     /gene="guaA"
                     /locus_tag="MJB72_002397"
     CDS             7267..8802
                     /gene="guaA"
                     /locus_tag="MJB72_002397"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBY8263486.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGIPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9341..10771
                     /locus_tag="MJB72_002398"
     CDS             9341..10771
                     /locus_tag="MJB72_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8263487.1"
                     /translation="MILNQIFIEAGGIMRNKIIMGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10919..11269)
                     /locus_tag="MJB72_002399"
                     /pseudo
     CDS             complement(10919..11269)
                     /locus_tag="MJB72_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11264..11785
                     /locus_tag="MJB72_002400"
     CDS             11264..11785
                     /locus_tag="MJB72_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY8263488.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
BASE COUNT         4700 a         1184 c         2253 g         3682 t
ORIGIN      
        1 tctttgtgtt aaaataagta aaatgcacat attagagcta agtttttaag taaataaaca
       61 aagtgaaaat ataaatatta aggagatttg gtatatgaat gtttatgaaa aaggtaatgg
      121 agttatacta gaaggagtaa cagattttga cccagtacat atatttgagt gtggtcaatg
      181 ttttagatgg cacaaacaag aagatggctc atacactgga gttgcaaagg ggagaatatt
      241 aaatgtcaaa aaagagaatg ataaaatata tttaaataat actaatttaa aggagtttaa
      301 cagcatttgg tataattact ttgatttagg aactgattat acagaaataa agaataagct
      361 aaaaaatatg gatgaatatt taaataaggc tactgaattt ggatggggaa taagaatatt
      421 aagacaagat ggatgggaaa tgcttatatc atttataatt tcctctaata ataggattcc
      481 tatgatacaa agagctatag aaaatttatc aagaaagttt ggtaagtata taggtgaata
      541 tgaaggcaat gaatactatg cattcccaac accagaagaa ctaaataagg cctcacaaga
      601 ggaaataaga gcatgtcaaa caggttttag agataagtac ataaaaagta ctacacaggc
      661 agttatagag aataatgatg aggtatctga atatacaaat ttaagtacag aggattgtag
      721 aaaagagctt ttaaaattta atggagtagg accaaaagta tgtgattgta ttgcattatt
      781 tggtatgcaa aaatatgatt cttttccagt tgatgtttgg gttaagagag ttatgcaaga
      841 attttatata gatgaagaca tgagtttacc taaaatgaga acctatggaa tcgataaatt
      901 taaagaaatg tctggatttg ctcaacagta cttattttac tatgcaagag aactcggtat
      961 aggaaaataa attaaggggg agaaattatg ggagtaatag gaacaatatt tctgttctat
     1021 ttaattatat catatctagc tggagccata atttctgtga ttatattact tgaaaataga
     1081 gaccctgcaa aaacaatgtc ttggctattg atgtttatta tatttccagg tgtaggttta
     1141 atgatatatg ctatttcagg tagaaatatt agaaaaagaa aattatttaa gactcaaaaa
     1201 ctagctaata atataaaaga aaagaaacta tttgatacat tagaaaagat aacagaaata
     1261 gttgaattag aaaaagaatc tataaaacaa aataagcttt taagagatga agaggatggt
     1321 agttatagaa aaagggtaat aaatatgttg ttaaagactg gtatgtttcc ctttactaaa
     1381 aataataaag tggatgtatt tgtagatgga aatgaaaagt ttaaaagact tatagaagat
     1441 ataagagaag ctaaagacca tattcattta gagtacttta tcatcaaaga tagtgaaata
     1501 ggtagggtgt taaaagaaga acttataaaa aaggctaaag aaggcattaa gataagaatt
     1561 ttatatgatg atgtaggatg ctggagattt tggtttaata gaaaattttt ccgagaaatg
     1621 agggaagtag gtatagaaat tgcagcattt ttacctacaa aatttcctat aataggaggg
     1681 aaattaaact atagaaatca cagaaaaatt gttgttattg atggaataat tggatataca
     1741 ggtggaataa atataggaga tgaatattta ggcaaaaatg ataagtttgg atattggaga
     1801 gatactcata ttaggattaa gggtatttct gtatacatgc ttcaaatgac atttctgatt
     1861 gattggtatt atacgacaaa agaagtatta gtgactaaaa attatttccc aagtgtaggg
     1921 aatgttggtg aaagtatgat acaagttgtt gcgagtggtc cagacagtga ctgggaagat
     1981 attcattatg cttatttttc tgcaatatgt caggctagaa aaaatgttta tatagaaact
     2041 ccatatttta ttccagatga aagtctacta aaagctataa agagtgctgc tcttagtgga
     2101 gttgatgtga gaataatatt tcctaaaatt gcagaccaca agatagtaaa tatagcatct
     2161 tattcatatt ttgaagaaat attaagagca ggtggtaaag tttatttata taataaagga
     2221 tttatacatt ctaaagtcgt aataatagat gataaaattg cttctgcagg tacagctaat
     2281 atggatttaa gaagttttat gcttaacttt gaggtaaatg catttatcta tgatgaagaa
     2341 gttattaggg taatgacaga tgatttcttt gaggatttaa gttactgtga agaacttaat
     2401 ttggaggtat ttaagaatag aaatatcata caaaaaatca aagaatctgt agcaagatta
     2461 ttttcaccaa tattataaaa aattattaaa aaaggtattg aaaattatgt gtgattggta
     2521 tattataata attgttagca ctctattaga tagagtgata ataaataaaa agtatatata
     2581 taaatctaca tatataagta gtattaattt aaaaataact aaaaacatag gcgaactagt
     2641 ctatacttaa tattttagga ggcgtgaata atgaaaataa gaccattagc tgacagagta
     2701 gtaattaaaa aagtagaagc agaagaaaaa actgcaagtg gaatagtttt accaggagca
     2761 gctaaagagc aacctcaaat agctgaagtt gtagaagttg gaccaggtgg aatagttgaa
     2821 gggaaagaaa taaaaatgga attgacagtg ggagataagg ttatattcca aaaatattct
     2881 ggaacagaag ttaagataga aggacaagaa tacacaatac taagacagag tgatgtatta
     2941 gctgttattg aataaatata gaataaattt attaggaggg gtttaaaatg gctaaagaaa
     3001 ttaaattttc agaggaaaca agaagagctt tagaagctgg tgtaaataaa ttagcagata
     3061 cagtaaaagt aacattagga cctaaaggaa gaaatgttat attagataaa aaatttggtt
     3121 ctccacttat aactaatgat ggagtaacta tagcaaaaga gatagaacta gaagatagat
     3181 ttgaaaatat gggtgctcaa ttagttaaag aagtagcaac taaaactaat gatgtagctg
     3241 gagatggtac tacaactgct acagttttag cacaagctat aataagagaa ggtttaaaga
     3301 atgtaacagc aggggctaac ccaatacttt taagaaaagg aatacaaaaa gcagtaacag
     3361 tagcagtaga agaattaaaa aatcaatcaa gaatagtaga aacacaagag gctatatctc
     3421 aagttgcttc tatatctgct ggtgatgaag aagttggaaa attaatagca gaagctatgg
     3481 aaatagtagg taaagatgga gttataactg ttgaagagtc tcaaactatg aatactgaat
     3541 tagatgctgt tgaaggtatg cagtttgata gaggatttgt ttctgcatat atggttacag
     3601 atgtagataa aatggaagca gttttaaatg acccatatat attaattact gataaaaaaa
     3661 tatctaacat acaagaatta ttaccagttc ttgaacaaat agttcaacaa ggtaaaaagt
     3721 tattgataat agctgaagat gtagaaggtg aagcattatc tacattagta gtaaataaat
     3781 taagaggaac atttgatgta gtagcagtta aagctccagg ctttggagat agaagaaaag
     3841 aaatgcttca agatatagca atacttacag gtgctcaagt aatatcagaa gaattaggtt
     3901 atgatttaaa agaagctgat ttatctatgc taggtagagc ttcatctgtt aaggtaacta
     3961 aagaaagtac tacaatagta gatggttctg gagataaaaa agctatagaa gatagagtaa
     4021 ctcaaattaa acatcaagta gagcaaacta cttcagattt tgatagagag aaattaatgg
     4081 aaagattagc taaacttgct ggaggagtag ctgttgtaaa agttggagct gctacagaag
     4141 tagagttaaa agaaagaaag ttaagaatag aagatgctct taatgcaact agagcagctg
     4201 tagaagaagg aatagtagct ggtggaggaa ctgcttttgt tagtgttata ccagcaatag
     4261 gaacactaat tgagagttta gaaggagaag ttaaactagg tgctcaaata gttaaaaaag
     4321 cgttagaaga gccattaaga caaatagcta taaatgctgg tcttgaaggt gctgtaattg
     4381 tgcaaaatgt tgttaattct gaagcagaaa ctggatttga cgcattgaat gaaaaatatg
     4441 taaatatgat agaagctggt atagttgacc caactaaagt tagtagaagt gctttacaaa
     4501 atgctgcatc aatagcaagt actttcttaa ctactgaagc tgctgttgct gaccttcctg
     4561 aaaaagaaga tgcaggaatg ccaggaatgg gtggcggaat gcctggtatg atgtaattta
     4621 aaaaatttaa aatataaatt ttaatataaa gaactatttc gacttagatt ttaaactcta
     4681 agttgaaata gttcttttgt atatatctaa tttttgtata tctataaatt caatacacat
     4741 atttagataa tcagtatcta gtttgtaata atattttaga gtagtattgt tattttttag
     4801 gttatgattt tagatatata ttaattaagg tataattgtt gatagagaat aaatcaaatt
     4861 taaaaggatg cgataatatg gacttaagaa aattgcttga agaagttaaa aatgaaaata
     4921 ttgatataga ttttgcgtta gaaaagttaa aagatttacc ttatgaagac cttggatatg
     4981 caaatataga tcatcataga gaactaagaa atgggtatcc ggaagttatt tattgtgaag
     5041 gtaagagtga tgagcatatt ttagggatta ttgataaaat gaatcaaaaa agttctaaca
     5101 tattgggaac aaggtgcaga aaagaaactt ttctgaaaat taaagaaatc tataatcatg
     5161 ctgagtacga agaggcgtct aaaattctta agattcaaaa tcatgatata gagaatatag
     5221 gaaaaggtaa aattgtgatt gctacagggg gaacttctga tataccagta gcagacgaag
     5281 cttatcatac agctaagttt ttaggtaatg atgttgatag aatatatgat gttggagtgg
     5341 caggtataca taggctttta aataaaagac ataaaataga tagtgctaga gtaatagtag
     5401 ccgttgcagg tatggaaggt gctcttgcaa gtgtaattgg tggactggtt gatgttccag
     5461 taatagctgt tccaacttct gttggatatg gagcaaattt tggaggatta gcagcattac
     5521 tagctatgtt aaatagctgt gcatctggaa tatctgttgt aaatattgat aatggatttg
     5581 gagcaggata tttagcatca atgataaata aactgtaaat acattgatat aaattaaaca
     5641 aaactatata ataaaattta atttctatta tatttttact attaaaattg aggagacata
     5701 tatgagtgaa aaaatacttt attttgatat aataagcggt atatcagggg atatgaccct
     5761 tgcaagttta ttaaatttag gtgtgcctaa ggaaattttt ttggaggaaa ttcataaact
     5821 aagaatgagc gatgaattta atataaacat cagctcgaaa actgaaaatg gaatagtagg
     5881 tacaaaagta gaagtaatta caaaagaaaa acatacacat agaaatttag tggatatatt
     5941 tgaggttata gataaaagca ctctaaacga aaatgtaaaa tctaaagcaa aaaaaatatt
     6001 tatggtaatt ggagaagcag aagcaaaagt tcatggaact acaatagaca agatacattt
     6061 tcatgaggta ggggctatag attcaattgt agatatagta ggagcttcta tattagttga
     6121 cttactttgt gtagataaag tttgtgctag tacagtgtct gttggttcag gatttgtaaa
     6181 atgtgaacat ggaattatac ctatacctgc acctgcaact atagaaattt taaaaggtgt
     6241 gccaattaaa ttaaataatg taaatggaga atgtacaact ccaacaggag ctgcaatcat
     6301 aaaagttttg tgtgatgagt ttgtagatga gcttgaattt tctccaaagc aaattggata
     6361 tggtataggt cataagaagt ttgaagtacc aaatatactc agaactgtat taggtgaaaa
     6421 aaaaaagaag aattaattta tgaaattagt gcaaacattg atgatatgtc ttcagaaatc
     6481 tattcttatt tgtatgaaaa aatattagat gagaaagcat tagatattta tacagagagc
     6541 atttatatga agaagaatag accagcaaca aaagtatcaa ttttatgtaa tgaatcagat
     6601 ttagaaaaat ttattaaaat attactttta gaaactagta cttttggagt gagatataat
     6661 gcatataata gagaaattct agagagaaaa tttgtaaaat tagatacaaa atatggagtg
     6721 gttacagtaa aactagggta ttataaagga aaactagtca aggctactcc agaatataat
     6781 gaatgtaaaa ctatagcaaa aaatgaagat attcctataa aaaaagtgtt tgctgatata
     6841 aattttagaa taaaagaaga atttagtgaa aacttattga cataagtaat aaattttgat
     6901 aaattaatat tcagccttat taaataaaag taaacagaaa aaaatatata aaatataaac
     6961 tcactcgtat atgctcagaa atatggtctg agagtctcta ccaagatacc gtaaatatct
     7021 tgactatgag tgaaattatt ataccaaaag acctagttaa gtaaatgtgt aggctgagtt
     7081 aaactcccta tgtgttggct ataagataaa ggtattttgg aagcctgtat agtaatttca
     7141 cttttgctag aaattatgga agttactata caggtttttt gtgtaaaaag atttaatata
     7201 atttagactg tattgtgtta aaataaatat ataaaaacta tattatacaa ggagagatgt
     7261 gaatttatga aacatgaatt agtacttgta atagattttg gaggacaata taatcagttg
     7321 atagctagaa gagttagaga aaacaacgta tattgtgaaa tacttccatg tactgcaagc
     7381 atagaaagaa taaaagaaaa aaatcctaaa gggataatat ttacaggcgg tccaaatagt
     7441 gcgtatttag aagattctcc aacaatatca aaagaaatat ttgaactagg aataccaata
     7501 cttggaatat gctatggtat tcaaataatg tctcatgtat tgggtggagt tgtaagaaaa
     7561 ggtaataaac aagaaaaaga atatggaaaa acagctataa cttatggaaa atcatcatta
     7621 tttgaaggaa taactacaaa tagtgtatgg atgagtcaca cagatttaat agaaaaagtt
     7681 ccagaaggat ttactatagt tgcaaatact aatgattgtc cagtagcagc tatggaaaat
     7741 gtagaacgaa acctatatgg agttcaattc catccagagg tagaacactg tttggaaggg
     7801 gacaagatac ttacaaactt cttatataat atttgtaaag taaaaggtga ctggactaca
     7861 gattcattca tagaagataa aataaaagaa ttaaaagaaa aaataggcga taaaaaagct
     7921 ttatgtgctt taagtggagg agttgattct tctgttgctg cagttcttat tcataaagca
     7981 attggggaca acttaacttg tatatttgtt gaccatggtt tacttagaaa aaatgaagga
     8041 aatgatgtag aaagaatatt tagagagaaa tttgatataa atttaataag agttaatgca
     8101 gaagatagat tcttatctaa attaaaagga gtttctgaac cagaagctaa gagaaaaata
     8161 ataggtgaag aatttataag agtatttgaa gaagaatcaa ataaattagg aaaaatggat
     8221 ttcttagtac aaggaacaat ctatccagat gttatagaaa gtggacatgg aaatgctgct
     8281 actataaagt ctcatcataa tgttggtgga atcccagaag atgtagactt ccaagaaatt
     8341 gtagaaccat taagagagtt atttaaggat gaagttagaa aaataggatt agagcttggt
     8401 atagaagaag gattaatatt tagacatcct ttcccaggac caggtctagg aataagagtt
     8461 ataggtgatg taactaaaga aaaatgtgat atattaagag aagctgatgc tgtttatatg
     8521 gacgagctta gaaaagcagg tttatacaga gaaatatggc aagcttttgc aacattacca
     8581 gatgttaaga ctgttggagt tatgggtgat gaaagaacat atgcttattt agttggttta
     8641 agagcagtta cttcttctga tggaatgact tctgactggt ataagatgcc ttatgatgta
     8701 ttagaaagaa tttctaatag aataataaat gaagttgatg gagtaaatag agtagtttat
     8761 gatataactt ctaaacctcc tggaactatt gaatgggaat aaaatataga atgccaaaaa
     8821 tccacataaa tacaggattt gaggtctctc ttacttctat gttatactgg tttgatacta
     8881 tcaatatttg ccagtatctt atggattaat aagcaaaaaa catcctgctg aatgataaat
     8941 tcagcggggt gtttttattt tgttttagaa ctcctttgct ctgaaactcg aagtatttaa
     9001 cactgatgat tttgttatct tttagttcct tttgtgtcat gctctgaaag acaaattgat
     9061 acaataaaaa agtaatttct taatcattga tggagatatg tgtttatgtt actcttgatg
     9121 gtaatttgag taatattgtt catagctttt tatattcact taattagaaa accatttggt
     9181 ataatcataa ataaacttga tgatataata gaaaagattt ataattatta gaagtatata
     9241 aaagtatttt atgtctatga aatgtatgtc tataaaacat atagatgact tttgtatatt
     9301 aatgtatgaa tattgatatt atagaagaat tgagttgtca atgatactaa atcaaatatt
     9361 tatagaagct ggagggatta tgagaaataa aataattatg ggaattactt tttcacttgt
     9421 attactaatg tctggatgtg cttcaaaaca aaccgcagaa ataaacgtat tagcaactac
     9481 tgatttacat ggagttatac cttatgagtt aacttcttat gtaaaagaag aaagaaaaaa
     9541 agataaaaat ctaactttag tagatgcagg agattttttt gatagtggag aagtgtttgg
     9601 ttctagcatg gatagttatt ttagtgctag atataaagat ggtgataata acactgaaag
     9661 ttatattgag acgcctattt caaaagatat gaaagaggta ggttatgatg ttgcagttct
     9721 tgggaatcat gaatttatat ccaacaataa attttatcta gataatatga tatctgattt
     9781 tgagaagcaa aaaataaata ttttatcggc aaatacgtat aaaaaaaatg gagatagtta
     9841 tacaaaacca tacataataa aaaatattaa tacacctgaa ggaaacgtaa aactaggaat
     9901 tttagggctt actataaaag aagttggtga gaggaaacag tggaaagatg gaaaacttgt
     9961 tgactctaag tctttagaac taaaagacca agagggttac aatggtgaat tgtatatgaa
    10021 tgatttagta gaagatgcta aaaagtgggt tggtataatg gaaaaagaga agcctgacat
    10081 tatagttgct gtggcacata caggtgagaa acctaagaaa cctaaaaacc caggaaatag
    10141 aatacaagac ttagctcaac aagttgatgg aattgatgct atagttgcag gtcataatca
    10201 tgttcaaatt gagcaacatg attataaaaa taaatcagga gaaaatgtta tagttactga
    10261 gccaggaaag catggggaat gtatttctaa gattaatttt aaattagaaa aaaacaatga
    10321 taaatggaat atagttgaca aatctagtaa attaattaaa tttgaaaaaa atcaagaaag
    10381 tgataaattt ggagaattta tttcttcaat taataaagta agtaaagata agaaagaaat
    10441 aagattatca gaagtaaaga ctcctgaatg ggataaaata tatgttttta aatctggaac
    10501 atctgcagat gatatatata aaacaatagg atataaatgg agaaatatat catctccaaa
    10561 gggtgatgac atggtgcaga ttgttgtaat gaataataat aagcctgtta gctatgtata
    10621 tggtgaagct gaagttatgc caattaatat aaaatgtgat gaatcagagt ataaagataa
    10681 tgttgtaact atttatccta ataaaaatga taaatttaaa attgaaaaag gtaaacaaga
    10741 atatagtatt aatttaaaac atatacaata gaaactttta tcttatttaa gtttagattg
    10801 tacttaaaat aaagtgtgta ttattttaaa aattgatgat aattaaaatc acaacattaa
    10861 ggaatagtac acacttttta tgctaaaaaa tagaatgtta aaaaattaaa cctttaaatt
    10921 aaagtacatt gcttatatta tccaatatta ttttcaaagg ttgtgtaata tttttatcct
    10981 tatgtaatat tgctaacatt ttattttcca tatctatccc atctaattca atcaatcgca
    11041 attttttatg taagagtgaa tctaataata aattttctgg caaaatggtt atacctaacc
    11101 ctgcttgtgc agctttaata agtgcaaaag aattaacact ttcccaaaca ggataagctt
    11161 tttggtttga aagagataat acactgtcca aagtattgcg aatggcactt ccttgctcac
    11221 gtaacaataa cggaaacgtg cataactgtc gtagagataa ggcttgttta gtattggtgg
    11281 tggttatgca atcatacttt taatccaaga gaaagtagta aatcaaactg gatggataag
    11341 tgagaaaatg tttacagata ttataactat ttcacaaatg acaccagggc cactggctgt
    11401 taatacttct acttttgttg gacttcaaat tggagggttt tggggagcag ttgcagccac
    11461 tttaggttgt gtattatgtg gtgtagtaat atctctattt ttatataatt tttttcaaaa
    11521 gcatcagaac tctacttata tatttgaaat attaaatggt ctaaaatcag cttcattagg
    11581 tttaattatc tcagctgcgg ctacaattat aatccttgca ttatttggga cgaataaagt
    11641 gtacttcaat tctatatttg agatgctaaa ctggactgca ctaataatct tttttgttac
    11701 actactgata gtacgtaagt ggaaaattaa tcctattgtg attataataa taagtggtat
    11761 actgggaata tttttttatt tataagtttt tggtaagtag aggatatcta tttttttat
//
LOCUS       DAGSHT010000104        11663 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGSHT010000104 DAGSHT010000000
VERSION     DAGSHT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.106"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            159..1334
                     /locus_tag="MJB72_002401"
     CDS             159..1334
                     /locus_tag="MJB72_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8263489.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1507..2904)
                     /gene="asnS"
                     /locus_tag="MJB72_002402"
     CDS             complement(1507..2904)
                     /gene="asnS"
                     /locus_tag="MJB72_002402"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBY8263490.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(2981..3214)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3383..3577)
                     /locus_tag="MJB72_002403"
     CDS             complement(3383..3577)
                     /locus_tag="MJB72_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBY8263491.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3850..5523)
                     /locus_tag="MJB72_002404"
     CDS             complement(3850..5523)
                     /locus_tag="MJB72_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBY8263492.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5533..8931)
                     /locus_tag="MJB72_002405"
     CDS             complement(5533..8931)
                     /locus_tag="MJB72_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBY8263493.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9099..10295)
                     /locus_tag="MJB72_002406"
     CDS             complement(9099..10295)
                     /locus_tag="MJB72_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBY8263494.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10316..11137)
                     /locus_tag="MJB72_002407"
     CDS             complement(10316..11137)
                     /locus_tag="MJB72_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBY8263495.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3749 a         2101 c         1338 g         4475 t
ORIGIN      
        1 aagttactcg acattatcta ataaatttta tctatcacaa ttttagattg ttacacacaa
       61 tatattttga tataattaaa tcataaacga agtattccaa atatctaatg atagaaagga
      121 atacttattt ttatgcttta tttttaatgg aggcttatat gaacttagaa aaaataaaaa
      181 aattttttgg atttcctcta gacaaaaaac acaaagacaa tgttgagaaa gaactctgca
      241 attatcttta tcatacgggt aaaatcattt gcataataat agtaatatct caaataatta
      301 tgatgatatc aatctcagct agagaaggag gcccttttgt cacaccaaga cgccaatggt
      361 atttttatat gtatgtaaca ctattttgtg tatcttctgt ctttatgttt gtattttctt
      421 acctttggaa taaaaaaaag gattgctgta cgttttatat aaatttatgg ataatttatg
      481 tgactttttt ctgcctatgg ggttgtgtaa ttactctact tgaccagtta ggtggaaatg
      541 acctaagtgt atttagttat attatgttag cagctgcagc tttctcagta ttaaaatttg
      601 gagaatcagt aatagttttt ggaggatgct ttttgtttct taatttattc ttacctggga
      661 ttcaaactat accaggcaat gagtttagta atattgtcaa cacatttttt ataacatccc
      721 ttgccatttt tgtttctaca atgctatata gaaatcgtgt atttacttac tatgataaac
      781 ttttaattag tcaacaatat gaagaaatat ctcatataaa taatttactt aacgacaaag
      841 ttatgacaga tgaccttact aaaatgaaca accgtagata ccttgaagat attctacaaa
      901 aaaaattaat cagccatgca aatcaagaac ctataactgg aatgatgata gatatagatt
      961 attttaaaca atttaatgat aattatggac atcaaaaggg agactattgt ctacaaaata
     1021 ttgccaaagt aattttagat tttacaagtt ctgaaaatga acatgctata cgatatggtg
     1081 gtgaggaatt ttttatatgt atttttaact gtaatgaggc agatgctaaa cttaaatcag
     1141 aatgtttacg aaaaattata gagtcctgca attttgagcg tgatgatgtt cctttaaaat
     1201 gtgtaactgt tagtattgga gtttatacaa agaaaattgg aaagacaata acattaaatg
     1261 agtttataag taattgtgat aaggccctat atactgcaaa agaacaagga cgtaattgtg
     1321 ttgcatttta ttaaaaggtt acatactatt acataaacct ataagtatta tatcgcaaaa
     1381 taaaagtaat caaattatat atttatagta caataaaaat aacttgcata tctctaaaat
     1441 agataaatat gcaagtcatt ttagtgttta tacttaaagt tatataagtt ttattatata
     1501 taattattaa aattcagcat ttttaggagt tcttgggaaa ggtataacgt ctcttatgtt
     1561 agacatacct gtcatataca taattattct ttcaaatcca agaccaaatc cagagtgagt
     1621 agcagtacca aactttctaa gttctaaata ccaccaataa tcttcttctc ttaatcccat
     1681 ttcattaatt ctatctaaca atacatcttc tctttcttct ctttgagaac ctcctacgat
     1741 ttctccaact ccaggaacta gtaaatccat tgctctaaca gttttaccat cttcattcat
     1801 tctcatatag aaagctttta tatcttttgg ataatctgta acaaacactg gacaattata
     1861 tatttgctcc gttatatatc tttcatgctc tgtttgaagg tcacatcccc actcaacagg
     1921 atactcaaat tcatgcccag attcaattaa taattccact gctttagtat acgttattct
     1981 aataaactca gagtttacta tcttatttaa tctttctaac aatcctttat ctataaaact
     2041 attaaaaaat tccatttctt caggagcatt ttctaataca tagcttataa catattttat
     2101 catatcttct gcaagttcca tgttatcttc aaggtctgca aaaactattt ctggctctat
     2161 catccaaaat tctgaagcat gtcttcctgt ataagagttt tcagctctaa atgtaggtcc
     2221 aaaagtataa acgtttctaa aagaaagtgc cattatctcg gcatttaatt gaccactaac
     2281 tgttaaatta gcctcttttc caaagaaatc ttgagtatag tctattttgc catcttcagt
     2341 ttttggaata tcatttaaat ccatagttgt tattctaaac atctctcctg caccttcaca
     2401 gtcactacct gttatgattg gagaatgtgc atatacaaag tttctttctt ggaagaattt
     2461 gtgtattgca taggcagcta ttgatcttac tctaaataca gctgaaaaag tattacttct
     2521 tggtcttaaa tgtgctatcg ttcttaaata ttcaagcgta tgtctcttct tttgtagtgg
     2581 atacgaacta tctgattccc cttctacaac tatgcttttt gcatgtattt cgactggttg
     2641 tttagcccct tctgtagata ctaactctcc ctctacaagt atagatgagc ttattgctaa
     2701 ttttcctacc tctttaaaat tttctacttt ctcatcaaat actatctgca tatttttaaa
     2761 gaagcttcca tcatttagct ctataaatcc aaaattttta gatactctag atgttcttat
     2821 ccatccagct acttttacag tcttccctat gtactgttct ttttctctgt acaattcttt
     2881 tactgttata aattcctttt gcattttaaa tcctcctagt ataattgtta ttaatttctt
     2941 ttaaatataa tttcaataaa aaaagccctt catccctata taaagggacg aaagactatt
     3001 actctcgcgg taccacccta gttgtcataa tgacctctta gttaagcaat gcttaaagcc
     3061 tataacggtg ccttccgtct aagtctactt actaaataag ctttcgatta gaaactccaa
     3121 gatgttcttc aatataaacc tcgtattgag ctttcaccat cctcaactcg ctagaactac
     3181 attttatatc tactcttctc ttcacagtat ttatctttta ttatttctgc taattaacaa
     3241 aattatacca atttataaat ttttagtcaa taaatttata taattgatac tgattgttat
     3301 ttaaaatata ataattctac cttacataat gataataaat acttatcttc attacttctt
     3361 tattacttca atttccaaaa ttttataatt tacttccatt gtcaacccag tcttttgctt
     3421 cttcgacacc cttaattgaa ggtattgaaa ctttactatg aggttttaat ttttcaacat
     3481 cagcccatgc tgctgtcgac tcatttccaa ctaaaagtga acttttaagt cttttattat
     3541 ctttaccttt taatgctctt gcctttttac tttccaaaac tatcacctct attatttatt
     3601 ttgtaatagt tttagcaatt ttacaaataa aattcataag tctaaattat aattattttt
     3661 aaattaacat catatctttt caatacttaa tttataccta tttatataca aaatatttac
     3721 tttgtgtatt caaaatataa aactgttttt taattttcat atttattttt tatgctattt
     3781 ttctactcta tctaaagtat ttgatacaat taaactaata atcaattata attttacata
     3841 ggttcttatc tatagagtat ttgctatctg ttgaatcgta ttttttaaat ttaaaatccc
     3901 atatccctgc gatacatttg gatatgtata tatctcaagt tttgtagcac ccaatattaa
     3961 atatgttttt aaaacttgtg taaacaatga taatctcggt acattatcct gtttttctaa
     4021 gtattccatc aataaggcta aaacacctgt gactatagaa ctgctcactc ctgttcctgt
     4081 tcctgtatta tatgttccat ttttatatgt ggatattata tctacacctg aagctactat
     4141 atctggctta attcctcttc ctctaattgg tcctttcgaa gaaccaatcc acatactatc
     4201 agtcttattg ttaaatgtac caactgtaat tacatcatca ctagcagcat acatagttat
     4261 agtagctact gaatcagggt ctaaaaacct tgtgtctgga gctatcaaat ttttatttgg
     4321 caagtatatg tcatattctc catttattat aagttctgaa gtcaatctta aaatccatac
     4381 tccaggtttt atatctctta atcttatctc caaattttct tttccagagg taatatatgg
     4441 ataaataaat ctcattgcat aagtagtatt ttctaagtta aattttcctc tatatatata
     4501 aaagtctgga gaatacctta tatcatgact aacttcacct gaaggagata ttatctgtgc
     4561 tcccacttta tctggaccat tcgtatttag tgttatatca agagcatagt catctccatc
     4621 ttgtattatt acatcttgaa cttctcccac actgctaaat ctgccagaat aatgaatatc
     4681 tgtattccct tgatttcctg caccactcac tactaccaca ccagcactac tcaatatatt
     4741 aaaagtatct agtattgaag tagttgctac agcacttgac ttaactccaa tagtcaaatt
     4801 tattatcagt ggtttatttt ctgttcttgc tatgttagta acatacgtta tagctgctaa
     4861 aaaatctgaa acactgtaat ttatccttcc agcataataa gtatctgtat atgattttaa
     4921 tttaactact attaagtctg attctgtagt aatacctctg tactgagaat ttatccttcc
     4981 atttccaact aatataccag aaactaaagt tccagtacct atattatctt gactcaaact
     5041 cccatcattt ctatttatgg ctatatttaa ctcacttctt gtaaattcac ttccaaaaat
     5101 aaaaccttct ggaggtggat ttgtatttgc ttcttggtcc cataaatata aaactttact
     5161 agttccatca tcatttataa aatcaggatg caaataatct atcccagaat ctataactgc
     5221 taataaaatt cctctccctg ttatatcatt atatggattt tcataaatat aatcagtttc
     5281 agctgctgtt gtaatggttt ctccattatt tacattatta gttatttcaa ttagactgct
     5341 cattggttca gattcttccc accaagcaac ttgtattata ttatttaaaa tagtttcatc
     5401 aaaatctaaa ggtacatata ttactgcaag ctgactattt aaaaccatat acctatttat
     5461 tccattttct tgtagggcag attctatatc accttgataa attatacaat aagatttttc
     5521 cataaatccc tcctatctta aaacatcaaa cattcctcta acatccaaga gcccatatcc
     5581 agaatttgta tttggatata ctgtgtttga atcttttcta gcaccagctt gcataaaagt
     5641 ttttattttt tgcacatatg cttggtttgg atatctaccg tcaacaaaag tatattgaaa
     5701 atacattgca gcagcaccag ctgcatgtgc acttgctgct gctgtaccag ttatagttgc
     5761 atacgtgttc ccaggataag cagctattat attgacacct ggtgctacta tatctggttt
     5821 taacctgtct tcaatagttg gccctcttga tgaactttgc cataaactac cattaatagt
     5881 attgtaggct cctatggtta ttaaatcatc ttgaatcgca ggataattta tagtgtaaaa
     5941 agggtctacc tctctaaatc tagttccact ttttaaaagt tctctatttg gaaggtataa
     6001 gttatatctt cctgttataa tataaactcc tactaatcga atcttccata cacctctttt
     6061 tgcattcttt aaagtaacat tagtaaattg ctgtccagaa aaagttgttg ggtatatata
     6121 tgttatagaa tattcagtcc cctctaaatc aaataaacct gttactttat tataatttga
     6181 aattccaaca cttttacttt cttcaccagt gggagatact attattacat cagctttatc
     6241 tggtctattt aaccaaagtt ctaatgataa ttcttcctcg tcttcattta actctaattc
     6301 aacttcaaca gaacctccaa catatggtat tattccagat gtatgtgtct gtgtattgcc
     6361 ctcatttcct gctcctgccg ttatacataa ccctctagta aagaacgctt tttcactatt
     6421 actcctattg gttaaacctg ctaggctact ggtacctaaa gacatattta taactaatgg
     6481 tcttccaagt tcaaaagctt ttttatatgc atactgagat gcagcaaaca acattgcact
     6541 attataaaaa tcatctatct tgccgagttt tattattatt agctcagaat cttctgctat
     6601 acctgcatat tcactattta catttccaag ccctgcacat atcccactca acatagttcc
     6661 ttgaccaact tcatcctgag atagactagg gtcattttct gcgatagctc gatttatatc
     6721 ttctcgtgta tattctgtcc caatataaaa accatcagga ggagttcctt cttttgtttg
     6781 gtcccataaa tatactattt ttgaagtgcc atctggataa ataaaatctg ggtgtagata
     6841 gtctattcct gtatctgcaa ttgatattag agtccctctt cctgttatat taatattagg
     6901 attattttta aagaaattta cgcccatttc ttctgttgca actacacctc caaaagtacc
     6961 tcgattaatt tctccaagta atgtcatctt tgtagtcgat acagttctta tgattgaagg
     7021 aagttgcaaa actcgttgta tgctattata gtctgtatta tttatagata gtacacctaa
     7081 tgaatcactt atcttaaaaa acctttgact catacctaac ccagctagtt cttcttcaaa
     7141 ttcaggagta tggactatat ctattcctga tagtattcct gattgtctac ttgcattttt
     7201 acttatataa atttcattct ctaagtcatt atgtatttgc atattataac catctataaa
     7261 cttataacta ttttcctcat ctttaaatga cttattaata cttctatatc caatatcctc
     7321 tgtttctaaa tcttgattta tattagatag agtgtacaaa tctatatctg ataaatttaa
     7381 aaaaccaaat cctgatgaat tatttggata agattgatta cttaatctcc ttgctccctt
     7441 tagaagtaat gcccttaatt tctgtgaata caaaaataaa tcatttccat ttacaattcc
     7501 ccattccata aacaatgaac aaacccctgt aacatgaggt gtagccatgc tagttccagt
     7561 taatgctcca cttgtacctc ctggtaaaaa agatactata tcttcacctg gagctaataa
     7621 atcaggttta aaaacaccta attgtgtatc tccttcacca gaaaatatag atactatatc
     7681 tgttcttgaa ttaaaactac ctactgttat aactctactt gctgttccag gaactgttac
     7741 agtaagctct tgagtaggaa tcagaaatct agtatttcta ttcagacctt cagaagttgg
     7801 caaatatata tttacattac cagttactat atctattggt tcaaatacaa ttttccataa
     7861 cccaggagtt atctgtgtat ttgaacttaa ttgtagagta actcttcttg tgagagaata
     7921 cggtgctata gggtaaaaat aaccagttat tctagtttca cccaaagtat tccttatctc
     7981 acctgaagtt agagagattg cttgtgtttg attatttgat gggtttacca aatgtacact
     8041 aaaatcatca acaaaatctg gccatatatt tatgtttaat atacgctctc cttccccaac
     8101 tataaactca acttcttctg taatattatt ttgaagccta atccttttat gacctccttt
     8161 atctgcatta tttccagctg ctacaactat attgtttttc caaaataaac acatatcatc
     8221 tatatattgt tcaaataaag atgttcctct atgtgaacct tcattacttc cataacttat
     8281 atttaatgta acaggcattc ttaattctaa tgccctatct aaaataaatt taattgccct
     8341 cataaactct gtacttctcg aaaaaatatc ggtctgtata tttccaactc taaccactat
     8401 tattctggca tcacttgcaa tagttgcaca aatacctgca acatgtgtcc catgtaagct
     8461 agttgttgaa ataggtatat acatacttcc atctattgca ttatttatat cttcattagt
     8521 atataatgtt ccttctctaa aaccttctgg tggatttcct tgaatagatt ggtcccaata
     8581 atataatatc ttagaccttc catcactatc tctaaacaca ggtagagtat aatctattcc
     8641 cgaatcaatt ataccaatta tagttccttt tccagttaaa ccagttctat ttttaaatcc
     8701 tgttattcct gtacttgaaa aactttgcac atcctgagtc tgtaatataa aaggcttttc
     8761 tataaattca atttctggat aagttaaaag tatatttaca tcttcttcat tacttgatgt
     8821 tattattgca tatgatgaat taagtatctc tactgataca cctaattctt cttctaacct
     8881 caatatatct ccattgtatt ttacaattaa ttcataattt attataatca cctcgacttc
     8941 taaaattatt attaatgtat atgttttgtg tgagtctaaa atgtattaga ctatataatt
     9001 ttttaagttt cagttataca atttataata gatatattaa aaaaaataaa taatgatggt
     9061 acaattttga ataaatcata ccatcattta tatttacttt atcttgaaac cattaaattt
     9121 ttcaatgctt tctcaagacc ttttacagtc aatggaaaca tattaaattc actttgtatc
     9181 attctttgag tttgatacca ataattaatt gaatcccatc catctctaag ttctggattc
     9241 atccatacaa ttttactgta ctttctctta aaccttttaa gccactctat accaggtgtg
     9301 taattatatg gaccacgata gttaccacca atatgaagca actcagaagg cgacatagaa
     9361 gcatctccaa caattataac tttatagttt ttatctatat tttttaatat ccattcagta
     9421 ttaatagatt tacttaacca acattcaggt gttgtaaata atctatcata aatacagtta
     9481 tggaaataat atatctttac atctttaaaa tgatttgatt tacttacaga ctgaaataat
     9541 gtattacata aagtgctata ccctctcatt gaaccacctg aatccataag caataaaagc
     9601 ttcactgtat tttttcttgg cttctcaaaa aataacttta aatatcctgc attattacaa
     9661 gtctcttcaa tagtcttatc caaatcaagc tctgtctttg gagcatctat tctggtagaa
     9721 aattgacgaa gtctctttag tgcaacttga aattgacgca tatttaaaac ttcatcatct
     9781 ctaaaatctc tgtattttct ttctcctgca acttcaagaa cagctctacc ataagtagaa
     9841 tttccaccta tacgtatacc agtttgccct tttcctgaat gtcctaactc tgaagtgcct
     9901 cctgtgccta tccaataatt accaccatta tgaggagagt cttgttcttt tattcgttcc
     9961 tccaatttag ctcttaattt atcaagattt atatttggat tttgttctaa tctttcaaac
    10021 tcttctaaat ctatatctgg ttttttaagc caattcataa tctcttctgg aatttcttct
    10081 tggtgttcta tacctttaaa atactccaaa aacactgcat caaacttatc aaaatcactt
    10141 tcacttttta ttaaaatcat cctgcaaaga tagtaaaaat ttgaaaagtt tgaatagcac
    10201 agtcctttat ccaatgcttc aattaaagtc atccattcat ttaaagatac atttaatcct
    10261 cttgacctca ataaataaaa aaattcaata aacataatta tccagctcct attaactata
    10321 agtgtctttg catagattca atatcctcat tattcttaag tagtattcct gcaaaaggaa
    10381 cttctttttc tattttttca ataggcattc cactcaaagt taatgcttgt atccagtcta
    10441 ttaactctga tgtactaggc tttttttgta tgtcctttaa acttctaatc caataaaaag
    10501 tagtcattac ttgttccaat aggtgttctt ctactttatc aaaatgtact ttaactattt
    10561 cttccatcat gtctctatca ggaaattcta tataatgaaa tatgcatctt cttaaaaaag
    10621 catctggaag ttctttttcc gcatttgaag ttataataac tattggtctt tgtttagctc
    10681 ttacagtttc ttttgtctca tttatgtaaa actccatttt atccaattcc caaagcaagt
    10741 catttggaaa ttctaaatcc gccttatcta tttcatctat taaaagaatt acttgttgat
    10801 ttgagctaaa ggcctctcct agtttaccat attttatgta cttgctaata tcatctacac
    10861 cttctcctcc aaattggcta tcataaagtc tttgaacagt atcatataca taaagccctt
    10921 cttgtgcttt tgtagttgat tttatattcc aaattactaa atcttttttt aattcatttg
    10981 aaattgcttg tgctagcata gttttacctg ttccaggttc gcccttaatc aaaagtggct
    11041 tttcaagagc aatagcaaca ttaactgatg ccatcaattc tggtgatact acataatcac
    11101 tacttcctct aaagttagat atctcttttt ttatcataaa tcctactcct tttaaacagt
    11161 attttctaaa tgtatataga ctttattact atttttgata taaaaataat aatacaatac
    11221 atttgtatag tattattcta aaattctata tctaaataaa atcacctcta aaatatttaa
    11281 tttttatctt tatttgtcat aattaaatga aatatatatt ttaaagaata ataaaagata
    11341 gcttattgta caaaatttaa attataaaaa cttagtttaa tcaaaggcac cacttttttt
    11401 cattttagat tatatataaa cacaatataa ataattttta ttattttagc tataaacata
    11461 aagttttaac gtatttataa tttcatatta atcatactat attaatactg ctttttattg
    11521 tataataagc tataaatatt aactttaact tagatttaag tatatctaaa tttaataaat
    11581 ataagaaagg ttgtgttatt ttgcaagata aaaaaattta tctaaatatt gtactcactg
    11641 tagttgtttc atatatcttg att
//
LOCUS       DAGSHT010000105        11630 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.107, whole genome shotgun sequence.
ACCESSION   DAGSHT010000105 DAGSHT010000000
VERSION     DAGSHT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.107"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            48..680
                     /locus_tag="MJB72_002408"
     CDS             48..680
                     /locus_tag="MJB72_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBY8263496.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            819..1358
                     /locus_tag="MJB72_002409"
     CDS             819..1358
                     /locus_tag="MJB72_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBY8263497.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(1534..1704)
                     /locus_tag="MJB72_002410"
     CDS             complement(1534..1704)
                     /locus_tag="MJB72_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8263498.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            1989..3056
                     /locus_tag="MJB72_002411"
     CDS             1989..3056
                     /locus_tag="MJB72_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBY8263499.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            3226..5127
                     /gene="cooS"
                     /locus_tag="MJB72_002412"
     CDS             3226..5127
                     /gene="cooS"
                     /locus_tag="MJB72_002412"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBY8263500.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            5120..5593
                     /locus_tag="MJB72_002413"
     CDS             5120..5593
                     /locus_tag="MJB72_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY8263501.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            5568..6794
                     /locus_tag="MJB72_002414"
     CDS             5568..6794
                     /locus_tag="MJB72_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY8263502.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(6933..8120)
                     /locus_tag="MJB72_002415"
     CDS             complement(6933..8120)
                     /locus_tag="MJB72_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8263503.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            8393..9217
                     /locus_tag="MJB72_002416"
     CDS             8393..9217
                     /locus_tag="MJB72_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8263504.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            9346..10611
                     /gene="gluD"
                     /locus_tag="MJB72_002417"
     CDS             9346..10611
                     /gene="gluD"
                     /locus_tag="MJB72_002417"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBY8263505.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            10824..11414
                     /locus_tag="MJB72_002418"
     CDS             10824..11414
                     /locus_tag="MJB72_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY8263506.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         4534 a         1253 c         2112 g         3731 t
ORIGIN      
        1 ttttattgtg aatcaaacaa aaaagaggac acttttagga ggtgaagatg ttgggaaata
       61 aaaatatatc aatggcagtt ataagaaggc tcccaaaata tcatagatat cttggagact
      121 tattagatag ggatatacaa agaatatctt ctaaagaatt gagtgatata atagggttta
      181 ccgcttctca aataagacaa gatttaaaca actttggtgg atttggacaa caaggatatg
      241 gttataatgt agaagctctt catactgaga taggtaaaat tcttgggttg gatcgaccat
      301 acaacgcagt tcttgtagga gcaggtaact taggacaagc tatagccaat tatgcaggat
      361 ttagaaaagc tggattcgag ataaaagctt tatttgatgc aaatcctaga atgataggtt
      421 taaagataag agagtttgaa gtattagatt cagatacttt agaagacttt ataaaaaaca
      481 ataatataga tattgctgta ttatgtatac ctaaaaatgg agcacaagaa gttattaata
      541 gagttgtaaa agctggaatc aaaggtgtat ggaattttgc acctttagat ttagaagttc
      601 cgaaaggtgt tatagttgaa aatgtaaact taacagaaag tttatttacc ttatcgtatt
      661 taatgaaaga aggaaagtag acaacttaaa catattaata atgagcagtt gtgaagagta
      721 ttgagaaagt cagaagattt aatattaaat cctctggctt tttgaaatat taggataaaa
      781 atttaaataa tgtatggaaa taaacaatat taaggagagt ggtttttgtg gaggaattat
      841 ggaaaaaggc aattgaattt catggtcatg aatgtccagg gttagctata ggatttaaag
      901 cagcacttgc agcaagagaa tatttaggat gtaaacagtc aggagatgag gaaattgtat
      961 gtatttctga aaatgatgct tgtggaatag atggtataca agtcgtgcta agttgcacaa
     1021 tgggtaaagg aaatttacta cttaggatga ctgggaaatc agtatatcat ttctttaata
     1081 gggaaaatgg aaaatctata agaatatatg taaagtcaaa gccatttcaa atggatagag
     1141 aaggatacaa aaagtactta ttagaaagtg attttgaaga aatatttgag attacagata
     1201 ctaagttaag acttcctgaa aaagcaagaa tatttaaatc tcttaaatgt gaagtgtgtg
     1261 gtgaatttgc atctgagcaa tatataagga ttgaagaagg aaagaaagta tgtcttgatt
     1321 gctttaataa atatgaaaga ttttatgagg agtattaaaa ttataaatta gattaaagtt
     1381 aaaagtatta gatatgtgct aacagtattt taagatgaat taggtagcaa cataataaag
     1441 tatataaata atattaattg aatcatacat aattaaaaag aggctgatta gtatttataa
     1501 aataaaatca gtctctttat gaataacgaa ttattattct ggttgaggtg catctacagg
     1561 acaagctcct gcacaagaac cacagtctat acaagaacca gcatctataa catatgcgtc
     1621 gtctccagct gatatacagc taacagggca ttcagcctca caagcaccac agcttataca
     1681 agcgtcagta attttatatg ccattttgaa atcctccctt ttatatgtat tgtaattcaa
     1741 taacattata acaaatatta ttgaatataa caattaaatt aattctacaa caaaaacttt
     1801 attatgatat tcttgtttta aagttttaca ataaagttta atctgcttat atttttattt
     1861 attttaaaga agtttatttt aaaaataaaa ttaaatttaa aaagttatgt attgattata
     1921 tttaaaaatg ttatactcta aaaaagtata ttttatttaa aaatttatta atacaaagga
     1981 gatttactat gaaaaataaa ttagtttaca tatgtattat gagtctattg ctatttgtag
     2041 taggatgttc taataatcca aataaggaaa gtaccattaa aaaagactct aagaataacc
     2101 aaagccaagt taaagaagaa aaaagagaac ctgtgaaaaa agatataact ataagtttcg
     2161 ctggtgatgt aactatgggg aactataaag gttcatcata ttatggcagt tttgaccatg
     2221 aatttaaaag acaaggagaa gattacaatt attttcttaa aaatgtaaaa cctgtatttg
     2281 aaaaagatga cttaacagtg gttaatttag aaggtccact tactacagcg tcaaatgcaa
     2341 aagaaaaaaa atttgcattt aaaggaaagc ctggatatgt aaatatttta aaatctggaa
     2401 gtgtagaggc agtaagtatt gcaaataatc attcagaaga ttattttgaa gaaggaatga
     2461 aagatacaca gtttatattg gacgagaata aaataaatta ttttggatta gggaaagatg
     2521 ctgttgtaga tgtcaaagga attaaagtgg gtctattagg gtataatgga ttatcaactg
     2581 aatataatga aaaaaattta aaacaaatgg aagatgatat taagtcctta aaaaagaaat
     2641 cggatgttgt tgtagtgtac tttcattggg ggatagaatt agattattat ccagataaaa
     2701 aacaaaaaga ttttgctcat tatgctatag acaagggagc agatttagtt atggggtctc
     2761 acccacatgt gattcaagga atagaaaagt ataaaaataa gtatatagct tatagtctag
     2821 ggaatttttg ctttggagga aataagaatc cttctgatac agatagttat atatatcaac
     2881 aaacctttac tttttcagat aataaattaa cttcaattaa agagccaaat ataataccaa
     2941 cttcaataac atctagtaac tccagaaata attatcaacc aaaaatattg gatggaagtg
     3001 agaaggatag agttttaaat aaacttgaaa aaataagcaa agatttaaat aaataaaatg
     3061 taaaaaaaaa taaatatata tatttatttt aataatttat attttgttta gggtatggta
     3121 aatcgtggta ttaaataagt agattttaat agttaaatta tagtcaatta taataaagct
     3181 atttaatcta cttttttaat ataaaaatat aagggggaat ttgtaatggc ttgtaacact
     3241 tgtaaaatgt gcgaaagtgc agacaagaaa ttagaaagtt ttgtagcttc aaaggatgtt
     3301 gaaacagcat ttcatagaac agaagatcag aaagttaagt gtggttttgg acttcagggg
     3361 gtttgttgta gactctgttc aaatggacca tgtagagtaa ctcctaaatc accaagaggt
     3421 atatgtggag cagatgcaga cactatagta gcaagaaact ttttaagagc agtagcatca
     3481 ggagcagctt gttatttaca tgtggtagaa aatacagcta agaatttaaa aaatgttgga
     3541 ataactaaag gtgttgttaa aggagaaaaa acattaaatc aattagcaga gatgtttgga
     3601 atagaaagtg atgaaaagta tgataaatgt ataaaggttg cagacaaagt tatcaacgat
     3661 ttgtataaat caagagatga taaaatggaa ttagtagaaa aaatagctta tgcacctaga
     3721 gtgaaaaagt ggaaagagtt aggtataatg cctggtggtg caaagtcaga agtttttgat
     3781 gcaatagtaa aatcatcaac aaatttaaat agtgacccag tagacatgct agttaattgt
     3841 ttgaacttag gtatatcaac aggattatat ggtcttacac taactaatct tttaaatgat
     3901 gttatgttgg gagaaccagt tataagaatg gctcctgtag gatttaatgt aatagaccca
     3961 gactacataa atataatgat aacaggccat caacattcaa catttgcaaa tttccaagat
     4021 agattaaaag atgaagatgt tataaaatta gctcaatctg ttggtgcaaa aggctttaag
     4081 ctagtaggat gtacatgtgt tggtcaagat ttacaattaa gaggagaaca ttatcaagaa
     4141 gtatttgctg gacatgcagg taacaatttt acaagtgagg ctgttttatc aactggagca
     4201 atagatatag ttctttcaga atttaactgt actataccag ggcttgaacc aatagcagat
     4261 aaatataaag taaaaatggt ttgtttagat gatgttgcta aaaaagcgaa tgcagaatac
     4321 ataggtttag acagaagtaa attagatgaa cttagtaaca ctttaataga aaaagcattg
     4381 gagtcttata aggagagaag aggaagtatt gaaatagata taccaaaaga ccatggattt
     4441 gaacaatctt taactggtgt aagtgaaaag aacctgaaag cgtttttagg agattcatgg
     4501 aaacctctta ttaatttaat tgcagaagga aaaattaaag gtgttgcagc tgttgtagga
     4561 tgttcaaata tgacagcagg aggacatgat gtaaatactg ttgaacttac taaagaattg
     4621 attaaaaaag atataatagt actatctgca ggatgttcta ctggaggatt agaaaatgtt
     4681 ggtcttatgt cacctggagc agaagaatta gcaggagaaa acttaaaaga agtatgtaag
     4741 actttaggaa taccaccagt attaaacttt ggaccatgtc tagctatagg aagactagaa
     4801 atagtagcaa cagaattagc agctgaactt ggaattgacc taccacaatt accattagtg
     4861 ctttcagctc cacaatggtt ggaagaacaa gcattagctg atggagcgtt tggtcttgcc
     4921 cttggcttac ctcttcattt agctttacct ccatttataa caggaggaaa attggtgaca
     4981 gaagtattaa cagaaaaatt aaaagactta acaggaggac atgttatagt gaatccagac
     5041 cctaaatctt cagctaatca attagaagaa ataataatag atagaagaaa aaatttaggg
     5101 ttaaaggatg tggaatgcaa tgcatagaat ttttataaat aaagatttat gtacaggatg
     5161 taagagctgt gtactagcat gtatgctaaa acacaataaa gattatgaca tgtacactct
     5221 tgatttagaa aatatagata atgatagtag agggcatata gaattagata gtaaacataa
     5281 taatccagtt ccaattcttt gtagacactg tgatgaacct gaatgtgtat tagcatgtat
     5341 gagtggtgca atgcataaag atagtgaaag tggaatcgtt tcatatgatg aagagaaatg
     5401 tggttcatgt tatatgtgtg taatgtcttg tccatatgga ttattaaagc cagatgatag
     5461 aagcaagcag aacatactta aatgtgattt atgtaaagat gaggagtatc ctagatgtgt
     5521 agctaattgc ccaagtggag caattgaatt acagaaggag gaaaatgatg aattatgtag
     5581 tgttaggggc tagtgcagct ggtataaatg cagtaaaaac attaagagaa ttagataaag
     5641 attcaaatat agtagtaatt tctaaagatg aaaacgtata ttcaagatgt atgttgcatc
     5701 acgttatatc tgaacacaga attttaaaac aaataaattt tgttgatgaa gattttatgg
     5761 agcaaaataa tgtaaaatgg attgcaggta agactgttaa aggtatagac ataaataaaa
     5821 aggtagttca gacagaagac attactgtaa attatgataa acttttaata gctacaggtg
     5881 catcttctgc tattcctcct ataaaaaatt taagagatgg aaattttgta tattctgtga
     5941 gaaacataga tgatatttat aaaataaaag aaaaagcaca aaattctaaa aatgtagtta
     6001 taataggtgc tggtttagtt ggtatagatg cattagtggg attatttaaa tatgagcagt
     6061 taaatatttc tgtagctttt atggaaaagt acattttaga tagacaattg gatgaatata
     6121 cagcatcagt gtatgaaaat aagttcaaag aaaaaggtgt aaaattttac ccaagtgcat
     6181 caatacaaga aattgtattg gataacagta aaaatgttac aggtgttgca ttttcaaatg
     6241 gtgaagtttt agatgcagac atggtaattg ttgctacagg ggttaaacca aatgctgact
     6301 ttttagatgg aacaggaata gaatatgata gaggtataat aatagatgat atgtgccaaa
     6361 caacacaaaa agacatatat gcagcaggag atgttgtagg taaaaatgct atttggcctc
     6421 tggcagtaaa gcaaggaatt gtagctgcct ataatatggt tggaaaagat aagaagatag
     6481 aagatgaatt tgcttttaaa aacagtatga atttcatgga tataccaact atttctatag
     6541 gaatgaatac acctgtagat gatagctata aagtgctaac tagacatgga attaatgatt
     6601 ataaaaagtt tgtttttaaa gataatgtaa tctatggagc agttatacaa ggagatattt
     6661 catatgtagg tgtattaact tatttaataa aaaataaagt tgaaatatat gatttagaaa
     6721 ataggatatt tgatatatgt tatgcagact tttttaatat aaaagaaaat ggagaatttt
     6781 gttatagtgt atagtatata cgtggagtaa ggtttataaa tacagtaaat caataaatag
     6841 agttattcta aaataagaaa aatgacaaat cttatttaga aatagctcta ttttttgtaa
     6901 ttaaatttaa attatattca atttgtaaaa tattattttg aacaattata catatactct
     6961 ctagttagag gtaaatcatt ttttatacct ttagtgaaaa gtatttgatg tatatctata
     7021 ataccagtgt tgaaagcagc agcacaagaa caaaggtata aatcccacat tcttataaac
     7081 ttatcatcaa acatttcttt aatctttgga agattctcag agaaattttt ttgccagtat
     7141 agtaaggttt ttacataatg taatcttaga ctttctacgt ctatagtatg atatttgtaa
     7201 tcactacaaa tatgtatcat ttctcttagg ctaggaataa ctcctcctgg gaaaatatat
     7261 ttttttatcc aagggtctcc ataagattcc tgcaatccac ttatatagtg aagtaaaaac
     7321 aatccaccat cttttaaaac agcatttacg tttttaatga aaagttcata gttttctcta
     7381 ccaacatgtt caagcatccc aacgcttaca attctatcaa acataaggtc tgattttttt
     7441 aattctctat agtccataag tcttacttca agatagtctt gaaggttctc ttctttaatt
     7501 cttgcgttaa attccttaaa ttgctcttcg ctaagtgtaa ttccaacgcc gtgaatttta
     7561 tatttttttg cagcttctat tagtaaaaat ccccaaccac aaccaatatc gagaaggctc
     7621 ataccttcct ctaaatttag tttagttaat atgtggtgaa ctttattgac ttgagcatca
     7681 taaagagtat cattatcatt tctaaagtat gcacaagaat aactaagtgt cttatctaac
     7741 cataagctat aaaaatcgtt gccaatgtca tagtgggcat gtatttcttt cttttgatgt
     7801 ctcattgaat ttgatgagtg caatagatgt tttacagctg aattatcaat attgaatttg
     7861 tctatttgtt ttaaaagtag atttaaagtt aagaataaat ctccctcaac ttcaatgtct
     7921 ctatttatgt aggcttctcc aagtgctaaa gatgtacttt ttaatagttc agacttgcta
     7981 atatcattat ttactataac tttgaactga ggggagccct ctccaattag aaattcttcc
     8041 tcatctttaa gcctaactag aaatggtgtt tcaccaaatt tctctaaata gtcttttaaa
     8101 aaattttttg taatattcat attaccagct ccttctaatg taaatccagt aagtttccct
     8161 gcttaaaata tattatagtc ctttttttaa aaaagtaaaa ttttttcaat aaatttttta
     8221 tttaaaggaa atttattttt tataaagaaa tttcatgttg tagtatattt tttgcttata
     8281 aattttggta ctggatataa tattaaactg ttaatgatat aataaattta atctataaag
     8341 taaataaaat tttgtaataa atagttaaag ctattttata aggagatata tgatggaagt
     8401 atatgctttg gttgggtcaa gtggcacagg gaagagcttt aaggctttag agttcgcata
     8461 tgaaaatgat atagaatata taattgatga tgggatttta atatacaaaa acaaggtttt
     8521 agctgggata tcggcaaaac aagcaaatac tactatagaa gcagttaaaa gggctatttt
     8581 ttataatctg gagcatagac aagaagttag agaagcaatt aaaaaagaaa atataagaaa
     8641 aattcttatt attgggacat caaaaaaaat ggttaatcaa attgtagaga gattgtcaat
     8701 tggaagggtt tgcaagttta taaatataca agatataagt actaaatatg aaatagaaat
     8761 agcaaaacaa gctagaaaag aaggaaatca tgtaattcct gttccagcag ttgagataaa
     8821 aagtatggca agtggtctta gtataaattc tttgaagaga ctttttagaa aaggaagtaa
     8881 taggaatatg gtagttttag aaaaaacaat tataagacca acttttagtt atataggaaa
     8941 gttttatatt agtgctgatg taattaaaca gattattgaa tatgaagtat acaactttgg
     9001 aagcattgat agaataaata aaatcaatat agagaattgc aataatttta tgaatatttt
     9061 tgtaagtata aatatcaatg atttagaaac tataaaaaaa attgaagaca tacaacagac
     9121 aataaaaaag tctattgaga aaatgacttt agttaatgta caaaaaatag atattaacat
     9181 tcataaaatt aaaaaaataa acttcctcca atgttaaaaa ataaactgag aaaatgatat
     9241 actaatttgc aataaagtgt gctataatta aactgtaaat ttaggaaatg gttttttcta
     9301 aatggagtat acataattat aacgtattta taagggggct ttcaaatgtc aggaaaagat
     9361 gtaaatgtct tcgagatggc gcaatctcaa gtaaaaaatg catgtgataa attaggtatg
     9421 gaaccagcag tttatgaatt attaaaagaa cctatgagag ttatagaggt ttcaattcca
     9481 gtaaaaatgg atgatggttc tataaaaact tttaaaggat ttagatcaca acataatgat
     9541 gcagtagggc caacaaaagg tggaataaga ttccatcaaa atgtttcaag agacgaagta
     9601 aaagctttat ctatatggat gactttcaag tgttctgtaa caggtatacc atatggtgga
     9661 ggtaaaggtg gaataatagt agatccatca actttatctc aaggtgaatt agaaagatta
     9721 agtagaggat acatagatgg aatatataaa ttaataggtg agaaagttga cgttcctgca
     9781 ccagatgtaa atactaatgg tcaaataatg tcttggatgg ttgatgagta caataaatta
     9841 actggacaaa gttctatagg tgttataact ggtaagccag ttgaatttgg tggttcttta
     9901 ggaagaacag ctgctactgg atttggtgtt gctgttactg caagagaagc tgctgctaaa
     9961 ttaggaatag atatgaaaaa agctaaaata gctgttcaag gtataggaaa cgttggttct
    10021 tatacagttc ttaactgtga aaaacttggt ggtactgttg tagctatggc tgaatggtgt
    10081 aaatcagaag gttcttatgc tatatacaat gaaaatggtt tagatggtca agctatgtta
    10141 gattatatga aagaacatgg taacttatta aacttcccag gagctaagag aatatcttta
    10201 gaagagttct gggcttcaga tgttgatata gtaataccag ctgcattaga aaactctata
    10261 actaaagaag ttgctgaatc tataaaagct aaattagttt gtgaggctgc taatggacca
    10321 actactccag aggctgatga agtatttgct gaaagaggaa tagttcttac tccagatata
    10381 ttaactaatg ctggtggagt tacagtttct tacttcgagt gggtacaaaa cttatatgga
    10441 tactactggt cagaagaaga agtagagcaa aaagaagaaa tagctatggt taaagcattt
    10501 gaatctattt ggaaaatcaa agaagagtac aatgttacaa tgagagaagc tgcttacatg
    10561 cattcaatta agaaagttgc tgaagctatg aaattaagag gatggtacta agatttactc
    10621 ttaataaata ataggatata attatttaag atataaaggg gctgtacata gtacagccct
    10681 taaattatgt ggcttacaat gaagtaagct atttttgttt taacttataa aaaacttgat
    10741 aaattatgta atttagaatg tattttttac caccacatga ttacaaatta gaagagttag
    10801 attagatttt tagacatgaa cttatgcatt ttaaaagcaa agaccttaat ttaaagtatc
    10861 tagtattatt tttaaaaata gtatattggt ttaatccatt tatatatata atggaaaaat
    10921 atatggattt ggattgtgaa ctttattgtg atgagagagt tttaaaaaat tgtactgtac
    10981 aacaaaagca agaatatgct ctaactattg taaattcaat gaaaagaagt acaaattttt
    11041 caaataaatt tgtagctgga ttaaataaac aatctgatat tagaaaaaga gtaataaaca
    11101 tgttcagtga aaaacataaa aatggtatct taagggcttt gattataagc ttgttttctt
    11161 ctattacatt ttttaaattc gattcaacta gtattattga agagaatgat ttcttagaga
    11221 ggtcaagata tacgtttgaa ggaaggcaag aagacatgtt tgtaacaata gattttatat
    11281 atgcagatgt acctataaaa cttagaaaag agcatgaaga aaactgtaga gtcaatggaa
    11341 tagtaccaaa agattcagat tcaatagaaa ttattcctag attttataaa gattgtatgg
    11401 atttaaaaaa ataagttaga cttaatattg atgttgatat tatgaatttt ttatatttaa
    11461 gcaattgaaa attttattat agattcaatt ttatatgtgt ttaagtatga aatttataga
    11521 tagttgaaat aattaaatat gattgatgca taggaatcat tactaagcta taattaatta
    11581 taagggagtt taaataaatt atgagttaca caattataaa atgataaaaa
//
LOCUS       DAGSHT010000106        11506 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.108, whole genome shotgun sequence.
ACCESSION   DAGSHT010000106 DAGSHT010000000
VERSION     DAGSHT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.108"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(215..784)
                     /locus_tag="MJB72_002419"
     CDS             complement(215..784)
                     /locus_tag="MJB72_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBY8263507.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            1187..1759
                     /locus_tag="MJB72_002420"
     CDS             1187..1759
                     /locus_tag="MJB72_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBY8263508.1"
                     /translation="MEYIINPCSGIKIKITRGQKIKIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINFFMYTKVNTDGSIEVCEPLSKASDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(2438..3340)
                     /locus_tag="MJB72_002421"
     CDS             complement(2438..3340)
                     /locus_tag="MJB72_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBY8263509.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(3467..3586)
                     /locus_tag="MJB72_002422"
                     /pseudo
     CDS             complement(3467..3586)
                     /locus_tag="MJB72_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4133..4795)
                     /locus_tag="MJB72_002423"
                     /pseudo
     CDS             complement(4133..4795)
                     /locus_tag="MJB72_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(4955..5491)
                     /locus_tag="MJB72_002424"
     CDS             complement(4955..5491)
                     /locus_tag="MJB72_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBY8263510.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(5564..5977)
                     /locus_tag="MJB72_002425"
     CDS             complement(5564..5977)
                     /locus_tag="MJB72_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY8263511.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            6470..7108
                     /locus_tag="MJB72_002426"
     CDS             6470..7108
                     /locus_tag="MJB72_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBY8263512.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQGSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(7716..7895)
                     /locus_tag="MJB72_002427"
     CDS             complement(7716..7895)
                     /locus_tag="MJB72_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8263513.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(8121..8654)
                     /locus_tag="MJB72_002428"
     CDS             complement(8121..8654)
                     /locus_tag="MJB72_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBY8263514.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            8971..9321
                     /locus_tag="MJB72_002429"
     CDS             8971..9321
                     /locus_tag="MJB72_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263515.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYNW
                     RNKVFSILQRLIRLEKSKNELYMYEALVSVSFLWLIMIKNIVLPPIKHKNIINIHMQK
                     ILRYIEKYYCFTLY"
     gene            complement(10197..10769)
                     /locus_tag="MJB72_002430"
     CDS             complement(10197..10769)
                     /locus_tag="MJB72_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY8263516.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(10831..11094)
                     /locus_tag="MJB72_002431"
     CDS             complement(10831..11094)
                     /locus_tag="MJB72_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBY8263517.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(11094..11315)
                     /locus_tag="MJB72_002432"
     CDS             complement(11094..11315)
                     /locus_tag="MJB72_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBY8263518.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         4003 a         2018 c         1372 g         4113 t
ORIGIN      
        1 tattttattt accttagtaa aaatgtgtgt gtctcaaaat aaacttttta gttgatgagg
       61 cactcttttt tacatgaaat caaatatatt tttatccttt caacaatgaa aaagggattt
      121 atctctccat tttgaaacaa gccctttttc tattaattac tttcttaaaa aatataatac
      181 gatatataag ataaatatct aattgtacct atttctaatt atatctatta gaatacaaca
      241 cctttgcctt ttcagtaaac tccctaacaa aatctagttt actatctgta taagcatcac
      301 gattatgctc atacttcttc caaaggtcca acttaagttt ttcatactcc tttgcaacat
      361 ctgggtggtc aattaagtaa tctctaaaaa acaactcatc attatcacct acatacctta
      421 tatgcaaatg aaaaactcgt tcttcaaacc catctactgt atacccttta ttaagtgaaa
      481 gcctactact gctctcagac atacaaatat atccattcga ctcaatcaaa tctttaatat
      541 ctttcatatt ataatcagga attatctcaa tcagtatgtc aacaatgggc ttggcataaa
      601 tcgtactcac agaagtactt ccaatatgac ttattctaac aattttttgc attggcaata
      661 ttccagttag tctatattgt tcttctttaa accaattttt ccaacattct ttgtgagtag
      721 tcaaaaatat aggaaacaag ttccataact cctctaaagt catatcttcc aacttctttt
      781 tcattagtta cctcccttta aatttgtact ttatattttt tctttttcaa atttacttag
      841 ttaatattta actttatttt ttatcaaatc atttttctaa ttatgtaaat ttttctaaat
      901 ttagttttta atgtattata acagattttt acccttctta cacttttgta tctatttaaa
      961 tattagaaca ttttttctta cttctaaata cagtaagatt gcttaaatac caaataggag
     1021 caaaaaaact ctgtaatctt aaaaaaacta caacatcttt atgagaatta aaaaatgata
     1081 gactatgtta tcaacattaa gaaacagata gaaaagaaac agagtatgtg ctaaaatggg
     1141 aacatagaac agaatgacaa attggaattt atggaggaca ttaaatatgg agtatataat
     1201 caatccttgt agtggtataa aaattaaaat aactagaggg cagaaaatta aaattgtgga
     1261 tattgaagga ggacaggtcg ttgatttttt tgcagaaatg aaagatgatt caactgaatt
     1321 tatatctcca ggtgttacaa ttgattgtaa tgagtcatta agattacaag ttgggaattt
     1381 aatatattct aataagtata gacctatgtt taaaatttta tatgatgatg taagggaaca
     1441 tgacttgcta catccatgtt gtagacctga gatgtacgat ttcttttatc acaatggaga
     1501 aaatcatcca aactgttttg ataatattaa tcgcacactt ggaacttccc atcaaattat
     1561 tcaccccatt aattttttta tgtacacaaa agtcaataca gatgggagta ttgaagtatg
     1621 tgaaccttta tcaaaagcca gtgacaaaat tattcttgaa gcattggaaa acatgacttt
     1681 agggattgct gcatgcagtg tttcagaaag ttcttgtaat agcagaaaat gtacttctat
     1741 taagattttg gttgattgac aaatttcatt tatcgaaaaa atatcaaaat taaaaaatga
     1801 cgtcaaaggc gcaaaagggt aaccacttgc gctctcaatt cattatggtt tgttacgtat
     1861 ttatgggtaa aaatcccttg attctatgat gtttttcaga cacaattaca aatgagtaag
     1921 gattttatac tcatactcta aaaaatagca ttttattgcg taacagaacg aaaaaacagt
     1981 gtgtaccttt ttcttgcaat acacactgta ttcatagcca ctctagctgt taactgttaa
     2041 ttatctcata atacccttac tggtattcat ctaatcttaa ccccttttaa ttattttcat
     2101 ttataaataa attctattct tcccaaaata tcgtaattaa aatatattaa ttaaaatatc
     2161 ccaataagaa tatctgaaaa atcttctacc tattttttca aaccttttat tcccattcct
     2221 catacaatag tgcaactttt agcacagtat aaatatacca ttagtccata taatctcatt
     2281 aacaaatata tacaattatt ttcttcaaac tcttttatat ctaagatatc acattttcat
     2341 tttcttatat taattcctta actctctttc aaattaccaa ttttttaatc aaatcttcat
     2401 taaatattct atcattatta aacattcatc attatcccta aaaaatatgt gccgtatgtt
     2461 ctttacacaa attttccatc ttatcataca cagcaccact atctctctca tctgtcatag
     2521 tcacaattac atcacttggt ctaagaattg tcttacctct aggaataatt tcttctccac
     2581 ctctttgaat tgctaccaaa agacaatttt caggccactg tacatcctga attgccttgt
     2641 tcgcaacagg agagccaaac ataacagaga actggtctag tatcttttcg cccctctctt
     2701 tatgtacagg ttctccattc ttttcaagta acctctcaag aagactttca taaataggtt
     2761 tagacttcat aagtgtagcc acaatatatg ccacaattga aattactgat agtgaaagca
     2821 tttgactaac agaacctgtc atttcaaaaa ttaatatgat accagtcaaa ggggcacgca
     2881 caatagctgt aaaatatcct gccatagcca aaagcacaaa gttatttaca taatcgggat
     2941 tcattccaaa aactttaaca cctaccatac caaatattcc acctataaat gctcctaaaa
     3001 caagcaatgg aaaaaatatt ccccctggtg cacctgaccc aaaactaaca gccgaaaata
     3061 taaaacgtat aacaaaaatc aaaacaacca ttccaaaaat atatttatga cttgtaagtt
     3121 cttcaactaa attgtgtcca ctccccagaa catcaggcat aacaagccca agaataccag
     3181 ccattataaa tggaatcaac agttttccaa acgtaccaat tttaggaatc ttcttgtata
     3241 aagactgcac atatagagta aaccagttgt aaaatgcccc cataactcca agaataacac
     3301 ctaaaatcac tattaatcca taataatctt gtggcaacac atttccaatc tgaacctccc
     3361 atgactaaag tcacagggtt cctgcttcat agaattttgc atactaaaat atgtcttact
     3421 aattctccac aggctatccc cgtagttcct acggttctta cataagttat ttattttgaa
     3481 ttgttattag tcttaatcct tcttttaata tatttatact tgcatttata tctctatcgt
     3541 gagtttcatt acaactagga caaatccatt ctcttataat aatgttaaca aatgtaatta
     3601 tttaaaacaa ttatttagaa ggttttatat ttaattttaa ataacatatt taagaaccgt
     3661 aagtcttaac ttttatcatt tttgaggttg tcgttcacat aagttcgcta ctacctatgc
     3721 agttctctta tgaacttctt acactttcat gtaagcacag actatatctt atccttcggc
     3781 attatccgct aaggtctacc cacttccact accaatagct tgtagtgtac ttccctcaag
     3841 aggaatagtc gttgaagttt ctcctattcg gagcttacct gctgattgcc catttttaaa
     3901 gtacttaggg tttaacctta taccatctaa ccaatttttt ctactttcgt aactttcaca
     3961 cttaggttta tttcatcctt atgttttagt ttggttagct ttaggggttt ccagcaattc
     4021 aagtagtatt ggataggttc tatcctatct ctacatatag gtttccctat atgctgacta
     4081 ttttagctac taactcatga ctaaagtcac aagtgttcgt agctattttt taatcaaact
     4141 ggaatacaga ctccattcct aggaaattgg ctgaaatata gtctgctgtc aaagatgctg
     4201 tcatcacaga aattaaaaca gatactgaaa agtttttatg gacttcttca agtgaaaaca
     4261 taacacccgc tagtggtgca tgaaaggctg ctgataatcc agcacttgcc ccacaggtta
     4321 acaggaattt ttcctctgtc ttccctcggt caagagctct agaaatacct ttacctgcca
     4381 tagcaccaag ctgaatggaa ggaccttccc ttcccaaagc aagaccaccc atgagtgata
     4441 agaatcctcc aataaatttt gttggcaaca ctcgccacca tacttgtttt attttccctg
     4501 tcatttcccc ctcaagctga gggataccac ttccagaaat cattggctcc cagtccacca
     4561 actttgatac aatccacgca agtactgcaa gtactgcaaa ccaagctatt atttttattg
     4621 tagaaccttc tacaaatttt aaaatttggc taagccaagt accagcctgc cctaaaagta
     4681 tcctgtataa aagcacaatc aaaccagcca cagccccaac cataagtcct tcaccaatca
     4741 aaattacctg aaagtgctct gcccgtttga taatatgaga tgtatccttt ttcatactgt
     4801 tcctccttca tccaataatt ctacacaatc tgaatctcca atgactaaag tcatgagtgt
     4861 gtacagatat tttttaatca acaaaaatag atttttattt actaaatata agaaaataat
     4921 tgctcaattt ctcaataaat gtgaatttat tcttttaggt atttattctc cacagtcgtg
     4981 cttcttccag caaaatattt attagttctt gctttctctg tgtatagatt ttacgattgt
     5041 cctcaaactg taatgctaat ttttgtttat gactatcata gagttttgct ttgtctgtaa
     5101 aagtatttag ataatctcga aaattgatat aattattcca tgcaacacta ttccattcaa
     5161 caacatgaat atggtgagta cgagtatttt tctcaaaatc tcccatagca aaaagtaatt
     5221 gttttggtac atcctgacct cgaaaaacga ttccacattg ctctaacctt tcgaggtata
     5281 atttagcttc ctctaaagaa gcaacaccta caacaatatc aataataggc tttgcgctaa
     5341 tactgggaat agaagtgctt ccaatgtgtt gaatatcaat ggcagtctta tcccatatac
     5401 ttttcaataa tataatgaca tcttttgcac ttttatccca tatcatctga tgttccaata
     5461 atttaaccat accacatttt aaacctatca ctatatacca cctcaatata atccaccttt
     5521 atatatatta caattttttc tatcttgata aacagtgatt tagctatttc actttttctt
     5581 gacactcgct acattcccta tcatgaatag atataattcc tccacattta gaacaagtat
     5641 atctctcctt ctgcttttgc ataaacttct ctaaaccgta ctgctgaaca aatttactat
     5701 tttccataag gcttgcttga tatctcttgt tataactttt ttcaagattt tttatgagtt
     5761 tacaaggaaa attgaaacat tcaaaacaat aaaacaaccc tttttgacag acacaatcct
     5821 ttattttaca cttacggcag tgttctggct ttcctgtatc gccattcaaa caaccagcac
     5881 atggcttttt gtgataacaa tgcttatagc aaaccataca attcattcca caaggagcaa
     5941 acataatcgt atcaatactc tcttttggca ttttcataac attttactcc tttcaaaaat
     6001 ccaatttgtc gaattgttat atattcatat tgtaacataa aaaatagcta aaaaattcat
     6061 gcactttcat tgccaataaa ttttcaagga aatatgaaag tttaaaacta agaataaaaa
     6121 gagataaaaa ggaagctact aatcaaaata ttcaaataca agtaatcaat ataaataact
     6181 taaggtatat ttaaaagtat atgatattac cagcagtttt aaatacaaat ataaccacac
     6241 atgcctcaaa aacacttatt acagtatctt agaaacttag acaatacata agattcatta
     6301 ataaaaaagt tctcaatata gtcattaata aaaacgttct caatatagat atatttaacc
     6361 atgttttgaa tagcagataa ttatatgatt aaaaattaaa tatgacatag cgtttgtgga
     6421 atataacacc taaattatgt tataataata aaacaggagt gtgatagtga tggatgaaaa
     6481 aatattagag ttattacagg aaatgcaagg taatattagt aatatgcaag gtagtatcag
     6541 tggtatcgaa ggcactatta gcgatatgca aagcaatatc aatggtatcg aaggcactat
     6601 tagcgatatg caaagcaata tcaatggtat cgaaggcact attagtagta tgcaaggcaa
     6661 tatcaatggt atcgaaaaca ctattagtgg catgcaaggc actattggcg atatgcaagg
     6721 tagtatcagt ggtatgaatg gcactattag cgatatgcaa ggcagtatca gtagtatgga
     6781 tggcactatt aatagtatgc aaggcagtat tactgatatg caagttgaaa ttaaagaagt
     6841 caatagtagg ttagacaata tggaacctga tattaaagac ttaaaaactg gacaagttga
     6901 aactagtagt agacttgata ctattcagag tgatgttaca gaaattaagt cagatgtaaa
     6961 aattaacaga aatacattag atacagtgat taaacatacc tcttcattag taaaagatac
     7021 atcagatatt aaaaatgatt taaataatat agagatagtc acatctaaga attcgaatga
     7081 tattgctaaa ttaaaatcag ttaaataata tactatagta aaaaatacct gaaaatttca
     7141 catacttctt taaagttata ccaaatttta tacagaaata ctgattatcc gacaacacac
     7201 tttacttata aataatttat taaaaaaata ctcattttat cctaaaatta acacgactaa
     7261 atgagtcttg atataataat aaaaaataag ggagattgga cactaaaaag ttgtgtatct
     7321 cccttatatt ctataatata gttatataat ttttcagcct aaaataaaag tccatctttg
     7381 attaaaacac atccccttat cacttataaa aggataaaat cttgttttca tttttccatt
     7441 tatatcttct tgcttaagag taataatttt ttcttttgat actttaagtg tttggttaaa
     7501 tttaaccaat cgactaagtt ttgttcaata tctatttttc atattttcca atacttaatc
     7561 tattttttat aaataattct ttcatttcta actcacttac attttttaca taaaacgaaa
     7621 catataaatt tcaaccttat cttgtatggt tcttaatact taatgagtag cttaagtatc
     7681 aatcattata aatttttccc ttacatttaa cactcttacc atttccaaaa tatacaaaat
     7741 atattttgaa catttgtaca atgatgaaat acaatacatg aaaatacaat atcataactc
     7801 ttgtctaaaa atagaagttc ttcaacataa cctttcacaa aaaacatatt atctaagtat
     7861 tttttgtctg ctccttgttt tcctgtttgt agcatataaa ttactgcatc taaacatata
     7921 actactcaca cgaaaaaaga aaataaactt ccaaaataca agctctaaaa actacctcta
     7981 aagttgtata atgtttacta ggaataaggt tgtataacat atagtttaaa taatcatttt
     8041 ttaaaattgc aagttattgc tttcaaaatt agatgttttt atatcaaata aatgattaag
     8101 aatttctaaa tttaatggct ttataatgca ctccctttct ttgggataaa ataatgttgc
     8161 acagatactt tttctaaaga taacaatttt atttttctat caattccacc agcataccca
     8221 gtcaaactcc catttgttcc tacaacacga tgacatggaa caataataga gattccatta
     8281 tgccctactg caccaccgat tgcttgtgct gacattttag atattccttt ttgttgagca
     8341 actttatatg caatttctcc ataagtgatt gttttaccat atggtatttg ttgtaaaatt
     8401 ttccacactg tagtctgaaa aggagtacct accatatgga gtgatggtgt aaaattgggt
     8461 tcttttcccg aaaaataaat atctaaccat ttctttgtct gctcgaaaat tggcaattct
     8521 ttttcttgat gttcttggtc aagattataa gcataatact tttgattctc aaaccaaaga
     8581 ccagttaacc caatattatc tgatgctaaa agaatatttc caattggact atggtaatag
     8641 ctaatatatt gcatacaatt cctccttata ttctgaaaac ttcactcaaa tcattagaca
     8701 cttcttttct atctaccatt atgtaaaaac atactaatat aatttccttc taactacact
     8761 ttcgttataa tatctatcct catatcatat tagttttata tttacctgca tctgttcatc
     8821 cttttttccc tttccctttg atgaatataa aattcataca tttaagcttc ctactgtatt
     8881 aacttttata gaagtgtata ttataatttt atcaccaaat agaccaaaaa aatatcgata
     8941 taacatcaag taatatcata atactttcaa atgggaaaag taagaatagg ctgtaaaaaa
     9001 gaaaagtatt tttatgttag aaatccagta aaatcgttag tagatagaat aacagaaaac
     9061 gaacaatttg ctatttatca ttttacacat aaatataatt ggcgcaataa agtgttttct
     9121 attctacaac gattaataag gttagaaaaa agtaaaaatg aactgtatat gtatgaagca
     9181 ttagtttctg tttctttcct ttggcttatt atgataaaaa atattgtttt gccaccaata
     9241 aaacacaaaa atataattaa tatccatatg caaaaaattt tacgttatat tgaaaaatac
     9301 tattgtttta cattatattg aagggtacta ttgtgaaaac ttaattttgg agtactatca
     9361 agcagtggta atatcaataa atcagagtgc tccagatgat ttgaactaag tttaaatact
     9421 ataccttata aatattttac tgagttttgt ttattaaaag caactcaatt tctaaaaggc
     9481 acaaatgagc taataagtga cattgctata aaagttgttt ttcatcaaat aatctatttt
     9541 agtaaatgtt ttaaagaaaa aacgggatat tctccaaaag tgtacagaaa tataaaaaaa
     9601 gagatatgat tttttatttc ttttgattat ccaaagagta aataaaaaat attgtaaaaa
     9661 acttactaac aacggcaatg ctttgaacta tggtaatact ttgaactttt gattggactt
     9721 tttttgctaa aataatatat ttaaatagca aagtaatctg tttaatcaat aaaaaataag
     9781 agataaaaga aatcaactaa aacttttacc tcttattttg atagtttatc agagctaata
     9841 tttacactct ttttattttt gatacttcat taaaaatgat gcttattctc ttttcatttt
     9901 taatacttca ttagaactta tgcttattct cttttcactt ttaattcttc attagaactt
     9961 atgcttactc tccattcact tttaattctt cattaggact atgcttattc tccattcact
    10021 tttaattctt cattaaaaat gatgcttatt ctccattcac ttttaattct tcattaaaaa
    10081 tgatgcttat tctccattca cttttgattc ttcattaaaa atgatgctta ttctctgttc
    10141 acttttaatt ctttattaaa actgatgctt attctccatt cactttcaat tcttcattaa
    10201 aactgatgct tgcactctgt aaactcataa acatccatta atgcttcacc aaatctttga
    10261 tagtgaacta cttccctctc acccaaaaat ctcaatatat ctttcaaatc agggtcatca
    10321 gttaaattta ttaattgata gtacgtagcc aatgcttttt gttcagcagc catatcctca
    10381 tgtaaatcag ttaccgaatc gccaaagaca ttaatataac ttgtagtaaa gtttactcca
    10441 ttagaatcta ctggctgaag accatgtcca aaattagcat aattactacc aagtccagca
    10501 gccttaagtt cttctggagt acaattgcct attaattgat ataacactga agaaattatc
    10561 tcaacatgag ccatttcctc tgtacctata tcagttaaaa gtgcacgaga ttttccagtt
    10621 ggcatagtat atctttgttg aagatatcta gttgcagctg ccaactcacc attaggtcct
    10681 cccaactgag tcatgatata ttttgccatt ctagggtctg cttgtcttat gttaactgga
    10741 tgttccagtg ttttttgata aatccacata ttatcttact cctccttaat atatatttaa
    10801 taataaatct aattttaaag tttaataatt ttatttataa aatgatttgt cccatggcca
    10861 aggttcagat atccatgacc aaggatattc acttgattca tatccaaaat tagttaatgc
    10921 tccatattta gattcataat cacacacagc ttgtgtaaat ttatcgcagt atctgttgta
    10981 tgaatcaaga gctttcttat cccttggatt gttatctaaa tataaattta attctataca
    11041 ggcaaattga tattctgaaa ttctctctaa aagttctctt ctatttaatg acattagtac
    11101 atgttacccc cttttttagc tctaactcta tttttaggat ttgcatatag ccaatcatta
    11161 tagttatttg actctattaa atctaattca ggaaataaag tacctctttt taatgctgtt
    11221 tttggattgt atgtgtcatc ataaatttga cgattgatgt atggtctagc aaattcacaa
    11281 caatagtctt gtttatcctc ttttaaatat cccattaatt atcctcctta atttttagaa
    11341 ttttaaaata gcattatttt taaaaattgt atactggttc tatattataa ctggtagttt
    11401 taataggtct ttaataagat taaatgatta aataagtatc tttttttctt attttacctc
    11461 taaaaatttt tctaattagt ctttcataaa tatctatttt aaataa
//
LOCUS       DAGSHT010000107        11472 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGSHT010000107 DAGSHT010000000
VERSION     DAGSHT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.109"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     regulatory      <1..74
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            236..757
                     /locus_tag="MJB72_002433"
     CDS             236..757
                     /locus_tag="MJB72_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY8263519.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            908..2584
                     /locus_tag="MJB72_002434"
     CDS             908..2584
                     /locus_tag="MJB72_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBY8263520.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            2596..3804
                     /locus_tag="MJB72_002435"
     CDS             2596..3804
                     /locus_tag="MJB72_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBY8263521.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSIKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            3819..5531
                     /locus_tag="MJB72_002436"
     CDS             3819..5531
                     /locus_tag="MJB72_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBY8263522.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            5524..6432
                     /gene="pilO"
                     /locus_tag="MJB72_002437"
     CDS             5524..6432
                     /gene="pilO"
                     /locus_tag="MJB72_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBY8263523.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            6459..7025
                     /locus_tag="MJB72_002438"
     CDS             6459..7025
                     /locus_tag="MJB72_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY8263524.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            7062..7589
                     /locus_tag="MJB72_002439"
     CDS             7062..7589
                     /locus_tag="MJB72_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY8263525.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            7610..9148
                     /locus_tag="MJB72_002440"
     CDS             7610..9148
                     /locus_tag="MJB72_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263526.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9244..10326
                     /locus_tag="MJB72_002441"
     CDS             9244..10326
                     /locus_tag="MJB72_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBY8263527.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10307..11089
                     /locus_tag="MJB72_002442"
     CDS             10307..11089
                     /locus_tag="MJB72_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBY8263528.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKKLLELYFSMVI"
BASE COUNT         4725 a         1201 c         2053 g         3493 t
ORIGIN      
        1 aacgttgatt tatgttctgt aatgtgggca ccttggagca tattgagtta gtggtgcaac
       61 cggctatgaa attgtattta tttataaata ctattatttt catagctttt tttatttaaa
      121 aaatggttat atgttagaaa taaatttggt tagtacgttt aaataatttt acttagattt
      181 ttaaccagat atattgtaag taagtattaa ttaaaaaatt taagggggaa taaaaatgaa
      241 gttaaaaaag aataaaaaag gtttcacttt agtggaatta ttggtagtaa ttgcaattat
      301 aggtatatta gcagtagtgg cagttccagc tttatttagt aatataaaca aggctaaggt
      361 agcaagtgtt gagtctgatt atagttcagt taagagtgca gcattatctt attattcaga
      421 tactaataag ataccagtta caccagatgg tcaaactggt ttaagtgttt tagagactta
      481 tatggagtct ctgcctgata aagctgatat aggtggaaaa tataaattga ttaaagttgg
      541 taataaattg gtattacaga taggtacaaa tactgaagga gttacattaa cagaggcaca
      601 atcagcaaag ttattgagtg atataggtga aaataaaata tatacaagcg ctacaacaaa
      661 tagtttggga aatccattaa caagtaatac aaaaatagat aataacgttc tatatatagt
      721 acttatagat aatactgtga tggacacaac aaaatagtta aaacaatatt tttataaaaa
      781 tattgtaaat ttaatgaaaa atataatata aaagacagga agaaaatctt cctgtttttt
      841 tatactattt tctttaatgt tcaattatga atgatgatat aattgagata gtaaaggagt
      901 gaaacctgtg gctaaaaaag ttagaatagg agacaaactg gtcgaaaaag gatatataac
      961 agaagaacag cttaaatggg cactatcaga acaaaaaaac agtggaaaaa gactaggaga
     1021 atttctagta caagaagggc ttatagatag taacctacta ataagtgtat taaaagaact
     1081 cttggatata gaaagtatat ttttagaagg gacagaaata gacactttag ctacaaagat
     1141 ggttccagaa aatatatgca aaagatatac tgtatttcca ttcaaaatag atggaaataa
     1201 aatatgtcta gccatgtcag acccacaaga cagagaagca gttcaagatg taaggcgtat
     1261 gtcaggtaaa gacgtagaga tatttatatc aagtacagaa gatataaaca aagcaatagg
     1321 tcatgcttat gcacattcag aaataaataa ggcaatgact gagtacaaca aaaatagaac
     1381 tggtggagta agagaaacag taatacttga agaagatgta aatgctgctc caatagtacg
     1441 attagtaaat aacatactag aaaatgctgt tagaatggaa gcaagtgata tacatataga
     1501 acaaagtgaa aattacatgc gtgttaggtt taggatagat ggaatgttaa gagaatatat
     1561 gaggatgaac tcagcaccat ataaggcagt aatcagccgt atcaagataa tgtcagatat
     1621 aaatatatct gaaaagagaa taccacagga tggtagaatc tatttaaaag tggataataa
     1681 acccatagat tttagggttt ctacaatgcc aactaacagg gatgaaaaga tagctatgag
     1741 ggttcttgat aagagtaatt ttatggttag taaagaagta ctaggaatag acgaacatgg
     1801 ctctaagata tatgatgaat tgataaacac tccttatggt cttatattgg ttgttggccc
     1861 tactggaagt gggaaaacca ctacattgta ttcaatgtta aatcaactaa atacagaaaa
     1921 tagaaatcta ttaacaatag aagaccctat agagtatgaa ttacctggtg taaatcaatc
     1981 tcaaataaat gaaaaggcag ggcttacttt cgccagcgga cttagagctt ttatgcgtca
     2041 agacccagat ataataatgg ttggagagat aagagatact gagacagcag aaatagcaat
     2101 tagagcttct cttacaggac atttagtttt aagtacatta catgcaaata cagcagttgg
     2161 agcaatttca cgtcttttag atatggatgt ggaaagtttt ttaataactt catctgtttt
     2221 aggtgtaata tctcaaagac ttacacgaaa aatatgtgag cattgtaagg tttcatatga
     2281 agcagatatt ggagaaaaaa aggctcttgg tattgatgta aatgaatcag taactattta
     2341 tagaggaaaa ggttgtgaaa ggtgtaataa tactggatat aaaggacgtc taggtatatt
     2401 tgaaatgctt gaaattactc cagaaataaa agagttaata gattcatctg caaatcaaag
     2461 agaaatttta aagatggcta gaaaacaagg tatggttagc ttaaaagagg atatagttaa
     2521 aaaggtttta aatggaaaga caacagtaga agaaatgatt agaataattt tgatgactga
     2581 ttaaaggatg atattatgaa aagttttaaa tacacagtaa taaaagagga cggaaaaaag
     2641 caaactgatg ttattgaagc caacgatttt aatgaagcta gaaatcttct tagaaagaga
     2701 aatcttagag ttattgaaat caaagaaagc aaaaataaaa atataagatt atctagtata
     2761 aagagaaaaa aagacttagg agctgaccag ataagccact tttgtagaca atttgctatt
     2821 atagtatctt ctggtataaa tagtatttct ggattagaaa cattagctag aaggtcaact
     2881 aacattacat taagagaaga aattaatcgt atagtagcag aaataaaagt aggttctact
     2941 attgcagatt caatgctttc atcaaaatca aagtttccaa agttacttgg tgctatggta
     3001 gctacaggtg aagctacagg taaattagat gaagtattaa aaagtatggc ttcattttat
     3061 gcaagtgaac acagggttaa acaaaagctt agaaatgctg caacatatcc attaatagtt
     3121 ttgatttctt catttataat gatttttata tttacaacat ttatggtgcc taagatgatt
     3181 aattcaatta caacagtagg agcaactctt ccacttataa caagaattgt tatggggttt
     3241 ggtatgttta tgaagaagtt ttggataata gtacttttag ttattatatt atttatctat
     3301 cagtttaaaa aatatctaaa aacgtcagtt ggaagggctc ataaagatag agttataaat
     3361 aagatacctg ttttaggaaa aggaataaat tgtatggtag ctacacgatt ttcaagagca
     3421 ttgtatctat ttgtatcaac aggataccca ttagttcaag ggcttgatta tattatagat
     3481 agtgtaaata acactatggc agagaagatt ttagcatcag ctaaagatgg aataacaaga
     3541 ggagaaggct tagcagaaaa tttagagaga tatacgtatt ttgactctgt gttggtacag
     3601 atgatagcta taggggagca gacaggggaa ttagagaata taagtagaca gatggcagaa
     3661 ttttatgagt atgaatccga gatttattta aataggatgg cctcaatgat agagcctgtt
     3721 ttaataattg cagttggtat tatagttgcg atactggttg tatcaatatt catgccaatg
     3781 cttagtatat atgatgccat gtaggaggga aaggacaaat gaacaaggtt tatgtatcat
     3841 attatggcga ttctatttcc atagttgagg gcagatataa acacgataag gataagtttt
     3901 atgtaaaaaa ttttaatgtg ttttcaatag aggatattgc acaagatttt tctaaggaaa
     3961 agtatgctct attgagatac gcattagaaa atgttaagtt aaaatcaaga catgtagttt
     4021 tttgtttaaa tacaagagat gtaattttaa aaccccaaaa acttcctaat atggacaaaa
     4081 aggatttaga cggatttatg aagctagaga tggacgaaat gatggctttg gagtcagatg
     4141 aatatgtatt ttcgtatgaa gtatctagtg aaacacctga taattcagat ggcgatgaat
     4201 caagcttaaa tcttattatt ggagctatac aaaaggaaga ggtaaatgca attgtagaga
     4261 taatgcatga atttgattta gtattagata gaatagatac attatcaaca gcttacttaa
     4321 gaatgcttaa aaatttagac tacaatgata taatggtaac caatataagt gacaatagca
     4381 caattataaa tatatataag aaagatactt tatttattag tgataatata cctataaaga
     4441 taactgctaa agatatggac atgcaattat taaatgtagt agaagaatca aaaggtctta
     4501 tgaattacta ttcgtcaaga aactttggta aggttacgga tactatatta atattaggaa
     4561 aaagatattc caataaatta atttatgaaa cttttagctc tgcattttca agctatgttt
     4621 ctcaaggcct tactgagata atggatatat ctgatatgat aactggagat atagatgaag
     4681 atgatataaa ttcaattgta gagcttttag gaagtatgat agaacctaaa gggaaaagag
     4741 attttgaaaa tataaacttt ttaccaatgg atatacttag aaaacagata aaagctaaac
     4801 aaatgaaaaa catactgaaa attgctccaa tagttatttt gattcttgct ataccttatt
     4861 tatgtttaat tttttcaaat aatttagcaa ataaggaact tgaaaatgtt aatggtgaaa
     4921 taagaaatat tgaatctgca tattatcaga ttggaaatat agaaaaaatg atacagagta
     4981 aaagcgaaga aataaaggta tatgatatgt taattgggaa aaaggtaaag tgggaaaatt
     5041 tattggatga tatagataaa aatactccaa gtaaagttga actaacaagt ttatcaacaa
     5101 cttatcaaaa tcttggaact aataataaag ctgataattc aaaaagtaat aagactacaa
     5161 cagataaatc aagtaacaat gaaacaaaat caagtacgaa aagtgatagt aatggagata
     5221 aaacagataa tgatttaaat gaagataaga ctacaaatgt gtcagaagca ttaactggaa
     5281 atagtgagac taatagtaat aacacggata aagatgataa aactccattg tatgataaga
     5341 taccaaatgt tataaatata gaaggaaatg ctaaagattc ctcttatgta ggtcaatttt
     5401 tatataaatt aaagggatta tactatttca aggatgttaa attacatagc ataaaaagta
     5461 atgcagaaga ttctaactat acatttagta tgacattata cctgaaagaa ggtgtattaa
     5521 ctaatgaata aagatatatt taaaaaagat ataaaagata tatttaaaat ggaagttagt
     5581 ctaaaaggga ttcttataaa ggttataatt ttattagcta ttttatcata tggttatttt
     5641 ggactttacc ctcaatatca gaaatatatt gctacaaaaa attctgtaaa acaggctact
     5701 gaaaaattaa atatatataa agagaaaact gcacttatgc caaaatatga aaaacaactt
     5761 gaagaagttg aaagtgaatt aaaagaaaaa agcaaaaatc ttttatatga tatgcaagat
     5821 ggtctttttt taataggttt agataaaaaa atgaaggctt ataatattga cttaaccagt
     5881 tatgacgttg gaaatgttaa agaatacgaa catttttatg gtatatcaac aacaataagt
     5941 gttagaggtg attatagaaa tgtcaaaaaa ttaatggctt atttagaaga acaaaaaaat
     6001 gttactcaaa tactagatta tacaatgact tcatatatag aaccagaaaa agaagaggaa
     6061 gaaacaacaa acaataagga tttaattaaa aatgtgaaca taaataaaga tacaaaaata
     6121 tattgggtgg aaggcgaaaa taagtggcat ttatataagg attgtccatt gataaaagag
     6181 ggagagatta taaaaaatga caaactttta gataggtcta ctaattatct atgtaatact
     6241 tgtgtactta gagctgcaac agaaaaagaa gcaaaaaaag aagtcgtagc tccaaaggca
     6301 gaaggtgtag tagatgctac atttaaattt ataatgtaca ctaaagagaa tactacaatg
     6361 atacttgata cagataatcc atcttcatgg agaccaggaa aatataatcc atttaagtct
     6421 agtgcgaaat aaagctaagt aagctttaaa gataggtgat gagtttgtac aaaaataatg
     6481 aaaaaggtct tactttatta gaagtaataa tagctgtttt tatactgaca atagttttaa
     6541 gtatttctta taaagtgttc aatggtataa catcagcagt aaaaaagcaa cagattatta
     6601 cagatgctca agtaaatatt aatctaatta ataagtatct aaatagagat ttggaaaact
     6661 gtaaagagct aactaaaact ggttcaggta ataattatga atacaatata gagatgccag
     6721 ataatgttgt aaaatatgaa gtttctatag aaactaaaaa aaatacagag gtatattctg
     6781 taacgagaat ccaaaacaat acaattgata cagaaaatga agttagagaa gagataatct
     6841 ataatcaacc actagttcaa aacaataaag aaatgaagga aacaccattt aaaatagaaa
     6901 agcaaactgg taaatctatt tatactgtaa gtatatacta taatgaatca gtgcaagaaa
     6961 gtcataaaaa tagcaattta aataacaaaa cttatacatt tgatgttatg tcaagaatag
     7021 gataataaga atttattaaa aaataagtag gttgataaaa tatgagtaaa aagaatagtg
     7081 aaagaggatt ttccttaatt gaagtgttag tagctatggc tattatggga attgtactat
     7141 ttgcattttt taatattatc aatactaata acaaagcaaa cactaaaaat gacacagata
     7201 taacttcctt aaactatgtc caaagtgaaa tagaaaatct aagagaaaag ataaaaagtg
     7261 gagaatttga ttttgatagt ttagataaac tagaagatgg aactgtcgta tatgaaaaat
     7321 taatagataa atcaaaaaaa gttgtatatg ataaagttct tagtgaaggt gacgtgagct
     7381 tatatgatac tccatatgaa aagattacaa caataaaaga tgaagatggt aatttaatcg
     7441 acaaaggaaa tattacaaat aagataaaga ctattgtaga agataaaagt ggtcagatat
     7501 ataaaatagc tgtaactggg aaaagcatga atgactattc aagtaaaaaa gaggttaaaa
     7561 ttgtaactga aatatttaaa gataaataat tttacttggg ggtgagattt tgaggaagtg
     7621 gaataaattt aaaagtgaaa gaggggcagc tttagtttta gttttggttg tagtagcatt
     7681 gctttctatt gttggattga tattttcaaa tcagatagct aatagaataa aatctacaaa
     7741 gactactaat gaaggtatac aagcaaagta tttagcagag acatgtgtgg aaaatagtat
     7801 tgataaagca tatgaaaaat tgtatgatga gcttgagaaa atggataatg agtttaaaag
     7861 tgaaaatcaa gaaaaatcca tatctaggtc aaaattaaga aatatatcag atgaagattt
     7921 taataatcaa gatgaggaaa atattgaagc agaaagactt ggatatatga ataatataaa
     7981 tttctattta aataaagctt cttcagactt ggaaaaagct agtatggaat tgaaaaagct
     8041 atatgattta gatatgttgg actatagaga cattgaatat gtagatgcaa atattatttc
     8101 acatagagat tctatcttag aaatttgcaa gaattatact tctggagata ttagtaaaat
     8161 aaatgaatat attctgaaag aagatataga ctcaactaca ttgattgagg caaagttagt
     8221 gaataatgat attcttttaa aaaagttttt agaagaaaat aaaattgaaa atgaacattt
     8281 aaattcagcc ttttcacata cttataaggc attagataac atttctttgg cgatgcaaaa
     8341 tatgattgag tatagacata cgtttcatat agatgagcca aaagtagaag tatctaatgg
     8401 gattccagac tctcaacaat attatgaact aatacaaaat cctataataa atagtatgga
     8461 gtatagctgg aatagtaaat gggatacatt agaaaaccta ttggaaattt taccaaatca
     8521 aactcaagga tttaattctt taagagttca tttgagaaat aatgtaagaa aatttgagaa
     8581 gttaagcgat aatatatcta gtggtaaaaa aaatacagca aaaaatttct taaagtataa
     8641 agagttattg tatgaaatat cagaccaatg taatcaacta aaaagtatgt cttatgagaa
     8701 aatacctgtt aaatatgata acatggcttt gataacaact tttgactata tacaaaatga
     8761 gcttttagca gagataaaat gcagattaaa agaattaaaa ccacaagaaa ttgataaaac
     8821 agaaggtatt actataaaaa taccttttta taaggctgac tacgacatga caaaagaagg
     8881 atggcctaaa ttaaaagaaa atggttcagg ggcggagtta agcttaatgg taactggtga
     8941 taaaaatgga ataaaagaag tggaagttac agatggtaaa aaaaatatta ttggtttagg
     9001 tgttgaagaa aattcaaatt caaagtataa ggttgacgcc attgtaaact ttaatctaaa
     9061 tatagataca aatgtagttg gaaattatga tataaaagat aaaattttga ttaatcatga
     9121 tataagttca tacaaaaaag taaactaaat ataagaagca acttagtata aataacaaaa
     9181 taattttatt atttactact aaaaaaataa gtaaactaat agaagatatg gagagtaaat
     9241 attatgtata tttatgattt attagagcaa ggtataagac taaacgcatc agatatacac
     9301 ataacagttg gaacaaatcc tgtagctaga gtaaaaggtg gatttgtaaa actaagtgaa
     9361 caaatattga catcagaagt aacaatgcaa atggcaaaag acatagcagg ggaatctatg
     9421 tttaaagtaa tagaagaaca tggagaagct gatttttcag cttctctaaa aacaggagaa
     9481 agatttaggg taaatgcata taggcaaaaa ggaaattacg caatagcaat aaggactata
     9541 acagcagaaa ttccaacatt tgaaaaatta ggccttccag agagtataaa atcttttact
     9601 gaaaagcata aaggattagt tttagttaca ggtccaacag gaagtggtaa aagtacaaca
     9661 cttgcaagta tgataaatat aataaatgaa aaacaacaaa aacatataat tacacttgaa
     9721 gacccgatag aatatgtaca tcatcataaa caaagtttag taaatcaaag agaagtagga
     9781 actgatacag aaagttttca ttcagccctt agagctattt tgcgtcagga cccagatgta
     9841 atactaatag gagagatgag agaccctgaa actgtttcaa tagcattaac agcagcagaa
     9901 acaggtcatt tagtattttc aacactacat acagtagggg cagctaagac aattgataga
     9961 atagtagaca tgtttcaacc atcccaacag caacaaataa aaacacagtt atctacagtt
    10021 tgtgaaggtg tggtatcaca gcagttactt cctacagcag atgggaaagg cagaatagca
    10081 gcaattgaat tgatgtttgc tactccagca ataaaaaacc tgataagaga aggcaaaaca
    10141 tatcaaattc caaacatgat acaaactgga gttaaagtag gtatgaaaac tatggaccaa
    10201 gatttaatgg aactttataa aaatggaaag attacaaaag atatggcact tagtaggtgt
    10261 actgaccaag agtttatgac aagaatgata ggtggagtaa actacaatgg atattataat
    10321 cgttaatcta tatgtattta tagtgggtat agtgtttgga agtttcttta acgtctgtat
    10381 ttttaggatt ccagaaggta tatctatttc tagaccacct tctcattgta tgagttgtaa
    10441 tactagacta aagccaatag acttaatacc agtgctaagc tatttaatga gtggcagaaa
    10501 gtgtagatat tgttatgaga aaatttctag tagatatgct atagtggaat taattacagg
    10561 acttttattt ttaacagtat ttatgttata tggaatatct acatcaacta tttattattt
    10621 ggttcttgtg tctttactga ttataattac atttatagat attgaccact ttataatacc
    10681 agataaaata ttggtttttg gagctatatt ttctatgatt tttaaccttt tatttaaaga
    10741 aattccagtt aaggattcaa ttctaggtgt ttttatttgt ggaggaagtg tctggattat
    10801 agttcttttg attgaatttg taataaaaaa agagtgtatg ggtggaggag atattaaact
    10861 atttgcaatg ctaggccttt acatgggtgt taaaaatggt cttttgacag ccttactaag
    10921 cgtttatgta ggagcagtct atggaatctg tgtaataatt ataagtagaa taaaaggaaa
    10981 ggaatataat tctgttatac catatggacc ttttatatct attggagcat taataacaat
    11041 cttatgtgga aagaaattac ttgaacttta tttttctatg gttatataga tgatagtcaa
    11101 gttaaataaa gttttaattt aaaaaaatga attaaagtat ttaataagga caatattatt
    11161 attaacccta ttactaaagt gctacaaact atggagtata taataataat ttccttattt
    11221 tttgcacatt tgataaataa atctctatgt gttttttgtg atagagaaaa gaaaaattta
    11281 tacaatacgt tttttattta cataataact gtagttttat ctgtattaat atatctaaaa
    11341 tttgggatta gtgtagaatg tgtacaatat atggttttaa ttccattttt aataattata
    11401 tccattgtag attactacac aatatatgta tatgatataa caatagttag tggtataata
    11461 gttcaaggta ta
//
LOCUS       DAGSHT010000108        11387 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGSHT010000108 DAGSHT010000000
VERSION     DAGSHT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.110"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..126
                     /locus_tag="MJB72_002443"
     CDS             <1..126
                     /locus_tag="MJB72_002443"
                     /inference="COORDINATES: protein motif:HMM:NF013921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8263529.1"
                     /translation="PHLKSSMPSMWTNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            123..1286
                     /locus_tag="MJB72_002444"
     CDS             123..1286
                     /locus_tag="MJB72_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8263530.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            1646..2224
                     /locus_tag="MJB72_002445"
     CDS             1646..2224
                     /locus_tag="MJB72_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY8263531.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            2263..2841
                     /locus_tag="MJB72_002446"
     CDS             2263..2841
                     /locus_tag="MJB72_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY8263532.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            2927..3556
                     /locus_tag="MJB72_002447"
     CDS             2927..3556
                     /locus_tag="MJB72_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY8263533.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            3546..6305
                     /locus_tag="MJB72_002448"
     CDS             3546..6305
                     /locus_tag="MJB72_002448"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBY8263534.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            6392..7954
                     /locus_tag="MJB72_002449"
     CDS             6392..7954
                     /locus_tag="MJB72_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBY8263535.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            7955..9091
                     /locus_tag="MJB72_002450"
     CDS             7955..9091
                     /locus_tag="MJB72_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBY8263536.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            9155..10231
                     /locus_tag="MJB72_002451"
     CDS             9155..10231
                     /locus_tag="MJB72_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBY8263537.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            10209..>11387
                     /locus_tag="MJB72_002452"
     CDS             10209..>11387
                     /locus_tag="MJB72_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBY8263538.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     E"
BASE COUNT         4568 a         1172 c         2139 g         3508 t
ORIGIN      
        1 ccacatttaa agtctagtat gcctagcatg tggactaata gctattttgt aggtactgtt
       61 ggttctgtga gtttagaagt agttaaacaa tatatagaaa atcaacaagt taggacaaag
      121 aaatgataaa aactcataga gtaaaattaa acctaactag aacacaattt gaattagtaa
      181 gggaaaagca aatggaaagt gctaactgtt ggaactacat agttaactta tctaaagaat
      241 actattttga acataaacaa tggattaaaa agaatgatat acaaaagtta atcaaaggta
      301 agtacaatct tcatagtcaa actattcaag ctatatcaga taaatttgat gcaaatagaa
      361 agactatatc agaattaaga aaaaaaggta atactaaagc aaaatatcca tacaagacta
      421 agaaatttta tataatacct tttaaagcta gtgctattaa tagaaatgct aaaggcaact
      481 taaaattatc aatgtcaaaa ggcaggtatt tagaattaga ttttaatgtt gagaatataa
      541 aaactgctga aatagtatgg cgaaatggat attatttata ctacactttt gacaatgaat
      601 taaatggagt tgtagccaaa ggcactaata ccgtaggtgt agacttaggg gaaatacatt
      661 cgatagctag tgtaacaaat aaaggtgtag ggcttattct atctaataga gaaggcagaa
      721 gtattaaaca atttagaaat aagatgtatg cttatatatc taaaagatta agtaaatgta
      781 aaaaaggtag tagacaatca aagaagttat ggagattaaa aaataagata agaagtaaaa
      841 cagataatca gttaatgaat ttatatcatc aaacaactag aaagtttata gacttttgtg
      901 tagagcagaa ggtatgtgaa atagttcttg gggatataaa aggggttgaa aaagatacta
      961 agaaaaagaa aagattaaac agagttaata gacagaaaat atctcaaatg gagtatggtc
     1021 gaataaagga ctatataaaa tataaagcaa aagagcaagg tattgaagtt aagttagtta
     1081 aagaaaacta cacttctcaa acttgcccaa agtgttctaa aaaacacaaa ccaactggta
     1141 gaacttatag ttgtacttgt ggttatgaaa ctcatagaga tatagttggt gcttggaata
     1201 ttttaaataa aaagcataaa tatggtttag tagattttag aataaatcat aagcaaccaa
     1261 taaatttaaa agtatctacc gtttgagcca tgtgtgggcg aataacttta gggtagtagt
     1321 ggcgaagaca ccgtccgaag tagcttggct ttgtaaccac ctctgttgta ctgtttaagt
     1381 ataaataagt gtgttagcca ccaagaatcc ccttgcttta gctatgggga gtgtgtcaaa
     1441 tattaaacat agcttttata catttaataa ggtatgtaaa atctatgtaa aaagtaagaa
     1501 atataaacat ttttttaatt atattttaaa aatatcaagc aagttttctt cacaaatgaa
     1561 taaaaaatgt tataatatga ttaaagaaaa agataaataa ttagcaagat ttgtttagct
     1621 aaaattaaca agggagcgat aaattatggg tataacatta tcaaaaggcc aaaaggttga
     1681 tttaactaaa ggaaatccag gtcttaaaaa tattttagta ggtttaggat gggatactaa
     1741 caagtatgat ggtggatttg actttgattt agatacagca gcatttttaa caggagcaag
     1801 tggaagtgtt actaatgatg gagattttgt attttacaac aatttaaaac atacatctgg
     1861 agcagttgag catctaggag ataacagaac tggcgaaggt gatggagatg atgaacaaat
     1921 agttgttgat ttatctaaga ttcctggaga aataagcaag atttctttta ctgttacaat
     1981 tcatgatgca acagaaagaa gacaaaattt tggacaagta agtaattctt atataagaat
     2041 agtagataaa gacacaaatg aagagttaat aaagtatgaa ttaggagaag attttagtat
     2101 agaaacagct atagtagtag ctgaaatata taagcacaat ggagaatgga agtttaatgc
     2161 actaggttct ggctttgaag gtggtcttgc tgcattatgt ggaaactttg gaataaactt
     2221 ataatttcaa ctataacaat aaactaaagg gggatatttt ccatggctat agtattaaaa
     2281 aaaggacaaa aaatcgattt aactaaggga aatccaggtc ttaaaaatat aaaacttgga
     2341 ctaggatggg atacaaattc atttgatagt ggctatgatt atgatttaga tgtaagtgtt
     2401 ttcatggttg gagaatctca aagagttgaa aaagatgaag attttatatt ctacaataac
     2461 ttaaaacatc catctggagc agttgaacac ttaggtgata atagaactgg tgaaggtgat
     2521 ggagatgatg aggaaatatt agtgaattta gagacaatgc caaagcatat acaaaagata
     2581 gctgtaacag ttacaatata tgaggctgct gaaagaagac aaaattttgg acaagtaagt
     2641 aactcatata taagagtatt aaattctgaa aatgatgaag aaatattaag atatgactta
     2701 ggtgaagaat ttagtataga aactgcaatt acagtatgtg agatatataa gtataatgga
     2761 gaatggaagt ttagtgcagt tggagcagga tttgaaggtg gcttagaagc actttgtaga
     2821 aactatggct taaatgttta atttaagcaa tataatatat taaatataaa taacctatac
     2881 aaataatgta taggttatat tatataaaat tttgtggagg tttagtatgt cgattaattt
     2941 atcaaaaggt gataaaatag atttaaaaaa atctaaccca ggactgagta acatacttgt
     3001 aggtctagga tgggaccctg tgcaacaatc tggtggaggt ttttttaaat cgttatttgg
     3061 tggaggacaa gctgatatag attgtgatgc atctgtattt atgcttaatc aagaaggtaa
     3121 attaagtgga ataaaagatt taatttattt tggaaatcta aaaagtgcct gtaaatcagt
     3181 tttgcataca ggagacaatc taactggtga gggtacaggt gatgatgaac aaatcctagt
     3241 taatttggat aaagttccat caaatattca taaattattg tttgtagtaa atatatacaa
     3301 ttgtgtagat agaaaacaac actttggaat gatagaaaat gcctatataa gagttgaaga
     3361 ccaaggtaat aagaaagaga tagcaaaata caatctatct gataattatt ctgaaaaaac
     3421 tactcttata gtaggagcta tatatagaaa agatggaagt tggcaattta aagcaattgg
     3481 tgaaggaaca aaagatgcgg gtcttaaaga agtaatgcaa aatttagaca ggattgagtg
     3541 tgcatatgga atttaataat gatatcaaat attataatgt agataaagaa gaagttacaa
     3601 aagacttatc tgttaatcct gaaaaaggtt tatcagaatc agaagtcaag actagaagag
     3661 aaaaatatgg tttgaatgaa tttactccaa aagaagaggg tagtttttgg gatgatttaa
     3721 aggaaagttt aagtgaacca atgatagtta tcttgattat tgcagcagta gtaagtgcag
     3781 taattgggga aactcatgat gcaataggga ttgtaggtgc tatagcaata ggtatagcaa
     3841 taggtatgat tacagaaggt cgttctaaga aagcagcaga ggctctttct aagttgactg
     3901 aaaacataga agtaaaagta ttgagagatg gggaagtaca tcaaatttct aagagtgaat
     3961 tggtaccagg tgatatagta tatattgaaa ctggtgacat ggtacctgct gatggtagac
     4021 ttatagaaag tataaacctt aaaattagag aagatatgtt aacaggagaa tctgatgacg
     4081 tatctaaaaa gtgtgatgta gtagtttcta tggaagagat agaatctaaa ggagcagtag
     4141 tagtacagga gccaatacct gcaaaacaga taaatatggt gtttggtggc acacttgtag
     4201 cttatggtag aggagctcta gttgtaactt caattggaga taatagtgaa atgggtaaaa
     4261 ttgctcaaaa cttatcagaa acagatgagc aaactccact tcagataaaa cttggaaatt
     4321 taggtggttt gatagccaaa gtttctagtg cagtagcagg attactattt atatttatgg
     4381 ttttccaaat gataagtaag aatgtattaa atgtagatat gtctggaata ttaccgtttt
     4441 tagaatcaat agacccagtt aaaacagcat ttacagtatg tgttgcgttg atagttgcag
     4501 cagtgccaga aggtcttcca actatgataa atatgacact tgcaataaca atgcaaaaaa
     4561 tggctaaaat aaatgcattg gttactaaaa aagaagcttg tgaaacgata ggttctgttt
     4621 ctgtaatatg ttcagataaa actggtacat taactgaaaa tagaatgact gtagaagttg
     4681 catatgttga tggcagatat attgaaagtt cagaagagga aataaatagt tattttgaag
     4741 aaaactgtat gataaactct acagctgatg tagagcacaa tgatggtgat attaagtatt
     4801 taggaagtgc tactgaatgt gcattgcttt tatattacaa aaacgtagat tatagacaag
     4861 ttagaaaaaa ttctaatata gtagctcaaa atccttttac ttcagattca aagagaatga
     4921 cttctgttat aggtcaagat aatcatcatg tactattatc aaaaggagca ccagaagtct
     4981 tacttgaatt atgttcacat attcaaagag gaaaagacat agttccatta actgaaaata
     5041 taaaacatga gattttagaa gaaataaaga aattgcaaat aaaatcaatg agaactttag
     5101 ggtttgcata taaagaaatc tcacaagctg aagaagaagc agcagtaact gctgaaagtg
     5161 atgctatgaa tgtaagtgca atggaaaaca atttagtatt tagtggtttt gttggtataa
     5221 gagacccact tagaaaagat gttatagagt ctgtaaatac agcaaataaa gctggtgttt
     5281 ctgtaaaaat gcttacagga gataatataa atacggcaag agctataggt gaagagttag
     5341 gcctattaaa aaataatatg agagtggttg aagcttcata tatagatact ttagatgatg
     5401 aagaattaaa acaagagata caaagtattt caatcgtggc aagaagtaaa ccagatagta
     5461 aaatgagaat agtttcagct ctgcaaaaga gcggagatgt tgttgcagta acaggtgatg
     5521 gtataaatga tgctccagca ctttcaaaag cagatgttgg tatagcaatg ggtatatctg
     5581 gtacagaggt atctaaaaac gcagcagaca taattttaac tgatgatagt tttagtacca
     5641 ttgtaaaagg tattaaatgg ggtagaggaa tttatgataa cttccaaaga ttcgttcaat
     5701 tccaattaac tgtaaatgta attgcatttt tagtggctat aatatctcaa gttatgggac
     5761 aagagatgcc atttactaca atacaattat tatgggtaaa catcattatg gatggaccac
     5821 cagcactagc attaggttta gagccagtta gggaccatgt gttaaataga aaacctgttg
     5881 atagaaaagc caatataatt tctaagtcaa tggtgtatac aatagtatta aatgcatttt
     5941 atataacagc aatactaatg ttacaatcta catttaattt cttaggagtt gatgaagcgc
     6001 ataagggaac agtaatgttt gcgttatttg cttttagtgc actgtttaat gcatttaact
     6061 gtagagagtt taatgcagat agtatcttcc ctaattttac acataacaaa ttagcactac
     6121 aaataatagt gttgacagga attgcacaag taatatttac tcagctattc caagatttct
     6181 ttaactctgt agcaatggat tttatgacat gggtaaaagt tttatgtgta gcagcaagtg
     6241 taattgtggt taatgaagtt gtaaaatgta taataagact ggtaaaacta aaaaagactg
     6301 cttaaaacaa attttttaga agaaaacaga ctgactcaaa atatgaattt tgggtcagtt
     6361 ctgaataaac atttaaattg ggaggatagt tatgggtgac tcaaaggtat ttgaaaaaat
     6421 attctccagc caatcagata gaggaaatta cactccttca aagggatatc taagttattt
     6481 tatttcgtat attggtttag aagatgaagt tttgtacaat ctcgagatat ttaagacaaa
     6541 acaaaatatt gatagcaaaa aagatattgc tttatttact gatgttatag caaatcctag
     6601 tgattttgat attataaatt attttaaaag tggacttcaa aaatatagaa ctagcatgga
     6661 agatgtagat ataaatattt tgggatttga agagattgat tataaaataa agcaagcaat
     6721 ggatagagtg ttaaaagaag aggaaaaaga atttactaat gatagagtta aacaaaattt
     6781 tatagtgaaa ataatggcat ggataaaaat ttatatcggt gctttagata taaataaaaa
     6841 tgaagctcca aaagtaatat tttatggaga tataaaaaaa catgaagttt atttactctt
     6901 aatattatac ttggcaggtt ttgatgtttt atatcttaat ccaaattcaa agagtaatat
     6961 agatatttta aaatcagaaa gatacaatat agagtttgaa gaagccaaca taatagaaga
     7021 aaaaatttct tttgaagaaa gggttatttt aggggaaaaa atagataaaa gttctgtaaa
     7081 aaaagctttt actgttgggg cagaagcatc aaagcgtata agtgaggaat tgttaaatga
     7141 tgcaggattt ataaaacctt ggcaactcca agatagaaaa ataaaaaatc tacttctaag
     7201 ttcaactgtt gatgaaatat caatatactg gaatcaacca cttaaattaa gaccaggatt
     7261 taagtttaat gatgctattg tagaagctcc aaattttctt tctaagatta atggaatata
     7321 taacgataag aatgaatata ttaagttttt agatttatta agagattcgg aaagttcaac
     7381 atttattgaa tttaatggag atgtagatag attttcaaaa gcttttacaa gagaagcttt
     7441 tagtttatcc tttttattgg attcaaaagg tgcaatagat aaaaactctg ttttaaataa
     7501 taaagattat tccatatcaa cactagcatt gaatcaacaa attatgattt tagagaaagt
     7561 ggaagagttg ttagaaggaa gtatgttttt gaatggttta agtggagaag acaagataaa
     7621 aggtctattt acagtacttc acatggataa gaaatttgtt catatgatga acaatttcga
     7681 ttactcactt ataaacccaa aattgattat atacatgtac aagagtattg tatttgataa
     7741 agaaatagtt tttcttatgc ttttacttag taagattgga tttgatatta tcatattatg
     7801 tccaggagga gaaaacaata tagagaatgt tataaataac caattgattg atgtacacag
     7861 attggataaa atggtatatg accttaaatt aaattcgctt gaaaatgata tacctttact
     7921 taaaaaaatt tttggaaaga ggagacgatt ttagatgggg ataaatataa atagtttttc
     7981 aaaagataag aactctgtag aggttactga aaatttgcct acagcagttc aagaagaaaa
     8041 cttcgatata atggaatata caaataataa aaaaaatgaa ctgagaaagt ctaaggaagt
     8101 tgaggcattg acatctctta tagaagtaga aaatccagat acgattttac agtttggtag
     8161 gaaagcatct gaaggagtag caagagtttc tgatagcctt ttaaatacaa taaaattaaa
     8221 tagaaatgaa gaaaattcta aaatgttggt tcatttaact aaaataatgg acaagtttga
     8281 tttagacgat tttcaagaaa ctaaggaacc aaatttcgtt caaaaacttt tcaaaaaagc
     8341 taataatgcc attgaaatga tgtttcaaaa gtatgaaaca ttgggaggag aagttgagaa
     8401 aattcaaatt gagcttgaac aatacgaaag agatatagca ctttctaata agcaaatagg
     8461 tgctatgtta aatgaaaact ttgagtttta caatgaactt caaaaatata ttgttgctgg
     8521 agaaatggca atagaagaaa tggataatga aatactacct ttctttaaac aaaaatcaga
     8581 gactagtgga gaccaaatgg atgttgtcaa ttatcaagaa ctattaaaag tttatgatat
     8641 gttaaatcaa agagtctatg atttaaggat agcagaaaat atagctatcc aaacaatacc
     8701 tatgcttaga ggtatgcaac ataataatta tggtttaatt agaaaaataa atagtgcatt
     8761 tgtcgtaact ttaccagtat ttaaacaatg tttatctcaa gctatattac ttaaaaagca
     8821 agaattacaa gcaaaatctc taaaagcact tgatgacaaa actaatgaat tattgcttag
     8881 aaatgcacaa aatgtaagta ctcaaagtgc acaaatagct agaatggctg gaacaagttc
     8941 tgttcaaata gaaacattgg aaaaaatgta caatactatc aagtctggta tagatgaaac
     9001 tatgagaata gaagaaaaca atagagcttt aattaaggat aatacaaaga gattggaaga
     9061 attaaatacg actataattt ataataagta gtattatatt aagggctaat ttgtataaag
     9121 ttggcccttt ctatgtatat gaggaggcta gacattgaaa tatttcgatt atatctgcaa
     9181 agatgatttg gagagaattt ttttgaagga gccagaagat tttagtgcta agactgaaaa
     9241 agatgtgttg aaatatgcac tgggcgcttt tttatacgtt ccagctactc aatataatat
     9301 gatttataag agtattatag gggatgttaa gggagttaga ccattagcta tttgtttaga
     9361 agatgctgtt ggagtaaatg gagagttgga ggctatagaa aatcttagat taattttaaa
     9421 aaatattagc aatgaaagta taactaataa agatggaata ccactaattt ttgtaagaat
     9481 aaaagatgtg gagcaactgt tgagaattaa agagattata attaaaaata gtcattttat
     9541 aacaggaata ttaattccta aagcaaattc ggagttaatt gaaaattgta tagaagcttt
     9601 agattttatg aatcttcaag atatgtatgt tattccgata gtagagacaa aagaatttat
     9661 atataatgaa aaaaaagaac ttagttttac taatttgtac aatgctattt taagacataa
     9721 gtcaagaata ttaagcatta gagtaggcat aactgatata ttagggatgt atggtataag
     9781 gagagataaa aacttttcta tatataataa tttgatttgt tcttcattta tattagatat
     9841 aatcacatat cttcaaagac cagagttaga tatacctata agtggtggag tatcagaatt
     9901 ttttgatatg acgaataagg atataagaaa taagtatata gaagaaatat tattggataa
     9961 atatcatgga ttaattggaa agaccgtaat tcatccaatg caaattcaaa ttgtacaagc
    10021 gctatctaca gtatcatatg aagattttac tgatgctttg gatatactgg atagtacaga
    10081 cagtaagtat ggtgtgagca agggtgtatt aggagagaga atgaatgaaa caaatccaca
    10141 ttttttatgg gctaaaaaaa cattgattct atctaaaata tatggtgtat taaataaagg
    10201 agtagactat gaagaattgc ttaaatttta attgtgagat tgacattatt gataatccat
    10261 tgaatttaga actaaatgat tttttagacg taggaataag aaataatagc aaaagacgtt
    10321 ttttgtttat ctcaaagaaa ctcggtaaac atttagcatg taagccaagt gagatggata
    10381 atttgggaaa attaatggct acaatttata atgaaaaaaa taaggaagaa tcaaatggta
    10441 ctgttatagc ttttgctgaa actggtacag ctgttgggca tagttttttt gattacttat
    10501 gtggagatta tgaatttatt catactacta gagagcagtt tgacgagtta gaaaatctgg
    10561 aatttttaga ggaacattcc catgcaacag accagaattt atactttggt atgcttaaca
    10621 actttaaatt tggagatgag ataatattgg ttgatgatga gattaccaca gccaatacat
    10681 gcatgaatat aattaggaaa attcaaagca tttatcctaa aaagaaatac actatatgct
    10741 caatactcaa ttgggtaagt gatgaaaatt taagtaaagt tactcgttta gaaagagagc
    10801 tagattgtaa gatagatttt gtatacttat ttaaaggaaa ctttaaattt tatatagatg
    10861 aagatattat ttcaaattac aacgataatt atagtattac taaattagag actatttcaa
    10921 gtgataaagt aaatttagta gtaaatcata tttatgttga tttagaagat tatattggaa
    10981 ataagaaata tgtaaaatat acaggaagat ttggaattaa tagaagagaa caatttagat
    11041 taaaagaaat cgttaagaga gaaagtaaaa aaataactat ggccagtaaa gaatctttaa
    11101 aaaatgaaaa agaaaagatt ctatttttag gtactgaaga atttatgtat atacctatgc
    11161 tgtttgcaaa agaaaaagaa aatcaatatg atatatacta tcattcaaca acaagaagtc
    11221 caattattaa tattgataag aataattatc ctattaaaag taaatttatg ctaaagagtt
    11281 tttataatat agatattcaa aattatattt acaatataga taaacataat ttttctaagt
    11341 gttatttatt tgtagagctg aataaaagtg aaaattctta tctagaa
//
LOCUS       DAGSHT010000109        11338 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.111, whole genome shotgun sequence.
ACCESSION   DAGSHT010000109 DAGSHT010000000
VERSION     DAGSHT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.111"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     regulatory      <1..74
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            257..829
                     /locus_tag="MJB72_002453"
     CDS             257..829
                     /locus_tag="MJB72_002453"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBY8263539.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            893..1543
                     /gene="fsa"
                     /locus_tag="MJB72_002454"
     CDS             893..1543
                     /gene="fsa"
                     /locus_tag="MJB72_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBY8263540.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            1695..4619
                     /locus_tag="MJB72_002455"
     CDS             1695..4619
                     /locus_tag="MJB72_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8263541.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            5211..5669
                     /locus_tag="MJB72_002456"
     CDS             5211..5669
                     /locus_tag="MJB72_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263542.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            5730..6005
                     /locus_tag="MJB72_002457"
     CDS             5730..6005
                     /locus_tag="MJB72_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8263543.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            6064..7338
                     /locus_tag="MJB72_002458"
     CDS             6064..7338
                     /locus_tag="MJB72_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY8263544.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            7468..8520
                     /locus_tag="MJB72_002459"
     CDS             7468..8520
                     /locus_tag="MJB72_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY8263545.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            8520..9551
                     /locus_tag="MJB72_002460"
     CDS             8520..9551
                     /locus_tag="MJB72_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBY8263546.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            9643..10455
                     /locus_tag="MJB72_002461"
     CDS             9643..10455
                     /locus_tag="MJB72_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBY8263547.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            10458..>11338
                     /locus_tag="MJB72_002462"
     CDS             10458..>11338
                     /locus_tag="MJB72_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBY8263548.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLT"
BASE COUNT         4572 a         1197 c         2203 g         3366 t
ORIGIN      
        1 gtttgataat atggttcaaa tgtttctact aagctaccat taatagcttg actacaggag
       61 tgaagaagat atagtttata cttattttaa attatatatt aagttaatta ctttgaattt
      121 taagctcctg tctatgatat agacaggagc ttttttagta ttttaatata aaatacttac
      181 aagaatagat agcaaaatta gaacaaatat aattttttta ttaatataat tttagaaagg
      241 gctaggtgga agttcaatga aggctttaca agaaaagata ttgagagaag gtagtgtttc
      301 aggaaatgat atactaaaag ttgatagttt tttaaaccat caaattgatg tagctttttt
      361 aaatgaaata ggaagagaat ttaaagagag atttaaagga gaaaaggtgg ataagatatt
      421 tacaatagag gcttcaggaa tagctatagc atcaattgtt tcacagtatt ttgataatgc
      481 acctgtagta tttgcaaaaa aatcagaatc taaaaatttg gataaagatg tatatgaaac
      541 taatgtatat tcatttacta aggcaagaga gtattcagtt aaagtttcta aaaagtacat
      601 aaacaaaggc gaaaatatat tgatagtgga tgattttttg gcaaatggaa gagctgctct
      661 tggacttaaa gatttaattg agcaagcaga agctaactta gttggagtag gaattgtcat
      721 tgaaaaagga tttcaagctg gaggagcact tttaaaggct aatgatgtaa gattagagtc
      781 tttggcagta gttgaatcta tagataatgg aactgtaaaa tttaggtaat ttatgatatt
      841 atatatttgt gtttagcaca tattttaact atgtaattat ggaggatttt ttatgagatt
      901 ttttatagac acagccaata ttgaagaaat aaaagaagca aatgatttag gtgtaatctg
      961 tggtgtaact actaatccgt ctctaatagc taaagaaggt agagatttta tagaggtagt
     1021 taaagaaata agtgaaatag tagatggacc aataagtgca gaagtaataa gtttagaaca
     1081 taaaggtatg atagaggaag cagaaaaatt atctaagata cataaaaata tagttataaa
     1141 gatacctatg acagcagaag gtctaaaagc ggtgaaagta ctatcaagta aaggtataaa
     1201 gactaatgta acgcttatat tctctgctgg acaagctctt ttagctgcta gagctggagc
     1261 tacatatgta agtccttttg ttggtagatt ggatgatata tcacaaaatg gtcttgattt
     1321 aattgaagaa atcgttgata tatttagtgt aaatagtatt gaggctcaaa taatagttgc
     1381 tagtgttaga aatccaatac atgttttaca agctgctaga atgggagcag atatagcaac
     1441 agttcctctt aaagttataa accaaatgat taagcatcct ttgacagaca aaggaataga
     1501 tagctttatg aaggattggg aaggtgcttc tttaaaatta taattataaa ctacgcttta
     1561 taagtatatt aaagaggtta ttatggaaat tctaagtaat aaggctaggc tattttgcct
     1621 agtcttattt tggtgtataa tatattgaaa catattattg aaatagatta attttttaag
     1681 tgaggaggcg aaaaatggat aaaatattaa gagagataca aagagaagat aaaaaaaatc
     1741 catatactga ccaagaactt gctgaaatgt taaatgtagc taggtctgaa gtgatatcag
     1801 ttaggaaaaa aaataatatt ttagattcga gagaaagaag aaaaaaaata ttaataaaag
     1861 atatatcaaa aattgtaagt gaaaatcctc aaatgtctga aagaaaaata actgaaatat
     1921 taatagaaaa tggatataat atatcgagaa gtgcagtttc aaaattatta aaagaggaaa
     1981 atttaaatca gtcaaagatt gggattaagg ataaagaccc agcatcatca ggaaagtatg
     2041 tgaaggtgag taaagttgta gaagaagatg gatttgaaga attgataggt ataaagggaa
     2101 gtttgaaaga aaaggtaaat ttagctaagt cagcaataat gtatcctcct aatggccttc
     2161 atactataat atatggagaa acaggagtag gtaaaagtga attagctaca tgtatgtata
     2221 aatatgctat aaaaaacaac attaaagaag agaacagccc atttatagtg tttaactgtg
     2281 cagactatgc agaaaatcct aatctattga tagctcaact ctttggggta gtaaaaggtg
     2341 catatacagg agcagatgca aatagagaag gtcttgtaga acaagctaat aatggaatat
     2401 tgtttttaga tgaaattcat agattgcctg ctgctgggca agaaatctta ttttcattaa
     2461 tagatagagg agaatttaga aggttaggag aaagtagttc taatagaaag gcaaatgttc
     2521 tcataataag tgctacaaca gaaaatccag attctaattt acttcaaaca tttagaagac
     2581 gtataccaat ggtaataaat ttacctaata tgagtgaaag accaaaatct gaaagatatg
     2641 aaataataat aaagtttttt gaaagagaag caaagcgagt aaacaagaat tttataataa
     2701 ctaaagaagt tatgtgtgca cttatgaatt ataagtgtac tggaaatata ggtcagttaa
     2761 agagtgatat acaagtaact tgtgctagag cttttagtaa atcgatattt tctagtgata
     2821 aagtaattat agatttagat agtttaaggg attatataaa aagtggatat tatgattgta
     2881 attgtaaaaa tgaaaagtat tctgagtttt cagttgagga tatatatata gatattagtg
     2941 aattggtgag tggaaaaaat agaattttat ctgataatga gataagcgag atatataatt
     3001 atgcagaaaa agagcttaag gttttggaaa gtaagtgctt ttcagaagaa gagttaaaaa
     3061 atgcatttat agaaaagtta gataagaagt ttaatgctat taaaaacaat aaaaatttat
     3121 ctgatagaag aagtcgaata gactggggag gacaattaaa agagtctaca ctggagataa
     3181 tggataaggt aattgtattt attaaggagc agtttaagca tgtaaatcaa gggctgtact
     3241 tggctctagc aattcatatt gagcatgcta taggtaggat aaaagatgga aaaactataa
     3301 taaatccgag tttagataaa ataaaagcat caatgccaat ggaatatgag ttgtcaagat
     3361 atataatggg tatagtagaa gatttgacac aagtaacatt tccagaagat gagcttggat
     3421 atttggctta ttatataaat aaattttgtt ataatgagga aagtataaaa gataaagtta
     3481 aagtagtaat agttacgcat ggtaaagttg gaattgaaat gtctaaagtt gtaaatcata
     3541 ttttaggtat agaatgtaca ctaggtatag aaatagcact tacagattca ccatcagagg
     3601 ggatagagca tgtattagaa gagcttcaaa agatagaagc tagaaaaggt attctagttc
     3661 taattgatat gggttctttg gtaattcttg gtgatgaggt tgaaaaaagg cttggaatta
     3721 gatgtaaaac tgtaaataga gtagatacat tattggcaat ggaagcagga aaacttgcaa
     3781 ccattgaagg gaaaagttta gatggtataa tagctgattt aaagaaaaat aagaattatg
     3841 ctatgataaa tacaaataaa tttagttaca gaaaaaacga gtatggaaag aaaaatgtaa
     3901 tcattacttt atgtttgagt ggtgtaggta cagctttaaa tttaaaagag catatagaaa
     3961 aacaaattaa ggaatatgac actttaattg aagtaaagcc agttgctttt ttaaataata
     4021 atttagaaga agagttaaat gatattgaag ctgaatataa tattattgct atctgtggaa
     4081 ctattgatat agattataaa aacgtgcctt ttatttctta taatgaggta cttggtaaaa
     4141 atggtattaa taaagtatta gagcatttaa atagtaaaca accagcttta aatattgaaa
     4201 accagttgaa taatttaata catgaagatt tgattttata caattttgaa ggaatttcaa
     4261 aggagtatat aattgatact cttgtttcaa aacttgaaga aggtggatat gtcgactcaa
     4321 agtatatatt aagtgtatat aaaagagaag caatgggaag tgttgtaatg gcctctaagg
     4381 tagccgtgcc acatggactt ccagaaaacg ttataaagcc tgcaatagca attgcgaggt
     4441 tgaataaacc tatagtatgg gacaataaat ttatggtgga tttggtggta ctactggcac
     4501 ttaaagaaaa taataagaaa gaaatacgaa gcttattttc aaagattaat gaccagcata
     4561 ctttagaagt tttattaaat actgaagata aaaatgaaat aaaagaactt ttaagctaat
     4621 tgttagtacc gagaagaaag acaaaaaaat ttgctttagt agtataaaat ttaaaactac
     4681 tcaattgcta agtagttaat tactctatat aaggctagtt ctatgaaatt tatttatatg
     4741 attttataga attagctttt ttcttggtaa ataaaatata tgaatttatt tatgtaaaaa
     4801 gtaaatttta ttggacaatt ctataaaaat ttttcctata tttcttataa aatatagaat
     4861 atataaaaaa tatataaaaa taaattaaat tcataataaa taattttata aaatagttgt
     4921 taaacataaa aaaccatttg taaaaaacaa aaaaatcatt tctctaacaa aaaaaaccac
     4981 aaaaaatata aaaaagtatg gctagttaag tgattaacaa gtaaaaatta aaataaaaat
     5041 caaaaaaaat ttatcaatga aagtcagcta aaatcctgga agagagcatt atttttggga
     5101 aaacattttc ttttgtttca caaaaaaagc cactttttgg cacacaacat gctttatata
     5161 aagtcaagaa aaactaaggt ataagacaaa ggcaattagg aggtgtaaat atggagttta
     5221 taaattttgt aagtgataaa gttatagacc ttaaaatgga ttgtaaaacg aaagaagatt
     5281 tctttaaaga aatacataat aaagtttttg atttaggctt tgtaaaagaa gaatttggag
     5341 aaaaaatatt agcaagagaa aatgtatttc caacaggttt aaatttaggt gattatggag
     5401 tagcaatacc acatacagat gcggaatata ttaaagagca atttatatca gtctgtactt
     5461 ttaatgaacc agtagtattt agttctatgg aagaccaaga tgaaaaagta cctgtaaatt
     5521 tggctttcgt attaggactt aatcaaccac acagtcaatt atctgtatta acagaattaa
     5581 tgggaattat gcaaaataaa gagttagttg ataagttgat gagttctact gataaagaag
     5641 aagttttaaa gacactaaag tctttataaa aataaaaaag tactaaaaat aaaaaatata
     5701 aaaataaaaa ttattaggag gaaataaaaa tgaaaaaaat attagtagca tgtggagcag
     5761 gaatagcaac atcaacagta gtttgtgaca aggtagagag attagttaag gaaaataata
     5821 tacaagcaga agttatacaa tgcaaaatag ctgaatgtgc aacaaagcaa gaaggagcag
     5881 atttaatagt atcaacaact atactaccaa caacttataa tataccagca ataaaagcaa
     5941 cagcatatat aactggagta aatacagcag cattagataa aaaaatatta gatgctttaa
     6001 aataatccta tcatagatac attgaaataa tattttataa aacagaaaaa ggaggattta
     6061 taaatggata aattattagc agtagtacaa tacattttaa atatgggacc gacagtaata
     6121 ctaccaataa caattgtagt actaggaatg atatttagaa ttaagtttac aaaagcttta
     6181 aagtcaggtc ttacaattgg tataggattt gtgggtataa acttagttgt aaacctatta
     6241 acatcaacac tagggccagc ggctcaagga atggttgaaa gattcggact taatttaaca
     6301 gtaatagatg ttggttggcc aacaacagca tcagcaacat gggcttcacc agttgcacca
     6361 atattaatac cagtttgttt attagtaaac ttactattat tatacttcaa taaaacaaag
     6421 atattaaacg ttgatatatg gaattattgg cactttatag tagcaggtgc agttggatat
     6481 atagtaacag gaagttatgt ttgggctata ttctgtgcaa tactaatgga gttattagta
     6541 ctgtttattg cagaaaagac agcaaaaaat gttcaggagt tttatgattt agaaggtata
     6601 gcattaccaa ctggttcaac agcttcattt gctccacttg gatatttagt aggtaaattt
     6661 gttgaaaaaa taccaggaat aaataaaata caagcagacc cagactctat acaaaaaaga
     6721 tttggtatat ttggagaacc aatgatgatg gggattatac taggtataat acttggagca
     6781 cttgcgggat atgatgtagc aggaatattc aatgtaggta tatcaatggg tgcagtaatg
     6841 ttacttatgc ctagaatggt tagaatatta atggaaggtt taattccaat ttcagaatca
     6901 ataagagatt tcttacaagc aaaatatgga gacagagaac tttatatagg tctggatgca
     6961 gcagtagcaa ctggacatcc agcagttata tcaacagctc ttatacttgt tcctataact
     7021 ttgttcttag cagttatact accaggaaac aaagttttac catttggaga cttagcgaca
     7081 ataccatttt atatggcatt tatagtgtgt tttagaaaag gtaatatagt tcaatcagtt
     7141 ataacaggaa caatagtaat agctatatca ctttatatgg ctacaaactt tgcagatgtt
     7201 catacacaat tattacaaca agcaaatatg atgccagaag gaacaacaat ggttacaagt
     7261 atagatacag gtggaaactt gctaaaatgg ggaatattga aattatctca attagtttct
     7321 tcatttggaa tattctagta tgtagttaat agagattact cttttttagt aaagtatgcg
     7381 aaaatttaac atttagcagc tactaaaaaa gagtaatatt ttttataaat ttttaaaaat
     7441 acaaaataca agaggaggag atttatcgtg aaagcagcag tattacatgg cacaaatgat
     7501 atgagatttg aagatataga aataaaacca tgtgaaagtg atgaagtaaa aataaaagtt
     7561 atggcagcag gaatatgtgg gtcagatcct ccaagagtat taaaacattg gaaatatcca
     7621 gttccagcta taccaggaca tgagttttct ggagtaattg ctgaagttgg taaggatgta
     7681 aaaaatgtaa aagttggaga tagagtagtt gcaattcctt ttataccttg taatgaatgt
     7741 gaatattgta aaagaggatt attttcattg tgtgatgacc atggaatgtt aggagctaaa
     7801 agttttggag cttttgcaga gtatgtaaat ataaaagcta caaatgtact tccaatagga
     7861 gatatggact ttgaagatgc tgcaatgata gagccactag cagttgctat gcatggagta
     7921 cttaatatag gtgttcaagt tggagacact gtagccgtaa tgggtagtgg aacaatgggg
     7981 cagttggtaa tacaaggact taaaatagca ggggcaggaa caattatagc agttgatata
     8041 tctgataata aattaagaga atcaaaggaa ttaggggctg atataataat taatgctaaa
     8101 gatattaacc cagtagaaaa aataaaagaa cttactggtg gaaaaggtgt tgacatagct
     8161 cttgaatgtg caggttcaaa aataactcaa gaacaatgtt tacttataac taaaaagaaa
     8221 agtaagatag gatttttagg gatagcatat tcagatataa ctttatcaga agaagcattt
     8281 gaaaatatat ttagaaaaga actagaatta aaaggttttt ggaactctta ttctgcacca
     8341 tttccaggac aagaatggac taagggaatc aatctagtta atgaaggaaa gataaaatta
     8401 aaagaaatgg tatctcatag attctcactt gaagatactt acaaagcatt tgaaatgata
     8461 agagatagaa aagaagaatt taacaaaata ttaattttac cacaaggggt tgaaaaataa
     8521 tgaaagcagt agttaaaact aaaccaggct atgataatgt agaagtttta gaagtagaag
     8581 aaccgaaagc tacaggagac aaggttaaaa taaaagtaga atatagtggt atatgtggtt
     8641 cagatataca ttcatttaaa ggtgaatatg caaatataaa agccccagta acattaggtc
     8701 atgaattttc tggaattgtt gtagaagtag gagaagatgt taaaaatata aaagtaggag
     8761 atagagttac atcagagact acttttgcaa cttgtgagaa atgtatatat tgtgaaacta
     8821 aagattataa cttatgccca actagaaaag gtattggtac tcaagtaaat ggaagttttg
     8881 ctgagtatgt attatcaaga gaagaaagca tacatgtact tcctgaaaat gtatctttat
     8941 tatcagcagc ccttacagag ccactagctt gttgtgttca tgcagctctt gaaaagacta
     9001 ctatagaaag ttctgataca gttttaataa taggaccagg accaattggt ttattattag
     9061 ctcaagttgt taaatctcaa ggtgctacag ttataatgtc tggtgttaca aaagataagg
     9121 aaagattaga aatagctaaa aatcttggag tagatagaac tgtaaatgta atggaagaaa
     9181 gtttggcaga tgtagtgaat gaaatgactg atggaaatgg agtaaacaaa ggatttgatt
     9241 gttctgggtt tatgccagca gttaacgaag ccttaagact gcttagaaaa aaaggtactt
     9301 tcgtacaagt tggaatcttt gctaaaaaat taaatgaaat ggaccaagaa gcaataatcc
     9361 aaagagagtt acaatatata ggaagtagat ctcaaaagcc aagttcttgg attacagcat
     9421 tagatttaat ggcaagtggg aaagtaaata cagaagcttt agttactaga actgttggtt
     9481 tagacgaatt taaagaaggt attacagctt taatgaacgg ggaagaaata aaagtagcag
     9541 taaaatctta aaaaaacatt tgtattattt taaaaaaaat tgtaaaatct actgtaggat
     9601 aaaaaaatgt aaggtcttaa aaaataacag gaggaagaaa gtatgagcac tcttagagat
     9661 cattctaata atattagaaa aaacataata aaaatggttg cagaagctaa aagcggacat
     9721 ccaggtggtt cattatcaat agctgatatg ttgacagtat tatattttga taagatgaac
     9781 gtatgtgcag aaaatccaaa aatggctgat agagatagat ttgttctatc aaaaggtcat
     9841 gcagcaccag ctttatatgc tacattagca gaaaaaggat ttttcccaga agaagaatta
     9901 ataacactaa gaaaatttgg ttctaagtta caaggtcacc cagacatgaa aaaagtagca
     9961 ggaatcgata tgtctactgg ttctcttggt caagggctat ctgcagcaaa tggtatggca
    10021 cttgcaggaa aattagataa caaagactat acagtatata ctatattagg tgatggagaa
    10081 atccaagaag gtcaaatatg ggaagctgca atgacagcat cacattacaa attagataat
    10141 ttaattgcat ttgttgactt aaatggatta caaatagatg gttctaatga agaagtaatg
    10201 aatgtaaatc cagtagatga caaattcaaa tcttttggat ggaatgtaat agtaataaat
    10261 ggacattctt ttgaagaaat aggaaatgca gttgatgaag ctaaaaaagt tacaggaaaa
    10321 ccaactgtaa taatagctaa aacagtaaaa ggtaaaggtg tttcatttat ggaaaataat
    10381 gctgcatggc atggaacagc tccaaatgca gaacaagcag agcaagcatt gaaagattta
    10441 gaaggaggtc tataggaatg ggaatagcaa ctagagaagc ttacggacaa gtgttaaaag
    10501 aacttgcaga aaataaagat atagtagtat tagatgcaga tttaggaaaa gctacaaaaa
    10561 gtatatcatt taaagaagtt gccccagata gattttttga catggggata gcagaaggtg
    10621 acatgattgg tacagcagca ggtcttgcta cttgtggaaa aatacctttt gcgagtactt
    10681 ttgcaatatt tgcagcagga agaggctatg agcaaataag aaactctgta gcatatccaa
    10741 atttaaatgt aaaaatagca gctactcatg caggtgtaac agtaggtgaa gatggtggta
    10801 gccatcaagc tatagaggat atatctctaa tgagaggtat accaaatatg gttgtattaa
    10861 atccagcaga tgctttagaa gcaagacaag ctattcttgc atctattgat tataatggac
    10921 ctgtttatat aagattaggt agagctgcaa ctcctgatgt aaacagtgaa aattataaat
    10981 ttgaaatagg aaaaggaact gttttaagag aaggttctga cataacagta atagctacag
    11041 gaataatggt agcaaaagcc ttagaagcag cagaagaact tgctaaagaa ggtataaatg
    11101 tagaagttgt taatatatct acaataaaac cattagatga agcactaata aaagaaactg
    11161 ctaagaaaac tggaaaagta gttacagcag aggagcacag tataataggt ggtttaggtt
    11221 cagctgtatg tgaagcttta gcagaaacta aagatgtagt agttagaaga attggtgtta
    11281 aagatgtatt tggtcaatca ggtacaccag ctgatttatt aaaacactat ggtttaac
//
LOCUS       DAGSHT010000110        11235 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.112, whole genome shotgun sequence.
ACCESSION   DAGSHT010000110 DAGSHT010000000
VERSION     DAGSHT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.112"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            31..1143
                     /locus_tag="MJB72_002463"
     CDS             31..1143
                     /locus_tag="MJB72_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBY8263549.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1397..3421
                     /locus_tag="MJB72_002464"
     CDS             1397..3421
                     /locus_tag="MJB72_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8263550.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3641..4327
                     /locus_tag="MJB72_002465"
     CDS             3641..4327
                     /locus_tag="MJB72_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBY8263551.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4422..5477
                     /locus_tag="MJB72_002466"
     CDS             4422..5477
                     /locus_tag="MJB72_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBY8263552.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5592..7148
                     /gene="murJ"
                     /locus_tag="MJB72_002467"
     CDS             5592..7148
                     /gene="murJ"
                     /locus_tag="MJB72_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBY8263553.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7194..8900
                     /locus_tag="MJB72_002468"
     CDS             7194..8900
                     /locus_tag="MJB72_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBY8263554.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="MJB72_002469"
     CDS             8922..9995
                     /locus_tag="MJB72_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBY8263555.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="MJB72_002470"
     CDS             10007..10729
                     /locus_tag="MJB72_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY8263556.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..>11235
                     /locus_tag="MJB72_002471"
     CDS             10745..>11235
                     /locus_tag="MJB72_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBY8263557.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVF"
BASE COUNT         4430 a         1236 c         2060 g         3509 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt tgtgctataa tgaaaaaaga tttacataat attgaattta
     1321 tgtaatataa aacaagctta agatgtatta aaaaagattt aaagatttaa agaaccaaaa
     1381 aattaggggg gatatgatga aaaaaacaac aaaattattg gcaacaggaa tgttgtctgt
     1441 agcaatggtg gcaccaaatg tagctttagc ggctgaaaat actactgcaa atacagaatc
     1501 taattcagat attaatataa atttacaaag aaaaagtgta gtgttaggaa gtaaatctaa
     1561 tgcaagtgta aaatttaaag aaaaattaaa tgctgattca ataacattaa attttatgtg
     1621 ttatgatatg ccacttgaag ctactttaaa ttacaatgaa aagacagatt catatgaagg
     1681 tgtaataaac tataacaaag acccagaata cttaaatgta tgggaacttc aaagcataaa
     1741 aataaatggt aaagatgaac agaaagtttt aaacaaagaa gacttagagt ctatgggatt
     1801 aaatcttaag gattatgatg ttacacaaga gtttataata agtgatgcaa attcaacaaa
     1861 agcagtaaat gaatatatga gaaaaacttc tgcaccagta aaaaaacttg ctggagctac
     1921 tagatttgaa actgctgttg agataagcaa acaaggttgg aaagatgggt ctagtaaggt
     1981 tgtaatagtt aatggagaat tagcagcaga tggaattaca gctactccac ttgcatctac
     2041 atatgacgca ccaatattac ttgcaaataa agatgatata ccagaaagta caaaagcaga
     2101 gttaaaacgt ctaaatccaa gtgatgtaat aattataggt gatgatggtt cagtttcaca
     2161 aaaggcagtg tctcaaatta aatctgctgt aaatgtaaat gtgactcgta ttggtggagt
     2221 tgatagacat gaaacatctt tacttatagc aaaagaaata gataagtatc atgatgttaa
     2281 taaaatatac atagcaaatg gatatgctgg tgaatatgat gctcttaata tttcatcaaa
     2341 agctggtgaa gaccaacaac ctataatatt agctaataaa gattctgtac cacaaggtac
     2401 atataattgg ttatcctcac aaggtttaga agaagcttat tacataggtg gtagtcaaag
     2461 tttatcatca aaaattatag accaaatatc taaaatagca aaaaatggta cttcaaaaaa
     2521 tagagtttct ggagcagata gacatgaaac aaatgcaaat gtaattaaga ctttctatcc
     2581 agataaagag ttgagtgcaa tgttagttgc aaaatctgac ataatagttg attcaataac
     2641 agcaggacct ttagcagcta aattaaaagc tccaatactt ataactccaa aaacttatgt
     2701 ttctgcatac catagtacaa acttatcaga aaaaacagca gaaacagtat atcaaattgg
     2761 tgatggaatg aaagatagtg ttataaattc tatagcatct agtttatcaa aacacaatgc
     2821 accaacagaa ccagataatt caggttcagc agcaggaaaa actgtagtga tagacccagg
     2881 tcatgggggt tctgattcag gggcaacaag tggattaaat ggtggagcac aagaaaagaa
     2941 atatactctt aatacagcat tagcaactac tgaatattta cgttcaaaag gtataaatgt
     3001 tgttatgact agagatacag ataaaacaat ggctttagga gaaagaacag cattatcaaa
     3061 tactataaag ccagatttat ttacaagtat acattacaat gcgtctaatg gatctggtaa
     3121 tggtgtggaa atttattaca aagtaaaaga taaaaatggt ggaacaacta agactgcagc
     3181 atcaaatatc ttaaaaagaa tactagaaaa atttaatatg aaaaatagag gaatcaagac
     3241 aagaacactt gataatggaa aagattacct atatgtgtta agaaataata attatccagc
     3301 aatacttgtt gaatgtgcat ttattgacaa taagagcgat atggataagt taaatactgc
     3361 agaaaaagta aaaacaatgg gaactcaaat tggaatagga atagaagata cagtaaaata
     3421 aaatagaaaa tattaaagca aaacatatca tctcctgtta taatgtaaaa tataacaaga
     3481 gatgatatat ttttgtaaag aatcaaattg tttacaatga tgtttcttgt attgataatc
     3541 tgctcattat tgagtataat atagaaataa gtcaaaaata agatagaaat aagttgaaat
     3601 taagaattaa aaaaattttt attccaatat ggaggtaact atgtctggat atactaatga
     3661 tgagtgtgaa atacctaaaa ttaaaagcta tccaggggca gataaagaaa ttgcaagtga
     3721 gattgactat agcatagtaa aaggaacagt tttattcgac ttgtaccaaa gaattatgga
     3781 tttagtttta tctataatag gacttgtgat aggtttacct ttaatagcaa tatttggaat
     3841 tcttattaaa atagaggaca aaggaccaat aacttataag caagaaagac taggaaaatg
     3901 cgggagaaga ttctatatat ataaattgag atctatgaga acagacgcag aaaaatttgg
     3961 cgcacaatgg gctgaaaaag atgatcctag aataactaag gttggaaagt ttattaggaa
     4021 aactagaatt gatgagattc cacagctgtt taatatatta aagggggaca tgggcttaat
     4081 aggtcctaga ccagagagac cgaactttac tgttcaattt aatgaggaaa tacctggatt
     4141 tatcaacagg ctcgctataa aacctgggct tacaggttgg gcacaagtaa atggtggata
     4201 cgaaatcact cctgaagaaa aacttaaaga agatatttac tatataaaaa ataggagtat
     4261 attacttgac tttaagatac tctttaaaac tgttaaagta gtattgacag gagacggagc
     4321 tagataattt taaaatatta gattactaat caatgaaaaa gctctttaaa aagagctttt
     4381 ttattgtcaa aaaatacaaa aaagaaaagg aatgagaact tatgattaaa aaaatttcta
     4441 ctatattatc gttagttttg ttaatctcta tttctagtac gataggagta tttgcagatg
     4501 caaatcccaa aagagaattg atagaaggga gtataccaga gatttcgaca gaattaaata
     4561 aaagagcatt taaggattct aaagaagtaa ttcttgtaaa tgaagagtca attgtagata
     4621 gtataagtgc aacaccttta gcatatgcaa aaaatgctcc aatagttgta actaagagta
     4681 aaaatcttgg tagagtaaca agaaattatc taaaagaact aggtccagag aaggttacga
     4741 tagtaggtgg tttaaaggca gtatctaaag atgctgaacg taatatagaa aaaatgggaa
     4801 tgaaagtaga aagaataaga gggaaagaca gatatgatac atctttaaag atagctagag
     4861 aaatgtatag aacagtagga tttgatgaag catttttact tagctcaaca acaggattag
     4921 agaacgccat atctgtatat tcttatgccg ctaaaagtgg aatgccaata atatgggcta
     4981 aagatgaagg gtttgaggaa caaattgatt tcttaaaagg caaaaatctt aaaaaaatat
     5041 atgcattagg tgattcaaaa gagtttattg cagagattga ttctaactta aaaaatatag
     5101 aaggaataaa gcaaataaat aagtcaagta caaatgttga tttaataaag aagttttatg
     5161 atgaaaaaga tataaagaaa atctatactg ctagattaga ctttggtagt agatctgatg
     5221 taaacgaata tatttctttg ggagtagtat cagcaaaaga aaatatgcca atactgattt
     5281 gtagtgataa cttaagtcgt gcacaagata aattcttgaa agatagcaat ataaatgatg
     5341 tagttgaagt tgggtataca gtaggtgatt atagtctatt taagagcata tttaatctaa
     5401 cattcttatc ttgtatagta ttaatactat tattactatt gattacattt agggcactta
     5461 gatatgagtc gaaatagttt ttgatttata aggaagcttt tcataggtta aatttactta
     5521 attagaataa tgtttataag cagttttaaa agttctactt ttagagttta aattagataa
     5581 ggagaaagaa gatgtcaaaa acagctaaag cagcattgtg gattatggca gccactatgt
     5641 tttcaaaagt attaggtttc cttagagaac ttgtacttgc aaatttctat gggacaggta
     5701 tgtatgcaga cgtatttgta ttaacattaa atatacctgg tttaatcata gcagtcatag
     5761 gctctgcagt tgctacaact tatataccta tgtactttga gacaaagaaa agactcggag
     5821 atgaaggtgc cttaaaattt actaataatg ttttaaatat atgctatata atggctatag
     5881 taatagctat tattggtctt ttatttacag agcaatttgt tacagtattt gcagcaggat
     5941 ttagaaacga ccctgctaag ttccaagcag caatattatt tactaaaata atgatttcag
     6001 gagttttatt cctgagtgga agtaagatat ttagttcgtt tttacaggtg aatgacagtt
     6061 ttgtaattcc tgggcttata ggaatcccat ataatattat aataatagca gcaatagcac
     6121 taagtgcagg taaaaatgta tggatactgc cagcaggagc actattggct atggcaagtc
     6181 agctattgtt tcaactgcca tttgcgttta agaagtccta caagtataag ccatatataa
     6241 atttaaagga tgagtccata aaagagttag tcaacctagt attgcctatg ttggtaggag
     6301 tagctgttgg acagttaaat atatttgttg accgattatt ggcaaccact ttgggtgatg
     6361 gaaagttgtc tgctcttaac tatgcaaata ggctaaatga gtttgttatg gcattatttg
     6421 ttacatctat aataacagtt atatatccta aactagcaaa aatgtctggt aaagataata
     6481 aagaaggatt tataagtact attgtaaaat cttcaaattg tattatattg gtggtgcttc
     6541 ctatatctat aggagctatt atattagcag agcctcttgt tagaatatta ttccaaaggg
     6601 gtaaatttga tgcgttatca acagacctta catctatagc gcttagatta tattcactag
     6661 gtttattggc ttgtggagta agagatgtct tatatagggc attttactct ctttcagata
     6721 caaaaacgcc aatgataaat ggaagtatag ctttaataat aaatatagtt cttaatttaa
     6781 tattgataag accattgggt catgcaggta ttgctatatc aactagtaca tcaaacataa
     6841 ttacagttat attattattt atatctctta aaaagaaaaa tggatatttt ggaggggata
     6901 aaattataaa gactggtcta aaaagtttgg tagcatctgg tgtgatggca gtagctactt
     6961 tattgatata taataatcta tatgcattta tgggttcagg aacaattaaa gaaattatat
     7021 cagttggagc tggagtatta ggtggagctt ctgtatatac tgtattgata gttttattta
     7081 aggtagaaga gatggattta gcttttgaat ttttaagaaa aggaaaacaa aaactactta
     7141 gaagatagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatacaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaagca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa gccagtaaga aaagtcttaa atgaatgtgg ctttgaaaat gtaatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa caatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggt tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat actatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tactagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgac aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcagcat gttttttaat
    11101 ggtagctata ttattattta atctatttcc ttcattgata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta tttta
//
LOCUS       DAGSHT010000111        11133 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGSHT010000111 DAGSHT010000000
VERSION     DAGSHT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.113"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..625
                     /locus_tag="MJB72_002472"
     CDS             <1..625
                     /locus_tag="MJB72_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263558.1"
                     /translation="SLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSD
                     RKFNIKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILD
                     LIVTIITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKI
                     NKINSKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            917..2236
                     /locus_tag="MJB72_002473"
     CDS             917..2236
                     /locus_tag="MJB72_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY8263559.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(2311..2739)
                     /locus_tag="MJB72_002474"
     CDS             complement(2311..2739)
                     /locus_tag="MJB72_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263560.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(2830..4206)
                     /gene="hydG"
                     /locus_tag="MJB72_002475"
     CDS             complement(2830..4206)
                     /gene="hydG"
                     /locus_tag="MJB72_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBY8263561.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(4207..5292)
                     /gene="hydE"
                     /locus_tag="MJB72_002476"
     CDS             complement(4207..5292)
                     /gene="hydE"
                     /locus_tag="MJB72_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBY8263562.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(5328..6536)
                     /gene="hydF"
                     /locus_tag="MJB72_002477"
     CDS             complement(5328..6536)
                     /gene="hydF"
                     /locus_tag="MJB72_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBY8263563.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(6551..6802)
                     /locus_tag="MJB72_002478"
     CDS             complement(6551..6802)
                     /locus_tag="MJB72_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBY8263564.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(7183..8223)
                     /locus_tag="MJB72_002479"
     CDS             complement(7183..8223)
                     /locus_tag="MJB72_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBY8263565.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
     gene            8693..9853
                     /locus_tag="MJB72_002480"
     CDS             8693..9853
                     /locus_tag="MJB72_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBY8263566.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            10195..10356
                     /locus_tag="MJB72_002481"
     CDS             10195..10356
                     /locus_tag="MJB72_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263567.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            10549..>11133
                     /locus_tag="MJB72_002482"
     CDS             10549..>11133
                     /locus_tag="MJB72_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBY8263568.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKEYTVE"
BASE COUNT         3943 a         1500 c         1635 g         4055 t
ORIGIN      
        1 atcactattg ggaagattta ataatgtagt aatagtattt ctattaggaa tgattttaac
       61 tacgatacta gaatatttta caggatttat tttagaaacc ttatttcatg ctaaatggtg
      121 ggattattct gacagaaaat tcaatataaa gggtagagta tgtttaaaaa atgcaatata
      181 ctttggtgta atgtctgttt taataataag atttatacat ccatttataa aatattttgt
      241 aagtataatt ccatatagaa tattaatttc aatggctata attacaacac tttggactat
      301 acttgatttg attgttacta ttataacact taaaaaatta gatataaagt taaacttatt
      361 agacgatata attacagacc ttaatgatat aaatgtgaaa ttggataaat ttgatagagg
      421 agaaatacaa gctttattta aaacaataaa taacgacgaa ttagaagtta gagaaaaaat
      481 aaacaaaata aatagtaagt tggaccgtat agagtctaat attattttac aaaaaagagt
      541 tataaaagct tttccacata taaaacataa aaaacaacaa gagcaactag aacattttaa
      601 aaaaattatt aatgagaaaa ggtaattata caatttaatg aagacgaata gaataatggt
      661 atttataaga tgattttcag aggactctat aaaaatagaa tagagtcctt tattttttga
      721 aaaataatgt ttatttgata aaaataaaat atattgtttc tgaataacaa atattttgaa
      781 aattataaaa acatatattg acaatttgtg tcaaaaatac taacattaag tatataaaga
      841 attttatatg agaatatctt tataaatcgc aataatttta aaacataaat ttatttaaag
      901 aggggatgat ttgccaatga gcgagataac tagtcaaatg ataagtacag aagaaaaaaa
      961 atatgttgct aaaactcaaa agatacctta ttatccagta gcatttaaat caggtgatgg
     1021 tgcaatgcta tatgattatg aaggtaatga atatgtagat tttttagcaa gtgcaggctc
     1081 tgcaaatgta ggtcatggta ataaggaaat ctcacaagca gttaaagaac aaatggatga
     1141 tataacacaa tatacacttg catatttcca tagtgatcct cctgtaaaat tagctgagaa
     1201 gcttgtggaa atagcccctg gagataatga taaaaaagta ctatacagtg caactggttc
     1261 tgcttgtata gatgctgcta taaaattagc aagagggtat actggaagaa caaagataat
     1321 atctatgtgt gaatcttatc atggaagtac ttatggagct atatctatat ctgctttaag
     1381 tacaaatatg agaagaaaaa tgggtccatt gttaccagaa gtttatcact tccattatcc
     1441 tgataaaaat agaacagcta aagaatgttt agatgagata gaatatgctt ttgcacacta
     1501 tctaccagca gaagaggtag ctgcaatatt tatagagcca attgcaggag atgcaggaat
     1561 aatagtgcct ccagtagagt gggtacaagg tttaagtaaa atatgtaaag aaaatggaat
     1621 attacttgta agtgatgaaa tacaacaagg tatgggaaga acaggtaagt ggtttggtat
     1681 agaaaatttt ggtgttgaag cagaccttat agttcttgga aaatctgttg gaggtggatt
     1741 acctctagga gcagttgttg gaagaacaga aataatgcaa agtcttgatg caccagcaca
     1801 tttatttact ttagctggaa acacaacagt atgtgtggct gctttaaaat ctatagagat
     1861 aattgaaaaa gaaaacttac ttcaaaagag tatagaaatg ggagattata ttaaagcagg
     1921 atttgaaaaa ctaaaagaaa aatacgatat aattggagaa ataagaggca taggattatc
     1981 tataggagtt gatatagtta aaggaaaagg ttctaatgaa aaacatccag atgctacagc
     2041 taagatatgt tatagatgta tacaaactgg attaataatg atattcttag gacaatctac
     2101 tttaagagtg caaccacctc tagtaataac taaggagcaa gtagataaag caatgaacat
     2161 catagattct gcaatagatg attatttaaa tggtagaatt ggtgatgaag tatatgaagt
     2221 aactcaaggt tggtaaaata aatattaaat gaaaatagga gttatctcaa ttttagaaaa
     2281 ttatttctga aatgagataa ctcttttaag ttacccacaa ataagaaaat tgtttgattc
     2341 attaaagact atatttcgga tgtcgttttt gtgagtatca gagacagtat tacgagaata
     2401 attaccttta ttcgtactat ctgttaattt agttataatt aagatagcta aaagaataag
     2461 aattactgtg tataaagcaa taacatatgg ttttctttta tatttgtatt tgttattgaa
     2521 aaaagaatga ccataattat ttttatttac tttagatgta tttgtattct ttttcttgtt
     2581 acctttcttt ttatcatcct tttttctatt tttagggtaa ggaatgactt tgtgtgtatt
     2641 atcaagtttg atatttttat ttttttgaat attgttttcc aggctagcct ttttttctcc
     2701 aaattgaata gttttattgg tattgttgtt atctttcaat gttaatctcc ctcccttaaa
     2761 ctttcccttt agataatatt actactaacc tatggaatat acaataaata atatataagt
     2821 aataaaattt tataggtata aatctctttc tccacgctct aaggcagtca tattgttaga
     2881 tactatgttt cttatatctt ctctttctat cttacctatt tcttctgcga taagtttttt
     2941 acccataatt ttaagttctt catctccata atcgattaaa aattcattta atgtagtaag
     3001 agcatttggt gtacatacat tatggatttg tcgactttta gctaagctca tgaatctatc
     3061 gccagttcta cctttacgat aacaagctgt acaataacta ggtatatatc catcagtaat
     3121 cagttcttta agcacctcaa cagggcttct atggtctcca acttcaaatt gttctgtatt
     3181 gtcaccatct tcatatgctt tatatcctcc aacacctgtt agagaacctg cactaatttg
     3241 tgatacacca tattttagta actcatttct catttcagca gtttctctag tggacattat
     3301 gattccagta aaaggaacag ctaatctagt tatagctact atttttttga acatatcgtc
     3361 agaaactaag tgaggaaaat cttctaagct cataccttca gcttttttca atctagggaa
     3421 agatatagta tggaaaccta caccaaattt ttcctctaag tgttcgttgt gcatcataag
     3481 tcctagaact tcaaatttag ggtcagataa accaaataaa actcctgcac caacatcatc
     3541 aacaccagct tccatagctc tatcaaaagc tgttaaatgg taataatagt cattttttat
     3601 agattgacca tgcattttta taaatgttgg tttgtgataa gtttcttgga ataatatata
     3661 agttcctatt cctttttctt ttagtaattt atactcatct acacttgtag ctgctatgtt
     3721 aacgtttacc cttcttatat ttccgttttc attatatgtt gaataaattg catctaagca
     3781 atctaagatg taatttatat cacaattttt agggtcttca ccagcttcta gagcaagtct
     3841 tttatgaccc attttttcta atattttaac ctcttccttt atctcttcca tggtaagttt
     3901 ttttctttta aatttgttgc atttactaaa accgcaataa acacattcat ttacacagta
     3961 gtttgataca taaagagggg caaataaaac cactctatta ccatatattt cattttttat
     4021 ttgcccagct atactaaata atctatctag ttgtttttta tcttctactt caagaagtgt
     4081 agctatatct ttataactaa gtttttccct tctatcagcc ttatcaagta ccttttcaat
     4141 atcatcagaa gttgaatttt ttgcatcttc taataaagaa ttaataagtt catggtttat
     4201 gaacatttac aaacgcctcc attcaatatt atcaccgcga gaatagtcaa cttcaagtcc
     4261 taatgacttg atgttttctt ctaaactttg gtgatattca caagcttctt gaccagtaaa
     4321 ggctttgtta tcgtaaagag aatattgttt tctaaattct tgaggagaaa ggtttggcat
     4381 aattacattg caaccagcta aaagacctgc atttctacca ctagggttta tagatgccaa
     4441 ggctgttgta gcgggtaata aaacttttgg aagtagaagt cttgtaattg aaagcataag
     4501 taatgtcttc tctaaatctc catgtttata atccttaaaa attgtatcat ggtgtggaat
     4561 aaaaggtcct attccaacca tatgaggatt taactctttt aggtataata aatcacgaac
     4621 aaggtcttcg ttgttctgaa atggtgattc aaccataaat ccagcaccta cctgataacc
     4681 aatttcttta aggtttttta ggcattcttt tctagttcta agctcaatgt ttggcggatg
     4741 taatttttta taatgactgt ctgtagcagt ttcatgtctt aaaagatatc tatctgcacc
     4801 acataaaaag tattttttat acgaatcata actcttttct cctagtgata gagttatggc
     4861 acaatcctca tatttatttt ttatacttga aacaatttca catattttat catcagtaaa
     4921 atatgcatct tcaccacctt gcaaaacgaa tgttctgtat cctattttat aacctatatc
     4981 acaacattca agtatttcat caagtgaaag cctgtatctt atgatatttt tattagaaga
     5041 ttgtattcca caatagtagc aattattttt acaatagttt gtaagctcta taagtcctct
     5101 caagtagact ctatttccat aatgtttatt tctcatgtcg ctagcttgtt tataaagata
     5161 gtttcttaaa ttttgttcga agttatattc ctcattgtcg atattttcta ttaaatatag
     5221 tagttcatca aaacttaatg aattaatgct gtaaagttta tctataaagt atttaatctt
     5281 atttttagtc atattcaaat atgtaattat taagtatagt taaattttta ataattgaaa
     5341 agttcaagtg atctttccaa aatacctttt acatatgcta ttaagacacc atagtttaca
     5401 ataggtacat tgaaagattt tgccttttct attctagata acattccagc tctattcatc
     5461 atacaagctc cacagtgaac tacaagggca tatttattta tatcttcagt gaaggatact
     5521 ccagaactaa attcaaaagt aatgtttttg ccagttttct ttctaatcat attaggaatt
     5581 tttactgttc caatatcatc agtttgtcta tgatgggtac atccttcggc catcaagatt
     5641 ttatcaccat cttttaaatt ttctaaagca taagcgccat taatcaattc tttaaggtct
     5701 cccttttgtc tagcaaacaa tatagaaaaa gatgtaagag gaatatcttt tggtgtatct
     5761 ttatctactt gtggaaatac ttgagagtca gtaataacaa gttttggttt tttgcctaaa
     5821 ttagatagag tttcttttaa actatcttct tttgtaacta tagaaattgc accactatcc
     5881 aaaatatctc ttataacttg ctgttgagga agtataagtc taccttttgg agctgcttta
     5941 tctattggaa caactaaaac aattaaatca tttggttcaa ttaaatctga taccaatttg
     6001 aactcagtgc tatccttagg caaaactttt attatttcat tttttaaatt ttcaattcca
     6061 attttatctg ttgatgagac agaaacaact ggacatttaa ctttattttt agttaagttt
     6121 aaaatttctg attgattctt aatagtatca attttgttga gaattagaat atgagggata
     6181 ttcttatcat taaacttttc aattagtgac aaatcttctt gtgatatacc aatctggcaa
     6241 tccactacta gtaaagctat gtctgtcttt tctaaaacat ctagtgattt acctattcta
     6301 agttcaccaa gttcaccaac atcatcaaga ccagcagtat ctataagaac acaaggtcct
     6361 ataggaagga tttccattgg tctaaatact gggtctgtag tagttccagc aatatctgag
     6421 actattgcag cactttgatt tgttatagca tttattatgc tagatttacc tgcatttctt
     6481 ttaccaaaga gacctatatg tactcttacc gattgtggag tagaatttag actcatttgt
     6541 actcaccacc ttaaacttct ttttttgata tactagtttt tacagataca tgaggtatgt
     6601 tgcctaattt accagtcaaa ctattaatag tatccatttc gccaacaact gctatacata
     6661 cgattgagac accttcttct tcgcaaggaa tgcccattct tcctttaata atacctttaa
     6721 aacctgatac aacctcattg aatttaaatt gacattcttt tggctcttcc aaaatagcac
     6781 ttataactgc aacctttttc ataaatatcc tcctaactca gaaaatatgt cttaaacaaa
     6841 tccaaaatca aaaaactttt ctttgtaaag aaaagtttct acaaaagaaa agttattaat
     6901 tacacaagta taatgtgcta tattaaacta tttccttaca gattagataa ctcttttctg
     6961 gtagggttag cttgtaaaaa tttaatttaa caacaagtaa ctgaagaaaa tttttataca
     7021 atttatataa attataataa aaattaacta aagaaaattg aagttactta tataatagta
     7081 gtaaattgaa attaagtcta tagaaaaacc aatttttgta taaaaataaa aattaaagtt
     7141 aattatttgt taagaattga ctttggtaaa atatataatt aattattctt taagcatata
     7201 atttataagt aattttagtg tattttcaat agcttctata tgtgttcttt cataatggtg
     7261 actattgttt gttccaggac caacacatgc tatttgaaca tcttctcccc aacgaatagc
     7321 ttctgaagca tctgaaccat agaaagtaaa tacatcgaca gcgtagtcta aattattgtc
     7381 ttctgcaaca tcgacaagct tgtttcttaa attaaaatca tataatgata aattatcttt
     7441 tgcaactata gttacaccat gttcactaga tgtttgactt ggtccagtag gtgctatatc
     7501 tattgctaca aattcttttg ttttttcagg taaacattta gaaacaccat gtccaatttc
     7561 ttcataatta cttatataaa agttagtagt atgcttaggt gttaaattgt tttctttaaa
     7621 atatctacat atttcaagaa ccattgctac agctgattta ttatctatat atctagattt
     7681 tataaaacca gattctgtag cctcaaatct atggtctagg catacaaaat ccccaacact
     7741 tattcctaaa tctaatacat cttgctttgt aaatactttt tcatcaagtc taataatcat
     7801 tgtttcttca tttctttcag tggcagcttt tgtttgacca tatatatgag tagaagcata
     7861 cttaaataca acacttcctc tgattttttt accttttcta gttataacag tacagttttc
     7921 tccctctata gctgcccaac atccaccacc aactttatgg tatttaagag taccatcaga
     7981 tgatatttct tttactatag ctccaagtgt atccatatgt gcagatattg ttatttgatt
     8041 tgaatcatct tcaccaggaa gagtagctat aagtgcacct ttttttgtaa gaatagtgtc
     8101 aagacctaaa ttttcaaaat cttttttgat ttctaaaact gcattttcag tatatccagc
     8161 tgggcttgga atgtccaaat attttttcat taaatttata gtattagtta aatttaaatt
     8221 catattgttt cctcctaaat aaatatattt atacaaatta taacataaat acaaatgttc
     8281 tgaaaactaa aattaaaaga atagaatcta aacatattta aaaaaaaagt atataaaata
     8341 aatttgattt atattatata gatttaaagt aaaaatagtt ttatttaata attaataaaa
     8401 ataaatacaa aacaaaataa ataaataata aaatatttag tttaattatc tttacaaata
     8461 aaaattttag taataaaata attgttgaaa aagattttaa attcagatta ttcatttcta
     8521 aagtaattaa tgtgcaaatc tttattaata tatgtcaata gttaggtata tatgtattta
     8581 attagcatga tatagataaa agtatttatt ttaattgtct attatctgaa aaagtcttta
     8641 cttagaaatc ataaaaatca aattaattag gaaaagaaag ggtgtgtata atgtgattga
     8701 gactggattt gtatattgta gtttcttggt tgtattcgta ggtgtaattt tttttatggt
     8761 tgataaattg aaatggaagg tatttgatgt tattaatcca atgcttttaa tatacttagg
     8821 ttcagctata cttggatcat ttggtttatt tgcacagaat gaagatgttg gaacgtatca
     8881 aaacataata acagtgaact tccttccatt aatgttagtg tttttgttaa ttcaatgtga
     8941 tttaagaaaa atattaaaga tggggcctaa aatgttatta tcatttttct gtgctacatt
     9001 tacatttatg cttggatttg ttatagcatt cataattttt aaaaattcaa tgggtgcaga
     9061 ttcatggaaa attcttgggg cagcatctgg ttcatggata ggtggctcat caaatatgat
     9121 tgcagcagcg aatgctctag gagttgcaga agaaggactt agttatgcaa tacttatggg
     9181 ttctatagga tatacagtat ggatgtctgt ttgtctaatg tctgtgaaat ttggacctaa
     9241 atttaacaaa tggacaaagt gcgatacaag ttttatagat gaggttacat caaaacttga
     9301 aaaagaaaat aaagataata cggcacctac gtttgtagaa cttatgattt tactttctgt
     9361 tgggtttgga gttgcgtctt ttgcacaaat tgtatccaag atacttccaa cttcaggtat
     9421 acttaatgaa actatgtggg tagtacttgt tgcatctaca gtatcaatat tattgggtat
     9481 gacaaaaata tcaaagctta aagggagtac actggtagga aatgtgttta tgtatttact
     9541 ccttgcagct acaggtagta aagcaaattt ctttggattg tcagaagcac ctgtttacat
     9601 aatgtttgga ttggtagtag tccttgtaca tgctatttca tttatagggc ttgctaaact
     9661 atttaaactt gatttattta cttgtgaagt aggaagtata gctaatatag gaggagtaac
     9721 tacagctcca atgattgctg gggttcacaa tcctgtatta ataccagtag gtgtacttat
     9781 ggggcttcta ggaaatatta tagggactta ttgtgctctt atggtaactc aaatattaca
     9841 tatgttagct taataaaaat atggtataaa attaataatt taaataagaa aagtcatttc
     9901 aatttaagca gaagctttta ctgaaatggc ttttttgttt gacaagataa tctatttcaa
     9961 gaagatgttt tgacatattt agtgtataat attgtaaaat ttactaaaaa cgagatatat
    10021 ttaattttta tattgaatcg aacgtctgta tgatgtataa taaatataca gaacgaatgt
    10081 ttgcagaacg ggtgtttttt gaatttattt taataattta tgaatatata tcagaatgca
    10141 taaatatatt taaatataaa gattaaaaag aatttaaagg agtgttgata acttatgata
    10201 attccatcaa gaagaataca agatataaca ttaacaaatt taaaaaatgg tgaagtaact
    10261 ttaattgaat tggatgaaat atacaagaag atgggatttt tatttgttgt tagtgaaggc
    10321 aaattgaaaa agataaaaaa ggagaatcat cattagaaat gaatgaaata ataaatatta
    10381 taatagttta taaaacctaa cttatgtaga atataaatac ttaagttagg tttttttgtg
    10441 ataaagttta taagtactca attttataag ataaataatg gtcttaagtg aaaagtaaat
    10501 aagtttaaat tttagtaagt attatattgc ataagaggtg atattattat gaaaatagta
    10561 acctacaata ttcataaagg tatggattca aataatagat taactctaac taaaatgggg
    10621 ttatatttaa aacaattgga ttgtgatgta atttgtttgc aagaagtatt atatcctcaa
    10681 tttttagctt taaaagctgt tttaaatatg gatggtgtct ttgctacaaa tgtaaaaaaa
    10741 gtaaatatga tatatggtat ttgtacattt acaacatcta aaatgttaaa cagtaatcat
    10801 tttttcttaa ctagcaaaaa agaacaacgg ggagctcttt gtataactat agatgcatat
    10861 ggaagaatta taaatgtaat aaatactcac ttgggacttg ataggcagga gagagcaaaa
    10921 cagttagacg aaataataga ctataggaac agattagtgg ggatagttat tttatgtggt
    10981 gattttaatg agaaaaatgt ctttttaagt atgtttaatg atatggctat ttcactaaac
    11041 aagtcttact taccaacatt tgaaaaatca aactctagaa tagattacat ttttgttaat
    11101 aaaaatactg aactaaaaga atacacagta gaa
//
LOCUS       DAGSHT010000112        11076 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGSHT010000112 DAGSHT010000000
VERSION     DAGSHT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.114"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..787)
                     /locus_tag="MJB72_002483"
     CDS             complement(<1..787)
                     /locus_tag="MJB72_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBY8263569.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKN"
     gene            complement(1154..3034)
                     /locus_tag="MJB72_002484"
     CDS             complement(1154..3034)
                     /locus_tag="MJB72_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBY8263570.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANAICRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNHKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYYIVSKETQESGSNVILKLGLAQKTIKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVDKIVNKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(3244..9207)
                     /locus_tag="MJB72_002485"
     CDS             complement(3244..9207)
                     /locus_tag="MJB72_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBY8263571.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLSVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKILESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDNNSTTSTYSKSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKILTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSVQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKRNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTIDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(9331..9416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(9588..10991)
                     /locus_tag="MJB72_002486"
     CDS             complement(9588..10991)
                     /locus_tag="MJB72_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBY8263572.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALTASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         3028 a         2012 c         1268 g         4768 t
ORIGIN      
        1 agtttttagt aagtggtgca gctgctattg catctactaa ttggtatcca tctgatacat
       61 agaatccatc tgcactatta aaaaagtagt ctattacgtt tttgttagtt tcaaatcggt
      121 cagttccttc tattcttact gagtttgtat ttttaactag aggattactc attatctcct
      181 ctgaacctat acaatatgat tgaatatttt ttacatcaaa aggcacactc tttccatctg
      241 taagtatgat tggagctcca tctctagtag ctactgatga tacactcatt gcatctgctt
      301 ctccactata tgcattagtt agtagtactt tatcaacttt ttttatagtt gctatttctt
      361 tagctattaa atagcttgtt tttagtctat cttctccacc aattcttttg acttctatat
      421 ttttagaatc tagttctttt tctactgatt tacttatagt atcttctgta cctattatat
      481 atgctttttt gatatttttt agacatctat ttgtgtctgc tggtatctta ttttgttgtg
      541 taaatagtat tggagctttt gtagctcctg ataatccact tgcacttaat ccatctgcaa
      601 gtgatttttc tgtatttact aaaattactg tttcataagt ctgtttatca gctatctttg
      661 ctgctgtttc atatctatct ttaccgtata ttttttctac tactgatgat gcatttacac
      721 ttttgaagtt tactaagatt aatgatactg ctagacctaa tgataatatt tttttgttca
      781 ttttcatatc cccctgatga tttttatatt ttatatttat attgtgatta ttttccattt
      841 attttctctt tattttccat atattataga gtatatatga tatgaatata tctcgtctat
      901 atattagtgt tttattttat taaatttaaa aaattatttt taacttcttt ttaaacatat
      961 tcaaaattga atttatattt atatatataa aagatatctg ctttatttaa aattaaactt
     1021 aatatagctc aaattattat acattttaac ctcaatatta ttagattgtt aagataatga
     1081 aaataaaata agaatgcatt taaatatata actttatatc taaatacatt cttattttct
     1141 atttacttaa tatttatttt tcaattaaac tcttaatctt atctattaca gatgaggcta
     1201 ttcctccacc tacttcaaac aactttgatg aatttttacc ttttaatgac gattcttgtg
     1261 atttatctaa cgtatttgta gcaagtataa ctggtgaatt gtttttagct gctaatggtc
     1321 caactgctaa agcgtctata agtttatctt gatttgcaac tacaactcca tctagtttag
     1381 attgagagta gaatttatct attactttgg cattcgtctc ttgtctagtt tgacctgcca
     1441 ccctattgtt tgatatatct ttgtttacta ttttgtcaac ttgttcaata gccttgttag
     1501 atattttttc tattccacca ataaaatata tatttgacac cttatttgat ttaataaatt
     1561 gttcagcttc attatctaca ccatcttttt gtgtaagtac tattggagtt ttttctttac
     1621 cagccagtga tgctattgat aatgaatcag cttctccatt tcctgctcct atatacaact
     1681 tgcttacatt atttgtctta tctatttctt ttgctatatt aatagaagtt tgatatcttg
     1741 tatctccacc tattctttgt actgatattt ttgaatttac agctttgata gcttcaacaa
     1801 ctgaatttgg cattgagttg tttccaccta tcacaattac ttttgatgga tttagccttt
     1861 tcaattcttc tacagttttt tgaggaagct tttcatttga tgataaaaga attggtgagt
     1921 tttttacact agcaagtggt gtagctgtaa gcccatccac catactttta tcttctccat
     1981 ttactattac aactgtatct gattttgtcc aaccactttg agatattttg atagctgtct
     2041 caaatctatc agcacctttt aattttttga ctgtgacatt agatgtagaa tcacttgaat
     2101 ttcctcctcc agagcttcca ccactagatg agccgcctga attacttcct ccgccattat
     2161 ttccaccatt gccattatct ggtggtaatt catctggttt tgataattca ttttcaacta
     2221 tgtattctaa actttttgga agtgtaactt caacatattt gtttattgta gcttttcttc
     2281 ccttaattgt tttttgtgct aatccaagtt ttaatattac atttgaacca ctttcttgtg
     2341 tttctttact aacaatataa tagtttatcc taatattgtt taataaatat tcaagtgctt
     2401 cacttttctc taccttatac ttttcagggt catttttact agaaccagtt ccaccaattg
     2461 gttttacttc actctcatta ataagggtaa atattaatcc ttcatcagag cttatatttt
     2521 cataatccac tgttttgttg ttatatgtta caatttttaa agctggttta tggtttgttc
     2581 cttttcctaa aactttatag tcagcatttg agttactgcc agattttttt acagatattc
     2641 ttcctatatt caatctattt ccgtctagta agtcttcatt tgatgtaacg tatatatcta
     2701 atgtagttgt attacttgat ttgttgtatt ctgctctaca aatagcattt gcatttccac
     2761 tttttatctg agattttata ttatcagacc aagtaatctt ttctttctgc tcattatcta
     2821 ctacttcaac ctcaccttgt acttttatac tggcttgaaa tgaatatact atgttttcta
     2881 ctgagtccac agatatatct atagtattta ctttctcata cgtttttgtt ccttgtaaaa
     2941 ctggagctgc atatgcacat gtagcactac cagttacagc tactgccata gttgatactg
     3001 ctatagttct tataaaagat tttcttttac tcattaacac ctgtccttat ctatcattaa
     3061 ctaagtggat aaatttactt atccacctag cttttatatt taatttataa attaccctaa
     3121 atttatggtt acatttatat actttacttt taatatctac tattttaaaa ctattctagt
     3181 acctaatctt attagatata caatgttagt gttagtattt ttacgttttt agagttccac
     3241 ctactattta tctctaacaa tattaccctt tactttattg cttacagtta taaccttctt
     3301 attactgata tcaatagaat ttcttgtagc ttttctagct ttactattac tatcagtaag
     3361 acttattgtc tttaactcat cataattctt agctcctaca ggtacataca ttgtatcact
     3421 tacaaatctt tgacctactg gagtatctcc ttgaaggtca ttgtatctat atagttcagc
     3481 cttaacctta tcacttaaag aatcaaagtt ttcttttggc atatagtaaa cccatgtacc
     3541 tttactgatt tcacctagtt cacctttttc tggaacttct gcaaataaaa gtgcttctcc
     3601 agatatagtc ttattgtttt tatctataag tagtggtaaa ttaaatgctg gatttccact
     3661 gtattcacct gtaataacta ttgaacctgc tggaatctta cctgtttcat cttcacttgt
     3721 gctagagtca tcactttgat atacataatc ttctgctaat tttccaattc cattagtacg
     3781 agtttggtct gcattattca ttgtgccaat ttctatatta tcacctggag gtcctattat
     3841 atctaattct gcaacttcta tattagactt attatttgag attagtttta catatctagc
     3901 ttggtatgtc caaagtttac catctttatt aaatgtaact ctagcttcat tatcttggtc
     3961 ttttcctcca ccaactaaat cctgtccata ttcaaaagtt ccttgactta catcttccca
     4021 gttcttttta tcattactta cttgtatctt gtaattagtt aaaggattgt atgttgtgct
     4081 agtattcttt ctaaagttaa ataatccttt taatgagaac ttcctagctt ctgcctctgg
     4141 ttttgtatat tttagtccta ctataggtaa tgtttctttc atgtctacta ttatatatgg
     4201 attttcttct acatttatat cagggtcttt ttgagggtct ttgttccact ctgctttact
     4261 tattatcttt cctttatatg tagtacttgc atcattatct tttatattat caatttcagg
     4321 attttgtaat ggtccatgtg agtcattttc agttctaaca tcaccgtcag aaacagtatt
     4381 tgttatgaat gaccatgctg atttatttat tttcccttca tctgataatt ttacagtgcc
     4441 aacttcaaat tcttctgttg gatttaggtg ataatcatat gcaactacct tatatgtaaa
     4501 tgctctgtta ttaagaggag atatattatc agtaaattta gccttactat ttccttttgc
     4561 atccacaaat ccaactgttc tgtattttac ctgtgttcca ttttcaccat ctaaataatt
     4621 tccatcactt cttattattt cgtatcctag tatcttatct ttatccttat ctacatctag
     4681 gtctatagat attttattag atttaactag agaaccactt tgtattccat cagaaaaact
     4741 agcacttact ttagtatctt ttgccatact agctatattt tcatttaaaa tctttcttct
     4801 agcttcgtca tttacatatt gtatctttct tgattctttt tcgtagttct tactatccat
     4861 tatactgttt aattctggtg taatttctac tccccatgcc ttaaagtaat ctcttaaatc
     4921 tttttcaaca acacttgatg ccattgctac tagcatttgg tcttttggta aactattaat
     4981 tgggtcacta cttgaaagtg ctctgtattt tcttgaaatc tttgcataga atgtatcatt
     5041 acttaaatca gcatctgagt tattctttaa cattgaaccc gtaaaatcat catcataagc
     5101 tagatgtaat tgccataaca ttccaagcct ttcaaataca ttgcttggta tacctaagct
     5161 atttgaagtt acatgctcat atatcttatc cattgttgaa ccttcaagtc ttgaatgtgt
     5221 cttatcatca aaggtctgag caagtaaagc aagtacattg ttactagttt cactatatgt
     5281 catctttcca atatctgtaa catgtcctat ctcatgacct attccccatc caaataagtt
     5341 tcctcctgta gcttttccac tatcatctat ttggaaaggt actccattca taagccctgg
     5401 aactgaacca tattctactc ctacatgtaa tccagaagca tacataaatg ctccaataaa
     5461 cattctttgg tattttatgt tcatacgaga ttttggagcc atatcatttt tagttaagtt
     5521 aaattcatta tcatctatag ttccattacc atctaagtct tgtgttttaa atactcctct
     5581 ttctgagtaa actaaatcca ttatttgttc ccatgcaagc atactattat ataatctatc
     5641 tacttgtgta tttaaatcag catttctttt tgatatccct tgatatacag ctgttgcagg
     5701 aacatttaat gttactttat cactttcaat atctgtagtg tttaacacac cagttttttc
     5761 tgaatatcca tatgaattac caacatttgt tcctggataa tatggttctc cttttacagc
     5821 atctttatat ttagctaatt cttctatata tgcttttatc tttttcttag attcagaaac
     5881 tcccgcagga ctcttatcat ttattgttcc atttagatta agatgtggta tcttagtagc
     5941 tcctgctaca cgtactttta tatctcctcc acttggagta tcttcagcgt atcttatgta
     6001 taaacttcct ccacattcta tatctaaact atgaagtttg cttaattgaa ttacattttt
     6061 tccagtgtta agatttattt ctccactctt atatgcacca gattctccat agtattgtgt
     6121 atatatcaac ttaggagttt ttccactttt acttccaaca tatactgtta tttcatctcc
     6181 tgctctagca gctactccta atgcctgcca gtcattttgc atacctaaat tattggaatt
     6241 tacagtatac tgtccgtttt tatctttata ttttcctgat ttatttattt cttgatttac
     6301 agtagttaca tcagtaagtg tggctctatc attatataag tcttgagcta attttaattc
     6361 tctatcaata actgttttat atggatgata ttctccacta gctttatctg gagtatttaa
     6421 tctatctttt aattcggtta ttttttcttg agttacatct tcttttaatt caacttgtag
     6481 gtcatccata aatagatttc ttacatcatc ttctatactg tcatactcat aaaacttaag
     6541 ttctgatata ttagacttat atgaataaga tgaacaaaca cttacttgta ttttagtagc
     6601 agttattggt tcttttaatc taaccattac atacttatta ttatttacag ggtctgtctt
     6661 tgtttcaaaa ggaacatctc ccaaattgtg taattgacca ttttcatccc aatatcgtac
     6721 ctcaaagtca tttattgtct tatcagaaac atcaaaacga ggtgtagcca taaaagtatc
     6781 catagtataa gatttatcaa aagttattat tggtcctcta ttcgttccct cagctgcatt
     6841 atatgttggg aatgtccaat gtgttgtaaa gtccccatct actacattga atttatcaaa
     6901 tggattttta tcactatatt cacttttgtc atatcctgct ggataagtaa catctacaat
     6961 gtgatttgtt ggtattccaa atccaccatt ctctttagga gtattaatta atctatagtt
     7021 aggtgttgtt ggtgcatcca tgctttcagt tgtacctata tatgttttag acattccact
     7081 agttcccaag tggttagtag ctgtcattct aacttcatat gttgtattat ccttaagacc
     7141 ttctatcttt gaacttgtcc ctcgaataag cttatcattg tattttatcc aagacccatt
     7201 agagcctttt tctttatagt acaagtcaaa atctttagct tgtttatgat ttttccagct
     7261 tacatcaaga gatttaaatt tacctttaac atttatacct tctggtggtt ctggttttgt
     7321 gtctggaaca acttgaaatt ctaatatact atctgggcta aattgtatag gtttatagtt
     7381 tgaatctaca ttttctggta ctccatctaa gttttctggt gttgcatctt tataaccact
     7441 agaccaatct ccatttaaag attgtacgct aactctatat agtccatatg cttctatatc
     7501 tctaacttct attgtatttt tactagtttg taatttttta gtagataata cttttccctt
     7561 actatcaatt ttgtctagta taagttcgta tgctgataca tttacttcat tattccaata
     7621 aaaagatact ttttcatttc caacagcagt gctagtcaat attttattta ttttaggtat
     7681 attcatctct ggctttggta tttctttata tacattgttt aaaaattcaa cttttgatat
     7741 ttctgcaaga tttctatcat ttgctgttgt agctgttatt tttatagtta tcttttttac
     7801 tgctacttgt ttaccaagat ttattactat atcactattt tgaacatctg catatgtttc
     7861 taatgctgac ttactatatg tagatgttgt agagttatta tctttactga tattaccctc
     7921 ttcttttgga gtatcttcat ctgaattagg tttatccatt gatgaatttt ctttatcatt
     7981 atttttatct tgattggtat tagagtttgc ttcttgttta tcgctatttt cctgttgttt
     8041 atcattattt tcatctttat tatttgagtt attatcagtc tggtttatat tttcatcttt
     8101 attatttact tcttcttgat ttacttcttc ttgttcgtta tttacttcct cttgttgctc
     8161 ctcatctttt aagtttttca tatattcttt gttttcataa ggaatctgtt gaacattacc
     8221 atctttgtca gtaacctcaa ctattccttt tgctggagca gtaccttctg gagcttttat
     8281 attgatgact tccatattaa gtgcttctga taaagctata tccattactg ctggtttttc
     8341 ctcagatatt tcatcatttt ttgaagatat tgtaactcca ttattttctc cactaaataa
     8401 attttctaca ttaccattaa tatctacatc actatttcct tttaattcaa ccttacttgt
     8461 atccactata ggattggtag atactatact ttctaggatt tttgcatctt cactttgtcc
     8521 taaatttttc tgtacataat ctaagtcaac aatatctacc tttccatctc tatttaaatc
     8581 aaattctctt ttatttgaat ctatattctc taaaacctta ttataatcac tcttatctac
     8641 tttaccgtct ccatttacat cacccattaa gaataattca taatttaaag gtttattttt
     8701 attatctaca cttgctctat tgcttaataa gactctttgt gaataacttg atatatcaat
     8761 gtctttaatt tctttgttct taaatccagc tccagagact tctacattgt attttccaac
     8821 agataaatta ttaaatacaa tattataata tgatatattc tcatctttat tttttatttc
     8881 acttctactt gcagataatt tttttatagt atattctata ttatctgtcc caacatttac
     8941 tgttcctgaa tctttttctc caagctttat tattccacta ttgttactat catcttttaa
     9001 tcgtaactgc atattagtca tactttctga ctcgctattt tttattggca gtgaaaaatt
     9061 taaatctact tcaagactcc cccttaactt ttctgtaact gatttttcaa ctgcccttag
     9121 cttcccctct aatataggtt ctgcaaaaac gtttagtggt gcactacaca tagatactgc
     9181 tagtacacaa gatattgcct ttttcatgaa ataacaatcc tcccttaata aatatctaat
     9241 ttttcatgaa tttaaatact ataagtttca gacaataaaa aaagacactc aaagagaatc
     9301 ttaattataa aataaaaaga ctttactctt tttagcaact ggctgatatg gctttaaaag
     9361 ctttaatccc tatagttttg cgtcattaga tttccctaat tttgccattt aattttcact
     9421 actttcttac ccattttata ctatatttaa actttttaat acaatttttt tagattaaat
     9481 tataaaaaaa taacatttat attaatttat taatataaat gttattttcc attataattt
     9541 aattatttat atagcttcct tacaagcata attttgtgta tattttctta ttctttatat
     9601 cttccataaa attcatgtgt agcatataga ttcttatctc ctgttatagc tataccaaat
     9661 cccattattt catacccttt atgcaacata ttagttcctt cttctggatt tgtcttccac
     9721 tcattaaaaa taaagtttcc tatatcatct gcatctctat ttgtatatac aggtttatct
     9781 ggaattttat atttaattat attttcacct tgaactgcta tatatccagg tttaatctca
     9841 ctccaatcta agtatttcaa aaaagtagaa tatgaatttt ttccatttat cgtatgagat
     9901 aaagtcttct tatttgccat taaattagac caatcatttg ctagaccctc taatctgctt
     9961 gctacagata acgctcttac tccattctct tttctatact gctttgtaag agcatatact
    10021 ttacccttta ttctctcttt aaatgctcta tcatttggat tgtaatataa accttgttga
    10081 tttggtgaag cttgatttat acattgcttg ataacattct gatttatgcc tcctagtaca
    10141 gtcaatttgt ctgcaccact taaaatactc ttattactgc cattttcaac taaaactaca
    10201 ggagcatttt ttgctattgt agaggcagtc aaagcgtctg ttaattgtaa gcctttactt
    10261 agataaaatt ctttactatt tttataaaac tcttgtataa ctatcttatt agtttcaaat
    10321 ctatctgtgc cacccaatct aatagaattt gtgctcttaa ctaattcgtc gctgattatt
    10381 tcactagaac caagcgcata actctttaaa cctgtcgtgt caaatcccac actatttcca
    10441 tctgtaagta ttataggtac tccatctcta gcagctactg gtgatgcact tatcgcatct
    10501 gcttctcctg taaatccatt ggctataatt actttatcta ttggcttaat ttctttgatt
    10561 ttttcagcta tttttatact tgtatcaaat ctattttcac caaatattct ttgtacaacc
    10621 ttctttttgg atagtaactg attctcaaca tttttactta ttgttgattc tgaacctatt
    10681 atatacacag tctttgcttt gtttaatctt tccatagttg tgtttggaat ctggttttgc
    10741 tttgtcatca aaataggcgc atttaaggct ccagctaaac cacttgcact tagaccatct
    10801 gctaagctat tatctgagtt gattagtatt gctgtattgt agttttgctt atctgctatt
    10861 tttgctgctg tctcatatct gtcattccct tttatttgct caactgatgt caacgcattt
    10921 accataggag tatttaccat tactaaaaat agtgtaagtc ctatagctaa tatcttttta
    10981 ctattcttca tattattttc tacccccaat gtacttttca tattttttat tagtactcat
    11041 acttattttt aatcaaaagt tacaattttg ttacca
//
LOCUS       DAGSHT010000113        10723 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGSHT010000113 DAGSHT010000000
VERSION     DAGSHT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.116"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..268
                     /locus_tag="MJB72_002487"
     CDS             <1..268
                     /locus_tag="MJB72_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY8263573.1"
                     /translation="GKIHDDYRIDYLRSHINACKLAIEDGVEMIGYCPWSFTDLLSSS
                     QGFNKRYGLVYINRTDHEVLDLKRIKKDSFYWYKEVIENNGIVK"
     gene            296..1144
                     /locus_tag="MJB72_002488"
     CDS             296..1144
                     /locus_tag="MJB72_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8263574.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            1805..2497
                     /locus_tag="MJB72_002489"
     CDS             1805..2497
                     /locus_tag="MJB72_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263575.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLAELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            2494..3429
                     /locus_tag="MJB72_002490"
     CDS             2494..3429
                     /locus_tag="MJB72_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263576.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            3577..4500
                     /locus_tag="MJB72_002491"
     CDS             3577..4500
                     /locus_tag="MJB72_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263577.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            4484..5254
                     /locus_tag="MJB72_002492"
     CDS             4484..5254
                     /locus_tag="MJB72_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263578.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            5264..6046
                     /locus_tag="MJB72_002493"
     CDS             5264..6046
                     /locus_tag="MJB72_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263579.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            6422..6958
                     /locus_tag="MJB72_002494"
     CDS             6422..6958
                     /locus_tag="MJB72_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY8263580.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            6990..7379
                     /locus_tag="MJB72_002495"
     CDS             6990..7379
                     /locus_tag="MJB72_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY8263581.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            7416..7802
                     /locus_tag="MJB72_002496"
     CDS             7416..7802
                     /locus_tag="MJB72_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBY8263582.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            7929..9371
                     /locus_tag="MJB72_002497"
     CDS             7929..9371
                     /locus_tag="MJB72_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY8263583.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            9668..10483
                     /locus_tag="MJB72_002498"
     CDS             9668..10483
                     /locus_tag="MJB72_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8263584.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTIELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCRDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         3831 a         1344 c         2008 g         3540 t
ORIGIN      
        1 tggaaaaatt catgatgact atcgtataga ctatttaaga agtcatataa atgcatgtaa
       61 actagctatt gaagatggtg tagagatgat tggatattgt ccatggtcat ttacagacct
      121 tttaagttct tctcaaggtt ttaataagcg ttatggactt gtttatataa atagaactga
      181 ccatgaagtt ttagatttaa aaagaatcaa gaaagatagt ttttactggt ataaagaagt
      241 tatagagaac aatggaatag taaaataaac ataaatatat attgagggtt ttagtatgta
      301 cattacaaag gtattaaata attcgttgct acttgcaaag gatgataatg atgaagaaat
      361 aatattgatg gggaaaggca ttggccataa ttataaggcc gggtctgagt taaataaaaa
      421 agatatagaa aagatttttg ttcttcatga tgaaaatata aaaaagagca ttatacaatt
      481 ggcaacagaa atagatgcag aatattttgg tatagctcaa atgataattg catatggaat
      541 agaaaaatat aacttaaaac ttatgaacca tatatatttg gccttaactg accacattgc
      601 ctttgcagtg agacgtttta aagaaggtat aatgattgaa aatcactatt tgttcgaaat
      661 caaggaattt aatccaaaag agtatgatat aggaaaatat gctattactg tttttaagga
      721 ggttcttggt ttagacctac cagaagaaga gattggtaat atagcaggcc attttataaa
      781 tgcacaacaa gataatcctt atagtgatag aaataagaga agtgctaaaa ttgtaaatgc
      841 tattttgcaa atagttcatt atcacttttc tattgtatat aataaagagt cattttatta
      901 taggagattt gtaatgcatc taaaggcctt ttcacaaaga tttctggcta atgaaccatc
      961 aagagaaaaa atagatttta tatatgagca agttcaaaaa aattgtaagg aagagtatga
     1021 atgtgtgaaa aaggtaggag cttatatgta taaggaatac tccagagaat taccaagaca
     1081 agaggagtta tatttgatga ttcatattca taagatttta ggagaattat gcgaaaatag
     1141 ttagaatatc ttaatatgct agtaatatta taaaaaatta aaaattaact acactttaaa
     1201 tatgaataat tgcattactt aagggggttg tcttaaaatt gattttttta attaaatttg
     1261 aagctcaact ccttttttaa acctttatat cattacaact ttaatgttta tacgcctgta
     1321 cttcagatgt atatctactt tttacatttc tcaacatgca aatctaccat tttttgcagt
     1381 atgttaattg aaataaacca ttgagttaac cctaaattta ttctggactt ataggaatag
     1441 ttccaaagat actttaaaat attttctgga ataattgctt atttcacata tgttttattg
     1501 tgatgttttg atttaataaa ttcttcaact ctgtatatag taaatgtatt atcatgtttg
     1561 ttttattcaa gcacacttat aatacgcttg attttttcac aatgatgaaa tatactaatg
     1621 ggtgttataa gtataagaat agcattacac ttgaagaagc aagtgataaa tgaggtataa
     1681 atcctcatat gataaactac tatttcattg aggagcatat tctaaatgat gtaaagatac
     1741 tacaatatga ctaaattcct aatgataaaa aagtctacga atgagtgtag aaaggggaac
     1801 taagatgaat aaagttttaa ttatagatga tgataaagat ctttgtattt tgttaaaaaa
     1861 gagtatctca attgaacaga ttgaagcaaa ctattgccat tctgggagag atggactggc
     1921 tgaactttca aaacaagatt atcagttagt agttctagac gtgatgatgc caggtatgga
     1981 tggttttgaa actatggaac aaatacgaaa atatagcagt attcccatct taatgctgac
     2041 atcaaaaagc gataattaca gcaaagtgca tggattgcga gctggtgcag atgactatct
     2101 aaccaaacca tttgaaatta cagaatttac tgctcgtgta ctgtctttaa tccgacgtta
     2161 cacccgtttt aatccaaaaa atgaggagcc acagcaactt tctttcaaag gtttgttgat
     2221 tgaccttgac cagcatacag tgactaccaa tggcgaacct atgttgcttc cacccaagga
     2281 atttggtgtg cttctttact gtgcaaagca tcagggtaag attttgacaa aacagcagat
     2341 ttatgaagcc gtgtggggtg agccatatgt atatgatgac agcaatatca tggctattat
     2401 cagtcgccta agaaaaaaaa ttgaactaga cccatcatct cccatctatt tgcaaacaat
     2461 aaaaggaatt gggtatcaat tcaacacgga ggtatgaaaa tggacgaaca aacgataatt
     2521 cttttcttac tcttgctgtc gattggtcta ctttttcttt ccatatttgc tttttccaag
     2581 cttgggcata tttacaagcg cctaaaagat atagaggaga tactggctga tgtacaaatt
     2641 ggagacgaaa atcgtaaaat actaattaag ccatgtgatg ttatggctcc attggtttat
     2701 cagcttaatg agattgtcta cgattatgaa aataagttac tttctctgaa aaagtcggat
     2761 aaagctagca aacaacttat gacaagttta tctcatgatg ttcgtacacc attaacaact
     2821 ctgattggtt atctggatgc tgtacacagt ggtatagtta tcgaacaaga acgagaggaa
     2881 taccttgaaa tcgctaggcg tagagcctac gacctcaagg attacattga tgttcttttt
     2941 gactggtttc ggttgaactc tgatgaattt acgctgtcaa tagaatctgt agaaattgca
     3001 gagttgtcac gcaatatttt gaaagactgg attcctatat tccatgaaaa gaaactgaat
     3061 tttgaaattg atattccaga aaatcgacta atggtaaacc tcgacccaga tggatattcc
     3121 cgtgtggtca ataatctggt tcaaaatgtt cttgctcaca gtaaggctcg gcaaatcaag
     3181 attaccatga gtgaggattc cagaatggta ttactccgtg ttgaggatga tggtgttggg
     3241 attgccagag aaaatttacc acatattttt gagcgactgt ataagtgtga taaaggacgt
     3301 tcagaaaaag gaagtggtct tggtctatct attgtcagtc aaatggtgga gcgaatgggt
     3361 ggtcagattt ccgtagaaag tgagattgga aagtatacca tatttacagt gagcttacca
     3421 ttaatataaa attgtttttc ccccatgtga catgtataca cagggggatt ttttgtaagt
     3481 tataaagttg caaggttaat acaaggttgt tgcaaggtta atgtaagatt gaggtggtat
     3541 gataacttta ataaacaaag gaggctttta ttatttatga ataatttaat tattgaaaca
     3601 aaaaatctca ctaagcaata tggtgctcaa aagagcgttt ctaatttgaa tattcacgtt
     3661 cagaaaggtc gcatttacgg attgctagga agaaacggtg cgggtaagac gacaactatg
     3721 aaaatgttat tgggtctaac aaaaccaact actggagaaa ttaaaatatt tggtcaggac
     3781 attcacaata acgagaagaa gcttcttcct cgaattggat gtttgattga atcaccaggg
     3841 ttttatccta atctgacagg aacagaaaat ctcaaaatct ttgcaaggct tagaggaata
     3901 ccaaaaaaaa atgcagtaga atcagcactg gatgtagtcg gtttgccaaa caatgacaaa
     3961 aagttatttt ctcaatactc tcttggtatg aaacagaggt tggcgattgc tcttgccatt
     4021 atgcatgacc cacaactgtt gattttggac gagcctatca atgggcttga tcctattggt
     4081 attgcagaag taagaacatt tattcgtgat ctctgcacag agcgaggtaa aactattttg
     4141 atttctagtc acattctttc tgaaattgcc ttgttggcag atgacattgg cattattgac
     4201 catggtattt tgttggagga agaaagtctt gcccaattag aagaaaaaaa cagtaaatac
     4261 atacattttg ctgtatctga tgcagcacag gcagcaaggc ttctggaaac ccaacttaac
     4321 ctgaaaaatt tcaaagtagc agataattac aatattatgc tatatgatac agatgtttct
     4381 gttgcagaaa taaatcgtgc tttcattcta aacaacattg aggtatcaga atctcattta
     4441 tgcgaagata ctcttgaaga ttacttcaag aaagtaacag ggggtgaagg aattgcttga
     4501 tataatttca tgtgaatttt caaaactcaa gcggttaaaa tttatactga tttctatttt
     4561 aggtgcttgc ttatttccaa ttcccgcaac aattttgatt gcaaaggata acttgccctt
     4621 tgagcaattg ttcaaattag ttgtcaattt tggatatttt ttgctattac caattgtatt
     4681 aagtattgtt gctagccaac tattttttat agaaagagat aatgatgtat taaaaaatct
     4741 tgcaacagtg ccagtgcaaa aaggaaaact ggctctggca aaattggctg tgttgctgtt
     4801 tatatcattg ttctactctg ttgcagggtt aggagctact atcattggag gtttcattgt
     4861 tggaatagta gagggggttg ctgtaaaatt aggaatgagc attgcttttg gaattatgtt
     4921 ttttgttact gtacttcctg ttgtggtact cattgtatat ttcaatcgca gctatatttt
     4981 ttctattata ctttcttttg tatatgctat tttcaatttt agtatatcct taaatattat
     5041 caattttgaa ccaaataacc caatattaag tgtattacct gctcctgtta taatgcgttg
     5101 gtggatggca ttttggggag atcccactgg cgaatacacg gcacttagac agccatattt
     5161 gctctcaact cctgcttgtg taggtatttt atgtttgata accattatag cagtcctgct
     5221 gatttgtatg atatacaaaa agcaggagaa ttaggaggta tctatgcttg atataattac
     5281 tacagaattt caaaaaataa aaagatataa tattctatgg attggtgttg ttgctgttct
     5341 gttttcagca ctattggcag tttttcaggt tgtaagttca cacggcagtg accctctaac
     5401 gtatgagagc tttgcaaacg gagttatctg gaataatttc agtcttagtt ttccatttgg
     5461 gattacactt attggaggat atcttattaa ccgtgaatat acagaccaaa ccttaaaaaa
     5521 catcctaact gttcctattt cattacgcaa attgctagtg ggaaaattaa ttgcggttgg
     5581 aggaattact gtattgttaa gtcttttcag cttcttatgc acatttattt tggggttgtt
     5641 attttgcaat attaatatat ctgttacact cgttgtaaaa tccttagttc agattttgtc
     5701 tgttaatctg tactgttatg ttgctgtttt gccgattatt gcttattttg gtcgtaaaca
     5761 aaatgcattt ctgacgggtg tagggattgc atttgtatac ggtttttgtg gtgtgtttgt
     5821 tgctggaaga aatctgacag acttttatcc aatcacagca ggtcttggtc tagttggata
     5881 caacaatggt gctggtactg tttatcagcc acttattggt gctatgactt tgattgtaat
     5941 tctaatcttg acaactattc tacttgtatt tacacctaat tatgataaag ttatgaccat
     6001 atctaagaaa aaagggataa agcataaaaa gtattctcat aaataacata aatattgttt
     6061 ctattattta agttgctaga cagtgaaaag aaaaaatctg tcgtacagtg gctatatgct
     6121 catgtattca agaagctaca gttttgattt ttcataactg tagcttcttt ttgtatttat
     6181 taaatcacga ctatcataat acttgcaata ggtatagcga caagagagag tactgtcata
     6241 tgtgttttat atattacatt aagtatattc taaaatatat gttttaaata atatatttga
     6301 aaataggaat atttaaataa aaaaaataat attatatata gttgacaaaa attggcaaat
     6361 gatataatat ataaatatag aaatagtaat cattactatt attaaaaggg aggaattaat
     6421 tatgaactta aaaggaacta aaacagaaaa gaatttaatg gcaggatttg caggagagtc
     6481 agaagcaaga aataaatata catactacgc ttcaaaagca agaaaagaag gttataatca
     6541 aatagctgct atatttgaag aaactgctaa caatgaaaag gaacatgcta aattatggtt
     6601 taaactttta catgatggta tgccttcaac agaggaaaat ttaaaggatg cagcagcagg
     6661 tgaaaactat gaatggactg atatgtatgc aaaatttgct aaagaagcta gagaagaagg
     6721 atttgacaag atagcttatt tatttgaagc tgttggaaaa atagagaaag agcatgaaga
     6781 aagatactta aagcttttag aaaacttaaa tgaaggaaaa atatttaaaa gagatgaaga
     6841 agttgtttgg caatgtcaaa actgtggtca tgtgtatgta ggtactgaag caccagaaaa
     6901 atgcccagta tgtgaccatc caaaagcata ttttaatata aaagctgaaa attattaaaa
     6961 attatcatta ttaaaaagga atgttaacta tgaaattttc taaacaacga gaactgattt
     7021 taaatgaaat attaaataat ccggttcatc ctactgcgga ttacctatat gaaaacttaa
     7081 aaaaagataa tccaaattta agcttaggaa ctgtgtatag aaatttagct cagctaacgg
     7141 aacatggctt tataagaaag gttagtattc caggatatcc agatagattt gatggtcgaa
     7201 tagataatca ctatcatatc atatgtgaag tatgtggaga ggtatacgat ttagaatccg
     7261 aagttcttaa taatttacaa gaattaatat ctgaggagac agacataaaa ataacatctt
     7321 ataacataag ttttaaaggg atttgtaata attgtaaaag gtgtagccaa gtaggttagt
     7381 tttcaaattc taaaaataat taatgaggtg agtttatgtg tagtgaacaa aaatttttta
     7441 tatgtgagac ttgtggaaac cttgtaggaa tgatacaaag tggtggtgta cctattttct
     7501 gttgtggaaa acctatgaag gaacttgttc ctaatacaac agatgcagca gttgaaaaac
     7561 atgtacctgt aattgaagtg gatggaaata atgttactgt taaagtaagt agcacaactc
     7621 atccaatgac taaagaacat catatagcat gggtgtatct tatgactgaa caaggtggtc
     7681 aacgcaaatg tctagcagta gatggagaac ctgtagttaa atttgctttg aatgatgatg
     7741 ataaagtgat ttctgcctat gcttactgta atttacatgg attatggaag gctgaactgt
     7801 aatatttaaa taaattgagt caaaaaggag gctataaatg tattttcata caaatagcct
     7861 cctataaatt tatataaatt caagttttaa ttttgtttaa tgtcaatata atataaggag
     7921 aaaataatat gtcaatttat aaatgtagtg tttgtggata tatttatgat gaatctaaga
     7981 atgataaaac atgggatgaa ttaagtgaag attgggaatg ccctgtatgt acaaaaggtc
     8041 gtagttattt tggaaagata agtactgttt actatgaaga agatgaaaag atagcagaag
     8101 acatagttga agatgaatct aaattaaata ctgaaaaaga gggagattta aattacttaa
     8161 gtacatattt aagaagagat gatgaagttg agaaacatat ggatataatt cacgaaatgg
     8221 ctgttacagg aaaatctata atagaaccta tgagaactaa actaccagta atttcttggg
     8281 atgatatttt aattatgggt gctcaattaa atccattacc tttaaatgag catgatgaag
     8341 taaatacaac aacaataata ggaaaaaaag caaagaaacc tatgattata gaaaatccag
     8401 tttatatttc ccacatgtca tttggagctt tgtctaaaga actaaaaata gctttagcaa
     8461 agggtgcagc ccaaaataag acagctatgt gtagtggtga aggaggtata ttaccagaag
     8521 agaaggaagc ttcatacaag tatatttttg agtatgtacc aaataaatat agtgtaactg
     8581 aagaaaatct taaaaattct gatgctatag agataaaaat tggtcaagga acaaaacctg
     8641 gtatgggagg tcatcttcct ggcgaaaaag taactgaaga aatagcaaaa gtaagaaata
     8701 agcctgtagg tcaagatgta ataagtccat cttgttttga ggaaatacaa tccaaagagg
     8761 atttaaagaa acttgtagat gaacttagag aagtttcaga agggcgtcca ataggtgtta
     8821 aaatttcagc aggtcatatt gaaaaagata tggaatttat agcttatgct aaacctgatt
     8881 ttgtaactat tgatggtcgt ggtggagcta ctggagcaag tccaaagttg cttaaagatg
     8941 caacttcaat tccaacaata tttgctcttt atcgtgctag aaaatacata gatacacatg
     9001 gattagatat agatttggtt ataacaggtg ggcttcgtat atctactgat tttgctaagg
     9061 caattgctat gggggctgat gcagttgcta ttgcaagttc tgctcttatg gcagcggcgt
     9121 gtcaacaata tcgtatatgt ggttctggta agtgtcctgt aggagtagct actcaggatg
     9181 aggaattgag aaaaagatta catatagaaa acagtgctaa tagagtggca aactttttaa
     9241 atgtaagttt agaagaactt aagacttttg ctagaatttc tggtcataaa gatatacatg
     9301 atttaagtgt tgatgattta tatactgtta actctgaaat atcaaattat acaaacatac
     9361 agcatgtata gattgaagtt tgaaactatt agattttaga tagctataaa agtaaatgat
     9421 aaatatattt tataaaagag agtgtcttaa aatgaacttt ttagttcata agtcactctt
     9481 ttttttatga agccaaatat atttatcatt ttagcaatga aaaagaatct tatctcttta
     9541 ttttgagata gattccttct atttatgaaa attaaagata ttaaattgta aaatattctg
     9601 tgatgtcgta ctattaaact aaagattctg tactttaaag taaataaaaa tttaaggagt
     9661 gattattatg gaaagattga agatacaagt tttagtcgac aataatactt atattgatag
     9721 atactttgtt ggagaaccag cagtaagcta ttatattgag attgatggaa ataggatttt
     9781 gtttgactca gggtattcag atgtattcat atcaaacgct gaaaaattaa atatagattt
     9841 aggaaattta actcatgttg ttttttctca tgggcataat gaccatacaa gaggtatcca
     9901 gtttttagaa aataggtatg atttatctac aattgaactt atttcacatc ctaactgttt
     9961 tatacctaga aaacatggag agaaaagcat aggagctcca ttttctgctg aggaaattaa
    10021 aaatattttt atgtataatc ctaaggataa gccatttaat ctaagcaaga attgtgtgtt
    10081 tttaggtgaa ataccttcta taaatgattt tgaaaaaaga gctaaaattg gtaaatgtaa
    10141 aataggagat ttgtgggaag atgattatgt tttagatgat tctgctatag tttgtaaaac
    10201 tgataaaggt atttttatta ttacaggatg ttctcatagt ggaatttgta atatagtaga
    10261 gtatgctaaa aaggtatgta gagatgatag agttattggt atactaggtg gttttcatct
    10321 ttttgaattg aataataggt tagatagtac aatacaatat ttggataaag aagaaataag
    10381 aatgttgtac ccttgtcact gtgtttcttt taaggtaaaa gcgaagatga atgaaatatt
    10441 gaatataaat gaagttggag tagggctgac cataagtata tagtatttga aagtatataa
    10501 gatttaagtt gaaggttaaa aagatatttt ctatagaaaa tatctttttt gttacgttta
    10561 ttttaagaga aatcaatata cttttatatg aaatattgta aaaaaattta tttttaatga
    10621 gaatatattt tcattaataa acaattttat tatgtattaa taatgttaca ataaatataa
    10681 ataaatcaaa gttacttaca ttacagtata ttttttcaca aaa
//
LOCUS       DAGSHT010000114        10632 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.117, whole genome shotgun sequence.
ACCESSION   DAGSHT010000114 DAGSHT010000000
VERSION     DAGSHT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.117"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1035)
                     /locus_tag="MJB72_002499"
     CDS             complement(<1..1035)
                     /locus_tag="MJB72_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBY8263585.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYL"
     gene            complement(1311..3965)
                     /gene="adhE"
                     /locus_tag="MJB72_002500"
                     /gene_synonym="adhC"
     CDS             complement(1311..3965)
                     /gene="adhE"
                     /locus_tag="MJB72_002500"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBY8263586.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            4469..4669
                     /locus_tag="MJB72_002501"
     CDS             4469..4669
                     /locus_tag="MJB72_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263587.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(4876..5466)
                     /locus_tag="MJB72_002502"
     CDS             complement(4876..5466)
                     /locus_tag="MJB72_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBY8263588.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(5469..6344)
                     /gene="dpsA"
                     /locus_tag="MJB72_002503"
     CDS             complement(5469..6344)
                     /gene="dpsA"
                     /locus_tag="MJB72_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBY8263589.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(6420..7811)
                     /locus_tag="MJB72_002504"
     CDS             complement(6420..7811)
                     /locus_tag="MJB72_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBY8263590.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(7815..8951)
                     /locus_tag="MJB72_002505"
     CDS             complement(7815..8951)
                     /locus_tag="MJB72_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBY8263591.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(8983..9528)
                     /locus_tag="MJB72_002506"
     CDS             complement(8983..9528)
                     /locus_tag="MJB72_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBY8263592.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            9852..10631
                     /locus_tag="MJB72_002507"
     CDS             9852..10631
                     /locus_tag="MJB72_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263593.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         3598 a         1722 c         1234 g         4078 t
ORIGIN      
        1 aagatactta ctacattctc tataaaattt attttgattt cctatatatg taactttatc
       61 ttcataagcc aaacttaata tttctttttc attagatttt tttatataaa ttatataaaa
      121 aataattgca ataaatatta atacaataga taacactgca tatacagctc tgttgattat
      181 ttgttttgaa ttactaaata caacagtttc tggaattgaa aaaattaaat tccagtcttt
      241 tatataatta ttttcactat tcccattatc catagtggca tatcctaaat atcttctttc
      301 ttccaacata tttaatacag ttgcacctgt tttattttct tgcaattctt tttttaatct
      361 atttacttct tgaatatcat tatcctgttt taacaattta taaatatttt ttgataggct
      421 atccccttgt ggatgaagga taattgtacc attatttttt acaacataag atatactatt
      481 attttcataa aaagatatat taataagttt catcagagta ttgatattgt aagtacaata
      541 taaaactcca actactttat cattatttaa taatggtact gaaatagcaa tcttctttat
      601 agacttattt acttcatcaa catatggact tgaaacatac ttcttacctt taattgagtt
      661 tttgaaaaat tctctatctt tcactgaaaa cttatcacct ttggtatttt cagcattacc
      721 atttatatca actattgcca tagtgtaaaa tttttgattt tccatttcat ctaaaagatt
      781 tttcattttt tcctcattag gtatatctgg atttgaagca taatagttag aaaaaatatt
      841 taaaacatct aaatcttttt caatttgtct ttgtgctagt tgagagcctt gtactgcaac
      901 ttcttttata tgtatttttg cctctgacat aagaagatgt tttgtatctt ctatatagat
      961 ataccccatt gtcaaaaata atgatgttaa aaaaagaatt gtaattaatg taaaactttt
     1021 atttctccct gtcattcgac tcctccccta attagtcaat acttcatata gataatatat
     1081 cctttttata ccaattcatc atttatattg tatataacta ttcttaaatg ttacacataa
     1141 aatcaacata ataaccataa aaacattctt ttattattat aataacacta tttaattgaa
     1201 ttttaaatta aagaataaaa actatccatt ttctcaaaca aaaaaactct tatacaataa
     1261 aactttgttt tatgtataag agtttttatt ttatctattt tatagcaaac ttatcttcca
     1321 taatatgaat ttaaatacat atcttttatt tcattcataa gtggatatct tggatttgct
     1381 cctgtacatt ggtcatcaaa tgcttgaatt accatttcat ctattgaatc cataaactta
     1441 gattcttcaa cgccaaactc ttttatagat ttaggaatac caactttaag ttttaaatct
     1501 tcaatagcta ccaataaact ttggaatttc tcttcatcag aatttccttt taatcctaaa
     1561 aatgaagcta tttcagcata tctctttaat gcttctggat atttatattg agagaatgct
     1621 cccattttct ttggtgcatc tgaagaatta aatttcataa cttctgttat caaaagagca
     1681 tttgcaacac catgtggtac atggtggaaa gcacctaatt tatgagccat agagtgacaa
     1741 acacccaaaa atgcatttgc aaaagccata cctgccattg tagaagcatt tgccattttt
     1801 tctctagctt ctgggtcttt tgcaccatta tcatatgcac gtggaagata ttcaaatata
     1861 ctcttaatag cttgtaaagc taatccattt gtataatcgg ttgcaagcat agatacatat
     1921 gcttctaatg catgagttaa agcatctata ccagaagcag atgttaagct ttttggcata
     1981 ttcatcatca tatcagaatc aactatagcc atatttggca tcagttcata atctgctaat
     2041 ggatatttta ctcctgtatc ttggtcagtt ataacagcaa atggtgttac ttctgaacca
     2101 gtacctgctg atgttggtat agctacaaaa ttagcttttt cacccatttt agggaatgta
     2161 tatacccttt ttcttatatc cataaatctc atagctaggt cttggaaatc tacatctgga
     2221 tgctcataca atgtccacat tatctttcct gcatccattg cacttcctcc acctatagat
     2281 attattacat ctggattaaa ttctctcata gctttagcgc ctatcttagc acattccaaa
     2341 gttgggtctg gttctacttc aaagaatgta gtgtacttaa tattcatttc atctaataaa
     2401 tcagtaactg ctttagtata tccattattg taaaggaatg tatctgttac aataaaagct
     2461 ctcttcttat tcattacatc tttaagttct tttaatgcta ctcctaaaca accttttttg
     2521 aaataaactt tttctggtgc tctaaaccaa agcatatttt ctctcctctc agctactgtt
     2581 ttaatattaa gtaaatgctt aactcctaca ttttcagata ctgaatttcc accccaagac
     2641 ccacatccta aagtaagaga aggagctagt ttaaaattat ataagtcccc tataccacct
     2701 tgagaagaag gagtattaat taaaattcta caagttttca tggctgaagt aaacttatca
     2761 agtttttctc tttgatttac atcatcaata tataatgaag atgtatgccc aaatccacca
     2821 tcttctacta atttttctgc ttttctaact gcatcatcaa aatcacttgc cttgtacatt
     2881 gcaagtactg gtgaaagttt ctcatgagca aaagcttctt ctatttcaac tgattctact
     2941 tcacctatta aaaccttagt atctactggg acttcaaagc ctgcaagttt tgctatagta
     3001 caagctgttt gcccaactat tttagaattt aatcctccat ttactaaaat tatatttctt
     3061 attttttctg tttcatcttt atctaataaa taagcaccac gctctttaaa ttcttttttg
     3121 acttcattgt atatattttc taatactata accgattgtt cagaagcaca tatcatacca
     3181 ttgtcaaaag tcttagatac aagaattgag tttacagcag tttttatatc tgcactatca
     3241 tcaattattg ctggagtatt acctgcacca actccaatag ctggttttcc agatgaatat
     3301 gctgatttaa ccattccagg acctcctgta gcaagggtca aatccacttc tttcataaca
     3361 gttgtagtta attctaaaga tggctcatct atccatccta ttataccttc aggagctcca
     3421 gctaaaacag cagcctcaag aacaactttt gcagcctcta tagtagaatt ttttgctctt
     3481 ggatgtggag agaaaattat tccatttcta gtttttaaag ctaaaagtgc tttaaagata
     3541 gcagtagaag ttggattagt tgtaggtaca actgctgcaa taacacctat tggttcagca
     3601 attttagtca taccaaatgc ttcgtctttc tctataacac cacaagtttt agtttcttta
     3661 tatgcattat atatatactc tgaggcataa tgattcttta taaccttatc ttcagcaatt
     3721 cccataccag tttctttttg tgccattata gctagaggaa ctctttgctt attagctgcc
     3781 aaagatgctg ctaaaaaaat tttatctacc tgctcttgac tatatgtagc aaatattttt
     3841 tgagcttctc tgatttttgc tagtttttta actaagcttt caacgttatt tacagtatct
     3901 aaaccaactg acataacttc attttctttt ttattttcta ttattacttg ttctttatga
     3961 gccattttaa caacctcctg agatagtttt ttatttatta tgttaattta ttaacattat
     4021 agcatttact tttcctattt tcaagttatt tttttaacat tttatttttt atctaatata
     4081 acatcatttc tactaataat atctagcttt acaaatactt aatttaacta gatatcactt
     4141 tattttttac gcaaatcact aatgaaatca tctaaaaact taaggataac gttatttttt
     4201 taacgtttat atttttgtat tttttgcaaa taaaacttta taaaaagaat ttttaatatg
     4261 atactctatc aagtttaaaa ataaaaatcc ttgaaaatga aaattaaaac ttaattaaca
     4321 agttaaagta aatatagtta aatgtacaaa catttacaac atatagtata attaaaaaaa
     4381 tatatattca tgatttttat ataatatgaa ataaaatata tttaatatta tatagataaa
     4441 tttgaagttt ataagaagga ggataagcat ggaaaaatta aaaacaatta gcgttttttc
     4501 tttgattata agtgtaattt taacgattgg aggtattgga atagtaactt attatgtaga
     4561 taatttgttt attagaggac tatcagtatt tgttttaatt atgtcctctt cttttgtatc
     4621 aacaacagtt agattgatat ttgaagagtc aaaacgatat aaattctaat ttaaaaattg
     4681 gattaagtaa ataattttaa tatagcaaaa attacggaga ttagctcaaa atagactttt
     4741 tagtccatga ggcatttttt tgtatgaaat taaatatatt ttcatcattt caacaagaaa
     4801 aagaggcttc tctctatttt gagacagcct ctttgctagt attgtatcta atacataata
     4861 ttataagaac caattctatt gatgtggact ttttataaca ggctgtagct gtttgttctc
     4921 tagagcaagt tctatagttg gaattattag ctcagtatgt gcaatcatag aattaggctt
     4981 tgctttgcaa ttatcttgac caaaaggtac aaaatatata tttttagaat taaggagaat
     5041 gccaatattt ttgaaattaa agcttagagc atcattcgtt gatattgata ttactagtgg
     5101 tttatcatta cgaagatgac cttttgctgc cattaatact ggtgaatctg taataccatt
     5161 tgctaatttt gcagcagtat ttccagtaca aggagctatt attataatat cagcaattcc
     5221 tttaggtcca aatggctctg cctcttcaat cgtaactata ggtttatttc ctgtaagttc
     5281 atatgctttt ttcataaact cttcagaatt tccaaatcta caatcgatat tttgtgatac
     5341 atctgaaaat atagtgtgta cattagctcc ttctttaacc aaattctcta actctataaa
     5401 tattttatca taagtacaaa acgaaccagt aaatcctact ccaattgtaa gtccttcaag
     5461 tctcatttct aatcccttct ttcatttata atattttcta tagcgttaac aagaatttca
     5521 ccagatgtct ttggtgaata tttaccagga agcccaaggc aaagagtcgc attaatccct
     5581 gcttctttaa caacagaata atctactcct ccaggagaag aagctatatc tataatagtt
     5641 acttcttgtg atactttttc taacctttct ttcgttaaaa tacactctgg tatggtgtta
     5701 aaaatataat caaattgtaa aagtttttca tcaagttgac ttaaagaaat actggaatat
     5761 ccaaatgcat tagccgtact tcttgcatca ttatttcttg cgccaacaca tacatttgca
     5821 tccaaagcgt ataatttttt tgcaagtatt tgtgcacatc ttccataacc taatactaaa
     5881 cagttacttt gatgtatatt gattatactt cttttaatag cttctgctat agccccttca
     5941 gctgtagcaa ctgcatttaa aatagcaaca tcattcattt tcataaaatc ataaatctga
     6001 atagagtgtt gctcgcaaaa tttacaaact ttagttggta tgttacctcc aaataatatt
     6061 tgattaggag ataaattaat tagcaagtta tcaatagtag catcaagaca atcatccgta
     6121 ttttcaatat taacttgatt ctttgtaaat ggaattggac ataaaataat ggaacttttg
     6181 gatagtgctt cagctagtga agttgatttt ccaacgattc catttataat ttcatttgat
     6241 atcccataag tgactacaga atacccttta ttaattagtt tttttaccat gtacacttgt
     6301 cgcaggtctc ccccaataac agttatatcg tattgatttg acatattgta agcctccttt
     6361 actatataca atatgttttt atcaatacat tggttcacta aaattactaa atattatact
     6421 tataacattt ttaataatgc taatctatct atcttttcag aagaggtata tggaaatgaa
     6481 tttaaaaaga taattttctt tgggatttca tttttcatta gattatcttt cattttaatc
     6541 aacaaatctt tcttcgtaat tttgtctata gttattacaa ataatacaat ttgatttcct
     6601 cttatagtgt ctgagtacgg caatacaatt gcatcttcaa tttgaggaat ttttttaact
     6661 tcattttcaa tcttagtaga actaacttta aatcctccaa tatttataat gtcatcactt
     6721 cttccttcaa atattaagta tccatcatta tcataatatc caatatcatt gacagaataa
     6781 ggtttggttg cattatatac tgcatattct gtattcacaa aaatttttcc ttctctaata
     6841 tatatatcta ttcctggaaa tggtctgcct acacttagag gtttttcaat tagttcatca
     6901 tagcataaat atgtaatata atttaattca cttgccccat agtataaaat aatttcagac
     6961 tttggcatat atttttttag atttcttgca gtatcttcaa ataataattg agaccctgta
     7021 aaaatactta caactttgag tactggttct tttaagtgtt taaccaaaag ttgaagtttt
     7081 gtaggtataa ggtatatgtt agttacatta tattgtttta ttgtccttat ccatgattta
     7141 caattaagac ctgaacttat tacaatacta cctccttcat ataatactga cattatagaa
     7201 tttagattcc ctgtaaaact aagtgttcca ttaacaaaca agattgattc cccagagata
     7261 ttaaaaatac cattttgtat aggaaaaaat ccagcccaac tctcatatgt tctatataat
     7321 acctttggaa cacttgtaga acctgaagat aaagctccca tacaaatact tttatctaaa
     7381 taatttataa gcttcttata ttgatacata tatatattaa atttaggatt aaaaatagaa
     7441 aaattaactt tttttgctac ttcattattg ttatcatcat ataaatcaat aacgtccata
     7501 gattcatcag atataatgat tgaaatatta ttttttaata aaatatcatc taatactttt
     7561 ttagaaagat tataatgaca aataatagga atattttttg aataattaat agcaaaaaaa
     7621 ctaatcaact gaaacattat atttttagaa tatattaaaa ttgggatatt ttcacctaaa
     7681 gaaatttgct ttcctagttc ctcactataa ctcaatatat tttcataagt atatttttct
     7741 ccatctataa ttaaaaaatt tttattttta tgcctctctt tttttattct taaaagttca
     7801 taatagttca taatctacac cctttcaagc agtaaagctg aacctagacc accagctgca
     7861 gctatagagc aaattccata tctaccctta gtcttttcta atgcttttaa aagatgtaat
     7921 gcaataattg cacctgaagc accatatgga tgtccatacg ctaaagctcc accaaatata
     7981 ttatatctat ctattaactc tggatacttt ctttgaaaca acacatctat aactgcaaat
     8041 gcttcattaa attcaacagc agatacatct aaataattaa ggctttctat ctctaaaagt
     8101 ttatccattg cttgtatcgc tgatgttgga cttaaggttg catctgttcc tatcgtgcat
     8161 gtgttaataa tttttgcttt aggcttcttt tttacatgtt ctaaatactt cttagaacat
     8221 attataataa atgaagctcc atcattaata agacatgaat ttgctgcatt tgtaatagtt
     8281 tcttttccta aaattgatgg catcctatct aataaccttt gactcatctt atcacgtata
     8341 ccttcatcat aggtactatt atttatagga gatataatat cttcaagtat tttttcctct
     8401 cttgcttctt ttgctctttt atgactttcc tttacccaaa aatccaagtc tgctttctca
     8461 atttgatata actctgctac tctttccgca ccttcgagca tactattttg gctattatca
     8521 tcaggagaaa attgagcaac tgtataattt ggattatttg tattatatcg cttatcattc
     8581 ttatgatatg tgcgcattgg ttgcagagaa ctactttcaa aaccacctgc aataattaaa
     8641 tcacactgac cagatttaac ctttgaaaaa gctatgtcta tactcatcat agcagaagca
     8701 cactgcatat ccacagtaaa agcaggtact tcatttgaca ctcctgctgt taaagtcatt
     8761 aatctagcta tattccctcc tgtcccaact gcatttccac atataatctc atcaattttg
     8821 tcaatttcat actttttaat taaatctttt aaaacacttg ctcctagtaa ttcaggttga
     8881 acaaattgaa aaattccatt ttttagacct atatgacttc tcaatcctcc taatataaaa
     8941 acttccttca tacattacac ccttccttat attatagatt tatcatagat ttcttctgag
     9001 tacattgttc agtttagtgc ctaaaaaaga tgcaataact gactttattg tatctccaat
     9061 taaaaatggt actactgctg caaaaagtgc tgcttctact gtcatttgat taaaatagca
     9121 cataaatact gttcccataa aatatataat aggcattcca acaaaaattg ttattaatat
     9181 atatctctta atatcattat ttcgtccttt taataaacta ataattagtg ctgcaaacaa
     9241 aaacccaaaa taaaaaccac ctgttgggct taataacttt ccaataccag atgtacctcc
     9301 accaaatact ggaatcccaa ttaaacctat cattatatat acaccaactg tactaaatgc
     9361 ttgtcttggt gttaaaatca aagctattaa atttatcatt attgtttgtg ctgttactcc
     9421 tgctggagta aatggcaatg gtatatatat gtaagatgat atacacaata atgctacaca
     9481 tattgacatt ttagttaaat cttgaatatt tactttagta tttttcattt ttataattcc
     9541 cttcatatgt cttatttcat ttagtttata tgtacatata caaagcttct attaaagctt
     9601 tgttttagta taaaataaag acgttttttt gtcaactatt ataattattt aggtttacat
     9661 ttaatttata agtaaacttt atactgttac tttatatttt aatagtcatt tataaaactt
     9721 tgcttaaaaa cagctctatt tagactatac ttgtaaatta taatatgata tatttttata
     9781 taagatttta ttttatataa aaaatagtaa caaatagttt atacacaaga tatttaaaag
     9841 gaagtgatga tatgaaagtt aatgaaaata atataaataa tatagatgaa gagttagatg
     9901 aacttagtag gaaagtttac ttagagtcac cagatttttg gataagtatt tctgacaaaa
     9961 taatagataa agtatttgat gaaatgttat tattttttgc agttaaatat aactatttca
    10021 atatagtatg ctttgctatt gaaaacaatt atctggattt aaacatgcct tcaaaaaaca
    10081 aaaaatacac taatataatg gagcatttgt tagatacatc aaaacagaat gaagataaaa
    10141 aaatttataa ttatcttcta agcttaaata aaggtaataa cctagatgaa agaccaattt
    10201 tagatgaatt aaactgtaat attagtagtg atacttttga aaaaaaagat atatatcttc
    10261 caaagtactt atgtccaagt tgtaattcta atatatttga tacaggttat aaagtatcaa
    10321 atgacataac atataaattt tcttctaaag aagctgaacc tattgaaatg tgtagtgaag
    10381 tttcttctat cagatgttgt aattgtgatg aattattaga agatattaca cttgacaaac
    10441 tatataacct atgcaaaatt caaaactgta ataattgtgg ttctaattta actaaggtag
    10501 gaattattgc aaagaaaaaa atgaactttg atgagaatac tgaaaaattt aaagatgtct
    10561 caacatctta ctgttgtgga aattgtgact ctgaaataaa cttatctcaa agagaacatt
    10621 ttaagattta aa
//
LOCUS       DAGSHT010000115        10570 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.118, whole genome shotgun sequence.
ACCESSION   DAGSHT010000115 DAGSHT010000000
VERSION     DAGSHT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.118"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..2179
                     /locus_tag="MJB72_002508"
     CDS             <1..2179
                     /locus_tag="MJB72_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBY8263594.1"
                     /translation="FLSGKISIDNGQMSLFSMYDFNIIPVELISNIYEILLGKEIRNK
                     DNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSCGSGVFLVNSFRRIIDMNLGDKL
                     FCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLTILDYKDPKILSEFKLPNLKGCN
                     LIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGLHLDYCNKNGYNDMQQNKEISRS
                     FIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFREFLLKKSQIFSVVEMSSVRKLVF
                     KNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFFKLFNIIVIEKYDVKYISQKLLL
                     ENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILKNSFVHGTGIQINGNESHDATHL
                     KGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKANQQEIFIPPYVLLKKGVDTKTYR
                     YRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGMLNSSFYSYLNIMLGTSIGIEREQ
                     GFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMFLNEKNSCKYIKQLDKLILKSFN
                     LNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLINYSECFKEQFSSIYRRVGKSIS
                     ISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNNMELFTKIIRYDYNDVFHQIRDI
                     IHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQILSDTGGEE"
     gene            2179..2853
                     /locus_tag="MJB72_002509"
     CDS             2179..2853
                     /locus_tag="MJB72_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263595.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            3014..3430
                     /locus_tag="MJB72_002510"
                     /pseudo
     CDS             3014..3430
                     /locus_tag="MJB72_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            3390..5837
                     /locus_tag="MJB72_002511"
                     /pseudo
     CDS             3390..5837
                     /locus_tag="MJB72_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            5977..6399
                     /locus_tag="MJB72_002512"
     CDS             5977..6399
                     /locus_tag="MJB72_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263596.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            6937..7971
                     /locus_tag="MJB72_002513"
     CDS             6937..7971
                     /locus_tag="MJB72_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBY8263597.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            8058..8261
                     /locus_tag="MJB72_002514"
     CDS             8058..8261
                     /locus_tag="MJB72_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBY8263598.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            8432..9592
                     /locus_tag="MJB72_002515"
     CDS             8432..9592
                     /locus_tag="MJB72_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBY8263599.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            9851..9991
                     /locus_tag="MJB72_002516"
     CDS             9851..9991
                     /locus_tag="MJB72_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263600.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         4370 a         1038 c         1765 g         3397 t
ORIGIN      
        1 ttttttatca ggtaaaataa gtattgacaa tggacaaatg tctttatttt caatgtatga
       61 ctttaatatc ataccagttg aactaattag taatatatac gaaattcttt taggaaaaga
      121 aattcgtaat aaagataacg cattctatac tccgaattac ttagcagaat atattttaga
      181 taaaacaaca cttgaatttc tgaaaaataa taggcaatac aaaattctag atccttcttg
      241 tggatcagga gtatttttag taaatagttt tagacgtatt attgatatga atttgggtga
      301 taaacttttt tgtgaagatg ataaactttt aaaagaactt ttattatcta atatttatgg
      361 aattgatatc aatgaagatg ctatcgatgt tactattttc tcgttatatc taactatttt
      421 agattacaaa gaccctaaga tattatcaga atttaaactg cctaatttaa aagggtgtaa
      481 tttaatagcg agtgattttt ttgatgataa taaattggag agtttaaaga aaattaactt
      541 tgattttatc attggaaatc cgccttgggg aagtgtctca gatggattac atttagatta
      601 ttgcaataaa aatggctata atgatatgca acaaaacaaa gaaatcagta gaagttttat
      661 ttttagagca aaagatttta gctctagaaa tacagtatgt tcttttgtag tgcattctaa
      721 aatactatat actcaaaaaa agccatcaaa aaaattccgt gaatttttgc ttaaaaaaag
      781 tcaaatattt agtgtagttg aaatgtcatc tgtaagaaaa ttagtgttta aaaatgcaga
      841 cgctcctgca gcagttatat catttaagta taacgaaagt gaagaaaatt taaataatag
      901 aatcatctat acatctttaa aaccaaatgt attttttaaa ttatttaaca ttattgtaat
      961 tgaaaaatat gatgttaaat atatttcaca aaaactatta ttggagaatg attgggcttg
     1021 gaaaactatt gtgtatggat tcagtaaaga tatagatata attatgaata tcaaaaaaaa
     1081 atatcaaact gtaaatgaaa ttattttgaa aaacagtttt gttcatggaa cagggataca
     1141 aatcaatggt aatgaaagcc atgatgcgac acatttaaaa ggaaagtggt atgttgattc
     1201 aaagaaagga atagattcat tctatgttga tataaataaa gggaaagaac ttactcaaga
     1261 taaaatgaga agacctaagg ctaatcagca agaaatattt attcctccat atgtattatt
     1321 aaaaaaggga gttgacacga aaacatatag atatagagca gcattttcaa atgatgcatt
     1381 tgtatattca gatgcagtta caggaatatg tggaaaaaaa gataatttaa ctacacttta
     1441 ttgcttgact ggtatgctta actcttcttt ttactcatac ttaaatataa tgctaggtac
     1501 atcaattgga atagaaagag aacaaggatt ttcaacggaa atttttacgt atccagcaat
     1561 agaaagtgat gaaattgcta atctggtagt aaaaatacaa gaagcgacta aattagaaaa
     1621 aacaactatg ttcttgaatg aaaaaaatag ttgtaagtat atcaaacaat tagataagtt
     1681 aattttgaag tcttttaatt taaatgatga taaatttatt gactatgcaa taaatattca
     1741 aattcctgag cttacaaatt ctaagtcaac taacatatat agaaaaataa ctaaggaaga
     1801 tttaattaat tatagtgaat gtttcaaaga acagttttca tcgatatatc gtagggttgg
     1861 gaaaagtatt tcaatttcgg tatatccaaa aatattaaat cgttttacag tatttgaact
     1921 agaagtaaca gatgaaaaaa cagatagaga gattatggtt aaagcagaag ctgataataa
     1981 tatggagttg tttacgaaaa taattagata tgattataat gatgtttttc atcaaattag
     2041 agatattatt cattttggag aaaattcatt ttatatcata aagcctaatt gctataaata
     2101 ttggcatcct gctatcgcag aaatggatct tgctgatgtt ttggatcaga ttttatctga
     2161 tacagggggt gaagaataat gggaggattt aaaaattctt cactaaactt ttatttaaag
     2221 caagggcttt atgaaataat tatagatgga ttaatagact gttgtttact tatgagcaaa
     2281 tcatgtattg aacaatctag aaaaatatta aatcacgaag aaaagataag aacgcattta
     2341 gttgaaaatt atttagaaaa tgatattgaa agaagaaaaa catctttatc taaacaacat
     2401 ctaagatttg aagtagaagc acaagaaaat tatgacaaca aaaatgaatc ttatgagggt
     2461 agggttgata taaaagttat atctaaagac actttagcgg atagaagggc ttattatatc
     2521 attgaatgta aaaggataga tggtaaggaa actttaaata aaaaatatat aaccgaaggt
     2581 gtacaacgat ttattactga gaaatattct tcgcattatg gcaaaaatat aatgcttgga
     2641 tttatagtaa aagatataaa tatccagagc aatatacaaa aaattaattc actgaatcag
     2701 aacatattat atacatatat agatacagat atgttgacag ttagaagtga ttctgaaaat
     2761 aattatgctg tttgtgaaag taaatataaa gtaaaatcta aactactatt gttaaatcat
     2821 attttttata acttttcgag tataataaga taatcttatg aatataaaat attactatgt
     2881 taaaattata gtttggtttt gataatatta taaaaataag tttaagaata aaatataaag
     2941 gaaaaaaggg gcgtagcact attttttagt gtgacagccc ctttaatttt aaaatcggta
     3001 aggaggagtg tatttgaaaa aaattaagtc atatacaggg attttgaatg tggagaaggt
     3061 tatatatgca ataaatgatt ttcagcttcc atttccagtt acttttacgc agatgacatg
     3121 gtttgtatta acacttttta taattatatt atttggtaag ttacctccat tatttatgat
     3181 agaaggagca tttttaaagt atcttggcat accaattttt ataacttagt ttatgtctca
     3241 aaagactttt gatggaaaga aaccatatgg atttttaaag tcaataatac tatattcatt
     3301 aagacctaaa ataacatacg caggaaaaaa cattaaattt aaaaataaaa ttattgatga
     3361 aaatataact atagtaagga gtgaattata tgttcccgat aaaatatata gagaacaatc
     3421 ttgtatttaa taaggaagga gaatgttttg catattatga attaatacca tataattatt
     3481 cattcttata agctgaacaa aaatttattg tacatgataa ttttaggcaa ttaatatcac
     3541 aaagtagaaa tggtaaaatt catgcacttc aaatagccac tgaaagttct ataagagtta
     3601 cacaagaaag gttaaagaaa aatattacag gaaaattaaa agaaattgct tatgaaaaaa
     3661 tagacgagca aacagaggca cttgtttcta tgattggcga taatcaaatt gattatcgtt
     3721 tttttattgg gtttaagctt ttagttacag agaaagaaat gaatataaag agtataaaaa
     3781 agtcaatatt agctaatcta tcagatttta tatatgatgt taataataaa ttgatggatg
     3841 actttgtcag tataaataat gatgaaatta tgagattttt aaaaatggaa aagttattag
     3901 aaaataaaat ctcaagaaga tttaaagtta gaaggttaga taaaaatgat tttggatatt
     3961 taattgaaca taattatgga aagaaaggca cagtttttga agattatcaa taccatttac
     4021 ctacaaagaa acttaaaaga gaaacattag taaagaaata caatcttata aagccaacta
     4081 gatgtcttat agaagagaag caaagatact taaaaattat acatgaggat tcagaaagtt
     4141 ttgtagctta ttttactatt gaaattatta ttggagaaat ggattttcca tctagtgaga
     4201 tattctatta tcagcaacaa cagtttgcat ttcctattga tactagtatg aatgtggaaa
     4261 ttgtaacaaa taaaaaggct ttaactacag ttagaaataa gaaaaaagaa ttaaaagatt
     4321 tagacaatca tgcttggcag tcagggaatg aaacagtaac aaatgtagta gaagcattag
     4381 aggctgtaaa tgagcttgta aataatttaa atcaaacaaa agaatcaatg tataagttaa
     4441 gctatgtaat tagggtatca gcagatagca ttgatgaatt aaaacgccgt tgtgatgaag
     4501 ttaaagattt ttatgacgat ttaaatataa aattagtacg cctaatggga gatatgatag
     4561 gatttcacaa tgaatttata ccttcaagca aaaggtatat aaatgattat attcaatatg
     4621 taactagtga ttttctagca ggattagggt ttggagcaac tcaatagctt ggagagaacg
     4681 aaggaatata tattggatat agtttagata caggaagaaa tgtctatctt caaccatctc
     4741 ttgcaagtca aggagtaaaa gaatctgtaa ctaatgcgtt ggtagcagca ttctttggat
     4801 ttttaggagg tgggaaatca tttgctaata acttacttgt ttactattca gtattgtttg
     4861 gagggcaagc tgttttggtg gatccaaaag ctgaacgtgg caaatggaaa gaaaatttac
     4921 cagatatata tcatgaagta aatattgtta atttaacaag tgatatagaa aataagggaa
     4981 tgctagatcc ttatgtaatt atgaaagaag taaaagatag tggaagtcta gctattgata
     5041 tattaacttt tcttacagga ataagtagtc atgatggaga gcagtttcct atacttagaa
     5101 aagcaatcat aaatgttact caagacaaat gcagaggact tttgaaggta atagatgaat
     5161 taaggaaaga taattctcaa atatcaaata atattgcaga tcatattgaa agttttacag
     5221 attatgattt tgcacaatta cttttctcag atggaaatat taaaggctca attaatctag
     5281 aaaaacaaat gaatataata caagttgcag atcttgtttt accgaataaa gatactttat
     5341 ttgaagaata tacaacaatg gaaagtcttt cagtagctat gttgatagta attagtactt
     5401 ttgcattaga ttttatccat acagatagaa gtacttttaa aatagtagat ttagatgaag
     5461 cttggacatt tcttaatgta gcacaaggaa aaactttatc taataaatta gttagagcag
     5521 gatgtgctat gaatgcagga gtgtattttg taactcaaaa tgcaggtgat gtatcagatg
     5581 aaaatttaaa aaacaatatt ggattaaaat ttgcattcag aagcacagat atcaatgaaa
     5641 ttaagagaat acttgaattt tttggagttg ataaggaaga tgagaataat caaaaaaggt
     5701 taagagagtt agagaatgga cagtgtttaa tacaagattt atatggacga gttggggtaa
     5761 tacaaataca tctagttttt caagaactcc ttactgcatt tgatacaaga ccacctataa
     5821 gaaaagaggt ggattaatga aatcgaaagt atttagaaat ttaagctgga taactatttt
     5881 aatagtagta cttttactat tttttattta tataaattta tagatttaga gaaacaaata
     5941 gatacattag ataagaaaag aaaaaaactc atagatatga gattagaaga tataattgat
     6001 aaagaatcat atgaagaaaa gtatgatgat ttaacagttg aaataaagaa acttagtaaa
     6061 gagaaaaaaa ctcttaaagc atcattaata aatgaaaaga gtataaaagc aagacttaga
     6121 gaatttagaa agatacttga aagaaatgaa ttgttagaaa aatttgatag atatgtattt
     6181 gcaagtatag tagataaggt tatagttgga gttattaatg aagatggtat agttgatcct
     6241 tataaaataa cttttgttta taaaactagc tttgatgata aacaagatgg acataaattt
     6301 ataaataaga gtccgttaga aacgtattca aaagataaca acgataataa aaagaagtgt
     6361 tcatatacta ataatgagac attgcgagac tgtagttaaa cttattagag aataacaagg
     6421 tttcagactt ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta
     6481 tttttgggtg gcaaatttat tctacaatga tttcaaaaat atttaccgtc tggtttctca
     6541 tgttgttagt tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta
     6601 tatgctggat atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa
     6661 aataaagtgt gtattcttct aaagtagtga taattaaaat atcaaatatc actatacaaa
     6721 ggaaagtgca cgctttttat gtttaacaaa aacgaaatat taaaaaactt attgaagaat
     6781 atgatgtcaa aacaactaca gatattcaag atatgctaaa agatttattt acaaatacaa
     6841 ttcatcaaat atttacattg tcgaatttta gcggtttaaa cataatatat tgactgatat
     6901 aataaaatca tagaatattt tagggggaaa tagattatgt ttaagaaaaa gttaatagct
     6961 gttgcaatga gtataactat gattagtgta gggagctttt cgtatgctca ttcaggaaga
     7021 actgattcat caggaggtca tcgtgataat aaaaataaat ctggattagg aagttatcac
     7081 tatcattgtg gtggttatcc agctcattta cataataatg gtggttgtcc ttatactgga
     7141 ggaggaagca gttctggaac aacaacttcg gttaataatg aagaaaagca aaagaggtct
     7201 gttggagaaa aaggatataa tcaagggtat gaagatgggt ataaaggaaa ttatagttca
     7261 tcaaattata gtggagatta tagtgataca tatgaatcaa agtactcaga aggatatgaa
     7321 aaaggtaagg ctaaattaga agaggaagaa aaggtggcta aagagactgg atataattta
     7381 gggcttacag gtgctaaatc taataataca tatgaaaaag aagttttaaa aaatgcttat
     7441 gataccggat attcaactgg atacaatgaa tataagacta aaaagataga agaatataaa
     7501 gctaaaggga tagaggatag caataaagat aaggaaaaaa tgacatttga agaaaacata
     7561 gattctgaat ttatagatgc ttataataat gcttatgacg aaatacaaga acaattaaaa
     7621 aatgattaca ctactcaagg atttgagagt gctatcaaag gagaaagatt tgacacatca
     7681 actataggta atgttaaata tgctaattgg tttaaagaag gatatgataa tggtaaggtt
     7741 aagttaccta aagcaaaaga aagtgtatat aatcaaggtt acaatgaaga agattttagt
     7801 ataccagatg aaatgaagtc tattgaaact aagttaaaag gtgtatatga tgaaggttta
     7861 gaaaaaagag aagaagagaa aagtagaaat gctacttatg gggttggagc tggaacagca
     7921 gctgtagttg ctggagtagt atataagaag aaaaaacgta aaaagatata gatgctagat
     7981 ataccaatga tgacaagtgt ataacaaata taattttaag tataaatatt taaaatatag
     8041 aagggaaggt tatatctatg ggatttaact ttaggaaaag cataaatcta ggaggaggac
     8101 tcaaattaaa tttaggtaag aagagtgtag gtataagtgc tggtgttaaa ggtgctagag
     8161 tgtcagttaa ttcaaaagga agaaaatcag ctacattgag tatacctgga actggaatat
     8221 catatacaaa aactagtaca aagaaacgta aacctaaata aatatttgat atactaagaa
     8281 atagactgtt acaaatagtc tattttttag tatatagatt taaaaagtga caaaattttg
     8341 aaatataaac taatttcata aaggaaaata ttattttatg tagaattata atatttaagt
     8401 aatattataa aatttatagg aggatttcat tatgaaatta agcaaaatac tattggcaat
     8461 agggatgtct gtttcattat tgattgctag ttgtccaaca tcaaacgctc ttagttctat
     8521 taattcaata aaaggtacaa ataagtatga aacagcaggt ttaatagcag ataaacaaaa
     8581 ttatgatatt gcaatactta ttaattctga aaatggatta tctgatggat tgtcagcaag
     8641 tggattgtca ggagctaaaa atgccccaat attgttaact aagaaagata caatcccttc
     8701 agaaacatta aaaagattag aaaaggtaaa agaagtatat ctaattggag gaaaaaattc
     8761 gataggagca tcagttcaga aaatattaag tgataaaaaa ataagcataa atagaataag
     8821 tggaactgat agaatagata caagtcataa agttgcaaaa gaaataggaa gagtaagtac
     8881 ggttaaaaaa gtaatattaa ctaatgcttt taaaggcgaa ccagatgcta tgagtgttgc
     8941 ttctatagca gttagagata aatctccaat agtattaact gatggaaata ctacaagttt
     9001 tgctacagat ggattagaaa cttatgcaat tggtggaaaa gtcagtatga gtgataagtt
     9061 aataaatgaa actaaagcaa caagaatagg tggagtagat agatacgata caaataaaca
     9121 aataataaat aaattttata atggctcaaa agaattttac atagcaagtg ggactgattt
     9181 agtttattct ttagttggtt ctacaattgc acaaaaaaca cctatagtgt tagttggaaa
     9241 tggaagtaat aagagtatat taaaaaatgc aagtaaaata actgctattg gaaatattga
     9301 tgatgttgtt ataaaacaat gtttaaatat aactaataat gttggggatt caaatacagg
     9361 tattgtaaaa gatactaatt caaataatac gaacaaacca agtaatgagc ctagctctaa
     9421 taatcctgga ttaactgata aaagtacagt atatgtaact cctaatggaa aatcatatca
     9481 tctaacaaaa aattgttcta cactaaaaag aagtaaaaca ataaataaag taacattaaa
     9541 acaagctaag tcacaaggta aaatagatcc ttgtaatgtt tgtgtaaaat aatgtattag
     9601 ccaaatatat attaaaaaag acaattaatt aagattgtct tttttattga agaaatataa
     9661 atttatatta tgtattatta aaaaaaaatt attaattata aaaaataata ataaagacga
     9721 taagtattaa aattttatgt ttccaatatt tatgatataa ttaatatggc aaggaataaa
     9781 tatttgaaaa agctatgagt ggtgtttcca tggaagtttc ttcactttct tatgagagga
     9841 ggtgaaaact atggaaaatc taatgatgag tattataact ggtgttatag ccagttacat
     9901 ttacgataaa ttaaagagcc actctgagcg accaactaag agtggctggg aacttaatat
     9961 aaagttccag aaaagtaaat cttcaaaata atttgttgaa ggaaacatca ctcaaagtta
    10021 agtaaaatat gtttccttgc ttttattata tcacaaatta aaaatattaa cacaaaattt
    10081 tgtgaatata tacttattat tgttctaaaa aaatatgtaa ataattgatt tgattgcatg
    10141 gaattattga tgtaatattt aagttaaata tacacaatct caattaaaga agataagtac
    10201 tataaattca tatcaaaaaa atacataaac ttttaagtta taattttata aaaagtcttt
    10261 tagtatcatt atttcaagag gaattatcat atgaaaaaat aacttcatta gaaaagtgga
    10321 taaaaaactg gtaagaagat gactaaagat tacatatact attacttaaa ttaaaaattt
    10381 aatataattt ataatgtata caatttatta taaaaaattt aattttaatt ttctattttt
    10441 gatactataa tatataatct tgataattta attaaaaatc tagattttac ataccaaact
    10501 ggtttcttat ttcgctatct ttacttatga actcatatat tcctataaag gttattttag
    10561 aaaaagttgg
//
LOCUS       DAGSHT010000116        10544 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.119, whole genome shotgun sequence.
ACCESSION   DAGSHT010000116 DAGSHT010000000
VERSION     DAGSHT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.119"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..686
                     /gene="nadC"
                     /locus_tag="MJB72_002517"
     CDS             <1..686
                     /gene="nadC"
                     /locus_tag="MJB72_002517"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBY8263601.1"
                     /translation="VFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGE
                     RVALNYLQRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHR
                     FNLSDGILLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADII
                     MLDNMSLEMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALD
                     ISMKNLRNI"
     gene            942..1274
                     /locus_tag="MJB72_002518"
     CDS             942..1274
                     /locus_tag="MJB72_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBY8263602.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            1436..1621
                     /locus_tag="MJB72_002519"
     CDS             1436..1621
                     /locus_tag="MJB72_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263603.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            1648..2733
                     /locus_tag="MJB72_002520"
     CDS             1648..2733
                     /locus_tag="MJB72_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBY8263604.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            2726..3184
                     /locus_tag="MJB72_002521"
     CDS             2726..3184
                     /locus_tag="MJB72_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263605.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            3219..4310
                     /locus_tag="MJB72_002522"
     CDS             3219..4310
                     /locus_tag="MJB72_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8263606.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            4315..4539
                     /locus_tag="MJB72_002523"
     CDS             4315..4539
                     /locus_tag="MJB72_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263607.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            4549..5112
                     /locus_tag="MJB72_002524"
     CDS             4549..5112
                     /locus_tag="MJB72_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263608.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            5073..5702
                     /locus_tag="MJB72_002525"
     CDS             5073..5702
                     /locus_tag="MJB72_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263609.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            5742..5879
                     /locus_tag="MJB72_002526"
     CDS             5742..5879
                     /locus_tag="MJB72_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY8263610.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5879..6631
                     /locus_tag="MJB72_002527"
     CDS             5879..6631
                     /locus_tag="MJB72_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263611.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            6644..7129
                     /locus_tag="MJB72_002528"
     CDS             6644..7129
                     /locus_tag="MJB72_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263612.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            7136..7285
                     /locus_tag="MJB72_002529"
     CDS             7136..7285
                     /locus_tag="MJB72_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263613.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(7421..8242)
                     /gene="yidA"
                     /locus_tag="MJB72_002530"
     CDS             complement(7421..8242)
                     /gene="yidA"
                     /locus_tag="MJB72_002530"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBY8263614.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            8735..10429
                     /locus_tag="MJB72_002531"
     CDS             8735..10429
                     /locus_tag="MJB72_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBY8263615.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
BASE COUNT         4127 a         1082 c         1859 g         3476 t
ORIGIN      
        1 gtgtatttaa gagagttttt gagatacttg gtgatgtaga tgtaaaatta tataaaaatg
       61 atggtgatag agtaaagaat cgtgaaaaga tagctgtatt aacaggtagt actagaaact
      121 tacttgtagg agagcgtgta gcattgaatt atttacagag aatgagtggt atagctacta
      181 ttacaagtag gtatgttgaa aagcttgaag gtacaaatac aaaattatta gatagtagaa
      241 aaacgatacc taatcttaga atattagata agtattctgt taaagtaggt ggaggatgca
      301 atcatagatt taatttatct gatggaatat tgcttaaaga caaccatatt ggggctgctg
      361 gtagtgttaa gaaggcagtt gaattagcta gaaaaaatac ttcttttgtt agaaagatag
      421 aagtggaagt ggaaacttta gacatggtaa aagaagcaat agaagctaat gcagatataa
      481 taatgttgga taatatgagt ttggaaatgg caaaacaagc agtagatatt ataaatggaa
      541 gagccattgt agaattttcc ggaaatgtaa atttagatac tatagaagat ataggaaaaa
      601 ttggtgtgga tgttgtttct gtaggagcgc ttacacattc tgttaaagct ctcgatataa
      661 gtatgaagaa tttgagaaat atataaattt tatattgaaa taaacttaat taaaatagac
      721 tccttaaatt tgttttataa atttaaggag tctatttttt attcattaaa attaataaaa
      781 atagaaaaaa atcaatcaat aatttaataa aaatagataa atactatata cacttttaat
      841 aatatagtta atatctattt tacagataaa taagaataaa cttataatat aattgtatct
      901 gtaaaatttt gtaaaatata aaaagtatag ggggaataaa aatgaaagta gcttatattt
      961 ttagtagtac taatacacat aaaattctag acaaaatgat aataccacaa cttgaacaag
     1021 gaactcatgg agtagacgtt ctagggatgt ttttctttat ggataatacc ttatttttat
     1081 ctaaaggtaa tcctgtaggc gagagactaa gtaaattaca tgaaaaaact gatatgataa
     1141 taatggcttg tgaccaatgt gctatagaaa gaggtataga gaataatttg gttgatggag
     1201 ctacaattgg atgtttccca aacctatatg cttgtttagg cagtgcaggt gtggaccaag
     1261 ttataacgtt ataaattagt ataggataca atccatagaa aaatttattt tctgtggatt
     1321 attttaagta aaagataaat tgtttaaagt aggggattaa caaatgtcta aaattttagt
     1381 tgaatttata aacacttgac catcttgtga tgaacatgga gtgtttataa aaaaaatgtc
     1441 agaaaaatat gctgataaat tggaaataaa attatatcaa gcagggaaag atttttcgta
     1501 tataaaaaaa tatgggatta taacaaaagg aactttaata ataaatcaga aaaagaaata
     1561 tgatagatta aataaagata caattgaaag ggcaatagta gaagctataa acaataatta
     1621 aaagtttaaa aattaggagt atcaaaaatg aatatattag aaattgtttt aaatggagtt
     1681 aaagatacta tagtagcttc tatagttaca ctgaatggag catcaggatg gcttatgata
     1741 agttttatat tggcaggtgt acttcacaat gttttaactc caagaaggtt tcaaaaacaa
     1801 cttggaaata agaaattttc ttctttaatt aaaagtacta tctcaggaat gtttttacca
     1861 atatgtagtt gtggagtaat accacttgga ataagtatgt attattcagg agcatattta
     1921 ggaccagtac ttgcttttat gacatcaact ccaataataa atccaatagc tgtagttctt
     1981 tgtctaggtc ttttaggtcc taaagttaca atcatttatc taatagctgg ttttttagta
     2041 ccatttttag taggattttt aggaaattgt tttggaaaag aagaattatc acttcataat
     2101 gattcagagg aagaagtaat agaattagaa gaagaaaaaa gaagttttat gggaaatatg
     2161 attgatggtc ttaaatggtc ttttggtgaa ttagccctta ctattagtaa atatgtagta
     2221 atgggtatgg tttttgcagg atttataaca actatttttc caaatagtat aattcaaaaa
     2281 tatttaggaa atccaggttt gctatctctt ggaagtatag caatacttgc atgtgttatg
     2341 tatgtatgtg ctgtaggaca tataccgttt atagcagccc ttgttgcaag tggagcatct
     2401 cctggtatag cgataacatt tctaatagct ggagcagcta caaatctacc agaacttata
     2461 agtatgttta aacttattgg aaaaagaact gttattatat attctgtaac tttgacactg
     2521 tcttcaataa taattggata tataacaaat ttacttttag tgaataataa ctcagtaaat
     2581 ttagatgcaa caaattcttc aatacagttg gcaaataaac taatgttaaa tatacctgat
     2641 agttttgagt atgtatgtac tattttaatt attttatttg catttaaatc aataatacca
     2701 aagatgaaag aggtcatagg aaatcatgct taaaaagata gttataatat cctgtatagt
     2761 agttgtgtta atcattttat caaaaatagt tgatgataac atcaaagaag atgcttctat
     2821 tccaaatgtg aataaagaaa cattagaata ttttagaaaa aattataaag aagacataat
     2881 aacttgtgct gaagaagact taaataatga tggaaaaaaa gatttagtag ttatatataa
     2941 aaaaagcaat aattcaaatg aaatggtagt cgtagtttct gataaaaata gccactatat
     3001 aacaaagcca ataccagcac ctatagagaa tcaaacgata acctttaaaa atattgatga
     3061 taaagcacca atagaagtaa ttgtatcagg gtctaaaaat ggtaatgtag gatatgctat
     3121 ttacagagta gagggtaaaa aatttgttga tttatttggc gaagatatgg acaaatgttg
     3181 ctaaaaataa aaataataca taaaggtggg aaatttatat gaaattatct aagaagattc
     3241 aagcattaat cttatttggt gtaatgggta catctatatt aacaggatgt agtagtaaac
     3301 caaaagaaaa agaagaagca aaaactggaa aagatgatta tacaataaca gtaggttatt
     3361 ataattgtga ccatatgact gcgggaccag tagcagaatc ggcaggaata tataaagatt
     3421 taggtcttaa tgtaaaaact gttggaaatg gtaaagttcc tgaagctatg gcagcaggaa
     3481 aaatggatgc aggttacata ggaactaagg ggttagttgg agcaatacct aagggttcgc
     3541 caataacaat agcagctaac aaccatactg gtggttctga atatttaata gtttcaaaag
     3601 atataaaaga acctaaagat ttaataggta aaaaaattgc aacagatatg agtgatttct
     3661 tatggacaag tgattatggt ccagaaacag gtctaccaac tgacccatca aaatatgaag
     3721 ttgtaaatat ggattcagac aaagataaat atttagctct taaaactggg aaaattcaag
     3781 cttttacatc ttgtgaccct tggggttctg tagctgaaaa tgacggtgca ggaaaaataa
     3841 tagcatctac tcaatataaa gaaaaagcta atggcaaaga atataactgt tgttcattct
     3901 cattaaacaa aaactttata aaagaacatc cagatttagc aaaaaaattg gttctagcac
     3961 atacaaaagc aatagaatat atatatacaa atccagctga agcagctaaa atatttgcaa
     4021 aatattataa tgtagaagaa gaagttgcac ttagaactat ctataagaag actgttggag
     4081 aaggtagaac tcttacttgg aaagttacag gtgaagaata taaaaataat ttacaaatgt
     4141 ataaagattt aaaggcttta gatgatatac ctaaatatga agatacgatt gatacaagct
     4201 tattggactc ttgtggagct gatgattttg acaaatttat aaaagaaaaa gttaacccag
     4261 agtttcctga aggtatgagt tatgaagact ggaaagcaaa ggtattataa ataaatggag
     4321 aatatgaaaa atataataat actttttatc attattttgg gaagtatatt tataatattt
     4381 tttaattttg aagataaaga agaaataaaa ggaaaattaa gaataggggt atctgatgat
     4441 acctctggtt ttgtaataaa ttatatggta aatagagact actttgaaaa tataaaagta
     4501 agtgatataa tggatatttt tacaattaat gattgctgag caagtacaat gcagtgggct
     4561 ctgagttcag aggaaataga tatagcaatc atatgtaaag atgctgcaaa acaatatgta
     4621 aatgtaaata gtggatttga aattgttggt actgtagttc aaaattctga tatatttcta
     4681 ttaagtgata ataatcctga aaagatagga gtcattcaaa atagagatta ccagtcagaa
     4741 ttggttaaaa aatactacaa gaatgtggaa gtagctccct tattagggac tgcactagca
     4801 tatggattag aaagtaattt agtagatggt gttgtaatag attgcataaa gtcaattggg
     4861 ttagagggta aaagaaaatc tacaactaaa ttaggagatt atgataccta tgtattagtt
     4921 gtaaataaag agtttaaaaa aagtaaagca tatagtgatt ttataacact ttacaataaa
     4981 agtgttgatg ctttaaagaa taaagctaca tttaagaaag tattaaaaga gtacaggaat
     5041 ataagaaata tagatatgga ggaaataagc aattggaaat taaaatttct aaagataaaa
     5101 gcgatagatt aactaagaaa aaaactgatg gtagtatgga tattttatat aaaataatac
     5161 tattagttat tatattttta gtctggcatt ttgcagcaaa agatataggg agttctttgt
     5221 tacttccaat gccagtagat gtaataaaag gtttcttttt ctgtgtgaca gatgcagaga
     5281 cagtcactaa tctttttata acattgcaga gagtacttaa aggttttatg tatgcattat
     5341 tgtttgggct tccaatagga tttattatgg gattttctaa aacttttgaa agggttttat
     5401 ctccagtagt agattctgta aggcaagttc caataatggc atgggttcct cttactatag
     5461 tatggtttgg tataggagat ggtccaacaa tatttttaat agctttttca ggggttttcc
     5521 ctataatttt aaatactatt caaggagtaa gagcaatatc aaaggattat tataatgcag
     5581 ctagaagtat gggagcatcg ccaattgtga tatttacaaa tgtaattgtt cccgcatctc
     5641 ttccagatat actaactggt tcaaggattg ctataagtac aggatggatg tctgttatct
     5701 gagccgagtt tatagctacg agtgccggtc tcggttactc aatggtacaa gctcaaacat
     5761 taatgcatac agatgtatta attgggctta tgatatttgc agctttaata ggatttataa
     5821 tagatagggt attaaagttt ataaataaaa tcctttgtag atggaggttt gcagattaat
     5881 gaagttaagt gtaagagata taaataagac atttgtaaat aatagagtac atacaaaggt
     5941 tttagaagat ataagtatgg atatagatga tggacaattt gtctgtcttt taggaccatc
     6001 aggttgtgga aaaacaactt tacttactat aataggtggg tttcaaaaat cagaacgtgg
     6061 agatgttttt ataaatgata aaagagttaa aaaaccgggc atagatagag cttttatatt
     6121 tcaaaattat gctttatttc cttggaaaac tataagaggt aatgttctat ttcctatgaa
     6181 acagcagaaa ataccaaaag aaaaaagaga agaaatgcta gaagaacttt tggttatgtc
     6241 agacttgaaa ggaaaagaaa aactgtttcc gcatcaatta tctggaggaa tgaaacaaag
     6301 agtggccatg ataagagctt tagcatgtaa tccagaggta ttactaatgg atgaaccatt
     6361 aggagctgta gattttcaaa tgagacaaaa tttacaggaa gagctagaac gaatatggat
     6421 aaagaagaaa ataactgcat taatggtaac tcatgatgtt gatgaagctg tatatatgag
     6481 tgatagagtt atagtaatgt cgagagataa aggaagaatt attgatgata taaatataga
     6541 tattccaaga cctagaatta gaggaagcca aaaatatgaa gagtacaaaa acaaattaac
     6601 tgatacttta tcaaagtgtt atgaagttta aagaggagta gacatgagtc aaatagaaaa
     6661 atacaatagt ttggataata cacaacagaa aataagaatc tctattatgg atatgataat
     6721 agataaaggt tctagtgtta cacttcaaga agtgactgaa tatgtaagta aaaagttgaa
     6781 tatagaaaaa gaatatatag aaaggacact acaatatttt atttataaaa atataatggt
     6841 tgttgatggg aatagtataa attttatata tcctgtatca gcattgccaa caaatcataa
     6901 agtaactttg agagataata gaagtttcag tgcaatgtgt gctatagatg ctataggaac
     6961 ctcttgcaca ttcaaccaag atataaaaat aaactctatt tgtagtgcta caggtaagga
     7021 aatagaagta attataaaaa atgaaaagat tgagtatgtg aataatccaa acttaagagt
     7081 gcttcatata aatcttgata agcatctaaa ttgggctgct agttgctgaa atataatgaa
     7141 tttcttctgg acgaagagtg attttgatgc ttggacaaac gaagcgggat tatctgatga
     7201 tgaagatata tattgtttag atattaatga ggcaatagta gaatcatata aaatatttaa
     7261 actcaaacaa aaggtactat catagatata catatatgaa aaactaaaaa tgacctttag
     7321 ataaaaatga taggtaatat ccatactata taaattagta tagagtaggg atgtgaaaag
     7381 tatttaaatc taaaggtata tttttagttt ttttagatga ttagatagcc ttattaagta
     7441 tatatttatt tataaccttt gcaactccat tttcattatt agtatcagta acataatctg
     7501 caatttcctt tatactatta cgtgcattcc ccatagccac accaagacct gcatacttta
     7561 tcatatgttg gtcattttct tcatcgccaa cagcaatagc attttctata ggtatattta
     7621 gattcgtaca taatgtttta agagcagttc ctttattagt agttttacct aaaaattcta
     7681 gataaaaagg agcagacctt actatagtat atttattgaa taaattttta ggtagttgct
     7741 gtatgatttc ttccaatctt tctggttcat caactatcat aattttacag attttaatag
     7801 attcatcaat tttatctttt tgtatatatt tgacttctat attatttaat ttcgcttcat
     7861 gaagtgtata tttgccaggc gtttcatttg gtgtaattaa atttgtcatt gtatttatgt
     7921 gtattttagt cttatatttt ttacaaacgt tattatatat aaaatcaaaa tcatctaaac
     7981 tcatttctac atcatgtatg attttctcac ttttaacttc ttgaataatt gctccattaa
     8041 agcatataac ataatcacct tcatctgtta aatttagttg atttagaagt tcttttacac
     8101 caggaagagg tctgcctgtt gttataacta cttttgcccc agcccttttt gcctcctgaa
     8161 tggactcaaa gacttcagga gtaatccttt tttgtgtatt taatattgtt ccatcaatgt
     8221 ctagtgcgat taatttgtac atatcaacct ccaataaatt atatactcaa atttcctgtg
     8281 aaaacgttga cacagctagt gttgaacaaa gtttcagtgg gaaatatggt ttgttttgta
     8341 tactattata tcaagagata agtaatttag aaagtttaat aatattaatt ttttgataaa
     8401 aatattactt atgtgagatg agaatgtaaa aactttgtaa tcaataattc caaaattctt
     8461 aattacaaat aatggtatcg tttgcattgt taatataaat aaatgtatat tatatagtta
     8521 gaagtttgaa taaatagact gatggtaatt tgttatatta gttagttttg tgttataaaa
     8581 tattgtaatt ccattaatca tatgttatta ttcaacatga ttataatata aagaatattt
     8641 gatatatata ataatttata aaagagtatt acatagtaca tataaatcta tatttataaa
     8701 tacaataaag taaagttgta aaggagtgct ttaaatgaag ttttcagagt ttaagtatga
     8761 aagacccaat tataatagta tgaaaaaaga gtttttgagt tgtgtagaag atataaataa
     8821 ttctcgtaat tataaagagc aacaaaaaaa tatacataag attaatttat taagaaataa
     8881 aatagaaacg ttgtcaaata tagcttcgat aagatatagt acagacacat ttaataaatt
     8941 ctacaaagaa gaaaaaaatt attgggatga atatatgcct ttgtatgaag aattaaattc
     9001 atatttttat aatgccattg taaattcaaa atttaaatat gatttgataa aagagtttgg
     9061 agagcaattt ttcacaatag tagagtattc tttaaaaagt ttttccaaag aaataataag
     9121 tgaattgcag gaagaaaata aattgtgttc agagtatact agacttttag cttcagctga
     9181 gataatgttt gatgggaaaa taagaaattt atctggtatg gggaaattca tgtattctaa
     9241 gaatagaaaa acaagagaat tagcaaacaa agcatattat aatttctttg aagaaaatga
     9301 aactaaattt gatgatatat ttgataaatt ggttaaactt agagataaga tgtctaaaaa
     9361 attaggtttt gaagattttg tagagcttgg gtacattaga atgatgagaa gtgactacag
     9421 agaatatatg ataaaaaatg ttagaaaaca agtactaaaa tatgttgttc caatggcaaa
     9481 tgagctttat gaaaaacaag ctaaaagaat tggtctagaa tatttaagtt acatagatga
     9541 aggcgtggaa tttttaacag gaaatgcaag tttaaaggga gattcaagat acataattaa
     9601 aaatggtaaa aggatgtatt ctgaactgtc aaaagaaaca aatgaatttt ttgattttat
     9661 gttagaaaat gaacttatgg atttagaaac gaagaaagga aaaggagctg gagggtattg
     9721 tacttatata ccagattata agtcaccatt cattttttct aattttaatc aaactgctga
     9781 tgatatagat gtattaacac atgaggcagg acatgcattt cagttgtata tgtctagatg
     9841 gattgatatg ccagaaatta attttccaac gcttgatagt tgtgaaatcc attcaatgag
     9901 tatggaattt ataacatggc cgtggatgga tttatttttt aaagaagata cagataagta
     9961 caaattcact cacttatcat cttcaattaa atttatacct tatggagtaa ttgttgatga
    10021 atttcaacac tatatttaca aaaatcctaa tgtagataag tcaaaacgaa aagaaatctg
    10081 gagattttta gagaaaaaat acttacctca tagaaaatat ggtgataatt cttttttaga
    10141 gagaggttgt tggtggttta aacaaggaca tatatttaaa aatccgtttt attatataga
    10201 ttatgtatta gctcaaattt gtgcgttaca attttggaaa aaaatgattc aagatagaga
    10261 tgcaggatgg aaagattata taaatatctg taaagttgga ggaacaaaat catttttaga
    10321 tattgtgagt atgggaaatt tgtattctcc atttgatgat ggctgtattg agtctattat
    10381 aggtgatgtt aaatcttggt ttgatgaaat aaatgacagc aaattataaa aaattatgag
    10441 aattttattg tataaaagta aaaaattaaa aaatatataa aaagtgatta tttgttgtga
    10501 aaatagtctt gtttttaact aaattgataa aataaactaa aaaa
//
LOCUS       DAGSHT010000117        10256 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGSHT010000117 DAGSHT010000000
VERSION     DAGSHT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.120"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..718
                     /locus_tag="MJB72_002532"
     CDS             <1..718
                     /locus_tag="MJB72_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HBY8263616.1"
                     /translation="LENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDG
                     IIFSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKI
                     MKRSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESS
                     NSTLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEV
                     FFGVTIGAIWASVIIKRMIR"
     gene            822..2006
                     /locus_tag="MJB72_002533"
     CDS             822..2006
                     /locus_tag="MJB72_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY8263617.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2213..2926
                     /gene="rpsB"
                     /locus_tag="MJB72_002534"
     CDS             2213..2926
                     /gene="rpsB"
                     /locus_tag="MJB72_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBY8263618.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3005..3916
                     /locus_tag="MJB72_002535"
     CDS             3005..3916
                     /locus_tag="MJB72_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBY8263619.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4001..4705
                     /locus_tag="MJB72_002536"
     CDS             4001..4705
                     /locus_tag="MJB72_002536"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBY8263620.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            4731..5288
                     /gene="frr"
                     /locus_tag="MJB72_002537"
     CDS             4731..5288
                     /gene="frr"
                     /locus_tag="MJB72_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBY8263621.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5291..5494
                     /locus_tag="MJB72_002538"
     CDS             5291..5494
                     /locus_tag="MJB72_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263622.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5523..6257
                     /locus_tag="MJB72_002539"
     CDS             5523..6257
                     /locus_tag="MJB72_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBY8263623.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6270..7049
                     /locus_tag="MJB72_002540"
     CDS             6270..7049
                     /locus_tag="MJB72_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBY8263624.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            7873..9684
                     /locus_tag="MJB72_002541"
     CDS             7873..9684
                     /locus_tag="MJB72_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY8263625.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAVLSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(9772..>10256)
                     /locus_tag="MJB72_002542"
     CDS             complement(9772..>10256)
                     /locus_tag="MJB72_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263626.1"
                     /translation="FVKKDNSNFNYTNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPD
                     GTEIFFTWEEIHCWDEKVATEKTFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKY
                     YEEKGLKIERHALGSEDGEYVELTNDSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK
                     "
BASE COUNT         4136 a         1054 c         1812 g         3254 t
ORIGIN      
        1 cttagaaaac tgtgatttta atagaaccat atttagtaaa cactatataa atgaccgtat
       61 atttgaaaaa gtgaaattta aaggacctga tttggtagtt aataaagtag aaaattttcc
      121 acgaatggat ggaataattt ttagcatgtg tacaaaattt gtgttaaaga atgttgaata
      181 tggaaaaagt gagtatgagc atggcaagat aaattataga atagctagaa atcaagcaac
      241 aaaaattgga gaatatgaga tgataggatt ttactattat aaggaaagaa tttatagtag
      301 taaaataatg aaacgttcta attatcctac tttcagtgat tatttagttg aaaaattttt
      361 tgaccaaata gcacgttaca caacaggtta tggagaaaag ccatggaata tattattagt
      421 tattatagca ataataagtg tttttgcttt attatactta tttgtaggca tagaaagttc
      481 aaatagcaca cttgtagctt tagatataaa taatataggt gattactcac taagtgagat
      541 tttcaaaatg tatatggatt tatggtattt tagcatggct acatttagta cagtaggata
      601 tggagatatg gttgctacaa gtttgattgg gaaagcattg gcaggtatag aagtattttt
      661 tggagttaca ataggagcaa tttgggcatc tgtgattatt aaaagaatga taaggtaata
      721 tttgtgtaaa tatggatagg tattaattac tagatataaa ttggactaaa tagggaaaat
      781 ttaaatataa atatacttaa taatccaaga aggtgaaaaa tttgaaaaat gcactaaaaa
      841 aaattatatc attagccatg attctagttt ttataatacc aaattattca tatgcagatg
      901 ataaatctgt agacaagtat tcaaaaacat ctatacttat agaccaagaa acaggtagag
      961 ttttatatag taaagaacct gatatgaagg taccattagc tagtttaagt aagatgatga
     1021 cttttttact tgcaatagaa gctattgaaa ataaagaagt aaaagaaact gataaagtaa
     1081 aaatagacaa atctattgca tcagtaaaag ggtcaagtta taaacttaaa gatggtgaag
     1141 aagtgccact tatagagctt atgagaggtc ttatgattgt atcaggaaat gatgcggcaa
     1201 ttgctatagc aaaacatatt tgtaaaacga aagaagcatt tgtagataga atgaataaaa
     1261 aagcaaaaga aataggaatg agtaatacac attttctaaa tccaaatggt cttccaatat
     1321 acgatttaag caaccccaaa aaaccagcaa aagaaaatat ctctactgca aaagatatag
     1381 ctattcttgg aaaatacatg tttgaccatt atgaaaaaca agtaacagct ataactgata
     1441 tggagacata tacaaattca gatagaagtt ttgaaaaaag caatacaaat gcattattaa
     1501 gaattatacc agaagtagat ggtataaaaa ctggatatac tggcaatgct ggatattgct
     1561 tatcattttc tatggttgta aataaaaatg ataaaaatga gaaaaaacgt agagtaatag
     1621 gggttgtatt aggtactaac cataaaaata aaagaacctc tgcttcatta gctctactga
     1681 aatatggaaa agaaaatttc aatatgaaaa aagtaattga taaagatagc tttattggta
     1741 agaaatatat aaaaggaatg aaagagttag aggttacatt aaaagcaaaa gatgaacttt
     1801 atacaatatt aaaagatgat gaaagtataa aatcagaagt taagattaaa aatatagagt
     1861 accctgttaa aaaaggtgat actatgggta ttataaaata ttatactaat tcaggtgact
     1921 tacttggaag tgtagatata gtaagtaata atagtataga taacgtatca ttaaaaacaa
     1981 aattaaaaat gaaatttgca aattaaattc aaaaaatggt ataaaataca caaaaaaata
     2041 taaaaataga aaatatggaa aaaatattgt tatttcttca cctctgtgat atactactag
     2101 aggtgaaaaa ataaaataaa tcacacacag cattgggatc cttaaaaggt gcctattaca
     2161 tggtttttta aggagaagat caagctggag gtataaaacc aggaggtaag atatgtcagt
     2221 aatatcaatg aaacaattat tagaagcagg tgttcacttt ggtcaccaaa caagaagatg
     2281 gaaccctaaa atggctaagt atatattcac agagagaaat ggaatatata taatcgactt
     2341 acaaaaaaca gttaaaaaag ttgaagaagc ttacaagttc actaaagaag tagctgaaac
     2401 aggaaagcca atcttattcg taggaactaa aaaacaagct caagatgcga taaaagatga
     2461 agctgaaaga tgtggaatgt actttgttaa tgaaagatgg ttaggtggaa tgttaacaaa
     2521 ccacaaaact ataaaaacaa gaataaataa attaagagaa ttagaaaaaa tggaagaaga
     2581 aggcgtattt aatgttcttc ctaaaaaaga agttataaaa ttaagagctg aaaaagaaaa
     2641 actagaaaaa tacttaggtg gaataaaaga catgcctgaa ttaccaggtg caatgttcgt
     2701 agttgaccca agaaaagaaa acatagctat tcaagaagct catagattag gtataccagt
     2761 agttggtata gttgatacta actgtgaccc agaacaatta gactttgcta taccaggaaa
     2821 tgatgacgct ataagagccg taaaattaat aactggtgct atggcaacag ctgtaattga
     2881 aggtagacaa ggtgctgagg aagaagtagc tgaagaccaa gaataataat ttgacaatta
     2941 aatggtaagg gtcgccctta ccatttataa tatggaatag aataagtagg aggaaactta
     3001 aaaaatggct aatataactg ctcaaatggt aaaagagtta agagaaagta caggcgcagg
     3061 aatgatggac tgcaagaaag ctttacaaga agctgaagga aacatggaaa aagcagtaga
     3121 tttattaaga gaaaaaggat tatcaaaagc tgctaaaaaa gctggtagag ttgctgctga
     3181 aggtctagtt gcaatagaaa tgaatgatga taatacagtt gcttctatgg tagaagttaa
     3241 ctcagagaca gatttcgttg ctaaaaacga agacttcaaa gtgttcgtta aagatgctgc
     3301 ttgtatggca ttagctactg ataaagaaga tatagcttct ttattaggtg aaactcataa
     3361 agagggaata actttacaag aagttttaaa taatagagtt gcaaaaatag gtgagaaatt
     3421 agactttaga agatttgcta aagttgtaac taatggacaa gttgctggat acattcacgg
     3481 tggtggaaaa ataggtgttc ttgttgaaat ggaaacagaa gctagagatg ctaaagtttt
     3541 agaattaggt aaagatgtag ctatgcaagt tgctgctatg aatcctaagt atgtttcaag
     3601 agatgaagta gatgctgaat acatagcaca tgaaacagaa gtattaactc aacaagcttt
     3661 aaatgaagga aaaccagcta atatagttga aaaaatggtt aaaggaagat tagaaaaaga
     3721 attaaaagag gtttgtttat tagagcaaac tttcgttaaa aatccagata taactgttaa
     3781 acaattagtt gctgatgttg ctaaggcagt aggttctgat ataaaagttg ttaaagttgt
     3841 gagattcgaa gttggtgaag gtatacaaaa gagagaagaa aactttgcag aagaagttgc
     3901 taagcaactt aagtaataac caaagagaga acacgcaggt gttctctttt ttaaaaaata
     3961 gaaaaaattg gatatgaagg attgaaatag gaggtttctg atggataaac caatgtacaa
     4021 aagggtattg ttaaaattaa gtggagaagc attagcagga gaaagaggtt ttggtataaa
     4081 taatgatgta gttaatgata ttgctattgc tataaaaaaa atacaagaaa taggtgttga
     4141 agtagctgta gtagtaggtg gaggaaactt ctggagaggc agaaccagtg aaggaatgga
     4201 cagaactact gctgactata taggaatgtt agctacagtt atgaatgcaa tggcgctaca
     4261 ggatgcacta gaaaatatag atgttgctac aagagttcaa actgctatag acatgagaca
     4321 aattgcagaa ccatatataa gaagaagagc agttagacac ttagaaaagg aaagagttgt
     4381 tatatttggt gcaggaacag gaaaccctta ctttacaaca gatacaactg ctgctttacg
     4441 tgctgctgag atggaagcag aagttatatt gttagctaaa aatgttgatg cagtatatga
     4501 caaggatcca aaagtacatg cagatgctaa gaaatttaca gaattaagtt acatggaagt
     4561 tatacagaaa gaattaaaag taatggattc tacagctaca tctttatgta tggataataa
     4621 aattccaata aaggtatttg agcttacaac agagaatata ataagagcag taaaaggcga
     4681 aaatatagga actactgtaa aataatgaag aatgaaggag gagtaaaatt atgaaactgg
     4741 aaatacataa gcaattagaa gagaaaatga atgggacaat tgatgcttta aagtttgaat
     4801 ttggaactat aagagcagga agagctaatg cacaaatgct agacaaaata agagttgatt
     4861 attatggaac tccaactcca ataaatcaaa taggtgctat atctgtacca gaaccaagaa
     4921 tattaatgat atctccttgg gataaatctg ctatgcatga aatagaaaaa gcaatagcta
     4981 actcagactt aggattaaat ccatcaaatg atggtgaggt tataagactt tctgtacctg
     5041 ctttaacaga agaaagaaga aaagaattag ctaaaaaagc tagtaaagct gcagaagaat
     5101 ttaaagttag aataagaaat gaaagaagag acgccaatga gaagattaaa aagatggaaa
     5161 aaggcggaga attaactgaa gatgaattaa aaaaagcaca agatgaagtt caaaaaatga
     5221 ctgataaatt tataaaagaa atagatacat tattatctaa aaaagaaaag gatataatgg
     5281 aggtataata ttgaaaaaat attataacct tttaggtctt catgtagatg aggtaaaaca
     5341 atattttgat gagcaaaata taaattatac cgtaaaatct attgaaggta aaaaggataa
     5401 agaaaaactt gtagttccta gagttataaa aatatctgag ataggtgata gtgtagaact
     5461 gataatcaca tacttttctg actccttagt ttaaggagtc agaacaatat tggaggcaaa
     5521 atatgaataa caacattata tatgatatag acttaaataa catacctact catattgcta
     5581 ttataatgga tggaaatgga agatgggcaa aagctagatt tcttccaaga actgcaggac
     5641 ataaggctgg agtagaaact ataagagata tagtaaaaga atgctcaaaa ttaggtgtta
     5701 agcacttaac attatatgca ttttcaacag aaaactggaa aagaccgaaa cttgaagttg
     5761 acacacttat gaacttatta tctacatact taagaaatga aatagctgaa cttcaccaaa
     5821 acaatgtaaa agtaactgct ataggagata taagtgcttt accaaaaact tgtataagag
     5881 aattaaactc agctaaggaa ctgacaaaaa acaatacagg agttaactta aatttagcat
     5941 taaactacgg aagtagagca gatataaaaa atgctcttat agatatagta aaaaattgtg
     6001 aaagtggtaa aatagatata aacaatattg atgaagatat tataaaaaac tatttaagca
     6061 caaagtctat accagatcca gacttggtaa taagaacaag tggtgaacaa agattgagta
     6121 actttttact ttgggaagtt gcttattctg agttttactt tactgatata cattggcctg
     6181 attttaaaaa agaagaacta cagaaggcta tatacgtata tcaaagaagg gatagaagat
     6241 ttggaggact taagtaagga gattatttta tgcttacaag aattattgct tcactagctc
     6301 tggttccatt atttctattt gttgtatatg gaggtattcc tctatatata gctgagacag
     6361 caatagttta tattgcattg catgagtttt ataaggcatt taaaataaaa gatgtacatc
     6421 caatattcat tataggatat ttattttcta tttatttagc tgtaaaaaat atttttaatt
     6481 taccattaga atatacttat gcagtaattt ttattttatt tttagcaagt ataatttata
     6541 tgttgatggg caaaaacaat gttatagatg tttcgattac gtttttagga gttttttata
     6601 taggagtatt tttagatttt ataattataa ctataaatgg atttgaaaaa ggtagtatat
     6661 atgtatggct tatatttgtg atatctttta tgacagatat atttgcatat tttagtggtt
     6721 atttacttgg aaaacataag ctaattccta aagttagtcc taaaaagact atagaaggag
     6781 ctattggagg aataatagga agtacacttt gttgtatttt atttggatat ttatttggca
     6841 ttgatttgct tcaattagcg ataataggaa gtataggaag tgtaatagct caattaggag
     6901 atttatttgc atcttctata aagagatatg tagggataaa ggattatgga aagataatac
     6961 ctggtcatgg gggtatatta gatagatttg atagtgttat attagttgca ccttttgtat
     7021 atagtgcgat taaatttttt attagataaa gattggttgg atttaaaaga gcaatctata
     7081 attgctagta atgaaaatag ctatgtaact tttaaatgca gagatattct gcagttatta
     7141 gttacatggc tattttcatt gttcaaaaat ttagaagacc ttggggaatt gtataagaat
     7201 aggtattact ttataatagt atactatatt tgtagtgagt atatttttta tactataact
     7261 atcattaaaa aatatttttt tgtttaataa tttcgatatt tttagctata ttatatattt
     7321 ataatatata tgtataaatg taactaattt attatccatt tataaatttt atttttaaat
     7381 ataattaata agaaataagt aaattattgg tcttattaca caaaaataga tataatttaa
     7441 gtaatatacc aaaaaaaagg aatatattta cataatatga taaaataaat tcaaagagtt
     7501 aaattgtagg taaatagtat tttaattatt tattaaaata tagattatgt atatgccaaa
     7561 tttaaaaaga gacataaaga cattatacaa atatattatt ggggtgatat tttataagaa
     7621 aagaagtgat gcagtaaatt aattgagcaa tttaaaaatg atttaatgat agtgttgatg
     7681 aatgtttaaa aagaaatttt agaaaaacgt atattgctgt ttgtgttgga ggtgatgaat
     7741 ttgtataatt tataggatat aaatgattaa actatttaag ataaatacat atcactgtaa
     7801 aatattgata atcatttttt ataagagatt gattatttgc aaattatata tattacaaat
     7861 gaggtaacaa taatgaagaa aaaatatgat tttttaattt attctgctat ttggctagtt
     7921 attattattc tttttatatt ttctctatat catagtatag agcatattaa aattataagt
     7981 cacactggta ctattcgcag tgaaactcaa aaggttgtta agcaagaatt gaataatgaa
     8041 agaaatgatg acttgataaa acgactggat aatattctga taaagctaag aacaggtaat
     8101 ggagaaaatg gatttcaaag atgtgataat aaagaatttc aacaaaaact aaatcaaatg
     8161 gattctatgt gggaatctat gaagaaagaa attataaaag ttagaaatgg tgcttcaggt
     8221 gataaattat ataaattgag tgaagagtat tctgtgttgt ctaatcaaat tgtttttata
     8281 tctgaaaaac actctaatgc aaaattgtat tcttttgcta ctgcactatt tatttatctt
     8341 atattctcaa caataagtct tttaatttgg gaatattata ataaaaaaag gtttaaaaga
     8401 atattttata ctgataattt aacaaaaata aaaaatcagg ttgcatttga aaacagagcg
     8461 attgaaattt tgcacaatgc atctaacaaa gagtatgtac tgctaaatat tgatattgac
     8521 aattttaaat atattaatga tacacatgga tatgaatatg gagataaagt tctaataata
     8581 gttgcagctg tactttctaa aacttttaat atcaaagaaa cttgtgcaag aattggttca
     8641 gataattttg taatacttgc aaaatataga gattctcttt tagaggatat tcgggaaatg
     8701 ctaactaatg ctataatctc tgaattagat atgaatgtaa ctcaaactat atcttattgt
     8761 ataggagctt acttagttga aattgataat ttaggttata aatctatcaa ttctatgatg
     8821 gataaggcaa atattgcaca caaggtaagt aaaacaagag gaatttcctc cacagtatgg
     8881 tataatgaaa atctgttaaa acagttacaa atggaaaata gtatttataa ttatatgtat
     8941 aaagcgatag aaaatgaaga atttcatatg tatttgcaac ctaaatttca aatatcttct
     9001 ttaaatgttg tgagcgcaga agctcttgta agatggtttt ctccagaact tggtttttta
     9061 tcaccagatg aatttattcc attgtttgaa aaaagtggat ttattattga attggacttt
     9121 tatatgttaa aaaaagcgtg ttcatttgta aaaaaaacgt ttatgaaaaa aaatcaatat
     9181 acatatccga ttgcagttaa tttttcaagg gttactattt accaaaatag tttttatcaa
     9241 aggtttttag atactgttaa agaatatgaa attcctttta agtatataga gatagaggta
     9301 actgaaagtg cttttaatga aatatctcaa ccagttattt cgattttaga ggagcttaaa
     9361 aagttgggat ttttaatttc tatggatgat tttggatcag gatactcttc attaagtctt
     9421 ttatgttctc tatctattaa tggattgaag ttagataaaa gcttacttaa agaaacattt
     9481 aaccgggaga aagtatatag tattattcag tgtattattg aaatgtcaca tcgtataggc
     9541 atgtctgttg tatgtgaagg tattgaaact aaaaaagact tggaattttt aaatacagtt
     9601 aaatgtgatg taggacaagg gttttatttt tctaaaccga tagaagaaaa agaatttttt
     9661 aacaaatatg taactaaaaa ataatataat tatttttatc agtctataca ttgagtcatt
     9721 ttatatctcg ttaaatgact caatgcactg ttatgtattt taaaatataa attatttaaa
     9781 acgtttttta tacttatata gaaattcttc tatatttttt gattgttctc ttatttcaac
     9841 tgcttctata tctagtaaat aaagtataga atcatttgta agttcaacat attcaccatc
     9901 ttctgaaccg agtgcatgtc tctcaatttt taatcctttt tcttcataat atttttttgt
     9961 ttctatatat aatgattctc gcttttgtat gtagtctttc acttcattat ttggaaagtt
    10021 atatgaatcc actaatgttt cgccatttat aaaagttttc tcggtagcaa ctttttcatc
    10081 ccaacaatgg atttcttccc aagtaaaaaa tatctcagtt ccatctggaa tgaatgaaaa
    10141 cctggtaata atatataaat aattattgaa atttacttca taatattcag gaatatcttc
    10201 aaattccata tagtaatttg tataattaaa attactatta tcttttttta caaagg
//
LOCUS       DAGSHT010000118        10194 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGSHT010000118 DAGSHT010000000
VERSION     DAGSHT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.122"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(139..1389)
                     /locus_tag="MJB72_002543"
     CDS             complement(139..1389)
                     /locus_tag="MJB72_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBY8263627.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1421..2362)
                     /locus_tag="MJB72_002544"
     CDS             complement(1421..2362)
                     /locus_tag="MJB72_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBY8263628.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2602..3678)
                     /locus_tag="MJB72_002545"
     CDS             complement(2602..3678)
                     /locus_tag="MJB72_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY8263629.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4051..4566)
                     /locus_tag="MJB72_002546"
     CDS             complement(4051..4566)
                     /locus_tag="MJB72_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBY8263630.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4929..5999)
                     /gene="leuB"
                     /locus_tag="MJB72_002547"
     CDS             complement(4929..5999)
                     /gene="leuB"
                     /locus_tag="MJB72_002547"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBY8263631.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6006..6497)
                     /gene="leuD"
                     /locus_tag="MJB72_002548"
     CDS             complement(6006..6497)
                     /gene="leuD"
                     /locus_tag="MJB72_002548"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBY8263632.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6512..7789)
                     /gene="leuC"
                     /locus_tag="MJB72_002549"
     CDS             complement(6512..7789)
                     /gene="leuC"
                     /locus_tag="MJB72_002549"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBY8263633.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIKDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7746..9488)
                     /gene="leuA"
                     /locus_tag="MJB72_002550"
     CDS             complement(7746..9488)
                     /gene="leuA"
                     /locus_tag="MJB72_002550"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBY8263634.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RSHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDFNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9612..9858)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10006..>10194)
                     /locus_tag="MJB72_002551"
     CDS             complement(10006..>10194)
                     /locus_tag="MJB72_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263635.1"
                     /translation="SIKSKQSQKFNYKIIEKIEVVKNILNIYTNESNYKIRLRALSLE
                     DEKKLLNIIDEKMKKFKA"
BASE COUNT         3060 a         1978 c         1061 g         4095 t
ORIGIN      
        1 cctaaaatta tccatttcta aaaaaaaggt aactattatt aagaatatca acttatagaa
       61 tttatattcc acataagatt ttcaatcaaa aaaacaaggg ctaaaaagtg aattgtgcac
      121 tttttagccc tttattaatt attcatttat ttcatgtaag aatagaccac atcttacttt
      181 aggttcaaac catgttgact tagctggcat tatcttattt gcatctgcta ctcttattaa
      241 atcttcaata ctagtaggat acattgcaaa tgctactttc atatcttcat taactctttt
      301 ttctaattct tcaagacctc taattccacc gataaaatca attcttttat ctactctagg
      361 attatcaatt cctaaaatag catcaataac atagtcttgt aatatagaaa tgtctaatga
      421 gtcaacaggg tcttctattt ctaataaatt tttatttgct ttcattttat accacttatc
      481 acctaggtac attccgaaag ttcctttttt gtcaggttta tatttttctt ttccttctat
      541 ttcttctagt tcaaaatttt cttttatttt atttataaat tcttcctcgc ttaaaccatt
      601 taagtctttg ataaccctat tataatccaa aaccattaaa ttctcatctg gtgcaatcat
      661 tgctatgaag taattaaatt cttcttttcc agtataattt ggatttttag ctctcatttc
      721 tttggctaca ttttctgctg aagctgtcct atgatgacca tctgcaatgt atagataatc
      781 taatttttca aatttatcta ctaggttatc tattatatca tcattatcaa tcttccatac
      841 tgtatgctct atcttgtcat ctgttatgaa atcatataaa ggttcattat tatccataaa
      901 atcattgatt atatcatcta tcaccctttg atgtttgtaa gttacaagta tagttcccgt
      961 attagcatca cagtatttta tgtgtttagt tctatcaatt tccttttctg gtcgagtgta
     1021 ctcatgtttt tttattgttc cattcaagct ttcatcaact gatatacatg ctactatacc
     1081 aacttgagct ctaccatcca tgatttgttt atatatatat atacactctt tgtcatcttt
     1141 tactagaatt tcttgttttc gaaactcatc taaattttct cgcgctttta aataaacttt
     1201 ttcatcatat aaatctatat tttcatctaa atcaatttct gatttatcta tatgcaaaaa
     1261 tgaatatgga ttcccttctg caattcttct agcttcttta ctattcataa catcgtaagg
     1321 aagtgctgct actttatcta ctatatattt atttggtcta atcgccttaa atggtttaat
     1381 cgttgccatt actaatttcc tcctaattat acaaaatatt ttataatgct acccctatta
     1441 gattgttgaa gtcaagaaag aaatcttgaa tcacatttac tatttcgtct cctattcttt
     1501 cttgagcttc tactgtagat gccccaatat gaggtgtagg agaaactctt ggatgattta
     1561 ctaagtctaa atttaatgta ggttcttgtt cataaacatc tatagcagct gctgataatt
     1621 taccttcatc taatgctttt agtaaatctt tttcatcaac taaaccgcct cttgcacagt
     1681 ttattaaata agcccctttc ttcatcttat ttatttcttt ttcagttatt actgccccct
     1741 tattcttatc aaatggtatg tgtaaagata agaaatctgc attttctaaa acttcttcca
     1801 tatcacagaa tttataattg ttaaagcctt gcgcctcacc cattatgtca gtatatataa
     1861 cattcatacc taataattct gctctctttg caacttcttt tgcaattctt ccaaaaccta
     1921 taagtccaag tgtctttcca ttaatttctg tacctttata ctttttcttc tcccattttc
     1981 catctctcat agttacattt gcagtattta tgaatcttgc aagaacaaat aattgaccta
     2041 ttgtaagctc tgcaacagat atactgcttg catttggagt attcataact tttatgccat
     2101 tagcttgagc atatttaaca tctatattat ccagacctac tccacctctt actatcaact
     2161 tcaacttttt accctctaat gctttatcta ttacatcttt agtaacttta gtagctgaac
     2221 gtactacaag tacatcaaca tctttaagtt tatctcctaa aacatctttt tcataaaact
     2281 gctctatcac attaaaatct aactctctca attttctcgc tgcttctttc tctattccat
     2341 cagtaactaa tatattatac ataacaaact cctccaattt ttattcactt aatttttcca
     2401 atttttattt tttaaagtat tttcatcaat aaaaaacttt acttaattca actttctgtt
     2461 tatataattt tcaaaatatt tttttattaa agtcatatca aatttttcta ttgtttgtta
     2521 aaaatttaat atgtaatttt tataaaatat tatttattat atattctaaa tttatcttaa
     2581 aacatacata tttttacaat attatttatt aaaacctaat atatcatcaa tattatctaa
     2641 taattcttgt acatcttcga cagtatagtc acccatatgt gaaattctaa aagttttttc
     2701 ttttaaatct ccatagccat ttgctatttg gaatcctcgt tcttgtaatt ttgaatttaa
     2761 atttgataca ctaatacctt gagtattttc tataacagta agtgtattgg ataagtgatt
     2821 ttcattggta aaaatttgga agtgtttttt agcccagttt ctaactaagt ttgccatgtc
     2881 ttcatgtctg ttgaatctgt tatctaggcc ttcatctaat atattatcta attggaaatc
     2941 taaagcgaac atgtgagaaa gagatggtgt tgaagggtat tggtagtttt tcttgattaa
     3001 gtattcatac attgctaaca aatcaagata tactcctcta aaaggtactt gcttagctct
     3061 atcgattgct ttttgagaaa atgtgcaaat agacattcct ggtggtaatc ccagtgcttt
     3121 ttgtactgat gttatacaaa tatctattcc aatcttatca acctcgactt ttattccacc
     3181 ggctgaactt acagtatcta cacagtagat tacatcttca tactttttaa caacttctcc
     3241 aatttcttct attggatttc ttattccagt tgaagtttca ttgtgagtta cagttattaa
     3301 atcgtattct ccggtagaaa gcaccttatc aaccatttct ggcgttgttg cctctcctaa
     3361 ttcaacttta aatatatccg ctgggacatt attagccact gccattttgt accatctatc
     3421 accaaaggat ccacaggaaa atactgctgc ctttttggat gtacaggaac gaatggagcc
     3481 ttccattaaa ccagttccgg atgacgtgga taaaagtatc gtgttgtttg tatagaatag
     3541 tttttgcatc ttttcactta tactcttttg gagcatagat gcatcttttc ctctatgtcc
     3601 tatcattggt gtagccatct tttgcaagac ttcttcttta acatcaatag gaccaggaat
     3661 aaataatttc ttactcatta taattccccc ctttttagtt tgtaatatgg atagcgtttt
     3721 tactgtcctt atttttaata actataatat caagctgaat taactatgtc aacataaaat
     3781 ttatttttta tatataattt attttatata ttttatgaaa acattcattt tctacctttt
     3841 cataagtctg aattttctga attattccct caaaaaaaat atacttttta tctatataaa
     3901 ttaatttttt tagtatgtaa atttgacgct attacgataa atctttttag tataactaat
     3961 ttatttttat cttgtatttt tattgtattt ttaaatttta ttttttctaa atataaaatt
     4021 tatgttttaa aatgtaaaat ttttatttaa tcaaagttca atatattcca ctttttcttt
     4081 cgattcttca aaaaaccatt cttcattaga aaaatcacct tctgcaattt gtccaatagg
     4141 cattttcata tactttccag ttgctgtcac tgctatatct ccatttggta gaattatttc
     4201 tcctgtacct tcaaataatt ttctgttttc tttagttatt cttccaacaa cttttatagt
     4261 agcatctgtt ggtactggtt tcttatattt caactctaaa gatactgtta ctccccatat
     4321 ttggtcatca tttatactta cagctcttcc aatagtctca tccaatattg cagcagacat
     4381 acctccatga actctcccag gataactttg atgccaatcc tttgtattac aaattgatac
     4441 aagctctcca ttttcaagtt cataaaaatc tgctttaagt cctagctcat tttgtgttcc
     4501 acaaactaaa caagatttac tactattttg cttcttcaaa actttatact ttgccaaaaa
     4561 atccatctcc taaccgacat tagtagtcgc ttaatattaa ataattctaa aatatattat
     4621 ataatattaa taaaatccct gcaaacttct aataataaaa ataggctact tcaatatgat
     4681 aagtttaatt taaagtatta ttttccaaaa aataacttta tcaaaataga aactcaaagt
     4741 agatataagc ctctttttcg ttattaaaat agtaaaaata tattttactt aatataaaaa
     4801 agagtgtctc attaactaat aaagtttatt ttgagacacc cctcataaaa caaattatac
     4861 tacaatagat ttttactaat tctacaagtt atatattcaa attatattat tcaaattaaa
     4921 ctataattct aaatttcatt tactattaat tcgcccattg ctcttgttcc tacaacattt
     4981 cccacaccat tatagatatc aatagttcta taacctttat tgagtacact ttgtacagca
     5041 tcttcaatac acttagcttc ttcttctaag ttaaaactat gtcttaacat catgcttaca
     5101 gatagaatag tcgcaattgg atttacaata tcttttccag ctatatcagg tgcacttcca
     5161 tgtattggtt catacatacc aaaactatct tctctaagtg atgctgatgg taacatacct
     5221 atagaacccg taatcataga tgcttcatca gatattatat ctccaaatat attattagtc
     5281 actattacat caaattggct agggtctttt acaacttgca tagcagcatt atcaacatac
     5341 atatgtgaca gttctacatc ttcaaactct ttagctaagt cttccattat acttctccaa
     5401 agtcttgaac tctcaagcac atttgcctta tctacactta taagtttttt atttctattt
     5461 ctggctattt caaaagctct cttaccaatt cttcttattt cattttcgtt atatatttct
     5521 acatcataag cattttcgat accatcaatg atttttcttc cacgttctcc aaaataaatg
     5581 ccccctgtta attcccttac aacaacaaaa tcaatgcctt tctcaactat atccgttcta
     5641 agtggtgaag cttcttttat actttcatac attgtagctg gtcttaaatt tgcaaataaa
     5701 cccaatgact ctcttaattt taaaagacca gactctggtc ttttatctcc cttacattta
     5761 tcccactttg gtcctccaac agcaccaagc aatacagctt cgttttttcg acaagtttca
     5821 acagttttat taggtagagg ttctccagtt tcatcaatcg cgcatcctcc agctaataca
     5881 tattcacaat cgaatctatg attaaattta caacaaattt tatttagaac ctttatacct
     5941 tcatctataa tttccggtcc aacaccatca ccttttatga ctgctatatt acaattcata
     6001 ctccactacc ccctatttgc ttttattgag ttaattaagc cttcatttaa tataatattt
     6061 tgcataaact caggaaatgg ttctgcttta tattcttttc cttttgttat attttttatc
     6121 acacctgtag aaaaatctac ttctatatta tccccttcat ctatgttatt ggcagcttct
     6181 tcacattcaa gtataggaag ccctatattt atagagtttc taaaaaatat tctagcaaaa
     6241 gtagaagcaa tcacacagga tactccactt tcttttatta caattggtgc atgttctctt
     6301 gatgaaccac aaccaaagtt tttagttgca actataatat ctcctttttt taccttagag
     6361 ataaatttat catcaatatc ctccatacaa tgcgttgcta gttctttata atcagctata
     6421 tttaaatatc tagctggtat tattacatct gtatctatat tatcaccgaa tttaaaaaca
     6481 cttccattgg caatcataaa ttttacctcc cttaaattat ttctgatggt tttgatattt
     6541 ttccagttat agctgatgct gctgctacag ctgggcttgc aagatacacc tctgaatcaa
     6601 catgccccat tcttcctacg aaattcctat ttgttgttga aatggatttt tctcctgctg
     6661 caagtatacc catatgtccc cctagacatg gaccacaagt tggtgtagaa actgctgctc
     6721 ctgcctctat aaaagtggta atataccctt tttctatagc ttcaagatat actttttgag
     6781 ttccaggaaa tatcataact ctaactccat ctgctacttt tttgcctttc attatctcag
     6841 ctactacttc aaggtctgat attcttccat ttgtacaaga gcctattaca acttggtcta
     6901 tctttatatc cttatctatc tcatctactg ttctagtatt ttcaggaaga tgaggaaagg
     6961 ctactgtttc ttttagttca gctaaatcta tttcgtatac ttcatcatag actgcatctt
     7021 catctgcttc atatatattt acatcttttg ttatagttga acacttatgt gaatttatat
     7081 attccatagt cttatcgtct actggaaaaa ttccattttt tgctcctgct tctattgcca
     7141 tattacaaat agtaaatcta tcatccatag ataagtattc tactccatct ccacaaaatt
     7201 ccatcgactt gtaaagtgct ccatcaacac ctatctcccc tattatatga agtattatat
     7261 ctttaccact tatccattta tttggtttat ttttcaaaac aaattttatt gctccaggaa
     7321 ccttaaacca acattttcct gtagccatac ctgctcccat atcagttgaa cctacaccag
     7381 tagaaaatgc tcctaatgct ccatatgtgc aagtatgaga gtcagcacct ataattacat
     7441 ctccagcagt tactatacct ttttcaggaa gaagaacatg ctctattccc atatctccta
     7501 catcaaaaaa atttacaact ccttttgatt ttgcgaaact tcttgtaaat ttacattgtt
     7561 ctgcactctt tatatcttta ttaggtgtaa agtggtctaa aaccatcact actttagtct
     7621 tatcataaac atctttaatt cctaattttt caaattctct aatggctact ggtgatgtta
     7681 tatcattacc taaaactaca tctaaatctg cttcgattaa ttgccctttc ttaacttcat
     7741 ttaatttagc atgtttggct aatattttct gcgtcatagt catccccatt ttttatttcc
     7801 tcctcaatat aacttttatt tattgcattc ataagtgcat taatagctgc tgtatttatg
     7861 ttttccgata tacttacacc aaaatagact tcattattgt gctctattcc tacataagct
     7921 acccctcttg aatgtgaccc ttcctctaat gcatgttccc agtaatatct aaatttataa
     7981 tctttcatgc cacattgctt tagtgcatta ttaaatgaat ctacaggtcc atttcctata
     8041 ccaacaatcc tttgttcatt tcccatataa cttattcttg cagtcatctc aatcatgtta
     8101 gtatcattaa aatcatcatt ttcatagttt aattcatcca ttgattttat tttatacttc
     8161 ttaactttat aagggctctc aacttcaata tattctttct taaacaaatt aaatattgct
     8221 tcactactaa gttcttcccc aattctatca gattccattt tgactacatt tccaaaatca
     8281 gaatgcatat tcttaggtat cataaatcca taatctgttt ccattatata aacagctcct
     8341 ccttttccag attggctatt tatccttata atttctttgt attctcttcc aatatcatga
     8401 gggtctattg gtaaatatgg aacttcccag taatcattat ctctgttttt cattgctatc
     8461 atgccttttc taatagcatc ttgatgtgaa cctgaaaaag cacaatgtac aagattacct
     8521 gcatatggat gtctatcatg taccataagt tttgtacagt cttcatatat acctattatc
     8581 ttatctatat tgctaaaatc tagctctggg tctattcctt gtgtataaag attaagtccc
     8641 acatttacta tatccatatt cccagttctt tcaccatttc caaatagtgt tccttcaagt
     8701 ctatcggcac cagccaataa tccaagttca cttgcagccg tacaagttcc tctatcattg
     8761 tgagtatgca aacttagtat aatagaatcc ctacaactta tatttttaca aaaccattct
     8821 atttggtctg catatacgtt tggtgttgcc atttcaactg ttgatggaag atttattatt
     8881 actttttttt gaggagttgg tttccaaaca tctatcactg cctcacatat ttcaagtgca
     8941 taatccatct ctgttcctgt aaaactctct ggtgaatatt caaaagtaaa ttctgtttct
     9001 ggatattttt ttacttcttc attaaataat tttgctcctt caactgctat atctattatt
     9061 tcttgtttac tcatattaaa aaccacatct ctttgaagaa cagatgtaga gttatataaa
     9121 tgtataattg cttttttaca tccccttatt gattcaaatg ttttttctat aagatgagac
     9181 ctcgattgtg taagtacttg tatagttaca tcatctggaa tcatattctc atctattaat
     9241 tttcttaaaa atgtatactc tgtatttgat gctgatggaa atcctacctc tatttcttta
     9301 aatcctgtat caataagcat tttgaacatt ctgacttttt catttacact cataggtgtt
     9361 ggcagtgact gatttccgtc tcttaaatcg acactacacc aaattggagc cttaaatatt
     9421 tcattatctg gccatttacg atttactatt tgcattttat catatttttt atactttcca
     9481 cacttcatgt tatttccccc tttttctgtt ttttgtattc agtatacctc atatcgacgc
     9541 aatcctgcac aattcataag gataaatgtc aattttatca ttagaataaa aaaaacctca
     9601 tctctaataa aattagagac gaggttttgc tcgcggtacc actctattta gctttctaaa
     9661 aagctccctt agtaatagcc aatacaataa ttataatgac tatatttcct ataacgtgga
     9721 attactccga ttattctaag atttaaatct tagaaaacag ctcctagaca agttcagaac
     9781 tatcaactac taaatcacac catcctttag ctctctgaaa gtttcattgt tcttactact
     9841 tctattcatc acttttttaa tcatttaaat tttctaaata ttttgattta atagttatta
     9901 tatacacatt aaaatgtatt gtcaacacaa aaaataatta tctcaatttt ttatttttga
     9961 taagcaattc tataaaacat gctaactatt gaaaacagta tgatattatg ccttaaactt
    10021 tttcatcttt tcatctatga tattcaataa ttttttttca tcttccaaac tcaatgccct
    10081 taatctaatt ttataattag attcgttagt gtatatattt aatatatttt ttactacttc
    10141 tattttttct attatcttat aattaaactt ttgactttgt ttagatttta tact
//
LOCUS       DAGSHT010000119        10163 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGSHT010000119 DAGSHT010000000
VERSION     DAGSHT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.123"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..2884
                     /locus_tag="MJB72_002552"
     CDS             <1..2884
                     /locus_tag="MJB72_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8263636.1"
                     /translation="KHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCDDYGII
                     SSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQKNDER
                     NKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFEKYREE
                     NNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFATAKDK
                     PNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPKLTMVY
                     DSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKSGKATM
                     LCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSIDSYIEK
                     SFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVLDTSYG
                     MRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEYSIQII
                     SFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAKNNPEE
                     YALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFVLLIKY
                     KTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRANTARK
                     TIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDYQIIGA
                     EALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQKVVPIS
                     VNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNNLKEKG
                     FVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMAKELKM
                     KVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3205..3417
                     /locus_tag="MJB72_002553"
     CDS             3205..3417
                     /locus_tag="MJB72_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY8263637.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3430..3651
                     /locus_tag="MJB72_002554"
     CDS             3430..3651
                     /locus_tag="MJB72_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY8263638.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3751..5907
                     /gene="feoB"
                     /locus_tag="MJB72_002555"
     CDS             3751..5907
                     /gene="feoB"
                     /locus_tag="MJB72_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY8263639.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            5933..6085
                     /locus_tag="MJB72_002556"
     CDS             5933..6085
                     /locus_tag="MJB72_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBY8263640.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6265..6975
                     /locus_tag="MJB72_002557"
     CDS             6265..6975
                     /locus_tag="MJB72_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBY8263641.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7060..7830
                     /locus_tag="MJB72_002558"
     CDS             7060..7830
                     /locus_tag="MJB72_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263642.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            7823..8554
                     /locus_tag="MJB72_002559"
     CDS             7823..8554
                     /locus_tag="MJB72_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263643.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8588..9547
                     /locus_tag="MJB72_002560"
     CDS             8588..9547
                     /locus_tag="MJB72_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8263644.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9645..>10163)
                     /locus_tag="MJB72_002561"
     CDS             complement(9645..>10163)
                     /locus_tag="MJB72_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBY8263645.1"
                     /translation="GPVFLGSKPAEIINISFTDVNREEKINDIYKYLYKCKKIDFIVI
                     DKEKKGLKILFVNKKALSEKLKCKKTVNFLKFLGYKQNTNVNAYLEHLVDKLKSDVFP
                     DEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWKVYGDTKQSEDTYSKFLLHRKKMRKLL
                     DYISVDKIVSCF"
BASE COUNT         3891 a         1068 c         1859 g         3345 t
ORIGIN      
        1 aaagcacttc ttgatacttt ctttgatatt tttacttatt atatcatgtt ttaacaatat
       61 actatgcttt gctgatgagt ctgaaaattc aaaaaaaata agagttggat attgtgatga
      121 ctacggaata atatcctcat caaaagagca ttcttataca ggatatggtt atgattatct
      181 aagagaaatc tcaaaatata caagatggga atatgaattt gtaaaagggt cttgggaaga
      241 atgtttagac agactagaaa agggtgaaat agacttactc ggacctttac agaagaatga
      301 tgaaagaaat aaactattta attttcctaa actgagttca ggatatgaat atagtgcact
      361 atatacaaag gatagtaaca ataatatgtt ttatgaagat ataagttcct ttaagggaat
      421 gagagtagct gtacttagag gaaactttca taatactgca tttgagaagt atagagaaga
      481 aaataatttt tcagttgaat atatatactg taattctatt gatgaattaa ttgagtccgt
      541 caatgaaaaa aaggcagatg catttgtttg tggaagtatt ataaatgcta aaggaatgaa
      601 aatagttagc aaattttcag tagaaccttt ttattttgct actgcaaaag ataaacctaa
      661 tttggtaaaa gaattagatt atgctttaaa agaattaaaa ataaatgata tgtattatga
      721 gttggaatta tataaaaaat atttcaaaag agaggtcaat aattccatag catttactcg
      781 gcaagaaatg aattttatta aagcaaaccc aaaacttact atggtatatg attcagagtg
      841 gtctcctgta gagtattatg ataaagaaag caattcattt aaaggtataa gttccgattt
      901 aatgtcaatt atatcaaaaa aatgtggtat aaaatttgaa tatattaaaa caaaaaatta
      961 taatgagagt cttgattaca taaaaagtgg taaggcaact atgctatgtg gctcaatcaa
     1021 tgaaaatgat aaggcaaaac gatttaatat gaaattgaca aatccataca taaatattcc
     1081 aatgattatg gtaggaaaat tggataccaa tctcaataat gatttaaata ttgctttaac
     1141 tagtagttat aagtctattg attcttatat agaaaagagt tttgaaaatg ctaagacaac
     1201 atcttataag agtgtagaaa gttgtctaaa cgctataaat gaaggtaaag caaacttaat
     1261 gatacttagt tcttatcagt ttgatgaact tttaagagaa ggaaagagtg agaatttatc
     1321 tgttatatct gttttagata caagctatgg gatgcgtata ggggtatcaa ataaaactga
     1381 ccctatatta gtgtctattt taaataaatc aatagacaaa attactgaag aagaattaag
     1441 tgattgtata tattctaata ctattgacaa accttataaa gtaccatttg gtgtgatttt
     1501 taaagaatat tcaatacaaa ttatttcttt tgtatgtatt ttattcttaa ttgctattaa
     1561 atatataatt tataataaaa agaagaaaga ggattatcta aggaagatag catatacaga
     1621 cccattgaca ggagcagata gtatagataa atttaaaata aattctaata aactgtttgc
     1681 taagaataat ccagaagaat atgcgttgtt ttatatagat gttgacaaat ttaaatatat
     1741 aaatgatatg tttggatatg atatgggaaa tgatactttg atacatatat caaatactat
     1801 tgcaagtgaa ttaaaggaag atgaaatttt tgcaagagtt tctgctgacc attttgttct
     1861 tcttattaag tataagacag atgatgatat taaaacaagg ttaaacaata tttacaataa
     1921 ggttcaaata cttagtaatc ctaaaataaa ctattataaa ttgattttag attgtggtat
     1981 atataaaata agtaaaagtg ataatgacat aaatactatt atggatagag ctaatactgc
     2041 aagaaaaact ataaaaggtg gtcataaaaa ttcatttgct ttttacgata aggaaatgca
     2101 caaaaaaata ttaaaagaga aagaaattga aaattcaatg gtagatgctc ttaataatgg
     2161 agaatttatt gtttattttc aacctaaata tagcctttca gattatcaaa ttataggggc
     2221 agaggctctt gttcgttggg ataatccaca aaaaggtcta ataccaccaa tagaatttat
     2281 tcctgtattt gagaggaatg gttttattgt aaatattgat ttctatgtat ttgaggaagt
     2341 gtgtaaaaag attagagaat ggatggatga aggacaaaaa gtagtaccta tatctgtaaa
     2401 cttgtctcga atgcattttg taaatagtaa ttttatagag gagtttaaat taattgtaga
     2461 taaatacaaa attccgacaa gacttataga attagagcta acagaaacag cagtattaga
     2521 taatatagaa ggattacttg atacaatgaa caatttgaaa gaaaaggggt ttgtaatctc
     2581 tatggacgat tttggtacag gatattcctc tcttaatctt ttaaaagaat tgcctgttga
     2641 tatattaaaa ctagatagag cattttttac agaaaaagat gagagtaata atgaaaaaat
     2701 tgttatatct aatgtcatta aaatggcaaa ggaactgaaa atgaaagtaa tatcagaagg
     2761 tgtagagact atttcgcaag tagaatttct aaagcagatt gggtgtgata tggtacaagg
     2821 atatttgttt tctaagccaa tgcctgtaaa agagtttgaa aaaatcgctt ttaaaaaaga
     2881 ataaagcaat gtgtaaataa tgacatatat aataaaggtg ctttaatatt agttgagtat
     2941 actattttag agcatcttta tttttgtatt aatttttacg attttattaa taaaataaat
     3001 caaatgagaa aaaaaatcaa aagaatacaa taatcacttg caaaaaatat actattgtga
     3061 tagacttaaa tcaaaattag gaattgtaaa aagaaggaaa aaataggata ttattttaat
     3121 agattaagat ttaaggtatt gtaatttata tataagataa cgattgcttg taaatttaaa
     3181 ttactttaag aggagggata ttatatgatg ccattgatat tatcaaatac aggtgaagaa
     3241 gtagttataa agaaaatttc gggaagtgat aagacgagga cttttttaaa tagtataggt
     3301 tttgtagtgg gtacaaatgt taaaatagtt tctaagatag atggtaactt aataatagac
     3361 gtaaaagata ctagagttgc attagataaa aaaatggcaa gtagaataat aatataggga
     3421 ggtaatgaca tgaacagact gaaggatatt aagtgtggag agactgtaaa agtcaaaaag
     3481 ttagaaggag aaggagctac acgaagacgt ataatggata tggggattac aagaggtgtt
     3541 aatatattta taagaaaagt agctcctctt ggtgacccaa ttgaggtaac agtcagagat
     3601 tatgaactat caattcgtaa gtcagatgca gaaaaaatta ttgtggaata ataaagatta
     3661 attagagtag ggttaatgta atcatataat gagaatgatt ttctgtatca caaacagtga
     3721 tgtatttaaa taataatgga gggaatggaa atgtcaataa aaataggtct cataggaaat
     3781 cctaactgtg gtaagacaac aatgttcaat gggcttacag gctcatcaca atatgttggt
     3841 aactggccag gagtaacagt tgaaaaaaaa ggtggaaaat taaaaggaaa taaagatgtg
     3901 gaaatagtag atttaccagg tatttattct ttatctccat atacattaga agaagtagtt
     3961 acacgtaatt ttatgctaga tgataagccg gatgcagtaa taaatattgt agatgcatca
     4021 aatatagaga gaaatttata tcttacaact caagttttag agctaggtat accgaccgtt
     4081 atagctctta atatgatgga tattgtaaat aagaatggtg ataagataaa tattaaagaa
     4141 ctatctgaag ttataggttg cccagtggta gaagtaacag cagttaaagg tcaaggaatt
     4201 atggaagctg ctgaaaaggc agttgaactt gcaagtagta acaataaatt aaatttcaaa
     4261 ttaccctttg ttgatgaaag taaggatgca atagaaaaaa ttgaaaaaat aatagaagaa
     4321 aagactccat atatagatgt agaaacacgc tggttagcta taaagctatt tgagagagat
     4381 gaaaatgtta tacaaaaact tgatatttct aaaacaatat tgaatagtat tgaagaaata
     4441 actagaaatt gtgaagatga attggatgat gatagtgaaa gtataataac agccaatcgt
     4501 tatgaattta ttagttcaat aatttcaagt ataataaaga agaatcgaaa aggtaaggaa
     4561 actgtatcag ataagataga taagattgta acaaatcgta tactagcatt acctattttt
     4621 gcacttataa tgtggggagt ttactatata gcagtaagtt cattgggtac tatagcaact
     4681 gattggacaa atgatgtact gtttggagaa attattcaag gcaatgtaag taattttcta
     4741 gcttctttaa atgtagcaga gtggttacaa ggacttgtag ttgatggtct aattggtgga
     4801 gttggagcag tactaggatt tgtaccacaa atcatgcttt tattcttatt attatcaata
     4861 ttagaagatt gtgggtacat gtcacgtgta gcttttataa tggatagaat tttccgtaag
     4921 tttggtcttt caggaaaatc atttatacca atgcttataa gttcaggttg tggagttcca
     4981 ggagttatgt caactcgtac tatagagaat gatagagata gaaaaatgac tattatgtta
     5041 actacattca ttccatgtgg agctaagatt ccaataatag cattatttgc aggggcatta
     5101 tttggaggtg cttcatgggt agctccatca atgtatttct taggtattgc aatgataatc
     5161 atttgtggaa ttatattaaa gaaaactagt ttatttgcag gagaaccatc tccatttgtt
     5221 atggaattac ctcaatatca tattccaagt gcaaaaggtg ttttaataca tatgtgggat
     5281 agaggaaaag catttataat caaagcagga acaattatat ttgttgcttg tggagtaatc
     5341 tggttcttac aatcatttaa ttggtcatta caaatggtag atgctggaga tagtatatta
     5401 gctagcttag gaaatatagt tgcaccaata tttgctcctc ttggatttgg aaattggcaa
     5461 tcatcagtag caacagttac aggtctagta gctaaggaaa atgtagttgg aacatttgga
     5521 gttttatttg gtatatcaga tgcaacagag caagacccaa cacttcttgc ttcagttgca
     5581 agtatgttta cagtagctag tgcgtttgca tttatggcat ttaatatgct ttgtgcacct
     5641 tgttttgcag ctattggagc cataaaaaga gaaatgggtt catggaaatg gacatggatt
     5701 acattaggat tccaaacttt aacagcgtat ataattgctc ttcttataaa tcaagttgga
     5761 agtttagttt taggtactgg aggaagtatt gctggggcaa taatatctat atttatagca
     5821 gtagcagtag tctttgtagt tttaacttat tcaaataaaa atatgaaaaa agaaaaaatg
     5881 ggtaaattaa gttatatgaa aaattaattc atataaataa ggagtgatga atatggctac
     5941 atttattata gcagctattg tagtagtatt aatggcacta gctgtaatgt atatggttaa
     6001 aaattcaaaa aagaatggta gcagttgtgg atgtggttgt tctggatgct caagtagtaa
     6061 gagttgtcat agcgctaaga gataaatagg ataagagcct gataaaaagt taaattgctt
     6121 tattatcagg ttcttatttt ttgtataaat aacaaatttc atatttgaag taggttagac
     6181 tatgctgtaa ttttactgta taattatata taaatatatg attaggaggg attattgcta
     6241 aattaagcaa tataagtgaa tattatgttt aaatttgatg ttacaaacga gcttatgtgt
     6301 atagcaaggg gaagtggtaa attttttgca aaaaaaggtg ctatggtagc ctttaaagga
     6361 aattttaatt ttgaaaaatt gctgttaggt ccaagtaatg gcggtggatt gggaagagca
     6421 ttattaggtc atgttcagag gtcattgaca ggagaacaaa tgccaataat ggtggttgaa
     6481 ggtgaaggtg aaatttacct agctcaaaat gcttaccatg tagatgtaat atcaattgac
     6541 ccaggtgatt ccatttatgt tgagagtgaa aacttattgg cattttctga acaattaaat
     6601 tacagtgtaa aattgattgg ttcaggggta atatctcaaa aaggtctttt tactacacat
     6661 ttagtaaata aaacaggtca aaatcaagat gttgctataa taacagatgg taatcctcta
     6721 attcttgaag gaccttgttg tgttgaccca gatgcattag tagcttggac tggaagagaa
     6781 ccatatccta aagtatcaaa actatcatgg aagactttta ttggtcagac ttcaggagaa
     6841 tcttatcata tggaatttgt tgaacctgga caaatagtca ttatacaacc atctgaaaga
     6901 ttgtctggct taaaaacaga ttctacacct tctgatagaa tgagttcagg tggattaaga
     6961 cttggcatag attaaatctt atgatatagc ttaattatta tttaagatat taaaaaataa
     7021 aaagggtagc agggatagat atgttaaaag gagttttata tgtttgagaa aattcataaa
     7081 aggcttaaag gatattatat ttttttaata attttaattt tatggaagat agtatgtgtt
     7141 ttgggtatat ggagtagcta tattctgcct cctcctgaaa tagtcttaga tacttttata
     7201 aaaatgatat atgacggttc tattttctta aatgtttatg ccagtgtaag aaggatatta
     7261 ataggttttt taataagttc attgatggct ataccacttg gagttttatt tggagcaaat
     7321 aaaaaaatgt atgaatattt caaaccacta tttgaattta tgcgaagtac acctccacta
     7381 gctttggtgc caatgcttat tttatggttt ggtataggtg aagaatctaa aattataatt
     7441 attattttgg catccttttt tccaatattt ttaaatacat tgaaaggtat aaaaaattgt
     7501 gacaataaat taatagaagt aggtaaatct tttaatttat ctaaaaagag aattttttat
     7561 aaaataataa tccctaattc tatgctggat attgttgttg gacttagttt agggcttggg
     7621 tatagtttta gggctataat tggagcagag cttattgcag cttcttcagg actaggatat
     7681 ttgatttctg atggaaaaga tatgtctaga actgatgttg tattggtagg aatttttgtt
     7741 attggatttt taggtatact ttcagattat ctattttcaa agataataac aaaatttagc
     7801 aaagggaagc aggttgattt aaatggttag aagtggattt ttgctagaga atatatataa
     7861 gaaatattta gttgataata aagagcattt agttcttgat aatatatctt taaatatatc
     7921 tagtgaagag ataactgtta tattaggtga aagtggatgt gggaaaacaa cgctacttag
     7981 aattttagca gggcttgaaa atgctactag tggaaacgta tattttttta ataatgataa
     8041 aaagtgtact ccaaaagtag gaatggtatt tcaagaaagt agacttatgc catggctgaa
     8101 tgtaagtgaa aatatacttt tacatactga aaaagataat agaaataaag ttgatttaga
     8161 caagtattta aaaatgatga agcttgaaaa gtttaaaaac tcatatccaa atgaattatc
     8221 aggaggaatg gctcacagag taagtatagc aagagcactt tccttcaatc ccgatatttt
     8281 gctgatggac gaaccatttg cagcactaga ttacttcaca agaagaaaaa tgcaaaaaga
     8341 agttgtaaat atccataaaa atactaaaaa aggtgtagta tttgtaactc acaatattga
     8401 agaagctatg gaaatagcaa agaaaataat tgtttttagt aaaaataaaa ggattaaaca
     8461 atttagtgtc gaagatgaat ataatagaga tttaactaaa aactattata taaacttgaa
     8521 aaaagaaata ttaagggaat tgggggagtt ttaattgatt aaaagaaaca aaaagatttt
     8581 agcagtaatg acatctttaa taatggtgtt ggttgggact gtaggatgtt caacaacaaa
     8641 tgataagaaa gatgaacctg caaaaaaaga ggctagttta cctaaggaaa taaatttaac
     8701 ttatgtaaaa tcaccactta atgttcctag tatattagaa aaaaaggatg atttatttgg
     8761 aaaggaattt aaaaaggaca atataaaagt caatttccat gaattgacaa caggaccaga
     8821 gcaaactcaa gcattagcag caggtgagct agacttttta catgcattag gaggaacgtc
     8881 tgcaataata gctgcatcaa atggtgttgg tcttaaaatt acgaatattt atagccgttc
     8941 accaaaagga tatatgctta taactaataa tgataatatt aaatctataa aagacttaaa
     9001 aggtaaaaaa attgtaggtc caaaagggac tatacttcat caactgttga ttgctgcact
     9061 tgctaaagaa ggctatacta tagatgatgt tgaatatgta aatatggata ttcctagtgc
     9121 tgcttctgca ttagaaagta aaagtgctga tgtagcaatg cttgcaggtc cagtggcatt
     9181 gaaaacgata aattcaggtg ccaaaatgat tgtaaatgga gaaggattag taagtggaat
     9241 aatagttact gctgtaagtg atgactttgc tgaaaaatat cctgaattag taaaaagatt
     9301 tatgaaggtt catgaagaaa ctttaaaata tatgaatgaa aacaaggatg aagtgatgga
     9361 tgttgtatct aaagaagttg gtttatcttt agatgaaaca aaagaaatgt attcttggta
     9421 tgactttagt tctaaaataa cggacaagga tataaaagaa ttagaagata cacaggaatt
     9481 tttgatgtct aatggaatgc aacaaaagaa aataaatatt aaagatatgt tgtacaatca
     9541 aaattagaat actttaaaat attaaagtgg aaaatacacc cttatttatt atggaaactg
     9601 tatggtaaaa gagggtgtat ttttattatt atacaaaaac acaattaaaa acagcttact
     9661 attttatcta cacttatgta gtctagtaat tttctcattt tcttacggtg aagtaaaaac
     9721 ttcgaatatg tgtcctcgga ttgttttgtg tcaccataaa ctttccaata tttaatcata
     9781 gaaacttcat aattactata tcccataaat ccaatcacat ctttaagagg atatcctaaa
     9841 aaaataccta tttcatcagg aaaaacatca ctcttaagtt tatctactaa atgctctaaa
     9901 taagcattaa cattcgtatt ttgtttgtat cctaaaaact ttaaaaagtt tactgttttt
     9961 ttacatttta atttttctga taatgctttt ttatttacaa ataagatttt taatcccttt
    10021 ttttctttat caataactat aaaatctatt ttcttacact tatatagata tttataaata
    10081 tcattaattt tttcctctct atttacatca gtaaacgaaa tatttattat ttcagcaggt
    10141 ttagagccta aaaagactgg acc
//
LOCUS       DAGSHT010000120         9873 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGSHT010000120 DAGSHT010000000
VERSION     DAGSHT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.124"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1343
                     /locus_tag="MJB72_002562"
     CDS             <1..1343
                     /locus_tag="MJB72_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBY8263646.1"
                     /translation="SIICILINILISILIVGFNSRSSKYMLSLNGKKSYGFVNIMHFT
                     LKIVFISFLILSINNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVI
                     KNAKIKGFYKNLVEEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINK
                     NYLKVNPIYSNGKEIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVEN
                     MYNKELNRELNKTKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNI
                     DDSFYLSYISRCVYFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEK
                     YLNSEIFTIIIIAISNLMITYNIVASYYERNKYKLYIKKIFGYSATARSMLLIVSLIL
                     TNIIPIGIISTRVDLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            1348..1965
                     /locus_tag="MJB72_002563"
     CDS             1348..1965
                     /locus_tag="MJB72_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263647.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            2154..2498
                     /locus_tag="MJB72_002564"
     CDS             2154..2498
                     /locus_tag="MJB72_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBY8263648.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            3068..3934
                     /locus_tag="MJB72_002565"
     CDS             3068..3934
                     /locus_tag="MJB72_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY8263649.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            4047..4451
                     /locus_tag="MJB72_002566"
     CDS             4047..4451
                     /locus_tag="MJB72_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBY8263650.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            4615..5064
                     /locus_tag="MJB72_002567"
     CDS             4615..5064
                     /locus_tag="MJB72_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8263651.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(5220..5597)
                     /locus_tag="MJB72_002568"
     CDS             complement(5220..5597)
                     /locus_tag="MJB72_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY8263652.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            5859..6776
                     /locus_tag="MJB72_002569"
     CDS             5859..6776
                     /locus_tag="MJB72_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBY8263653.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            7260..8678
                     /locus_tag="MJB72_002570"
     CDS             7260..8678
                     /locus_tag="MJB72_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBY8263654.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            8754..9566
                     /locus_tag="MJB72_002571"
     CDS             8754..9566
                     /locus_tag="MJB72_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8263655.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTNILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         3930 a         1006 c         1589 g         3348 t
ORIGIN      
        1 tttcgattat atgtattttg ataaatattt tgatatctat attaatagtt ggttttaata
       61 gtagaagttc taaatatatg ctatctctaa atggaaaaaa atcttatggt tttgtaaata
      121 taatgcattt cacattgaaa attgtattta tatcatttct tatattatcc ataaataact
      181 gtattttaaa ttataatatg ctacaaactc agttaggaaa tctatctgag tgggataaag
      241 caaaaaatgt ttataatctt actcttaaag atacaggtga acaatcttta ggaaaggaag
      301 aagtaattaa aaatgccaag attaaaggtt tctataaaaa tttggtagaa gaaaaagatg
      361 ctttcttaat agattcaact aattttgaaa aacttgaaga tggaagttac atgtatgaaa
      421 taaattcaaa gggtaaaaac ccagaagtaa gtcaatatgg taaaaatata aaaataaata
      481 aaaactatct taaagttaac cctatttact caaatggcaa agaaatattt aatctaatcg
      541 attatgacga taagactata aatttattag ttcctaaaaa gttacaagta catgaaaatg
      601 agattaaaaa agagtttaga gaattatttt attttgaaaa aatagaagta gaaaatatgt
      661 acaataaaga gttaaataga gagttaaata aaacaaagaa gtcagattta aatgttaata
      721 taatatatgt agataataat caaagctatt ttacttataa ctcccttgtt atggatgaca
      781 ataggaatct cattgatgac ccaatagcaa tagttgacat agataatatt gatgattctt
      841 tttatctttc ttatatatct agatgtgtat attttaattc taagaaatta gatgcccttg
      901 cagatataag taacattatt gaagctcaag gtgtagaagc gcatatccaa agtttacatg
      961 ctgtttataa tgagtatggt ttagaaataa ataaactaga aaaatatttg aatagtgaaa
     1021 tttttactat aataataata gcaatatcaa acttaatgat tacctacaat atagtagcta
     1081 gctactatga aagaaataaa tataagttat atattaaaaa aatatttggt tattcagcta
     1141 cagctagaag tatgttgtta atagtctcat taatattaac aaacattatt ccaataggca
     1201 ttatatcaac aagagtagat ttaagtaata atattatctt atttggactt ctaatattag
     1261 ttatagaagt aattgtatct attgtattag acaagattat ctcaaatagt tcatttaata
     1321 aaattataaa aggagaacat taaaaagatg atttcattaa taaatatatc taaaaaattt
     1381 aaagataaat ctataataga caaatttaac ctagaaattt ctaagggcga gtttgtagcg
     1441 ataacaggaa atagtggttc aggtaagaca actttactta atataatggg attattagaa
     1501 aaacctaatt ctggtgatat tataataaat aatataaaaa atccaaatag taaacagata
     1561 agaattttac aaagggattt ttatggctat ctatttcaaa attatgcttt aattgagaat
     1621 gaaaatgttg aaaataactt gaaaattgct ttaaagtatc aaaaaaatat caaccataat
     1681 gaaaaaataa attacgcact tgagtctgta ggtcttagaa aatacaataa aaaaaagata
     1741 tacgaattga gtggaggaga acaacaaagg gtagcattag caagagtaat attaaaagaa
     1801 tgtgaagtaa tatttgcaga tgaacctaca ggtaatttag ataaaaaaaa tagagacata
     1861 gtatttgata tacttaaaag tctaaataag aatggtaaaa ctatcgtatt tgtaactcat
     1921 gatttagagt tatcggagca ggcagataga gttataaaat tgtaataata tcaactataa
     1981 taaatataag aaagagatta cagctaaatc aagtttaata ataatacgaa tagaatttaa
     2041 acattaagta aattagtagt ttgtaaatat aagtataata tgatatcata aaggctcaaa
     2101 agaaatttta tataataaga aatctaaaaa taacaaatgg aggacatata aagatggaaa
     2161 atttaccaaa ttgcccgaaa tgtaattcag aatatactta tgaagatgga actcttttag
     2221 tttgtccaga atgctctcat gagtgggcat taggattggg agctgaagaa gattcaaata
     2281 ttatcaaaga tgtaaatgga aatgtattaa atgatggaga ttctgttaca gtaattagag
     2341 accttaaagt aaaaggaagt tcatcatcta taaagattgg aacaaaagtg aaaaatatcc
     2401 gtttaatcca tgactcatca gatggacatg atatagaatg taagatagat ggttttggag
     2461 ctatgaagct aaagtcttct gttgttaaaa aagcgtaata ttataaataa gctgtagtgt
     2521 ttattgtttt tcagtacagt ataaaaccat aagaatctat taagtaataa aagtttgttt
     2581 atttatcttt atattttctt atggttttat taaatataaa ttttattgaa caccaaaata
     2641 ttctctcctc tacaaataag ccaatcttat ttaaactaat atactttgta ctatttatac
     2701 tgaaattgta aatttaaatg ctatgttatt taggtttgta tagttaattt atatatttct
     2761 gattttataa attgtattta ttattatatc ttgattctaa aatcattaaa aatttcaact
     2821 tctataaaga tttacaattt atcaaataca ttatgagtat ataaacaatt tattattaca
     2881 gtgctacatt taaaataagt cttagtataa aggtattgtt acaagtataa agaaacttat
     2941 gtatgctatt ggaataaata ataccttaaa ttataattaa gaataagaac tgttaaataa
     3001 gggacttgat tatcatttag ttatagataa tataatatta aaaataaagt ttaagaggag
     3061 aaaaaatatg atagatatac ataaaaatat atatgataat aagttatttg aggagctaaa
     3121 gattgactgt aagaagtgct ttggactttg ctgtgtagct ctttattttt ctgcttcaga
     3181 tggatttcct atcgataagg aatcaggaaa accttgtatt aatttacaac cagattttaa
     3241 atgttctgta cataatagtc ttatgaagcg aggttttaaa gggtgtactg catatgattg
     3301 ttttggttca ggtcaaaaag tagcacaagt aacatataag gggatagact ggatgcaatc
     3361 accagaacta acaaatcaaa tgtctgaagt gtttttaatt atgagacaat tacatgaaat
     3421 gttatggtat cttaaagaag catctgtatt aaatatatca gatacaatca aatcaaaaat
     3481 tgatttaata attgaagaaa ctgaaaagat aactaatatg ggcccagaac agataattaa
     3541 tttggatatc atatcacata gaacaaaggt taatttatta ttatcacaag cgagtgagtc
     3601 tgtgatggga aaggttaaat catttatcaa gacttctact cttaaaaaca tgaaaaaatt
     3661 aagtaaaaat atagatttaa taggagcaga ccttagaaaa ataaatctaa taggagcaga
     3721 tttaagaggt agatttctaa tagcagcaaa tttaagaaat acagatttga gtggggcaaa
     3781 tttaatagga gcagacttaa gagattgtga tattagaggt gctaatcttg aaaatagtat
     3841 atttttaaca caattacaag taaatactgc aaaaggagat tctagtacga agttaccagc
     3901 ttcactaata cgccctaaat actgggaaaa ataattaaat taatacataa ttttacaaaa
     3961 tatgaacttt tttgacttta attcatatta tacattagag attctttaca agaatttctt
     4021 aatataatat agggagagat ataaaaatga gttatcaaaa ttataaatgt gaaagagatt
     4081 atgtgtatga tgattataaa tacaataagt gcaacaagtg ctataatgat tatgaggaaa
     4141 tgacacatgt tcatgaatat tcagaaagtg ttaagttagc agaagaatgt gaggatagac
     4201 ataaccaccg tgcagcaggg gttactggtg aagctatacc aataaatgga ggtacaaatc
     4261 atgttcataa aataaatgat aatgtagatt tccttgacca tttccataag atatgtgtta
     4321 caacagggcc agcaattaga atacctggaa ctgataaaca cattcactta atatgtggag
     4381 aaactactgt taatgatggt cattgtcata aattcctttt tactactcaa atagaagcac
     4441 ctttagtata agctgatgtg aattattgta gtgaatttgc tagattatag tataatatat
     4501 aatagataat atttaaactt aatgaggact atgatattta accttcgtag tcctttctaa
     4561 gttttgtata aaaaagtgtt atgcaattaa aagtgatata gggaggaaat ttttatgaaa
     4621 tctggaataa ttcttgaaat gaaacaaata aattcaacag aatatgacga aatgttgcaa
     4681 gtctgggaat catcagtaag agcaacgcat gattttttaa cagaaaaaga tattgaatct
     4741 ttgataccat tagttgagat tggacttaaa gaagtagaaa atatagtttg tataaaagat
     4801 aatgatataa taaagggctt tataggaata gataaagata aaattgagat gctctttata
     4861 gaagataaat atagaggaaa tggtattggt aaaaaattaa taaaatatgc tatagataaa
     4921 tacaatgtaa aatatgttga tgtaaatgag cagaataaaa aagctgtagg attttatatt
     4981 cacttaggat ttaaagtgtt tgataggtct gaaatagatg gacaaggtaa cccatttcca
     5041 atactacata tgaaattaat ttaaagttat acttctcact tattcgcaaa aatatttact
     5101 taaattttta tacaaataaa agtcgtgaca gtcttaggac acgacttttt tactacacta
     5161 ttaaattgaa atttatctgt tctatattat gattattaga tgtagttttt ttatttttac
     5221 tatgtactta aataccaagt atacccatac ctatcaagta cttcagcagc gccatcattt
     5281 tgatgtggtg gtgggtgtgg taaaaattct tgtacaagtg taccaccttc ggcaagtttt
     5341 acagatattt cttctatcgc ttctcgagat ggtagaacaa cacaaatcgc catgccagaa
     5401 attacaacag gcttgtctgt atccgcaccc attatacaac cttcgtattt tgtgaaagta
     5461 acacttgcgt gcataatagg attgtttgga ttgctgtcta atcgcgcaaa tatagttttt
     5521 cctcctccga aagcctcagc ataaaaatta aatgcttttt cacaagtacc attaaatatt
     5581 aaatatggag ttagcatgac caatatatcc tccttgatat agaataattt atagttgcac
     5641 ctgataatct aaattatgat ttagtaaatt gtatcatagg gaaaccttga agtatataat
     5701 tttttattat tatattatac aaatttatta atttaaaaaa atgttatatg tacggaaggt
     5761 ttacaggata aaatataata aaaagattaa ggtgacatta taaaaaatgt atattgacat
     5821 tgacgctacg taattgtata tactttgtct taaaggagat ggtaaatatg aatattatag
     5881 aaattcgctc agataaaata tacaagaaga taatggatgc accaataaac aaaaaagaag
     5941 atatatacag atatgaattg atgaagcctt ttgaatttaa gtggaagtgt atgaatgttc
     6001 caatagttgc tagacagaaa ggtggatatg atgtaattat agcaagtgaa atgttagggg
     6061 ttttatcgcc taaggatatt gatgaaaagc aaaaaaagaa tataaatgtg ttatctgctg
     6121 ataaaatttg ggccacttgt aaagaaacca tagaaaactc tataaatgct tttataaaag
     6181 aagggtatga tttaaacatt aaggactata aatattcaat attattggcg aatccaaata
     6241 gtccttatac aatattaagt gatggatact ggggtgatgg tgggattcct ggatatatat
     6301 ttctatcatt ggttcctaat gaatatacta tcaatagatt accagtatta atagcacatg
     6361 aatgtaatca caatattaga tttcagttta tagagtggaa taataatata acattagaag
     6421 aaatgatgat aaatgaaggt cttgcagaaa attttgcaac atggatgttt ggagaggaaa
     6481 tgttaggacc ttgggtcagt agaacagata tcgaaacatt aaatacttat ataaagccaa
     6541 taataaaaag tgctttaaaa gaaactggat ttcaaaatat aacatcttat ctttatggtg
     6601 atgatatagc taaaatgcaa ggatattttc cagtagggtt gccttattgt gcaggatatg
     6661 cttgtggata ttatatgatt aagtattatt tagaaaagac aaataaatca ataatcgaag
     6721 cgactttatt gccttatagt gagataatcg aagcagtaaa agagttttgg gaataaattt
     6781 tataaattgc ataatttgaa ataaattatg caattatttt atttttaaga aaaaataaat
     6841 tgacaataat ttataagaaa tatacaataa tatatataaa gaaatatttc aatatttaaa
     6901 agttttaaga tttcatatct agagtaccta acattacatg ttagtttctt gttacttatc
     6961 taattacttt atatttcgta gttatatata aaattactat aaaaaatgaa atatattaaa
     7021 caatcaacta aaaaatgaaa tattaataaa aaaaaattca aattataaat attaaatgaa
     7081 tataattaaa acaaatatat aaatttaagt ataacactca cattttaatg tattgtgaaa
     7141 tttttgttga actttatgta ccataactta taaaatttat gtcatgttat atttgaataa
     7201 gtaataaaat tgtagatgta aattaatcta caaaatttat atgaaatgga gttgatgcta
     7261 tgacagaatt aatgattata agtgggttag tactattgat ttgtataact tctagcaagg
     7321 tattgtataa atttggagta ccaatattat tgatttttat cttactaggg atgttatttg
     7381 ggtctgatgg gatggtagga atctattttg ataactatga actgacaaaa caattatgtt
     7441 ctattggact aatatttata atgttttttg gagggtttgg aacaaactgg aatatggcta
     7501 aacctgttgc tataccttct atattaatgt cttcactggg tgtaataatt acggctggcg
     7561 ttacaggtat gttttgccac atggtaatag gaactagttt acttgaaggt ttattaattg
     7621 gggcaattgc agcttcaaca gatgctgcat ctgtgtttgc tgtgttgcgt tctcaaaaac
     7681 taaacttaaa gggttcactt gcatcattat tagaagttga aagtggaagt aatgacccaa
     7741 tagcgtatat gttgacacta attcttttga cattgatgaa taattctggg gtagaactta
     7801 ttgcaccaat ggtaataaaa caaatagctt ttgggttggc tataggattt atactagcaa
     7861 aattatctgt ttatatttta agacattcaa acttcgagat agaaggattt tatactatat
     7921 ttataacagc tattgcaata ctttcttatg catttagcga gtatcttgga ggaaatggat
     7981 atttaagtgt ttacatttca ggtattatta taggaaatag taggatacct cttaaaaaaa
     8041 gtttggttca tttctttgat ggaatttcat ggattatgca aataatgtta ttctttatgt
     8101 taggattgtt atctttccct tctaagttac caaatgtatt tggtatagca atggccatat
     8161 ctatattcat gatagttatt gcaagaccat tagcaacatt tatagttcta gcaaaattta
     8221 aattctcaaa taaagaaaaa ttattcatat cgtgggtagg tcttagaggt gcggcttcag
     8281 tagtttttgc aatttatgct gttactcaag gagtagccat agagaatgac atattccata
     8341 taatattttt tatagcgttg ctttcagtag gaatacaagg ttcattgata ccaactgtag
     8401 ccagaaagtt ggatttagtt gatgataata cacctgtatt aaaaacattt aatgattatg
     8461 atggagaaat tagctctaaa cttatcgaag taaatgttga tgagggtaat aaatgggcta
     8521 ataagagtat aatagattct aatataccag acgatatatt aatagttatg ataaaaagaa
     8581 aggaacaggt tttaatacct aaaggttcaa ccattataaa aacaggggat attttggtat
     8641 tgagtggaga tagaattgaa gaattaatac aattataatt tattttgtat tgactttaac
     8701 ctaaggggaa agtttaaact taaattaatc aaatctaatg cgcaggtgat gagatgtttt
     8761 ctataggaat gttttctaaa ataaataaaa taactacaaa gacattaagg tattatgaag
     8821 atatagggtt gttaaaacca gaatatgtag atgaatttac aaaatatagg tattatacaa
     8881 cagagcaatt acctaaatta catcagataa taactttaaa acaaatgggt ttatcattag
     8941 ctgagataaa aaaagctgta gaaaatcctg aagaagttga aaatatttta atgaataaag
     9001 aaaaagaaat gttggatgta attaaaagtg aagaatataa aataattaaa ctacgtaatt
     9061 accttatgaa tttgaaagga gaagtcaata tgaatgatgt aattattaag tctttaccaa
     9121 aagttaaagt tgcttctatg agaaaagtgt taaaggggta tgatgaactt tttaatctat
     9181 ttccaaatgt aatggctgaa gaaatgatga aaacagggtg tacatgtgca gttcctgatt
     9241 attgttttaa tatatatcat gatggtgaat atagagagac tgatatggat gttgaaatgt
     9301 gtcaagctat aaatgattta aaggaagata cgaatatatt aaaatttaaa gaattagatg
     9361 cagttgaaaa tgcagtttgt atattacaca atggtccata ttcaacatta ggaaaaactt
     9421 acgcagatgc atttaaatgg atagaggaca ataaatataa agtggttgga aatcctagag
     9481 aatcttatat agatggtata tggaataaag agagtgaaga agactggttg acagaagttc
     9541 agataccagt gaaacgcatg ttataatatg tctacaaagt ataaataaga ataaaaaagg
     9601 tggtgtctca aagtaaacat tttagtttat gagacactct ttttcatatg aaatcaagta
     9661 tatttttata attacgatta tgaaaaaagg cttatctata tttttagaca gcctcttatt
     9721 tttttattat aatatttttc taaagatgct ctatttataa atttttatta aagactttat
     9781 aaataattgt atttaatata caaaatatat ataaatattg atgttttata tataaagttg
     9841 tatatttttt taaggatata aagtaaaaaa ata
//
LOCUS       DAGSHT010000121         9763 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.125, whole genome shotgun sequence.
ACCESSION   DAGSHT010000121 DAGSHT010000000
VERSION     DAGSHT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.125"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1206
                     /locus_tag="MJB72_002572"
     CDS             <1..1206
                     /locus_tag="MJB72_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263656.1"
                     /translation="DVVLTDFQSNTTVENIFNYIESKYTIIISKADRVYLYNHFITKS
                     SLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKSILNSKSYNL
                     NKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIGAGIERFFQS
                     NIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASELSDVDIIIST
                     IPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLFIVNPKIKDK
                     DELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELSSIRTKICIS
                     ILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDSISNCNDFDN
                     FLSIIKSIV"
     gene            complement(1466..2227)
                     /locus_tag="MJB72_002573"
     CDS             complement(1466..2227)
                     /locus_tag="MJB72_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8263657.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            2446..2922
                     /locus_tag="MJB72_002574"
     CDS             2446..2922
                     /locus_tag="MJB72_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY8263658.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            3020..3172
                     /locus_tag="MJB72_002575"
     CDS             3020..3172
                     /locus_tag="MJB72_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBY8263659.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(3332..3520)
                     /locus_tag="MJB72_002576"
     CDS             complement(3332..3520)
                     /locus_tag="MJB72_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBY8263660.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            3881..4231
                     /locus_tag="MJB72_002577"
     CDS             3881..4231
                     /locus_tag="MJB72_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBY8263661.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            4250..5893
                     /locus_tag="MJB72_002578"
     CDS             4250..5893
                     /locus_tag="MJB72_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBY8263662.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            5974..8031
                     /gene="recG"
                     /locus_tag="MJB72_002579"
     CDS             5974..8031
                     /gene="recG"
                     /locus_tag="MJB72_002579"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBY8263663.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            8090..8644
                     /gene="rsmD"
                     /locus_tag="MJB72_002580"
     CDS             8090..8644
                     /gene="rsmD"
                     /locus_tag="MJB72_002580"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBY8263664.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            8646..9143
                     /gene="coaD"
                     /locus_tag="MJB72_002581"
     CDS             8646..9143
                     /gene="coaD"
                     /locus_tag="MJB72_002581"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBY8263665.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            9147..9671
                     /locus_tag="MJB72_002582"
     CDS             9147..9671
                     /locus_tag="MJB72_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263666.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
BASE COUNT         3850 a         1072 c         1658 g         3183 t
ORIGIN      
        1 gatgttgttt taacagattt tcaatcgaat acaactgttg aaaatatatt taattatatt
       61 gagagtaaat atactataat tatttcaaag gcagacagag tttatctata taatcatttt
      121 ataactaaaa gttctctttt ggataacgtt tccaataggg tagatactaa aattatagag
      181 tatgttgaag aaattttaga agtaatcaac aatcaatata cttttgacct tagaaatgat
      241 agtgtattat ttgatgattt agtactgcac tttaaatcaa ttctcaattc taaatcttat
      301 aatttaaata aggtgaatcc ccttataaat acaataaaga gtaattatcc tttagcattt
      361 gaaataactt taaatgctat agaaaaagtt tttaaaaata gtatttactc tttaacagaa
      421 gatgaaattg gatatgtatc cctgcatata ggtgctggaa tagagagatt ttttcaaagt
      481 aatattaagt gtaaaaacgt agtattagta tgtggttcag ggtatggttc aagtaggtta
      541 ttagaggttc aattaaacaa agtttttcat gataaaataa atatacttca atgtctatca
      601 tttaatcaat ttttagctag tgaattgtca gatgtagata taataatatc gacaattcct
      661 ttaaatcatg atagtattcc tattgtatta gtggatttaa aactattaaa aaaagatata
      721 gagaatatat ctaagtcaat cactaataat tcccatattt atagtaatct tttggacaat
      781 ttctttgata aaaatttatt tattgttaat cctaaaatca aagataaaga tgagcttata
      841 aagttgatgt gcaacaaatt aactcaaagt gaaattgtat ttccatcatt tgctgaatct
      901 gtattttaca gagaaagttt atcttctacc aatatagatg attttttagc tatacctcat
      961 cctatggaat taagctctat aagaactaag atttgtattt ccatattgaa tgaaccaata
     1021 tattggagtg aaaattctac tgttaaatta atcatgatgt tggctataaa taaagatgat
     1081 tatataaaaa taaactccat ttatgatatc cttttaaaga taattcatga caatgatatt
     1141 agagatagta tatctaattg taatgatttt gataattttc tttccataat caagtctata
     1201 gtatagattt ttatattaca taagttttaa aaactttaat aaaatatttt ttgtttttag
     1261 cataaatagt ataagtaagt tttgtataat ttgttattat tttgtattga ttataattat
     1321 tatacaataa agattctgtc tcaaaataga gagagaaacc tgtttttcat tgataaaatg
     1381 ataaaaatat atctgatttc atacaaaaaa gagtgtctca tgaactaaaa agttcatttt
     1441 gagacaccac ctttattatt ttatattatt tatctcttgt atatattact atagcatccc
     1501 ttaaaaactt agctaatcca ggttgttctc tatcataata tgatgtaaaa cgttcatcat
     1561 atacatacat ctgtgctaga gaggaatgag cttctttaga gtatgtgttc caagtgtaac
     1621 acaaccattt atgatgtaat tttgcaactt cctgagccag ttcactagag gcatcaccag
     1681 ttttaaaagc ttttttaagt ttactaatta tttcgatatt taggttttgc caattttcat
     1741 aatcttgctt agacatattt ttaagttttt tatttgatgt atctataata tctttaccat
     1801 atttctctct aatttcagtt ccataatttt tttcattttc atcaatcatc ttttctttaa
     1861 aaccttcaaa tttttctata tctgtcatag tgtattttcc ctccttcaga gctatagttt
     1921 tagttacatt ttctattagt aaatcaattt gctttctttt ggctagtaat ttattatggt
     1981 gttcttttaa cgcacttaaa ctatcgaagt ttggtgaatt tataatgttt tttatatttt
     2041 ctaagttaat tcctaattct ctataaaaaa gtatttgttg aagtttatta acttcatttt
     2101 gaccatatat acgataacct gaagaattta ttttaagtgg cttaagaaga cctatttcat
     2161 cataatatct aagtgttcta gtacttatac cagctagttt acttaatttt tgaactgtat
     2221 attccataag tagtacctcc tataaattaa atatacactt ttacgcaacg taaatgtcaa
     2281 tacattaaaa tataaaaatt tatatctttt aataaaaata taaaaaaata tctttactgg
     2341 atattttaca cacaactgtc aatactcact agtgaaaatt aaaaaataaa atttatagac
     2401 tatctttact aaaaaataga aggtctatag tctaggagga actgtatgaa tttaaaaaga
     2461 agcaatgaaa aacgagaaga gtacagaaga atgtattcag ataaagaaag ttttttaagt
     2521 ttgattcaaa attttacaag tgtctcaata gcaaaagaat taactcttaa aaatatagag
     2581 ttagaaacat cttttatatg tgaatataaa ggaaaagaag ttgatataat ttataaggta
     2641 ttttctaaaa gcggcaaagt atcacattat atagtgttag aatttcaaac agaaatggat
     2701 actgaaattg tcccaagatt gaaatcgtat agagaacaaa tttggaaaag ttttattatg
     2761 aaaaaaagcc ttgaagaaat agaagataaa aattttaaac ttcctaaagt tataccagtt
     2821 gttttatata gtggacctga aaggttaagt agaaaaaaag gaattttgga tattgtagaa
     2881 tctatatcgg agttcagtaa taattctagt atgaaaaatt aaaattacat aatgtgttta
     2941 ttggtctaaa atggaaaact gtcgcataaa cttgaatatg aaaatattta tagatacagg
     3001 ttctatgtgg aggtgttcca tgagaaataa caagtctaag gtattgctag gaatactttg
     3061 tctagcaaca ggaatggctg taattctgtc aatgttgctt cctggctgga tttggtcagc
     3121 acttaccgca ttaatactta ttggatgtgg agcattatta tttttttgtt aaaatagttt
     3181 taactataaa gtgtggggtg agtattgatg aaagtagtaa caataaaatt accaaaatgg
     3241 atttcaagta taatcctaaa atttatgaga aagagtaaaa atgattaaat taaaaagcct
     3301 acttacggaa gtaggctttt aaacgtggtt actaagctct ttcaatttta ccagaacgta
     3361 aacatctagt acaaactttt actctcttag gagctccatc tataatcgct cttacgcttc
     3421 ttaagttagc tgaccatgtt cttttattat gtttatttga gtgtgatact tggtttccag
     3481 aaaccttacc tttaccacat acgctacata cttttgccat ctacgcacac ctccttaaaa
     3541 tgataactat ttaaagacta aaaacatatt tatattatca taaataacaa aaatttgcaa
     3601 tagaaatata tatttgcctt acacatattg aagaatttct tattatcata taaaattata
     3661 tatataattt tatataaagt aaatatattt ggaaaataat tattattttt ctttgaatat
     3721 atatactata gaagaaattt tatccaaaaa cttttctagg tttaaattat accatattta
     3781 tagtatgata tgattataat tttatataat aaataagtaa aaatagtaaa aaataaatta
     3841 agaaatgttt ttaataaaaa taagcttaag ggggtcaacc atgagtgcaa aggtaacgaa
     3901 tcaatatgga aatatagaaa tagataatca agtaatagct caagtcgtat atagagctgc
     3961 aatggaaagt tatggcttag taggtttagc atataagtct aaaggaatag tagagctatt
     4021 aaaaggcgag aacgcaacta aaggtgtaag agttgaggaa gtagaagaag atgccatagc
     4081 aatcgaactt tatgttataa tacaatatgg tacaaatata tctactgttg ccaataatat
     4141 aatagataga gtaaaatatg tagttgaaaa aatgactggc gtaagagtta ctagaattga
     4201 tattaatgta caaggaataa gagttaagta atttcatagg aggaaacaaa tgattcaata
     4261 tatagatggt aaaaggttaa gggagatgtt tatttcaggt gcaaacaatc tccaaaacaa
     4321 taaagagtta gttgacaagt taaatgtatt ccctgtacca gatggagata caggaactaa
     4381 tatgtcatta acaatatctt atgccttaaa agaattagca aaagttgaaa atgataatat
     4441 atcagatatc ggaaaagcat tatctaaagg ttctttaatg ggagctagag gaaactcagg
     4501 agttatactt tcacaaataa tcagaggtat agctaaatct atagaaggaa aaagtaaatt
     4561 atcaacagaa gatttagcaa aagcttttaa aaatggttca gacacagcat ataaagctgt
     4621 aataaaacca attgaaggaa ctatacttac tgtagttaga gaaagtggag agtttgcaat
     4681 aaaaactgcg aaaaaagaga aagatgtagt aaagttttta agtatgcttg tgaaggaatc
     4741 aaattcttca ttagaaagaa ctccagacct tctaaagaat ctaaaagaag ctggtgtggt
     4801 tgactctggt ggaaagggtc ttgtacttat atatgaagga atgttagctt ctataaaagg
     4861 cgataacata gaaataaaaa atgttgacct agatactaat atttcaacaa gtatggattt
     4921 tgcaaagagt actactagca cagatgatat aaaatattgc tattgtacag aatttatttt
     4981 agaaagttct aaggtagaag atactaaaat aagagacata atgatggctt atggtgatag
     5041 cctagcagta gttggagatg atggagtcat aaaggtacat gtacacacta atgacccagg
     5101 aaatgtactt caagaagctc taaagtacgg tcaattatta actataaaaa tagaaaatat
     5161 gaagttacaa catgaaaata cattactgga tgtagaagaa aagaaagaaa atgatagtga
     5221 acctttagaa gaagaaaaag agtttgggtt tatagctact tctatgggtg aaggtttggc
     5281 aaatatattt aaagatttcg gagttgacca tataatagaa ggtggtcaaa ctatgaatcc
     5341 aagtactgaa gattttatga atgctataaa gcatataaat gctaaaaata tatttatttt
     5401 cccaaataac agcaatataa taatggctgc taatcaggca aaagaattga gtgataaaaa
     5461 tataatagtt atacctacaa aaaatacgcc tcaaggattt gctgcattag taacttttaa
     5521 tggagaatta agcgaagatg aaaataaaga agctatgatg aatgctttaa actcagttaa
     5581 atcaggtcaa gtaacttttg ctgttaggga tacagtgatg aatgaaattg atgttaaaga
     5641 aggtaatata attggaattg cagaaggaaa tttattatca gcaggagatt atgtggatga
     5701 agtaacatca aaccttattg aaaagttggt tgatgaagat actgctataa taacattatt
     5761 ctttggtgaa gatgttacag aatctcaagc aaatgaacta cgtacttctt tagaagaaaa
     5821 gtttgaagat gtagatgtag agttatatta tggaggacaa cctctttatt attacttaat
     5881 ttcagttgaa taatgattat aagaacctgt atgaccaagt agcttttata aaattggttg
     5941 tacaggattt ttgtgtagta ggtgatatat ttgttggatt tatataaaga tgttcaatat
     6001 gttaagggaa taggacctaa aaaagcagat aaattaaata aactgggtat atttacacta
     6061 aaagacctat tgtattattt tccaagacaa tttgaagata gaaataatct aaagaaaata
     6121 gcacaattag aagatggtga gaaagttacg ataaaagcag taatatcaag tataaataca
     6181 tttagcccaa aagaaggtat gactttaaca aagattgatg taaaagatga gacaggctcg
     6241 gctaaattag tgttttttaa taagagttat ataaaaaata cttttcgtcc aggagattca
     6301 atattagttt ttgggaaggt taagaaaaaa tttaataact tagaacttac atcatgtgaa
     6361 ttagaatatc ttactaattc acctaagaat acatgtagat ttatgcctgt gtaccagctt
     6421 acatacggtg ttacaaataa agaaattatg agtataatta gaacagtact tgaagataaa
     6481 gaattaatta tacaagaata tatgccacaa agaattatag aaaaatatag gctatgtagt
     6541 atagattttg cagttagaaa catacattca ccaagtagta aggaatcatt aaaaatagct
     6601 ttatatagaa ttgtatttga agaattactt atactacaac tagggttgtt tgtatttaaa
     6661 agtggcagaa ataaagaaga tggaataaag tttgaaacaa gtaaagactt aaaaaaaata
     6721 ataagtgctt taccatttaa actgactaaa gctcaaaaca gggcattaga tgaaattata
     6781 caagatatga atttagagaa aataatgaat agattggtac aaggtgatgt agggagtggt
     6841 aaaacagtag tagctctctt agcacttgca aattgtgttt taaatggtta tcagggcgct
     6901 ttgatggcac ctacagagat attagcaggg caacattata tttcacttac tgagtctcta
     6961 aaagattttg gaataaatgt aggtctatta ataggtagtc ttacaaaaaa acaaaaggat
     7021 acagtattgg aacaaataaa gaataatgag attgatatat taataggtac acatgcacta
     7081 attgaagata aggttgaatt taataatatt ggtttagtta taacagatga acaacataga
     7141 tttggtgtta tgcaaaggag caaactttca ttgaaaggag caaatcctga tatattagta
     7201 atgacagcaa caccaatacc aagaacttta gcacttatac tctatggaga tttagatatt
     7261 tctattatag atgaacttcc tccaggaaga cagccaatag aaacaattgc tattgaaaaa
     7321 agtaagcgag atagagcata taataatctt gtaagaagag aggttgaatc aggaagacaa
     7381 gtgtacattg tatgtccctt ggtagaagaa agtgaagcca tagaagccaa atcagcagtt
     7441 gaacttgttg aggagttaag agcggaatat tttcacgact taaggcttgg gttgcttcat
     7501 ggtaaaatga agtcaagtga aaaagatgaa gtaatgagac tttttaaaga taaagaaata
     7561 gatattttag tttcaacaac agtaatagaa gttggtgtca atgtgccaaa tgcaacatta
     7621 atgataatag aaaatgcaga aagatttgga cttgcacaat tacatcagct tagagggcga
     7681 gttggaagag gtagtcataa atcgtattgt gtactaattt atgattctaa aacagatgta
     7741 tgtagacaaa gaatggctat aatggaagag accaatgatg gttttaagat ttcagagaag
     7801 gatttagaaa taagaggacc tggggaattt tttgggacaa ggcagcatgg tcttccagaa
     7861 cttaaggtag caaacttatt taagcatata aaaatactga agttagcaca gcaagaagct
     7921 cgatatatat tgggtgaaga taataatcta caattaaaag aaaacatggc actcaaaaaa
     7981 gaaattatag ataaatttaa agatacattg aaagaaattt cattaaatta attttaaatt
     8041 gtgttaaaat attactaatg taataatgtg ctgaagggag attgtcatat tgagagtaat
     8101 ttcaggaaaa gcgagaggat taaaactaaa tactccaaaa aatgaagatg taaggccaac
     8161 tacagataga gtaaaagagt ctctatttaa tatgataaat tcatatataa tggaaagtga
     8221 agttctggat ttatttgctg ggactggttc tttaggaata gagtgcttat ctaggggtgc
     8281 caagtcatgt acttttgtag atattagtaa ggaaagcata gatattgtta agtccaatat
     8341 aaaaaaggca agagttgaaa gtgaaagtat tattttaaac cttgatttta agactgcaat
     8401 agataaactt aaattacaaa atagtaaatt tgacataatt tttatggacc ctccgtatta
     8461 taaaaatatg tttatagaag ctattgaaaa aattgataat tctaatctat tgaacgaaga
     8521 tggaataata gttgtagaac atgatacgaa tgatttattt ccagataaga tatgtaaatt
     8581 ggagaaaact agagacaaga aatatggaaa tactacatta actttttata agatggaggc
     8641 ataatatgga aaataaacct agaaaagcta tatttgcagg aagttttgac ccaattacta
     8701 atggacattt agatattatt tgcagggctt cgaagttatt tgacgaatta caaataggag
     8761 tattaaataa tcctaataaa aagggtctat ttagctttga tgaaagagta aaacttatag
     8821 aaaaaagtac aagccattta aataatataa aagttgtgac ttttgatggt ttattaataa
     8881 attactgtca ggaaaatggg attggagcat tagttagagg tgtgagaagt ggtgctgatg
     8941 ttgattatga acttcagatg gcacatatga atagagagct taatccagat atagagacta
     9001 taattttacc aagttgtaca aaatattcat ttataagttc atctttaata aaagaggttt
     9061 tgctctttga tgctgatata aaaaatttgg ttccaaagat tgttttggaa gaattaaaga
     9121 aaaaaactag tgggggtaat tgatatatga agatagattt agaaatgatt gaactatttg
     9181 agcagttaga ggagatattt agaagtgctt ctacaatccc tttctcacat aaatgcacag
     9241 tggataaaga tgaggtttta gcaataatta atgatataag aactttaata ccagaagaag
     9301 taacacaggc agtatggatt aataaagaaa gaaataaaat aataagtcaa gctaagcaag
     9361 atgctacaaa tatagttgaa caagcgaaaa aagaagctga gagaatacaa caagaatatc
     9421 aagataatat agaggaatta aagaaaaatt ctgaagatgt agtaaaggca tatgtagaat
     9481 ccagtgaacc tgttgttcaa gcagaccaaa gagcaaaaga tatagttgat agagctgaga
     9541 gaatagccaa tgagataaga attgggtcaa ttgaatatgc agaggatgtg ctttctagtg
     9601 ttgagtataa tttaagagaa atattagaag aagtaaaaag agataaagct gaacttagac
     9661 ctaaaaaata aattttaaat aaataaaaat ggagctatca cacaaaaaat tccaatttat
     9721 gtgtgatagc tctcttttaa tatagcaatt ttttgttata taa
//
LOCUS       DAGSHT010000122         9738 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGSHT010000122 DAGSHT010000000
VERSION     DAGSHT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.126"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            419..2497
                     /locus_tag="MJB72_002583"
     CDS             419..2497
                     /locus_tag="MJB72_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBY8263667.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2497..3276
                     /locus_tag="MJB72_002584"
     CDS             2497..3276
                     /locus_tag="MJB72_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8263668.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3269..4078
                     /locus_tag="MJB72_002585"
     CDS             3269..4078
                     /locus_tag="MJB72_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY8263669.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4098..4580
                     /locus_tag="MJB72_002586"
     CDS             4098..4580
                     /locus_tag="MJB72_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8263670.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4601..5005
                     /locus_tag="MJB72_002587"
     CDS             4601..5005
                     /locus_tag="MJB72_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263671.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5161..6321)
                     /locus_tag="MJB72_002588"
     CDS             complement(5161..6321)
                     /locus_tag="MJB72_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBY8263672.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6529..8079
                     /gene="xylB"
                     /locus_tag="MJB72_002589"
     CDS             6529..8079
                     /gene="xylB"
                     /locus_tag="MJB72_002589"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBY8263673.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            8175..9512
                     /gene="xylA"
                     /locus_tag="MJB72_002590"
     CDS             8175..9512
                     /gene="xylA"
                     /locus_tag="MJB72_002590"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBY8263674.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         3701 a         1123 c         1804 g         3110 t
ORIGIN      
        1 tttcataaat tatttataat tataaaatcc attgttttga acaatatcta atatattatt
       61 atctgtattt ataaggtcac tatacatatt ttcaagtccg atattatcta cataataggc
      121 aaactttatt tctgtatgtg ttttaaaaaa actagatgta tataggtatc atcacagata
      181 ttaaaagtat aaataaaaga tattaagaac gactttcaag caacatatta gaaaatttta
      241 ctaaaatccg gaaagcgatt gacaaacaaa gtcaaataaa gtatattatt ggtaagttag
      301 tttaatgtac aaatgaatta tatataaaac ctaatttata aataacgata ctaaacgaca
      361 ttatttaact taagctacac aaagatagaa tattatacca ttgatttagg aggtagccgt
      421 gataaaaata tctgaagact atattgaaaa aagatttgac aatgaactat tgcgaataga
      481 agcatgggga aaaaactctt tacgtattag gtcgtttgta gaccaaaatt ttgttgatga
      541 aaactatgct cttaacgaaa aacctaagtt aaataaagat gatattacaa ttaataaaaa
      601 tgaagatgga agtgcaatca ttaaaaatgg aaagataaaa gctgtcttag accatagaga
      661 tagaattact ttttataatg ataaaaatga aatattattg aaagaatata ttcgattaag
      721 agctgtaaaa catgatgatg gtggagaaga tgtagggaca attgagatta ccaaagactt
      781 taactctact ctaaagttaa aatcaagaga atatcgacca agctatagtg gagaatttga
      841 agtaactact agatttgaat ctgagccttc tgaaaagatt tttggaatgg gacaatatca
      901 acataaattt ttagatttaa agaatactgt tttggagtta gcccaaagaa attctcaaat
      961 atcagttcct ttttatattt ctagtctagg ttatggactt ttatggaata atccgggaat
     1021 tggaaaagta tcttttgcta aaaatatgac agaatggaag atgttttcta caaattttat
     1081 agattattgg attacttgtg gagaaagccc aaaagagtta aataaaaatt attctcaagt
     1141 tactggaact gttcctatga tgcctgaaaa ccttttagga ctatggcaaa gtaaactaag
     1201 atatagaact tcagaagaag ttttagatgt tgtgaaggaa tattctaaaa gaggaattaa
     1261 gctttcatca attgcaattg actattttca ttggcctaag caaggtgaat ataaatttga
     1321 cttagattat tggaaaaatc ctaaagaatt agttaaaaaa ttgaaagaag agtattcagt
     1381 agaaccaata gtatctgtat ggcctacagt tcaaagtgat gcagaaaact acaatgatta
     1441 tttagaaaat ggctatttag tcaatgttaa tcgtggtgtt agaatgacaa tgcaaattca
     1501 aggaaataca gtctttgtag atatgacaaa tgaaaatgca agagaatatg tttgggatag
     1561 aatagataaa aattataaac aacttgggat tgactattat tggttagatg tagctgaacc
     1621 tggctattct gtgtatgatt ttgataacta tagatataaa aaaggaaatg ttttaagttg
     1681 tggtaatatt tatcctattg attatttaaa aatgatttat gatggtttac ataatgacat
     1741 agagtctgtt gtaactcttg taagaggagc ttgggcagga gctcaaaagt atggagcttt
     1801 agtatggtct ggtgacattg attctagttt tgaggctttt aataatcaag taaatacagg
     1861 gttaaatatg ggactggcag gtataccatg gtggactact gatattggag gtttccatgg
     1921 aggaaatcct aaagacccag aatttagaga acttatggta agatggttcc aatatgctac
     1981 tttttcacca attttgagaa tgcatggaga tagattgcct catagtaaac cattatcaaa
     2041 taaaggtgga ggctcaatgg taactggagc tcctaatgaa atatggtctt atggtgaaga
     2101 agttgaagta attcttacaa aattcataaa aattagagag agcttaaaaa cttatttaaa
     2161 aaaactgatg aaagaagcgc atgaagatgg aacaccagtt atgagaactt tattttatga
     2221 atttcctgaa gatgataaaa cttgggaggt ggataataca tatatgcttg gagatgaaat
     2281 tttagtagct ccaataatga attataaaga tagaagtaga aaagtatatt taccaaaagg
     2341 acatacatgg gaaaatatat ttagtggagt tagttatgaa ggtgggaaaa cttatgaagt
     2401 tgaatgccca ctggaagaaa ttcctatttt cctaaagcaa gattccagtt ataattttaa
     2461 agaactaaaa aatattttgg gagaggtgaa atcataatgg agccacaatt atggcaatta
     2521 ttattaatcg tactatatgg attttttata aactatgaaa aaaattcaac aatgtttgga
     2581 acatatcaac cagtaacagc aggttttatt actggtctta tcttaggaga tataaataca
     2641 gggttatata ttggaggtac attacagcta ttatcattag gtattagtaa ctttggtgga
     2701 gcatcaatac ctgattatca aacagcaagt atagtagcca catttataac tattacgact
     2761 aagcaagaag catctgtagg aatctctata ggtattccag ttgctttatt gatggtacaa
     2821 ttagatgttc ttagaaatac tattggtata tggctagttc ataaagcaga agatggagct
     2881 aaaaaaggaa attataaaaa tataacctat atgcaaatgt taggagtact attaacagca
     2941 gctacaacag gtattccagt tgctctatct gtaatatttg gaccttcttt aattaatacc
     3001 attttgaaat atacacctga atggttaaca ggtggtttaa cagtagctgg aggactactt
     3061 ccagcagtag ggataggatt attacttaga tatcttcctg ctaaggagta ttttagttac
     3121 ttagtaattg gttttgtttt agcagtatat atgaaagtac cattattagg tgtagcttta
     3181 attggtggag caattgcact tataatttac aaaaagaatt tggaaaatca agaacaacaa
     3241 tatactgtag taggaggtat ggacgaagat gagtgataag gtatcattaa ataaatcaaa
     3301 tgaaaaggtg aaaaaaagtg tcttatggcg ctggtttttt actagttctg tttcttcaaa
     3361 ttatgagaag atgcaggcct tggcatactg ttatgcagta ctaccttttt taaaagtagt
     3421 atacaaagat aatccagaaa agttacaaag tgctgtatta aatcatttac aattttttaa
     3481 cactaatcct tgggttgccc cttatatttt gggtattaat gtagcaatgg aggagttatc
     3541 aagtgaagag actgaagagg ctgttgcttc aattaaaaca ggcttgatgg gaccagtagc
     3601 aggtttagga gatagtttat ttgtcgttat tccgtggaca atttttggag ctatagcagc
     3661 taatatggcg atagatggaa gtccatttgg aatcttactg tggattgtag tgagtgtagc
     3721 attgaaatta ttaagtatac cactatttaa tgctggttat acatcaggta ctaagctaat
     3781 atcttcaatt gaaaaaagtt taaagatttt aactgagagt acatctattt taggtttaat
     3841 ggttgtaggt gcattaatac cttcagttgt aaaagctaat gttgctttga actttcaaca
     3901 aggtgatttt acaatgaaag gtcaagaaat attagaccaa attatgcctg ggttagttcc
     3961 tgcaatttta gttgtgattg tttattgggc tcttaaaaag aatataaaac ctatatactt
     4021 aattttagga gtaatggttg tttcaataat tttatatgca ttaggtattt taaaataagg
     4081 aaaggatgat attagatatg gaaatgaaag gtattaaaaa tattcgtata gatgaccgtt
     4141 taatccatgg tcaagttgca actatgtgga gtaataaatt gggtgttaca agattaatgg
     4201 tagtaaatga tgcagtagct aacaattctg ttcaaaaaca agttttaaga atggctacac
     4261 ctgcaggtat agcgtcatct ataattacag aagagactgc tataaaaaat attactgcag
     4321 gaaagtatga aggacaaaat gtattattaa ttgttaagtc tcctgtggac cttattccat
     4381 ttgtagaggc aggatttaaa atacataata taaatgtagg aaatatgtca agtagaaaag
     4441 atacaacagt tctaagacct aatatcagtg ttactgaaga agagaaagaa gcatttaaaa
     4501 gacttttaga tgaaggaatt gaaattacaa ctataatgac gcctgatgat aagaaaactt
     4561 atcttgtaga catcttatag atttaaagag gtgtattaaa ttgaatagta ttattttgat
     4621 gagtcatggc aatatggctg aagaagtctt aaattctgca aaaatgataa taggggattc
     4681 tatagattat cctactgtaa acatgaaaca agatgatgga atagaaggaa caatagaaaa
     4741 attaaaaaaa gctttagaag tgtacaagga aagttcagaa ataattataa tggcagattt
     4801 actaggtgga actccatgca atgctgcttt attagtagcc aataatgaca gtcgaattaa
     4861 aattatttca gggctaaatt taggtatggt tattgaatca gcttttttag atacaaagga
     4921 attggcagca aaattagaga aaataggtaa agataatatt tctatggttg aacaaaatgc
     4981 aagtttatta tctgatgatg agtaggaaga ttatattaat atttaaaacg aaatacttat
     5041 gttaatattt aattgatata caatttttaa aaaatatagc taatcttgca taaactatat
     5101 aataaatcta attttagaat gtataattta aagggttatg gctgtatata gtcataaccc
     5161 ttatatttca ttaattaaag ttaaattttg tatattcaaa aagctcttga tatttacggc
     5221 agagccacca tatagagatg cctcagaccc taatgatgaa atttctatat ttattccttt
     5281 gttaaaagag ctaactaaca tatcttttgt aatctgtaat atctcaggga tatcactaat
     5341 tatttggcta tttaaatata ttatttcagg agcataagtt gtaatagcat tatttatagc
     5401 tattgttaag taactacaaa actcatgaat aacttttttg gcattctggt tatcttcata
     5461 atatagttgc tttactatat cagaatctat tttaggaata ttttctaaag aagaaagctg
     5521 ttcaaatacc tttttttctg aacaatactg ttctaaacag ccacgatttc cacaaggaca
     5581 tagtttaccg ttaggcatga tgatagtgtg accaatttct ccactcatac catttctgcc
     5641 actatataat ttattattta ttataatgcc tgaaccaaat ccactgtgaa tactgagact
     5701 aagtagtgag ttatgtacag ttgaaaaagt attctcagct aaagctgtta agtttgcttc
     5761 attttctatg tgaataggaa agtcatactt tttgctaagg atgctatata agtcaatctc
     5821 attaagattg taatatggag taaacaatac tttattttca caagtaatcc catgtatagc
     5881 caaagtaaga ccaattacct tataaggagt gtctattttg gatatgttat aactatttat
     5941 aatttcatct atcaattgta taacattgtc tttacttact tgtatatctg ttaatttctt
     6001 agaatttata atagttccat ctaagtaaga tagagaagaa aatatatagt cgtatccaat
     6061 gtccatactt aaagaaatcc ctgcacattt attaaatact aataatatgg gttttcttcc
     6121 gccactatga gtactatttc caattccgat ttcatgaact agagattcat caataagttt
     6181 tttggtaata gcggatatag ttgctttatt caatcctatg gtagaagcta tacttgccct
     6241 agaaatagga ccatttttta taatttgttc aagtaccaat ctttcattca tttctcgaat
     6301 agtgtattta tcagtaacca atttgatatt cctccttaaa ataatattgt aatacttttt
     6361 acacaaaaat aaaaggttat tttgcattga caaagataat taaatatttt attattagtt
     6421 cataagttag tttaatatac taacaaaaat aaagcaagta aaatatacct aaaatataaa
     6481 aaaattagga taggaaaacg atagttatga agtggcattc aaggagggat gaagttggag
     6541 tatgtattag gtgtggatat tggtacatca ggtacgaaaa cagttttatt tgataaacta
     6601 ggaaatacta taaaaagttg tacatatgaa tatcctctta tacaagagaa atcaggttgg
     6661 gcagagcagg atgcaaatga ttggtggaaa gctgtagtcg aatctataag agaagtagtt
     6721 caaagttcaa atataagcag tgaatgtata aaaggaattg gattatcagg acagatgcat
     6781 ggtctagtta tgctggacaa tgaaggtaaa acattgagaa actctataat atggtgtgac
     6841 caacgaacag taaaagagtg tgaggagata actgatttag taggtgaaga aagactgatt
     6901 gaaataactg ctaatccagc acttacagga tttacagcat caaagatatt atgggttaga
     6961 aacaatgaac cagatatata taaaaatact aataaaatac ttcttccaaa agattatata
     7021 agatacaaat tgacaggtga atatgctaca gaggtatcag atgcaagtgg tatgcagttg
     7081 ctcgatataa gaaaaagaga ttggagcgat gaagtactag aaaagttaaa tatagataaa
     7141 aatcttttag gtaaagttta tgaatcacaa gaaattacag gttatgtgac tagagatgta
     7201 gcaagtttga cagggcttaa agaaggaaca atagtggtag gaggagctgg agaccaagca
     7261 gcaggagcta taggaaatgg aatagttaaa gatggagtag tttcatctac tataggtaca
     7321 tcaggtgtag tatttgctta tacaaaagag ccaaaaattg ataaagaagg tagagttcat
     7381 actttctgtc atgcaatacc aaatacatgg catgttatgg gagttactca aggagctggc
     7441 ttgtcattaa aatggtttaa agataatttc tgtcaaagtg aagtggaagt ttcaaattct
     7501 ttaggtgaag atgtatatga gattataaat aatcaagtaa gtaaagtacc aacaggatgt
     7561 aatggattgc tttacttacc atatatgatg ggagaaagaa ctcctcatct tgacccttat
     7621 gcaagaggtg tgttctttgg attatctcca atccattcta aaaaagaaat cgcaaggtca
     7681 ataatggaag gtgtttcata tagtcttaaa gactgtatgg atataataga gaatcttaat
     7741 atagacgtta atgaagtaag agcctcaggt ggaggtggaa aaagtaaagt atggagacag
     7801 atgcaagcag atatgtttaa tcaagatgta tatactataa attcaagtga aggaccagca
     7861 cttggagttg caattcttgc attagtagga gctggaatat atgaaaatat acaaaaagct
     7921 tgtgatgcta taataaaaac ttcaactaaa ttagaaccaa taagtgaaaa tgtagataca
     7981 tacaatatat atcatagatt gtataaaaga atatacaaat ctttgaaaga tgactttaag
     8041 ttattagatg aagtagttaa tgcaaaaata aataattaaa ttagcttaaa aagaaaaata
     8101 aagatagaaa atagttttaa attaagaaaa catactacat atattatatg tgtaaaatat
     8161 taggaggaca tgaaatgagt gaaatattta aaggaatagg acagataaaa tttgaaggag
     8221 taaaatctga caatgaatta gcatttagat actacaatcc tgaacaagtg gtaggtaata
     8281 agactatgaa agaacactta agatttgcaa tgagctattg gcatacttta tgtggagagg
     8341 gtaatgaccc atttggagta gggacagtag agagaccttg gaataatata acagacccaa
     8401 ttgagatagc aaagattaaa gttgatgcag gatttgaatt tatgtctaag atgggaattg
     8461 agtatttctg ttttcatgac agagatatag caccagaagg aagagattta gaagagacaa
     8521 ataagatatt agatgaaata gttgagtata taaaagttaa tatggaaaag acaggtataa
     8581 agttattatg gggaactgcc aatatgtttg gaaatccaag atttgttcat ggtgcatcta
     8641 caacatgtaa tgcagatgta tatgcatatg cagcagctca ggttaaaaaa gctatggaaa
     8701 taactaagta cttaggagga gaaaattttg tattctgggg tggaagagaa ggttatgaaa
     8761 ctttacttaa cactaataca gaacttgaaa tggataactt tgcaagattc ttacaaatgg
     8821 ctgtagatta tgctaaggaa ataggattca ctggacaatt cttaatagag cctaagccaa
     8881 aagagcctac taaacatcaa tacgattttg atactgctac tgttttagga tttttaagaa
     8941 agtataatct ggataaatac ttcaaaatga atatagaagc aaaccatgca acacttgcag
     9001 gacatacttt ccaacatgaa ttaaatatag caagaataaa caatgtttta ggtagtatag
     9061 atgcaaatca aggagaccta ttattaggtt gggatacaga ccaatttcca acaaatatat
     9121 atgatgcaac acttgctatg tatgaagtgt taaagcaagg tggaatagca ccaggaggat
     9181 ttaactttga ctctaaagta agaagagcat cttttgaggt agaagactta ttcctagctt
     9241 atattgcagg aatggataca tttgcaaagg gattattaat agctcataaa ttattagaag
     9301 atgaggtatt tgagaacttt actaaagaaa gatatgcaag ttttagtgaa ggtataggta
     9361 aggatatagt agaaggtaag gttggattta aagagttaga atcttatgca ctgcaaatgc
     9421 cagttataaa gaataagtct ggaagacaag aaatgctaga agctatactt aacagatata
     9481 tatatgaagt agatacaatt tctaataagt aattgtgtag taattaaact tggatgtttg
     9541 aataaaataa caggatatgt tcttgttttt attctaaaac ggacaaagat aagtatttgt
     9601 aaaaattctc ataatcatga taaattaagg atatagactt aagtataatt agtttatatc
     9661 cttaaaattt ctataaaaaa taatcatttc tatagttatc aatattttta attatcaata
     9721 tctttctcaa ctaataaa
//
LOCUS       DAGSHT010000123         9689 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGSHT010000123 DAGSHT010000000
VERSION     DAGSHT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.127"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..557
                     /locus_tag="MJB72_002591"
     CDS             <1..557
                     /locus_tag="MJB72_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263675.1"
                     /translation="NNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMARIKAIQLI
                     DKYIDLKSEDYDEESILLNVLNVLYDVYMEDRIVENEGINSIKKSILSILGEDSKLNE
                     DNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQITVMSKT
                     FNDNILSIKINSKSGIYILKFKKV"
     gene            complement(562..768)
                     /locus_tag="MJB72_002592"
     CDS             complement(562..768)
                     /locus_tag="MJB72_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263676.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            904..2658
                     /locus_tag="MJB72_002593"
     CDS             904..2658
                     /locus_tag="MJB72_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBY8263677.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRVYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            2676..3818
                     /locus_tag="MJB72_002594"
     CDS             2676..3818
                     /locus_tag="MJB72_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY8263678.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(3990..4913)
                     /locus_tag="MJB72_002595"
     CDS             complement(3990..4913)
                     /locus_tag="MJB72_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8263679.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            5139..5717
                     /locus_tag="MJB72_002596"
     CDS             5139..5717
                     /locus_tag="MJB72_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY8263680.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            5833..6744
                     /locus_tag="MJB72_002597"
     CDS             5833..6744
                     /locus_tag="MJB72_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBY8263681.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            7351..9270
                     /gene="cooS"
                     /locus_tag="MJB72_002598"
     CDS             7351..9270
                     /gene="cooS"
                     /locus_tag="MJB72_002598"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBY8263682.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            9590..>9689
                     /locus_tag="MJB72_002599"
     CDS             9590..>9689
                     /locus_tag="MJB72_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8263683.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVA"
BASE COUNT         3759 a         1074 c         1786 g         3070 t
ORIGIN      
        1 taaataatag taaaatagag tatgtcgaat tactacagag tattaaagac aagataatag
       61 gattttctat agaattagac aaatcagatg ttgtaaagtt tcagatggca aggattaaag
      121 caatacaatt aattgataag tacattgatt tgaaatctga agattatgat gaagaaagta
      181 ttttattaaa tgttttaaat gttctatatg atgtatatat ggaagataga attgtagaaa
      241 atgaaggaat taatagcata aaaaaatcta tattatccat tttgggtgaa gatagtaaat
      301 taaatgaaga taatatagat tttatttttt ctatgtccga atatgttgtt aaattgagaa
      361 aatataaaat tggagttaag gcatataata aaagcataga cccaagaagt cttataagac
      421 ttgaagaagg taatactata gtagacccta tatttaatca aataacagtt atgtcgaaaa
      481 cctttaatga taatatatta agcataaaaa taaattctaa atcaggtatt tatatattaa
      541 agtttaaaaa ggtttaggtt attagagtgt gctatttttt tctagataat ctaaaaaatc
      601 tttttcctta gtgtcagtta aaaaacataa ttcctttact atattattcc taaggtcaat
      661 gaaatcacta atttgagtat tttcattagt gtattcaaga gactttataa taagtagtaa
      721 atctcttttt gataaagagt tgatagatac tttttcaatt aattccataa aaaacttcct
      781 ttcctcacat tacttgaaat taataaatta attatataga tattatttag actttacata
      841 tactatttta acctataagg ggtacaaaaa gacagtataa tcttaaaaag aggtgagaaa
      901 tatttgaaaa ttttattaac aacattaaat tccaagttta ttcatacaaa tttggctata
      961 agatatctaa aagaatttgt aagagatttg atagaagtta atatgaaaga atacacgata
     1021 aataacgatt tggactatat tttaaaggat atatataaaa atgaatatga tataatatta
     1081 ttctcaacat atatctggaa tgtgggagat atagttaaac tttgtgataa tctaaaaaaa
     1141 attagaccta atactaaaat agctttgggc ggaccggaag tgagttatga tagttatgag
     1201 gcaatgaaaa agtatgattt tgtggattat atactttatg gagagggaga attaattttt
     1261 agagacttag tacttcactt gcaaggaaag atggaaataa atgatgtaaa tggacttgta
     1321 tatagacaag gcaatgagat aatagtgaat aagcctatgg aattacttca agatttggat
     1381 gagattccta gtccttatga aaacttgaat ccaaaagaat atgaaaatag aatagtgtat
     1441 tatgaatcat caagaggttg cccttttaac tgccaatact gtttatcatc tactattcca
     1501 ggtctaaggt attttagttt agacaggata aagagtgatt taaaagcttt aatagatgca
     1561 agagtatcac agataaagtt tatagatagg acattcaatg caaatagaaa agtggctatg
     1621 gaaatcatgg atttccttat gaaaaatgat aatggatata caacttatca ttttgaagtt
     1681 acagcgtatt tgatagatga taagatgctt gagtttttgg ctgattgtaa agaaggtctt
     1741 tttcaatttg aaataggggt tcagtcaacg aatgaaaaaa cacttgatgc agtaggtaga
     1801 agggatgatt ttaaaaagtt gtctcatgtt gtacaaactg tagcatctta tagaaatata
     1861 catcagcact tagacttaat tgcaggactg ccttatgaag attataagag ctttgaaaat
     1921 tcttttaatg atgtatttaa tctaggtata gagcatctgc aattaggctt tttaaagatg
     1981 attaaaggga caggtatgag aaaagttgca gatgagcatg gttttaaata taaggattat
     2041 gctccatatg agtttttata caataattat ataagctatg aagaaacttt gaagctaaag
     2101 gatatagaag atatactgga gagatactac aattctaaaa actttgtgtt atctatgaga
     2161 tatattatag gcagagttta taaaaaaagc ccttttaaat tctttgaggt ttttgccaag
     2221 tattttgatg aaaatggcta ctttgatttg gcacaaggaa agaatcagtt atataaaata
     2281 ttgatggatt tctataatga aaaaatcaac atagataatg atgtattcaa cgatatatta
     2341 aaatatgact atatatcact aggtaaaaca tcaaatatac cacagttttt taataagcta
     2401 gatgtggatg attttaagaa tagatgccat gtatttttac aagataatga taacttatca
     2461 acatatttac ctagttttgt tgataaacca gctaaacata taataaaata tgttcatttt
     2521 gagccattta gatttaatgt tgtggattta aaaaatgata taaatacaga aataagagaa
     2581 gtagaaaatg tagtcttatt tgagtatgat gataaaaaaa tatttgagaa atctaaaact
     2641 cataaagtag agatttaagt atttaggagg ataaaatgga ttttaagata gaaaaatatt
     2701 tgctaaaaaa agcagaagaa ttagctttta taactataaa acatggtgga gagtttaaat
     2761 taaaaagcta tgaagtgcca aaaggtggat tagatgtacc tataaaaaat gaggtattag
     2821 taaaagggat aaaagaaaag actgctcaag ataagttaaa ttcaatgtct atagcagatg
     2881 caatgatata tataattgga atagatagta agtttaaaaa caatgcagag tatgagaagt
     2941 ttttaaatgc tttatcaaaa gatattgatt tggatttgaa atcgtatatg ggatatatgt
     3001 cgagaaaata ctttgagatt ggtgaacata cagattcttt aatatatata aaagcattta
     3061 taacaatgta tccagatgat ttagatgcaa tgtataacta tgcgattgtt tgccaagaga
     3121 tagctaaaca gtatcaaaaa gatatggatg ataaggcaat gaatgcattc ttacttgaag
     3181 ccatggctaa gcttgaaaag gtaattgatg ttgatgagaa ttttgcatta ggttattatc
     3241 acttaggcta ccattattat aatcaaggtc aatatttaaa gactaagctg acttgggaag
     3301 aagcgttaag gttagggtta gatgctgatt tagtagctga ggtacaagag aatctgggca
     3361 agatggactt taaagttcaa tatgaagaag gatatacact tgtattccaa ggtaagttta
     3421 aggaaggatt agagaagtta cttccattag aagaagaaca tatggattgg tggaacttgc
     3481 tgtttatgat agcacttgga tataagggta tgggcgaaat agagcaggct aagatgtatc
     3541 ttgagaagat attgataata aagcctaatc aggtggatac tatagtggaa ttggggcttt
     3601 gtgaggctta taagaataat ttagataaag caatagagta ttttgaacaa gcggctaaga
     3661 taaaggaaga cccagaaata ctttgcaatt taggaatggc ttatttaaat aatggtgata
     3721 ttgatgatgc aacttactat atagaaagag cttatgaatt aaatccacaa gatgaaatta
     3781 ctattgcttg tctaagagaa cttggaataa ataagtaagg atattgttaa gttgtattta
     3841 attttattgg ttaagtgggg ctatctcaaa atagagataa gcctcttttt tagtgctgaa
     3901 atgatgaaca tatattttac tttgtataaa aaagagtgcc ttatacagtg caaagttcat
     3961 tttgagacac tctcgctttt tattatgatt tatttttctt taggtgtatc caacttagat
     4021 gtatccaaca tgatagaaga aaaatctttt tgtatttgaa gtaaaaattt ttctgctgcc
     4081 tttgaaaata tttgatgttt tttccatata atatttaatt tagcttctaa aggaggagat
     4141 agaggtctaa aacaaagatt actgttttct gtagtattga cgagtctatc taaacacaga
     4201 gcgtagccca gaccttcttc taccaaaaga gaagcgttat aaactaaatt gtatgttgca
     4261 ataatgttta gtttttcatt actgtcttga agccatttag aaattgcttt atcaacaagt
     4321 gtctgtcttg aaataataag aggtatattg cataaatctt ttggatttat tgtgctttgt
     4381 gatgcaaggg cactatcttt acgcattagt aaaccccata catcagtagc aggaatttgt
     4441 gcataatcat acttctgtat atttgcaggt tcaattaaaa taccaaaatc aagcagtccc
     4501 ttgtctaatt tttcagtcac atcatcagca ttcccactaa aaaggtgata tcttacattg
     4561 gggtattttt gatgaaaatg atgggcagtt ctagctataa gtcgcatagc ttcagtttca
     4621 ccacttccta tatatatatc accactaata atctcattgg gattgtcaaa ttctaattca
     4681 accttatcaa ctaatgatat aatttcttct gctcttttat gtaaaagcct accttcattt
     4741 gttaaggtga tttttctatt tcctcgaacc aataatttaa ctcccaactc ttcttcaagt
     4801 tccataattt gtctagaaag tgttggttgt gttatatgta aaaaggttgc cgcaccagat
     4861 atactttctt ctcttgcaat tgctaaaaaa tatcttaata ctcttatttc cataattatc
     4921 tctcctcaat attaacttat ttattatata attatatcaa tttaaatgct ttttaagtat
     4981 agttaagtat ttaatataga tatttgatat agatataatt gtatattaca ctaaaagaag
     5041 caatgatatt ttaataaaaa taaattaaat taatttttat taaaatatta tttagaatgt
     5101 acaaataaat taaagcacat attgaaagga tgtataaaat gaatatcaat gaatttttaa
     5161 cacatatcaa tcaaagaaaa cctgtttcta gtaaatcaga aatacatgaa tttatggtta
     5221 agttgagtaa tgaagcaatg aaaatcacaa gtaagttaaa ttcttcttat catgaaccag
     5281 aagaagtccg tagtttattt tctgagttaa ttggaaaaaa ggtagatgaa tcatttttta
     5341 tgtttccacc attttataca gattgtggaa aaaacatcac aataggaaaa aatgttttta
     5401 taaatagcag ttgccatttt caagaccagg gggggattga gattggagat aatacgcaaa
     5461 ttgggcataa tgtggtattg gctacactta accatggtat tgcaccagag aaacgtggta
     5521 caacttatcc ttctccaatc acaataggaa aaaatgtatg gattggtgct aatgtaactg
     5581 tagttccagg agttactatt ggagataatg caattatcgc tgctggagca gttgtaacaa
     5641 aaaatgttgc agaaaatact attgtaggtg gtgttcctgc aaagctttta agagctattg
     5701 atgaaatccc agaataaaat attaaactgt cttttagtta tataaattct ttataacata
     5761 aaaaataaat tgagaaaagg tatagcaaat atggtattat ttagttaaat atatataagg
     5821 gaggggttac ttttgataaa aaaattagat gatattttag aacaacttaa gggaggagag
     5881 aaaattgtac tctctgtagc agcagctcat gataaagaag ttttaatggc aataaaggat
     5941 gctgtagaaa gaaatataat tactcctata ttggttggaa atgaaggcaa aataagagaa
     6001 atatcaaaag aaataggatt tgatttaagt ggtatcaaaa tagtggataa ggatgatatc
     6061 aaagagtgtg cagaaatagc agttaaatta gtatcaagta aagaagccga ctttgtaatg
     6121 aaaggattat tagatacatc tgtcatatta aaagaagtgt taaataaaga ctatggtctt
     6181 agaactgata gtttattaag tcatgttatg gtatatgagt tagaaaaata ccataaatta
     6241 cttataacta cagatggagg tatgaatata gctcctgatt atgaacaaaa ggcaaagata
     6301 ttaaaaaatt ctataaaagc tgcaaaagct ttaggaatgg aaactgtaaa agttgcatgt
     6361 ttggcagcta aagagaaagt aaatcctaag atgcaagcaa ctgtagatgc agatatgtta
     6421 gcaaaagctt gtaaagaagg agaatttggt gaaaatgtaa ttgtagaagg tcctctggct
     6481 tttgatttag ctgtttcaaa agaagctagt gagataaaag ggtttaaaag tgaagtatct
     6541 ggagatgtag atatcatatt ggttccaaca atagaggttg gtaatggaat agggaaagcc
     6601 tttacatata tggcagactc taaatcagca ggtataatta tgggtgcaaa agctcctata
     6661 gttttagtgt caagagctga ttcacatgaa tctaaattat attctattgc atatggggct
     6721 attgtagcta aaaatatgaa ataggataaa tcgcttgaaa taaatgaatt aaagtagaaa
     6781 aaatagtcaa aatgtctaaa ttaatgctgt attaaaaaag ttttatataa aaaataatta
     6841 tagcttcaaa tgaatatata caaatgttca atttgaagct ataattaata ttaaagtagg
     6901 aaaaataaaa aaattcaaaa aaattagtcc aaaaaatgct attctaagaa atttttacta
     6961 attaagttta gggagtaaaa atctgtaata agtccaaaca gtagtgaaag ataataaata
     7021 cacaaacaat ctgcaataat ataaaaatga ctagaaaaat ttcaaatttg atttaaaaaa
     7081 atcaaaacat aatttaatta aactgaagta tcaaaaaagt aggtttagat attaacaaat
     7141 agccaaaaac aagcgataaa atcaacttaa attaagtgta gaaaataaaa tttttatgta
     7201 ttatctaatt ttgttaaaaa aatatttaaa tctaaagcaa agcactgaaa attagtatat
     7261 aatcaactta gaaagcattt attttattat tttatgacat gaagtcagtc acctggggga
     7321 ttttaaaaat caaaaaaagg ggataggaat atggatgaaa aaatgttaac aattgattta
     7381 aacagccaga agatgatagc taaagctaga gaagaaggcg tagaaacaat gtatgataga
     7441 aaagaaggct ttaaggcaca atgtggtttt ggtcttcaag gtgtttgctg tagaatatgt
     7501 ggaatgggac catgtagaat aagccctaag actccaaggg ggttatgtgg agcagatgag
     7561 catactatag taggtagaaa ctttgcaaga atggttgctg gtgggacagc tgcccattca
     7621 gaccatgcta gagatatagc acatacatta gcattagctg atccaaatgg aaactataaa
     7681 ataagagacg aagctaagtt aattacttta gctaaagaat gggatgtaga gacagaaggt
     7741 agagatatct atgatgtagc acatgaagtg gcagaaattg cattaatgga atttggaaaa
     7801 ccttttggga cattaagatt tataaaaaat gcaccagaac caagacaaaa aatatggaaa
     7861 gaatacgcta tagagccaag agctatagat agagaaattg caacaataat gcactctact
     7921 catataggat gtactggtga tatagatagt ttaatacaca tgagtcttag aacttctatg
     7981 gctgatggtt ggggtggttc tatgataggt actcgtctaa gcgatatctt atttggaact
     8041 ccagtgccaa gaagaacaga ggcgaattta gcagtattag aagaaaataa agttaacata
     8101 atattacatg gacatgagcc agcattatca gaaatgatag ttttagcatc agaagagcca
     8161 gacttagtag ctttagctaa agaagttggt gctgatggaa taaacttagc aggtatgtgt
     8221 tgtacaggta atgagattac aatgagacat ggtgtaaaaa tagcaggaga cttccatcaa
     8281 caagaacttg caataataac tggagcagtt gaagctgtaa tagttgacgt tcaatgtata
     8341 ttccctgcat tagctagagt tgcagattgt taccatacta aatttgtaac aacttctcca
     8401 aaagctaaga taacaggttc aacttatatt gaatttagag aagaacaagc acttgatgat
     8461 gctaaagcaa tagttaaaga agctatttta aactttaaaa atagagataa atcaaaagta
     8521 cttatacctg aattaaaatc aggggcaaca gttgggtata gtgtagaagc agtagtaaat
     8581 caattagaca gagttgtaaa ctctcatata gacccagcag gaacagttaa gccattaaca
     8641 gactgtctaa aagctggagt attaagagga gcagctggtg tagttggatg taacaatgct
     8701 aaaggagttt caaatgaggc tcacgtaaca ataatgaaag aattaatcaa aaacgacata
     8761 atagtagtta ctacaggttg tggagcttca gcagcagcta agtttggttt aatggaatct
     8821 gatgcggctg aaaaatatgc aggtaaaggt cttgcaactg tctgtaaatt agtaggaatc
     8881 ccaccagtac tacatatggg ttcttgtgtt gatataagcc gtatactaga tttagttgga
     8941 gcagcagcta attacttaga tatggatatg tgtgaccttc cagttgtagg tatagctcca
     9001 gagtggatgt cagaaaaagc agttgctata ggatgctatg ttgtagcttc tggtatagac
     9061 acttatttag gaataatgcc tccaatagct ggttcttcaa gagcagtgga tatattaact
     9121 agtgaattaa aagataaagt tggagctaca tttactgtaa atacaaatcc aaaagagtta
     9181 gcagctacaa taatagaaga tatagagaaa aaacgtgttc actttgaagc attagttgaa
     9241 gaaaaaatgg cagaaaaggc tgaagcttaa aaaatagtga agttatagaa taaacgtttt
     9301 tataagaagt ttctggttga ataaagttga ataaagtcaa atattaaagt aaagaacatc
     9361 aaaatataat gaatatactg aataaataaa atataaacaa tattaaacaa tattaaagaa
     9421 taataaaact atacatgctg ctttgaaaag ctagtagggt aaatttaatt ggttcctgta
     9481 aggactagct gtagtttatt tttagtgtat aaaaatatag agtataaaaa atatttaact
     9541 gtaaaattaa ctactaaaaa gcaaatagta ataaaaggtg gtagggatta tgaagatagc
     9601 aataacaggt aaaggtggag taggtaaaac gactttttct tcaatgctct ctaggatgtt
     9661 tgctgaagac ggatacagag ttgttgctg
//
LOCUS       DAGSHT010000124         9650 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.128, whole genome shotgun sequence.
ACCESSION   DAGSHT010000124 DAGSHT010000000
VERSION     DAGSHT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.128"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            245..910
                     /locus_tag="MJB72_002600"
     CDS             245..910
                     /locus_tag="MJB72_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBY8263684.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            932..1864
                     /locus_tag="MJB72_002601"
     CDS             932..1864
                     /locus_tag="MJB72_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBY8263685.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1885..2526
                     /gene="pcp"
                     /locus_tag="MJB72_002602"
     CDS             1885..2526
                     /gene="pcp"
                     /locus_tag="MJB72_002602"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBY8263686.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2646..2978)
                     /locus_tag="MJB72_002603"
     CDS             complement(2646..2978)
                     /locus_tag="MJB72_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263687.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3244..3864
                     /locus_tag="MJB72_002604"
     CDS             3244..3864
                     /locus_tag="MJB72_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY8263688.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(4189..5013)
                     /locus_tag="MJB72_002605"
     CDS             complement(4189..5013)
                     /locus_tag="MJB72_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBY8263689.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(5024..6427)
                     /locus_tag="MJB72_002606"
     CDS             complement(5024..6427)
                     /locus_tag="MJB72_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263690.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(6431..7156)
                     /locus_tag="MJB72_002607"
     CDS             complement(6431..7156)
                     /locus_tag="MJB72_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263691.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(7268..8041)
                     /locus_tag="MJB72_002608"
     CDS             complement(7268..8041)
                     /locus_tag="MJB72_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263692.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIVFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(8047..8934)
                     /locus_tag="MJB72_002609"
     CDS             complement(8047..8934)
                     /locus_tag="MJB72_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263693.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(8948..>9650)
                     /locus_tag="MJB72_002610"
     CDS             complement(8948..>9650)
                     /locus_tag="MJB72_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263694.1"
                     /translation="FWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILS
                     SKEKKKALKSKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVAL
                     KTNLSITKYVILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVI
                     HILPKKLLSVVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGA
                     MSLCFLVIKSDYISR"
BASE COUNT         3449 a         1469 c         1131 g         3601 t
ORIGIN      
        1 aatattgaca tatacttaaa ctatagactt acaaaatcaa ttcatattag cttaaaatat
       61 aatctttgtt attcaaattc ctatacacaa ccattattta ctttgatact ttaaaacatc
      121 ttccctattt tttattaaat tttataataa tagtcctatt aacgcaaata ctataaaaag
      181 agtttaactt tatccattaa ataaatttaa attaaaacat atagaaatgg aaggagaaca
      241 atttatgata aaactaatcg gtatattgat agttgtaatt ggatttatac ttaaaattga
      301 cacactattt actgtattat tagcaggagt ggcaacagga attgttgcag gattagattt
      361 taatcaaatt tttacaattt taggagattc ttttgtctca aacagaggtg tatcattatt
      421 catattaaca ttgccagtga tcggtgtatt agaaagatat ggtttaaaac aaagagctgt
      481 ttctttaata gaaaaattaa aaagacttac aacaggaaaa gtacttacta tatacatgat
      541 agctagacaa atagctggtg ctctatcaat aagaatgagt ggacatcctc aatttgtaag
      601 accacttgtt aatcctatgg cacaagctgc tggtttaagt aatagtgatg agctaaaaga
      661 atcggatgaa gaagccataa aggcattatc tgctgcatca gaaaactatg gaaattttta
      721 tggtcaaaac ttatttgcag gtagtagtgg agtattactt atagcatcta ctctgactca
      781 atttggatac aatgtaactg gacttaatat tgtaaaggct agtactatta tggcagtaat
      841 agcacttgta gtggctacaa tccagtttac cttatatgat aaaaaattaa acaaaagtca
      901 taaaaaatag ttttatttag gaggaataaa aatgcaaaat tttataaaca tatcattaga
      961 aatcttttac gcactaatgg gatttttaat gattgttata gcatataagt cttttactac
     1021 cataaataac aataagaaat atggtacatc attattttgg atacttatat cactgccatt
     1081 catatttggt agattgattc cagccaatat aataggaata atattaatat tatctagttt
     1141 attaacactt tctaaacaag ttgtatttgc aaagtatgaa gaaccagatg aaaattttgg
     1201 aaaagaacaa gcagacaaac ttaaaaataa aatatttata ccatcattaa tattagcatt
     1261 tgctgcagta gtagtagcta tgagcctttc taattttgat aattcttctc aatttgcaat
     1321 aggtgtgggt tcaataattg cattaatatc agcccttata ataactaagg caaaacctgc
     1381 tacatcagtt caagatggct caagattact tcaacaaatg ggacctgcta gtatgttacc
     1441 acaactttta gttgcattgg gagctttatt tactcaagcc ggagtcggtg aagttatttc
     1501 aaccatgata tctggtgtag tccctgctga tagcagatta tttggtgtta tagcttatgt
     1561 attaggtatg gttatattta ccatgataat gggaaatgca tttgccgcat tttcagtcat
     1621 aacggcagga attggtattc cttttgtatt gtctcaaggt ggaaatcctg ctataattgg
     1681 agcattagca ttaacagcag gatattgtgg aactctactt actccaatgg ctgctaattt
     1741 taatatagtt ccagcagcct tgctagaatg taaaaatgat tatattgtta taaaatatca
     1801 agctccagtt gctttggtat taattatagc ccacatatta gtaatgtact ttttaggatt
     1861 ttaatataat taggaggtaa aataatgaaa attttgttaa caggatttga cccatttggt
     1921 ggagaaccaa taaacccagc tcaagaagca gttgaaaggg tcaataataa tataaatggt
     1981 gcagaaatca taaagataac tataccaaca gttatgacaa aatctgttga agctatagat
     2041 aaagctattc aagagcacaa tccagatata gtaatatcag ttggacaagc tggaggtaga
     2101 tttgacatta cacctgagag agttgctata aatatagatg attttagaat aaaagataat
     2161 gagggtaacc aggttataga tactattata aaagaagatg gtgaacctgc atatttttca
     2221 aaattgcctg taaaagctat ggtaaaacat atgaatgaaa ataaaattcc agctagtgtt
     2281 tcaaacactg caggaacatt tgtttgcaat catgtgatgt atggaattct ttatatgata
     2341 gacaagaaat atcctaatat aaggggtgga tttatacaca tcccatatac aacttcacaa
     2401 gtaatagata aaaaaaatac accattcatg tctttagaag aaatagtaaa aggcttagaa
     2461 ttagctatag aagcttgtat tatatataaa gaagatgtaa aagaaattgg tggtgaaatt
     2521 tcataataaa ttttttagta tttttataaa ttaaaaatac taactattaa tttaaatata
     2581 gactgtgagt agattattca aacaatttta ctcacagtct ttttatggta tctacagata
     2641 caaaattaag gtctattcct ctctccccac tcacacattg catctaaaat tggaatcaga
     2701 gattttcccc tttctgatag actatattct accttaggag gtatttgagg atattcagta
     2761 cgaataatca agttatctgc ttctaattct tttaatgata aactcaaagt tttataggag
     2821 atagtattta tataacgttt taatgcatta tatcttacaa cttcaaattc tacaagacag
     2881 taaagaatta tcatcttata tttaccacta atcagtgaaa gtgtataact aaatcctgtt
     2941 tcacttaaat ctgtatttga tatacagtct atactcatat attacactct ccttttggtt
     3001 agtattgaac aaaaatgtgc gtacttgttt ttagaataat ttataataaa attatagtgt
     3061 aagaataatt gaaagtcaat tttataatag gaggtgtatt tactaaaaaa aaagttattt
     3121 taaataggat tctaagatta gacatttcta tataaattga taatattcat gaaaatccta
     3181 ttttataaaa aataatatct cttagaaaat ctattaaata aaattcatgg gaggaaattt
     3241 ataatgaaag ttttattgat aaacggtagc ccaaaccaat atggatgtac ttatacagca
     3301 ctaaatgaga ttactaaagt attgagtaaa cacgatatta aaacagaaat actgtattta
     3361 ggtaaagaaa caatcccagg atgtattagt tgcgcatcct gttttgaaac tggaaaatgt
     3421 attcgaaatg ataaagttaa tgaattgatt gaagatcttg ataatattga tggtattatt
     3481 attggttctc ctgtttactt ttctagtgca actggtcaat taacttcttt tcttgaccgt
     3541 ctatttttca tagctggaag tcgcatggct acaaaattag gtgcttcagt tgtgtcatgt
     3601 agacgtggtg gtgcaagtgc aacatttgac caactaaata aatatttttc aatcagcaat
     3661 atgcctattg tatcatcaca gtattggaat caggtacatg gctttactcc agaggatgtt
     3721 atgaaggatg aagaaggatt acagactatg cgaactctag gagaaaatat ggcatggctt
     3781 cttaagtgta tatcagcagg taaaaaagct ggaattaaag aaccacaata tgaagaaaca
     3841 attatgacta attttataaa ataaataaaa aatgcactaa tattaattaa aacaaaacac
     3901 taatttataa ataaaataag cataaatgta ggataaaaag cactaaaaca gaggtattga
     3961 attttatatc aaacctctgt tttattatta cattaataac caaaccatgt ataacatcat
     4021 atatttctaa ataaattata atattatatt ctaatgttat tgaaattaca ataactgaat
     4081 ttctgctcat aaatttaaat attctacaaa tctcatctaa ataacaattt taatagacct
     4141 aaaacaatcc tcatattgtt cattttgtat taaaaatatc acttacaatc aaaaaccatt
     4201 tattaagtat attcctaaga tatacaatat atatacatgg attggatagt ataaataaaa
     4261 taaatacttc atactcttcc ctttttctcc attgtaaagt aacattaaag gtagagaagc
     4321 aatcatcatc cattggtaat tttgtaaaat agaatttttt aaactataat ctccatttga
     4381 cattgttgta aatataaata tacacattaa aacataacaa atactcaaac tgaacttttt
     4441 ccctctacac aaataaaaaa taatcccaag tataatccaa attggtccac cttctactaa
     4501 aaatggtact ggtaccaaaa tcataaataa agcaataact ctgttagtga ctttaaaaag
     4561 taaagctact gtaaatattc ctaaaattat aggaatgatt atcatcaata atcctataat
     4621 aaaatattta atttctttct cttttctaaa tttctttata aactctattg cttgtaaata
     4681 aatcgctatt aaaaataatg ttgaaaaaat gttatttata attatagctc cttctggatg
     4741 tgaaaataca ttattaatta tttgatttcc tatagccata ataacagacc aaatatacaa
     4801 tctacttata tatttttttc tgttactagt gtgataaaat ccttcaacag ccatgaatat
     4861 aaacagtggt gctgatattc tgcctaccca atgaaaccaa attggtattt gcattgtttg
     4921 tggcataaat gctgctatat ggtcaaatgt cataaatatt aatgctaaaa tcttaatagt
     4981 aaacccactt ataccttttt taaaaaaatc catatcatat ctcctaattt tgttttatct
     5041 ctataataat ttttgttcct attccaattt cactacttac ataaatttta cctttatgaa
     5101 tctctattat ttctttagaa atagccatac caagacctga acctttatgc atctcacctg
     5161 tattagtacc tctataatat tttttaaaaa tatgtttcaa atcacttttg cttattccaa
     5221 ttccattatc ttttattata atttctatat tatctttttt tacaatttct acacttatca
     5281 aagttccttc gctattatga actattgaat taaatataag attggttata gcccttctga
     5341 ataagattga atctacatat acttcaatta aatcttcatt gctttcaaat tcaatattcc
     5401 tatttttata tctattatca tttaaaatat caattattat atttctgact aaactgacta
     5461 aatttatttt tttcttgtcc agaactatag tattattttt taatcgagta gataaattta
     5521 aatcatcaac taaatctttt atatacttag acttattata tataatttct gtatactctt
     5581 gtatttcatc ttcttcaaac tcataatctt tgtcattaat tatctctgca tacccttgta
     5641 tagatgctag aggtgtcttt atatcatgtg ttatatttgc cagccattcc tctctcaact
     5701 cttcattttc ttttctctca acttcattta ctcttaaagt atcagctaac atatttatat
     5761 ttttaaaaac ttcttcatat atacctctgt ccttaagata taaactataa tttccattag
     5821 ccatagtctc aatactccat agtacgcttg aaattggttt tgtaagtttt ctactaaata
     5881 aatatcctac gcctaaagct accacgctat caattactag tgttataatg ataactttat
     5941 ttaaagtttt tttaatgtta ttttgagaat aggttaaaat tattcttgat atatctctac
     6001 taggtatacc tactattatg tttaaatgct ttttgtttaa atatttttct agtacaaaat
     6061 taacatactt tgtttctata tacttgtaat tattcaccat ttgaaagggt gtatattttt
     6121 tgggagtatc ctttggcaca tttactccat aaacttcctc tcctaaatca tttaaaacct
     6181 gtatccagga atttgatttt tctaattttt cagctccaat cttagatagt ttaaattctc
     6241 catctttttc atataaatct ttttcaaaag aacgtgcaaa ttcttcagga ttatttccag
     6301 aatctacaac tttaaaaaag ctattagttg aaatcacata caatatagat atgatattta
     6361 taattactac tgatatagct acaaacacaa ttgtaaatat aaaatttcta gtaatcttcc
     6421 acttcatatt ctaatcttcc tttactgcta atttatatcc aagacctttt attgtcagaa
     6481 tatattcagg ttttgaagga ttatcctcta atttttccct aatttttctt atatgaacca
     6541 ttatagtatt gtcaaatcca acataatctt ctccccacac tttatcccat agtttttctt
     6601 tacttataat ttgattttga ttatgtgcaa ataaagatag gatttttagt tcctttgcag
     6661 acagtcctat agattttcca tcttttataa gttctgccct actttcattt aatatatatg
     6721 ggccaaatga tataattttt tcttcattat tatttagatt tttactttcg ttttgagcat
     6781 cactaaaata acttaatctt ctcaaatgcg ctttaactct aaaagccact tccttagggc
     6841 taaaaggttt tgtaacataa tcatctcctc caatcgctag tccaagtaac ttatctaatt
     6901 cttcggtttt agctgataaa aatattattg gagaattgga ggtttttctt atttctttac
     6961 aaatatcata accttcgcca tctggtaaca taatatccaa tacaatcaaa tcaggattga
     7021 tacttttaaa taattctaat ccttcttttt tagtttttgc agtatatata ttattaagac
     7081 cctctttttt taggacagtt tctaaaagct tcaatatttc tacttcatca tctattatta
     7141 aaacacgatt ttccataaac aacctcctgt aataatttac atatatatta gcatatattt
     7201 tatctaaaat tcatttatct tatttttaat taaactaaaa tataaataaa attactatta
     7261 tttttagtta aaaccttttg cttgatgtaa aattttcata tgttatttta gttaacacta
     7321 tcattaatat aattgagatt actatagaaa aaactatcaa aatacttggt acattatact
     7381 taaccatttg ttggctcatc ataatccaat ttggagtcac tgacataaat tttatagctt
     7441 tatctggtat aaaacctaca ctaaagtatg gaattgccat tatcaatcca cctagtataa
     7501 atggaattac agccgattta aatatagatg aaagtgttaa gcctatttgt gcaaatataa
     7561 aagaagcaaa aatagtagtc aatataacaa ttatatacat ttgtattact gtaagattta
     7621 aattactatt tccaaagtac cataagttat gaatcgattc atttaaattt ttaaaatcaa
     7681 aaaaactaaa acctctaatt cctactaaca atataaaaga aatattaata atagataaaa
     7741 ttaatccatt agctactagt tttgataata ctactgattt ttttcccttt ttagttgtat
     7801 ttattaaacc tgtcatatta gaatcatatt ctttaatgta atttccagaa gttattataa
     7861 acattatcaa acctattaga ggtattgagc cttctatctt gtaaaatata tctgcataag
     7921 tatctaaaac atcaaacttt ctaatcagaa aaaatccaaa aactattaaa aacagccaaa
     7981 ttatccaaac actctttgat tttaatattt ttatcatttc agctttaact aaatacatca
     8041 tttctactac acctcccttg catcttctaa atcaaaataa aacatgtata catcttcgaa
     8101 ttttgcttga actggttgta tattatcctc gcttggaata tcctctgaaa caattctaag
     8161 tactattgaa tcaatatcac tttgaatact tacaacctta tatttagatt gtatatcatc
     8221 aacttcacat tcatttttaa cttgaacatt atatacttta ttttccatgt gacttaatat
     8281 gtctttgtgt gttcctttca ttaggagttt gccatcttta atcattattg tttcctttgc
     8341 aactgattct atatcggaaa ttatatgagt agaaagtatt attatcttat ctctagacat
     8401 ttttgctatt aaattcctaa atctagctct ttcttgtggg tcaagacctg ctgttggctc
     8461 atcaaggact attatcttag ggtcgtttag taaaacttgt gctatcccaa ctctcctctt
     8521 catacctcct gaaaattttc ctatagactt atttctcaca tcataaagac ctactaaatc
     8581 taataattct cctatcttac tatttaaata ttttcccctt aaacctttta aagctccaat
     8641 atactgtaaa aattcttttg ctgtaaaatt tttataaact ccaaattctt gtggcaaata
     8701 tcctaaaata tttctataat cagcatccat tttaaatata tcctcaccat tataaagtat
     8761 ctgaccttca tcaggtttta tcaacgttgt tatttgtttc attaatgtag attttcctgc
     8821 tccatttggt cctagaagac cataaactcc attttctaga gttactgtaa tcttgtcgtt
     8881 tgctactttt ttaccatata tctttgaaag attttctatt tctaatttat tcatcttaac
     8941 tcccctttta tctacttata taatctgatt ttattactaa aaaacataat gacatagcac
     9001 ctatcaatat tgccatcaaa agtcctattg ctatattgac aatgtctata ttcactttaa
     9061 atatcataat atcacctaga taatttccta ttattttatt aaaaccaaat atcaaatcat
     9121 aataatttac atttgataca actgaaagta attttttagg taatatatgg attaccgaca
     9181 ttatcttagt tatagatata ataaatataa aaaaacttat tgaagatatt gaagacttac
     9241 ttataaaaga cccaaatatg gctatcatgg cccatccttc aaacatcaaa agctctatgc
     9301 ctatatttaa tattacatac tttgttatac ttaaattagt tttcaatgca actacattgt
     9361 ctactattct aaaagattct aagtctccat taacaggtac tccttgttgc atacaagtta
     9421 taataaatat tcccattaaa taaacagaat aaattactaa tggaactaat aaagcaacaa
     9481 atattttaga ctttaatgct tttttcttct cttttgatga tagtatgata tcttttaccg
     9541 aagtaacgac ttcatctacg taaatattgg atataattat agatataatg attaacattc
     9601 ctacatttat aagtgaattt gtaactctat agttaaatac tttccaaaaa
//
LOCUS       DAGSHT010000125         9623 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.129, whole genome shotgun sequence.
ACCESSION   DAGSHT010000125 DAGSHT010000000
VERSION     DAGSHT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.129"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1002
                     /locus_tag="MJB72_002611"
     CDS             <1..1002
                     /locus_tag="MJB72_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY8263695.1"
                     /translation="DKGYYFNSNWKDIHIGIYDTKLGDSLKYITNEYIENMERVKYDY
                     RELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKALEYVKEQTEYLC
                     LEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQTRRICLEAIKKDGR
                     CLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMALQYVNKQTDKICI
                     EAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTEDICIEIVRQNGK
                     MLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKEYVKIAIDDCI"
     gene            1141..1593
                     /gene="tsaE"
                     /locus_tag="MJB72_002612"
     CDS             1141..1593
                     /gene="tsaE"
                     /locus_tag="MJB72_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBY8263696.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1593..2309
                     /gene="tsaB"
                     /locus_tag="MJB72_002613"
     CDS             1593..2309
                     /gene="tsaB"
                     /locus_tag="MJB72_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBY8263697.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2299..2775
                     /gene="rimI"
                     /locus_tag="MJB72_002614"
     CDS             2299..2775
                     /gene="rimI"
                     /locus_tag="MJB72_002614"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBY8263698.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2781..3797
                     /gene="tsaD"
                     /locus_tag="MJB72_002615"
     CDS             2781..3797
                     /gene="tsaD"
                     /locus_tag="MJB72_002615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBY8263699.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4094..6802
                     /gene="hpdB"
                     /locus_tag="MJB72_002616"
     CDS             4094..6802
                     /gene="hpdB"
                     /locus_tag="MJB72_002616"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBY8263700.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVQILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6806..7063
                     /gene="hpdC"
                     /locus_tag="MJB72_002617"
     CDS             6806..7063
                     /gene="hpdC"
                     /locus_tag="MJB72_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBY8263701.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7056..8006
                     /gene="hpdA"
                     /locus_tag="MJB72_002618"
     CDS             7056..8006
                     /gene="hpdA"
                     /locus_tag="MJB72_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBY8263702.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8321..9283
                     /locus_tag="MJB72_002619"
     CDS             8321..9283
                     /locus_tag="MJB72_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY8263703.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFRLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9382..>9623)
                     /locus_tag="MJB72_002620"
     CDS             complement(9382..>9623)
                     /locus_tag="MJB72_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY8263704.1"
                     /translation="IILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNS
                     KESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         3761 a         1001 c         1814 g         3047 t
ORIGIN      
        1 gataaagggt attattttaa tagtaattgg aaagatattc atattggtat ttatgataca
       61 aaattaggag attctttaaa gtatatcact aatgaatata ttgaaaatat ggaaagggta
      121 aaatacgatt atcgtgaatt aaaaaatata gaaaatcaaa ctgatggtat gtgtatttta
      181 gcaataaggc agaattacaa ggcattagag tatgtaaaaa atcaaaccga agaattatgt
      241 ttagaagcaa taaagtataa ttataaagca cttgaatatg taaaagaaca aacagaatac
      301 ttgtgtcttg aagctataaa aaaagattgt aatgcactta agtatgtacg aaataaaaca
      361 gaagggctta ttataaaagc aataagtcat tcttccaata tagatgttgt aagtatacta
      421 aaggctttag agactcaaac aagaaggata tgtttagaag caattaaaaa agatggcagg
      481 tgtcttgcat acgttagaga acagagtgaa gaattatgta tagaggccat taagcaaaat
      541 tataaagcat taaaatatgt aaaaaatcaa acggaaaaaa tgtgtataga atccgtaaga
      601 caaaatggta tggcattaca atatgtaaat aaacaaactg ataaaatatg tatagaagct
      661 gtcaaacaag atggaagaag tctacaattt gtcaacaata agacagaaga aatatgtata
      721 aatgctataa gatatttaaa taaaaaatat aatattaaag atgtactaag ttacatagat
      781 aagtatacag aagatatatg tatagagatt gtaagacaaa atggaaaaat gcttatgtat
      841 ataaaaaatc aaacagaaaa aatgtgtatt gaagcagtaa aagagaacta taagtctctt
      901 aagtatgtaa aagagcaatc tgagagaata tgtaaagaag cattgaagca aaaccataaa
      961 gcaaaagagt atgtaaaaat tgctattgat gattgtatat aaggtgtaga tacagccatc
     1021 aatagtgaaa gataaaaaaa gataaagaca agtactttat aataatctag gcttatacta
     1081 taattaatta agcagtgata taaattcaca tgacgattat atcaaaatgg agatgaattg
     1141 atggcaaaaa tatatttaga aaatgaaaat aaaactagag aaataggata taaattaggt
     1201 aaacttttaa aagaaggcag tgttatctgt ttagtaggag atttaggagc tggtaaaact
     1261 acaatgacac aaagcttggc agattcatta gggatagaag attatataac aagtcctact
     1321 tttactataa taaatgaata tgaaggaaaa atacctttgt atcattttga tgtttataga
     1381 ataggtagta gtgatgaaat gtatgatatt ggttatgatg aatatgtaaa ttcaaatgga
     1441 atctgtataa tagaatgggc taatctaata gaagatatac ttccaaaaga atatttgaat
     1501 atagaattga gatataaaga tgaaggaaga gaaatgatat taactccaaa aggagagttt
     1561 tataaagaga tagttgagga gttgattaaa taatgaaaat actaggtatg gatacgtctt
     1621 ctatggcagc aagtgtagca gttgttgaag atgataactt aatatgtgag tttacagtaa
     1681 acaacaaaaa aactcattca caaaagctta tgccaatgat agaaaatatg ttgagtatga
     1741 gtgatttaag tataaaagat atggatttat tagctgtatg tataggtcca ggttcgttta
     1801 caggacttag aataggtatg gctacagtaa aagctatggc ccatgtaaat aatataccaa
     1861 taatagcagt aaattctttg gaatctctag caaataacat aaatttctgt gatagaaaga
     1921 tatgttgtat attagatgcg cagaaaaatc aagtttattc agctaaatac aagtttgaaa
     1981 atggagagat gatagaatta gatggtgttg atgttgtaga gtttgatgct ttagtagatg
     2041 aaattgtatc aactaatgaa gaatttataa tagttggaga agctgtatac aaatataaag
     2101 acaagttaga gaatataaaa aatataaaaa taccttcacc agctaataat gtatctaacg
     2161 caggaagctt atgctcttta gcattaaata aatataataa aaatatagat gtacatactt
     2221 gttatacgat aaatcctatg tatatcagaa aatctcaagc tgaagtacaa tatgatgaaa
     2281 aaatgaagag gctaaataat ggaagataat acaaaactaa tagtagatga tataaaaata
     2341 gaagaaatga ctacagaaga tatagatgaa gtttttgaag tagaaaaaaa ttgttttgaa
     2401 gattattggt caaaagagtc ttttagaaaa gagttgtcta atgaagtagc aaggtatata
     2461 gtagctaagt taaatggaaa aattgtcgga tatgtgggaa tttggcttat acttgatgaa
     2521 gggcatataa ataatgtggc tgttcatagt gactatagag ggaaaaaaat aggggacaag
     2581 ttgataaaag gcatagttga tttatgtaaa gataataata ttgcatctat gacattagaa
     2641 gttagagcat caaataaaat agcacaaaat ttatatagaa aatatggatt taaaatgggt
     2701 ggaataagaa aagaatacta cagtgataat aaagaagatg caattataat gtggaaccaa
     2761 ttaaaagagg tgtagagttt atgagtgata taataacatt agctatagag agtagttgtg
     2821 acgaaacagc tgcatcagtt ttaaaaaatg gtagagaagt actttcaaac ataatctcta
     2881 ctcaaatcga gacacataaa aaatttggtg gtgtagttcc agaagtggcc tcaagaaaac
     2941 atgtggaaaa tattgatata gttgttcaag aagctttaga caaagcaaat ataggtttta
     3001 atgatataga ccatatcgca gttacatatg gacctgggct agtaggtgct ttattggttg
     3061 gtctatctta tgctaaagca cttgcatata ctttgaatat tccacttgta ggagtaaatc
     3121 atatagaagg gcatctaagt gcaaactata tagagcataa ggatttaaaa cctcctttta
     3181 taacattaat agtatctgga ggtcatactc acttggttga agtaaaagat tatggcaagt
     3241 atgaaatttt aggaaaaaca agagatgatg catctggaga agcatttgat aagatttcaa
     3301 gagctatgaa tttaggctat ccaggaggtc ctattattga taatctggcc aagaatggaa
     3361 ataaacatgc tatagaattt ccaagagcct atttagaaga agatagctat gattttagtt
     3421 ttagtggatt aaaatcctct gtattaaatt atctaaatgg aaaaagaatg aaaaatgaag
     3481 aaatagtagt tgaagatgta gcagcatctt tccaagaggc tgtagtagag gttttatcta
     3541 ctaaagcact aaaagcagta aaagacaaag gatacaatat cataactctt tctggtgggg
     3601 ttgcttctaa ttcggggctt agagctaaaa taacagagct tgctaaagac aatggtatta
     3661 ctgttaagta tcctcctcta attttatgca cagataatgc agctatgata gggtgtgcag
     3721 gatattataa ttttataaat gggaaaacgc atgatatgag tttaaatgca gttccaaatt
     3781 taaagataaa tcagtagagt tagatgaatt tgtaaatata aaagtattgt ttcaaaaaag
     3841 taagtagaag tgaaattaaa tataataaaa ataaatatag tatatattaa aacttgaagt
     3901 aaaactattt ataatcttta tattccaatg aattgcaaca aattttagga tatacagatt
     3961 gtctttggct tagatttgct tcaagtttaa ttttttctat agaaagatta ttttaaaaag
     4021 tgggtaatat accctttttt ttcattctta aaaagtttta tactaattat agaaagatta
     4081 aggaggggaa gaaatgagtc aaagtaaaga agacaaaata agaagtattt tggaagcaaa
     4141 aaatattaaa tcaaattttc aaaataaaga aaatttaagt gaatttaatg aaaaaaaagc
     4201 aagtaaaaga gcagaagact tattagatgt atattataat acattatcta ctgctgatat
     4261 ggaatttcca tattggtata atagagaata tagaaaatca gatggtgata taccagtagt
     4321 tagaagagct aaagctctta aagctgcttt ttcacatatg acaccaaata taatacctgg
     4381 tgagaaaata gtaatgcaaa agacacgcca ttatagaggt tcttttccaa tgccatgggt
     4441 aagtgaaagt ttctttgttg ctcaaggaga acaaatgaga gaagaggcaa agaaattagc
     4501 cagtaacaca gctgatgaac ttacaaaatt tgggtctggt ggaggaaatg ttactgaaag
     4561 ttttggaaat gtcgtttcaa tagctggtaa attcggaatg agaaaagaag aagtacctgt
     4621 acttgtaaaa atggcaaagg aatgggttgg aaagtctgtt gaagatttag gttttcacta
     4681 tgaaaaaatg atgccagatt atgatttaaa agaaaaccta atgagtacac ttatatgcat
     4741 gtttgattca gggtatacac ttcctcaagg tagagaagtt ataaattatt tttatccttt
     4801 aaattatgga ttagatggaa ttatagaaat ggcaaaagaa tgtaaaaagg cagttgcagg
     4861 aaatgcatct ggagatgggc ttattggtat ggaccgtcta tatttttatg aggctgttat
     4921 tcaagttatt gaaggattac aaacttggat tttaaattat gcaaaacatg caaaatattt
     4981 agaaagtata gagacagatt tagaagctaa aaaagaatac tcagatttag tgcagatatt
     5041 agaacatatt gcacataaac aacctcgtac atttagggaa gctcttcagt tgacttatac
     5101 aattcatata gcatcagtaa atgaggatgc tatatctggt atgtctatag gacgttttgg
     5161 tcaaatttta tatccttggt atgaacaaga tatagaaaaa ggactaatta caaaagaaga
     5221 ggttatcgaa ttattagagc tttatagaat aaaaatcact tgtattgact gttttgcttc
     5281 agcgggcgta aatggaggag tactatcagg caatacattt aatactttat ctataggtgg
     5341 attaaaagaa gatggttcta caggagctaa tgaactagaa gagttattat tagaagcaag
     5401 tatgagatgt agaactcctc aacctagttt aactatgcta tatgatgaaa agcttccaga
     5461 agatttctta atgaaagctg ctgaatgtac aaagcttggt tctggttatc cagcatgggt
     5521 aaacaactca aatggaacaa catttatgat gaaacaattt gctgatgaag gtatgactgt
     5581 tgaagaagca agagcatttg cattaggtgg atgtttggaa acgtctccag ggtgttggaa
     5641 acagttaact ttaaacggaa aaacatactc tattgcaggt ggagcaggtc aatctgctgg
     5701 ctctggagtt cactttatag ctaatccaaa aattttagaa cttgttctta tgaatggtaa
     5761 agactatcgt atgaatatac aagtatttga gcctcataat aagccattag atacttatga
     5821 agaagtcata gaagtattta aagattatta taaacaagca ataaatgttt tagaaagagc
     5881 aaataacatt gagttagata tatggagaaa atttgatact tctataataa attcactact
     5941 aaagccagac tgtttagaca aaggtcaaca tataggaaat atgggatatc gttataatgc
     6001 aacattaaac gtagaaactt gtggtactgt tactatggtc aattcatttg cagctcttaa
     6061 gaaacttgta tatgatgaca aagcatttac aatagaggaa atgaaggatg ccatattaaa
     6121 taattttgga tttaaagatg cattagaagt aggaaattat tctatggcgg accaagtaaa
     6181 agtagataag actggtaaat atgatgctat ttataaggcc tgtcttgatg cacctaaata
     6241 tggaaataat gatttgtatg cagataatat tcttaaaaac tatgaggtat ggttatcaaa
     6301 agtatgtgaa gaagcacaat ctttatatgc taagaagatg tacccttgcc aaatatctgt
     6361 ttctacacat gggccacagg gagctgctac cttggctact ccagatggta gactaagtgg
     6421 aacaacatat tcagatggtt cagtctctgc atatgcagga acagataaaa atggagtata
     6481 tgcattattt gaatctgcaa ctatttggga ccaagcagta gttcaaaatt ctcagatgaa
     6541 tttaaaactt catccaacta ctataaaagg acaacaagga actaaaaaat tattagattt
     6601 aacaagaagt tatttaagaa aaggtgggtt ccatattcaa tataatgttg tagattcaga
     6661 aacattaaaa gatgcacaaa aaaatcctga caattatcgt caattaatgg tacgtgttgc
     6721 tgggtttact caatattggt gtgaattagg taaaccaata caagatgaag taattgctag
     6781 aacagagtat gaaggggtgt aaaatatgag aaagcatagt gattgtatga atttttgtgc
     6841 agtagatgca accaaaggaa tttgtagatt atcaaaacaa atgattaatt tagatgatgc
     6901 agcatgtcca gagataaaag taatgccaaa atgtaaaaat tgtaaaaatt ttgttgaagc
     6961 taatgatgaa ggtataggca aatgtgttgg cctagagaaa gaagactggg tatattcaac
     7021 attgaatgca attacttgtg aaggacatgt gtttaatgag tagtcaaaag caattagaag
     7081 gcatgatttt tgatgtacaa agcttttcag ttcatgatgg tccaggttgt agaacaactg
     7141 tatttttaaa tggatgtcca ctgagttgta aatggtgtgc aaatccagaa agttggactg
     7201 taagaccaca tatgatgttt agtgaattat cttgtcaata tgaaaatgga tgtactgtat
     7261 gtcatggtaa gtgtaaaaat ggtgcattga gctttaatct tgataataag ccagttatag
     7321 attggaacat ctgtaaggac tgtgaaagtt ttgaatgcgt caactcatgt tattataatg
     7381 cgtttaaatt atgtgcaaaa ccttacactg tagatgaact tgtgcaggta attaaacgtg
     7441 attctaacaa ttggagaagt aatggaggag taacatttag tggaggtgag ccactacttc
     7501 agcatgaatt tttacatgaa gtattattaa agtgtcatga ggtaaatata cacactgcca
     7561 ttgaaacgag tgcatgtgta tcaaatgaag tttttaataa aatatttaac gatattgatt
     7621 ttgcatttat tgatataaag catatggata gagaaaaaca caaagaacaa acaggtgtgt
     7681 ataatgattt aatattagaa aatatatcaa accttgcaaa ttctgattgg aatggaagat
     7741 tagtacttag agtaccagtt atctctgggt ttaatgatag tgatgaaaat ataagtgata
     7801 ttatatcttt tatgcacaaa aataatttgg tagaaattaa tctattacca tttcacagat
     7861 taggagaatc aaaatggatt caattaggca aggaatatga gtattctgat aagggtgatg
     7921 ttgatgaagg tcatctagaa gaattacaag atatattttt ggataatggt atagcttgtt
     7981 atgttggcca tgagacagct ttctaaaatt aaatacaagt tttaattaaa aaaggatgtt
     8041 acactaaaag ctagagttac atcctttttt atgtggtttt tatgtagaaa taattaagta
     8101 aagttttata tgttttatat attgtttata ttgctttatg tccataaata ttgctttaaa
     8161 tatactttat atatttttta tagtttaaat tacagttaga gaatatatta cgtttacttc
     8221 attgaagcag aagttttgtg gtaaaatact actatgaaaa aatttaatag aatatatttt
     8281 agaaacaact aaaaataata gttaggagga agacatctat atgaacaaat catggagcaa
     8341 cttgattatt aagaataaag acagaaaagt acgttcaggg tggattatta tagctgttat
     8401 ggctaccttt tacatacttc agtattattt aagcatgata cttgtggaaa taataagaaa
     8461 aatcctggtt gctacaggtg acattaattt aaaaacaaac tatttttcag aatatgctaa
     8521 ttggttaaat gatgttgggt tacagattgt atttcaaatt ctaacagatt cacttatgat
     8581 tattattcct ataattgtat ggagatttat tatgaaaaat cctattagtg aaatgggatt
     8641 acattcttat agaattaaga aaaaagaagg atatgtagga atgttattag gaatatttaa
     8701 ttgtacagta atctttatta taacaataac aattggtggt ggtcatgtta cttcatgggt
     8761 accaaaaatt tcacctttaa cagtttcttg gatactggtc tttattttag ttgcttttgc
     8821 agaagaaatt ttaaatcgtg gtttttttat ggcagtttta cgaagatgta aaaatattta
     8881 ttttataatg attgttccat ctgttatttt taggttgatt catatatgga atcctgatgt
     8941 tacttttttg tctgtaatta atattataat tattgggatt cttttttcat atatgtttat
     9001 aaaatcgtcg aatatatgga tgtgtatagg ttatcatttt gcatggaatg tatttcaggg
     9061 gattatatat ggtatgcctg taagtggact tcaagttcca gggattataa ctacacaaat
     9121 cactcatggt aatctattaa atggaggaat gtttggtatt gaaggtggaa ttttgactac
     9181 ttttgttaat ctacttagct ttatatttgt atggtattac tatagagatt ctaagtatga
     9241 ttttgttagt gataaagtaa atgtgaccaa tatcaagaat tagttgatta taataaattt
     9301 taataatatc ttctaagctt aaagtattct gtaggattgt tggaaatttg caaaaatata
     9361 aaaataactt ggatattcta tttactccaa gttattttta tattcttttg taaatagaaa
     9421 tggaacataa aaaattaaaa caaccataga tactattagt atatcatggt aagtgatagt
     9481 tgattcttta gaatttgtga taagaaaagc actattattt aaaacatgaa ttatagcaca
     9541 aaaccagata ttcttagttt ttatataaag atatcctaaa aatattgaaa ttccaactat
     9601 atgtattgaa cgtaatatta ttc
//
LOCUS       DAGSHT010000126         9610 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGSHT010000126 DAGSHT010000000
VERSION     DAGSHT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.130"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            677..2764
                     /locus_tag="MJB72_002621"
     CDS             677..2764
                     /locus_tag="MJB72_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8263705.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2779..3066
                     /locus_tag="MJB72_002622"
     CDS             2779..3066
                     /locus_tag="MJB72_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8263706.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            3093..4364
                     /locus_tag="MJB72_002623"
     CDS             3093..4364
                     /locus_tag="MJB72_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBY8263707.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4422..5021
                     /locus_tag="MJB72_002624"
     CDS             4422..5021
                     /locus_tag="MJB72_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY8263708.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            5026..5931
                     /gene="dapA"
                     /locus_tag="MJB72_002625"
     CDS             5026..5931
                     /gene="dapA"
                     /locus_tag="MJB72_002625"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY8263709.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            6037..6669
                     /locus_tag="MJB72_002626"
     CDS             6037..6669
                     /locus_tag="MJB72_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBY8263710.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVKKI"
     gene            7147..>9610
                     /locus_tag="MJB72_002627"
     CDS             7147..>9610
                     /locus_tag="MJB72_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8263711.1"
                     /translation="MKEDIGLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDTYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNIVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISI"
BASE COUNT         3654 a         1080 c         1723 g         3153 t
ORIGIN      
        1 tgccttatat aaaagatttt ctaaaaacac aacacaaata ctttaataat ataagtgaga
       61 atcatatcac aaatgagaga aataattctc cttattaatt aattttaatt aataaatatt
      121 acaatagatt tatctggaat ggaaggtatg cagagtgtat ttcattagat gaaacactta
      181 aaagaatgaa atagataagt atcaacttat tatatgagag aaactttaac tttcttggtt
      241 taaaatatta aaattataag gaacatttat tattaaatac tttatttata tgaattaatt
      301 gaagaaaaag gatattaaac tacaagttaa aaaataaatt taatatatta gaattataaa
      361 taaggcacat taattttatt ttaaattaat gtgcctttta tttatggtaa tttatgcata
      421 attagtatcg ttttgattct ttaaataaat ttttcttggt ttcttcacat aaggaacttt
      481 ttttaattta taaaatctac agttatatta tagtatctat tttagagaag tttaattttt
      541 gtatcaatct aaaacaaaag ttatacttaa atgaaattga aaacgataaa attctcaaat
      601 aaaataagta ttgtggaggt gatgaaagaa tccaatgata atatgaagac taatcttaat
      661 aagaaagtag gggttaatgg tagtggacaa agaaattgta tcaattctcc agattttatt
      721 aaatagcaca ttgattacaa ctaatggatt acaagaggag tctaattcat caaagagaca
      781 aattacttat agaataaata aaatcaatga catgcttaaa gtaaaaaatg taccacttat
      841 atgtttaaga gctgataaag atattatcgt tagaaaagaa acaaaaaaag caattaaaga
      901 aattttagaa aaaaactatt ctaagaatac ttattatttt agtaagacag aaagactttt
      961 atatatgtat ttaatgcttt ttattaacat ggaggatatt tcaattaatc attttataaa
     1021 ttctataaaa gtaagtaggt ctactgtaaa tcttgatttc aaggatttga taccaaagct
     1081 tgaaaagaag aacattaaag ttaagaacaa tcgtataaat ggatattaca tagttggcaa
     1141 tgagatggaa attaggagag tgcttattaa aaatattata gaaactttgt ctaatgagaa
     1201 aagttcaagg gtatttgata tttttattaa agaatataat ttagatagtt ttgaagaggc
     1261 taaaaaaatt atattaaagc taataaaaaa atatgaaatt acttttgttg aagatagatt
     1321 aatagaattt atatatatat ttatctttct aaaggcaagg atgtattcaa ctagaatagt
     1381 tactcaggaa aatgtagata ttcctaatat ttcagttatg agttcaatga aggagtataa
     1441 atttgctaag gaattattag aagtatatgg gagtgaagat aaaataaagc ctcacaatat
     1501 aatgtatatt agtgcttgga ttttaggtat atcagtagga aattatgaag aagatacaga
     1561 agataaggat gtaatttcaa aaattgtaaa taaaatgatg acaaagtttg gatatttaag
     1621 tggagtttac tatataagcc aagaaaaaat atttaaacaa ttatattctc attttagacc
     1681 tgcctactat agacttttat ttaaattacc tatctataat ccgatttgtg aaaaagtaaa
     1741 agaagaatat aaattagttt atagaattgt aagtaatgca atgaaagagt tttgcggatt
     1801 atttggagaa gaaattcagg aagaagagtt agcatatctt acaatgcact ttgcaacact
     1861 tttttcaaat aaaaaagaat ttgatggttt tagaaaaaga atagcattaa tagtttgttc
     1921 aaatggcgtt ggtagttcag ctattttaca tgcagaactt acaaacttat ttcctgactt
     1981 acactttata ccaatgtcgg aatcagttgg attagaaaat ataagtgaac ctgtagatat
     2041 tgtatttact actaattata acgcagaggg tttaaaaaca gatgtaccag taattaaagt
     2101 aagccctgtt atgaccacaa aagaaaaata taagattaca cgtgaagtgt atattcaatt
     2161 aggtgatatt tttttaagac aacctaaaat agatgaggtt atggatataa taaaaaaata
     2221 tgcaaatgta acctctgaaa gtattctttc aagtgagcta ttagcttatt ttacacagat
     2281 agagaatttt acatctaagg aaggagaggg gcctatgcta agtgagatta ctaatgaaac
     2341 actagtaaaa ataaaggtaa aagctaagaa ttgggaggaa gctattagac agtcagcttc
     2401 tgtattagtt gaaaataaca aagtaactga agggtatgta gatgctatga ttaactcagc
     2461 aaaagcatct ggaccataca tagtaattac aaaacatgtt gcacttccac atgctagacc
     2521 agaagctggg tcaaaagaga ttgcaatagg cattgctact ttagatagtc ctattgagtt
     2581 tgggaataac gaaaacgacc ctgtaaaata tgtattttgt ctaagtgcaa tagacaataa
     2641 tagccattta cgtgctatgt ctgagctagt tgaactattg gaagaagatg agttttttaa
     2701 ggttttagat agtgctaaag aagctaaaga aattattgat tttataaaaa cacacgaatt
     2761 ataaaggagg atttaattat gtataaaatt ttaatcgcat gtagagcagg agttggctca
     2821 agtttaatgt taaaaataaa gactcaacaa attattaagg aaaataactt tccaattgaa
     2881 gtagaacatg gttcattaga ttcactaaat ggattcaatg gagatgctgt agaaacatta
     2941 attgatgttg ctgaagaatt aaaaaataaa aatttgaagt ttgatataat tgggattcac
     3001 aatattgttg accgtaatga aataaagaca gcactagaaa aattccttgc ttcaaaagag
     3061 ggataaattt aagatgataa ggaggaatat ttatggagtt tattattagt ttgctgagta
     3121 cacctgctgt tttgcttggc gtagttgcat gtataggatt gattcttcaa aagaaatcag
     3181 ctgttgaagt atttacaggc tcttcaaaaa cacttatcgg ttttttgata tttggtattg
     3241 gtgcctctgc tatgacagga gcacttcaaa atttcaataa actgttccaa catggatttg
     3301 gaattactgg tgttatagct tcaccagaag cagctacagc acttgcacaa ggtacatatg
     3361 gttttgcagt ttcatgtaca ttaattcttg gattcatatt aaatttagta tttgcacgta
     3421 ttacaaagtt taagaatatt ttctttacaa caggtcatag tttgttcttt tcttgtgtat
     3481 tagttttaat tatgaaagca catggatttg ataatacaat cactatttta ataggaggaa
     3541 ctatcttagg gtttatgtct gctgcactac ctcaattctg tcaaccattt atgcgtgaat
     3601 taacaggcgg cgacgaacaa gctattggtc actttaacat gattggttat ggtttatcag
     3661 gttatattgg gcgtttattt tcaaaacata aagatgatac aacagagact atagaatttc
     3721 ctaagtggtt atcaatattc agagacttct taatgggtct atccatagtt atgttaatct
     3781 tattttatat tgcaacatta aaagctggtc aagcatttac tcaagaaatt gctggttcaa
     3841 cacattggtt ggtttttccg cttatagagg catttacatt tgtggcaggt atgtcaatat
     3901 tgatgtcagg tgttcgtatg ttcttagctg aaataactgc agcatttgta atcatttcag
     3961 aaaagtatat tccaggttca cgtcctgcgc tagatgttcc aaccgtattt ccatatgcac
     4021 caaatgcagt tattattggg tttatatcag catatgctgc tggtctatta gcaattgcta
     4081 ttatggcatc atttcctagt atatttcctg tagtaattat tccagctgct cacatatgtt
     4141 ttttctcagg aggtacagct gcaatatttg gcaatacttc aggaggatgg agaggggcta
     4201 ttgctgggtc atttgttgtg gggttattac ttgcattttt gccagttata ctatatccag
     4261 tttatgggac tcttggtata gaaggagcaa ctttccctaa tattgattac aacgtagttg
     4321 gttcgatatt acataatatt ttacaattta taaaacctat ttaaactaaa ttatataaaa
     4381 gtatagtcat agttactatg atttcaagga ggatacatat tatggaaaat acattatctg
     4441 aatttttaga taatatatct aatgaattgt cagaatttat tgcctcaata gatgagacat
     4501 ctataaataa agcatgcgaa ttaattcttg aagctgaaaa aaatcacgga cgtgtacatg
     4561 tgactggtat tggcaaacca ggacatgtat cgggatatat ttcatcattg ctttcatcta
     4621 ctggaacgtc agcatatata ttgcatggta cagaagcagt acatgggtca agtggtcaag
     4681 tagttgaagg agatgttgtt attgcaatat caaatagtgg tgaaactcaa gagttaaaag
     4741 caactcttaa gactctaaaa gtaaatggag caaagataat tggtgtaagt ggtaatgaga
     4801 gttcattttt aaaaaatatt tctgatatat tcctgtttgc aggtgttaaa caggaaggag
     4861 attgtcttaa taaagctcct cgtgcttcaa ttttagctga aacaataata ttacaatcat
     4921 taagtgttgt attacaatat gctaaaggtc ttaatacaca gcagtatttg aagtggcatc
     4981 cagcaggaag tcttggaaag tctattagag agggaatata attttatgaa atataaaggg
     5041 attatatgtg caatgataac accatttgat gaaaatcaaa atattaattc acaagcaaca
     5101 tgtcaattaa ttgattattt gattgaaaag ggaatttatg gattatttat tttgggcact
     5161 aatggtgaat gtcatgtatt aacagatgat gaaaaagttg aatttgcaaa gattgttatt
     5221 aatcatacaa ataatcgtgt acctgttttt gttggaacag ggggtaattc aactcgtgaa
     5281 gttgtagatt tatctaagag aatggaagcg attggagcaa gtgcattatc tattattaca
     5341 ccatactttg ttacaccaac acaacaggaa ttaatcttac attacaaaac tattgctaat
     5401 tcagttaatt tacctattat aatgtataat atgccaggta agactggaat taatattgaa
     5461 ccagagtcag tacgtgaatt atcaaagata aagaatattg ttggaattaa ggatagtagt
     5521 ggtaaacttg acaacatgaa agcttatatt gaagcaacga agaatgagaa ttttagtgtg
     5581 ttttcaggct cagattcact tattctggat agtctaaaag ctggtagtca aggagctgtt
     5641 gcagcaacaa caaacttcct cacagagatt ggtatctcta tctatgaaaa ctttatgaaa
     5701 ggggatttgg aagaagctca aaaagctcaa agtagtatag aagaacttcg acgtatactg
     5761 aaacttggaa caattccatc tgttatgaag aaaactattg tcttgaatgg aattaatgtt
     5821 ggaactgcac gacttcctgt aactgaacca actggggaag tacttgaaga aattaaacga
     5881 gtagttgaaa attataggac aatacttaat aaaagtagtg agaatagata atataaatag
     5941 ggaaattaca ctggtatata attaatatat atattataaa acatgactaa aattaatgaa
     6001 taagtaaagt ggtttgatga gtatagaggt gataatatga aaaagatgaa tataaccaaa
     6061 agaatgtcag aatgtggggc tcttgcaatt gtaagagaag aaaatcttaa ccgtgcatgt
     6121 gaaattgctg aaggatgtat aaaaggtggt ataacagtta tagaaatgag ttatacccta
     6181 aataatgctg gagaaataat tcaagggctt aataaaaagt atggtgaaac actttgtgtg
     6241 ggagcaggaa cagttttgga tagtgaaaca gctcgtcatg ctatattaca tggagctcaa
     6301 tttattattg caccaaatta caatgaaggt gtagcgaaga tttgcaatag atatcagatt
     6361 ccttatgctc caggatgtac atcattgact gaggcagtag atgcactaag tttaggagca
     6421 acttttataa aagctttccc aatatcagat ttctatggac ctaaactagt aaaggtattt
     6481 aagacaccaa tacctgatat gccagtttta gcaagtggag ggattaatat tgaaaaccta
     6541 catatatggt tagaaaatgg agttgatgta tgtggatttg gtggtttatt aactaaaggt
     6601 tctgttgaag atattgctaa aaatgctaaa aagattcgtg aaatcattaa aaatgtaaaa
     6661 aaaatttaac atagggctgt atcatagtat cttataatta ctatgataca gcctttttaa
     6721 tattattcaa atttaaaaca gtttacattt ctattatatt acatataatt tcagtaattt
     6781 tatattaatt tttctatctg gaaaaaaatg atttaaaact taagttatga tataatttca
     6841 ttaaaagaag gcgattttta gtaaatatga gaaaataatg tgtttttagg ggaaaagtta
     6901 aatacagatt taagtgaaca agaatcgata attaagatat taaatggtga atagggcatc
     6961 aaaaaaatta ctaaattaaa agttttagag ggagaatctt agcaagtgtg tgtgaaaatt
     7021 caatttcaaa caaaaaataa ttgtaaatga aatcaattta tacacgttga agattcactt
     7081 ttatcattat attaaaaagc agagtgaatt aaaagtgagt ctaaagatag gaggataatt
     7141 aaaacaatga aagaagacat tgggttacat cgaatgtgta aaaaagaaca agcaacaaag
     7201 aatgaagtta tatcagagga tattctaaaa tctatgagta ttggggtcag taaacatttt
     7261 ttagatgatt catttacaat aatatgggga aatacatcat tctatcaatt attaggatat
     7321 acagaagaag aatttttagt taaattttct agtttaaaag attattatga acagaatctt
     7381 tatcattttg aatctatgaa aaatcatttt ttagaggctt atcatcatgg cgaaaagagt
     7441 atagaatatc aaatttgcat ttcagcaaat aatggagacc ctgtttgggt aataatgact
     7501 ggtatattaa ttgaatcaca agataatgag aaatcaatgg tctatatatt gtattcaaaa
     7561 attaatgagt taaaaataaa gcaagaagaa ctatctgata tggaaaaaga gagtacagag
     7621 aattttaaat ggatgatgtc agagtatgct gggaatgttt atattagcga tatggataca
     7681 tatgaattac tatacttaaa taagcactca tgcaatactt tacaggcatc agcaaatgaa
     7741 cttattggaa gaaaatgtta tgaggtaatt caaggtagaa cttcgccatg tccgttttgt
     7801 acaaattcgt atttaaaaaa agatgacaca tatgaatggg aattttataa taaaaaactg
     7861 aaacgaacat ttataattaa agaccgtatg ttgaattggc aagggcggag agttcgtatt
     7921 gaattatctt atgatatgta tagtgctgaa tataaattag caaaaaaaga tcaggaaaga
     7981 gaatcaattt tgaaaactat tccagcagga atggttagat tagatgcaag agattatctt
     8041 actatacttt ggtgtaatga tatatttttg aatatgattg aatatacaaa ggaacagttt
     8101 gcagaggaac ttaataatca atgtggatat ttattttctg aagattataa acgtagtaag
     8161 aaactggcac aagatttgaa ggagtctggg gataatattg tatttgaagc aaaaatatat
     8221 acacgttcaa gggaagaacg tatatggact gttacattgt gttatatcag tggggaagac
     8281 agttgggatg gaataccatc tttttatagt ataggtattg acataacaaa ggaaagaaaa
     8341 cagattgaga aattacagca tatatcagag aaagatgcat taacaggaat ttataatcgt
     8401 gctgagactg aaaggcagat taagaaatat tttgaaaaaa atttaaatgt tatgggtgca
     8461 ctgtttatga ttgatacaga taattttaaa cagattaatg atacagaagg gcatatgatt
     8521 ggagatatag tacttactga gatggcatca ggtatgaaaa aaataatgcg tgacagtgat
     8581 gttgttggaa gaataggtgg agatgagttt acaattttta tgaagaatat ttcctcagtt
     8641 aaagatgcag aaaagaaagc agaagaacta ttgtatatgt tccgtcatct atttcagaaa
     8701 gaaaaaagtt ttctaaaagt aacttgtagc ataggaatag ctatatatcc aaaggatggt
     8761 actacattca aggaaatata tgcaagagct gataaagccc tgtatcaagc aaaaaatatg
     8821 ggaaaaaata attatgtaat ctacaaccaa gattatttta aagaattaga agaatttgat
     8881 tgttcttctc ttggtacagt tattgattca gaaaaacaat acgctgaata tcctgataat
     8941 cttacacgtt atatattccg tatgctttat cagactgatg atatagacca ggcgattaat
     9001 atgatacttg aggttgttgg gaaacagttt gatgtaagtc gtgtttatat ttttgaaaat
     9061 acagaagatg gtagatatac ttctaatact tatgaatggt gtaatgaagg aatctcttct
     9121 caaatggctt atttgcaaaa cagcaattat caagactatg aggattatga aaagcttttt
     9181 gaggatgaat tagtttttta ttgtcgagat atccatacat taagtcctaa actagaagaa
     9241 ttgttttcta gtcaaggtat ccactctaca cttcaatgtg catataaaga agatagagtt
     9301 ttcagtggat ttgttggatt tgatgaatgc acaggactac gtctatggtc acaagaggaa
     9361 ataagtacat tatcattgat ttctcaaatt atatcaatgt ttttacaacg taaaaaaaat
     9421 aataaactta atcaacagat tcaacaatac cagaatattt tagataatat tgaccagtgt
     9481 atttgtgtga ttaataaaga aaataatttt ttattatatg taaataaaaa atttaaaaat
     9541 acatattcta aattccaaat tggacaacct tattatcttg attctataga tattcataag
     9601 atatccatta
//
LOCUS       DAGSHT010000127         9585 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGSHT010000127 DAGSHT010000000
VERSION     DAGSHT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.131"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(100..969)
                     /locus_tag="MJB72_002628"
     CDS             complement(100..969)
                     /locus_tag="MJB72_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBY8263712.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
     gene            complement(1273..1743)
                     /locus_tag="MJB72_002629"
     CDS             complement(1273..1743)
                     /locus_tag="MJB72_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBY8263713.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(2341..2730)
                     /locus_tag="MJB72_002630"
     CDS             complement(2341..2730)
                     /locus_tag="MJB72_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8263714.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(4387..5793)
                     /locus_tag="MJB72_002631"
     CDS             complement(4387..5793)
                     /locus_tag="MJB72_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263715.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(5838..6545)
                     /locus_tag="MJB72_002632"
     CDS             complement(5838..6545)
                     /locus_tag="MJB72_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263716.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            6716..7630
                     /locus_tag="MJB72_002633"
     CDS             6716..7630
                     /locus_tag="MJB72_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263717.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            7630..8391
                     /locus_tag="MJB72_002634"
     CDS             7630..8391
                     /locus_tag="MJB72_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263718.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            8403..9140
                     /locus_tag="MJB72_002635"
     CDS             8403..9140
                     /locus_tag="MJB72_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263719.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPTVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         3289 a         1510 c          995 g         3791 t
ORIGIN      
        1 aagatagatt ttttaaaaac actggataaa taggtaaaaa aataaacagt tagtaaaata
       61 aatttttact aactgtttat ttttattgtt taatatgtcc tataaatcct ctaagtaata
      121 ctttccttgc aaaaattctg attctaaacc tagattttta aaataatcaa ccatgcccat
      181 acacgcaaat ttttctgtag aataatgagt agcacctata acattaatta aattatcttt
      241 tgctatttga tggaactcta atactggttc aaaatgtttt aaaggcttag taaacccagt
      301 aatatataga tttataccaa gttcagcaac ttctcttgct acaaatggat aacttcctcc
      361 ccctgctgca atagcaacta aaccatcttt taatacagaa tctccataat catacaattt
      421 cacatcatga ccaacagatt ttttaattaa ttccttaatc tcttctattg attttagtga
      481 tgttttacat ataactccta cctcaatttt atcgtcatat ttacaaaatg gtttgactat
      541 ttctaaaccc agagcttttg caaaactaac agaagttgaa tagtcactat agttgtctag
      601 tggagaatgt aatacataaa aagaaattct acgatttttc atctcttcca tataagatag
      661 tggtatgtcg tgaaatggaa aaccttcatc tgatgctatg tatcccattg catgatgtga
      721 aaaaattaaa atatcttttt catctttatc tataacttgc ttaattattt ctttatcagg
      781 aaaagtagtt gtgtatactt tattaatatt ttgagcatta tctaatacta atcctatgta
      841 tttttttcta aattctggag taataaaatc attttcaaaa ttcataaaac tccaatcatc
      901 attaatattt ttaatattaa attctaaatc taatttttta taaagttcaa ttgccttaat
      961 aataaccact cctttataat aatattgaat tactacatat taatatagaa atttccgata
     1021 ataaacttta tacgtttaag tattcctact tcaaattaaa aatattataa atattcaaat
     1081 ttattaatat attttaatat tatatttatt ttttctttat taatattccc cttattttta
     1141 tttatttttt ctttattaat attttcttta ttttaattta atttatgaca tttgtcaaat
     1201 actagtccta tatattatct tgaattagtt ctattacaat taatttctac taaataatta
     1261 taatttaaac aatcattcct tactattttc acactgcttt acaaatttaa ttggatgttg
     1321 aattatttta gaagtttctt ctacagagta tccctcagat aaatatcttc tagctataac
     1381 ttccatagac tctcctatct caataatatg cttatccggg tcataaatac gcactactct
     1441 ttgttgccat tcatgtttta gtggcttatg gacaaactct acatctttat aataattgag
     1501 tttttcaata aattcgtcaa aatcatcaac ctcaaaataa agttccatat tatttgattt
     1561 ttgtataatt gtatctggat taatccctgt aagccatgcg aagtcttctt gtatagcaaa
     1621 tcctccttta aatgttacat ttttacctaa gtccaattca atttcttgct caaataattc
     1681 tttgtaaaat ttttttgata cttcaatatc cttcactgct aataatgaaa cctgatattt
     1741 cataaatttt cctcctaatt ttttatttct attttttcag tatcttctat taacattttt
     1801 ttctctatct cttcagtacc ttttattaat cctttatttt tactttttta gtatcttcta
     1861 ttaacacttt tgtttctacc tcttcagtat cttatatcaa tcttttattt tttcaatatc
     1921 ttctgttaac acttttatct ctatctcttt aatatcttat attaatcctt tatttttatt
     1981 ttttcaatat catcattaat tcctatttat tttatcatct taaatacgaa aacactgtgt
     2041 catatttgca aaatatatat tatcatctat attaaagctt cacaatttat atttaaaaat
     2101 tctctttaat tacactacct ctatttaaaa agctacctta attatactta taagtaaagg
     2161 tagcttttta aatttaatta aatagaatat ccactattaa tttttattta atttatttag
     2221 attcacatta tagtatcata atccacttat aatcatttgt catattactt aagtagacta
     2281 atatattaca taaatacact tacatacact ttatattgat gctataagaa atacgtttgc
     2341 ttatacgtct ttctcctctt cttttagatt ttttatccac tcttcaataa gtaagatatc
     2401 atctttgctc aaattaatat tttcatgtaa tgacaacaat aaacttttta tagaagaatc
     2461 atgaatattt ctaaaaaaat ctcttgtctc aactcctatg tattcctttt ctttaattaa
     2521 aacttcataa tgcgtatatt tatctatctt ttgagaattt agaaatcttt ttactactaa
     2581 tctagataaa agagtcaaag ttgtcgtttg tttccattga tatttttgct ccattcctaa
     2641 aacaatatct ctagatgtta ctttggaatc tgatttccat ataaatttca ttatctttaa
     2701 ttctgattgt ggcaactttt ttatagtcat gataaaacct tgctactcag aatatagaca
     2761 agcaatatcc ataataaaat ttttcgatta aatacatctt atacggattt atataagctc
     2821 agacttataa gatatttttc aatcttaatc ctaaatttag ctatttgtac acggtaaata
     2881 tatataaata atgtcaaaat tctcttgtat tatattgctt aattttatga aatattttgt
     2941 actcagctat ctattttgga tacttgtcca gtagctctcc tttcttttaa actctttgat
     3001 tttaaatatt tgtatttcag taaatcctac ttcctaaatt ttttatttac tacaacttat
     3061 tactgaactt cctacttcct aaacttttta tttactacaa cttattactg aacttctcac
     3121 ttactaaatt tcttacttac tataaattat tactaaactt cctactttct aaatttctta
     3181 cttactataa attattacta aacttcctac tttctaaatt tcttacttac tataaattat
     3241 tactactaaa cttcccactt actaaatttc ttacttacta taaattatta ctaaacttcc
     3301 tactttctaa atttcttact taccacaaat tattactaaa cttcctactc cctaaatttc
     3361 ttacttgcta taaattatta ctaaacttcc tactttctaa atttcttact tgctacaaat
     3421 tattactaaa tttcttactt gctataaatt attactaata aacttcccac ttcctaagtt
     3481 tcttacttac tactacttat aactaaactt cccacttcct aaatttctta cttactacca
     3541 cttataacta aacttcccac ttcctaaatt tcttacttac taccacttat cactaaactt
     3601 cccacttcct aaatttctta cttactacca cttatcacta aacttcctac tttctaaatt
     3661 tcttgcttct taagaataat gttaatttta attttaagat tatagttttt accttgttta
     3721 attgaaacat tctatataat tatatccttc tattttagag tttttttatt tatatattac
     3781 aaagtttaaa ttttttcatt aattatatta taaattttcc atatatttca acataaatac
     3841 atatatatat atgttatttc taaaaatact acacaaaaac tctactttag tagaacttaa
     3901 tggcgcaaaa tacttcaatt taaaacattt atcgtacttt aaataaataa ctataataat
     3961 atacttacaa tagttacata gaagctatta taactacaca gaacagcgta taattaaaac
     4021 ttttttagga tgtcattata ttttttctta aaattaaaaa acactatacc ataattctat
     4081 tttagtagaa tttttttgtc aataataaaa ttaaatatta actaaaaaat aaaaaaaatg
     4141 gaattatcta aagaatttaa taaccttaca ataattccaa aatattattt ctatgaaaat
     4201 tttgatacca tgagtaactc agccataagc gcataaagca caagttactc tttactttaa
     4261 taaaacttga caagaaaaga atttatcctt attctactct aatagagtta atttgtcaat
     4321 aaagaataaa attattttca aaatattttc attcttcatt cttatctgtt attattttat
     4381 aaaatatcac ttaattagag gtaattttag tattgttttt agtccctgac tatcaatgct
     4441 cgtaatatct aatttacctc tatgtgcatt aataatgcca gatactatca aaatacctag
     4501 accatgattt tgacctttgg catatgcctc acttagtcca ttttggccct ttaataaact
     4561 attaattctt tcttgactca taccttttcc atcatcttca acaattatat agcaaaaatc
     4621 taaatcagac ttagaggtaa cccttatatt acatccttgt tggttatgag ttatactatt
     4681 ttgtattaaa ttactaatag ccctttttaa taatttttta tctgcatcta taataatatc
     4741 ttcatttagt atatcaagtt caacgtaaaa gtcattatcc aaattattat ttataaactc
     4801 agaaataagc tctctcaaaa acgcagaaac tctaatctta tcacaattca atgtttgcat
     4861 attatattca agtttagaca ctaaatttag gtcatttact aattctctga ttttaatccc
     4921 ttgattgcat ataatagaca cttgctcttg ttctctattt gaaaggctac tgctttcttc
     4981 aagttctcca gcatatccaa ttatcattga aagtggagtt cttatatcat gagatattcc
     5041 tgcaatccag ttagacctag cttcctcttt cttcctaagt ttttcatctt tcatttgcaa
     5101 ctctttagat acagtattaa tagattttga aatatcactt agtattcctt tttcttgtaa
     5161 aagtactggt tctccttttg acataagctt tatgccagta ataattggct ttatagattt
     5221 caccattttt aaacctatga atagagctat taaaaaagta actattgcat taaccagaaa
     5281 catacctatt attaacatag gaaattgttc tatttcctta accgtataag taaggttaaa
     5341 cttagtgtac ttgtcttttg gataacctgt tactaatatt ccatttggat gttcccatac
     5401 atacacagga tagtccttta aaaagaacct tgaaaacttt gctacatctg tcaaagtata
     5461 atgacttgga atttcttttg gtaaatcaaa tccccatatt ctatctccat tgttatcaat
     5521 taacattgcc caaactttat ttttatgtag cacttcctta ctagaatcat ctaaaataaa
     5581 ctttccatct tttaaaacca aattatcttc aatcataatc aagtttgatt ttatttctct
     5641 gctatcactt gtatctaaat tatcagagta tttagataca aataacatat atgaaaacag
     5701 aagaaaacct gctatattta atacaataaa aagtaatgaa atcaagatac ttgaacctat
     5761 aaatcgcctc aatatgtact ttacactatc caaaattaaa tcactcctct tgatttcttt
     5821 tattctaata tctaaaatta attaatcatc aacttatatc caagacctct tactgtaata
     5881 agatgtttag gtgtagatgg agttttttct atcttctctc taacccttct aacatgaacc
     5941 ataagggtat tttcatatcc atacatatca tctccccaaa tggcttgaca tagtgaatca
     6001 ttagtaacaa ttctcccttt attttcatac aactttaaaa gcaacgaatg ctctttagct
     6061 gttaaagaaa tctcttgccc atctttctca acacatgcac tatttaaatt cacaacaaca
     6121 gaatctccca gtttaaatac tggcaactcc tcttcttcta taggcacata tacacgattt
     6181 aatataccca caagccttaa agtaagttcc tttggtaaaa atggctttac tatataatcg
     6241 tcagccccta agcccaaacc aatcaatcta tcttcatctt cacctcttgc agagagaaaa
     6301 atcactggta ttttagatgt ctctctaatt tttctcataa tagaaaaacc atctccatct
     6361 ggcaatacaa catctaaaat tgcacaatct ggctttacat ttctaaatac cctaacagct
     6421 tcctcataac ttgatgctaa aaatactcta ctaaatccct cttttctcag gatttcatct
     6481 atcattttta ataattcctt gtggtcatca accacaagta tttttttatc ttttattgac
     6541 tccataatca tcaccctcct ttagtatacc atatacccat tttcacaact ttgcctaaat
     6601 agacttttaa ggttattgta aggtaaattt cagctaattg aaatgtaacc aagttattat
     6661 actgatataa gttaaaagaa tcaataagca cagattaaaa aaggggatga aacatatgga
     6721 aatgataatt agaacacaaa gactttcaaa ggaatacaat aaaatattta gagtaaagga
     6781 tatagaccta agagttccac aaggagctgt atatggtttt ttgggtccta atggagctgg
     6841 aaaatctacc actcttaaaa tgcttcttgg acttgctaaa cccacacaag gaaatattaa
     6901 tattttaggt aaagagttaa atgaaaaaaa tagaatatca attttaaaag atataggttc
     6961 tttgatagaa tctccatcat actatggtca tcttacaggg cttgaaaaca tgaccataat
     7021 gcaaagactc ttagatttac ctaaaaaaaa tgtaaatgaa gcattaaaaa ttgtaaggtt
     7081 agaaaatcaa aaaaataaaa aagttagtca atattcttta ggtatgaagc aacgtcttgg
     7141 cattgctatg gcaataatga agtttcccaa attattaata cttgatgaac ctacaaatgg
     7201 actcgaccca gcagggattg aggaaattag agaattaata aaatctctac cagaaaaata
     7261 cgggatgact gttctaatct ctagccatct tttaagtgaa attgaccaaa ttgccacatc
     7321 aataggaata ataaatcatg gcgaactggt ttttcaagat agcataaaag aacttcataa
     7381 tagaggtcaa agtcagattg ctatcaaaac taagaatgtt gtatctgcac aaaaactatt
     7441 aaatactaaa ggatatatgt cacttataca agatgggtac ttgactattg aaaatttaaa
     7501 agactcagat gtcgctaaaa taaatggtat gcttgtccat tcaaatatag atgtatttag
     7561 aatagaagaa cataaaaaaa gcttggaaga tatattctta gaacttactg gtaaggcggt
     7621 gactttataa tgaaatacct tggtctagaa ttttataaac ttaaaagaaa gaagattatt
     7681 ctgatgacct ttttatttgt agctgttgaa ataatgtggt gtatcataaa ttcaaataga
     7741 gctattacac gtaatccaga tatgttaggt ggatttgaat attcctatat gcttatgagt
     7801 tttgcaagtt taaatggtct attctttcct atcttaatat caataattac atcacgtata
     7861 agtgatatag aacataaagg agatacatgg aagcttttaa aatcttctgt gacatcttta
     7921 aatagtattt atttatctaa atttttatgt tctgctattt tggtatctat acctgtattg
     7981 atgcaagtgt tatctattgt gttgtttgga cattttagag gggttattga gtcaatttct
     8041 gtatctttac ttttaaaata cacattagga acaattcttg taagtattgc tatcattgca
     8101 ctacacctat ggattgcaac tgtaattcca aatcaaatga ttgcaattac atttggcatg
     8161 attggtagtt ttataggttt gacatcaggt ttgttttatc aaggtattcg tagattgttt
     8221 atatggtctt attacctaga gttatcacct ttaagttatg cctatgataa tgttttaggt
     8281 tcatctgtct atagaattga tatgaatttt tctattacga ttttagtttt cttaattgga
     8341 atgctaattt attatattgg aagaaatcat ttattaaaaa aagaagtata ggagggattt
     8401 aaatgttaag gaagtcagtt ttgggagaaa ttctaaagtt aaaaaattct tttatatggt
     8461 atgtattatt agctttgcca cttataagtg tgttgattgg ttctggtaat ttttatctaa
     8521 atcaaggcat actcaaaaaa gaatggtaca gcttatggac tcaagttagt cttttttatg
     8581 gggaattttt ctttcctatc ttaatcgcaa tcttttgtgc atatatttgt aggcttgaac
     8641 atatgaatca taattggaat aatgtaatga cactgccagt taatttaaaa aatatctttt
     8701 tatctaaact tacagtagtt tcaatactta cagctattac acaagttttc tttgttatct
     8761 tttatatagt tgcaggaaat atgtttggtt tttctggtag tgtacctaaa gaattgactg
     8821 gttggattgc taaaggttgg attatttcta tttcaatagc ttctatacaa ttatacatgt
     8881 ctataaagat tagaagtttt gcaactccta ttggtattag tctttgcctt tctctatttg
     8941 gtatgggatt aatagtagca aaacctactg ttggtatatt tttcccttat tctatgttag
     9001 gtagtgctat gggggttata aagcaatctg ctttaaatgt taatgacact atagttacaa
     9061 tatgtgttgc atttattact actatagtat tttctgaaat tgccaataga aattttagaa
     9121 agaatgatgt tatttcttaa tggtaaatgc tagatatttt tgggtttgaa atttatattc
     9181 tttttagttg aaatctatat ccattttatt taaaaatata tatcttttat ttgaatttta
     9241 tatgttttat ctactcgcta tataatagat atactcctaa aattaaaaaa tatataataa
     9301 tgttattatt taaaattatt atgtattata ttaaaactta atgcaaaaac aatttttata
     9361 ttatataatg aaacttaatt aaatataggt aaaaaatcat tcaatatatt aaataaatat
     9421 ttaactttaa taatctatat ttaattatag ctataacata gaattatcaa atatattgaa
     9481 tgaatatttg actcttggaa tgtgtaatta aattaagttt ggttattcga tggaaggcaa
     9541 atttgatata aaactccatt taaagtgcca gataaaaact ttatg
//
LOCUS       DAGSHT010000128         9472 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.132, whole genome shotgun sequence.
ACCESSION   DAGSHT010000128 DAGSHT010000000
VERSION     DAGSHT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.132"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            154..1221
                     /locus_tag="MJB72_002636"
     CDS             154..1221
                     /locus_tag="MJB72_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBY8263720.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELKGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1240..2400
                     /locus_tag="MJB72_002637"
     CDS             1240..2400
                     /locus_tag="MJB72_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBY8263721.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELDDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2413..4509
                     /locus_tag="MJB72_002638"
     CDS             2413..4509
                     /locus_tag="MJB72_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBY8263722.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4668..5012)
                     /locus_tag="MJB72_002639"
     CDS             complement(4668..5012)
                     /locus_tag="MJB72_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY8263723.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5304..6194
                     /locus_tag="MJB72_002640"
     CDS             5304..6194
                     /locus_tag="MJB72_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY8263724.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFLSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6412..7698
                     /locus_tag="MJB72_002641"
     CDS             6412..7698
                     /locus_tag="MJB72_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY8263725.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7787..8230)
                     /locus_tag="MJB72_002642"
     CDS             complement(7787..8230)
                     /locus_tag="MJB72_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBY8263726.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(8254..9048)
                     /locus_tag="MJB72_002643"
     CDS             complement(8254..9048)
                     /locus_tag="MJB72_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBY8263727.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         3647 a         1081 c         1606 g         3138 t
ORIGIN      
        1 agagtattaa ttgaattaag attatcacat tatattttgg aaaagagtaa attttcagta
       61 aattatcttg acatatatat tgaagtaata atataattta aagtataaaa ttttaaaaat
      121 tgaattataa cttcaaaaaa taaggagggt aatatgaaga taacagatat caaatttgaa
      181 aagcttagaa ttaaattaaa aaaacctgta gtagtttcat ttggagtaat tgagtatgga
      241 gaaagtataa ttttaaaaat tgaaactgat gaagggtatt gtggatttgg cgaagcagca
      301 cctcttgcag cagttacagg tgaagttttg gataatgttc tttcagtatt gcttatgttt
      361 aaaaaagagt taataggcaa agacccacta gatattgaaa ccatacatac catcatggat
      421 ggaattatta taggtaatac ttctgcaaaa gcagctattg acatagcttt atatgatatt
      481 aaaggaaaaa ttatgaatgc acctctttat aaagttcttg gaggatttga tagtaaagtc
      541 caaacggata ttactataag tatagataag ccagaaaaaa tggcagtaga agctttagaa
      601 agagtaaaag aaggttttag gatattgaag cttaaagcag gtataaatcc tgaagacgat
      661 attgaagctg taaagttaat tagagaagcc gttggagaga gtataagaat tagaattgat
      721 gcaaatcaag gatggaacgt aaatagcagt ataaatacta tcaaaaagtt agaagaattt
      781 aatgttgatg caattgaaca agcacttcca cattgggatt tagatggtac tgcttacata
      841 agaaataaaa gtaacacaaa aattatgata gatgaatcat tacattcacc aatagatgca
      901 attaaggcta taaaaaagaa tgcagttgat acatttaata ttaagctgat gaaatcaagt
      961 ggaatatatc ctgctataaa aatcaacaac atagctgagg caagtggagt aaattgtatg
     1021 ttaggatgta tgctggaaac gagaattggt ataacagcag cagcaaattt aatagcatct
     1081 aaaaagaaca taacagaagc tgaccttgat agttttatgt tttgtgaaga attaaaaggt
     1141 atatctggag gatttgtaat ggatggagat attatgaatc ttgtcaataa accaggttta
     1201 ggaatagaag taaacttatg ataaatttgg aggttaaata tgaatacact tttttcaaat
     1261 ccaggtagta tacttgctgt aatgacatca atgattgcat tgggttttta cttacaaaga
     1321 tacaaagcca ttaaaagctt aggaccagca cttacaataa tcataatggg aataatactt
     1381 tcaaacttaa aagtagttcc agtaagtact gagttatatg gaactatttc aacatatgct
     1441 atcccagttt ctatgactat aatgttgatg agtgttgact taaaagaaat gacaaaatta
     1501 tcaagagaac ctttaattgc aatttttgta gctgtattaa ctgtaagtat catggcattt
     1561 ttatttggat tagtatttgc agaaaaaata tcagaaggtt ggaaggttgc tggaatgttt
     1621 gtaggtacat acactggtgg aagtgctaat cttacagcta ttggaacagg tcttaatgta
     1681 agtagacaaa ctcttgcagc agcaaatgca gcagactatg taattggagt accaacattg
     1741 atatttatgt ttgctctacc agcaatatta aaaaattcaa agaagtttaa aaaactttgg
     1801 ccatatcatg tagaagaatc agaattagat gattgtcaaa atgaagaatt tatggagtca
     1861 aaagaatgga gtattaaaga tatagcttgg atgttggcta taggttttgt tgttacagag
     1921 gtttcaacaa tacttgctgg atatttcaat tcaagtttta gtagtgcagc aagaatactt
     1981 ttagttacaa caatttcaat tataattgct caattaaagc cagtaaagaa actaaaggga
     2041 aatttagatt taggattatt tgttgcatta ttctttttat gtacaatagg tttttcagta
     2101 gatataaaag agtttttagg ttcaactttt actataacac tttactgttt tagcataatt
     2161 tttgcttcat ttgttttcca tttaggcata actagacttt taaagataaa gtatcaatat
     2221 gtaatacttt ctatagtagg agcaattgct gatggtccta cttcagcttt agtagctgct
     2281 tctgcaaagt ggaattcgct tgtaagtgta gcagtcgtaa tgggagtaat tggtggagta
     2341 ttaggaaatt atgcaggtat atcagtggca tatgcaataa agatgtctct aggattatag
     2401 gggggagttt aattgataaa atttaaatta tacatatctg gactatacag tggaaatgta
     2461 atctttgatg gagatttatt aattgaaaag ttgaatcctt ttacaaataa aatagaaagt
     2521 ttaaaaccaa taagcaaaga agaaaataca tactatttaa atcttacaaa aatagattta
     2581 aagtctttat ttaataactt tgatgtatat actaaaagct tagtaaacac agataaagac
     2641 acagttataa ataacttagg agaaacgcat tctaaactta atgaatatat atgggttcaa
     2701 agaaacaaaa agttcccact agatataatt attgtagata ataaaatagt aggttttatc
     2761 tgtttatcca gagaaacttg tactatatta attatggatg gatatgaaga gtatactgta
     2821 ttaaaagaat gggaaaaaac tcataaaaat gaagagatat attctataag atttggtgga
     2881 aattatatga ttgatatgaa agatggtata aaactttcta cagatgtata tctccctgat
     2941 tttgtagatt ctaccaaaaa agcaccgaca attcttatga gaactccata tggaaaagaa
     3001 aatgataaag aaatatatta taaatatgta caaagaggat atgctgttgt aatacaagat
     3061 gttaggggaa gaaatgaatc agaaggtaag tgggaaccta tgattcatga gagggaagat
     3121 ggagatagta ccataaattg gatagtatca caggaatggt caagtggaat agttggaatg
     3181 cttggagcat catatcttgg ttatgtccaa tgggcagctg catcttcagg caataagcat
     3241 cttaaagctt tagttagtat agtaacatca ggaagccctt ttattgatat accaagaaaa
     3301 ggaggagctt ttgtatctgg tatgcttgca tgggcattta tggtatcaag aaataaagtt
     3361 gatagaagca aaatggtaag agatgattgg gatgatgttt taaacatacg accaatagaa
     3421 aatatacctg ttgaagctct tggatataga attgaatttc tggaagaatg gttaaagaga
     3481 gtagaaaaag atgagtattg ggatttgatg gattggcatt tacaaaaaga taaaataaat
     3541 gtacctgcat tggttgtatc tgggtggtat gatgataata gtatgggaac tacagaggcc
     3601 ttagatgtta taaaggacta tgaaaaagga aagagaaagg ctattctagg tccatggatg
     3661 cataattcaa atactttaag agatataaat ggtatttctt taggaaatag ttcattaaga
     3721 tatgatttgg attataatta tctattatgg tttgataaat acttaaaagg tatagaaaat
     3781 aacatagata cgactgctcc agtagagtat tactctgtag gttctaataa gtggaagaca
     3841 gaagaaaatt ggccaataat aaataagata gataaaagta tgtatctaat aagtgatgga
     3901 aatgctaaca catctttagg aaatggtaga ttagtatttg ataatggttt agaagaaaag
     3961 tatgatagtt atatatataa tccaaaagac ccatcagtac aactaattga tatgtcagag
     4021 aatgaagttg gagttccaaa taactacaaa gatttagaaa aaagaagtga tatgttgtgt
     4081 tatacatcag atgcttttag tgaagaattt actgtaacag gtgatataaa gttagagttt
     4141 tttgcatcaa gttctgccaa agatactgac tgggttataa aaattatgga cgtggattta
     4201 gatggaaatt caattaaatt agctgatggt atacttagtg ctagatttag aaatagtttt
     4261 tataagtcag agtttatgga agaaggagag atttataagt ttacgattat aacttcaaaa
     4321 atatcaaaca cgtttaaagt tggtcataaa attagactag atataacgtc tagtgctaaa
     4381 aactttattt tccaaaatag caacacgaca gagggatata atagtattga atatatagaa
     4441 gcaaagaata cgatttatca tggaggtaaa tatccatcta aattgatatt gccaatagag
     4501 aataagtaaa tttatataga ttaaatagtg aggaggtgtc tcaaaatgaa ttttttagtt
     4561 cgtgagacac tttcttttat acaaaatcaa atgtattttc atcatttcaa caatgaaaaa
     4621 gaggcttatc tctctgtttt gagatagcct cctataaact aaattgatta ttttgtagct
     4681 gcaacaacgc tcatttcaac taacaaatgc tctctagcaa gtctagcttc aacacaagct
     4741 ctagcaggtt caaatccttt ttcaacccaa gcatcccaaa cagagttcat agcttcaaag
     4801 tctttcatat ctcttaaata aatagtaaca gatagcaaat gttgcttatc tgaaccatat
     4861 ttgtttaata aatcttctat tttagctaaa acttcagttg tttgttcaat tactcctcct
     4921 tcagcatgag tttgtccaca taaataaact gtattgttat gaactacagc tctactcatt
     4981 cttccagttc cttcatatct ctttatttcc atattttaaa tacctccata gttaaaaaat
     5041 ctattaaaaa tctaaataat aaattaaaaa aagtctatac tatataaaat tatattatat
     5101 attctgataa taatccataa aaacatataa ctttttagct ataattatta ttgttattaa
     5161 ttgaaaagat ttattagtta cttgacatag tttacacttt taattatagt ataaattgga
     5221 atatttagat aattattatt taatgtataa gctaagtttt taattaatat ggctttatac
     5281 atttatatag aaaagaggta cgtatgagta gcacaatcaa atcaaatcga ataaaaggta
     5341 taatgtttat aatagcatca gcgcttggct ttgcaatgat gtctgcattc gtaaagctag
     5401 ctggagattt accaagtttt caaaaagtgt tctttagaaa tctggtatca gctataatag
     5461 ctttttggct tatcataaaa cataaaggaa gtctaactgg taaaaaagaa aatttaaaag
     5521 tacttttata taggtcaata tttggaactc taggagttat atttaactac tatgcaattg
     5581 acaagttgat gctttcagat gctaatatgc taaataaaat gagtcccttc cttgtagtaa
     5641 tattttgtgc aattgctcta aaagaaaaaa taaatgtaaa gcagatagga gctattataa
     5701 ttgcatttat aggagcttta ttcataataa aaccaacatt tagtgtagaa gtaattccat
     5761 atcttggagg aattgcagga gctgtatttg cagcaatggc atatacttgt ttaagagttc
     5821 ttggagatag agaagattac tatacaattg tatttttcct ttcagttttt tcattagtta
     5881 ctgtagggcc aattgctttt gcagtatatg aaccaatgac ccttatgcaa cttatatatc
     5941 tattactagg tggagtattt gcaagtctag gtcaatttgg aataactcta gcatataaat
     6001 atgcacctgc taaggaaatc tctatatttg actatagtaa tataatcttt tcagcaattt
     6061 taagtatatt cttattcaat gtctatccag atatgttaag tgttgttgga tatttaatag
     6121 tattttcggc agcattttat atgtttttat acaataagaa gttagataaa ttagacaaag
     6181 aaaagaagaa gtaaactaga taaaagtgtt tttataattt gtattgaata tttaagtctt
     6241 tgtattaaaa ttaaagaaaa tttaattaaa aatgtttggt atattaactt ataacagcag
     6301 ttaaaattga aaaattataa ctgctgtttc ttttattaaa aatatccaaa tgtctgtaaa
     6361 aatagactat aatagagtag tataaataat ataaagggga taaattaaag tatggaaaaa
     6421 gtgaaagttg ctgtgattgg agcaggtaat agaggaacgt atgcatatgc tccatatatt
     6481 tatgaaaatt ctgatgtatg tgaaatagtt gctgtagcag aacctaaaaa aggaagacga
     6541 gaactattta cacaaaaata taatctagat agtaaaaatg tatttgaaac tttagaagac
     6601 ttttttaaac atgacaagat ggcagatgca gtaataatag ctacaaatga tgatagacat
     6661 tatgatgtag caaaactagc tcttgaaaaa ggttatcatg tactattaga aaagccaatg
     6721 tctaatagtt tagatggtct ggtacatata gatgacttgt gtgataagta taaagataag
     6781 atttttatga tttgccatgt actgagatat tctccatttt ataataaatt aaaagaaatt
     6841 gtagaaagca aaaaattagg agagctagtt agtatacaat ataatgaaaa tataggatat
     6901 tggcattttg cacatagctt tactagaggg aattggagaa atagcaatga aaccagccct
     6961 ttaattttag caaagagttg tcatgacatg gatattttgt tatatttagt aggaagtaga
     7021 tgcaaaaaaa tatctgcatt tggaagctta aagcatctta ataaccaaaa tgctagtgga
     7081 gagatggcac aaaattgtct tcagtgttta gttgaaaaaa aatgtccata ttcagcaaag
     7141 agaatttatt tagaaaaaga tagaagtata aatagagctg ttcacataaa tccaactgaa
     7201 gagaatctat taaatatact taagacaagt ccatatggaa gatgtgttta cagatgtgat
     7261 aataatgttg tagacaatat ggtaaatata ctacagtttg aaaatggagt gacggctact
     7321 tttaatttat gtgcatttac taaagaaaat ggaagaacta taaagttaat gtttagccat
     7381 ggtgaagttg gtggggattt aaataaaaat gagataagaa ttaaagaatt tggaaaaaac
     7441 gaagaaatag ttatgaatcc atcaaatcaa cagaatatgg tagaggaata tgatagaaat
     7501 cttatagctg aatttattaa attagtttca aataaagaac tagaaaaggg tagagtagca
     7561 gcaaaagaag ctatacaaag tcatgtaatg gcatttgcag cagaatattc tagggtcagt
     7621 gatgaagtag tgtatataga agaatttttt gatagtgcta agcaaatgac taaggagata
     7681 gaagaaacta tattttaatt aataaaaatt tattaaataa aataaagata gattaaataa
     7741 tagaaatacg aaaatatgtg ttctattaag gaatctatct tttttattaa ggttttttct
     7801 tatccctatc aaaaaaatcc atacattctg ataaaagata agaaagtaaa gcgaataaga
     7861 tttctgtact taaaggaatc gttactgaat ccataaaggt atcaattatg ttcatagcta
     7921 gaaaagttag tataaaatca attataaatt cgcaaagttt atatattgag tatggtaatt
     7981 tgtttacata tctgaatata tctaaaatag tagtgcatag aaaatcaatt ggagtggtta
     8041 tacaaaaata tattaaaaag aaaattaata cagctccaag agatttatat tcaaatccta
     8101 agaatgataa gaagccaatt cctataaccg tgaaaataca aaaagttata ccaaaaaaaa
     8161 cagcaaaaaa taaaaataca gaaataaatt cgcctaaagg aatccttctt ttattactta
     8221 tcttaatcaa ctccttaatt tatgatatga aatttatata aataataaca ttcttaaaaa
     8281 ttcataatag tcctctgttt caaatagtac tgtttgagaa tctatttttt tcatatttgg
     8341 ataaaaagag gccttaaaag ctttataatg ttctctaaat tttatttcta tcttaaattt
     8401 atctggcaat tttattaagt gcctagataa atctccagat aaagcttttt tgacaccttt
     8461 ttcaattttt tttagtgcaa ggtttggatg gattgaaatt gaaccatttc caattccttt
     8521 tgacacagga acagtgacta tatttggatt tagttttttt gcactctcac aaagcatttc
     8581 atctccactt aaaaatgcta caggaacatt ataatatgca gcaatataag catttatagt
     8641 aaattcagat gctatttcat catttatttt aaaatagtca taatcaatat ccatagtatg
     8701 ggaaagtgga ttaccattac aagtagctcc agagtgatag ccagtgaata tacatgcatc
     8761 aaagctttca tcaattccag ccatcataac tagtgggtct ttggtccatc ctctaagtat
     8821 tttgacgttt tcaggtaaaa tatttggatt aatatttcta ccactgtcat gtgcatcttt
     8881 aacaagtatt tcatgtacac cagattgagt agctccatca catgctgctg ttatctcttt
     8941 actcatttgt gttctatagt gttgcgaaaa ttcacattca agttttgttt catcccaatt
     9001 tacaatacca caagtacctt ctatatcagc acttaaatac aatttcataa tatatacccc
     9061 cttatgttta ttattgatga taataacaga taacagaaaa tacataatca ataaagggca
     9121 taaaaatatt atgtcttact attaactatt attaaatctg taactgaaaa ataactaaat
     9181 tggtataaaa aaatataaca ctatgtgtca tttatttata tagtttttta tatttgatgt
     9241 ttatataaaa ttgtaacata taagtggtta catttactag tttaacatag ttaaaatagt
     9301 taggtattat ttttaagtta aattgattat tataaaatcg cagaataaaa gaaaaattta
     9361 atttattaaa atgcatgaaa tgttttttcc tcaacataat gctaaattaa actcaaaaag
     9421 aaataataga aaaatcttat gtagcttaga aataatgtga atgatttaag gg
//
LOCUS       DAGSHT010000129         9464 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGSHT010000129 DAGSHT010000000
VERSION     DAGSHT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.133"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(113..766)
                     /locus_tag="MJB72_002644"
     CDS             complement(113..766)
                     /locus_tag="MJB72_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8263728.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1011..2876)
                     /locus_tag="MJB72_002645"
     CDS             complement(1011..2876)
                     /locus_tag="MJB72_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBY8263729.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2881..5301)
                     /locus_tag="MJB72_002646"
     CDS             complement(2881..5301)
                     /locus_tag="MJB72_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBY8263730.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5315..6757)
                     /gene="glgA"
                     /locus_tag="MJB72_002647"
     CDS             complement(5315..6757)
                     /gene="glgA"
                     /locus_tag="MJB72_002647"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBY8263731.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6786..7916)
                     /gene="glgD"
                     /locus_tag="MJB72_002648"
     CDS             complement(6786..7916)
                     /gene="glgD"
                     /locus_tag="MJB72_002648"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBY8263732.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7918..9078)
                     /locus_tag="MJB72_002649"
     CDS             complement(7918..9078)
                     /locus_tag="MJB72_002649"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBY8263733.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3000 a         1728 c          920 g         3816 t
ORIGIN      
        1 ctatcgtagt gccttagagt ttttatttgt atttaaagtt aaatttattc ttgtttttcc
       61 tttaatattt aatagtaaaa caacaaaatt ataaaaaaaa taaagagaaa ctttataaaa
      121 gcttctcttt tttcatctct gctataggta tcgttttcca atgttctctc aaaactcttt
      181 caacattttc taaatcattc tccctcatag attttactac ttcaatatgt tgattattgc
      241 tgtatgtcaa cattttatat aactcatctt cattatcact agtatatgac tttgacataa
      301 aattatatct taaagagttc aatagattta ctaacacctg attatctgat ttagttatgt
      361 atatatcgtg aaattcatta cttaatttta aatagctatt gtaattttta tactttatag
      421 agaccgccat catttctgtt aattcttcca tttttactag gtctgattca gttaaatttt
      481 gtaatgctga agaaccggct aacgcatcca atactccaat tacggaatag gtgtctaatt
      541 tctccttttc ctgaaattct tttactacaa atcccttatt aggtattttt tctagtaaat
      601 tttcacctgc taacctagtt aatgcttctc tcgctggtgt tctactaact ccgatagatt
      661 tacaaatctc agattcagta attctctgat tagcttcata ttctccattt tggattttct
      721 ttgatatata gtcatatact tgagttgtaa gtggatctac cttcataaga cgttcctcca
      781 tattaagatt ataaaagtta aatctcccta caactttggt agagagttca gttttatctt
      841 actaatttat ataatatcac aattatgata ttttttatac ttctttgtaa tattttgaaa
      901 tactaaaatt cttatgataa tttatacaaa aaaagtagaa aaagttttga taaaacaaaa
      961 ctctttccac attcacctta ttttctattt atttttctaa aaaaagatat ttaatttact
     1021 ctaaaaattc taaatgattt agagtctaaa tctactttca actcattatt ttcaatttgt
     1081 atagtactat ttgcattttt aagactatac tttataggta tttcctcaat aaaactactc
     1141 tctatacata atgatatact ttccacattt tcacttctat ttactaaaat taacattcta
     1201 tcattatttt cattaactct aataaaagaa aatacagaat cattattagt atatataaat
     1261 tctgtatttc cagaacttaa aagtttattc ttatttctaa tttttataac atctctataa
     1321 aaattaagca tatcttcgtc ttcatttttc catggataag ttcttctatt atctgggtca
     1381 acacctccac aaagacctgc ttcatctcca taatatatat atggtactcc ttcaaatgtc
     1441 atctgtatag ctacagcaag cttcactaaa tctacatcat catttaattc cgttttagct
     1501 cttgtaacat catgtgttcc aattaaattt aaattagacc taaatgattc ttttggataa
     1561 ttttctttaa tctgcatata tctattacaa agttcatatg aattaatttc tccttttaag
     1621 aaagaaaaca tgttatttct aaaaggataa cccataacag aatctaattc ttcacctaat
     1681 aaataacttc ttctctgacc atagctaatc ttatttgatg catcttccca tacttcacct
     1741 ataagaattg agtcatcatc tgcctttttt aattcctttt ttaactctct tataaactta
     1801 gttggtaatt cgtctgcaac atctagtctc cagcccttaa tacccatatt tacccattta
     1861 tttataacgc tatcattgtc atatattata taatccatat agctatgttc taattcattt
     1921 atatttggca atgtcttaac atcccaccaa cttttatatt tttgaggaaa ttcttcaaac
     1981 atataccatg aataatatgg agattcttta gattgatatg cccctaaact attatagttt
     2041 ccatacatat taaaatattt actatctgct ccagtatgac taaaaactcc atctaatact
     2101 attgatatac ctttttcttt tgctttatca ataagttctt taaatgtatc ttcatcccca
     2161 aacattgggt caattttttt atagtcacct gtatcatact tatgattact agaggcttca
     2221 aatatagggc ttagatatag tatacttaca cctaattttt ttaaatatcc aagtttattg
     2281 attatccctc ttaaatttcc accatggaaa tcccatctta taacatcccc ttgactatcc
     2341 tttatgtaca taggtgtatc ttcccaattt ccatatatga aactattttt tttaggatta
     2401 tcaaccttac cactacgatt tccattattg aatctatcta caaatatatg ataaagtatt
     2461 ccttctttgt accatgttgg tactttaaaa tctttgtaaa cagtgatttg atatttatta
     2521 atatcactat aattatattc acaagaatat ccattatctc tgttttttcc ataaaataaa
     2581 gtctttatct gaccatccat ttcaacatca actttaaagt aataaaaata aacacctgta
     2641 ccatcaaact tttcaatctt accacaaaag cactttccaa gtttatcatt atctaattct
     2701 tcatttaaag ttattgttct catttcttct tctctattta tttctaggga aatagacttg
     2761 atattgtatc cctcatttac tttaacacaa atggtcaatt cctcatcaaa ttttaaagct
     2821 ccaaacggag ccttaaaatt tttatcccaa gagttatatt ctattacact ttgcatttaa
     2881 tcacaccctt ttaaaccaaa tgtcttttgc atactcagaa attgttctat cagaagaaaa
     2941 aaatccagag ttagctatat taactaaaga cattttattc catttttctt tatctctata
     3001 taacctgttt atatctgctt gagcttgacc ataattttca aaatctctca atacaaaata
     3061 ctcatcattg taagtagtga gtgaattata aatacttctt ccttcttcgc caagattagg
     3121 aataaatcca tttattaaat catctactac cctttttata tccatattag aatgatatag
     3181 gtcaagtgaa gaatatccac catatttatt ataatttagt acttgttctg cactaagtcc
     3241 aaataaaaac atattttctt taccaacctg ttcacatatc tctacatttg caccgtcaag
     3301 tgtagccaaa gttatagcac cattcatcat aaacttcata ttacttgttc cagatgcttc
     3361 cttagttgta gttgaaattt gctcactaac atttgctgct ggtattatta tttctgctaa
     3421 tgatactcca taattctcta aaaatactac ttttaacttg tcctttactc taacatcatt
     3481 atttatagta cttgcaacag aatttataaa tttaattata catttagcca aataatatcc
     3541 tggtgctgct tttgcgccaa atataaaagt tcttgggtca agattcaaat taggattatc
     3601 aagtaattca tgatacatat gaagtacatt aaatatattc agtaactgtc ttttatacgc
     3661 atgcaatctc ttaacttgaa catcaaatat cgaacttggg tctacattta aatcgtattt
     3721 atcttttata aactttgcta gatttgcttt attattgtat tttatatcat caagtctttg
     3781 taaaacagag gaatcatttt taaatttttc aatatttttt aattgtaatg tatctttctt
     3841 ccatgaatca ccaattaaat ctgtaattaa attgctaagt tgaggatttg aacttataag
     3901 ccatcgccta tgtgcaattc cattagtctt attattaaat ttatttggtt catcttgata
     3961 gaaatctttt aatacctctg tttcaagaat atgagtatga agcttagcaa ctccatttac
     4021 actatgactt gtgacaatac ataagtttgc catattgata ttgtcacagt ctattatact
     4081 cattcttttt attctatctt ggtcatatcc tttattattt aattcctcta catatcttct
     4141 atttatttct tctattatca tataaattct tggtaatagc tctttcatca tacttacagg
     4201 ccatttttcc attgcttctg acattatagt atgattagta taggacactg tttttttagt
     4261 tatttcccat gcttcatccc aagataaatt ttcttcatca agcaaaattc tcataagttc
     4321 tggtatgcat aaggtaggat gtgtatcatt tatatgaatt gctaccttat cgaataaatt
     4381 attaatattt aatttgttct ttttatattt tcttattata tcttgtatcc ctgcacttac
     4441 aaaaaagtat tcttgtttca acctcaacaa cttacctgca taatttgaat catcaggata
     4501 taatacctgt gatatttcct cagtataata cttatgtttt aaagcctctt catagctacc
     4561 agaataattt agagcatttg aggtaagttc accaaaatct ctctttggta tttcactctt
     4621 aaacaatctt aaagtattta tacattggtt ttggtatccg attattggta tatcataagg
     4681 cattgccatt acaggaaggt agtttttatg aattactttt aaatgactac cttccttgat
     4741 taattctacc tcaccaccaa acttcaccat tgttgcttca tttggtctta cagtttccca
     4801 tgcatatcgt ccttctgtaa gccagttatc aggcacttct acctgataac catttacaaa
     4861 tttttgttca aacagaccat atttatacct tattccatat ccttgaccac ttatgttcaa
     4921 agatgccata gaatctaaaa agcaagctgc taatctacca agtccaccat ttcctaaggc
     4981 tgggtctact tcagcttcaa ttaaatcatc caagttgatt cctaattcag ataaaccttc
     5041 tctaacttct tcttcaatat ttaaatttaa taaattagat tttaactgtc tccctattaa
     5101 aaattctaaa ctaaaataat aaacttcttt tttcttatct aactttgtag caacccattt
     5161 ttttgctatc tcatctttca atagactaca taatacattt aacaattgtt catcagtagc
     5221 ttccttgata gattgtgcat ataagctata catttttact tcaaattttt gcttgaattt
     5281 ctttttgctt attgttacca aaaaaaccac tcctttaatc acatatgtct ctatatgttt
     5341 ctatatactc cttagctgat tttttccaac tattatctgt tttcattgca ttttcaacta
     5401 atttaatcca tttttctttg tccttaaata ctttgattgc atttttcaaa cagaaaaaca
     5461 tctcatgagc attgtaattt ttaaaactaa atccattgcc ttctccagta aatttattat
     5521 aaggtgttac tgtatctcta agccctccag tctctctaac tattggtaaa gaaccatatc
     5581 tcatagctaa catttgacct ataccacaag gctcaaataa tgatggcatt aagaacatat
     5641 cacttgcagc atatatctgt tgagccaatg atgcattaaa agtaattcta gctgatactt
     5701 tatctgaaaa attagaatcg taatagttga acattgattg atattgatgt tctccagttc
     5761 ctaaaacgac aatctgtaaa tcttctctca taagttctgg catcatgtat gctattaaat
     5821 caaatccttt ttgagaaact aatctcgaaa ctatacctat caacggaata gatgaatctt
     5881 ttttaagtcc caatatatct tgtaatctca atttattttc tacttttttg tcaattgagt
     5941 ctacatcata atgaacaaat atatctttat cttttgcagg gtcatttaaa tcataatcta
     6001 taccatttaa tatacctttt aattttcctg actcttttct aagtaatcca tctaattgtt
     6061 caccataaaa agatgtttgt atttcatttg cataagtagg actaacagtt ataattttat
     6121 ctgcaaaatt tataccagct ttcataaaat ttattgagcc ataatactct atatctccat
     6181 tatcaaagta tttttgtggt aagtccaata tatcatataa aacatgctta tcaaatacac
     6241 cttgatattg gagattatgt atagtataca tagtttttat atttttatac ccctcaaggg
     6301 ttgcatatct ttcttttaga attaatggta acatccctgt atgccaatca tttatatgta
     6361 ttacatctgg ataaaaatca attctactta tagcttctaa aactgcatct gtaaagaaag
     6421 tatatctttc tgcatcatct ccatatccat agatactctt tctttcatct tctcttctaa
     6481 aataaaattc attatctata aagtaaaatt ttacaccatc taattccatt tctaaaagac
     6541 cacagtattg atttctccat ccaacacgta cactaaacac agctatttct tttagttgat
     6601 cttttaaata acttggtata gttgaatatt tgggcattat tacccttaca tctacacctt
     6661 ctttttttaa ttctttaggc aatgcataag aaacatctcc taatcctcca gtttttgcaa
     6721 agggccaaca ctctgctgtt acataaaaaa ccttcattag tcattccccc ttctaaaaaa
     6781 tttatttata tatttgtatt cttttccact acaagaggat atttttcatc tcctcttaat
     6841 tctttcccat ttgatacact cgtattttta tctaatattg cattatatag ttttgcattt
     6901 gactttatta cacaattttg cattactatt gagttctcta taactgttcc tttttcaaca
     6961 tttacctttc taaatatgat actatctttt acagtacctt ctattaaaca accagatgct
     7021 ataaagctat tttctacttt agcactatct gtataaattg taggtgattc attcttttct
     7081 tttgtaaata tctttctttc agaatataat aattcatctg caacttctaa atttaataaa
     7141 tctttgctca tttcaaaata tgattcaaca gaatttacac attttagata tcctgtatat
     7201 tcataggctc ctattttcat gttttctgct tcttcaataa taaattcttc taaataacaa
     7261 taattgcctt tatttacaat gttatatata cattcaacaa aatcttcacg cttcataata
     7321 tatgtctcca tacatatgtt tgctctaggg aacttaccta aattatttcc tacatttatt
     7381 accctattgt tactatctaa atttagagtt aagttaccag caaaatcttt atttgcatta
     7441 tttacatttt tatatattat agttatatca ttatgagatt ttttatggta ttccaatgct
     7501 tgagaataat ttaaattgca tagcatatag cttggagcta ttaaaatata ttcttcttca
     7561 ctcttttcta tataatctag attagctaag atagtatata tatcaccttc tctatgtgag
     7621 taagtatttc tatacttagt gttttctgga ctaaaaattg ataggccacc tttttttcta
     7681 cttaaatccc aatctttacc actacctata tggtctgtta atgatctgta tttttctttt
     7741 gcgaatattc caactttagt gattcctgaa tttatcatat tagataaagc aaagtctatt
     7801 attctatatc ttccagctat tggtgtagat gctattggtc ttccatagtt aagtttgttt
     7861 attgctgggt ctcctttttt gtttaaattt attatcccta aacattcatt tctcatctta
     7921 aagacctcct tctattaata cgcatctagg ttcgatattt tcatactcag aaactacatt
     7981 tatttcatta tttacattct ttatctgaac attatcattt atagttgcac actcacctat
     8041 catagctttt tctatagtta cattttcacc tattacaaca tttggcatta ttactgaatc
     8101 aattacttta cttcctttac caacaacaac tccatgtgat aatacagagt tacctacttc
     8161 acctaaaatt ctacaaccat cagctatcat tgatctatga acatttgcat ttttacctat
     8221 atattgtgga ggcattgcca tagtatttgt atatatcttc cacttagggt ctgctaaatc
     8281 aagtgtttct ggagatttta ttatatccat attagcatcc catagacttt gtatagttcc
     8341 tacatctctc cagtatcctt taaatggata agcatacatt ccaacattat cttctaacat
     8401 tttaggtatt aaattttttc caaaatcatc ataatttatt tctggatttt tttctgcttc
     8461 cttaaaataa tttctcaaca ttttccaatc aaatatgtat actcccatag atgccaaatt
     8521 gctcttaggt tcacttggtt tttcttcaaa ttcatagata cttgaatctt catttgtatt
     8581 cattatccca aatcttgaag cttcatccca aggaacttca atcactgcta ttgtagcttt
     8641 agagcctttt tccttatgaa attttaacat tttgctataa tccatcttgt atatatggtc
     8701 accagataat atcaatacat attctggatt ataggtatct acaaagtcca tattttgata
     8761 tatcgcatgt gctgtaccat tataccaatt tccttctttt tcattcataa atggttgtaa
     8821 aacatagaca ccaccattta ttctgtccaa atcccaatga cttcccatgc ctatatgtga
     8881 atttaatatc aacggtctat attgagttaa aacacctaca gtatcaatac cagagtttga
     8941 acaattgctt aacacaaaat ctataattct atacttacct ccaaatgata cagctggttt
     9001 ggcaattctt tttgtaaaaa cacctaatct tgaaccttgc cctcctgcca aaatcatagc
     9061 taacatctct tttttcattt aatgacactc ccctcatcta gtaattaata tttataattg
     9121 catttttact tttataaact ttatcagtca ataaatgtcg tgtataaatg ttgcttaaat
     9181 aataccttaa taaaaataaa ataatatcaa ctatacaatt gatataatgt ataccccata
     9241 atgaacttac cgacgtatac attattttcc ataatcacta tagtatttat taatattcaa
     9301 ttatcggtta aaaaataata caaattttat tttataaata taaaaacatt ttttataaat
     9361 cccttgtgtc atatgaacat gcttattttt taatttgtta aattcgtaat agcaaaatat
     9421 aatctaatat ttgttaaata attatccaaa gaaaatcaag taat
//
LOCUS       DAGSHT010000130         9463 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.134, whole genome shotgun sequence.
ACCESSION   DAGSHT010000130 DAGSHT010000000
VERSION     DAGSHT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.134"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..415
                     /locus_tag="MJB72_002650"
     CDS             <1..415
                     /locus_tag="MJB72_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBY8263734.1"
                     /translation="ENRITAVHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQII
                     TLNPYREDVYEFLVKEDGDFSKEIERLTEFLGYDIREYKASLMDIYINELMESDENDV
                     ENAKEKIEKYARYIGCSDENLYVARVNAIYTFANA"
     gene            772..2070
                     /locus_tag="MJB72_002651"
     CDS             772..2070
                     /locus_tag="MJB72_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBY8263735.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEIPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            2260..3651
                     /locus_tag="MJB72_002652"
     CDS             2260..3651
                     /locus_tag="MJB72_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBY8263736.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            3828..4415
                     /locus_tag="MJB72_002653"
     CDS             3828..4415
                     /locus_tag="MJB72_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8263737.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4448..5239
                     /locus_tag="MJB72_002654"
     CDS             4448..5239
                     /locus_tag="MJB72_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8263738.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNIDVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5420..7837)
                     /locus_tag="MJB72_002655"
     CDS             complement(5420..7837)
                     /locus_tag="MJB72_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263739.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(7850..8515)
                     /locus_tag="MJB72_002656"
     CDS             complement(7850..8515)
                     /locus_tag="MJB72_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263740.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(8597..>9463)
                     /locus_tag="MJB72_002657"
     CDS             complement(8597..>9463)
                     /locus_tag="MJB72_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263741.1"
                     /translation="LIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRI
                     NHRLIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTE
                     IQKDFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDA
                     EKKNIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKI
                     DIIDNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIG
                     KGSTFSIFLPNK"
BASE COUNT         3433 a         1161 c         1431 g         3438 t
ORIGIN      
        1 agaaaatcgt ataactgcgg tacattctta tattgatgaa gaatctataa aaaaatctaa
       61 tgccttattt aataattata aagatggttt tattgggaag gctgacgaat gcaaagtagt
      121 aaaacaaata ataactttaa atccatatag ggaagatgta tacgaatttt tggtaaaaga
      181 agatggagat tttagtaagg aaattgagag attgactgaa tttttaggct atgatataag
      241 agaatataaa gcgtcattaa tggatattta tataaatgag ttaatggaga gcgatgaaaa
      301 tgatgttgaa aatgcaaaag aaaagattga aaaatatgct agatatattg gatgtagtga
      361 tgagaattta tatgtagcaa gagttaatgc catatatact tttgcaaatg cataatttta
      421 ttaaacatta aatattagat gttcgattat ataaatttaa ataaaaaaat atccttatta
      481 ataacaaata gtttatatta aaaaccattc tttaaatgag tggtttttta ttttgaatat
      541 atatattcta aacctaatag cattcctatc tttaattagc caaaaatcaa ctggatttca
      601 tatagaccat aattttaaat tactaaaaaa gacaatatgt tgacaaagat gaaatatgtt
      661 gataaaatga aatagtcaat atggtgtctt aatttaaaaa tttatttatc ttgtattaga
      721 taaagctgtt ttatataaaa cataaataat tattataagg agaaatagat tatgaaagta
      781 aatgtataca atgtagaatc atttgtggat gaaagactta agattatggt ttcagactta
      841 attccaaata ctaaacttaa aataagtgtg aaaatggaat ttccatggtg taagggagaa
      901 gaattttcat cttatggagt gttttgttca aatgaaaaag gagaagtaga tttagattta
      961 gttgaaccta tagaaggtac ttataaggct gataattgta tggggttaat ttattcgctg
     1021 gaaaaatcta aaactgaagg taaaaatttt gcagaaaata tatctattga taaaccaata
     1081 attatgaata tgatatttga aacttctatt gaaagaaaag aagttgaatt aaagagaata
     1141 tttaaatcag aagatattac cacaaaatac atatctgatg agtttatagg aaaattattt
     1201 tataaacaaa attctaataa taaaacaatt ttaatgctag gtggttctga tggaaattta
     1261 gacgctctag accttttagc agctccactt gcatctagag gattcaatgt actaacagtt
     1321 gcatactttg ctttagaagg gttaccagat aaattagaag agattccatt agaatatttt
     1381 gaaaaagtat ttgagtggat tagtgaaaat gaaattacca gtactaaaga aatatttgta
     1441 catgggacat caaagggtgg agaattggcc tttttacttg cttcaagata tgaacaaatt
     1501 aaaaaggttg tagtatcaca acctcacatt tattgtttcc aagctcttaa tggattgatg
     1561 agtggaaatg atacttcatc atggtcatat aaaggagagc catttccata tatcaaagta
     1621 gacaatgata tattctttga agaacagaaa aaaaatatat caaaagatat tccatttgga
     1681 tttaatagta cttataagaa aagtcttaaa agagcagaga atagaaaaga ggctagaata
     1741 aaaatagaaa attccaaagc agatatactt atgattgctg gaaaagaaga taacatatgg
     1801 aatagttatg aagcttgttt agaggcatta gaaatctttg aagaaaataa ttatccatat
     1861 aacgtagaat tattaacata caataatatg ggtcatccgt taccaattcc atatattatg
     1921 cctctaaagg agactttatg tatgagtatg agtagaggga ttttttcttc aggtggaact
     1981 gtagaaggaa attcaaaagg acagtttgaa tcatggaata gaactattga attttataaa
     2041 aaacctttat caagtgattt aaataactaa tattaagtga tttaagtaac taaaaatgaa
     2101 gcggtctttc tatatatgca caaaatctat caattttata gatttaattt attgtttata
     2161 taatttgcat gttatatact aattttaatg acattgataa tttataaaca attataaagt
     2221 tgtaaactta taagagttat tgaaaggtgg agtgaagtta tggaaagact cgctagaagg
     2281 gaacaacctc atataaaatt tggaatgttt aagataagaa ttccatttat acattacaga
     2341 tttgagaaac cagaagctat tcaaggtata atatcaagta tgacaagttt aggcacaatt
     2401 ggccttagta cacaaatttt gggattaccc tatgaagtgg catggagtat ggctatcatt
     2461 aactcaatat tatactgttt gcatgttttt atgggagacc cagttgtacc tggctggatt
     2521 acagcttcta taactcttac aactgcatat ttgttaaagt tcagcatggg cattgaaaga
     2581 atacaagcat taacagcttt acaaatagat ttaggaataa tatttatttt aatgggtata
     2641 actggtgtag ctggaaagtt agttagtaag ataccaaatt ctattaaagg tggaatacta
     2701 atgggtgtcg gtatatcgac tataatatca gagtttaatc ctaaagggag atttgattct
     2761 tatcctatat ctataacagt aggaattttg gttgcttgtt ttgtaatgtt ttctgaaaga
     2821 tttgatactc taaaagttaa gaataaattt ttatttcatt taggagaata tggtgtagta
     2881 tcagctattt tagtgagttt aatagtaggt atatttagta aagaaataga aattcctaga
     2941 tttagttttg acaatttagt ttacatagta gattttaaaa atctgataaa tacagtatct
     3001 ccttttggaa taggttttcc aagtgtaatg ttatttatac aagggatacc aatggctttt
     3061 atgatataca taattgcctt tggtgatttt ataacaggtg aaaacttagt actttctgaa
     3121 agtgaaaata gaaaagatga atatatagat ttcaattcaa ataggtctaa tgttataagt
     3181 gggataagaa atatttttat ggcaatcttt tctccatata taccaatgtg cggtccatta
     3241 gctgcgacac ttacaggaag tgttgctcaa aggtacaagg ttggaaagga agccatgcag
     3301 tctgttttta gtggcatggg tacattagta tgggtttcag caatttttat ctgtttttat
     3361 ccaattgcgc agattgctac acctcttata ccattagcat tgtcagtaac acttttagtt
     3421 caaggatatt tatgtacaaa gctatcaatg gagctatgta atacaggtgt tgagaaggga
     3481 ttgattggac ttatgggagg tgtaattgct gctaaaggcg gaacatgggg acttgctgta
     3541 ggttttatac tgtatttcat tttaattgat tctaaaaaaa gaaaagaaaa agatattaat
     3601 tcaatagaag attgtgtata tgaaatatta gacaaaaaga agagtattta aaggaaaatc
     3661 tgagttgctg ttatctatta atagattaat tgcaactcat gttttattta taagttttag
     3721 gaattgcaaa aaaatgcaaa gctttttctt gacataattt cttgatatgt tactatagtt
     3781 atataacata tgtgttttat aaaaatttaa tggagaggtg aacatatatg ccacctaaag
     3841 taaagataac aaaagaaatg attttgaata cagtcttgga tattacaaga gaagtgggat
     3901 ttgaagctgt aaatgcgagg agtattgcaa gtaaattaca gtgttccact cgaccaattt
     3961 ttacttgtta cgaaaacatg aatgaattaa aaaatgaatt tcttgctttt gcatatgaat
     4021 attataatca gtatgttgtt aattatcgta attctaaaag tgtaagccct tatttactgc
     4081 ttccgctttc atacattgaa tttgctcaag aagaaacgta tttatttaag ttgttattta
     4141 taaatgattt agatttgatg atgaaagagg cgaatgattt ttataaagaa attgacaatg
     4201 aaaaaaaggc aagacttttt tcggaaagca ttggaataga gttggaatgt gcaaaagtaa
     4261 tatttttaga tttatttctt tatactcatg gcatagctgt cttaacagca acaaaaaaac
     4321 taacattaga tagatatagt gctgaaaaaa tgttgaaaaa tatattaaca gcttttataa
     4381 gacaagaaaa accagattgg aatttgtcta tttgatgttt attaactaat atagaagagg
     4441 tgaaattatg gaaacaaaac aatacttaat tgacttttat aatacttatg atgaagatag
     4501 tcgacttgca ttaaaacatg gaatggtgga atttctcact actatgcatt acattgataa
     4561 gtacataaaa tcaggtgatt gtgtgcttga aattggcgct gcaactggtc gttattctca
     4621 cacactggca cgtcagggat atgatgttga cgcagtggaa ttagtcgaac ataatataga
     4681 ggtttttcat aaaaatactc aatcaaatga aaatataagc attactcagg gtaatgctat
     4741 ggatttatct gttttcccag ataataaata tgatattacg ttactgttag gaccactata
     4801 tcatctttat aacaaagaag ataaacaaca agctttacat gaggcaattc gtgtaacaaa
     4861 acctggtggt gtggtttttg ctgcatatgt tatatctgat ggctgtctca ttgacgaggg
     4921 gttccataga ggaaatattg atgtatctga atatattgaa aaaggtttga ttgatccaca
     4981 gacatttgct gctaagtctg aaccaaagga tttgtttgag cttgtacgta aagaaaacat
     5041 tgatgatttg atgtctgcat ttaatgtaac tagactgcat tatgtcgcat cagatggttt
     5101 ggctttgtat atgcgtgaag cagtagactc tatggatgat gatgcatttg cattatattt
     5161 aaaatatcac cttgctactt gtgaacgtga agatttagta ggggttacaa gtcatgcaat
     5221 tgacatattt agaaaataag gaatatgctt gtttatgtct ctggtgatgt tgctcggaga
     5281 tataagtaag tgtatctgta taaaataaaa acaagtatag ggatagaaaa ttatgtaaag
     5341 tcaaaatttt ctatccctat agcttttaaa aaatcatatt aaaacttgta taaaaaatgg
     5401 ctatttttta tatacattct tactgagaac gtaagcgttc tgtaagtgat tttttttcta
     5461 tatttcttac aagtaattca gtcactaaaa cttgtatacc aattaatacg aatgaaaaaa
     5521 ttaaaatagt aaatatagaa aatttaaaat taataaagtc taatctaatt acatctttta
     5581 taaaagtgca aagtagattt gaaccaaaat aacctagtgt acttgaagta catatagctt
     5641 tgatacttat gtttagacct tctctataaa gcatatttcg cagttgcttt cttgtcattc
     5701 caacggcttg aagtaatgca aactcttttt ttctaattaa aacgctgttt attagggtat
     5761 tggtcatatt tagtatacca aagcaagaaa ttaaaccaac aaaaatgtat gaagctataa
     5821 ttatatattg aaatgctcta gtaatcattc tataatcatc ttcatatgaa taaagcatta
     5881 aatttgaatt tttctgaatt atacttaaca acttttcttc aacttcttta gaatgctttt
     5941 tatctattaa aatagaaaaa tctaaatttg tattatatgg tgttagagat tctaaagttt
     6001 tgtctgaagc aaaaaacata cctcctgtat tggaattgtc aatgattcct ataattttca
     6061 ttttcttttt aataatatca tttccactaa agatattaaa tgtaactgta tctccaactt
     6121 ttaaatttaa tccataatat tttgttctat atttatttat aattaaacta tctgaatcta
     6181 aatgtgtata gtctattttt ccactgacaa tatattcttc aagtgatttt gaaagttcag
     6241 gcgtaatacc tttaattaga gtaataaatt gtttttcgtc tctatctttg atgtaaggtt
     6301 ctataatatt acttttaatt gatttggatt caattaattt ttttactcca tgaatagatt
     6361 taatttcatt cttaagagaa tctgttagtg gattattttg ttggatttct gtaatatgct
     6421 catataagtt atcactctta atgctaatta tataatcttc gttatagaca aattgttgag
     6481 atagttgttt tttgaaatct attgaattag caagattagc gattgaaata aataaaactc
     6541 cactaatgct tagcgatatt aatgcaatca aatttttctt tctatttgac tcgatattct
     6601 ttaatgctag attttttata tttatataac taaaattttt tgcttttttt ctaaatttag
     6661 tttctgtata ttgcatggct tcaatagcag atattttaga aataattcta gctggtttaa
     6721 ataaagaaat tctaacagtt aaaaacatta ccagtgataa aattaaaatt ataaataaat
     6781 cagctatcat ttgccatgct aatggttgaa ttatgtaagc aattatacaa gaagctataa
     6841 cccctaatgg aataaaaata attgacaaaa tatttccttg tagagaaata atttttttta
     6901 actgaaaaga agtcatacct aacgatttca acttagcata catctgaact gaattaacaa
     6961 ctgaaatata aaaaatacta tatataacaa aaacgctaga aaaaagaaca actattccaa
     7021 ctactaaaac tgtaataatc tgctcaccat caattaaatt tgattcaata tagttatcat
     7081 ttaagtatat attatatttc ttaataccta aagataaacc aattttattt aactgtgtct
     7141 taattgaagc tgaatctttc tctgtatttt cttctgatag tttaactaat acatctacag
     7201 tatcaggtct tgactcgtca aaagtattac ttttattttg taaaggtgta gaagctatat
     7261 tcttaaaata atcatttgaa acgactgtat agtattgctt ggcattatct tgataataat
     7321 tttctagaaa tccaacaata ataaaatcac attgaacttc tttattcgta atatcatcat
     7381 aataatttaa tttaatgcta tctccaatat ctttatctaa accaaattcc tgtaaatatt
     7441 tttctgaaat agcaatttca tttttatttg taggtagttt gcctttcaat ggttgaaaat
     7501 tcataagttt aattgctgta tcatctgcat aaacaatcga cataatacaa tctgtctttt
     7561 tattacttcc aattaatttg taagttccta cgccactaaa gtttttatct ttatatagta
     7621 tattttttgc tttttcatct acagatttat atatagcatg atatggagat gcattaacta
     7681 aatctacaga ggcattatat gaaactaagg tcactgttaa taataaacat gtagaaatca
     7741 ttataatgat acctgaaaga tatgttttaa gtttattaat ttttaaatca tttaaagcta
     7801 tttttcttat aaatgtgcga ttattatttc taaacatata ctacacctcc tagttaacaa
     7861 tttttccatc ttcaatacga ataattctat cagttagttg tgcaatttca ttattgtgtg
     7921 taatcattac aattgtttgg ttgaattttt tacttgtcac tttaagtaaa ctcagtacat
     7981 ctgaactagt tctactatca aggttacctg ttggttcatc agctagtata atagctggtt
     8041 ttgttacaag tgctctagct atagcaacac gttgctgttg accaccagaa agattacttg
     8101 gtatatcata taatttttct tcaagagaaa gcatatttat aatttcattt ataaaatcct
     8161 tatctatggt ctctccatcc aatcctattg gtaagataat attttcatat acattaagta
     8221 aaggaacaag attataattt tgaaatataa aacctatatt tctgcgtcta aagattgtaa
     8281 gctgttcatc agacatagta gataattcct taccctttat aataaccttt ccagatgtag
     8341 gattatctaa tcctcccatc atatttaata atgtggattt gccactacca gatgtaccta
     8401 caatagaaat aaattcgcct ttttcaatag atagagttac accatcaaga gcttttgtaa
     8461 tgtttgaact ttgtccataa tatttcttta aattaattac ttgtaaaata ctcataataa
     8521 tacctcctta acatcttgta aatacagtat aaaaccaaaa tgttgcagaa tcattacaaa
     8581 tgaaatattt aaaaatttat ttattaggta agaaaataga aaaagtcgac ccttttccaa
     8641 tatctgatgt aactttaata tagccacctt gaagagatat aatttttctt gaaagataga
     8701 gacctattcc aataccttct atgtcatgga catcttcttc acgataaaat cttttaaaga
     8761 tttctgcttg gcgactttct gatattcctt ttccattatc aataatatct atttttgtat
     8821 acataaccca acattctgta gctacacgta tcttaccatt agaacttgtg tattttacag
     8881 cattatctaa tatattaaaa atagcttcag aagtccattt cttgtcatga attatatata
     8941 aatttgggtc acaatcaact gtaacttcaa tattcttttt ttcagcatca aacaaaattc
     9001 cactaagtgc tgtagcaatt gtttcataaa ttgaattttt ctttttagat agtgtgatta
     9061 tacctgtctc tagtctggag gtttttacca tagattgcat taaaaaatct aatttatcta
     9121 attggttttc catatccatt aaaaaatcct tttgaatttc tgttttaaat gtttgattta
     9181 gtagagtagt attaatcatt tttaaatttg caataggagt tttaatttga tgagatatat
     9241 cagaaactag ttcttgtaaa tcagctttct ctatagcaat actattgcga ttttcttgaa
     9301 taacttcata cattctaatt aatctgtgat tgatacgtga taaaatagtt tcttcttcta
     9361 attcaatttc aatattatct tcactattca tcatactatc taaagtgtga catattctag
     9421 aacaaaatat aactattttt ttatgaagta aaaatattat taa
//
LOCUS       DAGSHT010000131         9448 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGSHT010000131 DAGSHT010000000
VERSION     DAGSHT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.135"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            436..1278
                     /locus_tag="MJB72_002658"
     CDS             436..1278
                     /locus_tag="MJB72_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8263742.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            1423..3351
                     /locus_tag="MJB72_002659"
     CDS             1423..3351
                     /locus_tag="MJB72_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8263743.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIV
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            3412..4839
                     /gene="ascB"
                     /locus_tag="MJB72_002660"
     CDS             3412..4839
                     /gene="ascB"
                     /locus_tag="MJB72_002660"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBY8263744.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            5250..6026
                     /locus_tag="MJB72_002661"
     CDS             5250..6026
                     /locus_tag="MJB72_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY8263745.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            6042..6407
                     /locus_tag="MJB72_002662"
     CDS             6042..6407
                     /locus_tag="MJB72_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY8263746.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(6583..7332)
                     /locus_tag="MJB72_002663"
     CDS             complement(6583..7332)
                     /locus_tag="MJB72_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY8263747.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            7530..7682
                     /locus_tag="MJB72_002664"
     CDS             7530..7682
                     /locus_tag="MJB72_002664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263748.1"
                     /translation="MRRDNNLLMKDYKYKGYFEGVEIFDNVNDLNLNTLTTVSRFVFV
                     FLFTIT"
     gene            7810..8235
                     /locus_tag="MJB72_002665"
     CDS             7810..8235
                     /locus_tag="MJB72_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263749.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            8267..>9448
                     /locus_tag="MJB72_002666"
     CDS             8267..>9448
                     /locus_tag="MJB72_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBY8263750.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMTVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VD"
BASE COUNT         3630 a         1015 c         1665 g         3138 t
ORIGIN      
        1 aacctattgt gagagcttaa attactatag aaggtcgttt tttattacag aaataaataa
       61 gagatagaga atagtttaga ggtgagaaag aaaatataat ttaaaacata taacaacata
      121 taatcttcta ataaataaag gatttaatgt tgttgatttt aaaaattata ataaaactat
      181 taaaaatgga ctctattaaa ttaatattaa taagaataat tattataact gacaaaaagc
      241 aacataaaag attgatgaag tatatggaaa taaaaagttg acaaaatatg tctgatgttt
      301 tatcatatta attggaatta ggtagtaatt gtttaagtac aagctaagct tattcattaa
      361 taatagcagt atcctaggaa ggagtatgcc cattattatg gataagcttt ttttatgctt
      421 aaaaggagga taatcatgat aattaataag atattaaaca ataatgttgt aatcacatta
      481 gatgataatg atgaagaagt tatcgttatg ggtaaaggaa ttggttatca aaaatcaaaa
      541 ggcaatctaa tagataaaac taaagtaaat aaagtattta gaatatcaaa taaagaaata
      601 tcaaacaaac tacaagaatt actcaataat attcctatag aacacatgaa attatcaagt
      661 gaaattatag aatacgcaca aataaagtta aacaagaagc taaatgaaag tatttacata
      721 tctttaagtg accatacata ttctgcaatt caaagaatga aagaaggaat aaatgtaaaa
      781 aatgcaatcc tttgggaaat taaaagattt tataaagaag agtttgaaat tggaatgaaa
      841 gcactagata ttattgaaaa caaaacaggt ataaaattac cagaagatga ggctggattt
      901 atagcctttc atatagtgaa tgctcagtta agtgaaggtc atactttggc atctgatatt
      961 acgaagttaa tacaagaagt acttagcata gttaggtatc attttaggat agaatttcat
     1021 gaagaatcag tgttttatta tagatttata atgcatttaa agttttttgc gcaaagattg
     1081 cttttagata gtgcacatga aggtgagaca gacaaagaat tactaagcat tattaaatct
     1141 aaatataata aagagtttga atgtgtagtt aaaattaaaa atttcattaa aaaacaatat
     1201 aactacatat taacagatga tgagcttata tatttaacaa tacatttagc taaagtagtt
     1261 aaagactcaa atatgtaagt gaaataattt ttcagtagta tattaagaat gattgaaagg
     1321 agtgatacta acaaagttat acttatggtt ggatggtaac attgagttgg ccaaaccttc
     1381 aaatttcaaa aaattaattt aaacgaaata tggaggagaa aaatgggaaa atacaaacaa
     1441 ttagcacaag agatagtaaa caatgttggt ggaaaagaaa atattagtgg gttagtacac
     1501 tgtattacaa gattacgttt tactttaaaa gatgaaagta tagcaaatga caatgtatta
     1561 aaaaacatgg aaggtattgt tactgtaatg aaaagtggag gacaatatca agtagttata
     1621 ggaaatcatg ttgaagctgt ctataaagat gtagttgaaa taattggttt agatgattca
     1681 agtacaagtt cagaaactaa aaaatcaggt aatatactag ataaaggtat agatataata
     1741 tcaggaattt ttcagccagt gttaggaata atggcagctt gtggtatgtt gaaaggattt
     1801 aatgcattat ttgtagcaat gggattatat agtgatgtat ctggtggtta tatggtaata
     1861 aatgcagcag gagatgcatt atttacattc cttcctttgt tcttaggata tacttcagct
     1921 aagaagtttg gattaaaacc aatgttggga ttagcactag gagctgcagt atgttatcct
     1981 gctatacaag gtagcagtat atcagcagga gcagatgtga tgtacacttt atttaaagga
     2041 acaatgtttg catcaccagt atatattgat ttctttggac taccaattgt atcaattgat
     2101 tatactggaa cagtcgtacc aattatattt atagtgtatt ttgcttctag gtgtgaaaaa
     2161 ttatttaata agtgtgttcc aaatttagtt aagtttttct ttgtgccaat gcttacatta
     2221 ttaataacag tgcctgttgc acttatagtt ataggcccta tagcaacatt tggttctaca
     2281 cttatttcac aaattgtaat ttcaataaga gattttagtc cactattggc aggtgctata
     2341 gtaggattta cttggcaaat tcttgttatt tttggtatgc actggggatt tattcctgta
     2401 tatataaaca atgttatgac tttaggatat gataatgtta tgatgccatt ctttgcctgt
     2461 acatttgcaa gttcggcagt tgtgttggca atatttttca agactaaaga taaaaaacta
     2521 aaagagatgg caataccaaa ctttatatct ggtatatttg gagttacaga gccagcaatt
     2581 tatggaatat tattgccttt aaagaaacca tttataataa gttgtattgc tagtggtatt
     2641 ggaggcgctt tctatggatt ctttaatctt agaaaattta taactggagg aatgggaata
     2701 tttgaacttc cagcaatgat tgaaccagat gggggaatgg gtaacttaat agtagctctt
     2761 tctggtatag ttatatcaat ggtcgtagct tttgtattaa ctatgatttt atataaagat
     2821 gagaaagtag aagagtctaa aaaagtaaga aataagggta aagaagaaat aaaagaagtc
     2881 aagtctacta aattagaaag agaaatagta acaagtccta taaaaggtga agtattaaaa
     2941 ttaagtgata tagaggatgc agcatttgca tctggtgtac taggtcaagg ggtggcaata
     3001 atacctagtg atggaaaagt agttgctcca gtagatgggg tagttacaac cttatttcct
     3061 agtctacatg ctatagggat tctttctgat acaggtgttg aagtgcttat tcatattgga
     3121 ttaaatacta ttcaattaga gggaagaggt tttgaggcat gtataaaaca aggtgataga
     3181 attacaaaag gtcaaactat attaaacttt gatgttgatg caataaaaga gttggggtat
     3241 agtactgtaa ctccaattgt tattacaaat tcatcacaat ttttagatgt agtagaaaca
     3301 gaaagtaaaa atattgaatt agaagacaat ctaattacag tcttatttta gtaaagattg
     3361 aggtattagc ataatctagt gctttggtta taattttagg aggctttgac tatgagtttt
     3421 aagaatgatt ttttatgggg aggagctact gcggccaatc aatgtgaagg tggatacaat
     3481 gaagacgaaa gaggattggc aaatgtagat gtatgtccaa caggaaaaga tagaacagca
     3541 gttattacag ggaaactaaa aatgtttgat tttgatgatg agcattatta tccagctaaa
     3601 actggtattg atatgtatca taattataaa gaagatataa agttatttgc tgaaatggga
     3661 tttaaagtat atagaatgag cattgcatgg agtcgaatat ttccaaaagg tgatgaagaa
     3721 acaccaaatg aaaagggact tcaattttat gaaaatatat ttaaggagtg taaaaaatat
     3781 aaaatagagc ctttagtcac tattacacac tttgattgtc caatgcattt agttaaaaag
     3841 tatggtgcat ggcgtaatcg aaaaatgata ggtttctatg agaaattatg caatgttatc
     3901 tttagaagat ataaaggatt agtgaagtac tggctaactt tcaatgaaat aaatatgatt
     3961 ttacatgctc catttatggg tgctggtatt tgctttgaag aaggtgaaga tgttgaaaag
     4021 ataaaatatc aagctgccca tcatgagctt gtagctagtg caattgcaac taaaatagca
     4081 catgaagttg attcaaataa cttaattgga tgtatgtttg cagctggaag tgtatatcct
     4141 tatagttgta atcctaatga tgtatgggaa gctacaaagt tagatagaga aaattacttc
     4201 tttgtggatg tacaatctaa aggtaaatat cctaactatg cgttaaagta tatggagcaa
     4261 aaaggtatta caccagaaat ggaacctggt gatattgaat tattgaataa atatacagta
     4321 gattttattt cattttcata ttacaatagc cgttgtgtta gaaccgatga aaatgcagat
     4381 gatatggctg aaggaaacat atttacatct gctaaaaatc catatctttc atacagtcaa
     4441 tggggatggc caattgaccc attaggtttg agaattacac taaatcatgt atatgataga
     4501 tatgaaaagc cactatttgt tgtagaaaat ggattaggtg ctaaagatat agctgatgaa
     4561 aatggttatg tagaagatga ttacagaatt gattatataa gagaacatat aaaagctatg
     4621 catgatgcag tttctataga tggggtcgat ttattaggtt atactacatg ggcacctatt
     4681 gatttagtaa gtgctggtac aggtgaaatg gaaaagcgtt atggatttat atatgtagat
     4741 agagacaata gtggtaatgg aagtctaaga agaatgaaaa agaaaagttt tgaatggtat
     4801 aagaaagtaa ttgcttcaaa tggtgaagat ttatattaga caagaaatct atgttaaata
     4861 agaaatttgt attaaataag aagagtataa aaattaataa taagtataat ttttaacttt
     4921 tttaactata gaaaatcaat acagaattat tttacattgt atttatgcaa tagtatgatt
     4981 cacaaataaa attttagaaa ataaaattgt taagaagtat gggaagtaag tgcaatttac
     5041 tttgtaataa aagatgaaaa aggcaagctt aggttttaag cttgtctttt ttattatgta
     5101 taaactatga tactttaaaa ttctatgaaa aatttattta atagagaaat taaaaattta
     5161 atggtaatca aattctttac caatgaatat aaaaaatgtc tacgtgttat aatagtttaa
     5221 actaataaac gaaaggtaga tgaatgaata tgaaatacca acatataata gaagttgaca
     5281 aagaactttg cattggatgt gggctttgta aaaatgattg tccagtaaac aatataatta
     5341 tagaaaataa aaagtcagta attaaaaaac aagattgtct aatgtgtgga cattgtgcag
     5401 caatatgtcc cacaaaagct attaccttga caggatttga tgaacctcct atagaactta
     5461 caaataagcc aaagctagat tcagatgaat tactaatggc aattaaatct agaagaagta
     5521 tacgtaaatt taaagacaaa gaagtttctt cagaaattat aaagcaaatt attgaggcag
     5581 gtaggtatac ccctagtgct aaaaattcac aagatgtatc atatattgtt ttagacaata
     5641 aaaagtcaat ttatgaaaac gaagcagtga aattttttag aaaaataaaa cctattgcaa
     5701 atatagctat taaatattca aaagaagttg agattgacga taactttttc tttaagcatg
     5761 ctcctattgc aataatgata attacaaaag ataagattag tggttcactt gcagcatcaa
     5821 atatggcatt aatggcagag tcttatggcc taggtgtttt atttagtgga tatttttcag
     5881 atgtagcaaa taattcgcca aaattaaaaa agcttttagg tctaaaacgc agtaatcatg
     5941 ttcttacaac attagtaatt ggatacccag atgttaaata tagacgtact gcacagaaag
     6001 aagtagcaac tgttagatac ttataagaga gggtggattt tatggaaaaa gaatgtcaac
     6061 aaagaatgca agaaataacg tctaaattta aagaatgtcg caaagctatc tctgctatgg
     6121 gtgatgagac tagacaattg attttaattg ctctcttaga gagtgatttt aatggtatac
     6181 gagtgggaga aatcacaaaa aaaacacatc tttctcgtcc tgctgtatct catcatctta
     6241 agatattaaa agaagcagag attgttaatt tgcgtagaga aggaacaaaa aattattatt
     6301 atcttgattc aaaaaaatca caatggaggt cactaacaga gctaataaat cttatatttg
     6361 ttggaataca acatattagt gaaaatgata taagaaaagg tgattaaatt atacttgatt
     6421 taatacaaaa agattgattt aggaactaaa aagaactaaa aagttcactt tgagacacta
     6481 tcttttgaac tatacccatc ttaggagaca gttaaaatat gtaaaactgt ataaaacaat
     6541 gggtatagtt cattttataa tggtttaaat attaagaata tattattcta aatagtatct
     6601 ttctctgttt tgagttttat ttttatactc aatagcttca gtggggcaac cacaaataca
     6661 tgccatacaa tgagtacagt tatttttcca aactggtttt ttgtttttta aattaatatt
     6721 atttaaagga catagctcta cacattttcc acagccaatg catttatcag tataatgaaa
     6781 tcctttggca ctaataattg tcttataaaa aactgtattt attataccac ttttaattgt
     6841 acctcctaag ccactaggag taataatttc aaattcattt tcatttcgta tatcagaaat
     6901 aatcctatat atttgtttat ctgcttcttt aatcatttgt tttgatgttt ctttgtctgt
     6961 tgtagaaaat aaagctatat aattctctgg cattttaatt tctgccatac cttttaattg
     7021 ccattccttg tatttacata gtttttgaat atagtttatg gattttgctg tatctccacc
     7081 acaagtcatt acaaagtaaa ctctgttgct tcctagaaat tttgtttctt ttataaaatc
     7141 cgtaaccact attggcaatc tccatgcata agtaggacat acaaatatga atggtttatc
     7201 tgttgatatt aattcatctg ttttttcctc ttttataagt tgatttagtg atatcagttc
     7261 atcatttaat tcttgtgcaa ttttacgagc cacatatcgg ctattaccag ttccgctaaa
     7321 atataaaatc ataataatat ctccctaact attttttatt ttaactctta attttttatt
     7381 tattatagta atttattgat atattctatt caaatgtatg taaaatttag tagttaagaa
     7441 aattataaca cattagtaat ttttatactt attggtaaag aatttgatta ccattaaatt
     7501 tagcaacaag acatcaactg gacttgtata tgagaaggga taataatttg cttatgaaag
     7561 attataaata taaaggttat tttgaaggtg tagaaatatt tgataatgta aatgacctta
     7621 acttaaatac tttaacaaca gttagtagat ttgtgtttgt ttttttgttt acgataactt
     7681 aaggaaatat gttacctgaa caataaattt atataataaa agtatataaa tatatatatg
     7741 aatgttaaga ttagttgata gaatttaaat aataaatcca atattataaa cataggaggt
     7801 atgaaaaata tgattaatga gaatttaaaa agtctaagaa aaatcaatca gtatacacaa
     7861 gaagaattag ctgaaaaact taatgtttct agacaatcaa tagcaaaatg ggaaagtgga
     7921 gaatctattc ctgatatagg tagttgcatt aaacttgcaa aattgtacaa tgttaaacta
     7981 gatgacttag ttaatcattc ggaagaaaaa acaggtatta tagtacctcc taaaggaaag
     8041 tttttctttg gagcagttgt agttggtgaa cgtggtcaga ttgtcattcc aaaagaagca
     8101 agagaagtat ttaatataaa tgcaggagat aaattattgg tacttggaga tgaggaaaga
     8161 ggattaggaa ttgttcatca acgtgatttg ataaatttta taggtcatgt gggagtagta
     8221 cataaagaag aatgatttaa aaaaattata aaaggaggta tgaaatatga gtaaaatagt
     8281 attttttagc attcctgcat atggtcatac taatcctact attgaagtag ttcgtgaatt
     8341 agtggataga ggaaatgagg tattgtatta ttcttttaat gaatttaaag ataaaattga
     8401 aggtgctgga gcaaaattta tttgttgtga taaatacctt ccagaattac tccctggaga
     8461 tgagaaaaaa attggtaagg attttcctgg attaattgag atgacagtag atactactat
     8521 ttctttagat gaaaaagttt gtagagaact taaagatttt aatccagatt gcattgtatc
     8581 agattcatta agtttttggg gaaagttatt tgcacaaaaa cttaatattc catatgtatg
     8641 ctctacaaca acttttgcat ttaataaaca tacagcaaag ttgatgaaac aaaactttat
     8701 agaaattata cgtatgattt ttggtgtaag acgcattaat aaaaaaataa agcttttaca
     8761 aagaaaaggg tatgaagtaa aaaattttat ttctatagtt tctaatgata atgatacgga
     8821 taccattgta tatacatcaa aagaatttca accaatggta gaaaccttct ctagtaagta
     8881 ttcatttgta ggtccatcag tatcaaagtt gattattgaa cctaaagaga gaaaaagaaa
     8941 gctaatatat atttcgcttg gtacaattaa taataaaaat gttagctttt ataaaaactg
     9001 tataaatgca tttaaagatt ctgatgttga tgtgattatg tctgttggta gaagtactga
     9061 tattaaaagt ttaggaaata taccaaataa ttttgaggtt aaaaatagtg ttgaacagat
     9121 tgctatactt caaaaaactg atgtgtttgt tactcattct gggatgaata gtgttaatga
     9181 aagtttatat tatggagtac ctatggtatt atttcctcaa cattcggagc agagaatggt
     9241 agctaagaga gttgttcatt tgggagcagg aattatgtta aaagaggata aatcagaaag
     9301 tattaaaaaa gcagtatttc aagttataaa tgacaatgaa tataaagaaa atgcaactaa
     9361 actatcaaaa agcttttata atgcaggagg ctcaaaaaaa gcagcagatg taatattaca
     9421 gattatatgt aatagtagac aagttgac
//
LOCUS       DAGSHT010000132         9315 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGSHT010000132 DAGSHT010000000
VERSION     DAGSHT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.136"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            270..1577
                     /locus_tag="MJB72_002667"
     CDS             270..1577
                     /locus_tag="MJB72_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263751.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1663..1925
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2051..2854
                     /locus_tag="MJB72_002668"
     CDS             2051..2854
                     /locus_tag="MJB72_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY8263752.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2892..3503
                     /locus_tag="MJB72_002669"
     CDS             2892..3503
                     /locus_tag="MJB72_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY8263753.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3535..4206
                     /locus_tag="MJB72_002670"
     CDS             3535..4206
                     /locus_tag="MJB72_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY8263754.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4505..4867
                     /locus_tag="MJB72_002671"
     CDS             4505..4867
                     /locus_tag="MJB72_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBY8263755.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5303..6004
                     /locus_tag="MJB72_002672"
     CDS             5303..6004
                     /locus_tag="MJB72_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBY8263756.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6275..7093
                     /gene="larE"
                     /locus_tag="MJB72_002673"
     CDS             6275..7093
                     /gene="larE"
                     /locus_tag="MJB72_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBY8263757.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7148..8296
                     /locus_tag="MJB72_002674"
     CDS             7148..8296
                     /locus_tag="MJB72_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBY8263758.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8432..>9315
                     /locus_tag="MJB72_002675"
     CDS             8432..>9315
                     /locus_tag="MJB72_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263759.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKS"
BASE COUNT         3762 a          838 c         1651 g         3064 t
ORIGIN      
        1 taatttgata atatgtaatt ataacattat gatataatta caataaagta tgtaataatc
       61 tgtagaaatt tcaagctagt tatctaagtt ttcggataac taaatctaaa cggaatacat
      121 aatgtattaa ataaatgatg atactaaaat gatactattt ttatagaaat ttattgaggt
      181 atataattaa tgaaaataat atattaaatt atataaatta aattatataa tatattgagt
      241 tttacataaa agcaaaggtg ggaataaaaa tgaaagaaat tgctgatgct ataacatcag
      301 agtatttaag ctatggtatc aatgaagata ttagtatcta tgaaggtaga ttttgcatat
      361 atttagataa aaaatataga tgtaatggga aaatttatta taaaatgaca cctccaatat
      421 ctataagttt taaagcagac ataggttgtg ttgaagagat agacaatgaa gatgacaatt
      481 tagctttaga ttatgataat gctattcttg aagttcatgg atataaaatt atttctataa
      541 caataaatac tttaagtgaa tttagtgtag aaggttatat caatgatgac tgcataaaat
      601 ctaagaactc ctatgttgaa tatgtagatt ttaatataat aaacttagat aaaatacctg
      661 gaaaactcat taaatacaat gataaggtat acgcaggtag aattgaattt gatataaatg
      721 attatgttgt aactatagac aagcgatatg attatagaaa agaattaaaa tcagaattaa
      781 agtctaaatc tggagcaata ataactcata taggcaggat tagaagaaaa gatggtagaa
      841 tttttagaac aaataataca ataaatcttt tagataggat atcaacagct ttgagtttta
      901 tgtgtggaag atatgtaggg ttttgtttgg ccaaagggta tagaagtggt aatgaagttt
      961 atagaatatg gaatgaaaat caaatatctc cttttagata tgtaccaaca tggtcagata
     1021 cactttcaaa ttatcataat atggaaaaat atataagcct tatgtgtaaa aagctagaag
     1081 atttttatta tggttctgct ataaagagtg ttgttgattg gtacatagaa tctcttggaa
     1141 gtgctaccat ggaaaacaat ataatatctg tacaaattgc acttgaaact ttgtcatatg
     1201 taattctagt ggaacaaaat aagatactta cagatgaagc atttgactgt aatttggcat
     1261 ctaaaaatat aagactctta cttgatacat gtaagatacc atatggtaaa catgaattaa
     1321 atatatttga taatataata aaaaataaat ttgatgatgg agtggattta gttatttatt
     1381 taagaaatag catagttcat ccaagtagaa agactcatag agctgtgtta gaggttgaag
     1441 atatttggaa tataatttca ataggaacga gatatattga acttgtttta ctctttattt
     1501 taggttatag aggagaatat tcaaacagat tagtagaaag atgttatggt gaagtggaag
     1561 ttgtaccttg gaactagatt tttatattta ttttaaaata tatattgaca tatattgcct
     1621 tatgctataa aatcaatatc ataataataa ttcagaataa cattaaatgc taagaaaaga
     1681 aatagtaaaa gtagttaatc ttaaacagag agtttccgat tggtgagagg agcaatagat
     1741 tttactttga acacatcttt gagcaggact ctgaaagagt agaaatactt tattaggaga
     1801 tttccggaat ccttgcaccc gttatagtgc tagagtataa taattttatt gtacttaaag
     1861 aggttagtgt cgtgaggcat taatgaacag aggtggaacc acgagagtta actcccgtcc
     1921 tctagcagaa gctagaggcg ggagtttttt aatataattt tttaagcgcg cagagaatta
     1981 tattgcaata gatacacttt ttaaaaaata atcaaaatat tttaaaaatt aaaagacgag
     2041 ggagaatatt atgaaaaata tattaaaaaa agtaggaata tttacaatta tgttaggttt
     2101 attaggtgga gttgtaggtt gttcaaaacc agataatgaa aaagataagg atgcttcaaa
     2161 ggaatctaaa aaagaagtag ttgtagggtt tgataatacg tttgtaccaa tgggattttt
     2221 agatgaaaag ggagatacag taggctttga cgtagactta gctaaagaaa catttaaaag
     2281 acttggaatg gaagttaagt ttcagcctat agattggtca atgaaggaaa ctgaattaaa
     2341 tgattcaaaa actgtagatg tattatggaa tggatattct ataacagacg aaagaaagaa
     2401 aatagtatca tatacagaac catatttaca aaataaacag ataatagtta ctctaagtga
     2461 ctcaaaaata aattcaaaag ctgatttaaa agataaagaa gttggaacac aacaaggttc
     2521 aacagcactt gatgcagtgg aaaaggataa agattttatg aatagcttaa aaggtggagc
     2581 accagttctt tatgatactt atgataaagc acttagagat ttagagatag gtagaactag
     2641 tgcagttgtt ggtgatgaag ttttaattag atactatatg ggacaaaagg gtgaagacaa
     2701 atacaaggta ttaaaagacg attttggatt agaagattat gttgtagcaa cttctaaaga
     2761 aaatccagaa ctttgtgaga aaataaatga aacattaaaa gaaatgaaaa aagatggaac
     2821 ttttgacaag atttatgata agtggtttaa atagaagata agttagctgt taagaagggg
     2881 gcactaaagg catgttacaa ggtttagaga tagttatagc aatgttttgt ataacattga
     2941 tagtatcaat tccattgggg attggagttg catttttaag attatcaaag aataaattag
     3001 taagtggtat tacacaatgt tatatattga taatgagagg tacaccttta cttttgcaaa
     3061 tgatagtcat attttacgga ttacctcttt taggaattgt atttgacaga tttacagctg
     3121 gagtagtagc tttcttctta aattatgcag catattttgc agaaatattt agaggtggaa
     3181 tacagtcaat cgatagagga caatatgaag cttcaaaagt actaggattt gataagttta
     3241 ctatgtataa gagagtaatc tttccacaag tttttaaaag aatactagca cctatttcaa
     3301 atgaagtaat cacattagta aaagatacat ctttagtata tatattagga ttaaatgata
     3361 ttttaagaat atctcaaata gcaatgaaca gggaagcaag tctgttacca ttatttgagg
     3421 caggagcgat atatcttata tttgtagcta tacttactaa gggatttgaa ttacttgaaa
     3481 aaaaatattc ttattataga taaagttaca atattatagg aggaggtttt ttatatgttg
     3541 aaaatcaaaa acttaaataa atcatttaaa aaaaatagag tacttaaaga tatttctttt
     3601 gagttagaag aaggtcagat tggagtttta cttggtaagt ctggtgcagg aaagaccaca
     3661 atattaaggt gtataaatgg tcttgaagaa tttgatagtg gagagattat aatagataat
     3721 gaggtaataa aaaataagag agatatggca aaaatacgag gaaaaatagg tatggttttt
     3781 caaaatttca acttatttcc tcatatgaca gtccttgaaa atataattga atccccagtt
     3841 aatgttttta aagttcctag aaaagaggct gaagagaggg ctagagaact tttaagatta
     3901 gttgatttag aagataaatt aaattcttat ccatttgaat tgtcaggagg acagcaacag
     3961 agagttgcta tagctaggtc atgtgcattg atgccaaaag tcctttgttt tgatgaacct
     4021 acttctgcac ttgatattga tactattcaa agagtagtca atataatgaa cagacttaaa
     4081 gacaaaggta tgacaatatt aataattaca catgatgttg tcttctcaaa taatgttgca
     4141 gataaaatta taagtataaa agatggtata gtagaaaatg ttcaaataaa agaaaaaatt
     4201 gtttagagtt ttataaatat aaactagggt agaattttat aatataaata aagggcaagt
     4261 aaaatcaaat atactatgcc cttttgttat ctcaaaattt aatcttatat tttatgttta
     4321 agttacatta taatttataa taaaaataaa aggactattt aaatatataa tgtgaataaa
     4381 tgtttaataa tttgaaatat gataattttt tatgttgaaa gcttataatg attatgatat
     4441 aaattggtta tagtatacaa tttatgggta taacaataaa aaattagaaa ggaattttta
     4501 taaaatgatt aaattatatg gatatacaaa atgctctact gttaaaaaag ctaaaaattg
     4561 gcttaaggaa aataatttag aatttgaaga tatagatatg gttcaaaacc ctcctagtaa
     4621 agaggagtta aaatcaattt ataagactag tggatatgat ataaagaaat tttttaatac
     4681 aagtggtatg aaatatagag aattgggtct taaagatatt gtaaaaactg aatctgatga
     4741 taaactttta gaaatattgg taagtgatgg tatgcttatt aaaagaccat tacttttgga
     4801 tggtaaaaat gtattattgg gctttaaaga agatgtatgg aaaagtactt tgctaaaaga
     4861 agattaggaa gagataggtg tgttgattaa aattattaat taatacacct ataaatttat
     4921 gtattaaatc taatttttat aaatattaat tagcaaattc aaatcaaaaa aattcatatc
     4981 aacaaacata ttacttctta ttacaaatta tatcatatgt tttatattaa aatattataa
     5041 tattacaacg aattgttaca aaattactta ttattaacca tgaacatttg ttttgtgcta
     5101 tcatatttat ataagggaat tgtcataaaa aatatcttgt tcatacagtt ttatatagaa
     5161 aaaagaaatg aatcttttct ttatataagt atattttgta aattgcctct atatttatag
     5221 tttaaataat agtggtatta aaaatctgac aacaaaaata aaactttcaa aaaattaaat
     5281 ctaaaaagaa taggagataa atatgagtat tgaagattta tatgattttg aatgtgttcc
     5341 tttgaacaat gagattgata ttgatgtttt atttgaaaag atgatagaag atggaaaaga
     5401 aaaagaatat acaccaatta tagtcattga tgaaaaagta gggctattaa gaaaaaatat
     5461 agatatgatt actaaagagt atggttctct tgaaaactat agagagtatt gtttaacaaa
     5521 atataatgaa attgatgtaa atcaattttt tgattttagg aagagtgaga tttcagaatt
     5581 attaaaggag ctaattgatt tagaagatga tttttatgat tataaacatt ttagaccaat
     5641 tatgaatata gatatatttc atgaatataa caaagtttac atagctaaaa ttccaacaat
     5701 taaaccatat gaagtatttg catacatacc tattggcgga tttaatgatt gtcctagaga
     5761 tgaagaacat atagctattg caaaatattg gtatgaaaag catggaatct ttccaatcgc
     5821 aattggatgt gatacagttc aatattctgt aaaaaattta actaaagatg gtaaaaaatt
     5881 tgatgaatta tgtattgaat tagtatttta ttgtgaggat attattatac aagggtatga
     5941 aaccttaaag gctcttaagg atgtattaaa acgctctaca atagtgcttt tttggtggga
     6001 ttgatattcc atcatttaga tagtattaaa ttgttagtca ccaagaatcc acttgcttta
     6061 gttatgggga gtgtgtcaaa ctaggttttt atacacagtg tagcatagta atatgtttaa
     6121 attacaaagg ttaaaaaaat actttaatat ttttaatatt ataattaata gatttaaagg
     6181 taattataat ttataaaata aatactttat atgttataat cattaagaat gtaaaaaaat
     6241 aatatatagt tttatagttc atatttttcc agatatggag gtaaattttg tgaaggaaaa
     6301 agaaaaatta gataaattaa aaaaaatgtt acttgaatta ggcagtgttg tagttgctta
     6361 ttctggtgga gtagatagta attttttgct taaggtcgca aaagatacat taggtgaaaa
     6421 tgttatagct gtgactattc atgcaatgat gcattcaagt agggaaattg aagaggctaa
     6481 acaatatact caaaattttg gtgttaaaca tatcatatta aatatagaaa actttgattt
     6541 aaaagaattt aaagaaaatg gtgttgatag atgttatcat tgtaaaaaat atatattctc
     6601 taaaataaaa gaggtagcaa aagaacataa tataaagtac atagtagatg gaacaaatat
     6661 agatgattta ggagactata gacctgggct taaggcattg agtgaattag gagtaattag
     6721 tccattaaaa gatagtagtt taaaaaaaga agaaattagg tcgctttcaa aaacattagg
     6781 attaaatact tttaataaac catcatttgc atgtcttgcg agtaggatac cttatggagt
     6841 tgaaataaca gatgaaaatt taagaatagt agaaaagagt gaagaatatc tatccaattt
     6901 aggtttttct caatttagag tgagaatgca tggagatata gctagaatag aagttggaca
     6961 ggaagaacta ggtaaatttt ttgaaaataa caattttaat aaagttgata ctaaattaaa
     7021 aatatttggc tttaaatatg ttacactaga tatgtcagga tataaaatgg gaagtatgaa
     7081 tttaaatgtg tagattattt taaaaataaa taagttttat agttaatata aggaaacagg
     7141 aggttttatg aaaaattata cgttaatatc gccatgtttt tttggaatgg aaaaaatgct
     7201 ggctagagag attactaatt taggatatga aataataaaa acagaagatg gaagaataac
     7261 atataaaact gatgagtttg gaatagcaaa gtcaaacatg tggttgagat gtgctgaaag
     7321 agtacatctt aaaattgctg aatttgaggc aaaaagcttt gatgaattat ttgaaaatac
     7381 aaaaagaata aactggtcta gatatatacc ttatggagct caatttccta tatcaaaagc
     7441 ttcctctata aaatcaaaat tatacagtac accagatgta caagccatag taaaaaaggc
     7501 tatagtagaa agcttaaaga aaagttattt ggaggatggt ttgcttaaag aagataagga
     7561 gaaataccct atttttgtat ttatacacaa agataaagta accatttcta tagatacaac
     7621 aggagatgct ttgcataaga gaggatatag agaaaaggca aataaagctc caataagaga
     7681 gactttagct gctgggctta tttatttaac tccctggaaa gcaggtagag ttttagtaga
     7741 ccctatgtgt ggttctggta ctatattgat tgaagctgct atgattggta taaatatggc
     7801 acctggctta aatagagagt ttatatcaga aaagtggaga actcttgata agaaaatatg
     7861 gtgggatgta agaaaagatg cttttaataa gatagataat gaatctaaat ttaagattta
     7921 tggatatgat atagatgaag aatctataga tatagctaga gaaaatgctg aaatagcagg
     7981 tgtagatgaa tatatagagt ttaatgtagg agatgcaact caatttaaaa gtgaagatga
     8041 gtttggattt ataatcacta atcctcctta tggagaaaga ttagaagata aagacagtgt
     8101 taaacagttg tataaggagc ttggttatgc atttagaaaa ttaaaaaatt ggtcgtatta
     8161 tttgataact tcttatgaag actttgaata tgagtttggg caaaaagcag ataaaaaaag
     8221 aaaattgtat aatggtatgt taaaaactaa ctttttccaa tatccgggtc ctaagcctcc
     8281 tagaaataat aaataagttt tacatgattt atttgtaaaa tataaaattt tattaaaata
     8341 agtaaaacat tatgatgcct tgaaacgtct aattattgtg aggagatttt tgtgaacaaa
     8401 tttaaagatt tataggatta ggaggagtta aatgaaagtg ttaaagctta ttactgtagt
     8461 agccctgatg atgttatttg ttactggatg taatataaaa actgaatctc cagaacaatt
     8521 agcgaagata cctgactatg atgatacaaa aaaagtattg tatgatggta ttgaccagtt
     8581 actaaaacca gattcatcta tgatattgcc ttctaataca aaagaagtag gaaaaataaa
     8641 taaggtagat ttaaacagcg atggaattga tgaattagta gtatttgaac aaaaagaaga
     8701 tttaagtaat aatgtgagtc aggtagggtt tgtaaccttg agttacaatg gagaaaaata
     8761 cgtacttgga gaccattttt tagaaaatgg cgaatcaata gaatatgcta atttttatga
     8821 tttggactca gatggatata aggaagttat cttattggtt aaatccaaag acaaaacgaa
     8881 tatgcatata tataaagtag tagataatga aataacaaaa atatatgact tggatgcatc
     8941 atggcttcaa aataaagaag attttaatga tatgaaagta aaaattggat atatagatgg
     9001 agatgacaag ttagacatat tgatgcttca tttgaataat aagactaatg aaatgtttgc
     9061 aagtgttgca aattttgatg ggaaaatgaa aatcaaagat tctgttaagt ttgaaaatgt
     9121 aaaaaactta agtgaattat atataacatt aggtaatgtt gcttcatctg ttggaaattc
     9181 ttctgcagct ataaaaggta ttgtacttga tattcctata ttgaaagata ataactatat
     9241 tacgcagata ctttatatga aagatgggaa aattaacaaa gcttttagtg attatgataa
     9301 aactattact aaatc
//
LOCUS       DAGSHT010000133         9257 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGSHT010000133 DAGSHT010000000
VERSION     DAGSHT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.137"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(108..2882)
                     /locus_tag="MJB72_002676"
     CDS             complement(108..2882)
                     /locus_tag="MJB72_002676"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBY8263760.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(3054..4310)
                     /locus_tag="MJB72_002677"
     CDS             complement(3054..4310)
                     /locus_tag="MJB72_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBY8263761.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(4382..5884)
                     /locus_tag="MJB72_002678"
     CDS             complement(4382..5884)
                     /locus_tag="MJB72_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY8263762.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(5975..7165)
                     /locus_tag="MJB72_002679"
     CDS             complement(5975..7165)
                     /locus_tag="MJB72_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8263763.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(7404..8492)
                     /locus_tag="MJB72_002680"
     CDS             complement(7404..8492)
                     /locus_tag="MJB72_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263764.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            complement(8777..>9257)
                     /locus_tag="MJB72_002681"
     CDS             complement(8777..>9257)
                     /locus_tag="MJB72_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBY8263765.1"
                     /translation="SKKINFVVITAFNYFEYAQKALRLNVKDILLKPLDMKEFTKSVE
                     KIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSNISRIFKSNLNTTFIS
                     YLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIEKGMTPKVYKLNTKN"
BASE COUNT         2964 a         1710 c         1082 g         3501 t
ORIGIN      
        1 tgtttagatt aataaaaaat taaatatgaa aatggtaata taaaacaaca ttaaacaaac
       61 aaaaaccgca aagttacaat atatagtact ttgcggtttc ataggaatta atttttacta
      121 ttaaagttca tagttctact atttttcatt gttctaagaa ttagctttac aaattcattt
      181 gacaatagga tagtggatgc aaatagtatt atctttatcc acatatcaaa gtccaaagaa
      241 acagaattaa agaaactctg gaacacttgt gtaaatatta tttgaattat ccctgtaact
      301 actattattt gcaatgccag tttattttta aagaaatttg gtattgtact atttaaacca
      361 aactctctac aatttaatgc attaaaaagg gcactgaatg caaataaaga aaatataaca
      421 gtaccttgtt cttctgatgt agctcccaat atattaaaag ctgattgagt aaatacaatt
      481 gtaattataa gaatagcatt tataataata tttacaaaca tagacctagc tattattcca
      541 gaatgtctat ttattggttt tcttttcaat acataatctc taacaggttc taatcctaat
      601 gctagggctg gaggaccatc cattattata tttacccata ataattgtat tgtagtaaat
      661 ggcatatcct taccagtaag ttgagatatt atagcaatta taaatgctac tatatttaca
      721 gtaagctgaa actgtataaa tctttgaaag ttctcatata tacctcttcc ccattttatt
      781 ccctctacta tagtactaaa gctatcatca gtcaatatta tatctgctgc atttttagat
      841 acttctgttc ctgaaatacc cattgcaatt ccaacatctg cttgactaag tgctggggca
      901 tcatttatac catcacctgt tacagctaca acttcgccac ttttttgaag cgctgaaact
      961 attctcattt ttgtatctgg tttacttctt gcaacaatag atattccttt gatttcttct
     1021 ctcaattctt tatctgtcaa tacatctata tatgttgctt caactgcctt tttcccatca
     1081 tttaataatc ctaattcatt tccaatagct gttgctgtat ttatattatc tcctgtaagc
     1141 atttttacat ctacgcctgc attaaatgct ttatcaatgg aatctttgac cccttctctc
     1201 aatgggtcca ctattcctac aaatccacta aaaactaaat tatcttcctt catataactt
     1261 cttgaatttc ctattaaatt caattcacct tctgaagtca ttgcaacttc tgttttagaa
     1321 ttactcattt ttttataagc aaatcctaat gttctcatag atttaatttg taacttctta
     1381 atctcatcta aaatactttt ttctactttt ggagttattg gaacaatatt ttttccttgt
     1441 tgtacataag aacatttttt taataatact tctggagcac cttttgaaag taatatatct
     1501 gaatcctctt gacgaattaa tgttgacatc ttcttttctt ctgaactaaa tggtatttgt
     1561 gatatcaaat acgtttgttt tctcatttca ttatagttct tatcattatg atataaaagt
     1621 aatgcacact ctgttgcgct tcctatatat ttaaatgaat tatcttcttt ttctatatct
     1681 gcagttgaat ttactataca gttttgttca aaatagctat tactttgata ctctcctcca
     1741 cttatatatt taccatcaac atatgctacc tctaccatca ttttgttttg tgttaaagtc
     1801 cctgttttgt cggaacatat tacagaaaca gaacctatag tttcacaggc ttctttcttt
     1861 gttactaatg catttatctt tgccatcttt tgcattgtta tagctaaggt aatatttatc
     1921 atagttggca aaccttctgg aacagttgca actattaatg caatacaaac catatatgca
     1981 tttttagcag gttctaatga ttgtaagaaa gataatacac tgcttgtatc taaaattaac
     2041 ataccttttc tctgcatctg tataatcata aacatacata ataaagtagc tattacgcct
     2101 gatattcctg ctattttagc acccaacttc cctaatttaa tttgaagtgg agtttgctgg
     2161 tcttcatttg ataaattctg tgctatctta cccatctctg tcttatcacc agtatgagtg
     2221 acaaccatta tgcccctacc atatgctaca agagtccctc caaataccat attgacttgc
     2281 tttgcaggta ttgcatcctg ttcgattatt tctgttttag aataaactac ttccatatct
     2341 aatacagcat cagcattctt agctacatca tcagattctc ctgttagcat atcttctcta
     2401 agtttcaaat ttatagactg tataagtctt ccatctgctg gtatcatatc cccagtttct
     2461 atatatacaa tatctcctgg tactaaatca ttttttgata ttttaattat cttcccattt
     2521 ctaagcacct ttacttctat attttctgtt aattttgaca gggcatgagc tgctttttta
     2581 gattttcctt cagttattat gcctatagaa attccaataa aaatagcccc taaaatacct
     2641 aatgcgtcat gtaattctcc cacaaaagaa cttattactg ctgctcctat gagaattaat
     2701 atcataggtt ctgtcaaact ttctcccaac tcgtcccaaa atgttctacc ttcttttata
     2761 gtaaattcat ttaatccata tctaagcttc ctttcttcta cctcattatc atcaagtcca
     2821 atttctggat ttgtttttaa gtattttaat acttctttgg taggtttatt ataatacctc
     2881 ataactttat cccccttgtt aattatctat aaggaattat atgcttatga tagcttgtat
     2941 attacttttc aaaaaaaata aggtgttgat taatcaacac cttatagtgg ttatatatta
     3001 ttaaatttta tgttacaggc tttataaatt gtgagctata caaatcaaaa tatttaatct
     3061 tcaaaatacc cttctggata taatggaaac ttagatataa gttcttgtgc ctctgcttta
     3121 cattcatcta atacttcttt attatcaata ttttctgcta ccttattcat tatccctgct
     3181 atctgtataa cttctttttc tttcagacct ctttgagata ttgatgtaag acctatacga
     3241 acaccactag ttacaaatgg tgattgtggg tcgtcaggta taacattttt attaactgtt
     3301 attcctacat attcaagagc tttttcaaat tgttttcctg taagattctt acttcttaag
     3361 tcaaccatta ctatatgatt atcagtacct ccacttacaa tactaaaacc atgtgaaatc
     3421 agttcagaag ctagagtctg agcatttact acaacttgct ccattatctt tttaaattta
     3481 tctgtactta aatataatag tgaaaaggct tttgctgcaa ctgtattaag atgtatagac
     3541 cctaatgttc ctggaaaaac acctttatcg agtttctttg catgttctgc tttacaaagt
     3601 acaataccac ttcttggtcc acagaaagtc tttgtcgtag atgaagaaac aaaatctgca
     3661 taaggtacag gactaggtat tactttcgct gcgactaatc ctgcaacatg tgccatatct
     3721 accataaagt aagctcctac ttcttttgca acctttgata ttctttcata atctattaat
     3781 cttggatatg aacttgcccc tgcaattata agttttggtc tacattcttt tgcctttgct
     3841 tcaagagcat cataatctat acgtccagta tttgggtcta ccccataaaa ttcatattta
     3901 taaatgtcat gtaaaaaatt aactgcgcta ccatgtgtca agtgtccacc ttggtcaaga
     3961 cgcatactta aaacagtgtc atttggtttt aatattgatg aatatacaca atagttagct
     4021 gtagaacctg aatacggttg tacgtttaca tgttctgctc caaatacttc ttttgcacgt
     4081 tttattgcta gggtttcaag tttatctgct tcttcagaac ctgcttgaaa acgagcccct
     4141 ggatagcctt cttctgtttt atttgtaaat acacatccac ttaattcaag tacttctgtt
     4201 ggtgctgtac tttcagaagc tatcatctca atattgtgtt cttgtctaac caactcatca
     4261 gctacaatct tgtataactc tgggtctgaa atttttgttg tttttaacat aataatcctc
     4321 ctcttaatta aagaatatat tttattagtt taatcttcat tgataaattt attataatgg
     4381 tctataaaac gtaagatatt cctccaccag gtcctaatgg tatgccaata cccatgaata
     4441 gtaacaatat tattatttct attaatgtaa ataccactga gtatggaatc atattcgata
     4501 tgacagtacc tatacctata tctttatcat atcttctggc aaaggccaat ataactggaa
     4561 aatacggaaa aagtggactt attggatttg ttgaagcatc tccaatacga taagctattt
     4621 gagttaatgc tggattaaag tctagtagca taaacatggg aacaaatata ggtgctaaaa
     4681 tcgcccattt tgcagaagca cttcctacaa ataaattgat aaatccagat agcaatataa
     4741 atcctattat caatccatag ctaggtattc ccgactctgc tagtaatttt gctcctgtta
     4801 ttgataaaat tgttcctaag ttactaagat taaataaatt aataaactgg gatgctataa
     4861 atgctaggac tatatatcca cccatatcac tcatactact acccatcata gatactaaat
     4921 ctttgtcact ttttatcttc ttacttactt ttccatacac taatcctgga gttaaaaata
     4981 taatagtgat tataggaact atacctttca ttaaaggtgc attactagat agtatagaac
     5041 cagtctctgg atctttcata aatgcattgt cacctataca taaggcaact atcactacta
     5101 ctacaactaa caatgataat aaagcgtatt ttataccctt attttcaagt tcactcaatt
     5161 cctgtatatc cttatcattt gttgaatcat tatattttgt aaaacgagga gctataaatt
     5221 tttcagttac aaatgctcct gcaaaagtta aaactatcgc tgatataaaa gcaaaataaa
     5281 gattcattgc tggattgcct tggtaagatg ggtccatcat ttgagctgct ggtattgtaa
     5341 atccagctaa caatacatct gtagtactaa gcataacatt tgctgcaaat ccccctgcaa
     5401 cacctgcaaa agctgcaaac atacctacaa gaggatgtct tccaattcct aaaaacacaa
     5461 gtgctgcaag tggtggtaat attataaatc ctacatctgc tgctgtatgt gataacatac
     5521 ctgcaaatac gattgctaca gttacaagtt tttgtggaac tttacttata ccctttttca
     5581 ttaacacttc cataaatcca gatttctcag caacacctgc tccaagcatt gtaaccagta
     5641 ctaatcctaa aggagcaaat gactggaagt ttgtaactat gctacctaaa tatgattgaa
     5701 tctgctcttt ctctagtaga tttactacct ttacaacttc ttttgtagaa ggatgtattt
     5761 cttctactcc taaattagat ataactgctg acaatattac aacaattata catagtaata
     5821 aaaatatggt tactgggtct ggaagtttat ttcctgctct ctctacaaca tttagtgccc
     5881 tcatcaataa tccactatct ttgttttttt cctgtgaatt aattttcctc aaactaatac
     5941 cccctcataa actttatctc cagaatgctt tatcttaaat tttatttttt aatttatcca
     6001 tataatttat aacagactga catagtacat ttgtacctac caatatagaa tttttatcag
     6061 ctttaaattt aggattatgc cactgatata tacaatcttg ttctttgttg cctaccccta
     6121 accagtaaaa aaatcctggg gctttttcca tgaatataga aaaatcttct cctcctgtag
     6181 aaggtattgg atcaactata gcatcttctg aatacaaatc acacacagac ttacaagcta
     6241 ttgcgtacat ctctttacta tttataacag ctggtaaatc aaataaataa tctagctttg
     6301 cagttgcttg atatccacga gctatctcac aaactatatt ttctaaattt tctgatattt
     6361 tctttcgtac ttcattacta aagcttctac atgtgcctgt catatctacc ttttctgaaa
     6421 ttacattatt tgcagtccct ccatttatag tacctattga tataacagct gattctaatg
     6481 gacttatgtt tctgcttact atggtttgaa tacccattat aattgcactt gatgctacta
     6541 tagggtctat tgttctgttt ggtatacctc cgtgacctcc aaatccattt acttctatct
     6601 taatagtgtc tactgctgcc attaacccac ctaacttaac acctattttt ccacaaggta
     6661 tatctggatg attatgaagt ccaaatatag catcaacttt tggattttcc attactcctc
     6721 gttccactag catcttcgca cctttattga cttcttcggc tggttgaaat ataaacttta
     6781 catttccatg aagactatct ctaatatgag acaatacata tgcacatcct acaaggcaag
     6841 acgtgtgtat atcatgccca catgcatgca ttataccatc atatctggat ttatacttaa
     6901 tatcaacctc ttcatatatt ggcaatgcat caatatctcc tcttagtgct actgtgggac
     6961 catcatactt cccttttaat aatccaacaa ctcctgtttc cattcctaaa tctaatattt
     7021 ctatgtctaa acttactaat aaatctttta tagcttttgt agtcctaaat tctttaaaac
     7081 ttaattcggg gtgcatatga atatctgtaa aaaaatctaa aatatcttct ttatactttt
     7141 ctgagattaa aataagttca tgcatactaa cacctctcca aaattttatt tttttatttt
     7201 ttataattta actacaaatt atataagtta ataatcttta taaagatatt ttttataaat
     7261 ttaattttag tttatattta cattttacta tattatttta attaaatata ttatcatgtt
     7321 ttgcaattta tttaactatt ttgacttttt aaattcggat tatataaaaa atttctttaa
     7381 ataaaaacta ttaataagtg gctctattcc tgactttgtg ttgatttttt tctacatgta
     7441 attgctttat acaccataca gactaaaatt aaaagcccca tataacctgt atatggatat
     7501 aaaatgttta ctaatttacc aaacggtaaa aaacctccta tacaagccaa aatagctatt
     7561 actgttgtta ccaatttaaa tttaggatgt tcatcttcaa caattctatt ggttacagac
     7621 cataaaagtg gaacagctgt tgtatagata cctaatatta atgcaactga aaaacataca
     7681 cctacaatcg gagatatatt atctgctaaa taaagtgatg gtatttcttt catatacaaa
     7741 tttcctatat ctgaaatcat agccaaatac ataactatac cagcagtcat aaacactatt
     7801 ccacctaata aacctcccca aaaagcatcc tttttacttt caatagaagc tcccattcct
     7861 gttaaaaaaa caatcattac aataatattt aatgtattat atattacacc agaaacgatt
     7921 ggactttctg atgcttttgt aacttcaata tttttcatag cttctccaac catagataaa
     7981 ccgtcaaaat gcttaaacaa gcttattaat ccaacagcaa ttgtaaatac tgctatcact
     8041 ggtccaatac gtccaagtat gcttactaat ttttctaaac ccatagacac tgtaattaaa
     8101 gtagcaacag ccatcaatac tcttcctaag taaggattta taccatagta ttcagttaaa
     8161 gttgagcctg cacctgaaat cattactata agaatcccaa ataaaaatat tggactaaac
     8221 cattcgtaaa attttcctaa aatcttccca caataaaaag tgtaaattct tgttggttct
     8281 ttaagtttta attctctacc tctagttata acttcagcac caagaaatga aaacaatacc
     8341 attgatatta atgctccaat taaacccata tacccttgtg aggcaaaaaa ttgtagaatt
     8401 tcttgaccag tagcaaaccc agaaccaatt gctgttgcta gataagcgcc tgcaatacag
     8461 ataatatttt tcatattcac tttgtctgac atattaaatt acctccatta taaagttacc
     8521 ttcctttaag aaatataaac tctcagtgaa ttaaatgaaa attctgtatt tttaaaacag
     8581 tatttaattc acttttttta ttttgtatat ctttttcttt ttatttatat atattttttt
     8641 atataaatag gtacagcttt tataaggttt aatattatta aatatgttta aagcgcttta
     8701 atttttatca taattaaagt atacttattc agacacactc aaacaattat ctatattgac
     8761 tttttattta tcaatttcaa tttttagtgt tcagtttata aacttttgga gtcattcctt
     8821 tttctatctt aaagttttta taaaagttat ttaggctatt atatccaact atttctgcaa
     8881 tctcatttat actttttgta gtattttcaa gtaactcaat agatttttct attcttatat
     8941 gatttagata tgatataaaa gttgtattta aattactttt aaaaatccta gatatattag
     9001 atggattggt taaaaataat ctagcacaat catttaactt taagtcattg ctatagttta
     9061 gatttatata ttccagtatc tcatttaata aatcattatt ggtatattga tatccaataa
     9121 ttttttctac agacttagta aattctttca tatccaatgg tttaaggagt atatctttta
     9181 catttaatct caaagctttt tgagcatatt caaaatagtt aaatgcagtt attactacaa
     9241 agtttatttt tttagat
//
LOCUS       DAGSHT010000134         9193 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGSHT010000134 DAGSHT010000000
VERSION     DAGSHT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.138"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1040
                     /locus_tag="MJB72_002682"
     CDS             <1..1040
                     /locus_tag="MJB72_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBY8263766.1"
                     /translation="GCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIFVEFPIIFIGY
                     SVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHTIAFDNKMITM
                     TKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIRVGSVDDTRIK
                     DEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAYPSLIKQYNKL
                     PLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVYGIWNDEHQSF
                     EKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRRLIRIYDYMKY
                     SNMQKSLGYSE"
     gene            1303..1809
                     /locus_tag="MJB72_002683"
     CDS             1303..1809
                     /locus_tag="MJB72_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBY8263767.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            2049..3179
                     /locus_tag="MJB72_002684"
     CDS             2049..3179
                     /locus_tag="MJB72_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY8263768.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(3540..5228)
                     /gene="ilvB"
                     /locus_tag="MJB72_002685"
     CDS             complement(3540..5228)
                     /gene="ilvB"
                     /locus_tag="MJB72_002685"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBY8263769.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSIEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(5281..6276)
                     /gene="ilvC"
                     /locus_tag="MJB72_002686"
     CDS             complement(5281..6276)
                     /gene="ilvC"
                     /locus_tag="MJB72_002686"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBY8263770.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDHT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            complement(6580..6819)
                     /locus_tag="MJB72_002687"
     CDS             complement(6580..6819)
                     /locus_tag="MJB72_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY8263771.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     misc_binding    complement(6932..7189)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7350..8111)
                     /locus_tag="MJB72_002688"
     CDS             complement(7350..8111)
                     /locus_tag="MJB72_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBY8263772.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     gene            complement(8116..8937)
                     /locus_tag="MJB72_002689"
     CDS             complement(8116..8937)
                     /locus_tag="MJB72_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBY8263773.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            complement(8979..>9193)
                     /locus_tag="MJB72_002690"
     CDS             complement(8979..>9193)
                     /locus_tag="MJB72_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY8263774.1"
                     /translation="QIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKNGNLVISF
                     DEYEVAPGYMGAVEFVIPNKVIKRLR"
BASE COUNT         3206 a         1525 c         1030 g         3432 t
ORIGIN      
        1 atggatgtgt ttcttctcca aatagtattg taataaatga agctgattat atagactttg
       61 acagcaaaag tgcctacttg gcagctaagc taatgactat atttgttgag tttcctatca
      121 tatttattgg ttactctgtt acagatgtaa atataaaaaa aatacttaat gctatagtta
      181 attgtctatc taatgaaaat gctaaaaaac tcgaggaacg tttcatattc attgagtatg
      241 aaaaaaactt taatgatata gaaatatcaa gtcatacaat tgcttttgat aataaaatga
      301 ttactatgac taaaattaaa ctggaggatt ttaatttact ctatgaggca ttatctgaaa
      361 aaaaatcaaa attaccagtt agaatattaa gaatgttcaa acaagaattt tacgatttca
      421 caattactaa caaacctact gctaaaattc gggttggtag tgttgatgat acaagaataa
      481 aagatgaaga tttagtttta gcagttggca aggcaagtga ttttggtctt aaaggattaa
      541 aaggattaag ttttgatgaa tggtatcgtg atatagtcat gaatgactta gaattttcat
      601 cagatgaatt acttgaatat gcttatccat ctttaattaa gcaatataat aaattaccat
      661 taaataaaca tctttttaat agcacacttg aatttccaga ttataggaca attgcactag
      721 aaagtgactt tgaaaatatt attagcaata gtattaaaaa aaatagaaat aatacttaca
      781 taaaaaatag aagtgtttat ggtatttgga atgatgaaca tcaatcattt gaaaaagcta
      841 caagacttat tgctttttta gaagaaaaag aaattgacac tgaacagtta gaaaatctac
      901 ttaagaaaat atttgaggaa aatcctaata ttttagagtc tgcaaaaaca agcgaaaaaa
      961 caaatttaag aaggcttatt agaatatatg actacatgaa atactctaat atgcaaaaga
     1021 gtcttggtta tagtgagtaa aactctaccc accgctccaa aactccttta atcgctttaa
     1081 gcacaaatac aaatttagtg ctaactatac taacacacat ttgtaagtgc ttacatatat
     1141 tatatgtaat tatattataa tcatatacta taatccatat tttgtctaca gctaaaatta
     1201 tccaatcttt tttggataaa aaatgaatat ttagagcagt tcacacctgc tctttatata
     1261 taacaaaaat caaaaataca ttctttttat agggggattt caatgaacaa actagacgca
     1321 cttttagact tagcaaataa tgaagagata gaaatttact acactgacaa aatagcagat
     1381 gacataaaag gattgtatat aaacagacaa ggactaaaga ttatatcatt acttaattca
     1441 ttaaaacaaa acaatgctaa actaatagaa atcttagcag aagaattagg acatcatttt
     1501 accagtgttg ggaactatgt atcttcaaaa aacagttaca aaaataaaat cttgatagac
     1561 aaaactgaaa acaaagcatt aaaatgggca tgtgaatttc ttataacaga agaagaaata
     1621 atacatgtta ttaattcaca cgctacaagt gtatacgaaa tagctgaaga attacaagtt
     1681 agcatcaact tcttactaaa aagattagaa tttctatcaa aaaagaaaag catgttggac
     1741 ttaggaaata atagattttt agtattaact aatttgccaa atttctacat atatgaggat
     1801 attttttaaa ctcatttatt ctacttttat agatttttta cttaataaat atatatttca
     1861 atattattat aataaactac acataaaagc taaaaaattg taagaatatt aagaaaataa
     1921 ttaagtaaaa accagataaa caaaattaag ataatattgt acataacaaa agtatataaa
     1981 gagcagttaa tctgctcttt tatataaaca ccaaacaaac atacattcta aaagggaggg
     2041 atactattat gaaaggtgga gtaagaaaaa gaagtaacaa atggtattac tactttgacc
     2101 taggcatagt agaaggaaaa agaaaaaaag tagaaagagt tggaggcaat actaaaaaag
     2161 aagccgaaaa agccttaaga gaagcactaa atgaatatga aaactctggc atagtatttg
     2221 aagaaagcaa catcagttta tcagactact tagatttttg gtacaaagaa tatgtcttac
     2281 ttaactgtaa atacaacact caagaaagct accgaataaa catagaaaaa catataaagc
     2341 caaaactagg agcttacaaa gtaaaagctt taactcctgc aatactacaa aacttcataa
     2401 ataaaaagta caaagaggat tactctcaaa atacattaca agtattaaaa gccatattac
     2461 ataggtcatt aaaatcagca gtccatcctt acaaacacat acgagaaaac cctatgcaat
     2521 atgtaagcat accaaaaact aaatctaaaa cagaaactaa taaagttaaa actattacat
     2581 tagaagaatt taatcaaata ctaaatatat ttcctcaaga ttcatttcaa cgtatagttt
     2641 tactaattgg atttcatact ggtatgcgaa gaggtgaaat tattgcacta aaatgggata
     2701 atatagacct taataataaa actatcacag taagacatac tctgattaaa aagccaaatg
     2761 gaatgtttga attagggcaa ccaaaaacag aaagctcttg cagaactata tttacaggtg
     2821 acactttaat aaaggcatta aaagaacata aattatatca aaagaaaatg aaattaaaat
     2881 atggagaatt ttactttgat agtgactggg tatgtaccaa agaaaatggt caacaagtga
     2941 atactcacac tttagacact atagtaagac aaattcgagt agctttaaac aatgacttcc
     3001 attttcattc tttaagacat gcacatgcta ccctattatt agaaaatggt gctaacatta
     3061 aagacataca aaaccgtttg ggtcatagcc aactatcaac tacaatggat acctattcac
     3121 atgtaactga taaaatgaaa aatgaaactg tagatatatt tgaaaaaatt acaaattaga
     3181 gtttgccacc caaaaatata atacggtggc aaatgggtgg caaaatctaa tttatctatt
     3241 ttaaaagcta aaattatcaa atttatataa tcatctatat acttgtaatt tcaaggcttt
     3301 agagtatata acaaccataa ctaatataag gtattaataa taaatctaac aaataaaact
     3361 taatatttac ttaagttgta atactattcc aaattgtata attaaaattt aataagttct
     3421 taattatata gtcattgatt tatttgaaaa aataaaaaat gccaatctat ctctaaacaa
     3481 caaaaactga ataccaataa aaatttaata aaaaatttta tcaatattca gtataagttt
     3541 tagttacata tcaaattttc caatgcatca ttaggtgcta caataggata aacatcataa
     3601 tccttatcta tatcacattg caaaaataca ggtttattaa aatctatcat atctagcgcc
     3661 tttcctaatt cttctatact ggatacctta tatccatcaa tattatatgc atttacgagt
     3721 ttaacataat ccacattttc attaatgtca gtttgagagt atctttgatt ggaaaataac
     3781 ttttgccatt gccttaccat tcctaatgtt ctattattga gtagtaaaat aagcataggt
     3841 acattataat ttgcaactgt agctagttca ttacagttca ttctaaaact tccatcacca
     3901 gtgactaaaa ccacattttt atctacatta ccaactttag ttccaatagc tgcacctaaa
     3961 ccaaatccca tagttcctaa ccctgctgat gttataaaac ttttattacc tttaaaattc
     4021 cagtacttag cagcccacat ttgatgttga ccgacatcag ttactaccac agtgggtttc
     4081 tttaatgttt catatttttc atttattttt ttaagtatat tttgtggatg aaattcatat
     4141 gtttgtacac cctcactctt tctaaatctc tttatctctt ctttccaatt gctattattc
     4201 ttactttcaa ccctttcaat taataaacta aggactagct ttacatctcc aaccaaagat
     4261 acattagatt ctatattttt acttatttca gatgggtcta tgtctatgtg tattattttt
     4321 gcatttttag caaattcaga acttttactt atcactctat cactaaatct cgcacctatc
     4381 gctattacta aatctgaatt agaaagtgct aaattacttt ctctacttcc atgcattcca
     4441 accattccaa gagatagttc attctttcta tctatatttc caagccccat aagtgtattc
     4501 agtacgggtg tatctatctt agttgcaaat ttttcaagaa tttcctcact gtctgaagat
     4561 ttaacacctc cacctgcata tattacaggc tttttagatt cttttatgat atctattgct
     4621 tcatttaaaa gcttaatatt agtttcatca tctagatcaa aatcactctt ataatccata
     4681 taatcatcta tttgacataa atcatagtcc tcaccactaa aatccatttc tgctaaaaat
     4741 aaatcttttg gaacatctac aagtacaggt ccttttctac ctgaatttgc aactctaaaa
     4801 gcttctttta ttgtaggaac taattcttta gtgtttctaa ctaaataatt atgttttgtc
     4861 atagataatg ttgctccagt tatgtcaatt tcttgaaaag aatcttttcc taataaacta
     4921 gttggcacct gccctgaaat tacaaccata ggtgaagaat ccataaaagc tgttgctatt
     4981 ccagttatgg catttgttgc tcctggacca gatgttgtaa aacaaactcc tactgtatta
     5041 gtacttcttg cgtacccatc tgcagcatga actaaacctt gttcatggga tgtccttata
     5101 tgtttaaaat catctgaata atcgtataga gcatcataaa gaggtatcac ggctccacca
     5161 ggatatccaa atattgtgtc aattccctct ttctttaaac attctaaaat tacctttgct
     5221 ccattcatac gcactactat acaccactct ttccctcaaa ctaatccatt taatatatat
     5281 ttattttaag aatgacatca tacttcttag atttctacca acttcagcaa tttctgtatt
     5341 atattcttct tctctagttt ttaggaagtt ttcacgtcct tcttcatttt ctttaatcca
     5401 cgcttttgca aattttccat tttgtatatc ctctaaaaca ttttgcatac cttgttttgc
     5461 agcatcagtt attactcttt taccagatac ataatctcca tattctgctg tgtcacttat
     5521 actatatctc attctttcaa atccaccttc atatataagg tcgactatta gtttcatttc
     5581 atgtaaacat tcaaaatatg ctacttcttt ttgataacct gcatctacaa gcgttttata
     5641 tcccaatttt ataagttcac ttattccacc acaaagtact gattgttctc caaataaatc
     5701 tgtttctgtt tcttctttaa aagtagtttc taaaacacct gctcttgttg ctcctatccc
     5761 ttttgcatat gctaaaactg tctctgtagc ttttttagtg tggtcttgat atactgcaaa
     5821 tagtgcagga actccacttc cctttgtaaa tacatttcta actaagtgac caggtccttt
     5881 aggagctacc attacaacat caacaaattc tggtggttgt acttgattat aatgtatatt
     5941 gaatccatga gcaaaagcta atgtctgacc ttcttttaag ttatccttta cactctcttc
     6001 ataaactttc ttttgttttt catcaggcat taacatcatt gtcaaatcac tttcttttgt
     6061 agcttctgct acactcttaa cttcaaatcc atcttctttt gctttttgtg ctgatttact
     6121 tccgtcataa agacctatca ttacatgtac accgttatct ctaagatttt gtgcatgtgc
     6181 atgtccttga cttccataac ctaaaactgc aaccttttta ttttttaaaa cctctaaatc
     6241 cacatctttt tcataataca ttcttgccat tttcatttcc tcccaatact ttttcttatt
     6301 tttatgatat ttatttttat tagaaattca tactcattat cattgtttta atattattca
     6361 acagatttaa attctaaatt tatataatct tacaattata attttagaat tttcagtttt
     6421 tattagctac aacaaactaa taaatttatt aactgtgata aactaataaa ttcaatgaca
     6481 cttatatttt aatctaagat ttttcttatt taatatttat ctttagtttt ttattttaaa
     6541 ataagcatat ttatctttag ttttataaaa tatatttaat taatgtctca ttctttcaac
     6601 agttatctct cttatatcat gtagtttttt catatggttt aagacagaat ctaaagatgt
     6661 cttttcttca tttactatta attttatacc tgaattattc atattatcta cagtcatact
     6721 tattgattgt atatcaacct ctcttcttct tagagtcata gccactctta aaaaagaatc
     6781 tattccttgg tttaatttag catatacagt attttccata aaaatccctc cttattttta
     6841 ttattttttt attaatttta tatggattaa atttatttat atcgcttaaa ataaaaaaac
     6901 tttcatcttt ataagcaata taaatattac ttataaggac gaaagttata ttccgcggta
     6961 ccaccttatt tgtagatata aaaaatacat ctacccctct tttttcaggt cgggtaatta
     7021 attaccttaa ccttagtcat gtaacgttga catacgtttg tacttactaa aaatattcag
     7081 tacaaaagct ccagagtgat cattatacta ctgttaacac caattctcag caacattggt
     7141 tctctgtgga taacactatt agtattgttg tctctgtcat tgcttttaat tttttaattt
     7201 tatattttaa ttctgttttg ttgttaatat agattatatt ccctatcatt tacaatgtca
     7261 atgacttttt ttaaaatttt cagattattt atttttttca taataatact tcttttatac
     7321 tttacgccta tattttggaa taatcgtttt cagattatta ttttatatcc atttattttt
     7381 agccaatttt ctttatcttt ataatcgggc ataatggatt ttactaaatc atagaatttt
     7441 ttagagtgat tcatatgtac taaatggcta aactcatgta ttattacata atcaattaca
     7501 tctaatcttg ccatagatat ttttgagttt atataaatat ttttttgagc tgtacaactt
     7561 ccccatctct tattttgttc ttttacctta acactcgctg gaactaattg cttcattatt
     7621 tcacaattta atttaagaaa atctattctc tctaatacta ttctttcact ttccatctta
     7681 taccaagatt ttaaagattt tttaataaaa tctgtacttg tatcatttgt ctgaacaact
     7741 atttgatatt cgttaatagt tattctacag ttactttttt tgacagaatc tctcaacagt
     7801 tcttttataa ctagatggta ctcttcacct aaataaagaa atttttcgcc agttataaac
     7861 attctttgtt tatatacttc ctctcttcct ttatattttt ctgacttttt aagtatccag
     7921 tctccttttt caataattat atcttttatt atttttttgg atattttagg aggactcatt
     7981 acagttattt tgccaaatgg atctattttt atagaaatat tttttctatt tttatatact
     8041 aaaactgctt ttatttcaat ccctctaaaa ttaaccttta aagtatcaat atctttactc
     8101 cctgtgttca ttagtttatt tttccttatt agctctacaa tttataagtt cagcagctat
     8161 acatacagct atttcagctg gtgtttgagc attcaatttt atgcctattg gtgcatgtac
     8221 ttttgataat tcttccttct taaaaccttt tttcatgagt ttttcataag tcaatccaat
     8281 ttttgttcta cttccaatca tacctatata accagcctct cttttaagag cccattctaa
     8341 agcacttgaa tcatatagat gacctcttgt taaaataatt atataactat tttcgtcagt
     8401 gggaaaatct ggtatgtttt caaaagaatc taaaactact acttctgatt ctggaaacct
     8461 ttcatgatta gcaaattctt ctctatcatc tattactact gttctaaatt ctaaaagaga
     8521 tagtatcaca gcaacatctc ttgaaatatg acctgcacca aatatatatg ctttacttgt
     8581 attttccttt agtctcttat taaatttttc tatattactt tttaatttac tatctataaa
     8641 atctatttgt atttctccag tacctccaca tatcattcct agttttgcca catcagattt
     8701 gtttagagta aatttataca acattgattc tctgtttttt ataacattaa ttgcatgttt
     8761 tatacattct gcttctacac tacctcctcc tactgagcca aatatagtag aatcattttt
     8821 cacaatcatc attgctccag tttcacgtgg tgttgaacct gaatgagacg ttattgtagc
     8881 aaatgcgaaa tcctcattat tttctattaa tcttattgct tgttcatata tattcatata
     8941 attctcctta ccagtctatc taaaaatctt ctatatatct atcttagtct ttttattact
     9001 ttatttggta tcacaaattc tactgctccc atataaccag gagctacttc atattcatca
     9061 aaactaatca caagatttcc atttttatta aaataaaaat cttgatactt atttatagat
     9121 tcaaaatctt tcactggcga gtctttatct tggtctataa aataagattt tgtatcatct
     9181 ttcttaattt gat
//
LOCUS       DAGSHT010000135         9191 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGSHT010000135 DAGSHT010000000
VERSION     DAGSHT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.139"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            393..1598
                     /locus_tag="MJB72_002691"
     CDS             393..1598
                     /locus_tag="MJB72_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY8263775.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="MJB72_002692"
     CDS             complement(1669..1890)
                     /locus_tag="MJB72_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263776.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="MJB72_002693"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="MJB72_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBY8263777.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="MJB72_002694"
     CDS             complement(2643..2993)
                     /locus_tag="MJB72_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBY8263778.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="MJB72_002695"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="MJB72_002695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBY8263779.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="MJB72_002696"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MJB72_002696"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="MJB72_002697"
     tRNA            complement(5068..5159)
                     /locus_tag="MJB72_002697"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="MJB72_002698"
     CDS             complement(5160..5615)
                     /locus_tag="MJB72_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBY8263780.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5936..7207)
                     /gene="serS"
                     /locus_tag="MJB72_002699"
     CDS             complement(5936..7207)
                     /gene="serS"
                     /locus_tag="MJB72_002699"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBY8263781.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7333..7538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7666..8547)
                     /locus_tag="MJB72_002700"
     CDS             complement(7666..8547)
                     /locus_tag="MJB72_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBY8263782.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8559..9068)
                     /locus_tag="MJB72_002701"
     CDS             complement(8559..9068)
                     /locus_tag="MJB72_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBY8263783.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2943 a         1598 c          973 g         3677 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggttc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ttacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtagcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatggtact gtcctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagta
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcctca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataacg tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctactataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 ggtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaataca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 taccaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt ttttaaaatt agttctaaaa taatttttag atattcatac
     5761 aagagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5821 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5881 ctcataatat attctcttca tcaaagcata ctctatatat acaattaata taaatttatt
     5941 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     6001 cctgctgata attttctaat atagcagcca aacatcttcc tattgctaat ccagaaccat
     6061 ttaatgtatg aacatattct gcttttgatt ttttatctct tttaaatctt attcctgctc
     6121 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtaac
     6181 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6241 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6301 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6361 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6421 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6481 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6541 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6601 ttctattggc cataaatgga ggtaagactt ccgtatagcc atgcttagct gtatgagtat
     6661 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatccccta tataaagtaa
     6721 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtaccta
     6781 tatcccaatg cgctttatgc tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6841 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6901 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6961 tcttatctga taactctttc aatcttgcct tttcttctgt tacatctttt ccttctttta
     7021 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     7081 gaagtatttc tcttctttta ttatctaatt ccactacagc atcaaggtca aattctcttt
     7141 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7201 ctaacatatt aattcctcct aataattttg ataatataag ttataattcc aacttaattt
     7261 acaaacatta tacttgtaaa ctcttagttt aaacaaattt taaaaataaa aaaagacctc
     7321 cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt tgccacccta attgactata
     7381 ctaaaatgta taatcctctc agacctataa cgtaggtaaa cgtctagctt taactagaag
     7441 ctctagagta gattcaaaaa ccattgtaat gagttttcac catccactca ttctctgaat
     7501 acaatctgtt ttttactatt ttccttcaca gctttttaac taattgtttt atttgataat
     7561 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7621 tttaattttt tatattaaat tcattttttc tatttaatta aaaccttatc ttttatccat
     7681 tgtatttaga ttacctgtat tattaaataa taatgaagaa ggaacaaatg gaacttttat
     7741 atcattctca tcaaatttat tttttacctt ttccattaca tcaaaatata catcccagta
     7801 atcatcagtt tttacccaag gtctagcaac tatttgcatg gtgttatttc ctatagagtt
     7861 taaacctatt ataggctttg gatcatttaa tattcttttt tcgtttgtaa aaatttcatt
     7921 taatatagac ttaactacat ctatatcact atcatatgaa atattatatg aaaaatctat
     7981 tcttcttttg ttttgatgtg tgtaattaat tatattatta cttgtcagtt gaaaattagg
     8041 tataattata gttttattat ctggcgtctt aagaacagtg ctaaatattt gtatgtcaga
     8101 aactgtacct tctacacctg aaccttgaat ataatcacct atattaaatg gcttaaaaaa
     8161 taaaataatc ataccagagc ctaaatttga taatatttct ttaaaagcta aacctatact
     8221 aaatccagca gctcctaaca cagctacaac tgatgtagtt tgtatcccta taatacctaa
     8281 gcttatcaat gatataatag ataacataga tatatatatt ccatatacaa taaaattatt
     8341 tacaccttta ctcattccat atttgattag aaactttgaa accatccttc ttactatttt
     8401 agatatataa atacctatca caaatacaat tattgcatat ataatatttg gtatattatc
     8461 actaaaatta tcgagcatat tttctaaagt tctcttagtt atattgctat ttgttgaggc
     8521 agtcttagcc aatgataaaa aattcattca actcctcctt atgataatat tccgttgtcc
     8581 tttaactctt ttactatagc ttctatagtt tcttctttta ttgcctcaat tgtatgaata
     8641 tggctacatt ctgtcagaac cgataattgt ttaaattcat ttgtctcaac tttcttcata
     8701 aagttgtcta tatccatttt agaataaata ttaagttctg cagttatctg accatatgtt
     8761 ggatgattta cgatgacatc ttttataata ccacctaaat ccacaattat ctttaattca
     8821 tcataaattt ctttagaatc cttatgattt ttacacttta tttgttttgt aaaatacggt
     8881 tgattatata ttatatatcc ttgaggtgta gctattattt caaatcctct agctcttata
     8941 agggctatat cttgaactat tacttgccta cttacattta atagttcagc tagtgttcca
     9001 ccttttacag gatgcttaga ttcttttaaa atatctatta atttacttct tctttcatta
     9061 gaattcatat tacaccttct ttccaaataa aaactttacc tatactatat aattttatat
     9121 tttatcttat tataaatcaa tattgacttt atattctatt taatcttaac ttttataata
     9181 aataaaaaag a
//
LOCUS       DAGSHT010000136         9127 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGSHT010000136 DAGSHT010000000
VERSION     DAGSHT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.140"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(91..777)
                     /locus_tag="MJB72_002702"
     CDS             complement(91..777)
                     /locus_tag="MJB72_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263784.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(935..1999)
                     /locus_tag="MJB72_002703"
     CDS             complement(935..1999)
                     /locus_tag="MJB72_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBY8263785.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            2261..2932
                     /locus_tag="MJB72_002704"
     CDS             2261..2932
                     /locus_tag="MJB72_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263786.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            2934..3842
                     /locus_tag="MJB72_002705"
     CDS             2934..3842
                     /locus_tag="MJB72_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8263787.1"
                     /translation="MFILAFIICTVVIFIVSTKINQKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            3919..4605
                     /locus_tag="MJB72_002706"
     CDS             3919..4605
                     /locus_tag="MJB72_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263788.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            4602..7157
                     /locus_tag="MJB72_002707"
     CDS             4602..7157
                     /locus_tag="MJB72_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8263789.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(7351..8550)
                     /locus_tag="MJB72_002708"
     CDS             complement(7351..8550)
                     /locus_tag="MJB72_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBY8263790.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            8669..>9127
                     /locus_tag="MJB72_002709"
     CDS             8669..>9127
                     /locus_tag="MJB72_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8263791.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIE"
BASE COUNT         3477 a         1179 c         1282 g         3189 t
ORIGIN      
        1 gttattagat aaattgagat actaaataaa atagctgtaa aaattatacc aacagcaatt
       61 attaaataaa tagaactaaa agaaaatttt ttaaaattca tttctttcac ctaaccacat
      121 atatcccata ccatataccg ttttgatata cttaagattg tctttttcaa ttttgcttct
      181 aatcctactt ataacagatg ttaaagtatg ttcatctaca aagttagcat ctatatccca
      241 gagtttttct aaaagtactt tccttgttaa aatactttta ggattttcag taagcagttt
      301 taaagtacga tattctaatg gtgtaaatat aatttgattt ccatttatat tggcagataa
      361 ctcagaaaag ttaatttcaa gatatccatc cctatagtaa tcttgttttg tttttattgt
      421 aagatgattt aaaaaagctt taattttttg tttaaatata ttgatattaa atggttttgt
      481 tatataatcc aaagctccaa gttcatagcc tttaatcata tcacattcca tatcattagc
      541 agttaaaaaa attattggta tattgtaatt tccctttatt tcattacata attcaaaccc
      601 acttttatct ggtaaattaa tatccaaaat tattaaatca aattcacatt caaaaataga
      661 cttcagtgca gatttagctg taaattttga agtaattata tatccatctt caattaggtt
      721 atatgataga gttttattta atatagaatc atcttctact actaatattt tttccataca
      781 gttactcctt ttagtttaat attttataat gtcaattata gactaaaaac attgctgaat
      841 tgttataaac gagaaaaagt atttaaaaat ttatatattt aataaagaac ctgtcctatg
      901 ataaattcta gtacagattc tttattaaat aaaattattt caaccaagat ggatttccag
      961 gtataaatat tcttttacca aattttgaag tatactcatc taaagctttt tgaataagct
     1021 cattattacc cttaaatgaa gtagtattct ttatagtttc gagattttgg ctaaatgggc
     1081 aagcagatat acacatacca caatctgtac ctaaatatct ccatttaata tagcatgtct
     1141 catgttcaat tacccaatta tattttccat catcaccaat tttaatatca ttagaaagcg
     1201 aatgagaagg gcagttaaaa gagcattttt tacagacctt acaaaaatct tctagtccaa
     1261 aatcaacata ttcatcttct aaaagaggaa tatctgtagt aacaacacct aatctaagtc
     1321 tagagccata atctttattt gtaagtatag catttcttcc tatatctcct aaacccgcat
     1381 cacgagctat aaaaacaggc attactaaat aattagcatc catatgattt ctagcatcat
     1441 atcctaaatt tcttatatag taacttaaaa tcatgccgac gatagatgca tccacatagc
     1501 actttgatgt ttctataact tcacatatca ttggagctct atttatcatt tctttatcca
     1561 tctcacatcc aaatacaatt gcatacttat gagttagatt aatttcttca ccgtaatttt
     1621 cttcatgtct acccctattt gtataaatat gatagtcttt taatttagtt ataccaacta
     1681 cagaagctcc atactgcttt gcaagaccct taattttttt tgtcattgac tttgcatcta
     1741 catctacttt agtatcagaa acttttcctt cgcataaaga tttgatatca gataaaaaat
     1801 cgaatgcact acttgccatt ggagagttta actcattgta agtcattgtt ccttcactac
     1861 ataaatttgg tctatttctt atgctatcat caatttcttt tttgtcagga tttttcttat
     1921 aatagtcatt ataagcagta ctatcttttt tgtaatttgc tcttgcaaac atagtatttc
     1981 tctcatcaac ccttttcaat tctattcccc ctagtaaaaa tatatttaaa aaatataact
     2041 ttattgttgt ataatttaat tatataagaa taaagttata ttttcaattt aagcgtaatt
     2101 aaattgggaa atatataact atatgcttga ttataatgtt tttattttga aaacaaattt
     2161 aaatacaatc tttttagtga aataaataaa attaaaatat ttttagtatg ataatgcaag
     2221 aaaataatta tttatctaaa aaatctaaag gagagtaaaa ttgaacttat taattattga
     2281 agatgatata aacttaaatg aaggtctttt ttatgcattt gagaatgatg gatttaatgt
     2341 atttaaggct tatacaaaac aagaaggatt aaatatattt aatagtaaaa atatagattt
     2401 tataatctta gactgcaatc ttccagatgg agatggattt gatgtatgtc aaataataag
     2461 ggaaaagtca gatataccaa ttataatgct tactgcaaga gactcagaaa ttgatgaagt
     2521 aaaaggatta gaaatagggc ttgatgatta tattacaaaa ccattttctt tatctgtatt
     2581 aaaggcaaga gtcaaagtag caataagaaa aaaatcgaat aataaagtaa tatattccaa
     2641 tggaataaaa ttggaccaaa agttactaaa agtatataaa aataaagaat gtttggaact
     2701 tagctctgta gaatataagc ttctaagtta tctaattgaa aataaaggtc aaatattatt
     2761 aaaggaacaa attttacacc atatttggga cagcgaagaa aattatgttg atgataatat
     2821 tgtatctgta aatataagaa gacttagagt aaaagtagag gatgacccat ctaatccaaa
     2881 atatataaaa acagcttatg gaatgggtta tttatggaat gaggtagaat aatatgttta
     2941 tattagcctt tataatatgt acagttgtta tatttatagt atctactaaa ataaatcaaa
     3001 aaagatatga tgaattaaat ttgatgttaa atcaaatatt ggaaggtaaa gaagttactt
     3061 atccagatac gaaggataca agagcgtcaa aaatttctca ccaagtaaaa aaaattaagg
     3121 atatgataga aatagaggta gaacagtcaa agtcagaaaa agaagctatt aaaggtttaa
     3181 tatcaaatat gtcacatcaa ttaaaaactc cactttcaaa tataaccatt tattgtgaac
     3241 ttctggagaa tataaatatt tccacattac agaagaagga gtttttacaa aagatgaaaa
     3301 atgaaacttt taagattgac tggttgttac aatccttatt taaaatgact aaattagagg
     3361 atggagttat agaatttgaa gtagaagaac tactaattaa agatactctt attcaaagta
     3421 tcagtactat ctttaataaa gcagaagcta aaaatataag gataaatctc gaaccatttt
     3481 cagacataaa gttagttcat aataaaaagt ggaccataga agcaattgta aatgttttag
     3541 aaaatgcaat aaaatactca ccatcagata gcacgattac tatttctgta ataaaaatgg
     3601 aactatatac gaagattact attaaagatg agggaatagg tattgatagt agagaactaa
     3661 atgatatttt taaaagattt tacagaagta aaaatgttga aaaccaaaat ggcacaggga
     3721 ttggtctata tcttacaaga ttaattttag aaaaagaaaa tggaaacatt atagtagaat
     3781 ctaaactagg aagtggatgt tgttttagta tcttcttaca gaattgtaag agtttaaact
     3841 aattttgtag gatgagtgta attatcgtca cttacaatat tttatagaac atatatctgt
     3901 attgaaagga gaaattaaat gagtatatta gtgacgaata atttagtaaa acactatgga
     3961 aaaggtgaga ctaaagtaaa agccttaaat ggagtaagta tagaaattga acgagataca
     4021 tttactgcta tagttggaac ttctggttct ggaaaaagca cattgttaaa tattataggt
     4081 ggattagata atccaacctc aggtgatgta ataattaaag gaaagaacat atctaaaata
     4141 ggaaaaaaag atttgacagt gtttagaaga agaaatatag gatttatatt tcaaaactac
     4201 agtttaatgc ccgtattaaa cacttatgat aatatagctc ttcctgttac attagataaa
     4261 ggaaaccata tagaccatga atatattgaa atgctgatga agacattggg aatttgggat
     4321 aagcgtttaa aatttccaag cgaactatct ggtggtcaac aacagagggt agcaattgct
     4381 agagctttgg caaataaacc tgcgcttata ttagcagatg aaccaacagg aaatctcgat
     4441 agtaaaacta caatggaagt ggtatgttta ttaaaagaaa gtagtgcaaa attccatcaa
     4501 acgatactta tggtaacaca caatgaaaat atagcacaag tatgcgactc aattatccat
     4561 attgaagatg gagttgtggt gaatactggt ggtgaagtac tatgatgtct ctagtaaaat
     4621 tttcaattca atatataaag cattataaaa aacaatctat atctatagtt ttaagtatag
     4681 tactatcagt agcattattg acgggaattg gttctttagt tcatagtgca gacaaaagta
     4741 gaattgaaaa gataagggaa gatagtggag attatcatta tttctataaa gtagataagg
     4801 aacaacttaa taaaattaaa aaaaataaaa aatcaaagga atacactgta gatagacttg
     4861 gcgttacaaa acctaaaaat cagatagatg aaccttttat tatagaattt ttaaatgctg
     4921 attcttcata tttagacatg acaggtagaa aattattaaa aggaactctt cctagtaaag
     4981 atggagagat agtacttgac acatattctc taaacaattt aaatgtaaac aaagaaattg
     5041 gaacagaaat caatttagat ggtaaaatct ataagttatg tggtatttta tcaagtcttt
     5101 caaacccaga aactgctctg gagggatttg taaacgatta ttttcagcct aaagaaaagg
     5161 attcttatat agtttatgta aaatatgatg aaaataaaaa tattaaaaag caaaattata
     5221 aatttatgaa aaaattcaac atatcaaaag aaaaaactta tataaactgg cctctttcag
     5281 ttgaatttgg agtaaaacca ccaaaaaata tagaaaaata taatatattt gagttcttaa
     5341 aaaatttaga actaaataca aatgcaatta tattacttgt tggagtgttt ggtgcagcag
     5401 cagtatatag tattttccat gtttcaatac ttcagagaat atcacaatat ggggttttag
     5461 aagtattagg cgctaataat aaacaacttt tatttctact atttttagag ttatttttac
     5521 tttttattgt tggatttcct ataggttgct tcttaggtat tgaagtggcg tctacaatat
     5581 atgaacaatt tccacatata tttttgagta gtgatattgt tccaggagca ttttttataa
     5641 gtgaaaaatc tatttattta ggctttttat ttatcttatt gttgttaatt ttaattgctg
     5701 tacgtgttgt acatcagttg aataaatact ctagtataga atctatgaaa aactttaaga
     5761 ttactaataa gaaaaagaga atattagaaa gtgtaaaaca ctctaacatg acaaaagtat
     5821 tatctcataa atacatgact caaaaaaagg gaatgtttat tggaattttg ttttcattat
     5881 cacttggtgg aattatattt ttatgttcta gttactctat acagcttact aaaaataata
     5941 atgaacttac aatgaaagct gatgatggtt taaattctga ctaccaaata actatgcaaa
     6001 caacagattt tgatgtaggt ataccaaaag ataaaatttc aagattaaac aatgttgagg
     6061 gaatatcaaa agtctatcca gtaagatatt tatttggagc tatgcaaatt aaagaagaac
     6121 aactattttg gaaaaatttc tttaaacctc ttgaaaaaga ttacaggata aagacttttt
     6181 ttgatggtat ttgtacaaag caaaatgaag gtggctatct tttaaaaaca aatatttatg
     6241 gttataacaa tggtatgtta gaaggattaa attcttatat tatagatggg gagattaata
     6301 cccatgatat ggttaaaaat aataaggtta tagttagact acctatggat ggaactggga
     6361 tgtatgatgc agttgatata aatccaggag atacaataaa agttaaagtg ccaaagacaa
     6421 tgaaaccaac agatgaaatt gttaaattta aagaagaaaa taagaatgat tatacaacaa
     6481 aagaatttgt agttgctgct actgtaaaac gtgtgatggc aaataacata tatttcattg
     6541 gtgattatgg tatggatata gttatgacga atgggcaaat gaaaaataac tttgatatcg
     6601 aaaattataa ttcagtaagt ataaaaaaga cagaagatag taacagtgag gaattgtctg
     6661 agaacataaa aagtgttgta agtgatgtaa aaagatgtat cgtaacagat tataccattg
     6721 ctattgaaaa aaataatacc tacttaaatc aaaaactctt atttatatat ggtattgtat
     6781 ttgtacttct tgcaataagt ttgttccata taatcaatac agtaagctac ttaatttttt
     6841 caagaagaca tgagtttgga attttaagag ctatgggtat tacggataat aagtttttac
     6901 ttatgatgat aagagaaggt tttttatatg gattctatgc aagtattata atggtaattg
     6961 gaagtgttat tggacaattt atgatttatt ttatggtaaa acgtgtatat ttatatataa
     7021 atccaattct taaaataaat acacctctat acataggtat gattatttta aatatcacta
     7081 ttagtatagt tgctgtaatt attccagtaa gacaaatttt gaagagtgat attatttcag
     7141 aaataaataa gaattagagt tatataaatt tttaagatta tataactttc tgacaatctt
     7201 gtttaaatta tttaaatata tcttatctgt gaacattaaa taaacctctt aatagatata
     7261 actcttgaaa agtcatatct attaagaggt tttaataaat tttcatgtta atatatataa
     7321 tccaaaaaaa ttaaatctta ttaaaacata ctattttgta tagttatcga aatatccttg
     7381 tatgtaaata ataggagtac ccttatctcc acttccagat gttaaatctg ctaaagaacc
     7441 tataaggtct gttaatcttc ttggagtagt accttgagaa accatgtttc ctgttaaatc
     7501 atcagattta ttatcttttt caactatgta ttttgaaatt gcttctttta actcatcacc
     7561 agataagtca gcaaaatcat tatctgctaa gtattttaat tttacttcgt ttggagttcc
     7621 ctctagccct tttgtataag caggagatac tactgggtca gctaattccc atattttacc
     7681 tacagggtct ttaaaagctc catctccata aaccattact tctacatctt tccctgttat
     7741 ttcttttata ttaccttgga ttttgtttac aacttcatca caatttatag ggaataattt
     7801 tactgtttct tctgtagctt tgtttgaacc taaaagtcca tactggtcat tgtaaccact
     7861 accatctaca cttgctgtca ttatatcatc aagtgaatat acttttttag ctccattttg
     7921 tcttaaaatt ctctttgttc tttgtcttgt atgtatatca caagttaaaa tacttgttgt
     7981 atagttaaga atagtttttg gattgtttgc aaatactatt tctacctctg caccacaatc
     8041 cttgattaaa ttcttataat aatcaacata atctacacca gtaaatgtat gcttgttata
     8101 tccaaatagt tctctgtatt tttcttctgt taaaacatct gaccaaggat ttattccctt
     8161 atcatcaagt tcatcaatac ttattaagtg atttccaact tcatcagaag ggtagcttaa
     8221 cattaatacg acttttctac atccttttgc aattcctttt aaacatatag caaaacgatt
     8281 tctacttaaa attgggaata taactccaac agtatcatct ccaaatttat cttttacatc
     8341 ttttgcaata ttgtcaacgt gagcataatt tccctgtgct ctagctacaa cagcttctgt
     8401 aacggcaatt acatctttgt ccctaacttc aaagttttca ctttttgacg catttaaaac
     8461 agaatcaaca actattttaa ctaagtcatc tccttgtctt ataataggtg ctctaacccc
     8521 tcttgaaaca gttccaacta atctatccat tatctaaaat cctcctaaag aattaatatt
     8581 gtcatattag taccatgtat attataattc aaatataatt ataagtaaaa taaataatac
     8641 taatatttga cataaggagt aattatatat gaatataaaa cttgaacttt ataaggtttt
     8701 taatgcagtt gtaaataata agagtttttc tcttgctgct aaggaattgt ttatgtcaca
     8761 gccagctgta agccagtcaa taaagcaatt agaagaacaa ttagatactt tattatttta
     8821 tagaaacaat aaaggagtaa aattaactcc agaggggaaa attctaagtg aacatgtcac
     8881 tactgcatta aagttaatat catcaggaga agatagaata aataaattta aaaaattaga
     8941 atacggttct ctgaaaatag gagtaggaga tacagctgca cgttttttct tgttaaaata
     9001 tttagaaatt tttcataaga agtatccaca tatacatgta tctacaataa atagaacgag
     9061 tagagaactt atatctttac tcaaagatgg gaatatagat atagcaatta taaacatgcc
     9121 aatagaa
//
LOCUS       DAGSHT010000137         9068 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.141, whole genome shotgun sequence.
ACCESSION   DAGSHT010000137 DAGSHT010000000
VERSION     DAGSHT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.141"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..598)
                     /locus_tag="MJB72_002710"
     CDS             complement(<1..598)
                     /locus_tag="MJB72_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBY8263792.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIK"
     gene            complement(670..1023)
                     /locus_tag="MJB72_002711"
     CDS             complement(670..1023)
                     /locus_tag="MJB72_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBY8263793.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1101..1304)
                     /locus_tag="MJB72_002712"
                     /pseudo
     CDS             complement(1101..1304)
                     /locus_tag="MJB72_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            complement(1694..1924)
                     /locus_tag="MJB72_002713"
     CDS             complement(1694..1924)
                     /locus_tag="MJB72_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8263794.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1926..2333)
                     /locus_tag="MJB72_002714"
     CDS             complement(1926..2333)
                     /locus_tag="MJB72_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8263795.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2694..3132)
                     /locus_tag="MJB72_002715"
                     /pseudo
     CDS             complement(2694..3132)
                     /locus_tag="MJB72_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3146..4072)
                     /locus_tag="MJB72_002716"
     CDS             complement(3146..4072)
                     /locus_tag="MJB72_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263796.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4197..4979)
                     /locus_tag="MJB72_002717"
     CDS             complement(4197..4979)
                     /locus_tag="MJB72_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBY8263797.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5135..5755)
                     /locus_tag="MJB72_002718"
     CDS             complement(5135..5755)
                     /locus_tag="MJB72_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY8263798.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5882..6550)
                     /locus_tag="MJB72_002719"
                     /pseudo
     CDS             complement(5882..6550)
                     /locus_tag="MJB72_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6642..7457
                     /locus_tag="MJB72_002720"
                     /pseudo
     CDS             6642..7457
                     /locus_tag="MJB72_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7468..7617
                     /locus_tag="MJB72_002721"
     CDS             7468..7617
                     /locus_tag="MJB72_002721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263799.1"
                     /translation="MTRVYYLFTTFDGILIRLNANLCKVSSKKKMSILLVDTGYIDIY
                     QLMKR"
     gene            8128..8895
                     /locus_tag="MJB72_002722"
     CDS             8128..8895
                     /locus_tag="MJB72_002722"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBY8263800.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         3040 a         1452 c         1371 g         3205 t
ORIGIN      
        1 gcttaatgtt tgattgaatt tttttatatg gccaatccca ccatttaatt tctaataatt
       61 ttgaaattgt atcattatta aatctctttt ttataatttt tgcaggtact cctccaacaa
      121 tagaatacgg tggaacgtca ttagtaacta ctgctcttgt tccaataatt gcaccatcac
      181 caatatttac acctgacata attatagaat cataacctat ccacacatca tttccaatta
      241 taatgttacc cttattgtcc caagcgtccg ttatatggtt gatatcgagt ttccattctt
      301 cataaaagat gggaaaagta tatgtggatt ttgatttcat tgagtgattt ccactagtca
      361 ttagaaattt tgcattacat gctattgagc aaaacttacc aattattaat ttatcattgt
      421 ttatagggta atgatacaat acattgtttt tctcaaaatc tcttgggtca ttataaaagt
      481 cattatatat tgtgtaatct ccaacttcta tattttccct tgttattaca tttttaagat
      541 atacagtttg ataatcatta cttcttggat ataccttatt tgaacttgga atagtcatat
      601 ttaaatctct cctttaatat atattatgtt tttgactatt ccactactac aatacatctc
      661 ttcacagaat cactccttgt taagaatctt cataagataa tggcgtcctt ttttagtgtt
      721 aagtcggata ttctttctta gtacattctt tttagcgtat aactgacaaa ctgtcatttt
      781 cttaccaata gtgtcaatgc tatcatcaag gtcattttgt attgcttttt ccttatccct
      841 taatgatata tcatcacgtt ttccctttgg gggtttatca gtaggtacaa gtttccaaga
      901 gtaaacaaat tgtgctttac caaatgaata tacatactta tatgcgtatc ttccatcttt
      961 tctctggctc tctccagttc ttaaaattct atctttatta tctcgtcttt tttcagttga
     1021 catgataaga agctcctctc aataaccgaa ttgagcttga tacgtcatat gaatattata
     1081 acatataagg ctcaatttca ttagataacg tcaagtgtat caacgatttt ctcaaattgt
     1141 tttctcttta tttgaatgcg attgctattc ataatgaccc agccagaatc aaggttttca
     1201 tcagcaagtt tacgtaattt tttctctcca atacgaaagt attttgacgc ttattcaatc
     1261 gtcaaggtat atttttccca aataggtaca tcagtattgt tcatagaatg tatcactcct
     1321 ttctatgttc gtagttcaga agtagataga tggctttggt aagctccatt ggtatttcaa
     1381 ccaatcccat tcttatgggc agaaccgtgt catacaggtg tatcattata ctgtggtaca
     1441 ggttacggca aaacaaccgt tccaaaggtt attcacaaat cactatatag tagctctctt
     1501 gattaaggtc atggcgtata gtatttgttg gctatctgta tcacaaacgt atctatctac
     1561 tttattcagt tgtcaaagaa cagtcggcat tgatttgcct ataaaaaatc acaatagctt
     1621 atgggctttt atagacaaat aattagtgat tctccatgta ccaaatagct tgatacatgg
     1681 agagagggcg agattaagac aaattaaatc tcaaaatagc agtgattagt tttgttccta
     1741 gcattcgctt tgtttcttct tctacatgaa tatagggatt tccgtattca tcaaataaag
     1801 ttttctgtga aagacgaatg atatagtgtt caaaatgttt aagtattacg tttattgcat
     1861 ttacatcgcc atgtacagca agtaaaataa ttgtaaaagg taatacttct ttagtaggtt
     1921 tcatttcaat gttcctgcat gagttctttc aactctttta gtgcatttgt tttgtgataa
     1981 tgtatggtat tttctgctaa atccatcaat gttgctatgt cagcatttgt catgttgaga
     2041 aagaatgaaa gtagaattat atcttgtttt ctttttgtaa gttttccgat tgcttgtgcc
     2101 attaatacat cttcaattcg tatgttgtag tcgtaagaaa ccatacagat gtagttagaa
     2161 tcataatcat ctaaaatact caatttactt aattctgcaa gtgacaaaga ttcgagagat
     2221 accaaataat catttctgcg tttattttcg tcataaatat ctctagcata gttacgcaaa
     2281 actgttttac agaaactgtc gaattgatgt tcaattgtct tttcatcaac cataagattt
     2341 caccctcttt ccatcgcaag gagttaaata accttgctta ctcctatcac ggtgggaggg
     2401 cgttaaatct tcgataaatt tcaaaaaaaa agagaaaaaa atacacactc tgcctatagg
     2461 gcaaaaatgt gtattcttta aaacgtattt aatttatatt ggttgtaagc caccaccata
     2521 ttgatgtatg gaaatatgca ttggctagct tttcataaaa atacaaagat gcataagaaa
     2581 ttaatagagg ggaaaaactt attctgttat taaaaagtag gttaattcta ataaatgcta
     2641 ttgcggtcga attaaactat atacatggca atcaatcatg tttctattct ttattatgaa
     2701 ttgtgacagt attcttcaat cttaaagcca cattttactt gactctctat tgtaggaata
     2761 tacattagca tgaattttgt taaggttgta tttttcaaag gcaattttgc atatttcttt
     2821 tactgcattt gttgctatcc ctttgcccca atagttccta gcaagtcagt aacctaattc
     2881 agcattttta ttaatagagg tatcttcttc aaatgttagg ctgatacttc caatagccct
     2941 ttcttgataa aaaatagcat agctaagttg tttaacatct gaagttgtca aacaaaaact
     3001 aataaaatct ctagcgtttt gctacctata agggtggggg aagttgtcac gaagattgtt
     3061 tgcaatttct ttatcattcg caaactcaac taaatcggta atataagcat aattccactc
     3121 tataagttcc atttttcttc tccttttatg aaacagctac aagaaatctt tctaagccaa
     3181 taccacttat caaacagcgt tcttttttat ttccaagcat ttgataagtc cagtcaacaa
     3241 atccaccgtc aactatttca acggtttcat ttccttgttt gaccgataat ttgaaattga
     3301 tacctttata ataattatta tcaacatcat cattatgaat tgataactct atattaggaa
     3361 aaataagttt gatagtttca accattctgt cgaaaaatcc gtcattatct ttgtatccgt
     3421 ttcttttgcg tagaataata gagaaacgaa cattttctat ttcacttaat atatctttat
     3481 aatagtttaa gtgcttttct aacaacattt tttcacaagt gtaagaccct gtatctatac
     3541 cagatgaaac catacaatat aagccaaaat gtgaatataa acctttacct acaaaatttt
     3601 gtgctcttat taccctagca gtagtagcca tgtggatagg tatggtatta ttttcttctt
     3661 ttctttttaa tttatcagca ataattattg ccaacatatt tgaggggtct gcaagtgttt
     3721 ctgtccctcg tagagcactt actacattat attgatctac ggagccaaaa acgctacagc
     3781 tacctaatgg agcagaggga gaaagcataa tcgtcttgat atccatattg aatgcttttc
     3841 tcaataattt tgactccaaa gcatgaattt cttctggatc tataccgcta gggatagtaa
     3901 atctatttag ttggtatgac tttagaatat cagttgcagt taaagtattt gcttgtctat
     3961 caaacacttc taatagaagt gagttcaaat ctgattttga tagagctgat aacttatcaa
     4021 ctatattctt atccccaatt ttatttgtaa ttctacttaa aatatcactc atagttatcc
     4081 tccatactgt tactttcaaa ataattatag aattgttaca acaactaatc taggtcattt
     4141 gaccaaaaat gaaaaaacac tgaatattta ctattcaata tttttttact ttgggtttat
     4201 ttgattaaat cttgaaaata ctgtaatgca accctttcag cttcaacctt tcggacaata
     4261 ggactttgtg ataattcctc tcttaattca agatacaggt tgatagtagc ctgcaatgca
     4321 ttaacacagc ttttatattc atggttagca accgtctttt ctaatgcttc aatatattgg
     4381 ggtgcgtcca attcaatttt tctaactcca cgaggtaatt tacctaagag aagctgtatt
     4441 aacgaggcaa gtactgattg tcttaaaaaa gataaaaaat caattgtttc aaatagttct
     4501 ctacgtccaa tcttagtgca agtgtaatga acccaaatcc aaaatctatc ttcaatccac
     4561 tgtaagtctg gagttgggaa gtatgcttct tttttagaat aaatttctgt tatgcagttg
     4621 ttttcttcat agagaatagc tgaatcttca accctatcta gtaaaccatc taaggttgta
     4681 aacttaaaat caacatgaag aacaggggaa tcatagaggg aaattatcaa tcgaggttct
     4741 ccaacatgtt cgccagtaaa agcagaaact aatgttccaa actgttccac aataagtaat
     4801 ctctcttcga taacttcttc ataggattta tcatctgcta caatcagaaa atccaaatca
     4861 gaatattcat ctaacgattt tgtgatataa gaaccgctaa ttgcaagtcc tattatccta
     4921 ctatctcttt ttgaaaattc taatacgttt tgtatcattt cctgttgtgt tttagtcata
     4981 atataacacc tctcactgtc ttttattagc tatcaatcca acataattat gcataggagt
     5041 attatataat gaagaaataa aaaaaaatag gtcacttgac ccgaaataaa aaagtattga
     5101 ataattatta tccaatactt ttttgaaatt atacttatgt tgtaggaaga ttctttaata
     5161 cctctcgctg attaattaca ctgaatcttt tgttttcata agcaattaga tgttcttgta
     5221 cacattcatt tatagcacga ttaatgcttc taatcggagc gtttacttcg ctagcaagct
     5281 gttttttggt aagcagattt aattcacttc tataatagtg taaggatata cacctcagaa
     5341 gtcgttcttt aacacttaaa gatgataatt cttcaattaa ttcagcattt acaactaatt
     5401 tactagatat ttctcgaatc agaaattttg tagcttcaaa atctcccctt aaccagttta
     5461 aaaaatcatc tctatgaagt ttatatacat tacaatctgt gaaagcgaca atttcaacgg
     5521 gttttttccc atcattaaat tgttccattt ctccaaaaaa actaccactt ctataaagat
     5581 gaatagttga gatgtttcct ttcacattta agatgaatgc ttctgcaata ccgtcaatca
     5641 taagaattac atattcattt tcttgctcag caaaaagaat agtctctttc tttttatacg
     5701 tttttttaac aagcttcttt ttaatatctt cattcataaa atcaattaaa tgcatattca
     5761 tctctcctta ccaaatcttt gattaaatta tatcatcttt caattagtta ggtaagatag
     5821 taaaaatata tttacataga aactattcca ttattatttt ccagttgtca tttttctgca
     5881 aaaacttatc actgtcacga ctggagatac cagtaaggaa tgttaggata tcaatcgcta
     5941 aactttcaga atccttttta cggctcaaaa tgacgtaatg gtcaagcaat cctttgtttt
     6001 catcatcact ggttaaattg acaatgttta tctcatgggc tatttctggt agcgtttctt
     6061 tccatttgcc gcgttttgct ttagggtcaa ctatgaccgc ttgtccgccg aatagtaccg
     6121 catagtaaac aagcatattg ttactgaata atttaccacc acccaatgaa ccgataaacg
     6181 cagacgctaa cgcattggta atagaaccct ttaccccttg actagcaagg gcaggtttta
     6241 gatagacatt ctttccagtg tccagattgt agccaagata gatatcctca tgttcgccaa
     6301 gggtttgtgt cgctccaaaa ccaagtccag ccaaaaaatc cgaagtgaca tattgaatat
     6361 aatcattgat gtaacgtttg ctgacaggaa taaattcgcc atgcaatccc aacatatccc
     6421 caaagggacg cacgagcttg attgataagt cgtcgtaaaa gtctttgact tcatcacaac
     6481 gcctttttag ctcgtcaaga tcctttgtag atacacggac aacatagcta agtttataca
     6541 tagattcttt acagttaaaa gtctgttttt aaaaaaatgt ataaattata aatcaaaata
     6601 tagatactat caatgaccca ctaggtgcaa aaagcattga tatggttaaa ttatcagacg
     6661 ctaaaagctg ggctatccga atgagtgaag atgggtgtgc ttataaatcc attgacaatt
     6721 ataagcgctc actgaaagcg tcgtttttca tggcaattca agacgactgt attagaaaga
     6781 atccatttaa tttttagtta agtgatgttt tggaagatac aacagaacca aaaatcgctc
     6841 taacaccaga acaggaaatg ggcttattat cctttatggg gagcgataag acttattata
     6901 aatattatga tgatgttgtt atcctcttag aaacaggact acgcatttct gaattatgcg
     6961 gtttgacttt agaattagat atgaaaaata aagccattat ggtagaccat caattgttga
     7021 gtgatactaa gattggctat tatgtagaag ttccaaagac cacacagggt aagcgagaaa
     7081 ttccaatgac tgaaagagct tatcaatcat ttgaacgtat cgttgaacgg cgtaagaacg
     7141 ctaaagcaga accaatcatt gttgatggtt accagaactt cctatttctt aaacaagatg
     7201 gattaccaaa acaagcaact cattatcaaa gcatgttaag agcgttagtc aagaagtata
     7261 acaagaccca tgaggacaag ctaccaaaga ttacgccaca tacctttagg catacactct
     7321 gtacaaatat ggctaacaaa ggaatgagta ctaatatatt gcaatatatt atggggcata
     7381 aaaacattac tatgacgctt ggctattatg cacatggttc atttagttct gcgagagctg
     7441 aaatggaacg gttataatcc tagtagtatg actagagttt actacttatt tactactttt
     7501 gatggcattc ttatacggtt aaatgccaac ttatgcaaag tatcttccaa aaagaaaatg
     7561 tcgatactcc ttgtagatac aggatatatc gacatttacc aacttatgaa aagataatca
     7621 aatttaatat aagtttttca aataaaaaga tactaaattc tttttagaga atctatataa
     7681 cattacttaa gcttggttgg ggtataaaca attgagtagc tacttttgta tggtgttcaa
     7741 ctttagtcaa aactcttaaa taatgcaaat gatttagatt cattaatatt caatctcttc
     7801 ataagtagtg aaattcgagt tatttctgca taattatact atgatttata gattaattct
     7861 attagcatcc agaataatat tgtttatatg agtaaaattt aaagaatatt gtgaaaatta
     7921 tagaatgtta aaataaattt aggtaaaaaa aagtgtttgt atattataga gtaccttttc
     7981 taaaatatgt gggattgttt taagaattag aataaaaatt atttttaaat tagtattttt
     8041 ataatgtata gtatttttaa tgtataatta aattgtgcag aaaatagaat ttaaagtcct
     8101 atagaatttc tgattatgga ggagaatatg aaaagaaaag ttcaagtaaa aaatattact
     8161 attggagaag gtagacctaa aatttgtgta ccaattattg ggaaaaataa aaaagatatt
     8221 ataaaagagg ctaaagagtt aaaagatgca tgtttggata ttatagagtg gcgagttgat
     8281 ttttttgaga atgtagaaaa tattaaagaa gttaaggaag ttctctatga attaagaagt
     8341 tatatacatg atatcccttt attattcaca tttagaagtg tagtagaagg gggagaaaaa
     8401 ttaatatcaa gagattatta tactactttg aataaagaaa tttctaatac aggtttagta
     8461 gatttaatag atgtagagtt atttatggga gatgaggtca tagatgaagt agttaatttt
     8521 gctcataaaa aagaagtgaa agtaattata tctaatcatg attttaataa aactccaaaa
     8581 aaagaagaga ttgtatctcg tttatgtaga atgcaggaat tgggcgcaga tttacccaaa
     8641 atagctgtta tgccgcaaaa tgaaaaagat gtgttagtgc tacttgaagc tacaaatgaa
     8701 atgtttaaaa tttatgcaga tagaccaata ataacaatgt caatgtcagg aatgggtgtt
     8761 ataagtagat tgtgtggtga aatatttggt tctgcattga cttttggagc tgcaaaaagt
     8821 gtttcagcac caggacaaat atcatttaaa gagttaaatt cagtacttaa tctattacat
     8881 aagagtatta attaaattaa ttatatagaa atattagaag gtattttatt tttgaagata
     8941 taaattaaat ataaaaaact tttcagatag taaaatttaa attgggcaat tatatctgaa
     9001 aagcaactat ttagtgtgta ttttgaaagt gatatagaaa atcttataaa tcaactatgg
     9061 catattta
//
LOCUS       DAGSHT010000138         8847 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGSHT010000138 DAGSHT010000000
VERSION     DAGSHT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.143"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..314)
                     /locus_tag="MJB72_002723"
     CDS             complement(<1..314)
                     /locus_tag="MJB72_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263801.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     IT"
     gene            complement(692..892)
                     /locus_tag="MJB72_002724"
     CDS             complement(692..892)
                     /locus_tag="MJB72_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263802.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILEDIEIDLI
                     IIDEKNNVVWRNEKNTPAHENA"
     gene            complement(911..1324)
                     /locus_tag="MJB72_002725"
     CDS             complement(911..1324)
                     /locus_tag="MJB72_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263803.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(1308..1541)
                     /locus_tag="MJB72_002726"
     CDS             complement(1308..1541)
                     /locus_tag="MJB72_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263804.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1760..2134
                     /locus_tag="MJB72_002727"
     CDS             1760..2134
                     /locus_tag="MJB72_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8263805.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            2424..2942
                     /locus_tag="MJB72_002728"
                     /pseudo
     CDS             2424..2942
                     /locus_tag="MJB72_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3374..3628
                     /locus_tag="MJB72_002729"
     CDS             3374..3628
                     /locus_tag="MJB72_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263806.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            3664..3987
                     /locus_tag="MJB72_002730"
     CDS             3664..3987
                     /locus_tag="MJB72_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263807.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            4261..4413
                     /locus_tag="MJB72_002731"
     CDS             4261..4413
                     /locus_tag="MJB72_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263808.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            4607..5530
                     /locus_tag="MJB72_002732"
     CDS             4607..5530
                     /locus_tag="MJB72_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBY8263809.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   5797..6811
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5797..5825
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            7002..7154
                     /locus_tag="MJB72_002733"
     CDS             7002..7154
                     /locus_tag="MJB72_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263810.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            7275..8088
                     /locus_tag="MJB72_002734"
                     /pseudo
     CDS             7275..8088
                     /locus_tag="MJB72_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            8140..8301
                     /locus_tag="MJB72_002735"
     CDS             8140..8301
                     /locus_tag="MJB72_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263811.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(8630..>8847)
                     /locus_tag="MJB72_002736"
     CDS             complement(8630..>8847)
                     /locus_tag="MJB72_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263812.1"
                     /translation="LTFDLSDITNYFIRMIMTLLFIVLLIFLVNKGILPIAIPYRNNI
                     QFYTTALDVLEDMKKKILRERKYKKRR"
BASE COUNT         3601 a          913 c         1417 g         2916 t
ORIGIN      
        1 atagttatat atttctttct aattagataa tataatgctg ttgttataat ggctactaat
       61 attattgcta gtattgtaat atctaatctt cttgtaaata agcctattgc tatatactca
      121 agacctgtta cataacgata aagtacttct agtttaacag tttttttctt atctatattt
      181 ccatatattg attctaattt attaaaatat tttttactac aaaaagcagt tttaggttta
      241 attaaatata ttattgtagt tcctattata actattattc caataagtat aagattgata
      301 tttccaaaat ccatagcaga tacctcctat taaatttgct tttcttagtc aaagtatatc
      361 agtaacacaa gtacaaagag tatataaaat attacaaata ttaacaggat agataaaact
      421 atagtaaaaa ttgtaataat aaagcgaacg aaaattatca tactaaaaca taagacatgt
      481 tataattgta ttagatagaa gattaacata caccaaagca ctccttataa taaagagtgc
      541 ttattttttt gaaattcatc aatatataaa ctatcaagaa tcttattcaa cacaccttaa
      601 aatcgattta aatagctctt tttgatagca taatttatat gatataataa aaaaatagaa
      661 gtgtattcgc agtacacttc tatagttata actaagcatt ctcatgagcg ggagtgtttt
      721 tttcatttct ccaaacaaca ttattttttt catcaataat tatcaaatca atttcaatat
      781 cttctaaaat ttctgttgca gtacaaatta gattatctaa gtcaaaactt cgagttatct
      841 ctagttgttt gttatttact tctcttttta gtatataagt tttaaaatac atttatatca
      901 ccccttgtat ttactctttt ttctctaaaa atatattgat aagtaaatat gcaacaaata
      961 aaaaaatctc aatgacacat agtgttatag ctataactag aagtgattga tttttccatg
     1021 cactataagc tatgatgaaa agaaatgtaa aagtaaataa tgtataattc ataattttaa
     1081 ctaagatatg acttgttttc attgttaagt acaagtctct ttcatctgca ctatcgtgta
     1141 attcttcaac aatgcctttt cttgaaaaag cattacaaaa attaaagatt gctattacaa
     1201 ttaaaagaaa tgatgaaatt aggaatcttt tatcttttaa aatgcaataa ataactaaac
     1261 tagcacaaca taacatacaa actataatac ccactataaa acttcgttta ctctttactt
     1321 tcatgttttt tatcctccat ttctttattt tcttttaaac aacatagttc ttcaatcgtt
     1381 gtaccaaaca cttgtgccat acgataggca agcataagag aagggctata ttgctctttt
     1441 tcaatagaaa taattgttct gtttgataca tggactaaat cagcgagttg ttgctgtgtt
     1501 agacccatat ttgttcgaaa ttctcgtact tttgttttca ttccataacc tctttctata
     1561 tgaagctaac ttcatgtgaa gttaacttca taatatccta aaatgagtaa aatgtcaatc
     1621 tcattagata agtattataa aacctaaaag aataatatgg taaaaattgg atggaattat
     1681 ttaccttaat tctcttgttt gtaaaacttt attgatatag aatttaacat atacataaat
     1741 cggcaaaggg gtgcgaatta tgaaaattag taaattacca gaagcggaat taaaagttat
     1801 gagatatatt tgtgaagcta acaaggtatt aacttctagg gaattagttg ctgcaatgga
     1861 agaaaaatat gagtggaatg aatcaactac atttacagtt ttgaaaagat tagagcgaag
     1921 ggagttttta agtacagaga aaataggcaa acttacacat tataatatac tagtaaaaga
     1981 gaaaaaatat ctaaggtttg aaacaaagga atttttgaag aacatacata aaaattcaat
     2041 ttcaagttta atgtcagcgt tacatggtga agacgacgaa gtggatgaag ataaattaag
     2101 tgaactcgaa gaatatttta agaatttgaa ataagaaaat gacaaattga tacataataa
     2161 ataaaatgaa ggtatgaaat tataactttt tctaccttca ttttgtttat tattctgaac
     2221 atgttaagaa aatgaaataa gtttgtattt tatttatata aattttaata gaatatgtat
     2281 agtatatagt agatagaatg tataaaaata aaaactgttc aataagaatt aatatacatt
     2341 aattcagctg aatccaatca aaatacttaa ataaaagtaa tctgaaaata acataattca
     2401 aatcaattta ttcaaataaa cctcaattta tctcaaatct taagaagtca ttaaacaaac
     2461 ttgctaaatt acaaagagaa ttatcaagaa aaacaatcgg tagtttaaat agaaataaag
     2521 caagattaaa agttgcaaga cttcaagaac atatagctaa tcaaagaaat gattttctac
     2581 aaaaactatc tactaaatta ataaaagaga acgatattat ttgcatagaa gatttacaag
     2641 taaagaatat gattaggaat cgtaaactat ctcgctcaat ttctgatgtg tcatggtcag
     2701 agtttattcg tcaattagaa tacaaggcta attggcatgg tagacaaatt gtaaaggtgg
     2761 gtaaattctt tgcaagttca caaatatgca ataaatgtgg gtataaaaac gaggaagtga
     2821 aggacttaaa tataagagaa tggatttatc ctagttgtaa tgaaactcac gatagagata
     2881 taaatgcaag tataaatata ttaaaagaag gattaagact aataacaatt caaaataaat
     2941 aacgtatgta agaaccgtag gaactacggg gatagcctgt ggagaattag taagacatat
     3001 gttagtatgc aaaattctgt gaagcaggaa acctgtgact ttagtcatgg gaggttcagg
     3061 ctagtattac tcattattgt attcaaaagc taaagtggaa acctagtgaa tatatgaatt
     3121 tagaagttaa tgagagagca ttagcagccg actcaatact tattaaaatt gaagatgaag
     3181 aagaaacaat gaaagaagct gaaagagaga aaaagagagg tagaagaagg tagaatttaa
     3241 agttaaaata gttacattgc taatatatga aaatgttaca aatttggaaa tagatggata
     3301 tatatgttgt atagtataat gtagataata tatcaccaag taaaaaacaa caaataaatc
     3361 taaattatga ggggtggaaa ataaagatat taaacttaca atatgtatat ttgcttttgt
     3421 aattggaata atagtgttat tttgctgtat aggtctaggt caatttaata tggattgtat
     3481 tataaaacaa aatggtgggt caatagctac cgaacaatac aacatatatt tagaacaatc
     3541 tatttcacaa tatagaaatt ttggttctat actggtttta ctaggaggaa taggaatact
     3601 gtttgataaa agtgtcagaa aatactaaaa aagtatattt ttatttagga tgtgatgaat
     3661 gatatggcaa gagatgtatt tagtgtaaag atgacaccac tagaagcaca gaaaatggta
     3721 gaaaaaaatt tggaagcaga tttagtttct gttgatattt ataacctaga aaataataag
     3781 tctataatag ttacaatctt tgaaaaatat tatgcaagaa gcaatagtga tgcaggtttg
     3841 gtagtgattt gtgaaaatac tacaggaaaa acacttgtta aaattacctc aactggtagt
     3901 gcaattggac tatttcagat tgattgggga acaggtaata atttgattaa aagagtaaaa
     3961 aaaatactta ttgattatat tatttaatta ttttatgtag atattcttag gttggtatgg
     4021 aatttgtact aacctattaa gtaatcaata aaactaaata aacatagaaa gcacttacaa
     4081 atatgtaggt gctttttatt gaaaaaatag aaaataaaat aaaaagtaag atataggaaa
     4141 aatatgtaag aattatatgt tataataatt gtagcaagaa gatgtaatct acaatttata
     4201 gagtggagtt catgtattaa aaaattatcc tcccaacgat aagaagggag gtgaatatac
     4261 atggataatt ttttatttaa tgtgttagct agtgtaacag ctagttacat agtttatttg
     4321 attagcaagt taatcaaaaa agtaaaaagc cactctaccg caaagagtga cttaaagatt
     4381 gatataaatt ttaaatatca tcgtaaaaaa taactttcag ttatagaact tcactctacc
     4441 gcaaatagat tgtagttctt cttgttttta ttataccaca aattagaaaa aatattgcat
     4501 ataatatttt ttatagtcaa taaaaatatg aaatttttta tacacaatga tgatgaatct
     4561 aactagatga ataaatttaa ttgtaaataa gaaagtactt acttaaatgg taggtgcttt
     4621 tgttttgcca aattatgcta taatattgag tgagatgagt aatttgtggg ggaattttat
     4681 aatgtggata ttagtaccaa taataacaat agtattactt ataatagctg tttcttctat
     4741 gcaatatatt ttagttatga tagctttttt gttgattata tattctttta tagaaaagaa
     4801 aatagttatg ggtcttgtgt cagttctttt tttcacttat tcaatttatc tttgtgcaac
     4861 ttgggaagat aaatccttga tagcagataa taaagttgag actgttaggg cacaaagaga
     4921 aactgtagaa agagaaaaag aaatggaaag aagaagaata caagaagaag tagacaaaga
     4981 aagatatatt gaaaaacatg gaatggaaat atcagaaaat gatttaaaag ttaagctaga
     5041 ggctttagta cctcaagaat ataaggggaa gaaatatgaa ttaaaagtag gaaaatttaa
     5101 aagatatagt atgtattttg atttaactgt acaaaatgaa aaatttagta attctgaaga
     5161 atgtaaaaaa tttgtaaaag aaattgctaa tgatcttaaa aaaattaaaa taagcaaggc
     5221 gtactttaag tttcattcaa aagatgacgg cggtatatat aattctgttt atattgatta
     5281 ttttagaaac atacaaaata atgtagataa tgttgaaaat ttagaattca atgagtttga
     5341 acttaaaacc gaagaagaag agaagcgaga acaagagaaa atagaacaag aaaaaaacag
     5401 ttataataac tacattcaaa acagagtggt tgacccacta gatagaataa agaaactaaa
     5461 agagttgcta gattcaggag caattacaca ggaagaatat aataaaaaga aaaaagaatt
     5521 attagaatag ataatatagt aagcacttac aagtatgtag gtgcttttaa atttacaaag
     5581 tattccattt taattttata gtttagattt tatgatataa taaaaatata gaagttttgc
     5641 agtgtgcgat atttgttaca aagtagggct taatacttga aatctaagat gttgagggtg
     5701 cgtgataagt gttatcaatt gcactattgc ccgctcactg caattttaag agtattgtat
     5761 atatgtaggt attggaaatg ctaagtttat tttggggttt tagattaact atatggaatg
     5821 taaatattac atgggtaaaa attttgatga tttttatgac agttttagat taactatatg
     5881 gaatgtaaat tgtatagaat taagaatttt tttaatacta attatagttt tagattaact
     5941 atatggaatg taaatatttt tcaatatcaa tatagaactt tcatctttcc cgttttagat
     6001 taactatatg gaatgtaaat caagatattt ttaataaaat ttctgagcga actgtttgtt
     6061 ttagattaac tatatggaat gtaaatgagg agattcagac agcgactaaa aagactgata
     6121 taaagtttta gattaactat atggaatgta aataacttta tatctccatt tgcatatcca
     6181 tctgatgaac gttttagatt aactatatgg aatgtaaatg cgtacaaggt tgagaagctc
     6241 cgcaatctca acgtcgtgtt ttagattaac tatatggaat gtaaatattt gaaaggaata
     6301 aattttaaat gaatgaaagg ataggtttta gattaactat atggaatgta aatcagccaa
     6361 aaacttcgaa tttgaactaa aatgcgtggg ttttagatta actatatgga atgtaaattc
     6421 tcatgactat tgaataaata taataaacaa gcatacgttt tagattaact atatggaatg
     6481 taaatttaga atcaaaagca cttaatccta gttctttcat agttttagat taactatatg
     6541 gaatgtaaat agctttttag cttcgtctac tactttataa gcaaagcgtt ttagattaac
     6601 tatatggaat gtaaatgatg aacaataaaa caatcatcta aagacgaaga aagttttaga
     6661 ttaactatat ggaatgtaaa tgttcctgtt gtgtttttta ctaatcccat tttatatgtt
     6721 ttagattaac tatatggaat gtaaattgct gtgatttcac taataaaaca attaataatt
     6781 gtgttttaga ttaattatgt ggattcaaaa ttaaataaac aaagaaagca cttacaaata
     6841 tgtaggtgct tttattctgt tcaaaattgg tcggttgggt aaaataatta gaaaaaatta
     6901 gtaaaaacct cttttctgta actcgttaca atattattat taatgtaacg agttacagaa
     6961 aagaggtgaa taaaatagca actaaaagta gggcagagta tatgaaaaat cgtcgaaaag
     7021 ataaaagagg ttttagtgta cttttagaca aagaaaagtt agataaattt gatgaagtat
     7081 tagaggaaaa gaatctaacc aagaaagaat ggctagaaga aaaaatcgac gaggaactgg
     7141 aacaaaagga ataaaaaata agggtcactc ccaccgacca aagtttgagt aacccctatg
     7201 acgtatacta tcatatatca attatagtat atgtcattcc ttaaaaaaaa tcaattatta
     7261 aggagtgtaa tattatgaaa aatgaattaa tgatgtttga aggaaaagag attgaagtat
     7321 ttgaatttga aggaagaatt ttattcaact caaaagatgt agcaaactgt ttagacataa
     7381 aaaatgttaa tgagaatata agacttatga ataaaaagca agttgtaaag ttaagaaatt
     7441 cggatatcag taatactgac atccgaaaac ttaataatgc tggagaaaat tttcttactg
     7501 aaagtggagt gtataaatta atctttaaat caagaaaaga agaagctgag agattccaag
     7561 actggataag tgatgaagtt ttaccaagca taagacaaac tggtgcatac ataactaata
     7621 atgcaatccc gaaaagttga gagaaaaagc aagtgagatt gaaaaattac agttgcttat
     7681 aacagtacat ctatgttaaa agaattgtta gatgatgcag gctttgacaa taaatcaaaa
     7741 ctattaacag ctaaaacatt atataagaaa gcaggaattg atttaccaat agagataaac
     7801 gaagaagaac attattttga tacaaaacaa atagcatcta aactgaaaat atattctaag
     7861 agtaataaac cagctcagat ggctgtttgt gagattatta aaaagattga tttagaagat
     7921 ggtgaagtta aaggtgtttg ggaaactaat ggttcttgga ctggtactgt aaataaatat
     7981 acaaagagtg taatagataa ggtgagaact tggatagagg aaaataatag acctaccaag
     8041 attgcaggtg agaagaagaa ttatcatgta tcttacaaaa ttgagtaaat ttatcagttg
     8101 tattaaataa tttagtttag ttttgggggg attaatacaa tgtgtgagaa tttacttgat
     8161 aatatgtata ttgaaaagag aaaagaagaa tatagaatta aacttttgaa aataagagaa
     8221 acagatatag atatatataa taagttagaa agtatagtat ataaactttc tgagaaaaaa
     8281 ttagagaaaa ataataaata gacatataaa gcacttggat attctactgt ttcaagtgct
     8341 ttgtttggta taaaatggta taatggagat agagaattat attaacccaa aaatggagaa
     8401 aatagagaag atgatttagt tgacgcttta tcagtaggag tacttgcagg taagacagaa
     8461 tctctagtac tattagttgg aaatggaata agtgatactc aaaaaagttt aataaataaa
     8521 agagaattta gaaatataac tcaaattggt ggaaatggaa atgagaaagc atttagtgaa
     8581 gtagaaaact tagttaaata gattagaagg tagcaactag agttggttgt taccttcttt
     8641 ttttatactt tctctctcta agtattttct ttttcatatc ttctaataca tctaatgcag
     8701 tagtataaaa ttgtatatta tttctataag gtatagctat tggaagaatg cccttgttaa
     8761 ctaaaaaaat aagaagtaca ataaataaaa gtgtcataat cattctaata aaataattag
     8821 tgatatctga taaatcaaat gttaagt
//
LOCUS       DAGSHT010000139         8840 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGSHT010000139 DAGSHT010000000
VERSION     DAGSHT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.144"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            439..1347
                     /gene="trxB"
                     /locus_tag="MJB72_002737"
     CDS             439..1347
                     /gene="trxB"
                     /locus_tag="MJB72_002737"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBY8263813.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1437..3260)
                     /gene="typA"
                     /locus_tag="MJB72_002738"
     CDS             complement(1437..3260)
                     /gene="typA"
                     /locus_tag="MJB72_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBY8263814.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3472..3714
                     /locus_tag="MJB72_002739"
     CDS             3472..3714
                     /locus_tag="MJB72_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263815.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3862..4053
                     /locus_tag="MJB72_002740"
     CDS             3862..4053
                     /locus_tag="MJB72_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBY8263816.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4337..6838
                     /locus_tag="MJB72_002741"
     CDS             4337..6838
                     /locus_tag="MJB72_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBY8263817.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            6998..7666
                     /locus_tag="MJB72_002742"
     CDS             6998..7666
                     /locus_tag="MJB72_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263818.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7650..>8840
                     /locus_tag="MJB72_002743"
     CDS             7650..>8840
                     /locus_tag="MJB72_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBY8263819.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIV"
BASE COUNT         3464 a         1029 c         1527 g         2820 t
ORIGIN      
        1 aaaagtatat agttaataat gtttaatttg aagaaattgt tagtgtgaaa ttacatatat
       61 attcaaatta aattcataaa tatttcacat aataatagta tactgtaaac aaaaggaggt
      121 attaaatatg ggaatagtac ttataacagc agcattagtt ttggttatgt atggactttc
      181 aattctatca tatatatcag aaagcaatat attagaagac actgtaaatg ataaccaata
      241 aggttttgaa ttatataaat aagtgagaac tgtaaataaa agttaatttt tatttgcagt
      301 ttttttatat atatgtaaaa taaagttaat taataaaaaa taatgttttg tttatagtat
      361 atatggataa taatagtaaa gtataagaat gatgagaata atatataaga aataataata
      421 taaagagagg taaacgttat ggtagatatc attgtaattg gtgctggacc agcaggttta
      481 acttcagcaa tttatgctat gagagcaggg ttaagcgtta cagtatttga aaaaaatatt
      541 tatggaggtc aagttgcaag tacctctgag gtagaaaatt atccagcagt tcaaaagatt
      601 tctggggtag aattttctaa taatatatac aatcaagcag ttgctcaagg tgttgatatt
      661 cagtttgatg aggttgaaga gataaattta gagggaaaag taaaggttgt aaaaacttct
      721 tcaggtgaac ataaagcaaa ggctgttatt cttgcaaatg gagttgaaag acgtaaatta
      781 ggttgtgcag gggaacaaga atttacaggt agaggagtat cttactgtgc aacatgtgat
      841 ggagcctttt ttaaagataa agaagttgct atagtaggtg gaggaaatac tgcactagaa
      901 gatgctttat ttttagctaa taattgtaca aaagtgtatt taattcacag aagagatagt
      961 tttagaggtg aagaagtgtt ggagaaatct gtgaaagcaa gagagaatat tgagatactt
     1021 tacagtcatg gtgtagaaaa aatagaagga gaaaagactg tatcgaaaat agaagtaaaa
     1081 aacttaaaga ctgaagaaaa aagaactata gatgttagtg gtatttttat tgctataggt
     1141 cttaaaccta ataataaaat gtttgaaaat gttcttgatt tggatgaagg tggatatata
     1201 atttcagatg agtcttgtac aacatcagta gaaggtgtat atgtagcagg agatagtaga
     1261 actaagtttt tacgtcaaat aataacagca gcatcagatg gagctattgc agccgttcaa
     1321 gcagctaatt atataaatgt tgaataatat aaatttcaaa taaaaatagc catataaact
     1381 gaaataattt ttcagtacta tatggctatt ttgttaatta gaggtaaatg tacgttttat
     1441 ttaccttgtc tagatttgtt atatcttaat ctatcatgtt catttaagaa tctttttcta
     1501 agtcttatag aatcaggtgt aatttcaact aattcatcat cttctataaa ctctagtgct
     1561 tcttcaagag taaatattct aggaggagat aattttatag catcatctga accagaagca
     1621 cgtacgttac tcattttctt gtttttacaa ggattgacta ccatatcatc ttttctggaa
     1681 ttcataccaa ttatcatacc ttcgtaaact tctacaccag ggtctacaaa cattacagct
     1741 ctatcactta aggcattaag agaatacccc atagtaacac caggtccttg tgctattaaa
     1801 acaccatttc ctcttgaagg tatttctcct ttaaattctt caaatttttc aaatgaacga
     1861 actaaagtac cttctccacg agtagcgttt ataaattcac ttcgatatcc taaaagacca
     1921 cgagtaggtg ctaagaactc tatttttaca tagtctcctt ctattgacat agactccatc
     1981 ataccttttc tcatgtttaa ttcatttata atagtaccag agtaaacttc aggacagttt
     2041 acaacaactc tttctattgg ttccattagt tttccatctt ctttatgcat taaaacttca
     2101 ggtttagaaa caccaacttc ataaccttca cgtctcatat tttcaagtag tatagataag
     2161 tgaagctctc cacgtccaga aactttgtat ccatcagtag tatcaagagg ttcaacttta
     2221 agaccaacat ttacttcaag ttccttttca agtctatctt tcaaatgtct agttgtaacg
     2281 aattttccac tttttccaac gaatggagag tcgtttacta agaagttcat agaaagagta
     2341 ggctcttcta tttttatcat ttccataggt agtggactat ctaaatcgca tatagtttca
     2401 cctatagata tatcaggaat accagctata acaactattt ctccactagt agcttcatca
     2461 acttcaactt gttttaaacc ttcatacaca gaaagttttg atacttttcc tttagataca
     2521 acagaatctt ctctacatat agcaacttgt gtattgtttt taagagtacc tttgtaaatt
     2581 ctacctatac caagtctacc tacataatca tcatatgcaa gagctgatat ttggaactgt
     2641 agaggttcat tatcgtaatt aggataagct tcaacgtgat ttacaatagt tttaaataga
     2701 ggagaaaggt cttcactatc atcatccatt tcaagtttag caataccttg tttagcaata
     2761 ccatatatta taggaaattc gcattgctca tctgttgcat ttaagtctac aaataaatcg
     2821 aaaacttcat taactacttc ttccgctctc tggtcttttt tatcaatctt gtttataaat
     2881 aatattggtt ttaaaccgaa ctctaaagat ttttgtaaaa cgaatctagt ttgaggcatt
     2941 gggccttcac tagcatcaac taatagaata actgtgtcaa cagttttcat aacacgttca
     3001 acttcagaag aaaagtcact gtgtcctgga gtatcaacta tgtttatttt gtaatcttca
     3061 tagtttattg cacagttttt tgaatatata gttatgcctc tttctttttc aaggtcgtta
     3121 ctgtccataa cgcagtcttt aactacttcg ttctttctaa aaacaccgct ttgagaaagg
     3181 aaagcatcaa ccaatgttga ttttccagcg tcaacgtggg ctataactgc gatatttata
     3241 attttatgtt tttgtgacat ttttgttgct cccttcgatt aaaataactt attaattata
     3301 gcacaatata ttattaggta caaggaggaa tatattaaat tgtattaata tagtttaaat
     3361 tgaataaaac tgttagtttt gcatctgaaa attattttgt ttaaaaaaat tttttagaca
     3421 aataataacc ttaatagata aaaagattaa taaaagagta aaggatgata tatgttggaa
     3481 gtaaccaaaa taataattaa atattttgca agtgaaagtg atacttttgt aaaagaagaa
     3541 gtttgtaatt ttgaagaact tcttaagtgg tctcataaat ttattgaagg taaaaagata
     3601 gaagatgttt tgtatataaa gtctaaagaa aactataaag taaatgtgat taagtcttta
     3661 gaaattaagg aattggaaat agtgagaaaa caacagatac caccgtacat gtagtattaa
     3721 taatatataa aattaatatg taatactcgc aataggatat aaatggtata tatggtaata
     3781 atttatatta aaaaaacttt tataaagttt atatttgggt ataaaagtat taattagaca
     3841 ttaattagga ggaacttaaa tatgataaga gtatgtccaa actgttcaaa tgtggatgta
     3901 aataagctaa aaactttggt tggagaagaa aatgtaaaga ctggttgtat aggtcagtgt
     3961 agagcattta aaaaagaagc tgttggaata atagacaatg agcttgaaat aaaggaaaca
     4021 gaagcagagt tttttgaagc atgtaaagaa taaattttaa ataaaatata ataaatagag
     4081 catctaaatt gtataagcat taaacaattt agatgctcta tttttatata tacatttaat
     4141 tatgagtata cttttctatt aaatacaaat aaatataact aatacattta tttattaaaa
     4201 aaatattgat ttaatatacc tatagggggt ataataaaaa tatagatttc taaataagta
     4261 atttatcaat attacatatc aaaataaaac gttacaaatt agctgaaaat atattgaaag
     4321 aaaggaagat tttattatga gttcaaataa agtggtagaa agttataaaa ttacaggtat
     4381 gacatgtgca gcttgtgcaa aggcagtaga gagagtaact aagaagatgg atggagtata
     4441 tgaccaaagt gtaaatatag ctactgaaaa acttaaaata gaatatgata attctaaggt
     4501 taattttgat gatataaagc aggttgtaga aaaagcagga tatggaatta taaaggaaga
     4561 aagtaataaa aaaattgata tgaaaattga tggaatgaca tgtgcagctt gtgcaaaagc
     4621 tgtagagaga gtggtaaaaa aattggatgg agtagaaagt attagtgtga atattgcaac
     4681 agataaagct aatatagact atgacccatc aaaagtaaaa ttatctcaaa taaaagcagc
     4741 tattgaaaaa gctggatata aaccaataga agaggtaaaa aataaggttg atgtggatga
     4801 agataaattg agaaaagaaa gagaaatgaa gtctttattt gttaaattta tagttgcaat
     4861 agtatttgca gtacctctgt tttatatagc aatgggacct atgataataa agcctatagg
     4921 gccatggcct ttgccagaaa ttataaatcc tatgacaaat acttttaatt atgcattaat
     4981 tcagttaatt ttagtaatac cagtaatgat agctggttat aaattttata taaatgggtt
     5041 taaatcactt ttttctttaa gccctaatat ggattcttta gttgcaatag gaacattagc
     5101 cgcattttta tatagtctat atacgacact acaaatagcc aatggacaaa ttcaaggtat
     5161 gcaccatcat cagttgtatt atgaaagtgc aggaataata atagcactta tcttacttgg
     5221 taaatattta gaatcaaaat caaaaggaaa aacatcagaa gcaattaaaa agcttatggg
     5281 gctacaacct aaaactgcaa tagtgttagt tgatggtaaa gaagttgaaa ctcctataga
     5341 agaagtagag ataggagata tactattagt aaaaccagga actaaaatac ctgtggatgg
     5401 agttgtaata gaaggatata catcagttga tgaatcaatg cttacaggtg aaagtatacc
     5461 agtagaaaag aatgttggaa gtaaggtgac aggagctagt attaataaaa atggtgttat
     5521 taagtttaag gctgaaaaaa ttggtggaga tactgctctt gcccaaataa taaagttagt
     5581 tgaagatgca caaggtacaa aagcacctat tgctaaactt gccgatactg tatctggata
     5641 ttttgtacca atagtaattg caatagctgt agttgcatca ttattgtggt ttttaatagg
     5701 aggaaaagat atagtatttg tacttactat atttatatca gtacttgtaa ttgcttgtcc
     5761 ttgtgcatta ggattagcaa caccaactgc tataatggtt gggactggaa agggcgcaga
     5821 aaatggaatc ttgataaaag gtggagaggc attagaatct gcacataaag taaatacagt
     5881 gatatttgat aaaacaggta ctataacaga aggaaagcca aaagtaacag atatagtatt
     5941 aaataataat gtaaaagaag aatatcttat caagattgct tcaagtgcag aaaaaggttc
     6001 agaacatcct cttggagaag caatagttaa gtatggagaa gagaagaata taaaatttga
     6061 aaaagttgat aattttaaag ccataccagg tgctggtata caagttacta ttaatgatga
     6121 aagtatatta cttggaaata gaaagcttat gaatgacaat aatataaagc ttggggattt
     6181 agaagaaaaa tcaaatatat tggcaagcca aggtaaaaca cctatgtata tagctgtaga
     6241 tggaaattta tcaggtataa tagcagttgc agatgtagtc aaagaaagta gtaaaaaagc
     6301 aatagaaata cttcatgata tgggaataaa agttgcaatg gtaactggag ataatgcgaa
     6361 aactgcaaat gctatagcaa atcaagtcgg tatagatatg gttttggcag aggttttacc
     6421 agaagataag tcaaaagaag ttgaaaaact acaaaatcaa ggaaagtttg ttgcaatggt
     6481 aggggatgga ataaatgatg ccccagctct tgcaaaagca gatataggga tagcaatagg
     6541 aagtggaacg gatgtagcca ttgaatctgc ggatatagta cttatgaaga gtgatttaat
     6601 tgatgttcca acagcaatta aacttagtca tgaaactata aaaaatataa aacaaaacct
     6661 attttgggca tttggatata atacaattgg tatacctgta gcagcaggaa tattatatgt
     6721 atttggagga cctttattaa acccaatgat tgcagcagca gcaatgagct taagttcagt
     6781 ttcagtcgta tcaaatgcac ttagacttaa aaacttcaag gcatataaga gagattagat
     6841 taataaatta aataaaatgt atccaaaaga gctatcttta aattaaaagg attaatttac
     6901 aaattaagat agctcttttt tcatgtaaaa ttcacaacaa taagataaaa tataagtaat
     6961 atacgaaatt ttaaataaaa tagaaaggat tgatagaatg aattcgtcaa tacttgtaat
     7021 tgaagatgat tcaaatatac aagagttaat atcagaattt ttaagtgcag aaggttatca
     7081 agttgataca gcaaatgatg ggttggaagg tatacaaaag tttaagcaag gaagttatga
     7141 tttagtaata ttagatataa tgatgccaaa cttagatggt tatggagttt gtaaaatgat
     7201 aagaaagtca tcaagcgttc caattatatt tttaactgct ttaaatgatg aaggagacca
     7261 acttaaaggg tttgacttag aatgtgatga ttatataaca aaaccatttt catttaatct
     7321 tcttataaaa agagtagaag caattttaag gcgaagtaac aagactataa atgacaaatt
     7381 tatagttttt gaaaaattaa aattagattt aaacacatat attgcagaga tagatggaga
     7441 acctatagaa cttacactaa aagagtttaa tatattaaaa gctttgatag aaaagtatcc
     7501 acaagttata actagggaag gtcttttgga tagtatatgg ggttatgact attatggaga
     7561 tactaggatt gttgatgcac atataaaaaa tataagaaaa aaaatatcat taccatatat
     7621 taaaactgta aaaggaatag gatatacact tgagaaggat atttgataag tgggaaaagt
     7681 taagtataaa gtataagtta ttttcaataa cgacatcatt attaatagca ttagctttaa
     7741 taatatatct aattttatat tttttattgc catcatatta tcatgagtat aaaattgagt
     7801 ctcttcaaga aagtttgaaa tctttagtag atagttcaat tcatttcgat acatatactt
     7861 tagaagaaag actttattat atggctaaag accaaaattt ggctatttta ttaaaagata
     7921 accaaggtaa aattgtatat ggtaagaatg aagtagttat tttaagatat agtaagtaca
     7981 tgattaatag tcttgaggat gagtatagaa cttctatacc tatatatact aaagatgcaa
     8041 aagatggtcc atatacttta gaactcgtta tgccattaca accaattgac gaagcaaacg
     8101 aagttataag aaaactaatg ccatacatca tatcaatagc aatattgata gcgattattg
     8161 gagcatatat atattcaatt gtaataacaa agcctcttat aaacataata gagagtgaga
     8221 gagaacagga atatagaagg aaggattttg ttgctacaat atcacatgaa ctaaaaactc
     8281 caataaccat aataagtggt caaattgaag gtatgattta tagtgtaggt aagtataagg
     8341 atagggatac ttatctgaaa aaatcgtatg agtgtacaca agaattaaaa gatttagtaa
     8401 atgaaatgat agaggtttca aaatcagaaa tacttgaaaa agatttgaaa ttggtatcaa
     8461 taaatataag tgaattatta aatagacttg taaagagaca agtattttta atagaagaaa
     8521 aacatatgaa aactatttta aaaatagaag aaaatttgga aattaaagca gaccaagaga
     8581 gaataactaa ggcaatcaat aatataataa ataatgccat aaaatattct cctgaagaat
     8641 cagagcttat aattagactc tatgataaaa ataaacgaat aagtaaaaag acttctaatc
     8701 aaagagtggt tttagaaatt gaaaatactg gtgtaactat cgagaaacgt tatttagaag
     8761 aaatatttaa tccattttat agaatagaaa aatctcgttc gagaaaaact ggaggaagtg
     8821 gacttggact ttatatagtc
//
LOCUS       DAGSHT010000140         8740 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGSHT010000140 DAGSHT010000000
VERSION     DAGSHT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.145"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            39..1127
                     /locus_tag="MJB72_002744"
     CDS             39..1127
                     /locus_tag="MJB72_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBY8263820.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1155..1946
                     /locus_tag="MJB72_002745"
     CDS             1155..1946
                     /locus_tag="MJB72_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBY8263821.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2061..2615)
                     /locus_tag="MJB72_002746"
     CDS             complement(2061..2615)
                     /locus_tag="MJB72_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY8263822.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            2811..4013
                     /locus_tag="MJB72_002747"
     CDS             2811..4013
                     /locus_tag="MJB72_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBY8263823.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4070..5050
                     /locus_tag="MJB72_002748"
     CDS             4070..5050
                     /locus_tag="MJB72_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBY8263824.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5293..6246
                     /locus_tag="MJB72_002749"
     CDS             5293..6246
                     /locus_tag="MJB72_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBY8263825.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            6236..7192
                     /locus_tag="MJB72_002750"
     CDS             6236..7192
                     /locus_tag="MJB72_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBY8263826.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            7189..7944
                     /locus_tag="MJB72_002751"
     CDS             7189..7944
                     /locus_tag="MJB72_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8263827.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            8036..>8740
                     /locus_tag="MJB72_002752"
     CDS             8036..>8740
                     /locus_tag="MJB72_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8263828.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSH"
BASE COUNT         3420 a          885 c         1480 g         2955 t
ORIGIN      
        1 taaaatatat cacgatttta agataggagt tgagtcatat gagagatttt agcacgtatt
       61 ctaaagatga tgttattttt ttattaaaag atatatcaaa tttaattgaa gaagaaggaa
      121 ataaagaaag agaattaaaa atacaaaatg gtagacatta ttcagaaatg ataccaatag
      181 agtatgaagt tagtgaagat tatctgaatt tatatcatga gaaactaaga gaaaataaag
      241 ataaacttgc attttctata ggagttatgt gtgaaaaaat aattaaaaaa aatggaaatg
      301 atgtagtact agtttcactt gctagagcag gaacacctat tggtattttg gcaaaaagat
      361 atataagaag caagtataat ataagtttac ctcactatac aatctctata ataagagata
      421 agggaataga tatgaatgca attgaatata tagtaaagaa tcatccgagc tcaaaaattc
      481 aatttttaga tggatggact ggtaagggaa ctatatcaaa agaacttgaa aaagcatgtg
      541 atgaacttaa tgttaaattt aaaaaatatt ttgactcaac acttgctgta ttagctgacc
      601 ctgctggata ttcaggactt tatggaatta gagaagattt cttaatacca agtgcatgtt
      661 taaattcaac agtttctggt ttagtaagta gaactgtact aagagaggat ttaattggta
      721 aaaatgactt ccatggagct aagttttata gacatttaaa agataaagat gaatctatga
      781 actatatcga aactatagaa gaatgtttta aaaatcaatt taagaatata tctgatgaag
      841 ttgaaaactg ggaaagtgac attataacaa gagatggata ctttgatgtt ttaaatataa
      901 aagaaaagta taacataaca gatattaact ttatcaaacc aggtgtaggg gaaacgacaa
      961 gggttttact tagaagagtt ccgtataaga ttcttgttaa agacttaaat gataaatctt
     1021 tagaccatat attcatatta gctaaggaga aaaatgttga agttgaacaa atggatttaa
     1081 aagcctataa atgttgtgga ataataaaaa atatgaaaga tatttaggaa atatattaga
     1141 caggagaata ttgaatgatt gttttttcag atttagacag gagtatcatt tactccaata
     1201 aatttttaaa tgctgatagt aaatatacta atatagaaat ttatagggaa aaagaaatat
     1261 cttatatatc tttagatact ataaatttaa taaagcaaat acaatattat ggtatgttta
     1321 tacctacaac tactagaaca gtggagcaat ttaagcgtat agaatttaat aaatatggga
     1381 tttattttcc ttggagtata acttcaaatg gaggagttat acttaaagat aatgaaatat
     1441 tgaaatcatg gagtgaaaaa atagataaac taaaatgtga ttatgagccc attgaaagta
     1501 tgattcataa atttaaagac tatttgaatg tagatggaat aactaatttt aaagttgcag
     1561 aagatacatt tttttatata gttgttgatt tatcaagatt taatttggat tcaatcaaag
     1621 aatatacaaa tatattggaa tcaaaaaatt ggaagtttta tgtgagtggc agaaaaatat
     1681 attttatacc aaaagaaatt tcaaaagaaa atgctataaa atacttgact aaagaattag
     1741 gaatagaata tttttatgca gttggagatt ctattatgga ctatggtatg cttaatatct
     1801 caaataaatc ctatgtattg aagcatggtg atataaataa aaatgaaata gaaaattcat
     1861 ttatatcaag ttctttcaat ggaatgagtg gaactgaaga gatactatct aacattttag
     1921 acgagaactg cctacttaat atataatgac tgatatataa ataataaact agaaaaatct
     1981 gaaataaatt atagtaaagt ttatcatact aatttatttc agatttttat caatttatat
     2041 ttaagtaaga actcaatatg ttaaaatgaa ggtggagtca ttgcccagac tgagatacat
     2101 acctcatcat cataattttc atacacatgt ggtatagtac tgttaaaata aaaactatct
     2161 ccttcctcca atagatattc cttatttcca agtgttatct tcattttacc ttttattata
     2221 tagccacatt cttctccttc gtgtgtcaca aattcactgt ttttttgtgt atgaccatct
     2281 agtgtaacaa gtataaattc tatttttttc ccagataaat taggaacaag aagttcataa
     2341 aattttgtag aatcattagt tactattttt tttcgattat ttttctttac tactatatca
     2401 gagtcttctc ttccaatctc ttcaaaaaaa taacctatat ttatatcaag agcttttgat
     2461 attttccaca ttgctacaat agatggaaca accatattac gctcaatttg acttattaag
     2521 cctgtactta aatctgaatt ttttgctaaa gtagagatac ttatattttt agattttcta
     2581 agagatctaa ttttgcttcc aacatctata ttcattagat acaaaccccc tcagacatga
     2641 ttataacata taatttataa agattaataa aataaaaaaa aagttttaca atacaagtat
     2701 tttttatgct atattgaatt ttctgaaaag atgcattata atagtattaa tattcaatat
     2761 attgaatatt gataacgata ttttaattta atgaaatgga ggtaattaag atgaagagtt
     2821 ttgtaagtcc tgataatatt tgggtattat gggcagttgt aactggatgg gcagcattta
     2881 gtatttattt ggaacaaaag tacaattggg catcaaaagt gtcgggagca ataatagctc
     2941 ttgtaggagc aatgttatta tcaaatctaa atattatacc agtagagtct gttgtatatg
     3001 accaagtatg gggatatgtt gttcctttag ccatagcttt attgttatat caatgtaaca
     3061 ttaaaaagat atggaaagaa agtggaagat tgctcattat atttcttgta ggttctgtag
     3121 gaactgtact tggagctatg attggatttt tagcattaaa aaatgtagta ccagatttaa
     3181 atattgttgc agctatgatg acaggttctt atataggtgg aaatgtaaac tttgctgcta
     3241 tgtcaggggc gtttgatgcg ccaggagaat tagtatcagc aacagttgta gcagataatc
     3301 ttttaatggc attatatttc tttgtcttaa tagctatacc atcaattgga ttttttagaa
     3361 aacattttaa acatccacat gtagatgaaa tggaaagtat aggtataact gaaggagaaa
     3421 cagtagcagc aaattattgg ggaagaaaag aaatatcttt aaaagatata gcaatagcca
     3481 ttggtagcgc ctttataatt gttgcagtat ctgtagaact atcaacttgg tttaaagcta
     3541 taataccatt atcaaatcct tttttagcaa tgttaaatac tttatttgga aatcaatatc
     3601 taataataac tactttgact atgttgtttg ccacatttat gcctaatttc tttggtgata
     3661 taaaaggagc acaggaattg ggtacatttt taatatatat attctttgtt gtagtaggtg
     3721 ttcctgcttc aataacatta ataattcaaa aatcaccatt actactttta tactgcggaa
     3781 taatggtact aataaacatg ctagttacat ttatagttgc aaaaatattt aagtttagtt
     3841 tagaagaagc tattcttgca tctaatgcaa atattggtgg tcctacaaca gcagcagcta
     3901 tggcaatatc aaaaggatgg tctaagctag tagggccaat acttatagtt ggaacatttg
     3961 gatacatagt cggtaattat tttggtcttt tagtaggaaa tattttgata taattttacg
     4021 tgaaaaaaag ttgtttatta gaaagatttt tatttaattg gaggttgtta tgattttcga
     4081 tggacattca gatacttggg cagatgtaac aataagaagg cttaacgggg aaaaagatgt
     4141 atttagaaga atccatcttg ataattttaa aagagcaaat atagaaggag ctatttttgt
     4201 aatatggata gacccaccat atgataaaga gcctgaaaaa agagttaaac aaattataag
     4261 ttctataaaa gatgaaattg aagatatgaa agatgttgta aacatagtaa aaaaatattc
     4321 agatatagat aaaggtataa gtgaaaacaa aataaatatt ttaattggtc ttgaaggatt
     4381 aagccatatt ggaagaaata tagatttact agattatttt tataaagaag ttggtgcaag
     4441 acatgcatca ttaacttgga atgaacagaa tgaacttgct acaggatgtt taggggatga
     4501 aaatcgtggt ttaagtgaaa taggcaaaaa agcagtcaat aaaatggaaa aattgggcat
     4561 gatagtagat gtgtcacatt taaatgaaaa atcattttgg gatttaatgt ctgttgcaag
     4621 taagccagtt atagcttctc attctaatgc taggagttta tgtgatgcta aaagaaatct
     4681 tacagataat caattaaaag aaatttcaaa tacaggtgga ttagttggaa taaatagttt
     4741 tagaggattt actaatagta atcctaagaa gcaagattta atacatttgg ttgaccatat
     4801 agactatatg gtaaatctta taggtataga acatgtatct tttggttttg attttagtga
     4861 ttacttaggg aaagagacta taggttcttt tactggaaat gctagtgtgc caagtgtaga
     4921 tggtcttgaa agcacacata cagcacagaa catagtagta gagcttgaaa atagaggtta
     4981 taaaaagaaa gatatagagt taatctctag tggtaatttt tataggctct ttaaagatat
     5041 actaaaataa tataataaag aacttaaatt tataaaatag tatatttata caaataatat
     5101 ctacatattt aaattggcgt tcataattta ttaaatttta atgttactag aagtttcaat
     5161 tctagtaaca ttttttatgt taaaaacaga tatagatatg tattgacaat gataattatt
     5221 attgataaac tataaaagtg aaaatgataa taattttcat tttaattaat aaaagatttt
     5281 taaggaggga gaatgaagaa aaggtattta gtcataggaa taataatttt gtcttatttt
     5341 tctttattta taggagctga agatataaat ataatgcata tatttgccaa aaatcagcat
     5401 aaacttatga tatttatcat gagtagaata ccaagattaa taagtatatt gattgctggt
     5461 gtaggaatga gcatagcagg tcttataatg caacagataa gtaagaataa atttgtatca
     5521 cctacaacag gtgctacaat agatgcagca caatttggga tagtaatatg tatgctttta
     5581 gtaccaactg cctctatttt tacaaagaca ataatagcat ttgtattttc tcttgtaggg
     5641 acttttacgt ttatgaaaat aatagggaag ttacaattta aaaatataat ctttgtacca
     5701 ttagtaggta taatgtttgg aaatataata gggtctatga cggattttat cgcgtataaa
     5761 tacgatttga gtcaaaatgt tagttcttgg atgcaaggtg acttttctat gatacttaaa
     5821 ggaaattatg agatattata tatcacaata ccccttataa tattagcata tatttatgca
     5881 aataaattta ctgttgttgg tatgggtatg gattttgcga caaacttagg tcttagttac
     5941 aaaagaattg tcaatatagg tctgataata gtagcacttg ttacagtatg tgtagtcgtt
     6001 acagctggta atatcccttt tataggtcta atagttccaa atatcgtatc cttgtacatg
     6061 ggtgataata taagagcaag catatggtat actggtttac ttggagcgat atttgtactt
     6121 atatgtgata tttttggaag aattattatt tatccatatg aaatatctat agggcttaca
     6181 gttggagtta taggaagtat actattcctt tatttaatat taaggaggaa tgtgaatgaa
     6241 gcttagtctc aatcaaaaaa tttactttgt aagtatatta ctattcatat caatggcact
     6301 ttttataatt tatggaatag acatgaatca tttagaatat gcattatcac aaagaatacc
     6361 aaaaatattt gctatggtat taggtggagg atgtatagca tttactactg tagtgtttca
     6421 aacaattaca aacaatcaaa tattaacacc aagtgtacta ggtttagatt cattgtatgt
     6481 aatgattcaa actataatag tattcatatt tggttcaagc agtagtctga ttataaatga
     6541 aaattataat tttattatta atgtagctat gatgattgga gcttcattgt tattatataa
     6601 gacgttattt gaaaaaaata aaaataatat tttcttttta atcctagtag gaatgatatt
     6661 tggaacctta tttaaaagtg caacaacatt tattcaggta atgatagacc ctaatgaatt
     6721 tttagcttta caaactagta taatggcaag tttaaacaat ataaacacta atgtattgtt
     6781 aatagcattt ataataatta ttgccataat accttttata tatgatgaaa taaagtattt
     6841 agatgtattg tcgttaggta aagaacaagc aattaattta ggtgtagatt ttgataaagc
     6901 agttaaaaaa atgataatat taatagcaat tttagtctca atatcaacag cacttattgg
     6961 acctatgaca tttttaggac ttttacttgc taatataaca agagaaattt ttaaaacata
     7021 taagcataca tacttgattt cgggttctat gttgataggg atgattacat tgataatagg
     7081 tcaatttttt atacaacatg tatttaagtt tgatacaact ttaagtgtag taataaattt
     7141 tataggagga atttatttta ttcaattact attaaagggg gcaaatagat gatagagata
     7201 aaaaatatat ttaaaagata taagaataaa aatgttgtag atgatgtctc ttttagtata
     7261 gaaaagggaa agataacatc ttttatagga ccaaatggag ctggaaaaag tacagtacta
     7321 tcaatagtta caagacttat aggaggtgat ggtggagaag taataataga aggaaaaagc
     7381 ttgactaatt atagcaataa agaattagca aaaaaaatag ctattcttaa acaatctaac
     7441 aatataactt taaagcttac aataagagaa ttagttggat ttggaagatt tccatatagt
     7501 gaaggtaatc taactaaaga ggatgaaaat tatattgatg aagcaataga atatatgaag
     7561 ttgactgata tacaacataa gtatttagat gaattgagtg gaggtcaaag acaaagagct
     7621 tatatagcaa tggtaatagc tcaagataca gagtacatat tacttgatga accactgaac
     7681 aatttggata tgaaccattc cgtacagatg atgaaggtac ttagaggtct ttgtgatgaa
     7741 ttagataaga ctatagtatt agtaatgcat gatataaatt ttgcatcatg ttattcagat
     7801 aatatagttg cattaaaaaa tggaaaagtt gaaaaagtag gtcgtacaga tgagatagta
     7861 aatgaaaaag ttttagaaga tatttatgag atgaatttta acattaagaa tataaatgga
     7921 aatcgaatct gtatttattt ttaaaatatt gtatatgaaa ataaacttgc tatatgattt
     7981 tttataaagt ttttaaatta aaataaagat taataggaga gaggaaaata ttgatatgaa
     8041 taaaaaagca gcaatagtag cagcagtagc tattatagga ttagtgacag tgtttgcatt
     8101 aggtggaagt aaaaagaatg aatcaaaaac atcagaaaat tcaaataata ctatgaagat
     8161 aactcataac ttgggagaaa cagatgtaaa attaaatcct aaaaaagtag tggtatttga
     8221 ttattcggct ttagatacaa tggatgcatt aggtgtagct gagactttag taggacttcc
     8281 aaaagcgagt ttaccagcat ccttagaaaa atataaggat gacaagtatg cagatttagg
     8341 aggattaaaa gaaccagatt tagaaggtat aaagtcggca aatccagatt taattataat
     8401 aaatggaaga caagaagatt tttatgagca attgtcaaaa atagcaccaa caataagtac
     8461 aagtaaagat gataagaagt acttagaatc tgttaaaaat aatatagaca aaatagcaaa
     8521 aatatttgga gtagaagaaa aagcaaatca agaatttagt aaaattgaaa agaaaataga
     8581 aactttgaat aaaaaagtaa cagataaaaa tttaaatgct ttaactataa tggtaaatga
     8641 aggtaattta agtgtatttg gtgaagaatc aagatttagt atattataca atagttttgg
     8701 atttgaaaat aaagataaaa atataaaaga atcaagtcat
//
LOCUS       DAGSHT010000141         8714 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGSHT010000141 DAGSHT010000000
VERSION     DAGSHT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.146"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..633
                     /locus_tag="MJB72_002753"
     CDS             <1..633
                     /locus_tag="MJB72_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263829.1"
                     /translation="LKEVTKNNEEIVPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGE
                     DSVKKRKDSILNFEFGLVAFVNKNYSNLTEKEKLIRQINIVNYENSINDIMDPSGSFF
                     ELANQNYQVNEFLLKNGKDYKEWSYEGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIK
                     TDLSEEQKKSLNDKLKKISSSDTLSEEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(790..996)
                     /locus_tag="MJB72_002754"
     CDS             complement(790..996)
                     /locus_tag="MJB72_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263830.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(1399..1794)
                     /locus_tag="MJB72_002755"
     CDS             complement(1399..1794)
                     /locus_tag="MJB72_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY8263831.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            1935..2327
                     /locus_tag="MJB72_002756"
     CDS             1935..2327
                     /locus_tag="MJB72_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263832.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(2427..2843)
                     /locus_tag="MJB72_002757"
     CDS             complement(2427..2843)
                     /locus_tag="MJB72_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY8263833.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(2858..3769)
                     /locus_tag="MJB72_002758"
     CDS             complement(2858..3769)
                     /locus_tag="MJB72_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8263834.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(3951..4973)
                     /gene="splB"
                     /locus_tag="MJB72_002759"
     CDS             complement(3951..4973)
                     /gene="splB"
                     /locus_tag="MJB72_002759"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBY8263835.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            complement(5200..6330)
                     /locus_tag="MJB72_002760"
     CDS             complement(5200..6330)
                     /locus_tag="MJB72_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY8263836.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(6718..8655)
                     /gene="htpG"
                     /locus_tag="MJB72_002761"
     CDS             complement(6718..8655)
                     /gene="htpG"
                     /locus_tag="MJB72_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBY8263837.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         2943 a         1431 c          928 g         3412 t
ORIGIN      
        1 ttgaaagaag ttactaagaa taatgaagaa atagtaccta aaaacttaga aggtttaagt
       61 atagaacaat taatagagat tagagataaa gccattgata aaaatttaga aggtcttagg
      121 gaatttggag aagattcagt aaaaaaacgt aaagattcaa ttttaaactt tgaatttgga
      181 ttagtagctt ttgttaacaa gaattatagc aacttaacag aaaaagagaa attaataaga
      241 caaattaata ttgttaacta tgaaaactct ataaatgata taatggatcc ttcaggttca
      301 ttctttgaat tagcaaatca gaattaccaa gtgaatgagt ttctgttaaa aaacggaaaa
      361 gattataaag aatggtcata tgaaggtcta gattttctaa ttgatcatct agaagaactg
      421 tgcaaatttg ttgattttga tgtaaaagat gtaatagata ttattgaccc tacaataaaa
      481 acagatcttt cagaggaaca aaagaaatct ttgaatgata aattaaagaa aatatcttca
      541 agtgatactt taagtgaaga agttaaaaag gagataaaaa actgggaaaa caatttaaat
      601 aatttaaata tgaataaaaa taattctaat taattttctc ataagaaaaa tgcttatatc
      661 gtgataatgt gaaatgtaca taattataaa caaaaaaggg gctgtgacac taaaaaatag
      721 tgctacgccc ttttttattt gtaataatca tcaatacaaa ttattttatt atatgtaatt
      781 atttatgtct taaccaattt tatcgaaaat aacagtcttc tcttttaaag agatttttcc
      841 tacttgatat ccatattcat ttaattcctt tttatatttc aaaaacgaat ggtctatctc
      901 tatcccagca atatctttta tttcttcaaa agttaattta aacgattgac tattgttttt
      961 cttaacatat tcccataaag aactatattt actcatctta tatcactcct tttaaaatca
     1021 tatcttatct attaatttat attctatcat tattaatgta taaagtcatg ctacttatta
     1081 tttatatata aaaatatcca aataataagt agcaattttt tatagtcata atattttttt
     1141 cagattaaat aaaaatcaat tatagttaaa atatttttta aaagaaatct atcccattat
     1201 aaagtaaatt ttaattatct tatttctagt atgtaattat tcaaaatagt atatcattat
     1261 tgaaatacac atctaataac taaaaatttt tacatattca ttatttaaaa atctttttat
     1321 tgcatttaaa ttaaatctat caatctttca gtaataacgt tttcttaatt ttcactatgg
     1381 atgtcgattc aattataatt aaagctctac atcaatcttt ttaccactca ttgaacaaca
     1441 actaactttt gccttatcaa tagagaatat ttcaaactta ccatctccca cagagtacat
     1501 ctgacctaaa cttggcatta tttcaagtgc cttagcttgt gtttcttttg atgtaacaaa
     1561 tactgcttca cctaaaatac gtagagtatt aaggttttgg tctatacaac aaagttcaac
     1621 ctttggatta aaaataagtt gcttaaacat atccttttga ttagatgtgc aaaatgtaag
     1681 tttaccattg tattccataa caaatcctaa aggacgcaca tgtgcttgat tgttctcatc
     1741 aactgttgct aaataataaa ctcctgcctt gttcaaaaat tctaatgctt tcataattct
     1801 tacacccctt ttaaatactt gtattaagga ttatcctttg atataatttt aatataaaat
     1861 acagaaaaaa caagtatgca gttttttctg ataaagtata aaaaagatac tactataact
     1921 aaggagtaat taaaatgagt ataaatactg aaaaaaatat aactaaaaac gttgaagaat
     1981 taacctgtcc aatccgttat gcattagaca ttgtcggtgg taagtggaaa ttacctatta
     2041 tatgtatgtt agcagttgaa aatccaatta gatatagtag cataaaaaga aaattggatg
     2101 gcattacaaa tactatgcta gctcaatcat taaaagattt agagtctact ggtattgttc
     2161 atcgtaaaca atataatgaa attcctccaa aagttgaata tacattaaca agtaaaggta
     2221 aaagtattgt gcctatttta caacaatttg ctaattgggg tgctactaat atgcaagaaa
     2281 aaaacacatg tggacttagt tgcaaagaat gtagaaagat aaagtaaatt taaatttact
     2341 aaattattca tattatagac tatatattaa tttatctgat tgaatataaa aacaggatga
     2401 cttatatatt catcctgttc tatatgttaa gctctaaatt taattagttc aagcatatcg
     2461 tttaatacca ataaactatc tacttgaatc attagccatt tacttccatt aaaaaagttt
     2521 acctcattat atattttttt gatataatca caacaagtat taataaataa atcaacctct
     2581 actctattgc tttcacttac aataaccaaa gctttaaaat aaccttcttg tggatataaa
     2641 gtacatagtg atttacctct ttttttatat tttacattcc aacctctttg cattgaacat
     2701 ttactgtatt caagcttagg ttctacatta taagtagaaa ccaaagtttt atttaacttg
     2761 tcccataatg gattattaat aaattcatta atttgctcaa atgtaggttt agtatcttga
     2821 aaaaacgcat cttgccaatt catattcatc atcctttcta ttgttcaata ggaaccggaa
     2881 caatccaatg ttccagatat gctgcttcag gtgtagtggg gtaatacatc tcaattgcaa
     2941 aatcagtagt tgttaaatta tgtttcttca tccatctttc cataaattta tctgctttaa
     3001 aaacagctga attaactaac tcattaaagt tttctgcctc aaatccacat actaaatatt
     3061 ctttagctgg caactcaaaa gtactatatc cttctactga tgtacctgac tctacctcta
     3121 cacctgccat atacatacaa tttccttctt ttgcatttcc catataaaga gcagcaaatt
     3181 catttccacc tggaatttga tttggtatat tatgtctttg acttccaagt ttcatccata
     3241 attcctctac tattgcaatt ccagttgttt caccgcccac caactggcaa ataggtactt
     3301 ctatttctat tccaacaaat gtccttggat tactcaatgt cttacgattg acttcaatca
     3361 ctatatcatc tgcaatcagt ggaacacctt catcaaccat agcataattt aaaattaatt
     3421 ctggcttaat aaaatgattg agttttactg gatttgctct atatttttct ggagttaatc
     3481 catatgcttt tttaaaggaa cgtgtaaatg tctcatgact agcaaatcca aaatttaaag
     3541 ctacatcaag tatacgattc ttatgttctg ctaagtattc agaagctctt gcaagacgac
     3601 gaagctttat atactccatt acagtcttat ttaccaatct tttaaatagt ctttgatagt
     3661 aaaatggtga taaatttgct ttatttgcta attcattaat tgatatttct tctgataaat
     3721 ttgcttctat taaatccaat gtaatttgaa tagactccca tgcatgcata ctataactcc
     3781 tcctgatatt tttgttactc ttatccttta tagtatcaaa actagaaaaa atgttcttga
     3841 tagatagtgc cctttttaat tttcttatat gataatgcta aatctcattg ctttattata
     3901 aacataaaat atataaaaat aaaatatagc aaatctttag gtaagatttg ctatattata
     3961 tattttatta aactattttt aaatactcca tttatttcct taagaaagaa ctctttcata
     4021 tcatctaatt gttctttatc atacacgtac ttaccatatc caaattgacc gtatttaaac
     4081 tttctatctt cattttccat tggcaatagt gtatctggat aaatctctag tattctattt
     4141 tttgctttag ttgtatatct atgagatata acttcaaata taattttttt atcaaaatcc
     4201 tttggtaaag cttcttttat acttataatc agatttttat actcttcttt ccaaccttca
     4261 tataaaaaca caggtgctat tatgaaacca atctgataac cagccctagc aagttttaca
     4321 gctgactcta ttcttttatt tgcagaggct gtataatgct catgttcatt tataattcta
     4381 ggagtattga tactaaatcg tatttctgta tgattattat gttcagcatc caataagcta
     4441 tccacatcac tgtatttagt aacaaatcta aatcttcctt tttcctctct tccaaaaaac
     4501 tctattgttt tcttcaaaga attagtatat ggctctactg gaactgggtc tgatgtagct
     4561 gccccttcaa atatggttac ctgtggcttt ctatcttcaa tatatttttt tgtctgttca
     4621 agtatttcat ctacatttac aaacaccctt ataaatggtt tatcacctaa ttgtgtgttc
     4681 aaataacaat actcacatct tcccatacat ccactgacaa gtggtagttg ataattagca
     4741 gaaggtttac atgtttgaaa cttcagactt tttctaatcc caactaccat agtctgcttc
     4801 ccacttctat attgttcata tagattttca cctggaatat gttctttaat cctatttgat
     4861 gatatattaa ttatctctac atctttatta tctctaaatt tattgtatat ttttttaccc
     4921 acatcagtgt ctaaagttcc tttttcgaat attattcttt tgggattaaa catttatttc
     4981 acctctactt aaagttttta aatcgaagaa ttttataaaa tattacttaa ttaatcttcc
     5041 cttttttaaa ctcctttatt tattagaaag taaaataaaa gcttaagaca aatttattat
     5101 atatataact tctatacaca atttttttac tattaattac ttataatttt ttattattaa
     5161 tttataattt ttatataaaa ctaaatatgt aaagtcaaat tataaagact tatggtacat
     5221 ttcctttatt tgttccttat taaaatactt tggatgtctc ttaatatttg caactgatat
     5281 tttcatacaa ttatcagtta accaatctat atctgatttt tcaataccta aatcacttag
     5341 ctttatatct aaatctattt ttttaaggaa atttctaatt gcatctgaac aatcttctgc
     5401 acaagttcct cctaatacct ttgaaatatt accaaatctt tcaatatcac tatcccatga
     5461 actttctata atagtaggag tcaaagctgc aagtcctttt ccatgcatta catctctaag
     5521 accacttaca ggatgctcca ttccatgtgg caaggctgta cccgctgtac caatcgtcat
     5581 tcctccaata gtacttgcaa gtgttatttt ttcccatgct tctaaatcat taacatcatt
     5641 atataccttt attaggtttt ctgatataag ttcaatccca taaatagctt gtacatctgc
     5701 aagagggttt cttccattag aaagatatgc ttccatatta tgggcaagtg catcaaatcc
     5761 aactgatgca attatatgtt ttggcattgt aaccataagt tctgggtcaa ttattgatgc
     5821 ttttgcatac atactattct tctttataga ttttttatct ttagtttctg aatttgtaag
     5881 tactgcaaaa caatttcctt cactacctgt acctgatgtt gttggaacta agattatagg
     5941 taaagcttct tcaccttgtc taattccaaa tatataatca tttaaatctc ctgggttttt
     6001 ataagaaaaa gctatactct tggcagcatc cataatgctt ccaccaccaa gacctaaaac
     6061 acaatcacat ccagttgatt ttataatttc tactcctgaa tatatagtag ttataagagg
     6121 attttgttct actctatcaa aaacttcata ttctatttta gagtctttta gtaaatctat
     6181 tgttttatca agtaatccag ttttctttgt actattttta cctgttacta tcaatacttt
     6241 tttgccatat ttagatactt cttttcccac atcattaatt ctgccgctcc caaataagat
     6301 atttactggc aaattataat taaaattcat gctaaaacct cctatttcta ctttagtaat
     6361 aattttatgt ttttaacaaa gtgtaattat gtatttaaac ataaactaaa aaacaatctt
     6421 ttacttaatt atatgtatta tcttattata tatcaagtta actatattta actaaataga
     6481 gttttttatt agaacctact taaatattta aaaattaacc aaagataaaa aaataaagaa
     6541 cggtctgtct caaaatagag ataaatatat ttttcattgt tgaaatgatt aaaataaatt
     6601 taatttcaca taaaaaaagc gcctcatgaa ctgaaaagtt cattttgaga cacttctttc
     6661 ttatcactga tattgaatac tttttataaa tttgcttcaa tgtattcaaa ttcaatctta
     6721 taattctatc aaaagactca taagcttatt acttctttgt acaaatgaat ctaactcatc
     6781 tggctttaat tctttattag aaagtaaagc taaatctgct atctggttac aaataagcgt
     6841 aatcttatct ttcttttcct catcatcttt aagagaaacc aattttttga ttattggact
     6901 gttctcattt attacaagag ttttttcttc ttcaaaattc attcctaaat ccattccagc
     6961 aaatcttgac tgcatttctg ccattctaat agattgttct gatactaaca ccatagctgg
     7021 agtgtcttca tttttcaatc cttctacaga ataattcttc actctttctc caacagcttc
     7081 tttaaacaga ccttctatct tagctatctc ttctttgttt tcttcactat ctttattttc
     7141 atttttatct tttaatacat cagaaaggtc tgcatctatt ctattaaact taactccatt
     7201 ctctttatat tccataaatg aaataaaatg agtatctatt gatgagttta aaacaacagc
     7261 acttaagtcg tattctttaa atagtttaat gtattgagat tgctgttctt tatcacttac
     7321 ataaaatacc ttattttcat gtttgtcctt acacttttct aaataatctt gtaatgttat
     7381 atactcatca tcaatagttt tgaataatat acattctttt accttctcat agaaactttc
     7441 atcttttaag caaccatatt taataaatat ttgtatatca tcccaaaact tattgtattc
     7501 ttctctttca tttttacaaa gagattttaa cttatcagct acttttttaa ttatatgttt
     7561 agaaattttg cttacatccc tgtcattttg taagaaactt cttgatacat ttaatggtaa
     7621 gtctgggcag tctataactc cttttaataa aagtaaaaat tctggtataa cttcttttat
     7681 attgtcagcc acaaacactt gattgttgta taattttact tttccttcag tcagttcaaa
     7741 ctcatttttt aatttaggga agtaaagtat accctttaaa ttgaaaggat aatctacatt
     7801 taaatgtatc caaaataaag gttcatcaaa tacattgaat acctttctat aaaactcttt
     7861 atactcctca tcagtacagt cttttggaga ttttaaccat agaggattag tgtcgtttaa
     7921 aggctcaatt acttttgcat ctacaatctc ttcttttcca tcttcctttt gttttttagc
     7981 tttttcttct gcttctttag cttctctttc taatctttca acatcttcta aatatatttc
     8041 tactggtaag aaagaacagt atttatttat tattcctctt acagtaaatt catctaaaaa
     8101 ttctttacta tcatcatcaa taaatagtgt tatagtagtt cctctatcat ttctagcatc
     8161 tgattcagat atttcgtatt ctgtaccacc ttcagatatc catcttacag gagtcgctcc
     8221 ttctgtatat gaaagtgtat ctatttgtac ctttttagac accataaatg cagaataaaa
     8281 tcctaatcca aaatgaccta ttatatcatt tgactcttcc attttatcct tatatttatt
     8341 aaagaaatct tcagcacctg aaaaagcaac ttgatttatg tattttttta tctcttcttc
     8401 agtcatacca attccattgt caataaattt aagtgtacct tctcctttat ttacagatac
     8461 tgtaatttta taatctgatg acttattttc tgatatttct cctagtgata caagtctttt
     8521 atgtttactt actgcatcac atccattact tataagctct cttataaata tgtctttatc
     8581 agagtataac cattttttaa ttataggaaa aatattttct gtatgaattg aaatactccc
     8641 tttttcaaat tccatttttt atccctcctc attttataat taatatttta aattttttaa
     8701 aatggtatgt caat
//
LOCUS       DAGSHT010000142         8612 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGSHT010000142 DAGSHT010000000
VERSION     DAGSHT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.147"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..346)
                     /locus_tag="MJB72_002762"
     CDS             complement(<1..346)
                     /locus_tag="MJB72_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBY8263838.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDL"
     gene            580..1032
                     /locus_tag="MJB72_002763"
     CDS             580..1032
                     /locus_tag="MJB72_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263839.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            1035..1343
                     /locus_tag="MJB72_002764"
     CDS             1035..1343
                     /locus_tag="MJB72_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY8263840.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            1457..2563
                     /locus_tag="MJB72_002765"
     CDS             1457..2563
                     /locus_tag="MJB72_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY8263841.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2638..5316
                     /gene="mngB"
                     /locus_tag="MJB72_002766"
     CDS             2638..5316
                     /gene="mngB"
                     /locus_tag="MJB72_002766"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBY8263842.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5633..7966)
                     /locus_tag="MJB72_002767"
     CDS             complement(5633..7966)
                     /locus_tag="MJB72_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8263843.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(7941..>8612)
                     /locus_tag="MJB72_002768"
     CDS             complement(7941..>8612)
                     /locus_tag="MJB72_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263844.1"
                     /translation="ADAIEIYLLNQGYNVFKGYNGLEGLKVIENQEIHLAIVDIMMPK
                     MDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVTKPFKPLELLARVNSQ
                     LRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDGKYIKTTPIEFKILSL
                     LMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEIDPKNPKYLKVVWGVGY
                     KIEKI"
BASE COUNT         3215 a         1212 c         1186 g         2999 t
ORIGIN      
        1 ctaaatcttc tagttttata tatgtattgt tatagagaat atattttaaa atatattgat
       61 ttctttctat aggagaatct ggtatttcac ttgaatccat aatatcatca gatattaatg
      121 ctagatactg ttggtataat gctaaattaa gaatatctat ataatatcct tctccttttt
      181 ttattttaat aatagcacca tttttctcta aagttccatt tatagaagta atatcatttc
      241 ttatagttct ctcactcact tttaaatgat tacttaaagt tttacaagta attttatcat
      301 cactttcatt taataattta aacaacgtga gcaatctctt gtgcataaat atcccccctt
      361 atagaatttc ttctaaatta tactatttac actcatataa tacaattact ctaataaaat
      421 tgtattataa taatttattt ccacttctat atggaaatta gttattatca taatataaat
      481 ttccatgtcg aagtggaaat tagttatatt taatttaatt aggataatga ttacaatata
      541 aatataagtt aagacattat gaatggagtg gaaaaatata tggtactaca agaaaaatat
      601 atatttatag ataaagactt aaaaagcaaa gaagaagtat taaattttat tgcagatagt
      661 gcttatatac taggtattac aactaataaa gaggctcttt taaaagacct tctaaaaaga
      721 gaaaatgaat ttccaacagg aataaaagat ggatttgcga tacctcatac aaagagtgta
      781 aatgtaaagg aagcttccat actctatatt aaaacaacta acgagttaga gtggaaaact
      841 ttagatgata gtaaggttag atatgtattt agtttgttag ccccacataa aaatgaagga
      901 aatatacatt tgaaaactct atctaaatta gccacatgtt taatggagga tgattttaca
      961 agtgaagtta aaaatgctag tgatgcaagt catttagtaa aatatataac taataaaatg
     1021 aaggaggaat aattatgaaa atagtagcta taacatcatg tatagcagga ttagcacaca
     1081 cacctatggc agcaaaagct ttagaacaag ctgcatcaaa aatgggtcat gaaatcaaag
     1141 tagaacaaca aggtgctatg ggacgagtaa atactataac aaaagaagaa gcacaagatg
     1201 cggactttat acttatagca tctgaccaaa ctatatctgg aatggaaaga tttgatggta
     1261 aaaaagtgat aaaagtcaaa ataggtatag cgttaaaaaa accagaagct gtcttaacaa
     1321 agtgtataga agctatttct taataaaaaa atctcactct aatatagaat gagaaaaatc
     1381 aaaaattaaa aaattgtcta atgtaattat aacatattat caaaaaatat aacaaactta
     1441 tataggagga ttaaaaatga gtaaaaaaaa taaatctctt ttaagtgaac ttaagggaca
     1501 cttaatgaca ggtatctcat atatgttgcc acttgtaata ggctcatctt tagttgtagc
     1561 tatcccaaag cttacagctt tagcagtggg tataacaagt ttagatgctt ataaaaatgt
     1621 atctggtttt tatcatattc tttacttaat ggaacaagtt ggttggactg gcataggtct
     1681 attaaatacg gtattagcag gatttatagc attttcaata ggtgagaaac cagctatggg
     1741 tgcaggtttt attggtgggc tcatagcaag taatactaat gcaggattct taggagcagt
     1801 aatagcaggt ttctttgctg ggtatgtttg taagttttta aagaaaaaaa ttaaaatatc
     1861 tgggtcagca tcaggaatga tgccacttat aataatgcca cttataacag ttggattaac
     1921 tggtttttta atgagtgttg tattagcagg acctttagga aacataaata cttcattaaa
     1981 tgcttgggta gctgaaatgt gtcaaaatgg aacaaattcg gtggtattgg ctttaatttt
     2041 aggcgcaatg ataggattcg atttaggtgg accagtaaat aaagcagctt ggatggcagg
     2101 aaatgcatta ttgttagagg gaatctatct accagcgata ttggtaaact gcgcaatagt
     2161 tatacctcca ctaggatatg gtttagctac attcataaga aaatcaaaat tttccaatgc
     2221 attagctgaa acaggaaaag gcaatatagt tatgggtata attggaataa cagaaggtgc
     2281 aattcctttc actttaacaa atccattaaa attagttcct gttaatatga taggatgcgc
     2341 tttgggtgta ggattatcag ctttgttagg tgtacatgct ataatgcctc cagttggtgg
     2401 attatatgga tttatatcaa taggttctgg atgggcttac ttagtaggtg caatatttgg
     2461 agctttagta atagcaatat tatcaacaac tttagtagac ttcaatgaag atgaaactac
     2521 ggaaaataca gaatttgatg aaatagagtt agatataatg taaattaaaa acttaatttt
     2581 agataattga gaattgagtg tactctcaat tctcaattaa attttgggag gatttttatg
     2641 aaaaagacaa aagtacatgt agttccacac agtcattggg atagagaatg gtattttaca
     2701 acatcaaggt caaaaatata tttaatgaaa gattttcaag atatattaga catattggaa
     2761 gaaaaagaag actttaaata ctttactatg gatgcacaag cttctctctt agatgattat
     2821 ttaaaatgga gacctcagga taaatataga ataagagatt tagttcaaag aagaagatta
     2881 gttattggac cttggtatac tcaaacagac caattggtta tatcaggaga atctataatt
     2941 agaaaccttt actatggaat agatagatgc aatgaatttg gtgaacctat gagggttgga
     3001 tatgtaccag attcttttgg gcaatcagct caaatgccac aaatatacag aggttttgga
     3061 atagatagtt cattattttg gcgtggagtt tctgatgata tggtagatac aacagagttt
     3121 atatggaaag gttctgatgg ttcaaaagta ttagcagttc aaataccatt tggttattac
     3181 tatggaggta atataccaga agatgattca gatttaaaag aatatttaaa agatataatc
     3241 gtaaaactta aggttaaagc ttctactaat aacgtatatt ttccaaatgg atttgaccaa
     3301 gcaccaataa gaaaaaacct tcctgatata ttaaaaaaag ctaatgaaat agataatgaa
     3361 aatgaatacg aaataagtga tatagaaaac tatatagatt cagtaagaaa tgaaagaaat
     3421 gaatttacag agcttaaagg tgaattttta aatgctaaac atatgagaat acacaaatcc
     3481 atattctcct caaggtcaga ccttaaaata atgaataaca aattagaaaa ttatattaca
     3541 aatgtacttg aaccgttatt aacattaagt tattcacttg gaaatgaata ccctcaccta
     3601 gttataaaag atatatggaa acttatgttt gaaaatgcag ctcatgacag cataggttct
     3661 tgtaatagtg acacaacaaa tgaggatgta tacacaagat acaaacaagc cagagatttg
     3721 agtataaatt tattagattt gcatatgaga cttataagta ctcaaatcaa aaataataat
     3781 gaagaaataa cattaacagt attcaatgga ttcccagaag tcagaaatga agttgtagaa
     3841 tttgatactt atattccagg tcaagatttt gtgataaaga ataaaaatgg aaaaattctt
     3901 aattatataa taaaagaaaa agaagatatt acaaattata tattaactca aacaataaga
     3961 ttaaacccaa gtaaaaaaat atatataccc aaaactgtat atagagcaaa aattgcactt
     4021 tcagtaaaag atataccacc aatgggatat actcaactag tttttgaatt ggatactaaa
     4081 aataaatgtg aaatagaaac aagtaattgc aaggaaatag aaaacaaata ctacaaaata
     4141 acagccaata aaaatggttc attagaaata ttagataaag aatctaataa gacttataaa
     4201 aaccaagctg taattgaaga caatggagat gatggagatt catacaacta ttctcctcca
     4261 agaaaagata tttatgtttc atctttagat tctgtatctt cagtagaagt gctaaaatca
     4321 aacattcaag aagaaatgat acttaaatat tcattgacca taccagcttc actggaagaa
     4381 agggctatag gtaaagtttc tgtgaaaatg ccagtaacaa tgaaaatcac cttagaacct
     4441 aaagaacaaa taattaagtt taatgttaat ataaaaaata accaagcact atctcataga
     4501 gtaagagttt tatttaatac agaaatagca tctaagtttt ctatagcaga tcaacaattt
     4561 ggaataatac aaagaccaac agtattagaa aaagaccttg cattgtggaa aagagacaat
     4621 tactcatggc aagaaaaacc tataactatc gaaccaatgc aaagttttgt tactttagag
     4681 gatggacaaa gaggaatagc attaataact ggttgtgtaa gagaatatca aatagttggt
     4741 gataatttag atacaatagc attaacacta ttcaggtcat ttggatatat gggaagagaa
     4801 aatttacttt atagaccagg aagagcttct ggagaaaaaa taatagcaac accagatgca
     4861 caattactta aagagttaaa ctttgacttt ggtctttaca tatataacaa taaatttgat
     4921 gaagcaaatg tagctaatac agctaagaga ttcttaacac ctatacaaat atatgaatat
     4981 gctgattttt taaatggtag acttatattt gcattcaatg acgaaaaaca aatatatgaa
     5041 aatgaattta gtttgatgaa tgtaaataat tcaaatttca cagtaagtgc aataaagaaa
     5101 gaagaaaaag gagatggaat tgtagttaga atcttcaatg gtatgaaaaa tgaggatgct
     5161 aagggaagtc ttaacatcaa taaaaatgtc aatgatgctt atagtgcaat gctcgacgaa
     5221 atctatgata atactagcaa gcttaaagta aatactaaaa ctgtagaggt aaattcttta
     5281 tcacattgta aagtacaaac aatagtaatt aaataatata aaatagctta tacaagattt
     5341 caaaataaat tgctagtacc atgttaatag taaagccaga tattatgcta tttataagct
     5401 ctgtaacact aaataacatt ttgtaaaata aaaaggtgaa ccttgtatat taggattcac
     5461 cttttttaaa taaatcaaca ttctgtttta aaataacatt tataagctgt tttgaaataa
     5521 tgcttacaaa ttattttaaa acaataccta taaattttta tttaaagaat acacataaat
     5581 atttccagac tcttttatcc tatagacttt ataattatta aagaagtcta atctaatttt
     5641 tgttaaaagt aattgtaact ttaaataaat caccatccac acttatattc atcttaccac
     5701 cctgtaattc tataaaactt tttgctatcg ctaaaccaag accagaacct tctgtgttac
     5761 gtgatttatc tcctcttaca aatctctcca ttatatcatc tacactaaaa tcaatctcag
     5821 atgcagacat atttttaagc ataatattta ctttattatc tgtttctaaa atatcaatat
     5881 acactcttga atttggcatt gcatatttgc ttatatttat tataagattt tcaaatactc
     5941 taaatgtacg ctgactatct aatggtaata ttattttttc attagaaaag ttattcttta
     6001 ctattagaga tgaatctgca agtttgtcat ctaattctaa aagtgtctgc ttcattagag
     6061 atacaatatc cacatttact atattcaaat aaacatctcc actatttgcc ttgcttactt
     6121 caaacaaatc ttctattaga tgttgcaatc tttgtgactt tctgtctaat gtatcaatat
     6181 acaacttacg attttcatct gttatacttt catcttttaa taaatctata taagtgatta
     6241 tagatgttag aggtgttttt aagtcatgtg atacatttga tataagctct gttttcatct
     6301 tttgactctt aacctcttcg tcaacagctt ttttaaatcc agtttgaata tttcctaatt
     6361 cgtcttttat aggattaaaa aatcctaaat ctttttccat atttacatct aagtttccat
     6421 ctgaaatttt ctttgtaaca tctaaaagtt gattatattc atatattgtc tttttagagt
     6481 aatctgacag tattttaaat aatactactg aatatattaa tgcgagtatt attccaaaga
     6541 accacatact acacattata actataatca ataaatttat tcccaaaatt gtaactatag
     6601 ttttatttgg cttttctttt agatttattg ttctaatttg attaataact gtttttcctg
     6661 ctttttttag ccatacaaaa aacattcctg tcacagtatg ttttgtaaaa tactctctta
     6721 ttccaacatt taaaatatgt tttaaaagta ttataaatac aaatattatt gagaatatta
     6781 caaaccaata agttaaattc attaaactta ccaatatact agccatttta gcacttatgt
     6841 cattgtttat aaggtactct acataattcc cactcaaggt attacttatt ataacagtag
     6901 gatataaatc tacaagcccc tgtataatag caagagcaat aaagagtatc tctaacggta
     6961 ttcttgataa tgtatcaaat cctagcaact ctttagattt tctatatggg aatgctaaag
     7021 ctacaataac aactatagct aatataattt ttaaaatcat tccagaaatc ttataataag
     7081 aattattttc tacattgctt atatatgaaa ttataccatc atcaaaataa gtatttccat
     7141 aaccatactc tacactatca cctttactta ttactttagg tacagcatat actaatgtta
     7201 tattttttat tggagataac ttatatgaaa ttgaatttcc tcctaaactt gtatccatta
     7261 aatcttctct tgctttagat aacaacattt gattaataat tttttgattt actccataag
     7321 aatccacaat atttactttt ccatctttat caaaattcat tacaacgtag aatctatagt
     7381 tttcttttat atctgtaatc tgcttttgtc tttcctcatc attcatttct gtattttttg
     7441 actgattttc atcagaattt tcttttgaag catcattttt tgttgtatca agtactccta
     7501 attctttatt atcattaaaa aattcaaaat tgctttcttt atcaactaaa tagtacttta
     7561 aattctttcc tgacgcttta atgaaatgct cccaattttc aaagttttca ttaaaatctg
     7621 cattaatact atcatctgta tagtaagttt ctgaaccatc ataagtatct ttttctttac
     7681 ttacattttt tatatagttc atagtttctt cattgataaa tagttttgat ggttgtatca
     7741 tcttaccttc ttgttcttcc ttcatattat gatataaaac ataacttcct ttatatatac
     7801 tatctacaaa gctttcctgt tcaaatggat tctctgctcc acctttagca cttgcttgta
     7861 tagtagaata ttgactaatc atacctaatg atgctgataa cataagtata aaaactataa
     7921 tagtaactat atatttatct ttaaattttt tcaattttgt atccaacacc ccacaccacc
     7981 tttaaatatt taggattttt tgggtcaatt tctatctttt cccttatatt cctcacatgt
     8041 accataactg tatctgtatt tacagcattc tcattccaaa ctctttcata tatttcatca
     8101 gcagaaaaaa ctcttcctgc atttctcata agtaaactta gtattttaaa ttctatagga
     8161 gttgttttta tatactttcc atcaatacta acttcttttg tattttcatt taattctaat
     8221 ccgcctattg caaacacact atcgtcactt tctacctttt gctctttatt tccaaccatg
     8281 tttagatact tagtgtacct tctaagttgt gaattaactc tagccaatag ttctaatggc
     8341 ttaaaaggtt ttgttacata atcgtctgct cctatattaa gccccattat tttgtcaact
     8401 tcctctgatt ttgcagatag cattattact ggaaaattat agttttctct aagcttcatt
     8461 gtaagtgtta taccatccat tttaggcatc attatatcaa ctatagctaa atgtatttct
     8521 tggttttcta taaccttaag accttctaat ccattgtatc ccttaaaaac attatatcct
     8581 tgatttaata gatatatttc tatagcatct gc
//
LOCUS       DAGSHT010000143         8470 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGSHT010000143 DAGSHT010000000
VERSION     DAGSHT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.148"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(8..238)
                     /gene="dltC"
                     /locus_tag="MJB72_002769"
     CDS             complement(8..238)
                     /gene="dltC"
                     /locus_tag="MJB72_002769"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBY8263845.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(257..1405)
                     /gene="dltB"
                     /locus_tag="MJB72_002770"
     CDS             complement(257..1405)
                     /gene="dltB"
                     /locus_tag="MJB72_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBY8263846.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1405..2919)
                     /locus_tag="MJB72_002771"
     CDS             complement(1405..2919)
                     /locus_tag="MJB72_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBY8263847.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYRELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3040..4230)
                     /gene="dltD"
                     /locus_tag="MJB72_002772"
     CDS             complement(3040..4230)
                     /gene="dltD"
                     /locus_tag="MJB72_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBY8263848.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(4518..5537)
                     /locus_tag="MJB72_002773"
     CDS             complement(4518..5537)
                     /locus_tag="MJB72_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY8263849.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(5713..6252)
                     /locus_tag="MJB72_002774"
     CDS             complement(5713..6252)
                     /locus_tag="MJB72_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBY8263850.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(6353..7033)
                     /locus_tag="MJB72_002775"
     CDS             complement(6353..7033)
                     /locus_tag="MJB72_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBY8263851.1"
                     /translation="MQNVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(7062..8351)
                     /locus_tag="MJB72_002776"
     CDS             complement(7062..8351)
                     /locus_tag="MJB72_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBY8263852.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         2788 a         1510 c          822 g         3350 t
ORIGIN      
        1 tactaattta ttttcttgtc tccaagaatt ttactaaatt atttacagta gacatatctt
       61 ttctctctaa atcagttggt tgaagctcta tccctaatct attttcaatt tcaagtaaaa
      121 cttcaattat agctaatgag tctaaaagtt ccgtctcaaa taaattaagg tctaaatctt
      181 ctcttatctc atcagtccct aatacatctt caaatatttc tataactgtt tcttgcataa
      241 attttcctcc ttaaaattaa gctaaataac ctgagaatat cagcaatcca aaacacacaa
      301 tatggaatgt aatgaaaatc tgcactctct cgaaccattt atctttttta tgttttttat
      361 aaaatttaga ttttcgctga tatatatcag ttaacacaag tgccaatcct tggtatacac
      421 cataagcaac ataataccaa gttagaccat gccaaaatcc cattgttatc atggttatca
      481 tctgagccac atgagcagca gtagtccttt tcttaaatct tttctttctc attgatgaca
      541 taacaaatct agaaaaaata taatctccaa accatcttga aagacttata tgccatcttg
      601 tccagaactc tttcatatct ttgctgataa atggcttgtc aaagtttata ggtacttgta
      661 ttccaaatat gtaccctgta cctatagcaa ataaactata tcccgcaaaa tcaaagaaca
      721 ggtataaact atatgcgtac atataactta aaatatttat aaaactcata tctttaggta
      781 ttcttgatac ccaatatgta tttatcaaaa atgctataac aaacttataa cctactccca
      841 tgacaatatt ttttattcct ggcagtaagt attcttctat atactctttt ctagagattt
      901 gtttttccaa gtcctgttca aatcttcttg acctatcaat aggtccagaa cttaaagttg
      961 gaaaaaataa cataaaataa agcatctttg aaattttaac ttcttttata gctccatcat
     1021 aaatttcaat aaccatttgt atagttctga aatttaagta tgatattcca ataaatccaa
     1081 ttattccaaa agaagtaact ggagatattt tatttattat tattgggagc attgatgcaa
     1141 acaagaatcc ccaatatatg tatttatttt tagtttttct tcttacatat tcatatcctt
     1201 taactattat tacttcaaga ataataaata ttaccaagta ctttagttgg acatcaattc
     1261 ctactattaa gaatatcata aacaaacttg ccaacatacc ataatacttt atattcttgc
     1321 ccattagacc taaaattact gctggtattg atgttaataa taatatataa agataaaaat
     1381 aatctccata ttgtgaaaaa atcattaaat ttcctccata agtttctttc tgtctatttt
     1441 accattgata tttgttggaa actcagagat tattgaaata tttcttggaa tcatatatga
     1501 aggtatatac tttccaagtt ccttttttat tatcatccca tttttaatat tacttaaatc
     1561 attcttttca tttaactcaa caataccttt taagtaagca attttttcgt ccttatatac
     1621 tggtaaaact actgcatttt ttacattatg tacttttctc aaattatttt ctatatcttc
     1681 tatttcaatt ctaaatccat ttaatttaat ctgaaaatct tttcttccac aataatatat
     1741 atttccatct aaaagatatc ccatatctcc agttttataa gctctccact ttacaccatc
     1801 tatttcatca tagaaaaata cttcatcagt cttttcttta ttattgaagt aacctttgga
     1861 aactgatggt ccaattatta taatttcacc cttttcattt tctttaagct catttccatc
     1921 ttcatcaagt atttttattt tacaatttga catagggtat ccaactggaa gacttttttc
     1981 atcatctata gccttttgag tcatatcatt tacacttaca ccaacagtag cctcggttgg
     2041 tccatatcca ttgataattc ttgtgtttgg aaatctactc ataagttctt tagtaagatt
     2101 ttttgaaagt gcttctccta tgaatatcat tgactcaagg ttaggaagca ttttactatt
     2161 aaactctttt tcagttatac atactcctgc aaaagaagga gttgagaccc atacagctat
     2221 atctgagata ctaaattgtc taaatagttc tctatagtct gctaatacat cttttgatat
     2281 tgaaaataat gtagctccat ggataagacc agggtatatt gttgtaactg aaaggtcaaa
     2341 agaatatgct ggttggttca ttattacttt ttcacttcca tctatgttta gatatgggct
     2401 tatccaatct gaaaagctat ctaaattgtt tgaacttatt tgaacccctt ttggctttcc
     2461 tgtacttcct gaagtaaata gtatatatgc attttcatca tcttttaccc agttttcttt
     2521 atctaatgat tttccctgat attcatttat tatatcattt aatttatcca tatcaattac
     2581 attgatatct ccaaaatttc tttcatcact aaagttaaat aatacctttg gttttatctc
     2641 tttagtgact tcaaaaactc tatcaattgg aaaactaata tcaagaggca catacgctct
     2701 acctgatttt aaagctccta tcatacatgc cattatcatg ttttctttat ttccataaat
     2761 tactatagga gtatcttctt ctttgtatac atcctttaga aatactgata tagcatctga
     2821 atattcattt aaatctttat aagaaagttt atctccatta cacattagtg ctgttctatc
     2881 tgtatttgaa tattttttta ttccttcaat aattttcata taagaatgct cctttaaaat
     2941 ccgttataaa taaatggcaa ttctttaaat ggagtaaatg ttagaactcc tatcgccaat
     3001 attataacca tagttaaaca aatactagtt aacaaatctt tattgttctt taaatatttt
     3061 aaataatctc tcacagacat ctacccatcc tttcgtacca agatgcatta catcccttaa
     3121 ataatactta tctgaccctt tatctttaag gtttagaact tcaaagcctt tgctttctgc
     3181 tattttttgc gctttatcgt agtactgatt tctttctttt tcagaaatac cagtcaaatt
     3241 atagaattta tccatacttg ggattaatac aacaactggc ttaataccta agtcagtaca
     3301 tacatttagc atcagtttat aattttcaaa ctccttagat gttaataaat ttacatcttt
     3361 gtctcttcct ttgtattgct ctataccatc tttaaaatgc tctttataat agtacttatc
     3421 aatatttagt ggatttttac caactctttt tttagcatct tcaatggctt tctttctctc
     3481 atatgaccaa tttattggtc tttttctttt tgttgctctc tttttatcta gcttaattaa
     3541 tcgtttataa agaataccct tttctttaag agctatacaa tactttcttc cttgaaaata
     3601 aggctctaaa agcacctttt cagctttttc aggaaatgtc ttaggctcat atagcttagc
     3661 atataatgct tctgctttat actcgtcact tccccataat aacttacttg atttttttgc
     3721 atattcaatc ttattttgtt tagatatttt tggattatct aaaaatcgat aaaattgtat
     3781 tggtgaaaat ctagactgat aatggtgact tgtgactccg tctttttcca taaaccattg
     3841 catagaaatt aacaagacaa ctttcttatt atcaatatta ggattcatac ttcctaatat
     3901 tgcagcatct tgtaatgtct gagtataggc tctcccaatt gcaaacacct tatttttact
     3961 cctatttgta ttaaaatagt actttggatg ttgttttgtt gaattactaa gttctgatga
     4021 ccccaaaatc ataatatcct tttctcttaa aaaatgatta tttgctgtaa cccctttatc
     4081 ttttatatca cttaaagtat catccataat tggaagtaga tttttctccc tcaaaagttc
     4141 atcagtttta gaatcaagaa atttgtctaa cccaaataaa aatactacgc ctattataaa
     4201 aggtgtaata aagtatatca attttctcat attttctctc ctctaaaaat attcaaattt
     4261 atgttattat taatcatatt agttttgata tgtatattca actacaaaat tgtaataaaa
     4321 tcgtcaaaac tttttttgat actgttacaa aattgtaaaa aaaaagaaaa aaacgccaaa
     4381 aataaatttc tttttagcac tttttcatac ttataatttg acattttaac acttggttaa
     4441 gtatacacct taccttaact taaaattaaa tttatttaag aatatatatt ataaattctt
     4501 atattttcta atataaacta tatactatca gctggataaa caatactact ttgtcttctt
     4561 atctcatcca taatttccat tactattatt gaattttcat gagaatttat atttgattct
     4621 attttattgt tatttataag acttataaac tctacaagtt cataatacat tccttctatt
     4681 tctctaggct tttcctctgc tttttctttt ggaatagata tatcttcttc tcttccatct
     4741 ctgtatttaa taatagcttt ttcaaataga ttaagctttt caattattat acttccctct
     4801 tctccttgta tttcagatgg tatataagaa tcagctactt tagaatactg aattatcgca
     4861 tccatatcat catattgaaa tacagctgaa ccttctccat ccactccagt ttgaagtata
     4921 tgagaaactg cctttacagt ttttggagca ccaaataaat tcaccatagg atgtatacaa
     4981 tacactccta tatccattaa agctccattt gacaactctt ttttaaaggc attttctata
     5041 attccatttt tatatttatc atatcttgaa gaatactgac aatagcttcc aaaaaatctt
     5101 cttattttcc ctattttata taagttttct ttaactgctt taaagtttgg aacacatgta
     5161 attctcatag cttccataag caatacatta ttatctttgg ctgtttttat cattgatttg
     5221 acttctttta aattagatgc taacgacttt tcacaaagca catgtttttt gtgttttaaa
     5281 caagtaatgg tttgctttga atgtaatgca tttggagaag ctatgtatac agcatctata
     5341 tccttcgatt tagccatttg ttctaaatca tcaaaaaaaa tacttgctcc atatagagtt
     5401 ccaaattctc tagctttttc taaatttcta gaatatacag caaccaattc aaagtcttta
     5461 accctagatg cagctcttag aaatatattt gctatattgc ttgtcccaac aataccaaat
     5521 cttattttac ccatcataaa tcactcccca ttttctacac tttattttaa ttttaataaa
     5581 aaactttaca acttatagat atattataaa ataattttat caaataaaaa agaaagctaa
     5641 ttttaaatga tttcaccatt ttaacatagc tttcttttaa ctacaatcta aattcaactt
     5701 atatttttaa agttatcttt ctactatatc tttatttccc ctcaaaaatt ttatagtaag
     5761 aatttcatcc acaaacaatg ttactaaaag tatagttaca acaattggcc aataaggtat
     5821 tataagacta tataaactat caactatata cacttgaaaa tatctaactc caataaatgt
     5881 caatatagtc caatatacag aaaactttag acatactatt cctctactat tgtacttata
     5941 atctgaataa tcccaataga ccttattaaa atatactttc ataagtattc ctgtaatata
     6001 ttctactact gtcggcacta ctagacataa taaaagcaat gcaaatatat tattggggtc
     6061 aataaatctt gagacctcta ttataatcga cattgcaatt gcatacattg gtttaaatgg
     6121 accatttaaa aatccatctt cttgaaaatg cccttgttta taataactat ataagttttc
     6181 aattatccaa cctataaaac catacaagaa aaaattaaat aaaaaatata atagagaacc
     6241 tacattatcc atgaatattc ctcctagctg tttatactta ttttttctca tattcatgga
     6301 tttattctaa atcaaagaaa ttatatttaa tttatttaat taatacttta tattatagat
     6361 tgtgacattc tttagagttc aaacttttta ggtattctct aacttccagt aaattttcaa
     6421 gtatcttatg tgattgtgat ttaatttgct catctggctc ttttaaatct ggtacattta
     6481 tacatcttat accaccattg tatgcagctt caactcccat aggcgaatcc tctatgacaa
     6541 tacaattttt gggattaaca tttatctttt tagctgcttt taagaatatc tcagggtttg
     6601 gtttagagtt tacaacatca tctccacaaa ctatagcatc aaagtaatct tttaaatttg
     6661 ccttagctaa tctctttact gctctttctc tctttgttga tgttgcaaca gccattttgt
     6721 atccattttc ctttaaaaaa gagattagtt cattgactcc taatttgata ggagctccct
     6781 tcctctccat aaattcaatc atatttttag ttttttcgtc atataaatct attattggta
     6841 cagaactatc gtatatatct gtaagacctt ctattattcc tttacgattt cttcccataa
     6901 ctgatgtata aatttcctta gtcatagtat atccatactt ttcaaacgtc tccatccaaa
     6961 attcaagaga aattctctct gagtcaaaca aaactccatc catgtcaaaa ataattcctt
     7021 ctacattttg catacaattc ctccaatatt ttttgatata attatattac actttttatg
     7081 attaatttta caaccctcaa tttatccaat tctaatattg taaaattgta attttcaaaa
     7141 gttacacttt ggtttacttt aatatttgaa ccaagcatat aggatatcca tccacctata
     7201 ctgtcaaatc catcatgctc tatatcaagt ccaaaatact tattaatata gttgatagat
     7261 tcagttccat caactaagta agaacctgct cctattttct taatatgagt ctcttctttg
     7321 tcaaattcat cttgaatttc tccaactatc tcttctagta tatcttctat tgttaccaca
     7381 ccagatgtac caccatattc atccacaact atagcaagtt gaagtttttc ctttcttatt
     7441 ctctcaagag ctttatctat agttaaattt tcagatatat atataatatc tctcaatatt
     7501 tcttctagtt caattttatt ttcatttatc ttttgattgt ataaatctct tatatgtaca
     7561 aaacctaata tatcatcttt attttttctg catactggat atcttgtaac accttcctct
     7621 tttaaaatag ctaatatctt ttcttcagaa tcactttcat atatacatac catatcagtt
     7681 ctaggaacca ttatctctct aattttcttt tcttcaaatt caaaaatatt atcaactaat
     7741 ctttgctctg attcatctat aagtccactt ttataacttt cttctactaa tagtcttatt
     7801 tcgtcacctg tatggggttc atcaacctca tcagcttgtg aatatccaaa tggtttaaga
     7861 aataaatcag tacttaagtt gaaaaaatat attattggat atgttaactt ataaaatcct
     7921 actaataaaa aagatgtact caacattatt ttttctgtat tataaagtgc taaagcttta
     7981 ggaaccaact ctcctattac aacctccatc attgttataa ttaaaaaaga aatggaaatt
     8041 gatatagtat atataatact ttcactaaga ttaaatacac ttactattgg taaaattaac
     8101 tctttaactg tagattctcc catccaacct aatgcaagtg aacaaagtgt tataccaagt
     8161 tgacatgcag aaagatagga atttaagttg tcttttatta ttttacatct ttttgcatta
     8221 ttattacctt cagttatcat ggtatcaatc ctagaactac gaagttttac catagcaaat
     8281 tcacaagcta caaaaaaagc atttatcaaa acaagcatta ctataagtgt tacttttaat
     8341 aactcccgca taaagtattg gttcttataa tgtaagaaat ctataagaac tcaaagttcc
     8401 ccccttatat taataaattt aatttataat atatattcta tcatatttat taatatatcc
     8461 tactttatta
//
LOCUS       DAGSHT010000144         8409 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGSHT010000144 DAGSHT010000000
VERSION     DAGSHT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.149"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..404
                     /locus_tag="MJB72_002777"
     CDS             <1..404
                     /locus_tag="MJB72_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8263853.1"
                     /translation="SKYFISKKIILDLNNLNFKVLSDEKWLYFIFEQLLSNSLKYSSL
                     GDNISIYCKRTKNYLQLRVEDTGCGIKKEDLNRIFNKGFTGNNGRNNSKSTGLGLYLV
                     KELCDKLGHKIDVDSEYNQYTKFTITFKCDL"
     gene            516..1745
                     /locus_tag="MJB72_002778"
     CDS             516..1745
                     /locus_tag="MJB72_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBY8263854.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            1753..2427
                     /locus_tag="MJB72_002779"
     CDS             1753..2427
                     /locus_tag="MJB72_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8263855.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            2430..3662
                     /locus_tag="MJB72_002780"
     CDS             2430..3662
                     /locus_tag="MJB72_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8263856.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(3948..5213)
                     /locus_tag="MJB72_002781"
     CDS             complement(3948..5213)
                     /locus_tag="MJB72_002781"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBY8263857.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(5266..6414)
                     /locus_tag="MJB72_002782"
     CDS             complement(5266..6414)
                     /locus_tag="MJB72_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBY8263858.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(6514..7059)
                     /locus_tag="MJB72_002783"
     CDS             complement(6514..7059)
                     /locus_tag="MJB72_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBY8263859.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(7282..8226)
                     /locus_tag="MJB72_002784"
     CDS             complement(7282..8226)
                     /locus_tag="MJB72_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY8263860.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         2914 a         1387 c         1104 g         3004 t
ORIGIN      
        1 actcaaaata ttttatttca aaaaaaatta ttttagactt aaataatttg aattttaagg
       61 ttttatctga tgaaaaatgg ctatacttca tattcgagca attattgagt aattccctaa
      121 aatactcctc attaggagat aatatatcta tctattgcaa aagaaccaaa aattatttac
      181 aattaagagt ggaagatact ggttgtggta taaaaaagga agatttaaat cgaatattca
      241 ataagggatt tacaggtaat aatggaagaa acaattctaa atctacaggt cttgggcttt
      301 atttagtaaa agaactttgt gataagcttg gtcataaaat tgatgtagat tcagagtaca
      361 accaatatac aaaatttact attacattca aatgtgattt atagtatctt attattgata
      421 gtttattttc attaacttat attcaacttc ttatagtaat tctatatgta catcccagta
      481 tataattaag tagtttttat aaaaaggagg aagacatgtc ttttctaaaa aaatttaaaa
      541 ataaaagatt tagaaataac agttttaaaa gtaacaactt tagaaatagc agttttggaa
      601 gtagtggctt caagtctaaa aataaaggtt ttaaaaacaa tccctttaaa aataagagat
      661 tcaaaatata tgcaggaata gcagtaatca tacttttaat tctaggagtt cttgcttatg
      721 ttgattcgaa aaacactgaa ttacaagcta atgaaaattt tatcgaaaca tatactattc
      781 ctgaaaatga aaaaatattt ataaatggga tggttgttcc taagcaaact aaggacttca
      841 atatatctgg agattatgag ttaagtgatg ttaatgttac aaatggtcaa aaagtaaatc
      901 aaggagacct tctttttact gctaaaaatc caactataat agcagaaata gatagtttaa
      961 aatcacaact tagtcaatac aaaaaacaaa aaatatcact ttctgatatt gctgaaaata
     1021 aagatgctat tgcttctata aatgcacaga taactgcttt aaactctcag atagcttctc
     1081 tggataaaaa agcttatgac agagttactg ctccttttga tggcacagta tacttgaatg
     1141 accaaactgg aaatccagac caaccagttt catttatgac tattcaaggt ctagagtttt
     1201 atatgaaagg acaagcaagt gaacaagatt tacctaaaat gaagattgac caaatggtca
     1261 atatactagt tttttcaact gaccaaaagc ttactggtag aataagtttt atatctgata
     1321 aaccatctac tccaaatact gaaatgggag ctcagcagaa tacactatct tattatgata
     1381 taaacatagc atttgataat caagaaggat tagtaaatgg attccatctt caagcatcac
     1441 ttgaagtagc taattcaagc tttaaaatac cagcatcttg tgtacttaaa gacaacaaac
     1501 actcatatgt atttaaggat ttagatggta tattgaaaaa gcaaattgta gatgtagcaa
     1561 gtcaaaatga tgattttgct gtagttagag gcggtcttga acaaggcgat ataataataa
     1621 aacaccctac taaagaaatg aaagaaggag acccagttca aggcgatggc gtaactgctg
     1681 gttctaataa tggagatact actccaaaca aaaaagtaga agaagttact atggatgtga
     1741 attaatttac atatgttaat taaattagaa aatattcaaa aatattacaa agtagggaaa
     1801 gatgaattac atgttttaaa atccttaaat ttagaaattg aatctggaga atttgtcatg
     1861 ataatgggta agtctggtag tggtaaaact actttactta atatcttagg ttttctagat
     1921 gtatttgatg aaggaagata tatatttgat ggaacagatg taactaattt gagtgaaaat
     1981 gaacgttctg tttttagaaa tattaatatt ggatttgttt tccaacaatt taatttaata
     2041 gaaactctaa atgtatatca aaatgtagag ttgcctctaa tttacaataa agcactaaaa
     2101 aaatctaata gagaagaaat tgttaaagat aaattaagtt ctgttgggct tttggacaag
     2161 cttaagcaga aaccacttca attatctggt ggtcaacaac agcgtgtagc cattgcacgt
     2221 tgcctagcaa atgaccctca gataatcttt gctgacgaac cgactggggc attagatagt
     2281 gaaactagca gagaaattat ggagctttta acaaggctca acaaacaagg taagactata
     2341 ataatggtta cacatgatca agatttaact aagtatgcaa ctaaggttat acgacttaaa
     2401 gatggtgtgt ttacttcgga ggtgtaatca tgagtttatt aaaaaattct ttggccaatt
     2461 taaaaggtca caagcttcgt gtatttgtag ctttactctg gattataata ggaataactt
     2521 cagtaatact tgttagttct ataggtaatg gatttcaaaa agaaattaag aaatctgtaa
     2581 acaatgtaaa ccctaataaa actactattt cgtttgaatc agccgataac acaggtctta
     2641 ctgatgatat gagtattttt ttaaaaccct ttaatgcaaa agatttagaa gaattatcat
     2701 ttgttgaagg agtagaaaga atagctcctt caagagatgg gtttaacctt gattcagttt
     2761 actcttcaca agcatctttt gataaaaaaa ctacgtatgt tgatgttgga ccagtaaaaa
     2821 aagattctaa aataaattta atttgtggta gagatttttc tttagatgat gaaaaaagaa
     2881 aagtaatttt acttacatta caaagtacaa gtgaaatttt tgaaaatcca gaagatgcct
     2941 taggtcatgg tatcaacata aatggaacta tatttgaaat aataggggta ttagatgatt
     3001 ctcagcaaaa ccaagctgga ggattctttg gaggttacca agatatgcaa tttactactt
     3061 ccctagtacc taaaaaggct tttgacactt taatgagtca aaattcttat tcaaatgaaa
     3121 tttaccagtt agacttagtt tcttctaaag gctataatgt aaatgaagtt gcaaacaatg
     3181 ttatagctaa attatatgag atgcaccctg gtatcaatgg ttcttatact acaccagacc
     3241 caactgaaca gacagcttat cttgaaagca ttaattccaa tgtaaacaaa tatgtatcta
     3301 taataactgt agttgcaatg tttgttggtg gtattggtgt aatgaatata atgtatgtat
     3361 ctgttatgga aagacaaaga gaaattggta taagacgtgc tattggagca aaacctaggt
     3421 ctatattatt tcaattttta gttgaagctg tttttataac agtatgtggt ggaattttag
     3481 gaactatagt tggatttgct gctactaact atgtttctaa atatataggt tttgaagcta
     3541 tacctagctt aaatagctta ttttatgcaa ttgtagctac tatattgact ggagttgtat
     3601 ttggtctgat tccagccttt aaagcttcta aactagaccc tataaaggca atttataagt
     3661 agaaattgta taaagatttt cgatttttag attagtttat ataaaacata cttaaatata
     3721 taacgcacta ttatatatga tagattagga acttaatctt tccaaatata atagtgcgtt
     3781 ctttcataaa aaggattctc ttccttacta tatctaaatt accaagatgt tatgttctaa
     3841 ttgttcaaac taatacttcc aattttgatt aatcaatctc attctcctaa tatacaatct
     3901 tttactttca atcattgtac actccgcctt aaaaaaataa tcttaaatta ggcgttatat
     3961 acaatctccc cattacatat tgtataatta acctttccat ataaagattt acctttaaat
     4021 ggtgaattat cagctttaga aacaaagctt tcaactgtcc attcttcttc tggattgaat
     4081 ataacaatgt ctgctacagc cccttcttct ataaatccaa tatttaaatt gtacaacttt
     4141 gcaggattta tagacatttt ttctattaac tgcatcatac taagatgatt tttatgcacc
     4201 aaatttgtta ttccaagagc taaagaagtt tcaagcccaa taattccact tggagctttt
     4261 ataaactctc tatctttttc ttctttacta tgaggtgcat ggtccgttgc tattatttca
     4321 atagtaccat catttaatcc ctctataatc ttatctctgt cccatttact tcttagaggt
     4381 ggattcattt ttgcatttgt tccaaactcc aatacatctt cctcagtcaa tgtaaagtga
     4441 tgtggtgaag cttctgcaac tacatttgca cctaagtatt ttgcaaatct aaccatatca
     4501 acagatacac ctgaacttat atgttgtata tcaactttag caccagtctt tagtgccaac
     4561 atacaatctc tggctaccat aacatcttct gctacacttg atgcaccttt taatcctagt
     4621 tctgaagcta cctttcctgc atttacacct ggatttccaa ctaaagaagg gtcttcttca
     4681 tgaaaactaa gtggtacatc caactctttt gccttaatca ttgcctccat aattagattg
     4741 ctatccatta aaggtaatcc atcatcagta aaccctggta caccagcttt tttaagagct
     4801 tccatatcaa ctaactcttt accttcaaaa cctttagtta tagctgctgc ttgtaaaaca
     4861 tttatacatg cattttttga cttttcttta acataattaa aagtatcttc attatctaca
     4921 ataggattag tatttgccat acatataaca gttgtaaatc cacctctagc agcagattta
     4981 gctccacttt ctatatcttc tttatatgta aacccagggt ctctaaagtg tacatgtaca
     5041 tccactaaac ctggagcaac tacattacca ctagcatcta ttattttttc atattcatca
     5101 ctttcatcaa acttgcctat tttataaatt ttattttctt ttataattaa gtcaatattc
     5161 tcatctcttt gacttttagg gtctattact ctaccatttt taattaaaat catattattt
     5221 ctcctttata tttttaattt atatagactc atataatatt atacattatt ttacatactt
     5281 aaatacaaaa ttacgtttga catcaatagc ctttttagga caaagttcat ggcaacagta
     5341 acatcttata catttactta agtctataat agcaccctta gtccctatct ctatagtgga
     5401 tgcaggacaa gcttccttgc attttccaca ttttatgcaa tcttgaaatc ttaccacagg
     5461 ctttggtgtt ataagctttg aaataggttc atttaaaaat tttggtagct ttacggaaaa
     5521 caatcttaaa tccttactta catttggaat cttgtaattt tttataatta aatcttttat
     5581 atcttcacct aaaatttcaa tatctgaaaa atcttcttgt atatatcctc tcttaatagc
     5641 acctctaatt gtagcaactc tatttggaag taaacttata atcttacatg caacaacatc
     5701 tatagcatat ggattttgtg atgctaataa aactcctatt tctcgagtag taccagctga
     5761 aggtccatca ccttccatac ctactatacc atccataatt gtcaatgttg gttttatata
     5821 atcacaaata tctaaaagta cattttcgca aaacaactct tctgtaggaa atctataatg
     5881 tatctctgct tttttcaatc cagctatgca tccatataag ttttttgtac caccagtaaa
     5941 agttgccatt atatgagtct ttaatttaca aagatttatt atataatctg aatctactat
     6001 tggtttaata atatccatat attttagaac atgtgctttt tcactatgta tcttaacatc
     6061 tgaaacatca taatttaact taatgcccag ttcatttgct acttcttcaa ttccagatgc
     6121 cttataaatg ctcttaagag aactttgatt atatggacca ccagggctat ctgctattgt
     6181 aatatcacaa tttaactcta aaagtttttc acatacaact tttacaacca taggatgtgt
     6241 agttgtagct tcttcaggct tttttttcat taataaattt ggttttataa gtactttgct
     6301 tcctttttta acaaacttat ctataccacc aatatcctct aaattttttt caatagataa
     6361 ctttacttct tcataattgt aagttttaca actcctaaca gataccgact tcatattatc
     6421 tccatccttc tatcacaaca gtttatttat ataatttaaa aaaggagaaa ccaaagtttc
     6481 tccttttaat ctatgtttta attgttttaa ttattatttt ccaaaatgtc tgtttaataa
     6541 tcctaagaat gctttaccat gtctagcttc gtctttacac atttcatgta ctgtatcatg
     6601 tatagcatct aatcctaatt ctttagctct cttagctaat tttaattttc catcagttgc
     6661 accatactca gcatcaactc taactcttaa gttttctttt gtatctgcaa ctacaacttc
     6721 tccaagaagt tctgcaaatt tagcagcatg ttcagcctct tcaaaagcta ttctcttata
     6781 cgcttcagct acttctggat aaccttctct atcagcttgt ctactcattg ctaaatacat
     6841 tcctacttct gtacactcac cagtaaagtt agctcttaat ccttcgatta tttctgcatc
     6901 tacaccttga gctactccta ttctatgttc atcagcccaa accatttcgc ctttcatttc
     6961 ttcaaattta tcagctccaa ctttacatac tggacattgt gctggtgcag catctccttc
     7021 atgtatatat ccacatactg tacaaacaaa ttttttcata attaatttcc tcccaatttt
     7081 atataattaa atttttattt ttaagtttaa taacttgcta caagaatata tataccccag
     7141 actttatagc ttaaacacta aaaataaaat ttatttccta attaatttta tcactttgaa
     7201 acaccagtat caaccaagtt ttgatgtcaa atttctacat aatacataat ttatttgatt
     7261 aattaggaaa tagttgtcat attaaatact ttctaatgaa gactcctctg aatctttaag
     7321 tttcactttt ttgctcttta aaaaagataa tttagtttct gcagttatga ttgcaacaaa
     7381 tatcaagata ctaccaataa ccaattttgc tgtaataagc tcctttaaaa ttattataga
     7441 aaatattgta ccaaatacag cctctgtgga taatattata gctgttctag ttccatcaac
     7501 tcttttttga catatagtct gaaataaaaa tccaattgta gtactaaata cagctagata
     7561 catagttccc atgtaaccag ataattcagc ttccatcttt aattgacctg caaatgtttg
     7621 aactacaaca gacattaaaa acgctactgt aaattgaata gctgtcaatg ccattgggtt
     7681 gttatctttt gcaaactcac ttgtaaagaa tatatgaaat gcaaatccga atgaacatat
     7741 aagtgttaga aaatctccaa aattaataga gaaatcagct tctaaagaaa gtatcccaat
     7801 tcctataaga gctaccaaac tacttattat tcctatttta tcaagttttc ttctataaag
     7861 tataaatcct atgaacggaa ctattacaac attagctgct gttataaacg catttttaga
     7921 tggtgtagtg tacataagtc caatagtttg tactgtgaat gctagaaata aaaatattcc
     7981 taaaatccct cctgctttta ataatttttt acccatattg gcacgaatct gtttaaaaaa
     8041 cagtaaattc ataattatag ctgccaagaa aaatcttaaa gttatgattt gtagaggtgt
     8101 aagcccaccg tctaatgcaa attgggttgc tacaaagcca cttccccata taattgctat
     8161 aaatattagt cctatttctc caaagtattt tttcaataaa gccccctcct ttttattttt
     8221 ttgcaaaatt ataaaccccc taaacagcaa gagagttttt ctataagcat tactataaat
     8281 taatatagta aattttttca gtattttaaa tctaaattta catacatttt gagctcaaat
     8341 ttatatccac tatttaacag taatatatta atcatttttt gtaaacacct ttttaataaa
     8401 tttttatat
//
LOCUS       DAGSHT010000145         8407 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGSHT010000145 DAGSHT010000000
VERSION     DAGSHT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.150"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            146..730
                     /locus_tag="MJB72_002785"
     CDS             146..730
                     /locus_tag="MJB72_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBY8263861.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(834..1613)
                     /locus_tag="MJB72_002786"
     CDS             complement(834..1613)
                     /locus_tag="MJB72_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY8263862.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1671..2927)
                     /locus_tag="MJB72_002787"
     CDS             complement(1671..2927)
                     /locus_tag="MJB72_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBY8263863.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3185..4084
                     /locus_tag="MJB72_002788"
     CDS             3185..4084
                     /locus_tag="MJB72_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBY8263864.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            4108..5556
                     /gene="ddlR"
                     /locus_tag="MJB72_002789"
     CDS             4108..5556
                     /gene="ddlR"
                     /locus_tag="MJB72_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBY8263865.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5615..6421
                     /locus_tag="MJB72_002790"
     CDS             5615..6421
                     /locus_tag="MJB72_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY8263866.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6447..8366
                     /locus_tag="MJB72_002791"
     CDS             6447..8366
                     /locus_tag="MJB72_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY8263867.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
BASE COUNT         3183 a         1144 c         1198 g         2882 t
ORIGIN      
        1 atttactgtt attatgaata tggttgaaat tatttaaata aatactgttg acttagtttc
       61 atatatttac cattaaactt ttatcataaa tatcatatca taataatata tttaataagt
      121 ctaataaact atacgaggtg taaatatgtt aatgaatacc cattttatta tggcaaaatc
      181 aatacttgat aatatagatg aaaataaaac attctttata agcgaaaaaa attttatata
      241 tggcaatata aagcctgatg ctttttctaa atataaatta aaaaaacatt atttagatga
      301 gtcatttaat atgattgttt ctaaaataaa ctatctatgc agtcttaata tagatagtct
      361 ttctaaatac ttttcagtaa gtagacttag tcaggaacta ggagtcatat gtcatttttt
      421 atgtgatttt ttctgtgtag ctcacagtga acgttgggaa tttaagcata gcttaaataa
      481 acatgttact tatgaaagag aacttggagc atttgcaaaa gatgttgatt tatctaaaat
      541 taaatgtggt actataaata gtagttttga gaagtttttt actaaacttt atagtgatta
      601 caaaaagaaa actgattata tgaatgattt aaaattttca gcttatactt gtaatagtgt
      661 cataaattat atacttgact ctattttatc taatacaata aaatcatatc atatagcaaa
      721 ttgcggttaa cttaagaatt tttaaactta tataaactta taattgaata taattaaaat
      781 gactgctcta tagaatataa ttttatactt ctctaggaca gtcatttttt atactaacta
      841 tttatctcca ttataacatc tatggcactt tttacactat ctactacata atttgcactt
      901 tgctttacat cttcccttgc cttagacatt gcaaaactat atttaaatcc ttctaacatg
      961 caaatatcat ttggagaatc cccaatagta gcaacttcat ctggacttac atttaacgtt
     1021 tttattacat gctctatact tcctcttttt gacacacttt taggaactat atttaaagta
     1081 tatgttccag aaaaatatat ttctaagact tcactaaact cactttttag ataaagttct
     1141 attctggata actcttccaa attttcagaa catacgccta tatttcctat aagattatca
     1201 aaactacttc ttttttcaaa atctgggtca actataaagt cactagaata atgtctaaaa
     1261 gcagtggtat cactacccac aattacatca cccttatatt taaaataaag ttgtgcatca
     1321 cttgactcaa atctatcaat gacttttttt actaaattat catctatcgt tcttttaaaa
     1381 attacattat aatctttatc atatataaat gaaccatttt cacatacata gtatccattc
     1441 atatctaaac tatctttgat aatattatgt gcttgtttaa aaattctacc tgtagcaaca
     1501 ttaaactgta ctcccttttt ttctatttct tcaattgcta ctatatcttc ttttgttatg
     1561 gttccatttt catatagagt tccatctaaa tcacaaaata tatgttttat cataaattaa
     1621 aagcctccaa aaacaatttt atattttact aatactttaa tcaaaataac ttaaagcttt
     1681 tctaattgta ttctcaattc attaagcaaa tctctagtag ctcttaatct tatctttctt
     1741 ctatccccat taaatatatt tttctttact attgtttttc catttatata tattgcagta
     1801 taaaccagtc ccacaggctt ctctttggtt cctccaccag gtccagctac tccagttgta
     1861 gatataccta tattagcttt aagtccttta gcaatgccct cagccatttc tatagctgtt
     1921 tgctcactta ctgctccaaa ctcttccaaa gtttctcttt ttactcctaa tcttttcatt
     1981 ttagaatcat tagaatatgt tacacaacct tcaagaaata cagaggatat acctggatag
     2041 tttataagag atgaagaaac caaacctcca gtacaagatt cagccactgc tattgttaga
     2101 tttttttcta caagtagctt agaaacagca tcttctaaag cagtctcccc ttcagatatg
     2161 gaaatatctc cttcagtata tacatattta ccaactctac tttttatttt ttcttctact
     2221 ggttttatta atttaaaagc ttctctttca ttttcagcct ttgcagttat tcttattgta
     2281 acttctaatt cttttgcata taaagcaact gttggattag tctgctcatt aattatatcg
     2341 agaatttcta attctagatt tgactcacct attccatata atcttaaagt cttagatact
     2401 agcatttcat ttgtgaattg ttgtaagtaa ggtttgacac tctcattaaa catagctttc
     2461 atttctcttg gaggtccagg caatactatt ataaatttac catctttttt taaaattgcc
     2521 cctggagctg taccattatt attttttagt actatagcat cttttggaaa gtacacttgt
     2581 ttcatattat tttcggccat ttttttccct gttttaacaa agaaagattc caaaacctct
     2641 agtgaaggtt tatgtagttc taatttctga ccaaagtatt ctgcagcagt ttcttttgtc
     2701 atatcatcac cagttggccc aagaccacct gtagtaatta caaaatcact tctctttaag
     2761 ctttcatcaa aacattctaa aagtctttgt ttgttatctc caacagtact ctgatggtat
     2821 acttctattc ccaatgatgc aagttcttta gctaaaaatt gagaatttgt atttactata
     2881 tctcccaata atatttctgt tcctacactt attatttcag ctttcaaaat ttatcactcc
     2941 ttgatttata gccattaaga ctagtcttaa gttcataaca ataattttag caaaaataat
     3001 aacataaata aagttaaaaa atcatttaaa catatcttta aactagatta ttattctaaa
     3061 accacacata atataaactt cgtatatagt ggtactttca tcctcaactg ttaattcata
     3121 taattaagta tgtaaataaa aagtttactt taaaataata aaaattaaaa taggagtgta
     3181 taatatgaaa atagcagtta taatgggtgg aatatcatca gagagagaag tttctttaaa
     3241 ttcaggaaaa gagatatata ataaccttga taaaaataaa tatgaagttg taaaggtaat
     3301 aatagatgat aaaaaagata tatttacaaa gataccagaa gatatagatt ttgcaatact
     3361 tgctttacat ggaaaatttg gtgaagatgg atgtatacaa tcaatacttg agactatgga
     3421 tataccatac tctggatgtg gacctctttg cagtgggatg tgtatggata aaaatataac
     3481 taaaaaaatg cttagagatt caaacctacc tactgctcca tgggttttag taaaatcagt
     3541 tgatgaaata gactatgatg aaatagataa tataggttat ccagtattta taaaaccaaa
     3601 ctcaggaggt tcaagtgtag ccacattctt tattcattct aaggatgaag ttgaagaagc
     3661 agttagaaaa ggtctggaag ttgatgaatt tgtaatgatt gaaaaatata taccaggtgg
     3721 agaatatact tcattcatat taaatggaga agtgttccct acaatatcta taaaatctga
     3781 ttcaggattc tttgattatg aagctaaata ctcagttgaa aaaggtgcaa aggaagaagt
     3841 tgtttactta gatgaagaac ttcaaaaaag agtaaacgaa atttctgaga cttgttggaa
     3901 aatttttaat tgcaaagcat atgtaagagt tgatatgatt ataagtgaag gtattccata
     3961 tgttcttgaa ctaaatactc ttcctggaat gactcaaact agtttaattc ctagaagtgc
     4021 tgctgctaga ggtattaagt acagtgaact attagataaa cttatagaat actctttaaa
     4081 ttagatgaaa taatttgagg tggtaatttg atattttcaa atctaaaatt aaatgataat
     4141 gaaccaattt atatacaact aaaaaattat ataagtgata tgatatcaaa aggactaatt
     4201 ccagacaata gtaagctccc ttctacaagg gagcttagtc agcttttaca agttagccga
     4261 aattctgtag ttttaactta tgaagaatta aaatcagaag gattaatcta ctctatttca
     4321 ggaaaaggta catttgttaa atcaaagaat aagtcttcaa atactacatg gtcattaaac
     4381 tgggattgtt tagagaatac atactctaaa aaagccaatg aattggacat tataaaaagt
     4441 gaaattcctt ggagctctga cctgatttcc tttaaaagta tttctccaga tggagattta
     4501 ttcgacatgg aagaattaaa aaaatccttt ttaaatagaa tttcactaga aggacataaa
     4561 ttactcaact atggatatgc acaaggttat aagccactta ttgactattt actagaatat
     4621 atgacaaaca aaggtgctga tataagtaat aaagatatac taataacaaa tggattcaca
     4681 gaaggctttg atattattat gtcatctttt acccaagaaa aagactacat aatatgtgaa
     4741 aatcctactc ataatactgc tataaaaatt atgaaatctc ataacctcaa tatcttaggt
     4801 gtagatataa atgaagatgg tttggacttc aatatgctag aagacaattt agttaagtat
     4861 aaaaataaaa taaagtttgc atacattaca ccctcttatc acaatcctac aggaattgta
     4921 atgactcctg aaaataggta caaattctat aatcttatga aaaaatacaa tatagcaatt
     4981 atagaagatg gatttaatga agaattactc tacaatagtt ctcatatatt tcctatctgt
     5041 tccctagata atatgaataa tggggtcgta tatattggta gtttttcaaa aattttattt
     5101 cctggaatga gaattggatg gatatttgca gataaaaaag taatcaataa gctagaaagt
     5161 gtaaaaaggt gcagaaacat acatgtatcc tttttagacc aaggaatttt atttgattat
     5221 ttaagtaatg gaggttttga aaaatacatt aaaaaaatac gtaaattcta tggagacaaa
     5281 tttaactttg catataaatg tataaaaaaa tatataaaaa cagaatatat cttgggtgat
     5341 ggtggtttgc atatatttat aaaactaaaa aatatagata ctagagtttt attagaaaaa
     5401 tgttatgaaa aaaatgttat atttatgcct ggagacttat tttttacaga taactctggt
     5461 tttgatactt taaggctcgg attttctagg ttgtcttttg aagacattga aattggaata
     5521 aaaataattg gagaaactat aaatgaaatg ttctaggtta actaaatatt gttagcctag
     5581 tttattttta tgaggtataa tatatatagt ttatatgaat ctaatactgg gggataatat
     5641 gagtaaaaag tcaaaacgaa gaaaaattaa taaattaaaa ttaacgattg taataactgt
     5701 attatttctt gctttattat ctatatatga gatactgttc tctaatccca acaaaaaagg
     5761 taatataaag agtcaagaaa ctatgcaaaa taaaaataat acaacagatg attctaaaaa
     5821 taatcaaagc tctaccaaaa agcaggaaga taataatatc aaacctactt tttcatattc
     5881 ttcaatacca gataatataa aaaataaaat gattggtaaa tctatgccta ctgatgaacc
     5941 tataagcttt gatagtctat cttatttaaa acttacatat tatggatttg atgaaaaaac
     6001 tcatcaagga gaaatgatag taaacagtga actagctcca gaagtagtgg atatttttaa
     6061 ggaattatac gaaaaaaaat atccaattga aaaaattaag ttaatagatg actatgatgc
     6121 tgtagatgaa aaatctatgt ctgataataa tacctcatca ttttgttata gaactatagc
     6181 tggtacaaat gtagtttcaa atcatggtaa aggacgtgct atagatataa acccattaca
     6241 gaatcctcaa gtttctggaa atgatgttac acctaaagta agtacagttt atgctgatag
     6301 gtctagcaca aaatttggaa tgataaaaaa aggtgatgat tgttataatg cttttgtaag
     6361 cagaggttgg agttggggtg gctattggaa aaacccagat tatcaacatt ttgaaaaata
     6421 atagattaga aataaaggtg aattatatga atttaatgac attggaaaat ataagtaaaa
     6481 gctattctga aaaaaaatta ctagaaaata tatctcttgg aataaatgaa ggggaaaaaa
     6541 taggatttat tggtgtaaat ggtactggta aatctacctt actcaaaatt attgcaggag
     6601 ttgaagaaag tgagactggt aatataacaa aaactaatgg tattaggata gaatatctac
     6661 ctcaaaatcc aacttacaat gaaaattcaa ctgtacttga acaagtattt aatggaactt
     6721 ctgaagaatt aaagctactt ggtgaatatc aagaaatcct agaaaaactt aattcaaact
     6781 ttacagagga attaaataaa aaattattat cagtacacga aaaaatagat gccttaaatc
     6841 tttgggattt agaaagtgaa gctaagtcag tacttacaaa gcttggtata aatgacttta
     6901 atcaaaaaat agcagaactt tctggtgggc aaagaaagag agtctctctg gcatcagccc
     6961 ttattacacc ttgtgagttt ttgatactag atgagcctac aaaccatcta gacaatgata
     7021 ctatagattg gcttgaggaa tatcttaatt ccagaaaagg ttcattgctt atgataactc
     7081 atgatagata ctttttagac cgtgttacaa atagaattat tgaactggac aaaggtagat
     7141 tattcagtta tgatgggaat tattcaatat ttttggaaaa gaaaatggaa agactttcgc
     7201 tagaagcaag tattgaagat aaaagacaaa atttaataag aacagagctt gcgtgggtaa
     7261 gacgaggagc tcaggctcgt agcactaaac aaaaagctcg tttacaaaga tttgatgaac
     7321 ttataaatag agattctttt aaacctgatg aaaatctcga tatatctgtt gcatcaagta
     7381 gacttggaaa taaaataata gaaatacaca atatatctaa atcatttgaa gaaaatactg
     7441 ttatagataa tttagaatac actttaactc gtacagatag aattggtata ataggcaaaa
     7501 atggtatggg taaatctacc cttataaata tacttaatgg agaaatagag cctgatagtg
     7561 gatatatttc aattggagaa acagttaaaa taggttgttt ttctcaagac gactctcata
     7621 tgaatattga tatgagggct atagactatg taaaagaagc tagtgactat atagaaactt
     7681 cagatggtac taggattaca gcttctcaaa tgtgtgaaag attccttttt aatggtacta
     7741 tgcaatatac tttgataggt aaattatctg gtggtgaaag aagaagacta catcttctta
     7801 aaatccttat gacagctcct aatgtgcttc ttttagatga acctacaaat gatttagata
     7861 ttgaaacgct taaaatatta gaagaatacc tagacgaatt taaaggtgta gtagttacag
     7921 tatcacatga tagatatttc ttagatagaa tttgtaataa aatatttgca tatgaaggta
     7981 atggaaaaat tcatatctat acaggtaatt acagtgatta tcttatttat agagagattc
     8041 aaggaattga atttgaagaa tctaaaaggg aatcagttaa aaaagatgac tctactaccc
     8101 caaaaaaaga aaaacctaaa aatgataaaa agcttaagtt ctcatataat gaacaaaggg
     8161 agtttgaaaa tattgactct gatatagaaa aactcgaatc taaaattgca acacttgatg
     8221 aaagtactgc aaaattttca actgatttta ctaaactcca agaaatttta gatgagaaat
     8281 ctaaactaga aaaagaactt gaatacaaat atgaaagatg ggaatattta aataatctag
     8341 ctgatgaaat agctaataat aagtaatatt aaaataatct taagtttaat aagttttgct
     8401 ttaaatc
//
LOCUS       DAGSHT010000146         8288 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGSHT010000146 DAGSHT010000000
VERSION     DAGSHT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.151"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            214..369
                     /locus_tag="MJB72_002792"
     CDS             214..369
                     /locus_tag="MJB72_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263868.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            499..1512
                     /gene="aroF"
                     /locus_tag="MJB72_002793"
     CDS             499..1512
                     /gene="aroF"
                     /locus_tag="MJB72_002793"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBY8263869.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1534..2598
                     /locus_tag="MJB72_002794"
     CDS             1534..2598
                     /locus_tag="MJB72_002794"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBY8263870.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFDKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2598..3911
                     /gene="aroA"
                     /locus_tag="MJB72_002795"
     CDS             2598..3911
                     /gene="aroA"
                     /locus_tag="MJB72_002795"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBY8263871.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGIKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3889..4968
                     /gene="aroC"
                     /locus_tag="MJB72_002796"
     CDS             3889..4968
                     /gene="aroC"
                     /locus_tag="MJB72_002796"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBY8263872.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4971..6167
                     /gene="pheA"
                     /locus_tag="MJB72_002797"
     CDS             4971..6167
                     /gene="pheA"
                     /locus_tag="MJB72_002797"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBY8263873.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6214..7023
                     /gene="aroE"
                     /locus_tag="MJB72_002798"
     CDS             6214..7023
                     /gene="aroE"
                     /locus_tag="MJB72_002798"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBY8263874.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            7035..7568
                     /locus_tag="MJB72_002799"
     CDS             7035..7568
                     /locus_tag="MJB72_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBY8263875.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7589..>8288
                     /locus_tag="MJB72_002800"
     CDS             7589..>8288
                     /locus_tag="MJB72_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBY8263876.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGN"
BASE COUNT         3391 a          825 c         1502 g         2570 t
ORIGIN      
        1 tggaattata atatttaaaa taaaaaaatt attgtaaatt acagatgatg tttaattgat
       61 tttataaaaa tacatcaatt gagaagctga tttaagtaaa taaaaaaata ttttaaattg
      121 aaaatattta tagaaaatat tgacaattat attttctttg ttataattat taaacaagaa
      181 gaataataaa ttataaaaat ggaggataat actatgaaag gaatattaaa agaactaaca
      241 gcaaaacttt atagcttttt ttactttatg ttctggggct tttattttag cgctggctat
      301 ttcttttaca aaaatatatg tacttccttt ctggtgaggc cttattttag tgattttgag
      361 cataagtaaa ttttataaaa atggttattt tatatattta tataagattt agtgcttgat
      421 agattatttt agagaactca ttagtgagtt ctctttttta ttttaaatat aaaattataa
      481 tattgggagg tagtaaaaat gatagtagta ttaaaaatgg gagcagacaa aaatgaagtt
      541 aaaaaattaa ttgaggctat tgggagagaa ggagtagaag ttaatcccat agatggaaca
      601 gaattaactg ttttaggttt agttggagat actagtaaaa tagatgccaa aagaatagaa
      661 gcaaataagg tagttgaaaa agttatgcat gtggtagagc cttttaaaaa ggccaataga
      721 aaatttcatc cagaaccttc aatcataaat gttaatggaa tggaaattgg ttcaaagaaa
      781 attgctatga tagctgggcc ttgttctgtt gaaacagaag accaaatagt atctatagct
      841 aaagatgtga aaaaatcagg tgcaggattt ttgagaggtg gagcattcaa accaagaaca
      901 tcaccatacg ctttccaagg attgaaatat gatggattgg atttattaaa aaaagcaaaa
      961 gaaaaaacag gacttcctat agttacagaa ataatgtcaa cacaagatat agatatattt
     1021 gaagaaaatg tagatgtaat tcaagttgga gctagaaaca tgcaaaactt tgatttatta
     1081 aaagaattag gtaaaacaaa taaaacaata cttttaaaaa gaggattatc tgctacaatt
     1141 gaagagtggc taatgtcagc agagtatata atggctggag gaaatgaaaa tgtagttctt
     1201 tgtgaaagag gtataagaac ttttgaaaca tatactagaa atactttgga tttaagtgca
     1261 attttagctg tgaagaaact tagtcattta ccagttatag ttgacccaag tcatgcagca
     1321 ggtaaatcat ggatggttga ctctttatct aaggcagcaa ttgcagttgg agcagatggt
     1381 ctaataatag aagttcataa tgacccagca catgcacttt gtgatggaaa acaatcgatt
     1441 aagccaaatg aatatgatga attgataagt gaattaaaga ctatcgcatc agcagttggt
     1501 agagaaatat aatctataaa aggatggtta aacgtggaag aaaaactatt agtaaattta
     1561 aaagaaaata gctacaatat atttattaaa aaaaatatac taaagaatat aggagaagaa
     1621 ataaaaaaaa tatattttgg aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa
     1681 ttatacttgg ataaagtaaa aaataattta agtgaatctg gatttgaaat taaatcttta
     1741 atattaaaat ctggtgaaga aacaaaatca tttaatactc ttcctaaaat atacgatagt
     1801 ttacttgact ttaagttaac tagaaaagat ttgattatta ctttaggagg aggggtaata
     1861 ggtgatttag gtggatttgc agcagcaact tttttaagag gtataagttt tattcaaatt
     1921 ccaacatctt tattagcaca agttgatagt tctgtaggtg gaaaagttgg agttgacctt
     1981 gagagaggca aaaatttagt gggaagcttt taccaaccaa aggctgtgtt tatcgaccca
     2041 aatgtattat tatcacttcc agaaaaattc tatagagatg gaatggcaga agttataaaa
     2101 tatggatgta taaaagacaa aacttttttc gataaacttt taagtttaaa aaatagagaa
     2161 gaagtaatgc aacatataga atacattata cataaatgtt gctttattaa aaaaactgta
     2221 gttgaagctg atgaaaaaga ctttggagat agaatgcttc taaactttgg ccatacatta
     2281 ggtcatgcta ttgagaaata ctacaatttt acgggttata cccatggaga agctgttgcc
     2341 ataggaatgt ataagataac tttagaaagt gaaaagatag gaattacaaa aaaagggaca
     2401 tcagaactta taaaatcaat tttaatgaat tataatttgc cttatgagat tgaattaaga
     2461 gataataaat ctatagtaga tgctatatct cttgataaaa aaaatctagg tgatacatta
     2521 aaaataatac ttttaaaaga tattggagag agtattatat ataatactac ttctaaattt
     2581 tttcaggagg taagataatg ggaagtctaa aaatatatcc aagtaaatta tctggagatg
     2641 taaaaatacc accatcaaaa agtatggcac atagggcagt tatttgttca tctctaagca
     2701 atggaaaaag tagaatttct aatatagatt tttctgatga tataatagct actataagag
     2761 caatgacatc attaggagct attatagaaa agaaagaaga tattttagaa atttcgggga
     2821 ttttttctaa agaaggtatt ttaaatagag agaatcagtt gaatcaacct aaattgacaa
     2881 tagattgtaa tgaatcaggc tctacattga gatttttagt acctatatct ttagcatttg
     2941 atggtataaa aaggtttata ggtagaggta atcttggaaa aagaccactt gatacttatt
     3001 atgagatatt tgacagacag aatataaaat atagttacaa agaaaatcag ttagatttga
     3061 ttatatcagg taagcttaaa ccagatgaat ttagagtaaa aggaaatata agttctcagt
     3121 ttatcacagg tctactgttt atattgccta cgctagagag tgattcaaag ataataatta
     3181 ctactgagct agaatctaaa ggatatttag atttaactct ttcaacgata aaagattttg
     3241 gggtagaaat tataaacaat aactataaag aatttattat aaaaggaaat caaacgtata
     3301 aagcgagaga ttataaagta gaaggagatt attctcaagg agcattttat ttatcagcag
     3361 atgctatagg agaagatatt agcatattag accttaaaga agattctctt caaggggata
     3421 gtgaagtagt tgaaatttta tctaggatgg gaatggaaat attaagagaa ggcaacaaaa
     3481 taaaaggaat aacaaatggt ttaaatggta ctttgataga tgcatctcaa tgcccagata
     3541 taatacctgt attatctgta gtagcatcct taagtatagg gaggactaca ataattaatg
     3601 ctggtaggct tagaattaaa gaatgtgata gacttcatgc aataaatgtt gagttaagta
     3661 aacttggagc aaatatagag gagaaagagg atagtttgat aatcgaagga gtaagcaagt
     3721 taaatggtgg agtagaggtg tggagtcaca aagaccatag aattgctatg acacttgcaa
     3781 ttgcttcttg tagatgtgat aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat
     3841 cttatccaca tttttttaaa gattttaaga tgttaggagg tcgaattgat gagtggaata
     3901 tggggaaata atttaaaagt atcaattttt ggagaatctc atggaaatgc tataggtata
     3961 aatatagatg gtcttccttc ggggattgaa cttgaccttg ataatattga taaagaaatg
     4021 aagagaagag cacctggaaa aaattctata tctacttcaa gaaatgaaag tgatatacca
     4081 gaaattctaa gtggatattt taatggaaga actacaggaa cgccattatg tgcaatcata
     4141 agaaatagtg atactcgttc aaaagattat ggagagctta aaaatttaat gagaccagga
     4201 catgcagatt tcacaggtaa tgtgagatat tctggattta atgactatag agggggaggt
     4261 catttttcag gtaggataac agctccatta gtattttgtg gggctatatg taaacaaatt
     4321 ttatcacaaa aaggaataga gataggagca cacataaaaa aaattaaaaa tatagaagat
     4381 atgagttttg attatgtaaa tatttcaaaa caacagttat caaatttgca aactttggaa
     4441 ctacctttac ttgatttatc aaaggaagaa gccatgaaaa atacgattat agatgcaaaa
     4501 aatcaaggag attcagttgg tggtataatt gagtgtgcag tagtaggtat taatgtaggg
     4561 cttggaaatc cattttttga ctctgttgaa tctactttat cacatttatt gttttctgtt
     4621 cctgctgtaa aaggtgtaga gtttggttta ggttttgaac ttgcagatat gtatgggtct
     4681 caaagtaatg atgaaatgta ttatgaagga aatcaagtta agagcaaaac aaataacaat
     4741 ggaggaatta taggagggat tactactgga atgccaataa ttttcaaagt ggcaattaag
     4801 ccaactccat caattagcag acaacaaaac acagttaata taaaagataa aaaggacgat
     4861 attttatata ttaaaggtag acatgaccct tgtatagttc aaagggctat acctgtaata
     4921 gaagcagtaa cagccattgg aatttttgat ttgatgaaag gacgataata atggaggatt
     4981 taacgagata tagaaatgag attgacaata tagataaaga gcttattcaa ttatttgaaa
     5041 agagaatgaa tacagttctt gaaattgcca gacataaaat gaaaaataac actactatac
     5101 ttcaaaaaga tagagaagaa aaagtactaa gtagagctgt agataattta gataataaag
     5161 catattcaca agaaacggta cagtttttta attctataat ggaaattagt agaggattac
     5221 aaaaaagatt gatagataat gatggtgagc agaaaatcga gtttagttta gattcatcaa
     5281 ataagaaatt tgatttgtca aatgtaaata gatataagtc tttaaaagat gaattaaata
     5341 aaaaaaatat cctagtaggg tttccaggaa aatcaggttc atttacagaa gaagcactaa
     5401 ataaattctt caacaaaaaa atatcaaaaa aacaatttaa agaatttgaa gatgttttca
     5461 tagcacttaa aaataaagaa ataaactatg gaataatccc catagaaaat tcatctacag
     5521 gagctatctc tgaaacttat gaccttttga gaaagtacgg tttttatata gttggagaag
     5581 aatgtataaa aatagaccaa aatttaatag ggataaaaga cacaaagctt gaagatataa
     5641 aagaaatata ttctcatcca caaggtatag gacaatgtag tgaattttta aaacaaaata
     5701 gtacatggaa attgatacca tttaataaca cagcaacaag tgcagaactt gtaaggaaac
     5761 ttcaagataa aacaaaagca gcaattgcaa gcaaaaaagc tgcaagtatt tatggattag
     5821 aaataataag tccttgtata aatgatattg caaacaacta tacaagattt gtagtaatca
     5881 gtaatcaaat acacattgaa gaagaatcaa acaaaatgag tgtagtattt tctgttgaac
     5941 atgaagcagg aaaactttat aaagtacttg gatactttgc taaaaataat ataaatatga
     6001 caaaaataga atcaagacct atgaaaaatg cctcatggag atatttcttt tatatagatt
     6061 ttgagtgttc tatatacaat tctaaagtgt acgatttatt agaattgata gaacataata
     6121 cagcatattt taaatttatg ggagtatata aaaataaaac tgaatagata agataaattt
     6181 ttatttaatt tataattatt aggaggtgta agtatgaatt ttttcggtct agtaggtgaa
     6241 aaactctctc acagtgtttc acctcaaatc cacaagagag tattcgagat attgaatatt
     6301 gaaagtgctt ataaaaactt tgaaatttca aaagaagata tatctaagct agacggtgct
     6361 ataaaattat taggaataca aggagtaaat gttactgtac catataagga gcgtattatg
     6421 aaatatcttg attttatatc accagaggca aaacgtatag gagcagttaa tacaatttta
     6481 ctcagagaaa atatgttata tggttacaat acagattact ttggtcttga tagtatgttt
     6541 aaaatggcta atattgatgt tcaaggaaaa gtagcagtta tattaggaac tggaggtgct
     6601 tctaaagctg ctcttacata ttttattgat tctggtatag aaaagcttta tgtttcaact
     6661 agaaaaaagg atgataaaaa attattaaat agtaaagcta tattaataga ttatgaagaa
     6721 cttaaacata taaaaggaga tataatatta aatgctacac cagtaggtat gtaccctaat
     6781 gtaggaattt caccagtgtc aaaatctatt atacaaaatt ttgatatatt aatagattta
     6841 atttacaatc ctggagaaac agagttttta agaattggta atagtatggg taaaaaaact
     6901 tgtgatggat tatatatgct ggtggggcaa gcaataaaat ctcaggaaat ttggcaagat
     6961 actaaaatag ataatagtat tttagatgtt atatataatg aactaaaatt agaatttttg
     7021 taagggagat tgttatgata aataaaacaa aagaaaaatt gatattaata ggtatgccag
     7081 gtagtggaaa aactacaatt ggtaaacttt tggccaaaga atataattgt agtttctgtg
     7141 atatggatga ttatataatt caaatatctc aaaaaagtat agcagagttg ttttcagaag
     7201 gagaagatat ctttaggaac tatgagactc aagcatgtag agaactttct ataagtgata
     7261 aaacagtgat atctacaggt ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa
     7321 aagaaacagg tataataatt tttattgata gacctataca aaagattttg gaagatataa
     7381 atattaattc aagaccatta ttaaaaaacg gaaaagatag attgtataat ctttacaatg
     7441 agagaataaa tctttataaa aaattttcag atatagagat tttaaatgat aaatcattaa
     7501 ataatgctgt atataatatt acaaatgcag tcagtgaaaa ttttaaattt gactttaaag
     7561 aaaaataatc tagggggaag taaaaataat gaatattgtt attgtaggac taggagttat
     7621 aggaggctct tttgctaaag cattaaaaaa agctggatat gaaaatgtat ttggagtaga
     7681 tgtagattta gaaactttaa aaaaagcaga aaaagcaaaa atcataaaaa aaggatgtac
     7741 tacaggtaaa gagttattta aaaaagctga tttaattata ctatctatat atcctagatt
     7801 agttgtagat tttctaaata ataataaaaa cttttttaaa aaagggacta taattactga
     7861 tactacagga ataaaagaaa cacttatcaa tgatgtgttg caaattattc cagatgatat
     7921 agattttata tttggacatc caatggcagg tagggaaaaa aagggtattg attttgcaag
     7981 tgaacaagtt tttaatggcg caaattatat aattactcca actggcagaa acaacataaa
     8041 aaatttagag ttagttgaaa atttaatttt agagattgga tttaaaagag taaaaaagtt
     8101 aacttcacaa aaacatgatg agattatagc ttttacttct cagttacctc atgttatggc
     8161 agttgctctt ataaatagtg atgaagaagg tagagatacg ggtaaattta ttggagatag
     8221 ttatagagat ttaactagaa ttgcaaatat gaatgaagac ttatggagtg aacttttttt
     8281 aggaaata
//
LOCUS       DAGSHT010000147         8287 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGSHT010000147 DAGSHT010000000
VERSION     DAGSHT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.152"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(66..1097)
                     /locus_tag="MJB72_002801"
     CDS             complement(66..1097)
                     /locus_tag="MJB72_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBY8263877.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            1285..2175
                     /locus_tag="MJB72_002802"
     CDS             1285..2175
                     /locus_tag="MJB72_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY8263878.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            complement(2227..3153)
                     /locus_tag="MJB72_002803"
     CDS             complement(2227..3153)
                     /locus_tag="MJB72_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBY8263879.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKNISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(3368..3952)
                     /locus_tag="MJB72_002804"
     CDS             complement(3368..3952)
                     /locus_tag="MJB72_002804"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBY8263880.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            complement(4037..4939)
                     /locus_tag="MJB72_002805"
     CDS             complement(4037..4939)
                     /locus_tag="MJB72_002805"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBY8263881.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            complement(4932..5624)
                     /locus_tag="MJB72_002806"
     CDS             complement(4932..5624)
                     /locus_tag="MJB72_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBY8263882.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            complement(5663..6583)
                     /gene="pyrB"
                     /locus_tag="MJB72_002807"
     CDS             complement(5663..6583)
                     /gene="pyrB"
                     /locus_tag="MJB72_002807"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBY8263883.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            complement(6879..7901)
                     /locus_tag="MJB72_002808"
     CDS             complement(6879..7901)
                     /locus_tag="MJB72_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY8263884.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
BASE COUNT         2644 a         1506 c          885 g         3252 t
ORIGIN      
        1 ttataaatta ttagtatcat attattttaa ttaatcaaat taatattaca aatactattt
       61 ttatgctata taaatatttt tattaaaact acagctgata caacttgtac tgtacctaca
      121 aataaaccat ataaagaaac ttctttccct tgtttcacta aatcttttac attaaccttt
      181 aatccaattg ctgctaaagc tattatttct agtttgttac ttatttcttt gcacaacaca
      241 gatacttctt gtggtataat gttcattgaa aaaagtgcac aagttataaa gaatccaatt
      301 acataccatg gtatagtcac ttttttcttt tttatctcat ccttttcttc ttcaagaatt
      361 tccctattag attgattttt catatgtcca aatactaata caacaacaac tagaaatatt
      421 actcttacta tcttaaaaat agtagctaaa tcttttacat tttctccaac aattgctcca
      481 cttgctacaa cctgaccaat tgattgcaaa gttccaccta tcattgctga agtttttaca
      541 agttcatggc tatataaaaa ttgagatatt attggaagta aaaacataag aaatatacca
      601 gtaacattta caatagttac tgcaattccc ttttctttat ctgtcgcatt aaccactgga
      661 actgttgctc ctattgcaga agaaccacat actgcatttc cacttgccat tagaaatcta
      721 aagttttctt caaatcctag ttttttacct atataaagtg cagctattat tgttattgtc
      781 atttgcaata ttataaataa aattcctgaa acttttaagt ctattaaggt agatacacta
      841 agtgttgctc ctaatagtac tattgaatat gataacaaat ctgtctctga aaatttataa
      901 cctttttgaa ataccttttg atttaaaaat aggtttccta caaacatacc taaaaatata
      961 gaaattgatg ctgcccctac cttaggaatt aaaatagaaa taaatatact aatataacct
     1021 actaatactg atacaaacag ccctggtaat atttccctta tagtttttaa tgtcaaactg
     1081 ctttttttct ttaacataat atacctacct tataattaat atagttttct tcatagcaat
     1141 agtatataat tgtaataacc ataagtaaaa taaatatata atatcgtaat gattaatgtt
     1201 tttaatggtt attatatata gtataattat ctgtagaaag tatataaaaa atgtaaaatt
     1261 tcattaggta aaggagacaa aaaattgttt gaagaattaa aaacatttgt agctgttgtt
     1321 gaatacaaaa actttactaa agctggggaa tatttaaatc tatcacaacc aagtgtaagt
     1381 aaacacataa aaaatctaga aaattatttt aaagttgttc ttataaacag gtccattaaa
     1441 caaaaaacca tttttataac agaaagtgga caaatcttgt acaaaagagc taaagaaata
     1501 ttaaatcttt taaatattac ttatcatgat gtaagccaag tttcagatgc tataactggt
     1561 cacttaaaga ttggtgcaag tttaactatt ggagagtata ttttaccaaa ttttctagca
     1621 ttattttcta aaaagtatcc tgacatagat gtagaagttt ttataaaaaa tacatcaatc
     1681 gtatcttctc atgttaagga ttatatttta gacattggtc ttattgaagg cacttgctct
     1741 tccccttcat ttattcaaga atattttttt gaagataaga tggttttagc acttccatac
     1801 aagagtcatt tattaaaaga ttttagtttt gataaacttc aaaaccaaaa atggatagtc
     1861 agggaggatg gttctggaac cagagattat ttagatatgt ttttaagtgt taaagaaatt
     1921 attccaaaaa gtatgatggt ttttggaagt aattatgctg tcaaagaatc tgttagaaat
     1981 aatttaggaa ttacaatagt ctctaatcta gtgacaagct tacctgttct aaacaatgag
     2041 ctgtctgtca tagagcttgg aagcagctac aacagacatt tctcatatat atttccaaaa
     2101 gatataactt tatcaaaagc agcaactatt ttcattgaag agttaaaaat atttagtaac
     2161 ttaaatagta tataacatta ataaaattta aaatcaataa ttatcaatat ttcttacaaa
     2221 gaccctttaa aatctacttt caattacttt tatcatataa aagaaagtct gatttatagg
     2281 agtaggtatg ttatattttt ttccaagttt gcagatattc ttagaaaaca tatctacttc
     2341 tgtaagtcta tgagcttcca catcttgaag cattgatgtt cttccttctt ttgaatgttc
     2401 tagtattcta tgtaatgaat gttccacatc atcttctgta agagatatgt tttttgcttg
     2461 tgctatagca acaacttccc tcattgcaga ttttgctaat tctcttaaat gctcagaact
     2521 ctgaaaaact ccataaggtg caccaagtat agctgaagtt tgatttacac caatattaac
     2581 catgtacttc caccacatat ctcgttgaat atcttttgat aaagtatatt ctatatttac
     2641 atcatcaaag acttcggtta ttatattagt ttttctatct tcactattat ctttgttacc
     2701 aaatacaatt atcccattag tagtgtgtat aatattatta ttgattttct ttgcatctat
     2761 attggtaaca tatgaatata ccattttttc aattccaaat ctttctccta ttatctcttc
     2821 gctatcaact ccatttagca gagacattat aactgtattt tttccaacta atccatctaa
     2881 ttcttttata ttttctttta aattattata ttttagacct ataattataa aatcagcctc
     2941 ttgtttatac tcatctttag ttacataatc aaaatcatac ctttttccat ttattataaa
     3001 tccttcttct aaatatcttt tctttctttc attatcacaa agaattttaa aatcatattt
     3061 agagttaaaa aattgttctg caaaagtagc tcctacagca cctactccaa aaaatacaac
     3121 ctttttatga ttttcattta attgattttt catacttacc cctccctaat atataatact
     3181 tttaccttat acttatattg tactgctact atctcttatt ttgtaaatat atttttaaca
     3241 aaaaaaacag ctaaccttca tttataaggc taactgcttt ttatttttaa gttatatgtt
     3301 atattacaaa tttttactct aaaaaattta cattttttaa gtaacactat aaataattaa
     3361 gtttttatta catccctaat tccttgaact ccctacttcc aggcttaaca actggtaatt
     3421 ttccttcctt acataaagga cactcatcag attcataaac ttgaatatca agttttatag
     3481 cactatatat aggcatacct atatcatgat tagttctatc tgctatacat gcaacaccta
     3541 caacttctcc acctaaggct tctaatactc tttttgtctc catagttgat ttaccagtag
     3601 ttacaacgtc ttcagcaatt attatctttg ctcctttttt aacttcaaat cctcttctta
     3661 actccattgt attgtctttt ctctcagtaa atacagcttc ttttcctaat tgtcttccta
     3721 actcataaga aactattact ccacccatag ctggtcctac tactaagtct atatctaagt
     3781 cttttatttg ttcaacaact gtacttaata cctcagcagc atattctgga aatcttaata
     3841 cttttgcaca ttgtacgtat ctattactgt gttttcctga agataataaa aaatgtccct
     3901 ccaacaatgc atcacttttc tttaatatat ccactacatc tactttactc atattatttt
     3961 tcctccatgt taatgttata aaattattgt ttatttaatc tatatttttt atctttaaaa
     4021 tactatttat tctaccttat ataattcctc ttatttcgtc taaacttttt attccttctc
     4081 tatccataaa ttcattaatt ccatctatta tttcaatacc tattctagga ttcataaaat
     4141 ttgcagttcc aacttggata caagtagctc ctgccattat aaactctata gcatctgtgg
     4201 ctttagttat tccgcccatt cccattacag gtatatttac atttttacac acttcatgta
     4261 ccattctcaa agctataggt tttatagctg gtccagataa cccagcatat acattttcaa
     4321 atactggtct tctatttttt atatcaattg ccattgcttt aaaagtatta actaatgaaa
     4381 ctccatcagc accttcttct tcacaaactt ttgccattcc tacaatatct tctgcattgg
     4441 gtgataattt tattactaaa ggcagtttcg ttatgtttct tacctcacga acaacttctc
     4501 ttgccacttc attttttata ccaaaagcca ttccaccagc tttgacattt gggcaagata
     4561 tatttaattc aataatgtct ataggcttgt catttaacat ttgaacccct aataagtagt
     4621 cttctaatgt cccacctcca acattagcta ttctgactaa gtctagttta ctaaaaaaag
     4681 gtaattcata gtctataaat ccttgaactc cagggttttc aagcccaaca ctattcatca
     4741 taccggatgg tgtctcatga actctcatac cattgttacc aggtttttta tttaaagtaa
     4801 gacccttgct acttatacca ccaagttttt gtatatcata aatctcatta tattcttttc
     4861 caaatccaaa agttccagac gccattatta ctggattttt aaattctaca cttccaaatt
     4921 tcacacttaa attacccatt aaaaatcacg tcctctcccc agaacactgg accatcttta
     4981 caagtctttt tatttcctcc attagtttta cacgtacata ctaaacaggc acctactcca
     5041 caagccatat ggttttcaag agataccata attctcgttt tagtaccttc taccatctta
     5101 actaattttt ccatcattgg agttggacca catgtcaata tataatcata tttctcaaca
     5161 tcaattatat ctgttacaaa agtatttcct actgttgtat agactttatt acatacttgc
     5221 ttatattcat cttctaatat aacatcttct ctaaacccaa gatatgcatc acaatttttt
     5281 atatttttag caaccaaata tagtggagct acaccgattc ctcctcctac tagagctacc
     5341 tttccatcaa ccttagcata tccattacca taagggcctt ctaattttat attatcattt
     5401 acctttaggt tactcaaaat ttgagttcct tctcctacta ctttataaag aaaagttatc
     5461 ccttcttcat ctatatcatg tatacttata ggtcttgaaa gtactggata agtatcccat
     5521 gctcttaaca tatagaattg tcccatttta ccttcaaaat taccttttac tttcattcta
     5581 tacatatctt cgcctatata tatattttcc agaattttat acatcccaaa atctcctttg
     5641 ttttactatt atatgataac tattagtctt catttatacc taaaagtttt attattaaag
     5701 ccattctaac atacattcca tatttagctt gtttaaaata tacagctcta tcatcactat
     5761 ctacatctat gtctatttca tttactcttg gtaatggatg cattactaac atatctttag
     5821 atgccttttc taatttagct ttatttaaaa tataatagtt tttaagtctt tcatactctg
     5881 atttatcttc aaatctttct tgttgaactc tagtcatata taaaacatcc aagcttccta
     5941 taacatcatc caaattatta gtttcataat atgcatgtcc ctttatagct tcttttatat
     6001 aatctggcat ttttaactct tcaggcgcta taaatacaaa ctttgtattt ttatatcttg
     6061 ccatagcctt aaccaaagaa tgcacagttc ttccatattt taaatcacca cataatccta
     6121 ttgtatggct ttctaaagtt ccttttaatg attttatagt aagtaagtct gtaagtgttt
     6181 gagttggatg ctggtttttg ccgtctcctg catttataaa tggcacttcc gtatatctag
     6241 ctgcttctga agctgcacct gattgaggat gtctcatagc tattatatca acataaccag
     6301 atactatcct cattgtatct cctagagttt caccttttga tgcagaggat gaatttggct
     6361 ctgaaaagcc tactacacgt cctcctaatc tgttcatagc agattcaaag cttaatcttg
     6421 ttcttgttga tggttcataa aataatgtag ctaatagttt tccttcacat atacgagaat
     6481 attttgacgg attgtcaatt attttttgag ctaattctaa tatttcatct atttcttcta
     6541 ttgaaaagtc ttctggttgg attaaatttc ttgattttaa catctttcgt cctccttttt
     6601 tagcctctct gaactaaaat taaaagtatt tttgtactgt tagtagctta ccacctttgc
     6661 cctatcctgt caagggattg cgcaaataat catcatatga aaaggctcct atttaacagt
     6721 catattaact tgaatcttga ttagatttaa agtgatacaa aaacgctcta tagtacattt
     6781 aaaatataat cattttttgt ttccatgatt aaattaaata taatatagag cgtttatata
     6841 tatttatgta tttatcaatt tgtcatatta caaaccgatt ataatattct ttttgctgta
     6901 acaaatcttg ctgtatagta tgatgaattt atactagtta cactaactgt ttttcctgtt
     6961 gatggtgaat gtatcatatt acccccacct acatatattc caacatgact aacagaacca
     7021 gaaccattag ttgtgaagaa tactaagtct cctggttgta aatttgcttt acttacagtt
     7081 tgtccatatt tagattgtgc tttagatgtt cttggtatat tctttcctac tgcatttcta
     7141 tacacatatt gtgtaaatcc agaacagtca aaagtgtttg gtccttctgc tccccaaaca
     7201 tatggacaac cttgcttaga atatgccaag ttaagtacag cttgaactgc attagagtta
     7261 gctgttggca catttgaatt attattttgt tgattagtat ttgcattgtt ttgttgatta
     7321 ttatttgtag aatctacatt ttggtcaata cttgggttta cttgtggctc ttctattaca
     7381 ggagctttat cagatatata agatgatttt acatatcctt ctgtttcccc atctttaact
     7441 tttaaccaat ctccattatc ttccaaaacc aataaagaac ttcctttttc cagctcttca
     7501 attacttctg aactttcact cttatctttt cttaagttta ctttatcagc gttagtatat
     7561 cctacacctt cagatatatc tacatatcta gtagctaacc aaccttcgcc atcttcaact
     7621 ttaaccttaa cccattctcc ttgagttcct aaaaccgtaa attcatctcc agaataagct
     7681 atgttttgaa cttgaccttc ttctcttatc tttatagcta ctccatcttt tatttttgca
     7741 actttatatt ttacttcttt atattcacct aaatttaatt cagtatttgt tttatcatcc
     7801 ttattttgag atgaatcatt tacttcatct gcatttacaa ttgaacttac tgataatgcc
     7861 ataactgatg caaatactgg tattaatatc tttttcttca cgataaacct cctaaaattt
     7921 tcccataatt atcttatgat tctattttgt aggtgaagtg tacctaagga taaaatacta
     7981 atacacaata cattaataaa agctctatct gtaacttttt tgtaaaccac tttatataac
     8041 ttttatagta ttattatata ataaaataaa taaattagct tatccaatat gtcacctaaa
     8101 tatacattat ttacttaatt atataatatg ggtctgtaaa agtaaagaat tttgtaccat
     8161 tcgaggttaa agtgcgcata aatattgttt tatgtgtttt tttatgcgct tttgtatggc
     8221 gtatattttt tgatggattt agtataaagt tagatattct gtgaaatttt taaccctaca
     8281 taaatca
//
LOCUS       DAGSHT010000148         8253 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGSHT010000148 DAGSHT010000000
VERSION     DAGSHT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.153"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..175)
                     /locus_tag="MJB72_002809"
     CDS             complement(<1..175)
                     /locus_tag="MJB72_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263885.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDT"
     gene            complement(176..592)
                     /locus_tag="MJB72_002810"
     CDS             complement(176..592)
                     /locus_tag="MJB72_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263886.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(660..1073)
                     /locus_tag="MJB72_002811"
     CDS             complement(660..1073)
                     /locus_tag="MJB72_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263887.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(1906..2535)
                     /locus_tag="MJB72_002812"
     CDS             complement(1906..2535)
                     /locus_tag="MJB72_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBY8263888.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2614..3576)
                     /locus_tag="MJB72_002813"
     CDS             complement(2614..3576)
                     /locus_tag="MJB72_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY8263889.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(4338..7934)
                     /gene="cbpA"
                     /locus_tag="MJB72_002814"
     CDS             complement(4338..7934)
                     /gene="cbpA"
                     /locus_tag="MJB72_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBY8263890.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAGGDTNVGNPEGDANAGNAGGDANAGNAEED
                     ANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLI
                     IDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQG
                     ILYLWFSDKFTGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEE
                     TMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDN
                     GGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNI
                     NIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKP
                     DINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         2640 a         1510 c          876 g         3227 t
ORIGIN      
        1 tagtatcgaa tactttagcg atatccatag ctaacttcaa agaaggatta tatttcccat
       61 tttctaagtg tacaatagtc tcacgtctta ccccaaccaa tttagcaagt tcattttgcg
      121 tcataaggag tttttctctg tattctttaa tttttgtttt catcaatatt gccacttagt
      181 tacatatccc ccttttatca aagtatatat acaacactaa tttagataaa gactgtataa
      241 ataaaactgc aatgaggatt atacctgtat ctaggtttga tatatttata ttacttgtat
      301 aaggtgttgc aaagaaaata gatattatta ctatagaata tatcattatt tttattgcaa
      361 tcttattaac ctttgatagt ataaattcag atgattcatc aactaaatca ttatattttt
      421 tattataaat tgaataacat ccaaaaacta ttaaccaact caaccctgga agagaagcag
      481 tgcttggacc tttaaaaata tacttagtgg ctaatacagc tataaaaaaa taagttatta
      541 caagcaactc caggataagc tttgatctta caccagtttc tgatttagac atataaaatc
      601 actccttaaa aattatattc tctatactct ttaattttcg tttttatcaa tattatcact
      661 tagttgcata ttccctttct atcaaaatgt atatacaaaa ttaatttaga taaagattgt
      721 ataaataaaa tagctaagag aattacacct atatccaaat ttgatatatc caaacctctt
      781 gtataatttg ctgaaaagaa aacagctatt agcaaaatag aatataacat tatttttgat
      841 gcaatatgat caacctttaa taacataagt tttgacgcct cgtcaactaa atcattatat
      901 tttttattat acatgaagtc acatgcccaa aaagcgaata aaattataaa agtaatacaa
      961 agatttccct ctttaaaaat aaactttttt aataatgagt ctataaaaaa ataggatatc
     1021 ataagtaact ctgaaacaag atttgtttta acaccaagct ctgatttaga catataaaat
     1081 caccccttaa aagttatata tttataacac cttatgttat aaatatataa cttttccaaa
     1141 taaattgcaa tagaatattg aaaatattca ataatatctt cataaatata ttttataaaa
     1201 gattttataa taaacattac aatacagtac taaattagtt tatttcatct atcatctcaa
     1261 tcatatcatt tactaactat tattctaatg agcatataac attgaaactt ataattatac
     1321 aacctaaatg cctcccaatg taccctcaaa acatcatttc ctgtaactga agtataattg
     1381 gtatatttta tcactgaaat aaaaaaacac tagtcaataa agactagtat tttcaagtat
     1441 tttatcattc tttttttaat tattggagac gaatcatcag aataatgccc ataatttatc
     1501 tatatgcaaa ttctaaattt aaaataaagt ttcagataaa tttactattc tatgctatga
     1561 tacaacagaa aaaactgaaa tctatgagtg gtagcttcat ataatcctag ttcctacagt
     1621 aatcaaaact gaggtttatt ctatatgtaa ctaatgataa attttattat aggagttatt
     1681 agatctattt gtgttactat aagtagtaat tatatatatg ataatttttt aacacttttc
     1741 tgttggctat atagaaagtg gtttagaatt taaatttaaa taaaatttaa attcaccaaa
     1801 aagaaatcta tattagaaac cactactcta ttccaccaga ttgtaatttt ttgttcttta
     1861 tatcacaatg ctataccata ttaaattgaa aaataagttt taaatttatt tgttacaatt
     1921 cattaattca aaaaaatatt ttgagtcatg agttttgtaa agatttagcc atgaatttaa
     1981 tgcttctgat tcaaaaacat ctaaaagttt caatacttga aacgtaaatt ccaacggagc
     2041 aattcctgaa gccgtaacta aattttcatc actaactaca gattccgttt tataatattt
     2101 ttctccagag taatttggta ttaccatttt aagaaactct aagtcattac ttgtatgatt
     2161 tctaaaatct aatagccctt ttttagctag gcctaatgta gctccacata tggctgcaac
     2221 tatagaatct tgtcttaaag cttctttaac tttttctaat attatctcat gattcataga
     2281 taaccaagta tttccccctg gcaaaattaa taaatcctta ctttccatat taaactcatc
     2341 aactgtaatt tcaggtaaaa cttttaaccc acccattgtt ataataggat ttttatcaat
     2401 tcctacagta attactttta gtggctttaa tcctttttta aaatatcttc ctgtatttaa
     2461 ttcagcagtt aaatatgcca cttcccaatc tgacatagta tcaaatacat acatatatac
     2521 ttttctattt ttcatctttt ttactcctta tgtaattaat ttatatatct aattataaaa
     2581 taacttaact gacatattca gtcagttaag ctattaaatt tgataataat caattaattc
     2641 tagtaaatct tcaattattt tcttcctaag gcttagtggt tcaattactt gtattgtctt
     2701 tctataaggt aaaagtatat ttggtatgta cttgcatatt acttcctctt caagcataaa
     2761 tattatttta ttaggtgttc tttctttcaa gtagtgccct aaaaaccaat gttggcttaa
     2821 actatctaaa gaatctatac tcccttctat aactaattga attatatttt gttcatcttt
     2881 tatttcaggt ataattcctt ttataaaaaa ttcacttgct gaaaattctg tcggtctgtt
     2941 aaatgtattt gatgtttcta tgatttcact aatcctatcc actctgaaac tacgaacctc
     3001 atttctcata ttacaaaatg caactacgta ccaattatta ttccagtgaa caattccata
     3061 tggatttata attctatatt ttgattttac tttacgacct gcatgatagt tgatttctac
     3121 agatagctcc tttaatatac tattctccaa ttttttaagt actgattcta tagacacttt
     3181 cccaattggt tttattactt caagggctct taagtggttt gttaaaatat cttcttgttt
     3241 ttgatttgaa tacattttta actttaatat cgatttatct aatgcttcgt ctaagaagta
     3301 tccttctttt ttagcaaata gtgctgcatg aagtagagat ttttgctctt ctatatcaaa
     3361 aaaaagaggt gctttagtaa aattatttaa aaggctatac ccaccattat gacctgtatc
     3421 tgaaattata ggtactccac tcgcagaaag tacatcaata tatcggtaca ctgttcttat
     3481 atttatttct aatttttcag ctatctgttt tgctgtaatt tttttgtcag agtttaacat
     3541 ccacagtata gacatcatat tgtcaatttt agccatgtag tttcacctct ttctatttgt
     3601 tatataaagt tattatataa ctttttgatt gattttttac aaaaaaacat tagtaaactg
     3661 ctaataattt atactaagat ttatttcaaa tatttataga tagtcttttt ctatagttaa
     3721 gttatccatt aaattacata gtagtatatc tattttagtt gatatatttt cagctacctc
     3781 ccttgaatat tattacaata agtctaaatc ttctttcttc cataaatata ttatgaggaa
     3841 agactatcat ctttaatggt ttaatagtat aaaagatatt aatttaaaaa aatctatttt
     3901 tatatcagat gggtattgtt ttttataaaa ctgaaaaaat cacctaatag aatctatgat
     3961 tttttaacat aacctccata aattagttcc aattatcact tcaaattcaa atttttatct
     4021 gaagaatata caatcatatt tccatactca acataatatt gagtgtttgg cttgtattta
     4081 caaattatta ttctaacaaa aacaactata catagattaa aatcatcttc taaacataac
     4141 tactaaaatt gaattaatga tatcaattcc attaatctct tttaataaaa aattctcttt
     4201 tctatatgaa aattatttca aaacttttaa gttaaataca aaaatgacta taaatagatt
     4261 tttatttaca caaatctatt tatagtcact atagtttatc caataaaata ataaaataaa
     4321 tatgtacaca ataaatgtta tttacgtctt aagtatttat ttgttagatt tttaattaaa
     4381 aaaaacaagc taatgaacat taatgctaaa tctagcatta tattgctttg atctcctgtt
     4441 tgtacatttt gacctacatt actatctaaa ctttcatctg tatctatatt tatatctggt
     4501 tttccattcc catctgtatc tatatttata tccggttttc catctccatc tatatctata
     4561 tttatatctg gttttccatc tccatctgta tctatattta tatctggttt tccatctcca
     4621 tctgtgtcta tatttatatc tggttttcca tctccatctg tgtctatatt tatattcggt
     4681 tttccatccc catctgtatc tatatctggt ttgctatttc ctccattatc tgggtctggt
     4741 ttactatttc caccgttatc tggatccggt ttgctatttc ctccattatc tggatctggt
     4801 ttaccattgc caccgttatc tggatctggt ttgctatttc ctccgttatc tggatccggt
     4861 ttgctatttc ctccattacc tgaatccggt ttgctatttc ctccattacc tgaatccggt
     4921 ttgctatttc ctccattacc tgaatctggt ttgctatttc ctccattacc tgaatctggt
     4981 ttgctatttc ctccattatc tggatctggc ttatttagat ttggattacc tgtatcataa
     5041 cctcctgagc cagtatctac atttttatca ggcttccaat aatcatctac tccatcatta
     5101 tctgtatcta ttgctggacg attataatct gtatcagggt ttccatctcc attggtatct
     5161 atatttttat caggtttcca aataccatct ccatttccac cttctgtact aggcttccat
     5221 tcacctgtat tatcagtatc tatgttaata tctggcttcc aatcaccact cgtatctata
     5281 ttgatatctg gccttccatc accatcagtg tctatattta tatccggtat tccatcccca
     5341 tctacatcta catttgtcat atctaacttt aacttctttt catcctcact accttcaatg
     5401 taatttatat catctccaat atattttaca gtaatagttt gttcagtagt aaaaatatga
     5461 tgattgctat ctaaaggtct ccattcaata gatgcagtac tataattatg actctcacta
     5521 cccttattat gagtttttaa ttctactgga tctttatatt cctttccatt aatatagaat
     5581 tgtacgtaac ctcttggaga ggcacaatct ggtccttctt tcttatctgg tgaaatagta
     5641 acatttaatg taaacgtatc tgttggttta aaaacatcag ttttagcttt agactcttct
     5701 acaactaatg aagtttctgt atcagcagga ataatttctg ccttataata tgctctatgc
     5761 ccccaataag catccttaaa aggatatata ctcgaaaact gtgttgatgt aatgcttaat
     5821 tgatatttcc cagcatcaac ttgttttgta agatcatcac ttcctaaatt tatatctggt
     5881 tccatcgttt cctcatttaa acgttgagaa ctaatagtca taaatttttt atctgtgagt
     5941 ttttcacctt taggagtagt tattggaata ccaccatctt ttaatagcca atctaaaaat
     6001 gatttttcta cgtcttcttc aaatggtaat ccatcatatc ttttaataat caaatatggt
     6061 ggagtatcat caatagtaaa ttcttcatat tgctttaaaa attgagaacc tgaacctata
     6121 tctttaatat aaaaatcatc tggttttaga acttctccag taattttatc tataacaact
     6181 aaattaacag atacttcaga tccagtaaat ttatcagaga accataagta aagaatccct
     6241 tgagcatctg tataactagg tgctccataa ggataaattt ttccatcaat tagcatttta
     6301 aaatcatcaa ctagtgtagt actaacttta tcttgcccta aatacgttag agatatttta
     6361 ttcataaaaa cttttgtttt cttttctaaa tcaccgtatg caactgctcc acctaaactt
     6421 ttgaatgttg ttgcttttaa tgaaccacct gttactataa catctcccgt tgtagcactt
     6481 aggtcaaaaa tccaatctcc cactcctgtc atataaggta aaatcgttcc tccagttaca
     6541 agtatacgat atcctctatt tgttcctgga ttaacacatc catccccaaa tgcactacca
     6601 tgtgcacctc catatgaata ggaaagtcca ccattaattg taatattccc actataacca
     6661 gaacctgtgc taggatctac tactgaggga cttaatgcat tctttgttgt tgtatagtat
     6721 cctccaccta ttccagctcc atggtatgca cctttagcag ttatagtacc accattgatt
     6781 gtgatatctc caccactacc attaccgcct ccacctattc cagctccata tcctgtatta
     6841 cgactcgatt cttgacctat agcagttata gcaccaccat taataataat atttcctcca
     6901 ctttcagtat ttccaccgcc aatacctgca gaataataat ttccaacaac ttttaaactt
     6961 cctctattat tactatctag tagtgtaagg cgatctcctt tttttaactt gtctccattt
     7021 tctagagtta catctctagg aatttttcca ccctcaggaa ctataaactc gccttcaata
     7081 tctctattta caacagaatc atcaataatt agagtagtac cttttcctgc tctcatacct
     7141 ggataatata catcacctga ctctagcttg ttttcactac catcttgtaa tataacatgt
     7201 gcatatgcac caccatcttt atctggtgtt aaagtaaatg ggctattaga tgcagtttta
     7261 atatttactc catcaaatat taattcagct tttacatctt taggtataac aatcctatgt
     7321 gttgttggag tatttggatt attattttga attgtcattt gttttccact aagaatagtt
     7381 aaaacgccac ttgcaaattt aaaatcgaca tttatttctc caccactaat tttaaagtct
     7441 cctacagatg taggatcttc tgcagtggct tcttccacaa ctaatgcatt catatctcct
     7501 tctgcatttc ctgcattaga atcttcttct gcatttcctg cattagaatc ttcttctgca
     7561 tttcctgcat tagcatctcc ttctgcattt cctgcattag catcttcttc tgcatttcct
     7621 gcattagcat cttcttctgc atttcctgca ttagcatctc ctcctgcatt tcctgcatta
     7681 gcatctcctt ctggatttcc tacattagta tctcctcctg catttcctgc attagcatcc
     7741 ccttctgcat ttcctgcatt agcatcccct cctgcatttc ctgcattagt atctccttct
     7801 gcatttcctg cattagtact ttcttctata gtatctgcga aagtatttga cgcattaaat
     7861 gatgctactg atccaagtcc cattgagata cttaaaatca ttgatagtgc tttcttttta
     7921 catttttgtt gcataaattc cccctcccct taataatttt taatatacac tgtatacatc
     7981 cttatataca ctctaataat tcttaatata cactgtatac atccttatat acactctaat
     8041 aatttttaat atatacttaa aacttatatt tgtcaatgaa tatacagttt ttttcaacct
     8101 tatatattat aagtattcat tatttgctaa ataactacaa taaaaatttt aattatataa
     8161 aatttattta atcttttata taataataca aggggtaatt aattgaaaaa actagtaaaa
     8221 atattaataa atattatttg cattattacg cta
//
LOCUS       DAGSHT010000149         8234 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.154, whole genome shotgun sequence.
ACCESSION   DAGSHT010000149 DAGSHT010000000
VERSION     DAGSHT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.154"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..2259
                     /gene="cas3"
                     /locus_tag="MJB72_002815"
     CDS             <1..2259
                     /gene="cas3"
                     /locus_tag="MJB72_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBY8263891.1"
                     /translation="EILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESD
                     KKSLKKIARHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQ
                     EEIYEYIEKGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSD
                     YNNGQIDDSVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEI
                     YKSQGNLEKNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNK
                     ISAESFYRKNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLS
                     INFCKPCVIATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQ
                     FACKYLDVDFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEIC
                     EGKDVKVSFIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVD
                     IDVLHSRFKFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQR
                     MGRCNRNNKYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDD
                     YYDNSVVKKYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKI
                     FRDVDVSYKIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSK
                     FEYNSTVGLILK"
     gene            2700..3647
                     /gene="prmA"
                     /locus_tag="MJB72_002816"
     CDS             2700..3647
                     /gene="prmA"
                     /locus_tag="MJB72_002816"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBY8263892.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            3666..4424
                     /locus_tag="MJB72_002817"
     CDS             3666..4424
                     /locus_tag="MJB72_002817"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBY8263893.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            4426..5724
                     /gene="mtaB"
                     /locus_tag="MJB72_002818"
     CDS             4426..5724
                     /gene="mtaB"
                     /locus_tag="MJB72_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBY8263894.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            5767..6117
                     /locus_tag="MJB72_002819"
     CDS             5767..6117
                     /locus_tag="MJB72_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBY8263895.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            6280..6459
                     /locus_tag="MJB72_002820"
     CDS             6280..6459
                     /locus_tag="MJB72_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBY8263896.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            6489..6932
                     /locus_tag="MJB72_002821"
     CDS             6489..6932
                     /locus_tag="MJB72_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBY8263897.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            7043..7540
                     /locus_tag="MJB72_002822"
     CDS             7043..7540
                     /locus_tag="MJB72_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBY8263898.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            7733..>8234
                     /gene="raiA"
                     /locus_tag="MJB72_002823"
     CDS             7733..>8234
                     /gene="raiA"
                     /locus_tag="MJB72_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBY8263899.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDN"
BASE COUNT         3448 a          742 c         1550 g         2494 t
ORIGIN      
        1 gagatactaa aaatatgttg tgctttgcac gatttaggaa aaattaattc aattatgcaa
       61 caaaaaatgg agattaataa taaaatatcg tatagttgct cagaagaaga gaggaaaaag
      121 ttagagtctg acaaaaaatc tttaaaaaag atagcaagac ataatatatt ttctggagca
      181 tttttaaaag atatattaga aaaaatgaac ttgtcagaag aagataagtt atatatatat
      241 aaaagtataa tgttacatca tggaaactat gaagattata tgaggttaag tattagtaaa
      301 gttcaagaag aaatttatga gtatattgaa aaaggcattt tagaaaaaga agagtttaat
      361 ctgaaagata tagaaagtta tataaatagc attttaaatg tagactttaa atttggtgaa
      421 gatgtattgg attatgatta catagataaa ttaagtgaaa gtatgtttat agacagtgat
      481 tataacaatg gtcaaataga tgattcagtt ttaaattata gaaagtttaa gtatatttta
      541 tataaaggga tgctgaactt aatagaccat agtgcatcaa gtagacagaa aggtataaaa
      601 ttttataacg attttactga tgaagaaatt gataatatga ttttaaatga gatatataag
      661 agtcagggta atttggaaaa aaataatata gagttcaata ctatacaaaa aaggttgaga
      721 gaactttctg gaagaaatgt tctgactgtt gcatttacag gttcaggaaa aacagctgca
      781 gattatagaa agacttttaa aagaaaattc tttttagtgc caaataaaat atcagcagaa
      841 agtttttata gaaaaaatat ttttcaaaat aaaaatgatt tagatacaag aagtagtaat
      901 gattacattg gattacttca tggagatata aacttatatt cagagaatga agataatgga
      961 gaacatgatt ttgtattgac attaagggat atagatttga gtataaactt ttgcaaacct
     1021 tgtgtaattg caacagttga tcagttatta cttagtatgt ttaaatttcc gggatatgaa
     1081 aaaatattcg cagccgtaaa aaatgcatct ataactgttg atgaagtaca tttattggaa
     1141 ccaaagatgt ttttgatttt gatatatttt atgcagtttg catgcaagta cttagatgtt
     1201 gattttcact taatgacagc tacacttcca aaatcatata aagagcaaat gataaacaag
     1261 ggaattattt ttcaagaaga aagtaatgaa aatgttactg ataaaggaga aatagttttt
     1321 attgagagca acaaggaaga agagatttgt gaaggtaaag atgtaaaggt ttcttttata
     1381 aaggaaaaag aaatcaagtc aatagttgaa gatgctttag aaaataaaca aaaaattctt
     1441 ataataaaga atactattga ttcagtcaat agaacatatg agtttttaaa agaaaattta
     1501 tctgataagt atggtggtgt agatatagat gttttacata gtagatttaa gttcaaagat
     1561 aaaaaagaga aatatagtaa aatattaaat ggagagggtg atatttggat atcaactcaa
     1621 tcagtagaaa tatccttgga tttggatttc aatataataa taagtgattt agcaactatg
     1681 gatagcttaa tacaaagaat gggtagatgc aatagaaata ataagtatga atatggaaac
     1741 ttttatattc tacctagtga agataaaatt tatgacaaca aacttaagag cacaactaaa
     1801 aacatcttaa aggacatatt aaaaactaac tctattttta caatgggaat tagaaaaact
     1861 attttggatg attactatga taatagtgta gtcaaaaaat attttgaaga gaattttata
     1921 agttgtgata aagaaattaa gaatatttat ggtgtaaata aagaaatttt tgatggattg
     1981 gatttaattt ttaattttga accttacaaa aatattgttg atagtaagaa ggaagcagct
     2041 aaaattttta gagatgttga tgttagttac aaaattattc tggaagaaga tttttataag
     2101 gaagatagag agttacaaca agattcgatt caagtatcag ggtttatatt taatagatta
     2161 tattattatg ggttaataag caaagtagaa ggatacatgg tactaaaatc tagtagtaag
     2221 tttgagtata atagtactgt aggcttaatt ttgaagtgag catatgttta aatataatat
     2281 acctttagac attgaaaata caaaaagtac tactgtaagc tcactgtaaa aactattttt
     2341 taaaaggaaa aataagttgt gagatagagt gtttacaagt gtatatagat aataaatttg
     2401 aggtttaaga ttaactaagt gggtttcagg tacatcgcga aaataattat aagttggaga
     2461 ttttgtatgt ttaagattaa ctaagtggat ttcaaatatt gatatagtag atataataga
     2521 agttttatta ttaggtttaa aattaactaa tcggattttt aaatattctg actattatat
     2581 aaggatatta ttagattgaa tttaaaacca ataatataaa acaacaaaat attatattta
     2641 tgatataata aaagttatgg aaaaacagga atattactat agagaagaga ggtaaactta
     2701 tgaataattg gatagaagta actataaaaa caacaacaga agcagttgaa cctataacga
     2761 atatactata tgaacaagga gctggaggag ctgtaataga agacccaaaa gactttttat
     2821 tccaaaagaa aaatgaacta gactgggatt atgttgaaga agaagtattt aaaaagaatg
     2881 aagaagatga tgttcttata aaaacttatg tttctgaaga aaaaaatgtg atggaatttg
     2941 tcgaaataat aaagcaaaaa gttttaggat taaaagattt tggtatagat ataggtgaag
     3001 gaagtgtatc attggaccaa gtaaatgaag ctgattgggc aaatgcatgg aaagcatact
     3061 acaaaccaac aaaagtagga caaagggtag tagtaaagcc tacatgggaa gattatgcta
     3121 tgcaagaggg ggatttaatc atagagcttg accctggaat ggcatttggt acaggtacac
     3181 atgagactac tagtatgtgc attagagaat tagaaaagta tgtgaataaa gattctaaag
     3241 tatttgatat tggatgtggt agtggaatcc ttgcaatagc agcagctaaa ttaggagcaa
     3301 aagaagttgt agctgtagat ttagatgaag tggctgtaaa agttgcaaaa gaaaatgttc
     3361 ttgaaaataa agttgaaaaa agtgtatctg taatgcatgg taatttaact gatgtaataa
     3421 aagataaagc ggatgtaata gttgcaaata taatagctga tataataaag atacttgcaa
     3481 aagatgttca aaactttatg aaagaagatg ctatattcat atcttcaggt ataattttgg
     3541 ataaagtaga agaagttaaa gaaagcttaa ttgaaaatgg atttgaaata gttgaagttc
     3601 aaaaattagg tgaatggtca gcaatagttt ctaagcttaa aaaataaggg atatggtgac
     3661 gtataatgga tagatttttt gttgaaaaaa ataatataaa tctacaagat aaaacttgca
     3721 ctatagaagg tgaagatgta aaacatattt caaaagtttt aagatgcaaa ttaggtgaga
     3781 aacttgaaat atgtgacaag aataataatg agtatatttg tgagataatg aatatagata
     3841 agagtatagt taatttagaa attttagaaa aagttgatat aaatagagaa tcagaactta
     3901 aagtaaggct ataccaggga cttccaaaag ctccaaaaat ggaaataata cttcaaaaat
     3961 taactgaagt gggtgtagaa gaaataatat tagttcaaac taagagaagt gttgttaaag
     4021 ttgatgacaa aaaagaagat aaaaaatttg aacgttggga aagaataatt tatgaagcag
     4081 caaagcaaag caaaagaggt aagataccaa aattaagagg tgttttaagt tttaaagaag
     4141 ctttagaaga tatgaaaaaa aatagtgtaa atatttgccc ttatgaaaat gaaagaacag
     4201 tttcaataaa acatgcacta aaaaaatgtg attctaatat agatagtgtt ggaattttta
     4261 taggtccaga aggtggattt tctgaagaag aaatagaaca aatacaaaaa aataattgta
     4321 atgttgtatc attggggcct agaatattaa gaactgagac agcttctgtt gtagcatcaa
     4381 caatagcttt gtatgaacta agtgatttag gaggagaaaa ataaattgaa aaaagtagct
     4441 ttttatacac ttgggtgtaa agttaatcaa tatgaaacag aagctatgct agaactattt
     4501 gagaaagatg gttatgaaca agtcaatagt gaagagtatg ctgatgttta tgttataaat
     4561 acatgtacag ttactcatat gagtgataga aaatctcgtc aatatataag acgagttaaa
     4621 aagaagaatc cagatgcaat aatagccgtt gtaggatgtt attctcaggt atctccggaa
     4681 gaaattttgg atatagaaga agtaaattta gtcatgggta caaatgatag aagaaagatt
     4741 gttgaagaaa ttaaaaagat aaattcaagt aagaaagtta gtacagttga tgacataatg
     4801 aaagttaaag cttttgaaga aatagaaatc agccaaacta atggaaagac tagagcattt
     4861 atgaaaatac aagatggatg tgacagattt tgtacttatt gtatcattcc ttatgcccga
     4921 ggacgcgtta gaagtagaga tattgatagt atagtcgatg aagttaaaaa attggctaat
     4981 aatggctata aagaggttgt attaacagga atacatgttg cctcttatgg aaaagattta
     5041 aaggataggg atattaaact tcttgatgta ataaaacaaa taaaccagat agaaaaaata
     5101 gaaagaatta ggcttagttc tgttgaacca attttattta cagatgaatt tgtaaatgaa
     5161 gttttaaaga tggataaagt ttgtcctcat taccatttat cacttcaaag tggatgtgat
     5221 gaaactctaa aaagaatgaa tagacgatac acaactttag agtacaaaac tattgtagat
     5281 agattgagaa gtaaaatgcc agatgtggca ataactacag atgtaatagt aggttttcct
     5341 ggagagacaa atgaagagtt taaaaaaacg tacgagtttt taaaagaaat agagctttca
     5401 caaatgcata tttttaaata ttcaccaaga aaaggaactc cagctgcaac tatggaaaat
     5461 caagttgacc ctcaaatgaa acactttaga agtgaacaac ttttaaattt aagtaaagta
     5521 aattttaata aatttgcaac taaatttatc ggccgtgagt tggatgtttt atttgaacaa
     5581 aatatagaag gcaataaata tgagggatta acttcaaatt atataagagt tgttgttgaa
     5641 agtgacaaaa atatacaagg tcagatatta aaagtgaaaa taaatgatgt aaaagatgaa
     5701 tatgtagaag gaattttgct ataacatgta gaaattataa tatttataag ataggaggtg
     5761 gaaagaatgg attgtatttt ttgtaaaata gcgaacggag aaataccttc aactaaagtt
     5821 tatgaagatg atagagtctt agcttttaat gacttgaacc cagttgctcc atatcatata
     5881 ttagttgtac ctaaaaaaca ttatgatagt ttaattgata ttcctgataa agagatggac
     5941 attgtgtcac atattcatgt tgttattaat aaaatagcta aagagaaagg ttttgaccaa
     6001 actggattca gagtcataaa taattgtgga agtgatggag ggcaagaagt taaacatttg
     6061 cactatcata ttttagctgg taaaaagctt cctaattacg aagcgggaca aaactaaact
     6121 aaattattga aaaattgagt tccttcttga taaaaatact tgataaaagt aaaaatttag
     6181 tttataataa acaaagtgtt aggttgtaat gcatattatg caaaaagaac atagttacag
     6241 tcccagcata attgcgctat tggagggagg gaaaaagaaa tgtcagaagt aagagttaga
     6301 gagaatgaaa cattagacag cgctttaaga agattcaagc gtcaatgtgc aatgtctggc
     6361 atcatgtcag aagtgagaaa aagagagcac tatgataagc caagcgttaa gcgtaagaaa
     6421 aaagctgaag cagcaagaag aaaaaacgct aagaaatagt agtaaataag aataaagagg
     6481 tgaaggggat gtcccttaag caaaagttac aagaggattt gaaatcttcg atgaaaaaca
     6541 aagatacagt gagaaaatct gtagtaactt taataagagc ttctataaag cagtatgagg
     6601 ttgataatag ggtcgagctt gatgaagatg gaattatcga tgtaatagca aaacaattaa
     6661 agcaacgtag agatgcatta gttgaatttg aaaaagctgg aagagaagac ttaataaaag
     6721 aaacagaagg tgaaattgaa gttttaaaag agtacctacc tcaacaatta agcgaagaag
     6781 agttagaaga aattgtaaaa tctactatat ccgaagtagg ggcaacttct atgaaggata
     6841 tgggaaaaat aatgtcagtt atccaaccaa aagttaaagg aagagctgac ggaaaactta
     6901 taaataagtt ggtaaaacaa aacttacaat agattgatat agcctagaga gaaatctcta
     6961 ggctttttat agtttttggg aataacgagt catatttgag catatctata atcaaagaaa
     7021 attttgaaag aaggggaaaa atttgattat atcagtaaaa gagattagga attttttgat
     7081 aaacatattt ataccactaa taataggtta cttaagtagc atcttaacaa tgattatcag
     7141 tggaacagat atttcaacct attatttaca actcacaaag ccaagctttg caccaccagc
     7201 ttttatcttt cctatagttt ggactatatt gtacatattg atgggtatat cttcatacct
     7261 aatattaaga aagggttaca acttacctaa agtaagagat gcaatttttt attatggatt
     7321 acaacttgta ttaaacttta tttggagtat actatttttt ggatttggac ttagatttac
     7381 agctttgata gatataataa taatgatttt ggtttctata ataatgattt cgaagttttc
     7441 taaaatagat aagagatctg gatacataaa tttgatttat ttaatgtggc ttgtttatgc
     7501 aggatttttg aactatttta tatggtttat taataaatag aatgtaaaga ttttgtaaat
     7561 taccctgtac ttttaataat atcataacaa acgaaaaata aatagactat taaaaataat
     7621 cattttcata tacaataata ttaatttaaa tacaataaat ttataaaaat tgtatttaaa
     7681 gattaacata ttaaagtatt agaattggaa aggcttgaag ggagaggttt ttatgcaaat
     7741 aatagtatct gggagacaaa tgaaattaac tgacggaata aaaggatacg tagatggaaa
     7801 attaagtagg ttagaaaaat accttgatcc tgaatcagaa gtgaaagtaa cagtaagtgc
     7861 gaaaaaggat agacaaaaag tggaggttac aataattcct attaacggtc aaataataag
     7921 agcagaagat gtagaagatg acttatatgc agcaattgat attgtttgtg acaaattaag
     7981 tagacaagtt gtcaaatata aaactaaagt taaagacaaa gttcaaaata ataagagcat
     8041 aagatttgaa aatcttgact ttatagataa tagcagtgaa tttgatgatt atgattatga
     8101 tgatgaagaa gatgaaaata tagtaataga aagaagaaag aaatttaatg taaaacctat
     8161 gagttctgag gaggcgatac ttcaaatgga actagtagga cacaatttct atatgtttag
     8221 aaaccaagat aatt
//
LOCUS       DAGSHT010000150         8194 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGSHT010000150 DAGSHT010000000
VERSION     DAGSHT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.155"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..2922
                     /gene="tcdB"
                     /locus_tag="MJB72_002824"
                     /gene_synonym="toxB"
     CDS             <1..2922
                     /gene="tcdB"
                     /locus_tag="MJB72_002824"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBY8263900.1"
                     /translation="EVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKILM
                     LNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLIS
                     KVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKLN
                     SVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANFI
                     ISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGDI
                     SSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYINE
                     KINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNFS
                     DKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSLY
                     YFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFST
                     EDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYFS
                     PETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKHF
                     YFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTA
                     VVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFSD
                     SGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGL
                     VRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEKG
                     IMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFAH
                     QNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVIS
                     E"
     gene            3044..3544
                     /gene="tcdE"
                     /locus_tag="MJB72_002825"
     CDS             3044..3544
                     /gene="tcdE"
                     /locus_tag="MJB72_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBY8263901.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            3623..3784
                     /locus_tag="MJB72_002826"
     CDS             3623..3784
                     /locus_tag="MJB72_002826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263902.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            3849..4055
                     /locus_tag="MJB72_002827"
     CDS             3849..4055
                     /locus_tag="MJB72_002827"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263903.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            4272..>8194
                     /gene="tcdA"
                     /locus_tag="MJB72_002828"
                     /gene_synonym="toxA"
     CDS             4272..>8194
                     /gene="tcdA"
                     /locus_tag="MJB72_002828"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBY8263904.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTN"
BASE COUNT         3296 a          874 c         1286 g         2738 t
ORIGIN      
        1 gaggtaaatg gaagtaatgg atttgtttct ttaacatttt caattttaga aggaataaat
       61 gcaattatag aagttgattt attatctaaa tcatataaat tacttatttc tggcgaatta
      121 aaaatattga tgttaaattc aaatcatatt caacagaaaa tagattatat aggattcaat
      181 agcgaattac agaaaaatat accatatagc tttgtagata gtgaaggaaa agagaatggt
      241 tttattaatg gttcaacaaa agaaggttta tttgtatctg aattacctga tgtagttctt
      301 ataagtaagg tttatatgga tgatagtaag ccttcatttg gatattatag taataatttg
      361 aaagatgtca aagttataac taaagataat gttaatatat taacaggtta ttatcttaag
      421 gatgatataa aaatctctct ttctttgact ctacaagatg aaaaaactat aaagttaaat
      481 agtgtgcatt tagatgaaag tggagtagct gagattttga agttcatgaa tagaaaaggt
      541 aatacaaata cttcagattc tttaatgagc tttttagaaa gtatgaatat aaaaagtatt
      601 ttcgttaatt tcttacaatc taatattaag tttatattag atgctaattt tataataagt
      661 ggtactactt ctattggcca atttgagttt atttgtgatg aaaatgataa tatacaacca
      721 tatttcatta agtttaatac actagaaact aattatactt tatatgtagg aaatagacaa
      781 aatatgatag tggaaccaaa ttatgattta gatgattctg gagatatatc ttcaactgtt
      841 atcaatttct ctcaaaagta tctttatgga atagacagtt gtgttaataa agttgtaatt
      901 tcaccaaata tttatacaga tgaaataaat ataacgcctg tatatgaaac aaataatact
      961 tatccagaag ttattgtatt agatgcaaat tatataaatg aaaaaataaa tgttaatatc
     1021 aatgatctat ctatacgata tgtatggagt aatgatggta atgattttat tcttatgtca
     1081 actagtgaag aaaataaggt gtcacaagtt aaaataagat tcgttaatgt ttttaaagat
     1141 aagactttgg caaataagct atcttttaac tttagtgata aacaagatgt acctgtaagt
     1201 gaaataatct tatcatttac accttcatat tatgaggatg gattgattgg ctatgatttg
     1261 ggtctagttt ctttatataa tgagaaattt tatattaata actttggaat gatggtatct
     1321 ggattaatat atattaatga ttcattatat tattttaaac caccagtaaa taatttgata
     1381 actggatttg tgactgtagg cgatgataaa tactacttta atccaattaa tggtggagct
     1441 gcttcaattg gagagacaat aattgatgac aaaaattatt atttcaacca aagtggagtg
     1501 ttacaaacag gtgtatttag tacagaagat ggatttaaat attttgcccc agctaataca
     1561 cttgatgaaa acctagaagg agaagcaatt gattttactg gaaaattaat tattgacgaa
     1621 aatatttatt attttgatga taattataga ggagctgtag aatggaaaga attagatggt
     1681 gaaatgcact attttagccc agaaacaggt aaagctttta aaggtctaaa tcaaataggt
     1741 gattataaat actatttcaa ttctgatgga gttatgcaaa aaggatttgt tagtataaat
     1801 gataataaac actattttga tgattctggt gttatgaaag taggttacac tgaaatagat
     1861 ggcaagcatt tctactttgc tgaaaacgga gaaatgcaaa taggagtatt taatacagaa
     1921 gatggattta aatattttgc tcatcataat gaagatttag gaaatgaaga aggtgaagaa
     1981 atctcatatt ctggtatatt aaatttcaat aataaaattt actattttga tgattcattt
     2041 acagctgtag ttggatggaa agatttagag gatggttcaa agtattattt tgatgaagat
     2101 acagcagaag catatatagg tttgtcatta ataaatgatg gtcaatatta ttttaatgat
     2161 gatggaatta tgcaagttgg atttgtcact ataaatgata aagtcttcta cttctctgac
     2221 tctggaatta tagaatctgg agtacaaaac atagatgaca attatttcta tatagatgat
     2281 aatggtatag ttcaaattgg tgtatttgat acttcagatg gatataaata ttttgcacct
     2341 gctaatactg taaatgataa tatttacgga caagcagttg aatatagtgg tttagttaga
     2401 gttggggaag atgtatatta ttttggagaa acatatacaa ttgagactgg atggatatat
     2461 gatatggaaa atgaaagtga taaatattat ttcaatccag aaactaaaaa agcatgcaaa
     2521 ggtattaatt taattgatga tataaaatat tattttgatg agaagggcat aatgagaacg
     2581 ggtcttatat catttgaaaa taataattat tactttaatg agaatggtga aatgcaattt
     2641 ggttatataa atatagaaga taagatgttc tattttggtg aagatggtgt catgcagatt
     2701 ggagtattta atacaccaga tggatttaaa tactttgcac atcaaaatac tttggatgag
     2761 aattttgagg gagaatcaat aaactatact ggttggttag atttagatga aaagagatat
     2821 tattttacag atgaatatat tgcagcaact ggttcagtta ttattgatgg tgaggagtat
     2881 tattttgatc ctgatacagc tcaattagtg attagtgaat agataaaaat atgttaaata
     2941 tatcctctta tacttaaata tataaaaata aacaaaatga tacactacat aaagtgttct
     3001 atctaatatg aagatttacc aataaaaagg tggactatga tgaatgcaca gtagttcacc
     3061 tttttatatt tctaatggta acaaaatatt tttttatata aacctaggag gcgttatgaa
     3121 tatgacaata tcttttttat cagagcatat atttataaag ttagtaattt taactatatc
     3181 atttgataca ttattaggat gtttaagtgc aataaaaagt cgtaaattta attctagttt
     3241 tggaatagat ggaggaatca gaaaagtagc aatgatagca tgtatatttt ttttatcagt
     3301 agttgacatt cttacaaagt ttaacttttt atttatgtta ccacaagatt gtatcaattt
     3361 tttaagacta aaacatcttg gaatatctga atttttctct attttattta ttttatatga
     3421 aagtgtaagt atattaaaaa atatgtgctt atgtggatta ccagtaccta agagattaaa
     3481 ggaaaaaata gcaattttac tagatgcaat gacagatgaa atgaatgcta aggatgaaaa
     3541 gtaagtaatg gtagatataa taaagatatt aacaaataaa aagtgttatc caaataagaa
     3601 tagctgaaag ttatcataat tcatgaaact aataatgaaa acgagggagc agatgccaag
     3661 agacacacaa gtattaaata catataattt cgaagcaagt gttcattact atatagatga
     3721 caaggtagta tatcaaacat tggttcacaa agatggtgca tggtcagttg gtaaaatcta
     3781 ttaagctaca ttagttacag atatcacaaa ctataatagt taaacataga aatatgtgta
     3841 aattgtgatt gaaattattc aaaaacacaa aaatacgtga tgaaggacaa aatgatatag
     3901 aaaataagta tcaaacctta ataaatgatt taattgatag tttaaaagtt ataggaaaaa
     3961 tatataaaga aataaaaaca ttaaaaaaat ataagatatg tttacaaatt actatcagac
     4021 aatctcctta tctaatagaa gagtcaatta actaattgag tatctttaaa ttgaaatgtt
     4081 aggaagtgat ttaaatatga aaacttaaat tataaaaaat caatattaat ttatttttaa
     4141 aaaatagaaa ggagtgtata agatttattt tcaaagttta aaaacaagaa aatcaattta
     4201 aatttcagaa ggaataaatg tggttataga agtggattta ttatcaaaaa taataatact
     4261 aggaggtttt tatgtcttta atatctaaag aagagttaat aaaactcgca tatagcatta
     4321 gaccaagaga aaatgagtat aaaactatac taactaattt agacgaatat aataagttaa
     4381 ctacaaacaa taatgaaaat aaatatttac aattaaaaaa actaaatgaa tcaattgatg
     4441 tttttatgaa taaatataaa acttcaagca gaaatagagc actctctaat ctaaaaaaag
     4501 atatattaaa agaagtaatt cttattaaaa attccaatac aagccctgta gaaaaaaatt
     4561 tacattttgt atggataggt ggagaagtca gtgatattgc tcttgaatac ataaaacaat
     4621 gggctgatat taatgcagaa tataatatta aactgtggta tgatagtgaa gcattcttag
     4681 taaatacact aaaaaaggct atagttgaat cttctaccac tgaagcatta cagctactag
     4741 aggaagagat tcaaaatcct caatttgata atatgaaatt ttacaaaaaa aggatggaat
     4801 ttatatatga tagacaaaaa aggtttataa attattataa atctcaaatc aataaaccta
     4861 cagtacctac aatagatgat attataaagt ctcatctagt atctgaatat aatagagatg
     4921 aaactgtatt agaatcatat agaacaaatt ctttgagaaa aataaatagt aatcatggga
     4981 tagatatcag ggctaatagt ttgtttacag aacaagagtt attaaatatt tatagtcagg
     5041 agttgttaaa tcgtggaaat ttagctgcag catctgacat agtaagatta ttagccctaa
     5101 aaaattttgg cggagtatat ttagatgttg atatgcttcc aggtattcac tctgatttat
     5161 ttaaaacaat atctagacct agctctattg gactagaccg ttgggaaatg ataaaattag
     5221 aggctattat gaagtataaa aaatatataa ataattatac atcagaaaac tttgataaac
     5281 ttgatcaaca attaaaagat aattttaaac tcattataga aagtaaaagt gaaaaatctg
     5341 agatattttc taaattagaa aatttaaatg tatctgatct tgaaattaaa atagctttcg
     5401 ctttaggcag tgttataaat caagccttga tatcaaaaca aggttcatat cttactaacc
     5461 tagtaataga acaagtaaaa aatagatatc aatttttaaa ccaacacctt aacccagcca
     5521 tagagtctga taataacttc acagatacta ctaaaatttt tcatgattca ttatttaatt
     5581 cagctaccgc agaaaactct atgtttttaa caaaaatagc accatactta caagtaggtt
     5641 ttatgccaga agctcgctcc acaataagtt taagtggtcc aggagcttat gcgtcagctt
     5701 actatgattt cataaattta caagaaaata ctatagaaaa aactttaaaa gcatcagatt
     5761 taatagaatt taaattccca gaaaataatc tatctcaatt gacagaacaa gaaataaata
     5821 gtctatggag ctttgatcaa gcaagtgcaa aatatcaatt tgagaaatat gtaagagatt
     5881 atactggtgg atctctttct gaagacaatg gggtagactt taataaaaat actgccctcg
     5941 acaaaaacta tttattaaat aataaaattc catcaaacaa tgtagaagaa gctggaagta
     6001 aaaattatgt tcattatatc atacagttac aaggagatga tataagttat gaagcaacat
     6061 gcaatttatt ttctaaaaat cctaaaaata gtattattat acaacgaaat atgaatgaaa
     6121 gtgcaaaaag ctacttttta agtgatgatg gagaatctat tttagaatta aataaatata
     6181 ggatacctga aagattaaaa aataaggaaa aagtaaaagt aacctttatt ggacatggta
     6241 aagatgaatt caacacaagc gaatttgcta gattaagtgt agattcactt tccaatgaga
     6301 taagttcatt tttagatacc ataaaattag atatatcacc taaaaatgta gaagtaaact
     6361 tacttggatg taatatgttt agttatgatt ttaatgttga agaaacttat cctgggaagt
     6421 tgctattaag tattatggac aaaattactt ccactttacc tgatgtaaat aaaaattcta
     6481 ttactatagg agcaaatcaa tatgaagtaa gaattaatag tgagggaaga aaagaacttc
     6541 tggctcactc aggtaaatgg ataaataaag aagaagctat tatgagcgat ttatctagta
     6601 aagaatacat tttttttgat tctatagata ataagctaaa agcaaagtcc aagaatattc
     6661 caggattagc atcaatatca gaagatataa aaacattatt acttgatgca agtgttagtc
     6721 ctgatacaaa atttatttta aataatctta agcttaatat tgaatcttct attggtgatt
     6781 acatttatta tgaaaaatta gagcctgtta aaaatataat tcacaattct atagatgatt
     6841 taatagatga gttcaatcta cttgaaaatg tatctgatga attatatgaa ttaaaaaaat
     6901 taaataatct agatgagaag tatttaatat cttttgaaga tatctcaaaa aataattcaa
     6961 cttactctgt aagatttatt aacaaaagta atggtgagtc agtttatgta gaaacagaaa
     7021 aagaaatttt ttcaaaatat agcgaacata ttacaaaaga aataagtact ataaagaata
     7081 gtataattac agatgttaat ggtaatttat tggataatat acagttagat catacttctc
     7141 aagttaatac attaaacgca gcattcttta ttcaatcatt aatagattat agtagcaata
     7201 aagatgtact gaatgattta agtacctcag ttaaggttca actttatgct caactattta
     7261 gtacaggttt aaatactata tatgactcta tccaattagt aaatttaata tcaaatgcag
     7321 taaatgatac tataaatgta ctacctacaa taacagaggg gatacctatt gtatctacta
     7381 tattagacgg aataaactta ggtgcagcaa ttaaggaatt actagacgaa catgacccat
     7441 tactaaaaaa agaattagaa gctaaggtgg gtgttttagc aataaatatg tcattatcta
     7501 tagctgcaac tgtagcttca attgttggaa taggtgctga agttactatt ttcttattac
     7561 ctatagctgg tatatctgca ggaatacctt cattagttaa taatgaatta atattgcatg
     7621 ataaggcaac ttcagtggta aactatttta atcatttgtc tgaatctaaa aaatatggcc
     7681 ctcttaaaac agaagatgat aaaattttag ttcctattga tgatttagta atatcagaaa
     7741 tagattttaa taataattcg ataaaactag gaacatgtaa tatattagca atggaggggg
     7801 gatcaggaca cacagtgact ggtaatatag atcacttttt ctcatctcca tctataagtt
     7861 ctcatattcc ttcattatca atttattctg caataggtat agaaacagaa aatctagatt
     7921 tttcaaaaaa aataatgatg ttacctaatg ctccttcaag agtgttttgg tgggaaactg
     7981 gagcagttcc aggtttaaga tcattggaaa atgacggaac tagattactt gattcaataa
     8041 gagatttata cccaggtaaa ttttactgga gattctatgc ttttttcgat tatgcaataa
     8101 ctacattaaa accagtttat gaagacacta atattaaaat taaactagat aaagatacta
     8161 gaaacttcat aatgccaact ataactacta acga
//
LOCUS       DAGSHT010000151         8133 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGSHT010000151 DAGSHT010000000
VERSION     DAGSHT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.156"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            78..803
                     /locus_tag="MJB72_002829"
     CDS             78..803
                     /locus_tag="MJB72_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8263905.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            933..2858
                     /gene="mngA"
                     /locus_tag="MJB72_002830"
     CDS             933..2858
                     /gene="mngA"
                     /locus_tag="MJB72_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBY8263906.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            3002..5617
                     /gene="mngB"
                     /locus_tag="MJB72_002831"
     CDS             3002..5617
                     /gene="mngB"
                     /locus_tag="MJB72_002831"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBY8263907.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSTQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            5877..7031
                     /locus_tag="MJB72_002832"
     CDS             5877..7031
                     /locus_tag="MJB72_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBY8263908.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(7154..>8133)
                     /locus_tag="MJB72_002833"
     CDS             complement(7154..>8133)
                     /locus_tag="MJB72_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263909.1"
                     /translation="FVVKFTLHVKNLISRAKNNQVLRNQIPQKLKDSYPLIYDISVYI
                     CNQIQILENIEIDEDEISYISLHVGSFFDRQKLLEDKILCALITPNYYDLQFKIVRDL
                     EKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPISDRCPIPFVYVNPYLNRKDYDNI
                     QSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLDSAKDYIRFMGNILYENKYVKPN
                     YIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVCLIVNDKPVKWGEEKVQIIAMIP
                     INEKEKEKFNYIFESFIEILSEWNNVKELTKADNYSSFMNRIAYLIQNI"
BASE COUNT         3266 a          904 c         1475 g         2488 t
ORIGIN      
        1 ataattatat taacgaaaat tttagctaat atttttgaat tttacttatg aataataaaa
       61 tagatggagg agaaactatg gaattttatg aaagagcaaa aaaatatgag tataaattta
      121 cagatacaga agacatgata attgaatata tgagaaaaaa caaagaagaa atagtaaaat
      181 tatcaataca atctttagca gataaatttt ttactgtccc aaataccata gttaggctat
      241 gtaaaaaaat ggagtatgat ggtttctctc agctaaaaaa taatttaaaa gaagagttaa
      301 aaaatgagga taaagagcca aaaaatggtt ttgaatcaga tataaaaaag acattagagc
      361 taatagatta tgaaaaaata aataaagtag cagatataat tcaaaaatct aggagtatta
      421 tagtttatgg aataggtcaa aacattcctt tatgtgagat attagtaaaa gaaattagag
      481 gtgggcatga taatataact tactatagtc aaagacatga agtacttcat gatataggaa
      541 aattaggtga acaagatatg tttttcgcac ttagtttaag tggagaaacg aaagaagtta
      601 tagaagcagc aaagacagca gttaataagg gttgtaaggt agtagctcta acacatatat
      661 gtgaaaatca attgcaacaa attgcagata taagtttata ttgctattca acaattaaac
      721 aagtaaatgg gtacaatata tcagataagg ttccaattat gatgattatg agagcaataa
      781 tagagcaata ctggttaaga tagagatttg tgtataaata cacaaatctt tattttttaa
      841 gaagaaattg tgtgtataat ttcaaaaaaa cattttcata atttatttta tgctatattc
      901 atagcattaa atgatttaag gagggcgtaa ttttggaatt taagaaaatc acaaatgaaa
      961 accttgtttt tttaaaacaa aaaataaaaa caaaggaaga cgcaataaag ttcttagcaa
     1021 gaaagctata tgatgaaggc aagttaacat cttatgagga atatttagaa gctgtaatgt
     1081 acagagaaac tttatctcca acaggttttg aaagagggct tgcaattcct catggaaaat
     1141 ctaagtcagt taaagaagct gcatttgcag ctatgacctt agagacacca atagaaaatt
     1201 gggaaagcgt agacccaaat aataaagtag agataatatt tttaatagca gtaccagaaa
     1261 atgaaggtgg aaatacacac atacaaatac tttctgagtt agttactaga ctgtcaaatg
     1321 aagattatat gaatatgttg ttaaattcaa aaactaagaa agagttattt aataacttag
     1381 atataaaaga aaagaaagaa caaataaaag aagagattag gattcaaaat gaatcaaaaa
     1441 aaattatatt agcaataaca gcatgccctg caggtatagc acatacatat atgtcagcag
     1501 aagcattaat taaagctggt aaagaaatcg gtgtggatgt gtatgtagaa aagcaaggag
     1561 ctaatggtat ggtggaccct catacaccag atataataaa aagggcagat gcaataatat
     1621 atgcaacaga tgtagcacct aaaaatacag aaagatttga acatttgcca aacataaaaa
     1681 cgtcagtagc agctccacta aaaagagcaa aagaaataat atacgaagct ttagaagtag
     1741 cacaaaaaca aggtaaaggt gattatatag aaaaatcatc tgatatatca tattgtgaaa
     1801 aatcatcatg gaaaaaagat gtaaaatcag caatacttac aggagtatct catataatac
     1861 cacttattgt ggcaggaggt atgatacttg catttgcagt attagcatct caagcatttg
     1921 gtctacaaga actatacaat caagaaggtt cttggttatg gcaattaaga aagctttctg
     1981 gaggtatgtt aggtacattg atggttccag tattatcagc atatatgtct tactcactag
     2041 ctgaaaaacc aggtcttgga ccaggatttg cagcaggttt agcagctgat ttaataggta
     2101 gtggatttct tggaggtatg ttaggaggtt tacttgctgg ttatatagta aaatttttaa
     2161 agaagaatat accagccaaa ggcacattag ctggattcgt aagtttttgg gtatatccag
     2221 tagttggttc tttggtagta ggtttattga tgttatttgt agtaggaaag cctgtagcag
     2281 cacttaacaa tggtcttata aattggttaa atgggatgac tggagtaaat gcaatactac
     2341 taggtgcaat aataggagct atggtttcat ttgacttagg tggaccaata aataaagcag
     2401 cctatacatt tgcaatagga gcaatagcaa gtggaaattt tataccttat gcaatatttg
     2461 catcagttaa gatggtttca gcttttggag ttacaatagc tactgtttta ggcagagatt
     2521 tattttcaga atcagaaaag gaaataggaa agcaaacttg gatattagga ttggctggaa
     2581 taactgaagg tgcaatacca tttatgatag aggacccaat ccgtgttata ccatcattgg
     2641 tagctggttc agccataact gggtctatag tagcattctt caatataggt cttcaagtgc
     2701 ctggagcggg gatattctca ttggcgcttt tagagggaag tatgtcagga tttattatag
     2761 catcaatatg gttctttgcg gcagtacttg gagctataat atcagcaata ttattaataa
     2821 taactaggaa acaaaaaaat caaagaaaga tacattaaaa gatacgttat tagatgttta
     2881 aatcaaaggt ttaattatag tgataaaata aatattaaaa aattttgtaa actcagtagt
     2941 atttttattg gatttttaat tagacagaaa atattatcag ataagttttg gaggataagc
     3001 aatgaaaaaa aatgtacaca tagttcctca tatgcattgg gatagagaat ggtacttctc
     3061 aactgaggaa tctagaatat tacttgtaaa taatatggaa gaaataatgg atatgttaga
     3121 aaataatcat aactatccat attatgtgat ggatggtcaa actgctatat tagaagatta
     3181 tttagcagtt aagcctgaat gtaaggagag aataaaaaaa ttagttcaag aaggaaggct
     3241 aataataggt ccttggtata ctcaaactga tgaaatggta gttggtggag aatccatact
     3301 tagaaattta ctatatggaa taaaagattg tgatgaattt ggagaatata tgaaaatagg
     3361 atatttacca gattcatttg gtcaaagtgc tcaaatgcca caaatactaa atgggtttga
     3421 tataaaatac tcaatgtttt ggagaggatg ttctgaaaga aaaggaacta ataaaacaga
     3481 gtttaattgg aagagtgatg atgggtcttc tgttttggtt caaatacttc cattaggtta
     3541 tgcaatagga aaatatctac caatgaatga agatgagcta agaacaagaa tggataaata
     3601 tttaccggtt cttgataagg gtgcaactac tgaccatata atacttccaa atggacatga
     3661 ccaaatgcca attcaaaaga atatatttga agttatatat aaattaaaag aatgttatcc
     3721 agagagaaaa tttttcttaa gtagatatga aaacatattt aaagagctag aaaaaaatga
     3781 ggatatagat acaataaaag gggaattttt agatgggaaa tatatgaggg ttcatagaag
     3841 tatattctca acaagaatgg atataaaatc agcaaatgct agaatagaat caaagataac
     3901 taacatattg gaaccactag cttctattgc atattctcta ggatttgaat atcatcatgg
     3961 attaatagag ttaatttgga aagaaataat gaaaaatcat gctcatgaca gcattggatg
     4021 ttgttgttca gataaagttc accatgaaat aatgaataga tttttcttag ctgaggaaaa
     4081 agtagaccaa ttaataacat tttacaaaag aagaatagtt gatgcaatga gttgtgaaat
     4141 ggctttagat aagttagtag catttaactt aatgccttac gataggaatg aaatagtgag
     4201 cacccaagtt ataactaaaa tgaaagcatt tgaaatattt gataaagata ataataagtt
     4261 ggattttgaa gtggtacata aagaagtaat agatgcagga ttaatagacc gtcaaattgt
     4321 tcattatgga aactatgatt catttatggt atatacaata aactttaaag ataaaatacc
     4381 tgcaatgggg tataaggcgt atatgataaa agaagttgaa catatgttag aaaaagctta
     4441 tgaagtatgt gacatggtag ataatgactt ttatactatt caagttaatg aaaacgggac
     4501 attaaagata tttgataaaa aattaaataa aacttttgaa aatgtattat tgatggaaaa
     4561 tggaggagat gaaggagatg agtatgattt ttcaccactt ccaaatgaga aattaatatt
     4621 taacgataat atagtagcaa attccactat aaagaaaaat agatttaata atgagataaa
     4681 gataaattat agattaaaag taccaaaaga tatacaagct agaaaaaata atagagcaga
     4741 ttcatttatt gactttgata tagttataaa tgttccaaat gataaagcta taatagatgt
     4801 agtattcaac ataaataacc aagcaagaga ttgtagggtt agaacttata taccaacaaa
     4861 tatagcttca aagttttctg tgtctgataa tcagtttgga catataaaga gaagtgtgta
     4921 tgatgatgct atggaagttt ggcaacagga agaatggagt gaaagaccag attctatata
     4981 tccaatgcta acattcgttg gattgtctga caaagagcat ggagtagctg ttcttacaaa
     5041 cagtacaaga gagtttgaaa tagtagggga aaaatttgac acaatagcta taacattatt
     5101 tagaagtgta ggattcctag gtaaagaaga aatggcaaga cgcccaggtc gtccatcagg
     5161 aataaagttg cctactccag attctcaaat gataggagat ataaaaatag acttagcaat
     5221 agttactcat gaaaaatcta cactagaagc aaatgttgca aatatggcta aagagtattt
     5281 aacaaaaata caaacttata ataaaatgcc atataatgct atgaaattga atcaatcaga
     5341 aatagaagta gattattcat atagtttact taaggaaact aataaggacc ttgtgttaag
     5401 tgttgtaaaa aaggctgaaa aagaagaggc attagtagtt agattttaca attctaaaga
     5461 agaaacaaaa tcagctaaca tagtatttaa caaaaatatt aaaacagcta aatggattaa
     5521 cctaaatgaa aaagaattag aatctatatc aatagataat aactctgtaa atgtagagtg
     5581 cttaatgaac caagttaaaa ctatattggt taaatagtgc taaaaaggaa ttgtcttaaa
     5641 atagagataa tcctccttat cattgttgaa ataataaaca tatatttgat tttatacaaa
     5701 aagagtgtct catgaactaa gaaatttatt ttgagacact ccctttttat aagataaaag
     5761 aatattaatg gtataagttg tatacaaaga gagatgtaga gtataaaata aaagataaat
     5821 taagcttata aaattatatg tttaaaataa ggagtaataa aaaggagtag ataagtatga
     5881 aaatattaat ttctatagat tctttgaaag gaagcttatc ttcaatagag gcaggaaatg
     5941 caataaaaaa ggggatattg aaggttaaag aagatgcgca agtaaagata ttgccacttg
     6001 ctgatggtgg tgaagggaca gtagatgctt tagttcaagg gatgaatggt aaaaaagaga
     6061 ctatagaagt tactggacca atagctaaaa aagtggatgc aacatatgga ttattaaaaa
     6121 atacaagtac agcaataata gaaatagctc aagcatcagg actaacattg gttccgactg
     6181 aacttagaaa tcctttatac actacaactt atggtgttgg ggaaataata aaagaagcaa
     6241 taaataaagg ctatagaaat tttatagttg gaataggtgg aagtgctact aatgatgctg
     6301 ggataggtat gcttcaagcc ttagggtttg agttttatga tgaaaataac aagttagtag
     6361 gattaggtgg aaaagtttta aatgaaataa gacatataaa aatagaaaat agattaaagg
     6421 aattagatga atgtaagttt aaaatagcct gtgatgtaaa taatccgctg tttgggaaaa
     6481 atggagctgc atacatatac gggtctcaaa aaggtgcaac atcagaaata attgagaagt
     6541 tagataatgg gcttagaaat ttttccaaag tagttaaaaa ttacttaagt aaagatgtag
     6601 ctaatgtaga aggagcagga gcagcaggag gattaggttt tgctttttta gcatttttaa
     6661 attcaaaatt agaatctgga ataaaaataa tattagaaga aattaaatta gaggaagagt
     6721 taaaagatgc agattttgtc ataactggtg aaggtagatt ggacaatcaa actgctatgg
     6781 gcaaggctcc aataggagtt gcaaaactgg caaaaaaata tggggtaaaa gtaataggtt
     6841 tggctggagc cacaacagaa gatgcagtta aatgtaatga agagggtata gatgcttatt
     6901 tttctatagt aaatagagct atgacaatag aagaagcaat ggataaagca actgctagtg
     6961 agaatatgac agctacaaca acacaaatat tcaatcttat aactagtata caacagtcaa
     7021 aaaatatgta gtattaaaac aaggtatatt aggtaaaaag ctgtcacata ttaatttaga
     7081 ctttttagtg tgacagctta tctattttat gttaataact taagaaacat atattggaaa
     7141 taaattaatg gatttagata ttttgaataa gataagctat tctattcata aaactactat
     7201 agttatctgc cttagtaagt tctttaacat tattccactc agataatatt tcaataaagc
     7261 tttcaaatat gtaattgaat ttttcttttt ctttttcatt tataggtatc atagctataa
     7321 tctgaacttt ttcctcaccc catttaactg gtttatcgtt aacaattaaa catactccag
     7381 tttttaaagc atccattttc atgctatgag gaattgctac attgttatta aatgcagttg
     7441 aagacatttt ttctcttata agaacatcat ctatataatt gggtttgaca tatttatttt
     7501 cgtacagaat atttcccata aatcttatat aatctttagc actatctaaa tatatatttt
     7561 tcataaacaa agattcacta aagtacattt ctaaatgatt ttgtacagta agtatatttt
     7621 ttctatcttt aatctgaata aatttacttt gaatattatc ataatccttt ctatttaggt
     7681 aaggatttac ataaacaaaa ggtatagggc atctatcgct aattggtaaa gtagtaataa
     7741 ccatgtctac cttattgtca aaatcaagat tatgagtatc tgaaaaaatc tgaataattt
     7801 ctatggattc attaaatctc ttctcaaggt ctcttactat tttgaattgt aaatcatagt
     7861 aatttggagt aattaatgca cagagaattt tatcttctag taatttttgt ctatcaaaaa
     7921 atgaacctac atgaagagat atatacgata tttcatcttc atctatttct atgttttcta
     7981 aaatttgaat ctgattacaa atataaacag atatatcata tatcagaggg taggaatctt
     8041 ttaacttctg aggtatttga tttctcaaaa cttgattatt tttagcccta ctgattaaat
     8101 tttttacatg aagagtaaat tttacaacaa att
//
LOCUS       DAGSHT010000152         8064 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.157, whole genome shotgun sequence.
ACCESSION   DAGSHT010000152 DAGSHT010000000
VERSION     DAGSHT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.157"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(9..230)
                     /locus_tag="MJB72_002834"
     CDS             complement(9..230)
                     /locus_tag="MJB72_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263910.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(731..1711)
                     /locus_tag="MJB72_002835"
     CDS             complement(731..1711)
                     /locus_tag="MJB72_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263911.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1875..2747)
                     /locus_tag="MJB72_002836"
     CDS             complement(1875..2747)
                     /locus_tag="MJB72_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8263912.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(2911..3462)
                     /locus_tag="MJB72_002837"
     CDS             complement(2911..3462)
                     /locus_tag="MJB72_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8263913.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3482..4384)
                     /locus_tag="MJB72_002838"
     CDS             complement(3482..4384)
                     /locus_tag="MJB72_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBY8263914.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4399..6771)
                     /locus_tag="MJB72_002839"
     CDS             complement(4399..6771)
                     /locus_tag="MJB72_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBY8263915.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(6992..7681)
                     /locus_tag="MJB72_002840"
     CDS             complement(6992..7681)
                     /locus_tag="MJB72_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY8263916.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7922..>8064)
                     /locus_tag="MJB72_002841"
     CDS             complement(7922..>8064)
                     /locus_tag="MJB72_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY8263917.1"
                     /translation="VHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLDQDTDSW
                     GD"
BASE COUNT         2584 a         1459 c          767 g         3254 t
ORIGIN      
        1 atattatatt aaattttatt attacatttt ccacaacaat actcatatgt ctccacatta
       61 tttggaatat ttacaaatac aaattcttta cagttctcac aatactttaa tatagatata
      121 gttccacaag tgtaacattc aaatattaca caatcctcat tttcctctac tatttcaaac
      181 tttttactat gttcaccaca ctctgggcac ttgtgattat ttacattcat aaatactcct
      241 ccttattagt atagttacat gtcaaaaact taattaatgt attctatatt acttattata
      301 tattctataa taaatcatat tctaatataa taaaacaaat taaataaaaa aaaaccataa
      361 attttaaatt tatggattaa ttcttgtaat gataactcta tttgaggatg tgagggaggg
      421 ttatcattac aatagggtat tatattatgg tttttaagct agtctttcta cacctcatat
      481 gatacatatg ctatatgaat tttatatgat ttttacataa atattttatg aaatttaaat
      541 tttttagtca ttttgaaatt tgtaaaaagt tctctaaaaa tccacagcat cataaacagc
      601 caaaatttaa gtagaacatc ataaaaaaat ttgtccaata attttaacaa gtaatctcac
      661 ctcattataa ttattgacac aaacttaaat aaacaatcta taaatcttct ataaatctta
      721 aactttcccc ctattgatac acaatttcta gtttgtcaat tttattttgc atttctaaga
      781 aaatttcttc aacagaattg ttaaatatat tgcctaaata gattctttta tctttttgaa
      841 atgaataaga ttttacacta ccatcagaag aaatatataa atgactccac tctgctccat
      901 attttgaaaa cctctccttt gtatctttaa acctcatatc ttctattttt attgcttttt
      961 ctctgtctcc tcttactaca gtgtgacccc tttttattaa actggttatt tttatatctt
     1021 cttttaattt aatacgtgta tcatcatttt ttgaatatac tttccatatc actttatgac
     1081 gataattatc taattccttt attaagcttc tatcaatagt aaaactatct ttagttacca
     1141 caattaaatc aaaatggtct aacatatatt gaaatatttc tctcatactt ttatttttta
     1201 aaatatcctc accaataacc tctataaatc tacagccttt ctcatacatg cagttcaata
     1261 aacttgtgat ttcagaaaaa taatcatttg tattattatc tccatctatt tctatagtaa
     1321 agtacaaagg agtaatgtat gatttatttc ctgttatctc aaatacactt gatagtgtct
     1381 gtatatcatc ttcaaacgtt aagtatacat tctcataaaa cataaaaaat tccataactt
     1441 tttctttcat attatggata tttctatatc tattggataa tatatttatt atatcgtcag
     1501 tagtattttt cccatcacat tcagccagta taacaatttg cttccaactt aagctatcca
     1561 catttccgat acttttttta tatccatctt cttcatcata aataggacca attataatgt
     1621 tttcttttgg ccacagatga accctagttc catcttttaa tttcggatat ttatttttta
     1681 cagactcata aaatctactt gtatcaccca tgattcttta ctacccccca tagtttaaat
     1741 attctatgct aaaaatagca ctttattttt attaattcaa caattattca aaattggtca
     1801 ttatatatgt ttaaatggat ttaacatcaa ccttatttat atgttattga tgtttatata
     1861 tgttatataa aatcttattt ctcatacttc aatgtaatct taactatctc tggtttacaa
     1921 ttaagcctaa ttaaaacttt tgagcctcct aagcctttat ttaaaatcat agtgctatta
     1981 tctttttcat attttcccca gtcatactta ggaaagaact ctctatttgg agataataca
     2041 cctccaacaa aaggtattct tattatacct ccatgaacat gtcctgctaa aaccaaatct
     2101 gctccccatt ttgcatattt ttcaaagaaa aatggtgtat gtgctaaaag aatgttatat
     2161 tcattttcat taactttacc tagattgtct tctacaaaat tgtcacttag ttctaatttt
     2221 ttgctattag caaataaata tggatagcat tccaatggta taattaaacc atataaatta
     2281 agatggctat tacctctata tattcttaca gattcatttt ccatatttat tattttcttt
     2341 gaatatagtt ccttaaaata atccttatat agatgtttat acttttttat taaagatttt
     2401 tgttcatgat ttccaattat atgatatact tcatattttt caaccaaatt atctattaaa
     2461 tctaatgcaa cttgaaaatt tttattttct ccatctacta aatctcctgt tataaatata
     2521 acatctgggt ttaatgattc aattttatct atcaatacct tattattttt atcaaacacc
     2581 ttattatgta aatcactcac ttgaactatt ttaaagttat caaattcctt tggaattttt
     2641 ttgttgaaaa tagtatattc tgtcgtttct atatgctgag tttcatactt atacagaata
     2701 tacaataata ttaatactaa tactgtcatt gaaaccttta taatcatatt ctcttcctct
     2761 ttattctatt ttgttaatat tattataatg cttttatgtt ttttttaaaa caataacatc
     2821 atcacataca tataaatctt ttcgacatta taaaaataag ttatcctttt ctaaaatttt
     2881 catttatttt agaaaaggat aactataaaa ttatctataa acctctatat tgtgaaatat
     2941 agtttttcct ttatttaaat ttttatatat atgctctaaa gttccatcaa acctaataga
     3001 atgtccagac tctaaaatat aagttttccc atccacatta aggtcaagtt taccttctat
     3061 tacgagtaca tactcttcta aaacatgact gtgtgtagat gaaacatgag tacagttttc
     3121 ctcaagctca attataaaaa gttcaaacct tctttgaggg ctaaaagggt atattggata
     3181 taacttcatg ttcccttttt gctcaacaat aggctctact tcttctttct ttataacctc
     3241 ataggtattt gtattttcat ttaatagtgt agtaaaattt attttaaatc cagatgaaat
     3301 tttccataaa gtagatactg tcggacttga ttcacctctc tcaatctgtc caagcattgc
     3361 tttactgaca cctgtcatct ttgaagcttt gtctaaactt atgcccattt cttgtctcaa
     3421 taaccttaaa ttttctccca cattaggaaa tttattatcc atgttaaaat cctcctgata
     3481 gctatttgta ttctatgatt tttactcctg taagttctac tatctctttt aattttaaca
     3541 tatgattgtt acaaatttca tcaaattcat atatataatc ttcaagccct aatgccttat
     3601 atttatcgat tccaaattta tgatatggaa gtaactccat cttgcctcct ggaacatttt
     3661 gtttgacaaa taatgcagta tttcttatat tttcttcact atcatttact ccataaataa
     3721 ttggcactct tataaccata gatttattca actttgaaag cctacatata ttatctaata
     3781 taattttatt acttactcct gtatattctt tatgaatatt atcatccata gattttatat
     3841 ctacaaatat gtggtctatt ttctcaaata catcatttac cttattccaa tcaaaatatc
     3901 cacaagtttc aattgcaata tttatgcctt tatcataaaa aatatcaact aattctctta
     3961 aaaaatcaat ctgaagtgta ggttcacctc cagaaaaagt tatccctcca ttagattcaa
     4021 agaaaaatat aaaatctttt tcaacttcct ctactatttc ttcaacactc atttcttcgg
     4081 tcattataca aatggcatct ttaagacata ccttgataca ctctccacat aaatcacatt
     4141 tattatttat ttgcgctcta tcaaatagtg aacttatatt ttgaggacaa acatctatac
     4201 atctattaca ggatacacat ttatctttca taacacctaa ttttactatg ttactccagg
     4261 aatctggatt agaacaccac ttacatttta atttacatcc ttctaaaaat atagtagtcc
     4321 ttattccatc tccatcattt actgaaaaat gttgtacttt tacaactcta ccttttttac
     4381 tcataatcta cctcataatt aaaaattgct atgtgaattt ctagctatta tagcatcttg
     4441 catttctctt gataaattaa caaattgagt actatatcca gcaactctca ctaataaatc
     4501 tttatattct tctggatttt cttgagcttt taatagagta tcatttgata tcgtattaaa
     4561 ttgtacatgg aatcctccta aagcaaaata tgatttaatc atagatgtta gatttctaag
     4621 tcctctttca gtttccacca aatcttgatt cagtcttaaa tttagaagtg ttccaccagt
     4681 atgaacatca tgatttattt ttgatgcaga tttcattgct gctagtggac ttgttgtatc
     4741 agtaccaaca aatggagaaa ctccctctgt caaaggtttt gtcgcttttc ttccacaagg
     4801 agttgcgcct acgatttttc cagttggtac atagtttgat atacccataa atgcactatt
     4861 aaatttagag ccaaaaatat ccttgtattt tctagtttca gtataataaa aatcagaaac
     4921 ttcaattgct aatgaatcta cataatcatt atcatttcca tacttaggaa catccaacgc
     4981 aagttttctt aattcttcat aaccttccca atcattattt agtgcttttg ttaattcttc
     5041 taacgtagtc accttatcct caaatactaa tttttttata gctgccaatg aatttgaaac
     5101 tacacctaac cctataccag ttaatacagg acctatatta tattttgctc ctcctttact
     5161 taaatcaact cccttatcca aacatccatc tacacaagta gataaaaatg gtcttggtac
     5221 catttcttta tgaatttgtt gagcaacagt agttcctata acagaacttt ttacaagata
     5281 tgctaattgc tttaaaaact catccttaag ttcttcaaaa gaagtaaatt ctgtgatatt
     5341 ttttgtatct aaccccattt tttctccagt taatctactc ttaccttcat tcatagcata
     5401 ttctaaagct gctgcaaaat tgatatttac ggctgaagtc cactgtcctg tttttctaaa
     5461 atgaggaact acacaaccac agttactcca atctctagca tcttctgcat catatccatc
     5521 ttgtaataac atttgtgccc ctgcactatc gctgtgtata gcagggaaac cagttccttg
     5581 ttttaccaat ttagcaactg ccattacgaa attgtctggt gctccttgac tcactctaac
     5641 acttaatcct ggttggtgag tttttacact ttctgtagcc tttaaacaca tatatgagat
     5701 atcattcgtt ccatcagtac catttctctt tttaccacca acagtaagat tttggaattg
     5761 attatatcct gcaaagtaat ttgctgtatt tgctgatata gtccataccc attctgataa
     5821 cttaatccaa agtgcttcta tcaattcttg agcttctgat tcagttattt taccttctct
     5881 agcatcattt tcataatatg gatacatata ttgatcaaat ctacctaaat ttaatgccaa
     5941 tggattttct gatagaatac cacctaattg aacaaaccat acaaattgta ttgcttcata
     6001 aaaattagtt ggagggtttg ctggtacatt catacatatc tctgaaattt ttataagttc
     6061 taactttctc ttttcatctg tttcttgctt actcatctct tttgcaagat ttgaatatct
     6121 ttgtgccaat gtcataatac cctgtgcaac tatagaaaca gatttgtaaa aatctatctt
     6181 ttcaatattt tctgaaactg atatatctaa ttctttaatt tttttatctg catcttcctt
     6241 aatcccttta tatcctttta caaacaatat atcttgataa tctggtgtta cttcaccaac
     6301 tgcctgtctc cacttagaat cattatctat tattcctgta tctactaata tcttagctgt
     6361 atcttctggg attcttgcta agaaaacttc ttccaaagat tttcccttcc aatatggaaa
     6421 tatttctttt cttataaatt ctatttgttc ttttgatatt acatatgggt cttgagttct
     6481 tttatcaaaa gtatccattt ctttgtctac ccattgccaa gaaaactctg gtgtcaatat
     6541 ccctgttctt ctaaattttc ctgctgtacc tactattaat tcattttcaa agatttttat
     6601 aggaagttct ttacatgtct ccaaaaaagc tttagctctt cttatacaga tagcttctcc
     6661 ctcagttttt ttatgtgatt ctgtaaaaat tctagccctt tcacaacata tcataggttt
     6721 agaattaata tattcttgtc taaatgcttc tattctatta gtttgactca tttaaaatcc
     6781 ccctttggat aatatatact acatttttgt atgttataac atactttaat tatattatac
     6841 tgtcctttag cataaaatgg aagcaaaata acttattttt tttcatctac cttgaacacc
     6901 tatatattaa tacttttttt gttaaattta taaaatctaa aaaaattttc tatgatatat
     6961 tttctatatt gttatagtta tgtctaatat actaatgatt tgaaatatca aatttgtata
     7021 ctagacttag ttttaaaacc ttagttgatt gtttaacttt gttaacttca acatgtatat
     7081 ccttcatata aggaagctct acaacaccta ctcccttatt aaagtcttct tcaaccatag
     7141 aaaaaatttc tttctcagtt aagttcgtat cagtcaatga tttaaataaa ttgtatataa
     7201 atttaacata tttatcactc tcagataact tatcatcttc tggatactct ttagcacaga
     7261 gaatatctat tgttactttt ccttctttat cgtcttcaga catagaatag aataaacttc
     7321 gaatattttt ttcaggtggt ttttcagcac catttataga tactacaaag ctaccatctg
     7381 attttgagtt agtaatacta taaccactgt ctattatagc tttcgaaaat ctttgagata
     7441 aatctttttt tacttcttct ctacctttcg catcattaat cttttgtgag taattattaa
     7501 atagctcaat agattcatct tttttatctt caacttgttc tttcaatgaa tctttactaa
     7561 tttgttctga gctacttaag ttcttatcag atacaggaga catacaccct accatcaatg
     7621 ctaagctaag atttaaagcc acaattattg ccactctatt tatattcctt tttactttca
     7681 tacacaatcc tcccatcaca ctaaccgctc taaagtatta ttcattgcac tagaatatat
     7741 ttatattttc tttatattaa agatttatta ttcaatacat atcaccttcc tatccaattc
     7801 ttataactta ctattagtaa aattataagc tccttaatac agaaataagg atgttatctt
     7861 ttgttattaa aagataacat ccttattttt ttgtatcaat acatatgtcc caactggtaa
     7921 cttaatcgcc ccaactgtca gtatcttggt ccaatggtat atatatattt agtataaatt
     7981 tatcgatact attttcaaat aataattctc cattatattt ttgtaaagaa gatttaatac
     8041 tctgaatacc tattccatgt acga
//
LOCUS       DAGSHT010000153         7925 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGSHT010000153 DAGSHT010000000
VERSION     DAGSHT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.158"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..545)
                     /locus_tag="MJB72_002842"
     CDS             complement(<1..545)
                     /locus_tag="MJB72_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY8263918.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTR"
     gene            complement(902..1600)
                     /locus_tag="MJB72_002843"
     CDS             complement(902..1600)
                     /locus_tag="MJB72_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8263919.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(1634..4336)
                     /locus_tag="MJB72_002844"
     CDS             complement(1634..4336)
                     /locus_tag="MJB72_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBY8263920.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKDIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(4514..6331)
                     /gene="ftsH"
                     /locus_tag="MJB72_002845"
     CDS             complement(4514..6331)
                     /gene="ftsH"
                     /locus_tag="MJB72_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBY8263921.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFEESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(6362..6805)
                     /locus_tag="MJB72_002846"
     CDS             complement(6362..6805)
                     /locus_tag="MJB72_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8263922.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(7296..>7925)
                     /locus_tag="MJB72_002847"
     CDS             complement(7296..>7925)
                     /locus_tag="MJB72_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBY8263923.1"
                     /translation="YVKEFYKTFDEAKGEYFDGFIITGAPVEQMEFEEVDYWNELEEI
                     MDYSKRKTKSTLYICWAAQASLYKYYNVKKLPLSQKCFGVFKHKVDKDSKIVDGFENE
                     FFAPHSRHTTVNIEALKENKELSIVSHSKEAGPYIITNSRDVFVMGHSEYDKYTLDKE
                     YKRDINKGDKISIPQNYYINDDPSEEPTVKWKKHSELLFRNWIKNYLIQ"
BASE COUNT         2514 a         1411 c          842 g         3158 t
ORIGIN      
        1 attcttgtta atcctcttac atcacttatt ctagatacat gattcttatt aatgttgtcc
       61 atcatttcat cttctatttg aagaattaac tcttcaactt tttccaaaga ctcaaaacca
      121 ttttttataa cttctctaag aatcaagtag tttatcttaa atagattaat aactggattt
      181 ttttcaagtt gagagttctt caaaataata ttttttacaa attcaaataa aaagtgttct
      241 tcattcacta caaccaatat aaaattatct gataaataca tatttacctc ttctataaca
      301 gcttctccat ctcttagttc aaatgtattt aggcttaaaa aatcgtaatt atcaaataaa
      361 tcaagtttgg ttatttcatc aaatttaaga caatcattaa aagttatttc atctatatcc
      421 aaagtttcct taagcaattt taaatttttt ggagcactta atataagata agaattttct
      481 gctacataaa atttatctct aaaatcttca atagtctctc ttgtatttaa attcagtata
      541 tacatacatc tactcctaaa tttcaattta cattctaaaa attttttata atccttactg
      601 taattatttt acactatttt aataattatt atttaatatt tatattaaat ttattataag
      661 gtaagtgtta aatacattta ttatttaacc aaagttttta ttacagatta cactatatac
      721 ttattttaaa acatatttca tattattgat actaaaaaat tattgatact aaaaaagcta
      781 aaaaataaaa tgcatgtgct aaactgacaa caagcacata cacttcattc ttattttaac
      841 taaactatat aattttaata tatattaata agcacataat ctaataatta atatattaca
      901 actattcatc atttaatctg tatcctacac caacttctgt aattatatat cttgggtttg
      961 caggttgttt ttctattttt cgtcttaacg ttgccataaa gacccttaaa gacttagtct
     1021 cattccctat aacactaccc catatttctt ttattataaa attatgagta agcacttttc
     1081 catgatgctt tgcaagtaat gaaagaatat tatattcaat aggtgtcaaa tggacttcaa
     1141 catcatcaac aataactttt cttttatcaa aatcaatcaa taatttatct acttcaaacg
     1201 tagatttaac atcatctttt tgttgagctt ctacttgtga atgtctaaat gctactctta
     1261 ctcttgctaa taactctact atggagaatg gttttgttaa gtaatcatct gcccctgcat
     1321 caaatacttc taccttgtct ctatcttgct ctctagctga aactacaatt attggtatat
     1381 ttgaaaattg tctgatagct tttattacct ctattccatc catatcctct aagcctaaat
     1441 ctaaaagaac aacatctgga gaatatgaga cagctataga tataccttct tttccagtta
     1501 tagcctcctt gacatcataa tcctgtgtta aaagtgcagt agatataaat tttcttattg
     1561 tcttatcatc ctctactaac aatataagtt ttttactcat ttcttcctcc aaaactaatt
     1621 ttaaaattta aaactattca ttttccttag gcaaactaaa cttaaaagtt gaacccttat
     1681 ctttattgtt ttctaccata atttcacctt tatgagcttg aattatagat ttacaaattg
     1741 ataatccaag tccaacaccc tttctggaat ctttagctcc ttcttctcca gtaaaaaatc
     1801 tgtcaaagat atgcttttta agttcctttg atataccagg accattatca gatacttcaa
     1861 accaaacata atcatctttc tcatatacat ttatctctat cttacaatct tcctttgaat
     1921 atttcaaaga attatctatt aaattaatta aaacttgttc tattaatttt gcatccatag
     1981 gaacaaataa tatctgttct ggtatattaa tatctataag tgcattttca attctttttt
     2041 ttattttttg taacgcttct gatataattt cctcaaccag ttcagaatgt tttttaactt
     2101 ccaatttccc ttcatctatc ttggtcatac taagtaaatt ttcaacaagt cttattaacc
     2161 actgagtatc ttcatataca ccactaagta attcatctat tatatcttga cctatagtac
     2221 ccttgttttt tataattgta cttactgcac cagatattcc tgctagtgga gttctaaggt
     2281 catgcgaaac tgctcttaaa agattacttc ttaatctttc cctttcaatc tcaagattgg
     2341 tattttcttt agcttctgac aagatttctc tatcaagcgc tattgccatt tgagcaatta
     2401 ttgtttcaat taagcttata tcttctgtat ccaattttga atcaatacat gatattccca
     2461 ctactcctaa agttaaattc attcctacaa ttggtatata gtatcccttt gaaccaggaa
     2521 gtgtatccgt gtcatttcct gccactgttg aattggataa aacccaactt gcaacagctt
     2581 tttcatcttc actatccaaa tcatttttat ccttattatt agtatttttt ttgtaaagct
     2641 ttgatttatt atgcttatct acatttaaat aacaaattac atctctactt aagctaagag
     2701 acaaaagttc tatacctttg tttactatat catctttatt tgataatctt aaaaatccct
     2761 taattatttg atatatcatt tttgtatttt cttctctttt agcagcagtt tctgcttcat
     2821 gctgtatctt agacgtaaga gtgctagtta ttatacctac tattgagaat acaaccagtg
     2881 ttactatata gtttgaatct gctatagata atgtatataa tggtgctgta aaaaaataat
     2941 ttaaaagaac tatattaaat acagaactta ttatacctgt actataccct ttagtccaca
     3001 tagaaactaa aactacaccc aacatatata ttaacaatat attttctctt ataaatccca
     3061 tgctttgtac tgcatatgct aaaatactta tcatcaaagt aataaatata agttttataa
     3121 aatcaacttt gctaatttta aatttagatt ttattgatga tttatccttt ttaggtttgt
     3181 atttaacatc tgaggcaaat ttatatggaa ttatatgtat atctatataa tcaacttcat
     3241 caattaattt gtcaactata tcttttttaa atcttttact aaatctttta ttacttgaat
     3301 gatttctacc tattacaatt tttgttacat ttctcagttt tgcaattcta agtatttgct
     3361 ctattatatt ttcaccatgt aaaacaatta tttctccacc taatttctta gctaatttta
     3421 ggttattttg tagttgtttt tgttcatcaa atggaagtct tgctgtgttt ggagtttcaa
     3481 catataaagc tatccatttt gcaaatgtac tgtctgatat tctggatgca gttctaataa
     3541 cttttgctga agatggagat ggacttatac atgcaagaag tgtatctgat gtaggtatta
     3601 cagttacatc accctttgat agtctggtca tttgaacttc tttattaact ctctctgctg
     3661 tttttctaag tgcgatttct ctaagtgcaa ccaagttatc ttttataaaa aaattatttt
     3721 ttgctctagt agcttgttct ttcttatata tcttaccttc attgaacctt tcaagaagta
     3781 catcaggttc aacatctata agttctaact gtgtatttgt atccaaaaac ttatctggta
     3841 tagtctcttt aactgatacg tttgtaatta tctcaacaat gtcatttaga ctttctaaat
     3901 gttgtacatt tacagtagtg tagacattta tgcctgctaa cagcaattct tctatatctt
     3961 gccatctttt tgtatgcctt aacccagcaa cattagaatg ggcaaattca tctactaata
     4021 taacttctgg ttttcttgct aaagcagcat ctaaatcaaa ttcttttagt tttatagatt
     4081 tatattctat atcctttaca ggaagaattt ccaagccatc aagtaaagcc aatgtttctg
     4141 gtctagtgtg tggttcaata tatccaacta ctacatccac acccactttt tttaaatttt
     4201 gtgcagattc caacatagag taggtttttc ctactccagc tgcataacca aagaatattt
     4261 ttaattgacc tctacttagt ttttcttctt catttttaat tttttctaat agaatatcag
     4321 gattaggtct atccattaaa aaacctcctt acaccattca ttactattat aatatcaatt
     4381 ccgatataaa attgtagtta agatgtattt ttaatgatta agattatgta aagatttaaa
     4441 tatgaattta aaaaatttag ttgtaaaata aaaatgccta acaataggca tttttatctt
     4501 gtgtataatc attttagttt aagttaagta tttccataaa ctcatcacct gtaatagttt
     4561 ctttttctaa taaataatta gcaagttcat gcaatttatc aatattattt tctaagattt
     4621 caattgcatt tttgtgacaa gcctttacta ttttaaatac ctcttcatct attttagcag
     4681 cagtgtcgga agaacaagca agagaagtat ctcctcctaa atattgattg cttacagttt
     4741 caagagcgac catatcaaac tcttcactca taccaaggcg agtaaccata gctcttgcaa
     4801 ttttagtagc ttgttctata tcatttgatg ctcctgatgt acaagtgtta aagataacct
     4861 cttcagcagc acgaccacct gtaaaagtag ttattttatc aattgcttct tctttagaca
     4921 taagtacact ctcatcttca gcaacttgca tagtatatcc caatgctcct gaagttctag
     4981 gtattatagt aatcttatgg acaggtgcag aatgtttttg catagcagca acaagtgcat
     5041 gacctatttc atgatatgct ataattttct tttccttatc tgaaattaca gctcccttac
     5101 gttgataccc agcaattaca gtttcaacag attcttcaaa atcctgttgt tctactatat
     5161 ctctgccaga cttaactgct aaaagtgctg cttcgtttac aatatttgca agttctgcac
     5221 ctgatgctcc tgatgtagac cttgcaattg cattataatc tatatctgag cttacattta
     5281 cattttgagc atgtaccttt aatatagctt cacgtcctcc aaggtctgga agttcaactg
     5341 gtatacgtct atcaaatctt cctggacgaa gtaatgcttt gtcaagactg tcaggtctat
     5401 ttgtagcagc aagtattact actccaattc caccatcaaa accatccatc tcacttaaca
     5461 actgatttaa agtttgttca cgttcatcat ttccacctaa tccacttcca ttatcacgct
     5521 ttttaccaat agtatcaatc tcatctataa acactataca tggagctttt tcttttgctt
     5581 gtttaaataa atcacgaatc ctagaagctc ccattcctac aaacatctca acaaactcag
     5641 aaccagatat tgagaaaaat ggtacatttg cttcacctgc tacagctttt gcaaggagtg
     5701 tttttcctgt acctggaggt cctactaaaa gtgctccttt aggcatttgt gctcctatct
     5761 ttttatactt ttctggattg tgcaagaaat ctactatttc agttaaagct tcttttgcct
     5821 catcttgacc agctacatca ttaaatcttt ttccactttg agcagataca tacattttgg
     5881 cgttgctttt tccaaattgc atagcattac cacccattcg cttttgcata gacttcatca
     5941 ttaagttacc aaatagcata agaataccta taggtattat ccatgtaagt aaaatactta
     6001 aaagaggtga attttcctta ggaataacct tagtaaattc tacttttgca ttttttagtc
     6061 tatctaccaa ttctgggtca tccattctac cagttatgta tatattctta tccttgctac
     6121 tagatggatt tattgctatt ctattatttt ctatctcaac agatttaacc tttcctgtat
     6181 ccaacattgt gagaaacttt ccatagtcaa tctcaaccat ttttttatcc attaaacttg
     6241 gaaatattaa agcatttaaa aacattacta ctattattat aactgtataa taatagagtg
     6301 ctgacctttt ttggggttta tgttcatcca taattttatt ctcctttaaa ttatttttat
     6361 gttatttgca atcaatgtaa gttaaaagtt tatttaattt ctctaaacct tctattatat
     6421 cttccatatc ttgcaatttt tcactggtca tattttcaaa ataactatca aaaatactac
     6481 ttgaaatatg ttcttgtata tccaatgatt tatttgtaac ttttactttt acaattcgtt
     6541 ggtcttctat atctcttatg cgatttaaaa agccattttt ttcaagcctt tgacagatta
     6601 ctgatacatt actatttgtc atgtttaagt tcttccctaa atcgcttatt tttttctctc
     6661 catgttcaca taactcagat aaaatcaaag cttgcactgc tgtaatacca agtggctgat
     6721 aaaaatcact taataaagtt gtaaatttca tatttatatc tctagtcaac tctagtagct
     6781 ctttcttaaa actcatctca tccatacaaa cgctccctac gaaaatactt tatttcaaat
     6841 ttaggctgaa gtgcatactg taaataaata cttaacacta taatgtttaa tgtattaaat
     6901 attatagtat taagtattta ttgtcaatta caatattgta acttcaattg gaattttgtt
     6961 atcttattga tatttaattt tatacaggat atttttaata aaatttaata aaaaatctat
     7021 atatatattt tacactgatt tctccctttt taatctcttt taaaagttaa aatacaaatt
     7081 tattgatact ataaaggttt agtctcccat tatcgcacca tatataaaat taaagccacc
     7141 tctatctaca tacacctaaa gtgattttct taaaatataa aactactatt ataaggtcaa
     7201 atttaaaaaa aataaggtat aaagtatcat taagattaac tttataccta taaatttcta
     7261 tattttttta gttaacattt atatactctt aaaatttatt gtatcaaata gttttttatc
     7321 caatttctaa acaacaattc tgaatgtttt ttccatttta cagttggctc ttctgaaggg
     7381 tcatcattaa tatagtaatt ttgaggtata gatattttat ctcctttatt tatatccctc
     7441 ttatattctt tatcaagagt gtatttgtca tactcgctat gacccattac gaacacatct
     7501 cttgaatttg taattatata gggaccagct tctttagaat gactcacaat gctcaactct
     7561 ttattctctt tcaaagcttc aatatttaca gtagtatgtc ttgaatgtgg agcaaaaaat
     7621 tcattttcaa atccatctac tattttacta tctttatcta ccttatgctt aaatactcca
     7681 aaacattttt gacttagtgg aagttttttt acattgtagt atttgtaaag acttgcttga
     7741 gctgcccaac atatatataa tgtagattta gtttttctct tagaataatc cataatttct
     7801 tctagttcat tccaataatc aacttcttca aactccattt gttcaactgg agctcctgtt
     7861 attataaagc catcaaaata ctctcctttg gcttcatcaa atgttttata aaattcttta
     7921 acata
//
LOCUS       DAGSHT010000154         7858 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGSHT010000154 DAGSHT010000000
VERSION     DAGSHT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.159"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            24..359
                     /locus_tag="MJB72_002848"
     CDS             24..359
                     /locus_tag="MJB72_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263924.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            496..1773
                     /locus_tag="MJB72_002849"
     CDS             496..1773
                     /locus_tag="MJB72_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBY8263925.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1894..3282
                     /locus_tag="MJB72_002850"
     CDS             1894..3282
                     /locus_tag="MJB72_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY8263926.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3653..4365
                     /locus_tag="MJB72_002851"
                     /pseudo
     CDS             3653..4365
                     /locus_tag="MJB72_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
     gene            4483..5352
                     /locus_tag="MJB72_002852"
     CDS             4483..5352
                     /locus_tag="MJB72_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY8263927.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5507..6652)
                     /locus_tag="MJB72_002853"
     CDS             complement(5507..6652)
                     /locus_tag="MJB72_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY8263928.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(6986..7762)
                     /locus_tag="MJB72_002854"
     CDS             complement(6986..7762)
                     /locus_tag="MJB72_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBY8263929.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         3032 a          900 c         1286 g         2640 t
ORIGIN      
        1 aataaagaaa agaggagaat aaaatgtaca acatagatga tatgatatat aattgtccag
       61 tagaagcttt atcaaatatt ttaggaaaaa aatgggtggc tataattata tggaaaatac
      121 aagaagataa aaaacgattt ggagagctac aaagagctat accagactgt agtaagaaaa
      181 tgttagttca acaactagat tttttaattg aacaggggat tatagtcaac caaaaaaagt
      241 tggttaataa tgttgtagag tcaacatatt ttttaagtga atcaggatta cgattgcttc
      301 ctgtcatgga aaaaatgatt atgtggagca ataaaaattt aaattgtgat aaatactaaa
      361 atttactctg taagtaatga aaatttaatt aagctatgag tagaaaaatg tattaaaatt
      421 acataatttt ttagttagat tttaatttag aaatgggatt gtaatattgg aaataaaata
      481 aggaggaatt ttaatttgca aaatgtaaat ttatttttag tatttataga aggtatagta
      541 tcattctttt caccatgtat actgccaatt ttgcctattt atttgagtat attgtcaaat
      601 agtagtgtag aaaatttaaa agaaggaaag acgagtttta taggaagttc tttatttaaa
      661 aatactatat tttttgctct tggaatctca acaacattct ttatattggg ttcatcagta
      721 aaagtattga gtatgttttt taatgaaaat aaagatttaa taatgtttat tggtgggatt
      781 ataattatta tcatgggatt attttacatg ggaataatta aatcttcaat attgaataga
      841 gaaaaaagat ttaatgttaa gtttaaagaa atgaaagcaa taacagcttt tatattaggt
      901 tttacattta gttttggatg gacaccttgt attggtccaa tattggcatc tgtattagtc
      961 atggtttcaa gttcaagtaa tcatcttagt gcgaatcttt taatagcagt atatactatt
     1021 ggttttattt tgccattcat aattacagct atgttttaca gtaaactatt taaaactata
     1081 gataagataa aatctaacat ggaaattatt aaaaaaatag gtgggattat acttatagta
     1141 tcaggaatat taatgatggt aaatggattt ggaagtataa gcaaacactt taatacgtca
     1201 caaaatagta aaattgaaag caagcaagaa gaaaataagc gagaaaattc tactgataaa
     1261 gaagaaaatt ctgatggtaa tgatagtcaa aaagactcta ataatgacaa taatgataaa
     1321 gggtctaatg atgaagatag aataaagtct attgatttta ctttaacaga ccagtatggt
     1381 aaaacacata aattaagtga ttatgaaggc aaagttgtat tcttaaattt ctgggctaca
     1441 tggtgtcctc catgtaaaga agaaatgcct tatatagaac aactgtataa agactataat
     1501 aaaaataatg atgatgtagt aattttagga gtagcttcac ctaatttagg tagagaaggt
     1561 tcaagggaac atgtagttaa cttcttaaaa gaccaaggtt atacattccc tgttgtactt
     1621 gatgaagatg gagcattagc atatcaatat ggaattaatg cttttccaac tacatttata
     1681 attgacaagg aaggatatgt aactcagtat attccaggag ctatggataa ggcaactatg
     1741 gcatcattta ttgaaaatca gagaaataaa taaaagtatt taagtataag tttataaatc
     1801 aatttaaggt tacaaaaaag tgcctaattg taccacctaa ataatcttgt tatagtaaaa
     1861 gtataaacaa tacaagaaag ggttgaattt tatatgaaaa aaacatttga tgctataata
     1921 ataggatttg gaaaaggtgg aaaaacatta gcaggggatt tggcaaacag gggattaaaa
     1981 gtagctttaa tagaaaagtc aaacaagatg tatggtggaa catgtgtaaa tgtggcatgt
     2041 ataccaacaa aaagcttaga aaattcagca aacagcgtaa aaacaaagaa tataaattct
     2101 tgggatgaag tacaagctga atatgagaaa gctatagata aaaaagaaac tttaattaca
     2161 aaactcagag aagctaacta taataaatta aattcaaatg agaatgtaac tatattcaca
     2221 ggaatgggaa ctttcattga tgaaaaaaca gttcaagtca aaactgaaaa tgaaatttat
     2281 gagttagtag cagataatat atttattaat actggttcaa gaccatttat acctaatatt
     2341 aagggaattg aaaataagaa tatagtatat gatagtgagt ctcttatgaa tctaagaact
     2401 ttacctaaaa aaatgacaat tataggagct ggatttatag gtttagaatt tgctggtatt
     2461 tatagttcat ttggagcaga agttacaata ttaaactcta ataatggcat tttaccaaat
     2521 gaagatgttg aggattcaga agaaataata aaattacttg caaagagaaa tgtaaaaatt
     2581 gttaataatg caaatataaa agagattaaa gaagtttctg aattagcaat agtagaatat
     2641 gaagtagatg gtaaatctaa agaattaaca agtaatatga tacttgtagc tacaggaaga
     2701 aaagcaaata ctgaggagct agggctagaa aatgcaggta tagaattaaa tgaaagagga
     2761 tttattaaag tctcagaaac tcttaagact aataaagagc atatatgggc tataggagat
     2821 ataaatggag gtccacaatt tacttatatc tcattggatg attatcgcat agttattaat
     2881 cagttgtttg gagataagac tagaacaact aatgatagaa agaatatccc aaattctata
     2941 tttataagtc cagcattttc aagagttggt cttaatgtta aacaagctaa agaaaaggga
     3001 tatgaagtat tagtggcaaa gatgccagta gaagcaattc caagagctaa acagattggc
     3061 aaggcagatg ggtttataaa aatagtgata gataaaaaat ccaataagat acttggagct
     3121 agtatgatat gtgaaaattc aagtgagatt atccatctta ttcaattagc tgtagattta
     3181 gaagtagagt atacatattt aagagaccgt gtttatgcac atccaactat gacagaagct
     3241 cttaatgata ttctatctcc taatatgata aaagaagtat aacataagtt taatatataa
     3301 atctaaaatt atcattataa gtagatttta gaaagaggct atctcaaaaa atagagataa
     3361 gtctcttttt tactgtttaa ataataaaaa tatatttgat tttataaaag aagattatct
     3421 tatgaactaa aaagtttatt ttaaaacact acctatttat gttataaact tataaatata
     3481 atatagaatc tattattctg tattattaag tggggttaag cgttataatt aattataaat
     3541 attgaataat ctaaatggat aagataatgg tattaaaaat tatcttaaaa ggtgaaataa
     3601 tctgtattta atgtagtaca gttatatctt taaagcaagg tggtgaaatg atataaataa
     3661 gatatctaaa agagggtttg atgatagtga taaaagatgg ttttcaagta aagaattaac
     3721 aagacttata aaggtaaaga agaaatagaa tggttattaa atagggatta caaaatagac
     3781 ccagtcgtta cttttgttgg tgatagatat cagttttcta taagacaaag agatgcatta
     3841 aaaagggcag tctgtactga tgaaaaaaat attataagac aatcaaaaag gctttctcta
     3901 gataaaatca aagaagatac cgtaaatata gatggcttta atttgataat atccatagag
     3961 gtagcattat ctggtggtac tctagtaata ggaagtgatg gcaatataag agatttagca
     4021 ggtcttaggg gaacctataa gattatagat aaaacagaag aagctttgaa tctgataggt
     4081 aaatttttta ataaatacaa tgcacagaat cttaaatttt atttagatgc tccagtttct
     4141 aattcaggaa atttaaagta taggatatta gagcatgcca aaacttggcg aattgaaact
     4201 gaagtagaat tagtaaaaaa tgcagatgtt ttattggaaa aattagatag agtagttagt
     4261 agtgatgcag tcatagttga caagtgtata agttatttta atgtggctag aggtataatt
     4321 gaagagtata ttaaagaatg taatatcata aatttaaata aatagataaa tataggtgag
     4381 aatatataag tatatgtagc aatattatta gtaatacaag taaagtttga aaataggaga
     4441 acgaatacat atcgaatttc ataaaatgtt gtaaaaagac atatgagagg atggatatgt
     4501 gtgaaattaa ctaaactaaa tattaaaaaa tatagagcac ctatatataa atatgcttta
     4561 gcaaatgtaa atttgaggtc tgctaaatct actaattcaa gtatcataac agtaatacct
     4621 cagggagcta aaatggaagt attggatgaa gaagatgact ggattaaggt gatgtacaat
     4681 tctcaagaag gatatgtgta taaggatttg gtatcagtaa gcgaatatgc ttggagtaac
     4741 ttaaacttaa gagaagataa atctacaact tccaatataa ttacagtaat tcctgaaaaa
     4801 tctagggttg aagtccttca agtagatgga gattggagta aggttgtata tgatgataaa
     4861 ataggatatg tttttaatta ttttttatca attgatggga ataaaccaaa tgaattagat
     4921 tataagtatt tttatactga catgattaaa tttgttaacg aaaataacat taaaagtact
     4981 agcgattatc taattgtaac agaccttaga aataaatata cttatatttt taagaaagat
     5041 aatggaggat ggggacaact ttacaagtgg caatgtacta taggcaagcc tgagactcca
     5101 actataacag gtatatttta tataagtgga agaaaacctt cttttggaac ggatgagtat
     5161 tcagttaagt atgctactag aattaaaggt ggatattact atcattctgt actatatgat
     5221 tcaacaggaa gttatataat agatggcaga cttggtgaag ctcttagtca tggatgtata
     5281 agattaagta cggaaaatgc aaagtggata tatgataata taccagatac aacaacagtt
     5341 ataattcatt aagaaaaatt ttgagttttt tattgggata atgattctta aattaatagg
     5401 attatatatt aacttctcat aaagagtact atttaaaaag tgtaataaga gagtgctatt
     5461 aacaaaacaa agatattttg aggcactctc ttataaatat acataattaa gatattttca
     5521 taaacaattc tataatctta tctttatcaa agaaaacagg attagcacca gcacaagcat
     5581 ctttcataga gttttctgca agcaattcaa aattaggatt atcaacacct acatctttta
     5641 aagattttgg gattccaacc tcttcagaaa gagcttttat cttatctatt acaaagtcaa
     5701 cacactcttt atctgattta ttatttacat ccattccaat aacttcagct attggtcgga
     5761 atttggcagg ggctgattta gcattttcct cctcaactat tggaagtagc atggcattac
     5821 atactccatg aggtaaatca tataaacctc ctaattgatg agccatagca tgtacatttc
     5881 caagacctgc attactaaag gcaattccat ttaaaaaaca agcataacac atttgttctc
     5941 tagcttctat gtcattacca tttttaacag ctcttggaag gtatttaaat atctgcttta
     6001 tagcatataa ggcagttgaa tctgtaacat cataagcacc attagctact acagcttcta
     6061 tagcatgagt tagtgcatcc ataccagttg ctgctgttaa attagatggt ttgctaatca
     6121 ttaattctgg gtcatttact gtcattgtag ctagtgaatt ggtatcaacc attatcatct
     6181 ttacatgtct gtcttcatca gttataacat agtttattgt aacttcagca gatgtaccag
     6241 cagtagtagt tatagctatg ataggaagac attttttaga agttttgttg ataccttcat
     6301 agtctctaat atctcctcca ttagtggcta atatagagat agctttacca cagtcttgtg
     6361 gagaaccacc tcctatagtt ataacgaagt cgcaattttc tgatttcaat attttaagtc
     6421 ctttattcac attagttaca gttggatttt gtttaacttc atcataaaca acaaagtcta
     6481 catctatctg agataaaata tctgttactt tttttacagt accatttgaa actagaaatt
     6541 tatcactaac aataaatgct tttttagttc ctaatgattt aattgaatct ttagcttcat
     6601 taagacatcc tttcccaagt aaatttattg gtggtacata ataaatgctc ataactaaaa
     6661 tctcctttta ttttcattga tttttatgta aaaatatata tctaacaatt ttcactaatt
     6721 tactcataat attatcgtat cttagattga gatagggttg ctggttctta tataagaact
     6781 tgattcgtaa ttacgattat gattagaatt taacatagta tttttatcat gtcaataaaa
     6841 atataaaaaa tattataaaa aaaacaaagt tactgtagtt aaatcagcaa ctttgttttt
     6901 atagtataaa tcaaataaat acattagttt aaaacaaaat ttttaattat tatgttttga
     6961 tatcttccat atacagaata ttaatctaat ttattacaat gtcttcatta cgttcttcca
     7021 tcattgattg aatatttttc tgagcattta agagtaattt ttttatcagt tctatttttt
     7081 cgtcattagc tctaaataaa ggcagtgtta cactaacagc agctcttata catccatttt
     7141 ttcttattgg tacggcaaga cattttaggt gttccattga ttcctcacac tctgaagcta
     7201 tttgtgttct ttgaacttca agaagctggt cgtataaaac atcaaaatct gtaattgatt
     7261 tttctgtaaa accttttaga ccatctggat ataagcttat tagtttttcc ttgtcaaaat
     7321 cacataaaag agcttttcca agacctgtac aattagcagg tatttttttt ccaacataag
     7381 aaataagtct aattggttct ggagaatcta cttttccaat ataaagaact tcattattgt
     7441 caagtatacc aagctgacaa gtttcagaac aggaattaac tatattagtc atttcgtcaa
     7501 ttatatagtt aaaaatagtt cttttttcaa aaaatgaaga ccctaatgtg taagaacgta
     7561 tccctataga atatttggat gtgtttttat caatagtaat gtattttcta gcattaagtg
     7621 tatgaactat aggaaagatg ctactttttg gagcattgat aacatctgct atttctgtaa
     7681 gagtatatcc attttcggat tctgctaatg cttctaaaat atctaatatc ctagctgttg
     7741 ggcgatgatt atctgaagtc ataaatttcc ccctcttttt attaatttaa agtattagaa
     7801 tatgttgatt ttagcacact atacgatatt ttacaatgta gaaaataaat atgtaaat
//
LOCUS       DAGSHT010000155         7800 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.160, whole genome shotgun sequence.
ACCESSION   DAGSHT010000155 DAGSHT010000000
VERSION     DAGSHT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.160"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(253..396)
                     /gene="scfA"
                     /locus_tag="MJB72_002855"
     CDS             complement(253..396)
                     /gene="scfA"
                     /locus_tag="MJB72_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBY8263930.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(462..824)
                     /locus_tag="MJB72_002856"
     CDS             complement(462..824)
                     /locus_tag="MJB72_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBY8263931.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(882..1175)
                     /gene="yajC"
                     /locus_tag="MJB72_002857"
     CDS             complement(882..1175)
                     /gene="yajC"
                     /locus_tag="MJB72_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBY8263932.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1309..2430)
                     /gene="tgt"
                     /locus_tag="MJB72_002858"
     CDS             complement(1309..2430)
                     /gene="tgt"
                     /locus_tag="MJB72_002858"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBY8263933.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2509..2994)
                     /locus_tag="MJB72_002859"
     CDS             complement(2509..2994)
                     /locus_tag="MJB72_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263934.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(3006..4031)
                     /gene="queA"
                     /locus_tag="MJB72_002860"
     CDS             complement(3006..4031)
                     /gene="queA"
                     /locus_tag="MJB72_002860"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBY8263935.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEFLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4349..5368)
                     /gene="ruvB"
                     /locus_tag="MJB72_002861"
     CDS             complement(4349..5368)
                     /gene="ruvB"
                     /locus_tag="MJB72_002861"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBY8263936.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5475..6077)
                     /gene="ruvA"
                     /locus_tag="MJB72_002862"
     CDS             complement(5475..6077)
                     /gene="ruvA"
                     /locus_tag="MJB72_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBY8263937.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6070..6579)
                     /gene="ruvC"
                     /locus_tag="MJB72_002863"
     CDS             complement(6070..6579)
                     /gene="ruvC"
                     /locus_tag="MJB72_002863"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBY8263938.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6907..7536)
                     /locus_tag="MJB72_002864"
     CDS             complement(6907..7536)
                     /locus_tag="MJB72_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263939.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
BASE COUNT         2427 a         1449 c          854 g         3070 t
ORIGIN      
        1 agagtttaaa attaaaaatt attctaatac atgtaattga taaaaaataa aatataagag
       61 tagctacttt aaaataaatg ctcttatatg attagatatt tttgcataat taaacgataa
      121 gaaatcaaat ttaattttac ataaatagaa tatatatttt tatataataa ttgaaaataa
      181 aagaggatac ctcataatga taaaagaatc actttgagat accctcttac aaacaactaa
      241 atcaataatt ttctatcttt cacactcttg atttgcaact gtacaagaag ttttacaagc
      301 tgattgacaa gaagtttgac actctccaca tccacctttt gcagcacttt gctttaaagt
      361 agcttgagat aaagttttta catgtttatt ttccattttc aacgcctcca tttctaattg
      421 tttcctattg attatatcat aaaactactt taatataaac tctatttaaa gttgacaccc
      481 aatacgcctc caatgcctcc tgctgctgat attaacaata acttataaat catccctact
      541 tcaaatacaa acttttcttg tgctaaaaac acagtcaaaa aaatgcatac tatgtacaaa
      601 agtcctacta aaagcccata cataagtcct ttttctttca tgtgttttga agtgtaaaac
      661 cctcctatag ctgcagaaat agtagttata aatgatgaga ccatagttat attatctcca
      721 ccaatatctg taaatgtcaa aaataagtta tacactaata aaacagccaa agttattata
      781 tatgcatatc ctagaccttt aaaaatataa ttagattttc ccataaaaaa acctcctctc
      841 aaattaatat atattcgaga aaaggctttt aaattactaa tttagtcttc ttctatttct
      901 tcttttttag atttaacagt tcctatagcc catttttcaa atggaacttt agttctattt
      961 ggtcctaatt ctaatattac aacatcatct tcaactttag ctacattagc tactattcca
     1021 cctattgtaa tgaccttgtc tcctacactt aggctacttc tcatttcttt taattgtttg
     1081 tcttttttct tttgaggtct aatcattagg aaataaaaga ttgcgattac tactacccat
     1141 agacctatac tcataattat ttgttgctgt ggcattaatt tttcactcct ttgtaaatta
     1201 tactcttttg taaatcataa taaatgttta tataacaatt tactttctct atctttgagt
     1261 actattgaat attatatatt aaaatgatta taattaatag tgcaaaacct atatttcata
     1321 tccatatttt gcaaaaaatt catttctaaa atcaagtaat ctatcttcca ttattgcttg
     1381 tcttatttgt ttcattaaat ttaataaaaa gtgaagatta tgagtagtta ttaatcttgc
     1441 acccagtatt tcatttgctt ttataaggtg tcttatataa gctcttgaat agtttttaca
     1501 agcataacaa tcacattcag ggtcaagtgg agtaaagtct tcagcataag ttgcatttct
     1561 aactactact ttaccttggc tagtcattgc agttccattt ctagcgatac gagtaggtag
     1621 gacacaatca aacatgtcta ctcctcttat aacaccttct actaagtcat ctggactacc
     1681 aactcccatt aggtatcttg gtttatcctt tggcataagt ggagttgtat gctctaacac
     1741 gtcatacata agtggttttg gttctccaac actaagtcca ccaatagcat atcctggtaa
     1801 atcaatatta gttatttctt tcgctgattg ttcccttaag tctttataca tcccaccttg
     1861 tattatacca aataaagctt gcttgtcggt attattatgg gcatctttac atctttttaa
     1921 ccatcttgtt gttctttcta aagaattttt tacatattct ctatctgatg ggtatggtgc
     1981 acactcatca aatgccatca ttatgtctga acccaatgca ttttgtattt ccatagcttt
     2041 ttctggagtt agaaaatgtt ttgaaccatc taagtgtgac ctaaattcta caccttcttc
     2101 ttttatcttt cttaaaggcc ctagactaaa tacctggaac cctccactat ctgtaagtat
     2161 tggtttatcc caattcataa acttatgaag accaccagct ctttttataa gttcatgacc
     2221 tggtctcata tataaatggt atgtgttact aagtattatt tgtgaaccaa tctcttttaa
     2281 ttcttctggt gtcatagatt ttacagttgc ttgagttcca actggcatga atattggtgt
     2341 ttcaataatc ccatgtggag tgtgtaatct tcctaacctt gcaccactct gcttacaagt
     2401 ctttatcaat tcgtatctta ctgcgtacat aaagttctcc ttaatcatat ttatttctca
     2461 ttatttctta agatatattt gtaataagct attcatataa tttattgtct aaatagattt
     2521 atcttcgtct tctttgttct gttgttcctt aattgcttct tcatcatgaa tactcattat
     2581 attaagagca ctcttgtctt ttgttactaa aaactttttt atagcaaata gtcctgctat
     2641 agctataact cctattaaaa catctatcat accttcattt ttaggcagta gcaacatttt
     2701 tctagctatt gcgtaaagta atgcctcaat aaatgctcct ggtatatgaa gagatagcat
     2761 tactacaagt tcaacaccta ttactaaaag taatatttgt gctaaaaaat cattaagctg
     2821 actgtattgt actggatttt gaaaatcaac tatatatgct ccccacataa gtcttagaac
     2881 atctatagtt cctaaaaata ctgctattag aactactact gctaaaacac gttcaaatat
     2941 aaaagcaatc tttaatatag ggttattctt tttttgtttt tgtaaactat gcatccatta
     3001 cctccctact ttataatcat agcatctcca aaactgaaga atctatatct ttcttttact
     3061 gcctcattat atgcatttaa aacattatcc tttccacaaa tagcacttac taacattatt
     3121 agtgtagact ctggtaagtg aaaattagta ataagtttat ctaaaacttt aaatttataa
     3181 cctggatata taaatatttc tgtccatcca ctagtttctt tcatctttcc atcctcatta
     3241 caagctgatt ctatagttct acaactagta gtaccaacac atattacatt ttttccattt
     3301 tcctttgctc tatttatttt gtcagctgct tcttggctaa ccatataata ttctgaatgc
     3361 atcttgtgat ttaaaacatc ctctactttt actggtctaa aagttcctaa gccaacatgt
     3421 agagttacaa aggctatatc tacacctttt tcttttattt tatttaacaa ttcttctgta
     3481 aaatgtaatc ctgctgtagg tgctgctgct gaaccattat gctttgaata aacagtctgg
     3541 tatctttctt tttcatctag tctagcagtg atatatggag gtagtggcat atttccaagt
     3601 tcatccaata tttcttcaaa aattccatca taatggaatt ttataattct tgaaccttca
     3661 tctgataaat ctacaacttc tccaattagc tttccatttc caaaagaaaa tttagtccct
     3721 acttttgctc tttttcctgg ttttactaaa gcctgccaag tatcttcctc tgttcttttt
     3781 aataataaaa actctatttt acctccagtt tcaagcttct ctcctattaa tctagctggt
     3841 ataactctag tattatttaa tactaagcaa tctcctggat ttaaaaattc tattatatct
     3901 ctaaatactt tatgttcaat attacctgtc tctttatcta ataccatcaa tcttgagcta
     3961 gctctatctt ctataggaac ttgagctata agttcctgtg gtaagtcaaa tttaaaatca
     4021 cttgttttca atgttaaact tcctctctta aaatttctcc agctacaata atatcaaaaa
     4081 aacaacttat tttccacaat attaccataa tctatcaaga aattgctatt tttaacagac
     4141 accatatcca caatatatag atatttttcc acgatataca tgtcttatcc acaatatata
     4201 gatacaatac acacaatttt tatcgtgttt atccacagta tctacagata taattaccat
     4261 tttgtggata tgtttgaatt tttagccatt ttagaaatat tttaatttaa tgactctttt
     4321 attttataaa tactcgtata atctattttt atttttcatt aggatatgga atttttaagt
     4381 gttcatatgc taatggcatg gctactcttc ctctaggacc tctatttata aatcctaatt
     4441 gaagcaaata tggctcatat acatcttcta ttgtatttct atcttctcca atagaagctg
     4501 ccaatgtatc taaacccact ggtcctcctc taaatttttc tattatagtc ataagaagct
     4561 tctcatctac aaaatcgaga cccaaactat cgactcctaa aagttccaga gcatcttttg
     4621 ccaccttatc agttattttt ccatcagctc taacttgtgc aaaatctcta actcttttta
     4681 gtaatctatt tgctatcctt ggagtccctc ttgaacgttt tgcaagttct aatgctccat
     4741 tagactgtat ctctgcatct aagatgcttg aagacctaag tactattttt gaaagttcat
     4801 caactgtata atagtccaat ttacaaataa caccaaatct atctctaagt ggatttgtaa
     4861 gcataccagc tcttgtagta gctccaataa gtgtaaattt aggaaggtca agtcttatgc
     4921 ttctagcaga tggccctttc cctattatta tatctaggca aaaatcctcc attgctggat
     4981 ataaaacttc ttcaacactt ctattgattc tatgaatctc atctataaat aaaacatcat
     5041 tttcatttaa attcgtaagt atggctgcca aatctccagc tctttctatt gcgggtcctg
     5101 acgtaattct taaatttacc cccatctcat ttgctattat acttgctagt gtagtttttc
     5161 caagacctgg tggtccatat aaaagcacat ggtctaactg ctcatttctc gatttagctg
     5221 cttctataaa tatactaagt tgttctttgg atttttcttg acccaaataa tcttctaaag
     5281 tttttggtct taaactattt tctatatcta tatcttccat cttcattgta gaagttatta
     5341 tcctattttc atcttcaaaa ccttgcattt atacccctcc tctcatactt ttgaaaatct
     5401 actttatata ttttcaaatt tacataaact ccttaaatat tgttttaaaa tccaaatatt
     5461 ataaaatcca aatactattt caaggattta ctcattatat atgtaagtgc tttctttata
     5521 atagcttctg tattcatacc atctttttta catttttgaa ctgcctcttt tgattccgac
     5581 aaagtatagc ctaaagctac aagtgcatct acagactcat cttgtgatat caaggtaggt
     5641 ttttgtgata ataacgttgg ttcaaactct atgttatttt tgtctacctt atccttaagc
     5701 tctaagacga ttctttctgc tgtcttttta ccaacaccag gagctttgga taattttcca
     5761 atgtcttcac ttaaaatata tgctcctaac tgtgctggtg atgcaaatga tagtattgaa
     5821 agcgccactt taggtcctat tttagataca gatgttagta attcaaacat cttaagttct
     5881 tccctactta caaaaccaca aagactcata tcatcttctc ttacaattaa tttagtatat
     5941 atttttgtgg cttctcccac ttgtaaattc attattgtat tggatgatac atttatttta
     6001 tatcctatcc cattattttc aacaactata ctgtctatat aaatttcttc tactgtacct
     6061 tttatataac tatacatact taccacctat attctttaga gttttttcaa gtttgtttga
     6121 atgagcatgg caaatagcca cagctaaagc atctgcaaca tcatctggtt ttggaacttt
     6181 ttttagactt aaaaatgaag ttaccatctg ttgaacttgt gccttatctg ctctcccata
     6241 tccaactaca ccttgtttta cttgaagtgg agtgtattca tatacaggct tcccattatg
     6301 agcacatgca agcatagtaa cacctctagc ttgtgcaact gttatagctg tttttacatt
     6361 cttgttaaag aataattctt ccattccaac ttcatctata ttgtaattct ttactattgt
     6421 atcaattcct ttatacacaa gttccaatct tctcgatata ttcatatggg caggtgttgt
     6481 aactgctcca taatcgatta ctttaaactt gctatttttg tattcaatta taccatatcc
     6541 aactatggct atacctgggt caatccccaa tattatcatt ttttaaaatt ccccctaaaa
     6601 ataaaagaaa taccaaaatg tatttctcat aaaaacttat gttctaaatc attatatcat
     6661 aaataatatt aacaaatcat aaaatgctct atgtaaaata taaaatcaaa taaaaaagtt
     6721 atatcaaagc aagattttac ctcaatataa cttttatatt tattacaaat aaattattgt
     6781 atttttatag gaattaaatg tgactacaat tttaatacta atctatttaa tattaaatta
     6841 tttattatta atttacttag tatttaactt acttagtatt taacttaaaa taatattaat
     6901 ttactattaa cttgcaaagt cttctaatct tgaataggcc tcttcttcag tatccataac
     6961 tacacctttt ttgatttcat cttgaactgt tttaggaagt gaatctactc ttatttttgt
     7021 tttttctact aatgttttac ttccgctatc atcatattta tatagtgcta tatttccatt
     7081 atcattttca atagtatatt taccttttat agtaacatcc attgcttgta catctttatt
     7141 atcactatat ttagattcta ccaatatgat ttcattttta tccatactct ttattttttt
     7201 agttggatat tcttttttaa aatatgatac tatttctgac ttcgtcttat ctaataatga
     7261 ctctggaact attcctgcca ttgtaagtgt tctcttatag ttagtttcat tttcgccttc
     7321 actttgaagc caaacttcac aatttttatt taatccaaaa gcttcttttg acttttcatt
     7381 catattcgca ctagtactta caacttcacc agtttgaact ccattgtgtt ttaatcttgt
     7441 ccctatataa actccgctac ttaatacaaa tactccaacc agtattaagg cagatatttt
     7501 ccaaggaaat aattcttttt tcttcttttc aaacattgat tacgcctcct ttttctatta
     7561 ttttctccaa gctgtaagtt tttatacaat ttaggtaaat tttagaagaa aatatattta
     7621 tatttcaaat tactctaaga atagatattt tctaaaaggt agttttctct taatggttat
     7681 attcttgtaa ttatatggac ataatttata ataaataatc gtttaggagg taataacttt
     7741 ggaaagaata aactgtaatg taattaactg ttcacataac aacagccata tatgttatgc
//
LOCUS       DAGSHT010000156         7800 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGSHT010000156 DAGSHT010000000
VERSION     DAGSHT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.161"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            950..2308
                     /locus_tag="MJB72_002865"
     CDS             950..2308
                     /locus_tag="MJB72_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBY8263940.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2415..3209
                     /locus_tag="MJB72_002866"
     CDS             2415..3209
                     /locus_tag="MJB72_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY8263941.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3202..5508
                     /locus_tag="MJB72_002867"
     CDS             3202..5508
                     /locus_tag="MJB72_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY8263942.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5647..6924
                     /locus_tag="MJB72_002868"
     CDS             5647..6924
                     /locus_tag="MJB72_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8263943.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7107..>7800
                     /gene="yqeC"
                     /locus_tag="MJB72_002869"
     CDS             7107..>7800
                     /gene="yqeC"
                     /locus_tag="MJB72_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBY8263944.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGS"
BASE COUNT         3069 a          842 c         1456 g         2433 t
ORIGIN      
        1 gtatatttta tgttgcagat acaaatatag taaaggagag tacattggta caactgagat
       61 acatagctta ttaagttttt ctgtcttatg agaattatat tataaaatat tcataaatat
      121 tttgagttta aattaaataa ttattttatg cttagattaa ttgatattta attaatttaa
      181 taacaaaaac agtgaaagag taaataatat tatttacagg gggtaaagca aagtatgtta
      241 aataaattgt acttttggcc ccgtacatag agaaatctat gtaggacaca actttaaaac
      301 cagtaaatcc taaagccttg caactacaat aaaagataga aagaaacttt tatgaatgtg
      361 gtgaaaacca gaaacaatag tgaggatggt atatggtgag agcctaagta cttaaaaatg
      421 gacatttggt cgccaagcct agaaatagga aggtcaaacg actatctcga gagagagtac
      481 actataagcg attgatagtg gaaacgggtt gctcttagca ggtaatgctg aagatgaaga
      541 aatagtctac gctcatatga aagtatgagg tcactggatt tgaagtgaca tctataaagt
      601 agcgtttata ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa aacttataac
      661 ttataatgat taagattgaa agatttaaat tgaattaaat ttactataaa aaattaaaaa
      721 ttcatagcta taatactctt atatttataa agcatggtat ataatgtata caaaattaaa
      781 gaatacgtgt atttgttaat aattaaattt attgcatctg ttttttaaga ctaaaaactt
      841 aaatatatgt tggagtatag ttgatattta tgaacatcat taatttaata tattagattt
      901 ttaagtattt atataattca ataagtttta tgtaacggga gtttttatta tgaaagcaaa
      961 aagtacatca aaatttgaat tagacggaat accaccacta aaggaagcaa tacctttagg
     1021 gttacaacac ttaatggcaa tggtaatagg tagttgttta ccagcgatat tgattgcaaa
     1081 tgcagctgga cttaatcata agatgtcaac attattgatt caagccgcat tagtttttgc
     1141 aggattatca actttattac aattatatcc aatacctttg tttaaaggtt ttaaattagg
     1201 aggaggaata ccagttataa tgggagctac agctatgttt ataggagctg gggtatctgt
     1261 tgcaagtaag tatggactac cagtattatt tggctctcaa ataatagctg gtatagtaat
     1321 aattataatt ggttttggtc ttaaaaaaat acgatttata tttactccag ctgtatcagg
     1381 aacaatagtt gcatgtatgg gtcttggact ttttccaata gctataaaaa acttagctgg
     1441 gggtatgggc aatgagactt ttgggaatcc aattaatttt atagttggaa tatctgtatc
     1501 tcttatgata ttaggattaa ataagtatgg aaaaggatta tttaaagatg catcaatatt
     1561 agttagcata atttttggat acattttatc attaattttg ggtatagtaa atttttcttc
     1621 aattcaggag tttactttgg ttgcacttcc aaaaccactt gcttttgggc tagatataag
     1681 acttgaagtt gttgtaatgt tttcaataat atatttagtt gagatagctg atataatggg
     1741 tgcttgtaca ctttctgcgg taggagggct taatagacaa gtaacagatg aagaactttc
     1801 ttctgctgtt ttaggaaatg gagtactctc tacaataggt gcattatttt ctgcaactcc
     1861 aatggggatg tttggacaaa attctgcaat cgtttccaat acaaaggttg taagtaaatt
     1921 tgtactagct attggaggaa ttggattatt tttggcagga atatcgcctt tattagctaa
     1981 tttaataaga acaattcctc cctgtgtggt tggaggagca acactcgtta tattttcaac
     2041 tcttacaaca tctggtttaa gacttgttag tatggatgga tttaatcaag aaaatagcat
     2101 gatattaggt ctttctatgg cttctggtat tggattcatg gtagctcctc aagttcttga
     2161 aaaatttcca aaatttatag agacattact tgcagattca agtgtagttt caggagcaat
     2221 ggtagccata ataattcaag ctttatatat ggtaaaattt cctggaaata aaagtgataa
     2281 ggttgaggat aaaaataaaa gtttataatg taaaggttta atatatgtga attaaatata
     2341 ctacaattat aaatataaat aaaagattta ttaaaaactt ttgtgtttta ataagttaat
     2401 gatgggggga taagatggtt actataaagg aatattcagt tccacagagt ttagaagaag
     2461 catataaaat tttaatttct aaaaaaaata atgttatttt aggtggatgt ggatttataa
     2521 aattaagtaa taaaaatata ggaacagcga tagatttaaa agatataaat ctaaattata
     2581 taaaagagga taaaaaaaat attttgatag gtgcagatac ttcacttaga agtttagaaa
     2641 ttgataaaac tataaaaaat tactgtagtg gaatactttc aagcgctgtt tctaatatag
     2701 ttggtgttca atttagacaa ggggctagaa ttggtgcaag tgtattttca aagtatggct
     2761 tttctgactt aataccagcg cttttggttg ttggagcgaa ggtgagatta tacaataaag
     2821 gaatactaga gttaagtgag tttttggata gtgaattaag tagggatata ttggtcgaag
     2881 taatactacc aaaaaaagat gcaatagctg tttttgactc aataagaaaa tgcacaggag
     2941 atttttctgt attaaattca gcaatgctaa aagaaaatga tacttacaaa atagcaattg
     3001 gggcaagacc tcaaaaagct agattagcac ttgaagcgag taatatatta aataaagaaa
     3061 aagatataga taaggcatct attgttgcta gtaaggaact tacttatgga agtaatatga
     3121 gagctagtaa agagtacaga aaggatatgg caagtgcatt agttaaaagg atgtataatg
     3181 ctattgagga gggaatgtat aatgattaat aaggggataa gaaagataga tagtacagca
     3241 attgtcacag gaaaacctct atatactgaa gacctcatta tgcataaaga tgttttgaca
     3301 ttaaaactac ttagaagtcc acatgctcat gctaagataa agaatataga tacatcaaaa
     3361 gccgaaaaaa ttccaggggt tgtgggtatt tatacgtata aagatgtacc acaggtcaga
     3421 tattgtgtgc caggtcaaac agaaccagaa gcatcacctt atgatagagt tttacttgaa
     3481 gatacagtta gatttattgg agatgaagta gctttagtgg ctgctgaaga tgagaaaacg
     3541 gcacttaaag ccttaaaaat gataaaggta gattatgagg ttttaaagcc tgtgtttgat
     3601 gcaagagaag cagaaggaaa tgaagttgtt atacatccag aaaaagacat agtagagcca
     3661 tatccttttg gattggatgc aaaaaataat atagtatcaa aagaattttt tgaatttgga
     3721 gatgttgaag ctaagcttaa agaaagtgat tttgtggttg aatcttcata caaaacccaa
     3781 agtcaatcgc attgcatgat ggaaactcat aggtcatatt catataaaga tataaatgat
     3841 agaattgttg tagtttcatc attacaatca ccacacaatg taaagagaat acttagaaaa
     3901 gtattgggta taccatcttc aaaaataaga gtaattaaac ctagaatagg tggtggattt
     3961 ggaggaaaga atatagcagc aacagatata tttactgcat ttgttactat gaaaactggt
     4021 agaccagcaa aatttgtatt tacaagaagt gaaacttttg taggttctaa tagtagacat
     4081 gaaatgtttt ttgatgttaa agttggtgca gataaagaag gaaatttaaa agcaatagag
     4141 ctaaagtcat taaacaatac aggtgcttat ggtgggaatg gtgtctctgt ttcttccgaa
     4201 tctggacaca ataccttacc aatatataat gatattgatg ctgttagatt taatgctaga
     4261 acagtatata ctaataggtt accagcagga gcacttagag ggtatggtgc tacacaggga
     4321 atatttgcaa tagatagtgc tattgatgaa ttggcacata agattggaat ggatccactt
     4381 gaatttagaa ttaaaaatat aatagatagg tataccaatg gaaaagcagc tgaagacgaa
     4441 atattaagtt gtgagctgag acaatgtata gaaaagggca agaaattaat taattgggat
     4501 gataaatatc catgtaaaga tataggaaac aacaaagtaa gagcagtagg tatggcagta
     4561 gcaaatcatg gttctggtat accgcgtgta gatatggcaa cagtcactat aagaatggat
     4621 gaagatggca catataaact attatctggt cttgctgact taggtcaagg ggctgataca
     4681 attcaagctc aaattgctgc ggaagcttta aatactactg tagatagaat ttctatttat
     4741 acaggtgatt cagacttatg tccctatgat acaggagcat ttgcttcttg tacgacaacg
     4801 gttacaggaa atgcaacaat aaagacagca aataaattaa aagatatatt actttcagtt
     4861 gcagagtcaa agctaggatt taaaaaagaa gatttagagt tgggaaaaga cagggttttt
     4921 gttaaaaacg ataatagcaa gtttgttcta ttagaggaat tgggtagaga atctgttgga
     4981 ggaataggat ttgctagtga tgatgtacca gtagttttgg aagcaacaga atcttttggc
     5041 atgttgaaaa aaataaaacc atttatagca ggatttgcag agatagaatt agataaaaat
     5101 acaggcgaat ataaagtcat aaattttgca tgtgtacctg attgtggaac agttataaat
     5161 ccaactttag ctagaataca agtagaaggt ggtgtgatga tgggtatagg gtttgcaatg
     5221 tatgaagacc ctatatttga taaagatggt agattattaa cagatagttt cctacagtat
     5281 aaagttccta caaaaaaaga agttggaaat atattggtag attttgctga gaatttcgac
     5341 ccaggaggtc cttatggtgc taaatcatta ggagaaatag ttatacatac accagcacca
     5401 gctatagcaa atgcaatatt taatgctaca ggagttcgtt taagagagct tcctatgact
     5461 tttgaaaaag tttatatggg tatgaaaaat aataataaac aattataata aataataaga
     5521 tgtataaaag caagtaaatt taaattattt tgtaaaataa agtaattaaa tggacattat
     5581 atatggtgtt ttgtaaaaat accgtatata atgtctagta gagtatattc taaaggaggt
     5641 gagttcatgt caaacagaat aaaaaatata cactatggaa ctcagagaca acaattaaaa
     5701 aacttaacta aaaaagaata tcaagctcta cgagaaatgt gcttcttagc taagaactta
     5761 tataatgttg gtacatataa tgtaagacag cattatttca atcaaggaga acatcttaac
     5821 tatgaagcta attatcatgt atgcaaagaa aatgagaact ataagcttct caatagtaat
     5881 atagcgcaac aaatattaaa agaagtagac agttcgttta ggtcattttt tggattaatt
     5941 aaattagcca aagaaggaaa atatgacttt agagggataa aatcacctaa atatttagat
     6001 aaagaaggat tttttagcat aataataggt caaataagaa tcaaagaaga tgggatttta
     6061 aatgtaccta tgtctccagc atttaaaaaa ctatatggga aagtaagtat aaaagttcca
     6121 caaaatatac tagagaaaaa aattaaagaa ataagaataa tacctaaaca taaagctagg
     6181 ttctttgaaa ttcagtattg ttatgaaata ccaaaatcag aggaagtttc aaatcaaaaa
     6241 aatgcactag ctatagactt gggtttagaa aatttatgta catgtgttac caatttaggt
     6301 gatagtttta tcatagatgg aaaaaggcta aaatctgtga atcagtgggc aaacaaacag
     6361 aatgataaaa tagaagcctt aaaattaaaa aagaatataa ctgcaataac aagaaaacaa
     6421 tttaaagttt ggaataagag aaataatcaa gttaaagact atgtaaataa aacctgtttt
     6481 tatataattg attattgtgt aaaaaataat attggaaatt taattgtagg atataatgct
     6541 aagttagaaa aaagtgataa tatggagaga aaaatatata gaaattttat aagaatccca
     6601 tttggagaga taaaaggaaa attagaatat ctgtgtaaaa taaaaaatat caattttatt
     6661 aggcaagaag aaagctatac tagtaaatca gatttttttg ctgatgattt tctacctgat
     6721 atcaatgtgg acaatgttaa aaaatatgac tttaaagggg aaagaataac gcgtggacag
     6781 tacaaatcca gtgttggaat cattataaat gcagatgtaa atgcgagtct taatatacta
     6841 aaaaaatcac aagtagttaa tttatctaat ttacagaata atcctcaaat attaaagcaa
     6901 ccacagagag taagaatatc ttaattatat ttaaaagata aactgatagc gtaagcttac
     6961 agcttagcgg cgaattggat ccgccttgtt gtttacaaca gaatctattt actttcgagt
     7021 ttatggaatt tcaaaaatca cttttactaa aaaggtctaa ctataataat taagagatta
     7081 gaaaactggt tataagaggt gaaagtgtgg aactatcaaa tataatagaa aagaatgaaa
     7141 taataactgt agttggagca ggaggaaaaa cttcgcttat aaactatttt gcaaattttt
     7201 atagagataa attgaaagtt ctccttacaa caacaactaa aatatatgta ccaaacgatt
     7261 atgacaatat aattattaca atagatggaa ctgttattcc atctatttgt catggaataa
     7321 cagtctgtgg cagttatata aataatgaaa ataaattagt aagtattgat tcctctattt
     7381 tggatgaaat tgtagaccaa tttgatttag ttttaataga gggagatggc tcaaagagaa
     7441 aaaaacttaa aggttggaat gctaaagaac cagtagtata tcataaaact acaaaaacca
     7501 tagggatatt agatataaca tcatttggaa tgaatattaa tgaagagaat atacatagag
     7561 tagaaatatt taaaaaaata gcaaatttag atacaagtag tattaatagt agttctacag
     7621 taagtattga aaacttaaaa aatatagtat taaatccaaa tggattattt aaaaattatt
     7681 ctggaaaaag agtattattt attaataaag ttgaaaatga aaaatataaa aatttagcta
     7741 taaaattaat agaaaatatt aaagaatatg aaagtgaaat agagatattt tatggaagtg
//
LOCUS       DAGSHT010000157         7668 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.163, whole genome shotgun sequence.
ACCESSION   DAGSHT010000157 DAGSHT010000000
VERSION     DAGSHT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.163"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(259..1413)
                     /locus_tag="MJB72_002870"
     CDS             complement(259..1413)
                     /locus_tag="MJB72_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8263945.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSFLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(1416..1883)
                     /locus_tag="MJB72_002871"
     CDS             complement(1416..1883)
                     /locus_tag="MJB72_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8263946.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(1897..2385)
                     /locus_tag="MJB72_002872"
     CDS             complement(1897..2385)
                     /locus_tag="MJB72_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263947.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(2399..3730)
                     /locus_tag="MJB72_002873"
     CDS             complement(2399..3730)
                     /locus_tag="MJB72_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY8263948.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(3744..5309)
                     /locus_tag="MJB72_002874"
     CDS             complement(3744..5309)
                     /locus_tag="MJB72_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY8263949.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQAYE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(5479..6264)
                     /locus_tag="MJB72_002875"
     CDS             complement(5479..6264)
                     /locus_tag="MJB72_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8263950.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(6431..7630)
                     /locus_tag="MJB72_002876"
     CDS             complement(6431..7630)
                     /locus_tag="MJB72_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBY8263951.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         2716 a         1322 c          781 g         2849 t
ORIGIN      
        1 aaataaatcc ttaagtaaat attacatttt aactgttagc attacacttt tgtaacatat
       61 ggaagactat tataattata gtagaaatat cttaaaataa aaacttgctt cacttttttg
      121 tataaacaaa gaaaagaagc tcctaagaag cctctaatct atacaataat tcttctctat
      181 aaaaaaccaa aatacacatt gattgtggca aatacagata ctataaacaa gtgatattac
      241 tactatatat taaaacaatt attcctgaag ataattcaat aagactatca agcaataagc
      301 taagcatcca aaaagactta ataccattaa tgtataatta tacccaatat taatgctcaa
      361 aaaaccagca acagttggaa taatcagaaa tgaacctccc ataaatatac ttaaagctat
      421 taattggcta ctacttctaa gttttttagg agaaacttca aatattaatc tttttgaagt
      481 aattaaaatt aaaggagtag aaaacacttg aaaaacacac agtataataa tgatatttga
      541 atcatttgtt aaagcaaata atagaaactg aactgttatc ataatagcac ttaattttaa
      601 taagtttaaa ctgctatatt tttttaataa ataagaacca gccagatatg ttggtatctc
      661 taacaatgac tgtaaagacc actttaaaga tatctgagaa tttgtagcat ttaaagctat
      721 cattttatct attacagtac agttatttgc taccaccatt gagtataata agaataaaat
      781 acatactagt aagatatatt ttttatcttt taataattca ataatatctt tattagttac
      841 atttattttc ttattaatct tttcaatatc aggtaataat agtatattta taataagtaa
      901 tactacaatt aaaccaatcg ctacagataa tccaaaatat gagaatctaa gaatcaagta
      961 agatgctacc atacttccaa tagcccatcc aatcgaccca aaagtcttaa taaatgacaa
     1021 ataattatta atattatctc cacaacctat tatccatgta tcatataaac tacagcaagt
     1081 atttaataat cctcctgata aagcaacaag taataagtat attataaaag ttgcagattc
     1141 agtagaaaac ataataactg atgcaattcc ataaactgat aaactaatta aaacaatttt
     1201 tttcattgtt tgatacttat cagctaatac tccaaataat aattgaaaca atatattggt
     1261 tattgcatag ctcgataata taattcctct ttcaaacgct gagtaaccta attttgataa
     1321 gaaaggtgtg aattttgttg aagcaaaggc aaaaataata tatgagaaaa aatagaatat
     1381 gataaattta aatttaatcg ctttattaat cataatcaat caaaattgat aactttaata
     1441 ccactatttc cttcctgatt taagtttgaa agatgatatt ttaccgtatt aggattctct
     1501 acaataacta aaggaatata taaagtattt tcaagtaact tattatcaat ttctactaac
     1561 ttttccccca tattaacttc ttgaccttgt ttaacttcta atttaaagtt ttcttttttt
     1621 gttgttgcac tatccaatcc aatatgaata agaatttgaa catcattttg caatttaatt
     1681 cctatagcat ggttagtttg agcaataaaa cttattactc ctttagtcgg tgatttaaga
     1741 atattgtcct ttgctatcat agcaaatcca tctcccatta ccttagttga aaatacttta
     1801 tcaggaattt ctccaagatt aattattttt ccatcaatgt atgaattaat tgtaattgct
     1861 tctttttttc ttttaaagaa cattatagac actcctctac ataattaact ttattctaat
     1921 tatcattaca aataacgata ttaagcatac tgcaattgag gaaaaaagag ccttatagct
     1981 ctttccccat tgtagttctt taatactgct tatgatttgt ttaattccaa caaatttata
     2041 aatataggta aacatgataa caatagttat tgtgtaaatt tggaatatct gccctaataa
     2101 gactaaatag aaagcaatca tgttttcttt tatctcttta ttctttttta tattgtaaat
     2161 aacatatgca ctgtatactg cactaaagtt agagattaaa tttaaacttg cattaacatc
     2221 attgtttaaa agttgtgaaa atgaaatatt tgtaaataat gatgtcaatt ctaaatacac
     2281 tatatataaa acaggtaata ttagaaaaca aaccataacc actgaaagaa atatattttc
     2341 atcttttaat actgctgtga tattttttat tttatttgtt atcatgtttt tgttctcctt
     2401 atttaaaagt tggccagaaa tccttattag ccacaatcat ttcatctaag atttcttttg
     2461 ctacttttgc acttggaaca gttttactca tagtaaacga ctgccataat ttttgataac
     2521 ttttttctat ccatgcttct acagctaatt tttcagaagc aacttgttgt tccattaaac
     2581 ctttttggaa agttggaatt ttacctatag acataggttc aaccatatct tttccaacaa
     2641 ggcaaggaat ttcaaccatt gcatcatctg caaaattttc aatagctcca ttattaggaa
     2701 caatcagtaa aaatctttcc tgagtattaa atgctaatgc acaagctaat tcaacaataa
     2761 attgagaatg ctcacttgca tcaaaccatg tatcccttgc tgtgccattt tttataattc
     2821 tttcacattc ttcgaatgta tccttttctc ttctatccat aatttcattt gctcttgtat
     2881 aatttggatt agcatgttca acagtttcat cagccattaa ataatatttt aaataagtat
     2941 taggtaatgt agtcggttct atttccaata aatcctttgc tgctctcata gtttctaacc
     3001 aactagattc tttgtgttga ccttgaggtg taaattcctc atatcctttt ttagcacaat
     3061 attcttgtaa tttaggcatt aaatcatttc cttctttgtc cttaatactt gtccaccacc
     3121 cgaaatgatt caatccataa tatctaacat caaagtcttt acgagaattt aaacctaaaa
     3181 ctctagcaaa tatttcctct aaacatactg gcatatcaca aatattaata attcttgaat
     3241 ttggtcttaa tacacgacat gcttctgcaa ctatagctgc tggatttgaa taattaagca
     3301 tccaagcatt tggagaatat ttttccatat aatcaatcat ttcgattatt ggacctattg
     3361 ttcttaatcc ataggcaatt ccacctgccc cacaagtttc ttgaccaaca accccatatt
     3421 ttagtggaat tttttcatct tgttcacgta aagcatataa accaacacga atttgtgcca
     3481 ttacaaaatc aatatcagta aatgcttctt ctggatttgt tgtataacta aatttaattt
     3541 ctggattttt ctctctaaca attattgcac atgcttcagc aacttttctt tgtctctcat
     3601 cattattatc atataattta attgattcaa taggaaattt agcaacactt tcaattaaca
     3661 ttgttacaat tccagcagta taagtactac ccccaccagc aattagtatc ctctgtcttt
     3721 ttctttccat ctttccttct cctttataat aaattttcaa attcttctct tacacttggt
     3781 actgaaagcc caataataat ctgaatagct tttccattat gcactaaacc atgtgcacct
     3841 actgccttaa aatcttcttc tttagaaata attgagtcat ctttcaaggt taatctcaat
     3901 ctcgtagcac agtttgtaac atctacaaca ttttctgctc caccaactaa ttttatgtat
     3961 tcatatgctt gttgagaggc tttactcaat ttatttcctt tttcatcttt cgtttctaaa
     4021 tattcatttt tagtatatag ttttgtttct tcactatctt cttctctacc aggagttttc
     4081 atatcaaact tcttaattaa gaaagtaaat acaaagaacc agataaatga aaatataatc
     4141 cctacaacaa cttgaacaac atataccttc cagtgataca ttccaagagg aagccaattt
     4201 aatacaaccc agttaataat tcctccacca aaatcaccaa caactcctaa ggcaaacgaa
     4261 atcgtttgca agcatgcatc taaaaatgca tgtactagga ataaaagtgg tgctacaaat
     4321 aaaaatgtaa attctaacgg ctcagtaatt ccagttaata ctgcagtaag agtagcagga
     4381 atcattaaac ctaatacttt tttctttttt tctggctttg cagttacata gaaagcacca
     4441 aatacaccta atgaaccaaa caccttcgat aatccggata atgaaaatcc gattggatac
     4501 atttccttta gagtcttagc tgaagtttga aaatcctgaa tatgagtagc ccaatatact
     4561 gatgtaccac ctggaacaac aacactatca taacaaattg gtgcatatac aaaatgatgt
     4621 aatccagttg gtattaaaat cttttgtaag aatgcataac accaaactcc tatatttcca
     4681 ctatttataa aaaacgtttg caaatgtcta ataacatttt gaatatgagg ccaaataaaa
     4741 caagttaata atgcaactgg tatcataaca aagaaacaaa ttgcaactac aagtgatgag
     4801 cctctaaata atccaataaa atctggcagt tctttatcat aaaatctatt atgaatataa
     4861 atgacaatac ctgatattaa caaagcacca accataccag tatctaatgt tttaatacca
     4921 gcaatgctta caagtccact tccagcactt atttcagctg cgaaatcaac tccaaagctt
     4981 tttccccaaa aacttaacat agaacctaca aaataattaa aagtcaaata cgttgctaaa
     5041 gcctccatac aagctcttgc ttgttgcttt tttgcaagtg ctattggtaa agaaatagca
     5101 aataataatg gcaattgatt aaataccatc caagctccac tagatattac accccaaaat
     5161 tggtaccaaa tattttctgg tgtagcaagt gaacccataa ttgactgatt ttgaaataca
     5221 attgagaatc caaccattat cccagcaaaa gcaaataata gtgttggtgt aaacattgcc
     5281 cctccaaaac gctgtatttt cttcatcata taaattactc ccctcctcat atttttagtt
     5341 tatctttggc cttagatatc tattatttta acaggttaat aaatatatac aatctttagg
     5401 gtacattcgg agcttattca cagtcaaata agtttttgca catttttatg ttcaaattct
     5461 cataaaaaaa aagaatgact acaatttgca atcaatccct tctgtaatca ttctatgcct
     5521 tttataaata ctatatttaa taaataataa ctcaatcaat ataaaatata acattgtcaa
     5581 tttataacta gggtgcattt gtaaaaatga gatattagtc gctgaaatat aaattgattc
     5641 atcacttaaa tcagtcaatg tattattttt aaactctgta attgaaatta ttttagttcc
     5701 tattaatttt aatcttctag caatattaag tgtattctca ttctcaccac ttagggatat
     5761 aataatcata acatcatttt tactgacaat attgtaaaaa gaatcatcaa atgttcttcc
     5821 ctcaaaatca tatattattt cttgacatga taagaacatt ctctttatct gttttgcaac
     5881 cacattttga atatcacctg aaccataagc gtatattgtt cttgattcat aaagaagttt
     5941 acacaatgca tcataatctt tatttttttg atcttcaaca tacttaatta ttgaattaca
     6001 tgcaatatca aatgactgat tttctatgac atgaaccttt tctgattccc agcttaataa
     6061 tactttaaac tcactgaaac cttttaaatc taatttttta acaaatcttg taatagttgc
     6121 acgagacaca ttacatttca tagcaagttc atttattgac atcttactac aatgtttttt
     6181 atttgcagtt atatatttcc atattaataa atctgtatca ttcaatttat tataatttaa
     6241 aaaaatcatt tcttctaatc tcataaacta ttaccccctt taattaaaaa tcgtcataaa
     6301 tattatacca atattcaaca ttaaattaca gcgtattctt atatttacta tttagcccat
     6361 aaaattgact ataaaaaata ctgctataat tgatgtttaa aaaaatgtag ttttaatttt
     6421 attcttttat ctaataatta atattttcaa gttctagtgc ttttcttata caattatcat
     6481 ctttaaaatc agcagtcctt ttacaatctt ttactttaaa ttctggaata gttttaaatt
     6541 cctcatcaca tattccattc ttatttaaat acatacaact tgccattcta actattagac
     6601 cactattttt taaattaaac aatacagggt ctatacaccc tccatctccg ccagtagttt
     6661 caccaactaa agtcgcaaat ttattttctt tacaaaacat tgcaaatgac tctgatgatg
     6721 aatatatttt tctatcaaca agtaagtata tattaccctt aaattttgaa tcatctgtag
     6781 attttatagt ataggaagta tcttcaaaat cattaaaatc ttttataatt tctttaggtg
     6841 cattttcctt tacacttgct ggtaaatttt ctacaggatt taattttata tttcttgcat
     6901 ttgtatattt ttttactatt tcattattat ttctataaat tctataacca tttattttga
     6961 catcattttt tatgagttta gatacgattc cctgccaata cctatcactt ccacctaaat
     7021 tacctcttat atcaataatt aaagctttat gtttttctaa agtgttaata taatctccaa
     7081 ttatttttaa atcattattt atagagccat tctttgatga cattgatggt aaatacatat
     7141 atccaatctg acccttaata acatccttta aaattaattc tttttttaca aaaatatcat
     7201 ctggcatttt tattttaaga ttcatagagt catatctatc cacaactttt ttatcattaa
     7261 gaaaatcata ccaatcatta ttagagtatg atttcttaaa aagttcatat cttttcttat
     7321 tatctattac ttctgtgtgt ttattattta aatcagataa tatagatgac agttcttcta
     7381 taaattcttc atcattttca gtgtttttta ttcgttttgt atattcatcc ttatttgcta
     7441 accaatcaat attattaagc ctcttattta cacctagata tggatatcct gtctttacaa
     7501 cattatacac ataattaaag tcatccacct tttcctcttc agacaactga ttcttttgag
     7561 aataagtaga attacctaat aatattataa atgctaaaat aattactaaa aataatactt
     7621 tctttttcat gtaattctca tccactttca gattttatat tttaatac
//
LOCUS       DAGSHT010000158         7650 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGSHT010000158 DAGSHT010000000
VERSION     DAGSHT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.164"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..756
                     /locus_tag="MJB72_002877"
     CDS             <1..756
                     /locus_tag="MJB72_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBY8263952.1"
                     /translation="ITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEIYKGEKFDTG
                     KLNIVAKDNTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSH
                     DYNSPEFKKMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYI
                     DGKNAPQVFSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANML
                     SDEFYSTGFGLYILSDGQVHATQEFLNGNEGSL"
     gene            975..2573
                     /locus_tag="MJB72_002878"
     CDS             975..2573
                     /locus_tag="MJB72_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY8263953.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            2865..5045
                     /gene="slpA"
                     /locus_tag="MJB72_002879"
     CDS             2865..5045
                     /gene="slpA"
                     /locus_tag="MJB72_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBY8263954.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            5281..7626
                     /gene="secA"
                     /locus_tag="MJB72_002880"
     CDS             5281..7626
                     /gene="secA"
                     /locus_tag="MJB72_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBY8263955.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
BASE COUNT         3063 a          765 c         1533 g         2289 t
ORIGIN      
        1 ataacctctg taggtgaaat agatgagaag gtaatacaac aatgtataag tgcttcaaag
       61 tcaaatggac aacccccagc aattacagtt ggaagtacag agatatataa gggtgaaaag
      121 tttgacactg gcaagttaaa tatagtagct aaagataaca ctgggaaggt attaccaata
      181 gaagttgatg ggtttataga tacaaataga gtaggtacat acatattgac attaaaagct
      241 acagacgaat ggggaaaaag tacaggaaaa agagtagaaa taaaagtgtt ggatgataaa
      301 tctcatgatt acaacagccc agaatttaaa aaaatggtat ctactgaaat gtataactta
      361 atcaattcct atagaaaaga aaaaggtaaa gagcctttgg tagtatctag taagctagaa
      421 ggtatggcaa atgcatggtc taagtatatg atggataaca aagtttttga acattatata
      481 gatggcaaaa atgctccaca agtattttct gagtttggaa tgagaagtga ggaaaatata
      541 gcatatattt atatagattc taagagtgtt caaacaactc aagatgcaaa agacttggca
      601 aaggctatat ttgaagtgtg gaagaaatct ccagaataca atgcaaacat gcttagtgat
      661 gagttttata gtactggatt tgggctttat atattatctg atggtcaagt gcatgctact
      721 caagagtttt taaatggcaa tgaaggtagc ttgtaaaaaa ttccataaat cttgtttttt
      781 tgttaattta ctgatattat aaaatgaaaa tcacatctaa agtacttttt gtaatctgtt
      841 ttattattgc tttacaggtt atggtatttt aggtgttttt tgttttagta gtttataaat
      901 tttgagggaa tcactactga gggtgtaatt tatgattagc tgaattatat aaaaaaatta
      961 gtaggaggag ttcgatgaag gtaaataaga gagtattatc aataggattg acaatatcat
     1021 taataatggc aggagcacca aatatcaatg cactttcgag tatagaaaag atacaaggga
     1081 aagatagata tgagacttct gcattgatag cagataaaca aatatatgat acagtcatat
     1141 tagtaaatac tgataattct atagtagatg gattaagtgc aagtggatta tctggagttg
     1201 taaaagctcc tataatgtta gtgcaaagag ataagatacc aacagatgtt gaaaaaagac
     1261 taaaggatgt taagaatgct tatgttatag gtacagagga tacaataggt aaatctgtag
     1321 aaaatcaact taagaataaa gggattgaag ttaaaagaat tggtggggaa gatagaataa
     1381 agactagcta tcttatagct aaggagattt cagctattaa accagtaaat gatggtgata
     1441 aggtttttct tgtaaatgga tacacaggtg aggctgatgc aatgagtgta tcttctgtag
     1501 ctgctagaga tggagttcct gttatactta ctgatggaaa gagtatacca tttaaggttg
     1561 atggcgttca atgttattct cttgggtcag aggagataat gagtaatgaa cttgtgagta
     1621 agactaattc tgttaggatt gctggtaaag atagatttga aactaataag aaggttatac
     1681 aacgctttta taaaggaaca aagaagtttt atgtttctca agggtataag ttagttgatg
     1741 ctgtggctgg ttctccgttg gctaaggaca aacctatagt acttgttaat gatggaagtg
     1801 ataagagtgt gcttagaggt gctgatgaag ttacttcttt aggtggaatg gataagaagg
     1861 ttgctgacca atgtattagt tctgcatctg ataaaaatac aatgcctaca atcactgcta
     1921 atgatgtaga gatttctgta ggggataagt ttgataatag tatgttgaat atagttgcca
     1981 ctgattatta tggaaataat ttgaagccta atattgaggg aactgtggat accagtaagg
     2041 caggaacgta tgttttaaat gtaagtgctt ttgataattt tgggcaaaag tgtgagatta
     2101 atgtaaatgt caaagtaatt ataaacacaa gtacaaaaga ccctaatagt tatgagttta
     2161 aggctatggt atctaatgaa atgtataatc tagttaattc atatagaaaa gaaaaaggta
     2221 agaaaagttt aagagaatta gattctttag ctggaatggc aaatgcatgg tctaagtata
     2281 tggatgagaa gaagatattt gctcatgaaa tagatgggaa aaatgcagct gaagtatttt
     2341 ctggctttgg aatgagaact gatgaaaata ttgcatattt acctatgaat gttaagtctg
     2401 tatatacatc taaagatgca aaagagattg ctaagtctgt atttgattta tggaagaagt
     2461 cttctaagta taatgataat atgttaaagg aagagtttta tagttttggc tttggaatgt
     2521 atgtttctaa tcaaagagaa gttaatgcta caatggaatt tttaaatagt tagagtatat
     2581 attaatattt tatggtagaa aatattaata tatattgatt aaatcagata aataaatgta
     2641 ttaataaaat taaaattgaa aatttaattt aaaaaatatg aaatagcaaa aaaaggggaa
     2701 aaatgttgaa agaaaattct attaattttg cataaattat tgaaattcta ttttatatag
     2761 tctatcataa gagtgtgaga aatatttggt ttatatgaac atggaatttt aatgtacata
     2821 attaaagaga tgtaattaat ataatgttgg gaggaattta aggaatgaat aagaaaaatt
     2881 tggcagttgt aatgtctgcc gttacagtaa tcggatcagc agcaccagtt ttcgctgcag
     2941 cagaggatag ttttccaaat ggaaatacca ctgtatctag tagtaagtat agtgatatag
     3001 aaagaatact taaaaaatat gttgcagatg gtgtaacagg cataacagtt aacttttttg
     3061 acaaaaaagg aaatccaaaa ggggtatcta tatcaagttt agaagcaagt caaataagtt
     3121 atgttaaaga tgaaattaaa gatttaaaag ctggagaata tgcaaaaata tctgtaaaaa
     3181 ttacagatgg aacagaaaaa aaatatgatg atacattatt aaaggaactt gatagtaatg
     3241 ctgtagttac taaagttggt actgcaacaa cagttaagat tgtagatcaa gatggaaatt
     3301 caatagaaaa tgatgctaaa ttaaagttac aaaaatatga tacaactacg tcagcttatg
     3361 tagatggagc tactaaagat gaagttttag cagctacaaa ggcagcagtc aaaacttcaa
     3421 aatttactga taagactggt aataatgcta caggaacagt agctattaca gcaacatatg
     3481 ctgatgcagt agctggtgca attaaattaa aatatgatac acaagcagca gtagcaccta
     3541 ctttagatac agcagtagca tctcttgatg gtactaaaga gatatatgat tttagtaaac
     3601 cagttataac ggttaatact actgatggta catcaaaatt aagttttgaa aaaactggag
     3661 aaaaagttgg agcagttgaa tcaaatgtaa cagttatagc agcatcagat gaaacagtta
     3721 caatatctgg agatgcaaaa gaaaaagcag aagctttagc taaaaaatat gtatttaaag
     3781 atactgaatt agaagatgca tataagacag taacagcaag tgattttgaa aaaactgaca
     3841 atgattatta tgaggtagtt ctttatccaa caggtaaaag attaaataca gcttcaacat
     3901 atgcatcatc taactataag ccagaattac ctacaagtga tagagtagat actcctgcta
     3961 taataacatt aagatctact aataagaata acttaaaatc agctttagat gaattaagaa
     4021 cttataataa ttcttattca aataattctg tattagctgg tgatgataga atagagactg
     4081 caatagaaat aagtaaagac tcttataatg cagatggagg aattaaagga gattatgtag
     4141 aagctaatga ggtagtatta gtaggatcac aatcaatagt agatggtcta gtagcttcac
     4201 ctttagctgc tgttaaagat gctccattat tattaacttc aaaagataag ttagattcat
     4261 cagtaaaatc tgaaataaag agagtaatgg gattagatga taaaactggt ataacatcta
     4321 aaaagactgt ttatatagct ggtggggaaa attctgtatc taaagaagta gcaaatgaat
     4381 taaaagatat gggattaaaa gttgaaagat tatctggtga tgatagatat gctacatctt
     4441 tagaaattgc tgatgaaata ggtttaaacc ataataaagt atttgtagtt ggtggtactg
     4501 gattagcaga tgcaatgagt atagcatcag ttgcttctaa taaagaaatg ccaatagtag
     4561 tagttgatgg aaaaggaaaa gatttatcaa ctgatgcaaa agactttata ggaagtgctt
     4621 atgtagatat aataggtgga aaatctagtg tttctgaaga catggaagat gctatagatg
     4681 atgctacagg taagagtcca gaaagagtta gtggagatga tagacaagat actaatgcag
     4741 aagttataaa aacttatttt gaaaaagata atagtgacag tgtaattagc acaggtgtta
     4801 aaaacttcta tgttgctaaa gatggttcta caaaagaaga tcaattagta gatgcgttag
     4861 caatagcagc agtagcaggt cacaatgaag ctccaatagt tttagcaact gatagtttat
     4921 cttctgacca atcagtagca ataagtaaag ttacaaattc tgatgattct aagaaattga
     4981 ctcaagttgg taaaggaata gcagattcag ttattaagag aataaaagat ttattagaat
     5041 tataagataa aattaaacta taaataagag ggaacttctt tgaagaagtt ctctttttta
     5101 taaaaagaaa ttaaaaaagg aactaaaaat agttcctttt tttgtgagaa atacctaaat
     5161 aattaaagat gtaatttatt taagtatgat tattatacta taaataaaaa taattgtcta
     5221 ctattctata attttaaggt ataatagtaa ttgtatgata aaaaaggagg taaattaaat
     5281 atgtcagttt tggatacaat attggataaa gcagatgagc aggaaataag aaggcttaat
     5341 gtgatagttg ataaaataga atctttagaa aaagatatag aaaatatgtc tgatgacgaa
     5401 ttaaaaaaaa tgacagacct atttagagat agattaaaaa aaggagaaac tttagatagt
     5461 ttattaccag aagcatttgc agtggcaaga gaagcttcaa aaagagtttt aggaatgcgt
     5521 caatataggg ttcaattaat tggaggcatt gtaattcatc aaggaaaaat agcagaaatg
     5581 aaaactggtg aaggtaaaac tttggtagaa gttgcacctg tttatctaaa tgctttaaca
     5641 ggtaaaggtg tgcatgttgt tacagtaaat gactatctag cacaacgtga taaagagcta
     5701 atgcaaccag tgtatgagtt tttaggtatg tctgtaggag taattattgc agatcttata
     5761 cctgaagtta gaaaagttca atacaattgt gatattactt atggtacaaa taatgaattt
     5821 ggatttgatt atttaaaaga taatatggta actaaaaagt ttgataaagt acaaagagag
     5881 ttgaattttg ccatagttga tgaggttgac tcaattctta tagatgaagc tagaactcca
     5941 cttataattg ctggagaggg tgatgaagat tcaaaacttt atgaattagc aaattcattt
     6001 ataaaaacaa taaaagaaga agattatgaa ttagatagaa aagataagac cgtagctcta
     6061 aaggaaagtg gtattgataa ggcagaatca ttttttggga ttaaaaatct tacagatata
     6121 gaaaatatag aattatatca tcatataaat caggctctta ggggtcacaa acttatggaa
     6181 aaagatgttg attatgttgt tgttaatcaa gaagtaatga tagttgatga atttacagga
     6241 agagtaatgg atggaagaag atatacagat ggcttacacc aagctataga agcaaaagaa
     6301 ggggtagaaa taaaagatga atctaaaact atggctacaa ttacattcca gaatttcttt
     6361 agattatata ataaactttc tggtatgaca gggactgcta aaactgaaga aggagaattt
     6421 gaatcaattt ataaattaaa tgttgtacaa attcctacta acaaaccaat tataagagca
     6481 gatcttcatg ataaagtgtt tagaacagag aaagaaaagt atcaagctgt tgtagaagaa
     6541 gtaataagga tacataaaac aaaacagcca attcttattg gtactgtttc tgttgagaaa
     6601 tcagaaatgt tatctggtat gcttaagaaa gaaggaataa aacatcaagt tctaaatgct
     6661 aaggaaaatg ataaagaagc attgatagtg tcaaaggctg gtaagttggg ttcagtaact
     6721 attgcaacta atatggcagg tagaggaaca gatattgctt taggagcagg tgataaagaa
     6781 gaagaaagta gagtaaagga attaggagga ctttatgtaa taggaactga gagacatgaa
     6841 tctagaagaa tcgataatca gcttagaggg cgttctggac gtcaaggtga tccaggaaca
     6901 tcaaggtttt ttgttagtct tgaagatgat gtaatcaaat tgtatggtgg aaaaagtata
     6961 gagaaactaa tgaaacgacc aaattcaaat aaaaataatt ctattgaaag taaatctctt
     7021 acaaaggcaa tagagagagc tcaaaaaggg gtggaaggta aaaattttga attaaggaaa
     7081 aatgttctta aatatgatga tactataaat gaacaaagaa aagtaatcta taacgaaaga
     7141 aataaagttc taaatgatga agatatacaa gtagatattc aaaacatggt taaagatata
     7201 ataaaagaag ctgctgctga atatttaaca aaaagaaaga gggattacta tggatatttt
     7261 aagcatcttt ataatacatt tatgccagct gataccttac taatacctga cttagataat
     7321 aaaagtattg aggatataat tgatagtact tatgaaatat caaaaagagt ttatgattta
     7381 aaaaagatgg tagtaggcct tgacaaaata accgaaatgg aaaaaattgt tcttttaaaa
     7441 gtagttgatc aatattgggt agaacatata gatgctatgg agcaattaaa acaatatgta
     7501 ggatttaaat catatgctca aaaagatcct ttcaaagaat atgctatgga aggttatgag
     7561 atgtttgaat cattaaataa aaatataaga gagacgacta tacagtattt atataaattt
     7621 aattaagaaa tttaaactgt ataggaaaaa
//
LOCUS       DAGSHT010000159         7613 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.165, whole genome shotgun sequence.
ACCESSION   DAGSHT010000159 DAGSHT010000000
VERSION     DAGSHT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.165"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            335..1624
                     /locus_tag="MJB72_002881"
     CDS             335..1624
                     /locus_tag="MJB72_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBY8263956.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1678..2757
                     /locus_tag="MJB72_002882"
     CDS             1678..2757
                     /locus_tag="MJB72_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBY8263957.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2778..4322
                     /locus_tag="MJB72_002883"
     CDS             2778..4322
                     /locus_tag="MJB72_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBY8263958.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4485..5027)
                     /locus_tag="MJB72_002884"
     CDS             complement(4485..5027)
                     /locus_tag="MJB72_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBY8263959.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5175..5459)
                     /locus_tag="MJB72_002885"
     CDS             complement(5175..5459)
                     /locus_tag="MJB72_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY8263960.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5522..6286)
                     /locus_tag="MJB72_002886"
     CDS             complement(5522..6286)
                     /locus_tag="MJB72_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBY8263961.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6568..6834)
                     /locus_tag="MJB72_002887"
     CDS             complement(6568..6834)
                     /locus_tag="MJB72_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY8263962.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(6876..>7613)
                     /locus_tag="MJB72_002888"
     CDS             complement(6876..>7613)
                     /locus_tag="MJB72_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8263963.1"
                     /translation="RQYIAHYTISIPDTSIKENLTLIPDASGCMIFKFDKKGIESAFW
                     GATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNTLFSLEV
                     NSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKNISQISC
                     YSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEIQNNKIPFATLAQDIGYYDQSHFIN
                     DFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2631 a          978 c         1255 g         2749 t
ORIGIN      
        1 cagagagtta gatttggctg agaactaata ttggaattta tgtgaaaagg actttggaga
       61 atactattgt aattatgtta gaatagtggt ttaattcgcc ttaagaaaat gagtgagtaa
      121 aattaatttt tactaataag agtggtaaca cggatttata ttcgtctctt ggatttttcc
      181 aagaggcgtt ttttattata aacttatttc agaaaattta aaactatata ttaaatgaaa
      241 attataaaaa tatgatagtt aataaattta taaattagaa aattgtaaaa tatgctaagg
      301 tcagtagttt tacgatttaa atggagggat gaaaatggaa gaagaaaaaa ttaactatgc
      361 tctggaacag attccagaga atcaaaaacg tggttgggta gctatgtttt cagttttagt
      421 agcaataggt gtggatttat catctgttat tttaggcgct gagttggcac agagtatgcc
      481 aatgaaacaa gcaatattgt cagtaattgt tggttcattt ttttctgcaa ttttgtatac
      541 tacatgctct ttggttggtt catctacaag tttatctaca tctatgataa caaagtatgt
      601 atttggagaa gctggagcaa aaatattttc tctagttata ggggtttctc tattaggttg
      661 gtttggagtt caagtaggtt tctttgctca aaatgcacag ataataataa aagatatatt
      721 taatttagat gtcagtatgc agattttaag cctaataggt ggtctcttga tgatgagtac
      781 tgcaatttat ggatataagg caatggagaa gttgagtgtg tattcagtac catttttatt
      841 agttcttatg atgctaacta tattcttagc ttttagagca aatggaattt cagttgatga
      901 taatatgaaa tctactatga catttgcagg tggagtttct ctgtcaatga gtataataat
      961 agttggtgca attgtatctc ctgatataag tcgttgggct aaaagtagaa gagactgtgc
     1021 tctttcaagc tttttgggaa tacaatttgg aaatgctttt atgataatag tttcgatagt
     1081 attagtaaaa tgtatgggaa cttcagatat tatgagaatt tttattacat taggaattgc
     1141 tataccaggt ataatagtac ttacattagc acaatggact acaaatacaa gtaatgtata
     1201 ttcagcatca ttaagtatag ctcttgtact taagaaggct cctgagaaag tattaactat
     1261 agtccttggt attatagcaa cattattggc tgtatttgga atatatgaag gttttatagg
     1321 atttttaaat ttacttggaa ttgtaattgc acctgttggt ggtgtatata cagcagaata
     1381 ttacatagta aagcaagaac ttaaaggctt tgataaagga gtattatata aaccaatagt
     1441 taaacgttca atagtgtctt ggataattgg tatattaata acttaccttt caacatatgg
     1501 atttataact cttactacta tagcaccttt agatggtttt atagcagggt ttgtagtaca
     1561 atctattatt ggaaaagtgc tttgttcaac aaaaaataag caagaaatag acaaagcagt
     1621 ataaagaaat ttgtttataa agtataaaat aaattagaaa tatgaggaga aagtaacatg
     1681 agatttttaa cagaagaaag tgtagataaa atagcagttg gtgcagcagt gcttggaact
     1741 ggaggtggag gtgacccata tgtaggtaaa ttggttgcaa aacaagccat taaaaagtat
     1801 ggtcctgtaa aagtaataag tttagatgaa cttgatgatg atgctttggt agttccagta
     1861 tctggtatgg gttctcctgt aattacaata gaaaaattac tttcagaagt agagttaact
     1921 acccctttag aaataatgga gaaattatta aatagaaaag tggatgttat aattcctata
     1981 gaaataggag ggataaactc acttatgcct atagcagttg ctgctaaaaa aggacttcct
     2041 atcttagatg cagattctat gggtagagct ttccctgaag ctcaaatggt aactttttat
     2101 cttgaaggat atgaggcatc tcctgccgta atgtctgatg aaaaaggtaa ttctgccatc
     2161 ctttatcctg tggatggaat ttggtcagaa agattagcta gaactttaac tgtagaaatg
     2221 ggaggaagtt cttctattag tgactataat ttaagtggag ctcaagttaa aaaagctgct
     2281 ataggtaata ctcttacaat agctgaaact ataggagggt ttttacttga aaataaatct
     2341 gattcaaaag gtgctgttga gaatatacta aaagaattgt gtggatacaa gttatttgaa
     2401 ggaaaagtaa ttgatattaa gagagagttg aaaggtggat ttactagagg acatgccttt
     2461 tttgctggga ttaatgagta tgatggagag tattctatat tattccaaaa tgagaattta
     2521 attgccaaaa aaggtgatac accactttgt attacaccag atttaatagc agtattagat
     2581 ttagaaactg gatttccaat aactacagag agaataaaat atggctctcg tgttatggta
     2641 gtagcttttc cttgtaatga aaaatggaga acagaaaaag gaattgagac agttggacct
     2701 ggatactttg gatatgatgt ggaatataaa acagtagaag aattacaagg aaaataaaag
     2761 tttaggagga aattttgatg tatcgtgtag gaattgacgt tggaggaaca aatacagatg
     2821 cttgtatact ggatggggaa ttaaaagtaa ttcattcagt taaggtggca actacaaaag
     2881 atgttgaaac tggtgtttac aatgccttaa aaaaagttat agatgaaagt aagattgacc
     2941 attcattaat aaaatatgct atgcttggta caactcattg tacaaatgct attgtagaaa
     3001 gaaaaagact taataatgtg gctatgataa gaataggaaa gccggccact actgccattc
     3061 ttccatttat agattggcca gaagacttaa gagaaaaaat agaattagat tcaatattgg
     3121 tatctggtgg atatgagttt gatggcagaa aaataaatga actttgtaaa gatgaagttg
     3181 tagagttttg caatagaata aaaggcaaag tggaaagtgt tgcaataagt ggagtatttg
     3241 ctccagtaaa taaacaacaa gaagaagaag ttgcaaaatg ggttagagaa attttagggg
     3301 atattcctat atctttatca aatgaaatag gtaatatagg tatattagaa agagaaaatg
     3361 gagctatttt aaattctgca ttgtctcaag taggtaaagc tgtatcttta ggatttgaaa
     3421 aagcacttaa agatttaggt atagatgctc aaatatattt cagtcaaaat gatggaacat
     3481 taatgaattt agagtataca atgaaatacc caatttttac aattggatgt ggaataacaa
     3541 attctataag aggagcttcc ttcttatcaa aagtaaaaaa tgcaatagta ttagatgttg
     3601 gtggaactac aagcgacttg ggtgtactat ataatggatt ccctagagaa tcatctattc
     3661 cagttacaat tggaggagta catacaaact ttagaatgcc agatgtttta actattggtc
     3721 ttgctggtgg tacatgtgta aaaggcgaaa aagataatat aaagataggt cctgaaagtg
     3781 tgggatatag aataactgaa gatgcaataa tatttggtgg agacactcta actttatcag
     3841 atgttgttac aaaattagat atggcgtttg aagatagaaa aagcaatgtt gaaaacttag
     3901 ataaagagta ttgtaatgag gtatataaga aggttatttt aaaattagaa gatgctgtag
     3961 accaaatgaa gactagtcaa gatgatgttg aattggtttt aactggtgga ggaagtataa
     4021 ttgttcctga aaaactaaaa ggcataagca atgttataag acctcctcat tatactgctg
     4081 caaatgctat tggtgcagct ttaggacaaa taagtggaga tgtagaaaaa gtatacagtt
     4141 tagataaaat gtcgagagaa gatgccatag aagatgcaaa aaaagaggct gtaaataaag
     4201 ctctaaaagc tggtgctaaa gaagatacta tagaagtatt aaatatagaa gatgttccac
     4261 ttgcatacct tcctggtaat gcacttctta taaaagtaaa agttgttggt gatttaattt
     4321 aaagaataaa tataagtatt tccagaatta tataaatatt tctggactca tttttaaact
     4381 tattttatca ataaaaacag cataactttt gactatattc ttataagttt ccgaacagcc
     4441 gatatatacc tattttttga ggtttatcgg ctgtttgtta tcttttatcg cattccaact
     4501 atttttctta ccgtatttgc tgtacgaatt gtaatcttat cacttacatt tgaactggca
     4561 gtctttgacc accaatttgt ctttttataa tctttacgac taaaagacca aaagatagca
     4621 tttttataat gatatacttt ttcatactct tctttaggat ttccaacctc atcataaaac
     4681 tcgtcataag ataagtcagg aaacataaaa ataagattat catatatttc tttgttgtta
     4741 tctccccacc aattaggagc attatttagt atatctttta actcttcttg tgaaataata
     4801 aaaactggta tttccaattc aaactgtttt tttatcatta atcttattgt atttgagata
     4861 accttgctgt catctacatc acttaaaaag acaacattac cactattaag gtatgtagaa
     4921 acttctgaat aaccaagttc tacaaagctt gcttttaatt cagacataat tatcttgttc
     4981 ttaccactta tattgatacc tcttaataat gcgatataac ttttcatata ataatttcac
     5041 ctctaaacat tcacattttt ctatgtagta caaacttgaa tttgcatctt ttataattct
     5101 ttcaaaactt cttcatatgt aattttttta atataaaaaa atcggtgctt aatttgtacc
     5161 gatttttgtt tttcctagta aagaactcgt ttttttgctt ctgctatagc tctagcagca
     5221 ctttttgctt gcattctttt ttcttcttct tttttaaatt catttattgt ttcatttaga
     5281 ttcttatcat gttcattttt ttctttatct tcgttttctc tgtatactaa tatataatct
     5341 attattgatt cgtttattat ctgagatata cttttatttt cattaattcc tattctctta
     5401 acttccttaa gtagagtctc atcaatatga accgttgttt gctttgtatt ttttgccacc
     5461 ttaatcacct cttaaaaaaa attctaacat taaaattttt tttagtcaat aaattttaat
     5521 attaaaattc tttaaatgtt ttatttgatt ttctaaaatt ttctattgta tttatcatct
     5581 tattttttat attttcacct agtacagaag actttagtct aggattttcg atatggtcat
     5641 aaattggttc taaaaattca acttcgacag gaacagagtt aattttacca ataggttggt
     5701 atccttcata agtatctttt gaatttttta ttacgagtgg aacaataggg cattttgctt
     5761 tatatgctat ctttaaagct ccacttctaa actccgaaac caatgaattt ggttctttaa
     5821 tccatgttaa atctccttct ggaaacactg ccatactttg acctgtaagt atattttgag
     5881 aggcttgggc tatactcttt ataccctcac gattgttttt tctgtttaca tatacacatc
     5941 ttagtaattt tgcccattct ttaaatatag gtatatttct ccaaactggt tcatctgcaa
     6001 ttacacatcc aattggtctt tctacacttg ctgttagtat gaagctgtca agcatactag
     6061 aatgatttac aacaaataga agtggttctt ttggcagtgt ttccttacca attatattaa
     6121 gctctatatt cactatatct aaagattttt tagcttgttt ttgcataaat tcaaattttt
     6181 cctttaagct aaatttatca ggattctttt taattttaat aagcctaggt atagaagatg
     6241 taaaacctat aagttgatat gtagcaaatt ttaagtaatt attcataaaa ttatctcctt
     6301 aaaataaaat tatttattac tgtaaagatt ttaacataaa ataaaattta agtctatttt
     6361 aattcaattt tgtaacatat attttaaata gacagagatt gagattttat atttccaatt
     6421 ttagtaatca gattttatca ataaattact attctattaa tttaattaaa taacctttaa
     6481 atttaattat aaaaactgtt gcagaaattt ctacaacagt ttaaaaaatt taccttacaa
     6541 aaacttattt tataacaaga gagtatatta taaggctagt acagcccatt ttttgcactg
     6601 agaaattctt ttgccatcat caccttgttc tttgtcatat gcaaattgat taagcatttc
     6661 acatggaaat gtatcacata gaccacaatg tgtaaggttt ttatcttcac aacaagattt
     6721 tacaggacat tgttcacccc aaaatggttt ctcaatctct gtacaacctt tacaattgac
     6781 tttttccttg tatccacatt cataacataa aattccacat cttgattcta tcattttaag
     6841 tcactcctta aatataaatt ttatatgatt gtatatcaaa atttatactt ttcattgtaa
     6901 aaatcggaca aattttttat ataggttgtt ggattaacac cacaaataga tttaaaatca
     6961 tttataaaat gagattggtc gtaataacca atatcttgtg ctaaagttgc aaatggtatt
     7021 ttattatttt gtatttcttg taatacaagg tttatacgaa gtaatctaag gtatgattta
     7081 acactcatac caagagaatt attaaaaatt ctatttaaat gtcgttcact ataacaacta
     7141 atttgtgaaa tgtttttaac agacataata gagttttgtt ttctagcatt ttcaagaatt
     7201 ggtatagtca tatctactat atttgatttc aataagtatg ataagaataa catatctaat
     7261 tgttctacta attcctttat agtacttgtt ctttcaaaaa tggaattaac ctctaagcta
     7321 aataatgtgt taaaatcttc aagtgaaatt tttaaatctg tagattctct ttgagaaaga
     7381 cctgtcaaat aatatactcc acctgggcga aactcaacga atactctaaa aagtacattt
     7441 tctatatcat tttttacaat agtagttttt gttgtggctc cccagaatgc gctttctata
     7501 ccttttttgt caaatttaaa tatcatacat ccactagcat caggtattaa tgtaagattt
     7561 tctttaatac ttgtatctgg aatagatatt gtatagtgtg ctatatactg tct
//
LOCUS       DAGSHT010000160         7598 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.166, whole genome shotgun sequence.
ACCESSION   DAGSHT010000160 DAGSHT010000000
VERSION     DAGSHT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.166"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..895)
                     /locus_tag="MJB72_002889"
     CDS             complement(<1..895)
                     /locus_tag="MJB72_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY8263964.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLENCF
                     NKFKESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKL
                     KDIEEGYFYLLEDVYFDMKDVL"
     gene            complement(934..1794)
                     /locus_tag="MJB72_002890"
     CDS             complement(934..1794)
                     /locus_tag="MJB72_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBY8263965.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2063..2272
                     /locus_tag="MJB72_002891"
     CDS             2063..2272
                     /locus_tag="MJB72_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBY8263966.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2272..2538
                     /locus_tag="MJB72_002892"
     CDS             2272..2538
                     /locus_tag="MJB72_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBY8263967.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2567..3139
                     /locus_tag="MJB72_002893"
     CDS             2567..3139
                     /locus_tag="MJB72_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY8263968.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3374..4564)
                     /locus_tag="MJB72_002894"
     CDS             complement(3374..4564)
                     /locus_tag="MJB72_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY8263969.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4824..5255)
                     /gene="dut"
                     /locus_tag="MJB72_002895"
     CDS             complement(4824..5255)
                     /gene="dut"
                     /locus_tag="MJB72_002895"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBY8263970.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5368..7596
                     /locus_tag="MJB72_002896"
     CDS             5368..7596
                     /locus_tag="MJB72_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBY8263971.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
BASE COUNT         2657 a         1214 c          975 g         2752 t
ORIGIN      
        1 ctagtacatc tttcatgtcg aagtacacat cttctaataa ataaaaatag ccttcctcta
       61 tatctttcaa tttatctata gtatccattt ttttacttat atctttcata atttctaaat
      121 tttgtatatt tacatctatg attgttaata agtacattct cacagttttt attatttcaa
      181 cagaaaaatc tacaatatta taatttcctt cttttttata taatgactcc ttaaatttgt
      241 taaaacaatt ctctaaatta gaatttatat cgttaaatgg tggtcttatt atgtcacgta
      301 ttattaaaag attttgtata tctacattat ttgtgattgc ctcatccaaa ataaataaat
      361 tatatgcatc atttaatggg tcatgggcat ctccactaaa ctctaagtta acaaattcaa
      421 gtaaattttt caatgatata tagttcatac atctcatacc acaatttata tatttttctt
      481 ttatatctac aaataaatct ctaatattat ttacaaacct tggatggtta ttttttttag
      541 cacttctcat atctgtgtta ttgaaacatg tcaaatccaa attaccaaaa gtatatattc
      601 ctttaatatc agaaaatatg ccaagccatt ttttaaactt tctcatgacc tcttcataac
      661 ttggtgcact cttcaattct tcagaagata tatgtgttaa ttcttgtata tgaggataca
      721 actcctcatt acttactggt cttattaaag atttaaattt atcaatattt tttgtttttt
      781 catcatacat aactgctccg attgcaatta tatcagaatt aaatactgct ctttttgatt
      841 tagaatttgg catattcatt tcaaaatcta tatatactct tttcaatata aacacccttt
      901 ccaattgttt atagtgattc tttttaacta tttttattca aaattttcta agaaagaatg
      961 tttctcccca tctttttgcc aaccaagtac tgaatcaaga tttacatttt ctgccgcctt
     1021 aaatatagat ttttctacat ttttaaaatt tgattctaag ctctttatta agtcttttat
     1081 ctcatatctt ttattacctg tttttttagc agcaaccata catgcacaat ttaatggcca
     1141 tataccacta ttttgaataa atcttataat atgctcttct cttatataat aaagtggtct
     1201 tataattttt ataccttcaa aattagtaga attaagttta ggaagcattg ttttaaaatt
     1261 tccagcacac aataagttaa gcattgttgt ctctattaca tcatcataat gatgacctaa
     1321 tgcaagtttg ttacacccta attcttctgc ctttgaatat aatgctcctc tcctcattct
     1381 tgcacacata tagcatggat aatcttttgc tatttcatct gctatctcaa atatccttga
     1441 atcaaataaa tgtattggta tatttagata ctcacaatta tctataagta attgtcttat
     1501 atttgcatga tatccagggt ccatagcaat aaattcaaca tcaaaattaa cttgtccatg
     1561 tttttttaac tcttgaaaca ttttagccat aagaatactg tcttttccac cagaaatcgc
     1621 tacagctatc ttatcacctt cttcaaccaa tttataatct cttattgcct tcataaactt
     1681 agaccataaa ttttttctat atttttttat tatacttttt tctatatctc ttactggaag
     1741 tctttcctca aaaggaacta taatttcaca gccttttcct gatagttcac tcatctaatc
     1801 acctcgtata tttatgttaa taatatttta caaaaaagtc taccttcatc cacaaaatac
     1861 tataattttt actttaattt tactaaataa tttatagttt acaataacac ataaacttat
     1921 aataacataa ataacctctt tcttttatta tcttttttat ttttctcatt taatatttta
     1981 ttattagtaa cgatttttaa atgattgaat attatacact agatagagtt acctatctat
     2041 ttacaacaag gaggttttca tcttggaaaa taagactaga gatatcaaaa aaaattgtgg
     2101 tccatctttg gctagaccat acattaataa tcaaattttc acagaaatgt actgcctttc
     2161 tgatgcatta aaatttggaa ctatatttcc agaactaaac ttactagagt ctgaaatgta
     2221 caacaaagaa ttatatgcta agccaaaaaa atatagagga ggtagaagat aatgaaagac
     2281 tcatctagag ctgaacttat gagaaaagtt caggaaacat cttttgcatg tgttgatatg
     2341 aacttatatt tagacaatca ccctgatgat aaaaatgcta tcaacactta taattcatta
     2401 tgtaatcaat ttgcgcaagc tagatatgct tacgaaaata aatacggtcc tttaactaac
     2461 tttgggtatg ctccaagtag atgcccttgg caatgggctg accaaccttg gccttgggac
     2521 agagaattta attattagac atcaagtatt ggaggattga aaattcatgt ggatatatca
     2581 gaaaactata caacacccag ttaatataaa aacttgtgac cctagaatgg ctaaatttct
     2641 tataactcaa tttggtgggc caaatggaga acttgctgca tctttaagat atttaagcca
     2701 aagatataca atgcctactg gtaatatgcg tgcactttta acagatattg gtacagaaga
     2761 actagctcac gttgagctta tatgtactat ggtttatcag ttaacttctg atgcaagccc
     2821 agaagagtta aaagctgcag gtcttggttc aaactatgct caaaatggat atggaattta
     2881 tccaacagat tcaaatggtg ttccatttga tgtaagacct atagcagtta tgtcaaatcc
     2941 cgtaaccgat ttacatgagg atatggcagc tgaacaaaaa gcacttgcaa cttattatca
     3001 acttataaac ctaacagatg acgttgatgt tatagatgta ttaaaattct tgggtcaaag
     3061 agaaataatt cactatcaaa gatttggtga agctttaatg gatgcttacg agttagaaga
     3121 atctcaaaaa atgttctaaa atttttattt gtataactat tattatttga taatttataa
     3181 tttactgttg tgttaaaatt tataaaaaat ataaatataa tttattttct ataaacagag
     3241 aaaataaata aaaagcaggt atcaatcaaa tagtaacatg tattatacta tacattacta
     3301 tcaatattgt acctgctttc atattataag caataaagaa aaatctattt attaggtttt
     3361 ataataaaaa caattataaa actcttcttg catttacata tctacttgaa tagtagctac
     3421 tatttatatt agatattgtt actttttttg atgaacctga agatgcatgt atcatatctc
     3481 catttcctat gtatattcca acatgactta cagaaccatt atttgaacct tgtgtatcaa
     3541 agaatactaa atctccactt ctaaggtctc ctctatttac atatgttcca tacttacttt
     3601 ggtctctaga tactcttggt atactaactc ctacagattt tttcataacg tactgtgtga
     3661 atccagaaca gtcaaaacta tttggacctt ctgctcccca tacatatggt tttccaagta
     3721 gtgtctttgc aaaacttatt acactatcag ctccttgact tggagaatta gtcgaataag
     3781 atggaattga agtatcaaca gaaccttttt cagcaaggta ttgacttgac acatatccag
     3841 tttgattgcc ataacttact tttgtccatc ctccactttc agaaatactt ccaacctcag
     3901 taccataact taatgtagct attttagaat gacttgtacc tggtcctgtt ctaacgttca
     3961 atgactttgc tgttacaacc tttgtaccct ttacaaggtc atttgaactg ttttcagcat
     4021 tttcatttgt agagtcattt acactagttc ctaaatattt acttgaaaca taacctactc
     4081 taccattaga acttattttt gaccatccat ttgactcaga tatgtatccg acttcagtcc
     4141 catatcctag tgttgatatt ttagaactac ttgtacttgg tcctgttcta aaatttaatc
     4201 cttttgctgt tacaactttt gtcgacttaa ctgaagtatc actactttca ttgctacttc
     4261 caaggctatt tattgctaca taatttccat gcacataacc tgtgtttcca ttatatttaa
     4321 cttttaccca agaaccttct tttcctacgt actcaacttt atcacctttg taaagtttcc
     4381 ccattgattc atggtttgta cctggtcctt ttctaaaatt taccgcattc cctgttattg
     4441 ttctgctttc tatactcaca ctctctactt cagactcatt agcatatgct aaattcccca
     4501 tagtaggcat tattatcgca gttgccatta tactggctgc tattctcttt tgattaaaat
     4561 tcataacttt ccactcatct cctcttttct actatcttta tttcacttca aaatataaaa
     4621 gaaataattg acactattaa aatatcaatt atttctttta tattaatatt attacaaatt
     4681 tgtaactatt tgtctatagt aatatttgct taccataaat taccatgtat aaattttaat
     4741 ctttaatagc ctgtaattta gtaatatagc catcttttac cttttttaaa aatggctata
     4801 ttttttaact tttttgtaac tttttaaaat ccagttgagc caaacccacc ttttcctctt
     4861 tcggagtcac ttaattcttt tacttcttct atgttaatat catagatagg ttttactatc
     4921 atttgagcaa ttttcatatg cttttcaacc ttaaaatcat ttttaccatg atttattaat
     4981 ataattttaa gctctcctct gtatccttcg tcaatagttc ctggtgtatt taatacagta
     5041 acagaatgct ttaaggcaag cccacttcta ggtcttacct gcgcttctgt catagtagga
     5101 agctctatac atatcccagt tttgataagt ttagtctctc caggaggtat tacaacctct
     5161 tctatagaat acaaatccat accagcatca cctttgtgag caaaattagg tattatagca
     5221 tcatcattta atttttttac ttttaaattg tacatcttat atctcctttc aactttctat
     5281 aaatattata acttaaattt cgacctcttg aagtttttta tttttttgtg gtaatataat
     5341 tattagtgtt ttaaaggagg attttttatg aaacttgtac ttgctgagaa accatctgtg
     5401 gcaaaaacta tagcatcatt tttaggtgca aagaccagac aagatggtta ctttgaagga
     5461 aatgattata taattactta tgcagttgga cacttagtat cattatatga tatgaaggat
     5521 tatgataaag ataaatactc tggctcttgg aaaatgaaca atttcccatt tgttcctgag
     5581 gataagttta aatttaagat tgatagttct aaaactaaac aattcaacac tgtaaaaaag
     5641 cttttgaata gaaaagatgt agaatatgta ataaatgcca ctgacaatga ccgagaagga
     5701 gaacttatag catttttaat atttttactg gcaaaaaata aaaaaccagt aaaaagaatc
     5761 ttggtaaatg aatggacacc agaagatatt acaagaggta taaaaaacct aaaagatgaa
     5821 gatgaaatga gaaatcttca agctgctgga tatactaggc ttattactga ctggttgatt
     5881 ggaataaatt tcacttctgt tgctacattg aaatatggaa atgggaaact attaaacata
     5941 ggtagggtaa ttcttccaac agttaaatta gtctatgata gagatatgga aatactaaac
     6001 tttgttccta aaacctatta tgaaatagaa gggcatttta aagctgaagc tggtgaatat
     6061 aaaggtaaat atgttaaggg taaagaaagt aaatttgata cacttgaaga tgcaaataaa
     6121 attatagctt caattacatc tgaaactggt aaaatactag ataaaaaagt tactatgtca
     6181 aaggaatatg caccaaaact ttttagtttg acatcacttc aaggatatat aacttctaag
     6241 tattccaatt ttacatctga taaggtttta aatgtctgtc aatctctcta tgagggtaat
     6301 ggtaagggtg gatacataac ttaccctaga actgattcca ttttcttaga agaaagtttg
     6361 gcttcaaaag cttctcaaac tttagataaa ttaaaggttg gtcttgaata tgaaaataaa
     6421 attaaatttt ctaaaactaa aagagttttt gattcttcta aagtagactc ccatagtgct
     6481 ataattccaa cttatataat tccaaaaagt ttatctaagg atgaacaatt agtttatgat
     6541 gccataaaag atagatttgt agcaaacttt atgcctcctg ctgaatatga gaatacagaa
     6601 attaagactg aagtagataa ttgtactttc ttaacaaaag gtaaggtgct taaatcaaaa
     6661 ggataccttg aggtatataa taaggaagaa aaaaacgatt tacttccact tgtaaataaa
     6721 gatgatgttg tagatgtgtt agaaataaaa ccattaacta agcaaactac tccacctaaa
     6781 ccatatactg aggacacact tttaaaggct atgaaaaatt gtggtaaaaa tgtgcctgaa
     6841 gaagatacaa cagtgctatc tggatattcg attggaacat cagccacacg tgctgatgtg
     6901 ttaaagaaga taagtcaagt gggctatgta aagaaaaaag gtaaatctta ttctataaca
     6961 gaccttggga aaaatttagt tgaaattttt cctgttaaag acttatttga tgtagattat
     7021 actggaaaac ttgaaaaaag tttaagcgat attcaaaaag gaaagtatac aagaaaagaa
     7081 tatcttacta atatcattag ttttatatat caaaatgtca atattataaa acaagatgct
     7141 tcaaaaaata taaacacaga agcatatact ttcgattcaa aaactaaaaa atttactaaa
     7201 acaaaagaaa aaaaacagaa aaaagatgta actaatactg aaaaagaagt tgctatatca
     7261 aaggattcta ataacaaaaa agaagttaaa aatggtgatg aaaatcaatc tcttggcaaa
     7321 tgccccattt gtcaaggtga tgtactagaa tttgacaaag gctttgcttg taaaaatcat
     7381 aaagaatgta aatttgttat ttggaaaaat gataagtatt taagtcttta taaaaagaaa
     7441 gttaacaaaa caatggtcaa aaatatttta aaaaaaggtg aaactaaagt aaaatcttta
     7501 actgctaaaa acggtaataa atttgatgct acattaaaat atgataaaaa tccagatact
     7561 ggctatttca attggaaaat agagtttgac aactaaat
//
LOCUS       DAGSHT010000161         7582 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGSHT010000161 DAGSHT010000000
VERSION     DAGSHT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.167"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1357
                     /locus_tag="MJB72_002897"
     CDS             <1..1357
                     /locus_tag="MJB72_002897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263972.1"
                     /translation="IFMIEKLNANASLSDLITAFENSTNEIKTISNTLKQTLINKNIK
                     VLDTDKLSSLVDKVDKLKAQEFGVRINKLDENPDTCVSYLGDAIGMTPASVGSYGSWD
                     TIDFIRNIKPCGFKDGIVTKYIKKENFNMYEDGTNVEDDTDVMIEFPKFYWKIQSSDN
                     YMDIFISKTKLDDDYECPAHLIGSTEKEFIYIGAYLASLENNNLRSKKNSSPELRERG
                     RFYSLIYKHVPGYDSLDYQCVLMLQILFVVMFKSINFFRLGVGYSSYISGHGKPDKIN
                     TGGSNFKGMIYGEQNGDEQMKFLGIEDLWGNCLQEVGRCTCGVENACVYTRIITRDKR
                     NIQYGVGRKVGTISDVIGGNNTGFMVKQCLPGGGNGQDSRYYRCWANLSENHIGYYTG
                     FDYNENFRYYGLFSSYFQTEVKKASTRLIFIGEDNQNTLKTIQEDELLFVENEKVGGI
                     L"
     gene            1357..1536
                     /locus_tag="MJB72_002898"
     CDS             1357..1536
                     /locus_tag="MJB72_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263973.1"
                     /translation="MIDGVVVRILADRILNKGLNPLKNRVFELDDVTNTEYRIAVENY
                     IIEHSEVVEGAELTK"
     gene            1577..1807
                     /locus_tag="MJB72_002899"
     CDS             1577..1807
                     /locus_tag="MJB72_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBY8263974.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            1827..2084
                     /locus_tag="MJB72_002900"
     CDS             1827..2084
                     /locus_tag="MJB72_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBY8263975.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQFKDGEK"
     gene            2345..3208
                     /locus_tag="MJB72_002901"
     CDS             2345..3208
                     /locus_tag="MJB72_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBY8263976.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            3287..4099
                     /locus_tag="MJB72_002902"
     CDS             3287..4099
                     /locus_tag="MJB72_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8263977.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKRLYILNSSKPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            4341..5066
                     /locus_tag="MJB72_002903"
     CDS             4341..5066
                     /locus_tag="MJB72_002903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263978.1"
                     /translation="MKDIIEIYDNYNNIGVKNRTITEGKEIELVREYINFRKSEFQPT
                     ENRKLAIFLEAKVDNAYPDIVFVEYSPENYINWNEARAELNKDDLKILYHIYITGGID
                     STGLVKQLGVNWKEAMLAIEKLSDADLIIRNYKKWKIKDEKKLTTYTIEAVEAKLNNW
                     DEVLQQSIINKNFASESYALSIMKSKPKEETLKKFRKFGVGVYLKNENGFKRIKKARS
                     VTMPVSFNSIFFNEWIGRIINLG"
     gene            5079..5921
                     /locus_tag="MJB72_002904"
     CDS             5079..5921
                     /locus_tag="MJB72_002904"
                     /inference="COORDINATES: protein motif:HMM:NF012298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HBY8263979.1"
                     /translation="MNDKQIIENFNVREVIKRFPKSGQKQVFLVNHKVYGKVILKLVQ
                     SGDERVKREIEIVTENNFYNVPKVICVGNYDSSEECGIYILEQYIQGMSLREIIKEKS
                     ITLSEELVLAETMLKIIVQMEQQKIVHRDIKPENILRNTEGEWYLIDFGIARALDMSS
                     LTFTKVEIGPHTPGYGAPELFQYNKKDIDSRADIFSLGVVLFEAVTGYHPFIKGDEMN
                     LNEIWYNTVTVMPESVVIEGDYNMQYISLIQTYMQKHVTRRPNTAQKALEWFYNVKDN
                     LLKG"
     gene            5929..7134
                     /locus_tag="MJB72_002905"
     CDS             5929..7134
                     /locus_tag="MJB72_002905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263980.1"
                     /translation="MELFLQFAWGMKQMVLDLSKDWSGATTILSPRDISPNQLERWVK
                     EFKKANVKTLFDPQCYYPKCNHKNLVQYNYWDSSFSTKMQGSFSFEDELIKSILYYNN
                     IAETHEFIIPSNMLTHDEKWFSRWLKNSEQLVNSARKIVRDRKIILTLAIPDSVLLQK
                     EDEVEKIIEATEKLDVDGYYIIAHPPQDKYLVDVPMWLINLTQLCAGLKLQSRKVIMG
                     YGNHQLLCLTATGIDAMATGTYLNVRRFTNKFEEDDSIKRKSIWYYYPAALSEYKMGF
                     LDAAYNNGVLQQMRPAKEMDRGYVDLIFSDVLPSSTAFKEPMAFKHYLNCVKVQMKSL
                     LKQTYKETITANEIMLENAMRRIEYMEKSGVYAQGRSFKDMVDVNMSALQRLDKNRGF
                     QLQQEWNNF"
BASE COUNT         3084 a          725 c         1413 g         2360 t
ORIGIN      
        1 tatttttatg atagaaaaat taaacgcaaa tgcaagttta agtgacttaa taaccgcttt
       61 tgaaaatagt acaaatgaga ttaaaacaat ttcaaataca ttaaagcaaa ctctaataaa
      121 taaaaatata aaagtattag atacggacaa attaagtagt ttagtcgata aagtagataa
      181 gttaaaagcg caagaatttg gtgtaagaat aaataagttg gatgaaaatc cagatacttg
      241 tgtaagttat ctaggagatg caataggaat gactcctgct agtgttggta gttatggtag
      301 ttgggatact atagatttta ttagaaacat aaagccatgt ggatttaaag atggaatagt
      361 aacaaagtat attaaaaaag aaaattttaa tatgtatgaa gatggaacta atgtagaaga
      421 tgacacagat gttatgattg aatttcctaa attttattgg aaaatacaga gttctgataa
      481 ctacatggat atatttatat ctaaaacaaa attagatgat gattatgaat gtccagcaca
      541 tctaattggt agtacagaaa aagaatttat ttatataggt gcatatttag catcattgga
      601 aaataataac ttaagaagta aaaaaaatag tagcccagag ctgagagaac gtggacgatt
      661 ctacagttta atatataaac atgttcctgg atatgattct ttggattatc aatgtgtatt
      721 aatgttacag attctatttg ttgtaatgtt taaatcaata aactttttta ggttaggagt
      781 tggttatagc tcttatatat caggtcatgg aaagccagat aaaataaaca caggcggttc
      841 taatttcaaa ggtatgatat atggagaaca aaatggagat gagcaaatga aatttttagg
      901 aatagaggat ttatggggaa attgtctaca ggaggtgggg agatgcactt gtggagttga
      961 aaatgcttgt gtatatacaa gaataattac aagggataaa agaaatatac aatatggagt
     1021 aggaagaaag gttggtacaa tatcagatgt aataggtggt aacaacactg gttttatggt
     1081 taaacagtgt cttcctggag gaggaaacgg tcaagatagt cgatattata gatgctgggc
     1141 taatttaagt gaaaaccata taggatatta cacagggttt gattataatg aaaattttag
     1201 atattatggt ttattttcat cttattttca aacagaggta aaaaaagcat caactagatt
     1261 aatttttata ggagaagaca atcaaaatac actaaaaaca attcaagaag atgaattgct
     1321 gtttgtggaa aatgaaaaag ttggaggaat attgtaatga tagatggagt agttgtaaga
     1381 atactggcag atagaatatt aaataaggga ttaaatcctc taaaaaatag agtatttgaa
     1441 cttgatgatg ttactaacac agaatatagg atagcagttg aaaactacat tatagaacat
     1501 agtgaggtag tagaaggagc agaacttaca aaataggtaa tgttcttttt ttaatacaaa
     1561 gcaataggag gttttcatga atgaagaact tgtgaaagaa aaaataagta cacttgaaac
     1621 aagagtaaaa gaacacggca aacaaattga tagaatcgag attgagcaag caaagtttgc
     1681 tatacaaata caaaacctat gtagtgatat aaaaaatctg acaggagtac tcaagtggct
     1741 tgtaggagtt ataattacaa ctttagtagg gttctttata tttgccatac aaaaaggaat
     1801 attttaatta attaggaggt taaaagatgg ataatttaat aagttttata ccagagcagt
     1861 tactaatttt agtagctgct ctttatgtta taggagcagg ttgcaagaaa tataagcaat
     1921 tagataataa atatattcca gtagtgctat tgatacttgg aataggtttc tcaatatgga
     1981 tgctaggatt aaatcctgtt gcagtcttac aaggtgtaat ttgttgggga gttgcaatag
     2041 gtataaatca aacttacaaa cagtttaagg atggtgaaaa gtaatgaaat taacaaaaat
     2101 attattactc acaaaaattt aaaaaatttc attaaataga gtatactttt atagctaata
     2161 agtatataat aatagtacag aactcgtaaa gcatttatat gctcaaatat acgggacatt
     2221 gatttttgaa ggaacccgcc aagcttcttt tatagctcaa ataggcggga cagataaatt
     2281 tacccactaa tattaattgg tgggttttat tatataaaag tatatctatg aaaaggtgaa
     2341 aacaatggtt gaagtaaaag aagaaaaaac atttgatgaa caaatagata ttttaaaaag
     2401 tagaggatta ataataaatg ataaagaaga tgctaaattt gtattaagta atgtcaatta
     2461 ttataggttt acagcatatc ttctaagttt taagaatgat gatggctcat ataaagaagg
     2521 aactacgttt gaagaagttt atgatatata taggtttaat aaggaattta ggatattatt
     2581 aacagatttg ttagggagca tagaaatagc atttaggaca tacattgcat atacattagc
     2641 aattaaacat ggtgcttgtg gatatctaga aagggagagt ttcaaagatg aaaaattcta
     2701 tattaatttt ttgacagcat tagagagaga aaaaagtaat aattcagata agctttttat
     2761 tatacaccat aaagaaaaat atgaaggaaa acttcccatt tgggttgcaa cggaaataat
     2821 gacttttggt atgttgtcaa aattatattc aaacatgttg ccagaagata ctagatacat
     2881 aaaaaataat ttgtgtagag tgaatacttt attagttaaa tcttggttac aatcattaac
     2941 acaggtcaga aatcaatgcg ctcattatgg tagaatatat aataataatt tccgtattat
     3001 aacaataaaa aacgaatata aaaagtataa cttggataat aaaaagatat tttcttatat
     3061 acttgctatg aagcatttga ctatggataa attaatttgg aatagttttt ttataaaact
     3121 tcaaaagtta attaatgatt ataataattc tatagactta aagcttattg gttttcctaa
     3181 taattggata gagatattgg ctaaataaaa tagttacttt aagaagttta taaacactta
     3241 ctatatgtaa gtgttttttt attgaaaaga aggaggaaaa taaataatga aaatatgtat
     3301 tacagtagga cacagtattt taaaaagtgg tgcatgcact tctgctgatg gggtagttaa
     3361 cgaataccaa tacaataaat ctcttgcacc agtattagca gatacattta gaaaagaagg
     3421 tcataaggca gatgtaataa tatgccctga aaagcagttt aaaactaaag cagaagaaaa
     3481 gacttataaa atacctagag ttaatagtgg aggatatgat ttacttatag aactacatct
     3541 aaatgcaagt gatggtcaag gaaaaggttc agaagttcta tattatagta ataaaggctt
     3601 agaatatgca actagaatat gtaataagct aggtacagta tttagaaata gaagagctaa
     3661 attagataaa agattatata tcttaaatag ttcaaagcct acagcagtat taattgaaag
     3721 tttcttctgt gataataaag aagattatga gaaagctaag aaactaggtc atgaaggtat
     3781 tgctaagtta attgtagaag gtgtattaaa taaaaatata aataatgagg gagttaaaca
     3841 gatgtacaaa catacaattg tttatgatgg agaagttgac aaaatccctg caactgtagt
     3901 tggttggggt tataatgatg ggaaaatact gatatgtgat ataaaagatt acgtgccagg
     3961 tcagacgcaa aatctttatg tcattggtgg tgcagcatgt gagaagattg gttctatgac
     4021 taaagaaaaa tttactatga taaagggtaa tgatagattt gatacacttt ataaagcatt
     4081 agattttatt aatagataaa ttaaaaagta gcaactaaaa gtagttgtta tctttctgaa
     4141 aattaagagt tgtatcaata agttgagtct gattagtagg aatttaagag gtacattttg
     4201 ataaaataac gaaaaagcag aagtctcatg aaaagaagaa tattatctaa aaagaatggc
     4261 attataatca ataaagatga tataattaaa aaagtaaagg tacgaagata ctgaaaatta
     4321 ttgtttaaag tggtggtaat atgaaagata taatagagat atatgataat tacaataaca
     4381 taggcgtgaa aaatagaacg attacagaag gtaaagaaat tgagttagta agagaatata
     4441 ttaattttag aaaaagtgag tttcaaccaa ctgaaaatag aaagttagca atttttcttg
     4501 aagctaaggt tgacaacgca tatccagata tagtttttgt tgaatattca ccagaaaatt
     4561 atataaactg gaatgaagca agagcagaat tgaataaaga tgatttgaaa attctatatc
     4621 atatttatat tacaggagga attgattcta caggattagt aaaacaatta ggagtaaatt
     4681 ggaaagaggc aatgttagca attgaaaaat taagtgatgc agacctaatc ataagaaatt
     4741 ataaaaaatg gaaaataaaa gatgaaaaaa aattaacaac ttatacgata gaagctgtag
     4801 aggcaaagtt gaacaattgg gatgaagtat tgcaacagag tattataaat aagaattttg
     4861 catctgagag ttatgcatta tctattatga aatcaaaacc aaaagaagag acactaaaaa
     4921 aattcagaaa gtttggtgtg ggggtatatt tgaaaaatga aaatgggttt aaaaggatta
     4981 aaaaagctag aagtgttact atgcctgtaa gttttaattc tatatttttt aatgaatgga
     5041 taggtaggat tataaactta ggttaagggg gtaaagtgtt gaacgataaa cagattatag
     5101 aaaactttaa tgttagagaa gtaataaaaa gatttcctaa aagtggtcaa aaacaagtct
     5161 tcttagttaa tcataaagta tatggaaagg ttattttgaa gttggtgcaa agtggggatg
     5221 agcgcgttaa aagagagatt gaaattgtaa cagaaaataa tttttataat gttccaaagg
     5281 taatttgtgt tggtaattat gatagttcag aagaatgtgg gatttatatt ttagagcaat
     5341 atatccaagg aatgtcatta agggaaatta tcaaagaaaa aagtataaca ttaagtgagg
     5401 agcttgtgtt ggcagaaact atgttgaaga ttatagtgca aatggaacag caaaaaatag
     5461 tgcatagaga cattaaaccc gaaaatatat taaggaacac agagggagaa tggtatttaa
     5521 ttgattttgg aattgccaga gcgttagata tgagctcatt aacatttaca aaagttgaaa
     5581 taggacctca tacacctgga tatggagcac cagaactttt ccaatataat aaaaaagaca
     5641 ttgatagtag ggctgatata ttttctcttg gtgttgtatt gtttgaggca gttactggtt
     5701 atcatccttt tataaaagga gatgagatga atttaaatga aatttggtat aatacagtta
     5761 cagtaatgcc agaaagtgtt gttattgaag gtgattataa tatgcaatat ataagtttaa
     5821 tacaaaccta tatgcaaaaa catgttacca gaaggccaaa tacagctcaa aaggctctag
     5881 aatggtttta taatgttaaa gataatttat tgaaagggtg agaatagaat ggaattgttt
     5941 ttacagtttg catggggtat gaaacaaatg gtattagatt tatcaaaaga ttggagtgga
     6001 gctacaacta ttttaagtcc tcgagatata tcacccaatc aattagaaag atgggttaaa
     6061 gaatttaaaa aagcaaatgt taaaacatta tttgacccac agtgttatta tccaaaatgt
     6121 aatcataaaa atttagttca atataactat tgggattctt cattttctac aaaaatgcaa
     6181 ggaagttttt catttgaaga tgaactaata aaatcaattt tatattataa taacattgca
     6241 gaaacacatg agtttattat tccatcaaat atgcttactc atgatgaaaa atggttcagt
     6301 agatggctaa agaattctga acaattggta aactcagcaa gaaaaatagt aagagataga
     6361 aaaataatac taacattagc aattccagat agcgtattgt tgcaaaaaga ggatgaagta
     6421 gaaaaaataa ttgaagcaac agaaaagttg gatgtggatg gatattacat aattgcacat
     6481 ccacctcaag ataagtactt agtagatgtt ccaatgtggt tgattaactt gacacaatta
     6541 tgtgcaggat taaaattaca aagtagaaaa gtaataatgg gatatggaaa tcatcaatta
     6601 ctttgtttga ctgcaactgg aatagatgcg atggcaacag gtacatattt aaatgttcgt
     6661 agatttacta acaaatttga agaagatgat agtataaaga gaaaaagtat ctggtattat
     6721 tatcctgctg cattatctga atataaaatg ggttttttag atgctgcata taacaatgga
     6781 gtattgcaac aaatgagacc agctaaagaa atggataggg gatatgttga cttgatattt
     6841 tctgatgtgc ttccatcttc aactgcattt aaagagccaa tggcctttaa acattatcta
     6901 aattgtgtta aagtacaaat gaagtcgttg ttgaagcaaa catacaaaga aacaattaca
     6961 gcaaatgaaa taatgttgga aaatgctatg aggcgtattg agtatatgga aaaaagtggg
     7021 gtatatgcac aggggagaag ttttaaagat atggttgatg ttaacatgtc agctttacaa
     7081 cgccttgata aaaatagagg attccaatta caacaggagt ggaataattt ctaatgattt
     7141 atcatatcta ttttcttatt taaacaaagt aaatgaaatt tggtttatat aaggaaaaga
     7201 agtgatattt aaaagagaat ataaacttag tgaaaacaat taatattact gatagagatt
     7261 tcaactgatt ttcaatgttg ttttgataat agaattaaga atttactaat ttcatttatt
     7321 aaaaaaattg aaaagaactt aaaatgggaa atactctaga taagaacggt ttttataaat
     7381 tatcaaaaaa cattttaaat tgtctttaaa atcaatttaa aaaagttttt tcatagtatt
     7441 aattatatta tataataaaa aagtaagact gtattagtat tacattttta ctgaattaat
     7501 tattattatt aaagagagca ttttccatta gtgggagtgc ttttttcatt tatttaaaaa
     7561 acactatttc ttttatcaat aa
//
LOCUS       DAGSHT010000162         7578 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.168, whole genome shotgun sequence.
ACCESSION   DAGSHT010000162 DAGSHT010000000
VERSION     DAGSHT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.168"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..361
                     /locus_tag="MJB72_002906"
     CDS             <1..361
                     /locus_tag="MJB72_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY8263981.1"
                     /translation="EEKAKIFLEMGILTYQKIREDSINDNSFDKLCDELLELDKLIYE
                     KNMEINEMEMAESNVTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDT
                     EAEFCPCCGRKIIIDLE"
     gene            634..1305
                     /locus_tag="MJB72_002907"
     CDS             634..1305
                     /locus_tag="MJB72_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBY8263982.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            1390..2256
                     /locus_tag="MJB72_002908"
     CDS             1390..2256
                     /locus_tag="MJB72_002908"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBY8263983.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            2289..3791
                     /locus_tag="MJB72_002909"
     CDS             2289..3791
                     /locus_tag="MJB72_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBY8263984.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            3921..4373
                     /locus_tag="MJB72_002910"
     CDS             3921..4373
                     /locus_tag="MJB72_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBY8263985.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            4533..5336
                     /locus_tag="MJB72_002911"
     CDS             4533..5336
                     /locus_tag="MJB72_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8263986.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            5394..6281
                     /locus_tag="MJB72_002912"
     CDS             5394..6281
                     /locus_tag="MJB72_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY8263987.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            6300..7148
                     /locus_tag="MJB72_002913"
     CDS             6300..7148
                     /locus_tag="MJB72_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8263988.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            7211..>7578
                     /locus_tag="MJB72_002914"
     CDS             7211..>7578
                     /locus_tag="MJB72_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBY8263989.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQL"
BASE COUNT         3061 a          721 c         1392 g         2404 t
ORIGIN      
        1 agaagagaaa gcaaagatat tcttggaaat gggaatactg acatatcaaa aaattagaga
       61 agattctatt aatgataata gttttgataa attatgtgat gagttactag aacttgataa
      121 gttaatttat gaaaaaaata tggaaattaa tgaaatggaa atggcagaaa gtaatgtaac
      181 ttgtgagtgt ggttatgttg gaaatgtaaa tgataaatat tgtgctgaat gtggtaaaaa
      241 atttgaattg gaaaatgaat gtgatgattt tataatttgt ggttattgtg aaagtgagat
      301 agatacagag gctgaatttt gtccatgctg tggaagaaaa attataatag atttagaata
      361 atattgggaa acctatgtat agtatacatg ggttttttta ttgaaattat ttaaatggac
      421 ttataacagt agttatataa aatgaaaata aacaccgaaa aaatgaaata aaatttcttt
      481 taaatgtaaa aaacaagcta atatataaaa taaatcagta aaaatataga tgtgagaaaa
      541 aaatatgaaa aaatattgca aagaaaaagt tttcatgtta ttattaatac ataaatcaag
      601 aaaataaatt tcagaaacga ggtaatttaa aaaatgctag ataaggtaaa gggaagatta
      661 atagtatcat gccaagcttt agaagatgaa cctcttcata gtccttttat aatggggaga
      721 atggctaagg ctgctatgga aggtggagct gttggaatta gagctcaagg agttgaagat
      781 attattgaaa taaaaaaagt tactggactt cctgttatag gtattataaa gagaaactat
      841 gaagattcag atatatatat aacgccaact aaaaaagaag ttgatgaact tttaacaaca
      901 gggtgtgaaa tgatagcatt agatgctacc aatagagtta gaccaaataa tgaagactta
      961 aaagaactta taaagtatat aaaagaaaac ggtgttctag taatggcaga tatttcaaat
     1021 tatgatgagg ctatcaaagc acaagagtac ggtgtagatt gcgtttcaac tacactatct
     1081 ggatatactc catatactaa aacattagaa ggtccagatt ttgtattaat ggaaagatta
     1141 gttaaagatt tagaaatacc tgtaatagct gaaggaaagg taaatactcc tcaagattta
     1201 aaaaaggtct ttgagttggg tgttcattca tcagtagtag gttcagcaat aacaagacca
     1261 caattaataa cagaaaaatt tgttaaagca attgaaatta attaaaaatt aatttataaa
     1321 ttaaaaaatt attatattta actatatttg aaaattaaaa ttggaggtaa agatatgaga
     1381 acaacagata tgaagggaat ttattcagca ttattagttt catttgataa ggaaggaaat
     1441 ataaatgaaa aagggttaag acaaataatt agacacaata tagatgtttg taaagtagat
     1501 ggtctttatg taggaggaag tacaggggaa aacttcatgc tttcaactga tgaaaagaaa
     1561 agaatatttg aaatagctaa agatgaagtg aaagaagaaa taaaattaat agctcaagtt
     1621 ggttcagtta atttaaaaga agctgtagaa cttgctaaat ttacaactga tttaggatat
     1681 gatgcaataa gtgctgttac acctttttac tataaatttg attttgaaga aataaaacac
     1741 tattataata caataataaa ttctgtagat aatagattaa taatatactc tataccattt
     1801 ttaactggtg ttgatatgag tttagaccag tttggagaat tatttgaaaa tgaaaaaata
     1861 ataggtgtta agtttacagc agcagatttc tacttattag agagaatgag aaaaacattc
     1921 ccaaataaat taatatttgc aggatttgat gaaatgatgt taccagctac agttttaggt
     1981 gtagatgggg caattggttc tacattcaat gtaaacggtg tcagagctag acaaatattc
     2041 gaattaacta agaatgaaaa aatatcagaa gcacttgaag tacaacatgt gactaatgat
     2101 ttaataacag atatattagg aaatggctta tatcaaacaa ttaaattact acttgaagaa
     2161 caaggtgttg aggctggata ctgtagacaa cctatgaaag aagcaactga tgaaatgaaa
     2221 tcaagagcaa aagaaatata cagaaagtat ttttaataaa atacttttag tatgggagga
     2281 ttagtcgaat ggtaggattt acttggatag attttgcaat actagtggtt tacttattgg
     2341 cagttttatt tgcaggattg ttattttcaa aaaaggaaat gaagggaaaa gagttcttca
     2401 aaggtgatgg aactattccg tggtgggtta catcagtatc aatatttgcc acattattaa
     2461 gtccaatatc atttttatca ttagcaggaa attcttatgg tggtacttgg attttgtggt
     2521 ttgcacaatt aggtatgttt atagcaatac ctttgacaat aaaatttttc ttacctctat
     2581 atagtaggtt agaaatagat acagcatatg aatacttaga aatgagatac gaaagtaaag
     2641 gtcttagagt acttggagct ttgatgttta taatatatca aataggacgt atgtctatta
     2701 taatgtatct tccatcaata gttctggctg gtcttacagg aatacctgta aatgtactga
     2761 taatagttat gggtgtaata gcaataattt actcatatac aggtggatta aaggctgtct
     2821 tgtggaccga ttttatacaa ggaatggtat taatagtagg tgttacaggg acattgttct
     2881 atttaatagc aagtataaat ggtggttttg gaacagtaat ggatactctt acaactggac
     2941 acaagttctt agcaagtaat gaagttatgt ttgataaaaa tatactatct acaagtgcat
     3001 ttataatatt tgttggagct ggtcttaata ctttttcatc atatgtatcg agtcaagata
     3061 tagttcaaag gtttactaca acaactgata taaagcaact taataaaatg acatatggaa
     3121 atggtgtttt atcaatggga cttgcaacag tattttatct aattggaact tgtttattta
     3181 tatactatac tcaaaaccca gatttagctc aaacagttca acaagaccaa gtatttgcat
     3241 catatatagc ttatgaatta ccagtgggta taacaggaat actacttgca gcgatatatg
     3301 cagcttctca atcaacttta tcgacagggc ttaattcagt tgctacaagt tggactttag
     3361 atatacaaac tatcataaca aaagatataa gtatggaaaa gcaaactaag atagcacaat
     3421 atatatcatt aggtgtaggt atactttcta tagctgttgc aatagtatta gcaaatggag
     3481 aaataaagtc agcatatgaa tggtttaata gttttatggg gctagtacta ggtgttttag
     3541 caggaatatt tgtacttggt gcattttgta aaaaagcaac taaaatggga gcatatgttg
     3601 gattcatagt atcagcaatt ttagttgttt atttaaaata ccgtatgcca gaagtaactt
     3661 cttgggcata ttctctaata acaatcacga cttctgtagt ggtaggtaca atagttagtg
     3721 caatagaagc taaagttaaa gcaaaagtat ctttaccaaa agctgagact actgtttatt
     3781 atgaaaaata atatttaaat gagaaattgt cttatagagt gattagataa ataatttagt
     3841 tactctataa gattgtataa tatactttgt atacaaaagt ataaaaatag actttataat
     3901 tatataggga gatagtgctt atgatatttg gaaatataaa tcatagtaag acatattcaa
     3961 cattgcatga agatttactt agatgttttg aatatgctaa agataattca ttaatagatt
     4021 atgaaaaagg tacttataca atagatggtg atgatatttt tgtgaatatt gtagagtata
     4081 aaacttgtga aaaagaagat agattctggg aagctcataa gaagtatata gatgttcatt
     4141 tgatgcttga tggatgtgaa cgaatagata taaattttat agaaaattta gaacaaaaaa
     4201 tatatgaaga aaagggcgat ttcttgcctc ttgatggtaa aaataatgga tacatggaac
     4261 ttagaaaagg tgatttttta gtttgttatc cagaagatgc tcatatgaca ggaataaaag
     4321 ttttagaaaa agaaaatatc aaaaaagcta tttttaaggt tttggttaga taatgataat
     4381 ttatataacc actagggctg aaaatagtga tttaactttc agcgattagt gagacttcgt
     4441 aaattttagt tagattacta ttttcagcct tagtttttta tgagaaaatt gaaaataatt
     4501 aaaagcttaa aattttgtaa aggagttaac ttatggaaaa tatagatgta ttcttattaa
     4561 taggacaaag caatgctaga ggattgggaa atccaaaaga gagcatcatt ccaaatgaaa
     4621 attgttttga atatttgagt actgatgaaa ttataaatat gagatgtgag ctagagactt
     4681 cagaaggtga tggtactata gcacctgcat tttcaaatga atggaataag cttactggaa
     4741 ataaagtttg ttttattcac aatgcaaaag atggttcaag aataaaaaac tggaatcatg
     4801 ataataactg gtttctaaat gacactatag aaaaatttaa tacaggctgt actaccttaa
     4861 gtaaacatta taatatagaa aataaatacg ttatttggat tcaaggtgaa agtgatgcaa
     4921 aatatggtag tgatgtattg tactataaag aaagtctaaa aaaaattgcg tataggctta
     4981 aagaagaatg tatgattgat aaaatgtttg taagtttaac tggttattgg cttggagaag
     5041 atgaatattt tattagaaca agacgaattg cagcagcaca ggagtcggct tgtagtgaat
     5101 gtgatatatt atgtgtagga agcaaaattg ctatgcagtt tcatgacaga ggattaacta
     5161 ttgatgatgt tcattacact caagaaggtc ttaatatgtt aggagaggac ttatgtaaaa
     5221 atatttataa gtatcatata accaaagaaa aaagtgtatt aagtgatact atagacttat
     5281 cagaagcaag gaaatatatc tgtgaattgg aaaagataag taaaagattt gtgtaatata
     5341 aaattttaaa tagagttgtt aatttataaa aataatttgt atggagatat gttatgaaaa
     5401 attacgtttg tatagatgtt ggaggtactt caataaaata tggttttatt agagaagatg
     5461 gatttataat tgataaatca agtcttgata ctgaagcaaa ggaaaaaggt ggagaaggaa
     5521 tattagccaa aataaaagat atagtaaaaa aatatataga ggaaaatgaa ataagtggta
     5581 tatgtatatc aactgcaggt atggttgacc cagttgaagg taaaatacta tttgcattag
     5641 aagaattaat tccaaactat aagggcatgc aattaaaaaa agaagtagaa aaagagttta
     5701 atataaaatg tgaagttgaa aatgatgtta attgtgctgg acttggtgaa atgtggcttg
     5761 gagcaggtag aggtgcaaca tcttctatat gtttaacaat aggtactgga attggtggat
     5821 gtattataat aaataataaa ctgataaatg ggtttagtaa tagtgctggt gaagtaggat
     5881 atatgaatgt aaatggaagt tcttttcaag aattggcatc tactagcagt ctaattaaaa
     5941 aagtagcaaa aataaaaaat ttgaatgaaa atgacttaaa tggaaaaata atttttgatt
     6001 tagcaaagaa taatgatgaa gattgtttaa aagaactcga taatatgata aaatcattag
     6061 cagttggaat cgcaaatata tgttatgtaa taaatccaga agtggtaatt ttaggtggtg
     6121 ggattatggc acaagaaaag ttcctaaaac caaaaataga caaagcatta aaggaggttc
     6181 ttatagagag agtttacaaa aacactaata tagaatttgc aaagagacaa aatgatgcag
     6241 gaatgcttgg agcattatat aattttttaa acaaaatata aagaatagaa gggtttttaa
     6301 tgagtatttt agaacaattg gaatcaccaa cttttaaagt cacaaaatca gataaattat
     6361 taatagctta tataaaagaa aatatagatg atgtatttta taaaccaata tcacaaatag
     6421 ctaaagagag caatattggt gaagcaacta taactaggtt tgtaaaaaaa atgaatttta
     6481 atggattaca ggattttaaa gttactttag cacaagaaat atcaactatc aataaaaaaa
     6541 atattataaa taaaaatata caaaatgatg agccagcttt ggatacagca aaaaaacttt
     6601 taagttcaaa tgtaaccact ttagagaaca cagtagagat aatcaatagt aaggatgtac
     6661 atgactgcgc aaggcttata ataaatgcaa agaaagttta cttcataggt attggatatt
     6721 ctggtataat agcacaggat tcaaactata aatttatgag aataggtctt aattgtgtaa
     6781 gttttgacag tagtcataca atgataatga tgtcttccat aatggaagaa ggagatttaa
     6841 taattgcaat ttcacattct ggtgaaacag aagagattat aaaaactgta aagcttgcaa
     6901 gagccaataa tgctaaaatt atatcaatta ctgaaaataa aaattctgaa ttaaaggata
     6961 tttcagatgt acacttatct tatgtatcgg gagaaacagt actggaaacc ggttcaatat
     7021 cttctaaatt agcacaattt ttcattattg atttagtata tacacaggta gtaaaagaac
     7081 tttctaatga agctattgaa aggaaaatta aaactacaaa tgctataaaa ttgtttaaaa
     7141 atgaataaaa taagaaattt gtaatgtttt atagagctct cttaaaaatt taggaatgga
     7201 gagatattac atgtctaaat taaaagatta taatattgat aaagtaataa tttttaataa
     7261 tttaagtact aaaacgaaga atcaacttaa agaaaaagtt aaactaaaaa gattacaaaa
     7321 aaaagaaatt ttattttatg aaaaagatag cttagacagt gtttatatac ttttagaagg
     7381 aaaggtatct atttttaaga taagtgaaaa tggagagaga aaagtaattt tcatattaaa
     7441 tagtggggaa gtcataaatg atattacact tgacgcttct aaaaactcta ctgttggctg
     7501 tgaagcattt gaaaattcaa ttgtagctta ttatagtatt gaagaatttt tagaaattat
     7561 gcaaaatgat tttcaact
//
LOCUS       DAGSHT010000163         7507 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.169, whole genome shotgun sequence.
ACCESSION   DAGSHT010000163 DAGSHT010000000
VERSION     DAGSHT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.169"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..212)
                     /locus_tag="MJB72_002915"
     CDS             complement(<1..212)
                     /locus_tag="MJB72_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8263990.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVV"
     gene            complement(468..2702)
                     /locus_tag="MJB72_002916"
     CDS             complement(468..2702)
                     /locus_tag="MJB72_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8263991.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            3021..6449
                     /gene="uvrA"
                     /locus_tag="MJB72_002917"
     CDS             3021..6449
                     /gene="uvrA"
                     /locus_tag="MJB72_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY8263992.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLKKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCQQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            6673..7467
                     /locus_tag="MJB72_002918"
     CDS             6673..7467
                     /locus_tag="MJB72_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8263993.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YNNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
BASE COUNT         2803 a         1075 c         1023 g         2606 t
ORIGIN      
        1 actacttgga agtcatcagg aataattaac acacctttgt tcatcttttg tattactgac
       61 ttgtttaatg aacgcaaaac aatatttaaa ttatttatat catatctaac ttttctttct
      121 tctatactaa gttgtttgct aatatccttg taacttgtct tattttttcc ttttagaaaa
      181 ttcatgatga gtaacattct cccacttatc atattttccc tccttccaaa ataattagag
      241 ttattaatat agcaaatttt atattattat gtatgtttca gtttttatct aagacagtaa
      301 ctatctttct atattcagaa taatacaata aagctttagc cacaacttac aacatttgaa
      361 aattattttt acaaaatctg caaatatatt tttattaaat atataaataa aaaaggcaag
      421 ttatattttt aattatataa cttgcctaaa ttttttatct tgtacattta ataatggata
      481 tttttaattt tattattttc atatgcaaaa ttttcaaagt cagaaattaa taatggtttt
      541 gaaaaaacat aaccttgaat ataatcacat cccacatttt ttaggaactc aacttgttct
      601 aattcttcaa ttccttcagc agttactttc atacctagcc ttttgctaag ttcaataata
      661 gacttaataa ctaccttacc acggtctata ttatttttgc taacaaagaa tgaacggtct
      721 aacttaatta tatcaacttt aaaatccttt aatatcccta atgatgagta cccaaatcca
      781 aaatcgtcta tagaacattt aaatccatta gaatgaatat cctctattat atctatagct
      841 tctggatttt ctaagaaaat agattcagta agttctaaat ctatcagaca tgttggtaca
      901 ttgtatttat tgcatatatg cttatatttt tctaaaaaca atttatcatt cagatgttga
      961 cgtgatacat taacagaaat tctcaacact tctttacctt cttttatcca cttagaaatc
     1021 aatttgcatg tttcttctag tatatataaa tctaacttac atataaatcc acttttttcg
     1081 aaaagaggaa taaaatcact tggataaatt atgccctttt ctgaattttt ccaacgcaca
     1141 agagactctg ctccactaat ttttccactt tttatatcaa ttttaggttg taagcacata
     1201 aaaaattctt tattttcaag ggctttttcc attaaatttg aaatttcctt ttcagatatt
     1261 aatcgttttc tttctatatc actataaaaa gaaaaatcac tttgatgagt tttattaaag
     1321 ctttttcttg ctgtatttgc atggtcttgt attgtaatta aatcttcatt atgattttca
     1381 attgtgtaaa tacctactga tagtcttaaa aagtatggat tttccctatt tttattaaaa
     1441 taatttgtgt cagcttctat tttatataat aactccaggg cttctaacac tggtctattc
     1501 ttaataagca aatagaaaag gtctgcatca tgacggcaca caaactcttc attttcatca
     1561 atgtttttat acaaaatatt gtaaagatgt tttagtgtca tatttccctt ttcactaccg
     1621 aaacaatcat ttattagctt aaaatctttt atattcattg ctatcatcat ataagtacta
     1681 tcaggtgatt ctgaaattaa aggtttgact agcatactaa acttagttga atttatacca
     1741 tttgttatac tatctaaata tgcaatattt tctatttgat ttacatattt tcttcttata
     1801 atatataaaa ttaatgttat aattccaata atacatgtaa ttataaatgt cattgttatt
     1861 gtaaaagagt ttaatttatt aagttcacca agtacagcat cttcaggtac tatgagaaga
     1921 aaatgccaat catttaaacc tcttgtaatt ggatgataag ttaataagca ttgtacacct
     1981 tttgatgagg ttattgtaat attccctgat ttatcatttt taaaatcctc aagcatcttt
     2041 tgtgcccaag gttcatctcc agaactctta tatttatggc taaggtcctt tattaattca
     2101 gtattttttc ctatgaaaag aggttttccc ttactattaa taatttcaat agttcctttt
     2161 ccattaaagt tatctatatt aagaatctca ttcatacttt ctttattgga tacacctatt
     2221 aaaatactag atacttcatg attatcatta aatataggta cactataaag aacaaaaccc
     2281 attgactcta ttacttctga gtattcttta ccttcaattc cttttttata ggaaacagta
     2341 ttctttaagt caaacttttt tccacttaaa aactcagctt taccttctaa atctattatt
     2401 ccaacatcat aatacttata actagtcttt cttttagata aatactcata tcgactatca
     2461 ccttcatatt gcttaataaa atctgccatt attcttaagt cggataaaat aaatttgatt
     2521 ttagaatcaa cattcattac aatctgtgta gatacatcat ttaaatagtt tttagattgt
     2581 ttattagata tattgtgtag ttttacggta ttaaaaacag taattacaag gattgaaata
     2641 ataagtgaac ataaaattat agacagaact aaactatttt tcttatacct tgataatttc
     2701 atttttttcc tccaaattat aaacctattt tttaatttat attaacactt acaaattttt
     2761 ttactaaaaa tccttcacac tatattatag tattctattt cttaatattc aagaatgcca
     2821 tcttaacatt tcattaacat tcaatagata atggttttat taaataatta tattatatgc
     2881 aatttgaaac tatatttgta ttacaattta tcactgtatc attttttatt tatttttaaa
     2941 ttttggccaa aaaatctaaa ttgtccttgc atatgacacc atgtcagggt atatcatcca
     3001 tttagagagg tgaaatataa atgaaaataa ataactataa aaatcaaagt atcatcacaa
     3061 atcctaaaaa atttgaaaat aaatatcaag atttaccaaa gactccaatt gagttattaa
     3121 aagtggttca aagcctagta attcatggag accaaggaaa attatatggt atatccttta
     3181 ataaacaaca atctgatgag gaattgcttc gtacaattcc tcaaatgtta aaaagaatat
     3241 ttgaaataaa ttctaaccca ctaactattc caagaaaccc aaaacaaagg cttgttggaa
     3301 tgtgcagaga ttattcttta cttcttgtat cgctcctaag atatcgaggt tttgaggcta
     3361 gaatgagagc aggttttgct aattattttg aaagtgagct tacttatgaa gaccactggc
     3421 ttgtagaata ctatgatact ctaaaaaaga gatggataag aatagatgca caaatagatg
     3481 acatacaaaa aaattatttt caaattaatt ttgacactca tgatgtcgga aaaacagatg
     3541 gttttttaac tggttcagaa gcctggataa gatgtcagca aggacatgct catccagatg
     3601 actttggata taataaaaat tggaaaggtt ggcattctgt aaagggtaat cttctacatg
     3661 actttaataa tatgattggt ttagaattgc ttccatggga tttatggaca gaattaagtt
     3721 ccaaaaaata caatcaatta acaagagcag aaaaaaattt attagatgaa atggcagaga
     3781 tattatcaag cggaaatata aaaatagaag accttaattt gttaatagaa aaattgcctg
     3841 aagattattt gaaatcaatc ttttctcagt taaaaatatt aggtatttcg gaaataaaag
     3901 aattaggcaa tcctcttgaa ctagaaaaga aattcaaatt tacaaaatca ataaataaat
     3961 caattaaaaa ttcactttgt cataataaaa gctctatcta tttaaaagga ggaaggcaaa
     4021 acaatttaaa ggatgttgag gttacaattc caaaaaatca aataactgtt attacaggtg
     4081 taagtggtag tggaaaatct tctttagctt tcgatacgat ttatgaagaa ggaaaaagac
     4141 gttattttga aaatctaagt aatggagcaa aactttcaga acagcttcaa aaaccggaat
     4201 ttgatttact tcagggatta acccctacta tagctatcga acagaaaaaa ggaagccaaa
     4261 atcctcgctc tactgttggt actcttacaa gcatttggga ttatttaaga atgttatttg
     4321 tatcaatcgg aaaaagttac tgtccatatt gtaaaattcc attagaaaaa aagaataata
     4381 caaaaaatta ttgtccgcat tgtcaaacta ttttttctaa gataaatact tcaaccttca
     4441 atgctaactc ccatactgga gcttgtcacg attgtaatgg attaggtttt acctatcaag
     4501 ttaatcctca attgattgta aaagacccaa ctatttcaat attagatgga gccacttact
     4561 attttggaaa attgaggggg aaaaagccaa atggtaattg gatggtaggt gaattatatg
     4621 caatagcaaa agataaaaat atagacctag atataccttg gaatgaatta ccaagagatt
     4681 ttatagatgc aatcttatat ggtacagacg ataagatata tgaatttagc tttgaatcaa
     4741 agggacgaga atcaaagatt agaagacctg cttcaggagc aatcaaccac attcaaagac
     4801 tttttagaga atctagttct gaaaataata ccttgcatca atatatgaac aaaattcctt
     4861 gcaatacatg tggtggcgaa ttgctgtgca ttgaggctag attcactaca atcaaaggtt
     4921 atcgcttccc tgaactaaca aaaatgacga tagaacagtt atggaattgg ttgtgtgaat
     4981 taccaaatca acttcaaaaa aatgagctct ctttggtaaa tgatatctta acagaattaa
     5041 aaattcgagt tagttatcta ttaaaggtag gtcttagcta tatatcaaca gatagaacag
     5101 ctccaacttt atctggagga gaactgcaga gagtacgatt atcaagtcag ctaggaagtg
     5161 aattagtagg cttaacctac attttagatg aaccatctat tggtttacac ccaagagacc
     5221 ataatttaat catcaaaatg atagaagaac taagagataa gggtaatact gttatagttg
     5281 tggaacacga taaagataca atactgtctg ctgattatat tattgatgta ggcccaagtg
     5341 ctggtacaaa aggggggttc attattgcag aaggtacaac ccaagaaatt ataaaaaatc
     5401 caaactctat aacaggtaaa tatttaagta catacaataa aacaggttca caaaataaaa
     5461 ctataccaag taaatggtta agcttaaaag gatgccatgc aaataactta aaaaatattg
     5521 atgtagaaat tcccttaaac tgtatgtgct ctattacagg tgttagtggt tctggtaaga
     5581 gctctcttgt atttcacagt ttacttcctg ctctagaaga aaaattgaaa caaaaatcta
     5641 tcccagataa aaattatact gaatttactg gatttgatgc tattgatgat tttatcttaa
     5701 tggaccaaac acctattgga aaatctagcc gttctacacc tgctacatat atcaatattt
     5761 ttgatgaaat tcgttctcta tttgctgaaa ctccacaagc aaaacaaaaa ttattggatg
     5821 aaagctattt tagctttaat agcaaaaagg ggcaatgccc aaactgtcaa ggattaggta
     5881 aaacaaaaat tattctgcaa tatatggcag accaatgggt aacttgtagt gaatgtcaag
     5941 gtaagagata tcaaaaagaa atactgtcta tacaatacaa gggaaagaca attgcagata
     6001 tacttgacat ggaagtagca gaagcaaaaa cattttttag cgattgctct gatatatata
     6061 gaaaactctc cttattagat gaagtgggac ttggatatct aaagcttgga caaaatacat
     6121 taggattaag tggaggagaa tctcaaagga taaaattagc aaaagaatta gggacaaaaa
     6181 ctaaaaaacg aatgctgtac atattggatg agcctactac tggacttcat tttaaagata
     6241 tagaaaattt gttaattact tttagaaaac ttgtaaatga acagcattca cttttaatta
     6301 ttgaacacaa tacagaggtt atacgtgctt ctgattggat tatcgatata ggtccagaca
     6361 gtggaataaa tggaggggaa attgttgctt ctggaactcc tgatgaaata aaaataaatc
     6421 caaatagtat tactggacat tttatataaa aacaattaaa tacacatgaa cttaaatata
     6481 aaattataga atgtcttata ttatagaaga attattataa taggtgtaca ataaaaaaat
     6541 taactttagt atattatctg tgataaaata tgaataatat ataaaacaga acataaaaat
     6601 tattatattt tataatagag aaaggaaaat caaatgagtt ggaaacatat aaaaatacca
     6661 aatggagata caatgatttt ttgtaactca tttattacca gaaaatcatc catgaatatt
     6721 gttgttacac atactcctat tgtcactacg cttgagatgc aaaaagcata tgaaccactt
     6781 gccaaacata atgtaaatat atttgctttt gatttttcag gaacagggaa aagtgatgga
     6841 tatgaaaaag atttttctcg caattcaatt gtaaaagact tagattctgt cattacatac
     6901 atagaaaaaa attattcttc caacatacat ctatatggga atacgggaat tggtggtatg
     6961 tttgcacaat attatgcatg ttcagacaat aagttaaaaa gttttgctca gtttgcttgt
     7021 gttgattata aaaacacaac aggagtaggc tatacctatc tcactgttaa attattaaac
     7081 tttttcttta agtttcttcc taattttcac ataacagtaa aaccacctaa atataaaggt
     7141 tataatgaag tctataataa tgaattttat aaaatatttg aaaagaaaaa tcctcatatt
     7201 tggaaaaata gtacaaagat aattaataca atgttagaat gctttattgc aaaagatagt
     7261 aagataaaag atggagtcaa catacctact cttgtcttta aaacacttca tgatagatat
     7321 tttaaaaccc aatattttga tagctattat aattcactaa aatgtaaaaa gaaacttgtt
     7381 gaaattaata atgttcataa tagttactat atagatagtg agaaattttg ccaagaagct
     7441 tatgattggt ttgtagaaaa ccagtgatat aatttaaatg ttatatgggt ttctgattat
     7501 cttttca
//
LOCUS       DAGSHT010000164         7440 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.170, whole genome shotgun sequence.
ACCESSION   DAGSHT010000164 DAGSHT010000000
VERSION     DAGSHT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.170"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(281..1342)
                     /gene="ytvI"
                     /locus_tag="MJB72_002919"
     CDS             complement(281..1342)
                     /gene="ytvI"
                     /locus_tag="MJB72_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY8263994.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            1616..2356
                     /locus_tag="MJB72_002920"
     CDS             1616..2356
                     /locus_tag="MJB72_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBY8263995.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILRELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            2368..4146
                     /locus_tag="MJB72_002921"
     CDS             2368..4146
                     /locus_tag="MJB72_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8263996.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            4313..5230
                     /gene="pfkB"
                     /locus_tag="MJB72_002922"
     CDS             4313..5230
                     /gene="pfkB"
                     /locus_tag="MJB72_002922"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBY8263997.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            5267..7186
                     /locus_tag="MJB72_002923"
     CDS             5267..7186
                     /locus_tag="MJB72_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263998.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         3040 a          717 c         1288 g         2395 t
ORIGIN      
        1 tattccatat acaatagatt attcattaga gattgaagaa aaaataaaag aaaaatttaa
       61 aaatatagat gttttaaata atgaaaatga atataaagaa atgattgata ttatttgtgc
      121 agagtagaaa aaaagggagt gtcttagaat agacttttta gtttatgagg cactcttttt
      181 gtataaaatc aaatgtattt tcatcatttt aacaattaaa aagaacttat ccctatttga
      241 gaaagccctt tttaatttaa ataaaaatgt caaggttaaa ctatttaaat gcagtaataa
      301 tcccattatc atgtaagttt tttatgattg tagctgcgat agggaatcca aataatccag
      361 ctattccaaa tagttgagcc ccaacaaaca tacacattaa tgttattaaa ggatataggc
      421 caatttggtg tcctactacc tttggttcta atatttgtct aacaacggtt ataattatgt
      481 atagtattaa aagtcctatt gctaaattaa tatttccatt aatgagtgag ataatcatcc
      541 aaggtgtcaa aatacttcct gtacccaaga ctggcaaaat atcaacaagt gctatgatga
      601 cagatatagt aaatgcattt tcaaccttta ggatagtaag tccaataagt agttctatga
      661 atgtcactga tagtagtatt gcataggctt ttagtagttt taaaagagca ttaatactgc
      721 tattttttat atttattatc ataaattgac ttttttctgg aaattgtttt aagataaagt
      781 ttactatttt ttgatagtca aaagtaaaga aaaaagaagc aattaaagta ataataagtt
      841 ttaacataaa tgcaggtgtt ttacttgcaa tactagcaat agcattgagt gcatttgttg
      901 aaatagaagc tactaaagac atcatagatt gacttatatt atcccaacca agtattaatg
      961 ctaggttaac attaggtgta ctagcatcta tctttgaaaa taaagtattt agcgttggtt
     1021 gtatacttga tttatatatt tcaggaagtc taaaaaatac atcacttaca cttgcaaata
     1081 tcttcacacc aaagctaact aataaaaata tacacaaccc ataaaaaatt aaaagtatta
     1141 ctatagaaat aagtgtacga tttaaatttg tattatttgt aataagtctt ataataggtc
     1201 ttaagatgga tgcaatgata aaactgatta caaaaggcat taataatggc aataagaatt
     1261 ttaatacaat atatacaata cttgttatta aaactacata tgtaaattta attataaaat
     1321 tcttttgctt ttgaatatcc ataatataaa acctccaatg tatttgatat tattctaaat
     1381 atggtctttt ttacagaaaa tatactactg tatattgatt ataaactata aagcttatgt
     1441 aaagtaaata aattatgtaa aatttaggtt aattttgtat aaagagatac ttttatattt
     1501 tacaattaat aaccaaatac taaaaaatat tttaaataaa gaacttaaaa ttgtgataaa
     1561 attaatttat agatattaaa aaaagtataa tatatggttt ttaagaggtg ctaagatgaa
     1621 aaattttaga gatttaggtg ggaacaaaac tgaggatggc agaactgtaa agaagggatt
     1681 attttatagg tctgcaaaat tatcaaatct aagtgaaaat gatataaaaa ttttaagaga
     1741 gttaaatatt aaatatatat ttgattatag aagtgatgaa gaagctcgta aacatccatc
     1801 aacaataata tcaaatataa aaaatataag aataccagca atgagagagc tagaagaatc
     1861 aggaggaagt tttggttcta ttgaagatat gattgatgga ttgtttgaaa aagatggtgc
     1921 atttaatatg ttaaataata gttactataa tttgcctata aataatccgt catataaaaa
     1981 attagtagaa cttataagag actattctaa tttaccaata ttaaatcatt gtacagctgg
     2041 taaggataga actggtgtgg ggagtgctat aattcttatg attttagggg tatcgagaga
     2101 gaatataatg aaagattatc taaaaagtaa tgattttgca gataaagaga tagaaagatt
     2161 tatagaatat aagcctaaat ttaaggacat tccaaaagaa aatttgaaat atatatttgg
     2221 tgtaaatgaa gagtatatga aaactgcatt tagaagaatt gatgaagaat acattagtgt
     2281 agaagcatat ttatatggag aatttaattt aaataaagaa gaaataagaa aattaagaaa
     2341 tcaatatcta gagtaaaagg tgagtttatg agtttaaata taacagcaag acagataaat
     2401 ataataaaaa tattgttaaa ttcaaaagat acaattagtg gtattgcctt aagtcaagag
     2461 ataggatgtt cttcaaaaac tatacaaaat gaaataaagg atattaataa acaacttaga
     2521 aatggtaaaa ttttgtcaat aagaggagtt ggttataaac tagaaggaag ttttgatgag
     2581 gtaaatctaa atagcaatct ctatgatgat gtagatagag tagaatatat aatcagaaaa
     2641 atattaacct taagtaatga agaaaaaaat actataaaac ttgaagattt agccgattct
     2701 atgtatgtaa gtgtttcaac agtaaaaaat gatttaaaag aagtaaaaag aatgttggaa
     2761 aagtattcaa tatcagttaa atctaagcat aaacaaggta tatgtgtttt ggaaagtgaa
     2821 gataagatat taaattgtat agttgactta agcaataaga gagataatca attgagttta
     2881 aatgattttt taaatgatga tataaaggaa agaaaactat taataaaaaa attattatta
     2941 gacatattaa atgaaaatgg attagtatta actgatatag aatttaagag tgtgttaaat
     3001 aatatattag ttatgttaag cagacataag tataatgaaa aagaatttat agagaattat
     3061 atagaaaatt ataggagcaa aagaagctca attttagaaa atgaacaaaa caaagaaatt
     3121 ataataaatt caataaaaac attttgtaaa aatttaaaat tagctacatc aatagatata
     3181 agtaatgata aggtatttga agagttttta tataagcata ttagtagttt atgtaaaaaa
     3241 ataaagttag gtataaatca atcacctata atggctcaag atataaaaat aaaatatcca
     3301 tttgcttttg aactagctaa tatagctaaa aagacaatag aaaaagattt gaagatagaa
     3361 attaatgaag atgaagttgc aaacatagct ctacatatag gaggagcagt tgaaagagca
     3421 tcttataata ataaagataa ggttttcaaa actataatag tttgtacttc aggcataggc
     3481 acatcaatgt taataaaagc taaattagag aatatattta aggaaaaatt agaaatacta
     3541 aagattattc cttcatactt agttgaatat ataaaagtta tagatgttga ttttgtaata
     3601 tctactgttc ctattgaagt aggagatgtg ccagtaataa atatatctcc aattttaagt
     3661 gagaaagaaa ttagattaat tgaaaaatat atagaaacag gaaatatata cctagattta
     3721 gatataaaaa gcctgtttga tagtgaattg ttttttactg atttggtgtt taaaaggaaa
     3781 gaagaagtta tagattttat ggcaagtaaa cttgtacaaa agggctatat agatgaggat
     3841 atgaaaaaat cttactttga gagagaaaaa atagcaacaa cagaaatagg gaatatggta
     3901 actataccac atggagctat tggaaaaatt tttgataata aaatagcaat tggtatatta
     3961 aagaagccta tttcttggga aatatcagat gttcgtttaa taataatgtt agcccttgat
     4021 aaagataaaa tattagatta tgaagtaata ttttcaaaaa tatataaaag agtagattct
     4081 atagcaaaag ttataagtat atgtgaaaat agtagttttg aaaaatttgt aaatttattt
     4141 aattagatgg gtagtattgt atatacacct gtaaatttgg ataatataaa actaaataag
     4201 ataaaaacga taaatttctc ttttaattaa gagaaatttt ttgactttca aggatattaa
     4261 tataaagtta agatataaac ataaagcaaa taaataaaaa agaggtggca atatgattta
     4321 tacagtgaca ttaaatccat caatagatta tatagttaag ctagatgagc taaagacagg
     4381 aagtacaaat cgtgtaaaag aagaatacgt ttatccagga ggaaaaggta taaatgtatc
     4441 tcgcatactt aaggaacttg gaaatgataa tacatcttta ggttttataa gtggttttac
     4501 tggagaatat ataataagaa cattggaaga aaaagaattg aagacagatt ttataaagat
     4561 aaaaagtggt ttttcaagaa taaatgtaaa aataaaggcg ttaaaggaaa ctgagattaa
     4621 tggacaaggt ccaaatatag atgatgaaga cattgatact ttatataaaa aacttgataa
     4681 gctaaatcaa gatgatattt taatattggc aggaagtata ccatctactt tagatgaaaa
     4741 actatacgaa aatataatgg ctagattaga aaaaaagaat ataaaagttg tagtagatgc
     4801 aactaaaaat cttcttctta atgttttaaa atataaacct tttctaatta agcctaataa
     4861 cgacgaatta gaagaactat ttggtgttaa attaaattca attgaagaca tggtaaaata
     4921 tgctagaagg ttaaaggaaa tgggagctat aaatgtcttg gtatcaatgg gaaaagatgg
     4981 agcattgttg attacagagg aagatgaagt gcttataagt gatgttccaa aaggaaaagt
     5041 aaaaaattct gtaggagcag gtgattcaat ggtagcagga tttatatctg ggtatttgaa
     5101 tactgggaaa tatgattatg ctcttaaatt gggagcagct tcaggtagtg caactgcttt
     5161 ttcatatgac ttagccaaaa gagaatacat tgataaattg gtaaatgaga tatctgtaaa
     5221 gcagttttaa aatattaaaa aatatataga aaggtaatta atatttatga aaattataga
     5281 tttattagat gaaaagtcaa ttaagttaaa tttaaattca aaaaccaaat cagaagcaat
     5341 agaagaatta gtagatttgg tcgcaaatag tggaaattta aatgataaag aaaactacaa
     5401 aaaagcaatt ttggctcgtg aagaaatgag tacaacagga attggagaag gtgtagctat
     5461 acctcatgct aaaaattctt cagttactaa agcttgtata gcagcagcag tgtcaaaaga
     5521 aggtatagat tatgaatcgt ttgacggaag tttatcaaat ctatttttta tgatagctgc
     5581 accagacgga gctaacaata ctcatcttga agtattatca agattatcta caatacttat
     5641 ggatgaagac tttagaaata aacttataaa ttcaagctct gaaaaagaat ttaaggaaat
     5701 aatagataaa aaggaaagag aaaaattcag tgaagaatat caagatgaga aagttactga
     5761 aaaaaatata atagatacta aagagaatga tgctaataag tatcctaagg ttttagcagt
     5821 aacagcatgt ccaactggaa tagcacatac atttatggca gctgaaagtt taaataaaat
     5881 ggcagaaaac aaaggtgtat caataaaagt agaaactaat ggttcagcag gtgttaaaaa
     5941 taagcttaca aaagatgaaa tagaaaatgc aacatgtata atagtggcgg cagataaaaa
     6001 tgttgagatg gctagattta atggtaaaaa agtcataaaa actaaggtgg ctgatggtat
     6061 acacaaagcg gaagaactta tagacaaggc tgtaaatgga gatgctccaa tttaccatgg
     6121 aggagatgga agtcacaacg aatcaaatga agaaagtgaa agtggattta gaaaagtata
     6181 caaacactta atgaatggtg tatctaatat gttaccattt gttattggtg gagggatact
     6241 tatagcgata gcctttttac ttgatgatta tactattaac ccttcaaact ttggttcaaa
     6301 tacacctata gcagcatttt ttaaaggaat aggagataaa gcttttggat ttatgttacc
     6361 tgtacttgct ggatatatag catactctat atctgataga ccagcgtttg ttgtaggatt
     6421 tgtaggaggt gctctagcag gtgatggtgg ttcaggtttt ttaggtgcac tacttgcagg
     6481 ttttatagca ggttatttag tagaagggct taagaaaata tttagtgtac taccagcttc
     6541 attggaagga ataaaaccag tattgttata ccctttactt ggaacacttt tgatgggaat
     6601 tataatgaca tttttagtaa tacctccagt tacagcaatt aataatgcaa tggttggatt
     6661 tttaaatggt ttaggtggaa cttcaaaaat agttttagga ttagtattag gtggaatgat
     6721 ggctgtggat atgggtggac ctgtaaataa agcagcatat gtatttggag ttgcatcact
     6781 tgaatcaggg cagtttgaaa taatggcagc agtaatggct ggtggtatgg ttccgccact
     6841 tgctatagct cttgcaacta cattttttaa gaatagattt actaaagaag aaagagattc
     6901 tggtaaagtt aattatgtaa tgggattatc atttgttact gaaggagcta taccttttgc
     6961 agctggagac ccacttcatg ttataccagc ttgtgttgga ggttcagcag tggcaggagc
     7021 acttagtatg ttgttcaacg cagctcttcg tgcacctcat ggaggagtat ttgttatacc
     7081 agtggttacc catccatttg catatatatt agctatagct gtaggtgctt tggttggaat
     7141 gatgctttta gcattactta aaaaaccatt aaatcaagaa gtatagcaat taaaggatat
     7201 tcaagaactt aaaatatgat gttttataaa aattaatata ttgctgaatt attttaaaat
     7261 aaggatttta tgaggctgtt atataacgat ttgtaattgt tatcttgaca gtcttttatt
     7321 tttatataag taaaataagt gtgttttgct ttatttgtat ttaaattgtt tttaagttag
     7381 aatataaaga aaattatata ttattttaat attcactcga actagaaaat aatatttgtt
//
LOCUS       DAGSHT010000165         7433 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGSHT010000165 DAGSHT010000000
VERSION     DAGSHT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.171"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..932
                     /locus_tag="MJB72_002924"
     CDS             <1..932
                     /locus_tag="MJB72_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8263999.1"
                     /translation="KKNHKIINPFSVKISQQYPTVFNLSIQFSKIIENEYHIKISQDE
                     IGFIATHIAVPFEKREEANFNKKYKIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSL
                     LEEKAVIEFAPDLIFSITNLLFEINAPVILINEILDELDYLDIKESVRFADHIGNISS
                     PKQYILGLFDKNHFRCIKDKAKYKDILDSMSKCIVDEGACSPTYPKDVWERESYLSTI
                     YTNGVAIPHPIEMTGNKNIISVALIQTDIIYENRVPKIIFMISLIKGNLELHKQISKY
                     LSKIMANKDMVDMLNKSQSYEEFMYKLKIYLGG"
     gene            935..1978
                     /gene="ypdE"
                     /locus_tag="MJB72_002925"
     CDS             935..1978
                     /gene="ypdE"
                     /locus_tag="MJB72_002925"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY8264000.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            1993..2298
                     /locus_tag="MJB72_002926"
     CDS             1993..2298
                     /locus_tag="MJB72_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY8264001.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            2395..2730
                     /locus_tag="MJB72_002927"
     CDS             2395..2730
                     /locus_tag="MJB72_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8264002.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            2762..4084
                     /locus_tag="MJB72_002928"
     CDS             2762..4084
                     /locus_tag="MJB72_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8264003.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            4094..5164
                     /locus_tag="MJB72_002929"
     CDS             4094..5164
                     /locus_tag="MJB72_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY8264004.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            5169..6356
                     /locus_tag="MJB72_002930"
     CDS             5169..6356
                     /locus_tag="MJB72_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY8264005.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(6553..7425)
                     /locus_tag="MJB72_002931"
     CDS             complement(6553..7425)
                     /locus_tag="MJB72_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBY8264006.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
BASE COUNT         2819 a          853 c         1332 g         2429 t
ORIGIN      
        1 taaagaagaa ccacaagata ataaatcctt tttcagttaa aataagtcaa cagtatccaa
       61 ctgtttttaa tttatctatt caattttcaa aaatcataga aaatgagtat catataaaaa
      121 taagtcaaga tgaaataggg tttatagcta ctcacattgc tgttcctttt gaaaagagag
      181 aagaagctaa ttttaataaa aaatataaaa tagcaataat atgctcttct ggtggaggta
      241 gtgcattttt gataaattta agacttaaag aaatttttcc aaatgctgag ataagaaatt
      301 tttcattact agaagaaaaa gctgtaatag aatttgcacc agatttgatt ttttcaataa
      361 caaatctttt atttgaaata aatgctccag ttatacttat aaatgaaatt ttagacgaat
      421 tggattattt agatattaaa gaaagtgtta gatttgcaga ccatatagga aatatatcaa
      481 gcccaaaaca atatatatta ggtctatttg ataagaatca ctttaggtgt ataaaagata
      541 aagctaagta taaggatatc cttgatagta tgtcaaaatg tatagttgac gagggagctt
      601 gttctccaac ctatcccaaa gatgtatggg aaagagaaag ttatttgagt actatttata
      661 caaatggggt ggctatacct catccaatcg agatgacagg aaacaaaaat ataatatcag
      721 tagctttgat acaaactgat attatttatg aaaatagggt gcctaaaatt attttcatga
      781 tttcattaat aaagggaaat ttagaacttc ataagcaaat atctaaatat ttaagtaaga
      841 ttatggcaaa taaggatatg gtagacatgc taaataagtc acaatcttat gaagagttta
      901 tgtataagtt aaaaatctat ctgggaggtt agacatgaat atagagtttt ttaagaggtt
      961 gtcagaagca gatggtatag catctaatga agaagaggtt agacatgttt tattggaaga
     1021 actaaaagct tatagtgata aaattatctg tgatgggcta gggagtatca tcttttctaa
     1081 aataaaagat gagagtgcac caaacgtaat gatttgtgct catatggatg aagttggatt
     1141 tatggtgaga agtatagaca aacttggtat gattcactta ataacaatag gaggagtaaa
     1201 acctcttgca cagtttgtac aaaaagttag aattacaaca aaagaaggga agaaaatccc
     1261 cgctgtcata aatgctacat ataataatgg taaggctgaa aatatatatg cagatattgg
     1321 agcatataca gaagaagatg tatataacct agggattaat gttggggata tggtaacata
     1381 tacaacaagc tttgaggaat ttactttacc agaccgatta gtgggaaagg cttttgatga
     1441 taggatagga tgttttgtga tgggagaagt tttaaaagaa cttaggaaag aaaatctaaa
     1501 ttgtaatatt cactttgcgg ctacaagtag tgaagaggtt ggaataaggg gagcaaagac
     1561 atcaacacag ttaataaatc cagatatagt atttgtaata gatgtagcat gtgctaaaaa
     1621 tgagtttgtg agagactata caaataagaa acaaattgat aaaggtgtaa tgctaatgca
     1681 tagagatagg actctagtcc ctagtagaaa aatgatagat tatgtaatgg aaattgctga
     1741 taaaaataat ataccacttc aacatgatat gtttgaatca ggtgggactg atggaggaga
     1801 agcacatctc gtaaatgaag ggaaaccttg tgtagtaact tgtgtaccag ttagatatgg
     1861 tcattgtgca ttttcaatag taagtaataa agatttggag aacattataa gattgtatac
     1921 acagttaatt ttaaattttg atgaagaaca atataaacat ataagaaatt ttatctaaag
     1981 gagaataaga aaatgtcaac agaagtacaa ataatggaac ttatatcaac tgcaggagag
     2041 agtaaatcga aagcttttga ggctttaaaa aaagtgaagt caaaagattt taaaggagca
     2101 aaattgttaa tagaggaggc tcgtaaaata gatattgaag ctcacaggat acaaacagaa
     2161 ttaataactc aagatttaaa tgaagatgtt gaaagtccag caatgagtct tttaatggta
     2221 catgctcaag accactatat gacttctcag ctagcaaaag acttgataga ggctttgata
     2281 tattgtcttg ataagtaatt gtaatataga aatagattat ttatgtaaaa attcttttaa
     2341 aagtttagat gcttttaaga gataataaaa atacaaactt taggaggaat tattatgaaa
     2401 atattattat gttgtgcagg tggattttct actaatatgt taatgcaaaa tatgaaaaag
     2461 gtggtaaaag agagtgctaa actcaatgaa aaagattttg agtttacagc tataccagca
     2521 gattcaatag aaagtgaagt tgatgattgg gatatagtac ttataggtcc tcaaatagct
     2581 cacaaaatag acttcttaaa aggaatatta gagccaaaaa ataagccttt tgcagtaata
     2641 gataaagatg tatatggaaa aatggatgga gctacagtta tgaagttggc attaataact
     2701 tataagaaac atcaattagc tcaaggataa taaaaataat gagggaaaag ggagattaga
     2761 tatgttttca aaatttgaag catttatgaa tagagtattt atgcctttag ctcataaggt
     2821 tgacaatcaa cgtcaccttg gttctataaa ggctggtatg gttgctatga caccattcac
     2881 aatattagga agtatatttg caatacttcc agcacttcca aatatgcttg gagaaaataa
     2941 tccaataagc aaattcatac ttagcaattc agcaatattg gatttaccag taagtctttc
     3001 cataggttta ataggattat atgcttgtat gtgtattgct tttaatctgg ggaactacta
     3061 taaattatat ataccaggtt gtgttgcact atcaactttt tcttttttat tcctagtagc
     3121 ctcatttaca ccagatggag ctttagaaat aggaaattta ggagctaaag ggctatttac
     3181 aggtatgata gcagggcttg gttctgtgga gttatataat ttttgtaata agaagaatat
     3241 cacaataaaa atgccagaaa atgtacctga ttttgtatct aggtcttttg aattaatacc
     3301 tactactata atagtttgtg gaacttttat agttgctcgt tttatttcat taaaagtatt
     3361 tggagaatta cctccacaga tacttactca atttttatct ccattagtag gaagtatgga
     3421 taacccatgg tcagttcttg cacttaactt tgctatatgt actatattct tttttggaat
     3481 acattcatca gtatttagtc ctataactag acctataatg attacattta tagctgaaaa
     3541 catagcagct atgcaggctg gagaaacaat acctcatttc tttacagcag gagcttcaag
     3601 tgcattcttt ggatttacag gatgtggaat tagtataggt tgtgtaattg catgtatgtt
     3661 atcaaaaaat gcttcctata agaagattgg acgtgtatca ttattccctg cattatttgg
     3721 gataaatgaa ccaatattat ttggagcacc gataatatta aatccaataa tgtttatacc
     3781 acatgtattt ggtggagcta taataggtac actaccaatg ttttttatgc actggggatg
     3841 gttagcaaag ccagtatttg accctccata tgtaggggta ttcttagaag gatttttaac
     3901 taatggagat tggcattcta tattagcaaa tgcattgcaa ttagtattag cagttttatt
     3961 atattggcca ttctttaaaa taatggaacg tcaacaaaat aataaaaaac aaaaagaaga
     4021 aactaagaat atattctcta aagaagaaga agacttattg gatgatttag gattggattt
     4081 ctaatatttt gttatgaata atacaatgga gagattagta tccttaatct ataaaagagg
     4141 tgttgacgct cttttgatta aagcaaaaaa tactaaaaga tatataggag ctttatcagg
     4201 tagtggtgta tatgtgcttg ttactaagga taaaaattat caaattctag atggcagata
     4261 tattgatgaa gcagataaaa aaacgactgg tttcataaaa aggattgtat ctcaaggttc
     4321 ttatatacca actataattg agttattaaa agaattaaat ataaaaaagt tagcaataga
     4381 aaaccaagct atgagtatac aagagtatct ttcactaaaa aatgaaggtt ttgaaatcaa
     4441 tttaataaca aatgaattat ggaagctaag ggctataaaa tctaaagaag aaattaaact
     4501 tattaaaaag gcttgtgaga ttacagatga agtatttgat gaggttattt ctgaaattaa
     4561 aaaagatatg actgagctag aagtatcagc tctaattcag tatcatgcat taaaaaaagg
     4621 tgcaagtgga atgtcttttg aaacaatagt ggtatcagga gaaagaggtg caatgccaca
     4681 tggaagacct acaaacaaaa aattgaaagt gaatgaagct ataacaatag attttggtgt
     4741 tgtttatcaa gggtatcaat cagacatgac tcgtacaata tctataggca aacctcctaa
     4801 aattattaaa gagatatatg atgtagtatt ggaagcacaa ttatctgcaa tagagtcaat
     4861 aaaggaagga actagggcaa gtgatgttga caaagtggct agaaaaatta tagataaaca
     4921 tggatttggc gaatatttta atcatggact gggtcatggt ataggtcttg gagatggtga
     4981 agttcccact ttaaatccaa atagtgaaga tatattagtg gaaggaatgg taatgtcttg
     5041 tgagccaggt atttatattc caaatgtagg gggagttaga atagaagatg atattgttat
     5101 aatagatgga aaaggtgttc cactcaataa gacatccaaa gagtttataa tattaggaga
     5161 gtaaaattat gaaatataat tttaataaag tattaaatcg taaagaagga aattgtagaa
     5221 aatggagcaa ccatgtgatt aaagaaaaat ttggcttaaa tgaagatgca attcctatgg
     5281 atttagctga tatcgatttt gagtgtgctc ctgctataaa agaatctatc ttaaaaagag
     5341 catcagtagg agattatagt tatactttta taggagatga cttctatgat gctattataa
     5401 attggaataa aagaagattt gatgtatata tagaaaagga ttggataaaa ttaacttttg
     5461 gtactgtttc aacccttcat tatatagtcc aagcatatac taaagaagga gagggtgtat
     5521 tgataaatac acctgcttat gacccttttg cagaggctgt agtaaataat aatagaaagt
     5581 tgtgttgtag ctcattaaaa ttagataaca atagatatta tttagattta gaggatatgg
     5641 aaaatcaaat gaaatacgag aatataagat tgcttatatt ctgtagtcca caaaatccat
     5701 caggtcgtat atggacaaaa gaagaactat atcaagtgtc agagttatgt ttaaagtata
     5761 atgtaatttt ggtaagtgat gaaattcata gagatataat atttaaagat tatagatttg
     5821 tatctttgtg gaatgctcat ccagaaattt ataaaaatag tataatctgt gtatctccta
     5881 ataaaggctt caacttaggt ggattaaaaa catcatatgt attaataaga gatgtaaaaa
     5941 ttagggagac tttattagaa aggctaaaaa gtaattcaat aacttctcca aatgtatttg
     6001 caataccagc aatagttgca gcatataatg aaagcgaaga atggctagat gctatgacta
     6061 gttatgtaga ggagaatttt gaaatagttt atgatttctt tgaaactaat ataccaaaag
     6121 ctaaagtgat gaagtcagac tcttcttttt tagcctggat agatgtaaga gaagtattta
     6181 gaaatgaaga agaatctaaa gaatttttta gacatgcaaa cttaactatg gtagttggaa
     6241 gttattttgt gaaagatgga gatggattta ttcgtattaa tgtggggtgt cctagagaaa
     6301 cattaaatga ggctctaaat agaatcaaaa aaacgtatat aagtatgtat tgttaaatca
     6361 taaaaaaagt atctatggtt tatataaaaa cacattattt agttatcaaa tattactgga
     6421 attttgtatt ccagtaatat tttttgatta aggaatatta ttgaggaata ttaaataata
     6481 ttatttcata cccttatatt aacttatata aaaatatttt attattttgg gaacgggtta
     6541 gctaaccttt atctagtgta tatttatatt gtaagaagca gtaaaaactt ttcctttact
     6601 aagtttatta ataaacttct tatctttata aattttgtta gaatttatac tatcagcaag
     6661 accataccaa ggttctatac ataaaaaagg agcagtagaa ttagtttcac tataataagg
     6721 agtccaaata ccaactagag ggaaatcttc catagatata gttacatatt tactaccatc
     6781 attattacat atagacactt catctatgtt ggtgtatatc aaagcatcat tcttgaatac
     6841 ttctggattt aactgtaagt tttttaattt tccaataggt attatatcat ctgtaaaaga
     6901 accatttaaa ttgatttttt ctacatcatt tttactttta aactcaaggt agtgtttaga
     6961 aaaatcattt tgttcgtaaa aaggaaggtt gaatgcagga tgtccaccta tagaaaagaa
     7021 tatatcttta gaatcagtat tttttacaat ccattctacc tcaatactat tatcatttaa
     7081 tgtatagttt atgattagct caaaagaata tggatacttc tttaaagtag attcattatc
     7141 tgttaattta taagttacaa atgtatctcc tttatctaca atctcaaagt ccatatctct
     7201 agcaaaccca tgttgagtca tgctatataa attttcttct attattgttt cattatcaaa
     7261 aagtttacct actataggga acaatatagg tgattgtcta ccccaatatt ttttattacc
     7321 atcccaaaga atgtttttgt cgtatttttt agaaaatatt ttagttagtt cagcaccaaa
     7381 actgcttgat tcgataatta aattttcatt ttgtaatata ttcatttaaa atc
//
LOCUS       DAGSHT010000166         7432 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGSHT010000166 DAGSHT010000000
VERSION     DAGSHT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.172"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            343..513
                     /locus_tag="MJB72_002932"
     CDS             343..513
                     /locus_tag="MJB72_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264007.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            1056..1934
                     /locus_tag="MJB72_002933"
     CDS             1056..1934
                     /locus_tag="MJB72_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBY8264008.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            1970..3865
                     /locus_tag="MJB72_002934"
     CDS             1970..3865
                     /locus_tag="MJB72_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8264009.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            4133..6040
                     /locus_tag="MJB72_002935"
     CDS             4133..6040
                     /locus_tag="MJB72_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY8264010.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            6030..6479
                     /locus_tag="MJB72_002936"
     CDS             6030..6479
                     /locus_tag="MJB72_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8264011.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            6579..>7432
                     /locus_tag="MJB72_002937"
     CDS             6579..>7432
                     /locus_tag="MJB72_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8264012.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRY"
BASE COUNT         3064 a          739 c         1290 g         2339 t
ORIGIN      
        1 ttaagtttgt taaaaatatt tatataatat aatatatgat taaggtcaga ttataattgt
       61 tttaagataa aatgtaataa aatttaatgg agaaatttta taggtagata aattttaata
      121 tttataagat tatgttaagg aaacacttaa aaattagatt aaaattgtgg gtatttaaat
      181 caactaaaag atattgacta tttagtcaat atcttttttt attaaaaagg aatatacata
      241 taaataggtt agattaaaaa ttgggataaa tatgatttat atagcatcat ttaattaaaa
      301 ggagctatat tagtattcat tcatacaatt ggataatata caatgtcagc cttgctttat
      361 ggtgtttgta ataaatctat ttcagtttct attgtaatta atatgcaaga atttaagata
      421 ggagtatcta tggcttatgg tgtattagta attggtatat gctatatagc attaggaata
      481 atttttaaat tagatagaaa aagatttaca taagataaca aaggctaatt tataaattgg
      541 tgcttagaaa accaaatcac tttattagct agagatgaat ttggagggtg tcactttggt
      601 gttttatgta aagtttatgg aagtaaaaca ttagataatc gtgagtaaga tttctataaa
      661 tcgtatatac tattggaatc caagaaaagg tattggccaa atagctaata tctttttaat
      721 ttttttgact ctaatatagc attaactatt gcatgagtat ctttaaattt atagttaatg
      781 tatcttaatt gtttattata aaatctagga aggctaaagc ataaaacact taaaaagaga
      841 agatgtacat gagaaatata attaactatg atgcagactt ctaaaaatta tttagtgaaa
      901 aggaacttag agtgtataat aaaagttcct tttcactaag gaaaacgatt gttattttct
      961 gacattcatt accctgatat tatttaaata taaacaacaa caaaattaac aagaagcaaa
     1021 ttcaacctaa aatttaaaat atcgggaggt acaatatgtt aataaacatg aaggaaatgc
     1081 taaaagtggc acaagaaaat caatttgctg taccagcatt taatatagga agtgggcaaa
     1141 tacttaaagc agtagtacag agtgctaacg aaaaaaatgc accagtaata cttgcaatac
     1201 acccaaatga gctaagtttt ctaggagata gctttgtagc tagttgcata gaagaagcaa
     1261 ataagagtaa agtacctatg gtaatacact tagatcatgg ggaaaataaa gaacaaatat
     1321 taagggcaat aagatgtgga tttacatcag taatgattga tggctcacat ttaccatatg
     1381 aagagaatgt agctatatct agggaaatag tagaaatagc aaaaggatta aatgtgtccg
     1441 tagaaggaga acttggaaca ataggaacaa caggaacatc aagtgaaggt ggtactgatg
     1501 aaataatata tacagattca aaattagcta aggattttgt tgaaaaaaca ggagtagaca
     1561 cactagctat tgcaattggt acagcacatg gtatatatcc aaaaggtttt aaaccagagc
     1621 ttaaactaga tttattaaaa gaaataagag aagtagtaga tataccttta gttcttcatg
     1681 gtggttcatc aaatccagat gaagaaatag cacaagcagt taaactagga gtttgtaaaa
     1741 taaatatttc gagtgatgtt aagtcggcat attataagaa gtgcagagaa ctattagaac
     1801 aaaacccaag cttatatgag ccagatacta tatatccacc atgtataaaa gctgcaagag
     1861 aagttataga gtttaaaatg aatctattca atgctataga taagttaaag tgcttttata
     1921 aaaataaaaa ttaattatga ggtaacaaat taaaaggagg ggagaattta tgataggtaa
     1981 aatactacga agcgattgta ttgtactaga taaacaatta caaacaaaaa aagatgtgat
     2041 taattatatg gcaaatcatt tgtaccatgg aaattatata aataataaag aaaagtttat
     2101 agctgatatt aataaacgtg aaaaagaatc atcaactaat atgggtaatt taatagctat
     2161 acctcatgct aagtcaaatg tagtaaatca acctgtaatg atgtatttaa gaacagatag
     2221 agagattaca gataatgatg gtcaaaaact taggtcattt tttatgatag cagttccaga
     2281 atcaggttca gatgaacatc ttaagattat atctaaacta gctacatatc taatggaaga
     2341 aaattttctt aagcatctac taagtgcaga aactccatac gaaatattaa gtatatttaa
     2401 taataaagct gcagaatcag gagattttag taataatcaa aataaaggtc ttatagttgg
     2461 gataacagga tgtccaacag gtatagctca tacttttatg gcaaaaaaag cattagagga
     2521 agcagggcaa gaacttggat atgaagttaa aatagaaact catggagcta taggtgtaga
     2581 aaataagttg actaaagaag atataaaaag agctgaatac gtaataatag cttgtgataa
     2641 agaagttcaa ctgagtagat ttataggaaa gaaagtgcta caaaagaaag tagtagatgc
     2701 taagaatgtt tcattagcta aaaatttaat tataaacgct gaaaatggaa tggctaaaga
     2761 aattaaacca ggtggaaaga ctttagaaga ttcaaaagaa atgcttcaag ggtcagagaa
     2821 aaagggtgtt tataagcact taatgagtgg tgtatctgaa atgattccat ttgtagtgat
     2881 aggtggaatt ggtatagcca tagcttttat gtttggtata tttgcatcag acccaactca
     2941 tgaaacatac aaccctatag ctggattctt tggacaactt ggaggcgatt cagcatttaa
     3001 gttatttata ccgatacttg ctggttttat agctaaatca atagcaggaa gacaaggatt
     3061 tgctcctgct atgatagctg gttttatggc agttattgga ggttcaggtt ttataggtgg
     3121 tatggtagct ggttttgcaa caggatatat agctttagga gtaatgaaga taacagaaaa
     3181 tttaccagat gtattaaaag gagtaaatgc tgtattgata tgtccacttt tgattgcatt
     3241 tacgagtgga gctataatgt tctttgtagt taatacgcca gcatcatggt taaatgaagc
     3301 tttacaatct tggttacaaa gtatgtctgg aactaataaa gtattattag gtgcaatatt
     3361 agcagggatg atggcatcag atatgggagg acctattaat aaaacagctt cagcttttgg
     3421 attggctatg ttttcaagta atatatttga accatcagca gcattaatgg taggaggaat
     3481 ggtacctcca ttagcaatat cattagctat gttaatattc aaaaataagt ttactaaaga
     3541 agaaagaaag tcatctctac caactgctat aatgggggca tctttcataa cagaaggcgc
     3601 aataccgttt gcagctgcag acccattaag aatgattcca gcaaatataa ttggttcttg
     3661 tataggtggg gcaatatgta tgagtttaga tataacttta atggcaccac atgggggaat
     3721 atttgtaata ccatttgcat gtagtgcacc agttgcatat atagcttgta tattgattgg
     3781 tacagtaata acagcattga ttataggggt aacaaagcct actataagga gtgaaaatag
     3841 tgaagttgaa ttacttggtg cttaaattat aaataatagt aaaaataatt aatggaataa
     3901 caacaagtat atagaataat aataaggata tttagaatca gttaactatt tctaaatatc
     3961 cttaattttt attttgaaat tatattatct attatatcaa tttagtaagg tgtatcataa
     4021 attcttttat gatacagaag tatcttagta attttacata gttagtaaat atgttaaaat
     4081 agtacttaat ataaacatag aaatatataa acataggtag ggagggctaa gtatgcttgt
     4141 gtcgaggcaa attgaatttc ttaaattgat actaaacgaa aataaatata gacctataaa
     4201 atactttaaa gataaattaa atgtatctga taaaacttta caaaaagatt tgaagatgat
     4261 tgagaaatat ttagaaactt ttaatataaa aattgatata aaaagaggct atggaatatt
     4321 aatagaagat ttagcaaaga agaacataga cttaattaat agtttaaata tacaatcgaa
     4381 ggaaaataaa tctttatcag tagagcagag aagaatggag attttaaagt atttattgct
     4441 aaattcaaat aatataactt caataaatca gttagcagat aaatattatg taagtaaaac
     4501 ttctatagta aatgatttta aatatataga ggcatggata aaagaataca atttaaaact
     4561 taataaaact ttagaaggaa ctaagattat aggtaaagaa gtggatataa ggaaatcgat
     4621 tgccaaaatg atggatgatt tattagatga aaattatgaa caacatgata tatctgagtt
     4681 aacaagatta gattctgcta catttagtgc attgataaat ttatttgata ttgattctat
     4741 tatatttgta gaaactataa ttacagaatt agaatacaat ttaggatata ctataaatca
     4801 gccctactat ataaatttaa ttacccatat acttatatgt ttaaagagaa tcgaagaagg
     4861 taatcaaata gaatctcagg aagaaagaga aatttctgta gataatttgg acgagttagt
     4921 atataaaaat gtcactacac ttatagataa aatagaaaat agatatagag taaaaataac
     4981 gaaagatgaa attatgtaca tatatatttt tttggtatca tctggattta gtagcgaaaa
     5041 taatagtagt tacaatgatg aatcagatag attaagtgaa agtgaaaaag tttcatctat
     5101 aatgatagag aatatgtcac gatttttaaa tataaatctc aaaaatgatg acttactaca
     5161 gaaaagtcta gcatctcatg taaggcctat gttaaacaga ttaagatatg atatccaaat
     5221 aaaaaacccg ttattaggag agatacaaga gcgattttca gatgtgctgg gattatgtat
     5281 gatgactata aatattatga cagagtatga taatctgaag aatattagta tagatgaagt
     5341 ttcgtatctt gccacatatt ttcaagcggc aatagaaaga aatatgagta gtaaaagagt
     5401 aatagtagtt tgtcatacag gatatggaac atctcagctc cttgcagcca gattaaaaag
     5461 agaatttcct gaatggacta tagttgatat agttcctatg catcagttga gtaagcgaaa
     5521 tttagatgat gtagactttg taatatctac tgtaaactta gatttgaaag ataaattaca
     5581 tatagttgta tcagtactat taatggaaaa tgatattaat aatataaaaa atgctttact
     5641 ttctaaacct aaaaaaagta tgaacttaga tacaaactta ttaggaatgt atttagaaga
     5701 taatatatat tttaattttg atgtagctca gatggaaagt ttaataggtg tgaatttagg
     5761 gagtaaatat gaatctattt cattaggaaa agatttaaat atatatatac ataagcaaag
     5821 taaattaaat aaagggataa tgaatatcaa taatgttact agaacgattg atatttattt
     5881 agaaattagt aatcaaagtt ttttaaagaa tattttaatg gaaatatcaa atttatatag
     5941 gcaaaagaac tatataaact atataataga ttgcaagagc aaagaggata ttaaaatatt
     6001 atttaagaat aatctattgg agaaaacaaa tggaaattag agaaatatta acagaagaaa
     6061 gcattatttt agatttgaaa tcaactacaa aagaaggcat aatacaggaa ttagtagata
     6121 aattatacga tacaggaagt ataactttaa aaagtaagtt tataaaggca ctaaaacata
     6181 gagaaaaaat aggctctact agtataggtt atcaaatggc catgccacat ggaaaaacaa
     6241 aatatgtaaa gaaggcatcc atagtaatag gtaaatctaa aaaaggtgta gattggccaa
     6301 gtatagatgg cttacctgta aaggtaataa ttatgtttgc tatgccacaa tctggaggta
     6361 atgaagatat gatattaata tctaaaatag cttacatttt aggtggtaaa aatataggtg
     6421 aaagactttt aaatgcacat acaaaaaaag aaatattaga tatattttat gaagtgtagg
     6481 tattgtataa attataaatt tgaggttttt aaaataaaat gtgtattctt ttaaatagta
     6541 tagattaatt tttagacact agagaggaga ggatatatat ggcaatatat ttagaaatgc
     6601 ctaaattaat acatgaattt ccttttcgta gtctatcaaa tgaaggagaa gtattaacaa
     6661 caccacactg gcataaggaa atagaaatat tacttataac agaaggtgta gtaaatctat
     6721 ttataaatga caagcctatg caattaaaaa aaggcgagat agtgataata aaaggaggag
     6781 atattcatta tatactgcca tcaccgggaa gtgaaagatt agtatttcaa tttgatatat
     6841 ctttttttaa tgatttaatt ttattaaatg aggagaaaga aagcttaatt aatttattca
     6901 gtagtataaa aaaatgtagt agagattggt cagcaaaaat aagaaagtca gttttcgata
     6961 tattgataga tatctacaat gaagacctat acaaaataga aggatatagt tatgcaataa
     7021 aaggtaaaat gtataattta attcctatat tatatagaca gattcctaga gaaattgaag
     7081 aaaatacagt agaatatgaa ataaactcta aagagatatt agagagattg aacaatatat
     7141 tcaaatatgt agaaaaaaat tataaacaac ctataaaatt agaagatgtg tcttatgaag
     7201 taggatttag tgtatattat tttactaaat ttttcaaaaa aaatacagga aaaacattta
     7261 taactttttt aaatgaatat agattagaaa aagcaaagtg gatattactt aataacaata
     7321 tatccattgt agatttggtt gaagaagttg gttttggaag tacaaaaact ttttacagag
     7381 tatttaaaga aaaaattgga gtctctccat tagaatttaa aaaaagatat aa
//
LOCUS       DAGSHT010000167         7393 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.173, whole genome shotgun sequence.
ACCESSION   DAGSHT010000167 DAGSHT010000000
VERSION     DAGSHT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.173"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1736)
                     /locus_tag="MJB72_002938"
     CDS             complement(<1..1736)
                     /locus_tag="MJB72_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBY8264013.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKY"
     gene            complement(1884..2675)
                     /locus_tag="MJB72_002939"
     CDS             complement(1884..2675)
                     /locus_tag="MJB72_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8264014.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(2711..3367)
                     /locus_tag="MJB72_002940"
     CDS             complement(2711..3367)
                     /locus_tag="MJB72_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264015.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(3360..4325)
                     /locus_tag="MJB72_002941"
     CDS             complement(3360..4325)
                     /locus_tag="MJB72_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBY8264016.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(4431..4537)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4827..5771)
                     /locus_tag="MJB72_002942"
     CDS             complement(4827..5771)
                     /locus_tag="MJB72_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBY8264017.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            5882..6349
                     /locus_tag="MJB72_002943"
     CDS             5882..6349
                     /locus_tag="MJB72_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264018.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(6519..7118)
                     /locus_tag="MJB72_002944"
     CDS             complement(6519..7118)
                     /locus_tag="MJB72_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264019.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2413 a         1201 c          758 g         3021 t
ORIGIN      
        1 ctatattttt gtgttgtaac tcctaataca tcactagaaa ttccgaacaa ctctctaaag
       61 gctctatttg tatatagtac tcttgatgta cttaaatcct caagaaaaat agcctctggt
      121 ataacatcca gtaatttttc atatattatt ttttgttcat ttactgttct aagaatattt
      181 atctctttag ttatatctct aaatgtaagc aatttaaaga gtttattatc tgaccaatag
      241 tctccaactc taacttcttc aacatgttct tcaccttctg agtcagacat cacaaaatta
      301 actatacagt ttgctgtgtc attgtaactt actatttcag ctttcttttc ttgtgttaga
      361 tttaatcttt taaattccct attgtcaaag aaatctatgt taaaagtttc aaaatattgt
      421 tctaatgtca tatcgactaa atcacttgaa acacatttaa aactcttttc atatttttta
      481 tttatactta ttaccttatt cttctttatg ccaccttcag cacttcttac aagtatagga
      541 gatggaatat ttagtataat gttttcatag tctttattta tatttcttat ttcttcatct
      601 attttgattt catttgtaat atcatttaaa cttattaagt aattgctttc gtctttatta
      661 ttacttaaac ttaacaaata aattttatca tttaactcta ccagtttatc aattatactt
      721 atatcgttat caaatttctt caatttttca gcaacttttt catcttcctt tataaaagtt
      781 aatattttat aataattatt catatccttt gaaaattcat ctttaaattt attatttgtg
      841 taacttatat taaaatcata gtctgttact atccatacat ttggtattgt atccagagct
      901 tgtctaagta attcttttct ctggtctatg tagtttttta atgttctttg tataatcata
      961 tccttttcta ttgctaaatt tatctttcct aattgctctg actttttact taatttttta
     1021 ttgattgaat caagttcata atatggatac actactctat tattaagact gtatacatat
     1081 acataataag tatggttaca taatagtaaa tatattactc ctattaatct atctgaatct
     1141 actatattaa tcttagaaat tgctacaaaa catataatag ttgtaaaaaa aacaaaatat
     1201 gttaaatctt ttattaactt atagtgctca atgttactat caagtttatt caacaaatat
     1261 agtaaattaa acatagatat acaaatatta attaatataa taatcatatt ccagttaata
     1321 tcactaaaaa tatttcttat aaagtaatct aatatcacaa aagcactata aaaaattagt
     1381 attgggctta ttcttagttt ttttacttta gaaatggtta agtatataaa agtagacata
     1441 ttaattatgc ttaagttaag caatacaatt tggaattttt gaatttttaa atttactact
     1501 cctaaaatac ttacaaaaac taatacaaga caccctactt tcaatatatt tataaattca
     1561 tctgttttaa agctacttat ataaactata gaatatccaa atatccaaag taaaattatt
     1621 ctatacatat ttatagaaac tgtatacaaa tttatattta aaagtgcaat aaccaaaaat
     1681 aacccattag ctagtatata gaccttattc ttacttaaag ttcctttaga ttccatattc
     1741 cccaccactt cttcattttt atatagtttt acctataaat atgttaccac aattcttcca
     1801 aaaaatttgt cactttctag ttataaaaaa aaatgagtaa acatttttaa ttttactcat
     1861 tttgtagatt ttgtatattc agtttaaaaa gctggaacta tacttccttt atattcatct
     1921 tgtataaatt ttttaacttc tttactattc atagcctctg ataaagcctt aatcttatca
     1981 ctatctttat tattttctct acaagctatt atatttacat atggagaatc acttgattct
     2041 attactattg catccttagt aggatttaag ttagctgtta aagcataatt agaatttatt
     2101 actgctccgt caacatcttt taatactgtt ggtaattgct ctgcattcat ttcttttatt
     2161 actatatttt taggattttt agttatatct tttttagtta taagctctcc atcttttact
     2221 tctatcaatt tattttttgc taataattta agtgctctag ctccattagt tgcatcattt
     2281 ggcacagcta taacagctcc atcttttaat tcatctaatg acttaacttt ttctgaatag
     2341 aatcccattg gctctatatg aactttagat gtataagtaa gtttatatcc tttctccttc
     2401 acagtttcct ctaaaaatgg aatatgttga aagaagttag catccaaaga cccttcatct
     2461 aaagctgtat ttggaagtac ataatcatca aatattttta cttctaaatc atatcctttt
     2521 tcttttaaca aaggttttgc aacttccaat atctttgcat gtggatttga tgaagctcct
     2581 actagtattt ttttatcctc tttgtttgaa caacctactg ctgatattgc tattgctgac
     2641 accaatgcta ctgataataa tttctttaat ttcattttta ctccccctct ttaaaatgtt
     2701 tataattaat ttattttagt ttcttataag ccagatttcc tactccctgg attatctgaa
     2761 ctaatattat aagtgctatt acagtatata ccattactgc tgtatcaaat ctttgatatc
     2821 cataaataag tgctatatta cctaatcctc cagctcctac tgcacctgcc atagctgtat
     2881 aaccaagtat agatattatt gataatgtaa ttccagatac tattgaaggc atagcttcct
     2941 ttatcataac tttaaatact atttgtcttt ttgtagctcc aaaagatttt gctgcttcta
     3001 tcaatccttt atcaacttca tttaatgagc tttctataat tctagcgata aacggtgctg
     3061 ccccaatagt tattggcact attgcagcag tttcccctat acttgttcca acgattaatc
     3121 tagttattgg aatcattgca actataagta tcataaatgg gaaacttcta aatatattta
     3181 ctataaatcc taatgcacta tttattgtgc tatttggttt taatccatct ggttttgtaa
     3241 ctactaatat gattgctaat ataaatccta atattgttgc cactatagtt ggtactataa
     3301 ccatatacaa agtttgtaac aatgcatttg gaaatagtgt tgttaaaaag tcaattaaac
     3361 tattcattta tcatttcctc ccatcttatg cccttactag ttagatattg ttttacttgc
     3421 tctccacttt tatctgaaat gtttataata agtgaaccta aaatatcatc tttaaattgt
     3481 tctaatttcc caaatattat agacacatct atatttagtt ctcttgccat agtcgtaata
     3541 atagcttcat tagaaatatc tttagggaat agtatcttta tatttgttcc ttttggcaat
     3601 attatacttt cttcacctat taattttcta agtcctttag tatttcttaa gaatatttct
     3661 tctgtatctc ctacctctaa tacttctcca ttttccatta ttgctactct tccacaaatc
     3721 tgctttataa cttccatttg atgagttaca actattattg ttattcccaa ttttttattt
     3781 atatcttcaa gcaatgaaag aattgacttt gtagtatttg ggtctaatgc tgaagtcgct
     3841 tcatcacaaa gaagtacttg tggatttagt gctaaagctc tagcaattgc aaccctttgt
     3901 ttttgtccgc cacttagatt tctgggttta tcatttttct tttcagatat tccaactact
     3961 tctaataact ctaaaactct ttttttaatt tctgcctttg agtatccaaa acattctaat
     4021 ggcaatgcta cattatcaaa tacagttttt ctttcaagca atgaaaagtg ttgaaaaatc
     4081 attcctaact cttttcttaa atctctcatt tgcttttcat tcaatgactt tacttcttta
     4141 tcagaaacaa gtacactgcc ttcttggtat tcttctaatc cgtttataca tctcagtagt
     4201 gttgatttcc ctgcaccact atgacctatt attccaaaaa tttctccaga ttctatttca
     4261 atacttacat ctttaagcac ttgaatcttt ccataatact tattaacatt ttttatgcta
     4321 atcataaaat ttcctcccat atattctgct cagtttttat gtttatcctc tcttatgtat
     4381 tacaacaaaa taaaagcccg agtataaact caggcttaga aagctattta tactcatcta
     4441 tcaactatat tagttgcagg atttagcacc aatgtatata aaatatactg gttgccgggt
     4501 ttcaaagggc cagtccctcc accactcttg ataagacgtt atttaattta attctattat
     4561 catttgttgc tatgttatat atgatatatt cattaatagt atttgtcaat aaatttattt
     4621 aaatttttta ttttttatta tcgttaatta actattttta attagttttc tagctcctaa
     4681 cactttatat tttaatgtct atataactat agaacatcat tataagaaat aaattaaata
     4741 acatcaatct atctaaatca tctgtaaagt aaattaagaa aattaacatt ttatccaaac
     4801 tctatcctac tattaaataa aataatttaa atacaatgat gattatatag ctctagtact
     4861 ttagacttta tcaaatgttt aagttctata ggttctatta ttgtagcatt tttcccaaaa
     4921 gttaaaaaat attcagccac ccagttaagt gtagattttg gtatatacat atctatgtat
     4981 ccacctttag acaagatttt caaccctctt gccaacaaaa attctgtttc acaacgtttt
     5041 actccctcat ctgtcaaatt aattttaata tgataatctg tacctacttc catattctta
     5101 agataatcat gtatagaaga taaaacctta tatttcttac ttgataagtt ttctatactc
     5161 aaaatcttca caattctgtc tacacgaaat tccttaatag attctgattt tatacagtaa
     5221 gctggacaat accaaagtcc attcatggca tacaaaccaa taggaacaat ggtacgtaca
     5281 ctattttttt gctttgatga atattcaatt ttaattgtaa attcattcat tactatatta
     5341 aacaattctt tcaataatgg tgttgaacag tgcctatctg gtgtccaaaa aacaaattta
     5401 cgccttatat tctcaatatt atattgaata tcatttggta tacaatttaa aaattttttt
     5461 aaaacagaaa tagtttcttg ctcaaatggc aaatcattat aatattctag tgactgatat
     5521 gcaaagaata tagattttac ctcactttct gaaaatgcaa tcggtggaag tatacgctct
     5581 ttaagtacat gatatccacc agccgctcca acttttgaat ataatggaaa tccaatactc
     5641 tccagcacct gtaaatctct tagtatagta cgttttgata cagaaaattc ttctgctaat
     5701 tctcctgctg taaaacttct ttttgcattt attgttatca tcagttcaat taaacgctca
     5761 gctcttgaca tatttatact cctaatttaa atatagtgac aataattgtc actattatat
     5821 gataagatat tcttgcatct aaagcaataa taatagtaaa gaaagaaggg attgatactt
     5881 tatggaaaat gatactatta aggctgatga tattctcaat tattgtctat caaacttaga
     5941 tgatgttgta ctaatggata gttgggggga acgagcaatt tactacaatc ctaatggtgt
     6001 tttaaagcga ggggtatatg ttcttaccat taaggaaaaa gacagtaata atgataaagg
     6061 ttcgttagtt agtcgtccaa atgtataccg tgtgaatata ggattaaaaa aagaaacttt
     6121 tattgaaatg tttggatata ttccaaagcg tccaggtgta ggtcaaatag ttgatatgga
     6181 ttttgatttt acaaaattag acacaatcat gccacatcct atctactcat ggatgggatg
     6241 gatatgtgtc ctaagcccta ctgaaaagac atttgagaac tttaaaactt taataggaga
     6301 atcttacaat tttgcaaaac aaaaatttaa aaagagaaaa aacaagtaaa aacgtgtgaa
     6361 aataacctct aaataaatta taataaaaat attaaggtta caagataaaa attaatatct
     6421 taataacatg caaaaaagcg aagccaaaaa atgtattatt tataaggctt cgctatatct
     6481 caaataattt atttcaaatt tcaaaactat ttattctatt atttcttatt atttcctgtt
     6541 attcctaatc tatcattcat atcttttatc atttttctaa aaccactacc tatatcattt
     6601 ccaacatctt cagttccatg aactaatttt ataaagtcat tttgaccttt tttcatatct
     6661 tcatcagata ccttatctaa tttttctatt ctcttatcta tatcttttac taatctttcc
     6721 ccttcagcga ttaggtcatc attcattttc tttaactctg tatcttctgt tttaccatct
     6781 tttttaagag atgttacaaa tgattgtatt ttatctctac tatctttata tgcagccttt
     6841 acatcactta gatatttttc atttcctgga tattctttat ctttaaactg attatttata
     6901 ctttctggag tatcataaat agaatatcta tttagacctg ctatataatc attatagctt
     6961 gttgaataat aattcatata tccttcatta ttatttacag attcattagg attttttcct
     7021 gtattttcac ttggtttttc attattttta gtacatgcag ttatacctat agctaatact
     7081 agtgctaatc ctacaaataa agttcttttt aatttcattt ttattaccct cctagatgtt
     7141 ttatactata aaaaatttta ataattttct ttcttaattt agtatttcta tatttttata
     7201 ttttatttac taaaattaaa caagttataa tttatttttt tggaaatcat aatgaataaa
     7261 taattaaata aaaaaattat aattttatca ttgatattga ttatcattca tagtataatc
     7321 taattacttg atattaattt tcatttgaag gaggttatct tttatgtctt gttgtaattc
     7381 acaattttgt tgt
//
LOCUS       DAGSHT010000168         7376 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGSHT010000168 DAGSHT010000000
VERSION     DAGSHT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.174"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            382..1218
                     /locus_tag="MJB72_002945"
     CDS             382..1218
                     /locus_tag="MJB72_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8264020.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1571..3148
                     /locus_tag="MJB72_002946"
     CDS             1571..3148
                     /locus_tag="MJB72_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY8264021.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3141..4325
                     /locus_tag="MJB72_002947"
     CDS             3141..4325
                     /locus_tag="MJB72_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBY8264022.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4343..4942
                     /locus_tag="MJB72_002948"
     CDS             4343..4942
                     /locus_tag="MJB72_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY8264023.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4966..5436
                     /locus_tag="MJB72_002949"
     CDS             4966..5436
                     /locus_tag="MJB72_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8264024.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5795..6541
                     /locus_tag="MJB72_002950"
     CDS             5795..6541
                     /locus_tag="MJB72_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBY8264025.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6602..7276
                     /locus_tag="MJB72_002951"
     CDS             6602..7276
                     /locus_tag="MJB72_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBY8264026.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
BASE COUNT         2851 a          743 c         1341 g         2441 t
ORIGIN      
        1 ataaattaaa aattatatta ccaatgtata tatttgagaa aagattagta caaagctata
       61 atctgaattt caataaatat atatataatt aagttgaaac acttgaaaat actggaaaaa
      121 attgaaaata gtttttgtaa tataaaatgt cgattgattt taaataatta aatatacata
      181 agaaataatt aaatatattt caatgaaaat atgataaaat atgacaaatt aattgatgac
      241 aataaaaaat attttgttat tgtagtatta tagggtaaat tgcaaaatat atacgaaagt
      301 aaaattttaa atattgacat taaattaaat atactatact atgtaagtgt gatgagagaa
      361 tttttttaga ggggatttat agtgaatata aaaaagatat ttaataacaa tgtagtagta
      421 agctccctag aagatggcac agaaataatt gtcactggtg ctggagtagg atttaagaaa
      481 aaagtaggcg atttgataga tgaaaatttg atttctaaga aatattttgt tcaagacgat
      541 caaagggaca aatacaatca aatacttaat aagacctcta ttgaatactt taaaatttca
      601 gaagaaatta tagaaaaggc taatgaagtt ttaaatactc aagtgaatga ttcaataata
      661 ttagccttaa ctagtcacat agaatttgct gtccaaagag aaaaacaagg tataaaatta
      721 ccaaacttaa tattgaatga gacaaaacaa ctttataggg aagaatttga atttggatta
      781 tgggcaattg atgaaattga aaaaaggata ggaataaaat tgcctgaaga tgaagcagga
      841 tatattgcta ttcacataat caatggtttg gaaaattctc aaaaagatga aggtattaat
      901 atactagagt tttcaaaaca agttattcag ataattgaag aagtacatgg atttaaattg
      961 gatgtaaatt cattaaatta tactagactt ataactcact taaagttttt tgttcaaagg
     1021 attctccgaa aagaaactta caatgatagt gatattgaag atatgtataa gttagtgaat
     1081 aaaaattaca ataaacctaa agtgtgtact gaagaaattg cagcattggt attagacaga
     1141 tttgaatata agattagtaa agaagaagaa ttatatttaa tgattcacat aaataaaata
     1201 acaaacagtg gttgttagga ttgtaactga taaagcaggc aaaacctaag tcattgagct
     1261 taactattag attaagtttt ttgatttagg tttttttatt agatacaatc aaaatacaga
     1321 cagtgacttt taggattgta actggttaag caggcaaaac ctaaatcatt gaatttaact
     1381 attaggttaa gttttttgat ttaggttttt ttattagata caatcaaata cagacagtgg
     1441 ttgttaggat tgtaactggt tgagcaggca aaacctaaat cattaaactt aattattaaa
     1501 ttaagttttt tgatttaggt ttttctataa atttgattat gtttttagat aaaaatagga
     1561 ggggaaaatt atgaaaaaaa ataagttagg tttatggaac ttcttccaaa tgttaggaaa
     1621 aacgtttatg ttcccaatag cactacttag tgtttctggg atgatgctag gacttggggc
     1681 aggttttaca gaccctaaga tgattgaaat ggttccattt ttagggaatg aatatgttaa
     1741 cttagtttta aacttcatgt taacaatagg attatttgca tttaacaact tgggagcatt
     1801 atttgcaatg gcaattccac taggattatt aaaaaaagaa aaagagtttg gtgctttttc
     1861 aggattagta ggatttatag caatgcatat aggtacaaat ttttatttaa caagagctga
     1921 tttgttagta ccagcagaac agatgtcaac taatggtcaa gctatgatta tgggtataca
     1981 aacatataat acaagtgttt taggtggtat tgtagcagga ttaattgttt atgcaatata
     2041 tgataaagtt agtaatttaa agataccaga atcattagga ttctacagtg gaccaagatt
     2101 agttccaatt gttagtttaa tagttatgag tatagttgga ttattaatac ctattgtttg
     2161 gccaccattt ttcaatgcat tccaaagttt aggaagatgg atttcttcag caggaccatt
     2221 aggatatttt tcatatgcag tggcagaaag agttacaatt ccatttggac taaatcatct
     2281 tgtaacatct gtattccgtt ttacaccaat aggtggaact gctgttattg atggagaaac
     2341 gtactttggt acattgaaca tgtttatggc atatgtagaa aataatcaga ttatacctct
     2401 tgatttagct ggtaaaatgg aacaaggtaa attgatgatt caatatggat tagctggtgc
     2461 tgcattggct atctaccgta cagcaaaacc agaaaataga aaggcactta aaggactttt
     2521 aatatcagga gtgttgacag ttattatagg tggtataagt gaaccaatag agtttctatt
     2581 cttattcata tcaccagcat tatttgcttt ccatacattt atgaatggtc tagcaaacat
     2641 ggtacttcca tacttaggcg tgttgatggg attcacagga gatttaattg catttataag
     2701 ttttggtgtg ttaagaggaa cagctacagg atggccaata gcagtattag tagcagtttt
     2761 atattttgca atatactatt ttgtatttaa gtgggcaatt attaagttta atataaagac
     2821 cccaggacgt gaggataaag aaataaaagt atctgaagga aatggttcaa atttcaaaaa
     2881 tttgtctaca tataaaggac aacaaatgat atctgcttta ggtggtcaat ctaatatatt
     2941 atcactagat aattgtgtaa ctagattacg tttaaaatta caagatgtat cattaataaa
     3001 tgaggatgca attaaagagg ctggtggtat agcagttgtt aaattggatg agcatacaat
     3061 acaagtaatt ataggaaccc aagtgtattc tttaagaaag cagatggata aagctatgga
     3121 aggtcatttg gaggcatgta atgattaatt ttgataaaaa agtagaccga ttgggtacat
     3181 attgtaccca gtgggactat gtagaagata gatttggtaa gaaggatttg cttcctttta
     3241 caatttcaga tatggattta gagtttccat ttgaaataac agaggtgttg caggaaaggt
     3301 taaatcatag agttttggga tacagtagat ggaaacatga tgattttaga aattcagtta
     3361 cgaattggta tattaaaaga tttaattgta gcgttgatag taactggata tattatagtc
     3421 caagtgtaat gtattcaatt agcaaactta ttgagatgtt aagtaatgaa ggagatggaa
     3481 tccttataaa tactcctgca tacaatgctt tttatgatgt gataataaat aacaatcgta
     3541 atattataaa gtcacatctt gtaaatagga atggattcta ttcaattgat tttaatgatt
     3601 ttgagaaaaa gtgtaaaaaa tctaagatat ttattttatg taatcctcac aatccaactg
     3661 gtagagtatg gagtgagtta gaacttgtta agatgatagg aatttgtaag aataatgatg
     3721 tcaagataat atcagatgat atacacatgg atattgtata tgacaataga atgacaccta
     3781 tcttaaatgt tgcaaaggac tatttagatt ctgtattcat ttgtacatca gcgtctaaat
     3841 ctttcaatat accagcattg actgggtcat atgtgattat accaaatgaa ggtattagaa
     3901 ataaatttga aaatatcact agataccgtg attttgtaaa ttcgccagca attctagcaa
     3961 tattagccac aatggtctgt tatgatgact gcgaatattg gttagatgaa ctattaaaat
     4021 accttaaaga gaatcttaaa tatacgattg attatataaa agataattta gaaccattga
     4081 aattggtcat gccagaaggc tgttactttg cttggataga tttttccgaa cttaaaataa
     4141 gtggtgagaa attccaaaaa ttattgatgg atattggaga agttgctatt atgtcggggt
     4201 ctgtatatgg tgaagaaggt gagttttatt taaggttaaa tgtggcatgt tcaagagaaa
     4261 aacttgaaga tggtttaaat aggattaaaa aaactatgga ttatattgaa aaaagaattt
     4321 tatagtagga agggagatag ttatgggata tttaaaaaca atggttgatg ttatgactac
     4381 tgaaatgaat gccataagat gtttaataga tgaagcagga atagaatatg aaagtatagt
     4441 tagtgaaatt gcaaattgtg aaggcaaagt aatttttatg ggtgtaggaa aatctgcaca
     4501 tataggtaag aaattggcag ctacatttgc tagtactggt acacctagct tttttgtaca
     4561 tgcaacagag gcagtgcatg gagatttagg aatgattgaa agtaaggata ttacaatttt
     4621 aatatcaaat agtggaaatt ctatggaagt ggtaaactgt ataaaatata ttaaagcaat
     4681 aggttctaag acaattgcat ttacttcaaa cagaaattca gtactggcaa aagagtgtga
     4741 ttatgcttta atatatcctg caaaggatga agcagaccat ttaaatctag caccaacaac
     4801 atcatctaca attactttgg ttttaggaga ttctattgca tgtgcattat ctaagagtag
     4861 taattttggt tcttcagatt tttataagta ccatccagga ggaagtttag gtgagaagtt
     4921 aaaaacagct aacaatggat aaaagcaaaa aggagagtag tttttatgtt caaaatgttt
     4981 aaaaaaaaat caataaaatc acccatatca ggaaaagtaa tagagttatc taaaatacaa
     5041 gatggtgtat tttctcaaaa aattatggga gagggtgtag ctatagattc tacaggagac
     5101 attgtatatg ctccaatcaa tggaaagata gtagtagtag cagagactaa acatgcattt
     5161 gtaatagaat tagaaaatgg gatggaactt cttattcatg ttggtttaga tactgtaaat
     5221 ttaaaaggcg aaggttttga ggtattggtg agtatgggag aacaagtgaa ggaaggaaca
     5281 ccaatgttga aaattaaccg tcctttgatt gaatcaaagg gtatatcact tattactcca
     5341 gttacaataa ctaatcattc agagtacaat atgaatacat gtaatgtagg caatagtgtt
     5401 gaaggtggga aagatactgt aattgagttt aaataaagaa ctaaaataca aggttaatat
     5461 ttataaatac ctataaactt atgttttata agattgattg tatagatttg ttgtataaaa
     5521 aagaactctt taattataaa ttaaggagtt cttttttatt ttgtaaatat tgtttagatt
     5581 tatatagaaa aacacttagt aataaataga ataaataagt atttatatag ctaaaaatct
     5641 aaatagtttt gtttaaatat atttttatat tttgtaattt caaattaata aataattaga
     5701 tttgataaac aaaaaataaa aatgttgatt acattaataa actttaatat aatatattta
     5761 tgaaaaatgt aaactgtgaa atggaggtag taacatgaaa attgaaaatc tttctattga
     5821 tgatattaaa cgaggctata tatttaatga tgatttaaat tgttatagtt gcattaaatg
     5881 tacaaaaaca tatgaggtag gagaaatata taaaataggt aatagattct ttgaagcttc
     5941 aaaagctatt gaacttcata tggaattaga acatagtgat tatttagagc aattgcttaa
     6001 tagtgattca aagtacaaca cattgactga aaatcaaaaa caattatttc aattatttat
     6061 tagtgattta ccagataagg agatagcaaa acaggttgga gtatccacat ctactatacg
     6121 ccatcaaaaa tttattttca gagaaaaagc aaaacaggct aagttctatt tagcactata
     6181 tgaatctgta tttgaagata aatctaataa aagtagtact ataataccaa tccatgaaca
     6241 tgctcgaatg gtagatgaaa gatatgtagt aactgaagaa gagagaaaac acattttgga
     6301 tacatcattt gaaagtatga atcctttaaa attaaaagta ttttcttcaa aagaaaagaa
     6361 aaaggtcgta attttatcta agattttgga acaatttgaa cgtgggaaga aatattcaga
     6421 aaaggaagtt aatcagattt tagaatctat atatgatgat tttgcaacaa ttagaagata
     6481 tctaattgag tatggtttta tgagtagaaa taaagaatgt acaaaatatt ggttaacata
     6541 attaaaataa taaattttac taatgtgtaa tgttattttt atctaggggg aggagtcaaa
     6601 tatgaatgat gaattaaaat ctattttata caataaaggt gtagacattg ttcgctttgt
     6661 tgatatatct gaatttccaa taaatcaaac acaaggtttt tctaaagcaa ttttattttg
     6721 tatagggtta tcaaaaaaat ttattacaga tatatacaat aacttaccta cagatagtga
     6781 tgagtttttg gaaaaagagg agaaagtaga agaattagct gactggattt caaaatatat
     6841 tcaaaataaa ggatatagag cgtattctca atcggagaaa aacaatctag aacatggata
     6901 ttttgaaaaa gcctatatta atccagaaat gcaatcaggg attagtcctt taccacataa
     6961 gacaatagca aatattagtg gcattggatt tatgggaaaa aataatttgt ttgtaacaga
     7021 ggaatatgga tgtgctttta gcatgtgtac agtacttaca gatgctccaa tttctgttga
     7081 aagatatcct ttgattgatt caaaatgtat ggattgcaat gtttgtgtag agaactgtcc
     7141 tgcaaaagct atacatggaa atgaatggac tttacctgga aaaagagaat ctattattga
     7201 tgtttcaaag tgtttttgtg tattgaggtg tatgatgagt tgcccatggt cattaagata
     7261 tgcaaatcaa aaataacttt actagagaag gtattaaatt gaatattata aaatttttaa
     7321 aatgtaagat tatataagat ttttcaaaat aaaaatttgt atagaacact cagaaa
//
LOCUS       DAGSHT010000169         7289 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.175, whole genome shotgun sequence.
ACCESSION   DAGSHT010000169 DAGSHT010000000
VERSION     DAGSHT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.175"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            16..1098
                     /locus_tag="MJB72_002952"
     CDS             16..1098
                     /locus_tag="MJB72_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY8264027.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            1247..1408
                     /locus_tag="MJB72_002953"
     CDS             1247..1408
                     /locus_tag="MJB72_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBY8264028.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEITQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            1749..3017
                     /locus_tag="MJB72_002954"
     CDS             1749..3017
                     /locus_tag="MJB72_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBY8264029.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            3212..4000
                     /locus_tag="MJB72_002955"
     CDS             3212..4000
                     /locus_tag="MJB72_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY8264030.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            4000..4449
                     /locus_tag="MJB72_002956"
     CDS             4000..4449
                     /locus_tag="MJB72_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBY8264031.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            4453..6717
                     /locus_tag="MJB72_002957"
     CDS             4453..6717
                     /locus_tag="MJB72_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY8264032.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMSLFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            6857..7168
                     /locus_tag="MJB72_002958"
     CDS             6857..7168
                     /locus_tag="MJB72_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264033.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         2828 a          752 c         1314 g         2395 t
ORIGIN      
        1 tttaggagaa aataaatgag aaaattatcc aaaagagaaa aagattttat tcaatatgga
       61 tttttattat ttttgatatg tattactact tatttagtat taactacttt agatataaaa
      121 ctaataccta aaattataag gctagtaaat aagacttata tatttcttgg catattgaca
      181 attatagtat atatgataat tgaatctatt gtaactcatt tgataataaa tgcagttcaa
      241 aaaacaaaag taaaatttat tggtattaaa atggctacaa tgggatttta ctataactta
      301 gttactccat ttgcatctgg gagtcaacca atgcaaatat atgcacttac aaaatatgat
      361 gtaagcctta gtaaatcagt ggctatagtt actaataaaa ctgtagtatt tcaaagtaca
      421 gttactgtat tttgtggaat tttaattttg ctaaataagt cattactgat ggagcaagta
      481 cattctgtga aagtcttgat aacttttgga atgattatga atgtaagtat gttgttaggt
      541 ggactactaa tagtatttag tccaaaacaa gtaaaaatga tagcagaagt tttgattaat
      601 tttttatcta aatttaaaac atttaaattt ttagattcaa aacgtgatag aataaatcat
      661 tatattgatg attataatta ttctataaaa atatttatta aaaatgtaag aatcttagtt
      721 ttaagtatat tacttactgt gttacaatta atcgcatatt tcagtatagt ttattgtgtg
      781 tataaagcct caaatttaag aggagtttca tatgtacata taatgacatt acaggtcttt
      841 ttatatatgg ctatatcacc aattccaaca ccaggaaatg ttggagctaa tgaggttaca
      901 ttttttacta tgttctccaa tataattcca aaagaattat tggggtactc tgtactctta
      961 tatagtgggt ttgtatacta ttttatatta atagtttcag gtttatttac atttggaacg
     1021 cattatacat taaattcttg gaaaaaggaa gataaagtaa attctaattt agataaggtt
     1081 attgaaaaat taaattaata aaataattag ggaaattcta tatataaata tggtgttgaa
     1141 taagctatat tatattgaga tatttccctt ttttgattaa aattatagaa atttgaaata
     1201 ataagtaata aacagaaaaa gtgatattgt tttgaaagga gaaactatga aacaagataa
     1261 acaaaatgaa aaacaaagtg agatttctaa gataaaacaa agcaatgata ataaacttga
     1321 aataactcaa gaattacatg taagtaaaaa tatagggaat atgcttggat atagaataga
     1381 taagaaaaaa atgaatagac caagttaatg aaaatgttga aaatggtaca ttttaaaaaa
     1441 tatatataat gaaatgtttt attgttaaat ttaaacttat tttaatggag gaaaaagtcg
     1501 aaaaatatct aaaaagatat ggatataaaa aatattaata tatatatact ataaacttga
     1561 caaaatcaac cttttaataa ctagcaaaaa atagcaaaat tatataagcg ttatttaatt
     1621 gtcaaaaatg aaagctaaat tgcatgtcct aaaaaaaatg attgataaca tttatttgtg
     1681 tgctataatt atatagtaaa acaataagta tattgcataa tgtatgcaaa tcgggaggag
     1741 agacggacat ggaagcatct atacacacaa ttgccagcaa taatttattg ataatttttg
     1801 ctattgtaag tataactgga ataatttgta gtaagcttag tgaaataata aaggttccag
     1861 atgtagtgtt atatttactt gtaggtattt taataggacc atcattttta aaatttatag
     1921 atattcgtgg atttcaaata gaaaatcaac taatacttac atttggttct gcatttattt
     1981 tatatttagg tggaaaagaa ataagtttaa aagtattgag aaatgtgaag atatctgtat
     2041 tcttattagc tactttagga gttgtgatat ctgcttttat aatgcaacaa gtaataggat
     2101 ttacttttgg gataagtgca atgactgctt tactagcagg aactataata gcatcaacag
     2161 acccagccac tcttgtacct atatttaatc aggtaaagat aaaggatagg gtaaaacaaa
     2221 ctgtaataag tgaatcagct tttaatgatg caacaggagc tatactaaca tcagcagtgt
     2281 tggctgtaat tttgtcaaat aaattttcat taggtgaaaa tatttatgaa ctggggacaa
     2341 tggttattgt cggagtttta gttggtttaa ttacaggaat actgcttttg aaattagtta
     2401 atgataaacc ttatggaata tttaaagatt ttgcaccaat aatatctgtt atttcagttg
     2461 tagttgcata tgaattatct acaaaattag gtggaagtgg atatatgtct tgctttattg
     2521 taggtataat aactggtaac aagaaaaact ttaaaatttg gttaagtcaa aaatcttatg
     2581 atgcagattt ttatgttgct gaaactttag gtactatttg tcgtatggca atatttataa
     2641 tattgggtag ccaagttaat ttggctcagt taagtaaata ttttatgcca tctgtaatta
     2701 cagtattagt attaatgttt atagcaagac cactttgcgt tttagtttgt acacttgtag
     2761 acagggaagc aaaatggagt aaaagtgaaa tattatttat gatgtgggta agggaaacag
     2821 gcgtaatacc agcagcactt tgtggtatta tttcagcaat gaaagttcct ggatacgaag
     2881 tgatttcatc tgtagtattc atgactatac taattacatt agtaatacaa ggaagtacta
     2941 caaaattagt agctaaaaaa cttggattat tagaagaaga agtgataaat ataagtgaaa
     3001 aagtatcaac tttttaaagt tttgctttaa aatagtacct gttaatggct attttaatat
     3061 tgtctaatga tgattattga aatagtcatt tttttatgta taagtaattt acagaattta
     3121 catattagac atatttatta tcaaaatttg ttgaagctat gatataatta gttgtattta
     3181 aaataagaat agcaaataag ggaggaaatt tatgtttacg gttatggata ttgtacagcc
     3241 agatacagta gaagaagcgt actcaatttt aaataaaaga aaaactaacc aagtaattgg
     3301 tggaagtgct tttttaagaa tggggaaaaa aagaatagga acaggaatag aattatctaa
     3361 attaaattta gattatataa aagagtatga agattacgtt gagattggtt ctatgactac
     3421 ttttagaaca ttagaaacaa gttccataat aaaaaataat tttggaagaa taatagaaga
     3481 ttcagttaaa aatataatag gagttcagtt tagaaatgta gtaacagttg gtgcaacagt
     3541 attttctaaa tatggttttt ctgacttgat agtagcatta ctttcattag atacagaagt
     3601 agaattatat aatattggga gaattagtct tgaagagttt ctaaatagag attatgagaa
     3661 agatttactt attaagatat acataaagaa gacaaataag aatgcttctt ataaatcact
     3721 tagaaatgca aagagtgatt atccaattct aaatgtatct gtatcaaaac atatggaaca
     3781 atttaagtta tgtgtaggag caagaccgca gaaagctacg attgcaaaac aagctagtga
     3841 atttctatct aacaatgaaa tcaatgaaat taatatagat aaagcagtag aaatagcatc
     3901 agaagaacta acttttggtt ctaacatgag agcatctagg gagtatagaa aagctatgag
     3961 taaagtactt ttaaaaagag ctataatgga ggttatttaa tgttagtaaa gattaatata
     4021 aatgggaaga aaaaaagtgt tgatatagaa ccagaagaat atctggttga tactttaaga
     4081 aaagttggta atttaagtgt gaaaagaggt tgtgacacag gctgttgtgg tttatgtaca
     4141 gttttaattg acaagaaacc tacattatcc tgtgcaacac tgacattgag agctttagat
     4201 aaagaaataa ctacaattga aggtttagaa aatgaagtta aagaatttag tgaagttttg
     4261 gtaaaagaag gagctgagca atgtggtttc tgttctcctg gctttatact tactgtactt
     4321 gcaatgaagg atgagcttga aaatcctaca gatgatgaaa taaagcatta tcttacagga
     4381 aatctatgta gatgtacagg gtatatggga cagttaagag ctatcaaaac ttatctggag
     4441 gtaaaataga acatgaatat agttggaaaa ggaatcaaga aaatagatgg aatgagtatc
     4501 acaacaggaa agcctgtata tacagatgat ttatcagcaa aagatgcttt ggtggtaaag
     4561 atacttagaa gtcctcacgc ttatgcaaaa ataaaagata tagatacatc aaaggcagaa
     4621 atggttgatg gtgttgaatg tgttttaaca tataaagatg taccaaaatc tcgatttaca
     4681 cttgctggtc aaacatatcc agagccatct ccatatgata gattaattct tgaagatgtt
     4741 gtaagatatg taggtgacga agtagctata gtggcagcta tagatgaaaa aactgcaatt
     4801 aaagcaatga atcttatcaa agtaaagtat gaagtactta cacctgtttt ggattttgaa
     4861 gaggcaatag gaaataaaac tatggtacat gatgaagata gacattgtaa ctttgatata
     4921 ggtatgcaaa gagaaaaaaa tatagtatcc catcatgatt atacaaaagg tgatgtagaa
     4981 aaagtattaa gtaattgtga tgtagtgatt gaagaaactt attatactca agctcaagca
     5041 catgcaatga tggagacgta tagggcatac aattatttag accatatggg aagattggtt
     5101 gtagtaagtt caactcaaat accatttcat gtaaaaagac atttatctcg tgcactagaa
     5161 ataccagcta gcaaaattag ggttataaaa cctagaattg gtggcggatt tggtggtaaa
     5221 caaactgcat gtgttgagat tttttctgca attgttactt taaaaacagg gaaaccagct
     5281 aaaataattt atgatagaaa agaaactttt aattgttcaa caagtagaca tgcaatgaga
     5341 ttaaaagtta gactaggtgc tacaaatgat ggcataataa aggcaataga tataaaagca
     5401 ctttctgata caggagcata tggagagcat gcttctacaa ctattggttt ggttggagaa
     5461 aaaactttac caatgtataa taaagttgaa gcaacaagat ttattgcaga tgtggtatat
     5521 actaataaga tgccagcagg agcacttaga gggtatggag catcacaagg gtgttttgct
     5581 gttgaatcaa ctataaatat gcttgctaat aaattaaaaa tggacccaac agatttaagg
     5641 ttaaaaaatg tggtaacaga aggtgaaact acatttgcat atggtaaaac tttaaatagt
     5701 gtcgatttaa taaaatgtat aaataaaggc aaagaactta ttggatggaa tgaaaaatat
     5761 ccttgtaaac atcttgataa tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa
     5821 ggttctggta tacaaggtct tgataagtct tcagctgaaa taaaattgaa tgactgtgga
     5881 gattatactc ttatgatagg ttctactgat atgggtacag gaagtgatac gattttagct
     5941 caaatggctg ctgaggttct tgatacaagc attgaaaata taacagttat atcagctgat
     6001 actgacttag ttccatatga cccaggttca tatgcttcaa gtactactta tgtaacaggt
     6061 atggcagtag ttaaggcagc tcaagactta agagaaaaaa taatttctgc tggagcaact
     6121 aaactaggtg ttgagaagga tgaagtagaa tttgatggta taaaattaac tagtaaaaaa
     6181 ggtagcactt tattattcga tatagcagtt gattctacaa ttggtccaga tactaatcaa
     6241 ttaataggtc ttgcttctca tggtagccca gtatctcctc caccttttat agcaggtttt
     6301 gcagaaactg aaattgacac agaaactgga aaagttgatt tagtaaatta tgtagctgta
     6361 gtagattgtg gaacggttat aaataaaaat ctagctaaga tacaagttga aggtggaatt
     6421 gttcaaggga ttggaatgtc attatttgaa gaagttagat acacagaaaa aggaagcatg
     6481 gacacaaata catttatgca atacaagatt ccagctagat gtgatattgg aaatgttaaa
     6541 gtagattttg ttgaaactta tgaaccaact gggccatttg gtgctaaatc tgttggagaa
     6601 gttgttataa atacaccttt accagcaata caagaagctg tgtttaatgg aattggtgta
     6661 agaataaata cactccctat tacacctgaa aaagttttta tggaaatgaa taaataatta
     6721 tttatgaaaa tttagactag ttttacatgt taaacaacca gttatgtcat acctgttgaa
     6781 agatatttaa taagtagtta ttataatatt atgaatttta cttataaaat acaaaattaa
     6841 ataaggggat gatgttttgg ctggtaatct aaataatatg agagcagtaa ataattttag
     6901 aggagataag aacattttag aatgtttagt cagctttgag ggtcgttcaa taagtcagag
     6961 aaaagtaagg gtatttttta aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga
     7021 ggaaatttct aaattggttg aaaatgttgt tttaaataca tcatatcaag aaatgttata
     7081 tgatgaaata gagaaacaac tggaaattga ttgtataggt acttggatga tattatctaa
     7141 attaaaagat ggtagtagag ttcactgatt ttaaaataaa ttattagagc ctaattttaa
     7201 ggctcttttt tgctgttttt aattaacaat agaaaattta tctttagttt gtatataatt
     7261 ttaatatcat atattctata tatataaaa
//
LOCUS       DAGSHT010000170         7271 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.176, whole genome shotgun sequence.
ACCESSION   DAGSHT010000170 DAGSHT010000000
VERSION     DAGSHT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.176"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            20..403
                     /locus_tag="MJB72_002959"
     CDS             20..403
                     /locus_tag="MJB72_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264034.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
     gene            911..1294
                     /locus_tag="MJB72_002960"
     CDS             911..1294
                     /locus_tag="MJB72_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY8264035.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1296..1646
                     /locus_tag="MJB72_002961"
     CDS             1296..1646
                     /locus_tag="MJB72_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264036.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1747..2205)
                     /locus_tag="MJB72_002962"
     CDS             complement(1747..2205)
                     /locus_tag="MJB72_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264037.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2250..3038)
                     /locus_tag="MJB72_002963"
     CDS             complement(2250..3038)
                     /locus_tag="MJB72_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8264038.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTKLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3177..3614)
                     /locus_tag="MJB72_002964"
     CDS             complement(3177..3614)
                     /locus_tag="MJB72_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBY8264039.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4436..4945
                     /locus_tag="MJB72_002965"
     CDS             4436..4945
                     /locus_tag="MJB72_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264040.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIGFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            5350..5886
                     /locus_tag="MJB72_002966"
     CDS             5350..5886
                     /locus_tag="MJB72_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBY8264041.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(6320..7120)
                     /locus_tag="MJB72_002967"
     CDS             complement(6320..7120)
                     /locus_tag="MJB72_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264042.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
BASE COUNT         2659 a         1036 c          987 g         2589 t
ORIGIN      
        1 agaaaggaat gtgctttata tgtctacaga caaagttgtt ggaatcatag atgaagaaac
       61 ggcagaactt gcaggaattg aatatactgg taaaatttat gcttcttctg gtgttataaa
      121 acatataaag aaaaaacata gatgtcaact gagtaaggat atttttaatg atataataga
      181 cactataaaa atggtattaa agtcacctga atatatagga agtcatccta aaaaacctgg
      241 aaaaagtgtg gagtttataa aaaaaattga tgattatatt ttagtagcta cagaattaga
      301 tacaaaaaaa ggatatttat atgtatcttc aatgtatgct ataaaagaag caaaattaga
      361 aagtagagta aacagtggaa gagtaaaaca atataatggt taacataata ttattaattt
      421 tccctaaaat attgaaaaat attattatat aagttataat attaatatac acgtagatat
      481 ttattagtta gatttgaggt tggaaaaggc acccaatacg ctactataga gtagttaata
      541 gagatgcagg atacgccgcc ctgccaaatc taattaaatt aaaaagagag gattaattcc
      601 tctcttattg tttttttgag atactaattt aatctataat agaaagattg aaggtcgttt
      661 atacaaaaaa attttcagtt atatacatta aactaattaa ataagatatt tataatctat
      721 atgtgataca tattatatat taataaaaga aaaatatata gaaaatcctt ataaaaattt
      781 aaattgtaat gaataggaag taattaaaaa gaatttttat tgatttttaa aaatttagtt
      841 agaaactata tcattatggt aaaaaatgta ttataatgtt tagaaatatt ataataagga
      901 gcgattaaac atgaatctat cgaagaaaat agaagataaa agaaaagcta aacagagagc
      961 tgaaaaagtt aagaaagcta aaattgctac agctggtgtg gtattaggag ctgttactgg
     1021 tgcagtgagt ggagtacttt tggctcctaa atctggaaaa gaaacaagag aggatataaa
     1081 agatgcttca caacaaattg ctgaaaagat aaatatgaaa actgtagatg taaaaggcaa
     1141 agtatctgag aagctagagg ataaaaaagg aaactttata gaatcaaaga aaaaaataaa
     1201 aaaatatata gataacaaaa agagtgttga aaattctagt gaggaagata aaataataga
     1261 tatagttgaa ccagttagtg ttactgagga ataatatgga caatacaata acgattaaca
     1321 taagtataat atatgtatta ttagctatac ttgcttgtat tgctttagtt tatttaatta
     1381 ttgtacttgt taaactacat aagattttaa ataatgttgg aactatgtta aatgaaaata
     1441 ctcaaaatat aaatacaact ttaaataatc ttcctaatgt tgtagaaact tttggagaag
     1501 taggagaaaa tatgaaagat gtaacagaag tagttactga agttacagca gattttcttg
     1561 ttactaaaga cagtgtcaaa tcaaatatgg atgttatagt tgatatattg actataatta
     1621 aaaacatttt tctagataaa aaataataag tttaattaaa ataagcctat aagatataat
     1681 ttaaacgacc ttaaaatagc atttataagg tcgtttttta ttagcttgat ttgacactat
     1741 gtgtttttaa taattggtaa tattcctctt tatcatttga acatatatat ctatgcttgt
     1801 tgtttcaatt acatccataa gaacatcttt acattcaagc tcaaaatctt ccattccttc
     1861 taaatattca tcatcttcat caaaaaagat ataagaatta tatagccatt cttcaaattt
     1921 ttcttcaagt atatcccaat acgaactcca aaataatcca caacctgata tataagatgc
     1981 tctactttga tttatataaa cctcctctag aagcttttgt aatctactgc tataaagaaa
     2041 atctgctctt ccactgcttg atattaattc atttaaatct tctattgaca ttaattcaac
     2101 tttaccactt ttatcataag gagtataatt ttcatcaacc catttatgtg gtttgttttc
     2161 tctgcatact gttgctatca actctacaac ttttgttctt atcatttttt tatcctaact
     2221 tatttatttt ttataaaata atcatgattt tataatttat ctatttcttg taatgctaat
     2281 ttaacagttt catatctatc agcaccattt attactacat caccttttac aagtttatta
     2341 gcttctccac ctacaacata aaccttttta cctaaacctt gcttataact agacgcctca
     2401 attattaaag aatctttcaa ttgccaatgt aaaatctgag cagccacttt atcagcatca
     2461 cctgtataaa cgattacatt ttcatacact ttattttccc ctcccccttg agaatttgat
     2521 atagatttgt ttagtatacc ttctgctatc aatttagcaa ctttatcttt attattaaca
     2581 taataatctg tatctgcttt actatctaca aaacatactt ctattaatat tgctggtgca
     2641 atagtatgat taagccaata aagacctcta acatcagact ttgcgcccct atctttgaat
     2701 actgtagcta acttagtatt cactctttct gcataggttt tgccattatt agtcttatat
     2761 attgtttctg tccctactgg agttgaagtt gtggcatttg aattaaaatg aatctgaaca
     2821 gccaaatcag tttcctgttt atttgctata gcacattggt ctgctaaatg attacttgat
     2881 tcatcaactc ttcctgtata aacagtagct ccacctattt taagccattt aacaattaag
     2941 tcattcagta ttcttgtttc tttgctttcg tttatatacc caacagctcc tgtacctttt
     3001 ccaattaaag tgtgccctgg tattattact actttcaaac taacatctcc tttacaatta
     3061 aatttaactt ctatttaata tatgcataac ctagaatttt gtttcttttt atatcttata
     3121 cttttattat aacccatttt gtaaaaatat agaatattaa gtaaataatg ttaaaattat
     3181 aattttgtat ataagacatt acttgattgc aataatttta tagcatattc atctattcta
     3241 tatccagttc tataatatat atgtttaatt ccagcttgaa ttatagcctt agtacaattt
     3301 aagcaaggaa agtgagttac ataaatagag caatttttaa ctgaaatacc ttcttttgca
     3361 caataataaa gggcattttg ttcagcgtga atggttctaa tacaatgtcc atctctcatt
     3421 tcatgaccaa cttcactaca atgcttatct ccagaaatac taccattata tccagtagac
     3481 actattctgt tttctgaatt gacaattata gctcctacat aagctctatc acaagttcct
     3541 ctttcagcta ctgtttcgca cagtctcata aaatattctt gccatgagca tctattttca
     3601 agttttttat tcaaattata tacctccaaa acttatatct aatatataag atttatacct
     3661 taaaaataat attaaaaaca aatttatacg ataaataaaa tttattaata ctgtttttag
     3721 tctaaattat ttcattttaa attaaagtta caaggaatct aaaattatat atatctttct
     3781 tcgtaatatt aatgttacaa tataaaaaac tgtctcaata aaatagagac agttttacag
     3841 tcaaagttta taatacaagt tcatattttt ctttaaattc ttttacagct tcgggccatg
     3901 ccttttgttc tgtaatacct ttttctttag ctattttaat tgcaatctct cttatcattg
     3961 cagcgccttc taatattccc atttttaatc tcccttcaaa tatctttttg tatagaatat
     4021 aaatagtgct aagatttatt acatgctgca tatacaatat ctatacaaca cataacttgt
     4081 aaattccttt tattttcata cgtctttaac cgacatataa ttatattcaa taagttttaa
     4141 aattatgaac tcaattataa ttatttttat ttttttagaa tctaaacact aaaaattgta
     4201 ggtaaatttg tcttatgcct ttataaatat ttctacagtt ttaattactg gacatatcta
     4261 tttttattat aaaaagcgct taaaaatcga gaatatccgc ataaaaactg tatataagca
     4321 aaacggttaa aggaaaaaga agaaatttac ttgaatgtta aaatgcgttg cttgaagcaa
     4381 tgggcagttt tgcctataat atcggtaaca actgaaagaa aggtggttag ccctaatgtt
     4441 aagcgaaaac attaaaacaa tcaggaagtc aaaaggactt tcacaacaag aacttgctat
     4501 aaaactgaat gtagtgcgac aaacaatatc taaatgggaa caaggactgt cagttcccga
     4561 ttctgatatg ttgatttcca tatcagaggt gcttgaaacg cccgtaagca ctttacttgg
     4621 agaaactatt gttgagtcgg aagttgatag cttaaaagtg atttctgaaa aattagagat
     4681 tataaacttg caacttgcac aaagaaaaaa catgagaaga aaaatgtttc attggttatt
     4741 tattttatta tgtgcagtca tattaatagg ttttgtggtc ttagggggac taaatagtcc
     4801 ttacttggat tggaattata ccgaccctga aaccgctgtt tttggagtag cttttcatgc
     4861 ttttgagtgg ctatttgtca gattagtacc gattgtcctt ataggagcaa ttattggaat
     4921 ctttttgaca cgtaagaaaa tatgaagtaa ctctgctttt tataatacag caagcgttcc
     4981 ttaaaagcgt cattctacat ggcaatcgag gacgactatg taaggaagaa cctttttgat
     5041 ttccaactaa gcgaagttat tgaggacgac agtaggccca aaaccgcatt aagtaaagta
     5101 caggaagcaa gactgctgga cttatactct catgcgtaag atgcaggaaa tgcgaggtct
     5161 ggtagcatga tttacaacga tttgtacaac gactacgagt agaaatatat ggagataagt
     5221 attatctacg aaatcagaaa agaaatgact tgataattta ctcatgttat aattattgat
     5281 gtgaaattaa taaatcgaaa ttcctttttt ggatttaaca atactgagta aggaaaaagt
     5341 gaatgacttt tgcaaggagg tgttaatatc aggcaagtaa agtcttttgt ttttccagtt
     5401 atatccttaa tattcttaga ccaaattagc aaagttctta taggattatt cttaatggac
     5461 tttgaaattg atataattgg gaaattttta agattcaatc ctgttcaaaa tacaaatctc
     5521 tcttatgggg gaaactttat tggtattcta tctaatttat gggtattggt attgtttaac
     5581 attttagtta tattagttat tatatctgga tatgcttttt ataaatcaaa aaatgaacaa
     5641 acaagctatt cagtaaaagt aattatgtct tgtggacttg ctggtacaat atgtagcttg
     5701 atagataaat tattttgggg aggaagttta gattttttgc agataccaag cttttttatt
     5761 ttcgacttaa aagactgcta ccttactgtt gcagaaataa tatttgttgt cataggaatt
     5821 ttgcataata gagaaatatc aatgaaagaa tacatatatt tttgctatcg tcagtttaaa
     5881 aaataaattt agaagataaa tttaagtttc aaggtaaact tatttaatca cagcaaatag
     5941 ttggcttact taagcttttg ggctaaactg tgagaactaa ttaagtctct gctatctaac
     6001 aaacagagca caaaaataaa aattaaatat taaatataaa gcacagttaa ttttgattaa
     6061 aaagttgact gtcttttctt ttgtagaaat ttaagcatag gtaataaaga aatgactgca
     6121 tttaagcatt atgtatataa ttggaggcaa taaaaagcca gcaaagccca taaaatagag
     6181 ctgtgctagc aacatgatga gttataagaa gataatcaaa aacttacgct ttattttaat
     6241 ttaaaaaaat gattactgta aagaaatcat tttttattat aatcactgat acatcaaata
     6301 ttctaaataa aatttttatt tatctggaat tgtatatttt ttctataata ttaactacag
     6361 cgtacattgc ccaagcacag atagccaata caaatactcc catcattact aaatctaatt
     6421 taaatacttg agaaccatat actatcaagt agcctattcc atatcttgaa actaaaaatt
     6481 ctccaactat tacacctacc catgccatac caatattaat tttagttaaa tttattaaat
     6541 ttcctgtgtt tgcaggtaat atgagtttaa ataatatttg agatttagta gctccaaagc
     6601 tttttaacat cttaattttt tcttcatcta tattcataaa ataattataa gctgaaagta
     6661 tagtaacaac aacagaaatc gttacagctg ttacgacgat tccttctatt ccagcaccta
     6721 cccaaactat aattatagga gctaaagcag tcttaggaag agcatttaaa actaccaaaa
     6781 atgggtctaa tatttttgat aatttttctg accaccaaag agcaattgct accaaaatac
     6841 ctaatacagt acctataaca agaccaagaa cagtttcata tacagatatt ccaatatgtt
     6901 taaataattg accactaaat gcatattgaa taaataaatt gtaaatatca cttggtttgc
     6961 taaatagaaa tgtatttata acatttaagt cagctaaaag ttcccagatt acaataaaac
     7021 caattaaaat taatagctgg tagaataata catttctttt ttctcttttt acacctttaa
     7081 catagtttat atatccttca gatcttttat taagcctcat gtgtatctag ctccttccat
     7141 aatatgtcga aatacttttg aaaatcagga actttacgtg ttttgagagg cgtttttgtt
     7201 ccatccatgt taagttcaat atcgtaaata ttttttatgg tagctggtct tttagatagt
     7261 attgcaacct t
//
LOCUS       DAGSHT010000171         7271 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGSHT010000171 DAGSHT010000000
VERSION     DAGSHT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.177"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1668
                     /locus_tag="MJB72_002968"
     CDS             <1..1668
                     /locus_tag="MJB72_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8264043.1"
                     /translation="VLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSYSYLFG
                     KEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYVSNIFL
                     FTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFFTNKYA
                     SLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQNIQIY
                     EELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNERNNYKN
                     ILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSKIVIDL
                     PKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLSIICQE
                     LGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQYAENI
                     IDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIFCLASR
                     DKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            1665..2117
                     /locus_tag="MJB72_002969"
     CDS             1665..2117
                     /locus_tag="MJB72_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8264044.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            2119..3174
                     /locus_tag="MJB72_002970"
     CDS             2119..3174
                     /locus_tag="MJB72_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBY8264045.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            3241..3549
                     /locus_tag="MJB72_002971"
     CDS             3241..3549
                     /locus_tag="MJB72_002971"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBY8264046.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            3653..4918
                     /locus_tag="MJB72_002972"
     CDS             3653..4918
                     /locus_tag="MJB72_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBY8264047.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            5006..5722
                     /locus_tag="MJB72_002973"
     CDS             5006..5722
                     /locus_tag="MJB72_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY8264048.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVEWVLNFSKERNINLGIITNGPSTHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENAMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(5917..6210)
                     /locus_tag="MJB72_002974"
     CDS             complement(5917..6210)
                     /locus_tag="MJB72_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBY8264049.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(6216..6431)
                     /locus_tag="MJB72_002975"
     CDS             complement(6216..6431)
                     /locus_tag="MJB72_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264050.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            6878..>7271
                     /gene="tnpA"
                     /locus_tag="MJB72_002976"
     CDS             6878..>7271
                     /gene="tnpA"
                     /locus_tag="MJB72_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBY8264051.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTK"
BASE COUNT         2734 a          759 c         1314 g         2464 t
ORIGIN      
        1 gttttgtcaa gtgaattaga aagaaaccaa ataactatta cttatgataa atcttttaaa
       61 ataagtggag aagaagacat tatatataag caaagattga acatattaaa atcgtattct
      121 tatctttttg gtaaggaaaa atctgattta agtgtatttg agaagtattt agtgaatgta
      181 attgaaaaaa tattcaaagg aatttatttg attgatatat ataattggtc tatgaattta
      241 ttattgcaaa tgggatggac tttaaatgat gaatctttta catggtatgt gtcaaacatt
      301 ttccttttta catggtatat acattcagat gaaaaacatc ctttagaatc tgcttcatta
      361 agtgagccgt gttttgaaaa taagtttgac caagaatttg aaacaattat tcaaaaaaag
      421 ttgactaagg aacaaatttt tctgttaaag agttttgtat tttttacaaa taaatatgca
      481 agtttaaacg aggatatgga tttgattagt acagaaatta tagttcaaga tctaatttac
      541 aatatgtcag aaatattaga gattagcttt aaagaagata tgattcttta taaaggatta
      601 ctcaatcata ttgccccatt gatagagaga atcaaacaga atatacagat ttatgaggaa
      661 ttacttcatg taataccaga taaatatcaa tatgtacttg aagctactaa ggtttctatt
      721 caaaaaaaca gagttttaag tcaaataaag aatgaaaatg aaacaatact tttggcaata
      781 catttcttag gaagtataca tagaaatgag aggaataact acaaaaatat attgttagtt
      841 tgtggatttg gatatggtgt aattgctatg ttaaaggata gtttagtaaa tgattaccag
      901 ataaatatag tagagagtat tccatcatat aagttacaag attataaaga atgggaaagt
      961 attgacttca ttataacaac atcaaagata gtaatagact tgcctaaaac aattattgag
     1021 ataaatccat ttttacaaga agaggattat ttgaagtttg aagaaaaagg gataaaaaga
     1081 aagaacgtct taactaatta tatgtcaata aaaaaaaggt tggacttttt agaagaagat
     1141 gcccaaaaaa gagtattaag tattatatgt caagaacttg gatacagtga tgagagaata
     1201 ttatttagac aacttaattt aagtgattta ataggtattg ataccataag gataattgat
     1261 gaagatatga aatgggaaga tgctgtaaaa attagttcaa acatacttgc taattgtgat
     1321 tttgtatcag cacaatatgc tgagaatatt atagatattt taaaaaatgt gggtttttat
     1381 gctgtaaaag ataacgaatt tgctttacta catggcaatg atagttcatt agtcaaagta
     1441 agttcgataa gtttattaat atgtaaagaa gcagttagtt ttggagataa aaaggttaaa
     1501 atcatatttt gcctagcaag cagagataaa aaagagcaaa ttcctgcaat aattaatcta
     1561 actaaaatgg tttataagac taattttatt gctatgttag aaagtgcaaa gacaaaagaa
     1621 gaagcagaag ttttaattca taaatatgag aaggaggtca ctgtatgaat aaattagtta
     1681 atgtaaaatg ttcatttaaa atacatgtta attcaaaaga agaagccatt atatcgttag
     1741 ttgatgtagt gtcaaaagaa ggatatttaa tagataaaaa ccaattttta aaagatgttt
     1801 taaagagaga agaaacattg tcgacttata ttggacatgg tataggtctt cctcatagtc
     1861 aaagtgtagg tgttaaaaac tcttgtatta caataggaaa gcttgatact cctattgaat
     1921 ggacagaaga aggagaaaaa gtggatttaa tatttttgat atcagtaaca aaagacaatg
     1981 aaaataatct tcatttaaag attttatcaa aattagcacg tttattgatg catgaaagtt
     2041 ttagaaatca aattagatgt tcagatgaac aaactgttta taatcttata aaagaaaaaa
     2101 tagaggagga agattaaaat gagaatttta aagtttttgc aaaaacatct tatgacagca
     2161 acatcttata tgattccatt tgtggttgca ggaggaatat tatttgcttt atcagtaact
     2221 ttaagtggac aagctgctgt tcctgaaaca ggctggttgg ctaaactgaa tcaaattgga
     2281 gctgctggct tggctttatt cattcctata ttaggtggat atatagcatt tagtatggca
     2341 gataaaccag gattagctcc aggtatgata ggtgcatatt tagcaaaaga agtaaatgca
     2401 ggttttatag gtggtatcat agcaggattt atagcaggat ttgttgtttt acaattaaag
     2461 aaaattaaat tagctcctac aatgagaact ttaggttcta tatttattta tcctttagtt
     2521 ggtacattga ttacaggtgg tataattgta tttttaattg gagaacctat agcttctttt
     2581 atgacatgga tgacaaactg gttaaatgga atgtctggtg tttctaaaat tcctttaggt
     2641 ggaatcttag gaggaatgat agcctcagat atgggtggcc ctataaataa ggttgctgct
     2701 acttttgccc aaactcaagt tgacacactt ccatatttaa tgggtggagt tggcgttgca
     2761 atttgtatac ctccaattgg tttaggatta gctacattat tgtttccaaa aaaatttagc
     2821 aaggaagaaa gagattcagg taaagcttca ctattaatgg gatgtgttgg aattacagaa
     2881 ggagctattc catttgcaac atcagatcca gttagagtaa taccttgtat tatggctggg
     2941 tcgattgtag gaaatattat ggctttttta ttaggatgtt taaatcatgc accctgggga
     3001 ggtctgatag tgttacctgt agttgacaat aggttggggt atattgcatc agtattaact
     3061 ggagcagtag ttgttgcggt attaatgaaa ttagtaaaaa aagacgtgaa agaagatgaa
     3121 gaaattgaag aagatttaga tgaatcaatt gaattaatat ttgaagaatt ataaatataa
     3181 atggaaggaa tttgtataaa aaagtaaata taaaactata aatttaaagg agataagaat
     3241 atgaaaattg tagcagtaac atcttgtcct agtggagtag cacatactta catgtcggct
     3301 gaatcacttg aattatcagc caaaaaattt ggtatagaaa taaaggttga gactcaagga
     3361 agctctggaa tagataatga attgtcactt aaagatattg atgaagcaac ttgtgtaata
     3421 ttgactaatg atgttgagat tagaaatatg gaacgtttta aaggaaagaa ggtagtaaga
     3481 atgagtgtat ctgagataat taagaaatct gatgcattaa ttaagaaaat aaaagatact
     3541 tttcaataaa aattgattta ttttacatat aactcaaaga tgtttttaaa gaaattttta
     3601 ttaaaagcat ctttgaaaat atcttaaaaa tagaataaaa ggagatatag tcatgaaaaa
     3661 gttaccaata aagactgtcg tgcaaagatt actaaactta caagaagagg ggaaaagtgc
     3721 aactctttta ggaattggtc ctatgtcacc aaacttgtta caagcaagtt ttgaactagc
     3781 aaaagatgat gattttcctt taatgtttat cgcaagtaga aatcaagtag atgcagatga
     3841 actaggtggc ggttatgtaa atggttggaa tcaggaaact tttacaaaag atattaagaa
     3901 ggttgccgac aaagttggat ttgatggttt atattattta tgtagagacc atggtgggcc
     3961 atggcaaaga gataaagaga gaaatgacca tttgcctgtt gatgaagcaa tggagttagg
     4021 aaaaaaatct tatttagcgg acattgaagc aggttttgac ctattgatga ttgaccctac
     4081 aaaagaccca tttgaaattg gaaaagtaat tccactagat gtagtactag aaagaactgt
     4141 agaattaatt gagtattgtg aaaacgagag aaaaagactt aatctaccag acataggtta
     4201 tgaggttggt acagaggaaa ccaatggagg tctgacttca actgagacgt atgaaacatt
     4261 tataactaga ctaaaggttg aactcgataa tagaggtttg ccaatgccaa catttatagt
     4321 tggtcaaact ggtactttaa ctagaaagac agaacaagta ggaactttta attttagaaa
     4381 tgcttatgat ttagctcaaa tggctaagaa atatggagtt ggtttaaaag agcataatgg
     4441 cgattactta gatgatgtaa ctttattaga acatatacca tctcaaatta ttgctacaaa
     4501 tgtagctcct caatatggta ctgaagaaac tagggcatat ttaaaattag cagaagttga
     4561 gtgtaaatta gaaaaagagg gcttagttga gaaaacttct aatatcagac atgttcttct
     4621 tgttaatgca attgaatgtg gacgttggag aaaatgggta gtaggagaac aaaaaaatct
     4681 aactactgaa gagatattta aggatgaagt tttatctaat gaaatactag atattgcagg
     4741 acattataca tttaataatg atgatgttaa aaaagaaata gaagtgcttt atgataattt
     4801 aagtaagaat aatattgatg gtcaaagatt tgttgtagac catattaaac gtcctttgcg
     4861 tgattatgca gaatgctata acttaaaagg tgttactacg aggattttaa ataaataata
     4921 ggacttatag tggtgagtat tgttatacta ggtatttata gttataatta tacttaccac
     4981 tattaaattt aggaggacta atattttgat taatttaatt tttgatgtag atgatacttt
     5041 atataatcaa ttaacgcctt tttacactgc atataataaa gtgttttctt caataaagga
     5101 tatttctatt gaagatttat atatgagtag tagaaagtac agtgatgagg tatttcatat
     5161 gactgagaat ggagaaatgc caatcaaaga gatgcatatt tataggataa tgaaagcttt
     5221 tgaggagttg ggaaattcta ttacagaaaa agatgcacaa agttttcaag atgagtatat
     5281 atatcagcaa tcacaaatta cactaatacc agaagttgag tgggttttaa atttttctaa
     5341 agaaagaaat attaatctag gaattataac taatggccca tcaacccatc aaagaatgaa
     5401 gttaaagcag ttaaatattg aaaattgggt tgataaaagt aatattttta tttctagtga
     5461 ggttggattt tctaagccag atactaatat ttttagagta gctgagaatg ctatgaattt
     5521 agatagagaa aatacatatt atgtgggtga ttcttataga aatgatgtat taggtgctaa
     5581 aaaagctggt tggaagagta tatggttgaa ccatagggga catgagttag aagagttgtt
     5641 ttataaacct gattttgtaa ttttggaaca taaggatttg atatctttat ttataaaaat
     5701 atgtagttat aaaaatatgt agtaaaaaaa tgttaataat agaattgtat aaaataatta
     5761 ataataacta tataaaatta tatgtttaga ttgtgagcaa tctattttat gttaaaaata
     5821 gattatttag aagaagggga aatcttaaaa aaatttgaat atcccctttt tattatttaa
     5881 ttaaaatgaa atagttagaa atatatttta tttagtttag tcaataacat tttgggattc
     5941 tttataacta catgttaact gttgaatagg tttatcatat ggtttaaccc atcctttatt
     6001 aatacttaat tctgtaagtg ctgtgtgacc ttctaccatt tgagttaatg aagctgaata
     6061 tatagctctt agttcaggtg tactacttgt caatgcagaa ttaagataca tattggcaga
     6121 tgctttagca gatgaaagca tacttaacat tattacttta tcgtctatat aagtattgtt
     6181 cttaattaga ttttctatca tatttctcat gttatctata cctcctcaga aattagtatt
     6241 tcattttcaa ctataaattg ttgtatagct ttaattcttc cttctgttgc taatatacta
     6301 gattctgatt gtctttttag atcttcatct gatattaaca tatttattgc tctttgcata
     6361 agtaatccgt cagactccat ttttataatt gctgctaaag atattttttc agtttctgaa
     6421 agtattctca tttaatcatc tcctatattg taatttatgc tttaatagta tgtctttctt
     6481 ttcaaggaaa taaccattac acttttttac agtattttat attctcgtta tatcaaaatt
     6541 tagtcgactt aatatattaa tataattttg taggatattt atcattaaat atctataaaa
     6601 taaattgttt gaatattcat aaagcaaaat ttatttgtat gtacttcttc aagagtaaat
     6661 atgagttttt tcaaaaaatt aatttttatg ataattatta agggctatat ttttcttaaa
     6721 gttgtgatta tcattattaa tttacaataa aattgccgaa aagccctctc ctttaggtgt
     6781 agggatgaaa ggtaaggtct taatataaaa aagttagaat aatgcttgta atctaatacc
     6841 gctattgata ccctatattt aggaggtgtt agtttttatg tataaatcta acaaaaatat
     6901 agtttatgat tgcaaatatc atattgtatt ttgccctaag tatcgtaaac ctgttttagt
     6961 tggcgaagtt gaaaagatgt taaaagaaat actaccatat aaagcagatg aacttggagc
     7021 agaaataata gaaatggaaa ctgataaaga acatgtgcac ttattaatat cttgtgaccc
     7081 tcaatatgga attcataaag tagtaaaagg tttaaaaggc tttagttcga gagtattaag
     7141 agagaacttt ccacatttaa agtctagtat gcctagcatg tggactaata gctattttgt
     7201 aggtactgtt ggttctgtga gtttagaagt agttaaacaa tatatagaaa atcaacaagt
     7261 taggacaaag a
//
LOCUS       DAGSHT010000172         7247 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.178, whole genome shotgun sequence.
ACCESSION   DAGSHT010000172 DAGSHT010000000
VERSION     DAGSHT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.178"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            232..1491
                     /locus_tag="MJB72_002977"
     CDS             232..1491
                     /locus_tag="MJB72_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBY8264052.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            1750..2745
                     /locus_tag="MJB72_002978"
     CDS             1750..2745
                     /locus_tag="MJB72_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBY8264053.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            2991..4058
                     /locus_tag="MJB72_002979"
     CDS             2991..4058
                     /locus_tag="MJB72_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBY8264054.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKGKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            4592..6502
                     /locus_tag="MJB72_002980"
     CDS             4592..6502
                     /locus_tag="MJB72_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBY8264055.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMLF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(6565..7188)
                     /gene="ytaF"
                     /locus_tag="MJB72_002981"
     CDS             complement(6565..7188)
                     /gene="ytaF"
                     /locus_tag="MJB72_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBY8264056.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
BASE COUNT         3059 a          716 c         1171 g         2301 t
ORIGIN      
        1 ataaatgaaa ttagttgtta taaaataaac aatttttttg ttaagaaata gtaaaagaaa
       61 tataataaaa aatatcaaaa ataaaactaa ttataagaca taaatttaca ataaagttac
      121 atttgattaa attccttgtt taacaaaagc tattcaaata taatctaagg tagttttgaa
      181 atgggaactc tattatgcaa atttaagcta gtgttaaaat gaggtgaagt tatgggcaga
      241 agaacaacaa gagtttcaag attaggtaaa gaaaaaaaac gaagtgtttt aattgtattg
      301 gcagctttag ttctaataat gggtggattt acttattttt ttaacagtaa atttctgtac
      361 aatgggaaaa tcgctaaaaa tgtatatata gaaggagtaa atgtatctga tatgactaaa
      421 gcagaggcat taaaagctat aacagataag tatactccag aagatttaaa tcttgcttat
      481 gatgggaaaa aatatacaat atcaccaaaa gatattgatt taaaatatga tacagaagat
      541 gttgtaaaag atgcttatga aagtacaaaa aaagggtcct attttcaaaa cttaaaaaaa
      601 tatattgata taagagtgaa taaggcaaat atgaagataa aagctgaata tgatgaggca
      661 aaacttagtt ccaaagtgtc ttctatagca gatagtataa atgttaagat gaaaaatgct
      721 tctattagtg ttggaagtgg tggactaagt tatacagact ctgttgtagg tagagaattt
      781 gatttggcag caaataaaga gtctatatat aatatgataa aaaataaaga gcataaaact
      841 ttagaactta aagtaaatct tcaaaaacca gatataacaa ctgaacaagt aaaaactgta
      901 aattctgtta taggtcaata ttcaactact tattcacaag ctgtagaggg taggtcatat
      961 aatgtaggat tatcagcaag aaaaactagt gatgtattat taatgccagg tgaagaattt
     1021 tcatacaata aacttacagg acctagtaat aaagcaaatg gttacaaaga tgcacctgtt
     1081 atagtttatg gtaagctaga acaatcagca ggaggaggtg tttgtcaaac atcttcaaca
     1141 gtttataatg cggctcttct ttcgggtatg gagataactc aagttacaaa tcattcatct
     1201 gcatctactt atgtgcctaa agggagagat gctactgtga gcgatggagg attaaactta
     1261 aagtttaaaa atccatataa gcatcctgta tatattaaaa attatgcagg tggaggaagt
     1321 gtatcatcag taatctatgg taattctggt gacaaaccaa atatatctat agaggtaaaa
     1381 caaactggtc agaataaata ttctacatat cgaatattta aagattcaag tggaaaggtc
     1441 ataaaaaaag aacatatatc aaatagttct tataaagaat taaaaaaata gataattttt
     1501 tagagaaata tttgtaatat aaaagggagt gtctcaaaat gaacttttta gtttatgaga
     1561 cactcttttt ctgtgaaatc aaatatattt tcagcatttc aacaatacaa aagaagcata
     1621 tctctatttt gagacagcct tttttgtaaa aattaacact attagttctt gttttaatat
     1681 tacaatcatt aatatataga aaatattcct atatattggt ataattgtaa atagatttag
     1741 ttatataaaa tgtatgagga ggtgacatgg ttgaatttta taggcaatag atatgaggta
     1801 gtaaattctg acgacgtttt agaaattaac aaaatctata aagctagaga tgttttttac
     1861 aggaaaaatg tgttaataaa agttataaag cacaataatt atatctgtga agattttgtt
     1921 tcaaatttaa ttgatgaaag tactgcttta gatgagataa actctcctta tatactaaaa
     1981 ataatagatg ttgggattca ctgtacagaa gaaacaacat tatattatgt tgtaagtgaa
     2041 gactttaatg gtatcggact agatgaatta attctaggta attatcttca tttagaagca
     2101 attgtaaaca taatgataca agttttaaaa gctttagaaa tgatacatag gaatcattca
     2161 tatcatggta gtttaaaatc gagtagtata atagtagata cagaatacaa tataaaaatt
     2221 tgtgattttg gaattacaaa agcaaataat ggggttaata caagaagcta tggaaacagg
     2281 agatatttat gcccacatca actatgtata aattatactg ataaagaaag tgattttttt
     2341 gcaacaggtc ttattctatt tgaatctata tttaaaaaat tgccttttgg agaatcaaat
     2401 tcagaagaaa aaatgctaca atctattgat aagggattag attggaatag tataaaagca
     2461 attaatggaa atactgaact tataaatgtt ataaaaaggc ttcttggaag aaataataag
     2521 tatactagag ccaatgatat tattattgat ttaagtaaag taatgtatga aaaagcatat
     2581 atagaagttg aagatgagaa agaagaagag cagaaacaag acaaagttat acaggttaaa
     2641 aaagaatata aaagtaaaat gagaaaaaaa ttacataaaa aacttgttgt tgccggtcta
     2701 gctatagtca tgatttcaat gattattatt agttccataa tataaaaatt gtatattaag
     2761 tattttaaaa tcaatgtcta taaaggatat acatagaaat tttagtgtag tcttaaatta
     2821 tgaaaggaag tggttattat gttaacaaaa aataatgagg tattaaacgt ctaggagtat
     2881 tttaacaaat agccacctgc ttttacagtt tagaataaaa aagctgtggc taaaggtcac
     2941 agctttttgc tactttttaa aggcaggaga atattaaaaa ggagcaaatt atgagcaata
     3001 taaaaataat agcaagtaat aaatgttgga ttgaagatat agcaaggaaa caacttgaag
     3061 atatttcaaa gttaaatgga attttaagag tagtaggatt acctgactta catgcaggta
     3121 aaatacctgt aggtttagca gttgagacta aaaatattat ttatccacat ataattggaa
     3181 atgatatagg atgcggaatg actttattta aaactggggt tctaaaaaag aaatttaaaa
     3241 aagacaaatg gattaagtca cttagtaaga ttaaagattt gtccgatata gaaataaaaa
     3301 acacatataa agaagaatgt ccaattttaa atttaggaac tatagggagt ggaaatcatt
     3361 ttgtagaaat tcaatgtata agtgaaattt ataataaaga acaatttgaa caattaaaat
     3421 tttcatctga tgatattatg atgttagttc actgtgggtc aagaaattat ggagaagata
     3481 ttttaaaaaa attttatgat aaagatggac ttgaagttga aagtgagaaa gctatagatt
     3541 atattaaaaa acatgataat gctttaatgt gggcagagcg taatcgtgaa gctatcagta
     3601 aaaaaattat gcagtcaata ggtacatctg gagatgttga aacaatattc tctataaatc
     3661 ataattttat agagaaaagg gaagataaat ttatacatag aaaaggtgct gtttcaagtg
     3721 aaagaggagc tgtgattata ccaggttcaa gaggcagtct atcatatata gttatgccta
     3781 ctgaaaatac agagatatct ttatattctc tatcacatgg agcaggtagg aaatggtctc
     3841 gttcagtttg taaatctcgc cttaaaggta agtatagtaa agatactata aaacaaacta
     3901 aatttaaaag tcagattata tgtaatgatg taaatttatt atttcaagaa gctccagagg
     3961 catataagaa tatagagcag attattgaaa gtttgataga atatgaactt attcaagtag
     4021 tggcaactat gaagccattg attacttata aaggataatt tttaaagtta aaaaagatta
     4081 aaattatatt ataaatagct aattttatgg aacatttaaa agtgttttat tattagctat
     4141 ttttttattt tgtagagacg ataatttata gagctaaatt ctttgatata taatgattac
     4201 aaagttagac tgtattggaa actgcacata catgaattga tgacaatata agtgattccc
     4261 aaatataaat gaaagtataa gcttattttt actataaaaa ttttcactgt acaaaaacat
     4321 taatgacatt atttttattg acataaatat ataatttatg gaataatatt agaagcactg
     4381 atataagtgt ttaaataaga atagaaaagg agaagtgttt tatgaaaaaa aatactaaca
     4441 ttaatgagtc cggagttaaa cttcttaata aataaggagt ttgatatata ataactcctt
     4501 atttattaag ttgttcgcaa aataactatt atcaaagtaa atgatagggt gatttaaaag
     4561 acgataactt ataaataaag gagaaaaaat aatgaatagt aatacatttg aaagattaca
     4621 actaaatgaa gttaaagaat taataaaaat acattgtgta agttcactag gaaaaaattt
     4681 aatagataaa ttaactccta gtgggaatat tggagtagta agaaggaaat tagcagaaaa
     4741 taaagaagct agaaagataa ttgaaaatag taatcacata ccacttgaag gattgtttaa
     4801 tgcaggttct acaatagata aaattgaaaa aggaatgata atagaacctg ttgaacttgt
     4861 aaatattgaa gattttttaa gaggttgtag gaagatgaaa gcttttatgt tagaaaaaga
     4921 attttattca cctacattaa gttcttatgc cttaaatata acagaatgta aaagtataga
     4981 agatgagata aattactgta taaaatctaa taaagttgat tctaatgcta gtaaagaatt
     5041 aaaaaagata agacgaaata tagaaattac agaaggaaag ataaaagata gattaaataa
     5101 atttataaca tcaactgtaa ataaaaagta tatacaagag tttataataa gtaaaagaaa
     5161 tgatagatat gtaataccaa ttaaatcttc ttataaaaat gaagttaatg gtactatatt
     5221 agatacatca tcaaaaggaa atactgtatt tattgaacct attagtgttt caaatttaag
     5281 tacagagtta actatgctaa aagctgatga aactattgaa gaatacaaaa tattatctta
     5341 tttaacagaa cttatatttg agaagataag tcagataaaa ttaaatatag agatactttc
     5401 agaatacgat atgttatttg caaaagctaa gtatagtcaa aaaataaaag gaattactcc
     5461 aaaaataaat aataatggct atataaaaat aattaaaggt aaacatccac ttctaacagg
     5521 agatgcagta cctttagatt ttgaaatagg caaaaattat agaagtctta taataacagg
     5581 tcctaatgca ggaggaaaaa ctgtaaccct taagactgtt ggtttactaa ctttaatggt
     5641 tcaatgtgga tttgatattt ctgcaaaaga aggtagtgaa tttagtgtat ttgaaaaagt
     5701 ttttgtagat ataggggata atcaaagtat tgaaaatgcc ttaagtactt tttcatcaca
     5761 tataaaaaat attgcagaga ttatgagttt atcaaataat tctactcttg ttctgtttga
     5821 tgaaatagga agtggaactg aacctaatga aggagctggt ttagcaatat cattattaga
     5881 agagttttac aaaatgggat gtataactat agcatcaact cattatggag agataaaaaa
     5941 gtttgctaca ttgcatccag aatttgaaaa tgctggtatg atgtttgata aagaaacgtt
     6001 agaaccattg tataagttaa ctattggaaa atctgaagat agtaatgctc tttttatttc
     6061 taaaaaaatg ggaataaaaa atagagtttt agatagagct aaagaatatg taattgatag
     6121 aaactacaat ttagatttag ttaaaagaag taagctacaa tctaatattg aagataaaaa
     6181 tagttctgaa gtcattttaa actatacaga ttataatgta ggggataagg taaaattact
     6241 agatgaagat gattttggaa ttgtatataa gcctatggac aagtttaata atgtagaagt
     6301 attgtttaat gataatttta tttcagtaaa tataaaaaga ttacaactta gcataaaagc
     6361 atcagaattg tatccagaag ggtatgattt agatgtatta tttactagtt ttaaagaaag
     6421 aaaattagaa aaagatattc aaagaggttc aaaaaaagct cttaaaaaga ttcagaaaga
     6481 aattagagag aatagaaaat aaatataaaa agcacaaaag aaagcaatct aaagagaaaa
     6541 gtttatcttt agattgcttt cttattaaca tatcttcata aatccaatga ctaacaagat
     6601 taaaccactt aaccaagata agtttaaatt tacgttttta acaaacatat ttcctagtaa
     6661 acaaccaagt aatactgcaa ttatatttga tattaaagaa aatagaataa cttgtatata
     6721 attcacacct acaaggccac taccaaaacc gatagctaat ccatctaaac ttaaagcaag
     6781 tgcaagtgta aatgcttcag cagatttaag cactttagat ttacttgtat ctaagacatc
     6841 aggttcgata cttacattaa aatttaattt aaggtctaac atattaaaat tataattctt
     6901 tgagtgtata gattttttct taaaataaga tttaaaaaaa ctttcaatta gtctagcact
     6961 tcctaataaa aataaaataa agaaacatat taacattgtt atattttgag gtaaaaaatc
     7021 tctaactatt gtacctgcaa aaagcgatat tgctaaaata gctgaacaaa ctacgctgat
     7081 aattatattt gaatataagg gaactttaac tttatttaca ccatatccaa agcttgtcac
     7141 aaaggcatct atacaaagag aagtaactaa taaaattgat tcaatcacag tagattaaca
     7201 tcctttctct taaattttgt acttatatta gttatagtat tgtaaaa
//
LOCUS       DAGSHT010000173         7174 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGSHT010000173 DAGSHT010000000
VERSION     DAGSHT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.179"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            123..1004
                     /locus_tag="MJB72_002982"
     CDS             123..1004
                     /locus_tag="MJB72_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBY8264057.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1065..2273)
                     /locus_tag="MJB72_002983"
     CDS             complement(1065..2273)
                     /locus_tag="MJB72_002983"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBY8264058.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VREDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2346..2598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2750..3739)
                     /locus_tag="MJB72_002984"
     CDS             complement(2750..3739)
                     /locus_tag="MJB72_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBY8264059.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            3891..4331
                     /locus_tag="MJB72_002985"
     CDS             3891..4331
                     /locus_tag="MJB72_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264060.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4658..4885
                     /locus_tag="MJB72_002986"
     CDS             4658..4885
                     /locus_tag="MJB72_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY8264061.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4911..7031
                     /gene="feoB"
                     /locus_tag="MJB72_002987"
     CDS             4911..7031
                     /gene="feoB"
                     /locus_tag="MJB72_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY8264062.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
BASE COUNT         2559 a         1036 c          987 g         2592 t
ORIGIN      
        1 gtcatataag ttacacttta gttatcttag ttacaacttt gtcggtttac tttttctact
       61 ttcttcgata taattataat atatttttga tacaattcga caaaaggggg aatgctgtaa
      121 ttatggtaaa aaaaagaaaa aagaaaactg ttacacttac tatacttgtt gtaatagtta
      181 ttggtatttt ttctatggca tgggcggaca tgtctagaaa aaaagaaatg aaagaaactc
      241 aagaaagact taaagctgaa agattagaaa aagaaagaat tgaaaaagaa aaaaatgaac
      301 ctattattgg tgcaaaaaaa gaagctgtaa aagaatatgg atatgacgca aaaatagttt
      361 gggacaaact taacaaatac gactattcaa acaatggaga aaagatagtc tttcttacat
      421 ttgatgatgg accatctact actaatactc cacaagttct agatatcttg aaaagacatg
      481 gtgttagagg aactttcttt attaagggtg attctcttga aagaaaagga gctaatgaaa
      541 ttttaaaaag aacatttgac gaaggaaatg caatagccca tcactcttat actcatgact
      601 ataaaaaact atatcctaac agaagcctaa atctcgatac attcgtaaat gaacttaaca
      661 aaactgatga agctatgaaa aaagtcttag gaaagaattt ttcctcaaat gtagttagat
      721 gcccaggcgg gtatatgtca tggaaaaata tggaacctct aggaaactac ttaaaagaga
      781 aaaacatggc atcaattgat tggaacgctt taaatgctga tgcagaaggt aaaaagaaaa
      841 atgcacaaga attatttgaa catgcaaaga aatcttctga aggcaaagaa atggttgttc
      901 ttttaatgca tgatacttat ggaaaacaag aaacagtaaa tgcattggac caaattataa
      961 catattttaa agacaatggt tatcaattta aaattttaat ataataaaaa agcaagttta
     1021 ttgaatgtac attttatact caatagactt gcttttactt tactctattt tactagctcg
     1081 atttttacaa attttttctt tcctatttga acaactaatt cactctcttt tacaatagtg
     1141 cttaaatcct ctactttttc accatttacc tttactccac cttgagaagc taatcttcta
     1201 acttcactct tactcttaac aatctcatta gaaactagta cttcgattaa gtcaaatcca
     1261 tcttccctaa cttgaattgt ttgtatatct tcaggtattt gacctttttg gaatacagat
     1321 ttaaatcttt cttcagcttc tttagcagat tcttctccat ggtataatgt tactatttct
     1381 cttgctaagt tcatctttat atctcttgga tttactgaac cctctttcaa ctgagattct
     1441 attttattaa cttcatcagg atgaacatct gtcactaaat tgtagtattt aattataagt
     1501 tcatcaggaa tttccataga tttttggtac attattcccg cttcttcatc tattccaata
     1561 tagttaccta aactcttact cattttttct ttaccatcta atccttctat taaaggcatc
     1621 attattacta tttgagactc cataccaaat tctctttgta atgatcttcc cattagtaaa
     1681 ttgaatcttt ggtcagttcc accaagttct atatcagctt ctaatgctat agagtcatat
     1741 gcttgcatta atgggtagaa gaactcatgt actgatattg gcatttgtcc ttcatatctc
     1801 tttttaaaat cttctctttc taacattctt gcaacagtaa tagttgctgc taattttatt
     1861 acatcttcaa aatttaactt agctaaccac tcgctattaa atcttacaat agttttttct
     1921 ttatctaaaa ctttgaaaat ttgttcttca taagtttttg catttgcaag aacttgctct
     1981 gttgttaaag cttttctagc tttagatttt ccagttgggt ctccaatttt tcctgtaaaa
     2041 tctccaatta ttattactat ttgatgacct aaatcttgta attgtttcat ctttctaaga
     2101 actactgtat gccctaaatg tatatctgga gcacttgggt ctagacccaa ttttactatc
     2161 ataggctttc cttctttttc tgatttaata agcttttccc ttaactcttt ctcatctatt
     2221 aaatcatcta cacctttcat aattattcgc atttgctcgt ctatcgattt catttttcat
     2281 tcctcctaaa atatttatat aatctataat ttttattttt ataataaaaa aagctttcat
     2341 cctcttaaaa ggacgaaagc attgcttacg tgttaccacc ttagttcatc aatatctcac
     2401 gatattaatc tcttagagta caattacata tactcgtgta ctataacgtg tactaatccg
     2461 atatctctta atggttgtta tctttcagag atagtgctct gagatgtatt caaactaaat
     2521 caatttattc catttcacca aactggaact ctctgttaaa taaaatatag ccctacttct
     2581 tctcttcata gcatttatat attcagatta tttcatcaat tacaagaaat ttttcttaac
     2641 ttactcataa tatattatta tttttatatt ttgtcaacct tttttattat tatatacatg
     2701 tttttttaca ttatactact tttttatatt ttttaccaat acccaaaatt tatttttcaa
     2761 tcaaaagatt tatttgttta ttgactatat tactctgact caatcttata tttattttct
     2821 tatttaaaaa tgcgtttata attattaaac caataactgt tacatcaaat ataaaaaaca
     2881 gcaataaaat tattatcaaa aataaaattc tagcatataa taattttcta aacttagcta
     2941 ttataaagtt ttgtagtgac atacatgagt ctctaatgat aaaatagaca cttatatagg
     3001 ttattgatat aaagattgtt tttaaataaa cagaatcaac tttaacattc aacagtttta
     3061 gaacttcaaa aatggctaga taagaaacac atatgtaaaa agcttttttt gagcaacata
     3121 aaaattggtt taaatttcta aaattcttaa atattaaaaa tttttttaaa gaattagaat
     3181 ctaaaatatg caatttattc cctaaaatta aacttaataa atatatgcaa aacaccatac
     3241 caaaaggaaa caatgctatc attaaataat aaataaaatt tgtataccca ttaaaataaa
     3301 tccatttgct atatccttga aattctttca taaaactata acctattaat gttgaagcat
     3361 atatatcaat gaaaaccatt acaataaccc caaatactga accatatact aaatcatcca
     3421 taaccattgt aggcttattt attagatagc cacacattat accaagtaat acactctgac
     3481 ttgcccaaat agcagttcct agatttccaa gtaccagaga aattattaaa agcgaagtaa
     3541 ttccagataa aatggtatac tttaaatcac atttcaagca gattatacaa aaaaatactg
     3601 gcactataaa atctaagtaa atagcatatc caaacccagt actcacagct ataattgtta
     3661 ctactatgaa taaagatgac aataaagctg cttcagttaa ttttttagta gtattcaaaa
     3721 cactgcctcc ataaatcata aattttctaa ctaaagtttt gtttttaata tagtatatca
     3781 ctttttaaaa atatttttaa taaatataaa ttttaataaa ggtatttatg ttaaaaatgg
     3841 tataatgtat cttaatattc agttttttaa gatgctagga ggtaattact atgtttaaaa
     3901 ttaaacactt taatgatttg agtttagatg aattttatga aatagcaaaa tcaagatatg
     3961 aggtatttgc ttgtgaacag aagatatttt cattaaacga ttatgatgat atagataaaa
     4021 gctcttatca catattttta aaagaaaatg gattgatttg tgcttacgct agaataattc
     4081 ctaaagaata cagctcttat aatgatgttt ccattggtag agttttagtt ctttctagtc
     4141 atagaagaaa aggattagca aaacaaatga tggattgtgc tatagatttt ataaaagtta
     4201 acttacacga aaataatata accttatcag cacaaactta tataaagaac ctttacttat
     4261 cttgtggttt taaagaaatc tcagaagtat acgacgaagc aggtattgaa catattaaaa
     4321 tgagattata gtgttgacaa gctaatattg ttaatatata atttaagtat aaattatata
     4381 ttgaattgat atttaggtat ttgaaaatac cttacataga gtcagaaggc ttttgctttc
     4441 tgactttttt attatcaaaa ttcaataatt atatggattc aaagtgttaa tttttattct
     4501 atattctact ttccccaaaa gtaagaatta tacccaactt tgtatgatag gcatcttgcc
     4561 tatctttttt ttgtaaaagt aacaaggtaa taatataatg catagtttaa aataagaaga
     4621 ttagcccagg ctaaatttca agcatggagg tgctattatg aaaaacctta acgatattca
     4681 agtgaaaaaa actgtcaaag ttgaagatat attatctagt ggtaatttaa gagaaagaat
     4741 gttagcgctt ggtcttacta gaggagctgt tatagatgta gttagaaaag gtccaaaaaa
     4801 taatttaact gtctataaaa tcagaggtag caagatagct ctacgacaag aagaaagtag
     4861 cttaatatta gtttctgaca tttaaaaatt tctagcagga ggatattaat atggggttga
     4921 cacataactc aactaaaatg agttctttaa aagacatgtt tgatatagac aataaggaag
     4981 accagtttgt aattgcattg gctggcaatc caaatacagg taaaagtact gtatttaatc
     5041 atcttacagg gcttagacag catactggaa actggccagg aaaaactgta gctacagctc
     5101 gtggtaattt taaatataaa aataccgaat atgcattaat agatttacca ggaacatatt
     5161 ctttatttgc cttatcacaa gaagaaattg tagctcgtga ttttatatgt tttggtaatc
     5221 cagatgcagt aatagtagta tgtgatgcta catgtcttga aagaaattta aatttagtat
     5281 ttcaagtgat ggaacttaca gataaagtta ttttgtgtat taatttaata gatgaagcta
     5341 gaaaaaaagg catcactatt gataaaaaac ttttagagga ttctcttggt attccagtta
     5401 ttttaactgc tgcaagaaat ggctctggta tggatgaact attggatact ttaaatgatg
     5461 tatcttttga caaatataaa ttaaataata aacctgttag atacaatgaa aatatagaga
     5521 atgtggtaaa atcaattcaa ccagaattgg ataatattat accaggtata aattctaggt
     5581 ggttgggact tagacttatt gatggcgatg aaagtatatt tgaatctatg agtaattata
     5641 tagacaaaga ttctatagat gctattaatg aagtaaagaa aaagattcca gacaatataa
     5701 ataaacagaa aataagagat gagtttacta aaatcaacta tgattatgct aaaaaactga
     5761 gtgatgagtg ttgttctaat gttgctaaaa aatcaactga tagagaagaa aaagtagata
     5821 aaatattaac atctaaaata tttgggcttc caataatgct cttattatta ggtactatat
     5881 tgtggattac tatagaagga gctaactatc catccacttt attatctaat ttattactcg
     5941 gatttgagcc aagtatttcg ggtatattaa atagtattaa ttgtccatca tggttaaatg
     6001 atatgttggt tttaggatta tacagaacat tagcatgggt aatctctgta atgctacctc
     6061 ctatggcaat attttttcca ttatttaccc tacttgagga ttttggatat ttgccaagag
     6121 ttgcatttaa cttagaccac ctatttaaaa aagcttgtgc acatggaaaa cagtgtttga
     6181 ctatgtgtat gggatttgga tgtaatgcag ctggagttat tggttgtagg ataattgact
     6241 ctccaaggga aagacttata gctatactta caaacaactt tgttccttgt aatggtcgtt
     6301 ttcctacttt gatagctata tctacaatat tttttagctc agttataacc aactctttcg
     6361 tatcaagcgt agcaactgca ctttgcataa ctttactgat aattctaggt gttataataa
     6421 cattgttggt ttcttatact ctttctaaaa cgctattaaa aggagttccg tctacattta
     6481 cattagaact tcctccttat agagttcctc aaataggaag aactttatat acctctataa
     6541 tagatagaac aatatttgtt ttaggaaggg ctgtaatggt agccatacct gctggtgtca
     6601 taacttggat atttgcaaat atatatatag gagatttaag tatattatca catgttgcaa
     6661 actttttaga ccctttagct aaattaattg gtcttgatgg ttttatatta cttgctttta
     6721 ttcttggatt cccagcaaat gagatagttg tacctatact tttaatggct tacctagcaa
     6781 ctggctctat gatagaatta gatagcttta gtgctttagg tcaagttctt agagaacatg
     6841 gatggactta tttgacagct ttaaatgtta tgctgttcag tttactgcat tggccttgtg
     6901 caacaacact tttaacaata aaaaaagaaa ctggtagttt aaaatggact gccttaggat
     6961 tcttaatgcc tactatactt gcttttgttg tttgtttttt aacaactaca gtttacaatt
     7021 tatttatata attagtatta actgacagta gacatatatt agagaaacta tttaaaaatt
     7081 tatatacttt attataaagc atagagatat attcaatgat aagtaaaatt acttaacatt
     7141 tgaatatatc tctattttaa ttcttatctg gata
//
LOCUS       DAGSHT010000174         7040 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGSHT010000174 DAGSHT010000000
VERSION     DAGSHT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.180"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            95..2140
                     /locus_tag="MJB72_002988"
     CDS             95..2140
                     /locus_tag="MJB72_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8264063.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2401..3600
                     /locus_tag="MJB72_002989"
     CDS             2401..3600
                     /locus_tag="MJB72_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBY8264064.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3652..4347
                     /locus_tag="MJB72_002990"
     CDS             3652..4347
                     /locus_tag="MJB72_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBY8264065.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4364..5041
                     /locus_tag="MJB72_002991"
     CDS             4364..5041
                     /locus_tag="MJB72_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264066.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5152..6753
                     /locus_tag="MJB72_002992"
     CDS             5152..6753
                     /locus_tag="MJB72_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY8264067.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2830 a          693 c         1310 g         2207 t
ORIGIN      
        1 caaaatttga tttttaagat ttaaaacttt tttaaaaagt gctacaatag accaaattat
       61 tactttctat actcgttaaa caggaggtgg gactatgaaa aaacataata ttttatttgt
      121 atcaactgat gacaaaataa atatagatat atcaaaacaa ttggaaaata tatttggaga
      181 attttgtaat atagataact tggtatatgt aaataggata aatattgaac tgtcaagcta
      241 tgagcttgta gtatgttcag acaatgatat aaaggaatat atacataata atattgataa
      301 aaatatacct attgtaatag tacatcgtac tataaatatt gaaaatataa atcagataat
      361 aagtatagaa aatgattctg atgttatggt tatagatgct tataaagagt cagcagatga
      421 aactgccaag ataattagaa aactaggatt aattcatatt aatctaatac catattatcc
      481 aggttgtgat aagtctaaat gtgagattgg aattattaca ggaagcagaa acagcatacc
      541 acaaaacata aagcaaataa ttgacatagg tgataaggta attgatataa atacagttat
      601 agaaatcttt acaaaactaa atatatctat agataaatta catattatta aggaaaaata
      661 caatgaagat acagtaagtg gatataggta ttatacaact atgaataaaa ccatgaagag
      721 ttttttggag attatagatg aaggtattgc ttctgttgat aaattaggaa aatttatata
      781 ttgcaacaaa gtattttcta atctagttgg aatagaccaa aatgaaatta tatctaataa
      841 ctttatggac ttatttagtg ataaggttgt caaaaaaata ttttttcaag aagatgaagt
      901 taatgatgaa gtagtaaatt taaataacaa aaaactgata attaataaag taaatgttta
      961 tgaaaataat gaacgaataa agagtattat aagcattaaa aatataagtg caattcaagt
     1021 gcttgaggac aagattcaaa ataaacttca gtcaaaaggt tttgtatcaa agtatacatt
     1081 tgagagtgtt gtaggtgaga gtaaaataat aaaagaaaaa attaatattg caagaaaaat
     1141 agcaactaca gacttttctg tgttgatact tggagaaaat ggaacaggaa aagaaatatt
     1201 tgcccaggca atacataatg aatctctaag aaaaaacaag ccatttgttg cagttaatct
     1261 atcttctttg tcagatacac ttattgaaag tgaacttttt ggctatgatg aaggaagttt
     1321 tacaggagca ataaaaggtg ggaaaatggg tatatttgaa agagcacaca caggaacaat
     1381 ttttttagat gagataggtg atatatcttt agatgtacag cagagattgc taagagtatt
     1441 acaggaaaaa gaagttatga gaatgggcgg aagtaaaatt ataccaatag atgttagaat
     1501 tatagctgca acaaataaag atttaaagaa aaaaattaaa gaaggttcct ttagagaaga
     1561 tttgtattat aggattaatg tattacatat agagatacct agacttagag aacgtaagga
     1621 agatatacct cttataagta agtatttttt agatgaaata aatagcaaca aatgttttac
     1681 agaggaatca atgcaagctt taacattgta tgaatggcca ggaaatgtaa gagagcttaa
     1741 aaatttaatt tattatattg atactatagt tgaggaagat agagtagatt acgagcattt
     1801 gccagaacaa tttagatttg aaaaaaacaa tactttagtt aatgaaaatt tcgattcaat
     1861 tatcttagat ttcaaacaat ctaatttctt tgaggaaagt atatgtatat tgaccagtat
     1921 agaaacttgg aataataaaa atatattatt aggaagaaat aaactgcaag agattttaaa
     1981 agaaaaagat atagttttaa gtgtagacca aattagaaaa agaatagata agttaaagag
     2041 ccatggttta cttttatctg gagtaaaaaa acaaggtagt tttataactg atgaaggaaa
     2101 gaactttata tcatatgtaa aaattaaggg agtgatatag gggactaaaa aggcaaaaca
     2161 ccctaaaaaa atccctataa tggatagaaa taaataaaat tatctttaag attaagataa
     2221 ttcaaaaata ttgaataaat aatctaagaa gattattttt aataaaacag tagtaaatat
     2281 caaccttact actgttttta ttttttgcaa gatttattat acaattaaaa tttatatttt
     2341 ggcacatcaa ttgctacata ggaaaaatat aaaaaattgt aaaacttgga ggatgttgtt
     2401 atgaagtcag cttacaaagg atatgaagcc tatagttatt tagaaaaagg aaaagatatt
     2461 tcatctattg agatgtgtaa aggagataaa agagttaaag aataccttat tgaattagat
     2521 gatattcaag aagctaaggt taaaagtata gtggatgaaa aaatatttat atcattacat
     2581 gaacatccag tattatttcc agaacaccta gaaagagata tatttgaata taatagggaa
     2641 ggtaaacaaa gatgtgctta tgaagcatta tcaagaggat attatgatgc tatatttgat
     2701 aatttaatgg atggtgtatg tacgataaca tcaaattcag ggtggaaatg ggatgatata
     2761 ttaatagatt taggcatgag attatgtgat ttagctcatc aagattttgt cataaagtgt
     2821 gaaaaagtag aagatatata cagagctaaa agtgaaggta aagtagcatt gataccaaca
     2881 ttagaaggtg cagcaccaat agaaaatgaa cttgatagaa tagatatact gtatggattt
     2941 ggtataaggt taatgggtat aacatacagc gaatccaatg cattaggttc aggactaaaa
     3001 gaagagaaag atggaggtct tacaaatttt gggaaaaaag ctgttgaaag aatgaataaa
     3061 attggaatgg caatagattg ctctcacgtt ggagttcaaa ctacacttga tgtaatagaa
     3121 tatagcaagg acccaataat attaagccat gttggggcaa gaaatttatg gaacacaaag
     3181 agactaactc cagatgaagt attaatagca tgtgctaaaa aaggaggggt tataggtgta
     3241 gaagcagcac ctcatactac aataacagag aagaataaag aacattcaat agagtcattt
     3301 atggaacatt ttgagtatat taaaaattta gtaggaatag accatgtagc ttttggacca
     3361 gatacagtat atggcgacca tgtaaaatta cactcagtac tagctgctaa atttgatata
     3421 aaaaaaataa caggaacaaa agaacatccc aaagtagaat atgtaaaagg tcttgagaac
     3481 ccaacagaaa cttcaaaaaa cattgttaga tatttagtaa agcatggata tagtgatgag
     3541 gaaatagaga aggtactagg tggaaatata cttagagtac taaaacaggt ttggaaatag
     3601 atatattaga aatattaata aaatttgagg atttataggg aggttttata aatggattat
     3661 ttaaaattag ctaatcatcc acttttgtgg atagcgagta cagtagcagt atcaatagta
     3721 gtggtacaat cccttatatt tgctataaaa tcttgcaaag ttggtaaaac aatgggaata
     3781 acagataaac aaataaagtc agctgttaag agtagtgcta tttcagctat tgggccttca
     3841 atgactatat ttgctggaat ggtatcgctt atagtaacta tgggtgggcc tatagcatgg
     3901 atgagattat catttatagg ttctgtaatt tttgaatcaa tgtcagccgg atttggaaca
     3961 gatgcattag gtataacttt aggttctcct gaaatgacaa aacttgcatt tacaaatgca
     4021 gtttggacta tgatattagg ttctttagga tggataatat tcacattact atttggacat
     4081 aaactagata agataaccaa tgttatatct agtggcaaga aatcatttat accaataata
     4141 tctctaggag caatgttggg ttcatttgca tatctaaatg ctgatagggt gttgcgtttt
     4201 gataatggaa caattgcatg tataagtgga atggctataa tggtaatatt gtgtatgctt
     4261 gaaaagaaga aaaatataaa atggttaaga gagtgggggc ttacaatatc aatgtttagc
     4321 ggaatgataa tagcttcagt aatataaggg gggagttagt tttatgagta aagtaaaatc
     4381 tatatacaat gaagaatatt taccttttat gataaggtat ggaagattaa cactatcttt
     4441 gggtattata gcagcattag ttcctggaat aattttgagc tttggatttg gtattatgcc
     4501 tccaatatca gcattattag caagtacaat ggcaattgtc agtatgagtg ctccaaatta
     4561 cataatagaa ccagtttctt attcaccaat cttaggtata ccaggaactt atatgagttt
     4621 tctttctggt aatatatcta atatgaggct accatgttca atagcagctc aaaaggcagc
     4681 agaagttgaa tcaggtacag aagaaggctc gataatatct acaataggaa tagctgtatc
     4741 tattttagtg aatataagta tactaactat aggggtaatt ttaggaggaa gcgtactatc
     4801 taaaattcca gctgaagtag tagaaaaatt aaacttaatt ttaccagctc tatttgggtc
     4861 agtttttgga caagtatttt tacaagataa aaaattaggt ctagtagcaa ttgtaatatc
     4921 tgtattgact attatattat caaagcaagg tataatacca cagtctctag tagttcttat
     4981 atgtgtattt ggtactatat taattgcaag agctatgtat aaagataagc ttagtgacta
     5041 attacaatga aataatagtg aattttcaaa tatgaaattt aataatttga ttttcaaata
     5101 taaaaattac attaagatta tttaaaaatt ggtttttaag gagggaatat tttgatatat
     5161 gatttaatct taaaaaatgg atttataata gatggtacag gtaatcctgg tttttatgga
     5221 gatatagcaa ttaaagacaa cttaatagca aaaatagatt ctaagataaa tagcaataca
     5281 aataaagaga ttgattgttg tggaaaagtt ataacacctg gatttattga ccctcatgtt
     5341 catgaagaaa tagtagcaat actagatggt aaatttgaaa agtttctaaa acaaggagtt
     5401 acaacaacta taaatggtaa ttgtggacat tctataacac catattcttc tgaaaatgtg
     5461 tatgaatata tgtataaaaa tggtctatta cttgaagaag aaaaaaagta tcttatagat
     5521 aaaaataaat gttggaataa ttttacagaa tattgtgacc ttatatctaa aagtggtatt
     5581 tctgtcaata tgggattctt gttggggcat gggactataa gatggagtgt tatgggaggt
     5641 tctaaggata gacctcctac agaaaaggaa aaaaatgaaa ttacagatat aataaatgat
     5701 ggaatgaaaa gtggtgcttt tggaatatct actggattgg catatatacc aagtaagtat
     5761 gctgatattg atgaacttgt tgatattgca aggcagatta aagagtatga tggtatatat
     5821 acatctcata ttagagatta cataggaaga tataatgcag taaaagaagc catagaggtt
     5881 gggcaaaaaa gtggtgctag ggttcaagta tctcacttaa gtccagttga aatagaagca
     5941 tttgatgaga tattaaaggc taggtataat ggtgttgaaa taatggttga cacagtacca
     6001 agaagttctg ggcattgtat gaagaaaaaa agagtaattc aatttattat ggcaatatca
     6061 tctagtctat ttgaattagg tatagatggg gtaatggatg ctcttagaaa tgaagaggga
     6121 agaacactta tattaaaaga agcatttata cttggagata gggggagtat aatacttcta
     6181 aacactaaag atattaatat ggaaaagaaa tcaatacgtg aaatagctac acaaaaagga
     6241 atagatgaag acaagttatt actcgattta cttttagatg gagacgaaga acttatcttt
     6301 tgtttaggag gaatgtatag agctgatttc ccagataaac tacatgatga taaaataatt
     6361 gataatccat ttgttatggt tggttcagat tgtttgttta gtgtgggtgg ggatatgtca
     6421 tggtttgaac tacaaagaag aggagctttt ccaattttct tcaatatgta cagaaagtct
     6481 ggtgttagat tggaagagat tgtaagaaga gttacttcat taccagctag gcaatttaaa
     6541 ataaaaaata gaggtattct taaagaaggg ttgatagctg atatagcagt tattgacatg
     6601 gataactata gttatcctag gtcagaagat attgattttt caaagccaca attactagcc
     6661 aatggtgttg aatatgtaat tgtaaatgga aaaattgcat tagaaaatgg agagattact
     6721 gagaataaat gtggtgaagt attgaaaaga taaggttcta aaaataatag gatttagaaa
     6781 gtaaataaat atctaagtaa gtaaagacga agtttgagat ttatttaaat aaaaattatg
     6841 cttcgttttt atttatagta ttaatattta ttttaatact cagataatat ttatacaaaa
     6901 aatatttata ctttatagtg aaaaaattaa tatttttaat cattaaatta tgtaaaaaat
     6961 tggataaata gataactaaa tcatttgaga aaagattttt agaaataaaa aatgataact
     7021 aaataacccc tttaatattt
//
LOCUS       DAGSHT010000175         7024 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGSHT010000175 DAGSHT010000000
VERSION     DAGSHT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.181"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            318..1307
                     /locus_tag="MJB72_002993"
     CDS             318..1307
                     /locus_tag="MJB72_002993"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBY8264068.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1379..2119
                     /locus_tag="MJB72_002994"
     CDS             1379..2119
                     /locus_tag="MJB72_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBY8264069.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2319..3470
                     /locus_tag="MJB72_002995"
     CDS             2319..3470
                     /locus_tag="MJB72_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBY8264070.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3495..4250
                     /locus_tag="MJB72_002996"
     CDS             3495..4250
                     /locus_tag="MJB72_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBY8264071.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4442..6829
                     /locus_tag="MJB72_002997"
     CDS             4442..6829
                     /locus_tag="MJB72_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBY8264072.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         2848 a          715 c         1290 g         2171 t
ORIGIN      
        1 aaaagaagaa tatagatagt gtcaaggttt gcactatatc aaaacttaag gtaatcaaaa
       61 taagtcctcc tttgtaattc aaagttttaa ttatataact taaattctta tgtattgaaa
      121 ttttattatc aaatgtatat taataaatgt atattaaaaa gaatcagtag atataattag
      181 atagtcataa attaaattaa aatattttaa tgagatatga cttttttaga attaaaaacc
      241 gtagtatatt gatataatag aaataagact taaaaatctg ttgatagtat tatttataag
      301 tacagtagga ggataaaatg tttgaaaatt tacctaagat agatttacat tgccacttag
      361 atggaagtgt tagagtagaa accatgcttg atatagctat taaagagaaa atagatttac
      421 cttcaaataa tatggatgaa ataaaaaaat tagcaaaggt ttcttttaat tgtacttcac
      481 tagatgaata tttagaaaaa tttgatttac ccttaaaagt aatgcaatct aaggaaaatt
      541 taaaaagaat tacttttgaa cttttggaag atgcaagtag agaaaatgta aaatatattg
      601 aaattagatt tgcaccatta cttcacaccc aaaaaggtat gagtgttaaa aatataattg
      661 agggtataat tgaaggaatt agagaagctg aaagtattta tgatataaaa ggcaatttaa
      721 tattaggttg tatgagaaca atgacctcaa aagaggcatt attagtaatt gaagaaggga
      781 aatcatttgt aaataaaggt gttgtagcag tagacttgtg tggtccagag aaagagggat
      841 tttgtaagga atataaagat gtttttaaat tagcaagaga atatggatat aaagttacaa
      901 tacatgcagg agaagctgca agtggagaaa atgtattgga tgctataaat attttaaagg
      961 cggataggat tggtcatggg gtaaaaatta aagaccataa aaaggcgtat aatttagtta
     1021 aagataaaaa aatattgctt gaattatgtc caactagtaa tgtacaaact aagacagtag
     1081 attcttatga agcacatcca ttttatactt tttataaaga taatttacat gtaagtataa
     1141 atactgataa tagaactgta tcagatataa atttaagttc agaactaaat gttatatttg
     1201 atacttttaa actaggatta gaagactata aaataattta tagaaatgca gtagaagcta
     1261 gttttgcaga taaagaaact aaagagtatt tgaatagttt aatttaatag ataaaaatat
     1321 ttaaacttta aattaagcta ttaaattgat aaaaatacat tgtattagga gaaaaaatgt
     1381 gaaagattat gaagatgttg tttatattaa actagtcaaa aaagaacttg aagaatggaa
     1441 gaaaaatata ttaaagaaac cttctatatt tgacaaagca tcaaagggta tacagaataa
     1501 atttaatgga gtattgccag ataaatatca tgaaataatt acttcttcta taaaagtaat
     1561 gacaaaaacc gtactgtttg gatataaata tgctacaaag ccaccactta gaaatatgtc
     1621 attagaagaa agagaaaaat tagttttaga aaaagctaaa acttacaaga ctacagcaat
     1681 gattgaaggg gcaggaacag gggcaggagg aatcctctta ggtttatctg attttccttt
     1741 gcttttaggt attaaagtga aatttttata tgatgtagct tcaatatatg gatatgatgt
     1801 taaagattat agagaaagga tttatatatt aaatatatta cagttggctt tttctagtca
     1861 acagcatata aacaaaactt ttaaattgat gaataactgg gatgaatatg taaatagtct
     1921 accagaagac atcaatgatt ttgattggag aacatttcaa caagagtata gggattattt
     1981 agatttagct aagtttttac aactaatgcc tggaatagga gcatttgtag gattttatgt
     2041 gaatggaaaa ttaattgata aattatgtga aactgctgtg tctgcttata gattgaggat
     2101 tcgagagctt aagttataag agaaattata acataaaaaa ttagttatac aatatatcta
     2161 tatgtatcta attttgcatt gataaaaaca attatggata aaaggtaaag catgatagaa
     2221 tctaatgtag aggtttgtat tttaatggaa aaatttagtt tttattgctt aagatacaaa
     2281 ttgaattacg aagagaagta attgggagga atattttcat gacaaatttg ccaaaacaat
     2341 ttgatagctt caatgaagct agacaaaaag gatttataaa tgcaaaggaa cttaaggaaa
     2401 gtgggaaaaa gatggttgga gttttctgta catttactcc agttgaaatt ccaatggcag
     2461 ctggagcaac tgtagtaggt gtatgcggcg ttagtgaaga gccaatacct gatgcagaaa
     2521 gggtgcttcc aagaaatctt tgtcctttaa ttaaatcaag ctatggacat gcaataacag
     2581 atacctgtcc ttatttctat ttttctgatt tgttgatagg agaaaccact tgtgatggta
     2641 agaaaaaaat gtatgaagaa cttgcaaaag ttaagccaac atatgtaatg catttgccaa
     2701 atacttcaaa aggagaattt gcctataaac tttggaaaga tgaaatgata agattaaaag
     2761 aagaggttga gaaatcttta ggtgtaacta taactgaaga agatataaga actgctataa
     2821 aagataaaaa tgaagaaaga gaacttttaa aagagtttta tgcacttggt aaattacaac
     2881 catctgcact tacaggcctt gagttacata atgttttata tcaagcaggt tttaaatttg
     2941 accgtgcaga acttaaacat tctttaagaa aagttataga tgatatgaaa gaaagatatg
     3001 aaaaaggtga atgtcctgtt caaaaagata agccaagaat attgataaca ggttctccta
     3061 ttggtggaat aagcgaaaaa atagttaaaa ctttagaaga tgcaggggca tcagtcgttg
     3121 catatgaact ttgtggagct attagatcaa acgatttatt ggttgatgaa gaaatagaag
     3181 atgtatatga tgctttaaca caaaaatata taaatatagg ttgttcttgt atgatgaata
     3241 atgacaatcg tattgaatta ttagatagaa taatagatga atataatgta gatgctgtaa
     3301 tagatgtagt tttacaagca tgtcatacat ttaatataga aagttataga ataagagaat
     3361 ttgttacaaa agaaaaaaat aagccattta tgtctcttga aactgattat tcaaaatcag
     3421 atactgaaca attaagaact agatttgaag cgtttgtaga gatgttgtag gaataaataa
     3481 aagtaggagg aactatgttt agtataggag ttgactcagg gtcagtggca acaaagggtg
     3541 tgctatttga tggtgaaaaa attattaaga aaataattat accaactgga tggagcccta
     3601 aaagtacatc taaacaggtc tatgagttgc taagtagtga aattgacaaa aaagatataa
     3661 aaaaggtggt aggtacaggt tatggaaggg gagtaatgga ttttgctgat aagaaagtaa
     3721 cagaaataac ttgtcatact agaggtattt actttttaaa caagaacata aggactattt
     3781 tagatgtagg tggtcaagat agtaaagtta taaatttaga tagagatgga aatgtattta
     3841 actttataat gaatgataag tgtgctgcag gaactggtcg atttttagag attacttcta
     3901 accttttagg aagcgatatt gaaagtatag atacattagc taaaggatat gaaccagtta
     3961 atatatctag tatgtgtaca gtttttgctg agtctgaaat tgtaagtctt ctagctcaaa
     4021 acatttctac tggagaggta gcagcaggga tacttaaatc tattgcaaat aaatccacct
     4081 caatgttagc aagaggtgag gtaatagatg aagttgcatt tactggaggg cttgcaaaaa
     4141 gtaaagaact agtaaaaatg atagaagaaa tactgggtaa aaaaattttt attgcagaag
     4201 atactcaaat aataggagct ttaggagctg ctgtaatagg atttagataa aaacatataa
     4261 aaggaaaatg agaagctgaa tttattataa ataaattcag ttttttattt tattttattt
     4321 atttataaag gaaaactttg gtataattga tttaaatgat tgtatatttg taaatacttg
     4381 atatttagtc atatataaca ttataatttt aaaaattagc tcaaaataag ggggagaaag
     4441 cgtgaaaaaa tttacttcaa aaaaagtaac aaggtttgtt gcgctgtttt taatatctat
     4501 tttagtaatg tcaacaattc ctgtatcagc tgacaatagc aatacattga atcagataaa
     4561 aaaagcagaa tacagtgaga cgtataaaca atatctaaat gatgctaaaa atggacgtac
     4621 agaaaaatat aatgggatta taccaaatcc atatcagttg gaaggtactc aaatccaaag
     4681 taaaggaaga acagctccaa catcttatga ccctagaaaa ttaggtctta tgacatcaat
     4741 aaaaaatcaa gaggatttag gaatctgttg ggattttgct gcaatggcaa ccttagaaag
     4801 tttcttaaag ttaaataatt atggagacta tgatttatca gaagaacact taagatggtg
     4861 ggcttcagat ggtgaatatg gatggtctgt aaatgatatg aatggtgctt taaattatga
     4921 agctatggga tatttaacat cttggtcagg tcctaaacta gaaaaagata ttccatacaa
     4981 tggtagagta tctaaagcac aaggagcaaa aaaaccaact aatatgaata cagcacctac
     5041 tgtttttaat gtgacagatg ctgtgtgtgt aagtaatgac ataaattcta caaaaaatgc
     5101 tatattacag tatggagcag taacatctgg ttattatgaa gatattaaat atcaaagtga
     5161 tgaccaaaat tcatattatt gtccaacaaa aagttttaat acaaatcatg ctatttcaat
     5221 tgtaggttgg gatgataact attctaaaga taattttaat gcaaatatta gaccatcaaa
     5281 aaatggtgct tggttaataa aaaatagctg gggagattat aattctgagg gtggatattt
     5341 ctggatatct tatgaagaca agacacttat gtctgatata gataattttt caatcaaagg
     5401 tggtaaaaaa ccaaatgatg atgagaagat gtatcaacat gactatgcaa gtattgtacc
     5461 tttaatttca aaaaagataa cagctgcaaa tgtatttgat ttcaatagag gcgatgaaac
     5521 tctaaaatct gtaatgttct taactgaaag tataggagct aagtatgaag tatattatgc
     5581 tccagtagta aatggtatac ctcaagaaaa caatatgaaa aaattaaaag aaggtactgc
     5641 ccaatattca ggatacataa cagttccaat tgattctttt gatataccag aaggaaaggg
     5701 tgcaattgta gtcagtatag aggggaaaaa tggagagtca actataggtt ctgagtcaaa
     5761 tgtaccagga tatgatacat ttaaagcaaa agctaattta ggtgaaagtt atatcataga
     5821 taatactgga aaattttttg atataaatag agatagtaac ttctatccgt gtaattttac
     5881 tataaaagct gttacagaaa agtcttctgg tgagagtatc ccaaatgagt ctttaattgg
     5941 tagtgataga tatgaaactg ctatcaaagt tagtcaaaat ggtttcaatt cttcagaaaa
     6001 tgtagtttta gtaaatggca gttcaatagt agatgcattg gcagcaactc cttttacatc
     6061 tgctataaat tcaccaatat tattgacaca aaaagaagct ttaaattcaa aaactaaagc
     6121 tgagatacag agattaggtg ctaagaaagt atacctaata ggtggagaaa attctataag
     6181 taaagaaatt gaacaacaat taaaaagctt aaatatatcg atagagagaa tatcaggaag
     6241 tgatagatac aagacaagtt tattattagc tcaaaagtta aatggtataa aaaatgtatc
     6301 tcaaatagca gttgtaaatg gagtgaaagg attagctgat gcaattagtg taggcgctgc
     6361 agcagcagaa aataacatac caatcatact tgcaaatgaa aagagcgaat tgcaaggagc
     6421 tgatgaattt ctaaattcat taaatataga gaaatcttat attattggag gaacagcatc
     6481 cctatcaaac aatctagaaa gtaagttaaa aaatccaact agattatctg gtagtagtag
     6541 agacgaaact aattcaaaga taatagataa tttttataaa aaagatactt taaaaaatgc
     6601 atttgtagtt aaaaatggaa tcaagaatca aaatgattta atagatggat tggcagttgg
     6661 tcctttagga gcaaaaactg ggtcaccagt aattttagtt ggtgataaat tagctgatag
     6721 ccaaaaagaa gttcttaaaa ataagacttt agaaaaagta actcaagttg gtggaggagc
     6781 aaataaaaat gcatttaaag agttagtaaa attaaaaagt gctaaataga gtacttaagt
     6841 attaacatat attagtgtgt attagcaact tatcttaata aaagctgaaa ataattactt
     6901 taaattattg ataaataaga taatcaatga ttgtaaagtt ttgttttcag ctttaaattt
     6961 atgtagtata agtttaaaaa cataaaaaag ggttacttta tatagaaata tagaaaatct
     7021 taca
//
LOCUS       DAGSHT010000176         6929 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGSHT010000176 DAGSHT010000000
VERSION     DAGSHT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.182"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..746)
                     /locus_tag="MJB72_002998"
     CDS             complement(<1..746)
                     /locus_tag="MJB72_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264073.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTI"
     gene            complement(746..1492)
                     /locus_tag="MJB72_002999"
     CDS             complement(746..1492)
                     /locus_tag="MJB72_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264074.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(1639..3858)
                     /locus_tag="MJB72_003000"
     CDS             complement(1639..3858)
                     /locus_tag="MJB72_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264075.1"
                     /translation="MKKRENVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     SEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIASNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEACKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            complement(4224..5618)
                     /gene="gltA"
                     /locus_tag="MJB72_003001"
     CDS             complement(4224..5618)
                     /gene="gltA"
                     /locus_tag="MJB72_003001"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBY8264076.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            complement(5618..6511)
                     /locus_tag="MJB72_003002"
     CDS             complement(5618..6511)
                     /locus_tag="MJB72_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBY8264077.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
BASE COUNT         2302 a         1258 c          663 g         2706 t
ORIGIN      
        1 aaaatagtaa attgagaaaa aaataaaaat atccccaaag taattaatag tgctgttgta
       61 tacatacctc catttatttt taacttattg ccaaatagta tcatagcata tcctaatata
      121 agacatatta caccaattat tccaaatagc tttgaagatt ttatctcttt tttaggcttt
      181 tttgtaccta ctaaaagctc taaaatactc tttttactca ataattttaa gctcattggt
      241 cctgttatta aaaataaaat cataaatgat acaatagtca atagcattgc ttttactgga
      301 aaataaaatt cagttaaatt catataaaac agtttgctta aatacaataa aaacaatttt
      361 gaaattgata aaccaagtaa tattcctaac atcactgcta aaaatcctat tattaaattt
      421 tcaattaata tcaatttttt cagttgtctg ttgctcattc caattatcat caatactcca
      481 aaatctttca atctatattt caagaaattt cctaaagagt acaatataaa aagtagacta
      541 aaacctaata ttattaattc tgaagtcata agagccataa atgcagtaga accactctgc
      601 attccagatt ttgttataat tggatggaac atagacactg caaaaacaaa aaatatcatt
      661 atagaaaaaa cagaacttaa aaaatatgct aaatatgccc tgaaatttct aactatatta
      721 ttataagcaa actttctaaa atccactatc ttacacctcc tatttgagac aaaacagcta
      781 gtatatcaga atagaaagaa ttactgcttt cagatttttt tatttctgta tatatacaac
      841 catcttttat aaaaattact ctatctgaat aacttgcact atatgcttca tgagttacca
      901 taagtgttgt taccttttgt tctttgttta tcctagtaaa taatttcaaa acatcatcag
      961 tagattttga atcaagattt ccagttggct catctgcaag aagtatggca ggtttattta
     1021 taatagctct agcaatagca cacctttgag cttgacctcc tgaaatttca taggtttttc
     1081 tatctaaaat tttatctatt cctaaaaatt tagatatact tttagtttgt atattcatat
     1141 ctttgatact tgccccctct agtgtaagtg gcaacataat attttcgcca acagttaaag
     1201 tgtcaatcaa attaaaatcc tgaaatacaa aaccaagttg tttccttcta aattcagcaa
     1261 gtttttctcc tttcaattta cttacatcat atccatctaa tacaacctca ccttcactct
     1321 gtctatcaac tgtagatatt atatttaaaa gagtagattt accacttcca gatggtccca
     1381 taacagacac aaattctccc ttttctatat ttagactaac cttatctaat gctttaaatg
     1441 gaatatttcc acaatatgtt tttgatatat ttctaatact caatatgttc attttatatt
     1501 ctccataatt ttttattttt actacaatac cataatatcg tgaatatact actttaacca
     1561 taaaaatacc ctaacaatta acttacatat ttgtaagtaa gttgttaggg tatcttatag
     1621 tctaataatt attagtgatt aatcaaattt aaagtttaag tgtttttcaa attcatctaa
     1681 taccataggt ttagcaaata agtatccttg aaccttatca caacctatct ctttcaagaa
     1741 ctctacttgt tcataagttt ctacgccctc acagattact ttaatatcaa tcgcttttgc
     1801 catttccaca atcttagaaa taataatttt tccacgtttt gtatctgttg tttcgattaa
     1861 aaatcctcta tctaatttta aaatatcaaa agaaaggtct tttaacagat ttaaagatga
     1921 atatcccgaa ccaaaatcat ccattgatat aaggaatcct atctctttaa tctttttcat
     1981 tatatcaatc aatatattta aattatcaaa cacaacactt tctgttagtt caagttcaat
     2041 aaactcagga gatatattgt atttacttat caaatcagtt atattttcta taaaattatc
     2101 cctatataaa tgaactctag acatatttac agagattggt acaggagtat accctttatt
     2161 tatccacttg tttaatgttt tgcatgcttc ttcaaacacg aacatatcaa tatttacaat
     2221 aaaaccattc ttttcaaata aaggtacaaa atctataggt gaaataaacc caaattctgg
     2281 actattccat cttatcaagg cttctgctcc ttcaatttca tttacatcat ctaaactata
     2341 cttaggttga taatatacta taaattcacc tttttctaaa gctttattca tattatcttc
     2401 caatcgtttt tccttgtaca atcgatttct tgtatcttca ttaaaaaatg aatatgaatg
     2461 ttcatatttt tcctttttac tttttgctgc aatatttgct ctatcaatta acaagtcaat
     2521 ctttttaata tcatcctttt tagtaatctt atatattcca cttgacataa caattttata
     2581 cttagatgag ttagtagtat ttaagttaca aatttcttta tttattttag ataatctatt
     2641 taaaagttca tcttcagtgt tagaagcaat caacaatata aaatggtcat tatccatcct
     2701 agcacaaatt tcatatttat ttgtattatt atttaaaact tgtgatatat gaattaatat
     2761 cttattccct tcagcatatc caaacatctc atttatatat ttaaatttat tgacatccaa
     2821 ttgtactaag taaaaattat cttgataata attctttaaa atatacatag aatcttttct
     2881 aaacttttct ttatttttta ttccacttaa agaatcataa taagccaatt ttttaagttg
     2941 ttctattaat ttagcattac ttaatttcca caatatcata aatattacta tataaaaata
     3001 caactttatc ataaaattaa ctattagatt tttaaagttc ttaaatatat tacattttga
     3061 tactaaagtc caattagtcc actttatgtt cgtatatttt acattatata atctccaatt
     3121 atcccaagat aaaaaatcac cttgacattt tttatttata ttttttaaga tttcactttt
     3181 agtggttcct ataatattcc cttctaaatc aagcatctta cttttatcat ttacatattt
     3241 tttgtttgga tgagaaataa tagtataatc tttatctatc attagaaatt taatagaatc
     3301 atcaaaattg cttctactta ttaaattatt tacatcttga aaataaaaag aagcaaaaat
     3361 ggtcccaatc atctcactat tttttacaat tggtacatat atgataaata ctttgtcacc
     3421 tgttacatta ctgacttcaa tatctgaaac tactacttgc tttgttttaa ttgctttctc
     3481 aaaactaggc ttatctttta ttgagttaac attttcccta tatgtacttg aagcatttcc
     3541 ttttgtatct gttataccaa tcataaaaag attatattcc ttttgatatg gacttaataa
     3601 ttttcccttt tcaattagtg atgtttcacc attagaaaat ctatcatctt gactaagagc
     3661 ttttaataaa gatatgttta cttctaaata gttttcaagt tctctttttg atgcctcaat
     3721 catatttcta gaagtaattt ccatagagtt acttgcatat atgatggtac ttgttaaatc
     3781 aatcaaaact aatagtatta ataagattaa tatatgctta aaaactagcc catcaaatac
     3841 gttttctctt tttttcatat taaaatacca ttaattacac aatttatttt tctattattt
     3901 aatgttagat ttgacaaatc tgattttatt agccacacat tattcactcc ttacaagacc
     3961 tttattgtat aactatttta tcatagttag acaaagttat gttacttagt atcaaatatt
     4021 acaataataa ctgataaatt attaaaccat ctaaaaaagt ggctacatca aaatagagat
     4081 tgtactaaga cctaaaagtt agactttcta caagaatgaa attttcattg ttgaaatgat
     4141 taaaacatat ttgattttgt acaaaaagag tgcctcatga actaaaaagt tcattttgag
     4201 acaccctctc tttaatttac aaattaagca ttaacttttg cttttaaata ttcatctatt
     4261 gatgctgctg ctgttttacc agcacccatt gcagatatta ctgttgctgc tccagtaaca
     4321 gcatctccac ctgcaaatac accctcttta gaagtctgac cattttcatc tgttattaag
     4381 catctcttct tatttatatc aagactcttt gttgtagaag atattagtgg atttgggctt
     4441 gttccaagtg acattataac tgtgtctaca tcaagtacaa attcagaacc ttctatttct
     4501 ataggtcttc ttcttccaga atcatctggc tctcctaatt ccattcttat acaaaccata
     4561 cctttaacat atccattttc atctactaaa atttccttag gatttgttaa tgtattaaat
     4621 ataatgcctt cttcttttgc atgatgaact tcttcagctc ttgctggaag ctctttttca
     4681 cttcttctat aaactatata tgattctgaa cctaatctta atgcagttct agcagcgtcc
     4741 atagcaacat ttccaccacc tactacagca acttttttac cagaactaat aggtgtatca
     4801 taatcatctc tataagcctt cattaagttt actctagtta aaaactcatt tgcagaaaat
     4861 actccatttg cattttcccc tggtatattc ataaacattg gaagaccagc tcctgaacct
     4921 atgaatatag cttcaaatcc ttcatcttct ataagttcat ctactgtaat tgttttacca
     4981 acaattacat tagtttctat ttttacacct aattttctta tattgtctat ttcaggttga
     5041 acaacagctt gttttggaag tctaaactca ggtattccgt aagttaaaac tccacctggc
     5101 tcatgcattg cttcaaagat tgttacatca tagccttttt tagctaaatc accagcacaa
     5161 gctagacctg ctggaccact tcctattact gctacttttt gatttttctt aggctctgta
     5221 tcagataaat ttatgtcatt ttcttttgac caatctgcta caaatctttc taatttacct
     5281 attgatacag catctgattt tattccaagt atacatacac cttcacattg actttcttgt
     5341 gggcatactc taccacaaac agctggtaat gaactgctct tagctattac tttagcagca
     5401 ccttctatat catcttcttt aatttttgtt ataaaccctg gtatgtcgat tcctactgga
     5461 catccaccca cacattttgg ttttttacaa gctaaacatc tattagcttc tgccatagct
     5521 tcttctttat tataccctaa gcatacttca tcaaaatttg ttgctctcac agcaggttct
     5581 tgttctctaa ctggtacttt tactttttta gcatccatta tttgttacct ccacatccac
     5641 aattactatg atgatgacta tcgccttctt caagttttag catagctctt ccctcttgag
     5701 tcttgtacat agactgtctt ctcattgctt gatcaaaatc aactaaatga ccatcaaatt
     5761 caggaccatc tacacatgca aacttagttt catttccaac actaactcta caaccaccac
     5821 acatacctgt tccatctatc attattgtat ttaaacttac tatagtaggt atatttagtt
     5881 ctttagttaa ttggcacata aatttcatca ttatcattgg tccaattact attgcttggt
     5941 cgtatttttt accttgatta tcaactaaat cttttaatag gtctgtaact cttccattaa
     6001 atccatatgt accatcatct gttgatacat atacattttt agctactttt ttcatatcat
     6061 cttctaatat aattaattct ttagttcttg caccaattat tacatctaca tctaatccat
     6121 gctctttaaa ccatttaact tgtggataaa ctggagcaga accaactcca ccagctacaa
     6181 ataatatctt tttatttctt aattcttcta tatcttcatg tataaattca cttgcttgtc
     6241 ctaatggccc tacaaaatcc attacaaaat cgttttcttc aaacattgct aactttttag
     6301 ttgaagcacc aactgtttgg aatacaatag ttactgttcc tttttctcta tcatagtcag
     6361 ctattgtaag aggaatcctc tctccttttt catcattttt aattattata aattgacctg
     6421 gttgagaaga ttttgcaact ctaggtgcct ctatttccat taaataaata ctatctgtta
     6481 attgtctctt actaactatt ttattactca tctcgctatt ctccattcta acaaatatat
     6541 ttttcccact ttaaatttaa ttaaaaacat aaacacacaa aactataact ctttataata
     6601 taatattagt tcttggttga ttgttttgta ttggacatcc cccctataat aattataact
     6661 gcccaaacta caaatagttg atattaacaa atttcttgta aatgccaata aacactaagt
     6721 ctatatcata cgtctaattt gttatagatt taacctaata cacacttaaa gtatactata
     6781 ttatgccaaa tttgtaacaa aaattttata aatacataat aaaatttttt tattcaatta
     6841 tttatttaat aaattaactt aggttttcat aataacacta atttctttaa aaactaaacg
     6901 tgtataaggc tttaatctat tgcaatacc
//
LOCUS       DAGSHT010000177         6881 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGSHT010000177 DAGSHT010000000
VERSION     DAGSHT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.183"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(152..991)
                     /locus_tag="MJB72_003003"
     CDS             complement(152..991)
                     /locus_tag="MJB72_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY8264078.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1015..1773)
                     /locus_tag="MJB72_003004"
     CDS             complement(1015..1773)
                     /locus_tag="MJB72_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8264079.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1820..2293)
                     /locus_tag="MJB72_003005"
     CDS             complement(1820..2293)
                     /locus_tag="MJB72_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8264080.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2322..2738)
                     /locus_tag="MJB72_003006"
     CDS             complement(2322..2738)
                     /locus_tag="MJB72_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8264081.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2785..3837)
                     /locus_tag="MJB72_003007"
     CDS             complement(2785..3837)
                     /locus_tag="MJB72_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY8264082.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4233..4913
                     /locus_tag="MJB72_003008"
     CDS             4233..4913
                     /locus_tag="MJB72_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBY8264083.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5046..5366)
                     /gene="sugE"
                     /locus_tag="MJB72_003009"
     CDS             complement(5046..5366)
                     /gene="sugE"
                     /locus_tag="MJB72_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBY8264084.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5748..6575
                     /locus_tag="MJB72_003010"
     CDS             5748..6575
                     /locus_tag="MJB72_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBY8264085.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2317 a         1259 c          819 g         2486 t
ORIGIN      
        1 atattaaata ctacatatag cttacataag tttaaaattt aatctattat aaaattaccc
       61 taattgccaa actttttaat ataaattttc caacatccta caaatataat caatagatat
      121 agaatcctaa cttacaccaa tatatattta tttaaaatag tcctaaaata cctgtaatag
      181 ctcccactac tatcagtata aacattattt ttattggaga aagacctttt tttaatgcaa
      241 agagtactaa taaagtaagt accaaaggaa ttaatcctgg aaatatctta tcaagtaaat
      301 ccgtttgaag actaaattta aaatcaccac tagtataact tattggtgta gataaattga
      361 taaacctacc tacaagcccc cccatgacta aggtacctag tatagaagcc ccttctataa
      421 ccttatttac tattccacct tgaagtatac tttcaacagc ttttttccca tacttatatc
      481 catttgtcca cattccaaaa gaaattgaag tcattattat atattgagct attacaaata
      541 atataggtcc tgcaattgat gctcctccct cagttaaacc aatacaaaca gctaacacta
      601 taggagttat aactccttga gttaaagtat caccaattcc cgctagtgga cccatcaagc
      661 ctgttttgat agcattcata ccatctccac ttatttcatc tgctccatta gctttttctt
      721 cttccatagc tattgttatt ccatgtataa cacatccaca tataggttct gtgttaaaaa
      781 atgctagatg agtctccact tcttgtttta actcttcctt atctggatat aattttttaa
      841 gtactggcaa cattgaatgt gaaaaagctg ttgattgcaa tctttcataa ttgtaattag
      901 attgtgcaaa gaaaaaccat cttaaccatg attttattac atctttcttg cttaacgttt
      961 tcttagatac ttcatttttt atatctgaca taatattttc cccctcacat gtatttaaga
     1021 tgtagcacta tcttttccgt acttaattga agtaattgta agagcaagta ttactccaat
     1081 aacagatatt actattatat ctactttaaa ataaacagcc attagtattc ctaatatgaa
     1141 aaagggcata gtaaacttgt ttccaatcaa ttttaaattc atagcaatcc caagtgctgg
     1201 caacatagcc ccaattacat taaatacatg taacacatta tcattcatta cttccaacat
     1261 accatctatc gcatttggac caaaataaac aactaataat gctggtataa aactcattgc
     1321 aaataacagt atctgtggtg gtactatatt tataaatgct actctatcaa cattcccttt
     1381 ttgaacctct ctatcagccc aatgtgcaaa aaaagaattt actgtcatct ttccaatcca
     1441 aagtacagtt gcaacagttc ccaatggtac agctattgcc attgcagtac ttacatctag
     1501 cttagatgct atagctaaag cagtacctac atatcctgca aaagctgggt cccccatctg
     1561 agctccccct gctgatataa aacctagata tatcatattt atacctgctc ctataattgc
     1621 tccttgaaca gggtctccta atataattcc tgttacaaat ccagccaata gaggtctatt
     1681 ccatgtaaaa aatccaacat aagtcaacca cggactccaa gaaagataat aaaaaattgc
     1741 tattaatact gcttgaatta gtgaaatgtg catattcttc ctccctattt tcttttaaag
     1801 atttacaaga tagtcttctt tacaataatg ttcctacatc aattttcttt gcatctggta
     1861 ctatttgtat aaagcattta acatcttttg atattagctc tctaaatatt tctttttctt
     1921 catctgacac agatatattt ttataaaaac tttttctacc aagccctgct cccatacctc
     1981 caacattcaa ttcctttaaa tctaccccgc tatttactaa acctaagaca gttttagggt
     2041 atttgaccaa tactattaaa ttttcatctc cattatgtga ttttaagact tcagcacctg
     2101 attctacatc atgtacttca accttaacag ttgatggagc tgccatttgt aaaacttgaa
     2161 ctataaatgg gtcctttact actaaatcat ccacgattac tatccttttt gctgatgtaa
     2221 tcttacacca agcagtaata acttgcccat gtattaatct atcatcaatt cttgttaatg
     2281 ctatattact cataattatt cctccagatt tttcatttat tttacaactt taaaacagtt
     2341 ttaacatctt tgattcctga tatcccaact tcctcacata aattcttcaa gtcttttaac
     2401 ttcatagtat ctctgtttaa aaataactct aaaagcatag gtaaatttac acctgtcaaa
     2461 cattctaact cacatacctt agcaaattta tttattataa tagcagatgt attaaatgga
     2521 gttccgccat aaaaatcaag caatactaaa accccttcat ccttatattt ttcaattcca
     2581 tcttctaact ttctactaaa ctcatctatg ctgtcaccat ggtttaatcc taaagtttct
     2641 acattttcct gtttgcctac tattaattca gcactttcta acattgcttt agccatattt
     2701 ccatgtgtgg ctataattat tcctgccata tgaatcatac ctttcttaac cttttttata
     2761 tggattatta tttagtactc taatctaatt atctatatta atcattatct taccaaagaa
     2821 aatatccctt ttgtatactt tttcaaaata agctggtact tcttctaaat ttattctatg
     2881 tgttactaat ttttcaacat tcatatcacc cttttgcata tgatacaatc cagcactcca
     2941 ttcttttcct gggaatggaa aggagtttcc aaaccaagtt ccctttacag ttaattcact
     3001 tcttactatc ttttcaaatt gctctctagt caaagctaca tccccatatg gtacacctgc
     3061 atacaatact gtacctccct ttttagctag caacagtact tgaccacatg tcagtggagt
     3121 accagccgat tcaataacaa tatctgctcc atctccatca gttaaccttt ttatttcttc
     3181 tacaatattc ttttcttttg catttataca aatatctgcc cctaactctt ttgccaagtc
     3241 taacttctca tcaaatacat ccacagctat tacttttgta gaaccaaata tttttgccca
     3301 ttgtattgaa aataatccta ttggacctgt accaagtact acaactgtat ctccaacttt
     3361 agcttctgac ctaaaaagac catgtcctgc tatacatact ggttctagtg cagcggcagt
     3421 ctcataactt atctcatctg gtatttttat taaatttctc tctttcacct ttgtatactc
     3481 tgcaaaacaa cctccaagtt ctttatttcc tattattgct acattattac atcttgaata
     3541 caagcctttt ttacattcat cacattcaaa acatggcata gctggacaaa cagcaactct
     3601 atctcctatt ttaaaactcc ttacttcttt accaacctgt gccacttctc cagaaaattc
     3661 atgccctaaa atttctccaa ccatatgtgg acctgtcttt gaatattttg atatatcaga
     3721 accacatatt ccagctactt ttactttaat tattacatca tctttttcta atatttttgg
     3781 tacatccaca tcctccactc ttgtgtctct tataccatag aatctaactg acttcactat
     3841 taaccacccc tactttttat ttgagaatat atccctattg tttatatgtt aaatatctaa
     3901 agcaactttc atgccaatat atgtaagcat tgtattcatg cattttataa aactatcatt
     3961 aaatgtgtgt caaagtgtgt caaaagtttc ttatattaaa acttatgtgt gtcaatataa
     4021 acgacactaa cattttatat tagttctctt attataagtt ttataaaatg tacttttttt
     4081 attattttta taaaattgtt cggaaatttc ccttctattt gtgatataat attaaatata
     4141 aggtatcaat ataaattttc atgcgaatat cagaactttt ttgacactaa tgccttattc
     4201 aatataataa tttaactttt tggaggttgt ttatgaaaaa agtgtatcaa ggaaagacta
     4261 aagatgtttt tgaattagat aatggaaatt acttacttaa atttaaagac gatgtaactg
     4321 gtaaagatgg agtatttgac ccaggcgaaa atagtgtagg tctaagtata gatggaattg
     4381 gtagagctaa tttagagaca tctgttaagt tctttgaaat tctaaacaat gctggaatta
     4441 aaactcatta tgtaagtgct aatttagaag aagcaactat ggaagttcta ccagcaaaag
     4501 tttttggtaa tggattagaa gttatttgtc gttatagagc tgttggtagt ttcttacgtc
     4561 gttatggttc atgtgttgaa gaaggtgcta aactagattg ttacgtagaa gcaactttaa
     4621 aagatgatga ccgttgtgac cctcttataa catctgaagg tttagaagct ttaaacatta
     4681 tgactcaagc tcaatttgat tcaatgaaaa gcatgactca aaaaatttct aacatagttc
     4741 gtgatactat agctgaaaaa ggtcttgagc tttatgatat taaatttgaa tttggattct
     4801 acaatgatga agtaatctta atagatgaaa tagcatctgg aaatatgcgt gtttataaag
     4861 atggtgtaat agttgaccct atggatttaa ctgctctttt attagataaa tagtatatac
     4921 atataattta caaatataat aaacttataa ataagataag ctatgaagta aagacttttt
     4981 aaatgttgat tatttttaaa cattttctcg cctttaattt catagcttat ttaaattaat
     5041 atactctaat tagtagataa tagctttaac cctacaattc ccaccacaat aaaacctata
     5101 catgcaattc ttgatatact aataggctca tttaaaatag ccatgcctac aatagctgta
     5161 ccgacggtac caattccagt ccatattgca tatgaagttc caagtggaag atgttttagt
     5221 gctaatgaca aaaaataaaa acttacaatc attccaacaa cagtaagtat acttggagtt
     5281 aatttagaaa atccatgtga attttttagt tgaaaagccc aaaacacttc aaacaaacct
     5341 gcaacaaaca aaaataacca tttcataaat tcatccctct tttcaaatat gtctcttatt
     5401 tttccaataa aataacctga gaaatattat aaaatatctc ccaggttttt gtccttccgt
     5461 gttcacaaca aaaactatgt tgtgggtttt atctcggacc agaccaatca ttaataattg
     5521 cggaacccta taaaactcta ttaattattc tattttatct ataataatac tatcaacata
     5581 tataaaaaaa gtcaatatat gggttccatc tataataaaa ataactttta tatacctttt
     5641 aatattctta ttcacttctc tccataccca tttaggtcaa tctaagaata tctaattttt
     5701 atttaatatg ttataataac ataaaaatat tgaaaggagt aatttctatg aatttctata
     5761 aaattgcagt atgtcagatg attactacag aaaataaaat tgaaaatatc aatcatgctg
     5821 tagatatggt aacagaagct gccatcaatg gagctaaaat tgtagttttg ccagaaatgt
     5881 ttaactgccc atatgaaaat aaatattttc ctaaatttgc tgaagaatat ccaggtgaaa
     5941 caacaacaat attgagtaaa ttagctgaga aacatggaat ttatctagtt tctggtagta
     6001 ttcctgagtt agaatatgga aaaatctata atacttgcta tgtatttgat aaaaatggag
     6061 ctctaattgg aaaacatcgt aaaatgcact tatttgacat tgaagttaca ggaaaagtta
     6121 gttttaaaga atcagatact cttacagctg gaaatgatgt cactgtaata gataccgaat
     6181 atggaaaagt tggtatagct atttgctatg atattcgttt ccctgagtta tcacgtttaa
     6241 tggctcttaa aggtgctgaa atcgttatct tacctgctgc atttaatatg actactggtc
     6301 ccgcccattg ggagttatct ataagaatgc gtgctttaga taatcagata ttctatgttg
     6361 gtgctgcacc tgctagaaat atgaatgctt cttatatagc ttttggaaat tcaagaattt
     6421 cagacccttg gggaagaata attgctcaag ctgatgaaaa agaatgtatt atttatgctg
     6481 acattgatag agatttaatt cctgacataa gacaacagtt accacttctt aagcacagaa
     6541 gaactgattt atatgaactt aatactttga aataaaacag aacataaccc cataaatcat
     6601 aaattaagtg ttaaaaaaat ttatggggtt ctcattaatt tgatttaaaa ttaatttcat
     6661 aaaaataatg ctaatattaa tttaattaaa taatactaat aattactaat gattatgcta
     6721 attatcagca tttactaatt taatcactat tagttatttc ttttattaaa tatctttact
     6781 tttttactta ttatgtcttc tagctttaca tttggattat ggtatttctt tctattttta
     6841 gtcacatctt tctattgaat tacttgctta aaacaccaat g
//
LOCUS       DAGSHT010000178         6869 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGSHT010000178 DAGSHT010000000
VERSION     DAGSHT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.184"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1930)
                     /gene="recJ"
                     /locus_tag="MJB72_003011"
     CDS             complement(<1..1930)
                     /gene="recJ"
                     /locus_tag="MJB72_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBY8264086.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNIN"
     gene            complement(1943..3607)
                     /locus_tag="MJB72_003012"
     CDS             complement(1943..3607)
                     /locus_tag="MJB72_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBY8264087.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3905..4402)
                     /locus_tag="MJB72_003013"
     CDS             complement(3905..4402)
                     /locus_tag="MJB72_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBY8264088.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4431..5810)
                     /gene="scfB"
                     /locus_tag="MJB72_003014"
     CDS             complement(4431..5810)
                     /gene="scfB"
                     /locus_tag="MJB72_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBY8264089.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPQFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6115..6261)
                     /locus_tag="MJB72_003015"
     CDS             complement(6115..6261)
                     /locus_tag="MJB72_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBY8264090.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6291..6869)
                     /locus_tag="MJB72_003016"
     CDS             complement(6291..6869)
                     /locus_tag="MJB72_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBY8264091.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
BASE COUNT         2234 a         1257 c          647 g         2731 t
ORIGIN      
        1 aatttatgtt aaaatccaaa tctataagtg atatatctga cattgctcta taaaatccat
       61 ttatagaatt agatattatt agtgtatctt tttcaaaata atcaaatata tttatatctg
      121 tatttccatc aaataattca aaatcagaca cagatatatt atacaggctt tgagtatctt
      181 ttggtattat ttttgacatt aatttcaatg aaagtttgtc attacttgca cttttaggtc
      241 gagccaatcg tatatcttta agtaaaaatt gtactttcct atttcccata tagttatttt
      301 catctaattg gaatagtaca tctaccttgt ctcctacttt atacattgat tttaaatgac
      361 tcatattaaa tcctatacat tcatagcttt tctctttttc tatgcttaat ttcagatgtt
      421 gttgattctt acctataact ttcaaatctt ttaaaatata atttctaact ataaatctag
      481 gattaggatt atttaatcca aagggttcta acttatgtaa ttcttctaca agatttaaat
      541 ttattacatt ttcttgtaat tcaaactcaa catttacatt ctctatcata tcatcttcag
      601 ttaaattata atctgcaaat ttatttattt catttgctaa aatttctaca ttgtctgaat
      661 ccaaggataa ccctgctgct tgttcatgac caccaaactt agtcatcaag tctttgcact
      721 ttatgagagc ttcaaatata ttaaatcctt ttattgacct cgctgaacca gtagcctccc
      781 cattttctat acctagaaga attgttggtt tgtagtattt ttcagttaac tttgatgcta
      841 caattcctat tattccatgt tgccaaccct ctttagctaa aaccaacacc ttgtcatcat
      901 tgtatctgct attagacttt agcatttcct cagcttcatg atacatctta gcttctatca
      961 tctgtctttc attatttttt tcttccaata tactagctat ttctactgcc tcttcttgtg
     1021 attgagtagt aaatagctct actcctaagt aagaatatcc caatcgacct gaagcattta
     1081 tcctaggacc tattgcaaaa cctatatgag aagaacctat cttatcactt tctacaccac
     1141 aaacttttat aagctccctt aatccaatat ttttgccttc tttcattgat tttaaaccat
     1201 tttttacaat tattctattt tcatctataa gtggaacaat atcacaaata gttgctagtg
     1261 ttactatttc caaataatta tacatactag ttttaaattc ttcttttgga gtcaaggctt
     1321 gaatcatttt aaatgctaca ccacatccac atagtgaatc aaatggataa ttacaatctt
     1381 cttgttttgg atttattact gcaaaggctg atggaatttc actttgacat tcatgatggt
     1441 ctgtaattat aacatctatg cccaaatcat tcgctgtatt gacctcagac actgatgtaa
     1501 ttccacagtc aacacttata atcaagtcac atcccctaga gcttataagc ttaatagcat
     1561 cttcatttat accataccct tcttctaatc tattaggaat atagtaatca acattgtaac
     1621 ctatactctt aaaatataag cacaagatag atgtagatga tacaccatca acatcataat
     1681 ctccgtaaat ccaaattttt tcgttcttct caactgctaa ctttattcta tcaacagctt
     1741 tttgcatatc tttcattaaa aaagggtctc ttaaataatc taaagatgga ttcataaata
     1801 tctctgaatc tttttcagta catatccctc tattttttaa aatttggcag atttccgggg
     1861 aaatattaag ttttttgcta aattcacttt cctctatctt acctttatac ttcaatatcc
     1921 attttttcaa atttaattcc ccctaatctt ttttgtattt tgacctaata cttcttataa
     1981 ctaagcaaag gcctattata aaggatataa agaaacccgc tattgcagta aattttatcc
     2041 atatattatt ttctatattt ggagatgcaa ttataagaga agaacctact ataatagatg
     2101 ccaacactaa actcaaagat atttgagatg ttagctctat tatacaattt tctaacctcg
     2161 tcattttaac atcttcaatt tgcattttta tattatttct ttctatattt tttaaaattg
     2221 cttttaattg ctttggaata gtttttatgt ccagtaatat ttcttctaca ttttgtcttg
     2281 aacttaaaac aacattttta gggttaaatt tactcttata atgatgtttc ataaactctt
     2341 ttcccataga accaaaagaa aaatctgtgt ttaaagttct acttgttcct tctaatgtta
     2401 taactgtctt tgcaagagtt acaaattgtg caggcatgac tattttatac tgtctaaaaa
     2461 acctaaatag ttcatttaat atatctgtta catttatttt ttctatagaa atatcatagt
     2521 aataatgtat tagataaagt aaatcctgtc taagtccctt aatatcagcc tctccattta
     2581 atgcatccat ttctatcaat agatatatta tcttgtcaac attcttttct acacctgcta
     2641 atgcaatttc atttaattgg tttaaagttt tattatcaat aattccaatc attccaaaat
     2701 ctatatatgc tatcttattt ttagcaacta cgaatatatt acctggatgt gggtctgcat
     2761 gaaaaaatcc atgagataat acctgtgtaa aaaatgacct aacgcctatt tcaacaatag
     2821 tttttgtgtt ataacctagt ctccttattt tttctacatc acttaacttt gtaccattaa
     2881 ctttctccat tactaaaatt ttcttagtat tatattctga atatacttta ggtatatata
     2941 cttcatcact atttttaaat atcctagaga atttagttgc atttatagct tcaaatgtat
     3001 aatcaagttc cctcattaat tgagtttgaa attcttcaat catctgtact aaatctaaat
     3061 taaaatcctt ttttaaatct tttaaagtat ttgcaatagt tctaagaatc tctaaatcag
     3121 attttattat tttctctata tttggtcttt gaatttttac gattacttct tctcccgttt
     3181 taagtacacc ttcataaact tgaccaattg aagctgctgc tattgcttct tctttaaatt
     3241 ctaagaatat ttcttctata cttaaaccca cttcttcttt aaatatatca atagctatat
     3301 ttgaatcaaa tttctctaca tcatctctga gttttgatat ttcatcaatt atatcctggt
     3361 ctaacaagtc ttttcttgta ctcaatatct gtcctatttt tatatatgta ggtcctagtt
     3421 cttctaaaac tcttttcatt ctctcaccag ttgtcatatt ttttatttct tctggtgggt
     3481 caaatagtgg tattttatag gcaattcctt ctatatttag tctttctaca ataaaactaa
     3541 aaccatactt tattaaaaca tatccaattt ctctatatcg ctttaaattt ctataactta
     3601 ttctcaaatc ttttcactcc taagataggt atacccaaaa acttacacct taatttatcg
     3661 tatatgacat tatttcacca attgatattc taatatccta tatcctcttt aatatttaat
     3721 tttatcacat atactaccaa ttttattaaa attatttaac tcagttaatt tttatttttg
     3781 acattttata tctggtttaa tcaaatactt tttgaaaact aaaatatcaa caaaatctaa
     3841 taaaataaat tagtggttgc ctcaaaacag atttaaatat gttttgaaac aaccactctt
     3901 taaactaata tttttgattt tgtgctgcct ctaaatacca ttcataagat tcatcaatat
     3961 attttttatc ttcttctgta agctctctaa caaccttacc aggagaaccc attatcaaaa
     4021 cacctggtgg aaacttctta ttagatgtaa ttagtgtacc tgctcctatc agagtatatt
     4081 ctcctatttc agcattatcc aatattattg aacccatacc aattaacaca ttatcactaa
     4141 ttttacaacc atgaactata gacctatgcc caactgttac attgttacca attatagttt
     4201 ctgtatctcc atgcactata gaacaatctt gtatattagt gttttctcca atagttattg
     4261 gtccttcatc acccctaact actgcattgt accatatact tgaatctttg cctattttaa
     4321 catttccaat tacatctgct gattttgcca caaaaacact ttcgtcaatt aaaggtttat
     4381 cttctaaata atctctaatc atatataaac aactccttta aaatataaat ttagttggtt
     4441 gctccttcta acattaactt agcttgtatc attatagagc actctgtacg ttttctttgc
     4501 atttcacatc ctaattcata tggttttgat atgtcattat taaagtttat cgcatttgca
     4561 tgacatccac ctgaacagta aaacttattc caacattgtt tacactcttc tttagtatat
     4621 acatgagctt ctctaaacat attttttaaa ttttctggaa gtactatttc ttcatcaaat
     4681 atatttgcca ttttaaattc ttcatttcca acaaattgat gacatggata gatatctcca
     4741 tttggagtta cagaaagata ctcatttcca gctccacatc cagttattct ctttattaca
     4801 catggacctt gatttaggtc tatcataaaa tggaaaaatt taaatccatc tccttgtaat
     4861 tgtctatctg catactctac agcaaatttt tcgtactctt caaatatctt aggtaaatct
     4921 tcttctctaa gtgcatatgg attactctca tctccaacga ctggctctac agaggttaat
     4981 ttaaatccta aatctgcaaa atgcataaca tcttttgaaa aatctaaatt atctcttgta
     5041 aaagtacctc ttatataata atacttatct tttgctcttt tttccacaag tttcttaaat
     5101 tgtggtaatg taatatcata acttccttta tcatttaaag ttggtctcat attatcatta
     5161 acttcttttc ttccatctaa acttaaaact acattatgca tattttcatt tatataatct
     5221 ataatctcat catttagcag tacaccattt gtagttattg taaatcttat atttttatta
     5281 taatcttttt ctacacttct tccatagtca accagttgtt ttacaacttc aaagttcatc
     5341 aaaggctctc caccaaagaa atctatctct aggttctttc tacttcctga attagcaatc
     5401 aaatagtcaa ttgctgcctt accaacttca aaactcataa gttctttttc tcctccaaaa
     5461 tcaccttgag ctgcaaaaca gtatttacat tttaagttac aatcatgagc tacatttaaa
     5521 cataaagctt ttactacagg ctctctatgt acaaagcttg gatgatattg atatgtatct
     5581 tctgtatata aaagaccctc tttttccaaa tttaatattt cttcatatgc ttcatttatt
     5641 ttttcttctt gatattttga ttttaatatc tctaatattt cttctttact tttttcttta
     5701 taaaaatcta acaaatcgta tgcaacatca tctaacacat gaacagctcc accattcaca
     5761 tctaaaacga tattatatcc attcatagaa aatttatgta tcatactcat accctacctc
     5821 ctaaattact atctatacta aagtttttta ttattttaat taaactttac ccttcattat
     5881 atcataagtt tacataaaat acatacaata caaaaatgac ttactctccg taagtcattt
     5941 cattaaatac actatatccc cctaaattgg cgcactaata tattaccaaa taggtattag
     6001 gctcctataa aataaataaa ataaattttg tatctgattt tatattgttt taatcttatt
     6061 aaaatataat aatttctttt ttcctgataa ttttgtataa ataagtgctt gtatttaatt
     6121 tgtcttcttt agattgctga tttctttggg tactttaggc tgatgaataa tccaaggaca
     6181 ggtactatta gctgacaaaa tagcagtgct aagtgccaat gagctagcaa acttcataaa
     6241 tcttacaata aactttttca taacttaact ctccctcata catattttac ttatacttta
     6301 gcttttaact tacctaatac taacatcaca aaaatccaaa acatggcaaa tgcgctatat
     6361 gtgaaataat ctacaaatat actcaaagaa agacttaaaa ttgttatcaa taacactact
     6421 gtagatataa atatagcagt tttcttataa accaactttt ccctatcact tatagggtta
     6481 ctcctatgtt ctattgggca cagaacacaa attccaaccc aactaacact tgcaataatc
     6541 ataataatat ttttaaataa gtctatattt atattttcta taattaagat tgtagataaa
     6601 attataaaaa taaaggttaa taagcatcta aaataattgt ctgcatgata ccctcctgaa
     6661 aattgtctta ttggacaata acacattaaa aaaaataata catatgtaaa tttaccaaat
     6721 aaaaaaccta taaacagcat ggtggttata ttaactatga aagctattaa tatttcaaat
     6781 ccataagaat agactttaga ttcattatta tctatcatat tattacaaat taaaacagat
     6841 gtcatttttt cagcatatct tttaaacat
//
LOCUS       DAGSHT010000179         6832 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGSHT010000179 DAGSHT010000000
VERSION     DAGSHT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.185"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(316..1662)
                     /locus_tag="MJB72_003017"
     CDS             complement(316..1662)
                     /locus_tag="MJB72_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8264092.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      1912..2014
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            2108..3484
                     /locus_tag="MJB72_003018"
     CDS             2108..3484
                     /locus_tag="MJB72_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBY8264093.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(3598..4743)
                     /locus_tag="MJB72_003019"
     CDS             complement(3598..4743)
                     /locus_tag="MJB72_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8264094.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVIVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            4996..5703
                     /locus_tag="MJB72_003020"
     CDS             4996..5703
                     /locus_tag="MJB72_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264095.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            5750..>6832
                     /locus_tag="MJB72_003021"
     CDS             5750..>6832
                     /locus_tag="MJB72_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264096.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVF"
BASE COUNT         2406 a          900 c         1010 g         2516 t
ORIGIN      
        1 ctgtaaataa aaactgtaaa ataaccttta ttggtgcaat gttaaatata aacgctagaa
       61 ttacaaaaaa caatgcaaca aaagtttttt caactgagtc tacagcccat atcaagatag
      121 ttaaaaaaga taagcatata gaaaatttta tgttgaaact gtaattaaga gagaagtaga
      181 gaattatggt agctataaca gtcatataaa attgttttgg cttaagaata atacccattt
      241 ttatattcac ctccataatt tattatatca taatggaggt gaatacaatg aatgtgaata
      301 cattttataa aaagattatt cattgattct atgatttaca atagctttag ttttccaatg
      361 tcctttctta tagtagataa ctgtaacaat aagacctaaa gtccaaccaa aaggaatacc
      421 catccaaatt ccgtctgttc caaaaaattt agaaagcata taagaagctg gaagtcttcc
      481 aagccataaa gatactattg agcttataag tgcaaacatg gaatcaccag cacctttcat
      541 tgcacttgaa agtacaaaag aagttccaat aaataagaag aatggtccta ttataaataa
      601 gtaagaagag ccaattttaa tgacatcttt atcagtattg aatagagcaa ttagattttc
      661 cctaaatagc aaaattaaag caataactgt aactgctaaa actaaggtca tttttataga
      721 ttctcgaatg ccttttttaa ctctttcatt ttctccagca cctatgtttt gagcaacaaa
      781 agtagaaaca gcagaaccaa gattcataat aggtaagctt ataaaatttt ctatcctaag
      841 accagcacca aaagctgcca tagcagatgt accataacta ttaactaaaa attgaagagc
      901 catatttcca attgaaaaca acatttgttg aactccactt ggaagtccta atctaagtcc
      961 atctttaaat attttgttat catatacaat tcccttgatt ttaaatttaa gcacttcatg
     1021 ttttttattt aaataaatta tacttattat aaatgatact gcctgagcca ttatagtggc
     1081 aagtgcaacg ccctcaacac ccattcttag cactacaata aaagttaagt ctaaaactat
     1141 atttagaatt gtggctatta tcagaaaata aagtggagtt actgaatctc caagtcctct
     1201 aagtattgca cttactgaat tatatccagc agaaaataat attccactaa atattatagt
     1261 aagatacaat tttgattgag caaaaacgga ttctggtgta ttcataagct taagcattgg
     1321 gccaccaaat actattccaa gaatacttat taatatagaa ccaataaata aaagtatata
     1381 tgtagtatca atagtagctt ttaccttttt taaatctcct gagccataaa attgtgacac
     1441 taaaatagaa aatccaaggc taacacccat aagcgctgcc accaataaaa acattatagg
     1501 gttagctcca gagactgccg ccattgcttc ttttccaata aatctaccaa ctattatact
     1561 gtcagctgta ttgtatagtt gttgaaataa actaccaata agcataggca tggcaaaaaa
     1621 gaatatggac tttccctcat gacctgtagt taaatctttc acattattcc tccaaataaa
     1681 aaaattatta atttattata gtataatctt aacataaact ttatatccaa actataaata
     1741 aattgtatca aaatatcata tattttttta aaatgtaaag aatataatta taatttgaat
     1801 ttaataaatc aatatttaat ataatcgaat attaatacaa aatcaataca atatatacgt
     1861 atatattgac aaaattaatt atataaatta aaataatatt cgtaaagaaa aattcatgtt
     1921 ttgtactgta taacatataa ttttgacaat atgggtcata agtttctacc ggaataccgt
     1981 aaatattctg actatgtata ctattattta gttaattaaa tatttaatta actgaattat
     2041 gaaggatacg gctcagctta tcaactactg tgtcgttttt ttgtaaaatt taataggagg
     2101 tgttgttatg caaacaacaa acagtagtac tatgcagaaa ctttttccaa ttttgactaa
     2161 taaaaatgtc gacatgaaga aggaaataat tgcaggagta actacttttt taactatggc
     2221 ttatataata gctgtaaatc caaatatttt atctgagaca ggcatgcctg caggtgcact
     2281 tgttacaggt acttgtttag cagcagcatt tggatgtatt ttaatgggtg tagttgctaa
     2341 ccttcctttt gctttagcat caggtatggg tcttaatgca ttttttgcat atacagtagt
     2401 tttacaaatg ggtgttcctt gggaagtagc tcttacagca gtatttgtag aaggtattat
     2461 ttttatagta ttatcagtaa gtggagttcg tgaggctgtt gtaaatgcga taccaaaaaa
     2521 tatgaaatta gctgttacag gtggtatagg aatatttatt gcacttatag gtctagttaa
     2581 cagtggaatt gttattggag accaagccac tcttatcaaa atgggtagat ttacaccagc
     2641 agtaataata acttgtgtag gattaataat tattgctgta ttagataaaa aaagagttaa
     2701 aggttctata ctttttggaa tagtagtgag ttcattactt gcttgggcat ttgcttttat
     2761 aaatccagag tatgcacaaa aattaggtat atatttacct ggtggattat ttaaattcga
     2821 aagtttagca ccaatagcag gtaaaataga tttaggatat gtactacatc caacaaatat
     2881 aggtggtttt ttagtagtag tttgtacatt tttatttgtt gatttttttg atacagtagg
     2941 tacattagtt ggtgtttgtt ctaaagcaaa catgttagat gaaaaaggga atgttcctaa
     3001 tgtaggtaga gccttactta cagatgcggt tgcaactaca gttggtgcag gacttggagt
     3061 atctacagtt acaacttatg ttgagagttc aacaggagtt atcgcaggtg gaagaactgg
     3121 atggacagca ataacagttg gaattttatt cttagctgca atgtttttct ctcctgtatt
     3181 tatagcaatt ccatcatgtg ctacagcacc agctttaata tatgttggat atttaatgct
     3241 tggtacagtt aaggatatag agtttgataa tataactgaa ggtgttccag cttttgttac
     3301 tattgcttgt atggcattaa catatagtat aggtgatggt ttaactttag gtatattaac
     3361 ttatgttttc gtaaatatat tttataatat atttggagct aaaaaggcag aagacaaaaa
     3421 acatgtttct gtagtaatga ttgttctggc aatattattt ataataaaac tttggctact
     3481 ataataggta ttatttaaaa ttttaattta atataaaata aagggctgta atttattatc
     3541 aattgtgatg ataaactaca gctcttttgt atttgttgta tttttgtatt tattgtatta
     3601 tttaataatg tctattagtt ctccattttg taaagtttta ggtgtaatta ctatattgaa
     3661 ctctttttgc aaatctttta taatatctaa ttcttttttt gttattattg agattgtgtt
     3721 accacttcga tttcctctag cagttcgacc acatctatgc aggtattcat ttttactttt
     3781 tggaaaatcc aagttaaaaa catgacttac atccacaata tctagtccac gagcagataa
     3841 atcagttgta attaaaatct ttgctttacc aagtttaaat ttatttattg cattctttct
     3901 atcttctttt tccatatttc caaaaattcc tattgctttg taattatgat aatttagttt
     3961 tgatactatg acttctatat ttttttcatt atttacaaat acaatagctt tttttggatt
     4021 tgtagccgct aatgcctttc taagaaaagt aaatttatcc cttatctcac ctaataaata
     4081 tgagtgattg atgttaggat ttatttgtga tttttccttt gtttttatta tttcaaactc
     4141 cttcataagt ttatcacaaa tttttatagt gctatcagta agacttgcag agcatcctat
     4201 aatttgtcta tctcttaacg ttgttcttat tatgtcttct atacaagtta tattttttcc
     4261 attcaataga ttgtccactt catctaagac aatagttttt atattatgtg attttagctt
     4321 cttttgtttt atcaaatcta aaactcttcc acaagagcca attactatgt gtggttttac
     4381 tgcttttata ttttttattt gtttttgtat attgacctcc ccaattaaag ctaaagaagt
     4441 aacggagagt tctgcatttt tagccaataa ttcaacctgg tttgtaatct gcattactaa
     4501 ttcatgcgta ggtgcaagta taagagcttg tgtttctctt tttgatgtat caattttttc
     4561 aaatataggt aaaaggtatg ctagtgtttt tcctgttcca gtctgtgaat taatcaataa
     4621 atccttattt tgaattatat tccctataac aagtgactga acttctgttg gagaagttat
     4681 gtcttgtttt ttgagtccat ctattagagt ggaacttatt tttaattgtt caaatgtatt
     4741 catattttac tccttatatt ttaataatta tagtcaacta ttttttatta tacattgttt
     4801 attcaaatcg aactatatat tcttttataa ttatgtgtaa tttgaaagta aatatcaatt
     4861 atatttatac ctttatattt ctgatacctt tgcattttat agatttctaa aataaacaaa
     4921 taaaagttgt aattgtttga tatttgttat atattatata taacaaatat caaaactata
     4981 atatggaggg aaattatgtt aaaagtaatc aatgtatcta aatcatataa aaataaaaaa
     5041 gtagtaagta gtgtctcatt tgatgtaaat gatggagagc tttttgggtt tataggacat
     5101 aatggagcag gtaaaactac tactattaaa gcaattgttg gaatacacga ttttgaagag
     5161 ggagaggtac taataaattc taaatctata aaaaaacagc ctgttgaatg taaaaaggaa
     5221 atggcatata tacctgataa tccagattta tacgagtcat taactggtat acaatacctt
     5281 aattttatag ctgacatatt tgaagttgcc aaagataaaa gagaagagtt aataaaatat
     5341 tattcaaata agtttgaact caataaagca ttaggagatt taatatcttc atattcacat
     5401 ggtatgaaac aaaaattagc tataatttcg gctttgatac acagcccaaa aatcttaatt
     5461 cttgatgaac cttttgtagg tcttgaccca aaagcttcct ttatattgaa ggaaattatg
     5521 agggagtttt gtgacaaagg tggttgtata tttttctcaa cacacgtttt agaagttgct
     5581 gaaaaaattt gtgataagat tgccataata aaagatggaa atataattgc ttatggaact
     5641 acagaagaag ttaagggaag taattcactt gaaagtattt tcatggaatt aatagagaaa
     5701 taaaatgttt tttatacaat atagtgtcta aagtgaggaa tgataaaata tgagcaatct
     5761 aagtatatta ataaaaaata atataataaa tgaatttaaa ttaaatgcgt taaaaaaagc
     5821 tgatggtaga gaaaaacaaa aaataatggg aatgatgctt tcagttgtta taatagcggg
     5881 tctattaatt ttttatgtaa cgagcttaag tctctcttta gttgatatct taaagcaaat
     5941 aaatcaactg gaaatgctac tgatatttgg ttttggatta tcaacattaa caactattgt
     6001 tacatcatta tataaaacgt catcatatct atttcaagca aaagatttag accttttgac
     6061 tagtttacct ataaaagaat ctactgtact agcaagtaag atattaatgt tggttggagc
     6121 aaactattta ttttcttcaa tatgtatgat tatacctgca gtggtttact ttttaaattc
     6181 tgatgtaagt ataacttatt tcccttattt gatattgcta tttttagctt taccattatt
     6241 accgatagtt gcttcatcaa taatttttct atttataggg cagatatcat caagaataaa
     6301 atacaaaaat ttagtattaa tcataggtag cattatattg atgttgcttt ataccctact
     6361 tatgtcaaga cttaattcta tgtcagttga aatgctaaag aatagtgtgt ctatttcaga
     6421 gacgattaga aaagtatatc ctcctatata ttattttgtt gatgctctta aaacaggaag
     6481 tatagtttca tttttagcat ttatagccat ctcaataata ccatttttta tgtttgtaac
     6541 tctgtttgca aaaaaattta aagatataaa tgctaagatg agtgaaggat ataagtcaaa
     6601 aaattatgaa tttaagggtc aaaaaacttc aagaccttca aaagctttat taaaaaaaga
     6661 atttaagaga tatacttctt cgtttatata ctttttaaat tcatctgcag gtttgttatt
     6721 tctaatagta ggtacaattt taatattatt atttggagca gataaagtgg gtgagttact
     6781 gaaattgaat atagatttta atcttataaa aatacaatta ttgggagtat tc
//
LOCUS       DAGSHT010000180         6800 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.186, whole genome shotgun sequence.
ACCESSION   DAGSHT010000180 DAGSHT010000000
VERSION     DAGSHT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.186"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..902)
                     /locus_tag="MJB72_003022"
     CDS             complement(<1..902)
                     /locus_tag="MJB72_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBY8264097.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIP"
     gene            1092..2963
                     /locus_tag="MJB72_003023"
     CDS             1092..2963
                     /locus_tag="MJB72_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBY8264098.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYVSLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(3055..3990)
                     /locus_tag="MJB72_003024"
     CDS             complement(3055..3990)
                     /locus_tag="MJB72_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBY8264099.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            4510..5022
                     /locus_tag="MJB72_003025"
     CDS             4510..5022
                     /locus_tag="MJB72_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBY8264100.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(5177..6238)
                     /locus_tag="MJB72_003026"
     CDS             complement(5177..6238)
                     /locus_tag="MJB72_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBY8264101.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(6362..>6800)
                     /locus_tag="MJB72_003027"
     CDS             complement(6362..>6800)
                     /locus_tag="MJB72_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBY8264102.1"
                     /translation="IKIPEELKFCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVA
                     LLISALLNKNFDLLKVACQDKLHQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTI
                     MSLIKENDDSFVDNMKNYLQKLKSDWEIKELCCDSNGAVLNII"
BASE COUNT         2572 a          863 c         1016 g         2349 t
ORIGIN      
        1 aaaggtatag atataactga aaatagtgtt gtaaaaaaca cacattgtga agctagtgtt
       61 atattggagt catattgatt agccattata gcagtatttg cagcagcagg cattgaagaa
      121 attactactg gtatagctaa aatcatctta tcatttatcc atccttttag aataaaatac
      181 acaatcacag gaagtaccaa taatcttata aaagtgacta tatataattt tttgttgaca
      241 aaacaatcga gagctgaaga attagcaagt aagcttccaa tgataatcat ggatattggt
      301 gtagttatac ttcccaacat ctcaagtggc tcgtttataa attggggtaa cctaagtcct
      361 gttacaaata aaaaaagtcc aataacaata gctattgtag cagggcttat aagtgatttt
      421 attgaaaaac ctctacttgt actgccttta gataaaagat aaattcctaa agtgaaagaa
      481 aataagttaa aaggcaaatt gaatattgca gtatagaata ttgcttcatg acctaaaact
      541 gcttcaatta caggataacc cataaaagca acatttgaaa aaacaactat atactgatat
      601 attcccaaat cttttttatc attacatttt aaaatgtatt tcaataaaaa tgcaattata
      661 attgaaacta tatacataat caaagatgta aataataaca atagtatttt ttgtatcatt
      721 ttactatcaa agttaatttg catggagcta attatcattg atggtaaaaa aactgtcatt
      781 gtcaaagttg aaagcttaga tgtgcagtgg tcatctaata agttgaattt tctgacaaaa
      841 tatccaacta aaataattat aaataaaact gcaacttgga taaaaatatt gctgaaattc
      901 ataaaatgtc ccccctatat atacattata taccaattac ataataaaat ctaatgatta
      961 taaaatttaa aatttaatgt tacaatatag tgacaaaagg taaaaattca atattatagc
     1021 attaattgta gattataatg tataataatg tttgagagaa tatattttat aaaatgggta
     1081 ggtagcgaat aatgaaaaat acaaaatttt ctataaaaaa aataaaaaat atatctaatg
     1141 acccgaaagt cgtacatact aagggtgccc tccttgaaaa gatgggtaag atggaaagcg
     1201 caatagagtt gtataagagt gctgcattgg ataattacgt aaaatctcaa tatactttag
     1261 gaaatatata tgaaagtaaa aaacaattta aagaagcaga aaagtggtat tcaatggcat
     1321 ataaaagtgg aagtgaagat gctgcttttg atttaggtaa tatgtactat aagttagatg
     1381 gctatgaata tgctatatat tggtatgaaa agatagcaaa tttaggttat ttacaagcac
     1441 aaaataattt aggtgtatgc cattttaaaa tgaaagattt tgtaaggtgt gaaaaatggt
     1501 taaaaacagc agcagacaag aatttaggta aagcttgttt taatttaggg gtattatata
     1561 cttttttgga gaaagatgat gaagcatatg aatactataa aaaaggttct gtgcttttag
     1621 atgatgatgc aaaatataat ttagcaatat taaatggaaa aaagaaagat aataaatcaa
     1681 cagtaagttt atataaacag ttgtacaagt caggtcatat gaaaggttgc tttaacttgg
     1741 gtatgattat ggaaataaat gataatttag aagatgcaga gaagtattat aaaaagagtg
     1801 ctgatagaga tgatgtgaag tctgagtata ggcttgcata tgtatatgat agaaaagaag
     1861 agctgggaga tgcaatttat tattatgaga aagcaataga aaaaaatcat acactttcta
     1921 agtatagact tgctaattta tataatagag aaaatgagat agagaaggca aaaacttatt
     1981 ataaaatggc tgctgaagat gatataacag aggcaaaaaa taatctggca ggtatatatt
     2041 ttgaagaaaa agattatgaa aatgctatta aatattatga ggatgctata gctgttggat
     2101 gcaaaagttc ccttgaaaat ctaggagatt tatattatca gaatcaagat atagaaaaag
     2161 cgatttcgta ttattcgagg attccaaata atgtatcttg tcaaatcaaa ttaggaaata
     2221 tctatgaaga tttaaataat attgaagaag caataagttg gtataagaaa gcatctgaaa
     2281 atggagatac gaggtctagt tatagattgg ggtgtatata tgagagctta ggaaatacaa
     2341 aaaatgctag aaagtatttt gaaatggcaa gtagcaaaaa ccatatgaat gctcgtatac
     2401 atctaggtag aatttatttt agagaaggta aattggaaga gtctaaaatg atgtttgata
     2461 cacctgccaa tgaaaataat gtgtatgctc aacatatggt aggattaatt tatgatatgt
     2521 tctataaaga ttatgtaaat tctaaatttt ggtatgaaaa ggctagagca caaggttgtg
     2581 ttgaatcaat atataattta ggtcaaattt atttaaaatt aaatgatgac gcagaagctg
     2641 agaaatatta taaagaaggt attaaatatg gaagtaagaa gtgtgagtat atgcttgcag
     2701 gtctttatta taaaaagagc ctagatatgt acgtttcttt agcaaatgaa gagtacgata
     2761 attgtgaaga gatagtagaa ggctttccta atttaaatat agactttgat gaagtattaa
     2821 ttcctccatt taagttgcaa gaagaggtaa tagatgaaga agagtatgta ccaatttata
     2881 tactaaatat aaaagaaagt ttagattcaa tgctagaagg gcttgaaact gaaatgatta
     2941 tagatgaaga acatgatagt taaaaagtat tataagatta tatagattta aatatataaa
     3001 agccattagg ataaatgtaa atcaattaat tctaatggct tttttattaa tattttataa
     3061 tagcttattt cttgacatga aaaatgtaat cactatacat attatagagg taataagaag
     3121 tattatccaa aatccgtttg ggttattaga aaatggcatg ttgatgacat tcataccaaa
     3181 gaatcctgat attattgtag gtattgagaa aattagtgta actactgtta aaatttgcat
     3241 aacattattc tgattattac ttataattgc actgaaagca tccataattc tactcaatat
     3301 atctccatat attttagcca tatcaagagc ctgtctattt tcaactataa catcttctaa
     3361 taggtcttca tcatctggat acttctttat accagcagtt ctaaccattt tgtttaaaac
     3421 aagttcatta gattttagtg aggtagagaa gtataccaaa gatttttcta attccaataa
     3481 ttgaaccaat tctttgtttt tcattgattt gtaaatttct cgctctatta ttattgtcat
     3541 tttatttatt tttcttaagt aatgcaagta ataagctgca tttttgtgaa gaatttgaag
     3601 caaaaagcga gtctttttaa atgtaaaaaa atctttaata tgaccaacta taaagtcatt
     3661 taaaatctta gtttgtgcat cacaaactgt tacaatagct tcttcagtta gtataatacc
     3721 taagggtatt gtagtataat gagaagaatt agagtcactt tcgtctactg gtatatctat
     3781 aagtataaga gtatgattgt cttcaacatc tattcttgag cgttcttctt catctagtgc
     3841 agattctatg ctttctacat ctatatttaa taaattagaa atttcgttga tttcactatg
     3901 attaggttca acaagattta tccaacagcc atcttcaaaa aaactaagtt tctctaattt
     3961 tgaatctata gttttgtaat atcttatcat ggcaacacat cctttattca gttttatata
     4021 aatctcatca tttaatagta tctattaatt aactaattta tataactgaa tatatgtatt
     4081 agttttgatt ttagatatta ttaagtgatg aaaatgtaaa aaattctctc cgcaacggac
     4141 tagtatccat tgcccatcac tctccttatt tattatttta tatatagcta tttatgaaat
     4201 gcgtatttta agcactcttt aatataatac cttttccagg tatataaatc aagttttgat
     4261 ttgattaaat aatattttta ccataattta cacataaagt atataagtta tatttaattt
     4321 gagtaaagaa tatatatatt ctgagtgaaa ttattaaata tattaaaaaa taagagagta
     4381 tatatatatt tacttatttg caataatata tttaaaaaag tattttattt tcaaaactag
     4441 acttcattta taatttatca cgaaaataaa aggaatataa atataagaga atgagaggtt
     4501 attagagata tgagtgattt taaagaaatt ttggcagaag agattaaaaa taatccattt
     4561 ggattgatag gtaaagattg gacacttata acagcagaaa atgaaggtaa agtgaataca
     4621 atgactgcta gctggggagg gcttggagtt atgtggggaa aagatgttgc ttttgtagta
     4681 ataagaccac agcgttatac taaggaattt atagataata cagataaatt ttctcttaca
     4741 ttttttgatg aagattttcg taaagagctt tcatattgtg gaaaggtatc tggtagagaa
     4801 gaggataaga tatcacagat tggatttaat atagaacact taaatgatac accttatttt
     4861 aaagaatcta aaatggctat tatatgcaaa aaaatgtata gtcagaaact tgaacctcaa
     4921 tgttttattg ctgaaggtat agatggaaga tggtatccac aaaaagatta tcatacttta
     4981 tatatagctg aaataaccaa tgtacttgta aaagaagatt agttataagg gttaatacta
     5041 tctctaatta aattaaaatt tcaataaaag gaaattgaca caaaaaagct aaggacataa
     5101 atatgtttat aaagattaat aaataacgta tttaaactcc ttagcttgat aaaatcttaa
     5161 tttagttttc tttaaattat aattcagaaa tcaattcatt gataacatct tcaacagtac
     5221 ttaatttatc aattttatag gcatcatttc cacaaaataa aagtgaattt tcaacatctc
     5281 ctttaactgc attgattaga gcttgagaaa tacagtaagg agtttctttt ggattacaag
     5341 gaatcaaaca attatagcac ttctttattt ctggacgatt tattttaact tcttctataa
     5401 atttgtttct tatggcgcgt ccaggaagac ctacaggact tttaactata tgaacatctt
     5461 cttcactagc atttatgtat gccattttaa agttctcatg ggcatcacat tcgtaagtag
     5521 caacaaatct tgtaccaact tgtacaccac ttgcacccat atctatatat tttttaacat
     5581 cactactaga agttatacca ccagcagcaa ctactggtat gtttctacca aatttttctc
     5641 cataagtatt tgcaaccttt aaaatttcaa caaattcctt atcatagtct atagaatcta
     5701 tgttatcaag ttcctcgtta gagtatccaa ggtgaccacc agcttttgga ccttcaacaa
     5761 ctattaagtc agcagtagtt ttctcttttc tatcccacat ttttaaaatt acattagcag
     5821 cttttgcagt tgaaactatg ggtgctattt ttacattact tccctttact aatgatggta
     5881 gcttattagg taatccagct ccagatatta taaggtctac accagcttta acagcttcct
     5941 ttacgtatgt ttcatattct ttcatagcta ccataaaatt aataccaata ataccaccat
     6001 ttgagatttc tttggcctta gaaatatgtt tttttatagc tcttaaattt gcatttatag
     6061 tattagtttc aaaatcttcc tcatcatatc caatttgaac tccagaaatt acaccaacac
     6121 caccaagctt agcaactgca ccagctagtg atgacctaga tataccaacg cccattccac
     6181 cttgaattat aggaactttt gcaactaaat ctctaattat taactctttt atgataatcc
     6241 ctcctcaatg taaacatgtt taaagaacta gattataata cttaatatta ttacctagtc
     6301 ctaattatta tactacaaat tataataaaa gagtagttat attttgtata gaagacgata
     6361 attatattat atttaaaaca gctccattag aatcgcaaca taactctttt atttcccaat
     6421 cagattttag tttttgtagg taatttttca tattatccac aaaactatca tcattttctt
     6481 ttattaaaga cattatagta ggtccagctc cacttaaaaa tactccaata ctatttaatt
     6541 gttcagattt ttcaacaata tcattgtagt tctcaattag agttcctcta taatcttgat
     6601 gcaatttgtc ttgacaagct actttaagta ggtcaaagtt tttgtttagt aaggcagata
     6661 ttagtaaagc aactctacta acattgaata ccccatcaga atatggtatt tcctttggta
     6721 aaacacctct agctttttct gttgataact taaaattagg tatcattgca caaaatttaa
     6781 gttcttcagg aatttttata
//
LOCUS       DAGSHT010000181         6705 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGSHT010000181 DAGSHT010000000
VERSION     DAGSHT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.187"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            227..418
                     /locus_tag="MJB72_003028"
     CDS             227..418
                     /locus_tag="MJB72_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264103.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(507..1319)
                     /locus_tag="MJB72_003029"
     CDS             complement(507..1319)
                     /locus_tag="MJB72_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBY8264104.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1741..2892
                     /locus_tag="MJB72_003030"
     CDS             1741..2892
                     /locus_tag="MJB72_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264105.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            3010..3648
                     /locus_tag="MJB72_003031"
     CDS             3010..3648
                     /locus_tag="MJB72_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBY8264106.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3649..4671
                     /locus_tag="MJB72_003032"
     CDS             3649..4671
                     /locus_tag="MJB72_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBY8264107.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            4661..5281
                     /locus_tag="MJB72_003033"
     CDS             4661..5281
                     /locus_tag="MJB72_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264108.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5405..6091)
                     /locus_tag="MJB72_003034"
     CDS             complement(5405..6091)
                     /locus_tag="MJB72_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBY8264109.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6105..6650)
                     /locus_tag="MJB72_003035"
     CDS             complement(6105..6650)
                     /locus_tag="MJB72_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8264110.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
BASE COUNT         2669 a          737 c         1014 g         2285 t
ORIGIN      
        1 atataatcta tagttttctt taagttttct ttacataaaa ctaagagttg gtctatgttt
       61 cattttatca aggcatattt attaaaaaat caatctctat ttgttaaaat aatttatgaa
      121 tgatgttaga aactgtaata tattggatat aatataataa acagataaac tctgtattaa
      181 gaggatatat agtttatctt aattataggg gaagtgagga tatattatgg aaagattaat
      241 ggaactcata gaaagaaaat ttttaaagga tgtagaactt ttagaattag aagacataga
      301 aaatgtaata gaatgcaaaa tgacacagcc atataatgct cattataatg aatatttagt
      361 aaaagtaaaa aatggagatg aagtagaaga atattttgtg tatttaaaac atagataaac
      421 ataaaagagg tttatattta tttgtatgat tataaaataa atacaagcct ctttttttga
      481 taaatataaa attataaaat ttttatttat ttaatttttt caacaacatt agtcaatatt
      541 gaatctaaat catcactaat ttctgtaact ccattatgag taatatctat aatttcttta
      601 gtcataggaa gttctcctaa aagctctaag tctaaatcat ctaaaaactt ttcagtagat
      661 tctccttcaa ataacttgat ttttttagaa caatcaggac attggatata actcatattt
      721 tcaataactc caaggacatt tatgttcatt tttttagcca tattaacagc ttttgataca
      781 atcatagata ccaaatcttg aggtactgaa accataacta ttccacttat aggaatagat
      841 tgcataactg ttaaggcaac atctccagta ccaggaggca tatctattaa taaatagtct
      901 aattcttccc aaagaacatc agtataaaat tgctttacaa cccctccaag taaaggtcct
      961 ctccaaacaa caggttcatt ttcatcatct atcataaggt ttatagacat tacttttatg
     1021 ttatttgagt tttctacagg gtaaatataa gagccatcag aataagcttt tacatttttt
     1081 acacccataa gtctaggaat acttggtcca gttatatctg agtctaatat accaacctta
     1141 tatcccattt tgtttagctt attagcaagt aaagcagtta cagttgattt tccaactcca
     1201 cccttcccac tcataacacc aattattttt ccaaatttat tgttagggtt attttcaata
     1261 gaacaatttg attgactatt acaatttccc tttgatggac atgaattaca atctgccata
     1321 tctattacct ccaaacttta atatttatta gattatttgt aacctacata tactatttta
     1381 gtacaattga accataaagt cattatttaa aagcaactta gaagattttc tattgattta
     1441 ttcatagact atagcaaaaa gaatacaata aaaaaattat aacattcttg aaaaagtgat
     1501 tatttcaatt tagttataga acttagtcat ataaatatat agaaaatatt ttaatatgtt
     1561 atacataacg ggttatttta taagtttaac tataaattag gtttatattt tataaaaatt
     1621 tgctaaaaaa aacaattaaa aattgtaaag aaaaaagata taattagaac tgtagtaata
     1681 aatgttttat tatagataat agagttttaa gtatcttaaa taatatttgg aggaatatga
     1741 atggaattaa gaccaagaag tgcaaaatta gaagaatttg ataaagtaat agaattaata
     1801 aactatgtat ttagaatatc aagaaatcat aaaccaacta tgatggaaga atttcctctt
     1861 ttactaagta aaaataatat agaaaatatg attataatat cagaagatga taaagtgatt
     1921 tctgatgtaa actatctaat tcaggatgta tctattcaag gaaacagatt gaaggttgca
     1981 gctataggtg gagtatgtac tcatccagat tatgaaaaga gaggctattc atctaagata
     2041 ttggataaag tagaagaaaa aatgttttat gatggtgtag atattgtaat tatatcagga
     2101 acaagaagtc tatacagtag aagaaattgt agtttggtaa agagttttta taaatataca
     2161 ataaaacctg aagatgtaaa aatagcatat gaaatagttg aatttgatga aactaatttt
     2221 gaaaaagata atgatttaga taaaatgatt gaactatata atcaaaatag tactagattt
     2281 ataagaacga gagatgagtt tcaaaaacta ttgcatgcag caactattgc ctggggacca
     2341 attggttaca agaaggtatt tataaaggaa aataataata taataggata tttgataata
     2401 agaacaataa aaaaagaaga ttctactgta ggagaagttg ctgaaatagg gttaaactct
     2461 gtaaatgttg aaaatatatt gaaatatgtt gcaaataaat ttggattaga atatttaaat
     2521 tataaagtac atgtgaaaaa tcttaaagac cagttgaaat gtaatgggac aaaatctttg
     2581 gattatcaac aaggaactat gaaaataata aattttacta agttgtgtga tagtttaaga
     2641 agttatttta gtcaatatgt agattttgaa ttactaaaat acatggaatt taaacaagtt
     2701 gaaaataaat atattattaa atacaaagaa gaagaacttg taatagaaaa tttggataag
     2761 cttaataaac tgttttttga aaaaaatgag gaacagtaca atgaatttaa acatctaaaa
     2821 aatatatatg aatttgctac aaaggcattt cctgtagatt ttccatggac tgcaaattta
     2881 aattatcaat agttttagat gatattaaat aaaattgatt gatacctaga gttagtcctt
     2941 gttaaataag agaaattatt ggatattgaa aaagcaattt tgtaagtaat tagaaaggat
     3001 gagtattata tggacatact tgatgatata gcagaatgta tattaaatat gggtgtagat
     3061 aatatagaga atttagtaaa aattgctata gataaaaata ttgatgtgga agatatttat
     3121 gaatatggtt taaacaaagg tatgatagga gctttagata agtttgagaa taaggaatac
     3181 tatctttcag aagttatagt atgcacagat gcattaaata aaggtattaa tcttttgaag
     3241 ggaactggaa aagtaaagaa aaaatcaaag ggagtaattc tgatgtctgt agtagaagga
     3301 gatactcatg agataggaaa aaatattgta aaagtaatgg tagaagccac tggatataag
     3361 gttattgact taggtgtaaa tagaaaaagt gaagatataa tagaagaagc tataaaaaac
     3421 aatgtagata taataggctt atcttcaatg atgacaacta caatggaaaa tatgaaatcg
     3481 gttatagacg aacttaatat gattgagata gataaaagac caaaagtaat aattggtgga
     3541 ggacctgtaa gtatggagtt tgcagaagaa ataggagcag atggatacag ttcaaatgct
     3601 ccaaaagcag ttaaattaat aaataaactt ataggaggtg aagtttaaat gttttcagat
     3661 atgactttac ttcagtatat aaatagtgaa gatagaggtt tttttctgcc tgatatgggt
     3721 acaaatggat tgttcttaat aaataaaaag gcatatgaag tatatgatag tgtagatttg
     3781 caattagaat tagcaaagac tatggacaag tattttgaat cagattttat atactcattc
     3841 tgtgatggga ttacattttg tgaaactctg ggtttagaga cattaagacc agattatgat
     3901 tttccaagtg tattgacaca tactataact gacagagata aattaagaaa attagatgtt
     3961 ccagaccctt atacgagtgg acgtatgcca ctcaatatag agagcttatc agttatatca
     4021 aaaagtatta aaaaaccact atatgaatcg atacaaggtc catttacact tgcaggtcaa
     4081 ttagctggag ctactcaact tttaagatgt ataataactg ataaaaaatt tgtagaagaa
     4141 ttattggaat ttacaactga attggtaaga agatatgctg tagctgcaaa taaagcagga
     4201 gcaaaatata tttcaatagc agagccaaca tcagtaaccc taagtaaaga tagatttgat
     4261 gaatttatag taaaaaattt aaataaaatc tacaatgaac ttgattgttg gaaaggtatg
     4321 catatatgtg gagacaccag agaattatta gataatatgc taagttgtaa tatagatgca
     4381 gtaagtttag accaaatttt agactatgaa gaaattgctc caatgattcc tagagatata
     4441 gttttgattg gtaatttaga cccaataaaa cttcttggac gttctaagcc agataagatt
     4501 agaagagaga cgctaaaatt gttaaagaag atgagaagat atgataactt tttatgtgat
     4561 tttggctgta actgcttaaa taccactcca gtagaaaatt tacaagcagc tataaaggca
     4621 ggaagaatta gttataaaga attagatagg attgatattg atgaattata atagtaataa
     4681 aaataaatat cttttaaagg atataaattt agatgatatt tttatagata agaatattgt
     4741 gctaaagttt ttggggtatg gaaatagaaa agctccagat acaattagta aaatcgttga
     4801 aagagaaata agaaatataa atgatatttt agatatcaag atatatataa gtgaagttga
     4861 tattaataaa acacctaaag aatgtaaaaa agcatttgct gtactatata caatagggga
     4921 taaaattgat attaagatga atgactatat ggaaaattgc aatatgatgg cagggctagc
     4981 attggataaa ataggtatag tatgtttaga ttatattaat gagaaaataa aaatgtattt
     5041 aaaaggaaag tatgatagtc tcaaaatatc gcatgaaata tatccaggag ataaagattt
     5101 tgaagtagag agacaaaagg atatttataa ctatgtaaaa aataaatata atagtataga
     5161 aatagaaatt aatgattatc atcaactatc ccccataaaa agtgttgcaa tgcttatttt
     5221 aatgggggat gaagaaaatt tagaatcaag atgtagtaag tgtcctagaa aatgctttta
     5281 gttaattttc aagactaaga tttgttttgt gtataaattg caataatttt tttggtcatt
     5341 ttatgtataa actaaaaaaa ttattgcaaa gtaatattct atttaatttg aacagactta
     5401 caaactatag tagtttcttt aattcaataa gtgtatttat ttcataagtt ggtatatgat
     5461 tagtataatt tatactgtta ttggaattta accaacaagt atctattcct gaatttattc
     5521 cacctaagac atcagaggtt aagctatccc caattattaa ggcagaagat ttatcatgac
     5581 aattaaactt tttgagagcg tatttaaata tttcaggatt tggttttgat attttcaact
     5641 cttcagatac aaccattcca tcaatatatc cttttatttt tgaattttca agtcttttat
     5701 tttgaactgc agaaattcca tttgttatta taacaatagt atactttttt ttaagatatt
     5761 gtaatatatc gattgcacct ggaattaaaa atgtacattc acctaaaaaa tttaagtaca
     5821 ttttgctaag agtttcagaa tcaacatcca aagatatttt atttgcaaaa agttcaaatc
     5881 taagtaattt aagttcattc aaagttataa tatttttttc aaggtcgagc caaagcttat
     5941 cacttatatt tctatatgac tctatataat tctcaaaatt aaattctaaa tcaaactcag
     6001 atagaagttt tttaaaagca tgtgattcac ttttcttaaa atcaaataaa gtatcatctg
     6061 catcaaaaaa tataaattta tatgtcttca tggtttttac tcctttacaa gatgactgca
     6121 aaaattactt tactttcact atttgagtca ttttgccatt tatgttttgt tcctaaagga
     6181 acttttacac tatctccttt ggacaaagta aattcatcat catccataaa aagtttaact
     6241 tctccctcca aaacatatgc aatttcatct ccattatgag taatcctatc aacacaagaa
     6301 gaagtgtttg gagctaaatc cataattgca aattctaaat taccactaga gtctggaatt
     6361 aaaatctcat atataacttc tttgcttcca ggtaaaataa ctttttttct attatcatgt
     6421 cttactatca agtttttttt atctacttca gatacaaaaa atgtaaataa aggaacattt
     6481 agagaagagg ctatagattt taatgaattt acagatggat tagcaagacc tcgttctatt
     6541 tggctcaata atgatggtgt aacatcagct agttttgcta aatctcgaat acttaagttt
     6601 tgtttttttc gaacttcagc tattttttcc cctaaattta aatcattcat ataaatatcc
     6661 tctcttttta atttaaaaaa tattaactta aaataatcaa gtaaa
//
LOCUS       DAGSHT010000182         6692 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGSHT010000182 DAGSHT010000000
VERSION     DAGSHT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.188"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..135
                     /locus_tag="MJB72_003036"
     CDS             <1..135
                     /locus_tag="MJB72_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBY8264111.1"
                     /translation="NAVEDKKRVWNKVDSYYVEDGNTISIGDILLDNEGDLLYFNNIN
                     "
     gene            146..2467
                     /gene="cas3"
                     /locus_tag="MJB72_003037"
     CDS             146..2467
                     /gene="cas3"
                     /locus_tag="MJB72_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBY8264112.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFDNLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            2482..3033
                     /gene="cas4"
                     /locus_tag="MJB72_003038"
     CDS             2482..3033
                     /gene="cas4"
                     /locus_tag="MJB72_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBY8264113.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            3036..4016
                     /gene="cas1b"
                     /locus_tag="MJB72_003039"
     CDS             3036..4016
                     /gene="cas1b"
                     /locus_tag="MJB72_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBY8264114.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            4019..4285
                     /gene="cas2"
                     /locus_tag="MJB72_003040"
     CDS             4019..4285
                     /gene="cas2"
                     /locus_tag="MJB72_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBY8264115.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   4469..5674
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4469..4497
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            5863..6291
                     /locus_tag="MJB72_003041"
     CDS             5863..6291
                     /locus_tag="MJB72_003041"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBY8264116.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT         2792 a          630 c         1094 g         2176 t
ORIGIN      
        1 aatgctgtgg aagataaaaa aagggtttgg aataaggtag atagttatta tgttgaggat
       61 ggaaatacta tatcaattgg agatatatta ttagataatg aaggagattt attatatttt
      121 aataatataa attaggaggg agagtatgtt atatgctaaa tctaatccag tggaaacact
      181 tagagagcat actgatgaac ttttaaaaca aatgaatgta ttaagagaaa gttatggaaa
      241 aaatataaat agcttagatt ttctagaaga ggagatattt tggcaattac ttgactttgt
      301 tatagaattt catgatattg gaaaggcatt ttcaccgttt caagagttaa taaaaagcag
      361 aatggatact aaaataaatg aacctaaaat agtaactcat ttagaaaata atgtaggcca
      421 taattatctt tctcctgcat ttattgatta tagttatata gacaggaaaa aaaataaaga
      481 acttagagca gtattaaatc aagttattgt atatcatcat gaaagagata tctttataga
      541 taaagatttt aaaaatttaa tacaaaaaat attagatgaa gatttaataa ataaagttta
      601 tgaactacag catgaattta aagttagata tccaataaaa acagaaagat tgagtaaagt
      661 atatctacaa agtgtagaaa aaagaataga taaaaatcac aagtactata atttatatat
      721 tatgttaaaa ggaatactac atagactaga ccatagtgct tctgctcatg aagttgtgga
      781 atgcaataat gttataaata taggagaaca gacagaaaat tatttaatga aagagtttga
      841 taatttgaga gaggctcaat catttgctaa atctaataga aataagaata taatactgat
      901 tgcatctaca ggaatgggaa aaacagaaac tgctttaatt tggatagata aagacaaggc
      961 attttttaca cttccattaa gagtaagtat aaatgcatta tttgatagag caaaaaatat
     1021 agttggagtt ggagaatcaa atgatacatt cttaggtttg ttacattcaa cagcaataga
     1081 ttatctagaa gaaagtaatc aagaaaattc aagtgagatt gtagatttag caaagttatt
     1141 atcttgcaaa ctgacatttt ctacaataga ccaaatattt aaatttcctt ttttgtatag
     1201 agggtatgaa aaagtgtact caacattagc atattcaaaa gtagtaatag atgagataca
     1261 agcatattca ccagaaatag cagctgtatt ggtaaaaggt atagaaatga tacataagat
     1321 aggtggcaga tttatgataa tgacagctac tatgccaacc atatatatag atgaattaaa
     1381 aaaaagaggt gtaatgaata gtaacttagc tgaattgaca tgtaatacag aaaaaataag
     1441 acattgtgtt tctattgtag aaaattcaat agatgaaaac ttaggtaaaa taatacaaag
     1501 tggaatgaat aaaaaagttc ttgtaatagt aaatacagtt aaaagtgctg tagaaaaata
     1561 tgagttaata gaagaaatag ttaaaccaaa aggaatagat gtcaacttaa atcttttaca
     1621 ttctatgtat atacaagaag atagagcaaa attagaaaaa tatataaaag aatttgccaa
     1681 tagtgataga aatggaattt ggattacaac acaattagtg gaagcatccc ttgatattga
     1741 ttttgatgag cttcatacag agaattcaac acttgacagt ctatttcaac gatttggtag
     1801 atgttataga agtcgtgagt atgaagaaaa tagcccaaat atatttatat atacagaaaa
     1861 agctacagga ataggtagta tttatgataa ggatattgta gaaaaaggac ttgagctact
     1921 gaaaactttt ataaatggaa aagaatgtgt gaagatgaaa gaaaaatata aggtagaaat
     1981 ggttaagata ctatactcta aagaaagtct agaaggtaca gcttttgaaa agagatttac
     2041 cagtgcttta aatattttag atactataac gccatatagt ttaggttcta aagatgcaca
     2101 aaatatactt agaaatattg aaggatatac agtaattcca gaggatatat ataataaaat
     2161 tgaagaaact ttaatcaaag attatgaaga gttgggacaa gagcttataa atgcatatag
     2221 taatgatgat agacaattga taaataaaat aaagtctaag agaaaaaaag caagaagaga
     2281 gataattaaa aaaactgtaa atttaccaat atataaagct aaacaaaatg taattgacat
     2341 acaggtgaaa ggtcttgaag atttaaaaat acttttatat aaatatgata tatacgaaaa
     2401 taagcataca aaacagatat ttggtaaagg tgtgcttata agtaatgaaa tagaccaatt
     2461 tatttgattt ttatattaac aatgtggcta aaatttacta aagaaggtga ttttgtgcca
     2521 atagatttag aagtattaaa agttcaaggt gtaaaaataa attattatta tgtatgtaaa
     2581 agaaaactat ggctattttc taaaggaata accatggaac aaaatagtag tagagtattg
     2641 tctgggaaga tagtacatga agattcatat aaaagaatga aaaataaaga aatattgatt
     2701 gatgatatct tgaagctaga tataataaag gatgagtatg taaaagaagt aaaaataagt
     2761 tcaaagatga aagaagcaga tgaaatgcag ttatactatt acttatacta tttaaagaat
     2821 agtatgggta tagagaaaaa aggaactata aattatgtta aggaaaagaa acaagaagaa
     2881 ttgatattga cagaagaaaa agaaagaatt attgaaaaca cacttataaa tataaatgaa
     2941 ataaatatgc agttatctcc tcccaaaatt gagaaaaaac attattgtac aaaatgtgct
     3001 tattatgaat tttgttttgt gaaggaggag taacaatgta taaagactat tatattataa
     3061 gtaatgggga tataaaaagg aaagaaaata cgatttacta ttacaattta gaaggtgaga
     3121 aaaaaatatt accagtagaa caagttgatg atattcatat atatggagaa gtagatttaa
     3181 acacaaaact tataaattac ataagtaaat atggggtgat gcttcatttc tacaattact
     3241 atggatatta ttcaggtaca tattatccaa ggaagaaaaa tgtatcagga ttcagtttag
     3301 tatgtcaatc agcctgctat ttagataaaa atgaaagatt atacttagca aaatcctttg
     3361 tggaaagtgc atcataccat attttaagaa atatgagata ttacaaagtt gatgaaagtt
     3421 taattgatac tataaaggat gaactaaatg atttgaatga tgtcaaagaa atatcagaac
     3481 ttatgggtaa agaaggcaga ataagaaaaa catactataa atcatttaat caaatattaa
     3541 aagatgattt tgaattttat aaaagagaaa aaagacctcc caaagaccca gtaaatgctc
     3601 ttatgtcttt tggaaatagc ataatgtata cgaatgtttt aagtgagata tataagacac
     3661 aacttgaccc tacaataagc tttttacatg aaccatcaac aaagagattt tctttaagtt
     3721 tagatattgc agaaattttt aagccaatta tagttgacac aataattttt agcctgatta
     3781 ataataaaag aattaataag aatgattttg aatatcaaga caaaatttgt tatttaagtg
     3841 aaaaaggaaa gaagaaattt ttgcaagaat ttgaaaataa gatgcagaca actataaaac
     3901 atagaaatct aaatagaaaa gtttcatata gaatgtttat aaggttagag tgttataaat
     3961 taattaaaca ttttataaat gatgaaagat ataaagtttt aaaggtgtgg tggtaagtat
     4021 gtttgttatt gttacttatg atattgttga agcaaggtcg ttaaatagaa ttagaaggat
     4081 acttagaaaa tatttgactt ggacgcaaaa ttctgttttt gaaggcaata ttactgaagg
     4141 aaagttacat aaatgtattt ctgaaataga aaatattatt gataatagcg aggattcaat
     4201 ctatgtttat gagataaaaa atcctaattc aattaaaaag aaatgttatg ggattgataa
     4261 gtattctgat gaaatgttta tataggtttg cagtgagcga tatttatgct aaaataggtg
     4321 ttaacagttg gaatataagg gattgaaggt gtatgataac tgttatcaat tgcactactg
     4381 ctcgctcact gcaaattttg atgtttttat tgaattataa ttgcttgatt gaagtatttt
     4441 caatgtattc aattatacct attttggggt tttatattaa ctaagtggta tgtaaatcta
     4501 taaaaataaa tcatacaatt tcacctcaca cttggtttta tattaactaa gtggtatgta
     4561 aattttacaa cgagacacat cagttatgtt agtgtttaaa gttttatatt aactaagtgg
     4621 tatgtaaatt cataaatgac gactgtacct tttgtaaaat agcaagtttt atattaacta
     4681 agtggtatgt aaatctgaac ctactaacat ggtagggttg aagtctacaa cgttttatat
     4741 taactaagtg gtatgtaaat aaatttccaa acccgtcgaa tccatggggt ttttcaccgt
     4801 tttatattaa ctaagtggta tgtaaatgca atatctcctc ttgtaatcgc tgaataaaga
     4861 tttcgtttta tattaactaa gtggtatgta aatttttttt cgcttgtttc aactctgatt
     4921 gagccttgtt ttatattaac taagtggtat gtaaatttgc aaatgtctta ggagtgtcgg
     4981 ctgatgaatt gcgttttata ttaactaagt ggtatgtaaa ttttcgatat ataggaataa
     5041 tttcttctgt attcactgtt ttatattaac taagtggtat gtaaattcca aaactacagg
     5101 ttcgtagaaa tcttcccaat gtgttttata ttaactaagt ggtatgtaaa ttatttcagg
     5161 tgtagtattg tatataccag tttcatttgt tttatattaa ctaagtggta tgtaaataat
     5221 agactttctc aaaataaaaa ggcaattaaa atcgttttat attaactaag tggtatgtaa
     5281 atttatcatt ttttccgctt ctgcttgtaa tttagactcg ttttatatta actaagtggt
     5341 atgtaaatac tgttatttta gcaacactta aaaatttaca ggacgtttta tattaactaa
     5401 gtggtatgta aatagaactt catctatcgc aatcgagctt tctacaatag ttttatatta
     5461 actaagtggt atgtaaatat aagtaaaacg ggtccgagcg ctgctgctaa tcctggtttt
     5521 atattaacta agtggtatgt aaatctaaag caactaaagc atatgattta tctaacaacg
     5581 ttttatatta actaagtggt atgtaaatag gataaagaaa agactcacac aaggcacagt
     5641 gtcaggtttt atatcaacta agggtagata agtagaatat tagcaatatc aacgagtatt
     5701 tagaaactta ttttatatta ataaagcagt atataaaatt gtagaatcaa caatagcata
     5761 tactaaaaca tccaccttat attaactaaa tcatatctaa ataaaataca cttacctata
     5821 aacattataa aatcaataca aaaatgaggt gaaataaaat ttatgataaa gaaattaaac
     5881 aataaagaca taaataaaat catggaaata tgggaaaaaa gtacaatcaa agcacatgac
     5941 tttataagta aagaatactg gcaaaataac tataatactg ttaaaaacga atatatacct
     6001 atatcagata catttgtata tgatgatgga gatgaaataa aaggatttat aagcataata
     6061 gataaaagct ttataggagc tttatttata aagcccaaat accaaaatct aggtatcgga
     6121 ggtaaacttt tagattatgc aactaaaaaa tataaaagtc taagcttagc agtatataaa
     6181 gataataaaa aagcagttgt gttttataat aaaaaaggtt ttaatatagt aaaagaacaa
     6241 gtaaatgaag attcaggatt taaagagtac ataatggaat atagtaaata atatgattac
     6301 attctattaa aaggataatt aatttatttg ccatttgatt ttttaagagt aatttttaaa
     6361 atagactata tgaagataaa gatatttatt atagatgtaa gttaatcaga attgatatag
     6421 ctacaaatga ggaaaataaa attgtatagt gtatttcaga tttaacaaat ttactattta
     6481 aagataataa gtttgatata atattaaaga tactttcacc tataaactat agggtatttg
     6541 aaagaatttt aaataattat ggaatgctaa taaaggtttg ttaaaattta ataattatta
     6601 gattatagaa aattgccatg aaattagttc atcaagaact agtttcatag caattttaat
     6661 tcttataagt gtatttattt ttacagattt aa
//
LOCUS       DAGSHT010000183         6679 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGSHT010000183 DAGSHT010000000
VERSION     DAGSHT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.189"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1232
                     /locus_tag="MJB72_003042"
     CDS             <1..1232
                     /locus_tag="MJB72_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY8264117.1"
                     /translation="GAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANITQTVDF
                     MANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIAISTMVL
                     AGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNTIAVADN
                     FSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSLFKSLCG
                     PIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKMQGVCIL
                     LLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAGATIASS
                     TFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFKLIPYEA
                     LIGIVMTIISTSIQLVL"
     gene            1319..3313
                     /locus_tag="MJB72_003043"
     CDS             1319..3313
                     /locus_tag="MJB72_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY8264118.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            3313..4635
                     /locus_tag="MJB72_003044"
     CDS             3313..4635
                     /locus_tag="MJB72_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBY8264119.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            4914..6200
                     /locus_tag="MJB72_003045"
     CDS             4914..6200
                     /locus_tag="MJB72_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBY8264120.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            6286..>6679
                     /locus_tag="MJB72_003046"
     CDS             6286..>6679
                     /locus_tag="MJB72_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBY8264121.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLD"
BASE COUNT         2668 a          663 c         1225 g         2123 t
ORIGIN      
        1 ttggagctat atttggttta ttaatagcaa ttattttaat tataaaaaaa tttcaagctg
       61 tatatagttt aatgttagga gcatttattg gaggattagt tggaggagca aatataacac
      121 aaactgtaga ttttatggca aatggagcta tgaatatatc accatcaatt cttagagctt
      181 tggcatcagg tgtattagct ggaagtttaa taaaaacagg ggcagtagat aaaatatcag
      241 aacagattgt aaaaatattt ggtgaaaaga gggctttatt ttcgattgcc atttcaacta
      301 tggtacttgc tggagtaggt gtaaatttgg atgtatctat aataactgta gcacctatag
      361 gattatatat aggtagaaaa ttaaattatt ctaaattgtc aatactatta gctatgttgg
      421 gagggggtaa agcaggtaat ataatatcac caaatccaaa tactatagca gtagcagata
      481 atttttctgt caatctctct agtgttatga tggcaaatat aattccagca attattggag
      541 ttgttattac agttatttta gcaagtatat taataaataa aggaaataaa gtacaatcat
      601 atgaaatttt agagcaaaga gaagatttac caagtttatt taaatcatta tgtggtccaa
      661 ttatagctat atttctattg tttttaggaa atgtatctcc tatagttata gacccaatga
      721 tagcccttcc aataggagga atcgtaactc taattgtcac aggaaacttg aacaattcaa
      781 gagaatattt aacttttgga ttatctaaga tgcaaggggt atgcatatta ttacttggaa
      841 cagggactat cgcaggaatt atacagatgt ctgaattaca acagagtaca ataggtgctt
      901 tacaattttt aaatatgcca caatttttat tggcaccagt ttcgggtata cttatgtcat
      961 tagcaacagc atcatcaaca gctggtgcta ctatagcatc atctactttt catgatgcta
     1021 taataaatgg tgggctatca ccgatttcag gtgcatcaat agtaaatgct gggtctagtg
     1081 tattcgagca attaccacat ggctcactat ttcatactag tgcaggtagt ataaacatgg
     1141 atataggtga aagatttaag ttaattccat atgaagcatt gataggaata gtaatgacta
     1201 taatttcaac atctattcaa ttagttttat aataaatata aatatttaat gaaaaaagta
     1261 ggattttgtt attaaatatt gaacttatta tagtaaaaaa gaagattgga gttttaaagt
     1321 gaataaaaaa aagattgtta ttatagggat tatttattca tttttagtag tattttcact
     1381 tacaaatatg tatgtaaata tggagtataa tctaaatgta tttgaatata ttaagaagtc
     1441 acttccattt acagaagaag aaaaaaagtg gttagaaaaa cacaagaatt tgatttacag
     1501 ctctgaccaa agttcacctc ctttgagata taaaggtaag gaagatggac agtataaggg
     1561 aattattgtt gatttaataa attctctgtc aattcaaatt ggtagagatt tttattttaa
     1621 accaaataat tggtggaaag aatctttcgt aaattcaata gatgatagta taaagttttt
     1681 tgacttgatt ccatcaaaag aaagggcaaa taaatttata tttactgacc caatatacac
     1741 tttgagtgca aatatattga aagataaaaa gtcccaagat ataaatagtt acatggattt
     1801 aaaggggaaa acagtagcaa ttccagaagg tgattattct ataaattttt taaagcaaaa
     1861 aattcaagat ataaacattt tattgactcc agatataaag actggagtca accatttaat
     1921 gtctggaaaa gtagacgcag ttgttggtga tgaaccagta ttgcgatact atataaataa
     1981 ttatggactt tcaaataaat acagtgtttt aagtaatcct atatatacta aaaaagcagt
     2041 tcttgctgta cctaaacaat atgaagagct ggttagtatt ttaaataaag gcatttttaa
     2101 acttcagaaa aatggagtgt ataaagattt aaagaaaaaa tggtattcta cgtataatga
     2161 agttgatgat atattgtatg agcgaggaat tgtgcctagt atttatcttt ttataggtat
     2221 aattttaata tcaatttatg ttttttatag ctatacatat ttgttaaaga tagaaatcaa
     2281 aaggaaaaca gaacaggtaa tagaaaataa aaaaacttta gaggcaactt ttaatagtat
     2341 tacagatata ataatgcttg tagatgaaaa taacaatata gtagaatcga acaaagtact
     2401 atatgacttt atgggtgaaa tgagttataa gatagcagat ttgattagca tgataaaagg
     2461 tgttattgaa aataccttct ctgaaaatac taataaaact agtgaaatag aaatacacaa
     2521 taaaatttta aagataaaca cttttcctgt agagtataaa aaaaataata ctgagtatat
     2581 agtagttctt attaaagata taactaatga taaaatagta gaggcgaaat tacttagaga
     2641 aaacaaaatg atatctatag ggcaattagc ttctggggta gcacatgaga taagaaatcc
     2701 tttgggtata ataagaaata attgctatct cttaaaagat aacgttacaa tggaagaagt
     2761 aaatgattgt gttaaatcaa tagaaagtaa tgtagatagg gcaagtaata taataaccaa
     2821 cttgttaaat ttcgcaagaa tatcagatga taatttagaa catataaata taaaaaattt
     2881 tattgaaaat atagtgaaac ttcaatataa aatgttacag ttaaagaatg ttgaaataaa
     2941 aattgactgt gaacataatt taatatgcta tataaatgga gaatcgttaa aacatgtatt
     3001 tataaattta atttctaatt caatagatgc aatacatcaa gatggaaaaa ttattatata
     3061 ctgttatgaa aaaaatcatt gtttatttat agattttaaa gataatggtg aaggaataaa
     3121 agaagatgtc ctaaaggaca tatttaatcc cttctataca acaaaaccaa ttggtgaggg
     3181 aactggttta ggtttatata ttacttataa tgagattaaa aagaataatg gagatataag
     3241 tgttgaaagt aaattggggg ttggaacttg ttttcatata aaaattccat taaataaaga
     3301 ggtgactata taatgaaaat actattagtt gatgatgagc tagaatatgg agtggtaatg
     3361 aaaaaaattc tacaaaagaa gggctatctt gtagatatag cactaagtgg tgaagaagct
     3421 ataaatataa ttaaaaaaga taaaaattat gatttggttt tatctgatgt tatgatgaaa
     3481 aatatggatg gagtacagct attagataga attaaatcta ttaatagaga tatagaggtt
     3541 atattagtta caggatatgg aagtattgaa aatgcagttg atgctatgaa aagaggagct
     3601 ttatcttatt ttataaaaag taatcccatt gaaaatttat tagaagaagt tgaaaaggta
     3661 aaggcaagta aaatatctgt ttctgtacaa aaaaataatt ttgaatttac tttggaatct
     3721 aaaaatagag attttaataa tatcataaaa atagcaaaaa aagctgcttg taaagatgtt
     3781 aatatattaa tcttggggga atctggtgta ggaaaagata tattagctag atatatacat
     3841 agcataagtc caagaaagaa tgagatattt gtacctgtaa attgttgttc tttttcagaa
     3901 aatctattgg agtctgagct gtttggtcat gaaaaaggtt cttttacagg agcagtagat
     3961 agtagaaaag gtagatttga actttcgaat aaaggaactc tatttttgga tgaaatagga
     4021 gatatacctc taaatgttca ggtaaaactt ttgaggacac ttgaggacaa atctatagag
     4081 aggataggaa gtaataaatc tattaaagtt gactttaggt taatttgtgc tatgaataaa
     4141 gaaccaaaag tggaaatatc aaatggaaat ataagagaag atttttttta tagaataagt
     4201 acaataacaa taactatacc accactaaga aagagaagag aagacttaac tactcttata
     4261 gaatttttct taaataagta ccaaattgaa catgataaaa agatacactc gattgataag
     4321 gaagtaaagg attttttatt aaattacaat tatccaggaa atataagaga attaaaaaat
     4381 ataataaata gacttgtagt tttaagtgaa gaaggtaatt tatcaaaaga caatttaaat
     4441 ttaatatcaa ataatgtata catagatgat aaaataagta ttagaccttt aagagaaata
     4501 agaaaagagt ttgaatgtga gtatattgaa aaagttttat ctttgtgtgg aaataatatt
     4561 tcaaatacag ctaaaaaatt ggagattagt agaagacagt taaccaataa aatatcagag
     4621 tataatatta agtgagaaaa aaacttctca ttaagtgaga aaaaaatttc tcaaactgta
     4681 ttaaaagata aacagaaaaa atctgtttat ctttttttat tataatttta aaaattaata
     4741 tttacaataa ttattgctaa attcaatatt ggtgtaaatt aaaaatgaaa atttctatga
     4801 cattaataca tagaatatat attttaaata attggcatag aaaatgcttt atatattaat
     4861 aaacgtaata aatactttta attgcgatta aatatattaa gggggatttt catatgaagc
     4921 tagagctagg gaatatattt ataaaaaatg ttgaatttgg agaaaagaca gaagtaaaag
     4981 atggagtact atatattagt agcgaagaaa tagaaaagat agtattagaa gatgaaagga
     5041 ttgtaagtgt aaatgttgaa cttgcaagac ctggcgaatc aataagaata gctccagtta
     5101 aagatgtaat agagcctaga gttaaaattg gagatgaaag caaaatattt cctggaatta
     5161 taaataaagt gaagactgta ggaagtggaa gaacccatgt acttttagga gcttgtgttg
     5221 taacttgtgg aaatatagtt ggatttcaag aaggcgtaat tgatatgagt ggtcctactg
     5281 ctaaatatac acctttttca aaaacaaata atatatgtat tgttataaaa gcaaaggatg
     5341 gtatagatac tcatgattat gaagaagcag ctagaattgc aggattaaaa ataggtgcat
     5401 atgtaggtga ggcaggaaga gaagttgagc cagatgaagt cattgtatat gagacaaagc
     5461 cattattaaa acaagtaaaa gaatatccag acctgccaac agtagcatat gttcacatgt
     5521 tacaatcaca aggactttta catgacactt attactatgg ggtggatgct aaacagatag
     5581 tgcctacatt tatgtatcca actgaaataa tggatggagc tataatatct ggaaactgtg
     5641 tagcaccatg tgataaggtt acaacattcc atcatttaaa taatccagtt atacatgact
     5701 tatataaaag acatggaaaa gatttaaact ttataggagt tattttgact aatgagaacg
     5761 tattcttagt cgataaagag agaagctctg atatggtagc taaattaata gaatttttag
     5821 gagttgatgg agttctagtg acagaagaag gttatgggaa tccagatact gacctaatga
     5881 tgaattgtag aaaatgtagt gaagttggag caaatgtagt tttgataacg gatgaatttc
     5941 ctggaaaaga tggcaagagt caatctatag cagacgctac taaggaagca gatgcagttg
     6001 tttcttgtgg tcaaggaaat ctagtggttc atttcccagc aatggaaaaa attataggaa
     6061 ctttagatta tgttgaaatg atgataggtg ggtataaagg atgcttaaat gaagacggaa
     6121 gcatggatgc agagctacaa attataatag catcaactat agcaaatggc tataatcatt
     6181 tgacagcaag atattattaa ataagtattt gaatataaag ttctgttgat gtaatcattg
     6241 gtctaactga tgtaataact ataaagtgat ttggaggatt aagagatgag taaaatcaga
     6301 gtggttcatt atataaacaa tttctttgct ggtataggtg gagaggaaaa agcagatatt
     6361 ccgccagaaa aaagggctgg tgtagtagga cctggaatag catttcagag tcaatttaat
     6421 gatgatgcag agataatctc aactgtaatt tgcggagata cgtattttgg tgaaaatatt
     6481 gagagtgcaa ctcaagaact tttaaaaatg ataaaagaag aaagccctga tttatttatt
     6541 gcaggcccag catttaatgc aggtagatat ggagtagctt gtgggactat ttgtaaggca
     6601 gttgaagaag agttaaatat tcctgtaatt acaggcatgt ataaagaaaa tccaggtgta
     6661 gatatgttta aattagatt
//
LOCUS       DAGSHT010000184         6670 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGSHT010000184 DAGSHT010000000
VERSION     DAGSHT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.190"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..627
                     /locus_tag="MJB72_003047"
     CDS             <1..627
                     /locus_tag="MJB72_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264122.1"
                     /translation="CEISVYKSVNTADYFDEWKVLGKTVIEIRKTEIMDMHLDGYRLS
                     KDLIISYLYDPILDKARKQKISESQKIIDKSNNIISKHKNLNKENNVKENNSKEKIRE
                     KEYKEFDDKVNYNIKKNLNNLNENNILIKEYVDKRTDKQKRSDKRNGREPKKELVYFD
                     VKANKEARIIYAVIMKNSKRAYRSIPAIIGKYLRDDGYKVGVAGKKRK"
     gene            689..3670
                     /locus_tag="MJB72_003048"
     CDS             689..3670
                     /locus_tag="MJB72_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBY8264123.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVEDLFRFL
                     EITQKELLDEFKYNRGSNYKKAFIDLINSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKQDRNPKETLFGFNQRVFVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     MGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKKSKRAIFPRFHQLDAV
                     RKIVADLEVNKAGKNYLIQHSAGSGKTNTIAWTAHRLSSLHDEDNNAIFDSVIVVTDR
                     KVLDKQLQDAIYQLEHKNGLVVKIDDDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLEKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKIIDAENEKNEKSSIDKMN
                     DLIEKRTNLDHISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPVSVYYKVGKKIEDNPEFDKGEAKRAINKFISLNEHNIRQKVETIVDDFVDNR
                     SMWLEGRAKGMIVTASRLHAVKYKLAIDQYIKEKGYNMKSLVAFSDTVKDEDIEYTEV
                     GMNKEVAPDINDSNLPSIFEKSDFRLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKHTFILDFQNSVEDIQEAYKPYFEMKNIDKVTEPNVVNDLWYQLHEY
                     GIYTDEEVNDFAVLFYKDKRTNTQDAKMNSLIDKAVERYCYLDEEDEKSADEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLRKLPKKGKERIDIDDILSLDYYKLQKL
                     GEDGKKGSDISLYNGEGELVGISETATSRVSEADEEYLEDIIQKINDVFGVGLTEEDR
                     IIIDQYEEMFKNDKSIMDIAKANSYEDLVSTFGKSYFKRGIVKTKNMNDRLVKEILTN
                     NNLQNYMIHYLAEKIYAEANR"
     gene            3688..4125
                     /locus_tag="MJB72_003049"
     CDS             3688..4125
                     /locus_tag="MJB72_003049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264124.1"
                     /translation="MTDKIIKPKNLFIYELKCDLIEDDERFVIKGKLDKEEINNLIFY
                     LQVEFRNILDNSILIPYDISNILTRLYGFEEVQNIEDISIDQTIELTDNIDENIYKID
                     QILENRSNLYVDGLYDMLEGIKLESVQDNPTVFEEEYVKLQNK"
     gene            4293..4628
                     /locus_tag="MJB72_003050"
     CDS             4293..4628
                     /locus_tag="MJB72_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264125.1"
                     /translation="MINLFKKEDLNLLLEYPKEVVENVDNVINILDESYGDNRKITDN
                     GGYVCIIEDIKEIEYLKSHILNGLIEEFSDVIYESEVDIYNSTLYLLSSDYSITIISK
                     NEETEHLLK"
     gene            4692..5153
                     /gene="radC"
                     /locus_tag="MJB72_003051"
     CDS             4692..5153
                     /gene="radC"
                     /locus_tag="MJB72_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBY8264126.1"
                     /translation="MDKKKIPAKRVDIVSLKLVKESSVLYETRKISNPYDAYRLVKNF
                     LVDSDREKFVVVYLDTKNQPVSIEIVSIGTVNSAMVHPREVFKVAVLSNASKIICFHN
                     HPSGNTNFSKEDEIITKRLQKCGEILGIELVDHIVVGDDDKYFSFKENFKI"
     gene            5294..5587
                     /locus_tag="MJB72_003052"
     CDS             5294..5587
                     /locus_tag="MJB72_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264127.1"
                     /translation="MFKKENRYMTREIAENLLIEITILLWDLIDNLDIEKDYLQIFEI
                     NPIGLGVVEIVHRKEVPEYEASIYIQNDLIKYKLKIYAIDSIKYSTMMFSNEY"
     gene            5614..6210
                     /locus_tag="MJB72_003053"
     CDS             5614..6210
                     /locus_tag="MJB72_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264128.1"
                     /translation="MINNKSEIINKLFFNELKELIDKCNNIEDKTVYMIEKIFISIED
                     EEIKNYLIRNNCKLQELVNRYKKLELLNIDEAIFFAWYNLNINTISIDKASQYYYELT
                     TSNYIEVESHLVYTNEIDLREYAKDELDTMLDMEYHIDRLLDKDMIIDMWLNNTSKED
                     VIEEILMSDDIEDILDISPEYAFTTTSGIEYRYSEKEE"
     gene            6406..6621
                     /locus_tag="MJB72_003054"
     CDS             6406..6621
                     /locus_tag="MJB72_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBY8264129.1"
                     /translation="MVDILGEEIVIKLYKYYRGQQITFPMKLYSNEYVERYIEKNYRT
                     KTLKDMCRELGYTEGWIKQLINKYKLK"
BASE COUNT         2934 a          602 c         1123 g         2011 t
ORIGIN      
        1 tgtgagatat cagtttataa atctgtaaat acagcagatt attttgatga atggaaagtt
       61 ttaggtaaaa cagtaataga aataaggaaa actgaaatta tggatatgca tttagatgga
      121 tatagacttt ctaaggattt aataatatct tatttatatg accctatttt agataaagct
      181 agaaagcaaa aaataagtga atctcaaaag attatagata agtcaaataa tataatatct
      241 aaacataaaa atttaaataa agaaaataat gtaaaagaaa ataattcaaa ggaaaaaata
      301 cgtgaaaaag aatataaaga atttgatgat aaagttaatt ataatataaa aaaaaactta
      361 aataatttaa atgaaaataa tattttgatc aaagaatatg tagataaaag gacagataag
      421 cagaaaagga gcgataagcg taatggacgt gaacctaaaa aagaattagt atattttgat
      481 gttaaagcaa ataaagaagc aagaattata tatgcagtaa ttatgaaaaa tagcaaaaga
      541 gcttatcgaa gtatacctgc aataatagga aaatatttaa gagatgatgg ctataaagtt
      601 ggtgtggctg gtaaaaaacg taagtagtta atgattaata taagctttat aatatataga
      661 ttaacaatat agaaatgggg gaatagcaat gttaccaaaa gagaaacatt tagaagagtc
      721 tattgaaaat catttagttt caattggata cgaacaaggt cattcacaag aatttgatct
      781 tgaaaattgc ttattcgtag aagatttatt tagattttta gaaattactc aaaaagaatt
      841 actagatgag ttcaaataca acaggggaag taattacaaa aaagccttta tagatttaat
      901 taactcaaac atcaaaagaa gggggcttat aagtgtactt agaaatggtg tagaagatat
      961 aatgatgaac ggaaaattcc aattattcta caacaaacca aatctaacta ctaacaaaac
     1021 agcttacgaa aattataagc aaaatatatt caaaataaca agacaattaa aatatagttc
     1081 aaagcacaac aatactttag atatagtatt atctttgaac ggatttcctg ttatagtaat
     1141 ggaacttaaa aaccaattca ctaatcaaaa tgtgtataat gctataaaac aattcaaaca
     1201 ggatagaaat ccaaaagaaa ctttatttgg atttaatcaa agagtatttg tatattttgc
     1261 agtagatact gacgaagtat atatgacaac agagttaaaa ggagataaaa cattcttctt
     1321 accttttaat aaaggtaaca atatgggtaa gggaaatcct gtagtagaag gaaaagaaaa
     1381 aacttcttat ctttgggaag aaatacttac taaagatagt ttacttgata ttataaagag
     1441 attttacttt atccaagaag atgataagaa aagtaaaaga gctatatttc ctagatttca
     1501 tcagctagat gcagtaagaa agatagtagc ggatttagaa gtaaataaag caggtaaaaa
     1561 ttatcttatc cagcatagtg caggtagtgg aaagactaat actatagcat ggactgctca
     1621 tagattatca agccttcatg atgaagataa caatgctata tttgattctg taatagtagt
     1681 tacagataga aaggtacttg ataagcaact tcaagatgct atataccaat tagaacacaa
     1741 aaacggatta gttgttaaga tagatgatga taaaaattca tcagatttag ctgatgctat
     1801 tgtaaacaga gcaaagatta ttattacaac tatacaaaag ttccaatatg cattacctaa
     1861 gatagaacaa cttgaaaaaa gacgttatgc agtaataata gatgaagctc attcttctac
     1921 atctggtgag aatatgaatg cattaaaaga aactttagca ggaaaaacat tagaagaagc
     1981 taagataatt gatgctgaaa atgaaaagaa tgaaaaatct agtatagaca agatgaatga
     2041 ccttatagaa aagagaacta atttagatca tataagtttc tttgcattta ctgctactcc
     2101 taagaataag actatgcaag tatttggtag aataggagaa gatggactac ctcatgagtt
     2161 tcatttatat tctatgaagc aagctataga agaaggattc atattagacg tattaaagaa
     2221 ctttatgcca gttagtgtat attataaggt aggtaaaaaa atagaggata atcccgaatt
     2281 tgataaagga gaagctaaaa gagctataaa caaatttatt agtttaaatg aacacaacat
     2341 acgtcagaaa gttgaaacta tagtagacga ttttgtggat aataggtcta tgtggttaga
     2401 aggcagagct aagggaatga tagttacagc aagtagatta catgctgtta agtataaatt
     2461 agcaatagat caatatataa aagaaaaagg gtacaatatg aaatccttag ttgctttttc
     2521 agatactgta aaagatgagg atattgaata tacagaagtt ggtatgaata aggaagtagc
     2581 accagatatt aatgattcaa atttaccaag tatttttgaa aaaagtgact ttagattact
     2641 tatagttgca gaaaagtatc agactggttt tgaccaacca aagttatgtg ctatgtatgt
     2701 tgataagaag ttagatggtg taaaaactgt tcagacttta tcaagactta atagaacata
     2761 tcctaataag catacattta tcttggattt ccaaaatagt gtagaggata ttcaagaagc
     2821 atataaacca tactttgaaa tgaaaaacat agataaagtt acagagccta atgtagtaaa
     2881 tgatctttgg tatcagttac atgaatatgg tatatatact gatgaggaag taaatgactt
     2941 tgcagtatta ttctataagg ataaaagaac aaatacacaa gatgctaaga tgaatagttt
     3001 aattgataag gcagttgaaa gatattgtta tttagatgaa gaagatgaaa agtcagcaga
     3061 tgagtttaag aaaaaatgtc aaaagtatat tactttttat aatttcttaa tccaaattta
     3121 ccctataaag aacttaaatc ttttaaggtt acaggtttac ttagtagcac ttttaaggaa
     3181 gctacctaaa aagggtaaag aaagaataga tatagatgat attttaagtt tagattatta
     3241 taagttacaa aagttaggtg aagatggtaa aaaaggtagt gatatatcat tatataatgg
     3301 tgaaggtgaa ttagttggta tatctgaaac tgctacttct cgtgtaagtg aagctgatga
     3361 agaatattta gaagatataa tccaaaaaat aaatgatgtc tttggagtag gacttacaga
     3421 ggaagatagg attataatag atcaatatga agagatgttt aaaaatgata agagtattat
     3481 ggatatagct aaagctaaca gttatgaaga tttggttagt acttttggaa agagttattt
     3541 taagagaggt atagttaaga ctaagaatat gaatgataga ttggttaaag aaatattaac
     3601 taataataat cttcaaaatt atatgattca ttatttggct gaaaaaatat atgcagaagc
     3661 aaatagataa ataaaaagga gtacaatatg acagataaaa taataaaacc taaaaattta
     3721 ttcatatatg aattaaagtg tgacttaatt gaagatgatg aaagatttgt aattaagggt
     3781 aaattagata aggaagaaat aaataattta atattttatt tacaagttga gtttagaaat
     3841 atattagata attccatatt aataccatac gatataagta atatattaac tagactatat
     3901 gggtttgagg aagtacaaaa tattgaagat atatcaatag atcaaacgat agaattaaca
     3961 gataatatag atgaaaatat atacaaaata gatcaaatat tagaaaatag atcaaaccta
     4021 tatgtggatg ggttatatga tatgctagaa ggtataaagt tagagagtgt acaagataat
     4081 ccgactgtat ttgaagaaga gtatgtaaaa cttcaaaata aataaggaat ctagtaaaat
     4141 gtaataatta taattatata ttataatttt gaaatataca ttaaatagat atcatactaa
     4201 agaggaacat acaaaaaata acacattgag atgttggtag agatactaac atctcttttt
     4261 ttagttcaaa aaattataag gaggaaatta aaatgattaa cttatttaag aaagaagatt
     4321 taaacttatt attagaatat ccaaaggaag tagttgaaaa tgtggataac gttataaata
     4381 ttcttgatga atcatacgga gataacagaa aaataacaga taatggtgga tatgtgtgta
     4441 taattgagga tattaaagaa atagaatatt taaaatcaca tatattaaat ggattaattg
     4501 aagagtttag tgatgtaata tatgaaagtg aggttgatat ctacaattca actctatatt
     4561 tactatctag tgactatagc attactataa tatctaaaaa tgaagaaact gaacacttac
     4621 ttaaataagc atatacattg ataaagtgta tatgcttatt ttaattttga gaaacaaagg
     4681 aggaaataat catggataag aaaaaaatac ctgcaaaaag agttgatata gtatcactta
     4741 aactagtaaa agaaagtagt gttctatatg aaactagaaa aataagcaat ccttatgatg
     4801 cttatagatt agtaaagaac tttttagtag atagtgatag agaaaagttt gtagtagtat
     4861 atttagatac aaaaaatcaa ccagttagta tagaaattgt aagcatagga actgtaaatt
     4921 cagcgatggt acatccaaga gaagtattta aagtagcagt attaagtaat gcatctaaaa
     4981 taatatgctt tcataatcat ccatcaggta atactaactt tagtaaagaa gatgaaatta
     5041 taactaaaag gctacagaag tgtggtgaaa tattaggtat tgaattagta gatcatattg
     5101 ttgttggaga cgatgataag tattttagct ttaaagagaa ttttaagata tgagagtctt
     5161 agcttaaaga aattaggcta agactttttc tatgttctaa actaattgaa ataaattagt
     5221 tttaagttaa gcccgaaaag cccttgaaaa aataatatac atataaataa ttaaactaaa
     5281 taggagttgt aaaatgttta aaaaagaaaa tagatatatg acgagagaaa tagctgaaaa
     5341 tctactaatt gaaataacta tattactatg ggatttgata gacaacttag atatagaaaa
     5401 agattatcta caaatatttg aaataaatcc tataggatta ggagtagttg agatagttca
     5461 tagaaaagaa gtacctgaat atgaagctag tatatacata cagaatgact taattaaata
     5521 caaattaaaa atatacgcta tagatagtat taaatacagt actatgatgt tttcaaatga
     5581 atattaacta ataattaaag gagaacgagt aatatgataa acaataaatc tgaaataatt
     5641 aataagctat ttttcaatga attaaaagaa ttaatagata aatgcaacaa catagaagat
     5701 aaaactgtat atatgataga aaagatattc ataagtatag aagatgaaga aataaagaat
     5761 tacctaataa gaaataattg taagttacaa gagttagtaa atagatacaa aaaacttgaa
     5821 cttttaaaca tagatgaagc tatattcttt gcttggtaca acttaaatat aaatacaata
     5881 tctatagata aagcaagtca atattactat gagttaacga caagtaacta tatagaagta
     5941 gaaagtcatt tagtatatac aaatgagata gatttaagag aatatgctaa agatgaatta
     6001 gatactatgt tagatatgga atatcacata gatagattat tagataaaga tatgataata
     6061 gatatgtggt taaataatac atcaaaagaa gatgtgatag aagaaatatt aatgagtgat
     6121 gatattgaag atatattgga tatatctcca gagtatgcat tcacaacaac tagtggaata
     6181 gagtatagat attcagaaaa agaagaataa aataaatata tgtgttgaaa aaatacgggc
     6241 tttaagggct tataccttaa agtatgtata aaaaatacaa tagattttac aatagaaaat
     6301 cagaatgaaa ctttttctaa tactaattat tattaatgag tatcagaaaa ggaggtggag
     6361 taatgatcaa gaataatagc gactatcaaa gggatatatg cagatatggt agatattcta
     6421 ggtgaagaaa tagtcattaa attatataag tattatcgtg gacaacaaat aacatttcct
     6481 atgaagttat actctaatga atatgtagaa agatatatag aaaagaatta tagaacaaaa
     6541 acattaaaag atatgtgtag agagttagga tatacggaag gttggataaa gcaattgata
     6601 aataaataca aattaaaata aataccctag ttattgttaa attattcaat aaaaaatatg
     6661 attttattac
//
LOCUS       DAGSHT010000185         6566 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGSHT010000185 DAGSHT010000000
VERSION     DAGSHT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.191"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1206)
                     /gene="hflX"
                     /locus_tag="MJB72_003055"
     CDS             complement(<1..1206)
                     /gene="hflX"
                     /locus_tag="MJB72_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBY8264130.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVELASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRYKFLIPYDKGHVVSYFNENTS
                     IINTEYREDG"
     gene            complement(1931..3763)
                     /locus_tag="MJB72_003056"
     CDS             complement(1931..3763)
                     /locus_tag="MJB72_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264131.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(3756..6002)
                     /locus_tag="MJB72_003057"
     CDS             complement(3756..6002)
                     /locus_tag="MJB72_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264132.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(6070..6531)
                     /locus_tag="MJB72_003058"
     CDS             complement(6070..6531)
                     /locus_tag="MJB72_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8264133.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
BASE COUNT         2078 a         1212 c          702 g         2574 t
ORIGIN      
        1 tccatcttct ctgtattctg tatttataat gctcgtattt tcgttaaaat aagaaactac
       61 atgaccttta tcatatggaa ttaagaactt atatcttaca taatctttaa atatgctatc
      121 acaaatacat tttatcattt tatctatatt tatatctttt ttaactgaaa caaatatacc
      181 ttctttatcg atactatcta aaattctatc caatacttct acatctatta aatcaatttt
      241 attatacaca tgtatcatag gtataccatc tgcacctatt tgttttaaag tatcttctgt
      301 cactttaata tgacttttat agcttggatt tgaaatatca ataacatgta acagtacgtc
      361 tgcctcacat gcttcttcta atgttgacct aaatgctttt actaaatcat gtggtagatt
      421 acttacaaaa ccaacagtat cagataataa aaattctttg ttatttgcaa gaactatatt
      481 tctgacagaa gtttcaagtg tagcaaataa catgtttttc tcaaacactt tcttttcttc
      541 agagttttta aatatatcta ctagtttatt cattattgaa gatttaccag catttgtata
      601 cccaactaga gctacatttg gaagatttga ttttcttctc atacttcttt gagtttctct
      661 ttggaatttt aaatcatcca actccctatt taaagatgtt atcttttctt ctattcttct
      721 acggtcaagt tctaattttt tctcaccaga acctctattt ttagtaccaa ctccaccact
      781 ttgacgtcct aaattttcat ttgaacctat aagtcttggt aacatatatt ttaaacttgc
      841 taattctact tgaagtttag cttctctagt ttttgccctt tgtgcaaata tatcaagtat
      901 caatgccgtt ctatctataa tttcacaatt aagcttttct tcaagatttt ttagttgtga
      961 tgtagaaagt tcattgtaaa atataactat ctctatattt tctttcttta ttaagtttaa
     1021 tatttcctca actttacctg tcccaatgta aaatgctcta ttaacctgct ttgcattttg
     1081 agttatactc cctacaacat caatatcaca tgctgaacat aagttttcaa gttcaatcat
     1141 tagttcatta aagtcctcag atttattatt tatgtttaat cctactgtta ttcctttttt
     1201 cgccatatcc atattctcct tatctgctat tttttattta atctatagca cgagattatg
     1261 gctctataat taaattcatc aagaattttg ctttttatct tatccttaaa tgacacttct
     1321 tatttactat tataataacc ataatcgtgc attattttct caatcttata taaaaaatca
     1381 ctttcttaca cctcctccca aatatattat gcagtttttt caataaaaaa actccttaca
     1441 aatctaagta atacaaaaca agctacttat atcataagaa ggattaaaat tctaaatata
     1501 tattaaaaat acattagttt aattaactta tatgtaatta tcattttata tttaacccta
     1561 ttcttaaatt taagtagtta tttgcatgat taataaaagt aataaaaaca tatacttcaa
     1621 tataatcatc aactattaaa attaaattct atttataata aaatttaaag aataaaatta
     1681 aatatacatt ttgataatca cctccaacat tttccttact acatattata ataaaataaa
     1741 cactttgtaa agacataaaa ttatgtggct atctcaaaat atagagaatc ctctttttca
     1801 ttgttaaaat gataaaaata cactttattt catacacaaa agagtgcctt atgaattaaa
     1861 aagttcattt taaggcaccc tcatttattt tatcttatta attacatgtc tatgctaaga
     1921 tagttttttg ttacattgct tcatcttctt caaattgaga attgtaaaga ttggcataga
     1981 atccgccttt ctttagaagt tcctcatgat ttccttgttc aactatatct ccttcattca
     2041 ttacaagaat catatcagca tcacgtatag ttgataatct gtgggctata acaaaacttg
     2101 ttctaccttc cataagatta tccatagctt tttgaatcaa tacttctgtt ctagtatcga
     2161 cagaacttgt agcttcatct aatattaaaa ttttaggatc agctaaaata gcacgagcta
     2221 tagtcaataa ttgtttttga ccttgagaaa cattacttgc ttcttcattt aatttcatct
     2281 tatatccatc tggtaatgtc ttaataaatc tatgaacatg tgcagattta gcagcctcta
     2341 taacttcttc atctgtagca ttcaacttac catatcttat attttccata attgaaccac
     2401 taaataacca tgtatcttga agaaccatac caaacatttc tctaagctca ctacgattaa
     2461 aatctttaat attatgacca tcaatcagta ttgaaccgct attaacatca taaaatctca
     2521 ttaacagttt aactattgta gttttacctg ctcctgtagg tccaacaata gcaacttttt
     2581 gaccaggttt aacatttact gagaaatcat ttattatagt cttgtttggg ttatatccaa
     2641 agttgacatt ttcaaaatca attttacctt ctaaaccatc tattgaaact gcattttcaa
     2701 caatttggac ttcttcttcc tcttctaaaa actcaaatac tctttcagaa gctgctgctg
     2761 ttgattgcaa taaattggca acctgtgcaa tctgagtcat tggctgtgta aagtttctta
     2821 catattgtat aaatgactgt atgtcaccaa cctcaattgt tttcttaatt gcaagccaac
     2881 ctcctaagat tgataccatt acatatccca agtttccaat aaacatcata agtggttgca
     2941 ttatacctga taaaaattga gatttccacg ctgaattata caggctatca tttagtttat
     3001 caaactcttc aattacttca tcctcacgat taaatgcttt tactattgtt tggccactgt
     3061 aagtttcctc aacttgacca tttacatttc ctaaatattc ttgttgatat ctaaagtatt
     3121 tttgtgattt ctttattatt cttgaaatta caaacattga tattggtaat attaaaactg
     3181 ccactaatgt cataattcca cttatagata acatcattat caaaacacct atcatagttg
     3241 ttactgatgt tataagctgt gtcatacttt gatttaaact ttgatttaaa gtatctatat
     3301 cattcgtaat tctcgataat atttcaccat gcgtcttagt atcaaaatac ttcattggca
     3361 atctatccaa ttttgctgaa atctcttttc ttaaattata ggatactttt tgagaaattc
     3421 cactcattat aaatccttgt ataaaagaga acagtgcact tatcaaataa aggaaaagta
     3481 aaaaggttaa gatttttcca attgcattaa aatctatacc tacgtttcct ccagaaactt
     3541 tactcactag cccttcaaat atctttgtag ttgcctttcc taaaattttt ggacctacta
     3601 tagaaaacga cgcactacca atagcaaata caattactag aattattgat attttaaatt
     3661 tagataaata tgcaattagt tttcgcatag tccctttaaa gtcatttgct ttttgtcctg
     3721 ctcccataga ttttcccata ggacctcttc ccattctact cattagctag ctcctccttt
     3781 gaaagttgtg ataaagctat ttgctcataa acttcgcaat tttttagaag ctctttatgt
     3841 gttcctattc caactatttc gccctcattt aaaacaatta tctgttctgc atttaagata
     3901 gtactaattc tttgtgcaac tattagaact gtactttcag cagtttttga ttttaaagcc
     3961 ttacgaagag ctgcatcagt ttttaagtca agtgctgaaa aactatcatc aaatatataa
     4021 acttctggat tttttgctat tgctcttgca atagaaagtc tttgtttttg acctccagat
     4081 acatttgttc caccttgaga aatttcagtt ttaaaagctt ccggtttaga atctataaat
     4141 tctgtagctt gagctatttc agctgctgtt ctcatttctt catctgttgc gtcttttcta
     4201 ccatatttta agttggattc tatagtacca gaaaacaata ctcccttttg aggtacatat
     4261 ccaatttttt cccttaaatc atgttgagaa acatttttta tattaactcc accaagtaat
     4321 atttctcctt cagtaacatc aaagaaacga ggaattaaat ttataagagt tgacttacca
     4381 ctaccagtac ttcctataaa ggcagttgtt tctcctggct ttgctgtaaa agatatattt
     4441 gttaatatag gttcttctgc gtctggatac atgaacgaaa catttttaaa ttcaacaaga
     4501 ccctttttat cttccttaaa ctcttgtgcc tctgattttt ctgggtcttt tatgcttata
     4561 tctgacacta atacttcatc tatacgtgta gctgaaacgg atgctcttgg aagcataata
     4621 gataccattg atatcattaa gaatgacatt attatttgca ttgtatattg tataaatgcc
     4681 atcatatctc caacttgcat tgctccagaa tcaacactgt atgcacctct ccaaactata
     4741 agaactgtga taaggttcat tataagcatc atagcaggca tcatacatgt cataactcta
     4801 tttacaaaaa gatttgtttt agttaaattt ttatttgctt tatcaaatct ttcttcttca
     4861 tgtttttcag tactaaaagc tctgataact ggaataccag taattatttc acggcttact
     4921 aagtttaact tatcaacaag cttttgaacc agtttaaact taggcataac cactgagaat
     4981 aacactacaa ttaatgataa gatagcaact actgctactg ctataatcca ggccattgaa
     5041 gtatttgtgt ttaaaacctt tatcactcca cctatagcca ttataggtgc atagaatact
     5101 attctaagca acataaccat caacatttgt atctgttgaa tatcatttgt actacgagta
     5161 attaatgaag ctgtagaaaa ttcattcatt tcagtatttg aaaatgacat aacttttcta
     5221 aatactccac ttcttaaatt tctacctaca cttgctgcta ctcttgctgc taaaaatcct
     5281 actgttattg tagctaccat actaatcatt gctataccaa gcattttagc accagtaaat
     5341 aatatataat tattttgagt tttttctgta tcaataccta ttttctcata ttctgacctt
     5401 acatatgaaa cagagctttg tgttatcata ctctctggca tactttcaaa gcctttgctc
     5461 atctctttag tcaatgtatc taactgttcc tttggcattg ctgataacaa tttaaatata
     5521 tcaacatctc cactttgagt agcttgtggt ggaagtttgc tcattaactg agctttcata
     5581 gcttttacct ctttactatc gccctcaagc cctgatacta ttaaaatagc tttaccaaaa
     5641 ataacattta aatcatctat agtgtctttg tctttagtat ttaactcata aagagattca
     5701 tcttttagac ctgggtactt atctttatac ttaacataat cctttttatt cagcaaagta
     5761 taattatcta gtactttatc cttactactt ttatccataa ataaagtaat tttatttaat
     5821 tcactctctc taatcacaga tggaacagag ttttctactc caccttgttg tatacctaca
     5881 tttactatat tggatgtgta ttctggtaaa gaaaggtcac ataccgcttg aattaccaat
     5941 agacagacta ttattactat tgataatgcc gatttcttta aatatttaat taatttaaac
     6001 atacatttct tcccttctgt aatattaatt agtctaataa cattaaattg aaaaacatta
     6061 ttttaaactt taaaaatatt tttcatcttc atgcttatga catcctgctt ttaaattatc
     6121 tcgtacttgc attagaagtc ttctaagtac tattttttct tcttcactaa atcctaaaaa
     6181 aaactctctt tccaattcct tgttaaattc ttcaacttta ttacatattt ctaaaccttt
     6241 gtctgtcaaa aaaatcctag atactctttg gtccttttca tcttgttttc tttccacaag
     6301 tccagctttt tccatcctct ttatcataac tgtagttgta gatgctttta ctttcctttt
     6361 atctgctaat tctttttgac tttgcccatc tttcatcttt aaagaaaaaa gtatttgggg
     6421 ttgaccatga tataagccta atttatcaaa ataaaaatga gttcttataa aatatattcg
     6481 cattaattgc aagaaaacat catttaattt ttcttcagaa ttgctattca tacttgccct
     6541 ccttattttt tatattactt agtcag
//
LOCUS       DAGSHT010000186         6513 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGSHT010000186 DAGSHT010000000
VERSION     DAGSHT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.192"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            380..1750
                     /locus_tag="MJB72_003059"
     CDS             380..1750
                     /locus_tag="MJB72_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY8264134.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRVFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGIELIGYL
                     MWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRGSFKRTKKQSAFWYKKVIETDGEILFN
                     DMETHA"
     gene            1776..4166
                     /locus_tag="MJB72_003060"
     CDS             1776..4166
                     /locus_tag="MJB72_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBY8264135.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4168..4839
                     /gene="pgmB"
                     /locus_tag="MJB72_003061"
     CDS             4168..4839
                     /gene="pgmB"
                     /locus_tag="MJB72_003061"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBY8264136.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4878..6281
                     /locus_tag="MJB72_003062"
     CDS             4878..6281
                     /locus_tag="MJB72_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY8264137.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINITGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGEPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKEGIGKLRKCVK"
BASE COUNT         2652 a          668 c         1143 g         2050 t
ORIGIN      
        1 tatatgaaat caattatatt ttcagtattt caacaatgaa aattccattc atttataaag
       61 tctaaatttt agttctcaat acaacctcta tttcgaaata actcctttta tatatataaa
      121 tatacagcaa tatatgagaa taagataaca ataatgagga tataaaaatg tatttgctat
      181 atatataatt atatacatag atatctaata ttgatattta atatgaaagt attgaaaata
      241 gacaatatta attatagtga gtaaaaaata taattggatt tttaaattga attatttaaa
      301 aggtatagta tgaaaaaaat cttataacta aatatgagat agaaattata aaactttata
      361 ctgtgaaaga ggtagatgca tgaaattagg gaaagatttt ttatggggag gggcaatttc
      421 agccaatcaa agtgaaggat tttatttaga tggaggaaaa ggtttatctc agtttgataa
      481 actcccatta aatgataaga gattaaaaga tgtatgttta gatgaggata atattttaaa
      541 tgaaaataat caatactatc caagtcatat agggattaaa ttttgtgaaa attacaaaga
      601 agatatagca atgttagcag aaataggttt taaatgtttt agattttcta tatcttggtc
      661 aagagtattc ccaacaggag aagaaagtga accaaatgaa aagggattac aactttatga
      721 taatataata aaagaattaa aaaaatataa tatagagcca ttaataacga taagccactt
      781 tgatatacct tattctctag ttgaaaaata ttctgggtgg gctgatagaa gagtggttaa
      841 tttatatgta aaatatgctg aaattttgat gaaaagatat aaagatgatg tcaaatactg
      901 gattcctttt aacgaaatga atatgataat gcatattcca tatataggtg ctgggttaac
      961 ttttatagct gatgaaaatc aacttcagaa aaaatatcaa gcagcacacc atcaattatt
     1021 agctaatgca aaaactatag aaataggaag gtatataaat aaagaattta agtttgggtg
     1081 tatgttagct gctggaaaaa catatccata cacatgtaaa ccagaagatg tatttgcaac
     1141 tttagagcat gaaaggcata atttgttttt ttctgatgtt caagttaggg gtgtgtatcc
     1201 aagctttatg aattattatt taaaatcaaa tcaaataaaa ttaataataa aagaggaaga
     1261 tttatcaaca cttaaggata atactgtaga ttttgtatct tttagctatt attctagtgc
     1321 ttgtagttca gctagagctg aaggtttaga aaaatcaaag gcaaacggac cagaaaccat
     1381 aaaaaatcca tatcttccaa aatcaaatag tgtatggcaa aacgaccctg tagggttaag
     1441 aataactatg aatcagttat atgaaaggta tcagttacct ttatttatag tagaaaatgg
     1501 actgggtact caagatattg taaatgaaga tggaatagag cttataggat atcttatgtg
     1561 gggatgtata gatgtaacca gtgtaagtga aggaaagatg acaaaacgat atggcataat
     1621 tcatgttgat tctgatgact atggaagagg ctcatttaaa agaactaaaa aacaaagtgc
     1681 tttttggtat aaaaaagtaa tagaaacaga tggagaaatt ttatttaatg atatggaaac
     1741 tcatgcataa ttcattttaa gaaaggagtg ttattatgag tttaatattt actactaagg
     1801 accaacttat agagcaaggg tttagtaagg atacaataaa atccaatgaa acgctgttta
     1861 cactagcgaa tggacattta ggagttagag ggaatattga agagtctgat tttaataatg
     1921 agtatgtaga taatatgggt acatatgtaa atggatttta tgaagaatct ccaattacat
     1981 atggagaaag cgcttatggc tatgcaaaac aaaatcagac tatctgtaag ttacctaatg
     2041 ctcatatagt aaatttctct attgaaggag attattttga tttatcaaaa ggtattacaa
     2101 gtgaacattc taggatacta gatttaaatg aagggattct taaacgttca tttatatggg
     2161 aaaactcttg tggtagaaaa gttaaagtat ctatagaaag attagtatca caagatatag
     2221 aagaattaat gatgatttca tatacaataa caccattgaa ttttaatgga aaaataaaat
     2281 ttaaaaataa aatggaaaat agtatggaga agtttaattg tgaaaaagta gatgacccaa
     2341 gagtggcatc tataaataag aagcaattta aaattaatgt aaaaaaatat aacaataagt
     2401 attttatgaa tttagttaca gaaagaagta gattagggtt atggtgtggg tttgattcat
     2461 atattacttg tggaaatatt acaaatgaga cctataatgt ttcagatgaa tgtattgagc
     2521 gagaaataga agtctatgcc aaaaaagatg aaaggttaaa tttagaaatt gttgtaggct
     2581 atagtagagt atattctgat ttaaatctaa atgaagaacg tttaaaatca ttagaatgtc
     2641 tggagtcgtt aaaatgttta gaatcagaag cacatttaaa atcattagaa ttggttttaa
     2701 gagaagcatg taatattgga tttgataaag tcaaaaatat tcagagaaag aaaatgaatg
     2761 agttttggac aacctctgat gtagagattg aaggcgataa aactcttcaa ctggggataa
     2821 aaaccaattt atttcatatt tatcaatcag caggaagaga tggaaagact aatatatcag
     2881 caaaaggtct tacaggtgat ggctatgagg ggcattattt ctgggataca gaaatgtata
     2941 tattaccatt ttttatatat actcaaccat ccattgctag agctttatta gattatagat
     3001 actcaatctt agatgcatca agagaaagag ctagagaact tggaataaat aaaggttgct
     3061 tatattcatg gagaactata aatggtaatg aatgcagtgc atattatcca gcaggaacag
     3121 cacagtttca tataaatgca gatatagctt atggtattag aacgtatttt gaagcaacta
     3181 atgatgaaga atttatgatg caaaatggct tagaaattct tatagaaaca gcaagatttt
     3241 gggttgaatt tggagattat atagaatcaa aaaataataa attctgtata aatggagtaa
     3301 caggaccaga tgaatataca gcaatagtaa acaataatta ctacactaat ttaatggcta
     3361 agtataattt aatatatgca gtacagatgg tagataaatt taagcataat ttattttcaa
     3421 atgaagtatt tagaaaagtt aaattacaag aatatgaagt gaaaaactgg ataaatgccg
     3481 ctcaaaatat gtatttacca tatgatgaag agaagaaaat aagcaaacaa gacgattcat
     3541 ttttagaaaa atcagtatgg gattttaaaa atacaccaaa agaaaaatat ccattacttc
     3601 ttcattatca tccattaaca atatatagat atcaagtaaa taaacaagca gatactgtac
     3661 tttcagcgtt gcttttccct gatgagttta gcttagaaca aaagaagaga gattttgaat
     3721 actatgaaaa aataactact catgattcat ctttgtcaag gtcaatattt ggaatgatgg
     3781 caagtgaaat tggaaatcat gaaaaagctt ataactattt tatggacaca gcattaatgg
     3841 atattaataa cctacaaaaa aatacaagtg atggaataca tgctgctaat atgggtggaa
     3901 catggatgag tttagtatat ggatttgcag gaatgaaaat aaaaaatgat aaattaagtt
     3961 ttgaaccaag gatacctaaa cactggaaaa atattaaatt taatatatta tttagagata
     4021 atttagtttc tgtaaatata gaagaatata aatcttatta taagttaatc aaaggtagaa
     4081 atataaccat attacatgat aataaagaaa taaaattatc aaagaacata atagaagtta
     4141 ataattgttt taaagggagt aattaatatg aaagcagtct tatttgatct tgatggagtt
     4201 ataacagata ctgctaagta tcattttgaa gcttggcatg agatagcaaa atcgctagat
     4261 atagaaatag atgaaacttt taatgaaaaa ttaaaaggca taagcaggga agaatcatta
     4321 gataaaatat tgttatctgc taataaagta gatagttata ctaaagagga aaagttaaag
     4381 gtgtgtgaag aaaaaaataa catttatata aagttaataa actcattaac atataaagat
     4441 atattaccag ggataaatga gcttataaaa gaacttaaaa aaaataatgt gaaattggga
     4501 atagcatctg ctagtaagaa tgcacctagg atattagaat ctttgggaat aaaagatgaa
     4561 tttgattgta tagtaaatcc atctttatta aaaaggggaa aaccatatcc tgatattttt
     4621 attgaaggga gcaagattct aaatttaaaa caatatgatt gtgtaggaat agaagattct
     4681 tattcaggta ttaaagctat aaatgctgct aatatgacat caattggtgt ggggaaaaaa
     4741 gaagtactag gaaaatgtga ccatattgta gaagatacta gcttattaaa ttacaataca
     4801 ctattacaag cttggagtaa aaagattaat ttggattaaa attatattaa aaattatttt
     4861 aaaagagagg ataaggtatg aagaataaat atgaagaagt agctcaattg ataattgatg
     4921 gccttggagg aaaggataat ataattgact taactcattg tatgacacga ttaaggttca
     4981 tcttaaaaga tgaaaagaaa gtcaatgcag ataaattaaa atctatagat aaggtagttg
     5041 gagtaaactc aacttcaact caatatcagg ttataatagg taatgaggta ggtgttgtat
     5101 ttaatgcaat aataaataag gggataaata tcacaggaga tattaaaagc gatataaaaa
     5161 ataaaaataa aaaaaatagt attttaaata atataataga tacaataact ggttgtatga
     5221 cacctatgat accagccctt acagcatctg ggatgataaa agttatatta tctctttgta
     5281 ccacttttaa ttggctatca tcaaattcaa gtacatatag agtattagat ataataggag
     5341 atgtagcatt ttactttatg cctataatac ttgcaataaa tgcagctaaa aaatttaatg
     5401 ttaatacatc aatagcagtt atagtagtag gtgtgttttt acatccaaat ttctctgcat
     5461 gggtatcaag tggggaacct atatcattta taggagtacc aattcaagat gttatatatg
     5521 cagcatctgt tatccctgca cttttaacag tatggatgat gtcatatata gaaaaattca
     5581 ttgataaatt aacacctagc atgttaaaaa caattttaaa tccaacttta gtattattaa
     5641 taagtgcgcc aatagcatta atagtaattg gaccaatagg aaatttctta ggagagggat
     5701 tagccagtat aataaattta ctacaaggaa ggttaggatt tataatggtt tgtttattag
     5761 cagctgcaat gccatttatc gttatgacag ggatgcatca tgcattgact cctatattca
     5821 tatcagcatt tgcaactaca ggacaagaat cgctaatatt aatatctcaa gtttgtgcta
     5881 atttagcgca aggtggcgcg acattagctg tagcattaag aagcaaagat agtaagatga
     5941 aacagttagc atcagctgca tggatatcag ctatcatggg tataactgaa ccagctcttt
     6001 atggagttac attgaaactt aaaaaacctg ctatgttagc atctatagga gcaggaatcg
     6061 cagggttatt tgcaggaatt actcatgtga ctttatatgt tccacaaaat agtataatgg
     6121 cagtacttgg attatcaggg gaaaagggga tttcaaatat agtaaatgga cttatagtta
     6181 tatttatagc attggttgct ccatttatta tgacatatgt ttttggagtt gaaaaaaatg
     6241 ttaaggaagg tataggtaaa ttaaggaagt gtgttaaata gtatgagata aaaaaattca
     6301 tgataaatat ttataataaa agtgattaac ctataaaact tagtaaataa tagttatata
     6361 ataattaaga taaactcaat ttaaaacttt aaaaaatacg attcttaaat agtcaaactt
     6421 tagtaaaata tataaaaata tgttttgtta aggtttggca tattttttat tttggagaag
     6481 aaatttgata cctatattcc tactacagga aga
//
LOCUS       DAGSHT010000187         6427 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGSHT010000187 DAGSHT010000000
VERSION     DAGSHT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.193"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            107..553
                     /locus_tag="MJB72_003063"
     CDS             107..553
                     /locus_tag="MJB72_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264138.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            823..1275
                     /gene="accB"
                     /locus_tag="MJB72_003064"
     CDS             823..1275
                     /gene="accB"
                     /locus_tag="MJB72_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBY8264139.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            1291..2652
                     /gene="accC"
                     /locus_tag="MJB72_003065"
     CDS             1291..2652
                     /gene="accC"
                     /locus_tag="MJB72_003065"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBY8264140.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            2667..3521
                     /locus_tag="MJB72_003066"
     CDS             2667..3521
                     /locus_tag="MJB72_003066"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBY8264141.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            3542..4486
                     /locus_tag="MJB72_003067"
     CDS             3542..4486
                     /locus_tag="MJB72_003067"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBY8264142.1"
                     /translation="MIENNSEKIKILENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            4675..4935
                     /locus_tag="MJB72_003068"
     CDS             4675..4935
                     /locus_tag="MJB72_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBY8264143.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            5088..5459
                     /locus_tag="MJB72_003069"
     CDS             5088..5459
                     /locus_tag="MJB72_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY8264144.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            5489..6334
                     /locus_tag="MJB72_003070"
     CDS             5489..6334
                     /locus_tag="MJB72_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264145.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         2482 a          671 c         1103 g         2171 t
ORIGIN      
        1 aacataaatt aatgaaatta acaaaatttt attcagaaac atataaagat atattttact
       61 atttaaatgc atatcattat atatacagtt ttaggaggtg aggtatatgg aatctaaatt
      121 ttttaattgg tacactcaat ccttaggggg aacttttggc attatatcat gtatatgcgc
      181 atatttaaat ggatacatgt ttgtttatac aaatagtgct agcaaatatt ttgaaagtat
      241 aggatttttg ggaataattt caagttatac actacttcca ctttgtatgc ttactttgat
      301 ttttggagtt atatattctt atactccaga aacatccgac aaaagtattt ttaatatacc
      361 tttttttact ttaaataaaa gattaatact tattacagta gtttttggtt ttataggtgc
      421 tagagtttac tttataattc ctgcaattct aataactttt aacctatata gcaatgtttt
      481 gatagctaaa ttaaagtaca tcctatgtaa aaataaagaa aatgatacta atttgaaaga
      541 aagcgaagtc taaattgcat aatattggta tttgtcttaa aaattcccga ggtattaatt
      601 tgtattctct tgtattttgc aaaaaatatt gaattttatt cattttatac ttataattta
      661 tattaataat ggaaaattaa aacaaatata agttactggt tgttaattta aaattaaaag
      721 atatattgat gtgttgattc tctagtcagc actttttctt ttataaattt gttttttgtg
      781 atataatatt agcaccaggt aataatacaa gggggtaaca aaatgaacat caatgaaatt
      841 aaggaattat taaaggccat agactcaact aacttagaat atgtaaaact tgaaagtagc
      901 gatttaagat tagaagtatc taaaaaagct caatctacaa gttcacctgt tttaagtgta
      961 caacaagaat cagtagtgga tctttcttta gaaaagccag ttgtaaacga tacacctgtc
     1021 acatctaatg agaatctatc agtagtagta gctcctttga tgggaacatt ctatgattct
     1081 ccaagtccag atgcagatag ctttgttaaa gttggcgatg tagttgaaga aggcgacact
     1141 ttatgtatat tagaagcaat gaagcttatg aatgagataa caagtgaaat taaaggtgaa
     1201 ataattgaag tattagttag taatgaagaa ctagtagaat ataatcaacc tttatttaag
     1261 ataaaaccat tgtagtagga ggtatttaag ttgataaaaa aaatactagt tgcaaataga
     1321 ggcgatatag cagttagaat catcagaaca tgtaaagaac taggtataaa aactgttgct
     1381 atctattctg aaatagataa agattgtttt catagatata tagctgatga atctatttgt
     1441 ataggaccaa ataatataag taagagttat aataatatag aaaatataat ctatcttgct
     1501 cttaaattaa agtgtgatgc tattcatcca ggctttggat ttttatcaga gaatcctgaa
     1561 tttgcaaagc aatgtgaaga taataatatt atttttatag gtccaacaag agaacaaatg
     1621 attttgatgg gagataaatc tagagcaaga gaaactatga tggaattgaa tatccctgtt
     1681 gttcctggtt cagaatctgt tttgaaaact aaggaagaag ctttagaagt tgcaagagaa
     1741 attggatatc ctgttatgat aaaagcctca agtggaggcg gtggaaaagg tatgagaatc
     1801 gttagaaaag aagaagaact tttttcaaat tttgatatgg caagttcaga agctctagca
     1861 gcattttcta atagtgattt atatatggaa aagtttatag aaaatcctag acatatagaa
     1921 gtacaagtat ttggagataa acattcaaat gcaatacatt taggtgatag agattgttct
     1981 atgcaaaggc gaaatcaaaa agtaatagaa gaatctttaa gtccatacct ttcagatgaa
     2041 gaaagacaaa aacttcataa aattgcagta gatatagtaa aaggagtagg ttatatcggt
     2101 gcaggtacta ttgagtttat agttgacaaa gataaaaact tttattttat agagatgaat
     2161 acaagaatac aagttgaaca tccagttaca gagatggtaa caaatcttga ccttataaaa
     2221 cttcaaatat ctatagctaa tggggataaa attcctttta aacaagaaga tattactttt
     2281 agaggtcatg ctattgaatg tagaataaat gcagaagatt catcaaagaa ttttgctcca
     2341 tcaccaggta agatagaatc attaaattta cctggaggtt ttggtgttag atttgatact
     2401 tttgtatatg caggatatac tataccacct ttatatgatt ctatgatagg taagttaata
     2461 tgttgggctg aaactagaga agaatgtata agtagaatat atagagctct agacgaaatt
     2521 attgtagaag gtattaatac aaatgtagaa tttcaaaagg cacttgtaac aagtgaggag
     2581 tttagagaag atactcatca taccaaattt atagaagatg tctttatgaa gaaagaattt
     2641 gctacattat aaggagtaaa tagattatga taaaaaaatt tttatcatca aaagaatcta
     2701 agtatgttac gatttcttta gatgacaact ttaaaaagaa tagtgtagat gataaatttt
     2761 ggacctattg caaaggttgt gatagtcatg tatttagaaa ggacatagaa gaaaattcat
     2821 ttgtatgtcc aaaatgttca agacattatg gcttaagagc aagaaaaaga ataaatttat
     2881 tgattgataa aggaactttt atggagttta attctgatgt agaatttcaa aatccactaa
     2941 attttcctaa atacaaagaa aaagtagact cttataagga aaaaacaaaa gagtcagaag
     3001 ctgttgtcac aggttatgga agaataaatg gaattaaaac agttatttgt gtaatgaatc
     3061 ctgattttat gatgggaagt atgggttcta ttgttgggga aaaaataact tattcaatag
     3121 agtatgctgc tgaaaataat ttacctataa ttatttgttc tgcttcagga ggagctagaa
     3181 tgcaagaagg tatggtatct cttatgcaga tggcaaagac ttctcaagca ctttctaagc
     3241 ttgaagaaaa atcattgcca tatatatcag ttctaactga ccctactact ggaggagtaa
     3301 ctgctagttt tgctatgctt ggtgatatta taatttctga gccaaatacg ttgataggtt
     3361 ttgcaggtcc aagagtaata gagcaaacaa taaatcaaaa acttcctgaa ggatttcaaa
     3421 cttctgagtt tttacttgaa aaaggtttta ttgacatgat agtagataga agaaaaatga
     3481 aggaagtatt atatcaaatt cttgctatgc acaagaaata gggcataaag gaggaactgt
     3541 tttgattgaa aataatagtg aaaaaataaa aatcttagaa aatgatatta aacaattaat
     3601 aactatttct aaggcaaata atatagattt aagtgataaa ataaattcac ttaatgagaa
     3661 attggaaaag cttaaggaag atgctttttc acatctatca gcatatgaaa aggtaacact
     3721 aagtagagat ataaagagac ctacgacatt ggaatacata gaacatattt gttctaattt
     3781 tttagaactt catggagata ggctttttaa agatgaccct tctatggttg gaggaattgg
     3841 tcagatagga aagtttaatg ttactatagt tggacaccaa aaaggaagag atacaaaaga
     3901 aaatataaaa agaaattttg gaatgcctca tccagaagga tacagaaaag cattaagact
     3961 tatgaaacaa gctgaaaaat ttaatagacc tataataacg tttattgata catcaggagc
     4021 tttttgtggt ctagaagcag aagaaagagg acaaggagaa gctattgcaa gaaacttact
     4081 tgaaatgagt aaactttctg ttccagtaat cacttttgta attggtgaag gtggtagtgg
     4141 aggagcttta ggtatcggag taggaaatga tgtatgtatg ttagaacatt cagtttattc
     4201 tgtaatttct ccagaagggc tttctagtat tctatttaaa gattcatcta aagctaaaga
     4261 agcttgtgat gttatgaagc ttacaagtaa tgatttatat gatttaaaaa taatagataa
     4321 gattataaaa gaacctcttg gaggagcaca aaaagatgta gaagctgttt caaaagaaat
     4381 aaaagcttat attttagaaa gattaaatca ttacaaagat atggataaag aggaaattat
     4441 agctcaaaga tataaaaaat ttagaaatat tggaaaatgc ttatagaata taaagaaatt
     4501 tgtagttttt tcataataaa atggttaaaa aaaataaagg ttttcttaaa acgattatag
     4561 aagtatattt ttataaaagt atactttaaa tgatgattgt aattagtaaa tggcaattat
     4621 gatttagtac tttaggtaat ggatacttta acaaattaac aaggagatgt tgaaatggag
     4681 gtactaaaag tttcatcaaa atctaatcct aattctgtag ctggagcctt ggctggagta
     4741 ctaagagaac gaggcgttgc agaaatacaa gctataggag ctggagctct aaatcaagcg
     4801 ataaaatcaa ttgcaatagc aagaggtttt gttgcaccta gtggaatgga tttagtttgc
     4861 attcccgcat ttactgatat agaaatcgaa ggtgataaga aaacagccat taaacttata
     4921 attgaaccta gataatatat ctctttatta gaatttaatt aagttttaaa ggttggctat
     4981 agaaatagga cactatagtg aacctttttt actttatatg ttttatttta tgtaactttt
     5041 aatgtataat cttaagtaaa caaataagat tggaggaaat atacattatg aaattaaaat
     5101 tattggtaga agagtatgca gtatgtagat taaataatga cagtaaaata ccaacatgga
     5161 tagatacaga gaagttttac tctataacta gaactgatga tgaattaagt gttgtatgtt
     5221 taaataataa tattccttct gatgtcaagt cagaaaagga gtggagaata cttaagatac
     5281 taggtccttt agacttttca cttgtaggaa ttttatctaa gttaagtagt ttgttagcag
     5341 acaacaaaat aagcatattt gcaatttcta cgtatgatac agactatatc ttagtaaaag
     5401 aaaaagatat agaaaatgct tgtaaaattt taagctgtaa tggatatgaa gtagagtagt
     5461 taaatacttt tacatgagga cttaacttat gatttatata atcgaaattt taaaaataag
     5521 tgtaataaat attttttgtt tcataggaat atttattgta tttggattga tttttagcat
     5581 tacagaaaat ctaaataata aatttatatt ttcatctttt ggaaaaactg gaattattgt
     5641 aactggagct ataggaacat ttgttcatga attgagtcat ttgataatgt gtttaatttt
     5701 catgcataaa ataaattcag ttaagttttt cagaccaata gaaagtaaaa atgatggtat
     5761 actaggatat gtaagtcata gctataaaaa agataatctt tatcaaagta taggaaattt
     5821 ctttatagga attgcaccta ttataggtgg aacaataatg attattttaa tttttaagat
     5881 attactgcca gatagttata tgaaagtgac tcaaaatata gatttaaagt tatatgtaag
     5941 tatgattaat aacttaaata taaatggttt tatacacatt attttaggtg atatttccaa
     6001 ttttataaga ataatgttgt taacacctag tgtatattca ttgaagtatt ttatatttat
     6061 gtttttaatg tattctatat ctacacatat gtcacttagt agtgctgatt taaaaaatag
     6121 cttaaatggt ctgagcttta tagttatagt tatttttgta ataagttcaa ttacatactt
     6181 atttggattt gataacttga atatatccag tttaatagtt aagtataaca tatttataag
     6241 tttctttatg gcaatagggt ttattttttc aatagtaact ctcataatat cctatatttt
     6301 aagcttagtc agcccattta aaaactttag ttaaataaat acatatttta ctaatatttg
     6361 gtttcgttag aaacatataa aaatattata taaaaaagat tttagtagag cataaaaaat
     6421 ttctcaa
//
LOCUS       DAGSHT010000188         6335 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGSHT010000188 DAGSHT010000000
VERSION     DAGSHT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.194"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(22..1620)
                     /locus_tag="MJB72_003071"
     CDS             complement(22..1620)
                     /locus_tag="MJB72_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8264146.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            1948..2124
                     /locus_tag="MJB72_003072"
     CDS             1948..2124
                     /locus_tag="MJB72_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264147.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            2298..3428
                     /locus_tag="MJB72_003073"
     CDS             2298..3428
                     /locus_tag="MJB72_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY8264148.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKSYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            3669..4019
                     /locus_tag="MJB72_003074"
     CDS             3669..4019
                     /locus_tag="MJB72_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY8264149.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            4027..4476
                     /locus_tag="MJB72_003075"
     CDS             4027..4476
                     /locus_tag="MJB72_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBY8264150.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            4578..5153
                     /locus_tag="MJB72_003076"
     CDS             4578..5153
                     /locus_tag="MJB72_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY8264151.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            5146..>6335
                     /locus_tag="MJB72_003077"
     CDS             5146..>6335
                     /locus_tag="MJB72_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBY8264152.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHG"
BASE COUNT         2576 a          789 c          928 g         2042 t
ORIGIN      
        1 gctatagcta aaaaagtgca actaattgct taaagaaaca gaattatcca cttcagcacc
       61 catataaaca atattaagat ttcctaaact gctatcccaa acgatactat taataatagt
      121 agataataac aatctcttat catctaaact ggcattatca acttcatcat taaatctctt
      181 caaattacta acaataatat caatattaat actgtcacta ctaatattga tgttactaga
      241 ttccaaaata ttaatcttat cctttaaaat actaatctca ttatttaggc tctcaatctg
      301 ggaaactata taatcagaag cagtactacc agtatttttt gcaagctcta taactaaatt
      361 tttaataagt ctttccttat ctaaaatttg atttttaagt aaattagtct cagatttaaa
      421 aatattaaaa acattatcag tcttaatgtt attactattg aatgcactta taatagactc
      481 ttctttacaa ttcttaattt tatctataac taaactttca actaatggac cattaagatt
      541 tttacagttg caactatcag acccaagtgt ttttttagta ccacaaatat aataatactt
      601 taaatccttg tcagtttttt tgcctttata ggtaattctc atattagaac cacatttacc
      661 acattttata agaccagata aaagagcagt ttttccagtt gatatccttg gagttttatc
      721 acgattttta tctaattgat tttgtacttc aagccatatt tcaggttcta tgactccatg
      781 gtgttttgca attgctgcta ttgagttaga tgtattttta gcataagtaa ggattccaca
      841 ttttctatct ggagtaccta caacatccat accactattt ttaaggtaat ctaaaatttc
      901 atcattagct tttacatatg ctggatttct aagtattcta gataaagaac tagggtcaaa
      961 tttaccacca cgaggacccc ttattccatt attatagaca tatttatata acttagataa
     1021 ggacctaagt tctaaatatt tattgtatat tagctttaca atctccattt gttcatcatt
     1081 tacttgaagc ttatacatac ttctttcttt tagattttca tctaagaatg tgattttttc
     1141 actgttgaat ccatgaggtg tattgcctcc aagccatcta ccactacggg ctaattcata
     1201 catattgtct tttatacgtt ctgctattgt ttctctttct aattgagcaa atacacttga
     1261 tataaacatc atagctcttc ccattggagt tgaagtatca aattgttctt tgatagatat
     1321 aaatgatatg tttagttcat ttaatttttc tattaaagta gagaagtcag atacatttct
     1381 cgatatccta tctaatctat aacaaactag gtaactaaat ttcttatctt tggcatcctg
     1441 catcattttc ttaaattgtg gtctatctat attgctacca ctaaatccct catcttcata
     1501 tatcaaaaaa tcttttacac caatagactt tgcataatca atacataatt gtatttgatt
     1561 ttctatagaa tcaccttttc cagtaaatct tgattttctt gaataaattg cagctttcaa
     1621 aattaaaacc ccctataagt taggatatat atttgaaatt atttatatac aaccttttaa
     1681 gtaaacttat tagaatgaat ttattaatta ggtaaattta tataattcta ctttttataa
     1741 tatatgacta tttattttat ttctttacta ttcaagttct aaatcaataa taaattttga
     1801 tatttaattg tatcaggaca aatctctaat ttcatagatt attaatataa aatcataggt
     1861 tagtgtgaaa aagttgaaat ttaaatattt taaaagataa tttttttgaa ctaataggta
     1921 taacaaaaat ttataggggg attgcatatg gaaatcaatg ttatagaaat tttccctaaa
     1981 gataaagcta aacttaataa aatagaaatg gataaagcta gttggtttgt aaatataata
     2041 agtaaaaaat atcctaaaga agctttaaat gaagcattta gtactttaga aaaagaatta
     2101 aatataagta aagctaatac ataagctttg attattgatt ttatatacta ttggttttat
     2161 acgctatgac ttgacattat attaatcaaa tagtatattt ttaaatatag cacaatgaag
     2221 tgttaaaaga gcaatggagc tccatagata aagtctatgg agcttttttg cgtatttaac
     2281 ttaaggagga taggactttg aataactttg agattaaaac tagaataaaa tttggagaag
     2341 gctcactaaa agctttgcaa gaattaaaaa acaaaaatgt actaataata acagaccctt
     2401 ttatggtgaa atcaaaaaca atagataaaa taacatctaa tttaataaac tcaacatatc
     2461 aagtttttga agaaatagta ccagacccac caatagaagt agtagtagca ggaatagaag
     2521 catttaaatc tataaatcca gacgtaataa tagcattagg tggcggttct gcaatagatg
     2581 cagcaaaatc tataatggac ttttctaaaa aaatattaaa aattaacgaa gtagagttta
     2641 tagcaatacc aacaacaagt ggaacaggtt cagaagtaac atcatttgct gtaataacag
     2701 atagtcaaaa aaaatgtaag tatccattag tgagtgatga tttacttcca gatatggcaa
     2761 tactagaccc agagcttgta aaaactgtac caaattttat aacagcagat acagggatgg
     2821 atgtattgac acatgctata gaagcttacg tatctacaaa ttctaatgac ttttcagatg
     2881 cactagctga aaaagctata aagttagtat ttaaatactt aataaaatca tataaagatg
     2941 gaaatgacat agaagctaga gaaaaaatgc acaatgcatc ttgtttagca ggtatggcat
     3001 ttaaccaagc atcacttggc ataaatcatt ctattgcaca tgtattaggt ggaaaattcc
     3061 atataccaca tggaagaaca aactctatat tacttcctca tgtaatagaa tataattcat
     3121 caatagtagg atatactaac acaacttact ctgatacagc taaaaaatat gcacaaatag
     3181 cagaattagt aggacttggg agtgctaata taagaatatc tgttagaaat ttagtaaatg
     3241 aaataaagaa actacaaaaa gaaatgaata tgccaactaa attgacacaa tgcaaattaa
     3301 gtttagaaga tataactaat gaagaaagag aaatagcaaa acttgctata aaagatactt
     3361 gtacattaac taatcctaag gtaccaactg aagatgatat aataaacata ttaaagaaca
     3421 ttaaataact acattttatt acatgtaaat ataatattat acaaataggg aaaatgtttt
     3481 taaaaatata aattatatat tgacaaatat aaaacataaa actataatga aagtatcaca
     3541 aaggcgtgat actttagtag aaagcaatgg agcttcttag gaatattaat attattctta
     3601 tggagctcct tttattttaa tcaaatagct ttttaataaa ctatattcaa agaaagcagg
     3661 gaataaattt gactgaagaa tctaagcaaa gagttattca agaatatgtt cctggaaaac
     3721 aggttacatt agcacatatc atagctaacc ctaatgaaga catatataag aagttaggat
     3781 tagtacttga taaaaaagat gcaataggaa tacttacaat aacaccaagt gaagcgtcaa
     3841 taatagcagc agatgttgct acaaaggctt caaatgtatc tttaggtttt atagatagat
     3901 ttagtggttc agtagtaata tcaggtgatg taagttctgt agaatcagcg ttaaatgatg
     3961 ttttagaagt cttaggaaat atgttaaact tctcatctac aaaaatcaca aggacataat
     4021 aatacaatga agaatataat atttatggga aagacaggta gtggtaaaac taccttatgt
     4081 caaaaactag accagttaga attaaagtat aaaaaaactc aatcagtgga actttataat
     4141 aatgcaatag acactcctgg tgagtatatg gaaaatagaa acctatataa tgccttaata
     4201 acaacatcag cagatgctaa aattatagca atagtgtatg atgccacaca agaagaaaat
     4261 tatatagcac caggttttgc cagtatgttt tgcaaagacg taataggaat tataactaaa
     4321 ataaataaag ctactgaaaa cgaagtcaat atagcttatg aaagactatc actagcagga
     4381 gcaagtcaaa tctttaaaat agacacagtt gataatattg gacttgaaaa attatttaaa
     4441 tatctagaaa aatataaaaa tatagatgaa gtttaagggg aaatattttt ttattcaaaa
     4501 atattattaa agtatgataa aataaaaaaa tgtataaact attttaagaa gatttaatct
     4561 ttaagcgagg taggaacatg gggaaaaaaa tactattggt tgatgatgaa ccactgttaa
     4621 ggatggatac aaaagatata ttagaaagtc atggctatga aataataggc gaagcaagtg
     4681 atggatttga agctgttgaa atgtgtaaaa aacacaaacc tgatttagtt ataatggata
     4741 tagagatgcc aatacttgat ggtataaagg caggcaaaat aatatgtaaa gaaaatctag
     4801 ctggtggagt acttttatta acttcatttg aagataaaga atacgtagaa aaggctaaaa
     4861 gtataggtgc ttatggatat ttagtaaaaa cagcaagtga aaaatcactt atcccaacca
     4921 tagaaatgtg tttaaataag gtaaatgaat ttgaaaaaat gaaaaaagat ttagaaaaag
     4981 taaattctaa attgaatgaa agaaaattag tagaaagagc aaaaggtata cttataagag
     5041 agtttaagat ttctgaggat gaagcataca ctagaataag aaaattaagt atggataaaa
     5101 gaactacaat gatggaaatt gccaaaatga ttataatagg atacgatgac taatacgata
     5161 aaaaaaatat caactgcaca aacatactta aataataacg aaattgaaaa aattatagaa
     5221 gtatcaaaat ccttagattt aatggcttct ttttatgaag ctgatgtttt tatagatgta
     5281 ctaagtaaat ttgatgatga agcaattgtt gtggcacatg gtagacctaa agaaaagtca
     5341 atgtactgtg aaaatgttat aggtaaaaag gctttaattg aaaatgaacc aggagttata
     5401 aagtgcttaa aaacaggaga aagagtaaga gatattaaag ctttaacaca ggaatataaa
     5461 ctggtcaaac aaaatattca gccaataaca ctagatgaca aagttttagc agtactaata
     5521 gttgaaaaag atataagtag tgaagttaag aatgagttta atgctagtga agacgaagaa
     5581 aataaaaatt tacatcagtt tataaaactt attggaaata atgacttttt agttgataat
     5641 ttaaattcag ccatattaat atttgataaa aatggaaaat taaaaattaa aaatcgtatt
     5701 gcaactgaga tatacaaaaa tcttgggttt ggatatgata tacaagatat gcattacaat
     5761 gatttagata tagaaaccaa cacctttgaa aatatattaa tggatgaaaa ttatgatgag
     5821 acaaatgaag tttcaatagg taatatgttt tttaaaataa agaccatata catcaaagat
     5881 gatgaattta aggtttgcaa aatagtacaa gatataagtg atttaaagag aaaagaagaa
     5941 gaattagttt tgaaaacagt ggctataaga gaagctcatc atagagtaaa aaataatctt
     6001 catacagtaa tatctataat aaaaaaacaa agtagattat ctcaaaatga tgaagtaaag
     6061 cagtgtttag ataacataat aaatagggta tttgcaatac tttcgactca ttatttacta
     6121 tctaaagaag ttgataataa tatatctata cgagaaggta tcaatttatt agtgtcaaat
     6181 attcaaggtg gctatttatt tagtaaggat atagatatac gtataactgg tgaagatttt
     6241 aaaattagtg gagaaaaagc aacagcaata cttttggtta taaatgaaat actacaaaat
     6301 tgttatgacc atgcattttc ggatagagaa catgg
//
LOCUS       DAGSHT010000189         6289 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGSHT010000189 DAGSHT010000000
VERSION     DAGSHT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.195"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            124..909
                     /locus_tag="MJB72_003078"
     CDS             124..909
                     /locus_tag="MJB72_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY8264153.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1188..3098
                     /gene="phnW"
                     /locus_tag="MJB72_003079"
     CDS             1188..3098
                     /gene="phnW"
                     /locus_tag="MJB72_003079"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBY8264154.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3457..4005
                     /locus_tag="MJB72_003080"
                     /pseudo
     CDS             3457..4005
                     /locus_tag="MJB72_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4107..4316
                     /locus_tag="MJB72_003081"
                     /pseudo
     CDS             4107..4316
                     /locus_tag="MJB72_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            4885..5292
                     /gene="tnpA"
                     /locus_tag="MJB72_003082"
     CDS             4885..5292
                     /gene="tnpA"
                     /locus_tag="MJB72_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBY8264155.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            5294..>6289
                     /locus_tag="MJB72_003083"
     CDS             5294..>6289
                     /locus_tag="MJB72_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8264156.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKVRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTNVDIEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEM"
BASE COUNT         2618 a          629 c         1143 g         1899 t
ORIGIN      
        1 caaagtcttt tgaaaataac tttaattaag taaaaaattt aatacataaa tatgatttca
       61 taaatcttag gtatcattgt aatattatag gagaaaaata tattatattt aggaggaaaa
      121 tgaatgtctt taaattcgat agatagaaaa aaagaaatac ttaaattatt agattcaaaa
      181 gggaaggtaa acactaaaga attagtaaaa agattagaag tatcatcaga aacaataaga
      241 agatatttgg aagaattaga aaatgaaaat aaattaaaaa aggtatatgg tggtgctata
      301 aaaataaatt atggagtgat tgaaccagaa catataaaga gaaacaatac taacctagat
      361 aaaaagcaaa gtattgctaa atatgcagct aagtttgtaa atgacaatga gtcaattatc
      421 atagatgaag gtactaccaa tttgcagatt gtagattata taatatctgt aaacaattta
      481 aaaattatta caaactctta tccagttcta tcaaaattga tttcttatga gaatcaaggt
      541 ttgtttgatg gagaagttat atttattggt ggaaaagtaa atttaaaaca ccaaaggaca
      601 gcatgtccaa tgtcaataga tactatgaaa aacttatacg tagataaagc ttttatatct
      661 agtgaaggta tacttgatag ttttggaatt agtagtatag accaaaataa ggcattgttg
      721 tcaaaagaat atataaaaaa ttcaaaggaa agttttgtat tatgtgatag ttctaaaata
      781 ggaattgcta gtatgtataa gatagcagat ttagaggaat tagattttgt tatttgtgat
      841 gtagaacctc cgaatgaatg gaaggataag ttaaatgaga taaatataac atggatagaa
      901 gcaaaataaa cccttagttt tatataatac aactatgctc aatacaacta aattcataaa
      961 atatagtcag ttcatagatt aagattagaa agtaaatttt agattgtaac ttaggatttt
     1021 atgggaatat gatacaaaat aaaaacaact aaatacaact aaaaacagaa atttaatata
     1081 tatttaacct aaatatgatg tataattaac atatgaaagt tatactttaa aagtaaaaac
     1141 aattaaaaac acaaaaattc taaaaagaat ctatggaggc tataaatatg aaaaagatat
     1201 atggagaaaa aataaaagca gtagtattcg attgggctgg aacaacagta gattatggat
     1261 gttttgcacc tcttaatgta tttatagaga tatttaaaag aagaggtata gatgtaacaa
     1321 tggaagaagc aagaaaacct atgggaaaat taaaaataga ccatataaga gaaatgtgtg
     1381 aaatggatag aataaagaat atttggtcag ataagtttgg taaagtgcca actgaagatg
     1441 atgtaaatga actttatgct gaatttgagc caatgttatt tgagacatta gaagagtata
     1501 cgactccaat tcctcatgtt gttgaaacta ttgaaaagtt gagaaaaaat ggattaaaaa
     1561 taggttctac aacgggttat acaagagaga tgatgaacat agttgaacca aatgcagcaa
     1621 agaagggata ctcacccgat tttcttgtaa caccatcaga agtatctcaa ggaagaccgt
     1681 atccttggat gtgctataaa aatgctgaag ctttaggggt gtctcctatg agttctatgg
     1741 taaaagtagg agatacaata agtgatgtaa aagaaggcgt aaatgcagga atgtggagtg
     1801 ttgctgtaat aaaaggttca agtgagttag ggcttacaca agaagaagta gagaacatgg
     1861 ataaggaaga attaaaagct aagatgagta tagtttcaaa gaaatttaaa gaggcaggtg
     1921 cacattttgt aatagaaact atggctgagt tagaagatat actaataaaa atagagaatg
     1981 aaactattaa atcagatttt gtaccagaaa atgattacat attacttaca ccaggaccac
     2041 tatctactac taaatcagta agagcatcta tgttaaaaga ttggtgtact tgggatgttg
     2101 aatataataa cctagtacaa gatgttagaa gaagattagt atctctagca acccaaaata
     2161 cagataaata cacaagtgta ttaatgcaag gaagtgggac tttttcagta gaagctataa
     2221 taggaagtac tatttcaaaa gatggtaaac ttcttgtaat tgcaaatgga gcatatggaa
     2281 agagaatgaa agatatatgt aattatctta acatacaatt tgttgactgc acatttaaag
     2341 atatagaagc agtggattta aatgtagtag aaaatttatt aaaagaaaac aaggatataa
     2401 cacatatatc aatggttcac tgtgaaacta ctacaggtag attaaatcct atacaagagg
     2461 tagggaaact tgcaaaagaa tataacaaaa tttatatagt tgatgctatg agtagttttg
     2521 ggggaattga gattgatgtt gaagatttta atatagattt tttagttagt agttctaaca
     2581 aatgtataca aggagtacct ggatttggtt ttataatagc taataaagag aaattatcta
     2641 aatgtaaagg tatagctaaa tcattatctc ttgatgtgta tgcgcaatgg gagactatgg
     2701 agaaaaataa tggtaaatgg agatttactt cacctacaca tgttgtaaga gctttttatc
     2761 aagctttatt agaattggaa gaagaaggtt ctgttgaaaa aagatatgct agatataaag
     2821 aaaatcagtt tacaatagct tcaagattaa aatctttagg ctttgatact ttggtgaatg
     2881 ataatgctca atcacctgta ataacaactt tcttatatcc taaaaatgca aagtttgaat
     2941 ttatggaatt ttatacttat ttaaaagata atggatttgt tatctatcca ggtaaattga
     3001 ctgatataga tacatttaga ataggtagca taggtgaagt ctatccaact gatatggaga
     3061 gactagctga tgtaatagaa aaatttatca atagatagaa gaagatagtg tcttgtaggt
     3121 tacagaatag tgcgcaaagc ctactccttt aggtgtgtga gatgatagca caaacagtgt
     3181 ataaaaagta ttgacagtag aatttaaaga cttatatttt agaagtttat acaatgaata
     3241 tataaacatt gagtaatagg gatatatttt atttaaacca caatctaaga aattagattt
     3301 atagtttaaa taaagcattt ccctattatt tttatgccta tttatagaaa ataaatgata
     3361 gttgctttat taagtattat tatcatactt gacaacgtaa caatgttttg ttatattagg
     3421 tttaaagata aaatacggta aagaaaggat atcattatga atgaagaatt aagttcacta
     3481 agatttgatg gtgttggaaa atgtaaaggt ggaatttttg ataatgttaa tatatcagga
     3541 acaggcaaaa tagaaggaag tgtaaaatgt agtaaatttg attcttctgg ttcagcaaaa
     3601 gtttttggga caacagaatg taatgaattt tttacatctg gtttaagtaa agtagaagga
     3661 aacatactgg catcaaaagt tgagatatcg ggacttctta gatgtacagg aaatattaat
     3721 tctgttgaaa ttgatactag tggaattatt aatgtagaag gaagtataaa atcggatatt
     3781 attcatggag aaggatattt aaaagcaaat gagaatatag attgtgaaaa tatcaattta
     3841 agtggaatct tggagtgtaa tggatttttg aattgtgaag aagttaatat tagtttaaat
     3901 ggaacttcta attttaatga agttggagca agtagtataa atataaaaaa ggggcatgga
     3961 gattattgca aaaaatttcg tatgctagaa aaaaagcata tctctagtag ataattatga
     4021 tgttggaaat attgaataca gtgataattt atcagtttca gataattcta ctgtaaaaaa
     4081 cagtataaag gtgggctaga agagctatgg aagaattaat atctaagaaa gatttattga
     4141 gcaaaactaa tatatcttat ggacaattat atagatggaa aagaaaaaac ataataccag
     4201 aggaatggtt tataaaaaaa tcagctttta caggtcaaga gactttcttt ccaagagata
     4261 aaatactaga aagaatagag ttaatattgt ctatgaaaga agagacttct cttgattaaa
     4321 aaatagctat gcacacttgt gactttagtc atgagttagt agctaaaata gtcagcatat
     4381 agggaaactt atatgtagag ataggataga acctatccaa tactacttga attgctggaa
     4441 acccctaaag ctaaccaaac taaaacataa ggatgaaata aacctaagtg tgaaagttac
     4501 gaaagtagaa aaaattggtt agatggtata aggttaaatc ctaagtactt taaaatgggc
     4561 aatcagcagg taagctccga ataggaaaaa cttcaacgac tattcctctt gagggaagta
     4621 cactacaagc tatcggtagt ggaagtgggt agaccttaac ggataatgcc gaaggataag
     4681 atatagtctg tgcttgcatg aaagtgtaag aagttcataa gagaactgca taggtagtag
     4741 cgaacttatg tgaacgacaa cctcaaaaat gataaaagtt aagatttgcg tttcttacgt
     4801 atgttattta aaaataaata taaaaacctt aagaatactt gttttaagta attactatgt
     4861 taatattatg ttgggtgatg aaaaatggaa gtgactcatg gtagagggta tgtgtattta
     4921 gtacaatacc atatagtatg gtgtgtaaaa tatagacatc cagtattaac aaatgaaatt
     4981 gaagaagatt taattaatat attaaacaaa atagctgaag ataataaatt tcaaatatta
     5041 gaatgtaata gtgataaaga tcacatacat ttactaataa attgttcacc acaacactat
     5101 ataccagata taataaaagc attaaaaggt gtatcagcaa ggctgttaat gaaaaaacat
     5161 ggagaacagt tgaaaaagaa attatgggga ggacatcttt ggaatcctag ttatttcgtt
     5221 gctactgtat cagaaaatac agaagaacag ataagaaaat atattaataa tcagaaaaaa
     5281 caagtaaagt gaggtggcag tagtggaaaa agcttataag tttagaatgt atccaaataa
     5341 aaaacaacaa gagttaatta acaaaacttt tggatgttgt agatttgtat ataataagta
     5401 tcttgctaaa agaatagaag tttataaaaa caataaagaa acttttactt ataaacagtg
     5461 tagttctgat ttaaccaatt taaaaaaaga attaaaatgg cttaaggaac ctgacaaatt
     5521 ttcactacaa aacgccttaa aagatttaga taatgcatat aaaaaattct ttaaagaaaa
     5581 agcaggattt cctaaattta aatcaaagaa aattaataga ttttcatata aaactaactt
     5641 tacaaatgga aacattatgt attgtggtca atatataaaa ctacctaaac ttggtatggt
     5701 taaggtaagg gataaacaag tacctcaagg tagaatactt aatgctacta tatctaaaga
     5761 accaagtggt agatattatg tatcattatg ctgtactaat gtagatattg aagtatttga
     5821 aaatactaat aatcagatag gtttagattt aggaattaaa gaattttgta tctcaagttg
     5881 tggagacttt atagaaaatc ctaagtatct taagaagtca ttaaacaaac ttgctaaatt
     5941 acaaagagaa ttatcgagaa agacaatcgg tagtttaaat agaaataaag caagattaaa
     6001 agttgcaaga cttcaagaac atatagctaa tcaaagaaat gattttctac aaaaactatc
     6061 tactaaatta ataaaagaga atgatattat ttgcatagaa gatttacaag taaagaatat
     6121 gattagaaat cgtaaactat ctcgcttaat ttctgatgtg tcatggtcag aatttgttcg
     6181 tcaattaaaa tacaaggcta attggcatgg tagacaaatt gtaaaggtag gtaaattctt
     6241 tgcaagttca caaatatgca ataaatgtgg gtataaaaac gaggaaatg
//
LOCUS       DAGSHT010000190         6215 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGSHT010000190 DAGSHT010000000
VERSION     DAGSHT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.196"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            168..1493
                     /gene="cdeA"
                     /locus_tag="MJB72_003084"
     CDS             168..1493
                     /gene="cdeA"
                     /locus_tag="MJB72_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBY8264157.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1588..2382)
                     /locus_tag="MJB72_003085"
     CDS             complement(1588..2382)
                     /locus_tag="MJB72_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264158.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2383..3258)
                     /locus_tag="MJB72_003086"
     CDS             complement(2383..3258)
                     /locus_tag="MJB72_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264159.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3262..3630)
                     /locus_tag="MJB72_003087"
     CDS             complement(3262..3630)
                     /locus_tag="MJB72_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8264160.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3813..4472)
                     /gene="deoC"
                     /locus_tag="MJB72_003088"
     CDS             complement(3813..4472)
                     /gene="deoC"
                     /locus_tag="MJB72_003088"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBY8264161.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4508..5695)
                     /gene="murJ"
                     /locus_tag="MJB72_003089"
     CDS             complement(4508..5695)
                     /gene="murJ"
                     /locus_tag="MJB72_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBY8264162.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5772..>6215)
                     /locus_tag="MJB72_003090"
     CDS             complement(5772..>6215)
                     /locus_tag="MJB72_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8264163.1"
                     /translation="GSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIE
                     DLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGY
                     KNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2051 a          976 c          686 g         2502 t
ORIGIN      
        1 agttagattt tactaatata agtttattaa ttagcattat actaatttat cttaatatga
       61 ttttttgttg attttaagct gtttatcgta taaataaaat ttttcttgac tatatagtta
      121 ctatatagct tattatatat agagagtaat aaaaggaggt aaaatttatg gaaaatttat
      181 ttacaagaaa attcactact tttgaatttc taaaatttgt ttctcctgca attatatcca
      241 tgatatttat atctttgtac acaataatag atggcatctt tgtatcgaca ttagttggtt
      301 ctgatgctct tgctagtata aatattgtac tacctataat taaccttgtt tgtggatttg
      361 gaataatgat ggcaactggt ggaggtgcta tcgtttctat acgtatgggt gaaaatagac
      421 aggatgaagc caactctaca ttttctttta tagttttgtt ttcattgatt gttgggattt
      481 tattcacagt aatctcatat ttctttatca aagaaatatc tattttgctt ggtgcaacag
      541 ataagttatt accatattgt ataacttatg gtaaagttat gattttatgt acaccatttt
      601 atattttaaa atttatattt gagtactttg caagaactga tggaaattct aaatttagtt
      661 tatttctatc agtcattggt ggtgtaacaa atataatatt ggattatgta tttattaaat
      721 attttggaat gggtctttta ggagctgcag ttgcaactgc tataggtatt attttaactt
      781 gtgttttagg tattatttac ttcttatcta ataaatctac actaaaatta agaaaaccaa
      841 aaaccgattt tagacttata agagatacta tgataaatgg ttcttctgaa atggttacag
      901 aattatctac aggaattaca acattcttat ttaatgtagt agctttaaaa ttagcaggag
      961 aaaatggact tgctgctctt actatagtat tgtatgctca ttttttaatg acatcagtct
     1021 atctaggatt cgctgctgga gtgtctccat taataagcta taattttggt gctgaaaaca
     1081 gtgataaatt aaaagaaaca tttaaacatt ctctaaaatt tatatttgtt tcttctcttt
     1141 tagtgtttat ttttgcttta gtatttgcac catttattgt tagggtcttt gtaagtccag
     1201 ataacacagt atttaaacta gccttacaag gattaaaaat atttgcattt gcttttttgt
     1261 ttgttggtat aaatatattt gcatcaggat tttttacagc atttcacaat ggaaaaattt
     1321 cagctattat atcttttagt cgtgcctttg tttttataat cataggaatc ataattcttc
     1381 ctcctatgtt aaacatgact ggattatggc ttacagttcc atttgctgag gttataacca
     1441 tatttatatc tattctattt ataaaaaaat ataaatgtag atataagtat taaaaattaa
     1501 tacttaaaat caacatttat taaaactaat aaacctgctt taaaatacaa atttacaaat
     1561 ttgattttta agcaggtttt attttagtta taataagtca tttgcatact ctttaattgg
     1621 tgcttttcta agtaataaaa ttataaatat tatgcaaata actataattc ctacttttgt
     1681 tccaaataaa ataaaaggat tctgaaatga ataaataatt ttttctaaat acaatagcat
     1741 tttatgcata tttttagttg caaaattaac tatataaatt atcattaata tcggtaatcc
     1801 tatcaatata gtaatactag taacttttct aactctatat gtaaaagctc caagtaacac
     1861 tcctataaaa cctatacata tttcatttat acatatttgt acaaaataat ttaaataagt
     1921 tagtggcata cttgatgttg aaggatctat cagtgctgat atagatgtcc aaatcatatc
     1981 tctctttagt aaaactgata gttctatact gtaacctgtt ataatttcta taaataactt
     2041 cgataaaaaa atcaaaacta tacctaaaat agaaatcaca aagcttaaaa taattaaatc
     2101 tatgacaata gcttttatac aactttttct atctgctctt atacttaaag ctcctgaaaa
     2161 gtctttattt gctatattta ctccatacac aaatagcagt atagttatat atgttaaaat
     2221 aggattcaca atcgtggata aattagtttt agatgcttct ccaaaaatct tgagaataaa
     2281 cactggcaaa atattcataa caattcccac aatcataaat ataattatat acaatttagt
     2341 atttttatta aaaaatttta tatatggctt taattcattc aattacaatt cctccttctt
     2401 agtcatgtta acaaacatat cttgtagccc tatgtatcct acatcaatat ttgaattttt
     2461 aagtattttt tcatcacttt cactaaaatc accatagtaa aaataagcaa ctgcatttcc
     2521 aaatgccttt ttaggtttta tattttttat acattctaat ttttctaact cttctttatt
     2581 tccagtaata taatgagctt tttgttttat gacatcaatt tcttcatcta taataatttt
     2641 tccctcattt aatattatta catgctccag taaatcatct atttcatcaa tcaaatgagt
     2701 tgatattatt attgtccttg gattttttat ataactatct aaaattatgt tataaaactc
     2761 ttgtctattt actgcatcca gaccaattgt tggctcatca aatatggtga tagctgaatt
     2821 agaacaaata cctattatat ttgaaagtat agttttcata cctcttgaca gttgtttata
     2881 ctttttttta gtatttaaat caaaatattt gcataattct tcttctaagc ctttatcata
     2941 acttttataa aaactagaag aaaattcaaa tatctgacct actttaaaat ctggactata
     3001 aaattctttt tccctaacga tacaaacttc ttccaaaact tttatatctt cttttgggtt
     3061 ctttcctaat atttctatct caccttcatt ttgaattaac tgatttacca ttatatttaa
     3121 taatgttgtt tttccagctc cattcttacc taataaacca tatatcttat ttttttcaaa
     3181 gcttagagac atattattaa aaatattttg cttcttaaaa ctgtgagata aattttctat
     3241 cttaatagaa ctactcatat attaatcctc cttgaaatta attataatat caaccaattc
     3301 ttctctattt atttctaatt tgttagcttc ttgaagtagg ttttttacaa aattatgttt
     3361 aaaattttct tttctagctt cttttataat agtttttgca ccatctgata caaacatccc
     3421 tatccccctc ttcttatata aaatattttt atcaaccaat atatttatac cttttaatac
     3481 tgtagctgga ttaattttat aaagctttga aagctctgtt gttgatggaa cctgttcttc
     3541 ttcttttatt ccatttgata gtatctcatc ttcaattgct cgtgctatct gaataaatat
     3601 aggttcttca ttatttaata ctaatttcat aggttcctcc tttctccatc agttagttac
     3661 tcaactaatt aactatataa cttataatat cttgaaaaat attttctgtc aatactaaat
     3721 aaaaaacaga gggaaaattc cctctgtttt ttgttcttta tatttataat taaattttga
     3781 tttttgtaat cctatcccat aagttaaaaa ctttaataat ctgttgtatc ttcatttttt
     3841 aatattttac atattgagct agttcctaac ctttctgctc ctgcctctat aaatttttct
     3901 gcatcagaaa ttgatttaac tccccctgca gcttttattt ttacattttt tccaacatgc
     3961 tctttcataa gttttatatc ttctaaagta gctccacctg ttcccattcc agtagatgtt
     4021 tttataaaat ctgaacctga cattgttact atttcacaca tttttatttt ttcatcttca
     4081 tctaaataac atgtctctat aatgactttt aatactttat ctcctatagc tttttttatt
     4141 tcttttattt cttttcctat attatcataa tctttatttt ttatatctga aatgtttaca
     4201 accatatcaa cttcatctgc tccattttca atagcatctt ttgcttcaaa tacttttgta
     4261 gcagtagttt gatatcccaa agggaaccct atcactgtac atatctttat tttacctttt
     4321 agatattctg atgctctttt cacatatgat ggtggtatgc aaactgatgc tacactcata
     4381 tctacagctt catcacataa aattttaata tcttcccatg tagttgtagc ttttaaaata
     4441 gtatggtcta ctgtttttaa tatatgtttc ataaaatcct cctaaattta taattaatat
     4501 aattagatta cttttttaaa tgcaattttc tctttgcaat gtttagtatc atatcaacct
     4561 cttcaacttt aaaaataatc attagtagtg tataaattcc acctccaact ataactgaaa
     4621 tagctagtga tacaaactca ttaaatgttc ctactcccaa aattccaaat ataaatttat
     4681 acgtaaagta tgataaaaca cccataatta aagatgcaac taccgatttc aaagttgctt
     4741 ttattatttt atcttgtcca aaatatccaa cttttctctt taggttcaag aagagtaaca
     4801 atatacatgc aattgatgat gaactagttg caagagcaag accaccatga gccattggtt
     4861 ttattaaaac taagtccaat actatattta cccctacaga tataatccca tttaccatag
     4921 gtgtttttgt gtcttgtaat gaataaaaaa cctttcccaa aatatctctt aagccaaatg
     4981 cagtcatacc aactgcataa aaaattaagg cagttgctgt catttgggtt gccctcgcat
     5041 caaaagcacc tctctcaaaa atgattctaa caacaggagt tgcaaaaaaa atagaagcaa
     5101 ctgaaatagg aatcatagaa attattatca tatttatact ggatttaact gaactagtaa
     5161 acttcttttg attattttct gcacttaact tagataacat aggatacata acagatacta
     5221 ttgaagctgt aaatgtccct gtaacaaaac catttaatct atctgcataa gtaagtgctg
     5281 gaatgcttcc tttaactaat gttgaggcta aagttgtatc taccaatgag ttcacttgat
     5341 taacagcaac acctataaat actggtgaaa gtaatgctag taatttaata aggctatcat
     5401 ctttaaagtt taagtagtat aagtatttgt agttcgtttt tttaacaaaa aacatgtaaa
     5461 agagcagttg aactaccata gcaaccacag caccaattgg aagtatgtat ggtccaaata
     5521 tggtacttaa cattattgaa ataattatta ctatattata gggtatactt ccaaatccaa
     5581 ctacaataaa gttctcttta atttgtaaaa atgctgacat aacactcgta atacctatga
     5641 atattattcc tgttctgaac ctcccatgac taaagtcaca gggttcctgc ttcatagaat
     5701 tttgcatact aaaatatgtc ttactaattc tccacaggct atccccgtag ttcctacggt
     5761 tcttacatac gttatttatt ttgaattgtt attagtctta atccttcttt taatatattt
     5821 atacttgcat ttatatctct atcgtgagtt tcattacaac taggacaaat ccattctctt
     5881 atatttaagt tcttaacttc ctcgttttta tacccacatt tattgcatat ttgtgaactt
     5941 gcaaagaatt tacctacctt tacaatttgt ctaccatacc aattagcctt gtattctaat
     6001 tgacgaataa actctgacca tgatacatca gaaattaagc gagatagttt acgatttcta
     6061 atcatattct ttacttgtaa atcttctatg caaataatat cgttctcttt tattaattta
     6121 gtagatagtt tttgtagaaa atcatttctt tgattagcta tatgttcttg aagtcttgca
     6181 acttttaatc ttgctttatt tctatttaaa ctacc
//
LOCUS       DAGSHT010000191         6176 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGSHT010000191 DAGSHT010000000
VERSION     DAGSHT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.197"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..468)
                     /locus_tag="MJB72_003091"
     CDS             complement(<1..468)
                     /locus_tag="MJB72_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8264164.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKP"
     gene            complement(838..1740)
                     /locus_tag="MJB72_003092"
     CDS             complement(838..1740)
                     /locus_tag="MJB72_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8264165.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(1790..2251)
                     /locus_tag="MJB72_003093"
     CDS             complement(1790..2251)
                     /locus_tag="MJB72_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBY8264166.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKAYELWSGNIMF"
     gene            complement(2303..2758)
                     /gene="def"
                     /locus_tag="MJB72_003094"
     CDS             complement(2303..2758)
                     /gene="def"
                     /locus_tag="MJB72_003094"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBY8264167.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(2730..2993)
                     /locus_tag="MJB72_003095"
     CDS             complement(2730..2993)
                     /locus_tag="MJB72_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264168.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(3090..3806)
                     /locus_tag="MJB72_003096"
     CDS             complement(3090..3806)
                     /locus_tag="MJB72_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264169.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTSLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(3803..4486)
                     /locus_tag="MJB72_003097"
     CDS             complement(3803..4486)
                     /locus_tag="MJB72_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264170.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANFYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(4483..5328)
                     /locus_tag="MJB72_003098"
     CDS             complement(4483..5328)
                     /locus_tag="MJB72_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264171.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(5442..6032)
                     /locus_tag="MJB72_003099"
     CDS             complement(5442..6032)
                     /locus_tag="MJB72_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8264172.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
BASE COUNT         2184 a          983 c          586 g         2423 t
ORIGIN      
        1 aggttttaaa ctatcataaa ttttttccat taataacctt ctattttcag ttgacaaagc
       61 tccataatca caataaatta aagtagccaa atcaaattcc tcattatagt tcatatttaa
      121 ataactttga aacaagtaat ttatgtttaa attagtctcc ttatttctat tttgtgcata
      181 gttaatagaa cgttttgaga aatcaattcc agttacctta taaccttttt gtgcaaattt
      241 ctctgcatat aaacctggac cacaacctaa atcaagtaat ttaggataat tagctggatt
      301 tgcaactgta actatccaat ttacagaatc ctcgataaag ttaaaatttc tacttgctcc
      361 ttcaaaatta gtgtctaagt gtgcttttaa taattgcttt gaaatatact catcatccca
      421 aaaattaatt tcactttgtt tataaagctc tggtctttct aaatacatac ataatttgtt
      481 taacaaaaca taccactcct taaaatttat ttttaaaata aaaaaaccac agagtttact
      541 ctgtggttta acttcaaaaa gatgagttta aataagagta aaatagaacc aaatattttt
      601 aagcacaaca ataaaactac agctttactt tgtttgctta ttaaatttga ttttattcac
      661 tcttttccag caatacttat atcaacgatt ttatgatatc aaatatcacc gaacaacatc
      721 atcctttcat atttttattt tatgaattaa ttatatattt aaattttata tttgtcaata
      781 aattttaatt ttaataaatt ttaatcatca tagtaaaaaa aattttttta tataaaacta
      841 atcaaaaaaa gatatctgta catctcctga gttttttaac ttttccttca ttattttaga
      901 gtaactgctt gatagagaat acttatatct aagttcatta accattttat acaattgatt
      961 tttatactcc ctattaagtg aaccttcact atatagtatt gataatttat caaataattc
     1021 aggaaactct tttttaacaa agtcaaaaaa aacacctcta gttatacccc ttaagtataa
     1081 agtcccaggt aaaacataat gtacattaca ttcttttgca tgtctaaata gcgaatttat
     1141 attatcaaaa tcatctgtaa tatatggtat aattggcata atatgtagac caactgatgc
     1201 gttagttttt ctaaattctt ttaacatttt aaatcgtctc attgaatcca caccatttgg
     1261 ctcaataagc ctttgtgtat cttcatttac tgtggtaatt gtagatgcta cattgatata
     1321 tgtaatccta gatagcttat caattaaatc gtaatctctt aaaattaaat ctgactttgt
     1381 agaaataata gctggtgtct tgtattttat tagcaggttt aatatttctg gcataatttt
     1441 atagtcagct tctattggtt gataactatc tgtaactcct cctatattga ttacttcttt
     1501 cttccattta ctgcttttaa gttgtctctc aagtttttct actatatttg tttttacata
     1561 aatctcttca aaataattat tggagttaat gtacttatga gagtaaatag cataacaata
     1621 tttacatcca tgctcacatc cacgataaat atttaaatcc catttatatg gcatactcct
     1681 ttttaattta ttacaagcta tttcacaatt tattggaata tattttattt tattatacaa
     1741 ataatcacct ctttatatac aataatacta taatgttaat ctatagtaat taaaacatga
     1801 tatttccaga ccacaattca taagctttac tatatttaca aagtaatata ttccattcat
     1861 ttattggtat tcctccacat gttgcttttt cttctaaatt atataagcat tgctttaaat
     1921 acattttatt atgccaatca tcatataaat tttctaaatt taaactatgt tcattagata
     1981 tatcatttgc aaaatttcta tttttccatt ttaatatact tgtatattta agttcattag
     2041 gtttataatt tctttttaac atttcatcat gtacaatttt tgtataaaat ttaaattcat
     2101 ctatactata attcaatact ttattcacta atagatggtt tggagtacct tttttatcta
     2161 tagaaccttt tatagcaagt agctctctcc attgacttac taattgattt ttaggtaata
     2221 catcaattaa atccttatgc cataatctca taatttaact ccttactata aaatatttta
     2281 taaattcaat aaccaatcta tcttacaatt ttacatgttc aataatttta tctgtaaaaa
     2341 ctataccatt taaatggtct atctcatgac aaagacattt tgccataaaa cctgaacctt
     2401 taatttctat tttctcaccg tattcgttta gagcttgcac tgtaactttt tttggtcttt
     2461 ttaatttacc ccatacttca ggaaaactta aacatccttc aacaacaatt tgttcccctt
     2521 cttgtttaat aatttcagga ttaacaagct taataagtcc ttctcctaaa tctataacaa
     2581 ctaatcgctt taatactcct acttggcatg cagctaatcc tcctccattt ggagtattgt
     2641 acatggtctc tgccatgtca tttaatattt ctcttatctt attatctacg ttttctacat
     2701 acttactctt ttttcttaaa atttcatcat caaatgttct tatctttctt atagccataa
     2761 tattctcccc tacttatctg tgctcttgat tttctttcac tttcttcaaa taaaaataca
     2821 ctttcaattg gttcattaaa aattcttgaa atatcatatg ccaaccatat tgaaggttca
     2881 tttttaccag tttctatagc atttatagcc tgtctagatg ttccaactaa tctccctagt
     2941 tgttcttgtg tcaatcctaa tttttctctt aatatcttta ttttattttt catatttgct
     3001 ccattcatgt aaggttaact ttacatctta aatgtaacat ttttcaacat tactgtcaag
     3061 tcaaccttac atttatatat taaattttct tatttaacta tttttttact aaacttttta
     3121 aataataata aaatccatat taatgatata acaattgaaa taaacatata gacataatta
     3181 tcttccttaa atgccttcga tatccaaaaa gttggtaaga agaacagaat atattgtgct
     3241 ttatttaaaa taaaaaatgg tgctaaaata cccatcataa aaacacctga gaatttcgta
     3301 attaccattc cttctagttt atttgatgat aaagaaatca ctaatattga tataataatt
     3361 ccttgtaata aaactaataa agaaattgat aagtttaaca agaaagaaat tttagttaga
     3421 gaaaaaaaga taagtagaat catagtaata ataaatgcta agatacctgg tattccaaat
     3481 cgtgaaataa gataaccagt tttaccaagc gaagttacta ttaaatatct tgaaatgctg
     3541 tcatcaattt ctcccaatat gaccattgta gaagcaaaaa agaacataaa aggtgtaata
     3601 aaagctaaaa gtaaatcaaa catcaagaaa tacggctcta aatttaattg ataataaaac
     3661 ttgttaagtg atatattctg tattaatggt attccaaatt ttataaatac tccacataat
     3721 attggagaaa cacatactat aaaaagcata gcatctctct taatttgttc aaactcttgt
     3781 ttaaacgcat ttataatctt tatcatagtt ttactccacc tacctttttc caactgctac
     3841 aaataaaata ataagttata aaataaatta ttattataat tgatattaaa attaaaatat
     3901 taatcattat aaaattttta tctccactaa taagagaaat acatatatta aaagggtaaa
     3961 aatttgcata tgattttgtg tcaagaagta cattgagtat tggtggaata aagcatataa
     4021 tctcaatagg aatacttaaa acaataaact gattcaaact agatgcttta cttgcaagta
     4081 tcaatccaag caaagaaaat ataatagaag aaaaaaaggt acctatagtt gatattataa
     4141 tattatctaa atttaatgta atagcaataa acatagctac aatactagat attactccta
     4201 atgaaattac ctttgataaa atatactcag atacttttac tggagataca gctaaggaat
     4261 ttaaaaccct ttgacttttc tcaagaagta caatagctcc cataaaaaac aatcccattg
     4321 ctgctgggtc agaataaatc ataattatag ctattttttc tctcataaaa gttggaaata
     4381 cattgataat acaaatatat ataatactta aaaaaagata taaaaaataa aaaccatact
     4441 taaattgaaa ctttatatct cctaaaataa gattttttag cctcattgta aattcctccc
     4501 tgttatctca ataaaaatat catttaatgt tggttcacta ctatgtattg ataaaagttt
     4561 atttttctca ataagcatat tcaaattttt atcattagca gtattgttca gaaaacattc
     4621 agaagttttt tctccattat caaagtaagt atatctaact ttaatagctc cttttgacat
     4681 aataagatta tgtggtgtat ctaaagcaga tattttacca tttacaatga acgctactct
     4741 gtcacaaagc tctgttgcat ctaacatgtt atgagttgtt aaaataatag ttttaccttt
     4801 agatttttct gataaaatta tatctttcat aaccttgcta tttgacgggt caagaccact
     4861 tgttggttca tctaaaaata aaatatctgg attatgtaat aaagctttta taaaatttaa
     4921 tcgagatttc ataccttttg aatactcaga taccctttta tttgcttcat tttctagtcc
     4981 tactgatttt agtaactcat caatagatag tagtttttta gaatatagag acccaaaata
     5041 ttttaaattt tctattgcag tcaacttttc atataaacta ggaaattcaa aatcaactcc
     5101 tatattttca taaaatttat ttgagtgtct tttactctct actccattta caatgacact
     5161 tcctccataa ttagttatca ttccaattag aatcttttgt aatgtagatt ttcctgcacc
     5221 tgatggtcct aaaaatccta gtatttcccc ttttcctact tcaaagttaa tattttgtaa
     5281 aaaatctcta tctgtataac taaatgataa atcatcaact ttaatcattc aatatcaccc
     5341 tttcaaattt ttgactattt tgactatatg gtcaataaaa tataattttt ttgtttgtta
     5401 ataattttta ataaaagtta ttagcaaaca aaatttaaat tctattccat aagttgtata
     5461 actaatccgt ttatcattaa tcttaaagca tcttcaaaac aattacttcc aacctcgcgc
     5521 ttatatagca tggtaagaaa aactgctcta aatgctccac taaagctttc tatgtcttta
     5581 ttttttgcat ttggtatgca agatataatc tctccaattt caaagtcatc atgtctaaag
     5641 tgttcttcaa ttatagaatc tggtaacttt ctcaccaata tctcaaattc tccatttatc
     5701 atgagattta aaattgaagt actatcaacc atcctataga atttcataaa tatatctgtc
     5761 aattgttcac atgtaatact ttcatctact ctatttatct catataagat ttctttttgt
     5821 atagagtcat gtatatctct taatacctca aaaaagagca attcttttga ttgaaaaaat
     5881 agataaaaag ttccttttgg tatttttacc cgttttacaa gctcatcaac agtagttttt
     5941 ctaataccat attgctccat acacaatctt gcttcttctt taagtctttt tattatatac
     6001 tctctttctt tatcagaata tgatttaggc atcttttccc tccagtttga ctaaaataat
     6061 ttatctagtc aatataatac tttttctatc taatgtcaat agtattatta gtatatttaa
     6121 aaataagctt aaaaactttc gaaaattctg aattttatat agcatttttt attatc
//
LOCUS       DAGSHT010000192         6174 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGSHT010000192 DAGSHT010000000
VERSION     DAGSHT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.198"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            5..238
                     /locus_tag="MJB72_003100"
     CDS             5..238
                     /locus_tag="MJB72_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264173.1"
                     /translation="MINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQGDCEVLV
                     VFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            471..890
                     /locus_tag="MJB72_003101"
     CDS             471..890
                     /locus_tag="MJB72_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264174.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            915..5972
                     /locus_tag="MJB72_003102"
     CDS             915..5972
                     /locus_tag="MJB72_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBY8264175.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
BASE COUNT         2390 a          807 c          966 g         2011 t
ORIGIN      
        1 aaaaataatt aatatgatgg gaagttatta cacaaaagac tttgaaaaag aaaaaaagaa
       61 tgtaataaaa gttagagaag ttaaagaaga tacagttaga aaatttttct tacaaggtga
      121 ctgtgaagtt cttgtagtat ttgaagacac tgggaaagaa atacttattg atgacttttc
      181 tcctgaagaa gatatcaaaa aatatttagg agcaaaattt ataaataaaa aaagatagtt
      241 atcaaaaata attctaaaat taaaatttaa taaatgtaca gttataattc tatataatta
      301 tagttaaaag ttatctttta ttgctttttt agactttaag atagcttttt ttctattttt
      361 tgtaaaataa ttccataatt cacattaata attcacaaaa tgatataata taacaaatag
      421 ataaatttta aatttctaaa tacataatta atattttgcg aggtgatttt atgcaaattt
      481 attttagagt taaggctgtt ggcaaacgca aacctatgct agaacttacc ccttttgatg
      541 tatctgacaa catcaattgc ctaaaagaag ctatctctga aattgtatct aaaaatgttc
      601 ttgaatataa tgagaaaatc ccagaaaaat caataataaa cttcttaact aatgaagaaa
      661 ttgaatctca agctgatatt ggaaaagttg gatttggctc tatttacaat gagaataaac
      721 aatctgtaga aaaggcaata gaagtagcgt tacaggcatt tgaagatgga atctacaaag
      781 tgcttataaa tgaagatgtt attgaaaaac tcgattcctc tataaatctg aaaagtggag
      841 atatatttac ttttataaga cttaccttgc ttgctggaag aatgttttaa aataaataat
      901 ttggaggtac ataaatgtta gaacgtacta gagtagaaaa aaatcctaaa ataattgaaa
      961 attttaaaaa taattttact aaatcttctt caaaacagta ctacgactta gcaaagtctc
     1021 tttgggactg catagaaaat agttcatact attatagaga acctgaagac aaattcttaa
     1081 atatgataaa gaaatactac aattgccact actacgaaat taaaaatatg gacgagctcc
     1141 tttctggacc tttaaaaaat gcattagaat taatatttac ttctgaacaa gtagaagaac
     1201 tttgccaatt aattaaaaga aagatggatt atacatattc aggaggatat tatcgtaaag
     1261 ggtatcgctc aaataaactt gtggattatt ttagaggtat tataaggttt atttcaaact
     1321 atgttgattt aatttattat gatttttcag ttatagatta tcttactctt ccaaataagg
     1381 tcttgtacta tgactctata atgagtgata aaatagcact agaaatagac aaaggaaatc
     1441 aaaaagtaat aagtgcttta gaagaagtaa tatttggaga aaatcaaact gctctactta
     1501 caagacaaat gataattggt atcatgaaga gtcatagtga taaatgctat gaaatgcttt
     1561 ctaaacttct tttggcagcc aaacaacaag aaggacttag acagcaaatt gtagagagtt
     1621 tagatgaggg tacaatttct gcatcaatat acatgttaaa aactattttg gacaacaatt
     1681 tagagcgatt tagttctgtt ataagagcct tggatacatg gacaggactt ggtttttcag
     1741 accaaaaacc tggaataata cgtaaatgtt tagaactttc atataaatca ttgactgatg
     1801 aaaagttccg tatagaatca attaatagca atgataatct tgaaatttat tttgccttat
     1861 gggctactgc aacttacaac atatatgact cttactctct tattgaaaac ttactttctt
     1921 gtcaggaaaa atataaaaaa cttgtagctc tttacttttt atcgcataca gaaagtacat
     1981 attttaaaca ttctatagca tctaaaaaca ttaagggtga tgatttagag atactagcat
     2041 gggtaattac aaatttctat gtagaaggtt ctgttggcta ctactatagt tggcaaaaca
     2101 gagaaactaa ttttgacttg tcaaacactc ctattgacaa agactttgat gctagaaaag
     2161 aacaatttga aagtttaaaa catactttag aaattttaaa ttcaaagacg aaatcatttt
     2221 caggaagtgt atttccatgg acttggattg aaatttcttc tcatgaaata cttaaatgta
     2281 tgatgacttt aacagcatac gatatgaatg ctcaatttat agatgtgctt atggaaaatt
     2341 gtcatcttat gagttcagat ttaagagctt cacttatagt caattttgta aatgaaccaa
     2401 agacagatag acaacgtgaa tttttattgg aatgtttaag tgataagagt gtttccaata
     2461 gagaaacggt attgagtcta cttagaaagc ttgagctaac agatgtagaa ttagataaga
     2521 taaccaatct attgtcttta aagacaagct ctattagaca aacagtaata aaaattttac
     2581 ttgaacaaga tgattcaaac ttagaaaata ttgcttcttc actgataaaa tcttcaaatt
     2641 ctcaaaaatg ccttggaggt cttgaaattg taaacttatt aaaagacaat actgctagac
     2701 aagaactata taataagctt gttttactta tagaagatat tgataaatct aaaaagaata
     2761 catctcaagt tcaaatacta attgataatt tgttaggtaa agcaaaaaat gattactgca
     2821 aagaaaatgg ttttggaata tataaccctg aaaacaaagt tcaaattcca gaacaatttg
     2881 attttaaaga tgattctaat agctcatcaa agcttaataa aattatctca aatataagtt
     2941 tatctactcg tgaggattct gtttctgtag caacaaaaaa agaagcaaaa aaaattctcg
     3001 atacggatat aaataaaata ataaaagttt atgaaaaatt tgatgagctt attactgaaa
     3061 atgcagatta tgaatatgaa gttgaaagat ggtcaggtaa tgtagacaaa gtacttttag
     3121 gttattctac ttataacttg actcctatta aatataaagt ttctaatgat actcttgata
     3181 attatccatt agcagagctt tggagagaaa ctagtaaaaa acttgactta gattacaaat
     3241 taatacttga tatcttattt tatagtataa gtacatgtgg atataatgaa acagaaaaaa
     3301 gttggttttc atctatatat tcaaatgtcc ttgactctaa aaaacaaaat aaaatatata
     3361 caaaacttga aaaacttaaa tttaatggac ttacaagaaa acttttaaga gttttactaa
     3421 atgagtttga ccataaagaa atatttaact attgtttaga aatatactat aaacttagaa
     3481 acttattgac tatagaacaa tgtaaaaaac aaagagttga atttactaaa aactattaca
     3541 gacaaaatga atactgccct gctttaaatg cccctatttt aaactattgg cttgataaag
     3601 cacattcgtt tatttatgat gatatttctt ttactaagtt tttctacact acgttttcaa
     3661 attatcagtt gctagaatat aagtttcctt tctatactag cataaatgtg tttgctcgtg
     3721 cacatgaatt aggattaatg actgaagagc aagttaatag agaaatgata agtggataca
     3781 acgcatcaaa tataataaga acattaacaa atcctagatg ggctggcgac acattagaaa
     3841 agtacccttg gcttaaagat aatttaaaaa ctgttacaaa ccgaattata gaaattgaat
     3901 cacgtagagg tgatatgcca acagaagtgt ctaatctagc taataatata caatactttg
     3961 aaggtataaa ttattttgta aatattttgg tcgctcttgg taaagaaact ttccagagag
     4021 gatattcata tggtagcaat tattcaaaaa aagatgtttt aagttctcta cttaaaaatt
     4081 gctatcctac taaagaagat gattcaaaaa aattaaaaag tttacttaaa aataccgaca
     4141 ttactgagtt aagattgata gaatctgcta tgtatgcccc tcaatgggta aacataattg
     4201 aaaaatattt aaactggaag ggtctgaaaa gtacaatttg gtacttccat gcacatataa
     4261 atgaaggctt ctctgcacaa aaagaatcag aggttgctct attctcccct atttcaccac
     4321 gtagtttcat tgatggtgca tttgatgtag attggttttt agattcatat aaaactattg
     4381 gggaagaaag attcaatata ctttacaagt cagctaaata tataactagt ggtggtaact
     4441 ctcatagacg tgcacaactc tatgctgatg ctacacttgg taagcttgat aaagacatgt
     4501 tgatgaaaga aattgaaaca aatagaaatc aagataaatt gagaagtttc tctttactcc
     4561 ctatgaaaaa aggagaccta aaagaagcaa ctttccgtta tgaatttatt caaaagtttg
     4621 ctaaagaaag caagcaattt ggtgcacaac gtcgtgaaag tgaatcaaaa gcttgtacta
     4681 tagcgctaga aaatctagct agaagtgctg gatttggaga tgtaaatcgt atgatgtggg
     4741 ctcttgagac tgaaaaacta aatgaagttg ctcatctatt taaaccaaaa gatattgatg
     4801 gtacttcaat ttggatagat gttgatgaaa acggaaaagc atctactaaa gttcaaaaag
     4861 gcaacaagtc tttaaagagt ataccaaaag ctattgctaa acatccatat gtacttgaac
     4921 ttaaatctgt ccaaaaagat ttaaagagtc agtattctcg tgcaaaacag agtttagaac
     4981 aagctatgaa aaatgaaata gtatttacag ttaaagaaat tgccaatatc tataaaaatc
     5041 cagttatctg ccctttgatt aaaaatctag tatgggtatg tgatgataat attggatatg
     5101 tttataatga taagtctaag ttctttattt gcaatataga aggtgaaact tataagttga
     5161 cggcaaaaaa taaattaaaa cttgcacatc cagttcattt gtttgaatct ggtaagtgga
     5221 gtgagttcca acatgattta tttagtaagc aaattgttca accatttaag caagtattcc
     5281 gtgagtatta tagaataaac agtgatgaat tatctgaaaa aactatatct cgcagatatg
     5341 ctggtcacca aattcagcca aagaaaactg tagcacttct tagaaatcag ctttggactg
     5401 tcgattatga agagggatta cagaaagtat tttataaaga aaatatagtg gcaaagatgt
     5461 ttgcacttgc agattggttt tcaccagcag atatagagcc tcctactatt gaaacagtag
     5521 aattttttga tagaaaaact tataaaagtt tagaattagc ttctatacct ccaattctat
     5581 tctcagaagt tatgcgtgat atagaccttg ttgtaagtgt cgctcatgct ggtggtgttg
     5641 accctgaaac tagccattct actgtagaga tgcgtatagc aattgctaaa gaacttttat
     5701 tattattaaa aattaacaat gtcaattttg aaggctctca tgctcttata gcaggtaatt
     5761 taggtgagta ttctgtgcat atgggttctg gtattgtaca taaaaaaggg atgggtgctt
     5821 tgaatatact tcctatacat tctcaaagta gaggtaagat attccttcca ttcgctgatg
     5881 aagaccctaa aactgctgag attatgtcta agatactatt acttgctgaa gatagtaaga
     5941 ttaaagaccc tagtatctta agtaatttat aagtataaaa actaccccta aaatatattg
     6001 gactttctta aaaaaattag aaagtccaat atataatata ctgtaattta tatagattaa
     6061 atcataatta caaataattt ttatataaac ttctcttata cattatcttt atggtactta
     6121 ttatgtcaca caaaaagtat tttccttcta ttttcactag aatcttttaa ccct
//
LOCUS       DAGSHT010000193         6118 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGSHT010000193 DAGSHT010000000
VERSION     DAGSHT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.199"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..98
                     /locus_tag="MJB72_003103"
     CDS             <1..98
                     /locus_tag="MJB72_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8264176.1"
                     /translation="NDKSVSMDTMEKICLALDCKIEDVVEIKKGI"
     gene            262..1416
                     /locus_tag="MJB72_003104"
     CDS             262..1416
                     /locus_tag="MJB72_003104"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBY8264177.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1429..2433
                     /gene="rseP"
                     /locus_tag="MJB72_003105"
     CDS             1429..2433
                     /gene="rseP"
                     /locus_tag="MJB72_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBY8264178.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2615..3670
                     /gene="ispG"
                     /locus_tag="MJB72_003106"
                     /gene_synonym="gcpE"
     CDS             2615..3670
                     /gene="ispG"
                     /locus_tag="MJB72_003106"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBY8264179.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4018..4842
                     /locus_tag="MJB72_003107"
     CDS             4018..4842
                     /locus_tag="MJB72_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264180.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5316..>6118)
                     /locus_tag="MJB72_003108"
     CDS             complement(5316..>6118)
                     /locus_tag="MJB72_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBY8264181.1"
                     /translation="FVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLY
                     KFLVIKANETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKY
                     IISSNLMCYTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIA
                     YLTSIKHPNIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFW
                     QKYEGKINEISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
BASE COUNT         2352 a          642 c         1111 g         2013 t
ORIGIN      
        1 gaaatgataa atctgtttct atggatacaa tggagaagat atgtttagca ctagactgta
       61 aaatagaaga tgttgtggaa ataaaaaagg gcatttaata atactagaat attttataat
      121 attccacaaa atacaaaaaa taatagatta attttaatct gtggaataat atatgaaatt
      181 tctatgaata gtaaaaataa ttgttttagt agtgttataa tgttttgaca gggtatattt
      241 attattagaa aggattatta tatgaaaaag atatcaattt taggttctac aggctctata
      301 ggtaaacaaa ctttggatgt agttagagat aatagagata agtttgagat agttgcaata
      361 tcagctaaca gtaatattga attattgcta gaacagatag tagagtttaa acctaaatat
      421 gtaacagttt ttgaggaaaa taaagcattg aaattaaaag aaatgttgcc taaaaatata
      481 gaaatagaag ttttagcagg aatggaagga ttaaaaataa tatcatcact tgatgaagtg
      541 gatgtccttt taactgctgt agtaggtatg ataggattag tcccaactct ttgtgctata
      601 aagaagggaa tagacatagc attagcgaat aaagaaacgt tggtaactgc tggtgaactt
      661 gtaatgaaag aagcagaaaa atacaatgta aatattcttc ctgttgatag tgaacatagt
      721 gctatatttc aatgtctaaa tggagaaaat aaaaaaaata tagaaaagat aatacttaca
      781 gcatctggtg gtccatttag aggaaagaaa aagggagaac ttgcaaatat aactaaaaat
      841 gaagctttga agcatccaaa ttggagtatg ggaagaaaaa taagtattga ttcttcaacg
      901 cttatgaata aaggacttga agtgatagaa gctagatggt tatttggagt agaacaagaa
      961 aatatagatg tagtagttca cccacaaagc ataattcatt caatggtgca gtatacagat
     1021 agctcaataa ttgctcaatt aggatgtcca gatatgagac taccaataca gtatgctctt
     1081 acatatccag atagaatgga aagtagcttt gaaagaatga atttttcaaa atttagtact
     1141 ttaacttttg aagaaccaga tttagaaact tttccatgtt taaaattggc atatgagtgt
     1201 ttaaaaatgg gaggaactta ttcttctgtt ttaaattcag caaatgaagt tttagtaagt
     1261 gaatttttag aggataaaat tggtttctat gacataccat attacataga gaagacttta
     1321 gaagttcata gtagcataag tgaaccaaca ttagaagaaa tcttagaaac agatagatgg
     1381 agtagagctt atgttgcaaa tctgataaaa aaataggaag gtgaatttat gactataata
     1441 gccgcattaa tactatttag tataattgta ttaatacatg agttaggaca ttttatattt
     1501 gcaaaaagaa gtggtatcaa agtaaatgag ttttctatag gtatggggcc taagatatat
     1561 agtgtaaaaa aagatacaga gtattctata agagcacttc caattggtgg atatgtcagc
     1621 atggaaggtg aagatgaaga acaaataagt ccaaattctt ttggaaacaa gtctatatta
     1681 caaagattta gtactattgt agcaggacca atattcaata taatattagc agcaatactt
     1741 ttagttccag tatttttgta tataggttct ccaacgacta aattaggaaa gataatgcca
     1801 gatactcctg cacaggctgt agggcttcaa gtaggagata agataaataa aattaatggt
     1861 aattctgtta agacttggga tgaagttgca aatataataa atacttcttc tggtggagaa
     1921 ttaaaactta gtataactag agatggaagt gataaagttg taaatgtaac acctaaaaat
     1981 aacaatggaa agtatgaaat agggattcaa ccacaaagag aaaaagactt tttggggtca
     2041 atagtaaatg catgtaaaac tactgtagat atgacaaaac agatgctaac atttctaggt
     2101 caaatgatta caggacgtgt accaggaggg attggcaatg ctgtagcagg accagtaggt
     2161 gttattggta tggtatcaga tgctgctcgt acaggcatca taaatgtagt gtatttagca
     2221 gctgtcataa gcttaaattt aggtatagta aatctattac caataccagc acttgatgga
     2281 tggagaatac ttatgctgtt actagaagct gttagaggtg gtaaaaaact cgacccaaat
     2341 aaagaaggca tgataaatgt tgttggtttt ggagctttaa tgttatttat gctttttatc
     2401 acatataaag atatactaag attatttcaa tgatttagtt tttagagatt ttaagaaaat
     2461 aggctatgta gttttaaata agaataaatt aatttttata tatgaaattt attcttattt
     2521 aactagatag ctttttaatt tataatatac atgtaggtta taatatttta atatttaaag
     2581 agattcaata taaaataata tatagaggtg gaatatgagt tacgagagaa gaaagacgag
     2641 agaagtaagt gtaggaactg taaaaatagg aggagaaaat cctataagta tacaatctat
     2701 gacaaatact gatacaagag atgcagatgc tacaatagct caaataaaga gattagagga
     2761 agctggttgt gatatagtta gagttgctgt tcctgatatt aaagctgcta aaaatatagc
     2821 aaagataaag tctagtgtaa atataccaat tattgcagat atacattttg attataagct
     2881 agcccttgaa gctatagaac aaggtgtgga tggaataaga ataaatcctg gtaatattgg
     2941 aagtatagag agagttaaga tggtagttga gaaatgcaag gaaagaaatt taaaaattag
     3001 aattggagtt aatggaggtt ctttagagaa agaactttta aaaaagtatg gctctgcaac
     3061 tgctgaagca ttagttgaaa gtgcaatggg acatattaag attcttgaag acttagattt
     3121 tcataatata gttatatctc taaaatcatc cgatatttat aaaactgttg atgcatatga
     3181 attaatatca aaaaaagttg attatcctct tcatattgga attacagaat ctggaagtgt
     3241 tcacaaagga acaataaagt cttctatagg ggtaggagca cttcttctta aaggaattgg
     3301 agatactgtt agaatatctt taacaggcga ccctgttgaa gaggttattg ttggtaaaca
     3361 gattttaaga agtttgggac ttttaaatga taagattaaa gttatatcat gccctacatg
     3421 tggtagatgt aatatagact taattagtgt tgtaaatgag gttgaagaaa agattggtag
     3481 tatggaaaaa aatatcactg ttgcaattat gggctgtgct gtaaatggac ctggtgaagc
     3541 cagagaggca gatataggta ttgctggggg taaaggtgaa ggtcttttat ttaaaaaggg
     3601 tgaaatagtt agaatgattg atggtaacaa attagttgat gagttgctag aagagattga
     3661 aaaattatag tatacaattc atatatatat taattgacta gatttttaaa tacttttgta
     3721 ataagttttt atttacagta tttaattatc tagtcatttt ttattttata aacttcatta
     3781 aatgtaagaa acttatattc taatttgact atatatgtat gagttaaatc atattgtaaa
     3841 tttataaaat taaaaaaaga tttaatatag tgaaaatttc catcaatagt attgaaactt
     3901 gatagtaact atgataggat atagttacta gaacagttgg taaattatgt taacatagat
     3961 tttaatgttt tggtaaattg ttttattata agttaataga aaaagaggtg aagaactgtg
     4021 agatataaaa aaatatctag cttacctatt ttagttgttg ctttgctgat tactggaacg
     4081 acaacaacat tttctcttag tacgaccaat tcagaaaaaa caaatgtata cttagcaaat
     4141 acggtaagta ctgtcacaaa gaaggtatca gatattcaaa gtagtaaagg tcttgtttat
     4201 aatgagaata ttccattatt tataaataat agcaaaatac agtatgatga tactccatat
     4261 cattggccta gcaatgttat aagcttaaca aatagcagtg aaaaggcaat tatggattat
     4321 gaaattacat gcttagcata tgataaaaat ggaaaaccac ttgagctata ttgggatgct
     4381 caaaatgtgg cagcagatgg agaagtagga agtgttggat ttagcccagc tggtgtagac
     4441 tatggtattg ttacaggcat atctcctgtg tctccaaaat catattcaca tacttataga
     4501 aaaatgcaac aatcaccacc acaggatata ataagtatgt ttgaaaaaca acaaggcaag
     4561 gcatgggtag aaaattggtt gaaagaatgg aaacaaatgg agaaagaata tgcaaagcaa
     4621 aatgcaatag caccaggtaa gaatcaaaat gatgcctttc tattgtttga taaatggaaa
     4681 caatcaactg gagagcatgg tgtaaagtac attatatctt gtgtaaaaca agtaacattt
     4741 aatgatggta gtgtatggaa aaactcagca tatgagaatt ggttgaaaag ttttcaaggg
     4801 aaggaagttt caaatagtgt attagaaaat tactataagt aatgtttaat gatagcaaaa
     4861 tgagtacgaa tttaattaat gttaatttaa ttaaataagg actattttgt ttaaaaatat
     4921 cagtgttaca aggaaatttt tattgtgaca aaatgatata tatacataag attataagca
     4981 tgtgtcaagt tttgatacat gcttttttat tttgaatata tgttttctat aaatttatga
     5041 ttttttagtt atgtaatttt atatgtgaat ttttagattt ttatatagat agtgaaataa
     5101 tgtggaagtt ttatgtttta aaaattttag ttaaaacatt tattaaaaaa gataatagaa
     5161 atgttttaac taaaatagtt agaattgaaa tgttatacag ctagtataag tactatcaaa
     5221 ttatataaga ttgcttgaag ttaaataatt tatttaaata gaaaacagct gatttaatat
     5281 cagctgtata tgaataaaat tttgaattgt agtgtttagt tatatggata taattcataa
     5341 taagttagca ataaatcaac cattttacca taactctgag taccttcagt aacgccatta
     5401 gcttttaagt aagaattgtt aaactcatta gaaatttcat taattttacc ttcatatttt
     5461 tgccaaaatt cactttcatt ttttaaatct ctacggacag aatctgaaat gccagcagaa
     5521 atatcaacat atgcattata atcaacctta cttaaagcag aagcagtata atttaaagct
     5581 aaaatatatc cagaatagtt aaaatcaata ttggggtgtt ttatagaagt aagatacgct
     5641 ataaaattag cttcatcttc actagcaaat cccctttgat gtgccatttc atgttcaact
     5701 gtacaaggaa tatataagtc aggtattgct atatttacat ttgcctcacc tgtaaaagga
     5761 aagtatatac cagttattcc tgtatagcac attagatttg atgaaataat atacttaggc
     5821 ttagaataac tatcactaac accaggtaat atatccaaga tattatcgta tcctagttga
     5881 gctctattta tgacaccatt ataatctgta ttagatttta caataccatt tttatcttgt
     5941 aatgtgagtt ttctagtttc atttgcttta attacaagga atttatataa tttagtaagc
     6001 tctttaacac tatattgttt tgattggata gaggtattgt tttttaaatt atagttattt
     6061 ataagtgtgg tttctagtgg aatcctatta tagtttattc cccaaagcac tacaaaaa
//
LOCUS       DAGSHT010000194         6073 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGSHT010000194 DAGSHT010000000
VERSION     DAGSHT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.200"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..410
                     /locus_tag="MJB72_003109"
     CDS             <1..410
                     /locus_tag="MJB72_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HBY8264182.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /locus_tag="MJB72_003110"
     CDS             complement(710..1297)
                     /locus_tag="MJB72_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY8264183.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="MJB72_003111"
     CDS             complement(1383..1820)
                     /locus_tag="MJB72_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8264184.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="MJB72_003112"
     CDS             1924..2922
                     /locus_tag="MJB72_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBY8264185.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="MJB72_003113"
     CDS             complement(3042..3968)
                     /locus_tag="MJB72_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBY8264186.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4354..5958
                     /locus_tag="MJB72_003114"
     CDS             4354..5958
                     /locus_tag="MJB72_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8264187.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         2266 a          756 c          945 g         2106 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttagag
      481 atgaggattt atgtgagtat gtaaaagtta aagatagatt taagtaagtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atacaatgat taccttagtt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttttaat aaaagtaaaa
     2041 tattcttctt taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttatgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaaacctcct aaaaaatatt taaatttttt gcattgaata gattacatga
     4021 gatgttattt gagaactaag atacttatat tgtgaataat aatgtaactg tatatttaaa
     4081 ataatacatc atgatttaaa aaattacaat tgtacagata taattgagtt tagatattta
     4141 aatttaacaa cttataatat tttttcaatt agtataaagc ctattaattt gtcttattta
     4201 caatgaatag tatttacttc ttggcaaaca ataattaaaa ataaaacttt tatatgatta
     4261 aactctaaag aaaaagttta taaagaagat aatataatta agcagaaaat aatgaaaaaa
     4321 atttattaaa gtagacaacg taaggagaat tttatgaaag aaaaaattta ttacaatgga
     4381 aatatcatca caatggaaga ttcaatatgt ggagatgcta ttcttataaa agacaaaata
     4441 ataaaaaaga taggaacaaa agaagaagta tttgctttga aaaataaaga tacagaaatt
     4501 atagatttac aaggtaagac attgatgcca tcttttatag attcacatag tcacttgata
     4561 gcttttgcta ctactctaaa gctagttcct ttagaagatg caaccagttt taaagacata
     4621 gtaaaaaaaa tacaggattt caaagagtcg aataatataa aaaaaggaga ctggataatt
     4681 ggattttcat atgataataa ttttttagaa gaaaataagc atccagataa gagtgtactc
     4741 gatagtgcat cttcagaaaa tccaatacta atttcacatg cttctggtca tatgggagtt
     4801 gcaaatacat tggggttaaa acagcttggt gttaccaatg aaacacgtga cccagaaggt
     4861 ggacatatag gtagagtaga aggcagtgaa gaaccaaatg gatatttaga agaaaatgca
     4921 ttttttaatg tagcatctaa aataaaacaa ccatcatcaa atgagatttt taattcaatt
     4981 gaaaaagcac agaacatata tctaagttat ggtattacaa cagctcaaga aggtttaatg
     5041 gaagaaaatc aatttaacat tttaaaagca atggcaaatc aaaataaatt aaaaatggat
     5101 gttgtaggat atgtaaattt gaaaaaaagt aagtctgtag cagataataa tagggagttt
     5161 ataaaaaaat atataaatag atttaaaata ggaggttata agattttctt agatggttct
     5221 ccacaaggta agacagcatg gttgagtaga ccatatgaaa attcagatga tggttattgt
     5281 ggatatccta tatacaaaga tgaagaagta gaaaagttta tagatatttc actaaaagaa
     5341 aagatgcaac tcttaactca ttgtaatgga gatgcagcag cagaccagtt gatagatgcg
     5401 tttgagaaag ttttaaactt aaaagagcaa agtagtgaaa atcatataag accagttatg
     5461 atacatgctc aaacagtaag agaagaccag attgatgata tgaaagtaat aaatatgata
     5521 ccttcatact ttgtagctca cacatattat tggggtgata ttcatatcaa aaaccttgga
     5581 gaagatagag cgtttaagat tagcccactt aaaacaacta tagaaaaggg attaatttac
     5641 actcttcatc aggatacacc agtaatagca cctaatatgc tggaaacagt atggtgtgct
     5701 gtcaatagaa ttacaaaaaa aggtatacaa ataggagaaa atgaaaagat aagcccatta
     5761 gatgctttaa agggtgttac tataaatgca gcatatcaat attttgaaga agataaaaaa
     5821 ggttcaatta aagaagggaa attagctaat ttgataatat tagatgaaaa cccacttaca
     5881 atcgacccta tgaaaataaa agatattaaa gtattgcaga ctataagaga aggagaagta
     5941 ttgtattctt tgaagtagat gaaagttaga tattttataa tctataaaaa atagttttat
     6001 ataataagtt aggtagaatt aaataagatt acagttaaat caacaattat atttttatta
     6061 aattttaatt att
//
LOCUS       DAGSHT010000195         6072 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGSHT010000195 DAGSHT010000000
VERSION     DAGSHT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.201"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            159..1433
                     /locus_tag="MJB72_003115"
     CDS             159..1433
                     /locus_tag="MJB72_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY8264188.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1640..1957)
                     /gene="trxA"
                     /locus_tag="MJB72_003116"
     CDS             complement(1640..1957)
                     /gene="trxA"
                     /locus_tag="MJB72_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBY8264189.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2224..2976
                     /locus_tag="MJB72_003117"
     CDS             2224..2976
                     /locus_tag="MJB72_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBY8264190.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            2963..3844
                     /locus_tag="MJB72_003118"
     CDS             2963..3844
                     /locus_tag="MJB72_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBY8264191.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHVTIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(4070..5425)
                     /locus_tag="MJB72_003119"
     CDS             complement(4070..5425)
                     /locus_tag="MJB72_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY8264192.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2332 a          873 c          790 g         2077 t
ORIGIN      
        1 aactgtagct cattttcgtg taatttaata aacatctcaa caagaattaa ttaacaaaac
       61 cccttataaa aacataacat atattaaaca agctcttgat taaaacttga gcttttttaa
      121 taaaaaacag aatagtttat tagataaggg gtttttttat gcaattcatg gatgtatcta
      181 aatacattga aggtgcaaac cttaaagtta aaactaaaag tggtgaaact gttaaaagaa
      241 tatattttaa caactctgcc actccattag ttttaaaaaa cgtagtggat aacttaaact
      301 gtgagattcc tttgcttact tatattaatg ctccaggaat aatctcagaa aaaaatactt
      361 taaaatatga aaatgtacgc tctactatcc tcaaacttat tgatggagac gaagaaaagg
      421 attctgttat ttatgtcaaa aatgctacag aaggaataaa cttcttagca aaatttttta
      481 aagatgaaaa tccagataaa tttgttatta ccacagcaat ggagcatatg gctaattacc
      541 tgccttttaa agttaatttt cctacaaaag ttgttggaat tacagaaaaa ggagaacttg
      601 atttatgtca attagagaat atacttaaaa attatgctgg taatgtatct ctcgttacag
      661 ttacaggagc tagtaatgtc acaggtataa ccactcctat atatgaaata gcaagaatag
      721 ctcataagta tggtgcaaag atattggtgg atattgtgca agtaattcag cataaacctt
      781 tttcaatgat gccacatgaa tgtgatgagc atatagattt tgttgttttt agtgctcata
      841 aatgttattc tccactaaat ggaggagctc tggtaggacc taaaaaattc tttgaaaaat
      901 ttaatcctgt actatatggc tcaggttcta ccaaatttgt aaatgataac aaaatactat
      961 atgcaaacgt accccaacgt tttgaagcag gatacccaga gtattttgga actcttgcaa
     1021 tgggaaaagc actgaacact ttaaataaaa ttggtctaaa cagaatttca aaatacgaaa
     1081 gagaattatt tttatatgct aaagaagaaa tgagtaaaat acctaatata ataatatatg
     1141 gagataaatc aaataatgta attatcccaa atatatcttt taatataagt aatatgtatt
     1201 acaaagatgt agcaaaatat ttagttgata actttggaat agaaactgga gctggagtag
     1261 taggtgcaga tatttatgta caaaaattat taggtataag tccaaaagaa gcatatgtca
     1321 gatttatgag ggaaaatcct gtaggtcttg ttaggataag cttaggaatg tacaatactt
     1381 ttgatgaggt ggatagcttt atacatgcta ttaaaacttt ggcttttaaa taagtttttt
     1441 ctattctttt ttatcaataa atagagagta gttttgtttt cacaaaaaag caaattatat
     1501 caacagtcaa tttcattagc aattagacac tcatttgtaa ctatacaaac tattttacac
     1561 tatacaaaca aaaaactcat tacaatgagt ttttttgttt atatatgaga tttattttaa
     1621 ttacttcaat ttactttaat tacatatatg attcaatttt gttttttaaa ttctccttag
     1681 gcataaaacc tattaatcta tctacaggtt ttccatcctt aaatattagc atagttggta
     1741 ctgtagatac ttcaaatttc tgagctaatt ctatactttg gtctatgtcc accttcaaga
     1801 agtttgcttt ttcaaccatc tcatttccta aagcttcata tattggtgat agcatcttac
     1861 aaggtccaca ccaagtcgca aagaaatcta caactactaa accatcttta ttttctactt
     1921 catttataaa ttcattatta tttactattt ttaccataat atacatctcc tttattatct
     1981 tcttgatatt aatatactac gacctatata acagttcaat taggcacttt tttgtaaccg
     2041 cctattttta atagatttaa tattatgata aatagattta taagattaaa tatttataag
     2101 actaaatatt tctaatatat tttatttttt tcttaatata gttgacattt tttcccttaa
     2161 attataaaat caaattaata actacatagg tagttactaa tcttttatca aaggagtttc
     2221 tatatggata atattattaa tgaattacaa aatataggat ttactaaata cgaatctcaa
     2281 atatacatat cattattaaa ggaaagccct ttaacaggat atgaaataag taaattatca
     2341 ggtgtaccac aatcaaaagt ctatgaaaat ataacaaagc tattaaacaa taatataatt
     2401 atcaatgttg gaactgaccc tattaagtac ataccaataa accctaatga attattaaaa
     2461 aataagaaaa aagaatttga caattctatg gataatctaa agaatagctt acagatatta
     2521 aataaaagta aaagtgtaga atacgtatta aacataaaag gagataaaaa tatattaaaa
     2581 aaagcaatag aaatgataaa aaatgcaaaa caagaaatta taatttcttg cccttatgaa
     2641 gaaattcttg aattaaaaaa agaactccta attgcttaca aaaaaaatat taatattgaa
     2701 atactacttt tagaaaacaa aagtctgaaa ggtcttgata atattcatat tcatggaggt
     2761 aataaaacta cacttgaaaa taaaatgctt tacaaaggaa ttattttgat tgtagataat
     2821 gatgaaatat tgacaggaaa cttatcagaa ggcagtgagg ccatttcaat ttggacaaaa
     2881 aactctaaca ttttatatat tggagttcaa tacataaaac atgaaattta tatttcagaa
     2941 caaataaaag gaggttataa aaatgaacaa aattaagatt aaacaagttg atgcttttac
     3001 aactatacct tttggtggaa atcctgctgg tgtcgttact gatacctcta acatttctga
     3061 tgaaattaag cagaatgtgg ctaaagaaat gaatttatca gaaactgcat ttgtttcaga
     3121 gtcaaatatt gcagatttta aagtacaatt tttcacacca ggtttagaag ttgatttatg
     3181 tggtcatgta acaataggta ctttccatgc acttttggaa gaagggaagc ttaatactaa
     3241 taagagtata ttttatcaag aaacaaaagc tggtgtatta tctgtagagc ttaagaaaat
     3301 aaataatgaa aatatattta tgatggaaca gaatacacct gaatttgaaa atttagataa
     3361 ttgcagaaaa gaacttgcac aaatgatagg tgtgcatgaa gatagacttc ttgaatatcc
     3421 tattataaag gtaaatactg gactttggtg gcttgtattt ggtctaaaat ctttagatga
     3481 tttaaaaaat atctctccag atttaaataa aatcaaaaaa tttagtggtg ataataatat
     3541 aattggtata actccatttt gtatagaaac tatagataaa aagtgtgact accatttaag
     3601 atctatagca ccatatgttg gagttacaga agaccctgta tgtggaactg ggaacgcttg
     3661 cgtatcatct tatatagtac atcacaatat tttagataaa actagtttta ttggtgagca
     3721 aggtaacttt ataaacagac ctggaaaggt atatgtaaat ataactagag aaaacaatga
     3781 cataacttct gtaaaaattg gaggaagtgc ttatactatt ttaagtggtg aaattatgta
     3841 ttaatcccat acctaataaa aacaaataaa aatactagct aactaaagct agtattttta
     3901 tttgttattt ttatttaata atcatactct aacaaatatt ataattatat atatcataat
     3961 attgtattta gaagtaaaat tttattttta ttattaatta gttataaatc aatctaatac
     4021 aacataatta cagtagatta acctataaaa cataataatt gcttactaat tatttatcat
     4081 cctcaacaag tctacttaat gtcttatcag ataagaataa taatatcgct gcaacaaata
     4141 gtattactgg aattatagta tatactttaa tcatatcaaa tttaagagta aatgcatata
     4201 tatatccata tcctttacca gctataaaag tagcaaatgt ccatactccc ataagaacac
     4261 ctagaagctt cttaggtgca tacttactta caaaagagtt accaagtggt gagaataaca
     4321 tttcaccaag acttaaaaga ataccaaaca tgattatcca tagtatacta gctttagcag
     4381 tttcaggagc tccaacccct cttgaaaatt ctgcaccaac aagcattaag aatgcaagac
     4441 ctaagaatat taatccaaga cctgtctttt taaataaact catatctcct tgtggtctac
     4501 tagcaagttt aaaccataat gctccaagaa ctggtcctaa tactatacat acaagtgaat
     4561 ttaatgagtc aaaccaagca agaggaacat caaatcctcc aacattcatg tttacaaatg
     4621 ctgcaccata atcataaact gcaagataag ttagatacca gaatacccag aaaattacag
     4681 agaagaatga tataagtata atagcccaaa ttctcttctt atccatgctt gtaagaggag
     4741 acttatcttt ttcttcaatt ttttcagcaa ctacaccttc tttaaaaggt ctctttcctg
     4801 cattcccaag gaatctccat ccatatatga accatattgc acctattaca caaagtacag
     4861 ctgctataaa gaaacattga gaaaatccaa gtacaccatt ttttgcaaat gttttaaggt
     4921 aaagaatacc aactgctata gtaccaataa aagaacctat attaacaaaa gaatactgta
     4981 ctgaaaatgc tgtatcaagt tcttcttgac tatcaaaaag ctgtccattt accgcagata
     5041 cgttaccttt aaagaatgca gtaccaattg aaactaagat tatcattgca tttaccatag
     5101 aaactgtagt tgcaatagaa ccaagatagt atcctacccc catgataagc atacctatag
     5161 gtatagtata tctagctcct atccatctat ctgatatata tcccccaatt ataggtgcaa
     5221 ggtatgtgaa tgcaacaagg ttggcttgca ttatagcagc ttgtccttta tctattccaa
     5281 gaccaccatg aactatagct gttgtaagga atatatatat taagtacttt gaagcataat
     5341 atgctgctct ttcaaatgag aaagttgttg agcagatata aaatcccata ggaaatttac
     5401 ccttcttttt tactgttgag ctcataattt taaatccttt cttaattgta tttttatttt
     5461 ttacgttttg tccaaattct ttcttttatg aaagatataa tattattatt ttgtaaaaca
     5521 atttgtaatt attattaaga taattgcgca tttatttgta gagtacatat tctcatggaa
     5581 taaaattaat acacttctta atcataaatt taaattatat aaattcacta ttttcagata
     5641 tagttataaa ttaatatatt ctcatctaat accatgaaaa aaccttcgcc atatgcaatt
     5701 cttatttttt acgtgatagt attttacaaa ctaaaatccc ccttttgtag caacttatgt
     5761 aattacattt tagtgctttt ttaaaggagt ctcgaataaa gcaacatata ttttgttcaa
     5821 ataatgtttt tatttatttt ttaaatttat atattacttc acccaaccaa ttctcaactt
     5881 aaataactaa actactatct attcacagat taggcttatt tcttacacat tagtttataa
     5941 taaacactgt gaatctaaac ataaaaactg ttattttaaa gtaattaccc atatattcgc
     6001 tttgatacta tttattgagg ttatactctt tatgtataat atattattta taaaatacct
     6061 caaaatatat ta
//
LOCUS       DAGSHT010000196         6042 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGSHT010000196 DAGSHT010000000
VERSION     DAGSHT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.202"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..166
                     /locus_tag="MJB72_003120"
     CDS             <1..166
                     /locus_tag="MJB72_003120"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBY8264193.1"
                     /translation="FLYNMVRIIVGALIDVGLKRRAPQDIKYMLESKDRCQSSDTAPA
                     KGLCLWKVTY"
     misc_binding    277..484
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            737..1936
                     /locus_tag="MJB72_003121"
     CDS             737..1936
                     /locus_tag="MJB72_003121"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBY8264194.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            2183..3229
                     /locus_tag="MJB72_003122"
     CDS             2183..3229
                     /locus_tag="MJB72_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBY8264195.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            3337..4500
                     /locus_tag="MJB72_003123"
     CDS             3337..4500
                     /locus_tag="MJB72_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8264196.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(4589..4969)
                     /locus_tag="MJB72_003124"
     CDS             complement(4589..4969)
                     /locus_tag="MJB72_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBY8264197.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(4972..5631)
                     /locus_tag="MJB72_003125"
     CDS             complement(4972..5631)
                     /locus_tag="MJB72_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBY8264198.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         2334 a          648 c          980 g         2080 t
ORIGIN      
        1 atttttgtat aacatggtaa ggataatagt aggtgcttta atagatgttg gtctaaaaag
       61 aagagctcca caagatatta agtatatgct tgaatcaaaa gatagatgtc aatcatcaga
      121 tactgcacca gctaaaggac tatgtctttg gaaagtaaca tattaatcaa ttatataaaa
      181 ctctctttaa aataaaaata aaataaatta tatagttgac ataaatggaa aaataaacta
      241 ttatatattt tataagagag atataaaact ttatatttaa atcctgtgag aaagagagta
      301 acttctatgt gttttataga gagctgagga tggtggaagc ttggcaaata agtattagtg
      361 aaaagaactt tggaggatac taattgaatt tagtatgtta gtaggtaatc tcgccttaag
      421 agaaagagtg gataagttta tcttatcaat tagagtggta acgcggatat atttcgtctc
      481 ttggtttttc caagaggcgt tttttaatat atttaattat tagtgtttat ctggtatgtt
      541 gaacatttac taattgatta gctaagttta tcaataagag tggtaccgcg gatatttcgc
      601 ctcttagtat tttaaaatac taaggggttt ttttagtatt aaaatgattt tatgtgagtc
      661 taaattctca gagtcattca atattaagaa tttctataaa ataaaaatta taatttaaga
      721 ttgggaggaa ttaaaaatga aggaaaaagt agtattagca tattcaggag gacttgacac
      781 atcaatcata ataccttggt taaaggaaaa ttatgaagat atagatgtta tagctgtttg
      841 tggaaatgta ggtcaggaag ataaaatgga agatgtatat gaaaaagctt tacaaagtgg
      901 agcatcaaag gcatatgtgg atgatataag tgaagaattt gtaactgaaa ctatatttaa
      961 agccgtaaaa gcagaggcta aatatgaagg aaaatactta ttaggaactt ctttagcaag
     1021 acctataata gctaaaaaat tagttgaagt tgctcataaa gaaggtgcaa aatatatatg
     1081 ccatggatgt acaggaaaag gtaatgacca agttagattt gaagctacaa ttgcagctct
     1141 tgacccgact ataaaagtta ttgcaccttg gagaatttgg gatataaaat caagagaaga
     1201 tgctatagac tatgctgaaa aacataatat aaaggtaaca gcaactaaag ctaaaatata
     1261 ttcagtagat gctaaccttt ggcatgtttc aacagaaggt ggagatatag agcacctaga
     1321 aaatgaacat aaaaaagatg tttataaaca atgtgtagac ccagaagatg catgtgatgt
     1381 agctgaatat gtagaagttt actttgaaaa aggtgtacct aaaaaggtaa atggtgaaga
     1441 attatctcca gttgcactga ttcataagtt aaatgagttg ggttgtaaac atggaattgg
     1501 tgttatagat atagttgaga atagacttgt tggtatgaaa tcaagaggta tatatgagac
     1561 tccaggagga acaatccttt atgaagcaca taatatttta gaaagtgcta ctttagacaa
     1621 agatacatta catttcaaac aaatggtatc ttataaatac ggagaactta tttacaatgg
     1681 actatggtat tgtaaattaa gagaatctat agatgcattt atggaacaaa ctcaagataa
     1741 tgttacagga acagtaaaag ttaaacttta caaaggaaat ataaaacctg ctggaatatt
     1801 tactgaaaat gctctttatg atgaaggaat atcttctttt ggaaatagtg aactttatga
     1861 ccacaaagat gcagaaggat ttattaattt attcacttta ccattaaaaa tcagagctat
     1921 gaaagctgga aaataataaa ataaattatt taagagtgta taaaaactat ttttaggttt
     1981 ttatatgctc tttttttatt tgaatttatt aacttataca gtaaatagta taaaaaaata
     2041 tattatagtt ccaaatggct taataatgtg aaaatataag acctaaaata taaattttat
     2101 aaatacaata gttattgact ctctcattgt aagagggttt atactgaatc taatgaaaat
     2161 ttatagtgag gagatagatt atatgagaaa aataattgaa attaaacatc attcacatga
     2221 ttatgagtgt atgtggaatg gtatagaaga tttatatata aataaaaccg gtgaatgcct
     2281 tccaaacgat tttttctttt tactgtcagg ttttggttca ttttgttata tgaaaacaaa
     2341 taaagctgat ttgaaaagaa tggtagcttt gggagatgga aggacaaaac agatgtataa
     2401 atttttagca ccaattgttg gatttgaata caaacatcat gagtttaaaa agtatgagca
     2461 agcattaaag aaagcaaaat ctgaaattga tgatggatat ccagttattt taggagcttt
     2521 agatatgtat tatctaccat attataagaa gctatatcat aaagaacata ttccttttca
     2581 ttatatatta atggtaggat atgatgatga aggaatatat ctgtatgatt gtggtagaac
     2641 agaattatta catctttctt atgataaatt acgtgaaagc atgaattgca attcccctgg
     2701 attaagcaaa gaaaatacta tttgcacaat aagaatgaat acttcaaaga ataaatataa
     2761 cattgctaga gaagctattg ctataaagcg tgatatgttt ttaaatccac ctaaaggatt
     2821 tttgggatat aaaggttttg aaaagtttat taaagagttg ccacagtgga acactgaatt
     2881 ggataaagat gattatgata agattttgac caatatggtt atgttttttg gaacagtacc
     2941 aacaattcca aatgcaatca aaggaattaa agaaccagat actgttcaat ttaaaggtgg
     3001 atttgataaa atgagtagaa tgctcaacag tattggggag gaatacaaaa acgactcttt
     3061 tatacaagta gctgatattt ttgataaaac tgctattata attgagaaaa tatccaatat
     3121 tataatagat tacttaactc aaacatgtga tgatacagga caacttccgt tattgttctc
     3181 tgaggtctta gagcatatga aaacaggata tttaatcttg gatgtataaa aaatatattg
     3241 ttgaccctaa cattacgtaa tagtgtaata tgaaaatata gcttgaatat attagtaggt
     3301 attgatggct gtaaaattaa aatttgaggt gtggatatga gaacagtgaa acaagtttct
     3361 gatttaactg gaataagtgt gagggcatta cattattatg atgaaatagg tcttttaaaa
     3421 ccaaataaaa ttacagatgc aggatataga ctttatgatg atgaatctat taaaactctt
     3481 caacaaattt tattttttaa ggagattgat attcctttaa gagaggttaa ggaaataatg
     3541 tcaagccaat attttgataa ggttgaagct ctaaaaaatc aaaaaaaatt acttatttta
     3601 aaacgaaaga gactggatga attgatagag cttataaatc aaactctaag aggagaagga
     3661 aatattgatt ttaaagagtt tgatatgagc gagtattttg atgttttaga agaatttaaa
     3721 agagagcata ggaataaggt aattaaaatt tatggtagtg tagaaaaata caatgaatat
     3781 atagaaagag ttaagtctaa tgaagagaaa attgctaaaa tggctataaa acaatatgga
     3841 actattgaga agtttgctaa agctataaaa acaaatttta gcagtgatat attgaattta
     3901 ggagaaaaat ttgatagata taaaaatgat tgtttaaaag aaaaacatcc aaaattaaaa
     3961 gaattatata gaaagcttgt agaagattta aataggaatc cttcttcaac agaccttcaa
     4021 gaaatagcaa aagagataac agatatatct aaaaaagatt atgaaatttt ctctatggat
     4081 actggtgatg acaactggta ttatatggtt caaaattatc tagtaaatcc tatgtggata
     4141 gaagaagtag ataaaaagta tggaagtgga gcaggtaagt ttattggaca agtactaaag
     4201 acttatctaa gagatagaaa accaaagata aatacattgt atgaaaaact tgtagaagat
     4261 ttgagtagag attgttcttc aagggaagtt caaagcattg ttgaagaaat agacaatgaa
     4321 atgaaaagaa gtaatgaatt ttataaaata gacaatggtg aaagatactt tgattatatg
     4381 tcagagcttt atttacaaga ttctaactat ataaaagtaa ctgataagaa ttatggtgat
     4441 ggtgcatcta agtttattgg agaagctttt aagatttatt ttgataataa taattgttga
     4501 aaatttggaa gtaaaaaata tgcaagtttt ataaaagcac aaaaataagg atgtagatta
     4561 gctccatcta tatccttatt taaagttatt aaaatatata gtttacatca aacttaacac
     4621 tttcaataat atttcccaaa tatgttttta tactttcaac ttcttctaaa gattttaatt
     4681 tattttcagt ataaattgct ttgtccagaa tattaaacct taatcctatt tcatcaaagt
     4741 actcatgatt taaaactttg taccaatggt ttttttcttt attccaactc gtagatattg
     4801 aatcagcttc tttttgagct ttttcccaat cttcattttc tatagatttt tctaaaacag
     4861 atatattgtc tttatatcct tctgtaaaat catatagttt attgttaaca taaaatccaa
     4921 ataatacaaa taagatagtc catagtataa caaatgttgt agatttcaat attaatcacc
     4981 ctttttattt tgaataaata ttttgtcatt ttcatctaaa acacaaataa gaacatcttt
     5041 aaaagtttct atgtgattag attttaaaat acccattaac cagttagtat ctttttgagc
     5101 tatgtctaga tttttattta ttattctacc atccagtata agtgggatag gtaaatgatt
     5161 aaaagctctt ggaggagtac tattgtagct tgatgctggt acaacactta gattaccatc
     5221 agtctccaaa atagcatatt gtacatcttt taggttaaag tatccttgaa tcctaagttg
     5281 ttctaataat tcatctatac taattctttc ttttttaagc tctttatata caatttttcc
     5341 tttatctatt aatacagaag gtttacctga taaaaatgaa cttaattttc tactttttaa
     5401 tgataagaat gaaattagag tctgcatgat tacaagtcca gatattgctg caacaccatt
     5461 tatgattggt atattattat tttccattgg gacagatgca acttctgcaa ttaatagtgt
     5521 tataactaaa tcaaaacagt tcatttcagc tatttcacct tttcccatga ctctaagtgc
     5581 aattagcact gttatgtata atataatact tcttattaat actactgtca tatctatcaa
     5641 ttactccttt aaaaaatatt tacacttagt tagtatatcc tatataaaaa atttaattca
     5701 aagtataaaa aaccttaaat aattggattc aactatttaa ggtttttttg ataattttaa
     5761 tattaaattt tttatggatt ttctgttact tttatttatt ttaataacag attcaacaaa
     5821 actcttttta tctcctaatt ttaagataaa atcaggatat tcttcaaatt cattgagttt
     5881 tattaatttg aaagaagaag atagattttt aaagttaata attttagagt tattcactaa
     5941 tacacttctt tttttaaaat aaatttcttt tttacttata tttagtatag ttaatatttg
     6001 tttaagatat attattatat cttctggagt tctatcaaat tt
//
LOCUS       DAGSHT010000197         6001 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGSHT010000197 DAGSHT010000000
VERSION     DAGSHT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.203"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(129..536)
                     /locus_tag="MJB72_003126"
     CDS             complement(129..536)
                     /locus_tag="MJB72_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264199.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1010..1894)
                     /locus_tag="MJB72_003127"
     CDS             complement(1010..1894)
                     /locus_tag="MJB72_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8264200.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2185..2661)
                     /locus_tag="MJB72_003128"
     CDS             complement(2185..2661)
                     /locus_tag="MJB72_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBY8264201.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            2927..4102
                     /locus_tag="MJB72_003129"
     CDS             2927..4102
                     /locus_tag="MJB72_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBY8264202.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4183..4986)
                     /gene="proC"
                     /locus_tag="MJB72_003130"
     CDS             complement(4183..4986)
                     /gene="proC"
                     /locus_tag="MJB72_003130"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBY8264203.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5026..5610)
                     /locus_tag="MJB72_003131"
     CDS             complement(5026..5610)
                     /locus_tag="MJB72_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264204.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1996 a         1023 c          673 g         2309 t
ORIGIN      
        1 ttagctttag gggtttccag caattcaagt agtattggat aggttctatc ctatctctac
       61 atataggttt ccctatatgc tgactatttt agctactaac tcatgactaa agtcacgagt
      121 gtgcgtagct attttttaat caatatttta gtaaatttta cggtaagtaa aaacctctct
      181 ccttcaaatc ctatagcaaa tatacttaca agttgctttg agaaaataac tcctaatata
      241 gctacaacta tgcatatacc aactattata tttaaaacat tgtttaaaaa ctttagtgct
      301 tgcttttcac cttgtttact acttatatct tgatacattg gaataaaagt agtaactata
      361 gcagtaccta ttgctgcaaa tataatattt ggtatattca tagctgttaa ataactctct
      421 gtatataagc ctgtaccata aattgatgat aatactaatt ctctacccat tccaagaatc
      481 ttagagatta tagtaactat catcaaataa aaagttgctt tagctacctt gctcatttta
      541 tcactccact tctaacatac ctctttttaa tcaagtaaaa tttagctcat tagatataat
      601 tctatcaact aatttgcctt gcttagccct atcaaaatat aattaacacc tatttcttaa
      661 tatatcacaa actacattat tcttcatatt tttttatttc tccaataagt tttacatttt
      721 tcctatataa attgtatagc ttcaattata ttcaaagaaa tggagttgct acccgataac
      781 tttaaaagta aaaaacagat tttattatct ttttaaacta tattctaaca tattttgtcc
      841 atattgagaa attttactaa actattatgt actttattaa ataaataaaa taacagcaca
      901 cctataaaat acaagtgtgc tgttcataaa cagattttat aaaaaaataa ttcctaagta
      961 gagattaaca tgctttctca tactctcata tataatgtgt ctaaaaatat taataaaaat
     1021 gctttaattg ttttaactta cttggatgtc ttaattttct tattgcttta gcttctatct
     1081 gtcttattcg ttctctggta actccaaata cctttccaat ctgttctagt gttttagggt
     1141 catcatcatc aattccaaat ctcattctta aaacttgctg ttcttgctct ccaagacctg
     1201 taagtagttc ttctatcttt tcttttaaat tatgctgttc aacttcgtgt attacaacat
     1261 ctttaaaatc agcatctgat ggtataaatt caactaaact actatcctca tcttctttta
     1321 atggagtatc caaagataca acatttttat ctcttcttaa taattccaac actttgtcaa
     1381 cttctaagcc acatgcatct gcaatctcat ctatagttgg ctctcttaat aatacattta
     1441 aaagttcttg tttctttttc tttacaaaat ttattctctg atgaagatgt ataggtatcc
     1501 taatagtatt ttcgcaatcg tcgatatatc ttgttatact ttgttttatc caccaagttg
     1561 cataagtgct aaatttgtat ccttttttat aatcgtactt ttctacagct tttataagtc
     1621 ctatatttcc tgcttgaatt aaatccaaca tatcaatact atctctttta tatctctttg
     1681 ctatgctcac taccaaccta taatttgaat ttataaattt ttcttttgct actgaagatg
     1741 agttgattat ttccttagcc agctctacct cttctcctgc tgacaacatt ttgtattcct
     1801 ctatttcctt taaatacatt ttcatcgcat tcgatacatt tgatgaaccc acacaaatat
     1861 aattttcata attattttct tttttagtat ccataatcaa accccttcta ttcatagaat
     1921 ttgctaaaac aaaaataaat aatttaaacc tacataatct acaattacat aatatcccat
     1981 tctttaaatg aattgttttg tatattatgt atacaaattt ataaaaacta tacctataat
     2041 attattctac ttctaacagc aatatcctcc ttatatatat aatttatttt attatatgtt
     2101 gttatattct tagttttttg aatgatatat gtctaaaaaa agactgttta ctaaacagtc
     2161 tttttttata tatatgctca atatttaatt ttcatgatga tgattacatc cacatccaca
     2221 attttcatca tggtgatgat gttcttctaa atcttttacc tgttgttcca tttcatgaaa
     2281 ctcttttacc attatatcat aagttggaag agctgcctca gtatcgtatt cttcccctct
     2341 ccaatcagcg tacatcatag catctccatt agttaaaacc aatctacctg acatagttat
     2401 attatccact tcttctaaaa attctatttg tttttgtaaa acttctcctt ctaactccac
     2461 atcttcatct aaacattgca ctatcattac aggtgcataa taatcttctt catctttaac
     2521 atttacaaca atatctaaaa ttccttctgc accttcttca aattctgcca actcaacatt
     2581 tgtatcaatc atatcttgag gtactaataa atcttctatt aaatatttta ttactctatc
     2641 ttttactaat tcttttgaca tttatttttc tccttccgat tctataacat tatcataatc
     2701 atttctatat tatagtatac tacattaatt gttttttcaa tctcttacaa aaattatttt
     2761 ttattatctt ccttatattc ccttttttat tacaaagttg taatcgcatt gatttaaaaa
     2821 atagtttata tttagtataa tagaaatgta gcacatattt ttattcaata tattaataaa
     2881 aaaatatatt gaaaatgtaa tgtatgttaa atctaggagg aatattatga agtttaaaaa
     2941 acaaattagt tctattgttg tgtctactat gcttgtactt gggagtatga acctttctta
     3001 tgcagacggt acaaatgtag ttacacttgg agctaactta tcagagtctc aaaagcagca
     3061 aatgcttaat tactttggag taaaaaaaga ccaagtctta gttttagatg taacaaatgc
     3121 agaagaaaga cagtatttac aaggtgttgc aacagagggt caacttggaa aagttactat
     3181 ctcttgctca tacgttgaac ctactaaaaa aggaaatgga attaatgtta aaacagcaaa
     3241 cttaacttgg gtaactagtt caatgattgc ttcaaccctt acaacagctg gtcttgaaga
     3301 tgcaaatgtt attgctgctg ctccatatcc tgtaagtggt actggagcat tgacaggaat
     3361 catgaaagca tttgaggatg cttcaggaaa gaaacttgat gaaactaaga aagaaattgc
     3421 ttctgaagaa ttggtagtta caggtgactt aggagatgat atagggcaag aaaaagcaac
     3481 aggtgtaatg aatgatataa aaacagaaat tgtaaagaat ggtacaaaag ataccaatca
     3541 aatagcaaat acaataaata atgttgtaaa taattacaat attactatta caccagaaca
     3601 aaaagaacaa attaaaggtg taatgaaaaa aatagctgac caaaattacg attacaatag
     3661 catgaaaaat acactagata atgtatcaga taatgtaaat gaacaattaa agaaattagg
     3721 tgaaagtgtt aagggttctg gtatactaga tactattggt ggatggttta gtggaatagg
     3781 agactggttc tctggtttat tctcaggcgg agataaaaaa gatttgggaa tcttaaataa
     3841 tactaatgat gagtctcttg gctctgatgc tgtaataaat tctacagaag gtgttcaaat
     3901 aaatccacaa aataatacta attctaacaa tgatagtgat acaaatagct caaaaaatga
     3961 tacttcaagt aataatgaaa ataacgatag ttctcaaaca aatgaaaaca acacaacaaa
     4021 taattcagaa aatcaatcaa ataataatga ctcagttggt ttctttgaaa aaataaaaaa
     4081 ttggctttca ggcttatttt agaaattaaa caaataaaaa gaattgccta aaatggaaat
     4141 tttctataaa aggcaattct ctttatttac aataaatttt tattactcac tcattttttt
     4201 agatttttct atacatgcac atattgcttt tatcacagaa gctctaaaac cttcttcttc
     4261 aagtacttta actgcttcta tagtagttcc accaggtgaa cataccatat cttttaattc
     4321 accaggatgt tttcctgtct caagtaccat ttttgcagaa cccataacag cctgtgatgc
     4381 aaacttatat gcttgtggtc ttggcattcc tgcaattact gctgcatctg ctatagcttc
     4441 tataaataaa aatacatatg ctggtgcaga accacttgca ccaattacag cttctataag
     4501 atactctggt actacctcag tatttccaaa agaattaaat acttctgtaa ctgcttctaa
     4561 gtcttctttg tttatatttt tatttatcga aagagaagac atggcttcat ttacaagagc
     4621 tggagtattt ggcatagttc taactatttt tttatcttct cctataatag actctatatc
     4681 ttttatagat tttcctgctg ctattgtaac tataatttta ttgtcatcta taaagtcttt
     4741 tatctcttcc aaaatatcat catatatatc tggtttaaca gctacaatta ctatatcaga
     4801 ggttctagtt acttccttcg agtcttttgt agtatttatt ccaaaatttg catctattct
     4861 atcgagagtt ccttgactat atgctgatgc tataaccata ttaggttcaa ctaattttga
     4921 atttactatt ccccctatca tagcgcttgc catatttcct gcacctatga atccaatttt
     4981 tttcacttta gtacctcctt attgactaat agtaatcgtt aaaatttaag cttctttaac
     5041 tgcatcagat ttttcaaatg gatttggtat acttactacc aaaattttca ctaccttatt
     5101 agtatagttg acccaattat gattgttctt ttcactatta tagtgtattg tgtctcctgg
     5161 atacatttct atttgttcat ctcccaaaaa cactgtaaga gtaccctcta atacatacac
     5221 aaattcctca ccttcatgta cgtaacaaca aatttcctca ctactcttac ttggaagaat
     5281 ttcaattaat cttggtagca ttgttttttc ctttaaacta gatgataaat gataatgtat
     5341 aaatgttgag ttttctacat caaatacttc tttttcataa cttcttaata cagctctttt
     5401 atgctctttt ggcttcataa aaaagtaagt taattctaca tctagtacac ttgctatttt
     5461 tcctaaggaa tctgttgcca cacttgttaa tcctctttca agttgtgata aaaaaccaat
     5521 tgacaagttt gttttttcac tcatatcctt cagcgtcatt tgcttttgag ttctgagttg
     5581 ctttattttt gctcctatgt ccttattcaa aaccatcacc cctaaataat tttaattttt
     5641 tcataatata aatacaaaaa tataaatata ggtactgaac ccatctttat ttcatttaaa
     5701 aaaattttta taagtattac tataatttat caatcttttt ttgtctacat ttttacgatt
     5761 atttacgatt tatttttcac atatgtatat ttaatcttgc caactaaaga tacatttttt
     5821 ataagcattt actctttata taataaaact taagttgttc atgtagtctt cttctggttt
     5881 aattattaaa atttcaaatt atattattat ttctacacat tattacatcc tttagtacaa
     5941 ttataacata attttacaat ttaatgatat ctattcaatg tcttttttta ctctacaaca
     6001 t
//
LOCUS       DAGSHT010000198         5997 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.204, whole genome shotgun sequence.
ACCESSION   DAGSHT010000198 DAGSHT010000000
VERSION     DAGSHT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.204"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(354..1181)
                     /locus_tag="MJB72_003132"
     CDS             complement(354..1181)
                     /locus_tag="MJB72_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBY8264205.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            1787..2242
                     /gene="smpB"
                     /locus_tag="MJB72_003133"
     CDS             1787..2242
                     /gene="smpB"
                     /locus_tag="MJB72_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBY8264206.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            2400..2749
                     /gene="ssrA"
                     /locus_tag="MJB72_003134"
     tmRNA           2400..2749
                     /gene="ssrA"
                     /locus_tag="MJB72_003134"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2902..3150
                     /locus_tag="MJB72_003135"
                     /pseudo
     CDS             2902..3150
                     /locus_tag="MJB72_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3181..3468
                     /locus_tag="MJB72_003136"
                     /pseudo
     CDS             3181..3468
                     /locus_tag="MJB72_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3502..4101
                     /locus_tag="MJB72_003137"
     CDS             3502..4101
                     /locus_tag="MJB72_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264207.1"
                     /translation="MSQNIDFNKSNFYLRLDERLAVPLKYADVSTILKFQKWRGCTFD
                     FSNTKLENLKNIIVHGDNDNYNKLLMVIKKRYDDILDEEFWHIEIEELPYIDREFKSN
                     KDIITTFIHGKYYPKRKAFRHIDFIKNQYPFKEYCSKQEDNSTNEIKIDFYTTKECHY
                     KVWCIENIDIQEHIWCELVNVSLSEEYRKLFGEILDSSK"
     gene            complement(4239..5420)
                     /locus_tag="MJB72_003138"
     CDS             complement(4239..5420)
                     /locus_tag="MJB72_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY8264208.1"
                     /translation="MATKRANGEGSIVKNIRNGVQIGWRASISIGRDEKGKLIRKQFT
                     GKTQKEVKEKLDIYRTKMLLGSIVSADKITFEDWFYTWLFDYRAKDLKPKSFERYEGI
                     YRNYIKDTELGKIKLIDLRATHIQRYYNKLIDVYNKPASTIITLNTRLKPCLTEAEKQ
                     GYIQKNYCKMVTLPSDNKIKEVQILSITDQKKFIDSIDGDKFEILFLLALSTGGLRLG
                     ELLGLKWSDISFDDESLTVNRTLQRVTEINKDGTREKKVIEQYPKTKNSIRTVPIPRN
                     ILVKLKKHKIQQTEQRLLLGDAYINNNYVICNDTGLALNNNRPGKILDSLLKKLNIPK
                     IKFHALRHTYATRLFEAGVPPKTVQTLMGHYDISITMDIYTHVMHNTKQDAVDKINDI
                     F"
     gene            complement(5469..5690)
                     /locus_tag="MJB72_003139"
     CDS             complement(5469..5690)
                     /locus_tag="MJB72_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264209.1"
                     /translation="MLKQARKDKNLTQKQLSKIANISQSYISRLEQDIFINSPTIRQI
                     ISLSKALDISAYKLSNYFIDKESAYNKKR"
BASE COUNT         2207 a          775 c          840 g         2175 t
ORIGIN      
        1 aaactttact attaaaaaaa tacaagattt tagataagtt aatgctaatt ataagtaata
       61 agataagaaa tataatatga cataagggct aggaataaga taaatataat caaagaataa
      121 gatatgagaa taattaccaa gagaaatcat taccttaata tgtttataaa tgttagacta
      181 tgttcctaaa ttgaatttta tatatcaagt ataataacct tacagaatgt ggaaattttt
      241 atttaaagaa tacacataaa catttccaca ctctgatttt ataataataa tctaataaaa
      301 tttatagtga ctttataaaa tcaataatga tttaataaat atttataaaa caactatttt
      361 tgcttagaaa ctttattatt catattatca ttagtaggca ttgctccaaa acttagtttt
      421 tcatacatta cttgagttgg agctagccaa actgtaccat taccagtaaa tgtatttaag
      481 aaaccttcac catttaaagc agaaccaatt aaagatttat ctgattttgt aacagaaaaa
      541 ttaacaccag atgttcttgc tattgcaaag ttaccatcta cctttaattc ttcaccattt
      601 ttcaattcat actctacaat ttcactttga ggtacaacac attccagaac gattacacca
      661 gtaccactta attctatttg gaagaagcct tcaccaccaa gtaaagcaga tgagaagttc
      721 ttttgtgtaa atgcttttac ttctattgta tcagagcaac aatagaataa tcctttatca
      781 acaattatag aatcattatc aagactcata aataaataat gtctaaaaga aggttccaaa
      841 taaacttctc cagtaccttt gtatacaggt ttcatcattg attcattagt aagtgctcct
      901 gatattcctt ttttaaaaag acctccaaca ccaccaatct tagtttcaga agatatattt
      961 cctttataat agtataatgc accagcttct gtttttattg atgaattatt taattttatc
     1021 ttaatttgtc tagctttcaa accagcttct ctagcaaaat ataatcccat tgccattaat
     1081 gggctagaag ctccctcaag tttttcaaat tctaatatct caaattcgct tttgccatct
     1141 gatgcatttt ctaaaatttt tacttttttg cttaaatcca tacaaatttc tccccactat
     1201 gataaaattt atctctaaaa tattataaga tataactcta tattacattt tttttgaaaa
     1261 aatcgctact ttatcatcaa atttttacat atttttaaga agtttttgaa atgtttagat
     1321 ttaagttaag ttatttaagg atgtatagtt atttcaatag atattttgag tattatttat
     1381 catttgtggg tattatattt tatgggcatg ttaaaattag atagaagata gaagatagaa
     1441 gatagaagat agaagataga agatagaaga tagaagatag aagatagaag atagaagata
     1501 gaagatttat aatatgtaat atttttgttt tgaaatttat aaattagtat tactggttag
     1561 agttagttta gtattaagta tattcgaagt agttttttag ctattttcat acatttatat
     1621 tacttaatta tatatagcct aactaactta atatatcagt gaaatattga ctaaaatgcc
     1681 taaatatgtt atcatacaag ttagtcaaat aatttggcaa cattaaatat ttagtaaatt
     1741 tctaacattt atggaacaaa tttctagcaa agaaggtgag atttatatgg ctggtaaaaa
     1801 aatattagca acaaacaaaa gagcaagaca tgaatatttt atagaagaaa cttatgaatg
     1861 tggaattgaa ttaaaaggaa cagaagtaaa atcaataaga caaggcaaag ttaacctaag
     1921 tgatggattt gcatctgtag ataatagcga agtattttta aaacaagttc atataagtcc
     1981 atatgagcaa ggaaatatat tcaatgttga ttctttaaga gttagaaaac tattacttca
     2041 taagcatgaa ataagaaagc ttattggagc aactactata aaggggtatt cacttatacc
     2101 acttagtttg tatctaaaaa acggtaaagt taaagtagag attgccttag caaaaggtaa
     2161 aaaactatat gataaacgtc aagatatggc taagaaagat gcacaaagac gtatagaaag
     2221 agaaatgaca ggtaagtatt aaactagata aaactgtatt ttttagaata aaattcttta
     2281 ttatctagaa cataacattg atttttattc ttaaaagtat tatattatat atacattaaa
     2341 aatttaataa aaatggtgat aaagtttgcg aaaagtctac tggactataa actactcaag
     2401 ggggcgtaaa ggtttcgacg tgggtttgga acttgaggct gcatgtcgtg ttactctggg
     2461 tcacgtaaaa actggggaac tttaaaataa acgcagacga taatttcgcg atagcagcct
     2521 agttgctgct cgtccctctt agtcctcctg ccgactaaga ctggacgtca ttatgcaggg
     2581 aactacttta ggggtgtctc gacctgaagt agattaattg ggactggcta agcatagagc
     2641 ctgccacttg gcgctatgtg aggtgagata ttagtgaagt tggctaagca tgtagatgca
     2701 gcaaggaaaa gatttgcgga caggggttcg actcccctcg tctccaccaa ttttgaaaaa
     2761 agaatgatga aatgattggg attacttgga tttagactga gtaatccctt ttgcattatg
     2821 gaaagacaca caattagcac acaactagaa gcttatgtaa atcaatacaa ttagactaaa
     2881 atttttaggg ggataacaat aatggaaaaa ttgatacaag atatcaaaaa tttagatagt
     2941 tactataata caagttatta tacttttcat ttagatgagc ctttatggaa atctcttaag
     3001 gtgaggaatt ggagtggtgt tccttattat attaaacaac tttgtaatga agttaaatct
     3061 ctaattaaag aaagaaattt aactcaatcg gaaatatttc aatatattgc tgaccccaag
     3121 aaaggaacac attatttttt tgtagcacag caaatacttt cctatacatt tcctaaggat
     3181 gtggcatatt cagatatata tagtatttca tgtggggaaa aagaatttcc aactaatatt
     3241 accattgatg aagatctatt gttttgtgct gttacatgtt ttgtgttgga tagatttaga
     3301 tatgatgatt taaatattaa gtcagtaatt tcatatgaaa aacatatgta tgatataaat
     3361 gattataaac ttactaaaat agaaaacttg gactttaaac aaaatggttt tatatatgat
     3421 aaaaaatatt acttatataa tatttttatt gataaatctc ctttaaatat aaatagtctt
     3481 atacctgcag tttttgaaat tatgtcccaa aatatagatt ttaataaatc aaatttttat
     3541 ttaagattag atgaacgtct tgcagtacca ttgaagtatg cagatgttag tactatttta
     3601 aaattccaaa aatggcgagg atgtacattt gatttttcta atacgaagct agaaaatttg
     3661 aagaatatta ttgttcatgg agataatgat aattataata aactacttat ggttataaaa
     3721 aagagatatg atgatattct tgatgaagag ttttggcata ttgaaataga agaacttcca
     3781 tatatagaca gagagtttaa atcaaataaa gatattataa caacttttat tcatggtaaa
     3841 tattatccta aaagaaaagc atttagacat atagatttta taaaaaacca gtatccattt
     3901 aaagaatact gttccaagca agaagataat tctaccaatg aaattaaaat tgatttttat
     3961 acaactaagg agtgccatta taaagtatgg tgtattgaaa atatagatat tcaagagcat
     4021 atatggtgtg aacttgtaaa tgtatcttta tcggaagagt atagaaaatt atttggtgag
     4081 atactagatt catcaaaata aaaggactgt catattgtag acaaataata ttaagaaaag
     4141 ccatgtattt aaatatatga cttttcttaa tctaaatata atatacatat tatagtattt
     4201 taattactat taataaattt aatttcactt catttacatt aaaaaatatc atttatttta
     4261 tccacagcgt cttgcttggt attgtgcata acgtgtgtat atatatccat agttatactt
     4321 atatcataat gacccattaa tgtctgaaca gtcttaggag ggacaccagc ctcgaataat
     4381 cgtgttgcat atgtatgtct aagagcatga aatttaattt taggtatatt taattttttt
     4441 agtaaagaat ctaaaatttt accaggtcta ttattgttta aagcaagacc tgtatcatta
     4501 caaattacat aattattatt tatataagca tcacctaaca ataatctttg ttctgtttgt
     4561 tgtattttat gcttttttaa tttaactaaa atatttcttg gaataggaac tgttcttata
     4621 gaatttttgg tctttggata ctgttcaata accttttttt ctctagttcc atctttattt
     4681 atttcagtaa ctcgttgcaa agtcctgtta acagttaaag attcatcatc aaaactaata
     4741 tcactccatt tcaaaccaag taattctcct aatcttaaac caccagtact caaagcaaga
     4801 agaaaaagta tttcaaactt atctccatca atgctatcaa taaatttttt ttggtcagta
     4861 atacttaata tttgtacctc ttttatcttg ttatcagatg ggagtgttac cattttacaa
     4921 taattttttt gaatataacc ttgtttttct gcttctgtta gacaaggctt taatcttgta
     4981 tttagagtta taattgttga tgcaggtttg ttatatacat caataagttt attgtaatat
     5041 ctttgtatat gtgtagctct taaatctatt aattttattt tcccaagttc agtatctttt
     5101 atgtaatttc tataaatgcc ctcatatctt tcaaaagatt taggttttaa atcttttgct
     5161 ctataatcaa ataaccaagt ataaaaccaa tcttcaaagg ttattttatc agctgagact
     5221 atagagccta agagcatctt tgtcctataa atatctaatt tttcttttac ctctttttgt
     5281 gtttttccag taaattgttt tcttataagt ttgccctttt catcacgacc aatacttata
     5341 gaagcacgcc atcctatctg aacaccattt cttatatttt taacaataga cccctcgcca
     5401 ttagctcttt tagttgccat ttttgccacc tcttatacag tattaattat atttattata
     5461 ctacataatt atcttttttt attatacgca ctttctttgt cgataaaata atttgataat
     5521 ttataagcac taatatctaa tgcttttgat aatgatataa tttgtcgtat agtaggacta
     5581 tttataaaaa tatcctgttc taagcgtgaa atataacttt gactaatatt agcaatttta
     5641 cttaattgtt tttgagttaa gtttttgtct tttctagctt gttttagcat tagatgtcac
     5701 ccaatattat tatatgtcga agtaagtcag aatattcgtc gtacgaatat ttctactatg
     5761 gtaaaatatg gtaatatata agtataaata actcatgtac ataagattat ttaaaagtag
     5821 aaattttaat tttttaatca aaataggggt gattttatgt gcaaaaacaa gataaaaaaa
     5881 gcaatttcta taaatgaatt aaaaaaaaaa gaataaagag gattatgatt attttataaa
     5941 atttgtaatt aaagttgcag aaaaaaagaa taaatgagat agattcatgt tattagc
//
LOCUS       DAGSHT010000199         5982 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGSHT010000199 DAGSHT010000000
VERSION     DAGSHT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.205"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            66..587
                     /locus_tag="MJB72_003140"
                     /pseudo
     CDS             66..587
                     /locus_tag="MJB72_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
     gene            587..934
                     /locus_tag="MJB72_003141"
     CDS             587..934
                     /locus_tag="MJB72_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264210.1"
                     /translation="MLATLKDIQDLDTKIDTNKTELTSNLETAKTELNNKIGDTTQLE
                     TTDKTSLVNAINEVKTSVDSIETTAEKTSYNNTTSNLTATNVQGAIDEIVAEVRGNRS
                     SIISSINNNLIPM"
     gene            950..2428
                     /locus_tag="MJB72_003142"
     CDS             950..2428
                     /locus_tag="MJB72_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264211.1"
                     /translation="MPPASTFICNRTVKKRRGYYSEQDVFLSPCPYVYGEGGMYESTY
                     YGVFDLSNSKYITVPTSTKYEKTATRVYFISGGSMITSSNGMKQAITLELIPDPNIII
                     NDDLGVISDSCSINYRIPDSNASVKFDVTEKLNGVVISKKNYAVDGNYTLNITDEHLS
                     TLSFNSTNNITIELSTYQGGKFLEKTVTFTKGNTKPKLDISSYNSTFATFTAIDTDNN
                     LSKIEWFIDDVLKETITTDLYLEKTINYELTDNAIHTLKIVATDAENATVEKILSISK
                     EIMPLQTDASLSDISTKLIEIGEGFKNGKTSIINTLALKNIEASLNNTLVELSEKIKA
                     SFDSGDASIEDLTNKLTQANNTISQLNSKYKVASGNSVVNSCVNKYGFYHGSDYLFLF
                     PGAIQINGLNFVPNIFFTTFELIDSGYFRKFFVFACRGIFTQDFVITAHYHRLETYLK
                     DFKVEGEVLKLNERDVYMDNRGIQLPCQRQGSSFKWQAIKFI"
     gene            2445..2738
                     /locus_tag="MJB72_003143"
     CDS             2445..2738
                     /locus_tag="MJB72_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264212.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILEHDIITQLHCIDVPYGS
                     IDYEKYRIIGINIETKEPISEEMPVYVNEEEKRIQELENQLLIAENEKVGGIL"
     gene            2738..2920
                     /locus_tag="MJB72_003144"
     CDS             2738..2920
                     /locus_tag="MJB72_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264213.1"
                     /translation="MNINNIVVRILAERILSGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIRESGVVEGAEPTK"
     gene            3020..3238
                     /locus_tag="MJB72_003145"
     CDS             3020..3238
                     /locus_tag="MJB72_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264214.1"
                     /translation="MKKIIVICILVLMLIIPTMGEIFIMVENIKLEGIEQNPTSCYIS
                     NPGIIGKAKCDAAYFIIAADFINNLKVK"
     gene            3305..3706
                     /locus_tag="MJB72_003146"
                     /pseudo
     CDS             3305..3706
                     /locus_tag="MJB72_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752698.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            3706..4464
                     /locus_tag="MJB72_003147"
     CDS             3706..4464
                     /locus_tag="MJB72_003147"
                     /inference="COORDINATES: protein motif:HMM:NF013661.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8264215.1"
                     /translation="MVDIIKMLTKKKCYPNKNNAKFIVIHETDNEDKGADAKRHAQAL
                     NNGNLEASVHYYVDDKVIYQTLDYKDGAWAVGKSYGTALVAGVTNYNSINIEICVNKD
                     GNYTKARENAIDLTRKLMKDLNISADKVIRHYDAKKKYCPRKMLDNPKLWVDFKDKVK
                     HGEGANKMTKYTIVYEGEVDKVLAQIISWNYKKNECRVCDIKDYVPGHTQNLYVIGGE
                     ACNKIGSITKEKYTAIKGNDRFDTLYKALDFINR"
     gene            complement(4491..4949)
                     /locus_tag="MJB72_003148"
     CDS             complement(4491..4949)
                     /locus_tag="MJB72_003148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264216.1"
                     /translation="MPKTNDERYNIDEFKKIHKEEIIDIEKYREYYDYNFIFYNNMKE
                     MNSIENIRKERARIKSALESKNNPVTPFYITLYAAFLTTFFGIIISELKIYFIIIESF
                     ILIFFIIILGKADKKSIESKEYQIFCMTSLQVLDDIERKKLRERKYKKRK"
     gene            5173..5784
                     /locus_tag="MJB72_003149"
     CDS             5173..5784
                     /locus_tag="MJB72_003149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264217.1"
                     /translation="MLFYFNVTKIDDRGTKLTELVFLISDFVKEHGWELRFYPSFTDD
                     VVAYDLGFKEESDINDTEDYDLLRIDMSRDEKVVKMRDYIEKIDGYEKDLIIVDNYIL
                     PNRYDEHYKTMLLKILNGCKKITIITSDYNEELYSFVKEILDIDIEIKISKNFHDRFW
                     LTDNKGIVVGTSLNGIGKKFCLIDKLKDADLKELKEIIKFEIN"
BASE COUNT         2438 a          629 c         1006 g         1909 t
ORIGIN      
        1 caaaattatt gtataataaa aataacaaac cataaatcca agcattttta aggggaggcg
       61 attatgtgga tttttcagat aaaataaaac aatttagtaa aagaatagag gtgataaaag
      121 gaaatttaac tacagaagaa gcaacaaaaa cagcattaat aatgccattc tttcaaatct
      181 tagaatatga tgttttcaat ccattagaat ttatgccaga atatacagca gatgttggag
      241 ttaaaagagg ggaaaaagtc gattatgcaa tactagaaga tggtaagcca attattttaa
      301 tagaagccaa aaatataaaa gataagctta caaagcatga tgcacaatta tttagatatt
      361 ttacagctac agaggcaagg ttcgcaatac ttacaaatgg aataatttat aagtttttta
      421 cagatttaga agaaaaaaac aaaatggatg aaaaaccatt tatgactata aatttattag
      481 atatagatga aaataaaata tctcacttaa agaaatttgc aaaaacaact tttgatgtag
      541 atgtagtttt taacatagca tctgaatcaa agtatactaa tctgttattt tagcaacttt
      601 aaaagatata caagatttag acactaaaat agatacaaat aagacagaat taacaagtaa
      661 cttagaaact gctaaaacag agttaaacaa caaaataggg gatacaacac aacttgaaac
      721 aacagataaa actagtcttg ttaatgcaat caatgaggta aaaactagcg tagatagcat
      781 agaaacaaca gcagagaaaa caagttataa taatacaaca agtaatctta ctgctacgaa
      841 tgtgcaaggg gcgattgatg aaatagtggc agaggtaaga ggtaatagaa gcagtattat
      901 atctagtata aataataatt taataccaat gtagaaagga tgtgattaaa tgcctccagc
      961 atcaacgttt atttgtaata gaactgttaa aaaaagaaga ggttattatt cagagcaaga
     1021 tgtttttctt tcgccttgtc cttatgttta tggggaaggt ggtatgtatg agtcaactta
     1081 ctatggtgtg tttgatttaa gtaattctaa atatataaca gttcctacaa gcactaaata
     1141 tgaaaaaact gcaactcgtg tttattttat atctggtgga agtatgataa ctagtagtaa
     1201 tggcatgaaa caagctatta ctcttgaact tatacccgac cctaatatta ttataaatga
     1261 tgatttaggt gttattagtg attcttgtag tataaattat agaattccag acagcaatgc
     1321 aagcgtaaaa tttgatgtaa ctgaaaaatt aaatggtgtt gtaatatcta aaaaaaacta
     1381 tgctgttgat ggtaactata ctttaaatat tacagacgaa catttgtcaa ctttaagttt
     1441 taactctaca aataatataa ctatagaact tagtacttat caaggaggta agttcttaga
     1501 aaaaacagtt acatttacaa aaggaaacac taaaccaaag ttggatatat cctcttataa
     1561 ctccactttt gcaacattta cagcaataga cacggataat aatttatcta aaatagagtg
     1621 gtttattgat gatgtattaa aagaaactat aacaacagat ttatatttag agaaaactat
     1681 taactatgaa cttacagaca atgcaataca cacattaaaa atagttgcta cagatgcaga
     1741 aaatgcaaca gttgagaaaa ttttgagcat aagtaaagaa ataatgccac tgcaaacaga
     1801 cgctagttta agcgatatat caactaagtt gatagagatt ggagaagggt ttaaaaatgg
     1861 gaaaacaagt attataaata ctttagcatt aaagaatata gaagcaagtt taaataatac
     1921 attagtggaa ttatcagaaa aaattaaagc aagttttgat agtggagatg ctagtatcga
     1981 ggatttaaca aacaaattaa cacaggctaa taatactata agtcagttaa attctaaata
     2041 taaagttgct agtggtaata gtgttgttaa tagttgtgtt aataaatatg ggttttatca
     2101 tggaagcgat tatctattcc tatttcctgg agccattcaa attaacggac ttaattttgt
     2161 tcctaatata ttttttacta cttttgaatt aattgatagc ggttattttc gtaaattttt
     2221 tgtttttgct tgtcgtggta ttttcactca ggattttgta attactgcac attatcaccg
     2281 cttagaaaca tatttaaaag attttaaagt tgaaggcgaa gttcttaaat taaatgaacg
     2341 tgatgtttat atggataata gaggcataca acttccttgc caaagacaag gttcttcttt
     2401 taagtggcaa gctataaaat ttatttaata aatgaggtga taaagtgaac agagcaaata
     2461 gaattatatg tgaccaaaca ggtaaaatac tcttgcaaac aggagaagca acaggggata
     2521 tattggagca tgatataata acacagttgc attgtattga tgttccatat ggaagtatag
     2581 actatgaaaa atatagaata ataggtataa atatagaaac aaaagaacca atttcagaag
     2641 aaatgccagt atatgtaaat gaagaagaaa aaagaataca agaattagaa aatcaattat
     2701 taattgcaga aaacgaaaaa gtaggaggaa ttttataatg aacataaata atattgtagt
     2761 aagaatatta gcagagagga ttttaagtgg aggtttaaac cctctaaaaa atcgagaatt
     2821 tgaactggat gacgtaacta acacagagta cagaaaagca gtagaggatt atataattag
     2881 agaaagtgga gtagtagaag gagcagaacc aacaaagtag gttctttttt tatataaatt
     2941 aaaaataatt tcctttttta gttatatatt ttttattttt atctataata aaaaaattct
     3001 attaaaagga gattaaaaaa tgaagaaaat aattgtaatt tgcattctag ttctaatgtt
     3061 aattatacct actatggggg agatatttat aatggtagaa aatataaagc tagaggggat
     3121 agaacaaaac ccaacaagtt gttacatttc taatccgggt attattggaa aagcgaaatg
     3181 cgatgcagca tattttatta tagctgcaga ttttattaat aatttaaagg taaaataata
     3241 aattgcacta aaaagggctt agttaaatct aagttctttt tttaatacaa aattaggagg
     3301 atgtatgaat atatatgatg gaaaattaag ttcaatcata gcagtaatag gaacaatatc
     3361 aacatggtta tttggagtat gggatttggc gttgataggt ttaataactt taatggttgc
     3421 agattattta ctaggtatta ttaaaggtta tacattaaaa gaattatcta gtacaaaagg
     3481 atttaagggt ttagctaaga aaagtggaat tctaatagtt ttaattgttg ctgttgtatt
     3541 agacagactt ttgagtaatg gaacataggt atttagaagt ctagtatgtt atttctatat
     3601 agcgaatgaa gcactaagta tactagaaaa ttgtagtgta ataggattac cagttccaca
     3661 acaaataaaa aaggcacttc aacaacttag agaggagagc aaataatggt agatataata
     3721 aaaatgttaa caaagaaaaa gtgttatcca aacaagaata atgcaaagtt catagtgatt
     3781 catgaaactg ataacgagga caagggagca gatgccaaaa gacatgcaca agcacttaac
     3841 aatggtaatt tagaagcaag tgtacattac tatgtagatg ataaagtaat atatcaaaca
     3901 ttagactata aagacggagc ttgggcagtt ggcaaaagtt atggaactgc attagttgca
     3961 ggagttacaa actataatag tattaatata gaaatatgtg taaataaaga tggtaactat
     4021 acaaaagcaa gagaaaatgc aatagactta actagaaaac taatgaaaga tttaaatatt
     4081 agtgctgaca aagtaattag acactatgat gctaagaaaa aatattgtcc tcgtaaaatg
     4141 cttgataatc caaagttatg ggtagatttt aaagataaag taaaacatgg tgagggagcg
     4201 aataagatga caaaatacac aatagtctat gaaggagaag ttgacaaagt tctagcgcaa
     4261 ataattagtt ggaactataa gaaaaatgaa tgtagagtat gcgatataaa agattatgta
     4321 cctggtcata cgcaaaatct ttatgtcata gggggagagg catgtaataa gataggttcc
     4381 attaccaaag agaagtatac tgcaataaaa ggcaatgata gatttgatac actttataag
     4441 gcattagatt ttattaatag atagattaga aggtagcaac tagttagttg ctactttctt
     4501 tttttatact ttctttctct aagctttttc ctttctatat catccaacac ctgtaatgaa
     4561 gtcatgcaaa atatttggta ttctttagac tctattgatt ttttatctgc tttgcctaga
     4621 ataataatga agaaaattaa gataaaactt tcaatgataa taaagtatat ttttaattca
     4681 ctaattataa ttccaaagaa ggttgttaaa aatgccgcat acaaagtaat ataaaatggg
     4741 gttacagggt tatttttaga ttccaatgct gacttaattc tagccctttc ttttcttata
     4801 ttttcaatag agttcatttc tttcatatta ttataaaata taaaattata atcataatat
     4861 tctctatatt tttctatatc aattatttcc tctttatgaa tttttttaaa ttcatcaata
     4921 ttatatctct catcattagt tttaggcata ttaaaaatcc tttcttatca tctatatttt
     4981 gtaaataata tactttaatt tatagcatat ttaagaactt atatcagcaa tttgtcgaat
     5041 gattctagca aaaataaagc ataatgatag attcattttt tacaaggtat tggattttgt
     5101 gaataaataa tataacagca tctttattta tcttttattc taatatacaa taaattaaaa
     5161 aggagtattt atatgttatt ttattttaat gttactaaaa tagatgatag aggcaccaaa
     5221 ttgactgaac ttgtgttttt aatttctgat tttgtaaaag aacatggatg ggagttacgc
     5281 ttttatcctt cgtttactga tgatgtagta gcttatgatt taggttttaa agaagaaagt
     5341 gatattaatg atacagaaga ttatgacttg ttacgtatag acatgagtcg agatgaaaaa
     5401 gtggtaaaaa tgagagatta tattgaaaaa atagatgggt atgagaaaga tttaataata
     5461 gtagataatt atattttacc aaacagatat gatgaacatt ataaaacaat gctcttgaaa
     5521 attttaaatg gatgtaaaaa aataactata ataacaagtg attataatga agaattgtat
     5581 agtttcgtaa aagaaatttt agatatagac attgaaatta agatttcaaa aaattttcat
     5641 gatagattct ggctaacaga taataaagga attgttgttg ggacatcttt aaatggaata
     5701 ggtaaaaaat tttgtttaat agataaattg aaagatgcgg atttgaagga attaaaagag
     5761 ataataaaat ttgaaataaa ttagaagaga aatttattaa tagtagttat gtaattatta
     5821 aataatatct gatatagaaa gagaggcgta gtgttgagca aaaaaaatta tttgagttat
     5881 gaagaccaat tcaaaaaaaa tctaaatcaa gaagaaataa gcaggattga aaatgttgag
     5941 ataaggaata ttaaggcaaa atattggaat ttaatgaaag aa
//
LOCUS       DAGSHT010000200         5951 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGSHT010000200 DAGSHT010000000
VERSION     DAGSHT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.206"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(21..683)
                     /gene="zmp1"
                     /locus_tag="MJB72_003150"
     CDS             complement(21..683)
                     /gene="zmp1"
                     /locus_tag="MJB72_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBY8264218.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(783..868)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1053..3971)
                     /locus_tag="MJB72_003151"
     CDS             complement(1053..3971)
                     /locus_tag="MJB72_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBY8264219.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIQEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPIVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(4089..4174)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4301..5479)
                     /locus_tag="MJB72_003152"
     CDS             complement(4301..5479)
                     /locus_tag="MJB72_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY8264220.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT         1774 a         1022 c          665 g         2490 t
ORIGIN      
        1 attttaaatt tattaaaact ctatttagct aaattttgca aaaagctata agtttgtgga
       61 caagctgatt ttaacttact atttgtatct tgatttaaat agtaatatgc aaatgattct
      121 gcaaaaaatt cttctggata tacacctaag tagtttacat tacctaaaga acgaccttct
      181 ttagcaaata tttgtttgaa ttgtgctgat ttagatatat catttaatac tatatggtct
      241 attgcatgtg ctgtttcatg taattctaaa tttattgaat catgcccttt acctttgtta
      301 ctaaatccta ttctaagagc tactgtactt ccacctaaac ctggaacatc atcccaagtt
      361 tttccagtac cttcccatcc cttaggaact acacctttta aataagcata ttctttttca
      421 tctgttaact ttccacttaa taattttatt tttaagttat tttgttttaa tgcatttaaa
      481 tactttccat ctatattagc taatctattt accatagcat tagcttcgtt tttatcgtaa
      541 tttccagttg gaaaaactac tatttgacta agtgtgtctt tattttgttg tatagtagta
      601 ctatctgcat gtgcacttac tggaactgaa cttattaaga atattgatat tatagctatt
      661 aataattttt tacttggtct catattattc cctcctttga gttaggccgg gagtaataaa
      721 tttcaaaata aaaaagatac ccaagagtat ctttgtatat acaaaaagaa atttttactc
      781 ttttcggcaa ctggctggca tggtttacta aactttagcc cctatagctt tgcgtgacta
      841 ggtttcccta gttttgcctc atttaatttt atatttatat tatagcttat atctttttaa
      901 ttgtctatat ccctttggaa cttttttcta aaaagttatt atttttttac ataaatcttc
      961 aaaaaaaaat atcttctaac aatattttca ttattaaata ttgttttaga agatattttt
     1021 taatataact tatattttta taatgttagg gtctaatttg tatttttatt tcttcttaat
     1081 acgataagtc ctacaatagc tcctataacc aaaagtattt ctccaataat tgtagtacta
     1141 tcccctgttt ttggtggtac tggtggattt accaaagtat catctttaac tttattattt
     1201 ttttcttctg acactgatgg ctttactact tctggtattg gaataatctg tttaacagga
     1261 gtttctatca tctcttctga tggtggtact ggcggattca atacctctgg tattggcgga
     1321 tttattatat cttctgatgg tggtactggt ggatttataa ctatagtatt ctctgtatca
     1381 gatgaacttg atggtttcct tggtttatct gtgcttggtg gtactggtgg atttactatt
     1441 ggttcatctg tatctggagg tattggtgga tttactggtg gattttcttg ttttagattt
     1501 tctatattcc caatatctac tatcttacca atatcttcat taccaatttc aaccttaact
     1561 ttatccttca gtttttcata tccacgtgga gccattatct ccactaaata atatgttcct
     1621 gggtttttta cactagattg aattatacca tcttcatcac taactaaatc cttaactact
     1681 atatttccaa atgcatcttc taaattgaac ttagcacctg aaagtacatt tttatctaaa
     1741 gcatccactt ttttagcttc aaaagcataa ccttgcttag tagtctctac tcctggagga
     1801 cttgaaccat taatctgagc cacatttcgt atagtctccc caacttttac atttgtgaag
     1861 tttgcatcaa atgtaaccca actttgcaca ggttcccctt cttttatctg tttaaattcg
     1921 tatttattta ctaatcttat tgtatttttt ccatctccag cttctttttt aagttcaaga
     1981 ttatcagaat attttatatt agatatttct agaactgagt ttacttcatc taaaagattt
     2041 acttgtcctg catcataaac tccataagtt gacatattga ttttgtattt aacaatctgg
     2101 ttatcactac tgtttaaaac ttttttatca acttcttttt ttgcgcttat taatccagaa
     2161 tttttcttaa agtttatctc atcattttta tcaatttcac tattatttgc atcaagaaca
     2221 gctctattta tatagctatc ttcaatacta attaccttta atgtataagc tataaaaaac
     2281 tttgattcca cttcttcttt actgagatta attgtaaatc cagttataac tttatctact
     2341 tgcccacttt catttctttt ttcttctact ttatatgaaa tattattggc aattttttct
     2401 ttactccaag gagtccagct cccattcatt ggaatttcta caactctaaa atcatgccca
     2461 gcttctcctg aagtagttaa tctcatgcct tctggtatat tatctgttat aacaacatta
     2521 cttattggtg atttatcatc atttattgaa atctgatagt ttattacttg accaacttga
     2581 gtaatatttg ttgctcctga atgtccttta gtaagaagat aatcatctcc tctatcaaat
     2641 atacttacag tagcatcatc ttcttgtatc ttcttaccat cagattcaat cctagctaca
     2701 ttcttatatt tattaccatg ttttgcattt ttgtcactga ttctagcctt atacttaaca
     2761 gtatacgatt tatctatatt tccaaaatta actgaaatag aatttttact ctgttcaata
     2821 ttaaaatcac taacttcttt tccatcactt gttactacac ttacagaacc atttatcaat
     2881 gtcaatcctt caggaagctc atcatacaaa ataatgtctt ttaactcttc ctgcttttca
     2941 tttacttgta tactgtaatt tatatcttta tttttaactg gcataaatac agtagttgat
     3001 ttttttcctt caacaagctt tttaagtgtt cctttagcat ctggaacatc cgttttatct
     3061 ccaggtttta tttctacatc tatatctcca cttccacttt ctatttttcc agtagtttca
     3121 atatgtattt ttgcattagt atcttcatca acctttttaa catatgcttc caaacctatt
     3181 ttaccttgca ttacactttg gccattaggt aattctctaa atgtaagtgt atactctcca
     3241 ttactttctg ttttatcaaa gcattcactt gtataatcta aattaacttt attgaataca
     3301 tctggcacct taaaagttat tgaatctcct gcttgtattc cttttgatgt tggagtataa
     3361 gttaaattaa atgatatttt tttcattgac tccgtttcta ttgtattgtt attaattttt
     3421 acaacaaatc catagtcttc attttcttca ttaagttctt cttctaaaac tttcaataaa
     3481 ttttcatctt ctaatagatt atcttcctct ggagtttttt ctgtattttt attgtctagt
     3541 ggtttttcat cttctaaagc ttgttctgac tttttattat ctgttgactt atcttcttct
     3601 tgtatctgct ctggcttttt attatctatt ggcttttctc cttctggagt ttgctctggc
     3661 tttttattat ctgttgactt atcttcttct ggggtctgtt ctggtttttt attatctgtt
     3721 ggtttttctt cctctggggt ctgttctggt tttttattat ctgttgcctt atctttctct
     3781 ggagtctgtt ctggcttttt attgtcgctt tccttctcct ctccagttga tgaattagtt
     3841 gaaccacttt gaatctgact attctctcct aattctgaag cataagatac agttggatat
     3901 attgtagaca ccaccatact taaaatcata attaaactaa ttaataatgc ctttctattt
     3961 cctttcttca tgtaaatctc acccctttag gttacctata atttataatc taatcttcca
     4021 ttaatattgt atataaagta atttttacta aaaaattata caaaaaaagc tatgaaaata
     4081 aataaattta tattcatagc cggttgcacc actgactcag tatatccata aggtgcccaa
     4141 taatagaata tacttcaaca tctctataat atttcataaa aacaattaaa attgctttat
     4201 ttatattaaa aatacaatta tatacttcta ttatattatg cctctatact attgtctatc
     4261 ctttttacat tttttacctt atgatgagat taattaataa ttaaataaaa tccttatcta
     4321 caacaattac acaattatat tcaggattta tctccttaac taattttgtt atatcttctt
     4381 ttaagtcatc ttctgacatg tttttggaaa attcggaagg attaactact atatcaaata
     4441 ttaaattctt cttgcttcct tttccaacta ttctaaaatc atgcatagat tttacaagac
     4501 tatttttctt aagtatttct tcaagttctt ttttggctga cataacttct ttatcttcaa
     4561 gacatattgg gtccatgtga attactagat atatatctaa tttttcagat atttctctct
     4621 ctgcagtatc tataatttca tgtatttcca ttatatctat gttggatgga atttctgcat
     4681 gtatagatgc aatacatcta ccaactccat agttatgaat aattaaatca tgtgtaccta
     4741 ttatatattt ataagataac aatagctcta taatagaatt taccatttct tcatccggtg
     4801 cttcccctaa aagaggattt atagtatctt ttacaagaga gaaaccagca taaagtatca
     4861 ctaatgatac tacgatacca acatatccat ctatgggaaa attagtaaat ctagctacaa
     4921 taaatgatat aactacacaa cttgaagtaa atacatctcc taaagcatca agtgaaactg
     4981 cttttaaggc tgaagaatca attttatttc ccataaattt attaaaacga ctcaaccaga
     5041 ttttaatcat tatagatgca attaaaagta taagtggtat tacttcaaat tttacaggaa
     5101 ttggatttac tatcctttct atagatgact ttacaaattg aagtccaacc aacatcacca
     5161 taaatgctac tattaatgct gataaatact ctattcttcc atgaccaaaa gggtgttctt
     5221 tatcagctgg cttacttgct aatttaaaac ctatcatagt tattgctgaa gatgccatat
     5281 cagaaagatt attaaatgca tcagccatta tagctatact agatgtgagc attcctatga
     5341 atacctttat cacaaataaa agtaaattgg ataatatacc tactacacca gctacataac
     5401 catacttatt tctaacatct gtattttgaa cgttttcgct atctctgata aatgttttga
     5461 ctaagatttt ggaaaacatg aaacttacct ccctgccctt tattttatta acaaatttta
     5521 taatctatat ctttatttat gataacataa tttatatata aaattcttaa aaatcactct
     5581 acttcagtaa aatattattc tatatatcta tttagatacg aattttatat ttagtaaagt
     5641 ttttatggct tagtgtctgt ttaatagatt ctatataagt ttttacaaag attatttatt
     5701 atgataatat catttttccc aagtattact tagaatatga tttttttgtt ttgtctgtac
     5761 atattttaaa tttatactat atttatctat attttataac cttatgatac actataacta
     5821 taagttttta aatggaaagg aatgaaataa atcatgtttt ttatcagtta tatttacaca
     5881 aaatcaccta ttgaatttga tactcctttg caaaaagagg catacaaaat attacaaaaa
     5941 ttagatattg a
//
LOCUS       DAGSHT010000201         5951 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.207, whole genome shotgun sequence.
ACCESSION   DAGSHT010000201 DAGSHT010000000
VERSION     DAGSHT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.207"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..592
                     /locus_tag="MJB72_003153"
     CDS             <1..592
                     /locus_tag="MJB72_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY8264221.1"
                     /translation="LSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNA
                     DLSGASFRFCDLSNSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYK
                     KCFNFRMVQLLIPSDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIY
                     RVGETIVAEDFNENRWKESTTGIHFFLTREEAIGYL"
     gene            complement(720..1916)
                     /locus_tag="MJB72_003154"
     CDS             complement(720..1916)
                     /locus_tag="MJB72_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264222.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1919..2944)
                     /locus_tag="MJB72_003155"
     CDS             complement(1919..2944)
                     /locus_tag="MJB72_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY8264223.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(3016..4146)
                     /locus_tag="MJB72_003156"
     CDS             complement(3016..4146)
                     /locus_tag="MJB72_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8264224.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            complement(4740..5135)
                     /gene="tnpA"
                     /locus_tag="MJB72_003157"
     CDS             complement(4740..5135)
                     /gene="tnpA"
                     /locus_tag="MJB72_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBY8264225.1"
                     /translation="MKLDSNNHSVFLLHYHLVMCIKYRRKVIDDSISDRLKDIFVDIA
                     PNYGVVLIEWNHYVDHVHLLFKAKPNTEISKLLNAYKSASSRRIKNEFPSIRKYLWKE
                     YFWSKSYCLVTTGGATLEDLEQYIENQEE"
     gene            complement(5300..>5951)
                     /locus_tag="MJB72_003158"
     CDS             complement(5300..>5951)
                     /locus_tag="MJB72_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264226.1"
                     /translation="YKEYIETWVHEIKTPIASSKLIIENNSNEITKKIDTQLDKVENF
                     VEQVLYYSKIDNVNKDYIIKKLNLEEVVNEVIKRNYRDFINKNIKLELGEINKTVYSD
                     SKWVEFILNQIVVNSIKYIKSKDGLIKISAKRVENSIVLTIEDNGVGIVERDINKVFE
                     KGFTGENGRVFGKSTGIGLYLCKKLCNKLGLGLILESKVNVGTVIKVSFPIDYNLF"
BASE COUNT         1957 a         1041 c          611 g         2342 t
ORIGIN      
        1 cttaagtaat attgactttt cgtggtctga ccttatcaat ttaaatttag aaaaagcaga
       61 tttagaaaac tctattttta ataattctta tctttcggat tgttcattaa aaaattctat
      121 attaaaaaat gcagacttaa gtggtgcaag ttttagattt tgtgacttat ctaactcaga
      181 tattagagga gctaatttag aaaattcaaa tttagaacat gctactttaa atggagtaat
      241 atcagatgaa agtacccgtt tttttaaatt gtattgtcct gaaacaggag catttatagg
      301 ctataaaaaa tgttttaatt ttagaatggt acaattatta atacccagcg acgcaaaaag
      361 agtgtctgct acatcaaacg cttgtagatg tgataaggca aaagtactgt caattactag
      421 tatagatggt aaagaatctt ttaaaagtgc tcgtgcatat gctgatgaaa attttatata
      481 tagagtagga gaaacgattg tagctgaaga ttttaatgaa aatagatgga aagaatcgac
      541 tacagggatt cacttttttt taactagaga ggaagctatt ggttatctat aaaatgctaa
      601 agttttataa atatataaat acataaacaa aataacaaaa gagtatagat tgtaatttca
      661 aaaaaattat aatctatact cttttttcta tagcacataa gtacatttta tttcataaat
      721 tataataaat tatttatatc tgcactattg attatttgag aaactttatc tatatgttct
      781 atcttactat ccaaactgtc ataatacttc ttactctcat ctctatttct aaaagaataa
      841 acttttttat ccaattttaa agcctctttt ataatatcta tattcctctt atttgtctct
      901 cctacactaa atcctgtatc tattataatc ttggaatcat ttaaatttct aatagccaaa
      961 tcgaaactct catcacttat aggctcaaat ggattttctg taaacatctt tatccccata
     1021 gttctaccaa tttcataatc aatatcattt tcatgtatta ttccagaata cagacctata
     1081 ccttttttag taaaagctct ataaattggt gttgctttac ccccaccacc aattataaaa
     1141 acttcatttt tagatttatt actaatttct actgaaccta aaagactatt aaagttttta
     1201 tcatccaatt cataaagcga atttataata tcctcatcta ctacatcttc aggctgacca
     1261 taagctatga ctttattatt ttttataaga gcaactttat cacaactttt taatgctatg
     1321 tctatttcat gcaatgaaag tattactgat attgactttt ctttacttaa ttttttcagt
     1381 atgtttatga gttccaatct atgttttata tccaagtgag ttgtaggctc atctaatata
     1441 attatttctg gttcttgaac aagagccctt gcaaccaaca ccttttgttt ttctccatca
     1501 cttagttcat caaaatatct tttctttaaa tgtaaggcat ctacagactc caatgcttca
     1561 tctaccatta ttaaatcttt cttagataga ctcccaaaaa atcctgtata tggatacctt
     1621 cctatattga ctatatcttg tacagtcatt aaatcaccta ataatctatt tgtaagtact
     1681 aaagccattt tttttgataa tgcttttctt gatatacaat ttatgtttac accattcaaa
     1741 tatatttcac cttttattgg tgatataagt ttagataagg accttaatag ggtagttttt
     1801 ccagctccat tacttcccaa aagacatagt atttctccat tttttacttc tacatttata
     1861 ttacttataa caactttgtt attgtatcct acacttaaat tattagtttt caacatgtct
     1921 aagccctatc cttccttttc accaataaca ttactacaat tggagctcct aataaagatg
     1981 ttatcgtact tattggtaat tctactggtg ataaaattac tcttgatatt aaatcacaaa
     2041 gagctgtaac tatactgcct aaaaatatta ctactggtat caaaactcta ttatctgatg
     2101 ttttaaatat catttttgaa atttgtggca ctgcaagtcc aataaaccct ataggtcctg
     2161 caaatgctga tactattgct gctaatatac tagatataaa tactatcaaa actctaaaaa
     2221 atcttatatt tatgccaata ctttttgcat actcttctcc tagtaaaaaa gcatttaaag
     2281 gttttattat gaatacagtc atcaaaagac ctaatatacc aactattata agtatttcta
     2341 ccttttccca tgtaaatcca gaaaaacttc ccatagacca catagtaaat tcttttaatt
     2401 tatcactatt tgcaaatgat tgtaacacat ttgttatacc actacataca tatccggtca
     2461 taattcctat tattaaaaga gttgttatat tctttacctt atttgcaaat aaaatgacta
     2521 tagacattac aattaaagct cctataaaag ctgctacaaa tatactcatt gaatttacac
     2581 tctcaaatcc caatgtgtat cctcccaaaa tcatgagagc tactgataaa gtagaacctg
     2641 aagatactcc taatatatac ggttcaacta ttgggttttt aaaaaatatc tgtaataaaa
     2701 tacctgatac tgctaggcat gctccaccta ccatggttgc aagtactcta gggattctta
     2761 tattgtatat tattttataa tgttctgtat tatccatacc tcttaataca acatctatta
     2821 cttctttaaa ttcaattttt acagagccaa atgctacaca cactaaaaaa gtaagtatca
     2881 tacttataat taatactata aatattgttg tattccttat tctcatttta ttgataaaat
     2941 ccatttgtat actcctctta gcaaagctat attaactaaa atctataaaa cctatctttt
     3001 tgtgtcctct ctgtactatt tatcataatt tatataatgt ttgatttttt cacctgtagt
     3061 acctgggtgg aacatctcaa ctaaatctac tattaattcg tccgtcttat ctactgattg
     3121 ataataatct ggagcaaaca cccataggtt cccgtttttt acaacatcca aatcagccaa
     3181 tacaggtgca ctagatttta tagaatctaa atttggtgca taatctgaac ttgatgagta
     3241 tacaaatata tctgcatctt ttgcaacttc atgcaattgt tcaatactta ctcgcccttc
     3301 atttgaatct attgaagcac atgcatttat tccaccagcc atttttatca tattatccac
     3361 atatgaatct ttaggagcta tataagcact tccttcatat acagatgccc atacaacttt
     3421 aggtttagct ttgtttttta tttttttact tacttcttct accttttgta cagttttatt
     3481 tagttcacta gtagccatat cttctttatc ataaaatgca gctattaaac ttgtccactc
     3541 cattcttcca aatggatttt gctctttata ctcattgtct acaacataat taagccctaa
     3601 ttcttttaat ttgtcaataa attttgtatc agcctctgat aaaccactac tcactatagt
     3661 taagtcagga tttaagtcta ctattttttc ataatctggt gctaaatttg aaccaacgta
     3721 atgtattttt ccatctttca ttaaatcttt aatttcttta atagtccagt actgctcttc
     3781 tgttgttact gcttttatac tatctagttc atctattgtt ctcagagaac aagcctgtgt
     3841 tgtagatgct attaatacat tgtccacagg cgttctaact atattagcat ttttatattt
     3901 ttcaggaatt ttaatcttct ttgggattaa aatcagttct cgattatctc catcagttac
     3961 ttttttaatt ccatcttcta aatcttcaac actaaatcca ctagcatatt ttaattctaa
     4021 tttcttacca gtactagctt gtttattctc tttatcactt ttactacacc ctataagacc
     4081 tcctaccata aaaattacca tagcaattaa aaaaaactgc tttaaaacat ttgatttaaa
     4141 tttcatctct ttcactccaa tatcttattt tttatgagtg gctaaaaaac tttaataaga
     4201 tatacctttt aaagtatatc ttattaaagt tatattaaac catctttaac aagtataact
     4261 ttttaataaa attatagtga attttgattt cacatgcaat taactactta tacacttaac
     4321 tacctacgac aacgtttgaa gtggcatctt ctttgtcaaa atagttattg ttattagtat
     4381 ctccaagcta ataggatagt tcccaacact tacgattagc atctgagtca ctacgttggc
     4441 aaactaatag aaatataaca tttatttgat gatgctttag atacagtaca actctatcaa
     4501 attgcctatg ttgttttatc ctaatcattg attttaattt aagcagtttt atatacccat
     4561 ttctatatgc acaatatctt ttgagtaacg tgttgacaaa ctattttggt tagtagtata
     4621 gctatgatag ttaattttct ttgatagata agtccgttac atccaaaatt cttaggaaaa
     4681 tattcctttt attcactgtt tgtataaacc tgtacttata agctcttaac atattatcac
     4741 tattcttctt gattttctat gtattgttcc aaatcttcca atgttgcacc accagttgtt
     4801 actaaacaat aacttttact ccaaaagtat tctttccata agtatttgcg gatacttgga
     4861 aactcattct ttatccttct gcttgatgca gatttatagg catttaataa cttggaaatt
     4921 tctgtattag gctttgcttt gaataataag tgaacatggt caacataatg attccattct
     4981 atcaacacca ctccataatt tggtgcaata tctacaaata tatcttttag tctatcagat
     5041 atagaatcat ctattacttt tcttctatat tttatacaca taactaaatg ataatgcaat
     5101 aagaagactg aatgattatt actatctaat ttcattataa ttacttccct tcagctttta
     5161 ctactaaatt aatgatataa tacgttttta tgaaaatcaa gtaaaaaaaa gaacctatca
     5221 attatgctta catccttaac tttcttaaat aaatacaaaa aactgacaac taaaaattgt
     5281 cagtttttat aactatacac taaaataaat tgtaatctat agggaatgat acctttatga
     5341 ctgtaccaac atttactttt gattctaata ttaaacctaa acctaactta ttacataact
     5401 ttttacatag atataatcct attccagtac tttttccaaa tactcttcca ttctctcctg
     5461 taaacccttt ttcaaatacc ttattaatat ctctctctac tatacctact ccattatctt
     5521 ctatagttaa cactattgag ttttctaccc tttttgcact tatttttatt aaaccatctt
     5581 ttgactttat atattttata gaatttacca caatttgatt taatataaac tcaacccatt
     5641 tcgagtcact atatacagtc ttattaattt caccaagttc taattttata tttttgttta
     5701 taaagtctct atagtttctt ttgataactt catttacaac ttcttcaagg tttagctttt
     5761 ttattatata atctttattt acattatcaa tttttgaata atataatact tgttccacaa
     5821 aattttcaac tttatctagt tgagtatcta tctttttagt aatctcatta ctattatttt
     5881 ctataataag tttactagat gctataggcg tttttatttc atgaacccaa gtttcaatat
     5941 attctttata t
//
LOCUS       DAGSHT010000202         5942 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGSHT010000202 DAGSHT010000000
VERSION     DAGSHT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.208"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            378..4520
                     /locus_tag="MJB72_003159"
     CDS             378..4520
                     /locus_tag="MJB72_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBY8264227.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFTENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTTQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
     gene            4975..5322
                     /locus_tag="MJB72_003160"
     CDS             4975..5322
                     /locus_tag="MJB72_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY8264228.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(5472..>5942)
                     /locus_tag="MJB72_003161"
     CDS             complement(5472..>5942)
                     /locus_tag="MJB72_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8264229.1"
                     /translation="AFAGGEGDFTTAFEPTATAMEKEGSGYIVTSIGKDSGEIPFTAY
                     STTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKAMQPFFEDISLEDLTTVVDR
                     YKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKDIVNTEFANNAIKNIK"
BASE COUNT         2239 a          864 c          967 g         1872 t
ORIGIN      
        1 atttataata taaaagcata ctcttaatct tcaaaattta aaaagagtaa aaaataacaa
       61 aaaaatcact gacatttcac tgacaaattg caattttgtt attataaata cattgaaaat
      121 acttgattat tgaaaaatgg cataaaataa tacattttat gccatttgct attatttgat
      181 agtttttaac ttatgacata agatattttt taacatatac aataaaatat aaaaaaattg
      241 atattgcaaa taaaaaaaaa tacatattag atgttataga aaaaaagcgc acttataggc
      301 ttaaaatgtc acataaaaga gttttaaaga ataatatatt atgaacacaa attttgtaat
      361 cacaaaggag tgataatatg gcaacagtat caggacaaat agtttttgat agaaatagaa
      421 gtgctaccat tgattctgga gactcaggaa tagccaatat accagttgta ttacaagata
      481 tagcaactgg agttagactt gtggttctca cagatgcatc aggaaattat gtttttacta
      541 atgttccaaa tggtaactat agaattgtag aagcttttgg aactacagga ggagtaccaa
      601 caccaggaaa ttttgcaaca gcgatagtag gtccagttcc aattgcagaa atgccaccta
      661 ttagttctgt gacaagtcct cctattggtt caacaaactt agatggtaca acacctaaca
      721 cactattaat aacagtagca ggttcagata taacaaatca aaatatatta aatgggcctg
      781 tgatttatac acctatagag aatatattgg attcttgcgt atctgtatct aatataaacc
      841 ttattacaga tgcagataac ggaacattcg gtttttttcc accaggtaca ccaagtaata
      901 caggacctga aatagaacct tacccaaatg tgacgccaga ttttacgtat gttgtaccag
      961 acccaactaa gtttacacct ttagatggag aatatacagt acaaaatatt atgacaaatg
     1021 caatgagtaa tgttatagga gcttggtggc gtattgctga ccatactaca ggaaatgaaa
     1081 caggaagaat gatggtggta aatggtttta atcctggagc tgtatttttt aaatcaacag
     1141 tacaagttac acctaacact aattatttat ttagcacttg gattttaaac ctttttaagg
     1201 taacaggata tccaccacca cagctaggtg taagaatatt tgaccaaaat aataatatac
     1261 tatatcaagc aacattagga gctcaaattc cagttaatac aaacgcacca gaatggaaag
     1321 aagtaggtac tgtgattaat tctcaaaata ataatcaact tacagtagaa ttttttagtg
     1381 aaggagaaag cgccattgga aatgactatg ctatagatga tgtttcttta agagaaatag
     1441 ctgttccaac ttttactcca ataaaaacaa gtaacacaga aactgctaat gtaggtgata
     1501 taatcacata tacagttaca ttagataata cttgtgatag ccctttaact aatgtatttt
     1561 ttagggattt aattccaaat ggtttagagt ttatacctgg aactgtaacg gttgatggag
     1621 tatctgtacc aggagtagac cccaatacag gtttcccact tccagatatt ggaggaggaa
     1681 caggggttga agtaactttt gatgttgtgg ttgaaagtat tcctaaccca aatcctacta
     1741 ataatatagc tactatagat tactcctata cacctgtaga aggcggtata ccaaatgatt
     1801 tttcagtaga ttctaatcca gttcctgttg aagtaatctc agcagatatt gaagttacaa
     1861 aattatctga acctactata gtgaatccag gagaggaatt aatatacact ataaaagtag
     1921 taaataatgg accattccca tctgaaaatg tggttttaac agatgatgta ccagcttcta
     1981 ttgtaaatcc agagtattca ttagatggag gagtaacttt tcaaccatgg actggaagtt
     2041 taaatatagg gacattagaa gtaggagaga caagagttat tataattagg ggtattgtaa
     2101 atccatcaac agtaggaatt ataacaaata cagctgtagt atcttcaact acagcagacc
     2161 caaaccttaa taataataca tctacaatag agacagaagt gaatttatta gcagatattt
     2221 tagtaatgaa aacagcagaa cctaattcag ctgttccagg tactttattg agatatacta
     2281 ttcaagtaga aaacttagga ccagcaaatg ctgaaaatgt aatattaaat gatgatattc
     2341 cagcttctat tataaatcca gaatattcat tggatggagg agcaagtttt caaccatgga
     2401 atggaagtct taatatagga actttaaact ctggaatttc attaacagtt ttaattcaag
     2461 gaacagttag tttaaattct tcagaatata tagtaaatac agcaacagtt tcttcaacta
     2521 cacctgaccc agatttaagc aacaatatat ctaccattat tacaccagtt aacccacaag
     2581 caggtatttc tattataaaa gtagctgacg aggatgtagc tgttccagga gaagaatttg
     2641 tatatacaat tgaaattttt aatgaaggac catctaatgc tacgaatgta gttttaacag
     2701 atgatatacc agatgttatt ttaaatccag aatattcatt ggatggagga gcaactttcc
     2761 aaccatggac tggaagtctt aatataggta cagttgcacc agggcaatta ataagaatta
     2821 tcataaaggg attagtaagt tcaactgcta caggagatat tactaataca gcagaagtaa
     2881 gtgcagatgt accagaacca gttacggatt catcaacagt gacaactcct atagttccat
     2941 cagcagatat agaagtaata aaaactagca atatggatac agcagttcca ggagaaactt
     3001 tcagttatac tataacagtt acaaatttag gaccatctgc tgcacaaagt atcgttttga
     3061 cagatgatat accagatgtt attttaaatc cagagtattc attggatgga ggagtaactt
     3121 tccaaccatg gaatggaaat cttagtatag gcactttgga tgctggagaa ataagaagta
     3181 ttataataag aggaacagtg agccagacag cagtaggtac aattataaat acagcaacaa
     3241 taagttcacc tacacctgac ccaaatccag ataataatac ttcaacagat gaaacagata
     3301 taagtccatt agcagatatt tctgtgataa aaggcaacga accagttgct ataccagggg
     3361 gaagatttat ttatggaatt gaaattgcaa atgcaggacc atcttttaca gagaacgtaa
     3421 ctttaacaga taatatacca gcttctattt taaatccaga gtactcaata gataatggag
     3481 tgactttcca accatggaat ggaagcctta atataggtac tttagatgct ggagagataa
     3541 gaaatatcat aataagggga acagtaagtc aaacagcaat aggcacaatt ataaatacag
     3601 caacagtaag ttcaactaca cctgacccaa atctaaataa caatacttca acatcagaag
     3661 cagaggtaag ttcatcagca gatatttctg tagtaaaacg aagcaatcag attgttgttg
     3721 taccaggaga tgtactagac tatacaattg aggtgagaaa tgcaggaccg tctactactc
     3781 agaacgtaac tttaacagat aatataccag cttctatttt aagtccagaa tattcaatag
     3841 ataatggagt gacttttcaa ccatggaatg gaagtcttag tataggcact ttagatgctg
     3901 gagagataag aaatattata ataaggggaa tagtaagtca aacagcaata ggtacaatta
     3961 taaatacagc aacagtaagt tcaactacac ctgacccaaa tccagataat aatacttcaa
     4021 catcagaaat agatataagt tcatcagcag atatttctgt aataaaatta gcaaataaaa
     4081 cagaagcatg tgtaggagag caaatagatt ttgtaatagt agtatcaaat gcaggaccag
     4141 aaaatgctca aaatgttact ttaatagaca atgtttcaga taagcttaag aaagcagtat
     4201 tttcacttga tagaggtgta agtttccaac catggactgg aagtcttaat ataggtacac
     4261 taccagctgg aactttaaga gttattctat taagaggaat tataaaatca acatgtcttg
     4321 atagattaac aaatattgca gaaattactt caacaacacc agattcaaac cttaacaata
     4381 atatatcaag agtgcaagta gaaataaaac aatgttgttg taatgattgc tgttgtaaca
     4441 actgtagtaa ttgttgttgc aatgattgtg gttgtaaaga ttgttgctgt aattgttgta
     4501 aaaatgactt ttggtgttga ttttataaaa taattttgta taccttaaat taaactgaaa
     4561 taaaaaataa attcacatat gagtaaaata ttttataaat attaataaat tttctatctt
     4621 tgatatggac cacattcgta atactagtta ttaaatgaag tggtcctttt taattcatta
     4681 aaattattta ttctaattaa attttaaatg tattaaataa ttttaatttt ttataaaaaa
     4741 tattcatatg ttatttatat taaagtttag atatctaaaa atacttttag aatttattat
     4801 attatgttaa ttttttatat cttataagaa ttgtattttt ctaactataa aaatagtatt
     4861 atacttatat ttaaaaataa aagaatttta ttcctattgt gtaaagtgag aatactattt
     4921 tcttatattg gtataataat agtaaatcta atatagaaaa gaggtatttc tagtatgcat
     4981 gaaaataaaa agaatctatt agacggaagc ttagctcaat ataatgatac agcagttcca
     5041 aaaataccag tatttgctgg taacgatata acttcagaag tttattattt taaacctaat
     5101 caagttctta atgctcatag acaccctaat ggtgaacaaa tatttgtgtt cctaaaagga
     5161 gaaggtaaaa tgaaacttgg tgagcatgaa tgtgatgtaa aaaacggcga tactgtattt
     5221 gtaccaacag gagagtggca tgaaattact aatggaagca atgaagaaat ggtagccgtt
     5281 caaattacaa aaattaatgc tggagcagaa tacagaggat agatatataa gtacagttag
     5341 tactttaata tctattttat tatataaact ttaatctcaa atataaaaag agtcttttaa
     5401 aaggctcttt ttatattttt gttaattttc attataaaaa caagaaaata tctagctaaa
     5461 actaatatat tttatttaat attttttata gcattgtttg caaactcagt atttacgata
     5521 tctttataag gtgcttcctt atctagttct ttagcttctt tcataacagt catcaatttc
     5581 ttcaaacctt cctcttttaa tacaggattt tgagaccatg catctattga tttatatcta
     5641 tctacaactg ttgttaaatc ttctaaagat atatcttcaa agaaaggttg catagcctta
     5701 gctacttctt tgctagatgc ttgttgaatc cataattgac ctttataaac agcatttgta
     5761 aatctttgaa ttaaatcttt attgtttttc atgtatgatc tcgtagtaga gtatgctgta
     5821 aaaggtattt ctccagaatc ttttcctata gatgtgacta tatatccact accttctttt
     5881 tccattgctg ttgctgtagg ttcaaatgct gttgtaaagt caccctctcc acctgcaaaa
     5941 gc
//
LOCUS       DAGSHT010000203         5907 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGSHT010000203 DAGSHT010000000
VERSION     DAGSHT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.209"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1504)
                     /locus_tag="MJB72_003162"
     CDS             complement(<1..1504)
                     /locus_tag="MJB72_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264230.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDNKEKLEAVLEDSQIIIYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQV"
     gene            complement(1627..4170)
                     /locus_tag="MJB72_003163"
     CDS             complement(1627..4170)
                     /locus_tag="MJB72_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBY8264231.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(4448..4782)
                     /gene="rnpB"
                     /locus_tag="MJB72_003164"
     ncRNA           complement(4448..4782)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MJB72_003164"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4914..5591
                     /gene="srtB"
                     /locus_tag="MJB72_003165"
     CDS             4914..5591
                     /gene="srtB"
                     /locus_tag="MJB72_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBY8264232.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSNINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(5751..5825)
                     /locus_tag="MJB72_003166"
     tRNA            complement(5751..5825)
                     /locus_tag="MJB72_003166"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5791..5793),aa:Thr,seq:tgt)
BASE COUNT         1822 a          978 c          556 g         2551 t
ORIGIN      
        1 acacttgtac atcaagttta gtattatact tttcaaacaa atctatttct tgattttcta
       61 atattcttgg catatatgct gacaataaat ctaaattgtt tctttgttct tgtatttgct
      121 tataaagtga atttataact tctattctta tttcatcatt taactctttt tctaaaatct
      181 tatcaaattc agatttcata tagtaaatat ttccaaagaa attaaatatt atcatatcat
      241 tcatagcttt agaccaattc ttatatatca cttcaataga attttcttta agtagaggtt
      301 gctttgcata tataaataca tcttgcataa gtttttgaac catactttta cttaagttta
      361 aaagtacttt ttgattatta aattttattt ccgtttctac aaaattagaa ttttcgtttt
      421 caagttcttt attaattaca atgtctttta tatcactatt ttttatagat atgatttctt
      481 tttgaaaaga ctttagaata gtcaagctat cgaaatcttc ttgaataaaa aatggagcat
      541 gttttaatgt atatcccact atttcatttt ctatatcttt taggtcatct atttgcataa
      601 tttctctatt tttatcattt atatgtaggt taaatacatt tttattatca tataatatta
      661 tatcttctcc taaatttaac ttagaacaat tatctttcgc tgactttaca atagtcttct
      721 tagatttctt atttataaaa attaattggt cttgatattt tactaaaagc atgtttgtgt
      781 ttttataatc aaatccatct atatttcctg atatcttgca gaatactatt tcacaatctt
      841 taggaagatt tcctattttt tggttatcct taactctctc atttaaatca gatgggacta
      901 cactatctat attgttatta aatatagata cagaccttac tataatcccc tctctttcca
      961 tatgaaagta tcccttcaat gacactcttt tatcttctaa aatgtatctt tctatatcat
     1021 caaaatttat ttcaaatcta tttttcccac tgataaatat aaacttctca ttatctttat
     1081 taattccacc catataaaac ttattgtcat ctaaacttaa tatagtcata ttatccttta
     1141 gatagaatat aaattctctt tgacttttgc agattaaatc atattcatcc aaagttattt
     1201 tcatagtcaa atttgctgta acatttttac ttgaattttc actttttatg taaaaaggca
     1261 agttatttcc atagataatt atttgagaat cttctaaaac tgcctctaat ttttctttat
     1321 tgtctgaaaa actatatata tattttacac ctgtatcctt attaacttca tctgatttgc
     1381 tgattattat attccctaga tttgttatat gaagcttttt atattctttt gagtatctac
     1441 ttatgccact aagctcaaac ccatctgaaa gctcatatat gattttttct cctaatttca
     1501 acataccttc accacctaaa cagaattata agtattttat aaaggtgaac aaaatgtctc
     1561 acaataaata atattttttt taatactttt tattctgaga catagtttct ccttttctat
     1621 aatgctctaa gcaccttcaa ctatttcttc ataaagttta tctaaatgtt taacatattc
     1681 acgatatcca gatttgttct ttaactcact tcttaaatct tcatattttt gtttaagttc
     1741 ttcagcactc aaagaaggag ctgattcttt taattcttct aacttataat catagtactt
     1801 atcatacata gctatatatg tatcaataga tttcttagct agttcattac atttttcttg
     1861 tgcttctcca tattgagaat aatcacctat ttttatttta tagtatccat tttcttgaat
     1921 tattgttgaa gaaatacctt tagcctcata ttcttttgct gtttcttttg ctgttttttc
     1981 aactttaagt gaacctgcat aaacagtgta atatacacct ttaccatctt tatatgcttc
     2041 catttctttt gctttctttt cagcttgtgc tttttcttga gcctcttttt ctgcttttgc
     2101 cttttcttct gcttcttttt tctctttttc agcttgtgct tttttctctt cttcagttaa
     2161 tttattgtta gcttgttgtt gagttgatga ctttggaata ttctctttat catttttctt
     2221 attgttgcct actgttataa aatatcctac accaacaata accactataa ctactgctat
     2281 aatagtttct tttaatctac tcttcttaga aacaacctct tcatcatcta aagaatcttc
     2341 tttatctgat acataatttt tcatttcttt cttgttttct ctatatttgt ccttagaaga
     2401 atttttagat tttttattac tattttcttt ctttttaata ggtattacta ctgatttttc
     2461 attttctaat ttttcttctt ttatttcttt tgctttttta tcttctaagt ttttactttc
     2521 tttttcaagc ttcgattcat tatttgaaga tactttttca tctaagttat tatttattgg
     2581 tttttctatt tttacattac gttcttcttc tatatgttga cttggttctt cttgttttat
     2641 ttcttttaat tcattttgct ctatttcttc tgattttact tgatgatttt caactggttt
     2701 atctaaaatt atatcaggag aaatatcatt taaattctct tcttcttgtt caacttcttc
     2761 attgtctttt ttaataaatc caaataattt agccttcttt ttactctttt tattttctgg
     2821 ttcaattata tcttctttat tgctgtcgta ctctttatct gtagtatctt tatcttctac
     2881 aacgtcacct atatcttgat tattactatc tatttcttct ttaacccctt cttcagcatc
     2941 atcttctatt gaaatactac ttctaaagca aatatcttta tctgcatttt ttatcatgta
     3001 aatgtattct tcaatatcga tatctttatt tcttctagtt ctttttgatg caatttcatg
     3061 attttctaat tcactatagt tatcttttat tagattctgt gctttattcc atgtatcttc
     3121 actaatttct gttttatttt taattacata gtccacaaat tccttaaaat tttcaggacc
     3181 aaattctcca attatagtca gtctcattgc accttctata aggtctctat ttcttctctt
     3241 taaaaattct aatacaaccg ttgcacaagc aaaatcttta ataatttctt tgccaaaacc
     3301 atatccttca acagaatgaa gttcttctaa tccacataac ttcttatatt cattgtgtat
     3361 tgtttgagat acactttcat tatttaagct atttaactta tctataaact tcgattctat
     3421 atcatttgat tttgattcat gttttacttt tgaattttta atttcattta atagcatgtc
     3481 attttctgaa ttatctttac ctaattgcaa atcctcaatt tcagttgctt ttatttctac
     3541 tttgtcttgt tcgttattct cttccacatt attaggtacc acatctttat tgctagctaa
     3601 tattttgttt acacagaata taatttctct ttttaattct cttttcgcat ctgcttgatt
     3661 tttaagaaat aaatctccac attctttctt ttctaaactt atttttctaa gagatatatt
     3721 aagaagatct ttatataggt ttaaaacctt ataatccttt tctaaacgtc ttcttctctc
     3781 tgatagttcc attttatcat aagctaaaaa actatttaaa tcattggctt ttagagagtc
     3841 taatccctct ttcatagcct tatttacata ctttctacat tttgctgtag ttaattctaa
     3901 atctttttct aattcatcta tttcaggtat gtacttatct actatccatt tgccaataag
     3961 tggttctgta tttcttgcta atgtcttata aaacaatata ttaaatttta aaagctctgc
     4021 atattttgct tcattaaatt ctactttttc aaatttcttt tccaaaacac catctagctt
     4081 ttcatttatt ttagactctt gttttaaaaa acttgctgtg acatttaaaa cagtgtttct
     4141 tattatatta ttttcactca aaaccttcac tccttaaatc aattatttta ttgtaatatt
     4201 tacattgtgc aaagttgcta atttgattat gatatctaaa taattattta ctaaatttgc
     4261 tacatcttat atcatatcat attatttaca tcattttact ttttcttttt atatctatct
     4321 tatattatac catttttaat atctgtacta acttatatta caatattcct caaaatttta
     4381 aaagtagatt agacaaattt acaaataaaa aaacatcagt ttaaactgat gtttttgact
     4441 atcgaaataa ttaagcaagt ctataagcgg agttctgtat tagataatca tctttctagg
     4501 cctattgtca ccaataggct caagcgacct acctaccgga gagtacgagc agcactcttt
     4561 attcctatct tggtcttgct ccaggtgggg tttacatagc caactagtcg cctagctgct
     4621 ggtgagctct tacctcgcct ttccatcctt accacaatag tggcggttta tttctgttgc
     4681 actatcctta ggatcgctcc cactaggcgt tacctagcac cctgctctat ggagctccga
     4741 ctttcctcat gtaagattac atgcgattat ctggcttact taatatttat tttacttagt
     4801 tatgttaaca caaattcttt tacatgtcaa agtaatatta tactaaaaat ttaaatttat
     4861 tcaaaaatag tgtaatatag atatataaac tacatttaac tcgaggtgat atattgaaaa
     4921 aattatatag aatagtaatt aacattatac tagtattagt aatactttac agtggattta
     4981 atatatactc aaaacttacc aaatacaatc atgatactaa aatatcctca gaacttcaaa
     5041 aaaaagaata taaaaaagag gatttatcaa atattaacag tgattttaaa ttttggcttt
     5101 ctgttgagaa tacaaatata aattatccag ttgtacaatc taaagataat tcttactatc
     5161 ttgacaaaga tttttataaa aaagattcta tttctggtac actgtttatg gattatagaa
     5221 ataaatctat tgatgacaaa aatatcataa tatatggaca taatatgaaa aataaaacta
     5281 tgttcaacaa cctaaataag tttaaagatg ctgacttctt taaaaaaaat aataaaataa
     5341 aaataacctt gaatggaaaa gaatttttat atgatgtttt ttctgcttat attgtagaat
     5401 ctgattatga ttatctaaaa acaaatttta acaatgaatc tgattatcaa aattacataa
     5461 atgatattac ttctaaatca ttgtataaat ctcctattaa ggtaaatagc aatgataaaa
     5521 tagttacact ttctacatgt acctatgaat ttgatgatgc cagaatggtg attcatggta
     5581 gattgattta aaactcacag acacagttta aaacaataat tttaaactgt gtcatctttt
     5641 atacctataa tttgtaaatt agaccactaa aagtatttaa tacaaaataa caaatacatc
     5701 aaacaataaa aaacaataaa aaaagactat gctaaaaaca tagtctttac tggagctggt
     5761 gataggaatc gaacctacaa cctgctgatt acaagtcagc tgctctaccg ttgagccaca
     5821 ccagcacacc tattttattg taaattagca aaactaattt ttaaaagtta acactctaaa
     5881 aactacacat atatcttaat gaatgaa
//
LOCUS       DAGSHT010000204         5859 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGSHT010000204 DAGSHT010000000
VERSION     DAGSHT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.210"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(1..299)
                     /locus_tag="MJB72_003167"
                     /pseudo
     CDS             complement(1..299)
                     /locus_tag="MJB72_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1005..1898)
                     /locus_tag="MJB72_003168"
     CDS             complement(1005..1898)
                     /locus_tag="MJB72_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBY8264233.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(1978..2880)
                     /locus_tag="MJB72_003169"
     CDS             complement(1978..2880)
                     /locus_tag="MJB72_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBY8264234.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(2930..4090)
                     /locus_tag="MJB72_003170"
     CDS             complement(2930..4090)
                     /locus_tag="MJB72_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY8264235.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(4246..4551)
                     /locus_tag="MJB72_003171"
     CDS             complement(4246..4551)
                     /locus_tag="MJB72_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY8264236.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(4649..5623)
                     /locus_tag="MJB72_003172"
     CDS             complement(4649..5623)
                     /locus_tag="MJB72_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBY8264237.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         1850 a         1074 c          624 g         2311 t
ORIGIN      
        1 ttgttgtttt taaatcttga tttaaacttt caaaactctc aagtaataat cttgatgctt
       61 ttgaatcaaa tttatattgt tttcccttta attgttaatg tcaatgctat attttcataa
      121 gctattaaag tatataaaag attaaaattt tggaatataa atcctaattc attttgtcta
      181 aatttatcta agtttttgaa ttttaatctt gtaatatcat tattatctat tgctttttta
      241 atattatctt tgttttcata atacttttcc attttttcaa cacttaatac atttttcatg
      301 ccttatttct cctttaaatt tcttatatat ctattgtaat ttttaattta atttttttaa
      361 attgatttac atttcattaa tatttcactt ttgtagtaat tgtatattgc tacctataat
      421 tactaatata aaatgttcaa gaccaacaga ttttcttttg tgctttacta gaatcgtaaa
      481 atttacccaa ttctagaaaa acacaaaaaa tggaaggcaa tgccttccct ctaaaattta
      541 ttttattcta attgtatttt tctatatgtt ctttcaaaat cattggtatt aaatcattct
      601 tactatagtg ctttgtatca caaaattatt gccaatcttg atatgtaaat tcatttatct
      661 ttatactttt aagcttgaag tctaagtgtt tatcatttat tagattaatt attacatgat
      721 ttgtagtagt ttatatgtgg ctcttattgt taacttatac ttatctaaaa atgtttgtaa
      781 aactccaaga ttatttttaa tagataaagg ctattgcttt aatatttgtg ctaactttta
      841 aactaatctg ctatcttagt attatttaat ttttattaac ataattgtaa tttattgttg
      901 accacttttc aaagttgatt taacatatat aaaaagagtg tttaaaatga ttataaaaat
      961 cagtttaaac actctttttt tgctcatata tcttacttag taagttaaca tatatttaat
     1021 tcattcatca attttttaat ttcacatact tgtttttcat ttggttttac aaatggagca
     1081 gtcatataat cacttatttg taaccctctg atattcatag cttttttcat tgctggtata
     1141 aatggattgg ttattaagta aaaatccatt gctttattaa tatatttttg aatttctata
     1201 accttctcca tatctctatt attgtaagct tcagcccatt tggagcaaac ttctggaatt
     1261 aaatttgata atccccctat acaccctgct cccccagaca ttagattatg tataaagttt
     1321 tcatcataac cactataaac cttaaattct gggaactcta ttttcatagt atttattaaa
     1381 tctctggtat gtcccatcaa agttactgta tccttatatc caactatgtt tttatgtttt
     1441 cttgcaagtc ttaatgtaag ttctggactt aaatcataac cagttctatc tggataattg
     1501 tacaaatata tttcagcctc tgtattctta gctatttcac tataaaataa ctcaatacta
     1561 tcatcatcaa gagagaaata ataagggctt attatcataa caccatctgc tccactatta
     1621 tgtgcatagt ttgaaagttc tatagattca tttatacaca tacgacttgt acctataaac
     1681 acttttactc tcttatttac atgactagtt actaaatcta taagttcttt ttgcatatcc
     1741 atagacatat taaagaactc tccagtactt cccattatga ctagtccatc tacacctcca
     1801 tctataagat ggtcatacac ttttttatta ccttctttat ctaaatttcc ttcttcatca
     1861 aatattgtaa caactggtgt caaaaattct gctttcataa tttaaactcc taaaatttat
     1921 tttaatatgt tcattaatac atcagattta gctttaatat taattatatt aaaacagtta
     1981 cttaatgtaa ttttttaaaa ttccaatact ctcaatctca cattcaacta catctccact
     2041 actcatatat cgaggaggtg aaaatcccat ccctactcca gaaggtgttc cagtagctat
     2101 aatgtctcca gcctctaaag tcataccttt tgaaagttct gaaactatgt ctgaaacgtc
     2161 tgatatgaat aatcctgtat tagaactttg tctttcttca ccatttaccc tactacatac
     2221 tttaagtttt atgggaaaag gaactgcact tttatgtaaa atacatggac ctaaagaggt
     2281 aaatgtatct aatccttttc ctctaaacca ttgaacatgt tctttttgca actttctagc
     2341 agaaatatca tttataacag tgtaaccaaa gatataatct tctacctctt cttttctaat
     2401 attaacacct gttttaccta ttactactcc aagttcaact tcatagtcta gttgattatt
     2461 tatctcaaaa tgaccatcta tctcatcttc tggacctatt gcctttgtca cacgttttgt
     2521 aaaataaacc gttttttgtg gttcttcaaa actatcatca aagtgagttt gagtttcttc
     2581 aagatggtct ttatagttca ctcccacaca taagatatca tgtattggtt tttctatagg
     2641 aacatgtaca gtacatctct ctcttttaaa aacatcataa ttatagaatg tactttcatt
     2701 tataatagtt ttcaacttaa taatatcttc actagataca ttttgaatta attcatttaa
     2761 atccttaaaa tttctactga gatttaaaga gttaacatct ataataaata attcatcttt
     2821 agaaaaaact ccagttcttt tacattcatt ttctccttca gtaaaactta caaacttcat
     2881 aatctcaccc tttattaaaa ttctatgtat ttattttctt acttaaagat tatacatagt
     2941 tgcatactct agctattgcc atttcagtat atccaaggga ctctgctttt gattgttttc
     3001 catttgctac atctacaacc atatttaaca aatcatctgt taattcttcc attgtttgtg
     3061 gaccatagat aactggacta gcatcaaaat ccatattatc ttccatattt gcaaaagtta
     3121 ttttatttcc agttattttt attactggtg ctattggatt tcctgatggt gttcctcttc
     3181 ctgtactaaa taccactatt tgtgctcctc ctgctaccat accagcaaca gatgaagcat
     3241 catttccagg ggtatccatt ataacaagac ctttcttatc aatttgtttg gcataatcat
     3301 atacttcact tatttgtcta tgaccacctt tatgtataca accaagtgat ttttcttcta
     3361 aacttgttaa accaccagct atatttccag gagatggatt accttctcta acttcttctc
     3421 caaccaattt taatgaattt tcatatctat gtactatatg taatattttt tcttttactt
     3481 cttcatttac tgctctccta gctagtatat gctctgcacc tataaattca gtagtttcac
     3541 ttaaaattga tgttgctcct aaatcgacaa gtttatcact taattcacct attaatggat
     3601 ttgctgctaa tccacttgtt gggtcagacc caccacattc agttcctagt ataagttcag
     3661 atattggaaa ttcttctttt ctaagcaaag aagcttcttg tgccatttct ctagcataac
     3721 gaacagcttt ttctatagtt tttaaagttc catgttcttc ttgtattaca agagttttta
     3781 ttggcttatt tgttctctct tttattgcat caactaccaa atcattttgg caattttcgc
     3841 atcccaaaga tacaactatt acaccatata catttggatt tgctgcataa ccagccataa
     3901 catccatagt taattgttgg tctgaatgta cttgtgaaca tccattctga ttattaaagg
     3961 taactgcccc ttgaacttgt gatgctatta ttctagttgt atctgatgca cacacactcg
     4021 taggcaagat taatatgtgg tttcttatac caactcttcc atctggtctt ttatatccat
     4081 aaaatttcat actgatttcc tcctaaaata gtctatatac tttttataat ttttatatat
     4141 tataattttt attacactca ctaatcaaac agctaaaatc ctatttaaaa actatttgac
     4201 actaaaagca atgaatacaa cttttataac tctataaata ttattttata tattagtcat
     4261 aacttgttta tccaaatcct ctctaacact tctaacatta tgaatatgta catgttgacc
     4321 actttttatt tcctgatttg cttctcctat atgttcacca tatttagtaa ctttatcacc
     4381 tttggccata tcttttatag ccaatttatg ataaatagtt atatcatcta gtgctgtaat
     4441 cgtttttact gttttgtctt ttaatttaaa actaatttca ctacctttag ctattgcttc
     4501 aatagctact gcaacattat ctttttcatc aattatcact gcatttatca ttattcttgc
     4561 tctcctttat aaataatata attttattaa tatcctaaaa tctaattaaa gaatttatta
     4621 ttttaccaac aactcactgt aatataaatt aagctgttat tgtaccatcc tcattttgtt
     4681 ttgggaactt aggacatcca tacttcttag cccaccaact agttataaga ggtactaata
     4741 ttgcagtgac aactactgcg gctgccactt gtgttgtagc tgttgcaaca tatggttgcc
     4801 atgatgggtc aactaatgca acagcagcag gtacagcaat tgcatttcca gcagttgtag
     4861 ctacagccca tcctgcatat cctggacgtt ttcctataaa tttatcacag aatactataa
     4921 atatacctcc tacaaataca gatattaatc caagtaatac tcctgataaa cctcctttta
     4981 gtatacttgc aagatttatg ctactaccaa gtgcaaaccc tacaaaaggt acgagtaaat
     5041 tacaccctgg ctctaaaaac tctttcattt ttttgtcaaa atttcctatt atcataccaa
     5101 caagtattgg cactattgca gccaataatg acaacaatgg tacatttgca agacctgaag
     5161 tcccaagagc tattaatgta aaaaatggtc catcatttaa agtaagtaaa ctcatagttg
     5221 cacaatctgt ttcatctcca tatgaagtca tcaatgaaag atatatacta ccattactat
     5281 ttgtaactgc acttataaca gccaatgttg taagaccaag gacaccatcc attccaaata
     5341 gctttcctat aagtatacct atcaaagcac ctatagcaaa tttagaacca agaagtatac
     5401 cacctctctt taaaactttt ggcatttctc taaattgaag ctgagttccc aagcatacca
     5461 gttgaacccc aattatagtt gcagacccaa cacttgaaaa tactgctgtc gtaaacgaac
     5521 ctatttgtac aatatctggg caaaaggtat taatgaaagc tgctataaac attggcacta
     5581 tcatcatacc tgcaggaatt tttttaataa aatccattat cataaagtta atcctcctaa
     5641 aacatgatat ttaattactc ttattaagtt tttagcttta attctaacgg ccgtttcggt
     5701 tcgtatttaa gaactaagtt tttaattacg aatctttgaa ttaatgatag cattttccta
     5761 tttatgagtc aataaaaaaa ataaattttt gtcaaaattt tttcaaactt tctgataata
     5821 ccttgtatat tatcaaatga cttccttata tgcaaaaat
//
LOCUS       DAGSHT010000205         5736 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.211, whole genome shotgun sequence.
ACCESSION   DAGSHT010000205 DAGSHT010000000
VERSION     DAGSHT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.211"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(232..1062)
                     /locus_tag="MJB72_003173"
     CDS             complement(232..1062)
                     /locus_tag="MJB72_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8264238.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            1135..2862
                     /locus_tag="MJB72_003174"
     CDS             1135..2862
                     /locus_tag="MJB72_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264239.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMENMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGIRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            2899..4761
                     /locus_tag="MJB72_003175"
     CDS             2899..4761
                     /locus_tag="MJB72_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264240.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            5094..>5736
                     /locus_tag="MJB72_003176"
     CDS             5094..>5736
                     /locus_tag="MJB72_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBY8264241.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKT"
BASE COUNT         2179 a          657 c          962 g         1938 t
ORIGIN      
        1 aagtaaattt atattagttt aagtttactt acacctttat aaaaatacaa ataacattta
       61 cattcttaaa tgattaaagc tctttacctt ctaaacatgc ataacatatc gttataattc
      121 cagtaaataa gactaaaatg cccattacta agattacaat ttcatcatct attactccaa
      181 atgtttttaa agtaacttat ttcgctttgt atcataataa tttgtctcct tttacatttt
      241 aacttctgct gagatttgag ttagatattc gttagaatct cgcacactta tatcatatag
      301 aagatattct tcataaacaa aatccccaat acgtaaatta ttctgattaa aaaaatttaa
      361 taacttttca taagccttgt aaatagtttc ataactccct ttatgataag cagttacata
      421 aagtcccttt ggtttgattg atacaggata tttcttgtac tctttattaa cttttgtaaa
      481 taaatatgaa tatgattcaa agtctttatt aattatatct tcacctttaa tgattgagcc
      541 aatcgtacca taatcactca attcatagtt gtccataaag ttaatacatt catataaaaa
      601 attttcttcc tcatctccta aaaactgttc tttatatgta acaggtgttt tgaccaagta
      661 ctcttcttca tgttctttta aagtaatagt attcgcatct atattacatg cacttttagt
      721 aaatgaaata gtagattcca ttagagcttg aatattattt agtttttcaa tttcattttt
      781 aatatcaatg gttttctttt ctaaaagctc aattagtaaa ttaggtgttc ttttgtctat
      841 ataagcttta atttctttta aaggcatttt tacttcccta agtgaagaaa ttataccaaa
      901 aatgctaagt tgatggtatg agtagtatct atatccattt tctttcttta attctggtga
      961 gaaaagacct atatcatcat aatggaaaag tgttttttta ttgattccac aaatctttgc
     1021 aaattctcca gttgtaaaat aattttttga aacttgactc ataattataa ttcctccttg
     1081 actatcctgt tacagtatag tttatcatag tattttgtaa aggagtgtga ttatatgaaa
     1141 aaattaattc attttttaaa accatataga gttttaattg ttgtagtttt gatatttacg
     1201 tttttacaaa cacttggaac tttgtatata ccaacactta cagcaaatat agtaaacaat
     1261 ggtgtagtaa agggagatat agattatatt gtaaaaacag gtttaatgat gatgatagta
     1321 gctggtataa cagctttatc agctgtgtta gtttgtaaag tatcagcaaa tctttcatct
     1381 ggattttgta gagatattag agaagcagta tttataaaat cacaagattt atcaataaat
     1441 gattttaata atataggtac agcatcaatg attactagga gtactagtga tattacttta
     1501 ataggtcagt ctgtgtttat gtttattcaa ttagtattac cagcaccaat tataactgtt
     1561 tctggtctat tcttagcata ttctatagat aaagcaatga caattattat tgttgtagta
     1621 atgtttttat tcatgctttc agcattttta gttggtaaaa agcttataaa gttgtttaaa
     1681 atgatgcaaa taaagatgga aaatatgaat agggtgttac gtgaggtagt aactggagta
     1741 agagttataa gagcttttaa taggagtcgt tttgaaaaaa agagatttga tagaactgca
     1801 atagattatt ctgaaacagc aatttcaata aataaaatat ttgcagtact tatgcctatt
     1861 gtaatgttaa taatgaattt aggtatagta agtattattt ggtttggtgg gattagagtc
     1921 agtaatggaa atatggaaat aggtcatata atggcacttg ttgaatattg tatcttaatt
     1981 ttattctatt taatcatggg agtaatggta tttatgtaca tacctagagc aggtgcatgt
     2041 gcagatagag taaatcagat attagatata gaacctgaaa ttgtagatgg aaatggtcat
     2101 aaagatactg tctcagagag aggacattta gtatttaaaa atgtaacctt ttcatatgca
     2161 caatcagaag aacctgtgtt aaataatata acatttgaag caaaatctgg agaagtaact
     2221 gcaattatag gaagcactgg ctcaggcaaa tctactattg caaatataat accacgtttt
     2281 tttgaaattc aatctggaga aatatctata aatggtcagg atattaagaa gattcctcaa
     2341 aaagaactta gagataagat tggatttgta ccacaaaaag cttttttatt tagtggaaca
     2401 atagaagaaa atataaggta tggaaaagaa gatgcaagta ttgaagaagt aaagcatgct
     2461 gcaagtatag ctcaagctga tgaatttatt tctgatatgg aagacaagta tgactcattt
     2521 gtagcacaag gtggaaataa tttgtcaggt ggtcaaaaac agagaatatc cattgcaaga
     2581 gcgttagtta gaaagcctga agtatatgtc tttgatgata gtttttctgc attagatttt
     2641 aaaacagata aaagacttag aaaagcgttg aaaaatgaaa tcaaagattc atcagctata
     2701 attatagcac aacgtataag tacaatcatg gatgctaatc agattatagt tttaaatgat
     2761 ggaaaaattg taggtattgg aaaacataaa gatttacttg aaaattgtga agtttataag
     2821 caaattgcgg attcacaact tagtaaagaa gaacttgcat aagatgaaga attaatctga
     2881 tatttggagg tgtatgatat gagtgaaaaa agaatagatg aacaagatat agaagtactt
     2941 agttttgaag atggcatgtc agcagaaaaa gctaaggatg caaaaaaaac tactaagaga
     3001 ttattaaaat atatggcaaa acaaaagttt aaaatattaa ttatttttat ttcagtattg
     3061 ataagttcag cgcttacagt tttagcacct atggttatgg gaaaagctat tgaccaacta
     3121 tttaatggaa taaaaactgc agtccaaaca ggtacaaaat tcagtgtgaa ttttagtact
     3181 atggggggta tagtatcaat acttctaggg ttgtacttaa ttagtgcagt atttatatat
     3241 attcaacagt atattatgtc aggagttgca caaaatctag ttcttagcat gagaaaagat
     3301 ttaagtgata aactcaataa acttcctctt aagtactatg attctcataa aaagggggaa
     3361 acacttagta tagtaacaaa tgatttagaa aaagtagcag atagtttaca agaaggatta
     3421 atgcaactta ttacagcagt agttacagta gttggttcta ttgtaatgat gataagtata
     3481 agtgtaccac ttacaatagt atcagctata acattacttg tttcattggg tattactgtt
     3541 gtaattgcaa gaaaatcaca agaaagattc tctgaaaatc aaaaggcact aggagagtta
     3601 aatagcaata tagaagagat atttactggt caaatagtgg ttaaagcatt tagtaaagaa
     3661 aaggatacta taagaaactt taaaatatta aatcaaaatc tttataatgc aagtagaaaa
     3721 gcacagtttt cgtcatatgc aatatctcca attattagat ttataaacct aattggctat
     3781 gttattatag cagttgtagg tgggatattt gcatcaactg gagcaatgac tcttggtagt
     3841 atacaagcgt ttattcaata tgtaaatcaa gcatcagagc ctactacaga aatatcttat
     3901 attgtaaata tgcttcaagc agcaatagca tctgcagaga gagtttttac agttatggac
     3961 gaggtggaag agataaagga tgaagaatct tcaaaggtta ttagcatgcc taaaggtaaa
     4021 gtacaatttg agcatgttaa atttggatat agtgatgatt ttattcttat gaaagatatt
     4081 aatatcaatc taaatgcagg agataagatt gcaattgttg gacctacagg agctggaaag
     4141 acaactcttg taaatttact tatgagattt tatgagatac aaggtggaag gattactatt
     4201 gatggtgtta atataaaaga tttaaagcgt ggagaactta gaactatgtt tggtatggtt
     4261 cttcaagata catggctgtt taatggaagt ataaaagaaa atattgcata tagtaagtca
     4321 gatgccacaa tggatgaaat tgtaagagca gcaaaatcag caagagtaga ccattttata
     4381 aggactttac cacaagggta tgacactata ttgactgagg atgcttctaa tctatctcaa
     4441 ggtcaaaagc aacttctaac aattgccagg gcaatacttt cagacccatc agtattgatt
     4501 cttgatgaag caacatctag tgtagatact agaacagagg tagaaattca aaaggcaatg
     4561 aataacttga tgaagggaag aactagtttt gttattgcac atagattatc aactattcga
     4621 gatgctgatt taatattagt aatgaaagaa ggaactatta ttgaacaagg aagccataag
     4681 gaacttattg aaaagaaagg attctatgaa gaactttata acagtcaatt tacaagtgaa
     4741 tatgatgaag atgttgtatg attatcaagt tttaggcaac taaatgattg ataaatatta
     4801 agttaaatat agtaacatgt aaggttatat ggaataagag tatttctcta tttcatataa
     4861 ccttttttat ttatcaaaat aagataaaat ttcctttcta aattagattg aagaatgtat
     4921 ttatcaatat aaaagtaaaa taatcattat tattagtgaa gtaaatattg tttgccacat
     4981 atatgccata aagatgcact tattttacgg agatttaaca aagtagtgta aagtatactc
     5041 taaatataaa ataaatcaaa tagagttaaa taaattgaga ggtgatataa atagtgaata
     5101 aaaaattgaa ctatagattt gaaatgaagc acaaaataac tgaagcagat gtgttagctt
     5161 taaaatcaag actctatcca ataatgaaga aagatgaaaa tgcatctaaa gatggaaaat
     5221 atcttattag gagtttatat tttgatactc ctgaagataa agcacttttg gataaattaa
     5281 atggcgttgc aataagagaa aagtttagga taagatttta taataatgac tgctcttata
     5341 taaggttaga gaaaaaaatc aaacattaca acatgacatc aaagctatca actaatttaa
     5401 ccaaaaaaga agtaataaat atactaaata atgatattga atttctaaaa gagtcgacaa
     5461 attgtttatt aagagaattt tatttgaaat taaaatgtga aagattagag gcaaaaagca
     5521 tagttgatta caatagagaa gcttttattt atccagttgg aaatgtaaga gttacaatcg
     5581 atagtgatat taaaacttct gtaaattcgg tagatttatt taataaagat ttgccaactg
     5641 tttcggtaat tgatgagaat atgactgtat tggaggttaa atatgatgaa tttataccag
     5701 attttataaa ggatttaatt caaattaata agacta
//
LOCUS       DAGSHT010000206         5660 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.212, whole genome shotgun sequence.
ACCESSION   DAGSHT010000206 DAGSHT010000000
VERSION     DAGSHT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.212"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            146..550
                     /locus_tag="MJB72_003177"
     CDS             146..550
                     /locus_tag="MJB72_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBY8264242.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKGDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            1196..2056
                     /locus_tag="MJB72_003178"
     CDS             1196..2056
                     /locus_tag="MJB72_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8264243.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            2046..3917
                     /locus_tag="MJB72_003179"
     CDS             2046..3917
                     /locus_tag="MJB72_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY8264244.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3937..5391
                     /locus_tag="MJB72_003180"
     CDS             3937..5391
                     /locus_tag="MJB72_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY8264245.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         2210 a          576 c         1005 g         1869 t
ORIGIN      
        1 actgacagtg gggtctttta acctaccagt tggggcatat atattgatgc taaatatgag
       61 ggatgttata ttttaataaa aaagataaca tccttttttt agtgcattat atcgagtttt
      121 aaataataaa tgaaggagga ttaatatgaa aaaatataaa gttattttta tagtattaat
      181 tataggtatt ataagtgtaa cagggtttgt tgtatataaa aactgcatta aaccatggaa
      241 agatgctgaa acatatattg ataagtatat ggtaaaacaa gatatttcaa aagataatat
      301 aaaaagtatt acaaaagaaa aagccaagaa ggcaagttac gaaggaatac tatacaaagt
      361 gtattacaaa ggtgaccctc gatatgagta tcaatatttt tatagtgata attattatgc
      421 tctatatgta aataaagtaa tgttacaaat ttatgatatg gaaaataata ataaactttt
      481 aagaagtaat gaggaattaa aaagtgtaaa gtatccacca atatatctta aatataagcc
      541 agataaatga tttatgacaa aatagaatga tttttattaa aatttattta aaaatatcac
      601 tagagtgttt aaataaaact aaatttaagt atttatatat tagtatatct tactagtgtt
      661 taaataaaac taaatttaag tatttatata ttagtatatc ttactagcat ttaaatgaaa
      721 ctaaatttga agctttacct attaatatat cttaatagtg attaaataaa actaaactta
      781 agactttata tgttaatata tcttaagaat ttatttgtta acatacagtt cattatattt
      841 aaaagcatta ctatgataat tcataatagt aaaatagata atggttcaga caatattata
      901 aatattagtg acggtaaaat agtatcaatt tagtatataa accttgtcag ataatcgcat
      961 aacagagctt gtagttatag aataaaaaaa tatctaggaa accgttgaca gatttatatg
     1021 gatagtataa catatagaat atgaaattaa acaaaaaaga attttaatta tggcgacgta
     1081 gtcataattt tgagtatgtt actgattagc aggcagaact caagtatata tgtgaaaaat
     1141 agagggatac tctatacata tatgcttgag ttctttttat tgaagggagg aatatatgaa
     1201 aataattcag attttaaata ataatgtagc cttagtaaaa agaggtaaaa atgaaattat
     1261 tgtatttgtt aaaggaattg gctttaaaaa aagagtggga caagtaataa atgaaaatga
     1321 aatagagaga acatatatac tagattcata cgatatgcta gaacacttta gttatctact
     1381 acaaaactca gactccaata atatagttct tattaatgaa ataattgaat atggtgagaa
     1441 agctttaaat ataaagtcaa gtgattatct aagcttaacc ttactagacc atttagagtt
     1501 tttacttaaa agatgtgaaa agaatcaatt tataaaaagt cctttggtat ggaatgttaa
     1561 aagattttat ccaaagcatt ttgaaattgg aatgcatgca ctaaaattaa ttggtgatag
     1621 taagggaatt aatcttcctg atgatgaggc agtatcaata gcacttcatt ttgtaaatat
     1681 ggaggtaaat aaagagtctc atgatagtac aattgtagag ttgagaactt tagcagatat
     1741 agtttctata ataaaatatc attttaatgt agagctagat gaaacttcaa caaattatat
     1801 gagatttact actcatctac agtactttat ccaaagaatt atgtcggatg aagcttcaat
     1861 agatgaagat aattctatag atttatataa acaagtaagt caactttatc caaagtcatt
     1921 tggaagtgtt caaaagataa gaaaatatat aaaatcacaa tttaatgtcg atatatctat
     1981 aagtgaagaa acttatttaa tgttgcatat aaatagggtg actcaaagac tggaggcaaa
     2041 atcagatgaa atataaagat ttaaatgaaa atataataaa acttataggg ggaaaagaaa
     2101 atataaagtc ggtagcgcat tgtgttactc gtttaagatt tactttaaat gataggagta
     2161 tcgctcaaac agaagaaatt aaaaaattaa aaggagtaat tgatgtagtt tctaatgatg
     2221 ttgcttatca aattataata ggtactcatg ttgtagatgt atataacgag tttatgtcta
     2281 tgataggttt aagttcaagt gaatcaagta taaaaagtga aagaaaaaag ggaattagag
     2341 gtatcaaagg tatatttaat tccatctttg tggtagtctc agagactatg acttcagtaa
     2401 ttgaagtact acttgcagct ggtatacttg caagtatatt agcactttta aatatgtctg
     2461 gcatacttca gagtgacagt cctacatatc aaatattaga tacattaaga agtgctacat
     2521 ttcacttctt accagtatta attgcaatag catcagcaaa aagacttaat gtaaatccct
     2581 atatagcagt agtgttagca gttacgttat tatcttcatc aatagatggt gtagaaggat
     2641 taagtttatt tggtataaat ttacaggcta caacttatgc aaacagtttt attccaatac
     2701 ttttaggagt atgggcaatg ggaattataa ttgaaggttt gaaaaaaata ttacctaaag
     2761 cactacacta ttttttagtt ccagtattat ctttaataat aactctatca gttacattaa
     2821 ctgtatttgg acctattggt atgtggattg gtgatggatt aaactatgca tgtatgtttt
     2881 taattgatac attgggtaat tggagtgttg ttgcactgta tgcagcttta caaccactct
     2941 taatagtttt tggtgcagca aactttacta tgcctatatc tttaaattct gtgacaacat
     3001 tggggtatga cccaattttt atgggcgctg caacaatatc tgatattgca gtttgtggtg
     3061 caatgtttgg atatttctta aaaactagga ataaagaaca aaaacaacta tttggtacag
     3121 taagttttag tgcacttatg ggagttacag aaccttcagt gtttggtgtg tttatgaaat
     3181 atagaagacc atttgtagca gtaatgatag gtggaggtat aggtggttta atagcaggtt
     3241 tagctcaagt taaaacatat ggaatggttt ggggattagc tgcattacca acatatcttg
     3301 ttggaggaga agtatctaac tttacattta tgataataag tgtagtagta tcatttgtag
     3361 cagcaacagc agcttcctat ttactaggaa taccttcaga tgaaaaagaa gaagttaagg
     3421 gtgataatga aaaagaatta gaactaataa tcaacgagaa caattcaaga actattagta
     3481 ttggaaatgt tgcaaaaggt caggtaattg cacttgggga agttaatgac aaagcatttt
     3541 catctggagc attaggtaaa ggggttggag ttatacctag tgaatcaaaa tcaactgtta
     3601 tatctcctgt agatggaact ataactatag tatttccaac taaacatgca tatggtatag
     3661 tgactgatga aggattagaa atattaattc atattgggat agatactgta aacttagatg
     3721 gaaaattctt tgaaagtctt gtaacacaaa atcaaagggt taaaaaagga gatgctttag
     3781 cagtatttga atctgataaa attatagagg aaggctttga ttctactata ataactgtag
     3841 ttacaaatac tagcgattat ttagatgtaa tctcatcaaa tgatgaatca gaagaattat
     3901 taactgtaat agtataaatt ttaggaggga aaattcatga atattaaaga tacatttttc
     3961 tggggaggaa gtattgcagc ccatcaatgt gaaggttcat gggatagtga taacaaagga
     4021 cctgcaatta tggattttgt aactaagggc tcttatgaaa cgccaagagt aattactgat
     4081 aaaatagaag aaaaattgga ttatccatct cataatggaa tagattttta taatagatat
     4141 aaagaagata tagctttatt taaagaaatg ggatttagtg cattaagaat atctatagat
     4201 tggtcaagaa tttttccaaa tggagatgat gaaaatccta atgaattagg gattaaatac
     4261 tatgaaggtc tgatagatac attaatagaa aataatatag aacctattgt aacactatat
     4321 cattttgaat tacctatgaa tttagtacat aaatatggtt catggaataa cagaaaactt
     4381 atagatttgt atttaaagta cagtgagact gtaataagaa gatttgatga taaggtaaag
     4441 tactgggtta catttaatga gatgaaccat attgaccctc aaacagagca ctcggatatt
     4501 tttacttata tgatagcagg tattaagtat agtgaaatag aaaataaggt tcaaacattg
     4561 gcaaatgtag ggtataatat gactttggcg ggtgtaaagg ttgcaaagtt ggctagagaa
     4621 atcaatccca acaataaagt tggatgtgta tttggactaa atccaattta tccatacaat
     4681 tgtaatccta gtaatgtgtt aaatgcattt ttagataatg accgtgatta ttatcaaatt
     4741 gatgcaatgt gcaatgggaa gtttccacaa tataaattaa aagaatatga aaaaaataat
     4801 ataactattg aagtgactga tgaagacaaa aaagcattta aagaaggtaa gttagatttt
     4861 ataggactta attattattt ttcaagtgta tctactccaa aagaaaattt atcaggtgat
     4921 aaatctttat ttggtgggat tcaaaatcca tatttggaac aaagtaagtg ggggtgggca
     4981 atagacccta taggtttaag atttaccttg aactatattt acagaaagta tcaattacct
     5041 atcttaatta cagaaaatgg tttaggggca tatgataaaa tcgaagaaga tggaactata
     5101 aatgatgatt atagaattga atatttaagt aagcatctat ctcaaatgaa gtctgcaatt
     5161 gaagaggata atgttgaatg ttttggatat ttgatgtggg gaccaattga tttagtcagt
     5221 gcaactacag gtcagatgag taagagatat ggatttatct atgttgactt agatgatgaa
     5281 ggcaaaggaa gttttaagag atataagaaa aaatcatttg attggtacaa agaagtaata
     5341 aaatctaatg gagaagtttt agatttttct acggatatag tagtagggta ggaataagat
     5401 aaatagaagt taggaactaa tgttaattag tgtctagtaa agcatttaaa atagagtata
     5461 gggtctttaa agtaaagtgt gtattcttat taaaagtaat ggcaactaaa attactataa
     5521 aaaggagaat acacattttt tatgtttata cttaagtaaa tatttagaca tattcatagt
     5581 ataatttact cattatataa aaatagtatg atttactcac tatataactt tataaatttt
     5641 ctggaaaaat gtcagaatat
//
LOCUS       DAGSHT010000207         5548 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGSHT010000207 DAGSHT010000000
VERSION     DAGSHT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.213"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            146..1846
                     /locus_tag="MJB72_003181"
     CDS             146..1846
                     /locus_tag="MJB72_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264246.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2016..2633
                     /locus_tag="MJB72_003182"
     CDS             2016..2633
                     /locus_tag="MJB72_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264247.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2994..4187
                     /locus_tag="MJB72_003183"
     CDS             2994..4187
                     /locus_tag="MJB72_003183"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBY8264248.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4205..5497
                     /gene="lysA"
                     /locus_tag="MJB72_003184"
     CDS             4205..5497
                     /gene="lysA"
                     /locus_tag="MJB72_003184"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBY8264249.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
BASE COUNT         2201 a          575 c         1054 g         1718 t
ORIGIN      
        1 acacgtataa tgttaaatat agttgaataa gcagaactat aatatataaa gatatcttaa
       61 tatataataa atatgataaa tatttatttt ttgtaaatat atggatataa tagtaataat
      121 aacatttttt agagaggagt attaaatgaa acgatatctt aaaacatatg gagttgcatt
      181 aataattgta ataatttatg catttattaa attacctgta ctaagattag attttacatc
      241 tttattttca gtaggaataa ttttctttgt tgtagcagga atactagata tgatgttaga
      301 tagaaatgaa agagcatcta aaatggctaa atataatttt tcaatagcaa tagctttgct
      361 tgtgtatatt gtagtagttc cctttataac ttctacacct gtacttcatg caaaatcata
      421 tagagaacta ttgggaaaag taactgaaag taaatttact gatgacatta gccctgtcag
      481 tgtaaatgac attcgtcttg tagatgaaga tatggcaatg aaattaggtg acaaaaagct
      541 tggagaagtt cctgcaatag ggagtgtatc caaattagga aaatttcata tacaaaatgt
      601 agatggagag ttgtactggg tagcgcctct tgttcacaga gacataatta aatggataac
      661 atctctaagt ggtacaagtg gttatgtaaa agtatcagct agtaatcctc aagacgtcca
      721 attagttcaa gaaatagatg gaaagccaat aaagatagtt taccaaccag aagcctactt
      781 acatcaggat ttacaaagac atttatacat acatggtatt gtaaatgttg gtatgacaga
      841 tttcactctt gaaataaatg atgaaggaag accttattgg gttgtaagtt tatatgaaca
      901 taaaattggc tatggaggag caaatgcaac aggaatagcc actgttgatg cagaaactgg
      961 aaaaataaat gtttatgatg ttaaaaacac gcctaaatgg gtggatagaa tacaaccaca
     1021 aagctttgta acagaccaaa ttaaagactg gggtgtttat gttaatggat ttttaaactc
     1081 tgttatatca gaaaagggag ttttagtacc aacagaaggt acatcattag tttatggcaa
     1141 tgataacagg tcttattggt atacaggtat aacatcttca ggtggagatg aatctacaat
     1201 tggatttatg ttagttgatt ctagaacaaa agaagctaga ttatataaac aaccaggagc
     1261 tactgaaacg gctgcaatga aatcagctga aggtaatgtt caagaaaaaa attatgaagc
     1321 gacgttccct gtaatgtata atatattggg tcagccaaca tatgtatcat cattaaaaga
     1381 taaggcgggt cttgttaaga gagttgcatt tgtatctgtt gaagattata atgtacttgg
     1441 aataggtgaa gataagaatg aagcattaag aaactataag gatgcattag aatctaaggg
     1501 taatgattta aaacttgata atgatttaaa agatgaggta ttagaaggca cagtgacaag
     1561 aataagccca gatgtgcgag gtggtaacac taattattat gtaactttag attcaaataa
     1621 agatataata tttagggcta cttcaaaagt atctagcgaa ttgccattga ctcaggttgg
     1681 tgataaagtc aaaatatcgt attcaaaaga agaatctggt gtaatagaaa tgtcagaatt
     1741 tgataaccta aatattgcaa actttgaaga taaaaaagca aaagaaaaaa caggaactga
     1801 tgaaaataca gattccaaag attctgaaaa tcaaggtaat gtataattta aataaaaaga
     1861 ttgataatag tatttaacat agtgatagct aatttttata gaatttaatt ctataaaaat
     1921 tagctatttt attttaattc tttgtaaatt taatgtcata aaattaatat aatattagta
     1981 caactttgca aataatactt caaggagaat gtactatgac gaatatggaa aaagagatat
     2041 tacttttaat tagcagggtt agatgtgtta cagagtctca atttagaaaa atttatggag
     2101 accaaaaaag atacaataaa aaaaatttta aaaaaacttt gagaaaaatg tgtagtgaat
     2161 atacactaaa aaaatatcct tgtgatgtgg attattataa ttataaagac ctctcttatg
     2221 tatactattt aaacggtagt aaacttttta agggagatga tttaataaag gctctagtag
     2281 gatcagaatt agttgttaga atcaatactg caggttgtga tgtaaaaaga ttttaccgaa
     2341 atgttagtgt agatgatgtg aaatacgatt tatttattga atatacagac caatttggta
     2401 atataaagca agttttagtt gatgttgact taaataatga aatagacatt tctaaatacg
     2461 aaaatctttc taaaaaaata gataaatcta caattccatt ttttaaagta ccaaacgttt
     2521 tggtagttac accagataat ctatccgaat taaatgattt agagagatat gataatgagt
     2581 atagcataaa ttttatagat ttaggattaa ataaactatt taattgtata taagctaact
     2641 ttagaaaaga aaactatcga tttgccacat tttgtaaaat aattgttgca atattatata
     2701 gaatatgata aaactatatt aataaattaa ttaaaaataa tgagcctagg gtagaggtag
     2761 ctgtattcaa tagtattcaa taggagctgg taagcgttga taattgatga aagggtatat
     2821 agccgaagaa attttgttga cggacaaatt ttctggactt atgcagaata tgcataaggc
     2881 tgtcactttt aaagtggtga gctacaaggt tacttggtct atgaaattta cacgtccaaa
     2941 gtataccttt gggcgttttt atttatcaaa atttagcttg agggggagaa agcatgagta
     3001 taattgttca aaaatatggt ggaagttcag tagcagatac tgaaaaaata aaatcaatag
     3061 cagaaaatat aatagaacga agaaaagaaa atccacaaat ggtaatagtt gtgtctgcca
     3121 tgggaaaatc cactgatgaa tatataactt tagcaaagga attgagtaat gaacctagta
     3181 agagggaatt agatgcttta atgtctactg gtgaaatgat ttcagcatct ttattatcaa
     3241 tagcattaaa tgcacttgga tgtaaagcta taagttataa tgcttaccag ttaaatataa
     3301 aaacgagtgg tcttcatgga aagtctcaaa ttgatgatat aaacgtaaac agaattaaaa
     3361 atagtttaga tgaaggaaat gtcgtgatag taactggatt tcagggaatc aacgaagatg
     3421 gtgatgtaac cactcttgga agaggtggtt cagatacatc tgctgtagct cttgcggtta
     3481 aattaaatgg aaaatgtgag atatatacag atgttgatgg aatttatttt acagacccaa
     3541 gaaaatattc aaaggctagt aaacttgatg agattgaata tgaagaaatg cttgagttag
     3601 cgagtttagg agcacaagta atgcattcaa gaagtataga gcttgcacaa aaatatggtg
     3661 ttgaaatcta tgtcggacgt acatgtggta cagaaaaagg aacatatata agagggggaa
     3721 aagacatgaa attagaggat aaagtaataa caggattagc tactagtgat gatgacagtt
     3781 ctataacaat aaaagatttt aaagctgaaa atatatctag cttatttgaa gatatagcaa
     3841 ctataggtat aagtgtagat atgataagcc aaactgcacc tattttagat aagataagtg
     3901 tatcatttac agttccaaaa gaagagttgg gagaatgtaa aaagatagta tctaaatata
     3961 cagatgaaga acatgtagtg attgataata atatcactaa attttcttta gtaggtcttg
     4021 gtatgaaaaa tacttctgga gttgctgcta aagtatttaa gatattcaat gaaaatggta
     4081 taatgataaa acttataact acatcagaga ttcgtataac ttgtgctata aattctgatg
     4141 ataagcaagt agctatagaa aaaatagcag aagtatttaa tatataaaat ttggggaggc
     4201 tattatgaaa cttcatggaa ctatgactgt aaataatgat gaattatata ttggaggagt
     4261 gagttgttta gaacttagta aaaaatatca aactccactt tatgtatttg atgaagcttt
     4321 agttagacaa aattgtagag aatatataga atattttaag gctagagagg gaaaaaataa
     4381 ggttgcttat gcagggaaag cttttttacc tctttacatg tgtaatctta taaatgaaga
     4441 acaaatgtgt ttagatgtag tttcaggagg cgagttatat acagcttata aatcaaattt
     4501 tcctatggaa aaagttttct ttcatggaaa caataagact aaagaagaaa taaacatggg
     4561 attagattta ggtgtaggta aatttgtagt ggataatttt tatgaattag atttgcttga
     4621 agcattatgt gaagataaag gtaaaactca agagatatac tttagaataa ctcctggtat
     4681 agaagctcat acacatgatt atataaaaac tggacagata gattctaaat ttggatttgc
     4741 tttagtaaat ggagatttat ttagagcaat agaaaaatta agtgaatata aaaatattaa
     4801 acttgtaggt cttcacgctc atataggttc acaaatattt gatgtagaac catacataga
     4861 tacagtggat ataatgatga acttggtaaa agaaataaaa gaaaaattca acatacaact
     4921 aacagaaatt gacctaggtg gaggagttgg cgtttattat agcgaaggag ataaacctaa
     4981 aactataaaa gagttttgtg aaaccataat cactaaagca gaaacttcat gtaaagagct
     5041 tggaatagaa gtacctacat tggtaataga accaggaaga tcaatagttg caaatgcagg
     5101 aagtacattg tatacagtag gttctataaa agatataaaa gatgttaggg tttatgtaag
     5161 tgtagatgga ggtatgacag ataatgtaag accttcgttg taccaagcta gttatgagtg
     5221 tagtattgtg aataaaatta atcatgaggc tatgaatcat gtaacaatag ctggaaaatg
     5281 ttgtgagagt ggagatatat taattggaga tataaacata atggatatta aaagtggaga
     5341 tatacttata acaacatcaa ctggtgcata tggatattca atgtcttcaa attataataa
     5401 aataccaaga gcagcagtag tatcagtatt tgatggaaat gataagataa tttgtaagag
     5461 acaaagctat gaggacttat tagctttaga gatataaaat agagaagtat ataataaaaa
     5521 ttaaatatta aagtttatta tgagcaga
//
LOCUS       DAGSHT010000208         5538 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGSHT010000208 DAGSHT010000000
VERSION     DAGSHT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.214"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            138..758
                     /locus_tag="MJB72_003185"
     CDS             138..758
                     /locus_tag="MJB72_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBY8264250.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(924..1460)
                     /locus_tag="MJB72_003186"
     CDS             complement(924..1460)
                     /locus_tag="MJB72_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBY8264251.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1573..2247)
                     /locus_tag="MJB72_003187"
     CDS             complement(1573..2247)
                     /locus_tag="MJB72_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBY8264252.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLIATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2450..4324
                     /gene="asnB"
                     /locus_tag="MJB72_003188"
     CDS             2450..4324
                     /gene="asnB"
                     /locus_tag="MJB72_003188"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBY8264253.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4416..>5538)
                     /locus_tag="MJB72_003189"
     CDS             complement(4416..>5538)
                     /locus_tag="MJB72_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBY8264254.1"
                     /translation="WDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSII
                     KNFAVKLLCWIDYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYI
                     NPNKDILDLYPECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPN
                     SKNTLNNTNLTSKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINN
                     EGYILTNLHVVNDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKV
                     SKKPVRGQKIVAIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDM
                     FGNLLGLISAGYDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         2134 a          651 c          823 g         1930 t
ORIGIN      
        1 ataaaataat tataccttaa ctgtaaactt atatagatta tttacttttt aatattagat
       61 tcattttttg agattggtat ataataaaca tatagagtat ataataatag ttaaagttaa
      121 aaaaggggaa aagcaatatg cagtatatac aagaactact tgatttgact caaaattatt
      181 gggtgttagc cacaataatc ggcttattaa gtgcatttat agaaagcttt ataccagcct
      241 taccacttgt tgctatagtt acagcaaatg ctgcaataca aggattattt attggatgtt
      301 tgttatcatg gattggctca ggacttggga caacttcatt attcttatta ataagcagat
      361 ttaatgatag taagcttttt aataagttga gaaatagtaa aactgaaaaa gcaataagtt
      421 ggatggataa acagggattt aagttattat ttatagcgta tgcctgtcca tttatgcctg
      481 gttgtttagt tactatcgca tcagcttttg gtaaaaaaga tttaaaagat tttgtgccag
      541 caatgttggc aggaaaattt gtaatgttta tagttataag ttacgtggca agtgatatag
      601 aaggttttat aacaagtcct ttaaagatag catcatttat attattggtg tttttgtcat
      661 ggaagattgg aaatagagta aataaaaatc ttgaaaatca taattatgat ttccatcata
      721 aaaaacatca caatgataaa gatgataaaa tgatttagct tgtacgaaat aaaacgtatc
      781 ttaaaatgat ttagtactaa attattttaa gatacgtttt attttttgta ttaaggtaca
      841 aaatttttag ttcttttgta aactaaaata tataaaactt atttatactt agttcaaaat
      901 attgttattt attttttcta caactataat aaaatataaa attgatatcc aatcaaaaaa
      961 ataactatga aatttaatag cttagatatg aatttttctg tttttgagtt agtttttata
     1021 ggtagtaaag aaatgtttat ggtaatggga taaaacaatt caataccttc tttagtaata
     1081 aggtcaagac acaagtgaga tagatatcct attataaacc cactggacaa acatgagaaa
     1141 actatattat tgttagtgaa gattgtcaga gatttaaaaa aactataaat aagtaataag
     1201 gcaattagac tgtgagtaaa ccctctatgt ctaaacctac ttccaaataa tttgtatatt
     1261 aacataaatt ttttacttat gtaagaacct ctcatatcta tatctggaaa taaagaacct
     1321 aaatatgcaa aataaatata aattataatt aatactattt tatatgcaat atcgtattta
     1381 actaggtaca gattgtttat aaaggggagt atcaaaagtg cgaatatata acctcccctt
     1441 tcatgtgttt cttttatcat ataaaaatcc tcctgaatcg tcactataaa cattatattg
     1501 gtaaatttag actatctttt attatattat atagcaaaca taagtttttt tcaaaaggat
     1561 tttaatattt aattaatctt gagattttaa atttttgtaa ttttctaaag caccattcca
     1621 atttactaca ttaaaccagt tgtcaatgta ttcatttctt ctattttgat attttaaata
     1681 gtaagcatgc tcccaaacat caagtcctat tataggagtt aggtttttac ttacagggct
     1741 atcctgattt ggagtagtca taatagataa tttgccatct ttagtagcta taagccaagc
     1801 ccaaccagaa ccaaagacat ctaaagcaga tttttggaac tcttgcttaa atttttcaaa
     1861 agaaccaaag tctctatcaa tagcttcttt taaagattca gaaggtatgg ttttttctgg
     1921 tgtcattata tcaaaaaaga atttatgatt ataagctcca cctgcattat ttcttacagt
     1981 tgtagcaata tctttaggta aagaatctaa attttgcaat aattcacata aagaataatt
     2041 ataaagctca ggatattttt caagagcagc atttaattta tcaacataag cttgataatg
     2101 cttatcatga tgcagtttca ttgtttcttt atctatataa ggttcaagtg catcatatgc
     2161 ataaggtaat ggtttaacct taaatttgtt attttcaggt gtaaaagcaa atgaagttat
     2221 gcactgtgag attataaata aagacataat aactggtatt aatatttttt tcttcataaa
     2281 aatcacctcc taatatagta tttctagaaa taaaaaaaat atgaaatatt aacagataat
     2341 ttataagaat ttaatagcaa tttcatttta aattgaataa gttttaaagc caaagacaaa
     2401 actatgaaat atagaataag actaaaggac agaaaggaat tttgattata tggacggcaa
     2461 tatttcaatt tattataaag gtgatatatc taaaaaagaa gatattaaat ttaaaataaa
     2521 agaatctgta aataatacaa gactttctag tagaatagag tataaggatg aggaaaaaac
     2581 ttttgatttt gaaagattta atgtaattga tgctatttat ggaaataaac catttgaaaa
     2641 agatggaaaa gttattgttt ttaatggaaa tatatataac tatcatgaaa taaaagaaga
     2701 attaattaaa aaaggacaca attttactac aaatgatgat attgaagtct tattagcatc
     2761 ttatatagaa tttggtaaga attgtgtaca taagattaaa ggaatgttca actttataat
     2821 ctatgataga gaaaatgaaa gtatatttgg agcaagagat ttatttggga taaaaccact
     2881 ttattatata aataaggaaa atgcaataat tttttcctca gagtataagt ttttacttga
     2941 atatatgaaa aatttaaata taaatgaaag aagcttacaa agttattttt catttcaata
     3001 tgtacttcct gaagatacta tgatacaagg aataaggctg ataccagcag gtcactattt
     3061 tagggtagaa aatggtatac tatctttaaa gagatataat aaacttgagt ttagaagtag
     3121 tacaaaattt ttttatacta aaaatcactt aggcaataga gatgtgaatg aggatgatat
     3181 aagaaatata gtagtagatt caatagtaac tcatatggaa gaggagaaag aaattggaac
     3241 atttttgtct ggtggaatag attcatctat tataactact gtggcttcac aaattaatcc
     3301 aaatataaaa tctttttctg caggctttag tgtcaaagga tatagtgaat tagaagtagc
     3361 aaagaaaaca gctgataagt taggtataga aaatatccag attaatataa cacaagatga
     3421 atatataaaa tcattaccga atgtaattta ttctttggat gaccctatag cagacccttc
     3481 agaagtaggt ctttatttct taattaaaga ggcaggtaaa catgttaaag tagcattgtc
     3541 tggagaagga gcagatgaat tatttggagg atataatata tataaagagt atagtactat
     3601 gaaatcagtt gtcaattcac ctacttatat taaaagtata ttaggaagag tttcagaact
     3661 tatgccaaac ataaaaggaa gaaattatct ttatagagct acaacaccat tagaaaaaag
     3721 atatatagga aatgcaaaag tttttgaaaa cagcgaagta aaaagattct tttttaagta
     3781 taaggaaaag aatatatatg aatacctgtt gtctaatttg tatagagatg cacaaaaaaa
     3841 taattacgat tatataagta aaatgcaaca tatagatgtg aatacatggc ttcaaggcga
     3901 tatacttcaa aaaattagca aactttctac agctgagcag gttgaactta gagtaccatt
     3961 tttatataaa gatgtatttg atgtcgcaaa aaatttaaga atggagcaaa aaataaataa
     4021 gaataataca aaagttttac ttagagaggc gtttagagaa atagtcccag aacatgttgt
     4081 tcaaaggaaa aaattaggat ttccaactcc aataagagtt tggttaaaag attctctagg
     4141 ggatattgta aaggaaacta tttataattc aaatgtagat gaatttatag ataaaaaata
     4201 tgcaataaaa cttttagata cgcatcttaa aggtcataga gataattcga gaaaaatatg
     4261 gactatattt acattttgtc tatggcatca attatttatt gagcataaaa atgtcgagta
     4321 ttaatacttt tttcaatatt aatcagaggg caaaagcatg caatgttttc accctcttga
     4381 ttattatgga ataggataag tagaattttg attgattagt ttactatctc aataaaatta
     4441 ttggcaaata ttttgacaag actactttca acagctaaat ttaagttttg tccatcatca
     4501 tatccagcgg aaatcaatcc tagtaagttt ccaaacatat ctaaaagagc tcctcctgaa
     4561 ctaccactag agattggagc tgtaaattgt atcatttgaa ccgtttcaaa atcacgaaaa
     4621 gctgaaataa ttccatctga aactgtatta aaaagaccta gagggcttcc aattgcaact
     4681 attttttgac ctctcacagg ttttttactg actttaacag gtattggctt gcattttcta
     4741 tcaaccttta tcacagctaa atcataatct gaatggtatt ttataatttg gtaagatgta
     4801 taaactttat catcattttc aaatctaact aaaaatgaat acccatcatt gactacatga
     4861 agattcgtaa gtatataacc ttcattattt attactactc cagaaccgct cttaaacggt
     4921 ttattttcgt tattgcacac aacaatcata acaacagaag ctgataactt tgcaaggtct
     4981 tcataagaaa gttctacttc ctcatcttgt acaatttttt tagatgttag atttgtgtta
     5041 ttaagtgtat ttttagagtt aggtttattc gtagtaggag aagtcttatt ggtattttca
     5101 ctgtaattta tatttgtaat atgcctttca aaaggtattt ctaatatatt tggagtcttc
     5161 ctagaaagtt caaatagagt agaaaatttt gaacattcag gatataaatc aagaatatct
     5221 ttatttggat ttatatataa aatatcacac ccaagttgtg ataaaaaata taagaaaaaa
     5281 agttcttgcc ttttaatatt tccaaagtat actatctttg gtgaaatagt agtcttcacc
     5341 ttgttataaa ataatttagg tagaaaatag tctatccaac acaaaagttt aactgcaaag
     5401 tttttgatga tagaggatgt acaatttggc atacttgtgg aaaataaatc taaagtattt
     5461 ttaaatgcaa tctttttagt ccaatttaag gtgtcatttt ctattttaca tggaaagtcg
     5521 atattatata aatcccaa
//
LOCUS       DAGSHT010000209         5533 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGSHT010000209 DAGSHT010000000
VERSION     DAGSHT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.215"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="MJB72_003190"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="MJB72_003190"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="MJB72_003191"
     CDS             complement(385..1569)
                     /locus_tag="MJB72_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY8264255.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="MJB72_003192"
     CDS             1942..4293
                     /locus_tag="MJB72_003192"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBY8264256.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="MJB72_003193"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="MJB72_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBY8264257.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2156 a          627 c          891 g         1859 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca tagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aagtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAGSHT010000210         5509 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGSHT010000210 DAGSHT010000000
VERSION     DAGSHT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.216"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(145..507)
                     /locus_tag="MJB72_003194"
     CDS             complement(145..507)
                     /locus_tag="MJB72_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264258.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1074..1715
                     /gene="sigK"
                     /locus_tag="MJB72_003195"
     CDS             1074..1715
                     /gene="sigK"
                     /locus_tag="MJB72_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HBY8264259.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(1812..3476)
                     /locus_tag="MJB72_003196"
     CDS             complement(1812..3476)
                     /locus_tag="MJB72_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBY8264260.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(3548..4795)
                     /locus_tag="MJB72_003197"
     CDS             complement(3548..4795)
                     /locus_tag="MJB72_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBY8264261.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(4782..5456)
                     /locus_tag="MJB72_003198"
     CDS             complement(4782..5456)
                     /locus_tag="MJB72_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBY8264262.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
BASE COUNT         1766 a          975 c          564 g         2204 t
ORIGIN      
        1 ccacttaaag aaatttcttt tagttgaatg tccttgtatc ctaactcaaa catattatta
       61 tgtgtataaa ttaaacctat acacataatg cacacagtac ttataataga aaatattatc
      121 aatgttatta ttaatataga gcctttattt tctagtaaca tagctattaa acctcagttt
      181 tttaaattca ttttttatct ctatatttat ttcatagcat tgataataat ctaaaatttg
      241 ctctatttta ctgttaattt catatccatt tatgtaattt ttttctatct tatcttcaat
      301 taactcatac ttactatttt gtattctata cttctcatct tctatatatt tttttgctgt
      361 acttaacatt tctctgtcag atatatttct actgataaaa ctataacaat tatacaatga
      421 tgtagaaact aaataaacag ccacatataa tatagcaata cttattatac attcaattga
      481 tataaaacca ctctttctta ttttcaataa taaaaacaac ccctaaataa tattttatat
      541 ataaacattt aactataagt tttttcataa atatcttttg taagtttttt cattgtaatc
      601 aaaatttctt cccatacata tataaattaa cacaccacct tcacccttaa cagtattaac
      661 tatgactgat acttttgctt gtaatcaact ttatacaaac aattaattac atttactttt
      721 ttaagtattg taaaattaca cctcctatat tcatattttt atagactcta atgctttgca
      781 ttaatctttt acttcaacac caaaacatat ataaatacta tttcctttta tatttattga
      841 cacctcaatt ccaatttata ctttataaat caataatttc ttcgccttat ttcatatata
      901 tacaacaaaa acttaaattt aaattcccac tctttatgtc atattccatt caaaagtttg
      961 caaatatatg ttttaaattc taaaaatatg catattattt tattagataa cagtcaactt
     1021 ggataagtct ccttcatata atttaaagtg accgttgaaa agggggtgac ctattggcag
     1081 ctcttaaatc ttttgaaaaa cccttaaccc ctgaagaaga gattgagtat ttaactaaat
     1141 ttaaaataga aaacgataaa agtgcaaaag atacattaat tgaaagaaac atgagattag
     1201 tagcttatat agcaaagaaa tataataatt caacggaaga tcaggatgat ttaatatcca
     1261 taggtacaat aggattaatt aaagctatag aaacttataa catagataaa ggaactcgat
     1321 tagcaacata tgcttctaga tgtattgaaa atgagatttt aatgaatatt agatctaaca
     1381 aaaaaaataa aactcaagta tctctacaag accctatcgg aacagataaa gaaggtaatg
     1441 aaataagtct tttagatata cttggtacag aggctaacta tgttcttgat gaagttgaac
     1501 taaaagttca agttggaaaa ctttacgaac aactaaacaa aattcttact cctagagaaa
     1561 gagaaattgt ccagttaaga tatgggttaa ctccttatgg ctataagaca caaagagaaa
     1621 ttgcacaaaa actcgatata tcaaggtcat atgtatctag aatcgaaaaa aaagctctta
     1681 aaaaactaga aaaagagctt gttcaagaaa gttaatacta tattttaaaa ggatatatcg
     1741 tacatcatta gacaaaattt tctatctaaa atcaaagtta cgatatatcc ttaaatttta
     1801 tagtacataa tttattttat actttcacat atttccttaa atataggtgt tgcagattta
     1861 tttccttttt gagttccttc tactaacaca gttattacat acttaggtct ttcttcagga
     1921 taaaatcctg ttatccatcc gtggtctata gcttttttat ttaaactact ttgagctgta
     1981 ccagttttaa ctccacaacc accttcaata tcttttaggt ctttagctgt accagtccta
     2041 gatacagact tcatatattc tttaacttgt gtacacacat aaggagaaat aagttcttct
     2101 ttttttgaag acttataagt ttttattgta ttcatattat tgtccacaag acttttatat
     2161 aagtatagtg gtttgaatgt tccattatta gctattattt gagtcatttg atttatttga
     2221 agtggtgtaa attctatatt ttcttgacct atagctaaat ttcttattga tatgtttttt
     2281 ggagcttctc taattttttc ttcatcaaga ccaatatcaa ccttttcaaa taaatgtaat
     2341 ttttcagcag atttgagtat cttttctttt ccaagtttca tagctatatc taaaaatgca
     2401 ggattgcatg aattagaaaa agcttgtctt aagttttgga aaccatgacc atctctttta
     2461 ttacatcgca gtatttcatt agtattccca actttagtct tacctgtaca gttgtaagta
     2521 tagttctcat ctataacacc attttcaagt gcagaaaata aaactaccat cttaaataca
     2581 gaccctggtg gatatgtcgc ttttataact ctattttcaa attctccatt ttttcctttt
     2641 aaactttttg atatgtcatt ttggtcaaaa ttaggtctag agcacattgc aagaatttca
     2701 cctgtagatg cttccgagat tattgctgct gtaggatttt cttctttatt tagtatttgt
     2761 tctaattttt tttgaatgtt ataatcaata gtagttttta gatgtctatc tttatcctta
     2821 ttatcaacag tttttacact tccctttagt atgtttagag atttttcatt tcctgcatct
     2881 ccagctttaa aagcacttat atacttttca tttgaatttc taagtacact atccatactc
     2941 ttttcaattc ctgattgacc tgatttatca tcttctttta tatatcctat tgtatgtgat
     3001 aataaaccat cttttgcgta cctcatagtt ttttcttcta ccattattcc atttttctca
     3061 agttcttttt tcatactctt atcgatattt ttagtctgaa tctgtattat agggcgtgtc
     3121 aattgctctt gtacagcttt atatatatct aaatcattca tcttagtagc tttcttaata
     3181 agctccaaaa ttttatagtt attatttaat ttttcttttt caacaattaa tacctgagat
     3241 ttacttgtat ctgtaagctt cttgttgttt ctatcataga ttattcctct tccactattt
     3301 aactcgactt tctcaacgct ttgtgattca acactttgtt tgtatttatc accttttaat
     3361 acttgtatat caactagcct ataaattaaa acactataaa ttattaaaat tatagtaaat
     3421 atacaccagc ttctctttcc cacttttttc aaaaatggag tcttcttttt tgacatgaaa
     3481 tcacccctta gataatttca ttattgacaa tttctaaggg gctaatacta tttttatcaa
     3541 ttttttatta ttcttcaacc ttttcttcta taacttttct aagcatataa tctttaccaa
     3601 cttttactga tagctttaac ttcactattt gtctaggatg aggagctgat tctatagctt
     3661 ctccttcttc attgtacatt tcttcaattt tagtaaacat agtttcttta tatggtccca
     3721 taacttctat ttcatcacca acacacattt tatttctttg ctcaacaaca ataagaccat
     3781 cttcatcttc tgtttctcta actataccta taaaatcata gtttctaaca tatgatgctg
     3841 attgataatt ttggtcttct gttgttggtt tattcaagta aaaacctgat gtaaaatgtc
     3901 tatgactacc tttttttagt tcttccatcc acattggatt gaacttccag ttttctgggt
     3961 ctctgtaaaa ttcatctatt gccattctgt atgctcttac aacagtagct acatagtatg
     4021 cagtcttcat tctaccttct atttttaaac ttgttatacc agatttgatt aactctggta
     4081 tatactctat catacataaa tcttttgagt taaagaagaa tgtacccctt tcatcttcgt
     4141 atattggaaa atactcacca ggtctttttt cttccaccaa atgatattgc catctacaag
     4201 attgagcaca agaacctttg tttgcatctc ttccagtcat atagttgctt attaaacatc
     4261 tgccagaata agaaatacac atagcaccat gcataaacgc ttctatatcc atatctaaag
     4321 gtgtttttgc tcttatctca gatatttcat caaaagataa ttctcttgcc actacaactc
     4381 tttttattcc atgctcgtac cagaagtttg cacttagata attggttgta tttgcttgtg
     4441 tacttaaatg tatttccata ttcggaatta cttttttaat gacactaagc actcctgggt
     4501 cagctattat aactgcatct attcctatct cttctagttc caacaaatag tcttctaatt
     4561 gtagaaaatc ttcattatga ggtattatat ttactgttac gaatactttc ttgcctcttt
     4621 catgtgcaaa agctactccc tctgccatat cttccttact aaaattcttc gctgcagacc
     4681 tcattccaaa aatttctcca cctatataaa cggcatctgc tccatatgtt atagctattt
     4741 tcaatctctc caaatcacct gctggagcta aaagttctac tttatttaat tccatctaca
     4801 tcacctcttt tactttcttt ataacttagt gctacaccat cacctattgg taccaatgat
     4861 gtgcttaaat agtcacaatt acatatataa tctaaataat ttctcattct tttaactata
     4921 gtcttttttc ttcttacaac aaaatcatca tgagcaacca tacctttata caaaatatta
     4981 tcagatacta aaagtccccc atcttttaat ttatctataa ctaaatcaaa aaatagctta
     5041 tattgacctt tggctgcatc tataaatata aggtcaaatt caccttggac ttgactaagt
     5101 ttttcttctg catctccttc tagtatagtt atgttattgt caaatccagc taattttatg
     5161 ttttcttttg ctttttctat cattttttca tttctctcaa cagttattat atctgcatct
     5221 tttcctataa tagttgcaaa aaaaatagaa gaataaccaa ttgcacatcc tacttctaaa
     5281 attctctttg gtttttgaac ctttaacaag acttttaata aatctgaaac ttctttatgt
     5341 attataggaa cactattttc ctctgcataa atttctaaat cttttaaaaa tccttctttt
     5401 tcttttaatg tcactcttat ataatcttca accaaatcat taactatatt actcataaaa
     5461 acactcccta aaataataaa tattcaagtt ttatattaat ttattattt
//
LOCUS       DAGSHT010000211         5484 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.217, whole genome shotgun sequence.
ACCESSION   DAGSHT010000211 DAGSHT010000000
VERSION     DAGSHT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.217"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1994
                     /locus_tag="MJB72_003199"
     CDS             <1..1994
                     /locus_tag="MJB72_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBY8264263.1"
                     /translation="VKNYKFERDITIDGVIGSNSTFFEVNKNWDIEEVLLHLNFSKSQ
                     ILNGDVSSLTVLINNVPIKSIKLNAKTNYKNTLEVLVPKDYIIQGYNEIKIKTYKTIS
                     DKICQDDSNTGNWMVIHKESYISIRYKQKKVENSINEYPYPYAEIENNHKLDTTIVVP
                     DNMTRGETTAVFNLASAFGKITKNDDLKLDVKLYSEMKNWSDDNIIYIGKPENTAEEI
                     LDILSIKEQTLLSSNCIIKQVDSPYNKNKKMMVVIGSNEDDLIKASNLLIENRLSNQV
                     LSSSVLVNKETNIKINREQKLNLGHLTLKDLGYSDFLLEGAFNQQALFDVKIPTGKVL
                     DDGSKIILNLRYSDNLDFEKSLVTVSINDVIVGSKKLDRSHSNNDKLELKIPKDIDNK
                     NYYQVKLTFNLSIKNSNCVTRESNNPWAYVSNNSYLALSTKENETLSFENYPYPFVRD
                     DEFNDLTVIMPDYSGSQAMTWMFRLGVTLGANINSHNGNINVIRGKEFSDKYKDTNIV
                     VFGVPHNNSVIKMLNNNLNIKFDKNYSNFISNDKISFIDDYGKNISTIQLIKSPYNNQ
                     KNIMVISSMNEKNLYLGMDYLLNKSKVNDLKGDTLIIDEYGEVEDLAYNLKSKKEVKD
                     SSWNMSINKTTKVFLMISFITIIVVMILSMLYIKKYKRR"
     gene            2031..3116
                     /locus_tag="MJB72_003200"
     CDS             2031..3116
                     /locus_tag="MJB72_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY8264264.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            3170..4294
                     /gene="wecB"
                     /locus_tag="MJB72_003201"
     CDS             3170..4294
                     /gene="wecB"
                     /locus_tag="MJB72_003201"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBY8264265.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENINSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            4372..5346
                     /locus_tag="MJB72_003202"
     CDS             4372..5346
                     /locus_tag="MJB72_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBY8264266.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYVDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         2312 a          510 c          919 g         1743 t
ORIGIN      
        1 aagttaaaaa ttataagttt gaaagagata taactattga tggtgttata ggaagtaata
       61 gtactttttt tgaagtaaat aaaaattggg atattgaaga agttttattg catcttaatt
      121 ttagtaagag ccaaatatta aatggagatg tttcaagttt aactgtattg ataaataatg
      181 taccaataaa atctattaaa ttaaatgcta agacaaatta taaaaataca ttagaagtat
      241 tagttccaaa agattatata attcaaggat ataatgaaat aaaaataaaa acatacaaaa
      301 caatatctga taaaatttgc caagatgatt ccaatactgg gaactggatg gttattcaca
      361 aagaatcata tatatcaatt agatataagc agaaaaaggt ggaaaactca ataaatgaat
      421 atccgtatcc atatgcagaa attgaaaata atcataaatt agatacaact atagtagtac
      481 cagataatat gactagagga gagactacag cagtgtttaa tttggcatca gcatttggaa
      541 aaataacaaa aaatgatgat ttaaagttag atgttaaatt atattcagaa atgaaaaatt
      601 ggtcagatga taatattata tatattggta aacctgaaaa tacagcagaa gaaattttgg
      661 atattttaag tataaaggaa caaaccctat tatcttcaaa ctgtataatc aagcaagtag
      721 attcaccata caataagaat aagaagatga tggtggtaat tggtagtaat gaagatgatt
      781 taataaaggc atcaaattta ttaatagaaa atagactttc taaccaagtt ttatcatcat
      841 cagtacttgt aaacaaagag actaatataa aaattaatag ggaacaaaaa ttaaatttag
      901 gtcatttaac attaaaagac ttaggatatt ctgacttttt gttggaagga gcatttaatc
      961 aacaagcttt gtttgatgtc aaaataccaa cgggtaaggt attagatgat ggttcaaaga
     1021 ttatcttaaa tttaagatat tctgacaatt tagattttga aaaatcttta gttacagtgt
     1081 ctataaatga tgttattgtg ggaagtaaaa aattggatag gtcacattct aataatgata
     1141 agttagaatt aaaaatacct aaagatatag ataataaaaa ctattatcaa gttaaattga
     1201 catttaattt gagtataaaa aattcgaatt gtgttactag agagagtaat aatccttggg
     1261 catatgtatc aaataattct tatttggcgt tatctacgaa agaaaatgaa accttatctt
     1321 ttgaaaatta tccgtatcca tttgttagag atgatgaatt taatgattta actgtgataa
     1381 tgccagatta ttcagggtca caagctatga cctggatgtt tagattaggt gttactttag
     1441 gtgcgaatat aaattcacat aatggaaata taaatgtaat tagaggaaaa gaatttagtg
     1501 acaagtataa ggatactaat attgtagttt ttggagtgcc acataataat tctgttataa
     1561 agatgttaaa taataattta aatataaagt tcgataaaaa ttactccaat tttatatcaa
     1621 atgataagat aagctttatt gatgattatg gaaaaaatat ttctaccatt caattaataa
     1681 aatcaccata taataatcaa aaaaatatca tggtaattag ttcaatgaat gaaaaaaact
     1741 tatatttagg aatggattat ttgttgaata aaagtaaagt taatgattta aaaggagata
     1801 cattgataat tgatgaatac ggagaggtag aagatttagc ttataattta aaatctaaga
     1861 aagaagtaaa agattcaagt tggaatatga gtataaataa gacaactaaa gtatttttaa
     1921 tgatttcttt tattactata attgtagtca tgatattatc aatgctatat ataaaaaaat
     1981 ataaaagaag ataatatgat aaatttatta ttttaaaaga ggtatataat ttgaggaaat
     2041 atatgaatga atttaaatta aaaagaagta ttttaatctc attaataatc ttaattttta
     2101 tatttatagt ttactttgta agtattgagt taaaagatag gagtctagta aataatcaat
     2161 ataaaggtaa gattaaatcg gccaatttat ctgtagatta tgaaataaat caagttatga
     2221 aggatataga taaattggga ttgaatacag taaatgtacc tataattata aatgtagagt
     2281 ccattaattc tgatattatg tcaatagata ataatagtaa agaaaaggca attaagttaa
     2341 taaaaaaatt aaataaaaaa ggtatttcaa caatattaga ggcatatcca tggattgaaa
     2401 atggaaaatt gtatgagact gactggaacc ctataaataa aaaaagattt ttttatactt
     2461 ggcaaaatgc catattaaat gaacttataa ttgatgtagc aaatccactg gatgtaaatg
     2521 ttttaaatat tggttctaat tttgtacatt tggaagaata tcaacaaaat tggggtgaaa
     2581 taatagattt tgtacaatcc aaatttgacg gtcttgtaac gtacagaact aattggtggt
     2641 atacaaaaaa agatgattta agtagtaagt tgttttatga acaaaagttg aacaataatt
     2701 tttttgataa gttagatttt atatctatag cagcatattt tgagttaagt aataaacctg
     2761 ttaatacagt ggatgaatta atttctgcct tacactcatc tactgtaaac aatagaggac
     2821 aaaatataaa acaagaaata tataaccttt ataaagctca tagaaaacct atattttttg
     2881 gagaattagg tttttcaaat agagaaagtg caagttctca gccgtggaat catactcctt
     2941 caaaagctgt aaatggcgaa gaacaagcaa ggtgttttga ggcatataag aaagtttttg
     3001 aaaatgaaga ctggataaat ggtttttctg tgttttgtgt aggtaaaata gatgacgaaa
     3061 aaaattttta cccatcaaaa gagagtataa aagttattaa gtcttggtat gaataatttt
     3121 gaaagtgtag aatttatata gattaaatag atgattagga gagatataaa tgaatgacat
     3181 caaggttatg actgtatttg gaactagacc tgaagctatc aaagtagcac ctttaataaa
     3241 ggaacttgaa aaaagagaga atataaatag tattgtttgc gttacagctc aacatagaga
     3301 aatgcttgac caagttatag aaacctttaa tataaatgta gactatgatt tggatataat
     3361 ggaaaaagga caaagcctga atgatataac atgtaagatt ttaaataaac ttcccttgat
     3421 tttaaacaag gaaaatccaa atataatatt ggtacatgga gatacaacca caactctagc
     3481 tacaagtttg actgcatttt ataataaaac tcttgttggt catatagaag ctgggcttag
     3541 aacctatgat aaatattctc cttttccaga ggagttaaat agacaactta caggtattat
     3601 tgctgatatg cactttgctc ctactaattt ggctagaaaa aacttgattt ctgaaggaaa
     3661 acctaataat aatatatttg taactggtaa tactgctatt gatgctttaa agatgacaat
     3721 aaaagaaaac tataatcatc ctataattga tgagattggt aacgatagga tgattttgtt
     3781 aacctctcac agaagagaaa atttgggtaa acctatgaaa aatatattca gagctataaa
     3841 gcgaatagta gatgactttg aagatgtaca gatagtatat cccatacatt taaatcctaa
     3901 aatacgaact atagctgatg agatatttgg aaaatttcct gaaaaaattc atattataga
     3961 gcctttagat gtagctgatt ttcataactt tttaaataaa tcttatatga taatgactga
     4021 tagtggagga atacaggaag aagctccatc attaggtaag ccagttcttg tgttgcgtga
     4081 taaaacagaa agaacagaag gaattgaagc aaagacactt aaattagtag gtactaatga
     4141 agatagaata tataattcag tgagcgattt attaataaat aaagataact atgttcagat
     4201 gagtaaagca tctaatccat atggagatgg aaatgcaagc aaatatatag tggatataat
     4261 aattaaaaaa tttaattgca aatatttaaa ttagataagg cctaaaacat taactaattg
     4321 atataagatt tatgataata ttaaggaata caatttttaa gggggagtaa tatggctaga
     4381 aaattaataa aaaatttggg taaaagtaaa agtgtaaaga gagtgaaact tttatttaag
     4441 aagattttta ttactgtatt catagtagca agtatagttg ctatttttaa tataacgaaa
     4501 tattttgagg aattatacaa agtaagggat ttaaaaagta ctaaaattga atactatgtg
     4561 gatgtggctg atgaagcagg agatggaaag gtccaattaa gttggaaggc cttacttgct
     4621 atagacatgg taatacatga tgaagattta agtaatataa aaaagaaaga cacattggat
     4681 ataggggaaa agtttatagt agaagataaa aatgataaag gcgaaaaggt gtataaggta
     4741 aaaaagttta ataaggtatt aagcgaattg aaatttgact cttctcaaaa aagtagagca
     4801 agaaaataca tgaaagattt agaatacaca taccttggaa ataaacaatt agatagtagt
     4861 gatgaaaaaa ttaaatttat aaagaagtta gaagactcag ctataagaga atatattgat
     4921 tatggaatat tgccctctat aacaattgga caagctatat tagaatctgg ttggggaaat
     4981 tctaaactta caaaacagag taataattta tttggtataa aagcagataa agcatggaaa
     5041 ggaaaaagtg tagaaatttc aacttcagag cattataatg aaaaaattgt agctagtttt
     5101 aggtcgtata attcattaca agattctgtc aaagaccaca gtttattttt aattaataat
     5161 aaaagatata gaaaacatgg gttgtttgag gcaaaagatt atattagtca agctcaatca
     5221 ttagaaaatg caggatatag tacagcagaa gataaaaaag gaaatcgtat atatgcagag
     5281 ctattaatag atgttattag gagttataat ttgcaattga tagataataa agttgagaca
     5341 aaataattgt taactttata tgaggagaaa acaagataag attcttttct acaaaaagaa
     5401 tagaaaaatt gtaaaaaaaa ctaaaaaaat gtattgactt gttatttaaa aggtgatatt
     5461 attattactg tactcgaaag agaa
//
LOCUS       DAGSHT010000212         5481 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.218, whole genome shotgun sequence.
ACCESSION   DAGSHT010000212 DAGSHT010000000
VERSION     DAGSHT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.218"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(15..116)
                     /locus_tag="MJB72_003203"
                     /pseudo
     CDS             complement(15..116)
                     /locus_tag="MJB72_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            411..932
                     /locus_tag="MJB72_003204"
     CDS             411..932
                     /locus_tag="MJB72_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8264267.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            1311..2486
                     /locus_tag="MJB72_003205"
     CDS             1311..2486
                     /locus_tag="MJB72_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8264268.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2521..4059
                     /locus_tag="MJB72_003206"
     CDS             2521..4059
                     /locus_tag="MJB72_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY8264269.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            4091..5293
                     /locus_tag="MJB72_003207"
     CDS             4091..5293
                     /locus_tag="MJB72_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY8264270.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
BASE COUNT         2098 a          625 c         1014 g         1744 t
ORIGIN      
        1 ttttataaag aatgttatat tttagacgta ttttttctat atttactggg agacatatta
       61 tattttttat taaataatct aataaaatgc tctacatttg gataaccaat actattttac
      121 tgataatctt ttccttatca ctaatcttac attttaaaat attattttta ctcgtaatat
      181 tatcataaga atttgtttct tatttaaaac taattagaat tataaagttt attgccatgt
      241 tattaataaa ataaatccag ttaaatcgag tgctagttaa agtaataaga aacttttaca
      301 atggtttcct ttatcgattg aaataaaagt taatatggta taatatctaa agagagaatc
      361 ttatcaatta gaatatgcat agaaacaagt ggcttaaaag gagacgttat atggaagaga
      421 tagtaaagaa ggaacaagca agtatgaatg atattttttt gtatcttaaa gctaaatcaa
      481 actcattata taaatttgta atattatata atgagtttat caataaacct aaagattatg
      541 gaactgggga acttataaac atggttgaag tacatatgct aacagcgatt gaagaaaatc
      601 caggaataag tccaactcag ttagctaaaa tatggaacag aactaaaggt gcaatatctc
      661 aaactatatc taaattggag aaaaagggtt atataactaa agaaaagaaa gaaggaaatg
      721 caaaaactat attactatat ccaaccacat caggagtaga gttaagtaag gcacataaac
      781 tatatgacac catagatgtt acaagaacat ttgaatactt aatcaaggag tgtacattag
      841 aggagataga tagtttttat aaagtaattg atttgtttat taatttatta gaagaatctc
      901 cgcaggaaaa taatgattct gaaaaagtgt agctgagatt gaagttgaat aaagagaaat
      961 agatattata gaagtagtac ttaagtacac attatataaa aatataatag gtataggatt
     1021 aaaaagttta gtggtttaaa gtttaaccac taaacttttt taatgtctat aatctactat
     1081 tatttaattt actaaaaata aaaaaactca ttatattaag ataaaaatag aaaaataact
     1141 agaaaaatac tataatatat cagcttaata cagaaaaaag ttaaaaaatt tgagaaaaat
     1201 aaaaatgaaa acgttttata ttgacataga gaaaaagtag tgataccatt taaatatagt
     1261 ttagtagcta aactatattt aaattataat aactatttta gggggatgtt atgaatttaa
     1321 aacaattaag tgaaaaatac gaagaatatg taattcaaat gagaagagaa tttcataagt
     1381 atccagaatt atcttttaaa gaaataagaa caacccaaag aataagggaa gaattacaaa
     1441 aaataggggt cccttttgta tctattgaac caaatatcgt aatagctact atacaaggca
     1501 aaaatgaagg taagacaatt gcaattagag gagatataga tgcgcttcca atgcaagaag
     1561 attcagatgt tgaatacaaa tcagaatatg atggaataat gcattcttgt ggacatgatg
     1621 ctcatggagc tatgttacta ggacttgcac atatgctaaa tgatataaga aaagatttaa
     1681 aaggtagagt attattagtt tttcaagcag ctgaagaagt tggtggtgga cacaaggaga
     1741 taatacagta ctttaaggaa aatggaggac cagatagatt aatagctact catgtttggt
     1801 ccgatattga agctggtaaa atttctgttg aaccaggacc tcgtatggct ggtgggtctg
     1861 gttggagaat agacataaca ggtagaggag gtcatggttc tagaccagat caaagcatag
     1921 atcctataaa gccagcttgt gatatagttt taaaagtatc ttctatacca gttaatcata
     1981 taagtgtact agaacaatgt gttgtacatg catgtgcaat acatggagga actacaatag
     2041 gaaatgttat tccaaacaat gcagaggtaa taggtggata tagatatttt actgaagaat
     2101 caggcaaaaa agtgtttgac attataaagc aaatgagtaa tggtgttgga caagtttatg
     2161 gagtggatgt gaaggttact catactggag gcataggacc tgttatcaat gatgaagaag
     2221 cagttaagat ggctaaagaa atagtttcta atatagatgg attggagtta gattcttatg
     2281 agccaatatg tgcatcagat tgttatggtg aaatattaaa agagtattct ggcttttatt
     2341 gttatctagg ggtaggtaac aaagaaaaag gaataatata tgcacaacac catcctaagt
     2401 acaatataga tgaagataca ttaaagttag gttgcgaatt tatggctaaa tatgcagttg
     2461 aatttttaaa taaaaataat aactaaaata aatcgaattt tataaatggg ggggatgaaa
     2521 atgcaaggtg cattaaagga aaataagaaa aaaagtggtg gaggattttt aggatggatt
     2581 gaaaaagttg ggaataaaat gccacatcct ctagcattat ttacatatat tacattgata
     2641 gtagtagcat tatctgcaat agcaagcttt ttaggttttt cagcaacaag tcctgctaca
     2701 ggaaagttaa taaaagttat gaacttaata agtgcaaaag gtttagtact atttatgcaa
     2761 gagtttgtta agaacttcca aaacttccct gtgcttggag tagttttagt tatggcagtt
     2821 gccacaggag tttgtgaaaa gacaggattt ttttcaactg ccataaaaat gagtttatca
     2881 aaagttaagg ggaatgcggt tgtatttata atagctttta taggagtatt tgctaatcaa
     2941 gctggtgatg ctgcttttgt attagtacca actcttgcag gagcaatatt ttatggatta
     3001 aatagaaatc cattagctgg tatattctgt ggatttgcat cagttggtgg tggatttgca
     3061 acagctgtaa ttcctggggg atgggacgtt acgctaacgc caataactgt atcagctgct
     3121 caaactattc aacctgcatt tgatatgaca ttattagcta gttattatgc actatttgta
     3181 tctgcgttta tagtagctac agtatcaaca atagtaacgg ttaaatttat agaacctaaa
     3241 ttaggtaagt atactggtaa accagaaggt atagatgata atcaaggttt tgaggtcaca
     3301 aaagaacaag caaaagcagc aaagttagca ggttttacag ttttagctta tgtagcgtta
     3361 ttaattgctt tgtgtatacc agctaatagt tttttaagaa gtccagaagg ttctttaata
     3421 tttggagcac cacttatgtc ttgcttacag ttttttatag taatactatt cttcttgcca
     3481 gggattgtgt atggaatgag ggttggtaaa atcaagactg taaaagactt aaatgatata
     3541 ttatgtcaat caatggcagc aatggcaccg tttatcgtac ttgcaatagt tataggtcaa
     3601 ttcttaacgc tattctcagt gtctaattta gcacaggtac tagctataaa aggtggagca
     3661 ttactcaact cattgccact accaccacaa gctataatac tgctattctt agtactagta
     3721 gcgtttataa atatatttgt tataagcgga agtacaaaat ggatgatatt tggaccagta
     3781 tttgttccaa tgttaatgca attaaacata catccagcat ttactcagtt tgtatataga
     3841 ttaggagatt ctgtaaccaa tcatttatca ccattaaatg cattctttat aatactttta
     3901 gcaacagtcc aaaaatacga taaaaatgca ggtatgggaa ctatattctc agctatgata
     3961 ccttacacta taggttactt tgtagtactt gcagttttag tagtagtttg gatgacaata
     4021 ggtataccag taggaatagg tggacaagtt tggttataaa acatatttaa taattttggg
     4081 aggaaagaaa atggaaacaa ctgaaataag atacaatttt gatgaagtaa tagatagaag
     4141 tggaacaaac tgtcttaaat atgatcttaa gagtcaattt atgccagaat ctccagaaga
     4201 tgcactacca ctatgggtag cagatatgga ttttgcatgt gcccctgaga taattaatgc
     4261 aatgcatgaa agaatagaca gaaaaatatt tggatatagc tctcaacaaa ctgatgatta
     4321 ttatgaagct gtatgtggat ggtttaagag aagatttgat tggaaaatag aaaaagagaa
     4381 tatattcttt agcccagggg ttgtgcctgc gttaggattt ttcacacaaa tattaacaca
     4441 aaagggtgat ggtatcataa tagaaaagcc tgtatactat ccttttgaag ataaaataac
     4501 taataatgaa agaaaagtag ttaatagtcc tttaaagttt gaagatggtc attattctat
     4561 ggattatgat ggatttgaag aattagctaa agatgaaaat aataaggtgt ttatattatg
     4621 tagtcctcat aatccagttg gtagagtatg gaaaaaagaa gagcttagaa aaatagtaga
     4681 catatgtaag aaatatgatt tgtggataat atctgatgaa atacattgtg atattattcg
     4741 taaaggtgaa aagcatatac cattagaagt agcatgtcct gattataaag atagaataat
     4801 aacttgtaca gctccaagta agagttttaa cttagctggt atgcaattat caaatatagt
     4861 aatcaataat aaggaattac aaaaaaaatg gatgcttgaa acagattcta aattagctat
     4921 attcctacct aatccattcg caatagttgc tactaaagtt gcttataatg attgtgaaga
     4981 ttggatgaat caagtaaatg agtatataga tggtaatatt aaatatgtag aagattttgt
     5041 taatgagtat atgcctaaag taaagataat aaaatctcaa ggaacttact tagtttggtt
     5101 agactttaga gggtatggat tagatgcaaa acagcttgaa tatgtaatgc ttaacaaggc
     5161 gaaggtattg ctagatgaag gatacatatt cggagaagaa ggagcaggat ttgaaagaat
     5221 aaatgttgct tcacctagaa caattataga agagtgtatg aatagaataa aatgtgcatt
     5281 tgaaggatta taatagtaat atctaaataa tatatttgtg tattttaaaa aaagactatg
     5341 ctaaaaaaat atttcaaagc atagtctttt aaagttttat gatgagttta aaaaatttca
     5401 aaacgttact attcacttat atgtttatat tagaaataat tccacaatat actcattttc
     5461 taaagttgca cttttataca c
//
LOCUS       DAGSHT010000213         5477 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGSHT010000213 DAGSHT010000000
VERSION     DAGSHT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.219"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..186
                     /locus_tag="MJB72_003208"
     CDS             <1..186
                     /locus_tag="MJB72_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8264271.1"
                     /translation="SREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            405..2882
                     /locus_tag="MJB72_003209"
     CDS             405..2882
                     /locus_tag="MJB72_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8264272.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRILIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            2897..3583
                     /locus_tag="MJB72_003210"
     CDS             2897..3583
                     /locus_tag="MJB72_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264273.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKDVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TIIVSDGLIV"
     gene            3857..4690
                     /locus_tag="MJB72_003211"
     CDS             3857..4690
                     /locus_tag="MJB72_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264274.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            4728..5189
                     /locus_tag="MJB72_003212"
     CDS             4728..5189
                     /locus_tag="MJB72_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBY8264275.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRFLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            5220..5468
                     /locus_tag="MJB72_003213"
     CDS             5220..5468
                     /locus_tag="MJB72_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY8264276.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2084 a          598 c          883 g         1912 t
ORIGIN      
        1 tcaagagaag aagcatcaaa tttgtttaat cgttattata gaggaagcaa tacaaaacaa
       61 aaaatagaag gaactggttt aggactagca ataacgaagc aaattattga agttcatgga
      121 gggaatatat ctgttgaaag tatacctgga ttaggtagct cattttatat cacctttcct
      181 atttaattaa ggttaattaa ggttaaataa aaaatatatt aaggttgata gattattata
      241 tttatatagt agtttatcaa tcttttttat ttaaggggtg atttgattaa aaatattata
      301 aatagaatga taaaagtatg gaggagtcta ttagagattt aatttagatt aaagtataaa
      361 aatattagat tgaaatatct tagataaggg aagaaggtgt aagtttgaaa attatattaa
      421 agtatatttt cacaaatatt aaagaacgaa aaattagaac agcagttatg ttattgtcca
      481 tagttttatc aacagtttta ctttttgttt cattctcaat tggattatct tatgaaagtg
      541 cacaaagaaa aatggcaaaa ggtatgtatg gtacagcgac tatatctgtc caaagtaaaa
      601 atccagatat attaaccaat ttagaagaca tacctgattt aaatactata aaatcaaagg
      661 ttggtgtatt ggaaagctct gctatttata ataaaggggg atactatgaa gaatttagtc
      721 ttatatcagc agacttaagt caactaaata aaatcaacaa gccacgatta gaaaatggcg
      781 atagtattac tgatttttct ggtgataaaa ttattcttcc aaatcgcttt acatcaaaat
      841 ataaaataaa aaaaggtgat tctattactt tgcagattta tggaaagtct tatacttttc
      901 aagttagtga tattgcatct tatgatacag tatttttgag gaacacaaga ggtgtaaatg
      961 cactgctacc caaagagact ctatcaaaaa ttataaataa aggcagtgga tatacaagaa
     1021 ttcttataga gtcagaagaa gaaattacag aaaatctagt aaataaatta tccaaagaac
     1081 tttctactga aaaatatatg gtatcaaata ctataaatga aactaagata atttcagatg
     1141 ctagacaaaa gacaatgcct ttctttttaa taagtttttt tgcattaacc cttagtatat
     1201 ttataatata cagcagttat aaggttatta cattagaacg tcttcctttt attggaacat
     1261 ttagaagtat tggtgccaat gaaaaaactg taactcgtat attgatgctt gaaagtatat
     1321 tatacggaag tataggagga ttaattgcta ttcctattgg tgtagttgtt ttaaatttaa
     1381 tgcttcatgg gttaggaaat tctctcgaac aaggaatatc aattccagta gtaatatcac
     1441 ccattggagt gattatatct gttatagtag caataatcgt ttcttcattt agtgcatata
     1501 tacctgttaa aaaagctagc catttgccaa taaaaaatat tgtactagga acagtagaag
     1561 aaaagaatgt ttcaaatcgt tctatacttt ttataggaag tataatgttt attttatcta
     1621 ttttacttcc aagaatatcc cctgaaaata cattgtatct agctggtgga ttttcattac
     1681 tgggattaat tgtagctact attgtgttga ttcctcttat tactgatatc atgtctatag
     1741 tttttgagtt tgtttataaa aatattttag ggaatgaggg taaattagct gctagaaata
     1801 tgaaaaataa caaaaatata atacagaata tcactttact atttataagt atttctgcag
     1861 ttattgcaat aagtgttgtt ggtaactttg tcaaaactta tataacagat gtgtttcgtg
     1921 acgcagaatt acaaggtttt gcagatggaa agatgaatga agagtttatt gaagatgtta
     1981 gacatatgga tggaattaaa aaaatattac ctctttatgt tatgaataat gagatttctg
     2041 gtaatggtgt aacattatca agactagagg gtacagataa tataaaacta tataactcta
     2101 tgtttggaat caattataca aattttgaga taaaaaaaca agtaatagaa gcttttaatg
     2161 ataaacgttc tgttattttg aatgaagata ctctaaaaaa agtaggctta tctataggag
     2221 atacaataac tctatctaat gataagtatg atttttctta taaaatagta ggtagtttta
     2281 aatcccgtgc aaatgatgta gaagctgtta taccatcaca ttatgcagtt aatgattttg
     2341 ataaaactaa ttatggtttt ttagtatata cagcagtcaa tccagatgct gttatgattc
     2401 agataagata tttatttgga gatacatata attggagtcg tacagttgaa gaatttaata
     2461 atgattcttt aaatactata agtagttttt tgtctcctat gaataagatg acctatttca
     2521 tttttcttct tgctacagta ggtattatta ataatctact gattaattat attcaaaaaa
     2581 gacgttctat agctatgtat aaatctatag gactaagtga taaacaaaat ataaaagtaa
     2641 cattgattga agggtttact tcaggtttac ttggagctgt tattggtatt gtgatatcta
     2701 tacttgagat tcaaactatc tttatagttg caggaccaaa aatttcaatg aaaccagatt
     2761 tagattttaa aacatttatt attgttgggt tattgggaat tatagtaact ttaataggtt
     2821 cgatagtgcc tattataaaa gggaagaaaa tgaaactgat agaagaaatt aagtttgaat
     2881 aattatggag gttaatatga aaattacaca taatagttta gctgtagaag caaaaaatat
     2941 tatcaaagaa tataaaattg gaaatacaac aactcgagtg ttaaaagatg tatctttaca
     3001 agtaatgaaa ggtgaatttg tttctatcat gggtcaatct ggttctggca aaagtactct
     3061 tttatatatt ttaggtggtt tagatactcc aacgagcgga aaagtgtata tgaatggagc
     3121 tgatatatca cattttaatg atgagaaaat gagcataata cgtcgtagaa atataggatt
     3181 cgtatttcaa ttttataact taataccaaa tttaaatgtg gaggaaaata taatgcttcc
     3241 tttgttatta gatggtaaaa atttaaaaga ttataaaaat caattagatg aaatcttaga
     3301 tatagttggt ttaactgata gacgtaaaca tacaccacga gagttatctg gtggtcagca
     3361 acaacgtgtt gctatagctc gagctttaat tggaaaacca gagattttat ttgctgatga
     3421 gcctactgga aatttagata gtaaaacagg gatagaaata atagatttac tgaataaaat
     3481 aaacagagac aatggacaaa ctataattat ggtaactcac tcaccagaag cagccaaaag
     3541 tagtagtcgt actattatag tttcagatgg tttaattgta taaggactaa ttattgtatt
     3601 tgagataagc atttttaatt atataaatta gacatatgta aagtggatat aagttaaata
     3661 ctataagtgt gctataatat taaaataaca gataaaagta ctatattttt tattgattat
     3721 tttaatttta tttgtttatt aaatatctaa tgagtattca tactatctct attattaaaa
     3781 gcagttaatg atatatagag ataatatgga ttcttcttac tttaataaat attcacgata
     3841 ggacaggata taatatatgt taaaaaaaga aagtattcat tttataaata aaaataggtc
     3901 acttatagtt tgtgaagaca attttgttta tctcttgcct tgtatacaat taagaaattg
     3961 gatatcgaat tttactatat cgtttccaaa tcaaacaata atttctgata attacacgat
     4021 tattcctcat ggaagtgtaa ctttggtgtt tttttatgat gccacagggt tacatagtct
     4081 attatttggt ccaactacta aaccaaaaac agtaggaaat attgctaata gatgtgatgt
     4141 tatactaatt atagaatttc aacctgctgg ttttttcccg cttataggta tacagcaaag
     4201 tgaacttatt gataaagttg ttccattttc tataattaat acttcattag atttagaaat
     4261 taaaaaaata tttaatgaat ctttatctat agataaattg attttaaagt tggaagaatt
     4321 attgatttct aatattaaaa ttgaatactc atatgagttt attttggcaa ttcagttgat
     4381 tattcaaaac tctggaaata tatcttcaca agaaatttct aaaaaagtat tttatagttc
     4441 taggcattta aacagactct ttaatcagta cctagggttg agtatgaagt cattttctcg
     4501 tttggtacgt attaataaat ctataaaact tctaaacaat aataaaactt cattgatgtc
     4561 tatatgtaat gaattaggat tctatgatat accacatttt attaaagatt ttaaaattgt
     4621 ctgtggaata actcctcaaa aataccgagc taatatgtct gatttttaca gtgaaattgc
     4681 aaaattttaa gttataatac ttataacaaa gaaaggatag tgataagatg aaatttggag
     4741 gagtattgct aattgtcaaa gacataaata aatcaaaaaa gttttatgaa gatgttatgg
     4801 agcaaaaaat cacattagat ttaggagaac atgtatcatt tgaaaatgga tttggattac
     4861 aaagtaatta taaagctata attggatatg atttaccaat atatcaacaa tcaaataact
     4921 ttcagttgta ttttgaggtt aatgacatca aacaatggga aagtaagata aatagaatag
     4981 gaaatattga atttttgcat tatataaaag aatacccatg gggtcaaaga acttttaggt
     5041 tttatgattt tgataaaaat attatagaaa tttcagaaag tatggaaagc gtcataaaga
     5101 ggtttttaaa acaaggttta gctttagaag aaatttcaaa acgcactatg tatccagttg
     5161 aatttattac acaatttcaa caattataaa atattaaaat atataatttt ttctttatat
     5221 tgcaaaaaac aaaggcttgg ggcataatag aaccagaaaa acctgaatct caacagcaaa
     5281 tggctcatat ggatttcgct gttaatgatt tagaagaagc agttcaatat gcaattcatt
     5341 gtggagctac aatcgcagag gagcaattta ctgatgactg gagagttatg attgacccag
     5401 ctggacaccc tttttgctta tgtcagatga aatcaattat ggaaagtagc catttttcat
     5461 tgttatagac caaaata
//
LOCUS       DAGSHT010000214         5452 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.220, whole genome shotgun sequence.
ACCESSION   DAGSHT010000214 DAGSHT010000000
VERSION     DAGSHT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.220"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            194..691
                     /locus_tag="MJB72_003214"
     CDS             194..691
                     /locus_tag="MJB72_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBY8264277.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(868..1116)
                     /locus_tag="MJB72_003215"
     CDS             complement(868..1116)
                     /locus_tag="MJB72_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264278.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(1182..2198)
                     /locus_tag="MJB72_003216"
     CDS             complement(1182..2198)
                     /locus_tag="MJB72_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY8264279.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(2256..3023)
                     /locus_tag="MJB72_003217"
     CDS             complement(2256..3023)
                     /locus_tag="MJB72_003217"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBY8264280.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(3091..5280)
                     /locus_tag="MJB72_003218"
     CDS             complement(3091..5280)
                     /locus_tag="MJB72_003218"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBY8264281.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         1683 a         1032 c          532 g         2205 t
ORIGIN      
        1 tttcaacata ttaaattatt ttatcttata tttttctctt tagtttcaca catcaccaag
       61 tctaaacttt atattatata ttaatatttt tgacttgtca agttatttat gttataatat
      121 taatcattag ataattaaag cattgtacct aagttgcaat atctaaaatt caatttggag
      181 gtataactag catatgtata aaattggtga aagtgtaatg tatccaaaag agggagcctg
      241 ctctgtaaat gatattgtaa caaagaaaat aaatcatgag atgcaaaaat actatgaatt
      301 aagtgtcatt tttaatagta accttaaaat ttcgattcca gttctaaatg cagatagaat
      361 tggcatacgt ccagttatgg atggaaatga tgttgataat tttatccaat ctattaataa
      421 aacagatggt gtatggattt ttgatagaaa agaaagactt aaactgtacc aagataaatt
      481 tcattcagga gatgtttttg aaattgtaaa attaatcaaa atgcttatga tacaagacag
      541 ttcaaaacaa ttgtgtagca cagataagga gtttttaaat aaagctcaaa aatttgcttt
      601 atcagaatta gctgctgctc aatgtaaatc ctatacaatg gtattagagg aaatgaaaaa
      661 acatatatta aattcaaaga atataaatta aaccagtttt ttaaactggt tttatttttt
      721 atactaatca tccatgctac tttagcttat tttaaactgc ctaatctaat attaataaag
      781 ttatctcaat tagatagatg tattttattc aaataaaaaa aaactaagag taaatcatat
      841 tactgattta ctcttagcta ataaaattta atcttcttca agttcttttt tctcttgtaa
      901 taatacactt aattcatttg cttctaaaac ttgtgcctct gcaatatcaa gtaactcttg
      961 ttgccattcc acacatttat ctttgtaatc aaaaggtagc gttctgtcaa ctatgtactt
     1021 atcaatacag tcctccgcac ttttaattat ttcttttctt tcatcaataa atttttctaa
     1081 ttctactaag gcttcttcat aagcttttct actcatataa attccccctt tgtacaatta
     1141 ttctacatta aaatgatttt tcctcttttt tgtataaaat tttatttgta tataactttt
     1201 ccatcaatat cttttatagt ttgacatcca gttaatatca tagaactact tagctctgat
     1261 tgaagtttat ttacataagt ttctacacca tcagttgcac caccaaaaga agcagtcaca
     1321 aaaggtcttc ctattaatac tgcatctgca cctaaaccta tcatttttag tacgtctaaa
     1381 ccagttctca ctccaccatc tgcaagtatt gtaactttac ctttaacagc atcagcaatt
     1441 tttggtaaaa cttcacatgc tcctggtgta cagtcaagca ctcttcctcc atgatttgat
     1501 acgactattg catccacacc tgcttctact gccattaaag catcttcaac agtcattata
     1561 cctttcaata taaatggtag tttagtagac tttactaact cctttatttg tttaacattt
     1621 ttaggaagta ctggttttcc atgtaatgat aaagttacta gaccacaggc atctatatca
     1681 actccaactg caaatgcacc agcttcttca gctaatctta tcttttttat aatattttca
     1741 ttatcccaag gtttaataaa tacaattcca gctccattat actttttgac tacatctaaa
     1801 ttttccatta aaaaagcatc tactgccgta tcaccaacca ttgcataaat accactatta
     1861 gcacatcctg caactactgg ttctatgtaa tctctttcat taatttttcc acccatattt
     1921 aaagtagttc ctgttactgg agctgcaaat ataggtgcac tcattttctt tccaaacatc
     1981 tctatagatg tatcaggatt tgatacatca tgtattactc tcatatttac ttttactttt
     2041 tctaaactct tgctattttc tataaaagaa gaaccacttc ctttacctcc catgccaggc
     2101 acttcacctg cacaagcaac cccattacaa accttacaaa ctttacaact tccatttaaa
     2161 ttttctctag cactttttaa taattcatta tattccatct ttaatccccc attaactata
     2221 gtaatttata ttatatacat tatatcatat tatatttaca gtatattttt caaaaaactt
     2281 tttctgtgga taggagttat gccatatttt tcaatagctt catagtgttc tttagttcca
     2341 tatcctttgt gagatttaaa cccatattct gggtatactc tatcatattg gtacattata
     2401 ctatccctag taactttagc aagtatactt gctgctgcta ttgatataga ttttgaatct
     2461 cctttcacaa ttggattttg agatatgtca atacctggta ttgttgctgc atcaactaat
     2521 aaataatccg gtgctttttt taaacaattt atagcctttt tcattgccat atatgtagca
     2581 tttaatatat taaattcatc tatttcttca ttgtttacta tacctattcc ataatctagt
     2641 gcttcttctt ttattacttc aaaaagttca tctctttttg cctcactcag tttcttagag
     2701 tcatttacac cttctatttt ggtatctttt ttaaatacta caacagaagc tacaacaggt
     2761 ccagcaagtg gtcctcttcc cgcctcatca attcctccaa tatataaata acctttatca
     2821 tagccttcgt tctcaaatat atttatagtt tccaatcttt cttcttcttt tctaatatta
     2881 tccagctttt ttgctagttt tatagctaga ccttgtacag attttctttc atcaactctt
     2941 aacagctcta tatattccat atatttttct acttctaaag tctctattat ttctttaatc
     3001 tccctcacac ttttatcctg cataccattc cctcatcttt cgtttttaaa aatttaattt
     3061 ttaaatatat taaaattttt taattagagt ttaattgcat ccactatagc cacattttaa
     3121 acatatacta catccacctg tatgtgcaat tccttcactt ccacactcag ggcacacaaa
     3181 tttttcatct tcttcaaaat ttttatttat tatttctttg gctttttcat ctttattatc
     3241 tttttctttt ttattatttt tataatattc ttttttacta ttttcttttt tatcttcttt
     3301 attattttct tccttttttt catttattaa aattccactc cttttacatc catctctaaa
     3361 tattgttact cctttaagac cagcctccca tgccttaata tatatattga aaacatcttc
     3421 aactgtagtt tcaaaaggta tatttattgt agatgatata gaagcatcaa tatacttttg
     3481 ccatactttt tgcatgttta ttctttcttc atatcctata ttcatagcag taacaaaaaa
     3541 atctggtaaa cattcttcat ctgtaatacc tttcttgtcc atatactctt taacaattgg
     3601 agtatatact ttataataca catcctcatc atgcaagctt tcagtttttc ttatatatga
     3661 aagattaaat ataggttcta ttccaccact gatacctatc attgtagata tggaacctgt
     3721 aggaggtatg gttaaaagtt gtgaatttct aagcccatac tttttaacca tttccttagt
     3781 gtcttcattt agattttcta taaaaaactt agatttagaa atattttcat atttatactc
     3841 actaaatgtt ccatattctt tagcaattaa tgctgattgc ttcacagctt catttaacat
     3901 agtcttacca attaaatcac atagttctaa agattcatca gaaccatatc ttattcccat
     3961 ttttataagc atatcagcta tacccattac acctaaacct atctgtctat acttatgtac
     4021 actatctctt tgttcttgta atggatgcaa gttcaatccc tcatctaaga catcatttaa
     4081 agctactaca gcttctctaa cgcaagattt taatttatta atatcaaaag tagcatgttc
     4141 accaaatggt tgtattataa actctgacaa gtttattgag ccaagtagac aacttcctcc
     4201 acttggaagt ggttcttctg cacaaggatt tactcctgaa aagcaaaagt tcttatcttc
     4261 actcataaaa ttatatgttt ttattttatc ccaaaacaat atacctggtt ctgcataatc
     4321 ccagttattt ttcacaaatt tattaaataa atcataagca tttactatct taactatttt
     4381 ttctccagta gtttcaactt caaaagtaca attatataac tttttttctc ttacagcttg
     4441 cataaattca tctgttactc ttacagaaat atttgcttta gtaaccttct ccaaatctgt
     4501 ttttatatct ataaagtcct ctatctctgg atgatttaca tccatagaaa tcataagagc
     4561 tcctcttctc cctctttgac caattaagcc agttgtcata ctgtaaagtt ccataaaaga
     4621 gactgctcca gtcgtatgtt ttgcagcatt gttaactttc gctccttttg gtcttagctt
     4681 tgaaatatca attccaactc ctccaccata actaaaagtt ctagcaagtt tttttgcagt
     4741 atcaaatata tcttctatgc tatcttttgg tggttctatt acataacaat tagaatatgt
     4801 tacttttttt ccttctttct gtaatcctct atttgctaaa attcttccag caaatagaaa
     4861 tcttttttgt cttattagtt ttgatattct tgaactatta tttgaaattc tctttatcca
     4921 ttcatcaaaa ctctcaccat tatgtctata cttgttcttc catattgatt tttgtaattc
     4981 atcaaactcc caagctttag tccttatttc agagcgtctt tctctatata atatgtagtg
     5041 ttttgctata tcttttctat ttgattccat aagtagtact tcaactatat cttgtatttc
     5101 ttctacggat aagcattttt ccatagaatt atagtcatta gctatttttc ttgatatatc
     5161 atatgccaac tctctatcta ctccaccttc actatttata ctagctttat agacagcatc
     5221 ttcaatctta cttttactaa aaagttcttt tgtaccatct ctttttatta tgtacttcat
     5281 ttttaaacct ccaataaatt agtattaaat taatacacac attaactata actctaaaca
     5341 aactttttat atatttgtat attttcatat taattatcta acataatata tattatacta
     5401 ttttatattc aatataacac tccttatagt tttcttaact tgtaaaatat ct
//
LOCUS       DAGSHT010000215         5450 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.221, whole genome shotgun sequence.
ACCESSION   DAGSHT010000215 DAGSHT010000000
VERSION     DAGSHT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.221"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..465
                     /locus_tag="MJB72_003219"
     CDS             <1..465
                     /locus_tag="MJB72_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBY8264282.1"
                     /translation="ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNI
                     LSLQGNLYNVIEKSNKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDY
                     YEGIMFRGYYPNSYKEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            487..1113
                     /locus_tag="MJB72_003220"
     CDS             487..1113
                     /locus_tag="MJB72_003220"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBY8264283.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1147..2196
                     /gene="hisC"
                     /locus_tag="MJB72_003221"
     CDS             1147..2196
                     /gene="hisC"
                     /locus_tag="MJB72_003221"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBY8264284.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2199..2777
                     /gene="hisB"
                     /locus_tag="MJB72_003222"
     CDS             2199..2777
                     /gene="hisB"
                     /locus_tag="MJB72_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBY8264285.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGQAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            2779..3384
                     /gene="hisH"
                     /locus_tag="MJB72_003223"
     CDS             2779..3384
                     /gene="hisH"
                     /locus_tag="MJB72_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBY8264286.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            3381..4103
                     /gene="hisA"
                     /locus_tag="MJB72_003224"
     CDS             3381..4103
                     /gene="hisA"
                     /locus_tag="MJB72_003224"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBY8264287.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4085..4843
                     /gene="hisF"
                     /locus_tag="MJB72_003225"
     CDS             4085..4843
                     /gene="hisF"
                     /locus_tag="MJB72_003225"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBY8264288.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            4871..>5450
                     /locus_tag="MJB72_003226"
     CDS             4871..>5450
                     /locus_tag="MJB72_003226"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBY8264289.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEIS"
BASE COUNT         2209 a          474 c         1093 g         1674 t
ORIGIN      
        1 gaattaaatt taaataagaa ttgtgaaaat caaattaaaa atttactata tacgaagaac
       61 actcatgaac taaaagtata tattgaagaa ttaaaaatta agcgtgaagt aaaagagtta
      121 ttatcaaata ttttaagttt acaagggaat ttatataatg ttattgaaaa atcaaacaag
      181 ttctattgta ataataagat gaagcaagct cttgaagaat taaagcaagt aaataacttg
      241 attgaggaat gtaatttttt agataaggct agatttgatt tatcaatgat tactatgctt
      301 gactattatg aaggaattat gtttagagga tactacccaa actcatataa agaaattctt
      361 agtggcggaa gatatgattc tttaactaaa gagtatggaa aagaaattcc agccattgga
      421 tttacactaa gtgttgatga gttaatgaag tatgtatata agtaaagttt agaaggaggg
      481 attgttttgg attatttgac tatagcacta gcaaaaggac gaattgaagg ggaatcattt
      541 aaaaaattta aaaaaatggg tctaggagat agtatagaca cagatacgag aaaactaatt
      601 tttaaagatg aagaaaataa aattatatat atacatgtaa agccatcaga tgtagttaca
      661 tatgttgaaa aaggagtcgc tgacctcggt atagcaggta aagatactat tttagagaat
      721 gagacagatg tatatgaaat atatgattta ggttttggaa aatgtaaatt tgctgtagca
      781 ggattaaagg gagatagcat atatagagaa gatgaatatt taaaagtggc aacaaagtat
      841 cccaatatag caaaaaagta ttttaaagag aaaggtcaaa aaattgaaat aataaaatta
      901 aatggttctg tagaactagc tccaatagta gggttgtcag atgtaattgt agatatagtt
      961 gagactggca atacacttaa agcaaatggt cttgaaatat tagaagacat atgtaatata
     1021 agtgctagaa ttatatcaaa tagagctagc tatagattta aatatgaaca aattcagaat
     1081 ataattcgtt tatttgaaga acttgataat taaaatataa ttgagcttta taaggaggga
     1141 tttaagttga gagaaaaaga aagtataaga gagttaaggg gatatgaacc aaatcacgta
     1201 aactgtaaag taaaacttga tgcaaatgaa ggaagtaaga ggttgtttaa ataccttatt
     1261 aaagaaattt cagatagtga tatagattta aatttatatc ctgaagattc ttatagtgat
     1321 ttgaaagaat ctataataga ttacataaat atttctggag taaataagaa aaatcttcta
     1381 gtaggaaacg gttcaagtga aataattgat ttaataatac atacttttgt agataaagat
     1441 gaagtaatat tgagtttctc tccaagtttt agtatgtatt ctatatatag tcagataaat
     1501 gggtctaaat ttataggtgt tgaaagtgat gaaaatttag ttataaatat agataatgtg
     1561 attgaaaaag taaaggagaa taatcctaaa atagtaattg tatgtaatcc taataatcca
     1621 actggaacaa tattaaagag ggaagagata ataaagcttt tagattcaac taatagtcta
     1681 gtggttttag atgaagcata tatggatttt ggagaagaaa gcatgttaag tgatgttttt
     1741 aagtatgata atctaatagt tcttagaact ttatcaaagg catttggatt ggctggaata
     1801 agaacaggat atatgttatc taatagtagt ttaataaatt ctgttgagaa agtaagacct
     1861 ccatataatt taaattcatt atcagacttt attgctacaa gagcacttag aaataaagat
     1921 gtggtaaagg cttatataaa ggaagtaaaa gaggaaagag aagttttata tgaagaaatg
     1981 attggtatgg gaattaaagc atacaaatca caagcaaatt ttatattatt ttattctgaa
     2041 atcgaaaatt tatcacaaaa acttattgat agaggtgttt taataagaaa atttggtgga
     2101 aagttagaaa attattacag agttacaatt ggtgataaag aagaaaattc tatgtttgtt
     2161 ggagctataa gagatatttt gaaaaaggag aaataggtat gagaatttgg aaggtagaaa
     2221 gaaatactct tgaaacacag attttagtag aattaaatat tgatggttct ggtaaagctg
     2281 aaatagatac tggaatagga tttttagacc atatgcttac cttaatgtca tttcatggca
     2341 agttcgattt aaaagtgatt tgtaagggtg atacatatgt agatgaccac catagtgtag
     2401 aagatatagg tatagctata ggtcaagcat ttaaaaatgc actaggtgac aaaaaaggaa
     2461 tacgaagata ttctaatatc tatataccta tggatgaatc tctatcaatg gtagcaatag
     2521 atataagcaa tagaccttat cttgtattta atgctaaatt tgatacccaa atgattggta
     2581 gtatgagtac tcaatgcttt aaagaatttt ttagagcttt tgtaaatgaa tcgagagtaa
     2641 cattacatat caatctctta tatggggaaa atgaccatca taagatagaa agtattttta
     2701 aagcatttgc aagagcttta aaagagggta gtgaaatagt ttctaatgag atagcatctt
     2761 caaagggggt tttgtagaat gaatataata gtagactatg gtcttggaaa tatagattct
     2821 gtgtctaggg gttttagaaa agctggaata gaaactaaaa tttctagtga tatagatgaa
     2881 ataaaacagg cagattcact tatacttcca ggtgttggag cttttagaga ttctataagt
     2941 gccttagata aactaggatt gataccaata ataaaggagc atgtttctaa gggtaaattt
     3001 atgataggta tatgtctagg aatgcaatta ctttatgaaa aaagttatga gtatggagaa
     3061 tatgaaggat taggacttat aaaaggaagt attgataaac tggatataag tttaaaagtg
     3121 ccacatatgg gatggaataa tctaaaattt aataaggcaa atgatgatat acttaaatat
     3181 ataaatgaag atgattatgt atacttcgtt cactcatatt atgcaaattc atcaaatgaa
     3241 gaactaattg cattttcaga atatgaaaag aaaatccctg ctattgttag aaaaggcaat
     3301 gtatatggta tacaatttca tcctgaaaaa agtggagaag tagggttaaa tatattaaga
     3361 gcatatgggg agatgataaa atgataatat ttccagcaat agatataaaa gataataagt
     3421 gtgtaagact cacacaagga gaatttgaca aagtaaatgt atattatgat aatccattag
     3481 aagtagcata taaatggaaa aatgaaggag cagaatatat acatatagta gatttaaatg
     3541 gtgctagaag tgaatttggt gtaaatacaa aaataattga ggacatagca aacaatatag
     3601 atatacctat tcaagttggc ggtggagtta gggataagga aaaagtcaaa agtttaataa
     3661 atgctggtgt tactagagta atattaggta gtatagcgat tgaaaatttg aatttagttg
     3721 aagaattggt caatgaatat aaagagaaaa ttgtagtttc tatagatgct aaagatggaa
     3781 aagtagcagt taggggatgg gaagttgtaa gcaatgtaga ctcattaaca ttatgcaaac
     3841 aacttgaaaa gataggtgta caaactattg tttatacaga tatatcaaaa gatgggatgt
     3901 tgcaaggtcc aaattttgat atatatgaaa gaatagcaaa ggaaacatca ctaaatgtta
     3961 ttgcttctgg aggcgtaact tctatagaag acgtaaaaag actaaaagct atgaacttat
     4021 atggtgcaat aataggtaaa gcgctttatg ataaaaagat agactttaag gaggcacaac
     4081 aattatgctt actaggagaa taataccttg tctagatgtg agaaatggaa gagtcgtaaa
     4141 gggaaaaaaa tttaaagata tagtagatgt tgatagtcca gaagtacttg gaaaatttta
     4201 tagtgattgt ggtgcagatg aacttgtatt ctatgatata actgcatcaa atgaagagag
     4261 aaagacatct ttagaatttg tgacaaaagt tgcagaaaat ataaatatac cattttgtgt
     4321 tggtggagga gtaaataaat tagaagattt cactgatatt ttaagaaaag gtgctgataa
     4381 agtttctata aattcatcag cagtcaaaaa tcctgaatta ataagggaag cttcattaaa
     4441 atttggagca caatgtgttg ttttatcgat agatgcaaag aaaaatgaag agggttcatg
     4501 gagcgtatac gtaaaaggtg gaagagaaaa aactaatctg gatgcaattg aatgggctgt
     4561 taaaggtgta gaacttggag caggagaaat cgttgtaaat agtatggatg aagatggaat
     4621 gaaaaatgga tatgatatag aattattatc aaagataact tctttagtaa atgtacctgt
     4681 tatagcatct ggaggcgctg gaaaaaagga agatttttat gaggcggtta ataaatccaa
     4741 tgtagatgga attttagcag catctgtttt tcattttggc gagataaaaa taaacgattt
     4801 aaagaaatat ttaaaagaca tgggtgtaga agtaagatta taagttaaat actaaaagga
     4861 gattagaaat atggataata agtgtaacaa tgtatacagt gatgaagtag agaaatttat
     4921 aagaagtata aagtttgatg ataaagggtt agtccctgtt gttgttcaag aggtagttag
     4981 caaagatgta ctgatgttag catatatgaa taaagatgct ataaagaaaa ccttaaaaga
     5041 taaagtagct tgctatttta gtagaagtag acaagaactt tgggttaaag gtgagacatc
     5101 tggtaatact caaaaagtag ttaagatgtc ttatgattgt gatgtagata ctatactact
     5161 ttttgttgaa caaactggtg tagcttgtca tacaggcaat tattcttgtt tttacagaga
     5221 cttgtttgat gatagttcaa aaatggagct tgaagtacaa acaaatatat tgaaagaatt
     5281 atatgattta atcaatgaaa gaaaaaataa tccagttgaa ggttcttata ctaattatct
     5341 ttttgaaaaa ggaatagata agattcttaa aaaagtaggt gaagagagta gtgaggtaat
     5401 tatagcatca aaaaataccg ataaaagtga acttatttac gaaattagcg
//
LOCUS       DAGSHT010000216         5443 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGSHT010000216 DAGSHT010000000
VERSION     DAGSHT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.222"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..289
                     /locus_tag="MJB72_003227"
     CDS             <1..289
                     /locus_tag="MJB72_003227"
                     /inference="COORDINATES: protein motif:HMM:NF016060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBY8264290.1"
                     /translation="LLSNSLSWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSC
                     RLFSGFIEILLMYLMIDLFSLNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(354..2471)
                     /locus_tag="MJB72_003228"
     CDS             complement(354..2471)
                     /locus_tag="MJB72_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY8264291.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(2502..3209)
                     /locus_tag="MJB72_003229"
     CDS             complement(2502..3209)
                     /locus_tag="MJB72_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY8264292.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(3316..5187)
                     /locus_tag="MJB72_003230"
     CDS             complement(3316..5187)
                     /locus_tag="MJB72_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBY8264293.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
BASE COUNT         1577 a          980 c          581 g         2305 t
ORIGIN      
        1 tttattatca aactctctat cttggtctat agcagtttta ttttcatata taacaaacaa
       61 attttttgta ttcaaaagtg aaaaaactga gtttttattt ctattaaaag aatttgtatc
      121 ttttttatct tgtcgtttat tttctggttt tatagaaata ttacttatgt atttaatgat
      181 tgatttattt tctttaaatg atatcttagt aaaaataggc actaatataa ttgtgataat
      241 tttaaacttc gtcttcagca aggtagtagt ttttaagcat agaaattaac attattatat
      301 ttatctttat gcagaataag gagttgccta aaaaaaacaa ctccttatta gctttaagaa
      361 acaatttcta ttttatctgt tgctccccat ttttcaactt tattattttc tcctgatgct
      421 aattctacat aaagtccatt taccttattt ccttctaaaa ttatatcact attagaagta
      481 cttttccaac tggagtctat ttcatctcct acattcatag cttctggttt tgtagtcttt
      541 attctatagt aaactttatt accattgtat ccagttacac taatagttac tttagaattt
      601 tcatcaacag aaacagttga ttttattcca tttgctacta ttttaaaacc atcatctgtc
      661 gcaccagctc ttccccatct tgaaactttc tttcttgtat aatcaatttc aacaacttct
      721 atatatgatc cattcttagc atttactttt aatggttccg tatcttcaac caagtcccaa
      781 tcatttatgt taattctatc accaaccctc atagctgatg gtttatatga caaaactcta
      841 taataaattt tataatttga atttttaggt ttctgaggta cagttaacat aatttgatta
      901 ttctcaagag gagaacatgc acttatcttt ggagataatc ctgctgctgt atcactatca
      961 tcatctttat ctgtattatt agtgttaggt ttattgccac ttattcctgg ttctttattt
     1021 ccactaacag tatcaatctt tcccgaattt cttatattag tacttgaaga atcattatct
     1081 atctttgtta tagttccatc atttcttata tttacatctt ttgcagaact aaatattgtt
     1141 acaatccata tttgtccatt attttcattt tcaatcttgc aaccattatc atcagaaatg
     1201 tccatttgat ttatatatcc tgaattagtt aaagttccat cttttatatc tttaagtatc
     1261 aatgttttac ttatatcacc tgtattattc aactctccat ttggcatatt tatagttatt
     1321 gactgactat attctccata taaatcaacc tttatagctt tttcagtctc taatttaaaa
     1381 gattcactta tttttttatc aggtctaaac tttataatat cattagcatc cactttcttt
     1441 aaagcagaat ccaattcttc aattgtttca actgtatatt tagtttcttc ttgcatatcc
     1501 aatatatctt tatagacatt ttcatttcct attccaccaa cctgagtaac tgtactaaat
     1561 atcttagtat ttagtacatc tttctgaata gaatcaattt tatttcctac taataccact
     1621 ggagaattgt tttttgcagc caacactcca actgatagtg aatctattaa atcactttgt
     1681 tttttcattc catccttggt tatatatgca tttcttaaat ctgtttcttt ataaaattct
     1741 tttataacat tagcatttgt ttcatttcta ttatttcctg atatcctctt agaatttggt
     1801 aaattttttt ctatttcatt agaaatggaa tttaatcctc caataatata agaatttttt
     1861 atattttggg cttttataaa tttattagct acttcaactc caaggtttga atctgaaagt
     1921 ataattggca tgtaattttg agctgctatt gaaccaacac ttacagcatc tgctaatccc
     1981 ttttctccat ttactacaat tatatcagaa atatcttttt cattattcaa tttttcagcc
     2041 aattttaagg atgtttcata tctatcattc ccaaaaattc tttcaactgt tattttctct
     2101 gattttaatt cattttctac atttacacct agtgcatttg aacctcctac taaaaataca
     2161 ttttttactc caagcctttt tagttctgat tttgtcctaa aatccaattc tttacttcca
     2221 gatagtaaaa taggtgcatc tttcaattta gcaaatggag ttgcagataa tgcatctgct
     2281 attgagttat cattcactaa tacagcatta tcagatctta accaaccgtt ttggcttatt
     2341 tttattgctg tttcccatct acctgaacct attaaactgt ctttattaga tgcatttacc
     2401 acattactag gtaaaacaat tgataaaaat gctgctgtta ataaagatat tatttttttt
     2461 gatatttgca ttaattatct cctcctaatt taaatataat tttatggagc atgtacatac
     2521 tgtgttttat ttaattctaa atcatcaaaa tgagatttta ctgaatggta tcctatagca
     2581 taatgtccac tttcaggatt ccatttctta tatgcattca tcgcacttaa taacttctcc
     2641 tcttgactag gtaatacctt ccagacttct acatttggat taactttttt aaaatcctta
     2701 tagtcatatg gttctacata aaatcttaaa tcttttattt ttccttctct atataactct
     2761 agtgcagctt ctcctaatgc tctatgatct tcatgtattc cactagcttt aaacggagta
     2821 acagttttta cttttgcttc tggatattct tctaagtatt taagtattat ttcctttgcc
     2881 ttttctttgc ttaactcacc atcatgagct ctattatctt ctatatgtat attactttct
     2941 tttacaccca ttgcttcaca gctatcctta aattcttcat ctctatcttt tgaaaattca
     3001 ctttctgata aagagtattt atgaacatct ttaatatgat aagaacatga ccctccatca
     3061 tccaaaactt ttcttattat agatttacta ccatctgtgc acaatatgac atgtgtatca
     3121 cttttttcaa catgttcaat tatagcactt cccatactta aggtttcatc atcttgatgt
     3181 ggtgtaaaaa acaaagttgc atctaccact actacatcct cctactttat aaaatgttat
     3241 aatttacaat aaatagcata attatagaaa aagattcctg tattaattaa ggaatcttct
     3301 ccacagtata agttattacc aacctagtat tttagctatc ttttgtatta ctgtcaattc
     3361 aactccatta cctatttgag ttacttctgc accaagattt aattttttac taagtgcatt
     3421 cttttgtgag tctgttatat cagttttagc tagaagtatt ggacctttac ctgctgctag
     3481 aggtcctgct gccaatgcat ctactagcat accattattt ccatatccat cttttgctat
     3541 atatagttta tctaatttgc cataatatgt ttctataact ttagcattag tatcgtgtct
     3601 tgtttctcct tcaactcttt gaacttttct atctttatca atatctgcaa gttgattttc
     3661 aatttggcta gatacattat tagagccacc aactatgcca atctcttttc catccatcaa
     3721 tttgatagca tctgctgata gatcattcca accatttact attataggag ccttaagttc
     3781 agcagcttta gcagctatac tcatagcatc agcctcccct tttgcgccta ctacaaatgc
     3841 atctttaaaa gaccccatag cttttgctac tgctacagaa gtcatatgtc tatcatctcc
     3901 agctagtctt tcaacattct tagttactga ttctaattgc tttttagctg tatttgatac
     3961 tgctgattct ccacctacta tatatatttt agctgatggg cttttcttaa ttatatcttt
     4021 tatataatct aaagttactt ttggtatttc attatcagat gctaataata taggtgcttt
     4081 tttagattga gcaagtggtg tagctgctaa tccgtcaact agtttatttg aattaactat
     4141 aactatattg tcagtatatt ttgtttgttt tgctatagcc actgcagttg catatctatc
     4201 atctccagct atcaatttaa cctttgaatt accttctaat gcagctttgg catcttttaa
     4261 atctttttca ttggtacttt ctacagtaag ctctgttgct tttccagatg catcagtata
     4321 cttaatagta aatttatatt ttcctgtttc tgaatctttt gctgaaatat cactaattga
     4381 ctttgatgta ccatttgcta ccatattacc tttaacagtc aatctatatc catcatataa
     4441 atcttgtggt gaaactgtat ttaggtcact attttttata gttatttcgg ccaattggac
     4501 atcatcaata tttttagctc ctgctacacc tagtttatcg attttattta ctctaaatcc
     4561 ttctgcagaa gggtctaaat ttgttccagt tgaatcaact ggatttgctg ttaaatcaac
     4621 tttttcatca ccaataccta catatatagt tttagaagtt actgtttctg ttttatctct
     4681 taaagttata actaacttat ctttagcact atcatatgaa gcttttacat ctgcaacttt
     4741 ataaattcca ttggtttcag ttttagcttt ttctgttata gaattagcct cagcggaaag
     4801 gtctgctgaa ttttcatatt tttcagttgc ttctgcaact acttcatttg cactatttgt
     4861 tatatgtcct ttatctgtta tagttacaat aagttcttta ttttctccca aagcatttac
     4921 taatttctct aattcatcta tattagttat tatttttaat tttgataaag tctcaccaac
     4981 ttttatttca tatattggtt gtccaacttg agaacctcct gtatactttt gagacattaa
     5041 acctctaact ttcgaaacta attctgtagc ctctttctta gttattgttt ctttttttac
     5101 ctgtgtagtt tctgcagcaa aaactggagc tactgatgta cttatcattg cagcagccat
     5161 aattacagaa agattttttt tattcatggt attttccccc taaaaaattc aaatttttat
     5221 tattttttcc ttatttacca attataatat atatttgata ttatttcaat aaatgagact
     5281 tattgtaata attaaccaaa agatataaac tttttcttat attttatttt tttaatattt
     5341 acgtatacaa aaaagtaaca aaattgtaat tttttttaat aatccagttt ataactaata
     5401 gacttttcat cttctttata gtataatatt aaatggaaga aag
//
LOCUS       DAGSHT010000217         5415 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.223, whole genome shotgun sequence.
ACCESSION   DAGSHT010000217 DAGSHT010000000
VERSION     DAGSHT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.223"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            364..1806
                     /locus_tag="MJB72_003231"
     CDS             364..1806
                     /locus_tag="MJB72_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY8264294.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1848..2201
                     /locus_tag="MJB72_003232"
     CDS             1848..2201
                     /locus_tag="MJB72_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264295.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2392..3213
                     /locus_tag="MJB72_003233"
     CDS             2392..3213
                     /locus_tag="MJB72_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBY8264296.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            3471..5354
                     /locus_tag="MJB72_003234"
     CDS             3471..5354
                     /locus_tag="MJB72_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBY8264297.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IRIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMKWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
BASE COUNT         2167 a          517 c          953 g         1778 t
ORIGIN      
        1 tattcttaat aatattgata aaagatttac ttaaaaataa tatagatcat gtaaacatat
       61 aatctattta ttgtatacaa taaaatttca ttttgtaaaa cagtgaatta aatatgtaat
      121 atagatatca atttttatta caaattgtta ctaaatatag gtacattaga acaaaaatct
      181 agtaaattgt aactaaaatg taaatttttc ctttaatcta tacaatatta tggaaacttt
      241 ttgtaattta tgatataata acaaatatat aattattaaa ttataataaa tgactatagc
      301 gtagatgtaa tactatgtta tagtcatttt tgaattaaac ttgatatatc aagggggaag
      361 aatatgagaa caattagaag agttttagca ttaggtttaa cattagctat atttctaata
      421 aatgtaccta atgtagatgc attaacatca gatacaatta aaggtaacaa tatatatgag
      481 acggctggat taatagcaga taaaaagagt tatgatacag caattatggt aaatatggat
      541 aattctatag cagatggtct ttcagcaagt ggtcttgcag gtgctgttga tgctccaatt
      601 ttgcttgcac aaaaaaataa gataccaaat gaaacaaaac aaagattaaa aaatgttaaa
      661 aagatataca taataggtaa agaattatca ataagtaagt cagtagaaac tgaactaaaa
      721 aacacagggg cacaggtaac tagattaggt ggagatgaca gaataaagac tagttatagt
      781 gttgctaaag aggtaaatgg tatcaaaaaa gttgatgaag tgatattaac taatgcatat
      841 aaaggagaag cagatacaat aagtgctgca cctgtatcag taagagatat agctcctata
      901 gtacttacag atggaaagag tgtgcctttt tcaacaagca atgtaaaaac atatgcagta
      961 ggtgggagta tatcaatgag cactagttta gttaataaaa ctaatgctaa aagacttggt
     1021 ggttcagata gatatgatac aaataagaaa gttataaaag agttttatcc agatgcatca
     1081 gaattttatt tgagtgatgg atatgattta gtaaatgcac ttacaggttc tacaattgct
     1141 aaggaaaatc caattgtatt ggtgtcagaa agtagtgata agtctatatt agcaggagca
     1201 gataagatta ctagattagg ttcaataagt gacagtgtgt ataataagtg tgtttctgct
     1261 gcacaaaata atggcgattc gtctacaaaa ggtgagtcac ctatgaaaaa tgagacaagc
     1321 atattaggtc agccaacagc tagtttagaa gcgtgcttaa aatgggcaaa atctaaaaaa
     1381 gctaatgatt tatttataga gttaatacca atattatatg atactgctgt tcaggaaggt
     1441 gttaatcctg ttttggcagt agctcaatct gcaaaagaaa ctggtttttg taattttggt
     1501 ggagtattag atgcatcatt taagaatcct tgtggactta aaacttctgt aggtggttct
     1561 gatactgata agaatgccca ttcaagattt gatacttggg aagaaggaat attagctcaa
     1621 attcaacatt tatgcttata tgcaggacaa gatggttacc cactttcaaa tccagtagac
     1681 cctagacatg aaaaatcttt atttggtaag gctaaaacag ttgaaagttt atctaataat
     1741 tgggctggag gtcaatatgg acaagattta gtaagaatga tgggagaaat tgaggcaaca
     1801 aaataattga agattatcaa atatccaata aataaggatt gattattatg aaaaagagta
     1861 atatttttag tatcatattt cctacagtca taatggtgct aatgttttta acttttgcta
     1921 cagatatatt taatattcca gatattcaat ctaaaagtat ttttgttgtg ggaatggttt
     1981 tgatttttcc aatctctttt ttgatacaag ggataatttg tgtattaaat aagactaatt
     2041 ggattttatc tcttattgtg tctttagtaa cttatatagt attaatgtat atatttttaa
     2101 atgattctgc atatatatat gttttacttt atacagcttt ttatatgatt ggacatataa
     2161 cagctaaact ctattacaaa attaagactt ttaaaaatta aattgaagta tataaaagta
     2221 acattaagag aagtatcctt aatgattttc taaaatcaga aataggtgct tctctttttt
     2281 gtaaaaataa aagtatgtaa ataaaattta accattataa ttttctttta acaaatgtat
     2341 acaataaact tgttgtataa ttaagataga taaaataaag ggagggataa tatgaattta
     2401 atagataatc atattcatac taatttttca agtgatggaa aggattccat ggaagataca
     2461 ataaaaaaag ctataagtat tggtgttaga tatttgactt ttacagacca tttagaacat
     2521 gatgaagata gaggattttg cataaattat aatgactatg ttccagtatt taataagttt
     2581 aaagaaaaat ataaaaagga catagagtta ttactaggtg ttgaagtggg ttacagaaaa
     2641 catctaaaaa atgaaataga agaaataata aattctaatc cttttgattt tgtattgtgc
     2701 tcaactcata caattgacaa tgtacctgtt ccatcaaagg agtattttaa aggtcttagc
     2761 aaagaagatg cgtattataa atattttaat agcatacttg aaactaatcg tgaatttgga
     2821 gattacaata tatatggtca tttggattat atatctaggt atggaatata ttcagataat
     2881 agagttatat acaatgactt taaagatgtt attgatgagg tcttaaagtc aataatcaat
     2941 aatgggagtg gtatagagct aaatacttct ggttatagat atggactaaa tgctatacat
     3001 ccaaatgaag atattctaaa aagatataaa gaacttggag gatttatagt tactgtaggt
     3061 tctgattctc atagggtaga agatatatgc aaagattttg atgtggccta tgatatgctt
     3121 aagtatcttg actttaaata tgtatcttta tttaaagaaa gagaaactta ttttgtaaat
     3181 atagaaaaag ttaaatctaa caatatagct tagagtgtgt tttgaagtaa attaatggat
     3241 agttaaaaag ttaatagtta taatattgta tcctctagaa aaatactaga ggtttttatt
     3301 ttgctaaaat aactaaatag aattaaattt aaatagtata gtttatttta aattgaatag
     3361 tttattgttg atatatttta ctattatata tttttattgt gatataatag taaaatatat
     3421 taacctatct ttagattata taaattatta taaaggaggg aaattttaaa atgtctaata
     3481 aaaataaaga aaatataact ttaagtaatc aaatcaaagg aacaattcaa aatagtttaa
     3541 caaaagaagc tatattacat ataccaatga gcaattatgc ctatggttat gatagagaaa
     3601 cactacatat aagacttaga actaaaaaaa atgaagcgaa aagagtaatt ttacgaatag
     3661 gagaccaata tgtgtgggat aaaggaggtg caggaggagg aaatttaaat gcatctggag
     3721 ttggttggtc tggtggaaca aatatagtaa tgagtaaaga agtagaaact gaattatttg
     3781 actattggat agctaagtgt aagcctttaa ataaacgctc acgatatggt tttataatag
     3841 aaggtcaaga ggaaaaactt ttatttacag aaaaaagaat aatagagttg ggaaatgaaa
     3901 atgatgaaaa ggaattatgt gaaataggta atttttttgg atatccatat ttaaattata
     3961 tagatatacc taaagtgcca aattgggtga aagatactgt ttggtatcag atatttccag
     4021 atagatttgc aaatggtgac ccgtctataa acccagaagg agtagataaa tggggagcta
     4081 ttccaaccag agataatttt acaggtggag atttacaagg tgtgatagaa cacttagatt
     4141 atttgagtga tttgggaata aacggaattt acttttgccc tattacaatt ggaaaaacca
     4201 atcatagata tgatacggta gattatatgg aggtagaccc tacattagga gataaagaga
     4261 ctttaaaaaa actaatagaa gaagcgcata agagaaatat aagaataatg ttggatgctg
     4321 tttttaatca tataggatat tattctaaac agtggcaaga tgtagttcaa aataaggaaa
     4381 attctcgata taaaaactgg ttttatataa aagatatgag taaaattgat actccaatag
     4441 aacaaataga tgaaaaaaat ataccttatg aaacctttgg gtgtgaaaaa tatatgccta
     4501 aacttaatac agaaaattca gaagtaataa aatatttatt ggatgtaggc aagtactgga
     4561 tacaggaatt tgatatagat gcgtggagat tagatgtttc aaatgaagta gaccatgttt
     4621 tttggagaaa gtttagagaa gaagtcaaaa aagtaaaacc agacatttat atattgggag
     4681 aaatttggca tggtagttta ccatggctaa tgggtgacca atttgattca gttatgaatt
     4741 acttgatgtc agaagccatg aaaaaattct tttgtacaga tgagataaac gccgaagaat
     4801 ttaagtatat gataaatgat gtaatggtaa gttatccaat acaagttagt gaagttattt
     4861 ttaatttact tggaagtcac gatactacaa gaatattgac ttatgctaat ggaaatattg
     4921 atagatttaa acttgcatat atgtttatgt ttgtacaaac aggatgtcct tgtatatact
     4981 atggtgatga aatcggaatg gaaggagaat taagttcaat atctgaaggt caaagaaaat
     5041 gtatgaaatg gaatgaagaa aaatggaata aggatatatt tgattttatg aagaagatta
     5101 ttagacttcg taaagaaaat aaagaactta ggtctatatc aaatgaatgg gtgttagcag
     5161 ataaggaaaa tggaacgatt atattgaaaa aagaatctat ttctataata ataaataatt
     5221 catctaaaaa tataattttg acgttaccag attatttagc aaataaaaaa gtaaaagatt
     5281 tatatgaaga agaaatagtt gatttaaaag aagaactaat gttagagaaa tacaagttca
     5341 ttattttaaa ataatacaaa aaagtattta ttttagattt catgttaaat tattatacag
     5401 tttaaataag tcttt
//
LOCUS       DAGSHT010000218         5344 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.224, whole genome shotgun sequence.
ACCESSION   DAGSHT010000218 DAGSHT010000000
VERSION     DAGSHT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.224"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..244)
                     /locus_tag="MJB72_003235"
     CDS             complement(<1..244)
                     /locus_tag="MJB72_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264298.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLF"
     gene            complement(271..942)
                     /locus_tag="MJB72_003236"
     CDS             complement(271..942)
                     /locus_tag="MJB72_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264299.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLKERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(954..3530)
                     /locus_tag="MJB72_003237"
     CDS             complement(954..3530)
                     /locus_tag="MJB72_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8264300.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKMGQCEIFSNLNESKLSDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMVGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFMIIVLSMSIGGIIVIKDNYKYSFLDVQNKSVQEQTYM
                     NADFILSNVYLNETGNNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NKLDYYEAINSKPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYTFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLIYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISEREFKNMILYEGILYGALSSIITIVSGLI
                     IQLKMYYMQGFVSHGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            3738..4673
                     /locus_tag="MJB72_003238"
     CDS             3738..4673
                     /locus_tag="MJB72_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY8264301.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKKGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQNRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            4692..>5344
                     /locus_tag="MJB72_003239"
     CDS             4692..>5344
                     /locus_tag="MJB72_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264302.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIR"
BASE COUNT         1826 a          735 c          666 g         2117 t
ORIGIN      
        1 agaataaatc tatatctctt aacaaatgaa ttgtcataca ttgattgttc ttatttagcc
       61 ttagaatcaa tgctcttaca aatggaaatt gtattctaga attaaataag ttagaaccat
      121 cagtatctat atctaagcta cgataattaa tatctataaa tatatctaca tcatcatttt
      181 cttctcttat tattctgtag tcaaaatcca agtttaaatt taaattacct aaaatcaata
      241 acatttaaat cctcttttct ataaatagtt ttagagtgtt aatttaagct ctccatctat
      301 tatagatata attctatctg cttttctagc aatattttca ttatgtgtta ttattataag
      361 ggtttggtta tattttttta tagaactttt tagtaaatcc atgacttctt ctgtagtttt
      421 actgtctaaa tttcctgttg gttcatctgc taggattata cttggttttg cagataaagc
      481 acgtgcaata gcaactcttt gctgttgacc accagataat tggttgggaa gatgattttt
      541 cctttcctta agccccaata aatttataag atcttcaata tattctttat ctattttttt
      601 attgtctaac tttaatggta attctatatt ttcctttaca tttaatacag gaattaggtt
      661 atattgttga aagacaaatc ctaccttttg tcttctatat cttgctaatt ctttatctga
      721 aagacaacat atatctttac catctacctt tatagtacca ttacttggtc tttcaagacc
      781 acctactaaa tgtagaaatg tactttttcc acttccactt ggtccaatta ttgcaacaaa
      841 ttcaccttta tttattttta aatttgcatt ctttagtgca tgtacagatg tttccttttt
      901 cccataaatc ttacctaaat taatagtttc caatataggc ataattatcc ctcctattct
      961 gtgatgttta tagcttcaac tatactaatt ttatttatct ttcttgatgg aatatatgta
     1021 gctagaatac ctacaattat atttgctaca acaactagta tatatatttt gtaatctatt
     1081 gaaaatccca atccatgtga tacaaagccc tgcatataat acattttaag ttgtattata
     1141 agtcctgaca cgatagtaat tatacttgag agtgcaccat ataatattcc ttcataaagt
     1201 atcatatttt taaattctct ctcagagata ccaatagctc ttaacattcc aaattcacta
     1261 gttcttgaag ttaagttata acttatgttg tttattatgt taagtacact gattatgaac
     1321 attattgcta ctatgccata actataaatc atagttctag atgtcatctt atcagaattt
     1381 tctttttcta ccatcatatc tactgttgta gttccaggaa cttcacttcc aattttacct
     1441 aaaaggttat ttattttttt cttgtttgca ttttcattaa ggtctgcata aattaagtta
     1501 tatttatcta caccagttaa tttttttagg tattcgctac tcgttattac atctacacca
     1561 tcatctccag agtacatacc attatctgca aaagtataac ttataatagc tccaacttta
     1621 aattcatatt ctaagtattc ataattatct tttagacttt cataaagccc tgtatccatc
     1681 ccaccctttg gatatttgac ttttatagta tcacctactt ttatatctac agctggtgtc
     1741 ccttcaccag gagtaaaagt ctttgtgtat ttctgtgttt tagaaatttc tggtacatat
     1801 acaatagcta aattttcttt tttcattttt ttcaagttta tatttccact tactaagtat
     1861 ttttctaatg actttatcat ctcatcatta taacctttta gcttttgctt tagtgtatat
     1921 ttgccagtag tcttattttt tatgaacaga ggatttagtt tataataagg ttttgaatta
     1981 atagcttcgt agtaatctaa tttatttatt ttatttacct ctattcttgt gtttaagata
     2041 ctagcagttt tgactttgtc aattccatct atatttttta ttttatctat ctgactatca
     2101 ctaaaccctt ttatatcttt aaaagaagta ttgttaccag tttcattgag ataaacattt
     2161 gatagaataa aatctgcatt catatatgtt tgttcttgca cggatttgtt ttgcacatct
     2221 aaaaaagaat atttataatt gtcttttatg acgattatac ctcctatact catagataat
     2281 acaattatca tgaacccctt tttatttcta aagatgtttt tcgcagatat agattttgtt
     2341 actgacatat ttaaagttaa ttttgatatt aaataattaa ctttggttac atttttatca
     2401 gctccaaaag tttttctgat agcatcaata ggagaaattc ttcttatttt taagtatgta
     2461 aagaaactta taattagtat tattagtatt gtacatgcaa ttgataagag aattatttta
     2521 tctggaataa caaaaggtgt aacatttata tttccttcaa attgaatatt tcctacagac
     2581 ctattaaata tttgtgcacc taccatacct atacatatac ctataggcat tgctattaga
     2641 gcaagtatga aaagttcata aagcataagt ttgaaaatcc taaagttggt ggaaccaata
     2701 gctcttaaga cgccatattc ctgtactctt tggtatattg atataacata gatactatat
     2761 attacaatac cagcaaatag actcatagca attgcaacgt atttacttga attatctaaa
     2821 gtaccgtttt tcatagtaga ttctataagc attgagtttc ttcctatact tttctcatct
     2881 aacatagcat tttttattat attgtctatg tttgtattta tatcactgcc ttcattaaat
     2941 tcaatataag ttgtaaaatc acttagtttt gattcattga gattagaaaa aatttcacat
     3001 tgtcccattt ttttctctat aaatctatct tcaaggattc caacaacttt aaaagtttct
     3061 ggattttctt tttgatataa tttaaaagta aggttttgat ttatctctgg ttttaagccc
     3121 atactattta aaatccattt ctcgactacc acctcattgc tagcttttgg aaatcgacct
     3181 tttataagtt tagaatgact agtcaaataa tttttatttg catatagtat gtttataggt
     3241 aatttttcat taacatctgt agacgcatag taagatgtta tacccagatt tttaatatct
     3301 ttgctagatt ttatggactt taattgattt ttctttatat ttttaaagta tacatggtca
     3361 gttccaagct ctcttttcaa tctatctaca tctgcttgct gagcacttct tgagagggta
     3421 ccaataccaa caacgagtga tgtagcaaga attatactca caacaattgc taaagaccta
     3481 gctttataac tttttatata ccttagtact attttcaaat tatctttcat atttaccact
     3541 tcctttctta actaaatcaa ttatatattt atctaaaata tatagcaaag taagtatttt
     3601 caagttttaa gtagttaatt gtacaaattc atctgtattt ttacaagttt aattaatcta
     3661 tatgtaaatt ttaacttttt acataatttt tttaaaatga tataatccct atataaattg
     3721 tagaggggag ggaaatcatg gctaacaatt ttgtatttcc tatagtaata ctaactttgt
     3781 ttatatttag caatcttata atgaataagt ataaatttta caaagaatta gacatattaa
     3841 aaaaaggcat attaacattt tttatatcgt gttttgcatt gattttattt atggagggca
     3901 ctaaatatat acaattaagg ttaggatata gttttttact tgaaggagta ttaaatatta
     3961 tacttttttt ggttttaatt attttaagtt tttatacagg ctttaaagtt ataagattga
     4021 taaataataa taatgaacag gaatataaac tacaaaaact agatagtaaa ataaatcata
     4081 aggataatat aatcatcaaa ttaagaagtc aaaaacatga ctatttgaaa catttgcaag
     4141 tggtgtattc tctattaaat actgggctag atgaagatgc caaagaatat ataatgaata
     4201 tatcaaatag ttttaaatca aaaaattcaa ggtatggaaa agtatcttat ttagatgcta
     4261 tagcttcatt aaaatacgag gaatgtcttg aaaaggatat atcttttgat gtctacattc
     4321 aagattttat ggataattta gagattgaac ctaatgatat gagttcagta ctattaaaca
     4381 taatagataa tgcaatagag tctttgtcta ctataaaaaa agaaaagaag tatataagat
     4441 tacatgcata tgaagatgaa tttcagtata ttatagctat aaaaaataat ggtcctaaaa
     4501 tacataatgt tgaaagtata ttttggaatg gatttagtac taaagaagga caaaataggg
     4561 gatatgggct acatttagtt aaaaatatat tagataagta tgattatagt atacttgtaa
     4621 atagtgatga ttttttaact gaatttgtaa tcttaatccc aaaatattat tagttaggag
     4681 gagaaaagtc tatgataaaa gttttgattt gtgatgatga taagattgta agagatgata
     4741 tagcaaatat agctaaagag tctggatatg tagattatgt taaaaaagtt aaggatggta
     4801 tagaggcaat tgaatttata aaaaatgaga aaatagattt attgattatt gacattgata
     4861 tgccatataa aaatgggatt gattctgctg aagaaattct ttctatagat aaggatatac
     4921 atatagtatt tgtgacgggt ttcgctgatt atagtcttga aagctttaaa gtgcatccta
     4981 tagattttgt agttaaacca tttaaaaaag aaaaaatact tgattcaatt aacatagcaa
     5041 tagaccatat taactcccat aagattgcta ataataattt tatagaagac accctttttg
     5101 tatataaaat aaggaagcag atacatatga ttaattttga tgatatagtt atgtttgaga
     5161 aaaattcaag agcaattaat ctacacacta aagatgaaga tataataaag ttttatgaga
     5221 actttgatga tttaaaaaaa cgcctacctc ctaatttctt tatgacccat agacaataca
     5281 tagtaaatct aagatttata cataaggtta tacctataaa taaatcttca ttagagataa
     5341 ggtt
//
LOCUS       DAGSHT010000219         5317 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGSHT010000219 DAGSHT010000000
VERSION     DAGSHT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.225"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(931..1593)
                     /locus_tag="MJB72_003240"
     CDS             complement(931..1593)
                     /locus_tag="MJB72_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264303.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(1610..4165)
                     /locus_tag="MJB72_003241"
     CDS             complement(1610..4165)
                     /locus_tag="MJB72_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8264304.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNRLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(4398..4592)
                     /locus_tag="MJB72_003242"
     CDS             complement(4398..4592)
                     /locus_tag="MJB72_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBY8264305.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(4604..4783)
                     /locus_tag="MJB72_003243"
     CDS             complement(4604..4783)
                     /locus_tag="MJB72_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264306.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(4788..5045)
                     /locus_tag="MJB72_003244"
     CDS             complement(4788..5045)
                     /locus_tag="MJB72_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264307.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
BASE COUNT         1746 a          861 c          553 g         2157 t
ORIGIN      
        1 atgctcttac tatagttcat gctaaattaa tttcaaaaaa gagacctata agtaggtttg
       61 tgtaaatgca gacttatcta cagtcttttt tgaacgtaaa attggtaagt atagaataat
      121 attcatatta aacttttgta ggcaaagaaa tgaaaaatca aagagaaata attattacag
      181 atttagatta ccaagctgaa ttaagaaagt gtaaaactat ggaggatgta gttagtgaag
      241 ataatatata atagtttaac ttataaatta tcttggtgat attaatgaag tttcaaagta
      301 tatattattt attgatataa ataaaattaa atctgttgct aatagatgta cctttgtttg
      361 gagacaaact attgaagaaa tataactaaa atgttgaagt agctacctga ctttattttg
      421 aaatgcgaac aagattttgg cattaagata atttagaaag aatcactggt acacaaggat
      481 gtgaacttag aatgaataga agtattcagg cataaggagc tttcacccaa ataaaacata
      541 gtatgaactt taaaagaatt ctaatcagag gcaatcaaga tattctttca gagtttatac
      601 ctgtagtgtt agcctatagt atttctaagt ttcacaaata agataatcat taataaatgt
      661 agtatgtata ttcatcctct caaaaatatt ttttaatctt acactttgta aattattcta
      721 ttttaaaatg gtatttttat acctttattt cttatgaccg ttttcaagct caaattttgc
      781 ataaatataa attaatgaaa agcaagtaaa aaaagaactg tcgcacagca atttaaaatt
      841 actcattcga cagtccttat ttcgtggatt taaatagtgg agaagaatca caagaataat
      901 acccacgatt aaacctttat acaatacatt ttatatgact ttaccatcac taatagttat
      961 tatacaatca gccatcttag ctatttcatc attatgagtt atcataacca acgtctgctt
     1021 gtattcattt acacaatact ttagtaaatt catcacctca ttagttgttt tactgtctaa
     1081 atttccagta ggttcatctg caaaaattat tgatggctta ttggcaagtg atcttgctat
     1141 tgcaactctt tgttgttgac cacctgataa ttcattagga aatttattaa tctgactttc
     1201 taatcctaac ttagttatta aatcattaat ataattatta tctatctttc ctttagcaat
     1261 taaaatagga agaactatat tatcataaac attgataaca ggtattagat tatagttttg
     1321 gaatataaac ccaaattctt tacttctaag attagatagc ttttcatcac ttaaagtata
     1381 taaatctaaa ttatccataa atacattacc taaagttggt ttatcaagcc ctgccataca
     1441 gtgaagtaat gtgctttttc cagaaccact ttgtccaatt attgcactaa atttatgggg
     1501 ttctatctct aaagtaattc catcaacagc ctttatttca ttttctcctt ttccatatat
     1561 ttttttaata tctataactt ttaacatctt catacattta cctcccaaat tattcattct
     1621 cttttatacc ttcaataata ttcatacttt caaaatctct attacttaca actactgcta
     1681 ttaaacatac aaatacagat atagcaaaga atagtaaaga ttccttaatt gggaatgtaa
     1741 aagacataaa attctctatt ccatagttta tatacgcatc attattccaa tttgtaaatt
     1801 taactagatt tataaatgat ggaatcatag ctattataaa gctaaaaact gcatatgcta
     1861 atgcttcata tataagcatt tttttcatat ttttaagact aacacctaat gctctaagcg
     1921 ttgaaatttc tttttttcta attagtagat ttgttcttat agtacagaaa atattaattg
     1981 atgctattat taaaactagt gcaattattg ctatttgtga tttaatgtaa cttttatata
     2041 tactttctga catttcatga taacttaatc tactattaaa tagagaccct ggtatacttc
     2101 ttgaaatttt ctgtatctca ttattaacaa tctcatcata tggattttct aaatttatgc
     2161 ctaattgagt gtatttttgt tctcctgtta tgtttttaga gtgaatatta cttgttataa
     2221 tttctagatc tggcataaaa tcgccatctc ccatagcaat ccattcatcc tttaaaatta
     2281 cagccactct taccttcatc tctttataaa caattgtatt attttcttca acaggaattt
     2341 ttacagttat tatgtctcct atatttaatt ctttgaatat aggctttata gtatgatcat
     2401 caactatatc ataaaaataa ttatatactg ctacatttgg atatccatca gtgtcctttt
     2461 taatatcatc taagctacct tcttcaacaa agtcatttaa cttttcatct atataatcat
     2521 catcataaag ctttaaaact aatttatctt ccattatctc ttttttttct actccattat
     2581 aggttgtaaa ttctttgttt atatccttat tattaaaagt aaacattcca tttgttattt
     2641 gaattttatg tatactttta acacccttaa ttttttctat attctctaca tcactatccg
     2701 tgtattttga tatttgcggg tctgcattta ctccaaattg cattgttata ttatatctat
     2761 tttgcatatc ttcaatttta ctgttgacct cttcttgcat acttgagaat gtagtaatga
     2821 atatatatcc tcctaaagca atagacaaca tagatatagc agctctaacc ttatttcttc
     2881 caatattttt aagtcccata aatccataaa atccaaatat tttgcttact agtctgtcta
     2941 ttccaagtct acttctgtga tttttaattt tgtctgtatt tattattgat tcaataggtg
     3001 atatcttgcc gattttaaaa gttggtataa tacaagatat aaaaacagaa attattccaa
     3061 ccaatatagc ttttataact atttcattat ttatatataa tttagggtta agaccagctt
     3121 cactgtataa aacttcgtta taattactaa ttcctatata tgaatatgta accccagcta
     3181 ctaacccaag tagtatacct attattgtta taattaatgc ttgatagatt atcatacacc
     3241 ttacgtttct tttctttgca cccatcagtc taaacagtcc catttctttt actgattgat
     3301 ttagtgtaat attgaaaata ttaaatatga aaattataga tactgtagtt attaaaacat
     3361 ttcttttaaa aacattcgga tcattcttat catcttctac ataacgaagt tcccttacaa
     3421 gaggtacatt tggtagaaaa cttttttgtc ctaacttata ttttttcata agataatcac
     3481 attcaccttc aatatttctc catccacttt taaaacttaa tatactatta taactaatcg
     3541 catcatgagg tataatgtta tttgtttcat cattgccata agttactgct tcgtattgcc
     3601 aaaccacatc atagtaccct tttggttttt ccataactcc tactatttta aattctttat
     3661 ttatagaaaa taatcgatta cttccgttag tatctttata gttctttttt attgttaaat
     3721 ttaatgtaga tcctactcct tcacttaaat tcattgcttc tagtgctttt ttctctaata
     3781 ctatttcatt gttgttttta ggtagtctac cttctattat tttgttgtat ctacttttta
     3841 gatactcttc gtttgatgaa gttaatataa atgagtttcc ttttttatct gaaatacgtc
     3901 ctaaattttg taccgaatta ccctttgata ctttttcatc agtttctaat tttttagcat
     3961 catcaagtgt caaatcacta aactctacat ggtatccact gtaaagttca tgtattgcta
     4021 ctacattgtt ttcattttgt gaatctttta taacactagg tccaaatatc aacataacac
     4081 ctaacataac tcctattatt aaaagtagtg ttctaagttt atttttcttt aaatatgcta
     4141 gtgacatttt taagtaaatc cccatataat cctcctattt tactataaat tatttatcca
     4201 actcatctgt taagtagatt ggtggatatt ttaaatcttt taattcttca ttattttttg
     4261 aagcttacta ccatcttcct catgataaac ttgcagtatc accttattta catgtagtgc
     4321 ataataatca ccactataaa aatattggta ttcataacct ggataatttt tgtaaaatga
     4381 tttataaagt acccatccta attctctttt gtatactttt cttttgtaat acgctctata
     4441 tcatcacctg aaactccttg atatgtcata tatttatcta tatatttttc agcttcattc
     4501 cattttctaa aatagttgtt gtatataata aatcctatta tggctataat gcttataatt
     4561 aaagttaata aaatagcttt gtattttctc atagtgaatc ttactacttc aatttaaatt
     4621 ctttaataag ttttaattct aaagacttag ttttatcatc aaatttctta acttgaattt
     4681 ttatatctcc caaattatca acctctatat ttccttcacc tttatcaaat acttcttcaa
     4741 tttttctaaa aaattctttc tcacttattt ctacatgaat caatgatcta tatgttataa
     4801 atagccatta tgaatttatc atcttctgac agtttttcat tataagaaaa atctttaaca
     4861 cattgaattg ataatatttc ctttgtcccc ttcttgtcct tagtcattga ataatgtaaa
     4921 acttggctag gttgttcttt tgtatactcc aagtttccca aagacaaaac aaaacttcca
     4981 tctgatttat tttcagctac cctaagacca ctattttcta ttgattctga tattttcttt
     5041 gacaagtctt cttgatacta gaaatcggtt caatagtata tattttttca tttgtatctg
     5101 aaaccttatt tgtgcatcct gttattaaac ctagcgttaa gcttatagct ataaccatag
     5161 ttattgtaat ttttttatct tcatataatc ccccttatat attgaaaact cgttatttct
     5221 cataaaaaaa agacgctatc tttttctatt aaaagataac atccctatat gtttagtatc
     5281 aatatatgtt ccccaactgg taggttaatc gacccaa
//
LOCUS       DAGSHT010000220         5292 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.226, whole genome shotgun sequence.
ACCESSION   DAGSHT010000220 DAGSHT010000000
VERSION     DAGSHT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.226"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            224..1303
                     /locus_tag="MJB72_003245"
     CDS             224..1303
                     /locus_tag="MJB72_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY8264308.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKYTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1627..1995
                     /locus_tag="MJB72_003246"
     CDS             1627..1995
                     /locus_tag="MJB72_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBY8264309.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2112..2915
                     /locus_tag="MJB72_003247"
     CDS             2112..2915
                     /locus_tag="MJB72_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8264310.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="MJB72_003248"
     CDS             3307..4197
                     /locus_tag="MJB72_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8264311.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
     gene            4760..5203
                     /locus_tag="MJB72_003249"
     CDS             4760..5203
                     /locus_tag="MJB72_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264312.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         2022 a          549 c          971 g         1750 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atagtttaac agtaggaaat atcgtatgag
      121 taagactatc gtattttaca taaagattta gcaatatgac acactgttgt cgtgttctgt
      181 aggatatgat taataaatca gatatgaaag gagagtgagt aatatgccag aattactaaa
      241 aaatatgtat aatcgtgagt cactttatga agttgcagta gctatccaat cagtttataa
      301 ctcatttaag gtagacgagt tcataaaata tacaatggat gagacatgga ataacttaga
      361 attaaaagcc agatgtcgaa agataagtat gagtttagga atgtatttac cagaagacta
      421 taaggaagct ctttccattc ttgaaaaatc agttacaggt ttttattttg cttttttctt
      481 tccagatttt gttgaagttt atgggcagga cgacataaat tgggatttat ctataagtgc
      541 actagaaaga aatactgagt attggtcttc agaatttgct gtaagagcat ttatcatcaa
      601 ggatgaagag cgtatgatgg cacagatgcg taaatggtct aagcataaga gcgaacatgt
      661 acgaagactt gcaagtgaag gctgtcgtcc ccagttgcct tggggacaag caatatccaa
      721 atttaaaaaa gaccctacac ctgtactgcc tatattggag caacttaaaa cagatacatc
      781 aacgtatgta caaaagagtg ttgctaataa tctaaatgat atctcaaaaa cacatccaga
      841 tttagtcata agcattgcaa aggattggta cggtaaaaat aaatccacta actggattgt
      901 aaagcatggt tgtagaactc tactaaaaaa aggcaatcgt gatgtattag ctctttttgg
      961 atatgatgat actacttcta taaatcttca ggactttact ttagaaacaa cgtctatttc
     1021 tattggagag gatttaactt tttcatttaa tattttagca aaaaaagcga caaaaacaag
     1081 gctcgagtac ggaattgatt atattaaatc taatggaaaa aggaatagga agatatttaa
     1141 aatatcggag gtttcattaa aagaaaatga gaaaaagtca tatatgaaaa aacattcatt
     1201 tgcagatgta agtgtaagga aacattatcc aggtatacac tcgattgcta ttattattaa
     1261 tggtatagaa aaagataaac ttgattttga attaggtata taattttact aacagaataa
     1321 tctatatcag atactttgct aaacttatat aatatatttt actaaattta tattaggtag
     1381 tttactactt gtttactgaa tttaaataca aagaaataca ggtgctaatc cctgcccatt
     1441 aaaggaatat gagcataaca caaaagtaga acttatcaat tctgttacgg gtatagtagt
     1501 aaatccatag aatcacaatt agaagatgaa gtgtaaaaaa taagttagtg ttgattttag
     1561 ttgctagtga tattgaatag ttatacaata gccatataaa ttagaatttg tagaggagaa
     1621 atataaatga atgtatatag tttttggaat gacactttgg cacaagatgc tgttaagata
     1681 agaaaatatt ttcatgaaga tgcttatatt aattggcatg atacaaatga gcattttact
     1741 ttagaagaat ttattattgc aaattgtgag tatcctgata aatggaatgg taaagtagaa
     1801 agggtgctag aatttggaaa tttagtaatt acagtgacac atgtttatgc aataaataaa
     1861 tcactatcat ttcatgtcgt ttcatttatc agccttaaag atgataagat tatttctatt
     1921 gatgaatatt ggggagagga tgatgatgtg ccacagtgga gattagataa acatattgga
     1981 aagccaataa aatagtgttt gttctgtttt ataactgaaa ttttgataac atttgatatt
     2041 ttgatttgtc attggtcaac atagtcgtta gtgtaataat ttaaaaaagt agaatttgga
     2101 gagtgaacac tatggaagaa aaatacatct tatctgaccg agggaaagta tattattgga
     2161 taaaagaaag taatagtgtg tttgcaaaga acttaatttt tttgccagga ctaacagcaa
     2221 atcatcatct ttttgatttc cagatacagt acttttcaaa aaatcataat gtattggtat
     2281 gggacgctcc tgctcatgga aaatcaagac cttacttaga tttctcatat tctaacttgg
     2341 tagaagaatt gaaagtaatt ttagatagac aaaatatgaa aaaggttgta cttatagggc
     2401 aatcggcagg gggatttgtg gcacagtcct ttattgtaaa atatccaaat atggtagaag
     2461 gactaatgat gattggaact tgtccatatg gaactgacta ttatagtaag tcagatattt
     2521 tctggttaaa acagactgaa tggatgttaa agttattccc agataaattg ttgcgaaaca
     2581 taatatctaa gatgtgtgga gctacgtctg aaggcagaag gagtatgctg aaaatgttag
     2641 aaggttacga aaaaaaagaa ctgtgtcatc tcatgtatat gggatttgct gggttcatac
     2701 cagaaattca agacttaaat atacattgtc cagtttgttt ggtggttgga gataaagaca
     2761 agacgggtaa agtgagaaaa tataatgagc agtggcacaa taagactggg tatccattgc
     2821 acattataaa aaatgcttca cataatgtaa atgtcgataa acctaatgaa ctaaatcaga
     2881 ttatggatat atttattaag caattaaata tataaaaact acatactcat taggtaaatt
     2941 atgtagtata tgtgtttaca gatggttata gaaataaata tgtaataaac tacttaacac
     3001 ttaaaaagtg gatttgtcca gagcataata caaagcatga tatatataaa tttttaagcg
     3061 aaatacatca aaagaaggat tagaattagt ggagacagaa cagctattat agattataaa
     3121 taaatagttt taaaggagag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 agtattataa atgattagtt taaaagtact atcggatagg agtctccata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggtgta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaagcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgacgcagtg aaagaactta atgattctgg aattttttgt
     3841 ggcatattat tgactcctat gcttccattt ttgactgata ataaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgcatgta tggtgtaaca
     3961 atgcgtagtg gtcagagaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtacttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tcttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtgttgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtattg atattttagg taattgttag agctgatatt
     4321 atagatgaat attagtattg atattctagg taattgctag agttagtatt atagatgaat
     4381 attagtattg atactctagg taattgctag agttagtatt atagatgaat attagtattg
     4441 atattctagg taattgctag agctgatatt ataatttatt attagtattg atattctagg
     4501 taattgctag agctgatatt ataatttatt attagtattg atattctagg taattgctag
     4561 agttagtatt atagatgaat attagtattg atattctagg taattgctag agctgatatt
     4621 ataatttatt attagtatta atattctagg gaattatcac tatattttaa aaagtaaaaa
     4681 tatatatcaa aatgaattaa ataagtactg taagaagttt gttaaattaa ggtttgaata
     4741 aagtgagggt ggagaactta tgatagaaat aaaaactata gaaataaata aaaaaagtcg
     4801 aattgacctt ttaatgttag ccgacccatg tgaagaaatg attgataaat atctcaataa
     4861 aggaactatg tacgcattgt atgataataa agaacttact tgtatagcag ttgtaaatga
     4921 aatttccaaa gaaatatgtg aattaaaaaa cattgctaca tatgagcatt ttcaaaatat
     4981 gggttatgcc tcaaagatga tttatcatct tttagatgtt tatagtaaaa aatattcatc
     5041 aatggttgtt ggtacatctg aaagcggagt tcctttttat gagaagtttg gatttgttta
     5101 ttcacataaa attaaaaact tttttgtaga taattatcca gaaccaatct ttgaaggaga
     5161 attacaatgt gttgacatgt tgtatctatc atataacttt tagataggat aatattttta
     5221 ggtagatata attaattaat tcatataatt atcggaatta taatttataa attatgtata
     5281 ataagagtat aa
//
LOCUS       DAGSHT010000221         5252 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGSHT010000221 DAGSHT010000000
VERSION     DAGSHT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.227"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            29..760
                     /locus_tag="MJB72_003250"
     CDS             29..760
                     /locus_tag="MJB72_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBY8264313.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTE
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            840..1475
                     /locus_tag="MJB72_003251"
     CDS             840..1475
                     /locus_tag="MJB72_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBY8264314.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNKVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1725..2318)
                     /locus_tag="MJB72_003252"
     CDS             complement(1725..2318)
                     /locus_tag="MJB72_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBY8264315.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2389..3252)
                     /locus_tag="MJB72_003253"
     CDS             complement(2389..3252)
                     /locus_tag="MJB72_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBY8264316.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMVANVGKWDKFTYKSLVNALEFKDELYLENPKEEKTYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            3415..4584
                     /locus_tag="MJB72_003254"
     CDS             3415..4584
                     /locus_tag="MJB72_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8264317.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSSLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            4685..5161
                     /locus_tag="MJB72_003255"
     CDS             4685..5161
                     /locus_tag="MJB72_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY8264318.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         1882 a          775 c          668 g         1927 t
ORIGIN      
        1 gttatactta taggagggaa ccatataaat gaatatttat gctcataggg ggtttagtgg
       61 aaagtatcca gaaaatacta ttctagcttt taaaaaatgt ttggatatgg atatatatgg
      121 aatagaactt gatgtccaca gaactaagga tggaaaaatt gtagttattc atgatgaaaa
      181 ggttgataga acttttaatg gacatggttt tgtaaaagac tttactttaa gaaaattaaa
      241 aactctaaac tcatcttttg aaggatatca aagcaataaa gaatgtaaaa ttccaacttt
      301 agaagaagta ttaattctaa tttcacctac agaacttatt ttaaatatag aactaaaaac
      361 tgacaaaata aactatccca atatagaaaa agacgtttta gagcttattt taaagtataa
      421 tatgaaaaat agagttctta tatctagttt caacagcaat agcttaaaaa actttcataa
      481 attagaccct agtataaaaa caggattatt gtgttattta cctataaaca atgtagtcaa
      541 ttttgctaaa tttctgggta atagttacct tcatccacct ctagttttag taaatgaatc
      601 tctaattgaa ctttgtcaca aaaatctact tggtgtcaat gtatatactg taaatgaaga
      661 ggatgatata ctacactgcc taaaactcaa tgtagatggt atttttacta actatccaga
      721 tattgcttca aatcttttac actcaaaaca atacagctaa ttatacaata gtttgtactc
      781 cttgactttt atataattaa tatgtacaat tttattaaat ttatcttgga ggtataacta
      841 tggataactt attaaatgaa cttgaagctt taaagagtaa tttagataat ctaccaatag
      901 aaaaaataga aaattatgat ttgagtaaaa ctgctttatt tataattgat gttaataatg
      961 gctttgcaag acaaggtgca ttatactctc ctagagtaga gtctttaata aagccaattg
     1021 aaatgtttac aaaaaaaata tcaaacaaat taaataaagt aatagcattt actgattctc
     1081 atactcctaa atcaatagaa cttttgagtt atcctgttca ctgtttggaa aatgatgtag
     1141 aaagtgaatt ggttgatgaa cttaagtcga tagaaaacct acaaattctt cctaaaaatt
     1201 ctactaatgg attttttgca ttagaaaatt tggattttga taatatagat aatataatta
     1261 tagttggcga ttgcacagat atatgtatat atcaatttgc aataactcta aaatcatatt
     1321 ttaatcaaca taatattgaa aaaaatatag ttgtacctat gaatttagta gatacttatg
     1381 atattcctaa cgttcaccct gctgagattt taaacttagt atttttcaac agtatgattc
     1441 aaaatggagt taatgtatta aaagagattc gttaagcctc taatacttta catccatata
     1501 atacaattat cagatattta tttttaataa aatttctaat agaaaaacac agctttagtg
     1561 agaagattac aatttaaaac actatctatt atgactaatc ttcacaatat aaaaaatcgc
     1621 agtttagata ttccttatac aaatttataa ggaataataa attgcgattt ttatttttaa
     1681 gtagtacaat taattgatac tacttcagta tttaaattca ttttttaata atcatactta
     1741 ttcgtagttt cgcacattac tacctttatt tttttattaa acatattctt atattctaat
     1801 aaaaactcat ttagttctga atccataaga tttatttttc tatcactaca aagcattatc
     1861 ttttcttggt cgattattac attaatatca tttaagtcat tttttataaa tgttgttata
     1921 caactctttg gttggatttt ttctccatat ttctcataaa atcctgcact cttcttaaat
     1981 gactctaaat cttctgtaga tactttgctg gaagtattat tactcataaa acctgctgta
     2041 gaagggtcga ttccaaagtt tctaagttgt ttttctactt cttcagtgtc cataccatat
     2101 ctctccataa attgcttttg tattttcata aatttttctt gagatatgtt gttatctctc
     2161 atataatcga atatttttct tgaaaactca gccatagaca tctttccatc cataacagaa
     2221 aaatataagt tatatatgta ttgctcaaat ttatctacat cagaatcttt aactttgttc
     2281 tttagttcgt taaagtctat tattctatca tcagacattt ttcatcctcc tagctaaatt
     2341 tttacttgta taagattata ccatatttct attatattta tatacacact atttttttaa
     2401 tttaacgatt ttttctgtag catctaattc gcacattctg cccaaaggaa tagttatcat
     2461 tgggatacaa tgtcctgact gaagattata tatagaaggt ttattgacct tatttgcaaa
     2521 ttctattagt aattccatca aatcattttc ttcaatacaa tccttaaaat ctccaaaaat
     2581 tataccatta cattcctcaa gttttcctga agaatatagt tgtgttaaca ttctatccaa
     2641 tttatattga ggttctccaa tttcctcaat aaacaaaatt ttatcttttg catctatttc
     2701 atattttgtt cccattgtgg caattattaa ggataaattt cctcccatta ttattccctc
     2761 tgctttacct tcacatacag tataagtctt ttcttctttt ggattttcta aatataattc
     2821 atctttaaat tctaaagcat ttaccaatga tttataggta aatttatccc atttacctac
     2881 atttgctacc attattccat gatatggtaa aagaccacaa tttttataga aagctaaatt
     2941 taaacctgtt atatcagaaa aacctataaa tggttttgga ttttgcttta ttatatcata
     3001 atttatttta tccaatattc ttgttgttcc ataacctcct cttaggcaca taattatatc
     3061 tacatcttta tctaaaaaca tatcttctat atcccttgct ctaatatcat cattaccagc
     3121 taaataacct ttataattta aactacaact ttcagaaaac ttaacttcgt aaccataact
     3181 ttctaaatta aactttattt cctccaaact tgtatttctt aatggcccag atggcgcaat
     3241 tattcctaac atttaaacac ctcttcttat aaaatgatat attcttctcc atactaaata
     3301 tctctaatat aattataaga tattttattt gtacttgata cataataatg atttacataa
     3361 aataatataa ttaatgaaga aaaataaatt tttaatatag ttaggatgtg ttttatgctt
     3421 aatataaaaa ctctttgtca tgagataaat gattgggtta taaatataag aagagattta
     3481 cataagactc cagaacttgg attagaagaa tttcaaacta agaaaaaaat tattaaatac
     3541 ttaaatgaaa taggtataaa ttatattgaa tataagaatc atacagggat aactgcatac
     3601 attaacgtga gtccaaactt tgaaacagtt gctataagag ctgatataga tgccttacct
     3661 ataacagaag aacttaatta ttcttataaa tctattaata ttggcaaaat gcatgcatgt
     3721 ggtcatgatg cacacactgc tattttactg ggtacttgta atatactatt taaattaaag
     3781 gattacttaa atgtaaatgt aaaattcttt tttcaacctg ccgaggaaac tattggtggg
     3841 gctcaactta tgatagaaga tggatgtctt gaaaacccaa atgtaaaata tatatttgga
     3901 cttcatgtaa atcccaatat caacaaaaat ctaattgaat taaaatacaa cactctaaat
     3961 gcttccaccg atacacttca attaactgta catggttcaa aatgtcatgg agcttatcca
     4021 catcaaggta ttgatgctat tgttatatct gctcatatta ttacagctct acagacaatc
     4081 gttagtcgaa acacaaatcc aactgattct gtggttattt ctctaggaaa aattgaaggt
     4141 ggaatcaagg aaaatatagt ttgtgacaaa gtggttataa ggggtacttt gagaaccttg
     4201 actcctgaaa ctagagagtt ttcaaaaaaa cgaattagag aaatttgcga ttttacttgt
     4261 aaaacatttg gaggaagtat ttctgttgaa attgaggaag gttatccagc tcttatcaat
     4321 tcaaaccatc tagtagatta cgtcaaacaa aatgctgtag aattgtttgg tgaagaaaat
     4381 attatcctta aagactcgcc cactcttggg gctgaagact tttcgtattt tctaagacac
     4441 tgtgaaggag ctttttacca ccttggttgt gcaaatcgtg aaaaaaacat tacttcctct
     4501 ttgcatactt ctacttttga tattgacgaa gattgcctta ttactggtgt catgctccat
     4561 gtaaaaaatg tattatcatt ctaactatta atatttttat ttattatttt atattgaccc
     4621 tcacatgatg tcatgttata ccctaaaatt acaaatgata aattaaatag ttggaggtaa
     4681 tattatgaaa gaaatattaa taaatcctat aggtaaaata tgtattcaag gcgaagaggt
     4741 ctttattaaa ttagacaaaa agtatgtacc tgcattaaat gaactcgacg atttcagtca
     4801 tttaaatgta ttttggtggg ctgatgggtt tgataatcct gaactaagaa gtattttaga
     4861 aaccccaaaa ccttataaaa gtggaccaga cgttatagga atatttgcaa caaggtcacc
     4921 tataagaccc aatccaatag cattgactgc tgtacaaatt atcaatatcg accatgaaaa
     4981 tggcataata aaaataccat atattgatgc taatgataat agtcctgtta ttgacttaaa
     5041 gccatatact ccaagcgttg atagggttga aaatccgtcc gttcctaaat ggtgtagtca
     5101 ctggcctaag agtgtagaaa aatcaggaga atttaattgg gaagatgaaa ttgaatttta
     5161 aattactttt ttcttgattt cattaacttg ttttccacat ttattttatt tacacttacc
     5221 aagattttat gtcacatata catataatat ta
//
LOCUS       DAGSHT010000222         5241 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.228, whole genome shotgun sequence.
ACCESSION   DAGSHT010000222 DAGSHT010000000
VERSION     DAGSHT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.228"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            124..1650
                     /locus_tag="MJB72_003256"
     CDS             124..1650
                     /locus_tag="MJB72_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY8264319.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTSYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1678..2235
                     /locus_tag="MJB72_003257"
     CDS             1678..2235
                     /locus_tag="MJB72_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264320.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2757..3794
                     /locus_tag="MJB72_003258"
     CDS             2757..3794
                     /locus_tag="MJB72_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264321.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            3905..5047
                     /locus_tag="MJB72_003259"
     CDS             3905..5047
                     /locus_tag="MJB72_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY8264322.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2215 a          433 c          927 g         1666 t
ORIGIN      
        1 attaatataa ccaagcgatt tttaatttag ctttttaata agcttatttt ttatactatt
       61 tataataaat aaactccatg atgggaaaac tattactatt taatatgtaa aggagtattt
      121 taaatgaatg aatcatattg gtttttaaac tcaagtccaa aagaatataa caaattaggt
      181 gaaaatataa aaacagattg tcttatagta ggtggtggaa taacgggatt gacaacttca
      241 tatttattag caaaagaagg taaaaaagta gtgcttgttg aggctgataa aataggatat
      301 ggaacttctg gaaggaatac tggtaaggta acttgtcagc atgatatatt ttactctaag
      361 atagagaaaa aatatggatt agataaagca aaatcatatt ataatgcaaa taatgaagct
      421 ttaaatttag ttgaacaaat aatagaagaa aacaatataa aatgtgattt taaaagggaa
      481 acttcttttg tatttacaga aaaagaggat actataaagg atattaaaga tgaatatcgt
      541 acatgtaaaa aaataggaat aaattgtgag tatcatgaaa ctatagaaaa tattccatta
      601 gatataaagg gggctattag ttttactaat caaggtcaat ttaacccaaa gaagtatatt
      661 gatggattgg caaaagcagc agtaaattta gggctaaaaa tatatgaaaa tacaccagta
      721 gtagaccttg aaaaaggaaa aatatgtagg gttaaaacaa gagaggataa tataatagaa
      781 gctgagaatg ttataatttc atctcatagt ccatggtatg atggattgaa tttttatttt
      841 gcaaaagaat atgcagagcg tgcttattta atggttgcag ttttggaaaa taaattagca
      901 gatggtatgt ttataagtat agatgaccca tcaataactt ttagacaata taatgatgga
      961 agtgaaaatt tactgatttt tggaggagga gaccataaag ttggtcaggg aggaactgag
     1021 aaagaaatat ttgatgattt agagcattat ggtaaagaag tctttaaggt aaaagatttt
     1081 aaggggaaat ggtctgctca agataatatg agttttgaca atgttcctta tattggttat
     1141 atcaacaaaa gagaagacaa tatttatgta gctacagggt tctctaaatg gggaattact
     1201 aatggaactg ctgctggaat aataataaaa gatttaataa taaataataa ttctgattat
     1261 aaagatactt ttaatccatc cagactaggt agctactttt caaaagattt tataaaggaa
     1321 aatgcaaacg tagcaataaa ttatgtaagt ggaaaattaa aaataggtag tggagatatg
     1381 cctaaaaata atggagaagg aaagattgtt aatattgatg gaaaaagata tggtgtatat
     1441 aaggatgata atggagattt ttatattgta gacactacat gtactcattt aggatgtgaa
     1501 cttaatttta acagtgaaga aaaaacatgg gactgtccat gtcatggttc tagatttgac
     1561 tataagggaa atatacttga aggaccagct cttaagccat taaaacttta tggacatggt
     1621 gataatgatg ttaatcctaa gttattataa aattattttt aggaaggtaa agtgtatatg
     1681 aataaacttt taaagccaat aactatacta ttgtatatag tttatatttt atctgccgta
     1741 ttttttgtag caaacaaaga atatggaagt ctaggtctag tagcaattag tttagttggt
     1801 actttcattt tgagttttat aaataagcaa actgaaagat tattgagtaa agaattatat
     1861 ataatgcttg ctttatttat aatgttttca tcattgttag gtacttgttt taatttttat
     1921 gatataaatt attatgatga ttttttgcat ttatggtcag gtataatatc ttgtattgtg
     1981 gctttttcta taattaaata cttttataca caaagagaga taagtaaaat gagtaaagtg
     2041 tttttagtta tatttttatt tatgtttagt atgggagtag cgagtttatg ggaaataggt
     2101 gaatttttaa tggatacttt tgttgggact actacacagg ccggagggtt agaagatact
     2161 atgattgata tggtagatgc tttaataggc acaattataa ctattccttt tatagtaaaa
     2221 aaatctaata tatagtttta aattttttat attaattata atggatttaa ttaatcatgt
     2281 tgagtttcaa ggtctatatg attaattaaa tccatttttg ttatttataa ttattattga
     2341 atatattttt acatataaga tataaggtga tagatagaaa ttgttggatt atgtaaataa
     2401 ttaatatata ataccattat gggcaataaa tacaaatcaa atgataatat agtctattta
     2461 tgtaaatgtt atattgtatc ctaagtataa tagtaagttt tttgatagtg gtgtagaaag
     2521 taaaaatcta ctagcctaaa ttgatgtatg gattagttta ggaacagtaa ttgcatatct
     2581 taagtttgat gtcatgttgg gctactagaa ataggtcatc tttttcactt gaatatcttt
     2641 tctttaaaca tggggagtat catatcaaaa acatatgaaa attaagtgat ttatgagaac
     2701 agtaatattt tatatgatag tatagaatag taataaagta ttaggaggaa cattgagtgt
     2761 taaaaaagtg gtttggtatt gtgaaaaaaa gacaaaaaag tgagtcagta aaagaagaag
     2821 gtgaagtaat attaaaaaac gaaaaaatat tatctgaaga aaagttgata gatgaagaag
     2881 gagttgtagt taatattgat aatcaaatat taactaaagt agtaaatgat gacaatgaaa
     2941 tagaagaaaa aataatagaa gaagatttgt taattagtga aaataccata aaattagatg
     3001 ataaagaagc aattattaat gataaaaaca tagaattatg taaaaaagaa gttcaagttg
     3061 aaagtgaaaa aataggttta aataagtttg aaggactgga ccaaaatgaa aattataatc
     3121 tagaaaaaaa tgttattgaa gaaaaaaaag taagtgaatg tttgacagaa gaagatttag
     3181 agtacataaa agaaattaaa ataaaaagag gaaaaagtat aaaagctata aacttgtata
     3241 ctaaagaaga gtgggttttt gacactcata tacagtgtag taaaaaactc aaagttccat
     3301 tagggtacat aagagaaaat ttaaaatatg gatatatgga ttactttgga gatgcaataa
     3361 attatttaag tgaagtatta aatatagatg aatactgcaa aagtgaatgg agctatctcg
     3421 ataatagcaa atctccatct gagatattta atattctaaa caataaaata tttagtataa
     3481 ggctttctaa tgaaaaaaga aatgaaattt tgacaaatga taaaattgaa gcattaaaaa
     3541 tgaattatag atttgaatgt attgatgaag aatatgatga atattttaaa aaatataagt
     3601 ctataatcaa aagaggtgga aagaaaaaag ttgaattagt aaataaaaaa ggtgacattt
     3661 tagaaatatt taagtcttta gaagaatgtg ccatttattt gcaaaaggaa aagaatgaag
     3721 ttatacaaat gttaaaatat ggagatacaa aagtaggaag aaactttata aggtatagtt
     3781 tgagaagtat ttaatttcat tttaaattca taagagtttc atttctgatt cacacattta
     3841 atattatttt aatgtaaaat aataaaggtg aagtattgca caattaaaat taaggagggt
     3901 attaatgtct gaagaaaaaa atccaaaaga aaatacatta gatgaatcta aaaattcaga
     3961 taatgtaaat tctaaaaaag atgatgtgtc agatgaaaaa aatactgata acaaagttaa
     4021 aaaaagggaa ataaagctaa acaacaaaac acttaaagct attgtagcaa ttgttgcagt
     4081 aggagctata tgtttaggtg gaggctatgt ttttggcaaa gaagctggaa gaaaattgcc
     4141 tgctacttca aaacactata atagcagtga ggtagtagct actattgggg ataaaaaaat
     4201 aacaggtgaa atgttaagaa agagaatgga gccattattt tatttaaatg caaaaactac
     4261 tatgactgat gacgaaatac gttcatatga atcaaatatg attgaaacta tgattacatc
     4321 tgatatatta tcctctgaag cagcaaaaga gaaaataagt ataactaatg accaactaga
     4381 tgctcagtat aataatttaa tgagtagtat agaatcagca atgggaatga ctaaagaaga
     4441 gtttttaaaa cagtttgatt tgactgaaaa atatataaaa gatgatatga aaaaagagct
     4501 agtagcatca aaatatttaa atgaaaaatc aaaagttact gataaagaag cagagaacta
     4561 ttacaataaa aacaagagca attatttaca agttagagct tcacatattt taataaaaac
     4621 tgttgatgat aaaggtaagc aagttagtag tagcaaaaaa gctgaactta agaaagaagc
     4681 tgaagaaata cttaaaaaag cccaagctgg ggaagatttt gctactctag ctaaaaaata
     4741 ctctgaagat agtagtgcag aatcaggtgg agatttaggt ttctttggta aaggtcagat
     4801 ggtggaatct tttgaaaaag cagcctttgc attgaagaag ggagaggtat ctaacaaact
     4861 tgtagaaagt gattatggat atcatataat taagaaaact gatgaaaaat atcagccatt
     4921 tgaagaggta aaatcagatt tagtaagcag tttaacatca gaaaaacaaa acctacttat
     4981 acaaaatcta aaagaaaaat ataatgttaa aattacagat aaatatcaaa tgaaagctaa
     5041 agaatagata aagtcaattt attaaaaaaa agaaaaatta actgtatgga taaaagctat
     5101 ttttactctc aatatagaga gtataaaata gcttttatta ttttgcaata aaatctattt
     5161 atatatatga agtgataaag aaataacatg caaaaaggta ttaaaaagaa atatttttaa
     5221 tttgaatttt ttcaataaaa a
//
LOCUS       DAGSHT010000223         5241 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.229, whole genome shotgun sequence.
ACCESSION   DAGSHT010000223 DAGSHT010000000
VERSION     DAGSHT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.229"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            834..1064
                     /locus_tag="MJB72_003260"
     CDS             834..1064
                     /locus_tag="MJB72_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8264323.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            1057..1638
                     /locus_tag="MJB72_003261"
     CDS             1057..1638
                     /locus_tag="MJB72_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8264324.1"
                     /translation="MSNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(2218..2895)
                     /locus_tag="MJB72_003262"
     CDS             complement(2218..2895)
                     /locus_tag="MJB72_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY8264325.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(3084..4448)
                     /locus_tag="MJB72_003263"
     CDS             complement(3084..4448)
                     /locus_tag="MJB72_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8264326.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTGLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            4576..5097
                     /locus_tag="MJB72_003264"
     CDS             4576..5097
                     /locus_tag="MJB72_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY8264327.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEKNISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1986 a          675 c          848 g         1732 t
ORIGIN      
        1 tgggtttaac ttgtggttaa tagagtaagg tgtaactaaa gataaacttc aatatgggtt
       61 taacttgtga ttaatagagt aaggtgcaac taaagataaa cttcaatatg agcttaacct
      121 atgattaata gagtaagaat gtaaatagag ataaacttca atatgggctt aacttgtggt
      181 taatagagta agagtgtaac taaagataaa cttcaatatg agcttaacct atgattaata
      241 gagtaagaat gtaaatagag ataaacttca atatgagttt aacttgtggc taatagagta
      301 agagtgtaaa tagagataaa cttcaatatg ggtttaactt gtggctaata gaataagaat
      361 gtaaatagag ataaacttca atatgagttt aacttgtggc taatagagta agagtgtaaa
      421 tagagataaa cttcaatatg ggtttaacct gtggttaata gagtagggtg taactaaaga
      481 taaacttcaa tatgagttta acttgtggct aatagaataa gattgtgaat aaagttatag
      541 ttttagatgg gtattaacct gttactaata gaatatgatt aggtgtaaaa ttatagagct
      601 gtataagaaa atattataga tatattaaat attagataat aaatatttat atagatacac
      661 aaaagaaaag gcgatatgaa gctaaactta atgacttcat atcgtttttt atttgaaact
      721 cctttgtatt tagagtgtga tttttaatat tgaggtttta tgtgattaag tcacagataa
      781 aaagtaagta tcttgatata attcttatag attaataata tagggaggtt attatgtcta
      841 ataaaaaaaa gaagtatgca aatattttag caaatgagtg cgttgcttgt ggtagctgta
      901 taaaagcatg tccaagaagt gctatctcag ttccatgtgg cattagtgca aaaattaata
      961 gagatttatg tgttggatgt gggatttgtg aaaagatatg tccagcatct gtaattgaga
     1021 ttataacaat attaaaagag gaagaaggta agtgtcatga gtaataaaaa acattggtat
     1081 gactatttat ggattttttc ttcaatatat ttgatattgg gatttgtaaa tattattttc
     1141 gcttggattg ggctaatttg tttctttgtt ccactagcta tttctattgt taagggaaat
     1201 aaagcttact gtaataagta ttgtggaaga gggcaactgt ttaatttgct tggaaataaa
     1261 ttgaagctgt caagaaatag agatattcct aaatttatta gaagtaagtg gtttagatat
     1321 gggtttttaa cattttttat ggtaatgttt gccaatatgc ttttttcaac ttatttagtg
     1381 tttgaaggaa gtagaaattt taaagaagta gtgactttgt tgtggactat aaaaatgcct
     1441 tggcaatggg cgtatcatgg aactttagtt tcaccttggg tggtacagtt tgcatttggt
     1501 ttttatagtg ttatgcttac atcaactgtt cttggtttga ttactatgat attgtttaaa
     1561 cctcgttcat ggtgtgttta ttgccctatg ggtactatga ctcagatgat atgtaaggca
     1621 aagagtaagt ctgtatagaa aggctttatg tgatatttac agttttgttc aagtagagat
     1681 gagtagtagg tttgaatata tatgacaaat atatgagtat aggggcatag atttagtttg
     1741 tgtaaggtaa actgatttat gcttcttttt tatgtcttag attattggct aagtggaaac
     1801 aatgaattta gatattaaga attattcagt tatttgaaga aaatagaata ataattaaca
     1861 ttatatgaat ggatattcat tacatataag ccagttaata gcaagattta tttaatatga
     1921 aataggtcaa gtatttaaac taaataaggt catgacaatt aatataagta tagatataaa
     1981 aagattagaa gttataactt ataaaaggta ttctatataa atagtatgtg gaaaatagtc
     2041 aaattaactt attagaaaca aaaaatatac ataaaaaaat aaatgtaaat atcaaataag
     2101 atactatata aaaatagata taagtatctt ttttattatt gaaattatga aaatatattt
     2161 gatttcacac aaaaaagagt gcctcatgaa ctaaaaagtt cattttgaga cacactttta
     2221 cagtgtcaaa gaacgaagtt tatccttatc caatactttt ataccaccac gagataaaga
     2281 cacaatacct tctctagcaa aatatttcaa cattctcgat acaacttctc tggcactacc
     2341 catatactta gcaatttgct catgagtcat atttatagtg ctactgccat ttttagctat
     2401 ctcatcaatt aaaaaagatg ccaaacgctt atcaaaactc ataaaaagaa tttgttgcat
     2461 agcccacata acatcagaaa aacgttcggt agctaattta tatgaaaata actctgcatg
     2521 aatattttct gtagataatt ttgcaaaaac agaggaacta atctgtataa tatcgcagtc
     2581 cgtttcagca tctacataga catcaaaagt tattgttttt agaatacagg aagctgataa
     2641 aatacataca tctccatcat caagtcggaa cagtgtaacc tctctacctt catctgaaag
     2701 tatgtatgta cgtactgttc cagactttat aatcaaaata ccaatacagt cactatcacc
     2761 acaatgtact ggattccctt ttttgtaatg cgaggtattt gctgaagaaa ttaataattc
     2821 tttttgtaag tcagtaagtt tatcccaaaa aggcaagcta tgtgtaagta tatcaatttc
     2881 attttgtgaa atcatacaat atctcctttc ttaaattgtt ataatcccat gatacaagtg
     2941 aatagataaa ttgacaagta gtttgttaag aggattattt agtcagatag taatagtatg
     3001 atttatgtag aaaaaatatg ataatatata ttcttgctat taatgagaaa gtaatatgtt
     3061 ttgatgatag taggtttgta taactaatgc ataccgttta tttttaaaag attaagttcc
     3121 ttatgtaacc ttatagacat aaaaccagca attatagcag ccatcaaatc agctattggt
     3181 tgtgcatata agataccatt taacccatag aatacaggaa taatcaaaat tataggaata
     3241 aaacagacac cttgtctaca catacctaaa aatccacctt cctttgcttt tcctagtgcg
     3301 aggaataatg tagaatacac tgtatagaat ccaaatagca taaatgaaaa cccatttgcc
     3361 ctaagagctt tactaccatt ttctattaat agaatgtcac cacttgtaaa ttgtgaaatt
     3421 atagattttg ggaagagtat caatataaga ccaagcacta cacaaaatat tgtagtccat
     3481 aatatggaag tttttattgc tatatgtaat ctatcgtagt tctttgaacc aaaattataa
     3541 ccagcaattg gttggaaacc ttttataaaa ccaaatacaa caagactacc taaagacgtt
     3601 attcgtgtta cagcacccat tccagctatc acagaatcac catactttgt agcctccata
     3661 ttagttaatg taatggcaag accagtcaat aattgaaata ttaaggtagg tatgccaatt
     3721 tttaggattt ctgaaaaaat ttcttttgaa aaacagcatt cgttaattct aaaactaaat
     3781 atacttttct tgcttaaaat atatcctaca taaacaagtg ttgaagcaat ctgtgagata
     3841 gctgttgcaa tagaagcacc ccttaccccc agaccaaaag tataaatgaa tatcgggtct
     3901 aaaatcatat taagcaaagc acctaataac atagcacaca tagttgtttt agcagctcct
     3961 tcacttgtga caatattagt catagtgaca ttgaatatat taaaaataga tgacacgaca
     4021 taaatgctag cataagatat tgcgtaaggc ataatacttt ttgtagctcc taacaatttt
     4081 aaaattggat tgagaaaaat cataatcaca ataataacta atgtacccac tataatacta
     4141 ctgtaaatag atgtacttac cacttttcct gctttttcat tatcaccatg ccctaaaagt
     4201 ctggcaatat atgaagcagc tccatttcca aacaataatc ctaatcccac aacaagctga
     4261 cctaatggaa atgcaacgga tatagctccc atttgacttg ttccaagtcc acccacaaag
     4321 tatgcatcaa ccaaattgta taatgcagtt atcatcatac ctatcatggt aggtaatccc
     4381 atagataaca atgcttttga gattggggca gaccctaata attcaatttt tttgttactt
     4441 tctttcatac ttaacactcc tcttctattg acattaagtc taaattataa tactataaat
     4501 tatagtaagt atggaaaata gtactataat ttatagtatt tgtcaatagt aagaaaacta
     4561 aaggagatga tttttatggc tacaatagat ttaattgtat tgggaatagt aaagcaacaa
     4621 cccaagagtg cttatgatat tcaaaaggca gtggaataca ggaatatatc acgttgggtt
     4681 aaaataagta caccatcaat ttataaaaaa gtaattcaat tagaacaaaa gggatatatt
     4741 gaaagtaaaa ctgtgaagaa tggaaaaatg ccagaaaaag cagtttattc attaactgaa
     4801 ttaggtgaga cacatttttt agatttaatg tttgatacat ctagcaaacc aataaatatt
     4861 cttttagatt ttaatgctgt tatagtaaat ttaaatagcc ttacaccaga aaaacagaaa
     4921 ttatgtctca ataatattga agagaatgtt caagagttaa agaaaactat tgagaaaaat
     4981 ataagtataa agactgaagt accagagaca gcaaaagcag tattggagca acagttggtg
     5041 ctagcacaag cattggaaac ctggataaat tctttaaaag ataatatggg aaattagaat
     5101 tagagaagct aacttaataa tcattaaaca aaaaccagca tatttaataa tgagatatta
     5161 gaaaaaataa aacaaatgat ataaactgaa aagtatattt aaaataaagc tcacttaatt
     5221 gagggagaat gttaaacttt t
//
LOCUS       DAGSHT010000224         5237 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGSHT010000224 DAGSHT010000000
VERSION     DAGSHT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.230"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..866)
                     /locus_tag="MJB72_003265"
     CDS             complement(<1..866)
                     /locus_tag="MJB72_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBY8264328.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIAKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSVELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEH"
     gene            1114..1431
                     /locus_tag="MJB72_003266"
     CDS             1114..1431
                     /locus_tag="MJB72_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8264329.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            1439..2686
                     /locus_tag="MJB72_003267"
     CDS             1439..2686
                     /locus_tag="MJB72_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY8264330.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            2715..3068
                     /locus_tag="MJB72_003268"
     CDS             2715..3068
                     /locus_tag="MJB72_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY8264331.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            3090..4535
                     /locus_tag="MJB72_003269"
     CDS             3090..4535
                     /locus_tag="MJB72_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY8264332.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVIEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDNYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            4634..>5237
                     /locus_tag="MJB72_003270"
     CDS             4634..>5237
                     /locus_tag="MJB72_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264333.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADVI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRY"
BASE COUNT         1938 a          547 c          915 g         1837 t
ORIGIN      
        1 aaatgttcat ctatataatt tggcaatgac ttagtatcca tttcatgata atttaagact
       61 gtggttttgt ttgttaactg aaatactaag tagtataatt ctacactagt taaagttata
      121 ttgtattcag actctaaaaa atttgatata tctcttgaaa tgcttatatc attattatta
      181 tctatgtagt ttactacttc gacagattct atgtagttat tcttttttat tctgtctata
      241 gttatcatta aatgaagaag tatgttattt atagcatact catttataaa caaattgtat
      301 ttttttaata tatatattat attttctttc agtttggtca aattatactc ttgatatatt
      361 ttttgtacat caaataagct taaaaaatca cttcccgttt ctttaaatat tatagagctc
      421 attaatttgc gctttgcaga ctcatttcca attagtttca ataaaaattt cgattgcttt
      481 atctttaaat tataacttcc tataaattta ttagctttgg ctatatctcc ttcaatagta
      541 gctggactaa caaacaattc ttcacttaaa tcaaatatat taattccatt actattaact
      601 ataagttctt ttaaaattaa atttaacctg ttatcaggtg atatattttc tgaactatca
      661 accttggttt gacaattatt attgagattt actttatatc cttttttaga ggataatata
      721 aattcaccat tggaattaat ttcgtttaca tattttctaa ttgaacgcgt tgagacatta
      781 agataattag ccaatgtaga tgaatctatc caatcatgtt gtgtttttat gaaatttaaa
      841 aatagttctt ttttagatgt tttcattaga ttccctctca tagttttaaa ataaaatctg
      901 tatgatatga tatttatata attgaatgta cgtattataa tgtacattat actatatatt
      961 tatgtgtctg gttaagagta aatgatttcc tattattagg aaatatattt atgtgaaagg
     1021 aaattattta ggaagtacaa tataaataca atcaaatcaa ataaaaattc attaagaaat
     1081 aaattaatgg ataaaaatac tggaggtatt gttatgaaga atataaaagt gttgttggca
     1141 tgtggctcag gagcaagtac aggttttatg gcaactagta tgagaaagag tgcaaagaaa
     1201 aaaggcatag aagcagatat aaaagctaga agtgagtcag agatagataa atatttacca
     1261 gaaatagatt gtttattatt aggaccacat ttagattata tgaaagatga aatacaaaaa
     1321 aaagctgatg aatataatgt tccagtagca tgtatatcta aaacagcata tggaagatta
     1381 gatggtgtaa aggctttaga agaagcatta gaattaatat taaacaaata aaatatctat
     1441 ggggagattg tttatgaata aatttataga tttgttaagt gaaaagttta ctcctgttgt
     1501 aaataagata actaagaata tatgggtaca atcagttcaa tcaactataa tgaaagtttt
     1561 gcctatggta tttgtaggtt ctttagtaac tatagtttct gtcttgaaga attacataag
     1621 tttcttacct gatttaagtc caataaatca atatacattt ggacttttag gactatttat
     1681 agcattttta ttacctatgg aaataatgaa gaataagaaa tttgaaagta tgagtgtagt
     1741 agctggtctt gcaggtgctg gattaatgtt aatgatgatt cgtccagaga ttacgaatga
     1801 aggagctata tttaacttca atagatttgg aggagaagga atgcttgtag cactagttgc
     1861 aggattattc tccggattaa taatgtcttt atttgggtct ttctcatttt ttggtgaaga
     1921 ttcagcactt cctgattttg taagtaagtg gtttgatagt atgttaccta tagcagtaat
     1981 aatgtttata ggttggggac taatttatgg acttaacttt gatatgtttg aaataatagt
     2041 taggatattt aaaccagtat cagatatagc acaaacattg cctggatttg tactattaaa
     2101 cttcttagta gtatttttat attctttagg tataagtgga tgggtattat ccccaataac
     2161 ttatgcaatt cagcttagtg caattgcagc taatgcagca gctgtagcat caggagggct
     2221 tgcaactaat atatatactt atgaagttgt tcaaacaggt tggataactt taggtgggtg
     2281 gggagcaaca ttaccactag tgctaatgat gtgtttatct agagctggaa gaataaaagc
     2341 aatcggaaga gcctgtatcg ttccttctat aatgaacata aatgaaccag taatgtttgg
     2401 agctccagta gcatggaacc catttttaat gttacctatg tggataggaa cagtagtaat
     2461 atcaataact acttacttat caatgagtat gggatttgtg acaattccga gtaaggtgtt
     2521 taatttatgg tatgttcctt ttggtatatc tacttttatg gtaaatcaag atgttagagg
     2581 gcttattgtt ttagcaataa atatagtatt gctattcatg atatggtatc cattttttaa
     2641 agcttatgaa aaagaagaac taaaattaga gagtcaagaa gcttaaattt agatgttgaa
     2701 aggaaattat aaatatgaca actgaagaac atgtaataga tgaagaatta gtagaagtag
     2761 caatgcaaat aatacttcgt gcaggtgagg ctagaacaga aattaaacat gctcttaatg
     2821 atttagaaag gtttgattat aaaaatgctg atttaaaact agctaaagca aaagagttta
     2881 tgacagaagc acatagagct caaactaaca ttatacaagg ggaagctagt ggagaaaaaa
     2941 gagcacattc acttttattt gcacatgctc aagatactct tatgacaata ttcagcgagt
     3001 taaatataac tatgtcttta gtaggaatag taaagtctat agaatataag gtaaataata
     3061 gtaaatgatt taaataaaca aaataaggag tgattaaatt gagtaagttt cctaaagatt
     3121 ttttgtgggg tggagctgta gcagctaatc aattagaagg tgcttggaat gaaggtggca
     3181 aagggtggtg cttagctgat gtacatatgt atgataaaga tagagatata tctaaacatt
     3241 acgagtctga tatgactgta gataaaataa agtttgctat gaaagataat aatgggtatt
     3301 atcctaagcg taatggaata gacttttatc atacatacaa agaagattta aagcttttga
     3361 aagaactagg gttaaagtgt tttagatttt caataaattg ggctagaata tttccaaatg
     3421 gagatgattt agagcctaat gaagagggat taaagtttta tgataatctt ataaatgaag
     3481 taatagataa tggtatggaa cctattgtaa cactactcca ttatgagact cctttgaatt
     3541 tagtattaaa atataaagga tggtctaata gaaaagtagt ggatttcttt gttagatatg
     3601 cagaattaat attaaataga tacaaaaata aagttaaata ttggatagtt ataaatcaaa
     3661 taaatttaat gtatcatgaa tcatttaatt cagttgcatt ttgtgtagac caggtagatg
     3721 atgtagaaga ggccaaatat caagctattc atcatcaatt catggctaca gctttaacta
     3781 agaagcttgg aaaagaaata aattcagata tgatgatagg gatgatgatg gcagattgta
     3841 catcttatcc agaagactgc gaccctgaaa atgtggtgtt tgctttaaaa agaaatagaa
     3901 tacaatattt tttcactgat gtaagtttta ggggagagta tccagtctat attaagagat
     3961 ttttcaaaga aaataacata aatatagtta tagaggaagg agatgataaa atcttaaaag
     4021 aaaatactat ggattttcta gctttatcat actattattc tacaacagta tcagcaagta
     4081 aaaacaccat gaatccaact gataatataa agaatccata tttaaaagca aatccttggg
     4141 ggtgggcaat tgatcctaaa ggattataca atactttatc tcaatattat gatagatacc
     4201 aaaagccaat aatgatagct gaaaatggat ttggaatgca tgataaattt gaagatggaa
     4261 aagttcatga taattataga atagattatt taggaaaaca tatagaacaa ataggtgaag
     4321 ctatagctga tggaaccgaa atattagctt attgtatgtg gtcgccaata gatatagtaa
     4381 gttgttcatc tcaagaaatg gaaaaaagat acggatttat atatgtagat atagataaca
     4441 atggaaatgg aagtaaaaaa cgttataaaa aagatagttt ttattggtat caaaatgtaa
     4501 taagaactaa tggagaagaa ttaaaagaaa agtaaatagt ttttaaatat aaataatccc
     4561 tcttataaat actatgttat taatacagtt ttataagagg gattataatt ataaataagg
     4621 tggtttatta attatgaatg tcataaaaaa atttatattt gtaattttaa ttataaatat
     4681 actgggatgt ggtatatctc ttatacttca gtcaagcctt ggtagtgatg caattacact
     4741 tttaaatgag ggtatacata taaagttagg tataagttat acaatagcag ggctaatata
     4801 taatggaact ctattactta tagcattgct attgaataaa aaaagtttgg gattaggaag
     4861 ctgtgtttat gtactagtag caggaatatt tatagatttg tatatatact tattattaga
     4921 ttttaatata agtagtttag ggattatcat tagaataatg agctttataa ttggacatat
     4981 acttatgtgt agtggatttg caatgcttat aaaatttgac attggaatga gccctttaga
     5041 tgctatatta atctatattg agggcaaatt taagtatcca tataaagttt taaaaactat
     5101 agcagatgta atatttttaa ttttaggtgt atatttaggc ggaaatttag ggtttggaac
     5161 tatattttca atattatgta ctggaactac aatttcaatt gttagtaaga tgatttctgt
     5221 taataaaaaa aggtatt
//
LOCUS       DAGSHT010000225         5205 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.231, whole genome shotgun sequence.
ACCESSION   DAGSHT010000225 DAGSHT010000000
VERSION     DAGSHT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.231"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(20..583)
                     /locus_tag="MJB72_003271"
     CDS             complement(20..583)
                     /locus_tag="MJB72_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264334.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(742..1455)
                     /locus_tag="MJB72_003272"
     CDS             complement(742..1455)
                     /locus_tag="MJB72_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBY8264335.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1747..3411
                     /locus_tag="MJB72_003273"
     CDS             1747..3411
                     /locus_tag="MJB72_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBY8264336.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3518..4417)
                     /locus_tag="MJB72_003274"
     CDS             complement(3518..4417)
                     /locus_tag="MJB72_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBY8264337.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4444..5082)
                     /locus_tag="MJB72_003275"
     CDS             complement(4444..5082)
                     /locus_tag="MJB72_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBY8264338.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         1792 a          793 c          615 g         2005 t
ORIGIN      
        1 aaaactctaa atcaagtttt taaatgtttg cgatttttga tacataagaa tgttgtctag
       61 aattatattc tgataataaa ccttcatact ctaaggttaa gccagataaa ttataaaaat
      121 catttggcgt tacttcaaat gcttgtttat ttaaaatgtc tatctcactt ttttctaaca
      181 tgtcagcaac aaaatttgaa catacatatt tatattcaga ttgtctctga atattcatta
      241 gtaaatatat taatgccatt gtatcgtaat agtaatcttc tctataatca ctaattaatt
      301 ttatattttt atatagattt tcatattgta aattatctac ttctaacgaa tataccctac
      361 acacagtgtt ttttcttata gcatataatc cttgatttat attttcttca acaaatcctc
      421 caataaatgg attccttgga tttagccttc caaaagaata cattggtttt agattttcat
      481 ttaatgatat tgacacatgt gtatacagct ttttacttat attccttatt aagtaagata
      541 atatagttcc tgtatatgtt gttacaatat agactttatt catcatttcc ccccaatcta
      601 gtaactaaat aatattatac acttacaatt taatatcata agatattcta acataataaa
      661 tgtggtttta caaattttga ttttaaggta ctttaatatt atataatata ataaaaatat
      721 aaatataagt tttctatatt tttaaccaac agatgttttc ttcaaattaa tcagatgatt
      781 tagtatatct tgaacctttc cagagtcctt taaatatcct aatgccccat ttatttgtga
      841 ttttaaatct atttctactt ccatatccaa atcaacattt ccttttttta tagatatatt
      901 tttaagtgtt attgggtaaa gataactttt ttccaaagta atcttatttc cattaacctc
      961 aaatggaact tttttattgt agttacttaa tattttttct agcattttat cacttagttt
     1021 aaattttcca attttcacat tctcaagact tacaactaag tttccatcct ttaaagtagg
     1081 tctcaattca agttcatatt gactatttac caaaccaaat accttatatg gtcctgctac
     1141 ctttatttta ccatttttca tttcaacaaa attattttca agttcattaa ttccatgctt
     1201 ctgcatcaat gtatatatta aatttttaaa ctcttcctct gaaacagata tttttccaac
     1261 cattctaagt ggatttttta ctacctccat agaatttata tatgagcctt ttgataaata
     1321 ttcttcatct atgattttat tattttgtga actaacatca tatctttctt ttggtgtcaa
     1381 gatatataca acaattcctg caagtactaa aacaataaca attaaactta acaaaatctt
     1441 tgcaaccttt cccataatcg tctcctttac aaatctgtca tcagatttaa aataaattta
     1501 gataactaac ttcacataat attcttaaca atatacatac tataatacag tcttttaaaa
     1561 attaattcaa ccaatctaat gttttgtaaa taaactgtaa aaacatgttt ttagtataaa
     1621 agttaatatt ttgcataata ctcttaatta cacctttatt tttttctaat cttggattaa
     1681 aattaatatt atgcaataca gtgtagtgtt ctgtattata atcaaattaa ataggaggta
     1741 tagaatatgt caaatgaaac gaactcatct aactttataa agaatattat tataaacgac
     1801 ttagaaacag gaaaacatga cagtatcata actcgttttc cacctgagcc aaacggatat
     1861 ttacatattg gtcatgcaaa aagtatatgt cttaattttg gattagctaa ggagtttaat
     1921 ggaaaagcta atttaagatt tgatgataca aatccattaa aagaggatgt tgaatatgta
     1981 gagtccataa aagaagatgt aaagtggtta ggatttgatt ggaacgagtt aaattttgca
     2041 tctaactact ttgatgaaat gtataagaga gctttaatac taataaaaaa gggtaaagct
     2101 tatgtatgtg atttgacaca agaggaaatg agagaatatc gtggtactct aactgaacca
     2161 ggaaaagaaa gtcctcatag aaacagaact attgaagaaa accttgattt atttgaaaga
     2221 atgaaaaatg gtgaatttaa agatggtgaa aaaacattaa gagccaaaat agatatgtct
     2281 tctccaaata taaaccttag agacccaatt atatatagaa tatctcattc aacacaccat
     2341 aatacaggag ataaatggtg catatatcct atgtatgctt ttgctcatcc aattgaagat
     2401 gcaattgaag gaataactca ctctatatgt actttagagt ttgaagacca aagacctctt
     2461 tatgattggt ttgttaaaga atgtgagatg gaaaatatac ctagacaaat agaatttgct
     2521 agacttaata taaataatac tgtcatgagt aaaaggaaat taaaacaact tgtagatgag
     2581 ggtattgttg atggttggga tgaccctcgt gttcctacaa tatccggaat cagacgtaag
     2641 ggatatactg cagaagcatt gcgcaatttc tgtagtgaaa taggggtatc aaaagttaac
     2701 tctacggtag atagtcaaat gcttgattat ttcctaagag aaaacttaca accaaaggct
     2761 cctcttacta tgggagtttt aagaccttta aaacttatta taactaatta cccagaagat
     2821 aagattgaga tgcttgaaat agagaataat gctaaagatg aatctcaagg aaagagatta
     2881 gttcctttct ctagagaatt gtatatagaa caagatgatt ttatggaaga acctgttaag
     2941 aaatatttta gatttttccc aggaaatgaa gtccgtttaa aaggtgctta ttttgtaaaa
     3001 tgtactgatg taattaagga tgaaaatgga aatgtagttg aaatacatgg tacttatgac
     3061 cctgaaacta agagtggctc tggctttaca ggacgtaagg taaaatctac aatacattgg
     3121 gttgatgcta aatcagctat accatgtgaa tttagattat ttgaaccatt aattcttgat
     3181 gatatacctg aaaatgaagg taaacatttc ttagaacaaa taaatcctaa ttcattggaa
     3241 atattacaag gttttgttga acctacacag ataaaagatg ctaaaccttt tgataaattc
     3301 cagtttgtta gaaatggatt ctttagtata gataataaat atacaactga tgaaaaattt
     3361 gtatttaata gaatagttcc tctaaaaagt tcttttaaac ccggtaaata atcttacaat
     3421 caaaaaatga agacttaaaa tataaattac ttctactata aataaactta gttagaagta
     3481 attttttatt tgagtcttca tcatttactt taaatgatta acttttttca ctacagattc
     3541 ccatgacttc tattccatct tttatgcatt ctatgttaag aggaaaatct ggtccttttt
     3601 ctccgtcaat atctgttata agtgaatctt cacattctat atgcaccttt ttagttttaa
     3661 agtatagtat ttcatttcca ttatattgat ctaaataatc acctctaagc acacttatca
     3721 aaactggtat tgattttggt attggcattc ctttaaaaat aattacatcc aaatatccat
     3781 catctatttc agccttatat gctagattta tatttcctgc tgttttgcca ttaaaaatca
     3841 acataagata catatctcca tcataataca tttcctcaga agatactttt atcttaaatt
     3901 tcctcatata caatgcttct tctattccct ttatataata agaaattctt ccaaatgaat
     3961 tttttaagtc tgtgtttatc ctttgtgata catccgtaaa cataccagca cttgcaacat
     4021 ttataaagta cttattattg actttcccta tgtctatttt ctttggtttt gaatttatga
     4081 tttcatttat agcttctttt atatcaaatg aaatttgaag agcatttgaa aaatcatttg
     4141 cagttcctgt aggcaaaatt ccaattggaa tttttatgtc taattctttc attgcattta
     4201 ctactaaatc tacagtacca tcaccaccag ctatcaatat gtgttcataa gaactatcaa
     4261 cttccttaaa tgcattcttt acaggttctt taatagttaa tctatagggt atcaataaat
     4321 aatttttttc ttgatataat tctatgatag tgtctaaatt tgataatatc tttttttcac
     4381 ctgaattagg attgtatata agttttactt ttttcattat ttctccttac tcattttatt
     4441 cttttagtat ccaaaatctt catttaaaaa aatcacatgt attctttctg gtgttaattg
     4501 tctttttata agtgtataac tacagcatat tcttcctgga cttgaacagt ctatacatat
     4561 accagtagtc ttacatccag tatttgtact taatctttta caattggctg gagcagctat
     4621 tgtttttgtt ctttttatag cttcatctac atctttaact attttattta ctcctgctat
     4681 aactattact ttatcaggtc catatatcat agcagcaacc ctattcccat tcccatctaa
     4741 attgtaaagt tctccatcct ctgttatcgc attagtactt gtaaaataag catctgcaga
     4801 aaatgacttt ctatatattt ctttcatttc ttctggtttg atatcttttg catatctatc
     4861 aagaaaatta tatttttctc ttataaaatc aatcactcct gtttcaaata aggtcataga
     4921 acctccacat cctactagag aacctgcttt aactatttct tcaatttttt taagaagctc
     4981 ttctttgtta cttactaaat accctttcat attattgttt tccaaagatt ttattgtttt
     5041 ttctattctt ttttcattaa gccaatttaa attttgatcc attttaactt cccccttatt
     5101 tttaatatgt acttaattat ttataaactt tattttcttt ataagtttat tttagtacat
     5161 ctaaaaaaaa atacaatata aaacttaaat agttttacat aattt
//
LOCUS       DAGSHT010000226         5163 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.232, whole genome shotgun sequence.
ACCESSION   DAGSHT010000226 DAGSHT010000000
VERSION     DAGSHT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.232"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            122..622
                     /locus_tag="MJB72_003276"
     CDS             122..622
                     /locus_tag="MJB72_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBY8264339.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            679..1665
                     /locus_tag="MJB72_003277"
     CDS             679..1665
                     /locus_tag="MJB72_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY8264340.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1715..2437
                     /locus_tag="MJB72_003278"
     CDS             1715..2437
                     /locus_tag="MJB72_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBY8264341.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2466..4400
                     /locus_tag="MJB72_003279"
     CDS             2466..4400
                     /locus_tag="MJB72_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8264342.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4393..>5163
                     /locus_tag="MJB72_003280"
     CDS             4393..>5163
                     /locus_tag="MJB72_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264343.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDN"
BASE COUNT         2149 a          391 c          896 g         1727 t
ORIGIN      
        1 gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt tatagatttt
       61 gtataaaata aagttatgtt attaattttt ttaaaaataa aacttttact aggagacaga
      121 tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa tctatgcagt
      181 tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg tagcagagat
      241 tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat taggttgttt
      301 tttagcaaat gtaataggac catatggtgt acctgatata atttttggaa cattagccac
      361 atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaga ataaaagtac
      421 tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg gtctaatgtt
      481 aaatgtattc tttgggcttc cattaattct atctatgata caggtaggtt ttggagaatt
      541 tgtagtagta acaattgtag gagtaccatt tttctgtttt ttaatgaaaa aatataaaaa
      601 tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag agtattatta
      661 gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg gatatatatt
      721 aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct ttcagccaaa
      781 acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca agcagaacaa
      841 ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt tatatttaca
      901 gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta taattataag
      961 ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg taaaagcagt
     1021 ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa tgatactaat
     1081 gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc agaatctcta
     1141 attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt atgccctata
     1201 ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa aagtgataaa
     1261 ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga aagtaaaaaa
     1321 atacatataa acttggaaga atggttacat gaagtatata acaatttata tggaaatagt
     1381 tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac taaagaaggt
     1441 gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga agatggtagt
     1501 ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc gattttattt
     1561 tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct actttatgat
     1621 aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt agaaatgttg
     1681 aaattaaata attattaagg agatatacgt tataatggaa aattttaata agaatgctaa
     1741 aataattggt attggagctg aaggtattaa tataataaat gaagtagaag aaaaaataaa
     1801 agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag aatatgttag
     1861 aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag ataaacatat
     1921 tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc tatctatagg
     1981 aatggattgt tcagagaaag aaaataaaga agatttagag cttgggagag agtttaaaat
     2041 caataatgat agtatattta aatttgttga tttaatgaat atcatggtag agtctatttc
     2101 tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg taggagataa
     2161 aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg agatagctga
     2221 cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga aaggaattgt
     2281 atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa taagtaatat
     2341 tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa aggaaaattt
     2401 aaatggaaat attagagtag gcttattgta taattagtat attttagata ttaaggagag
     2461 tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa tttttttatc
     2521 aggaaacaga cacaccacag atttttagag aagaaagcat aattgcactt ggatttgata
     2581 taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag tatattaatg
     2641 agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa ggagattatt
     2701 ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg tctattggca
     2761 tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg catacactac
     2821 cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat acaagtgaat
     2881 tttataaatt aaataataat gatattagct catatttaga aattattaag tcacaagata
     2941 agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat ttaaaaaact
     3001 attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt cagcccatta
     3061 atctagaaca attagaaagt ctaaaaaata taaagacaga tgattatatt ttattgtata
     3121 atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt ggcgtatgtc
     3181 aagttatgga agggttcgat aaagagaaag taagtgcttt ggagggtgga tatttttttc
     3241 ctgttagatg gatagagtat gaaaaggaga atatagacgg aaatgcatgg gttatatttg
     3301 aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg ccattaaata
     3361 ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag atggtttaca
     3421 atctagtaag tgaaaccagt ctaagaaaca gatactatac aggttactta gattataaaa
     3481 acaatgtaga attttgtaca tttcatcaat catatggata tgaggaattt atagagggtc
     3541 ttaagtctga tggtgaaggc aattttaagc cagaagatgg gatattaaag gatatatcta
     3601 tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag ttggaaaaag
     3661 atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta gaaaatatag
     3721 aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata atagatgaaa
     3781 ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg gaagatgata
     3841 aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag gaaaaatttt
     3901 ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa tcaatagcac
     3961 ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca gattatgatt
     4021 tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc aataaaagga
     4081 tagagttttt atatgataga gaccatgtta ttggacaagc ttatctactt gctgtagaaa
     4141 atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt caagagtatt
     4201 tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga aatgaaaaag
     4261 ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa aataataatg
     4321 cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct agtgaggagc
     4381 taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg gataaggata
     4441 ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa gtatctagag
     4501 aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa acttagattt
     4561 ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact tcctaaattg
     4621 tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct ttctatatgt
     4681 aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa ttataatctc
     4741 ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa aaaaggtatt
     4801 tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa aatattatta
     4861 agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga atatgatgag
     4921 tatagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc tattttatgt
     4981 agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta ttttcaaaat
     5041 gtagatttaa tttatataga tagaaaaaaa ctattggatt ataaatttta taaaaacaat
     5101 gatagattta aagattgtta tttactagct agacttctac ttttaaattt gtctatggat
     5161 aat
//
LOCUS       DAGSHT010000227         5134 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.233, whole genome shotgun sequence.
ACCESSION   DAGSHT010000227 DAGSHT010000000
VERSION     DAGSHT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.233"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            287..556
                     /locus_tag="MJB72_003281"
     CDS             287..556
                     /locus_tag="MJB72_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY8264344.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(774..1019)
                     /locus_tag="MJB72_003282"
     CDS             complement(774..1019)
                     /locus_tag="MJB72_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBY8264345.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1445..2143
                     /gene="tcdC"
                     /locus_tag="MJB72_003283"
     CDS             1445..2143
                     /gene="tcdC"
                     /locus_tag="MJB72_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBY8264346.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            complement(2478..>5134)
                     /gene="tcdA"
                     /locus_tag="MJB72_003284"
                     /gene_synonym="toxA"
     CDS             complement(2478..>5134)
                     /gene="tcdA"
                     /locus_tag="MJB72_003284"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBY8264347.1"
                     /translation="SKLVKGLININNSLFYFDPIEFNLVTGWQTINGKKYYFDINTGA
                     ALTSYKIINGKHFYFNNDGVMQLGVFKGPDGFEYFAPANTQNNNIEGQAIVYQSKFLT
                     LNGKKYYFDNNSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNPDTAIISK
                     GWQTVNGSRYYFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSTSNGFEYFAPANT
                     YNNNIEGQAIVYQSKFLTLNGKKYYFDNNSKAVTGWQTIDSKKYYFNTNTAEAATGWQ
                     TIDGKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQ
                     IGVFKGPNGFEYFAPANTDANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIIN
                     NKKYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMVTG
                     VFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDGK
                     KYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNTNTFIASTGYT
                     SINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTHNNNIEGQAILYQNKFLTLNGKKY
                     YFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTSIASTGYTIIS
                     GKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFG
                     NNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGSNGFEY
                     FAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYFMPDTAM
                     AAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         1803 a          721 c          711 g         1899 t
ORIGIN      
        1 tgttgtaaaa tatattcaaa gggatttaaa ctaatctata gtcttgtaga atattcaaag
       61 agatttaaag caatctataa tgctgtaaaa tatattcaag gggatttgaa gtaatttatt
      121 gtgttttgaa atattcaaaa agatttttgg aatagttttt ataaatttac cagtactaat
      181 aactttatgt ttaacattta gatgacctaa aatcaactat ttgcactttt gtattcatct
      241 aaaatcattt tataatattt ttaaataatg gaaaggatgt ttgaacatgt ttgaatcaag
      301 atgtggtgtc tgttgtgaca gttgtacaaa aaaagaacaa gtaaattgca ctggttgccc
      361 aactatggaa aaaccatttt ggggtggaga atgtaaggtt aaaacttgct gtgaaagtaa
      421 ggaacttaat cattgtggtg aatgtgatac tttcccttgt gatatgcttt taaataaggg
      481 aaaagaccaa ggttttgacc ctatggtaaa tattgagcaa tgtagaaaat ggcttgaaga
      541 aacagtttct aattaattgt tttagaatgc tttgttaagt aatttattta atcctttgtt
      601 aggtagtatg tttagtcatt tgttgattgg tatatttata catttattga tgggtatatt
      661 tagccataca aatagattta cataaaattt acgactaaaa aagagttgag tattagtttt
      721 tattatttaa aacggatatt caactctttt ttatgtctaa cttttttgat tttttaatct
      781 ttccaatacc accactttaa tttttctggt tctggatttt cagttgatgc aaaatatact
      841 gcacatctgt atacatataa ctgacatctg tctatgtgct gtccacgcat taaacattcc
      901 ttttcataca tttgtgctgg gtttgcatct ttaagtgatt taatagttgt atatcctaac
      961 atgattaaag atttttctgt tgctttccct acccctggaa ttttttgtag ttctcccata
     1021 cttcaccttc tttctggtat attcttttta tattatacct attaccagat atttttttat
     1081 tatagttaat atttaatatt taatttttta ttatatcaat ttatttatgc tctttcatat
     1141 atatatattt taccacctct aaatcactga gtcacttaat tacatcataa tatagtttta
     1201 tacaaataaa atactttatg tttaatttaa tatataaaat tcaccttcaa gaaaattata
     1261 ttataatctg acatttttat cttatttttt caaaatatat tgaatcttct tactttattt
     1321 tgtaaaatta tgcttagggg aaatatattt taggaaaata tgaatatata atttttagtc
     1381 aactagttat tttaagtttt taacttttaa aataaaatat atctaataaa agggagattg
     1441 tattatgttt tctaaaaaaa atgatggtaa cgaatttagt aatgaaggaa aaggaagctc
     1501 taagaaaata attaaattct ttaagagcac aaagggtatt gctctactgg catttatttt
     1561 aggcgtgttt tttggcaata tatcctcacc agcttgttct gaagaccatg aggaggtcat
     1621 ttctaaccaa acatcagtta tagattctca aaaaacagaa atagaaactt taaatagcaa
     1681 attgtctgat gctgaaccat ggttcaaaat gaaagacgac gaaaagaaag ctattgaagc
     1741 tgaaaatcaa cgtaaagctg aagaagctaa aaaagctgaa gaagctaaaa aggctgaaga
     1801 acaacgcaaa aaagaagaag aggagaagaa aggatatgat actggtatta cttatgacca
     1861 attagctaga acacctgatg attataagta caaaaaggta aaatttgaag gtaaggttat
     1921 tcaagttatt gaagatggtg atgaggtgca aataagatta gctgtgtctg gaaattatga
     1981 taaggttgta ctatgtagtt ataaaaaatc aataactcct tcaagagtat tagaggatga
     2041 ttacataact ataagaggta taagtgctgg aactataact tatgaatcaa ctatgggtgg
     2101 aaatataact ataccaggga tagctgtaga gaaaattaat taatattgtt ttgtattata
     2161 gttaatattt tatattatag tcaatatgtt taaagatgtt tttataattg caaataaaca
     2221 gttacaaggc tctaaattag tttttgcttt tagcatatta tctatttttt atcaactatt
     2281 aattatttag tatcaatatt tccatatatg aattttatta taaaatagtc aagaataata
     2341 gattattaaa tgatagaaaa attttaacta aaagtcatgt attacaataa cacatgactt
     2401 ttaattaaat ctcaatattt attatataaa aataatttct tagtagcaca ggaataattt
     2461 tttatcaaac atatatttta gccatatatc ccaggggctt ttactccatc aacaccaaag
     2521 aaatatataa caccatcaat ctcgaaaagt ccaccagctg cagccatagc agtatcaggc
     2581 ataaagtaat atactttacc attaatagtt tgccatccag taactgcttt tgaattatta
     2641 ccaaagtaat atatttttcc aagtaaatgt aggaatctat tttgataacg tatagcttga
     2701 ccttctatat tgttagcatc cgtattagca ggtgcaaagt attcaaatcc attagaccct
     2761 ttaaacactc ctatctgagg taaaccattt ctaaagtaaa agtttttatt atcaatagtt
     2821 ttataaccat tcgcacccat agctgtatta ggctcgaagt aatatctatt accatcaata
     2881 gttacccaac cagtagccgc ttttgaatta ttaccaaaat aatatatatt gtcatgtaaa
     2941 tataggaatc tattttgata acgtatagct tgaccttcta tattgttagc atctgtatta
     3001 gcaggtgcaa agtattcaaa tccatcaggt cctttaaaca ctcctatctg cataatacca
     3061 tcagtattaa aataaaaatg tttaccacta ataattgtat aaccagttga agctatagaa
     3121 gtgttagtat taaagtagta ttttttacca ttaatagttt gccatccagt aactgcaaca
     3181 gcagtgttag tattaaagta atatttttta ccatcaatag ttcgcaatcc ggtaactgct
     3241 tttgagtcac taccaaagta atatttttta ccattcaaag ttaagaattt attttggtaa
     3301 agtatagctt gaccttctat gttattatta tgagtattag caggtgcaaa gtattcaaat
     3361 ccattaggtc ctttaaacac tcctatctgc ataataccat cagtattaaa ataaaaatgt
     3421 ttaccattaa tacttgtata accagttgag gctatgaaag tgttagtatt aaagtaatat
     3481 tttttaccat caatagtttg ccatccagta gctgcttcag cagtgttaag attaaagtaa
     3541 tattttttac catcaatagt ttgccatcca gtagctgctt cagcagtgtt aagattaaag
     3601 taatattttt taccatcaat ggtttgccat ccagtaactg cttttgagtc attatcaaaa
     3661 taatattttt tgccattcaa agttaagaat ttgttctggt aaactatagc ctgaccttct
     3721 atgttattat tgtgagtatt agcaggtgca aaatactcaa atccattagg tcctttaaat
     3781 actcctgtta ccattttaga ttcattatta gcatcaaaat agaaattatt tctttcaata
     3841 gtaatatatc cattttgaag aattccatca taactaaagt aatacttgtc attatttata
     3901 gtgcatagat gaattgcagc aatagcatta ttaggattaa agtaatattt cttattgtta
     3961 ataattctcc atccagtaac tgcttttgag tcactaccaa agtaatattt tttaccattc
     4021 aaagttaaga attcattttg gtaaagtata gcttgacctt ctatgttgtt agcatccgta
     4081 ttagcaggtg caaaatattc aaatccatta ggtcctttaa acactcctat ctgcataata
     4141 ccatcagtat taaaataaaa atgtttacca ttaataattg tataaccagt tgaagctata
     4201 gcagtgttag tattaaagta atatttttta ccatcaatag tttgccatcc agtagctgct
     4261 tcagcagtgt tagtattaaa gtaatatttt ttaccatcaa tagtttgcca tccagtagct
     4321 gcttcagcag tgttagtatt aaagtaatat tttttactat caatagtttg ccatccggta
     4381 actgcttttg agttattatc aaagtaatat tttttaccat tcaaagttaa gaatttactt
     4441 tgataaacta tagcctgacc ttctatgtta ttattataag tattagcagg tgcaaaatat
     4501 tcaaatccat tagaggtact aaacacacct attttcacta cacaatcact atcaaaataa
     4561 aagtgtttac catcaatagt tttataacca ttaaaggcaa tagcggtatc agtatcaaag
     4621 tagtatctac taccattaac agtctgccaa ccttttgaga tgatagcagt gtcaggattg
     4681 aaataatact tattattgtc aattacttgc aatccgactg cagcaatagc attattagga
     4741 ttaaagtaat atttctcatt gttaataatt ctccatccag tgactgcttt tgagttatta
     4801 tcaaaataat attttttgcc attcaaagtt aagaatttac tttgataaac tatagcctga
     4861 ccttctatgt tattattttg agtattggca ggtgcaaaat attcaaatcc atcaggtcct
     4921 ttaaatactc ccaactgcat cacaccatca ttattaaaat aaaagtgttt accattaata
     4981 attttataac tagttaaagc tgctccagta tttatatcaa aataatattt tttaccattg
     5041 atagtttgcc atccagttac taagttaaat tctataggat caaaatagaa taatgaatta
     5101 tttatattga ttaatccttt aactaattta ctat
//
LOCUS       DAGSHT010000228         5124 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.234, whole genome shotgun sequence.
ACCESSION   DAGSHT010000228 DAGSHT010000000
VERSION     DAGSHT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.234"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            138..1601
                     /locus_tag="MJB72_003285"
     CDS             138..1601
                     /locus_tag="MJB72_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBY8264348.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1657..2793
                     /locus_tag="MJB72_003286"
     CDS             1657..2793
                     /locus_tag="MJB72_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY8264349.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3029..4144
                     /locus_tag="MJB72_003287"
     CDS             3029..4144
                     /locus_tag="MJB72_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBY8264350.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4296..>5124
                     /locus_tag="MJB72_003288"
     CDS             4296..>5124
                     /locus_tag="MJB72_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8264351.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     "
BASE COUNT         1965 a          529 c          978 g         1652 t
ORIGIN      
        1 agattgtaga ttaaagataa tataaataaa tagaaggtag attttttaga tatattaata
       61 taaagattat ataaaatatt gaaatatagc tgtttaaatt attgtctata aatataaaat
      121 gaatggtggt gagttaagtg accgatttag ttaacaccat taatggaatt gtttggtcac
      181 ccgtattagt tgcaatgtgt ttgtgtatag gattatattt ttcattgaaa ttaaaatttt
      241 ttcaaataag agatgtaaaa gaaatgttta ctctcttgct tgagggagat agttctcatg
      301 ttggtatatc atcatttcaa ggttttgcta catctatggc tggaagaata gggactggaa
      361 atatagctgg tgtagctgta gcaatagcaa tgggaggtcc aggagcatta gtatggatgt
      421 gtataatggc tacattaggt tcagcaactg catttgtaga atctacttta gctcaaatat
      481 ataaagatga gcatgatggg cagtatagag gaggacctcc atattatttt gaaaaaggtc
      541 ttgggtggaa aaagtgttca gttttatttt caatagttgc aataataagt tatacatttt
      601 ttatgcctgg tacacaatca aacactttat atctagcaat gaatcaagca tttggatttt
      661 caaaaatgac aatagcagta gcatcttcta ttgcattagc gataataatt tttggaggaa
      721 ttaaaaggat tggtttattt gctgaaaaag tagtacctat aatgggtgga gcatacctta
      781 ttatgacatt agtaataatt ataacaaata ttagtttagt tccagaggct attgtaacaa
      841 tgtttaaatc tgcatttgga tttggttcag tttttggagg aatgctaggt tcagcaatat
      901 cttggggagt aaaaagaggt atatactcaa atgaagcagg agaaggttct gggacttatg
      961 gtgcggcagc agcagaagta agtcatccag ccaaacaagg attagttcaa gcattttctg
     1021 tttatgtaga cacattatta atatgtatgg ctacaggtat tatgattgta ataacaggaa
     1081 tgtataatgt tacaggtgtt tgcacagcac taccaggtgt agaaccggga ccacaatatg
     1141 tacagagtgc aataaataca ttacatccac aattaggtga tatttttata gcaatagttt
     1201 tattattatt tacatttaca actttattag ccttctcata tatgatggag actaatgtat
     1261 cttacttagt aactagaatt aaagcagaaa aacatgaaaa gtacataatt aatgtctgta
     1321 gatttatgct tatagcatgt gcagcaatgt gttgttttat agaaccactt acaagctggg
     1381 cattaggtga tatatgtata ggtgtattaa catatataaa tttaattgcc atctttacac
     1441 taaaaaaacc agctataaag gcatattatg attatataag acagaagaag atgggaataa
     1501 aaaggagtga tagaactttt aaccctatag agttagatat aagaaatgct gagttttggg
     1561 aaaatagata cttaaaagag gataagcatg agttaaatta aagttataat aaaatataaa
     1621 aattactaaa taaatttagt gggaggtaag attggtatga aagaaagaat aatacaagtg
     1681 gctgattcaa agaaagaaaa aattctaggg ctatgtcaat ctttatatga tgaaccagaa
     1741 attgctttgc aagaatataa atctgctaaa aaaatatcag aatttctaag agaagagggt
     1801 tttgatgttg aggaaaactt ggcaggtatg gctacagctt ttaaagcaac caagaaaaat
     1861 ggagatggtc ctaaaatagc atttatagca gaatatgatg ctcttccagg caatggacat
     1921 gcatgtggac atcatttaat tgcatctatg ggagttggag ctggaatagc tctttcaagt
     1981 atactggata cttacaaggg agaagtaagt ataattggaa cacctgctga agaaactgga
     2041 gatggaaagc cttacttgat agaacatggt gtatttgatg gctatgatgc agcaatgatg
     2101 atacatccaa attcaaaaac ttgtgtaaca cctgaaatta tagcaatagg aggtctagat
     2161 ttcatattta ctggaaaagc atctcatgca ggagctaagc catataacgg aataaatgct
     2221 ttagatgcag tagtactact atataataat ataaatgcac taagacaaca attagtagat
     2281 ggaacgagaa tacatggtat tatattagaa gcaggaactg ctgcaaatgt aatacctgat
     2341 atgggaaaag taagattaga gataagggca aaagaacaaa attactttga tgaagtagta
     2401 gaaaaagtga aaaattgtgc tagaggtgct gcaatagcca ctggatgtga acttgaattt
     2461 tatcactttg aaccaacttg ccaaggatta aatgaaaata aagttttggt agacatattt
     2521 acaaagatta tggaggaatt tggaatatat gaagatgaac aagtaatgtt aggttcaaca
     2581 gatatgggaa atttaagtca aataatgcca tgtatacatc ctttgatgaa gttttctgaa
     2641 aatggagaag aactacatac aaaagaattt ttggaggctt ctataaattc atatgcaaaa
     2701 gatagagtaa ttgatggtat aaaaatatta gctttaacag gatttaattt atttgaaaat
     2761 ccagaattat tgaaaaaaat gaaggaagag tagaacttag actattttta attatactct
     2821 ttaaaataag aagagtgtct caaaatgaac tttttagttt atgagacact cttttttgta
     2881 tgaaattaaa tatattttta ttattttaaa catgaaaaag aggcttatct gtttttatat
     2941 gacctttttt aacttgtagc acaaaatatc atgaatgatt acaattcgaa tgataaaatc
     3001 gattataaga tatataggag gggtacatat ggaaacttta gaagtaagaa atattgaatt
     3061 gagtggtaca aattatgaga taggatacag actaggagaa ttggttgcta atatgccaga
     3121 gataatagaa ggtcagatta ataaatctaa tctggtaagc aaaaaagaag aaaaagagat
     3181 gatagagttg tttgataaat attgtccagg tcttaatgaa gaattgcaag gttttgctga
     3241 tgcaattcaa gtgaattgta atcaaatttt atattatact atgacttatc ttaaaccagg
     3301 ttgtagtcaa gttgcattag caccagaatt aactgaaaat gggcatgtac tatttgcacg
     3361 taatttcgat ttctcacaca atatggagga ttttgtttta tgtaaaacta aggtaaatgg
     3421 aaaatatgca catataggta ctaccattat gcaatttgga agaggtgaag gtatgaatga
     3481 atgtggctta ggtgtaagtc agtcatcatg tggtattcct gtaggcaatt cagatggttt
     3541 aagaaaacca gcaattgtag gtttacagtt ttgggctgtt attagatatt tgttagaaaa
     3601 ttgcaaagat gtagatgaag cattagaata tttaaaagat atgccaattg catacaatat
     3661 taatttattg cttgcagata agtctggaaa cattgcactt gtggagactt tagatgggaa
     3721 aaaagaagta aatataatca acagtttaga aagtaaaaga gaatattttt tacactctac
     3781 taatcataca catattgata aacttcataa attagatcca caatcaatga aaaattctat
     3841 tcatagatat aaattaatta aagaatatat aaataagtct aaaaaaattg gagagaaaga
     3901 gttaatgaat ttattgtctt caaaatatcc aaatggctta tcttgtaatt actataatga
     3961 tttttttgga actttaaaaa gtatagttat ggatttaaat ataggaaagt ttaatatttt
     4021 atggggtgga ctagagaata aatgggagag ctactattta aaaaatgaca ttaaatctat
     4081 aacacagagg ataaacataa atattgaaaa agcaccatct gacttttttg atttcattga
     4141 ataaaaaatg tatttaatta ttctaaataa ttaggacata taaatcaact aaaagttttt
     4201 atttatcgta ttgaattagt tataatattt tagtatagta aaaaatagtg ctattttaca
     4261 tatttaaaga gtttaagtag aagagaggtg ccatattgga ttacttaaag tattttgaaa
     4321 aagctgtaat gtatattgag aataatttac atgaaaatat aactgtaaat gatgtggcaa
     4381 aagaaactgg atattcttat tatcatttaa cacgcttatt taagtctatg tttggtgaaa
     4441 gtgtaggaag ttatataaaa aaaagacgat tagtaagtag tacaaaagaa cttttatata
     4501 gtgataaaaa agtgatagat atagctattg gtagtggatt tgaatcttca gaagcattta
     4561 gcagagcatt taaatctata tacaaggtta gccctataga atatagaaaa aatagaattg
     4621 atgtatttgt tggtaaaaag aaaaaattag agttagattt tatgagacat cttgttggaa
     4681 atataacgat taaaccagtt ataaaagaaa tagaaaaaat aaaggtaata ggtataaagg
     4741 ataaagtaat tttagaaaat aattcattac ctgacttgtg ggagaaattc agaaaagtac
     4801 atcatattgt cccaaatact ttgccatcaa ggagagtttt tggaatctgt gaagcaatct
     4861 ctgaaataca cctagttagt gaaagtatgg agtttaatga gataatagga cttgaagtta
     4921 atagttatga tgtgattcca aattcatttg tttcaaaaac tattaaggaa ggaaagtatg
     4981 ctatatttac tcatactggt agcttagata atttagacaa aacttatgaa tatatatggg
     5041 gtacttggtt tttaagttca aaagaagaac ttgatataag agatgatttt gaagtatatg
     5101 atgaacgatt tttaggtcct agta
//
LOCUS       DAGSHT010000229         5051 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.235, whole genome shotgun sequence.
ACCESSION   DAGSHT010000229 DAGSHT010000000
VERSION     DAGSHT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.235"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(222..743)
                     /locus_tag="MJB72_003289"
     CDS             complement(222..743)
                     /locus_tag="MJB72_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264352.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            complement(795..1643)
                     /locus_tag="MJB72_003290"
     CDS             complement(795..1643)
                     /locus_tag="MJB72_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBY8264353.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            complement(1793..2074)
                     /locus_tag="MJB72_003291"
     CDS             complement(1793..2074)
                     /locus_tag="MJB72_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264354.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            2459..3313
                     /locus_tag="MJB72_003292"
     CDS             2459..3313
                     /locus_tag="MJB72_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8264355.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            3512..5017
                     /locus_tag="MJB72_003293"
     CDS             3512..5017
                     /locus_tag="MJB72_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8264356.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLTNLNKELDTLEKQKE
                     RLHDFLEQGIYDNNTFGERLKILLSRIENVREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
BASE COUNT         1899 a          655 c          689 g         1808 t
ORIGIN      
        1 acaattttat tatatcaaat gtcatacttt tcatcttaag tgtctgtaat tcaacaatga
       61 acaactttat atttataaat tttacttaca tagtataagt ttcttttaac atcttatata
      121 acaaaaagac agatattcaa ttgaaaatta tctgccttat ttttaatcta tatatcatac
      181 atttatataa aatatatatg tatgtttttt tattattaaa atcaatctct aatatcattt
      241 agcaacttct caaaccattc aatattaact aaatggtgag aacgagaatt ttctagcatt
      301 ctattaaaat aatgtgaatt tgttttgtca ttttctatgg attcatttaa tagagaaatt
      361 cttacttgag tttgcttaat ttgcgtttcc aataattcta ttaacttttc atttttataa
      421 tcatttgcaa ataatagtgt tgcgtaaaat gatagattta tatactctgt tttagaacca
      481 tacttataca ttaatttttc aaattcctgg catccaagtt cagtaattat gtagttatga
      541 gtttctctat ttttattaga ttgtttattt tcaacttttt caataaactt tttttcttca
      601 agttgctgta aattataata aaaagaacct ttagtgaaat ttactatgta cttgtaatgc
      661 ttcttttcca ttaatgctaa taactcatat ccataagaat ttggattttg ttttaataat
      721 cctaaaatta ataatggtat caaaaaatca cctctttcaa tctatattta ttagtatatt
      781 aatgaatatt ttatctaatt atcattaagt ttaataagtt tagattcaaa ctcatcatat
      841 gatatctttc ctagtgccaa gtccatacta tttatatata ttttttctgt ttttgtatat
      901 gttttggaat tactcggtat tatcaactca ctttcattag ctaatgcttc cataatatgc
      961 tttttcttat gaaaagcttc aaaataacgt aatccaaatt ttctttgaga tattgcatca
     1021 atatgaatac catcaggatt agatgtaaga cctgttgctg taacaaagta acaattatct
     1081 tgttcaaaag caaatttctg taactcttga ttgataaata catactcagt acaacttttt
     1141 ccaaaaccta cttttcctaa gaagtctccc aatccaccaa taataattgg tatatctgga
     1201 atatttaatt cttttctaag agtttcaata atagaaagta attttttata ataaaattta
     1261 tagttgccat tattactgtc attttctccc tgatgccata aaattcctgt caattcactg
     1321 ctttgtatgg caaatttagc ttcacttata gcatgtttaa aaagtatacc atctatattc
     1381 cattcatcta aggaactgcc tccctcagca caaggaatta gcccaattct atcctcttga
     1441 ttttcacagc accatgcatc tgcaaatgat gcagcaagac ttaccccaga aactggtctg
     1501 tcataattaa taggttctgt catcatttgc catcttccat tacgtaacat ctgtattctt
     1561 tcattataaa ttggagtcac ttcacttatg aatcctcttc cagccatatt ggactgtcct
     1621 aacattaaaa atgatttaat cattattatt attatccttt cttaaattct tattgtattt
     1681 atttttaata gtagtctaat tagactatct atgtttatat tatagtctaa ttagactata
     1741 agtcaataat attataaaaa atacttgtcc tactttactt attaaagaag tgctaatgac
     1801 tagcaacttc tttgtttgaa ttttttattt gtaattgtct gtctaccatt attttttcta
     1861 ttgctccata tattattgta tctttttctt gtttttcttc taatgtgaac tttcttccta
     1921 agattgtttc aggagatact acatggcaga ttgtctttcc atgtagtatt gtcatagata
     1981 ttttatatcc attctcgtct gttggaacac ctatatcgtc gttgaggtat gttatagtag
     2041 ttgtaccatc taagtctttt tctatgtttg gcatttaaat cccctcctct ttatatttta
     2101 tgtatcttaa aaattgtcct attctcaatc attatttata attgaataac taagtgttgc
     2161 ttgattaagg gtaaacttca aaaatagtta actataaata aaatgttata tataattaaa
     2221 tgtctaagct aaaatattat aaaaatttca taataaatta aaactgttat cttatataaa
     2281 aattagatac taatttgtga tatactttaa tgtgaaaaac aatactattt gttcattttt
     2341 gtagttaatt acagactttc tcatgttcat tctttattaa gctagttaat tagtgcatag
     2401 aaagagagga ttaactattt taaaatagat attatataaa ttacaaggag ctgattttat
     2461 ggaatggatt gaaagattaa acaaagcaat taactatatt gaagaaaaca taacaaaaga
     2521 aattgagtat gaacaagtgg caaaaatagc ttgttgttct acatatcatt ttcaaagaat
     2581 gtttgcttat atggcagatg taccactttc tgaatatatt cgtcgtagac ggatgtcttt
     2641 agcagctgta gaattacaaa atgatgataa aaagataatt gatgtggctt taaaatatgg
     2701 atactcttct ccaacagcat tcaatcgtgc atttaaaagt attcatggtg ttgccccatc
     2761 agttataaaa aaaagagaaa taacaacttt aaaagccttt cctccaatca gttttaaaat
     2821 tagtattaaa ggagccgaag aaatgaatta tagaattgaa aagaaagaag catttcgtat
     2881 agttggtgta tctcagccac ttcataatga actagaaaag aattttgaaa ttgttcccca
     2941 aatgtggcaa aaggttgctt tagatggaac gcttcaaaaa ttaatcccta tgatggatag
     3001 tcaacttcaa ggtgtattag gtataagtat ttgtaataat tcagaagaat ggaagtattt
     3061 tatatctgtt tcaagtacaa aatctataga taatacatta gaagaataca ctgttccagc
     3121 ttttacttgg gcaatatttt ctggagaagg acaatgtcca caagcaatac aagaactaga
     3181 aaagcgtatt gtaacagaat ggttacctac ttctggttat gaatatgata atggtcctga
     3241 tattgaactg tacttaaatc cagaccctca aaatgctaaa tttgaagtat ggatacctgt
     3301 agtaaaaaaa tagtttaaaa ttaaaaaatg aagaaagcat aaaattaata gtataaatat
     3361 ttacttagat tacaagcttt taaatatact ataaaattta ctgtataaaa ataaacctaa
     3421 aaaatatatc ttgtattttg aaggtttatt tttgtgtttt aaatataatc aatctgttac
     3481 tgaatataat atattcaagg ggtgtgattt aatgaatgtg gcaatttatt tacgcaaaag
     3541 cagagctgat gaagaagcag aacatagagg tgaatttgaa actctaagta gacataagac
     3601 aactctttta aaactagcaa gagaacaaaa tttaaatgtc attgaaatta aagaagaact
     3661 tgtttctggg gaaagtatag aatatagacc taagatgcta gaattactag gtgaaattaa
     3721 aaataataac tatgatgctg ttttagtgat ggatattgac cgtttgggtc gtggtaatat
     3781 gcaagaccaa gggttaatac tagatacatt taaaaaatct aaaacgaaaa ttattactcc
     3841 tagaaaaacg tatgatttaa atgatgagtt tgatgaggag tattctgaat ttgaagcttt
     3901 tatggctcgt aaggaattaa aattaatatc tagacgtatg caaagaggaa aaatcaaaag
     3961 tattgaagag ggtaaattta tagctcaagc tcctccttat gggtatgata ttaaatattt
     4021 aaataataaa gaaagagtac ttgtaataaa tgaagataaa gcaactgttg ttaaaataat
     4081 ctttgatatg tatttaaaag gagatggtgc atgtaaaata tccaattatt taaacgaact
     4141 tggttataag accacaacag gtagaacttg gtatgaaaag ggtataagag atattataaa
     4201 aaacaaagtg tattgtggat atatagtatg gaataaagtc gagaggaaaa gaaattctag
     4261 tagaacaaga ggaattgagg aacaaattga ggtaaaagga aagcatgaac caataataag
     4321 tgaagacata tggaataagg cacaaaatat aagaaaaaaa cgtattaatt cccctactcc
     4381 aaacaataaa ataacaaatc cattggctgg aattgtaaaa tgtggatact gtaatcatac
     4441 tttaatagcc aaatctaata ctacatcaaa tggatatgag aaatatatgg tttgtactaa
     4501 ttgtaaaaat aaagctacta aactatacat attagaaaat aaaatattag aggtcttaaa
     4561 taaatggtta gtaaattata gactatcttt aaaagatgaa aaagataact ctaaacctga
     4621 aaatacttta gaacatacct tgacaaattt aaataaagaa ttggatactt tagaaaaaca
     4681 aaaagaacga ctacatgatt ttttagaaca aggaatttat gacaataata catttgggga
     4741 acgtttaaaa atactcttat ctcgtataga aaatgtaaga gaagctatta ataaaacaaa
     4801 tatagatatt gaaatgcaaa aggaaagaaa tacagttaaa acacaaattg ttcctcaact
     4861 tgaaaaaatt ttaagtgaat atacagagga aaaagaacct tatctaaaaa atatattgct
     4921 taaaagtgta ataaattatg cagtctataa aaaagaacct aatcaaaaat ctgatgattt
     4981 ttctttaata ctttacccta atatcccaag agattaatta tatgttttaa atgttataaa
     5041 tagactgatt a
//
LOCUS       DAGSHT010000230         5001 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.236, whole genome shotgun sequence.
ACCESSION   DAGSHT010000230 DAGSHT010000000
VERSION     DAGSHT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.236"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            114..1973
                     /locus_tag="MJB72_003294"
     CDS             114..1973
                     /locus_tag="MJB72_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264357.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2265..2864)
                     /locus_tag="MJB72_003295"
     CDS             complement(2265..2864)
                     /locus_tag="MJB72_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY8264358.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2967..3764)
                     /locus_tag="MJB72_003296"
     CDS             complement(2967..3764)
                     /locus_tag="MJB72_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY8264359.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3877..3983)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4075..>5001)
                     /locus_tag="MJB72_003297"
     CDS             complement(4075..>5001)
                     /locus_tag="MJB72_003297"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBY8264360.1"
                     /translation="LLKNFDEDLFILWRNEPSIIVGKNQNTLSEINLEYIKEYSIPVV
                     RRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPIIDLLKTLDVNAEFSGRNDLL
                     IDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALKVKPIKFEGKGIKSVKSRVTN
                     ISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHDIKQIEKLTKSKYETWDWNFG
                     NSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDFFGKCDVAFVENKLIGIRHEE
                     NALRNTLDSIEINDYFLGADTDILVSGILGVK"
BASE COUNT         1822 a          644 c          691 g         1844 t
ORIGIN      
        1 atatattcta attgtataga aaaatttatt gtaaacgcat gtatattatt agaaaaaata
       61 gtttggaggt attcaataaa cataaaaaac aatatagggg ataaaggtgt tttatgaaga
      121 gatttttgag gagaattatc ttagttttat ttattttgtt gatttttata agttttattt
      181 ctattaaatt aattcataat gttggagatt atggaaaatt gattaattat gttgggattg
      241 ttcgtggagc tagccaacgt ctaactaaac tagagatgaa tcatcaacca aatgatgaat
      301 taatagaata tattgatgaa atcctacagg agttgattac agggcatgga gattatggtt
      361 tagtaatcac tgattgtaat gaatacaatg aggacttact tttattagaa aaaaagtggg
      421 aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga acagaataac cagttattaa
      481 gtataagtga agagtttttt tctcttgcaa atgatacagt gtttaaaata gaaaactttt
      541 ctaaagaaaa aagtaattac ttaatgactt taataataat tatatctatt ataggtatat
      601 tagcatgtat catattgatt ttacaatata gtaaaaaaat ggttagactg gaaaaattaa
      661 atgtagattt aaaaaatatt gcatataaag atgaactaac aggagtaaat actatagaaa
      721 aatttaaatt ggatgcaaat caaaatattt gtatacataa ggataagaaa tttgctgtat
      781 tctatattga ttttgaaaac tttaaataca ttaatgatat atttggatat gattatggtg
      841 acatgatact gaaaagatat gctaatctta tgatgaatga catagggaaa tatgaaatat
      901 ttgcaagaga aattgcagat agatttgttg cgttaagatg ctatgtagat aaggaagatt
      961 tagtggtaag acaacgagat gtagacaatg aactaattaa tataacaaat gaaattaaaa
     1021 ataaacatag tattactgtt gttagtggaa tttgttgtat tgaagatgta aatgaaaaat
     1081 taagtataga tggtttaata aatagagcaa attttgcaca aaaaactgtt aagaataaac
     1141 caggtactaa ttatgctttt tataatgaca gtattcgtaa aaaaatgatt gaagaaaata
     1201 ctatcaaaag tagaatacat gaagctattg aaaaaagaga atttatagtt tatttacagc
     1261 ctaaagttaa tttacatagt caaaaaataa attgtgcaga agctttagtt cgttggctta
     1321 ctccggataa aggtattata tctccagcaa tatttattcc agtacttgag aagaattttt
     1381 ttattgcctt agtagataag tatgtttttg aggaggtttg taaatggatt agaaagaggt
     1441 tagatgaaaa taaacctttt gttcagatat cagttaatgt ttcaagaata caattttata
     1501 acactaaatt tgtagaaact tattccaata tacaaaataa atacaggata cctaaaaata
     1561 ctatagaaat tgaatttaca gagagtgttg cttttgagaa tcaaaaacat cttttggaaa
     1621 ttatacatga tttgcatgaa aatggattta cttgttcttt agatgatttt ggaaaaggtt
     1681 attcgtcttt aagtgtttta aaagatttac catttgatgt tttaaagctt gattctatgt
     1741 ttttcaaggc tagtttagat aaagataaag agaaaatagt aataaaaaat attgtacata
     1801 tgttaaaaga acttaatatc acaactgttg ctgaaggcat tgaatgcaag gaacaagttg
     1861 aatttcttag ggatattggg tgtgatttag ttcagggatt tgtattttat aaaccaatgc
     1921 caatattaga atttgaaaaa atattggata aagagtttgt ttataatagc taaaatgtac
     1981 tttataaaat atgaactaat taagtaaaat tattttgtac tgaataaatt tttactttat
     2041 ttacagaata atgattattt acagaaaagc gcgaaaagtg tattccttta agtgtgggat
     2101 ggatagcaca aataggggag aaaaaatatt ggccatataa gaatgcataa taatataaat
     2161 atttgaaagg tcagcattat ataaaaatta aaaagaaaat aaataatttt tctataaaaa
     2221 agagactatg agaaaattta ctcatagtct cctaaataat gtatttattt tcttcctgca
     2281 acccatttta atccccaatg aaattcagta tccatttctc tatgaccttt gaagtattta
     2341 actacttttt caacactgaa tgtctcatca tttacatttt ctatcaatgc tattgaacac
     2401 atattatagc catttttatg ttcatcaagt ttgacaacta agtctgaatc ttgtttttgt
     2461 ttgattgtta caattccatc agcttcagac cagttagcta caccttcgta tataaaagca
     2521 taaataagta ttctttttat atcttttatt ttattaccat ttattcttaa attttctcct
     2581 tgtgtattac ttcctgttct gtcatctcca tctaattgag caaaaggtgg attgtctaaa
     2641 ctaccaaagg catttcctaa tgcttgaaca gcacctttta caccattttt catctcaaat
     2701 aggcaaccca aatctaaatc tatattactg cttcttaaag agccaaaaaa accctttttt
     2761 tgtacttttt gattccaatt taaattaact aagatttcac ctaaatcgct attttccttt
     2821 tttgtaaggt ttattttttg tccctttttt aattctatag ccatagtaat cttcctccac
     2881 aaacatagat ttatttaaat ggtagcactt aataagtcaa tattcaacaa gattagaata
     2941 ataatattaa aattatatta aatatactaa aaagtaggaa tcacaatacc tttgtatttt
     3001 tcttcaatta agcttcttgc tttatctgat gtaagaacat cttgtagaac tttaatcttt
     3061 tcactgtctt tatcttcagt tctaaatgct aaaagatttg catactcgtc atttatagga
     3121 gctgtataaa ttccgttttt cttggcatca agaccagcag gtacagcata agtagtatct
     3181 ataaatgcag cagtcacatc tggaagagaa gatggaattt gagcaatatc taactctttg
     3241 aattgtattc cctttggatt ttctattatg tccttaggac taactaatcc atccccttta
     3301 ggtaacgtta aaagtccaat actttcaagg tatctaaggt tcttagaaca agaagatggg
     3361 ttattgctta ttgcaattgt atctcctttt ttaaattcat caacagattt tatcttataa
     3421 gagtaaagta ttgctggaca tacatatagt tttttaccag ccattatttt gtaacctttg
     3481 gccttaacag cttcttttag ataaggttca tgttgaaata gatttgcatc aatttcacca
     3541 ttatttaaag cttcatttgg aagtatgtaa tcatcaaagt ttttaacttc taaagtatat
     3601 cctttttctt taataagtgg ttttaattct tctaataact ctcctcctgg aactaaagta
     3661 gcaccaacaa caattttttt atcatcttta gctttagaac atccaaccac agctaaagtt
     3721 aatactagac ataataacaa acataataat tttttgaatt tcatataaat tccctcctga
     3781 atataattga ttagttaaaa ataaaatacc attctttatc acactgcaaa ataaaaataa
     3841 gcctgagttt aaactcaggc tttaaaagct aatttagact catctttcag tataatactg
     3901 caggatttag caccaatgta tatagaaaat atactggttg ctgggtttca tagggccagt
     3961 cccttcacca ctctggataa gaagtatcaa tttttaatta tgttataaat attatcatga
     4021 ttttttacgt ctgtaaacat aattttataa atattttaaa atctttaatg tattttattt
     4081 aacacctaaa attccagaaa ctagaatatc agtatcagca cctaagaaat agtcatttat
     4141 ttcaatagaa tccagagtat ttctcaaagc attttcttca tgccttatac caataagttt
     4201 attttctaca aatgcaacat cacattttcc aaaaaaatct ccaaaaaatt ttatactagt
     4261 tattatgcct ttatcaacat ttaaattaaa ctcaacattt ccaccagaat attttaattc
     4321 atttgaaaga gtatattttg gtgaattacc aaaattccaa tcccaagttt cgtattttga
     4381 tttagtaagc ttttctattt gttttatatc atgctcactc aaacaataat tagtattatc
     4441 agtatttgtt ttagaaagat aatccattat agcatcttta aattctaata tatccatttt
     4501 tgaatgtaaa tgttcactta tattggttac acgagatttt acagatttta ttccttttcc
     4561 ttcaaatttt ataggtttaa cctttaatgc ttttgagagg tcatttattt cagatgaaaa
     4621 taaaagagtc ccatgatgca ttactttgtt tttgtagttg tattgagcat ttccagaaaa
     4681 tttcttgcca tctataagaa ggtcatttct tccagaaaat tcggcattga catctagtgt
     4741 tttaagtaaa tctattatgg gctgagtgaa tcttctgaaa tcactaaaat tattattgtt
     4801 acaagctata aatgtaaaat ttatatttcc caaatcatga aatacagctc caccaccaga
     4861 ctgtctccta acgacaggta tagaatattc ttttatatat tctaaattta tttcagaaag
     4921 tgtgttttgg ttttttccaa caattataga aggttcattc ctccataaaa taaataagtc
     4981 ttcatcaaaa ttttttagaa g
//
LOCUS       DAGSHT010000231         4925 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.237, whole genome shotgun sequence.
ACCESSION   DAGSHT010000231 DAGSHT010000000
VERSION     DAGSHT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.237"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..364
                     /locus_tag="MJB72_003298"
     CDS             <1..364
                     /locus_tag="MJB72_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY8264361.1"
                     /translation="IHSMRKLLNEYPFYNKIIKILICELEENIKESEEFKDLKDSFII
                     SIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMLENKLDKADFMLKKALSL
                     DVENEDTIYNIEYLKSLR"
     gene            388..2265
                     /locus_tag="MJB72_003299"
     CDS             388..2265
                     /locus_tag="MJB72_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY8264362.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKRQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSSINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYKKDFFDYIILGDILEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            2258..4372
                     /locus_tag="MJB72_003300"
     CDS             2258..4372
                     /locus_tag="MJB72_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HBY8264363.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINNLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDCLKRAMALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            4381..>4925
                     /locus_tag="MJB72_003301"
     CDS             4381..>4925
                     /locus_tag="MJB72_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264364.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNL"
BASE COUNT         2001 a          421 c          869 g         1634 t
ORIGIN      
        1 tatacatagt atgaggaaat tactaaatga gtatcctttt tataataaaa taataaaaat
       61 acttatatgt gaattagaag aaaatataaa agaaagtgaa gaatttaagg atttaaaaga
      121 tagttttatt attagtatag aaaatatgat tgaaactggc aaaataaatg atgcgaaatt
      181 attggtagat gactattcaa aaacatttgt ggatgatata aagattttaa atataaaggg
      241 aattttatac atgcttgaaa ataagcttga taaagctgat tttatgttaa aaaaggcatt
      301 atcattagat gtagaaaatg aagatactat atataatata gaatacttaa aaagcttaag
      361 ataagtcaaa aataggagaa ttgaagaatg aatataccaa tagtggttaa tgaaaaatct
      421 aaagatataa taaaagattg gcaatatacg tgtacaaacg aagatataaa atttacaagc
      481 ataataatat tgacatataa tcaaatagaa tatactaaat tatgtattga aagtattagg
      541 aaatttaccc caaagaataa atatgaaata ataataattg acaataactc ttctgatgga
      601 acagttgagt ggttaaagag acaatctgat ataaaatctg tgtataataa caaaaactta
      661 ggatttccaa aaggatgcaa tcaaggtatt gaattagcta ctggtgaaaa catattgttg
      721 ttaaataatg atgtaatagt tactccaaat tggctctata atttgaataa agctttatgg
      781 tctcaagata atataggtgc tgtaggacct attacaaatg catgttcata ttatcagcaa
      841 attgatgttg attataagtc aattgaggac atgtttatat ttgcacacaa aataaataaa
      901 tctagtatta acctatggaa atatagatta aagttagttg gattttgcat gttaataaaa
      961 aaaactgttc tggataatgt aggggtttta gatgaattat tcacacctgg aaattttgaa
     1021 gatgatgatt tatcttttag gatgatagag tcaggatata agttattatt atgccaggat
     1081 acttttatac accattttgg aagtgtgtct tttaaagaaa atcgtaataa atatacggat
     1141 ataatgtata ttaatagaag aaaatttgaa actaagtggg gatttaatcc agaatatagt
     1201 ggaatgataa gaacagattt gatttctaaa atagatgata gtttggaggc aaatatcaat
     1261 gttttagaaa taggctgtgg tataggggct actcttttag aaataaaaaa tatatataaa
     1321 aatgcatcaa tatatggaat agaaattagt gagtcagcag gtaaacttgc aaagtatagt
     1381 gcagatgtac ttatagcaga tatagaaaaa gtaaaattgg attataaaaa agattttttt
     1441 gattatataa tattgggaga tatacttgaa catttgattg atccatggaa gatagtcaat
     1501 gaattgaaac aatatctaaa aaaggatggt tatctggtat ctagtatacc aaatattatg
     1561 catgtttctg ttttaagaaa tcttgtgtta ggtaatttta catatgaaga ttcaggaatt
     1621 ttggacaaga ctcacttaag gttttttaca ttaaatgaga taacaaaaat gtttaatgat
     1681 agtaattaca taataaatag tattacagct actagtatgc cactttcgga aattgataat
     1741 agcttcattg atacaatatg taaggaaagc tgtaaagaaa tgagacaaca gtacgaatct
     1801 tatcaatata tagtaaaagc ctctaaaatc ctagatacta aaagatattc tagtattagt
     1861 atggttaatt taaaatacgc tttaatgaga attgataatg aattagatgt agatagtaat
     1921 ttaagttata tatttgataa ttataatata actgaaccat gttttgttga tgatattgaa
     1981 tatctaattg atacgtttgt gataaataaa gcaactctat taaatagatt aggagttgag
     2041 gcatttaaca gaggtctagg agattttagt ataaacatgt ttatgacagt attagagatg
     2101 gatagagatg atattgactc aatatataat ataacaagtg tattatgtga attagaagaa
     2161 tttgatactg cttataattt aattaaaaat agtagcgaaa aagtacagag tgaaagtgat
     2221 atacaagaga tactttgttt tattgagggg aagaaatatg aataatgata agatatgttt
     2281 tataacctgt gtaaatgatg aaaggcttta tgaagaatgt cttgtatata taaataacct
     2341 agacattcct aaaaacttta gtatagaaac agtagcttta agaggagccg attctattgc
     2401 ctctgcttat aataaagcta tgagagaaac agattctaag tataaggtat acttacatca
     2461 agatactttt ataattaata aaaatttcat acatgatatt ttagagatat ttaaaaaaaa
     2521 tacagatgtc ggaatgattg gtgtagctgg agtaaaagat atgcctgtga atggaatttg
     2581 gtgggattct gataaaaaag cagggaaagt atatgatagt catacaggta aaatggatat
     2641 ttatgaattt agtaactttg aagaatgttt taatgaagtt atggctatag atggattaat
     2701 tatgattact caatatgatg tattttggag agaggataag tttgatgggt ggcattttta
     2761 tgatttgtca cagagtatgg agtttagaca aaataaatac aaagttattg taccaaaaca
     2821 aaataagcct tggtgtatgc atgattctgg agtagtaaat ataaataatg gatatgatga
     2881 atataggata aaatttgtaa aagagtacat aaatcttgat ttatatagag aaaattttta
     2941 tgaatttgat aagaagaaag caagtaccat acttaagagt aataagcatg taatggattg
     3001 tatgaaagag gagcttacag aacaattaag attaaagaat tacaaggatg ttttaggtat
     3061 tgtttctaaa attgctcatt tttctgtttt taatcatact ggtttgtatt cttgtgaaga
     3121 atgtgagaga gcattgctag aatgtgcaaa taatttacca gaaatagaaa atacgaaatt
     3181 atttacaaaa aaagatttaa aaagaagaaa agtattacat gttttaagtg aaggatattc
     3241 aacaggaggt catacaagac tagttaaaaa ttggattgaa attgacaaag actctgttca
     3301 ttcagttgta accacatggc aatcagatac actgccagac tggttattaa aagcaataga
     3361 tgatagtggt ggcgaagtta tatctttgga tttaatttca gatgattgtt taaaaagagc
     3421 tatggctttg agaaaaatag cttatgactg ggctgatata gttattttat atgttcatat
     3481 gtgggataca atacctgttg ttgcttttgg agttgatgga aaaataccta ttttacatgt
     3541 taatcactat gacagtactt tttggttagg gacaagtatt gcagatattg ttattgattt
     3601 tagcgaagct agtcaattaa ttacattaga taaaagaggc atagataaat ctgaaatatt
     3661 accaatgcca ttaaaagaaa aagaagtatt ttcaaaatct gaagttagaa aacgatataa
     3721 tttggaggat gatgtgacaa taatattgac tattgctagt gaaagaaaat acaatagtgt
     3781 aaatgatatt aattacttta aaattgctaa aacattgatt gaaaatacag ataatacaat
     3841 tattattgtt gtaggagttg atacagagaa tctttattgg aaaactttaa gtaacgttac
     3901 agataataga gttatatgtt ttggaacaac agcaaatata gacgattttt acaagatttc
     3961 ggatatatat atggagtctt tcatggtagg aagtcatact tcaaaactag atgcaatttt
     4021 atatgattta attcctgtta agtttaaaaa tgaagtttca ccaattttta caaatatgtg
     4081 gaattttata gataagtttg attataagaa tataaaagag gttatagata tgataaaaag
     4141 gtacaatact agtgaatttg acagggatag gataaaagaa aaacaatcgg aaataaaaca
     4201 ttttgttata gataaacact gtattgaaat aaaaaactat atagacaaaa tatataatga
     4261 agctaatcat cataaagtta agtatgattt atataaaaat gatgaaatcg aggactatca
     4321 gctttttgta gcattatatg gctatatggt aaaaaataaa aacatgtcat aggtggtaaa
     4381 atgaacaata ttcatatcaa taaatttgaa aaaagctgtg aaagaaactg ggattatttt
     4441 gtagaaaata aatctataaa tggtacattt ctgcaaacta gaaatttcct taattatcat
     4501 gaggagggaa aattcactga ccactcatta ataatatgta aaggtgaaga aatactagca
     4561 gtcgtacctg catgtgaaac ttatgaagat ggtgaaagaa tatttttttc tcatttaggg
     4621 agtacttttg gtggtattat aataagaagt ggttttaatg atattaggca tgtaaatgcc
     4681 attacaaata agttagaaat atatttacgg gaacaaaatt tcgataaagt atatttaaaa
     4741 aatacatcaa gaatattttg taaagaggat acttcattac tagaatattt tttatttaag
     4801 aataattaca tgttttatga tgaattaagt atgtttttag aattaagtaa gtataaagaa
     4861 gatatattag ccaattttaa atatagtaaa agaagagatt ttaaatattc tttgaagaat
     4921 aatct
//
LOCUS       DAGSHT010000232         4916 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.238, whole genome shotgun sequence.
ACCESSION   DAGSHT010000232 DAGSHT010000000
VERSION     DAGSHT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.238"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(503..1144)
                     /locus_tag="MJB72_003302"
     CDS             complement(503..1144)
                     /locus_tag="MJB72_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBY8264365.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1387..2190)
                     /locus_tag="MJB72_003303"
     CDS             complement(1387..2190)
                     /locus_tag="MJB72_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY8264366.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2346..3521)
                     /gene="thiI"
                     /locus_tag="MJB72_003304"
     CDS             complement(2346..3521)
                     /gene="thiI"
                     /locus_tag="MJB72_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBY8264367.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3567..4718)
                     /locus_tag="MJB72_003305"
     CDS             complement(3567..4718)
                     /locus_tag="MJB72_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBY8264368.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1530 a          935 c          468 g         1983 t
ORIGIN      
        1 ttaaatttat gaacattttt tgtttttatg ttaggatatt tcttagaaat gaaatacttc
       61 ttaatttgat ttgatatgta tatttttata gctattttat tataaaaaaa aagaaccctt
      121 ttataactaa gggttacttt ttttctctat tttttcttat tatatcacta ttttttccct
      181 atttcaatat tttgacattt tttccattat actgaaaaga tatcttaaat ttttaacttg
      241 gataaatttt aaagcatata cttaaaatta acatacttgt tttctatttt attacactta
      301 atccttattt ttatcattct acaaaacaaa aataaaaaag aataagataa taaataaaat
      361 acttatcacc ttattcttta tttaattatt ttacaacgaa aaatctaaac tactttttaa
      421 atagcttcct cttccatcaa tttatttgta tattactggt ttaattcttc aagtaactga
      481 ttggttcttg caatacttta tcctaaactc tggaaacttt gtaaactacc tctctagcat
      541 ttggtaaatt tccatctgga atacctacag caataccaaa tggtacagaa tacccttctg
      601 gtatatcaag tagttctctg tattttttat ttgtatcaaa cttcatcttt attgactctg
      661 attcttttac atcttcttta tccaatacac tcacaagacc ttcaatatcc ccttgttcaa
      721 caggtgtaaa taaacctcta accattccta ttatacaact tccaagccct agtgcttgtg
      781 cagctgttgt tatattccct gtaagtattc ctatatcata tggagcccat gaactacttt
      841 catcataaga aataaataaa acggttggag caccataaaa taacttaaag tttttattta
      901 tctcaacatt actcacctca tgaatcctag ataaagtatc atcactaatc tcttttaaaa
      961 gtttctggtc ttgcacaaca gttatgtgcc aaggctgttt attacatcct gaaggtgctg
     1021 caaatgcaca gtgtaataat gtgtttactt gctcatcact aatttgttct gtcgtaaatt
     1081 ttcgtatact tctacgactt tgtattaaat ttattgtttg gttgtcttga aaattattat
     1141 tcatatctat tccccttttt atgtatttct tagtacaaat actaaatctt cagaaaatta
     1201 atcttataat taaatttctt tacaatatta ttataattat ttttagcttg tatgtgaaga
     1261 cttaaaaata taaacacttt taatatatct caattatata tataaatgaa aatctggtat
     1321 tcgtcatcta tagaaactgc tcttttctat agtttaatca ataccagatt aataaaacat
     1381 gatatattaa tttaaaggac cccatccctc tttagaatga atctctgttt ttacagactt
     1441 aacactatct gtgtcaacat cccatgacct acctgcaaca gaaataccat ttttaccttt
     1501 ataccatcta ccttcatcat aatcgtaaac tagggaagta taccaattac ccccagtctt
     1561 attatttgca aatacaacta cagtagcatc agatttagta ggactatatg catatggttg
     1621 tatatatagt gtatccttac ttaatccata ttttgcctta aattcatctg gaattgtagg
     1681 ccacccattt ttatatttat tgtctaataa atattttcta aaatcatcat taccctttga
     1741 agctcctggt attatagaag ggtcttgtgc aaatgatgca atcaaatctt tcatactttg
     1801 atgtatatcc ctagccattt caacaccgtc tggatgtttt gtacaagtaa catacacttt
     1861 tgcaatgctt tctattccag ttattccatc ataaccatca tcaaacgttg ctgaatatat
     1921 tccacctgac ttacactttg gttctgtttc aaagtactta ccatctttgt tttctaatac
     1981 ctctttcatg acttcattct tgcctttact tgattcctca gccattgcaa taacaatttg
     2041 agtctttata ttttctctat tagaaagaca tgtaactgct ttactttttt ctatatttct
     2101 aaacaatgct ggaacagcta ctataactaa aatgcctatt atagatataa ctactaacaa
     2161 ttcaattagt gtaaaaccct ttttattcat aataatcatt cccattaaat aaaattatag
     2221 tacttatttt taaatactac atattaataa attgtctaca taaatccttt caacagaact
     2281 actaatatac ttacagaact actaatatac tcaatattca atttagtaat taaataactt
     2341 gtatgctaaa actcaatatc ctctacctct attttgtcaa tagcatcctg tactaatttt
     2401 tcaatgtcaa gtgctgtttc agatttttct attttctcta atctaggttt agtgacgggc
     2461 tttctaggag aaaatacact acaacaatct tcttcaggca atatagatgt ttcaaaagtc
     2521 cctatttttt tggctatatc aactatatct gatttatcca ttgctataag tggtctaaac
     2581 actggtaagt ctacaacggc attagtacaa gtaagtcctt gtatagtttg agatgcaacc
     2641 tgcccaatac tttcacctgt aattaaagca tcacaatgtc ttttttcaga aagtctctgt
     2701 gctatcctca tcataaatct tcttgaaaga atagtagctt cttcttcatt acaattctcg
     2761 ccaattgctt tttgtatttc taagatatta actttatgaa gccttactct accacagtat
     2821 tttgccagta ttttagctaa atccttaact ttttcctgtg acctttcact tgtaaatgga
     2881 taactatgaa agtgaactgc ctctacttcc atacctcttt ttgcaaccat ccatgtagct
     2941 actgggctat ctatacctcc tgataaaaga gacatagctc taccattcgt tccaagtggc
     3001 aatccaccat atccaggtat agtatcacta tagaccatag tatgaaattc tctgtattcg
     3061 cactttattt taacttctgg atttcttaca tcaacattaa tcctatctcc tacctttgat
     3121 aataagtatc cacctatatc catactcatt tcttgagaag tcaacccgaa agacttatct
     3181 cctcttcttg actctacctt aaaagtttta tatccagcct ctattttttc ttctaacatt
     3241 tttaaagcta tttcttttaa tgtatcataa tcttttttag ctcttactcc agggcacaca
     3301 cccactatcc caaatacttt tctaacttct tcaattactt cttcataatc atagtcttct
     3361 aaatcaacat atattcttcc atactcttta tatacattaa atcttccaat tggttttaac
     3421 atatttctta tatttcttat aagcctattc tcaaatatat atctattctt tccttttact
     3481 ccaatttctc catactttac tattaatata ttatacacca tttcacctct taaactaatt
     3541 tattatcact atctatacag tattctttat tttcttttca ttattgacct taagtcacaa
     3601 attgattctt tcaaaacttt cacagcttct aacatttctt cttctgtatt catatcagat
     3661 aaactaaatc ttattgttcc atttatttct tcatttgtaa gacctatagc atttaatacg
     3721 taactacctt tctttttaga agaacatgca gagccagttg atacataaat acctttttgc
     3781 tctaagtaat gaagtaaaac ttctcctttt gtccctctaa atgatacatt taatatatga
     3841 catactccat cttctggaga attaattttt atatcttcta tattatcaat tatttcattt
     3901 tttaatatat tttttaagtt atttactttc gaaataacag catctaaatc tttgtttagt
     3961 attctaacag cttctccaag accatatatt ccaggtacat tttctgttcc cgacctaatt
     4021 ccaatttcct gaccaccacc tgttaacata ggcttaaggc gattattttc ttttatatac
     4081 ataaatccaa ttccctttgg accatgaaat ttatgtgcac ttacactcat aaaatctata
     4141 ttgtatttag atggtctaaa gttaattttt ccatatgact gaatagcatc aacatggaaa
     4201 taaatttttt cttttaaacc cttcaaatac tttccaactt cacctattgg ctgtatagtt
     4261 cctatttcat tgttaacatg catcatgctt actaagcatg tagttgactt tatcgcattt
     4321 tttaaatcct ctatatttat tttcccatct tttccgacct ctaaataagt tacttcaaaa
     4381 ccatcttctt ctaaatcttt taatgtattt aaaacagaag gatgttctat gttagtagat
     4441 attatgtgat tttttctttt tttatttaaa ctagtaatac ctcttattat agtattatta
     4501 cactctgtac cacctgatgt aaaataaatc tccttttctt tcgctcctag agatttagct
     4561 atatcttgtc ttacttcttt tatagctttt tcaacttcta caccttttct atggacagaa
     4621 gaaggatttc catattccgt attaagtgca taaaccattt tatctataac ttcttgataa
     4681 ggttttgtag ttgcgctatt gtctaagtat atttccaact taattacacc tacctttatt
     4741 taaaatcttc aactctaact attataccac taattatagt attttttttg ttaatcttta
     4801 ttaaaaagac aatcttaaat tataaaatat tatttcatga aaagctagtt ttttcacttt
     4861 attacaaatt tttatagtaa aatataatat acttatacta taccctacaa aaatat
//
LOCUS       DAGSHT010000233         4915 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.239, whole genome shotgun sequence.
ACCESSION   DAGSHT010000233 DAGSHT010000000
VERSION     DAGSHT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.239"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..654)
                     /locus_tag="MJB72_003306"
     CDS             complement(<1..654)
                     /locus_tag="MJB72_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8264369.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     "
     gene            complement(850..1050)
                     /locus_tag="MJB72_003307"
     CDS             complement(850..1050)
                     /locus_tag="MJB72_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264370.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(1175..1555)
                     /locus_tag="MJB72_003308"
     CDS             complement(1175..1555)
                     /locus_tag="MJB72_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY8264371.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEIGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINASHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(1828..3186)
                     /locus_tag="MJB72_003309"
     CDS             complement(1828..3186)
                     /locus_tag="MJB72_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8264372.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3634..4086
                     /locus_tag="MJB72_003310"
     CDS             3634..4086
                     /locus_tag="MJB72_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBY8264373.1"
                     /translation="MVNLNSNNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(4243..4860)
                     /locus_tag="MJB72_003311"
     CDS             complement(4243..4860)
                     /locus_tag="MJB72_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBY8264374.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
BASE COUNT         1614 a          864 c          487 g         1950 t
ORIGIN      
        1 tccatggctt tttactgaac catcttgtag aacaattatt tcatcagcta actctaatat
       61 tctttcttgg tgagaaatta tgattatagt ctgattagac tcttcatgta ttttcttaaa
      121 actttcattg agttttccaa agctccaaag gtctatccct gcttcaggtt catcaaatat
      181 agaaaccttt aaatctctag caaaaagagt tgctatctca attcttttca tttcccctcc
      241 agaaagagag ttatcaactt cacgatttaa atagtctttt gaacacaatc caacatctgt
      301 aaggtattgg caacatacat cagtagacaa tggttttcca tgagcaagtg taagtaaatt
      361 ttcaactgtc atacctttta ttcttggtgg ttgttgaaat gcgtatccta ttcctttttt
      421 tgctctttct gtaatactaa gatttgttat atcttctcca tctaaaatta tctgaccact
      481 cgtagctttt tcaattccca taataagctt agcaattgta gattttcctc caccatttgg
      541 acctgtgata acaataagtt ttcccctctc aaatgaaagg ctaacatcat ttattattcc
      601 tagttcctca ttgttagact caacattaaa ggaaagattt tttatttcaa gcataatacc
      661 cctcttttct ataaaatatt acattaatat ccattctttt attatatcag taatttttat
      721 atatcagcaa ctctaaggag ataaaatgta tattaaataa taaaacaaag ttattgaaaa
      781 gtaaaaaaga tgagttttat gtaaactcat ctcagaaatt tagtatatca acttatatgt
      841 atttaaaatt taaccttcta tatactctaa tattattttt tgattatctt ttaacataat
      901 ttcttctttt ttagattcta ttccattctt ttttatcttt acctcagcta caaaggcatc
      961 attaccttca accatttcta atagttcata tgctttttta cgacctattt tagttgctat
     1021 atcttttagt actgccattt tcttaaacat tttatcacct ctactcaaaa tttacactta
     1081 atttttaatt atataattct aatcctagac tagtttataa taattattgt ctctaattat
     1141 atcagatatt gagtaaattt aatactttgt aaaattattt atcctctctg accaaataat
     1201 catttccatc ttggtcttta aaagaaaaca tccttccata tggcatctcc attaattccc
     1261 caacctcaac ctcgttattt gccatttgtt cataagttcc attaatatct cttgtactta
     1321 aaattattga tggatgagat gcatttatct ctttgttttg cttcatcatt agatttttat
     1381 catacaacac aaatgttgta aattcatttt tacttggtgc tacttccacc catttcatat
     1441 ttggaccgat ttccttttca taagttacta caaagttcaa tttttctgtc caaaataact
     1501 ttgcatcctc ttgattattt acatagagag ttatttttcc tattttattt atcataacaa
     1561 tacccacctt tattattaaa taaataccta atatttttta tatttaataa tatacccata
     1621 tatattattt tctatagata tatatttcta atatttatcg ccaaattatg taattctgct
     1681 caatatataa tttataatat ccatactatt atagccttta tttatgtaat ttaatcacct
     1741 tacatatcat acttataatt atgataataa ctacataaaa aatcacttct aaatagagtt
     1801 atccatctaa aagtgatttc atatattcta tgcaactttg tgttttagcc actttgttcc
     1861 tcttaatctt aaacaatata aagtccctct aacaagccaa tcagtataca tacctatcca
     1921 aattcctaac actccaaatt ctaatacaac ccccaatacg taacctgtaa aaattctaaa
     1981 aatccacata cctattactg cagtcatcat tgtatatcta gtatcaccgg ctccctttag
     2041 ccctgctgat aatacaaagg atataggcca tattaccata gctaaacaat cacttcgaat
     2101 taattgtgtt gagatgtcaa ttacactagc attttgagta tacatacttg aaatccaggg
     2161 tgcaattggt ataaatatag cccctactat aaataaacaa attgttgcaa atttagttaa
     2221 atataataaa gtactcttag ctcctttaat atctccacgt cctatatact gtcccaccaa
     2281 ggttgtagca gacaaagcta aagcattccc aattacattt attatggatg caattgacat
     2341 tcctattgca ttggatgcaa ttgctgatgt tcccatagta actataaata cttgaacaat
     2401 caatttacct gcattaaaaa gtagttgttc aactccagct ggtattccta tattaaatat
     2461 agacttttga gtttcaacat caatcttaaa tggaaatatt ttacccatag atataaattc
     2521 gtttccttta aataaagctc ttataattac tatgcatcca attattctag ctatagcaat
     2581 agatatagct gcacctgtta tgccatagga tggtgtttgc aaatttattc ctagaggatt
     2641 taaatcaata ccatatataa gtatatatcc caatgttaca tttactatat tcataaacat
     2701 agtcacatac ataggtttct ttacatctcc acaccctctt aaaataccat ttgctatttg
     2761 ttgtgtcgca ataaatggat acgtaaataa tgtaaactct atgtataatt ttgcatttga
     2821 ttttaccaat tcttctgcgg aaccataaaa taaattaatt acaccagttc taaaaaccca
     2881 catcaatatt gttattgtaa aggataatat tactccagac actagagcct gcttaatagt
     2941 ttcattcacc ttttcactat ctttttttcc tatttgttga gctacaacaa cagtagctcc
     3001 aacagatagt gcagcaaata cagatataaa tatattgttt attgaatcaa ccattccaac
     3061 tgcagataca gcttcttccc caatatgacc tgccatcatt gtattaaata ctcctaaaag
     3121 catgacaaac gtctgctcta tcattatagg aattgctaac tttataacat ttttccttat
     3181 aaacatccct attcctcttt tccctcttta tataaattta atttttaatt agtttatcga
     3241 actgtaaatt gacaatgcaa agtttagaat tgtattgttc gcattataat atactgcttt
     3301 caaataatat aaatcttgtt taatatcatt tgaacacagt atatctttat aattatctta
     3361 tgtttattat tttttatgga taccatattt aaaatattta ttaatttaat ataaatattt
     3421 tccgttatta ttaaaagtag ctatatttcg ttaatattgt atcactatcg tgtagcaata
     3481 acaataagta cattttatat attttttata atattattta atcatccaaa cttttaccac
     3541 tttttcaata tataaaatat tacactttag atgtaacaaa atcgcatcca tttaaatatt
     3601 gtattttata gactaatttt aggatgtgat actttggtta atttaaattc aaataatcta
     3661 aatgtaaata tggtttatcc tatttcttcc acagcaccca tacgtttcag aagatatact
     3721 gtttcatatt tagaaaacat ggaaaatatt tatttaacta tagcaacata ctttgataca
     3781 cttggtttag gcacaatgaa tatcgacaaa gtttatggtc aatgggcttg gtttactgat
     3841 aatatatatg atttaaatct atttgttttt gtaggaaatt atccttatca aattgctaaa
     3901 aacagatatg atacatttgt agatattttg ccagtagcta ttacttctat tgtcaatgga
     3961 gatagagtat ttttaatttc aaatccttgt ttgttaaatt caaaaattac agttaggttt
     4021 atatctagtc atcctactct taatagaaca gttgattatg gcaatattag agattttatt
     4081 ttttaaaact gcaacatgat gtgctattaa aaaataccct aaatttaatg atattattaa
     4141 ataactagaa tatttttaat tggcacatca tgcctagcta ttataaattc ataatattat
     4201 tttacttaag ataagtactg tattttatct acttgtatat ttttatttac ctagtttctc
     4261 agttctatat cctatttctt tattagctag cttataacct tcttgtatca tttcttctct
     4321 cgttaatgca ctccctaata aattgtcaat actcagttgc tttctatcac taaaatactt
     4381 atccaataat gattcctcta tatcttcctg atatgccaaa tttccttcat gcattatata
     4441 ttttacatca aaaccttctt tatttaatac ttttcccacc aagatacttc tatgacatct
     4501 tataggttct tgcatagctc ctaataaaac tatcctataa cccatttgac aacccttttt
     4561 taatctttct attccgttta aaaaaatatc ttcttttgct actttctcaa aatctgcata
     4621 accttcttgc gtatatacat ctttattagt tcttttagct ccaaattctt ttcccatgta
     4681 tatatataaa aatccggaat ttctaagaga ttcgttaaat gcctctttat tgtattgaat
     4741 attatattta gaatatggag tttctctaat gtcaactaca caatttatgt catatttttt
     4801 tatcatatta ataagcttat catatggata atttgaatgt cctattgcga atatttccat
     4861 ataaacaccc tcctattctt tgaaattagt atctatatat gtagataccc gattt
//
LOCUS       DAGSHT010000234         4850 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.240, whole genome shotgun sequence.
ACCESSION   DAGSHT010000234 DAGSHT010000000
VERSION     DAGSHT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.240"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            455..1486
                     /locus_tag="MJB72_003312"
     CDS             455..1486
                     /locus_tag="MJB72_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBY8264375.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1619..2917
                     /locus_tag="MJB72_003313"
     CDS             1619..2917
                     /locus_tag="MJB72_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBY8264376.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3031..3276)
                     /locus_tag="MJB72_003314"
     CDS             complement(3031..3276)
                     /locus_tag="MJB72_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBY8264377.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3424..4290
                     /locus_tag="MJB72_003315"
     CDS             3424..4290
                     /locus_tag="MJB72_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBY8264378.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4294..4830)
                     /locus_tag="MJB72_003316"
     CDS             complement(4294..4830)
                     /locus_tag="MJB72_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBY8264379.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
BASE COUNT         1966 a          500 c          773 g         1611 t
ORIGIN      
        1 tagtattttt tcaccttttg attcatactt tgtagaatgg tgtattttct aaaactgatt
       61 ataagacaag ttcattcaaa ttagcatttt tatatcatat aaataattgt tccccctaaa
      121 taagatttag atgtatagta ttaaaatcaa aaagtataaa ataaaattga tgcttaatca
      181 tagataaata ttaataaaat atacaagggg attgtctaat aaaaagataa tctccttttt
      241 tacaaataat tctaaattgt gatataaatc taaaaatttt atcttaacat ttaaccattt
      301 aatgataata attgatataa taaatataaa ttaaatatat ggtgtactta ttttatgttt
      361 ttagcaagaa atatagcatt taaaagataa attgtgcaac ttaaatcaaa gtttaagatg
      421 aactattaac atagatatat taaaaacttt tgtaatggag aatatatgtg atatgaataa
      481 taaagttaag gttgcagtat tagatacagg aatagacaaa gaacatgatt acttaaaaga
      541 taatttagtt ggaggaattg cttttgaatg tatgcatgat tatatcttta tatcagataa
      601 atttgatgat gaagatggtc atggaactgc ttgtgcatca ataataaaga aagagtatga
      661 agatgtagag ttatttgtaa taaaaatttt gagaaaagat ggtattacaa atataaaagt
      721 tcttgaagaa gcattaagat accttttaga cactaacata agattaatta atttaagttt
      781 atcagtcata ggagtagaaa gtgttaaggg cttatttgaa gtttgttatg aattgtttag
      841 aaaaggaaaa ataatagtat gttcactcgc taatgatttt gacttaagtt atccagctat
      901 gtttaacaat gttataggtg taagaggatt tactttagat ggagatgatt cattttggta
      961 caacaaaaaa tatgatgttc aatgtgttat ggatagcaat tcatatataa gttgtgatat
     1021 aaataattcc tatagattac caccaaaatg taatagttat ttatcagcaa agcttactgg
     1081 taaaatagct aagatattat cagaaaaacc aggcataaca atatctgatt taaatgataa
     1141 gcttgaatca ttagcaacta gaaatcattg ggatagttgt gattttgatg agtgtagtag
     1201 aattgtaaac tttaaattgg atttgtatga caaagaaaat gctcttttta tgaaagttgc
     1261 tgacgttgtt agggattgct tagatattga agagaataat gaaaaactct ttaagggtag
     1321 cctttttaat aaggagatag gcttggttta tgataattgt tttaatctgt tgaaaaagct
     1381 agagcataga tttgacataa agtttaatta tatggacatc tccaaatatg acttagtttc
     1441 aatatataca ctgactgaat tagtggaaag atataaaaac ttataaataa agatttgtag
     1501 ctaaatttaa tgtagaaggt attaaattat atgtattctt tgtcagtaga ttaaaagtaa
     1561 aaaatatact ataattagat agcataagag aaaatgccaa tacaggggga gttagataat
     1621 gaaatatgaa gaagcattag agtacatatc acaaactaat aaattcggta taagattagg
     1681 attagaaaat atcggaaagt tattagaatt attaggaaat ccacaagaaa ctttgaatat
     1741 aatacatgta gctggaacta atggaaaagg ttctgtgtgt tcttttgttt ctaatatact
     1801 aagagagtgt ggatataaag ttggtttata tacatctcca tatttagaaa cttttactga
     1861 gcgtataaga gtaaatggac aaaatatacc acaggatgat gttgctagaa taattgagtt
     1921 aataaaagaa aaaatagaga ttatggtaaa agaaggctat gcatatccga cagaatttga
     1981 agtggttact gcaatggcat tttattacta ttcagaacaa aaggtagact ttgtggcatt
     2041 agaagttggt ttaggtggca gatatgatgc tacaaatata ataacaaaat cacttgtaag
     2101 tgtgatagta tctataagtt tggaccatac aggtatactt ggagatacaa tagaaaaaat
     2161 agcgtacgaa aaggctggaa taataaagga aaatggagta gtactagtat atgaccagac
     2221 tgatgaagca aaggatgtta taaagtcagt ttgtaaagag aaaaaagcta aatatataga
     2281 agttgatttt gatgatataa acataaaaaa atcagatata aattctcaaa tatacgattg
     2341 tactgtaatg aaagaaactt atagagacct tgaaataaaa ttgataggcg aacatcaaat
     2401 aaacaattct atcttggcaa ttagtgtaat aaaatatcta aaagacataa ataaattagc
     2461 aaatataaat gaagaatcta taagaaaagg acttataaat actaaatggc caggaagaat
     2521 agaaaagata aaagaaaatc caatttttat aatagatggt gcacataatg aagatggagc
     2581 aaaatctttg gcaaaagcac ttgataagaa ttttaaaggt agaaaattaa ccttattaat
     2641 aggtatgtta gaagataaag atattgatag tgttttagag atattgttgc cacattttaa
     2701 taaggtaata acaacaacac caaataaccc aagggccata aattcagata tattaagaga
     2761 aaaagtatta aaatatgtag atgatgtaac atcaaagcat gagatagagg atgcagtaaa
     2821 ttatacatta gaaacatcaa gtgaggatga tattataata agtgcaggtt cattatatat
     2881 gattggtaca gtgagaactt tagtaaagaa attataattt tataaaataa atatacaaat
     2941 aaaaatataa aaaagagttg tctaaaaaat ggacaactct ttttatataa aataagtttt
     3001 taataatttt actgatgttt atataagtaa ttatatagat tgacgtatag ctttagataa
     3061 ttggtctggg caagaagtac cttttccacc acagtcaata ccatttaatt tatcagcaac
     3121 ttcaagagca ttttggccaa ttattaagct tctaagtcct gataaattac cattacatcc
     3181 tcctataaac tcagcatcaa caatcacatt attttcatca acttcaaaaa tcatttctct
     3241 acaacataca ccacttggat taaatgttac tttcattaaa aagacctcca ttaaatatta
     3301 tttctttttt taacattaat aataatacca aatatatgcg taataatgca actataaagc
     3361 ataatacaag ctaatgaaat aatatattat tgtagtatat gtattgtaga gaggaggggt
     3421 tatatgaaaa tctatatttc taactactta tcaaagagta ttagcatagt tgattattct
     3481 acacttgaac tagaaaaaga aatagtatta gaagataata tatatccaca ccatttttgc
     3541 atagaaaaag aaaaaaattt aatatacata cctagttcaa gtaatgggat actctatgtc
     3601 ttagatttaa gtaatgataa aataattgat actgtctcta taggaggaag tcttagtcaa
     3661 gttgtactta gcgataatga attgtttgta gcaaatgaag attctaatag tatatatgta
     3721 ctagataaaa acacattaaa tccaatagga attattggag ttgataatat gccacatgga
     3781 tttgactttg atagagaaag caaaaagtta tatgtaccgt gtataaactc gatagtatgt
     3841 atagatacaa ttaataaaaa tatacaaaag aaaataaata tggactttag agcttggcat
     3901 atagaacttg ataaacaaaa gaaagaaatg tatatatcaa cacttgatgg aaagcttata
     3961 ataatagatg aagtaagcat ggatataaaa aaggtattat atgaattttt attaccagta
     4021 gaaataagat ttaattatag tggcaaaaaa gtttatgttg ctgatttggg ttataataat
     4081 gtaagaattt tagattatac aacgagtaga tacattggaa atattgaaat aaatgggatt
     4141 ccccaaggat tagaaatatc taaagatgaa aagttactat ttgtatcaga tacacaagaa
     4201 aattcagtaa aagtttatga aactgcaaat aacaaattaa taaaagaaat caaggtagga
     4261 aaagagccta caactataat ttgtttgtag gctttaatgt atgtctctta taagtaaatc
     4321 gataacttcg gcatacatat aagatgcatt atttttccag ttattgcaaa taagttttgc
     4381 ttgtttctct gatgaaacat ttagatttaa atctattaag ttagactgat tttctatgac
     4441 tctaaggttg acaataaatt cattatcact ttgttttaca aaactagact ttacttgagt
     4501 atctgctagt agattttctt tgttagaatc tatatacttg tctatttttt cagtaacact
     4561 agtaggtatt cgattaaaga agtattctag actttctacg cctctttgag taagagcata
     4621 atattctctg tcagagtctt tatatgtagt caaaaactta gattccataa gctgagataa
     4681 aagctgttgt aatgaaaaat aattcatcat ctcagtctct aatactactt gagttatttg
     4741 agaattagtt aaatccattt taattttttc taatatatat aaaattaaaa gtttgtgata
     4801 ggctaattct tcagacgagt tttcaaacat aaatatcatc ctccgtcaaa
//
LOCUS       DAGSHT010000235         4848 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.241, whole genome shotgun sequence.
ACCESSION   DAGSHT010000235 DAGSHT010000000
VERSION     DAGSHT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.241"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..177
                     /locus_tag="MJB72_003317"
     CDS             <1..177
                     /locus_tag="MJB72_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264380.1"
                     /translation="IKSIVSLIKQLGIRVVAEGIETRSQIEMLKKANCDIVQGYYFSK
                     PLPIKEFEKLVYKI"
     gene            210..3647
                     /locus_tag="MJB72_003318"
     CDS             210..3647
                     /locus_tag="MJB72_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBY8264381.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKNASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            3665..4411
                     /locus_tag="MJB72_003319"
     CDS             3665..4411
                     /locus_tag="MJB72_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBY8264382.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(4360..4668)
                     /locus_tag="MJB72_003320"
     CDS             complement(4360..4668)
                     /locus_tag="MJB72_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY8264383.1"
                     /translation="MNFFKENSTFYFATVKDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2042 a          493 c          725 g         1588 t
ORIGIN      
        1 ataaaaagca tagtttctct cattaagcaa ttaggaataa gggttgttgc tgaaggaatt
       61 gaaacaagaa gtcaaataga aatgttaaaa aaagcaaatt gtgatattgt tcaaggatat
      121 tatttttcaa aaccattacc aattaaagaa tttgaaaaat tagtctataa aatatagtca
      181 gcgagtctta aatgggaggt ggtgattaag tgataagagc aaaatcgtta aaaaatgtac
      241 tgagtattgt tttaatattt ttatttacaa taatttcttt ttctgatttt gcatttaaaa
      301 ctaatgtcca ggaagaagaa aatagtaaaa ttaatttaag ggaaatatcc ctacaaagtt
      361 ccaatgctat aaaaataaaa ttagatagtt ctctatcctt tattaaaagt gtggcaaata
      421 tggtttctta ttttaatact atagataact caaaaatagt tccattatta gctaatatat
      481 caaaagaatc tgactttcaa agaatctcta ttgtatctcc tgatggaata agtcatacca
      541 gtgatggcaa aattatcaat gtgtcaaagt atcagtattt taagaaagct attaaaggag
      601 aaagtaatat atctgaagtt gtgaaatctc caattgatgg aaaagatact tttgttatag
      661 ctactcctgt atttaataag aataacaaaa taattggtgt tttatacggt tcttactaca
      721 atgataaatt agctgaattt atagatataa caagttttaa tagtgaaggg tacatagata
      781 tatttgaatc tagtggtcaa tttgttttaa aatcttcaca tacaaacaaa ctgattaagt
      841 caagtagtaa catatatgat atatttaaaa atgcttcttt tgatgatggt tatagctata
      901 aaaaaatata tgaagataca aaaaataaca agagtggctt tacctcatat gaatacaaag
      961 gagaaaatcg ttatgctact tatacaccca ttggtgtaaa tgactggtat gtaatttctg
     1021 tgataccaca atatactgta agaagaaaca ttgataaaat aaatcaatta gcattttctc
     1081 ttattttcaa ggtattctta ttgtttatta tgcttataat atatattatt tataaaaaca
     1141 ataaatctca gaaagatata ctaaaagcta acaaagaaat ctcatctatt acaaatagca
     1201 ttcctggagg agtacaaaag tgtaccctgg gtgaaacatg caatattaat tttttaagtg
     1261 atggatttat agagttgttt ggttatacaa gagaagaaat aaaaacattg tttaataatg
     1321 atttttatag tttaatacat aaagatgatg ttgaaagaat aaaaaaagaa atttttaaac
     1381 aactttacaa aaataaaact attgaattag aatataggat tataacaaaa aatggtaata
     1441 cagtatgggt tttagataaa ggggagctta taacagaacc tgatggaaaa gaatattttt
     1501 gttgtgtaac tgtagatatt actagaacaa aacaattaga acaagagatg gaacaaaaaa
     1561 atctagaact tgaaatgata tcagaaaatg ttttaggtgg tataattata acagaattta
     1621 atgatgattt tactataaaa tacgctaact ctggttatct ttcaatgatt ggatatacac
     1681 gtgaacaatt agaaaatgaa cttaaataca aagcttgtaa tcttgttttt tatgaggata
     1741 aaaagaaggc tcttgaaagt atacaacatc aattaaacac aaaaggaata gctgtatatc
     1801 aacatagaat aagaagaaga aatggtaata ttatttgggt ttctataaaa ggaaggctta
     1861 ttgttgatga tgatggtatt gaaaaaggta tttggattct aaatgatata acagatttaa
     1921 aaaatgcaga acaaaaactt aaaataaatg aagaaagatt tagaattgca ttaaagcaaa
     1981 catcaaacac tatatttgag tatgatataa aaacaaagtc acttttaaat actagtaaag
     2041 agcttgataa atataatatt tataaaatta ctaaaaacgt tccatattct ctagtagaat
     2101 caggagttat tcataaagat tttagtgatg attttataaa catgtatgaa aatgtaataa
     2161 atggagcaaa aaaagcaagt tgtgtagtta agatgaaact tccagatgaa aattatatat
     2221 ggaataaagt taccttgact actatttttg atgaagatgg taatcctgta agagcaattg
     2281 gtatacagga agatataagc aaacaaaagg aagcagagat aagatacaat caagaaggta
     2341 agtatcattc agctatattg tcggaagcaa tgataaccta tgaaataaat gtatcaaagg
     2401 atacttttat caaaggaaat gaaagttggc aaaaaccatt tggaattaag cctgacaata
     2461 actattctca aatggtaaaa actatagcaa atagtattgt acataaagac aataaagaaa
     2521 catttttaaa tacattttta tgtgaaaact tattggatgc atataaaaat aatgtgactg
     2581 aaataaaatt agaatatcga agaatgaatc aagaaaataa aatgatttgg gtcatttgta
     2641 caatgcactt aataaaagat tctattacga acgatataaa agcacttgct tatgttaaag
     2701 atattgacaa gcaaaaaaat gaagaaatta gattaaaatt aaaagctgaa agagatactc
     2761 ttacaggatt atataataaa ggtactacag aaaaattgat taatgatttc ttgaactctg
     2821 aggaagcaag cttaggaatg catgcatttt taattgttga tatagataat tttaaatctg
     2881 tgaatgataa tttaggacat gtatttggag ataaagtatt atttgaagtt tctaaaaacc
     2941 taaaacctat atttagaaaa gatgatatag taggtagaat tggtggagat gaatttatag
     3001 tattcttaaa aaatattcag tcttttgaga atatcaaaaa gaaagcagaa aaaatatgcg
     3061 atatatttag aaattcttat acagaaaata aaaagacata taaaatatct ggaacagttg
     3121 gaatttcatt tgtaccagaa catggaactg tatttgaaga actttataaa aaagcagaca
     3181 tagcacttta ttatgcaaag agcaaaggaa aagataagta tgcactttat aatgattgta
     3241 tctaccaaaa tgaacttgaa aataatataa gtgataataa tataaaatta gcagataaat
     3301 gtgagaaatc caaaccagta cttaaagaaa ttcttgattc tattgataat tatatctata
     3361 tagtgaatcc aaatacttat gaacttcttt ttgtcaataa taaggtgtct cgtttggatt
     3421 tagagataaa attaggagaa acctgttata aatcactatt aaaatctgat agaccatgta
     3481 ataattgccc tcttaacgga ttagaagaaa ataagcattc aaaattcaaa atagagacaa
     3541 atacatttgg taaaaatatc agtatagcaa gttggattaa ttgcatagat ggagaataca
     3601 actgtcttat agagtgtata gattcttctt tatacaaaaa taaataaaaa aggactatga
     3661 agatatgtta aatagtataa ctttgatact tggtataata tttacaattt actattttta
     3721 tctcaaaata gtttttggag gaatttcatt tagtgagatt ttcttagttg taggaattgt
     3781 actaattatc tatcaaatat ttaagaaaaa aattaaagaa aagaaggttt tttatagggt
     3841 gttaaaaata ataatcagca tttttttaat agtatttgta gttactgaga gtctaattat
     3901 tttttatcct aaaaatagtt tagaatcaaa aagtgattat ctcttaattt taggagcttc
     3961 tgtaaaaaag actacaccaa gtacaaccct gaagggtagg ttagacactg cattgaaata
     4021 cctaaaaatt aatgataatt gttatgtggt agtatctggt ggtaaaggaa gtggagaaaa
     4081 aataactgag gcaaaagcta tgaaagatta tcttataaaa aatggtatag ataaaaatag
     4141 gataatagaa gaggataaat ccacgaatac ttatgagaat tttaaatatt caaaattgaa
     4201 gatagaagaa catagtcaaa gaaaactaag cgatttaaaa gtaaaaatag taacaacaga
     4261 ttttcatgtt ctcagaagta aaatattagc atatagaaat ggatataaaa atacgtcttt
     4321 ttatgctagt aaatcaaaac tttcatttgt accaacttac tatacaagag aattttttgc
     4381 attatggaaa acaatcattt ttgatagata ataaatgaaa agactagata taaactcata
     4441 tctagtcttt tctattccct taattctaat ccattttcca actttacttg ttgtagaaat
     4501 ttcaacattg ggatttacaa gtaattgctt ataagaagtt ttatatttta cgattccaaa
     4561 atataatttc ccttcataat ccattataaa tccgaatggt cttactcttg gtttattatc
     4621 tttaactgta gcaaaataga atgttgagtt ttctttaaaa aaattcatta ttttttcatg
     4681 attaaatcct ctttgtatct tatactaata taatgaatat aaataaaata ttcataatac
     4741 taatataaat cgatataaaa acaattacaa tattatattg attattcaaa attatatata
     4801 aaattcagaa aaaattttag tttacataat aaaaataaag tcaactaa
//
LOCUS       DAGSHT010000236         4816 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.242, whole genome shotgun sequence.
ACCESSION   DAGSHT010000236 DAGSHT010000000
VERSION     DAGSHT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.242"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..604
                     /locus_tag="MJB72_003321"
     CDS             <1..604
                     /locus_tag="MJB72_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264384.1"
                     /translation="SQSSIKTFDQSVRKLDKQFNILNIKFVKLFDKYDNQDNINKEIL
                     FDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSAQDLLTKLMTHENKISQNQKI
                     LLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSLDIQDKLDVMFNNSFMNDEVP
                     LDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDELNE"
     gene            597..1229
                     /locus_tag="MJB72_003322"
     CDS             597..1229
                     /locus_tag="MJB72_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY8264385.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            1558..2715
                     /locus_tag="MJB72_003323"
     CDS             1558..2715
                     /locus_tag="MJB72_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8264386.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(2862..3497)
                     /gene="lexA"
                     /locus_tag="MJB72_003324"
     CDS             complement(2862..3497)
                     /gene="lexA"
                     /locus_tag="MJB72_003324"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBY8264387.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(3601..4728)
                     /locus_tag="MJB72_003325"
     CDS             complement(3601..4728)
                     /locus_tag="MJB72_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY8264388.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         1774 a          615 c          711 g         1716 t
ORIGIN      
        1 ttctcaaagt tctattaaga catttgacca gagcgtaaga aaattagata aacagtttaa
       61 tattttaaat ataaaatttg taaagctttt tgataaatat gataatcaag ataatataaa
      121 taaagagatt ttatttgata taaaagaatc ttcaaaaaat atatacaatg ccactgaaag
      181 ccaattcaaa attagagatt atataaaaaa tataaatgca ggatttgctc tatatgaaag
      241 aagtgctcaa gatttattaa ctaaattaat gacccatgaa aataaaatat ctcaaaatca
      301 aaaaatcctt ttagatgaaa agtttacctt agatgactca ataaaaaatc tctcaagtat
      361 tattgaaaac ttttctaatg acctacaagt taaactggat atgatgtttg aaaattcttt
      421 agatattcaa gataagttag atgttatgtt taataactct tttatgaatg atgaagttcc
      481 tcttgatagt gaagaattat ttaaggatga tgttaataat gtatttaatc cttttagtga
      541 agaaatttat gagatagagg ataaagaaat aaaggtcata ggtgaggatg agttaaatga
      601 ataataaata tagaagaact gttaacagag aatttgaaaa gagtagcttt tggccaacat
      661 ttacagattt attatctact gtattgatgg ttgtgattct tatcttattt agctcagaaa
      721 gtatttcagg ttctgttgaa caagatttag ctaagaatgt caatgcttct gttgaagaaa
      781 cttttaaaaa aagtggaata cctgtgaagg tagataaatc taatgggcaa gtaacttttg
      841 gtgaaaaaac catgtttgat gtagatagtg atgttttaaa accagaggct aaagaaatgc
      901 taaaaatgtt tgttccaaag tacatagaga ctatttataa agattatggt gactatatat
      961 ccaaaatagt tatagaaggt catacagatg atgtgggaag ttatatatac aacttagatt
     1021 tatcgcaaag aagagcatat agcgttgcta aatttatagt gggagatgaa ataggtgact
     1081 ataaatacaa agataaagtg actaagcata ttattgcaat aggaaggtca aaagctgaac
     1141 tcatcaaaaa tggtgacaat agcgtgaata gagatgcatc tagaagagta gaactaaaat
     1201 acgaaataaa tattaatcaa aataaataat taaattttta taaaaaaaaa gaaggcagtt
     1261 ggtgtgagct ttaagccttc tttaaggaaa aagtttattt atttttgttg atacttaaca
     1321 tattatagtt tttagaagca gtttgcaata tattaaacgt aaatggtcgt ttaaaatgca
     1381 taactggata ctactgatgt aaaatatttc attttatata atataaaaat tatacagtgt
     1441 caattttcta tattattgta ctataatcta ttatttaaaa tattatgtaa aaaaatatca
     1501 taaaaacgca tatcatgtta taattactag agtattatga atatggaggc gtagtttgtg
     1561 atagaaataa gtataaaaaa taaaaaaaat tctaaaatta aaactttgat acttttattg
     1621 atagtatcta tatcgattta tttaactggt tgtactacat cagataatgg aaataatcaa
     1681 tcaaataata atgattcttt gtctagtagt atatcaagtg ataagaatgt aaaaattcat
     1741 tttattgaca ctggaaatag tgatgcaata ttgattcaag atggtaaaac ttttacattg
     1801 atagatgggg gagataatga tgatgaggga ttaatggtag attacctaaa caatcaaggt
     1861 gtaaaagata ttaaatatct tatagctacc cattctcatg cagaccattt aggtggactt
     1921 gattctgttg tcaaaaactt taatatagaa aatgtttttg tatcgaatgg ttcagcagaa
     1981 acaaagagtt atagggactt tataaatgct ttagcaaata agggtttatc cccaagtgta
     2041 ccgcttgaaa ataataagtt ctatttagaa gattcttatt ttgaagttct aaatacaaat
     2101 gggggagata ctactaatga acaatctcta gtccttgtat atactaatgg gaatgataaa
     2161 gttttattta caggtgatgc tgaagaaggt accgaaaaag aaatactacc taagcttgag
     2221 aaagtagacc ttttaaaagt agctcatcat ggttctcgta gtagttcatc tcaagagttt
     2281 ttagataaag taaatcctga atatgcggtt cttttagtag gcaagggtaa ttcatatggt
     2341 catccacatc aggagacaat gaataaatta aaaaaaatgg gagtaaaagt gcatagaagt
     2401 gatgagtgtt cagatataat atttgaatca acaggtgatg gagtatttac aagttgtaaa
     2461 gatggaagtt ataataaagg cgttagagaa gatggaaact ctggaaaatc aaacagtaat
     2521 acaagtaaaa atgattcttt taaaaataaa caaaatacat ccaattcaga agaaatagtg
     2581 tattttactc caaaaggtaa gagctatcac tctacaaaaa attgtagtgg gctttcaaga
     2641 agtaaaaaaa tattatcagg cacaattgca gaaagtaaga aaaatgaccc atgtgatata
     2701 tgttatggaa aataacaaaa aaacaaccaa tttatctata aagcttctga actttaaatc
     2761 attcaaaaaa taaaataaat gcataaaaaa ataagctata atcaatagat aacacatttt
     2821 atctatacga atagcttatt tttttaagta ttttttaata gctatttaat aactctaaat
     2881 acaccagtta caatacctac aatttcaact tctgagtcat tgagcataat tggttccata
     2941 aaatcatttt ctggctgaag tcttattaaa tttccttctt taaaaaacct cttaactgta
     3001 gcagattcac catttataag tgcaactact atctgagaat ttaaggctgt attttttttg
     3061 tctactacaa cgtaatcttc atctaaaatt cctgcattaa tcatactttc gccttttact
     3121 cttaatacaa aattgttact gccctttact aaactggcag ggaatggaat gtattcctct
     3181 atgttttgtt gagctaaaat gggttctcca gctgttattt gacctactaa aggaagctct
     3241 attatttctt gattagctcc agaaacatca tttactttac tacgttctaa aacttcaata
     3301 gctcttggtt tagtagggtc ctttcttatg tatcccagtt tttcaagttt atttagatga
     3361 gagtgcacag ttgaagtaga tcttaaccct acagcagtac atatttctct tacagcaggt
     3421 ggataacctt ttaatatgat ttgagacttt ataaattcca atatcaagac ttgcttttca
     3481 gttagatcta aatacataac atcactcatt tccaaacata ttttgttttt atttccatac
     3541 actttatatt tttaattata acataatcca tttatatctg caaacatttg ttcctgattt
     3601 ttaatcgtcc tcaatccatt caaaattctt gactatatca cgttttacat ttttcttatg
     3661 agctgcataa agctgagtag ttgtgacatt atcatggtca agtaagtttt gaacatcata
     3721 tatagaaaat cctgtttgga ttagagaagt tgcagccgtt gctcttaatt tatggggact
     3781 atatccattg tctctagtag tatcaagtgg aatagatgta tattttttta ccagtgttct
     3841 tatagccttt gctgtaattc tcttattttg aagagacaaa aataaagcat ctttttcttc
     3901 atcatttaat agttcatctc ttgttctttc gttttgcaaa taatctttta ttacatgttc
     3961 acaagtatga tttataggca ttaaaacttc tttcccacgt tttctatata ttttaaattc
     4021 acctcttgaa aaattaaaag aggagatatt gagttctcta agctcattta atctaagccc
     4081 gtatgtaaca aataatgcta gtatagcttt gtcacgtaat ttagtttttc tccaatatac
     4141 cttttctttc tctgtaagcc cttcacctgt ttctactgat tcaagcatct tagcaacttc
     4201 atctatctca aggcgcttaa tagcatctgg ctgaggttta ggaagtttta ttggattaaa
     4261 tccatcagtt atattattat ctatctgaga gtttctgtat aagaatttaa atagagtaga
     4321 gatggatgat ttttttcttg ctaaagctct attgttattt tcaaaaatca atgtgttttt
     4381 ttctgtattt ttgtaatatc tactgcaata atcacctaag aataaattta tatctcttga
     4441 ttttattgta ttaaaatcat cttctgtaat atcttttaca caatctgcat ttgttaattc
     4501 tttggtttca atcaaatatg aacaaaaaaa attaatatct tctaaataag caagtcgtgt
     4561 agaaactgca acagaacctt ttaaatatat aaaataatcc ttcataaata caggaagttt
     4621 attttccaaa agtagacatt tttctatgag ttcattttct ttgataacaa tattatcagg
     4681 tttatctgat ttattatgta cttttataat tttcttatca ttattcaaaa ttaaacctcc
     4741 taaaactaaa atataaatct tttatattat tttttgatat ttataccaag tgagtataaa
     4801 tatttaattt aatcat
//
LOCUS       DAGSHT010000237         4806 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.243, whole genome shotgun sequence.
ACCESSION   DAGSHT010000237 DAGSHT010000000
VERSION     DAGSHT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.243"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     misc_binding    300..537
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            601..2520
                     /gene="thrS"
                     /locus_tag="MJB72_003326"
     CDS             601..2520
                     /gene="thrS"
                     /locus_tag="MJB72_003326"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBY8264389.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3288..4379
                     /locus_tag="MJB72_003327"
     CDS             3288..4379
                     /locus_tag="MJB72_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY8264390.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1899 a          475 c          859 g         1573 t
ORIGIN      
        1 agtattgatt gtatttaagt atacaaaacc taaaaagagt acaaaaatta gaagttagtt
       61 ttaataaaaa ttagttttag tatttatcta gtcatatcta tcatgcatgt ggatacgttt
      121 taaaaatttt aaatttatga aaatattata atatcactat aaatcaagag attaatttaa
      181 tataaaaata taagaatatt ggtttacagc tgattgagat gaaaaataat gggtaattaa
      241 tatattacat taatgttgat tgatgtataa ctatctgtta taatgaaaat atattaaaaa
      301 tcatagtgtt gaagaggaag agtagagtag aaaccattct cagagaattt gggtctatgg
      361 ctgagagccc atgtaatagg tctatttgaa aactaccttg gagctgagct tattagcagt
      421 atttgttttg tactatacgt gaaagtgctc gggtgacgac cttatagtca tcaagagggt
      481 tgtatatact tgtatacgcc aacttgggtg gaaccgcgaa ttaatatcgt cccaaacata
      541 tttggggcga ttttttatta caaaaaatct aaaaattatt tcaataagga ggaaaaaaca
      601 atgattaaag ttgctttaaa agatggctca ataaaagaat ttgaaaatgc tatttctgtt
      661 atggatgtag ctaaatccat tagtgaagga ttagctagaa atgtggtagc agcgtctgtg
      721 aacggagagg ttgtaggtct ggaccacatt atagatactg attgtgactt gaatttattt
      781 aagtttgaag ataaagaagg taaggaagtt tttagacata catcagcgca tatattagct
      841 caagctataa aaagacttta cccagaagca aagcttgcta taggaccaag tatagaaaat
      901 ggattctact atgatataga tttagaccat agattagttc cagaggacct agagaaaata
      961 gaagctgaaa tgaaaaaaat agctaaagaa gatttaaaaa tagaaagatt tgaacttcct
     1021 agaaatgaag ctttagagct tatgaaagaa caaggagaag actataaggt agaacttata
     1081 tctgatttac ctgaaagtga aataatatct ttctacaagc aaggtgattt tactgattta
     1141 tgtagaggac cacatttacc atctactaaa aaggtaaaag ctgttaaatt acaaagtgta
     1201 gcaggtgcat attggcgtgg tgacgaaaat aataagatgc ttcaaagaat atatggaact
     1261 tcttttgaga aaaacaaaga cttagaagag tacttacatt tactagaaga agctaaaaag
     1321 agagaccaca gaaaattagg taaagaatta ggattattta tgatacctga agaaggtcca
     1381 ggattcccta tgtttttacc aaaaggtatg gaacttaaaa atgaattatt aaaattctgg
     1441 agagaaatac acagaaaagc tggatatatt gagatagaat ctccaatcat attaaataga
     1501 aaattatggg agacttcagg acactggtat cattacaaag aaaatatgta tacagttaaa
     1561 atagatgatg aggattatgc tataaaacct atgaactgtc ctggtggatt aatttactat
     1621 aactctcaac tacattcata tagagatttc ccaatgagag ttgcagagct tggtagagtt
     1681 catagacatg agttatctgg tgcattacaa ggattaatga gagttagagc atttactcaa
     1741 gacgattcac atatattcat gttaccagag caaattaagg acgaaataaa aggagtagct
     1801 aacttaatag atggtatata caaaactttt ggatttgaat ataatttaga attatctaca
     1861 agaccagaga actctatggg aagcgacgaa gagtgggaag cagcagaaaa tggattaaga
     1921 gaagctttag aagagttagg tctaccatat actattaatg agggtgatgg agcattctat
     1981 ggtcctaaga tagacttcca tctaaaagat tgtttaggaa gaacttggca atgtggtact
     2041 atccaattag atatgcaact tccaagacaa tttgataata cttatattgg tcaagatggt
     2101 gaaaagcata gaccagttat gattcatagg gttgcttttg gtagtataga aagatttata
     2161 ggtatattaa tagagcatta tgctggtaaa ttcccagttt ggttatctcc aactcaagtt
     2221 aagatacttc ctatatctga taaatttatg gattatgcaa atgaagttaa gaaagagtta
     2281 tttgataagg gtataagagt tgaattagat gatagagctg aaaaaattgg atttaagata
     2341 agagaagctc agcttgaaaa agtgccatac atgttaatag taggtgaaaa agaagtagct
     2401 gataataatg tttctgttag atcaagagat aaaggtgaaa taggtagtat taaattagat
     2461 gaatttatag ctagtatttc aaaagaaatt gaatcaagag aaagtattat tcaagactaa
     2521 tatatattat tttagtagct aagatattgt tatataagat aagtttaatc aatatgttga
     2581 tatgttgatg catagtgatt aaatttatga aaagatgagt ataattagag gtacagttaa
     2641 tcatattttt atgattaatg tacctctagt tttttgtatg gtagatttat taataaatat
     2701 atcatagaag tgttttgaac atggaaatat ttatgtgcat tctttaaata aaagtttatt
     2761 ttttgagtga taattaagtg cttgttggta aggttatggt tgtaaaaatc ataactttat
     2821 tttcatattt gttccatttt cataaaaatt attatccctt taagaaaatt tcttaatcct
     2881 cttgaaatat ttccttttta gtctaaagtt aaatttcttt cttcaaaatg aatatttaaa
     2941 tgaactaaaa tttaagtaca ttttctaatc ttatgagtct atttttcata gataattgcc
     3001 ctataataga agaatctatg ttggagctat gtggtgacaa taacagtatt gtcagtgatt
     3061 aattaatgct ttataaatga ttaattagtg ttttatgtta tttttggact taccctctat
     3121 acatagatta atttatttaa ctttagatag agagagaatg tcaagtacat acacagacat
     3181 tagagtttaa gaaagtgttt atggagcaag aacttttaaa agaggtagtg acttagttta
     3241 atttcaagaa atgataaata caaaagagga aaaagagagg tgttgaaatg atagaaaaag
     3301 tagaagaaaa atctaagatt attaaaacat caaaagaata taagcgaact ataattgaag
     3361 aaaaagataa agaatggacc aaaaaattaa atgatatact tagtagaagt agtttttcta
     3421 atgatgaatt agataaaatc aaggcgttaa ttccaaaaga gattttgact aatgaaaatg
     3481 ttggagaagt tgtgactgaa gatggatatg ctaagcccga gtatgatgag gtatttaaat
     3541 cattgttcac tttaaataat gaatatgatt tacttgtaaa ttttataaat gatatcctaa
     3601 aagatgctcc atatgctaac aggaatatca aaccgttcac tcaaataaag agaataatta
     3661 gagttgaaac tgacccaaca attaattaca ttggagagaa aagacctaga cttgatattt
     3721 tggctgaaga tgaagaaaat aatcatataa atattgagat gcaaagagct tttgagaacg
     3781 attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag ttagaagaag
     3841 gtaaagagta taaagaaatt ggaaaaacta ttggaataca tatactaaat catgtaaaat
     3901 ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg catccagata
     3961 tttattctag taaaactgca ttatatttta tagagcttcc aaaaattcat aaatctgact
     4021 atatagtaaa tagaattatg ctatggggaa aacttattag taatccttct agcttagatg
     4081 taagagttat agctaagaat gattctatta ttaaaaaagc acttgataga ttaaaagaat
     4141 taggtagcaa tgaagaatat ttattaaatc tgaaaagagg tgcttatatc atgaataaaa
     4201 gtagaaattt taaagaagag attgaaagag agacagcaat tagagttgca aaagaaaaag
     4261 attataaaat aattataatg ttgaaaaaaa atggttttaa attagatgac atattaaata
     4321 aatttaatga ttttgactta agtgaagatg agataaaaga agtatatgaa aataattgat
     4381 gataaaaagt gaacttatat aagtttgctg aatcggagtc tggaaatatt tatgtgtatt
     4441 ttttaatgaa taaaaattta ttttaaaaac aataatttag aggtacagtt aattatattt
     4501 ttatgatact gtacttctag ttttttctat tgtagattta ttatttacta ataaatctac
     4561 aatagaagtg cttttgttgt aagaatcatt ttatctaatg attgtttttt ataatagtta
     4621 aatttccatt tgtagtgttt aaatataaat ctataacagc atttctataa atgttataaa
     4681 tcactataat gattcaactc aaagttactg cttaaagaag cagagatttt atcaaggtat
     4741 atcatggaaa aaaatatttt attgtaatat ttataagatt ttattatgga aataatgtaa
     4801 aaactt
//
LOCUS       DAGSHT010000238         4721 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.244, whole genome shotgun sequence.
ACCESSION   DAGSHT010000238 DAGSHT010000000
VERSION     DAGSHT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.244"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            47..964
                     /locus_tag="MJB72_003328"
     CDS             47..964
                     /locus_tag="MJB72_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBY8264391.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            1106..2290
                     /locus_tag="MJB72_003329"
     CDS             1106..2290
                     /locus_tag="MJB72_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY8264392.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            2397..2585
                     /locus_tag="MJB72_003330"
     CDS             2397..2585
                     /locus_tag="MJB72_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBY8264393.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            2734..3384
                     /locus_tag="MJB72_003331"
     CDS             2734..3384
                     /locus_tag="MJB72_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY8264394.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            3487..4425
                     /locus_tag="MJB72_003332"
     CDS             3487..4425
                     /locus_tag="MJB72_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY8264395.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            4451..>4721
                     /locus_tag="MJB72_003333"
     CDS             4451..>4721
                     /locus_tag="MJB72_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8264396.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDI"
BASE COUNT         1961 a          447 c          860 g         1453 t
ORIGIN      
        1 tgaattaatg aaagacatga attaattaga aaggaaggca aattaaatga acaaaataga
       61 tttaaatctt ttagaaaaag ttgctgaact tcgtggaact ccattaggag catacaatat
      121 acgtaaaaat gggcaaggag tagagagaca ttcaactgaa aatgtaacta taattcctaa
      181 aactgataaa cctggaatag atattatagt aaagccaaat acaaaaggag aaaatgtaca
      241 tataccagta atagtaacag aggctggtgt acaggatatg gtatataatg attttgaaat
      301 tggagaaaat gcagatgttg ttataattgc tgggtgtggt atacataact gtggttgtga
      361 tgatagtgaa cataatggta tacatagatt tttcttacgt aaaggagctc gcctaaaata
      421 tgtagaaaaa cattatggtg aaggtgatgg agaaggaaag agaattttaa atccagttac
      481 tattgtcaat atggatgagg attcatattg tgaaatggaa acaattcaaa ttaagggtgt
      541 agattcaact aaaagagaaa tggatgcaaa cctaggtaaa ggagctaaaa tagttgtatt
      601 tgagaaatta ttaactcatg gtaatcaagt tgcagaatct aatatgaagg ttatcttaga
      661 gggtgaaaac tcttctgctc aagtagtttc tcgtacagtt gggcaagata attctaagca
      721 aaagtttaat ccttgtgtta ttggggattc cctttgttct gcacatgttc aatgtgattc
      781 aataattatg ggaaattctc aaattagtgc tatacctgaa attgctgcaa atcatccaga
      841 tgctctttta gtacatgaag cagctattgg aaagattgca ggagaccaaa tactaaagat
      901 gatgacatta ggtcttactg aagaagaagc cgagcaacaa atacttaatt gcttcttaaa
      961 gtaaattaaa gattttattt aatttaatat aattaatata gtataatata gtgaataagt
     1021 ctaaaatatt tttattttta ggcttatttt ctttatgtaa gaaaagctat tagagaaatt
     1081 atattgtaat taggatgtgg aataaatgaa aaatttactt ataagaaatg ggacaataat
     1141 atctccaaca gatggtatca aatttgaaaa aagagatatc ttggttttac atgggaaaat
     1201 atatgctatt ggattgaacc ttaaagagaa aataagaaaa gaaaaattac ttacaaataa
     1261 agagttcgag ataataaatg ccgataatat gtatatttcc ccaggattta tagatgttca
     1321 cacccattgt tataagagaa aattacctac aggtatggat tctgattcaa ttggaattga
     1381 aaaaggttct acagttatat ttgatgcagg tacagctggt ccttcaaact actatgattt
     1441 taaaaaaaag tatatggatg agtgtaaaac agaagtatat tcttttttaa atgttacaaa
     1501 tgagggacta aatatattga atgaatcaac ttctttagat gttataaatt ttgacaaggt
     1561 tgagtcagtt gtagaaaaaa atatagaaaa gataaagggt ataaaagtaa aagcgagtaa
     1621 atttcaatct aatgaaagtg gtatagatat aattaagaaa tcaaagcaag ttgctaaaaa
     1681 attaaattta cccttagtgg tatatatagg agagtatcca tcatatatag atgaagtaat
     1741 gaatattttg ggaaaaggag atgtggttac accagtatat ggaaatagta caaatggaat
     1801 ccttaaagaa tctggcacat taagaaaatc agtaataaat gcaaagaata gaggagtatt
     1861 gttcgatgta tgtcatggag tggaccaatt tgattttaaa gtttttaaaa aagcaataaa
     1921 gcaaggttta gagcctgacc taataagtac tgatatgcat ataaaaaaca tgaaagacgt
     1981 aaactattct cttttaaatg ttataaataa aattatggaa cttgggatat ccctagaaaa
     2041 gtgtatagaa aaagtaactg aatacccaac aaagattttc gatttacatg gaaataaagg
     2101 cagaataaag attggtggag aaggtgattt tacaatattc acaatggagg agtgtaatga
     2161 tattataaaa gactacaata ataatgaact tgttttaaat aaaaaattaa gattgcaata
     2221 tactgtaaaa tcatggatga aaagcagtga agtttataga catggatacg aaaatactat
     2281 aataaactaa acaatagtga taaaataata aatatatgtt taaattggta tatattggat
     2341 aaatatctaa ggaaacaaca attaaaaaat aataatttta ggggaggtat tttagtatga
     2401 aagcatttgt tgataaggat gtatgtgtag cttgtggagc atgtatagga acatgtccag
     2461 acgtttttag tatgagcgat gaagggcatt cagtagcaat tgaaggagaa ataccagcag
     2521 atttacaaga tttatgtaaa gaagccgaag aaggatgtcc agtttcagct ataacagtta
     2581 aatagagctg tttaaattta tttttaaaat aaatatataa gatttaattg gtacttaagc
     2641 tatagtatta gaaatttcta ttactatagc tttttaaaat agataaactt aatatataag
     2701 atattacttt ttacaaatat ggaggactga aatatgaaaa aaaattatga aatagtatta
     2761 ttcgatttag atggaacact tacagaccct aaagaaggaa taacaaagtc tattcagtat
     2821 tctttaaata gttttggcat aaaagaagat ttggaaaact tagaccaatt tataggacca
     2881 ccattacatg atactttcaa ggagtattat aaatttgaag ataaaaaagc taaagaagct
     2941 gttgaaaagt atagagaata ttttgctgat aaagggatat ttgaaaataa aatttatgag
     3001 aatatgaaag agatattaga aatgctctat aaaaatggta aaatattatt agtagcaact
     3061 tctaagccaa cagtttttgc agaaactata cttagatatt ttgatataga tagatatttt
     3121 aaatatatag caggtagcaa tcttgatggt acaagagtga ataaaaatga agttatacaa
     3181 tatgtactag acctatgtaa cgtaaaagat aaagataaag ttattatggt tggagataga
     3241 aagtatgaca taattggagc taaaaaaata ggtatagatt ctattggagt attgtatggg
     3301 tatggttctt ttgaagagat tagtgaaagt gaacctactt atattgtcga aaatgtagag
     3361 tctataaaag atattttgtt ataaatatct tttatagaca aaaatattac aaaatggtgt
     3421 cgcatattta aaaatatgat attatagtta aggaagtcat ttaaaaaaga ggaggatgca
     3481 cttaaaatga aatctatttt aattatgggt ggaagcgact tcataggttc tgcacttgct
     3541 aagcgcttga ttaaatgtgg gtatcaaatt gatatactta caaatggtaa aaaggaaata
     3601 gattataatg gttttaaaga acatctaatt tgtgatagaa aagttcgaaa agatatggaa
     3661 aacattatca caggaagaaa atatgattac atatatgata tgacagcata tacaaaagaa
     3721 gatgtaagta atctgattga ttttataagc atggataact taaaaaagta tatagtattg
     3781 tcagcaggag cagtatataa agatagtgga agaaatataa aagaagaaaa tgagaaaggt
     3841 gaaaatgaaa actggggaaa atatgggtta aataaaaaag aggctgaaga ttttattata
     3901 aatagcccta taccatatat aattattaga ccaacttaca tatatggtga aaataataat
     3961 ttatatagag agtattattt ctttgaaaaa atagaaaaaa atgaaaagat accagtacca
     4021 aaaggaaaac aagttagtaa tcaatttata tatattgggg atttggtaaa ggtattggaa
     4081 agcataatga aaaatcctca tgtaagagag gcctataatg tgacaaatcc acagttaatt
     4141 tcttgggatg accttatata tacatgtgga gaaattattg gcaaagaacc tataataaaa
     4201 tatgtagata tggaaaaagt agagtttaga gaaagaacgt atttcccatt tagaaatata
     4261 gactttaatc ttgatataaa taagcttatt gaacatgggc tttatatacc aaacgtgtta
     4321 ctaaaagaag gtcttacagc aacatataaa tggttttcag caaataagcc aaaaatgcat
     4381 gacagaaaaa tgaacaaagt tgagtctgta atgcaaatag tttaaaaaaa taaataagta
     4441 ggtagatatt ttgaactcaa aaattaagta ttttttaaaa ttttttatct taatatttat
     4501 tttgattttt atatactcag ttgcgataga gccgagtcta ttaattgtga aaaaatataa
     4561 tataagtgaa aatggagcat tgataaaaaa tgatacaatt gagagtaaat ctgaaagtgt
     4621 aaaagtagtt caaatttcag atactcaaat aggtagtttt tattcaacta agaatctcaa
     4681 aaaagtagct aataagatta acactttgaa tccagatata a
//
LOCUS       DAGSHT010000239         4718 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.245, whole genome shotgun sequence.
ACCESSION   DAGSHT010000239 DAGSHT010000000
VERSION     DAGSHT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.245"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            120..626
                     /locus_tag="MJB72_003334"
     CDS             120..626
                     /locus_tag="MJB72_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBY8264397.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1039..1707
                     /locus_tag="MJB72_003335"
     CDS             1039..1707
                     /locus_tag="MJB72_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264398.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1866..2372
                     /locus_tag="MJB72_003336"
     CDS             1866..2372
                     /locus_tag="MJB72_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBY8264399.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2500..3516
                     /locus_tag="MJB72_003337"
     CDS             2500..3516
                     /locus_tag="MJB72_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBY8264400.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3870..4574
                     /locus_tag="MJB72_003338"
     CDS             3870..4574
                     /locus_tag="MJB72_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBY8264401.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         2001 a          407 c          806 g         1504 t
ORIGIN      
        1 tgtattataa tttacatgaa ttatgtagga caatcaaaag tcagtattat aagagtacat
       61 atattcaaat ttttaacaat taatttatat aattaaaaaa tgaagggtag gatgaagtca
      121 tgggttttag agaagtaaaa attgaggaat tacagtttaa tccatttact aagattggca
      181 aagaatggtt acttataaca gctggtgatt cagaaaagtt taacacaatg actgctagct
      241 ggggtggtgt tggagtctat tggggtaaaa atgttgttac aacttacata agaccacaga
      301 gatatacaaa agaatttgta gatagtaatg atacatttac agttgccttc tttgatgaaa
      361 cgtatagaga ggctttaaat atttgtggta ctatttctgg tagagatata aataaaatag
      421 aaaaagcagg acttacacca tattttgtgg atgatacagt agcttttgaa gaagctaata
      481 tgattatagt atgtaaaaag ctatatcatg gtaatatgcc accagaaaac tttgatgcta
      541 aagaaaatga taaaaaatgg tatccagaga aagactacca tactatgtac atttcagaaa
      601 ttataaaagt tcttgtaaaa gaataattat aataaaaaat atatgaaaag aacttattat
      661 aagtaaatga ctgtgctttc ttatctgata tctaagtaga tgctagtaac taaataaata
      721 ttaattttaa ataaatacta ttataaaaaa gatgaactat atttactata gttcatcttt
      781 ttatttatat aagcacttat ataagtagag atttatttga ataaaagtat ttatttgaaa
      841 tatttgagta tactttataa gtatttttat gctaactaga aacacttaat ttatggcatt
      901 tgtatttgat ttattttaat gtaaataatt tcaaagttaa ttttattcaa tatagtacta
      961 taaaagtata aataacattt aataaaaata tatattatat taggtatttt aaataaattt
     1021 tttaaagggt gattttttat ggaaaatata actagttatt ttacaacaat actatgtgta
     1081 tttatatgct tatcatctgt atttatattt actcaactag ctagagtatt tattaataag
     1141 aagaagatta atcaaaaaat taaatcaaga aacggattta ggtatgatag agattttatt
     1201 gaagccagaa gagaagaaat acatataaag gataacaata ataataaaaa taagagtaac
     1261 aataagaaat taaagggaga aaaagtattt aaatacgata atggtgattt atataaaggt
     1321 gagtttgttg atggaaagaa aaatggattt gggatatata ttttttcaag taaggaaaag
     1381 tatgaaggat tatggaaaga tgacaagatg catggaatag gaaaatatac ttatagagat
     1441 ggttcaatat atacaggtga atttaaatac ggattaaaaa atggtttggg aaaattaaca
     1501 tatcccaata atgacattta taagggatat tttttagata ataagaaaaa tggtaagggt
     1561 gtattatata aaaatgatgg aaataaacaa gcaggaatat gggaagatga tgaacaatgt
     1621 aaaagtttag attttaaaga tttaaataat aataaaataa ataatgttta tcaaaataga
     1681 ttaaataaca ataaattaaa agtttaatta ttctaataaa aaaatcttta tttatgatgg
     1741 aatataggat atatctatgt tgtaatttgt aaaatcttcc tatatcatat aaaattaagt
     1801 attgattagt ctattacata aatatagaat ataaatatat ccatttaata ataaggagaa
     1861 gagatatgaa agagtatgta gttgtggatt tagaaactac aggattagat ccatataaag
     1921 gttgtgaaat aatagaaata ggaataacag aaataaaaaa tgaacaaatt gtaaaaaact
     1981 attcaaggtt gataaaacct aaggggataa tttcttcatt tataacagaa ttgacacata
     2041 taagtaatga aatggtagaa aatgaagaac cactagaatt agttttacct agatttagaa
     2101 aatacatagg tgatagaact ataattgcac acaatgcgaa atttgattta aaatttttaa
     2161 attactatct tagaatgtta gatttagaac caataaacaa ttatatatgt acagtagagt
     2221 tattaaaaaa atgtaaaagt tataaaggaa aaaacaagaa acttgaaaca gcttgtgctt
     2281 attacaacat agagaatata aatgctcata gagctgacag tgatacatta gcaactgcaa
     2341 aactattttt gaaaataaaa gatgaatatt aattataaat atagagaaaa ttagtgaaaa
     2401 tatcctttat aaaattgacc cttaagctta caattttgtt ctttgattta ttttgtagaa
     2461 caatgtataa tgtaccttat atgaagaagg gagactaaaa tgggttatta caatgataat
     2521 gataataata aaataagacg taaaaaaatt agtaacagca aagatagtaa taaaaaatct
     2581 aaacattctt atactccggc aaataaaaaa aataaaagtt ctaagacaag ttctagtaaa
     2641 agaatgagtg aaaaagtaga ttcacagaat cttataaaaa gagatagaga tagttttaca
     2701 agagataatg taagaccttt aaatacagat tacaatcaaa gagaaagaag aagagtcgag
     2761 tataaacaaa gagagaaaaa attaaaaagt aaaaaaagaa aaaaacaaaa aaggattaga
     2821 atagttagag cattattatc tattatgtta gttgcactaa ttatattttc agtggcaggt
     2881 gcaatattta cagtttcagc tataaaaggg tcaccagaag ttactaaaaa gttaattagg
     2941 tctaaatata taagtagtga ggttgtaagt ataaaacaaa tgccaaatga tttaaaacat
     3001 gcaatagttt ccatagaaga tgagagattt tataagcata aaggagtaga tgttatttct
     3061 ctagttaggt cagtattaaa caatgtgttt acagatacaa cacaaggtgc aagtactata
     3121 gatatgcaag tttcaaaaaa cctgcttact agtaatgata aaactatgaa gcggaaaata
     3181 agggatatgt ataatgctaa gcagatgaat aagattatga ctaaggatga aattcttgaa
     3241 gcatatttaa ataatatgta tctaggtaaa aatgcatatg gagctgcaaa gggagctgaa
     3301 gtctactttg gtaaaaaagt tagtgaatta aatttagctg agtgtgctat gttagctggt
     3361 atgacaaata atcctgcaag gtatattgac catggagagg ctaagaagag acaagtgatt
     3421 gtactgtata agatgcatca gcttggttat ataactgata aggagtatag aatagcaaaa
     3481 gctgatgaca ctccatttaa atcagaaatt gattaaaatt gtaagaatat agtattttca
     3541 atagtttaaa caaacaaaaa tgtaataaaa aaggtgattt gccacccatt tgccaccata
     3601 attataatct aatggcaaat tgttttaatg tacaaactag ataatagaat attgtcttaa
     3661 atgctatatt atgctaaaat ttaattgtat tttgtaaatt aaaaaactta taatttagtt
     3721 ttctagggtt ccgcatttta taatggtcag gtccaagaga aaacgcgcaa ttgttgtgta
     3781 cacggaagga taaaagcctg ggagatattt aagaatattt ctataggctt ttttattttt
     3841 acatatttta aatttgagga ggttttctaa tgaaaaaatt tgttattgag gatgcatttt
     3901 ggaatctatt tcctagtgca aaaattggag ttgttatttg taatgatata gataactcta
     3961 taaaagatga agactataag aaaataattt tgaagggaga agaagaagca ttaaaatact
     4021 tggaggactc agaatttagc aacaaccaag tgattaaagt atggagagaa gcatttaaaa
     4081 aatttaaaac gaaaaaagga gcaaggtctt ctattgaagc attattaaaa agagttcata
     4141 atggaaataa tataggaact attaaccctt tagtggatat ctacaatttt atctcattaa
     4201 aatatgcatt accttgtgga ggggaggata tagataaatt tataggagat attaggctaa
     4261 ctagagcaat tggtaatgag gagtttatcc cactagggac agatgaaaat tcatttccat
     4321 atgaaggaga aataatttat aaagatgatg gtggagcaat ttgtagatgt tggaattggc
     4381 gtgaagcagt gagaacaatg cttactgaag atactaacaa tgccttttta tgcattgaat
     4441 tagtagatga aagtagattt aaagaatttg aaaatgcttt aaatgaatta gctaaaatag
     4501 ttcaagaaaa attgggtggg aattgtaaaa tttcaatttt agatattgat aataaagagg
     4561 tatcagtgga ttaatatgga ggtgctgttg taatggacta ttctagttat aaagatactt
     4621 atatttaggg taagaaggtc tattttagat ataacgatta tcaaaggata taatatagaa
     4681 aataaaaatg tagaatataa aaagccttta aaataaga
//
LOCUS       DAGSHT010000240         4691 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.246, whole genome shotgun sequence.
ACCESSION   DAGSHT010000240 DAGSHT010000000
VERSION     DAGSHT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.246"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(38..403)
                     /locus_tag="MJB72_003339"
     CDS             complement(38..403)
                     /locus_tag="MJB72_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264402.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            868..1653
                     /locus_tag="MJB72_003340"
     CDS             868..1653
                     /locus_tag="MJB72_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264403.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLQGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(1750..2574)
                     /gene="pdaA"
                     /locus_tag="MJB72_003341"
     CDS             complement(1750..2574)
                     /gene="pdaA"
                     /locus_tag="MJB72_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBY8264404.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            2795..4684
                     /locus_tag="MJB72_003342"
                     /pseudo
     CDS             2795..4684
                     /locus_tag="MJB72_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1908 a          443 c          707 g         1633 t
ORIGIN      
        1 aaaaatgtaa attacttact ttttggtatg tataggatta cttattgtag caaattcctt
       61 tattatctag aatttcttgt tgaccatgtt caagcatata ttcgattcct atttcttgca
      121 ttatttccac tgcttttaac atttttttac cacgattagt aagaaaatat tcaacatgta
      181 atggatatcc agcagaactt attttatcaa taatcccaaa atcacgtaac tcttttaatt
      241 gttgaatcaa cattttttga tttattccat aaatattatg ttgcaattca gaaaatgttt
      301 tattagattg tcttaattca aatataagga tagttttcca ttttcctttg attatgtcat
      361 gtacaatttc aagaggacat gtatattcag ctcttatttt catagcatat cacccccagt
      421 aaaatgataa taaattatat cacttgtctt gtgatatgac aaatatatat gaactgtata
      481 taaattttat gttttattaa gttctaaatt tcttctattg tagttcatat ttagtacata
      541 aataattagt aagatataag taaataaata tataacatga ctatagaaat ttaactttaa
      601 aataacttat gataatttag aggaggaata tatatgaata tatcaagaaa agctatgaaa
      661 ataatagaat tagcacaaaa aattgcaaat aagagaggta tttcagtaga agaagcttgg
      721 agtgaagcag taacagaata taaaaataaa tacgaacata ttgcatagat atttaaaaat
      781 ttagaatatt tggtagaata taatgatttt aaatgatact ataaattata aggtatatca
      841 ttattaacat agaaggagga tatctgcttg attgtaaaaa tagataatac tttagaaaaa
      901 gaattttggc aatatgtatc acatgaagaa agtttaaatt tattcataat tggatacgtt
      961 gagaattatg gatttagctc tcaatatcaa gatatatggt ctcaggtaga aggtggaaat
     1021 ataacctcta taatattaaa aaataagtca acacttataa tttatagttt taaaaataat
     1081 tttaatatag gagaaatgaa gaatcatata aaagatttag atgtagaaag cataagtgga
     1141 aaaaaatgtg taatagatag actaataagt aaatacaaag atttttatga aaaactagat
     1201 aataaatttt gtgtattaaa agaaataaaa gaaatagatt tttccaacat gaaggagtat
     1261 aaaatagaaa atgctcagga aaaagatatt gatgaaatag gaaaactttt aaataggtca
     1321 gactataaag taagtaaaaa ttacatagaa gaaagaaaag ttcatttaaa agagggaaat
     1381 gtaagagcat attttattag aaatgatgat actatgatta gcactgtgtc aactggtatg
     1441 gaaactagtt ttttggcaat ggtggtctct gtaagtacag ataaaagata taggggaaaa
     1501 ggacttgcaa gttatatggt ttacaatttg agtaaagaat tgttgttaca aggaaaagtt
     1561 ccatgtcttt tttataataa tgatgtagca ggtaaaatat atcataatat aggatacaaa
     1621 gagataaatg aatggacaat tctttttaaa tagctataaa aaataaaaaa ctctatcgag
     1681 aattaaagta tttataaata actatatttt tactataatt catgatagag tttttttaaa
     1741 tttatgatac tattttttat tatttaaata atcaagtgtt ttaagtttat aaccttcttt
     1801 ttcccaatgt gtaagaacct catctaatat ctcagtattt gttgatgaat ttggatgcag
     1861 taaaactatt gcaccaggat gagttcttga ataaattttg tttttggcaa attcatgagt
     1921 aggttgtttc ttttcatacc aatctacata agcaaaactc caaaatattg atttatatcc
     1981 taaatcttgt gtatatttta aagattgttc gctaaattta cccattggag gtctaaaata
     2041 cttaggcatt tcttttccag taacatcttt gtatgctttt tctacacttg taatttcttc
     2101 tttgaatttt tcaaaatctg taatacctgc cattgatgga tggctttttg aatgattaca
     2161 aactaaatgt ccttcttttt ccatacgctt tattaactct ggattgcttt ttatataact
     2221 ttcaactacg aaaaattgag ctttagcatt gtgtttttta agcgtatcta ataattttgg
     2281 agtgtttcca ctttcatatc cagcatcaaa tgatagatat ataacttttt cattgggatt
     2341 tcctacataa taagctccgt attttttaaa aaaatcagct tcttttatag gtgatggctg
     2401 tttaccatct tccctagggt taaaatacca atcatattca tgtgtgtcga gtgttggact
     2461 agaaatttga gtttttgttt tatctaatga tttaaaattt atactagcaa ttccaaaaag
     2521 tgttagagca aatgctccta tcataatgta tttttttagg ctatcttttt tcacaatttc
     2581 acctcttatt taaaatttaa agtatatttc ttataatata gtttgaatat ttatagttaa
     2641 aaatattcac atatacaaat aataaatgaa agaagtttac tgtaagatat aaagttgata
     2701 ttaattatct taaataaagt gtatataaat gataaaatat aaagagaaat gattgtaaat
     2761 aagttaggct ggtttaactt gaagggatat aaaaatggat aatataataa gtgtattgaa
     2821 tgatgttgtt tttttagata tagaagttag tggattagat tgtttaaatt cagaaatttt
     2881 agaagttgga gctgtcaagg ttaaagactg gaaaatatat acctatgaat cattaataaa
     2941 aaacaaattt gaggtaccta tagaagtatt ctcagtatgt aaaaatttag ataaaaatga
     3001 tttagaaata gctaataaaa tagaactggt ggaagataga cttgtaaatt ttgtagaaga
     3061 tagtttcatt atatgtcatg atttaagtct gaaaaaaaag ttttttgagt atcatatgcc
     3121 aaagttaaaa aataaattta ttgatttaat tgaattagca gttattttag aaccatatca
     3181 taaagattat agcttagaat acttaaaaaa tactttaaca aattgtaatt ctaaagtgga
     3241 aaatagagct ttatcagatg ctattgatat aataaatatt gtaaattgcc ttttagtaaa
     3301 atttaataac tatgaaaaaa ctactttgga gccattgagt tttaaaataa attcatatct
     3361 taaaaaattt aatcttccaa catgggaatg gagcaaattt ttagaagagg caaattatga
     3421 tttatcaaat aatataaata taaaaaaaga atataatata tttgactcaa aagaagaaaa
     3481 gaagaaggaa agagaaacct taaaaatttt aaatgaagaa gaaaaaaatt atgaagaatt
     3541 attaaaatac aaaactattt gggagaataa agaaggattt acatatgaat atagacctgg
     3601 tcaatatgag cttactaaaa caataagaga gttgtttaga aatagcgaag atgaagaaaa
     3661 aattgcatgt atagaggcac caactggtat aggtaaaagt gtaggatatt tattgcctgc
     3721 aatattagag gctagaatta ataaaaaaag attattaatt tctactgaca caaaggaatt
     3781 gcagatacaa ttaataaata aagatattcc aaatgtgtta gattctttgg gtctaaatgg
     3841 aaaagtaagt tacggatata taaagggaaa aaataactat atttgtatag atagactgga
     3901 agcatatata gatgattatg agtctcaaaa ccctactaaa ggagaaattt tatctttaat
     3961 atttttgaag agattagttg aaggtggcaa gtatggagat atagaagaaa taaattattt
     4021 agtgtttgac aacttcaagg aaataagtac tcacttaaga aatgtaagtt gtgacccaaa
     4081 tatgtgcaga cctaaaaaat gcaaaaaaga ttgtttatat aaaaacagaa tagaagaatt
     4141 aaaagaagaa catataacag tagtaaatca ttcactatta gctaaatggc cgtataagga
     4201 tgaaaaacct ttagaaaata taatagtaga tgaagcacat aatttgactg aaaaaggata
     4261 tgatttcttt tcaagtatta taaattcaaa gtctttaagg tacttattac aagaaattta
     4321 tccttacgag tttattcaaa atagtagctt tatatataaa aaatatagta gaaatatgag
     4381 aaaaataaag gcttttgata aattttataa tgtattaaaa attggaagag aagataaaca
     4441 aaaaattgct agaagtataa atttcattat tgaagagata gattcaatat taaagtttgg
     4501 taattgtaat gaatataata atgtaagtaa ttataattta agatgggaac tgaatttgca
     4561 aatagatgaa attgtgggaa aactaaaaaa agatggtata gatactgaaa taagttatag
     4621 agcctatagt gaaaagataa agttgagttg tgagaaaata ataaaaaatt tagtttcaat
     4681 aatataataa t
//
LOCUS       DAGSHT010000241         4674 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.247, whole genome shotgun sequence.
ACCESSION   DAGSHT010000241 DAGSHT010000000
VERSION     DAGSHT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.247"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..253
                     /gene="tcdR"
                     /locus_tag="MJB72_003343"
     CDS             <1..253
                     /gene="tcdR"
                     /locus_tag="MJB72_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBY8264405.1"
                     /translation="ENILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLK
                     GYSDREISVKLKISRQAVNKAKNRAFKKIKKDYENYFNL"
     gene            571..>4674
                     /gene="tcdB"
                     /locus_tag="MJB72_003344"
                     /gene_synonym="toxB"
     CDS             571..>4674
                     /gene="tcdB"
                     /locus_tag="MJB72_003344"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBY8264406.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLI"
BASE COUNT         1907 a          481 c          777 g         1509 t
ORIGIN      
        1 tgaaaatatc ttaaatatga aaaataatta tcaaagtaag tctgtttttg aggaagatat
       61 ttgttttttc gaatatattt tgaaagaatt atctggtatt caaagaaaag ttatttttta
      121 taaatattta aaaggatatt ctgatagaga aatatcagtg aaattaaaaa tatctagaca
      181 agctgttaat aaggctaaaa atagagcatt taaaaaaata aaaaaagact atgaaaatta
      241 ttttaacttg taattaatga gcttaaagaa atatttacaa tagaaatcaa attttagaat
      301 taactttatt gtaaaatcaa taacttaatc taagaatatc ttaattttta tattttatat
      361 agaacaaagt ttacatattt atttcagaca acgtctttat tcaatcgaag agcaaattaa
      421 tcaactgagt gtcttaaatt taaaatgtta ggaagtgaat gtatatgaaa acctaagtag
      481 atattagtat attttataaa tagaaaggag gatatataaa agagttttag catttagatg
      541 taaaaatatt atagtaaagg agaaaatttt atgagtttag ttaatagaaa acagttagaa
      601 aaaatggcaa atgtaagatt tcgtactcaa gaagatgaat atgttgcaat attggatgct
      661 ttagaagaat atcataatat gtcagagaat actgtagtcg aaaaatattt aaaattaaaa
      721 gatataaata gtttaacaga tatttatata gatacatata aaaaatctgg tagaaataaa
      781 gccttaaaaa aatttaagga atatctagtt acagaagtat tagagctaaa gaataataat
      841 ttaactccag ttgagaaaaa tttacatttt gtttggattg gaggtcaaat aaatgacact
      901 gctattaatt atataaatca atggaaagat gtaaatagtg attataatgt taatgttttt
      961 tatgatagta atgcattttt gataaacaca ttgaaaaaaa ctgtagtaga atcagcaata
     1021 aatgatacac ttgaatcatt tagagaaaac ttaaatgacc ctagatttga ctataataaa
     1081 ttcttcagaa aacgtatgga aataatttat gataaacaga aaaatttcat aaactactat
     1141 aaagctcaaa gagaagaaaa tcctgaactt ataattgatg atattgtaaa gacatatctt
     1201 tcaaatgagt attcaaagga gatagatgaa cttaatacct atattgaaga atccttaaat
     1261 aaaattacac agaatagtgg aaatgatgtt agaaactttg aagaatttaa aaatggagag
     1321 tcattcaact tatatgaaca agagttggta gaaaggtgga atttagctgc tgcttctgac
     1381 atattaagaa tatctgcatt aaaagaaatt ggtggtatgt atttagatgt tgatatgtta
     1441 ccaggaatac aaccagactt atttgagtct atagagaaac ctagttcagt aacagtggat
     1501 ttttgggaaa tgacaaagtt agaagctata atgaaataca aagaatatat accagaatat
     1561 acctcagaac attttgacat gttagacgaa gaagttcaaa gtagttttga atctgttcta
     1621 gcttctaagt cagataaatc agaaatattc tcatcacttg gtgatatgga ggcatcacca
     1681 ctagaagtta aaattgcatt taatagtaag ggtattataa atcaagggct aatttctgtg
     1741 aaagactcat attgtagcaa tttaatagta aaacaaatcg agaatagata taaaatattg
     1801 aataatagtt taaatccagc tattagcgag gataatgatt ttaatactac aacgaatacc
     1861 tttattgata gtataatggc tgaagctaat gcagataatg gtagatttat gatggaacta
     1921 ggaaagtatt taagagttgg tttcttccca gatgttaaaa ctactattaa cttaagtggc
     1981 cctgaagcat atgcggcagc ttatcaagat ttattaatgt ttaaagaagg cagtatgaat
     2041 atccatttga tagaagctga tttaagaaac tttgaaatct ctaaaactaa tatttctcaa
     2101 tcaactgaac aagaaatggc tagcttatgg tcatttgacg atgcaagagc taaagctcaa
     2161 tttgaagaat ataaaaggaa ttattttgaa ggttctcttg gtgaagatga taatcttgat
     2221 ttttctcaaa atatagtagt tgacaaggag tatcttttag aaaaaatatc ttcattagca
     2281 agaagttcag agagaggata tatacactat attgttcagt tacaaggaga taaaattagt
     2341 tatgaagcag catgtaactt atttgcaaag actccttatg atagtgtact atttcagaaa
     2401 aatatagaag attcagaaat tgcatattat tataatcctg gagatggtga aatacaagaa
     2461 atagacaagt ataaaattcc aagtataatt tctgatagac ctaagattaa attaacattt
     2521 attggtcatg gtaaagatga atttaatact gatatatttg caggttttga tgtagattca
     2581 ttatccacag aaatagaagc agcaatagat ttagctaaag aggatatttc tcctaagtca
     2641 atagaaataa atttattagg atgtaatatg tttagctact ctatcaacgt agaggagact
     2701 tatcctggaa aattattact taaagttaaa gataaaatat cagaattaat gccatctata
     2761 agtcaagact ctattatagt aagtgcaaat caatatgaag ttagaataaa tagtgaagga
     2821 agaagagaat tattggatca ttctggtgaa tggataaata aagaagaaag tattataaag
     2881 gatatttcat caaaagaata tatatcattt aatcctaaag aaaataaaat tacagtaaaa
     2941 tctaaaaatt tacctgagct atctacatta ttacaagaaa ttagaaataa ttctaattca
     3001 agtgatattg aactagaaga aaaagtaatg ttaacagaat gtgagataaa tgttatttca
     3061 aatatagata cgcaaattgt tgaggaaagg attgaagaag ctaagaattt aacttctgac
     3121 tctattaatt atataaaaga tgaatttaaa ctaatagaat ctatttctga tgcactatgt
     3181 gacttaaaac aacagaatga attagaagat tctcatttta tatcttttga ggacatatca
     3241 gagactgatg agggatttag tataagattt attaataaag aaactggaga atctatattt
     3301 gtagaaactg aaaaaacaat attctctgaa tatgctaatc atataactga agagatttct
     3361 aagataaaag gtactatatt tgatactgta aatggtaagt tagtaaaaaa agtaaattta
     3421 gatactacac acgaagtaaa tactttaaat gctgcatttt ttatacaatc attaatagaa
     3481 tataatagtt ctaaagaatc tcttagtaat ttaagtgtag caatgaaagt ccaagtttac
     3541 gctcaattat ttagtactgg tttaaatact attacagatg cagccaaagt tgttgaatta
     3601 gtatcaactg cattagatga aactatagac ttacttccta cattatctga aggattacct
     3661 ataattgcaa ctattataga tggtgtaagt ttaggtgcag caatcaaaga gctaagtgaa
     3721 acgagtgacc cattattaag acaagaaata gaagctaaga taggtataat ggcagtaaat
     3781 ttaacaacag ctacaactgc aatcattact tcatctttgg gggtagctag tggatttagt
     3841 atacttttag ttcctttagc aggaatttca gcaggtatac caagcttagt aaacaatgaa
     3901 cttgtacttc gagataaggc aacaaaggtt gtagattatt ttaaacatgt ttcattagtt
     3961 gaaactgaag gagtatttac tttattagat gataaaataa tgatgccaca agatgattta
     4021 gtgatatcag aaatagattt taataataat tcaatagttt taggtaaatg tgaaatctgg
     4081 agaatggaag gtggttcagg tcatactgta actgatgata tagatcactt cttttcagca
     4141 ccatcaataa catatagaga gccacactta tctatatatg acgtattgga agtacaaaaa
     4201 gaagaacttg atttgtcaaa agatttaatg gtattaccta atgctccaaa tagagtattt
     4261 gcttgggaaa caggatggac accaggttta agaagcttag aaaatgatgg cacaaaactg
     4321 ttagaccgta taagagataa ctatgaaggt gagttttatt ggagatattt tgcttttata
     4381 gctgatgctt taataacaac attaaaacca agatatgaag atactaatat aagaataaat
     4441 ttagatagta atactagaag ttttatagtt ccaataataa ctacagaata tataagagaa
     4501 aaattatcat attctttcta tggttcagga ggaacttatg cattgtctct ttctcaatat
     4561 aatatgggta taaatataga attaagtgaa agtgatgttt ggattataga tgttgataat
     4621 gttgtgagag atgtaactat agaatctgat aaaattaaaa aaggtgattt aata
//
LOCUS       DAGSHT010000242         4612 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.248, whole genome shotgun sequence.
ACCESSION   DAGSHT010000242 DAGSHT010000000
VERSION     DAGSHT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.248"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            78..416
                     /locus_tag="MJB72_003345"
     CDS             78..416
                     /locus_tag="MJB72_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBY8264407.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(823..2259)
                     /gene="miaB"
                     /locus_tag="MJB72_003346"
     CDS             complement(823..2259)
                     /gene="miaB"
                     /locus_tag="MJB72_003346"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBY8264408.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPD
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(2507..3943)
                     /locus_tag="MJB72_003347"
     CDS             complement(2507..3943)
                     /locus_tag="MJB72_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBY8264409.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNTYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1519 a          699 c          473 g         1921 t
ORIGIN      
        1 aaatgatttt aatgtgcctt tatggtaaaa ttactaataa gtaataaaat taattaaaat
       61 atatggaggt gcttataatg tttgctgtag gatttgttgt tataattgca cttgcctttt
      121 tcattttaag tgtactatta tttaatggaa aagccagtat gctactagca ggttataact
      181 ctatgagtaa gaaagaaaaa gcacgatacg ataaaaaaaa attatgtaga agttctagca
      241 ttgttacttt tattgtctct ataatgctat ttattatggc gtttttggga tatagaatag
      301 agagtggtca aatggacgaa aaaaaaatgg ttcccttttc tataatattt ataatagtca
      361 tactttccag tgtatttttt aatatcctct actctaacaa aaaatgtaaa aaataaatag
      421 ttgtaagttg agcagttaat tataaaagat tgactgcttt ttcatgtata ttgactactc
      481 ttattaaata ttttaacatt taaaaatcta tgtaaaaata atgaatatag tacaaacttc
      541 taataatgat tttacctttg tggttacagt ccactatata taatttaata tgataacagg
      601 gaattactaa tgacaactaa gtaaactttt ataaaatata ataaaacaaa aatagatgta
      661 caatgaaatt atatcttata tcaaatttat ttttattaat agcaaaaact tacattagca
      721 taacatatga caaattcgct taaaataaaa atttttaata taatttgttc tatagtacat
      781 ctattttgat tagtttacat aattttatta ttttgtacaa tattaattat ttactaatat
      841 cccatttaat gaaaaagttc taggttcagt aattttgacc ataactaatt taccaattaa
      901 atcatcattt ccaccttcga aatttacgag tttattttgt ctagttctac cagcaaactt
      961 attttcatta tttttacttc ttccttcaac tagaacttca actattctat ctttatatcc
     1021 atcatttatt tctgcagaaa tcctatttac agcttctaac accctattaa atctatcatg
     1081 ctttatatct tctggtattt gattttccat tttagcagca ggagttccct gtcttttaga
     1141 ataaataaat gtaaaagctg aatcatatct aactttttca accacatcta aagtgtccaa
     1201 taaatcttct tctgtttctc ctgggaaacc aatcattaag tctgtagaaa aagcaatatc
     1261 tggaacttcc tttttagctt tttctattat ttctaaataa tattcctttg tataatgtct
     1321 attcattttt ttcaaaaggc tactgcttcc acattgtatt ggtagatgta aaaactcgca
     1381 caccttatca caatctctaa ttgcgtatat tacttcatca gatatatctt ttggatgaga
     1441 tgtcataaat cgaacccttt caattccttc tatatcattc acttttctta aaagttctga
     1501 gaatgaaact ggattttcaa gtgtcttacc ataagaatct acattttgtc caagtaaagt
     1561 gatttctttc gttccatttg caacaagctc ttttatctcg ttaattatat cttcaggagt
     1621 tctacttctt tctcttcctc ttgtgtatgg gactatacaa tatgtgcaga aattattgca
     1681 tccatacatt atatttacaa atgcttttaa ttcaaatttt ctattacttc taaggccttc
     1741 tattacttct ccatccacat cccatacatc cactaacatt ttgtcagaat ttattgattc
     1801 tgtaagtaat tgcggaaatt tatatagatt atgtgttcca aatataaggt ctacatgttt
     1861 atattttttt cttaattcct ctacaacatg tggttgttgc atcatacatc cacatacagc
     1921 tattttcata tctggatttt ttccttttag atgttttaat tgtcctaagt taccatacac
     1981 ttttagctct gcattttctc taactgcaca ggtgttatat attattaaat cactttcttc
     2041 aaccatcatt gacatttggt atcccatctc ttctaacatt gaacataatt tttctgaatc
     2101 atgttcattc atttgacacc cgaaggtttg agtaaacacg agttttctct ttccagttct
     2161 gttgaagtat tcttcatttt gtctgtgtat ttgttctata tatttatctt gttcttgtat
     2221 tttctctata ggtattgtta cttgttttct tttgctcact tctattcctc ctagttaatt
     2281 tcttatatta cacataacct taattatatc aaataagaaa aatcttttaa agatattatt
     2341 atttttctag ttgtgtttta atagtaaatc taatgattta tatatactat atttctaaaa
     2401 tataaaatca tataattatt ataataataa aaagatgttt taaattaaaa cttaaaacac
     2461 ctttccttaa ataaaatata tgatttacta ttaaatacaa ttacatctat tcattttcaa
     2521 ttaaataaca atcatttaat attaaatatt gaccttcctc aaccgttata tatcttcttc
     2581 ctgaaaaagt atcattagta actattccct ctgcattacc taagctatca tttctaactt
     2641 cataatatgc atcagaagaa tttgacatta taatatattc tccagcagga atttctaatc
     2701 cgattttata cataccattt ccaaacttac cattcttggc cttctgggct ttagcatact
     2761 ttgctaatgt cattgttgaa tcctcaattt taatatattg accattcttt aaggtgatat
     2821 atctagtccc actaaaaata tcatttgaaa gtatactatc agcattacct gtactatcac
     2881 ttgtaacttc ataataagaa gcataacttc ctgagtttga agttattaag tattcacctg
     2941 ctgatatatc ttttccaacc ttatacattc catcttttat agtctttgta gtaggtttaa
     3001 cattttttac aaccccagta ttaatatctc caattccatt tgttatattt aaacactgtt
     3061 gtactatatt tgaatctata tatcctatag aagttatctt cttagcattt tttaaaatgc
     3121 ttttattgct accatcatta actaaaacca ttggaccatg ttttgataaa gaagatccta
     3181 caagtgcatt tgttaattca taagctccag ctatataaaa ctctctagca tccttataaa
     3241 acttatttat tattgcttta ttagtttcaa aacgagtact cccccctagt ctagtagatt
     3301 tagtattgtt tactaaagtg gtactcattg aagctgttcc tcctattaca taagacttta
     3361 aaccactagt tgaaaaaggt atactttgtc cattagtaag aattattggt gctttatctc
     3421 ttgctgctac agatgcaata cttatagcat ctgcttctcc tttatatgca ttggttagca
     3481 taacagtatt gacttttttt attgaattta tttcttttgc aacattataa cttgttttta
     3541 ttctgtcatt tcccttaatt cttacaactt tcattttctt agattttaat gaattttcga
     3601 cagatgtact aatagaatat gttcctccaa ttatataaac ttttgataca ttttttaatc
     3661 ttgcagatgt ctcagttggt atagtatttt tttcagttaa tagtataggg gcatttaaag
     3721 ctcctgctag tccacttgca cttaatccat ctgcaataga gttactagta ttaattaata
     3781 tagcagtttt atatgtattt ttatctgcta ttttacctgc tatttcatac ttattttttc
     3841 cttgtatttt ttctacagat gatattgcat ctactaattg aatatttgca aatacaaaag
     3901 aaatacttaa agtcaatgtt aatattcttt tatagaattt cataataaaa ttgtccccct
     3961 cataataaat ttttaatata taaatacata ttcttaaatt agttatgtac ttaacaaact
     4021 tatgtaaaat tgcaaattat attaagtata ttacaaataa atattaatac aattattcat
     4081 ttagctataa ttgtatttac atttatatta aaaatcaact agatttgtat aaatacttat
     4141 aattatattt ttattcttaa tatttttttt attatgttat tttaatagat ttatttttct
     4201 tttttaagca tagagatgta tttttccata tattctattt ctattatagt ttcgcttatt
     4261 taattcaccc aaaatttcac ttaatattag aattttaaat cttttactaa aacagacttt
     4321 tatagttcta ttaaaatttt ttactaaact ttatttttta ctatgaatta tctatttttt
     4381 ttggctaaat ttaagcaagt ttttcacaaa aaatatatac attactatat ttttaaaact
     4441 ttataaatat ataattgatt taattggaaa ttttagtgta ttcaaaaaat gagaaatcag
     4501 aggagattaa tataaaaatt tatcttgaaa agtcaattaa atatatctta tatttaataa
     4561 ctacaagtga gatacatata agaatattat ataggtatga taatttatcc gt
//
LOCUS       DAGSHT010000243         4597 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.249, whole genome shotgun sequence.
ACCESSION   DAGSHT010000243 DAGSHT010000000
VERSION     DAGSHT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.249"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1104
                     /locus_tag="MJB72_003348"
     CDS             <1..1104
                     /locus_tag="MJB72_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264410.1"
                     /translation="EFGDNLLITIAKALKEELTEGEVYARLSSDYFAIFCDYKNGRNK
                     IIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEGEVDIQKAVNKANMARSVAKGKNIN
                     YAIYNEDVRNKLSEESMILDDIKIALVKNQFEVYYQPKFSLVTGEMIGSEALIRWNHP
                     EHGFISPAVFIPIAEKSKLILKIGRFVFERVCNDLYEWKKQGKKIVPVSVNLSRVELY
                     QPDIVKFINKTIKMYNLSSDFIEIEITETVAINELNILKNVLNELRTYGFSISMDDFG
                     TGYSSISCLRDMPIDILKLDKSFLGGIEHDERSRNIAKSIVSLAKSLDLVVIIEGVES
                     KEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            1358..2020
                     /locus_tag="MJB72_003349"
     CDS             1358..2020
                     /locus_tag="MJB72_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBY8264411.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            2391..3800
                     /locus_tag="MJB72_003350"
     CDS             2391..3800
                     /locus_tag="MJB72_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBY8264412.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            3915..4190
                     /locus_tag="MJB72_003351"
     CDS             3915..4190
                     /locus_tag="MJB72_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBY8264413.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
BASE COUNT         1867 a          426 c          811 g         1493 t
ORIGIN      
        1 gaatttggtg acaatttatt aattacaata gcaaaagctt taaaagaaga attaacagaa
       61 ggtgaggtat atgcacgact ttcaagtgat tattttgcaa ttttctgtga ttataaaaat
      121 ggaagaaata aaattataag aaaacttgat aatatacgaa gtaatattga aagtaatttg
      181 agtatagtct ttgaaatttc tctgtgtgtg ggtatttatt ttgttgaaga aggtgaagta
      241 gatattcaaa aggctgtaaa taaagcaaat atggctaggt ctgttgctaa aggtaaaaat
      301 ataaattatg ctatttataa tgaagatgtt aggaataagc ttagtgagga atcaatgatt
      361 ttagatgata taaaaatagc attggtaaaa aatcaatttg aagtgtatta tcaacctaag
      421 ttttcacttg taactggaga aatgataggg agtgaagcac taataagatg gaatcatcca
      481 gaacatggtt ttataagtcc cgcagtattt attccaatag cagagaaaag taagttgatt
      541 ttaaaaattg ggagatttgt atttgaaagg gtttgcaatg atttatatga atggaaaaag
      601 caaggtaaaa agatagttcc agtttcagta aatttatcta gggtcgagtt gtatcaacca
      661 gatattgtaa aatttatcaa taaaacaata aaaatgtata atttaagttc agatttcatt
      721 gaaatagaga taacagaaac tgtggctatt aatgagttaa atattttaaa gaatgtttta
      781 aatgagttga ggacgtatgg attttctatt tctatggatg actttggtac aggatattct
      841 tctattagtt gtttaagaga tatgccaatt gatatattga agctagataa gtcttttctt
      901 ggtggcattg aacatgatga gagaagtcgc aatatagcaa aatcaatagt gtctctagca
      961 aaatcactag atttagttgt aattatagaa ggtgttgaga gtaaagaaca agctgagtta
     1021 atgaaacaat ttggatgtga tttagttcaa ggattttatt ttgcacgacc aatgccagct
     1081 aagaattttt tagatttact ttaaaacaaa atttgatttt tatataaagc actattatga
     1141 atgcttgatt ttaataaatc attgctaaat aattagtgtt tttttatttt atcaatgtat
     1201 tgtctaatat aaagcaaaaa ataattgtat tttataaaaa ttatattaaa aataaattta
     1261 ttttgctaat aaacatgtta ttaacaaaag aatagtacta taatgtatag atgataaaaa
     1321 ctttaataaa aatttagtgt ataggagttg tttaagtatg atagatataa gtattgatga
     1381 caaacttaag gaaagatggc ctaatgctaa gctgggttgc attcaagcaa aagtagcagt
     1441 aaataaaagc tcagaaaaat taattaatga aataaatgag ttttgtgata ctataaatca
     1501 aagtctaaaa gttgaagaca ttacgaaact agataaaatt aaagatgcaa gaaatgctta
     1561 taaagagctt ggaaaaaatc caagtagata tagaacatct tcagaagcat tggttagaag
     1621 aattattcaa gggaagggtc tttatacaat aaataatata gtagaaataa acaatttaat
     1681 atcaataaaa tcattatatc cagtaggtac atataatgta agcaaattac attcaccagt
     1741 atgttttact gtaggagatg aaggagaaca gtataagggt attgggaaag aattaataaa
     1801 tatagaaaat cttcctatat tatctgattc agtgggtaaa tttgggagtc caactagtga
     1861 ttctgaaaga gcaatgataa cagaagatgc aaatgaaata ttaatttgta tattttcttt
     1921 tagtcaagaa tctgatattg aagaatattt tggatatgct aaggaattac ttaaagaata
     1981 tgcagatgga aaagatatag aaactaaaat aattaaatag aatctatata acaacgttat
     2041 gttaagctaa aattcaatat aaaaataagt taagataaag aaactaacac aaaattagtt
     2101 tcttttttaa aatagtagtt tagtgttaca aaaatagata gatagtccca cgattattaa
     2161 aagtatggta atatattaat atagcagaga ttgtgtttaa gggattttga aaggggggat
     2221 ttataataat cattttatct aaaagataaa atggttttat agaaataact atatatttaa
     2281 aaatataaat aatgatacag taatttgggt atataaaata aggcatacat ggttttgttt
     2341 aaaaaccaat aaggtacata ggtaattaca atgtaaggaa ggatttaatt atgattgatg
     2401 gaaaagaggt aaaacaagaa agtggacaaa aaaatctaaa atttaagata gctatagtag
     2461 taggtgtttt gtttgtagct tatataggaa tggcaatctt ttttagaaat catttttact
     2521 ttggaacgac tattaatggc gtaaaggctt caggaaaaac agttgagcaa gtaaatgatt
     2581 tgttaacatc tagtactaat tcctatactt taaacttaga ggaaagaaac aataagaaag
     2641 aacaaataaa agcaaaagat attgatttaa aatttacacc agatgataaa gttcaaaaga
     2701 taaaagatag tcagagcgct attggatgga ttggtggaat atttaagtct aaagagtata
     2761 ataatatgat aactctttca tataataaag atttactaga aaaaaggttt aatgctttat
     2821 cttgttttga tagtaaaaat gttgtagaac ctaagagtgc ttatcctaag tatgatagca
     2881 ctaaaaacgc ttatgttata gtaccagagg tacttggaaa caaagtgaaa aaagatgatt
     2941 tttataagtt agtacaaaac tcaattaatt ctgggaatac ttctatttct ctggatgaaa
     3001 gtaaagttta tgaagcacca aacaacacaa aggattcaga aaagttgaaa caagctatta
     3061 agacattaga taagtatgtt ggtgtagtcg ttacatatga ttttagtgac cgtaaggaaa
     3121 ctttagatgg ttctgaaatt aataaatggt taaaagtaga ctcagaaaag aattatgaca
     3181 taacttttga taatgatgct atggtagaat atacaagagg tttaagtaaa aaatacagta
     3241 cttttggtga ctcaagacca tttaaaactg ccacaggtaa agatattaca atttcaggtg
     3301 gaatttatgg ttggttgata gataaaaaga aagaagctga agcattagtt gatgttgtaa
     3361 aagctggtaa aaatgttaca agagaaccta tttacataca aaaagctgta tctcgtaata
     3421 aagatgatat tggaaataca tatgtagaga ttagtctttc tggccaacat atgtggttct
     3481 ttaaaaatgg agaaatgctt gtatcaacag acgttgtaac aggtgattta gctagaggat
     3541 ttgctacacc atcaggtata tatcctctta attataaggc tagaaatgtt tcattaactg
     3601 gtcaaggtta cagttcacct gtaagttatt ggcttccgtt taatgggaac ataggcattc
     3661 atgatgctac ttggagaaat tcttttggtg gaagtatata taagtcttca ggttcacatg
     3721 gatgtgtaaa tacacctttt tctaaggcaa agaaaatata tgaaaatata gaaccaggta
     3781 caccaattat tttatactag taaatgtgtt gctaacagtt aagcgaagtc atcttagttt
     3841 atttaggatg gcttcctttt tatataaagc ttattgaagt ttacagaaag actacttaaa
     3901 aggagaaata cataatgaat gaaaaattaa ctaaaatttt agaagaagct aagaatgatg
     3961 atgaatttag aaaagaactt ataaaaacaa gagaacaacg tgatgttatg gattcatttt
     4021 gtaaggttgt tacatctaga ggttatgata tgacagtagg ggaacttttt tctatgggag
     4081 aagaatatac atccaatcta ctgaaaagtg taaatggagg agcagcatat cctattgaag
     4141 attggagtga ttggtacgag gattttttta taatgttgtc gaatatatag agtaactaat
     4201 gatattatct acttaagatt aatacattgc cttaaaataa aactattaga actaggtgta
     4261 agttttatca atctttaaaa atattgattt atttctaact agatataaaa aaattagatt
     4321 aaaaataagg tgacaaaaat cgatatagct tgatgattta taaaagttag ataaaatagt
     4381 gcgagatatt tctaaaaatt caaaaaaaac aataaacttc aatggttaga ggttgaaaaa
     4441 gaatataaaa aacttttata tgtaataaat taaattaaaa ttataaaaaa aataaaataa
     4501 aaatttaaaa tgtcgacata acgttaattt taaaacaaaa taacgctatt gacaaactat
     4561 atcgctttaa attataatta ttgaaaataa tggtatt
//
LOCUS       DAGSHT010000244         4591 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.250, whole genome shotgun sequence.
ACCESSION   DAGSHT010000244 DAGSHT010000000
VERSION     DAGSHT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.250"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..466
                     /locus_tag="MJB72_003352"
     CDS             <1..466
                     /locus_tag="MJB72_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBY8264414.1"
                     /translation="EVSKIDFHQVVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESI
                     KLGVLNSSKCDGYMFFVGDQPFIDNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFP
                     YSKKEELLALKEDEKGKTILKNSSKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            463..1482
                     /locus_tag="MJB72_003353"
     CDS             463..1482
                     /locus_tag="MJB72_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBY8264415.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            1820..3445
                     /locus_tag="MJB72_003354"
     CDS             1820..3445
                     /locus_tag="MJB72_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8264416.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            3644..4120
                     /locus_tag="MJB72_003355"
     CDS             3644..4120
                     /locus_tag="MJB72_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264417.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(4328..>4591)
                     /locus_tag="MJB72_003356"
     CDS             complement(4328..>4591)
                     /locus_tag="MJB72_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY8264418.1"
                     /translation="CEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVP
                     IKVISERVGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         1826 a          445 c          850 g         1470 t
ORIGIN      
        1 agaagttagc aagattgatt ttcatcaagt tgtagtagta agtcaatatg aagaggtttt
       61 gttattgtgt aataaatatg gttttgaata tatagataat aaaaatgcaa atataggtca
      121 aagtgaatct ataaaattag gagttttaaa tagctcaaaa tgtgatggtt atatgttctt
      181 tgtaggagac cagccattta ttgataactt atatatagat aaaattataa atacttttaa
      241 attagataga gattttatag taattccaag atacaaagat atgtgtggaa atccagtcat
      301 attcccatat agcaagaaag aagaattgtt ggcacttaaa gaagatgaaa aaggtaaaac
      361 cattttaaaa aattcatcaa aaataaagta tgtagaagtt cctaaagaaa tgttatttga
      421 tatcgataca aaaatggact atgagagaat tgggggtaaa ttatgaagct aataaaaact
      481 atagatgctg ttggacaagt tttatgtcac gacataactc aaataatacc tggagaattt
      541 aaaggaagaa aatttaaaaa aggccacata gtaaaagaag aagatattcc agtgttatta
      601 tctcttggta aagataattt gtatgtatgg gaaaagtcag aaggaatggt tcatgaaaat
      661 gaaggcgcat tgtttttaaa agaactaact gctggagaaa atttagattt tagtgaaata
      721 aaagaaggga aaatagattt tatagcagca tgtgatggtc tattaaagat agatgttgat
      781 gcactttttg atttgaattg tgttggagaa ataatgatgg ctactcttca taataatttt
      841 atagtaaata aaggtttaaa agtagctgga acaagagtga tacctttggt tatagatgag
      901 aaaaaattag aagatgcaaa aaaagtagtt ggtaatagaa aaatagtaaa tgtagtacct
      961 tttaaaccta agaaagtagg aattgttact acaggaaatg aagtgtttta tagcagaatt
     1021 gttgataaat ttggaccagt aatagaagaa aaagtaaaag gctttggttg tgaagttata
     1081 ggacagacta tatgcccaga tgataaagaa attataaaga gtgcaataaa agaatttata
     1141 agtcaaggag cagaacttat ctgttgtact ggtgggatga gtgtagaccc agatgatgtt
     1201 acgccaacag ctataaaaga aactggagct gatttagtga catatggttc accaatatta
     1261 ccaggagcta tgtttttact tgcttattac ggagaagttc caattatggg tataccagga
     1321 tgcgcaatgt atcacaaaac tacagtattt gatatagttt tatctagggt tttaattgat
     1381 gaaaagcttg ataagtatga tatagctaga tatggtcatg gtggcctttg tatgaactgt
     1441 gatgtatgta cttatcctgc atgtaatttt gctaaaatat aaagttatta aaaaagagta
     1501 tttacaaatt aagtactctt tttaaatatg aaacaaaaca gctagaatga tgttttaaaa
     1561 aaagcatatt tatttagaaa ttataataac ttattttcaa tgtgtaaaag aatgaataaa
     1621 gttgcaattt gatttcttta taagtataat ataagagatt gttaattttt ataaatttac
     1681 aatttttagt attattttaa taaatatttc acaaatttag tataaaaaat atactattta
     1741 cgtgataata tataataaaa atgttatcaa agttttatga taaaataaat tatgagcaaa
     1801 aagaaaggaa aatatattaa tgttacaagt tacaggtgtt ggacttagat ttggtgataa
     1861 ggaactattt aaagatgtta acttaaaatt cactaaaggt aattgctatg gaataatagg
     1921 agctaatggt gcgggaaaat ctactttttt aaaaatatta tcaggagaaa tagagcctaa
     1981 tacaggtagt atatctataa ctgataaaga aagaatgtca gttttaaaac aggaccactt
     2041 tgaatatgaa gaagaaactg ttttaaatgt tgttataaga ggtcatgaga gactttggaa
     2101 cataatgaaa gaaaaagatg cattgtatat gaaggaagac tttaatgaag aagatgggat
     2161 aaaagctgcc gaacttgaag gtgagtttgc agagcttgac ggatgggatg cagaaactaa
     2221 tgctgagaaa atacttatgg gtcttggcat aacgaaagat atgcattaca aacaaatgaa
     2281 ggaactagtt ggtggggaaa aggttaaggt attgttagca caatcacttt ttggtaagcc
     2341 tgaaatacta ttaatggacg aacctacaaa ccacttagat tttaaatcaa taaactggtt
     2401 aaataatttt ataatggatt tagaagaatc tatagttata atagtatcac atgatagaca
     2461 tttcctaaat caaatatgta ctaatatagt tgacgtagat tttggtaaaa tacaaatgta
     2521 cgttggaaac tatgatttct ggtatgagtc aagtcaattg gctttacaat tggctaagga
     2581 ccaaaataaa aagactgaag aaaagatagc tcaattaaag gaatttatag ctagatttag
     2641 ttctaatgct tcaaaggcta aacaagcaac atcaagaaag aaacagttag agaaattaga
     2701 agttgaagat atacaaccat caagaagaag atatccatat gtaggattta cacctgcaag
     2761 agaaattggg aatgaagttt tagaagtaca caatttaact aaaactatag atggggttaa
     2821 ggttcttgat aatgtgtcat ttagattgga tagagatgat aaagtagtat ttatgggaga
     2881 tgaaatagct actactgcac ttttaaacat agttatggga gaacttgaac cagacagtgg
     2941 agagtataaa tggggagtaa ctacttctca agattatctt ccaaaaaatc ataataagtt
     3001 ttttgatgga gtagaatatt ctttggttga ctggcttaga cagttctctg aagaaaaaag
     3061 tgaaagtttt ataagaggat ttttaggtag aatgttattc tcaggtgaag aagcattaaa
     3121 agaagctcaa gttatatctg gaggagaaaa ggttagatgt atgcttagta agctaatgct
     3181 ttcaaatgca aatgtacttg tattagatga ccctactaac cacttagacc tagaaagtat
     3241 aacttctgta aataaaggac ttgaaaagtt cccaggagtg cttttattta cttctcatga
     3301 ccatgaattt atatctacta tagcaaatag aattatagag ataactccaa atggaataat
     3361 ggatagaaaa atggactttg atgagtattt agaaagtaaa gagatacaag accaattagc
     3421 taaaatgtat ggaaaagata aataattatt agtataaaac cttttaatat ttgagacatg
     3481 ctatgcatga gtgtgtaaat ttttaatcaa atattaggag gttttttatg ttgtttaaac
     3541 atgtttatta gcagaataga gtaatatttt gtatattaag aatttaaata taatatagta
     3601 gatgatataa taaaaatata taacattttg gggggaatag attatggtta agaaaaggtt
     3661 agtagctgta gtattgagtt tagctatgat tagtgtaggg aatttttcat atgctcactc
     3721 aggagggcat aattcatcag gaggtcatca taataaaaat aaatcagagt taacacgtta
     3781 ttattgtgga ggacattcag cacatcaaca caataaaggt ttttgtcctt atgctagtac
     3841 aaaagaaaat tctggagtaa caatatcttc agtacataac gaaaaaaatc ataaaaacta
     3901 ttttgcagaa aaagggtata ataaaggtta tgaagatggg tgtaaagggc tttataattt
     3961 aagtgtcaca gaagccaaat ctgataattt atatgaaaaa gagattttaa aaagttcata
     4021 tgaaaaagga tatttaattg gatacaatga atataaagca aaaaaagtag atgatgtaat
     4081 gaagaagatg ttagatattc caatgttagc aagtatataa taatatagta tttaaaaaga
     4141 tattttctat aagggaatat cttttttatt gtaaagtata atttttcgta aaaaagtata
     4201 ataactgagg cgataaatta aaataaaaca gattatttaa gtagttatta atttgagata
     4261 gatacattta atattaatga atctatctca ataagctaag ctaatttaaa gctaagataa
     4321 attaatatta attagcatta aataaacttt cagaaatttt gtcagaagcc tctttatcca
     4381 tttctttaag tacatgagaa taaacgctta gggtagtact tatatttgag tgtcctactc
     4441 tctcggatat aacctttata ggtactttag aatttataag taaagtaaca tgtgagtgtc
     4501 ttaaatcatg gaatctaata tgaggtaatt cattcttttc taaaaatctt ttaaattttt
     4561 tactcataac atcttcagca ataggttcac a
//
LOCUS       DAGSHT010000245         4583 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.251, whole genome shotgun sequence.
ACCESSION   DAGSHT010000245 DAGSHT010000000
VERSION     DAGSHT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.251"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            42..1040
                     /locus_tag="MJB72_003357"
     CDS             42..1040
                     /locus_tag="MJB72_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBY8264419.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1402..1929
                     /locus_tag="MJB72_003358"
     CDS             1402..1929
                     /locus_tag="MJB72_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBY8264420.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            1956..2552
                     /locus_tag="MJB72_003359"
     CDS             1956..2552
                     /locus_tag="MJB72_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBY8264421.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2617..4200)
                     /gene="asnB"
                     /locus_tag="MJB72_003360"
     CDS             complement(2617..4200)
                     /gene="asnB"
                     /locus_tag="MJB72_003360"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBY8264422.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(4265..4402)
                     /locus_tag="MJB72_003361"
     CDS             complement(4265..4402)
                     /locus_tag="MJB72_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264423.1"
                     /translation="MKNKKFNVITLIILDNHIYAINKEQISTNSKRIYFEKSRFFSDY
                     K"
BASE COUNT         1615 a          706 c          653 g         1609 t
ORIGIN      
        1 aaattaccat aataatttaa taattacagg agggtattga tatgaaaata ctagtatttg
       61 gagcacgcga ttatgaagaa ccagtaataa aaaaatggtc tgaagaacat aaggatgttc
      121 aagtggatat ttatcctgaa aacatgactg aagaaaatgt agttaaagct aaagggtatg
      181 atggtatatc tatacaacaa actaactata tagataatcc ttatatttat gaaactttaa
      241 aagatgctgg ggttaaagtt atagcttcaa gaactgcagg ggttgacatg atacattttg
      301 atttagttaa tgaaaatgga cttatcgtta caaacgttcc ttcttattca cctaatgcaa
      361 tagctgaatt agctgttact caagctatga accttttaag aaagactcct ctagtaaaga
      421 aaaaagtctg tgaaggtgat taccgttgga tagctgaact tcttggaaca gaagttagat
      481 ctattacagt tggtgttata ggtacaggaa aaataggtgc tacttctgca aaattattca
      541 aaggcctagg agctaatgta attgcatttg accaatatcc aaatagtgat ttaaatgata
      601 tattaactta caaagattct ttagaagacc ttctaaaaga agctgacctt ataacattac
      661 atactccttt acttgaagga acaaaacata tgataaataa agatactcta gctataatga
      721 aggatggagc ttacatagta aatactggcc gtggtggttt aattaataca ggggatttaa
      781 tagaagcact agagtcagga aaaattagag ctgctgccct tgatacattt gaaactgaag
      841 gattgttctt aaacaaaaaa atgaatcctg gagaattaac tgacccagaa ataaataaac
      901 ttctttctat ggaacaagtt atattcactc atcaccttgg tttcttcact agtacagcga
      961 ttgaaaatat agtttattct agtttaagta gtgctgtaga agttataaaa acaggaactg
     1021 ctactaatag agtaaattag tattttataa atatattttt tcattaatta gctattatct
     1081 aacaaaatag cctttaattt cccaaatata agagagtcag ctcagtatat attgagtaga
     1141 ctctcttata ttatttttta tattaaactt gtactcttct tattcatacc aaattaaatt
     1201 tatattaact tattctaact actcctactc tatacatttt acctattttt acttttctaa
     1261 ttatatacat ttgaatgggt acaatctgta ttttacaaaa aaaacaccta acaaactatg
     1321 aaaattgtat gattaatatt aatatattta taagaaaaca taacaattat gttagctctg
     1381 gatttttagg gagggagtat attggaaaaa atgaataaag ttacttctaa aaaaactacc
     1441 tatattgtta cttcagcatt atttgcatcc ataatatgcc tgacaatagc atatatatta
     1501 catattccag ttggtggaaa caatggatat gttcatatag gagatgcttt tatttatcta
     1561 gctgcaacta ttttgccaac aaactatgct atagctgctt ctgcaattgg tgctggtcta
     1621 gctgatttat ctacaggtgc tgctatctgg gtaataccaa ctataatcat aaaaccaata
     1681 cttgtattat ttttcacttc taaaagtgac aagataataa ataagagaaa tatagttgct
     1741 tcggttgtag caggtatagt aggattagtt ttatacatgt ttgctgaagg tataattatt
     1801 ggaagtttta caagtgcatt tgtaatgagc ttacttggct tattacagcc aataggaagt
     1861 tttattgttt ttataatctt aggtatggca cttgataaac ttgattttaa aaaacgttat
     1921 tttaattaag aatttaaatt tatggaggga aaattatgaa tatcttatat acaattgaaa
     1981 atagtatcta tgtaaatata actaatacat gtccatgtag ttgtgttttt tgtataagaa
     2041 acgaaaaaga tgaagttgca aatagtggta gtttatggtt ggagcatgag ccaagtgtag
     2101 atgaagtaaa agaagccttc aataaataca atttagacga ctatgatgaa atcgtatttt
     2161 gtggatatgg cgaaccatta atgagaataa atgaattgat agagatagca aagtttataa
     2221 aagaaaaaag tagcataaag ataagaataa atacaaatgg tctaagtgat ttaatacata
     2281 acaaaaaaac agctattctg ttaaaaaatg ttatagatgc agtatcaata agtcttaatg
     2341 ccccaaataa agaagcttat aatagagtta ctcaaccaaa gtttggagaa aaatcatttg
     2401 attatatgtt agattttgca agagattgca aaaaatatat aaaagaagta gctttttcag
     2461 tagtagatga aatatctcca gaagaaatag aagaatctaa acaactggca aaaaaacttg
     2521 atataccatt aagagttaga cataagaatt agcaagtaaa attataaaac attaagaatc
     2581 acctgaatgg tatttcaggt gattcttcct cttgtattaa cattttacaa aatagatttc
     2641 ataccacact aggaatgtgt aaatcatcca aatctttctc caattatcag agtttccact
     2701 aatgtagtca ttaaaaatct cattaatctc atctatgtta aagaatttat ctgctatctc
     2761 actattgaat ttatctcgga tatccttatt atatcggtca tcagccatcc atatacgaat
     2821 tggcacaata aatcctaatt tatttctaaa agcaatctct tctggtaatg tttttgatgc
     2881 agctgtacga aaggcaattt tattctcttt tcctttcact ttatattctg atggtattct
     2941 ggatgcaata tcaaatattc ttgtatctgt aagaggcatt ctgacttcca atcctactgc
     3001 agtactcatt ttatctatat taaaataaat atctccttcc aaccatattt gaatatctac
     3061 atttgacatg ttagtaagtg tatctagttc ttctgtttct tcataaattc cttttaccac
     3121 ttcaatagga agcacatcag gattgtattt cttaagaatt tttcttttct caccctcatt
     3181 cataatgttt gtatttccaa tataaaatgt cttcaaattc tctccataac actctgcctt
     3241 atggtatata ttatatccac caaaaaactc atctgctcct tctccagagt aacagatatt
     3301 tgtgttttca gctgctgcct tgcatccaat agcaaaaacg atagcagatg catctccagt
     3361 tggttgctcc atatggtaca ttacataagg tactgacgca aaatactctt caggtgtaat
     3421 tatatatctc gaatttttac acccaagaat attagctgtc tgttgtgcta attttgattc
     3481 atcaaaacgc tcttcctcat aaccacatga acctgttttt tctatatctg acattgccaa
     3541 tatataagat gaatcaacac ctcctgatag gaataactct gcagtttcat tctctgattt
     3601 tacctctggc ataattttct gaagtgttgt atgaatttca tccgtccatt cctcaataga
     3661 cttgctgtgg tcaggatgaa attcaggttt ccaataacga ccaatacata ctgttttatt
     3721 ctgatagaca aggtaatgtc ctggcataag cttctttact ccacggaaga atgtattttc
     3781 acctgctaca tatgtgaaag acatataaat ctgtaacatg tcttcattta actctttcac
     3841 aaatcctggt tgctccataa tcttacgaat agatgaacca tagagaagct tcccattttc
     3901 agtctcataa taatagaaag gttttacccc aaactggtct cttaggcaga agagtttctt
     3961 ttccacagta tcccataatg caaaagcaaa cattccatgc atgtgatttg ccatatcata
     4021 tccccatgtc ttatatgctt ccactaaaat tgctcgttca cgctcatctc tacataatga
     4081 agaatcgatt ccaagttctt cacaaagaac tttccagttt cgaatatgcc cattgtattc
     4141 tagaattttt atcatatatg tgtcttttcg tgttaaatac ttttcttcta aatttatcat
     4201 atgtgtatcc tttcgtatta aaacatttaa cttctaaatt tattaaatgt ttatatatat
     4261 ttattcactt ataatcactg aaaaatcgac ttttttcaaa gtaaattctt tttgaattag
     4321 tactaatctg ctctttatta atggcataaa tatgattatc aagtattatc agtgttataa
     4381 cattgaattt tttattcttc ataaatattg ccccctttat attaccaact aaatataata
     4441 tatcttatgt tacttacata ttctttaaga aattctgaag attttataaa gttttttgta
     4501 ttataattaa ttaaaaataa aattctagtt ataaaacata ttttccatta aacaaaaaaa
     4561 tagttaaaat aaattcataa cca
//
LOCUS       DAGSHT010000246         4531 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.252, whole genome shotgun sequence.
ACCESSION   DAGSHT010000246 DAGSHT010000000
VERSION     DAGSHT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.252"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            23..253
                     /locus_tag="MJB72_003362"
     CDS             23..253
                     /locus_tag="MJB72_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBY8264424.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            266..415
                     /locus_tag="MJB72_003363"
     CDS             266..415
                     /locus_tag="MJB72_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBY8264425.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            672..1109
                     /locus_tag="MJB72_003364"
     CDS             672..1109
                     /locus_tag="MJB72_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBY8264426.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1366..2259
                     /locus_tag="MJB72_003365"
     CDS             1366..2259
                     /locus_tag="MJB72_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY8264427.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            2437..3261
                     /locus_tag="MJB72_003366"
     CDS             2437..3261
                     /locus_tag="MJB72_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8264428.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANILHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3360..3968
                     /locus_tag="MJB72_003367"
     CDS             3360..3968
                     /locus_tag="MJB72_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY8264429.1"
                     /translation="MGKFLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            4072..4248
                     /locus_tag="MJB72_003368"
     CDS             4072..4248
                     /locus_tag="MJB72_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264430.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         1725 a          518 c          625 g         1663 t
ORIGIN      
        1 ttattatatc ggaggtaatt atatggaaat taagaaaaaa ttagtcatga gctttaaaaa
       61 tgtaaaggat aaacaagttt ctttttctat agaaaatcca aaggatgatt taactgaaga
      121 aaatataaaa tctgtcatgg atttgatagt atctaaaaat attttttcgg ttggaggatt
      181 tgacctagct tctgttgttg aagccaaggt tgtggaaaca aataccactc cttatgactt
      241 agttatagaa tagggggcta attttatgga tgatagttta caactgttaa taaccaatgt
      301 tggttttcct atagctctat ccatgtactt attggttaga attgagggta aattaaatac
      361 tttatcaaac agtattgata cattatccaa aaatattttg atatttaaga agtaatttga
      421 caatatatgt aagtcataaa ataggtctat ttgtaaaata attagacata ttctatgtga
      481 aatactagga tatttggata atttatccaa atatccatag tatttttttg aattttacca
      541 cctcaaattt gaatttcact gttttatttt tgaatttcac tatgcttaat ttgaaattta
      601 gaacgcatct agttggactc aataagatat aatgctattc taatgacagt aataaaatga
      661 ggtgatgatt aatgaaaata catgtcgaac aaggtattaa ttgtgatgat attgaaataa
      721 ccataaaatg ttctgctcta gataataaca ttgaaaaaat aatttcatta ttgagtacac
      781 cgttacttga tataaaaggt aaaaaatcag gagagattta taaactcaat gtctgtgata
      841 ttttatactt tgagacagtt ggaaataaaa catttgctta ttatgaagat gatgtatttg
      901 aaattgattt aaaaatatat aaattatcaa aattattagc taatacatcc ttctcacgaa
      961 tatctaaatc catgatttta aatatggact acataaaaag tataaaagta atatcacata
     1021 atcgcatgca agctacttta aaaaacaacg aaaaagttat tataaatcgt cgttatataa
     1081 aaaatattaa agaaaagcta aacatttaat aaaaatctaa agtaatttat taatatcact
     1141 aattgcaaat ttattgacct atttaataat taataataat ataatatatt caatattaaa
     1201 tactaattaa ttatatttag tgtaaaaata attaatcaaa atataaaaaa taggatatta
     1261 ttataggcaa taactaacat caagcgataa cttaatacat ataaaaataa ataaacaatt
     1321 aataaaaatt aaaacttcat tataaaacgg aaggatagtg aaaaaatgca actatataac
     1381 acccccttta atacaaacaa acacttaatc tatttcttta catttacgct agtattgaac
     1441 tggatattca gtcttacacc tgcgttgctc ggaattacaa atacaatact tggagaaatc
     1501 attttttatc ttgcatatac atcaccagct attgtaggtc tattttttat ttctacaatc
     1561 tatccacaaa gtgcaaaaca agattttatt tatagatacc taagctttaa agaaattaga
     1621 ataaaatgga tactattaac aatattattt tttgctttaa cttttattac ttcgttaata
     1681 atcggaaatt actttaatgt aaaaacttct gatatagatt ggatacacat agcagtgttt
     1741 agaccttcta aaatagctta tatgctatta gcaagtttta tattgagtat acttagtcaa
     1801 gaagctggat ggagaggata cgccattgac aaattattag ttagatttgg atttataggc
     1861 tcttcaatta ttttagggct tgtttgtgga atctggtatc tagggtcata ttttacacct
     1921 aatcaaatac catataattt ggcacaatac tccctatttg atgctttttt atttatacca
     1981 aacattgtac ttttaaactt tataattaat tttgtatata tcaatacaag tagaagcatt
     2041 ttatcagctg gattagttca tatgatgtac tattttttca acattcaatt acttctacac
     2101 taccctatta aattatgtgt aatagctcaa tatgtacaaa taatatttgg tttaatattt
     2161 cttgtatata tgatttcttc taataaattt aaacagaagg taaattctga aattgaacag
     2221 ataaagtcgg atgattttga atttgagtta gactggtaat tttaatatga attataaatt
     2281 aatataatct atggtttgat acgattacta cttaaatata atttactctc tataatttat
     2341 tgcaacatct ggaaaataat tgtatttcca caaacgcttg aagtatataa aaacaaaata
     2401 tatactgatt ataagtgaaa aggaggtcat attttcatgt ttaaaatagg agaggtttca
     2461 aaactaacac aaatttctat tcgtatgttg cgttattatg atgaacttgg tattttaaaa
     2521 cctgcagaaa tagacaaata tacaggtcat agattatact cagttgaaca gatttcaata
     2581 ttacaaagaa ttgtattatt gagagattca aaatttagcg ttgcagaaat agcaaatatc
     2641 ctacataatt ggaatgatga atttgtaatt aaagaattga atagaaaaaa gaatgaaata
     2701 caaaaggaaa taaatcagga gcaacaaaga ataaataaaa ttgataaatt tatagaagct
     2761 ataaactgtg ataaagatga aattcattac aatgttgtct ttaaaaagat tccaagttat
     2821 aaaatcatat cacttagaga gataattcca gactaccaat ctgaaggtat actctgggaa
     2881 aagctatcta aatttatcaa agaagaacat atagaagtat cacgtcaatc caacaataac
     2941 atagcttttt accatgatga agaagtcaaa gataatggtg tagatattga agttggaatg
     3001 gttgtaaaga agattggaaa gaacaaatct ggatttatat atcgtgagac agaagaaatt
     3061 gatatgatgg catgtactat ggtttatggt ccatatgaaa atatagctgg tgcatatgaa
     3121 tcgttctgtt attggttaga taaaaatagt gattatcaaa tatcaggtat aaatagacaa
     3181 atcggtcata aaggtgaaca taatgaaata aatcctgaaa attatcttac agaaattcaa
     3241 atccccctta taaaagtcta atcgattagt acttaaatac ttaagtacta atcacccatt
     3301 gacatcccaa aattttcatg atagtatcta aacgtgtatt aaacatgttt aattttttta
     3361 tggggaaatt tttaagggga aaaattatga aatcagattc aacaaaggaa caattaatta
     3421 atgctacaat ccagttacta tcttcacata gagatgtttc aagcattacg gctagagaaa
     3481 ttgtagctaa agcaaatgtc aatttagcta tgattaatta ttattttaaa tctaaagagg
     3541 agttaattaa tatttctatt aaaaaatttt taaagcgtca tatggatgaa tataaggcta
     3601 cacatgatac taaaaattta gcacctaaac agcaattaag ggaaatacta attgcatttt
     3661 ttgattttat aggtgaacat agtcaattta taaagatttc tgtaccctat atattgatac
     3721 aggaagaaat tgtttatcca tttgagatac tacctttaat taaggctcac tatgaaaatc
     3781 agaataacaa aaaaactgaa gctgaataca gaactattgc gtatcaaata acttcattta
     3841 ttcagttgct ttttcttcgt tctaatgctt ttttcaagta tagtggtatt gatattctta
     3901 atgttgaaca gagaaatggt ttaatagatt ttcaattaga tttattattg gtagatatta
     3961 caaaataata ctatattaat tttttatgta cattttttta ttttatggta acgtttgaaa
     4021 atattctcat tgactaaaaa tacttgcatt aaataagggg gaggaatttt aatgtttttt
     4081 acatttatag gagtaatatc agcaatttta ttgttgtgta caattatatg tggtttatgg
     4141 ataaaactta ataatcgtaa taatgactct aattctcact ttactttttc aatactttca
     4201 attgcttttt cactgttaat tgtggtggcc tttttagtaa gaaattaatg tctttgttat
     4261 gctggggtct attattatag atttttatta ttgcatgatt tttactatat tatagtattt
     4321 ttattatatt acagggtctt atttattata gtgttttgtt gttacagcat tttattatat
     4381 tacagagtcc tatttattat aggattttta ttatattgta aaatcttatt tattatagga
     4441 ttttgttata ttacaatctc ttatttattg cagtgtttct attatattgc aaactcttat
     4501 ttattatggt gttttgttat tgcaacattt c
//
LOCUS       DAGSHT010000247         4504 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.253, whole genome shotgun sequence.
ACCESSION   DAGSHT010000247 DAGSHT010000000
VERSION     DAGSHT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.253"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            99..779
                     /gene="pgmB"
                     /locus_tag="MJB72_003369"
     CDS             99..779
                     /gene="pgmB"
                     /locus_tag="MJB72_003369"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBY8264431.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            776..3061
                     /locus_tag="MJB72_003370"
     CDS             776..3061
                     /locus_tag="MJB72_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBY8264432.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            3286..4179
                     /locus_tag="MJB72_003371"
     CDS             3286..4179
                     /locus_tag="MJB72_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBY8264433.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         1872 a          409 c          781 g         1442 t
ORIGIN      
        1 agttagagta gataatttta taaataatat cttaaataca agatagaaaa taaattgatt
       61 attagtctaa aaatttcttg tagaaagagg aaataagaat gattgaagca tttatatttg
      121 atttagatgg tgtaattaca gatactgcat attatcacta tatggcttgg agaaaactag
      181 cccataaagt tgggatagat atagacacaa aatttaatga atctttaaaa ggcataagta
      241 gaatggagtc attggacagg atattagaat ttggaaataa gaaatattca ttttcagaag
      301 aagaaaaagt gagaatggct gaagaaaaaa ataattacta tgtatcattg atagatgaga
      361 taacttcaaa tgatattttg ccaggtatag aaagcttgtt aattgatgta aaatctaata
      421 atataaagat aggtctatct tctgcaagta agaatgcaat aaatgtatta aatcatttgg
      481 gtataagtga taaatttgat tttatagctg atgcaggaaa atgtaaaaac aataaaccac
      541 atccagagat atttcttatg tcagcgaaag ggcttaatgt aaatccacaa aattgcatag
      601 ggatagagga tgcaagtgca ggtatagacg ctataaactc agctaatatg ttttctgttg
      661 gtgttggaaa ttatgaaaac ttaaaaaaag caaatttagt tgtagattca acaaatcaat
      721 taaagtttga atatatacaa gaaaagtata atgagtatat agttaggaga attatatgaa
      781 atgtttattt agtaatgtag atgagtggaa gattatacaa gataagattg aaatcaaaga
      841 aaatagatta gcagagtcca taatgagtat aggcaatgga tatatgggta tgagaggaaa
      901 ctatgaggaa aattatagtg gagatacaca caaggggtct tatatagctg gagtatggtt
      961 tccagataaa actcgtgttg gttggtggaa aaatgggtat ccagaatact ttggtaaaat
     1021 tcctaattca gtcaactata taggtgtaag aattttcata gatgatgtag aattagattt
     1081 agcaaaatgt aaagtagaag atttttatag agaattagat atgaaaaatg gtattttaaa
     1141 tcgtaggttt attgtaaata taaatgataa aaaatttgaa gttagggttg ctaggttttt
     1201 aagtatatct gttaaggaac ttgcagtaat taaatacagt ataaaatcat taaattctaa
     1261 tactaaaata aaatttgtac catatttaga ttcaaatata aaaaatgaag attcaaacta
     1321 tgaggaaaag ttttggaatg gaatttcttc aagttcttta gaatataaag ggagtattct
     1381 ttctcgaaca aaggagaatg catttaatac accagtattc actgtgggtt caatgatgta
     1441 tattaagaca aatggtgatg tagaagctat atctcatgag agtgattata attactgtga
     1501 aaattcgata acaatggatg ttttaaaaaa tgaggttgca agtatagaaa aatatgtatc
     1561 tataacttca actagagatt ataaggaaag tgagctatta agaaaatgtg aagacatttt
     1621 aaatagagaa ctatctaaaa gttatgatga tatattaaat gaacatacaa agttatggaa
     1681 taaaagatgg gaaagtgcag atgtaaaaat aaatggagat aaaagtagcc aacaaggtat
     1741 aagatataat ttattccaac tattttcaac atattatgga gaagattcgc gattaaatat
     1801 aggtccaaaa ggatttactg gtgaaaagta tggaggagct acttattggg atacagaagc
     1861 attttgtgtt ccagtatatc taggtgtagc agataaggag gtaactagaa atcttttaga
     1921 atataggtac aatcagttag aacaggcaaa ggataatgca aaaaaactag ggcttaaagg
     1981 tgctttatat ccaatggtaa catttgatgg agtagaatgt cataatgaat gggagataac
     2041 atttgaggaa atccaccgaa atggaagtat agtatatgca atatataact atacaaatta
     2101 tacaggtgac tataaatata taaaagaaaa aggaatagat gtaataatag aaatagctag
     2161 attttgggca agtagagttc atttgtctac aaaaaaagat ttgtatatga tacatggagt
     2221 aacaggtcca aacgagtatg aaaacaatat caataataat tggtatacta attatatagc
     2281 taaatggtgt ttagattatg ctgtagaaaa tatcttacga ttagagaaag agtgtaatga
     2341 atgtattgac agaaataatg tgactaggga agaagttgag ttttggaaaa taatatcaaa
     2401 taaaatgtat ctgccgtatg atgaggaact acaaataata attcaaaatg ataatttttt
     2461 agataaagaa tttgtagaag taaaggattt accaaaagaa aattttcctc ttaatcaaaa
     2521 ttggtcttgg gataagattc ttaggtcgtg ttttataaaa caagcagatg tattacaggg
     2581 aatttactat tttggaaata gatttactaa agaagaaaaa aagagaaatt ttgacttcta
     2641 tgaaaaatat actgtacatg aatcttcttt atcttctagt atttattcta ttattgcctc
     2701 agaaatagat aacctagaaa aggcttatga attatattca agaacagcta ggcttgattt
     2761 agataattac aataatgata caaatgatgg actgcacata acttcaatgt ctggttcatg
     2821 gctttccatt gtacatggat ttgcaggcat gagaacatgg aatgaaactc tatcttttga
     2881 ccctaagttg ccaaaagagt ggaaaaatta ttctttcaat ataaattata gagataatat
     2941 tattaatgta gatgtatgta gtgatttaat taaaatagaa aatttaaaag gtagaccaat
     3001 aaaaattatt gtatatggga aaaaatatat attagaaaaa tgtataaaaa ttgataaata
     3061 gatttaataa tatttttatt atataaatag ttaaaataag ctatctaatt atttatttat
     3121 tttaactatt ttgtgtgata gaaaactata tattataaca atagtagtgc ttttaattaa
     3181 atatatttca taaatcttca taaaatcttc aaaatttcgt aaagaatatg tcacaaaagt
     3241 aaggtatatt atattcagca gatagtataa gggaggacaa tatttatgaa gaataaaaaa
     3301 ttcattgtta taatactgat aatatcaatt ttaataggtg tattagtgaa ggaatattca
     3361 tctagagaga taaaaaagga tgatataaat gtcagtaagt atataaaata tgctgattta
     3421 gcaagtaaaa ataatgcaca agttaactgg aagtatgtag cttccattgt tgctgtgtta
     3481 aataaaaata acttaaaaaa cgttaaggat tcacagatac aagaagtatc agatttattt
     3541 gttaagaatt tttctaaaaa taataaaata aataagttaa gtgatatttt ggatgagtta
     3601 gaatttagta atagacaaaa aagattggtt gataattata tagacaatct taaggattat
     3661 ggtataaaac cagaaagatt gaagtctgat acaaaatata tgaagtttat tgatgaaatt
     3721 aaaacagaag caatacaaaa ttataaggat tataaaatac ttccatctat aactattgca
     3781 caagctataa tagaatctag ctggggcaag tcaactttgg caaaacaata tagcaatcta
     3841 tttggtataa aggctgacgc ttattggaaa ggtaagtcag tgactttaga aacgaaagaa
     3901 catttagata caattataga tgataaattt agaatatatg atgataaaaa tgaatctata
     3961 aaagaccatg caaaattttt agccacaaat aaaaggtata aaaacaatgg tgtatttgat
     4021 gcaaaaacgt atatatatca agctaaagca ttagaaaagg caggttatag tactgcgaag
     4081 gatgaaaatg gcaatagtat atatgcagct cgtctaatag aattaattca acagtataat
     4141 cttcaattaa tagacagtga aattcaaagt gaaatgtaac tttgattaaa tttttattga
     4201 taaggctatg atttttaaaa tcatagcctt attttgacat gcttttactt gtaattatta
     4261 aatcgatatc attatctttc aaaatttact ttatactgta aatcaaatta tttaagttaa
     4321 aacttggaat tatttataaa aggattattt actttatcta aattctgcac ttaaagaaca
     4381 tcttttccct taagactgac cgttagtcta taaaaattat aattacttat aaaaaagtga
     4441 atatatatat aaagttttca gaaagacttt tacttgtaaa ttgaaatcaa tgaagtaaaa
     4501 aagg
//
LOCUS       DAGSHT010000248         4460 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.254, whole genome shotgun sequence.
ACCESSION   DAGSHT010000248 DAGSHT010000000
VERSION     DAGSHT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.254"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1110
                     /locus_tag="MJB72_003372"
     CDS             <1..1110
                     /locus_tag="MJB72_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBY8264434.1"
                     /translation="VNRFLPTVVNYGTNFESLYSIFLIAINVLKDENIECNKIEFGKE
                     INDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVNLSFDDLLPYLNRDIRDFEVY
                     WHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKDKLTQEYYDDMLFFIVLKGSK
                     SLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSLEELKIVKEAYEKEKNVILKD
                     ELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAVAGSRFRNREYLEKELEKSKI
                     YYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILSNLLDGGKLLYCRVTEYNLYE
                     DCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(1288..2652)
                     /locus_tag="MJB72_003373"
     CDS             complement(1288..2652)
                     /locus_tag="MJB72_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY8264435.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLELFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(2831..4174)
                     /locus_tag="MJB72_003374"
     CDS             complement(2831..4174)
                     /locus_tag="MJB72_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBY8264436.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(4247..4348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1642 a          635 c          640 g         1543 t
ORIGIN      
        1 gtgaatcgat ttttacctac tgtagtaaat tatgggacta actttgaaag tttatattca
       61 attttcctaa tagctataaa cgtcttaaaa gatgaaaata tagagtgcaa taagattgaa
      121 tttggaaaag aaataaatga tatcttgctc agtgaaaaat ggaaaaatat atattttgaa
      181 gcattaagag atgctagtgg aaaaacagaa gatattataa aaatgtcaga aatttatgat
      241 gtaaacttat cttttgatga cttgttgcca tatctaaata gagatattag agattttgag
      301 gtatattggc atatatctaa aaagggaacg acaagttcta gattaaaatt attaaatttt
      361 tttgaagaga cgtttaaaat agatgattta attggaaaaa tgaaagatat agaaaaagat
      421 aaattgactc aagaatatta tgatgatatg ttatttttta ttgtgcttaa aggaagtaaa
      481 tcactatatc ctgaaggaaa gaatatatct ttaaaaggaa tatttggaaa tataaatgaa
      541 gtaagaaaag aatccataaa tatcttaaaa agatacagag agaaactatc tcttgaagaa
      601 ttaaaaattg ttaaagaagc gtatgaaaaa gaaaaaaatg ttatattgaa ggatgaatta
      661 agaagagtat tatatgaaag taataattta aaaaaagaat ttgtaaatat tgaaaaaata
      721 aaagtagatg aacatggtaa ggatatctat ttgacttcaa tagctgttgc tggttcaagg
      781 tttagaaata gagaatatct tgaaaaagaa ttggagaaaa gtaagatata ctatttaact
      841 agagagaaag ataatctata tgatgaaaaa gctattaaaa tagttggtga aactggatat
      901 gtgataggct atgtcccaag aaaagaaaac tatattttga gcaatttatt agatggagga
      961 aaactgctgt attgtagagt aactgaatat aatttatatg aagattgtat ctacgcaaat
     1021 gtgtacttga gttataaaga tgtaattgaa acagttgaaa actctttaaa gatggtttta
     1081 gataagagta gaataaaact tataaactaa gacgtttata tattgcttag atgatgtaat
     1141 cctaactgat tggttatttt ttctggaatt atggtgtatt ttatgagcaa tgctaatgaa
     1201 tataaaataa aaaaacaatt gttttgtata tatgatgtat ataaatatat ttatatattc
     1261 atatttacaa aacaattgaa atttatgtta ttttataatt ttaaatttag atggaatttt
     1321 aacttctgct ttatctttta ttttagaagc ttgtttttta actttattta ataattcaga
     1381 ttcatctaca actaatactt ttctttcctt catcaaccat ttaccatcac aaatagtaga
     1441 gtctacattt tcactagtca tagaataaac tatatttcca attggattat gcattggtaa
     1501 acaatgaatt gtatctggat ttagaataat catatcagct ttttttccta cttcaagact
     1561 accaatttct ttttctagta aagcacattt tgctccattt atagtagcca tttctaagac
     1621 ttcttcagct ggtacgacag ttgggtttaa agttcttcct ttatgtatta gtgatgtaag
     1681 atacatgtcc ctcataaggt ccattctatt atttgaagga gctccatcag taccaattga
     1741 aacaggtatt cctttttcta acatttcagg aattgatgca aaccctagaa ccactttcat
     1801 agctgaacct gggtcatgag aaacttttac atcatgtaat ctaaataaat caatttcctt
     1861 atgagtaagc caaactgtat gagcagctaa aagatttggt cctaatacac ctagtttatc
     1921 aaggtattca actgttccaa ctccattgtt ttgctttaca aatgaaattt catcagctat
     1981 ttcagcaata tgcatatgta tacctgtatt gtatttatca gctagttctt tagttctaac
     2041 aagtaattct tcagttgtat taaaaatagt tcttaatgca aaccatattt taattctctc
     2101 attagatgta ttattaaact tatggaataa ttctaattga gtattgattt cctcatcagt
     2161 agttttttgc catgcctcag gaagaccatc tcctgtatcc ataactgatt tagctaaaca
     2221 ggctctaagt ccagttttat caacagcttc aaccatagca tctacatatt gaccaccaga
     2281 ttctaaaaat gtagtgacac cagattttat caattcaaca caacatgcag tagatgaaat
     2341 caaggaatct tcataattga agctactttc gtatggccaa attctctctc taagccaagt
     2401 taaaagtaca acatcatctg caagaccacg tcctaattgt tgagataagt gaacatgcgt
     2461 attgactagc cctggcatta gtatttttcc tttagcatca tatatctctg catctttatc
     2521 tacaatatta atatcaaact taccaacctc ttttataata ttattttcaa taagtatgct
     2581 tccattttca tatacctctc tatctgcatt catagtaatt agatatgcat tttttataag
     2641 taattgtgac ataaacacca ctccttttct atatattttt atatctatat attatgataa
     2701 taaattttct atacaaagtt aatagcaaaa tcaatttaca tataaagtga ttttgctatt
     2761 tataaattta aaataaataa gattttattc tatataattt aaaatattga cctagaacta
     2821 tttaaaaata ttaacttaga actaagttta caactgcaag taaacctata atccaaagtg
     2881 taatactgat tttatcagtt tctttagcta ctacatgcat tattatatat gaaataaatc
     2941 caaagcataa accaatactt atactataag ttaaaggcat caataaaact gtaacaaaag
     3001 caggtacagc tattttgcta tcagcaaaat caatctgttt tatgctttca aacatgaata
     3061 caccaaccat tacaagtgca ggagctgcag cgtaagtagg aactatacca agtaatggtg
     3121 taaatataag tgtagctaag aataatgctg ctgttactat tgaagcaagt cctgttcttg
     3181 cacctgctgc aataccagaa gcagattctc caaatgttgt aacagtacta gttcctaaga
     3241 atgagcctat taaagtagaa gaaacatctg catataacat tcttcctaaa tcttttattt
     3301 caccattctc atcttcaaga cccatttgtt tacaacaagt aataagaaat gctaatgaat
     3361 caaacatgtc tataaacatg aaagagaata taggtccaat taatgagaat ttcaatgcac
     3421 ctacaatatc taatttaaaa gcaactggtg ctatactagg aggtgttgag attattgctg
     3481 ttggcaactc cacaaatcca aatataacag atattattgt tgcaactact atacttatca
     3541 atattccacc tttaactttt ttatattcta gtattgccat taagataaga gcaaaaagag
     3601 ctattaaaac tgttggtgta aaatctgcaa gtgaaactat agtatctgga ttagctatta
     3661 taacacccat attttttaaa ccaacaaaag caaggaaaag acctatacct gctgaagatg
     3721 ctactgccaa ttctctagga attgcgtctg caattgtctt tcgaagacct gttactgcga
     3781 gtgtaaagaa gacacatcca gatagaaaaa ctaccccaag acctgtttgc catgggattc
     3841 ctgcacctat aaccaaagag tatgtgaaaa atgcatttaa tcccatacca ggagctagag
     3901 caaatggtgc atttcctaaa atcgcaaata taagtgtacc aatggctgta gctagtatgg
     3961 taactgttat aagggcggct ttatccattc cagcttcaga taatattgct ggatttacga
     4021 aaactatgta tgccattgtt aagaatgtag taactccacc tattacttct gttttaaggc
     4081 tagagccacg ttcgctaaat ttaaacattt tgtctaaaaa gcttaatgat gaattatttc
     4141 cacctggttg tacattgtta gtgtttggta ccatttttac ctccaaaaaa ctttaagata
     4201 taaacaaaaa aatatatatt catatagata gaatagaaca tatattcaga gttgacattc
     4261 tattcataga aaacccattt aaggtgagtt tgtagaaact attagaccgt attactaata
     4321 ttatatgaac attttctatt tgtttttctg ttgtattata tcgtaatatt gtattattta
     4381 caataaatat aataaaatta ttcgtttttt tgtcgatttt ataaaaaatt aaggttattt
     4441 attgaaaaaa attacttttt
//
LOCUS       DAGSHT010000249         4428 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.255, whole genome shotgun sequence.
ACCESSION   DAGSHT010000249 DAGSHT010000000
VERSION     DAGSHT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.255"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(140..748)
                     /locus_tag="MJB72_003375"
     CDS             complement(140..748)
                     /locus_tag="MJB72_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBY8264437.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(1008..1553)
                     /locus_tag="MJB72_003376"
     CDS             complement(1008..1553)
                     /locus_tag="MJB72_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBY8264438.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(1671..3494)
                     /locus_tag="MJB72_003377"
     CDS             complement(1671..3494)
                     /locus_tag="MJB72_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264439.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(3507..>4428)
                     /locus_tag="MJB72_003378"
     CDS             complement(3507..>4428)
                     /locus_tag="MJB72_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264440.1"
                     /translation="LIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEK
                     DLKFVNCDIIFENVDFSYNKNTDEKILKNISFKIEEGQNIGVIGQTGSGKTSLVNLIQ
                     KLYIIDKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKED
                     VVKSCEIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDC
                     FSAMDINTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVV
                     GFATHEDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1476 a          802 c          381 g         1769 t
ORIGIN      
        1 gcacaactaa cactataatt ctagtgatta attttatttc aaaaaatata ataacactac
       61 taatatttta aatatcaata gtgtcataaa aacttttcag attaattaaa tctttctata
      121 tattttataa taatctaaat catgctaaat atttagacag aaaacttttt tctttaatag
      181 taaatacttt tgtttgaata tcagaataat tatttgaata tctatctata aaaccatgat
      241 ttgcatctaa aatctcaatt tggatatttt gtttcctaga aagttggtaa gaaatagaat
      301 caacatcaaa tgaatcgtgc tttgcaaata ctaataaggt aggacatttt ggaattacat
      361 ccatgtaatc tctaatccga gagccataac aacaaattat gccatcacaa aaagaatctt
      421 cactgcatct ccatgcaata gttgctccca cgctataccc agcaacaaat acatatttat
      481 actctgattt taaatcattt ataagctttt caactttttt ataaatatca aaaccaacat
      541 tatttataaa aaaatcatat gcctcttgga cttttgaata tggaaaaact cttttttctc
      601 ccagaagttc aggacaaaat acatcatatc catatttata atacttttga caaacatctt
      661 ctataaagct attaatccca tagatttcat gcaaaacaac aatagctaca tcacttttat
      721 gtatatactt tatcagatta tccaccacct ttacaaaaca gcaaattttt tatacttagt
      781 ttaatatatt cttctttaca tgtcaattta cttttctaaa atcaaattta tttttggatt
      841 aatcatttat ttttaaaagt tgtgcattat tactattaaa ttattctcta ttattaaaaa
      901 tataatcgtt agaaataaaa aataaagtac aaataattaa aaaatatctc actcataact
      961 ttaaaaatta taagtgagat actcctaaaa tctactttat attaaattta ttttccaaaa
     1021 tatctattta ataatcctaa aaatgcttta ccatgtctag cttcatcttt acacatttca
     1081 tgtactgtat catgtatagc atctaatcct aattcttttg ctttcttagc taattttaat
     1141 tttccatcag ttgcaccata ctcagcatca actctaactc ttaagttttc ttttgtatct
     1201 gcaactacaa cttctccaag aagttctgca aatttagcag catgttcagc ttcttcaaga
     1261 gctattctct tataagcttc tcctacttct ggataacctt ctctatcagc ttgtctactc
     1321 attgctaaat acattcctac ttctgtacac tcaccaacaa agttagctct taatccttcg
     1381 attatttctt catctacacc ttgagctact cctattctgt gctcatcagc ccaatttttt
     1441 tcgcctttca tttcttcaaa tttatcagct ccaactttac atactggaca ttgtgctggt
     1501 gcagcatctc cttcatgtat atatccacat actgtacaaa caaatttctt cataattact
     1561 ttcctcctaa attttatata tcaaattaaa ttttttattt attaagttag tctatatata
     1621 cccgcaccat atacctttaa acaatttttt taaaagtttt ttctaaatac ttattttccc
     1681 tgtgtcaata atatgtttct ataataatgt ccctttaatt ttatcaattc tttatgattt
     1741 cctgattcca caattctacc attttctaaa actattatat tatcacaatt tgatatggta
     1801 cttaatctat gagctataat tatacttgtc tttccataag ttaaattatt tattgcttct
     1861 tgaattttag cttctgttgc tatatctatg ttacttgtag cctcatccaa aataagtatt
     1921 ggagcatttt ttaatactgc tctagcaata cttattaatt gtttttgacc ttcacttaaa
     1981 tcttcacccc tatccgataa tacagtacta tatccttcac gcattttaac tataaattta
     2041 tgtgcatttg ctattttact agcatatata acttcttcat cagtagcctc cagatttcca
     2101 taccttatat tttccataac tgtatcattg aatatataac cttcttgtgg aactactcca
     2161 aaacaagttc tcaaatagtt tctgttaata gagtatatat ccttaccatc taaaaatata
     2221 cttccttcat ctatatgata aaaatttgta attagattta taattgttgt ttttccactt
     2281 cccgtctctc ctatgatggc atttgatgtg ccagctttga tttttaggtt tatattatct
     2341 aaaattttct tttttgaatc ataagaaaaa cttacatctt taagctcaat ttctcccttt
     2401 acaaaatgag agtttaaatt accatcttta ttatcgttaa tctcactttt aaacatgtta
     2461 tgcacttctt gctcttcttg ttcatctata atttcaaaaa ctctttcaca tccagctatg
     2521 gctgaaagta atgtattaaa tagatttgct aattcattta aaggtcttgt aaattgtctt
     2581 acataactta aaaaactagc tataacccct atggttataa tatctttaga tgcaagaatg
     2641 cctcctataa tacatatagt tgcaaatcct atattactaa tcacattcat aataggcatt
     2701 aaaagagaag tgtaaatttg cgatttaagg gaaatattta gaagtctatc attgacttct
     2761 ttaaaatcat ttacagattt tctttcataa ttaaaagatt ttactacatt tatgtttgat
     2821 atcatttctt caatatgtgc atttagtttt cccaactcaa cttgttgttg cttaaaaaaa
     2881 actctagttc tctttgcaat aaaattactc acaaacatta ctatcggtat taatattaca
     2941 ctgcaaaagg ttaaaattgg gcttaataca aacatcataa taaaagtcat caatatgttt
     3001 aagattcctg taattaactg tactaatgaa tttgatattc ctgtactgat attatcaaca
     3061 tcatttgtca ctctactcat aatatctcca gtagaacttt tatcaaaaaa ggttattggt
     3121 agatttttaa attttaaaaa taaattactt ctcatttctt taactatact ttgagataat
     3181 ctagctgtac atatcccctt agacagttta actgaaaact caattatata aaatgaaagt
     3241 aaaataatta ttccttttaa gaaaatggat tttgaataag tatcgatatc tattatgtca
     3301 actacctttc caatagtata tggtattaca gttgatataa ttgaatcaat tattactagc
     3361 aaaaaaataa atctagtaat cttcttatct tttttaaagt acataaaaat tcttttaaac
     3421 gtttttttaa aatctttagg cttttctttt ttctttaatc tactgacctt ctgatttcca
     3481 gtacttaaat tcaatatact tcctccttaa tttacatctt cacttactaa attttgcaac
     3541 gcaaaaattt ttttataaat tggagaacta accagtaaat cttcatgtgt agcaaatcca
     3601 accacctcac cctcatccaa gactaaaatc ttatcacagt caattataga actaatcttt
     3661 tgtgaaatag ttattacagt acatttctta ctttcctttt ctcttaaatt acttatatac
     3721 tcttttatat tctttcttat aattgtctct gtattaatat ccattgcact aaaacaatca
     3781 tcaagaatta atatttgtgg ttttttggct atagctctag ctatagtaat tctttgtctc
     3841 tgtcctcctg ataaattttt acccctttgg aataaaactt catcataagt attttcgagt
     3901 tttgttataa attgatttgc atttgctatt tcacatgatt ttacaacatc ttctttgcat
     3961 atatctctat ttcccataac tacattctcc aatatacttt ttccaaacac actactattt
     4021 tgaaatacta cacctattct tgcccttaaa ctttcttcag aaatactgta tatactacgt
     4081 cctccaatct taatatcacc tttatcaatt atatataatt tttgaattaa atttactaaa
     4141 gaagtctttc ctgaaccagt ttgaccaatc acaccaatat tttgaccttc ttctatttta
     4201 aaggatatgt tttttaatat tttttcatct gtatttttgt tatacgaaaa atctacattt
     4261 tcaaaaatta tatcacagtt tacaaacttt aaatcttttt ctccaacatc taaactttct
     4321 tctctctcaa ttacttcttc attctcatca accaaaacct cacttattct ctcataagat
     4381 gcttttgccc taacaaaaac attaaaaaca tgtgaaatca ttataaga
//
LOCUS       DAGSHT010000250         4424 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.256, whole genome shotgun sequence.
ACCESSION   DAGSHT010000250 DAGSHT010000000
VERSION     DAGSHT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.256"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1559
                     /locus_tag="MJB72_003379"
     CDS             <1..1559
                     /locus_tag="MJB72_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264441.1"
                     /translation="MTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLIIFIVIGIRNTI
                     VIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFIAENIMLSAFIL
                     VTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKSNSSVLAVIAIL
                     IATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLLYKYKKDNKIIF
                     DKTIELIDKDINIEMYYKNGNSYIEDRMSVDIIRESDFKKLRQYQNNNYEELTSKESV
                     YYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEINSQATFDLIVVK
                     DDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYPFNFTSSIESYN
                     NLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIKLGANNKDIEK
                     IISKQLKVIFLMPLVVGTVHNLFAMSIVQKFIPRSLLVPIIITLVIYFIGYFIYYFLT
                     LKYALNMIKE"
     gene            1784..3397
                     /gene="ggt"
                     /locus_tag="MJB72_003380"
     CDS             1784..3397
                     /gene="ggt"
                     /locus_tag="MJB72_003380"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBY8264442.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGSVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(3530..4381)
                     /locus_tag="MJB72_003381"
     CDS             complement(3530..4381)
                     /locus_tag="MJB72_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBY8264443.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTIIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1720 a          523 c          692 g         1489 t
ORIGIN      
        1 ttatgactat gatgcttgta aaattaatga atgaaatgat agtagtaaat atgaacttaa
       61 gtataaaggc cttagttcag acactagcag tatttttaat aatatttata gttattggaa
      121 taagaaatac tatagttatc agaaataaga gaattataga gttatttaat aaatatccag
      181 aaaagtataa gtttaaaaaa tttataaagt taaaatgtgt acttggtgtt ttattaatag
      241 ctatttctta tttaatgtca attagttatt ttattgctga aaatattatg ctttcagctt
      301 ttatattagt tacaataata cctggaactt ttttattttt ttcatcaact atgtatatga
      361 ttattgatgc tgttaagaag agaaaaagtt tttattataa gggacagaat ttaattgcat
      421 tttctgaact tggatttaaa ttaaagagca atagtagtgt attggctgta atagcaatat
      481 taatagctac aagtgtaact atgctagggt ttaccatatc actttattac gatatagata
      541 ggaacataag tgaaaattat aaatacagtt ataccataaa tttaggaaat tcttatgtaa
      601 ataacgaaat tgacaaatta ttatataaat ataaaaaaga taataagata atctttgata
      661 aaacaataga acttatagac aaagatataa atattgaaat gtattataaa aatggaaata
      721 gttatataga agatagaatg tctgtagata ttattagaga aagtgatttt aaaaaattaa
      781 gacaatatca gaataacaat tatgaggaat taacttcaaa ggaatctgtg tactatgttt
      841 ctgattcata caaaaaaata ttttttaaag actttgagat tgaaaaaata aacttgcata
      901 ttaaagatac aaatgagtat gatataaatc tgtttaaagt acaaaaaagc atcttggagc
      961 cagaaataaa ttcacaagct acgtttgatt taattgtagt aaaagatgac gtatttaatc
     1021 agttaaaatt acatggaaat tctagattaa taagattaat tgatataaaa aatgaaaaaa
     1081 aagaactgga tttgacttta aaattaaaaa atatagtaag tgaaaatatg aaatttactt
     1141 atccttttaa ttttacatca agtattgaaa gttataataa cttgataaaa ttaagtggtc
     1201 taatgctgtt tataggcata tttttgtctg tagtatttct tttatgtaca ggtagtataa
     1261 tcttatttaa acaattatca aatatatatg atgataagga aagatatatt atgttaataa
     1321 aacttggagc aaataataaa gatattgaga aaataatatc aaaacagtta aaagttatat
     1381 ttttaatgcc attagttgta ggtacagttc ataatttatt tgctatgtct atagtacaaa
     1441 agtttatacc aaggtcatta cttgtaccaa ttatcataac tctagtcatt tattttatag
     1501 gatattttat atattatttt ttaactttaa aatatgctct aaacatgata aaagaataaa
     1561 ttatttttaa gtcaaaagaa ttagatatat gttttgaata aaataactta tttaagtgca
     1621 gatagaaaat actagaattg agtttaagat atttatttaa agataaaatt aaagttagga
     1681 aaaagtctat attttatatc attttgtatc agttacatta cttgatgtta tataataaat
     1741 ctaagcagaa tatcaaataa aaaatgacga ggagctgaaa tttatgaagt ttaatgccta
     1801 ccaacataaa tactcatcaa taagaaatgt cgtgtatgct aaaaatggag cagttgcaac
     1861 atcaacacca ttagcatctc aagctggact tgaaatacta aaaaagggtg gaaatgctgt
     1921 tgatgcagca gttgcaacag ccgccacatt agcagtagta gaacctacaa gcaatgggat
     1981 tggaggagat gcatatgccc ttgtttggat agaggaagaa aaacgattgt atggtttaaa
     2041 ttctagtggc tttgctccag aaaatatgga attaaaaaat tataataata tgaaagaaat
     2101 gccaaaatat gggtttggat cagtaactgt acctggaata cccgcagctt ggtcagaatt
     2161 aaataaaaaa tatggtaaac ttagcttaat ggaatgctta tctccagcga taaactatgc
     2221 aagagaggga tacgtagtat ctcctaatgt agctaaggta tggaaaaaat catatgaact
     2281 ctatgaaaaa gaacttattg gagaagagtt caaaccatgg tttgatacat tttctaaaga
     2341 tggtaaagct ccagaggctg gagatatatt tatatgtgaa gaacaggcaa gtacattaga
     2401 agagatagca aatactcagg ctgaaagctt ttatagagga agacttgctg ataaaataga
     2461 tgaatattct aaaaaattta atggggctat aagaaagagt gacttagagt gtttttatcc
     2521 aacatgggta gaacctataa gtacagaata taagggatat aaaatatttg aaatcccacc
     2581 aaatggtcat ggaataacag ttttaatggc acttaacata ttaaaagaat tggaattaga
     2641 aggaaatata gaaaatgtag aagacataca taaaattata gagagcttaa aattagcttt
     2701 tgcagattct aaaacatatg taactgatat agagcatatg aaggtaaaga ttcaggaatt
     2761 actaagccaa gagtatgcca aaaaaagaag tttattaatt gacaataaag aggctttgta
     2821 tccaactgca ggagagccat attgtggtgg tacagtatac ctttgtactg cagataagga
     2881 tggaaatatg gtttcatata tacagagcaa ttacataaat tttggttcag gaattgtgat
     2941 accaagaact gggattgctt tacatagtag aggaaataat tttaatttag accctaaaca
     3001 ccacaatgta gtaaaaccat ttaaaaaacc ttatcatact ataatacctg gatttttagg
     3061 taaagaagat aaagcaatag gtccatttgg tgtaatggga gcttttatgc aaccacaagg
     3121 acatattcag gtgttaacaa atatgattga ttttggatta aatccacaag aggcgctaga
     3181 tgctccaaga tggcagtgga taaaaggaaa agaaatagaa gttgaaccag agatgccaaa
     3241 gcatataata gattcattaa ttgaaaaggg tcatgaaata aaagtaattc atgatactgt
     3301 tgacatggga cgaggtcaaa taatatttaa aacagaacag gaaagttata tttgtgggac
     3361 ggaatcaaga tgtgatggtc atattgcagt ttattaatat tatcaactaa aatatatata
     3421 ataaatttaa agagtatatg aaataataag taaaggcgaa agtctacaat gactgacgcc
     3481 tttacaaaac ttaagctatc atatgctgac tttatataga ttatgagcct tattcttgac
     3541 cagttttgat ttcattgtca tcataactaa tccagtcact aaagctacca atataaatct
     3601 tgtgaggtat atccagttct ctaagagcaa gactgttaac acaagcagat atccctgaac
     3661 cacaagataa aattacttca tcataattat ttaaatcttt aaagaatttt tttaaaaatt
     3721 ctatatcttt aagtgagcca ttttcaaagt cactttttat taaatcctta caaaaataat
     3781 tttttgcact tggtatatga ccagcttttg agtaagcagg ttcaactaaa ccctggtatc
     3841 tctcattagc acgacaatct attattatag tatctttttt gtataattta gacttaacat
     3901 attccattgg aacaactaaa tcattgttta tatttggtct aatttcctta cctgtacaac
     3961 ttggtatatt cactttctct tccaattctc caccttcctt tacgaaagat gttattcccc
     4021 catctagtac atagacattt ttaagcccca aatgttttaa ttggaaaaat aatctgcaag
     4081 ctccatttaa atctccatca tcataagtta caatcgtagt gtcgttatca acacccattt
     4141 tttctaattt ttcttttagt attaaagggt cttgtaaagg atttttacca ccatgttctt
     4201 ttgtaggaga tgataaatcc ttgtctatat ctaagataaa actgcctttg atgtgtcctt
     4261 ttttataaga atcaatacca taagttctat tgataagatc aaatctacaa tctattacaa
     4321 ctagattatc atttttttct agttttgata taagttcttt tgctgaaata atgtttttca
     4381 aagaatgtac ctcctgtgtt tatattatct ttattttaat ttat
//
LOCUS       DAGSHT010000251         4418 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.257, whole genome shotgun sequence.
ACCESSION   DAGSHT010000251 DAGSHT010000000
VERSION     DAGSHT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.257"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(81..428)
                     /locus_tag="MJB72_003382"
     CDS             complement(81..428)
                     /locus_tag="MJB72_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBY8264444.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(415..2343)
                     /locus_tag="MJB72_003383"
     CDS             complement(415..2343)
                     /locus_tag="MJB72_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBY8264445.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(2755..>4418)
                     /locus_tag="MJB72_003384"
     CDS             complement(2755..>4418)
                     /locus_tag="MJB72_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBY8264446.1"
                     /translation="LKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDLEF
                     VCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELDEV
                     TDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLNYL
                     LEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTISTSG
                     STGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVGAS
                     IDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERLKG
                     YRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPIGA
                     PGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLDYI
                     GRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEEDSG
                     KINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         1494 a          813 c          482 g         1629 t
ORIGIN      
        1 tctctatttt gagacagcct ctttcataat taccttctaa tttatatgct ctaatatatc
       61 tataataatt cttattatat ctatatatcc agtctttcaa tatagtgaca attaacttca
      121 atatgattta aaaatactaa caacctttca taaagtggat gagattcttc tccatattta
      181 gcttctaggt tcttgcctat ttctctaaca gtcctttgac catcaatttg taaaaaaaca
      241 aaactggaat attcatctag ttctattttt ttgtatttag gtattctagc ttttaacttt
      301 ctaaaaaact tttgaatttt atggtcctgt ttttcaagta tagtaacaac acgattttca
      361 gctacttcat attcaagtcc atcagtaatt ttaaatacaa tatctaaaac atcattactc
      421 ttgctcatta ttttttactt tagataaaac aataggtaat gctgtaagca caactaaaac
      481 tactaataaa gcaattgcca ttgaatttgt tgcagcaaat ccacttggta cctttggagt
      541 tacaactcca gttacttgta atattatacc tattaaacca atgatagaac caccagcaac
      601 aagaccagaa gataaactta taccattaga aactttaact tctttttctt tttcagtttt
      661 agatactaat tcaacaaata aacgaattaa tgctccaact aatattatag aagtagttgc
      721 aattggtaaa tagaatccta tagctatagt cattattggt aaatttaatg catatagaac
      781 tattgccata aatactccaa caataatcat aacccaaggt aaactacctg acataatacc
      841 agaagttaat gttgacatta agtttgcttg tggtaatgca aattgtacat tatcaccagt
      901 tgctctaagt tggtcagaaa gtaataaaat tactcctaca acaactataa caccaactat
      961 acttgcaatt gtaaagtaac gttgcatttc gtttttgcta ccaccaataa tataagttac
     1021 tttttgagat tgagtataac ctccagctat agcaatagca acaactataa atgaaccaaa
     1081 taaaagtaat gatttattag cttctaaatc agtccatccc attataacaa acactaatgt
     1141 tacaataact agagaagcta tagtcatacc agaaactgga agatttgatg ttccgattgt
     1201 tcctgttaaa cgaccagcta ctatgacaaa taataaagat aataaaagag atatgatagc
     1261 tccaataatt gccataacaa tgttaccaga aattaagaat gctgctaaaa atccaataac
     1321 tactccacct aataaaagta tcatttgtat agatgaacct tcttctcctt ctccagcact
     1381 tgatttagct ttaagtgttt cttttataga agcgattata gttggtataa gttttattgc
     1441 accaataata cctccacaaa gcatcatacc agcacctata tattttacat aactactaga
     1501 tattgctcca acatccattt ggttaagagg cattgcagga ttattccata ccatagctcc
     1561 atctttagcc atatctgtaa agtatcctat taaaggtgct ataccaaagt ttgataaaat
     1621 acttcctgca aacattgtta aagaaacttc aagccctact ataaatccaa ttcctaaaag
     1681 tagaggattt acttcaatat caaatttcca tttgtagaat ttgcttccta caagactcat
     1741 tacattgttt gctacattta agaatgaacc agttaataca gtaataaatc cacttattac
     1801 aaatccaatt cccatgtact taattgaatc tccaccagcc tctgaagcaa caagtgtctc
     1861 agatatagcc attgattctg ggtacataag tttaccatgt tcttcaacta ttagatagtt
     1921 gtgaactata gcagcaattc ctataccaaa taaaactcca ccaactccaa cagctaagcc
     1981 ttctaagaat gtaatttggc ttccaattaa tataactgct ggtaatacga aaataaatcc
     2041 acttgcaaca gattctccac cacttgacat accttggatt aagtttttac caagaatacc
     2101 ctttgaacga gcaaatgctc caacaaaagc agaacctatt atagctcctg gaatacctgc
     2161 ggcaactgta agaccggctt tcattccaga gtaagttgta gatgcggcaa aaatggcagc
     2221 taaaataata ccgataatca gtacagctac atttccaccg gttctggact tatctgtgat
     2281 atatggaacg taatcttttc cactaacctc accataagca cccttaggta attttttgtt
     2341 cataaaattt tcaccccttt tcaatttgta tatattataa catatataca tataaaactt
     2401 aactttttat tattttacaa atagcgagtc aaaactacta aaatttaacc ctatattatt
     2461 aatttctact tttagatatt tttatgctaa ttttcttttt atttttcaat tctactaaaa
     2521 actattaaga tataaaataa aaaaattttt taatgtaaaa ttaattttgt aaaaaaaatg
     2581 ctttaaaaca cggaaaagta taaaaaatac aataatcatt cttataaata aattttcacc
     2641 atagtaaata tatacagaaa attcttgttt ttatgcaaat tgattatttt ataaaaataa
     2701 gtgtcataaa gaaatgtctg aaatcacttt atgacaccta tcataataaa aaaattattc
     2761 tggtaatgct cttttattta cttttccgat tggtgtaact ggtatcttat ctatcctcat
     2821 tattattgtt ggaatcatat atggaggtag atgctcattt aaatattctc taatagttct
     2881 ttcatttatc ttaccactat cttcctcatc aattacataa tatgcacata tataaatatc
     2941 tccatcatca tttgttctcg atatacaaac aacactttta atttctgtaa tattatttat
     3001 tatattttca atctcatata gctctatcct aaactcatca atttttactt gattgtccat
     3061 ccttccaata taatctaaat ttccatcatc taaccatctt actaaatcac cagttctata
     3121 cattaactta ttatgttcac aagttttgta aggattctct acaaatactt gctctgttag
     3181 ccctttttta tttaaatatc ctcttgatat ttgaatgcct gatagacata gctctcctgg
     3241 tgctccaatt ggacatagtt tgttcttttt atctaataca tatgcataag tattaaatat
     3301 aggttttcct attggtattt ttccatattc tctgtccaca tgaaaagaag tcgatacaac
     3361 agtaaattcg gttggtccat actcattata tagttcataa ttacgctttc tatatccttt
     3421 taatctttct cctccagtct gtacaactct aagtgaatta ttattgcaat tttgtataaa
     3481 taaccttgca aatgctgttg gcaatgctag tatagttatg tcattttcct cacagtattc
     3541 atttactgta tattcatcaa atttagcttc ttcaggcata atatctattg atgcacctac
     3601 aagcaaataa gggaatagtt gtttcattac agcatcaaaa ctgaaactta agtatatacc
     3661 acatatatca ttttctgtag cgcttataga ttttacgcta taataacaca tatttactaa
     3721 actcttatgt tctatagcta tggtctttgg cctaccagtt gaccctgaag tagatatcgt
     3781 atatgcacaa tttgatgatg ttattttttc agttaattca tatccaaact taaacatttc
     3841 ttcatcattt atatcgagta ctatggtatt ctcatttaca accttacttt tgagttcatc
     3901 tgtagttatc actactttcg cattactatc ttcaagaagg taattcaatc tttcttctgg
     3961 attacttgtg tctattggaa aaaatgctcc ccctgatttc ataatcccta ttgctgtaat
     4021 aattatgtta atatctcttg gtaacattac agctactatg tcttcacttt ccactcctat
     4081 atgatttaaa taacctgcaa gtttatcagt tacttcatcc aattctttat atgttaactt
     4141 tctattttca tccctagcag ctatattact aggtgttacc ttaacttgct ccctaaataa
     4201 atcaacataa cttttatcat agggtacttc atattgatta ttattaaagt cttccaataa
     4261 tactttttct tcttccttac atacaaattc taaatcttct attaaaatat cattatcttc
     4321 tattatttgc tctattatat aatcaaattg atttaaaatt cttctaatag tttctctttt
     4381 atatatagat ggtttatact ttatttttat tttaagtg
//
LOCUS       DAGSHT010000252         4412 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.258, whole genome shotgun sequence.
ACCESSION   DAGSHT010000252 DAGSHT010000000
VERSION     DAGSHT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.258"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            376..1188
                     /gene="modA"
                     /locus_tag="MJB72_003385"
     CDS             376..1188
                     /gene="modA"
                     /locus_tag="MJB72_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY8264447.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1198..1989
                     /locus_tag="MJB72_003386"
     CDS             1198..1989
                     /locus_tag="MJB72_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264448.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1991..2686
                     /locus_tag="MJB72_003387"
     CDS             1991..2686
                     /locus_tag="MJB72_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8264449.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMSVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2673..3404
                     /locus_tag="MJB72_003388"
     CDS             2673..3404
                     /locus_tag="MJB72_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264450.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLERLCFCSK"
     gene            3718..>4412
                     /locus_tag="MJB72_003389"
     CDS             3718..>4412
                     /locus_tag="MJB72_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY8264451.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVD"
BASE COUNT         1738 a          536 c          627 g         1511 t
ORIGIN      
        1 ctttcttttg tattcaattc tgatataaca cttttagtaa tttgttaata aaaaacaatt
       61 aaaacatatc tatatgtttc aatgttctat tttttcactg aaatataata aatataaaat
      121 tagttaaaaa gtaaaaaatc tacaataagt taatttgatt taagttttta atttttatta
      181 atactttaaa gtattaataa aaattaaaaa taaaataact acctatggaa atgacattat
      241 aaatagattt tttcttaact atacataaca aaacataaca aaacattata aaacataaca
      301 aaaacttaga catcgttttc ttagtatgat atactaaaaa agaatttaca ctaatttact
      361 aagggggaat atcttatgtt taaaaaactt tcagtactag ctttagctat gattatttca
      421 ttaagtatta ctgcatgtaa ttctaaagaa aacaaagcta atgatgaatc taatactaaa
      481 gtgacaacag ctgatgattc aaaagaaaaa gagactcttt ttgtctattc aggggcaggt
      541 ttaaagaaac caatggatga aatagcaaaa aaatttgaga aagaaaataa tgtcaaagtt
      601 gaatatagct atgcaggttc tgcacagcta atagctcaaa ttgagacaag tcacaaagga
      661 gacgtattta ttgttggttc tgaaccaatt tattcaaagg cctttgaaaa agaacttgta
      721 ggtgaacata aaacggttgc acatcataca cctgcaatag ttgtacctaa aggaaatcca
      781 gctaatattc aaaaattgga agacttaaat aaagatggag ttaaacttat tttgggagac
      841 aaagagtcta atgctatagg taagactaca caaaaaatac ttgaaaaaaa taatttaaaa
      901 tctataaatg ataatgttgt ctctacagct gcaactgtaa atgaaatgat tgtacagtta
      961 acatcaagta aagctgatgc tacaatagcc acaaaagatt cggtctttgg taatgaagat
     1021 gtagaagtta tagaaattgc ccaagataaa aatatagacc aacttatatc agcaggaatc
     1081 gtaaactata gtgataaaaa agatttagct aataaattta taaattatat agagtcagac
     1141 gaagctaaac agatatttaa aaaatatggt tttgaacctg ttaagtagtg attaaacttg
     1201 aaaaataaat tttttactac tctacttact ggattttttg ctatagtctt tttgtttatt
     1261 gtatcaccac ttcttatgtt aatcattaaa gggataagtt atgttcctat atgcttaaaa
     1321 tcagtggaag tacagtatgc aataacactt agtattaaaa catctttaat atctactata
     1381 gtatgccttt tattagctat tccagtagca tattttttac atattactaa attaccattt
     1441 aaaaaactga ttatacagat aataaatctc cctatgtctc ttccacactt agtatcagga
     1501 attgctcttt tacttttgtt tggaagaatg gggattggag attccattta taagattttt
     1561 aagcttgatt ttatatttac aaagcaaggt atagttttag cacaaatatt tgtaaatctt
     1621 ccacttacta ttaaaatact tcacacttca ttaaatgaaa gtaatgaaaa gatgatattt
     1681 gttgcacgta cccttggatg taatagttgg gaagcattta gatttataat tcttccaaat
     1741 ctaaaaactg gaatcatatc tgcaactgta atgacttggt caagagcact tggtgaattt
     1801 ggtgcagtag ctatgatagc aggttctaca cgtatgaaaa ctgaaattat tcctacatct
     1861 atatatttaa atatgtctac tggtgatata gacattgcaa tcggaatagc tgtaattttg
     1921 atatttatct cacttacatg cttgatgctt tttgagatat tttttaatag agaggttgat
     1981 aagaattaat atgttaaaaa taaaaaattt atctcttgat ttacctaatt ttaaattaca
     2041 agaaaatagt ttaaatatta ataatcatga atactttgtt ttattaggag ctagtgggtc
     2101 aggtaaaact ctattcctag aaacattagc aggaagatat ccatctgcta aaggaaatgt
     2161 atcatacaaa gaaaatttaa tatcaaactt acctccagaa aatagaaata taggatttgt
     2221 atatcaaaat tttgaattat ttccaaatat gagtgttgaa gataatataa ggtttccact
     2281 taaattaaga aaaataccta tagatgaaca gattcttaag gctaaccact tgtctaaatt
     2341 aatgcagata gaagatataa aaaataggta tcctaaaaat ttaagtggag gcgaaaagca
     2401 acgagttgct tttgcacgtg ccttaatagc tgaacctgat atattacttt tagatgaacc
     2461 tatgagttca ttggattata ttacaaaaaa acatataagc aaaattttga aggatgtcta
     2521 cataaaatac aaaccaacag tgattcatgt tactcatgat atttctgaag caatgttttt
     2581 tgctcataag ataggaataa tgaacaatgg aaaaataaat catatttttg atttaaatga
     2641 agaaataaaa aataaaggag aatctttttt ctatgaatat atttgatatt ttgagagatt
     2701 atctattagt acaagtcgat aaatataatt taaatatgga tatgataagt attgtgtcca
     2761 aatctctatc ttcaaaagaa gcaattggaa atactaagcg taaagatttt ccaattatag
     2821 ttggtaaaga aataatgctt gaagctgact ttaaaggagc taaaggacaa gcatttactt
     2881 ctactccatc tacatttgaa ggttctttaa aagacatatt atcattagat ttacatgaca
     2941 atcctcatga tagaagtctt tttatagcat cattaaatgc agttatgaaa tacttaggca
     3001 agacggatag aaccattcat tgtaaaaata acgagccaga ggtatgtgca aaaaaattcc
     3061 ctgagtttat aaaaatggaa tttggtaatc caaaagtagc aattatagga tatcaaccag
     3121 ccataattga taatattaag gactttttcg aaacaagagt attagattta aatcctgaat
     3181 ttgtggatac tattcaatat aatgttaaga ttgaagatgg tatacgagat tatgaagatg
     3241 taatatcttg ggctgattta gtgatttgca caggaagtac attatgtaat aactcaatta
     3301 taaattttct atcacttaac aaaccagttt actattatgg aactacgata gctggtgcat
     3361 caaatatttt aggtttagaa agactctgtt tttgtagtaa ataagcaatc atatcatcaa
     3421 ggagtacaca aatatatatg tgtactcttt aaatttaaaa agttattact tttattctaa
     3481 gtactaaagt aaaattctat taaaatgaat cccgaataaa tgtacaatcc ctttaaatct
     3541 caattcctat caatcattaa tattttgatt agtcttatac ataaatgttt tctttatttt
     3601 aaactaataa aaaaatttta ttccattaat ctactctata tttttcagca tttaagaaat
     3661 aatttttagt actttgtttg ataatataac taagacaact tttcaggaga tgattaaatg
     3721 acatttaaga ataaaaaatt tactataaat aaatataaaa ttctatctat tattctagtt
     3781 ttatgtttga ttttaaccgc ttttagtagc tatgtgaatt atagtcataa aaaatttaaa
     3841 gattatgttg tcttctatgt tcagcatcaa gatgatgaag tgttgtgggc tgggagtgct
     3901 attgttaatg ctataaaaga aagaggaaaa aatcatgtat ttgtggtttt agtatctact
     3961 ggatgtggta tatccgtctt tgataaatat aaaaaatatg aaaacctaac aaatatacaa
     4021 aaatcagaat atagaaatag agaattttta gcttctctta atagccttgg tgtaataagg
     4081 gaaaatataa tattattacc agaaacaaat acaaatggta atactgactt caattacatg
     4141 gaaaaaattg ctcttacatt tgaagaaaaa tttaaaaatg taactcatat agcacatact
     4201 tataagttag atgaccattt acaacattta aaaaatggtt ctgttatcca aaatttatat
     4261 aatgctggta aaataaaaga tgctaaatat tttgtaaaac ctaaatttgc tgataaaata
     4321 acttttaata ataagatagt ttataaagct cattctactg aagactatga aaaggttaaa
     4381 aatgcctgtc aacaatacaa aattgttgat ga
//
LOCUS       DAGSHT010000253         4350 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.259, whole genome shotgun sequence.
ACCESSION   DAGSHT010000253 DAGSHT010000000
VERSION     DAGSHT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.259"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            102..1730
                     /gene="aspD"
                     /locus_tag="MJB72_003390"
     CDS             102..1730
                     /gene="aspD"
                     /locus_tag="MJB72_003390"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBY8264452.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1851..2846
                     /locus_tag="MJB72_003391"
     CDS             1851..2846
                     /locus_tag="MJB72_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBY8264453.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3024..3407)
                     /locus_tag="MJB72_003392"
                     /pseudo
     CDS             complement(3024..3407)
                     /locus_tag="MJB72_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3634..3731
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3872..4297
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3872..3901
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
BASE COUNT         1554 a          599 c          652 g         1545 t
ORIGIN      
        1 actttttcta taaacctatt ataattaaag tataatcatt tttgcaactt gtttatttta
       61 ttataaaaat gtgaaactta ttacataagg aggaatttaa catgaaaaat catacttata
      121 aagaaatcaa aaacatatat ggaaaaataa gcccttttga atttaaggac aaattaatag
      181 atattgcaaa atacactgca aaagaaaata acagagaatt acttgatgca ggtcgtggaa
      241 atccaaactg gacttgttca actgctagag aagccttttt tacttttggt catttcgcta
      301 ttactgaaac tcgttctaat tgggatttag gtcatttggc tggaatgcct caaaaaaagg
      361 gtattaaaga aagatttttt aaatttataa atgaaaatat agatatgcct ggagcatatt
      421 tagctagaga tattataaac tttggtataa atgaacttgg atttgatgga gatgaatttg
      481 ttcatgaact agctgatgga attattggtg ataactatcc acttccagat agaatgttac
      541 ctcatatgga aaaaatagta cacgattacc tagttcaaga aatgaaatat gatatatcag
      601 gtaaatatgg tgatgtagaa atttttgctg ttgaaggtgc tacagctgct atgtgctata
      661 tatttgattc attaatggca aatgaacttc taaaaaaagg agataccata gccttaatga
      721 ctccaatatt tacaccttat cttgaaatac ctaaccttcc tcgttacgac tttaaagtgg
      781 taaatataaa tgctaatgaa gttgatgaaa aaggtgctca tacatggcaa tatacaaaaa
      841 aagaattaga gaaacttcgt gacaaatcta ttaaagcttt attcgttgtt aatcctaata
      901 atcctgcttc tatagctatg gatgaaacat cttgtaataa tctaatagat gtaattgaaa
      961 attataataa agatttaatg ataatatcag atgatgtata tggaactttt gtagaagatt
     1021 tttcatcctt aatgtcaaaa ctaccttaca acactgttgg agtatattct tattcaaaat
     1081 attttggagt tacaggatgg agacttggta catttgccct tcataagaaa aatgtttttg
     1141 ataagaaaat aaatgattta actggagaat taaaaaaatc agtagataag cgttacagtg
     1201 atatgagtct taacccttct agcctttcat ttatggaaag agttgttgct gatagcagac
     1261 ttgttgctct caaccatact gctggtcttt ctactccaca gcaagtacaa atggcgtttt
     1321 tctctgcttt tgcattgatt gacaaagtag atgcctacaa aaaactcaat atgagtatct
     1381 gccatagacg tcaaaaacta ttatttgaag ctttagagct tccaattaat gaaaacaaaa
     1441 acaatgctgc atattacact caatttgata ttgaagaatg ggctaaatta aactacggag
     1501 aagacttatt taaatttata agtaaaactg catctccagt agatgttctt tataaattag
     1561 ctaatgacta ttcagtagtt ttacttagtg gaaatggatt ctatggtcct gaatggtcaa
     1621 ttagaatatc tctagcaaat ctatatgatg aagcttatac taagatagga aaagccataa
     1681 gagagatatt aaatggttat atcgttgaat ggcaaaaaat taagaaataa ttatattaca
     1741 ctgaaatatc taaattatat gaataaaaga gtttatgtag accttaacta ctttttttga
     1801 tataatccta tcggaattat aaattttatt tatgaaaggc agagaatagt atgagtatta
     1861 agaaaaaagt tgccatagtt tttactggtg gaactatatc tatgacagta gatgataaag
     1921 tcggagcagc tataccaact ttatcaggtg aacaaatact atcaatggtt accaacattg
     1981 ataaagttgc agatgttgaa gtttttaatt ttgatgaaat tccaggacct cacataacac
     2041 cttatagaat gatggaattg aaaaattatg taaatgattt attagcaaga aaagatatta
     2101 caggtgttgt aataacacat ggtacagata gtttagagga aacagcttat tttctagacc
     2161 ttacaattga caacatcaaa cctgtaatag tcacaggtgc tatgagaagt agttctgaac
     2221 ttggatatga tggttcaagt aatttatctg catcagtttg tactgcagta tccaaagatg
     2281 ctatgggaaa aggagtcctt gtagttttaa acaatgaagt attattagct tctgaagcta
     2341 ctaaaacaaa tactctttct ctaaatacat ttaaatctct aactagtggc ccattaggaa
     2401 taattgactg caacgaactt gtccttacaa gagatattgt aaatagaaca attatagata
     2461 ctgataaggt agaatctaaa gttgccttat ttaaagcata tgtaggagaa aatgctgact
     2521 ttataagatt tgctgttgat agtggttata aaggtatagt tattgaggca atgggtagag
     2581 gtaatattcc tcctcaaatg ctttctggag ttgaatatgc tagagaaaaa ggacttcctg
     2641 tagttatagt ttctagatgt cattcaggta gagtattcga tagttatggt tactttggtt
     2701 ctggtagaga tttaaaaaat cttggttgta tatttggtgg ggacttacca ggtcaaaaag
     2761 ctagaataaa acttatctta gcacttggaa aaactgataa ccttgatgaa atcaaagatt
     2821 tctttgaaaa aggaatctat tgttaattct aataacttct atttaattat ttattgatat
     2881 ttcaacattt ttaaatcaaa atctaatact tatgtcagtt aaatgtcagc aaaatatttt
     2941 atccacaagt tttccacaac ttatccccaa tattatccac aagaaaaaag gtagcactta
     3001 tattagtagc taccttttat ttattaatct tcatctttcc agctttttat ccaatttcct
     3061 atttctatta ttttttcttc agttatttct tctccatcat gcaattttga aattaattct
     3121 gataatggat tgttatgtag acctccaaaa attctttttc cctctatttt gagatattct
     3181 ttctctttta ttaatactct atagtgtaca tgtttcccaa ttctctgcga tatgaaaaaa
     3241 ccttttttag ttaatctagc taaaagtgta attgtagttg tatgcttcca agcatatgtt
     3301 ttttctattt catctgcaac atcttttgat attacagttt tataaccagt attctatatg
     3361 tatttcataa tttttaattc tgcatctggt aattttttat tcaacataat atattccttc
     3421 tttcaattta tgtcacttcc tcaaacttat ctagcttaaa ttaataatag gagcttttta
     3481 tcacaaaaac tacaggtaaa tttttctata tattctacgt atttagtctg ttttttaagt
     3541 gtgaaattac gttcatacct ataaatttat tcaattttca aacaatctta ttgaataata
     3601 actaattcta ttgtattatt tagttataga ataaaactaa atcggcaaaa ctagagaaat
     3661 ctagtgacgc aaagctatag agactaagac ttatataaat atcttatgag ttatgtcagc
     3721 cagttgccaa agagatattg ttctttttgt ttttatgaaa gttttttagg gggataataa
     3781 tttatatatt tagagataaa atagataaat gtacacatat attgactgct tatattagca
     3841 gttcgtatga ttattgtaat tttttggata caatttacat tccatatagt taatataaaa
     3901 caagtgtttc gaatttcttt tctgaaacta taccaacaat ttacattcca tatagttaat
     3961 ataaaacgtt atatactgaa agcggtgaaa ctggttacgt agcaatttac attccatata
     4021 gttaatataa aacctgtgcg tgtaggagat ggtaacacaa ctgttgaaga atttacattc
     4081 catatagtta atataaaaca cacatctaca tgtttttcta tctgagttca tctctaattt
     4141 acattccata tagttaatat aaaacttttt aatccttctt ctgaactccc aaagtttata
     4201 tcatttacat tccatatagt taatataaaa cccacgaaat ttaaggtcga tatatcagat
     4261 ttgaaaaaat ttacattcca tatagttaat ataaaacaag ctaaatggag caaccgacca
     4321 agtcgctaga aataatttac attccatata
//
LOCUS       DAGSHT010000254         4344 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.260, whole genome shotgun sequence.
ACCESSION   DAGSHT010000254 DAGSHT010000000
VERSION     DAGSHT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.260"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            189..1643
                     /locus_tag="MJB72_003393"
     CDS             189..1643
                     /locus_tag="MJB72_003393"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBY8264454.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            1914..4193
                     /locus_tag="MJB72_003394"
     CDS             1914..4193
                     /locus_tag="MJB72_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264455.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
BASE COUNT         1763 a          441 c          725 g         1415 t
ORIGIN      
        1 ttaagtaccc gattttatta tccatagttt ttatagaaga cctttcctca gttatattat
       61 agaaaattat agagcatttt ttgcaatgtt tcaagcattt ttaaccattc acatagtaaa
      121 aaattcttaa tataatatta tatgtagtgt aattttagca gaaagagttt aaagggagga
      181 aagcatttat gaaattaaat gatattatac aggggttgga tataatcaat gtcaagggtg
      241 aattaaatat agatattaat aatgtacaat atgattcaag aaaggttaca aaaggtacat
      301 tatttatatg tataaaagga tttgtgtcag atggtcataa gtatataaaa gatgccatag
      361 aaaagggagc aagtgcattt cttgttgaag aagatgtagc aataaaagga tgtacattta
      421 taaaggtaaa agatacaaga aaagatatgg caaaagtagc tgataatttt tacaatcatc
      481 catctcaaaa atttaacgtt ataggagtta caggtactaa tggaaaaact agtataacta
      541 ctatactaaa tgagatatta actttaaata agaataaagt tggacttata ggaactataa
      601 aaatttttga tggagaaaaa gacattgttt ccaattcaac aactccagaa agtatagatt
      661 tacaatatca ttttaataat atgttggata atggatgtga ctactgtgca atggaagttt
      721 catcacattc attggcatta aatagagttg atgaaacaga ctttaaacta ggcatattta
      781 caaatttaac accagaccat ttagattttc ataaagattt agaggattat agaaaagcaa
      841 aagaaaaatt attttttaag actactatgg caaatataat aaacatagat gatgaaggtg
      901 gaaagaagat atatgaaaat ataaaaggta taaatgtacc ttgttacact tatggtgttg
      961 atactaaggc tgattttatg gctagagata taaaatcaga ttcggatgga gtatcttata
     1021 gattaataac accttcatat gaagaagtaa tatttatacc agttccagga atgtttacag
     1081 tatataatac tttagcagtt atagctgctt gctatgtact tggcatacct aaaccaatat
     1141 ataaagaagg ccttagactt tctaatggag tatcaggaag gtttgaaaca gttccaaatg
     1201 ataaaggtat aagcgtaatt gtagactatg ctcatactcc tgacgcactt gaaaatgtac
     1261 taaagacaac tcaacaattt gcagaaggaa aaataatttc tgtgtttggg tgtggtggag
     1321 atagagatac tgaaaagaga cctttgatgg gagcaattgg acaaaaatat tctgatttat
     1381 gtattataac ttctgataat ccaagaactg aagaaccaga ggcaataata aaagatatac
     1441 ttgaaggaat tgacaagaaa aaagaaaatt accatgttgt tgttgataga gaacaagcta
     1501 tagcagaagc tataagtatg gcaaaaaagg atgatgttgt aattataaca ggtaagggac
     1561 atgaaactta tcaaattata ggtaaagtta agcatcattt tgatgataaa gaagttgcaa
     1621 atgagtgttt aagtaagatg tagcataaaa attaaacaaa atacaagaac aaaaatgtaa
     1681 tatcaagctt cactttgata ttacattttt ttatatgtaa ataattgtaa aatagataat
     1741 gcaataatat aaacactaat atctatatta attaatttat tgttgtaata aagtttataa
     1801 ttttgtataa ttacagtaaa tatggaaaaa taagtattat tataaaatgt ttacaataat
     1861 tgtcataaga taatgattat acatacatag attcgagaaa ggcaggaagc ataatgttaa
     1921 gtgatgcaaa aaataatagg tatggtttgt ccataattgc tgtcataagt atcttagtaa
     1981 ttacaatttc ctgtttgatt tatggttcaa ggttgaataa gacattaggt gaagaaacga
     2041 atcaatatct ttcagaaata gcaaatcaaa gcgtcaatgt gttaaaaaaa caaattaatg
     2101 gagatataaa gttactagag tcaatttcaa tatttattga aggagaagag tcttttgaag
     2161 tagataatat cctttctata ctaaaaaggc aagctattaa tagttcattt aaaagaatgg
     2221 gagttatttt accaaatggt acagcataca ctacagatgg ttatatagaa gacttttcta
     2281 aaagagatta tttcttaaaa tcaatgcaag gagaagtggt tataacaggc aaattaaaag
     2341 atgtaataga agatgataaa aataccaata gtaatattaa tgtatattct gtaccaattt
     2401 ataaagataa tgaggttaaa ggagttatat ttgccacaca tagtacgaag ttgtatgaaa
     2461 aaatactttc tgtacctaca tttaatggaa aaggatattc ttatataact agaagtaatg
     2521 gtgatattgt tttaaatcca agcagtaaaa acgccaactc tactaaagaa aatttatttt
     2581 cttacattgg tagaagtgat tcaatatcaa aatctagtat caataatatg aagtcaaata
     2641 taaaaaactt taaatcaggt tcattaagtt atagtatgtt tggaaaagga tattatttaa
     2701 gttatgcccc aataggaatc aatgattggt atcttttttc tgaagttcct aaaactgcta
     2761 tatctgagaa atcatatgct attataaaac ttacattatc agcatgtgta gtcttgatta
     2821 taatctttac ggcattaaca atttatatac tatttataca taaaaagagt aaggaaaaat
     2881 tggaagaatt tgcttttaaa gataacgtta caggtattgg caattcaaat aaatttaatc
     2941 ttgaaggggg aaaattttta tcctctcatg agaaaaagaa tctagtttta atttattttg
     3001 atatagataa gtttaagcta gtaaatgata ggtttgggta tgaagaagga gatagagtat
     3061 taaaggaaat agcagagata attaagaata tatttaaaga gcaatctgta ttttcaagaa
     3121 tttcaaatga caattttgct ataatatttg aaaagaaaaa taatagagag tcaattatag
     3181 aaatatgtga acttatacgt aaaaaattgt caatgataaa aacaagtttg ggagtagaac
     3241 tcaatttaat tccatccata ggtgtatatt tcgttgaaga gggagaaaca aatatttcta
     3301 cctgtcttga caaagctatg attgcaaaaa ctacagtaaa aagaaaatat ttaaaagttt
     3361 atgagattta tgctgaaaat ttaaaagaaa ctctcataga agaacgtgat attgaacaag
     3421 aaatgcatga tgctttaaaa aatgaacaat ttaaagttta tttacagcct aaggttgatt
     3481 tatcaactac taaaattgta ggtgctgaag cacttgtacg ttggcaacat cctaaaaaag
     3541 gtctaataag tccaggtgta tttattccta tttttgagaa aaatggtttt ataacagaat
     3601 tggatatgtt tgtatttacc caagtttgta aaaattttaa acgttgggaa aatgaaaatt
     3661 tccccacatt tcctatatca ataaacttat caagagtaca tcttgagaat ccaggcttta
     3721 tttctgagct tgaaagaata acaaaagaat atgaaattga accaaattta attgagattg
     3781 aattgacaga aagtgcaata tttgacaata caaaaatatt atttaaaata atgcaaactt
     3841 tgaaatctgt tggatttaaa atttctatgg atgattttgg ttctggttat tcttctttaa
     3901 atatgcttaa agatatgcca attgatgtat tgaaattaga taggcaattc tttataacag
     3961 tgggaaatgc aaaaaagagt caaattgtag tttcaagtat tgttcaaatg gcaaagcaac
     4021 ttgatataaa agttgtttca gaaggtgttg aaactgtaga gcaagcagag tttttaaggt
     4081 ttataggttg tgatatggct caaggatttt tatttgctag accaatgcca atagaagaat
     4141 atgaaaaatt aatattagat agatatagtt taaatagaaa agaaacaaat taaataaaat
     4201 tgtgaattat aacataaaaa tttataaaaa agtgaacttt gttaatcaat gttcactttt
     4261 ttatgtaaaa atatactatt ataaaaacat aaatcattgt taggatatta ttttaggaat
     4321 tattgggcaa agtttattaa tatg
//
LOCUS       DAGSHT010000255         4288 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.261, whole genome shotgun sequence.
ACCESSION   DAGSHT010000255 DAGSHT010000000
VERSION     DAGSHT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.261"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            363..1574
                     /locus_tag="MJB72_003395"
     CDS             363..1574
                     /locus_tag="MJB72_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY8264456.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1767..2027
                     /locus_tag="MJB72_003396"
     CDS             1767..2027
                     /locus_tag="MJB72_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBY8264457.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2381..4093
                     /gene="ptsP"
                     /locus_tag="MJB72_003397"
     CDS             2381..4093
                     /gene="ptsP"
                     /locus_tag="MJB72_003397"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBY8264458.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         1767 a          395 c          769 g         1357 t
ORIGIN      
        1 attatgaaga gatagagaat ttatcaaagc aaataatatc aattttatta aataagtcta
       61 tagataaagg aaaagtagaa aaattgcaga taaaaatgga taacttgata atggattctt
      121 taggtatata agcagttgta agtattaaaa aattataatc ttttaattgt ttatttaaat
      181 ttagaaataa aaagttagcc atataaaaaa tattaaaaat atatactttt caatattttt
      241 ttgataaaat aagaatgaat ttgtgcaatt ttatttttct ccaataacta aaaatagttt
      301 tttgaattaa tatttgctta agaataaaaa tcagaaacgt ttttgatata gtaggtgata
      361 atatgaaagg attagtttta gaaggtggag gaacaaaagg agcctaccaa ataggtgcat
      421 ataaagcttt aagagagctt ggtatagagt ttcaaggagt aactggcaca tcaattggtg
      481 ctttaaatgg agcatatata gttcaaaatg atattgaagt aatggaagat atttggttaa
      541 attatgacta tactcatttt atgaatattg atagagaaac ctatgagagg tttaaaaatg
      601 tagatttcac tactaaaaat ctcaatgcag tgtttgagct tataaataaa gctagaaaaa
      661 atgaaggtat agatataagc ccattaagac aactaatgga gacaacatta aatgaagaca
      721 taataaggaa atcaaaaaaa gactttggac ttgttacggt ctggtgggat aaaaaaatta
      781 atccatatcc attgtatatc gaagatatac ctaaaggaag attgattgat tatttaattg
      841 ctagttccag tttgcctatt tttcaaacga acatgatgga tgataagttg tatcttgatg
      901 gaatgttttg tgataacatg ccaataggac tcttggaaga caaaggttat gaagatgttg
      961 ttgtaattag gttgattgat gattttattg ggaaaatgaa tctaaatcga catcaaaata
     1021 taaatataaa aactataata ccatcagaat atctaggagg ttctcttaat aaagatagag
     1081 ataatgtaga aaaaaacatc cgactaggtt acttagatac tatgaaagca tatgaaagat
     1141 gcactggaat taagtattat tttgatgtag agtataaata taatgaagaa tattgttttg
     1201 ataaatttag aagtatgagc aatgaaacaa tacaatatat ctgtaattta ttgaatataa
     1261 aaagagatgc ttcacttaga accgttatgg aaagtgtaat accaaagctt ggtgaggtgc
     1321 ttggattgcc aaaggatttt tcatataaag atttgtttta ctgtatatat gaaaagaaac
     1381 ttgaagaaaa taatataaac aggataaaat tatatgattt taacaaggtt atagatgttg
     1441 taaatacaaa tattgattct aatagaaatt tagcttctaa ttttgaagtt agacctaaaa
     1501 ttagtattaa aaagaacaaa aacacaaaat tattgacaaa ttgtattata aaagacataa
     1561 aaaatcaaag ttaaataaaa aacaaaaaaa aatttgtaat tatactcgaa aaaagtgtat
     1621 attagtagta taatattttt gtagcactag tgggaacaaa aaatgaccat aggacaaaaa
     1681 agtaccactt agtaccatta aagtaaaaaa caggaaaaca attttaaaaa atataaaatg
     1741 taaagtttat aggaggattt aaaaaaatgg aaaaagtagt tagtatcaaa aatgcaagtg
     1801 gattacatgc tagaccagca ggaatgtttg ttaagaaggc agctgaattt aaatcaacag
     1861 tagaggttgt agcaaaagac aaaactgtaa atgcaaaatc aataatggga ataatgagtt
     1921 taggtttagc tcaaggagaa gaattaaagt tagttgtaaa tggagaagat gaagaagtag
     1981 ctttagctgc aatggttgaa ttagttgaaa gtggatttgg agaatagata taataatttt
     2041 ttaaaaattt gatatttaga atctaatatt acttaaagta aagtaacaga gaagtattaa
     2101 acatatacag accaacattt gtagatattt aatttctttg ttactttcat gtttatttaa
     2161 tcagaagatt aagactattg ttaattgggt ataaaaatat aaatatttta gaaaatgaaa
     2221 taagatattt ttaatcataa attcagagta aaaatacagt aacaaatagt ttataaaatt
     2281 ttataaaaaa ttataagtaa ataaaactat aaaaatgtaa attatttata taaatttata
     2341 aagtttatgt gctagatgat attttttagg aggtagggtt atggcttaca aaggaatagg
     2401 tgcttctcct ggtgttgctt tagggaaagc gttagtagtt gaacatagtg aattagtaat
     2461 tgaaaaaaaa tctatagata atgttgaagc tgaaattgct aaattagaag atgcagttgc
     2521 agtttctaaa gaagaattag taaaagttaa ggaaaaggct tctgaagaat taggagcaga
     2581 ggaagcagaa atatttgaag cacatttatt agttttagaa gacccagagt taattggttc
     2641 tgcaatagat aaaattaaaa ctgaaagtgt aaatgcagag tatgctttaa atgaaataaa
     2701 agagatgttt gttagcatgt ttgagtctat ggacaatgag tacatgaaag aaagagcggc
     2761 agatataaaa gatgttacaa atagaatact tagacatata ttaggaatta aagttgttga
     2821 tttatctgct ttaagcgaag aagttgtatt aatagcacat gatttaactc catcagacac
     2881 agcaactatg aataaaaaaa tggttttagg atttttaact gatataggtg gaagaacttc
     2941 tcatactgct atcatgtcta gaactttaga aatagctgca atcgttggat taaatgatat
     3001 aacttcaaaa gttaaggatg gagattttgt ggttttcaat ggagacacag gtgaagtaat
     3061 cgtgaatcct gatgaagaga ctataaataa atatactgaa ttaaaagcta agtatgaaga
     3121 tgagagaaaa gcattacagt tattaaaagg aaaaccatct gtaactttag acggaaaaca
     3181 tgtggaatta gctggtaaca taggaacacc taacgatata gaaggtctaa taaagaatga
     3241 tgctgaaggt gttggtcttt atagaacaga atttttatac atggatagag attctttccc
     3301 aacagaagaa atacaatatg aagcatacaa agctgtatta gaaggaatgg atggaaagcc
     3361 tatagttata agaacattgg atataggtgg agataaagaa ttaagttatt taagtatgga
     3421 acctgaaatg aatccattct taggttacag agcaataaga ctttgcctag atagaaaaga
     3481 tatatttaag actcaattga gagctttata tagagctagt gttcatggaa gattaagaat
     3541 catgttccca atgatttcat ctttagaaga gttgttacaa gctaaagaag ttgtaaaaga
     3601 agttttagca gaacttgatt ctgaaggtgt ggcttatgct aaagatgtag aaattggtat
     3661 gatgatagaa gttccatctg ctgctgtgat atctgatgta ttggctaaac acgtagattt
     3721 cttctctata ggaactaatg atttaattca atacacttgt gcagttgata gaatgaatca
     3781 aaagataagt tatctttaca atcaatttaa tccagctgtt ttaagactta taaagactgt
     3841 tatagacaat gcacataaag aaggaaaatg ggcaggtatg tgtggagaat ctgctggaga
     3901 ccaaaaaatg ataccaatac ttttaggtat gggattagac gagttctcta tgtctccaat
     3961 atcaatactt cctgctagaa agttaataac ttcagttaaa gagtcagata tgaaaaaatt
     4021 agctgatgat gtacttaata tgggtactgc tgaagaaata aaaagctata tagaaaagac
     4081 ttttaatata taattaatgt tatatagtta ctaaaattca tttttctaaa taacaaaatt
     4141 atattttacg aataaaaaaa ctatagtata tcatatatac tatagttttt ttgttatata
     4201 aattacgtat caaaatttgt aaaatgaata gatataatta ctaaatatgt gatagtattg
     4261 gctataatag gatataaaaa ataatatt
//
LOCUS       DAGSHT010000256         4288 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.262, whole genome shotgun sequence.
ACCESSION   DAGSHT010000256 DAGSHT010000000
VERSION     DAGSHT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.262"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            49..1944
                     /locus_tag="MJB72_003398"
     CDS             49..1944
                     /locus_tag="MJB72_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBY8264459.1"
                     /translation="MLTLLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLL
                     NFIALFIIGYVFSYDLKRAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIA
                     QFVMNRNRMKNVGDKELISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINS
                     ISKNLKMVIFDNRKDDFYISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNI
                     LEESSINSKSDVLVVATKEINYIKMCLENKDTITDKEILGFRDYVISQDTKNINLKEI
                     YSVLENLYEFLVDYKKVKSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKI
                     ALATAVAGFIMDYFHLRDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVL
                     FSIIKDSTLRSLIVLLAGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISY
                     VALGAIIALIVNKFILPYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNL
                     LLIPSLIEDRLMLINAIYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKIL
                     RDTNYISNYNVDKYDEGRSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITK
                     VSIKEAA"
     gene            2158..3015
                     /locus_tag="MJB72_003399"
     CDS             2158..3015
                     /locus_tag="MJB72_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8264460.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            3112..3981
                     /locus_tag="MJB72_003400"
     CDS             3112..3981
                     /locus_tag="MJB72_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8264461.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1800 a          376 c          706 g         1406 t
ORIGIN      
        1 ttatttggaa gtaaaaatac acttataggt gtaggtatag taacagctat gctaacactc
       61 ttggaaagag atttaactat ttctccaatt aaaaatttac tgaagtattt agcaataaat
      121 ataatcttag gaatactatc attttttgca gtacaaaata tgtatttagg agttttatta
      181 aattttatag cattatttat aatagggtat gtatttagtt atgacttaaa aagagcagta
      241 tatgtaccct ttggtctaat gtatatattt atggtaagta tacctgtagg aaaaagtgaa
      301 tttcctatga gattgtcggc tttagctgtt ggagcagtta taataatgat tgctcaattt
      361 gttatgaata gaaataggat gaaaaatgtc ggagataaag aactgatatc tatatgtgat
      421 gaattattag aaaaaattag tcttttaaaa aatactatta atgatgatag ttctataatt
      481 aaatctagta tgagaaaaat agatagctgt aattatagaa taaattctat atctaaaaat
      541 ttgaaaatgg ttatttttga taatagaaaa gatgactttt atatcagtat tagaggtata
      601 gacataatga atattttatt ttcattagaa agaataagtt taatcctgga aagatacaaa
      661 aaggatagta aagaatttga agatgaagat ataaaaaata ttttagaaga aagtagcata
      721 aatagtaaat cagatgtttt agttgtagca acgaaagaaa taaactatat taagatgtgt
      781 ttagaaaata aagataccat aactgataaa gagatattag gatttagaga ttatgttatt
      841 tctcaggata ctaaaaatat aaatttaaaa gaaatttata gtgttttaga aaatttatat
      901 gagtttttag tagattataa aaaagtaaaa tcaagagacg aaaagaaagc tgaaagaaaa
      961 tcaaagatac ctcatgaatt taaacgacta tcaatatata agaaaaactt taatttaaat
     1021 tctataagat tttcttatgc agtaaagatt gctttagcta cagctgtagc gggttttatt
     1081 atggattatt tccatttaag agatggaaga tggataatgt taactgtctt ttcattaaca
     1141 caaccttacg cagaaaattg tatacaaagg tctagaaaaa gaatagaagg tacatttata
     1201 ggagctgtaa tatttattgt attattttca ataataaaag atagtacatt aaggtcgtta
     1261 atagttcttt tagcagggta tataaactct tatgtagttg attatagaaa attaatggta
     1321 tgtgtaactg tttcagctct tggttcagca gtagttatgg gagacccaaa tgttttaaca
     1381 ataagcagga tttcatatgt agcattagga gcaattattg cattaatagt aaataagttt
     1441 atattaccat atgatgctaa aacaggctat caacatgtta tagaaatgta taaaggaata
     1501 gttaaaaata taattgatga agtcaataag tctatagaaa atgtagctga tgtttactat
     1561 ataaaaaatt tattattgat accatcactt attgaagata gacttatgtt gataaatgct
     1621 atatataaag ataaacatca agaggatttt ttagaaaatc agaaattact aataagtaat
     1681 atgtataatc tatatataaa tgttaataaa aataaaataa aagatgaaga tgttgaaaaa
     1741 atattgagag ataccaatta tatttcaaat tacaatgtag ataagtatga tgaaggaaga
     1801 agtgtaattt tagagagtat tgtaaatact aaaagtctag gtgacaagat aatatgtcta
     1861 aacttactgc aaactctaaa tggtgtcaaa gaaatgtata gaataagtaa tataactaaa
     1921 gtttcaataa aagaagcagc ttagatatta aattatagaa tagccatata tttaaaataa
     1981 agtatatggc tatttgtttt ataaaatact aaaagaaaat aatatataaa aaacaaaaac
     2041 atatatttat gaattttata tgaaaatagt taaaatattg atttttttca ttggttgaaa
     2101 aaacaattta cttaggataa actataatag tattcaaata ttatgggggt tagatttatg
     2161 agtaaagatg taaaggtaaa gcgtaataag aaatatgata catttagtat aactatggaa
     2221 cttgagagac ctttacttta tgatgaggtt gagaacaaag atgataaaaa taataataga
     2281 aaatttaaca taaagaataa gatatttaag ggaataggta tactatttgt attgattttg
     2341 cttggctttt tcatatggat aaataaaaca tatgaacctc aaaagctggc aaaagaagct
     2401 ctagtcagca atagtaaagt tgaagtaact gtaaatgaaa acatttcatt tacaccaaaa
     2461 ggaagaaaag tttctaaagg tttaattttg tatccaggag ctaaagtaga aatagaatct
     2521 tatgctccac ttgcaagaaa aatagcagaa agtggatatg aggttgtaat cgtaaagatg
     2581 ccacttaatc tagctatact tggaactaat aaggcacaga aagttatgga ttcttacaac
     2641 aacatagaac attgggtaat aggaggtcat tcattaggag gagttgcagc atcaaatttt
     2701 gcacgtgata ataaattaat tgaaggcgtt gtatttttag cttcttatcc aatgggtgat
     2761 gagttgaaag aattaggaaa aaaagttata tctatatggg ggagcaaaga tggagtagtc
     2821 aattttaaaa gtcttgtaga atcaaaacaa aaacttcctg atgatacaac ttatgttgag
     2881 attgaaggag gaaatcatgc acaatttgga gattatggaa aacaaaaggg agataatgat
     2941 gctattataa gtcaagaaaa acaacttaat attactgcca atagtatcat taaattttta
     3001 aagaatatat cttaaactaa aatttaaaaa atataatttt ataagttatt atttaaagta
     3061 aatattaata atataataga atgaatattt tattacaagg gggattttaa tatggaatac
     3121 gatatgccac tttacagacc accaagtgag gcttatagtc ttataataca agtcacacta
     3181 ggatgttctc ataacaaatg tacgttttgt agtatgtaca aatctaaaaa atttaaaata
     3241 aagccactag aagtaataaa aaatgaaatt gatatattta gaagatatta taagcatgta
     3301 gaaagaatat tcttagcaga tggagatgca ttgataatac caatggagaa attaagagat
     3361 attattttat atataaaaga agtttttcca gagtgtgaaa gaattactat gtacggctct
     3421 cctaaatcta tagagaaaaa atctgatgat gaacttaaag aattaagaaa acttggtgtt
     3481 aaaatgattt atctagggct tgaaagtgga agcaatgaag tattagaaga tattaagaaa
     3541 ggttttagta gtgaacaatt aataaaagtt ggtagaaaag ttaaaaaaag tgggattaaa
     3601 ttatcagcca ctgtaatagc aggtcttgga ggaactaaaa aaacacatca gcatgcagtt
     3661 gatacaggaa aaatgttagg agcaatttca ccagattatg tgggtgtact tagtttaatg
     3721 gtagaaccta atactgaact ttacgattta cttaaggcag gagagtttac aatactagag
     3781 gataaggctg tgttacaaga aattaaagag atgataaaga atatagatgc taatgaaaaa
     3841 attgtattta gaagtaatca tgcatcaaat tatgctaatt taaaaggtgt attacctgat
     3901 gataaacaac gtctaattga tgaaattgat tactatctaa gtaaccaaaa gctaaaaaga
     3961 gaagaatata gaagactata gtttttgata tatcaatcta tattgatagg taaatactaa
     4021 gattatttag atacaaaaaa tcatgctaca ttaatagttg cttactattg atgtagcatg
     4081 atttttttat taaaaaacaa aatggtatat tcatatataa tatatcaata ataatgaaaa
     4141 tgtgaactag tttaatagaa ttttacctat tataataaaa acaggaattt aaataataaa
     4201 aagtaaagta actttttatt aagtaacttt actttttatt ttatttaatg ttgtattaaa
     4261 tttatatata ttaaaataat atacaaat
//
LOCUS       DAGSHT010000257         4271 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.263, whole genome shotgun sequence.
ACCESSION   DAGSHT010000257 DAGSHT010000000
VERSION     DAGSHT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.263"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(66..983)
                     /locus_tag="MJB72_003401"
     CDS             complement(66..983)
                     /locus_tag="MJB72_003401"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBY8264462.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            1191..2075
                     /locus_tag="MJB72_003402"
     CDS             1191..2075
                     /locus_tag="MJB72_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBY8264463.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            2288..4129
                     /locus_tag="MJB72_003403"
     CDS             2288..4129
                     /locus_tag="MJB72_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBY8264464.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKTEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSGTLICLLNFNQKELKDKKSK"
BASE COUNT         1717 a          459 c          710 g         1385 t
ORIGIN      
        1 attcattcct aaatttttat tccaaatttt tattccaaat ttttagaaat aaggattcca
       61 ttaaattatt catcaaaatt taaacgcaag cttaatctat cttctccaag aatagtattt
      121 tcaaaaacat tagtagcatt ttctaaatac atactaggta tgtctaaaga aggactaaaa
      181 tgagtcagta aaagttgttt gacatttcca agttttgcaa gattagcagc ttctctaaaa
      241 gtcatatgtt tgtttctaac agcttttgat atatctaaat catcaccata catggcttca
      301 catataaata aatcactatc ttttatgaaa tctggtattg attctatagg tcttgtatcg
      361 gttgtaagac ttatcttaac tccctttctc aattctccta agaccatatc tggagtgtat
      421 tgttttgaat ctaaaactat attttgacct tcttggagtt tgttccataa tattttagga
      481 acttcgtttt tagttgcctt atctatatca aatttagggt ttcttctaaa attaaaactg
      541 tatcctaaac actctataga gtgttctaaa ctgattgttg aaatttctaa atttcttaaa
      601 actttattat ctaaagaaaa attgccttga gggttttcta taatttttaa agtgtatggt
      661 acatattcta ctaaatttct catagaacgt atacaatcga ctattccaac tggaccaact
      721 atagttaaat cacttgtacg accactattc ccaattgttg ataaaagtcc taaaagtcca
      781 aatatatggt caccatgtaa atgggttata caaatcaagt ctatatcttt aaagccacat
      841 ttttttagct tcatagatac ctgagtacct tcaccacagt caattagtat ttttctacct
      901 ttatagttta taaatacaga ggatagaaat ctgtttggca taggtacatt tccaccacat
      961 ccaagtaaag ttaaatctat cattttttcc cctttctaaa attaacacaa atctgttgtt
     1021 tgtttttatt atgatatcct attataatta ttttaacatt aaatataaca ataaatgtgt
     1081 atttgagtca agaaatattg tcaaataaag tttttatata gaaatgtata aaaatttgga
     1141 aaacaaaaag tgaaaattat atttaggagc atttaaagga ggaaaattta atgaaagtat
     1201 taaagaaaat tactattttg tcattgatta cattaacctt agttggttgt agtaacggag
     1261 aagataaaaa agtggttgaa ggttctaaga aaattcaaaa ttctaaaaaa gatgaacagc
     1321 ctataatagt acataaagat gcaaatatat tgaaagatag gtatgagcca ccaaaaggat
     1381 ttaaaagagt tgatgtagaa aaaggtagtt ttgaatcatt tcttaggaat actaagctaa
     1441 aaaattatgg agagaaagta aagtattatg atggaagaac taaggaatca aaaggtattt
     1501 atgatagtgt atttgatgtt gatattggca atagagattt acatcaatgt gcagatgcga
     1561 ttatgcttat gagagcagaa tacttgtatc agaataaact atatgatgaa ataagttttg
     1621 attttgttga tggttttaag gcgaagtatt caaaatgggc acaaggatat agaatttcag
     1681 taaaagatag tggttcaagc tggtataaag caactgaata ttcaacatct tatgagagtt
     1741 ttagaaagtt tatggatata gtgtttgcat attcaggaac gttatctctt gaaaaagaat
     1801 tggagcctgt aaaagttgag gatatgaaaa taggagatgt gtttatagtt ggtggaagtc
     1861 caggacatgc aagtataatt atggatatgg cagaaaatga aaagactggt gaaaaggtat
     1921 ttatgatagc ccaaagctat atgcctgcac aacaaacgca acttcttatt aatcgtaaaa
     1981 atccaaattt gagtccttgg tatagtttag attttgaagg tgatttgaca atacctcaat
     2041 ggacttttaa aagagaccag ttgaaaagat tttaaaataa taattttata taataaaaga
     2101 aaaatataga tgtgaaaaaa tgtaattaga ttaaaactaa ttggatatgg acaaaattaa
     2161 attgatagat aaatattaaa aattacacta attaatctta ctatagttta atatttatct
     2221 aaaaagtttt atgtaaaata taaattttat attaaagcta aaactaattt gaggaggcta
     2281 atttatattg aagattagaa ggtatttaat tttggtaatt ataattctaa ttacaacaag
     2341 tcagagtttt atttcttatg ggctagagcg atttgaagaa ataaccattt ttcacactaa
     2401 tgatatacat ggaagatatg ctgaaggtga tgaccatata caaataggaa atctagccac
     2461 attgaaaaaa gaaactccaa actcaatttt agtagatgca ggagattgtt tgcatggact
     2521 tccaatagta aatatggata gagggaaaag tgcaattgaa ttaattaaat gtgctggata
     2581 tgactatatt actcctggaa atcatgactt taattatgga aaagatagac tgtttgagtt
     2641 atctaaaagt gctaatttag gagagaatac tttaaaattt ttatcatcaa atatttttga
     2701 aaatggaaaa aaagtttttg atagtaatga tataaaagaa atagatggaa taaaaatagg
     2761 attttttgga ctctcaaccc aggaaacaaa gagcaaaact ggatttaaaa atgtggaagg
     2821 attagagttt aaaaatccta tagaaagtgc aacagaacaa gtaaatgaat taaaaggaaa
     2881 gggtgccaat gtaatagttg caatatgtca tattggtaca aatagtttaa gtaagccaaa
     2941 tagtatagat atagcaaatg aggttcaagg aattgatttg attattgatg gacacagcca
     3001 cactaagttt gaaaatggta aacaagtggg tgatacattg attgtaagtg caggacaata
     3061 tttagaaaat attggcgaga taaaactaac tttggatact aatgatgtag aaaatataaa
     3121 aatagaaaat aaatcagtta gattaattgg aaaagaagaa gcattaaagt ataagcttga
     3181 ctccgctgta agtaaaaagg ttgatgaaat aaaagaacaa caagaaaaaa tattagataa
     3241 agttataggg gtaactacaa atactctaga tggttcatat gaaaatgtaa ggacaaaaga
     3301 aactaatttg ggtaatttaa taagtgacat tttactagat aaaactaagg ctgatatttc
     3361 tttatttaat ggagggaata ttcgagatac gatagaaaaa ggcaatataa caagaagaga
     3421 tattgtagat gtatttcctt ttaacaatac tatagttacc aaagaactta caggagcaca
     3481 gattaaagat gtattggagc atggagtaaa actttaccca gaaaaaagtt cagccttttt
     3541 gcaagtggga ggaatttcat attactttaa tcctaaacaa aaagaaggcg aaaaaataac
     3601 aaatattcaa aaagaaggaa agccattaga tttaaataaa aagtatgtag ttgcaacgaa
     3661 tgattatata gcatcaggtg gagatgaatt tccttgtttt tcaaaagagc cagtactaaa
     3721 ggaatttgga aatttagaga gtgcagtcat tgaatatata gagtacaaaa cagaaatatc
     3781 aaaaaatgta gatggaagaa ttggtattaa atctaaagat gagttaataa gcaagaatca
     3841 tgagagtgat ttgatattaa gtgaaaatga aaaaaatatc aatgataatg aagagaatct
     3901 atctaataat ataaaaagct ctgatagtga taaaaataaa agcttagata aaacaagtgc
     3961 tagagaaagt tctaatctag gaatatctaa aagagaagct aaagaaaaat ctcctaaaac
     4021 aggagattta ggtttttcaa atagtataat tatatttata gtttcaggta cattgatatg
     4081 tttattgaat tttaatcaaa aagagttaaa ggataaaaag tcaaaataaa aattaatgag
     4141 caaaaactag tagatagtaa tttaatttta gataatagta aacagcaaag gaaattagtt
     4201 atataaatac taatttcctt tgttttatgt tttaacatga ttacttttaa ataaaattgt
     4261 attttatgaa a
//
LOCUS       DAGSHT010000258         4254 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.264, whole genome shotgun sequence.
ACCESSION   DAGSHT010000258 DAGSHT010000000
VERSION     DAGSHT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.264"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1855
                     /locus_tag="MJB72_003404"
     CDS             <1..1855
                     /locus_tag="MJB72_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8264465.1"
                     /translation="KDTEVKAKDTEIKILGTGDLHSILTDSMVSYVNEEREKNKNLLM
                     VDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEYDDIIKSSKDEVPIVKDMAKLKYDA
                     VVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSANIYEQSGENYVQPYVMKNIKTDEGN
                     VKVGILGLTIKEVGETLGLEDFEKDKKARELGEQADYKGKLYANDLVEDAKKWIKVME
                     KESPDIIVAVVHSGEEPKTDRNPGNRIKELATTVDGIDAIVAGHTHEKIDEHKYKNKS
                     GDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKEKSSKLKIIDKEVSIVATADLHSHF
                     SDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEMTQWYKEWKSIRDNNSDKTISRCPM
                     VMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLDAVIEDFEQVAISVLSANIYRQSGK
                     NYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGLKNVNLQEQFQYKDKLYANDLVEDA
                     KKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNRIKELATTVDGIDAIVASHTHEKID
                     EHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKEKGSWTINDKFSKTIEVE"
     gene            2256..4160
                     /locus_tag="MJB72_003405"
     CDS             2256..4160
                     /locus_tag="MJB72_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY8264466.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         1800 a          424 c          830 g         1200 t
ORIGIN      
        1 aaaagataca gaagttaaag caaaagatac agaaattaaa atattaggga caggtgattt
       61 acattcaatt ttgacagact ctatggtttc gtatgtaaat gaggaaaggg aaaagaataa
      121 aaatctgctt atggtagatg cgggagattt ctatggtaca caaagtagag aaatgtggga
      181 atggtcttct ggaaaaaagt taattaatat taaaagaaat ggtagagcag agtatgatga
      241 cataattaag agtagtaaag atgaagtacc tatagttaaa gatatggcaa agttgaaata
      301 tgatgctgta gttttgggag ataatgaatt tgtttcaaat gataagcaaa gtcttgataa
      361 actggtttct gattttaaaa ataataatat gccattggta tcagcaaata tatatgaaca
      421 atctggagaa aattatgtac aaccatatgt aatgaaaaat attaagacag atgagggcaa
      481 tgtaaaagtt ggaatactag gtcttactat aaaagaagta ggggaaaccc ttggtcttga
      541 ggattttgaa aaggataaaa aagctagaga gcttggagaa caagctgatt acaaaggaaa
      601 attatatgca aatgatttag tagaagatgc aaaaaaatgg ataaaagtta tggaaaaaga
      661 aagtccagac ataatagttg cagttgtaca ctcaggggaa gaacctaaaa cagatagaaa
      721 tccaggaaat agaataaaag agttagcaac tacagtggat ggaatagatg caattgtagc
      781 tggtcatact catgaaaaaa tagatgaaca caaatataaa aataaatctg gagatgaagt
      841 aattgttaca caaccaggtt ggcatggaga tagagtatca gagattaatt tcaaactaaa
      901 taaagagaat ggaaagtggg acataaagga aaagtctagt aaattaaaaa taattgataa
      961 agaagtaagt atagttgcta ctgcagattt acattcacat ttctctgata aattaacagt
     1021 tgaccttgtt aatgaacgaa gtaacccaat tgaacctgtt gtagtagatg caggcgactt
     1081 cttagaccct caaacagatg aaatgactca atggtataaa gagtggaaaa gtataagaga
     1141 taataatagt gataaaacga ttagtagatg cccaatggtc atggatatga atcagggaat
     1201 gtatgatgca gtagtacttg gaaatcatga atttgtatct gaaaaagatg aattttggtc
     1261 agatgaacgc cttttagatg ctgttataga ggattttgaa caggtggcaa tttctgtgtt
     1321 atcggcaaat atatataggc aatctggtaa aaattatgtt aaaccatata ttataaaaga
     1381 tgtggagacg agtgaaggta atgtaaaagt aggaatattg ggacttacta taaaagaagt
     1441 aggtaaaggt cttaaaaatg taaacttaca agagcaattt caatataagg ataaattgta
     1501 tgcaaatgat ttagtggaag atgcaaagaa atgggtaaaa gttatgaaaa agaaaaaacc
     1561 agatataata gttgcagttg tacattcagg agaagaacca aaaaatccaa aacatccagg
     1621 aaatagaata aaagaactag caactacagt agatggtatt gatgcaattg ttgctagtca
     1681 tactcatgaa aaaatagatg agcatgaata taaaaataag tctggagaaa aagttatagt
     1741 gacacaacca ggtgagcatg gtaaatatta ttctaaaatt acctttaaag taaataaaga
     1801 aaagggttcg tggactatta atgataaatt cagtaaaaca attgaggttg aataacttta
     1861 taaaatttat agataaagta aattatctga tattggcatg atatataaga taaagtttct
     1921 aatattagta taatgcataa tgtgaaatat tttaataata agagaataaa caagacaaat
     1981 aaaaatattt taacatgtaa gatatagtat gaactattat actaatgctt actttataat
     2041 ttgaacatgt tagaataagt cttatttgaa aattttagtg tactagagta agtattattt
     2101 tactagtaaa aaatattgag aactatatgt agatttgctt aaaaaggaat ttacatatag
     2161 ttcttttcgt ataataaata gtacaatctt atatggtaaa gataaagtgt gatattaaat
     2221 tttattatca tccaaaatct aaggaggagt ctcttatgaa aataaaattt attatagtag
     2281 catcaataat atttttaata gtattacttg tggggtgtca aagtactaaa gacacagaaa
     2341 tcaatgtact cgccacaagt gatttacatt caattattcc agataattta atttcctatg
     2401 tagaagagga gagaaaatat gatgagaatc tacttatggt agatgcagga gatttttttg
     2461 acatgcaaag tggtgagatg aataagtggt ttactggtca aaaattagta agatttaaag
     2521 atgggatacc agagtacaaa aaaatagctg aaccacgtga aggagaagtt ccattggcta
     2581 aaaaaatggc aaagctgaag tatgatgcag ttacatttgg gaatcatgaa tttgtatcta
     2641 atgacaagca gagccttgat aaattggttt ctgattttaa aaataacaat ataccattag
     2701 tatcagcaaa cgtatatgaa caatctggag aaaactacgt aaagccatat gtaatgaaaa
     2761 atataaatac tgacaaggga actgtaaagg ttggtatact tggtctgact ataaaagaag
     2821 taggggaacg ttcaaggttt aaaagttttg aacaagataa aaaggctaga gaacttgggg
     2881 aacaacctca atacaaaggg aaattatatg caaatgattt agtagaagat gcaaataagt
     2941 gggtaaatgt tatggaaaaa aaagaaaagc cagatataat agttgccatt gtacattcag
     3001 gtgaagagcc aaaaaatcct aaaaatccag gaaatagaat aaaagaacta gcaacaactg
     3061 tagagggaat agatgctatt gtagcaggtc atacacatga aaaaatagaa cagcatacat
     3121 ataaaaataa ttctggtgaa gaagtaatag taacacagcc tggagctcat ggtaatagta
     3181 tatcaaagat taattttaag ttggataaga agaatgataa gtggtttata aaagacaaat
     3241 atagtgaatt aaaggttttt ggaaaggaag taaatataat tacaacttct ggtctgaatc
     3301 caaaattttc agatgaaatg gcagttaatt tgtttaatga aagagataga gatgttcaac
     3361 ctatctttat agatactgga gattttttaa atactaatac aaaagaaatg actaagtggg
     3421 ctaaagagtg gcaatacaat gaaaataaag gcatctataa aaaaataaat gaatggccaa
     3481 ttgtatatct tggttatgat gctgtagtac ttggtagcca tgaatttaca gaggataaag
     3541 agacttttga ggaaaaaaaa tctacattgg attatattat agaaaacttt gaagaaatga
     3601 agataccagt attatcagct aatatatatg atgaatctgg agaaaattat gtaaaacctt
     3661 atataattca taatgtagaa actagggaag gaaatataaa ggttggagta ttaggtctta
     3721 ctactactga taaggatgaa gaagacttaa gtaactcaag cttaagcaaa gactcaaaga
     3781 cttcgaaaat tcctgaagaa acaggaaagt tatatactaa taatctagta aaagatgcaa
     3841 gcgaatgggt aaaagttatg caaaaggaaa gtcctgatgt gattgttgca gttgtacatt
     3901 cagataataa tagtactcta aaaaatccaa gtagtgaagt caaaaaactt gcaatgtcag
     3961 tagatggaat agatgctatt gttgtaggtc atacgaaaaa taaggtagaa gaacatattt
     4021 acaaaaataa gtctggggat gaggttatag taacacaatc tggagaagat tgttattcaa
     4081 aaataagtct tgaactaaga aaagaaaatg gcaagtggaa tatcataaac aaatatagta
     4141 aagcaataaa aatgaagtga atttattgat atataaacca ttattaacac tgatttttat
     4201 aatataataa atactgattc tacaatataa ttaacactga tttttataat ataa
//
LOCUS       DAGSHT010000259         4216 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.265, whole genome shotgun sequence.
ACCESSION   DAGSHT010000259 DAGSHT010000000
VERSION     DAGSHT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.265"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(135..656)
                     /locus_tag="MJB72_003406"
                     /pseudo
     CDS             complement(135..656)
                     /locus_tag="MJB72_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            1012..1809
                     /locus_tag="MJB72_003407"
     CDS             1012..1809
                     /locus_tag="MJB72_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBY8264467.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            1919..2509
                     /locus_tag="MJB72_003408"
     CDS             1919..2509
                     /locus_tag="MJB72_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBY8264468.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            2575..>4216
                     /locus_tag="MJB72_003409"
     CDS             2575..>4216
                     /locus_tag="MJB72_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBY8264469.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEAD"
BASE COUNT         1684 a          453 c          732 g         1347 t
ORIGIN      
        1 gatgcatcct gctttgtatg aaatccaata tatctttatt atttaaacaa tggaaaagag
       61 tcttatttat attttgagac atctctttta tttaattatg aagttttata cagttcaata
      121 aataggaatt atatttagaa actcatgaca ttatttctta gatagtgatt aaatttatca
      181 tattccattt tagataactt ttctgggtaa gacattacaa tcaggttatg cccattaaat
      241 tttttatggt atggtggatg aatgtgttca taattatttg tcttatattt tactctttca
      301 tagaacgcct tgcgataaat tgaaatctca tcaattggaa ggtcaatttc taaaataata
      361 gtttttcctt tcttatttaa aagctctaac gctctttgcc catatctatt attgcgaatt
      421 tctggaaaaa tgcagaaatg ttctacatag ataaagttat ctgtttccca acataaaatt
      481 atgccgacaa actggttttt atcataaatt aaattgaatt aatattcttt atttttcaaa
      541 atttctgctt gcaaagaaga ttttctttgt ttatgaaatg ggaaactaat tttatataat
      601 tccatagcct tgtaatataa tttattttca ctggttctta accgttcaaa tttcacttta
      661 ctcctcctaa tcacgaaaat tccaatttat ttatgagttc attttaccat agtcagtatt
      721 tgcatacaat gtgttttaag ttgagtgtca tttctttctt ttatttatgc ttatatctca
      781 tagtctaaga gataattgct taagacataa ctaaaaacta ttatattaag ctttgataat
      841 ctaaaaacag tatatttatg gacttttcta ggtctaaaag taatcttaat aattttagaa
      901 gttacaaaaa agttacataa atttggaaag ctataatata catatataat ataaggttaa
      961 aactgtaatg aataatatta atttaaaatt tgaacatgga aggagattat tatggagaaa
     1021 agagaaaaga ggataataat atcatcttta ttaagtgtat caattttaat gggtttggca
     1081 agtatatatt ctatattaaa taaagaagac ataagtttaa ccgttaaagg tcaagagcaa
     1141 aaagtatctt cctttaagaa aacagttgag gaacttttgg atgaacaggg tgtaaagtat
     1201 aattctgaag ataagattaa tccaagttta gatacagagt taaaagatga tatgaaaata
     1261 aaagttgtta aggtaactaa aagtaaaaaa gaagagattg aaaaaattcc atttgataca
     1321 aagcatgtaa atgatagtaa tttgttaaaa ggaaaatcta aagtttatca agaaggtcaa
     1381 gaaggagaaa aaaaactagt ctataattta acttaccatg atggcaagtt agtcaaaaaa
     1441 gtcttatcaa aagaagtaat atctaaggag ccaactacaa aaattataaa atatggaact
     1501 aaagaaaaag tactaatagc atcaagagga gcaaatataa gaggaggcaa acatatgaaa
     1561 gtggttgcca ctgcatatgc aggagataca ataacatcta ctggtacaac tccaagatgg
     1621 ggtgttattg cagttgaccc acgtgtaata ccatatggaa caaaagtata tatacctaaa
     1681 ctgggtatga cttttgtagc agaagattgt ggaggtgcaa ttaaaggtaa tagaatagac
     1741 atttttatga acagtgaagg aaaagcttca aattggggaa ggaaaagtat agatatatat
     1801 ctacattaaa ttagtgaata gaataacttt acttgaagga caaaaaagta taaaatatga
     1861 attaactaaa ctgttagatt attcttaaaa cataaaaata tatctaaggg gggataagat
     1921 gaaaatagtc tgcttaggag acagtttaac gtatggattt ggagtatcac gaagtaattc
     1981 atggacaaac attgtaaata aagagactcg attagaaata gtaaataagg gtataaatgg
     2041 tgatacaaca agtggtatgt tagctaggtt taatgaagat gttgtaaaaa actctcctga
     2101 tatagttttt ataatgggtg gcaccaatga ttttattgct ggtgcgggaa atgaaattat
     2161 taattctaat ataatggcta tggtgcatca gtcatattca aaaaatatta tacctataat
     2221 aggtatacca ttaaaaccag atattccaaa tgttagggaa gattggagtt gttttactga
     2281 ttttaatata gtatctcaaa acttagaatc atatagttat tggataaaaa aattttgtat
     2341 gacatttaat actaatttcg tagattttta ttcagaatat aataaaaata tggaactaga
     2401 aggatataaa aaattatatt ttgatgggtt acatccaacc aaagagggtc atagaataat
     2461 ggcagacatt tttattaatt caattaataa gtatatagaa gagttataga ttacattaaa
     2521 atgcttgttt atgcgtcagt tgcaataaat acatacagta gagaggggtt tattatggat
     2581 tatactaaaa cttatgaaga gtggataaag ggttcatatt ttgatgaaga tacaaagcta
     2641 gagttggaga atataaaaaa taatgaaaaa gaaatagagg atagattcta taaggattta
     2701 gaatttggga ctgcgggtct tagaggaata attgaagcag gaacgaatag aataaataag
     2761 tacacagtta gaagagctac ttttggattg gcaaactata tattagaaaa cacaacaaaa
     2821 gaagaaacaa gtagaggagt agtaatagct catgataata gacacaagtc tagacaattc
     2881 tgtatagaat ctgcaaatac tttggcagca tgtggtataa aagcatatat atttgatagt
     2941 ttgagaacta ctccagaatt atcttttgct gttagaagct taaatgcaat cgctggtata
     3001 gtcataacag caagccataa tcctccagaa tataatggat ataaggtata ctgggaagat
     3061 ggagcacaag ttatgccaga gattgcaaat gcaataacag agaaagtaaa tagtatacat
     3121 gattatagta caataccaac cttaacggaa gctaataaaa atttggtagt tttgttggac
     3181 gaatcacaag atactaaatt tatagaagca gtaaaaagcc aaattataag aaaagattta
     3241 gttaaaaatg taggtaaaag ctttaagata gtttatacac ctctttgtgg aacagggaat
     3301 gttccaataa gaagagcatt gaaagaagtt ggttttgaaa atataattgt agttccagaa
     3361 gaagaaaatc cagacccaaa ttttgcaggg cttgattatc caaatccaga agagaaaaaa
     3421 gctttaaata gaggtatatt actcgcaaaa gaaaaaggag cagaccttgt aatagcaact
     3481 gacccagatt gtgatagagt aggtgtagca gtaaaaacta ctacaggtga gtatgcttta
     3541 cttacaggaa atcaaattgg tggtatgctg acacattata taatagaagg attaaaagaa
     3601 aataataaat taaaagaaaa tcctacaata ataaaaacca tagttacatc agaatttgga
     3661 gcagatatag ctaaagcaaa taatgttgat gtattaaatg ttcttacagg ttttaaattt
     3721 attggtgaaa aaataaaatt atttgaacaa aataaaaata gaagttatgt atttggatat
     3781 gaagaaagtt atggttactt agttgggact catgctagag ataaagatgg tgtagtttct
     3841 tcattgttaa tatctgaaat ggcagcattt tactattcaa aaggtatgag tttatatgat
     3901 ggcttaatag aactttataa aaagtatgga tttttcaaag aacagactat atctttgact
     3961 ttaaaaggta tagaaggtgt agaaaagata aaagaaataa tatcttattt tagagaaaat
     4021 caaattgatt atataaatag tattaaagta gttgataaaa aagattataa aaatggtata
     4081 gataatcttc ctaaatctga tgttttaaaa tactttttgg aagatgaatc ttgggtagct
     4141 ataagaccat ctggaacaga accaaaactt aaattttaca tagctgtaaa aggagctagt
     4201 gatattgaag cagata
//
LOCUS       DAGSHT010000260         4149 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.266, whole genome shotgun sequence.
ACCESSION   DAGSHT010000260 DAGSHT010000000
VERSION     DAGSHT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.266"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            150..596
                     /gene="rpiB"
                     /locus_tag="MJB72_003410"
     CDS             150..596
                     /gene="rpiB"
                     /locus_tag="MJB72_003410"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBY8264470.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            626..1291
                     /locus_tag="MJB72_003411"
     CDS             626..1291
                     /locus_tag="MJB72_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBY8264471.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            1401..2903
                     /locus_tag="MJB72_003412"
     CDS             1401..2903
                     /locus_tag="MJB72_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBY8264472.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(3215..>4149)
                     /locus_tag="MJB72_003413"
     CDS             complement(3215..>4149)
                     /locus_tag="MJB72_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264473.1"
                     /translation="IYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKN
                     IENGNLKYKLKVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLK
                     TPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFN
                     ITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNIL
                     KYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLG
                     LSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         1622 a          449 c          695 g         1383 t
ORIGIN      
        1 tgatttgaca taagtcaatt aaagctgtct taaaaataga aagaaatttc tacaatggga
       61 cagctttatt tttacaatac tttattttta tgataattat aaaatagtct gaaagtaatc
      121 aaaaaaatta taaaaagagg tgctatttaa tgaaaatatc tattggatgt gaccatggtg
      181 gttatgaatt aaaggaatat ataaaggaaa atttaataaa taaaggtata gaagttgaag
      241 atgtgggtac aaattcaaaa gaaagtgtag attttccaat atatgcaaaa aaggtaagtc
      301 aaaatgttca aagtaaaaaa tctgatttag gcatactttg ttgtggaaca ggaataggta
      361 tgtctattgc agcaaataag tttaaaggaa tcagagcagc tgttgtttct gactgttttt
      421 cagctaaggc aactagagaa cacaataata caaatgtact ttgtttaggg gaaagagtta
      481 ttgggaaagg attagcaatg agaattgttg aagaatggtt aaattcaagt tataatggag
      541 aaagacatgc aagaagatta caaatgattg atgatataga aaaagaaaat ttataaatat
      601 ataatttata acaaggggga gcgttatgtc tattatttgt gcttcaataa tgtgtgcaga
      661 ccaaatgaag ttaaaagagg aattagaagc tcttgaagaa gcaggtgtaa aattacttca
      721 ttgtgatgtt atggatggaa tatttgttaa aaacttggct atgggaccag agttattaaa
      781 atcaataagt gaaaatacaa caataccttt agacatacac ttagccacag aaactccaga
      841 taaatatatt gatatgatgt cttatataaa gccaaaatat atttcttttc atgtagaatc
      901 aagtacaaat gtaaaagctg atatacaaaa gttaagaaat tatggtatag ggccagtgtt
      961 agcaataagc cctcaaacaa gtgtggataa aatagaagaa tatataagtt tagttgaggg
     1021 tatcttagta atgacagtaa atccagggtt tgcaggtcaa aagtttaatc tttctgtatt
     1081 ggataagttg gataaactta cagagatact aaaagattat gataatcctc catttataga
     1141 ggttgatggg aatataaata aggatactat taaacttatg aatggtaaaa aagtagatat
     1201 atatgttgtt ggtacttcag ctttatttaa tgataagcct ccaatttcat ataaagataa
     1261 aatagaagaa ctaaaagaaa gtataaagta accatgtaat aaaattataa tatgtttctg
     1321 tttaatagta aaaatttagg tggaaacaag aaattttata atatttaata aaaattttgg
     1381 ttgagtaagg agaatttttt atgggagacc tatataaact acaaaatggt actgatatta
     1441 gaggaatagc gtatgaaaat gattctaaag aagttaacct aactgtagaa gaagtaaaaa
     1501 aaatagctaa agcgtttcat atttggttaa aagaaaagac taagaaagat aaagtaactg
     1561 ttgccatagg aactgattct aggataacag gaagccaatt tagaagtact gttattgaaa
     1621 ctttaactaa tgacgattgt aatgtaattg attgtgagat tgccacaact ccagctatgt
     1681 ttatgacaac tattatggat ggatacaatt ctgatggggc gattatgatt acagcaagtc
     1741 acttacctta ttactataat ggtttaaagt ttttcacaga aagtggaggt ttagaaaaaa
     1801 ctgatataaa agagatgttg gatatagctg ttaaaaatga tagtgtagac tatgaggaag
     1861 taaataaaaa aggtgaagtt atagtaaaaa atttaataga agattattct aatttattga
     1921 tagataaaat tagaaaaggt gtaaattcaa gtaaaaatta tgaaaagcct ttttctgggc
     1981 ttaagatatt ggttgatgca ggaaatgggg ctggtggatt ctttgctgaa aaagttttac
     2041 acatactggg agcagatacg acaggtagtc agtttttgaa cccagatggt atgttcccaa
     2101 atcatatacc taatcccgaa aacaaagaag caatggaaag catatgtaaa gctgtgttgg
     2161 ataataagag cgatctagga ataatctttg atacagatgt agacagagct gctattgtag
     2221 gtaaaaatgg caaaccaata aataaaaatg ctcttatagc tgttatatca tcaatagtat
     2281 tagaagaaca tccaaaaaca gctatagtaa cagattcaat tacttctgaa ggacttgcta
     2341 aatttataaa tgaattacaa ggaagacatc atagatttaa aagaggatat aaaaatgtaa
     2401 taaatgaagc tataagattg aatagtgaag gtgaagagtg ccatttagct atagaaacgt
     2461 ctggacatgc tgctttaaaa gaaaattatt tcttagatga tggggcatat ttaatagcta
     2521 aaattttgat aaaagtagca aagctatcct tggaaggaaa gactatagaa gaattgatag
     2581 aaaaccttga agaagctcaa gaagagaaag aagttagaat aggcattaat aaagaagatt
     2641 ttagaccata tgcagaggat atattaaaag atttagattc tcatgttaag agtattgatg
     2701 gatggtctgt agcaccaaat aattttgaag gcataagagt taattgtgat gaatcaaatg
     2761 gagatggatg gtttttactt agaatatcac tacatgaacc attattagct ttaaatattg
     2821 aatcaaataa aattggtgga gcagaacaaa tttattctaa gttaaaactt ttcttagaaa
     2881 aatatgattt aaaaggtctt tagggtaaat agaaagaata tatttatatt gaagaataaa
     2941 tgtaaatttg tacctaagat tacaaatatt acaaaattgt aatcttaggt aacagagatg
     3001 taactgatta tggaaacact atgtgatatt atagatacag agagatgaaa tatatgatga
     3061 attatagaga aatataattt aaaatgtatt aatatcacac caaaaaggta taatcaaaaa
     3121 atatgtaaat ataaacccca acatttagaa aaagacctct aaggaggttt ttttcttgtt
     3181 taaataactt aaaatatatt tttatattaa tatatcaatt aactcttcta aaagtaagca
     3241 ttaccttaaa taaatcagca tctataatta tttccatgtg tgcattttct aattcaatta
     3301 tattttgagc aattgataaa ccaagaccag agccatctgt ttttctagat atatcacccc
     3361 ttctaaatct ttctattaac tcttctggct ttagatttag tttatcattt gaaatatttt
     3421 taaacgttaa tattatattt tcttccttta taaacatatc tatgtagacc ctagtgttag
     3481 gcatagagta ttttagtata ttgcaaatta gattctcaaa aactctccaa gtttttttac
     3541 catctatcat ggtaaaaaca gcatattcag gtgtttctat tataaaatct aaactgctat
     3601 tttgaatttt gtcttcaaac tctacaatac tttgaattaa aagttctctt atatcggttt
     3661 ttgttatatt aaattctaca tctccactac ttagcttaga tgcttcaaat atgtcatcta
     3721 ttaataattt taatctttgt gattttttat ctaaaatatt tatatagtca tttatagtct
     3781 ctctgtcaaa gtcattattt tttaacatat caacatagct taatatagat gtaagtggag
     3841 tttttaagtc atgagatacg tttgtgataa gctctgcctt catcctttca ttttttatat
     3901 tagcatctac agcagtttca agaccgcctg ttatattatt tatacttttg gctaaagtag
     3961 atatttcatc atctcctata acttttaatt tatactttaa attgccattt tctatatttt
     4021 ttatattttt tgatatgtta tttatgtaat taaacttaac agataaaaag ttgtatatat
     4081 aaaaatgaaa aactgtgttt acaagtgtaa ataaaattac tatagctatt gcctcagcat
     4141 aatatatat
//
LOCUS       DAGSHT010000261         4110 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.267, whole genome shotgun sequence.
ACCESSION   DAGSHT010000261 DAGSHT010000000
VERSION     DAGSHT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.267"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            5..1555
                     /locus_tag="MJB72_003414"
     CDS             5..1555
                     /locus_tag="MJB72_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8264474.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            1581..>4110
                     /locus_tag="MJB72_003415"
     CDS             1581..>4110
                     /locus_tag="MJB72_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264475.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELL"
BASE COUNT         1589 a          382 c          720 g         1419 t
ORIGIN      
        1 aattatgagg tacaaaaaaa gaaatataga taagtcaagt gaactttcat ttcaatctct
       61 agcaaaatat tttataactt tctatatagt aattatatta attattcttt ctatgttttg
      121 tataaataca atatgggtta tgaaaaaaga taatcaagat ttattatcta caacatcaaa
      181 tcgtattgaa gatagggtag gagaaacaat taagctattg aactctttga gtaaagataa
      241 ttacataata gataatagtg tgaatggcat agataaaagt aaagaacttt caatgtatag
      301 tgatgaattt gggtatatga tgatatgttt tgttgataag aatataattg tatgggatga
      361 agaaggtggt aatattagtc tagctggtcg tgattatatg cagaaacttt ttgcaacaaa
      421 aaaatatcaa gttacagata gttttgcagc aggggctgat ggaaaaactt taaactacac
      481 tattgcatca cctataataa agaatggaaa tgtagatggt tgtgtatttg cagcaattta
      541 ctttgatgag ataaaggata tactcaaaca aggaaaaggt caacagcaac agaatttcat
      601 attatttgga agtgaaaatc aaataatgaa tttaacaagt gatgaaactg catatggaga
      661 gaagtatatg aagttatcgc agaaaacaaa atatcttgga accaatgcaa aaaaagtaga
      721 aaaggatatg tttaataata aatcaggtga ttattgggct tttgaaaatg gaaaattata
      781 ctttatgtcc tataaaccaa ttaagaacac aaattggagt ttattttgta aagttaattt
      841 cttagatatt tttgcattaa atttaattcc tatgggagct attctatctg ttataacttt
      901 agtatttatt attttgattt gtatggagaa aagcttcatg aaaaaacgaa tggaagttat
      961 tgatatgctt ttaggttctg ttcaagaatt agaaaaacgt atatatcaaa cagaaagacc
     1021 tagtgaagta gactttaaag aaattattca gctcacaagt aaaggtctga ctgatggatt
     1081 aactggtgtt gtaacacgta ctgtattttt tgaccaaatt tataatcgtg ttgaaatgga
     1141 ggaaaatgat aaattatctg taatttcatt tgttgattta gatgatttga aatgtattaa
     1201 cgacttatat ggacatgaaa ttggggatat ggttttgaaa aatgttggat atgtacttcg
     1261 tgaatatgag ataaaatata atggtttaat tgggcgctat ggtggagatg aatttgttat
     1321 gttgttatct ggttttacat cgagagaaga ggtggaggat ttattggatg agattgctat
     1381 tcgcttacat actgaagtac taacagatga tggtatcatt caaactcatt gtagtgttgg
     1441 agctgtagta tgtagtgtaa aagacaataa agatatagat aatttattaa aacaggctga
     1501 tgaagcttta tactatgtta aacgtaatgg aaaaggtaat tatcatttag tttaatgtat
     1561 gtggaaggag tttatatcat atgaaaaatg tatctaaaaa attttttgta tgtactatta
     1621 ttgtattaat aacattttta tgttttagta aatttgctat ttatgttttt gacaaacaag
     1681 aaaagaaaga aacaaaagta gtagaaaaca tgttaaatgt ctacaagaat aacattattt
     1741 taaatctgac tgaaaaattt aattatagca aagacttgac atcagtttta gtttcaaaca
     1801 attattctat agataaatta aaacctattg cagatgaaat tcttaaaaac aattcttgca
     1861 tatatatttc agttgtagaa aaatataatg tagctgcatt tttttctaat tctgtcaaaa
     1921 aagatattat caatactcca atacaggaac tgaattattc ttattcatta gcaaattcta
     1981 tcaaaggatt agtcgtagaa ggtccttcta aagaccagtt ttcaaatgga gatgtgtttt
     2041 taattactaa tcctatttat aaaaatggag agtactgggg agaaattaca gtagctttag
     2101 atgccaaaaa agttgtagat tcttttaact taaataaatt aaaagaatct ggatttgagt
     2161 atgaactttg gcgtgtacac ctagataatg gttcaaaatc tacaatagaa atttctacta
     2221 aagattttaa tttttccaaa tcacatgaaa ttaagtttaa acttccgact gtttggactc
     2281 ttagcattgt gtacaaaaat ggatggaata ataagaatat agattttatt gtatttgtta
     2341 tattgttaat tatttccata ttaataggtg tgatgacatt tatttacatt gaaaagaagt
     2401 cgattagtaa ggaattaaat caacagagtc ttaaaaattt agaaactggt attttgaata
     2461 gagaaggttt tttaattaag ttacaagagc atctgaatag aaaaaataat gaaatagtgt
     2521 tgctgtatat atgtattact aattatagta aatttgctcc agttatgagc tttgaaaaaa
     2581 gacaagatta tcttacacat atagtaaaaa atctagataa ttacataaaa agttctcatt
     2641 ttattgctcg tataagtgag gaaggttttg ttctagcttt agaagaaaat tgtgataaaa
     2701 atgaattgct taacattgaa aaagggattt ccttagagct tttacgaaaa attaaagtag
     2761 aaaataaaca agtattttta acgattggtt atggctcatc taggtgtgtt gatgatagaa
     2821 aagatgcaga atctctgtta ctttcagcta ttgaagatta tgagagaaga tttccagcaa
     2881 tgtctcctat tcatgatttt actaaaaagt gtgaagatat gcttgatgaa aagaaacaga
     2941 ttgtatttgg tgaatatcca gatttagaaa taaacaagtt gtctaaagta atatacaaat
     3001 attataaaaa agtacagcgt attatgtata aagattataa tattgatatt ggaaatagac
     3061 gagggtatat gcgtgatgtc gaagtattaa ttgattataa ttctgagaga atgttatact
     3121 tatttttaat agacatatgc aagtttggaa aatttaatga actgttttct attgaaatag
     3181 gagatgttat tttaaaagaa gttagtaaca aattaaaaac aattttttct aattatctat
     3241 atcgtattaa tggagatgta tttttaggaa tttcaataga taaagaatct tacttagata
     3301 ctttacataa ggttaatgaa gtatttaata gttctttaag tgtagaagat gtactttttg
     3361 atgtaaaatg taatattggt atttgtcaat atccatatca tggtgaaaat ccatatgacc
     3421 ttctggaaaa ggtacaaatt gcattggagt atgcaaaaaa ggatgtcaaa agtaatgtta
     3481 aagtttatga taaggaattg acagaagaga tttaccatga aaataatatt attaaggatt
     3541 taaaaaaagg tctggaagaa caaacactag aagtttggta tcagccaatt tttaatgtta
     3601 gtaaaaactg ttatataaaa gctgaggcac taattagatt gagaaataaa gagggcggtt
     3661 attattctgc ctatgaagtt attacttatg cagaaaagca tggacttatt aaggatgtag
     3721 gtctatatgt actaaaaaag gcttgtgatg taactacaaa aataaaagat aaaaatatag
     3781 attttgaaca aatgcaatta aatttatctg ttcagcagat tgtacataag cagtgtgcaa
     3841 atgacatatt atcatgtatt gagaattgtg gtttagataa aaagtatatt tgtgttgaag
     3901 ttacagaaac agtacttatg gaaacttttg aaattgtaaa tgttactctt gataaactaa
     3961 aaaaagaagg cattagcata gctttagatg agtttggttc tggatttagt agtataaatt
     4021 atctggcaaa tttacctatt gaaaatttaa aaatagataa ggaattagta gaacaaatta
     4081 cctattctaa aaaacaattt gaattattaa
//
LOCUS       DAGSHT010000262         4088 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.268, whole genome shotgun sequence.
ACCESSION   DAGSHT010000262 DAGSHT010000000
VERSION     DAGSHT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.268"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            299..1132
                     /locus_tag="MJB72_003416"
     CDS             299..1132
                     /locus_tag="MJB72_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBY8264476.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1225..2157
                     /locus_tag="MJB72_003417"
     CDS             1225..2157
                     /locus_tag="MJB72_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY8264477.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KINLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2209..2691
                     /locus_tag="MJB72_003418"
     CDS             2209..2691
                     /locus_tag="MJB72_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8264478.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2703..3572
                     /locus_tag="MJB72_003419"
     CDS             2703..3572
                     /locus_tag="MJB72_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY8264479.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3615..>4088
                     /locus_tag="MJB72_003420"
     CDS             3615..>4088
                     /locus_tag="MJB72_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HBY8264480.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSSNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNL"
BASE COUNT         1811 a          380 c          683 g         1214 t
ORIGIN      
        1 tatttattat ttgtttaata tttctaattt ataaaaaaaa aattataaat tttaaaaatt
       61 ataaatgaca ttttaactag aattaattgt aaaaaacact atataatact caattttcaa
      121 cattctatac aattatacag aaattataca gcataataat caccgaaata acaaatttta
      181 aaaattaagc aaaacttttt gtcgaagttc aacgtctata tatatgtagt gtaaattttt
      241 gggataaaat actacaaaaa tataaatgta aaaatatagt aatattggag gtcgaacgat
      301 gagaaataaa aagaaaaaaa taaatagaaa gaagttgtat ttgcttttaa gtgctattgt
      361 agtgtgtctg gcttttatag tagttggtgt tcgcgtcagc tctttaaata tgaaggaaaa
      421 tgaaattagt agaaatgcaa aactgtcaag ttctactatt acagatatag tatctaatac
      481 atcaatagaa gttagtaaag ggccaagtaa aagttctgga aaaatagcat acataactat
      541 agatgatggt ccatctaaat ttactgacga gatgataaaa actttaaata aatacaatgt
      601 taaagcaact ttttttatga tagatgggaa tatgaaggaa tatccacagc aagttaaaaa
      661 tataattaaa aatgggaaca cagcgggttt tcatagtgta tcacatgata ttcacaagct
      721 ttatgttact agtacatcag caaaagagga atttgataca aatgatcaaa ctttttataa
      781 gataactggt aaacattcaa aagtaataag aataccttat ggaagcaaac catatacacc
      841 acaagcctct tatcaggctt tagttgatgc tggatataaa atttgggact gggacttaga
      901 tacagaagac tggagatcta attcttctca aattgtacaa aatgtaaaaa atcatattaa
      961 gaatagaaaa ggtgaagata aagaccaact tgtagtttta atgcatgaga aaaaacagag
     1021 tgcagaagct ctagattcag ttttaaaatt cttatcagat gaaggttatg aatttgcgcc
     1081 agtagatcaa aaccaaatac caaaaaatta ttggttacgt aatttagagt agttaaaata
     1141 taagaatatt gattgatatt tttagaaatt gagaataaaa atgagtgagg tgtaggtata
     1201 ttgaataaaa aactttatat aggaatggtc ggaatattgt ctttgatgat ggttggttgt
     1261 aataaaagtt tagcaaaagt aaatgatgtg gaaatcacaa aagaacagta caaaaaaaca
     1321 aaagcagttt tatctgctac taataattat ataaatgggc agtctttaga tgaattagaa
     1381 aaaactttgg ataaaaaagg tagaaataaa ttagaaaatg ttataatatc gtttatggtg
     1441 gataatgaac ttttatatca agaggctaag gataaaggac taactccaag taaaagtgaa
     1501 gtagactcaa aatatcaaga actagaagat aagataaatt tgaatacaag ctataaggaa
     1561 aaaatggaca aagcaggtgt tgataaagat tatttaaaac aagaaatatc tagagattta
     1621 gcaatagata aaaataaaaa ggcctttgaa gatagaataa acataagtga taatgatatg
     1681 gaagcctact atacaagtca taaaaaagat tttaatgtag aagaagtaag tgcttctcag
     1741 atacttatat cgactttaga taaaaataaa aaagaagtaa gcaaagataa aaaagaagct
     1801 ttgaagaaga aggcagataa tatattgact aaaatcaaaa atggagaaag ttttgaaagt
     1861 ttggctaaaa aatattcgga tgataaagca actggaaaaa atggaggaca gctaggatat
     1921 tttacaaagg atgataaaaa tgctgagttt acaaaggaag tgttcaaatt aaagaaaaat
     1981 gaagtttcaa atgtgtttga aactagttat ggatatcata tagtcaaagt tactgataaa
     2041 agagaaagac aaaaaagttt taatgagtgt cagagtttaa taagagaatc tattttaaat
     2101 gaaaagtata ttgagcatat aaaaaaactg aatgaagatg ctaaaataga cagatagaat
     2161 ttctaacagc tcaatcctag cctgtaatcg gagggagatt ttacaggaat gtttaagagt
     2221 ttaaagagaa cgaaagttga gaaatacatt atcgataata aagactcttt ttatagaata
     2281 gcatatagtt atacgaagaa tgaagaagat gccttagatg tggttcaaga agccatgtat
     2341 aaggcactat actcagtgga aaatattaaa gaagtaaatt atataaagac atggttttat
     2401 aaaattttag ttagaacgag tatagatttt attagaaaaa accgtaaata taataatatg
     2461 acagatattg accttataga tgagactgga gagtatgata aatatactga tttggattta
     2521 agaagagctt tggaagaatt acctattgaa tacaaatcta ttataatatt aagatttttt
     2581 gaagatttaa agattgaaga agtagcaata atattggatg aaaatgtgaa tactgttaaa
     2641 actaggttat atactgcttt aaaaaaacta aaattaaaaa ttgaagagta gggataaaaa
     2701 gaatgacaag caaagaaagg ttaaataagt taaaagatga gtatcataaa attccaatac
     2761 ccaagaaatt agatactata ataaatcatg aaaaaataga aaacatatat cgagagaaaa
     2821 ataaaaaagt aaatagatta agatttaaag ttgcaatagc ctttgcatgt atatttacag
     2881 tattagtaaa tataagtcct gtatttgcag acaatttttc taagatacct gtaattggag
     2941 caatagtaga agtaataact ataaaaaatt atagtttaaa aagtgaaaat tatgaagctg
     3001 agattgatat tcctaaaata cgaggtttaa aagataaaaa cttagaacaa agattaaaca
     3061 gtagttttat ggaagatgga aaaaggcttt atcatcaatt ccaagagaga atggagaaga
     3121 tacaatcatc aaagaataaa ggatataaat ctttaagttt gagttatagt gtaaaaaata
     3181 attctaaaaa atttctatca atagagatga caaaaaatga gatagaggct tcatcatatg
     3241 tgagtaaggt acattatact attgataaaa aaagacaaat agttttgact ttgccaatgc
     3301 tttttaaaga tgataagtat ataaaagtta taagtgataa tataaaagaa caaatgagag
     3361 aacaaatgaa aaaggatagt acaaaatcct attttataga tcaaaaaaaa gatttgcctg
     3421 ttgaagattt taaaacaatt aataagtatc aggattttta ttttaataaa aatgaagacc
     3481 ttgtaatttg ttttgatgaa tatgaagtag cacctggtta tatgggcgca gttgagtttg
     3541 taataccata taaagtaata aaagatttgt aaattgaata gtagtttata tataaatctt
     3601 ttaaggagga caatatgact acatataaaa aacttaaaaa atctatattt aaaattgcta
     3661 tattttcaat atgcttatta gtttcattag tagatacaaa ccctatattt gcaggtagtt
     3721 cctctaatga gacttcaaat agtgatacga taaaggtgtt aaatgctaag agtatcaact
     3781 ttaaacaaga caattatgaa gtgaatgtga gtattcctaa aataaaaggt ttaaaagata
     3841 aaactttaga gaataaatta aacaatgagc ttttgcaaga tgggaaaaaa atttacagtg
     3901 aatttcaaaa taaaatgaaa aaggaaaata ataagggtca tagataccta aatgtaatcc
     3961 ataatgtcaa aaataattct aaggactttt tatcaataga aatgacaaaa gaagagatag
     4021 aagcttcctc atctataact aagaagcatt acactgttga taaaaataga ggaatactat
     4081 taaattta
//
LOCUS       DAGSHT010000263         3957 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.269, whole genome shotgun sequence.
ACCESSION   DAGSHT010000263 DAGSHT010000000
VERSION     DAGSHT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.269"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(168..944)
                     /locus_tag="MJB72_003421"
     CDS             complement(168..944)
                     /locus_tag="MJB72_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBY8264481.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(1001..1972)
                     /gene="asrC"
                     /locus_tag="MJB72_003422"
     CDS             complement(1001..1972)
                     /gene="asrC"
                     /locus_tag="MJB72_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBY8264482.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(1985..2776)
                     /gene="asrB"
                     /locus_tag="MJB72_003423"
     CDS             complement(1985..2776)
                     /gene="asrB"
                     /locus_tag="MJB72_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBY8264483.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(2777..3784)
                     /gene="asrA"
                     /locus_tag="MJB72_003424"
     CDS             complement(2777..3784)
                     /gene="asrA"
                     /locus_tag="MJB72_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBY8264484.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         1254 a          778 c          364 g         1561 t
ORIGIN      
        1 aactatataa ttcatatata aacgtatttt ttatatatat aaccgatata ttttcgtatg
       61 ctcccatatt ttttttgttt caaatatttt ttcaaaaata taaaaataac tctctcaaca
      121 atactaaaaa tgtatttatc aagagagtta taatttatac aataaattta attaagcttt
      181 tctttaccta atatataagt tccaagaccc ataaataaag ctcctccaac aatatttcca
      241 agtgttacag ctactaaatt ataaatagct cctccaatag taactgtact tattgttggt
      301 gaaaataatg aaacactata aattgtcata tttgctacac tatgctcaaa acctgatgtt
      361 ataaaagcaa ataaacataa gaatatcata attatttttg cagtatcact attagttcta
      421 aacgaacaca acactgatac acatactaga atattacata aaatccctct aaagaaaaga
      481 gctgtaaatg gcatggatgc tttactagct gctgtatttg caaaaaattc agctacaggt
      541 cctttgtcta ctagaccagt tcctacaaat ataatgccta aaaccaaagc tccgattaaa
      601 ttacctaccc aactgtatgc ccatatttta gaagtatctt ttatacttac acctttattt
      661 aacataccag cagacataac catattgttt ccagtaaata actctgttcc cgtcattata
      721 accaaactta gtgctattgc aaatgataac cccattacta tcttagtcat aggtgaacct
      781 gcatcagtca aaagtcctcc aatagtaaat atcaaaagta tacctatacc tacataaagt
      841 cctgcaaacg cagaagatac caaatatttt actttacttg tgtttaataa atttatctta
      901 tttatagctg cattagttaa tttatccaaa gtttctttat gcataactcc ctcttccttc
      961 tattaaattt taatttattc aaatatatgt tttactcaat ttatagatat ttaattccac
     1021 tccagtatat gttttcgtac attttagtta tttctggaag ctctacacct tcaagtgccc
     1081 attttttaaa ttccataaat cctgttctat caattatata tcctatatgt tcttttcctc
     1141 ctggtgcatc tttatctata tacttttcta catatctata agtattaagt attatcttta
     1201 ttatgctgtc ctcatctacc cataaaagaa aatcttctgc tagtctaggg tttttctttc
     1261 ctgttctacc cattatagct aatctatagt attttttctc atctctagtc catgcatttg
     1321 ttgggcaatt tagtacacac tctccacagc ctatacactt atctttatct cttaccacct
     1381 tattattttc tgtttttaat gctccaacag atatcttaga acattttttt acacatgctc
     1441 cacaatttac acatctatcc ttttcatata ttggtaaagc cataccaata attccaaagt
     1501 cattcattct agcttttata caatcatttg gacaaccagt taaagccact ttaaaatgta
     1561 aatcatttgg aaatatagct ttttctattc ttttagcaaa ttcagttgta ttatattgag
     1621 cctttggaca gactttattt cctatacaag ctgctatatt tcttgtacct gctgctggat
     1681 aacctgcgtt tttctcactt tgatttatat tcattttttc aataacaggt tgtataattt
     1741 tatttatttc ttccatatct tccatattta ttcctaatat ttcaaacccc tgtcttgtag
     1801 ttatgtgcac ctgtccattg ccgtactcac ttgcaatttt agaaactaca gctaaactct
     1861 cagggtcaat aagacctcca ggtactctaa ctcttaaaga agtctcatat tttgtttttg
     1921 taattctata agcatttttc attacttttt tggtatttaa atctcttatc acaaacttca
     1981 ccccctaatc aataagattt ttagcatatg aataatcaaa tactggtcca tctaaacata
     2041 tgtaagtatc atccatttta caatgtccac atttacctat tccacaacac atttttcttt
     2101 cataagaaac ccaaatgttc ttttcatcta aatctctttt taaaaattct tctactgtaa
     2161 atttcatcat cattggaggt ccaactacaa ctatagaagt attgtttata tctttcatat
     2221 ccaattctgg tatgtatttt gttactaatc ctacattacc actatatcct tcctctgctc
     2281 catctacagt aactaaaata tcaagttttt tactccatct ctttaaatca tctgcaaata
     2341 aaatatcttt tggtgattta aatcctacta ttaatttaaa cttttcgcat ttttctttat
     2401 tattataaaa atattcaact attcctctta caggggctaa tgcacttcct cctgccacta
     2461 ctacaatttc tcttccttca taaagggata catcaaatcc atttccataa ggacctctta
     2521 taaaaaaaga atctccttct ttataattaa acaactcgct agttactttt cctacatttc
     2581 ttattgtaaa gtcaatataa tcctctccat atcctgatac tgatattgga ctttcaccgt
     2641 atttaggtat agatatctca taaaactttc caggcaatac accttttgta ttgcagttta
     2701 ctctaaaagt ccattcttta tcagtatgtt taactatatc tagtattttt gcacttactg
     2761 gtatatatgg gttcatttat acttcctcct tactaattac taaatcattt attttttcta
     2821 tacactttga aaatgatatg tattgcggac aggcatcatc acatcttcca caaccaacac
     2881 acatatgata tccgaatctt tttttaaaat catgtatttt atgcatagtt ttaaatctca
     2941 ttctttcacc ttgttttttt ctaaaagaat ttccacctgc tatatctgta tatccatcta
     3001 cttgacaaga agcccaaacc cttcttctct cacctacatt ttcgttttcc ttatagaaaa
     3061 tatcctgcat tgtaaaacat gaacatgttg gacatacaaa attacatcta ccacatccta
     3121 tacatcttgt atcatattca tcccatattg aatttcctat catttcattt gaatcaatat
     3181 tctctggcac atctacagat attaaattat cagttacata atcaacatta aattcagttg
     3241 cttctccatt aaatacttca aattcactat cctttatatc taagtacagt gtatctcctt
     3301 ctatatttaa tccaattgaa taattatcag ttttgttaga atccatgctc acacaaaaac
     3361 agtttctaaa actttcctta caccctacaa gtgcaaactt tactttatct ctgatatttt
     3421 tataaaaata atctttttct tcaccattat ttagatatat ttcatcaagt ctttttatac
     3481 catttatgtc acaagctctt aagaaaatta aaattttctt gttgtctaaa tcgctttcct
     3541 taaactcttt ttcagtaaaa taaaacaata cttgatttat gggaagtaca acttcttttg
     3601 gtgaaaagtt tgctttctta gcaaattcca tttcttcaaa agcgtttacc tccttgtatc
     3661 ttataacatc agtatctgaa aaagtacctt taaatgggat taaaactggt gcaaatatct
     3721 tgtagtcttt ttttagataa ttcagacctt cattgaactt agatgaatct aattttagtt
     3781 tcatttttat cacctttctt atattttagt tttacttact tgctatcttg tttaaatctt
     3841 aacactttat tttctatgct tccgtgataa ttatcacagt ttcaaaaata ataaaaaaaa
     3901 tattaatgga gtttattaag ttctgttaat atttttttat tatctacatt attcaat
//
LOCUS       DAGSHT010000264         3954 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.270, whole genome shotgun sequence.
ACCESSION   DAGSHT010000264 DAGSHT010000000
VERSION     DAGSHT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.270"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            55..1458
                     /locus_tag="MJB72_003425"
     CDS             55..1458
                     /locus_tag="MJB72_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY8264485.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1549..1789
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1832..2530
                     /locus_tag="MJB72_003426"
     CDS             1832..2530
                     /locus_tag="MJB72_003426"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBY8264486.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2553..3554
                     /gene="trpS"
                     /locus_tag="MJB72_003427"
     CDS             2553..3554
                     /gene="trpS"
                     /locus_tag="MJB72_003427"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBY8264487.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1478 a          448 c          721 g         1307 t
ORIGIN      
        1 ttaattttga aattaaattt aaaataatta atagagaggg gaaattaaat taggatggaa
       61 aagaaaaaaa taggcctatg gagtttggta tttatgaatg tatcagcttt atatggtata
      121 agatggatag ctaaatctac agcatcaagc tttggtttag gattaggtgc aatacctatg
      181 tggttattat tctcatttat atactttgta ccaggagctt taatttgtgc tgaattagct
      241 tctacatatc aaaaaggtga tggaggtctt tacgattggg ttaaggaagc ctttggagaa
      301 aagtatggat ttttagtttc ttggttaaac tggacagcta aaatattttg gtattcttca
      361 tttttaactt tcttagcagt aaatgtttct tatacaatag gaaatccagc ccttgctgat
      421 aataagatgt ttgtacttat attatcttta ataatattct gggcattatc tcttatagca
      481 actaaaggaa taagttttac aaaaatattt actaatacag gtgcattagg ttcaacaata
      541 ccaagtgcta tacttataat attttcattt gtagcagtat ttgtgttgaa aaaacatgat
      601 atagcttcta catacacaat agcttcaatg actccaaaac ttaatatgga tgcatttgtt
      661 gctatatcag ctataatgtt tggtttagca ggagcagaga ctgctgctaa cttcataaca
      721 gaaatagata agcctgaaaa gaacttccct aaggcaatat taatatcagc tggaatagta
      781 gcttcattat atgtattagg ttcaattgca ataacaatga taataccacc agaccaaatt
      841 actgcttcta aaggtgtatt agatgcactt tctgctgttt gtgcgtcttt aggaattggt
      901 tcttggttgg ttcaattaat agcttttggt atagcttttt cagtattagg agcaatagtt
      961 ctttacatag catcaccaat aaaaatgtta tttggtagtg ttaaaaaagg aatatttcca
     1021 gatagtttag tagaggtaaa tgaacacaag ataccttcta aagcagttat acttcaagca
     1081 ataatagtta ctataatact attggtgact acattaatgc cttctgttga tgctatatac
     1141 aatgttcttg taactatgac agcattaact gcattattcc catatgtatt gttatatgct
     1201 tcttatatta aactaagaaa agagagacca gatgaaatta gaccttatac tatggcaaaa
     1261 tcaacaagta cttgcctagg tttagcaaaa atggttttag ttgtaactgt agtgggtata
     1321 ttattatctg cagcaccagt tatgcctaca tttgctgaaa atgttatata tgagatagag
     1381 atgattggtg gaggtcttct agttatatta tctggtctat ggttatggaa tagatatgaa
     1441 aagaaaacag ctaaataaaa tgtattttaa aaatttcttt tggatattat aatatatagt
     1501 tgacaattta tagtgtaagg aatattataa aataaagaaa ttaataaatt aaatcctgag
     1561 ataagaaata gtaaataaat atatttttgc acagagaatt aaggatgctg agaacttaat
     1621 aaaaagtatt tattgaatgg gtctttgagg agtaaaatga aatgtaattt aatattacag
     1681 agtatttgat atcgtgtatg cacgttatag catatattaa gatatatata ttcaattttg
     1741 agtgtatata aatttaggtg gtaccacgga aaatatatcc gtcctattga tttaggacgg
     1801 attttttaat atagaaaatt taggaggaaa aatgaacgta ataggaatta taggtgcaat
     1861 ggatgaagaa gttagtattt tagttgattt aatggatatt agagagacta taaaaaaagc
     1921 tagcttagaa ttttataagg gaattttgga aggtaagaat gtagtgttag taaagtgtgg
     1981 cataggtaag gttaactctg ctctttgtgc ccagatacta ataagcgaat ttaaggttga
     2041 tgctattgtt aatacaggtg ttgcaggagc tttaaacgaa aaacttgatg ttaatgatat
     2101 agtaatatca actgatgcta tacagtatga tgtagacact acagcatttg gagaccctaa
     2161 gggagttata ccaagaatga agacttctgt atttaaagca gatgaaagac ttatagatgc
     2221 tgcttacaaa tcttctgtgg aagaagtaaa gactcataaa gtattaaaag gaagagtagt
     2281 tacaggagat aaatttataa attctaaaga attaaaagaa gaattagtaa atgattttgg
     2341 tggatattgt ggagaaatgg aaggtggagc tatagctcat gtatgctatt taaataatac
     2401 tccatttgta ataataagag caatgtctga taaggcagat ggaagtgctg atgttactta
     2461 tgatgtattt gtacatgatg ctgccaataa ctctaaagac atagttttaa atatgttaaa
     2521 atcaatataa atatataagg aggtacaaaa aaatgagtga aaaaaaagtt atatttagtg
     2581 gtgcacagcc atcaggcaaa atgactttag gaaattattt aggagctata aaaaactgga
     2641 ctgaacttca agacaattat gattgttatt atagtatagt tgatttgcat gcaataacag
     2701 tacctcaaga ccctaaagtt ttaagagcca atactataga attacttgct caatatatag
     2761 cttgtggatt agacccagaa aaaaatacta tatttataca atcacatgtc aaagagcatg
     2821 ttgaacttat gtgggtattg aatactatga cttatatggg tgaattaagt agaatgactc
     2881 aattcaaaga taaatcacaa aagagtgaag caaatctaaa tgcaggttta ttcacatatc
     2941 cagttcttat ggctgctgac atattgttgt atcaaactga cctagttcca gttggagatg
     3001 accaaaaaca acatttagaa ttagctcgtg atttagcaaa tagatttaat aatagatttt
     3061 cacctacttt tgtagttcca gaaggatact acccaaaagg tggagcaaga gtgatgagtt
     3121 tgcaagagcc aactaaaaaa atgagtaaat ccgattcaaa tgaaaatgca tttatactat
     3181 tagcagatga ttcagattct ataaagagaa agataaaaag aagtgttact gattctttag
     3241 gtgttgtaaa atacaatgat gaacaaccag gtattaaaaa tctaattgat atatattcta
     3301 atttaagtaa aaagagtgta gaagagattg taaacatgta tgaaggaaag ggttatggca
     3361 tatttaaaga agatgtggct gaagtagtat cagaagcttt aagacctata agagaaaaat
     3421 atgttgacct tcttaataat aaagattatt tagaaaaaat atatagtatg ggagcagaaa
     3481 aagccgaaaa acaagctaga aaaactctta gaaaagttta taaaaaagtt ggatttatag
     3541 agagaagata ctaattatta gctatcttta cttgataaat aaattttaaa gttattaaaa
     3601 aggtatatga tatgaaagag agagtctcaa agtgaacttt tgttcatgag gcactctttt
     3661 ttgtatgaaa taaaatatat tttaatcatt tcaacaatga aaaagaggat tatctctatt
     3721 ttgagacagc atcttttgat aataaaaaaa gatgagcttt tgctcatctc aaaggggtta
     3781 gattattctt actttgggga aagtagaata aaggtaaaag ttaactgatg tcaactttgt
     3841 cttttttgca tatcactact gagcataaaa aaatcagaaa accatagcct tctgaaacaa
     3901 cttctaacat acattgtatt ttatacccaa tatgtaattt atgagttaat ttta
//
LOCUS       DAGSHT010000265         3935 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.271, whole genome shotgun sequence.
ACCESSION   DAGSHT010000265 DAGSHT010000000
VERSION     DAGSHT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.271"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     repeat_region   8..430
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8..36
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1261..2133)
                     /locus_tag="MJB72_003428"
     CDS             complement(1261..2133)
                     /locus_tag="MJB72_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY8264488.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(2527..3105)
                     /locus_tag="MJB72_003429"
     CDS             complement(2527..3105)
                     /locus_tag="MJB72_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY8264489.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3163..3879)
                     /locus_tag="MJB72_003430"
     CDS             complement(3163..3879)
                     /locus_tag="MJB72_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBY8264490.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
BASE COUNT         1250 a          671 c          362 g         1652 t
ORIGIN      
        1 caagtatatt tacattccat atagttaata taaaacaatt gataagcaaa gtcaaaaata
       61 aacattcctt ttggtattta cattccatat agttaatata aaacggattt taaacctcct
      121 tttgatgttc ctgctagtag atttacattc catatagtta atataaaacg gagattctgg
      181 tgttattaat attcagattc cttatcattt acattccata tagttaatat aaaacttaga
      241 tttttagagt gcaacatgaa aaaaggtctt taatttacat tccatatagt taatataaaa
      301 ctatttagac aagtaaaaaa ccactctgcg caaaagagat ttacattcca tatagttaat
      361 ataaaaccct actaaacacg ctttagaggg aaacaagtta gatttacatt ccatatagtt
      421 aatataaaac cccaaaataa attaagtatt tccaatactt atacatatac aattctccta
      481 aacttgcagt gagcgagcaa tagtgcaatt gataacagtt atcatacact ctcaacgctt
      541 tatatttcaa ctattaaacc ctactttatc acaaaaatcg ctcactgcaa aacttttata
      601 tttttattat atcataaaac tcatactata aaataaaaat gggataattt tacaaactaa
      661 aagcatctac attcttataa gtatttatta tattctctat tccaatgatt cctttttctt
      721 tttattgcat ccttcctatg taatcgcacc ttagctcaat aagtctttta ttttcattaa
      781 caatttttta aattattcaa aatcatcaaa tttttcatct tatacagtta aatcaaaata
      841 cgtgttatat ctctttatct atatactttt cttccttcca tttcttttcc tctttataca
      901 ttttcacttt gttactttac aatctcaagg ttattatctg ctattaaagt tttatctttc
      961 caggtttcac aaaaataaat tgaataagtg caaaaataac agatacaaaa ccataactat
     1021 tttcttttct ataaaaaaat atataatcaa caagaaatat aataaatgaa aataactatg
     1081 aataaatact ctcatttaga agaaatagac aaagatattc cttaaaattt aagcaaaata
     1141 ttgtttaaat aaaaaaactg actgtcagtt aaatgccagt cagctatttt atatatttta
     1201 tccaaacatc actatatttg aacattttaa taattaataa tacttattaa tatacaaact
     1261 ttactcatac acagaatatc ttttccaaat ttgctctaaa tcatcaaaat gctgtttaat
     1321 atcttctttt tccttcatcc caacagatat agcaagggca tttgaaatac taagagctgg
     1381 aactagagaa tctacaaatg aagccatatt actcttaact agaagagtgt tatctgcaag
     1441 tgatgctact ggcgcaaata agctatctgt taatgatatt acatgggctc ctttttcctt
     1501 agcataattg acaatttgat aagatttctt agaataacga gggaaactaa tagcaactat
     1561 tacatcttct tcatttattc ttacaatctg ctcaaaagca tctcccatat ccattctaat
     1621 tatatgaaca ttgtcaagta ttatatctaa gtaaaaacct aaatattgag ctactacaaa
     1681 agaactcctc attccaagta tatatatttt tctcgctctt aaaagtttgt tagaagcttc
     1741 tttaaacgct ctctcgtcaa tttcttctag tgtagacctt atattatcta tatcactttt
     1801 taaaactttg tttagtattg caaaatcatc ggaataatca tgtgccattt caactctttg
     1861 tactgtagtt agtttatttt ttatgagttc ttgaagtgca gcttgtaatt taggatatcc
     1921 actataacct aatgcatttg caaatcttac aactgtagac tcacttactc ctacggtttc
     1981 acccaattta catgcagtca taaatgcaac tttatcataa ttatttaaaa tgtattgtgc
     2041 tataagtttt tgacccttac tcaatctcgt atattgagat tgtatatttg atatcaaatg
     2101 tttactgtcc ttcatttcat ttgtttcttc catttttttc tccttttagt tatataaact
     2161 attttgtttt attaacactg tctttttctc taaaaatttt attttttatc tcaatttcat
     2221 cttagttttt atcatagctt cttaaactca taaaattcta acttttctgt accttatact
     2281 tcacatagta ttttcgatta aatttattat accatctttc cttgcatttt ttaatattta
     2341 tccaatattt cttttaaatt tttcaatttt tactcattta aaagttatct atcttatttt
     2401 gcatatttag atgtatattt tgttttgaga ataagctgtt gtatacttat tatttaaagt
     2461 atttttaata ggtcacacta tatatattaa ctcttaatac aatcttgtca agttttttag
     2521 tatttattat gcttttttct tttcaattaa aatcaattgt ggtgggttat tgacttgatt
     2581 taaaaactta gattctaaaa cattaaattc atcttgattt aaattattga caaggttata
     2641 tatgcaatct ttttcttctt gacctccatc atgccctgta tatatggcaa tgcttattac
     2701 tccattttct tttaacattt ctaaacttaa ctttatagcc tttaaagttg tatctggttt
     2761 ggttataatt gaatgtttcg ctcttggaag atagcccaaa ttaaataaaa cacatgaaac
     2821 ttcttctttt atatattgat tcattttttc atgcccatca aatatcaatt ttactctatc
     2881 tgtatatcct tctttctcta gtttctttct tgtagacttt attgcttctt cttgtatatc
     2941 aaaagcatat acaaagccct tttctccaac cttctcagct agataccttg tatcataccc
     3001 atttcccata gtagcatcaa ttactatgtc accttcattt ataatatttt ctaaataaaa
     3061 tttatttata tctgtaattt tagttaaata ctttgcatat gccataatac tatttctccc
     3121 tactattaac tttatttcat tttttctcaa tttattttta tcttatttta ttgattttaa
     3181 atattttatt tcatcttctg ttaggtatct atattctcca actttggtgt cttttaatac
     3241 tattttcccc acagaaatcc ttcttaaatt taaaactgga tgatttatag ctttgcacat
     3301 ttttcttact tgtctatttc taccctcatg tatagttatt tcacacactg aataattttt
     3361 ttctttattt tctttagtta cccttatttt ggcaggtgcc gttgtgtaat cttctatttt
     3421 tagtcctgtt tcgaattgtc ttatctcatc atcacttata attcccttca cacttgccac
     3481 atacgtttta tcaatttcat gttttggatg ggtaagctta tatgttaaat cgccatcatt
     3541 agtaagtatt aataatccac tagtttcata atctaatctc cctattggat atactctttc
     3601 ccttacatct ttaagtatgt ctattacact gggtctattg aattggtctt ttactgtagt
     3661 tatatatccc tctggtttat ttaatactat atatatataa ttttcacttg gttttacttt
     3721 tttattatca aattctacta tgtctttttc ttcatcaata ttaaaagata actcttttac
     3781 tacacttcca tttaccttta ttcttcctcc aagaattatt tcttctgctt ttcttcttga
     3841 tgctattcca caagaagcta tatacttatt aattctcata cttaatcctt ccttatctta
     3901 aattatgtgt gtttaatatt tttatcaatg ttaat
//
LOCUS       DAGSHT010000266         3901 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.272, whole genome shotgun sequence.
ACCESSION   DAGSHT010000266 DAGSHT010000000
VERSION     DAGSHT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.272"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..664
                     /locus_tag="MJB72_003431"
     CDS             <1..664
                     /locus_tag="MJB72_003431"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HBY8264491.1"
                     /translation="ETRGKGVFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFK
                     DLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKM
                     GCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIK
                     LESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEK
                     VN"
     gene            661..1677
                     /locus_tag="MJB72_003432"
     CDS             661..1677
                     /locus_tag="MJB72_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8264492.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPEID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            1733..1894
                     /locus_tag="MJB72_003433"
     CDS             1733..1894
                     /locus_tag="MJB72_003433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264493.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            2102..>3901
                     /locus_tag="MJB72_003434"
     CDS             2102..>3901
                     /locus_tag="MJB72_003434"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY8264494.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGD"
BASE COUNT         1651 a          344 c          633 g         1273 t
ORIGIN      
        1 tgaaacacga ggaaaaggtg tatttaataa agtaataagc tctgttaatc tgctaaaaga
       61 caatggattc agtaatataa agctgtctat ggtatttgga caaaaaaatt ctcatttaaa
      121 taaacaattt aaagatttaa ataaaaagct tggaacagaa tttgttgaaa gatattttat
      181 tccaacagga agaggcctag aaaataaaga aagctattta attgaagata cagttcttcc
      241 aattacaatt ccgtatattt tagataatga attaaaaatg tgtaaagata aaaaaaataa
      301 aaaaatggga tgttgtgcat gtaatgcagg aaactctcaa atatttataa attatgatgg
      361 agatgtatat ccttgtcctt cactgataaa ggatgactat aaaatgggaa atatatttga
      421 caatgaattt gtaaaagtta taaaagaaaa taaaacaaga gatctaaagt ctttgattaa
      481 attagaaagt atttatccat ataactttga aaaatgtaaa gattgtgatg taaatatttt
      541 ttgttggcat tgcccagcga tattagattc agttaaggat aatgaattgg aattggatag
      601 atggtgtagt atgatgaaac acaatttaaa tacagctgta tggggagatg aaaaagttaa
      661 ttgaaattta ctatatttgt aactgaagac tgcaatttga gatgtacata ttgttatgaa
      721 ggtaatgata aaaaaaaaga aaatatgagt tatgaaactg ctgataaaac aatagatttt
      781 atttgtaata aagaattaga aagtaatagt gattatacaa caattgtttt acatggagga
      841 gaaccattta taaattttga tttgattaaa tatatacata gggatttagt agatagattg
      901 agtgacaaaa agatattatt tgatataact acaaatggaa ctattataaa tgatgaaata
      961 atagagttta tagcaaagaa tgtaaaaaac atatcaataa gtattgatgg aaaaaagaat
     1021 atacatgata taaatagaat agatgctaac ggagtaggta cttatgaaaa agtaatggaa
     1081 aatggaaaaa gacttataga taaaggtata tatgttaggg caagaatgac atataactca
     1141 aatactgtta agtatcttta tgagagtgta tatgatatag caaaacaagg gtttaaaaat
     1201 atcgcaccaa tttcagattt tatggataaa aattgggata attctcatat taaaatatta
     1261 aatgaacaaa ttagtaaaat tatgaatatt aaaaaggatt atcctgagat agatatatct
     1321 attttagata aacaatttat aaataagaaa aaaggagatt gttttggagg gataaatagt
     1381 tatgcaatag atactagagg agatgtatat ccatgtacag tatgtgtagg agataaagat
     1441 attttactag gaaatatata tgatggatta gatgataaaa aaattaagaa ccttataaaa
     1501 atttatatat ctaagaatga agagtgtgat ggatgcacta gacttgatta ttgtatgtca
     1561 accagatgta aacttatcaa taaaaaggta acaggtaaat tccaaagtgc agtaccaatt
     1621 tattgtggat tagaaagagt ttatgtggaa acgacaaaaa aactatgtag tttataatct
     1681 agtttaacta gttataataa atataaaaca aaagtaaagg gggatatgta ttatgataca
     1741 tataaaaaaa atcgaagaaa aaatagaact tcaagatgtt gaggtgcaag cagatccatg
     1801 taaatggtat tatgataagc atggaataat ttcacaaata gactgtatgg tggattgtgc
     1861 aggatcaact ccaaaaggaa cagtagctta ttaaaaaata ctaatcagtc tattaaatat
     1921 gttgaaagta ctagatgtag ttttttacaa atgaaatgtg attttctcaa ctctagtatt
     1981 tttaataatt aaatttaact agcacaacga gttagttagt atttaatgaa tagtgctcta
     2041 taacctacta ttttaggtta tagagttttt cttgtaaata tgaataagga ggattctgat
     2101 tatgataaat attaaacttg cagtgtcata tttaaaaaga caaaaaggaa aaactttatc
     2161 attaatactt agtataggga ttgctgttat gctagttttt tcattaagtg ttattaatga
     2221 atctcaagga caatcagata taaatcatgc atacaaaaac tcgggagatt atcatgcttt
     2281 ttatgaagat ataaatagtg atatagtaaa agaacttaat ggtgaagaag atataaaaga
     2341 attcaatgat gtacttaaat ttggagaaat tataagtaaa gataatggag catctctaaa
     2401 tttatgctct tacaataaag attttttaga ttctactaga tataagttag ttaaaggtag
     2461 agagcctaga acttctaatg aattagtaat agaacaaaaa gttttagaca aaatggaatt
     2521 agatgtcaaa ttaaatcaaa ctattgattt tcagattgtt aatgagcata taaataaaaa
     2581 taatgaaaat tctatttata gtaaagataa gcaatttaaa atagttggta ttttaagtaa
     2641 atctgatgaa tactatgaaa atttaaataa atataaggta agagctttta catattcaaa
     2701 caatggcaaa gatattgttc catatgaatt aataactcat atgggtacat taaaattgaa
     2761 tacaaaagat ttagattctc aaaaagtagt gaatctaggg agaaaatata agttaacaag
     2821 taatcaattt tatccaaata cagaagtaag tatgtctatg tatatgaaaa atatttcaaa
     2881 aaaaacaact tattcaataa agcaaagctt actcccttta ttatgtgcaa cattagtaat
     2941 atacaatatg tttaacataa taatactaga tatgaccaag caaattggtt tacttagagc
     3001 tgttggagct agcaagagaa atattagaca ggtatttttt attcagagta ttgtagtttt
     3061 atctgtaggt atagtaattg gtttacttgg aggtatagtt ttttcttact taggtctcat
     3121 gatagtatat gataaaagtg cagaattaac tataaataca aactctataa ttgaatctat
     3181 catcatgtca attatagcag ttatattagc ctctgtagta ccaatatata aagctggaaa
     3241 tttatctata attgatgcta taaaaaagac agataagtta aattatattc ctaggctttt
     3301 gttgttaaaa tcacataaaa aagttgttgg aattataaat gagatagctt ttaaaaatat
     3361 atttaggaat aaggttacat caataataat aataattacg atatcactat gtggggtttt
     3421 atttataggc agactaactt ctacaaaatt catatatgga aaagagtcag attcagctaa
     3481 tataacaagt aaatcatatg gcaattttga tatttatttg ggatattcac caataaatgc
     3541 aaattataca ttttctaaat atgataattc tttattaaat gaaataaaag ctattgaaga
     3601 tgtaacagat gttgaaccca atatttattt aaaaggatat ctaagaaatg ctgacttaga
     3661 agaagattat ttagatgagc taaaaagaac tgaaataaat aataataatg agtctacagt
     3721 tttaattcga ggttacaata aagagctatt aaataatata gataaatata tagataaagg
     3781 cgaaaatgtt tatagttata cagacaggga ttataaaaat gttttattgg taaataattt
     3841 ttattctaga attaaattgt caaataatac tcagattata aaatcaccta aaataggaga
     3901 c
//
LOCUS       DAGSHT010000267         3856 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.273, whole genome shotgun sequence.
ACCESSION   DAGSHT010000267 DAGSHT010000000
VERSION     DAGSHT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.273"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..905
                     /locus_tag="MJB72_003435"
     CDS             <1..905
                     /locus_tag="MJB72_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264495.1"
                     /translation="NNEKEYEELQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNK
                     LSKELGLDKISSKLEKYEGIIIPSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIF
                     PTGLFSKVVVISDDLYNEVKGEFSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYN
                     RSYFLTLPDMYLVELQQSKILQFMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKL
                     SKIGLSFKNMNKIVSIEIGTLFFIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLL
                     ISSIYSLYFYLLKKIYTRKVWFFD"
     gene            1024..2415
                     /locus_tag="MJB72_003436"
     CDS             1024..2415
                     /locus_tag="MJB72_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8264496.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            2522..3562
                     /locus_tag="MJB72_003437"
     CDS             2522..3562
                     /locus_tag="MJB72_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY8264497.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
BASE COUNT         1556 a          383 c          647 g         1270 t
ORIGIN      
        1 ataataatga aaaagaatat gaagaactac aacaaattag agaagcaatc aaaggatatg
       61 attataagga gttaagtttt cctgtactta aatataatag ggaagtctta ctaagtgtaa
      121 agaattataa caaattatca aaggaattgg gattagataa aattagttca aagttagaaa
      181 aatatgaagg gattataatt cctagtagca attcgcaaga acatataaat tctttaaaac
      241 aattaaaaaa ttatagtata aaaaatttaa atatagaagt aaaaaaatca aaaaataaaa
      301 taatttttcc tacaggactg tttagtaaag ttgtagttat aagtgatgac ttatacaatg
      361 aagtaaaagg tgaattttca aaaattaatt ttcatggatt tgattataaa aactggcaaa
      421 gcagtgcaaa gataactgaa aatttaaaag aaaaacattt taatttggat agagaatata
      481 acaggtcata ttttttaaca ttaccagata tgtatttagt agagttacaa caaagtaaaa
      541 ttcttcaatt tatgggtgtt tttataggag tgatattatt tatagcagtt tttagttttt
      601 tatactttag attatataca gacgagatag aagataatgt aaagtacaaa aaactatcaa
      661 aaataggtct atcttttaaa aatatgaata agattgttag tatagagata ggtacattgt
      721 tttttatacc gtattttata gcaattataa atagcataat ttctttactg tttttaaatc
      781 aagtattaaa taatagtttt aagttagaaa atttgggtat attactactg atatcatcta
      841 tatattcact ttatttttac ttattaaaaa aaatttatac tagaaaagtt tggttttttg
      901 attaaagtgg tatataatag atatataaat taatatttaa aaagtagtta atattagggg
      961 agggcatttg cacctccctt gtaatatgtg gaaaattgaa tattagaaga aggaggagac
     1021 ttgatggaaa atcaacaatt attaggaaca gaacgaatta gtaaattact actaaaatat
     1081 tctataccag ctataatagg tatgttggta aatagtttat ataatgtagt agatagaatt
     1141 tttattggaa atataccagg ggttggacca cttgcaataa caggtcttgg tgtaacaatg
     1201 ccaataatga caataatact tgcatttggg atgttgatag gtataggaac tactactacc
     1261 atatcaataa aacttgggca aggtaaagta gaagaggcaa gaaaacttat aggaaatgca
     1321 atgacgttat ctgtaataac aggaattatc ataatgatac ttggaatatt atttgcaaat
     1381 aaaatactta cattgtttgg agcaagtgaa aatactttaa tatatgcaaa atcatatatc
     1441 aatattatat tattaggtac agtagtaaat cttctttcct tttctttaaa ccactcaata
     1501 agagctgatg gtagtcctaa aatttcagca ggaataatga tagtaggatg tttaactaat
     1561 atagttttag actggatatt aatatttgga ttcaatttgg gaatacaagg agctgcaatt
     1621 gctacagtaa cttctcaggc tcttacagct attttaacta tagggtatta tataagtgga
     1681 aaatcaaact tacgattcag taaatctaat ctaaaattag ataaaaagct aataaaagca
     1741 gtgtttgcaa taggtatgtc gccatttgct atgcaacttg cagcaagttt agttcaagta
     1801 atatcaaata ttgcacttaa aactcatggt ggagatttag ctataggggc tatggctact
     1861 atttcttcta ttgcaatggt atttttgatg cctatctttg gaataaatca aggagcgcaa
     1921 cctattatag gtttcaatta tggagctgaa aaatatgata gggttaaaaa agcatattta
     1981 ggttcattag tagtagctac tataatttta tgtatgggta tggtagtagt tatgcttttc
     2041 ccagaagcta ttataggtat atttaataaa gaccctgaac ttatgaatat atctgttaat
     2101 ggattaagaa tatacttact tatgttgcca attgtaggct tatcagttac aggaacaaac
     2161 tttatacaat ctataggaaa agcaaaaatg gctatgttat tgagcctttt aagacaagta
     2221 attttattga taccagcagt attaattctt cctacattct taggtttaca gggtgtatgg
     2281 acagctcaac cagtgtctga ttttatagct acagtaatta caggtatagt tgtatttaga
     2341 gaattaaaaa gatatactcc taaaactgaa aaattaaatg aaaatgaaag actaaacgag
     2401 ataactactg agtaaaaata taaattcaga taattgagca agaaattgca aaaaatacga
     2461 ttatatgata aaataaaacc gctgtcaagg ataagagaaa ttctaattat ggagtgattt
     2521 tatggaattt acaaagaaaa gcaaattaaa aaatatgtat gtaaacatga atgtagctag
     2581 aatgttgtct aagataaatg agtataaggg tagacaactg ctttacaaaa aacaaccaaa
     2641 agaaatttta gaaaacttag aaaaaaaatc tttagtagat tgttctgaat ctacatacat
     2701 tggaaatcaa agagatagta gtaattttaa tctagaaaag ttgataagta atgaagttac
     2761 acctagaagt agagaggaat tgagtattgt tgaatacaga gatgtggtaa aaactattaa
     2821 cagtgcttat gagagtattc ccatcagttc acaaacgatt cttgagcttc atggatactt
     2881 atataaattt tcatctacta gaggaggaag ttataagtca gataatgatt ttattgaaca
     2941 caatttaaaa cctagcgaat ttataagtat tgattcaagt gtcaataaag ttgaaaaagc
     3001 tgtagaagaa atatgtgaag cttataatac attgatagag gaagatgaaa ttgatatatt
     3061 aatattaatt tctgcatttg tgttagattt catattgata catccattca aagaaggtaa
     3121 tataaaaatg gcgagagtac ttatcctatt gttactaaat aaaaatgggt atgaagttgg
     3181 aagatatata agtttaggaa agatatttga tgatagttca tatgaatatt atagtaattt
     3241 aaattatttg aaagcctcaa taggtagtga aaaagcagat atgaatgcat ggattgagta
     3301 ttttttagag acgatattaa ctgcatatga gaagctggat gatagtttaa atatatctga
     3361 taagaaaaga caaacaaaaa ctagtagaat agagaaaata ataaattcta cccttggata
     3421 ttttacaaaa gaagatataa gagatttatg tcctgacatt ccagaaccta ctataaatag
     3481 agtttttaat aatctaagaa aacaagataa gatagaagtt gttgcaagag gaagaagtgc
     3541 aaaatggaag aaaaaatatt aaaaaatgtt tagataacta aaatggtata gtgttttaca
     3601 ctataccatt tttaatgtca ttagcatgag caataattta acaacttaag caatatcaat
     3661 tttatttatt gattattttt gaactgaaaa atggcgttat atggtatacc tcagtggtaa
     3721 ttaattttgg atgagaagat ataatattac agaatatatt aggagtatat gtagaaaaaa
     3781 tataataaaa gtgtgaggct tagtaattaa caaattatgt ggcttcacac ttttttataa
     3841 aataaagata cttagc
//
LOCUS       DAGSHT010000268         3820 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.274, whole genome shotgun sequence.
ACCESSION   DAGSHT010000268 DAGSHT010000000
VERSION     DAGSHT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.274"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..568
                     /locus_tag="MJB72_003438"
     CDS             <1..568
                     /locus_tag="MJB72_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBY8264498.1"
                     /translation="RFVASDKDLQEVFFIIEGVALVECTTRGGNKFLVDIVPENEFIG
                     KISYIYEHNLKCDIFAKTNIKLFRFEKNVFEEFYLKPDFIALFHRKCTRRIYELYKTR
                     MVRELFSCTEVIAYCISNKQENNVCNIKAIRNFSEIYSISRKSRYHSLLKLTEKEIVK
                     KMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(708..2084)
                     /locus_tag="MJB72_003439"
     CDS             complement(708..2084)
                     /locus_tag="MJB72_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8264499.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2100..>3820)
                     /locus_tag="MJB72_003440"
     CDS             complement(2100..>3820)
                     /locus_tag="MJB72_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY8264500.1"
                     /translation="CEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLRELEVKIS
                     EESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDMDKPISIL
                     SGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVMLISHDRYF
                     LDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQEESIERL
                     KAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQIRDVSMG
                     YGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFGTNVHVSY
                     FHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTLSGGERAR
                     VAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYFLNTVVDK
                     ILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKEREQREIEK
                     KNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLENNLASLYD
                     EWEEFM"
BASE COUNT         1344 a          602 c          469 g         1405 t
ORIGIN      
        1 aagatttgta gcatcagata aagacttaca agaagtgttc tttataatag agggagttgc
       61 attagtggag tgtactacta gaggaggaaa taaatttctt gttgacattg tacctgaaaa
      121 tgaatttata ggcaaaatta gttacatata tgaacataat ttgaaatgtg atatatttgc
      181 aaagactaat ataaaattat ttcgatttga aaaaaatgta tttgaagagt tttatctcaa
      241 accagatttt attgcactat ttcatagaaa atgtacaaga agaatatatg aattatataa
      301 aaccagaatg gtaagagaac tattttcttg cacagaagtt attgcctatt gtatatcaaa
      361 taaacaagaa aataatgtct gcaatataaa agctattcgt aatttcagtg aaatctattc
      421 aattagtaga aaaagtagat accattctct gctaaagtta actgaaaagg agatagtaaa
      481 aaaaatgggt aatacatatg aaattttaaa ctatgatgaa ttacataaat tggcatttga
      541 agtaaaagaa tttttggaag atgagtaaaa attgttactt aatcaaaata aagtataata
      601 atttctgtaa aaaatagaaa tagtctcact gaaaatcaaa aatactttag ttattttcaa
      661 tgagactgtt tagaaatatt gtatacttat aatgtatagt ataattatta atcaatttcc
      721 tttactatat taatagccca ttttttagat aatgctctag gaacaccaaa tataaactta
      781 accacttctt cactataagt gagcaaaacg agtacaaata taggtgcacc tttaaatgca
      841 gctataaaag ttagaggaat agaagctaac cacatacatc ctaattctaa aaatagagca
      901 tatttagtat ctcctccact tcttaaaata ccaattataa ccaatgtatt aaaagattta
      961 agagccatta gtattcccat tataaagaat atcttagtta tatcagaagc taagctatca
     1021 gaaacactaa acatttttaa taataatggt atatttaaaa ttaaaataat tccaagtatt
     1081 aaaccagctg aaaatacaag tatagaaaac tttttagcat attcaatggc tcttttaata
     1141 tggtcagctc caagttcatt tccaagcata atagatgcac cagatgcaat acatacagct
     1201 gtcattatga agaaattacc tgtactggtt gctatctggc tagctgctat agcagaagta
     1261 ccagctcttg catatgcaac agaatacagg acagttccaa ttgcccatac agagtcattt
     1321 acaaatatag gaaagctttt tgatgaaaaa gattttataa aaattttatc aatagctttt
     1381 agatttttta gaccaaactt taatatatag tcttttttat aaaggtaaac ataagtaatc
     1441 attaaaatta attcacatat tctagcaata acagtagcta gagcagcacc ttttacaccc
     1501 aatgcaggaa gaccgaagtt accaaatata aatccataat ttaaaataac atttgttaca
     1561 agtgcaaatg cactacaaat catacctaac ttaggatttc taacacctct tgaaccagtg
     1621 ctaaatacag tacttacagc aattaatgga taactaaata ctgcaatact aaagtaatct
     1681 atacaaagtt ttaccacctc agaatcatac gaaaatatgt gtataattgg ctttggtgtt
     1741 agtaaagcag gaataacaaa tacaaaactt aaaacaattg ccaatacaca agtaagacct
     1801 gtgacttttc ttatattact cacatccttc tttccataaa actgtgaaat aaaaactcca
     1861 gcacctcctg tgataccaga taaggacata ttaaaaagaa agaaaaattg gtttgcaact
     1921 ccaactgaag ctacagatgt ttcccccaaa ctacttatca taattgtatc tattacattt
     1981 acagaagtag aaattaagtt ctgtatgata ataggtatac ataaagatag aagtatttta
     2041 taaaatcttt tattttcgtc ttttaaagaa aatagttctt gcaaataatc ctctcctttt
     2101 tacataaatt cttcccactc atcatataga gaggcaaggt tattttctaa attaattttt
     2161 tcttggctaa cttctttagc tttatctgga ttagagtata cttcttcttg acataaaagt
     2221 atatctaatt tttcaatttc aatctctaac ttttcaattt ctttttctat attttgtctt
     2281 tttattctat ttttcttttc tatttctctt tgttctctct cttttctttt ttcatctttt
     2341 atttgagttc tagttttttc ttctttttct tctataatac tcatctcttg gatttgcctt
     2401 tttttatcta tataataatc ataatttccc agatattcag taattccatt ttcatctaaa
     2461 actaaaattt tatcaacaac agtatttaga aagtatctat catgtgatat tgtaaatata
     2521 gtcccatcat aattagttaa tgcctcttca aggacttcct tagaatcaat atctaggtga
     2581 ttagtaggct catctaataa caataagttt gcatttgata gtataagttt aagtatggca
     2641 actctagctc tttctccacc actcaatgta gaaatcttct taaaaacttc ttcatctaca
     2701 aataaaaatg cccctaacat atttctcaaa gtagtttgag taagatgagt attattttcc
     2761 caaatctcat caattattgt gttatctaaa ttaagagttt tttgttcctg atggaagtag
     2821 gaaacatgta cattagttcc aaatttaata ttgccactaa gaggttgtag ttcattagtt
     2881 attattttaa ataatgtaga tttgccaaca ccatttgcac ctataagtgc aactttttcc
     2941 cctctatata tatctaaatc caagtcttta aaaagtattc tttctccata acccatagat
     3001 acatctctaa tttgaagaac atcattacca ctctggacag aaggcgtaaa ttgtatacga
     3061 gcttttttcc tataagcttc tggtttatct aaaacgtcta ccttgtctaa tgctttttct
     3121 ttacttcttg cacgctttag gtgtttttct ctaccataag cttttagtct ttcaattgat
     3181 tcttcttgct tttttatttc tttttgttgg tcttcaaatt tcttaagctc tagttctttt
     3241 tctattttag ataactcaac aaatttagag tagttaccat tatatacttt aagttttttg
     3301 ttgtgaactt caaaaattct atttacggat tggtctaaga aatatctatc atgagatatt
     3361 aacatgacag tccctttata ttgttttaaa aatacttcta accattctat tgcttcagag
     3421 tctaagtggt tagtaggttc atcaagtaac aatagagttg gtttcttaag aagtaatttt
     3481 cctaaaagaa ctctagtttt ttcaccacca gaaagtatgc ttattggctt atccatatca
     3541 ttgtcactaa atcctaatcc ctttagaact cctttggctt cagatttata tccatatcca
     3601 tttaaatctg aaaataattc tagtttatgt gaatattcat ccatgatttt ttctataatt
     3661 ggagagtttg tttttgtact ttcttcagat attttaacct ccaattctct taaataggat
     3721 tccatttcta tcaaattttt aaaaacttct agtacttctt ccaaaatagt attatccgaa
     3781 taaaaattgg tattttgttc taaatatccg atttcacaat
//
LOCUS       DAGSHT010000269         3763 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.275, whole genome shotgun sequence.
ACCESSION   DAGSHT010000269 DAGSHT010000000
VERSION     DAGSHT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.275"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            581..1483
                     /locus_tag="MJB72_003441"
     CDS             581..1483
                     /locus_tag="MJB72_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264501.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            1470..2225
                     /locus_tag="MJB72_003442"
     CDS             1470..2225
                     /locus_tag="MJB72_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8264502.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVILDVKDKEKEDLTVEEILEKFEYAV"
     gene            2322..3359
                     /locus_tag="MJB72_003443"
     CDS             2322..3359
                     /locus_tag="MJB72_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8264503.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
BASE COUNT         1407 a          486 c          556 g         1314 t
ORIGIN      
        1 ttatctaaat atctctttat ttttataatc ttttatgact tatttatatt tttcattttt
       61 ataaaaaaac aataattaag aatataacta tattctacat gtaaaaattt ttttatttaa
      121 actcaatttt ctgataattt gataattttt caaaatttat agttgacttt tattttttac
      181 tgtattaaac taattactat acaaaacaga aaacaaaatt ttcaaaatta ttaaattaaa
      241 aggagaggta aatatgaaga ctttattaaa caaacaagta cgatttttta gtagccaatt
      301 tagttagggt ttgtacctaa gttaagacaa attgtcataa catagataca agccccttag
      361 gattatgatt tttaaggatt gtatctatgt tggctatagt aattgttgtt aaagttttta
      421 ttttatcatc ataatataga gccacatgga ttatactgtg tggcttattc gaatttttgc
      481 catacagtta tatatactgt gtggtttttt tatatcaaaa tcatttttaa aaccaaattt
      541 aaaaaaccat ttaaaatatt ttcaaagggg tgttttattt atgtcaggta ttatatctgt
      601 aatgacgcaa agtttaattt tatcgattat ggcactgggg gtttatataa cttacaaaat
      661 cttagatttt ccagatatgt ctgctgatgg tagctataca atgggtgctt ctattgtagc
      721 attttctctt actaatggta tttctcctgt agtagcaact ttaatggcta tcttatgtgg
      781 ttgtactgct ggtcttgtta cagggattct tcacattaag tttaagatat ctaatttact
      841 ttctggtatt ttagttatgg gaatgctata ctctatcaat ttaagaataa tgggaaaatc
      901 aaatattcct ctatttagct ttaaacactt attcaatggg gaaatatctc ctatagtttt
      961 agccttagct tttgtattca tctgtaaagt ccttttagat ttattcctaa aaacaggtct
     1021 tggatacact ttaaaaggtg ttggtgataa ctctcaaatg attaaatctc taggtataaa
     1081 tataggttct ataaaaatac taggacttat gatttcaaat ggacttatag ctttatctgg
     1141 tagtcttatg gctcaattct tgggattctc agatgtaaat atgggtatag gaactttagt
     1201 tcttggaatt gcctctatca taattggtat tacactattt aagaaattta cttttatcaa
     1261 agatactact gcaataattg ttggctcttt tatataccaa ttcactatat actttgcaat
     1321 gagtttagga atgttatcaa ctgacttaaa actaataact gccatagtta taatagcttt
     1381 cctagctact ggtaacttaa atatttcact aaaaaaaaac aaatgcaaag ctagtaccaa
     1441 aaataaatca gaaaaaagag gtgttgtaga tgttacaaat taaaaatctt tcaaagagtt
     1501 tccctaatcc atatggtgaa cctaatacta tctttgaaaa tttatcaatc gatatagaag
     1561 atggtgaatt tgtaagtatc ataggtagta atggaactgg taaatctact ttattaaata
     1621 taatatctgg gcttataaaa gaatcttctg gtcaaattac tttagatagt actactataa
     1681 caaatttagc tgagtataaa agaactcaaa tagtaagtag agtttttcaa gacccaagcc
     1741 ttggaacttg tccttctatg acggttagag aaaatttatc tctagcactt aataaaggga
     1801 aattattgaa cttaaaaaaa tgtcttcgtc ataaaacgaa tgacttggaa cacttacttg
     1861 aaggaatttc tttggattta aaaaaatatt tagatattaa agtacaatat ctttcaggag
     1921 gtcaaagaca atcactatca ctaattatgt ctagcttagc aagtcctaag gtcttattac
     1981 tagacgaaca tacagcagca cttgacccaa aaacatcaaa tgaagtaata gagttaacag
     2041 ataaaatcgt aagagaaaaa aatataacta cacttatggt tactcacaac ttgaaacacg
     2101 ctcttcaata tggagatagg ttgataatgc ttcataaggg tgaagttata ttagatgtaa
     2161 aagataaaga aaaagaagat ttaacagttg aagaaatctt agaaaaattt gaatatgcag
     2221 tataagaaat taaaatctta gttttaatca gaaatattta taatgtatca tatgaatata
     2281 tgagcaaata taatcaataa attttaaggg aggaaaataa aatgataaaa agtaaaaaaa
     2341 tattaagttt aattatagca ggagtacttg gtgtatcaat gcttactgga tgttctcaaa
     2401 atgatggctc caatgcttca aatgaaaata aagaaacaga tagcaaaaaa caaaaaaata
     2461 taggtattac tcaattagtg gaacatccat ctctggataa agcaaagaaa ggattcatca
     2521 aagcactcga agataaaggc tataaagatg gagataatat aaaaatagat ttccaaaatg
     2581 cacaaaatga tatgcctact acacaaagta ttgctagtaa gtttgtatcc gacaaaaaag
     2641 atttgatata tgctatatct actccatctg cacaagcagc ttataatgct actaaagata
     2701 ttccgataat aatgactgct gttacagacc ctgtagaagc tggacttgtt aaatctcttg
     2761 aaaaaccagg tggaaatgtt tctggtacat ctgattatct ttcaattgat aaaacattag
     2821 aattggttaa aacattgact ccaaaagcaa agaaaatagg ggttatatac aatactagtg
     2881 aagttaattc aaaaatccaa gttgattctc tacatgatta tgctaagaaa aataattatg
     2941 aagtagttga aaaaggaatc agctcttcaa gtgaagttaa ccaagctatt tctagtttag
     3001 ttggtaaaat agatgtttta tatgtcccta ctgacaattt aatagtttct tctatgccaa
     3061 tagtttctaa agttgctaat gaaaacaaga ttcctataat agcctctgaa gaaggttctg
     3121 tatcttctgg tgctttagct tgttgtggaa tagattatga aaaactaggt tataaggctg
     3181 gagaacttgc tattgaagta ttagaaggaa aatctgttgg cgatatacca gttactacat
     3241 tagatgaaac tgaaataata attaacgaag atacactaaa agcacttgac atgcaaaagt
     3301 tatcagcaga taatataaag tatataaagt cagatgaaaa tgcaaaatct gcaaaataaa
     3361 acttattaat ttcaaaaaat aggtaatatg aagtaggtaa tatgaaaact ttatataata
     3421 agtactctta tccaccctaa ccctttgaaa attgtgctta ttgtagcaat ttgaatattg
     3481 catataaagt tattcatttt ccataaaagt cgtctatatt gacgactgat tatgaaagtt
     3541 atataacttt ctaatcacta cttgagctgt ccaaaagaat tttaatattc tccagacagc
     3601 ttttttttat gatatttgat tgatatacat gtctagttat tccatattta gtaaatattt
     3661 tgataaaatt tccacattca cttataactt aggataacgt ttttttcaaa caatacatta
     3721 atttaaaagt attctgagta tacattattt agtattttaa tta
//
LOCUS       DAGSHT010000270         3744 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.276, whole genome shotgun sequence.
ACCESSION   DAGSHT010000270 DAGSHT010000000
VERSION     DAGSHT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.276"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            16..1248
                     /locus_tag="MJB72_003444"
     CDS             16..1248
                     /locus_tag="MJB72_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBY8264504.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQRLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            1268..2212
                     /locus_tag="MJB72_003445"
     CDS             1268..2212
                     /locus_tag="MJB72_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBY8264505.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            2306..2713
                     /locus_tag="MJB72_003446"
     CDS             2306..2713
                     /locus_tag="MJB72_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBY8264506.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            2800..>3744
                     /locus_tag="MJB72_003447"
     CDS             2800..>3744
                     /locus_tag="MJB72_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBY8264507.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHL"
BASE COUNT         1530 a          342 c          662 g         1210 t
ORIGIN      
        1 aaagaaggtt tgtatatgaa aagattaatg attttaggct caattaagta ttttgagaat
       61 attgttatat cagctaaaaa agagggaata tatacaattg tttgtgataa tagaattgat
      121 actcctgcga aaaagatttg tgatgaagca atagatgttg atgtatttga ttttgataaa
      181 ataaaaagta tagctataga taaaaagata gatggaattt taactggatt tacagattct
      241 ttaatgaaac catatgttta tattgcaaat gaactaaatt taccatgtgt gatttcttgc
      301 aatcaacttg aatctgtaac taacaaggct attatgaaaa aatattttaa aaataatgca
      361 atacctacaa ctgattattg tattattgaa agtctaaaag atttagaaaa aatacaaaga
      421 ttaaattttc cgcttgtaat aaaacctgtt gatggttatg gttcaagagg tgtatatttt
      481 gtatcaaata tagaagaatt aatagaaaaa tttgaatatt ctaaggaagc ctcacaagat
      541 ggacaagtaa taattgagga attttatgaa agtgaggaaa tacaagggct agcttgggtt
      601 cataatggag agtcacatgt attttatatt ggagatagag agcttgtaaa catacataca
      661 ggaagagctg gaaaaccaga tagattactt tatccatcaa agtattgttt tcaatatgag
      721 gaggaaataa aatctatata tcaaaatata acacaggctt ttgatataaa aaatggacct
      781 ctttatgtac aaatgttagt gggagcatat ggaataaagg tatctgaagt aatgccaaga
      841 ttacctggag gttgtgacta tatggctatt tctcaaataa cagatttaga cataggaagt
      901 ttatttgcaa actttagtgt gaataatgaa gtaaattttg atgaaataaa gaaatataat
      961 atgcatctat caaaatgtgt atatgcatta ccaatataca taagacctgg gacaataaaa
     1021 aagataaata atatagataa aatagaaaac ttagattatg tcactcaatt tctattacta
     1081 gtaaaagaag gagatacaat agaattatat gaagatgaga gacaggattg tggaagagtt
     1141 tatggaatag caaataatat atttgatgca aatataaaaa agaaatgtat tcatgaaatg
     1201 atagaaatac ttgatgaaaa taatgaaaat atgatagaaa atttttaaat aagggagtat
     1261 gatagatatg aagctaataa ctcatgaaca aattatgtca ttaaacattt ctcagaaaca
     1321 aggatatgag tggataaaag aatccttctt aacaaaaaaa gattgtcaat taccaccaaa
     1381 aataagcatg gtagaagaag gacatgtatt ttacaatgta atgccatccc taaatcataa
     1441 tcatatgatt ggtggaataa aaatggttac aagattgcca gggagaaatc caagtttaaa
     1501 ctcagagata tttctataca atatggaaga tgctaaatta aaggctattt tggatggtaa
     1561 ttatattaca gcttttagga ctgctgctgt tgctgccttg gctatcgata catttgctaa
     1621 aaaagatttt aatactattg ctattatggg tttaggtgta actgcttctg ctataatgaa
     1681 tatgctttca tatactctta aagataaaaa tataaatata aagttactta aatataaaga
     1741 ccaagcagag tcgtttgtaa aaaagtatga aaaaaatgaa caatttagtt ttgaaatatg
     1801 tgatacacat gaagaactta ttgtagattc agatgtagtt atttctactg tgacatatac
     1861 agatgaatat ttatcagaat ttaaatggtt taaaaaaggt gctttattat tacctgtaca
     1921 tgttatggga tttggaaatg ttgattatct atttgataaa gtatatttag atgattataa
     1981 tcatttgaaa catctagaaa atatagatag atataaatat tcttgtgaat tttatgatgt
     2041 tttaagtaaa caagctaaag gaagagaaaa tgatgatgag agaataatat cttacaatat
     2101 aggtttgtca atacatgata tattatttgc aaataaaatt tacgaattgt gtgaaacact
     2161 aagctgtgaa gaaatagaat taaaagaacc aaaagaaaaa tattggatat agtttatttc
     2221 attcatattt acaatagttt attgtaaata tgaatatgaa gtaatatatt gttagaaaat
     2281 tagcatgaat aggatagata aatttatgga taaatataat ttaattaggt tcgtatctat
     2341 tggaaacgaa tatataggaa atttagttgc attagaatca aataaagtaa taccttttga
     2401 tataaagaga gtttattaca tatatgatgt accaaacaat gtaaagagag gatttcatgc
     2461 acacaaaaac ttggaacaag tactagtttg cctaaatgga agtgtgaaga taaaatgtta
     2521 tgatggtgta aaagagacta tatttaattt acataaaaat gatgttggat tatatatagg
     2581 aacgggagtg tggcatgaga tgtttgattt tgatgaaaaa actgttttaa tttctatagc
     2641 atctgaacat tataatgaag aagattatat tagagactat aaagaatttt taaagtattt
     2701 aaataatgtg tagttattat acaattagtg atgtattttc ttaattaaat aaatttaaga
     2761 tagccacata gagaatagat agattaggat gtgaatttat tgttggttaa atttaataat
     2821 ttattaaaac agttccaaac tcataaagaa gaatatgaac agaagattaa ggatgtaatg
     2881 gaaagtggat attatatcct tggaaatgaa gtaaagcatt ttgaagaaga atttgctaac
     2941 tatgtaggta gcaaatattg tgttggttta gccagtggtt tagatgcact gataattgct
     3001 tttagagcat taggaataag caaaggtgat gaagtaatcg tgcaatcaaa tacatatatt
     3061 gctagtgtta tgggaatcag tataaatgga gcaacaccag tatttgtaga accaaatgaa
     3121 tattataata tagattcatc taagatagaa gaaaagataa atagcaaaac aaaagctata
     3181 ttagtagtgc atttatatgg tcaagcaagt aatatgaaag atatacaaga attatgtgat
     3241 aagtataact tgtatttaat tgaagattgt gctcaatctc atggagctaa atatgatgga
     3301 aaaatgacag gaacatttgg tgatataggt tgttttagtt tttatccaag taaaaatttg
     3361 ggtgcatttg gagatgcagg agctatagta actagtaatg aggaaatagc tgataaagtg
     3421 aaaattctta gaaactatgg ctcagaaaag cgatattata ataaatttgt cggatataat
     3481 tcaagattgg acgaaattca ggcaggttta ttaagagtaa aacttaaata tctaaatgaa
     3541 attgaaattg aaaaatcaaa aatcgtagat agatatctta atgaaattaa taatcccaaa
     3601 gtcatacttc caaagataga aaataattgt actcatgtat ggcatttatt tgttttacaa
     3661 gttgaaaaac gagatacatt tatggaatac ttaaagaaaa gtggtataga gtctataata
     3721 cattatccga taccaccaca tcta
//
LOCUS       DAGSHT010000271         3699 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.277, whole genome shotgun sequence.
ACCESSION   DAGSHT010000271 DAGSHT010000000
VERSION     DAGSHT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.277"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            23..895
                     /locus_tag="MJB72_003448"
     CDS             23..895
                     /locus_tag="MJB72_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264508.1"
                     /translation="MEMKIAVSNLSKRLGKKQVLDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            892..2181
                     /locus_tag="MJB72_003449"
     CDS             892..2181
                     /locus_tag="MJB72_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264509.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGNIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2111..2755
                     /locus_tag="MJB72_003450"
     CDS             2111..2755
                     /locus_tag="MJB72_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264510.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3022..3486)
                     /locus_tag="MJB72_003451"
     CDS             complement(3022..3486)
                     /locus_tag="MJB72_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264511.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRALKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1387 a          442 c          574 g         1296 t
ORIGIN      
        1 gtttctgggg gtgtatttat atatggaaat gaaaatagca gtatccaatc ttagcaaaag
       61 acttgggaaa aaacaagttc ttgataatat aaatcttgaa atcgacagtg gtatgtttgg
      121 tcttttagga agaaatggag caggaaaaac cacatttatg agaattttag ccactttatt
      181 atcaaaaaca gaaggttcaa tacatatgtg tggagttcct attgagaata ctaaggaaat
      241 tagaaatatg gttggttatt tgccacaaga cttttcaatg tatcctaaca tgacagtata
      301 taaagcaatg gattatttag cagttttatc ggatatacca ctaagaaaaa gaagaaaaat
      361 tatagcaaag ttattaagca aagtaaacct tactaattat caaaatgtga aagtaaaatc
      421 tttatctgga ggtatgaata gaaggcttgg gatagcacaa gcattaattc ataatcccag
      481 agttttaata gtagatgagc cgacagcagg tcttgaccca gaagaacgta taagatttag
      541 aaatttacta tgcgaaattg caaatgatag aatcgttatt ttatctacac atattgttgg
      601 agatatagaa gctacatgtg aaaacgtagc tatattgaat agtggaaaag tcatatacaa
      661 tggctcaaca gaagcattag caaaactagc tgaagggaaa gtatatagta ttgaagtaga
      721 taaaaaagac attgaaaata taaaaagtag atttatagtt ataggaatgt tgactcatgg
      781 tggaaaggct atacttagga ttatatcaga tgataaacct tttgagacag cagtaaattg
      841 taatccaact attgaagatg gctatatgct tataatggga ggagataata tatgaatcta
      901 tttataaaag aatgtaaaaa tatattaaag agtatggtat attatatttt tattgtagtt
      961 ttagtttttt tctatatttc tcaacttgga agttcagttt caaatgatgt aagtaaaaat
     1021 attcctaatg atggaaatcc attagttgct ccacaaaaag tagagaatat tgaggaatat
     1081 tactctgtac acaatcaata tccatatggt caaaaagaat ctatgaaaac agaaaaagtc
     1141 attccaaatg caactataaa cctaattaga gaatatcaaa aaaacacata tacaacgtat
     1201 ccaacagggg tagttaagta tataaaatta gaccaaaata aaacgaagac catagaaaaa
     1261 tatatagaag aaataactgg taaaagtatt agcgaaataa attcactttg gaaaactgat
     1321 gaaagtataa aaatagatta ttctaaagta tctcttgaaa gatttgaaga aataatgcaa
     1381 agagtagacg acattttagg aggagggtct gcatatgctt ttgacaagtt acaaagattt
     1441 ggagtagttc cagttaccta tgatgaagct atgaagaatt acaactatac aatacaaaaa
     1501 gataaagtta gtaatgggta tgcaagaatt ttttgcgatt atataggtat agttttaagt
     1561 atatttccag tatttgtggc agtttttatg tcacttaaag ataagcgttc aagaatgaga
     1621 gagattattt attcaaaaca agtatcttca tgcaaaatag ttttatcaag atattttgca
     1681 cttgtcttta tgatgatatt gcctgtatta attttaggta tgaaagattt acttcctttg
     1741 attttctttg ctaaagataa aggtatatct attgataact tagcatttat aaaatataca
     1801 ttatggtgga tactaccaac tcttatgata gttacagctg taggaatgag tattactata
     1861 ttaacagata caccagtagc aattattgtg caattaattt ggtggcttta cagtataaat
     1921 tctacaggac tcaaaggtga ttattcaaca atggggctaa tgataagaca taatagtgta
     1981 ttaaatggga atattattat acaaaacttc aatcaaatta tgattaatag aattgtgata
     2041 agcttgggag cattggtgct tgttttgtta gctatattct tatatgagca aaaaagaagg
     2101 gggaatcttg atgtttgggc aaagttttca aaactgttta gttctaataa aagaaaactt
     2161 caaattaagc actttaacta atttaatatg ttcaattata ttgctatgta tgattcctct
     2221 aatacaagga gtagctaatt tagacagaat ttcaacagct atatgtttag aaaattatgt
     2281 tgcaataata gggatagtaa tgttggtacc tgtattttcg ctagagcaat caaaagaaat
     2341 tgatgagata gtatcttcaa aagcattatc tcaagttatt gtacaattaa ttaggatact
     2401 gatgtcaatt ataggtatag tggtattaat cataggtttt gtaaatgttt taatatatct
     2461 tggatgtaat tttccagtag ttgagtatat tattggaagt ttcgcatcag ctatgtttat
     2521 aggaagttta ggaatgtttt tctcaaattt atttaataat acaatcatag gttatatgat
     2581 atctatagga tatttaattt taaatatgat gactaaaggc aagtatgttg gaaaattttt
     2641 tataatgtct atgagtcaag gtagttttga tgagaaatat tggctgatac ttggaagtgt
     2701 tgtgttgata attgcatctc tcttaataaa accagtaaaa aggaagatta gataataaaa
     2761 tgagagctta gataaaataa aggtaataca aataaaaagt acatttatga aatataaatc
     2821 tatttttata tacacaaacc tacttacaat ctctttatat atgtagatag atttatatat
     2881 ccgtagtata aaatatatat gtataccgaa ttttatcaaa agctctcaaa tttatatttc
     2941 aaagacactt attgctatga cctacacata tgtatttttt tattttaatt gttgtattaa
     3001 caataagact attgttataa actattgata cctcataaca aaaaattctc catctcctaa
     3061 atgcgttttc tgtaccttag tctcaactat tttaaatcct tgagactcat aacattttaa
     3121 agctctcatg ttccaggttc ttacatttaa tacaattggt ttagtactat atttatttct
     3181 acattcttgt aatcctaggc taattatttc tttaccaatt cccattccac aataattcgg
     3241 tttcatacca ataccaaaga atacagagct tccttcatct aataagttta taaaacctat
     3301 caattcttta ttgtcattta taaaacttat aaaatttttt cttttatctt cttttgctag
     3361 tgaaaaattc ttctttttaa tttcctccca tggaggtaaa ttgtatattg aatatttacc
     3421 ttcatatttc caattagtaa cttcaatagc atgttcttca gtaaatttaa ctttatttaa
     3481 attcatatgt cctcctatta aaatatttaa ggtaattaat ggtattttaa agttttagaa
     3541 aataagtaaa actatttata tttatccata aaatctttaa aaattttact aacaattctt
     3601 gtaccctgta ctttttattg gattcacaga agactataaa ctaattttga attaccttct
     3661 aattcaaaaa catcacgata tatataattt cttagcttg
//
LOCUS       DAGSHT010000272         3667 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.278, whole genome shotgun sequence.
ACCESSION   DAGSHT010000272 DAGSHT010000000
VERSION     DAGSHT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.278"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..890
                     /locus_tag="MJB72_003452"
     CDS             <1..890
                     /locus_tag="MJB72_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBY8264512.1"
                     /translation="NYYLRNTFGEKVFKVSINAGFTCPNIDGSLGYGGCTYCSKEGSG
                     DFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYAPLEVLKEKYETILELE
                     DVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHDSTSKIINRGHDYKTFV
                     EGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVDGIKIHLLHVIKDTPME
                     KMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKRDELVGPIWSLKKWEIL
                     NKIDDTLKARNSYQGCKFV"
     gene            complement(1161..2105)
                     /locus_tag="MJB72_003453"
     CDS             complement(1161..2105)
                     /locus_tag="MJB72_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY8264513.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            2423..>3667
                     /locus_tag="MJB72_003454"
     CDS             2423..>3667
                     /locus_tag="MJB72_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8264514.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKV"
BASE COUNT         1438 a          522 c          502 g         1205 t
ORIGIN      
        1 ggaattacta tcttagaaat acttttggag aaaaagtttt taaggtatct ataaatgcag
       61 gatttacttg cccaaacatt gatggaagtc ttggttatgg cggatgtacc tattgtagca
      121 aagaaggctc aggtgatttt gctggaaacc ctaaagataa tcttatcagt caattttata
      181 atataaaaga aatgatgtta aaaaaatggc ctcatgcaaa gtatattggc tactttcaag
      241 cctatacaaa tacgtatgct cctcttgaag ttttaaaaga aaaatatgag actatattag
      301 aacttgaaga tgtcataggt ctttcaatat ccacaagacc tgactgtttg ccagatgatg
      361 ttgtagaata cttgtctgaa ctaaataaaa gaaccaattt atgggttgaa cttggacttc
      421 aaacaattca tgattctaca tctaaaatta taaatagagg tcatgattat aaaacatttg
      481 ttgaaggtgt tgaaaaatta aagtctaaaa atataaaggt tgtcgtacac attataaatg
      541 gtcttcctgg tgaagattat aatatgatga tggaaacagc aaaggcagtt ggtaaaatgg
      601 gtgtagacgg tataaaaatc catctattgc atgttataaa agatactcct atggaaaaga
      661 tgttacaaaa tggtatgtta actcttatgg aacaagatga atatatcaat cttgtctgtg
      721 accaattaga aatacttcct gaaactatga taatccatag actcactggt gatggtaaga
      781 gggatgaact agtaggtcct atatggagcc taaaaaaatg ggaaatttta aataaaattg
      841 atgatacttt aaaggctaga aattcttatc agggctgtaa gtttgtttaa tacaaaataa
      901 taactataga aatggttata caaatatagt cctttctata gttatttagt atttacaaaa
      961 caaaatatta atttgtacat ttatacaata tataataaaa aatagatagt ctaaactgta
     1021 tctacttata ttttatttgt actgtccact ttattctata aaatttataa atagttattt
     1081 tttaatacca aatataattt ggtataattt ttgttggaat ctacaatgta taccaataaa
     1141 tcctttttaa gtatataatt ctaaatactt tcagtttttt caatctgctt taacttgttg
     1201 gacttataaa atattcttat ttcatcataa gaaagattaa gtgctactac tgcagatatt
     1261 aacatcaaca tcactccaaa taacttccct aaggaaaaac tttctcctaa tatagtgcaa
     1321 ccaattatta ctgaaccaac taattctact gatgccacaa cacctgcaac tgacaactct
     1381 acacctttag atatacctgc tgcataaaaa atatatgcaa gtgcagctgg aacaatgcct
     1441 aacattagca tacaagataa aacatctaaa tccttagcat aatttaagat ttcccaaggt
     1501 cttgaagaag gaatcataaa tatagctcca aataagaaac tgtatactaa tattgttgaa
     1561 cttgaaaatt cctttaaagc atttttacta atagttggca taagtgcata tgctattgct
     1621 gataatactc caagtaaaag cccaaaagcg tttaaacctt gaaaatccaa acgtccacca
     1681 gtcactgcca taattgcacc aataaaacaa agaataagtg aaaataattt gcttctagta
     1741 atattttctt tataacataa ttttgaaaat attgctaaaa acagtggtga tgtatatagt
     1801 aaaactgctg caattgatac acccgcaatg tcaattgctt taaaataaca taaattaaac
     1861 attgcctgac atataattcc tattattact gaatatataa ttcctttttt gtttatcttt
     1921 aagatttgtg gtgtccttat agatgaatat acgcctaaaa ctataaatcc aaaaaacaat
     1981 cttgtaaatg ctacttgttc tgatgtcagt ccatttccca ttaaaaactt tccaaataat
     2041 ccaagtgtag cccataataa ccctgctatt gctataaata catacccttt acttttttct
     2101 cccattttaa attcccttct atttttattt agtcaattta cactcttctt tttaagcaaa
     2161 aactatgcca tcttaaatca atatatatcg actctaaaac cctagtcttt ccaataatac
     2221 taataaattt tcaatttttt tatatttatt tttcgcttac tttaagttga tttagactta
     2281 ttttaattgt aaaatgagtt atatataact tatttaaagt taaaatagac tgaaacttat
     2341 atataaatat caaattttaa gtatattaat caattaaacc tattttaata tatcattaaa
     2401 aatttaggag gaaaatataa aaatgaattt agatttggaa ttttatcaaa aaatattaga
     2461 agcttcccat gatgaaatat gtgtttctga tgataaagga ataataatat attgtaataa
     2521 agcatttgaa gaaaattatg gtctaaaaaa agaagatatt ttgggaaaaa atgtttcttt
     2581 tttagaagac tctggatatt caacaaaaag ccccatacca attgtattaa aaacaaagac
     2641 taaattttca ctggaacaag atactcaaac agggaaaaag cttatcataa ctgctacccc
     2701 tatatttgat gaaaatggac acttagaatt tactgttgaa aattgtagag atataactga
     2761 acttaataat ataaaaaata aacttgaaga cacaaaaaag caagtaaaaa aatataaatc
     2821 tgaagtagaa acactataca gaactgcact tagaattgaa gatactgtaa ttatggacgg
     2881 aatagttatg agaccaatca taaacacagt aaatcatgtt tctaaaacgg atgtaagtgt
     2941 acttttattg ggtgaatctg gaacaggaaa aagctcactt gctagatata tacaccacaa
     3001 cagcaataga tctaatggcc catttataac aataaattgt gcaactatat ctccacaact
     3061 tcttgaatct gaactttttg gatatacttc aggtgctttt acaggtgcaa gtactaaagg
     3121 taaagttggg ctcgtagaat tagctaatgg aggaactctt tttttagatg aaataggaga
     3181 tataccacaa aatctacagg caaaattttt gcaattaata caggatagaa cttttactcc
     3241 agttgggagc ttaaaaaata aaaaggtcga tataagaatt atatctgcta caaatgttga
     3301 tttagtttca aaagtaaaag aaaaaaagtt tagagaagat ttatactata gattaaatgt
     3361 tatagaaata aaattgcctc ctcttagaga aagaagggat aatctagtgg aaattataaa
     3421 atattatttt aataggtact caagtgactt caacctaaat aaaactattt ctaaggaagc
     3481 tatggatgct attgcaaatt atagattccc aggcaatata agagaattac aaaatataat
     3541 tcaaaaaatc ttgcttactt gtacagataa tcatataaca atagatgact taccaaacat
     3601 tcttacaaaa aatatacata ttaccaataa tggaaataaa acacatatat cccaaattaa
     3661 taaggtt
//
LOCUS       DAGSHT010000273         3645 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.279, whole genome shotgun sequence.
ACCESSION   DAGSHT010000273 DAGSHT010000000
VERSION     DAGSHT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.279"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            3..518
                     /locus_tag="MJB72_003455"
     CDS             3..518
                     /locus_tag="MJB72_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBY8264515.1"
                     /translation="MNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMTAQ
                     DLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISNQE
                     LSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYRMSRALK
                     KLELYLEGGKL"
     gene            515..1276
                     /locus_tag="MJB72_003456"
     CDS             515..1276
                     /locus_tag="MJB72_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264516.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1314..2180
                     /locus_tag="MJB72_003457"
     CDS             1314..2180
                     /locus_tag="MJB72_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264517.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2201..3442
                     /locus_tag="MJB72_003458"
     CDS             2201..3442
                     /locus_tag="MJB72_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264518.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
BASE COUNT         1463 a          388 c          539 g         1255 t
ORIGIN      
        1 tagtgaatga tgctcggatt atatcacaaa taaaagcagg aaaaaaagaa ttgtttgata
       61 ctctaataaa aaaatactat gatagcatct actgttattg ttatcgccat gtagacaata
      121 aaatgactgc acaagattta actcaagatg tatttgtaac agttataaat aatatagaaa
      181 attataaaca ttatggtaag tttgaaaact atttgtatgt tattgcaaaa aacaagtgta
      241 aggatttttg gaagaaaaaa caaccaatat atttagagtc attagataag gagatatcaa
      301 atcaagaatt atctgatata gaaaataaaa tgtttataaa agaagctttg actaaattac
      361 cagaattaga atttgaagtg ataattttac ggtattacca tgatttaaga tttaaggata
      421 ttgctaaaat tatggagtca agcgtctctg tcacaaagta caggatgagt agggcattaa
      481 aaaaactaga attatatctg gaaggaggta aattatgaat aataaagaaa taaaaagaag
      541 tctacagaag tataaggttc ccaaatatga ttcttcagct cttgaaaaga ctattaaaac
      601 tatacatgag ttaacgctta gtaatactta taaaagaatg aataataaag acttttttat
      661 atctcaaatt ggttttataa gaaaaagaac ttggattatg caatttctaa tactatgtac
      721 aatatgtata tttttattta gtacatcagc agatagtaat tcattttata atagtttatc
      781 cttgatttct atagcatcac cactttttgt actgacaaat atagaagaat taacaaaagt
      841 gtataatagt agtattttag agatagaatt ttcaacaaaa aatacattac ataaaattat
      901 tgctactaga atgcttatat ttggaataag tgatttattg tttatgatta ttattttggt
      961 atctgcaaaa caaatcattg atgtaaacat tctgtatata ataatttata tactagttcc
     1021 ttttaaccta atgtgtatag gatgtatgga aattataaat agaaataaag gtaagcatac
     1081 aaattattat tgtatgatat acggtatatc tcttgtatta ttaatagtta cactgagtaa
     1141 ctttagagat aatctatatc aatttgaagc aataaatcaa tggctaattg tttttctagt
     1201 cacagcagta acattaatat atgaaataag aaaaacgtgg agacatcttt gttcatttga
     1261 aaatattatt atctaagtaa aaaataagaa tacacagtat caggaggaat agtatggagt
     1321 taagtgtaga acgtttaaca aaacagtata acaataaaat tatagtagat aaaattgact
     1381 ttaaattaaa tgagggtata tatggatttt taggagaaaa tggagctgga aaaacaactc
     1441 ttatgagaat gatttgcgga atattaagtc ctacatctgg agaagtaaag gtggatggta
     1501 aaaataattt ggatatggga gaagatttta gagatatgat tggatacctt cctcaaaact
     1561 ttggatacta cccagatttt actgccaaag aatttatgct atatatagca tcattaaaag
     1621 gattaatgcc tagctatgca aagagaaaaa cacaagaatt attagaaatg gtaggattat
     1681 cagaggtaat taataaaaaa ataagaacat tttctggagg tatgaagcaa aggctaggat
     1741 ttgctcaaac actacttaat gaccccaaaa ttttaatatt agatgagcca acagcagggc
     1801 tagaccctaa agaacgagtt agatttcgca acctaatttc agaattttct aaggaaaaga
     1861 ttgttatatt gtctactcat attgtgtctg atgtagaata tattgcagat aaaattttaa
     1921 taatgaaaaa aggaagattt gttttaactg gctcaataga agaagtaact tcaaagataa
     1981 aagataaggt atggaaatgt gtggtcaatg agaatgaaat agattatatg tctgatagat
     2041 attgtttagc aaatatccat cattctggtg gagttgcaaa tcttagaatt gtaagtgata
     2101 taaagccaca tgacaatgca tgcaatttaa aaccaacatt agaagattta tatttatatt
     2161 gtttcaacga agtactataa atatacataa ggagataggt atgtctacat taattaaatt
     2221 tgagttaaaa aaaataataa aaagaaagtc tacaatagca gttattttag gaagtttatt
     2281 cttaagcatc attctttttg cattaatgcc aattcaggaa aaagctaatg atgaaagtgg
     2341 aaaagagtat aggggtttac aggctattga gctaaaaaag caacaatcta aattaaatga
     2401 gggatacata acagataaaa aagtaaaaaa ctcaatcaga gattaccaaa aaatgtttaa
     2461 taatccaaat aatattacaa cagatgaagg tgggttttca aattttaaac cagatattta
     2521 ttggaaattc ttctatccaa ataaagcttt ttataatttt ataatagagt catatagtaa
     2581 tccaaatgat tataatccag aaataattaa atcaatgcct ttagaagatg gagttaattt
     2641 ttataaattt agaaatgaaa aagtaaatac aatattaaat atggaatatc ctgataaaaa
     2701 ttacacacag aaagagaaag atttttggtt gtctaaaaat aaggatatag aagagcctta
     2761 tttaaatgga tattatcaag gatggaatta ttttctattg gctagagatt tactaatttt
     2821 tactatagta gctgtctgca taagtacagc ttctgtattt tcaggagaat accaatccgg
     2881 tgctgattca ataatattat cttctaaata tggaaaaaca aaagttataa cagctaaaat
     2941 agtaagttcc tttttatttg gtacgattat ttttacaata aatacaattg tgtctattgg
     3001 tatacttctt ttatcttttg gaattgatgg atggaattta ccattacaac ttacaaccag
     3061 ttcaattcca tacccatata catttttaca ggcaatatta ctaagtgtgt taattattta
     3121 tatgatatta atactattaa ttagttttac acttttatta tcatcttata tgaaatcacc
     3181 ttttccagtt ttaattacaa atataatatt actatttgta ccaatatttt taaaatatag
     3241 tgaaacaaat aagttgtata atcaaattct caatgtttta ccatcaaatg ctatttcatt
     3301 ttcatttact aattatgtag attataaatt tggtaattta ataatagctt tacctacaat
     3361 gattattttt acatgcttag ttcttacagt tgttttttta ccttttatta aaaataattt
     3421 taaaaaacat caagttaact aaaatatata ttataaaaag aaggtgtctt aaaatgaact
     3481 ttttagctta ttaggcactc ttctttgtat taatttaaat atattttagt attagattaa
     3541 atatgttttc atcattttaa caatgaaaaa gagctttatc tctattttga gatagctctt
     3601 ataaagttaa atattaaatt gataactata aaatatgtag tttga
//
LOCUS       DAGSHT010000274         3613 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.280, whole genome shotgun sequence.
ACCESSION   DAGSHT010000274 DAGSHT010000000
VERSION     DAGSHT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.280"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..394
                     /locus_tag="MJB72_003459"
     CDS             <1..394
                     /locus_tag="MJB72_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBY8264519.1"
                     /translation="LKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTKRDLI
                     PLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYMINPH
                     IAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            411..1007
                     /locus_tag="MJB72_003460"
     CDS             411..1007
                     /locus_tag="MJB72_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264520.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            1044..>3613
                     /gene="clpB"
                     /locus_tag="MJB72_003461"
     CDS             1044..>3613
                     /gene="clpB"
                     /locus_tag="MJB72_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBY8264521.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     "
BASE COUNT         1519 a          359 c          668 g         1067 t
ORIGIN      
        1 attaaaaaag gatttaaatc aggaatatac agagagcaaa agtttagtta tgaataaaag
       61 taagtatatg aatgtttgtt ttaagaatta tattaaagta gtagaaggaa tgtatactaa
      121 gagggattta attccattaa aattaataaa ctctatggat aaaaataata agattagaaa
      181 aacattatat gaattatctt gtgaatttga atatccaaaa acttctttat cttcattgtt
      241 tacggagctg aaaaaaatgg attttttaca aagagtgaaa aatggtgagt atatgataaa
      301 tccacatata gcatataaag gtagtagagc tgataaagaa agtttattag cagaatataa
      361 tcagataaaa agacctaata aggttagtaa ataaggaaga aggtttttat atgagtttaa
      421 aacttgatag aaatgttcta cagtggtttg actatgtatt tgagaatgaa aaaactagtt
      481 tgagacacta taattttaat tgtacactaa aggagattag ttctaccagt ttgaacaaag
      541 tagcttttat tttagaaaaa aataactcta agtactggaa gctttatttt gaaataccag
      601 ctgaagttac tttaaaactt aagcaaaaca ttcatccttt atttagggaa tatatatacg
      661 aacaaatatc actgtataac aataatcaaa tttacaattt tgtaaattca aacatattaa
      721 aagtgtttaa taatattgct atttatcaat ataatatttt agaaaatcta tacactattg
      781 attttaaaaa gagttttata gataaatgtc agtatttatt aataggtgaa aaaagactta
      841 tagatgaaga cttgtattta atagcaaaat caaaagaagt attcgatttt tttaattcag
      901 atggaacatt taatttaaca ttgagtttcg atatacaaaa aaacgagaat ttacttgata
      961 gtcttttaga gcttagaaaa tcaataatta taaatgagag aatttaattt atttggatat
     1021 aatattaaaa tggagggatt tatatggacg tagaaaaaat gactttaaga gttcaaaaaa
     1081 gtttaaatga ggcttataac gaagcagtaa aaaatcataa ccaacaagta gatgtaattc
     1141 atcttttctc agcacttatt aatcaagagg atggattgat accaaatata atcgaaaaaa
     1201 tgaatatatc aattgattct ttgagaaata ctgtaaactt tgaaattgat aagcttccaa
     1261 aagtatatgg agaaggtgct gactcacaag gagtatcagc tactagaaaa ataaatgagg
     1321 tgcttataaa ggcagagagt atttctaaag aatttaaaga ttcatatata agtgtagagc
     1381 atgttatgct tgcaatgatg gaaacagaat caaaatcagc tgttggtaaa atattaaaac
     1441 aatataatat aaataagaat gactttttaa atattttatc acaagtgaga ggtagtcaaa
     1501 gagtagaaac acaagaccca gaaggcacat atgatgcact tgctagatat ggaacaaact
     1561 tagtagattt agccaagaaa aataaattag accctgtaat tgggagagat gaagaaataa
     1621 gaaggattat aagaatttta tcaagaagaa caaaaaataa tcctgtattg attggtgagc
     1681 caggtgtagg taaaactgca attgtagaag ggttagcaga gagaatagta agaggagacg
     1741 tcccagaagg tttaaaagat aagattgtat tctcacttga tatgggagct ttaattgctg
     1801 gtgcaaaata tagaggtgaa tttgaagaaa gattaaaggc agttttaaaa gaagttcaaa
     1861 gttctgatgg aaaaattata ctgtttatag atgagattca tactattgtt ggagctggta
     1921 aaacagaagg ttcaatggat gcaggtaatc ttattaaacc tatgcttgca agaggtgaat
     1981 tgaactgtat tggtgcaact acttttgatg aatatagaaa gtatatagaa aaagataagg
     2041 ctcttgaaag acgttttcag cctgtaattg cagaagaacc tactgttgaa gatacaatat
     2101 ctatacttag aggtttaaaa gaaagatttg agatacatca tggagtaaga atacatgata
     2161 atgctattgt agcagcagca aaactttctc atagatatat acaagataga tttttaccag
     2221 ataaagcaat tgatttaatt gatgaagctg gagctatgat tcgtagtgaa atagattcat
     2281 tgccaacaga attagatgtt gttagaagaa agttatttac attagaaact gaaagagaag
     2341 cattacttaa agaaaatgat gataaaagta aatcaagatt ggatgatatt caaaaagaaa
     2401 tagcagagtt aaaatctaaa aatgatgaaa tgacagcaaa atatgaaaaa gaaaagagtc
     2461 aaatattaga tattaaaaac ttaaaggctc aattagacga agctaaaggt aaagctgaaa
     2521 agtatgaaag agaatatgat ttcaacaaag cagcagaggt taaatatggt gaaataccta
     2581 agttagaaga acaaataaag cagtacgaag aaaatatgaa tgatggttct gaaaattctc
     2641 ttttaaaaga agaagttact gaagaagaaa tatctagcat tgtatcaaag tggacaggta
     2701 taccagtaac taaactagtt gaaggagaaa gagaaaaatt acttaaacta gaagatgaac
     2761 ttcataaaag agttattgga caagatgaag cagttacagc agtttcaaat gcagtaatac
     2821 gtgcaagagc aggattaaaa gatgaaagaa aaccaattgg ttcatttatt ttcttaggcc
     2881 ctacaggtgt tggtaaaact gaacttgcaa aaactcttgc tagaaattta ttcgatagtg
     2941 aagataatat aataagaata gatatgtctg aatacatgga aaaacatgct gtatcaagac
     3001 tagttggacc tcctccaggg tacgtgggat atgaagaggg tggtcaactt acagaagcag
     3061 ttagacgtgc tccttattct gtaatactat ttgatgagat agaaaaagca catgaggatg
     3121 tatttaacat gttcttacag atattagatg atggtagact tactgataat aaaggtaaga
     3181 cagtagactt taaaaatact cttataataa tgacttctaa tataggaagt aattatctat
     3241 tagaagcagg aggaaatata acagagacta ctaacaattt agtaatgaat gaaatgaagc
     3301 atagatttaa acctgagttt ttaaacagag tagatgatat tataatgttt aagcctttag
     3361 accaagaaaa tatcaagaaa attatagata tatttatgaa agatttaaaa aatagattaa
     3421 aagaaaaaga tattactatt gaagttacaa attcagcaaa agatgttatg gtgagagaag
     3481 gatatgaccc tgtatatgga gcaagacctc ttaagagata tataggaaat acattagaaa
     3541 ctataattgc aaagaaactt attgctggag atgtgtataa tggatgtact atagttatag
     3601 atggaaaaga tga
//
LOCUS       DAGSHT010000275         3595 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.281, whole genome shotgun sequence.
ACCESSION   DAGSHT010000275 DAGSHT010000000
VERSION     DAGSHT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.281"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(16..396)
                     /locus_tag="MJB72_003462"
     CDS             complement(16..396)
                     /locus_tag="MJB72_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8264522.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(597..1788)
                     /locus_tag="MJB72_003463"
                     /pseudo
     CDS             complement(597..1788)
                     /locus_tag="MJB72_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(1851..3107)
                     /locus_tag="MJB72_003464"
     CDS             complement(1851..3107)
                     /locus_tag="MJB72_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBY8264523.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1295 a          685 c          376 g         1239 t
ORIGIN      
        1 taaaaataat aacatttata cataagcttc ttcccaattt actacccagt tttctaacga
       61 ctttatcttt tcctctgtta gctcctcttc gtgaaatgat actaataaat tccttataaa
      121 attattcttc aaaagatttt gcacatttct tgatataaac tctaaatatg ccttttcttt
      181 tattgcaact gtataataca tacacttgcc tgtttcttga acatataaga atcttttctt
      241 tgatagtttt gataaagttt ttaaagctac tttttctggt aaattatgtt cttctttcat
      301 ataatcaata atctcatatg aattggtttt tatatctaaa ttccaaataa atttcataat
      361 aactaactct aattttgata atttttttaa acacatagtt atcactccgc ttctttatct
      421 atataattaa atattacaat aataatttca caaaatcaat tctaataatc ataacatacc
      481 ttaaaatctg ttcttaactt ctaccttctt aatctcccaa atgatttttt tatattgtat
      541 tcaatagtat aatccttttc caaaatataa cttaccagtt aaaataatct ttatttttac
      601 ttactctcgc cttttacaga agttaataat tttttataag ttacatcaat tccaaaagct
      661 ccaagaggtg cagttataag tatagctaga actgctaacg tcaaaataag ttgtccagat
      721 gccatcccca ttgcaagtgg tacacctcct attgctgcct gtactgtagc ctttggaata
      781 tagccaatca tacagaacaa tctttccttt attgtcaaat ttgtcttaat aaggcagaag
      841 aatactccta ccatacggaa taaaagaact ccaaatatta aaattacagc tccaattcct
      901 gctttaaatg cataacttat atctactgtt gcccctacca aaacaaacaa gagaatttca
      961 gcaccaaccc atagtttcga atactttata gacaatcttc ttgcaactac acgatatttt
     1021 atctgaagga aaatcccaat actcataact gctaataatc cagaaaatgg cacaataccc
     1081 tctaaagcag tctctaaact tatcattaaa aatgagatac ttaaaagcaa cactccttta
     1141 gcagaatctc tcatatgaat cttcttaaaa aatactgcta aacagattcc aatcactgct
     1201 ccaactatta cacctaatat aattgatact ggaatttgta aaaaactaat agctgatata
     1261 ctatttcctt gtgccaatcc tgtgaatact gtaaacatca caattacgaa aatatcgtct
     1321 atagaagttc ctgctaaaag catctgtgga atactttttt ctgtaccata accttcttcc
     1381 attaatttaa gcattttagg tacaataatt gcgggagaaa ctgctcctac tactgcaccc
     1441 atgactgctg cttccaatat agaaacgcct agtaactttg gtgctaaaac tatcattccg
     1501 acaatttcaa aagtagctgg aataaagcac ataagtacag caggtcttcc cactttcttc
     1561 aaatcgttta tatctaaaga taatccagcc cttgttaata taataatcaa tgcaatcttt
     1621 ctaaggtcag aagaaatatc tagtattgaa ttatcaatca aatttaaaac atatggacct
     1681 aatataattc ctgtaagtaa cattcaataa gttaggtaac tttatcctct taaaaatacc
     1741 tcccgaggcc attcctaata aaaaaatcaa agctaaactt gtcaacatat tcctacccta
     1801 tcctttctct aatattcttt ctcttctaat catacattat gttgttttat ttattgtttt
     1861 gttgaaattt tttgtagtga ataaatagat ttatccatat taaatatata tgaaatagcg
     1921 catactacaa agaaataagg taaatagctg tacccaaaaa cttctgcccc tataaataca
     1981 ggtgcaaaaa aagtatttgt agcactacca aagacactag catagccaag cgctgcaact
     2041 aattcaattg gcaaattaaa aaatcctgct aacaatacac caagactagc tccaatggaa
     2101 aacaaaggtg taacctctcc tccttgaaaa cctgctgaca aagtcagtat tgtaagtata
     2161 aactttaaca gccaatcaaa tgaataaatt tctccaccat agaaactatt ttgaattaaa
     2221 tttgtcccta aacctgaata tcttccttta taaaataaaa ggaataatac actaagacat
     2281 actccaataa tagcaattct tatcatagga ttttttaatc tattacctat ttttcttttt
     2341 gacaatttca gacaccatgc aaatgctcca ccaaccattc caaaaataat tcctaaaaca
     2401 ataagcttcc aaaatattga caagtcaaat acaactttag aagaaagtgc aaaagaaaat
     2461 ttctctaacc ctaaagcttt tgatactgaa cttgctgtaa aagaagcagt aaacgcagga
     2521 aaaagcgatt gatactccag tgaaccagct accaaaacct ccatagcaaa tagtattgct
     2581 gctattggtg tctcaaataa tcctgcaaaa ccagctgcca taccagttac tagaaaaata
     2641 ctacaagcat tttttatagg caacctttta cctacccaat gtgaaaaagt tgcaccaatc
     2701 tgaacagcaa ctccttctcg tcctgcacta ccaccaaata aatgggttaa ccatgtacct
     2761 gatattataa atggaaccaa tcttaaagga ataatttctt cttctccatg ccctacctca
     2821 aatattagat tcattccttt actacttttt cctccaaatt taaaatagca ataagcaata
     2881 acaactccta ctactgctaa aaatggtatc agatacattg gatatgtttc tcgaatatct
     2941 gtaactttta gaagaaccgt tccaaatatg gtgtctataa gacctataat tgcaccaact
     3001 ggtataccta tgagtcctaa aaaaaatagt ccaccatatg ttttataaac gcttttaatt
     3061 cttattatca ttttaatcca tattaaagtt ttaaattttt ctatcatttt aattcctttc
     3121 tatttgcatc ataagatttt ttgaaagttt tattgcatca actgtagtca atgtaatgac
     3181 tatcataatt gttttttaaa ttgataggat ataggtacac tcttaaccca ttcttaataa
     3241 aataattaac atataatcta ctattttttc tattaattaa atatttcttc aaatttaaaa
     3301 aataaaaatt tagtaagtac ttcacctcca ttttattgat ttaaacaaca aaaaaagctg
     3361 gtggcatcta tgatatcata gaagtcatca gctcattggc agtttaggat attatcatcc
     3421 aagggagaac ctcattccca taattattat ataatttttt atttttcata tgtctatttt
     3481 atgtaaattg tatttttaat gttataaata atataaccta tcaattaaaa tagtaaagcc
     3541 tctaatatta ttagtgttca atatttcata ttatttttat gatatataat ttaaa
//
LOCUS       DAGSHT010000276         3528 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.282, whole genome shotgun sequence.
ACCESSION   DAGSHT010000276 DAGSHT010000000
VERSION     DAGSHT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.282"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            313..960
                     /locus_tag="MJB72_003465"
     CDS             313..960
                     /locus_tag="MJB72_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBY8264524.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1127..1696)
                     /locus_tag="MJB72_003466"
     CDS             complement(1127..1696)
                     /locus_tag="MJB72_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264525.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1701..2600)
                     /locus_tag="MJB72_003467"
     CDS             complement(1701..2600)
                     /locus_tag="MJB72_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY8264526.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2611..2760)
                     /locus_tag="MJB72_003468"
     CDS             complement(2611..2760)
                     /locus_tag="MJB72_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264527.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2753..3337)
                     /locus_tag="MJB72_003469"
     CDS             complement(2753..3337)
                     /locus_tag="MJB72_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBY8264528.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1257 a          581 c          385 g         1305 t
ORIGIN      
        1 aaacttataa aatttaaaca agttattcat aaaataaaat aataatcatc tataacccac
       61 aactttattt tatattaaat aaaaagtatt agtggttatg ttttataagt atttctttat
      121 tcagttattt attaaaaact aatagtaaat cttatactaa tcgcatattt atgttataac
      181 tatattgtaa ctattttcgt aatcttagga aaagattact tataaaatta accttataca
      241 ataatatatc gtataatctt aattagcata atagttacaa tattaaaatt taaatatggg
      301 agtgatttta gtatgataag acaaacacca ttagagaaac gatatgacaa gctaggtcct
      361 catattgtaa cagcactcaa aaaacgttat tttgatgctt attactgtaa aactagaaat
      421 gaagctttgc aacaaatttt agatttaatt cctcaaaatc atgtagtatc atggggagga
      481 tctgaaacac ttaaagaaat gggcgtacaa actgctgtta gagagaaagg atttaaagtt
      541 attgatagag atttagcaaa aactccagaa gaaagaactg aaattatgcg tcaagcactt
      601 ctatgcgata cttttttgat gagtagcaat gcaattagtg aagatggaca actctttaat
      661 attgatggaa atggcaatcg tgtagctgca atgatatttg gtccaaaaaa tgttatagtt
      721 gtagctggta tgaataaaat tgtaaaaaac attgatgatg caatacaaag aactagaaca
      781 attgcatctc cagcagttgt tcagcgtttt caagatgtca atacaccatg ctatattaat
      841 ggaagttgta tagattgcac aagtcctgaa agcatttgtg catacatggt tactacaaga
      901 atcagtcgtc cagcaaaaaa aataaaggta atattggtag gagaaaatct tggtctataa
      961 aatatacata catgtaaata gctctataaa taataaaatc atatgaaata acttatttct
     1021 aaaagtggta gaataaaagc ttctacaaga ctttcattct gccactttta tattctttta
     1081 atttgtgttt ctatattatt aattgctcca attttcatat atattactaa ctatttttat
     1141 tttcataatt tttttgttct tcatcaagct gtttataata atctttatta tagtcagcta
     1201 gataatctat accacctcct gtaatcacat ttaataattt cattgcagat tcttgattaa
     1261 ctttacaaac ataattatca ccagatttaa caagctctaa tgtaatatta aattcttttt
     1321 tctttgcaga atcaatatct ttcatagttg gatttggcat catttctcct agtttaggag
     1381 gattcttttt taaaatagaa accatatcag tattgttaat ttttaaatct acagtcactt
     1441 tatccttatt attttttata tctgaaattt cccaagtcat atttttagaa tacattttaa
     1501 atccttcttt atcttcgtat ataagattac ctaaccctga ttttgggaaa tacttatcaa
     1561 cagtatctgc atcttgtgtt tttattgaat taaacatact atccacttca ttttttatct
     1621 tattatcatt agtacaacca gctaacacca gtactgatat tgcaaacatt ataatacata
     1681 gaataaattt cttcattaaa ttatatcccc ctatactttt ttgcacaaat attattctct
     1741 tttttatctt ttgaaaaagt tgtagtaatt gcattttgtg gacaaacttt tatacattca
     1801 ccacacctaa tacagtctgg actatttaca tctttatata ttggtatatc aagcttacat
     1861 tttgtttgac atgctgtaca atttgtacat ttatctttat caattttaaa tctatatatt
     1921 gaaataggat taaaaaaacc atagattgct cctaaagggc atatatactt gcaaaatggc
     1981 ctataagtaa tgattgacat caataatatc aataacaaaa tacttaactt ccagctaaat
     2041 aaaaatccta ttgattctct taaattctca tttcctaaaa taagtggtat tcctcccaaa
     2101 agtgttcctg aagggcaaat ccatttacaa aaccaaggat ttccaccccc tgctacattt
     2161 acaatagcaa ctggcaaaat aattacaaat attattaaga ctatatactt tagatatttt
     2221 agatatttat caccataaac tttttttatc tttactggaa agggtacttt atgtagtaaa
     2281 tcttgtacaa gtccaaaggg acaaagccaa ccacaaacaa atctgccaaa aaaagcacca
     2341 aatgccatta aaaaaccaac aacataaaat gagaatttat actctctgct tgataaaact
     2401 gcttgtaatg aacctattgg acatgctcca agtgcacctg gacatgaata acaattcaat
     2461 ccaggtacac ataatttttt actttctcct ccatatatag ttccttctaa aaatccattt
     2521 acatatccat ttgttaatgc tgtaaatgca acttgagtaa taagtcttat attattattt
     2581 actttagata ataacttcat tcctcttctc ctaaccaata ccaatacact caagacaaat
     2641 cataattgct ttagttaata ctgtatttgt ttctccacga tatgtaccta atgcaataaa
     2701 tataatacta attgcaataa tagaaatgcc ttttttatta tctttaataa agttactcat
     2761 tggcttttac catttttagt ctttcatcta taattttttt ccaatcttcc ttactatttg
     2821 cacctacaac cttctcaccc acaaagtttc ctttcttgtc tacaaaataa gttgttggaa
     2881 actccataac tcttttaaaa tcagtttttt taagttcttc agatacagtc aattgttggt
     2941 aagttgcatc tgactttttc ataatgtctt taactaaatt tttctcttca tttgaaagac
     3001 cagttctcat atcagtttta tcaacttcta caactaatcc ctttatagct acattggaat
     3061 tattactctc atattctttt gataatgctt caagctctgg catttcaccc acacatggtc
     3121 cacaccaagt agtccagaca ttaataagag taagttcttt atttgaaaat atctcttttg
     3181 ttaccttatt tccatcaaca tcctccgtat ctaaattact aaaccattca ctattattct
     3241 tgtttttttc tactggctta gattcttttt tgctacatcc agaaatcata cctaaaacac
     3301 aaatacatcc taaaactact atgaaaaacc ttttcatatc tcacactcct agaatttata
     3361 tagtatataa aatcgctgct tgaaataaaa ataacttatc ttctatatta aagtaattat
     3421 atttaataaa taagagctaa tttaaaaaaa gatatacagt taaaatctat ttcttttcag
     3481 acaaaatctt atatatatat aatagcttgt aaatcttaag attatata
//
LOCUS       DAGSHT010000277         3524 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.283, whole genome shotgun sequence.
ACCESSION   DAGSHT010000277 DAGSHT010000000
VERSION     DAGSHT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.283"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            746..1498
                     /locus_tag="MJB72_003470"
     CDS             746..1498
                     /locus_tag="MJB72_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264529.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1488..>3524
                     /locus_tag="MJB72_003471"
     CDS             1488..>3524
                     /locus_tag="MJB72_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264530.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIG"
BASE COUNT         1324 a          516 c          459 g         1225 t
ORIGIN      
        1 agttctttat tatactacag acttttatta ttacagcatt tctgttacat tgcaaagtct
       61 tatttattac agttctttat tatactacaa acctttatta ttacaacatt ttattatttt
      121 aacttcttta ctataatata gtaatatttt aatatctcaa aataatatca atcacttttc
      181 agcatagtct aaaatacttc cactttctaa aattatctat catacctata ctaataatac
      241 tttttaacct ttttattata aatttcaatt gtttttaaca acctatttat ttccaaaact
      301 ccaatatatt ttctcaatta cattgtgtta ataaccccta aatcctttca aattatttac
      361 aatacattcc attaaataat cttactgctc tataattctt tttttctttt taaccctact
      421 atacattatt tacatcattt ttttcaatac aacattcgtt attctataac ctttttactt
      481 ttactatata ccttttttct aactacacct tcaatgttcc acatcatatt actttaccac
      541 aacaattaat acctaaaaat aaccctctaa atcctccaat aatataatca atttaactat
      601 aataataacc tctcacacta tccaaacaat atattttcaa acattacaaa aaaacatcca
      661 actattacaa tatagctcat atccctcctc ccctttcaaa tgctatcatt gacttacaag
      721 ataaatgaaa gtgaggatga tatatatgct taaagtaaac caattatgta aatcatataa
      781 aacaggtagt aaaacatatc ctgtattaaa aaatatttct tttgaaatca acaaaggaga
      841 atttgtcgca atcatgggtt catcaggaag tggaaaaact actcttttaa attgtatttc
      901 atgttttatc ccaattgata atggggaaat aattcttgga aataataata taagaaattt
      961 aaaagaaaag gaactttcag aagttagaaa ccaacaacta ggatttgtat tccaagattt
     1021 catgctattt gatggtctta gtgtctttga aaatatatgt gttccacaaa ttatcaaagg
     1081 tagtcaagtc ttacaaatgg aaaaccgtgc aattaatcta tgtgagctat ttggtattga
     1141 acatatcaga aaaaaatacc ctgccgaaat atctggtgga gaaaaacagc gtacagcagt
     1201 agcaagagca ctcatgaaca acccagattt aatattagca gatgaaccaa ctggtaacct
     1261 tgatagtaag tcttgtcgta cagtcattga atcattttta aaagcaaagc aagaattaga
     1321 tgctactata ctcatggtta cacacgatag ctttgcagca agcttctgtg accgagttat
     1381 tgtgcttaaa gatggaataa tttataaaga agttgtaaaa tcaaattcaa gacataaatt
     1441 cctagatgaa ttactagaag ttttaaaaga aatgggaggc gctcaagatg acaattaata
     1501 aattatacag taaacttcga gcctataatt ggaagaacta tacaacttta ttcttttgta
     1561 taatactttc tgtggtatta gtaacttcat atgctctaat ttattttagc ccaacaatac
     1621 aaaacatact tccaactggt ggagatagca gaaaacaggc tacaatgata ttctcagttg
     1681 ctatattagg atgcgctgtc tttactacat acgcttcttc cctcttttta aaatttaaaa
     1741 gcaaggaaat tggtattttc atggctctag gaacagataa gaagaaatta aaaaaacttc
     1801 tattttcaga gatattgtta atttcaatag tatgctgttt tttaggtctt atattaagta
     1861 ttcctgtatc ttttggtata tggaaaatat ttcaattagt gattattgat accagagaaa
     1921 tgacatatca ttttggaact caaggattca tatatggatt tatatttagt gtatttgtga
     1981 cattatgcat atttatactt tctgtaaaat ttattaatcg tacaaatata attgatatat
     2041 taaatgacca gagaatatct gaaccaatac aggatgtaaa ggactggtat ggtatattag
     2101 gtataatttt tattatactc ggtatttttc taggatatct cgtaccttca ctaactataa
     2161 acaaattaaa ttatagacta ccatctgtat ggagctttac ttatctactc tctcttattg
     2221 gaatatatat gattatgaca tacatagttg tacatgctaa aagaggaaaa catccagaga
     2281 aatactataa aaacatcata tcaactagca tgatgagatt tatgggaaga caaacagtaa
     2341 aaaacatgtg tgttatttca tttttgattg caggagcatt atttgctgta ttctatgttc
     2401 ctaatatggt tacaggtatc tatgatactc ttaaaaataa ccctatagat tatactttta
     2461 cctacaatca aagagataaa caggtatcaa aaaaagatat tgaacaatta gcagataaac
     2521 acaatataaa aattcaaaac tatatggaaa ttccttctat agaattaatt gttgatggtg
     2581 tagaatcaat atatcataaa gatggaacta tcgaaaaggt atatatgaaa aaaagctcct
     2641 atgatatgtt ctttaattct aaagattttt ccaagctaat tggcaaacca ataactataa
     2701 aaaatggtga atatcttgct gttgtaaata aaggaaatga aaatcaagat tataaatata
     2761 ccaaatttat atcaagccca gtcacagaaa aagaaatccc ctttagatat gctggaaaaa
     2821 taatatttaa tggttctttt tttcaaaata gaagtatgaa cgcctatgtc cttaatgatg
     2881 aggattataa atcatttact aaagatttat ccataaaaaa ttacaataat tctatactat
     2941 ttaatgtaga taaagatacc tatgcttttg ccaatgactt aaagaaagaa attataaaaa
     3001 gaacatctaa ggaaatgggc atgttcagtg gttatgatga atacgtaaaa aaacgttatg
     3061 aatcaactgg aagaaaatat tttatggatg aagaatatcc aagtggtgaa ggtcatttaa
     3121 aactagaccc aaacaacaat cagttgtatc ttaattggaa atactatcct aactttaaaa
     3181 ttttacaaag ccaagatatg ataaaaaaca tggcagtctt cttaatgtta ttcatatatg
     3241 tggcaatcat atgttttaca gcagtaggaa tcattgcata tacaaggggt gttactatag
     3301 ctataaatca caagcaggtc tttatggatt taaatcgatt aggagcaaac aatgattata
     3361 taaagttctg tattagaaat caattaaaga aagtattttc ataccctctt attgcaggaa
     3421 gttttttaat ttatctgttt aattttatga ttatgttaaa taatgatggt agaatcgtca
     3481 atagtgaggt caatgctctt ttgataaact taggttttat aggg
//
LOCUS       DAGSHT010000278         3514 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.284, whole genome shotgun sequence.
ACCESSION   DAGSHT010000278 DAGSHT010000000
VERSION     DAGSHT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.284"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1564
                     /locus_tag="MJB72_003472"
     CDS             <1..1564
                     /locus_tag="MJB72_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBY8264531.1"
                     /translation="KLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMYNSYTVV
                     CGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGINIININK
                     NDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTGLKRDIT
                     GIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRGFLNYDT
                     STLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGKVSIVAG
                     SKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERLNKLLNS
                     SDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWKNRFVIT
                     PHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTYINPTGN
                     SCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVNATDLIS
                     NISLTMKELLDI"
     gene            1742..2188
                     /locus_tag="MJB72_003473"
     CDS             1742..2188
                     /locus_tag="MJB72_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBY8264532.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2209..3252
                     /locus_tag="MJB72_003474"
     CDS             2209..3252
                     /locus_tag="MJB72_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBY8264533.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1425 a          329 c          669 g         1091 t
ORIGIN      
        1 taaattaagc agaaatgttg atactgcatg tgtagaagaa gtagggattc caatgattgt
       61 attgatggaa aatgcggtta tatctgcaat gaaaaatatg gatatagaca tgtataatag
      121 ttatacagtt gtatgtggtg ttggaaataa tggaggagat ggtcttggaa tagcaagaca
      181 tctaaataca tatggaaaag aagtaaacgt atttttagta ggtaatcttg aaaaactaag
      241 tgaatgctct aaaataaatt acaatgtact tttaaatatg ggaattaata ttataaatat
      301 taataaaaat gatttaagtg aaaaaaatta tggagatgaa aatgaagtag ataaaattat
      361 taaagttgag aaacaaaata ttataaaaaa taaaaattta acaattttta aagatagagt
      421 atctaaaagt gatgttgtag tagatgctat atttgggact ggattaaaac gtgatataac
      481 tggtattttt aaagaagtta ttgacatagt aaacgataat agtaaaaata catattctat
      541 agatattcct tcaggtatag atagcgataa tggaaatgta ttaggaactt gtattagagc
      601 aaacaaaacg attagctttg agttttataa aagaggtttt ctaaactatg acactagcac
      661 acttataggt gatgtagttg tagaaaagat aggtgttcca gattttatta caagaaaata
      721 ccatgatgaa gagttcataa ctgataagac ttttgtaaaa aataatatta gaagaagaga
      781 taagcatggt tttaaaagtg attttggaaa agtatctata gttgcaggtt caaaaggatt
      841 ttatggtgca agtttcatag caactgaatc tgctgttaag agtgggagtg gacttgtaac
      901 attaattagt agtgaagatg tgcaagaaaa ggtatctatt agacttacag aagcaatgac
      961 tttaaatttt gaagaagaaa gattaaataa attacttaat tctagtgatg caattggttt
     1021 tggtccaggt atgggagata atagtcaaac atttgataaa ttacttaaga tagtagaaaa
     1081 ttctaattgc ccaatagtat tagatgcaga tggattgaac gttatgaagg acagatgtta
     1141 taagttttta gaatggaaaa atagatttgt aattacacct catttgggag aaatggccag
     1201 gttgacagga gattcaattg ggtatataag agaacataga gtagatgttg caaaagaatt
     1261 tgctcaaaaa tataatctgg tggtgctttt aaaaggttat caaactgtca taacagatgg
     1321 taaaaaaaca tatataaatc ctacaggaaa tagctgtatg gcaactggtg gaatgggtga
     1381 ttgtttgctt ggaatgatta cttcatttat aggtcaaggt atggatatat tggaagctac
     1441 agttagtgga gcatatatac atggctatat aggagataaa ttgagcaaag caatgtatac
     1501 ggtaaatgct acggatttaa taagtaacat ttcattaaca atgaaagagt tgttggatat
     1561 ataattggtg atatttgata ctactttgta acaattttaa caaagtatgt tgatataata
     1621 ctattaatta attaattaat ttattttatt gttttgtaat ttaaatttat attcaaaaac
     1681 attgtataaa catataattt atatagggtt ttataattca tatgaaaaga gggtagatag
     1741 tatgaagtta atatggttga tagtagcagt cttatttgga atagcagaaa tgttaacacc
     1801 aagcttaaca ttgatatggt ttagtgtagg agctgtaata cttatatttt taagtagctt
     1861 tatagagagt attttcttac aaattttaat atttgcagta atttctatag caatgttagt
     1921 cgtagctact aaaaaaatag taaaaaaaga taaaggctat aaatctaaca ctaatttaca
     1981 agcaatgatg tctaaaaaag gtattgttac agaagagatt tctccaaata acacaggtct
     2041 agttgtagtt gaacatgaaa catggacggc tatttctata gatggtgaaa agatagaaaa
     2101 aggttctaca gtagaagtat taaaaattga aggggtaaaa ttagttgtta aaaaagtaga
     2161 cgccacagtt aatgtgacaa accaatagaa taaaggagga tttatagtat gggtactaaa
     2221 attgttttaa gtattgtatt aatagtagta gtcgtagcaa taagtttaac ttgtataaga
     2281 gttataaaac aatctaaagt aggtataata atgagacttg gtaagtttca aaaagtagct
     2341 gaaacaggtg tacatttctt aataccattt ttagataaaa tggcatatgt aattgacctt
     2401 agagaaattg taatagattt tccacctcaa ccagttataa ctaaagataa tgtaactatg
     2461 cagattgata cagttgtata ttataaagtt acagacccag ttagatatgt gtttgagata
     2521 gctaatccaa tagctgctat tgaaaattta acagctacaa cactaagaaa tataattggt
     2581 gaacttgatt tagatgaaac attaacatca agagatataa taaatgtaaa aatgagaact
     2641 atccttgatg aagcaacaga taaatggggt ataaaagtaa atagagtaga gttaaaaaat
     2701 ataatgcctc ctcaggatat tcaagttgca atggaaaaac aaatgagagc agaaagagaa
     2761 agaagagaag caatacttca agcagaaggt aataagtcag ctgcaatact tcaagctgaa
     2821 ggtgaaaaac aatctgctat attaacagca gaagccaaaa aagaagctat ggtacgtgta
     2881 gcagaaggtg aaaaagaatc tgctatatta gtggcagaag gtgaagctga agctataaga
     2941 cagacagcta ttgctaaggc acaaggtgaa gctgaaatga taaaaagaac tcaaatggca
     3001 acagcagaag gtttaaaatt agttttttca gcaatgaaag aggctgatat agacaataat
     3061 attctagcat taaaatctat ggaagctctt gaaaaaatgg ctgaaggtaa gtcaacaaaa
     3121 ctggttttac cttcagaagc agttaatttc ctaggaacat tcaaaggaat aaaggaagtt
     3181 atgagtgatg ataacaaaga agtacttgat ataaaagaag ttttaaatga taatgaatca
     3241 ttaaaaaaat aaaagagttt tgttatcata ggaaggatat ggaaattaaa ggtcgaatta
     3301 attaaatata gcacaaaaat gcacgttgaa aagaagagga cttataaatt tttaagtctt
     3361 cttctttgtt tttattagaa agatgaaatt ataaaaataa attaacagaa tctttataca
     3421 atttatcctt tactaatgta aaaagtgtta tataatatta tataaaacat atagcaaata
     3481 agcatggttt tagttagtct atttatatta ttaa
//
LOCUS       DAGSHT010000279         3502 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.285, whole genome shotgun sequence.
ACCESSION   DAGSHT010000279 DAGSHT010000000
VERSION     DAGSHT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.285"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1256)
                     /locus_tag="MJB72_003475"
     CDS             complement(<1..1256)
                     /locus_tag="MJB72_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBY8264534.1"
                     /translation="MRYRYRDDEEMKYSGVEWIGKIPKDWEVKRIKHLFELKKDKSDE
                     ENPTVLSLTQKGLKIRDVSNNEGQLASTYVGYTKIEKNDFILNPMDLISGYTDKAEIE
                     GVISPAYTTFRSKNKVNINHDYYKRYFQMHYHHNFLFPWGEGVSFEHRWTLKNEVFLN
                     LPVITNRLEEQEKIANFLDEKTSQFEFIISKKEELIKKLEEAKKSLISEVVTGKVKVV
                     KTDDGYKLVKRSSEEMKDSGIEWLGEIPKDWEVKNFKYMFTLNKGLSITKADLKESGI
                     PCVNYGEIHSKYRFELKPSKHKLKYVDESYLKSNSISLLKYGDFVFCDTSEDIEGCGN
                     FTYLNENNKVFAGYHTIIARTLEQVNYRYMSYLFDSNDWRIQIRTKVSGVKVFSITQS
                     ILKGTKVILPDLLEQRNIAQYLDSKC"
     gene            complement(1256..3283)
                     /locus_tag="MJB72_003476"
     CDS             complement(1256..3283)
                     /locus_tag="MJB72_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBY8264535.1"
                     /translation="MSIDNNFNHIAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIVKSVYKEYDFLPEEEKDELVIVDLKENHNIDLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNSVKEIIGKFKFKDEIAQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNEMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEMLFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKVNPSAIVNVYGQELLDESFAICQADMIMKGQNPDNIRLGNT
                     LTQDRFSGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIEKDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEDKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEEIKEI
                     YERFEESENSKIFDNEGFGYRKVTIERPLKLSFRVNEEAIENVKNTTQFINLSVSKKK
                     DEEVKVKEEAEGRVKQDKLLKLLESFDSEFEYMKRDKFIKDLKSKSKLYDVALSAGLI
                     KAIVNAIGVRNEDAVVCKDAKGNIESDSSLKDTESIALKEDVYEYFEKEVKPHVEDAY
                     IDESSIDNIGYEIPFTRYFYKYEKLKSFDDIMKEVESLESEIALEIRKVLG"
BASE COUNT          992 a          685 c          321 g         1504 t
ORIGIN      
        1 ttacatttac tatctaaata ctgagctata ttcctttgtt ctaataaatc tggtagtatt
       61 acttttgtac cctttaatat tgattgagtt atactaaaaa ctttaacccc tgataccttt
      121 gtgcgtattt gtattctcca atcatttgaa tcaaatagat acgacatata tctatagttt
      181 acttgttcta aagttctagc tattatagta tggtatccag caaacacctt attattttca
      241 tttaaatatg taaaattacc acacccttct atatcttctg atgtatcaca aaaaacaaaa
      301 tctccgtact ttaataatga aatagaattc gattttaaat atgattcatc tacgtacttt
      361 aatttatgtt tactaggctt aagctcaaac ctatattttg agtgtatctc tccataattt
      421 acacacggta ttccactttc ttttaagtct gcctttgtta ttgataaacc tttatttaaa
      481 gtaaacatat acttaaagtt cttcacttcc caatccttag gtatttcacc taaccattca
      541 attccactat ccttcatttc ttcactactt ctcttcacaa gcttatatcc atcatcagtc
      601 ttaacaacct tcaccttacc agtaacaacc tcacttataa ggctcttttt agcttcttct
      661 aactttttta ttaattcttc tttcttagat ataataaatt caaactgtga agtcttttca
      721 tcaaggaaat tagctatttt ttcttgttct tctaacctat ttgttattac tggaagattt
      781 aaaaatactt cattttttaa tgtccatcta tgttcgaatg ataccccctc tccccacggg
      841 aataagaaat tatgatgata atgcatttga aaatatcttt tataataatc gtgattaata
      901 tttactttat ttttacttct aaaggtagta tatgccggac taattacacc ttcaatttct
      961 gctttatcag tatatcctga tattaaatcc attggattaa gtataaaatc atttttttca
     1021 atcttagtgt atcctacata tgttgatgct aattgtccct cattattact tacatctctt
     1081 atttttaatc ctttttgagt taatgataat accgttggat tttcttcatc agatttatct
     1141 ttctttaatt caaataaatg ttttattctt tttacttccc aatccttagg aatcttacct
     1201 atccactcaa ctccactata tttcatttcc tcatcatctc tatatctata tctcattacc
     1261 ctagcacctt ccttatctcc agtgctatct cactttctaa actttcaact tccttcataa
     1321 tatcatcaaa gctttttaac ttttcatact tataaaaata tctagtaaaa ggtatctcat
     1381 atcctatatt atctatacta ctttcatcta tataagcatc ttcaacatga ggttttacct
     1441 ctttttcaaa atactcatac acatcttctt taagtgctat actttcagta tcttttaaac
     1501 tactatcact ctctatattc cctttagcat ctttgcaaac tactgcgtct tcatttctta
     1561 ctcctatagc attaactata gctttaatta gtcctgctga taaagctaca tcatataact
     1621 tagacttaga ttttaaatct ttaataaact tatccctctt catatactca aattcactat
     1681 caaaactttc taaaagtttt aataacttat cttgttttac tcttccttct gcttcttcct
     1741 taactttaac ttcttcatct tttttcttag aaactgaaag atttataaat tgagtagtat
     1801 ttttaacatt ttctatagct tcttcattta ctctaaagct cagttttaaa ggtctttcta
     1861 ttgttacttt tctatatcca aagccttcat tgtcaaatat tttactattt tcactttctt
     1921 caaatctttc atatatttct tttatttctt ctatttgttc aagacttatt tcttttcttt
     1981 tatttcctaa actttttctc attaattgat aatattcact agcatttaca agttgtatct
     2041 taccttttct tttatcctct tttttattag taagtaccca tatatatgta gctattcctg
     2101 tgttatagaa catatcagta ggtagtgcta ttataccctc tagtaaatcc ttttctatta
     2161 tccattttct aatgttagat tcaccacttc ctgcatctcc tgtaaataat ggtgatccat
     2221 tgaatattat agctattcta ctaccttctt catcatcata cattttgctt atcatatttt
     2281 gtaagaatag taacgaccca tcacttactc ttggtgttcc tgccccaaat cttccatcaa
     2341 atcctaagtc agcttcttct tttactttct tctcttcatc tttccatgtg acaccaaatg
     2401 gtggatttga tattaaaaat cttatctttt ctccactaaa tctatcttgc gtaagagtat
     2461 ttcctagtct tatattatct ggattttgtc ctttcattat catatctgcc tgacatattg
     2521 caaatgattc atctagtaat tcttgtccat atacatttac tatggcactt ggatttactt
     2581 tttccacata gtcttcagct attgaaagca tcccaccagt accacagcaa aaatctgcta
     2641 tagatattac ttttccttct tctgtaagga agttttcttt gcccataaat aacatttcca
     2701 tacaaagtct tataacttct cttggagtat attgttctcc tgctgctgag ttttctgtaa
     2761 atcttcttag catttcttcg tatatgtatc ccatttcgtt gtttgataca gagtttatat
     2821 gtaggtctac ttcatacatt ttgctaagga ctgcatatag cttgtctttt ttatctaatt
     2881 gagctatttc atctttgaat ttaaatttcc ctattatttc ttttacactg tttgagaatc
     2941 catttaggta ttcttcaaag ttcgatttta tattttcact atcatctaac agttttttcc
     3001 atgtaaaatc actcacattg tagaactgta agtctatatt atggttttct tttagatcca
     3061 ctattacaag ttcgtctttt tcttcttctg gtaagaaatc atattctttg tatactgatt
     3121 tcactatttc tctatcataa tcatctaata gacaatcaaa tcttcttatt actattagtg
     3181 gtaacattac ttttctgtag tcttcttctt tgtatgtacc ccttaaactt tctgctatat
     3241 tccataaaaa agcaatatga ttaaaattat tatctatact cacttatact atctcctttt
     3301 aattaaattt taaattagtt tatattataa ttatatcatt tcatggcaca aaaaaaacat
     3361 attgacaaaa ctatcaatat gtttttcgtg gatttcaatg gtggagacga ggggatgcta
     3421 cattcttatt tctgcatctt catgtcttgt cactttttat gttatatcaa cacttgtaat
     3481 ttttatattt catgtatatt at
//
LOCUS       DAGSHT010000280         3441 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.286, whole genome shotgun sequence.
ACCESSION   DAGSHT010000280 DAGSHT010000000
VERSION     DAGSHT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.286"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            153..1736
                     /locus_tag="MJB72_003477"
     CDS             153..1736
                     /locus_tag="MJB72_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8264536.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSIETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLFLSFVATPISTIIFKILSTIIAYIS
                     IKFIFLFLFQGSGYKFRKITDVLLLITCISSIIGAFISDRMYACMYGIQFVVSIISFF
                     YIIFYHWKRVAFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            1708..2328
                     /locus_tag="MJB72_003478"
     CDS             1708..2328
                     /locus_tag="MJB72_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264537.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(2513..3439)
                     /locus_tag="MJB72_003479"
     CDS             complement(2513..3439)
                     /locus_tag="MJB72_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBY8264538.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNKKTMKYKHRDELFKSNNINTYSRINEESIDKD
                     YDSIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYESPESIHETI
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTAMEIYIEP
                     TILFGYEFESYLSEESSLSACNYGDMCYEDMY"
BASE COUNT         1226 a          493 c          411 g         1311 t
ORIGIN      
        1 ggtttgatat atttataaaa ttgatataat tcaaagttac taatccttaa atttaattca
       61 atcataaaat tggtataatt caaagttatt aattcttaag tttgattcag tcataaaatt
      121 aatataatta actcataagg aggtgtataa gattgacaaa ttttaattta aaaatactat
      181 ttttaggaaa ctacattcta tcacttctct tcatgggaat atccacaagc attgaaactt
      241 atatgaatat ccaaagcatt atccccttaa taatctcaac attattactt atagatatta
      301 catatataac ctcttgtaat gtccgagaaa ataacattat ttcattgttt tgcggattgc
      361 tagcactaga tagttggtat ttattcttat catttgtagc cacaccaata agcactatta
      421 tttttaaaat attaagcaca attatagcgt atatctctat taaatttata tttttatttt
      481 tgtttcaagg tagtggatac aagttccgta agataacaga tgttttatta cttatcacct
      541 gcattagttc aattataggg gcatttatct ctgacagaat gtatgcttgt atgtatggaa
      601 tacagtttgt tgttagtata ataagctttt tctatattat tttttatcat tggaaaagag
      661 tcgcctttgt cataaaaagc gaaaaaaaac ctatatcgat ttcagtattt ataacaatta
      721 taggtttcct tacttattat ttttttacac taagaatatc aaatcatatt ggtaattttg
      781 gaatctatct aactgtattg gtatttttta tgagtattca tggtattgca cttaaagaaa
      841 atgatagttt tcctatttca acagttttta acataaaaca aagggctata tttcttttta
      901 taagtatttt agtaattagc ttggtcgtac tatttgccaa tgggaatttt tctataatat
      961 taatagctat aaatgtttta tttatgctta ttttcctctg taacattatt ttggagttta
     1021 accttagaga tgaaaaaaat aaagtagtta aaaatagcaa gtatactttt gcattagaaa
     1081 aattacaaca agaagaacaa ctaaaaacag agttttctaa ttttttacat gatgatatat
     1141 tacagaacct gctttcaata aaaaacatga tgagtaagtc atatcgccca gaagttcaag
     1201 atattatata tgaaacactt aacaatctaa atacatgtat aagaaagcaa atgcaagact
     1261 atcatcctgt tattttaaaa aatttaacag taaaagaaaa ttatcagaat ttaattgaaa
     1321 gtgtttcttc atcctttcca caaaaaaaca tttgtgtttc atttaagtgt tctgatactt
     1381 tattcttagt tgaaccatat gatatattga tatatcgact gattaaagaa ttattgacaa
     1441 acgtgtataa acattcaaat ggtagccatg catggatagc tttagcacta gaaaaaaata
     1501 taataaaatt aaatattaat gataatggtg tgaaaaatac atcttttaaa acacctttag
     1561 atataactaa aattactttt gatacaataa aacacaaagg gattatatca attaaagaac
     1621 aagtagaaaa tttaggagga acgataagta tttctgataa tatacctcat ggagtttgtg
     1681 tacagattac aatccctatg aaaggagatg gttcttataa atattttgtt agttgatgac
     1741 catatgcttt ttgctaaaag cttgtcaatt gcactagaag aatacgaaga aattgaacag
     1801 ttttattcca cacaggacat ccataatctt tcagaaatga ttcaagataa aaaaatagac
     1861 attgtcttaa tggatataaa ccttagaggc ttaagtgaat gtgatggctt aaaagtagct
     1921 tataatttat tagagactaa ttcaactatt aaaataataa tcttgactgg atacgacctc
     1981 ccagtttata aacatgaggc taaaaaaata ggtatctatg gattcctaaa taagaatatt
     2041 agccctgata gtttaattga ttcattatta aaagtctatc agggggtaac ttgttttcca
     2101 tctgaaaata ctgaagtaat tattgaagat ttaactaata tggagaaaag aattttacag
     2161 cttatctgta gtggtaaaaa aagaaaaata attgctgatg aattgtatat aagtgaaaga
     2221 acattatcaa atcatttaca acacatatat gacaagctag acgtttcatc atcagttgag
     2281 gctattacaa aagctattaa aatgggttat atttctcctg tgtattaaaa aaagtgggtg
     2341 tctcaaaatg aactttcgct catgaggcac tctcctttgt attaaatcaa atatattttc
     2401 atcatttaaa caattaagaa gaggtttctc tctattttga gacaactcca ttttaataca
     2461 ctatttatat ttatatataa gcattttaaa caactaaaaa acaactaata tcttaataca
     2521 tatcctcata acacatatca ccataattac aagcagataa agaactctcc tcacttaaat
     2581 acgactcaaa ttcataccca aacagaattg ttggttctat atatatttcc atagctgtat
     2641 atctccatct tcttaccttc gcctctataa ccttgtaaaa atcttctaaa ctataccctt
     2701 cttttaatct tttatctata agtgccttag ttatacttaa atcagcttta aaattttttc
     2761 ctgattcact atttaatttt cttataatag tttcatgtat actttctgga ctttcataag
     2821 tcgaatcttc agtcaaatct tcatccgaat tttcaaaggt atttttattt aaattctttt
     2881 tatttaaatt attttttaat aattcttttt tctttgtctc attttctgtt tcatacccag
     2941 tatgaattat cgaatcataa tctttgtcta tactctcttc atttatacgg ctgtatgtgt
     3001 tgatattatt gctcttgaac aactcatctc tatgtttata tttcattgtt tttttgttat
     3061 tgttaatggt tgcatcaatg tatttctcta tatttttgaa aatatcacta ttctttgtta
     3121 caatctctgc attactattt tcagaacaaa cataactata tacagaacaa caatttccct
     3181 ttactcccct acttattaat ctcaatatac ctaaatctat aaattcattt acaagtctac
     3241 ttattgtaga tatagataca tcaaggtcag atgcagcttt ccttactgtc atcataaatt
     3301 gtcctcttgg cagagttcct tttaatcttt gtatattttc ttttctcatt acatagtgat
     3361 taaaccttat tctatttaca tctctttttt cattaaaata taatactgct gaatttaatt
     3421 gataaaaact attactcata t
//
LOCUS       DAGSHT010000281         3392 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.287, whole genome shotgun sequence.
ACCESSION   DAGSHT010000281 DAGSHT010000000
VERSION     DAGSHT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.287"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..179)
                     /locus_tag="MJB72_003480"
     CDS             complement(<1..179)
                     /locus_tag="MJB72_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264539.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHV"
     gene            complement(182..850)
                     /locus_tag="MJB72_003481"
     CDS             complement(182..850)
                     /locus_tag="MJB72_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264540.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(1136..1663)
                     /locus_tag="MJB72_003482"
     CDS             complement(1136..1663)
                     /locus_tag="MJB72_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBY8264541.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(1880..3040)
                     /locus_tag="MJB72_003483"
     CDS             complement(1880..3040)
                     /locus_tag="MJB72_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264542.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSIFIENNSTNSAKITAKFSTRN"
     gene            complement(3028..>3392)
                     /locus_tag="MJB72_003484"
     CDS             complement(3028..>3392)
                     /locus_tag="MJB72_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264543.1"
                     /translation="LDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNN
                     QISLSKKEFDIVELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKH
                     TLENYIETVWGVGYKWIK"
BASE COUNT         1166 a          511 c          436 g         1279 t
ORIGIN      
        1 acatgtctat caccattaaa agaacgggct tgttcaattc ttaaattttg taggatacca
       61 attgactgtc caaccacagt tgtcataata gttgatagta ctattgctat aatgatgaat
      121 gtagtcataa gtttctggct ttttaattct tttattgaaa gtgaaaggta attcttcatt
      181 tctatttcac ctccataaga acaccatcaa taattgaaat ttttctatca gcaagttttg
      241 caattctatc atcatgagta attaccacta aagtctttcc aaactgtttt gaagttttta
      301 taagaagatt cataacttca ctgccacttt tactgtccaa gtttcctgta ggctcatcag
      361 caaatattat atctggtttt gcaataagtg ctctagcaat tgcaactctt tgttgctgac
      421 cgcctgaaag ttcatgggga agatgattga gtctatctgc aattccaagt aatgatgaaa
      481 tttcatttaa atagccttta tcaggttgtt ttttatctag aagtacaggc attgaaatat
      541 tttcagatgc tgtaagaaca ggtaaaagat taaattgttg aaagacgaat ccaaaggatt
      601 ttcttctaag tgcggatagt tctttttcag tgtgtttata tatatcttta tcatcaagta
      661 acacttgtcc agaggtagga ttatctaatc cacttaaaat atgtaaaagt gtgctttttc
      721 cacttccaga aggtccaatt acagaaacaa attcacctga gtttatttct aaatttacat
      781 tattaagtgc gataacttta ttgttattag aaccatatat ttttgataat tgttttgctt
      841 ttataatcat aaaaaacact ctccttttct atataagaag agtgtaagtt ttaaaactca
      901 attttttccc aacttttagc ttaaatctaa aaagtcttca ctatttgcgg ataattcgca
      961 gtgctgataa gcagtttcag tatcattttt aatttaaaat gtaatttatg cgctaaaggc
     1021 tttacaatac aactctattg ctttaaaact attttacaca taaacttgtg gataattttt
     1081 tatgttttac ttcctcatta ttatataaat tatttttgtc acaaattcta agttttcaca
     1141 taaatatact ttttatatca cgatttaatt cttttgattt tatcataatt gctatttctt
     1201 caaaagaaga aagaaaagcc acgatacata tcataattcc tgcaacatca agaccaaagc
     1261 aaatatagag taatggaaaa gcaaagatag aaattccagt gattttattc atcaaagtat
     1321 gaagtgatga aaatgtgcga tacttaaaaa aaccaatact gtaactaatc aaccgtaaca
     1381 atgctattgc aacaatacac caccatagat acatgggaag agcaatattt tttccgacag
     1441 taaccaaaat tgtaaccgca aaaatgaaat cagcaatact atctaacttt gcacctattc
     1501 cactttgttg tctcaacttt cgggcaataa gaccatcaag tatatcacta attccacacc
     1561 aaaaataaca tatccagaaa ggcagtgaac agaatgtaga actaagcatt agcaatgcaa
     1621 acacaatacg agagaatgta atattattag caaaactttt aatataatca cctccaaaat
     1681 tctaatttca tactattcta catatttaaa tatatatttc tattattgtt tcaaatgcct
     1741 tttgccaaaa tattataatt gaagcaactc ttacaacttc tacaaaatag tatatatatt
     1801 ggttataaat tgaaatacat tttataaatt ataaactaac aaagcttata taagaacagt
     1861 gaaagcttac aaaatcattt taatttcttg ttgagaattt agcagtaatt ttagcactgt
     1921 tagtagaatt attttcaata aaaatagagc caccatgttt ttcgcataat atttttgaga
     1981 tataaagacc cataccaaag tgtgcattgc tgtcatttaa agtcttatct ctatagaaag
     2041 gtcttaaagc taattttaga gattccttac taaatcctat accatcatct tcaattacaa
     2101 aggtaaggag ttcttgttca agatatataa gtatggatac attatttcta gcatatctaa
     2161 aagcattcga tattatattt tcgacgactc taagaatgat attttcatca aaaaatagat
     2221 ttatattatt tatttgactt ttaagtgtaa aagattttcc ttcttgttta gcaatgatag
     2281 atatattttc atctaagttt tctataaact tgtttatatt cgacatagat tttgaaacaa
     2341 ctaaatcttc taatcgttga gcattactca tattttctac atagtattca attctgttaa
     2401 tatgctcaga cataagttgt gtggttgata aaatcttctc atcactaagc ttacctgttg
     2461 gtatgtattt tgttaaatag tttgagtaac cttttagaac tgttagggga tttcttaaat
     2521 catgagaaaa agcagcatta agttgttttc tatcattaat catagtccac atctttatgt
     2581 tgtttttatt tagttcagac ctcatttttt caaaagcatt acatagatta cctaattcat
     2641 cattattatt atactctaag caaaagtcta aattgttgtt ggatatttcc attgcacctt
     2701 tattgagtat ttctatgggt gtttttaatt ttaccttgta aaaaattata tcagctaata
     2761 ataaaagtcc tataaaaaat ataagtggaa gtacaagctg aagtatgttg attatattaa
     2821 gtaatgtttt atcataatca ctaaattggt tcacattgtt ttgaactatg gttataatac
     2881 ctgtatttat aggtgttttt aaatacttat cttgtataga attatataga ttattaagta
     2941 aaatgtatgc gaaagtagta agcactgatg ctataattag tgaagataaa gtaagaacaa
     3001 agaaagcttt ttttaatgac atattattta ttttatccat ttataaccaa ctccccagac
     3061 tgtttctata taattttcaa gagtatgttt tgatagtttg attcgtattt ttctaatgtg
     3121 ttccattata acatcactat ttccattgct attgtatccc cagactctat catatatttt
     3181 ttctttatca aatacctgtc caggattcat agagagaagc tctactatat caaattcctt
     3241 ttttgaaagt gaaatctgat tattatatat acaaattaac cttttttcgt aatctattgt
     3301 taaatcatta taaaatttaa ttttagtttg atttgacttc ctttgttcac gtcttaaatg
     3361 ggcagatact ctagctccaa gttcgtctaa at
//
LOCUS       DAGSHT010000282         3387 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.288, whole genome shotgun sequence.
ACCESSION   DAGSHT010000282 DAGSHT010000000
VERSION     DAGSHT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.288"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..886)
                     /locus_tag="MJB72_003485"
     CDS             complement(<1..886)
                     /locus_tag="MJB72_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264544.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSE"
     gene            complement(1351..2286)
                     /locus_tag="MJB72_003486"
     CDS             complement(1351..2286)
                     /locus_tag="MJB72_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY8264545.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            2574..3140
                     /locus_tag="MJB72_003487"
     CDS             2574..3140
                     /locus_tag="MJB72_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8264546.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
BASE COUNT         1163 a          537 c          345 g         1342 t
ORIGIN      
        1 cttcactgag catttttttt atttctaaag aaagatttgc ataccccagc aacttatttt
       61 ttatatactc catttctctc tcaattgcat ttttattaaa atttctattc tccaattttt
      121 tctttaattc cttgtattga tttttgtata cattaattat tcccttacaa tccatgctta
      181 tattaaatac attactaaac aatttatttt gaagcaatat ttctgcttct tcaagtctat
      241 tttgctttaa atatatcata gctaaattaa cacttgtatc ccctattgac ttatgtatca
      301 tatttaaata caactctgcc ttttcataat cctccaacat acaatatgcg tttgataata
      361 taataagagc tgtttctttt atttggatat catctgtatt tctcactata tcttcaagaa
      421 tattcgttgt ttcttttact ctgtcattaa gttcactctt accaaattta tatgaataca
      481 tctgatacag cgaacatatc cttagcttca ataaatagct tttaggatac ttctctatat
      541 acatattgga aagctttttt acagattcta aatcttgtct aaattcttct tttggttctt
      601 ctgttgataa atcaatttca attctagcaa agcctgattc taattcttta taaattttca
      661 ttacttcttc ttcacttaaa tcaatcttgt aatttaaaag cttatcaata gacacattaa
      721 aaaagtctgc taataaaggc aataattcta tatctgggta agaatttcca ctctcccact
      781 tagatactgc tggtgttgac acacctataa actttgctaa ttgttcttga gttaaatttc
      841 gttcttttct aagacactgt atgactttac ctattttaat ttgcatatat acctccctag
      901 tacatctgtt gtaatttaag tatataaaaa aaatatttac tagtaaatcg agggaaagtt
      961 aacaaaatgt ctgtaattta accattggtt atattttaat ctaaaaccct atctcattgt
     1021 cttgaaatat aaaatctatt aattaagttt aagtataacc taattaatag attcacatta
     1081 aatagcattt aaatatttat attttctctt tactacaact tactatattt aaactttcaa
     1141 ggaattttaa ataaaataaa ttccatatac aatgataata atactgagtg ttaataattt
     1201 atttataata acacccacct attcaataaa tggcattata gcaaaaaatc acttctgggc
     1261 tctctctttt ctactttgca tatacataat ccacttttca aatattatat ataaataaat
     1321 ttaaagtaaa tatatcatta tattttttta ttacaatatt tctattttat tatttaacca
     1381 acaaattata tcctcaaaaa cttcttctct atttatttca ttcaacaatt catgccttcc
     1441 atctttgtaa agtttgcaag ttacatcttt tactcctaat tttatatatc tatcccttag
     1501 tcttaagact cctttaccat tttttcctat ggggtcttta tctccagata ttatgtaaat
     1561 aggtatatca agtggcactt tttttaagtt ttctttatcc tcaatttctt ttaacccttg
     1621 aactaaatca taaaaaaagc cacaactaca cactactcca caaaatgggt catttatata
     1681 tttttcaact tgttctttat ctctacttaa ccagtcaaac ttagtctttt catttcttct
     1741 tattatttcg ccgccaaata tcaaattatt tattttatta cttttagaac gtcttccata
     1801 ctttttaatc tcacgattga ttatcagatg agcaagattt agaatgattc cttgctttcc
     1861 atttgaacca catagtataa gaccagataa attattactg taatccatta tatatctttg
     1921 acttgcaaat gaacccatgc tgtgtccaaa tagataaatt ggcagatctt cattttcttt
     1981 ctttataata tttgttaaag tatacatgtc ctctacaaga catctaaatc cttctttttc
     2041 agctaaatgt ccaacatttt ctattatctt tgcagttttc ccatgacctc tatggtcatt
     2101 gatatatact atataaccat ttttagtaag taccttggca aaagtctcat acctttgtgc
     2161 agtttcagcc attccatgtg caatttggat aactgccttt ggttttttta tattttcatc
     2221 ttcccattta tatgtatata tatctaaacc ttcctctcct ttgaaagtaa agtttgtaca
     2281 cttcataatt atccctccag attgatttta ttcagtatat taatttatat attatattac
     2341 aagtattttc aagtcaaaat ctttctaaaa aatttaagaa ttattaattc ctcatagaag
     2401 ttatcattgg tacatagaag tttttacata aaattataca ataatttaat taattaatag
     2461 ttacataaaa aagtaatact ttaatatatt ttgacaccat attgacatta gaattaattc
     2521 atgatattct gttttcgaca aaacaacatc taaaaataaa ggagatatat ttcatggatt
     2581 attcaaatga actaaaagag ctctttttaa tgaatcaaac atatgcaact ctttttaccc
     2641 ttacaaacaa aatacaaata gaaggagata aatattttgg tatacttact tcaagacagt
     2701 atatgactat tttatcaatt cttcatttac cagaggaaga aacaacatta aataatattg
     2761 caagaaaaat gggtacaagt aaacaaaata taaatagact agttgctaat cttgaaaaaa
     2821 atggatatgt tgatgtaatt ccaagtcctc atgataagag agcaattaat gttaaagtta
     2881 cagacttagg aaaaaaggtt atggtgacat gtagtaggac tggaataaac tttatggcag
     2941 atgtatttca cgaatttacc aaagatgaat tagagacctt atggagttta ctaaaaaaaa
     3001 tgtatcgttt taatggtgaa gaacaagatg gctttgaaga agatgctaat tttatggaat
     3061 atgaagaaat agataaaatt aaatctgaag cacttgaaga atttgcaaaa agaagaaata
     3121 gagtaaataa aaaagattaa tactatcgca tctataatac cactttttta tagtacaata
     3181 ttttatataa ctataatgtc atttttattt tttaacaaaa caatcattac atagattagg
     3241 tgttcttagg aactcttttt ctgttttttg tacaaaaaaa ggagatgatt agacttttat
     3301 tttaaagcat ctcctttttc gctattatga ttattctact cctaaaatca tatcttttaa
     3361 tttttgtaaa tcattaattg gttgact
//
LOCUS       DAGSHT010000283         3374 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.289, whole genome shotgun sequence.
ACCESSION   DAGSHT010000283 DAGSHT010000000
VERSION     DAGSHT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.289"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..861)
                     /locus_tag="MJB72_003488"
     CDS             complement(<1..861)
                     /locus_tag="MJB72_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264547.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKIS"
     gene            complement(866..1603)
                     /gene="cas6"
                     /locus_tag="MJB72_003489"
     CDS             complement(866..1603)
                     /gene="cas6"
                     /locus_tag="MJB72_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBY8264548.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1861..2970
                     /gene="ugpC"
                     /locus_tag="MJB72_003490"
     CDS             1861..2970
                     /gene="ugpC"
                     /locus_tag="MJB72_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBY8264549.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            3000..>3374
                     /locus_tag="MJB72_003491"
     CDS             3000..>3374
                     /locus_tag="MJB72_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBY8264550.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELD"
BASE COUNT         1228 a          479 c          363 g         1304 t
ORIGIN      
        1 acttattttt tcatgcaaaa gttttttata aaatgattta tctgaataca tttttctttt
       61 aagcattatc ttatgattta aaaatgctaa ttctcttaaa tcattcgtat atgcaaaaac
      121 cattggtctt tttaaattta tatataaaag acacattaag ttgaaaaatt cacactcaaa
      181 acaaatatta ctttcaactt gtccacttct tttaaagcct gatttttctg cacctctaaa
      241 taaaaagtta tacttagagt cctttcgttc tcttaaatca atatctagtt ttgttgtttt
      301 atttatattg caaacattac atacactatc tttttcttca tattgtgtca aatcaagttt
      361 atctatttca tctatctcta tatttgattt aattacagta tctactattc tttttacatt
      421 tgtttccata ttttctaaac tcgtgtttaa tccatactta ccactatttc taacatatgg
      481 aaacctcctg tttttaaaat cttttttaat atcttccttt tgtttagcag ttgcagaaga
      541 tttatctaat tcttcaataa aatttacata cgttaaatta tctatattac taaatatttt
      601 ttctaaggtt tcctttttcc catttttaaa taccttaaaa agtcgatttt ctaatatata
      661 atcagatata tacttagggt ctatatattc tgcagattct aaagataaat aatttctatt
      721 taactcacaa gtaaatttta gcttataatc atttttttct agttcgtttt ttaaatccat
      781 tagtaattca taaaagtaaa caatgccaat atctcttaca atatcacctg atctataaaa
      841 tttagctttt atttctctca cctcgctact ttaatatatc aaccattcct gcattttctg
      901 tccttctata tccaattccc attttataca gtgcatttaa atcttccata tctcctttta
      961 aaaaaaatgt tcctgcataa gcatttatat agtaatattc tctcacttta aatccacgaa
     1021 tcttttcttt taacactctt tttttgaagt taataggtat aaattccaat ggctttctaa
     1081 gcccactacc tcttatactc tccagagtta gattagctat atagtttaaa cattctatat
     1141 aatttgaatc ttctacatct aaatatttac cttctttatt ctttactatt ataggagaca
     1201 tagttttaaa tataacacca ttttctttta tctcgtgttc tttatgtatt tttatatttt
     1261 ttattttaaa tctataacct ttatattcta gctcctttac ttttaaaatt ccattgtata
     1321 atttactgaa catagaataa ttataaaaac ttatattgaa tattatatct ccatcaacaa
     1381 gtaactcatt tttgcttctt ataagatttg gtatgtaaat ggctgtattt atatccttta
     1441 tttgcttatt gttttttctt tcttccccat agttaaatat gtttttagca aaatctttgt
     1501 tttctgcatt taaaatattt tttataaagg aaactgacaa cattgaattt tcatttggaa
     1561 atttgccatt acatgattcc ataaaaattg aaattctgct catattttca cctcctactt
     1621 tctagtataa ttcattattc tctatttgta atataaaaat aaaacatatc ataaaatgat
     1681 tttttgataa taaatttaat tttgtttttt aattttttaa atatattcta ctgttagttt
     1741 tattctttgc taaaaactaa atatacatat taaacaaata catttagtca ttttacacat
     1801 ttaatataat aaaaaaattt gatactatat aattaaaatt taaatatcaa ggaggatatt
     1861 atgtcagaag taattttaaa aaatatatca aaactttact caaatggatt taacgcagta
     1921 aaaaatataa atatagacat taaagataaa gaatttatcg ttttagtagg accatctggt
     1981 tgtggtaaat cgactacact tagaatgatt gctggtcttg aagaaatctc tgaaggagaa
     2041 ctatacattg gagataaact tgtcaataat atagaaccaa aagatagaga tatagctatg
     2101 gtttttcaaa actatgcttt atatccccac ttatctgttt acgaaaacat ggcatttgca
     2161 cttaaactta gaaatttgcc taaagatgaa atagacaaaa aagttaaaga agctgctaaa
     2221 atattagact tactacctct tttaaataaa aaacctaaaa cattatctgg aggacaaaga
     2281 caaagggttg ccttaggacg tgcaatagtt agaaatccta aggtattctt aatggatgaa
     2341 cctctatcca acttagatgc aaaacttaga actgctatga gaacagagat aactaagcta
     2401 caccaacagc ttggaactac atttatctat gtaacacacg accaagttga agctatgact
     2461 atggctgata gaatcgtagt aatgaaggat ggagtagttc agcagattgc aactccacaa
     2521 gatgtatacg attatccagc taatatattt gttgcaggat ttattggagc accacagatg
     2581 aattttattg atgtaatatt aatagaagaa aatgatgaaa tttatgcaca aaatgaacat
     2641 attaagctta aattgaataa agaaaaacac tatgatttaa taaaagacaa ttatattaat
     2701 aatgaagttg ttataggtat cagaccagaa gatattcatg tagaagatac ttttattaaa
     2761 tctaatccag atacttgttt taaatcaaga attgaaataa aagaacttat gggagcagaa
     2821 acttatgctc acttaaaact aggcgaaaat acgataacaa ttagatttga cagtaaaaac
     2881 agaattaatg ttggtgatga cttaacactt tcagttgaca atagtagagt acacatattt
     2941 gacaaagaaa ctactttagc tataagataa gaatataatc ttatttttaa ggagacttta
     3001 tggaagagtt aaaagatttt ttagaaagac agcttaataa aaaaataaat atctatccat
     3061 atgaaaacca aaagttagat gctagtagcc atttagttac tttcaataat gctatttatg
     3121 ttattgattt tctagataaa aataatctcg ataatttaaa aggcaacttt tatttaattt
     3181 accaatctca tatagacttt gaatatctaa aaaatatttt ttataattta tttgaagata
     3241 taaatataat acaacacaat ggttttttta tagtaaattc taaatataac ttagatatta
     3301 atgtaactac acaaaatata attgaaactg aaacttatca aagtacttat attttttatc
     3361 ttggtgaatt agat
//
LOCUS       DAGSHT010000284         3371 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.290, whole genome shotgun sequence.
ACCESSION   DAGSHT010000284 DAGSHT010000000
VERSION     DAGSHT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.290"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            325..2967
                     /gene="adhE"
                     /locus_tag="MJB72_003492"
                     /gene_synonym="adhC"
     CDS             325..2967
                     /gene="adhE"
                     /locus_tag="MJB72_003492"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBY8264551.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAYFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1301 a          418 c          635 g         1017 t
ORIGIN      
        1 ataaaattta ataagtaaaa aaaatgaaag atataaatta tcattttagt attttattta
       61 gtcgaagtaa attattcaaa tgaaatatat ctcatttaga taaaaaattt tagtaaaagt
      121 aaaaaaaata atatatttgt taaaaaataa attgacaaaa tattcagaaa aaaacttctt
      181 gacctaagag agtaaaataa ataaaatcta aggtatagag atgaaatcta taaaattatt
      241 ttaagtataa gttaaaaata tagatgatgc atttaagatt tataaaaatt agagatgcat
      301 aattatttta gggaggtttt tagtatggag aaaaaagaaa aggtagtaga aaaatcaaat
      361 gttgaagtat gtagtcctga aattgtcaac agtgttgaaa cattaagaat gagattagaa
      421 gaaattagat tggcacaaaa agaatttgca acatttacgc aagaacaggt agataagatt
      481 ttcttagctg cttcaactgc agctaatcaa caaagaatac ctctagcaaa aatggcagtg
      541 gaagagactg gtatgggtat tgttgaagat aaggttataa aaaaccattt tgcctcagaa
      601 tatatctata atgcttataa agatactaaa acttgtggtg tcatagaaaa agatgaagca
      661 tttggtttta caagaattgc tgagccagta ggtgttttat cagcagttat accaacaact
      721 aatcctacat caactgcaat attcaaatcc ctaatagcac taaaaactag aaatggtata
      781 attttctctc ctcatccaag agctaaaaac tgtactatag aagcagctag agttgtacat
      841 gatgcagcag ttaaggcagg agcaccaaaa ggacttatag gttgggtaga tgttccttcg
      901 attgaattaa ctaatgttgt catggcagag gcagacctta tattagctac tggtggacct
      961 ggaatggtta aatctgcata ctcttcaggt aaaccagctg ttggagttgg gcctggaaat
     1021 gtacctgcaa taatagatga gagtgcagat ataaaaatgg ctgtaagttc aatcttagta
     1081 tcaaaatcat tcgataatgg tatgatatgt gcttccgagc aagctgtaat agtaccagaa
     1141 aaaatatatg aagaagttaa aaaagaattt aaatatcgtg gagcatattt cctaaataaa
     1201 gaagaaactg aaaaagtagg aaaagtagta ataattgatg gttcattaaa tgccagaata
     1261 gttggacaac cagcacatgt aatagccaaa atggcggatg tagaagttcc aaaaacagct
     1321 agaataataa ttggtgaagt agaatctgtt gaattaaatg aaccttttgc acatgaaaaa
     1381 ctttctcctg tacttgcaat gtacaagtct aaaagttttg aagatgcagt tgctaaagca
     1441 gaaaaattag ttgcagatgg aggctatggt catacatctt cattatatgc agactctata
     1501 aatcatccag agagagtaga aaagtttgta aatgctatga agacttgtag agtattagtt
     1561 aatactccat catcacaagg tggtataggt gatttatata actttaaatt ggcaccgtct
     1621 cttactcttg gttgtggttc ttggggtgga aactcagtat ctgaaaacgt tggagtaaaa
     1681 catttgctta atatcaagac agtagctgaa aggagagaga atatgctttg gttcagagca
     1741 ccagaaaagg tttatttcaa aaaaggatgt ttaggtgtag cagcaagaga atttaaggat
     1801 gttatggata aaaagaaagc atttatagta acagattctt tcctatataa taatggatat
     1861 actaagaaat taactgattt attagatgag atgggaatta agcatactac attctttgat
     1921 gtagctccag acccaacatt agcttgtgca agagaaggag ctaaagctat ggctgatttc
     1981 caaccagatt taataatagc tgtaggtgga ggaagtgcaa tggatgctgg taagataatg
     2041 tgggtaatgt atgagcatcc agaagtagat ttccaagact tagctatgag atttatggat
     2101 ataagaaaaa gagtatatgt atttcctaag atgggtgaaa aagcgtattt tgctgctatc
     2161 ccaacttcag caggaactgg ttcagaagta actccatttg cagttataac tgaccaagat
     2221 tcaggtgtaa aatacccatt agcagattat gaattaatgc caaacatggc tataattgat
     2281 gctgatatga tgatggagat gccaccaaga ttaacagcag catcaggtgt tgatgcttta
     2341 acacatgcat tagaagcata tgtatctatg ttaagaactg aaccagctga cggtctagca
     2401 ctacaagcag gtaaaataat atttgaatat ttacctcgtg catataaaaa tggaaagaat
     2461 gataaagaag caagagaaaa aatggcaatg gcatctacta tggcaggaat gtcttttgca
     2521 aatgccttct taggaatatg tcactcttta gcacataaat taggtgcatt ccaccatgtt
     2581 caacacggtg ttgcaaatgc attattaata aatgaagtaa tcaagttcaa ttgtgcagaa
     2641 gctccaaata agatgggtgc attctcacaa tacagatatc ctgattgcat acaaagatat
     2701 gctgaatttg cttcttttgc aggtattaaa ggaagtacag accaagaaaa agtagataat
     2761 cttataaaag caatagatga actaaaggca aaagtaggtc ttcctaagac tataaaagag
     2821 gctggagtag aagaatctaa attcttagaa agattggatg ctatggttga acaagctttt
     2881 gatgaccaat gtacaggtgc taatcctaga tacccattga tgagtgaatt aaaagagata
     2941 tatctaaaag tttattatgg taaataatat ctattgtgca acataggttc tattgggttt
     3001 gtatataaat tattagattt gaatataaat agagttttat tagagacacg tagaagtaac
     3061 tataaataat agattataac ttaaattcta agtggaaatg ataaaaagtc agtaatttaa
     3121 tggtaatttc gaatatgtgt atttaatcaa ttaactttaa aattagtcgg aaaatttttt
     3181 tattacatat aattttaaag cataatatat aaaaaacttt atgaaaaaat tataaaaata
     3241 ttcagaaaat tctattgact aatttattta tcatgatata ataagtttag aaaagaaaaa
     3301 aattggggcc tattaaggcc cccttactat tttttgatag atttatttaa aattactttc
     3361 tcattttgat t
//
LOCUS       DAGSHT010000285         3264 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.291, whole genome shotgun sequence.
ACCESSION   DAGSHT010000285 DAGSHT010000000
VERSION     DAGSHT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.291"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            283..2694
                     /locus_tag="MJB72_003493"
     CDS             283..2694
                     /locus_tag="MJB72_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY8264552.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            2991..>3264
                     /locus_tag="MJB72_003494"
     CDS             2991..>3264
                     /locus_tag="MJB72_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY8264553.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPSVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDD"
BASE COUNT         1309 a          408 c          564 g          983 t
ORIGIN      
        1 tgtaaaaaat aggtaagtaa taatgtttat taaaactgtt aaagcaccaa aaaaaagata
       61 taatattgtt tctagatgtt tttttaatat caaaaaattt tcctccatta tttggtatat
      121 attattatgt aattagtata ccacatatta atcaataaat atgcagtata aaatagtatt
      181 ttatatatgt ataattataa ttcctatttt tataaaaata aaatatgacc tagatttaga
      241 aatctgatat aataaaaatt aaatacatac aagggggtaa atatgagaaa gtataaatta
      301 aaaaaattgt caaagctact ggtgttgttg acagtttgtt ttttaattgt gtcaacaata
      361 cctgtctcag cagaaaaaaa taaaattctg actggagtag aaactgcaga gtattctgaa
      421 tcatatcttc aatacttgga agatgtaaaa aatggagata tagcaaaata taatggagta
      481 ataccaactc cttatgaaat ggaaggaaca acacttaaaa ctaatgtaag aagtagtctt
      541 ccagcatcat ataaatcaag tgtatcatat gatccaagaa aattagattt aactacacca
      601 gcaaaagacc aaggaaaact taatacatgt tgggcttttt ctggaatgtc aacactagaa
      661 gcatatttga aattaaaagg ttatggaact tatgatcttt cagaagagca ttttagatgg
      721 tggtcaactg gaggaataca tggatggaac ttaactgata tgacaggtag ttcgaatgta
      781 actgcaattg gatatcttac agcctgggca ggacctaagc tggaaaaaga cattccatat
      841 aattttaaat cagaagatga aggagcaact aggcctcaaa atatggatac tgcacctaca
      901 caatttaatg taacagatgt tgtaagaatt agtaaagata aaacttctgt taaaaatgca
      961 ataatgcaat atggtgcagt gacatcagga tatgcacact attcagccta cttaagtgat
     1021 gatgaaaatt cgtataattg taatgataaa agtgaacctt taaatcattc tgtttcaatt
     1081 gtaggatggg atgataatta ttcaaaagat aagttcaaac caagtgttag accagaatca
     1141 aatggagcat ggttagtaaa aagtagttgg ggagaattta actcagaaaa aggctttttc
     1201 tggatttctt atgaagacaa aactctttta aaagatacag ataactacgc aatgaaatca
     1261 gtatcaaaac cagatagcga taaaaaaatg taccaacttg aatatgctgg tcttagcaag
     1321 ataatgtcaa ataaagtaac agcagcaaat gtatttgatt ttagcagaga ttctgaaaaa
     1381 cttgactctg ttatgtttga aacagattct gtaggagcaa aatatgaagt atattatgca
     1441 ccagtagtaa atggagttcc tcaaaacaat tcaatgacaa aacttgcaag tggaacagta
     1501 tcatattctg gatacataaa tgtacctact aattcttaca gcttaccaaa aggtaaagga
     1561 gcaatagtag tagttataga caacacagca aatcctaata gagaaaaatc aactttagca
     1621 tatgaaactg atatagatgg atactatcta tatgaggcta aagcaaactt aggtgaaagc
     1681 tacatacttc aaaacaataa atttgaagat ataaatacat atagtgaatt ttctccttgt
     1741 aactttgtta taaaagctat aacaaaaaca tcttctggac aagctacttc aggagaatct
     1801 ttaactggag cagatagata tgaaacagca gttaaagtta gtcaaaaagg atggacttct
     1861 tcacaaaatg cagtattagt aaatggagat gcaatagtgg atgctttaac agctacacca
     1921 tttacagcag caattgactc tccaattctt ttgacaggaa aagataactt agattcaaaa
     1981 actaaggcag agttacaaag attaggaact aaaaaagttt atctaatagg tggagaaaat
     2041 tctttaagca agaatgtaca aactcaactt agtaatatgg gtatatcagt agaaagaatt
     2101 tcaggtagtg atagatacaa gaccagtata tctctagctc aaaagttaaa tagtataaaa
     2161 tctgtttcac aagttgcagt ggcaaatggt gtaaatggac ttgcagatgc aataagtgtt
     2221 ggtgcagcag ctgctgataa caatatgcca ataatactta ctaatgaaaa gagtgagttg
     2281 caaggtgctg atgagttttt aaattcatct aaaataacta agtcttatat aattggtgga
     2341 acagctactt tatcatcaaa tttagaaagt aagctttcaa atccaacaag acttgcagga
     2401 agtaatagaa atgaaactaa tgctaagata atagataaat tctatcctag ctcagatttg
     2461 aaatatgctt ttgttgttaa agatggttca aagagtcaag gagacttgat agatggacta
     2521 gcagtaggag cattaggtgc taaaactgat tcaccagtag ttttagttgg aaataagtta
     2581 gatgaaagtc aaaaaaatgt acttaagtct aagaaaatag aaactcctat tagagttggt
     2641 ggaaatggaa atgaaaatgc ttttaatgaa ctaaatactc ttttaggaaa atagtgaatg
     2701 gaaagatagt aaggatatac attttattat taaaaaatat tcaatagaag tttataaata
     2761 taaaataaca aaacggggtg tctcaaaatg aactttttag ttcatgaggc actctttttt
     2821 gttgttttat atttttatca tttaaataat gaaaaatagg attgtctata tttttagata
     2881 gttttcttat tattgatgaa aaatgtaata attttaaact gtaaaatagt gtaaagttat
     2941 tttataatat aaatatagta agattttaaa caaaataagg gggaataata atgaaagcac
     3001 caaaaactat tttaacaata ctgacaatag cgcttacttt aagtagcatt tctataatac
     3061 catcatatgc acttacagag gaaaaattaa taggtaatgg tagatatgaa actgctgtaa
     3121 agataagtca aaaggcatac agttctagtc ctagtgtagt gctagttaat gacaattcat
     3181 tagctgatgc actttcagct acaccatttg caaaggctaa aggagcacca atacttttga
     3241 cagaaagtga taagctagat gata
//
LOCUS       DAGSHT010000286         3154 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.292, whole genome shotgun sequence.
ACCESSION   DAGSHT010000286 DAGSHT010000000
VERSION     DAGSHT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.292"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..511
                     /locus_tag="MJB72_003495"
     CDS             <1..511
                     /locus_tag="MJB72_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264554.1"
                     /translation="YSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLD
                     YMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLL
                     DDKIYSISGIKNSISGELKGFKGVVDVVGTKPVKFQVFDMKLKKNIYEAELINGDISL
                     QEKLFFVAN"
     gene            877..2058
                     /locus_tag="MJB72_003496"
     CDS             877..2058
                     /locus_tag="MJB72_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY8264555.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEAVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(2200..3117)
                     /locus_tag="MJB72_003497"
     CDS             complement(2200..3117)
                     /locus_tag="MJB72_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264556.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         1217 a          365 c          518 g         1054 t
ORIGIN      
        1 ttactctaac tttattatat atgatataga agataacact tttaatataa gtaataaatt
       61 tatggataat ctaaacaaag aaaagaatgc aaatccaaag gagattggct tattaagtaa
      121 aactaagcta gattatatgg taaaaaataa taaattgaat cttatagtta cggatatgag
      181 taattccata actaatcttg tttatgatat taattctaat gagattaaat tagaagatta
      241 tgagatactt gatttaaaag tagattataa tgaagcatca tctggagtta ggaaagttaa
      301 gctactggat gataagatat attctatttc aggtattaag aacagtatat caggagaatt
      361 aaaaggattt aaaggggttg ttgatgtagt cggaactaag ccagtaaaat ttcaagtatt
      421 tgatatgaaa cttaaaaaga atatttatga agctgagcta ataaatggag atataagttt
      481 acaagaaaag ttattttttg ttgcaaatta agtgttagaa ttaattttag aactttaaac
      541 ttttatatgt atcacagata agtataagta agggagtgtc tcaaaataaa ctttttagct
      601 catgagacac tttttttata tgaaatcaaa tatattttca ttgtttagat ttattttgat
      661 aatgaaaaaa agattatctt tattttaaga aagacattta tttataatta tatacaaaaa
      721 tattttttaa tgtttttgtg tataattata acaatttaat ttatattaat gcacttaaat
      781 ttgaaataga atgcttatag tggtaaaatt aattattaga atattagaaa taataaataa
      841 ataatacatt gtacttaaaa gggggagaaa tttaagatga acaaaaaata tattaaagca
      901 gggaatttaa ttgatggaac cggaaatagt attatgcaaa ataagggtat aattattgat
      961 ggagatacta tagttgaaat tgaagatata agagatgatt taaatggata tgatgtgtac
     1021 gattacttag ataaaactgt tatgccaggc attatgaatt gtcatgttca cttgacaatg
     1081 gaggcagttg gaaatccaaa gatatattat gataatgtat cagatgtaga gcttgtagta
     1141 aatacgataa aacaacttga tgcttatttg gattcaggag taacatatat tagaagctta
     1201 ggttgtccca aatatgtgga tgtacaactt aaaaatctta tagacaaagg gtctatagat
     1261 ggtcctggta tagttacttc aggtccagtt atatgtatga caggagggca tggatattat
     1321 tttggtattg aaagtgatgg agtggatgaa tgtagaaaat cagccagaac agttttaaaa
     1381 aatggagtag attgtgtaaa gataatggca actggaggtg taacaactga tggagttgag
     1441 ccagggtcac cacaattaac tttggaggaa atgaaggctg ctgtagatga agctataaaa
     1501 gcaggtaaaa taactgccac acatgcacaa ggaagaactg gtataaaaaa tgcagtgctt
     1561 gcaggaatta cttctataga acatggtgtt tatttagatg atgaaataat tgatttgatg
     1621 cttgaaagag gaacttactt agttccaact gtggctgcac catattttat tctaaatgca
     1681 ggaaaagaaa gtggaataac tgaagctact cttagaaagt gtgagattgt tgccattcca
     1741 cacaaagagt cattcctaaa agcatataaa aaaggaatta aaattgcagt tggttctgat
     1801 gcaggaactt cttataatga acatggaaaa acatattatg aaatgaaact aatgtcagat
     1861 tatggtatgg ataatatgga tataatagtt gctgctacaa aaacagcatc tgaactttta
     1921 agaattgata aaaattatgg aactctagaa aaaggaaaga aagcagatgt tatagttgta
     1981 aatggaaatc cagtagagaa tatagacgta cttgcagatg tgttagcagt atttaaactt
     2041 ggtaaaaaag ttagataaat agtataaaaa taaaaaagag tgtctaaaga aatttttaaa
     2101 taattataga cactcttttt tattagtaat aattaaatct tttatacttt agaaaataga
     2161 ttctatttta tatttagata taactcttta aaatcattct tatgcaatat aatctataga
     2221 atctacacat acaaagtata ttcttgaatc attagaattt ccaagtacta taacatcatt
     2281 tttcttacct agaacttcaa caccagttag aaccaaacta ccagctacta cagtaacttg
     2341 attatttata tttgacatat aaagattatc taggatacct ttattacaac agcaatcatc
     2401 acaatcgcag ttacaattac attctttgta atgtttttta tcaagatagt aacctaaaga
     2461 tgctatgaaa tcttcataat ctgcaggtgt aatatcaaat gcaacagctt taagattaca
     2521 caatgatgct aaatctacgt ttgctaatgg agtagatgca gcaagtataa aatcaattat
     2581 agcagcaagt attgcatcta atatagcccc aaggtctatc gtaggaggaa taactgcatc
     2641 aataagtgca attaattcag ctactcctgg tatagttcct atagtattaa ttaatccctc
     2701 aagagttaaa gggactggaa taggataaga tactctacca gctatatcta ttaaatcaca
     2761 gttacaagca gaaaatcttt caaaagtacc atcaagtcca gataaattat cttttggagg
     2821 agctgaaaga tctataccta ccaaattagc acctactata aagaaatctg agataaaagc
     2881 aaattgatta aagtttacaa aaggtcttag agcatcatat cttagaagtt ctaaggcctc
     2941 tctcatactt tttttacaac aacattgttt tattttaggt tctttatatt tatttggata
     3001 attatagtat ctatcagcta cttgataatt ttcatagtat ctttcatttg tatttggata
     3061 atcttcctca tattcttgtg taaatctaaa gtcctctcta catttattat tttccactaa
     3121 atacactcct tttatatttt aatttaaatg tata
//
LOCUS       DAGSHT010000287         3151 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.293, whole genome shotgun sequence.
ACCESSION   DAGSHT010000287 DAGSHT010000000
VERSION     DAGSHT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.293"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            294..2429
                     /gene="rnr"
                     /locus_tag="MJB72_003498"
     CDS             294..2429
                     /gene="rnr"
                     /locus_tag="MJB72_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBY8264557.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKNTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1388 a          282 c          563 g          918 t
ORIGIN      
        1 ccaatcctat ttgatggtgt ataattaggt gataaagagt aatttaagca attatgataa
       61 agaataactt aagtaattat aatgatattt acaaataatt tataaaacat catgatttgt
      121 ttgaagttag tatgacaaag cttacattaa atcaaaaata taaataagta attttaactt
      181 ataataataa gaaaatatag aaaattatgt gataagtaaa aaatgttttg tctgaagaaa
      241 tataaaagac aatataaata ataatataat gaaaagtaaa acaggaggga aaaatgatac
      301 caggacttaa agaaagtttg ctaggattaa taaatgaaag tgcatataat cctctaaaaa
      361 aagaagaatt agcagaaata tttgatatac attcatcaga aatgccaatg ttttataatt
      421 ttttaaatga attagaagaa gatggttata tctgctttac taaaaaagga aaaatagtat
      481 ctcccaatca aatgggatac tttgttggaa aatttgtatc acacaaaaag ggttttggat
      541 ttgtagaatc tgatgttgaa tatacacagg atttatttat ctcaagtgac aatataaatg
      601 gtgctatgca taatgataga gttatggcag aaatagtagt acctgcaact gaagaaaaga
      661 gagcagaagg aagaatagta aaagtaataa agagagaaat aactagaata gtaggtacct
      721 tccaatcaag taaaactttt ggatttgtaa caccagacaa caagaaattt actaaagata
      781 tatatatacc taaaaaacat tttagtggag cagtagataa tgataaggta gtatgtgaaa
      841 ttacagtatg gccacaagag gatagaaaac cagaaggcaa aattatagag atactaggtc
      901 aaaaaggtga aagaggcgta gaaatagatt ctataataag agaacatggt cttccagaag
      961 aatttccaaa gaaagtattg caagaagcag aagctgtagc tgttgagatt cctcaagaag
     1021 agataaaaag aagaagagat ttaagagatt taaacatatt tactatagat ggagatgacg
     1081 caaaagactt agatgatgcc atatcaatag aagtattacc taatggaaac ttcaaactag
     1141 gagttcacat agctgatgtt acacattatg taagagagaa aaataaactt gacaaggaag
     1201 ccttaaaaag agctacatca gtttatttag tagataaagt aattccaatg ttgccaaaaa
     1261 ctctttcaaa tggagtatgt agcttaaatc catttgaaga taagctaact ctttctatat
     1321 ttatggaaat agaccataaa ggaaatgtgg ttaaacatga aatatgtgaa tctgtaataa
     1381 attcaaaagc tagaatgact tatacagaag tatctgatat attagaaaag gatgatgaaa
     1441 aacttaaaaa tacttttgaa catgttgttg aagattttaa aaatgctgaa attttagcta
     1501 ggattcttat gtcaagaagg gaaaaaagag gagctataga cttcaatttc ccagaggcaa
     1561 agataattct aaatggaaaa ggcgaagttg tagatataaa gccatatgaa agaagaatat
     1621 ctaataagat aatagaagaa tttatgctta taagtaatga gacaattgcc gaacattttt
     1681 actggatggg aataccgttt gtgtatagaa tacatgagac accatctttg gaaaaaatgg
     1741 aagaattgag taagtttata tctacatttg gatatataat aaaaggtgat aaagaggaag
     1801 tacatccaaa agcattacaa ggtataatag agaaaataaa gggcaaaaaa gaagaaggag
     1861 ctatcagtac tataatgctt cgttctttaa aacaagctaa gtactcacca gaatgtagtg
     1921 gacattttgg attagcagct aaattctact gtcactttac ttcaccaata agaagatatc
     1981 ctgaccttca aattcataga ataataaaag aatctttaaa taataaaata agtggaaaaa
     2041 gacaagaaca gcttactact atagtggatt atgcatctgt tcaatcatcc gagaaagaaa
     2101 gaaaggcaga gttagctgaa agagatgtac atgatttcta taaggctctt tatatgaagg
     2161 ataaagttgg tcaagaattt gagggtgttg tatctagcgt aacttcattt ggaatatttg
     2221 ttgagttaga taatactata gaaggattga taagacttgc aaatatgaat gatgattact
     2281 atatttatga tgagagttct tatagtgttt taggagaaag aactaagaag tcatataaga
     2341 taggagacct tgttaagata aaggttgaaa gtgttaatgt agattttaaa gaaattgact
     2401 ttgagatagt tgaaaaaata gaggagtaga taaaacagct aaaataagag ataacaagtt
     2461 gtagtattta tgatatgtat aaatatatag tgcacaatac tctataaaat aaatgataaa
     2521 aataggtaat tgagaaatat ctaatggaaa tagttaactg aaagataaat aattaagaaa
     2581 tatttaatga aagtagctaa ttgaaaaata agtaattgag aaatgtttaa tagaagtggc
     2641 taattgaaaa ataagtaatt aagaaatgct taatagaagt ggctaattga aaaataagta
     2701 attaagaaat atttaataga agtggctaat tgaaaaataa gtaattaaga aatgcttaat
     2761 agaagtggct aattgaaaaa taagtaattg agaaatgctt aatagaagtg gctaattgaa
     2821 aaataagtaa ttaagaaatg tttaatagaa gtggctaatt gaaaaataaa taattaagaa
     2881 atgtttaata gaagtgacta attgaaaaat aagtaattaa gaaatgctta atagaagtgg
     2941 ctaattgaaa aataagtaat tgagaaacat ttaatagaaa aagctagctg aaagataagt
     3001 aattaagaga tatttaatgt aaacagataa tttaaaaata cctaattaag aatagatatt
     3061 aaaaaatata atttcaatat aaaaataagt gtatcattta aaatttaaga tacacttatt
     3121 ttttatttaa gataactttt cttttttaga a
//
LOCUS       DAGSHT010000288         3044 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.294, whole genome shotgun sequence.
ACCESSION   DAGSHT010000288 DAGSHT010000000
VERSION     DAGSHT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.294"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..729
                     /locus_tag="MJB72_003499"
     CDS             <1..729
                     /locus_tag="MJB72_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264558.1"
                     /translation="EMKLLAIKENGLVLEYINNPTREMQELAIDNNIRAIKFIDNPTE
                     DMMIKAVNEGWSILDYIKNPTDKVIEMAINQAGWAIKYAKNPSEELQLLAVRKNYDSI
                     KFIKQPYESVQEEAVRISYDALRYIDSPSYNAELIAIKNNEAAISFITDLDKNKMLEF
                     LKVNILVIKYILKKVSKDELESVLKEVLSKEDVEEKYIRDFLNCSVIDRNSESIPIDK
                     IMFIYKYGSKTAKKIAVDEKLKIM"
     gene            741..1871
                     /locus_tag="MJB72_003500"
     CDS             741..1871
                     /locus_tag="MJB72_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY8264559.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1875..>3044
                     /locus_tag="MJB72_003501"
     CDS             1875..>3044
                     /locus_tag="MJB72_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264560.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANN"
BASE COUNT         1271 a          271 c          571 g          931 t
ORIGIN      
        1 gaaatgaaat tgttagctat taaagaaaat gggcttgtac tagaatatat aaataatcca
       61 accagagaga tgcaagaact agcaatagat aataatatta gagcaattaa atttatagat
      121 aatccaacag aagatatgat gataaaagca gtaaatgagg gatggagtat actggattat
      181 atcaaaaacc caactgataa agttattgaa atggctataa atcaagcagg atgggcaatt
      241 aaatatgcta aaaatccaag tgaagaatta caactgttag cagtaagaaa aaattatgat
      301 tcgataaagt ttataaaaca accttatgaa agtgtacaag aggaggcagt gagaataagt
      361 tatgatgcct tgagatatat agattcacct agttataatg cagaacttat agctataaaa
      421 aataatgaag cagctattag ctttataact gatttagata aaaataaaat gttggaattt
      481 ttaaaagtga atattttagt gattaagtac attctgaaaa aagtatctaa agatgaatta
      541 gagagtgtat taaaagaagt attatccaaa gaagatgttg aggaaaagta tattagagat
      601 tttttgaatt gtagtgttat agatagaaat agtgaaagta taccaataga taaaattatg
      661 tttatttata aatatggaag taagacagca aaaaaaatag ctgtagatga gaaattaaag
      721 attatgtagg agagagaaaa atgaatttta tagacacatt aaaaagcgtt gacttaatat
      781 tatccactgg agaagtacct ttaatagtag gtgaaagtgg aataggaaaa acagctttag
      841 caaataagct tgccaaagaa aatgattgga gtttaattgt tattgatgga aacttactta
      901 aagagggtga aataggtggt cttccaacta tagaatctta tgtaggagtt aattctaatg
      961 gatataagac tgaaaagaaa acaacagtgt atgctgttca taacaaatta agagaaattg
     1021 atgaagagat atctaaaagt aagacagttc ttttatttat agatgagata aatcgttgtg
     1081 agcatacagt acagcaagaa cttatgaatt taattttaaa tagagaaatt aatggatata
     1141 aactgcatga tgatgtaaag attttagcag caatgaatcc atcaagtaaa tatggttcag
     1201 attttgatta tcaagtggta gatatggatg cagcccaaga aaataggttt gtttggttaa
     1261 atatggagag tgaccataca caatggataa aatgggcaat agatgagggt attgaaagaa
     1321 aagttatcga gtttatttcc acttttccag aatatctgca taaaataaat gaagatgatg
     1381 taagagctac tcctagaagt tatgagagag tatctaaaat ttataaagtt tataaagaaa
     1441 aaaataattc tattcctaga gcagtatttt taaatgttgt aaagggtaat gtaggtaaag
     1501 ttattgcaga agaatttata agttttattg aatcaaactc tgaaccatta atatcctatg
     1561 aagatgtttt tttaggagaa tctattgatg agtctattgt agaaagagta aaaaatgaga
     1621 gtcatacaag actttatcta tcagctatga acattctaaa agacttagaa ttaaatatta
     1681 agaatgataa atatgaatca aaccactata ttaatagatt tattgaattt ttaaaaatgt
     1741 atcctgtaga tttaatgata ggtattatga aggatattag aaacagttat attgaagtat
     1801 ataaaaaagc tatagaaaat gaagagtttg taaaatcata ttttgaatct tatagtttaa
     1861 taaggggata atgtatggaa aattattttg ataaacaagc aaaagagctt tataataaag
     1921 caagtgaaat tataaatact cattctatgc taaaggcaaa tcgtgctaat gagaaatttg
     1981 atatagatat accacaagat tttaagtatg agttttttag tcttgtagat aaagtgaatt
     2041 taagtcttat ggaagaagaa gataattttt atggatattt tttgtttcag atgtcaagag
     2101 aaataagatt tgatataagt agtcctacag gtgtgaattt taagggagct aaatatgtta
     2161 tatattttaa tccaataatc tttttaacgc ttgacataaa gcaaatggag actacaatta
     2221 aacatgaaat tcatcatata ttatctatgc atttgatgag agcaaaggag ttaaaaggaa
     2281 agtatagtac attagcaatt aatatggcaa tggatatagt agtaaataaa tttctaaaca
     2341 atttaccacc atatgcaact accttagagt gggtaaattt aaagtattcc ttaaaacttg
     2401 aaccatatga accttttgaa tattatgtag aaaagattca gactgaatta gatttgatgg
     2461 aagtggatga agatggtgag gaagatgata gtgataaata tggagatata gaaattgatt
     2521 atagccccga aagaactcat gatatatggg atgaatccaa tgagatagat gaaaataccc
     2581 ttagagaatt tactgaaaaa tttattaaca gttctcaaaa aggtaaagtt cctgcttatt
     2641 tagaaagtat gatatcatca cttaaaaata gtaagggtga attgccatgg aatttatatc
     2701 ttaatagaat aatgggaaca gttgaaaata ataaaaagaa gactataaca agaagaaata
     2761 gaagacaacc aaatcgttta gatttgaggg gagaacttag aagtcataag gcagaaatag
     2821 cagttgcact tgatgtaagt ggaagtatta gtgatgaaga gtttaaacaa gctattaaag
     2881 aagttcttag tatagtgaaa aactataatc atgaaattac aattatagaa tgtgataatg
     2941 aaattagacg tgtatataaa gtgagaactg aaaaagatat aaaagataga attaggatag
     3001 gtggaggtac tagattttct ccagtctttg agtatgctaa taat
//
LOCUS       DAGSHT010000289         3038 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.295, whole genome shotgun sequence.
ACCESSION   DAGSHT010000289 DAGSHT010000000
VERSION     DAGSHT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.295"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..428
                     /locus_tag="MJB72_003502"
     CDS             <1..428
                     /locus_tag="MJB72_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264561.1"
                     /translation="KNTYSSKSSATISWYRWNGKEGADSSLIFTSQIYYNYKYNFKLF
                     IPNNLFDKILVEQEFVGEKAVFKFYYFDYSDRKNLFNIVVESKNKLEDRKNTGTQNSI
                     VLQESDEYTFLLVVNNANEMDKLDMTIDALKEYFSLIYG"
     gene            457..1155
                     /locus_tag="MJB72_003503"
     CDS             457..1155
                     /locus_tag="MJB72_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264562.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            1158..2591
                     /locus_tag="MJB72_003504"
     CDS             1158..2591
                     /locus_tag="MJB72_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8264563.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            2613..>3038
                     /locus_tag="MJB72_003505"
     CDS             2613..>3038
                     /locus_tag="MJB72_003505"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBY8264564.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMR"
BASE COUNT         1286 a          293 c          528 g          931 t
ORIGIN      
        1 ataaaaatac ttatagttct aaatcatctg caactataag ttggtataga tggaatggaa
       61 aagaaggtgc tgactcaagt ttaatattta caagtcagat atattataat tataaatata
      121 attttaaatt atttattcca aataatttat ttgataaaat acttgttgaa caggaatttg
      181 ttggagaaaa agcagtattt aaattttact attttgacta ttcagaccgt aagaatttat
      241 ttaatatagt agtagagagt aaaaataaac ttgaagatag aaaaaataca ggtactcaaa
      301 attctattgt acttcaagag agcgatgaat atactttctt actagttgta aacaatgcaa
      361 atgaaatgga taagctagat atgactatag acgcattaaa agaatatttc tcattaatat
      421 atggttaata ttatactcaa ttggaggttt tattttatga aggaaaagat acttatacta
      481 gaagatgaaa ttggtattag aagttttgtt agtataaact taaaaaggga aggctatgaa
      541 atagttgagg caggtactgg aagagaagct atagaaaaaa tgacaacaga aaaggatatt
      601 acaatagctc ttttagatgt aatgcttcca gatataagtg gtatagaagt atgtaaattt
      661 ataagggaga attttgacca agtaggtata ataatgctta ctgctaaagc tcaagaagat
      721 gataaaatag aaggatttat ctcaggagca gatgattaca tcataaaacc atttagtata
      781 aaagagcttt tagtcagagt ttctgctctt cttagaagag tagcaaagga tgattcaagt
      841 gttaagtcga gtgagatagt ttctccacca tttatacttg atatagacaa aagaaaatta
      901 tttaaaaatg gaaaggaaat tgagcttaca ccaactgaat tttcaatagt gaaatatcta
      961 atatcaaatg ctaagcagtc gttaagtaga gaccagatat tagacgaagt atggggaact
     1021 aactatttat atgattttaa aatagtagat gtaaatataa gaaggataag aaataaaata
     1081 gaagatgacc catcaaaacc aaaatatata caaactattt ggggttatgg atactgtttt
     1141 agaaaggaag aataatagtg ttacattttg aaggtctgag aaaaaaagta ataaaaaact
     1201 attttataat aattattata atggttactc tgtttgaagg attgttcatg ttttacatac
     1261 aaaactacta ttatgattct gtcaaacagc tacttgaatc tgaaataaaa tatgctgatg
     1321 aatataatgc tattacaatg gaaactacaa gttttgagaa aaaagtaaaa aatatatttg
     1381 ataaacaacc cttaactaaa aattcagaat ttggaatttc aataatagat aaagataaaa
     1441 atattatatt agaccaatat ggttttaaaa gtaaagaaaa agcaaactat gaagatgtta
     1501 acaatgcatt aaaagatata aaaactaaaa atttaacacc ttatacttat agaataccag
     1561 atacagggga gcatgttatg agtatctcct taccacttaa ggtaaataat ataatagaag
     1621 gtgttgttag atatacagta tcattagatg ctattgataa tgcaatatta aagcaagcta
     1681 catggcttat attagcagga atatttatac ttattatagc tatactaata agtcttaaat
     1741 ttgctgagac tcttataaaa ccattacgag agctaaagaa atttgcaaat gaattggctg
     1801 taggaaatta taatataaaa ttagaaaaga tgaagattgt agatgatgaa attggtgatt
     1861 tagcacagac atttgaacac atggcacatg agattgacaa aagtgaaaaa ttaaaagagg
     1921 aatttatatc atctgtatct catgaactaa ggactcctct tacatctata aaggggtgga
     1981 gtgaaacctt aggttatgaa agtataacaa gagaagaact agacttaggt ttaggcatta
     2041 tacaagatga aacagaaaga cttataaaac tagttgaaga attattagac ttttcaaggc
     2101 tttcttcaga caggataaaa cttcatgttg atatagttga tgttgaaggt ttaattgtag
     2161 gtgttgtaaa tcaacttaaa gttaaagcag ctgaaaaaga tatatcactt ttatttgaat
     2221 ttgaaaatga atttatagag aacattcaag gagataaaaa tagattaaga caggttctca
     2281 taaatttaat acaaaattca tttaaattta cgagtcaagg tggttacata aaagttgtag
     2341 cttctcaaga tgaagagata acaaccatat cagtagagga taatggttca ggtatagaaa
     2401 agcaaaattt aaataaagtg ttagataaat ttttccaaga agattataat aaggctggta
     2461 gtggattagg actagcaatt agtaatgaaa ttgtaaaact tcatggtgga agaatgaaaa
     2521 tagaaagtga aaagaatgtt ggaacaaaaa taacttttaa tataaaaaat aaatttgcta
     2581 aacaagcata aattcttaga aagaggatta agatgagaaa cataaaaatg atagtagcat
     2641 acgatggttc aagatataaa ggatatcaaa aattaggtga caataatatg actattcaag
     2701 aaaaacttga aaatgtttta agtaaaatga ctaatgaaac agtggagata ataggttctg
     2761 gaagaactga tatgggagct catgcaagag gacaagtggt aaattttaga actaattgta
     2821 tggattcttt ggataaaatt caaaaatatt tatatgaata tttaccagaa gatatagttg
     2881 taaaaactgt tgaagaagtt gatgaaagat ttcattcaag atacaatgta aaatcaaaga
     2941 cttatatgta taaaattgac aataataagt atcatagtcc ttttataaga aaatatgcta
     3001 ctcatgtaag taaaaaatta gatttagata gaatgaga
//
LOCUS       DAGSHT010000290         2990 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.296, whole genome shotgun sequence.
ACCESSION   DAGSHT010000290 DAGSHT010000000
VERSION     DAGSHT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.296"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            273..1022
                     /locus_tag="MJB72_003506"
     CDS             273..1022
                     /locus_tag="MJB72_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264565.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            1035..>2990
                     /locus_tag="MJB72_003507"
     CDS             1035..>2990
                     /locus_tag="MJB72_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264566.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGSSFSQYIPVIIAIFL"
BASE COUNT         1089 a          402 c          430 g         1069 t
ORIGIN      
        1 tacaagcaat aaagccataa atattgaaaa tttcaatatt tatggcttta ttttattttt
       61 tttaatttaa atccacatta tgccattata tatacctatg cactctttta aattaaaatc
      121 ttgtccatca tcccaaacct aataaagtta gattttaaac aatatattgt gatataagta
      181 atttgtgtag aattattgcg tgatttactc atgaagaaac aataaggtta atatataatt
      241 tgactatatt aagacgaaag gagacatata caatgaatac aatattaaaa gtaaataaca
      301 taactaaaac ttataataaa gggcaacagc cgagtttaga taaggtatcc tttaatgtat
      361 cagaaggtga atttataggt ataatgggag cttctggctc tgggaaaaca actctactta
      421 atgtattatc tacaattgat aaaccaacaa gtggagatat tttcataaat aatataaatt
      481 tgaaatcgct taatgacact tgtgctgctg attttcgtaa ggataattta ggctttatct
      541 ttcaagagta ttttttacta gatagtctta caattttcga gaatatttca gtacctctta
      601 cacttcagaa actcaatcca gataaaatag aaaacatggt aaagagtcta gctgaacgat
      661 ttggcatagc ttctcaatta aataaatacc caaatgaatt atctggtggg caaagacaga
      721 gagcttcagc agttagagca atagtaaaac atcctagtat tctctttgca gatgaaccaa
      781 caggagcttt ggattctaat tcagctactg aactactcca ctcactacag gaagcaaatg
      841 aggagttgaa aacaacaatt ttaatggtaa cacatgatgc ctatgtagca agttttgcaa
      901 atagaatttt gatttttaaa gatggtcata tcattaaaga attaaataaa caacagaata
      961 atagacatac attttatgag ttgattattc aagaaattgc aaaattggat actaaaagat
     1021 aggaggataa tttaatgaac attttttata cggcaataaa gaatcttaaa aaaaactttt
     1081 cattctattc tctatatcta ctttcagtct cttttgttat tactgtattt ttcgcattta
     1141 cttcattttc tgttaataag ataatgctag aaaaaatttc aactgatggt agagttgaat
     1201 ctatgtgtaa tgttatttcg atatttttga tggcatttgt catcttctat atggcatact
     1261 caaatcgatt cttcttgaga cgtcgtacaa aagaattagg tatatatgct ttacttggct
     1321 atcgtaagtt tacaatatta tcgctactta cctttgaaaa tgtattaatt tgtagtggag
     1381 cttttattat tggattggta ttaggagcat tggcacataa gggtattgtc tttggaatta
     1441 cagcactact taatttatca attgataatt cacagatacc attttttaat ataaatgcaa
     1501 taaataaaac aattatcttt atagcattag ttgttttagt cttagctgta tcaaatggta
     1561 aattttttta taagactcca cttgtaaaat tgataagata tgaaaaaagt gcagaaaaaa
     1621 aattaaagtt cagaaaaatt ccagccttta tggggtttat tatgatttta agcgggtatt
     1681 gtttatcact agatattctt cgtggagcta aatcagtatg ggtaagtatt ggattttatc
     1741 aaatgggctt gataactatg attatcattg taattggtac agttctcttt atcaattcct
     1801 ttcttccata tattatgcaa aatagtaaaa aaaataaacg tactttctac acagatatga
     1861 agataatagc tattccaaac tttatctatc gtatacgttc aaactctaaa actctaatta
     1921 tgcttacatt actctctgct actactttga cagtttcaag tgttatggca ctgtccttat
     1981 actaccctat tgcatctgtt tcgcgtattg caccttcaga gatagaattt agagtagaaa
     2041 aaaataaaca aatagaagat gtaaaacaaa ttgtcagtca atatacgtct aatacagatg
     2101 atattatttt tacacagaca aatatttata aggtaaaatc ttcatcaaaa gaccttccat
     2161 ttgaatatga aattggtaca tctaagaata atttatccaa tagtagttct tctaaagaag
     2221 ttatttcaag agaatcagga tttgaatgta tatctttttc tgattattat actttattaa
     2281 aggcacaaaa tcgaacaaag ctactaaaac aacttacccc tttaagggat aatgaatgta
     2341 ttttaatgaa atatcaacca aataatgatg gatatagtga aaatggaacg atttatccat
     2401 tacttgttaa tggtaaagaa acacctgtag ctgtaaggga aacatcatta aacaatgcta
     2461 tttcttttgc caatagcgtt ggaacactta tcgttaatga tagtatatat gagcaaatag
     2521 cacaaaataa attaccaatg acaagaattt taagtattaa tggtaaatca attaaaaata
     2581 atgaaaattt atatagtaat atttcagatt atttagatgg aagcccttat ttacaaggat
     2641 attctcacag gataaatgat ttattatcaa ttaatagttc tacatttctg ttaatcggat
     2701 ttcttgtggt attattcttt attgctgttg gaagtatttt atactttaac aatatatcag
     2761 caatttcaga ttcaacatct gattataaaa ttttacagaa aatgggttat actaatatac
     2821 aaattaaaaa aattattaaa aaacaagtat tgccattttt taatattccg tttttacttg
     2881 gtatattgga ctgtatattt gctaccttgg tttataagac tgctttgatg cagaatttac
     2941 ttggaagttc attttcacaa tatatacctg tgattattgc gattttctta
//
LOCUS       DAGSHT010000291         2980 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.297, whole genome shotgun sequence.
ACCESSION   DAGSHT010000291 DAGSHT010000000
VERSION     DAGSHT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.297"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            387..875
                     /locus_tag="MJB72_003508"
     CDS             387..875
                     /locus_tag="MJB72_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY8264567.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            1264..1434
                     /locus_tag="MJB72_003509"
     CDS             1264..1434
                     /locus_tag="MJB72_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264568.1"
                     /translation="MHVYQPKTSEYLLNTSAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(1911..2099)
                     /locus_tag="MJB72_003510"
     CDS             complement(1911..2099)
                     /locus_tag="MJB72_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8264569.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            2261..2611
                     /locus_tag="MJB72_003511"
     CDS             2261..2611
                     /locus_tag="MJB72_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBY8264570.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2613..2813
                     /locus_tag="MJB72_003512"
     CDS             2613..2813
                     /locus_tag="MJB72_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264571.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         1085 a          338 c          381 g         1176 t
ORIGIN      
        1 taattatttt gattttatag atacacggtt agatgatttt atactagaac ataaaaaaat
       61 gtagtataat cttgtttata tcaagttatg gtattggtaa gtaagtataa ttaaatagac
      121 attattttct gggccctctt tttattttca tctgctttta aataatatta ttggcgttga
      181 tatgactgta gttttcttta ttttaatcca ttgcttatat cttttctaac aagaaatgtc
      241 attttaaatc atttccataa attgtaaaat aatagtatat ttgtcataga aagtgatttt
      301 tttgtctata tatggtaaaa tatagaaagt agaaatcaac aaaataaaaa tttatatatt
      361 attaatttta tatggagggg aaataaatga aaaacaaaaa aggatttact ctagtggaat
      421 tattagtagt aattgctata ataggaatat tggcaatagt agcacttcca gcattattta
      481 aaaatataga aaaagcaaag atagctaaac ttgaagctga tataagtgca ataaaaagtg
      541 cgtctcttag ctactatgca gatgaatcaa aatatactga aggaaacata atatggtgga
      601 ctaaaaaaga tggaaaaata acagtaaact ctggtattgg tgatgaagac cctttggcac
      661 ataaaataga aaatttaggc atgccttata atggttcgta cactttagtg tcatctaatg
      721 gtagtgaaga atacttagaa ttaaacataa ttatagatgg agaaataagt aaaagtggtc
      781 tagataaatt agaagaagat tatggtagtt caataaaaat accaaatgat aaaaatatga
      841 taataacttt tttatctaat aaatcagaca attaaaaata aatatatagg tctgataatt
      901 attgttaaag taaaaagagt tggttataaa aatgaaaaaa gcactcctca agaatagaat
      961 gcttagttat aactacagaa gtgtattggc atacacttct attttttatt atatcacata
     1021 atttaaaaat acctattcaa gttgttttta aattatgttg agtaatattc ttgatagttt
     1081 atacattaat gaatttcaaa aaaataagta ctctttatag taaaaagtac tttagtgtat
     1141 gttaatgtcc tatctaataa aattgtaaca tgccttatgc tttagtatga taattttcat
     1201 tttctttatt gttgcaactt atactatagt tttgctaatt tatctgcaaa cacaacttct
     1261 ttattgcatg tgtatcaacc aaaaacatca gaatacttac ttaatacatc tgcaaaatcg
     1321 tatctttcat ctatagtatt cttatttgac tatataacaa aaaatgaatc gagatatgcc
     1381 ttttttataa ttgattttcc atttttatca ttagatatat tttataaatt ttagcatatc
     1441 atttgttatt ttctgatatg ctattgatgt tttaaatctt ctatttgttt tattattttt
     1501 tacttttcca attatacatt tataggagtt atatttatgt gatttatgaa aaaatgctat
     1561 gcatttaagt tctaattttt tgataataca tctaactttc ttttgattaa taatgatacc
     1621 actgtttttt aatgtggctt ccctgaggca cacatctttt ttaaaaactc aagctcatct
     1681 tttaaaaatt tattttcata ttctaattct tgttctcttg ttaatttatt ttttctttag
     1741 tgtttttgtt tttattcatt atggtcttcc tttcattttg gaaaaggcat caacaccact
     1801 atgtaaaaat ttttattttc atgttgctat aactgtgcta ttgtttattc caaaatactt
     1861 agcgatttat tgtacactat atttagttat catcatatag tttataacat ctaacttgaa
     1921 ccatatagta tagaaaattt ttttattttt agttcactct ctcctaatat attaacccat
     1981 ctttcaactt gagaatggtc ataaactcca tattgtttta ctattaaaat atctcttcct
     2041 tctctattca aataaaattt agctactttt aatttgaaat taaaatcata tttagtcata
     2101 cataaacatc tcaagcagta gatttttgct ctaacaatta tggtgcagta caaatattgt
     2161 ataatactga ttttctattg acaaaagaat ataaaagaga tatatttgta tcataaagat
     2221 ataaatatat ctcttttaaa attactaagg aggataaaaa atgattcaga aaaaattttt
     2281 tatgggtttt tgtggtttcc ttggattttt aagtcttaga tatttttcat ctggcaatgt
     2341 tgctgacctt acatatattg gattttttgc ttttttctca aactttatta tagcaaaaat
     2401 aaatggtgat aaagcagatg aaaggtatgt acaagatgaa aaagcagcta tggcatttac
     2461 aggacagctt gctattatag aattattcat tttatggtgt attactatag tgtctaggaa
     2521 tatagaatta atgtgtgttt tactatcaat tacatatgca atcactttaa atgtttatgc
     2581 tataaaactt tatattttag aggaaaaata atatgcctaa attcacttgt aacctaaaaa
     2641 aatatagaca attaaaagaa ttaactcaag aacaattagc agaaaaagta ggagttagaa
     2701 gagaaaccat tatgcgttta gaagcaggaa agtataatcc ttctttgaaa ttagcaatag
     2761 atatatcaaa aatagtagat acacctattg atgaattgtt tatttttgat tagtttttgc
     2821 attattaaaa atgtgtttta aatacaaact taaattcaat ttttaagtca ctcctgacag
     2881 aggggctttt tattttttca gataatttaa cgttcatata taaatttatt aatttttcag
     2941 ctagatatgt tgaataataa ctaattctat tgtattatta
//
LOCUS       DAGSHT010000292         2963 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.298, whole genome shotgun sequence.
ACCESSION   DAGSHT010000292 DAGSHT010000000
VERSION     DAGSHT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.298"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            70..1179
                     /locus_tag="MJB72_003513"
     CDS             70..1179
                     /locus_tag="MJB72_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8264572.1"
                     /translation="MDFKSLGISENTINILKKLGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDHLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRSLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1556..2740)
                     /locus_tag="MJB72_003514"
     CDS             complement(1556..2740)
                     /locus_tag="MJB72_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264573.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1122 a          446 c          407 g          988 t
ORIGIN      
        1 atacttacaa atatgatatt attaattggt taataaaaaa gaaaattttt catgaaaggt
       61 aagataacaa tggatttcaa aagcttaggg attagtgaaa atacaataaa tattctaaaa
      121 aaattaggaa taacaacacc tacacctata caaaaagaaa gtattaaact aataaaagaa
      181 ggaaaagatg taatagcaga agctcaaact ggaacaggaa aaacactagc ttttttactt
      241 ccgatatttg aaaatatatc attagatata aatgatatac aagtacttat actttctcct
      301 acaagagaac tagctattca aataacagaa gaagctatga aacttaaaga atctaaagat
      361 gtaagtattc ttgcagcata tggaggaaaa gatattggtt ctcaaataaa gaaactaaaa
      421 ggaaatattc atatgattat agctacacca ggtaggcttt tagaccatct aaatagaaaa
      481 acaatagacc ttagtaagtt gaagactttt gttttagatg aagcagacca aatgttgctt
      541 atgggattta aaaatgaagt ggaagctatt ttaaaagaga cttcaaacaa aaagcaaaca
      601 ctatgctttt ctgcaactat aaattctcaa gtaaaaaaat tagcttatag atacacaaaa
      661 aatcctgttg tagtttctat tcaaaaagaa gaaatcactc ttaataatat aaaacaagaa
      721 gtagtagaaa ctacagatag aaaaaaatta gatgccttat gtaaggtact agatgaagat
      781 aatcctttta tggcaataat attttgtaga actaagagaa gggttgacca tcttgaagaa
      841 gctcttgcta taagaggata taattgtcaa aaacttcata gtgatattgc tcaatcaaaa
      901 cgagaaagaa taatgaaatc atttagaagt ttagatatac aatacttaat agctacagat
      961 gtagcttctc gtggtcttga tatatctgga gtaagtcata tatataatta cgatttacca
     1021 gaaacaccag aagactacat ccaccgtatt ggcagaactg gtagagctgg tgaagaagga
     1081 tatacatgtg cttttattga ccctaaaaat gaacgaatgt taagtgaaat agagacggct
     1141 atagaatcta aaattagtag aagaattata gaattataat ttatagattg cttaaaatac
     1201 caatgccaca caaacactgt aatatataga aagctacaga aaacatgtat caatctatac
     1261 tttgctgtta tactatttta tattaaagaa aaaagaggct atccaaagta ggattagtct
     1321 cttttattgt caaaataatg aaaatatact tgacttcata taaaaaagag tgccttataa
     1381 actaaaagtc cattttgaga caccctcttt ttatctttta ttcttagttt tatattgctt
     1441 gtttatagtt gtttaatatt agattttgca aataatctta ataaacatgc agatatatta
     1501 tctatagtat tcttatctat agtattctct ttagcaatat tttttgctac tacatctaat
     1561 ttacattttc atttaattcc gctggccatt tagcttctgg attttccttt gcaaacttct
     1621 tattcttata atgtcctagt gtaaggaatg gaattactat agtgaataga ccaagataac
     1681 cacaatatcc atatccatat ttaacaatat tacttaatcc tgtaagtgat ataagcattg
     1741 acactaccat tattgcacaa gctgtaaata cccttctttt cattcttgaa gaaaggaaag
     1801 aaagtgcttt atggtcttca aatctattta caagaccaaa tatagtagtt actcctgtag
     1861 acattaaaca aaggaataat gcaacattat aaattatgta tatagtatga tttccagatt
     1921 gacctgcaac ataaagagta gggagtgttg tttgcccagc agcaataatt tctctatacc
     1981 atcccattag catagttaca gcaagtccta atgcaattgc attcataaca aatgaaatta
     2041 tcattgcctt agtagcttct tttgagttct taagaagtgg tcttgaacat cctgcaagtg
     2101 taggtataac aaccgattga aaacctgcat aaataaaagt attaagtatt ggcttgatat
     2161 agccaggtgt gaaactcgtt ctatttgata atatttcagt tatttctggt actttagctt
     2221 ttataccaac aataaaaata gtaagagtac atactagaat agctattgtc attgctgtag
     2281 ctgctttaga tacaagcata gcaccaaata tagtaaagac aagaagtaca actccaataa
     2341 taaatacagc tacaaaataa tttactccta tactttgaaa tactgctgct gcacctgcta
     2401 taacagcact tacagctatt aaaaccatta gatagtaata tatctcccat attatttcaa
     2461 gttttggata tggtctccaa agctcacaaa ataaatcctt ataattccta caattattat
     2521 tattatacat tatgataact tctctcatgg tagcagtaag tataatcatt gctaaaattg
     2581 ccataagtgg tgctgtccat ccatactgca catagtactg agtttcttga ttccctgtag
     2641 caaatcctcc tcctgcatgt gaactaaaaa gaacgcttgc aacaccaaaa atggctaaca
     2701 tagagcccgt tacttttgct ttctttacac ttttttgcat gaaatcatcc tccctaaaat
     2761 gatattaaaa gtttttattt taacacttta ttgataaaac aataacattt ttgctcatta
     2821 aattcaacta tatttttcca atgactttac attttcaact tatttctacc tttatattaa
     2881 atatttttta tatatttatt ttatttttta aatagtattc aaattcactt tgttttttaa
     2941 ttttatgaaa tatctttttt aat
//
LOCUS       DAGSHT010000293         2916 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.299, whole genome shotgun sequence.
ACCESSION   DAGSHT010000293 DAGSHT010000000
VERSION     DAGSHT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.299"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1775)
                     /locus_tag="MJB72_003515"
     CDS             complement(<1..1775)
                     /locus_tag="MJB72_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8264574.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSL"
     gene            complement(1971..2663)
                     /locus_tag="MJB72_003516"
     CDS             complement(1971..2663)
                     /locus_tag="MJB72_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264575.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
BASE COUNT          971 a          477 c          249 g         1219 t
ORIGIN      
        1 tctaaggaat attttgctat attagataaa agattttcaa atactctata cattcttctt
       61 ccatctgccc atatatacac cttatcattt ggaacattta attttaaata taaattattt
      121 ttagataatt tttcttcaag ttcacctata ctttgtctta atagttgcac taaatcaatc
      181 ttgctaattt gtaattctat attgccacta cttactctac tcgcttcaaa taaatcttct
      241 atcaataatt ttagtctttt tgactttgac tcaagtacat ttatataatc atttatatac
      301 tctggttgta ttgattcttc ttttttaatt aaatctacat aatttattat agaagtaagt
      361 ggagttttca aatcatgaga tacatttgtt ataaggtcag acttcattct ttcacttttt
      421 acttgctctt ctatagctct atcaagacct tctccaatat tatttatatt ttcagctaaa
      481 gacgtaaaat tatcattccc agtaattttt atcttataat ctaattctcc cttttttatt
      541 ctttctgtac cttctattac gtaactaaga tatgaaagtc ttttagttat atatattgta
      601 aatcctaaaa aaatacatat agaaaacagt aatcccatac ctatacttcg agtgctaatt
      661 accatgatta aagctaaaga tattatcact ataacaataa ctttccttgc taaagatact
      721 ttctttatag cgttactgac ctcccttaca gtttttatta accataaaat cactcttact
      781 ataatacttg tcttaaatat acacatttta ttttctaaat aactcaattg cacatttata
      841 aaaaatatat tatttgatac tatcagtaaa gataaagaaa ctaatgcaat tacatcaata
      901 tgataagata cagcaagagc atatatagtt aaaattgcaa ttgaaataga tactaatatt
      961 acattaaatt ccataggtat cttactgtat attagtaaaa atacattatt tctttttaaa
     1021 acttcacctt ttattttgat atataaaatt atagataata aacatataat tgctaaactt
     1081 aaactagcta caaaaaataa tcttactttt tctgtagctt gtttaaatat ttgaaaatta
     1141 gtatatacta ttccatcttc tatagttggc tcttctggaa aagatacata tgcctcaaaa
     1201 ccttcatcta cttcatttag ttccagactc agattattca gttcaaattt ttctccattt
     1261 attgttttaa tatatgagct ctttcctatc ttatttatta tctctacatt acaatagctc
     1321 ttagtttttt gtttaaaatc atataaacta gcatatgacg tatttgtata aaatactcct
     1381 gtgtatgtat taataacata cacttttata ttttttagat aacttaaata ttcttcagaa
     1441 gcttgttcat taatcatgat tttatcatac tcagaatcac tactacttaa atctatactt
     1501 ttaccttctt gttggaattt acctttttct ttactagtta atttaccacc tacagatagc
     1561 ttaaacttgg gtaatagact tgccattgtt ccttcgtcaa atatattact atcataaaaa
     1621 tcatcagata tatcaaaaat cccactcttt gtagaagttt tttgtatatt gtatttaggc
     1681 tcagcaagtc ctattgtcac caacaatata gttaatggaa atgagataga tattatgatt
     1741 aatgaaacta actttaatac tctatatttc tccaagttgc ccacctcatc tacattgtca
     1801 gtattttata taaaataaag tttaagaaaa gcataatccc catagagtaa attaaactct
     1861 cagagaaaaa attaaaacat attaaaattc ctaacatttg tgtaaatgta aaaatgacta
     1921 aaatcaaaat gcaattaaat attaaactct tttcttttat aatttttcca ctaaaatttt
     1981 tcaatcttat aaccaactcc ccacaccacc tttaaaaatc ttggttcttt tggatttatt
     2041 tcaatctttt ctcttattcg tctgatatgt actgctactg tattttctga attaaaactt
     2101 tcctcattcc atactttttc gtatatctca tctattgaga aaactcgtcc tttattttct
     2161 aaaagaagtt caattatttt atactcaatt ggagtcattt ttatgttttc accatcaatt
     2221 ttaacttctt ttgttagtgt atttaactcc aaagctccac tttttaaaat ttctttacat
     2281 gtatcttcat aattacctaa agttacatat cgtcttaagt ttgatttaac cctagctata
     2341 agctctaata gattaaatgg ctttgttata taatcatcag ctccaatatt taatcctatt
     2401 attttatctg tatcttcact ttttgctgaa attaaaatta ttggaatatt tctttcttct
     2461 cgtattttta cagttgcttt gattccatct aatttaggca tcattatatc cattagtatt
     2521 agatgaatgt cattctctgt tattatttcc aaagctttca ttccatcata tgctttatat
     2581 attttaaatc cttctgattt aagatatatt tctatcgaat caacaatttc tttatcatcg
     2641 tctactacta atatattgta catattttca cttcctctat tgtataataa tacttatcta
     2701 tattatacaa tatctttcac tccctttctc aacaatgact aaattgttat attactttgg
     2761 cttacatttt tgtaactttc ccttataact atataataaa atataaatct taagaaaata
     2821 ttatcctaat tcttaaatat ttcttaagat ttaagataaa ttgaggttta tttgaataaa
     2881 ttgatttgaa ttatgttgtt ttcagattac tttcat
//
LOCUS       DAGSHT010000294         2897 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.300, whole genome shotgun sequence.
ACCESSION   DAGSHT010000294 DAGSHT010000000
VERSION     DAGSHT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.300"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(440..1456)
                     /locus_tag="MJB72_003517"
     CDS             complement(440..1456)
                     /locus_tag="MJB72_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY8264576.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKESGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWN"
     gene            complement(1785..1967)
                     /locus_tag="MJB72_003518"
     CDS             complement(1785..1967)
                     /locus_tag="MJB72_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264577.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            2280..2699
                     /locus_tag="MJB72_003519"
     CDS             2280..2699
                     /locus_tag="MJB72_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBY8264578.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
BASE COUNT         1103 a          412 c          342 g         1040 t
ORIGIN      
        1 ttacttctaa atttataata tgtttaattc ataaatataa ttggataaat aatatcaaat
       61 gttctattct atcatccatg aatgaaaggt atacctatat cagtaatttt agcaattagt
      121 gaatgagata tatcaattct gcaaatactt ttaaggtaac ttgaaatatt taaggttatt
      181 ttttcaaact ctgcttctaa caaattaatg aattgtactt atgaataaat attttaacat
      241 atattgtata tttgtatcta ttttaatatt atattattaa ataaatttag ctaatatttt
      301 gtttttgtta aatataaaaa gtgtctacca taatttgtat agtaatattt gagattttaa
      361 ttatcactat tttttgtaag aatacacaat ttatatttaa atacctgaca gaatatttaa
      421 atataaattt gttttaaaac taattccata taactctacc atctatatcc ttaacatttt
      481 gacaaccagt caaaatcata gtctcacata actcattcct aaccttctct ataaaaaact
      541 ccacaccatc aagaccaccg ccaaaagaag cagtaacaaa aggtcttccc atcaaaacag
      601 catcagcacc caatccaagc atcttaacta catcaacacc agtcctaact ccaccatcaa
      661 caagtattgt tatctttccc tttacagact tagctatatc tggaagtacc tcacaagtac
      721 caggagtata atcaagtact cttccaccat gatttgaaac aataatggca ctagcaccag
      781 actctacagc catcaaagca tcatctacag tcataattcc ttttataata aaaggtaatc
      841 tagtggattc aaccaattct ctaatctcat caattgtttt aggagaaaaa ggattttctt
      901 gaaattgatt attaataagt ccacaagcat caaggtctac accaactgca aaagcacctg
      961 cttcttcaga caatctaatt ttatctatta tctttgaatt attccaaggc tttataaata
     1021 caattccatt tcccctatta tccttcaata catctaaatt atccagtaaa aaagtatcta
     1081 cattagtatc gccaaccata gcataaattc cagaattaga acaacctcta acaacaggct
     1141 ctatgtattc tttttcggaa actttaccac ccatgttcaa tatagtacct gatacaggtg
     1201 cagcgaatat aggagaactc atttttctac caaataattc tatggatgta tctggctttg
     1261 aaacattatg tatgactctc atatttatct ttattttttc tagacttttt ctattttcaa
     1321 tgaaagacga accactttct ttaccaccca taccaggaac atctccagca caagctaatc
     1381 cattacaaat tttacatacc ttacatgttc cattaaagtt ctctctagct gattttagta
     1441 agtcattgta attcatacaa aatctctcct ttttataata ttaaaattga gtattactat
     1501 attgaaaata tcttaattgt aaatataggg atgttattat gatgagctaa actagataat
     1561 acatattaga tattttttat tgttgtagat agttaaatta tatcatatta aatagtcaaa
     1621 ttttgaaaaa aactaactta tttaagtata cattttgaaa aaattaaaaa agctatatat
     1681 agatttttaa agaaatctat atatagcttt taagtttatg tcaatctttt ttgttaatga
     1741 ataaataata tagaatacaa tatacaaaaa agtttcttga aatgctaaga taatccccat
     1801 ttacaaagca acatcattat actgtgtaaa tttgaatcaa caatgttttc accatatttc
     1861 atcataaaat cctctaattg tgtgtcaata aatgaataat atccacttat actatcgatg
     1921 tatgcagtca ttaagtgtat acattcatca cttttatctt tatacatata tatctccccc
     1981 tatatacaat atataaattt agataccaaa tcgtatcata ttaaatagtt aagtgtgtaa
     2041 catctatgca gttatatatt tatatcatta tattaataac taaagtgagt tttgacaata
     2101 gattttggga aactggatgt tcaaagtgca aacttggatt ttaaaatcat taattaagat
     2161 agcgcatagg aatatatgta tacataactt aattataaag attataaaca attactttta
     2221 ttaactttat ttaaatggta gaataacttg gagattaata attataagga ggatataata
     2281 tgagtttata taagtttata tatgatgata aggaatattt attaaaagag gagaactgta
     2341 gtgctttaat aaatgatgaa gaaaatccag ttcaaggagt aagtatttca aaaataattg
     2401 atatacttaa tgaagcagaa gaagttgatt ttgatgtaga atattatcaa gaagcatgtc
     2461 cattatgttt agaaggagta aaagaaaaga aaaaattctt tcctttttta gaatatcact
     2521 tttatatttt cagtaaagat ggagaatatg taataagcaa tatatctgtt gattataaag
     2581 gactatcttt taataaacta tcaagggcaa ataaagttga taatagttat attgtaagtg
     2641 tgataatttg cgaaaattgt caggattata tagttcagat agaaaattgt atagtttagt
     2701 gtaaagtatg tcttaattta agagaaattt ctaaattgag acatcaactt ttttcaaaaa
     2761 tagtatggaa aattactaaa tttatgagat aatgtaagtc ggaggtgagt tacaatatat
     2821 agtaaatatt tggaagagat agatgataca attttagatt caatgtttaa actaaccttg
     2881 aatgaaagat tattgac
//
LOCUS       DAGSHT010000295         2850 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.301, whole genome shotgun sequence.
ACCESSION   DAGSHT010000295 DAGSHT010000000
VERSION     DAGSHT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.301"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            282..785
                     /locus_tag="MJB72_003520"
     CDS             282..785
                     /locus_tag="MJB72_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBY8264579.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            832..1791
                     /gene="moaA"
                     /locus_tag="MJB72_003521"
     CDS             832..1791
                     /gene="moaA"
                     /locus_tag="MJB72_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBY8264580.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1795..2265
                     /gene="moaC"
                     /locus_tag="MJB72_003522"
     CDS             1795..2265
                     /gene="moaC"
                     /locus_tag="MJB72_003522"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBY8264581.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2301..2732
                     /locus_tag="MJB72_003523"
     CDS             2301..2732
                     /locus_tag="MJB72_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBY8264582.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1191 a          251 c          543 g          865 t
ORIGIN      
        1 aaaatatgga caatgttata ataattatag aaattaaaag tacttacatt cattatgtat
       61 gtgacatgta taattagttt tatatttaat acagttttga agtaatatct tcattttgag
      121 aacgttggaa taataaaaaa tgtattatta taggtgatat aaataaaaaa taaatataat
      181 atgttatatt gctgatagaa aagagaataa aagaaaatag gttttattaa aagagatttt
      241 tatttatagg acttaatcaa tgtatagagg ggtgagaaca tatgtttaat gttgcaatta
      301 taacacttag tgataaagga tatgaaggaa aaagagaaga tataacagga aaaaagttaa
      361 ctgaatttgt tgaaaataca ggagtttata aagttactga atatgtactt ataaaagatg
      421 ataaggaaat gctaaaggaa aatattataa gactttgtaa tagtaataaa atagatttaa
      481 ttcttacaaa tggaggtact ggtttttcaa aaagagatat aaccccagaa gcaactaagg
      541 aagtgttgga aaaagaaata ccaggtctga gcgaatatat gagaatgaaa tccacagaaa
      601 ttactaaaaa ggccatacta agtaggggtg tgagtggaat aagaaataat tctataataa
      661 taaatttacc tggaagtcct aaaggtgcag ttgaaaatct aagttttata attgatgttt
      721 tagaccatgg catagaagtt ttaagaggag aagctacaga atgtgccact aaaaaaagtg
      781 aatagtatat aatctagata gataaaaaat agacaaagag gtagataaag tatgattgat
      841 aagtatggaa gaaaaataga ctaccttcgt atttcactta cagataagtg taatttaagg
      901 tgtgtctatt gtatgccacc agatgtaaaa tttgataaaa attatataaa tgaaaatcta
      961 agctttgaag actacaagtt tattataaag gctatggctg aacaaggtat aacaaaaatt
     1021 agatttactg gaggagaacc tcttttatat cctaaattaa atgagcttat aaaatttaca
     1081 aaagaagaat gtggaataaa taacatagga atgaccacta atgctattgg tctttctgat
     1141 agaatttttg agcttgaaaa atgtggctta aataaagtta atataagttt agattctttg
     1201 aaggaatata agtataaatc cataacaaga ggaggaaact taaaagatgt cttaaaatcg
     1261 attgaatctt gtttaaattt aggtataaaa gtaaagttaa attgcgttgc tataagtggg
     1321 tttaatgatg atgagcttaa agattttatg tttttaacag ttaaatcacc aattgatgta
     1381 agatttatag agcttatgcc attaggtgag gctaatagag tttataagaa gggttatttt
     1441 aatgtaaaag aaatgattga aaaaatatat ggtttatata aagtcaaaaa tgatgaaaaa
     1501 agtacagcag aatattatat gtttaaagga gctaaaggaa gaataggaat tataactcca
     1561 ttaagctgtt cattttgtaa cacttgtaat cgtattagac ttacttctag tggtgcaata
     1621 aaattatgtc tacattcaaa agaagaaata gatataaaac catttttaca taaaccattg
     1681 ttatttagag aatctatgaa agatataata aaacaaaaac cagaaaaaca tcatttaata
     1741 gaaaataaat gtagtgatac aaataggcgt atgtatgaaa ttggaggata agtaatgtta
     1801 acacatataa atgaaaaagg gtatgccaag atggtggaca taagtgaaaa agatgatact
     1861 aaaagaacgg ccgtagctac agctacaatt actcttagta aggaagcatt agaaaaagta
     1921 aaaaatggtc agataaaaaa aggagacgta ttgtctgtag ctcaagttgc aggtataatg
     1981 ggagctaaaa atacatcttc aaacatacca atgtgccatc agattaaatt agttggatgt
     2041 gatttagaat ttgatgtaga tgattctatg tctgctatta agatatacgc aacctgtaaa
     2101 tctcttggga aaactggagt agaaatggaa gctttaaatg catgttcttt agcagcactt
     2161 acaatttatg atatgtgtaa agcagttgat aaaaaaatgg ttataagtaa tatccatctt
     2221 gtggagaaga ctggtggaaa atcaggagat tttaagttcg agtaaattaa taatatattg
     2281 ggttaaaatt tggaggaaat atggcgaagg tagtgtcaat aaatataagt gagaaaaagg
     2341 gtgtaattaa agttcctgta caatgtgcag agctaaaaat aaatcatggg atagttggag
     2401 atgcacatgc aggaaactgg catcgtcaga taagcttact tggaaaagaa agcatagata
     2461 aaatgaaaat gaaaggcttt gaccaattaa aatatggaga ttttgctgaa aatgtaacta
     2521 cagaagggat agaagttttt tccctgcctg ttggtacaaa gttaagaata gggcaatgtg
     2581 aagttgaagt aactcaaata gggaaaaaat gtcacaatgg atgtgaaatt aagaaattga
     2641 caggtgactg tgtgatgcca cgagaaggaa tttttgtaaa ggttctaaaa gaaggaatta
     2701 taaatgtgaa tgattcaata gatgtaatct aatgaagtca ctattaaaat ataattataa
     2761 gtaaatacta ttaagattta aatatatatt ttaagagaga attaatttta ataaaatatt
     2821 ttagttataa attggaatat tggttatata
//
LOCUS       DAGSHT010000296         2738 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.302, whole genome shotgun sequence.
ACCESSION   DAGSHT010000296 DAGSHT010000000
VERSION     DAGSHT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.302"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..567
                     /locus_tag="MJB72_003524"
     CDS             <1..567
                     /locus_tag="MJB72_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8264583.1"
                     /translation="SYWNVLEYKVDVDEKTYIITMANDITKLYEENKRNLHYSITDPL
                     TGAYNRKYLNDRFDIFIGDYIVLIDLDNFKMINDYEGHNVGDKILCDFVSLLNKELIN
                     STSIIRLGGDEFIVIFSSDVDKNYVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRN
                     LKLTIVELDKKMYKNKEKNKKKFDKNDY"
     gene            807..2540
                     /locus_tag="MJB72_003525"
     CDS             807..2540
                     /locus_tag="MJB72_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8264584.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
BASE COUNT         1135 a          307 c          408 g          888 t
ORIGIN      
        1 agctattgga atgttttaga atacaaagta gatgttgatg aaaaaaccta cataattact
       61 atggccaatg atataacaaa actctatgaa gaaaataaga gaaatcttca ctactcaatt
      121 actgacccat taactggagc atataataga aaatatttaa atgatagatt tgacatattt
      181 ataggagatt atattgtact aattgactta gataatttca aaatgataaa tgactatgaa
      241 ggtcataatg taggagacaa gattttatgt gattttgttt cacttttaaa taaagaatta
      301 ataaattcta cttctattat acgattagga ggagatgaat ttattgttat attctctagt
      361 gatgttgaca aaaattatgt ttattcacag ctagaagcct taagagaaaa ttttttaaaa
      421 gtattctcta agtataagta tctttcattt agttatggtg ttgatacagt aaaaagaaat
      481 ttaaaattga caatagttga attggataaa aaaatgtata aaaacaagga gaaaaacaag
      541 aaaaaatttg ataaaaatga ttattaacat tgttatttta aaataaatta aaattatctt
      601 tcttttttta aacttgtaca ttagtttata aatatgtatc tatacaagat attgacatta
      661 agtactgaag catatattgt ttcagttctt ttttgttaaa attaacttat ttaacaaaga
      721 tatcatagtt taacaaagct tttaatttaa atatattgat gtataatata attaaaatat
      781 aatagcaaaa agaaaggagg aatatcatga gcatactcct aaaaaaagca cccaaattag
      841 caaaacatat tataacaagt ttttatataa atcgagatat caatgaagta ttaaaatacc
      901 tttgtgaaaa tgttacatgg attggaccag gagaacagga atttctcact tcttttaatg
      961 aaataaaaaa ttatttttat gcaggtcaag atgaaattcc ttcttgtgat ataaataatg
     1021 atatatttga aactgtaagt gaagatgaga atagatgtat ggtattagga agatatactg
     1081 ttcgaactaa agaaaatgct caaatgattt tagaggtaaa tcaacgttgt acatttgaga
     1141 taattgagga tagaggaaaa ttattaataa agcacatgca catatcaaac ccttatggtg
     1201 aaatgcaact tgatgaatac tttcccacta aaattggaac tcaaagttat gattatttgc
     1261 aaagattact aaaagaaaaa acagaagtta ttgaaatgat tacaaataat atcaatggtg
     1321 gcttaaaagg tagcaatgat gatagtacat actcattttt ctatgtcaat gagggattgc
     1381 ccaaaatatt aggatacaca tatgatgagt tcatggaaat gagtggtggt tcagcagtgg
     1441 gtgctgtata tccaccagac ttacctaaag cactagaaga ttgtgaacga tgttttgcaa
     1501 aaggacctac atattcatct gaatatagaa tccgaaaaaa agatggaaca ttaatgtggg
     1561 tattagattc tggaatgaaa tcgttaaata gtgatggcat agtaaaaatt aatagtatta
     1621 ttactgacat aacccaacta aaaaacatag aatctgaact aaaattagaa agagagcgat
     1681 atcgtattgc tttgcaaaat attacagata tcatgtttga gtatgatatt gaaaacgata
     1741 cccttataaa atatcaacgt gtagaaattg acaaaaaaat tgaattagaa aattttgaaa
     1801 caaaaaatta ctccaaacta ttagaatctg gtaaaattat tcaccttgat gatattggaa
     1861 aactactaga agtacttcat ggaaatcttc gtgaaacaat tgagataaga caagtaaatt
     1921 cattaactaa aaatgaatgg agatggattc gtgttcaatg ttcagttata tatgatagtg
     1981 accataaccc tatcaaaaca ataggcgttt taaaagacat aactgaagat aaatctaaat
     2041 tagaaagttt aattaatcag gctcaacgtg acccactaac acaattatat aatcaaagag
     2101 tatcacaaaa tcttattgaa gagtatctat gtagttctga ttccaaaaat aatgatgctc
     2161 tattagttat tgatattgat gattttaaaa ctgtaaatga tacttttggg catctagagg
     2221 gaaatgaagt tttagttgct gtatcaaaaa tactattaca taacacacat gataaagaca
     2281 tagttgctcg aattggtgga gatgaattta caatatttat aaaatcatta acaaaggatt
     2341 taataataaa aattactaat gatattttaa atgatgcgag taaaataaaa gtaaaggaca
     2401 atcataaaat aacattaagt attggtattg ccttcactga tgattcaact aaattatata
     2461 aagatttatt ttctaaagct gataaagctc tttatctatc aaaagcagat ggcaaaaatt
     2521 gttatagtac atacgaataa taaagctatt tatacgacca agaatgaacc tttaaattta
     2581 atttcatagt ctaacaatca aagagttgaa gctaatctta tagctagtca actagttcct
     2641 agaaaaaatt taaacaatat atttattgat actgctcatt tttctaaaga gcaaatttat
     2701 ataaaaaatt aaacttttat atacatacag atactaat
//
LOCUS       DAGSHT010000297         2734 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.303, whole genome shotgun sequence.
ACCESSION   DAGSHT010000297 DAGSHT010000000
VERSION     DAGSHT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.303"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            180..857
                     /locus_tag="MJB72_003526"
     CDS             180..857
                     /locus_tag="MJB72_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY8264585.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            1031..>2734
                     /locus_tag="MJB72_003527"
     CDS             1031..>2734
                     /locus_tag="MJB72_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBY8264586.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LK"
BASE COUNT         1097 a          284 c          487 g          866 t
ORIGIN      
        1 aataaataat agaataaatt taaattaaac ataataaaac ataaacaaca aaactgacct
       61 taataaaaca ttgaaatacc aacacttgta aaaaataaca acaataaaca aataaaatag
      121 aataatatta caacttaagt aaataataat tttgaataat aatttacgga ggtattcata
      181 tgtttaaaca cgataaagca ttattaaaag aagtaaaagt agaaagacca aatcctcaat
      241 atgctgtttt aatgcaagaa cagttgggtg gtgcaaatgg agagttaaaa gcagctatgc
      301 aatacttgtc ccaaagtttt agaataaaag accctcaaat aaaagattta tttcttgata
      361 tagcagcaga agagcttagc catatggaaa tggtagcaca aactataaat ttattaaatg
      421 gacatgatgt tgattataat gcagttaata caggcgaaat agaaacacat gtattaactg
      481 gtctatcacc agtcttaata aactcatcag gagctccttg gactgctaat tacgttacag
      541 ttacagggga tttagtagct gacttacttt caaatatagc ttctgaacaa agagctaagg
      601 tagtttatga gtacttgtat agacaaatag atgataaata tgtaaaagaa acaatagatt
      661 tcttacttaa tagagaagaa gcacataatg ctctatttag agatgcttta aataaagtta
      721 aagatacagg ttcaaataga gattttggag ttacagaaga ttctaaatta tatttcgatt
      781 tgtctactcc agggccaaat cacgatacta agatagatat taatcctcct tcttttgaaa
      841 aacctttaaa aaaataattt gatatttttt atgttgtttt gttttagtag ttataaaaat
      901 tatttactag aagaattaat tatatgaaaa attaaatatg tatagttaaa aatgggtata
      961 cacttattat aagtgtatat ctatttgttt attgcaaaac atataataaa ataacaaatg
     1021 aggtggtatt ttggacagta atagaaaacc taataaccta ataaatgaaa aatctccata
     1081 tcttttacag catgcttata atcctataaa ctggtatagt tggaatgatg aggcttttaa
     1141 aaaagcaaaa gaagaggata aaccaatatt tttaagtgta ggatattcaa catgtcattg
     1201 gtgccatgtt atggaaaaag aatcttttga agatgaagaa gttgctgaaa taatgaatag
     1261 aaactttgtg gcaataaaag tagataaaga ggaaagacct gatgtagata gcgtgtatat
     1321 gacagtttgc caagctatga caggtagtgg tggatggcca atgaccataa taatgacacc
     1381 agataaaaaa cctttttttg cgggaacata tttcccaaaa tattcaaggt ataataggcc
     1441 tggagttata gatttattag agaatgtatc tgaaaagtgg aatacaagca gggatatatt
     1501 aataaaatct ggagatgaaa taatagaagc tcttaaggat gattttggtg taaaaaatac
     1561 tgagggtgat ttatcaaaag agatgttgag ttcatcagtt agagtattta aggctattta
     1621 tgatgaaaag tatggtggtt ttggtaatgc tcctaaattt ccaagtcctc agaatcttat
     1681 gtttttaatg aaatattata gtatagaaaa agacaaagat gtgttaaaaa tggtggaaaa
     1741 aactttagat ggaatgtata gaggaggctt gtttgatcat attggatttg ggttttcaag
     1801 atactctact gataaaaagt ggttggctcc tcattttgaa aaaatgctat atgataatgc
     1861 tatgcttact atagcttttt tagatgcgta taaaataaca aagaaagagt tgtataaaga
     1921 aattgctata aaaactatag attatgtagt tagagagatg aaggataaag aaggtgggtt
     1981 ttattcagca caggatgctg atagtgaagg tgaagaaggt aaattttata catttaatcc
     2041 tcttgagata attgaagttt tgggtgaaga agatggaact ttctttaata attattttga
     2101 tataactagt agtggaaatt ttgaaggcaa aagtattcct aatcttataa aaaataaaga
     2161 atatgagaga cataatgaaa agatagctga ccttagtaaa aaggtatttg aatatagaaa
     2221 agaaagaaca tctttgcata aagatgacaa gatacttaca tcatggaatg cattgatgat
     2281 agttgcttta acaaaggcat atagtacttt aaaaaatgac atatatttag agtattcaaa
     2341 taagtgtctt aactttataa ataataatct tgtaaatgag tcaggtagat tattagctag
     2401 atatagagat ggaagttccg actatttagc atatttagat gattatgctt ttttaatctg
     2461 ggcgtatata gagctttatg agtcaacatt taatatgaaa tatttagaaa aggctttaaa
     2521 tttgaatgaa agctgtatca accttttttg ggattatgaa aaaagtggat tttatatata
     2581 tggtaaagat agtgaaaatt taatagcaag accaaaagat ttgtatgatg gagctattcc
     2641 atcaggaaat tctgttcaat tatacaatct tataagactt gcgaaaatta ctggagataa
     2701 tagacttgaa gaaatgtcat ataaacaatt aaaa
//
LOCUS       DAGSHT010000298         2704 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.304, whole genome shotgun sequence.
ACCESSION   DAGSHT010000298 DAGSHT010000000
VERSION     DAGSHT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.304"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..913
                     /locus_tag="MJB72_003528"
     CDS             <1..913
                     /locus_tag="MJB72_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBY8264587.1"
                     /translation="DYKENYSNILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSK
                     NKLYILERLENYNNKEIVDLENLINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKY
                     LHTIGNLTLTGYNSKLSNKTFEEKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTK
                     ILLDRAVEIWKYPSVTYEPPSNSENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTE
                     FYEQVALTLYDLDSVRFNSIVDKTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLN
                     TEAKLNIIRFLLNEFDIELNEVIFSIK"
     gene            complement(1130..1696)
                     /locus_tag="MJB72_003529"
     CDS             complement(1130..1696)
                     /locus_tag="MJB72_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY8264588.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            1862..2383
                     /locus_tag="MJB72_003530"
     CDS             1862..2383
                     /locus_tag="MJB72_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBY8264589.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
BASE COUNT         1171 a          275 c          396 g          862 t
ORIGIN      
        1 tgattataaa gaaaactatt ctaatatatt aagatatatt tttataaata agaaatcatc
       61 tcaaagattt cctactgaca gtgaattttc aaatgctttt gaaactaaag atatatataa
      121 tactaagtca aagaataagt tatatatact tgagaggtta gagaattata ataataaaga
      181 aattgttgat ttagaaaatt taatcaataa taatatgcta aatatagagc atataatgcc
      241 acagacatta acaaataaat ggaaggaatc actaggagat aactataagg agatacatga
      301 taaatattta catacaattg gaaatttaac tttgacagga tataattcaa aactaagtaa
      361 taaaacattt gaagaaaaga aagaaatgga agatggattt aaagatagta gactttatct
      421 aaataaatat atttcatcta tagataagtg gaatgaagaa gaaataaaaa atagaactaa
      481 gatattacta gatagagctg tagaaatttg gaaatatcca agtgttacat atgaacctcc
      541 aagtaacagt gaaaatatat ttacgttaga ggatgatgat gttaatttta caaatgaaag
      601 tatagtatca tttagtatat taggagagtc atataaggct aaaaattgga cagaatttta
      661 tgaacaagtt gctcttactt tatatgattt ggattctgta aggtttaaca gtattgtaga
      721 caaaacttat aaaagagatt ttatagataa tattaagtct agagatgcat ataaattaag
      781 aaaaggtatt aaaatatcag aaaatatata tttagaaaca aatttaaata cagaggctaa
      841 attaaatata attagatttt tacttaatga gtttgatata gagttaaatg aagtaatatt
      901 ttccataaaa taatgaaata tatgcatttt ttgcgtaata gaatattaat tatattaaaa
      961 ataaaatagc acctcttgat atagatttat agctcaaatt tatatcaaga ggttacgttt
     1021 aattaaagta atacaaatac tatctaatta aattattaat aaaaatgtta tataaaaatg
     1081 agtatatagc attttaaaga gtaataaacc ttacataata aatgatttat taatatgaac
     1141 tagaagtatt tttactatat tgttttataa aacctttagg cattttactt ataaaatata
     1201 ccaccataat acaagacaaa ggtttgtcta ctatattact aagaatcctt ggaaagaacg
     1261 cagcagtaaa tatcttctga ccagactgaa ctaaaaaacc aaccaataaa tcagcaccac
     1321 ttccacttaa accaccaaac aataaaactg aaataggagt accaattaaa ggacaaacaa
     1381 tagataaaat tactcctgta ataatagctt ctttataacc aaaacctttt ttcttagaaa
     1441 tatatccaac aataagacca agaacaatat ttacaagggc aaaaggtata tttgtaggac
     1501 ctgatataac acctaaaact aagtttgtac atccaccaat aagagcaccc caaaaaggac
     1561 ctaatacgac agccccaagt acagtaccaa ctgtatctaa aaataataat ggtatattta
     1621 tcatagatat aaccatacct aagacaatat taataactat gcacattgaa gaaaatgtga
     1681 cttttttagt atccaattat aatacctcct aaaaaaatat atacatttat catagtatag
     1741 attataaaag attactaatt gtatatttct atatagaata ttggaaatat agaacaaata
     1801 aataatataa ttatcaaaat atgtagtatc attaaaatta agaataacta aggagattat
     1861 tatgaagaga aatagagaga ttgtagtagt atgtcattgt atattaaatt gtaattctaa
     1921 agtagaggga ttgtcagaat ttaagggagc acaaaatata gcaatagatt taataaataa
     1981 agggtatgga ataattcaac taccatgtcc agaaatgatt atgtatggaa taaataggtg
     2041 gggacatgta aaagaacagt ttgacaattt gttttttaga gaacaatgta ggcaaatgtt
     2101 aatacctcat gtaaggcagt ttgatgaata tttaaaaaat gattatgata taaaaagtat
     2161 cattgcaata gatggcagtc caagttgtgg ttataataag acatgtagtt ctaaacgttg
     2221 gtttggtgag ttaagtggtt gcaatgaatt gatggataaa ataaatgata tacaaatggt
     2281 ggatgggaaa ggtattttta tagaagagct agaaaagcta cttaaagaat ataatttaga
     2341 gatacctata gttggatttg acgagcttag agaagataag tagagatata aatatttatt
     2401 gtctcattat gaaacaccat tatatttggc agaaaaatat gactgatgga cagtacagga
     2461 accattttta agtggagtaa ttagtactta taaagatgta tccactagct gaaagaaaat
     2521 gtagaaaggt aataaatgaa ttaagaagta gataatatat ataaaggagt gtctaaaagt
     2581 gaacttttta gtttatgaga cactcttttt ttatattaaa tcaagtatat taaatcaaat
     2641 atattttaat cattttagca atgaaaaata aatttatcta tattttgaga caacatcttt
     2701 ttta
//
LOCUS       DAGSHT010000299         2704 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.305, whole genome shotgun sequence.
ACCESSION   DAGSHT010000299 DAGSHT010000000
VERSION     DAGSHT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.305"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..619
                     /locus_tag="MJB72_003531"
     CDS             <1..619
                     /locus_tag="MJB72_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBY8264590.1"
                     /translation="ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFI
                     DDLGNQFFIRDKDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLV
                     KKNIENILDNGFTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGN
                     DNSLKGIKNITIENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            594..1421
                     /gene="panB"
                     /locus_tag="MJB72_003532"
     CDS             594..1421
                     /gene="panB"
                     /locus_tag="MJB72_003532"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBY8264591.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1440..2288
                     /locus_tag="MJB72_003533"
     CDS             1440..2288
                     /locus_tag="MJB72_003533"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBY8264592.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1090 a          321 c          423 g          870 t
ORIGIN      
        1 tgcattaatt tattctatcc atcctatatt tgcattttcc aataaaaata ccaacataaa
       61 aaaaatgaaa gacatttgtt tttccataga aggtgataat gaacaagatg atttagttat
      121 tcaaaatttc atagatgatt tgggaaacca attttttata agagataagg atacttcatc
      181 cacttatcat ttagctaatg ttttagtttc aaatttagta ttatctttac tagatatagg
      241 agtaagttac tttattaaat taggtttatc tgaagaggaa tctttaaaag ctatcagtcc
      301 tttagtaaaa aaaaatatag aaaatatact tgataatggc tttactaaag ctcttactgg
      361 tcctgtttct cgtggagata taacaccaat aagaaaacat ctatctgtat tggataaaaa
      421 agatgaggat atttacaaaa tactatcctt aaacctacta aaaataattg ccttaggaaa
      481 tgacaattca ttaaaaggta taaaaaatat tactatagaa aatgcaatcg aaaatttaat
      541 aagtaaatct gaaaaataca aagaaattta caaactttta ggaggaaaaa acgatgaaaa
      601 ataccataca gacttttaag aatgctaaat atgaaggaaa aaaactatct atgcttactg
      661 cttatgacta ctcaattgca aaaataatgg atgagtgtga tataaatggt attttaatag
      721 gagattcatt aggaatggtc ataaaaggtg aagaaaacac tctatctgtt accatagacg
      781 aaattatata tcatacaaaa gctgtaaaaa atggtgtaaa aaatgcctta atcgtaagtg
      841 atatgccttt tctatcttac catgtttcta tagaagatgc agttaaaaac gctggtagac
      901 ttataaagga aggaggagct catgctgtaa agttagaagg tggctcaaac gttataaagc
      961 aaatagaaag cattgtaaat gctcaaatac ctgtaatggg gcatttagga ttaactcctc
     1021 aatcagtaaa ttcatttggt gggtttaagg ttcaaggaaa taccagtgaa acagcaagac
     1081 aacttattga agatgctaaa ttaatagaaa aagcaggtgc attctctatt gtccttgaag
     1141 gagtgcctac gaaaatagct gagatggtaa ctaattctat ttcaattcct acaataggta
     1201 taggggcagg aattaactgt gatggacaaa ttttagttta tcaagatatg ttgggaatgt
     1261 ttggagattt tgttcctaag tttgtaaagc agtatgcaaa tataggagat ataatgaaag
     1321 attcaattaa aaattacatc ctagaagtta atacaggtgc atttccacaa gaaaaacaca
     1381 gcttttcaat aaatgaatct gagttagaaa aattatacta atattaagga tggtacaaaa
     1441 tgcttgtaaa agaaattaaa ttactaagaa atataatcaa agattggaga aaacatggct
     1501 attctatagg ccttgttaca acaatgggat ttttacatga aggacaccaa agtcttataa
     1561 aaaaagctgt aaaagaaaat gataaagtag tagtgagtgt atttgtaaat ccaactcaat
     1621 ttggaccaaa tgaagatttt aatagctatc caagagatat agataaagat tttaaatatt
     1681 gtatggatag tggagcaaca gtagtcttta acccatcacc agaagaaatg tatttaaaag
     1741 gtaattgtac taccattaat gtttctggac ttacagattt tctttgtgga gctaaaagac
     1801 ctgttcattt tggaggtgtc tgcctagttg tttcaaagtt tctaaatata gtaacacctg
     1861 ataaagctta ttttggagag aaggatgccc aacaattagc tgtaattaaa agaatggtca
     1921 aggatttaaa catagataca gaaataatag gctgtcctat aattcgtgaa aatgatggat
     1981 tagcaaaaag ttcgagaaat acatatctct ctgaggaaga acgaaaatct gctctaatat
     2041 taaataagag cttaagttta gcaaaagaag aattagtaaa aggcaatcta aatccagaga
     2101 atataaaaga attaataaca gctaaaataa attctgaaca cttagcaaaa atagactatg
     2161 ttgaaattgt agatagtgag acattacaac ctgttaagca aatagaacat tctatacttg
     2221 tggcaattgc tgtgtttata ggaaaaacca gactaattga taattttaca tttgagttaa
     2281 atatatagtc tataatttta tataatatat ctaaaaaata atagaatacc taaaaagtaa
     2341 taaattacct aaaaaataat aaattgctta gaaaataata gattacaata actaaaattt
     2401 taaaattaaa ttccttaaaa gtatgattaa ttataattat ataagtatat tcttattaca
     2461 cttatattaa tttatattaa atatctacta actcaaatat tcataattaa actttaaact
     2521 ggataaattt tcttttaaga atgaaagttt atccagtttt gcgattataa taaccagttt
     2581 tacatatcca cttgtaaaat atatctaagc aatatatcat actatataga tttatgagtg
     2641 aaataatatc tgctatttac tgaatatttt tatttttcgt tttttagggg gattagttgt
     2701 gttt
//
LOCUS       DAGSHT010000300         2680 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.306, whole genome shotgun sequence.
ACCESSION   DAGSHT010000300 DAGSHT010000000
VERSION     DAGSHT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.306"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            60..854
                     /locus_tag="MJB72_003534"
     CDS             60..854
                     /locus_tag="MJB72_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264593.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            855..>2680
                     /locus_tag="MJB72_003535"
     CDS             855..>2680
                     /locus_tag="MJB72_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY8264594.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLILLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFA"
BASE COUNT         1022 a          285 c          451 g          922 t
ORIGIN      
        1 gtttatatgt tatatttata taaatataac atatgaacga gaaaggagtg gtttgataaa
       61 tgattgaggt agataaattg tgtaagtctt ttacaagggt tgtaaaagat gataagaata
      121 aaagttcaat aagaaagcta aagaaagtaa aaactaaaaa agaagaattt tttgctgtaa
      181 ataatgtttc atttaaagtt atggaaggtg aaattgttgg aatattaggt ccaaatggtg
      241 caggaaagac tacattactt agaatgttag gtggcatatt aacaccaaca tcaggaagta
      301 taaatatttc tggatatgat tattcaatag atagaaattc agcaaaaaaa gaaataggtt
      361 atctctctgg aaatacaaaa ctatatggaa ggctttctcc gagagaactt ctaacaactt
      421 ttgcaagtct atatgagatg tcaaaagaag atatagaaga atcaattgaa aatattgtaa
      481 aaataatgga tatgagtgaa tttatagata atagaattga aaatctatca actggtcaga
      541 ctcaaagaac atctatagct cgttgtttaa tacattctcc aaaagtatat atttttgatg
      601 agccaacact aggtctggat gttttaagta gtgcatctat tatagatttt atgaagaatg
      661 aaaaactaag gggaaaaact gttctttact caactcatta tatggaagaa gcagaaactt
      721 tatgtgacaa gatttttatg attcacaatg ggaaaataat tgcatcagga acaccacaga
      781 gtttaaaaga ggaatctgga gtaaataatt tgagagatgt attcataaaa cttgccagaa
      841 aggaggacat ttaaatgaga agtaagattg taaaatacat atttaaaaaa gaaatgttag
      901 acatattgag agataaaaag actttattta tgatgattgt tgtgccactt cttttatatc
      961 ctttacttat gttattaatg attcaagtaa tgaatatgtc tgcaagtagt atgaatagta
     1021 aggacattaa tatagctttt aataataaac ctaataaggt tttagtatcc atgattgaag
     1081 atgataattt agaaaaagat actaaaaata cttcaaatga taaaggcaaa ataagagtgt
     1141 tagatgtaga taactataaa aagtcattag aagaaaataa aattgatgct tatataaaaa
     1201 tggaagaaaa aaaatcatct ataaattatc aaatatatat aaattcttct caagaaaatt
     1261 cttctaatgc actaaaaaga attgaaactg ttttggataa atataaaaga tttaccatag
     1321 aaaaaacttt atctgaaaaa ggattagatg tacaagctac tttagaacca ataacatata
     1381 gcactgtaga tgttgcaaag acggaagaag tggcaggata ctttttaggt cagatacttc
     1441 catttatatt aataatagga gttttactag gttcaatata tccagcaata gatgttatgg
     1501 ctggagaaaa ggaaagaggt acattagaaa cattatttac actcccaata tcaaacttag
     1561 aactggttat gggaaaatat atggcagttt ctcttagtgc aatagtaaca gcaattttaa
     1621 acattttatc tatgggactg actatggcat ttattttagt tagtggaggt attagtagcc
     1681 aatttggttt tggaaacttt aattatggag atttagtctt accaattata actactttaa
     1741 tttgcatatg tcttttttca atggtagttt cagcaataag tatgtgtgta tgttcattag
     1801 caaaaagttt taaagatgct caaaactaca taacacctct tatgttaatt attatgatac
     1861 catcatatgt ttctatgata ccaaatatag tattagatag agtaacagct gtaatacctg
     1921 tagtaaatat atctttactt ataaaaagtg tattatcttt taaaagcgat ttaaatttaa
     1981 tagccttagt tttagcatca aatgtagcct ttgttatatt ggcaatcata ctactgtcta
     2041 agatgttcaa ttctgaggaa atactttttg gtaatagtaa gagtttctct ttcttagaga
     2101 aaagaagtaa tataaaaaaa gggtctatac caactgtaag tgatggtgtt attttatatg
     2161 cagtaggatt aattctattg atttatgttg gttctttagt tcaaattaaa tttggtatgg
     2221 caggtattat aatgacacaa ttcatgataa tttctttacc attattattt gcatactata
     2281 ttaaagctga ttttaaagag gtatttagct tgacagttcc taaattaaag catgtgtttg
     2341 ggagcattgt tctttggatt ggtggataca tacttattat gattataaca cagctaattt
     2401 tattcttatt tcctcaaaat atggaagttg ctgaaagttt aaataaggct ctttttatca
     2461 aagatagtgt tttactgaat ttactagcaa tagctttatt accagctata tgtgaagaaa
     2521 tgttttttag gggatttata ttttcttcat ttagtaaatc aaaagataaa aataagtcta
     2581 tcaagctagc tataatatgc agtggggtac tatttggtat tatgcatatg gattttatta
     2641 gaataattcc aacatcaata ttaggtatta tatttgcata
//
LOCUS       DAGSHT010000301         2647 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.307, whole genome shotgun sequence.
ACCESSION   DAGSHT010000301 DAGSHT010000000
VERSION     DAGSHT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.307"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            375..2159
                     /locus_tag="MJB72_003536"
                     /pseudo
     CDS             375..2159
                     /locus_tag="MJB72_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1156 a          358 c          461 g          672 t
ORIGIN      
        1 ttttcatatg gataaaattg gttatttaat atataattac aagtataaat aataaaattt
       61 atttgtaaaa agagtagcac ttttaattct aaaggctact ttttttatac aaaaccctat
      121 tttttataac aattcattaa cataaaaatc aaacttacct taaaatataa atttacaaat
      181 ttttcaatca ataagaaaat ttctttattc cttacataat gactgcaatg taacaaaata
      241 attacaaatt aattatgaaa tcattatagt tagaaacctc tttaaaatta gatattaagt
      301 atcctttaga attagaacgg gaacaaagtt aaaaaattaa gtaatatcaa atggacaagg
      361 gggaagaaaa taaaatgaaa ataccaaaga agattgctgc aatgcttaca gttacaatga
      421 tagctggaag ttcaacagca ggcatagcta gtgcacaaac tgtggcaaca aatttaacag
      481 ggcaagaaag atatgaaact gctgttaaaa taagtcaaga tggatggaaa aatgctgatg
      541 aggtagtaat cgtaaacgac tcatctatag cagatgctct atctgcaaca ccatttgcaa
      601 aagcaaaaaa tgctccaata cttttaacaa gtaaagataa attaaatgat aagacgaaag
      661 cagaaataca aagattaaaa gctaaaaaag tatacttaat aggtggaact tctgtattaa
      721 gtacaaatat agaaaaagaa ataaaaaatt taaaaatatc ttttgagaga atctctggag
      781 ctgagagata tcaaacatcc ttagaattag caaagaaatt agatgctata agtgatgtta
      841 agaaaatagc tgtagttaat ggtgaaaaag gacttgcaga tgctgtaagt gtaggagcac
      901 cagctgcaca aaacaatatg ccagtaatac ttgctgattc taaaaatgga acagcagttg
      961 cagacaaatt tataaaagat gctggaataa ctcaatctta tgtagtaggt ggagagagtt
     1021 caatttctga agctgttaaa aataaactac caaatagtac aagactaggt ggaacagata
     1081 gaaatgatac aaatgcaaaa gtaataaaag aattttataa aaagacagat ttaaaaaatg
     1141 cttatgtaac taaagatggt atgaataaac aagaccaact tatagatgca ctagcagtag
     1201 gtgtattagg tgctaaaaat caatcaccag tagttttagt tggaaagaac ttatcagctt
     1261 cacaaaaatc tctagttaac tctaagagtt ttgataagat aactaaagtt ggtgggaatg
     1321 gtaatgagac agcatttaat gaaatgaagt cattacaaga agtaaaaaca gttgaagcta
     1381 aaacaatttc tgaattaaaa tcagctatag ataaagctac tgctaatgat gttataaact
     1441 ttaagccaac tagtgaggtt aaagaagcat ttacaataca aactgataaa gcagtaacag
     1501 taaacttaaa tggtacatat acaaagactg taactatcaa tatgcctaat ggagatgtaa
     1561 ataactatgc aaaagtggat gatgtagtta tagatgatgt aaaagatgga acttttgtta
     1621 actacggaaa aataactaac ttaaaggtta atgataaaaa tggagccaag atagaaaata
     1681 attctaaagg tgagattggt agtttaacag tagcatctgg agcaagtcaa gtaaaagtga
     1741 ctaatggtgg taagataact acagttacaa ataattctaa agggactact atagataata
     1801 aaggtacaat aagtagtgtt aagggagata attctccaac aataagtgga aatagtccaa
     1861 gttctaactc ttcaggagga agttcatcaa gtggaggctc ttcacatgga ggaggctcat
     1921 cttcaggagg aagttcttct aatcaaacat cagtaaataa tgaagcagca aaaataacaa
     1981 gcgtgcctac accagcaaaa gatgcaacta gattaactat gccatcagta tcaagtggat
     2041 atactatagc aataaaaaca tcaagtaatg agagtgttat aaaaaaagat ggaacaataa
     2101 tacctccaaa tacagcaaca acagtaaaat tagtatttac ggtaacacat acatcaagtg
     2161 gtaaaacagc agatacaaaa gaattatctg taacagtacc agctaaatca acagatgaag
     2221 aattacaagc agcattagac aatgaagtgg caaaaataac aagcgttcct gcaccagcaa
     2281 aagatgcaac taaattaact atgccatcag tatcaagtgg atatactata gcaataaaaa
     2341 catcaagcaa taagagtgtt ataaaggaag atggaacaat aatacctcca aatacagagg
     2401 aaacagtaac attggtattt acagtaacac aagaatcaag tggtaaaaca gcagatacag
     2461 gagaaataga tgtagtagta ccagccaaat caacagatgg ggaaatacaa gcagaagtac
     2521 aagctgaggc ggataaaata acaagtgtaa ctcagccaac acaagatgca acaacactaa
     2581 ctatgccaac agtagcaagt ggatacacaa taaagataaa atcatcaaca aatgaaagtg
     2641 ttatagc
//
LOCUS       DAGSHT010000302         2643 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.308, whole genome shotgun sequence.
ACCESSION   DAGSHT010000302 DAGSHT010000000
VERSION     DAGSHT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.308"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..694
                     /locus_tag="MJB72_003537"
     CDS             <1..694
                     /locus_tag="MJB72_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264595.1"
                     /translation="IYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNIKECD
                     LVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTKPFSP
                     MSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNIDLTP
                     NEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNILIET
                     VWGFGFRLKEKS"
     gene            696..2183
                     /locus_tag="MJB72_003538"
     CDS             696..2183
                     /locus_tag="MJB72_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8264596.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
BASE COUNT         1072 a          243 c          472 g          856 t
ORIGIN      
        1 aatttatata gcagatgatg aagataatat aagaaatttg gtaaagacat tcttaaaaaa
       61 tgaaggtcat gatgttatgg attttaaaac gggtgatgaa cttttagaac aatttaatat
      121 aaaggagtgt gacttagtaa tactagatat aatgatgcct ggctcaagtg gttttgaagt
      181 atgtacaaaa cttcgtgaga aaagtacagt cccaataatc atgcttactg caagagatac
      241 agatatagat tatattacag ggataacctt aggtagtgat gattatttta caaaaccatt
      301 tagcccaatg tctttggtta tgagagtaaa atctatattt agaaggatag agtttgaaaa
      361 gaaacaaaat tatgataaat atagtaactc gatagatatg gaattaaaat ttggtgatgt
      421 aataataaat aaaaagaata aaatcgttac aagtaaaaat gtaaatatag accttacacc
      481 aaatgaatat aacctattga cttatttgtt tgaaaatata gatagagcag tttccagaga
      541 tgaattatta aacaaaatat ggggatacga tatagaagtt gagacaaggg ctgctgatga
      601 tacagtgaag agattacgta aaaaaatttt aaatacaaat attttaatag aaactgtatg
      661 ggggtttgga tttaggttga aggagaaaag ttaagatgaa gaatggaaat gacaagaata
      721 gaattggaaa gattaaatcc aaatttaaat taaagttctc aaagaaaaat ataagcataa
      781 aaactaggat tttgttgagt atactttgtt tgataattat agtatttatg ataatattag
      841 tatcttttaa tgtatttctt gatacatata taaaaacaac tgcgaatgaa gaattgacaa
      901 agtctacaga aatagtagaa catatggata gtaattttag acctattaag ccaccacaag
      961 atgatggaaa gcttcctccc aaagtattat ctaattttat gagaaatgta caagataaag
     1021 ttaggatggc agaaacacaa tcagatgctg atgctatggt agttgattct aagtataacc
     1081 tgatatttcc aacaagagag gatgatttct taaaaaatgt tgatgagatg gagctgatta
     1141 gggagtgtat tcaaaatgaa aaattagatt taaattcaga tgcaaataca cggatatcta
     1201 ccagaaataa ggattattat atttcaactg taaaaattac tagtatatat ggtgaacaag
     1261 acaagtatct tatacttttt atagacatat caaaaactct aaatctagca caaaagataa
     1321 atattgtatt aatatctgta atgtgttttg ctggaatatt agcaatattt acagctgtga
     1381 ttttatctga gaaaattgct aaaccgataa aagagttgtg tgagtttgca aaaaaaatgg
     1441 gtcaaggaga tttcaaaaga acctactttg acttttcaga taaagaatta gtagagctct
     1501 caactgttat gaataagagt gctgagtatc tggataagta tgataatgaa caaaaaatat
     1561 tttttcaaaa tgcatctcat gaattaagga caccacttat gtctattaaa ggttatgcag
     1621 aagctataaa atataatgtt atagatagta aacatgcaag tgatataatt cttgaagaaa
     1681 gtgataggtt aagtgatatg gttgaagatt tattatacat ttctaaaata gataatataa
     1741 ctaaagattt tgaacttgta gaatgtgatt taagagaagt actttcaaat tgtggagcta
     1801 ggcaaaatgt aagagctata aataagggga taaaatttat ttatgaattt gatgaagaaa
     1861 tggttttatt tgaatgtgat gaaaaaaaca tttccaaagc atttatgaat cttatagaaa
     1921 atgctttgag atacgctaag agtgaaataa aaatagtgtg taagtataat caaaaaaata
     1981 tagttgtgat aatagaagat gatggaattg gaatcaagaa agaagattta cctcatgttt
     2041 ttgaaagatt ctataaggga gtaggaggaa atcatggaat tggattatca atagttaaat
     2101 ctattgtaaa taagcatggt gggcgtatat acgtagaaaa tggcaaaaaa ggagctaaat
     2161 ttactatatt atttaaattg taaaaaaatt agagaaaata tagagatgtt tgaagttgtt
     2221 ttttatatat atctgttgta tactgttgaa aaaagaacaa tattaagctg acatatatta
     2281 cgatagttga catttaaagt aaatgcttaa atatagatac gaaaggaaaa gtaaaatctg
     2341 aagtgtattt aggtttaagt aagcttctta ttttataaaa aatatgtatg aaagaacagc
     2401 tgatttttaa tattacaaat ttagtaacgg ttgtatttga agcttttgtc atccatatgt
     2461 ttttatcaga ctttttaggc cagaaagaag ggtatataaa agttgtaaga tatgctaaac
     2521 tgggttttat aatatgttta ggattctgta atttaataac tttaaaccct aaaatcacaa
     2581 tgcctttaat tttcatcctt attttttcaa catcattttt atataaagga aatttaaaga
     2641 cac
//
LOCUS       DAGSHT010000303         2625 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.309, whole genome shotgun sequence.
ACCESSION   DAGSHT010000303 DAGSHT010000000
VERSION     DAGSHT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.309"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1783
                     /locus_tag="MJB72_003539"
     CDS             <1..1783
                     /locus_tag="MJB72_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264597.1"
                     /translation="LCSLILYANNFLVKKRNKELGIYMTLGMEKVKISKILVFETIIV
                     GIVSLVSGLILGLLVSQGLSILLLKLFEFNMSEYNFVISISSIIKTIVYFGIMFLLVM
                     IFNTYIISKYKIIDLLTIGRKTEDIKFKSPFVYTITFILCIISLIIAYSMVLKVGLNP
                     KNPKFIMSIVLGVIGTILFFYSLAGFALYIVKKNKNIYFKGLNIFVVKQINSKINTNF
                     ISISIICLMLFLTIGILSTGFGFKSALNAGLKGATPFDASATMIVHEKCKVKNIKESF
                     EKVGIKFNKGDKVAYYDEYINGNKLSDVMQGQYSGRVDFEVAYIKSSQYNEIRKLRGE
                     KSITLSKDEVLITSNATEVVPSIEEYMKNNKIIKIDNKAYNIKNKKVIKDNLRTDFTE
                     TNALTVVVNDKLCDSMEISSSIVNINFASDNNEKSQENFDKRLKSKYSSTSFYEVGTV
                     SGGSRNAMYNDNNGMTSTILFVGIYLGIVFLISSMAVLALQQLSEASDSIDRYVSLKK
                     LGASKNSISKTIFTQTLMYFSIPVGLALVHSVVGIQVANNFISRLNKPDIGASSLMTV
                     GILMLVYAVYFYTTYQGYKNIVIKKLN"
     gene            1860..2531
                     /locus_tag="MJB72_003540"
     CDS             1860..2531
                     /locus_tag="MJB72_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264598.1"
                     /translation="MEKIIIVEDEEVIRDELKSFLERYGYEVMAPTEFDNIVEFILSE
                     KPNLVLLDINLPIYDGYYICRVLRKESNIPIIIVTSRNTDMDELMSMNLGADDFVTKP
                     YKTQILLARIVALLKRSGLNSNVNNTLIFKGIELNLFNATLRYKDKEIELTKNEFKIL
                     SYLVENQGSIVSRDSFMEYLWHTNWFVDDRTLTVNITRLRKKLDEIGIENLIETRRGL
                     GYIIS"
BASE COUNT         1064 a          244 c          429 g          888 t
ORIGIN      
        1 tttatgcagt ttaattcttt atgctaataa cttcttagtc aaaaaaagga ataaggaact
       61 tggaatttat atgactttag ggatggaaaa agttaaaata tcgaaaatat tagtttttga
      121 aactataata gttggaatag tttctttagt aagtggacta atacttgggt tattagtatc
      181 tcaagggtta tcaatattat tattaaagtt atttgaattt aatatgagcg aatacaattt
      241 tgtaatatcg atatcatcaa ttattaaaac tattgtctat tttggaataa tgtttttact
      301 tgtgatgata ttcaatactt atataatttc taaatataaa ataatagatt tattaacaat
      361 aggaagaaaa acagaggata taaaatttaa aagtccattt gtatatacaa ttacatttat
      421 attatgcatt atttccttaa ttattgcata tagcatggtt ttaaaagttg gattaaatcc
      481 taaaaatcct aagtttatta tgtcaatagt attaggtgta attggaacta tattattctt
      541 ctatagttta gcaggctttg cattatatat tgttaagaag aataagaaca tttactttaa
      601 gggattaaac atatttgtag taaagcaaat aaatagtaag attaatacaa actttatatc
      661 aatatctatt atttgtttaa tgttattttt aacaatagga atactatcaa cagggtttgg
      721 ttttaaatca gctttaaatg caggactaaa aggtgcaact ccatttgatg caagtgcaac
      781 catgattgta catgaaaaat gtaaagtgaa gaatataaaa gaatcttttg aaaaggttgg
      841 aattaagttt aataaaggtg ataaagttgc ttactatgat gaatatataa atggtaataa
      901 actttcagat gttatgcaag gacaatattc tggtagagtg gactttgaag tagcatatat
      961 aaaatcttca cagtataatg aaataagaaa gttacgaggt gaaaagtcaa taactttatc
     1021 aaaagatgaa gtattaataa catctaatgc tacagaggta gtaccttcga tagaagaata
     1081 catgaaaaat aataaaatta taaaaataga taataaagca tataatataa aaaataaaaa
     1141 agtaataaaa gataatttaa gaactgattt tacagaaaca aatgctctta ctgtagttgt
     1201 aaatgacaaa ttgtgtgata gcatggagat atcttcaagt atagtaaata taaacttcgc
     1261 ttcagataat aatgaaaagt cacaagaaaa ttttgataaa aggctaaaat caaagtatag
     1321 ttctacaagt ttttatgaag ttggaactgt atcaggtgga tcaagaaatg ctatgtataa
     1381 tgataataat ggtatgactt ctacaatact atttgttgga atctatttag gaatagtatt
     1441 tttaataagt agtatggcag tccttgcact tcagcaatta agtgaggcta gtgatagtat
     1501 agatagatat gtatccttaa aaaaactagg agcaagtaaa aatagtataa gtaaaactat
     1561 atttacacaa actctaatgt actttagcat acctgttgga ctagctttag ttcactcagt
     1621 agtaggaata caagtggcta ataattttat atcaaggctt aataagccag atataggagc
     1681 ttcatcactt atgacagtag gaattttaat gttagtatat gcagtgtatt tttatacaac
     1741 ttatcaaggg tataaaaata tagttataaa aaagcttaat taatacaact aactaaaatc
     1801 tgtttacagt aaaaatcata ctgtaaatag attttttgaa aataaagggt gaaaaattaa
     1861 tggaaaaaat aattatagtg gaagatgaag aagtaatcag agatgaatta aaaagctttt
     1921 tagagaggta tggatatgaa gttatggctc ctactgaatt tgataatata gtagagttta
     1981 tattaagtga aaaacctaat ttagtattac ttgatataaa cttgccaata tatgatggat
     2041 attatatatg tagagtatta cgtaaagaat cgaatatacc aataataatt gtaacaagta
     2101 gaaacactga tatggatgaa ctaatgagca tgaatttagg agcagatgat tttgtgacaa
     2161 agccatataa aacacaaata cttttagcaa ggattgttgc attgttaaaa agaagtggat
     2221 taaattcgaa tgtaaataat actttaatat ttaagggtat tgaattaaat ttatttaatg
     2281 caacactcag atataaagat aaagaaatag agttaactaa aaatgaattt aaaatactat
     2341 cttatttagt ggagaatcaa ggttcaatag tatctagaga ttcatttatg gagtatctat
     2401 ggcacacaaa ttggtttgta gacgatagaa ctcttactgt caatataaca aggctacgta
     2461 aaaagctaga tgaaataggc atagaaaatt tgatagagac aagacgtggt ttagggtata
     2521 taatatcatg agattaagag agtatataga tgataaagtt gtttttctag gtactaattt
     2581 aattatattt ttaattgcat caggtatcat gctttatagt aaaat
//
LOCUS       DAGSHT010000304         2617 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.310, whole genome shotgun sequence.
ACCESSION   DAGSHT010000304 DAGSHT010000000
VERSION     DAGSHT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.310"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..367)
                     /locus_tag="MJB72_003541"
     CDS             complement(<1..367)
                     /locus_tag="MJB72_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264599.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDD"
     gene            complement(381..1316)
                     /locus_tag="MJB72_003542"
     CDS             complement(381..1316)
                     /locus_tag="MJB72_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264600.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTSFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(1445..2092)
                     /locus_tag="MJB72_003543"
     CDS             complement(1445..2092)
                     /locus_tag="MJB72_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264601.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(2085..>2617)
                     /locus_tag="MJB72_003544"
     CDS             complement(2085..>2617)
                     /locus_tag="MJB72_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264602.1"
                     /translation="NAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYE
                     NYQSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSITLYRLIQESLSNSSRHGK
                     ATEIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGF
                     TIRASFPKFSGGDFFE"
BASE COUNT          874 a          385 c          297 g         1061 t
ORIGIN      
        1 tatcatcacg agttccaagt ttttctatgt ttagagtatt tgatttttca tttaataaac
       61 tactctcata gcctttaggt atagtaagct ttaattctgc cttagaatca tcttttttaa
      121 ctgttaatat ataagataaa tcatttttca aatactttgt caaatcattt gataatcttg
      181 tgttatcttt gtcatatata acaatggggg tagattctgt ttttataggg ttttgaaaca
      241 tgctttctgt aaccatccca agaaaccaag ataaaatcag aggcaaagct aaaaaatata
      301 ttaaattaag tataaaggag ctttttaaac tctttagcat agtttttaca ctcatgtata
      361 atttcataaa atcacctcca ttagtccctt aaattttttc ctgttagatt taaaaatacg
      421 tcttctaggc taggttcttc ataagaaatt ttcattattt ttgaattgtt tttgtcaatg
      481 acagagagta tatctgtaag ttgtaatttt ttatctatat ccaattctaa taatgaatta
      541 ttttctaaaa cagatataac acctttagta ttttttaaat caaagataag ttgggcattt
      601 atatttttta tttccataat taattttgta tttgtagata cgagtgattt taaataatca
      661 ttatttccaa aagcaatttc tttaccttca tccataataa aaattttaga acaaaggtgc
      721 tcaacttctt ccatataatg ggaagtatat attacagtag tttcatgttt tttagatata
      781 tcttttataa aattgaaaat atgatttctt gattgggggt ctacacctac agttggttca
      841 tccaatatta atattttagg atgatttaag agtgctattc caatatttaa tctcctttgc
      901 ataccaccag ataatttttt gacctttttc tttttgactt cagtaagacc tgtaacttct
      961 aatgcttcaa tgattctttc tttaagtagt tttcccttta aaccgtagaa agctccaaag
     1021 tactctaagt tatcaaaaga agtaagagtt tctataattg ctaattcttg tggaactaac
     1081 cctattatag actttgcttt tatataatct tttgataaat ctaaaccatt gatttgaata
     1141 ctaccactat ttggtaacat aagatttgta attatattta taagtgtaga tttacctgca
     1201 ccatttgggc caataagtcc aaatatctca ccttttttaa cagaaaagct gatgttatca
     1261 agtactaatt tatcattaaa tctttttgtt atattattta ctctgactat atccatagta
     1321 atccctccaa aataattttg tataagttaa aaagctataa tattataata aagaattgtg
     1381 aattataatt ctattactat aagtcactaa aaaaagtgac ttaagtcact ttatattttt
     1441 agatttattc tattacaatt ttatttttga aagcaaagat tgctagttga gttctgtcac
     1501 gaagttctag tttagctaaa atattagtta tgttattctt tattgtacct tcagataaga
     1561 atagttcttg acttatttct ttattattaa gtccatttgc aattagtcta attatagata
     1621 tttctttttc tgttaaattg tacatgtcat aaagatgctc ttgtttaacc gttttttcag
     1681 aggtcatgat tttttcagct acacttttat caagcacaat attgccaaaa aatgaagaac
     1741 gaatcccttc atgcaatact tcatcagagc tatcttttaa aaggtatcct gaagcaccaa
     1801 acttcatagc atcttttata tattcatcat cattaaaagt tgttaaaatt aataatgtta
     1861 tagaattatt atattccttt ataagccttg tacctagaac cccatcacat actttcattc
     1921 gtatatccat aagaactatg tctacatcat tcattttaca aaattctaaa gcttcatatc
     1981 cattagaagc atctcctaca atttctaaat cattataaga tgataatata attttaagac
     2041 cttttctaat taatttttcg tcatctacta taagaacttt aattttattc aaaaaaatca
     2101 ccccctgaaa atttaggaaa agaagctcta attgtaaaac cattattaga attatcaaat
     2161 tcaactttac catttaattc atatattctc tcattgatac tattaagtcc atttcctttt
     2221 tttatattgc cacatcctat accattatca ctaatagtaa ttataagatt tgaagtattg
     2281 aatgtaataa aaattctgat ttcagttgcc tttccatgcc tagatgagtt ggaaagggat
     2341 tcctgtataa gtctatataa agttatagat tgatttctgg ataagttcca agtatttttt
     2401 gaaatagtta atttaacatt aatatttgta agcttaataa agcttttaac taattctttg
     2461 attttaaata aactttggta attttcatat tcaacaggtt ttaactcaga tatagccctt
     2521 cttacatctt gaaaactttc agttacaaat tctctaagtt catgtactaa gtcataaatt
     2581 agaggtttgt cttttaagag tttttctata gcattta
//
LOCUS       DAGSHT010000305         2572 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.311, whole genome shotgun sequence.
ACCESSION   DAGSHT010000305 DAGSHT010000000
VERSION     DAGSHT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.311"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..672
                     /locus_tag="MJB72_003545"
     CDS             <1..672
                     /locus_tag="MJB72_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8264603.1"
                     /translation="IDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALI
                     YYSVINYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVF
                     AICMAFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSI
                     STVISLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSN
                     LNKVA"
     gene            complement(790..1458)
                     /locus_tag="MJB72_003546"
     CDS             complement(790..1458)
                     /locus_tag="MJB72_003546"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBY8264604.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(1525..1953)
                     /locus_tag="MJB72_003547"
     CDS             complement(1525..1953)
                     /locus_tag="MJB72_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY8264605.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            2060..>2572
                     /locus_tag="MJB72_003548"
     CDS             2060..>2572
                     /locus_tag="MJB72_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBY8264606.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRF"
BASE COUNT          861 a          405 c          294 g         1012 t
ORIGIN      
        1 attgatttta agacacttag aaaaattgta tttataggtt ttcctgattc aactactgaa
       61 ttaagttgtg gtatagttgt tttacttttt aacttaagtc ttactaaata tattggagaa
      121 aatgctctta tttattatag tgtcataaat tatataaata cattagtact tatgactatg
      181 atgggtataa ctcaaggtat gcaaccactt actagctttt attatggtgc aggaaatata
      241 gataatgtaa aaaaattatt aaaaatgggg ataaaagcca caataatagc atcagttgct
      301 gtatttgcaa tttgtatggc tttttcagga ccaatagtct ctttatttat acatccagaa
      361 gaaacaatgc tattcaatga aggagttcgt gtatttaaaa tattctctat atctttctta
      421 ttggtaggaa ttaatgtaat aatttctgga ttctttgtat cagtagaaaa accatcaatt
      481 tctactgtta tatctcttgg tagagggctt gtaatagttg ttttaagtct aatttcaatg
      541 atactaatat ttggtggaca aggaatatgg atgactacta ttgtttctga gtttatatgt
      601 cttattttat cattagtttt tctaaaaaag aatttttcta cattagactc taatctaaat
      661 aaggttgctt agatgataag agtgacaata tatgaaaata tctaaataaa aaatacctat
      721 tatacaatct tgcaaatatt aaacctttgc aaaaattagg attatataat aggtatttta
      781 tttatgtaat tattttattt ctaaatatcc atagccatct tttattaaag ttccttttac
      841 ttcatattct acaggttttt taaatattgg accatctacg acaaatgtat gatactctcc
      901 atgttctcca catgggtctg caccagcatt ttttatcttc tccacaagtt ctctagttaa
      961 tttttttcct aaaaattcct ctcctaagtg ctttaagttt acttttgtta caacactaca
     1021 aaatccactg tctataaatt catatactaa ctcctctcta ctctcttgcc ataatggtaa
     1081 atctactttc ataccaactg cttcacatct atcagtatcc cattttctat gactttctat
     1141 atctatatct ccatatgcac atatagttgc tcccatgttt ttagctttta ttaaagccct
     1201 ctcaaagctc tcttcatatc cttctacacc acactcagca actaataaag gtaatcctat
     1261 attgctactt acttgcttaa gcatattata ttctaaatta tgtgtccatg actttccatg
     1321 ttcttcactt atagttgtca agagtgccac aggcttatat ccttttttca acattcgatt
     1381 taatgctaat atactatcct taccaccact aaatgacatt acaaattttt catcagccaa
     1441 ttttaacacc tctttcatct aactttgttt tattaacata attataccaa atttttgagt
     1501 tgcatagcaa attatattaa attgttaatt cactataaag aatgtcccta atattaaaag
     1561 tataacccct atgtatttgt ataaatgcat tgtttcttgt cccaaaatta cgctatcaat
     1621 aaaaaatcca actaaaactt gggatacaag agttaatata agtgtatttg atactcctaa
     1681 atttggaatt gcctttactg taaaatatat tattagacct ccaaatactc ctcctaataa
     1741 caacttataa ttaaaaccat ttatagatac tagacacttt aagtcacctg taactattat
     1801 acttgccaaa aaaaatattg ctcctacagt taagcttata aaggtggcca ctaatgctgt
     1861 tgtacttttt cctagctctc catttataaa agcttcaaga gctgtagaac cacctgctaa
     1921 taatgcaaat attattgccc ataattttgt catggacaaa cctccttcta tataattcta
     1981 ttaatagaag tttatgatac aacctattaa ttaattacta agtatagttt aatttttata
     2041 cactaggctt tatttggata tgaatttctg ttataattgt tctatcgatt ttggaggtga
     2101 ttttatgtac acaaatttta atatcgattt taataacttt aataaaaaag aaaatgccat
     2161 taaatttatg ttaatgggag tattactcat tatattagga ttattatgcc ttacatttaa
     2221 gacattaggt ataaaattaa tttcttggac ttttggtatt gcacttttat tctttgcata
     2281 tttaaattta aaaaatatta atgaactaaa aagatatgct actaaagaag aaattaaacc
     2341 ctctattaat atccaatggg ttctaataat tgcatgtata ttactttttg tatttcctca
     2401 aaaaatacaa agcatatttt cattattttt aggattttat ttaatcttta atcaactagt
     2461 agcacttgta aatagtaaga ataatcctta ttctaaattt acaacttgga atatagttaa
     2521 gattctattt ggtatatgcc ttatattatc tccacttttt ttatctagat tt
//
LOCUS       DAGSHT010000306         2569 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.312, whole genome shotgun sequence.
ACCESSION   DAGSHT010000306 DAGSHT010000000
VERSION     DAGSHT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.312"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(299..2392)
                     /locus_tag="MJB72_003549"
     CDS             complement(299..2392)
                     /locus_tag="MJB72_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264607.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT          870 a          418 c          249 g         1032 t
ORIGIN      
        1 taaataaata tctatctgaa caagcatagt ttaattaaaa acttttgtat tcccttaaaa
       61 ctgtatctac ctcttattat tttatttatg atatattatt tttaatctaa aaatagatat
      121 ctatttttat tatggttatc cctttttaaa atactttaat catatactat tcccaattta
      181 acatccattg aaatgctatt ttcttatatt gcaatttata attgatatat cttcaatata
      241 ataatttaat gtatttttga ttttaaagct tttcagtgat attattgata aattagaatt
      301 aattatctag ctttaaaagg tcaaagattt ctgttgctgt ctgcggtttt ccaaataaat
      361 atccttgtat gtaatctggc tttatttgtt taactaaatc atactcttct tgtgtttcta
      421 caccttctaa acatactttt attcctacgt catgacaaat tgcaacaata aaatgtataa
      481 atgtagcatc aaacttactc tttaatatat ctttaacaaa tactctatca attttcacta
      541 tatctattgg agaaaatttt aaaacttcta gagacgagta tcccgtacca aaatcatcca
      601 tagcaatata aattcctaaa tcttgtaaag ccttaaattt aaactgtaaa atactcatat
      661 tttgtactgt atgactttct gtcaattcta aaacaagatt ttgataagga aaattttcat
      721 catctataat ttttactata tcttcaataa atgtgtcttc taatatttga acagctgaaa
      781 cgtttacact cactttaaaa aaaggatagt aatcaatcca tttcctaaaa gtacgtaatg
      841 ccattcgaag cacccaagcc cctacaatat ttatcatatc attttcttct aatattggaa
      901 taaattccaa aggagaaatt gctttgcatt tgtcattagt ccaccttaaa agaacttcta
      961 cacctattat tttatgactt tctgtatcta cttgtggctg gaatcgaaga ctaaaccccc
     1021 taaaatcatc attaattgat gcctttaaat cccgcatcat ttctaatgaa tacattttat
     1081 tttttaatat ttcttgggag aaaaaaacaa gcctgttttt accatgctct ttcgcatatt
     1141 gtaaagagta actagcacac ttataaagtt cttttactgt atctccatgt tttggatata
     1201 ttacacaacc agcagaaatt gttatattta atccatattt tctccatagt tgaaggtgta
     1261 taatcatatt ctgtatttga ttatatagag ttagaatttc attttcttca acattatcaa
     1321 caagaatgcc catttcgtcc ccatcaagtt tataaagttc agcattatct ggtaaaatag
     1381 attgtataga ctgtgctagt gtttttatta tattatctcc aaaatcccta tcatacatct
     1441 cattaacatt tttaaaatcg tcaatattta ataatactat gcataatttt tcaatcatag
     1501 gatttgaaat tttatcttca aatacactca taaattcatg ataattcagt aactgtgtca
     1561 atgaatcaat cttattttgt ttacctaatt gcgtcattat tcctgcaaaa atacttggtt
     1621 ctccaaattc atcacgcatt agttgacctc tacatcttaa ccatatatat tctccactac
     1681 gatttttagc cctaaattcc actgtatggt aatccatctg gtttttacct atttcagtgt
     1741 ttgacttata aaagcgattc cagtcttctg gatgtacaat ttttttccag taaacaagcg
     1801 gattttcaac aatttctcct ggtaagtcaa ataattcaac ttgtgatggt gaatatcgaa
     1861 atacgcctgt tttcatattg caaatgtata tatattcatc tgtacctcta atcaaagcat
     1921 cataaagcaa tgtagaatta taatcaaaat tgtctcttga aaaattaaaa actccttttt
     1981 cttctttatt atttgatatt tcatgaaatt cattaatctt aattggttta tttaagcttg
     2041 ttgagtttgc aatatctatt gattgatgca tatgaacaac tgtgccatct tgccatgtaa
     2101 ttaaactatc ataattttca aaaacacgat ttaacttatt acatttttca taccacttta
     2161 ttaatactcc ttttttatca ttctttaata attctaaaac tttacaaaaa ctacaagtag
     2221 aatttttttc aggatataat acttgccaac aaacttttcc ttctggatct aaaatattat
     2281 attcttcttt catttttttg ttcataaaaa taatttcatt agtatgaata tttgtaacat
     2341 atatattaat ctgtagttga tttaatataa cttcaaaatt atgtttgttc atatacttat
     2401 ctcctccata gttactgctc tttataacaa cattgttaaa attattcttt ttagaagatt
     2461 tacttataac ttatacatat acactaaata ccttaattta attagaaagt ttattacttc
     2521 atattttaaa ttataattga taaaaaaaaa tacagcaata tagtattac
//
LOCUS       DAGSHT010000307         2550 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.313, whole genome shotgun sequence.
ACCESSION   DAGSHT010000307 DAGSHT010000000
VERSION     DAGSHT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.313"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(319..1704)
                     /locus_tag="MJB72_003550"
     CDS             complement(319..1704)
                     /locus_tag="MJB72_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY8264608.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(1812..>2550)
                     /locus_tag="MJB72_003551"
     CDS             complement(1812..>2550)
                     /locus_tag="MJB72_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY8264609.1"
                     /translation="KKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILY
                     LLKKYFHELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKN
                     IILKKRLNSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNK
                     NGETYGTHIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRK
                     RNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT          835 a          375 c          312 g         1028 t
ORIGIN      
        1 gaagcaataa agctttcttt taattgttga gttctttcca caataaatca ctccttttgt
       61 attaaatgta ttaaagtatt cgtaaaacag aaaaatagat taattgtata attgcaatta
      121 taattaaaaa taaatttgga ttaatatata aaatactata caacaaatag ataatcttaa
      181 acggttatta aattttatct atttgttatt atgatgtaat aatacatatt aatctctata
      241 agttaattac tatctggtgt aattttattt taacaacaaa aaaaaataca cacaattaaa
      301 atattgtgtg caatacaatt attttttctc ttctaatagt ctttttaata cgctcaaacc
      361 agctttaagt tcttctgtac tttctgtagc acatacagct attcttattg cagaaattgg
      421 tttttctttt ccaacagcaa aacgttcaga agcatagacc tgtacacctt ctttaagtgc
      481 ttgtatctca aatttttctg ccattattcc atcaggtaga atcaaccatc taaatataca
      541 ttcttcattt cccaaaagat tatatccaga taaatattga ttaataatct tgtttcttct
      601 tcttgcagat ttacggcgtg cattaattaa tttatcggct tcacctgatg taatcatgcg
      661 ataagcaatt tctgttaaaa aataagattg agatagatta atgttgtata atgcgcttga
      721 taatgcctct cgatactgtt taggtgtaga gatatatgct agccgtaaac taggtgcaat
      781 aatttttgat aaactcgtaa tataaattgt ttgattgggc agataggaag ccactggatt
      841 taaatgtgtt tcattaagta aactatgaat agcatcctca ataacaatta aattgtattt
      901 agatgcaata tttgcaatca tttttcgtcc attttgtgac attatatgtg tagttggatt
      961 ttgataatct ggtattatgt atagaccttt aatattttca tttttacaag catataatag
     1021 tccttcttca ctaatctcat tatcttcttg tttaattgga attagttgaa tcccaagcat
     1081 tttagctgct gtctttattc caggataagt taatgggtct acacctattc tatctccatg
     1141 ttgaaacaaa ccagctaaaa tagcaacaat tgcattttgt ccaccacttg caggaagtaa
     1201 gctatctgat gtagtttcaa gcccagcttt ataaattaat ttggcaatag cttctttttg
     1261 ccatttagca ccatctcttt gtccatattg gaatatggtt ttaaaaccta tttccttaat
     1321 tatgtgttta aacaaagtat tcatttcttc tagcgtgaaa tctggattca ttgtgcccat
     1381 ttcaataaat gtaattttat tattactgga catcaagaat agatttgaac gtgtatcata
     1441 agatacaaaa gttccacttc ctgtaactcc acttattaga cctttttttt cacaaatttt
     1501 aaaagctcta gagatagtac tcacattaac atctaagaaa tctgccaatt cacgttgggg
     1561 tggtaattta gtacctggta ataaaaatcc attgttgata tcttgttcta gttgttctgc
     1621 aagcgcctta tataatattt gcccttttga aggtctcttt ggtttccagt tcataggata
     1681 attttcaaaa gaattaattg gcataagttt tcacctcaca ttattaattt gattttttct
     1741 ttatctaaaa caataaattc taaagataca aataaaaact gataggaaat aattctatca
     1801 gtttttgtct tttaattgat ttcaaatctt cctataattt ttataccttc tttatcataa
     1861 agagggattg tttttttaaa tatattaaaa tttctttttc ttgtatataa aacagcttct
     1921 tctggtgtct ttggttgatt atcacaatcg tacaaatctc ttttttttac gtagccgact
     1981 tttttacctc tacctattgt ttgtataagt tctggttcaa tggaatttcc atttgagtct
     2041 attatatgtg taccataggt ttctccattt ttattataat taatcttacc aatagtatat
     2101 tctacatctt cattttgttt aaaattggag ttgatagaaa atattgaccc tattattata
     2161 aaggttaata aaaacttaag acctaaatta attttctttt tatcttgatt aaacatagaa
     2221 tttaacctct tttttaaaat aatatttttt ttatttagaa actgagatac gaatgatgaa
     2281 tacttcaaag atttttgata ctttattgta tctaatatta tatatgcata ctctttaatt
     2341 tcactcgtgg tacacttgtc aatcacaatt tcatcacaag ataattcaca taattcatga
     2401 aaatattttt ttaataagta taaaattgga ttgaaccaat atatgataca aattatacta
     2461 attagaagct taaataagtt atcttttctt ttataatgaa ttaattcatg tttaaagatg
     2521 aatcttaact ctttattatt gtactttttt
//
LOCUS       DAGSHT010000308         2550 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.314, whole genome shotgun sequence.
ACCESSION   DAGSHT010000308 DAGSHT010000000
VERSION     DAGSHT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.314"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(473..1024)
                     /locus_tag="MJB72_003552"
     CDS             complement(473..1024)
                     /locus_tag="MJB72_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264610.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1215..2177
                     /locus_tag="MJB72_003553"
     CDS             1215..2177
                     /locus_tag="MJB72_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBY8264611.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          993 a          271 c          419 g          867 t
ORIGIN      
        1 gtgcatatag tggtaaaaca aaagtttcat ataaatgaag aaaaaaatat aaaataattt
       61 atttaactac ttaaaaataa actaaaaggg ataataaaat agcttttagg ttatggtaat
      121 aaaggtattt aaaaagagtg cataagcact ccaattgtga gaaatacata cacaaatcat
      181 tattacaaca atgaaaaatg tacatataag agacgttttg tatttgtttt aagtgataat
      241 attttaatac taactaaata attatcacaa tagtaaaaaa gaaaaaggag tattttgtac
      301 tccatttgtc aaaaatataa aacttttata taagcttatt ataacataat tgataaaagt
      361 gtaaagatat ataatgaatt tataaagaaa aataaaataa aaaagataaa tcctaatcaa
      421 aggatttatc tttttgtata gaaaataatt attctattat taaatggcat ggctaaactg
      481 gataataatt tattattact gctttcaatg ttttttctcc ttgattatgg tatgtatgaa
      541 ttgattcagc tgaaaaacaa atagagtcat tttctctaag ttgataaata gagtcattta
      601 cttctaattt taattgacct tctataatca taatatattc ctgtgatttt tcagaatgac
      661 caacagatgt ataactatga ccttcttcta attccatttg aaataattcg aagtttctag
      721 ttggtgtatt gggataataa caataaagac gataatcttt attttctgaa acttgtacat
      781 ctatatcagt ttttgataca ataaaagttt cattttgagg ttgttccagt attgcagtat
      841 atggaacatt tagtccactt gcaattttcc aaattgtgtt tactgttggg tttgagtctc
      901 ccttttcaat ttgcgaaagc attactttac ttacgccaga taattcagca agttgaccta
      961 gacttaagtt acgttcatta cgtaatctat ttaaattttt agcaataaca gtattaatac
     1021 tcatagtagc tccttaaaat gtaaattatt gacaaatata atttacgaat gtataattta
     1081 tttaacatta tttataattt acaaatgtat aatataatga acgttaaata cactgtacat
     1141 ccgtaatata aattatactg cataaagagt tgtttttcaa cggatgatag ttgagagatt
     1201 aagggggaat tgtaatgcgt gtacttaata atgttacaga ggctataggc aatactcata
     1261 tgttacattt atccaaaata gcagatgaat ttggagttca aggtaatatt tatgcaaaga
     1321 tggaatactt aaatccggga tttagtaaaa aggaccgtgt agcattacaa atgattaccg
     1381 aagcagaaaa tgcaggttta ttagttccag gtcaagcagt tgtggaatta acaagtggta
     1441 atactggaac aggactagca atagcttgtg catgtaaagg atataaattt atagcgtgta
     1501 tgtcaaaagg aaattctatg gaaagagcaa gaatgatgaa agctttaggt gctgaagtag
     1561 ttcttgtaga ccaaatgcct aattcagttc atggacaggt atctggtgaa gatttggcac
     1621 ttgtagaaat tgaggctcaa aaaatagcaa aagaaagaaa tgcatttaga gcagaccaat
     1681 ttaatttaga agcttgtaat catgcacatg aaaagtatac agcagaagaa atgtgggaac
     1741 aattggatgg aaatattgat gtatttgtag attttcttgg aagtggtagt acttttgcag
     1801 gatgttcaaa aacacttaaa aaacataatt cttctatcaa atgttatgtt gttgaacctg
     1861 aaacagcagc aatttattca ggtaaagaaa taactgacca agggcataaa atacaaggtg
     1921 gaggatattc tatggatttg ccattagtag acaagaaact tattgatggt tatattcaag
     1981 ttacagacga agaggcaact gatgtagcaa gaaaattagc taaattagaa ggtgtatttg
     2041 caggattttc ttcaggggca aatgtatgtg cagcaattaa gttgttaaag agcagtgaaa
     2101 aaggaaaaaa tatagtttta actcttaatg atagtggttt aaaatattta agtactgatt
     2161 tatatgaaga attgtaataa tagcacaata gttatgcaat gttttttaaa ttaagtatat
     2221 agatttaaat tataaaaagt atctcaatgt gatttttgaa agttataaag gagatacttt
     2281 tttattttgt aaatatggag tatcctactt ataagtatta agctatatag atattgatga
     2341 ttagctatgc aataaaaaag agtatttgtt ttgcatttat acatcataac ttggaataat
     2401 acatattaat gttctaaatg tatttaaaac atatgataaa attagaacaa acagatacaa
     2461 ggagataaac atgcaaaaga gaattttaag aaattttata tttacgggta aaaatatttt
     2521 aagtaaactt gtttttataa taaaatataa
//
LOCUS       DAGSHT010000309         2550 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.315, whole genome shotgun sequence.
ACCESSION   DAGSHT010000309 DAGSHT010000000
VERSION     DAGSHT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.315"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..485)
                     /locus_tag="MJB72_003554"
     CDS             complement(<1..485)
                     /locus_tag="MJB72_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBY8264612.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     I"
     gene            complement(647..1864)
                     /locus_tag="MJB72_003555"
     CDS             complement(647..1864)
                     /locus_tag="MJB72_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBY8264613.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFKNVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(2055..2468)
                     /locus_tag="MJB72_003556"
     CDS             complement(2055..2468)
                     /locus_tag="MJB72_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264614.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT          885 a          394 c          294 g          977 t
ORIGIN      
        1 tgaataagat tgtttaattt tatttgccat tcatctttaa atataaattc atgtccatta
       61 tgtattatac cattctggta aattgtagac tcaaaagttg aactagtcga aaaaagagtt
      121 actttttgtg agatagtagg taatttttta actgtttctt ctacaatatt taatattggt
      181 atattaatag actcttttaa ttctttaaag tatatatgtg ctgaattgca tggcattgca
      241 ataaaattta caccagttga ttcaagcttt tgtaatccat caattatagt ttctttcata
      301 agttcatgat ttattggacg gtcaatataa aatggggtag gtaatgaata tatcatcatc
      361 tttggaaatt catcatcata ttttgctcca tattgagatt gacactcatc aataattaaa
      421 tcaacaaaag gggctgttga ccttggaccc attccagcca aaataccaat taatttttca
      481 ttcataaaaa ttatctcctt tatatataaa tatatttatg tataaaataa aaatctataa
      541 aagcataaaa ttatatttta taatagatta tattgtacac ttatataata taaatataaa
      601 gtgatttttt agattatata ttttaatata ctttcttata taagaattat tttaaatcaa
      661 gattatctag tatttttaaa aatattttta cagcagtatc caagtcatct acagaaacac
      721 tttcattagg attatgactt acgcccccag cgcatcttat aaataccata ccaatatcag
      781 ttatattatc catttcctga gcatcatggc cagcaccact ataaatataa aaaggattaa
      841 gatttaaatc tataaaactt ttttctatga tttttgttat tttattagaa catggtacag
      901 gaacattttt aaaagcaagt tcacttgtat aattaagttt tctattttca catatatgtg
      961 atatttcatt aaatatcttt tctacagaat tatcaagaat ttcttgtgaa caagagcgta
     1021 catcaagagt aaattcagct ctttcaggta ccacatttga tgaagatgga tagaaattca
     1081 tctttcctac agtagcaacc aaatcagtag tcgttttagc tactttttcg atagctaata
     1141 cacattcaca ggcacaacat ccagcatcaa gtctcatatt cattggaagt gtaccagcat
     1201 gtccagactt accatttact gatatcttaa aacttttaaa tcctgtaata cttgatacaa
     1261 ttccagcagg aagagattca ttttctaaaa caggaccctg ctcgatatga aattctaaat
     1321 atgcatctac atcttttcta ggatgcaaat ttgttaagtt ttttgtatta aatccaaact
     1381 ttgttacagc ttcttcaagt gtaattccat tttcatcaac agatttaaga agttcctctt
     1441 taaaagtacc agcaaaaact ttacttccca ttaaagaagt ttcaaatcta gttccttcct
     1501 cttcagcgaa agcaattagc ttaatattga aaggataaga cctattattg tgttttgctt
     1561 cttcaagacc aactagaggc actattatac ctaacatacc atcaaatata cctccacact
     1621 ttacactatc ttgatgagat gcaactacta atgtgggagc atttggaaag cttgatttat
     1681 atgtacctat aacgtttcca atgtcatcaa tttcaatatc aagatttaaa ccttccatcc
     1741 atgaactaat taaatcaata gattttttat gttcttgagt caagataagt cttgtaagac
     1801 catttgttgt tgaacttatt tttcctaatg tttgtagata atccatacat ttttttccta
     1861 acatatttac ctcctaaaaa tataattttt atttaagcta agattagctt ttatacttat
     1921 aatttttgaa ctttcaaata attttgtatg tatagtaaaa tactatttta gtattaataa
     1981 tgatataaaa acataaagtc tgtagacgaa gagcagaagt tttaagttaa aaatatgaaa
     2041 tatgattttt attattatag tatttttcta aaataatatc tctttccagt tagaggataa
     2101 ttttcaagtg aaaatacttt atggtatcca aatttctcat aaaatttagg tgcttgaaaa
     2161 ctgaatgtat ctaaaaaaac atatttacaa cctcgttctt tagcaatttt ttcagcctta
     2221 tataaaagtt gacttccagt acctttatat ctaagcttct cactaaccca taagtatttt
     2281 atagaaagcc aattaccatg agtaacacct ataaaacctg caacaatttt acctgcttta
     2341 tctcgaatat atattcctaa atccactggg tttttatctt caagtctagc aagattatat
     2401 tttaataatc cttgaaaaat agtatcttca tcttgttttt ctattttttc tgtaatttct
     2461 aattccatat ttgaactttc tccttgtata atcttttgta tattggtatt ttttaataaa
     2521 atcagtagaa agaatatagt ttaacattat
//
LOCUS       DAGSHT010000310         2531 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.316, whole genome shotgun sequence.
ACCESSION   DAGSHT010000310 DAGSHT010000000
VERSION     DAGSHT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.316"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(89..1519)
                     /locus_tag="MJB72_003557"
     CDS             complement(89..1519)
                     /locus_tag="MJB72_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY8264615.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            1863..2528
                     /locus_tag="MJB72_003558"
     CDS             1863..2528
                     /locus_tag="MJB72_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264616.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT          791 a          385 c          267 g         1088 t
ORIGIN      
        1 aaaaagttca ttttgagata cccattttat ttattacaaa atatcaaatt ttatttattt
       61 gcatatattt tctatatatt ttagcctttt aactttgttt ttgttttaca tactgtagtg
      121 cttttctatt ctgttttact gcttcaagac aaactttttc tgtttgatta tcaacaaatt
      181 ctaaagctct ataattttgc tttactgctt ctaaacatat tttttctgtt tgattattta
      241 catatttcaa cattctataa tcttgtttta ctgcttttag acaaacttct tctgtttgtt
      301 ctttcacata tttaagcgct tcataacttt gattaactgc ttctaaacaa atcttttctg
      361 tttgttcctt tacataagct aaagcattat agttttctct tactgcctct aaacatatta
      421 attctgtctg attttctaca tatttaagaa tttttccatt ttgctttact gcttctacaa
      481 atattctttc tgttggtatc tttacataac ttaatatttc ttttatttca agttcttcaa
      541 atgaacctct tactgcttct atacatatat tttcagtctg tttttttaca tattgcagta
      601 cttttccatt ttgtctaact gactccaagc aaatttcttc tgtttgctct tttacgtact
      661 gtaatgctct tccatcaatt cttactgctt ccaaacaaat ctcttctgtt tgcttattta
      721 catactgtag agccatataa cttcttttta ctgcttccat gcatattttt tctgtctgtt
      781 catttacata atacaatgca aagtcattct gttttactgc ttctatacat atttcttcag
      841 tttgctcttt tacacaagat agtgcactgt agttttcttt tacagcttct aaacatatac
      901 tattactttg ttcctttaca taagctaaag attttccatc tttttttaca gcttctaaac
      961 atatggctgg agtttgctct tttattcctt ttaatatctc tgcaacatca tttttagatg
     1021 ccctttctat aaatgctaga catacatctt ctgtttgctc ttccacaaat ttaaatactt
     1081 ccatgacatc ataagttgat gcatttttta tagcctcaat tagtacattt tctgtcttat
     1141 tatttatgta gtacaaagct ctgtagttct gttttactgc ttctatacaa atatcctcat
     1201 tttgatttgt tatatactta agagatttat atgtctgttt tactgcttcc atacatactt
     1261 tataagtctt gtcctttaca aactcaatta tagtaccatc ctgctttact gctagcagac
     1321 acatctcttc tgtttggttt tctatgtctt ttaactctct gtaatcatat tttactctgc
     1381 ttaaatcatt taaatgtact ttgttatctt cctttaaatc ttcttcatta ttttctatat
     1441 catactcaaa ttcttcatca tctaaatagt catttttttc tggtaaaaat atagatttaa
     1501 ttttattctt tatatccata aaattatcct cctttaatat gattctatca ttaaagtaaa
     1561 tcgtttttaa gcacttattt aattaatttt atattaagtt tatttaaaag tctattcatt
     1621 gtcgatagat tatattttat attaataatt ttatcttata tatgtggggt aataaagtta
     1681 atagtggaca ttttccatcc cattagtata cactatttat atttattact catttatagt
     1741 gatttttttc aaattttttc ttatttacta tatcatattt attcaattta tgatattatt
     1801 taaatataat ataagtttta taaagtatat taaatatatt ataagatgga ggtcgagtaa
     1861 ttttgaaagg aaacatagta caatataatt ttgcagatat agaggaagaa gtatattcgt
     1921 tggattatgc tatagcatgg aatacagacg aggaaaacgt aaacataata ccatttacaa
     1981 ataaattctg taaagaatct atagaatctt tttgcctagg taaaattaat aactttgttg
     2041 aaatattaaa tgaaggtttt gttgaaaatc atcattatgt acatttagat aaaatgatta
     2101 gtgttcctaa gaaaaaggtt aatttagtat atcaacaaga cacacatggt tatcttctaa
     2161 gagatgataa tgacaacctt attcctgcaa aaataacttc agagcaatct aaatctatat
     2221 caagtaagat ggagttattc tgtgctggtg aagaaaaatg tttaattaat atattactaa
     2281 aggctgaccc atcatatatt ttagatgtag atagtataaa agacaaaaac attctaaatc
     2341 taggttatga gtctattgac agatataaag aatacaattt tgatgatgat aagattttaa
     2401 tattttttat aaataaaaaa cgttattcag tgattatgaa aaaaacaaac aactctgata
     2461 acgatttagt ttccagaaac aatgctatta aagaattatt tactaataaa gcaggaaatc
     2521 ttaactaata t
//
LOCUS       DAGSHT010000311         2530 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.317, whole genome shotgun sequence.
ACCESSION   DAGSHT010000311 DAGSHT010000000
VERSION     DAGSHT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.317"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..247)
                     /locus_tag="MJB72_003559"
     CDS             complement(<1..247)
                     /locus_tag="MJB72_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8264617.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKIS"
     gene            complement(278..2512)
                     /locus_tag="MJB72_003560"
     CDS             complement(278..2512)
                     /locus_tag="MJB72_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264618.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSVYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT          871 a          363 c          299 g          997 t
ORIGIN      
        1 ttgaaatttt atcataagga taattttttt cccaatctaa ttgaatattg tgagaatccg
       61 taaaaaattt agcaatatta tcagggagtt tcttagctat ttctatatta gtcattccaa
      121 gtggattatc ttgcatataa cttttaatta aattttttaa attatcattt ataaatataa
      181 ttctgtagtt agaatctctt ataattagaa atcctggaaa agttcttaaa aaaatttctt
      241 taaacatatt aatttctcca aaataaaata ctttgtacta gatttttgtt aaatttttta
      301 ttttatttac atctacatac ataccctggg caatcgacct tccatcaatt gcagaaaggt
      361 tacattgtgt atacataact gtgacaattt gaccatctcg ttttatgaat ctatataaaa
      421 atggttcaat tttatttgtg tcaatcatgt ccaaactttt tttataaaca aagtctaaat
      481 catctggatg aacaattgct cttaaatttc cgcccatctc accaaaaaac tgttcttttg
      541 tataacctat aatatcaaac atttcttcat tcatataagt aataatcata tcaccaaata
      601 aatcgtagcg aattatacct acaggcaaca ttgaataaaa ttgtgataaa atattacttt
      661 catcaagatg tgtatttgtc attttaggta ttgaatatga ttcaaattca tttatatagc
      721 cagcagtttt aattaaatcc aaatgtaatt ttgctaagag taatttattt tctgaaatta
      781 tacaatttgc actaaagtag aaattgctat atttttgatg ttcagaagct tcatctgata
      841 aaacggcttc tccactaatt aataatgaat ctgattcagt attatgtggg gttatacttt
      901 tgtaaagaat agaaaatttt ttacctttaa tatgtttttc aaaatgctct attactttag
      961 gaaaagatgt aatttcagtt ttgttgtaaa aatcaaacca ttgaatgtct tcagtgacat
     1021 tagctcttag ttttttcata tcttgcataa taaaaaatcc atcaaatatc ttctctatta
     1081 aagattttcc tatatcagaa tattttataa acattttctc tgaattattc tctttagata
     1141 gtaatttttc aaattctttg cactccatgg gttttgcata ataataacct tgggcgatat
     1201 cacaatttat acttttaata aattctacat gttgttctgt ttcaacacct tcacatactg
     1261 taagcatccc aagagctctt gcaatgttta ttatagagta caaaatggtt gtatttttaa
     1321 aatcatttaa attatgagat aaaaatatcc tatcaatttt gattatatca aaaggtagat
     1381 tttgcaacat accaaaagat gaatatacag aaccaaaatc gtccattgat acagaaaaac
     1441 cataagaacg aagttcagct agttgttgaa taattgtatt tgtattttct attaatatat
     1501 tttctgttat ttcaatgcta aaatttgatg tagagacgct gtacttttca gtaatacctt
     1561 ttagcatact tggaaaatta gggttaaaaa aatgaagacg agaaaagtta caacaaattg
     1621 gaacaaaaac atcattgtta tcaatccatc ttcttatatt tttacagttc tcttcaaata
     1681 aatgcaaatc taattccgtt atcagaccaa actgttcaaa taaaggaata aatttatctg
     1741 gagtaagaag tcctttaata gggtgtctcc atcttgcaag tgcctcacat ccaactatag
     1801 tattattttt tatattataa agtggttggt aaaatacttc aatttgttta gtttgtagaa
     1861 catgtgatat ttctgataag aactccttat cattttctaa tttattgtaa aaactattat
     1921 ctttaaaatc aaatagaatg tatctatttt taccttcact tttagccttt tttaaagcac
     1981 tatacacttg gttgaaaagc atcaaagtag tcatgtttgg ttcaccattc cagatagtgc
     2041 tgccgatact aaatgtaaca ttggagtata tttctttttc attaggaagt cttattttta
     2101 attctcgggc gctctctata atagttgcac ccatattatt taactcttta tttgatacat
     2161 ttggaagtac aattaaaaaa ccttctcctt ttaaataagc gactattccc tttgattctg
     2221 gaataaagtg caataggttt tgagttagag taattaataa attttctccg actaggtatc
     2281 catatatatc attaaataat ttaaagttat cgatatttaa taacatgatt cctatatgta
     2341 tatttttcga aatacagtca tcaatatgtt ggtacataaa ttctctgttt ggtattttag
     2401 aaaaaaaatc tatttcattt aaggttttta atttaaaatt tctactatct aattccttag
     2461 ataatacttc taccttttta tttaacattt ttatataatt atgattttgc atggtattcc
     2521 tccataagat
//
LOCUS       DAGSHT010000312         2519 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.318, whole genome shotgun sequence.
ACCESSION   DAGSHT010000312 DAGSHT010000000
VERSION     DAGSHT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.318"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            49..210
                     /locus_tag="MJB72_003561"
     CDS             49..210
                     /locus_tag="MJB72_003561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264619.1"
                     /translation="MDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVESCLHQ
                     VMVLVSKYN"
     gene            436..636
                     /locus_tag="MJB72_003562"
     CDS             436..636
                     /locus_tag="MJB72_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY8264620.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(800..1012)
                     /locus_tag="MJB72_003563"
     CDS             complement(800..1012)
                     /locus_tag="MJB72_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264621.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELELGEYGVLGWTNEGLYLH"
     gene            1255..1437
                     /locus_tag="MJB72_003564"
     CDS             1255..1437
                     /locus_tag="MJB72_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBY8264622.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIMF"
     gene            1508..2353
                     /locus_tag="MJB72_003565"
     CDS             1508..2353
                     /locus_tag="MJB72_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY8264623.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
BASE COUNT         1012 a          268 c          326 g          913 t
ORIGIN      
        1 ttatgaaagt tttttagggg gataataatt tatatatttg gaaataaaat ggataaatgt
       61 acacatgtat tgactgctta tattagcagt tcgtatgatt attgtaattt tttagataca
      121 cagttagatg attttatatt agagtacgga gaaaatgtag tagagtcttg tttacaccaa
      181 gtaatggtat tggtaagtaa gtataattag atagacattg atttctagaa aacccttttt
      241 agttttatct acatttatat taatatatag caatgtaaaa cgtttaaata tatttttaat
      301 attaagaata attaaaatgt taactaaaaa atataattaa gtagacattt ctattaatct
      361 atgatacaat agtatttgtg gtaatacaca attaaatcaa gtatatcttg atataggtta
      421 ataggagtat ttttaatgaa taatggaata gtgaaatggt ttaataatga aaaaggattc
      481 ggttttatat caatggaagg tagagatgat gtgtttgctc atttctcagc tatacaaact
      541 tcaggattta aatcattaga agaaggtcaa caagtaagct ttgatatagt taaaggtgct
      601 agaggtcctc aagcagaaaa tataactata ttataatata tagatttttc gggaaagact
      661 ctttttagag tcttttttta ttgttaatat atgtaagata atcattggtg aagtaaataa
      721 ggtatattga gtaatgttct tgatagttta tatgctaatg aatttcaaaa aaataagcac
      781 tcctataaaa agagtgctct taatgtaaat ataaaccttc attggtccag cctaataccc
      841 cgtattctcc aagttctaac tcttttttat atacattatc actttcagcc atatctaaca
      901 caatcatttc ttctttagaa tcaatttgta aaatatgagg actgtgtgtt gtgacaatta
      961 tttgtgtttt tgggagaatt gtttttaatg taataaaaat tttacatacc acatagttga
     1021 tataaaaccc caaaataaac ttagcatctc caatgttcac acatatacaa tgctcttaaa
     1081 attgcagtga acgcgcaata gtgcaattga taatacttat tatatattct caatgcctta
     1141 tatttcaact gctaaacctc aatttgtcac aaatatcgct cactgcaaaa tttctatatt
     1201 tttattatat cataacatat aattaatatt ttcaattaat ttttacattt aattatgttt
     1261 aaattctata ataatgcaac tttaatgttt ctttctggaa ctaatataaa aactatttca
     1321 gaaagattag gtcatgcaga tataaaaata actatgaata gctactctca gtttttagaa
     1381 gagatggaca gagaagcatt tgaaaatttg agcaaaatac tgtttattat gttttaaaga
     1441 agatgactta ttcaatattt gatgctagaa tataattaat aaaaatttaa ttataggtga
     1501 ttgtattatg aatattatta aaagcataac tttactctta acattattaa ttgtgatatt
     1561 aatgacaggc tgttccccaa gagaaagtgc tagtgaagtt gcaaaaaaat atattgataa
     1621 gatgaactct ggtgactatg aaacagcata tagtttatta agtaaagata gtaaaaaaaa
     1681 aattagtata gaaaatcttg aagaatttca aaatatttta gttagtacaa aaagaataat
     1741 tagttataaa ataggtaaag aaaaaatcat aaaaaaatat aaacatgatg gaaaagaata
     1801 taaaaatgtt gttaaattag aagaaacttt taatataaaa aaccttcttt ataaaaaaga
     1861 tgattctcta aaagtaaata gatattttgt agttgaaaat aacaaataca agctattatt
     1921 gtatgaaagt ttcaaaaaag aatttggtat taatagtatg gaccttgctc aacttaagct
     1981 taatgatttt ggactaaaat actttaaaga agttgacctt atactaaaaa aagctattgc
     2041 tattaatcct actgattcaa agctttatta tgcccaagca tgtaattata tgaattttga
     2101 tggatttcct ggttattcag atacaacata taatgcttta gattcaatta ataaatcttt
     2161 aaagtattta gataaaaatg attctgacta caaaaaaaat gcaagtaata tatataactt
     2221 aaaaggagtt attcttatgt ttcacaaaag atttgatgaa gcacaacaat atttaaataa
     2281 agccttatac tttaacaaag ataatgaaga cccaaaatat aatctaaata caataaaaaa
     2341 acatctaaac tagatattta atttttgaat ttataaaata tttttttatg tataattcag
     2401 cactgaatca taatctctac taaactaatt acaagtttaa attataaaaa cttatttcta
     2461 tataaatatt actctataac aataagtttc ttatttaaat tttaaaatat tatctaaaa
//
LOCUS       DAGSHT010000313         2507 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.319, whole genome shotgun sequence.
ACCESSION   DAGSHT010000313 DAGSHT010000000
VERSION     DAGSHT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.319"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            157..534
                     /locus_tag="MJB72_003566"
     CDS             157..534
                     /locus_tag="MJB72_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8264624.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            527..1549
                     /locus_tag="MJB72_003567"
     CDS             527..1549
                     /locus_tag="MJB72_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY8264625.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKSELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNRVNLDCELCCDESVIYNCSFNDKKEYALALIKSIKLSKECNRGILLTDFNRTNLE
                     KRLESIISKKFKNSVFVVIILCLLSSITYFKFDIFISKPVIYSLPKPSVLNDEYIYNT
                     DIRVER"
     gene            1611..2204
                     /locus_tag="MJB72_003568"
     CDS             1611..2204
                     /locus_tag="MJB72_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBY8264626.1"
                     /translation="MLKKIKKVLSIACCLFILGGSISFADLENNNEINDKSVENPIYE
                     FNIQGYTFTYENAPQRIKDEYNKTCRELNITPSPSTEIFIPIDEISKYTDTVPLFEID
                     EFNVKYFGTRFEVTGAKTYTVGVSTYVGYGHTTSGNPVHLAQLLLKKSGYSIAIDSLF
                     GSSTYSKVRSFQSKYGLSVDGVVGLGTWNKLAQVTNA"
BASE COUNT          992 a          238 c          375 g          902 t
ORIGIN      
        1 ttaaatagat atattttaaa atctaacttt taatattgta aaaaaattag aaatttaaca
       61 aaaagtacaa aaattcacat ttttttcata aagtgtttga ctaaaaaatt cacatgttgt
      121 agaattaagg tagcaggagt tttgagggag gtgtaaataa tgataagaaa aataccacaa
      181 gcagaactaa aagtaatgaa attcatttgg aaagtagatg ttacagtgac atcaaaagat
      241 gttattgaag ctatggaaca aaaatatggc tggaaacaaa ccaccacact gacactttta
      301 tctaggctgg taaaaagagg attcttagat gctcaaaaaa tagacagata tacacattac
      361 acaataagag taaaacacaa agattattta agttttgaga caaaggattt cctaagtaat
      421 atacatgatg actctttgag aagtttaatt tcagcattac atgatgatga aaatataggt
      481 aaagataagc tagattttct agaaagttat tttgataatt taaaggatga ataattatat
      541 gctatatgtt tttgaaaatc ttataaagac aactattgta tgtagtcttg gtatttgtct
      601 attattattt ttgaaaagat atttatttaa gaagtttagt aagagattca attattatat
      661 ttggttgata atagtcttta gaatgttact ttttctattt aattatacaa tagtatatga
      721 agtaaaagaa cccaaagaaa atgcattagg aaataatatt acacaaattg atatttctac
      781 agataataat cttatgctgt atgtagctta tttatggtta tttgtaacta tagtcattgc
      841 agtttataca tttataaagt atacaagatt taaaaatcta gttgttgatg tatcttatga
      901 tattgaagat aatgatataa actgtatcta caaaaacttg ttaaaagaat taaatataaa
      961 aaagaaaatt gaattaagag gttctgatga attgataagt ccagcaggca tgggattatt
     1021 taaatcttac atattcttac cagactatcc ttatagtaag agtgagttaa cttggatttt
     1081 gaaacatgaa ttaatgcatt ttaaaaataa agatatttta ataaagtttt tagttttgtc
     1141 tgttagaata atatattggt ttaatccact tgtttatgta atgagcaata gagtaaatct
     1201 agattgtgaa ttatgttgtg atgaaagtgt tatttataat tgttcattta atgacaaaaa
     1261 agaatatgca ttggcactta ttaaatcaat taaactaagt aaagaatgca atagaggtat
     1321 tctattaaca gattttaata gaaccaattt agaaaagaga cttgaaagta ttattagtaa
     1381 aaagtttaaa aatagtgttt ttgtagttat aattttatgt ttactatctt caataacata
     1441 ttttaaattt gatatattta tttcaaaacc agtgatttat tcattaccga agccaagtgt
     1501 gttaaatgat gagtatattt acaacactga tatccgagtt gaaagatgat gttaaattgt
     1561 attaaaaaat gtaataattt aaataaaaga aaatatttta ggaggatttt atgttaaaaa
     1621 aaattaaaaa agtactatca atagcgtgct gtttattcat attaggaggg agtatatctt
     1681 ttgcagattt agaaaataat aatgaaataa atgataaatc tgttgagaat ccaatttatg
     1741 aatttaatat tcaaggttat acatttacat atgaaaatgc accacaaaga attaaagatg
     1801 aatataacaa aacatgtaga gaattaaata taacaccttc tccatctact gaaatattta
     1861 ttccaataga tgaaatctct aagtatacag atactgttcc tctttttgaa atagatgaat
     1921 tcaatgttaa atattttgga acacgttttg aagttactgg tgcaaaaaca tatacagttg
     1981 gagttagtac atatgtggga tacggacata caactagtgg aaatccagta catttagcac
     2041 agcttttact aaaaaaatca ggatattcta tagcaataga cagtttgttt ggtagttcta
     2101 catatagtaa agttagaagt tttcaaagta aatatggact aagtgttgat ggtgtggttg
     2161 gattaggaac atggaataag ttggcacaag ttactaatgc ttagataatt tattataaag
     2221 ctaaaaagaa acatgggagt ctggagtctg gaaatattca tgcatattct tttaatgaaa
     2281 ttttattttt aaaatgacaa ttaaatattt ataaataaga ctatgtttat aaaaattata
     2341 gtcttattta tatatttttt caaaagttat tactccttta aggaaaatcc atttagacta
     2401 gatacataag ttattaatgt atctttaact cctattctgc aagtaatttt aggtaattct
     2461 ttgaactttt attttctcta attaatatta ctaatgtatt ctttata
//
LOCUS       DAGSHT010000314         2507 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.320, whole genome shotgun sequence.
ACCESSION   DAGSHT010000314 DAGSHT010000000
VERSION     DAGSHT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.320"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            127..1674
                     /locus_tag="MJB72_003569"
     CDS             127..1674
                     /locus_tag="MJB72_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY8264627.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1677..2075
                     /locus_tag="MJB72_003570"
     CDS             1677..2075
                     /locus_tag="MJB72_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBY8264628.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2115..>2507
                     /locus_tag="MJB72_003571"
     CDS             2115..>2507
                     /locus_tag="MJB72_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBY8264629.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKK"
BASE COUNT         1025 a          221 c          412 g          849 t
ORIGIN      
        1 ttagtataat gtaatgtagt tatttatatg tgattttaac ttgcattgat ttgctatcaa
       61 ttgtgcttta taattagtat atataaagtt taactgtaag ataatttaaa aaatagggga
      121 atttatatga ataaaaatag catgacaaga atttacagtt taaataaaat aatttttatt
      181 ataattggaa ttttaggaat agttttactc acaagacata ttgaggtaga tattaaagtt
      241 ttaattttaa ttttattaac cttgtttagt ataaatgtat ttatttcata tcttaaaatt
      301 aagttgtatg aggataaaat taatttagga ttgataaata attcagataa gtttataaat
      361 attactaaag atgagaactt cctaaaacat gttcaaaatt atattatcag taatgaaaaa
      421 gaaaactgtg taatggcttg ttttgattta tgtagactca aacttataaa tgatatatat
      481 ggttatgagc taggggatga agtgttaaat aatattttaa gtaacttaaa aaattatttt
      541 ggaaaagaag ctatttatgg aaagttaaaa agtgatgttt ttgtattaat aataaagctt
      601 gaaaataggg aacagaagat tccttattta gtaaatctta ttaagaataa aattgtgata
      661 ctaagtcaga ttgacagttt taaaatggat ataaatattg aggcttctat tggaatttat
      721 aaatttaaca ataatgaaat tgatataaaa aaagctatag ataatgctga tatggcaaga
      781 cttaaatcaa aaggactaaa gcacatagag tatgtagaat ttgataatgc tatggaagaa
      841 gaaatacaat caattatgaa aatagagaga gatttattct tggctattaa aaataaagag
      901 tttgtaattc actatcaacc taaagttgat tcaagtacag gtaacattat aggttcagag
      961 gcattgataa gatggttgca cccatcatta ggcatggttg gaccaaataa gtttatacct
     1021 atagctgaaa aaaatggtct gataaacaat attggtagat ggctaattca agaagttttt
     1081 attaccatta acaagtggat ttcagaaggt ataaatgtct taccagtctc aataaatctt
     1141 tctagagtag aactatataa aaatgattta gttgattttt ttaaactgat gtttaataca
     1201 tataatattc caaaagaact tatagaatta gaaattacag aaacgacagc actaagagat
     1261 gtaaaattta taagtgaaag gctttatgag attaaatctt taggtatgaa tatatcatta
     1321 gatgattttg gagtaggtaa ttcaaatttt atcaacctta aggggatacc actagatatt
     1381 ataaaaattg ataggtcact tatattggat atagttacaa atacaaaaac aaagtttatg
     1441 gtgaaagcaa ttgttaacct ttcacatgat ttaaacgtaa ctgttatatg tgaaggggta
     1501 gaggatatgc atcaggtcaa agtcttaagt gaactgggat gtaatttcat acaaggttat
     1561 gtatttttta aacctttgga tgagatgaat tataaaaaac ttcttactga cggaagttat
     1621 ataaatctga aagatactct tttagataaa tgcatgacag ttgaggagga gtaaggatga
     1681 tttcagaaga tgtagattat tcaataagaa tcataagagc tatatttaat aatggagaag
     1741 ataaaccaat agatgctaaa atgatattaa aaagtgaaga tataccaata ctaacactat
     1801 acagactgct tagaaagctt tgtgcaaaca atattcttac ttttaacaaa gaagatggca
     1861 agacatacat gttaaatgtg tcattggata caataagtct aagaactatt gtagaagtaa
     1921 ttgatggaaa aatatatata aataaatgta cgagatatgt atctaattgc agtaattgct
     1981 taaaaggttg taaagtaaat cgtgaattgc tagaaataga aaaagaaatt tataaacttt
     2041 tatcgagaaa atctataaga caaatactag aataaaaaat tttcagctat gataataatt
     2101 ggaggttttc ggttatggat gatatatctc aagataattt tctactgtct aaagagtatg
     2161 agaatagctt agatgtagat actaaaaaag caagtggaat atattatact cctaaaataa
     2221 ttgtagatta tattgtaaaa aaaactttaa aaaatcacga tataattaaa aatccatatc
     2281 cacgaatact tgatatttcc tgtgggtgtg gaaattttct tttagaagta tatgatattt
     2341 tatatgactt atttgaggaa aatatatatg aattgaaaaa gaagtatgat gaaaattatt
     2401 ggactgttga taacatacac cgtcatatat taaactattg tatatatggt gctgatattg
     2461 atgaaaaagc tataagtata ttaaaagata gcttaacaaa taaaaag
//
LOCUS       DAGSHT010000315         2487 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.321, whole genome shotgun sequence.
ACCESSION   DAGSHT010000315 DAGSHT010000000
VERSION     DAGSHT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.321"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(2..190)
                     /locus_tag="MJB72_003572"
     CDS             complement(2..190)
                     /locus_tag="MJB72_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264630.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            525..1493
                     /locus_tag="MJB72_003573"
     CDS             525..1493
                     /locus_tag="MJB72_003573"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBY8264631.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1610..>2487)
                     /locus_tag="MJB72_003574"
     CDS             complement(1610..>2487)
                     /locus_tag="MJB72_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY8264632.1"
                     /translation="LPRVEEISYREKIIIIHKIYSFMENYIKFPKENLPNRKVMSDIN
                     INDIESLAYKTREFWDLNSTPIVNMVSLLESKGIIISGMNVDRKGATIFTQKQRISKE
                     SKYLISLGNDKKSASIRNYTLACELGYIISSELRIPSKQFSEDEYACAFLLPKESFLK
                     DLTHPEDLDYYVELKKKWIVPISAMIFRAYNLEKINYKKYNYLMNEMDKKGWLIEEPL
                     DKMKGSSPTYLKRAVELLIENKIMSVNSIVSSLEEFGINLYPEDLELLMGLKKGLLSQ
                     EVNKKSKVIKFDGKK"
BASE COUNT          861 a          372 c          305 g          949 t
ORIGIN      
        1 ttcaatctct tttactaatt tcatccatta ttaatgattt tgaaatatac attacttcta
       61 taatattctt aaaacgtgta tgatgagatg tctcctcaga aaatttcttc tgtccttttc
      121 cacaattaat aatacttgaa gatactactc tcagtgcttc caccaattct tcttttgtat
      181 gataattcat aacacattta ctcctttatt ttttaattta gagaaacagt atatctcaat
      241 ttaatatcat tataaactga catcattatt atttatacac atcttttagc acttaaatat
      301 tctaactatc agtaacatta catattttaa taattattaa tatacatata atactttaat
      361 attcactaat agagagtata ttaacattgc ttttttaaaa attttagtat aaaatcatat
      421 taaataaatt ttctgaaata atagaatatt aaaatatcca aaaacagctt ttaataacat
      481 ctataatttt caatgtatcg ttattttagg gggagtgatt taaaatggca ataaagccaa
      541 gaaaaatatc tattgtaggt tcagggcatg ttggttctca ttgtggattt agtttaataa
      601 ctcaaggagt ttgtgatgaa ctctttatga ttgatataga tgaatctaaa tcaaaagctc
      661 aagctttaga tttagctgat gcagtttcat atctgccaca taaagtacat atagaaaaag
      721 gaactttttc tgactgtaaa gattctgaca tagtagtgat aagtgttgca gactcttcag
      781 aagggccttt acgtagacaa aacacaacta gattagattt actaagacca actattggaa
      841 tgataaaatc tatagtaaaa cccatagttg attctggctt tgatggtata tttgtagtca
      901 tatctaatcc tgttgatgta gttacaaatt atatttggga aaaatctggc tttcctaaaa
      961 acaaagtcat tggaactgga acagctttag attcaacaag acttagaagg attttatctg
     1021 aagaaactgg aatcgctcaa caatcaattc aagcttattc tatgggtgaa catggagatt
     1081 ctcaaatggt tccttggtct catgtttcaa ttggtggtaa accaatctta gacatgataa
     1141 aagacaaccc aagtacttat agcaatttag acttaccatc aatagttgaa aaaaataaaa
     1201 aaacagggat aagtataata aatggtaaag attgtacaga atttggtata ggtactgctt
     1261 tagtagaaat tgtaaaagct atactacaca atgagaaaaa agttcttcca gtttctacat
     1321 tactagaagg tcaatataac gaaagaaatg tatttgcagg tgttccatgt gttataggaa
     1381 aagatggtat tgaagaaatt attgaaatca atatgactga atatgagcaa aatgaattta
     1441 ataaatcttg ttctgtttta agggaatata ttgcactttc aaaaacactt tagattcaat
     1501 attctttctt acactaaaag caggtaaatg acttattgaa aagaccttta actaagttaa
     1561 tgtttaaatc atttgtacct gcttttaatt ttaatactaa aatataaacc tattttttac
     1621 catcaaattt tataacttta ctttttttat ttacctcttg agataacaat ccttttttca
     1681 atcccatcaa taattctaag tcttctggat aaagatttat tccaaattcc tctaaacttg
     1741 atactatact atttacactc attattttat tttctatcaa taattcaact gctcttttta
     1801 aatacgttgg gcttgaacct ttcattttgt ctagtggttc ttctatcaac cagccttttt
     1861 tatccatttc attcataaga taattgtatt ttttgtaatt tatcttttct agattatatg
     1921 ctctaaaaat catagcagat ataggtacaa tccatttttt ctttagttct acataataat
     1981 ctaaatcttc tggatgagtc aaatctttta aaaaactctc ttttggcaat aaaaatgcac
     2041 aggcatactc atcttctgaa aattgcttac ttggtattct taattcactt gatattatat
     2101 atccaagctc acatgccaaa gtataatttc taattgatgc tgactttttg tcattaccca
     2161 gagatattag atatttagat tctttactaa ttctctgttt ttgagtaaag atagttgccc
     2221 cttttctatc tacattcata cctgatatta taatcccttt agactctaac aaacttacca
     2281 tatttactat tggagtacta tttaaatccc aaaactctct agtcttatat gcaagagatt
     2341 ctatatcatt tatattaata tctgacatta cttttctatt tggcaagttt tcttttggaa
     2401 acttaatata attctccata aatgaatata ttttatgtat tataattatt ttttctctat
     2461 aagaaatctc ttctactcta ggtagtt
//
LOCUS       DAGSHT010000316         2483 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.322, whole genome shotgun sequence.
ACCESSION   DAGSHT010000316 DAGSHT010000000
VERSION     DAGSHT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.322"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            341..820
                     /locus_tag="MJB72_003575"
     CDS             341..820
                     /locus_tag="MJB72_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8264633.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            817..1596
                     /locus_tag="MJB72_003576"
     CDS             817..1596
                     /locus_tag="MJB72_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264634.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDEVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1846..2346)
                     /locus_tag="MJB72_003577"
     CDS             complement(1846..2346)
                     /locus_tag="MJB72_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264635.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT          986 a          335 c          308 g          854 t
ORIGIN      
        1 tttaaatctg tacatgaagt aaataatcca aaagcattgt tattctaact actcaacatt
       61 aaatacttac tattttctaa ttactcaata taaacactta ctataaaact aaatatataa
      121 atgacttcta aagtttaata tatctacatg gctttatata tgatgggaaa atactagaaa
      181 aaaatatatt atagaatatt tgtatagaat aggcaaaact ttcaaactta aatttatcaa
      241 ataatcataa aaaaagtgaa ccatctatag taaaatttta tctaataagt gaaaggcaaa
      301 aaagtagcgc tacgatttgc atagaaggga aggaacttag ttggataaaa ctacatttac
      361 aaacaatata cttgaatcag aacagacttt gtatcgagta tctaagtcta ttctaggaaa
      421 cgaccaagac tgtgaagatg ccgtcaacaa tgctatttta aaagcatatg aaaagttgga
      481 ttctcttaag gaagaacaat actttaaaac atggctgatt agaatagtca taaatgaatg
      541 taattcatta agacgcaaac gattaaaatc tctatcattt gaagatgtct tcaaaaacaa
      601 aaaaattgat gaaaaagatg actatagcga tttgtatact gctattcaaa gtctacctaa
      661 aaaaattaaa attccaatag tcctttacta tattgaaggt tattcagtag atgaagttaa
      721 ggaaatatta gatatacctc aaggaactgt aaaaagtaga ttatcaagag gaagaagatt
      781 attaaaaact aaattagaaa acacggaggt aatcatatga ataaaaatat attaaataat
      841 tcgtttccag aagttccaga aagttttcat aatagtttaa gcaaaacact aaatagttta
      901 ccagaaagag aggaaaatta taaaatgatt aataataaaa cacacaaatt accatttaga
      961 aaaggcttag ttgcaacttt agccattact ttgatattga gcacaacagc ccttgcagta
     1021 ggacatatat atagtttggt tggaaaatca agtactaagg ctacctatac atctatccca
     1081 actacagaac aagtaaaaaa agactttaac tttaccccta atatagtttc tgaatttagt
     1141 aatggatata aatttaaagg agcatatact gtaaataaaa aagcatttga caaagaaaat
     1201 aatgttttag gaaaatcaaa agcacttagt tttgaatata aaaatggcaa tgatgaaata
     1261 tctcttgaca cagcaaataa tgtacttggg aagagttctg acaatctaaa agttgtagat
     1321 acttacaaag atattaatat ttcttatgaa gcattcgcac aaaagtttgt accagaaggt
     1381 tataaaatga cagaacagga taaaattgat gaaaaatcag gcaaatacac atttagtgta
     1441 gggccaaata atgaatcaga caaagttgaa gttaataaat ttaagttttt gacatggaaa
     1501 caagatgaag tttactattc ttttacagtt caagactcaa atttatctat gaatgactta
     1561 gttaaaatgg ctcatgaagt aatagatgca aaataaacat aagtaataat aagttgttta
     1621 tgagtttatt atttatgact ttaatttttt tattagtaaa catggttgta tcttccatct
     1681 ttatattttc tttaaattta atatataaaa ttttagatat ttagctacct tcaaaagaac
     1741 cattattaaa aagcccttaa aaatatttct tttatatgga attttaaggg ctttaacttt
     1801 attttataca agtgtatatg gaattttaaa gactttaact ttattttata taagtgtaag
     1861 ctccatttga attacttctt cttttacagg tctatcattc catgtaaaac cactagatat
     1921 tgtatttacc tctacaaacc ctaaagattt ataaacatga atagctctac tattactttt
     1981 aaatacatca agctcaattt tttcataccc atattctttt gataaatcaa taatttgttt
     2041 taaaagatta cttccgatac ctttttccct atactcttta agaataccca tgcctaaata
     2101 accaactttt tcagttttag gtaaaacgtc acaccaacca atacagttat ctgattctaa
     2161 atcaattata aacagttgtg gtaagttttt atttataata ttttttataa attcaactgt
     2221 ttcttcaaaa ggaaaggctt catccatagc tagatatttc ctctcttttg ctattttatc
     2281 aaaagtttgc caatacgaat aaatatactt ttcttctgcc tttataaatt ttatgccttc
     2341 tatcaactta acatttcccc tttttaattt gtgattgtag ttaacttatt ttatatgatt
     2401 ctagtttata atgctatata aattactata tattagctta aaatatcttt gtatatttct
     2461 ataaaattaa aaccaatata tct
//
LOCUS       DAGSHT010000317         2481 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.323, whole genome shotgun sequence.
ACCESSION   DAGSHT010000317 DAGSHT010000000
VERSION     DAGSHT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.323"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            373..1674
                     /locus_tag="MJB72_003578"
     CDS             373..1674
                     /locus_tag="MJB72_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8264636.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1750..2025
                     /locus_tag="MJB72_003579"
     CDS             1750..2025
                     /locus_tag="MJB72_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY8264637.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
BASE COUNT         1026 a          199 c          478 g          778 t
ORIGIN      
        1 acgatatatt aataattggc agtaaattat attaaataag atattaatat cgagtaatga
       61 attatattga acgatatatc aataattggc agtaagttat atcaaataag atattactat
      121 cgagtaatga attatattga acaatatatt aataattggc agtaagttat atcaaataag
      181 atattgctat tatgtagtaa attatataaa acaatgtatc agaataaata gtatattaat
      241 aatttgtagt atatcatgac gaataataca gtcataacaa atagtgagtc ataacaaata
      301 atgtatttat aattgataat gtatttaaaa atcaaaatat aaaaacttat aaggaaagag
      361 agattaattt atatgggtaa atcaaaagat gttgaaatag agaagtctat tttaggcaca
      421 attcttttag acaataaatt aagctataag ctagatgaat taaatgaaaa catgtttatg
      481 aatgatatat gtcttgagat atttaaaatt atgaaagaac ttaaaaaaga aaacatagtg
      541 attgatgtgg ccactgtgaa atcaaagata gatagaaaat ctctagcaat aaaaacgagt
      601 gatgtgacta atctaattac ttggggtcag aattttggtc ttgatggtca tattaaaatt
      661 ttaaaagaaa atttagctag aagaagcata aatcaaaact gccaaaacct tttacacagc
      721 ctaaaccttg gagaaaatat cgatacttgt atttacaaat ttgaaagtaa tataaaggag
      781 attttggaca aggatactta tgaaaatgat gatgtaaata gtatagcagg gaaggtgctt
      841 gattttttag aaaacaaaaa agatattgga tttaaatttg gtataaaatt acttgatacc
      901 actataggag gtctttttaa aggagagcta accacaatag cagctaaatc gggggttggg
      961 aagactgctt tggcacttca aataatgtta aattcattta agcaaggtaa aaaaacttta
     1021 tttataagta gagaaatgac cagtgagcaa gtatttatga gaaatatatg cagagtaact
     1081 ggtgtaagta caaggaatat gaaatctaaa gaaatagatg aaaatgattg gaaacttatt
     1141 gtaaatgcta taggtgattt aagcgaaaat aacttaattt atatcaatga taagatagat
     1201 acaattagtg caattagaaa gaggataagg caggtaaagc cagatttatt aatagtggat
     1261 tatgtgcaac tccttacatc tcagaaaagc atggataaga gagaaaggga agtagctact
     1321 ttttcgagag aacttaaaaa tatgacttta gattttaata tacctgtaat ccaattaagt
     1381 cagttaaatg atgaaatgaa agattcaaga ccttatggag atagacctat gagggatagc
     1441 aaagccatct accacgatag caacaatgtg gtttatatac atcaattaaa ggggagtgat
     1501 tatgaagaag cagtgagaga tattggagaa agtgaggagg cagttagagc atcagaatac
     1561 aggggaatta agatggttga tttaatagtt gcgaagtgta gagatggaca gactaggcat
     1621 aagcattttt gttactttgg ggataagtta cattttcaag aactaaatta ttaggtcaat
     1681 aaagtagtaa taacaagctt attaaaagtc gggaaatagc aaatagatta taaaatatga
     1741 ggtgaaaata tgttggaatt aagaaaaatt atagaattac ttgaagaaca caagatagaa
     1801 gtaagtattc caaaggagtt acataagtat agtttattgg aataccttga attgtgtcaa
     1861 caaagtgggg gaacatattt ttatattaaa acactggaag gggctttaaa agaggctaga
     1921 aatgagctta tgattaaatt gtttaatggg aaaaattata tggaattagc gaggatgttt
     1981 gaaatgagtg ttattaatgt caggcatatt gttagaaaaa aatagatatt tttggagaat
     2041 acaagtgtga tatacttctg aaatagaagt tattatagtt gaaataaaat tgtggttaat
     2101 aaattaaagt tgtgtataag tttaagatta tagaggagta taagtgtgat taacaacatg
     2161 attttgtgaa taaaattgag gatatatatg agttaaatgt gtgattaata aggtaaggtt
     2221 gtgtataaga ttatgagtat ttaaaaggag gggctataat taatagtgta gagttgtgtg
     2281 tttgcttaag attatataat aatttaatag attaaactat actaataagg taaaatcgtg
     2341 tgtttagtta aagcttatta taaaatcttg atatgtgatt aatgatgcaa tatgtgaata
     2401 aagttaagat tataatagat ttaacctgtg gctaatagaa taaaattgtg aataaagtta
     2461 agattataat aggtttaacc t
//
LOCUS       DAGSHT010000318         2474 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.324, whole genome shotgun sequence.
ACCESSION   DAGSHT010000318 DAGSHT010000000
VERSION     DAGSHT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.324"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(127..924)
                     /gene="cas5"
                     /locus_tag="MJB72_003580"
     CDS             complement(127..924)
                     /gene="cas5"
                     /locus_tag="MJB72_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBY8264638.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            complement(940..1953)
                     /locus_tag="MJB72_003581"
     CDS             complement(940..1953)
                     /locus_tag="MJB72_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBY8264639.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            complement(1973..>2474)
                     /locus_tag="MJB72_003582"
     CDS             complement(1973..>2474)
                     /locus_tag="MJB72_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264640.1"
                     /translation="DNYNFSREPGNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTG
                     TSREMDFVSSSYNIGLYIKLCWIVDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESR
                     RKNFSMKVIQLLKSNDRTQLFQTIMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLF
                     IQEIMK"
BASE COUNT          740 a          446 c          232 g         1056 t
ORIGIN      
        1 caaataaatc cttaatatgt tcaactaatg tttgttcttg ttgattagtt cttaaattgt
       61 atgacttagc taatatttct ttttgcatga tatttttcat gataaacacc tatctttcaa
      121 aataatttaa agccatgtaa aaacttcttt tgtatcaata catacattta ccttaatatt
      181 tttaggatat ataccatcat tatcaacata tatataatgg caaaactcat aaactctatc
      241 atctctattt gcttctaaat ctttatattt atgaggcaat gcaatcaata caccttgatt
      301 tattatagtc tcgacattct ctttatctct taagtcaact cttacatatg tattctttaa
      361 ttttatattt tcttttatag catcacgagt attcataggt ataacctctt ttaacttaac
      421 ttctttcaca aaacaccctt tttcacttgg tattacaaaa tcttcttttc ttcctaaatt
      481 tgcatattta catggacttt caagcttttt tctaataaat tcaagttctt catcatcctt
      541 gcaagaaata aatatagtca gttctacttc tttaagaact tcatagttta atatgctatt
      601 ttgagcttta tgctctttat agtaatcata gtagtttcct ttaagcaaaa acttttcttt
      661 atctttacct tttcccattt ttatatattc attccaaaaa tcaatgtttt ctcctcttga
      721 aaattctagt tctctatgat gatgtgttcc aattattcct atatctatat cagagtttaa
      781 tcttctatca cataaatacg ttatcattcc cattattgtg gtcttagatg gtaaatttaa
      841 agtacttata tattcatttt gaaatacttt tgggtctcta tagtgagcag ttggttgctt
      901 aagtttacat tgtaatactt tcataattca aaaaacctcc tactctttta aataccacat
      961 attatcttct ccaatttcca atctatctgc tagattttta atctctttta cagggtattt
     1021 acttaaatta aacttattta attccttttt attagaaatt tttaatctat cattgatagc
     1081 aagtattgtg ttactttctt taaggctata actttctata gtttctatga ctccctctaa
     1141 atttatctca ccactatcag cattataatc tacatcatcc caaaaaaatg gtacaacttt
     1201 atcaaaagct ccacctatta ctgcaagtgg ttttaatagt actgatgaat gttttatgtt
     1261 tcttgtaaac acagtaattg catctaatat atcttttact gctttttctc ttaactcctt
     1321 actcataaat tttctttctt ctggtaaaag taactttcca tcttctactt ctactactcc
     1381 tattctatca agctccaatg ttattgtata aacgtagtaa tctgataaag tctcttctat
     1441 tgctaatgct tgtgatgttg gaaagttttc atctctaggt acttcatttt cttccttctc
     1501 atcataatat tttctaccaa attcactact taaaaagcct ctatttgtta ttagctgtgt
     1561 tgaaccttta tatggattta atgacctaaa aggtattatt ctaagtggag aagctttact
     1621 tatagaacct ttatctttaa gtggtattaa ataaccaaat acatcagtct ctaatccatt
     1681 ttcatctaca catcctacat ttttcttaat agatggatat atatttcctt tttttccaaa
     1741 aacaatatta cttaaattcc aaccatattc ttggtggagc attcttctta aatcatatgt
     1801 tattgtggct atactagttt ttgcatgtat tttttctcta tattgttctt tttttattgg
     1861 tgtataattt ccataccctt ggtcatatga aagtggaact gggctttctg tcagtatagt
     1921 taatgttact ccttttactc ttttttcatt cattttaaat tttccccctt ttttatttca
     1981 tgatttcttg aataaataga cctacattcg tatttaattc taattcagaa ctctgcatta
     2041 gaccttctac aaacccttct cctattaaga ttccattaga aactaacaca tgcattatag
     2101 tttgaaatag ctgagttcta tcattagact taagtagttg aatcactttc atagaaaaat
     2161 tttttcttct ggattcatct gtcattttat taaataagtc tttacctact cttgaatata
     2221 ttaaattact ttccatatag tttttcattt tatactcctc ccctccatct acaatccaac
     2281 acaattttat atataaaccg atattatatg agctagatac aaaatccatt tctcgagaag
     2341 ttccagttga ttgctttaaa acacttatat tatcaagtaa taattctttt aaatttgata
     2401 aattcccatt atcaatcatt cttttaccca aatttctagt attacctggc tctcttgaaa
     2461 aattataatt atct
//
LOCUS       DAGSHT010000319         2467 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.325, whole genome shotgun sequence.
ACCESSION   DAGSHT010000319 DAGSHT010000000
VERSION     DAGSHT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.325"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            372..1193
                     /locus_tag="MJB72_003583"
     CDS             372..1193
                     /locus_tag="MJB72_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBY8264641.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(1503..2282)
                     /locus_tag="MJB72_003584"
     CDS             complement(1503..2282)
                     /locus_tag="MJB72_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBY8264642.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          899 a          358 c          296 g          914 t
ORIGIN      
        1 ttaactttct ttgtacaata atacaatgat aaatataaaa aattatatat tccattctat
       61 agaaatatgc tcaccaataa catgtatgtt gttaatcaat atattatatc aaattatatc
      121 aaaattacat aaaccttata ggacaatcat atcttattac aagaaaaatg tcttatccat
      181 attgaataag tcattcggtt catatacaaa gtcaatttca ttgagaaata tttaatatat
      241 gcttataaaa atttcatact attctttcat atttacatgt gatataattt attaactcat
      301 aattttgtta atcagttgtg attgtagatt atttatataa tctaatattt ttagaaggtg
      361 gtgtaataac tttgaaatta aaaattgttg gtagtggcgg tatgtcaatt attccaagtt
      421 cattttgcaa atgcaaaata tgtgaagaag ccagacaaaa aggcggaaga tatgaacgat
      481 taggaccaag tttatatatt gatgatataa aaatgctgat agacacacct gaagacattg
      541 cagtagcttg taacagacaa aaaatatcgg aagtaaaaca tatttcaatt tctcaccatg
      601 acccagacca tgttaagggt attagaattg tcgaaaaaat aggatgtgac tttatcacag
      661 gagaaaataa tccaattggc ttttatgcgc ttcctgaagt aattgaagat ataaaccgaa
      721 cgaatctcga ttgtcttaaa tattatggtg atgttataaa ttgcatttct attaatgaaa
      781 cttcgcatgt aaagataaat aattttgata ttgatttgat taacaataac ccagacagga
      841 atataacctt ctatgttatc aaagagaatg aaaaaaaagt catatatgcc tgctgtaatc
      901 ccaagccatt tactcatagt gatatgtact ttgatgctga tattttaata ataagtctag
      961 tttctgatga tggtatatta aatgatggta caaaacttaa agacacacca tttaaagatg
     1021 aaatatttac tttagatgag attgttgaaa ttaaaaacta ttatagaatt aaaaaaatta
     1081 ttgttacaca tattgacgaa atgtggggaa aatcatatgg ttattacagt gaattagaaa
     1141 agaaacttga taacatttat tttgcatatg atggtatgga aattatagtc taaatcatta
     1201 agtaataagc ataatgcaaa ataagaaaac ttcaatttaa cagtatagtc aaactatgtg
     1261 atatcaacat atcgcagttt ttacaaaata tcatagtatt tatctttata aaatttcaag
     1321 tcctacatat tttaatactt caatatctaa ataaagtaaa atcaaaaata tattttatat
     1381 acattataga cggcttatcg tcactagata agccgttttg tcacaattat tatttgctca
     1441 acaaaacttc tcataatata taacagattg aatctccaac tgatacttaa ttaccaaaac
     1501 tcttattcct taatatgttg ttgcattaac caaaccttca tcatcatttt ttgtggcaat
     1561 agttttgcta ataaatgact ggattttgta tttataccat acacagaaat atccttattt
     1621 ttctttgcat ccttcaatgc ttttttggca acaacgtctg gagacactat gcctgggaaa
     1681 ttttttgtcc ctttttcagc tacaactata cctctcttaa atagatttgt actcatccaa
     1741 cctggacata cagcaattgc atttatccct ttactcttaa gttcaacatt taatgctctt
     1801 gtataatttc ttataaacgc tttagttgaa ctataaatat tttgatatgg caatggctgg
     1861 aatgatgctt gtgaagaaat attcatgata tggcttccct tttgcatata tggaatacat
     1921 ataagtccca atgctacaac agcactaata tttaaatcaa tcatattaat agattcatct
     1981 atacttaaat cattgtatga acagaatttt gcaaaaccag cattgtttat aaggtatttt
     2041 atacatacat tttctttact taaaaatgta ccaatttctt ttacttcttc tatttttgat
     2101 aaatcttttg aaaaaatttt taccttactt ccaaattcat ctactaattg atttaatctc
     2161 gccttgtttc ttgcaactgc ccatatttct gttatttctt cttcatttat taataacttt
     2221 agaaactctt taccaaaacc actattagca cccgttataa ttgcaatctt cttataactc
     2281 ataattattc tcctttacca taagtttata cgctcaaact gtaaacttat tttttacaaa
     2341 taaaacaccc tctattcaga tgttatattc atattatttg agatattata tttatatcat
     2401 tttcaataat tgttatatag cataaaaaat agtgttaata ttatagaatc ggtattgatt
     2461 atattta
//
LOCUS       DAGSHT010000320         2365 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.326, whole genome shotgun sequence.
ACCESSION   DAGSHT010000320 DAGSHT010000000
VERSION     DAGSHT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.326"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1344
                     /locus_tag="MJB72_003585"
     CDS             <1..1344
                     /locus_tag="MJB72_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264643.1"
                     /translation="SKKQEKIPKVGIGIYILAIVSIIVIGYGYYLSTNIRTGDITSVL
                     PVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNINYRLNKNYKTYAII
                     AIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVNKKQIENIIKDTNKI
                     KYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKELKEYGERLVSGNNV
                     IELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQSIIVVNDDTYFKLR
                     DKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQGESQWMKFAYVVLV
                     FLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINRAILKEVAIFYTLPM
                     LSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVSVNKFKSVVYKN"
     gene            1496..2176
                     /locus_tag="MJB72_003586"
     CDS             1496..2176
                     /locus_tag="MJB72_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264644.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
BASE COUNT          978 a          192 c          407 g          788 t
ORIGIN      
        1 tctaaaaaac aagaaaaaat accaaaggta ggaataggta tatatatatt agctatagta
       61 tcaattatag taataggata tggctactat ctttctacca atataagaac tggagatata
      121 actagtgtac tacctgttat aggtataata atagtaggta cttatggatt attcaaatca
      181 gttatgcctg ttatatttga tattataatc aaaaataaaa aaatattatt taatggaaat
      241 aatataattg caataaacaa tataaactac cgattaaata aaaactataa aacttatgct
      301 ataatagcta taattataac tacaacaata tcaactcttg gtgcttcagt tgcgatgaag
      361 cgtatacata agaatgccca agaacaaaga aatgtgtaca ctattagcat agtttcaatg
      421 gataaagatg aagtgaataa aaaacaaata gagaatataa taaaagatac taataagata
      481 aagtactcag taaatccaga actaacaatt ttaaagaaaa cagatataaa agaagcttat
      541 gatacaacag atactataat gaaatatagt gatttttgta atactcttaa agtgaatggc
      601 aatgataaag aattaaaaga gtatggtgaa agattagtta gtggaaataa tgtaatagag
      661 ttaaaaagcc ctcaaacgat agcatcaata gctacaaaag aagccaagat aaaacttgat
      721 ggtcagttat ataatatttc aaggggagaa gtaaaagttc cagtactagg gacaggactt
      781 aatcaaagta ttatagtggt aaatgatgat acatatttta aactaagaga taagggtcaa
      841 caagtgtatt tttatggtgc aaaggtattt caagaagaaa atagtcaagt aatgttcaac
      901 aatattgaaa agaatttaag tttaaaaaat gtgtatattc aaaatggata taaagctcaa
      961 ggtgagagtc aatggatgaa gtttgcatat gtagtgcttg tgtttttatt cttagtcttt
     1021 gcaattgtag ctggaagtat aatatatatg aaaatatata gtgatgcata tgaagataaa
     1081 gagaaatata agattttgtt aaaaataggt actacagaaa aagatataaa tagagctata
     1141 cttaaagaag tagctatttt ttatactttg ccaatgttaa gtgcaactat atctagttat
     1201 tttgcattga gattagcagg agatttattg atgaccgatt tatttggtat atatatacta
     1261 agtttagtga tatgtttgat aatattcata atatatggag cagtatctgt taataagttt
     1321 aagagtgtag tgtataaaaa ttagttgata tattaataaa gagaaaggag attcttgtaa
     1381 taagagtctc cttgttgatt aactaaataa tttgggtaat aaattcatat aaaatatgtt
     1441 atgatattaa taattcatag catagtgaaa tattaggata atggagaata tacaaatgta
     1501 tagaatttta ttagtagagg acgatattga tttatcaaaa gagattgcat tagcacttga
     1561 aaaatgggga tttaaagtag aattaattga tgattttgag gtagtattag atgaatttat
     1621 agataaaaaa ccagatgtag tacttttaga tgtaaattta ccattatata atggatttta
     1681 ctggtgcgaa aaaataagag ctatttcaaa tgtaccatta atatttttat catcaagaga
     1741 ttctgatatg gatttaataa tgggtataaa taatggagca gatgattaca ttaccaaacc
     1801 attttctata gaaatattgg ttaccaaaat aaatggtata ataagaagag tctataatta
     1861 tagtgattct aatagtattt tgtattgtga agatttgatg tttgatgtgg ggaaagggat
     1921 tataaaacat aagtataagg ataaaagtat agagttgact aaaaacgaaa taaaaatttt
     1981 aacattatta ttaaaaaata agaatagggt tgtatcaagg gaaagtctta tgatgactct
     2041 atgggataat gatgagtttg tgacagacaa tgcacttact gtaaatatga atagattgag
     2101 aagtaaagtt aaagaactag gttttgatga ttttataaaa actaagaagg ggataggata
     2161 tataatacaa tgctagtaaa atatttgaaa ggtaggtcta agtatattgc tctaccttta
     2221 gttgtagtta gtataataaa tatctactta tttgctatag atatttttaa aaataaatat
     2281 tcagagttaa tatatttaga ttttttagtg ctgtttataa taatggtatt ttttgttata
     2341 gattatataa attttagaaa tagtt
//
LOCUS       DAGSHT010000321         2359 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.327, whole genome shotgun sequence.
ACCESSION   DAGSHT010000321 DAGSHT010000000
VERSION     DAGSHT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.327"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..705)
                     /locus_tag="MJB72_003587"
     CDS             complement(<1..705)
                     /locus_tag="MJB72_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY8264645.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITE"
     gene            979..1653
                     /gene="fsa"
                     /locus_tag="MJB72_003588"
     CDS             979..1653
                     /gene="fsa"
                     /locus_tag="MJB72_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBY8264646.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GIKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1775..>2359)
                     /locus_tag="MJB72_003589"
     CDS             complement(1775..>2359)
                     /locus_tag="MJB72_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBY8264647.1"
                     /translation="CLGIMFFMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEI
                     NWKVLIFFIAAFSIGGVLKYSGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICL
                     GSAVTTSSVVIPLLGSLHILKENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYN
                     SKVIFKYGLTLIPLTYFIIICITFPWWKFIGLEI"
BASE COUNT          903 a          306 c          314 g          836 t
ORIGIN      
        1 ttctgtaatt atagaactaa tatcttctaa atgagcaaac ttgaaatttc catcaaattt
       61 aaacttttta tcttccataa ctataaatac ttttttactt gaatttatta tagccttctt
      121 cgtaagacca tcttcaattt caaaagttgt aagtgaagag ttgaatacat ctactccaca
      181 acttcccata aaagtcttat caaacttata tttcgatata aactctatag ttggagtacc
      241 gacaaaaccg tctaaactga gatttaataa acctccagtg gaaattatat ttaaattatt
      301 tggttttgaa gtaactacat ttagtatatc cagcatattt gtaacaagtg ttactttttt
      361 attactttca gctaacaact ttgcaagcaa gatgttaatt gtagagatat ctaaaaatat
      421 agtttctcta tcttcaatta aattaaatgc tttttctgct attattttct ttgttggaat
      481 attaatttct tttctgtctt ttgtatcctg attctctagt gtttctctag ctagtacagc
      541 tcctccataa attcttctta ttgaagaaat cttttctata ttttttaaat cttttcttat
      601 acaatcttct gtaacattaa atttagaact gagctctttg acagtaactc taccttcttt
      661 ttttaggata tttaatatag cttggattct ttcttctgca aacattttct acctcttttc
      721 gcaatatagt attatagtaa atattataag ataaaataca atatatcaac tattttaaca
      781 tgaaatacaa aattaattta atataaaaag tatgtttttt ctaactatat atttagaatt
      841 tagtaaataa aaaaattata aaactaagaa ggaaaagtaa aacaataata aaaaataaaa
      901 aacaataagc aaaatgttga aaataatacc aaatcaatat ataataagaa taaagaaaga
      961 ttaaaggggg atattttaat gcaactaatc ttagatacag gtaatgtgga ggagattaag
     1021 gagttatgta cttgtttacc aatagatggt gtaactacaa atccaagtat tgtttcaaaa
     1081 gaaaaaaaga attttaagca acttataaat gaaattggtg agattatagg tgaggatatg
     1141 cctatacatg ctcaagtgtt aagtacaaag tatgaagaaa tattagaaga agcattatat
     1201 ataagtagtt tgagaaaaaa tatatatgta aaaatacctg taacacaaga tggtttaaga
     1261 gctataaaag accttcacag aaaaggtata aaaataactg ctactgcaat attcactgct
     1321 catcaaggtt ttttagctgc aaaggctgga gcaaattata ttgcacctta tgtaaatagg
     1381 cttgataata tatctggtga tggagtagct atggtttcag aacttataaa aataatagat
     1441 acttataaaa tggatacaaa agtacttgca gccagtttta aaaatgcaca acaagttata
     1501 gaattaatgc aacatggggt gcatagtgtt acagtaccat atgatatttg taaatctatg
     1561 atgaatcatc cattaactga ttggagtgta gataagttta tagaggattg ggagaatact
     1621 tttggaaaag gaagtaaaac aaataatatt tagtattatg gtaaaagagt agttttgtcc
     1681 aatttcacaa gaacttatat aaaaagatga aaatatgaat tttcttttta ataatttcgt
     1741 tattatctta agttaattca tattttcatc ttttttagat ttctagtcct ataaatttcc
     1801 accaagggaa tgttatacat attattataa agtaggttaa aggaatcaaa gttaaaccgt
     1861 atttgaaaat aaccttgcta ttatacagat tttcaccatg accaaccatt attgtagcat
     1921 gatgaaatgg taatatgtac tgtatgctta ctataatgta gacaaataga cataaagtaa
     1981 cactattttc ctttagtata tgtaaacttc caagtaacgg tattactaca gatgatgtag
     2041 tgacagcact tccaagacat atattcaaga ttattgtgag agttatcaaa aacaatattg
     2101 aaataatttc tgaagagttt ggtataaact ttatcaaata atttcccatt atatctacaa
     2161 ctccagagta ctttaacact cctccaattg aaaatgctgc tataaaaaaa ataaggacct
     2221 tccaatttat ttcttttaaa gtactaaatc caactatttt tcttagtaat aaaagaagta
     2281 cacaaattga cattataatt aatgtgttta tgttatgtat gaactgagtt aaaaacataa
     2341 aaaacattat tcctaggca
//
LOCUS       DAGSHT010000322         2355 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.328, whole genome shotgun sequence.
ACCESSION   DAGSHT010000322 DAGSHT010000000
VERSION     DAGSHT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.328"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..558)
                     /locus_tag="MJB72_003590"
     CDS             complement(<1..558)
                     /locus_tag="MJB72_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY8264648.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDV"
     gene            944..1678
                     /locus_tag="MJB72_003591"
     CDS             944..1678
                     /locus_tag="MJB72_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY8264649.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1821..2312)
                     /locus_tag="MJB72_003592"
     CDS             complement(1821..2312)
                     /locus_tag="MJB72_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBY8264650.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          831 a          310 c          328 g          886 t
ORIGIN      
        1 tacatcagtt cttacattaa cattgcctgc ttttagattt tctccaatag taagaccttt
       61 ttcttttaga acatattcaa aagtcattag tggaactcca ccttttctac cacctaatat
      121 ttcggttcct tttaaatcat tgtaagagaa attaggcatt tttttcctag caactagaaa
      181 actaccatct ctttttgtca attgagcaaa gttcactgca taatcgtttt tgttttgatt
      241 atatacatat atagatgctt caggacccat taaacctacg tttgcttcac ctgaaatcaa
      301 agctgccata gttttatctg caccttgggt atttataagg tcaatcttta taccttcatc
      361 ttcaaagaat cctttcgtaa tagcagcata ctgtggagca taaaaaacag agtgagtaac
      421 ttctgctata gttactttat ccagattttt agacgaagaa gattcacctt ttttgtcatt
      481 agagttaaaa tcacatccag ttagacatat tgtacttaca atgatagagc ttgctattgc
      541 gactattttt ttaaacataa taaatacctc ccataagaca tacgaatagt atatagtatt
      601 aaaaatttat tgtagttgtt acaaaaatgt atctatgctt ataatatttc tacaaaaact
      661 taataataat tttcagcttt atgtttagtt ttttcagtaa tgtgtaatat tggataaatt
      721 atattgatta tttataaact aagtaataaa attaaaataa tatcactttt ttgtctatta
      781 tatataagga aaaatttttt aataatataa atcatattca tttatattat taaaaaattt
      841 gtttatagaa acttttatta ttttatataa aagtgataaa aacttatatt ttgtatttaa
      901 tagagtgttg ctaagtgaat atatttaaaa tggaggaaac tttatgaaac aaaataaata
      961 tgatgatgat agatttttta ataaatacag taaaatggaa cgttctacaa atggattagc
     1021 aggtgctgga gagtggcatg ttttaaaaaa aatgttacct gatttcaaag gaaaaagagt
     1081 tttagattta ggatgtggat ttggatggca ttgtcaatat gcagttgaga atggagcaat
     1141 ttctgctgtt ggagttgata tttcagaaaa aatgttaaaa gaagctagaa acaaaacgca
     1201 acttgataat ataaattaca tatgtatgcc tattgaagat attaattttc caaaagatag
     1261 ttttgatgta gttattagtt ccttggcatt tcattatatt caatcatttg aagatatatg
     1321 taagaatata agtaattgtc tttcaaataa aggtaatttt gtattttctg ttgaacatcc
     1381 tatatttact gcatatggaa gtcaagagtg gtactgtgat aaagatggca ctaatctaca
     1441 ctggccagta gatggatatt ttgaagaagg agtacgtaag tcaaattttc taggagaaga
     1501 ggttataaaa tatcataaaa cattaacaac ttatttaaat acgttaatga aaacaggatt
     1561 tgaaatatta gaaattattg aacctcaacc agaagaaaaa ctgttagata caattcctga
     1621 tatgaaagat gagctacgta gacctatgat gttacttgta tctgcaaaaa aaagataagt
     1681 aaatataaat aatcattatt gtagaaaaaa agttgtagaa aataattaca ctaatggaga
     1741 tgattatacc agtggtatta attacaccag tagtaaggag gcaaaagcat tcgttgcttt
     1801 tgccattttt taagttgcaa ttatacttct acgtagtata tttcgtttcc attttcttct
     1861 ataagctttt tcatatcttc aaattttaaa aatacagtag cagtattatc atttggatgt
     1921 atacctattt tatccatttt taataaatct ttatcaaata caacttttac aacagcctct
     1981 ttatcattta atataccaaa aggagtcaca gctcctttag taagtttcaa gtatttttga
     2041 agtctttctt ccgatgcaaa actaagaggt ttactatcaa tttgttgacg aatatctttt
     2101 aaatctgctt ttttgtcttt acctaataca actaaaaaat gttcttttcc tttagcatca
     2161 cgaagaaata ggttttttac aacaaattcc atatgtaatg tttttaattc ttccatttct
     2221 tcaattgtat aaactggtgg atgatttaca gcctcataag gtatgcttaa ctcatctaat
     2281 tttttatata cttcctgttg ttgattgttc atgatttcct cccattagaa atttatttta
     2341 tatataaata atatt
//
LOCUS       DAGSHT010000323         2354 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.329, whole genome shotgun sequence.
ACCESSION   DAGSHT010000323 DAGSHT010000000
VERSION     DAGSHT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.329"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1091)
                     /locus_tag="MJB72_003593"
     CDS             complement(<1..1091)
                     /locus_tag="MJB72_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY8264651.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLP"
     gene            complement(1646..>2354)
                     /locus_tag="MJB72_003594"
     CDS             complement(1646..>2354)
                     /locus_tag="MJB72_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY8264652.1"
                     /translation="IRDKQVPKGRILNATISKEPSGRYYVSLCCTDVDIEAFENTNNH
                     IGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLEVAG
                     LQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQ
                     LEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINA
                     SINILKEGLRLITTQNK"
BASE COUNT          711 a          443 c          252 g          948 t
ORIGIN      
        1 ggtaagtgat atctttgcca atattcattt aaggcaactc taagacctga tgggtctatt
       61 gctgctccat actcagaggc ctctaagtta ggattcattg acattttaaa tagtccatac
      121 atactaaaat caacagttcc ttctcttaat ccaaatggat gttgtgaatc ttcaggtaaa
      181 gcttctgcac aatttgtcct ataataattt acagctataa aatccatttt agctttttta
      241 agtatctctt tatccccatc ttccatgtga ggaactatat ttcttttttc aagatacttc
      301 atataatacc ctggatattc tccataatga tgcatatcaa gcatatagta cactttaaaa
      361 ttatcattca ttctagctgc atatacatct tctggcttag aagtaagagg atatgttaca
      421 gatgatgata ctgctgctcc aattttactt tcttttatca tctcatgaca tgcattgact
      481 gctaatgcat gtgctaagca aagattataa tccatctgag cacgcatttt atctatcttc
      541 catgaatctg tttcatcata tatattaatt ctctcctcca ctctaacaat taaattctgt
      601 tcattatgag gttgccaata ctttacccta tctccaaaat gcttaaagca tattttagca
      661 aatctttcaa aagcatagac agtttctcta ctttcccaac cattatattt ctctactaat
      721 ttaaatggaa ggtcaaaatg gtataaagtt acgaatggct caatatcaca ctctattagt
      781 ttgtctatta tcttattata aaagtctaaa cctttctggt ttatctctcc ttcaccatct
      841 ggtattattc ttgcccatga aattgaaaaa cgatatgttt tcatccctaa ttctttcatt
      901 aattcaatat cttcttcata attatggtaa aagtctgatg cgactttagt atctgcctgt
      961 aaatgagact ttttaaaaga attatagtcg gctacggttt ttcctttatt gtctttatcc
     1021 caagctcctt ccacttgaaa tgcagaagat gatgctcccc ataaaaagtc ttttggaaat
     1081 cctgtattca taaatatttc tcctttttat attgattatt ttctttgttg attcattgca
     1141 ataagttcac tctatattaa ctcttttctt tgttatttca ttgagataga tttaccctat
     1201 attaattatt ttctttaaca attcattaca ataaacttac tctatattaa ctcttttctt
     1261 taacaattca ttacaataaa cttactctat attaattatt ttctttgtta cttcattgca
     1321 atagatttac tccatattaa ttattttctt taacaattca ttgcaataaa cttactttta
     1381 gatgtattta attgttgatt tacttatttc cttttaaatc aatttactta tctctagtca
     1441 aattaactta attaccagct aatactaatg aaattatatt atcattggtt gaaacataac
     1501 tagatttaca agaaactact tgcccaaact gaacctccca tgactaaagt cacagggttc
     1561 ctgcttcaca gaattttgca tactaaaata tgtcttacta attctccaca ggctatcccc
     1621 gtagttccta cggttcttac ataagttatt tattttgagt tgttattagt cttaatcctt
     1681 cttttaatat atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac
     1741 aaatccattc tcttatattt aaatccttaa cttcctcgtt tttataccca catttattgc
     1801 atatttgtga acttgcaaag aatttaccca cctttacaat ttgtctacca tgccaattag
     1861 ccttgtattc taattgacga ataaactctg accatgacac atcagaaatt aagcgagata
     1921 gtttacgatt tctaatcata ttctttactt gtaaatcttc tatgcaaata atatcattct
     1981 cttttattaa tttagtagat agtttttgta aaaaatcctt tctttgatta gctatatgtt
     2041 cttgaagtcc tgcaacttct aatcttgctt tatttctatt taaactaccg attgtttttc
     2101 tcgataattc tctttgtaat ttagcaagct tgtttaatga cttcttaaga tacttaggat
     2161 tttctataaa ttctccacaa cttgagatac aaaattcttt aattcctaaa tctaaaccta
     2221 tatgattatt agtattttca aatgcttcaa tatctacatc agtacagcat aatgatacat
     2281 aatatctacc acttggctct ttagatatag tagcattaag tattctacct ttaggtactt
     2341 gtttatccct tatc
//
LOCUS       DAGSHT010000324         2339 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.330, whole genome shotgun sequence.
ACCESSION   DAGSHT010000324 DAGSHT010000000
VERSION     DAGSHT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.330"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..906
                     /gene="cas8a1"
                     /locus_tag="MJB72_003595"
     CDS             <1..906
                     /gene="cas8a1"
                     /locus_tag="MJB72_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBY8264653.1"
                     /translation="CTPAGATYTRKNYLINEENEFYLFVNMDTSINELFERNNSLKVQ
                     KSESSDSKDENPFNQLIKSIVEENTLKSEWQLRNILFVEFKASIDSKKCKINYFNIPT
                     YLAKFFTDKYANKKIQSIYDYKLKSNVLDLLLKNRDLKHLINNILRNKVKNDMESNNK
                     SNISGIDCFRVVQLRALINNYKKGVYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKN
                     SKNKIGGVSYKLLNSVKVGNKSDFMDTIIRVFMSAEKQMPAFILDIEIEKDLDFESIG
                     HAFISGLISGKYEGKDKLPNEKEEK"
     gene            908..1822
                     /gene="cas7i"
                     /locus_tag="MJB72_003596"
     CDS             908..1822
                     /gene="cas7i"
                     /locus_tag="MJB72_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBY8264654.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            1824..>2339
                     /gene="cas5b"
                     /locus_tag="MJB72_003597"
     CDS             1824..>2339
                     /gene="cas5b"
                     /locus_tag="MJB72_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBY8264655.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQ"
BASE COUNT         1005 a          197 c          404 g          733 t
ORIGIN      
        1 tgtactccag caggggctac ttatacaaga aagaattatt taattaatga agaaaatgag
       61 ttttatttat ttgttaacat ggatacttct ataaatgagc tttttgaaag aaataatagt
      121 ttaaaagttc aaaaaagtga gtcttcagat tccaaagatg aaaatccttt taatcagctt
      181 ataaaaagta tagtagaaga aaatacatta aaaagtgaat ggcaacttcg taatatactt
      241 tttgtggaat ttaaagcaag tatagattct aaaaaatgta aaataaatta ttttaatatt
      301 ccaacttatt tagcaaaatt ttttacagac aagtatgcaa ataaaaaaat acaaagtatc
      361 tatgattaca agcttaaatc aaatgtacta gatttactct tgaaaaatag ggacttgaag
      421 catctaatta ataacatttt aagaaataaa gttaaaaatg atatggaaag caacaacaaa
      481 tcaaatatat caggaataga ttgttttaga gtagtccaat taagagcatt aataaataac
      541 tacaaaaagg gggtatataa aatggatagt aaaaatttgg agaagaatga tgaaaagctg
      601 agaataattt attatttagg ttgtgatata catgactatt ttgttaataa aaattctaaa
      661 aataaaatag gtggagtaag ctataaactt ttaaattcag ttaaagtcgg aaataaaagt
      721 gattttatgg atacaattat aagagttttt atgtcagctg aaaaacagat gccagcattt
      781 attttagata tagaaattga aaaagattta gactttgaat ctatagggca tgcttttata
      841 tctggattaa tttctgggaa atatgaagga aaagataaac taccaaacga aaaggaggaa
      901 aaataagatg aaaagagggt taacatttac agtaatagca caagctcaaa gtttaaacta
      961 tggtgaaggg ataggtaata tatcagaatt aaaaaaatta agtagagatg atggaaatat
     1021 atatacattt gcatctcgcc agtgtttaag atatgacata gtacgattag cagcagaatt
     1081 gtttaattgg aatttacagg ttgtcgataa agaaaaaggt actattcaat ttaaagataa
     1141 tataagtata agagaatcag aagaaatgga tttatttgga tatatgaaaa caaataaaaa
     1201 agatgagaaa gataaaaaag gaggttcttt gactagagaa gctactgtaa gattaagtaa
     1261 tgcgatttct cttgagccat atagaagtga tatggatttt ttaaataata aaggatttgc
     1321 agatagaata ggagaacacc ctaatcttgc taacatagag caacatttaa gttattatac
     1381 atatacagtg actatagatt tatctaaaat aggtaaggat ggagatatag aacttgataa
     1441 taaagagaaa tgtagaagag tagtagaatt tttagaaata ataaaagtat tgaatagaaa
     1501 tattagaggt agacaagaaa atttatctcc tctatttgta gtaggaggag tatatgaaat
     1561 agcaaatcca tttttcttgg gtagaattaa attaaaagga gataaaaatg gatttaaaat
     1621 aaataagcaa gctatagaag aagtaattca aggtactttt ttaggaaaag atttaaaaga
     1681 atttacctat gttggaatgg tagatggggt atttataaat aaagaagaat ttaagggatt
     1741 gtttgaagat aattttttaa gtgtagataa attttttgga caactagtta aagaagtaaa
     1801 ggaatattat ggggtgaatt aatatgaagg ttttgaaatt agatttattt caagaaactg
     1861 catgctacaa aaaaccattt gctatgaaga ttacagagac atatccactg cctccatatt
     1921 ctacaataaa tggattaatt cacaaaatat taaatgctac agaatatata ccatttaata
     1981 taagtgtaca gggaacatat gaaagtatat ttaataatta ccagacaaca tatttttata
     2041 aaaaagatag tattacatct atgccaatga atagtcatat gctattgaat gtaaatttga
     2101 taattcatat aggtggggat tttgatttat tggaagaaat atatgatagt ttattaaatt
     2161 atgacgagca cttatcatta ggtagaaaag aagatttagt aagaattaat gatattagat
     2221 ttgtagaagt caataaattt gaagtagatg aggatataca attagatgat tatgaaaatt
     2281 ataagttatc agaatgtaat ataagaatgc ctatatatat accaaaaaca agtttacaa
//
LOCUS       DAGSHT010000325         2335 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.331, whole genome shotgun sequence.
ACCESSION   DAGSHT010000325 DAGSHT010000000
VERSION     DAGSHT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.331"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            290..2185
                     /locus_tag="MJB72_003598"
     CDS             290..2185
                     /locus_tag="MJB72_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBY8264656.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKRYSDIDSKELHTITVKN
                     ADLQDISAEKLFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISISSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1027 a          239 c          430 g          639 t
ORIGIN      
        1 agtaaagcaa aaattagtgc atagtagtat ataaaaatgg agtttgaata cttttgttag
       61 tatatgattg tttagtagaa aatcactata aaataggatt tctatctttg agcataatta
      121 tagagagcct aagtaggggg aagggtttca agaagatatt attagtgttt gtgttagtaa
      181 attatgtaaa cacataaaat atattgaaaa ataatattat atataatatt atttatgtgt
      241 gggaaatacg aatataaaaa ataaaaaata atattcggag gacataaata tgttaagcaa
      301 caaaaagaga agtatggcaa tagtaatggc aggtgctaca gttatgagtg cagcagcacc
      361 aatattcgca gacaatactg tgacagaaaa tgttgataaa aattacacag taagtgcgaa
      421 agattctgct aaactaatcg aagaggttag aaaagcatta gaagttaagt ttgaagatac
      481 aaaagcagga gcaaatgtaa atgatagagt ttacgatata aaagtagaca atgtaaactt
      541 aactaatgca actcaattac aaaataaaat aaactcttta acagaagggc aaagtttaaa
      601 agttactatt caagataaag ggcatcaagt gttaggtgga aaagtagttg actataaaat
      661 tgaaaactac aaaactgctc aagaaatagt agatgcagtt aatgcttaca atgcaacttt
      721 agcagaagat tctgataaca agttaacagc aactataaaa tctacaaata cagttgaagt
      781 aaaaagagca aaagattcag caaatgttat tacattaaat gtaggagacc aacatttaga
      841 tttctcaaaa gtgataacat cagaagaagg tacttttgaa ggttatgaaa agcgttatag
      901 tgacatagat tcaaaagaat tacatacgat aacagttaaa aacgcagatt tacaagacat
      961 atcagcagaa aaattatttg atggaattag attaactaca ttaggaagag aaatagttaa
     1021 taaagttaaa aatggatatg ctttaacatt tgaaaatgaa gcaatcctta ctcaagaaca
     1081 agaagatagt gatgataaag ataaaccaga aaaatcaagc tttgatatag ttttaagtaa
     1141 ggcaaatgaa aagccagaaa caataagtat ttcaagtaaa aaccataagt tagttagaga
     1201 cttacacaaa gtattaactg atgtaaaaga tggaaaagaa ttaaaagtag aagttttatc
     1261 tggtgattca agatttacaa cagcagtaga agttagtaaa gagagattca aagatggaga
     1321 agcagaagct ataattttag ttggagaaga tgctatagtt gatggattag catcagcacc
     1381 acttgcctct caaaaaaatg caccaatatt attatctaaa aaagattcac taccatcaga
     1441 aatagaagct gaaatattaa gagtacttgg aagtaatcta tcttctaaga aaatatatat
     1501 agtaggtgga gaatctaaag tatcaaaaga aactgaagaa aaactttcta aattaggtgt
     1561 aagtaaagtt gagagagttt ctggagaaga tagatttgaa acttctttag aaatagcaaa
     1621 acaattaaaa gatacattta agactgcttt tgtagtaggt ggaaatggag aagctgatgc
     1681 tatgagtata tcagctagag ctgctcaatt tggtgctcca ataatagtta caggtaacga
     1741 attagatgca aatgctgaaa aattattaaa aggaaaagaa ttagaaatag taggtggaga
     1801 aaattctgta tcaaaagaag ttgaagacaa attagttgat atagatttaa ataataaagt
     1861 tgaaagatta gctggagaaa atagaaaaga tactaatgct aaagtaatca ataaatacta
     1921 tgcaggtgca actaaagcat atgtagcaaa agatggttat gtaggtggaa atggacaatt
     1981 agttgatgca cttacagcag caccacttgc agctagttca aaggctccaa tagtattaac
     2041 tacagaagaa ctttctaaat cacaagaaga agtagttgag ttaagactta agaatgctac
     2101 taagttagta caaataggtg aaggaatagc taaaaacgct attgaaaaaa tagcagaaaa
     2161 aataaactta tttactaaga actaataaga tataggaatt aatattaaaa gtttatttag
     2221 ataatatatg attatacaat gcgttaatat gtaagatata gtgtagatat tataaaatat
     2281 acaaataaca agataaaata ataaaaataa ggtgtctcat ttagaattag attga
//
LOCUS       DAGSHT010000326         2326 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.332, whole genome shotgun sequence.
ACCESSION   DAGSHT010000326 DAGSHT010000000
VERSION     DAGSHT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.332"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            100..1551
                     /locus_tag="MJB72_003599"
     CDS             100..1551
                     /locus_tag="MJB72_003599"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBY8264657.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCFVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(1642..2310)
                     /locus_tag="MJB72_003600"
     CDS             complement(1642..2310)
                     /locus_tag="MJB72_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBY8264658.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT          948 a          232 c          411 g          735 t
ORIGIN      
        1 atggattgcc tagatataga taataaagat aaattattta cttctttgga agagtctaaa
       61 aatataatag aacaattatt ttaaaaagga gaatttatta tgaatataaa gtattctaaa
      121 gagatattaa aaagtttaga aaatgctata acagtagtag ctctatttga gggagaagat
      181 gtttttacga catttgataa aataaatatg ataagcagaa attttaattc taaagaaaat
      241 agtatagata ctgtaacatt aattgaaaat aacaatttgg taaatgtttg ttttgttggt
      301 tttggtaaga gagagttatt gaatagagaa aaaattagaa tcataggtgg gaatttgtgt
      361 aaaaaattaa aaaatatatt gaaaaatgaa gcatataaga gtagtgatgt taatgtactg
      421 aatcttaatt taaatagtga tgaaataggt tgttttgtag aagggatatt acttggaaat
      481 tataagtttg ataagtataa gacaaaaagt aaggattttg aaagaaaaga agttgatact
      541 ataactatat ttactgaaaa agaagttgag tgtcaaatag aaaaggctaa aatattagct
      601 aattcaacta taatagctag aaatctagtt aatgaaccat caaacgtaat atatccaaaa
      661 acattagcag tagaaacagt taaacttggt gcagagtttg ggtttaatgt tgatgtttat
      721 gaagaagaaa aaataaaggc attgggaatg gatgcttttt tcgctgtttc aagaggttca
      781 attaataaac caagattcat agtaatgaga tactttggag ataaagaaag tacagatata
      841 ttaggtctag taggtaaagg attgacttat gatactggtg gatattcttt aaaatcaaat
      901 gcttctatgc tagatatgaa gacagatatg gctggtgctg cgagtgttat aggtgctatg
      961 tgtgctatat ctcaaagtaa attaaagaaa aatgttatag cagtagtggc tgcatgtgag
     1021 aatgctttat caggaggttc atacaaacct ggagatataa ttagttctat ggcaaagaaa
     1081 actattgaag ttttaaatac ggatgcagaa ggtagactta cattagctga tgcaatatac
     1141 tacattataa acaatgaaaa agtaacaaag gtggttgatg tagctacatt aactggtgct
     1201 gctttaacat tgttaggtaa tgttgcaact cctatagtaa caaataatga tgatttttat
     1261 tgtgaattag aaaaagctgc cactttatca ggggaaaggg tatggaagat gccaatttat
     1321 gatgaattta aagatatgat aaaaggagaa gaagcagacc ttaaaaacac tggtggtaaa
     1381 aatgctggat gtataacagc aggagctttt attggtgaat ttgttgggaa tactccttgg
     1441 atacatatgg atatagcggg gacaagtaca tcatcaaaat caattggata caaagcaaag
     1501 ggggctacag gagaacctgt tagaacattg tactatttgg cagaaatcta aaaaacaatt
     1561 ataaaatata atttataaag ccacgtggat atttttgtaa tttaagatta tctatgtggc
     1621 tttatttttt atttaagtaa attatttact ctttttcttt ttttcaagta taaaagtatc
     1681 tctattaaat aaaagagcag gtatgtacat aatactagta acaaaaataa acgtaaaaat
     1741 agcagttgtg ctatgactta aaaaaccata atagtaccta gagttggtaa atgtgtttag
     1801 taaaaatatg aatgaaaaat aaataaaaca tatagctaag ttatctctaa gtgcatatct
     1861 gttaactcta aattttctaa catcaaccat atatatatct gcataaatta tcaagcactt
     1921 agagaagata aagataaaat atattaagct aggaaattca tagcccaagt ttttagaaaa
     1981 aatgagttct ccaactgaac aaactaaact ccaagaaaaa aatatattaa aaagatgata
     2041 ctgcttaaaa attagtatgg ctatacacag atatatacaa atataattga ttccaattgg
     2101 taaagagttt agagtactat aattacctga agaaataagt aataattgct ctagagccat
     2161 aattattaca agtaatgtac aaattatctt ctcaactgta tagctataag gtaaaatatt
     2221 tttggttagt tttggacaat agtataaaaa taatccaaat ataaagagac ttacaaattg
     2281 ttcactagaa aaaagaaaaa ttttaaacat ataacattct ctccta
//
LOCUS       DAGSHT010000327         2291 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.333, whole genome shotgun sequence.
ACCESSION   DAGSHT010000327 DAGSHT010000000
VERSION     DAGSHT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.333"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..299)
                     /locus_tag="MJB72_003601"
     CDS             complement(<1..299)
                     /locus_tag="MJB72_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264659.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTL"
     gene            complement(406..909)
                     /locus_tag="MJB72_003602"
     CDS             complement(406..909)
                     /locus_tag="MJB72_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264660.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            complement(961..2160)
                     /locus_tag="MJB72_003603"
     CDS             complement(961..2160)
                     /locus_tag="MJB72_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBY8264661.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
BASE COUNT          742 a          449 c          156 g          944 t
ORIGIN      
        1 cttaatgtag aaaaaactat aactacagtt aaaagaagtc tagcatataa aaaatttttt
       61 tctttcaaca taactcctgc aactccaaat ataaaaaata gagaagcaat aaattttcca
      121 ataagattat tattaggata ataactaaaa agcataaaac aaagtacaaa tgtaagtact
      181 cctattgcat taaacacaat tttattagat gctatttttc ctttattgaa aaagaaacag
      241 aatattgaca ctaagtctaa aaataaaaat acataaaaca ttccttttaa aacgcccatt
      301 tttatacccc ctaaaaattc taattataat atccagttta tttcatataa tcgtcaatat
      361 aatcaatact ttataacatc agtataacag tattacaaat ttatattacg aatgtattac
      421 ttcgtattca tacatattat aatcacactc atacatattt aaagactcat atgttgcttt
      481 tgcatttatg ttttcttttt ctacatataa tctcattcca actatatttt catctttatc
      541 acatatattt tttatataat taaataaata attaaagata ccttttcttc tatattcctt
      601 atctacatat acactctgaa tccaaaggaa atttccattt ctccaatcac tccactcata
      661 tgtatacata atctgtgcaa caactttatc aaatactgta tatacatgat attttccttt
      721 tctctcatct aataacaatg cttttacacc tttagttaaa acatccatat caagttcttt
      781 tccttcagtt tctttagcca aattataatt aaattttgct attgtatcca aatcatccat
      841 acgtgcttct cttatctcta ttagttcttc attttcatat aactctatgt agtccttttg
      901 ttgctccata cttattcctc catatctatt ttatctacta aggtttttta tataatatat
      961 ttatagagtt ttttctatct tcataaatgc ataatcaagt gtagcctcta aatttccatt
     1021 attcttacct cctccttgag ctaatacttt acttccacca cccttgccat ctataagact
     1081 tattgcatcc tttaataaaa ctcccatatt aatatctttt aaatttttag aacatgcaaa
     1141 aactaaattt attttatcat taaatttaac agctaataat gcaatactat tttcatattc
     1201 tgcaatttta tttgctatct tatttacata tttcaaatct tcatcatcat aaattttttt
     1261 aatgaccgaa atattgccaa ccttatttgc tttttcaatc atttctttga cctgatatgt
     1321 agatactact tcttcaagcc ttcttttttt aattaaagcc tcttccactt tactatttag
     1381 atttacaata ccatttatag catcttcctc actacagctt aaatatctac atattttagt
     1441 caaatatata tccctcttca atacttcatc cactgccctt ttacctgcta gaaactctat
     1501 tcttgtactt gacttatgtt tttcccattt ctttattttt atcattctca attcaccagt
     1561 tgattttgga tgcactccac aacatgcatt cgtatctaaa tcacctattt ttattactct
     1621 tatctcttca tcagtatttg gcaaatcacg tcttaaccaa atctttttta gttctttttt
     1681 cgttggaagt aatgtctcta cttgtatatt ttgacgaata atttcattgg caaacaactc
     1741 tatctctcta attttatcct cttctaaatt accttctata tctactgtac tatactcatt
     1801 tcccaaatga aaacctgtcg tatttgcatt aaaagtttta aaaaaacatc cagacaatac
     1861 atgttgtcca aaatgttgat gcattccatc ttctcttcta tcccaatcaa tactacactt
     1921 taccttatgt atctttatag gttttttatc taatacatgg tatatatttt ttccatcttc
     1981 atacacattt attacatcat cactctctat ttttcccagg tctccaaact gacctcctcc
     2041 accaggaaaa aaagctgtct tatctaataa aacatgatac ttattttcta cttcttttat
     2101 ttcaattatt tctgctgtaa aatctcttat atactgattt gtataatata atttttccat
     2161 tataattcta tctccatttt cttaattcta gattacttta ctttttagat acaaatttca
     2221 aactaagctt aatttaactg tattaaaata gaactcatcc tattatttac taacatcttc
     2281 tactatattt t
//
LOCUS       DAGSHT010000328         2275 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.334, whole genome shotgun sequence.
ACCESSION   DAGSHT010000328 DAGSHT010000000
VERSION     DAGSHT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.334"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..593
                     /locus_tag="MJB72_003604"
     CDS             <1..593
                     /locus_tag="MJB72_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8264662.1"
                     /translation="KIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLE
                     EIRFEKFESKDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHC
                     MSERIDIMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNI
                     IFTNKINKFSIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            623..1045
                     /locus_tag="MJB72_003605"
     CDS             623..1045
                     /locus_tag="MJB72_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY8264663.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            1064..2215
                     /locus_tag="MJB72_003606"
     CDS             1064..2215
                     /locus_tag="MJB72_003606"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY8264664.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEGILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
BASE COUNT          978 a          200 c          406 g          691 t
ORIGIN      
        1 aaaaaataaa atctatagct caaaataaat taatcaattt aaatgcgaaa aatgatacta
       61 agagtaagaa taaagtatat gaacagataa cagaaagtat gaatatagga aaagatattc
      121 ttcaattttt agaggaaatt agatttgaaa aatttgaaag taaagattta agtgagctta
      181 ttgaagactc atcaaaaata tttgcaaaat ctagtgaaga tataatctct ataaaagaat
      241 cgttaaaaga aagattaaaa atttcaattc catatataga agaatcaaaa attctattgt
      301 tacattgtat gagtgaaaga atagatatta tgaagttatc aataataaaa ctagagaata
      361 gaatcgtgct agattcaaat gaagaaatcg acaatgtagt ttttatgtta cttcctaaaa
      421 attcaccaag ttatcaaaga caaataatga gtgaaataag tggttcatta atagacaata
      481 taatttttac taataagata aataaattct ctatagaaga gatggcatta gaggtaaaag
      541 atatagtttt taatttttat acaaacagat taaaagcatt tatagataaa taaaattaat
      601 attaaaaagg aaggttgata ctatgagtaa agtattgaat gagaataata tatttttagg
      661 attagatagt gtttcaaagg aagaagctat aactttagca ggaagaaaat tagttgaaaa
      721 tggatatgta aaagaagaat atataccagc aatgttagaa agagaaaaag taatgactac
      781 atatatggga atgggagttg ctattccaca tggtgtaaat gaagcaaaaa aagaaatatt
      841 atcttctgga atagttatat tacaattccc taatggtata gattttgatg gagaaaaagc
      901 atatttatta ataggaatag ctggtgttgg agatgaacat ttagaaatac tatctaatat
      961 agcagtagtt ttagatgatg acttaacaga aagactaaaa aattcaaatg acaaacaagc
     1021 ttttatggaa gcttttgcca actaatctta ggagggattt aaaatgaaaa aggcaattca
     1081 gtttggagca ggaaatattg gaagaggttt tattggaggc ttgttagtta agtcgggtta
     1141 tcatgtagta tttgctgatg taaatgaggg gatattaaac tcaataaaca aagataaaaa
     1201 gtatactata cacataagag atgtagaatg tatagatgaa gtaattgata atataagtgc
     1261 ggtttcatct ataaaagaag aaataataga tgagattgta caggctgaaa taataactac
     1321 agcagttgga cctttagttt tgactaaaat agcctctaca attgcaaaag gtataaaggc
     1381 tagaaaagaa aagggattaa caagtaatct aaatataata gcatgtgaaa atgcaatata
     1441 cgcaagttct tctttaaaag aagaggtact taagtattta aataaggaag aagtagaata
     1501 tttagagatg tatgttggtt ttccaaactg ttcagttgat agaatagttc ctcctggtaa
     1561 aaacgaaaat cctcttgatg taacagttga aaatttttat gagtggaatg ttgaaaaaca
     1621 aggatttaaa ggggaaatac caactatagt tggaatgaat ttagctgata atcttatggc
     1681 ttatatagaa cgtaaattgt ttacactgaa tacaggtcat gctataacag cttatatagg
     1741 atatttaaaa ggatataaga ctatagaaga gagtataaaa gataaattta tctgtgatat
     1801 agtcaaaagt gcaatggtag aaagtggaga aggattgatt aaaaaataca attttgactc
     1861 agaagtgcac tataaatata ttgataaaat tttaaataga tttaaaaatc catatcttaa
     1921 tgatgatgta ttaagagtcg gaagggaacc attaagaaaa ctaagtgata aagatagatt
     1981 gattaaacct ttaatgacag caaaatcata tggattatca gttgataatt tgattcttgg
     2041 aataggtgca gcgcttcatt ataataatag tgaagatact caaagtgtag aattacaaga
     2101 gttgataaag tctattgggg taaaaaaagc cgttgctaaa atagctaata taagcaatga
     2161 cgaagaactt ttaaataata tcgaaaaaag ttatatattt atgaaaaatt tataaattta
     2221 cttgaaaaaa agtagttctg ataaagtaac tataatatat tattctacac agttt
//
LOCUS       DAGSHT010000329         2260 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.335, whole genome shotgun sequence.
ACCESSION   DAGSHT010000329 DAGSHT010000000
VERSION     DAGSHT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.335"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1709
                     /locus_tag="MJB72_003607"
     CDS             <1..1709
                     /locus_tag="MJB72_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8264665.1"
                     /translation="QNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKD
                     YKFLARFYSQIAILSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASK
                     CIESSVIIKEIETLYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKY
                     ADLLKDMQVSSLCNIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNI
                     TKILIKTRNYPVAINNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQ
                     SICYLDRAKNILNMIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKN
                     AKNYNYVKLAYKSIYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHE
                     YIEKEINNMRKVKLDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFK
                     EYNDTYGHYNGDIALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRK
                     LCRIVKKFLNNNLTVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFK
                     NF"
     gene            1736..>2260
                     /locus_tag="MJB72_003608"
     CDS             1736..>2260
                     /locus_tag="MJB72_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY8264666.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVS"
BASE COUNT          992 a          171 c          321 g          776 t
ORIGIN      
        1 aacaaaatag tttaatgtta ttaaaatcaa attttttgct agctttatta tatggtattc
       61 aagggtttag tgaaaaaatg aaagagtata ttttgatttc ctgtaaatat attgataaat
      121 gcttacccaa agattataaa tttttagcac gattttattc acaaatagct attttaagtt
      181 taaaaaatga agatgtaaat aaggcaaatt tatatatgga taaatttaat cttatttgtg
      241 atgaaaataa ttttctaatt gaagaaatta tttttaaatc acaacttatt tacattaagg
      301 caagtaagtg tatagaatct tctgtaataa ttaaagaaat agaaacgttg tatataaaga
      361 taaaagaaat taacgatttt aattgtaaaa aaatatattt ttttattatt ggtaaaatat
      421 attttttgtt tttggatgat gctctaattg ctaaggtaaa ttttttaaaa gcaagaaaat
      481 atgcagattt actaaaggat atgcaagtat cttcactatg taatataaag ataggtgagt
      541 gtgaatgttc gtttgaaaat tatgaatgtt ccatggaata tttcaatgaa gtaataagca
      601 ataaaaagta tagaaatgta aatataattc aaaaatatag agcatcaaac aacataacta
      661 aaatattaat aaagactaga aattatcctg tcgcaataaa taatttagca aaatctgaag
      721 tatatctaga aaaaattaaa aatcatagct taaaagaagt agagaagttt gatttattta
      781 taagtttagc catgtattat gcaaaaagta atgaaaaatc ttttgaacaa tcaatatgct
      841 atttagatag agctaaaaac atattaaata tgataaaaaa tatagaagaa tatataatat
      901 atgatttaga aataatttat caccaggcaa agatatatta tatctttgga tattatgaca
      961 atgccttaaa tacaaataaa aaactccttg aaaaatctaa aaatgctaaa aattataatt
     1021 atgtgaagtt agcatacaag agtatatatt tgtgttttga aaagattcaa aattatgaaa
     1081 tgagtatgaa atactttaaa atgtattatg agttaaaaat gatgtatata aaagctcgaa
     1141 atagaaatta tatagattcc ttaaattata gacatgagta tatagaaaaa gaaataaata
     1201 acatgagaaa agtaaaactt gatttagata aaaaaaagta tgttgaccat ttgacaagtg
     1261 catataatag aacttttttg aacgattttt tagaaaaaca agaagttagt gaatattgca
     1321 cagcatttat gatagatgta gattacttta aagaatacaa tgatacttat ggacattata
     1381 atggagatat tgctcttaaa aatataacta taataataaa aaaatatcta aaaaaagaca
     1441 tgcttatgat aaggtatggt ggagaagaat ttctgatatt atctgtatgt aaagattata
     1501 aaaaaagtaa aatatttgga agaaaattat gtaggattgt aaagaaattt ttaaataata
     1561 atttgacagt tagtattggc attgatacat gtaaaaatag tagtatagat ataaaagaaa
     1621 taattgaaaa tgctgataaa gctttatata aagcaaagga aagtggtaga aatagatgtt
     1681 tgcattatca tgattttaag aatttttaac tgttgaaata ggattggtga tttacttgaa
     1741 aaggagttta atagaagatt taaagagaaa tataaacaag atagaatacg atatgttgac
     1801 taaattctat ctaaatttga cagatttaaa ctacatcttg gattatcaca aagacctttt
     1861 aaatacaaaa ttagaagata atagtagagt ctatacttta tatttaatta gtcttataaa
     1921 ctatataaaa aaagatagag aagcatcata caactatttg atagagtcat taaagtatat
     1981 taaaaaattg tcattaaaag atgattcaga tataatatct aaaatttata tttatcttac
     2041 tttatcagct atttctataa gaaaatatga taaggtaaat aatttttatt tctcagcaaa
     2101 aaaaattata aggcaaaatc atttaaatga gttgttggta ttgttaaata tgagtttatg
     2161 tgtggaaata agtgctttat atcagaccaa aacggatgta atagctttag tagaagaagt
     2221 gtcttttttt aaaactttaa aaaataaagc tcttgtaagt
//
LOCUS       DAGSHT010000330         2252 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.336, whole genome shotgun sequence.
ACCESSION   DAGSHT010000330 DAGSHT010000000
VERSION     DAGSHT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.336"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..752)
                     /locus_tag="MJB72_003609"
     CDS             complement(<1..752)
                     /locus_tag="MJB72_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8264667.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLEGLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLN"
     gene            894..>2252
                     /locus_tag="MJB72_003610"
     CDS             894..>2252
                     /locus_tag="MJB72_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY8264668.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQIGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLAMSFGFFLMSRLKESSDLILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVT"
BASE COUNT          771 a          314 c          285 g          882 t
ORIGIN      
        1 atatttaaat ctgtttcctt taaataatat atagctttat caagcctgat tccatttata
       61 aaatcgattg tagtcttacc tgttatttgc ttaaaggttc tacaaaagtg atgtgcactc
      121 aagttcacac gactagcaag ttcttttaat gtaattttat ccatatattt ttcttctatc
      181 aaatcaaaga tacattcaaa tttttctaaa gaatactttc tttttataaa ttcatcttca
      241 gaatatattt tatcaatatg acttcttaat aagagtacta ttaattgata gatattagct
      301 tttatagata actcatatcc aacttcttta ttgtgatatt catcaagtat agttgtaata
      361 cattttaaaa tcagagaatc attttgaatt aaattattaa atacaatatg atttaaagcc
      421 aatggtgcta agtacttggt ttgtaacaaa tcaacttgat tactaaaaag gaatgtgggt
      481 tcaatacgaa tactatagaa ttttaaatta tctgataagc cttctaaata atgtaattca
      541 ttactgtttg ctattacaat atttccaggc actaccttaa aattatccaa tccacattca
      601 agatatgcta taccatcaat aaaataatat atttgtagat gctcatgcca atgtctttta
      661 aagtttgctc cttttttaat gtcatccttt aaaaaaatat ctataaagtt ttcttgacct
      721 ttcctgttaa aatatgtatt tttatccttc attattcccc cctcatcaaa atagtgctaa
      781 ttataagcaa tatagttatt gtattcatta tttcatatcg ctattatata aaatgtaact
      841 atcattgtaa acattaacat aatgccagtc attataataa ggaggtaata aaaatgacaa
      901 actcttttaa aaaagatgat aatagaaaaa aaatcacagt tttatttgta gtcattgcca
      961 tgacttttat ggcaacatta gattctagta ttataaatgt tgctctccca gttcttgcaa
     1021 gcaaactaaa tgtatctcta gcatctattg aatgggtaat agcaagttac tcaattatta
     1081 tttgttcaac actattattt tttggaagac ttggagatat tattggtaaa tcaagagtat
     1141 ttcaagtagg tactattctc tttacatcag cttcattact atgtggatta agtaattctt
     1201 taactttact tatagtttgc cgctttattc aaggaatagg cgcttctgcc tacatggcaa
     1261 acaatcatgg aattatcaca gaactatttc caaaagaaag cagaggaaaa gccctaggga
     1321 ttttagttac tgcagttgct attggaaata tggttggtcc atctgtggga ggatttattt
     1381 tgtcaatatt tgattggaat gttatatttt ttataaatat tccaatcggt cttattgtaa
     1441 tctttttaaa tacaaaattt cttcctaata gtaaaaaatc ttcagaaaat atggacaaaa
     1501 ctggtgctat acttcaattt ttaggaacaa cattattttt tagtgctcta attagtgctc
     1561 aacaaatagg attactaaat ccatatatat taattgcact attattatca attattttta
     1621 ttatattatt tttgatatta gaaaaaaaac atcctcaacc gcttcttgac cttgaaattt
     1681 ttagaaattt taaattttca ttgaatttaa tttgtgctct tacatcattc atttgtattg
     1741 catcatcaag tattttaatt ccattttatt tacaaagtac tatgaaactt ccccctattc
     1801 aagcaggttt atttatgata ctatcacctt tgattttagc cattttttca cctatatttg
     1861 ggaatatatc agataaaatt aagtcagaaa aaattatttt aattggtctt ctcgctatga
     1921 gttttggatt ctttctaatg tcaagattga aagaaagttc tgaccttatt ttatttgtaa
     1981 tttatatatc gataatatca ataggacaag ctatatttca acctgctaac aatgctttga
     2041 taatgtcaag ctgttctaga agtaagctag gagttgtagg aagcataaat tcccttgtaa
     2101 gaaatttagg acaagttata ggtattacta tttctacaac acttttatat aattttatga
     2161 gtattaaagc tggatatagg gtcaatgatt atgtgattaa taatgataaa atatttgtat
     2221 ttggaatgag aaatgtttat attattgtta ca
//
LOCUS       DAGSHT010000331         2249 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.337, whole genome shotgun sequence.
ACCESSION   DAGSHT010000331 DAGSHT010000000
VERSION     DAGSHT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.337"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            311..451
                     /locus_tag="MJB72_003611"
     CDS             311..451
                     /locus_tag="MJB72_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264669.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(448..819)
                     /locus_tag="MJB72_003612"
     CDS             complement(448..819)
                     /locus_tag="MJB72_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264670.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1368..1625
                     /locus_tag="MJB72_003613"
     CDS             1368..1625
                     /locus_tag="MJB72_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264671.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1735..>2249)
                     /locus_tag="MJB72_003614"
     CDS             complement(1735..>2249)
                     /locus_tag="MJB72_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY8264672.1"
                     /translation="IGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGF
                     LLPLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVG
                     VNNLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEF
                     LLFNWSIIPY"
BASE COUNT          931 a          239 c          258 g          821 t
ORIGIN      
        1 gtcaaataaa tataaaaaaa ttatatattt ataaaaaatt ttataataaa aatcatcctg
       61 aaaaatatat aaaaatagaa aaaacataca agattctaca ataatagatt tttttgttat
      121 tattacgagt tttttattgc aactgtagaa taaaagtatt attatataat taagataatt
      181 atctagggaa attttggata atcaaaatat attttataaa atcaaaatac aattacataa
      241 actttattaa aaatttgttg ggaatttctg actaacatta agaacgtaag tttgttgggg
      301 gatggtatat gtggaaaagt caaacactac tgttacaaaa aaagaaaaaa ttgaagatat
      361 aggaatgaaa ttagaacatt tattgagtga agatattact ttttatgaat tattacttga
      421 gattttgtat aaagaaaaag aagagaatta attctcttct ttaaggaatc tttttaaata
      481 ggcattatca atcatttttt ctatagcttc tctatcttct ttttcaagag aatatatttt
      541 ttctaagagc ttttttgttt tttcacttac tgataagttc tctatgatta atttatcttc
      601 tttgcttatt tttgtattat catttacgcc taataaataa tctgttgatg tttctaatgc
      661 ttttgctaat ctaactatta tttcactttt aggttctctt aaattatttt cataacgtga
      721 caagctagcc tcagttatac cgactttagt agcaagttct ttttgattta tatctaattc
      781 ttttcgtaga tttgctattc tatctcctaa actttccata tgtacatcac tccttaccga
      841 aattataagt tattttgata aaaagtaaaa taaaattacc aaaaatataa aaaaaaaagg
      901 ttaaaataat aaaatatatt gaaaattacc atttgtatat gtataatatg aattatacaa
      961 atggtaattt gatgagaggt ggtatgttga aataagaaac tgaattaatt aataaataca
     1021 tagaaagttt tgaaaacctt taattgagat ataaacaata tgtacaaaat tttttgcaag
     1081 taaaattacc aaatgtataa aataaatcag tagcatagat aattttatgc tttaatacat
     1141 tagattattt aatttagtta taatggtcaa aaatgcaatc tacaaataaa acaaagtatt
     1201 atcaaaaaag tattctttaa gaaaattttt tattagagat gtaattgata taaataatcg
     1261 tattaaaaaa taaaatacta agtttaaaaa tttaaataat ctataaatca attataaaat
     1321 attatttatt agaaattaaa atgaattaag taaatggagg aattgatatg ataataatta
     1381 gaagtcaaga taaaactaat ttaatgcaga taaaccaaat taaaatagat ggtagtcaag
     1441 tttatgcagt atttgaaagt aaaattgata ctgtaaaaat aggagattat gaaaataata
     1501 caagagctat acaagtgttg gacaatatac aaaacttcat tgaaaatgga acaaaatatg
     1561 attatataac atcaaataaa gtcagataca atgttaataa aatatttcag atgccagcta
     1621 aatagtgagt gtgcatttta gtttaataac taaagcccac tttcttttgt agtatgaaag
     1681 agggctttac atatttgtaa atatcgaagt acacttgttt tatcaataaa ttatttaata
     1741 aggtataatg gaccaattaa acaataaaaa ttctcctaaa acagcaagta ttaaatatat
     1801 aatagcttca tttttgtaaa gagaggtaat ttttttgctt atgtcatcat caattgtaat
     1861 agcaatttca tcttgaataa tatttttata tatagatggg aaaatatctt ttttctttat
     1921 aagattaaat aaattattta cacctaccca tataaaatat cctaaaacag cacctaatgt
     1981 gttcatcatt aaatcatcaa tatctgtagc tcttaagtta aacaattgac aaagttcaat
     2041 tgctaatgaa aaacctaatc cagttaatga aacattgata atatttctaa aatttttcca
     2101 aattaaaggt agtaaaaatc ctaaaggcat aaacataatt atatttaaaa tatatgttaa
     2161 gatgccaact gagtcaaaag gtattagatt tatttcatca attctaaaaa gcccactata
     2221 ccttcctata tcccatatgc taccaattc
//
LOCUS       DAGSHT010000332         2214 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.338, whole genome shotgun sequence.
ACCESSION   DAGSHT010000332 DAGSHT010000000
VERSION     DAGSHT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.338"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..612
                     /locus_tag="MJB72_003615"
     CDS             <1..612
                     /locus_tag="MJB72_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBY8264673.1"
                     /translation="KWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEIIRWIDIKK
                     IKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEKGNFLKPFG
                     NSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKAYLVALFIF
                     LLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            615..1454
                     /locus_tag="MJB72_003616"
     CDS             615..1454
                     /locus_tag="MJB72_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY8264674.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            1469..>2214
                     /locus_tag="MJB72_003617"
     CDS             1469..>2214
                     /locus_tag="MJB72_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBY8264675.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINV"
BASE COUNT          895 a          207 c          376 g          736 t
ORIGIN      
        1 aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac cacatatagt
       61 agaactggat ttttcactat attagcagga ttaatatttg tagaaataat aagatggata
      121 gatataaaaa aaataaaagg actaccaatg ctcctaaaga cattaccgat aatacttact
      181 ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa tagattatta
      241 gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt tctaaaacct
      301 tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag ttcttatgta
      361 tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt gatgtataaa
      421 ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt atttatattt
      481 ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt tgcagtagta
      541 ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg gaagatgaaa
      601 agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga tatgatgata
      661 tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa cttatagtag
      721 tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt gataagttag
      781 atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat gtaggcatag
      841 acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac gaaaatgata
      901 cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata tacagttgca
      961 aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga acttgtgata
     1021 tcacaagttc caacgttttg gatactataa catccataac tttttttatt gattttgaag
     1081 gtaaagacta tacaagattt gatgagaaat tgggtgttgg tggagagttt ggtgctggtg
     1141 aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa tactttggaa
     1201 atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag tatcaaaaag
     1261 attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc tatagaaaaa
     1321 attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga ggtcttatat
     1381 tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat ggatttagac
     1441 agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa taaatatgct
     1501 tttggctttt tttatatatc catttaataa gcataagttt aatggtagag atatctggct
     1561 tataggtgga cattcagggg atatatacaa tgataattca aagttttttt atgagtatat
     1621 gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca gtaaagtatt
     1681 tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc tgtattatta
     1741 caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac catacaattt
     1801 tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt taaatcatgg
     1861 gactataagt tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata ttatagataa
     1921 cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa atgttatggt
     1981 aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa gatatgacaa
     2041 tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta catggagaga
     2101 ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa atataatgaa
     2161 ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa atgt
//
LOCUS       DAGSHT010000333         2213 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.339, whole genome shotgun sequence.
ACCESSION   DAGSHT010000333 DAGSHT010000000
VERSION     DAGSHT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.339"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            156..476
                     /locus_tag="MJB72_003618"
     CDS             156..476
                     /locus_tag="MJB72_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBY8264676.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            614..1258
                     /gene="trmB"
                     /locus_tag="MJB72_003619"
     CDS             614..1258
                     /gene="trmB"
                     /locus_tag="MJB72_003619"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBY8264677.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1387..2133
                     /locus_tag="MJB72_003620"
     CDS             1387..2133
                     /locus_tag="MJB72_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264678.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
BASE COUNT          874 a          199 c          393 g          747 t
ORIGIN      
        1 tttaaggtaa taaaatatta tataaaatat tttaaagcct taggtaatat taggtagaag
       61 ttttagtaaa atatgcaaaa actaattgat tatgtacctt gtatgggtat ataatagata
      121 ttgttttaat aaaatatttg aaagggaatg atttgatgaa aattacttta ccagaaacag
      181 ctattgatac tttaaaaagt atcttaaaag acaatcaaga taaacctaat aatataagag
      241 tatactttgc aggtgttggt tgtggaggtc cttcttttgg attagcttta gatgagaaaa
      301 aagaagatga cttaacttat gaagttggag aattacaatt tgtaatgagc tctgatgaat
      361 atagtcagta tggagatata ataatagaag atacaggatt tggatttaga gttattcctg
      421 aaaatatgaa agatcaaggt ggaggaggat gctctggttg ttcagggtgc cactaagatt
      481 aagtttttag taagtttaga actctcttta tagaaataaa tttctatttt aagagagttc
      541 tttttatttt ttggtgagat aatgtataat aaaaatgtat tttactaatt ttttatatgt
      601 aaggagaatt attgtgagaa gaagacgaaa aaaaggtgca gatgaaaagc ttttaagtta
      661 tacaaaatat gttttaagag acgatataga caaattaaaa ggcaagtgga atttaaagtt
      721 tagaaatgat aatccaatac atgttgaatt tgggactgga aaaggtaagt ttataacaac
      781 tttagctaag cagaaccctg acataaatta tattgccatg gaacttaagg aagaagttct
      841 tttaaaggct gttgaaaaag cagatgcttc taatttgaat aatatactat ttttatgggg
      901 agatgtaagc aatattttgg attactttga agctaaagaa ttatcgagaa tttatataaa
      961 tttctgtgac ccatggccta aaaatagatg gagtaaaaga agactcactc attctggatt
     1021 tttagaaatg tacaataggg ttttggaaga tgatggcgaa attcacttta aaacagataa
     1081 tgaaaaatta ttcgagttta gtttaaatga aattgctgca aataattggc tattaaagaa
     1141 tatttctctt gatttaggaa atagtgagta tgaaaataat gttacaacag agtatgaaga
     1201 caaatttatg tcacaaggta tgcgaatatt tagatgtgaa gctaaaaaac gtaactagta
     1261 atatgtttat aagtttaata gtagaaattt attttaattt atattaataa agtgtacgaa
     1321 agtaggtata tataacaagg aaacagaacc ctctcataga aattaaatta ttataataaa
     1381 aaatttatgt gggagggtac tatgaattta gctaatttaa taccatttat tatttctgca
     1441 gcagctttaa gtccaataaa ttcaaatgat aaggttttga gcgaaaatca acttaaagat
     1501 atttttcctg atgcaaattt aagagcagtt gtaaaaagat atataaatcc agatgagatg
     1561 actataagca atataaaggc tttagatgga gaattttatg caactggtga gagtatttca
     1621 aatttaaaag gtatatcata tcttgaaaat gtagacaatt ttattttttg gaataataat
     1681 ataaaagagg ttcctaagga agctttaagt ctaaaagata tggattcaat aaatcttgcc
     1741 aataattatt tgattgatga cgatgtagta aattctcttt ctcataatgg tgtggatgta
     1801 aattgcgatt taaattttat tgatacaaaa gataatcagt ataaactgga ctctaaatat
     1861 cataatgttg atatagcaaa aggtgaaagt attgatttga gaaaaataat tacgaagaaa
     1921 attgatagtt attataaata ctgggaagtt actgataacc ttccaaaaga tttaaatttt
     1981 attgtatctg taagtgataa gagtgtactt tcatgtgaag atatgataat taaaggtaat
     2041 aaaacaggtc aagcagtggt aaaagttata ctaagcgaca aaaatgattt aaatacatct
     2101 caagaagttc taattactgt caatgtaaaa taatctgtca acttattcaa tcattctatt
     2161 ttcaatttaa gtgtataatc aaaataaagg ttgttagtaa cagaacgcta ata
//
LOCUS       DAGSHT010000334         2210 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.340, whole genome shotgun sequence.
ACCESSION   DAGSHT010000334 DAGSHT010000000
VERSION     DAGSHT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.340"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..868
                     /locus_tag="MJB72_003621"
     CDS             <1..868
                     /locus_tag="MJB72_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_except=(pos:608..610,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBY8264679.1"
                     /translation="VPIMTKLGLKLVRGEEIGPPEEEGYIMRGIRKNFFHELRGSERA
                     IDMLVKKMKGESFETEYPMPEFDRVTPMKPVKDLSKIKLALVTSGGIVPIDNPDKIES
                     SSATKYGIYDLTDMDSMSNKEFTTIHGGYDRAYVLENPNLVVPLDVVRELEKEGVIGE
                     LVDYFITTTGTGTSVGNSKRFGEEFSKKLLEDDVDAVILTSTUGTCTRCGATMVKEIE
                     RAGIAVVHICTVVPISLTIGANRIVPAVGIPYPLGNPMLGEEESKKIRKNIVLKALNA
                     LTEDIEEQTVFE"
     gene            1145..1876
                     /locus_tag="MJB72_003622"
     CDS             1145..1876
                     /locus_tag="MJB72_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264680.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT          861 a          215 c          363 g          771 t
ORIGIN      
        1 agttccaata atgacgaaac taggtcttaa attggttaga ggtgaggaaa taggacctcc
       61 tgaagaggaa ggatatataa tgagaggaat aagaaaaaat ttcttccatg aacttagagg
      121 gtctgaaaga gcaattgata tgttggttaa aaagatgaaa ggggaaagct ttgaaactga
      181 gtatccaatg cctgaatttg atagagttac acctatgaaa cctgttaaag atttatctaa
      241 gataaaactt gccctagtaa cttctggagg gatagttcca attgataatc cagacaaaat
      301 tgaatcttca agtgctacta aatatggaat ttacgattta acagatatgg attctatgtc
      361 aaataaagaa tttacaacta tacatggggg gtatgacaga gcatatgtcc ttgaaaatcc
      421 aaatttagtt gtaccactag atgtagttag agagcttgaa aaagaaggtg taattggaga
      481 attagtagat tattttataa ctactacagg aacaggaacg agtgttggca attctaagag
      541 atttggagaa gagttttcta agaaattgtt agaagatgat gtagatgctg taattttaac
      601 ttctacatga ggtacttgta ctcgttgtgg agcaacgatg gtaaaagaga ttgagagagc
      661 tggtatagca gttgttcata tatgtacagt agtaccaata tcacttacaa taggagctaa
      721 tagaattgtt ccagcagtag ggataccata tccacttgga aatcctatgt taggagaaga
      781 ggaaagtaaa aaaattagaa agaatatagt tttaaaagct ttaaatgcac taacagaaga
      841 tatagaagaa caaacagtat ttgaataata aaaatacaac acaacactga aataaaaaaa
      901 gatatcaaaa aataattttt taaaattatt ttacgatatc tttttttatt aggtaaattc
      961 gaaatttgca tttttttaaa ttaaatttat aaaagtgata aattagttta ttataatatt
     1021 gatacacaaa ttcaaattag atatactatg aaaagcgaga ttattacatt ttggaacaaa
     1081 tattcaacat attagatcat attaattgaa cagaaaaaat taactttata taataattag
     1141 aataatgata agaaagaagg tgtatatatt gatttcaatt gccatttgtg atgatgaatc
     1201 ctatatgcta aatcatttaa aggaaaatat agaattatac atgaagcaga agaatttatt
     1261 atataatata gagcttttta ataaagtaga agatttacta tgcaatcatg aaaagtttaa
     1321 tattgtattt ttagatatac agatggaaga gattaacgga atgcaggcag ctagaaaatt
     1381 aaggtcatat ggtaatgagt gctttataat tttcgttaca gttttaaatg aatttgtatt
     1441 tgatgctttt gaagtagatg ctataaatta tctgataaaa cctctatcta atgagaaact
     1501 atataaaact ttggataaaa tagttacaaa acttgatgat aatgaaagta attatatcac
     1561 aatacaaaaa ggttattcat attggaaatt aaaacttgct gatattttat attgtgaagt
     1621 tataaaaaga aagatttatg ttcatttaaa gaaagagact gtagattatt atgagactat
     1681 tgaaaattta gagagaagtt taccaacaaa tttttttagg tgtcatcgta gttatataat
     1741 caatttacaa tatgtatatg gatatgaaaa tggtaatgca gttatggaaa gtggagatag
     1801 aatacctgtt tctagactac gtcaacaaat attttcaaaa gttatgcttg agttcatgaa
     1861 aaataggagg atataagatg attaatttat tttcttccga tttattttta aagtacagtt
     1921 ttttcttctc ttttattgta tatagaattt taatggtaat agtgcaatac tattttctaa
     1981 tcaattttac aaattttaat tcaaaatttt ggtgttttat tttatattcg ataatttcaa
     2041 tttttttaat cattataaat acatatttta atttatataa tttattttct tcacagattc
     2101 tattttttct tatgactttt ttatttgtaa agtacataat aaaacaatct actgttttat
     2161 ctatagtatc aactttgtta gtatatttaa ttgaacaatt agtatatgga
//
LOCUS       DAGSHT010000335         2134 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.341, whole genome shotgun sequence.
ACCESSION   DAGSHT010000335 DAGSHT010000000
VERSION     DAGSHT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.341"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            93..1457
                     /locus_tag="MJB72_003623"
     CDS             93..1457
                     /locus_tag="MJB72_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBY8264681.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMISAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1596..>2134
                     /locus_tag="MJB72_003624"
     CDS             1596..>2134
                     /locus_tag="MJB72_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY8264682.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLK"
BASE COUNT          847 a          219 c          337 g          731 t
ORIGIN      
        1 atttattatg atataaaaaa taatgttagg ataaatttaa atctttttct aaaaatgtat
       61 taatataatt cttaaagaaa ggatttttaa taatggatat aaaagagtta gttaaaatgc
      121 aaagaaaata ttataataca ggtaaaacaa gagatatatc gtttcgtata gaacagttga
      181 agaaacttaa gttagttgta tcacaaaatg aagaaaaaat acttttagca ctaaagaaag
      241 atttaaataa atcggatttt gaaggtttta tgacagaagt aggcatgttt tatagtgaat
      301 taaactttgc tataaagaat ataagaaaat ggagcaaaat aaaaagagtt aaatcaagta
      361 tggttaattt tccatctata tccaaagttg ttccacaacc ttatggtgtt actctcataa
      421 tgtcaccttg gaattatcca tttcaattag cacttatacc attagtttgg tcattagcag
      481 cagggaattg tgttatatta aaaccttctg aatattctgt gtcaacatct agtgtagtta
      541 aagacatagt ggaagatact ttttcaaaag aatatgttgc agttgttcaa ggaagtcaag
      601 aagaaagtga aaagttacta ttggaaagat ttgattatat attttttaca ggaagtacta
      661 atgttggtaa aataatcatg aaatcagcat cagagcattt aactcctatt actttagaac
      721 ttggaggaaa aagtccttgt atcattttaa aagatgctaa tatagacctt actgccaaaa
      781 gacttacttg gggaaaacta ataaatgcag gacaaacctg cgtggctcca gattatgttt
      841 tagtgcatga agatagaaaa aatgagttaa tagagaaaat aaaatattat actaataagt
      901 attttggaga taatccatgt aataatgagc aatttcctaa aatcataaat cagagacatt
      961 ttaatcgaat attatctctt attgataaag ataagatagt ctatggtgga aattataata
     1021 aggagacatt aaagatagag ccaactatag tagacaatgt taattgggat gataatataa
     1081 tgaaggaaga aatatttggg cctatatttc ccattttaac atataaagat ttggatgaag
     1141 taatacaaaa aattatacaa atgccaaatc cattggcact ttatattttt acaaaaaata
     1201 agtacctaga aaataaactt ttagaaatga tttcagcagg aggatgttgt atcaatgata
     1261 cagttactca tatagctacg aattacttac cttttggtgg tattggtgaa agtggtatgg
     1321 gctcatatca tggaaaagct ggattcgata catttactca ctataagagt gtgttaaaaa
     1381 aattaaattt ggatgtccct attagatatg ctccatacga taataaatta attaaggttc
     1441 ttaaaaagat aatgtaggtt atattgtaga ataataaata taaataaaat tgagtatttg
     1501 tattctgaat atattttatt tttaatttgg tatatttgtt ataaaagtgg tgattttgtg
     1561 ataaaattta attatataga aataaggagg aaaaaatgaa aattaattgg aatacaaaat
     1621 acacaacaat agcaatttat acatttataa tagctgctag tagtattata ttttatctag
     1681 tatcatcaca aattgatgta ttttctaata atttagatgc tatatttaca acattacaac
     1741 cttttataat aggatttgct attgcatatc ttcttaattt tattttaaag ttttatgaag
     1801 acagaatttt tataaaaagt gagaaattaa aaaaactaaa acaaagttca aaaagaggct
     1861 tgggtctttt attaacatat gcaacagcta cattgatact ttacttattt atgcatttcg
     1921 tattgccaca agtaattgag agtattgttg gactagcaaa tgatatacct atgtatgtaa
     1981 acaatgcaac taagcttatt gataaattaa tgactgattt aaatcttgat gagcaatact
     2041 ttaatttagc agttgataag tggaatgagt ttgttacata tattataaag tttgtaacag
     2101 atttaatccc aatattagga agtatgttaa agaa
//
LOCUS       DAGSHT010000336         2093 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.342, whole genome shotgun sequence.
ACCESSION   DAGSHT010000336 DAGSHT010000000
VERSION     DAGSHT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.342"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            229..522
                     /locus_tag="MJB72_003625"
     CDS             229..522
                     /locus_tag="MJB72_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY8264683.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            503..1144
                     /locus_tag="MJB72_003626"
     CDS             503..1144
                     /locus_tag="MJB72_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBY8264684.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1388..1720
                     /locus_tag="MJB72_003627"
     CDS             1388..1720
                     /locus_tag="MJB72_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBY8264685.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          806 a          186 c          314 g          787 t
ORIGIN      
        1 gtttttgctt taacaattat ggtgcaatac acttttgtta agttaaaaaa attagataaa
       61 gtaagattaa ataaaagcga cttaagtaaa ttgagaatct taatatgaaa attttcaata
      121 actatttaga aatagctatt tagaaaaatt tctaaatagc tattgaaata aaatggatgt
      181 tatgttatat tctatttaga tgaaattcta aatagcgagg tgatggtgtt ggggtttcca
      241 gaaacattca aagctctttc agaccctata agaagagaaa tattggttat gttgagggag
      301 aataaaatgt cagcaggaga aattagtgaa aaatttaata tgacgaatgc tacaatttct
      361 tatcatctat ctcaactaaa aaaggcagga ctcatatttg aaacaaagta taagaacttt
      421 attttctacg aaataaatat ttcagttttc gaagaggtta tgctttggtt ttcacaattt
      481 atggagaagg gagaaaaaga taatgaaaaa agcgataaat aaacaattta ttttatctac
      541 tttaatatgt tttataccat ttattgtatc aatatatttt tataatagat taccaaatga
      601 agttgcaatt cattttgata attatggaaa tccagataat tatgctccaa aggttatagc
      661 agcttttggt gtaccattgt taatgctatg tatccatttg tatacatggt ttagacttga
      721 gaatgagcct aagaaagcta atttttcaaa tgttatacaa aatttatcaa aatggggagt
      781 tgctatactt tcagtaatta tacaaataat gatggtttta tattcaacag gagttagttt
      841 ggagtctaat ttttatacag gattaatttt aggaataata gttattttat ttggaaacta
      901 tcttcctaaa tgtaaacgaa attactcagt aggaataatt ttgccatgga ctctgaatga
      961 tgaagataat tggaataaga cacaccattt agctggatgg atttggctta ttggtggaat
     1021 attattaatt attaatgcat ttattaatat tcctttttat aatatctttg taatctttgt
     1081 aattgtaata ctaccattta tatattctta tttaatgtat aaaatttcag gtaaaaataa
     1141 ctaatataat taatcactat aataatgttt gaataagaat aatatatttc agaactgaga
     1201 tagataataa agaaattact aagatttcta ttttagttct gaaaaaatct tacttatatg
     1261 ataatttatt gagatttcaa aaaataatat aaattaggct aatgaacata aaatgaaaat
     1321 ttgtatttaa tattgaaaat ataagtacag tgtgatattc ttataagaaa aacataggag
     1381 gtagaagatg aatactagag aaatgaaaga atatagggta ttttacaata aaaaattaag
     1441 cgaaacagat gagtttttta gagtatttgg aagtcttgat gataagactt attcagaagg
     1501 ggctataagt aaaaaaaata aggaattaat gggactagct atttcaattt taagccgatg
     1561 tgatgagtgt atttgttatc atattgaggg ttgtattaat tctggagcaa gtaaagatga
     1621 aattatagaa gctataaaaa taggtgtaat tggtggcggg tctattacat atccaaacgc
     1681 aagatttgca ataaaaattt tagaagaatt taatatataa ttttgataga cgtagtttat
     1741 ttagactaat aataatagtc tatttaagtc tttgaaatta agctttattt tctaagttca
     1801 cttcttatta agtttttaga attgcatata taaatataaa tttttaaata atatagcagt
     1861 tttaaattat ttacatcata gattgtattg agaatagtat attgtcatta ttaaatcttt
     1921 tatacatttt ttattttaaa taaattaata ttaaggtgat tatatttaca tatttgtatt
     1981 tttatgaact agaatattct ttataaattc atagcagttt taatagataa ctttttcaga
     2041 tagattaaac aaagttttat ttactgtatt ctattatcag tttcctacaa att
//
LOCUS       DAGSHT010000337         2023 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.343, whole genome shotgun sequence.
ACCESSION   DAGSHT010000337 DAGSHT010000000
VERSION     DAGSHT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.343"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(110..1022)
                     /locus_tag="MJB72_003628"
                     /pseudo
     CDS             complement(110..1022)
                     /locus_tag="MJB72_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            1278..1634
                     /locus_tag="MJB72_003629"
     CDS             1278..1634
                     /locus_tag="MJB72_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264686.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1749..1925)
                     /locus_tag="MJB72_003630"
     CDS             complement(1749..1925)
                     /locus_tag="MJB72_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264687.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
BASE COUNT          725 a          265 c          267 g          766 t
ORIGIN      
        1 taaatatata ttaacaaata ataaaaaaat taatttgatt gtatataaat tagtttgtta
       61 ttaaattagt attaaaaagt tcaaaaattt acttagaagg aatctataat tagatttcta
      121 tgtttgttga gaatgcaatt acagcttttc caattatttt tacttcaact ggcttattat
      181 ctaaaataat tacttttact aacatcttgc ctttgcgttt attgcttctc cttgaactgc
      241 ttcaaatata aactctgtgt tgggtgtagg tattacttta taatgtacaa ggtacgcacc
      301 taagggtcca ttagcatttc ctgtaacagg gtcttctgaa ataccaattg caggagcaaa
      361 cattcttcca tgaacttgta gagtttcttt attatgtaat gtaaaaatat aatgtccatt
      421 acattttatt tcttgactta ttttcgataa ttcatataaa tttggagata aactatttaa
      481 tgttgataat tcctttattc caatcataac ttttgaatgt cctgtagatg atatggcaat
      541 aggacaatct gtacgaatat cttcctttaa aataccaaat gcattaagta atcttttttg
      601 ataggtttgt tgtaatggtg gagatacttc aatttttcct tgtgtcataa ttatttcata
      661 atcattatct ttttttataa tatcaactgg taatattcca gcaccagttt tttgaaatac
      721 tgttttgtta ttgaattggt tttctattgc tctaacataa tgggcagcaa tagtagcatg
      781 accacatata ggaacctcat tagttggtgt aaaaaatctg atatgtacat cataattttc
      841 ttgatgattt ttagccaagt tattgaaaat aaaagcagtt tctgaattat ttaattctct
      901 tgttattttt tgcatttgtg attcagataa gccatcagca tttgtaatta caccagctgg
      961 attacctgta aatttttctt ttgtaatgag tcaacttgat aaagacgaaa ttttttattc
     1021 atattatata atcctttcta aacattagat tagtttatct acatagaaaa cttgagataa
     1081 attattattt attattatct gtttaaataa aattaataaa ataacatatg tctatattaa
     1141 aatttaaaca tatacatcta aaaaagtagt attatgtaag gctctatact acaatcaaga
     1201 ttatattacg aattagtacg ctaaacaaga atgcactttt tagttagata cttactattt
     1261 tgaaagagag gattgagatg gatatagata ataattttga agaatttcca aatattgaag
     1321 aaagacaaaa agattttaat tgttcaattg gttttaccat gactgtgata ggaagtaaat
     1381 ggagggcaat tattttatgg catattttaa aacaagagcc aatacgttat agtcaattaa
     1441 aaaaagaagt tgttcatata agtcataaga tactgtcaca agaattaaag tatcttgaaa
     1501 gagatggact tattaagagg atttcttatc cgactattcc tccaaaagtt gaatatttgt
     1561 ctactaaacg tggtaaatca ttagaatcta tattattaga attatgtaat tggggtaaac
     1621 agtatatgag ataaactcaa ctgtttactt tatgaaaaat atataatgaa gcttatttta
     1681 attagttctt taatgaatta taaaacgtag ataaaactaa aaagggtttt ctagaaaata
     1741 aaatttcttt aattatatct gcttaccaat accatcactt gatgcaaaca agattctacc
     1801 acattctctc catattctag tataaaatca tttaattgtg tatctataaa attgcagtaa
     1861 tcgcgtgaac tgcatatata tgcagttagt atatgtgtac atctatccat tttatccata
     1921 aacatatatc atatatcccc ctaaaaaaca ttcataaaaa caaaaagaaa atatctcttt
     1981 ggcaactggc tgacataact catttcataa gtcttagtcc cta
//
LOCUS       DAGSHT010000338         2011 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.344, whole genome shotgun sequence.
ACCESSION   DAGSHT010000338 DAGSHT010000000
VERSION     DAGSHT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.344"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            45..587
                     /locus_tag="MJB72_003631"
     CDS             45..587
                     /locus_tag="MJB72_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8264688.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            915..>2011
                     /locus_tag="MJB72_003632"
     CDS             915..>2011
                     /locus_tag="MJB72_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8264689.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIG"
BASE COUNT          877 a          168 c          360 g          606 t
ORIGIN      
        1 tactattata taaaaattgg cttaaaagaa ggtgaaaaaa gtaaatgaaa atatgcataa
       61 cagtaggtca tagtatacta aaaggtggta aaagtacagg tgtaaatggt atagttgatg
      121 aatacaatta taataaaaaa ttagctccta tgttagctga gatgcttata agtcaaggta
      181 atacagtaga tgtaataatt tgtccagaaa ggcactttat gtcagaaaga gaagaatttt
      241 tctatagagt tccaaaagta aacagtggta aatacgattt attagtagag ctacatttaa
      301 ataaagctga tggaacacaa tgtggtacag aagtgcttta ctatggcact gaaggattag
      361 aatatgctaa aagagtatct aatagacttg gagaattatt tgaaaataga ggagctaaaa
      421 aaagagaaaa tttatatata ttgagaaata caaatccagt tgcaatacag attgaaagtt
      481 ttttctgtga taatgtgaat gattgtaaca aagcaaatga atcaggttat gattatatag
      541 cacgattgat tacagaaggt atattgaata aagatataaa tatgtgagat tatggtgatt
      601 taggtattga ttaatacctt ttaatattag atataaataa aaatacaatg tattagtata
      661 atttaaactt tcaaaagata gattagtttg attattgaca agagatacct ccttgaaaat
      721 attgatttat cagtatattt aaggaggtat ttaaatataa aaatttaatt aatttgctac
      781 taaactatag gtttatagag tatatatttt tatcttcagt ctaataaata atataaaaat
      841 agtagaacct tgtattataa taatataatg tagaaaaata tatagataag ataaatataa
      901 taaggtggta atatatgaaa tatacatttg aaaaatttaa gttaaatgaa aagatactta
      961 agtcattgaa gagtttagga tataatatac cttctagagt acaaagagaa gttattccaa
     1021 aattattaaa aggtcaaaac ttagtagtta ggtcaaaaac aggaagtgga aagacagcaa
     1081 gttttgcaat acctttatgc gagaatataa atgtagatta caataatatt caagctctta
     1141 tagtggttcc aacaagagaa ttagccttgc aagtaaaaga tgagatatca gatataggga
     1201 gactaaagaa ggtgagatgt agtgctatat ttggtaaaca atctataaaa gaccaaatag
     1261 ctgaattgaa acaaagggtt catattgttg tagctactcc aggcagaata cttgaccata
     1321 taaatagagg ttctattaag ttggaaaatg taaagtattt agtaatagat gaggcagata
     1381 aaatgtttaa taaaggtttt gttgaacaaa tggaaaaaat attattaaat ttacctaagg
     1441 agaaaatagt aagtcttttt tcagctacaa tagatgaaga gataaaatat atatgtgaaa
     1501 aatatatgct agattacagt gttataaata tagaagaaaa tgagagcgat actaatcaaa
     1561 aaacaagaca aatagatgac aaaataatca aggcaaatgg aagagagaag tacatccttt
     1621 taaaagaatt aatatacagc gaaaatccaa agagtgtgat aatattttgt aataccaaag
     1681 agaaagtatc taaattatat aacaagatga gtaaagaagg ttttttaata agagagttac
     1741 atgcagattt atcacaagaa agaagaattt ttgtaattaa ggattttaaa aaccaaaaat
     1801 ttaatgtatt agttagtagt gatgtagcgt ccagaggaat acatattgat gatatttctt
     1861 tagtaataaa ttatgatgtg ccacaagata aagaaaacta tatacataga ataggtagaa
     1921 ctgggagaaa aggaaatagt ggaaaggcaa ttactatagt aactgaaaag gatgaaaaat
     1981 atatagagaa tatagaaacg tatataggat a
//
LOCUS       DAGSHT010000339         1971 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.345, whole genome shotgun sequence.
ACCESSION   DAGSHT010000339 DAGSHT010000000
VERSION     DAGSHT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.345"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..903
                     /locus_tag="MJB72_003633"
     CDS             <1..903
                     /locus_tag="MJB72_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBY8264690.1"
                     /translation="IKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSFY
                     TTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTTGV
                     DKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEKIKYSKGKVKLLIEIK
                     LNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGD
                     FSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDN
                     PYLIESAIYWKKNGFISQVSDYLF"
     gene            1225..1809
                     /locus_tag="MJB72_003634"
     CDS             1225..1809
                     /locus_tag="MJB72_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY8264691.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          778 a          201 c          328 g          664 t
ORIGIN      
        1 attaaaaaag agaaaaaatc tatatcaaaa attgttttta atttagtatt aatattatta
       61 atttcttata tagagtcagt tgcattttct tcacatataa atggtatgtt taatacatca
      121 ttttcatttt atacaacagc aattgctcat agagcaggag ctgaaatggc tccagagaat
      181 actttggcag caatagaaga agcttcacat gctaaagctg attatgcaga aatagatgtt
      241 caagaaacta aagatggaga acttattgta cttcatgatt caaactttaa gagaactaca
      301 ggagtagata aaaatgtttg ggatgtaaat tataatgaag taaagacata tgatgcggga
      361 agcttttatt attatggaaa aggttattat gatgaaaagt ttgttgggga aaagatacca
      421 acactcaaag aaaagataaa atattctaaa ggtaaggtga aattattaat agaaataaaa
      481 cttaatggac atgaaaaggg agacgtagta aaaaaagttg ttcagttaat taaagaaaat
      541 aattttgaaa atcaatgtat agttgcatct atggataaaa ctatattaca aaaagtaaaa
      601 aaactggatg agaatttact gacatgctat tttatggcaa ttgcatatgg tgatttttca
      661 aagttaaatt atgttgatat atatggaatt gaatcaactt ttgttaataa aacagtagta
      721 gaaaagattc ataaaatgaa caaaaaaatc ttcgtttgga ctgttaatag tgacagttta
      781 attgagaaaa tgcttgacct aaatgtagat agtattatta cagataatcc ataccttata
      841 gagagtgcaa tttattggaa gaaaaatgga tttattagtc aagtgtcaga ttatttattt
      901 taaatattct ataattgtat tatccagtaa aagagatatt attaaaatca ataatatctc
      961 tttttaacat aaataaggtt gttaaattaa ggttattctt ataaaaattg tgcttatcag
     1021 ctataaacaa ggaataaaag tgaaaaaatt gctctgagat tattacagtt aaaagagttt
     1081 taagatgaca gagagataga ttgtcaactg tatatttaat aaatcttgtt atttattaaa
     1141 ttttatataa gtaaaatata taaattaata aatcttatgc tagaatatat atgaaaaaca
     1201 atataaattt ggaggattaa aaacatgaca gagcgagaaa aaatgttggc aggagaactt
     1261 tacgattgtg gtgatattga gttgttaact caatggcata aagcaaaaga cctagtaaga
     1321 gattataacc aaacagattc ctcggatttg gaaaaaaaag aaaaaatatt aaatgagtta
     1381 cttggtggta agggtaaaaa cttatggatt actgcaccat attatgtaga ttatggaaat
     1441 aatatttatt ttggaaataa ttgtgaagtc aatatgaatt gtacgttttt agatgataat
     1501 aaaattatca ttggggataa tgcactaatc gcaccaaacg tacaaatata tactgcattt
     1561 catccaacta atgctggtga tagatttggt aatgccaaag aagatggttc atttgaattt
     1621 tgtaaaactc aaacagctcc agttactatt ggagataatg tttggattgg cgggggtgct
     1681 attatcatgc caggtgttac tattggagat aatgtagtta taggtgcagg aagtattgtt
     1741 acaaaagata ttccaagtaa tatgattgca tacggaaatc catgccgtat tattcgagaa
     1801 aataaataaa gttaaatttt cttgcaaaat ctcaaaaaat acctttctta ctttatacat
     1861 agggtatttt ctaatacttt cacatccata ttcatttgtt aaatttttat ggaatgtagt
     1921 ttttatcatc tctatcttac attttcacta tttttttata aaagtcattc c
//
LOCUS       DAGSHT010000340         1966 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.346, whole genome shotgun sequence.
ACCESSION   DAGSHT010000340 DAGSHT010000000
VERSION     DAGSHT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.346"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..920
                     /locus_tag="MJB72_003635"
     CDS             <1..920
                     /locus_tag="MJB72_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264692.1"
                     /translation="GFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYSSN
                     INIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERAIM
                     NQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEKVT
                     SDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANAID
                     NAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIAKK
                     YQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            959..1444
                     /locus_tag="MJB72_003636"
     CDS             959..1444
                     /locus_tag="MJB72_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264693.1"
                     /translation="MYLKRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1707..1943)
                     /locus_tag="MJB72_003637"
     CDS             complement(1707..1943)
                     /locus_tag="MJB72_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264694.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTAS"
BASE COUNT          802 a          216 c          288 g          660 t
ORIGIN      
        1 ctggatttat atattggtat agttctaaaa agtttaatat taaggtcatg aactttgata
       61 aatatatagc aattttgatg attccattgt tgcttataat attatttatg caatcatttg
      121 aatatagttc aaatataaat atagatacaa gtcttgggat tgtaaagctg ctattaaata
      181 cttctataac tgaagaaatt caagcttttc ttttttctat aattggaact atatgtgtat
      241 ttttttctct atttaccttt aagaaactaa ttcaagctct cgaagatgac aaggagaggg
      301 caattatgaa tcaacagatt catgctcaaa aaaactatat tgaagaagcg aaatcacgat
      361 tatcacaaac tatatccttt agacatgatt ttaataatca tttagctatt gtcaatggat
      421 tgttgaaaaa agaccaaatt ttaaaagcac aagattattt aaataagctt gaaaaagtaa
      481 caagtgacct atcattttct tgtaatacac agaatattgt tattgatgct cttttcaaca
      541 ataaattaag tatagcaaaa caattaggca tagaggtaga ttgtgaagta attatacccc
      601 aaagcactaa catgaacgat tttgacctat gtatagtgtt tgctaatgct attgacaatg
      661 caattaaagc ttgtagagtt gtagataaac aaaataggta tttaaagctt tccactaagt
      721 tagagggtgg attttttatg atagaaataa aaaatagcta taatccaata aataattata
      781 caagtggctc aggtataggt cttttaaata taaaagaaat tgctaaaaaa tatcaagggg
      841 ctataagtat agataagaca tcagactatt ttcaaataaa tgtattgcta gttatttcat
      901 tacattgcgc tagtatttga gaacaatttc attgaaaata gtatttgtaa gctatattat
      961 gtatttgaaa agagaaagtt tgatatatag aaatgaggag gttactaaaa tgaaagttgg
     1021 aaatataaat ggtcaagcac caattacaaa tggtttagat agttgtcaaa gtgaggtaca
     1081 aggcttagaa caaaagaaaa aagcattaca ggatgaaata gagaaagtaa gaagtgataa
     1141 aagtattgat ggtgaaacgg ccaaattaaa aatagaagct ttaaaaaaag aaatggaaga
     1201 gattgaaaat aaaattcaag agttaaaagg aaataaagga aaaaaaacaa ataaagaaga
     1261 aaataacgtt tcagaagtaa ataataatct attagagaga aaaaaagaac cttctaataa
     1321 tgaaaatgta aaacaattat atgatgtttt tgaaagaagc aatgtagacg aacaatggga
     1381 taatacttat cgtgttctcc aagatgataa aaacttaaaa gtaaaattta atagacctat
     1441 atagtttatt tataaaaata gttaatattt taattatcac cactttttag aagaatacac
     1501 actttatttt aaagacccac atatttattt acagttagca catttatatg tggttttata
     1561 actattattt acaagtctat attgataact tttacatagt atatgatgtt taaatactta
     1621 ttcaaattta tctatacata aacaaaaaaa gagattttaa aatctctttt tagattgttg
     1681 acttatggct ttttgattta ataaaatcaa gaagccgttt gaattttata tgttggctga
     1741 tacccttctg atattgaaac accatttttg tttccaaaat cgtttaaaat taagaactta
     1801 ttatgaatga ctatttcatt atgaacaata agcaaataac atgaatcttc aatcaatgtt
     1861 ttaacagtag cattaaatgc ttgcttatat agttctatcg tttgtttgaa ctgcccatta
     1921 aagtaaatat gagtcaataa cataactata tctacacaaa ttaata
//
LOCUS       DAGSHT010000341         1936 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.347, whole genome shotgun sequence.
ACCESSION   DAGSHT010000341 DAGSHT010000000
VERSION     DAGSHT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.347"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            199..1704
                     /locus_tag="MJB72_003638"
     CDS             199..1704
                     /locus_tag="MJB72_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY8264695.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1818..>1936
                     /locus_tag="MJB72_003639"
     CDS             1818..>1936
                     /locus_tag="MJB72_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HBY8264696.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEA"
BASE COUNT          790 a          182 c          314 g          650 t
ORIGIN      
        1 tagaagtaat aaaaaaatag ttaatataaa agtgatacaa taataataga agtaataaaa
       61 aaaaataatt aatataaaaa tgtgtacaaa ctaatgaata ttgatatagt aagctatcat
      121 atgaaagagt atgtacacat tttttttact aatttttcac aatatgaaca taaataattt
      181 acaggaaagg aatcttttat gaaaattcaa aatactgagt ggggatatat tgaatggaaa
      241 catacttatg atgaaaataa tccaaaacaa gctatgaata tatacattgc agtaactatg
      301 cctggtaaga aacattttaa tcatgttcat tatggtcaag agcagatgat atatatttta
      361 gagggagagg gtttgtatat tataaatgga gtttggaagc cattttatca aggtatgatt
      421 ttttatatag aatctggtag cacacatgaa actataaata caggtgatag agaaataaaa
      481 gaacttattg tttcaaataa tgtagatgat gtaggagaga gtgaagttat agatattaat
      541 cctaataatt atttaaagaa gaccttgata aattatagtg aaagtacatt aaatttgtat
      601 gcagcagtag aatcaattcg tggtcaattt atagacccgt ttaaaattcc attaattata
      661 tatgatgatt catggaatat agttttaaaa aatccttatt ttccactttt ttgttttgaa
      721 aaatgtaatc ctatgaaatt tcctcaaaat tgtgattgta tgaatcaaaa aagtagcaat
      781 caatttgtat gtgaatatgg gataactata tataatatac caatactgta caaaagcaat
      841 agcataggtg ttataagagg agggtatgta ctattatctg atttgaattt agatacagag
      901 cataataatt tatatgatat tccagaggga gctgctagaa gtataaaacg cctcctgaaa
      961 caaatatcta aaaatataat aaatttttgc tcttttaatg atataagaaa ggatttacaa
     1021 gaaaaagaga agactatagc aagaacatat cattatggag agcaacttga aatgaattta
     1081 aaagtagctc aagatatggt tactaattta agaataaatc atcacttttt atttaataca
     1141 ttaaattcaa tggcaagcat agcgctagat gatggtagtt atgatttgta tagtgcaata
     1201 atagatttat caagaatgtt tagatataca atgaggtctg atttaagatt tgtagaatta
     1261 gaatctgaaa tattatacat taaaaattat ttgaatttac agaaattacg ttatggagat
     1321 gcacttaaag taaaatatct tattccagag aagttatata acctttctgt gccttttaac
     1381 tttattcaac ctattgtaga gaatgcattt acacatggat ttagagatat tgacacagaa
     1441 aagcgaattg aaataattgc aagattagat tctcagtatg ctattataga gattcataat
     1501 aatggaacta tcttagatgg aaataatata gacaaaataa aggcaggtat tcgtagtaat
     1561 aatggtcatg gtttgtcttt aatatataca aaattcacat ctgcttatgg aaataatttt
     1621 gatatggata taaagtcgag tgataatgaa ggaacttaca taatgattaa aattcctatt
     1681 gaaaattata aggaatatgt atagaacttg tgaaaaatta ataagtttta taaatgttaa
     1741 gaaatatatt agatgttttt atgttgtttt atatatctta cttttttata ttattaagct
     1801 aaaagggagg tattctcatg agaagagcac taataataga tgatgaagaa gctgttctaa
     1861 aaataataac acattttata gaaatgaaag atatgccaat aaaaattgtt ggtaaagcaa
     1921 catctggtga tgaagc
//
LOCUS       DAGSHT010000342         1926 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.348, whole genome shotgun sequence.
ACCESSION   DAGSHT010000342 DAGSHT010000000
VERSION     DAGSHT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.348"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="MJB72_003640"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="MJB72_003640"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="MJB72_003641"
     tRNA            242..316
                     /locus_tag="MJB72_003641"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="MJB72_003642"
     tRNA            321..395
                     /locus_tag="MJB72_003642"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="MJB72_003643"
     tRNA            401..476
                     /locus_tag="MJB72_003643"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="MJB72_003644"
     tRNA            482..558
                     /locus_tag="MJB72_003644"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="MJB72_003645"
     tRNA            569..643
                     /locus_tag="MJB72_003645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="MJB72_003646"
     tRNA            658..742
                     /locus_tag="MJB72_003646"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="MJB72_003647"
     tRNA            752..825
                     /locus_tag="MJB72_003647"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="MJB72_003648"
     tRNA            836..912
                     /locus_tag="MJB72_003648"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="MJB72_003649"
     tRNA            922..997
                     /locus_tag="MJB72_003649"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="MJB72_003650"
     tRNA            1009..1084
                     /locus_tag="MJB72_003650"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="MJB72_003651"
     tRNA            1087..1175
                     /locus_tag="MJB72_003651"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="MJB72_003652"
     tRNA            1193..1283
                     /locus_tag="MJB72_003652"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="MJB72_003653"
     tRNA            1292..1368
                     /locus_tag="MJB72_003653"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="MJB72_003654"
     tRNA            1454..1529
                     /locus_tag="MJB72_003654"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="MJB72_003655"
     tRNA            1590..1666
                     /locus_tag="MJB72_003655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="MJB72_003656"
     tRNA            1673..1749
                     /locus_tag="MJB72_003656"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="MJB72_003657"
     tRNA            1753..1828
                     /locus_tag="MJB72_003657"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="MJB72_003658"
     tRNA            1836..1912
                     /locus_tag="MJB72_003658"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          485 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatcaa
//
LOCUS       DAGSHT010000343         1921 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.349, whole genome shotgun sequence.
ACCESSION   DAGSHT010000343 DAGSHT010000000
VERSION     DAGSHT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.349"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            458..1120
                     /locus_tag="MJB72_003659"
     CDS             458..1120
                     /locus_tag="MJB72_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264697.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1129..>1921
                     /locus_tag="MJB72_003660"
     CDS             1129..>1921
                     /locus_tag="MJB72_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264698.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIVVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVL"
BASE COUNT          878 a          176 c          313 g          554 t
ORIGIN      
        1 tgtgaaaaaa ttaagaagat attaacatca gcagtttaga ggcaaagcat gtaaagtaaa
       61 gtctgggtaa ctaaaagtag taatttagat tgtaactatt gttataaata tgaaaatgag
      121 gaaacaggaa atataagtta ggatgtagaa aataggttac taaataaaaa gcttaattat
      181 ataatcaaag aaataggtat atactgtaca atttcatggt gaagaacttt tcctaaataa
      241 aaaggttggt aaaagagata taagaaataa aattcaatta taagatatgg tatgataact
      301 aaaggcacta tatggagtga ataggcggaa gaattttttg aaaattataa aaatagtttc
      361 tgttgctaca tatcaataag tatagataga gattgcaaaa tccatagcaa aaatcgaatt
      421 tataagtgta aataacatgt aagacaagga gtaaattatg agtattttag agataaaaaa
      481 cctaaataaa atatatggta aatcggaaac agaggtgaaa gcgttaagtc aaataaatct
      541 aaaaataaat agtggagaat ttgttgtgat agtaggtaaa agtggaagtg gtaaaagtac
      601 tttactacac ataatggcag gacttgaaac tccaacaaat ggtgatgtga ttattgataa
      661 tataaatata tcatctcttg atgaaaagaa aagatctgca ttacgtaaag aaaagttggg
      721 attaatattt caatcatata atttgattcc agttttaact gttgaagaaa atataaaact
      781 accaactata aatataaaaa ataaagatga agcttatatt aatgaattga tggagttact
      841 aggaataaaa gatagaagaa tgtatttgcc aaatcaatta tcaggtgggc aacaacaaag
      901 agtagcaata gcaagggctc ttgtaaataa accatcaatt gtatttgctg atgaacctac
      961 tggaaattta gacacaaaga cagagtcaga tgtattaagt ttattaaaag aatctcaaaa
     1021 aaaatataat cagacaataa taatgataac acataacata gatataacaa aatatgctga
     1081 tagagttata gaaatagaag atggatttat aagagagtag gtagatttat gaaacttaat
     1141 tatattgtaa aaaaatatat aatgggaaat aagaaaaata cattattaat agttgtaagt
     1201 attgtaattt caacagcgtt gtttttaatt atgaatataa taagtgaaga tgcgacaaac
     1261 ttaatgataa atcaagctaa aaatgaattt acattaaaac atgcacaata tatcaatcca
     1321 aaagacgaag aaataaaata tatagaaaat aacacaagta tagataaagt tggaaaatca
     1381 atgcttttag gaattagtga tattggtaaa ggtcaaacac tacaaattct atctcaggat
     1441 aaagttgctg aagaactaaa tgatatttac acactagaaa aaggtaaatt accagtagct
     1501 gaaaatgaaa tcgctataga ttcatggtat attaaacaaa aaaagataaa aaaccctata
     1561 ggaaaaagga taacattaga ttatagaaga caagggatag atcaagaaga tttatatact
     1621 ggggaaaaag aatttaaaat aactggtatc ttaaatagta atcctatttt aaaagctcaa
     1681 ggaacatcaa ttggccttat aagtaacgat tgtgctatca agaatattcc tactaaaaat
     1741 aaatatgatc aagttatgtt tacttttaaa aaggaaaaaa atatacagag acaatcacaa
     1801 aaacttatta aaaatggaaa tttgaatgag aataacattt attttaataa tgaattatta
     1861 atagctatat ctgatagtat gaatttaaaa ataccttata taatagttaa tatagtacta
     1921 g
//
LOCUS       DAGSHT010000344         1907 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.350, whole genome shotgun sequence.
ACCESSION   DAGSHT010000344 DAGSHT010000000
VERSION     DAGSHT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.350"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            110..1729
                     /locus_tag="MJB72_003661"
     CDS             110..1729
                     /locus_tag="MJB72_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBY8264699.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          694 a          232 c          307 g          674 t
ORIGIN      
        1 tttttattga caaaatttgt tattctatat tatggaaaca ataaatttta gttaataaag
       61 tttataattc agataaaaaa attattaatt tcttaaggga ggaaatatcg tgaatatagt
      121 aattagcctt atcggtggac ttggactttt tctttacgga atgagtttaa tgggagaagg
      181 tttacaaaaa tcagcaggag acaaattaaa aaaaattatt gaactcttaa caagtaatgt
      241 tgttatgggt gtactggtag gtacagtcgt tacaggtatt atacaaagtt caagtgctac
      301 tactgttatg gttgtaggat ttgtaaatgc aggtataatg aatctaagtc aagctattgg
      361 tgtaataatg ggggcaaata taggtactac agttactgct caattagttt catttaatct
      421 tgaaggaatt gcaccaattg ctttaggtat aggtatttta ttctatttat ttacatcaaa
      481 tcaaaaaaca aagcatttat ctgagatact tataggtttc ggtatattat ttacaggaat
      541 ggagtttatg aaagatgcag tagctccact tgctgaatat aaagcattca ctgatgcact
      601 tttatatttt agtaaaaatc ctgtattagg tatattggca ggatttgcca taactggtat
      661 tgttcaaagt tcaagtgcat ctatgggtat gttaatagca ctagcttctc aaggaatact
      721 tcctctatca tctgctttac ctattcttta tggagataat ataggtactt gtgttacttc
      781 acttttatca agtgttggtg caagtagaaa tgctcgacgt gcagctgtaa tgcatttaag
      841 ttttaatgta attggtacaa taatatttat gcttgtttta aataaaccta tatcagccat
      901 agttactcat tttgacccaa ctgatacagc tagacaaata gcaaatgcac atactttatt
      961 taatcttaca aatgttataa tattattacc attttctaaa tacatagtta aattagcaaa
     1021 tagacttata cctattaaag aaactgaatc agaaatagta aataatacta agtacttaga
     1081 tgaaaggatg tttagcactc catctatagc acttggtaat actgtacaag aagttgtaag
     1141 aatgggtcat aaagctacaa actctttgga acattcaatt gcaggatttt taaataagag
     1201 caatgaagat attaataaag cttttgagtc agaaaaagtt gtcaataagt tacagaaaga
     1261 tatattgaac tatttgttaa aactttctaa agaacctctt agagatgatg aacgctttag
     1321 aacagatttg ctttttaata ctgtaaatga cattgaaaga gtctctgacc atgcagaaaa
     1381 tatagctgaa ttggccatga gtgttaagga aatgaacatt agtttttctg atagtgcgat
     1441 aagagaaata tacgaaatat acaataagac aattactaat tttaaggatg ccttaatagt
     1501 tttagatgtt aaagattttg agttagcaaa taaagtttta gaagttgaaa atgaagtaaa
     1561 ttatttagaa aaaactttta gaaattcaca tatgattaga ctaaataatg gaagttgtac
     1621 aattgatgct ggtgtacttt atcttgactt acttactaat ctagaaagaa tttctgacca
     1681 ctcaacaaat atagtaaaac aagtactaaa acttaaacaa aaaatatagt gattattttt
     1741 atatataatc ttaatcaatg agaagtaaga agctatagat aatgcttata tcatttatgg
     1801 cttcttattt tttatgtaat actcaaattt ttatagtatt ctcttattat taaacagttt
     1861 ctgcctagaa tattccatat atatatgact tctttcataa aacttga
//
LOCUS       DAGSHT010000345         1867 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.351, whole genome shotgun sequence.
ACCESSION   DAGSHT010000345 DAGSHT010000000
VERSION     DAGSHT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.351"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            91..705
                     /locus_tag="MJB72_003662"
     CDS             91..705
                     /locus_tag="MJB72_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY8264700.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            723..1085
                     /locus_tag="MJB72_003663"
     CDS             723..1085
                     /locus_tag="MJB72_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264701.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1191..1688
                     /locus_tag="MJB72_003664"
     CDS             1191..1688
                     /locus_tag="MJB72_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBY8264702.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          707 a          211 c          322 g          627 t
ORIGIN      
        1 gacaagtctt tattttgtct aaattaaaca aactttttta caaataaata cataaaattt
       61 aaactaatct atttattgga ggagataaaa ttggaagaag taattgcaaa aattattact
      121 atagaaaatg gattcaaaga aattgaaaac attgctaaaa aaattgtaaa aaataatgac
      181 tcaaaaaagt gttattatct atcaatctac ttatataggt cagaatatta tcaagtaaga
      241 gaattggctg tgtttatact tggttttata tcagtaacta tttctgaagc tttgttattt
      301 ttaaaagata atgtgagtaa agatgaaaac tggagagtac aagaaatact agctaaatca
      361 tttgattatt attgcagtga agtaggatat gaaaaggctc ttcctgttat aaaagaatgg
      421 cttaattctg atagtccaaa cataaaaagg gctgtgacag aaggtcttcg aatttggact
      481 agtagagact attttaaatc taatcctgac gttgctatat cactactttc tagtttaaaa
      541 gaagatgata gcgaatactt gagaaagtct gtaggtaatg ctcttagaga tataagtaaa
      601 aaatatccag atttagtaaa aaaagaatta gattcatggg atatatcaaa taaaaaagtt
      661 gagtttgtat ataagcttgc ttcaaaatgt atcttggcaa agtaaaatgg aggtgaactt
      721 atttgaaact tcgtgatgag tatacttgcc cattagaact aacacatgat atgataaaag
      781 gcaaatggaa atctattata ttatggagat tacgcatagg accaacttcc ttatcaaaac
      841 ttgaaaagga aatcaatggt attaatcaaa agatgttact tgaacaactt aaagaattaa
      901 ttgaatttgg tcttgtagat aaaaagacat atgaaggtta cccacttaaa gtcgaatact
      961 cacttacacc tattcgaggg aaacaaatat tggatgcttt ggctataatg caagatatag
     1021 gaattagctt tatgttggat aatggtatgg aagatattct taaggaaaaa ggtgttttaa
     1081 agtaaataga tacccacaaa aaagtaagta atttaccatt attttcaact ggtatataat
     1141 taaattatag atatattcta ttaatttatt ataataggag gtaaattatt atgaaagtaa
     1201 ctagtacagg tatagtaaat ggtgttatag aagataaata cggtaaaaga ggaactcagt
     1261 tcaatgaagg tggtatgcct acctattcac tacctctaaa gtttgaagat gctccaaaga
     1321 atacagtatc ctttgctgta tttttagaag ataaagactc tgttcctgta tgtgggtttg
     1381 catggataca ttggttagct gtaaatataa caaaagatga gttaaatgaa aatgaaagtg
     1441 taacagctac tgattttgta caagggacta ctagttggca tggtaaacta ggaggtcttg
     1501 acagattaga ggccagtgta tatggtggaa tggctcctcc agacagacct catgtctatg
     1561 aaatacatgt atttgcactt gatactatcc ttgaccttga aaaaggattc tatatgaatg
     1621 aatttttcaa agctattgaa gggcatgttt tagatacatt tacactaaaa ggaagttatt
     1681 ctaattaatt aggttgacta tagctaatca ttaattaaat gctacattta attgatgatt
     1741 agcttaattg ttattagaat attttctgca ctttatctta atatatattt tagttgactg
     1801 gtattttaat ttatattaga ttattatcaa tttatataaa ttgattttag cttaaaactt
     1861 acttatc
//
LOCUS       DAGSHT010000346         1856 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.352, whole genome shotgun sequence.
ACCESSION   DAGSHT010000346 DAGSHT010000000
VERSION     DAGSHT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.352"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            30..200
                     /locus_tag="MJB72_003665"
     CDS             30..200
                     /locus_tag="MJB72_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264703.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            574..1671
                     /locus_tag="MJB72_003666"
     CDS             574..1671
                     /locus_tag="MJB72_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY8264704.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
BASE COUNT          767 a          174 c          306 g          609 t
ORIGIN      
        1 tgtttatttt gaattttgga ggattaatta tggattcaaa taataatact ataaaatcaa
       61 ctgttaaaaa gggtatttct tttggttctt gtttagcaat gattatttct tatactgcat
      121 ggaaatctat tccatgggct atttttcatg gcttaatgag ttggatatat gtactttatt
      181 attgggttaa gtatgcatag attaaattaa taaataagta tgtaatattg tgaagtttaa
      241 gaaacaaaac gtaagtgagt gtctcgaagt gaacttttta gtttataagg cactcttttt
      301 tgtattaaat aaaatatatt ttcattactt aaacaatgaa aaagatgagt atctctattt
      361 taatacatac tcttatattc attcatttat caacaagagt tttaaaaaga aagctatcta
      421 aaatctattg taatctaagt gcttctcttt aaagtcttaa ctttagtaat gatatataag
      481 tttataaggt tcttatttca ataatgcaaa ttttacttag aagttcagac tttggtatga
      541 agaaatgggt aataataaaa aagaggtgtt gatatgaaag aaaaattaga agaaaaatct
      601 aagattatta aaacatcaaa agagtataaa cgaactataa ttgaagaaaa ggacagagaa
      661 tggaccaaaa aattaaatga catacttagt agaggtgatt tttctaatga aaaattagat
      721 aaaatcaagg atttaattcc aaaagaaata ttgactagtg aaaatgttgg agaagttgta
      781 actgaagatg ggtatgctaa gcctgagtat gatgaggtat ttaaatcatt gtttacttta
      841 aataatgatt acgatttact tgcaaatttt ataaatgata tcctcaaaga tgctccatat
      901 gctaatagga atatcaaaca gttcactcaa ataaaaagaa taattaaggt tgaaaccgac
      961 ccaacaatta attacattgg tgagaaaaga cctagacttg atattttggc tgaagatgaa
     1021 gaacgtaacc atataaatat tgagatgcaa agagctcttg aagaggatta tttggaaaga
     1081 gctgaatatt atcttagtcg tgtacatggt agaaagttag aagaaggtaa agaatataaa
     1141 gaaattggaa aaactgttgg aatacatata ctaaatcatg taaaatataa tcacatagaa
     1201 gattatgtaa attgcctgag attaacaatg gacgggcatc cagatatttt ttctagtaaa
     1261 actgcattat attttataga acttccaaaa attcgtaaat ccagttgtat agcaaacaga
     1321 gttctaatat ggggtaaact tattgataat ccttctcata tagatattag aatactatct
     1381 aaaacggatt atgttattaa aagagcactt gatagattaa aagaattagg tagtaatgag
     1441 gactatttat taaatttaaa gagaggtgcg tatattatga ataaaagtag aaattttaaa
     1501 gaagaaattt ttcaagaagg tattgaaaaa ggtattgcaa aagggaaaag agaagagaaa
     1561 tttaatatca tcataaaatt aaaaaataaa ggatacaatt tgagtgaaat atgcgatatt
     1621 atagatgatt tgaataaatc agaggttgaa aaagtctata atcaaaacta atactatgat
     1681 tgagatggca ctagataaat acaaagcttc ctcttttaaa tttaaattaa tataaaactt
     1741 tgttatttaa atatggtaga tataaagaga tatataaata ataggtacaa agaggtgtat
     1801 aaataaagga gataattcaa aataaaagtt tgaaccatct ccttcttata gaaaaa
//
LOCUS       DAGSHT010000347         1838 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.353, whole genome shotgun sequence.
ACCESSION   DAGSHT010000347 DAGSHT010000000
VERSION     DAGSHT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.353"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(282..1610)
                     /locus_tag="MJB72_003667"
     CDS             complement(282..1610)
                     /locus_tag="MJB72_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY8264705.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          672 a          329 c          209 g          628 t
ORIGIN      
        1 aaaagctatg tataatattt gttgtacaga taccacttag tttgataaaa aacactttaa
       61 aataaagtat tttaaaataa ataatttttt agatgtttaa ttttataatc aaaaaactaa
      121 aaataaaatg aataccttaa aataaacttt ttagttcatg gaacgctctt tttttgtaaa
      181 attaaacata tctttaccat tttaacaata aaaaagagac ttatctctat ttgagacagt
      241 ctcttttatt ttaattatga agcatttgta aactacttgg tttatctttt tactcctcta
      301 gtcatataag catacactgg taagcctata gctgttatta caagacctcc tatagatatc
      361 atagtagttt tcatacctgc aaaacacaat tgattaacta caacaaatat accacttgct
      421 atagctatta taggaacaat aggatataat ggaactttat actctcttgg tatatttgga
      481 tgagtctttc taagtttcat aacccctata aaagttaata catagaatat ccaagtagca
      541 aatacagcta agtcagttaa taaattaaat tgacctgaca aagcatatat agatgcaata
      601 acagatacta atgctattgc atttgctgga acttgagcag aatttaattt agaaaatata
      661 ccatactttg gtaaagtctt atctgtagct aaagtatatg ccacccttga accagttagt
      721 aaaaatccat ttaatgctcc aaacactgaa attaatatac ctacagatat tatttttcca
      781 cccattgaac caaatataac ttcagcaact gctgatgcag gagaagcata atttgctaat
      841 tcattagctg gtaataccca aagatacgct aaatttatta taaaataaac agccataaca
      901 actgacaatc cacctattat agcttttgga aggtcttttc caggattctt catttctcca
      961 gctaaagtac caacattcat ccatccatca aaagcaaata atatagcaat taaaacttga
     1021 cctagtacac tacccaggct taatccttca ccaaccatag gtgtaagtat tggattattt
     1081 ccaccacctt taataaatcc aacaaccatt attaaaataa gtggtattaa cttacaaatt
     1141 gtagaaacag tttgtatgac tcctccagtt tttgaaccta acatattaag ccctgcaact
     1201 attattataa ttccaattgc tattggaagt aaaagtgatg gactaccaat tagcgctgat
     1261 gcttgttctc caaatataac acctaatgct gccatcatcc ctggataaaa taatacaatt
     1321 tgcatccatc cagttaaaaa acctaatttt tctccataaa tttcttttat atataccatc
     1381 attccaccag tttttggtat cgcaactgat acctctgcag ctgtaagacc agctgttata
     1441 gttatgattc cagctattaa ccatgccaat ataccaagac ctggagctcc tccagttaat
     1501 gtatagactg cttgtggttt aaaaaatacg cccgctccta taacggaacc aacaacagtt
     1561 gataatgctg ctgatacgcc aagagtcttt ttcagttcat tacttcccat ttttttcctc
     1621 ctcattaatc ctatttaatt ttaatttcat agttaaataa gcatatatta tgccaatata
     1681 tagaaaaata gctttattat ttattaaaat aaaataaact ctacttagct ttagtaatct
     1741 tttattttta gttatgttta aatataaaaa aattataaaa accaaaaatc caaatataat
     1801 aatacttcta agtaaatgta ttttttatat tgtataat
//
LOCUS       DAGSHT010000348         1834 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.354, whole genome shotgun sequence.
ACCESSION   DAGSHT010000348 DAGSHT010000000
VERSION     DAGSHT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.354"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1431
                     /locus_tag="MJB72_003668"
     CDS             <1..1431
                     /locus_tag="MJB72_003668"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBY8264706.1"
                     /translation="AIIKGISNDGGLYVPSEFPNVKNELINLVNLTYSQIAFFVLSKF
                     LCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELYHGPTLAFKDMALTIMPH
                     LLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKIIVFFPEDGVSPVQKLQM
                     KTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNNYILSSANSINIGRLLPQ
                     VAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYAKQMGLPINKLICASNDN
                     NVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEISNRNTDIVNKLLSDLSD
                     KGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYLIDTHTAVAYNCYEKYKK
                     ETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIIDRLSSISKIDIPKPIKNL
                     KNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1441..>1834
                     /locus_tag="MJB72_003669"
     CDS             1441..>1834
                     /locus_tag="MJB72_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBY8264707.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVA"
BASE COUNT          725 a          211 c          285 g          613 t
ORIGIN      
        1 gcaataataa aaggaatatc aaatgatggt ggtctttatg ttccaagtga atttcccaat
       61 gtaaaaaatg aacttataaa tttagtaaat ttaacgtatt ctcagatagc attttttgta
      121 cttagtaaat ttttatgtga ttttactgaa gatgaaatta aaaattgtat agaaaatgca
      181 tatgatgaaa agtttgattg tagtagtata gctccattaa ataaagtaaa tgatacttat
      241 ttcttagaac tatatcacgg tccaacttta gcatttaaag atatggcact tactataatg
      301 cctcatcttc ttaaaacatc tataaagaaa gataatttag agaaagatgt tgtgattctt
      361 actgctacat ctggagatac aggtaaagct gcacttgagg gatttaaaga tatagataaa
      421 ataaagataa ttgtattctt cccagaagat ggtgtaagcc cagtacaaaa acttcaaatg
      481 aaaactcaaa ctggaaaaaa tacatatgta gttggtataa aaggaaattt tgatgatgca
      541 caatcaggtg taaagaagat tttttctgat aaagaattta atcaaaaact attggataat
      601 aattatatat tatcttctgc aaattcaatt aatataggaa gacttcttcc acaagttgca
      661 tactattttt attcatatat gaaattagtt agcaatagtg atataaattt aaatgataaa
      721 ataaattttg ttgtcccaac tggtaatttt ggtaatattc tagctggata ttatgccaaa
      781 caaatgggtc ttccaataaa caaattaata tgtgcatcaa atgataataa tgttctttat
      841 gatttcttta aaactggtgt atatgataaa aatagaacat tgaaattaac tacttctcct
      901 tctatggata tattaatttc aagtaattta gaacgattac tgtttgaaat atcaaataga
      961 aatactgata ttgtaaataa gcttttatca gaccttagtg ataaaggagt ctacaaaata
     1021 agtgatgata tggaaaaaaa tttagcctca ttctatggag aatattcaaa tgaagaagaa
     1081 gttaaaagca cgataaacaa tgtgtttaaa aattataatt atttgataga tactcatact
     1141 gctgttgcat acaattgtta tgaaaaatat aaaaaagaaa cttctgataa cacaaaaact
     1201 gttattgtaa gtactgcaag tccattcaag ttttctgaag atgttctaaa gtctatagat
     1261 tgtgatttta aagaccttga tgactttgct ataattgata gattatcaag tatttctaaa
     1321 atagatattc caaaacctat taaaaatctt aaaaatgcag aaatactaca taaagatatt
     1381 tatgaaaaag atgaattaaa actagctata aaaaaattct taaaggtata ggtgattatt
     1441 atgttagaga ttatagttcc tgcaacaagt gctaatatag gtccaggatt tgattgtctt
     1501 ggaattgcgt taaatatata caataaattt tatgttgaag aaattgaaag tggtcttgaa
     1561 atagaaggtt gtgaagatgc ttacaaaaac gaaaacaact tagtttatac atctatgaag
     1621 tacttttttg atagagttaa accagaaaaa atacctgctg gtatcaaaat aaaaatccaa
     1681 agtgaagtac ctatatgtag aggtcttggc agtagtgcct cttgtatagt agctggagtt
     1741 atagctgcaa atgctctatc aggtgctaat ttagataaaa atcaattact aaatatagct
     1801 tcagaaatag aaggtcaccc agataatgtt gctc
//
LOCUS       DAGSHT010000349         1825 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.355, whole genome shotgun sequence.
ACCESSION   DAGSHT010000349 DAGSHT010000000
VERSION     DAGSHT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.355"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(91..858)
                     /locus_tag="MJB72_003670"
     CDS             complement(91..858)
                     /locus_tag="MJB72_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264708.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            1146..1820
                     /locus_tag="MJB72_003671"
     CDS             1146..1820
                     /locus_tag="MJB72_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264709.1"
                     /translation="MFKIMVVEDDISLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
BASE COUNT          673 a          242 c          222 g          688 t
ORIGIN      
        1 aaactaaaaa atacaaatac actaaataca attgaaataa agtacattaa ataattaaaa
       61 aaactattct ttacattttg cattgcaatt ctaaataaag tcatttttat taactcctcc
      121 aattaaggaa actgtattta ctattttatt atagaactct ctgttacttt ctccctttac
      181 aatttctgtg aatattattc catcctttat aaagagtatt ctattgcaaa aactcgcact
      241 tatagcatca tgagttacca tcaatattgt tttattgctg tctttgttta attttaatag
      301 acaattcaac aaatccctag cagatttaga atctaatgct cctgtaggct catctgcaag
      361 tactaatgat ggttttgttg ctatagctct acatacagct cctctttgct tttgtccacc
      421 agaaacttcg ccagggtatt tatctaatat agattctata tttaattctt ttgaaatctc
      481 ttttattcta ttttctatta ctgatactct ttcatttttt aaagaaagag gcaacattat
      541 attttctttt attgtaagtg tatccaaaag attagaatct tgaaatacaa agcctagatt
      601 atcacgtcta aaatctgcca attcattttg cttcatttta atagtattct ttccatttat
      661 ataaatatct cctgatgaag gtaaatctat tgtagaaata atatttaaaa gtgttgattt
      721 accagcaccg gaagacccca taataccaac aaattctcct ttataaacct tgaaagttat
      781 tcctcttaga gcttcatgtt ttgaaccttt tcttccatac tcctttttta tattttcaac
      841 agataatatt tcttccatag ttaataatca cctttcatca caattagtta tttatcaatt
      901 ctcacaaata tattatatat attaaaatgg cttttaaaca tttaattata ttaatttaag
      961 gttacatttt cgtaagtatt aattatattt cttcaaattt attgcaatta tgtttaaaat
     1021 ataatttatc aagtaaaata tagttatatt aatataatta ttttaggtaa taaggtagaa
     1081 atattttaat ttatcaagta gaatatagtt atgttaatat aactattttg aattaggagg
     1141 taagaatgtt taaaataatg gtagttgaag atgacatctc attgaaaaat atcatagcaa
     1201 agtgtcttac taaatgggga catgatgttc atcaaataga aaatcttgaa aatataatag
     1261 aagagttcaa aaactataac ccagaattag ttttattgga cataaacctt ccattttatg
     1321 atggttttca ttggtgtaat gaaataagaa aaatttcaaa agttcctata atttttattt
     1381 cttcaagaaa ttctaatatg gatgtaataa tgggtgtaaa tttaggtgca gatgattata
     1441 tacaaaaacc tttttctgta gatgtcttag tagctaaagt taatgccctt ttaagaagaa
     1501 cttataattt tgttgataat aactcaaatc agattattca caatggtgtt actttagatt
     1561 tatcaactgc aacaataaac tatgaagata atactattga gctaacaaaa aatgaaatca
     1621 aaattctcca tgagcttatg aaatataagg ggcagatagt aagtaggaat aaactcatga
     1681 aaaaattatg ggataatgat tggtttgtag atgataatac attaactgtc aatgtaaata
     1741 gaatacgctc aaaattaaat gaaattggtt tagaagattt tatagaaaca aaaagaggat
     1801 tgggttatat aatcagttaa aacaa
//
LOCUS       DAGSHT010000350         1807 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.356, whole genome shotgun sequence.
ACCESSION   DAGSHT010000350 DAGSHT010000000
VERSION     DAGSHT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.356"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(87..653)
                     /locus_tag="MJB72_003672"
     CDS             complement(87..653)
                     /locus_tag="MJB72_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBY8264710.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            814..1467
                     /locus_tag="MJB72_003673"
     CDS             814..1467
                     /locus_tag="MJB72_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBY8264711.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
BASE COUNT          614 a          220 c          224 g          749 t
ORIGIN      
        1 agactgaaaa ttatatttta tacaataaaa gtttatttaa ataacattgt attttaatat
       61 ttattttcag tcttacttta ttaaaattat tttttggtta tgtctggaaa atatctgtct
      121 actaattttt ctgaaatatt ttttaattgg tcagccattg aagtcagact taataaggct
      181 atcaataaat catctttatt ttctggattt atactaatat tttcaaaatt agactctata
      241 aagtccggta tattctttct ttgattttct tttattaaca tgaatttttc gtaacatgaa
      301 actaaatctt tttcactgaa attatccttt tcatacacac catgtaagat tcttttaatt
      361 tcttgtattg taagtgtatt tttcatagaa taaataatta tcatctgtaa aatttgttct
      421 ttagtatact tttttccctt aactggtttg attaatccct ctttgctgta attatttatc
      481 atggttttag taagaatttt atcactttca aatcttttat tagcagaaag cttattgtca
      541 attagagaaa taatttgatc catatataag tcaatagatg gaatatcgtt tgaagttata
      601 tctgcattat ttagtgtttc tagtattata gtattaagtt cattattcat catatgttac
      661 ctccataata tatataatac actaaattag aaagtaaaac aatattaagg cttgtaaaaa
      721 aaacataaag tggtataatt aaatatataa tatagtaatg gaaactatat ttatgaatct
      781 gatttttaaa atacataatt gtaggaggac gaaatgtatc aatttttttt aaagggtaga
      841 gacccaataa gcagtttaac tcattttata ggtgcttgtt tatctctttt agcaactata
      901 attttagttt ttcaatctgt tacattacag gaaacttctc tcttgatgat agtatctgta
      961 tctgtatttg gactttcact aattgcttta tatagtgcaa gttcgtatta tcatttttta
     1021 aaggggacac cagaacaaga attgtttttt agaaaagtag accatgcaat gatttacgta
     1081 ttgattgctg gctcttatac accaatatgt ctgaatttta tggaaaaaaa agaaggcata
     1141 atatttgtta cagcaatatg gatagttgca tttattggta taataataaa aatattttgg
     1201 atggatgcac ctagatggtt atctaccagt atatatttac ttatggggtg ggcaattgtt
     1261 tttgatataa atgcattcaa ttcaattcct aaggattgcc ttagactatt aattatggaa
     1321 ggtgtatctt actctatagg tgcgattatt tatataatta aaaagcctaa tataagtcct
     1381 gagtttggat ttcatgaaat attccatatt tttattatga taggttcttt atttcacttt
     1441 ttagcagttt tattgtatgt tttatagttt taaaagattt gattaattga tactaaaatt
     1501 ctacaatgaa taaaatggaa ctgccttata tagattatat cttcgacaat aagacagttc
     1561 cttattataa atattaaatt ctacttataa taaaattttg ataaaaatag tccattcaaa
     1621 atgcctattc cataattcct ttaatgtact tttcatttac gacatatagt ttgaatttat
     1681 gtactataat tatatcctct aaattctctg aaacactaac aattctgaca catttgtttc
     1741 taatttctac aggtaaatct aaatcatttg ttaaattcac atagtcatca tatttataca
     1801 taaaatc
//
LOCUS       DAGSHT010000351         1773 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.357, whole genome shotgun sequence.
ACCESSION   DAGSHT010000351 DAGSHT010000000
VERSION     DAGSHT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.357"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1373)
                     /locus_tag="MJB72_003674"
     CDS             complement(<1..1373)
                     /locus_tag="MJB72_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY8264712.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIA"
BASE COUNT          564 a          320 c          157 g          732 t
ORIGIN      
        1 attgcaatat atactcctat accctttaaa ctaataaatt tatcatttag taaatttata
       61 tctggtatcc aacaattctc tgttaattcc aaaattaaaa attcaggtct taattgatac
      121 tctactaaac attctactat aaaatctcta aaaaaatctt cctttaactg aatatacgat
      181 acatttacag atattttaaa gtctggattt attttatgcc attctttaca ctgttttaca
      241 gcttctttta taatccactt cccaactggt ataattaaat tactctgttc tagtattggt
      301 ataaattcaa caggagatac ttctccataa gtactcgaat gccatcttaa aagtacctct
      361 gcccctatta cctcttttgt aacagcattt acctggggtt gaaaaaacag ctcaaagtca
      421 ttaaaattat tttcaacgca ctctcttagt ttttgttgca ttgaaattac ctttagttta
      481 ttttcataga gctcttgtga aaaaaactta attctatttt taccactaat ttttgctata
      541 tccaatgcaa tatcagcatg tttaaataaa tctaaataat tatctccatc ttctggatac
      601 atagcaacgc ctgcagtcac tgtgcaatag tacttatttg attcaatttc atgctgagta
      661 tttgtataca tctgtatctt ttcatatatt ttctctatag tttctttagt acacataggg
      721 taaaaaaatg caaattcatc tccatctaac ctatacaatc ttacatcttt tggaagatat
      781 tctaatactt cagtagcaat tgcacgcaat actttgtcac cataagaatg tccatatttt
      841 tcatttatat ttttaaaatt atcaatatcc ataataaaca tagcaccatt tttataaatt
      901 ttctctttta taagataatt cattctatgt tcaaattgtt ctctattcca cgttcctgtg
      961 atagaatcaa atttattttt ttcaccaata ttctttattc tcccgactag aaatatagtt
     1021 tttgggtcat ccgacacata aactttacct ctacaagaaa tccatactat ttctttatat
     1081 ttattaatca acctatattc taaattgtgc tcaccttttt ttccatgtag taattcttgt
     1141 atatcatctt tccatatttg aacatcatcc ggataaacaa ttttactcca acaattgacc
     1201 aaatcagatt ctatctcttt acttaagtta aattcatcaa aaattgctga agaaatttta
     1261 aatttattcc tatttatatc ccaaaaaaat atataatctt ctgtactttg agataaaagt
     1321 tcaaagaaaa tttctatatg gtctaaactt aaagagttcg attcttctct catcttttcc
     1381 ccctacaata ttactattag tgtagtttaa tcaacaaaat atccttattt agattatact
     1441 attttactaa ttttttgtct catcaaacac atttccccaa ctttagttat cttatcaaat
     1501 ctcttactct taactaagtt ttttgagatt cataaagtaa tttttctata atcattactg
     1561 gtgatttatt tttacctgct aaaacaccat gttaaatcat ttattaaatc attttctctt
     1621 aaaattctat ttttcgttat ataaagagtt atcagttctt tattctataa aacttttcta
     1681 ttactcttga atttatttta tttttttctt cgcctattct ctttggattt ggtattgctt
     1741 ttctatagat tttggcaaag aagtgtctct tcc
//
LOCUS       DAGSHT010000352         1760 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.358, whole genome shotgun sequence.
ACCESSION   DAGSHT010000352 DAGSHT010000000
VERSION     DAGSHT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.358"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            376..843
                     /locus_tag="MJB72_003675"
     CDS             376..843
                     /locus_tag="MJB72_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBY8264713.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            854..1069
                     /locus_tag="MJB72_003676"
     CDS             854..1069
                     /locus_tag="MJB72_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264714.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            1510..1683
                     /locus_tag="MJB72_003677"
     CDS             1510..1683
                     /locus_tag="MJB72_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBY8264715.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
BASE COUNT          668 a          192 c          198 g          702 t
ORIGIN      
        1 accaatttac ataatctttt taatataaac aatgatacaa tatatgggac agcacatatg
       61 tatatagcta cttatattac atattacagt tggcatattc gtatttgtaa tgcttatacc
      121 aattttaaaa tacaccatca gtactaatgg tattaaaata atctataata aagttaaaat
      181 tattccaatt agtacaatat tatttaatat tttacaactc atattctttt ttatataaat
      241 ttaccacctt atacattctt atttttagta tatattataa tggttttata tattatatca
      301 ataatttatt attgaaattc aataaattat atattataat caataatata ctttatataa
      361 aggagatgat attttatgat tacaaaattt tttagtaata tattatcagt atttctatat
      421 tttctattat cacttttagg agttgctggt ataatattct tatgtatgat acctatgttt
      481 ttatatgatt ttagttctga caaagtaatc gctttaattg caagaatact atatttttct
      541 gactacttta tagtcatctt attcttgata tttataatta atagtactaa gtattcccca
      601 ttcgtacttg aaaatgtaaa aaggtttaag gtaatgggat gttgtttatt gataaacact
      661 ataattgaat gtataaatgg ttataattta aatactaata atataagaat ttttggaact
      721 gatagtggcg gtatctcacc acttatggtt atctctttta tatttgcact aatgtgcttt
      781 gtaattgcag aaacatttga taaagctata aaaataaaag aagataatga tttaacaata
      841 tagaggtgat gttatggcaa tagtagttaa cctagatgta atgatggcaa aaaggaaaat
      901 atcacttaaa gatttggcag aaaaaataga tattacaaat gcaaatttat caattttaaa
      961 aactggaaaa gcaaaagcta ttagatttac cactctaaac gaaatttgca aagctttaga
     1021 ctgtcagcca ggtgatatac ttgaatatgt agaagatgaa aatgattaaa aaagagtgtt
     1081 tagaaacact cttttttatt atactatatg atttttattc ttttactata ttattgacat
     1141 taatattaac tttgtttaaa gtcataacaa aatatgttct aagaaagaaa ctgttccttt
     1201 atctatttgt tatccattac ctataagaac tgtatcacac taaatcatac ttttaaattg
     1261 ataattttac cacagattcc tatatacatt cttttctacc taatatttgt ttaaactgtt
     1321 agcaaaacat ggaacttttt catttactta tacctccatt tatattattg catagtttat
     1381 agctacgtgt tataaattac attattgatg attttatcta acatttgtaa tcttttagat
     1441 actctttgaa gttacaacac tgttatactc taattaagta aactaaatct gatacaagtt
     1501 ggtgattata tgaaaagaaa actttcaacc tattttaaag gatatatttt aggtttatgt
     1561 atagcatttt tattatttac ttttatatta aaaaaattaa ttatagaaaa tatcattgga
     1621 ataataattg gaactggaat tgcattttta ctgatttacc tatttaataa aaaaagaaaa
     1681 taatattcta taaaaaggtc ttttagtgaa tttacaaaaa attaataaga ataatatata
     1741 cttagtattt aaaaagggta
//
LOCUS       DAGSHT010000353         1759 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.359, whole genome shotgun sequence.
ACCESSION   DAGSHT010000353 DAGSHT010000000
VERSION     DAGSHT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.359"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            87..824
                     /gene="cas6"
                     /locus_tag="MJB72_003678"
     CDS             87..824
                     /gene="cas6"
                     /locus_tag="MJB72_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBY8264716.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            830..>1759
                     /locus_tag="MJB72_003679"
     CDS             830..>1759
                     /locus_tag="MJB72_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBY8264717.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQA"
BASE COUNT          759 a          119 c          311 g          570 t
ORIGIN      
        1 atttactgat tttttcaaat aatttatact tggaccaata tacttcccgg tctagtgtta
       61 atatattaat tgagagaggt gatattatga aaatgaaaat tgaatttagt acaggttgta
      121 taccaatatc ttataattcg ctctttatga gtattataaa agaagcaata agaaaatcta
      181 atgaagatta ctataaaaac atgtactatt ataaagaaaa gaataataaa aagactaaaa
      241 attttacatt ttcggtttat ataaaaaagt atagtattga aggtgacaat tttattatag
      301 aagataaagt aatattaaat ataagtactc cagatttaga attgggtttt catatataca
      361 atgggttaat gacttctaaa aaatgtttat ataaagacta tgaacttaca agaattagaa
      421 tcgatttatc aagagaaaag aaggtaacag aagaaagggt actatttaat gcattgtctc
      481 caatctgtgt aaagtctaaa gaaggaaaat tcttggagat aactgatgat agatacatag
      541 aagaactaaa ctatataact aatgaagtgg ttaaaaatta tagggggaat ggattaaaaa
      601 gaaagttaga atttgaaaat ataggtctca aaaaggtagt agttaaagag agtttaagag
      661 aatttaaaaa aataacaggt aaagagtatc aatatattaa tggttacaaa ggtaaattta
      721 tattgaaagg tgatattgat gatttaaatc ttatctacaa tctaggaatt ggttttagac
      781 gtgctcaggg atttggagat gtagatattt tagaatggag gtgagataaa tgaagttaag
      841 agtttataga ggagattggt tttttaatat gggtatagtt ggatttttaa atataataaa
      901 gaaagcagat aaacaagctg aaatatttat tatggaagat tatatcgaat ttgatagctt
      961 atttcttgaa aactttcatg agtattattt taattacttt atggatgaat atgatgtatc
     1021 aaaaagaatt aaaaaaaata ttgattatag tataaatttt attaaatcta agcctgatag
     1081 aatcaaagat ggaattaaaa aaattaaaga tagtgtgaaa caacaaaatg ataagattaa
     1141 aaaatttgat gaagaaaaat ttaacctgat aaaagagaag ctagatagta tgtctaagat
     1201 aaaaagttat gatgaatttg acagtttaga agctttagta gatgagacta tagatatatt
     1261 taaaataaaa tcaataaatg acaggcttac agcaaatctt tataaatatg tagttcaaga
     1321 taattatttt ggacaagtta gtttttttaa tgttgctaag gcgaaattag atttagatgg
     1381 attaaaacag gtaatgttta atgattattt aagacaaatt atatattttg gagagttaga
     1441 agatttgtta gaagagaatg actatgataa gttgaaaaac tatttaaatg atagattaaa
     1501 tagtatagct aaggatataa atgaaaaaaa agttagtaag tctagtataa atactataga
     1561 aaaaattatg aaggaaataa ataaaaactt tataaagaaa aataaaagca tagaagatat
     1621 aaaaaagtat atggatagtc tagaagtatg tgaaatgtgt ggtttatata agggaatatt
     1681 agatgaatac tcagaaagta attttgctcc acttggagta agcaccaata atgctaggaa
     1741 tatgttttgg aatcaggcg
//
LOCUS       DAGSHT010000354         1749 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.360, whole genome shotgun sequence.
ACCESSION   DAGSHT010000354 DAGSHT010000000
VERSION     DAGSHT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.360"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..535
                     /locus_tag="MJB72_003680"
     CDS             <1..535
                     /locus_tag="MJB72_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBY8264718.1"
                     /translation="IDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNLPVIHIQH
                     ISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEKKNITNLV
                     ICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQVFMASLQN
                     SFADVKNTDKFLSNYYK"
     gene            755..1429
                     /locus_tag="MJB72_003681"
     CDS             755..1429
                     /locus_tag="MJB72_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBY8264719.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
BASE COUNT          668 a          221 c          249 g          611 t
ORIGIN      
        1 gattgatatt caaaatgatt attttaaaaa tggaaaatgt gaattatttc aatcagaaga
       61 aactgctgaa aatgctaaaa aaatactact gttttttaga aaaaataatt taccagttat
      121 tcatatacag catatatcaa cagataaaac agcttctttt tttcttccta atactcatgg
      181 ttcagaaata aataaaattg tttttccact agataatgaa gaaattataa ttaaacacac
      241 accagatagt ttctttgaaa cagatttgca aagtatcctt gagaaaaaaa atattactaa
      301 tctagtaatc tgtggaatga tgagccatat gtgtattgat acaagtgtaa gaacagcaaa
      361 gaaacttcga tacgacataa ctctaataag cgatgcatgt acaacgaaga atttatcttg
      421 gaataatata gaaattaatg caaatactgt acatcaagta ttcatggcat cactacagaa
      481 cagttttgca gatgtaaaaa acacagataa atttttatct aactattaca aataaaaaca
      541 tacactatgt taaaagagca agtttgattt atttaagctt gctctttaat catttaaaat
      601 tagatgcttt ctatcatttt tatatacata atatacgttt atacatttta tattcatata
      661 taactattat ataaatttta aactttacta tattcacata aaaaatgtag tattatagta
      721 tatataatat tatatagaag ggggtgctag ataaatgaac catcatatat tgattcaatt
      781 tgaatatata gcaagaatca ttactgctgg tttctgtggg gctttaattg gatatgagag
      841 aaagaataga ctaaaagaag ctggaatacg cactcatttt atagtggcat tagcttcagc
      901 acttataatc attgtttcca agtatggatt taatgatata gtgggtactc caggtattgg
      961 tctagaccca gcccgcgttg cagcacaagt tgtcagtggt ataggttttt taggtgctgg
     1021 attaatattt attagaaatc aatcagtaag tggtcttact acagctgctg gtatatgggc
     1081 aacagccggt acaggtcttg ccattggctc tggattatac ttagttggta ttgtttcaag
     1141 tatcattatt gttattgtac agataacact tcatagagat aggaaatgga tgaaactccc
     1201 tacagcagag caagtttctc ttaaaatttc taacagcact gattccatag aatatattga
     1261 aggtaaattt aaaaaatcta atattgaaat tataaacatg aatattaaac gccttgatgg
     1321 ggattggtta gaagtagtta tatatactaa gttacctaaa ggatataaaa aaacaaattt
     1381 attaaaatta tttagcgata atcctcttat agaaacttta gaaatataat ataaaaattg
     1441 aagcttaaaa ataataatat atttaatcaa ggtcaatata taaataatta agggatgagt
     1501 ctatataaaa ttcatccctt gttttttgcc attcatatat attttttcaa gatatttttg
     1561 tttatctatt atctagccaa gtaacccttg tgaactaaac tttttaatac aaaaacttca
     1621 atacaaaact aaatataatt tttattaaac attctataca tatagtttgt ttcttgagtg
     1681 aaaaatagat ttcatattgt taagatattg ttaaatattt caattttctg acaagtatgt
     1741 tgaacaata
//
LOCUS       DAGSHT010000355         1748 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.361, whole genome shotgun sequence.
ACCESSION   DAGSHT010000355 DAGSHT010000000
VERSION     DAGSHT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.361"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            313..1605
                     /gene="brnQ"
                     /locus_tag="MJB72_003682"
     CDS             313..1605
                     /gene="brnQ"
                     /locus_tag="MJB72_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY8264720.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          638 a          215 c          298 g          597 t
ORIGIN      
        1 atacaactta ataataaatc gaataatcta aaaaatctta atattttaat tattcgtaat
       61 agttaatttt atcaagaaaa attagagaaa aaacaaaaaa ccaactatgt ttaaagtata
      121 caggttgatt ttgtttaatt aagaaagttg tattaaatat tgtgaagatt ttataaatta
      181 tgaaacataa ttgacaaatt taagtcaaaa atgtataatt aaataaaatc tcgtcaaaag
      241 gttttcattt aattaagaga ttggacttat tttttagtaa aaggtagtaa aattcaaatg
      301 ggggtgcttt aaatgggtga gaaacaaatc aattttaaag atgtaattgt aattggattt
      361 gcactatttg caatgttttt tggtgcaggg aacttaatct tccctccata tctaggagtt
      421 ttatctggaa gtagttggct agtagctttc ataggattcc tttttgcaga tggtggtttg
      481 gcattattag cagttatagc agcaactaag ttcaatggag acacatcgaa gatgttctca
      541 agagcaggca aaggattaag tattgcatta ggatgtgcaa tggtaatctg tatagggcca
      601 cttttagcaa tacctagaac agcagcaaca acttatgaaa tgggtatatt acctacaata
      661 ggttctggaa taagcccagt aatattctct atagtattct ttgctctagt attggtttta
      721 acaataagac catcaaaagt tgtagatata gttgggtcaa tattaactcc agctttactt
      781 atagctttag cagtattaat agttaaaggt atcgtatctc cattaggaga aataagagac
      841 agttctttaa tacaaaatgt atttgcagaa ggtataactc aaggttatca aacaatggat
      901 gcattggcag ctagtgtatt tgcatcaatt ataatcatgt cagtaatagc taaaggttat
      961 acaggtgaga aagaaaagat gaaagccaca ataagtgcag gtgtaattgc agtaatagga
     1021 atggcattag tttatggtgg attatgttat cttggagcaa ctgtgtctca acaatatgga
     1081 caagatgttc aacaaacagc tttaatagtt gcaataacag cagctttact tggaactaca
     1141 ggtaaaattt tattggctat tatagttgca ttagcatgtt taacaacagc tataggtctt
     1201 tcttcagcag ctggtcaata tttctcaaca cttacagatg gaaaattaaa gtatgagcat
     1261 atagttatag tagtttgtgt atttagtgca atcatatcaa actttggagt aagtacaata
     1321 atacaattct cttctcctat actaagtatg gtatatccag caactataac tctagttata
     1381 ctagcattat ttagtaataa aattaaaaat gacaacgtgt tcaaatgtgc tgcatacatg
     1441 gcattattag taagtgtatt aacagttgca acaagttttg gtataaaaat tcctctagtt
     1501 aatagcttac catttgcttc attaggattt aactggatag ttccagttgt aatagcagga
     1561 ataataggaa actttattcc atctaaaagc agtaaaactg cataataata aaaactaatt
     1621 gataattatg gaattgcctt taaattatat aggcaattcc tttttatatt taaaagaatt
     1681 attaaattat tgtagataaa agaacattat taatcctatt tgataaattt aaaaatttat
     1741 ttttggag
//
LOCUS       DAGSHT010000356         1740 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.362, whole genome shotgun sequence.
ACCESSION   DAGSHT010000356 DAGSHT010000000
VERSION     DAGSHT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.362"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            72..1115
                     /gene="holA"
                     /locus_tag="MJB72_003683"
     CDS             72..1115
                     /gene="holA"
                     /locus_tag="MJB72_003683"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBY8264721.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1302..1568)
                     /gene="rpsT"
                     /locus_tag="MJB72_003684"
     CDS             complement(1302..1568)
                     /gene="rpsT"
                     /locus_tag="MJB72_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBY8264722.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT          683 a          171 c          268 g          618 t
ORIGIN      
        1 tgttatttat tacataggag tgttttttta catattttac atagaaggaa aagagataaa
       61 ggagcaagag aatgaattac aaggatatta taaggaatgt aaaagagaaa aactttgaaa
      121 aaatgtatct tttttatgga agagagtatt atttaattga gaatgcaata aaagcattta
      181 aagatagtct aaacgaggga atgcttgatt ttaatttaga tataatagat gggaaggaaa
      241 tagtcttaaa tcagcttata agctctatag aaacacttcc atttatggat gataggaaga
      301 tagtaataat aaaagacttt gaactattaa aaggaaagaa aaagaatttt acagatagtg
      361 atgagaaata cttaatagag catttagaca atattccaga caccacaact atagtttttg
      421 ttgtatatgg agatgtagac aaaagaaaat cattagttaa gaaaatagga aacaatggaa
      481 ttgtttttga ttgtgataag ctttctgata tggatttgtt taaatggata aaaaaatctt
      541 ttgctttaaa tgatgttata atagataatt ctcaaatcat gtattttatt gaacaagaag
      601 gatacagaga taagagtagt gaaaagacat tatctgattt agaaaatgaa ataaataaaa
      661 taagttcatt tgttggtaaa gggaataatg taaccaatga tgtaattgat aaattatctc
      721 aaaaaaaagt tgagaatgat atattcaaat taatcgatta tattggagaa caaaatgctt
      781 ctaatgcaat gaaaatatta aatgatatga tacaagaagg cgaatctgta ttaggtatat
      841 tttctatgat agcaagacaa tttaaaataa ttatgcaagt aagacagtta caacttgatg
      901 gatattcaac aaaacttata gctgataaat taaaaatgca tcaatttgtt gtaggaaaag
      961 cattaaagca aactaaaaat ttttctgatg atataattgt ggaaatatta aactatatat
     1021 tagaaagtga ttataaaata aagacaggtc ttataagaga tactttagca gttgaaatgt
     1081 tggttagtag atactgtaaa agagaagcaa tttaaaaaat ctatatatta ataaaaaata
     1141 aatagcaaga tatgttatag ttcagagtag atgtaagcta aattaagttt gatatacaat
     1201 caactataat atgtctaact atttattttt tattgtataa atttacaaaa taaaaaaccc
     1261 ctacataggg gttattatat ataagaaata taaactggta attaagcgtt cataccgttt
     1321 aatttttgag ctaatttagc tacttttcta gctgcagcat tcttatgtat agttccttta
     1381 gaagctactt ggtatattct tttttgagcg tattggaact tagcaacagc atcctctctg
     1441 tttccagctg caacagcatc ttcgaatctt cttattgcag tttttatttg agattttcta
     1501 actctattta aagcagtttt cttttcgata acacttattc ttttcttagc tgattttata
     1561 tttgccaccg tgttcacctc cctgtatttt tttatcatat tcatcgtcaa catattagat
     1621 tttaacagaa ataaatctaa aaatcaagga ttattattta tatattttaa taatgttttt
     1681 tattttgatt ttttagactg taaattgtgt ataaataatt tgcaatatct tgtttttaaa
//
LOCUS       DAGSHT010000357         1728 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.363, whole genome shotgun sequence.
ACCESSION   DAGSHT010000357 DAGSHT010000000
VERSION     DAGSHT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.363"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            368..1606
                     /locus_tag="MJB72_003685"
     CDS             368..1606
                     /locus_tag="MJB72_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBY8264723.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          753 a          145 c          283 g          547 t
ORIGIN      
        1 aaatttacta cttattaatt taaatacatt gtatattcta taaaaaaatt tgttaaatac
       61 tggagatata ttgagaatac aaagaaaatg gtcgaaaata tttgttggct aaaaaataga
      121 taaaatgata caattgcttg tataaagggg aaaaataaac aaataaacta tataaataaa
      181 gaaatagcaa ttaatatgac ataatatatt tataaaatta agaatataaa tagatatttt
      241 aaggtattag ttgttaaatt aggaaatatt aaaaaaaata tatatttata tttaaactat
      301 tttgttattt atataaaaat acttatctag ttactagcaa ttattagtag aaatggggga
      361 aatagagatg aataagggat tttacaaatt aaattcagtg gtattgaact ttgatattaa
      421 gaatgatgtt aacaagataa gatttaatca ttatataatg agaaaagaaa acatacaaag
      481 acttcaagga actctgccaa acggacaatt ttatatgaca gtgagaagag ctgctacaga
      541 tttacaactt tctatttcta ctataagtag gcttgtaaaa gagtttttgg atttagggat
      601 aataagatta gtgtctaagg gtgtaaaagg taatcgttct gtatatagtt acgtaagtat
      661 agagtttaat ggaagtttat ttgatgatag taaatatatg gaacttgact gtgtatgtaa
      721 tgatgaattt attgataata gtggcataga gtttgattgt gatagcaaag ataattcttt
      781 taatggagat ataaatgtag aaactgtgca ttgcattaat gagtatttgt ctgatgatga
      841 tgtagagcat aattataaca atttccataa taaagatact ataaaggata aagccatttc
      901 taatattagc actatcaaaa aatatattga tgaatctttt gataaaaata aatcagaaaa
      961 ttctaactta caacaggagt caataaattt caactttaat gatacatgca atgaaataga
     1021 taaaagaacg aataatagta tagaaaataa tcaaaataaa aggcctatga gattgagtga
     1081 catcagtgtt ttaagtgata aaggtatgac aaataaaaag aattacttta atcataatca
     1141 tggtatacaa aaagggacta aatctgtaac aaagaaaaaa gaatccttaa ataaatttaa
     1201 taaaaaaaat atacagcaaa gcattataaa tatgctaaat aaaaaatcag gtaagaattt
     1261 taaaatcgat tctccaataa gcataaaact tatcaatgaa aggctaaagg aaggctatgt
     1321 cttagaagat ttctacaaag ttattgaggt aaaagtggct aagtggaaaa ataccataat
     1381 ggagatgtat ataagaccag aaaccttgtt tagctataag tttgaatcct atgtaaatga
     1441 aaacatatct atgaaaagtc aaagtaatgt tcacccatgt aaagaaggca atagtaatga
     1501 tagcagtaat tatatacatt ctgattatac aacatatgat ggcagtgtgc gtgagagaaa
     1561 aaaatatgag aatgtaagat actggaatcc tatatgtgag atttaattag tttaagaatg
     1621 tatttatgat aaataagata ttaatagaga atagtgagct atagcaaatg atatatcaat
     1681 agttggtagt aagttatatt aagtaagata ttactatcag gtagtgaa
//
LOCUS       DAGSHT010000358         1707 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.364, whole genome shotgun sequence.
ACCESSION   DAGSHT010000358 DAGSHT010000000
VERSION     DAGSHT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.364"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            62..1000
                     /locus_tag="MJB72_003686"
     CDS             62..1000
                     /locus_tag="MJB72_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBY8264724.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1384..1608)
                     /gene="secG"
                     /locus_tag="MJB72_003687"
     CDS             complement(1384..1608)
                     /gene="secG"
                     /locus_tag="MJB72_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBY8264725.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          589 a          244 c          211 g          663 t
ORIGIN      
        1 tgaaaaaaac taatggaaat ttaattgaat tatcaaatca tagaatcacg gagagtaaac
       61 cttggaaaat ttaatcctat caataaatgt tgttcttcct ttgtttctca caatgtcact
      121 tggatatgtt ctaaaaaaat taggtctatt tgatactcat actttaaata aaatgaataa
      181 tgtctgcttc aagtctttct tgccattatt attatttttt aatatataca atacagattt
      241 agatggttct cttaatttta ggcttatggc tgtagctgtt ataattattt tttcaatctt
      301 cttaatctta gtctttatta ttcctaaaat tgaaaaagat aataagacaa ggggtgcatt
      361 aatacaaggt gtatttcgta gcaatttcgt tatatttggt attcctgtga caatttcact
      421 atttggcgat aagagtattg gagtcacatc tttattaatt gcagttattg tacctatgtt
      481 taatgtatta tctgttgtaa ttcttgaaat ttttagaggt ggcaaagtaa atataaaaca
      541 tatgattaaa agcgtcttaa caaatccact cataatcgcc tcttttatag gattgacaat
      601 gctaatttta aatattaata tacctacatt tttacacaaa actattggtg atatttctaa
      661 aatagctact cctttatcac ttataattct aggtggctct tttagttttt ctgctataaa
      721 ggggcatata aaccccatta tattgggcgt atgtggaaaa cttatacttg tacctgctat
      781 atttttacca attagcattt ttcttggatt tagaaatgta gaattagctt cacttatgat
      841 aatgttatct gctccaactg cagtttcttc atttacaatg gcacaacaga tggattctga
      901 cagtgaatta gctggacaaa ttgtagtatt tacttctgga ttttctataa ttacagtatt
      961 tttaataata tttattttaa agcagaataa tcttatttaa tgatatttga taacacaaat
     1021 tggtattatg agaaacatat ataaaatcga tttaattgcg taaaaacata ttattatact
     1081 atttttacta tataatataa ctttgtgtat attatctcat taaacatggt gaaagtagtg
     1141 ttgctatata ttttttagtt tgaaaaaaat atataaatac aaacaatata taaatacaaa
     1201 taataaacgc caacaaatag tcaatctata aaataaaaac tggactaatc atataaaatc
     1261 attagtccag cttttatttt acgtactttt tatgttgatg tacttttatt aaaaactaaa
     1321 tttaattttt aaatgtctag caaatgcact aattaaataa tggccagtta ttcaacaata
     1381 tcactacttc ttaactataa gtaatgccaa tgcatttata aaaaataata ctgctgttat
     1441 ctttgtaact ctagcaagaa atgcttgttt tccttttggt ttaaagtaag cttgacttcc
     1501 ttctccacca aattctgaag gtaaagcatt cttagaatct tgaggcatta tgctaattac
     1561 tagtaaaatc cctagtacta cttgcacacc cattaaaatg ttgctcattc ttgcccctcc
     1621 tatctgttta ttacttaaat ttcattactc tttagtgtat cacaataata tagccatttc
     1681 aagactatta tgctataaat aacatac
//
LOCUS       DAGSHT010000359         1683 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.365, whole genome shotgun sequence.
ACCESSION   DAGSHT010000359 DAGSHT010000000
VERSION     DAGSHT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.365"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            64..1203
                     /locus_tag="MJB72_003688"
     CDS             64..1203
                     /locus_tag="MJB72_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY8264726.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1229..1417
                     /locus_tag="MJB72_003689"
     CDS             1229..1417
                     /locus_tag="MJB72_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264727.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          718 a          138 c          245 g          582 t
ORIGIN      
        1 taatcaaaaa tagtgtaaaa attaatactt tttgttaata ttaatagata aagataatct
       61 aaggtggtga ttaaactgac aatagaagaa tacaaaaaca aatactttac tggaaataaa
      121 atcgtacttt ctataaatag ttttgataat aaagaactat actatcatga aactgaaaat
      181 atattaaatt taataaatga aatagataaa ttaacatcaa gtaagatatt taaaacaata
      241 gaagataaaa atgatttggc actagagtct tattattcta gtgtaataga aggtgctttt
      301 tcaactagaa agattgcaaa atctataatt agaggaaaga tgaaaccaag taataaaagt
      361 gaatatatga tttacaataa tcatagagcc ttagaatatg gattagacaa cttagataaa
      421 ttatatagtc acaaatttat atatgatttg catcatatat tgggcgaaaa ctgcttggat
      481 tcagaagaat atgagtatag aactgaaaaa gtatatgtat gtgattcaaa aggtgagatt
      541 atacatatag ggcttgaacc tttgaagata tatgacttta tgagtaaatt aatagatttt
      601 atggaaaata gcaaagtaag taatttaatt aaaagtgcta taattcattt ttactttgta
      661 tatgttcatc cttttagtga tggtaatgga agaacatcaa gagccttatc atatctatat
      721 ttgattgata aaggatatga tactttcaag gagttttcaa tatcatatat gatatctaaa
      781 aacagaacta agtattataa ggctatactt gatgtagaaa acaaaggaaa taatttaact
      841 gtatttatag agtttatgct caagtctata atacaatcta ttaatgagat gcgtaatatg
      901 catgatagaa aatctttaga aagtatatta aaagaagaat tgtttgaaaa tgacataact
      961 ctatcagcaa cagaagaaca tatattaaaa tatatttgca ataaagataa ctattctatg
     1021 acattagaaa attatataaa aaagaataaa agtagatact taaaggctgg aataaaggaa
     1081 attgaattag ttgaccagtt gatggaagtg tttaataatc ttgaagagat agagattttg
     1141 tctaaagaaa aaaatatata taaagttaat gataagtact taaaaatgct tgatatagac
     1201 taatttagat aatctggaga gggaatatat gaataataag aaaaaggcat ttttgatttc
     1261 tggggctata aacgtaattt cattaattat tgttttttca ttgtttcatt ataatattat
     1321 agaaaatagt acacaaaata tgataataac tgtaatctta atttttattt ttgaaatgat
     1381 aaaaataaaa atcatagaga aatactataa actataaaca agaaatatct tttctaagag
     1441 catattgtac aatatgctct taatttagtt atatagattt aatataaata atgtattttt
     1501 tgtagatgat ttatgtaaga tttatgacca atttgtgtat gatttgtgtt ctttaatcat
     1561 gacaatatta tattgtaaaa aataaattta ataaaaaatg acttggaatt tatattctag
     1621 gtctttttta tataaaaatt taataagttt caaatgttgg taactttgac agtagaaaac
     1681 gtc
//
LOCUS       DAGSHT010000360         1677 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.366, whole genome shotgun sequence.
ACCESSION   DAGSHT010000360 DAGSHT010000000
VERSION     DAGSHT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.366"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1211
                     /locus_tag="MJB72_003690"
     CDS             <1..1211
                     /locus_tag="MJB72_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY8264728.1"
                     /translation="CEKIINNNQVILSNKVNGRWVKIPIECYEVLKYSVDNALQLGNL
                     YDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSPTNRCNLSCDYCC
                     VDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLELLDYIKEKFKGK
                     IILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVFCKVLNVIRLIKL
                     KKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGNENYSKYLNDKLD
                     IDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDLKIFNILEFNENI
                     LERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLKNNKKMFDANCKK
                     MKNILEPILW"
     gene            1259..>1677
                     /locus_tag="MJB72_003691"
     CDS             1259..>1677
                     /locus_tag="MJB72_003691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264729.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPT"
BASE COUNT          707 a          133 c          286 g          551 t
ORIGIN      
        1 tatgtgaaaa aataataaat aataatcaag ttattcttag taataaagta aatggaagat
       61 gggttaaaat tccaatagaa tgttatgaag ttctaaagta ttcagtagat aatgcattac
      121 aacttggtaa tttgtatgat gtattccaag aaaatgaaga taaaaaatat tatgaacgag
      181 ttatagataa gttagagcat ataggacttc taagtgattc tattctaagt gaatttagac
      241 tagagagaat aaacaaaatt tcattttctc caactaatag atgtaatcta agttgtgatt
      301 attgctgtgt agattctaaa atagataata acgattattt agatacatct caagtaagaa
      361 aagtaataga taatatagtt aaattaaatc caaggatatt aaccattaca ggtggagagc
      421 cattgatgag aaatgatttc ttagagctgt tggattatat aaaagaaaaa tttaaaggta
      481 aaataattct gtctacaaat gcaactttaa ttaaagaaaa acagattgat gatataatta
      541 atggtttata tgcaatagaa attagtttag atggttatga tgaattaagt tgtagtgaag
      601 taagaggtaa aggagtattc tgtaaagtat taaatgtgat tagacttata aaactaaaaa
      661 aattcgagaa cgtaggtttg tctatggtgg taggaaagaa taatgagaaa gacatagaga
      721 aattcaataa attaaatgaa gatttaggta caacgcctat aataagaaat tttatgaata
      781 ttggtagagg taatgagaac tatagcaaat atcttaatga taaacttgat attgattaca
      841 tttgcaaaga tgattatgtg gatgaaagta gtttatgttc aaatatgtgt aaagcaggtg
      901 ttaatcagat aagtatagat cataaaggag atgtatatcc gtgtccaaac ttagagtata
      961 aggatttaaa gatattcaat atattagaat ttaatgaaaa catattagag aggattctta
     1021 atagagaaat ggatatattt aatatatttg ataaattaaa accagtaaat attgagagtt
     1081 gtaaagaatg taaagtaaat ttattttgta caacttgtcc atcgaaaata tatactttaa
     1141 aaaataacaa aaaaatgttt gatgcaaatt gtaaaaaaat gaaaaacatc ttggaaccta
     1201 tattatggta aggattagtt ttatattcaa ggtaatttat tttagaggtg attattaatt
     1261 gactaaagta agaatagcag tcttagacac aggattagat gtaaatgatg atgatattaa
     1321 tggaaattat gtttatgatt caaattttca attgaaagat ttggggggga aggaaataaa
     1381 tgacataaat ggacatggaa cttttgtatc gaaaaccata ttaaatataa gtaaaaatac
     1441 ccaaatttat cctattaata tatttaatga ttctggaaaa acaagttctt taaatcttct
     1501 tagtacactc agcaagttga ctatgtcgga tatacagata ataaacattt cagctgcatc
     1561 tattaatagt atgtatgaaa aagaattaga aaaggtttgt aatattttaa atagtcaagg
     1621 tagatttatt atttgttcaa aacataataa atatgaagat aaagaaagta ttcctac
//
LOCUS       DAGSHT010000361         1654 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.367, whole genome shotgun sequence.
ACCESSION   DAGSHT010000361 DAGSHT010000000
VERSION     DAGSHT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.367"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1573)
                     /locus_tag="MJB72_003692"
     CDS             complement(<1..1573)
                     /locus_tag="MJB72_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBY8264730.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGE"
BASE COUNT          487 a          275 c          163 g          729 t
ORIGIN      
        1 tttcaccttc atattctttg tcatcttcat catacttaaa ttttactatt ttcccaccag
       61 gcactttatc tagcattatt gatttagctt tttcagcact aataatagaa ctgttgtcat
      121 tagtattaga gttattctta tcattatcat caaagtcatc atcagaatca ttgtccatgt
      181 caatatctac aattcttcca tcaaatgcac ttatctctaa ttcatatgtt ttcttatttt
      241 ttattacttt agcctcatat tcgttatctt cagcatcata tttacaatag attaaattac
      301 cattaggaac agcatctaat gctatagact tagctttatc caatgatata ttttgtgtac
      361 ctaaattatt atcatttaag tcatcatctt tttcaatttc tattatttta ccagagtagg
      421 catcaatttc tatctcatat aggatattat cctttataac tttgcagtca tattcattat
      481 catcttcatc ataataacaa actaaaatat tgcttcctgg aagagcttgt tttaatattt
      541 ctttagcctg tattaaagat atttttttag ttttatttct tatgttcaca ttttttagtt
      601 ttaaaatatc ttcaaatgct ttttgattat atcctcctcc tacttgtgtt atttcatcta
      661 tatctttatt tttaataaat tctttttgac tatttacaag gctaccatta gatatcatta
      721 taggagaatt ttctttagca gctagtactc ctattgataa tgaatctact aatgcatttt
      781 cttggttaat accatcttta gctacaaata cattatttat ttttttatct ttataaaatt
      841 tatcaataat ttttgcatta gtattgtttc tatctgaacc actgattctt tctctgtttg
      901 gatatttctt tactgtattg tctgataaaa tcttatttcc tccaactatg taagattttt
      961 ttatagacct attgtttaag aaatttatac tactatcatt gctgttttca tctgatacta
     1021 atattatagg catattatgt attgctgctg gagaagctat actaactgca tcagatagtc
     1081 cctttagtcc atttacaact gcaacatttt ctaccttttt tatcttatct atttctttaa
     1141 gtacatttgt agacatttca tatatattgt ttcctactat tttatttatt tttatgttaa
     1201 gtgattttat ttcattttct actttagaag aaatgctatt tccactatct acaatgtaca
     1261 cattatctac accaagcctt ttaagctcag ccttagttga attttcaata ctgttgttgt
     1321 ttactaaaag tataggtgca ttctttagct tagcaaatgg atttgcacaa agcgcacttt
     1381 gaatagaatt accattaact ataatggctg tttttgattg aacccatccc aatttactta
     1441 cttctactgc tgtttcatat cttgttttac ctattaatct ttcttgcttt gtagtattgt
     1501 ctgcaaaaat tgctgaagaa ctagaaaaaa acataatacc tgatattaat atagctaaat
     1561 ttttcttatt catattttta cctcacaatc tttaaaaatt tgttatttag catacttaat
     1621 aaaatataat cttaaaatca aattgatgct gagt
//
LOCUS       DAGSHT010000362         1650 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.368, whole genome shotgun sequence.
ACCESSION   DAGSHT010000362 DAGSHT010000000
VERSION     DAGSHT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.368"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            286..1557
                     /gene="serS"
                     /locus_tag="MJB72_003693"
     CDS             286..1557
                     /gene="serS"
                     /locus_tag="MJB72_003693"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBY8264731.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          693 a          170 c          298 g          489 t
ORIGIN      
        1 taactatata agattgataa agaaaaagtc aaaatagata attgattagc aaaatagata
       61 taattataat gaagttaaat ctagcaatat tctcctaaaa ttaaatttca tttatttaat
      121 taaatataat ttgacaatgg gcaaaaaagt gagtaaaata taaataaaat aaaaataaca
      181 ggttcggata aagaaaaagg tttcttaacc tcaagcataa agtatattat ctaagttatt
      241 tgagattaag agataaatcc aatcttaagt actgaggagg aaaaaatgct tgatataaaa
      301 agaataagag aaaatttaga ttgtataaaa aaagcaatgg aaagaagggg cgaaaaagac
      361 tttaaccttg atgaagtagt taagttagat gatgaaagaa gaaagattct tcaagaagtt
      421 gaagttatga aaaatgaatt aaatacagca tctaaaaata taccaaatct tataaaagaa
      481 ggtaaagatg tagaaaatga aaagattaaa ctaaaagagt tatctgataa aattaaggta
      541 atagaccaaa atcttaaaga agtagaagat aagatggaat acctattaat gagaatacca
      601 aatgtaccac atccagaagt accacaaggt gaaactgatg aagataatgt tgaagtaaga
      661 acttggggaa agactactac ctttgatttt gaatctaagg ctcattggga aattggaaca
      721 gaattaggta tacttgattt tgaaacagct agtaaaataa ctggttcaag atttactctt
      781 tacaaaggtt taggtgctag attagagaga gcattattaa atttttactt agacacgaat
      841 acaaaagtaa atggatatac agaagttatt ccaccattta tggcaaatag aaatagtttt
      901 ttaggtacag gacagttacc taagtttgaa gaagatatgt ttaaaataga aggtctggac
      961 tattttatga ttccaacatc agaagttcct ttaactaata tacatgctaa tgaaatactt
     1021 aaatttgaac aattaccgat aaattataca gcttatactc catgttttag gtcagaagct
     1081 ggttctgctg gaagagatac aagaggttta gtaagacagc atcagtttaa caaagtagaa
     1141 atggttaaaa tagttgcacc agaagaatct tacaatgagt tagaaaagct tactaacaat
     1201 gcggaaacta tgctacaatt attgaatctg ccttataggg tagtaaagat atgtacaggt
     1261 gatttaggat ttacggcttc ttttaaatat gatgtagaag tttggatgcc aagttacaat
     1321 aggtatgttg agatttcatc ttgttcaaac tgcgaagatt tccaagctag aagagcagga
     1381 ataagattta aaagagataa agattctaaa gctgaatatg tacacacatt aaatggctct
     1441 gggttagcta ttggaagaag tgttgcagct atattagaaa attatcaaca agaagatggt
     1501 tctgtggttg ttcctgaagt tttaagacca tatatgggag tttctgttat taaataagag
     1561 ttaaattatg tatttttaag caatacaata atatagaggt aataaaaaat aattaatata
     1621 aaagtgatac ttctaatata gaagtaataa
//
LOCUS       DAGSHT010000363         1640 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.369, whole genome shotgun sequence.
ACCESSION   DAGSHT010000363 DAGSHT010000000
VERSION     DAGSHT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.369"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            125..436
                     /locus_tag="MJB72_003694"
     CDS             125..436
                     /locus_tag="MJB72_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY8264732.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIARPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            484..801
                     /locus_tag="MJB72_003695"
     CDS             484..801
                     /locus_tag="MJB72_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY8264733.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            1014..>1640
                     /locus_tag="MJB72_003696"
     CDS             1014..>1640
                     /locus_tag="MJB72_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8264734.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDEN"
BASE COUNT          717 a          140 c          271 g          512 t
ORIGIN      
        1 cacttaataa tattaaaata acaaaaaagt attgaaagta tattgcattt aaggtagaat
       61 taaataaaat attataacat tataatgttt atatagttgt taataaaaat ttggaggaaa
      121 atatatgata aaaataatgt tggcctgttc agcagggatg tctacaagtt tattagttac
      181 taaaatggaa agtgcagcta aagaaaatgg tatagagtcg caaatttggg caataccaga
      241 atcaactata caaaatgaaa tagaaaaatg tgatgtatta ttactaggac cacaagttag
      301 atatgtatta cctaaggctc aagaaattgc taggccatat aatataccag tagaagttat
      361 aaatatgatg cactatggaa cagttaatgg agaagctgtt ttaaatagag cgatagaact
      421 taacaacact aagtaatata aattataatg tatttttaat atataaattg ggagaatcaa
      481 aggatggatg aaaagattat agaaatatct tttaatataa taggttatgc aggtgaaggt
      541 aaaggattag cttttgaagc aataaaagag gctaagaatg gaaatataga aaaagcaaaa
      601 gaattattaa aagagtctag ggaagttata aataaagcac atagatatca aacagaatta
      661 atacaaaacg aagctagtgg aaacaggact gaaataagtg tcatacttat acatgcacag
      721 gaccacttaa tgaatggcat gaattttcaa cagttagctg aggaaatcgt tgatttacat
      781 ttgaaattac aagataaata aaaattaaag ataataaatt aagagagttc ttaaaagata
      841 cattatgtgt agcacactaa ttgttagatt attgattcaa tatttagtga gtaatacatt
      901 aggattattt tgaggactct ttttatattt agtttaaagt taataaatat ttattattaa
      961 taatataaga ttatatgtta taatattata acatataatt taagaggtga atcatgttaa
     1021 gaaatcaacc attatatata caagttgtag atatattaaa gcaaaaaata ttaaataacg
     1081 aatataaaat tgatgaaaat attccaacag aaagggagtt agagcaagag tttggagtca
     1141 gtaaaattac tattagaaaa gctattgaga tattagaatc agaagggtat gtagagaaaa
     1201 aaagtggaaa agggacaaaa gtaataagta atagtctttt taataagcta tcaaaagcag
     1261 agtctttttc aagtatactt ttatctaaag gtcataagtt atctaaaaag actatttcaa
     1321 ttgaaaaaat aataaatgat acaaattcac aagtatttag tatatttgga gaaaaatgta
     1381 gcaagattac tagaatgtat tacttagata gtaagccata tatatttttt actcattata
     1441 ttccttttga tgaaaattta actgttgaaa gttttaataa tgaaagttct ttatatatgt
     1501 acttatatag gagaaatata aatataacta gatttgaaga tgaatttttt atagaagaat
     1561 cagaactaga ggtatcaaag gctctaaatc tagctaaaga catattatta ggaagaaaaa
     1621 gaaagaccta tgatgagaat
//
LOCUS       DAGSHT010000364         1637 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.370, whole genome shotgun sequence.
ACCESSION   DAGSHT010000364 DAGSHT010000000
VERSION     DAGSHT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.370"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..425
                     /locus_tag="MJB72_003697"
     CDS             <1..425
                     /locus_tag="MJB72_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264735.1"
                     /translation="YNIILHWISEYDYNDIDTLTKINNLEIIFTVIEKVVRGLFVEML
                     CKLQNICKINDSTKLHNKLQYIKDILYNGLKENIFLEKLNYKLLDRRLTKKINLYMKK
                     NAELYESRVETVLLNIREYVDLDEFELFIIDKLIQKIK"
     gene            570..722
                     /locus_tag="MJB72_003698"
     CDS             570..722
                     /locus_tag="MJB72_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264736.1"
                     /translation="MNNLNKNTILGEVIITIAACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            1039..1280
                     /locus_tag="MJB72_003699"
                     /pseudo
     CDS             1039..1280
                     /locus_tag="MJB72_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
BASE COUNT          762 a          149 c          194 g          532 t
ORIGIN      
        1 tatataatat aatattacat tggataagtg aatatgatta taatgatatt gatacattaa
       61 ctaaaattaa taaccttgaa ataatattta ctgttataga aaaggtagta agaggtttgt
      121 ttgttgaaat gctatgcaaa ttacagaata tatgtaagat caatgacagt acaaaattac
      181 ataataaatt acaatatatt aaagatatat tatataatgg acttaaagaa aatatatttt
      241 tagaaaaact aaattataaa ctactagata gaaggttaac gaaaaaaatt aatttatata
      301 tgaaaaaaaa tgctgaatta tatgaaagta gagtggaaac tgtactacta aatattagag
      361 aatatgtaga tttagatgag tttgaattat ttataataga taagctaata caaaaaatta
      421 aataataaat ctaatcgtag gtgttatccc tatgaatcgt atccaccatt gaaatccacg
      481 aatacctata tccaattgga tataggtatt tttatacatt tttaatttaa atattattga
      541 taaatacatt taaaaagtgt taacctaaag tgaataatct aaataaaaat acaatactag
      601 gagaggtcat cattacaata gcagcttgtg atgatgaaaa agaaatacaa atcgtaataa
      661 aaaatcaagt tgataatata ttaaaaaata aaaacaaaga atatcgaatt aaaaaaatat
      721 agctcaaaag aagaaccatt acaaagttaa ttgagaggat acactactct tagatataaa
      781 aatgccagaa ctaaatagta tagatatcgc aagaaacata agaaaagtag ataataatat
      841 ttataacatc tctaataaat tatgtacagg aaagatatga agtaagagct tattgatact
      901 tattaaagcc aataaatttt caagaactag aaaaacacat attaacatgt atcaaaaaaa
      961 ttgaaaaaaa taaaaataaa tctaacacat acaaaatcta atctagcaaa aaaatatata
     1021 gaagttcaaa aaaagaacat gctaatacat acaaaaaata aaacttttga tgctatatgt
     1081 aagagaaaat agaaaaagat ttaaatatag aggaacttgt aagatgtcat aaaagttata
     1141 taattaactt aaactatgta gaaaatataa agtcaaatac tgcaatatta gacagtcaag
     1201 aagaagtacc tattagcaga tatagatata aagatataaa agagaggttt ctaaaattta
     1261 ttggtgatag aatatgctaa accatataaa taaaatatta ttaatattat cattgtttac
     1321 accaataatg atactaaaaa gtatattaac ttacgataaa gatacagata agactaagaa
     1381 aatatctttt tttactgtat tattagctta tcttataact aaaattatta tattatttaa
     1441 cgttgataag acccataatt ataatatgaa gaaaaatcta tatgatataa tctattattt
     1501 gatatttatt acttgggaac ttactataat tatttttata agataggaat tgttaaatat
     1561 atagtttcat ttttcttatt tgacgtatat caccctttaa taaaagatgt tatatctata
     1621 aatttatttt ttgtagt
//
LOCUS       DAGSHT010000365         1626 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.371, whole genome shotgun sequence.
ACCESSION   DAGSHT010000365 DAGSHT010000000
VERSION     DAGSHT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.371"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..729
                     /locus_tag="MJB72_003700"
     CDS             <1..729
                     /locus_tag="MJB72_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY8264737.1"
                     /translation="EKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLIYTL
                     SISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHDIFY
                     DYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPPIAM
                     TILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFGFIG
                     VFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(884..1543)
                     /locus_tag="MJB72_003701"
     CDS             complement(884..1543)
                     /locus_tag="MJB72_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY8264738.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
BASE COUNT          499 a          234 c          192 g          701 t
ORIGIN      
        1 gaaaaaaata tgtcaaacat agattttcta aaaactttaa ttcctcatat acaacatagt
       61 ttagataatt tattaaaaga agcaagtaat tttgttggta aaattcctaa aaacttctta
      121 atctatacac ttagtatatc ttctatgcta tttaatatga caatgggatt tatactatct
      181 atatacatta tatatgacaa agaaaaaatt gcattgggat ttaagagatt tctatattct
      241 tctactgcta gaaacaaggc agataacatt atagaatttt ttaggactac tcatgatatt
      301 ttctatgatt atttacttgg tagaatatta gactctctta ttattggtat aattgcattt
      361 ttaggatttc aatttgttat cagaatagaa aatgctttat ttttagcttc aataatattc
      421 ttaggtaata taattcccta ttttggtcct tttattggtg caatccctcc aatagccatg
      481 actatattat atagtccaca aaaaactata tgggtcattg catttctatt tatattgcaa
      541 caattagatg gaaattttat agaaccaaaa gttatgggta atcaagttgg tataggagct
      601 atatgggtaa tatctgctat tttggtggga caatctctat ttggtttcat aggagttttt
      661 ctttcagtac caatagctgc tgttgtaaaa acttatgttg ataaatatat agataatcgt
      721 ttacaatagc ttttctctac tgtgacttta atttttagtt taaaatttaa ctgttattta
      781 ttaaattatt tctcaaaaaa tatctcttag taaaaaaaaa ttgctaagag atatttttta
      841 taatatataa ttttttcatt aaatttatgc atatatttta tacttattga ttatttgagt
      901 tttgagaatt tccaccacta tctgaaccac cagtagtatc ttgtggagtt ggtgctggtg
      961 ttggattact attatttggc ttttcttgat tttgagaaga actgttatta ttgtcagact
     1021 tattactatc tgatttttta tcattgtttt tagaactaga tttattattt ttactacttc
     1081 cactataaga agatttcctt gcatctgatt caactctata cctatcttct ccagccgtat
     1141 cttttgcctt tttctttctt tgttcctctg cttcgttttg ttctttttct aatttagtta
     1201 actcttcttt ctttttagtt gcctcttctt taataacaga ggaaccattc ttgattttct
     1261 ctataagttt aagttccttg attgcaccct cataattttt taattcttca aactccgtca
     1321 tatttgacat tctcatagct tgcatataca tagctcttgc attctcatta gtactatctt
     1381 cttccagtgc agaggataat gattccatag cttttttata gttatgtttt tctaaatatg
     1441 tttttccttg ctctactaaa tttatttcat cttttttatt aaatgaacat cctacaagaa
     1501 atatagataa tatcgatatt aataaaatta ctcttcctct cattaaatcc ctccttgtca
     1561 tatactttaa attttatcat aattataaag tttttaaaat attttatatt tattacaaga
     1621 aatttg
//
LOCUS       DAGSHT010000366         1622 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.372, whole genome shotgun sequence.
ACCESSION   DAGSHT010000366 DAGSHT010000000
VERSION     DAGSHT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.372"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(47..1339)
                     /gene="eno"
                     /locus_tag="MJB72_003702"
     CDS             complement(47..1339)
                     /gene="eno"
                     /locus_tag="MJB72_003702"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBY8264739.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT          472 a          338 c          174 g          638 t
ORIGIN      
        1 cttatattct ctcttcatta atctttttta gtaaccattt taataactat ttttttaagt
       61 tatagaaaga ttttaatcca caatatctag cttgctctcc aaccatttct tctattctta
      121 ataattggtt gtacttagca actctatcag ttcttgatgg agcaccagtt tttatttgac
      181 cagcatttac tgcaacagct aaatctgcta tagtagaatc ttcagtttct cctgatctgt
      241 gagatataac agcagtatat cctgctctct tagccatttc tatagcatct aaagtttcag
      301 ttattgtacc tatttgatta actttaacta atatagagtt agctactcca ttttcaattc
      361 ctttttctaa tctctctgtg ttagtaacaa ataagtcgtc accaactaat tgtaatttat
      421 tacctaattt ttcagttaat aatttccatc catcccaatc ttcttcgtct aaaccatctt
      481 ctatagttat tattgggaag ttgtttgacc aatcttcata taaagcaacc atttcagcag
      541 cagttaactc ttttccttct cctgctaaaa cgtatttttt agtttctttg ttgtacattt
      601 ctgtagcagc aacgtcaaga cctaacataa catcttcacc tggcttatat ccagcttttg
      661 ttatagcttc aactattaat tctaaagctt ctctgtttga acctaagttt ggagcaaatc
      721 caccttcgtc acctacacca caagctaatc ctttttctcc taaaactttc tttaatgaat
      781 ggaatacttc tgctcccatt cttaaacctt ctttgaaaca acaagctcct actggtaata
      841 tcataaattc ttgaacatca acgttgttat cagcatgctc tccaccgttt aatatgttca
      901 tcattggaac aggtaattgt ttagcattta ctccacctaa gtattggaat aatggtaaac
      961 ctatttcatc agcagctgct ctagctactg ccattgaaac acctaatata gcattcgctc
     1021 ctaatttacc tttgtttgga gttccatcta attctatcat gattgcatct atagctggtt
     1081 ggtcaaatgc atccattcct tctatttcag gtgctattat ttcatttacg ttagcaacag
     1141 ctgtttctac accttttcct aagtatcttc ccttatctcc atctcttaat tcaacagctt
     1201 caaaagctcc tgtagatgct cctgatggaa ctattgctct tcccattgct ccatcttcta
     1261 atactacttc aacttctaca gttggatttc ctcttgagtc taatacttct ctagcatata
     1321 ctaattcaat aactgacatt tgtatatctc ccttcgattt tctattagtt aaattttcat
     1381 ttactgtaaa catccgtcta ctattttaat ctactttcta tattatagta cataaagttg
     1441 cttttgaaaa ttaggtacaa atttatttga tgggtaaatt tgtaaaagtt cctattttta
     1501 ttttattttt tataatattt taatttatat aatataaaaa aacttgtcta aaatccaaaa
     1561 ccaatcttaa atacacaaat attataacta gatttaaaac attaaacata aaatgtacaa
     1621 ct
//
LOCUS       DAGSHT010000367         1574 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.373, whole genome shotgun sequence.
ACCESSION   DAGSHT010000367 DAGSHT010000000
VERSION     DAGSHT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.373"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(377..1084)
                     /locus_tag="MJB72_003703"
     CDS             complement(377..1084)
                     /locus_tag="MJB72_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY8264740.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          509 a          271 c          137 g          657 t
ORIGIN      
        1 taaagtataa taaaatatat aaaaaaatat ataaaatggt actataaaat ttttaatctt
       61 catcaatacc ttttttgctt tcattgttaa aactattttt tctattagtt tatttcttat
      121 aaaacaaaca tttagtatta aaatatatat cacacaatag tatttgcaat ttaactttaa
      181 aaaataaaaa tacccaactg acctaagcaa ctcgattggg tataaataat ctaaaaaatc
      241 atttttgtat aaactcttta tttaacttac atttttattt accttctact ttaattcaac
      301 ttatatttct atgcttctta tactctcatt caacttatat ttctatgctt cttatacttt
      361 tataaattca tcacaattat ttagttctcg ctatcaaaga aattgtatta aaatactttc
      421 cattctctac ttccatcatt tttttgtcat ttatagcata tgaattatca caatttatcc
      481 aatccttcca agcttcatca tgacaattta aagcttcaca tttctctaca atcacagact
      541 ccgattcaga ccaaagttta ttccaccatg taattgaatg gaagttcata ttatcttgcc
      601 aaaatggaat gagttcatct ggtatacaat ccacgaaatc ttcttttaat ccaggcattg
      661 ccatagcaag tattcccccc tcttttacaa gaggtgatat atgattgtct aaaaatcctt
      721 ctttgtttcc aaaatagtga taagaatcga tacttatcac cgcatcaaag aaaccttctg
      781 cataaggtag ttcatgtgcc tctgcctgta ttggaaatat tttatcatcc aatttaaact
      841 ctttaaatct tttatagttt tcagttggtt ctatccataa atctgttgca aatacagtag
      901 catcaaattc ctttgctaaa aatatcgaag atataccttt accacaacct aagtctaaaa
      961 tacgcattcc cttttctagt tttatcttac ttgttaattc ttccaatatt tttatacaat
     1021 taggtcccat catatttttc atgagaaagt ccttatcgta tttattactt tttgtaaata
     1081 tcattctatc tttcctttcc ctatcagtta ttatttatta aattttatac gtttgatttt
     1141 ttatttttta tatgcttctt atattgagca atctcttttt tattcattct ttaacagaca
     1201 attttaatgt gtaattttat ccactctgtg atagtaactt taatgaataa tcttatctac
     1261 tgtgatagtt attttagtga gtaattttat ctactccaca gcaatcgctt taatgagtaa
     1321 tcttatccac tctatgatag ttattttagt gagtaatttt atctactcca cagtaatcgc
     1381 tttaatgagt aatcttatcc actctataat agtcacttta atgagtaatc ataattttta
     1441 tatcatttta gatacttact ctaataagaa gtcattttct aatacttaat aagttttaat
     1501 tacttccact ttaacctata tactaattaa cttatttaat ttagtgcata tttgacactt
     1561 taccttttat tata
//
LOCUS       DAGSHT010000368         1508 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.374, whole genome shotgun sequence.
ACCESSION   DAGSHT010000368 DAGSHT010000000
VERSION     DAGSHT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.374"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(35..997)
                     /locus_tag="MJB72_003704"
     CDS             complement(35..997)
                     /locus_tag="MJB72_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY8264741.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(1167..>1508)
                     /locus_tag="MJB72_003705"
     CDS             complement(1167..>1508)
                     /locus_tag="MJB72_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY8264742.1"
                     /translation="NAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKP
                     NGTFYLFPKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPA
                     FEKLAKLYNLK"
BASE COUNT          596 a          206 c          197 g          509 t
ORIGIN      
        1 tgtcttatgt ataactatga tagattatca attactactc cttatacaag gcttcaacgt
       61 gtttattatg catttgagta attatcgtag gtataataga aattattgaa ccaattacta
      121 tcaataaaat tcctataacc atgtttatag ggataatttc tttaagtatt aataatgcta
      181 gtattggaga tattactggt ttaaagaaga atactaaaga tgtaagacta gtagatgtag
      241 cttccattgc cataaagtaa agcgcataac caacacctgt aacaaatacg aatacataaa
      301 tcataatggg catattgtgt aaggtatatc cactaaaaat agggatattt gcaaatacat
      361 gaagcttatt tttatttaat atatctgcga taaaattaat tttagtgagt aaaaccaata
      421 tcaacatttc caaactacca aatataaagc taaagcaggt tacaactatt ccaccaaatt
      481 ttaaggtttt tcttttacca aatacagtat acattgaaaa aataatagca gaagataatg
      541 ttaagaatat tccactaaga gttagttttg tatgcaatgg atttattata actataattc
      601 caagaacttc taatataagt gaaattacat tgtgcctata tattttttcc ttcaaaatta
      661 agtaagctaa aagcattaca aagactggat tacaactgaa caaaacagca actacagatg
      721 cctttgtgtt tagaacagca agttgataca atgacatact tactactaca cacataaaac
      781 ctaaaaatgc aaagtatttt aagtctgatt ttgtaataga aagactaaga gctcttaatc
      841 tttttacagc aaaaggtagt aagactaaac cgccaacaaa aaatcttgat aaagttattt
      901 gtaagggatt aaagtcatta gtgacagttt ttagactaat ttccattgaa ctaaaaaata
      961 aggtagtcag tattatataa acataacctt ttttcaagtc aaacacctcc ttgtaattta
     1021 tattagtact atagataaga atattcaagc atattggaat aatatggtat gtataaaata
     1081 tagtacataa aattataaac taccttaata tagaatgtaa gtattctata ctaaggtagt
     1141 taattatttg aattactttg atttagttat tttaaattat ataatttggc taatttttca
     1201 aatgctggta aagaactttt aactttatca taagatacac aatatgatac tctaaaatga
     1261 cctggacaac caaaagaaga acctggtact aataataaat taaattgttt tgcatcattg
     1321 cagaattttt tatcatcttc aattggagct tttgggaaca aatagaatgt accattaggt
     1381 ttaacacaag aatatcccat atcgattaaa ctattatata gaaggtcaaa attcttttta
     1441 taaatattta catcaacttc tgcatctaaa cttcttgcaa taaccctttg aaaaagagaa
     1501 ggagcatt
//
LOCUS       DAGSHT010000369         1500 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.375, whole genome shotgun sequence.
ACCESSION   DAGSHT010000369 DAGSHT010000000
VERSION     DAGSHT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.375"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            232..>1500
                     /locus_tag="MJB72_003706"
     CDS             232..>1500
                     /locus_tag="MJB72_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY8264743.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTL"
BASE COUNT          601 a          152 c          197 g          550 t
ORIGIN      
        1 tttacagata gtttactact gaaaataaag aatagaataa atttaaatta agcataataa
       61 aatataaata ataaagttcg cattaacaaa ctatttaaat atcaagaact gttaaaaaac
      121 aacagcaata aacaaataaa atagataaat atatccaatt aaggtataat taagttttat
      181 atgatatagt tatatttgat aatttacaat agtaaaactt ggagggaaaa aatgggtgaa
      241 aagaaaataa ataatatgaa ttattcacat aataattatt ccaaaaagac aggtgaacca
      301 actagaaaaa taaaaaatag ccatatcact cttaaaagta aagaagaaaa aatttgttct
      361 agttgcaata aagaaaataa tactaaggat aattactgta aattttgtgg aaatgaactt
      421 tatgaaatag catcattaag accacttgaa acaaaactag acttgaaatc taaaataaaa
      481 gagctttcat atcatgcaaa taaaagaggt gtatttttaa caacatttac aaccatattt
      541 atattgttta ttattgcatt gatatttaaa gctattataa ctattcaatt taatgatatt
      601 agttatttag taaatccagc acatataatc ctagctctca acttgggtca aattagtgta
      661 tctatgtcta ccatgatggg ttctgggttt ataaatgcca atataggttt gttgatatta
      721 cttataatcc ctattttggt attattaata tcaaacctaa tttttatgag aaaaagatgt
      781 agagattcaa aaactgtatt agcaaattct ctaggagttg gaatttttta tggattagtt
      841 ttagctattt tatctatttt tactaatgta aaaactagtt cacatagtat gttagaatat
      901 ggatatgcat tagaatatag ttatgaattt tttagtgttc ttttaaatgg attcgtatta
      961 gggtttatat gtacatacat aactacttat aaaaaaagtt atgaaaaaga aaatatgtat
     1021 ttatctcttt tttcaaattc tataagaaca ttcatattag gttatgtttt agtattagta
     1081 atactattgg tactaactat atctgatagt agttacttaa atgaactaga tatgagtagt
     1141 tattcaaatg gattaaattt atttactata ctcccacaaa tagctagtta tatgtgggct
     1201 tttgcaaatg gaatttcagt aaccataata aactcaacag tatcaatgtt tactttaagt
     1261 tcttcatctt tatttggaga tactaaatta atgttttatg caatgggagc attgtctatg
     1321 ttaatattat tattaaatgg atacaagcta agatttaagt ataatacaga tagtataaga
     1381 cctataatag tatttagtat atattatgca tttttgatgg gaatattggc tttatttagt
     1441 acatttatac tagatagtaa tattaatttc tttaatacaa ctaattatgg aacaacttta
//
LOCUS       DAGSHT010000370         1472 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.376, whole genome shotgun sequence.
ACCESSION   DAGSHT010000370 DAGSHT010000000
VERSION     DAGSHT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.376"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..714
                     /locus_tag="MJB72_003707"
     CDS             <1..714
                     /locus_tag="MJB72_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY8264744.1"
                     /translation="NISDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEEL
                     DLIDKKIAEFISHKENIIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDED
                     FDFLIQKLQKEYEDRFNILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIV
                     FDEGYYVTLNYTGSNSNNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFV
                     TEIQVPITKYLYFNGLDGK"
     gene            829..1068
                     /locus_tag="MJB72_003708"
     CDS             829..1068
                     /locus_tag="MJB72_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264745.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1239..1415)
                     /locus_tag="MJB72_003709"
     CDS             complement(1239..1415)
                     /locus_tag="MJB72_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264746.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
BASE COUNT          621 a          135 c          231 g          485 t
ORIGIN      
        1 aatatatcag atatatggaa attaaattta ataaaagaat ttagaagctt gaactttcca
       61 atgaagaaaa taaaagaata cctagatgac agaagtatag agtctacgaa aaatatttta
      121 aatgaagaac tcgatttaat tgataaaaaa atagctgaat ttataagcca taaggaaaat
      181 attataaaaa gattgagttc aattgaaagt gtaattcaaa atacaaaaat agatgagata
      241 gaggttgtat atatagaaaa aagaaaagct ttagaattaa atgctgatat aaagagggat
      301 gaagacttcg attttttaat tcaaaaactt cagaaagaat atgaagatag atttaatata
      361 cttggaaaca acaatattgg ttctgccttt tctactgaag caattaataa aggcatattt
      421 aatgagttta aatctgtatt ttgttttctg gaaagtaatg agaaagtata taatatagtt
      481 tttgatgaag gatattatgt aactttaaat tatactggga gtaattctaa taataagatg
      541 tatatggaga aggtgtttaa atttatagaa gaaaataatt ataagataat tgctgaccct
      601 atagaaatct ataagataga tatacatgag actggaattg tagaggagtt tgtaacagaa
      661 attcaagttc ctataactaa atatctatat ttcaatggat tagatggtaa atagaatgaa
      721 aaaatgttga tttggcaccg atttgccact gtgttataaa atcggtagca aattatattt
      781 taaattataa gatagcatga aaaaataatt gtttaggggg ttataaatgt gaaatgttct
      841 aattgtggaa gtgtaaatat tgaaaaggga attttaacaa ctggtggcat atatgctgaa
      901 actattggat taaaatatag aggagatgga aaattagata aagttataaa taaaactttt
      961 ccagtacaat ctactatata ttcagattta tgtttagatt gtggcgaaat agttagaacc
     1021 tatataaagg ataatactga taggaattgg tgtaaagata agaaatagaa taatttttga
     1081 agttgaattt tattaaagat acaagtgaaa aagtataaat gggaagaaat tgttatatgg
     1141 aagaaaactt tgcatatatg gaagattagt tttttaatat aataacattt ttaaatgtag
     1201 ataaaactaa aaagggttct ctagaaaaca atttctattt aattatactt acttaccaat
     1261 accatcactt gatgtaaaca agattctact acattttctc cgtactctag tataaaatca
     1321 tctagctgtg tatctataaa atcacaataa tcatataaac tactaatata agcagttaac
     1381 atgtgtgtac atttatccat ttcatctcta aacatatata ttactatccc cctaaaaaac
     1441 tttcataaaa caaaaagaac aatatctttt tg
//
LOCUS       DAGSHT010000371         1464 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.377, whole genome shotgun sequence.
ACCESSION   DAGSHT010000371 DAGSHT010000000
VERSION     DAGSHT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.377"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(308..526)
                     /locus_tag="MJB72_003710"
     CDS             complement(308..526)
                     /locus_tag="MJB72_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264747.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(527..934)
                     /locus_tag="MJB72_003711"
     CDS             complement(527..934)
                     /locus_tag="MJB72_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264748.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1020..>1464)
                     /locus_tag="MJB72_003712"
     CDS             complement(1020..>1464)
                     /locus_tag="MJB72_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBY8264749.1"
                     /translation="KNNKYLSNDYSFILEDFDIPYKWMYSQMTNRIYEFDDSMIWVWT
                     KRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMDIAVELYEDEQIEKKV
                     SWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFVAK"
BASE COUNT          497 a          251 c          123 g          593 t
ORIGIN      
        1 tttttcatct atgagtcatt tattctctaa tctattagct tggtttttat ttataagtca
       61 tttattctct aatctattag cttacttctt aatctataaa tcattcattt tttaatctat
      121 tagcttggtt tttacctata aatcatttat tctctaatct ataagctttc tttttcatct
      181 ataaatcatt cattctttaa tctatgagct tggtttttca tctataagtc atttattctc
      241 taatctataa gcttggtttt ttacctataa ttaattcatt ctctaatgca taagtaatac
      301 atgaaattta aatattattt tcttcaataa attcaaacaa ctcttctaca gttgtatcaa
      361 ataccttagc aatatccatt gccagtttta aagaaggatt gtagcgacca ttttctaaat
      421 gtacaatagt ttcacgtctt actcctactg actttgcaag ttcaccttgt gtcataaggt
      481 tcttttctct ataggattta atttttgtct ttagtaatac tgacacctaa ttgtatatcc
      541 cctttctgtc aaaatatata aatagaatta acttcaacaa ggattgaata aacaaaatga
      601 ctaataaaga cattcctata tttaaatttg acacatttat gtccaaagtt acaagaaata
      661 tagatactat agctacagaa aaaaacaata cttttattgc aatatcatta actcttgata
      721 gtatacattt tgatgcctca tcaattaaat catcatactt tttattatag acccactgag
      781 ctcccatagc taatacccaa ataattatag gaagatattc aatactactt cctttaaaaa
      841 aatacttatt tactaatgta gctccaatac aataagaaat tcccaatatt tctgaaataa
      901 gtgttgattt aataccagtt tgttgtttag acatataaaa tcccccttaa aaagttatgt
      961 atttataaca cctcttgtta taaatatata acttttttga ataaatttca ataaaaatat
     1021 tattttgcaa caaattcttt tattaatttt acattagata gaggcacttt agatgtcact
     1081 ccttgcaatg tcaaatcttc tatattaact tcatttaatt ttgaacacag ttctaagtca
     1141 aatattcttt cccaacttac cttcttttca atttgttcat cttcataaag ctctacagca
     1201 atatccatta aaggtaaatg ccataaatca aattctgata ataacacatt atcttcactt
     1261 atttctattt caagtaaaac accttttttt cctttttcta aataaccact acatcttaaa
     1321 tcaggtctct tcgtccaaac ccatatcata ctatcatcaa attcatatat tctatttgtc
     1381 atttgactat acatccattt gtatggaata tcaaagtctt ctaatataaa agaataatca
     1441 tttgatagat atttattgtt ttta
//
LOCUS       DAGSHT010000372         1463 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.378, whole genome shotgun sequence.
ACCESSION   DAGSHT010000372 DAGSHT010000000
VERSION     DAGSHT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.378"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            102..863
                     /locus_tag="MJB72_003713"
     CDS             102..863
                     /locus_tag="MJB72_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264750.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            866..>1463
                     /locus_tag="MJB72_003714"
     CDS             866..>1463
                     /locus_tag="MJB72_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBY8264751.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKTEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYD"
BASE COUNT          628 a          125 c          255 g          455 t
ORIGIN      
        1 ataaaattat aacaataagt gtgtgggcag aaaaattctg ctacacactt tatttatatt
       61 cacataaaaa atttatcaaa aaaaagaaaa aggagtaaaa aatggaattt acaagagaac
      121 catttatact agatgacgat ttgtgtctag ctttggtgga caaaagaatt agttatctaa
      181 tggaaaatga attaaaatta agaaatatag agataataaa aacaatagaa tgtaaagaat
      241 tatatgaaag cataaaatat cacccggata tatgtatttg taatttagga aaaggagata
      301 taattgttgc gccgaatgta tataatcaat acaaagagtt attaagtaga tataaattta
      361 atataattaa gggagaagct aatttattaa atagataccc ttatgatata ccttacaata
      421 ttgcgatagt tggtgactat gctatacaca attttaagta tactgacaaa gcaatattag
      481 attatattga aaataataaa cttaaaaaga taaatataga gcagggctat tctaaatgct
      541 caatctgtat agtagataga acgtctataa taacatcaga taaaggtatt tggaaaagta
      601 tgaagaatac agaaatagag tgtttattga tagaaaaagg tcacataaat ttatttgaga
      661 tgaattatgg atttataggt ggatgtacag ggttgatttc taaggataaa ctagcttttt
      721 gtggagatgt taaaaaacat cctgactatg aacgtataaa aagttttgta gaaagtaaaa
      781 acaaggagat agttacactt agctgtgaaa atttactaga tataggttct atagttcctc
      841 tcatgacaag aaaggagcga taactttggt tgagaaggat aatatttctt ccatgaaatt
      901 tttgaggaat aataatgtaa acgatagatt aatatatacg aatgacattg tagaagtagt
      961 gatagattct gcaaagcaag aagtgttaaa taaaaaaata cttaatggta ctaaaactga
     1021 acaaagagct ttaaatattg atgagatatc aacggaaata actagtatga taataagtat
     1081 aatgaaagag aaacttttga aagaatacat tctaaaatgc tatgggcgta catatcctga
     1141 agataaaaat aatatataca tatattcact gaacatattt gctaaaaaag agatttttat
     1201 gagagaaaca gtatttacta gggtgagaaa ttatatagaa gaaaatgatt ttataaatgt
     1261 agaaggtttt attagattta gaatgagaga atttatgaaa tatatttctg ctataggtga
     1321 tattgcagtt gaggagtatt taataaagaa agaccaagat gaatttataa gagttttaaa
     1381 atactttata gatactcaag aagaaaagat tgatttgctt aaagttcata taatggaaga
     1441 taatacattt gttttatacg ata
//
LOCUS       DAGSHT010000373         1463 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.379, whole genome shotgun sequence.
ACCESSION   DAGSHT010000373 DAGSHT010000000
VERSION     DAGSHT010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.379"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..1256
                     /locus_tag="MJB72_003715"
     CDS             <1..1256
                     /locus_tag="MJB72_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBY8264752.1"
                     /translation="GGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIK
                     DIKEVAVVNGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVI
                     GGTNSVSRAVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQL
                     IDSLAVGVLAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSL
                     QEKTVFEAKTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSK
                     TLTVDMPNGEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDID
                     TLMILASANDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAI
                     DSISQKVNKIQDILDKLGFLKKFLS"
BASE COUNT          605 a          131 c          290 g          437 t
ORIGIN      
        1 taggaggtac tgctgtactt aataaagata tagagaacaa gttaaaaagt aatggactta
       61 atgtagaaag aattaatggt aaggatagat atgaaacttc actgattttg gctaataagc
      121 ttaaggatat aaaagatatt aaggaagttg ctgtagtaaa tggtgaaaaa ggattgagtg
      181 atgctgttag cgttggggca ccagcagctc aaaataagat gcctataatt ttgtctagtc
      241 ctaaggacgg agtagaagca tttgataaat ttataaggga tgagaaagtt ataaaagcat
      301 atgtaattgg aggtacaaat tctgtttcaa gagctgtaga aaagagtctt cctaatgcag
      361 aaagagtgag tgggaaagat agaaatgaga ctaatgctaa ggtcatagaa aagttttaca
      421 ctgatacaaa tttaagtaat ttatatgtaa ctaaggatgg tagtaaaaat gagaatcaac
      481 taatagactc gttagctgtt ggagtattgg ctgcaaaaaa tgagtctcct gttgttttgg
      541 ttggtaataa attaaacaca aaacaaaggg acatacttag tactaaaaaa ttaaatacta
      601 taacacaggt aggtggaaat ggaaatgaag aagcttttga tgaaattaaa agtctacaag
      661 aaaaaactgt gtttgaagca aaaactgttg aggaattaac tgatatgata aatatagcaa
      721 gtcctaatga tattataaac tttaaaccaa aagagaatac tgtaaatgaa gcttttagaa
      781 tggtaacaaa taaaccaata actgtgaata taaagggtga ttgttcaaag actcttacag
      841 tagatatgcc taatggtgaa gtgaataact atgctacttt ggtaaatgtt atagttagaa
      901 atattggaga aggtggattt aataaccatg atacaatcac tatattgtca gttcgtgaca
      961 aaaatggtag agttatagaa aatactagaa attcagatat agatacgttg atgatattag
     1021 cttctgctaa tgatacaaaa ttgattaatg atggatatat aggaaaactt atagataatt
     1081 catctaatag tgatataact aataatggta ctatagataa aaaagttaat caggttgaag
     1141 atttagaggc taaagtagat tcaattgaaa aagctataga ttctataagt caaaaagtca
     1201 ataaaataca agatatacta gataagcttg gatttctaaa gaaatttctt agctaagaat
     1261 atatatattt aagtgaagtg tttgttgtaa ttgttctaaa gttatgtagt taaacaggtt
     1321 tatatacaaa tagatttata gataaatatg tagtttagta tatatttgaa taagctttgg
     1381 ggaggtaaca tggggattgg aattgaatgt aacaaaaaaa taaataaaag tattgacaaa
     1441 actagaataa agaaaaataa tct
//
LOCUS       DAGSHT010000374         1452 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.380, whole genome shotgun sequence.
ACCESSION   DAGSHT010000374 DAGSHT010000000
VERSION     DAGSHT010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.380"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..212)
                     /locus_tag="MJB72_003716"
     CDS             complement(<1..212)
                     /locus_tag="MJB72_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY8264753.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNIN"
     gene            complement(309..>1452)
                     /locus_tag="MJB72_003717"
     CDS             complement(309..>1452)
                     /locus_tag="MJB72_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBY8264754.1"
                     /translation="PAPVIGGLLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIG
                     LEISINLIKKGGSVLFRYWILCGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGG
                     HGYSAAFGLTIENLGIEGAVGVGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNID
                     LSIPRYNRNLKRFGSKFFRNKNRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRC
                     FYITFNILLPSVVTCMFSAVIFRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNI
                     DLFELSTLLPPILLIVTFQVIFIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPN
                     ALANMSSLTQKYGDSPKAFLVVPLVSGFLLDAISIPCILFFINILT"
BASE COUNT          553 a          232 c          167 g          500 t
ORIGIN      
        1 aaatttatat tttttgcatc agattttcta atagctaagt caaaccctct aaatctaatc
       61 actatagggt cattaagagg agctatcttt ttcacttcaa cttcagtgcc atctattagt
      121 cctaaggcta gaagtctctt cataagtttt tcatttccag ctatattgtc tataattccc
      181 ttttgtccaa gttttaagtt gtaaacagtc attattaaca cctcctaatg aaaatcatta
      241 tcaaacttta tatttataat atataacaaa aagagcaaat tgtcaatata taacaaaaaa
      301 taaacatact aagttagtat gtttataaaa aatagtatac atgggatact aatagcatct
      361 agcaaaaaac cacttactaa tggtacaact aaaaaggctt ttggagaatc accatatttt
      421 tgtgtcaaag aactcatatt tgccagagcg ttcggagtag ctcctattcc atgtccaatc
      481 aaaccactta ttatgatggc agaatcaaaa tcttttccaa gtactctaaa gcaaataaat
      541 attgaaaata ggattataaa gattacttga aaagttacta ttaagagaat aggaggtaat
      601 aatgtagata attcaaataa atctatattc ataagtgata aagttaaaaa aattcccaat
      661 gaaagttctt ttaaaaaatc tataagttta aaatttaatt ctagtatatt tatcttatca
      721 tttaaatttc taaaaataac agccgaaaac atacaagtta caacagaggg taatagtata
      781 ttaaacgtta tatagaagca tctacttata atttctcctg tatttataca aataaatatg
      841 agaagaacct gttccaaaaa tacagatggt gttattgttt cggagaagtt attatttttt
      901 atcctattct tatttctaaa aaatttactt ccaaatctct ttagatttct attatatcta
      961 ggaatcgaca agtctatatt tgatgatggt tttaatttat atttatttat tagaaattta
     1021 gctacaggac atcccaatat accacccata ataagtccta ttgttgcagc agataatcca
     1081 accccaactg ctccttctat accaagattt tctattgtaa gtccaaatgc agcagaataa
     1141 ccatgaccac cttccataga tacatttcca cacataagcc ctaaaagagg ttcaagtttt
     1201 ataatctttg atattgtaat agctaatata ttttgacaaa atgccaatac tccacacaaa
     1261 atccaatatc taaataatac actaccacct ttctttataa gatttatgct tatttcaagt
     1321 cctatagttg taaaaaagaa ggttatgaaa tttggcatta aatttccact tatagatata
     1381 tctgctatat taaaatattt aaaaaataaa tttagaaaac aaaataatag acctcctatt
     1441 actggtgcag gt
//
LOCUS       DAGSHT010000375         1451 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.381, whole genome shotgun sequence.
ACCESSION   DAGSHT010000375 DAGSHT010000000
VERSION     DAGSHT010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.381"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            288..626
                     /locus_tag="MJB72_003718"
     CDS             288..626
                     /locus_tag="MJB72_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264755.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            648..965
                     /locus_tag="MJB72_003719"
     CDS             648..965
                     /locus_tag="MJB72_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264756.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1115..>1451
                     /locus_tag="MJB72_003720"
     CDS             1115..>1451
                     /locus_tag="MJB72_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264757.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEK"
BASE COUNT          631 a          141 c          209 g          470 t
ORIGIN      
        1 aatatattct acatatctaa tatgtaatta caataaaaaa tgctataatt aacatattat
       61 taattcgttt tattattgta gtatggctat gaaattatga aaatatttct ctaattaaaa
      121 atatgattta tccatatttg tttattgtag atacagacta tacaaaaata aatagcactt
      181 atttcagtat aaaaacatat atagtctata tttagactaa aaaagtataa atagtctata
      241 taaaacaact taaactatga atataatcta aagtggaggg ataataattg gcaaatatag
      301 gaaaaataat aggagataga ataaaagtct atagaacaaa attgggatat agtcaagagt
      361 ttctagcaga aaaggcaggt ttacatccaa catatattgg tcagattgaa agagctgaga
      421 caaatacatc tatcaatatt ataatgaaaa ttgctactgc tttagacata ccacttgaat
      481 tgttatttgc aaatattatt acaacagaag gcgttgataa ctctgtacct ttagaatgtt
      541 atgaaatgat taataaatta actgaaaaag aacaaattgc aatgctagag ttaataaagt
      601 gcatcattaa atacaaagaa atataagtga gggaaatagt aacaataatg aattatatag
      661 gtaaaagact taaagaagaa cggaaaaaag caaatttaac ttcaaaagaa tttgcaaata
      721 tggttggagt atcgccttgg tatataactc aaatcgaaag tggtaaaaag aatccaagtc
      781 ttaaaacatt cataaaattt gtaaatatat taaatatttc tgcagatgtt cttataaaag
      841 atattacttc cactggaaaa acatatttag aaaatgatat aaatgaagag ttaaaagact
      901 taaattcaag agaactaaat ctgatatcac agattataga catcataaaa aagaataaaa
      961 tataattctt tatattttat ataaaaaaaa aggttatttg aaataaataa cctttttttg
     1021 tctttttgtg ttaacattgt cttatagtgc taatgagtac tattagacca tgttctatgg
     1081 ccttatttta atataaaacg attggagata ggctatgatt gtgattgaag gtagcgataa
     1141 atttaagatt gcaaaagaat atattgatgt agaatatact ctttttagca aagtaacttt
     1201 taggtatgaa aagttgaaat ttaaagataa tgctgaattg gaaaaaatta agatgtttaa
     1261 atataaaaat ggctacatcc ctaataaatt aaacctttct tttggatatg gattctcttc
     1321 ttataaaaag caaataatta gagaaactgt agatacttta agattgacag aaattttttc
     1381 aagcgagaac atagaagata ttaaatttat aaaagatggt acaaaaaaat tagaaattag
     1441 catagagaaa g
//
LOCUS       DAGSHT010000376         1448 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.382, whole genome shotgun sequence.
ACCESSION   DAGSHT010000376 DAGSHT010000000
VERSION     DAGSHT010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.382"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(192..578)
                     /locus_tag="MJB72_003721"
     CDS             complement(192..578)
                     /locus_tag="MJB72_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264758.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENI
                     CDFVTTPIKKVVQLLSFIQNKKLNYY"
     gene            611..757
                     /locus_tag="MJB72_003722"
     CDS             611..757
                     /locus_tag="MJB72_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264759.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
BASE COUNT          566 a          125 c          203 g          554 t
ORIGIN      
        1 tttttgatat aggggtttta gggtctttga agattatttt attgtataca taaactaatg
       61 tttgatttac atgtgagatt ttttatcaag gtctacatct gaccatataa gtgatacagc
      121 ttcaccaaaa cgtaatccaa gagttagcat caaaaaaatt ggtataccta taggaatatt
      181 ttttgatttt tctaataata attgagcttt ttgttttgta taaagcttag aagttgaact
      241 acttttttga ttggggttgt aacaaaatca caaatatttt ctgatatttc ttttaatcta
      301 taagattctt tcaatactga ttttataaaa tcgacttggt tttttattga atttaacatg
      361 tgctcattgt atatgttatt tatataagac tgtaaaatag aaggtgttat atctattaat
      421 ttcatatctt taaagaaaac ttcaatttga tttttaatca tattttttct atttctaata
      481 gtattaggag aaaaactaca ttctctatct tttatatatc tgtaacatct ttctacaagt
      541 gttacattat ttggagcaac aaacttatta ttgtttatag tggactattg gaaaatcgaa
      601 taataataac ttggtattcg aaacttttga taaaacaata gaactatatc caaatgcaaa
      661 acctattttt catagtgata taggatttag attagatgat gaaagttttg aagagtattt
      721 aggaagagta tttagaaaga gatgcaaagg gatataagat tgtaaataaa aatggatttt
      781 ttaatgaatt tgatttttat gaagcagtaa atatatgttt aaatatctca atagaagatg
      841 cgataaaatc atataatata ttagttaaat cattagtaat gttagataaa agattaggta
      901 aaaggcgatt aaaaaatata tatgaagata tagaaaaaga gaataattta gtaaagaaat
      961 tttataatat aagagttgaa tcagaaaata tgattttaaa gaaaaatgca taaaaaatac
     1021 aactaataca gaaataaata aagtaaagtt ccatggaaaa ctgagaaatt taaaaagagt
     1081 atatatattg aaatgtagta atttcaatat atacactctt ttttattttg aactaattcc
     1141 taaacgtcaa tttagagagt aattgaattt taagtttagt taaaatgaaa gttattttta
     1201 ctaaatatta tctttgttgt atagttttat ttataatata aaaaaagtaa ctctatgcta
     1261 ggatttatga ctagaattag aataaaagtt ctagcttata atatttcatt ttttataagt
     1321 aaagttatga gcaaagatga atctatagca aaaattaaag agttaatatt tggatgactt
     1381 atccataaac taacccttag gggtgatttg ttttactaaa atcaatttac tttatatggg
     1441 aattaaac
//
LOCUS       DAGSHT010000377         1427 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.383, whole genome shotgun sequence.
ACCESSION   DAGSHT010000377 DAGSHT010000000
VERSION     DAGSHT010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.383"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            91..372
                     /locus_tag="MJB72_003723"
     CDS             91..372
                     /locus_tag="MJB72_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264760.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            636..890
                     /locus_tag="MJB72_003724"
     CDS             636..890
                     /locus_tag="MJB72_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264761.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            935..1258
                     /locus_tag="MJB72_003725"
     CDS             935..1258
                     /locus_tag="MJB72_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264762.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          601 a          106 c          229 g          491 t
ORIGIN      
        1 agtgttactt tcataataaa tacatataaa gatattttta agaataaaat ttattattaa
       61 ataaaaaata aaggagagtg atttaatagc atggcatcaa aacaaatacc aagaaacaat
      121 ttagaaaagt ttttaagaat aagagtctta tcacatacag gcgataaaat acatataaag
      181 ttacctgtaa attttgcaaa gagaatgatt gagaataatg ctttggattt atttaatgga
      241 aaagatgatg tagtagatgg taaaaaagtt actgaaatat tagttaaggc atttgactat
      301 gatttaactg gagaagtagt tcacttggag agaaaaaatg gagacattat taaagtttca
      361 attgtacaat agaattattt tatatgaatt ttatttttga atagttataa ataattagaa
      421 atttgtaaaa aagtataata tattattagt aatggtacag aatacacatt tataatttat
      481 agatgtgtat tttttgttta aaaaatttaa atatataaaa ctataaaggt tacaattttg
      541 gaaatagatg taaatatatg ttgtattgta taataagagt aatataaaat ctagtaaaaa
      601 taatcaaata aatttagctt atgagaggga taactatgga aaataaggat tttaaattaa
      661 caatatgtat atttgctttt gtaattggag tagtagtatt attctgttgt ataggtttag
      721 gtgaatttag tgcagagcat attattcaac aaaatggtgg aatcatggat actgaccaat
      781 ataatattta tctagaccaa tctatttctc aatatagaaa ttttggttct atattagctc
      841 tgttaggagg aatagggata ttgtttgata aatatatcag aaaatactag agattatgtt
      901 ttgtctaaaa taaattctta tgaggagata aaaaatggca acagaaatat ttactgtaaa
      961 aattacaccc ttagaggcac aaaaaatgat aaaagaaaat cttgatgcag atttagtttt
     1021 tagtgattct tataatttag gagatgataa atttatacta attacaactt ttgagaagta
     1081 ttatgcaaga aataatagtg atgcaggatt aatagtagtt tatgaaaata ccacaggaaa
     1141 aacagttatt aaagctactt caacaggcag tgcaattgga attttaaaaa ttgattgggg
     1201 tgcaggtgga aatttactta aaagagtaaa aaacatactt gctgattata ttatttaatt
     1261 attttatcta actgataata tgtcaagaaa atctcgtaaa agaggttaag aacattttta
     1321 taatatatag ataaataaga atttgaaggt aaagttttta aggtataaag aaaaataata
     1381 taataaaacg aattggcttt ataattcttt ataaggcaaa gagcaca
//
LOCUS       DAGSHT010000378         1398 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.384, whole genome shotgun sequence.
ACCESSION   DAGSHT010000378 DAGSHT010000000
VERSION     DAGSHT010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.384"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            214..759
                     /locus_tag="MJB72_003726"
     CDS             214..759
                     /locus_tag="MJB72_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264763.1"
                     /translation="MKDELQIGKNNIAGTLGSPFVGTDKLSVTKTKIQTLKNTLAISL
                     SMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGEKTFQLYQVDNNTPKTCSANA
                     ISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITAVPQPSNFGEMYMPNTFDCVV
                     GHTCILPVKDYKIPHFFYAIA"
     gene            783..1088
                     /locus_tag="MJB72_003727"
     CDS             783..1088
                     /locus_tag="MJB72_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264764.1"
                     /translation="MTEYIEVGRRIFFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDL
                     EYDYIDYDKYKIKGINIETKQPILEEIPIYMSEEEKRIQELENQILIAENEKVGGIL"
     gene            1088..1252
                     /locus_tag="MJB72_003728"
     CDS             1088..1252
                     /locus_tag="MJB72_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264765.1"
                     /translation="MNINNIVVRILAERILNKGLNPLKNRPFELDDVTNIEYRKAVDD
                     YINTIEQKIK"
BASE COUNT          598 a          160 c          207 g          433 t
ORIGIN      
        1 aaaagtgatt gaatttaaac aatttttaca agatatatat agtatagata aatttatttt
       61 aaatagaaaa agaagcaaaa taaacaagta taagactttt tagtaaccat ttctcaacaa
      121 atagttacta aaaatagaaa ggtgatattt tttatgatag aaaaattaaa cgaaaatgca
      181 agtttaagtg acttaataac cacttttgag aacgtgaaag atgaattgca aattggtaaa
      241 aataatatag ctggtacatt aggaagtcct tttgttggaa ctgataagct aagtgtaact
      301 aaaacaaaaa tacagacctt gaaaaataca ctagcaatta gtttgtctat gaaaaatata
      361 tctgctcaat caactgaaac acttcattca ctaattggta aagtagctgg aatcgcaaat
      421 atttttattg cacaaggaga atgtactcaa gttggagaaa aaacttttca actctatcaa
      481 gtagataata atacgccaaa aacttgttca gcaaacgcaa taagttttaa cttagctttt
      541 actccaaaat ttattattgt ctatagaaat gcttacaata taggtttgtg tagttttgtt
      601 ttttactcat cttttcgtaa tattataaca gcagtgcctc aacctagcaa ttttggtgaa
      661 atgtacatgc ctaacacatt cgattgtgta gtaggtcaca cttgtatttt accagtgaaa
      721 gattacaaaa taccacattt tttctatgca attgcttaaa aaataagtta aaggaggaaa
      781 ctatgactga atatatagaa gtaggaagaa gaatattttt tgatgaagaa ggcgaaataa
      841 tattctatga aggacaatcg aaaggaaatg ttccagagcg aaaaaatatt aaaaaaatag
      901 aatatataga tttggaatac gattatattg attatgataa gtacaaaata aaaggtataa
      961 atatagagac taaacagcca attttagaag aaataccaat ctatatgagt gaagaagaaa
     1021 agagaataca agaattagaa aatcaaatat tgattgcaga aaatgaaaaa gtaggaggaa
     1081 ttttataatg aatataaata atattgtagt aagaatatta gcagaaagaa ttttaaacaa
     1141 aggactgaac cctttgaaaa acagaccttt tgagttagat gacgtgacta acatagagta
     1201 cagaaaagca gtagatgatt atataaatac aatagaacag aaaataaaat agtaactttt
     1261 agtaactctt cttaaagaat aggtcactaa aaaattttaa ttttgtttat tttacttact
     1321 ttcacatact tataaagaaa tcattctaaa ttctacatta aaagaaacgt tcaaatttaa
     1381 tcatctttct actttaaa
//
LOCUS       DAGSHT010000379         1394 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.385, whole genome shotgun sequence.
ACCESSION   DAGSHT010000379 DAGSHT010000000
VERSION     DAGSHT010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.385"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(395..1234)
                     /locus_tag="MJB72_003729"
     CDS             complement(395..1234)
                     /locus_tag="MJB72_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBY8264766.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT          561 a          201 c          124 g          508 t
ORIGIN      
        1 atttattatt tgtttataat tatttatgtc gcttttttct aaatctaatt acagtagctt
       61 attctaatat attggtaatt agaaatacag gcgcatataa aaacacatga ctaaatactg
      121 cattatttga aaatacagaa attaactcat taggactata atttttattt agtatatttt
      181 ttgatactta ccaattttat ctcttactta tcaatagtca tctttttaaa gatgattata
      241 aattaaaact atcccattaa ataaaatttg tcaagttata ctaaaaataa aaaaagccca
      301 gaaaaattct agacttttta acttaaatgt caatatttct ttattaattc aattacattt
      361 ataatactaa atactctctt ttattttaaa tactttactt taacatttca tgtcttatct
      421 ttctaaaata caatcttgaa tttacctcat agccaataac gatagaccaa ctactaaaat
      481 acatccaggt cataagtaca attatttcag ctatacttcc atagattact tcatagttag
      541 catagtaatt tgtataataa gaatataaaa atgatactaa aaaccaagca aaagtagcaa
      601 aaatagcacc tggcgcagat tctttaatag ttaacttttt atttggagtg tatttatata
      661 aaagtgtaaa tatcactatt attgttatta tacctacagt gtatcttaga atatcccata
      721 tttttataaa tatctcatcc aatcctacaa gattaaaaat aaaataacct attttttccc
      781 cataaactaa aaatatcata gataaaaaaa taaggaccaa aagcattatt gtaaataaaa
      841 atgatataga taaaatttta aaaaaagacc tagtttcttt aactttatag gctctattca
      901 ttcctttaat aagagctttt actgctcttg aaaatgtcca catagtaagt acaaaactga
      961 ctgctaaaaa tttcaggttt ctattgtcta ttgcagaaac tatgattgaa gataacacag
     1021 caaaagcacc ttctggcatc atatttctaa acaaagctat atacttattt aaatgaagaa
     1081 tcggtatata agctatagaa cttattgtaa atattaaaaa aggaaatatg gacaacatta
     1141 agaagaaaga tacttctgct gactttgaac ttaattcact gtaattggat tttgcaatta
     1201 tatattcaat caatttctta tctataccaa acataaatat ccccttttct atcaaattaa
     1261 tttcattata caatattaat tatactcaga ttcattctat aatataatta tttaagtaaa
     1321 aataacacaa ataaaagctg aaaataacaa ttctactaac atttccatat tctactcaat
     1381 gaaagtaaat tata
//
LOCUS       DAGSHT010000380         1361 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.386, whole genome shotgun sequence.
ACCESSION   DAGSHT010000380 DAGSHT010000000
VERSION     DAGSHT010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.386"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            39..332
                     /locus_tag="MJB72_003730"
     CDS             39..332
                     /locus_tag="MJB72_003730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264767.1"
                     /translation="MIDLLIVERKTEDIKFKNPFVYMITFVSCFIFLFTVYNTTNHII
                     INLIDDKQLNSKSKGVEINEFTYQDWQQFCDIKYYGKKGLISMTLKEYIEKYK"
     gene            587..1354
                     /locus_tag="MJB72_003731"
     CDS             587..1354
                     /locus_tag="MJB72_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264768.1"
                     /translation="MKNILSVEKIEKYYGNKDNITKAIDNISFKVDEGEFVGIMGPSG
                     SGKTTLLNCISTIDNVTIGKIIINNNDITRLKSKNLDKFRQNELGFIFQDFNLLDTLT
                     AYENIALALTIKGEKPSKIDSKIRDVATYLEIEKVIDKYPYQMSGGQKQRVASARAIV
                     TNPSLILADEPTGALDSKSARLLLERFESLNQDLKATILMVTHDAFTASYAHRILFIK
                     DGKIFTELVRGADSRKEFFNRIIEVTTLIGGDDNDVF"
BASE COUNT          605 a          132 c          205 g          419 t
ORIGIN      
        1 taatgatatt taatatctat ttaatttcta aatacaaaat gatagattta ttaattgtgg
       61 aaagaaaaac agaggatata aaatttaaaa atccatttgt atatatgatt acatttgtat
      121 catgctttat tttcttattt actgtatata acactacaaa tcatatcata attaatctaa
      181 tagatgataa acaattaaac tccaagtcta aaggtgtaga aataaatgaa tttacatatc
      241 aagattggca acaattttgt gatataaagt attatggaaa gaaaggttta atatcaatga
      301 ctttaaaaga atatatagaa aaatacaagt agaataaaat aaattttaga tggaaggcat
      361 tgccctctat atttttgtgt tttctagaat tgagtaaatt ttgttactct agcaaagtat
      421 aaaagaaaga atgctggtct tgagtatttt atattagtaa ttattaggga ataatatata
      481 attactacaa aaatgaaaca ttaatgaaat gtaaatcaat ttaaaaaaat taaataaaaa
      541 attacaatag agatataaga aaattaattt aaaggagaaa tagaacatga agaatatatt
      601 aagtgttgaa aaaatagaaa aatattatgg aaacaaagat aatattacaa aagcaataga
      661 taatataagc tttaaagttg atgaaggaga atttgttggg ataatgggtc caagtggtag
      721 tgggaagaca acacttctta actgtatatc aacaatagac aatgtaacaa taggtaaaat
      781 aataataaat aataatgata ttacaagatt aaaatctaaa aacttagata agttcagaca
      841 aaatgaatta ggatttatat tccaagattt taatctttta gatactttaa cagcttatga
      901 aaatatagca ttggcattaa caattaaagg agaaaaacca tctaaaattg attcaaaaat
      961 aagagatgta gctacatact tagaaataga aaaagtgata gataaatatc cttatcaaat
     1021 gtcaggagga caaaagcaaa gagtagcatc agctagagca atagttacta atccatcatt
     1081 gattttggca gatgaaccta caggagcact tgactcaaaa tcagcaagat tattacttga
     1141 gaggtttgaa agtctaaatc aagacttaaa agcaacaatt ttaatggtaa ctcatgatgc
     1201 ttttacagct agttatgccc atagaatcct atttataaaa gacggaaaaa tatttacaga
     1261 attagtaaga ggagctgatt caagaaaaga gttttttaat agaataattg aagtaacaac
     1321 tctaatagga ggagatgata acgatgtatt ctaaaatagc a
//
LOCUS       DAGSHT010000381         1303 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.387, whole genome shotgun sequence.
ACCESSION   DAGSHT010000381 DAGSHT010000000
VERSION     DAGSHT010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.387"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(212..928)
                     /locus_tag="MJB72_003732"
     CDS             complement(212..928)
                     /locus_tag="MJB72_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264769.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT          477 a          188 c          144 g          494 t
ORIGIN      
        1 tgataatata atacctatgc agacaaactc taattctact tttgacaaat gtacaggagt
       61 accgaaaaaa taaaaataag ggagagcaaa ctctttataa taacaattaa tattataaga
      121 aaagttatta ttcaaatcat aaactgcttc aaataatata gaaactaata gaaacagtaa
      181 taataataaa ataaattttg ctttagaatc attaataatt aagtttttca attttgtaac
      241 caatgcccca caccaccttt aaatattttg gttcttttgg attaatttca atcttttctc
      301 taattcttct aatatgaaca gcaatagttc tctcacaact ataagcatcc tcattccaga
      361 cattttcata aatctcatga gaagagaaaa ttttaccttt tcgtgacata agaagtgtaa
      421 gtattttata ttcaagtggt gtcattttaa tttcctcacc atctaaagaa actatttttg
      481 tatctgtatt tagctctagg ccaccacttt ttagaatata attagtatca tttgagttat
      541 ttgtattact aaaatcacct aaatttatat accttctaag ttgagatttt actcgagcag
      601 taagttctag tggattaaat ggtttagtta tataatcatc agcacccata ttaagaccta
      661 taattttgtc catatcttct gattttgcag ataagagaat tattggtata ttcctttttt
      721 ctcttatttt aagtgtagtt ttaagcccat ctaaattggg catcattaca tccattatta
      781 ttaacttaat atcattttcg attagtatgt ccaatgcttc aagtccatca tatgccttta
      841 aaacattaaa gttatcaggt ctcaaataga actctatagc atcaactatc tctctatcat
      901 catctacaac caatatattt tgattcaact tattaacccc cttgaaattt tatttttaac
      961 caatttttat tttataccaa aaagaggtat tagaaaacag taagcccgtt cattatgaaa
     1021 taaaaactag tacttttaat atattgtcaa tgcatatagt aaatttaaaa gtatagacgc
     1081 ccatattaat ctgaaggtaa aagtttatga aaatatctag ggtaatctat cacacttctt
     1141 atgttatttt ggtttattaa ttttttaggt ttaaacatat attaacaaat ataaataaca
     1201 aatgagtttc ataattatta agaatttata acaaatattt attttaatgt agtttaatat
     1261 aaacaggtag tttaatgtag gtatgtataa agcatttaat aag
//
LOCUS       DAGSHT010000382         1301 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.388, whole genome shotgun sequence.
ACCESSION   DAGSHT010000382 DAGSHT010000000
VERSION     DAGSHT010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.388"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            15..161
                     /locus_tag="MJB72_003733"
     CDS             15..161
                     /locus_tag="MJB72_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264770.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(266..790)
                     /locus_tag="MJB72_003734"
     CDS             complement(266..790)
                     /locus_tag="MJB72_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY8264771.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(843..1067)
                     /locus_tag="MJB72_003735"
     CDS             complement(843..1067)
                     /locus_tag="MJB72_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264772.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
BASE COUNT          413 a          209 c          114 g          565 t
ORIGIN      
        1 taggaggctt aaatatggat atggatttac tctttaaaat agtatttgct atagttgcta
       61 taggtgtttc tgtaaaaatc attaaaactg ttgctagttt agcttttaaa atagcgttga
      121 tattgctaat aattttattt gtatatagaa tgtttttgta aacatttaaa aataagtttt
      181 ttaacacaat agagaagtaa aatagattat ctttaatgta aattttttat taaagacact
      241 ctattttact tctctattag cttctttaaa ttaaattttt attatatctt cccataatat
      301 aaacatcttc aaattttcca tctctgatta cagcatattt tttagttccc tctatttcaa
      361 aacctagctt tttatataaa tttaatccct tttcattatc cacaattact tctaatccta
      421 ctctagttaa cataatccaa ttatctgcta tatctaaaag ttgttccata agcttcttac
      481 ctactcctct tccctgataa ttctctgcaa cacttattcc tatttctgcg ctatgtctca
      541 atcgtggaga agaatttaca tgtaacccag caaaacctat tacatctttt tctccattat
      601 tattagtttc agctacaaac atataatcat tttctcccaa agaagctaac atagcttcag
      661 ttttatcgac tctgtcactc attaatgcca aagtatttct cataacactg tatgttcttc
      721 taatttcatt aaaagcttgt gcatcttctc ttcttatagg tcttattgtt atatctatag
      781 gcttactcat attggattcc ccctcaaaca taatctttat ttttaattaa aattttacat
      841 ctttatttta tactgttcaa atctatgtat gcttctacct gaggtacata tatcatatta
      901 tcttttatat gtgtacttcc aaattctttt aattctgtac cttctttatc cttagttatt
      961 ttatatctaa tggattccat accttcaact acttcagcat gaaaaaacat accttcatat
     1021 tgtttaaatc taaaattaaa attgtcttta gtaagtttta ttatcatatt tttatctact
     1081 ccttatataa ttgatatttt tcttattatt agttgtatat atactgctta ttcatattaa
     1141 tatattataa cttatcattt atttagtata aagttataaa tagatatatt tatacttatt
     1201 ttacaatctt tcttcaattg atatataata tgtttatata aactaatgta tgtgaggtga
     1261 aatatgagca aaaaaacaaa atatttttct ttaattctct t
//
LOCUS       DAGSHT010000383         1283 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.389, whole genome shotgun sequence.
ACCESSION   DAGSHT010000383 DAGSHT010000000
VERSION     DAGSHT010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.389"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(48..332)
                     /locus_tag="MJB72_003736"
     CDS             complement(48..332)
                     /locus_tag="MJB72_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264773.1"
                     /translation="MFVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            539..>1283
                     /locus_tag="MJB72_003737"
     CDS             539..>1283
                     /locus_tag="MJB72_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY8264774.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSELSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEE"
BASE COUNT          547 a          116 c          173 g          447 t
ORIGIN      
        1 ttttattatt aattttatca gtgttttatt ataaatagct tacataatta cacatttttt
       61 ctgattaaat aatatgatgc tattgttata atagatacta atgctaatga tgttattgca
      121 gtatctaatc ttcttattaa taaactcatt tctatatatt caagttctat taaatattga
      181 taaaatattt ctagtttaac agctttttct ctatctatat ttccatactt taactctaat
      241 ttatctaaag attttttatt acaataatta actttatgtt taattaaata tcttattgta
      301 gcacctatta caattttcac tctaacaaat ataaatataa tattccatcc aaaagttaag
      361 aggggatatc ttttttatga tgggaatggt taaaatattt ataactgaca tctaactgat
      421 atttaagctt taaaatcaaa accatattga ttttctaaaa taataactac ttgatttagt
      481 aaataataaa gaatatagta actatataga caacaaaaag aaaaggagtg tataaccctt
      541 ggaaaaacag tattttacaa caggtgaatt tgctaaattg tgtggtatat ctaagcagac
      601 attaattttc tatgacaaaa taggtatttt ttctcctgaa tataaagata aaaataatta
      661 tagatattac tcagtttatc aatatgatac acttgatata ttacaatcat taagagaaat
      721 aggaatgagt ttagaggaaa taaaagagta tatacaaaat agaactcccc agctttgtgt
      781 gaaaatgctt aaagaagaag aaaaaaagat aagagaaaaa ataaaaaaac taagaaagat
      841 aagttctaag atgcaaaata gaataaatat aacagttgaa ggaatttcta aaatgaatag
      901 tgaagaaatt tatatatcca aaaagtttga agaatattta gttctatcaa gcgagttgag
      961 taatatgagt aatagtgatt ttatgatgga attaatagat tttaccaatt attgtaaatc
     1021 gaaaaatttg tatcaaggat atgagttggg agtaatagta agtaatgaaa atataaaaaa
     1081 tggggactat ttaagtatat caagttttta tttgaagata gataaaaaaa taaaggataa
     1141 aaagctatat gtaaagccag aaggaatgta tgcttgcata aaacatattg gaaaatatga
     1201 ggattcatat aaatcttacg aaaaattgaa aaaatatata tatgaaaatg aatataaaat
     1261 tattggaaat tcgtatgagg aga
//
LOCUS       DAGSHT010000384         1281 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.390, whole genome shotgun sequence.
ACCESSION   DAGSHT010000384 DAGSHT010000000
VERSION     DAGSHT010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.390"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1130)
                     /locus_tag="MJB72_003738"
     CDS             complement(<1..1130)
                     /locus_tag="MJB72_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY8264775.1"
                     /translation="MIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYT"
BASE COUNT          469 a          168 c          172 g          472 t
ORIGIN      
        1 gtataagaaa tatccaattt tccattgtac ttattaacaa tttctctaat attctcaata
       61 ccatatccgt gagaattact atctttttta tctgaaataa atagttcttg ttttctattg
      121 ataggattta ctttgttgtt tgtcaataaa tagctaaaat actccttctc acaacgtgcc
      181 tttaatacca cttttctatt tgacaaatca ggaattttca aagatgcttc tatagcgtta
      241 tctaaagaat ttgaaaaaat agtacataaa tctataggtt ctatacccaa gtgattgtct
      301 atatctatat taaagtgcac atcaacacca ttttcttcca aacttgtatg acgaagattt
      361 aaaattgagt taagagcttg gtttttgcaa aattgtttgc cactaagagt acctatacct
      421 atttttaact gattaaaata ctttttagct ccagcaatat cttcaatttc taaatacgat
      481 tcaatcattt gaaagtgatt attcatatca tgtcgcattt tgcgtatttg aatttgttga
      541 ttttctagta agttatagta tttttctttt aggtgatttg cttgtttttc tatcttcatc
      601 tttacatttt caatcaatgg ctgtaaaaga agtatcacac cgatatttgc cagaattgct
      661 gacaagatga ttagataagt gccataaggt aagttgccta agataatatt cttattacta
      721 cccataggga ttaaattagt aggtggcaga ataatagcaa ttaatattga taaaaatgaa
      781 gatatacata ccatatcaat aaaaatccat attttatttg ataagtattg ttttatattg
      841 accaatctat tctttaggaa aatatgtatt aaaaaccaaa ataataattt aattagagca
      901 gaaattatct ggactattga aagtattaaa atatactctt ttgagtgtat aagttcctca
      961 tatatagggt tgtttataaa aagaaaatta agcccaatta taataggata aagtatcatg
     1021 acaactgata ttataggaat taatttgctt ttgctacata ttataagtag tataaaatat
     1081 ccgaatagtg taaacaaaat atttacgggg tcggaaagcc atattatcat ttgactaata
     1141 atagagtagc ccaaaatcaa tatgattttg aaaatccaat gattcctaaa gtctataaaa
     1201 taggaagtca acgtataaag aaaaaaggct atgaaaatat tgattaatat tagaataaaa
     1261 tttatgcttg tagagattaa a
//
LOCUS       DAGSHT010000385         1245 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.391, whole genome shotgun sequence.
ACCESSION   DAGSHT010000385 DAGSHT010000000
VERSION     DAGSHT010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.391"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            155..652
                     /locus_tag="MJB72_003739"
     CDS             155..652
                     /locus_tag="MJB72_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY8264776.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            701..1225
                     /locus_tag="MJB72_003740"
     CDS             701..1225
                     /locus_tag="MJB72_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY8264777.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELKKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT          512 a          113 c          229 g          391 t
ORIGIN      
        1 aaacggtata gccataatgc tatgccgttt tgccatttat aataaaaata aatttattga
       61 cataatagta actaattact ataatataaa gtaattagtt actatttaaa attatcaaaa
      121 aattaaaatc taagataaag agaagtgtat aataatggat gcagaaagtt taatgaaaaa
      181 atatatggta gatgatgata gtagaaaaca agctatattt gcaagtatat ttgtaatgca
      241 aaataaactt caaactacat gtgataaatt agacccagaa ttaacaatga aacaatggtt
      301 acttttagct attgcggact ctgtagatga aactcttaca ttaaccaaac ttggagaatt
      361 aatggggtgt tcaagacaaa atgtaaaaaa actagctatt gctttagaaa agaaaggttt
      421 tatagaaatt gagcaaaatg aaaaagattc aagagctatc tgtcttgtga caaaagatag
      481 agtgcaagag tattctaaaa aagtgagagg tatgcaggaa aaagtattac agcttttatt
      541 tgaagatttt acagagaaag aagcggaaca gttatttttt ggaattatga aattaagtat
      601 aggaattgaa aatgtgaaaa attatgtaga aaataatgac gttggagaat aagcaagtat
      661 caaatcaact ttgtatgaca tcagagaggg gagaatttat atgaaaagtg tagtagtgat
      721 ttataagagt aagtatggtt caacaaaaaa atatgctaaa tggctttcag aattattgtc
      781 ttgtgatatt tttgaaacta agaatattaa tatagaaaag gttatgaagt atgatacgat
      841 tattttagga ggaggtcttt atgcttgtaa tatagcggga atttctttct taaaaaaaaa
      901 ttataaagaa ttgaagaaaa aaaatttagt agtatttggc gtaggagctg ctccatatga
      961 tgatgaagta ataaagaaat taagagaagt aaactttaag gatgaattaa acaaaattcc
     1021 ttgcttttat tgtcgtgggg catgggatga gacaaaaatg tcttttatag attatactct
     1081 ttgtagtttt tttaaaaaat ctatagctaa aaaagaacgt caaggtctta aaccagcaga
     1141 agcaccttta ttgtctggag aagcctttga ttggtgtaat aaagaaaatt taactccaat
     1201 acttgattat ataagagaac tttaagaggt atatatttgt aatta
//
LOCUS       DAGSHT010000386         1241 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.392, whole genome shotgun sequence.
ACCESSION   DAGSHT010000386 DAGSHT010000000
VERSION     DAGSHT010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.392"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..568
                     /locus_tag="MJB72_003741"
     CDS             <1..568
                     /locus_tag="MJB72_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264778.1"
                     /translation="ALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQFLI
                     YDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTSFFS
                     KKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTIDSIG
                     DLIFTCLIISTILIALAIVKENYKWREV"
     gene            571..>1241
                     /locus_tag="MJB72_003742"
     CDS             571..>1241
                     /locus_tag="MJB72_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264779.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFI"
BASE COUNT          455 a          143 c          160 g          483 t
ORIGIN      
        1 tgctttagtt tcactaggat ttcttgtaat aatttttata atgaacaata ttttgtcaaa
       61 ttatataagt gaatcaaagg gactttcaaa aagactttat tctatgccta taactagagt
      121 acaattctta atttatgact ttgtaggttt atggatttat tcatttatat tcttattatt
      181 atatgtttta ttctttagaa tacttggaat aacatttaaa ggtaattttg ccatattgct
      241 cctactttgt gctttatcat cttattttat gacttcaata tctacctttg taaccagctt
      301 ttttagcaaa aaatacggta cagcaatagt ttatgcatta ctctttttac agacaatttt
      361 tggaggtatc ttcagtatga taagtgatac atttactaag cttacaagtt tatctcctac
      421 ctatttaata ggagaattgt ttagtaatta tgaaacattt aaaactatag attcaatagg
      481 agatttaatt ttcacatgtt taattatctc tacaatatta attgcacttg ctattgtaaa
      541 ggaaaattat aaatggaggg aggtttaata atgattagat taactattgc aaatataaaa
      601 agatatctaa aaaatcatac tcttttaatt aatatggtta tgcttcctat tatactaatt
      661 ttttcactta atttttttat caacaactcg ggaaatcaat caatgtattt ttctccagta
      721 gcaattgtat ctgactcttc aggaaagtat gaacataagt taataaatag ttctaaatta
      781 aaagaaaatt catttagatt aaatgaacaa gataaggcta tgaatctatt aaaaaataat
      841 aaggtttctg ctgtattcgt tttagataaa aatttttcaa atagtataga taaattacaa
      901 cgacctgtag ttaaatgctt taaaattgaa aatggtggtg gtagtctttg ggcagaatca
      961 caaatagaat cttttattac taaatcgtta aaattgaaag tggataaaaa tatagatgat
     1021 aaattgacaa agacaaatat aattgataac aaaccagata agaataaagg ttcattttta
     1081 gtagtcttct tgatatgcta ttttatgtat attaatgcgg cacatcttgc ttctgattta
     1141 tttacattaa aaaaatcaaa tgtgctaaaa agacttatct ctacagataa taaagatata
     1201 aaaataattt tcagtatatt tttaggacta ttttttattc a
//
LOCUS       DAGSHT010000387         1233 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.393, whole genome shotgun sequence.
ACCESSION   DAGSHT010000387 DAGSHT010000000
VERSION     DAGSHT010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.393"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..523)
                     /locus_tag="MJB72_003743"
     CDS             complement(<1..523)
                     /locus_tag="MJB72_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264780.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGF"
     gene            complement(524..1231)
                     /locus_tag="MJB72_003744"
     CDS             complement(524..1231)
                     /locus_tag="MJB72_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264781.1"
                     /translation="MKGFKTNVLKNISLTVNEGDFISIMGPSGAGKTTLLNLMSTLDK
                     QTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMKLMDNIALPLALGKKK
                     SKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLITNPTVIFADEPTGAL
                     DSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMINDGNIKCKLNKGNSRK
                     EFYGKIMDMLASMGGEM"
BASE COUNT          405 a          200 c          125 g          503 t
ORIGIN      
        1 taaaaccttt tacagccatt attataaata tagaaataaa gattaaagta gcatatagta
       61 tagcttctat actaagtcca ttagatacct ttatattaaa tccagataac tttattatca
      121 ctacttgaaa aaatctagat gttattatcc ctataattat tcctgttata caagaactta
      181 ttcctataag catattttct acaaaatata tttttcctat agtatataaa tctaatccca
      241 taaaagacaa tattccaatt tctttagacc tattcttaaa aaatatattt gttgcatacc
      301 atataaagaa aaataaaaat actgatagta tcattattac agctactaac actgatagtg
      361 taaatttttt attttcatct tgtaacacat ttatagcatc accatatctt aataattcaa
      421 aattagttag cacaactact gaaaaaacca tagatataaa aaatgctaga taagttctta
      481 tgttactctt aaaattatta taagcaattc caagtatact cacttacatc tcacctccca
      541 tagaggctag catatccata atttttccat aaaattcttt tctactattt cctttgttta
      601 atttacactt tatatttcca tcatttatca tatatacttc atttgaataa cttgctgtta
      661 gagggtcatg tgtaatcatt ataatagtag cgttattttc tctgtttatt ttttctaatg
      721 attctaaaag ttcatatgct gatttagagt ctaaagcacc agttggctca tcagcaaata
      781 tgactgttgg atttgtaatt aatgacctag ctgctgcgcc tctttgtttt tgtcctcctg
      841 ataactgata tggatattta tctaaatgat tttctaatcc aaatttttta gctatagaaa
      901 ataccttagc ttctatctcc ttacttttct tttttcctaa tgctagagga agtgctatat
      961 tgtccattag tttcatattg tctaatagat tatagtcttg aaatataaac cctatttttt
     1021 ctcttcttaa ttttgatagt tcattatttt ttacttttga tatattaatt ccatctagta
     1081 ttatttctcc tgaagtttgt ttgtcaagtg tagacattaa atttagcaat gttgtctttc
     1141 ctgcaccaga tggacccatt attgatataa aatcaccttc attgactgta agagatatat
     1201 tttttaacac atttgtttta aatcctttta ttc
//
LOCUS       DAGSHT010000388         1206 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.394, whole genome shotgun sequence.
ACCESSION   DAGSHT010000388 DAGSHT010000000
VERSION     DAGSHT010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.394"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            308..682
                     /locus_tag="MJB72_003745"
     CDS             308..682
                     /locus_tag="MJB72_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY8264782.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            679..>1206
                     /locus_tag="MJB72_003746"
     CDS             679..>1206
                     /locus_tag="MJB72_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264783.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLD"
BASE COUNT          491 a          125 c          189 g          401 t
ORIGIN      
        1 aaatatgtat gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa cttttattta
       61 gctataagct aaactttatt atttttttaa tttagtattt tcaatttatt ggcaggattt
      121 ttgaacaata tttataaatt tttaaaaaaa atttatatct atttaatatg gcagcctttc
      181 ataaaataca ttacatttat taaatgtata atattggcaa ttagtttttt ctaaaatcag
      241 ttgacatatt atatatatat ttatataatg tgtatatact atatatacaa tttaaggagg
      301 tgtcttattg aatatagtta ttagtaattc gagtgggaag ccaatatatg aacagattac
      361 tacacaaatt aaaagtatga ttataagtgg agagttacca gaaggctcag ctcttccaag
      421 tatgagacta ttggcaaaag aacttagaat aagtgtaatt actacaaaac gagcatatag
      481 cgatttagaa cgagacggat ttattgagac agttactggt aaaggcagtt ttgtatctag
      541 taaaaacatg gattttgtca aagaagaaca atttcgttta attgaagaat acctacaaaa
      601 agcagttgat acagcaaaaa gtagtgatat tacatatgac gaattaaaag atattttatt
      661 tttattatat aaaggagaat gataagtatg gatgcaataa ctataaaaaa tttaaataaa
      721 acttataaag attttagcct acaagatata tctttttcag taccaaaagg gagtgtaatg
      781 ggctttgtag gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat tttaaattta
      841 atatcttatg atagtggaaa tatagaaatc tttggtcttg atagtaaaaa aaatgaaaaa
      901 gaaattaaag agcaaattgg ggttgtattt gagggaagca actttcatga aaatttaaac
      961 actgaccatg tttcaaaaat catgagtaaa atctataaaa attggaatga cacattattt
     1021 aaagactact taaaaaaatt gagagtacca gacaataaat taattaagga gttttcaaag
     1081 gggaataaga tgaaacttag tattgctgta gccttatctc ataaaccaaa attattaata
     1141 ctagatgagg caactagttc acttgaccct atcgttcgtg aagaaatact agatatattt
     1201 ttagat
//
LOCUS       DAGSHT010000389         1158 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.396, whole genome shotgun sequence.
ACCESSION   DAGSHT010000389 DAGSHT010000000
VERSION     DAGSHT010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.396"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     repeat_region   546..1099
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=546..574
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT          446 a          113 c          177 g          422 t
ORIGIN      
        1 tacagagaga gcataattta ttttaagtat ctctaaaaaa attaaatatc tagggtttta
       61 tttgacgtgc tcttttttag atagtaagct ttaaaatata gatattaatt atatgaatat
      121 aataaaaaga gtactaatga gttacactag tactttataa ctatttttac atgttttaac
      181 tgtataaaac agcgggtata gttcacacag gcaggagtga ctttagtttt gaactaaaaa
      241 tcaagttcaa aaagaataaa cattagtatt tgaacttcac tctacgtcta aatagattgt
      301 agttcttctt gcttttatta taccacaaat tgatatagat attcaaaaat aatatattta
      361 tgatataata aaaatgtaaa tagttttgca gtgagcgata tttgttacaa agtagagctt
      421 aacgcttgaa atataaggtg ttgagggtat atgataagct ttatcatttg cactactcat
      481 ggttcactgc aaatttaaga gagttgcata tgtgtaagta ttgaaaatgc ccagtttatt
      541 tgggggtttt atattaacta agtggtatgt aaatagagag ataaggcaaa taatctgtaa
      601 caactccaaa gttttatatt aactaagtgg tatgtaaatt ctgaatacgg aacataaata
      661 gcaacagtac cagaatgttt tatattagct aagtggtatg taaataatga tttattaaaa
      721 atttttattt gttttgatat tgttttatat taactaagtg gtatgtaaat atagtccttt
      781 tctaaattta gaatcaagtt ttggagtgtt ttatattaac taagtggtat gtaaatagag
      841 taaaacaata gtacaagaac aaataaatac atgttttata ttaactaagt ggtatgtaaa
      901 tactttctgt agctctatag ataaataagg tgctaaagtt ttatattaac taagtggtat
      961 gtaaattaac tttataaaac ccagtaattg caacactttt atagttttat attaactaag
     1021 tggtatataa atagtataat gttgaaaagt tagagagtac aatcaagaaa ttttacatta
     1081 actaaaatgt atagaataaa acatgtattt atacttaaat tctgtaccta tataaaaaag
     1141 tgaactctgt caacaaag
//
LOCUS       DAGSHT010000390         1158 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.397, whole genome shotgun sequence.
ACCESSION   DAGSHT010000390 DAGSHT010000000
VERSION     DAGSHT010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.397"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            94..804
                     /gene="rgaR"
                     /locus_tag="MJB72_003747"
     CDS             94..804
                     /gene="rgaR"
                     /locus_tag="MJB72_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBY8264784.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          456 a           96 c          189 g          417 t
ORIGIN      
        1 ttataaagaa atttaaattt gaaagctagt gagtacagtt tggaaattga aataaagtaa
       61 actaaatgat attttagatt tggaggtgat attatgttca gggtagttat atgtgatgac
      121 gaaaaaatac aaagaagcat actaaaagag tttacagaaa aacttttgtc tgaaagatgt
      181 ctaaattatc aaatactgga attttcctgt ggagaagatt taatatccaa atacccagaa
      241 aaaattgata ttatattttt agatatccaa atgaaagata tcaatggaat tgaggtggct
      301 aaaaaaataa gaaagtttga tgaaaaagta gaaattatat ttacaacggc tttttcagag
      361 tatgcccccc aaggttatga ggttcgtgct tatagatatt tagtaaagcc tatagagtat
      421 agtaactttg caatgggtgt aaatctttgt attgataacc ttcaaagaaa gaaggagcgt
      481 tatatagttt taaatagtaa aaaaggattt aatagaatac tgatagattc tattttatat
      541 gttgaaactg taaagagaga tttagtcgta catacaatag gtagagatta taaagctaat
      601 atgagcatga aacaagcaga aaaattactg tgtgaaaatg gcttttttag atgtcataca
      661 agttttattg ttaatttaag aagaatagaa gatcttaggg ataatatgat tactattaat
      721 gaacaattta tacctataag taagtataga cttaaagatt taaaagtagc attaacaagc
      781 ttgttatgtg atataatgta ttaattttaa aaaggtaatt gaaaatatcg taaaaaagat
      841 ttaatgtcat cacagggcta ttgattgaaa ctgtcttaat atataaaatg ttatttatat
      901 taagacagtt ttattatgca aaatagcagt ttatagattt ttaatatggt ttaacatgta
      961 tatgtagagg ttccattata gtagcatgat tattaattag tagcttgatt atcaatatac
     1021 tattattgat ttttatatgc attatatgta tttttagaat atgattacct ttttaaaata
     1081 atgactattt ataaaactct atagatattt gtttcattta gctatgcata ctttaattaa
     1141 ttatattaat atttaatg
//
LOCUS       DAGSHT010000391         1148 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.398, whole genome shotgun sequence.
ACCESSION   DAGSHT010000391 DAGSHT010000000
VERSION     DAGSHT010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.398"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            339..998
                     /locus_tag="MJB72_003748"
     CDS             339..998
                     /locus_tag="MJB72_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264785.1"
                     /translation="MIIEVSNLSKVVDLLNTELTILRNIDLYIEEGEFVSIMGASGSG
                     KSSLISILSGLDKNYTGNVKVCGENLSKLTDDELAEYRNRKIGIVFQSFNLITSMSVE
                     ENIKLPLIFSDNKDYKIVDKMIKKVGLTSKTKNNIKQLSGGEKQRVAISRALVCSPNI
                     LFADEPTGSLDSTNSHAIMKLFKNINNEYGTTIVMVTHDIQMAEYSDRIISIKDGTII
                     K"
BASE COUNT          448 a          108 c          181 g          411 t
ORIGIN      
        1 tataaatata taaattttgg ggagagattc tgtatatcta agctaaaact tagtattaaa
       61 ttaaatccca cttccgaatt attgatgata ttatattttt tagatttatc attgaaaaag
      121 tttatttttt ataattttac taacttttgg tcaagcaatc ttatgtataa taagaaatat
      181 aatgacctat taagatttgt acagttttag aaaatatatc attctcaaaa aagcttgctt
      241 gtataagttt ctctaatatt ttgtgtccta tatactctgt tttaattaat aatcaatctt
      301 aaaagttttg tatgaaagga aataatggta tgaaaaaaat gataattgaa gtttcaaact
      361 tatctaaagt tgtagacttg ttaaacacag aacttactat actaaggaat atagatttat
      421 acatagaaga aggagaattt gtttctataa tgggtgctag tggtagtggg aaaagctctc
      481 taataagtat attaagtggt cttgataaaa actatactgg aaacgtgaaa gtttgtggtg
      541 agaatttatc gaaattaact gatgatgaac ttgctgaata tagaaataga aagataggga
      601 ttgtatttca atcatttaac ttaattacat caatgtctgt tgaagaaaat ataaaattgc
      661 cattgatttt ttctgataat aaagattata aaatagtaga taagatgatt aaaaaggttg
      721 ggttgacatc aaaaacaaaa aataatatta aacaattatc aggtggtgag aagcaaagag
      781 ttgctatttc aagagctcta gtttgttctc caaatatact ctttgctgat gaaccaactg
      841 gatcattaga tagtactaat agtcatgcta taatgaaatt gtttaagaat ataaacaatg
      901 aatatggtac tacaatagta atggttactc atgatattca aatggcagaa tatagtgata
      961 ggattataag tattaaagat ggaactatta taaaataggt ggtgatacat tgatatttca
     1021 tgaagtttat aaaatatata gtaagaatag tttgaagaat aataaattat taaaaatctc
     1081 ttgtataata tctattgtat ttgccacagc aatattactt tttacaagag tgttaacgga
     1141 tacatatt
//
LOCUS       DAGSHT010000392         1109 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.399, whole genome shotgun sequence.
ACCESSION   DAGSHT010000392 DAGSHT010000000
VERSION     DAGSHT010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.399"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(37..951)
                     /locus_tag="MJB72_003749"
     CDS             complement(37..951)
                     /locus_tag="MJB72_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBY8264786.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT          353 a          163 c          138 g          455 t
ORIGIN      
        1 taaatatgca aagattattt taaaagtaat ttttaattac atgtttttat aactctccaa
       61 tattctaata taatgatttg cctctcttaa aacatggtct gctaaaagtg gtaatattat
      121 agattttatc ttacactgtt ctattcctga tgctcctgct tctttaaaat ctttaaactc
      181 aacagtttca tttagagttt cttctgtaat gttcttgata ttagaatcag tcatttcgtt
      241 tgttttttca attaattcat taaattttat agcaaaatca tttgaagtat ttattagttc
      301 accttctgag gggtctagta atcctctcat aaacagagca tgttccatca taatatggtc
      361 ccagaataat tctatttctt tagcgttttt tgactcaata tctattttat tttcaaggtc
      421 aactacataa gaacgatata aatttgcttc atgtattata tgttcaagaa gtagagggta
      481 gtttgatgta aatatagtac atgatagtac accatctaag actctttctt taaaattaat
      541 aagcccatca agtaatctta ttgcatcaga gttaagttga gctacatagt tcactaagtc
      601 ttgaccaact tgtgggttta caccactttg tagatttaat tctcttgtag tgatgttttt
      661 gtttatttct atccctgtaa actcttctgt tttttgttct gcaactgatg tgagagtagt
      721 tacaagctct tctgaatata atatatcagg tctaattata ccattactag cactaacagt
      781 gtatgataat aaatcttcaa attgcttttt atagtggtca gcttccatag caagattata
      841 atttttattt gtgaatcctg cttctaaaaa aagagcatgc tctttcataa ttcttgaaaa
      901 aaataaatgt aattctagtg ataaaataac atatttttga ttatctatca taatttcctc
      961 cccttagaaa gtatcctatt tataatactt tattcgtaat atttaaattt atgtatatta
     1021 taaataaatt aactaaattc aattgctact aatatcaaca gtttttatta agttatgggc
     1081 gaaaagatta tcttactgtg tttcttatt
//
LOCUS       DAGSHT010000393         1093 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.400, whole genome shotgun sequence.
ACCESSION   DAGSHT010000393 DAGSHT010000000
VERSION     DAGSHT010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.400"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            90..1093
                     /locus_tag="MJB72_003750"
                     /pseudo
     CDS             90..1093
                     /locus_tag="MJB72_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
BASE COUNT          484 a          136 c          152 g          321 t
ORIGIN      
        1 aaaatttgca tttaataaag taataagaac aaaaaatcaa aacgaaattt cattagaaaa
       61 atttaatgag tttaaagaaa ctatacaaga caaattatta ccatacaaca gataattcaa
      121 ctgctgttca aaatagcaat ggataatcat gaaatactgg attaagtata aacaaaggag
      181 aatctgcata tataaatgca aatattagat actacaacac aggtactgca cctatgtaca
      241 aagtgacacc aacaacaaat ttagtgttag atggagatgc attatcaact atcaaagcac
      301 aagaaaatca aattggcaat aatctatctc ctggaaatac ttatctcaaa aaaggatttt
      361 cacctatggc tcttaacaca atgtatcaat ttagttctag actgattcct ataaattata
      421 attaattaaa aaaattagat gatggaaagc aaattaaatt agaaacaaca caagtaagtg
      481 gaaattttgg tacaaaaaat ggttctggac aaatagtaac agaatgaaat agttggtaag
      541 acgatatagg tcaaattgac agtatttctg tatctattat attagataca gaaaatgaat
      601 cttacaaaag aagaattact gctaaaagtt tacaggtccc agaagataaa acacctgaac
      661 ttacaattgg agaagcaatt gaaaaaactt ttggcactac taaaaaagat ggtttgttat
      721 attttaataa tataccaata gatgaaagtt gtgttgaact catatttgat gataatatag
      781 gcaataagat taaagacagt ttaaaaactt tgtctgataa acaaatatgt aatgttaaac
      841 ttgaaagagg aatgaatata cttataaaaa caccaactta ctttactaat tttgatgatt
      901 acaataatta ccctagtata tggagtaatg ttaatactac aaatcaagat ggtttaaaag
      961 gctcagcaaa tagattaaat ggtgagacga agattaaaat acctatgtct gaactaaaac
     1021 cttataaacg ttatgttttt agtaaaattc aaaaaatcct ttaacatata attcaataat
     1081 tgtaaatata aaa
//
LOCUS       DAGSHT010000394         1077 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.401, whole genome shotgun sequence.
ACCESSION   DAGSHT010000394 DAGSHT010000000
VERSION     DAGSHT010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.401"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            299..865
                     /locus_tag="MJB72_003751"
     CDS             299..865
                     /locus_tag="MJB72_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBY8264787.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          455 a           78 c          167 g          377 t
ORIGIN      
        1 aaacattttt aaatctaatt atatataggt gaaataaaaa attaattata agtaaggcat
       61 ttagaataaa attttaaaaa aattataatt aaaaaaataa tgaaaatata taatattagt
      121 ttagattata aaaaataaat atacaaatac ttataaatac aagttatata caagatttat
      181 agtagagtaa taatcttata aaataattta ataaatataa attaaattat atgtgtatat
      241 attgtacttt aatagtgata taataataaa aaaattttta aagagggtga tattacatat
      301 gagaaaactt gaagagatat tagaatataa taaatcattt gtagagaaca aagaatatga
      361 acaatatgtg acatcaaaac atccaaataa aaaaatagta gtcttatctt gtatggatac
      421 aagattaact gagcttttgc ctaaggctat gaatttgaaa aatggagatg tgaaactaat
      481 aaaaaatgca ggagctacta taatgcatcc ttttggaagt attatgagaa gtgttttagt
      541 tgcaatttac gaatttgatg tagatgaggt tatggttgta gggcatcatg gatgtggtat
      601 gtgtaatgtt gatacggata agttacttgg taaaatttta gatagaggaa tatctaatga
      661 tgtaatactt acacttcgta gtgctggaat tgatacaaaa caatggttac atggattcga
      721 ttctgcagaa gaatctgtaa aagatagtat tgatttaata aaagggcatc ctttaattcc
      781 agatggtatt attgtacatg gtctaattat atcacctgaa actgggaagt tagacgttgt
      841 tgtaaatgga tatgaagata agtagagtat cataatgtta tataatcaag atactgttga
      901 atatgatgtt gtttcaaaat ttattttggg acaacttttt tatttgaaaa tatatgaaaa
      961 ggtttatttt aagctttgca ataaaaaata taagtagctg actaattaaa aatcttacta
     1021 ttatttatta aaattataac tatatgatat aaaaatattt aataaatgaa atttata
//
LOCUS       DAGSHT010000395         1059 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.402, whole genome shotgun sequence.
ACCESSION   DAGSHT010000395 DAGSHT010000000
VERSION     DAGSHT010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.402"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..951)
                     /locus_tag="MJB72_003752"
     CDS             complement(<1..951)
                     /locus_tag="MJB72_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264788.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPL"
BASE COUNT          379 a          143 c          115 g          422 t
ORIGIN      
        1 taaagggatt attccatttt ttaattgata ggttaatttt aaatcgttta taagtgtatt
       61 tgtatattct atgttcttta acatgattga agcatattta tatacttttt caggtgaata
      121 attagtacta ctatctgtaa gaagctctgc ataccctttt attggtgaaa gtggagtttt
      181 taaatcatga gttatatttg aaatccactc ttctctcata atatcagttt tattttgtat
      241 ctcatcactt ttatgaattt cttcatttaa aatatttaaa ctattatata tatcagcaaa
      301 aacaccttta atattaactg gaagatagct tcgttttgaa atatcactaa cagcagatgt
      361 aatatttgat atgcactttg ttacccagta accataagca attccagaaa ccaaaattat
      421 taaaaatata atccctatga ttgaaaatac aattgactta ccacctataa atttacctcc
      481 atcaaaaaac atattcactt tagatatatt taatggaaaa tatataatat aaatatagtc
      541 atttttatta tttttaattg tattaaagta tacagttcca ttacttgaat tattttggtt
      601 agctaatgct aataattcag aattagaata ataatcttta tgctcttttg gttctaaaaa
      661 cccataaact ctatttccac tagaatctaa aatttgcaac cacaattgat tttctcgaag
      721 tttttctaat ccagactgtt ttacttgtgg cttattatct ataaatacga tttgttcatt
      781 aaagttttct gtaaattttt tcggccagtc actacgaata aattccccat ttggtttctg
      841 aatagtaata agtgataaga aaatacctat tactgtcagg attaagcaaa taaaaatcat
      901 gaaaaaaata atgtatgaat gaaatactgt tttataacta ggtttatgca tttaatcaac
      961 tcttttctta aacttttatt ttagtttgat tgttgcttat ataaaataga caataattat
     1021 tgatatgtaa aaatttattt aaaaatacaa ctatctaca
//
LOCUS       DAGSHT010000396         1052 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.403, whole genome shotgun sequence.
ACCESSION   DAGSHT010000396 DAGSHT010000000
VERSION     DAGSHT010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.403"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(71..850)
                     /locus_tag="MJB72_003753"
     CDS             complement(71..850)
                     /locus_tag="MJB72_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBY8264789.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT          418 a          178 c           92 g          364 t
ORIGIN      
        1 cagttttatc aatgttatac tttaatcttt aattaaagcc atattttatt taaatatata
       61 actatatatc ttagtacata gtataagatt tttctttatc taatagtata tcctctacat
      121 ctgggttttc ttttactaat tgtctataaa aattacaatt agtaacacct acatatggaa
      181 ttagccatag tagacctatt ccaagagtag ctattgaaag tatataccat cctataaaac
      241 taaagtttag tacaaataat ttaagtacat ttcctttcat catatctaaa ctagcaccca
      301 ttgatttaaa tacacccata ttatccttgt cttcacaaat taagaaagta gcttgagaaa
      361 catataatag caatatataa accggtaaca taagaacaag tattataaga actagagtta
      421 ttccagtaga aataacagtc cctattccaa cattatcact tgataaatat tcaattgctc
      481 cactagtaag taaaactcca aatgataaaa acgctcctat aattgtgatt ataaaagaat
      541 aaatataaac aataattgat tcaaataaat aaaaaccaaa acttcttaaa taaattctcc
      601 cacttacaaa gatatctcct atcttgactt tttcatcttt agtaattttc aagaacatac
      661 atgtaaatcc tgctgaaacg caaaaagcta ttacagccac tacaatagta gtcaatggtt
      721 caaaaggtaa tcctacaatt gatattaata ctatcaatgc aatatatact gccatagaaa
      781 ttgcacatat cccccagttt ccagataact gagctttaga tactcgtttt aattcttgcc
      841 ttgataccat aaaaaacacc cctttcattt tataaaaaaa cttttaaaaa tataaataat
      901 cttttaacgt ataatattaa tatatcatat tttcgactat ataagataca attattcacc
      961 atgttttatc aagatatacc ataattgtat tactagtttt atatagtcaa cattaccaaa
     1021 tacatcaaat aaatttgatt aattattcaa ca
//
LOCUS       DAGSHT010000397         1047 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.404, whole genome shotgun sequence.
ACCESSION   DAGSHT010000397 DAGSHT010000000
VERSION     DAGSHT010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.404"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..982
                     /gene="mltG"
                     /locus_tag="MJB72_003754"
     CDS             <1..982
                     /gene="mltG"
                     /locus_tag="MJB72_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBY8264790.1"
                     /translation="DKNNKKDVIIDVPSGASVGKISDILYENKLIKNELLFKLLVKVS
                     NKAPSIKSGTYLLNQSYSNNDIISLLVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNL
                     GDKATFENLIKKPQEFYDKFPYLKEDGITSLEGFLYPETYYFNSKKQSEEDILSEMLK
                     VFDSKYTDKFKKKQKELNMTLQEVMEMASIIEKEAVLDKDRPIIASVFYNRLKVGMPL
                     QSDATIQYIFEERKKIVTYDDLKIDSPYNSYKNKGLPPTPISNPGIKSIEAALYPEKT
                     DYLYFVAKIDGGNNYSTNYQDHLKYVKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT          463 a          118 c          161 g          305 t
ORIGIN      
        1 tgataaaaat aacaaaaaag atgtaattat cgacgttcca agtggagcat ctgttggaaa
       61 aatatctgac atattatatg aaaataaatt aataaagaat gaactattat ttaaactttt
      121 ggtaaaggtt agcaataaag ctccgtctat aaaatcagga acatacttac ttaatcaatc
      181 gtattcaaac aatgatataa taagtctttt agtttcaggt aaaatatatc aggatggtat
      241 aaaagtgact attccagagg gagctacatc taaagaaatt atagcaatgt tagtaagtaa
      301 aaatttagga gataaagcta cttttgaaaa tttaataaaa aaacctcaag aattttatga
      361 taaatttcca tatttgaagg aagatggaat aacctcttta gaagggttct tgtatccaga
      421 aacttattat tttaattcaa agaaacaatc agaagaagat attttaagcg aaatgttaaa
      481 agtatttgac agtaaataca cggacaagtt taaaaagaaa caaaaagaat taaatatgac
      541 tcttcaagaa gtaatggaaa tggcatcaat aattgagaaa gaagctgttc ttgacaaaga
      601 cagacctata atagcaagtg tgttttataa tagacttaag gtaggtatgc ctcttcaatc
      661 agatgctaca atacaatata tttttgaaga gagaaagaaa attgtaacat atgatgattt
      721 aaaaattgat tcaccatata atagttataa aaataaggga ttacctccta caccaatatc
      781 aaatcctggt ataaaatcta ttgaagcagc attatatcca gagaaaacag actatttata
      841 ttttgtagct aagattgatg gtggaaataa ttatagtaca aactatcaag accatttaaa
      901 atacgttaaa gaatataaag aagcaaggga taaacaatca aaagatacaa aagcaacaaa
      961 taaagaaaat acaaaaaaat aaaccatgat tataaaaagt ctaacagggt aagaataatg
     1021 ttgggctttt ttattgaaaa ttgttat
//
LOCUS       DAGSHT010000398         1043 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.405, whole genome shotgun sequence.
ACCESSION   DAGSHT010000398 DAGSHT010000000
VERSION     DAGSHT010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.405"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            123..983
                     /locus_tag="MJB72_003755"
     CDS             123..983
                     /locus_tag="MJB72_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY8264791.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT          383 a          126 c          172 g          362 t
ORIGIN      
        1 aattcaaaaa tattaagaat aatgaaatac ttgaaattgc ttatatcaag aaattttata
       61 taatataaac tggtcaaata tgttataata ttaaattgtg acaagttcat ggaggtgttt
      121 atatgaaaaa acaaaagtcg ccaattttac ttgaaatgct tggactattt tttggttgta
      181 tatcaatgag tattggtata aatatgtttt taaaaccaca tacaattgct cctggaggac
      241 ttagtggtct atcattagta ctaaacaagg ttacaggact tccagtatca gctatcatgt
      301 taattatagg tgtcccatta gtaatacttg cttttagaat tatgggtaca aaaaattcat
      361 taaaaacttt atttggaaca gttgtatttt cagctatagt tcaattaaca gacccattat
      421 caaaactaag atttactaat gacttattat tgtcttctat tgcaggagga atcctagttg
      481 gtataggttt aggaataatg tttaaatcag atgcttctac tggaggaaca gacttaattg
      541 cactcatact tagtaagaaa ttcccaggaa taaaagctac aaaattcatg tcatgcctag
      601 atggaatggt tgttatatct tctggtatag taaatcgtag tttagaaact gctctttact
      661 ctggtatagc actttgtgta ttaataaaaa tagcagatat gataatggaa ggatttgacc
      721 attcaaaagc attttttatt atctctgatg agcctgaaag tttaagacaa gctataactg
      781 aagaacttga tagaggtctt actattcttg atggtaaagg tggatacact agagaaaata
      841 aagaagttct attagtcgta gtttctaaaa aacaagaact ttatctaaag agattagtta
      901 aaagaactga ccctactgca tttgttattg ttacagatgt acatgaggtt cttggagaag
      961 gatttaaaaa tttagagaac taataaaaat taatatttta agtatatata ctattttaat
     1021 taaaatacga tttattcaaa aag
//
LOCUS       DAGSHT010000399         1042 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.406, whole genome shotgun sequence.
ACCESSION   DAGSHT010000399 DAGSHT010000000
VERSION     DAGSHT010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.406"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            33..608
                     /locus_tag="MJB72_003756"
     CDS             33..608
                     /locus_tag="MJB72_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264792.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            706..>1042
                     /locus_tag="MJB72_003757"
     CDS             706..>1042
                     /locus_tag="MJB72_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 family protein"
                     /protein_id="HBY8264793.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYL"
BASE COUNT          424 a           93 c          199 g          326 t
ORIGIN      
        1 gctaatatat caaaataata aggggggaat ttatgaagct taaagaagca ttgattacat
       61 gtgttgtatg tagtgtatgt ttaactccat tattggtaaa tggagaaaca aaaatagatg
      121 cagatcattg taaagaatct atagtaaata tagatactat agtaaagtca ggtaaaagct
      181 ttaattccaa gtataatcaa ggtatggaag attataaaaa tagtgatgag tataagttga
      241 aagtacaaaa ggagaaacta caaaatcagt taggtattaa aataaagaag ttagtaccta
      301 taacttttga agttagttat tacacaagtc ttaatcaaga aaatggatat ggagcgataa
      361 ctgctacagg agatgattta gaagatggtt ttgtagcaaa caatcactta agttttggaa
      421 ctaagattat tgtagatgga aaattaaaaa ctgttatgga tagggggtct agtaagtatt
      481 ttagtaattc taatgcaata gatgtatttg tgcccaaatg gagtaatgaa agtgaatcag
      541 agtactatga tagagtcaat aatatgggaa gacattacaa aaaaggctac attattgttg
      601 aagaatgatg ttctttgaag taatatgtat tgattgtagt atttaatata tatataataa
      661 atataaaact attagtacaa tgagatatat tactaggagg gcaatatgga acaatcgttt
      721 ataaatggaa aagtaaactt attaatagaa aattacaaag cattaaatga agttaaaggt
      781 tcttggcaaa tgggattgat acaacatagt tgtgcacttg catttaccct taagaacaag
      841 agaataagtc ctagattagt ggaagaacga atagagctta ttaaaaaaaa tactggatta
      901 ttctctaatt ttagaggata taatatgttt tatatggcaa cactactttc gtttgaatca
      961 aatccagaaa gtagttttaa gatgatttta gatatataca aagaacttaa aagtgaaaag
     1021 ttttggggcg atacatattt ac
//
LOCUS       DAGSHT010000400         1040 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.407, whole genome shotgun sequence.
ACCESSION   DAGSHT010000400 DAGSHT010000000
VERSION     DAGSHT010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.407"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..601
                     /locus_tag="MJB72_003758"
     CDS             <1..601
                     /locus_tag="MJB72_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBY8264794.1"
                     /translation="SGLSPFQIALMVTLVFGGSSQFIAASMISTGASVTSIVLTTFIV
                     NLRHFLMSSNLNMYIKNKSPKFILPFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFF
                     CLISSVLANFIGAFLGESISIDSSISGFILTSMFIALIVSQIKNKIYIVVFISSIIIS
                     VILYALFKSNLVIIVASILASLIGFFIEESYCKKEESYE"
     gene            594..917
                     /locus_tag="MJB72_003759"
     CDS             594..917
                     /locus_tag="MJB72_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBY8264795.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
BASE COUNT          343 a          119 c          135 g          443 t
ORIGIN      
        1 atctggtctt tcaccttttc aaatagcact aatggtaact ttagtatttg gaggaagttc
       61 tcaattcatt gcagcttcaa tgatttctac tggtgcaagt gtaacatcta ttgttttgac
      121 tacatttata gttaacttaa gacattttct aatgagttct aatttgaaca tgtacataaa
      181 aaataaaagt ccaaaattta tattgccatt ttgtcatact ataacagatg aaacttttgc
      241 agttaattat gaaaaattta ctgctggaaa ttggtctgat agaaatgcta tttatcttaa
      301 tttcttctgc ttaatttcat cagttttagc taattttatt ggagcatttt taggagaaag
      361 tatatctata gacagttcta tttctggctt catattgact tctatgttta tagctttaat
      421 agtttctcaa ataaaaaata agatttatat tgtagtattt atatcatcaa tcattatttc
      481 tgttatttta tatgcattat tcaaaagcaa tttagtaata atagttgcat caattttagc
      541 atctttaatt ggtttcttta tagaagaatc ttactgtaaa aaggaggaat cttatgaata
      601 ataattatat attttttatt ataattggaa tgtacattgt tacatatcta ccaagagtgc
      661 ttcctatgtt aattttttca aaaaaagaga tgcctgattt tttgaaaaaa atcatgaaat
      721 ttatacctgt agcaatactt acttccttaa ctgctaaaga tgtatttttt aatggagata
      781 gtctatattt atctatagct aaccctaaaa ttatatcttt tatgattgta cttcttgttg
      841 catataagtt taaatctatt ggtatttcta ttattacagg agtgatatct atatatttat
      901 ttggaattat cttgtgatat ttggataatg ttttattgta taagttattt taaaactatg
      961 tgtacaattt taatttaagt tgttatatta ttgattttaa ataaaaaaca gagtgactta
     1021 aagttactct gtttttactc
//
LOCUS       DAGSHT010000401         1039 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.408, whole genome shotgun sequence.
ACCESSION   DAGSHT010000401 DAGSHT010000000
VERSION     DAGSHT010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.408"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            66..710
                     /locus_tag="MJB72_003760"
     CDS             66..710
                     /locus_tag="MJB72_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8264796.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            924..>1039
                     /locus_tag="MJB72_003761"
     CDS             924..>1039
                     /locus_tag="MJB72_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBY8264797.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQ"
BASE COUNT          459 a           86 c          154 g          340 t
ORIGIN      
        1 taaataacaa aagaatcata tcatataata atataaaacc aatcataata aagcgggtga
       61 aaataatgga acaaaataat aaggttggga atttcggtga tttgttgaag attttattag
      121 atgaaaaaga attatcaatg ggagaactaa gtaaaaaaag tggaattgat aaatctacta
      181 tatctcgtat agctaataat aaacaaaagc caaatataaa tcatttagaa aagatggcaa
      241 tacatttaaa tattaaccta gaagaactgt tgaaagcttc aggatatgaa tttagaaaca
      301 gcaataatca aatatttaat gcagatagtg attttagtaa ctttgatgat attcttggat
      361 ttgcaaattt aatcaatgat aagaacttta atggaaatat tgaaaaagag ttaagtaagt
      421 gtaagttata tgtccaaaca gatgaaggta aaaaattact ttttgataat tttaataaaa
      481 aaatagattc tattgaaaaa caaggtcaat ttacagatag attaagacaa atgtatgctg
      541 atttttgtac agatggactt acaataaaga aatatttatt gattggaagt atgctacttt
      601 actttgttat atcaacagat gtgattccag acttcgtgtt cccaatagga tttatggatg
      661 atttagtagc attaaatata gttacaaaat tacttaaaaa tgataagtag gttttagaat
      721 catatttaat atataaataa atcaaagtat ataaaattta ttatttttta tatgaaacaa
      781 attaaaagca caatgaagta gattaaaaat ttaaaactaa gaatctaata tatattttgt
      841 aactatttaa gtatataaaa taaaataata tccaaactaa tacaaaatag taataattgt
      901 tttgtactgg aggatatata tttatgaatt gtaaatggat atctaaaata gatgagagaa
      961 aaaaatgtca tagagaagca gattcttctg gatattgtat ctttcataaa gaaaataagt
     1021 ctgatgaaga gatacagtt
//
LOCUS       DAGSHT010000402          998 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.409, whole genome shotgun sequence.
ACCESSION   DAGSHT010000402 DAGSHT010000000
VERSION     DAGSHT010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.409"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..962
                     /locus_tag="MJB72_003762"
     CDS             <1..962
                     /locus_tag="MJB72_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264798.1"
                     /translation="DSKNTNNLIDVSSGSDLIAHVGFEMNEESKDINFVIDEKKEDLE
                     KGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDDYYAKILASKRYKKYLYVSVLYA
                     TSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLSNYFSEKYQNIENIGVSYASKYE
                     DKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESIMELKTDSKMALAKAKLEANKLY
                     MVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKIVAYTSR
                     LNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNSMLEFEIK"
BASE COUNT          455 a           76 c          161 g          306 t
ORIGIN      
        1 aggatagtaa gaacacaaat aatttaattg atgtatctag tggttcagat ttaattgctc
       61 atgttggatt tgaaatgaat gaggagtcaa aagatataaa ctttgtgata gatgagaaaa
      121 aagaagattt agaaaaaggt gtatttccat cagaaggtag tgataaagaa attgaatatg
      181 aaaaattaga tatgaataat tttacaagga caagatataa agtagataaa agtacagatg
      241 attattatgc aaagatacta gcatcaaaaa gatataaaaa atacttatat gtatctgtac
      301 tttatgctac aagtgattca tcagaaaata acaaagaata ttcaagtact atagatataa
      361 taaaaattaa tccagaaaat aaaaaaatca agactataaa aagtataaat ttaagtaatt
      421 atttttccga aaaataccaa aacatagaga acataggtgt aagttatgct agcaaatatg
      481 aggataagat gtatatttta gtaaatgtac taaatacaaa cataggtgat aatagtaatt
      541 ctaaatttca tttacttgag tataatcttg aagaaaataa ggaaagcata atggaattaa
      601 aaactgatag caaaatggct ttagcaaaag caaagttaga agctaataaa ttatatatgg
      661 taatgtcgaa tgattatgat aatagttcaa ttacagagtt aaatttactt acctataata
      721 tattagataa aagcttagat gaagataaat atgttttaga taatagagaa aaaagaggaa
      781 ttagaagtat tgaaattgat tcaaataaaa tcgttgcata tacatcaagg ctaaactcta
      841 tattatcttg ggatgataaa tttatgctat atataataaa taaaaattct aaggaaattg
      901 tatatgaagg agaattacaa tttaacagtt caaatagtat gttagaattt gaaataaagt
      961 aggtgagttg atgaaagatt taataagatt attcgaat
//
LOCUS       DAGSHT010000403          957 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.410, whole genome shotgun sequence.
ACCESSION   DAGSHT010000403 DAGSHT010000000
VERSION     DAGSHT010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.410"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            66..719
                     /locus_tag="MJB72_003763"
     CDS             66..719
                     /locus_tag="MJB72_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY8264799.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            795..>957
                     /locus_tag="MJB72_003764"
     CDS             795..>957
                     /locus_tag="MJB72_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264800.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINL"
BASE COUNT          414 a           83 c          180 g          280 t
ORIGIN      
        1 caacaaaaga atagaatggg tatatttgtg aaataataat ataaaataat cttaggagtg
       61 agcttatgaa gaaaaaatta ttatatataa atgtaaattc aaaacctgaa gacttatctt
      121 caagtaaaac tgtagcaaga aaatttataa ataaatttat ggaaaaaaat aaagattttg
      181 aggttgaaga gatagacctt tataaagaac atataccaag acttgagtat cagtactttg
      241 aaaaaagaaa tagcatagtt agtgaagaaa atgctaaaaa tttagacgat aaggatagaa
      301 aagagcttac aaagattaga aatctttgtg accaatttgt gagtgcaagt gtatatgtaa
      361 tagcagcacc tatgtggagt ttatcttttc cagcaccact taaagagtat atagattgta
      421 taattcaaga tggaaaaact atatcttttg aaggcaatga taaaccacaa ggaattttaa
      481 atgatattga tagaagtatg gtatatatac aatcttctgg tggacacata ccatgggtgt
      541 tgaagccagt tatgaataaa gggttaaatt atgtggaaag tattatgaaa tttataggaa
      601 ttaaaaaatt tgatgaattg ttagtagatg gaactggaac tagtgaagaa gaaagacaag
      661 ctgcaataga aaaagcatct gaaaaaatag atggtataat agatgggatg aaattttaat
      721 tataatattt tattaaaaat ataaaggaaa aacctctttt aatgtagaaa cttatattga
      781 aggaggcgag aatcatgcga gaagaaaaaa gtaatgaaaa gtatgattgt tattggtgta
      841 atcaagagaa taatttctgt gtagaaataa aagataatat agtcatgata gatgatggca
      901 ctggtacgtt aaaacaagcc gttttcatag ggtataaaca aatccaaatt aatttaa
//
LOCUS       DAGSHT010000404          941 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.411, whole genome shotgun sequence.
ACCESSION   DAGSHT010000404 DAGSHT010000000
VERSION     DAGSHT010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.411"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            215..598
                     /locus_tag="MJB72_003765"
                     /pseudo
     CDS             215..598
                     /locus_tag="MJB72_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          426 a           71 c          137 g          307 t
ORIGIN      
        1 taaaataata caatagaatt agttattatt caacaggatt gtttgaaagt taaataaatt
       61 tatagatatg aacattattt tacacttaaa aacagactaa acacgtagag tagctggtaa
      121 aaattaaata tagtttttgt tctaaaaagc tcctattata atttaagcta gataaatttg
      181 aagaagtgac ataaattgaa agaaggaata tattatgcta aataaaaaat taccagatac
      241 agaattaaaa attatgaaat gcatatggaa tactggttat aaaactgtaa tatcaaaaga
      301 tgttgcagat gaaatagaaa aaacatatgg ttggaagcat acaactacaa ttatactttt
      361 agctagatta accaaaaaag gttttttaat atcatagaga attgggaggc atgtacacta
      421 tacagtatta ataaaagaga gagaatatct caaaatagag ggaaaaagag tttttagagg
      481 tttacataac aatcctttat cagaattaat ttcaaaatta catgatggag aagaaataac
      541 tgaagaaaaa ataatagaaa taggaagttg gataaaaagc tggaaatatg aagattaata
      601 aataaaaggt agctaataat ataagagctc ccttttttat gtggataata ctggggataa
      661 gttgtggaaa acttatataa aaattgtgga taaagtgttt tgctgacatt ttactgacaa
      721 aagttaaaaa aataaatgtt atatatatta taaaatgttg aaaaatagcc atatatataa
      781 aatataagta acactataga taaattactg tcttttatat tgactataaa gaacagtaat
      841 tgaataaata taaagatatt ttttgattat tattaattaa aaatttaatt tatatttaaa
      901 taatataaaa tttgaggtaa tattattttt taattgttca a
//
LOCUS       DAGSHT010000405          938 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.412, whole genome shotgun sequence.
ACCESSION   DAGSHT010000405 DAGSHT010000000
VERSION     DAGSHT010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.412"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            101..553
                     /locus_tag="MJB72_003766"
     CDS             101..553
                     /locus_tag="MJB72_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264801.1"
                     /translation="MKLSRKNITMQVLSKFIENNLEKIENVETKANQIVFRTIDGDKK
                     IKIKTSKNYSEGEDPNTYLWTSFSSEELEEDYDYYTIICNEDIEKAVVFTKEELKQHY
                     VLETKKKKNREIDFELYPHFIGRRCIDARVHLYKEPIDITKYVNNYIF"
     gene            587..907
                     /locus_tag="MJB72_003767"
     CDS             587..907
                     /locus_tag="MJB72_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264802.1"
                     /translation="MLYVGDYIDMKTLNIRYKDGFKIEEDTRLYPINSDINDKIGVLV
                     EHRKLNATNRYLRYGIQKISGKCYFFDVRNSVISKTKESAYNTFICPYCRERMFIVSA
                     FERQ"
BASE COUNT          438 a           77 c          147 g          276 t
ORIGIN      
        1 aaactaaaag aagctaaaca aagtttaata agtgaagcag tgacaggaaa gatagaaata
       61 ctagactaac tcttagtata tttgaacaga ggtgaaaaat atgaaattaa gtagaaaaaa
      121 tataacaatg caagttttat caaaatttat agaaaataac ttagaaaaaa tagaaaacgt
      181 agaaacaaaa gcaaatcaaa tagtttttag aactatagat ggtgacaaaa aaataaagat
      241 aaaaacaagt aaaaattata gcgaaggaga agatcctaat acatatttat ggacaagttt
      301 ttctagtgaa gaattagaag aagattatga ttattatact ataatatgca atgaagatat
      361 agaaaaagct gtggtattta caaaagaaga attaaaacaa cactatgtac tagaaactaa
      421 gaaaaagaaa aatagagaaa ttgattttga attatatcct cattttatag gaagaagatg
      481 tatagatgct agagttcatt tatataaaga accaatagat ataactaagt atgtaaataa
      541 ctatatattt tagtaatata tataatcaat aaaattatat tttacattgc tttatgtagg
      601 agattatatt gatatgaaaa cattaaatat aagatacaaa gatggattta aaatagaaga
      661 agatacgaga ctttatccta taaatagtga tataaatgat aaaattggag ttcttgtaga
      721 acatagaaaa ctaaatgcta ctaataggta tttaagatat ggtatacaaa aaatttcggg
      781 gaagtgttat ttttttgatg taagaaatag tgtaatatca aaaacaaagg agagtgcata
      841 taatactttt atatgtcctt attgcagaga aagaatgttt atagtatcag catttgaaag
      901 acaatgaagc atagtaccaa ttcacttgaa acattata
//
LOCUS       DAGSHT010000406          929 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.413, whole genome shotgun sequence.
ACCESSION   DAGSHT010000406 DAGSHT010000000
VERSION     DAGSHT010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.413"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            226..717
                     /locus_tag="MJB72_003768"
     CDS             226..717
                     /locus_tag="MJB72_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBY8264803.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          362 a           83 c          146 g          338 t
ORIGIN      
        1 taaaagtaat agtccttatt tatagtcaat attgcctata gaacttctga ttcatttaat
       61 ttttaggaaa aaataataat acttacgcta taaacacaga attttagttg ataatatagg
      121 tatttaaatt taagatgaaa taatactttt ctcaatatat tttattgtaa ctaagtttaa
      181 ttataagtta caattaaaaa aacgctactt aggaggaatg taattatggg gatgatagga
      241 tgttatacta aaataagtga agagaatgtt tttaaattac agcaagcaga agaaaactta
      301 caagattttg tttttgaaga tgccaacgaa aatagtacta tcaatattga taaagcatgg
      361 catgccattc actttacttt gacaggttgt ccttttggtg gagatgatga taatatattt
      421 agcaaattag tactaagtgg aaacatactt atggaaattg atggagaatt tcctgtcatg
      481 ttgattacag caaatgatgt aaaaaaacta tcaaaagcaa tgaacagttt ggaagaacaa
      541 gcatttagaa aaagatttaa tattaatgag atgttagaaa ataatattta tcctgttatg
      601 aatgatgaga gtgaagatga tttttttgaa tatgtatggg caaatctaat tgagcttaaa
      661 aagtttatac aagaagcatc tgatgatgga ctagctgtaa tattttttat aatgtgataa
      721 ttactaaaaa ttgtgaatat tagtgtcatc acatatctta gatatgaagc cttttgattg
      781 tatagaagaa aatacatatt atattagcct tttaaaatat ataagttaaa tttaaatctg
      841 taggcaaatt tgtcttatgt ctttataaat atttatatta gcatatataa attagtttta
      901 aatttttgta atatatagtt gcagatatt
//
LOCUS       DAGSHT010000407          883 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.416, whole genome shotgun sequence.
ACCESSION   DAGSHT010000407 DAGSHT010000000
VERSION     DAGSHT010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.416"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..876
                     /locus_tag="MJB72_003769"
     CDS             <1..876
                     /locus_tag="MJB72_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HBY8264804.1"
                     /translation="IVEQTDSVVFEWNIKENKSFVSQAWTEKFGYNNACKNIFKEIKD
                     KDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRTSITSIYNDENELLR
                     VVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETIVMNTGDKKDALFII
                     DLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGDEFVVYMQDYVTEVN
                     LHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFELFKNADRALYSIKAS
                     GKNSYCLFEEELYVQ"
BASE COUNT          348 a           77 c          164 g          294 t
ORIGIN      
        1 attgttgaac agactgattc tgtcgttttc gaatggaata tcaaagaaaa taaatcattt
       61 gtatcgcagg cttggactga aaaatttgga tataacaatg catgtaagaa tatttttaaa
      121 gaaattaaag ataaagactt agttcattct gaagataaag ctatatttga aggttttctt
      181 gaaagtatta aaaaaaagaa tatgcacaat caagcagtat atcgtttaaa aaaatctaat
      241 ggtgaatata tttggtgtag aacatcaatt acaagtattt ataatgatga aaatgaactt
      301 ttaagagttg tcggagtaat tgtagatgta gatagtgata taaaaaaata tgaagagtta
      361 agaactagag cagaaagtga ctcattgacc aatatataca ataaaggtac atttgaaaaa
      421 ttagtggaag aaactatagt tatgaataca ggtgataaaa aagatgcatt attcattatt
      481 gatttggatg attttaaaga aataaatgat aattttggtc atccatttgg tgattttgta
      541 ctaaaaacat ttgctgataa gatacaaact agttttggta gtaaagactt agtcggaaga
      601 attggtggag atgaatttgt tgtatatatg caagattatg ttacagaagt aaatttgcat
      661 aaaaaagcta aagaattaaa tagagtactt tcggataatt acactgattt gagttttagt
      721 tttgatgctt ctgtcagtat aggtattgca aggtatcctc aagatggaac ttcatttttt
      781 gaattgttta aaaatgcaga tagagcactt tatagcataa aagcatcagg taaaaattca
      841 tattgtttat ttgaagaaga actatatgta cagtgaattt gaa
//
LOCUS       DAGSHT010000408          882 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.417, whole genome shotgun sequence.
ACCESSION   DAGSHT010000408 DAGSHT010000000
VERSION     DAGSHT010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.417"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            102..>882
                     /locus_tag="MJB72_003770"
     CDS             102..>882
                     /locus_tag="MJB72_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY8264805.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVAN"
BASE COUNT          346 a          100 c          141 g          295 t
ORIGIN      
        1 tatattttca aacaagtaat ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata
       61 tatacataag caatataaat ctataaaaag gagtgttaca tatgatatct aataaaatgc
      121 aaactttagt tgcaaatagt tctgttataa gagctatgtt tgaagaagga aagaaacttt
      181 ctgatattta tggtgaggaa aatgtttttg attttagtat aggaaatcca agtgttgaac
      241 ctccagagac tattaaagca gttataaatg atattttaaa tgaagaatct ccaaatttag
      301 ttcatggata tatgaacaac tcaggatatg aggatgtaag agatgctatt gctgaacaca
      361 taaataaaaa agatggatta aatcttacaa gagaaaattt aattatgact tgtggtgctg
      421 ctggtggtct aaatattatt ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt
      481 ttgcaccgta ttttggtgag tataaaaatt atacagaaaa ctatgatgga aaattaatag
      541 aagttcctac aaatatagag acttttgagc cagatttaga tgctctaaaa aatgctatta
      601 ctccaaaaac aagagcttta ataataaaca ctccaaataa tcctactggt gtcatatatt
      661 ctgaagaatt attaaaaaat cttggagaat tattggattc aaaacaaaaa gaatttaata
      721 caagcatata cctaatttct gatgagccat atagagaaat aatttatgat ggtgctaaag
      781 ttccttgtgt actaaaatac tatagaaatt catttatagg atactcttat agtaaatcat
      841 tatctctacc tggtgaacgt ataggatata ttgttgctaa tg
//
LOCUS       DAGSHT010000409          876 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.418, whole genome shotgun sequence.
ACCESSION   DAGSHT010000409 DAGSHT010000000
VERSION     DAGSHT010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.418"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..838
                     /locus_tag="MJB72_003771"
     CDS             <1..838
                     /locus_tag="MJB72_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY8264806.1"
                     /translation="VVYLIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNIT
                     KDIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFL
                     KTVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNK
                     FIAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKG
                     MEEKDVTNSAIVTMIEFTYAEAPKSMRERYKINCEFLGLIPKDSDKIGVGVEYYDSLL
                     ND"
BASE COUNT          355 a           77 c          131 g          313 t
ORIGIN      
        1 agtagtgtat ttaatttaca ctatatttaa atatataaaa cttaaaaatt taataaatga
       61 cttatcttat gatgttgatg atagagagat tgtaaatctt tataaaaata tattagaaga
      121 atttaatata acaaaagata taaaactcaa atactcttat gaagttgaaa caccagcatt
      181 ttttaattca tgtgtgcttt tgccaccaca tgagtataaa ttgaaagagt tagactggat
      241 ttttagacat gagcttatgc attttaagag tagggacctt tatttaaaat atgtaatcct
      301 atttttaaaa acagtatatt ggtttaatcc atttatatat ataatggata aacatataga
      361 tttagattgt gaactttatt gtgatgaaag agttttaaaa aatcgaaatt cagaggaaaa
      421 aaaggattat gctttaacaa ttctaaatgc aatgagaaaa ggttcaaata cttcaaataa
      481 atttatagct ggactccata agcaatctga tattaaaaag agagttatga atatgtttaa
      541 tgaaaagtac aagaatggta tattaatggc attaacttta tgtttattat cttctattac
      601 ttttttgaag gttaattcaa cgagtattgt taatcttaat atattgccaa aaggaatgga
      661 agaaaaagat gttactaata gtgcaattgt tacaatgata gaatttacat atgcagaagc
      721 tcctaaaagt atgagagaaa gatataaaat aaattgtgaa ttcttaggtt taatacctaa
      781 agactctgat aaaataggtg ttggtgtaga atattatgac agtctattga atgattaaat
      841 aaatattatt ttaacagata ttaattataa agtttt
//
LOCUS       DAGSHT010000410          851 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.419, whole genome shotgun sequence.
ACCESSION   DAGSHT010000410 DAGSHT010000000
VERSION     DAGSHT010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.419"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..>851
                     /locus_tag="MJB72_003772"
     CDS             <1..>851
                     /locus_tag="MJB72_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HBY8264807.1"
                     /translation="RNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNK
                     EELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFF
                     DSYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKK
                     EADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEE
                     ICMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIF
                     LYVKDNV"
BASE COUNT          377 a           58 c          149 g          267 t
ORIGIN      
        1 atagaaatta taactcaaaa gatttaatga agatgataga acactcaatt ccagagaata
       61 tgttttataa agatgtgtat ggaaattata ttggatttaa ttcaggtttt ttgaatttag
      121 cttgtatgaa taaagaagaa ttattaggga agaatagtta taaaatatca gaagaagaag
      181 ctttaataga taagatattt gaaagtgata agggtgtagt tgaaaataaa aaagtagtta
      241 catttgagtt aaatataagt atgaatgatg aaaacaagtg tattgagatt acaaaaaggc
      301 cgttttttga tagctatgga agtgttattg gaataattgg aacagcaagg gatataagta
      361 gaagaaaaag attagaagag gaaatggata aaactaggat ggaatttttt gcaaatttat
      421 cccatgaact tagaacgcct ataaacttaa tatcatcttc attacaagtg atagaaaaaa
      481 aagaagcaga tttaatagaa tcaaatgata ctttaaaaag aaatttaggt ataataaaac
      541 aaaatggaaa tagaatatta agattagtaa ataatgttat agattttaca aaaatgcaat
      601 caggatattt agattttaaa ccagaagaat ctgatataat tgcatttata gaggagatat
      661 gtatgtctgt agctgatttt gcaagccaaa ataatataca acttacattt gatacagaaa
      721 tagaagaatt ttcaatgtta tttgattcag aaaaattgga aagaataata ttaaatttac
      781 tttctaatgg aattaagtac aataagaaag atggaaaaat aaatatattt ttatatgtga
      841 aagataatgt a
//
LOCUS       DAGSHT010000411          851 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.420, whole genome shotgun sequence.
ACCESSION   DAGSHT010000411 DAGSHT010000000
VERSION     DAGSHT010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.420"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(39..749)
                     /locus_tag="MJB72_003773"
     CDS             complement(39..749)
                     /locus_tag="MJB72_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264808.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          272 a          122 c          103 g          354 t
ORIGIN      
        1 gtttcaaaaa taaaattcta gagattgaac ttcaaaaatt aagattctaa catatacctt
       61 gtaaattttc ctacaacctc ttgatatttt ttacggctta taggaatttt ttcattctga
      121 actttggcat aagtttgagt tatactatct acatggttta gattgacaat atacctttga
      181 tgcgtacgta taaaattaga gggaagaaca gtttccatat catttaattt tccgtagtat
      241 tctaatgttt tatcaaaggt aactacagtt aattttctta gctcactttt aaagtagagg
      301 atatcttttg tattaatttt acaaagatta gttccaatct gaactgtaat aaagttttct
      361 ttccattgtt ctaatttttt tagtgcttct tttaatactt tacatatttt ttcagtttta
      421 taaggtttta agatgtagtt caatgctcct acatcatatc catgaaatac atagtcagaa
      481 aaacctgtga caaagatgat tatagtatcc tttgaatgtt ttcgtatagt ttttgctatg
      541 tcaacgccat tactttttcc aagttcaata tctaaaaaaa taatatctat gtaataattt
      601 tcgtgttcta gattgtatac tagactttct ccatttgaat attcttttat ttggtagggg
      661 tagccccaca aatccatttc tcttgtaatg attttattca gcatgtttct ttgttgtttt
      721 tcatcatcac aaatacaaat cgaagtcaat tttttatctc ctttcaaatt ttatatttat
      781 atagttaagt atataatata ttttgctacc tagatatata aaatgaaata acaattatat
      841 ccagatagca a
//
LOCUS       DAGSHT010000412          836 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.422, whole genome shotgun sequence.
ACCESSION   DAGSHT010000412 DAGSHT010000000
VERSION     DAGSHT010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.422"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..714
                     /locus_tag="MJB72_003774"
     CDS             <1..714
                     /locus_tag="MJB72_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264809.1"
                     /translation="LLSNLSLVSIIGRIIKDNSLKIEYEITKKRMNMQYKYYLNLQEY
                     QLKIRKLYHDMNNHIICIQNVYGKNEFADKYIKDINKQIKECNTIFNTQNMILDVILS
                     DKKSICDMNNISFLVDINFLECNFIEMPDICSIFSNIIDNAIEACIKIKDTSIQKKIK
                     LRGTVINRLFVIKCENTKINEVVLKNNKVITSKKDSFLHGIGISSVKSSVEKYNGNVE
                     IYSDKNKFTITIYIPLTRN"
BASE COUNT          372 a           60 c          132 g          272 t
ORIGIN      
        1 ttactatcaa atctttcatt agtaagcata ataggaagaa taataaaaga taatagttta
       61 aagatagaat atgaaattac aaaaaaaaga atgaatatgc aatataaata ttatttaaat
      121 ttacaggaat atcagttgaa aataagaaaa ctatatcatg atatgaataa tcatatcatt
      181 tgtattcaga atgtttatgg gaaaaatgag tttgcagata agtatattaa agatataaat
      241 aagcaaataa aagaatgcaa tactatattt aatactcaga atatgattct agatgttatt
      301 ttaagtgata aaaaatctat ttgtgatatg aataatatat cttttctagt agatattaat
      361 tttttagaat gtaattttat agagatgcca gatatatgta gtatattttc aaatataata
      421 gataatgcaa tagaagcttg tattaagatt aaggacacaa gtatacaaaa aaagattaaa
      481 ttaagaggaa ctgttataaa taggttattt gtaataaaat gtgaaaatac aaagataaat
      541 gaagttgtat taaaaaataa taaggttatt acaagtaaga aagattcctt tttacatgga
      601 ataggaataa gtagtgtaaa aagttcagta gaaaaatata atggaaatgt ggaaatatac
      661 tcagataaaa acaagtttac aattactatt tatataccat taactaggaa ctaattctag
      721 ttggggctct aaattgccca gttggagcag tagtattgaa agataaaata gaagatgcta
      781 tctttaaggc ataggatatt tggaggtgat aaaaatggtt gaatgctatg ctaata
//
LOCUS       DAGSHT010000413          821 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.423, whole genome shotgun sequence.
ACCESSION   DAGSHT010000413 DAGSHT010000000
VERSION     DAGSHT010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.423"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     misc_binding    93..356
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            507..>821
                     /locus_tag="MJB72_003775"
     CDS             507..>821
                     /locus_tag="MJB72_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBY8264810.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKK"
BASE COUNT          334 a           80 c          143 g          264 t
ORIGIN      
        1 tagattgcaa aatatatttt ttataaaaag gtattgacat tttttagaaa aaagaatatt
       61 attattttaa caacaaaatt caatacaata aattaaatcc tatgacaagg aagagtagtt
      121 aaatttaggt tcagtataga gagctgagga tggtggaatc ttagcaagaa aaaatttgat
      181 gaatggccct tagaggaata aattgaaatc atagttttga gagtagactt tatcgtcagt
      241 cgcacgttat agcgaataag gtatgtaagt acctgataat gagatgtatg aaaattgaat
      301 tttatattaa tttttatata atttaggtgg caacgcggat aatctccgtc ctattagttt
      361 aggacggagt tttttatttt actgaaaatt taaaagatag aaggtggtga gaaagatgtt
      421 atgaatttca aaagctaaag atgatgatat agtaggttgg caaaaaaata aattgaaaat
      481 taaaaaaata ggaggaagta caaaaaatga aaacaaatac aaaaaaatta actttaaatg
      541 caatactttt agcgatggga ttattaatac atcaaatcac accagctata ggattaccaa
      601 tgaagccaga tgtaccacta gcaatgttat ttgtaatatt ggttttaaat agagatgatt
      661 ataaaacttg tttgatagcg ggtatagtaa caggaatatt tacagcactt acatcaagtt
      721 ttccgggagg tcaaattcct aatgtaatag ataaaactct tacagcaaat atagtatttt
      781 tattaatgaa tattagttat aatatgcctt ttataaaaaa a
//
LOCUS       DAGSHT010000414          788 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.429, whole genome shotgun sequence.
ACCESSION   DAGSHT010000414 DAGSHT010000000
VERSION     DAGSHT010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.429"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            114..>788
                     /gene="rgbR"
                     /locus_tag="MJB72_003776"
     CDS             114..>788
                     /gene="rgbR"
                     /locus_tag="MJB72_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBY8264811.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVK"
BASE COUNT          374 a           55 c          123 g          236 t
ORIGIN      
        1 gataaaaata atttaaaaat gttatcctaa agtatcagat atacaaaatt atctttaaat
       61 atattataaa tattttaatt gtattaacta gaaattatgt aaggaggcat tttatgatta
      121 agatagcagt ttgtgaagat gaaaaagaaa cacaactttt aatagaagat taccttgaga
      181 atatattaaa agatataagt atagaatatg agatacaaaa gtatatatct ggagaagagt
      241 tactggaaag taatttaaaa gatatagata tattacttct tgatataaaa atggaaaaat
      301 taaatggaat ggatactgca agaaagatta gagaagtaga caatgaaatg gaaataatat
      361 ttgtaacttc attaatagat tatgtgcaag aaggttatga ggtaagagct tataggtatt
      421 tactaaagcc aatagagttg gaagaactaa aaaaacatgt gctaacttgt atcaaagata
      481 ttgaaataaa taaggaaagt catattacaa taaaaaataa gtctaataca tataagattt
      541 atttaaatga aataaaatat atagaagttc aaaaaaaaga tatgctaata cacacaataa
      601 acaagaattt tgatataaaa tatagtttag gcaaaataga aaaagaatta aatccatata
      661 aatttataag atgccataaa agttttatag taaatttaag atatgttgaa aatataaagc
      721 ctaatactgc aatactggaa agtggagaag aggtacctat tagtagatat agatataaag
      781 aagttaag
//
LOCUS       DAGSHT010000415          787 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.430, whole genome shotgun sequence.
ACCESSION   DAGSHT010000415 DAGSHT010000000
VERSION     DAGSHT010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.430"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>787)
                     /locus_tag="MJB72_003777"
     rRNA            complement(<1..>787)
                     /locus_tag="MJB72_003777"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAGSHT010000416          775 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.432, whole genome shotgun sequence.
ACCESSION   DAGSHT010000416 DAGSHT010000000
VERSION     DAGSHT010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.432"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(201..668)
                     /locus_tag="MJB72_003778"
     CDS             complement(201..668)
                     /locus_tag="MJB72_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBY8264812.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          298 a          146 c           46 g          285 t
ORIGIN      
        1 ataactatct tttatatatt ttttctaaat ttaaacttta gtataaatat ataagaaata
       61 tcataatatt tttaaaaact caaataaagc taaacaagta ttatattctt tattttagaa
      121 aaataaaaac tgtaagcaaa tttgttttca cacaagttta cttacagcct tcacctcaat
      181 tttactttaa tataatccat ctattctaaa actgattcaa tatattcgac agttacatcc
      241 attcttttag ctacctcatc tattgaaaga ccactatcta aaaaacgtct acacacagat
      301 gacatagttt ctccaacctc aataatatgc ccatctaaat cataaaatct aattgctctt
      361 tgaccccacc tatgctctat agctagatga acataatcta tatctttcat tgtactcaat
      421 ctttcaacaa aagtatcaaa attatcttcc tcaaaatata actcagcatc atttccattg
      481 aacttaactt cactgtcact cttattaata aattctaacc aaagcttttt ttcttgtaat
      541 gatatattac cttctaatac aacattttct ccaaaatcac agattacctt taaccccaaa
      601 acatcataat aaaacttttt tgatttctca atatcttcaa ctactactaa aacatttaaa
      661 aacttcatat tacctcctta atatacacat actttattta taatatataa ttagaattaa
      721 actataaagg tttcaataag gtctagtata aagaaaaaaa ttataagtct tattg
//
LOCUS       DAGSHT010000417          757 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.433, whole genome shotgun sequence.
ACCESSION   DAGSHT010000417 DAGSHT010000000
VERSION     DAGSHT010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.433"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(214..723)
                     /locus_tag="MJB72_003779"
     CDS             complement(214..723)
                     /locus_tag="MJB72_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBY8264813.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT          269 a          127 c           61 g          300 t
ORIGIN      
        1 attaaaaagt acaaaaacat ctacaataac aatttaaatc tttaagtgat atccatctca
       61 cacctaaagg agtgggctct ccgcactatt ctgtaattct tcaatacaat cattcttaat
      121 tttttatata atttaattat atattttatt ttaccaaaac cagcatacat aaataatact
      181 taaaatagat taaataaagg atactaaaac actttattga ggtatatgac ttgaaatata
      241 tgaactaact tcattaggag aaatatctaa aatagttgca aattcttcat ttaataacct
      301 ttcagcttct ttcattatat tatcatctgc ttgatgtgtt tttttaccaa tagattttac
      361 atactctctg ttattatcta tacttcttac taattttatt aactcttcac atttaccacc
      421 tctgagtatt tttttaaatt gctcacttct taatttttca ttatctatcc atgatgtatc
      481 catattagga atatcattta ttaatgaaag cacattaatt ttagaaagca actttctcat
      541 tggtactttt tcattatcta caggaatttt tattactgta ttattagaat aaataggact
      601 caatacataa tactcttttt ttattccatt tgtaaatcgt tcattcgtta tatccatgac
      661 tttgcagact cctgtcatac catacattat ataatcatca atcttaaaca aaaacatcac
      721 catccttaat aaaaaaaata gttttgacaa ctaaatc
//
LOCUS       DAGSHT010000418          753 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.435, whole genome shotgun sequence.
ACCESSION   DAGSHT010000418 DAGSHT010000000
VERSION     DAGSHT010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.435"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            400..621
                     /locus_tag="MJB72_003780"
     CDS             400..621
                     /locus_tag="MJB72_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264814.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          343 a           62 c          102 g          246 t
ORIGIN      
        1 tacttattat aaagaatata taactttcaa taatagttca ttttaaaatc acaaaaaatt
       61 cataatgaaa ctgtattata taaatataaa taagcttaaa accataaaat acaccaacta
      121 taatgatgag atttccttac atcctccata taagtcatca ttatagtaac ccaacaatta
      181 agttactatg aatttaaaaa ataacaccga gcatataatc tatgcttagt gttattttta
      241 ttttgaaata aaaaatattt aatttattga aatgtataga tttagatagt ttaaactata
      301 tttaagactt aaaaattata attgatatta caaataaata tatgtacttt gggaaaaata
      361 aatataggaa gtattatatt tacagaggag gtagttttga tggatagatt aaaaagagat
      421 atgaaaaata tgaaagaaga tttaaaagaa ggtgttaaaa agacagctta tgttatgaaa
      481 aatacagctg aagatttgaa agatgatgca caaggtgcta tggtaaactt agaaatgaaa
      541 aaagatgaga tgatggaaag atatgaacaa aagaaatttt ctaaagaatt agagaagcaa
      601 atgaagaatg aaatgaaata agcttttatt tatgaataac aaattattgt attcatataa
      661 aaagtagcca gtaatattaa atagtttatt taaggaaata gactttctaa aaataactgt
      721 attagattta gaaagtctat ttcaaattag ttc
//
LOCUS       DAGSHT010000419          753 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.436, whole genome shotgun sequence.
ACCESSION   DAGSHT010000419 DAGSHT010000000
VERSION     DAGSHT010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.436"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..>753
                     /locus_tag="MJB72_003781"
     rRNA            <1..>753
                     /locus_tag="MJB72_003781"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAGSHT010000420          744 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.437, whole genome shotgun sequence.
ACCESSION   DAGSHT010000420 DAGSHT010000000
VERSION     DAGSHT010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.437"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..281
                     /locus_tag="MJB72_003782"
     CDS             <1..281
                     /locus_tag="MJB72_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264815.1"
                     /translation="DKIADYITFIHKGKIVFSENKDELIDTMGVLKCKPSDFDNLSRE
                     DYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIEEIMLFYVRGDK"
     gene            281..>744
                     /locus_tag="MJB72_003783"
     CDS             281..>744
                     /locus_tag="MJB72_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY8264816.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCF"
BASE COUNT          260 a           72 c          112 g          300 t
ORIGIN      
        1 tagataagat agcagactat attacattta tacataaagg aaaaattgta tttagtgaaa
       61 ataaggatga attaattgat actatgggag ttttaaaatg taaacctagt gattttgata
      121 acctttcaag agaggattat tcatattatc gtaaaagcca atttggatat gaagtacttc
      181 ttaaagataa gcacagattt atctcaagac atccaaactg tattgttgat aatacgtcta
      241 tagaagaaat tatgttattt tatgtgagag gtgataaata atgaaaggtc taattttaaa
      301 agatttactt aatttgaaag gaaatataaa gtttatactt ttattcatca ttatgtttgg
      361 ctttatgtct tctttaggag atggaaatgt taataacttt ataggtgtta ttatagtttt
      421 atgtactaca atgattgttt caacattttc atatgatgat ttgaataaat gggatagtta
      481 tgtacttaca atgcctatta atcgcaatga tattgtatta agtaaatatt taactatgtt
      541 aatatttagc tttataggtg ttcttgtttc acttattgtt agtgtaacta ttggttattt
      601 taaaaataca cttatattaa atgaaacact acttattaat gcactaatct tatcaatttc
      661 agtatgtttt ggtagcctta ttctaccact tatttataaa tttggtacag aaagagctcg
      721 tttattaatg attttatgct tttt
//
LOCUS       DAGSHT010000421          722 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.439, whole genome shotgun sequence.
ACCESSION   DAGSHT010000421 DAGSHT010000000
VERSION     DAGSHT010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.439"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            463..>722
                     /locus_tag="MJB72_003784"
     CDS             463..>722
                     /locus_tag="MJB72_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY8264817.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTD"
BASE COUNT          289 a           86 c           71 g          276 t
ORIGIN      
        1 caatgttgat attatctctc ctagttgata ttatctttcc taaaatttct tttaattaaa
       61 aaacaacatt agttgatata taaagtatat tttaagcaac tgaaaaaaac aataataatt
      121 gatatatcaa ctaattaatg tagattaatt catttttata atttatatac tttttaatta
      181 atctacctta tttaattaaa atccttcctt tctatttatc cattttcaac ttaaattata
      241 agatttcatc agctatattt ttgcatacca ataattatta aatatcaaat atatattcta
      301 ttaaaatttc taataatttc atccttgatt tatttaatat ataaaaatat actaacttta
      361 atgattaagt tttgaaatat aactaagaat ttgataattt attgagattt ttatactaaa
      421 atatacatac tatacaaact taatttagaa gggagcattc ttatgaacaa taaaatactc
      481 gtcgtagatg atgaggaagg tataataaca ctcttaaaag attactttga aatgaatggt
      541 tatgaggttt acactgctca aagtggaaaa gacgcattaa aaaaaatatc caaaaatcca
      601 gatatcattc tacttgatat aaacatgcct gaaatagatg gaattgaagt ttgtaataga
      661 attagagatt atgtatcatg ccctattcta tttcttacag ctagaattga aaatactgac
      721 aa
//
LOCUS       DAGSHT010000422          713 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.441, whole genome shotgun sequence.
ACCESSION   DAGSHT010000422 DAGSHT010000000
VERSION     DAGSHT010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.441"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..>713
                     /locus_tag="MJB72_003785"
     CDS             <1..>713
                     /locus_tag="MJB72_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8264818.1"
                     /translation="FNKIIIFINGNVTYFVDGIPYKLKPWDILFISNNEIHKPVIDSN
                     VFYERIAIWISPEFMKKNSDATSDLEKCFKIATENKCNRLRLNIHDIESIKSFIEQIN
                     ISESNTEFGNEILRNALFIQLMIFLNRIFLKNENMESTEDVFYDETIKKVLNYINSNI
                     DDKLSIDSISSEFFISKYYLMRKFKSHTGTSIHNYIVQKRLILAKSLITNGHLMSEVC
                     NKCGFNDYSSFVRAFKKNY"
BASE COUNT          290 a           76 c          102 g          245 t
ORIGIN      
        1 ttttaataag ataattatat ttataaatgg aaatgtaact tattttgttg atggtattcc
       61 ctataaacta aagccttggg atatactctt tatcagcaat aatgaaatac ataaaccagt
      121 tatagattca aatgtctttt atgagagaat tgcaatatgg attagcccag aatttatgaa
      181 aaaaaatagt gatgctacta gtgacttaga aaaatgtttt aaaatagcta cagagaataa
      241 atgcaataga ttgcgtttaa atatacatga tattgaatct attaagagtt ttattgagca
      301 aataaatatt tccgaatcca atactgaatt tggaaatgaa atattgagaa atgcattatt
      361 tatacaactt atgatattct taaacagaat tttcctaaaa aatgaaaata tggaatcaac
      421 agaagacgtt ttttatgatg aaactattaa gaaagtatta aactatataa attcaaatat
      481 agatgataag ctttcaatag atagcatatc gtctgaattt tttataagta agtattatct
      541 tatgagaaaa tttaaatctc atactggaac gtctatacac aattatatag ttcaaaaaag
      601 attaattttg gcaaagtcac taataaccaa tggtcacctt atgagtgaag tttgtaataa
      661 atgtggattc aacgattatt ctagttttgt aagggcattt aaaaaaaact acg
//
LOCUS       DAGSHT010000423          710 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.442, whole genome shotgun sequence.
ACCESSION   DAGSHT010000423 DAGSHT010000000
VERSION     DAGSHT010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.442"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..293
                     /locus_tag="MJB72_003786"
     CDS             <1..293
                     /locus_tag="MJB72_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBY8264819.1"
                     /translation="KISNNYIKKGNSIGKSLNLANVFPKLFISMANTGEESGKLDKSL
                     NVINKFYENELNNKIEQIMKFIEPMLIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            347..706
                     /locus_tag="MJB72_003787"
     CDS             347..706
                     /locus_tag="MJB72_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBY8264820.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
BASE COUNT          296 a           70 c          118 g          226 t
ORIGIN      
        1 taaagatttc caataattat ataaaaaaag gaaatagcat aggtaaatct ttgaatttag
       61 caaatgtttt tccaaaatta tttatttcaa tggcaaatac aggagaagaa agtgggaaac
      121 tagataaaag tctaaatgta ataaataaat tttatgaaaa tgaattaaat aacaaaatag
      181 agcaaataat gaaatttata gaacctatgc taattgtatt tatgggatta attattggta
      241 cttttatttt agcaatggtt gttcctatgt ttgatgcaat tactaatttt tagagagaaa
      301 aataaatttt attagatgaa acttttgaaa aggagagaat atttaattga taaatttgat
      361 aaataaaaaa cgaaaaggat ttacacttgt tgaaatgatt gtagtagtaa ctattttagg
      421 cgttatatct agtatagcat tagttaagta tagtaaggtt caagaaagtg ccaaattaaa
      481 tgcagactat acgaatgctg ctaatatagt aaccgcagct agcatggcaa ttaatgatga
      541 tgaaaagaca atagactctc taagtgtaga gacattgaag gaaaagggat acctaaatac
      601 tgttccagtt cctcagagta catcaggtaa attcgaactt gtcataaatg atagcggaac
      661 agatataagc gtaaatataa attctaagca attttatcca aaataatatt
//
LOCUS       DAGSHT010000424          707 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.443, whole genome shotgun sequence.
ACCESSION   DAGSHT010000424 DAGSHT010000000
VERSION     DAGSHT010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.443"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..539)
                     /locus_tag="MJB72_003788"
     CDS             complement(<1..539)
                     /locus_tag="MJB72_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBY8264821.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKT"
BASE COUNT          238 a          121 c           50 g          298 t
ORIGIN      
        1 gtttttatat ctaactcatc tttactaaat actttttttc ctttatcatc ataataaaaa
       61 tcacttatat ataattcttc acccattata ctcttactat ttagacttct atctattttt
      121 aaagctttgc aaagttttcc tggtccgtta gtaatatttt ttacttgata tttactcaac
      181 tcagtgtaag ctttgttgta tctattcata gatatctcat ctaaagaagt tataggctct
      241 acccctctta ttaatacaca ctctggcaca ttttctatat ttgcagataa attcaagcaa
      301 ttatacatac cataaattaa ataaacatat atatgtccac catctaaata taatggctta
      361 gttctatctg tctttttatt gccatataca tgtgctcctt tatcatttac acccatgtat
      421 gcttctgtct ctatgatttt ggtaacaatt actttatttt catatttcct tattagatat
      481 ttacctaata ttgatttggc taaatcaatt ccattttttc taaaaaaatc cttttccaaa
      541 atttcacctc ataaaattta tttaaaacgc atcaaataca actaaagtaa taaataaaat
      601 taactcttat taatgtgata tatataaaca ataaatttag acatttatta tttatatata
      661 tcaaaaaatt ctatctcaaa cttttcaata ttatcattgt aatttga
//
LOCUS       DAGSHT010000425          700 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.445, whole genome shotgun sequence.
ACCESSION   DAGSHT010000425 DAGSHT010000000
VERSION     DAGSHT010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.445"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            1..700
                     /locus_tag="MJB72_003789"
                     /pseudo
     CDS             1..700
                     /locus_tag="MJB72_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
BASE COUNT          316 a           51 c           94 g          239 t
ORIGIN      
        1 ttatctgttg ctttaataac aaatttattt actatattaa taatttatat aaatagaaaa
       61 atgtatcaat ttggtgggta ttaggaaata tatatagttg ggtatattgt gatacctagg
      121 ttaatactat ctagtagctt agtcattatt atagtgttta taaaaataat taaggaaaat
      181 aagttaaaat tgcaaaatga agtaataaaa aataagttag atatgcagta ttctcattat
      241 ctaagtattc aagaatcaca cgctaaagtt aagaagcttt atcatgatat aaataatcat
      301 atatattgta tagaaaatct caaatataaa aaaggaaaat tgatgggtat gtaaatagtt
      361 taaaagatta aattaaagaa tttaaatcaa tttacaatac aggtaatgtt atattggata
      421 taataataaa tgaaaaaagt gatgtaagtg aaaaaagaaa aattaaattc atttgtgata
      481 tcaatttttc caaagtgaat tttataaaac caatagatgt atctaatata tttgcaaata
      541 cttagataat gcaatagaag cttgcgataa gatacatgat aaaagtattg ataaatatat
      601 acaaataaaa ggaactatct caaagtccta tttcgtaatt aaatgtgaga atagtaaaat
      661 aaattcaatt agaaagaata aaagtttatt attaacagat
//
LOCUS       DAGSHT010000426          694 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.446, whole genome shotgun sequence.
ACCESSION   DAGSHT010000426 DAGSHT010000000
VERSION     DAGSHT010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.446"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..632)
                     /locus_tag="MJB72_003790"
     CDS             complement(<1..632)
                     /locus_tag="MJB72_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY8264822.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIF"
BASE COUNT          276 a          109 c           80 g          229 t
ORIGIN      
        1 aagaaaatag tagaaaagac ttgagacaaa ccagttgcaa taccagcacc ttttacaccc
       61 catccaaatt caattacaaa caagtaatca agtatgatgt ttgtaagagc agatattatt
      121 acaccaacag tagctaatat tggaaagcca tcagttttta ttataacttc taaagaataa
      181 gacactataa agaatccgtt aaaaaatatt attattccta aataatcttt aaccatacca
      241 attgtagatt ctgtagctcc taaaaatagt gctaatctat ctaaattaaa aaaagttata
      301 gctaatataa ttattgataa tataattatt gatactaagt taaatgaaaa gacttcgtta
      361 gcagatttaa tatctttttt ccccagatag attgcaatta tggtagatgc accagttgaa
      421 aataatagag aaactgcaaa tataaaatta ataaaaggca ttgatatatt gacagcagct
      481 aaagctaatt ctccaactcc tttacttaca aatattccat caaccattgt atatagtgag
      541 aaaacccaca ttgcaactac agatggtgct aggtatttaa aaaatctttt acctagactc
      601 atgttttcgt ttatagatat ttttgtatcc atttactatc actccctgta aatctattat
      661 aaaattacga aactattaat taataatttt attt
//
LOCUS       DAGSHT010000427          675 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.448, whole genome shotgun sequence.
ACCESSION   DAGSHT010000427 DAGSHT010000000
VERSION     DAGSHT010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.448"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     repeat_region   40..662
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=40..68
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
BASE COUNT          244 a           69 c           98 g          264 t
ORIGIN      
        1 aatttcccta atgctccatt ttcattataa tctggtgtag ttttatatta actatatgga
       61 atgtaaatgt aggtgtcgga gagctaggaa actatagcat aatggtttta tattaactat
      121 atggaatgta aatcgttctt ggaatgctaa aactcgtcct tcttggaata agttttatat
      181 taactatatg gaatgtaaat ctcccaggag taccgctata aaaataaatc atacaatgtt
      241 ttatattaac tatatggaat gtaaataatt tttgaaatgc actgaatact catttattac
      301 atcgttttat attaactata tggaatgtaa atgcataatt tagataacct tttatatcat
      361 caatataagg ttttatatta actatatgga atgtaaatag tatcgacatg gcaaatgggc
      421 taaaaaaaag tgattggttt tatattaact atatggaatg taaataggaa caaattgcta
      481 aaaaattagg ggttggttat tcgttttata ttaactatat ggaatgtaaa tctgagaaag
      541 ttttattctt ttttaatgtt acaaatcgtt ttatattaac tatatggaat gtaaataaat
      601 tattccctaa aaaaagttta tttctttcta ctagttttat attaactata tggaatgtaa
      661 atattacagt ttctt
//
LOCUS       DAGSHT010000428          664 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.450, whole genome shotgun sequence.
ACCESSION   DAGSHT010000428 DAGSHT010000000
VERSION     DAGSHT010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.450"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>664)
                     /locus_tag="MJB72_003791"
     rRNA            complement(<1..>664)
                     /locus_tag="MJB72_003791"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAGSHT010000429          662 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.451, whole genome shotgun sequence.
ACCESSION   DAGSHT010000429 DAGSHT010000000
VERSION     DAGSHT010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.451"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            11..388
                     /locus_tag="MJB72_003792"
     CDS             11..388
                     /locus_tag="MJB72_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY8264823.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          266 a           54 c           98 g          244 t
ORIGIN      
        1 gtaaaatcat atgactatta ctaagatacc accagcagaa ttaaaagtca tgaaatttat
       61 ttggagttca gattttacag tagcatcaaa agatgtcatt gaagctatgg aaaaactata
      121 taattggaag caaactacaa cactaacact tttatcaaga ttagtaaata agcaattctt
      181 aagtgcaaga aaaattgata gacatacaca ttatacagtt cttattggag aagaagagta
      241 tttacatttt gaaacaaaaa cttttttaga taatatgcat ggaagttcta ttgagagttt
      301 tatgaaatcc ttgttcaaaa agggggtaaa tgaagaagaa ttgaactctg ttgaaaaatg
      361 ggttaaggat attaaaaaag atgaataatt tggtgtagtt aattatcata aaataaatag
      421 tttttacaat aattagttaa atgaatcaaa tataaagttt tatgtaaaat ttgtgatttt
      481 tataaatatt tttgtcttgt tatttaatat taatacaata agtttgatgt aaaatcaaac
      541 ctgtttgaat ttaatttata ttgggtttgt cattaatatt attttagttt gatattgtat
      601 acgaatttaa aagtcagaat ttttgtatat taatcatgta aattaattgt tttagatata
      661 aa
//
LOCUS       DAGSHT010000430          592 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.457, whole genome shotgun sequence.
ACCESSION   DAGSHT010000430 DAGSHT010000000
VERSION     DAGSHT010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.457"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            34..>592
                     /locus_tag="MJB72_003793"
     CDS             34..>592
                     /locus_tag="MJB72_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY8264824.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVT"
BASE COUNT          223 a           60 c          112 g          197 t
ORIGIN      
        1 atatacgagc taaaagttat agggggtggc ataatgaatg acaacatagt agaagttaaa
       61 aatataaata tgaacttcta cactaaggaa ggcgagcttg aagtattaaa ggatgtcaat
      121 tttaacttga aagaaggaga aatattaact ttgcttggtc cttctggaag tggtaaatct
      181 acaatattaa atattcttac taatctttta aaaccgacta gtggtgatat aaagattact
      241 ggaaatattg gatatatgtt tcaaaaagat aatttgcttg aatggagaaa tataatggat
      301 aatataacta ttggattgga aattcaaggc aaaaaagata aaaaatcttt agatagagtt
      361 gaaagccttt taaagactta tggattatgg gattttagaa gtatgtatcc aaaagaatta
      421 tctggaggta tgagacaaag agttgcgctt attagaacac tttctgtaaa tccagatatt
      481 ctacttttag atgaaccttt ttcagctctt gattatcaga ctagattatt agtttgtgat
      541 gatgtttata gtattattaa gaatgagaat aagtctacta ttttggttac ac
//
LOCUS       DAGSHT010000431          577 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.458, whole genome shotgun sequence.
ACCESSION   DAGSHT010000431 DAGSHT010000000
VERSION     DAGSHT010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.458"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..>577
                     /locus_tag="MJB72_003794"
     rRNA            <1..>577
                     /locus_tag="MJB72_003794"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAGSHT010000432          531 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.465, whole genome shotgun sequence.
ACCESSION   DAGSHT010000432 DAGSHT010000000
VERSION     DAGSHT010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.465"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            142..>531
                     /locus_tag="MJB72_003795"
     CDS             142..>531
                     /locus_tag="MJB72_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBY8264825.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNK"
BASE COUNT          239 a           39 c           88 g          165 t
ORIGIN      
        1 aatgtcaaaa ttataaaaaa attctactta tataaaatat aaagaggtta tatgtacata
       61 tgtgtagaaa taaataataa aaatatcaat taatgttatt agggggggtt actcattagt
      121 aaattagcta atacacttag aatgatgata ttaattcaaa gtagaggcaa aatgaaatgc
      181 aaagaattat cagaagagtt ggaagtaagt gaaaggcaaa taaaaagtta taagatgtat
      241 ttagagcaag ctggtatatt tataaattca acacctggga tttatggtgg ttatgaaata
      301 gataaatgta atagcatctc attaattaaa ttattagata gtgaagtttc aattctagat
      361 atgataaatt cacagttaga gtataataat gatatttaca aaaatgaatt taataatatt
      421 gtagaaaaaa taaaagcagt attaaataca ggagaaaaaa gcgatacata tatggattat
      481 tttacagttc aagcacaacg taattgtgat tatgagtctg aaaaaaataa g
//
LOCUS       DAGSHT010000433          522 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.467, whole genome shotgun sequence.
ACCESSION   DAGSHT010000433 DAGSHT010000000
VERSION     DAGSHT010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.467"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..>522
                     /locus_tag="MJB72_003796"
     CDS             <1..>522
                     /locus_tag="MJB72_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264826.1"
                     /translation="RDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEKIRSIK
                     NSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVVFSLII
                     GYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKSIEAIE
                     KFDKISKDLNDFN"
BASE COUNT          210 a           54 c           76 g          182 t
ORIGIN      
        1 aagggatgac tactatacat atagtaaaga aaatccttat gcagacgtaa atataacttc
       61 atttatagag gaagttgtat ctgatttaaa gcataataat ttgccattgt tagaaaaaat
      121 tagaagtata aaaaattcat cttcaataag tatattactt ggggttttag gtacgtttgt
      181 tggtctttct acaatgcttt tatgtgttga tactaaagac attataaatt cacttccttc
      241 aactattagt agtatgcaaa ctgcatttac aactagtata tttggagttg tattttctct
      301 aattataggt tactttacta agataaaaga ttgtgaacat gttttgatac aaataatgtt
      361 aaaatctgaa aatttactaa catctgaaat aactcatttt aagtcagaaa gaatggattt
      421 aaaagtagaa gaggtaaaaa atacaataaa acaaataagt aagtctatag aagctattga
      481 aaaatttgat aaaatatcta aagatttaaa tgactttaat ga
//
LOCUS       DAGSHT010000434          512 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.denovo.469, whole genome shotgun sequence.
ACCESSION   DAGSHT010000434 DAGSHT010000000
VERSION     DAGSHT010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.denovo.469"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            136..>512
                     /locus_tag="MJB72_003797"
     CDS             136..>512
                     /locus_tag="MJB72_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264827.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHAS"
BASE COUNT          183 a           62 c           80 g          187 t
ORIGIN      
        1 cttttatttg gatttaatta aattttaatt gtcaaaatct actatgtatt cttattgcca
       61 ctacttctat gttgtgctat aattactttg ttttaagcga atattttata ttaattttat
      121 tatttggagg ttcttatgct aggtgaaaag atgagaaata taagaaaaag caagaaaaaa
      181 acattaagtg atgtttccaa attgacagat ttatctataa gttatatctc tcaaatagaa
      241 agagatgcta ttgaaccatc cttgtcttca ttgagaaaaa tcgctgaagt tttagacact
      301 cctttatata tgtttatgga tgacaataat actgatgatt tagtcattag aaaagaagat
      361 agagttatga tgaaatttcc aaagagtgaa atgttttacg agatagtatc tccaatgcct
      421 acaactgagt ttacaccttc aattttattt gtagagtttg aacttaaacc agaaagtgag
      481 gatgccaaag attatatata tcatgcatca ga
//
LOCUS       DAGSHT010000435        76178 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.guided.002, whole genome shotgun sequence.
ACCESSION   DAGSHT010000435 DAGSHT010000000
VERSION     DAGSHT010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 76178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.guided.002"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            320..1519
                     /gene="hadA"
                     /locus_tag="MJB72_003798"
     CDS             320..1519
                     /gene="hadA"
                     /locus_tag="MJB72_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBY8264828.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1558..2358
                     /gene="hadI"
                     /locus_tag="MJB72_003799"
     CDS             1558..2358
                     /gene="hadI"
                     /locus_tag="MJB72_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBY8264829.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2373..3599
                     /gene="hadB"
                     /locus_tag="MJB72_003800"
     CDS             2373..3599
                     /gene="hadB"
                     /locus_tag="MJB72_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBY8264830.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3599..4726
                     /gene="hadC"
                     /locus_tag="MJB72_003801"
     CDS             3599..4726
                     /gene="hadC"
                     /locus_tag="MJB72_003801"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBY8264831.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4774..5907
                     /locus_tag="MJB72_003802"
     CDS             4774..5907
                     /locus_tag="MJB72_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBY8264832.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            5940..6725
                     /locus_tag="MJB72_003803"
     CDS             5940..6725
                     /locus_tag="MJB72_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY8264833.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6744..7781
                     /locus_tag="MJB72_003804"
     CDS             6744..7781
                     /locus_tag="MJB72_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY8264834.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8264..9895
                     /locus_tag="MJB72_003805"
     CDS             8264..9895
                     /locus_tag="MJB72_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY8264835.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10030..10956)
                     /gene="fba"
                     /locus_tag="MJB72_003806"
     CDS             complement(10030..10956)
                     /gene="fba"
                     /locus_tag="MJB72_003806"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBY8264836.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11286..11615
                     /locus_tag="MJB72_003807"
     CDS             11286..11615
                     /locus_tag="MJB72_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBY8264837.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            11615..12337
                     /locus_tag="MJB72_003808"
     CDS             11615..12337
                     /locus_tag="MJB72_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBY8264838.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12364..13731
                     /gene="rlmD"
                     /locus_tag="MJB72_003809"
     CDS             12364..13731
                     /gene="rlmD"
                     /locus_tag="MJB72_003809"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBY8264839.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHVECVVLM
                     SRVEK"
     gene            complement(14046..14525)
                     /locus_tag="MJB72_003810"
     CDS             complement(14046..14525)
                     /locus_tag="MJB72_003810"
                     /inference="COORDINATES: protein motif:HMM:NF018184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY8264840.1"
                     /translation="MKYKLENKGTFKVVSIRETITPNEKGIFVPNINQTTRENFCQYF
                     KQLSERRVGEILYIAVKQWEELNDYYVGVEISEKFSNPLPESLVELEIPMQTWAVFEL
                     PSLSYSVINDTWYKLFTEWFPDHGYELAENVQFFREKNDIHRYELWVPIIKGNNKRL"
     gene            14917..15325
                     /locus_tag="MJB72_003811"
                     /pseudo
     CDS             14917..15325
                     /locus_tag="MJB72_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215340.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15322..16230
                     /locus_tag="MJB72_003812"
     CDS             15322..16230
                     /locus_tag="MJB72_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBY8264841.1"
                     /translation="MMFIATKTDDGEIKGKISFYCRVLHVSRQAFNKYLKAKDAPWKY
                     QELADAMLDICSEDECNDTYGRIRMYQALQLKQPEGIHIPGERTVYRVMDEIGLNHKP
                     KRKPNGITKADKEARKSDDLIKRDFTAEKPLKKCITDMTEIKASDGKLYVSAIFDCYD
                     LAVLGLAMDTNMKAPLCEQTLDNAYRAYPMLRGAILHSDRGTQYTSGLYRKAINKYGI
                     LQSMNSAGGRCHDNARCESMWARFKEELLYGRYDTSSMTVEQLKTLIWRYFIGYWNNR
                     RICSANGGLPPMVKRQQYYDSLQEAA"
     gene            16491..17369
                     /locus_tag="MJB72_003813"
     CDS             16491..17369
                     /locus_tag="MJB72_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8264842.1"
                     /translation="MKPLKQLNLAMTYIEDNLSKEIDFQMVAQLACCSEYHFRRLFSF
                     LSGMTLSEYIRQRRLSQAALELCNADVKIIDVAMKYGYSSPDAFTRAFQGFHGVTPRE
                     AKTIGTALKAIPPMTFQLTIKGGSKMDYRIVEKEAFYIVGIKRRITLIYRGVNPDVKA
                     MYLSLSKEDFRKLAQLSNMELEGIVNASVNFEVDRFEGSSLDHFIGVATTIKPEGKWD
                     VLSVPASTWAVFTVEGTFPEALQTTWAKIASEWLVPQVSGYELNEGPELLCYNKDEYL
                     DSSNFRCEIWIPVVKK"
     gene            17448..18176
                     /locus_tag="MJB72_003814"
     CDS             17448..18176
                     /locus_tag="MJB72_003814"
                     /inference="COORDINATES: protein motif:HMM:NF023031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264843.1"
                     /translation="MKYKFETIKKDYSDFSSGRVLYNAANTTAFPVRLASEVIQRAFH
                     ILDKDGASGPYKIYDPCCGGGFLLTTIGFLYHNLISELIATDYDVEVLEIAKKNLSLL
                     SEEGLDRRMKEIKGYIEVFRKDSHIQALKSVNQLKTLIGEANINIHCAQRDITDMSAF
                     PIKEVNIIITDIPYGINVEWNSGSNNPIQNLFENSYKALDKNHSLMVVIADKKSKLEH
                     KLFKRVEYFKLGKRQVAFFKPNRC"
     gene            18301..19128
                     /locus_tag="MJB72_003815"
     CDS             18301..19128
                     /locus_tag="MJB72_003815"
                     /inference="COORDINATES: protein motif:HMM:NF012797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264844.1"
                     /translation="MIKNRVVKISKEDNDFQHFEVIKRNRNKRHKHKEFFVEGVRNIN
                     EAIRNKWEISSFIYSGENELSGWAKSILSNVKVSFQYELPNHLMDKLSRKMDTSELLA
                     IVKMKTNDLSRIKLTKKNPLIVVMDRPSNKGNLGTLIRSCDALGANGLIITGHAVDLY
                     DPETIASSVGTFFSFPVLNLPSHKELYEWIQSLKSQHPNFQIIGTSAHGNKIINQCDF
                     LKPTVLLLGNETLGLSRNYKEISDLLVEIPMDGVASSMNVSCAASIVLYEIKRQRSL"
     gene            complement(19350..21857)
                     /locus_tag="MJB72_003816"
     CDS             complement(19350..21857)
                     /locus_tag="MJB72_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBY8264845.1"
                     /translation="MRDILFPFQETALSELHDKINKAHLMWSERDPQIISFSAPTGSG
                     KTIIMTTLFEDILYGNADNIGEPDSVFIWLSDSPELNEQTRLKIESKSDKIRVRDLVT
                     IDSTFNAEYFEGGCIYFLNTQKLGSDKLLTGTSDNRQYSIWETLTNTAKRIPKKFYVV
                     IDEAHRGTYTSMQAENKAQSIMQKFIKGSEDDGLCVMPLVIGVTATPQRFDSLIAGTT
                     STVQKVIVPPEHVRESGLLKDRIIIHYPDIQLNADMTMFKGAVDNWRKKCIHWKTYCE
                     REDEKMVNPILVIQVEDGNDRIATQTDLGACIDLLEETLGRKLQPGEAVHTFNDRGTL
                     KVRDVEIQQIEASRIQDEENVLVVFFKMNLSTGWDCPRAETMMSFRSAQDYTYIAQLL
                     GRMIRTPLARRISSDAELNSVSLFLPYFDEETVKNVVNALRDSEAAMPTEAGTSKELV
                     TLGRNLAYSDVFEAMDNLITYRIDSSRKQAPLKLLIQLSRALTMDGIDLGAQKAVKNA
                     VLSKMDDEILRIKESGDYDTRGASITGFALGTLIFDYGDNAYSFDEETQTMTMSEFDI
                     SRHFEQAGKLLGEGLHKEYWIRHSTRDHIDVKKEIIILTNDTDAMERINDYAEKEFIS
                     LYENNKRSIARLSEARKNVYERLTNASVQPISVPWILPNSIDFSVPENSMKFEQHLYC
                     SEDGTFETSLNTWEIGVVTEELKNGAVCWLRNLDRKKWSLEIPYEVGGVTTSMFPDLV
                     IVRSDAQGYVFDILEPHDPSRKDNYPKAVGLAKFAEKHWDKFGRIQLIRLKKGVDGRE
                     HFYRLDMGKTTIRNKVRGITSNEELDRIFDTDAVRED"
     gene            complement(21858..24167)
                     /locus_tag="MJB72_003817"
     CDS             complement(21858..24167)
                     /locus_tag="MJB72_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBY8264846.1"
                     /translation="MAAINDLLRQIPDTSLRSRLEQEFARISKNKKFGLVFEEHIPEC
                     TPLYNVPIKRGSTVALKTGHINDVYTVLNLDGDNALCYNKSTGDTTNIPLADLVSVAQ
                     FGEPIFPTLQPIASVENAPDSSLWHTLIEADNYHALQLLEYLYPKKVDCIYIDPPYNT
                     GARDWKYNNDYVDSSDNWRHSKWLSMMQKRLKIAKRILADDGVLITTIDDNEYAHLWV
                     LLHELFPNLTHTCITIQHNPGGTQGKKFSVTHEYAIFSYSADSTIYRKQHTGGDVYNL
                     RRWGSTSGRYEGATCFYPVILDSNYNIIGFGDLLDKDLHPTSQVEHNEDGTIYVWPID
                     KNGIEKKWRYGRDTVESVRDRMYIERRGDRIEVILRRESEPPKTVWTDPLCNAEAHGT
                     DMIKAILGGGFSYPKSLYAVREALTFAVSGKKDALIVDFFAGSGTTLHAVNLLNAEDN
                     GNRRCILVTNNEVSDDESKTLKKNGFKPGDIEWEKHGICRAITWPRTKYSILGRRDDG
                     SILAGEYFTTQTVSDEVERSFYQLSFVDNPTELTVTAKKQVVSLLRNKEGKSQLPQSL
                     VTKDCKFIVSNKHTASILFDVSAVDEWLSAIEEQDQITDFYIVSKSTATFKSIKTRVS
                     ELLGPIIVTSQVKRPMSDGFPANAEYFKLEFLDKNLVSLGQQFHEILPLLWLKSGAIG
                     KRPEVNYNEEPEMLILPQNSFAILVDETRFSEFVKKLSEESGIETVYFVTNSEEAFRE
                     MTAGVKVNNTYQLYRDYIDNFVLGSRRDS"
     gene            complement(24187..25827)
                     /locus_tag="MJB72_003818"
     CDS             complement(24187..25827)
                     /locus_tag="MJB72_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HBY8264847.1"
                     /translation="MLKEIQCDKFAPDHRVIRFNPGLNTVLGSAGGSNAIGKSTFLWI
                     IDYVFGGESYYSLTDDIKKEIGPHIIYFTFEFDEQPYYFYRSTDDPKNVYRSDEERRF
                     ITKLSLEDYRKFLFQEYNIGLPALTFSEITERFFRIYGRENTLEKYPLLVKPREQDEK
                     AVDFLLKLFGHYKILASIKSMEEELGIKSSQLKSRHRQKVDIEKIESNQKTIESLRKR
                     LQKLMKNSEEAQLALFGFDTQTFERVTAVQKELNSFVRKRNRLQSQLNAIKSNIADSK
                     ADTASEFESLIRFFPNANIKAFEEIEHFHRKIREILGEEMNQEIERLQPLINQYDKEI
                     MRLNRKIEETGIAKEMSERVLSQCVNVSKTIDKLEEETAELIHQKELQEARAEAEQTL
                     EKLFQQQNEKLDEIQDDINLRMDIINGVVTDQQETAPLLYISPHKEIFFETPGNTSEG
                     TAFKSLIVYDLSILELRPVPALIHDSNILKRIEDIHLEHILERYQSSGHQVFIAFDKA
                     DSTTEKAHKILEETAVLRLSDGNELFGRSWSKYESNDY"
     gene            complement(25805..26044)
                     /locus_tag="MJB72_003819"
     CDS             complement(25805..26044)
                     /locus_tag="MJB72_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264848.1"
                     /translation="MRLPNKVIPYTNSVIALFPDILEALTQQDMSPKELFEITAFRKK
                     DIADFLSALNCLFALGKIELIEEGRVLRYVKGNSM"
     gene            complement(26028..27179)
                     /locus_tag="MJB72_003820"
     CDS             complement(26028..27179)
                     /locus_tag="MJB72_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264849.1"
                     /translation="MLLSVAPTYDISGEDGTVSDLILGKKNLSPIVTDTAPGADPHDI
                     SDYFKQSILQMLDSNKRSLIVLALKDIITSDNSIEPDTIVEIVNGMTKQDIMNRNAFV
                     LEEFLAGVFLYTVLYVENRNCESSVSEITDEYIQAFEDQKLDISFIKTYSNLSQDTAY
                     EIAIDAHSLVLLAETGGKCQKCGRVLGIKKEGNDINYAKVVRLSETDEVVLCVECERE
                     IQSASEEEKLALLSDKRDLENLIIARDATSRYTIERQIEQVLREVNLMDVTDDTQLKI
                     EPVKVENKITEKRLKDRVLFDVRQLYEGVNDSLYRLAGENRLNVDKFAKNVRRMYEDA
                     SESHISQSEIYYLLVETLFEKTGRKYRGACEIIISYFVQRCEVFDEITK"
     gene            complement(27290..27508)
                     /locus_tag="MJB72_003821"
     CDS             complement(27290..27508)
                     /locus_tag="MJB72_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264850.1"
                     /translation="MAFSYNKLWKLLIDKKMLKKDLMEKTNLTSTTLAKMGKDLPVSM
                     DVLGRICKALDVNIGDIVDYVDDDTPKK"
     gene            27993..28487
                     /locus_tag="MJB72_003822"
     CDS             27993..28487
                     /locus_tag="MJB72_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8264851.1"
                     /translation="MSRAYKTSQNKRTNYIYYTAEGAKIVITPGEDGVTEAYIQLLHE
                     MDDVEVDEQRRYNYRITAHLDAYHDGEEEAAHDRNKYLADDTANPEQLIIQAEDEAEH
                     QDMLDKLTKAMECLLPQQKELFKKVYLEKRSNTDIATEEGVSEAAIRGRLKKLQERLR
                     KILS"
     gene            28605..28814
                     /locus_tag="MJB72_003823"
     CDS             28605..28814
                     /locus_tag="MJB72_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264852.1"
                     /translation="MNLKHKVTINVARPGGERSSVIQSSRKTIRARMLDFLFGKKVSL
                     LVITPGDSVETVEIKEIKEGGVGHE"
     gene            28807..29124
                     /locus_tag="MJB72_003824"
     CDS             28807..29124
                     /locus_tag="MJB72_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HBY8264853.1"
                     /translation="MSKIKLLLDVVSDLRALADSVQAVADAIASKGPHEITKPEQPKP
                     EKQSEEKQLTLEEVRAVLAEKSHDGFTAEVRALLEKYGASKLSQIDPSKYAALLADAE
                     GLK"
     gene            29121..30248
                     /locus_tag="MJB72_003825"
     CDS             29121..30248
                     /locus_tag="MJB72_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBY8264854.1"
                     /translation="MSKHAILSASGAHRWLNCTPSARLELEFDDNSGEAAAEGTAAHA
                     LSEHKLRKALKMRSKKPVSPYDSDEMDNYTDGYVEFVLEVIEQAKQACSDPLILIEQR
                     LDFSKYVPDGFGTGDCIIIADKVLNIIDFKYGQGVLVSAEDNPQMKLYALGALDLFDG
                     IYDIEMVSMTIYQPRRENVSTSTVSKESLYQWAEEVLKPKAELAFAGDGIYCPGEWCQ
                     FCRAAVKCRARAEAKMKLARFEFALPPLLTDEEITDILSSISDLTSWANEIIAYATDA
                     AVNHGKKWPGFKVVEGRSNRKYKDEEAVAQAAKNAGYQDIYRQSLITITEMEKLMGKS
                     KFNEVLGGLVMKPPGKPTLVPISDKRPEMNTSSAKNDFMEV"
     gene            30254..30814
                     /locus_tag="MJB72_003826"
     CDS             30254..30814
                     /locus_tag="MJB72_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBY8264855.1"
                     /translation="MSTTAKRTNPTKVVTGVVRLSYANVWEPKSINGGAEKYSVSLII
                     PKSDTKTLSAINEAVNAAIEEGRGKFGGKIPNKAALKLPLRDGDIDRPDDEAYANSYF
                     INANSITAPQIVDRNVNPILERSEVYSGVYARVSINFYAFNSNGNKGIACGLGNIQKI
                     RDGEPLGGRTNAADDFATDVDDDFLS"
     gene            30811..32745
                     /locus_tag="MJB72_003827"
     CDS             30811..32745
                     /locus_tag="MJB72_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBY8264856.1"
                     /translation="MRTLSIDIETYSSVDLAKSGVYRYAEAPDFEIILFGYSVDAGPV
                     QVVDLASGEAIPQEIQSAILDSKVIKWAFNAQFERVCLSKYFGVWLEPDSWRCTMVWS
                     AYLGLPLSLEGAAIITGVDKKKLTEGKELIRYFSVPCKPTRSNDGRTRNLPEHAPDKW
                     DRFKAYNLRDVEVELSIQEKLQKFPMPEEEWNNYILDQQINDRGIQLDLELVKKAIQC
                     DEKVREELTSRLKDLTDLENPNSVAQMKKWLSENGLETDSLDKASVKELLKDAPDHLS
                     EVLELRQLLAKSSVKKYSAMENAVCADGRARGLLQFYGANRTGRFAGRLIQVQNLPQN
                     HLLDLKQARCLVRGGHYEALELLYDSIPGVLSELIRTAFVPKKGYKFIVADFSAIEAR
                     VIAWIAGETWRNEVFATHGKIYEASASQMFKVPLEEVTKGSPLRQKGKIAELALGYGG
                     SVGALKAMGALDMGLTEEELKPLVYAWRNANPNIVRLWWDVDRAVKEAVTERCRTETH
                     RTRFEYRSGMLLIWLPSGRQLTYVKPRIGINSFGSESVTYEGIGGTKKWERIESYGPK
                     FVENIVQALSRDILCYSMRKLDEKDFDIVMHVHDEVVLEVPIEISVQDICALMGHTPP
                     WAQGLLLRADGFDCNFYKKD"
     gene            32834..33625
                     /locus_tag="MJB72_003828"
     CDS             32834..33625
                     /locus_tag="MJB72_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBY8264857.1"
                     /translation="MNKLTIFNYEGNTVRTVMKDGSPWWVLRDVCSVLEIGNSRDVMA
                     RLDSDEKGVDIIDTPGGKQEVSIINESGLYSVILVSRKPEAKKFKRWVTHEVLPSIRR
                     HGLYATDELLANPDFLIQALQELKAERAKNAELTTTISIQEQQIAEMKPKASYYDVVL
                     NCKDAVSITTIAKDYGKSGRWFNEYLHNLGVQFRQGKIWLLYQKYAQHGYTTTKTHTY
                     LGNDGTMHSKVHTYWTQKGRLFIYELLKDHGILPLIEQESEFEEM"
     gene            33629..34048
                     /locus_tag="MJB72_003829"
     CDS             33629..34048
                     /locus_tag="MJB72_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HBY8264858.1"
                     /translation="MDRYNAEGYPDPTAAEALENIMREEKAKNYKPCVFICSPFAGDI
                     EENLKKARGYLKFAVEQGTIPFAPHLLYPQVLDDSDPEQRKLGLFFGMVWLRKCEELW
                     VFGPYISKGMQAEIDKASKHRMAIRYFTENCEEVQKL"
     gene            34045..36390
                     /locus_tag="MJB72_003830"
     CDS             34045..36390
                     /locus_tag="MJB72_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264859.1"
                     /translation="MKISVGNSRMDKKWKNKDITWEDFISRVKSTIRTTETVSEFRKM
                     SRAQQDSIKDVGGFVGGALREGKRRNGYVLSRSLLTLDMDYGKPGIWDQIDALHDFKC
                     CIYSTHKHTPDAPRLRLIIPLKREVTEDEYPALGRMVAKEIGIDLFDDTTYEPSRLMY
                     WPSTPSDGEFVFKEKDGELLDPDGYLSKYEDWRDTSMWPVSSRQSEVVQRKITKQADP
                     LSKEGVIGAFCRAYNIEEAIEEFLPDVYEPSTMNGRFDYIPADSSAGLVIYDGKFAYS
                     HHATDPACGMLLNAFDLVRVHKFRELDEKVTENTPPSKLPSFRAMTDLALEDERVKEQ
                     FAEERKAQAEKEFIDEDWEKQLEIDKTGTVKNTLRNLILILENDPNLKSIVFNQLSDS
                     LEIKGDVPWPHPSKFWRDADDAQLISYIDTHYGTFSARNYDVAVAKVADDRSYHPIRE
                     FIEALPEWDKVPRVDTLLIDYLGASDNPYVRAVTRKTLCAAISRVLTPGIKFDSMLVL
                     NGPQGVGKSTLIAKLGGDWFSDSLNLSDTKDKTAAEKLQGYWILEIGELAGLKKAEVE
                     TLRSFLSRQNDIYRASFGRRATPHLRQCIFFGTTNAEKGYLRDTTGNRRFWPVKTPGN
                     GTKKSWQLKQDEILQIWAEALTYVKAGEKLYLDASLEKLAKEEQREAMESDEREGLVR
                     EYLDMLLPEDWDNMDLYERRAYINGTEFGESQRVGVWKRKSVSNMEIWCECFGKDRAN
                     LRRVDGNEISAIMASIGGWTGLVKKERIQLYGPQWVYVPKE"
     gene            36648..36929
                     /locus_tag="MJB72_003831"
     CDS             36648..36929
                     /locus_tag="MJB72_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBY8264860.1"
                     /translation="MREKWIEQQLVKAVKDIGGIALKIASPGFDGMPDRLILLPDKKI
                     AFVEVKAPGKTLRPLQEKRKRQLESLGFLVFCLDHIEQIGGILHEIQAS"
     gene            36910..38259
                     /locus_tag="MJB72_003832"
     CDS             36910..38259
                     /locus_tag="MJB72_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY8264861.1"
                     /translation="MKYKPHEYQVYATEYILTHPIAAVLLDMGLGKSVITLTAIFDLT
                     LDSFLVRKVLVIAPLRVARDTWPAELEKWDHLKGLKYTVSVGSEVQRKTALMKRAQVY
                     IINRENVEWLISRSAIPFDFDMVVIDELSSFKSHQAKRFKSLMKVRPKIKRIVGLTGT
                     PSSNGLMDLWAEYRLLDMGQRLGRFIGRYREDFFVPDKRNQQVIFSYKPKPGAEEAIY
                     RLISDITISMKGTDYLKLPELVINEVDVKLSEKEMEILDIMKRDLITTVKGEEITAAN
                     AAALSGKLLQMANGAVYDDQGVVIHIHDRKLDALEDLIEAANGKPVLIAYWFKHDLSR
                     IQKRFEVEVLSTSDSIKRWNDGEIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQNETVVIHHLIAKGTIDERVMKALNDKNNTQSALIDAVKATLKEV"
     gene            38264..38722
                     /locus_tag="MJB72_003833"
     CDS             38264..38722
                     /locus_tag="MJB72_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264862.1"
                     /translation="MNIVWQYLDKRAAAINALKDYSSMKYIIEHTDEDIATLNEEMSS
                     PASPVINGMPSTHDPKAGEKRLIACINEIDVLKERYRQALEYMDWFQPAWDALTEDEQ
                     YVLKEFYLDDEQKQIDAVYNICDHFNIERSSAYNKKNRALQHLALLLYGK"
     gene            38842..39201
                     /locus_tag="MJB72_003834"
     CDS             38842..39201
                     /locus_tag="MJB72_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBY8264863.1"
                     /translation="MPKKPKRPCSYPGCPELTDGRFCPEHTKKEASRYEKYQRDPETR
                     KRYGRAWKRIRDRYITAHPLCEECKRQGKLTPATEVHHILPLARGGTHDESNLMALCT
                     PCHSAITARDGDRWPSR"
     gene            39313..39864
                     /locus_tag="MJB72_003835"
     CDS             39313..39864
                     /locus_tag="MJB72_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBY8264864.1"
                     /translation="MAKDGTNRGGARIGSGQKKKALIDKIAEGNPGKRKLEVIEFKNT
                     AELQGQEMPQPRAMLSAVQKDGKTLVASEIYELTWKWLEERGCAHLVLPQLLERYAMS
                     AARWIQCEEAISEFGFLAKHPTTGNAIQSPYVSMSHNFMSQTNRLWMEIYQIVRENCA
                     TEYSGTNPQDDVMERLLTARRGK"
     gene            39879..41057
                     /locus_tag="MJB72_003836"
     CDS             39879..41057
                     /locus_tag="MJB72_003836"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBY8264865.1"
                     /translation="MSKRYLTAESVCAGHPDKLCDIIADNILDECLRRDKASRVACEV
                     MATKGKIIVAGEISCSEKIDIRSIVKNVLKELGYNPLKFLIYVYVHNQSSDIAAGVNT
                     ALEARNGINEQYGSIGAGDQGTMYGYATKETREMLPLPLVLSHRIVKRLDEARKGKII
                     KGILPDGKAQVTVEYHDDVPVRVKTIVVSVQHEKNKTQEELKSDILNNVLWQCFEDFP
                     FDDETEILINPSGQFVLGGPAADTGLTGRKIMVDTYGGLASHGGGALCGKDPTKVDRS
                     GAYMARYIAKHIVWCDLAEKCEVAISYAIGKANPVAFSINTFGTGTVSDEVLTLAAQE
                     VFNLRPAAIIEKLRLRNIHYSDTAVYGHFNSCLFPWEDVDRYNELKAAVEKYVDREYV
                     "
     gene            41223..41816
                     /locus_tag="MJB72_003837"
     CDS             41223..41816
                     /locus_tag="MJB72_003837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264866.1"
                     /translation="MNYEKVILEMLTRIKDLEEKVDMLQEYQQELQNKDEGEDDITSG
                     EEKKESGRKRTRREIMTILEEKYGYSVRKGNRSEGSGIVASKNGKSYNIKVSYSRSYT
                     DSDEVICSGWHTVFDKEIDNPDFPYFIFVVEGKESEFHYFIFKREDLINEFDDKAYDA
                     NKKLHFYFRVTKDGKPLEVRETEKDMTAYYNNWDIFK"
     gene            41896..43140
                     /locus_tag="MJB72_003838"
     CDS             41896..43140
                     /locus_tag="MJB72_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HBY8264867.1"
                     /translation="MNIDKISIKKLIPADYNPRKDLRPGDPEYEKLKRSFEEFGYVEP
                     IIWNKTTGRVVGGHQRLKVLLSMGMDVVDCVVVEMDEEKEKALNIALNKISGEWDKEK
                     LALLITDLNAADFDVSLTGFDPGELDDLFKDYLKDKIKEDDFDIDSELNKPAISRLGD
                     VWILGRHRMVCGDSTNKETFNTLMDGKVANLVVTDPPYNVNYEGTAGKIKNDNMANDA
                     FYKFLLDAFINIETALATDGSIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLVL
                     GRSPYQWQHEPVLFGWKKKGKHLWYSDRKQSTIWEFDKPKKNADHPTMKPVSLIAYPI
                     MNSSLTNSIVLDPFGGSGSTLIACEQTDRICYTIELDEKYCDVIVKRYIEQVGTDKDV
                     YVIRQKEKIPFSGVATSSDEIC"
     gene            43237..43929
                     /locus_tag="MJB72_003839"
     CDS             43237..43929
                     /locus_tag="MJB72_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HBY8264868.1"
                     /translation="MKIEFNRTGSERKALVTAIGEILGEKPKYKGAPTFVYQIGRFEV
                     NKEGALIFDEGVVGEKATILLDELERQGFSYVKQDCLLAEHLEDKDLLVIEIPKRDFN
                     DKAYSNLEKILESKGALIKKALGVEELPVEQTEESLRFPWFYLDGDAEKVKAYTHFIT
                     ALCDMAKKQKRITATAKEADNEKYAFRCFLLRLGFIGEEYKTSRRILLSKLSGSSAFK
                     SGVPKREEVNVN"
     gene            43926..44165
                     /locus_tag="MJB72_003840"
     CDS             43926..44165
                     /locus_tag="MJB72_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HBY8264869.1"
                     /translation="MKTVHPKIIKQLRSVYTPGTRVVLVKMNDPYTKLEPGARGTVIG
                     VDDIGTIHVNWDSGSSLGVAYGEDSCRKIEEEKNE"
     gene            44158..44343
                     /locus_tag="MJB72_003841"
     CDS             44158..44343
                     /locus_tag="MJB72_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HBY8264870.1"
                     /translation="MNDIIKSQIFAIRDTGETNMFDLPLLTSIALREGYIELIDYLEK
                     NKEAYVHFILTGKVETE"
     gene            44414..46012
                     /locus_tag="MJB72_003842"
     CDS             44414..46012
                     /locus_tag="MJB72_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBY8264871.1"
                     /translation="MRKLKKYKPTIFKADGSVYDKDAADIAVSFINCLKHTKGEWYGQ
                     PFELIDWQEQIIRDVFGIIKPNGYRQFNTAYIEIAKKQGKSELAAAVALLLTCGDFEH
                     GGEVYGCASDRQQASIVFDVAVDMVEQCPALKARIKPVLSQKRLVYKPLGSFYQVLSA
                     EAYTKHGLNVHGVVFDELHAQPNRQLFDVMTHGSGDARKQPLYFLITTAGNDTHSICY
                     EVHQKAKDILEGRKVDPTFYPVVYGADEDDDWTDPKVWAKANPSMGITVDIEKIQIAC
                     ESAKQNPAEENLFRQLRLNQWVKQSIRWMPMEKWDKCAFAINKESLEGRVCYGGLDLS
                     STTDITAFVLVFPPEYDDDKYIILPYFWIPEDNIEQRVRRDHVPYDIWEQQGFLYTTE
                     GNVVHYGYIENFIEELGLKYNIREIAFDRWGAVQMTQNLENLGFTVVPFGQGFKDMSP
                     PTKELMKLTLEEKLAHGGHPVLRWMMDNIFIRTDPAGNIKPDKEKSTERIDGAVATIM
                     ALDRAIRKGGSGNSVYDGRGLLIL"
     gene            46031..47287
                     /locus_tag="MJB72_003843"
     CDS             46031..47287
                     /locus_tag="MJB72_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY8264872.1"
                     /translation="MGLLSNIFKARDKPQNRTVGSNYSFFFGGTTSGKPVNEHTAMQM
                     TAVYSCVRILAEAVAGLPLHLYKYTESGGKEKAISHPLYFLLHDEPNPEMSSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVIALYPLMPNRMSVDRDSSGSLYYSYTRYSDEAPTM
                     NGMTVTLRPSDVLHIPGLGFDGLVGYSPIAMAKNAIGMAIACEEYGAKFFANGAAPGG
                     VLEHPGTIKDPQKVRDSWNAAYQGSSNSHRVAVLEEGMKYQSIGISPEQAQFLETRKF
                     QINEIARIFRVPPHMVGDLEKSSFSNIEQQSLEFVKYTLDPWVIRWEQAISRSLLRPD
                     EKKLYFAKFNVDGLLRGDYVSRMNGYATARQNGWMSANDIRELENLDRIPPELGGDLY
                     LINGNMTKLADAGIFANKEGMEGKPE"
     gene            47284..47964
                     /locus_tag="MJB72_003844"
     CDS             47284..47964
                     /locus_tag="MJB72_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBY8264873.1"
                     /translation="MKKFWNWVRDADTQTRTLYLNGAIAEESWFEDDVTPAAFKEELM
                     SGEGDIVVWINSPGGDCIAASQIYNMLMDYKGNVTVKIDGIAASAASVIAMAGTEVLM
                     SPTSLMMIHNPFTIAIGDSEEMQKAIQMLDEVKESIINAYELKTGLSRTRLSHLMDAE
                     TWLNANKAVELGFADDIMFKPGESSLQDSFVFSRRAVTNSLMNKLQKPVVKQSVEPLY
                     ERLNLLKY"
     gene            47976..49175
                     /locus_tag="MJB72_003845"
     CDS             47976..49175
                     /locus_tag="MJB72_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBY8264874.1"
                     /translation="MSKILELREKRAKAWEAAKAFLDSKRGSDGLVSAEDAATYDKME
                     ADIINLGKEIARLERQEALEAELNKPVNTPLTEKPAIPGMDTKTGRASDEYRKAFWNV
                     MRSKNPRHDALNALSVGTDSEGGYLVPDEFERTLVQTLEEENVFRKLAKIIQTSSGDR
                     KIPVVVTKGTAAWLDEGEEFDESDSVFGQTSIGAYKLGTMIKVSDELLNDSVFDLEKY
                     ISTEFARRIGAKEEEAFLVGDGDGKPTGIFNVTGGAQLGVSAGSATAITADEIIDLVY
                     SLKAPYRKNAVFLMNDATVKAIRKLKDGQGQYLWQPSLTAGTPDTLLNRPVYTSAYAP
                     IIEAGAKTIAFGDFGYYWIADRQGRSFKRLNELFATTGQVGFLASQRVDGKLILPEAI
                     KVLQQKA"
     gene            49191..49409
                     /locus_tag="MJB72_003846"
     CDS             49191..49409
                     /locus_tag="MJB72_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Head fiber protein"
                     /protein_id="HBY8264875.1"
                     /translation="MSYSAKNYTEQGGEKTVIGGELVIEEGAKVTGLPVLENQPASTA
                     ETVEALVTDFNALLSKLKTAGIMNGDTP"
     gene            49427..49735
                     /locus_tag="MJB72_003847"
     CDS             49427..49735
                     /locus_tag="MJB72_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBY8264876.1"
                     /translation="MTLLEKVKANLILEHDRDDELLQMYITTAIAYAESYQHVPEGHY
                     NENTMPPTTEQAVIMLSSHFYESRDGSTGGFFADNVQAGQQVWNTVNLLLRLDRDWKV
                     "
     gene            49739..50074
                     /locus_tag="MJB72_003848"
     CDS             49739..50074
                     /locus_tag="MJB72_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HBY8264877.1"
                     /translation="MSFGKMNTFIDLISVERTKDSEGFGKSKDTILASVRAYKEDRHG
                     NEKWANRATFSEATALFRFRKIPDVEVSTNMVIVCNDGRYEITNVEDVKGRGMYIEAM
                     AKKVVGSSG"
     gene            50067..50498
                     /locus_tag="MJB72_003849"
     CDS             50067..50498
                     /locus_tag="MJB72_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBY8264878.1"
                     /translation="MAKVQVKMPEDFLLKLSKLGDKTDEIIPKVLESGGEIVLEKVRS
                     NLQAIVGSGTKEKSRSTGELISSLGLSPAKVDRNGNFNVKIGFKEPRRSGESNAKIAN
                     IIEYGKSGQPPKPFLKPARRASRKACIDAMKKRFEQEVENL"
     gene            50495..50848
                     /locus_tag="MJB72_003850"
     CDS             50495..50848
                     /locus_tag="MJB72_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264879.1"
                     /translation="MSILNELNLIADLCGIPVETGRFSGVPPDTYLVITPLIDLFEVH
                     ADNTPGYEVQEARLSLFVKGSYTAIKNAIVRTLLGADFTITDRRYIGHEDDTDYHHYA
                     IDVAKPYEFQLEKEE"
     gene            50852..51454
                     /locus_tag="MJB72_003851"
     CDS             50852..51454
                     /locus_tag="MJB72_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY8264880.1"
                     /translation="MATIGLDRLYYAKITEDINGDETYDTPMPLAKAISAELSVELAE
                     ATLYADDGAAEIVKEFKSGTLTLGIDDIGVAAAGDLTGATIDDNHVLISTSEDGGAPV
                     AIGFRAQKANGKYRYFWLYRVKFGIPATNLATKGDSITFSTPTIEGTVLRRNKLDGQG
                     KHPWKAEVNEGDEGVTSTVINSWFNEVYEPTFAASSGIGE"
     gene            51467..51841
                     /locus_tag="MJB72_003852"
     CDS             51467..51841
                     /locus_tag="MJB72_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264881.1"
                     /translation="MDNERSAKINIGGQEYELILTTKATKEIAGRYGGLENLGEKLLK
                     SENFEMALDEIVWLITLMANQSLLIHNLRNPDNKKPLLTQEEVELLTSPLELATYKNA
                     LTEAMFKGTKRNVESEDDSKNV"
     gene            52066..54624
                     /locus_tag="MJB72_003853"
     CDS             52066..54624
                     /locus_tag="MJB72_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBY8264882.1"
                     /translation="MADNFGLKIGVEGEKEFKNALRDINQAFKVLGSEMALVSSQFDK
                     NDKSIQALTARNEVLEKSIDAQKEKIETLRAALDNASTSFGENDRRTQNWQIQLNKAL
                     AELNGMERELENNHKALRDHADATDDSADNMEDAADAANELADNVDDVGDEMDDASKK
                     TSVFGDVLKANLLSEAIIGGVKALGSAIAGIGKAFIGAMKDGVEYNAQMENYTASFTT
                     MLGDEAKAQKLVNDLKKEAAATPFGMQDLAQSAQTLMSFGMSAEEAQKRMKQLGDISQ
                     GDAEKFKSLTLAFAQMSSTGKLTGQDLMQMINAGFNPLEEISRKTGKSIGELKDEMSK
                     GAISADMVAEAFASATSEGGRFYGSMEAQSKTFSGQMATLEDGVASLKGQLAEGLTTM
                     LSGTVLPMVNGWVDELSGAFEKDGVQGLIDAFGGILEEAVQFISEQLPIVVDIASQII
                     ISLVQGLTTALPQITEAAVMLLMTLVNGIIETLPALITAGIQMIGTIISGIAEALPQL
                     IPAAVSAVVQIVQGLLDNLPMVLEAALQLVLGLTQGILDALPVLIAALPAIITGIVNF
                     VIGAIPQIIEAGIQLLTSLIGALPEIITAIVEAIPQIVDGLITAILGSIPLLIDAGVQ
                     LLVALIQNLPLIITTVITAIPKIVSSLVNAIIGSIPQIIQAGIMLLVSLIKNLPAIIV
                     EVVKAVPQIIAALVKGFTGSIWQIAQVGTNLIKGLWQGISDAGAWLWDKISGFFGGVV
                     DKIKNFFGIRSPSTLFAGIGENMGEGIGVGFEKAMNSVSQDMQNAIPTDFDINPNLNM
                     NGAGISSGYGTFGGSLITIQQMIVRSEDDIRKISQELYNLMQTGSRAQGRFITA"
     gene            54638..55411
                     /locus_tag="MJB72_003854"
     CDS             54638..55411
                     /locus_tag="MJB72_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBY8264883.1"
                     /translation="MGFIYNGINSKNMKIKARLTSWQASPALRNAYEVVPGKAGVADF
                     GCDISERIITISCSIYPQKSFADLVSVLDDLAEWLNPMNGLKQLILEDIPNRFFFARL
                     TEQVECERLLRTAGAFELKFICPDPHAYALTDEQFTISSVGTHEIQRITGNTDSEPIY
                     QLKGSISGSTSTYISITTNGEELRVVGALTADEVLVIDSGLVTAKITDANGNTLRNGL
                     PVLDELNFPVLHKGENEITVSAVGATFSELKILAKSRWR"
     gene            55416..57938
                     /locus_tag="MJB72_003855"
     CDS             55416..57938
                     /locus_tag="MJB72_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY8264884.1"
                     /translation="MAIKSILTSQTDFTGEFPVSEKTVALWRFNEAAPDSNNMLTDDS
                     GHNRNFFVSGWSGTSANLLSGRLGRYFRQNIINPTSEKTHLVATNDGSFFSDLGEKIV
                     VGGWINPTTYSVGQTYIPIFNTRQGPGQPIFYVSLFQGRLRLMLYNASGSLIYDQTET
                     PTITLKNNGWYFIASVIEVNNKRVQNLLCDRSDGAVWQSPVRTFTGDLNQSCVADIVM
                     GMHANTYYYAGGFDDWFYEKDSLLTMEDLISYFKSSILANGGDSAADVDALADPGSVM
                     LKATNGVYAQSGQLFTIAAACSLAGTGRVSVTSEYTAGITAISLVETSTSDDLSSWTE
                     WQAVGASGELQSPNREYIRYRITLSTQDTSRTPKLLEIQLHDIPKPPYERLGFARPVV
                     LDTNGAWEAVLENAFDIVVTSEVNGADILEFKLPFHDSKRETLDNEKQVQIVNDVYRI
                     RTITDEKSSDGRVVTQVYAEAAFYDLSFSAEKEPMEFVAETPEVPMRYALLGTGWSLG
                     NVTVSTKRTWQSTEKNALSILRAIQNIHGGDLIFDSANRLVHLLTFGGTDSGALFCYR
                     KNMKSIQRVVDTRSLITRLYAYGKDGITFASINGNKEYVEDYSYSSEVRVGTLDASSI
                     SNPYQLLEFANMRLAQYAKPRISYVLSAMDLSVLTGYEHEAWKLGDIVTVDDRDLKLS
                     VKTRVVRRQYNLQEPWKTVLELSTTLRELGDSSAGWDKAADILSSTDVLDRQELKDLV
                     PFNHLRNSRADDGLTYWLSSGFTVDPNNGVSGTASFKAEGVLGMTKSLSQTVYPASRK
                     SYTFSAQIASENLQKGPNGQVGIEVVIEYEDGSTETRFIDLF"
     gene            57954..58148
                     /locus_tag="MJB72_003856"
     CDS             57954..58148
                     /locus_tag="MJB72_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264885.1"
                     /translation="MAYFSQTAHAITPRGIGKIKSLTIRLFITDCSGEVFFTDMLLQG
                     GSVATGWIGHVSEIKWTLDG"
     gene            58158..58739
                     /locus_tag="MJB72_003857"
     CDS             58158..58739
                     /locus_tag="MJB72_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264886.1"
                     /translation="MSVTFTRFAETIHCKEDKRVVSVTVKLLLGDCTGTVYFTDIQAQ
                     EGDRLTGYTINTETMLQKFREGGVIVPARFYNGVVRSGETVILFNLGSTSAGLDCHIY
                     PNQNMAAGSIQLSQGAGAHKVIFNEAVSPGDTFSLLASTRQCLKNGNPTDKEGFFQYT
                     ASGDSKHVIKLEDRKSARVLFEFQEMQEGSERL"
     gene            58736..61207
                     /locus_tag="MJB72_003858"
     CDS             58736..61207
                     /locus_tag="MJB72_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBY8264887.1"
                     /translation="MIDYLKGKRCMVWSFMGNTRMYQALRDYGDRIDTVGIFTFEVDI
                     TGTITETGTSISSMLTYINRWPHIKWLLTIMNHGTASIFTALRNNTNGAKDKFLTEII
                     RIMNKYPWCAGVDIDLERGGGYENKDAANALFRDIYNSVKAYDSSKLVNICLPGMTGV
                     QGSVGGENWCVYEDLNAYCDTAAIMSYGMAWAGSAPGSVSPRDWLEGIYDYAVRVMTP
                     EKIFLGLPAYGWNWRIHDTPENLGITYRGISNTYYAAQLWMTGGYNFTDDGPPQPMIP
                     IIAYWDDYDKVPWALPHVYDYMEGWDAVSRTYPLLGETYNRRRYLTAYSKQQKTEFGT
                     IYVDRSGGTPDSYTGNVSVSSQMITLGEEGEAEYEFEISSAGIYDVAIRISFPFWDKN
                     SIHVSLDGTSKVFSENRLWWPYWRTTCWLSLVSGVFLSAGTHTVSVSTSVPGVQFYGF
                     RVCSSFSEEPSAGEAEFMLSPRKFKDVNGLMAEPDKGFKLTTEVLRRKPDSALIWYED
                     FRDDNPLPSSYWTALSGEWQVWQNQNDPASRPYSQLDGYGQLAWKYSGFSDVHLRARL
                     AFTPEGSGRAGIFCGNVFCCLNFNTQRIELYNGASLIGSYTTEIVKTASADLRSNPRM
                     YTIEMRIRGNSVRVYSGASNVLRFTATLSGFSGGYAGVRSDGRILCELLRLGDAWTYE
                     PYERFDVTFPDGTTTEYGRLARTGVTWDSEFQVFTVNNDVEEASTRNQDISMDYDFFH
                     SGLLPLVCGNDYSVKIVPKDINVWISRLFLGDADGFSILYYQDVDSLVYWANEAAYRW
                     KLRGIAIWSLGQEDMRLWEALPKQI"
     gene            61286..61693
                     /locus_tag="MJB72_003859"
     CDS             61286..61693
                     /locus_tag="MJB72_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBY8264888.1"
                     /translation="MKEIWNGVQVALTALGGFLGWFLGGFDGFLYALVALTIADYITG
                     VMCAIVDKKLSSEIGFRGIFKKVLIFVLVGIGHMIDTNLIGEGSVLRTAIIFFYCSNE
                     GISMLENAGRLGLPIPEKLKDILVQLHNKGGNE"
     gene            61693..62394
                     /locus_tag="MJB72_003860"
     CDS             61693..62394
                     /locus_tag="MJB72_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY8264889.1"
                     /translation="MNLRKLILTENACYKAGRKIIPKGIMVHSTGANNPYLRRYVGPD
                     DGLLGVNEYNNHWNQDKPGGRQVCVHAFIGKLKDGSIATYQTLPWNHRGWHAGGKAND
                     THIGFEICEDGLTDASYFNAVYKEAVELCVYLCKLYNLTEKDVIGHYEGYQKGIASNH
                     GDPKNWFPKHGKSMDIFRADVKKLLSEGEKPAEQEKKKYYRVQIGAYSVKANAEAQLA
                     KAKKAGFTDSFIKYD"
     gene            62526..62756
                     /locus_tag="MJB72_003861"
     CDS             62526..62756
                     /locus_tag="MJB72_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264890.1"
                     /translation="MQITKITDKQDQISISKKTLLSAEALQREFDYYRAEKLLSKMLE
                     KGLITEVEFNKIGALNRQTFSPFLKEIMPSNR"
     gene            62809..64374
                     /locus_tag="MJB72_003862"
     CDS             62809..64374
                     /locus_tag="MJB72_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8264891.1"
                     /translation="MKKITKIEGNTANSFIKPKTRVVAYCRVSTGSDEQLVSLQAQKA
                     HYESYIKANPEWEYAGLYYDEGISGTKKENRSDLLRMLSDCETGRIDLIITKSISRFA
                     RNTTDCLEMVRKLIDLGVHIYFEKENINTGSMESELMLSILSGLAESESISISENTKW
                     AIQRRFQNGTFIISYPPYGYQNIDGQMKVIPKQAEIVKYIFAEVLSGKGTQKVANNLN
                     QKGIPSKRGGRWTATTIRGILTNEKYTGDVILQKTYTDSHFNRHTNYGEKNMYLVENH
                     HEAIISYEDFEAVDAILNQRAKEKGIEKRNSKYLNRYAFSSKIICSECGSTFKRRIHS
                     SGPRKYIAWCCSKHISNITECSMQFIRDDDTKTAFVTMMNKLIFGQKFILRPLLNGLR
                     NQNNAASFRRIEELETKIENNMEQSQMLTDLMAKGYLEPALFNKEKNSLEAERERLLA
                     EKDQLTRSVNGNFAKVDEVDRLLKFATKSKMLTAYEDELFENYVEKIIVFSREEVGFE
                     LKCGITLKERLVN"
     gene            64375..64788
                     /locus_tag="MJB72_003863"
     CDS             64375..64788
                     /locus_tag="MJB72_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8264892.1"
                     /translation="MGHTPYGYRIEDGKAVVDDIAAEKVRELFSGYLAGLSLKGAAKK
                     AGLDCYHATASKMLQNKHYLGDEFYPPIIDEETFEKARVEKRKRAEKLGRIWEPKDEP
                     ERDYPVKFKAKPLVQKYEDPYKQAEYAYSLIESEV"
     gene            64792..66363
                     /locus_tag="MJB72_003864"
     CDS             64792..66363
                     /locus_tag="MJB72_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY8264893.1"
                     /translation="MAVSRNVTVIPAIKRVGNNKNSESKSKIRVAAYCRVSTDSEEQA
                     SSYDIQIEHYTNYIKKNKEWELAGIFADDGITGTNTKKRDEFNRMIEECMAGNIDMII
                     TKSISRFARNTLDCLKYIRQLKDKNVAVFFEKENINTMDSKGEILLTIMASLAQQESQ
                     SLSQNVKLGIQYRYQQGEVQVNHKRFLGYTKDENKQLVIEPKGAEVVKRIYREYLEGA
                     SLLQIARGLETDGILTAAGKAKWRPETLKKILQNEKYIGDALLQKTYTVDFLSKKRVK
                     NNGIVPQYYVENSHEPIIPRELFMQVQEEMVRRANLRGGKGGKKRVYSSKYALSSIVY
                     CGHCGDIYRRVHWNNRGYKSIVWRCVSRLEEKGSECTAPTINEETLQTAVVRAINELL
                     ANKEPFLSTLQKNIDTIFNEESDNDTDELDSKLEELQIELLKQAKSKNDYDNVADEIY
                     RLREMKQNSLVENAEREGKRQRIAEMTAFLNEQSHELEEYDEQLVRRLIEKVTIRDDR
                     IEVEFKSGVKIEEMI"
     gene            66571..67209
                     /locus_tag="MJB72_003865"
     CDS             66571..67209
                     /locus_tag="MJB72_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vat family streptogramin A O-acetyltransferase"
                     /protein_id="HBY8264894.1"
                     /translation="MIGPDKKKLYPNENIKTVCFISNLPKRPNVEIGEYTYYSDNKKT
                     PEKFYDNIEHHYEFLGDKLIIGKFCAIAEGVKFIMNGANHRMDGITTYPFNIFGHGWE
                     KVTPTIEQLPFKGDTVIGNDVWIGQNVTIMPGIKIGDGAIIAANSTVVKSVEPYTIYG
                     GNPAKFIKKRFSDEKIEFLLKLQWWNWSEEEIFNNLEMLTSKAGLEQFMKNS"
     gene            67408..67902
                     /locus_tag="MJB72_003866"
     CDS             67408..67902
                     /locus_tag="MJB72_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_022071788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HBY8264895.1"
                     /translation="MVSKTDAIEIITYAEENDINIWIDGGWGVDALLEEETRVHNDID
                     LFVEESNGKKFIEILKEYGFAEVIEAYTTTSHTVYKDSKGRIIDLHIFKFNEQGYIVF
                     EGEAYPPEVFSGIGKIGDKTVRCIDAENQVLFHLGYEHDENDMHDVKLLCERFNIPVP
                     SEYK"
     gene            complement(67918..68058)
                     /locus_tag="MJB72_003867"
     CDS             complement(67918..68058)
                     /locus_tag="MJB72_003867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264896.1"
                     /translation="MTLSSKSASSSLALLSSLIRSNSSLPNPERESLIERAVSFPPLN
                     SN"
     gene            68268..69146
                     /locus_tag="MJB72_003868"
     CDS             68268..69146
                     /locus_tag="MJB72_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY8264897.1"
                     /translation="MDSLRSMNNALEYIEEHLTEEIDYIEVSKIAYCSEYHFKRMFSF
                     LAGISLSEYIRRRRLTLAALDLKDRDLRIIDVAVKYGYNSADSFSRAFHSLHGILPSE
                     ARSENTQLKAYPRMTFQLSIKGGCEMNYRIVEKGPFKLVGFKKRVPIIFEGVNPEIAK
                     MTELLTMDVINQLKAISNVEPTGIISASTNFSEGRMEEKGELDHYIGVATSSNETADF
                     DVLKIDASTWAVFESIGPFPETLQNVWGRIYSEWFPSSGYEAVEGPEILWNESPDTGN
                     PKYRSEIWIPVKKKDY"
     gene            69743..71806
                     /locus_tag="MJB72_003869"
     CDS             69743..71806
                     /locus_tag="MJB72_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8264898.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            71803..73668
                     /locus_tag="MJB72_003870"
     CDS             71803..73668
                     /locus_tag="MJB72_003870"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY8264899.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            73792..74606
                     /locus_tag="MJB72_003871"
                     /pseudo
     CDS             73792..74606
                     /locus_tag="MJB72_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            74631..75119
                     /locus_tag="MJB72_003872"
     CDS             74631..75119
                     /locus_tag="MJB72_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBY8264900.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            75232..75393
                     /locus_tag="MJB72_003873"
     CDS             75232..75393
                     /locus_tag="MJB72_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264901.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            75633..75860
                     /locus_tag="MJB72_003874"
     CDS             75633..75860
                     /locus_tag="MJB72_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264902.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            75857..>76178
                     /locus_tag="MJB72_003875"
     CDS             75857..>76178
                     /locus_tag="MJB72_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBY8264903.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLI"
BASE COUNT        25930 a        11910 c        16231 g        22107 t
ORIGIN      
        1 acttttataa gcatagaaag aatagcaaaa tgcaatggat agtggcaaga tgccacgata
       61 tgaaaaagat tattcaattg catattgtta aaaaacattc aaatcaagcg atattttgat
      121 taaatataaa gttggcacga taaatgctta acaatatagg tgtaagacaa taaaacaata
      181 aaaaataatt ttaataattt ttaagttatt ttagataaga ttaggaactt tgacatcaga
      241 cagttaatat cagaaaaaag tattttatga gtgatttaat cgaaatttta taattgatac
      301 tattaaggag gaaaacgtta tgcttttaga aggagttaaa gtagtagaac tttcaagttt
      361 catcgcagca ccatgttgtg caaaaatgtt aggtgactgg ggtgcagagg ttattaagat
      421 tgaacctata gaaggtgatg gaataagagt tatgggtgga acatttaaat ctccagcatc
      481 agatgatgaa aaccctatgt ttgaattaga aaatggaaat aaaaagggtg taagtattaa
      541 tgtaaaatca aaagaaggag tagaaatatt acataaatta ttatcagaag cagacatatt
      601 tgtaactaat gttagagttc aagcattaga aaaaatgggt atagcttatg accaaataaa
      661 agataagtat ccaggattaa tattctctca aatattagga tatggtgaaa aaggaccttt
      721 aaaagataaa ccaggatttg actatactgc atacttcgca agaggaggag ttagccaatc
      781 tgttatggaa aaaggaacat ctccagcaaa tacagcagca ggatttggtg accactatgc
      841 aggtctagca ctagcagcag gaagtttagc agcattacat aaaaaagctc aaactggtaa
      901 aggtgagaga gtaacagtaa gtcttttcca tacagctata tatggaatgg gaacaatgat
      961 aacaacagca caatacggaa atgaaatgcc tttatcaaga gaaaatccaa acagcccatt
     1021 aatgactaca tataaatgta aagatggaag atggattcaa ttagctttaa tacaatacaa
     1081 caagtggtta ggcaaattct gtaaggttat aaatagagaa tatatattag aagatgatag
     1141 atataataac atagattcaa tggttaatca tgttgaagat ttagttaaga tagttggaga
     1201 agctatgtta gaaaaaacat tagacgagtg gtcagcttta ttagaagaag cagacttacc
     1261 atttgaaaaa attcaaagct gtgaagattt attagatgac gaacaagctt gggcaaatga
     1321 cttcttattt aagaaaacat acgatagcgg aaatacaggt gtcttagtta atactccagt
     1381 tatgtttaga aatgaaggaa ttaaagaata tacaccagca ccaaaagtag gtcaacatac
     1441 tgtagaagta ttaaaatctt taggctacga tgaagagaaa ataaataact ttaaagatag
     1501 taaagttgta agatattaat tagtaattct agatttttta aaaggagaat gctaaagatg
     1561 tacacaatgg gattagatat aggttcaact gcatcaaagg gagtaatctt aaagaatggg
     1621 gaagatattg tagcttctga aacaatatcc tctggtactg ggactactgg accatcaaga
     1681 gttttagaaa aattatatgg caagacaggt cttgcaagag aagatattaa aaaagttgta
     1741 gttacaggat atggaagaat gaactattca gatgctgata agcaaataag tgaattaagc
     1801 tgtcatgcta gaggggtaaa tttcataatt ccagagacaa gaaccattat tgacataggt
     1861 ggtcaagatg caaaggtatt aaaattagat aataatggaa gactattaaa ctttcttatg
     1921 aatgacaaat gtgctgcagg tacaggaaga tttttagatg taatggcaaa aataatagag
     1981 gttgatgtat ctgaactcgg aagtatatct atgaattctc aaaatgaagt atcaataagc
     2041 agtacatgta cagtatttgc agagtctgag gttatatcac atttatctga aaatgcaaaa
     2101 attgaagata tagtggcagg tattcatact tcagtagcaa agagagtttc tagcctagta
     2161 aaaagaatag gagtacaaag aaatgtagtt atggttggtg gagttgctag aaatagtggt
     2221 attgtaagag ctatggcaag agaaatcaac acagaaatta ttgtacctga tatacctcaa
     2281 ttaactggtg ctttaggagc agcgttatat gcttttgatg aagcaaaaga atcacaaaaa
     2341 gaagtgaaaa atatataaaa agagggatga aaatgtctga aaaaaaagaa gctagagtag
     2401 taattaatga tttattagct gaacaatatg caaatgcatt taaagctaaa gaagaaggaa
     2461 gacctgtagg ttggtcaaca tcagtatttc ctcaagagtt agcagaagta tttgacttaa
     2521 acgtattata tccagaaaac caagcagctg gagtagcagc taaaaaaggt tctttagaat
     2581 tatgtgaaat agctgaatct aaaggatatt ctattgacct atgtgcatat gcaagaacaa
     2641 attttggtct tttagaaaat ggtggatgtg aagctttgga tatgccagct ccagatttcc
     2701 tactttgctg taacaatata tgtaaccaag ttataaaatg gtatgaaaat atttcaagag
     2761 aattagatat acctttaata atgattgata caactttcaa taatgaagac gaagttactc
     2821 aatcaagaat agattatatt aaagctcaat ttgaagaagc tataaaacaa ctagaaatta
     2881 tatcaggaaa gaaatttgac cctaagaagt ttgaagaagt aatgaaaata tcagctgaaa
     2941 acggaagact atggaagtat tctatgagtt taccagcaga ttcttctcct tctccaatga
     3001 atggatttga cttatttact tacatggctg taatagtttg tgctagaggt aaaaaagaaa
     3061 ctacagaagc atttaagtta cttatagaag aattagagga caacatgaaa actggtaaat
     3121 cttctttcag aggggaagaa aaatacagaa taatgatgga aggtatacct tgttggccat
     3181 atataggata caagatgaaa acattagcta aatttggagt taacatgaca ggtagtgttt
     3241 acccacatgc ttgggcatta caatatgaag ttaatgattt agatggaatg gcagtagcat
     3301 atagtactat gtttaacaat gtaaacctag accgtatgac aaaatataga gttgattctt
     3361 tagtagaggg taaatgtgat ggagcattct atcatatgaa cagaagctgt aaacttatga
     3421 gtttaataca atatgaaatg caaagaagag cagctgaaga aactggatta ccatatgctg
     3481 gatttgatgg tgaccaagca gaccctagag ctttcactaa tgctcaattt gaaacaagaa
     3541 ttcaaggttt agttgaagta atggaagaaa gaaaaaaact taatagaggt gagatataat
     3601 ggaagctatt ttatctaaaa tgaaagaagt agttgaaaat ccaaatgcgg ctgtaaaaaa
     3661 atataaaagt gaaactggta aaaaagctat aggttgtttc ccagtttatt gcccagaaga
     3721 aattatacat gcagctggaa tgcttccagt tggtatatgg ggagggcaaa cagaattaga
     3781 tttagctaaa caatatttcc ctgcatttgc atgttcaata atgcaatcat gtttagaata
     3841 tggattaaaa ggtgcttatg atgaattatc tggagttatt ataccaggta tgtgtgatac
     3901 actaatttgt ttaggacaaa actggaaatc agcagtacct catataaaat atatatcatt
     3961 agtacaccca caaaatagaa aacttgaagc tggtgtaaaa tacttaatca gtgagtacaa
     4021 aggcgtaaaa agagaacttg aagaaatttg tggatatgaa atagaagaag caaaaattca
     4081 tgaaagtata gaagtttaca atgaacatag aaaaactatg agagactttg ttgaagtagc
     4141 ttataaacat tctaatacta taaaaccatc aataagaagc ttagtaatta agagtgggtt
     4201 ctttatgaga aaagaagaac atactgagct agtgaaagat ttaatagcaa aattaaatgc
     4261 tatgccagaa gaagtctgtt ctggaaagaa agttttatta acaggtatat tagctgattc
     4321 taaagatata ttagacattt tagaagacaa caatatatca gttgtagctg acgacttagc
     4381 acaagaaaca agacaattca gaacagatgt accagcaggt gatgatgcgt tagaaagatt
     4441 agcaagacaa tggtcaaaca tagaaggatg ttcattagct tatgacccta agaaaaaacg
     4501 tgggtcactt atagtagatg aagttaaaaa gaaagatata gatggtgtta tcttctgtat
     4561 gatgaaattc tgtgacccag aagaatacga ttatccttta gttagaaaag atatagaaga
     4621 tagtggaata cctactttat atgttgaaat cgaccaacaa actcagaata atgaacaagc
     4681 cagaactcgt attcaaactt ttgctgagat gatgagttta gcgtaagttc attaaaataa
     4741 atttactata aaatatgaaa attggaggaa gctatgttat ataacaaaga acaagaactt
     4801 ttaagaaaag cagtaagaga ttttgttagt aaagaattag atactttacc agcagaaatg
     4861 gataaaacag gtgtaatgcc taaggaatta atcaaaaaat tagctgatgc aaaatttata
     4921 agcagtaata tcccagaaga atatggtggt gggggagcag gatatgtttc ttacgctata
     4981 gtaatggaag aaatagctag aagatgtgct tcaacagcta cttttgttac agctggttct
     5041 tctcttgctt cattaccaat actttacaat ggtactgaag agcaaaaaca aaaatactta
     5101 aaaggtatag caacaggtga attaatagga gcatttggtt taactgagcc aggagctggt
     5161 tctgatgcag gtggacaaca aacaacagca gaattagttg gagaccacta tatattaaat
     5221 ggtagaaaaa ctttcataac taatggacca ttctgtgatg tagctatagt aatagcagtt
     5281 acagatagaa gtaaaggact tagaggaaca tcagcgttta tagtagaaag taaatgggat
     5341 ggtttctcaa caggagctca tgaagataag atgggtataa gaggaactga aacttctgac
     5401 ttaatatttg aaaacgttaa agttccaaaa gaaaacttac ttggaaaaga aggtcaagga
     5461 tttaagatag caatgggtac tctagaagtt ggtagaatag gtgttgctgc cttagctcta
     5521 ggaatagctc aaggtgcttt agatgaagct gttaaatata caaaacaaag agttcaattt
     5581 ggtaagccta tagctaaatt ccaaaatact caatttacta tagctgacat ggaaacaaaa
     5641 gtttgtgcag ctagaggatt agtttatgat gcagcacaaa agagagatgc aggaatgaga
     5701 gttgctcaag aatctgctat ggctaaatac tatgcatcag aaattgcaaa tgaagttgct
     5761 tataaagcat tacaacttca cggtggatat ggatttataa aagattatga aatcgaaaga
     5821 atgtacagag atgctagaat cgtatcaata tacgaaggaa catcagaagt tcaaaaaatg
     5881 gtaatttcat caaacgtatt aaaataataa ataatctaat gaaattggag gtatagactt
     5941 tgaaaatatt agtttgcgta aaacaagttc cagatactaa cgaagtaaga ataaataaag
     6001 aaacaggtac tcttataaga gatggagttc caagtatatt aaacccagat gatgcaaatg
     6061 cattagagga agctttaaaa attaaagatg agcatgatga tgtaactata acagttataa
     6121 caatgggacc tcctcaagca gactttatgc taagagagtg tttagctatg ggtgctgatg
     6181 atgcaatatt attaagtgat agagcatttg gaggagctga tacttgggct acatcaaata
     6241 ctattgcagc gggaatatct aaagtaggtg actatgacat aatatttgca ggaagacaag
     6301 caatagatgg agatactgca caagttggac cacaaattgc agagaaatta gacattcctc
     6361 aagttacata tgtacaagat ttcaaaatag aaggaaaaga tataatagtt caaagacaat
     6421 tagaagatgg atacgaatta ataaaagtaa gtacacctgt acttttaaca gcagtaaaag
     6481 agttaaatac acctagatac atgtctgttg ataaaatagt aaaagcatat aaacatgatg
     6541 taaaagtttg gacaattgat gatttagatg taaataaaga agaagttggt ttaaaagcat
     6601 caccaactaa agtatttaga tcatttactc ctgaaccaag aggaaaagga gaaatcttag
     6661 aaggtaaagc tgatgaaata gctagtaaaa taataatcgg tctaaaacaa aagcacatta
     6721 tataagttgg aggagtacaa actatgaatg atataaaaga tttaagttct tataaaaacg
     6781 tatggatatt tgcagaacaa agagaaggaa aaatagctcc agtagttata gaattattag
     6841 gagaaggaag aaaattagct aaagaagtag atgcagaact ttgtgcaata ttattaggaa
     6901 aagatgttga tggattagct aaagaattaa tcacttttgg agctgacaaa gtttatgttg
     6961 cagatgatgc tcttttagaa aaatatacaa ctgatgcata tacaaaagta ataaaagatg
     7021 caatagatga aataaaacca gaaataatgc ttttcggagc aactcatata ggtagagact
     7081 tagcacctag aatagcttca agagttggaa ctggattaac agctgactgt actaagttag
     7141 aaatagaccc agaagataag aaaataaaac aaactcgtcc agcatttggt ggaaacataa
     7201 tggctacaat catttgtcca aaccatagac ctcaaatgtc tacagttaga cctggtgtta
     7261 tggataaagc tgaaaaagac gaaacaagaa ctggtgaagt tatagcatta gactacaaaa
     7321 taactcaaga tgatataaga actactgttt tagaaacagt taaaactaag aaagatttag
     7381 tatctcttac agatgcaaat gttatagtat caggtggtct aggattaggt ggaccagaag
     7441 gatttgaaat gcttaagaaa ttagctgaca aattaggtgg agtagttggt tcttctcgtg
     7501 cggctgttga tgctggatgg atagaccatt ctcaccaagt aggtcaaaca ggaactactg
     7561 ttaaaccaaa cctatatata gcttgtggta tatcaggagc aatacaacat ttagcaggta
     7621 tgcaatcatc agatttcata attgctataa acaaaaaccc agcagctcca attttagaaa
     7681 tcgctgacta tggagtagtt ggagacttac atgaaatagt tccaatgctt atagaaaaat
     7741 tagatagcgt tgatgattta ttagaagcta taaaagctta atagatacaa actatataaa
     7801 tattactgat tgaataagtc ttattagtta gtaacataat tattaaccct ttgataagtt
     7861 attgctagtg tgtatttgtc agttattaaa aagtctgttt gaataaaatt ttataataca
     7921 agataatctg gataatcctt atatgtatat tgcttacaaa ttgtgagtta tattctgtaa
     7981 ggattatctt tgttttttta aataaatctt aaatttaatt aaattgtatt aaatagacaa
     8041 ataattacta aatatgtgat aaaatacaat taaaagcaaa atgaaatttt taacaaacta
     8101 taatcattaa aattatatct tagttacgtt ttaatgaaat aaactacttg aaaagacgaa
     8161 tattataagg agttgattat atgtgctgat ggtatttatt gtaagcaaac aataaatttt
     8221 gtatgtagtt caataaatta cttaatgaaa ggtgcgaata ttaatggata ataatttaat
     8281 agggcttata gaacatgtag aaaatccagc cattttatgt aaagaatcag gcgaaataat
     8341 atactgtaat catctaatag atagtatttt tagttttcta gatataaaaa agcctagaaa
     8401 tataaatgaa ttagattcaa attttgacaa aacagaaata ctgacagata gtaaaaagaa
     8461 aatagctttc agagagttaa ggatgactgc acatatatac aatatgaaag ataataataa
     8521 tgaaaataat atagtttatt tatttgagaa gtctctaata tctgataaag taattgaaga
     8581 tataatagag catatagatg aagttgttgt tgtgtttaac aaagatgggg taattgaaaa
     8641 gatgaacact gtcagtgatg agatattacc ttttaaaaga actgaggtac ttggtagaaa
     8701 tataacagac cttgtgagac aaggattagt tgaagaacca ataatattaa acatgcttaa
     8761 agtgaagaaa aagatttata gaaatatcgt ttacccagat gggaagctta ttgcatatac
     8821 ggcagttcca agatgggatt ctaagggaaa gcttacagga ggagtactaa cagggagaga
     8881 tatttcaagg gttattaagc ttgaatctca gataaaatat agtgatatat cagaagacac
     8941 agagtatata agtcaaagta agattatgga caatataaaa aaagttgtta agagagctgc
     9001 agcctcagat tcttctatat ttataaatgg agaatcaggt gtaggaaaag aaataatagc
     9061 aagaacaata tataagtata gttcaaggag agacaaacct tttatagcta taaactgtgg
     9121 agctatacca aatgaattat tagagtcaga gttttttgga tatgaagaag gttcttttac
     9181 tggagctaag aaaaaaggta aaaagggact ttttgaagag gcgaatggtg gaactatttt
     9241 cttggatgag ataggtgagt taccaatgca gatgcaaaaa aaattactta gggttataca
     9301 agaaaatact attactagaa taggtggtag taagcctata aaaatagatg ttagatatat
     9361 aagtgctact aatatttctc atgaagacct tagaaataac cttaaattta gacaagattt
     9421 gtactataga ttaagtgtta ttccagtaaa aatacctcct cttagagaaa gaaaagagga
     9481 tatagtacca ttagtaaatt atttcttaaa actttacaat gaaaagtaca atagagaagt
     9541 agaagtttcg cctaaagtta ttgagttatt agaagaatat tcttggccag gaaacataag
     9601 agaattaaaa aacatcattg aaagatttgt agtattatca gctaaaaatg taattggaga
     9661 agatgaattt aacatgctga taaatttaga tatgattgat aatgaaacag atgatttatc
     9721 accaatagtg gtaaatggaa ttatgaatct taatgatgca tataaaatag tggaccaaat
     9781 aatgataagt aaggctataa ataagtatgg ttcaataaca aaggctgctg aagttatagg
     9841 tatatatcct tctaccatcc atagaaagat aaagagtgga catattcacg tttagctgtc
     9901 agtttaaaaa gacaatataa ttaaaatatt ataagataca taatttatac aacttaaaag
     9961 agatggctta aaaataaatt taattacttt aagtcatctc ttttctttta taaatattta
    10021 aaagttatat tagtttatag agttagcaga acctaaaaca tcatctatct tgctttcaac
    10081 tacagcttgt atagcatctc taccagcacc aatgtatttt cttgggtcaa attcttttgg
    10141 attttcagct aagaattttc tgatagcagc agtcatagct agtcttaagt cagtatccat
    10201 gtttatttta caaacagcca tagaagatgc ttttcttaac atatctacag gtacaccttt
    10261 agcaccagct atatttccac caaattcatt acatgtagca acagcgtttt ggtcaacagc
    10321 agatgcacca tgaagaacta ttgggaaacc tggtaatttg ctttggattt cttctagtat
    10381 atcgaatctt aatttagctt ctcctttgaa tttgaaagca ccatgagaag ttccaatagc
    10441 tattgctaat gaatcaacac ctgttctttc aacgaactca actgcctcag ctggttgagt
    10501 atacttgtga acatcagaag taacatcatc ttctgttcca gctagaacac caagttctgc
    10561 ttcaacaaca acacctttag agtgtgcata ttcaacagct tctttagtta ttctaacatt
    10621 ttcttcaaag tcaaaatgag aaccatctat cataactgaa gagaaaccag catcaataca
    10681 agttttaata gcatccatat ttggaccatg gtctaagtga agagctacat ctacacctat
    10741 ttcatcagaa gcagccttaa ccatttcaac taatgtatga ggtccagcat atttaacagc
    10801 tgacatagaa gcttgtatca taacataaga atttttagct ttagcagctt ttaaaacacc
    10861 ttgtaattgt tctaagtcac ttatgttaaa tgcacctata gcaaatccac cttcgtaagc
    10921 ttttttaaac atttcttttg tagtaattaa tgccatttta aactcctcct attaactttt
    10981 aaaatttacc catcacttat attataacgt taaatcaaaa aaagtatata gtaaattgta
    11041 ataactataa attacaagta taatgttaaa tagaagacaa taggattaaa taaaataata
    11101 aagaatataa acaatttagg aaaactagct atataatgct aactataagg gtaggaacta
    11161 ccttattagt ttggagatac tgataacaat agttgccttg accaagaagt taccacttca
    11221 aacgttatcg aaaggaatta agtggatgat aatttactaa aaatttaaaa taaaggagat
    11281 aaactatgcc acagataaaa ataagaggaa ttaatgaaaa tgatatatgt aaaataagtg
    11341 aaaaaatgat aaatgattta gttgaagctg taaaatgtcc aagagactac tttgaaatag
    11401 aatgtataaa gtcagttgca ataagagatg gtaaaatagc agatgtatat ccttttgttg
    11461 aagtagcttg gtttgataga ggacaagaag tacaagatat agtagcaaga ataataactg
    11521 atagtataag aaataattta gatgtagaaa gtatagattt agcatttaca gtatttgaaa
    11581 aagaaaaata ttatgagaat ggagagcatt tttaatggct aaaaagcaac gtatagataa
    11641 aattctttca aatttaggtt atggaagtag aagtgaaata aagaagtatt gtaaacaagg
    11701 gtctgtagta gtaaatggaa gtgaagtttc aaatccagga actcaagtag acactgaaaa
    11761 tgatgagata ttatttaatg gagaagaagt tatatataga gaatatatat atttgatgat
    11821 gaataaacct gatggatata tatcagcaac tacagataaa tatgacccaa cagtattaga
    11881 tttaattgat ttatcatact tagcttttga accatttcct gttggaaggt tagataaaga
    11941 cacagaggga ttattagttt taactaatga tggaaagctt tctcatagag ttttatctcc
    12001 taagaagcat gtgcctaaga cttattatgc aaaaattgat ggagtagtta ctgaagaaga
    12061 tgtagaagct tttttagaag gtgtagtgct tgatgacgga tataaaacta tgccatcaca
    12121 gttaaatata ttaaaaagtg atgatgaatc agaaatagag cttacaatac atgaaggtaa
    12181 gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag
    12241 attgtctatg ggcaatttaa aattggatga aagtttagag ttaggtgagt atagagagtt
    12301 aacagatgaa gaagtaaaat tgattgaaga aagataataa agatactata gtaaattgga
    12361 ggaatgtctg tgaagaaaaa agatgttata gaatttgaag tagataaaat ggaatttggt
    12421 ggtacatctt taagccaagt tggagataga gttatccata tgaagggtgg aataagtggt
    12481 caaaaggtga gagctggagt taaaaaagtt agaagtaaga aagctgaagt taagatgata
    12541 gaattattag agaactcacc acttgaaact gaagaaactt gcaagcactt tagagagtgt
    12601 ggaggatgta cactattgtc tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa
    12661 gttatggatt tatttttaaa gcaagattta tttggatttc agtttttagg gatagaagga
    12721 agtccagaaa acaaatatta tagaaataag atggaatata cttttggaga tgaagtaaaa
    12781 aatggacctt tgacattagg tcttcataaa aaaggaaagc atatagatat acaaactgtt
    12841 gaggagtgta tgttggttga tgaagacttc tcaaagatat tagtttctag tgtagagttt
    12901 ttcaatgaaa aaaagcttcc ttattataga acaatgaatc acaaaggata tttgagacat
    12961 cttgtagtta gaaaagggat tcatactaat gagattatgg ttaatatagt aacttcttct
    13021 caagaagatt ttgatatgta tgagtttaaa gatatgcttt tagggattga tttaaaagct
    13081 gagttagtag gtgtacttca tactattaat gatggtttag cagatgcagt ccaatgtgat
    13141 gagttaagag tattgtatgg aagagattat atacaagaag aaattttagg tcttaagttt
    13201 aagatttcac cattttcgtt tttccaaacc aatactaagg gagcagaggt tctttatagt
    13261 attgctaggg attttattgg agattacaat gataaagttg tatttgactt gtatagtggt
    13321 actgggtcta ttggacaggt tatggctggt gctgctaaga aagtgtatgg tattgagata
    13381 gttgaggaag ctgttgttgc tgctaatgag aatgctaagt tgaatggact tactaattgt
    13441 gagtttattg ctggggatgt ggctaaggtt gttaaggatt taaaggacaa gcctgatttg
    13501 attattgttg accctcctag accaggcatt cataaggatg ctattaggga tatttgtgga
    13561 tttggtgcta aggagattgt ttatatttct tgtaatccta agtctttggt tgttgatttg
    13621 gttgatttta aggggtatgg ttatgagatt aagatggtta agtgtatgga tatgttccca
    13681 aatacgccac atgtggagtg cgtggtattg atgtcaagag tggagaaata ggactattca
    13741 aaacactgat aaataaaggg tttccagaca tttggttttc ctcacggtta ctttgccgaa
    13801 ggagataatg taaggaaacc cttatttttt attgtttgtg gaaacatatc aactaccaaa
    13861 aaggaatagg gttgagttga cagattagat aatactaatt ttgcataagt tgaggagaca
    13921 ggatagatgt atacttaatc atctcgtata gctaatgtgt cgaaatatct atcaagttcc
    13981 tcgtttgatg taatgtcatg aactttattc cttattctgt caaaaattta tatcctccac
    14041 taatattata atcgtttatt attacccttt attatcggaa cccaaagttc atatctgtga
    14101 atatcatttt tttctctgaa aaactgaaca ttttctgcaa gttcatatcc gtgatcagga
    14161 aaccactctg taaataattt ataccatgtg tcattaataa cgctgtatga aagagagggg
    14221 agttcaaaaa ctgcccaagt ctgcatgggt atctcgagtt ccacaagaga ttcagggaga
    14281 ggattggaga acttttcact tatctcaact cctacataat agtcatttaa ttcttcccat
    14341 tgtttgacag ctatatatag tatttcacct actcttcttt cggacaattg tttgaaatat
    14401 tgacaaaaat tttcccgagt tgtttggttg atatttggaa caaaaatacc tttttcattt
    14461 ggagttatag tttccctgat gctaacaact ttaaaagttc ctttattttc tagtttgtac
    14521 ttcatgatac atcatccttt ctatttattg agtttattca aatttttatg aatatactat
    14581 tgtttgaaag ctgataccat cctctcttaa accatactag caaaataaaa aggattattc
    14641 tcgataaagt atatacggat attatcggat tctgtgaatg gacgagtaag acattattga
    14701 aattctatta tttaaaagaa agccaaatta tttaaaaatg aaaccagtag aaaacatgat
    14761 tgtaaaacga aaggaaaaat tgctttgttt ctattgatat acatataata atttaatatc
    14821 attaccctgc aaaactcata gacaatatgt cgggtattcc aattttgatt ttgatataat
    14881 ggttgtaaac tatggaatga tgttgtatca taaaagttga caccctattc tcaaggactt
    14941 tgatatataa tcataatcaa tacgagaaca ggagaaacaa cctatggcac aaaatcaaaa
    15001 atcttatgac aatgaattta aagcacaagc tgtaaaactt gctcaggaaa ttggtgggca
    15061 taaagcagct aacgaattag ggattcccaa gggtactatg tacacctgga taaaagcctt
    15121 caaagaaggt cgtctcagcg caaatgacgc ggttcatacg cctaagaatg ccttatctct
    15181 taacgatgag cttatcgaac tcaaaaagcg cattaaagag caggataagg aaatccgtcg
    15241 tttaaaagaa gaaaatgaat ttctcgaaga agccagtgct tttttcgcag ccagccgtcg
    15301 gaagtcagca aaaaacagag actgatgttt attgcaacca aaactgatga cggcgagatt
    15361 aagggcaaaa tttcttttta ttgcagagtg cttcatgttt ccagacaagc attcaataaa
    15421 tacctaaaag caaaggatgc tccatggaaa tatcaggaat tggcagatgc aatgcttgat
    15481 atttgtagtg aggatgaatg taatgacact tatggaagaa tccgtatgta tcaggcatta
    15541 caactcaaac agccggaggg tatacacatt cccggcgaga gaactgttta tcgtgtcatg
    15601 gacgaaattg gtcttaatca caaaccaaaa cgcaagccta atggtattac taaagctgac
    15661 aaagaagccc ggaaatcaga tgatttaata aaacgtgact tcactgctga aaaacctcta
    15721 aaaaaatgta ttactgatat gactgaaata aaagcttctg atggaaaact gtatgtttca
    15781 gccatctttg actgctacga tttagcggta ttgggtcttg ccatggatac aaatatgaaa
    15841 gctccccttt gtgaacaaac cttagataac gcttatagag cctatcctat gcttagggga
    15901 gctattcttc acagtgacag aggtacgcag tataccagcg ggttatatcg taaggcaatc
    15961 aacaaatatg gcattcttca aagcatgaac agtgccggtg gcagatgcca tgacaatgcc
    16021 agatgtgaaa gtatgtgggc aagattcaaa gaggaattac tttatggacg ttacgatacc
    16081 agctcaatga cagtagaaca gttaaaaact cttatttgga gatacttcat cggctattgg
    16141 aataaccggc gaatctgttc agctaacggt ggcttacctc cgatggttaa gcgacagcag
    16201 tactatgatt cattgcagga ggcagcataa ggtcgaacat tcttgagaaa aaagtgtaaa
    16261 ccaatattga caatatcaga actaattaaa tcagagtaat ttaccattaa taaacgaaac
    16321 aatgcaaagt ctaaagaaaa gggctataga aacttattta gaatatatga atgctctcgt
    16381 taatcctaga tattttatta tcatagatta agaaaaagta tggtaattga taaaatttat
    16441 tgtcaggcag ttttactgac atctaacaaa aacatgagtg aggtataaat atgaaaccat
    16501 taaagcaatt aaatctagca atgacttata ttgaggataa tttgtcaaaa gaaatagact
    16561 ttcaaatggt tgcacaactt gcttgttgct cagagtatca cttcagaaga ttgttttctt
    16621 ttctaagcgg aatgacattg agtgaatata ttcgacaaag gcgattatct caggccgctc
    16681 ttgagttatg caacgctgac gtaaagatta ttgatgtagc tatgaagtat ggttatagtt
    16741 caccggacgc ctttacaaga gcctttcagg ggttccatgg cgtcactcca agagaggcaa
    16801 aaacaattgg tactgctttg aaagctatcc ctccaatgac attccaactg acaataaaag
    16861 gaggaagcaa gatggattat cgcattgttg aaaaagaagc tttttatata gtagggataa
    16921 aaagaaggat tacattgatc tatcgaggag ttaatcctga tgttaaggca atgtatttaa
    16981 gcctttcaaa agaagatttt aggaaattgg ctcaactgtc taatatggaa ctagagggca
    17041 tagtaaacgc atctgtaaat tttgaggttg atagatttga gggaagttca ttggatcatt
    17101 tcattggagt agcaacaact ataaagcctg aaggaaagtg ggatgtgctt tccgttccag
    17161 cttctacatg ggctgttttt actgtagaag gtactttccc ggaagcatta caaactacat
    17221 gggcaaaaat tgcatctgaa tggttagtac cgcaagtgtc tggatatgaa cttaacgaag
    17281 gaccagagtt gctctgctac aataaagatg agtatctaga ttcatcaaac ttccgatgtg
    17341 aaatctggat tcctgtagtt aagaaataag gtgtgttagt tatatgcaat aatgaaatta
    17401 ttaaaaaata atatttggtg taaataaaag agaggtgtgc ttactatatg aaatataaat
    17461 ttgaaacaat taagaaagat tatagcgatt tctcaagtgg acgtgtttta tataatgcag
    17521 caaacacaac agcatttccg gttaggttag caagtgaggt tattcagaga gctttccata
    17581 tccttgataa agatggagcc agtggtccat ataaaatata tgatccatgt tgtgggggag
    17641 ggtttttgct tacaaccatt ggatttctat atcataattt gatatctgaa cttatagcaa
    17701 ctgattatga tgttgaggtg ttagagatag caaagaaaaa cctttctttg ctttcagaag
    17761 agggattaga tagaagaatg aaggaaatta aagggtatat tgaagtattt agaaaagatt
    17821 ctcatataca ggcattgaaa agtgtaaatc aattgaaaac cctgatagga gaagcaaata
    17881 tcaatattca ttgtgctcaa cgcgatatta ctgatatgag tgcctttccg attaaagaag
    17941 taaatattat cattactgat ataccttatg gaattaacgt agaatggaat agtggtagta
    18001 ataatccaat acaaaatcta ttcgaaaata gctataaggc ccttgacaaa aatcactcac
    18061 tgatggtagt gatagctgat aagaaatcaa aacttgaaca taaattattt aaacgggtag
    18121 agtattttaa gcttggtaaa cggcaagttg ctttttttaa accaaatagg tgctgagttg
    18181 gcttttaaaa tgtgttggaa aaacttgatg aaaatccaaa aaattacagt taacaaatat
    18241 atttggaaag aaaatgctga attaaagtgc aaattttgaa ataaaaggag ttattaaaat
    18301 atgataaaaa atagagtggt aaagataagt aaagaagata atgattttca acactttgaa
    18361 gtcattaaaa gaaaccgaaa caaacgacat aaacataaag aattttttgt tgaaggtgtg
    18421 agaaatatca acgaagccat taggaataaa tgggagataa gctcttttat ttactcagga
    18481 gaaaatgaat tatcgggttg ggctaagagt atactaagca acgttaaggt aagttttcaa
    18541 tatgaattac ctaatcactt aatggataaa ttgagcagaa aaatggatac atcagaatta
    18601 ttagctattg taaaaatgaa aactaatgat ttaagcagaa taaaactaac taaaaaaaac
    18661 ccattaattg ttgtaatgga tagaccatca aacaagggaa acttgggaac cttaatacgt
    18721 tcttgcgatg cacttggcgc taatggatta ataataaccg gacatgctgt tgacttatat
    18781 gacccagaga caatagcttc atcggttggt acatttttta gttttccggt gttaaattta
    18841 ccttctcata aagagttgta tgagtggatt caatcattaa aaagtcaaca tccaaacttt
    18901 caaattattg gaacaagtgc tcatggtaat aaaataatta atcagtgtga tttcctaaaa
    18961 cctacagtat tacttttggg aaatgaaaca cttggtctta gtagaaatta taaagaaata
    19021 tctgatttat tggtggagat accaatggat ggagtggcat catctatgaa tgtttcttgt
    19081 gcagcatcga tagtactata tgaaataaaa agacaaagaa gtttataaga gtcttatagg
    19141 gtagagaaat aagagtattc aaaacactga taaataaagg gtttcctgac aatgaatttt
    19201 tctcatgaca tctttgtcga ggacgttaat gtcgggaaac ccttattttt attcttcgag
    19261 gaaacatatc aactaccctg aaagtgatag ggttgagttg acaggttaga taaataaaca
    19321 gggttgagaa gacaggatag atgtattatt tagtcctctc ttacagcgtc tgtatcgaag
    19381 attctatcaa gctcctcgtt tgatgtaatg ccacgaactt tattcctaat cgttgttttg
    19441 cccatatcta atcgatagaa atgttcgcgt ccatcaacac ccttttttaa tctaataagc
    19501 tgtattctgc caaatttatc ccagtgcttc tctgcgaatt ttgctaaacc tactgcctta
    19561 gggtaattgt ctttgcggct aggatcgtgt ggttctagaa tatcaaaaac ataaccttgt
    19621 gcgtcagatc ttacaatcac taaatcaggg aacatagaag tagtgacacc gcccacttcg
    19681 taaggaattt caagtgacca ttttttgcgg tcaagattac gtaaccaaca tacagcacca
    19741 tttttgagtt cttctgtaac aactcctatt tcccatgtgt ttagtgatgt ttcgaatgtg
    19801 ccatcctcag aacaataaag gtgctgctca aacttcatgc tattttctgg tacagaaaaa
    19861 tcgattgaat ttggtaatat ccatggtaca gatatcggtt gtacagaagc attagttaat
    19921 ctttcataga cattctttcg tgcctcgcta agtctagcaa tggatctctt gttgttttcg
    19981 tacaatgata taaattcttt ctcagcataa tcattaatcc tttccattgc atctgtatca
    20041 tttgtaagaa tgatgatttc tttttttaca tcaatatggt cacgggtact atgacgaatc
    20101 caatattctt tgtgtaagcc ctctcctaac aactttccag cttgctcaaa atgtcgggaa
    20161 atatcaaact cagacatggt catagtctgt gtttcttcat caaaggagta agcattatca
    20221 ccataatcaa atatcagtgt accgagagca aaaccagtaa tcgacgcccc tcgagtatca
    20281 tagtctccac tttcttttat tcgtaaaatc tcatcatcca ttttagataa aactgcattt
    20341 ttaacagcct tttgtgctcc taagtcaata ccatccatcg tcagcgcacg agagagctgt
    20401 attaataatt taagtggtgc ttgctttcga gatgaatcta tccgataagt tatgaggtta
    20461 tccattgcct caaatacatc agaataggca agatttcgtc caagtgtaac aagttcttta
    20521 ctggtccctg cttcggtagg catagcagcc tcactatcac gtagagcatt aactacattc
    20581 ttcacggttt cttcatcaaa gtatggaaga aataagctga cactattaag ctcagcatcg
    20641 gaagaaattc ttcttgccaa aggtgtacgt atcatacgtc ccagcaattg agcaatgtaa
    20701 gtataatctt gggcactgcg gaatgacatc atagtttcag ctcgtgggca gtcccagcct
    20761 gtggaaagat tcattttgaa aaacacgacc aacacatttt cttcgtcttg aattcgagat
    20821 gcttcaattt gctgaatttc aacatcgcgt actttaagcg taccgcggtc attaaaagta
    20881 tgtaccgctt ctccaggctg taatttgcgt ccaagagttt cttctagtaa atcgatgcaa
    20941 gctcccaaat ccgtttgggt tgctatacga tcatttccat cttcaacttg aataacaaga
    21001 atagggttta ccattttttc atcttcgcgt tcacagtaag tcttccagtg aatgcatttt
    21061 ttgcgccaat tatcaaccgc tcctttgaac atggtcatat cagcatttaa ttgaatatct
    21121 gggtagtgaa ttatgattct atctttcaaa agcccagact cacggacatg ctctggggga
    21181 acgatgactt tttgaactgt tgatgtcgtt ccagcaatta agctatcaaa tctttgagga
    21241 gttgcagtca cccctataac taaaggcata acgcaaagac catcatcctc actaccctta
    21301 atgaattttt gcatgataga ctgcgcctta ttttctgctt gcatagaggt ataagttccc
    21361 ctatgtgctt catctattac cacataaaac ttttttggaa ttcgtttggc agtattagta
    21421 agggtctccc atatagaata ctgccggtta tcagatgtac ctgtcagcag tttatctgag
    21481 ccaagttttt gagtgttcaa aaagtaaatg cagccaccct caaaatactc tgcattgaag
    21541 gttgagtcta ttgtcactaa atcacggaca cggattttat cggatttact ttcgattttc
    21601 aaccttgttt gctcgttaag ctcaggtgaa tccgaaagcc aaatgaaaac agaatctggt
    21661 tctccaatgt tgtccgcatt tccatataaa atatcttcaa aaagagttgt cataattatc
    21721 gttttacctg aacccgtagg cgcagaaaag gaaattatct gcgggtcgcg ttcactccac
    21781 attaaatgcg ccttgttgat tttatcatgt aactcagaaa gtgccgtctc ctgaaaagga
    21841 aataatatat ctctcattta tgaatccctc ctgcttccta acacaaagtt atcaatataa
    21901 tctcgatata attgatatgt gttattaact tttactccgg cagtcatttc tcgaaatgct
    21961 tcctcggagt tagttacaaa atagaccgtt tcgataccgc tttcttcaga aagttttttg
    22021 acaaattctg aaaatcgtgt ttcatcaact aaaatagcaa agctattttg tggaaggatc
    22081 aacatttctg gctcttcatt gtaatttact tctggtcgtt ttccaatagc tccagatttg
    22141 agccaaagta atggtagtat ttcatggaat tgttggccca atgaaacaag atttttatct
    22201 aggaattcta acttgaagta ttcagcattt gcaggaaaac catcgctcat tggacgctta
    22261 acttgtgatg ttacaataat ggggcctagc aattctgaaa cacgtgtctt aattgactta
    22321 aatgtagctg tcgattttga aactatataa aagtctgtaa tttgatcctg ttcttcaatg
    22381 gcagataacc actcatcaac tgcacttaca tcaaaaagaa ttgatgccgt atgtttattc
    22441 gaaacaatga atttacaatc tttggttact agtgattgtg gcaattgtga ctttccttcc
    22501 ttatttcgaa gtaaagaaac aacttgcttc ttggcagtta ctgttaactc tgtagggtta
    22561 tctacaaaac ttaactgata gaatgaacgc tcaacttcat cagatacagt ttgagttgtg
    22621 aagtactcac ccgctaagat agagccatca tcacgtcttc caaggatact atactttgtt
    22681 ctaggccaag ttatggcccg gcaaattcca tgtttttccc actcaatatc accaggctta
    22741 aagccatttt ttttcagagt tttcgattca tcatctgaga cctcgttatt agttactaat
    22801 atacagcgac ggtttccgtt gtcctcagca ttaagtaagt ttactgcgtg taatgtggta
    22861 ccgctacctg caaagaagtc aacaatcaag gcatctttct taccagaaac tgcgaatgtc
    22921 agtgcttcgc ggaccgcata gagtgattta gggtatgaga agccgccacc aagtatggcc
    22981 ttgatcatat cagtaccgtg ggcttctgca ttacataacg gatcagtcca gacagtttta
    23041 ggaggttcac tttctctacg aaggattact tctattcggt ctcctcgcct ttcaatatac
    23101 atcctatctc taacagattc tacagtatct cgtccatatc tccatttctt ttctatgccg
    23161 tttttatcaa ttggccagac ataaatggta ccatcttcgt tgtgttctac ttgtgatgtt
    23221 ggatgtaaat ccttatcaag taaatcaccg aaaccgataa tgttataatt cgagtctaaa
    23281 attacgggat agaaacatgt tgcgccttca taacggcctg aagtacttcc ccaacgtctt
    23341 agattataga catcaccacc agtatgctgt ttacggtaaa tagtactatc agctgaatat
    23401 gaaaatatcg catattcgtg agtcacagag aattttttgc cctgagttcc accgggatta
    23461 tgctgaatgg ttatgcaagt atgagttaag tttggaaaaa gctcatgaag tagaacccat
    23521 agatgcgcat attcattatc atcaatagta gtaatcaaaa caccatcatc agcaagtatt
    23581 ctttttgcga tcttcaatcg tttttgcatc attgagagcc acttactgtg tcgccagtta
    23641 tcactagaat caacatagtc attattgtat ttccaatcac gcgcacctgt gttgtatggg
    23701 gggtcaatat aaatacaatc aaccttctta ggatagagat attccagtaa ctggagggcg
    23761 tggtagttgt ctgcttctat gagcgtatgc cataagctac tatcaggtgc attctccact
    23821 gaagcgatcg gctgcagcgt ggggaatatt ggttcgccaa actgcgcaac cgatactaga
    23881 tctgcaagtg gaatgttcgt cgtatcacca gttgatttgt tataacatag tgcattatca
    23941 ccgtctaaat tcaatacggt atatacatca ttaatgtggc cagtttttaa cgcaactgtt
    24001 gaaccgcgtt taataggaac attataaaga ggagtgcatt ctgggatatg ctcctcaaat
    24061 acaagtccaa attttttatt ttttgaaatg cgagcaaatt cttgttccaa gcgactacgc
    24121 aaagaagtgt caggaatttg acgcagtaaa tcatttattg cagccatagt ttttttcctc
    24181 ctatgtttaa taatcattgg attcgtattt actccacgaa cgaccaaata attcatttcc
    24241 gtcagataag cgcaagacag ccgtttcttc taaaattttg tgtgccttct cagttgtaga
    24301 atcggcctta tcgaatgcga taaaaacttg gtgcccacta gattggtaac gttccaatat
    24361 gtgttctaga tggatatctt caatacgttt taggatatta gaatcatgta taagcgcagg
    24421 aacaggacgt aattctaata tactcaagtc gtacacaata agactcttga atgcagttcc
    24481 ctcgcttgtg tttccaggtg tttcaaaaaa gatttcttta tgtggagaga tatacaaaag
    24541 gggagcagtt tcctgttgat cagttactac accgttaatt atatccatac gcaaatttat
    24601 gtcatcttgt atttcatcta gtttttcatt ttgttgctga aacagttttt caagggtttg
    24661 ttctgcttca gctcgtgctt cttgaagctc tttttggtga atcaattctg ctgtttcttc
    24721 ctcaagtttg tcgatagttt tagacacatt aacacactga gagaggaccc tctcagacat
    24781 ctcttttgca ataccagtct cctcaatttt tcgattaagt cgcattattt ctttatcata
    24841 ctgattgatt agcggttgta agcgctctat ttcctgattc atttcttcac caaggatttc
    24901 tctaattttc ctatggaaat gttcaatctc ttcaaatgct ttaatattag catttgggaa
    24961 aaaacgtatt aaggattcaa attcacttgc agtatcggct ttactatcgg caatattgct
    25021 ctttatagca ttaagctgag actgcagacg attacgcttg cgaacaaaac tattcaactc
    25081 tttttgtact gctgttaccc gttcaaacgt ctgagtgtca aatccaaaca atgccaattg
    25141 agcttcttcg ctatttttca tcagcttttg tagccttttc ctcaatgact ctattgtttt
    25201 ttgattactt tcgattttct ctatgtccac cttctggcga tggcgagatt taagttgaga
    25261 agacttaatc ccgagttctt cttccatgct ttttatagaa gcaagaattt tgtaatgtcc
    25321 gaatagtttt aatagaaaat ctacagcctt ttcatcctgc tcacgaggtt tgacaagtag
    25381 cggatacttt tctagtgtgt tttcacggcc atagatacgg aaaaaccgtt cggttatttc
    25441 tgaaaatgta agggcaggaa gcccaatatt atattcttga aacaaaaatt tccgataatc
    25501 ttccagtgat aattttgtaa tgaaacggcg ttcttcatcg ctcctatata catttttagg
    25561 atcatcagta cttctataga aatagtatgg ctgttcatca aactcgaaag taaaatagat
    25621 gatatgcggg cctatttcct tttttatatc atcagttagg gagtagtagc tttcaccacc
    25681 gaatacataa tctataatcc atagaaatgt tgattttccg attgcattgc taccgcctgc
    25741 actacctaaa accgtattta atcctgggtt gaatcgaata actcgatgat ctggagcaaa
    25801 tttatcacat tgaatttcct ttaacatagc gcagtaccct cccttcctca attaattcaa
    25861 ttttacctaa tgcaaataag cagtttaaag cgcttagaaa atctgcgatg tcctttttac
    25921 gaaaagctgt tatttcaaac aattctttag gggacatgtc ctgctgcgtc aatgcctcta
    25981 aaatatccgg gaagagtgca atgacactat tagtataggg aattacttta tttggtaatc
    26041 tcatcgaaca cctcacacct ttgtacgaag taggagatta taatctcaca tgctcctcta
    26101 tatttgcgac ctgtcttttc aaacagtgtt tcaacaagca gatagtaaat ttcgctctgg
    26161 gatatatggg attcacttgc atcttcatac atccgtctaa cgtttttagc aaacttatca
    26221 acgtttaatc tgttttcacc tgctaaccga tataatgaat cgtttacccc ctcatataac
    26281 tgccggacat caaaaagcac ccgatccttc aatcgctttt cggtgatttt attttcaacc
    26341 ttaacaggtt cgatttttag ttgtgtatca tcagtaacat ccattaaatt gacttctcga
    26401 agtacttgtt caatttgtct ttctattgta tatcgtgaag ttgcatctct tgctattata
    26461 agattttcca agtcacgctt gtccgagagc aaagccaatt tttcttcttc ggaggcactc
    26521 tgaatttctc gctcacattc aacacataga accacttcgt cagtttctga aaggcgaacg
    26581 actttagcgt agttaatgtc gttgccttct ttttttatac ctaagactct tccgcacttt
    26641 tggcatttgc cacccgtttc tgcaagcaat acgagagagt gggcatcaat tgcaatttcg
    26701 taggcagtat cctgagacaa attgctatat gtcttaataa agcttatatc taatttttga
    26761 tcttcaaagg cctgtatgta ttcatctgtt atttcactaa cactactctc acaatttcga
    26821 ttttcgacgt aaagcactgt atatagaaag actcctgcga ggaattcctc caatacaaag
    26881 gcattacgat tcatgatatc ttgctttgtc atgccattta ctatttcaac aattgtatca
    26941 ggctcgatgg agttatcgga tgtaataatg tctttcagag ccaaaacaat taaactcctc
    27001 ttattgctat ccaacatctg aagaatactt tgtttaaagt aatctgagat atcatgagga
    27061 tcggcaccgg gagctgtatc agttactatg ggggatagat tttttttgcc aagtattaaa
    27121 tcagaaactg tgccatcttc accactaata tcataagtag gcgcaacaga aagcagcatg
    27181 gtaccacaca atcgcttttg tgtaactctt ttagccatgc atctctttaa aattgttgca
    27241 taagtaccaa agcataactt tttcatacat tgcgccccct taaataattt tatttcttcg
    27301 gagtatcatc atcgacgtag tcaacaatat cgccaatgtt aacatcaaga gctttacaga
    27361 ttcttcctaa tacatccatg cttaccggca aatctttacc catttttgcc aacgttgttg
    27421 aagttaaatt tgttttttcc atcagatctt ttttcaacat ctttttatca attaaaagtt
    27481 tccaaagttt attataacta aatgccattt ttaacgccct ccaaacacta ttggatggtt
    27541 ttattgatat atatatattt accattatag caatttacta agcgtatttc aatcaataat
    27601 ctaatgttac taagtatttc tttacaaatg cttagttttt ttattgtgaa agagtttgcg
    27661 gagtaccagc ggagtatcag cagagtgcca gctgaggatt tcagacgccc aaattattaa
    27721 aatttaaata gatggtaaga caagcttact ttagcagata gaaaaacttg accacttacc
    27781 aactaataaa aaaaatatcg aatgcctgat ttgcaataag ggcagaggat acatattgtt
    27841 tcgctccaat ccgtaaggat tgttgcggtg caatagaagt accttacctt attgcgctca
    27901 ttttcaggat aaaaaggtct gtgtacttct ggtacaggcc tatttttgta tcctttgccg
    27961 ccaatgcaat ccggcggaaa ggatgcaaaa acatgtcaag agcgtacaaa acaagtcaaa
    28021 ataagagaac taactatatt tattacactg cagaaggagc aaagatcgta attaccccag
    28081 gtgaggatgg cgtcaccgaa gcttacattc agcttttaca tgagatggat gacgtcgaag
    28141 tggacgagca acgccggtat aattaccgaa taacagcaca tctggatgcc tatcacgatg
    28201 gcgaagagga agcagcccat gaccgcaata agtatcttgc agatgatact gctaatccag
    28261 aacagctaat tatacaagca gaagatgagg ctgaacatca agatatgctg gacaagttaa
    28321 ccaaggcaat ggagtgtctt ttaccacaac aaaaagaact cttcaaaaaa gtataccttg
    28381 aaaaacgatc caacactgat attgcaacag aagaaggtgt ttcagaagcg gctattcgtg
    28441 gtcgcctcaa gaagcttcaa gaaagactta gaaaaattct atcctaattg ggttcgcaag
    28501 aaccctttat aaaaaaatct tttctgaaaa gggggttcga aagaactctc tttttcgctt
    28561 atcggtgagg ggcagataaa tcgcccccag aaaggagatg gaatatgaac cttaaacaca
    28621 aagtcacaat caatgtggca aggcccggtg gcgaaagaag ctcagtaatt caaagcagtc
    28681 ggaagacaat ccgagcgaga atgcttgatt tcctatttgg aaagaaagtc agtctgcttg
    28741 taattactcc cggtgactca gttgagacgg tagaaataaa ggaaatcaag gaaggaggtg
    28801 tgggtcatga gtaagattaa gctccttctc gatgtggtgt cagatcttag agctctggca
    28861 gatagtgttc aggctgtagc tgatgcaata gcaagtaaag ggcctcatga gattacaaag
    28921 ccagagcaac caaagcctga aaaacaatct gaagaaaagc agctcacctt agaagaagta
    28981 agggcggtac ttgcggaaaa aagccatgac ggatttacag ctgaagtaag agcacttcta
    29041 gaaaagtatg gtgcatcaaa actcagccag attgatccaa gcaagtatgc tgcacttctt
    29101 gccgatgcgg agggattgaa atgagtaaac atgcaattct ctctgcatct ggagcccata
    29161 ggtggttaaa ctgcacacca tcagcgaggt tggagctgga gtttgacgac aacagcggtg
    29221 aagctgcggc tgaaggcaca gctgcccatg cgttaagtga gcacaagctc cgcaaagcgc
    29281 ttaagatgag atcaaaaaag ccggtttctc catatgactc agatgagatg gacaattata
    29341 ctgatggtta cgtggagttt gtgcttgaag tgattgagca agccaagcag gcctgtagtg
    29401 atcccctgat cttgatagaa cagcgacttg atttttcaaa gtatgttcct gatggatttg
    29461 ggactgggga ttgcataata atcgctgata aggttcttaa cataattgat tttaagtatg
    29521 gtcagggagt cttagtcagt gcagaggaca atcctcaaat gaaactatat gctcttggtg
    29581 cactagatct ctttgatggc atttatgata tcgagatggt ttcaatgaca atctatcaac
    29641 cacgtcgtga aaatgttagt acatctacag tctcaaaaga gagcttatat caatgggctg
    29701 aagaagtatt gaagcctaaa gcagaattag cctttgctgg tgacggtatc tactgtcctg
    29761 gagaatggtg tcagttttgt agagcagcag ttaaatgtag ggcaagagca gaagcaaaaa
    29821 tgaaacttgc aagattcgag tttgctctac cgccactttt aactgatgaa gagattactg
    29881 atattctgtc ttccatcagc gaccttacaa gttgggctaa tgagattata gcctatgcaa
    29941 cagatgctgc agttaatcat ggcaagaagt ggccaggctt taaagtagta gaaggccgct
    30001 ccaaccgtaa atacaaagat gaagaagcag tcgcacaggc ggcaaagaat gcaggttacc
    30061 aagacatata taggcaaagc cttattacca ttaccgaaat ggaaaaatta atgggtaagt
    30121 caaaattcaa tgaagtcctt ggcgggctag ttatgaagcc accaggaaaa ccaacactag
    30181 taccaatttc tgataagcgt cctgaaatga acacatcatc agcaaaaaat gattttatgg
    30241 aggtataaaa actatgtcaa caacagcaaa aagaacaaat ccaacgaaag tagtaacagg
    30301 agttgttagg ctttcttacg ccaatgtgtg ggaaccaaaa tccatcaatg gaggtgctga
    30361 gaaatacagc gtaagcctga ttattccaaa gagtgatact aaaactttaa gtgctattaa
    30421 cgaagcagtg aatgctgcaa tagaagaagg tagaggtaag tttggtggca agattcctaa
    30481 taaagcggcg ttaaagcttc ctctgcgtga cggagatatt gatcgtccag atgatgaggc
    30541 ttatgcaaat agctatttta tcaatgccaa cagtattaca gctccacaaa ttgtagatag
    30601 aaatgtcaat ccaatccttg agcgttcaga agtatactcc ggtgtttatg caagagtaag
    30661 tatcaacttt tatgccttta actccaatgg gaataagggt atagcatgtg gtcttggaaa
    30721 tattcaaaaa atccgcgatg gtgagccttt gggtggaaga actaatgcag ctgatgactt
    30781 tgccactgac gtggacgatg actttttatc atgagaactc tcagtattga tatagaaaca
    30841 tatagtagtg tagaccttgc caaaagcggg gtctatcgtt atgctgaagc accggatttt
    30901 gagattatac tttttggata cagtgtggat gctgggccgg ttcaggtcgt cgacttggct
    30961 tctggtgagg ctatcccgca agaaatacaa agtgcaattc tagatagtaa ggttataaaa
    31021 tgggcattta atgcacagtt tgaaagagtt tgcttatcta aatactttgg tgtttggctt
    31081 gaacctgatt cttggcgctg caccatggtg tggtctgcct atcttggact tcctttatca
    31141 ctggaaggag cagcaataat aacaggagtt gataagaaaa agctgacaga aggaaaggag
    31201 cttatccgat acttctctgt accatgcaaa cctacaaggt caaatgatgg tcgtacacga
    31261 aacttacctg aacatgctcc agataaatgg gatagattta aggcatataa ccttcgagac
    31321 gttgaagttg aactttctat acaggaaaag cttcagaaat ttcctatgcc tgaagaggaa
    31381 tggaataact atattctaga tcagcaaatc aatgatcgag gtattcagtt ggatttggaa
    31441 ttggtaaaaa aagcgattca atgtgatgaa aaagtaagag aagagctaac aagcagacta
    31501 aaagacctaa ctgatcttga aaaccctaat tcagttgcac agatgaaaaa atggctatca
    31561 gaaaatggct tagaaacaga tagtcttgat aaggcatcag ttaaggagtt attaaaggat
    31621 gctccagatc atctgagtga agtgctggaa ttaagacaac tattagctaa gtccagtgtt
    31681 aaaaaatact ctgctatgga aaatgcagta tgtgctgatg gaagggctcg aggactactt
    31741 caattctatg gagccaaccg aaccggtaga ttcgctggaa ggttaattca agttcaaaat
    31801 ctacctcaga accatttgtt ggatctgaaa caggcacgtt gcttggttag aggtggtcat
    31861 tatgaagcct tggaattatt atatgactcc attccagggg ttttatcaga attaatcaga
    31921 actgcttttg ttccaaagaa aggatataag tttatcgttg ctgactttag tgcaatagag
    31981 gctagagtta ttgcttggat tgcaggcgag acatggagaa atgaagtgtt tgctactcat
    32041 ggcaagattt atgaagcatc tgcttcccag atgtttaaag tccccttaga agaagtcaca
    32101 aaaggtagtc ctctcagaca aaaaggaaaa attgcggagc tggccttagg ttatggtggt
    32161 tctgtaggtg cattaaaagc aatgggtgca cttgacatgg gtcttaccga agaagagtta
    32221 aaacctttgg tctatgcatg gcgaaatgca aatcctaata ttgtacgact ttggtgggat
    32281 gttgatcgtg ctgtcaaaga agctgtaaca gaaaggtgca gaaccgaaac tcatcgcacc
    32341 cgttttgagt atcgtagcgg gatgctatta atatggcttc cttctggcag acaacttact
    32401 tatgttaaac caagaatagg tattaacagc tttggcagtg aatcggtaac ttatgaaggc
    32461 attggtggca caaagaagtg ggagcgtatt gaaagctatg gtcctaagtt tgtagagaat
    32521 atcgtccagg ctctttcaag agatatcctt tgctattcca tgagaaagct tgatgagaaa
    32581 gattttgata ttgtaatgca tgtccacgat gaggtagttt tagaagttcc aatagaaata
    32641 tctgttcaag atatttgtgc tcttatggga catacacctc catgggctca gggacttttg
    32701 cttcgtgccg atgggtttga ttgtaatttt tataaaaaag attaatttta gggggttcga
    32761 agcctcctct ttttttgcat atagctgagg gcagatttta ttgctctact actatatgtg
    32821 ttggaggttc gatatgaaca aattaactat tttcaactac gaaggtaaca cagtaagaac
    32881 agtaatgaag gatggaagtc cttggtgggt tctaagagat gtgtgctcag tattagagat
    32941 tggaaacagt cgtgatgtta tggctcgctt ggacagtgat gaaaagggag tcgatattat
    33001 cgacacccct ggaggaaaac aggaagtatc tattatcaat gaaagtggtc tttatagcgt
    33061 gatattggtt tctcgaaagc cagaagctaa aaagtttaag cgctgggtaa cccatgaggt
    33121 gcttccttcc atcagaaggc atggccttta tgctacagat gagttacttg ctaatccaga
    33181 ctttttgatt caggcactac aggagcttaa agcggaaaga gctaaaaatg ctgagttaac
    33241 tactaccatt agcattcaag aacagcaaat tgcagaaatg aaacctaaag caagttatta
    33301 cgatgtggta cttaactgta aggatgcggt gtctatcaca acaatagcca aggactatgg
    33361 aaagtcaggg cgctggttta atgagtatct gcataattta ggtgttcaat tccgtcaagg
    33421 gaagatttgg cttctatatc aaaagtacgc tcaacatgga tatacgacga caaaaaccca
    33481 tacttacctt ggaaatgatg ggacgatgca ttcaaaggtt catacttact ggactcagaa
    33541 aggacgcttg ttcatttatg agcttcttaa ggatcatggc attttaccat tgattgaaca
    33601 agagtccgaa ttcgaggaga tgtaacttat ggatagatat aacgctgaag gctatccaga
    33661 tccaactgca gcagaagcct tggaaaatat catgcgtgaa gaaaaagcaa aaaactataa
    33721 accttgtgtc tttatctgct caccttttgc tggggatata gaagaaaatc tgaaaaaggc
    33781 tagaggatac ttaaagtttg cagtggagca aggaaccatc ccttttgctc ctcatctgct
    33841 atatcctcaa gtgctagatg atagtgatcc tgaacaaaga aaactaggat tattctttgg
    33901 tatggtctgg ctaagaaagt gtgaggagct gtgggtgttt ggtccctata tttcaaaagg
    33961 aatgcaagca gaaatagata aagcatcgaa gcatcgtatg gctattcggt attttactga
    34021 aaactgcgag gaggtgcaga agctatgaag atatcggttg gtaacagccg aatggacaaa
    34081 aagtggaaga acaaagacat aacatgggag gacttcatat cccgagttaa atctacaata
    34141 cgaaccacag aaacggtatc tgaatttcgg aaaatgagtc gtgcacagca ggactcaata
    34201 aaagatgtgg gtggattcgt aggaggagct ctacgtgaag gaaagcgtag aaatggctat
    34261 gtactctccc gctccctact tacattagat atggattatg gaaaaccagg gatttgggat
    34321 caaattgatg cattgcatga ttttaaatgt tgcatctact caactcacaa acacacacct
    34381 gatgcaccac gattaagact tataattcca cttaaaagag aagtgacaga ggatgaatac
    34441 ccagctctag gtcgtatggt tgcaaaggag atagggattg atttattcga tgacaccact
    34501 tatgaacctt cgagattaat gtattggccc tctacaccgt cagatggaga gtttgtcttt
    34561 aaagaaaaag atggagaact gttagaccca gatggctatc tttcaaaata tgaagactgg
    34621 cgggatactt caatgtggcc agtttcaagt cggcaatctg aggtagtgca aagaaaaata
    34681 actaaacaag cagatccctt aagtaaagaa ggagttatag gggcattttg cagagcctat
    34741 aacattgaag aagccatcga agaatttcta cctgatgtat atgaacctag taccatgaat
    34801 ggacgatttg attatattcc agcagattct tcagcaggct tggtaatcta tgatggtaaa
    34861 tttgcttata gccatcatgc taccgatcca gcatgtggaa tgttgctaaa cgcatttgat
    34921 ttggtccgag tgcataagtt tcgggaacta gatgaaaagg tgacagaaaa tacaccgcca
    34981 agtaaactac cttcatttag agccatgaca gatttggcat tagaggatga acgggtaaaa
    35041 gagcagtttg ctgaagaacg aaaggctcag gctgaaaaag agtttattga tgaagattgg
    35101 gaaaagcagc tggagattga taagacagga acggttaaaa atactcttag aaatttgatt
    35161 ttgatacttg aaaatgatcc caacctaaaa agcatagtgt ttaatcagct atctgatagt
    35221 ctcgaaatta aaggtgatgt tccttggccg catccatcga agttctggag agatgcagat
    35281 gatgcacagc taatcagcta cattgacacc cactacggaa ccttttctgc aagaaactat
    35341 gatgtagcgg tagcgaaggt cgctgacgat agatcttatc atccgattcg ggaatttatt
    35401 gaggctcttc ctgagtggga taaggtaccg cgagtagata ccctgctcat cgattatcta
    35461 ggtgcatcag ataacccata tgttcgggct gtcacaagaa aaactctatg tgcggctatc
    35521 tctcgtgtac tgactccagg catcaagttt gattccatgt tggttttaaa tgggccacag
    35581 ggagttggaa aaagcactct cattgccaag ttaggtggag actggttttc agatagcttg
    35641 aacttatcgg ataccaagga caagactgcg gcggaaaagc ttcagggtta ctggatttta
    35701 gaaattggtg aattggccgg acttaaaaaa gctgaagtcg aaacgcttcg aagtttcttg
    35761 tctcgccaaa atgacattta tagagctagt tttggcagaa gagctactcc ccacttaaga
    35821 caatgtatct tttttggtac caccaatgct gagaaaggct atttgaggga taccacagga
    35881 aaccgtcgct tctggccggt aaagactccg ggaaatggta caaaaaagtc ttggcagcta
    35941 aagcaggatg aaattctgca gatatgggct gaggctctta cctatgtgaa agctggagag
    36001 aaattgtacc ttgatgccag tcttgagaag cttgcaaaag aagaacagcg ggaagctatg
    36061 gaatcggatg agcgtgaagg tttggtacga gagtaccttg atatgctttt gcctgaggat
    36121 tgggacaata tggatttata tgaacgccga gcctatatca acgggactga gtttggtgaa
    36181 agccagaggg ttggtgtttg gaaacgtaaa tcggtatcca atatggaaat ctggtgtgag
    36241 tgctttggaa aggatcgagc caaccttcga agagtagatg gtaatgaaat atcagctatt
    36301 atggcgagta ttggaggctg gacaggtctc gtaaaaaaag aacgtatcca gctttatgga
    36361 ccacagtggg tttatgttcc aaaagaataa ttcagtttgg aacacatgga acaattattt
    36421 ctttggaaca gatttcacct gttccggtgg aacaaaaacg gtcttttggt acatctcatc
    36481 ggaacaggcg gcagcccctt gtaaagtagg ctacttaata gtctgtgttc cattgttcca
    36541 aaaattatta ttaataataa tcctaaagac aaaaagaaga aattacctgc agacgcgtat
    36601 atacgcgcgt atagagactt tttggattta gggaacatgg aggctatatg agagaaaaat
    36661 ggattgaaca acaactggta aaagcagtaa aagatatagg cggcattgca ctgaagattg
    36721 catcaccagg ttttgatgga atgccagaca gattgattct tttgccggat aaaaaaatag
    36781 cttttgtgga ggtaaaagct ccaggtaaaa ccttaaggcc tctacaggaa aagcgaaaaa
    36841 gacagttaga atcacttgga tttttggtat tctgcctgga tcacatagaa cagattggag
    36901 ggatacttca tgaaatacaa gcctcatgaa tatcaggttt atgccactga gtatatcctc
    36961 acccatccca tagcagcagt gcttttagat atgggattag gtaagagtgt cattacctta
    37021 actgccatct ttgatttaac actggatagt tttcttgttc gtaaggttct ggtcattgca
    37081 cctcttcgag tagccagaga tacatggcct gcagagcttg aaaagtggga tcacctaaaa
    37141 ggtcttaaat acaccgtatc agttggctct gaggtacaga ggaaaacagc ccttatgaaa
    37201 agagctcagg tatacatcat caatcgagaa aatgtcgaat ggctaatttc tagaagtgcc
    37261 ataccctttg attttgacat ggtagtaatc gatgagctgt catccttcaa atcccatcaa
    37321 gccaagagat ttaaaagttt aatgaaagtt aggccaaaga ttaaaaggat cgtagggctt
    37381 accggaacgc catcatccaa tggactgatg gatttatggg ctgagtatcg cctgctggat
    37441 atgggacagc gattgggcag gtttattggc agatatcgag aggacttttt cgtaccagat
    37501 aaacgcaatc agcaagtgat cttctcctac aaaccaaaac cgggagcaga agaagctatt
    37561 tataggctca tatctgacat cactattagc atgaaaggga cagactacct gaagttgccg
    37621 gagttggtta taaacgaagt agatgtaaag ctttctgaaa aagaaatgga aatccttgac
    37681 attatgaagc gtgatttaat tacaactgtt aaaggtgagg aaattactgc agccaatgca
    37741 gcagctcttt caggaaagct cctgcagatg gcaaacggag cagtctatga tgatcaaggt
    37801 gtagttattc atatacatga ccgtaagctg gatgcactgg aagacttaat cgaagctgct
    37861 aatggcaagc cagttctaat tgcttattgg tttaaacatg atttatcacg aatacaaaag
    37921 cgctttgaag ttgaggtatt atccactagc gattctatta aaaggtggaa tgatggagaa
    37981 atccccattg cagtcatcca tccagcatca gcaggccatg gactgaactt gcaagctgga
    38041 ggttcaactc ttgtatggtt tggcctgact tggagcttgg agctttacca gcaaaccaat
    38101 gcccgtcttt ggcgtcaagg acaaaatgaa acggtagtga tccatcacct gattgccaaa
    38161 ggcaccattg atgagcgtgt aatgaaagcc ctaaatgata aaaacaatac tcaatccgca
    38221 ctgatagatg cggtaaaagc aacactaaag gaggtctgat acgatgaaca ttgtctggca
    38281 gtatttagat aaaagagcag cggcaattaa tgccttaaaa gattacagca gcatgaagta
    38341 catcatagaa cataccgatg aggacattgc aaccctcaac gaagaaatga gctccccagc
    38401 ttctccagtt atcaatggca tgccatcgac ccatgatcca aaagcaggag agaaaaggct
    38461 cattgcctgc atcaatgaaa ttgatgtatt gaaagaacgt tatcgtcaag cactggaata
    38521 catggattgg tttcaacccg catgggatgc tttaacagaa gacgagcagt atgtgttaaa
    38581 ggagttttat ttggatgatg aacaaaagca gattgatgct gtgtataaca tctgtgatca
    38641 tttcaatata gaacgttctt cagcatacaa caaaaagaat cgagcgcttc aacatttagc
    38701 actactactc tatggaaagt aatgagtaat atcttggacg attttattag aaatccataa
    38761 tacaatggta ttgtgaaaaa ttgtagagag ccttcgtgga aataccacga gggctttttg
    38821 tatgtccaaa ggaggtgcga aatgccaaag aaaccaaaaa gaccatgttc ttatcctggt
    38881 tgtccggaac tgacagatgg acgcttttgt ccggagcata caaaaaaaga agcttctcgt
    38941 tatgaaaaat atcagagaga tcctgaaacg aggaagcgtt acggtcgtgc atggaaaaga
    39001 atacgtgacc gttacattac agcccatcca ctatgtgaag agtgtaaaag acaaggaaag
    39061 ctgacaccag caactgaagt gcaccatatc cttcccttgg cacgaggtgg gacacacgat
    39121 gaaagcaacc taatggctct ttgtactcct tgtcactcag ctatcacagc aagagatgga
    39181 gaccgttggc catcccggta gggggagtca aatctctaca gcttttaaac ggtcaacggg
    39241 cgtggggctt cgtgcaaaaa gtcgcagttt caaacggggt aataccccct taataagaaa
    39301 tgaggtgagt taatggccaa agatggtaca aatcgaggtg gtgcccgtat tggatctggt
    39361 cagaaaaaga aagcacttat agataaaatt gctgagggaa atcccggaaa aagaaaattg
    39421 gaagttattg aatttaaaaa taccgcagaa cttcaagggc aggagatgcc acagccaagg
    39481 gctatgcttt cagcagtaca aaaggatggt aaaaccttag tagccagcga aatttatgag
    39541 cttacgtgga aatggcttga ggagcgaggg tgtgcacatc tagttctacc acagctatta
    39601 gaaagatatg ccatgagtgc tgctaggtgg atacagtgtg aggaagcaat aagtgagttt
    39661 ggttttcttg ctaagcatcc aactactggc aacgctatcc aaagtccata cgtttccatg
    39721 agccataatt ttatgagtca aaccaacaga ctctggatgg aaatatatca gattgttcgt
    39781 gaaaactgtg cgacagagta ttccggtaca aatccacagg atgatgtgat ggaacgactg
    39841 ttgactgccc gtagaggtaa ataataagga gatgtgagat gagtaagaga tatttaacag
    39901 cagaaagtgt atgtgcagga catcctgata aactgtgcga tattattgct gataatattt
    39961 tggatgaatg ccttagaaga gataaagcgt cacgcgtagc gtgtgaggtt atggctacta
    40021 aagggaaaat tatcgtggcg ggtgagatct cctgcagcga gaaaattgat atcagaagca
    40081 ttgttaagaa tgtgctaaaa gaactaggtt ataacccttt gaaattttta atttatgtat
    40141 atgtacacaa tcagagttct gatattgcag ctggtgtgaa tactgcatta gaagcacgaa
    40201 atggtataaa cgaacaatat ggttccatcg gcgctggtga tcaaggtact atgtatggtt
    40261 atgccacaaa agaaaccaga gaaatgcttc cccttcccct tgtcttatct catcgaattg
    40321 taaagagact agatgaggca aggaaaggta aaattattaa aggtatcctt cccgatggaa
    40381 aagcacaggt gaccgttgag tatcatgatg atgttccagt gagagttaag accattgtag
    40441 tgtcagttca gcatgaaaag aataaaactc aggaagagtt aaaatcagat attctaaata
    40501 atgtgttatg gcagtgtttt gaggatttcc cttttgatga tgaaacagaa attcttatta
    40561 atccatctgg acagtttgta ttaggaggac ctgctgcaga tacaggttta actggaagaa
    40621 agatcatggt ggacacttat ggtggacttg catcacatgg tggcggcgca ctttgtggga
    40681 aagatccgac caaagttgac cgaagcggag cttacatggc taggtatatt gctaaacata
    40741 ttgtgtggtg tgatttggca gagaaatgtg aagtggctat ttcctatgcc attggcaagg
    40801 caaatcctgt agctttttct ataaatactt ttggaacagg aacagtttct gatgaagttt
    40861 tgacccttgc tgctcaagag gtttttaacc taaggcctgc agcaattatt gaaaagctta
    40921 gacttagaaa cattcactac tctgatacag cggtttatgg acattttaat agttgtcttt
    40981 tcccttggga ggatgttgat agatacaatg aattaaaagc agcggtggaa aaatatgtag
    41041 atagggaata tgtataagta gttatctgct agaaaaaaat gctccagact ttttatctag
    41101 aaagcacaga atcaacttta tctaaataac cataatttat cattaattta tctaaattta
    41161 tcattgacaa aaaatcaaaa ataacatatg attaaattag ataaattaaa ggaggtgtta
    41221 cgatgaatta tgaaaaagta attcttgaaa tgcttacacg gataaaggac ctggaagaaa
    41281 aagtagatat gttacaagaa taccagcaag agttgcaaaa caaagatgag ggtgaagatg
    41341 atataactag cggagaagaa aaaaaggaaa gtggaaggaa acgaacccgc agagagatta
    41401 tgacaattct agaagaaaag tatgggtata gtgttcgaaa aggaaatcgc tctgaaggaa
    41461 gtggtattgt agcaagtaag aatgggaaat catacaatat caaggtttca tatagtcgtt
    41521 catacactga tagcgatgag gtaatttgtt ctggatggca tactgtattt gataaagaaa
    41581 tagataatcc agatttccca tatttcatct ttgtagttga aggcaaagag agtgaattcc
    41641 attactttat tttcaaacgt gaggacctta ttaatgagtt tgacgataag gcttatgatg
    41701 ccaacaaaaa actccacttc tatttccgag taacaaagga tggtaaacca cttgaagtca
    41761 gagagacgga aaaagatatg actgcctatt ataacaactg ggatatattc aaatagtaaa
    41821 agcaaaactt aaagattcat caatcaataa cacgcttcta ttaaatgagg cgtgttttta
    41881 ttttgggagg ttagcatgaa tatagataag atttctatca agaagttgat tcctgcagat
    41941 tataatccta gaaaggattt aaggccaggt gatccagagt atgaaaaact aaaaaggtca
    42001 tttgaggagt ttgggtatgt tgaaccaatt atttggaata aaactacagg tagagtggtt
    42061 ggtggccatc agagactgaa agttctactg agcatgggta tggatgtagt ggattgtgta
    42121 gttgtagaga tggatgagga aaaggagaag gctcttaata ttgcattgaa taaaataagc
    42181 ggagaatggg ataaggaaaa actggcactt cttattacag atttaaacgc tgcagacttt
    42241 gatgtatctc tgactggatt tgatccagga gaactggatg atcttttcaa ggattattta
    42301 aaagacaaaa taaaagaaga cgattttgat atagatagcg agctgaataa gcccgctatt
    42361 tcgcgtttag gggatgtttg gatacttggt aggcacagaa tggtatgtgg agatagtaca
    42421 aataaagaga ccttcaatac attaatggat ggaaaagttg ctaatctggt ggtaactgat
    42481 cccccataca atgttaacta tgaaggtact gcagggaaaa tcaagaacga taatatggca
    42541 aatgatgctt tttataagtt tctattagat gcctttataa atattgaaac agcactggca
    42601 acagatggaa gcatttatgt attccatgcc gatacagagg gacttaattt tagaaaggca
    42661 tttgtcgatg caggttttta tctttctggt acttgtattt ggaagaagca atccttggtt
    42721 cttggaagat ctccttatca gtggcagcat gaaccagtac tctttggttg gaaaaagaag
    42781 ggtaagcatc tctggtattc agaccgcaag caatcaacta tctgggaatt tgataaacct
    42841 aaaaagaatg cagaccaccc aacgatgaag cctgtttccc taattgctta tccaattatg
    42901 aactcaagcc taacaaatag tatagtgtta gacccttttg gaggttctgg ctcaacccta
    42961 attgcttgtg agcagactga tagaatttgc tatacaatcg agctggatga aaaatactgt
    43021 gatgttattg tgaagcgtta cattgagcag gtaggaacag ataaagatgt ctatgtgatt
    43081 cgtcaaaaag agaagattcc attcagtggg gtagctacat cttctgacga aatatgttga
    43141 tagaagaagt tatacattca ttcaaagaaa tgacttgcta ttgtgtaact ttagagtgat
    43201 atatgttact accaaataag aaaggtggta tgtagcatga aaattgaatt taatcgcact
    43261 ggcagtgaaa ggaaggccct agttaccgcg attggggaaa tacttggtga aaagcctaaa
    43321 tacaaaggcg caccaacatt tgtttatcaa ataggcagat ttgaagtgaa taaggaaggt
    43381 gctcttattt ttgatgaggg tgttgttggc gaaaaagcga ccatactcct agatgagctt
    43441 gaaaggcaag gattttcata tgtgaaacag gattgtttgc tagcagagca tttagaagat
    43501 aaagacttgc tagttattga aattcctaag agagatttca atgataaagc ctatagcaac
    43561 ttggaaaaga ttctggaaag caaaggagct ctcattaaga aggcccttgg agtagaggaa
    43621 ctacctgttg aacaaacaga ggagtcgctc cggtttcctt ggttttacct tgacggagat
    43681 gctgagaagg ttaaagcata tacacatttt attacagccc tttgtgatat ggcaaagaag
    43741 caaaagagaa tcactgccac agctaaagaa gcagacaacg aaaaatatgc ctttcgatgc
    43801 ttcttattaa gacttggttt tattggagaa gaatacaaga catcaagaag aatacttctt
    43861 tccaaactaa gcggcagttc tgcattcaag agtggagttc ctaagcgtga ggaggtgaat
    43921 gtaaattgaa aaccgttcac ccaaaaataa taaagcaact aaggagtgtc tatacacctg
    43981 gaacaagagt ggttttagta aagatgaatg acccctatac caaactagaa cctggggcaa
    44041 gaggtactgt cattggtgta gatgatattg gaaccattca tgtcaattgg gactctggta
    44101 gcagcttagg agttgcatat ggcgaggatt cctgcagaaa aatcgaggag gaaaagaatg
    44161 aatgacatta taaaaagcca gatttttgcc attagagata caggcgaaac taatatgttt
    44221 gatcttccct tattgaccag cattgcttta agagaaggat atatagagct tatagattat
    44281 cttgaaaaga ataaggaagc gtatgtccat tttattctga cagggaaagt agaaacagaa
    44341 taataaatag actattaaat cttagggaac tcttcggggt tccttttttc gtagccataa
    44401 ggaggtggcg gccatacgta aactaaaaaa atataagccg accatcttta aggcagatgg
    44461 ttcggtatat gataaggacg ctgcagacat tgcggtgtct tttattaatt gcttaaaaca
    44521 tacgaaggga gaatggtatg ggcaaccatt tgaacttata gactggcagg aacagattat
    44581 ccgcgatgtg tttgggatta taaagcctaa tggttaccgt caatttaata cggcatatat
    44641 cgaaatcgct aaaaagcaag ggaaatctga acttgcagca gcggttgcgt tactgcttac
    44701 ctgtggtgat tttgagcatg gcggtgaagt atacggatgt gcatctgaca gacagcaagc
    44761 ttccattgtt tttgatgtag cagtggatat ggtagaacaa tgtccagctc tgaaagcaag
    44821 aattaaaccg gtactatcgc aaaaacgact tgtttataaa ccgctaggta gtttctatca
    44881 ggttttatct gcagaagcct ataccaaaca tggactaaat gtgcatggtg tggtatttga
    44941 cgaactacat gctcagccca atagacagct tttcgatgtt atgacccatg gttcaggtga
    45001 tgcaagaaag cagccgttat atttcctaat taccactgcc ggcaatgaca cacattcaat
    45061 ttgttacgag gtgcatcaaa aagctaaaga catcttggaa gggcgaaagg ttgaccctac
    45121 attttatcca gtagtttacg gtgctgatga agatgatgac tggactgatc caaaagtgtg
    45181 ggcgaaagcc aacccctcaa tgggcattac cgttgatata gaaaagattc aaattgcttg
    45241 tgaaagtgca aagcaaaatc cagcagaaga gaacctgttt aggcagttgc gtttgaatca
    45301 atgggtaaag cagtcgatac gctggatgcc aatggaaaag tgggataaat gtgcttttgc
    45361 tataaacaag gaaagtcttg aaggacgtgt gtgctatggt ggtttggatt tatcgtctac
    45421 cactgatatc acagcatttg ttcttgtctt tccacctgag tatgatgatg acaaatatat
    45481 tattcttcct tacttttgga tacctgaaga taacattgaa cagagagtga gacgagacca
    45541 tgtgccatat gatatctggg agcagcaagg ctttttatac accacggaag gaaatgtggt
    45601 gcattatggc tacattgaaa actttataga agagctgggc ttaaaatata acattcgaga
    45661 aattgccttt gaccgttggg gagcagttca aatgacacaa aaccttgaaa atcttggatt
    45721 tacggtagta ccttttggtc aaggctttaa agatatgagc cctccaacta aagagctaat
    45781 gaagttaaca ttagaggaaa aactagcaca tggtggacat cctgtactgc gatggatgat
    45841 ggataatatt tttatccgta ctgatcctgc tggaaatatt aaaccagata aagaaaaatc
    45901 aactgaaaga attgatggag ctgtggctac cattatggct cttgaccgag caatccgcaa
    45961 aggtggatca ggaaattctg tttatgacgg tcgagggctt cttattttgt agcaaaggag
    46021 agtgatgcag atgggacttt tatcaaatat tttcaaagcc cgtgataaac ctcagaaccg
    46081 gaccgtaggt agtaattata gcttcttttt tggaggaact actagtggta aaccagtaaa
    46141 tgagcataca gctatgcaaa tgacagcggt ttattcttgt gtaagaatac tggcagaggc
    46201 tgtggcagga cttccgcttc acttatataa atacacagaa agtggaggta aggagaaagc
    46261 aatatcacat cccttatatt ttttattaca tgatgaacca aatccagaga tgagttcttt
    46321 cgttttccga gaaacgttaa tgactcatct tttattatgg ggtaatgcct atgcacaaat
    46381 aattcgaaat ggcaaaggag aagtcatagc actataccca ttaatgccta atcgaatgtc
    46441 tgtggatagg gattccagcg gttctttata ttattcctac accagatatt ccgatgaagc
    46501 acctacgatg aatggaatga cagtcacact aaggccaagc gatgtactcc atattcctgg
    46561 cttaggattt gatggactag tagggtattc tccaattgca atggccaaga atgccattgg
    46621 tatggccata gcctgtgagg aatatggagc taagtttttc gcaaatggag cagctccggg
    46681 aggtgtactt gagcatcctg gaacgattaa agacccacaa aaagtgcgag atagctggaa
    46741 tgcggcttat caaggaagca gcaactccca tcgtgtggca gtgcttgagg aagggatgaa
    46801 gtatcagtct attggtatct caccagaaca agctcagttt ttagagacaa gaaagtttca
    46861 gattaatgaa atcgctcgga ttttccgcgt acctccacat atggttgggg acttggaaaa
    46921 atcgagcttt tctaatattg agcaacagtc actggagttt gtgaaataca ctttggaccc
    46981 ttgggtgatc cgttgggagc aggccatcag ccgatcactt ttaagaccag atgaaaagaa
    47041 gctctatttt gccaagttta atgtggatgg actgcttcga ggtgattatg tctctcggat
    47101 gaacggttat gcaaccgcga gacagaatgg ttggatgagt gccaatgata ttagggagct
    47161 tgagaacctt gatcgaatcc cacctgagct tggcggggac ttatatctaa tcaatggcaa
    47221 tatgaccaag cttgcggacg caggcatatt cgcaaataaa gaaggaatgg agggaaaacc
    47281 tgaatgaaga aattttggaa ttgggttcgt gatgcagata cacagacacg aaccctatat
    47341 ctaaatggcg ctattgcaga ggagagttgg tttgaagatg atgtcactcc agctgctttt
    47401 aaagaagaac taatgagtgg tgaaggtgac atagtagttt ggatcaattc tcctggtgga
    47461 gattgcatag cagcatctca gatatacaac atgctaatgg attataaagg aaacgtcact
    47521 gtaaagattg acggtattgc tgcatcagct gcttctgtca tcgctatggc agggacggaa
    47581 gtcttaatgt ctcctacctc actgatgatg atccataatc ctttcaccat agccattggc
    47641 gatagtgagg agatgcaaaa agcaatccaa atgcttgatg aagtgaaaga gagcatcatc
    47701 aatgcatatg agcttaaaac cggcttatct agaacaaggt tgtcgcacct gatggatgct
    47761 gaaacttggc taaatgccaa taaggcagtt gagctaggtt ttgcagatga cattatgttt
    47821 aaaccaggag agagttcact acaagacagc tttgtattca gcagaagagc agtgaccaat
    47881 tcactaatga ataaacttca aaaaccagtt gtaaaacagt cagtagagcc gctttatgag
    47941 cggcttaatt tattgaaata ttaggaggaa tgaaaatgag taaaattctt gaactgcgtg
    48001 aaaaacgcgc aaaagcatgg gaagcagcta aagcatttct tgattcaaag cgtggtagtg
    48061 atggacttgt gtccgcagag gatgccgcaa cctacgacaa aatggaagca gatattatta
    48121 atctgggtaa ggaaatcgca agattggagc gccaagaagc tcttgaagca gagcttaata
    48181 agcctgtaaa cacacctctt accgaaaagc cagctattcc ggggatggat acaaagaccg
    48241 gaagagctag tgatgagtac aggaaggcat tttggaatgt aatgcgtagt aaaaaccctc
    48301 gtcatgatgc actaaatgct ttatctgttg gtactgattc tgagggagga tatcttgttc
    48361 ctgatgaatt tgagcgtacc ctggtccaaa ctcttgagga agagaatgta tttcgtaaac
    48421 ttgcaaaaat tattcagaca tcaagtggtg atcgtaaaat cccagttgtg gtgaccaaag
    48481 gaactgcagc ttggcttgac gaaggtgagg agtttgatga gagtgattct gtattcggtc
    48541 agacatcaat aggtgcctat aagctgggta caatgattaa ggtttctgat gaacttctta
    48601 atgacagcgt atttgattta gagaaatata tttccactga atttgctcga agaatcggtg
    48661 caaaggaaga agaagcattt cttgttggtg acggagatgg aaaacctaca ggaattttca
    48721 atgtaactgg tggtgcacaa cttggagtgt cagcaggatc tgcaacagct attactgcag
    48781 atgaaattat agaccttgtg tactcattaa aagcaccata tagaaagaat gcggtattcc
    48841 tgatgaatga tgcaactgta aaagcaattc gtaagctaaa agatggtcaa ggccaatatc
    48901 tatggcagcc ttctttaaca gcaggtactc cagatacgtt gctgaatcgt ccggtttaca
    48961 cttctgctta tgctcctatt attgaagccg gagcaaagac gattgctttc ggtgatttcg
    49021 gatactattg gattgctgac agacaggggc gttctttcaa acgtttaaac gagctttttg
    49081 caactactgg gcaggttggt ttccttgcaa gccagcgtgt agatggaaag ctcattctac
    49141 ctgaagccat caaagttctt cagcagaagg cttaatggga ggtgcaaatt atgagctata
    49201 gcgcaaagaa ctacaccgaa caaggtggag aaaaaaccgt tattggtgga gagcttgtca
    49261 ttgaagaggg agccaaagta actgggctcc ctgttcttga aaatcaaccg gcaagcactg
    49321 cggaaactgt agaagctcta gtgacggact ttaatgcctt gctcagtaag ctgaaaactg
    49381 caggaatcat gaatggagat acaccttaga aaggatagtg atggtgatga cacttttaga
    49441 aaaagttaaa gcaaatctaa ttcttgagca cgatcgcgat gatgaacttc ttcaaatgta
    49501 catcaccacc gctatcgcat atgccgagag ttaccagcat gtaccggaag gtcattataa
    49561 tgagaacaca atgccgccaa ctaccgagca ggccgtcatt atgttgtcat ctcacttcta
    49621 tgaaagtagg gatggtagca ctggcggctt ttttgctgat aacgtgcagg caggccagca
    49681 ggtttggaac actgtaaatt tactgctcag gcttgaccgg gattggaagg tgtagagtat
    49741 gagttttgga aaaatgaata cctttatcga tctcatttct gttgaaagaa cgaaagacag
    49801 tgaaggcttc ggtaaatcta aggacaccat cctcgcttcc gttcgtgctt ataaggaaga
    49861 tcgtcatgga aatgaaaagt gggctaaccg agcgacattt tctgaagcaa ctgcgctgtt
    49921 tcgttttcgt aagatacctg atgttgaggt atctaccaat atggtgattg tgtgtaatga
    49981 tggccgctat gagattacaa atgttgaaga tgtaaaaggt cgaggcatgt acattgaagc
    50041 catggcaaaa aaggtggtgg ggtcaagtgg ctaaggtaca agtgaaaatg cctgaggatt
    50101 ttcttttaaa gctttcaaag cttggcgata agacggatga aatcattcca aaggtacttg
    50161 agtcaggcgg ggaaatcgtt ttggaaaagg ttagatctaa tttgcaagct atagttggta
    50221 gtggaacaaa agaaaaaagt cggtctacag gtgagcttat tagttcgttg ggtctctctc
    50281 ctgctaaagt ggaccgaaat ggcaatttca acgtgaagat aggttttaag gagccacgaa
    50341 gaagcggcga aagtaacgct aagattgcca atatcatcga atacggaaaa tcgggtcagc
    50401 caccaaaacc atttttaaag cctgcaagaa gggcttcaag aaaagcatgt attgacgcta
    50461 tgaagaagag gtttgagcag gaggtcgaaa acttatgagt atattaaatg agcttaatct
    50521 catagcagat ttgtgcggta tcccagtaga aacaggaaga ttttctggtg ttcctcctga
    50581 tacttatctt gtgattacac ctcttattga tttgtttgag gttcatgcgg ataacacacc
    50641 gggatatgaa gtacaggaag ccagactttc cttatttgtt aaaggtagtt atacagctat
    50701 aaaaaatgca attgtccgca ctcttctagg tgcggatttt acgataacgg atcgtcgata
    50761 catcggacat gaggatgata ccgattatca tcattatgcc atagatgtgg ctaaaccata
    50821 tgaatttcaa ttggaaaagg aggaataaaa catggctaca atcggtcttg atagacttta
    50881 ttatgcaaaa atcaccgaag acataaacgg tgatgaaacc tatgatacac cgatgccgct
    50941 ggcaaaagca atcagtgcag aactttccgt tgagcttgct gaggccactc tttatgccga
    51001 tgatggtgcc gctgaaattg tcaaagaatt taaaagtggt accctaactc tcggtattga
    51061 tgatataggg gtagcagctg caggagattt aacaggagca accattgatg acaaccatgt
    51121 actcatttca accagtgagg atggaggggc tcctgttgca attggcttta gagcacaaaa
    51181 agcaaacggt aaataccgat acttttggct gtatcgtgtg aagtttggaa ttcctgcaac
    51241 aaacttggcg accaaaggcg atagtatcac tttttcaacc ccaactatcg aaggaacagt
    51301 gcttcgaaga aataagctag atggtcaggg taagcatcca tggaaggcgg aagttaacga
    51361 gggtgatgaa ggggtaactt caacggttat taatagctgg ttcaacgaag tgtatgagcc
    51421 cacattcgca gcttctagtg gaattggaga gtaaggggga attgaaatgg ataacgaaag
    51481 aagtgcaaag atcaatattg gcgggcagga gtatgagctt attcttacca ctaaagctac
    51541 aaaagaaatt gctgggagat atggcggtct tgaaaatttg ggtgagaaat tactgaagtc
    51601 tgagaacttc gaaatggcac tggatgaaat agtctggctg ataactttaa tggccaatca
    51661 gagcctactc attcacaacc ttcgaaatcc agataacaaa aagccgctgc ttactcaaga
    51721 agaagttgaa cttctcacat ctcctttgga actagctact tataagaatg ctttgacaga
    51781 agcaatgttt aagggaacca agagaaatgt agaatctgaa gatgactcaa aaaacgttta
    51841 aaccgggtaa acgagaatga actctttacc cggcttttat attacgggac agttcattta
    51901 aatcgtaccg aagaggaaac atggcttaca cctattggct tgcttatgga tttatgggag
    51961 tgccataagc agtttcttgg aatgtccaaa ccaaaacgga agatttacat cgatgacatt
    52021 attccttatg gtatttgatc tttcaaaaga aaggaggcgg taatcttggc agataatttt
    52081 ggtttaaaaa tcggagtgga aggcgaaaag gaattcaaaa atgcacttcg tgacattaac
    52141 caagcattta aagtattagg tagtgagatg gctctagtca gttcccagtt tgataaaaac
    52201 gataaatcca tccaagcctt aactgcaaga aatgaagtat tagaaaaatc cattgatgca
    52261 caaaaagaaa aaatagagac actacgtgct gccctagata acgcctctac ttcttttgga
    52321 gaaaacgatc gtagaaccca aaactggcag attcaactaa ataaagcatt ggctgaactt
    52381 aatggcatgg agcgggaact tgaaaataac cataaagcgc tacgagacca tgccgacgcc
    52441 acagatgata gtgctgataa catggaagat gccgctgatg cagccaatga acttgcagat
    52501 aatgttgatg atgttggcga tgaaatggat gatgctagca agaaaacctc tgtttttgga
    52561 gatgtactta aagcaaattt gttatcagaa gccataattg gtggggtaaa agctttaggc
    52621 tcagccattg cgggaattgg aaaagccttt ataggcgcta tgaaagatgg cgttgagtac
    52681 aacgctcaaa tggaaaacta cacagcttcc tttaccacca tgcttggtga tgaggctaaa
    52741 gctcaaaagc tggttaatga tctgaaaaaa gaagcagcag ctacaccatt tggaatgcag
    52801 gatcttgccc aatcagctca aacccttatg agctttggta tgtctgcaga agaagctcaa
    52861 aaacgcatga agcagctggg tgatatatct cagggagatg ccgagaagtt caaaagcctg
    52921 acactggcat ttgcacaaat gtcctctact ggtaagttaa ccggacagga cttgatgcaa
    52981 atgattaatg caggatttaa cccactagag gagatctcgc gtaagacagg aaaatccatt
    53041 ggtgaactta aggatgaaat gtccaaaggg gcaatatctg cagatatggt cgcagaagcg
    53101 tttgcctcag ctacatcgga aggtgggcgt ttctatgggt caatggaagc tcagtcaaaa
    53161 accttttctg ggcagatggc aactctcgaa gatggtgttg cttcattgaa gggccaactt
    53221 gctgaaggct taacaactat gctttccggt accgttcttc ccatggttaa tggttgggtc
    53281 gatgaattgt ctggagcatt tgaaaaagac ggtgttcaag gcttaattga tgcttttgga
    53341 ggaatcttag aggaagcagt tcagtttata tctgagcagt tgccaattgt agtagatatt
    53401 gcttcgcaga ttattatttc tctggttcaa gggcttacca ctgcattacc gcaaataaca
    53461 gaagctgcag tcatgctact gatgacatta gtcaatggaa tcattgaaac acttccagcg
    53521 ctaattacag caggtattca aatgattgga accataataa gtggaattgc agaagctctg
    53581 ccacagttaa ttcctgctgc agtttcagcg gtggtacaaa tcgtacaggg gcttttggat
    53641 aaccttccta tggttttaga agcagctctg caacttgtac ttggattaac tcaaggtatt
    53701 ttagatgccc ttccagtgct gatcgcggct ttgccagcca ttattactgg catagtaaat
    53761 tttgttatag gcgcaatacc tcaaataatc gaggctggta ttcaacttct gacatcttta
    53821 attggtgctt tgccagaaat cattaccgcc attgtagaag caattcctca gattgttgac
    53881 gggttaatca cagcgatttt gggctcaatc ccactgctca ttgatgctgg ggttcaactg
    53941 ctagtggcac tgattcaaaa tctaccacta attattacaa cagttattac tgctatacca
    54001 aagattgtat cctcccttgt gaatgctatt attggcagca taccacagat cattcaagca
    54061 ggaatcatgc tgttggtttc gctgattaag aaccttccag ccattatagt agaggttgtt
    54121 aaagcagtgc cacaaattat tgcggcactg gttaaaggtt ttacaggatc tatttggcaa
    54181 attgcccagg ttgggaccaa tttaatcaaa ggtttgtggc agggtatttc agatgctgga
    54241 gcctggcttt gggataaaat ctcaggcttt tttggtggtg tagtagataa aataaaaaat
    54301 ttctttggaa ttcgatcgcc atctacattg tttgctggaa ttggcgaaaa catgggagag
    54361 ggtattggtg tcggttttga aaaagccatg aatagtgtta gccaggatat gcaaaacgct
    54421 atccctactg attttgacat taaccctaat ctgaatatga atggagccgg tattagtagc
    54481 ggatatggaa catttggagg gtcattaatt acgatacagc agatgattgt tcgaagtgaa
    54541 gacgatattc ggaagatttc acaggagtta tacaatttga tgcagacagg atctcgagca
    54601 caaggaagat ttataactgc gtaaaggagg gcgatgtatg ggctttattt ataacggtat
    54661 taactcaaag aatatgaaga taaaagcaag gctcacaagt tggcaggcat cgcccgcatt
    54721 aagaaacgcg tatgaggttg ttccggggaa ggcaggggtt gctgactttg gttgtgatat
    54781 ctcagagcgg atcattacga ttagctgtag catatatcct caaaagagct ttgcagattt
    54841 agtgagcgtg ttagatgatc ttgcagagtg gctcaaccct atgaacggcc ttaagcagct
    54901 tatattagag gatattccga atcgattctt ttttgctcgc ctcactgaac aagtagaatg
    54961 cgagagatta cttagaactg caggtgcctt tgagttgaag tttatctgtc ctgatcctca
    55021 cgcctatgcg ctgacagatg aacaattcac gatttcaagt gttggaactc atgagatcca
    55081 aagaataacc gggaatacag attcagaacc gatatatcaa cttaagggat caataagtgg
    55141 atctacatcc acatatattt ctattaccac gaatggagag gaacttcgag tagttggagc
    55201 tttgacggca gatgaagtgc ttgtaattga tagcggatta gtgacagcaa aaataaccga
    55261 tgccaacgga aatacacttc gaaatgggct tccagtcctg gatgagttaa acttccctgt
    55321 acttcacaaa ggtgaaaatg aaataacagt atcagctgtg ggagcaacat tttcggagct
    55381 gaagattctg gccaaaagca gatggaggtg atctggtggc aattaaatca atcttaacat
    55441 cacagacgga ttttacgggt gagtttcctg taagcgagaa gacagttgct ctgtggcgat
    55501 ttaatgaagc cgcaccagat agcaacaata tgcttacaga tgactccggt cataacagaa
    55561 acttctttgt atccggatgg tcaggcacat cagccaactt gttatccggt agattaggaa
    55621 gatatttcag gcagaatatc attaatccaa caagtgaaaa gactcattta gtggctacca
    55681 atgacggtag cttttttagt gatttaggtg aaaagattgt tgttggcgga tggataaatc
    55741 caactactta ttctgtaggg cagacgtata tcccgatttt taatactaga caagggcctg
    55801 gtcagccaat tttttatgtt tctttatttc agggaaggtt gcgattaatg ctgtataatg
    55861 cctcaggctc cttgatatat gatcagaccg aaacgcctac tattacccta aagaataacg
    55921 ggtggtactt tatcgcttct gttatcgagg taaacaacaa aagggtgcag aacttacttt
    55981 gtgaccgaag tgatggcgct gtttggcaat ctccagttcg aacattcact ggagacttaa
    56041 atcaatcttg tgttgcggat attgtgatgg ggatgcatgc aaatacctat tattacgcag
    56101 gaggatttga tgactggttt tatgaaaaag attctttact taccatggag gatcttattt
    56161 cctatttcaa atcttcaatt ttggcaaacg gtggtgacag tgctgctgat gtagatgctc
    56221 ttgcggaccc tggctctgtc atgttgaaag caacaaacgg agtctatgca caaagtggtc
    56281 agctttttac gatagcagct gcctgtagtc ttgcaggaac gggcagggta tcagttacaa
    56341 gtgaatacac cgcaggtata acagccataa gcctagtgga aacttctaca tcagacgatc
    56401 tgtctagctg gactgaatgg caggccgttg gagcaagtgg ggaactgcag tcgcctaaca
    56461 gagaatatat ccgttatcga attacattat ctactcaaga caccagtaga actcctaaac
    56521 ttcttgaaat acaactacat gatataccaa aacctcctta tgagagactt ggatttgcca
    56581 gaccagttgt gttggatact aacggggctt gggaagcggt gttagaaaat gcctttgata
    56641 ttgtggtaac aagtgaggtg aatggagctg atattttgga gtttaaactg ccatttcatg
    56701 attccaagcg cgagacctta gacaatgaaa aacaggtgca gattgtcaat gatgtttatc
    56761 gtattcgaac cataacagat gagaaaagct cagatggaag agttgtaact caggtatatg
    56821 cggaagctgc attttatgat ctttccttta gcgctgaaaa agaacccatg gaatttgtcg
    56881 cagagacacc agaagtacct atgcgctatg ccttacttgg tactggctgg tcattaggaa
    56941 atgtcactgt cagtacaaaa cgaacatggc aatcaacaga gaagaatgct ttatctattc
    57001 ttcgagccat acagaatatt catggtggtg acttgatttt cgatagtgcc aatcgtctgg
    57061 tacacctttt gacatttgga ggtactgata gtggggcatt attttgctat agaaagaaca
    57121 tgaaaagtat acagcgcgta gtggatacta gaagtctaat cactcgcctt tatgcttatg
    57181 gaaaagatgg tataacattt gcttctatca atggtaataa agagtatgtt gaggattata
    57241 gctattcatc agaagttaga gttggaacgc tggatgcttc atcaatcagc aacccttatc
    57301 agctgcttga gttcgctaat atgcgcttag ctcagtatgc gaagccacga atctcatatg
    57361 ttctctctgc aatggactta tcagtgttga caggatatga gcatgaggca tggaaactag
    57421 gtgatatagt aactgtggat gatagagatt tgaagttatc tgtcaaaact cgtgtagttc
    57481 gcagacaata taatctacaa gaaccatgga aaacagtgct ggagctatca acaactttaa
    57541 gagaattagg agattcctct gctggatggg ataaagcagc cgatatttta tcttccactg
    57601 atgttcttga ccgtcaggaa ttaaaagact tagtgccttt taatcatcta cgaaattcac
    57661 gagctgatga tggtctaacc tattggttaa gttcaggttt tacagttgac cctaataatg
    57721 gtgtatcagg aacagcttct tttaaagctg agggcgtttt agggatgacg aaaagtctat
    57781 cccagaccgt ttatcctgcc agtaggaaga gttacacttt ctcagcgcag attgcatcgg
    57841 aaaatcttca gaaagggcca aacgggcaag ttgggattga agttgtcatt gaatatgagg
    57901 atgggtcgac agaaaccagg tttattgatc tcttttagga aggagccagt gctatggctt
    57961 atttttcaca gacagcacat gctattacac cgagaggtat tggaaagata aagtcgctta
    58021 ccatccgtct tttcattact gattgttctg gtgaggtgtt ctttacagat atgcttttgc
    58081 aaggtggttc tgttgctacg ggctggattg gtcatgtgtc agaaattaag tggacgttgg
    58141 atgggtaggt ggtgcagatg tcagtaacat ttaccaggtt tgctgagacg attcattgta
    58201 aagaggataa acgagtagta agcgtaacag tgaaactact tcttggagat tgcacaggta
    58261 ctgtttattt cactgatatt caggctcagg aaggtgatcg tctaactggt tatacaatta
    58321 atacagaaac gatgcttcaa aaattccggg agggcggtgt tattgttccc gctcgttttt
    58381 ataacggagt ggttcgtagc ggagagacgg ttattctctt caatctgggt tcaacttccg
    58441 ctggccttga ctgtcatatt tatcccaacc aaaatatggc tgcaggcagt atccaattgt
    58501 ctcaaggagc aggggcgcac aaggtgatat ttaatgaggc agttagtcca ggtgatacct
    58561 tttcgctact agcatcaact aggcagtgtt taaagaacgg aaacccaact gataaggaag
    58621 gtttttttca atatacagca tccggtgata gcaaacatgt gataaagcta gaagacagaa
    58681 aatcagcccg ggtattattt gagtttcaag aaatgcagga aggaagtgag cgcctttgat
    58741 tgactattta aaaggtaagc gttgtatggt ctggagtttc atggggaata cccgcatgta
    58801 tcaagcatta cgagactatg gtgatcgaat tgatacggtg ggtattttta cttttgaagt
    58861 ggatatcacc gggaccataa cagaaacagg aacaagcata tccagtatgc ttacttacat
    58921 taaccgctgg cctcatatca aatggctgct tacaatcatg aaccatggta cggcttctat
    58981 ttttacagcc cttagaaata acaccaatgg tgcaaaggat aaatttctta ctgagattat
    59041 tcgcatcatg aataagtatc cttggtgtgc tggagtggat atagacttgg aacgtggagg
    59101 tggctatgaa aacaaggatg ctgctaatgc tctatttcga gacatatata actctgttaa
    59161 agcctatgat tcttcgaaac tcgtaaatat ttgtctaccg ggcatgactg gagtacaagg
    59221 ctcggttggt ggcgaaaact ggtgtgttta tgaagacttg aatgcctatt gtgatacagc
    59281 agcgattatg agttatggca tggcgtgggc aggctctgca ccgggttcgg tatctcccag
    59341 ggattggttg gaaggcattt atgattatgc agtccgggtt atgactcctg aaaaaatatt
    59401 tttaggactg cctgcatatg gttggaactg gagaatccac gatacaccag aaaatctggg
    59461 catcacttat cgtgggattt caaacacata ttatgcggca cagctttgga tgacaggggg
    59521 atataacttt acggatgatg gaccaccaca gcccatgatt cctatcatag cgtattggga
    59581 tgattatgat aaggtgcctt gggccttgcc acatgtgtat gactacatgg aaggctggga
    59641 tgcagtttca agaacctatc ctttactggg tgaaacttat aaccgtcgca ggtatttgac
    59701 tgcctatagc aagcagcaaa aaactgaatt tggcacgata tatgtagatc gtagtggcgg
    59761 aactcctgat agctatactg gtaatgtatc ggtttcttct caaatgataa cacttggtga
    59821 agaaggtgaa gccgaatacg aatttgaaat ttcatctgct ggtatttatg atgtagccat
    59881 tcgcatctca tttcccttct gggataagaa tagcattcat gtttcacttg acgggacaag
    59941 taaggtattt agcgagaata ggctgtggtg gccatattgg cgaacaactt gttggctttc
    60001 cttggtttct ggtgtatttc tttcagctgg tacccatact gtcagcgtta gtacctcagt
    60061 accaggtgtg cagttctatg gatttcgagt atgttcgagt ttttctgaag aaccttctgc
    60121 tggagaggca gagtttatgc tatctcctcg caaatttaaa gatgtgaacg gtttaatggc
    60181 tgaacccgat aaaggtttta aattgaccac tgaggtgcta cgcagaaagc ctgattcagc
    60241 actaatttgg tatgaagact tcagagatga caatccactt ccctccagct actggacagc
    60301 actaagcggc gagtggcagg tatggcaaaa tcaaaatgat cccgccagtc gtccctattc
    60361 gcagcttgac gggtatggcc aattggcgtg gaagtatagt gggttttcag atgttcattt
    60421 gcgggcaagg ttggccttta ctccagaggg aagtggtcgt gcaggcattt tttgtggtaa
    60481 tgtcttttgc tgtttaaact ttaatacaca gcgtattgaa ctatataatg gtgcttcttt
    60541 aattggcagt tacaccactg aaatcgttaa aacggcatca gcagatcttc gctcaaatcc
    60601 aagaatgtac accattgaaa tgaggatacg aggtaactct gtgagggttt attctggagc
    60661 aagcaatgtt ttgcgcttta cagcgacgct tagtggtttt tcaggagggt atgccggggt
    60721 tcggtctgat ggacgtattc tatgtgaatt acttagactg ggagatgcct ggacttatga
    60781 accttatgaa aggtttgatg taaccttccc ggatggaacc acaactgagt atgggcggct
    60841 ggcaagaacc ggtgtaacat gggatagtga gtttcaggtt ttcacagtta acaatgatgt
    60901 ggaggaagct tctacacgaa accaggacat ctctatggat tatgatttct tccattcagg
    60961 tcttttgcca ttagtctgtg gaaatgatta ttcagtaaaa atcgttccca aggacatcaa
    61021 tgtctggatt tcacgtttgt ttcttggaga tgctgacggt ttttctattc tttattatca
    61081 ggatgtagac agccttgttt attgggcaaa cgaggcagcg tatcgatgga agcttagagg
    61141 aattgccata tggtctttag gccaagagga tatgaggcta tgggaggcac tgcctaaaca
    61201 aatataatta acgattcagg agtgtttgcg aaaaattgca agcactcttt ttatatattc
    61261 accaatcacg aaggaggtaa aaatcatgaa ggaaatatgg aatggggtac aagttgccct
    61321 tacagcactt ggtggattct tgggctggtt tttaggtggt tttgatggtt ttttatatgc
    61381 attagtggca ttgacgatag ctgattatat cactggtgtc atgtgtgcca ttgttgataa
    61441 gaagctatct agtgagatcg gatttagggg catctttaag aaggtgctta tttttgtttt
    61501 agtcggaatc ggacatatga ttgatacgaa ccttatcgga gaggggagcg tgctccggac
    61561 agccattatc tttttttatt gctccaatga aggaatatct atgttagaaa atgctggtcg
    61621 actaggattg ccaataccag aaaagttaaa agacattctt gtacagttgc ataacaaagg
    61681 aggaaatgag taatgaatct gaggaaactt attctaactg aaaatgcgtg ttataaggca
    61741 ggcagaaaga ttataccgaa gggcatcatg gttcatagca ctggtgcaaa taatccgtat
    61801 cttcgtagat acgttggacc ggacgatggc ctgttgggtg taaacgagta caacaatcac
    61861 tggaatcagg ataaaccagg tgggaggcaa gtatgtgttc atgcattcat tggaaagctt
    61921 aaggatggtt caattgccac ttatcaaacc ctgccatgga accatcgtgg ctggcatgca
    61981 ggtggaaagg caaatgatac tcatattggt tttgaaatct gcgaggacgg tttgaccgat
    62041 gcctcgtatt ttaatgcggt ttataaggaa gcggtagaac tgtgtgtgta tctctgcaag
    62101 ctctataact taaccgaaaa agatgttatt ggccactatg aaggatatca aaaaggcatc
    62161 gcaagtaatc atggcgatcc gaaaaactgg tttccaaagc atggaaaaag catggatatc
    62221 ttcagagcgg atgtgaaaaa gctattaagt gaaggagaga agcctgcaga acaggagaaa
    62281 aagaaatatt atcgtgtgca aatcggtgcc tattctgtaa aagccaatgc agaggcacag
    62341 cttgccaaag cgaaaaaggc tggctttacg gattcgttta taaagtatga ttaacacttt
    62401 tacctatgac ctgaggagtg taatagctct tcgggtcatt tttttttgac ctttaggggt
    62461 tcgaatcatt agggattttt gcatataggt gcagggtttt cactgtagaa atggaggttg
    62521 cctatatgca gataactaaa attacagata aacaagatca aataagcatt tctaaaaaga
    62581 cacttttaag tgctgaggcg cttcaaagag aatttgatta ttatagggca gaaaagttgc
    62641 tgagtaagat gctcgaaaaa ggcttaataa cggaagtgga attcaacaaa ataggcgcac
    62701 taaatcgcca aactttctct ccatttttga aggagataat gccctcgaat cgttgatata
    62761 taagggtttc agaggtaata tgtgacctac caagaaggag gtgagaggat gaaaaagata
    62821 acgaaaatag aaggaaatac ggccaactct tttattaagc caaaaacacg agtagttgcc
    62881 tattgccgag tttcaacagg cagtgatgaa caacttgtca gcctgcaagc acaaaaggcc
    62941 cattatgaga gctacataaa ggcaaatcca gaatgggaat acgcaggctt atattatgac
    63001 gagggtatca gcggcacgaa aaaggaaaac cgctctgacc tacttagaat gttatcagac
    63061 tgtgaaactg ggagaattga cttaatcatt acaaagtcca tcagccgatt tgcgagaaat
    63121 actacagact gcttggagat ggttcgaaaa ctgatagacc ttggggttca tatctatttt
    63181 gagaaggaaa acatcaatac aggttcaatg gaaagtgaat tgatgctctc cattttaagt
    63241 gggcttgcag aaagtgagtc aatttccatt tcagagaata cgaagtgggc cattcaaaga
    63301 cgatttcaaa acggaacctt tataatttcc tacccaccat atgggtatca aaacattgat
    63361 ggtcaaatga aagtaatccc taagcaggct gaaattgtaa agtatatttt tgcagaagta
    63421 ttatcgggca aaggtacaca gaaagttgca aataatctta atcaaaaggg tatcccatca
    63481 aaaagaggtg gccgttggac ggctactacc attcgaggga ttctgaccaa tgaaaaatat
    63541 actggcgacg ttattttgca aaagacttat actgacagcc attttaacag gcacaccaat
    63601 tatggtgaga aaaatatgta tctagtagaa aaccaccatg aggcaattat cagctatgag
    63661 gattttgaag ctgtagatgc cattcttaat cagagagcaa aagaaaaagg catcgaaaag
    63721 cgcaacagca aatatctaaa ccgatatgct ttctctagca aaattatctg ctcggaatgt
    63781 ggcagtacct ttaaaagacg gattcattca tccggtccaa gaaaatacat tgcttggtgc
    63841 tgcagtaagc atataagcaa tataacggaa tgttctatgc agttcatacg agatgatgat
    63901 acaaagactg catttgtcac gatgatgaat aaactcattt tcggtcagaa attcatatta
    63961 agaccacttt tgaatgggtt acgtaaccag aacaatgcgg caagttttcg cagaattgaa
    64021 gaattggaaa ctaagattga aaacaacatg gagcagagcc agatgctgac ggatttaatg
    64081 gccaaagggt atctggaacc tgccctgttt aataaagaaa agaattcact ggaagcagaa
    64141 agagaaaggc ttcttgctga aaaggatcaa cttactcgtt ccgtcaatgg caattttgca
    64201 aaagtagacg aagttgaccg tttacttaag tttgccacta agtccaaaat gctcacagcc
    64261 tatgaggatg agctgtttga aaattacgta gaaaagatta ttgttttttc acgagaggaa
    64321 gtcggatttg aattaaaatg tggaatcaca ttgaaggaga ggttggtgaa ttagatgggt
    64381 cacacaccct atggatatag aattgaagat ggaaaggctg ttgtggatga tatagcagca
    64441 gaaaaagtaa gagaattatt ttcagggtac ttagcaggac tttctttgaa gggcgctgct
    64501 aaaaaagctg ggctagactg ctaccatgcc acagcaagta agatgttgca gaacaagcat
    64561 tacttgggcg atgaattcta ccctccaatt attgatgaag agacctttga aaaagccaga
    64621 gtagaaaaac gaaaacgagc agaaaagctc ggaaggatat gggagcctaa agatgaaccg
    64681 gaaagggatt accctgtaaa gttcaaagca aaacctctgg tgcaaaaata tgaagatcca
    64741 tataagcagg cagaatatgc ctacagtttg atagaaagtg aggtctaaca agtggcggta
    64801 agtaggaatg taacagtgat tccggcaatt aaacgggttg gaaataataa aaatagtgaa
    64861 agcaaatcaa aaatacgagt ggctgcttac tgtcgtgttt caacggatag tgaggagcaa
    64921 gcttcaagct atgacattca gattgaacat tatacaaatt atattaagaa gaacaaggaa
    64981 tgggaattgg caggaatttt tgcggatgac ggtatcacag gtacaaatac caaaaagcgt
    65041 gatgaattca accgcatgat tgaagagtgt atggcgggca atattgatat gattatcaca
    65101 aaatccatca gccgattcgc tagaaacacg ttggactgcc ttaaatacat ccgtcaatta
    65161 aaggataaaa acgttgctgt gttcttcgag aaagagaata tcaacaccat ggattccaag
    65221 ggggaaatct tgctgaccat tatggcatcc cttgcccaac aagaaagcca atccttaagc
    65281 cagaacgtta agctaggtat tcagtatcgc tatcaacaag gtgaagtaca ggtcaaccac
    65341 aagcgtttcc ttggatacac caaggatgaa aataagcagc tagtgattga accaaagggt
    65401 gctgaggttg ttaaacggat ttacagagag taccttgagg gagcaagcct tttacagata
    65461 gcaagagggc tggaaacaga cggtattctt acagcggcag gcaaagccaa atggagacca
    65521 gaaacactga aaaagatatt gcagaatgaa aagtacatcg gtgatgccct tctacaaaaa
    65581 acatatacgg ttgatttcct ttctaaaaaa cgagtcaaga ataacggcat tgttccccag
    65641 tattatgtgg aaaacagcca tgagcctatc attccacgtg agctttttat gcaggttcaa
    65701 gaagagatgg ttcggagagc gaatcttcgt ggcgggaaag gcggtaaaaa gagagtttat
    65761 agcagcaagt atgctttatc gagtattgtt tattgcggtc attgcggcga tatttaccga
    65821 cgggtacatt ggaataaccg aggctataag tctattgtct ggagatgcgt tagccgattg
    65881 gaggaaaaag ggtctgaatg cactgcccct accataaacg aggaaacatt acagacagca
    65941 gtggtcaggg ctattaacga acttttggct aacaaagaac ctttcctttc aacattacag
    66001 aaaaatatcg acactatatt taatgaagaa agtgataatg atacggatga gcttgatagc
    66061 aagttggaag aattgcagat agagcttctt aaacaggcaa agtcaaaaaa tgactacgat
    66121 aatgttgctg atgagatata ccgccttcgg gagatgaagc aaaattcact tgtagaaaat
    66181 gccgagcgtg aaggaaaaag gcaacgaatc gctgaaatga ctgctttctt aaatgaacag
    66241 tctcacgagt tagaggagta tgatgagcag ttggtaaggc ggcttattga aaaagttacg
    66301 atccgcgatg acaggattga ggtggagttt aagtcaggtg tgaagattga agaaatgata
    66361 tagattattg agtcaaccgc aattaaggaa actatcgcct ttggcggtat ttccttcctt
    66421 tatatttttt gtgttgaata tagaacctta ttgtgataaa atatacgctg atacaaataa
    66481 gaattcagga ggaaccgaaa ctatgacagc ctcaatgcgt ttaagataaa aggtaatgaa
    66541 gtaattaaag attaaaggag ctaatatttt atgataggac cagataagaa aaaactttat
    66601 ccaaatgaaa atataaagac ggtttgtttt ataagcaatc tacctaaaag acccaatgtg
    66661 gagattgggg aatataccta ttatagcgat aataaaaaaa caccggagaa attttatgat
    66721 aatatagagc accattacga atttctaggg gataaactta taataggaaa gttctgtgca
    66781 attgcagagg gtgttaagtt tattatgaat ggtgcaaatc atagaatgga tggaattaca
    66841 acctatccct ttaatatctt tggccatgga tgggaaaagg tgactcctac aattgaacaa
    66901 cttcctttta agggagatac agtgattggc aatgatgtat ggataggtca aaatgttacc
    66961 attatgccgg gtattaaaat tggtgatggt gcgattattg ccgctaattc aacagtagtg
    67021 aaaagtgttg agccatatac aatatatggt gggaatccag ctaagtttat caaaaaacgc
    67081 tttagtgacg aaaagattga attcttgcta aagctacaat ggtggaactg gagcgaagag
    67141 gaaatattta ataatcttga aatgttaaca tccaaagcag ggttagagca attcatgaaa
    67201 aattcctaga cttggggcct ttctaccacg gcacaaaagg gaacttaaat ctagggggtg
    67261 ctggaatctc gatttagctc taattattgc gagctaaaga aggcaagctt catctttagc
    67321 tttgattgga acaatagaca ttatattatt aggagaaata gctttgagat ttatcactta
    67381 tagatttaat ttgaacggag ggattacatg gtaagtaaaa ctgatgccat tgagataata
    67441 acatatgctg aagaaaatga tattaacatt tggatagatg gtggttgggg agtagatgct
    67501 ctattagaag aagaaacaag agtacacaac gatattgatt tatttgtgga agaaagtaat
    67561 ggtaaaaagt ttattgaaat attaaaagaa tatggctttg ctgaagttat cgaagcatat
    67621 accaccacat ctcatacggt ttataaggat agtaaaggta ggataattga tcttcatata
    67681 tttaaattca acgaacaagg atacattgtt tttgagggag aagcatatcc tccagaagtc
    67741 tttagcggca ttgggaaaat aggcgataaa acggtaagat gtatcgatgc tgaaaatcaa
    67801 gtgttatttc atctgggcta tgagcatgat gaaaatgata tgcatgatgt aaaactattg
    67861 tgcgagagat ttaatattcc tgtaccgagt gaatacaagt aactgacaaa ttccgattta
    67921 attagaattt agaggaggaa aagatacggc acgttctatt aaagattccc tttctggatt
    67981 tggaagtgaa ctgttggaac gaataagaga agatagcaat gcaaggctag atgaagctga
    68041 ctttgaagaa agagtcattg ggatttatgc cttagcgaag caaaggaatt tttgcagcaa
    68101 gaaagataca ctgaagaaaa gtaaaggatt cctgttgtaa acatcccaaa gataaattta
    68161 gtttaacgat actaattaca tgtaagggaa tcgacaatac tgtgcacaga ttgtcggatg
    68221 gataaatgca tcctctgata atattgatga tgaaaggagg ttgtttgatg gattctttaa
    68281 gaagtatgaa taatgcattg gagtatattg aagagcactt aacagaggaa attgattata
    68341 ttgaagtatc taaaatcgca tattgttcag agtatcattt caagcggatg ttttcttttt
    68401 tagcaggcat aagtttatca gaatatattc ggagaagaag actgacgttg gctgcacttg
    68461 atttgaaaga tagggatttg agaataattg atgtagccgt caaatatggc tataattcgg
    68521 ctgattcatt ttcccgtgct tttcattctc tgcatggcat tctcccttct gaggcaagga
    68581 gtgagaatac acaattaaaa gcttatcctc gaatgacctt tcaattatca attaaaggag
    68641 gatgcgaaat gaactatcgt attgttgaga aaggaccttt taagttagta ggatttaaga
    68701 agagagttcc aattattttt gaaggtgtca atccagagat tgcaaaaatg actgaacttt
    68761 taaccatgga tgttattaat caattaaaag caatttcaaa tgtagaacct acaggtatta
    68821 ttagtgcttc cactaatttt tcagaaggta gaatggaaga gaaaggagaa ttagatcatt
    68881 acatcggggt agcaacatca agtaatgaaa ctgcagattt tgatgtatta aaaattgatg
    68941 ctagtacctg ggctgtattt gaatcgattg gaccatttcc ggaaacactt caaaatgtgt
    69001 ggggtaggat atactcagag tggtttccat cttcaggata cgaggcggtt gaaggtcctg
    69061 aaattttatg gaatgagagt ccagacactg gaaatccaaa gtatcgaagc gaaatctgga
    69121 ttccagtaaa gaaaaaagac tattgattac attaatgttt attattgagg gcgctcctag
    69181 taagagtgcc cttttcgtaa acggtacgat tggtggatat gttcccgtct accgatagtg
    69241 ccaaaaacgc agtggatatg tttccgagaa cggacacact tatatgacat tcattctgtc
    69301 ctctcgagcc attgtgagac tgtagtgttg ctacgtcgaa aataatgcta gaaatcttga
    69361 gctttagaca gtttcataga catataatat ttagattaga gggtaataaa attaggaata
    69421 aagaattgaa aaggtgtttt gatgtgtctg tagttatata tatggagtgt gggaatacga
    69481 aataaaaaaa gttttaatta tagaaataga tattaaattt ataaatttaa tatctatttt
    69541 ttatgtgaaa atatatttaa tctaaagaat gatatttcat aaaaaaaatt aaatatattt
    69601 ttaaaatatt tacaatagag ttatttttta ttcgtataat aatagaaata ttttagcata
    69661 agatgtataa taaaggaaaa aatacagaat taaaaaacat aagatagata agtggactta
    69721 ggggagagaa attattatgc aattgacaaa aataattctt agtaactata ggtgttttgg
    69781 agaagaacct acaactattg aaattaataa tttaactgga tttataggtc ataatagcag
    69841 tggtaaaact gctttattaa gtgcacttat gaaacttttt ggagaaaagt ctagtgatag
    69901 aattattgaa agatcagatt tccatatacc tgaaagtatt gatccagata gtatttctaa
    69961 gaatgaatta tatattgaag cacaatttga gtttcctgaa ttggagagag gtaaagacag
    70021 catatctata ccagtatttt ttaaaaatat ggttgttagt gaaccaggtg ggaaacctta
    70081 tttaagaatt agacttgaag caaattggga gaaaagctca aatcctgaag gaagtattga
    70141 ttcaaatgta tactatataa catctacaac agaggagata aatcctgaag ataagcgtaa
    70201 tatcaaaaga tatgagcttg atcaaataaa gatgatttat gtacctgcca ttaggagacc
    70261 agatgaacag ttaaaaaatg tctcaggaac aataatttca agattattaa atggtataag
    70321 ttggtcagaa caaacaaagg caagaattaa aacgaaagca agtgaaacgg aggaaatttt
    70381 ctttgaagaa aaaggggttg aatcattaaa gaaatcactc gaaactcaat ggaatagtta
    70441 tcataaggat attagatatt ctaatccaaa gataaacttc aatagtactg atttagagac
    70501 aattttaaaa aaagttgaag taactttttc acctacaggg acacatcgag attatcgggt
    70561 tgaagagctt ggagatgggt tacgttcttt attttatctt tcacttgtaa atacattttt
    70621 agaaattgaa gaagaaattc taaaatctat taatgatggt aaagaagaag atatatttac
    70681 attaataccg cctgtattaa caattgttgc ggttgaagaa ccagaaaatc atatttcacc
    70741 tcatctttta ggaagggtag ttgaaaaatt aaattttatt tctacaaaag aaaattcaca
    70801 gacaatattg acatcacatt caccagcaat agtaaaaaga attgaaccaa cagacataag
    70861 gtattttagg atttgtaagg aatatgaatg tacaaaagtg aagtcaatta tattaccaaa
    70921 tgaagaaaag gaagcagaac aatttaaatt tgtaaaggaa tcagtattag catatcctga
    70981 actttatttt gcaaaacttg ttgtattggg cgaaggtgat agtgaagaga tcattattaa
    71041 gcagatgatt gaattaaaag cagaatctgt agattcatca gaaatttcaa tagtaccatt
    71101 aggtggtagg catgttaatc atttttggcg tttattaaat gatttagaaa ttccatttat
    71161 tactttgtta gatttagata gagaacgaga tggaggtggt tggggacgta taaagtatgc
    71221 aatacaacaa ttaataaata ttggtcatga taagaatgta ttactttata catgtgaagg
    71281 aatttcacaa gataatgaat ttaaagagat gcatacttgg gatgtaactg atactgaaaa
    71341 tatggctaaa tggattgaaa tgctaaaagg ttatgatgtt tatttttcag ctccgttaga
    71401 tatagatttt gcaatgttac aaagttttaa agataaatat atttcagcat taacaagtgc
    71461 ggaaggtcct aggataaaag atcatggaaa aatacaagat attgtgatca atgaagatgc
    71521 tgaagatgaa tcattgaaag ctgcttataa agatagaata gatagtgata ttaaatcaac
    71581 cttaaaacaa gaaggtggtg atggatctac ttatactgag ggagagaaag aactaatgat
    71641 atggtattca tatttctttt taggaagagg taaaccaact actcatttat ctttattttc
    71701 taataatact gatatagatt ttgaaagttt tccatactgt ctaaaggagt ttgtaaatag
    71761 tattattgaa aagatgatag atgattcagg gggtggaaga aaatgaagag gaattgggtt
    71821 agtaaagaag attggagtcc agcggctgga ttcagactag aagttgaagc gatgaataca
    71881 gttattaatg ataagaatac acttgttgta gctggtcctg gcgctggaaa aacagagtta
    71941 ttagctcaga gagcctgctt tttactagag actaatacct gtagatatcc taaaaaaatc
    72001 cttgctatta gttttaaaaa ggatgctgct gaaaatttaa aagagcgttt agaattgaga
    72061 tgtgggaaag aattagcttc aagatttgaa tcaaagacct atgatgcttt tgcaaaagaa
    72121 atcgtagata gatttagaaa tagtttagat gaagcatata gacctttaaa taattacaat
    72181 atagctgaaa atcgtgatgt tagaagagca tttgagatag caggattaaa tttcagagga
    72241 aatcaaactg aatttaataa agtatatgga aataaactat cgataaataa attgccttta
    72301 catacagatt ttgtagataa gatttttata gatgcttgga atgtactact taaaggcaac
    72361 aaagatacta attttgtaag ttcacttact tttggtatga tatcacgttt agccgaatat
    72421 ttgataagaa ccaataggta tattaagaaa gcaattgaaa tgacttatag tcatgtgttt
    72481 ttagatgagt ttcaagatac taaatctatt caatatgatc ttgttaaagc ttgttttttt
    72541 aaaacagaaa caataattac tgctgtaggg gatggcaaac aacgaattat gttatgggct
    72601 ggtgctagaa agtcaatatt tgaagatttt caaaatgatt ttttaagtca aaaaagaaca
    72661 ttaattatga atcatcgttc agcgccaaga cttgtagaga ttcaaaaagc aatgtataac
    72721 agtttgaatg aagaggcaat tgatttaaaa actaatgaaa agtggaatta tgaagatgga
    72781 gaagtgtatt taagactttt tgaggattat ataaaagaag ctgatatact tagtgaggaa
    72841 atagtcgaat tggtggaaac aggtatagaa attaatgaaa tatgtatatt agtaaaacag
    72901 agcgtagata tttattcaaa agagattata aataaacttt ctgaacgtaa tattaaggcg
    72961 cgtaatgaag caatgtatca ggagatatta aaagaagata taataaagac actggtttcg
    73021 ctttttaggt tgtcgttaag tgatagaaat cccgatgatt gggattatat ttacgatatt
    73081 ctaggtgaat tatatggaat agacgaaggg tataacccta aaatattaaa tgatttcatt
    73141 accaaagtag aattaatgat taatgatctc aaggtaaatt taaatattac tgatgaaaaa
    73201 caattttcag atttagttga ttctaaggtt gaatatattt catatgaaaa atttgcatca
    73261 aagtttcaaa aatataaatc taaagattat tttgatgatt tggtagataa atttaaaaag
    73321 ctggtatgga ttgaatataa tgaaactaaa gattggatta aagcgattga taactttgaa
    73381 ggtaaaaatt caattccaat aatgacaatt cataaaagta aaggacttga atatgatact
    73441 atcttttttg ttgggttaga agattcagca ttttggaatt ttaaaaatca accagatgaa
    73501 gatagatgtg cattttttgt tgcattatca agagctaaaa gaagaattga ttttacattt
    73561 agcttagata gaccagcagg attttcgaat tcacagaagc attattgtat taatgaattc
    73621 tacgaattgt ttgataatac tgagattgta aaaaagatag agtattgatt aagatgataa
    73681 aaactttagt atattatatt ttaagatatt agttatataa gtagacattg aatttatgta
    73741 tttgatgtct attttttata taaaaatata ttaaagaaag gatgaatttg catgaagaaa
    73801 aaaacaaaaa tatctaaaaa gattgtaagt tttattatgg tttttataac tatagttact
    73861 actatactta catcatcagt ttcagcttat gctagcgagt tggatatgag tgaacagaca
    73921 ggctttaatt atttaggtat tagtccaatt acaggaaaaa aattaatcat aacatttatc
    73981 gtatgaagat ggataataaa attgtatttt gtgtagagcc aggaattaaa acttcaagtg
    74041 gaggaggata tatatcggag gagttaggag ataaatttat atctacaaca cttccaaatt
    74101 atcataaaag aaaagctgaa atacttgaga aggtaaatag acataatata ttaccttcat
    74161 ggaataatga aaaagttgca gttaaaagtg gagaaacaat tgagttagaa gataagaatg
    74221 gtattattag agaaatgcaa ttagaatcaa ataatactgg aacagatata ttacttgaag
    74281 aaaatacttt gaaaattaca gcaaataaaa attctaattc aggagttatt agttatataa
    74341 agattcctaa aaatgaggtt ggaatatcaa tagtttatcg taagcataac tatcaaagct
    74401 tatcagaatt tcatatggaa agttcgatga gtgcaaattt aaatataaat gtaatcaagt
    74461 taggaattgt taaagtaaaa aagattgatg aaggcacagg aaaggaatta gcaaatgcaa
    74521 aacttagatt tgagtatgat ggtgatagta aagaaattat aacgggttct aatgaaattg
    74581 ctagtataga agatattatt ggttgattaa aaagtgaagt atacttttgt atctatgaaa
    74641 aagactataa gcaatatata aaattagata tacctattga tgaagcaaaa ataaaaaata
    74701 gatttgaaat aaggttaaaa aatgaaagag catatcatgc agcagttgat ttgttagcaa
    74761 atagagatga agaaaagaca gcattttcaa ttattaatcg ttatatacgt tttgttgata
    74821 aagatgatga aaaaattaga agtgaatgga aaactaatga tcagtggagt tattttattg
    74881 gtgaaggtag agcgaagtta aaactaacaa caaatccaga accatataat attcaaaaaa
    74941 ctttaaattg gttatcaaga caagttgcac caacgttaaa aacaattaaa aggttagata
    75001 gtttgaacaa ctcaacatta attgagggct taataaggta tgcaagacta acagataaac
    75061 ataaaaaaat aattgaacaa aattcagtaa atgttgaaga cattatttat gatgaataaa
    75121 aaaatgtaat tagtattgca gattttaata taaaagaagg gagatatacg aatgaatttt
    75181 ggtcaaaatt tatataatta gtttttatca aatgcacaat cattggtatt aatggctata
    75241 gctataattg gtgtatattt aggatttaag agagagtttt caaaacttat aggattttta
    75301 gtagttgcat taattgcagt agggcttgtg tttaatacag caggagttaa agatatatta
    75361 ttggagctat ttaataaaat aataggtgtg taaggattaa aagagaatag aaaattatga
    75421 gtatatgatg aagggttcta ataagtttta ttaaaatata tattgaaaga caagtagagc
    75481 tatttcgagt tattacatgt aatgctaata tatataactg cattttggaa attaaataca
    75541 taattagaaa tagcaaattt tataggtgac ttattaatat gtttacttat tgtatactat
    75601 ttgatatatt taatatgagg tgattcttga aaatggacat agtgaaagtt tttgctaaca
    75661 atgtgagaaa atatagaaat aaaaaaggtc tatcacaaga agcttttgct gaaaaagctg
    75721 gattgcatag aacttatatt tgtgctattg aaagaaaaaa gagaagtatt gctttagata
    75781 atgtgcagaa aattgctgat gcgcttgaga ttgagactta cttactattt atagaaggtg
    75841 aggagaaaga atgtctatga aaaaattacc aatagaaaag cttatagaaa atttaggttt
    75901 ttcagggtca aagaatcttg tgaatataga tatgcttaca aaattagata tatctaaaca
    75961 tgatgtaaaa actttaaaag ttataaatcc ttatgcaatt tatttagtaa atagtaagcc
    76021 atttatatta tttatagagt cttcttttga agaaaaaaaa ttacaacaaa ttagtaagct
    76081 tatatggaat gctcaaatac caattgcttt tatatgcgat tctaattctg ttaaaatatt
    76141 taatggaaaa gctttggatt taaagtcata tttaattg
//
LOCUS       DAGSHT010000436        18255 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.guided.058, whole genome shotgun sequence.
ACCESSION   DAGSHT010000436 DAGSHT010000000
VERSION     DAGSHT010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.guided.058"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..628)
                     /gene="bclA1"
                     /locus_tag="MJB72_003876"
     CDS             complement(<1..628)
                     /gene="bclA1"
                     /locus_tag="MJB72_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBY8264904.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTG"
     gene            complement(1306..1830)
                     /locus_tag="MJB72_003877"
     CDS             complement(1306..1830)
                     /locus_tag="MJB72_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY8264905.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGMDYSDVEKN"
     gene            complement(1895..2647)
                     /locus_tag="MJB72_003878"
     CDS             complement(1895..2647)
                     /locus_tag="MJB72_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY8264906.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(2678..3976)
                     /locus_tag="MJB72_003879"
     CDS             complement(2678..3976)
                     /locus_tag="MJB72_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBY8264907.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(3993..6257)
                     /locus_tag="MJB72_003880"
     CDS             complement(3993..6257)
                     /locus_tag="MJB72_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBY8264908.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(6512..7330)
                     /locus_tag="MJB72_003881"
     CDS             complement(6512..7330)
                     /locus_tag="MJB72_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY8264909.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(7323..8021)
                     /gene="cbiQ"
                     /locus_tag="MJB72_003882"
     CDS             complement(7323..8021)
                     /gene="cbiQ"
                     /locus_tag="MJB72_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBY8264910.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(8024..8308)
                     /locus_tag="MJB72_003883"
     CDS             complement(8024..8308)
                     /locus_tag="MJB72_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY8264911.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(8298..9050)
                     /locus_tag="MJB72_003884"
     CDS             complement(8298..9050)
                     /locus_tag="MJB72_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBY8264912.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(9232..9424)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9556..11328)
                     /locus_tag="MJB72_003885"
     CDS             complement(9556..11328)
                     /locus_tag="MJB72_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBY8264913.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSIYSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(11342..12271)
                     /locus_tag="MJB72_003886"
     CDS             complement(11342..12271)
                     /locus_tag="MJB72_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY8264914.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            12499..12687
                     /locus_tag="MJB72_003887"
     CDS             12499..12687
                     /locus_tag="MJB72_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8264915.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            12680..13381
                     /locus_tag="MJB72_003888"
     CDS             12680..13381
                     /locus_tag="MJB72_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264916.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            13383..14306
                     /locus_tag="MJB72_003889"
     CDS             13383..14306
                     /locus_tag="MJB72_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8264917.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            14463..15386
                     /locus_tag="MJB72_003890"
     CDS             14463..15386
                     /locus_tag="MJB72_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264918.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            15379..16116
                     /locus_tag="MJB72_003891"
     CDS             15379..16116
                     /locus_tag="MJB72_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264919.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            16127..16900
                     /locus_tag="MJB72_003892"
     CDS             16127..16900
                     /locus_tag="MJB72_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264920.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            16916..17068
                     /locus_tag="MJB72_003893"
     CDS             16916..17068
                     /locus_tag="MJB72_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264921.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(17441..18049)
                     /locus_tag="MJB72_003894"
     CDS             complement(17441..18049)
                     /locus_tag="MJB72_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBY8264922.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5928 a         3123 c         2260 g         6944 t
ORIGIN      
        1 ctcccgttgg tcctgtactt cctgtcgctc ctcttggtcc cattggacct gtaattctaa
       61 ttggaagaca ctcacattta ggataacaat tatctataat tctatcttca aatgttacaa
      121 gtatatacgg tttgttacaa cctctactag atgtaaattc aagaatctga taagggtaat
      181 ttaaagataa agctaaccca taattaggat atttattcat tgcccatgct tttatagttc
      241 ccgtaatatt taaacaaata tagttggatg tatcacttat cccaattggt aaatatcccc
      301 tgatattttc catagacact ctatcattcc atgttatagt tcgtaaatta taatattcac
      361 ttattctttt aatttcaata tctacttttt ctttaaaaat atgattctta tttgaatcaa
      421 tatgaataaa taactctgct tttaatatac ttttgtcttt taatatatct ggtattctca
      481 ctttaaaaaa agtaatcaac ttttctttta caaaactatt tgaacattta cttcctatta
      541 agcaataatc taaattacta aagtttttat ctggatattt tttagatata aaagtatcat
      601 catttaaata aagtataata tttctcattt ataatccctc cattttgggt ctatatcata
      661 ctatgtttaa aatacatgct tagttacaat acaattaaaa tttatagtat taaattttta
      721 tataataatg ctgatttcta caaaaaaaga acatgtcatt agaaatttct aatagcatgt
      781 tctttttcat tattatatat tacactttaa attattcctt gactggctta gctggtgtaa
      841 actaaaccag tctttctaaa atattaagca cttttttctt attatgtttt ttaggattta
      901 ttgtctatat tattgtaaac caaagttata aaattgtaaa tagaatatat taccataaca
      961 atattttttt ataagtaaaa attataaaaa actttatttt tgttaaatag aataaataaa
     1021 ataagtttaa aaaatactga aataagtgtt ttccacatat tttatatatt tatttaatac
     1081 attattattt aaaaataaat atataaacaa tcataataaa atttttttaa taaaacttta
     1141 ttttttagtt aaaatcatac aatatataga tttccacaat acaaataaat taaaaagtga
     1201 aatgtatttg tctaaaaaca taataacatt tcactttatc ctcattttta agtaatatat
     1261 atttttaatt tttatttata actaaatcta gtttttatat aaattttagt ttttttctac
     1321 atctgagtaa tccattccaa aagtttctac agtaacagat ttcataactt gtggagtttg
     1381 tggcttatca gctgaatctg ttctaactga tgctatttca tcaactgtat ccataccttc
     1441 tatcactcta ccaaaacctg catactgacc atctaagtgt ggtgagtttt tatgcattat
     1501 gaaaaattga gaaccagctg aattaggtgc catagttcta gccattgata tgactcctct
     1561 ttcatgtttt aaattgttag taaatccatt accactaaat tcacctttta ttgagtgacc
     1621 tggaccaccc attcctgtac catttgggca tcctccttgt atcataaatc ctggtattac
     1681 tctatgaaat attattccat tgtaatatcc ttcatttact aatgatacaa agtttttaac
     1741 tgtatttgga gctatatttg gatataactc aattttgatt tcttttccat tttccatttc
     1801 tatagttact ataggatttt tattttccat aatttattac cttccttttc attagttaaa
     1861 ttacataatt acatttatta tattatactc taaattaaag tctatcaagt atattttttc
     1921 ctatttcata tccttcttga taaatagttt tacaaaactc atcatcaaag ttaagcgtgt
     1981 ctttaggagc aaattgagta ttaggtctaa ctactattat tttatcttca tcatatactt
     2041 ttttaatatc ttctggcaac tcataattat attttgtgga tattaaaata attttatcca
     2101 cattatcccc aattaatggt tcaattaatg cactcctaat taaacctcca tctaatttga
     2161 aaccatcata aattccttct tgcacatctt tcacaaattg ttctttaaat ccaagagttg
     2221 cgtttggatt tgatggtaac aacccactgt atgaaatgct gataatggct tcattacgag
     2281 gcatttttga tacgtcaaca atcttttctg atactacttt attatcaact tcaacataat
     2341 taacataaaa gttcatttct tctatattac tatcatctag ttcccttagg ttgtctatag
     2401 caggagaatt atccacagta ttatttacta tttttacatt tccattctgg atatctttta
     2461 tatttgtcca cattttctcc aagttatcaa cattccctgt gtacaaataa tatccattta
     2521 ttgctcctat tgatgtccct gaaatcgaat aaaatttatt tattcctcca tcatacaagg
     2581 ctttaactac tcctgcttga taagcccctt ttgctcctcc accttctaaa cataatccta
     2641 atttcatagt ctcatctcca aattgtttat cttatattta tatttcaaaa aacttactaa
     2701 atgttttata tatactatag tggtcctcaa catttcctaa ttctcttata gaatgcattg
     2761 ctaaaattgg acttcccaca tccacagatg gtatatctat atgagtagaa gatattggac
     2821 ctatagtact tcctcctctt tcatctgacc tattaacaaa ttcttgatac tcaacattag
     2881 cttctctaca tatattttta taaatagcta tagaatatcc atcactagta tatgcctgac
     2941 tagcatttat ctttataacc ggaccttttc ccattattgg tttattagtt gggtcatgct
     3001 tttctggaat gtttggatga atagcatgag ctaaatctga tgatatcata aaagatgaat
     3061 aaatagactc aaagaatccc tctctatcct tacccattga tatacaaatt ctctccaata
     3121 tatttaacaa catgtttgaa tctgcacctt gtttagtaga gctaccaact tcttcattgt
     3181 caaatacagc cattacattt acaccatgtt gacctttagt attaacaaga gcattcaaac
     3241 tagcatgtac cattgcaaga ttatcttgtc tcccaataga tataaattca tcattagccc
     3301 ctattaaact tcctttttca aattcatata aaaataaatc aaaatctata atatcttcca
     3361 tatttacatc taagttagat gctatttgct ttataagaaa attatctttt tctaatgtat
     3421 catttaacat cccaactaaa ggtaacatat ctttttgctt attatatgaa tgaccatcat
     3481 ttatacttct atttaaatgt attgctagat ttggtattat acaaattggc tttttaaagt
     3541 ctataagcct ttcatttggt tttaatatac tatcaccttt taaaactact ctaccagcta
     3601 tcccaagagg tctatccaac catgtactta atattgctcc tccatagcat tctgtattta
     3661 acttcaaata agatttttca gcttctattt ctgcatttgg tttaatacta aatgtaggag
     3721 aatctgaatg agagcctatt attctaaatc catctctttc tataccttct gaatttacta
     3781 caaaagctac tatagctgat aaattcttag taacataata ctttccacca actcttagat
     3841 tccatctttg attcaaaact aattcttcaa atccattttt atctaagatt tccttagtac
     3901 tctcgactgc atgaaaagac gacggactat catatatgaa gtctattaag tttttagcaa
     3961 agtttatact atccattcct catttacctc cattataaaa tatttagtgt tgtatattct
     4021 ttatttattt ttttaattcc ctgttggtca aaagctatag taacatctgt accaattata
     4081 gaaaccactg tcccaacccc aaatttagga tgatgtacct tagaaccaag ttttatatca
     4141 tctggattag tttctttatt agaatctttt attgtagcat taactttatc tgcaacttta
     4201 gatttgctta tagtactcat atacttttta gtatatttat ctaacatatt ataacttgct
     4261 ttagaatagc ttagttcttt ttttacttta tacaatcttt ctatacattc ttctggcagt
     4321 tcttccataa atcttgaagc tattgcaaca tttgttttac catataatgt tctcttctct
     4381 gtcaatgaca tatatagctc ttctttagct ctagttatac caacataaca aagtcttctc
     4441 tcttcttcta tttgtgaatc actcatagat tttatagctc ttgatatagg aaataaccct
     4501 tcttccatac ctgttaaaaa cacaacaggg aactctaaac ctttggatgt atgaattgtc
     4561 ataagcgaaa ccctatcatt gtcttcttca tcatttgatt cagatgtaag tgctatactt
     4621 gttaggaatg tctctaagtc tttattttca taagtatcat cattactact ttcaaactcc
     4681 aaagctatag aaataaattc tctcaaatta tcaattctgt cttgagcttc ttcacctttg
     4741 cctgttaaat ctttttcatt tttatttctt atctctacaa gctcgtccat atatcctgta
     4801 gtatccaaga ctttttctat aagtttgcta acaggataaa cttcttttat agttctaaga
     4861 gtccctataa tatcaacaaa tccacttata cttgcccttg ccttagtaga tatatctgaa
     4921 tttgtttcta tatcaattaa tactgagtat atactttcct gttttaagtt agctctatct
     4981 tcaatttttt ctatagttct aagccctata cctcttctag gaacatttat aatccttttt
     5041 atagatatat catcttgtgg attttgaata actcttaaat acgccaacaa atcctttatt
     5101 tcttttctct catagaattt tgtaccacca tatatattgt atggaatttg acttctattt
     5161 aaagcgtcct ctacaggtct tgcctgtgca tttgctctat aaagtactgc aaaatcctta
     5221 taaggtctat tttcttttct agctatttgt gcaattgaat cagcaatgaa atcagcctct
     5281 tctatttcgc taccagttaa ttgtatcttt ataagttctc cttctttttt ttcactccaa
     5341 agcttttttc tttttctttc tatattatta gatatgactt tattagctgc atctaatatc
     5401 acttgtgtag acctataatt ttgttctaac tttacaacat gtacattgtc ataatctttc
     5461 tcaaattcaa gtatgtttct tatatctgca cctctccatc cataaatact ttggtcatca
     5521 tctccaacaa cacaaatatt ttgatgttct cttgctaata attttatcaa ttcatattga
     5581 gctttacttg tatcttggta ctcatctacc attatgtatc taaatctact tctgtaatat
     5641 gctaacactt catcattagc cttgaaaagt tctacagttt taagtattaa atcatcaaag
     5701 tcaagtgcac tatttctttt taaacggtct tgatatagcg cataaatatc tgcaatctta
     5761 accattctat tatctgccat attgatatct ttaaactgct ttggagtata aagtttgtct
     5821 tttgctcctg aaattgcact tataactgct tttggttcaa acactttatc acttaaattt
     5881 aattctttta gacagtcttt aaccaatgta acttggtctg ctgaatcata aattacaaaa
     5941 cttctattgt aaccaatctt atttatatct tttcttagta tacgaacaca gcaagaatgg
     6001 aatgtactta tccacatatc cttagtttca ggacctatat tttgttcaac tctctctctc
     6061 atttcatttg cagctttgtt tgtaaatgtt atagccaata tatttggtgc ttgtacacct
     6121 ttatcttcta taagatatgc tattcttgtt gttaatacct tagttttacc tgaaccagca
     6181 ccagccagta taagtactgg accttcagtc ttttctactg cttctctttg agcaggattt
     6241 aatgtatcta aattcatatt tctcctcctg ttaacagcca taaatatatt ttaatctaaa
     6301 atatgaatta cttcaaatta taattatgta ttatattaat agattatttc caatatatta
     6361 caattaaata acatttttcc atataattta tttcattgtt taataaaaaa aataacctta
     6421 tgatttgggg aatcataagg cacctttaag gggtctaggt taagggttat tatatataaa
     6481 tctatttttt taaaataggt ttagggctta attatttatt tgtattaagt tctctttcct
     6541 tccaatactt atacaaatct tcttctttct taaataaggg taaattcata tttctatgta
     6601 cttttactaa ccatggggat tctagaccag cttgtataat attattttca tttataaaca
     6661 cattttctgc ttttccttca tcaatcaaaa tccccttatc caatacatat atgtaatcac
     6721 atatttcata cattaaattc atatcatgac tggatattac tatttttata ttatttttat
     6781 ttaatccctt tattatatct acaatagacc tagtacttac tgggtctaaa ccagctgtag
     6841 gttcatcaag taatacaatt tcattttcca ttgctataac tgatgctatt gccactcttt
     6901 ttttctgtcc atagctaaga aaatgtactg gcctgtctat aaaatctatt ccattaacag
     6961 ctactagagc tttgtttatt ctctctttaa ttgttttttc atccatacca atgtttctca
     7021 ttgcaaaggc tatatcatca tatacttttg aataaaaaat ttgtttttct gggtcttgga
     7081 acacaatacc tacatttttt ctaaggttgt ataaacttct cttatcatat tttaactttt
     7141 cttctttaaa aagaatttct ccagatgtag gctttaatat acccattaaa ttcataaata
     7201 atgttgattt tcctgaacca tttgaaccta ttatacctat aacatttccc tttgaaaaat
     7261 ccatattaat gtttaatagt gcattggtat ttttttcata ttgatacgtt aaattattta
     7321 ttttaaacat cattatcccc tactatgtga aatttaccat catacaattt tatgtctaat
     7381 gatatacaca tatcatcata ccttgtcata agtcttttgt acagcatact tccaagaata
     7441 cctaaggatt tatatgaagt ttttaaattt atatatccaa atcttagctc ttgtgacttg
     7501 tatatttctg aaaattcttc taaaaatatg aatataaacc tatatattaa cattgataac
     7561 tcgacaaaag tgtcaggtaa atgaagtttt ttaaaaaaga atattaattg attaaaaggt
     7621 gttgtaagta tacaaaaata cacacaagtc aaacaagaca tcgctctaaa taacacatga
     7681 atagacatgt caatagacgc ctttgatatt cctacattaa aactaaaaaa ttcaaaacta
     7741 tataataaat ctgctttatt aaaagatata tttacaagag ttattcctat actaagaaat
     7801 aaaaagtaca ttggtattct aagtaatttt aaataatttt ttaaatctat tccagccaca
     7861 caaacaacca aaatactcat taaaatcata attccactta aaataaatat attttgtatt
     7921 accatagaag caatcagaaa aattactcct atggctactt ttttgtttgg atttacttta
     7981 ctcaatctat ttgtatatgc atatttatca attataagca tgattaactt ctctttcctt
     8041 tttgaagacc taatatgtat cctattacac cagcccctaa agctgcttgt gcagagaata
     8101 ataaactttc tatttctcca cttggtggct catatggact gctaaaccat ggtttgtaat
     8161 ttggatttat cttagtaatt tcactttcag cttgaccatc tgctcctcca tattcagcac
     8221 ctgagttgac aagtaatggg aatataataa gagctactac taataaaagt aataatatgt
     8281 tagtttttgt ttttgtttta gcactcatta gctttaactc ctttatcatt attttctagt
     8341 atgctttttt ctccgttttc tgtaattaaa ttatatacaa ttacagtaag tataccttct
     8401 gctattgcta ttggtatttg agtcataaag aaaattccta aaaatttaat tgctgaagcc
     8461 attaccccac ctgatgggtc tgggaaagcc agtgctaatt gtattgaagt tattgtatat
     8521 gtggctaagt ctcctattgc tgctgcaaga aatactggaa tagcattatt tccatctttt
     8581 ttctttaaag ccttaaatat taggaatgat acgattggcc ctattatcgc cattgaaaaa
     8641 gcattagctc ctaaagtagt tattcctcca tgtgctaata acaatgcttg gaatattaaa
     8701 actatagttc caagaacaaa cattacgctt ggtccaaaca ttattgctcc aagacctaca
     8761 ccagtaggat gtgaacaact tcctgttact gaaggtattt ttagtgctga taatacaaac
     8821 acaaatgctc cacataaagc taataatact ttctttttag ggtcttgctt tactatttta
     8881 cctattgatt ttaatccgaa taagaaaaat ggtataaata taactcccca tgctatagac
     8941 cattttactg gtaagtaacc ttccattata tgcattgcat ttgatgtaac tggagttaaa
     9001 atgattaaca taagtgcagc tataacccct aatttaatat tctgtttcat cttagtatcc
     9061 ctctctccct ttctaataat taaaacataa taatacatgt ctgatttatt taataaaata
     9121 aaaaagccta actcgatggt taggcttcta attttccaca atctaaatac aaacacataa
     9181 aacaaacata tctgtataat tattataaga aactttctcc atccccgaag aaattacttt
     9241 aatattttag gcaggtctcc tgacttagct tcatcctact ccttttcctt cccatgagat
     9301 ataaaaaata tcatcatagt ggctaacaaa agtttcgtct gcatcacagt agcgggggct
     9361 gtaaaggaat ttaacctttt tccctattaa tcttgcttaa agaacctata atattatata
     9421 tttaattttt aaattattct taaattattt tctatataaa tcattgtata ccttttctgt
     9481 gaaaatttct acatttttta taaattttat tcatttttat atttttatgt atataaattc
     9541 ttttttgtaa gtttattaat ttctaattat cttctttaaa tacattgaaa ctacatcaga
     9601 aaatctagtt atatctcttg aatatataaa atcatttcca tatattaatc tttcagcttc
     9661 aagttctgat tcttttcctg taaatactcc taatactaat atattttgct ttctagcttt
     9721 tctcacttca ttggctgtat ctctcaaacc aactataccc ctataagcct tttctcctct
     9781 taatgttctc tcactacttt ttcctatctt tacatcattt ggtcttccat cgctgagaac
     9841 tatcaggatt ttattttctt catctctatt tattagacca ctacatatag attttattgc
     9901 caaaccatct ctattattac cagcacaaaa atattcaaat atattttcac tattttttat
     9961 actatcatca taatctttaa atctttttaa tattgtatag tctaaaaaac tagaaaatcc
    10021 catcactcta tttggtatgc caacttctgt tagagcattt gcaattatgt aagcttgaat
    10081 tgccacattt ccctgatttc tactttgaga gccacttgca tccaataata tatcaataac
    10141 gtatttccct ttttcatttt ctatatcttt ataaaatatt ttattattat tacttctacc
    10201 aactctccaa gctctatttg ctaatatagt accacaatca gaatatactc tacttttagc
    10261 attttcttct attaaaattc ttgacagact ttcttttaat ctctttattt gctgtctata
    10321 cattctagca tgttctctaa attttcctaa attattttct ttttgtcttg ttacatattt
    10381 tagttgaaat acattattac actcacttct aagaactcca tctgtaaagt gtattctaca
    10441 accttcatga acatttctac aattttgtgt ctcaattttt ttaatttcac ctttactcaa
    10501 ataagttttt ccataataat gttctatttt tgaatatatc ttatttgcag tgtcttcatc
    10561 aacatatata actcggtctg ctgaattgct acatttagaa tcatcaaaat cccctacacc
    10621 ttctacaagc atggatgaat atattgaatc tatatcactt tcaataatat tttcttcttc
    10681 atcactataa agttcttcat acatgaaatc agcaaatgta tcaaaattta cttctacact
    10741 ttctacatta ttttcatcaa taagactatt ttcttctcca tttaatatgt attcaaaata
    10801 agttaaatat ataacatcta atgcttttaa aatctccata atatcttgat tggtatctat
    10861 atttcttata tcattaacta ttcttctagt catactatct aatgctgggt gcttgtccat
    10921 actataaagt acaaatgtgt atttaatttt atccataata ttatcattgt gtattttggt
    10981 aaaacgtgaa acaatatctt tataagcatt tcttcttatg tcttctatcc ctggcctatc
    11041 tttaatgacc ttttcttcaa ctattgtatt taatatgact tgaattagac ccagtataat
    11101 atctttgttg tatccttttc ttattctatt tgtaagatat tttttaacta tatcccaatc
    11161 tacatacttt cttctagcac ctgcaattat tgcgaaatat agacctattt cttttgaaga
    11221 gtaatctttt tctgatatat ctatactttc attgtaatca ccagctattg tccatgatag
    11281 gttattagct ctattttcaa attcataatc atcatatatc catttcataa tctcacctat
    11341 attaaatatg acttattgga aacacatcta tactctccca tttattaggt attctagttt
    11401 taattacatc tccaaccatt tctttctcat atacatcaaa agtctttcct gtaagtccca
    11461 tattaattgc aagagttggt tttaatcctc ttcttatagt cttaagagat gccataagac
    11521 cacgtaaatc aatagcttta ctagatatct ctccattttg agacttcaat tgtaaatcta
    11581 aaaatattcc tgcaaagtgt attaattttt cttcatcagc atctgaaaat tcatttttta
    11641 aaatcatcat taacttatct tcttctatag gaggaatatc tattaccata aatctagata
    11701 ccagtgcttc attaagttct tttgttcctg catattcata attcatagtc ccaataaatc
    11761 ttgttgctgg atgtagattg actctctcat acccaggtac atcaattatt cttctatagt
    11821 ctaaagctga atgtaccaca actatagcat cattttttgc catattaatt tcatcaaata
    11881 ccccaaaacc tccattaata gcacactcat aaactgagcc tcttctaagt ttaacttcat
    11941 tgtctataaa tgtatctgta cctataagtg ttgaactgtc tgtatttata tgaaatgatg
    12001 tattccactg aggtctatta aatatttccc ctagattatc agcaagtaag ttttttcctg
    12061 ttgcctttgg tcccgataat aatatatttt cttcttctaa aatagctgct atacacatag
    12121 acaagatatc tttaccataa aaataagttt tagactttgt tactctgtat tctaaatctt
    12181 tttctaatcc ataataattt ctaaaataca gtacatcttc aataagtttt tcatctaccc
    12241 cttgcaccct tagattatct attatatcca tatttttacc ccttttatat aaatagtatt
    12301 tgtttcattt cttatcacca ttttagcata aaaggatata agctaaaatt gattttttaa
    12361 tctccccatt ttaagtaaaa ccatgcacca ttttatcact acaaaattaa aagtactact
    12421 tactgatatt tcttgatttt gttcgtctat acgtatataa tatatgctga taaattatga
    12481 ggaaaggatg attgaaatat ggaatatatg tcttgcccag aagtagcaaa aaaatggggt
    12541 atttctgaaa gaagagtaca aaaactatgt gaagaaagtc gcataccagg agtttcaaaa
    12601 ctcgggtata tgtggctgat accaaagaac gcagaaaagc ctgttgatgg tagaacaaaa
    12661 agaagaaagg aagtacaata tgaatagcgt tttgattata gatgatgaca aagaactttg
    12721 tgctcttatg aaaaaatgtg ttgagcaaga aaatttatct gctattactg cacattgtgg
    12781 tgcagaaggt ctacatttgg tcgataaaaa tagacacaca ctctctcttg taattttgga
    12841 cataatgatg ccagatatag acggttttca agttctgaaa aaaattagag aaacaagtaa
    12901 tattccagta attatgctta ctgctaaaaa tgacgaggat gataaagttt ccggactacg
    12961 tttaggtgct gacgattacc taacaaaacc gttcagcata aacgaactta tggctcgtgt
    13021 caattctctt attcgtcggt atactatgtt aaaccctgct tttataacag acacagattg
    13081 cataataata aatggcatga ttattgacaa gacaaaccga attgtttctg tcaataatat
    13141 tcctgttcaa cttacaagca aagaatttga tttacttgct tttctggctt ctaataaggg
    13201 acgggtgttt accaaaaaac agatttatac acaagtatgg gaagaagaat atgcttttga
    13261 cgatagtaac atcatgtctt tcatcagtaa attgagaaaa aagattgaac cagaccccaa
    13321 ccatccattt tatattttga cagttcgtgg tgtcggttat cgttttaata aggaggctta
    13381 acatgaatac aagtgtctat ctgcttttga tattggctct tagcctgatt attatagcgt
    13441 accttgtttc tgtcctgcta cgtgtccgaa tacaacttac acttataaaa gatgctttag
    13501 aggatataaa aaatggaaac cttaatcgac gtattttaac agatgaaaac gacattacaa
    13561 aacaaatttg ctatgatatt aacgaaatag caatcaatag ccaatttcaa cttattcagc
    13621 aaaaacaatc agaacaagcc tacaaacagc taatgacaag tctctcccat gatgtaaaaa
    13681 ctccacttgc ttctttggtc ggttatttgg aagcagtaga aagtaaaatt gttgtaggag
    13741 aggaaaaaga cgagtatatc catgttgcgt ctaataaggc tcattactta aagaattttg
    13801 tagaaaatct atttgaatgg gtaaaattgg attctaagga acaagttttt cattttgata
    13861 tttttgactt gaatgaatta tcccgtaata ttatatctga ttggatacct gttttagaaa
    13921 gcagtcattt tgaatatgaa tttgatatac cagaaataga gtacttctta cgtatagacg
    13981 caaatgcata tacccgtatc attaataacc tattacaaaa tattattacc catagtagtg
    14041 gcaacaaaat gacattgaga atttttgaaa acaaagagca agctcaaatt ataataacag
    14101 ataatgggaa aggtatatct tcagacaatc tcccacatat ttttgaaagg ttgtaccaat
    14161 gcgaccattc acgagcttca aagggaaatg gtttaggctt ggcgattgca aaggaactta
    14221 ttaacgctca taagggaaat attaccgcca acagcactcc cggtatggga acagaattta
    14281 caatattgct tcctaaagcc ctgtaaaagt acgaggcttt cttactctaa aacaagataa
    14341 aagcaagatt atggcaaggt tttggcaagg ttcatgtttt ataattctaa ttgtagttga
    14401 ttttgacgtt gtatcaacta tcttttgtag aaacaaattt agtaaaagtg aggaaattat
    14461 atatgaatga ttttgtgatt gaaacaaagc aacttacaaa aatttatggg aatcaaacag
    14521 tagttgacaa agtcaatctc catgtaaaaa aaggccgaat atatggtctg ttaggacgca
    14581 acggagctgg gaaaaccaca attatgaaaa tgattttagg attgacttca atcacgtatg
    14641 gcgaggtaga tgtatttgga gagaatatta agggacgtga aaaacaagta tatccccgta
    14701 tcggtgcaat tatcgaaacg ccaggatttt atcctaatct gacaggaacg gagaacttgg
    14761 agatttttgc aaaactgcgt ggtacagccg cccctaattc tgtaaaaaga gcattggaaa
    14821 ttgtaggctt accttataaa aataaaaagc tgtttagtaa atattcactt ggcatgaaac
    14881 agcgacttgg tattgccaac gctatcctgc atgacccaga actgttagtt ttagatgagc
    14941 caaccaatgg tcttgaccct attggtattg cggagatgag aaagtttatt agggacttga
    15001 gcgttgagca tggaaagaca attcttattt ccagtcatat tctttcagaa atctcattgt
    15061 tggctgatga tattggaatt atcgaccacg gtgttctttt ggaagaaagt agtatgagtg
    15121 aactagaaaa gaaaaacagt aaatatatcc tgctacaagt ttcagatatt ccgaagacat
    15181 cacttatttt agagcgtcaa tttaacacga aggattattc cgttcaagat gactacacgt
    15241 tacaccttta taacactaat ctggatatgg gtgaaatcaa taaagcactt gtaatgcaga
    15301 atgtaattgt tattagttct caactttgta atgatacttt agaggactac tttaaaaaaa
    15361 tcacaggagg agagggcatt gcttaaactt attcaaacag aatttcttaa actgcgtcgt
    15421 aggaaactta tttggttgat gttgttgtct gctctcatta tgccgttttt cgcactacta
    15481 tacttcaatt attttggtaa aacaggtgtt gagccaacaa agttttataa atggtctgca
    15541 tttggttata cactttggat tattttgccc gttgttttgg gtatattttg caccatgctt
    15601 atacacgaag aaaaccagta tgatatgctc aaacagcttt ggattgtacc tattagtaaa
    15661 atgggatact tttttagtaa atttttcgtt gttttgattt actctatttg ttttatgctg
    15721 attacagccg ttgcttctgt gttatttagt gtactttctg gatatgtcgt atttagctgg
    15781 gcaagcattt tatatttgtt gaaaaaatgt ttagaaattg gtgtaatcac tgcttttgta
    15841 atgttgccga ttttggcaat cgctacatct caaaaagggt acattctccc aatttgtatc
    15901 acattggtat atgccttttc cggttttgtt ataatgacaa taaatatgta tttacaccca
    15961 ttatccagta tgggagttat tatcatgaga aataaagaca ttccaggtct tatattttca
    16021 caggaaatta atgtcccatt agcatttctt tgtatttgta tttggagtat tgtatcagta
    16081 ttgtttgcaa acattgcact aggaagaaga aagtgaggtg tagataatgt ggaaattact
    16141 ttgggctgaa tgtcaaaaaa ttcgtcgttc aaaaattgtt tggattacag tttttgcatc
    16201 cattatgata gcagtcatag tttttgcagg agggcaggat gtatataatg acccagattt
    16261 acattatggc ttaaagagcg tttatgacgg ttcacggtat atagacaatg caggttggta
    16321 tatggacgca gtacaacctt ggtcaacttt ttttgttctt cctgctgtca ttgccctatt
    16381 aggaagctat attatttgtc gtgaagaaga ggaggacact attaaatccc tgcgtcttat
    16441 tcccataaat gaaacaaaat taaccatagc aaaaatgatt attactttta tattcagtat
    16501 acttctttat ttgttactct ttactataac atttttgact gaatccgttt tacatttttc
    16561 cgatttatcg acaaaactgg ttttaagttg catgaaagag tattttcttg atggcatagg
    16621 tgtattcttt gcaatttctc ccattattgc cttggtatcg cgcatgaaaa aaggatattg
    16681 gcttgcatta gtattcacag aagtttattc tattgctgga ctatttgcgg gtatgtcaaa
    16741 tgtcttacaa accttttttc ctattgcggc aatatttaat atttctggat actatataac
    16801 tactggagaa aaaattatgg ggagtatcat aagtttatta ctttgtgcct gcctttctgc
    16861 atttatactg aaagggctaa aacacaatga aaaaaattga aatatgtata tcaaaatgaa
    16921 aaagataaaa aaactgatta ttctaattgt attcttgttt ctttttggca tgttacactg
    16981 ttatactgaa tggaaattca acaatgaccc acctaaaggg gtaatagaaa gaataaatga
    17041 gtggactgat aagattggac tggattagta acttatgtta attatagtga atgtaatatt
    17101 agcaagttat tgtttcgtat tttaatacta taactttaaa tatttggttt taatcttcca
    17161 aacgaatata aaaaaactgt cacagaggca acatattttt gaatatttat aaagtggtag
    17221 ttttgatttt caaagctgcc gccttttctt ctatataagc aaacttatcc tcaacaaagt
    17281 gtaaaacatg gtacatttta agaaaaaagt accacagaca caagcaataa aagaaacgcc
    17341 gagaacctac aaaaacgtag tgcttatgca gatttacgac gccttctatt ttctcaaccg
    17401 tcttaatgac cttgtacaaa agaaattttt ctaatatttt ttataatctt atcttgtgat
    17461 gttttttatg tataaatgac ttaataacaa atccaataaa aattatggat attggtataa
    17521 ttagaacgag gaatttataa tcattataat gtgacattat tcttacccaa ctctcagaaa
    17581 aaatatatcc tatactgatt aatgaagtat tccatatggt tattcccacg atagatgata
    17641 ttacaaaagt tataatattt tgacctgcta tacctgctat aaaagatatg tatgttctac
    17701 ataaagggat taatctacat acacaaacag aaatagaaga gtatttatta aaataatctt
    17761 gagatgcaaa tattcctttc tttgcctttg gacatatctc tattatttta tttatgatag
    17821 atttacctcc ccaatttccc actgtataac aaaatatcga accaagtata cctgcaatta
    17881 tacttaaaat taaaattgtc agaaatccaa aatgatttct tgcagcaatt gctccacata
    17941 aaggtaaaac aacttcgcta ggtaatggaa aacaagcata ctcaatcata attaatacaa
    18001 atacagagat taatccatat tgtaaaataa aactattgat tacagacact tacttatcta
    18061 tctcctttct tatttaaagg ttttaagggt acatgttcta aggattctca ccctatattt
    18121 aaagaatcaa tctctttaaa ctattatatg tatttacaca aaacatttat aacatacttt
    18181 aaaaatagta ccttaaaata agaaacttaa taaataaaaa aagattacgt ggctacgtcc
    18241 tactctccca ggcag
//
LOCUS       DAGSHT010000437        10736 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.guided.115, whole genome shotgun sequence.
ACCESSION   DAGSHT010000437 DAGSHT010000000
VERSION     DAGSHT010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.guided.115"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            <1..297
                     /locus_tag="MJB72_003895"
     CDS             <1..297
                     /locus_tag="MJB72_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY8264923.1"
                     /translation="GIGAKDILEDGKIHDDYRIDYLRSHINACKLAIEDGVEMIGYCP
                     WSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIKKDSFYWYKEVIENNGIVK"
     gene            325..1173
                     /locus_tag="MJB72_003896"
     CDS             325..1173
                     /locus_tag="MJB72_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY8264924.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            1834..2526
                     /locus_tag="MJB72_003897"
     CDS             1834..2526
                     /locus_tag="MJB72_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY8264925.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLAELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            2523..3458
                     /locus_tag="MJB72_003898"
     CDS             2523..3458
                     /locus_tag="MJB72_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY8264926.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            3606..4529
                     /locus_tag="MJB72_003899"
     CDS             3606..4529
                     /locus_tag="MJB72_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264927.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            4513..5283
                     /locus_tag="MJB72_003900"
     CDS             4513..5283
                     /locus_tag="MJB72_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264928.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            5293..6075
                     /locus_tag="MJB72_003901"
     CDS             5293..6075
                     /locus_tag="MJB72_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264929.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            6451..6987
                     /locus_tag="MJB72_003902"
     CDS             6451..6987
                     /locus_tag="MJB72_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY8264930.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            7019..7408
                     /locus_tag="MJB72_003903"
     CDS             7019..7408
                     /locus_tag="MJB72_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY8264931.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            7445..7831
                     /locus_tag="MJB72_003904"
     CDS             7445..7831
                     /locus_tag="MJB72_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBY8264932.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            7958..9400
                     /locus_tag="MJB72_003905"
     CDS             7958..9400
                     /locus_tag="MJB72_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY8264933.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            9697..10512
                     /locus_tag="MJB72_003906"
     CDS             9697..10512
                     /locus_tag="MJB72_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY8264934.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTIELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCRDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         3837 a         1343 c         2016 g         3540 t
ORIGIN      
        1 ggtataggag ctaaagatat attagaagat ggaaaaattc atgatgacta tcgtatagac
       61 tatttaagaa gtcatataaa tgcatgtaaa ctagctattg aagatggtgt agagatgatt
      121 ggatattgtc catggtcatt tacagacctt ttaagttctt ctcaaggttt taataagcgt
      181 tatggacttg tttatataaa tagaactgac catgaagttt tagatttaaa aagaatcaag
      241 aaagatagtt tttactggta taaagaagtt atagagaaca atggaatagt aaaataaaca
      301 taaatatata ttgagggttt tagtatgtac attacaaagg tattaaataa ttcgttgcta
      361 cttgcaaagg atgataatga tgaagaaata atattgatgg ggaaaggcat tggccataat
      421 tataaggccg ggtctgagtt aaataaaaaa gatatagaaa agatttttgt tcttcatgat
      481 gaaaatataa aaaagagcat tatacaattg gcaacagaaa tagatgcaga atattttggt
      541 atagctcaaa tgataattgc atatggaata gaaaaatata acttaaaact tatgaaccat
      601 atatatttgg ccttaactga ccacattgcc tttgcagtga gacgttttaa agaaggtata
      661 atgattgaaa atcactattt gttcgaaatc aaggaattta atccaaaaga gtatgatata
      721 ggaaaatatg ctattactgt ttttaaggag gttcttggtt tagacctacc agaagaagag
      781 attggtaata tagcaggcca ttttataaat gcacaacaag ataatcctta tagtgataga
      841 aataagagaa gtgctaaaat tgtaaatgct attttgcaaa tagttcatta tcacttttct
      901 attgtatata ataaagagtc attttattat aggagatttg taatgcatct aaaggccttt
      961 tcacaaagat ttctggctaa tgaaccatca agagaaaaaa tagattttat atatgagcaa
     1021 gttcaaaaaa attgtaagga agagtatgaa tgtgtgaaaa aggtaggagc ttatatgtat
     1081 aaggaatact ccagagaatt accaagacaa gaggagttat atttgatgat tcatattcat
     1141 aagattttag gagaattatg cgaaaatagt tagaatatct taatatgcta gtaatattat
     1201 aaaaaattaa aaattaacta cactttaaat atgaataatt gcattactta agggggttgt
     1261 cttaaaattg atttttttaa ttaaatttga agctcaactc cttttttaaa cctttatatc
     1321 attacaactt taatgtttat acgcctgtac ttcagatgta tatctacttt ttacatttct
     1381 caacatgcaa atctaccatt ttttgcagta tgttaattga aataaaccat tgagttaacc
     1441 ctaaatttat tctggactta taggaatagt tccaaagata ctttaaaata ttttctggaa
     1501 taattgctta tttcacatat gttttattgt gatgttttga tttaataaat tcttcaactc
     1561 tgtatatagt aaatgtatta tcatgtttgt tttattcaag cacacttata atacgcttga
     1621 ttttttcaca atgatgaaat atactaatgg gtgttataag tataagaata gcattacact
     1681 tgaagaagca agtgataaat gaggtataaa tcctcatatg ataaactact atttcattga
     1741 ggagcatatt ctaaatgatg taaagatact acaatatgac taaattccta atgataaaaa
     1801 agtctacgaa tgagtgtaga aaggggaact aagatgaata aagttttaat tatagatgat
     1861 gataaagatc tttgtatttt gttaaaaaag agtatctcaa ttgaacagat tgaagcaaac
     1921 tattgccatt ctgggagaga tggactggct gaactttcaa aacaagatta tcagttagta
     1981 gttctagacg tgatgatgcc aggtatggat ggttttgaaa ctatggaaca aatacgaaaa
     2041 tatagcagta ttcccatctt aatgctgaca tcaaaaagcg ataattacag caaagtgcat
     2101 ggattgcgag ctggtgcaga tgactatcta accaaaccat ttgaaattac agaatttact
     2161 gctcgtgtac tgtctttaat ccgacgttac acccgtttta atccaaaaaa tgaggagcca
     2221 cagcaacttt ctttcaaagg tttgttgatt gaccttgacc agcatacagt gactaccaat
     2281 ggcgaaccta tgttgcttcc acccaaggaa tttggtgtgc ttctttactg tgcaaagcat
     2341 cagggtaaga ttttgacaaa acagcagatt tatgaagccg tgtggggtga gccatatgta
     2401 tatgatgaca gcaatatcat ggctattatc agtcgcctaa gaaaaaaaat tgaactagac
     2461 ccatcatctc ccatctattt gcaaacaata aaaggaattg ggtatcaatt caacacggag
     2521 gtatgaaaat ggacgaacaa acgataattc ttttcttact cttgctgtcg attggtctac
     2581 tttttctttc catatttgct ttttccaagc ttgggcatat ttacaagcgc ctaaaagata
     2641 tagaggagat actggctgat gtacaaattg gagacgaaaa tcgtaaaata ctaattaagc
     2701 catgtgatgt tatggctcca ttggtttatc agcttaatga gattgtctac gattatgaaa
     2761 ataagttact ttctctgaaa aagtcggata aagctagcaa acaacttatg acaagtttat
     2821 ctcatgatgt tcgtacacca ttaacaactc tgattggtta tctggatgct gtacacagtg
     2881 gtatagttat cgaacaagaa cgagaggaat accttgaaat cgctaggcgt agagcctacg
     2941 acctcaagga ttacattgat gttctttttg actggtttcg gttgaactct gatgaattta
     3001 cgctgtcaat agaatctgta gaaattgcag agttgtcacg caatattttg aaagactgga
     3061 ttcctatatt ccatgaaaag aaactgaatt ttgaaattga tattccagaa aatcgactaa
     3121 tggtaaacct cgacccagat ggatattccc gtgtggtcaa taatctggtt caaaatgttc
     3181 ttgctcacag taaggctcgg caaatcaaga ttaccatgag tgaggattcc agaatggtat
     3241 tactccgtgt tgaggatgat ggtgttggga ttgccagaga aaatttacca catatttttg
     3301 agcgactgta taagtgtgat aaaggacgtt cagaaaaagg aagtggtctt ggtctatcta
     3361 ttgtcagtca aatggtggag cgaatgggtg gtcagatttc cgtagaaagt gagattggaa
     3421 agtataccat atttacagtg agcttaccat taatataaaa ttgtttttcc cccatgtgac
     3481 atgtatacac agggggattt tttgtaagtt ataaagttgc aaggttaata caaggttgtt
     3541 gcaaggttaa tgtaagattg aggtggtatg ataactttaa taaacaaagg aggcttttat
     3601 tatttatgaa taatttaatt attgaaacaa aaaatctcac taagcaatat ggtgctcaaa
     3661 agagcgtttc taatttgaat attcacgttc agaaaggtcg catttacgga ttgctaggaa
     3721 gaaacggtgc gggtaagacg acaactatga aaatgttatt gggtctaaca aaaccaacta
     3781 ctggagaaat taaaatattt ggtcaggaca ttcacaataa cgagaagaag cttcttcctc
     3841 gaattggatg tttgattgaa tcaccagggt tttatcctaa tctgacagga acagaaaatc
     3901 tcaaaatctt tgcaaggctt agaggaatac caaaaaaaaa tgcagtagaa tcagcactgg
     3961 atgtagtcgg tttgccaaac aatgacaaaa agttattttc tcaatactct cttggtatga
     4021 aacagaggtt ggcgattgct cttgccatta tgcatgaccc acaactgttg attttggacg
     4081 agcctatcaa tgggcttgat cctattggta ttgcagaagt aagaacattt attcgtgatc
     4141 tctgcacaga gcgaggtaaa actattttga tttctagtca cattctttct gaaattgcct
     4201 tgttggcaga tgacattggc attattgacc atggtatttt gttggaggaa gaaagtcttg
     4261 cccaattaga agaaaaaaac agtaaataca tacattttgc tgtatctgat gcagcacagg
     4321 cagcaaggct tctggaaacc caacttaacc tgaaaaattt caaagtagca gataattaca
     4381 atattatgct atatgataca gatgtttctg ttgcagaaat aaatcgtgct ttcattctaa
     4441 acaacattga ggtatcagaa tctcatttat gcgaagatac tcttgaagat tacttcaaga
     4501 aagtaacagg gggtgaagga attgcttgat ataatttcat gtgaattttc aaaactcaag
     4561 cggttaaaat ttatactgat ttctatttta ggtgcttgct tatttccaat tcccgcaaca
     4621 attttgattg caaaggataa cttgcccttt gagcaattgt tcaaattagt tgtcaatttt
     4681 ggatattttt tgctattacc aattgtatta agtattgttg ctagccaact attttttata
     4741 gaaagagata atgatgtatt aaaaaatctt gcaacagtgc cagtgcaaaa aggaaaactg
     4801 gctctggcaa aattggctgt gttgctgttt atatcattgt tctactctgt tgcagggtta
     4861 ggagctacta tcattggagg tttcattgtt ggaatagtag agggggttgc tgtaaaatta
     4921 ggaatgagca ttgcttttgg aattatgttt tttgttactg tacttcctgt tgtggtactc
     4981 attgtatatt tcaatcgcag ctatattttt tctattatac tttcttttgt atatgctatt
     5041 ttcaatttta gtatatcctt aaatattatc aattttgaac caaataaccc aatattaagt
     5101 gtattacctg ctcctgttat aatgcgttgg tggatggcat tttggggaga tcccactggc
     5161 gaatacacgg cacttagaca gccatatttg ctctcaactc ctgcttgtgt aggtatttta
     5221 tgtttgataa ccattatagc agtcctgctg atttgtatga tatacaaaaa gcaggagaat
     5281 taggaggtat ctatgcttga tataattact acagaatttc aaaaaataaa aagatataat
     5341 attctatgga ttggtgttgt tgctgttctg ttttcagcac tattggcagt ttttcaggtt
     5401 gtaagttcac acggcagtga ccctctaacg tatgagagct ttgcaaacgg agttatctgg
     5461 aataatttca gtcttagttt tccatttggg attacactta ttggaggata tcttattaac
     5521 cgtgaatata cagaccaaac cttaaaaaac atcctaactg ttcctatttc attacgcaaa
     5581 ttgctagtgg gaaaattaat tgcggttgga ggaattactg tattgttaag tcttttcagc
     5641 ttcttatgca catttatttt ggggttgtta ttttgcaata ttaatatatc tgttacactc
     5701 gttgtaaaat ccttagttca gattttgtct gttaatctgt actgttatgt tgctgttttg
     5761 ccgattattg cttattttgg tcgtaaacaa aatgcatttc tgacgggtgt agggattgca
     5821 tttgtatacg gtttttgtgg tgtgtttgtt gctggaagaa atctgacaga cttttatcca
     5881 atcacagcag gtcttggtct agttggatac aacaatggtg ctggtactgt ttatcagcca
     5941 cttattggtg ctatgacttt gattgtaatt ctaatcttga caactattct acttgtattt
     6001 acacctaatt atgataaagt tatgaccata tctaagaaaa aagggataaa gcataaaaag
     6061 tattctcata aataacataa atattgtttc tattatttaa gttgctagac agtgaaaaga
     6121 aaaaatctgt cgtacagtgg ctatatgctc atgtattcaa gaagctacag ttttgatttt
     6181 tcataactgt agcttctttt tgtatttatt aaatcacgac tatcataata cttgcaatag
     6241 gtatagcgac aagagagagt actgtcatat gtgttttata tattacatta agtatattct
     6301 aaaatatatg ttttaaataa tatatttgaa aataggaata tttaaataaa aaaaataata
     6361 ttatatatag ttgacaaaaa ttggcaaatg atataatata taaatataga aatagtaatc
     6421 attactatta ttaaaaggga ggaattaatt atgaacttaa aaggaactaa aacagaaaag
     6481 aatttaatgg caggatttgc aggagagtca gaagcaagaa ataaatatac atactacgct
     6541 tcaaaagcaa gaaaagaagg ttataatcaa atagctgcta tatttgaaga aactgctaac
     6601 aatgaaaagg aacatgctaa attatggttt aaacttttac atgatggtat gccttcaaca
     6661 gaggaaaatt taaaggatgc agcagcaggt gaaaactatg aatggactga tatgtatgca
     6721 aaatttgcta aagaagctag agaagaagga tttgacaaga tagcttattt atttgaagct
     6781 gttggaaaaa tagagaaaga gcatgaagaa agatacttaa agcttttaga aaacttaaat
     6841 gaaggaaaaa tatttaaaag agatgaagaa gttgtttggc aatgtcaaaa ctgtggtcat
     6901 gtgtatgtag gtactgaagc accagaaaaa tgcccagtat gtgaccatcc aaaagcatat
     6961 tttaatataa aagctgaaaa ttattaaaaa ttatcattat taaaaaggaa tgttaactat
     7021 gaaattttct aaacaacgag aactgatttt aaatgaaata ttaaataatc cggttcatcc
     7081 tactgcggat tacctatatg aaaacttaaa aaaagataat ccaaatttaa gcttaggaac
     7141 tgtgtataga aatttagctc agctaacgga acatggcttt ataagaaagg ttagtattcc
     7201 aggatatcca gatagatttg atggtcgaat agataatcac tatcatatca tatgtgaagt
     7261 atgtggagag gtatacgatt tagaatccga agttcttaat aatttacaag aattaatatc
     7321 tgaggagaca gacataaaaa taacatctta taacataagt tttaaaggga tttgtaataa
     7381 ttgtaaaagg tgtagccaag taggttagtt ttcaaattct aaaaataatt aatgaggtga
     7441 gtttatgtgt agtgaacaaa aattttttat atgtgagact tgtggaaacc ttgtaggaat
     7501 gatacaaagt ggtggtgtac ctattttctg ttgtggaaaa cctatgaagg aacttgttcc
     7561 taatacaaca gatgcagcag ttgaaaaaca tgtacctgta attgaagtgg atggaaataa
     7621 tgttactgtt aaagtaagta gcacaactca tccaatgact aaagaacatc atatagcatg
     7681 ggtgtatctt atgactgaac aaggtggtca acgcaaatgt ctagcagtag atggagaacc
     7741 tgtagttaaa tttgctttga atgatgatga taaagtgatt tctgcctatg cttactgtaa
     7801 tttacatgga ttatggaagg ctgaactgta atatttaaat aaattgagtc aaaaaggagg
     7861 ctataaatgt attttcatac aaatagcctc ctataaattt atataaattc aagttttaat
     7921 tttgtttaat gtcaatataa tataaggaga aaataatatg tcaatttata aatgtagtgt
     7981 ttgtggatat atttatgatg aatctaagaa tgataaaaca tgggatgaat taagtgaaga
     8041 ttgggaatgc cctgtatgta caaaaggtcg tagttatttt ggaaagataa gtactgttta
     8101 ctatgaagaa gatgaaaaga tagcagaaga catagttgaa gatgaatcta aattaaatac
     8161 tgaaaaagag ggagatttaa attacttaag tacatattta agaagagatg atgaagttga
     8221 gaaacatatg gatataattc acgaaatggc tgttacagga aaatctataa tagaacctat
     8281 gagaactaaa ctaccagtaa tttcttggga tgatatttta attatgggtg ctcaattaaa
     8341 tccattacct ttaaatgagc atgatgaagt aaatacaaca acaataatag gaaaaaaagc
     8401 aaagaaacct atgattatag aaaatccagt ttatatttcc cacatgtcat ttggagcttt
     8461 gtctaaagaa ctaaaaatag ctttagcaaa gggtgcagcc caaaataaga cagctatgtg
     8521 tagtggtgaa ggaggtatat taccagaaga gaaggaagct tcatacaagt atatttttga
     8581 gtatgtacca aataaatata gtgtaactga agaaaatctt aaaaattctg atgctataga
     8641 gataaaaatt ggtcaaggaa caaaacctgg tatgggaggt catcttcctg gcgaaaaagt
     8701 aactgaagaa atagcaaaag taagaaataa gcctgtaggt caagatgtaa taagtccatc
     8761 ttgttttgag gaaatacaat ccaaagagga tttaaagaaa cttgtagatg aacttagaga
     8821 agtttcagaa gggcgtccaa taggtgttaa aatttcagca ggtcatattg aaaaagatat
     8881 ggaatttata gcttatgcta aacctgattt tgtaactatt gatggtcgtg gtggagctac
     8941 tggagcaagt ccaaagttgc ttaaagatgc aacttcaatt ccaacaatat ttgctcttta
     9001 tcgtgctaga aaatacatag atacacatgg attagatata gatttggtta taacaggtgg
     9061 gcttcgtata tctactgatt ttgctaaggc aattgctatg ggggctgatg cagttgctat
     9121 tgcaagttct gctcttatgg cagcggcgtg tcaacaatat cgtatatgtg gttctggtaa
     9181 gtgtcctgta ggagtagcta ctcaggatga ggaattgaga aaaagattac atatagaaaa
     9241 cagtgctaat agagtggcaa actttttaaa tgtaagttta gaagaactta agacttttgc
     9301 tagaatttct ggtcataaag atatacatga tttaagtgtt gatgatttat atactgttaa
     9361 ctctgaaata tcaaattata caaacataca gcatgtatag attgaagttt gaaactatta
     9421 gattttagat agctataaaa gtaaatgata aatatatttt ataaaagaga gtgtcttaaa
     9481 atgaactttt tagttcataa gtcactcttt tttttatgaa gccaaatata tttatcattt
     9541 tagcaatgaa aaagaatctt atctctttat tttgagatag attccttcta tttatgaaaa
     9601 ttaaagatat taaattgtaa aatattctgt gatgtcgtac tattaaacta aagattctgt
     9661 actttaaagt aaataaaaat ttaaggagtg attattatgg aaagattgaa gatacaagtt
     9721 ttagtcgaca ataatactta tattgataga tactttgttg gagaaccagc agtaagctat
     9781 tatattgaga ttgatggaaa taggattttg tttgactcag ggtattcaga tgtattcata
     9841 tcaaacgctg aaaaattaaa tatagattta ggaaatttaa ctcatgttgt tttttctcat
     9901 gggcataatg accatacaag aggtatccag tttttagaaa ataggtatga tttatctaca
     9961 attgaactta tttcacatcc taactgtttt atacctagaa aacatggaga gaaaagcata
    10021 ggagctccat tttctgctga ggaaattaaa aatattttta tgtataatcc taaggataag
    10081 ccatttaatc taagcaagaa ttgtgtgttt ttaggtgaaa taccttctat aaatgatttt
    10141 gaaaaaagag ctaaaattgg taaatgtaaa ataggagatt tgtgggaaga tgattatgtt
    10201 ttagatgatt ctgctatagt ttgtaaaact gataaaggta tttttattat tacaggatgt
    10261 tctcatagtg gaatttgtaa tatagtagag tatgctaaaa aggtatgtag agatgataga
    10321 gttattggta tactaggtgg ttttcatctt tttgaattga ataataggtt agatagtaca
    10381 atacaatatt tggataaaga agaaataaga atgttgtacc cttgtcactg tgtttctttt
    10441 aaggtaaaag cgaagatgaa tgaaatattg aatataaatg aagttggagt agggctgacc
    10501 ataagtatat agtatttgaa agtatataag atttaagttg aaggttaaaa agatattttc
    10561 tatagaaaat atcttttttg ttacgtttat tttaagagaa atcaatatac ttttatatga
    10621 aatattgtaa aaaaatttat ttttaatgag aatatatttt cattaataaa caattttatt
    10681 atgtattaat aatgttacaa taaatataaa taaatcaaag ttacttacat tacagt
//
LOCUS       DAGSHT010000438        10215 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.guided.121, whole genome shotgun sequence.
ACCESSION   DAGSHT010000438 DAGSHT010000000
VERSION     DAGSHT010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.guided.121"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            246..863
                     /locus_tag="MJB72_003907"
     CDS             246..863
                     /locus_tag="MJB72_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBY8264935.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="MJB72_003908"
     CDS             971..3502
                     /locus_tag="MJB72_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY8264936.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="MJB72_003909"
     CDS             3936..4637
                     /locus_tag="MJB72_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBY8264937.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5760
                     /locus_tag="MJB72_003910"
     CDS             4687..5760
                     /locus_tag="MJB72_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY8264938.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIV"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="MJB72_003911"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="MJB72_003911"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBY8264939.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPILHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="MJB72_003912"
     CDS             7127..7933
                     /locus_tag="MJB72_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY8264940.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="MJB72_003913"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="MJB72_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBY8264941.1"
                     /translation="MMRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         3885 a         1065 c         1841 g         3424 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg ggactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacta tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattacat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtt gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 attacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagataaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgtgt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt atgaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt acctttaagg ttattattcc gattgtatga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtatttc ctatattgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tattggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtggatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagaag aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgatgagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc ttcctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaaa ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacat
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaat atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatgattgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta gctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcaaa tgaattattg agcagagtag gaagaaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttatactgt gtaatttatt ttactctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agttataaaa aggtg
//
LOCUS       DAGSHT010000439         9057 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.guided.142, whole genome shotgun sequence.
ACCESSION   DAGSHT010000439 DAGSHT010000000
VERSION     DAGSHT010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.guided.142"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            complement(118..885)
                     /locus_tag="MJB72_003914"
     CDS             complement(118..885)
                     /locus_tag="MJB72_003914"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBY8264942.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(1396..1545)
                     /locus_tag="MJB72_003915"
     CDS             complement(1396..1545)
                     /locus_tag="MJB72_003915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264943.1"
                     /translation="MTRVYYLFTTFDGILIRLNANLCKVSSKKKMSILLVDTGYIDIY
                     QLMKR"
     gene            complement(1556..2371)
                     /locus_tag="MJB72_003916"
                     /pseudo
     CDS             complement(1556..2371)
                     /locus_tag="MJB72_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2463..3131
                     /locus_tag="MJB72_003917"
                     /pseudo
     CDS             2463..3131
                     /locus_tag="MJB72_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3258..3878
                     /locus_tag="MJB72_003918"
     CDS             3258..3878
                     /locus_tag="MJB72_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY8264944.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            4034..4816
                     /locus_tag="MJB72_003919"
     CDS             4034..4816
                     /locus_tag="MJB72_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBY8264945.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            4941..5867
                     /locus_tag="MJB72_003920"
     CDS             4941..5867
                     /locus_tag="MJB72_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY8264946.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            5881..6319
                     /locus_tag="MJB72_003921"
                     /pseudo
     CDS             5881..6319
                     /locus_tag="MJB72_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            6680..7087
                     /locus_tag="MJB72_003922"
     CDS             6680..7087
                     /locus_tag="MJB72_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY8264947.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            7089..7319
                     /locus_tag="MJB72_003923"
     CDS             7089..7319
                     /locus_tag="MJB72_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY8264948.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            7709..7912
                     /locus_tag="MJB72_003924"
                     /pseudo
     CDS             7709..7912
                     /locus_tag="MJB72_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            7990..8343
                     /locus_tag="MJB72_003925"
     CDS             7990..8343
                     /locus_tag="MJB72_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBY8264949.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            8415..9047
                     /locus_tag="MJB72_003926"
     CDS             8415..9047
                     /locus_tag="MJB72_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBY8264950.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         3204 a         1364 c         1452 g         3037 t
ORIGIN      
        1 aaatagttgc ttttcagata taattgccca atttaaattt tactatctga aaagtttttt
       61 atatttaatt tatatcttca aaaataaaat accttctaat atttctatat aattaattta
      121 attaatactc ttatgtaata gattaagtac tgaatttaac tctttaaatg atatttgtcc
      181 tggtgctgaa acactttttg cagctccaaa agtcaatgca gaaccaaata tttcaccaca
      241 caatctactt ataacaccca ttcctgacat tgacattgtt attattggtc tatctgcata
      301 aattttaaac atttcatttg tagcttcaag tagcactaac acatcttttt cattttgcgg
      361 cataacagct attttgggta aatctgcgcc caattcctgc attctacata aacgagatac
      421 aatctcttct ttttttggag ttttattaaa atcatgatta gatataatta ctttcacttc
      481 ttttttatga gcaaaattaa ctacttcatc tatgacctca tctcccataa ataactctac
      541 atctattaaa tctactaaac ctgtattaga aatttcttta ttcaaagtag tataataatc
      601 tcttgatatt aatttttctc ccccttctac tacacttcta aatgtgaata ataaagggat
      661 atcatgtata taacttctta attcatagag aacttcctta acttctttaa tattttctac
      721 attctcaaaa aaatcaactc gccactctat aatatccaaa catgcatctt ttaactcttt
      781 agcctctttt ataatatctt ttttattttt cccaataatt ggtacacaaa ttttaggtct
      841 accttctcca atagtaatat tttttacttg aacttttctt ttcatattct cctccataat
      901 cagaaattct ataggacttt aaattctatt ttctgcacaa tttaattata cattaaaaat
      961 actatacatt ataaaaatac taatttaaaa ataattttta ttctaattct taaaacaatc
     1021 ccacatattt tagaaaaggt actctataat atacaaacac ttttttttac ctaaatttat
     1081 tttaacattc tataattttc acaatattct ttaaatttta ctcatataaa caatattatt
     1141 ctggatgcta atagaattaa tctataaatc atagtataat tatgcagaaa taactcgaat
     1201 ttcactactt atgaagagat tgaatattaa tgaatctaaa tcatttgcat tatttaagag
     1261 ttttgactaa agttgaacac catacaaaag tagctactca attgtttata ccccaaccaa
     1321 gcttaagtaa tgttatatag attctctaaa aagaatttag tatcttttta tttgaaaaac
     1381 ttatattaaa tttgattatc ttttcataag ttggtaaatg tcgatatatc ctgtatctac
     1441 aaggagtatc gacattttct ttttggaaga tactttgcat aagttggcat ttaaccgtat
     1501 aagaatgcca tcaaaagtag taaataagta gtaaactcta gtcatactac taggattata
     1561 accgttccat ttcagctctc gcagaactaa atgaaccatg tgcataatag ccaagcgtca
     1621 tagtaatgtt tttatgcccc ataatatatt gcaatatatt agtactcatt cctttgttag
     1681 ccatatttgt acagagtgta tgcctaaagg tatgtggcgt aatctttggt agcttgtcct
     1741 catgggtctt gttatacttc ttgactaacg ctcttaacat gctttgataa tgagttgctt
     1801 gttttggtaa tccatcttgt ttaagaaata ggaagttctg gtaaccatca acaatgattg
     1861 gttctgcttt agcgttctta cgccgttcaa cgatacgttc aaatgattga taagctcttt
     1921 cagtcattgg aatttctcgc ttaccctgtg tggtctttgg aacttctaca taatagccaa
     1981 tcttagtatc actcaacaat tgatggtcta ccataatggc tttatttttc atatctaatt
     2041 ctaaagtcaa accgcataat tcagaaatgc gtagtcctgt ttctaagagg ataacaacat
     2101 catcataata tttataataa gtcttatcgc tccccataaa ggataataag cccatttcct
     2161 gttctggtgt tagagcgatt tttggttctg ttgtatcttc caaaacatca cttaactaaa
     2221 aattaaatgg attctttcta atacagtcgt cttgaattgc catgaaaaac gacgctttca
     2281 gtgagcgctt ataattgtca atggatttat aagcacaccc atcttcactc attcggatag
     2341 cccagctttt agcgtctgat aatttaacca tatcaatgct ttttgcacct agtgggtcat
     2401 tgatagtatc tatattttga tttataattt atacattttt ttaaaaacag acttttaact
     2461 gtaaagaatc tatgtataaa cttagctatg ttgtccgtgt atctacaaag gatcttgacg
     2521 agctaaaaag gcgttgtgat gaagtcaaag acttttacga cgacttatca atcaagctcg
     2581 tgcgtccctt tggggatatg ttgggattgc atggcgaatt tattcctgtc agcaaacgtt
     2641 acatcaatga ttatattcaa tatgtcactt cggatttttt ggctggactt ggttttggag
     2701 cgacacaaac ccttggcgaa catgaggata tctatcttgg ctacaatctg gacactggaa
     2761 agaatgtcta tctaaaacct gcccttgcta gtcaaggggt aaagggttct attaccaatg
     2821 cgttagcgtc tgcgtttatc ggttcattgg gtggtggtaa attattcagt aacaatatgc
     2881 ttgtttacta tgcggtacta ttcggcggac aagcggtcat agttgaccct aaagcaaaac
     2941 gcggcaaatg gaaagaaacg ctaccagaaa tagcccatga gataaacatt gtcaatttaa
     3001 ccagtgatga tgaaaacaaa ggattgcttg accattacgt cattttgagc cgtaaaaagg
     3061 attctgaaag tttagcgatt gatatcctaa cattccttac tggtatctcc agtcgtgaca
     3121 gtgataagtt tttgcagaaa aatgacaact ggaaaataat aatggaatag tttctatgta
     3181 aatatatttt tactatctta cctaactaat tgaaagatga tataatttaa tcaaagattt
     3241 ggtaaggaga gatgaatatg catttaattg attttatgaa tgaagatatt aaaaagaagc
     3301 ttgttaaaaa aacgtataaa aagaaagaga ctattctttt tgctgagcaa gaaaatgaat
     3361 atgtaattct tatgattgac ggtattgcag aagcattcat cttaaatgtg aaaggaaaca
     3421 tctcaactat tcatctttat agaagtggta gtttttttgg agaaatggaa caatttaatg
     3481 atgggaaaaa acccgttgaa attgtcgctt tcacagattg taatgtatat aaacttcata
     3541 gagatgattt tttaaactgg ttaaggggag attttgaagc tacaaaattt ctgattcgag
     3601 aaatatctag taaattagtt gtaaatgctg aattaattga agaattatca tctttaagtg
     3661 ttaaagaacg acttctgagg tgtatatcct tacactatta tagaagtgaa ttaaatctgc
     3721 ttaccaaaaa acagcttgct agcgaagtaa acgctccgat tagaagcatt aatcgtgcta
     3781 taaatgaatg tgtacaagaa catctaattg cttatgaaaa caaaagattc agtgtaatta
     3841 atcagcgaga ggtattaaag aatcttccta caacataagt ataatttcaa aaaagtattg
     3901 gataataatt attcaatact tttttatttc gggtcaagtg acctattttt ttttatttct
     3961 tcattatata atactcctat gcataattat gttggattga tagctaataa aagacagtga
     4021 gaggtgttat attatgacta aaacacaaca ggaaatgata caaaacgtat tagaattttc
     4081 aaaaagagat agtaggataa taggacttgc aattagcggt tcttatatca caaaatcgtt
     4141 agatgaatat tctgatttgg attttctgat tgtagcagat gataaatcct atgaagaagt
     4201 tatcgaagag agattactta ttgtggaaca gtttggaaca ttagtttctg cttttactgg
     4261 cgaacatgtt ggagaacctc gattgataat ttccctctat gattcccctg ttcttcatgt
     4321 tgattttaag tttacaacct tagatggttt actagatagg gttgaagatt cagctattct
     4381 ctatgaagaa aacaactgca taacagaaat ttattctaaa aaagaagcat acttcccaac
     4441 tccagactta cagtggattg aagatagatt ttggatttgg gttcattaca cttgcactaa
     4501 gattggacgt agagaactat ttgaaacaat tgatttttta tcttttttaa gacaatcagt
     4561 acttgcctcg ttaatacagc ttctcttagg taaattacct cgtggagtta gaaaaattga
     4621 attggacgca ccccaatata ttgaagcatt agaaaagacg gttgctaacc atgaatataa
     4681 aagctgtgtt aatgcattgc aggctactat caacctgtat cttgaattaa gagaggaatt
     4741 atcacaaagt cctattgtcc gaaaggttga agctgaaagg gttgcattac agtattttca
     4801 agatttaatc aaataaaccc aaagtaaaaa aatattgaat agtaaatatt cagtgttttt
     4861 tcatttttgg tcaaatgacc tagattagtt gttgtaacaa ttctataatt attttgaaag
     4921 taacagtatg gaggataact atgagtgata ttttaagtag aattacaaat aaaattgggg
     4981 ataagaatat agttgataag ttatcagctc tatcaaaatc agatttgaac tcacttctat
     5041 tagaagtgtt tgatagacaa gcaaatactt taactgcaac tgatattcta aagtcatacc
     5101 aactaaatag atttactatc cctagcggta tagatccaga agaaattcat gctttggagt
     5161 caaaattatt gagaaaagca ttcaatatgg atatcaagac gattatgctt tctccctctg
     5221 ctccattagg tagctgtagc gtttttggct ccgtagatca atataatgta gtaagtgctc
     5281 tacgagggac agaaacactt gcagacccct caaatatgtt ggcaataatt attgctgata
     5341 aattaaaaag aaaagaagaa aataatacca tacctatcca catggctact actgctaggg
     5401 taataagagc acaaaatttt gtaggtaaag gtttatattc acattttggc ttatattgta
     5461 tggtttcatc tggtatagat acagggtctt acacttgtga aaaaatgttg ttagaaaagc
     5521 acttaaacta ttataaagat atattaagtg aaatagaaaa tgttcgtttc tctattattc
     5581 tacgcaaaag aaacggatac aaagataatg acggattttt cgacagaatg gttgaaacta
     5641 tcaaacttat ttttcctaat atagagttat caattcataa tgatgatgtt gataataatt
     5701 attataaagg tatcaatttc aaattatcgg tcaaacaagg aaatgaaacc gttgaaatag
     5761 ttgacggtgg atttgttgac tggacttatc aaatgcttgg aaataaaaaa gaacgctgtt
     5821 tgataagtgg tattggctta gaaagatttc ttgtagctgt ttcataaaag gagaagaaaa
     5881 atggaactta tagagtggaa ttatgcttat attaccgatt tagttgagtt tgcgaatgat
     5941 aaagaaattg caaacaatct tcgtgacaac ttcccccacc cttataggta gcaaaacgct
     6001 agagatttta ttagtttttg tttgacaact tcagatgtta aacaacttag ctatgctatt
     6061 ttttatcaag aaagggctat tggaagtatc agcctaacat ttgaagaaga tacctctatt
     6121 aataaaaatg ctgaattagg ttactgactt gctaggaact attggggcaa agggatagca
     6181 acaaatgcag taaaagaaat atgcaaaatt gcctttgaaa aatacaacct taacaaaatt
     6241 catgctaatg tatattccta caatagagag tcaagtaaaa tgtggcttta agattgaaga
     6301 atactgtcac aattcataat aaagaataga aacatgattg attgccatgt atatagttta
     6361 attcgaccgc aatagcattt attagaatta acctactttt taataacaga ataagttttt
     6421 cccctctatt aatttcttat gcatctttgt atttttatga aaagctagcc aatgcatatt
     6481 tccatacatc aatatggtgg tggcttacaa ccaatataaa ttaaatacgt tttaaagaat
     6541 acacattttt gccctatagg cagagtgtgt atttttttct cttttttttt gaaatttatc
     6601 gaagatttaa cgccctccca ccgtgatagg agtaagcaag gttatttaac tccttgcgat
     6661 ggaaagaggg tgaaatctta tggttgatga aaagacaatt gaacatcaat tcgacagttt
     6721 ctgtaaaaca gttttgcgta actatgctag agatatttat gacgaaaata aacgcagaaa
     6781 tgattatttg gtatctctcg aatctttgtc acttgcagaa ttaagtaaat tgagtatttt
     6841 agatgattat gattctaact acatctgtat ggtttcttac gactacaaca tacgaattga
     6901 agatgtatta atggcacaag caatcggaaa acttacaaaa agaaaacaag atataattct
     6961 actttcattc tttctcaaca tgacaaatgc tgacatagca acattgatgg atttagcaga
     7021 aaataccata cattatcaca aaacaaatgc actaaaagag ttgaaagaac tcatgcagga
     7081 acattgaaat gaaacctact aaagaagtat taccttttac aattatttta cttgctgtac
     7141 atggcgatgt aaatgcaata aacgtaatac ttaaacattt tgaacactat atcattcgtc
     7201 tttcacagaa aactttattt gatgaatacg gaaatcccta tattcatgta gaagaagaaa
     7261 caaagcgaat gctaggaaca aaactaatca ctgctatttt gagatttaat ttgtcttaat
     7321 ctcgccctct ctccatgtat caagctattt ggtacatgga gaatcactaa ttatttgtct
     7381 ataaaagccc ataagctatt gtgatttttt ataggcaaat caatgccgac tgttctttga
     7441 caactgaata aagtagatag atacgtttgt gatacagata gccaacaaat actatacgcc
     7501 atgaccttaa tcaagagagc tactatatag tgatttgtga ataacctttg gaacggttgt
     7561 tttgccgtaa cctgtaccac agtataatga tacacctgta tgacacggtt ctgcccataa
     7621 gaatgggatt ggttgaaata ccaatggagc ttaccaaagc catctatcta cttctgaact
     7681 acgaacatag aaaggagtga tacattctat gaacaatact gatgtaccta tttgggaaaa
     7741 atataccttg acgattgaat aagcgtcaaa atactttcgt attggagaga aaaaattacg
     7801 taaacttgct gatgaaaacc ttgattctgg ctgggtcatt atgaatagca atcgcattca
     7861 aataaagaga aaacaatttg agaaaatcgt tgatacactt gacgttatct aatgaaattg
     7921 agccttatat gttataatat tcatatgacg tatcaagctc aattcggtta ttgagaggag
     7981 cttcttatca tgtcaactga aaaaagacga gataataaag atagaatttt aagaactgga
     8041 gagagccaga gaaaagatgg aagatacgca tataagtatg tatattcatt tggtaaagca
     8101 caatttgttt actcttggaa acttgtacct actgataaac ccccaaaggg aaaacgtgat
     8161 gatatatcat taagggataa ggaaaaagca atacaaaatg accttgatga tagcattgac
     8221 actattggta agaaaatgac agtttgtcag ttatacgcta aaaagaatgt actaagaaag
     8281 aatatccgac ttaacactaa aaaaggacgc cattatctta tgaagattct taacaaggag
     8341 tgattctgtg aagagatgta ttgtagtagt ggaatagtca aaaacataat atatattaaa
     8401 ggagagattt aaatatgact attccaagtt caaataaggt atatccaaga agtaatgatt
     8461 atcaaactgt atatcttaaa aatgtaataa caagggaaaa tatagaagtt ggagattaca
     8521 caatatataa tgacttttat aatgacccaa gagattttga gaaaaacaat gtattgtatc
     8581 attaccctat aaacaatgat aaattaataa ttggtaagtt ttgctcaata gcatgtaatg
     8641 caaaatttct aatgactagt ggaaatcact caatgaaatc aaaatccaca tatacttttc
     8701 ccatctttta tgaagaatgg aaactcgata tcaaccatat aacggacgct tgggacaata
     8761 agggtaacat tataattgga aatgatgtgt ggataggtta tgattctata attatgtcag
     8821 gtgtaaatat tggtgatggt gcaattattg gaacaagagc agtagttact aatgacgttc
     8881 caccgtattc tattgttgga ggagtacctg caaaaattat aaaaaagaga tttaataatg
     8941 atacaatttc aaaattatta gaaattaaat ggtgggattg gccatataaa aaaattcaat
     9001 caaacattaa gcatattcaa tcaggaaata ttgataaatt agtttaatat tttataa
//
LOCUS       DAGSHT010000440         7762 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT600 isolate 16.7530621
            SAMN25517030-rid13962163.guided.162, whole genome shotgun sequence.
ACCESSION   DAGSHT010000440 DAGSHT010000000
VERSION     DAGSHT010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25517030
            Sequence Read Archive: SRR17631484
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250565.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250565.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 02:19:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,933
            CDSs (total)                      :: 3,837
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 25 of 63
            Pseudo Genes (internal stop)      :: 24 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25517030-rid13962163.guided.162"
                     /strain="RT600"
                     /isolate="16.7530621"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2016-05-17"
                     /collected_by="ModMedMicOxford"
     gene            210..674
                     /locus_tag="MJB72_003927"
     CDS             210..674
                     /locus_tag="MJB72_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY8264951.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            908..1270
                     /locus_tag="MJB72_003928"
     CDS             908..1270
                     /locus_tag="MJB72_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY8264952.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            1865..2701
                     /locus_tag="MJB72_003929"
     CDS             1865..2701
                     /locus_tag="MJB72_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY8264953.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLAFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            2785..4899
                     /locus_tag="MJB72_003930"
     CDS             2785..4899
                     /locus_tag="MJB72_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBY8264954.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            5440..6393
                     /gene="blaCDD"
                     /locus_tag="MJB72_003931"
     CDS             5440..6393
                     /gene="blaCDD"
                     /locus_tag="MJB72_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBY8264955.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            6737..7420
                     /locus_tag="MJB72_003932"
     CDS             6737..7420
                     /locus_tag="MJB72_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY8264956.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            7417..>7762
                     /locus_tag="MJB72_003933"
     CDS             7417..>7762
                     /locus_tag="MJB72_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY8264957.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSM"
BASE COUNT         3086 a          841 c         1285 g         2550 t
ORIGIN      
        1 ctaaaaatta ggcaagtctt ttaagtatgt tctgattata agagcactgt gtgacaagaa
       61 tgatgagatt atttttgcta tactttcgat ataagaaatg gaaggaggtt atgttttata
      121 ttcatatgaa ataattagtt acacagtaaa ttttcgtata atttggtata aaaagattag
      181 aaatgaaaat aaagtaatgg aggaataaaa tgactagaat atccaatata acaatagctg
      241 aacaatctga gtactgtttt ttggcaatac gaaaaacaat tgactttatg gtagagtttt
      301 cagagttttc aaaacaaagt tttgaaaaaa tttctaaata tctggaggag agaggaattt
      361 tatcttcagg agcaccgatt gtatgttttc ataacatgga tttagcaaaa ttggatgttg
      421 aagtagggtt tcctgtagcc acttatatag atgggaaaaa tgagattcta caaaggattg
      481 ttcctgcaca gaaaataata actgcaattg atttaggacc ttatgaatta caagacccta
      541 ctttagaaga cttattttca tgggcaaata gcaatggata taaacttcat ggcgatatat
      601 attaccagta tttaaatgat atcaatcgtc cagctagtga atatcttacg aaaatgatgt
      661 tacccattat atagaaagtt aaagattatc gtttgttagt tgataattag aagaacattt
      721 actgctacta cataaatttt atcctaaata atatcaaggt gatttaattt atgtattcta
      781 atgtaacccc aatatattgt taaggtctac ttaagatgct caaaaggcta tgcttaattt
      841 attgttatat aatttgtaga gtggtataat ttagaggtaa tattatatag atatataggg
      901 ggaaaatatg aaaatatatg atatattaac acgtttttat attcaagata tagaaaaagc
      961 catacctttt tatgaaaatc ttttaaaaga aaaatgttct ttgcgttttt cctataaaga
     1021 agttgggttg gaattagcgc aaataggaaa tgttttacta ttatctggtt ctgatgatgc
     1081 tcttaaacca tttatagaaa caaaatctac ttttatggtt gattctgttg atgaatggag
     1141 aagttatcta ttggataatg gagcagttgt agtaagagat aagaaaaaag ttcctacagg
     1201 atataatatg acattgagac atccagatgg tacaattata gaatatgttc aacatacaaa
     1261 gcaggattag ttaaaaacag attttactac tgagcaacta attagaatct attcttagac
     1321 gtacaattta ttatttttgg tattaacaga ttataagtca aattataata tttcaaaata
     1381 gaatagaaat ttatttacat aaaagagagt gtttaaaaat gaacttttta gttcatgaag
     1441 cactcttttt tgtatgaaat caaatacatc aatatataaa ataaactgat gaagagctta
     1501 aattaaaata aaaagattca taacaggacc ccattaaact ttaagatagg gtaagtttgt
     1561 aatcatatta aataattgta aaaagctgtt tttagtcgta taatataaat atttatcatt
     1621 taattaagga atataaaagg aatctaataa gaagaaaata ataaataact ttttagattt
     1681 actaaatgaa aaattattga aaattaaagg attagagtct ttaatcagaa agtaaaaagt
     1741 aatatattaa atttatgaat tgaaaactta aacaacaaat ttgtttataa aatagcaaga
     1801 ttcattaaat ataactaagt aataattgat ataatgttgc atggataaaa ggagggaagc
     1861 tgcaatgtct tacaaagtaa ttaatgtcat aactgctatt gactatatag aggagcattt
     1921 atcagaaaag ctggatttag atatagttgc taatgctgtt cattactcaa aatatcacct
     1981 gcatagaact ttcacaactt ctgttggcct aacaatgcat gattacataa agcggagaaa
     2041 gttgacagaa gcagcaaaat tattggcatt ttcaaagaaa ccaattattg aaattgcatt
     2101 gatagctgga tatgaaagtc aacaggcatt cacttctata tttaaagcta tgtataaaaa
     2161 atctccaaat aaatatagga aagaacaaga attttatcct ttgcagctaa gatttgtttt
     2221 gaaaaaagat aacctatttt caaagaatgt actagagttg gaaaaagaaa ttaaacttgc
     2281 aagtatgtct gacataccat tatggatgaa tttagtgaga ttagtcatag atggatttcc
     2341 aaatttacaa gaggaagaat atatatatca attagaacag tatattttag aagaaagagc
     2401 tttaatatta aaacttaata atgtcgctat tgcaaatatg gtgtttaata gagaaactag
     2461 aagtattgat ttttttggaa tacatccaca gtatcgtaat tcagacattg cacaagtgtt
     2521 tttgaagaaa gttattgaag atttcttgat agatacagat atttcaatta ctacctttcg
     2581 agagggtgac aaagctgata caggacatag agatatgata aagaagttgg gctttgcaga
     2641 agcagagttg cttgtggaat ttggatatcc tactcaaaaa tttattcttc ctttaagtta
     2701 aatagcaaga atgattaaaa actccaaaac acaattaagt ataatgattc agtgtaagta
     2761 cttaattaac aaaggaggat ttaaatgtct gaaaataaac aagtagtaaa ttgcgatatt
     2821 attgcatcta attctaaaca taatatgtgt gaatatgatg cagagttatt aacttttttt
     2881 ccagatgaga ttgcacatct aatagagata aataacaagc tagacaatgc ttttaagaaa
     2941 gctgaaaatc ttgttgacaa gcttgataag gattatatgg acgctaaaat gtatatggta
     3001 aaaaatcgtg gtgaaataga cccacatgaa atgtttcaaa atgaacaagg actaaaacaa
     3061 atagataact atggggcttt tatggtaaaa gttcgagata agattaataa aatcaaagat
     3121 tctccatact ttgctagaat agattttagg ctgaaagata tggatgatga atctaagtat
     3181 tatattggta gatttgcatt tgattatgaa gatgagctta taatacttga ctggcgttct
     3241 ccaattgcca gtatgtttta tgattatgaa atcggaaaag ctggatatga tgctccaata
     3301 ggatgggtgg atggagaaat aacaagaaaa cgacaattta aaattaaaaa tggaaaatta
     3361 gaatatgcac tagaaagttc cattaatata caagatgata ttcttcaaaa agaacttagt
     3421 cacacttcag atgaaaaaat gaagtcaatc atttcaacaa tacaaaagga acaaaatcaa
     3481 attattagaa atgataaagc tgatacactg attattcaag gggtagctgg ttctgggaaa
     3541 acatctatag cattacacag aattgctttt cttctttatc gatttaaaga taaaatatca
     3601 gcaaataatg taattatcct atcacctaat aaagtctttg gagactatat ttccaatgtc
     3661 cttcctgagc ttggagaaga gccattatgt gaattaagtt ttgaaaatat agcagaggta
     3721 caattagata gagttattaa ttttgaaagt gagaaagacc cattagaaat aaatgatgct
     3781 aaatggtctg aaagagtgcg ttttaaatct acacttgatt ttgtaaaact tattgatgat
     3841 tatataaaac aaatgccaaa taagattttt attccaaaag actacacttt tggaagtttt
     3901 acagctaaaa gtgactggat acagagtaga tttgaggcat ataacagata tccagttaag
     3961 aagagattag agaaggtagc tgaagacatt cattataaat ttgaatcaga taatattatg
     4021 gaagaagatt taccaaaggt aaaaagtata cttaaaagtt tgaatggaat gttgacaata
     4081 aaaaatactc ttacactata taaggatttc tttaaacaaa tgaatatatc aaacatgttt
     4141 gtgatggcaa caaaaaaaac tcttgaatgg tcagatgtat acccatttat atatattcat
     4201 gctgcatatg aaggtataca agaagataag attataagac atgttgttat tgatgaaatg
     4261 caggattaca cacctattca atatgctgta ataaacttac tttttaagtg taaaaagact
     4321 attcttggag attttggaca attggttaat cctaatcaca ctcatacact agatgatatg
     4381 aaacagctat ataatgatgg ggaactagtt atgttgaata agagttaccg ttctactttt
     4441 gagattatta atttcgccaa aaagattcaa gacatatctt cattagagcc tattgaacga
     4501 catggtgaag aaccagcttt attaaaatgt aataacgagc aagatgaaat aaataagata
     4561 aaaatagaaa ttgaagaatt taaaaagagt gataatgcga ctcttggtat tatattaaag
     4621 acagataatg acgctaaagc tgtatatgat gcgttaagag aaaaatatag tgttcactta
     4681 atatcttctg aaagttctag ctttacaaaa ggagtatcca taacttctat taaaatgtca
     4741 aaaggtttag agtttgatga agttattgta ccttcagtaa ataataaaac atattatagt
     4801 gattatgacc gcagtttgtt atatattgct tgtacacgag ctatgcataa acttaagttg
     4861 acatatactg gtgaattgac tcaacttata gatatgtaga ttatatactt attatgtaag
     4921 tgtttactta tataaatata acctgtcaat aaaattcatt aataaatcaa taaaaacaat
     4981 atttgtaaaa gtaaaagcta taaattatat attaaatttt ataatttata gcttttataa
     5041 tactgcttat cttaatcctc ccaatagcaa aataatttta aacatgccat attttataaa
     5101 tgtaataaca attatacaat taaaataact ttcccaaact aacttgacat ttaaaactta
     5161 ctattgtaat atttaaatat tacaatggta agttttaaat gtggaatcaa ttattatatc
     5221 acaaattaaa aggaggacag gatgttatta atagttactt aattatcttc gcaataggtt
     5281 taatcaacat aattgtacta ggtgaagaaa atataagaga tacagctatt catttagtct
     5341 tgtttcttat aagtagaacc tagattggag tatattacaa ataatgtcta ctattttaat
     5401 agtaggttat aaatttaaat atgggagatg atataaatta tgaaaagaaa aaagaatttt
     5461 atttggatag ctatcttatt ggttggtgta gttatggtaa tgtattatgt agggaaaaaa
     5521 cataacgata taaatcaaaa aactgataag aattatataa aatctgaatt aaataagtct
     5581 gaattaaata aatccaaaat tgaaaacaat aataaaaaca aaaaagaaaa aattaatatg
     5641 gttgactata gtgattgttt tgaaggtatt agtggaggag ctatttttta taatactaaa
     5701 aataaagaat ataatatata taacaaagaa ttaattgaaa caagaaggtc accatgttct
     5761 acttttaaaa tagtttcaac tttaattggt ttagaaaaag gggtaataaa ctcaaaagaa
     5821 tcagttatgg gatatgatgg tacagagtat ccaaataaaa attggaataa aaatcttagt
     5881 ttagaagagg catttaagga gtcttgtgtt tggtactaca aaaaattgat agataaagtt
     5941 gatgctaaat ctgtccaaaa cattttagat gatttaaagt atgggaattg cgatattagc
     6001 gaatgggaag gagatttaaa aaatggaaag ggacatttaa atggattttg gttagaatct
     6061 tcattacaaa tatctccaaa agaacaagtg caaacaatgg caaaaatatt tgagggagat
     6121 acaaacttta aaaaagagca tattaatatt ttaagagata ttatgaaaat agatgtaaat
     6181 gataaaaaca taaatgtata tggaaaaact ggaacaggat ttgatgaaaa aaataaacgt
     6241 gtggatgcat ggtttgttgg tatgctagag cgtgaaggtg acacctatta ttttgctatt
     6301 aaatcagatg attccaataa agaaattaca gggccaaagg taaaggaaat tgcaattaat
     6361 ataattaaga aatattatag tgtaagggaa taaacttaat tttatttgta aatattattt
     6421 acctatagtc tgtaatattt attaaataat tttactaaat tgagtctata tagagcaaaa
     6481 ctcctttagt acataaaata cttgctattg attattaaat tgaataccta tagatttaaa
     6541 gttaatacct tttaaagact atgtaatata ttaaaagtat aaataaaata tattgcatag
     6601 tttttgtttt atattataat tttaaataat gtgcaaaaac taaacaatat ttttaatagt
     6661 aatttatatt tgtattcatg tcactataat gtcattttag gttgctaaaa tttaaccata
     6721 aaggagatga ttgtatatgg aaatattaaa atgtgaaaat ctcacaaaaa tatatggttc
     6781 aaatcaaacc agagtgacag cccttaataa tgtaaattta tcagttcaaa aaggtgattt
     6841 tgtatccatt gtaggagcat ctggttctgg caaatctaca ttgttacatc tgcttggggg
     6901 agtagataga cctacatctg gaaagattta tgttgaagat acagaaatat caagtttaaa
     6961 ggaagaagct cttgcagttt ttagacgtag aaaagtaggt cttatttatc aattttataa
     7021 tcttatacct acccttgatg ttagaaaaaa tatattatta cccatgttac ttgataaaag
     7081 aaaggtggat gaggatagat tctctgagat tgtatctata ttgggattat ctgatagatt
     7141 aaaccatctt ccaagtcaac tttcaggtgg acaacaacaa agggtatcta tagctagaag
     7201 tttaatctat agaccagcta ttttattagc agatgagcca actggtaatc ttgatagaaa
     7261 aaattcagaa gaaattgtag acttgttaaa cttatcaaat aaacgattta atcagacaat
     7321 acttcttatt actcatgatg agaaaatagc actagaggca aatcgtattg taactatgga
     7381 agatggagtg attgtctctg aaaaggtggt gaagaaatga atattcttca agaatacacc
     7441 ttagactttg tacgacgcaa taaacgtagt agtatagcta taatgttagc tatattactt
     7501 actacaacca tgatgtcttc tttgtgtgga ctgttgtata ccatgtggac agattttata
     7561 aaactttcaa ttgagaaaga aggtaactgg catggtgaat tgtttgacta tacttatgga
     7621 agtgacttac cattaattga aaatttttct tcagttgatg atgtaatggt aaaaggacaa
     7681 tggtatgtag gcaaaattaa tgatgagaaa agagattata taatttatag aaatgctaca
     7741 tcagagtatt ggaattctat gc
//
